LOCUS       DASTQY010000001         3395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1611569, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000001 DASTQY010000000
VERSION     DASTQY010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611569"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFR82_00005"
     CDS             <1..269
                     /locus_tag="VFR82_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500006.1"
                     /translation="NTLTTPKPIRTIGSYWPYAPTLYDYIHRAMPFDAPQSLSPEEIY
                     SVVAWLLFQNHIIEEHEVIDAQTLPKVQMPNQNGFIPDPRPDVR"
     gene            399..2483
                     /gene="ftsH"
                     /locus_tag="VFR82_00010"
     CDS             399..2483
                     /gene="ftsH"
                     /locus_tag="VFR82_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU4500007.1"
                     /translation="MTITGQHMKRHSDAGPAGRVPPADTRPAAAKGNEGTTMPPRKMW
                     LWFLFLLFTNYLLVRLLMPGAEDAITVPYTVFKEEVTKGNVQAIYSRGDTITGRFGTP
                     ITYPPPRDQGTVSSGDGKGKAKRGWVPPDHPSKTASAFETTLPAFADQGLEAFLIENN
                     VEISAEPVQEGGNPWMTLLFSFGPALFLIGFYIWIFRRATQGAGMMGGGLMGIGKSKA
                     RRYDQEKDTKVTFDDVAGIDEAENELVEIVDFLRDTDKYTRLGGTAPKGVLLVGAPGT
                     GKTLLAKAVAGEAGVPFFSMSAAEFVEMIVGVGAARVRDLFKEAREHAPAIIFIDELD
                     SIGRARGRMAIGGASEQEQTLNQILTEMDGFSSREGIIVLAATNQPEVLDKALLRPGR
                     FDRRVVVNLPDKAGREAILKVHTRNMPLANDAILGDLAGITPGLSGADLKNLVNEAAL
                     LAARREQNDVRHKDFLDALEKIVLGPERPILMSRADKERIAYHEGGHAILGLVVPGAD
                     PVNRVTIVPRGQALGVTYQRPDSDRYNYPEAYLRARIVGMLGGRAAEEIVYGTKTTGA
                     ENDIEQATGLARRMVTRWGMSERIGLVQLAPKENAYLGSDGFPGEKPFSEETAKAIDA
                     EVLKIIGESHDEAKRLLIAHRKQLDALAEALLSRETLNEVEILEVTGLPPAPALQTAM
                     LDVPGGIKKSAV"
     gene            2700..>3395
                     /locus_tag="VFR82_00015"
     CDS             2700..>3395
                     /locus_tag="VFR82_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEU4500008.1"
                     /translation="MLDWLSNPEVWIALGTLTALEIVLGIDNIIFISVLVGRLPEAQR
                     AVARRLGLGLAMVARLGLLFSISWVMGLTQPWFALLGNEISGRDVILIGGGLFLLAKA
                     THEIHYSLEGVGEKDTAVLGSSLGMALVQISLLDIVFSLDSVITAVGLVEEVSVMAIA
                     IILAVVVMMIAAKSIADFVDEHPTIKILALSFLILVGVTLMVEGFDVHVPKGYIYFAM
                     AFSVAVEMLNIRMR"
BASE COUNT          726 a          888 c         1061 g          720 t
ORIGIN      
        1 aaaacacttt aacaacccct aagccgatca ggacgattgg cagctactgg ccctatgccc
       61 caacgctcta cgattatatt caccgggcga tgccatttga cgccccacag tctctcagtc
      121 ccgaggaaat ctattcggtc gtcgcgtggc tgctcttcca gaaccacatc attgaagaac
      181 acgaggtgat cgatgcgcag accttgccga aggtccagat gcctaaccag aacggcttca
      241 tcccggatcc tcgaccggac gtgcgataac gacgactagt catgcactgg agggtaggga
      301 taatagtagc tgcattggtg agttgatttt catccagtaa gcaggtttca tgcgtaagct
      361 tcgccggggc tattgagcat gagcgagagg agattttcat gaccataacg ggacaacaca
      421 tgaaacgcca cagcgatgca ggaccggccg ggcgcgtccc tcccgccgat acgaggcccg
      481 ccgccgcaaa gggcaacgaa gggacgacga tgccaccacg gaaaatgtgg ctatggttct
      541 tgttcctttt gtttaccaac tatttgttgg tgcggcttct catgccaggc gccgaagatg
      601 ccatcaccgt tccatatact gtctttaagg aggaggtcac gaaaggaaat gtccaggcga
      661 tttatagccg aggcgatacc atcacgggac ggtttgggac gccgatcacc tatccgccgc
      721 cccgcgatca aggcacagta tccagtgggg atggcaaagg taaggcaaaa cgggggtggg
      781 ttcctcctga tcatccttcc aaaacggcga gcgcgtttga gacaacactg cctgcctttg
      841 cagatcaggg cctggaagct tttttgattg agaacaatgt tgaaatcagt gccgaaccgg
      901 tccaggaagg gggcaacccg tggatgacgc tgttgtttag cttcggcccg gcactctttt
      961 taatcggctt ctacatctgg atttttcggc gagcgacgca gggcgccgga atgatgggag
     1021 gcggattgat gggcatcgga aagagcaagg cccgccgcta cgatcaagaa aaagacacaa
     1081 aggtcacgtt cgacgatgtc gccggcattg atgaagccga aaacgagctg gttgagatcg
     1141 tcgatttcct tagagatacc gataagtaca cccgcttggg tggaaccgct ccgaaaggcg
     1201 ttctcttggt tggggcgccc gggacaggga agacattgct ggcgaaggcg gtggcaggag
     1261 aggcaggggt gccgtttttc tcgatgagcg ctgcagaatt tgtggagatg attgtgggcg
     1321 ttggcgcggc gagagtgcgg gacctcttca aggaggcgcg ggagcacgct cctgcgatca
     1381 tcttcatcga cgagctggat tccatcggcc gcgcccgggg gcgaatggcg atcggcggcg
     1441 cgagcgaaca ggagcagact ttgaatcaaa ttctgaccga gatggacggt ttctcgagcc
     1501 gggaaggcat tatcgtattg gcggcaacga accagccgga ggtcctggac aaagccctcc
     1561 tgcggcccgg gcgcttcgac cgacgggtcg tcgtgaacct tccggacaag gccgggcgag
     1621 aggcgatcct caaagtccac acccgcaaca tgccgttagc gaatgacgcc atactggggg
     1681 accttgccgg catcacccca ggcctctcgg gggccgacct gaagaacctg gtcaatgagg
     1741 ccgcgctgct ggcggctcgc cgcgagcaga acgacgtccg tcacaaggat ttcctcgacg
     1801 cgctggagaa gatcgtgctt gggcctgagc gtccgattct tatgagccgt gcggacaagg
     1861 agcggatcgc ctaccacgag ggtggacatg ccattctcgg tcttgtggtg cccggtgccg
     1921 atccggtgaa ccgggtgacg atcgtgccgc gggggcaggc cctcggcgtg acctatcagc
     1981 gtcccgatag cgatcgctac aactatccgg aagcctatct gcgtgcgaga atcgtcggta
     2041 tgttgggtgg ccgggcggcc gaggagatcg tctacggcac gaagacgacc ggagccgaaa
     2101 acgacatcga gcaagccacc ggcttggctc gacgtatggt aactcgctgg ggcatgagtg
     2161 agcggattgg acttgttcag ctggcgccca aggagaacgc ttacctgggt tcggatggtt
     2221 ttccgggaga gaagccgttc agcgaggaga ctgcgaaagc catcgatgcc gaggtgctca
     2281 agatcatcgg cgagagccac gatgaggcca agcgactgct catcgcacat cgtaaacaac
     2341 tcgatgcgct cgcggaagcc ctattgtcgc gcgagacgct taacgaggtg gagatcctcg
     2401 aggtgacggg gcttcctcca gcaccggctt tgcagacggc gatgctggat gttcccggcg
     2461 ggatcaagaa gagcgcagtc taaggcgtgg cctgtggcct gcggtttatt gtcgccgcct
     2521 gcgtaaaccg caagtggcac tggcgaaccc ttcctcagct aaccccagcc aacaggaaac
     2581 aacacaacag ttgtagtcga ggcacaggcc ggcgctttgt ggtcgtgaaa gagtcagaac
     2641 cttttgcgct ctcttttcca gctccgacag gatggatgac ctaagcaaag gaggaggcga
     2701 tgcttgattg gctgtcaaat ccggaggtgt ggatcgcgct ggggactttg acagcgttag
     2761 aaattgtcct cggaatagac aacattatct tcatctccgt cctggtcggg aggctgccag
     2821 aggctcagcg ggctgttgcg agaagattgg gccttggctt ggcgatggtg gctcgtctgg
     2881 ggttgttgtt ttcaatctct tgggttatgg gtctgactca accgtggttt gcactgcttg
     2941 gcaatgagat ttccggtcgc gatgtgattc tcatcggagg tgggctattc ctcctcgcga
     3001 aggccacgca tgaaatccat tacagcctgg agggagtcgg ggagaaggat actgccgtac
     3061 ttggaagcag tcttgggatg gccttggtcc agatctcgct cctggacatc gtcttctcgc
     3121 tggattccgt gatcacggcg gtgggattgg ttgaggaggt atccgtcatg gccattgcca
     3181 ttatcctcgc ggtggtcgtc atgatgatcg cggcgaaatc tatcgcggat ttcgttgatg
     3241 agcatccgac gatcaaaatt ctcgccttgt cgtttctgat cctcgtcgga gtcacgctga
     3301 tggtggaagg gttcgatgtt catgtgccaa agggctatat ctattttgct atggcgtttt
     3361 cagtcgcagt cgagatgctg aacatccgca tgcgc
//
LOCUS       DASTQY010000002         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_402790, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000002 DASTQY010000000
VERSION     DASTQY010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402790"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFR82_00020"
     CDS             complement(<1..270)
                     /locus_tag="VFR82_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEU4500009.1"
                     /translation="MAIKVGINGFGRIGRNVLRASLGDPDLHFVAINDLTDAKTLAYL
                     LKYDSVHGTLKGSVEAKDDQIIVDGKAIKVLAIKDPKELPWKDLHV"
     gene            504..>2564
                     /locus_tag="VFR82_00025"
     CDS             504..>2564
                     /locus_tag="VFR82_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500010.1"
                     /translation="MTEADQTTQVLEWDKLLELLAAHTRSTRGAACVKSRALALDLAD
                     ARRWQQETVEMASFQDSVDPMPTLSFPDVEETLKRASKGAVLESHELRDQASVLALWE
                     EVQRYLGRHARHAPALASVAAALVSGPGLWKLKHALDASIDPDGSIRESATPELRRLT
                     HHANDLKQQIRDQLEHILRSSRYAEILQEQYFAQREGRYVVPIKIDMQRRVAGIVHDV
                     SSSGATVFLEPRELVDLNNAIKVADREVDREVLRILRDLSSRIAEQADVILMGMEALA
                     YLDAIAARAALGRQLQAHPVALNGDGRVRLKQARHPLLVLSKQQVVANDILLDDQVHI
                     LVISGPNTGGKTVTLKIVGLFALMVRAGMLLPCEPESEMAVFPELFADIGDAQDLARD
                     LSSFSAHMTHMVRLLKGKHGAGRALVLLDEPVTSTDPSEGAALAEALLCRLAAMGMKV
                     IVTTHYRSLKALAQTTPGFANASVEFDLETLSPTYRLFMGVPGGSSAIEIAGRLGMDS
                     AILDAARQKLHTDDRRVERLLEDLHAKHRKLTEDLAQAVKAKTEAEAAAEEAKARLAR
                     LEATEQEQRKGIKKKLQEQFSRARAEVQATLDEVKREHKLVKAKEAKERLVRIEVEAQ
                     AGLGPDAQQIPVEQLEAGDHVEIGGLGMTGKLLEPPQGKKRVRVKVGDGELLATVANL
                     VGIGR"
BASE COUNT          555 a          700 c          795 g          514 t
ORIGIN      
        1 gacatgcaaa tccttccagg gcagttcttt cgggtccttg atggccagca ccttgatcgc
       61 tttgccgtca acgatgattt ggtcatcctt ggcctcgacg cttcccttta gggtgccgtg
      121 cacggagtcg tacttcagca aataggcgag cgtcttggca tcggtcagat cattgatggc
      181 gacaaagtgc aaatcgggat cgccgagtga ggcacgcagc acattacgcc cgattcgtcc
      241 aaacccattg ataccgactt tgatagccat gataaagcag tccctcctaa actcggccgc
      301 gtgtgtctcg ttgaaacgac ggccgatagt atcggcggtt caccctctga gtcaagcgat
      361 acaaaagtgg taaccccgcg ttcgtagtga ggttttgact gactcgggct ccgtcgcatc
      421 taccagggta ggccaccttg cgactcgcta ccaggtccgt tacagttttt gactgattca
      481 cactgagcga acgatcgagg gctatgacgg aagccgacca gacaacgcag gtattggaat
      541 gggacaagct cctcgagttg cttgcggccc atacgcgctc cactcgcggt gcggcatgcg
      601 tgaagtcccg cgctttggcc ctcgaccttg cagacgccag gcgctggcag caggaaactg
      661 tggagatggc cagcttccag gacagtgtcg atccgatgcc gacgttgtca tttcctgatg
      721 tcgaagagac gctcaaaagg gcaagcaaag gtgcagtact agagagccac gaattgcgag
      781 atcaggcctc cgttttagcc ttgtgggaag aggtgcagcg ctatctcggt cggcatgcgc
      841 ggcatgcacc ggctttggcc agcgtcgccg ctgcgcttgt gtcgggtcct ggattatgga
      901 agcttaagca cgcactggac gcctcgatcg acccggatgg ctccatccga gaatcggcaa
      961 cgccggagtt gcgtcggctc actcatcacg cgaacgatct caaacagcag attcgcgacc
     1021 aacttgagca cattctacgt tcgagtcgat acgcggagat ccttcaggag cagtattttg
     1081 cacagcggga gggccgctat gtcgtcccga tcaagatcga catgcagcgc cgtgtcgcag
     1141 gtatcgtcca cgacgtctcg tccagcggcg ccaccgtgtt tttggagcct cgcgagttgg
     1201 tcgatctcaa caacgcgatt aaagtggccg atcgtgaggt cgatcgggaa gtgctgcgga
     1261 tccttcgcga cttgtccagc cgcattgccg aacaggccga cgtcattttg atgggcatgg
     1321 aagccctcgc ttacctggat gccatcgcgg cccgggcggc attgggtcga cagttgcagg
     1381 cccatccagt tgcgcttaac ggggacggcc gcgtacggct gaagcaggcg aggcatccgc
     1441 tgctcgtgct ttccaagcaa caagtcgtgg ccaatgacat ccttctagat gaccaggtcc
     1501 acatcctcgt gatatccgga cccaataccg gcgggaaaac cgtcacgctg aaaatcgtgg
     1561 gcctttttgc tctcatggtc agagccggga tgctcctgcc ttgcgagccg gaatctgaaa
     1621 tggcggtgtt cccagagctt tttgcggaca tcggagatgc gcaggatctc gctcgggact
     1681 tgtcgagctt ctctgcgcat atgacgcaca tggtccggct gctcaaaggc aagcatggcg
     1741 ctggccgtgc cttggtgctg ctcgatgagc cggtcacgtc gacagatccc agcgaagggg
     1801 ctgcgctggc agaggccttg ctgtgccgtc tcgctgccat ggggatgaag gtcatcgtga
     1861 cgacgcatta ccgttcgctg aaggcgttgg cacaaacaac gccgggattt gccaatgcga
     1921 gcgttgaatt cgatctggag acgttatcac ctacctatcg gctgttcatg ggagtgcccg
     1981 gcggctcgtc ggcaatcgaa atcgccgggc gtcttggaat ggactccgct atcctggacg
     2041 cggcgagaca aaaattacac acagatgatc ggagggtgga acggctgctc gaggacctgc
     2101 atgcaaaaca ccggaagttg acagaagatc ttgcgcaggc ggtcaaggcg aagaccgagg
     2161 ctgaggctgc ggcggaagag gccaaagcgc gactggcgag gttagaggcg actgaacagg
     2221 aacagcgcaa gggcattaaa aagaaattgc aggagcaatt cagccgcgcc cgagcggagg
     2281 tgcaagccac gctggacgaa gtcaaacgtg agcataagtt ggtcaaggca aaagaggcaa
     2341 aggagcggct ggttcggatc gaagtggagg cgcaagccgg tctgggccct gacgcccaac
     2401 agattccggt cgagcagttg gaggctggag accatgtaga aatcggcggc ctgggaatga
     2461 ccggaaagtt gctcgaaccg ccgcagggaa agaagcgcgt ccgcgtcaaa gtcggcgacg
     2521 gggagctttt ggcaactgtg gccaatttgg ttggcatcgg tcga
//
LOCUS       DASTQY010000003         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1610698, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000003 DASTQY010000000
VERSION     DASTQY010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610698"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFR82_00030"
     CDS             complement(<1..230)
                     /locus_tag="VFR82_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500011.1"
                     /translation="MRAWCGLGGLSLSLVMAVTTLAADPASMGPPFTVPVDSDGVQRT
                     TVILDSYSYQPGHLVVEKGKPVELTLKSVTTLT"
     gene            433..1347
                     /gene="htpX"
                     /locus_tag="VFR82_00035"
     CDS             433..1347
                     /gene="htpX"
                     /locus_tag="VFR82_00035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEU4500012.1"
                     /translation="MKWIKGIALFLVANFLIYLTLTFTANLLINVVLPAFGIDVRGAF
                     NEQLLVWSLVIGFGGAFISLLFSKQMARAMLSNAQQITSPRSHPEQMIYGSVQDIARR
                     LNITMPEVWVYESEDPNAFATGPSKNNSMVAVSTGLLQNLKEDEVRAVLAHEMGHVYN
                     GDMFTTTVLAGLMNTFVYYISNFLSSLVGQSGDREEGGAGNPFLGFIVYIVLQVVLSF
                     MAMLVVSWHSRRREFGADAFSAKVYGKGAMISALQAINRWVNRAQVEYGTQDPLATFK
                     ISGKTSGVMHLFATHPPMEERIAALERL"
     gene            1463..1957
                     /locus_tag="VFR82_00040"
     CDS             1463..1957
                     /locus_tag="VFR82_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU4500013.1"
                     /translation="MAAKTTSLYDFTMDDIDGKPVNLGDYKGKVLLVVNTASLCGNTP
                     QYTDLEHIYEQYHEKGFEVLAFPANNFGQQEPGTNAEIKGFCLTKYSVSFPLFSKISV
                     KGSDKHPLYQYLTEQSPFPGEVEWNFQKYLVDRSGNVVGRFHHRTKPGSSEVVKEIER
                     VLQK"
     gene            complement(1976..2260)
                     /locus_tag="VFR82_00045"
     CDS             complement(1976..2260)
                     /locus_tag="VFR82_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500014.1"
                     /translation="MGERIRVTDPDKLALFYERFRDVCLVEKEVWKEIFMPRDVMAGP
                     VRTNVQDRYEVEIDDQAIEDTLEANIPRGSSALAAAIQEYREHIAFYRKT"
     gene            complement(2285..2932)
                     /locus_tag="VFR82_00050"
     CDS             complement(2285..2932)
                     /locus_tag="VFR82_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500015.1"
                     /translation="MSIFGTPTRFQSRWLLACLLGFTSPMVGGWMCADARDLVAPKEQ
                     STTEIDPTQSPSFDEQKKGIPQSLFTWIKMAKGYDVEWDSFGRKGWYTQDPRLKPIEP
                     TSVFTPETPAIYIVFEVAPLEDPAQFSAQWFLETDGGKLPDAPIGKDSLEVPGHERYG
                     FLELKRKDDKWPLGKYQVKLFITPLGQQAFHAANQVGTMRFSIVEKLPAPSSAQK"
BASE COUNT          650 a          835 c          879 g          669 t
ORIGIN      
        1 gtgagcgtcg tgacgctctt gagcgtcagt tcgacgggct tgcctttctc gacgaccaaa
       61 tgccccggtt gataggaata gctgtcgagg atgacggtgg tgcgctgtac gccgtcggaa
      121 tctacgggaa cggtaaacgg tgggcccatc gaagcaggat ccgccgccaa ggtcgtgaca
      181 gccattacaa gagacagact gagaccgcct agcccacacc atgctcgcat aggaccctcc
      241 ttcgatgaga ggtttctaac aggaagcgtt ggaatcggtc aatgttcgct tggggttacg
      301 cggcgagccg gcatcttgac cttgtggccg cacgacttat tctagaatcc tgcgtattcg
      361 gttgtattat aatgtccgcg aggtaccacg tgccaagcgg tcggatgttc ccaccgcttt
      421 aaggaggaat cgatgaagtg gataaaggga attgccctgt ttctcgttgc caactttctc
      481 atctatctca cgctgacgtt tacggccaat ctattgatca acgtcgtctt gccggccttc
      541 ggcatcgacg ttcgcggtgc gttcaacgaa cagctgctgg tctggtcact ggtgatcggg
      601 ttcggaggcg cgttcatcag tttgttgttc tccaaacaga tggcgcgcgc catgctgtcg
      661 aacgcacagc aaattacctc gccgcggtcc catcccgaac agatgattta cgggtccgtt
      721 caggacattg cccgccgatt gaacatcacg atgcccgaag tatgggtgta tgagtcagag
      781 gatcccaacg ccttcgcgac gggtcccagc aagaacaact cgatggtcgc ggtgtccacc
      841 ggcctcctcc agaatctcaa ggaggatgag gtgcgggcgg tcctggccca tgagatgggc
      901 cacgtgtaca acggcgacat gttcacgacg acggtgctgg ccggattgat gaacacgttc
      961 gtgtattaca tcagcaactt cctgtcctcg ctggtcggcc aatcgggaga ccgagaggaa
     1021 ggcggagcgg gtaacccgtt tctcggtttc atcgtctata tcgtgctgca agtcgtgctg
     1081 tccttcatgg cgatgctggt cgtgagctgg cattcgcgac ggcgcgaatt cggcgccgat
     1141 gcgttttccg cgaaggtgta cggcaagggt gccatgatct cggccctgca ggccatcaac
     1201 cgctgggtga atcgcgccca ggtggaatac ggcacgcagg atccgctggc gacgttcaag
     1261 atttccggaa aaacctccgg agtcatgcac ctgttcgcca cgcatccgcc gatggaagag
     1321 cggatcgcgg ccttggaacg gctctaatac gaacggctgg gaggctgtca tgatgaaact
     1381 cgtcgttgcg ttgtcaacgg tcgcactgtg cggtggactc atgagcagcc ttccagccga
     1441 aggcaaagag gaaggacagc gtatggcagc caagaccacc agtctctacg attttacgat
     1501 ggacgatatc gacgggaagc cggtcaatct cggcgactac aaagggaaag tgctgctggt
     1561 ggtcaatacc gcaagcctct gcggaaacac gccgcaatac acggacctgg aacacatcta
     1621 cgagcagtac catgaaaaag gttttgaagt gctcgccttc ccggcgaata attttggcca
     1681 gcaggagccg gggaccaacg ccgagatcaa gggcttctgc ctcacgaagt acagcgtcag
     1741 ctttcccctg ttcagcaaga ttagcgtgaa gggttcagac aaacacccgc tctatcaata
     1801 tttgaccgaa cagagtccgt ttccgggcga ggtcgaatgg aacttccaga agtacctggt
     1861 ggaccgttca ggcaacgtgg tgggacgctt tcatcatcga acaaagcccg gctcgtctga
     1921 ggtggtgaaa gaaatcgagc gggtgcttca gaagtaggat ccggcacact gggtgctaag
     1981 ttttgcgata gaaagcaata tgctcgcgat actcctggat cgctgccgcc agcgctgacg
     2041 agcctcgagg gatattcgcc tccaacgtgt cctcaatcgc ctgatcatcg atttcgacct
     2101 cgtagcggtc ctggacgttc gtccgcaccg gacccgccat gacgtcgcgc ggcatgaaaa
     2161 tctccttcca cacctccttt tcgacgagac acacgtcgcg gaaccgttcg tagaacagcg
     2221 ccagcttatc cggatcagtc actctgattc gttcacccat gacgatcctt atgcaagggg
     2281 agaattattt ctgcgcggag gagggtgcgg gaagtttctc gacgatggaa aaccgcatgg
     2341 tcccgacctg attggctgca tggaaggcct gttgccccag cggcgtgatg aaaagcttga
     2401 cctgatactt tccgagcggc cacttgtcgt ctttccgctt gagttcgaga aaaccatacc
     2461 gttcatgacc gggaacttca aggctgtctt ttccgatcgg cgcgtcgggg agtttgccgc
     2521 cgtcggtttc gaggaaccac tgggcactga actgggcggg atcttcaagc ggggccacct
     2581 cgaaaacgat atagatggcg ggcgtctcag gggtgaacac ggacgtcggt tcgatcggct
     2641 tcagcctcgg atcctgagtg taccagcctt tgcgcccaaa gctgtcccac tcgacgtcat
     2701 agcccttcgc catttttatc caggtgaaga gcgactgggg aatgcctttt ttttgttcgt
     2761 cgaaggatgg actctgggta ggatcgattt ctgtcgtgga ctgctctttg ggagcgacca
     2821 agtctctggc gtcggcgcac atccatccac ccaccatcgg actagtgaat cccaagaggc
     2881 aggcgagcag ccaacgagat tggaaccgtg tgggtgtccc gaatatgctc atgcgagtct
     2941 cgccacccat ctccattcaa cacctatttc gtaccggacg caagccgtct gtgatagggt
     3001 gagtccggcc atcttttcat gagtcaccgt gtg
//
LOCUS       DASTQY010000004         7822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_800766, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000004 DASTQY010000000
VERSION     DASTQY010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800766"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..514
                     /locus_tag="VFR82_00055"
     CDS             107..514
                     /locus_tag="VFR82_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500016.1"
                     /translation="MTRIRPSLCVLALCTLAACSAATSSGRTVRTVEISDLVKPGVMY
                     ASPGDEVRWMNASGNPVRVGFLNRNLLEEHQCQNGIVNLFGEVNDLITIAPGESISLC
                     PVRAGDLRYNVWFDAENPRGSMSPTMTVRIRGG"
     gene            complement(777..2564)
                     /locus_tag="VFR82_00060"
     CDS             complement(777..2564)
                     /locus_tag="VFR82_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU4500017.1"
                     /translation="MYPYALIGNCHTSALINATGSIEWMCLPRPDSPPVFGRLLDPDG
                     GHFSITSSLSPSPEPTTSQSYLSNTNILTTTVKLSNGDSFRITDFCPRFEQYGRMYRP
                     AALFRLVEPLQGTPAIQVSCRPVCGWEKTAAMPVRGSNHLRYDIRGESLRLLTNMPLT
                     YLCEETPVALTRKFYFALTWGLGIEDDLIKVTHDFLEQTTRYWRLWVKHCSVPLLHQQ
                     EVIRSALALKLHCYEDTGAILAAMTTSLPEQVGGQRNWDYRYCWLRDAYFTLTAFNNL
                     GHFEEMESFLNFLLNIALTHEQSRDRLRPVYTLSQGLPLPEIEHSNWIGYKGSAPVRS
                     HNLAADQVQNDAYGEMILTFTPIFFDERFVDLRTRDLEGLLTHLAQLCARSIGRQDAG
                     LWEIRGGWQEHSFTNLLCWAGLERLERIKRAGFLKSITLDLTAERERASRALLRAVRD
                     GAVRNGPEDATHDAALSQLAILGYPDRALCDSTVGHIVRELSLACDRSETGFFYRYVR
                     EDDFGKPEGAFVVCSLWIAQALARLGNLNHARAILDRVVTAGNHVGLLSEHFIPRTKD
                     QCGNFPQAYSHVGLINAAFAVSPPWSDVL"
     gene            2613..4862
                     /locus_tag="VFR82_00065"
     CDS             2613..4862
                     /locus_tag="VFR82_00065"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEU4500018.1"
                     /translation="MRLSLRFLLPLALVLAGLAYAVIPLVDSLTLKWFVRDVEIRAEL
                     IGRMVEGPLGDLLASESRTKLPAYFNRIIQDERLFAIGFCDLRNRLLYKTQTYPEAIA
                     CEGNETIRAGETALLQLPRGAVHVAAVGIEGNAGPVGRLMLVHDMSWVQRRSTDTKWY
                     LFYLFAIIGAVISSVTVLVAHLSWRGWVTGVRSMLRGEGLIALIKDKHHGPELQPVAK
                     DLRALIHHLEADKRMKDESQVSWKPATLKSTLHDHLAGDEVLIVSNRQPYIHNRKDEA
                     IEVQVPASGVVTALEPIMRACSGVWIAHGNGTADRDVVDARAHVRVPPEAPSFDIRRV
                     WITPEEEAGYYYGFANEGIWPLCHLAHVRPVFRSSDWQQYQAINERFALAVVEEAKTD
                     NPVVLIQDFHLALVPKLVRDHLPHATIITFWHIPWPNPERYAICPWYVEILEGLLGSS
                     ILGFHTRFHCSNFINTVDRSLETRIDWDSSTISYGGKLTAVNPYPISIEWPGRMLASQ
                     PSVFECRKRIREIYALPLDHIVGLGIERLDYTKGILERFLAVERLLELEPEWIGRFSF
                     VQIAAPSRSKIDEYAQFRQQVENLAERINKRFGREGYQPIQLLIRHHDVRDVMMHYRG
                     ADLCVVSSLHDGMNLVAKEFVAARDDEQGVLILSQFTGAATELPDALVVNPYNIDQCA
                     AAMHLALTMPPTEQRARMRSMRGIVQEFNVYRWAGRMLMDAARMRQRARMVRQTSARN
                     ARTTTGGQA"
     gene            4859..5614
                     /gene="otsB"
                     /locus_tag="VFR82_00070"
     CDS             4859..5614
                     /gene="otsB"
                     /locus_tag="VFR82_00070"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEU4500019.1"
                     /translation="MIHALSEAGLCALRDAASRSLLYAFDFDGTLAPISSDRHAVTLP
                     PGVADWLRELAKRVPCAIVSGRALADVSLRTNGIVPHVIGNHGIESPLTSHSTLTEAE
                     RICADWKRELESTLAPMILLGVEIEDKRYSLTVHVRLAQDPADAARTSLAWLRRLSPE
                     PKLIEGKYSINALPPGLGGKGEAAFALMKHLGRTGLFYIGDEETDETVFALPDAVTMG
                     VRVGRQAESRAPFFLHEQSEVEELLRFLVQCMA"
     gene            5720..6970
                     /locus_tag="VFR82_00075"
     CDS             5720..6970
                     /locus_tag="VFR82_00075"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU4500020.1"
                     /translation="MDAADLVIVGGGAAGLAAAIAAAERTGGRHVALLDGAKTLGAKI
                     LISGGGRCNVTHEAVSATDFFGNRRIIKNVLAAFSVRETIDWFHSLGVELKREGTGKL
                     FPTTDKARTVLNALLNRCQALEVNMFTGHRVRRIECGAGGRNGFTVSHSQGTIHTKTL
                     ILATGGRSLPKSGSDGSGYALASDLGHSVTATVPALVPLLLDDKMFHRELSGVSQEVA
                     LTTVLAGKAIDVRTGSLLWTHFGVSGPVVMDASRVWTLARERGEQAEIYLNVLPGRTF
                     EQAKHWFIMQASESPRRSVVKTLALVVPERFAEAVCALLGCDPQRSAAQMPRSERDRL
                     LSVLTRLALPVTGDRGWNVAEVTAGGVPLEEINFRTMESKLVRGLYLIGEILDCDGRI
                     GGFNFQWAWASGHLAGCAAAASVS"
     gene            7018..7731
                     /locus_tag="VFR82_00080"
     CDS             7018..7731
                     /locus_tag="VFR82_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEU4500021.1"
                     /translation="MKTAARFDSLRMIRFFGHILTSLGSKVLIALSGLGLAGFVVFHM
                     LGNLQVFQGQDALNAYAAFLRDMPMALWSARLALLAIVGLHIGLSVRLAIQNRRARPA
                     GYVVHHYRRASLASRTMTLTGSLLVLFIVFHLVHLTAGWLDPEFHNRVDLRGQRDVFG
                     NIVHAFQNPLLVAIYLAGQAVLGLHLSHALSSSLQTLGLEHPMLDRLFRGAGPLVALL
                     VVVGNVAIILAIALGLVHA"
BASE COUNT         1549 a         2218 c         2448 g         1607 t
ORIGIN      
        1 ttgtttcatc ctcctctgat ctagtcgtcc tttccgtctc agcagcctct caaattacag
       61 cactcctgca ctgccgtccg agcgcgcagg agaggaaagg atgagaatga cacgaatacg
      121 tcccagcttg tgtgtgctgg ccctttgtac cctcgccgca tgcagtgcgg cgactagtag
      181 cggccgaacc gttcgaacgg tcgagatttc tgatctcgtc aagcctgggg tgatgtatgc
      241 ctcgccggga gacgaggtgc gatggatgaa tgccagcggg aatcccgttc gcgtcggatt
      301 tttgaaccgc aatctgctgg aagagcatca gtgccagaac ggcatcgtca acctgttcgg
      361 cgaagtgaac gacctgatta ccatcgctcc gggagagtcg atcagtctgt gtccagttcg
      421 tgccggcgac ctcagataca acgtctggtt cgatgccgaa aatcctcgcg gttcgatgag
      481 tccgacgatg acggtgagga tccggggagg atgacggtgg gtgcggctag tcagaacacg
      541 atgtgagaga tcatggctgg cggatggtcc aaatgttgtg attaccccga agcgtgagga
      601 catttgttcc aggatcggcc gcagcgcgcg aatagctgag ggagacagag gtctcggcat
      661 ggtgacgttc cgaggatgcg agaacgaggt ctgaggtgag cgcgcctcgc gcgtcgggag
      721 ggcgagtgcg tggcgctcct gtgggccggg tgaggagacg gcatgtcatc ctcgaattag
      781 aggacatcgc tccagggagg gctcacagca aacgcggcgt tgatcaaacc gacgtgggaa
      841 taggcttgag gaaaattgcc gcattgatcc ttcgtgcggg ggatgaagtg ctccgatagc
      901 agaccgacgt gattgcccgc cgtcaccacc cgatccagga tggcccgagc gtggttgaga
      961 ttgcccaggc gtgccagcgc ttgagcaatc cacaacgagc atacgacgaa cgccccttcc
     1021 ggcttgccga agtcgtcctc tcggacatag cggtagaaga accccgtctc cgagcgatcg
     1081 cacgcgagcg agagctcccg cacgatgtgc ccaaccgtgg aatcacacag tgcccggtcg
     1141 ggatatccaa ggatggccag ttgcgacagc gccgcatcgt gcgtcgcatc ctcgggtccg
     1201 ttgcggacgg cgccgtcccg tacggcgcga agcagggcgc gggaggcacg ttcgcgttca
     1261 gcggttaaat cgagggtgat cgatttgagg aaccccgcac gtttgattcg ttccaaccgt
     1321 tccaacccgg cccagcacaa gagattcgtg aaggagtgct cttgccaccc gccccggatc
     1381 tcccagaggc ctgcgtcctg acgcccgatg ctcctggcac agagttgcgc cagatgagtc
     1441 agaaggcctt cgagatcccg cgtgcggaga tcgacgaacc gttcatcgaa gaaaatcggc
     1501 gtgaaggtga gaatcatctc gccgtaggcg tcattttgga cttgatcggc cgccaggtta
     1561 tggctgcgga ccggcgcgct gcctttatag ccgatccaat tcgaatgctc gatttccgga
     1621 agcgggaggc cctgactgag cgtgtacact ggccggagac ggtcacggga ctgctcatgg
     1681 gtaagggcga tgttcaagag gaagttcagg aaggattcca tttcctcgaa atggcccaga
     1741 ttgttgaagg ccgtgagcgt gaaataggcg tcacgcaacc agcagtagcg ataatcccaa
     1801 ttccgctgac cgcccacctg ttcgggcagg ctggtcgtca ttgccgccag aatagccccg
     1861 gtatcttcgt agcagtggag cttcagtgcc agggcggagc ggatcacctc ctgctggtgg
     1921 agcaggggga cggaacaatg cttgacccag agccgccagt agcgggtcgt ttgttcaaga
     1981 aagtcgtgcg tcactttgat gaggtcgtcc tcgatgccca gtccccaggt cagcgcaaaa
     2041 taaaatttcc gcgtcaaggc gacgggggtt tcttcgcaga gataggtcaa gggcatattc
     2101 gtcagaaggc gcagtgactc accgcggatg tcgtaccgta gatgattgct gccacggacg
     2161 ggcatcgcgg cggttttttc ccatccgcac acgggacggc agctgacctg aatggccggt
     2221 gtgccttgca aaggctcgac aagccgaaaa agcgcggcgg ggcggtacat gcggccgtac
     2281 tgctcgaatc gaggacagaa gtccgtaatc cggaacgaat cgccgtttga aagcttgaca
     2341 gtcgtcgtca ggatgttggt attggaaaga taggattggc tggtggtcgg ctccggtgac
     2401 ggtgaaagcg agctcgtaat ggagaagtgg ccgccatctg ggtccaaaag cctcccgaag
     2461 accgggggac tgtcaggccg ggggaggcac atccattcga tacttccggt tgcattgatg
     2521 agggccgagg tatgacaatt accgatgagt gcgtagggat acatgctcca acaatagggg
     2581 attttgccgc tgaagtaaac gagaggtgat gtatgcgttt gtccctgcga ttcttgctgc
     2641 ctctggcctt ggtgctggcc ggcctcgcct atgccgtcat acccttagta gacagcctca
     2701 cgctgaaatg gttcgtgagg gatgtcgaaa tccgcgccga gctgatcggc cgcatggtcg
     2761 aaggtccgct cggtgacttg ctcgcatcag aatccaggac gaagcttccc gcctatttca
     2821 atcggatcat tcaggacgaa cgactttttg ccatcggatt ctgcgacctc cgcaaccgcc
     2881 tgctctacaa gacgcagacg tacccggaag cgatcgcctg cgaaggaaat gagacgatcc
     2941 gcgccggcga gacggccctc ttgcagttac cgcggggagc cgtgcacgtg gcggcagtcg
     3001 gcatcgaagg caacgccggt cccgtcggcc gtttgatgct ggttcatgac atgagctggg
     3061 tgcagcgccg aagcacggat acgaagtggt atctgttcta cctgtttgcc atcatcggag
     3121 cggtgatctc atcggtcact gtgctcgtgg cacatctgag ttggcgtggc tgggtcaccg
     3181 gggtccgttc gatgctgcgt ggggagggcc tgatcgcact gatcaaagac aaacaccatg
     3241 gtcccgagtt gcaaccggta gccaaagacc tccgtgcgct tatccaccat cttgaggcag
     3301 acaagcgcat gaaagacgaa agccaggtga gctggaagcc cgcaacgctg aaatccactc
     3361 ttcacgacca tttggccggc gacgaagtgc tcatcgtctc gaaccgccag ccgtacattc
     3421 acaatcggaa ggatgaggcg attgaagtcc aggttccggc cagcggagtc gtcacggcct
     3481 tggaaccgat catgcgggcc tgttcaggag tgtggatcgc ccatggaaac ggcaccgccg
     3541 atcgggacgt cgtcgatgca cgcgcacatg tccgcgtgcc gcccgaggcg ccctcgttcg
     3601 atatccggag ggtctggatc acgccggaag aagaagccgg gtattactat ggcttcgcga
     3661 acgaaggcat ctggcctctc tgtcatctgg cccacgttcg tcccgtgttt cgatcgtctg
     3721 attggcagca gtatcaagcg atcaatgaac gattcgcctt ggcagtcgtg gaggaggcca
     3781 agacagataa tccggtcgtg ctgatccagg atttccacct cgccctggtg cccaaactcg
     3841 tacgcgacca cctgccgcat gcgacgatta tcacgttttg gcacattccc tggccaaacc
     3901 ccgaacggta cgcgatctgt ccctggtatg tcgaaattct ggaaggcctg ctgggcagca
     3961 gcatcttggg attccacacc cgcttccatt gcagtaattt catcaacacg gtggaccggt
     4021 ctctggaaac gaggatcgac tgggacagct ccacaatttc ctatggcggc aaactgacgg
     4081 ccgttaaccc ttatccgatc tcgatcgaat ggcccggccg catgctcgct tcccaacctt
     4141 cggtcttcga gtgccgcaag cggatcaggg agatctacgc actgcccctc gatcatatag
     4201 tgggattggg catcgaacgt ctggattata cgaagggcat tctggaacgg tttttggccg
     4261 tggaacgtct gttggaactg gagcccgagt ggatcgggcg gttttccttt gttcagatcg
     4321 ccgcccccag ccgttcgaag attgatgaat atgcgcagtt ccggcagcag gtcgagaatc
     4381 tcgccgagcg tattaacaag cggttcggcc gcgagggcta ccaacctatc caattgttga
     4441 ttcggcacca tgacgtccgg gacgtcatga tgcattatcg tggggcggac ttgtgcgtcg
     4501 tcagcagcct tcatgacggg atgaatctgg tggcgaaaga attcgtggcg gctcgcgacg
     4561 atgagcaggg cgtccttatt ctaagccaat tcaccggtgc ggccacggaa ttgccggacg
     4621 cgctcgtcgt caatccgtac aatatcgacc agtgcgcggc ggccatgcat cttgcgctga
     4681 cgatgcctcc gacggaacag cgggcgcgga tgcgaagcat gagaggcatc gtgcaagagt
     4741 tcaacgtcta ccggtgggcg ggccgaatgc tcatggatgc cgcccgcatg cggcagcggg
     4801 cacgtatggt gcgacaaacc agcgcgcgaa atgccagaac cacgacggga gggcaggcgt
     4861 gatccatgcc ctctcggagg ccggtctttg tgcattgcgc gacgccgcat cccgatcgct
     4921 tctctatgcg ttcgacttcg acggcacgct tgctccgatc tcaagcgatc gccatgccgt
     4981 cactctgccg cctggcgtgg cagactggtt gcgtgagtta gccaagcggg tgccgtgcgc
     5041 catcgtatcg ggaagggcgc tcgccgacgt gtcgctgcgg acgaacggaa tcgttcctca
     5101 tgtaatcggc aaccatggca tcgaaagtcc gttgacgtct cacagcacgc tgacggaggc
     5161 cgagcgaatc tgtgccgatt ggaagcgcga actcgagagc acgctcgcgc cgatgatact
     5221 gctcggtgtg gagatcgagg acaagcgata ttcactgacg gttcatgtgc gccttgctca
     5281 agaccccgca gacgccgcac ggacatcact tgcctggctc cggcggctgt ctccggaacc
     5341 gaaactgatc gaaggcaagt attcgatcaa tgcgttgcct cccggcttgg gcggtaaggg
     5401 tgaggcagcg tttgccctca tgaaacatct cggccgaacc ggactgttct atatcggaga
     5461 tgaggaaaca gatgaaaccg tattcgcttt gccggacgcg gtcacgatgg gcgtccgtgt
     5521 cggcaggcag gccgagtccc gcgcgccctt cttcctgcac gaacaatccg aagtcgaaga
     5581 gctcctacgc ttcttagtcc aatgcatggc ctgatgcctc ccgcgtctgc aaatgcagga
     5641 gcgatcccgg agggaaacgg ccgaggaccc atcacggaac aggcgccgtg cctctcgagc
     5701 ctcgagtaag gtgcgcctga tggatgcggc ggatctcgtg atcgtcggag ggggagccgc
     5761 tggccttgcc gcggcgatcg ccgccgccga acgaacaggc ggtcggcacg ttgccctgct
     5821 cgacggggcg aagacgcttg gcgcgaagat tctcatctcg ggtggaggcc ggtgcaacgt
     5881 cacccacgag gcggtgtcgg ccaccgattt cttcggcaat cggcgcatca tcaagaatgt
     5941 gctcgctgcc ttttcagttc gagagaccat cgactggttt cactcattgg gtgtcgagct
     6001 caagcgcgaa ggcaccggca agctttttcc caccactgat aaggcgagaa ccgtgctcaa
     6061 cgcgctgctc aaccgttgcc aggccctgga agtcaacatg ttcacaggtc atcgggtgag
     6121 gcgcatcgag tgcggggccg gcgggagaaa cggattcaca gtcagccact cacagggaac
     6181 gattcacacc aagactctca tcctcgccac cggaggtcga tcgcttccca aatcgggaag
     6241 cgatggttcc ggctacgcct tggccagcga ccttggtcat tcggtcactg cgaccgttcc
     6301 tgccttggtt ccattactat tggacgataa gatgtttcat cgggagcttt ccggcgtgtc
     6361 gcaggaggtg gcgctcacaa cagtcctggc cggcaaagct atcgacgtcc ggaccggcag
     6421 tttgctctgg acccatttcg gtgtcagcgg gccggtggtc atggatgcca gccgcgtgtg
     6481 gacgttggcc cgtgaacggg gcgaacaagc cgagatctat ctcaatgtgc ttcccgggcg
     6541 gacattcgaa caggcgaaac actggttcat catgcaggcg tcggagagtc cgcgaaggtc
     6601 tgtggtgaag accttggcgc tcgtcgtgcc tgagcggttt gccgaggcgg tatgtgcgtt
     6661 gctgggttgc gatccacagc gatcggccgc gcaaatgccg cgaagcgaac gggaccggtt
     6721 gctcagcgtt ctgacgcggc tggcgcttcc ggtgaccgga gaccggggat ggaacgttgc
     6781 ggaggtgacg gcgggaggtg tcccgcttga agaaatcaat ttccggacga tggagtcgaa
     6841 attggtccgc ggtctgtatt tgattggcga aatactagat tgtgacggcc gcatcggcgg
     6901 cttcaatttt caatgggcct gggcgagcgg ccatttggct ggctgcgccg ctgcggcgtc
     6961 cgtctcgtga gcacgtttcc ggcagacact tgagctatgg aaccgggcgg agtacaagtg
     7021 aagacagcgg cgcgatttga ttcgttgcgc atgattcgat tcttcggtca cattctcacc
     7081 tccctcggca gcaaggtcct cattgccctt tcgggattgg gactggccgg attcgtcgtc
     7141 tttcacatgc tcggaaacct gcaagtattc caagggcagg acgccctgaa tgcgtatgcc
     7201 gcattcttgc gcgacatgcc catggccctg tggagtgcgc ggttggcgct gcttgccatc
     7261 gtcggtctgc atatcggcct gtcggtccgc ctggcgattc agaaccggcg cgcgcgtcct
     7321 gccggttacg tggtccatca ctatcgtcgg gcctccctgg cctcccgcac gatgacgctg
     7381 accggcagcc tcctggtcct gttcattgtc tttcatctgg tgcatttgac ggcaggctgg
     7441 ctggaccccg agttccataa tcgcgtggat ctccgcggcc agcgtgacgt cttcggcaat
     7501 atcgtgcacg ccttccaaaa tccgctgctg gtggcgattt acctcgccgg ccaagccgtg
     7561 ttgggactgc atttgagcca cgccctgtcg agcagcctgc agaccttggg cttggaacat
     7621 cccatgctgg atcgtctgtt ccgaggtgcc gggccgctgg tggcattgct ggtggtcgtg
     7681 ggaaatgtgg ccattatcct cgcgatcgcg ttggggctgg tgcacgcgtg atcaccctcg
     7741 attctaaagt gccgcatggc ccgctcgaga cgaaatggga ccgccaccgg ttcagtgaaa
     7801 agctcgtgag tccgacgaac aa
//
LOCUS       DASTQY010000005         6727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1608753, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000005 DASTQY010000000
VERSION     DASTQY010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608753"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..826)
                     /locus_tag="VFR82_00085"
     CDS             complement(38..826)
                     /locus_tag="VFR82_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4500022.1"
                     /translation="MAESHKHRARIFLHRGDLVQARAAWEAAVLDDRQAGNPQELSNS
                     LGNLGNACALSQDYDAAERCYREVLDIQRTQRNTHAIAHTLVHLGNLHIGTGHPEQAR
                     PYYLEALDLLHTLNDHRALGILYNNLALQEAREQQWKQAIESFSQALSYHRMVGNEEG
                     LAVTYSQMGRCFLDQGDLTSAERCLNNGSEHYIKLGAEVGEAAVLRVLAELYSRRGDL
                     VSAVRCLERTVLIATTYALPDLSRDSHELSRLKGLATQLQPDPD"
     gene            complement(828..1307)
                     /locus_tag="VFR82_00090"
     CDS             complement(828..1307)
                     /locus_tag="VFR82_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500023.1"
                     /translation="MALAQRYTRLVAVRQWDCLMQFDAALAAQAAFHNAQGELGADWE
                     SAVALEEAFSSQAGSAARDAYDALLNLAQRYPNAHSFQAFCIYITWQQVTEETLARHF
                     HTGLRLCEHFLASIDEKHRADVEQIRELRESFRAGLGMDEEDDLQVEYRRDTPKGGD"
     gene            1367..3088
                     /locus_tag="VFR82_00095"
     CDS             1367..3088
                     /locus_tag="VFR82_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEU4500024.1"
                     /translation="MEVKRSTEERTDPVDQIIQYHLQTKHHFNRYARALGYLDWANQP
                     DPFRRFEGTRLTTLPLLNPEHVPVSPAYSAIFVQEGVSPHPVTLDSLSRFLEFALALS
                     AWKRAGESEWALRSNPSSGNLHPTEGYVVLPSLEGLDARSGLYHYAPKEHALELRAEM
                     SADALASLLRSFPAHTFIIGLTSVHWREAWKYGERAFRYCHHDVGHAIGTTRIAASTL
                     GWQAVLLDGCNQNTVATLLGTDRTEDFADGEPEHADCLLAIWPRVQDSPHDPVPLFLD
                     PLIVKQVVKTTWRGKANRLSQEHGVHWDIIDTAAESTRKTQANEVTIELERAQTSFKG
                     SAPDGHVSAGQMIRQRRSAVAFDAATSISAATFFHMLDRVMPRVELAQSARPMPWDCW
                     PYDPAVHLMLFVHRIEGLPPGLYFLVRDPAKMAFLQGAMNAELTWQLAPGCPEDLPLY
                     WLLEGDARKLAVQVSCHQDIAGDSAFSLGMLAEFEGLLRERGAWWYPRLFWESGLLGQ
                     VLYLEAEAAGVRATGIGCFFDDPVHEIVAVKGLSLQSLYHFTIGGPVEDRRLMTFPPY
                     RHLNRQA"
     gene            3111..3971
                     /locus_tag="VFR82_00100"
     CDS             3111..3971
                     /locus_tag="VFR82_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500025.1"
                     /translation="MFKIKKKTDKPKAAVLYNIVEDYSEFDAPGNVTVCAMTLSEDLP
                     AHARVLSESYDVPFESMGQLLKEGGIYVYPQQSGLLTRGLFACLLGDADGIPKQTFVL
                     DVMQFAEAEQSARARSVSLAAAAKEVFYRTLPEELKLKLTQKNLGLDYRTLDRAVAKG
                     GDVKYIDFRKDWSPHFKRLCIMPDGRLVETGGLDEFARLHGISTAQAKLLVEQGGTLE
                     VGEEVLACQIVNGQPAVARFSAKNYSKAKELAAAKNLHIIDALSEVAYNDPAMMRSLV
                     RKELTGGRHA"
     gene            4021..4407
                     /locus_tag="VFR82_00105"
     CDS             4021..4407
                     /locus_tag="VFR82_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HEU4500026.1"
                     /translation="MGIFLASCAPKPILYPNQHYKDMGRDAADQAIEDCSQMAKDAGA
                     TPSRGKTGQVAGNTVGGGAVGSAAGAVGGAVVGHAGRGAMVGAASGATAGFLRGLFRR
                     TPPSQAYKEFVQRCLKERGYDPVGWE"
     gene            complement(4419..4781)
                     /locus_tag="VFR82_00110"
     CDS             complement(4419..4781)
                     /locus_tag="VFR82_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4500027.1"
                     /translation="MATILVIDDEDSIRHLLKDVLEKAKHRVLEARDGREGLNLYQSN
                     QIDLVLMDILMPGTDGLEATLQLTREYLDAKIIAMTGAQGDRNFLDVAKLFGARRVFE
                     KPFDLTKMVEAVNEELAR"
     gene            5150..5638
                     /locus_tag="VFR82_00115"
     CDS             5150..5638
                     /locus_tag="VFR82_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEU4500028.1"
                     /translation="MVSPTPIRAEADALPEGELSFYNVHTDERLRVRYRDERGHYDLS
                     ALDDLNHILRCHHTGEVAAMDPRVIEHLNLVQKTLGGEGEIHVISGYRSPEYNAILVK
                     RSRRAARHSLHVEGQAVDFYIPGVRPREIRQAALKLRYGGVGYYPRADFIHLDCGPFR
                     SW"
     gene            complement(5665..>6727)
                     /gene="glgB"
                     /locus_tag="VFR82_00120"
     CDS             complement(5665..>6727)
                     /gene="glgB"
                     /locus_tag="VFR82_00120"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HEU4500029.1"
                     /translation="RIFNYGRVEVRNFLLNSALSWLDRYHIDGLRVYAVASMLYLDYG
                     RNAGEWIPNQFGGHENLDAVLFLKDLNVLVHRDFPGAMMLAEESTSWAGVSRPTYTGG
                     LGFTFKWNMGWMHDMLEYFSHDPVHRIYHQNQLTFGLLYAFNENFMLPLSHDEVVHGK
                     GSLINKMPGDEWQQFANLRALYGLMYGHPGKKMLFMGGEFAQRQEWTPDHSLDWHLLQ
                     YGYHQGVQNLVRDLNRLYRSERALHAVDHDWQGFQWVDFHDAANSIVAFLRKAQDPDE
                     QILCVCNFTPVPRYDYRIGAPDEGFYRELLNTDASVYGGSNMGNGGGVHAHRHPSHGF
                     PCSLQLTLPPLSVTFLKAG"
BASE COUNT         1382 a         1835 c         2010 g         1500 t
ORIGIN      
        1 gctcgccgaa acaagcggct gtgcgagcaa atattgatca gtccgggtca ggctgaagct
       61 gagtcgcgag ccctttgagc cgactgagct cgtgtgaatc ccgcgacaga tccggaagcg
      121 catacgtggt cgcaatgagc accgttcgtt cgaggcaacg gacggcggat acaagatctc
      181 cgcggcggct gtagagctcc gccaatacac gcaggaccgc agcttctccg acttccgcgc
      241 ccagcttaat gtagtgctcc gaaccgttgt tcaagcatcg ttcggcactc gtcagatctc
      301 cctggtcgag aaagcatcgt cccatctggc tgtaggtgac ggccaagcct tcttcgttgc
      361 cgaccatacg gtggtagctg agtgcctgac taaacgactc gatcgcttgc ttccactgct
      421 gttcccgggc ctcctgaagg gcgagattgt tgtacaggat gccgagtgcc ctgtggtcgt
      481 tcaaggtgtg aagcaagtca agggcctcca gatagtacgg ccgcgcctgt tcgggatgtc
      541 ctgttccgat gtgcagattg ccgagatgga ccagcgtatg cgcaatcgca tgggtgttgc
      601 gttgcgttcg ctggatatcc agcacctcgc ggtaacatcg ttctgcggca tcgtagtctt
      661 gactgagcgc gcaggcgttg ccgagattgc cgagcgaatt cgacagttcc tgcggattgc
      721 ccgcctggcg atcatccagc accgctgcct cccaggccgc tcttgcctgt acgaggtccc
      781 ctcggtggag aaaaattctc gcgcggtgct tgtgactctc cgccatgcta atcgccgccc
      841 ttgggcgtgt ctcgtctgta ttccacttgc aaatcgtcct cttcatccat ccccaatccc
      901 gcccggaacg actcccgcaa ttcacggatt tgctcgacgt ctgcacgatg tttttcgtcg
      961 attgaggcga gaaagtgctc gcacagccgg agaccggtgt gaaagtggcg agcgagggtt
     1021 tcttccgtga cctgttgcca ggtgatatag atacaaaacg cttgaaagga gtgggcgttt
     1081 ggatagcgct gcgcaagatt caagagggcg tcataggcgt ctctggccgc gctgccggcc
     1141 tgggaggaaa aagcttcttc gagtgccacg gcgctctccc agtccgcgcc gagttcgccc
     1201 tgtgcgttgt gaaatgcggc ctgtgcggct agtgcagcgt cgaattgcat gaggcagtcc
     1261 cattgtcgga cggcaacgag tcgagtgtaa cgttgcgcga gcgccattcg caactcagcg
     1321 cgcgtttact tttctccacg tagctttgta cactggacgg cacggtatgg aagtcaagcg
     1381 gtcaacagaa gagaggaccg atcccgtcga tcagatcatc cagtatcatc ttcaaacgaa
     1441 acatcacttc aatcgatatg cgcgcgccct gggctatttg gactgggcga accagcctga
     1501 tcccttccgt cggtttgagg gaacgcgatt gacgacccta ccgctgctca atcctgagca
     1561 tgtccccgtc tcgccggcct attccgcgat cttcgtgcag gagggggtgt cgccccaccc
     1621 cgtgacgctg gacagtcttt cgagattcct tgagtttgca ttggccctct ccgcttggaa
     1681 gcgggcaggg gaatcggaat gggcattgcg cagtaacccc tcttccggaa atctccatcc
     1741 gaccgaagga tatgttgtac tgccctctct cgagggactg gatgcgcgat ccggcttata
     1801 tcactatgcg ccgaaggaac atgcgctgga attgcgagcg gagatgtcgg ccgacgcgtt
     1861 ggcgtcattg cttcgttcgt ttcctgctca tacctttata attggactga catccgtaca
     1921 ctggcgagag gcttggaagt acggcgaacg ggcgttccgg tactgtcatc atgatgtggg
     1981 acacgcgatt ggcacgacgc gaattgccgc ttcgaccttg gggtggcagg cagtgctatt
     2041 ggacgggtgc aatcaaaata ccgtggcgac gttgctggga acagaccgca ctgaagattt
     2101 cgcggatggg gaacctgagc atgccgattg tcttctggcc atctggccga gagtacagga
     2161 ttcccctcat gaccctgtcc cgttatttct tgatccactc atcgtcaagc aggtggtgaa
     2221 gaccacatgg agggggaaag cgaaccggtt gagccaggaa catggtgtgc actgggacat
     2281 catcgatacg gcagccgagt ccacgcggaa gacgcaggcg aacgaggtga cgatcgagct
     2341 tgagcgtgcc cagacttctt tcaaggggtc cgcgccggat ggccatgtgt ctgcagggca
     2401 gatgattcgg caacgacgca gtgccgttgc gttcgatgcg gcgacctcaa tttccgctgc
     2461 gacgtttttc cacatgctcg atcgggttat gccccgtgtc gaattggcgc aatcggctcg
     2521 gccaatgccg tgggattgct ggccgtacga tccggcggtt catctcatgc tcttcgtcca
     2581 tcgcatcgag ggcctgcctc ctggactcta tttcctcgtt cgtgatcccg cgaaaatggc
     2641 ctttctccaa ggggcgatga atgcagaact cacgtggcag ttggcgcctg gttgtccgga
     2701 ggatctcccg ctgtattggc tgttggaagg cgatgccagg aaactcgcgg tccaagtaag
     2761 ctgtcatcag gacattgccg gggacagtgc tttttctctg gggatgctgg ctgagttcga
     2821 ggggttgttg cgggaacgag gcgcgtggtg gtatccacga ctgttttggg aatctggcct
     2881 actgggacaa gtgctgtacc tcgaagcgga agcggcgggg gtgcgggcca caggcatcgg
     2941 atgttttttt gacgacccgg tccatgagat tgtggctgtg aaggggttga gccttcaatc
     3001 cctctatcac ttcaccatcg gaggtcctgt cgaggatcgc cgactgatga cctttccgcc
     3061 gtatcggcat ctgaatcggc aggcgtgaag cgagcgaacc ggagcgagcg atgttcaaga
     3121 tcaagaagaa gactgacaag ccgaaagccg ctgtgctcta caacatcgtg gaggactatt
     3181 cggagtttga tgcgccgggc aatgtgaccg tctgtgccat gaccctgagt gaagacctgc
     3241 cggctcatgc gagagtgctt tcagagagct acgatgtgcc ttttgagagc atgggtcagt
     3301 tgttgaaaga gggtgggatc tacgtctatc cccaacaaag cggccttctc acccgcgggt
     3361 tgttcgcctg cctactgggc gatgctgacg gcataccaaa gcagacgttc gtgctggatg
     3421 tgatgcagtt cgccgaagcc gaacagtcgg cgcgcgcacg atcagtttcc ctggcggcgg
     3481 ccgccaagga ggtgttttat cggacgttgc ctgaggaatt gaagctgaag ctgacccaaa
     3541 aaaacttagg cctcgattac cgcacgttgg accgagcggt tgccaagggg ggcgatgtga
     3601 agtacatcga ctttcgaaaa gattggtcgc ctcacttcaa gcggttgtgc atcatgccgg
     3661 acggccgtct cgtcgaaacg ggcgggttgg atgaattcgc ccgactgcac ggcatttcca
     3721 ctgcgcaggc gaaactgctt gtggaacagg gagggacgct ggaggtgggt gaagaagtgc
     3781 tggcttgtca gatcgtcaat ggccagcccg ctgttgcccg gtttagtgcg aaaaattatt
     3841 cgaaggcgaa ggaactggcc gccgcaaaaa acctccatat aatagatgcg ctgagcgaag
     3901 tggcgtataa cgatccggcc atgatgcgga gccttgtgcg caaggaactg acgggcggcc
     3961 ggcacgcatg atcggaagcc ttgcttgatg atgtggagga tcgtcagtag gtggctcctt
     4021 gtgggaatct tcctcgcttc ctgcgcccca aaacctatct tgtatccgaa ccagcactac
     4081 aaggacatgg gccgagatgc tgcagaccag gcgatcgaag attgcagtca gatggctaag
     4141 gacgcaggag cgactccaag ccgaggaaaa accgggcaag tcgccgggaa tacggtaggg
     4201 ggtggagcag tagggtcagc ggctggtgcg gtaggaggag cggtcgtcgg acatgccggc
     4261 cgcggagcca tggtcggagc cgccagtggc gcgacggcgg gttttcttcg cggtctgttc
     4321 aggcggacgc cccctagcca ggcgtataag gagttcgtgc agcgatgtct gaaagagcgt
     4381 gggtacgatc cggtcgggtg ggagtaagag aaacgggatt atctggctaa ttcttcgttg
     4441 accgcttcaa ccatcttggt cagatcgaac ggcttttcga agacacggcg agccccgaac
     4501 agtttggcca cgtcgaggaa gttgcggtcg ccctgcgcac ctgtcatcgc gatgattttc
     4561 gcgtccaagt attcgcgtgt cagttgcagg gtcgcttcca acccgtcggt gcccggcatc
     4621 aaaatatcca tgagcacgag atcaatctgg ttgctctgat acaggttcaa cccttcgcgt
     4681 ccgtcgcgcg cttcaaggac ccgatgtttg gccttctcca gcacgtcctt taataagtgc
     4741 cggatcgaat cctcatcatc gataaccaaa atggttgcca tgcccatggc tccttataag
     4801 tgacgtgata ttactcgtct tcctctgacc tgtctagcgg gaaatgtctc tcgcgcctca
     4861 ctcacggagt cctcgtgtga ctgtcacgag tttttggcaa agagtccctt agggaagtat
     4921 acaggtcttg tgattcgtcc atcagcgaag cggtccatcc gggtacgctc gcgaagaaaa
     4981 aactcggttg acctgggccg gcctatttga tacaagcctc cgaatacgta cgtatcctcg
     5041 ctgagtcatc ttttttctgg agcgatccgt gccagcctcc gattggtcgt ggacgagacg
     5101 aacagtcttg aaaacttcag tggccagtgt ccttctctgg agcgcacgtg tggtttcgcc
     5161 cacaccgatc cgtgcagagg ccgacgcctt acccgagggc gaactgtcat tttataacgt
     5221 gcacaccgat gagcgcttgc gggttcggta tcgcgatgaa agaggacact acgatctttc
     5281 ggcactggac gacctgaacc acatcctccg ctgtcatcat accggcgaag tggccgccat
     5341 ggatcctcgt gtcatcgaac accttaatct cgtccagaaa acgcttggcg gggaaggcga
     5401 gattcacgtc atttccggct atcggtctcc ggaatacaat gccatcctgg tgaaacgaag
     5461 ccgtcgggcc gcccggcata gtttgcatgt cgagggccag gccgtcgact tttacatccc
     5521 cggagtacgg ccccgtgaga tcagacaggc tgccttgaaa ctacgatatg gcggggtggg
     5581 gtactatcct cgtgcagact tcatccatct ggattgcggg cctttccgtt cctggtaatt
     5641 cccttcacgc tcccaccaat cctttcagcc ggccttgaga aacgtcaccg acagcggtgg
     5701 caatgtgagt tgcagtgaac aaggaaagcc gtggctgggg tgcctgtgcg catggacccc
     5761 gcctccattg cccatgttgc ttccaccgta cacggaggca tcggtgttga ggagctcacg
     5821 gtagaaccct tcatcaggcg cgccgattcg atagtcgtac cgcggaacag gagtgaagtt
     5881 gcacacgcag aggatctgct catctggatc ttgggctttc ctcaagaatg cgacgatgga
     5941 gttcgccgcg tcatgaaaat ccacccactg gaatccttgc caatcatggt cgacggcatg
     6001 gagggcgcgc tcggaccggt agagccggtt gaggtcccgt accaggtttt gcactccttg
     6061 atggtatccg tattgaagca ggtgccagtc gaggctgtgg tcgggggtcc attcctgccg
     6121 ttgcgcgaat tcgccgccca taaagagcat tttttttccc ggatggccat acatgagacc
     6181 gtacagtgcg cgcaagttgg caaactgctg ccattcgtca ccgggcattt tattgatcag
     6241 cgaccctttg ccgtgcacga cttcgtcgtg cgaaagcggg agcatgaagt tttcgttgaa
     6301 ggcatacagc aatccgaacg tcagctggtt ttgatggtag atccgatgca caggatcgtg
     6361 actgaaatat tccaacatgt cgtgcatcca gcccatgttc cacttgaagg tgaatccgag
     6421 gccgcccgta taggtcggcc gggagacccc ggcccaagac gtggattcct cagccagcat
     6481 catggcaccc gggaagtcgc gatggacaag gacattcaag tctttcaaaa agagcacagc
     6541 gtcgagattt tcatgtccgc cgaactgatt ggggatccac tcacccgcgt tgcgtccgta
     6601 atccagatag agcatggagg cgacggcgta cacacgaagc ccgtcgatgt gatagcggtc
     6661 taaccaggag agtgcgctgt tcaacagaaa attccgcacc tcaacgcgtc cataattgaa
     6721 aatgcgg
//
LOCUS       DASTQY010000006         8626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1408459, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000006 DASTQY010000000
VERSION     DASTQY010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408459"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VFR82_00125"
     CDS             complement(<1..367)
                     /locus_tag="VFR82_00125"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEU4500030.1"
                     /translation="MPICPHGSYDGRISLLTITGGLLSGFRFSPAFVSALLVTAIVIG
                     CSSPPKPIYFPGYPIGYVEGGVASWYGPGFHGNRTANGERYDMHKLTAAHRTLPLGSI
                     ATIRSTTTGREVTVRINDRG"
     gene            complement(405..815)
                     /locus_tag="VFR82_00130"
     CDS             complement(405..815)
                     /locus_tag="VFR82_00130"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU4500031.1"
                     /translation="MPTTTQLVISGQSRPGVMAEVASVLAAAGVNIKAFSAPEVTGTG
                     KLRLLVADVEGARTALRGAKIKFSEETALILSLENKPGALNEVADLLTRARINIKCGY
                     CTPSREGKRAIVVLTVANTDKALSILRDQSLDEF"
     gene            complement(910..1317)
                     /locus_tag="VFR82_00135"
     CDS             complement(910..1317)
                     /locus_tag="VFR82_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500032.1"
                     /translation="MTTSLHEQGIETVHDQVVSQVAQRWAKAFVCKATIKGTGEHHSW
                     ASPDQTCDIVGWYVSSGGDTMEWMAEVETEESLSDPQIVEKWMRTITRGIPFYLLVPR
                     GMKESAQKLTQDASIVISYIYEFTFINGVCHIL"
     gene            complement(1373..4912)
                     /locus_tag="VFR82_00140"
     CDS             complement(1373..4912)
                     /locus_tag="VFR82_00140"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEU4500033.1"
                     /translation="MRIERRFTRSGQSPYEGLKFVKRASEIRNPDGSTVFKLDNIDIP
                     ESWSQLAIDILAQKYFRKAGVPQRDDAGNPIMGHDGKPALGGERDARQVFERMAGCWT
                     HWGKTYGYFKTIEDSAAFHDELCFMLAHQMAAPNSPQWFNTGLHYAYGLSGPAQGHFY
                     VDPKTREVVKATNAFEHPQPHACFIQSIEDDLVNENGIMDLWVREARLFKYGSGTGTN
                     FSKLRGDGEALSGGGRSSGLMSFLRIGDRAAGAIKSGGTTRRAAKMVCLDLDHPDIEE
                     FIDWKVVEEQKVAAMVTGSKICAQRLNAVLKACHIVDAEGIPHVSVDPKQNSILREAL
                     TAARRDNVPEAYIQRMFSYAQQGFTHFVFHEYDTNWDGKAYQTVSGQNSNNSVRIPNE
                     FFAALEADSDWQLKRRIDGKVAKTVKARDLWDRVAWAAWICADPGTQYDTTINEWHTC
                     PEDDRINASNPCSEYMFLDDTACNLASLNLAQFFTSEGEFELEHFRHAVRIWTIVLEI
                     SVLMASFPSRSIAEKSYQFRTLGLGYANLGTILMRQGIPYDSDKALAICGALTSIMTG
                     EAYSTSAEMAAELWPFPGYAKNREAMLRVIRNHRRAAYSAPEEEYEGLTIKPTSIHAE
                     HCPPDLLIAARRAWDRALELGTAYGYRNAQVTVIAPTGTIGLVMDCDTTGIEPDFALV
                     KFKKLAGGGYFKIINQSLPPALSTLGYTEAQIQDIVNYCVGRQTLQGAPFINHETLKQ
                     KGFDDAALARMESGLSQAFEIQFAFNKYVLGEEFCRAKLGLTDVQLMEANFNMLKALG
                     FTQEEIAAANDYCCGAMTVEGAPHLKSDHLPVFDCANRCGRIGQRYIAVDAHIRMMAA
                     AQPFISGAISKTINMPADATLEEVKSAYLFAWKSMVKAVALYRDGSKLSQPLSASSDG
                     GKSLETSTEVLTIAEKITERVLVRYLAKRRPLPARRNGYTQKAVIGGHKLYLRTGEYE
                     DGTVGEIFLDMHKEGAAFRSLMNCFAIAISLGLQHGVPLEEFVEAFVFTRFEPNGPVK
                     LNDRIKMSTSIIDYIFRELAVTYLDRYDLAQVKEEDLRMDSVKKDDQDPECSDEEADP
                     QMLASSSINTEMFPSRRGIQPRHGGNGHGNGNGSVTHKVELKRETLTMTAVQIARQKG
                     YEGDPCSECKQFTMVRNGTCLKCETCGATSGCS"
     gene            complement(5253..5624)
                     /locus_tag="VFR82_00145"
     CDS             complement(5253..5624)
                     /locus_tag="VFR82_00145"
                     /inference="COORDINATES: protein motif:HMM:NF025629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4359 domain-containing protein"
                     /protein_id="HEU4500034.1"
                     /translation="MTLFRLGMLVTALSVCIGLALSNPTIDEYLTFVESELAKAIERS
                     DGTQLSPERAMVRTIFRSHSHELVDSVVRPHTVRRNWGLVSVYDSTVFNSRIVVLGVA
                     GHFIPLQGIDEAIVRLGRLAF"
     gene            5726..6025
                     /locus_tag="VFR82_00150"
     CDS             5726..6025
                     /locus_tag="VFR82_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500035.1"
                     /translation="MFVWSKKLIASLLALFLVLLGVFLIHGAPDSASGLKIHTTRWMA
                     LNYIGLMIWVFVWMIDQTRMRGKNVWIWLMPFALAPLPTLMLFVLFLQRRLKPVS"
     gene            complement(6009..7283)
                     /locus_tag="VFR82_00155"
     CDS             complement(6009..7283)
                     /locus_tag="VFR82_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4500036.1"
                     /translation="MAADSFAHILANKRVALMLPLGFASGLPLALTSGTLQAWLTVAG
                     VDLKTIGIFTLVGLPYSLKFLWAPLIDRISLPWLGRRRGWMLITQIAVASGLALMGFI
                     GSNESPHWLGLMAVGVAFFSASLDIVFDAYRTDLLLPAERGFGAALWVNGYRLAMLVA
                     SAGALVLAEQIGWKGTYLVLAALMGAGVATILLSPNPPGEGAAPRTLKEAVGFPLREL
                     FARPGIYGLLCLIVLYKLGDAVAGSLQTAFFIGGLGFSTGEVGYVKGVGIAATLIGAL
                     AGGLIMAKTGLIRSLLFFGLLQALSNLSFMVLAWIGKSYEALAASVVIENVTGGMGTA
                     AFVALVMSLCDHRYTATQFALLSSLEALGRVFAGRPSAEAVGMLGWGWFFFLSFVLAL
                     PGCWLVWLYRRQLPPDISSDETSPESPVMKPA"
     gene            complement(7402..7818)
                     /locus_tag="VFR82_00160"
     CDS             complement(7402..7818)
                     /locus_tag="VFR82_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500037.1"
                     /translation="MRTPVTLRSFTACCVFSLSILVQEQVWSKDVLPIEPDLSTRIDE
                     LYDHEARLFLMLYSLKGDGQVDYVTGRMVQEYARSNFGNPVYQTEIHPLFYWWNHNMW
                     NDPEQDGVNGNERIYQENIEFDVSRYKPCTFNGQPC"
     gene            7932..>8626
                     /locus_tag="VFR82_00165"
     CDS             7932..>8626
                     /locus_tag="VFR82_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU4500038.1"
                     /translation="MIKRASLHSLGCRLNQAETSVLASQLRRQGYALVDFGEPTDLLV
                     VNTCSVTEEAERTCRYAIRKTLKHSPDAFIAVTGCYAQTGLQSLRSIPGIDLIVGNQF
                     KSDLPSFLPPPQTLRKQPAPEVLHTKTIDRDDFVLPEFGEPHSTRALVKIQDGCNVMC
                     SFCLIPFARGRERSRKVEDILSEAATLAAAGHHELVLTGVNIGQFSQSGATLADLIDR
                     LERIHGIQRIRLSS"
BASE COUNT         1946 a         2410 c         2354 g         1916 t
ORIGIN      
        1 ggccgcgatc gttgattcgg acagtgactt cccggccagt ggtggtcgag cggatcgtag
       61 caatggatcc gagcggcagt gtcctgtgag ccgccgtgag cttatgcata tcgtaccgct
      121 caccattggc ggtgcgattc ccgtggaatc caggtccgta ccaggaagcc actcctcctt
      181 cgacataacc gatggggtac ccgggaaaat agatgggttt aggcggactg ctacacccta
      241 tgactatggc agtcacaagc aaagcgctga caaaggccgg tgagaaacgg aagccggaga
      301 gcaaaccacc agtgatggtc agtaaggaga tgcgcccgtc ataggatcca tgcgggcaga
      361 tcggcatcag ccaagtacca gagacaatga agcgaacatg gctgctagaa ctcgtcgagg
      421 gactgatccc gcaggatcga gagcgctttg tcggtattgg cgaccgtgag aacaacgatc
      481 gcacgcttgc cttccctgga gggggtacaa tacccgcatt tgatgttaat gcgagcccgc
      541 gtcaacaggt cggccacctc gttcagcgct cccggcttgt tttcgaggct cagaatgagg
      601 gcggtttctt cgctgaactt aattttggct cctctcaagg cggtgcgcgc cccctcaaca
      661 tcggcgacta atagcctgag tttaccggtt cctgtgactt ccggagccga aaacgcctta
      721 atgttcacac ctgctgcggc caacacggag gcgacttcgg ccatgactcc cgggcgactc
      781 tgtccgctaa tgacgagttg tgtggtggta ggcatataac tatcctaatt ataacccccg
      841 cttaagagtc ctatcacttc gggcggggga atcgcaacag taaccgtcaa tctgggcagc
      901 cctcctaggc tacaaaatgt ggcagacccc gttaataaag gtgaattcat agatgtagct
      961 tatgacgata gaagcatcct gcgtcagttt ttgggcggat tccttcatgc ctcgtggcac
     1021 caataggtaa aaagggattc ccctcgttat cgtccgcatc cacttttcaa cgatctgtgg
     1081 gtccgacaat gactcttcag tttcaacttc cgccatccat tccatcgtgt cgcccccgga
     1141 gctcacgtac cacccaacaa tatcgcaagt ctggtcggga ctcgcccagg aatggtgctc
     1201 gccagtccct tttatggtgg ctttgcatac gaaggccttg gcccatcgtt gagccacctg
     1261 gctgacgact tgatcatgaa ccgtttcaat gccctgttcg tgaagcgaag tagtcatgtc
     1321 ctacggtcct aataattcga gacctctcct tcttcgaggg agcggcgagt ggttatgagc
     1381 atccgctggt tgcaccgcaa gtttcgcact ttaaacacgt gccgttgcgc accatggtga
     1441 attgcttaca ttccgaacag ggatcgccct catagccttt ttgccgcgca atctgtacgg
     1501 ccgtcatcgt cagcgtttcg cgcttgagct ccaccttgtg tgtgacgctg ccattgccgt
     1561 ttccatggcc gttgccgcca tgtctcggct ggatccctcg acgggacgga aacatctctg
     1621 tgttaatgga tgaggacgca agcatctgcg ggtcggcctc ttcatcagaa cattcgggat
     1681 cctggtcgtc ctttttcacg gaatccatcc gcaagtcttc ttccttgacc tgtgcaagat
     1741 cgtatcgatc caaatatgtc acggccagct cacggaaaat ataatcaatg atcgacgtcg
     1801 acatcttgat ccgatcgttc agcttcacag gtccgttggg ctcaaagcgc gtgaacacga
     1861 aggcctcgac gaactcttcc aggggaacgc cgtgctgcag ccccaacgaa atggcgatcg
     1921 cgaagcagtt catcaagctg cggaacgcgg ccccttcctt gtgcatgtcg agaaaaattt
     1981 cgcccactgt gccgtcttca tattcaccgg tacgcagata cagtttgtgc ccgccgatga
     2041 cagccttctg cgtatagcca ttgcgtcgtg ccggcagcgg ccgtcgcttg gcaaggtagc
     2101 gcaccaggac ccgttcggtg attttctcag cgatggtgag cacctctgtc gaagtttcga
     2161 ggcttttccc accatcactg gatgccgaca acggctgact cagcttggaa ccgtcgcggt
     2221 acaacgccac ggccttgacc atgctcttcc aggcgaagag atacgcggac ttcacctctt
     2281 ccagcgtagc atccgcaggc atgttgatcg ttttgctgat ggcgccgcta atgaacggct
     2341 gcgcggcagc catcatgcgg atgtgggcat caacagcgat gtagcgctgg ccgattctgc
     2401 cacaacgatt ggcgcagtcg aatacgggaa gatgatcgga tttcagatgt ggcgccccct
     2461 cgaccgtcat cgccccgcag caatagtcat tggcggccgc gatctcctcc tgggtgaatc
     2521 cgagcgcctt cagcatgttg aaattggctt ccattagttg gacgtcagtc agtccgagct
     2581 ttgctcggca gaattcctca ccgagcacgt acttgttgaa ggcgaactgg atctcgaagg
     2641 cctgtgacag gccgctttcc atacgggcca gcgccgcgtc atcgaaccct ttttgtttca
     2701 gcgtctcatg attgatgaat ggcgcacctt gcaaggtttg ccggccgaca caatagttga
     2761 caatgtcttg gatctgcgcc tcggtataac ccaacgtgct gagagcgggc ggcaagctct
     2821 gattgatgat cttgaaatag cctcccccag ccagcttctt gaatttgacc aaggcaaagt
     2881 ccggctcaat gccggtcgta tcacagtcca tgacaagacc gatcgttccc gtcggggcaa
     2941 tgaccgtcac ttgcgcgttg cggtaaccgt aggcagtacc gagctcgaga gcacgatccc
     3001 aggcccgcct ggcggcaatg agcagatcgg gcggacaatg ttccgcgtgt atgctcgttg
     3061 gtttgatcgt caatccttca tattcctcct ccggggcact ataggcggca cgacgatggt
     3121 tgcgaatcac gcgcagcatc gcctcgcgat tttttgcgta gccagggaac ggccagagtt
     3181 cggccgccat ttctgctgag gtactatagg cttcaccggt cataatggaa gtgagcgcgc
     3241 cgcagatggc caaggcttta tccgaatcgt atggaatgcc ctgccgcatg agaatcgtgc
     3301 cgagattggc atagccaaga ccgagcgtcc ggaactgata gctcttctcc gcaatggaac
     3361 ggctggggaa ggacgccatc aggacactga tttccaacac gatagtccag atccggaccg
     3421 catggcgaaa gtgctccagt tcgaattcgc cttccgacgt gaagaactgc gccagattca
     3481 acgacgccag gttgcaggcg gtgtcgtcga ggaacatata ttcggaacag gggttggagg
     3541 cgttgatgcg gtcgtcctcg ggacaggtgt gccattcgtt gatggtcgtg tcgtattgcg
     3601 tgcccggatc ggcgcagatc caggcagccc acgccacgcg atcccacagg tcccgagctt
     3661 tcacggtttt cgcgactttc ccgtcgatcc gccgcttgag ctgccaatcg ctgtccgctt
     3721 ccaaggcggc aaagaattca ttgggaatcc gtacgctgtt attggaattt tgtccggaaa
     3781 cggtctggta ggccttcccg tcccagtttg tatcgtattc atggaacacg aaatgggtga
     3841 atccctgttg agcataagag aacattcgtt ggatataggc ttccggaacg ttgtcacggc
     3901 gagccgccgt cagcgcttcc cttaggatgg agttctgctt cgggtcaacg gagacatgcg
     3961 ggattccttc cgcatcaacg atatgacagg ctttcaacac ggcgttcagg cgttgggcgc
     4021 agatcttcga tccggtcacc attgcggcaa ccttttgctc ttcgacgact ttccagtcga
     4081 tgaattcttc tatgtcggga tggtccaagt ccaggcagac catttttgcc gcgcgacgcg
     4141 tggtgcctcc tgatttaatc gcgcctgcgg cacggtcgcc gatgcggaga aatgacatca
     4201 gaccggagga gcgcccaccg ccggacaggg cttcaccgtc tccgcgcagt ttcgaaaaat
     4261 ttgttcccgt ccctgatccg tacttgaaga gccgcgcctc acgaacccac aggtccatga
     4321 tgccgttctc gttgaccagg tcatcctcga tcgactgaat aaaacaggcg tgcggttgcg
     4381 ggtgctcgaa ggcatttgtc gccttgacga cctctcgagt cttcgggtcg acgtagaaat
     4441 gaccctgcgc cgggccggat aatccatatg cgtaatgtaa ccctgtattg aaccactgcg
     4501 gcgaattggg agcggccatt tgatgtgcca gcatgaagca gagctcgtca tggaacgcgg
     4561 cactatcctc gattgtcttg aagtagccat aggtttttcc ccaatgtgtc caacaaccgg
     4621 ccatgcgttc aaagacttgt ctggcatcgc gctctccgcc cagcgctggt ttaccgtcgt
     4681 gtcccatgat gggattaccg gcatcgtcgc gttgcggcac ccctgctttg cgaaagtatt
     4741 tttgcgccag gatatctatc gcaagctgcg accaggattc cggaatgtcg atgttgtcca
     4801 gcttgaagac ggtcgatcca tcagggttgc gtatctccga tgcgcgtttc acgaacttca
     4861 acccttcata gggactctgt ccggaacgag taaatcgccg ttctattctc acgatgcctc
     4921 ctccttggac gaggtaattg cagactgacg ttggctcacc ctacggaagt actagaccaa
     4981 tggcctcaac caatgctgct ttctatgcat ctttcgctca ttggagcata agtcctaaag
     5041 ctcatctgag gcgcggagtg actacatata gctattggag taaattgtca acactaaata
     5101 ttgtggtgta actgtggaaa cggaggaaat gctgtggata atagaaaggt cttgcctagg
     5161 gccggtccgc acaaaaaatt cgaaggttat ccacagttgt ggatagtgaa aacggaagaa
     5221 tttgggccgc gtgagatgta attaaacagg aactaaaacg ccaaacgacc cagtcgaacg
     5281 atcgcctcgt ctatgccctg caatggaata aaatgcccag caacgccgag cacgacaatc
     5341 cgggaattga acacggtgct gtcgtaaacg ctcacaagtc cccagttgcg tcggacggtg
     5401 tgcggacgta caacgctgtc aaccaactca tggctgtgcg aacgaaaaat cgtgcgcacc
     5461 atcgcccgct cgggcgagag ttgtgttccg tcgctgcgct caatggcctt ggcgagttca
     5521 ctctcgacaa aggtcagata ttcatcgatc gttggattgg aaagggcgag accgatgcat
     5581 acgctcagcg ccgtcaccag catcccgagc cggaacagag tcacggacat tcccctttga
     5641 caaacggtag tcgcatgatt agcctaccgg ccagtttgtt tcgacaatac aagtctaaca
     5701 tcgcgaacag ggcggagggc acattatgtt cgtatggagc aagaagctga tcgcatcgct
     5761 gctggcgttg ttcttggtgc tgttgggagt atttttgatt catggcgcgc ccgacagcgc
     5821 cagtgggttg aagattcaca caaccaggtg gatggcgctc aattacatcg gtctcatgat
     5881 ctgggtcttc gtctggatga tcgatcagac acgcatgagg ggtaaaaacg tgtggatctg
     5941 gctcatgcca tttgctctgg cacccttgcc gaccttaatg ttgttcgtac tcttccttca
     6001 gcgtcgtctt aagccggttt catgaccgga gattccggcg aagtttcgtc ggacgatata
     6061 tccggaggca gctggcgccg gtatagccag acgagccagc aaccaggcag cgccaacaca
     6121 aatgaaagaa aaaagaacca gccccacccc aacataccaa cggcttcagc ggaaggacga
     6181 ccggcaaaga cccgtccgag ggcttcgagt gaagacaaca acgcaaattg ggtggcagtg
     6241 tagcgatggt cgcagagcga catgaccaag gcgacaaagg ccgcggttcc catccctccg
     6301 gttacgtttt ctatgactac tgatgccgca agagcctcgt aactttttcc gatccacgcg
     6361 aggaccatga aggagaggtt cgagagagcc tggagcaggc cgaaaaacag aagcgaccga
     6421 atcaagcccg tcttggccat gatcagacca ccagccagtg ctccgattaa cgtcgctgcg
     6481 atgcccacgc ctttgacata ccctacttcg ccggtagaaa agccaaggcc gccgatgaaa
     6541 aaggccgtct gcaatgatcc ggcgacggcg tctccgagtt tatacaggac gatcaagcac
     6601 agcagtccat aaatgccagg gcgagcaaag agttccctta agggaaagcc gactgcctcc
     6661 ttgagcgttc tcggggcggc accctctccc ggaggatttg gactcagaag tatggtggcg
     6721 acgcctgccc ccatcaaggc agccagcacg aggtaggttc ctttccagcc aatctgctca
     6781 gcgaggacaa gggcgcccgc gctggccact aacatagcca gacgatagcc gttcacccat
     6841 aaggcagcgc cgaaccctcg ctccgcagga aggagcagat cggtgcgata ggcatcgaaa
     6901 acgatgtcca acgaagccga aaagaaggcg acgccaacag ccatcaatcc cagccaatgg
     6961 ggactctcat tgcttccaat gaatcccatc aaggccagac ccgacgccac agcgatttgc
     7021 gtgatcagca tccagcctcg ccgccgcccc aaccaaggca gcgagattcg gtcaattaag
     7081 ggagcccaga gaaattttaa gctgtacggc agacctacca gcgtgaagat tccgattgtt
     7141 ttcaggtcga cgccggcgac cgtcaaccag gcttgaaggg ttcccgaagt cagggccaat
     7201 ggcaaaccag atgcgaagcc caagggcagc atcagcgcga cgcgcttatt ggcaaggatg
     7261 tgtgcgaatg aatccgcggc catgtgatta tcgtgactcg tgtgggtcta gagtaacacc
     7321 ggaccgctga ctcgttgagg agaatctaga attctgtcgg agcaagcgat cgttcgaaac
     7381 cggaaagaaa gtgacaaccg ctcagcaagg ctgtccattg aaggtgcagg gtttgtagcg
     7441 agagacgtcg aattcgatgt tttcctggta gatgcgttca ttgccgttga cgccgtcttg
     7501 ctcgggatcg ttccacatgt tgtgattcca ccaataaaag agcgggtgga tttccgtttg
     7561 atacacgggg ttgccgaaat tactgcgggc gtattcctgg accatccggc cggtcacata
     7621 atcgacctga ccgtcgccct ttagcgaata cagcatgaga aacaatcgag cttcatggtc
     7681 gtacaactcg tcgatgcgcg tgcttagatc gggttcaatc ggtaggacgt ccttagacca
     7741 gacctgctcc tgaacaagga tgctcaagga aaacacgcaa caagcggtga atgagcggag
     7801 cgtcaccggt gtcctcatac ggcagggtcg tttacggcat gctagcacgg tgcttcgagg
     7861 ccttcaacca tgctgccatc ggtttgccgc acttgcccgc cgcgcaagca cgtctctata
     7921 atgatcgccc catgatcaag cgcgcgtcat tgcatagtct cggctgccgt ttaaaccaag
     7981 ccgaaacatc ggttttggcg tctcaattgc ggcgccaggg gtatgctctt gtcgactttg
     8041 gagagccgac cgatcttctt gtcgtcaata catgctccgt gaccgaggaa gcagaaagaa
     8101 cctgccgcta tgcgatacgg aaaaccttga aacattcgcc ggatgcgttc atagcggtga
     8161 ccggatgtta cgcgcaaacg ggtttgcaaa gcctgcggag cattcccggc atcgatctca
     8221 ttgtaggcaa tcagttcaag tcggacttgc cctcatttct gccgccgccg caaaccctgc
     8281 gtaaacaacc agcgccggaa gtgcttcaca ccaagaccat cgaccgagac gacttcgtcc
     8341 ttccagagtt cggagagcct cattcaacca gagctttggt caaaatccag gatggctgca
     8401 atgtgatgtg cagtttttgc ctcattccgt ttgcccgggg ccgcgagcga agccgcaagg
     8461 tcgaggacat cctttcggaa gccgccacac tggcggccgc tggccatcat gagcttgttc
     8521 tcacaggtgt gaacatcggg caattttctc aaagcggggc taccctcgcc gatttaattg
     8581 atcggttgga gaggatacat ggcatccaac gcatcaggct ttcgtc
//
LOCUS       DASTQY010000007         3746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_600567, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000007 DASTQY010000000
VERSION     DASTQY010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600567"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFR82_00170"
     CDS             <1..205
                     /locus_tag="VFR82_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500039.1"
                     /translation="IVLLLSWPDWALAEEAPHAGYRGIASIYYTLIWGILAYGLWDAF
                     GKKAFYVGGPVIAVIIYFLLPSS"
     gene            complement(483..2123)
                     /locus_tag="VFR82_00175"
     CDS             complement(483..2123)
                     /locus_tag="VFR82_00175"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU4500040.1"
                     /translation="MKAAELLVRCLENEGVELVFGLPGEETLELTDALLGSRTRFIET
                     RHEQGAAFMADVYGRLSGKAGVCLSTLGPGATNLLTGVADAFLDRAPLVAITGQASLN
                     RRHKESHQYIDVMSMFKPVTKWSASIPKAEVIPEAVRKAFKIAQTEKPGATHLELPED
                     VAEEQIGDSSLLQPLFVQAPVMPEPSPAQVAKAVRAISGAQRPVILAGNGVIRSRAHD
                     AVRQFARRVHIPVLHTFMAKGVFPDSDPLSLYTIGLLARDYASAVMEQADVVIAIGYD
                     FVEYAPCFWNPRRDKRIVHIDGSPAEVDEHYIVEVGLLGDLRRSLDLIGAGVPPFDLR
                     WSSISRKAVIDGFNTEVAGSPSWPLRPQHLMRELRAGLKPDDLVVCDVGAHKLWMARM
                     FPCEVPNSCIISNGFAAMGIAVPGAIAAKLLCPERRVVAVTGDGGFLMNSQELETAVR
                     LALPLVVLVWRDNGYGVIRWKQQLRFGRTSAVEFGNPDLLQYAGSFGAIGYRVTEPSE
                     LRPVLEEALNSKVPAVIDCPVDYAENLRLTERLQSLPH"
     gene            complement(2139..2792)
                     /locus_tag="VFR82_00180"
     CDS             complement(2139..2792)
                     /locus_tag="VFR82_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500041.1"
                     /translation="MLRGSALLTTMIVMAGCTSPGVSSPGHDVTAESYRTDTTKGLVV
                     LSALWGRSWKCGKFENGQLRSFGFDRLPSVTTADVATADVVIEDSTYVGGGPATNYVL
                     ALDPGEYGLAMFAIRVAFSTTDVRTVSVGRARLFEGGKPVGGTFTVQAGELVYIGHFG
                     LDCYKEPKIWRYYLEGRGGFEHYKRLIKNQYPFLPVETMQMRLFKTSALGRDYVLPP"
     gene            3161..>3746
                     /locus_tag="VFR82_00185"
     CDS             3161..>3746
                     /locus_tag="VFR82_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEU4500042.1"
                     /translation="MADQDLSEPAPDASSETRRKDLTDWVTWPDERLLDLRLCDLDVK
                     IAGSNLEDRIAQLYQELDGRGLRFHPHFWLSDEWFCPDDVPGIAIPFYLAHPRLAKLE
                     LAQMLEVEGGDPDSCMRILRHEAGHAIENAFKLRRRRHRQQLFGNSSEPYPDDYSPKP
                     YSKSFVLHLDSWYAQSHPDEDFAETFAVWLTPNSL"
BASE COUNT          788 a         1163 c         1042 g          753 t
ORIGIN      
        1 gattgtgctc ttgctgtcgt ggccggattg ggccttggct gaagaggccc cgcatgcagg
       61 ctaccgcggc attgcgtcta tctattacac gctgatctgg ggcatcctgg cgtacggctt
      121 gtgggatgcc ttcgggaaga aagcgttcta tgtaggggga ccggtgatcg ccgtcatcat
      181 ttactttcta ctcccgtcaa gctgacgggc gtcagcagcc ccaagacttc ttgggaagac
      241 ggcggcaagc tcaagcaggg cgtgccgccc atcccacgca aagatcatcc atgcatcgtc
      301 gctccacgtc gtgggctcga gttgagccaa ccggttcaag tccaaacgaa cgtcgtgatc
      361 cgcaacaatc cggagccacc ggctaatcct gaccgacttc ttcgctggag ataacgatca
      421 tgcgtcgctt tgcctgctgt catgatgagc aagaaagccg gcatctcacg gaaaatcagg
      481 agtcagtgcg ggagtgattg caggcgttct gtcagccgga gattctcggc atagtcaacg
      541 gggcagtcga tgacggccgg caccttgctg ttcaacgctt cctccaagac gggccgaagc
      601 tcagacggct ccgtgacgcg atagccgatc gcgccgaaac tcccagcgta ctgcagaagg
      661 tcagggttgc cgaattcaac ggcggacgtc cggccgaacc ggagctgttg cttccagcgg
      721 atgacaccgt agccgttgtc ccgccatacg agaacgacca gtggtaatgc cagacgcacg
      781 gccgtttcca attcctgaga gttcatcaag aacccaccat cgccggtgac cgccaccact
      841 ctccgttcgg gacacaacaa ctttgcggcg atggcgcctg gcacggcaat gcccatggcg
      901 gcaaacccgt tcgagatgat gcaggagttg ggcacctcgc agggaaacat ccgggccatc
      961 cagagcttat gcgcgcccac atcgcagacc accagatcat ccggcttcag acccgcgcgg
     1021 agttcccgca tcaggtgttg cggccgcagc ggccaggatg gtgagccggc cacttctgtg
     1081 ttgaacccat cgatcaccgc cttgcgtgaa atggaagacc agcgcaaatc aaacggcggg
     1141 accccggccc cgatgaggtc taaggagcga cgaaggtctc ccagcaggcc cacctcgacg
     1201 atgtagtgct cgtcgacctc ggcaggtgac ccgtcgatgt gcacgatgcg cttgtcacgc
     1261 cggggattcc agaagcaggg ggcatactcg acgaaatcat agccgatggc gatcaccaca
     1321 tcggcctgct ccattacggc cgacgcataa tcgcgggcca agagaccgat cgtatagagg
     1381 gacagcggat cgctgtcagg aaaaacccct ttggccatga aggtgtgcag gacgggaatg
     1441 tgcacccggc gcgcaaactg tctgaccgca tcgtgtgcgc ggctgcgaat cacgccgttt
     1501 cctgccagaa tgacaggacg ctgcgctccg gaaatcgcgc gcaccgcttt cgcgacctga
     1561 gccggcgacg gctccggcat gaccggcgcc tggacgaaca acggctgcag caggctcgaa
     1621 tcgccgatct gctcctccgc gacatcctcg ggcagctcca gatgtgtcgc accgggtttc
     1681 tccgtctggg cgatcttgaa cgctttccgg acggcttcgg gaattacctc cgctttcgga
     1741 atcgaggcgc tccatttggt gacgggcttg aacatcgaca tcacgtcgat gtattggtgc
     1801 gattccttat ggcgccggtt cagcgatgcc tgtcccgtga tggccaccaa cggcgctcga
     1861 tccaggaatg catccgcgac gccggtcaac agattggtcg cgccggggcc gagcgtggac
     1921 aggcacactc ccgccttccc gctgagcctg ccatagacat cggccataaa tgccgcaccc
     1981 tgctcgtgcc gggtctcgat gaagcgtgtg cgggaaccga gcagcgcatc cgttagctcg
     2041 agtgtttcct cgccgggcaa accgaagaca agttccacgc cttcgttctc caggcaacgg
     2101 accagcaact ctgccgcctt catgcaggga ctcctcgatt atggcggcag gacatagtcc
     2161 cgccccagtg cggatgtctt gaacaaacgc atctgcatgg tctccacagg cagaaatgga
     2221 tattggtttt tgatcaaacg cttgtagtgc tcgaatcctc cacgcccttc taaatagtag
     2281 cgccaaatct tcggctcttt atagcaatcc aatccgaagt gcccgatgta gaccagctca
     2341 cccgcctgta ccgtgaacgt cccgccgacc ggcttgccgc cttcaaagag ccttgcacgt
     2401 ccgacgctca cagtcctcac atccgtcgtg gagaatgcca cccggatggc aaacatcgcc
     2461 aaaccatact cgccgggatc caatgccagc acgtagttgg tggccggtcc ccctccgacg
     2521 tatgttgagt cttcgatgac cacatcggcc gtcgcaacgt ctgccgtggt cacactcggc
     2581 aaccgatcga agccaaatga tcgcaactgt ccgttctcga atttcccgca cttccacgag
     2641 cggccccaca acgctgagag cacgaccagg cccttggttg tatctgttct atacgactct
     2701 gcagtcacgt cgtgcccggg gcttgaaaca ccgggcgacg tacagccggc catcacgatc
     2761 atggtcgtga gcagcgcgga ccctctcagc atggcgcgtg gctcccaact cttgcaagcg
     2821 gttcagttga ccttcaccta tgacggaccc tacgctccca ccgggcgcac gtcaacaatg
     2881 gagagctgac tatctttcca tgaatgtaca aacgccttgg cattctgaac agcccatgct
     2941 catatgctta acgggagcat acacggcttc aaatgtgaag gtgataaggc cagccttatc
     3001 acgatttagc gagtcaggat cgccacatgc cagagcatag aaatccatta ttttccttcg
     3061 aacggctgaa atggcaaggc cggctggtgg ggatcgactg gacttcggtc aaaacgtaca
     3121 atgtcccggc ttcttgattc acactgtgtt gacctgaacg atggccgatc aagacctgag
     3181 cgaaccggca ccagatgcgt cctcggaaac cagacggaag gatctcaccg attgggtaac
     3241 gtggccggat gagaggttgc tggatcttcg gctgtgcgac cttgatgtca aaattgcggg
     3301 tagcaatctt gaggaccgca tcgcgcagct ttaccaagaa ctcgatggcc gcggccttcg
     3361 ctttcatccc catttttggc tatctgatga atggttctgt cctgatgatg tgcccggcat
     3421 cgcgatcccg ttttacctcg cccacccccg cctcgccaag ctcgaactcg ctcagatgtt
     3481 agaagtggaa gggggcgatc cggactcgtg catgcgcatc ctgcggcatg aggccggcca
     3541 tgccatcgag aatgccttca agctgcgacg ccgacggcat cggcagcagc ttttcggaaa
     3601 tagctccgag ccgtatcccg atgattattc tccaaagccc tatagcaaga gttttgtgct
     3661 tcatctcgac tcgtggtacg cccaaagcca tccagatgag gacttcgccg aaaccttcgc
     3721 cgtctggctg accccgaatt ctctct
//
LOCUS       DASTQY010000008        18779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1406904, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000008 DASTQY010000000
VERSION     DASTQY010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 18779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18779
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406904"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="VFR82_00190"
     CDS             complement(<1..1596)
                     /locus_tag="VFR82_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU4500043.1"
                     /translation="MERESSSSSLSEAPNLVHVLEEHRSLIAQRVMAGVSGAETLASM
                     TEFVDGLIIGRYREALRQGGEERATVGMRQCCLMALGGYGRRELAPQSDIDLMFLFQP
                     GADKAVESLVRAVLHPLWDCGFQVGHSVRTIADSTELAETDATVKTSMMEARFLAGSA
                     ELFQDFHRKYLRKVVFKNTDAYLDQKLEERRREYEKFGETVYLLEPNVKKSKGGLRDL
                     HLLQWAGMARYQAPTIRELSDRGILSRADLLALTEAREFLWRVRSLLHVHAGTAQEIL
                     TFDEQVWLAQHFGYHDQPHLLAVEQFMQQYYQHTMGLHERCMRFVERCRSVPFWRRVA
                     RFLPAPRVDGYFVVRGSLLSVADEHRDRVLDSPALLLHLFDLARDRNLTIVPPLIEDI
                     HRHVDQVSADAYRTPEVSRAFLRILSGPRTTGTLEAMHRAHLLEKLVPAMGTVRGLMQ
                     FNQYHKYTVDEHSLLAVGRAEALSQDTGVLGEVYRAIKRKDILHLAVLMHDLGKGREE
                     DHSDVGKRLSEEAALRLGLDEQET"
     gene            complement(1605..1943)
                     /locus_tag="VFR82_00195"
     CDS             complement(1605..1943)
                     /locus_tag="VFR82_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEU4500044.1"
                     /translation="MKLVEAIIKPFKLDEVKDALLEIGVQGMTVTEVKGFGRQKGHKE
                     TYRGQEYTIEFVPKVKIEVAVQDGQVPRVLETISRAAKTGSIGDGKIFVRDLSAAVRI
                     RTGETGETAL"
     gene            complement(1970..3241)
                     /locus_tag="VFR82_00200"
     CDS             complement(1970..3241)
                     /locus_tag="VFR82_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEU4500045.1"
                     /translation="MSERHHWVSRGAITGLWVTVCATQAWAGQREGSVDSGDVAWILA
                     ASALVVGMIIPGLAFYYGGLVRSKNVISTMVQAFAILCVVSVVWLVCGFTLVFGPDRG
                     GVIGGLDWALLMDVGAAPHPVYAPAVPLEAFMLFQLLFAAFTPALIAGAFAERIKFGA
                     MLLFAALWSVLVYTPIAHWLWGNGWLARIGALDFAGGAVVHISAGFSAVACAMVVGKR
                     RGWRTDYMAPHNLPFILLGAGLLWLGWFGFNGGSARGANATAVGAVTVTHFTAVSAAL
                     AWMLAEWKHRGKPTVLGVASGAVAGLATSTAGAGMLGPLAAVALGIAAGFCSYMAIVW
                     KGKIGYDDTLDVMGTHGVGGMLGMLGVGFFAAHGHPAMQILAVVATGTFSFLGSYAIL
                     KLVDGSIGLRVTQEEESTGLDLTQHNERAYS"
     gene            complement(3305..5074)
                     /locus_tag="VFR82_00205"
     CDS             complement(3305..5074)
                     /locus_tag="VFR82_00205"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEU4500046.1"
                     /translation="MRTLRIAMVQMNPTVGDLDGNVRRIINWLVEARKAKPDLVAFPE
                     LAITGYPPEDLLLKPQFVADNRRALNEIVRACRHCNVVIGYVGQGHASGLREGAAAVL
                     PAGRHDLYNAAAVITDCRLALTYAKWYLPNYGVFDESRYFSPGRRLPLITVNDTVVGV
                     NVCEDIWLSEGPTRLQANAGAEVIVNINASPFHGGKSRSREEMLATRARDNRVIVTYT
                     NTVGGQDELIFDGNSVVMDHTGEPIARAKAFEEDLLVVDLNVESVARARAAEGRRPEV
                     SGRTPTAIDRVTLKGKLPARRTRLIPSIEPLADPLDEVYRALTLGVRDYVAKNRFKRV
                     VIGLSGGIDSALTAVLAVDALGPRNVLGVFMPSPYTSRESCEDTQELARRLRIQVKTL
                     PITPTFKSYLQTLKPVFAGTKVDTTEENLQARIRGNLLMALSNKFGHLVLTTGNKSEM
                     SVGYATLYGDMAGGFAVIKDVPKTMVYALADRRNRQSPVIPKRTLDRPPTAELRADQK
                     DEDSLPPYPVLDPILKAYVEDDRSVDEMVGMGFARKTVLRVVSLVDRSEYKRRQAPVG
                     IKITHRGLGKDRRMPITNGYRGT"
     gene            complement(5474..6385)
                     /locus_tag="VFR82_00210"
     CDS             complement(5474..6385)
                     /locus_tag="VFR82_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEU4500047.1"
                     /translation="MTTIVKSAPASPAASSRSDSTGRTASIGRVGTLALEYVRRGGRT
                     IFGRTSCQTPWHLFPPIYLDDSGSAYTLLVNPSGGLVGGDRLSIDVSVGPKGHALIST
                     PSANRVYRSLSEESVQEIALKVGEGGIVEWIPEHTIPFAGSRFRQTIDVKLAAGATIV
                     LWDAMASGRIAHGERWKFTTFSNRIHIEPAAGSSLAEHYLIVPGNKWSGVGLAERWDY
                     VGSLFIVGDAITGTLRASLESALADIVEKQPDESILGGVSQPAASGLVVKLVSRSAPA
                     MALALAELWGAVRGLLWQLPPLALRKY"
     gene            complement(6369..7040)
                     /gene="ureG"
                     /locus_tag="VFR82_00215"
     CDS             complement(6369..7040)
                     /gene="ureG"
                     /locus_tag="VFR82_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEU4500048.1"
                     /translation="MHRADDQYCGSGRVSAARSKQVPVIGIGGPVGSGKTALVEALCR
                     TLRDRYSLAVVTNDIFTKEDAEFLTKRGALPEDRILGVETGGCPHTAIREDASHNQEA
                     LDELLRRHPEVELMFVESGGDNLAATFSPELVDHTIYVIDVAEGDKIPRKGGPGITRS
                     DLLVINKIDLAPYVGADLGVMDRDSRRMRGDRPFVFTNLMSGDGLDRVVTWVESHIHP
                     HDHHR"
     gene            complement(7117..7803)
                     /locus_tag="VFR82_00220"
     CDS             complement(7117..7803)
                     /locus_tag="VFR82_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEU4500049.1"
                     /translation="MNTLSLIQGLRFVDSFFPSGGFAFSSGLEAAVKCGAVHSGKDLA
                     GYVEDLLVHGIAKREAAGVAAGHLAATTDRLDLALAVDRQLDELKLGRDSRLASRQIG
                     RQVMKGSAGQTDENRVFREFRRAIDEGRTPGHVPVAIGITLAVCGWARQDTVAAFLYQ
                     TVQGFVSAAMKLLPVGQQEGQQLLHDWGPLITELSRRAKPQTSLVSWSPVHDIYAMRH
                     ARLESRLFRS"
     gene            complement(7845..9572)
                     /gene="ureC"
                     /locus_tag="VFR82_00225"
     CDS             complement(7845..9572)
                     /gene="ureC"
                     /locus_tag="VFR82_00225"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEU4500050.1"
                     /translation="MKIPRKQYADLFGPTVGDRVRLADTDLLVEIEKDYTSYGDEAVF
                     GGGKVLRDGMGQSARVTNADGALDTVITNALILDYWGIVKADIGIKNGRIVGIGKAGN
                     PDTMPNVSPGMACGAGTEAISAEGCIVTAGALETHVHFICPQQCWEALSAGITTMIGG
                     GTGPATGTNAVTSTPGPWNIHRMLEAAEGIPINLGFTGKGNCSLPGPLNEQIEAGAIG
                     LKVHEDWGSTPAAIDSALSVAERYDVQVALHTDTLNEAGFVEDTIKAFKGRTIHSYHT
                     EGAGGGHAPDVIKLCGEPNVLPSSTNPTMPFTVNTLDEHLDMLIVCHHLNKRVPEDVA
                     FAESRIRRETIAAEDVLHDMGAISMMSSDTQAMGRIGELIIRTWQTAHKMKLQRGHLT
                     ERGAQSGKGQNDNFRAKRYIAKYTINPALAHGVAHEVGSVEVGKVADLVLWKPAFFGI
                     KPEMVLKSGFIAQAQMGDPNASIPTPEPIISRPMFAAFGRAVASTCLTFVSAAAVERD
                     VPHQLGLQRRIVAVKGCRTVKKRDLKMNDALPQVEVDPETYIVTADGERLTCEPIIVQ
                     PMAQRYFLF"
     gene            complement(9569..10042)
                     /locus_tag="VFR82_00230"
     CDS             complement(9569..10042)
                     /locus_tag="VFR82_00230"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009682.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEU4500051.1"
                     /translation="MSKTKNRLAGKAAKKSASSLAGAIKAGLAKPVIPGEILFAAGEI
                     EAFKGRATKDLTVKNTADRPIQVGSHCHFFEANRGLLFDREQAFGYRLCIPAGTAARF
                     EPGEEKRVTLVALGGARTVQGINGLTNGALDDAQAKARALARAAEHGFIRKGGGR"
     gene            complement(10070..10372)
                     /gene="ureA"
                     /locus_tag="VFR82_00235"
     CDS             complement(10070..10372)
                     /gene="ureA"
                     /locus_tag="VFR82_00235"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEU4500052.1"
                     /translation="MHLTPREQEKLLIYTAGQLAADRMKRGLKLNHPEAVAYLTAAVL
                     EGIRDGKTVSELMTYGTTLLSYDDVMPGVPEMIHEIMVEGTFPDGTKLVTVHNPIR"
     gene            10493..11545
                     /locus_tag="VFR82_00240"
     CDS             10493..11545
                     /locus_tag="VFR82_00240"
                     /inference="COORDINATES: protein motif:HMM:NF015226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HEU4500053.1"
                     /translation="MARVALCDRCREHPVWGFGDWVLRGIAQVVFQNNPITGALILAG
                     IFYNSWIYGLACLFGTIISTATAVLFKADKGLIKDGLFGFNGALVAVALVAYTSPDFT
                     TGNMPNLHLWLYIVLCAVFTSVILPAFGALLGPHRVPALTMPFVLATWFFLGALLQFT
                     TIDVSNAIKPTSPADFTGPRPEYSWLTWFHGMTMGISEIFFQDNWVTGIIILLAIAVN
                     TRLGALMALMGTTLAVGSAIFYGAHEEVIRDGLFGYNAALTAMALGGLFYVLNLPCLV
                     YTVIGILVTARVWASLGIFLEPTGMPVLTSAFVFVTWLMLLAKPGFSRSLIPVAPGDA
                     TTPEENLARHRASQKR"
     gene            complement(11533..12762)
                     /locus_tag="VFR82_00245"
     CDS             complement(11533..12762)
                     /locus_tag="VFR82_00245"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4500054.1"
                     /translation="MLALGSLLADAWAEDTPGPVAQRLEHVALRLKEGEKAVEKRLLS
                     ESFRLHFYGYLEGSYTQNFNNPSNRINQLRIFDVNSNQFRPNVAQFVLEREVKAEATG
                     WDRIGFRVKFNVGRDSDFIGGTNLSTWADFQEVYGQYLAPVGRGLNLQAGQFNSVVGY
                     EVVESPRNAHYSRSWLFGLGQPFTTRGIRATYEFNTLVSLSVGAIAYINSARADTNFD
                     PLVEYALSVTPTDKVRLTLYGLAGPRDGPSATPGGTVFLAGGYLSVQITDHTSTVIES
                     YYANQSNSSTISTSRNARWDGAAVYLIHDFTDQWGLRLRGEIFEDAGGFVACQGTTDY
                     QPRANVCFGATSGTSAPAVAQTLWEFTGTVQYKPFASFITRLEYRYDKSNQNVFQVGG
                     RATSYQPTLSLDVIYLF"
     gene            complement(12926..13498)
                     /locus_tag="VFR82_00250"
     CDS             complement(12926..13498)
                     /locus_tag="VFR82_00250"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEU4500055.1"
                     /translation="MKRHASRKRRFIATLAAMTLMLTGWLSGPASAGSARQIDAGVDD
                     AIAKFEQEVKGGKSFMESSKGMLVFPTVLKGGAGFGGEYGEGALRIGGKTADYYSMLQ
                     GSFGFQLGGEIKTVYLVFLDEGALKRFRDSEGWKAGVDGSVSLVTLGAGGTIDTNNLK
                     DPIVAFVLGQKGLMYNLSLEGTKFTKQNKR"
     gene            complement(13693..14628)
                     /locus_tag="VFR82_00255"
     CDS             complement(13693..14628)
                     /locus_tag="VFR82_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEU4500056.1"
                     /translation="MKTLLLVILALVGVVAHAAAWGDLGHMMAGVIAYEQLTPETRQK
                     VTTLLKLNPKHATWVANVPEDEKPRIAFLRASRWADDIKRDPAYEQDGSEGGNRPAGL
                     NAARNIGYTDTYMHKYWHFVDLPFSPDNVMFTDPPTPNAQTQIALFRQALRSTASSDS
                     LKSYDLVWLIHLVADVHQPLHAVSRFDKAHMNGDDGGNGVMVCSHPCEIRLKLHAFWD
                     NVLGVSTETTTAIETAKQLPAADPELAAISDERIWIQESFEAARTHVYVSPVEIGVGP
                     FELTDGYKEAAQKVAAQRIGLAGARLANLLNDALR"
     gene            complement(14700..15410)
                     /gene="urtE"
                     /locus_tag="VFR82_00260"
     CDS             complement(14700..15410)
                     /gene="urtE"
                     /locus_tag="VFR82_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HEU4500057.1"
                     /translation="MLELKQINAYYGESHILRDVTFTVSDGEVVCLMGRNGVGKTTTL
                     KVITGLLSARSGDMVFNGSNITKLSTDRRARRGLAIVPQGREIIPHLTVRENLLLGFW
                     ARSDRQNGATEKAAFDEVYQLFPKLTQILHRPGGVLSGGEQQQLAIGRALLSNPKLLL
                     LDEPTEGIQPSIVDQIEDVILGFKKSRRFAILLVEQGLHFAARLAEKYVVMAKGAVVA
                     AGKSDELSAEMVRQHLTV"
     gene            complement(15492..16259)
                     /gene="urtD"
                     /locus_tag="VFR82_00265"
     CDS             complement(15492..16259)
                     /gene="urtD"
                     /locus_tag="VFR82_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEU4500058.1"
                     /translation="MSDRGSIIYLEGVVVDYDGFKALNNLNFIVDYNELRVVIGPNGA
                     GKTTLLDVICGKTRPVAGRVIFGKDQELIGKREDEIVKLGIGRKFQAPSIYENLTVWE
                     NLDLSLKRPSKGVFSTLLGTSTAEEREKIAETLMTIGLGDYAGMRAGSLSHGQKQWLE
                     IGMVILQDPSLLLVDEPVAGMTDKETEQTGRLLQSLAEKHAIVVIEHDMEFVRQIARI
                     VTVLHEGTVICEGTVEKVQSDDRVREIYLGRQKVAHG"
     gene            complement(16256..17395)
                     /gene="urtC"
                     /locus_tag="VFR82_00270"
     CDS             complement(16256..17395)
                     /gene="urtC"
                     /locus_tag="VFR82_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEU4500059.1"
                     /translation="MPSHDHLPSASRADVGLAFWVAGFVLLILMPVLNVMPAEDSWFH
                     LSDFSLNRFGKFLAFAILALGLDLIWGYTGVLSLGQGVFFGLGAYCMGMHLMLTIGKE
                     SVYGSELPDFMVWNQVKALPLFWKPFYSFPVAILGAILVPTAFALVFGFLAFRSRIRG
                     VYFAIITQALALVAWLVFNRNETNVGGTNGLTDFKQLLGFRLSDPGTQRALYVLTVLC
                     LGGAYLLCLWITRSRAGRVLIAVRDSEPRVVFSGYTPANYKLFVFVVAAALAGLAGML
                     YVPQVGIITPAQIGVLPSLDMVIWVAVGGRGTLAGAVLGAVSVNLGRSLLTNYFPELW
                     PFILGGLFVVVVLLFPDGLVGIMRHLKERFQNMRATALSAEEGLR"
     gene            complement(17417..>18779)
                     /gene="urtB"
                     /locus_tag="VFR82_00275"
     CDS             complement(17417..>18779)
                     /gene="urtB"
                     /locus_tag="VFR82_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEU4500060.1"
                     /translation="LGDSTLIPRLDDIRANADRPVRLAIKPVMDLLRNRAKLSSPDPD
                     HRRSAATDLGTSGKTVAIPWLDHAAATESNKWVRYTMQESAALLHLAGDDHAAKVSAV
                     RTLGDLASQNAVPALQQFVTLGSAAEATEAQQALAKVASASIERIETWAAWSSAIETI
                     FRGVSLSSILLIMSVGLAIVFGLMGVINMAHGELMMVGAYATFITQELFKAFLPTAMF
                     DYYFAAAMPMAFLVAAACGMLLEVTVIRFLYGRPLETMLATWGISLVLMQAARVYFGD
                     LTAVVAPSWLNGGVQVMVGVYLPNNRLFIIALSFLCVLAIYAMLFRSGIGLRVRAVMQ
                     NRNMSACLGIPTRKVDAYTFAFGSGLAGVAGWALTLIGNVEPGLGQNYIVDSFMVVVT
                     GGVGKLAGTIVASLGIGGLNKLLEPSLGAVYGKVCILMLVILFLQWRPSGLFAIKGRH
                     ADV"
BASE COUNT         3930 a         5773 c         5145 g         3931 t
ORIGIN      
        1 ggtttcctgc tcatcgagtc caagacgaag cgccgcctcc tcggacaacc gcttgcccac
       61 gtcgctgtgg tcctcctcac ggcccttgcc cagatcgtgc atcaagaccg cgaggtggag
      121 aatgtctttg cgcttgatcg ctcgatacac ctctcctaat acgccggtat cctgactcaa
      181 cgcttcagcc cggccgacgg ccagcagact gtgctcgtct acggtgtact tatggtactg
      241 attgaactgc atgaggcccc gtaccgtccc catcgccggc accagctttt cgagcagatg
      301 ggcccgatgc atggcttcca acgtgccggt cgtgcgcggc ccggacagga tccggaggaa
      361 cgcgcggctg acttcgggcg tccggtacgc gtccgcagac acctgatcga cgtggcggtg
      421 gatgtcctca attaacggag gcacgatggt cagattcctg tcccgggcga gatcgaacag
      481 gtgaagcaga agcgccgggc tgtccaacac cctgtcgcgg tgctcgtccg caaccgagag
      541 caggcttccc cgaacgacaa agtaaccatc cactcgcggg gccggaagga agcgcgccac
      601 ccgccgccaa aacgggacgc ttcggcatcg ctcgacgaat cgcatgcagc gttcgtgcaa
      661 tcccatggtg tgctggtaat actgttgcat aaactgctcg accgcgagca gatgtggttg
      721 atcgtgatag ccgaagtgct gcgccagcca cacctgctcg tcaaacgtga ggatttcctg
      781 cgccgttccg gcatggacgt gaagcagtga tcgaacgcgc cacaagaatt cccgtgcctc
      841 ggtcagcgcg agcaaatccg ctcgggagag gatgccgcga tccgagagtt cgcgaatggt
      901 cggggcctga tagcgtgcca tgccggccca ttgcaggagg tggagatccc gaagaccgcc
      961 cttgcttttt ttgacgtttg gctccagcag atacaccgtt tcaccgaact tctcgtattc
     1021 ccggcgccgt tcctcaagtt tctgatccag ataggcatcg gtattcttga agacgacttt
     1081 gcggagatac ttgcgatgaa agtcctggaa cagctctgca ctccccgcga gaaaccgcgc
     1141 ttccatcatg gaagtcttga cggtcgcatc cgtttcagcc aattcggtgc tgtcggcaat
     1201 ggtcctgacg ctgtgaccga cctgaaatcc gcaatcccac aatgggtgca ggactgcgcg
     1261 aaccagggat tccaccgcct tgtccgcgcc cggctggaac aggaacatca aatcgatatc
     1321 ggattgcggg gccaattccc ttctgccata gccgcccaat gccatgagac agcattggcg
     1381 catgccgacg gtcgccctct cttcgccgcc ctgccgcaag gcttcgcgat agcgtccaat
     1441 gatcaacccg tcgacgaatt ccgtcatcga cgccagggtt tctgcacccg agactcctgc
     1501 catgacgcgt tgtgcgatga gagaccggtg ctcttcaagc acgtggacaa ggttgggggc
     1561 ttccgaaagg gaggacgaag aagattcgcg ttccatgatg ggtgttacag ggccgtctcc
     1621 cctgtttctc cggtgcggat gcggacggcg gcgctcagat cgcgcacaaa gatcttcccg
     1681 tctccgatgc tcccggtctt ggccgcccgt gaaatcgttt ccaagacccg tggcacctgt
     1741 ccatcctgca cggccacctc gatcttcacc ttggggacga actcgatcgt atattcctgg
     1801 ccgcggtagg tttctttgtg ccctttttgg cggccgaatc ctttgacctc cgtcaccgtc
     1861 atcccctgca caccaatctc caacagcgcg tccttcactt catcaagttt gaacggcttg
     1921 atgatggctt caacgagttt cataagcggt ctccatgctc agcattccgt cacgagtagg
     1981 cacgttcgtt atgttgtgtg agatcaagcc ccgtcgattc ctcttcctgg gtcacgcgca
     2041 acccgatcga tccgtcgaca agttttaaga tggcgtaact gcctagaaaa gaaaatgtgc
     2101 cggtagccac gacggcaaga atctgcatcg cgggatggcc gtgtgccgcg aagaatccga
     2161 cgcccagcat gccgagcatg ccgcccacac catgcgttcc catgacgtcg agcgtgtcat
     2221 catagccgat cttccccttc caaacaattg ccatatagga gcaaaaaccc gcggcaatgc
     2281 cgagcgcaac agcggccagc ggcccgagca ttccggcacc ggcggtcgag gtcgccaagc
     2341 cggcaaccgc gccgctggcg actcccaaca cggtcggctt tccccggtgt ttccattccg
     2401 ccagcatcca cgcaagggcg gcggagacgg cggtgaagtg cgtcacggtc acggccccga
     2461 ccgccgtggc attcgccccc cgggcgctgc cgccgttgaa accgaaccag ccgagccaca
     2521 ataaaccggc gcccaacaaa ataaagggta gattatgggg cgccatgtaa tcggtgcgcc
     2581 agccgcgccg tttgccgacc accatcgcac aagcgaccgc gctgaacccc gcgctgatat
     2641 ggacaacggc gccgccggca aaatcgagcg caccgattct cgccagccac ccgtttcccc
     2701 atagccaatg ggcgatgggc gtatagacca ggacggacca gagagcagcg aacagcaaca
     2761 tcgcgccgaa cttgatccgt tcggcaaacg ctccggcgat caaggcgggg gtgaaggccg
     2821 caaagagcag ctgaaagagc atgaacgcct cgagcggaac ggccggcgca tacacaggat
     2881 ggggcgcagc tccgacgtcc atgagcaatg cccaatccaa tccaccaatc acacctcccc
     2941 ggtccgggcc aaacaccagc gtgaagccac agaccagcca gacgacgctg acgacgcaga
     3001 gaatcgcgaa cgcctggacc atcgtgctga tgacgttctt actgcgaacc aggccgccat
     3061 aataaaaggc caggccgggg atgatcatgc ccacgaccag tgccgatgca gccagtatcc
     3121 aggcgacatc gccgctgtcc acgctgcctt cacgttggcc ggcccaggcc tgcgtggcac
     3181 aaacggtcac ccacaagcct gtgatcgcgc ctcgtgaaac ccagtgatgc cgttcactca
     3241 tgccgcctcc tctctaaaac catgatgcga aacaagttgt ctgctgtgcc cgcacctcac
     3301 gaaactacgt gcctcggtat ccgttcgtga tcggcatgcg tcgatctttt cccagccccc
     3361 gatgcgtaat cttgatgccc acaggtgcct ggcgtcgctt gtattcactt cgatccacca
     3421 gactcaccac ccgcagcacg gtctttcggg cgaatcccat cccgaccatc tcatccacgg
     3481 accggtcatc ctcgacgtag gccttcaaaa tcgggtcgag taccggataa ggcggcagac
     3541 tgtcttcgtc tttttgatcc gctcgtaact cggcagtcgg cggcctatcc agagttcgct
     3601 ttggaatgac cgggctctgc cggttgcggc ggtcggccaa tgcatacacc atagtcttgg
     3661 gcacgtcttt gatcaccgca aaccctccgg ccatatcgcc gtacagggtg gcgtagccga
     3721 cgctcatttc gcttttgttg cccgtcgtca acacgagatg tccaaacttg ttcgataggg
     3781 ccatcaacaa attgccccgg atccgcgcct gcagattctc ttcggtcgta tcgaccttgg
     3841 tgccagcgaa tacaggtttc agcgtctgca gatacgactt gaaggtcggc gtaatcggca
     3901 gcgtcttgac ttgaatccgg aggcggcgcg ccaactcttg ggtatcctcg cagctctcgc
     3961 gagaggtata gggtgacggc atgaacacgc ccagcacatt ccggggaccg agcgcgtcca
     4021 ccgccagtac agcagtcagc gccgaatcga ttcccccgct caggccaatg acgactcgct
     4081 tgaagcggtt ctttgccacg taatcacgca ctcccagcgt caaggctcga tagacttcat
     4141 ccaacggatc ggccaacggt tcgatcgatg ggatcaatcg tgtccgtctg gcaggcagct
     4201 ttcccttgag ggtaacccga tcgatggccg tgggcgtcct tccgcttacc tccggccttc
     4261 tcccctccgc cgctcgtgct ctggcaaccg actcgacgtt cagatcgacg accaataagt
     4321 cctcttcaaa ggccttcgct ctggcaatcg gctcgccggt atgatccatg acgacgctat
     4381 tgccgtcaaa aatcaactcg tcctgtccgc cgacggtgtt cgtatacgtg acgatcaccc
     4441 ggttgtcccg ggcacgcgtc gccagcattt cttcgcgcga ccggctcttg cccccgtgaa
     4501 acggcgaagc attgatgtta acgatcactt ctgctccggc attggcctgc agtctcgtcg
     4561 gcccttcgga aagccagatg tcttcgcaga cgttcacgcc aacgaccgtg tcgtttaccg
     4621 tgatgagtgg gagacggcgt cctggactaa agtaccggct ttcgtcgaag acgccataat
     4681 tcggcaaata ccatttggcg taggtcaagg ccaggcggca gtcggtgatg acggctgcgg
     4741 cgttgtacaa gtcgtgtcgc ccggcaggaa gcaccgccgc ggctccctct ctcagccctg
     4801 acgcgtgccc ctgtcccaca tagccgatga ccacattgca atgtcggcac gcacgaacaa
     4861 tctcgttcag cgcacggcga ttgtccgcaa cgaactgcgg cttcaacagg agatcttcag
     4921 gtggataccc ggtgatggcc aattcgggaa acgcgaccag gtccggcttg gcctttcttg
     4981 cttccaccag ccagttgatg atccggcgca cattgccgtc cagatcccca acggtgggat
     5041 tcatttggac catggcaatg cgaagggtgc gcatcaaacc tatctgtctc cagtcggcac
     5101 agccgcagaa tgcccaaagc ggctgcccgg caaggccaca acgaagctca tacgccgagg
     5161 aggtatatac cgagcttcgc gtgagccgct cgcttcgatc aaatgcgagc ggataggtat
     5221 tttgcctcca gtggacgaaa tcgtacgtcg agtgtccgag cgagtgagaa cgcatctggc
     5281 ggtcgctttg agcattctgc ctaaaaaaaa acgccctcgg atcccttggc aggaccgagg
     5341 acgtcattgt ccgtcattct gtcgatgtga tgtggagact ccattgtctc cgaaggtcga
     5401 gcggaggata gcacccggct cgatggagtg tcaaaccgtg cacgacgaat ccaagacaca
     5461 acgggcccgc tgctcaatat ttcctcaagg ccagaggcgg cagttgccac agtagacccc
     5521 gtacggcacc ccacaactca gccaatgcca gagccatcgc cggcgccgat cgtgatacga
     5581 gtttgaccac gaggcctgac gcggcgggct gggacacgcc accgagtatg gattcatctg
     5641 gttgcttctc cactatgtcg gcaagagccg attccagcga tgccctcagg gttccggtga
     5701 tcgcatcccc gacgataaac agcgatccga cgtaatccca acgttcagcc aggccgactc
     5761 cgctccattt gtttccggga acgatgaggt aatgctccgc gagtgatgag cctgccgctg
     5821 gttcgatgtg aatccggttg ctgaacgtcg tgaacttcca acgttctccg tgggcgatcc
     5881 ggccggaagc catcgcatcc cacaacacga tggtggctcc ggctgccagc ttcacatcga
     5941 tcgtttgacg gaaccgggaa ccggcaaaag gaatcgtatg ctccggtatc cactcgacga
     6001 ttcctccttc ccccactttg agagcgatct cttggactga ctcctctgac agggatcgat
     6061 acacacgatt ggccgacggg gtcgagatga gcgcatgtcc ctttggacca accgagacat
     6121 cgatggaaag ccggtcgcca ccgaccaggc caccagatgg attgaccagc agtgtgtaag
     6181 cactgcctga gtcatccaga tagataggag gaaagagatg ccagggcgtc tggcagcttg
     6241 tccgtccgaa tatcgtgcga ccgccccgcc ggacgtattc cagtgccaat gtgccgactc
     6301 gaccgattga tgccgtccgg cccgtggaat ccgagcgaga cgacgccgca ggggaggccg
     6361 gggcgctttt aacgatggtg gtcatgcgga tggatgtgcg actcgaccca ggtcacgacc
     6421 cgatccaggc cgtcgccgga catgaggttt gtgaacacaa acggccggtc gccgcgcatg
     6481 cggcggctgt cgcgatccat cacgccgaga tcggcgccga cgtagggtgc gagatcgatc
     6541 ttgttgatca ccagcaggtc ggacctggtg atgcccggcc ctcccttgcg ggggatcttg
     6601 tcaccctctg ccacgtcgat gacgtaaatc gtatggtcca ccagctccgg gctgaacgtg
     6661 gcggccagat tgtcgccgcc gctttcgacg aacatgagct cgacctcagg atgtctgcgc
     6721 aatagttcgt cgagcgcctc ttgattgtgg gaggcatctt ctcgaatcgc cgtatggggg
     6781 cagccgccgg tctcgactcc caggatgcgg tcttcgggca atgcgcccct tttcgtgaga
     6841 aattcggcgt cttctttcgt gaaaatatcg ttcgtgacga ccgccagact gtatcgatcc
     6901 cgcaatgttc ggcagagcgc ttccaccagc gcagtcttgc cggatccgac cggcccgccg
     6961 attccaatca cgggaacctg ctttgagcgg gctgcgctga ctcggccgct tccacaatac
     7021 tgatcatcgg ctcgatgcat tgagacctca aaaggcgtcc ggcctacttg tttggcaact
     7081 acagggatat gccgggacga gctgatcgtt cgacgttcag gaccgaaaca accgtgattc
     7141 caaccgtgcg tgccgcatcg cataaatgtc gtgcaccgga gaccacgaca ctaagcttgt
     7201 ctgcggtttc gcccgacgac tgagttcggt aatcaacggc ccccaatcat ggaggagctg
     7261 ttggccttcc tgttgaccta ccggcaacag cttcatcgcc gcggacacaa atccctgtac
     7321 tgtctggtag agaaatgccg caaccgtgtc ctgcctcgcc cacccgcaga ccgccaaggt
     7381 tataccgatc gccacaggga cgtggccggg tgtccgccct tcgtcgatgg cacgacgaaa
     7441 ctctctgaac accctgttct catcagtctg cccggcagac cctttcatga cttggcgccc
     7501 aatttgtcga cttgccaagc gtgagtcacg acccagcttc agttcatcca actgacgatc
     7561 gaccgccagt gccagatcaa gcctgtctgt cgtggcagca agatggccgg ccgccacacc
     7621 agccgcctca cgtttcgcga taccgtgcac cagcagatcc tcgacatacc ctgcgagatc
     7681 cttcccacta tggacggccc cgcatttgac cgccgcttcc agaccggagg aaaaggcaaa
     7741 acccccagac gggaaaaaac tatcaacgaa gcgcaaccct tggatgagag acagcgtgtt
     7801 catagcgtct cgttcaacta agtcgagtgg cgacctttat cagcctaaaa caggaaatac
     7861 cgttgcgcca tcggttgcac aatgatgggc tcacaggtca gtcgctcccc gtcagccgtc
     7921 acgatatacg tttcagggtc gacctcgacc tggggcaagg catcgttcat tttgaggtcg
     7981 cgctttttaa cggtgcggca ccctttgacc gccacgatcc gtcgctgcaa gccgagctga
     8041 tgaggaacat ctcgctctac tgcggcggca gacacgaacg tgaggcaggt gctcgcaacc
     8101 gcgcgtccga aggcagcgaa catcggcctg ctgatgatcg gttcaggagt gggaattgag
     8161 gcattgggat cgcccatctg cgcctgcgcg atgaaaccgc tctttaagac catttccggc
     8221 ttgatgccga agaacgccgg cttccatagg accaaatccg cgaccttgcc gacttcaacc
     8281 gaacccactt catgcgccac gccgtgggcc agagccggat tgattgtgta cttcgcgata
     8341 taccgctttg cacggaaatt gtcgttctgc cctttgccgg actgcgcgcc gcgctcggtc
     8401 aaatggccac gctgcagttt catcttatgg gccgtttgcc atgtgcggat gatcagctcg
     8461 ccgatccgtc ccatcgcctg ggtgtcggag gacatcatac tgatggcgcc catgtcatga
     8521 aggacgtctt ccgcagcaat cgtttcacga cgtatgcgtg attcggcaaa cgccacgtct
     8581 tccggcacgc gtttattgag atgatggcag acgatgagca tgtcgagatg ctcatccaac
     8641 gtgttgaccg tgaacggcat ggtcggattg gtcgaggacg ggagcacgtt cggttcgccg
     8701 cacaacttga tgacgtcggg cgcatggccg ccgccagccc cttcggtgtg gtaggagtgg
     8761 atcgtccggc ctttgaacgc cttgatcgtg tcttccacga agcccgcttc attcaacgtg
     8821 tccgtatgca aggcgacctg gacgtcgtag cgttccgcca cgctcagcgc actgtcgatg
     8881 gccgcaggag ttgaacccca gtcttcgtgt actttcaatc cgatggctcc ggcctcgatc
     8941 tgttcgttca aaggccctgg cagcgaacaa ttgcccttgc cggtgaaccc caaattgatc
     9001 gggatccctt cggctgcttc caacatgcga tggatgttcc atgggcccgg tgtagaagtc
     9061 actgcgttcg tgcccgtcgc cgggccggtc cctcctccga tcatcgttgt gatgccggcc
     9121 gagagcgctt cccaacattg ctgtgggcag atgaaatgta cgtgtgtctc gagtgctcct
     9181 gccgtgacga tgcagccttc cgccgagatg gcctcggtgc cggccccgca ggccatgccg
     9241 ggcgacacgt tgggcatcgt atcgggattt cctgccttgc cgatccccac gatacggccg
     9301 ttcttgatgc cgatatccgc cttgacgatg ccccaataat cgaggatgag tgcattggtg
     9361 atcacggtat ccagggcgcc gtcggcattg gtcacacggg ccgactgccc catgccgtcg
     9421 cgaagaactt tccctccccc gaacaccgct tcgtccccgt aggaagtgta gtctttttcg
     9481 atttcaacca gcagatcagt atcggctagg cggacgcgat caccgacagt gggaccgaag
     9541 agatccgcat actgcttgcg gggaatcttc accggccgcc tcctttcctg atgaatccat
     9601 gttcggcggc tctcgccaag gctcgggcct tggcctgcgc atcatcgaga gctccattgg
     9661 tcaacccatt gatcccctgc accgtgcgcg caccgcccag tgcgacaagc gtcacccgtt
     9721 tctcttcgcc cggctcgaaa cgagccgccg ttccagcagg gatgcaaagc ctgtagccga
     9781 acgcctgctc gcgatcaaag aggaggcccc ggttcgcttc aaaaaaatgg caatgcgatc
     9841 caacctgaat cgggcggtcg gccgtgttct tgaccgtgag gtccttggtc gcgcggcctt
     9901 tgaaggcttc gatctcaccg gcagcgaaca ggatctcgcc aggaattacc ggcttcgcca
     9961 gtccggcttt gatcgctcct gcaagcgaac ttgcagattt ctttgcagcc ttgcctgcga
    10021 ggcggttttt cgtttttgac attctgtgtc ctcccgacga gagggaatct cagcgaatgg
    10081 gattgtgcac ggtgacaagc ttggtgccgt ccggaaacgt cccctcgacc atgatttcat
    10141 ggatcatctc cggcacgccc ggcatgacgt cgtcgtacga caagagggtg gtcccgtagg
    10201 tcatcagttc cgacaccgtc ttgccgtcgc gaatgccttc aagaaccgct gctgtcaggt
    10261 aggcaacggc ttcgggatgg ttcagcttga gaccccgctt catgcgatcc gccgcgagtt
    10321 gtcccgcggt atagatcagc agtttttcct gttctcgtgg agtcaaatgc atcgaggtcc
    10381 ccccattttt tacctaggta cgccggcaga attttgttgc ggggaatcgg atgctagtct
    10441 acgattcgct gcctgtcaag cacgctcggt ctgcatacga aaggagagcg ccgtggcgcg
    10501 agtggccctg tgcgatcggt gtcgtgaaca tccagtgtgg ggattcggcg actgggtgct
    10561 gcgtggaatt gcccaggtgg tctttcagaa taacccgatc accggtgcgc tgattctggc
    10621 aggcatcttc tataattcct ggatctacgg cctcgcctgt ctcttcggca ccatcatcag
    10681 cacagccacc gccgtcctgt tcaaagcaga caagggtctc attaaggacg gtcttttcgg
    10741 ctttaatggc gcattagtcg ccgtcgccct cgtggcctat acgagccccg atttcacaac
    10801 cgggaatatg cccaacctgc atctctggct gtacattgtg ctctgcgctg tcttcacgtc
    10861 ggtgatcctt ccggcgtttg gcgcgttgct gggaccgcac agagtcccgg ctttgaccat
    10921 gccttttgtc ctggcgacat ggttcttcct cggcgccttg ctacaattca ccacgatcga
    10981 cgtctcgaac gcgatcaaac ccacttcacc agccgacttt acaggccccc gaccggagta
    11041 cagctggctc acctggtttc acggaatgac aatgggcatt tcagagatct tcttccagga
    11101 caattgggtg accggaatca tcatcctcct cgccatcgcc gtcaacacac gtctcggcgc
    11161 cctcatggcc ttgatgggaa cgaccctcgc agtaggaagc gcgatctttt atggggccca
    11221 tgaggaagtg atccgcgacg gactgttcgg ctataacgct gcgctcaccg ccatggcgct
    11281 tggaggactc ttctacgtgc tgaatctacc gtgcctcgtg tatacagtca tcggcatcct
    11341 cgtcaccgcg cgtgtgtggg catcgctggg tatatttctt gagcccaccg gcatgcctgt
    11401 gctgacgtcg gcctttgtgt tcgtcacctg gttgatgctc ttggccaaac cgggtttcag
    11461 cagatctttg attccggtgg cgccgggcga tgcgaccacg ccggaagaga acctcgcccg
    11521 ccatagagca agtcagaaga gatagatcac atccagagac aaagtcggct ggtagctggt
    11581 cgcccgtcct ccgacctgaa agacattctg attcgatttg tcatagcgat attcaagacg
    11641 cgtgatgaat gaggcaaacg gtttgtattg aactgtgccg gtgaactccc agagggtctg
    11701 ggccaccgcc ggagctgatg tgccagacgt cgcgccgaaa cagacgttgg ctcttggttg
    11761 atagtctgtc gttccctgac aggcgacgaa acctcctgca tcctcgaaaa tttcaccccg
    11821 cagacgaagg ccccactgat cggtgaaatc atgaattaaa tagacggcgg ccccatccca
    11881 gcgcgcattc ctgcttgtac tgatcgtact gctgttggat tggttggcgt aataggactc
    11941 gatgaccgtc gatgtgtggt cggtgatttg aacgctgaga taccctccag ccagaaacac
    12001 ggtacctcca ggagtggcgg agggaccgtc cctcggcccg gccaatccat agagagtcag
    12061 ccttaccttg tcggtcggcg tcacagacaa tgcatattca acaagcggat cgaaattcgt
    12121 gtccgcccgc gcagagttaa tgtaggcgat cgcaccgacc gacagggata ccagcgtgtt
    12181 gaactcatag gtagcacgga taccacgggt cgtgaatggc tgccctaacc cgaagagcca
    12241 cgaccgcgaa taatgtgcgt tacgtggact ctcgaccact tcgtagccga caacgctgtt
    12301 gaactggccg gcttgtagat taagtcctcg gccgacaggt gccagatatt gaccgtagac
    12361 ctcctggaag tccgcccagg tactgagatt cgtcccgcct atgaaatctg aatcgcggcc
    12421 gacattgaac ttcacgcgga agccgattcg atcccacccc gtcgcctctg ccttcacttc
    12481 ccgttccaat acgaactgag cgacgttcgg ccggaattgg ttggagttca catcgaagat
    12541 gcggagctga ttgatgcgat tggacggatt gttgaagttc tgggtgtacg aaccttcaag
    12601 atagccgtag aaatgcagcc ggaagctttc cgacaatagc cgcttctcga ccgccttttc
    12661 gccttctttg agcctgagcg caacgtgttc cagcctttga gcaaccgggc cgggcgtgtc
    12721 ttcggcccat gcgtcggcaa gaagcgaccc gagggcaagc atgcaagtaa cgaatagcgt
    12781 ggagtaacac aatgcggggc agagagtcga gcggccgtac gacacagttg cttcttaccc
    12841 tattactgct ggaacgggca agaaatggag cacggctcgg aacatgggtg aacacccaag
    12901 ccccacgccc aacaaccaag cacgcctatc tcttattctg ctttgtgaat ttcgtccctt
    12961 ccagggaaag gttatacatc agccccttct ggcctaagac aaaggcgaca atcgggtctt
    13021 tcagattgtt cgtatcgata gtaccgcctg ctcccaaagt gacgagggaa accgagccgt
    13081 cgacccccgc cttccagcct tcgctgtccc ggaatcgttt cagagctccc tcatccaaaa
    13141 acacgaggta gacagtctta atttcccctc caagttgaaa tccaaatgac ccctgcagca
    13201 tgctgtagta atccgccgtc ttgccgccaa tccggagtgc cccctctcca tactcccctc
    13261 cgaaaccggc tcctcccttg agcactgttg ggaacaccag catccctttg ctgctctcca
    13321 tgaaactttt gccacctttg acttcttgtt cgaacttcgc gatcgcgtca tccacacccg
    13381 catcaatttg cctggcggat cctgccgatg cagggccgga caaccatcca gtcaacatca
    13441 aggtcatcgc cgcaagtgtg gcgatgaacc gccgcttacg agaggcgtgt cttttcatga
    13501 tcatccgctc cttccgccaa gggtcggtgg caatccatcg actcgatgat ttgttctagt
    13561 gaagtgctgt atagcgagag actctacttg taatgccgat gcttggatac tgtcaaaact
    13621 aacaggaggt gagtgcttac cagtcatcag cgttgattgc gggcacatga tccatgcttc
    13681 tgacagcact gcctatcgga gtgcgtcatt gagcaggttt gccaggcgag cccccgcgag
    13741 gccgatgcgc tgggccgcaa ctttctgtgc cgcctccttg tagccgtcgg tcagctcaaa
    13801 gggaccaacg cctatctcga cgggtgagac gtagacgtgc gttcgtgcgg cctcgaagct
    13861 ttcctggatc cagatacgtt cgtcgctgat ggcggcaagc tccggatcgg cggcgggcaa
    13921 ttgcttagct gtctcgatgg cggtcgtcgt ctccgtgctc accccgagga cattatccca
    13981 gaaggcgtgc agctttaatc gtatttcgca cgggtgacta cacaccataa ccccgtttcc
    14041 tccgtcgtcc ccgttcatgt gtgctttatc aaagcgagaa accgcgtgta agggctgatg
    14101 aacatcggcc acgagatgta tgagccatac gaggtcgtaa gatttgagag agtcggaact
    14161 cgcagtggat ctcagcgctt ggcggaacaa cgcaatttgc gtttgtgcgt taggagtcgg
    14221 cggatcggtg aacatgacat tgtccggcga gaagggcagg tcaacaaagt gccagtactt
    14281 gtgcatgtat gtatcggtat agccgatgtt ccgggcagcg ttgagccctg cgggacggtt
    14341 cccgccttcg ctcccatcct gctcgtacgc gggatcccgt ttgatgtcat cggcccatcg
    14401 agacgctcga agaaaagcga ttcggggctt ttcatcttcg ggaacattgg ctacccacgt
    14461 cgcatgcttt ggattgagct tgagtaatgt tgtgactttc tgccgtgtct cgggtgtcaa
    14521 ctgctcatag gcaattacgc ccgccatcat atgcccgaga tcgccccacg ccgccgcatg
    14581 cgcgacgaca cctacgagag ccaggatgac gagtaaaagc gttttcatct accgtgctga
    14641 acctcccacc ttgggcgcga gcacggacga gctgattact gacaggacct tccgtctaat
    14701 catactgtga gatgctgccg caccatttcg gcgctcaact catcgctctt gcccgccgcc
    14761 accaccgcgc ctttggccat gacgacatat ttctcagcca gtctggcggc gaaatggagg
    14821 ccttgttcaa ccaataagat agcgaaccga cgggacttct tgaagccgag aatgacgtct
    14881 tcgatctgat cgacaataga aggctggatg ccttcggttg gctcgtccag caagagaagt
    14941 ttcggattgg atagcaaggc tcggccgatc gccaattgct gctgctcccc accgctcagc
    15001 actccaccgg ggcgatgcag gatctgggtg agtttcggaa acaactggta gacttcgtcg
    15061 aaggccgcct tttctgtcgc cccgttctgc cgatcggatc ttgcccaaaa tcccagcagt
    15121 aaattttcgc gtaccgtcag atgcggaatg atttctcgtc cttgtgggac gatcgcgagc
    15181 ccgcggcgcg ctcggcggtc ggtggagagc tttgtgatat tactcccatt gaacaccatg
    15241 tctcctgaac gggccgacag cagtccggta atgactttga gcgtcgtcgt ttttcccacg
    15301 ccgttccggc ccatcaagca gacaacctcg ccgtccgaaa cggtgaaagt gacatcacgg
    15361 agaatgtgac tttccccgta ataggcgttg atctgcttca gttccagcat ggccccttca
    15421 ttccgtggct tgcgggatta tcaaggagca ttttccatca cgagaacgca gctggactct
    15481 tgtcattgcg ctcacccgtg cgcgaccttt tgcctgccca aatagatctc tctcactcgg
    15541 tcgtcggact gaaccttttc caccgtcccc tcacaaatga ccgtaccttc gtggaggacg
    15601 gtgacgatgc gggcgatttg ccggacgaat tccatgtcgt gttcgatcac gacgatcgca
    15661 tgtttttcgg ccaatgattg caggagccgt ccggtttgtt ccgtttcttt gtccgtcatg
    15721 cctgctaccg gttcgtccac caataagaga gaggggtcct gcaagatgac catgccgatt
    15781 tcgagccatt gcttttgacc gtgggacaag gatcccgccc tcatgccggc gtagtcgcct
    15841 aagccgatcg tcatcaatgt ctcggcgatc ttctcccgtt cctctgccgt tgacgtgccg
    15901 agcaaggtag agaatacccc cttgctgggc cgtttgagcg agagatcgag attttcccag
    15961 acggtcaaat tctcataaat cgacggcgcc tgaaattttc tgccgatacc aagcttgaca
    16021 atctcatctt cgcgcttccc gatcagctcc tgatccttgc cgaaaatgac ccggcccgcg
    16081 actggcctgg tttttccgca aatcacgtcg agcaacgtcg tttttcccgc tccgttcggt
    16141 ccgatcacga cacgcagttc attgtagtcg acgatgaagt tgaggttgtt gagcgccttg
    16201 aagccgtcgt agtcgacgac gacgccttcc aaataaataa tggagccccg gtcgctcatc
    16261 gcagtccttc ttcggcagac agggcggtcg ctctcatgtt ttggaatcgt tccttcagat
    16321 ggcgcatgat gccaaccagc ccgtcgggaa acaggaggac gaccacgaca aagagcccac
    16381 cgaggataaa cggccacagc tccggaaaat aattggtcag aaggctgcgg ccaagattga
    16441 cgctgaccgc ccccagcacc gcccccgcca atgttccgcg tccgcccacc gcgacccaaa
    16501 tgaccatgtc caatgagggc aggacaccga tctgagccgg cgtgataatc ccgacttgcg
    16561 ggacatacag cattccggcg aggccagcca aagccgcggc gacgacaaac acaaataact
    16621 tatagtttgc cggcgtgtag cctgagaaca cgactcgcgg ctcgctatct cgcacggcga
    16681 tcaacactcg tcctgcccgc gaacgggtaa tccacaagca cagcagatag gcgccaccga
    16741 ggcagagcac cgtcaggaca tacaaggccc gctgcgtgcc cgggtcggaa agtcggaagc
    16801 cgagcagttg cttgaaatcg gtcaagccgt tcgtcccgcc gacgttcgtc tcattacgat
    16861 tgaacacgag ccacgccaca agggccagcg cttgggtgat aattgcaaag tacacgcccc
    16921 tgatgcggct cctgaaggcc agaaatccaa agaccagcgc aaacgcggtc ggcaccagga
    16981 ttgctcccaa aatcgccacg ggaaaactgt agaacggttt ccaaaaaagc ggcaatgctt
    17041 tgacctggtt ccacaccata aaatccggca gctcactgcc gtagacactt tccttaccga
    17101 tcgtcagcat gaggtgcata cccatacagt aggcacccaa cccgaaaaac acgccctgtc
    17161 cgagactcag gacaccggta tagccccata tcaagtcaag cccgagcgcg aggatggcaa
    17221 aggcgaggaa tttcccgaac cgattgaggg aaaaatccga gaggtgaaac caggagtctt
    17281 cggcgggcat cacattcaat accggcatga gaatgagcaa gacgaagccg gcgacccaga
    17341 aggcaaggcc gacatctgcg cgtgacgctg aagggaggtg gtcgtggcta ggcatgtcca
    17401 acgagcgaat catccgctat acatcggcat gacgcccttt aatggcgaaa agtcccgatg
    17461 gccgccactg caagaagaga atgaccagca tgagaatgca caccttgcca tacacagcgc
    17521 cgaggctcgg ctcgagcagt ttgttcaggc cgccgatccc caacgaggcg acaatggtgc
    17581 ccgccagttt tccgacgccg ccggttacga cgaccataaa ggaatcgacg atatagtttt
    17641 gtcccagtcc tggctcgaca tttccaataa gcgtcaacgc ccacccggca acgcccgcca
    17701 gacccgaccc aaacgcaaaa gtataggcat cgacctttct ggtcggaatg cccaggcagg
    17761 cactcatgtt tcggttttgc atcacggccc ggacgcgcaa tccgatgccg gaccgaaaca
    17821 acatggcata gatcgccagg acacagagga acgaaagcgc gataatgaac agccggttgt
    17881 ttggcagata caccccaacc atcacttgca caccgccgtt cagccaactc ggtgccacga
    17941 cggctgtcag gtcgccgaaa tacacacggg cggcttgcat gagcacgaga ctgatgcccc
    18001 acgtcgccag catcgtttct aacggcctgc cgtacaaaaa gcggatcacg gtcacttcga
    18061 gtaacatacc gcaagctgcg gcaaccagaa atgccatcgg cattgcagcc gcgaaataat
    18121 aatcgaacat cgcggtcgga agaaatgcct tgaagagctc ctgtgtgatg aatgttgcgt
    18181 acgcgccgac catcattaac tcgccatggg ccatgttgat gacacccatg aggccgaaca
    18241 cgatggccag cccgacggac atgatcaaga ggatagaact gaggctgacg ccacggaaga
    18301 tcgtttcaat ggcgcttgac caggcagccc aggtctcgat gcgctcaatt gaagcgctgg
    18361 ccaccttcgc caaagcttgc tgcgcttccg ttgcttccgc cgcgctaccc aaggtgacga
    18421 actgctgcaa tgccggcacg gcgttctggc ttgccagatc tccaagagtg cgcacagcgg
    18481 agactttggc ggcatggtca tcccctgcca gatggagcag agcggcggac tcttgcatcg
    18541 tgtaccgtac ccacttgttt gactcggtcg ccgccgcatg atcgagccac ggaatcgcca
    18601 ctgtcttccc cgaggtcccg agatccgtcg ccgcggaccg acgatgatcc ggatctggac
    18661 tcgacagttt ggctcgattt ctgagcaggt ccatcacggg tttgatggcc agccgcacag
    18721 ggcggtcggc attcgcacgt atatcatcaa gccgcggaat caacgtgcta tcgcccagg
//
LOCUS       DASTQY010000009         4846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_599900, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000009 DASTQY010000000
VERSION     DASTQY010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599900"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="VFR82_00280"
     CDS             complement(<1..1063)
                     /locus_tag="VFR82_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU4500061.1"
                     /translation="MSHFRVANERRNDIRNIAIIAHVDHGKTTLVDALLRQTHVHRKI
                     DDMGERIMDSMDQERERGITIRAKNASVIHKGVKINIVDTPGHADFGGEVERTLRMVD
                     GVLILIDAKEGPMPQTTFVLRKALALGHKAIVVINKIDRPDAVIDDVVNRTFDLFVHL
                     GATDEQLDFPLVYTSAIKGIATLDINKPGTDISPLLDTVLEKIPGPAINANGPLQILV
                     LALLYDSYKGKMGVGKIQSGSIARRQQVLMLGKDGAAAPGKVSDLAVYSGLERTDVEE
                     AKAGEIVAVAGLDQVSIGDTIADAVQPSALPRVTIDEPTVQMTFSVNNSPFAGREGKF
                     LTSRHLRERLFKELETNVSL"
     gene            1220..3604
                     /locus_tag="VFR82_00285"
     CDS             1220..3604
                     /locus_tag="VFR82_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU4500062.1"
                     /translation="MTLIDRPVQLSDTTRQPPPRRPWRRWQITLITLAALFLGALLAT
                     VGLLWYYAQDLPELSQLQNYQPSLVTQVYSSDKQLIGQFFIERRILTPLAQIPEHLRR
                     AVIAVEDVRFFEHPGLDYIGILRAAWTNLRRGGKVEGASTITQQLARSLFLSSERTFD
                     RKVRELVLAYKMELVSTKEQILETYLNQIYFGQGAYGVASAAQSYFGKDLAGLTVAES
                     AFLAGLPKSPNHFSPFKNYARAKKRQEHVLARMEEAGFLTSAQREEALAEPLSFRRPG
                     SEQTAPYFVEYVRQLLVAKYGEAMVYKGGLRIKTTLNLGMQRAAEAAFKTGLRELDKR
                     QGWRGPLRTVDPATLTMGNQPVATIDQPVVAGDEREAVVTKSGKDLFWVQIGNVSAKL
                     LYDDMAWAKRRLTGPDMAKDVAVAPTPKQVLKPGDVIEVKTKKIDKEQVYVQLEQTPL
                     VEGGLIALDPDKGAIRAMVGGYDFNRSEYNRAIQAHRQPGSAFKPIIYATAINQGMSP
                     ASVVLDAPVVYEQVEEEKTWKPENYGKRFHGMVSLRDALAHSHNLATVRLLDKVGIKN
                     VIEFARQVGITSPLSADLSLGLGSSSVGLLELTSAYGVFLNQGNRAEPFAIISVTDNA
                     GEVLEQEDVQTQEVLSKETAYLITNMMEDVVQKGTGQAAKSLGRPIAGKTGTTNDFIN
                     AWFIGGTPNLVSGIFVGFDDRRSLGETESGAHAALPIWINFMKEALKTLPVAPFTIPE
                     GITFVKVDPSTGLLNSEQEDQPGNVEIFARGSEPTQAVQRRSDPMDFYKIDQPE"
     gene            complement(3614..3826)
                     /gene="iscX"
                     /locus_tag="VFR82_00290"
     CDS             complement(3614..3826)
                     /gene="iscX"
                     /locus_tag="VFR82_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEU4500063.1"
                     /translation="MDLKWQDTEDIAIRLVEEHPGTDPLTVRFTDLHAWIVALPDFKD
                     DPKKSNEKILETIQMAWHEEYQDSAS"
     gene            complement(3876..>4846)
                     /locus_tag="VFR82_00295"
     CDS             complement(3876..>4846)
                     /locus_tag="VFR82_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEU4500064.1"
                     /translation="TRDQLVTVTADIIERTLAPCRFALQDAGLSPADIDEVVLVGGST
                     RMQLVRQRVESLFGKKPHCHLNPDEVVALGAAVQADILTGGTTDMLLLDVTPLSLGIE
                     TMGGVMSSLIRRNTTIPASAKELFTTYVDGQTGVDIHILQGERELVKDNRSLARFRLK
                     VPPLPAGVPRIEVTFLIDANGILNVKASDMRTGQTQSIEVKPSYGLSDTEVERMIEDS
                     FKFAQQDVDARKLIEARLDARALIATTEKSLADGDKLIGQEEAAAIRDRLSLLRVASD
                     GNDPKAIRARMADLEQAAKPLSVAMLDDSLKRSLQGKKVSEVSKSD"
BASE COUNT         1014 a         1355 c         1386 g         1091 t
ORIGIN      
        1 ggagagagac gttcgtttca agttctttaa acaggcgctc acggaggtgt cgggacgtca
       61 agaacttgcc ttcacggcct gcgaacgggc tattgttcac cgaaaaggtc atttgaacag
      121 tcggctcgtc gatggtgacc cttggcagcg ccgatggttg cacggcatcc gcgatcgtgt
      181 caccgatgct cacctggtcc aatcccgcga ccgcaacaat ctcgccggct ttggcttctt
      241 caacatccgt gcgctcgagc ccggaataga ccgcgaggtc ggacaccttg cccggagcgg
      301 cggcgccgtc cttccccagc ataagcactt gttgccgacg tgcgatcgag ccggactgaa
      361 tctttcccac acccattttg cccttgtatg agtcgtagag gagtgcgagc accaatatct
      421 gcagcggccc gttcgcgttg atcgccggac caggaatctt ctccagcacc gtatcgagca
      481 ggggcgagat gtccgtgccg ggcttgttga tgtcgagcgt agcgatccct ttgattgccg
      541 acgtgtagac gagagggaag tccagttgct cgtcggtggc cccgagatgc acgaacagat
      601 cgaaggtgcg gttcaccacg tcgtcgatga ccgcatcagg tcggtcgatc ttgttgatga
      661 ccacgatggc cttgtgcccc agtgccagcg ctttcctgag cacgaaggtg gtctgcggca
      721 tcggtccttc tttggcgtcg atcagaatga ggacgccgtc caccatgcgc agtgtgcgct
      781 ctacttctcc gccgaaatca gcatgaccag gtgtgtcgac aatgttgatc ttgacgcctt
      841 tatggatgac gctggcattt ttagcacgga tcgttatgcc ccgttcccgc tcctggtcca
      901 tggaatccat gatgcgttcg cccatgtcgt cgatctttct atggacgtga gtctgccgta
      961 acagggcatc aacgagcgta gtctttccat ggtccacgtg ggcgatgatc gcgatgttgc
     1021 ggatgtcgtt ccggcgttca tttgctacgc gaaagtggga catatcctgg tatcctaagg
     1081 cgaaaaaaaa gacgccccga ctatcgggcg tcgacagagc agtataccgg aagcactgag
     1141 cgcctacaag gtgccggttg cttgtgtttt ggcagactcc acggtatgtt gagccgagag
     1201 tccttccgaa atcagttcca tgacattgat agatcggcca gttcagcttt ccgatacgac
     1261 cagacagccg ccccctcgac gtccttggcg ccggtggcaa atcacgctga tcacgctggc
     1321 ggcgctcttc ctcggcgcgc tgctggcgac tgtcggctta ttgtggtact acgcccagga
     1381 tctgccggag ctcagtcagc ttcaaaatta ccaacccagt cttgtcacac aagtgtattc
     1441 cagcgacaag caattgatcg ggcaattttt catcgaacgg cgcattttaa cgccgctggc
     1501 gcaaattcca gagcatctac gacgtgccgt gatcgctgtt gaagatgtgc gattctttga
     1561 gcacccgggt ttggattaca tcggcatcct tcgtgctgcc tggaccaatc tgcgccgcgg
     1621 cggaaaggtg gagggtgcca gcacaataac acagcagttg gcgcggtcgc tgtttttgtc
     1681 ctccgagcga accttcgatc gaaaagtccg ggagttggtg ttggcctata agatggagct
     1741 cgtctcaacg aaggagcaga ttctcgagac gtatctcaac caaatctact tcggacaggg
     1801 ggcgtatggg gtcgcgtcag cggcccagtc ctactttggc aaggacctgg ctggactcac
     1861 cgtcgccgaa tccgcctttc tggcgggatt gccgaaatct ccgaatcatt tctctccctt
     1921 caagaattat gcgcgtgcca aaaagcgaca agagcatgtg ttggcgcgga tggaagaagc
     1981 gggttttctc acatcggccc agcgtgaaga agctttagct gaaccattga gtttccggcg
     2041 ccccggtagt gaacagaccg ccccctactt cgtggaatac gtgcgacaac ttctcgtcgc
     2101 aaaatacgga gaggctatgg tctataaggg cggactccgg atcaagacga cgctcaatct
     2161 cggcatgcag cgtgctgctg aagcggcctt caaaaccggc ttacgggagc tcgacaagcg
     2221 ccagggttgg cgcggccccc tgcgaaccgt ggaccctgcc accctgacga tgggcaacca
     2281 gcctgtggca acgatcgatc agccggttgt cgctggtgat gagcgggaag cagtggtcac
     2341 caaatccggc aaagacctgt tctgggtgca aatcggcaat gtctccgcca agcttctcta
     2401 tgatgacatg gcctgggcca aaagacgcct cacaggaccg gatatggcga aggatgtcgc
     2461 cgtggcaccc acccccaagc aggtgctgaa gccgggcgat gtcatcgaag tcaagactaa
     2521 aaagatcgac aaagagcaag tctatgttca actggagcag actccgctgg ttgaaggagg
     2581 actgatcgca ctcgacccgg ataaaggcgc aatccgcgcg atggttggag gctacgactt
     2641 taaccgcagc gaatacaacc gcgctattca ggcccatcgg caaccagggt cggccttcaa
     2701 gccgattatc tatgcgacgg ccatcaatca ggggatgagc cctgcctctg tcgtgctcga
     2761 cgcgcccgtt gtctatgagc aggtcgagga ggagaagact tggaagcccg agaactacgg
     2821 gaagcgcttc cacggtatgg tgagtctacg ggacgcgttg gcccactctc ataatctggc
     2881 cacggtcagg ttgcttgata aggtcggaat taagaacgtt attgagtttg cgcggcaggt
     2941 cgggatcacc agtccgctgt ctgccgatct ctcgttggga ctggggtctt cttcggtcgg
     3001 cttgcttgaa ctgacgtcgg cttacggagt cttcttgaac caaggcaacc gggcagaacc
     3061 ttttgccatc atctcggtga cggacaacgc gggcgaggtt ctggagcagg aggacgtgca
     3121 gacacaagag gtcttgtcta aagagacggc ctatctcatc acgaacatga tggaggacgt
     3181 cgtgcaaaag gggaccggtc aggcggccaa atcgctcggt aggccgattg ccggcaagac
     3241 aggcactacg aacgatttta tcaatgcgtg gttcatcggg ggaactccga atctcgtttc
     3301 gggaatcttc gtgggctttg acgatcggcg ttcgctcggc gaaaccgaat caggggctca
     3361 tgcagccctg cctatatgga ttaacttcat gaaggaagcg ctgaagactc tgccggtcgc
     3421 accgtttacg attcccgagg gtattacatt cgtcaaggtc gatccctcca cagggctgct
     3481 aaattcggaa caggaggatc agccgggaaa cgtcgaaatc tttgcgaggg gcagtgaacc
     3541 gacccaggcg gtgcagcgcc gctcggatcc gatggacttc tataaaatcg atcagccaga
     3601 atagcgcagg gagtcaggac gcagagtctt gatattcctc atgccaggcc atttgaatcg
     3661 tttccagaat cttttcgttg gattttttcg ggtcgtcctt gaaatcgggc aaggcgacga
     3721 tccacgcatg caggtcggta aatcgtacgg tcaacggatc tgttcccggg tgttcttcga
     3781 cgagcctgat cgcgatatct tctgtgtcct gccatttcag gtccatgtac aactcctcgc
     3841 ctttagccct gtcaatctct tcctgggcct gaatatcaat cacttttcga gacttcggag
     3901 acttttttcc cttgcaagct ccgtttgagt gaatcgtcga gcattgcgac cgacagcggt
     3961 ttggccgcct gttctaggtc ggccatccgt gcccgaatcg ctttcgggtc gttgccatcg
     4021 ctggcgactc tgaggagaga tagcctgtcc cgaatggctg cggcttcctc ctgcccgatg
     4081 agcttgtcac catcggccag cgacttttcc gtagtagcga tgagggcgcg ggcatcgagc
     4141 cgcgcttcaa tcagtttccg ggcgtcgacg tcttgctgcg cgaacttgaa cgaatcttcg
     4201 atcattcgct cgacttctgt gtccgataag ccataggacg gtttcacttc gatcgattga
     4261 gtctggcccg tccgcatgtc gcttgctttc acgttcaaaa taccgtttgc atcaatcaaa
     4321 aaggtcacct cgattcgcgg aacgcccgcc ggaagcggag ggactttgag gcgaaatcgt
     4381 gcgaggctgc ggttatcctt gacgagctcg cgttctccct gaagaatatg aatatcgacg
     4441 ccggtctggc cgtcaacata cgtagtgaac agttctttcg cgctggccgg aatggtcgta
     4501 ttccgtctaa tgagactgct catcacccca cccatcgtct cgatgccgag cgacaatggc
     4561 gtcacatcaa gcagcaacat gtcggtcgtg ccgccggtga gaatgtcggc ctggacggca
     4621 gcacctaggg cgacaacttc atcggggttc aagtgacagt gaggcttttt cccgaagagc
     4681 gactctacgc gctgacgcac gagctgcata cgagtcgatc ctcccaccaa caccacttcg
     4741 tcgatgtcgg caggcgacag ccccgcgtct tgtaaggcaa atcggcaggg cgccagcgtc
     4801 cgttcaatga tgtccgccgt caccgttacc agttggtccc tcgtta
//
LOCUS       DASTQY010000010         7003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1606843, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000010 DASTQY010000000
VERSION     DASTQY010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7003
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606843"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFR82_00300"
     CDS             complement(<1..232)
                     /locus_tag="VFR82_00300"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248583.1"
                     /note="TrpG; with TrpE catalyzes the formation of
                     anthranilate and glutamate from chorismate and glutamine;
                     TrpG provides the glutamine amidotransferase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate/aminodeoxychorismate synthase
                     component II"
                     /protein_id="HEU4500065.1"
                     /translation="MLLMIDNYDSFTYNLVQYLGELGEDVRVYRNDKISIEQIESLKP
                     TRIVVSPGPCTPNEAGISVETMKHFAGRLPLLG"
     gene            complement(315..1811)
                     /gene="trpE"
                     /locus_tag="VFR82_00305"
     CDS             complement(315..1811)
                     /gene="trpE"
                     /locus_tag="VFR82_00305"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU4500066.1"
                     /translation="MSVPRYSLSLEQFRHYAGQGNLIPLYREILADFETPVSAFAKID
                     HGPSAYLLESIEGGEKWARYSFLGSGSPVVIYEDRGDLVQVGGKRRRRILSRGNPLER
                     LREVMAAYRPVTVPDLPRFVGGAVGYLSYDMVRTFEELPGRQKEHTNLPDFAFLLTDT
                     LLIFDNVSHKIKVVANAHVASTRERDVREAYRAATDRIERMIARLKRPLARTRPHRRR
                     LPITFTSNMNRADFEKMVTRTKEYIRSGDVVQAVLSQRWETNVQAPPLQLYRALRVIN
                     PSPYMYYLRVAGLELVGSSPETLVRCEDGVISVRPIAGTRRRGQSTEEDQQFERRLLA
                     DEKERAEHVMLVDLGRNDVGRVARPGSVTVDSLMHVERYSHVMHIVSNVTGQLDSGKT
                     SYDVLRACFPAGTVSGAPKIRAMEIIDELEPTRRGPYAGAVGYFSFSGNMDMCINIRT
                     VVIKKHQAFVQAGAGIVADSNPEHEYEETCNKARAMMKAIELAEQGLE"
     gene            complement(1823..2461)
                     /locus_tag="VFR82_00310"
     CDS             complement(1823..2461)
                     /locus_tag="VFR82_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU4500067.1"
                     /translation="MMEAMSVRPSLRQAARVVALICGGLFLSGCVLSEKYEAEKARSL
                     NFQRLLAQEEKRTGELDNEVKRTKRELAEYETRNRELTAQVQAVREQMSRVQEESEAI
                     KEASLLERKAKADMKRSAPPAVKAPPVEPTPNISELLGTASNAQSDTLPSTTDAQVEA
                     KGASTIHVVKPGETLYRISRRYGVKVDTLRKWNKLPDDIIEVGQKLVVSQEQ"
     gene            2700..4703
                     /gene="ligA"
                     /locus_tag="VFR82_00315"
     CDS             2700..4703
                     /gene="ligA"
                     /locus_tag="VFR82_00315"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU4500068.1"
                     /translation="MVTTDIQARVAALREEIRRHDYLYYVQAKPQISDSAYDRLFREL
                     TELEQAHPELVTADSPTQRVGAPPLDSLTKVRHEQAMLSLDSMVEQSDVLAFDQRMKR
                     ELGVPTVGYTAEPKFDGLSVELLYERGFFTRGATRGDGVTGEDVTVNLKTIRALPLQL
                     MPGTGYPDRLVVRGEVYMKLDDFQAMNRKLTERGEDGFANPRNAASGALRQLDSAITA
                     SRPLALTCYEIMAWSGKGLPTHSDELEALARWGFPIPLHRLRCDSIQEVIAYHRDTEH
                     MRDSLPYEIDGVVIKVDRRDWQEQLGYKSRSPRYAIAYKFTPRKEITRVQDIVVSVGR
                     TGTLTPLALLTPVEVGGVTISRATLHNADEVARKDIRVGDTVRVERAGDVIPAIAERV
                     PIPDERRGEPFRMPDHCPVCQSSVAREGAYYYCTGQSVCMAQLKGAIEHFASKSALNI
                     AGLGRKTVAQLVDAGLVRSLADLYRLTKEQLLGLEGFGERSAGLLLEAIERSKRVSLD
                     RFLMGLGIRQVGQHIAKVLAREFGSLTRIIDSDDARFQQVAAIGPEISSSLVSYFSEA
                     HNRRVIQDLLDSGFDIPAETDASSARQTLAGKTFVFTGGLKDLTREQAGKLVEERGGR
                     IASSVSKKTAYVVAGADAGSKLEQAAKMGITVLTEEEFRQLVE"
     gene            complement(4716..5999)
                     /locus_tag="VFR82_00320"
     CDS             complement(4716..5999)
                     /locus_tag="VFR82_00320"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU4500069.1"
                     /translation="MDIAILLGLIVLSAVISTAEIGFFAVNETRLRALAQNQNSRAKM
                     ALYLRSDPQKLLATILVGDRLVGTAIPMYATFLTLNTYGADRTFFDDTIAVVVGLLTF
                     VLLVSVDVVPKTLAAKFAVPVTLNMAYPVYGVQLVLKPILALMVPFIHRLTGGKGLTL
                     PLVTEEELKIMLDEGGKTGAIEVEKVKMIKNVFQLKDITAEDAMTPRLYVFSLDGNLR
                     LKEAQELLYNSKYSRIPVYDGTLDNITGILYKSRALTELAKNQTDAKLKEIAYPPIFV
                     PAGKTADDLMKQFQQEKRHMAVVVNEFGGVMGIVTLEDLLEEVVGEIMDETDITEELI
                     KRLGKNHILVHGRTEVRKINDFLKVELGDEAVTISGLIQQELGRIPKVGEELRIGNCR
                     IVIHEAEPRSIRSVQIYREEKTPVVVEAPNLNLVS"
     gene            complement(6013..6147)
                     /locus_tag="VFR82_00325"
     CDS             complement(6013..6147)
                     /locus_tag="VFR82_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500070.1"
                     /translation="MKLLVPPKVFRIDLGISAMCLACPRAGSGFLAPICQTTVQVTRP
                     "
     gene            complement(6232..>7003)
                     /locus_tag="VFR82_00330"
     CDS             complement(6232..>7003)
                     /locus_tag="VFR82_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEU4500071.1"
                     /translation="SSSHVRFIVSGTWLMPICPPGSSDGRVCLLTISRSLLSGFRFSP
                     AFVSALVVTATVIGCSSPPKPTYFPGYPIGYVEGGVASWYGPGFHGNRTANGERYDMH
                     KLTAAHRTLPLGSIATIRSATTGREVTVRINDRGPFAKGRVLDLSMAGAQALGMIGSG
                     TDRIELRVIGFQGRTADMGFLRVQIGSFVEQQNALNLLERARRLYPGGRIQPVDLPEG
                     RRYRVHVGEFKTEHQAESAARRLEADLATQSFVFRDDN"
BASE COUNT         1516 a         1952 c         1982 g         1553 t
ORIGIN      
        1 cgcccagaag cggcaagcgg ccggcgaaat gcttcatggt ctcgaccgag atacccgcct
       61 cgttcggcgt gcacggtcca ggcgatacga cgatgcgagt aggcttgagg gactcgattt
      121 gttcgatcga aatcttgtcg ttgcgataga ctcgcacgtc ttcccccaat tctcccaaat
      181 actgaacgag attgtaggtg aacgagtcgt aattatcgat catcagcagc atggtgatca
      241 gtggtctctg tgggcagaag ggccgagtac cgtcgcctcg tgattgacgt cgctgcctgg
      301 tcggctctcg cggctcattc caatccctgc tcggccagtt ctatcgcctt catcatggcg
      361 cgggccttat tgcatgtctc ttcgtattca tgctcaggat tggagtcggc cacaattccg
      421 gccccggctt gcacgaaggc ctgatgcttc ttgatgacga cggttcggat gttaatgcac
      481 atgtccatgt tgcctgaaaa actgaaatac ccgactgccc cggcgtaggg ccctcgtctg
      541 gttggctcca gttcatcgat aatctccatg gcacggatct ttggtgcacc ggaaaccgtc
      601 cccgccggaa agcaggcgcg caagacatcg taggacgtct tcccagagtc cagctgtccc
      661 gtgacgttag agacgatgtg catgacgtga gaataccgtt ctacgtgcat gagcgagtcg
      721 acggtcacgg aaccgggacg ggcgactcgt ccgacgtcgt tgcgccccaa gtcgaccaac
      781 atgacgtgct cggcccgttc cttttcgtcc gccaacagac ggcgctcaaa ttgctggtcc
      841 tcttctgtgc tttggcctcg gcggcgtgtg ccggcgatgg gtcgtacgga gatgacgcca
      901 tcttcacagc gcacgagtgt ttcgggagac gaaccgacca gttcaagccc cgccacacgc
      961 agatagtaca tatagggaga cggattgatc acacgaagag cccggtacag ttgcagcgga
     1021 ggggcctgaa cattggtttc ccatcgttgg gacaagacgg cctgcacgac gtcaccggat
     1081 cggatgtatt ccttcgtccg tgtcaccatc ttctcgaagt ccgctctgtt catgttcgac
     1141 gtaaaggtga tggggagacg ccgtcgatga gggcgtgtcc tggcgagcgg acgcttgaga
     1201 cgtgcgatca tccgttcgat gcgatcagtc gccgcacgat aggcctctct gacatcccgt
     1261 tccctcgtgg acgcgacatg cgcgttggcc acgactttga tcttgtgaga gacattgtcg
     1321 aaaatcagca aggtgtcggt caggagaaag gcaaaatccg gcagatttgt gtgctccttt
     1381 tgcctgccgg gcaattcttc aaacgttctg accatgtcat agctcaaata tccgacggcg
     1441 cctcccacaa aacggggaag gtccggcacg gtgacgggac ggtatgcagc catgacttcc
     1501 cgcagtcgct cgagcggatt tccccgactg aggatgcgtc ttctccgttt gccccccact
     1561 tgtacgagat ccccgcggtc ttcgtaaatg acgacgggcg agccgcttcc caaaaaggaa
     1621 tagcgcgccc atttttcgcc gccttcgata ctttccagca gataagcact gggcccgtga
     1681 tcgatttttg cgaacgcgga gacgggagtc tcaaaatcgg ccaggatttc gcgatacagc
     1741 gggatgaggt tcccctgtcc cgcgtagtgg cggaattgct cgaggctgag tgaataacgt
     1801 ggtacagaca cggccgctgt gcctattgtt cctggctgac gactaatttc tgtccgactt
     1861 cgatgatatc gtccggcagt ttgttccact tacgcagcgt gtccaccttc acgccgtaac
     1921 gccggctgat gcggtacagc gtctcgccgg gcttgacgac atggatcgtg gaagcgcctt
     1981 tcgcttcgac ttgcgcgtcg gtcgtcgaag gtagcgtgtc cgactgtgca ttggacgccg
     2041 ttcccaataa ctcgctgatg tttggtgtgg gttcgactgg gggagctttg acggccggag
     2101 gtgcggatcg tttcatatcc gccttcgctt tgcgttcgag aagcgaggct tccttgatcg
     2161 cctcggactc ctcctgaaca cgcgacatct gttcgcgcac agcttgaact tgagcggtta
     2221 actcccggtt acgcgtctcg tattccgcca actcgcgctt ggtccgcttc acttcattat
     2281 caagctcgcc ggttcgcttt tcttcctggg caagcagccg ttggaagttc agacttcgcg
     2341 ctttttctgc ttcgtacttt tcagacagca cacagccgct caaaaacagc cctccacaga
     2401 ttagagcgac gacccgtgcc gcctgtcgaa gcgatggtct gacgctcatt gcctccatca
     2461 tggctacctc ctcatcgcac gtggactctc tcgtgctatc cgatcgaacg cggtccttgc
     2521 ggttctgtag tataaaacct tcagtaggat tggatagaat aatggctaac gcagcggaag
     2581 tcaaagcgaa taaggccgct tcgtcgtttg accatgcctg cgtggctatg atacggtcgt
     2641 cccacttcga ggggtccttc gcctgatcgt ctgcgccggt tcctccaaac tcagtctcca
     2701 tggtcactac cgatatccag gcccgagtgg ctgccttgcg ggaggagatt cgacggcacg
     2761 attatctgta ctatgttcag gctaagccgc aaatctccga ttctgcctac gatcgcttgt
     2821 ttcgagaatt aacagaactc gagcaagccc atcctgagct ggtgacggcc gactccccga
     2881 cccagcgtgt gggggctcct cccctcgatt ccctgaccaa ggtccgtcac gaacaggcga
     2941 tgttaagcct cgattccatg gtcgaacaga gcgatgtctt ggcatttgac caacggatga
     3001 agcgggaatt gggtgtgccc acagtcgggt ataccgcaga gcctaagttt gacgggctct
     3061 ccgtggaatt gctctatgaa cggggcttct ttaccagagg agcgaccaga ggcgacgggg
     3121 taacgggaga agatgtcacg gtgaacttga aaacgatccg ggccttaccg ctgcagctga
     3181 tgccgggaac gggctatccc gatcgtcttg tggtgcgtgg cgaagtctat atgaagcttg
     3241 atgactttca agccatgaat cgaaaactga cggagcgcgg cgaagacgga tttgcgaatc
     3301 caagaaacgc ggcctcaggc gcgttgcgcc agctggattc tgctatcacg gcctcccggc
     3361 cacttgccct gacttgctat gaaatcatgg catggtccgg caagggattg ccgacacact
     3421 cggatgagct tgaggctctg gcgcgatggg ggttcccaat cccattgcac cgcctcagat
     3481 gcgactcgat tcaagaagtg atcgcctatc accgagacac tgaacacatg cgcgacagtt
     3541 tgccctacga aatcgacggc gtggtcatca aagtcgaccg gcgcgattgg caggaacaac
     3601 tgggctacaa atcgagaagc ccgcgctacg caatcgctta caaattcact cctcgcaagg
     3661 aaatcacgag agtgcaggat atcgtcgtgt ccgttggacg gaccggcacc ttaacgccgc
     3721 ttgccttgct gacgccggtc gaggtggggg gcgtaacgat cagccgagcg actcttcaca
     3781 acgcggatga agtggccaga aaggacatac gtgtcggcga tacggttcga gtcgagcggg
     3841 ccggcgacgt cattccagcc atcgccgaac gcgtaccgat ccccgatgag cgccggggag
     3901 aaccgtttcg gatgccggat cactgtccgg tctgccaatc cagcgtcgcg agggaaggtg
     3961 cctattatta ttgcaccgga caatcggtct gtatggccca actcaaagga gccatcgaac
     4021 actttgcgtc gaaaagcgct ttgaacattg cagggctcgg aaggaaaacg gtggcacaat
     4081 tggtcgatgc aggactggtt cgctcgcttg cggatctcta caggctgacc aaagaacaac
     4141 tgctcggtct ggagggtttt ggcgagcggt cggccggcct gttgctggag gccatcgaac
     4201 ggagcaaaag agtctcgctc gaccgtttct taatgggtct cggcatccgt caggtcggac
     4261 agcacattgc caaagtcctc gcccgggagt tcggttcact tactcgtatc attgactcgg
     4321 atgacgcgag gtttcaacag gtggcggcaa tcgggccaga gatttcatca agcttggtct
     4381 catatttttc agaagcgcac aaccggcgcg tgattcaaga cttgctcgac agcggattcg
     4441 acatcccagc cgaaacggat gcttcatccg cacgccagac attagccgga aaaacctttg
     4501 tcttcaccgg tggcctcaag gacctgacca gggaacaagc cggaaagtta gttgaagagc
     4561 gaggagggcg aatcgcctcc agcgtgagca agaaaaccgc ttatgtcgtc gcgggagcag
     4621 acgcgggatc gaagctcgaa caggcggcca aaatgggcat taccgtcctc accgaagagg
     4681 aatttcggca gctcgtcgaa tgaccatagc ccgcttcagc taaccagatt taaattggga
     4741 gcttctacga caaccggggt tttctcttcg cggtagattt gcacgcttcg gattgagcgt
     4801 ggctccgctt catggatgac aatacggcag ttgccgattc tcagttcctc gccgacctta
     4861 gggatgcggc ccagctcctg ttgaatcaac ccgctgatcg tcaccgcttc gtcgccgagt
     4921 tcgaccttca ggaagtcgtt tattttgcgg acttcggtgc ggccatggac aaggatgtga
     4981 tttttgccca gacgcttgat caattcctcg gtgatgtccg tttcgtccat gatttcgccg
     5041 acgacttcct cgagcaaatc ttccagggtc acaatgccca tcacgccgcc aaattcgttg
     5101 accaccacgg ccatgtggcg cttttcctgc tggaattgtt tcattagatc gtccgctgtc
     5161 ttccctgccg gcacaaagat cggcggatag gcaatctcct tcagcttggc gtccgtttga
     5221 ttcttggcca gctccgtcaa agccctgctt ttgtagagaa tgccggtgat gttgtcgagc
     5281 gttccgtcgt agaccggaat gcgagaatac ttcgaattat acaagagctc ctgagcttcc
     5341 ttcagacgaa ggttgccatc cagggaaaac acatagagcc gtggcgtcat cgcatcttca
     5401 gcggtaatgt ctttcaattg aaagacgttt ttgatcatct tgaccttttc gacctcgatg
     5461 gcgcctgttt tgccaccctc gtcgagcata attttcagct cttcttccgt cacgagcggc
     5521 agggtcagcc cttttccacc tgtaagccga tggataaacg gcaccatgag cgccaagatc
     5581 ggtttgagaa ccaactgcac tccgtagacc ggataagcca tgttcaatgt cacgggcacc
     5641 gcgaacttgg ctgccaaggt tttcgggacg acgtcgaccg acacaaggag cacgaacgtc
     5701 agaagcccga caaccacggc gatcgtatca tcaaagaacg tccggtcggc cccataggta
     5761 tttagggtga gaaatgtggc atacatcgga atggcggttc ccacgagtcg atcgccaaca
     5821 aggatcgtcg ccaacagttt ctggggatcg ctacggaggt agagggccat cttggccctg
     5881 ctgttctggt tttgggccaa cgcacgcagc ctggtttcat tgaccgcaaa aaacccgatt
     5941 tcagcagttg aaattacagc agaaagaaca ataaggccca gcagaatggc aatatccata
     6001 gagcttccag gtttagggac gggtcacttg cacagtcgtc tggcaaatgg gggccagaaa
     6061 gccgctgccg gctctcgggc aggcgagaca catggcggag ataccgagat cgatgcgaaa
     6121 gactttgggg gggactaaga gtttcatgag gagtacatct atcataggca aggttagggg
     6181 gcaagcactt caacaaaagt gatcataaaa tcaaaggctt gtggatcacg cttaattgtc
     6241 gtcccgaaac acgaaggact gagtggctaa gtcagcctcc aatctcctcg ctgcggactc
     6301 ggcttgatgc tcggtcttaa actcgcctac atgaacgcga tagcggcggc cctccggaag
     6361 gtcgaccggt tggattcggc ctcctggata tagacgtcga gcccgttcca ataggttcaa
     6421 ggcattttgc tgctcaacga aggagccgat ttgcacccga aggaatccca tatcggcggt
     6481 tctcccttga aatccgatga cgcgcagttc gatccggtct gttccactgc cgatcattcc
     6541 gagagcctgg gcgccggcca ttgaaaggtc cagcacgcgg cctttggcaa aggggccgcg
     6601 atcgttgata cggacagtga cttcccggcc agtagtggcc gagcggatcg tagcaatgga
     6661 tccgagcggc agtgtcctgt gagccgccgt gagcttatgc atatcgtacc gctcaccgtt
     6721 ggcggtgcga ttcccgtgga atccaggtcc gtaccaggaa gccactcctc cttcgacata
     6781 accgatgggg tacccgggaa aataggtggg cttaggcgga ctgctacatc cgatgactgt
     6841 ggctgtgaca acaagagcgc tgacgaaggc cggtgagaac cggaagccgg agagcaaact
     6901 acgactgatg gtcagtaagc agacacgccc atcagaggat ccaggagggc agatcggcat
     6961 cagccaagta ccagagacaa tgaagcgaac atggctgcta gaa
//
LOCUS       DASTQY010000011         5191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_397502, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000011 DASTQY010000000
VERSION     DASTQY010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397502"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..798)
                     /locus_tag="VFR82_00335"
     CDS             complement(268..798)
                     /locus_tag="VFR82_00335"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HEU4500072.1"
                     /translation="MPNQRPRLRDHLRPDLHVIFVGINPGLRSAAMGHHFAGHSNRFW
                     KLLFDSGMVDEPLSFREDWRLPEWRLGLTNIIDRPSAGIDDLDPAEYRAKASTLERKI
                     RRYQPRIIALLGVTIFRILFPEITRSSRLALGPTPARLAGIPVFLLPNPSGRNAHYPY
                     RVMLDAFALLRRLAGE"
     gene            1018..1251
                     /locus_tag="VFR82_00340"
     CDS             1018..1251
                     /locus_tag="VFR82_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500073.1"
                     /translation="MTRMLFAIMAVAVAISFSAPSFAGEEKKDKKDEKKGGHVLVYGD
                     EKKDKKDEKKGGHMDTFGDEKKKDEKKGGMGGK"
     gene            complement(1325..1837)
                     /locus_tag="VFR82_00345"
     CDS             complement(1325..1837)
                     /locus_tag="VFR82_00345"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU4500074.1"
                     /translation="MTKNLYTGKVVTLNIETVTLPNGLTIDLEVVRHPGAAAVVPITE
                     DGSVVLIRQFRHAAGGFIYEIPAGKLHSGEDPRSCATRELEEEIGYKPGQLMLLSSIF
                     TAPGFTDEVIHVYKATGLTKGRQHLDRDEVLEVVEVSLEEAIGMIRAGTIRDAKTIVG
                     LQLVFTESGK"
     gene            complement(1874..2986)
                     /gene="gcvT"
                     /locus_tag="VFR82_00350"
     CDS             complement(1874..2986)
                     /gene="gcvT"
                     /locus_tag="VFR82_00350"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEU4500075.1"
                     /translation="MNSTPLIDQHRAHHAKLVDFAGWQMPIQYASVIDEYQAVRSKAG
                     LFDVSHMGRIRVSGGGSLAFLQRMTTNDVAALALCQAQYSMVCNEAGGIKDDIFVYRT
                     GETDYLLCVNASNREKILAWLSQHRATTHDQCVIEDQTSALAQIAIQGPASRDILFTA
                     GAEGISDLKLHHCREITLNDINCFVARTGYSGELGYELNVPATQAIHLWTFLLKKGEY
                     KGLKPAGLGARDLLRLEMAYLLYGNDISEETTPIEAGAEWTVNFRKGHFLGKGALDDQ
                     ARQGPPRRLVAFEVLEKSVPRHGFTILDAMSSQPIGVVTSGNLSPLLQKGIGLGYVPP
                     SLAQAGTAISIDIRGKRLPAAVVKPPFYRKIKTKNG"
     gene            complement(3007..3375)
                     /locus_tag="VFR82_00355"
     CDS             complement(3007..3375)
                     /locus_tag="VFR82_00355"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEU4500076.1"
                     /translation="MPFEQAGPEGKEVTTASGLQYIDLRMGTGATAQAGQTVSVHYSG
                     WLDNGTPFDSSLERGQPFSFPLGAGRVIKGWDEGVQGMQVGGKRKLIIPSHLGYGARG
                     AGNAIPPHATLIFEVELLAV"
     gene            complement(3407..4630)
                     /locus_tag="VFR82_00360"
     CDS             complement(3407..4630)
                     /locus_tag="VFR82_00360"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEU4500077.1"
                     /translation="MMTQTIQSNTSTALLFASALSHKTDTEAAVRDLADAVRAQLGRA
                     TLELAFVFFSPHHAKKADLVATMIQEELSIEVCVGCSGEGVIADHEEFETAPALTLWV
                     ASLPQVRVTPLRMAFSQTQDQIQMAGWPEPSAEPSTFLLLADPFTTPVHDVLSVMTDR
                     YPNGLALGGLCGGGHGPGENRLIRNAEVFDGGVVGVQLSGPIAVRPVISQGCCPIGER
                     YVVTRAEQNMIYELGGQPALSRLQEVFESLEGSQRRDAHRALHIGIAIDEHRSRFERG
                     DFLVRNLVGADQQSGALAIGDLVQEGQTIQFQIRDGKSASDDLHVLLAADRAKHPNPA
                     LGALLFSCCGRGEGLFGRAHHDSGVVQERMGPIPVAGFFAQGEIGPVGGRNFLHGYSA
                     SVAVFSRPDHAHEPQ"
     gene            complement(4646..4873)
                     /locus_tag="VFR82_00365"
     CDS             complement(4646..4873)
                     /locus_tag="VFR82_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500078.1"
                     /translation="MNEYQIGGGLRLLTAVEKTDAFGEFLKTRMVRALETEDPTELHY
                     LLAQLDDYHSYMWRYYKKLAKDRSERMNPGV"
     gene            complement(4954..>5191)
                     /locus_tag="VFR82_00370"
     CDS             complement(4954..>5191)
                     /locus_tag="VFR82_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU4500079.1"
                     /translation="IAFSTANVIPHYPQKRTYALTHLADTHLNPVITVTVEATEEAVI
                     NALTAATTVVGRDNYRVEAISLSRLRAILGTSSK"
BASE COUNT         1150 a         1422 c         1479 g         1140 t
ORIGIN      
        1 gttgaagaag agctgcgcac cctgactgga cgcggattga gcgatggccc aggcgatact
       61 gtgtttgttg gccacgccga tgatcaatcc ttttttgccg ctcagtaaca tgcgttctgc
      121 tccagcggtt gcgagaaact attccagtcg cggcgatggt aactcagggc agtgaagata
      181 gcatgattgg ccggaactgt tccaggcgag agaatggagg gcgctgagcc cgtcatgggg
      241 tgcgatcacc gggaaaagac tcccggacta ttcgccagcc agcctgcgca gtagagcaaa
      301 tgcatcgagc atcacacggt agggataatg ggcgttacgt ccgctgggat tgggcaggag
      361 aaacaccgga atcccggcaa gtcgtgccgg agtgggcccc aacgccagac ggctcgaacg
      421 cgtgatctcc ggaaacaaga tacgaaatat ggtgacgccc aatagcgcga tgattcgagg
      481 ctggtatcgc cgaatttttc gttcgagggt cgaggctttt gcccgatact cggccgggtc
      541 caggtcatcg attcccgcac tgggtcggtc gatgatgttg gtgaggccca gacgccactc
      601 ggggaggcgc caatcctccc gaaaactcag tggttcgtcc accatgcctg agtcaaagag
      661 cagcttccag aagcggttag aatgaccagc gaagtgatga cccatcgctg cagatctcaa
      721 cccagggtta atgccgacaa agatgacatg caagtccggt cgtaaatggt cacgcagcct
      781 aggtctctgg ttgggcatta tggtcactcg ccccgggctg aggcagttta gctcgattca
      841 actattctgc cacagtttgg ccgagagatg agggcccccg tcgttcctgt agaacgcata
      901 agcgactgaa attgcaatga ggaagagggc ggatagaatc gggcaccaag cttgctgaat
      961 cctgacacga tgcccactga agggcataca ttccacaacc agaggaggtg acacagtatg
     1021 acacgcatgt tgttcgcaat tatggcagtg gcagtggcga tttctttcag cgctccttcc
     1081 ttcgccggtg aggagaagaa ggacaagaaa gatgagaaga agggcggcca cgtcctcgtg
     1141 tacggcgatg agaagaagga caagaaggac gagaagaagg gtggccacat ggacaccttc
     1201 ggggacgaga agaagaagga cgagaagaag ggcggaatgg gcggaaagta aatccctttt
     1261 cggcgaggct agctagctta aggagccttc cgccggaagg cggatggctc cttgggtgac
     1321 ctcatcactt tccgctttca gtaaatacca gctgtaatcc cacgattgtt ttcgcatcgc
     1381 gaattgttcc cgcgcgaatc atcccgattg cctcttccag cgaaacctcc accacctcga
     1441 ggacctcgtc tcgatcgaga tgttgccgcc ctttggtcag accggttgct ttgtacacat
     1501 gaatcacttc atcggtaaaa ccaggggccg tgaatatact cgagagaagc attagctggc
     1561 ctggcttata gccgatttct tcctccagct cccgcgtcgc acatgaacgg gggtcttccc
     1621 cggaatggag tttccctgcc gggatctcgt agataaatcc gcctgcggca tgccgaaact
     1681 gtcggatcag gacgaccgag ccatcttctg taatcgggac caccgccgcg gcacccgggt
     1741 gacgcaccac ttccaggtcg atcgtaaggc cattgggcaa ggtcaccgtc tcgatgttaa
     1801 gcgtgaccac tttgcctgtg tacaggttct tcgtcatgat caaggccgac aagctcggga
     1861 gacccgacga gggtcaaccg ttcttggttt ttatcttccg ataaaaggga ggcttcacaa
     1921 cggctgcggg cagccttttc ccccgaatat caatggaaat agccgtaccg gcctgggcaa
     1981 gggagggagg gacatacccc aagccgattc ctttctgaag cagtggcgaa aggttcccgc
     2041 tggtgacgac accgatcggt tgcgacgaca tcgcatctaa aatggtgaat ccatgacgcg
     2101 gtacggattt ctccaatact tcgaacgcga ccaaccgacg gggcgggcct tgtcgtgcct
     2161 ggtcgtcgag tgcccctttg ccgagaaagt ggcctttacg aaaattcact gtccattcgg
     2221 ctccagcctc gatcggcgtc gtctcctcgc tgatatcgtt cccgtacagc aggtaagcca
     2281 tctccagacg caacagatcc cgtgctccca aaccggccgg cttgaggccc ttgtattcgc
     2341 ctttcttgag taagaaggtc caaaggtgga tggcctgggt tgcaggaacg ttgagctcat
     2401 agcccaattc tccgctataa ccggttcgag cgacgaagca gttgatgtca ttcagggtga
     2461 tctcgcgaca gtgatgcagt tttaggtcac tgatcccttc cgctccggcc gtgaagagaa
     2521 tatcccgtga ggcgggacct tggatggcga tttgggcgag tgctgaagtc tgatcctcga
     2581 tgacacactg atcatgggtg gtggctcgat gctgcgagag ccacgccaaa attttctctc
     2641 gattcgaggc attgacacag aggagataat cggtttcgcc ggtgcgatac acaaagatat
     2701 cgtctttgat tccgcctgct tcgttgcata ccatggagta ctgagcctgg cagagtgcca
     2761 atgccgcaac gtcattggtg gtcattcgtt gaagaaaggc caggctgcca ccaccggaaa
     2821 ctctgatgcg tcccatatgg ctgacatcaa agaggccggc ctttgaacgg actgcctggt
     2881 actcatcgat cacgctggcg tattgaatgg gcatctgcca tccggcgaag tcgacaagtt
     2941 tggcgtggtg ggcacgatgt tggtcgatga gaggagtgga gttcattccg agaagggtgc
     3001 tgcgggtcag acagccagga gttccacttc aaagatgagc gtggcatgag gcggtatcgc
     3061 gtttccggct ccacgggcgc cataccccag atgggacggt atgatcaatt ttcgcttgcc
     3121 tccgacttgc atgccctgca cgccctcgtc ccatcccttg atgacccggc ccgcacccaa
     3181 tggaaaggaa aaaggctggc cacgctcaag tgagctgtcg aaaggcgtcc cattgtccag
     3241 ccagccgctg taatgcacac tcaccgtttg accggcttgt gccgtcgctc cggtgcccat
     3301 tctcaggtca atgtattgga gcccggatgc cgtcgtcact tctttaccct caggaccggc
     3361 ttgttcgaat ggcatggttt ccccttgtgt cgctggtgta aatcatttac tgaggttcat
     3421 gagcgtgatc gggacgcgag aacacggcga cacttgccga gtaaccatga aggaaatttc
     3481 ttcctcccac cggaccgatc tccccttgtg caaaaaagcc ggccactgga atcggtccca
     3541 tccgctcctg cacgacaccg ctgtcatgat gggcgcggcc gaagagccct tcccctctcc
     3601 cgcaacaact gaagaggagc gcacccaagg ctggattcgg gtgcttcgca cggtcggccg
     3661 cgagcaggac atgcaaatcg tcgctcgctg atttgccgtc gcggatttga aactgaatgg
     3721 tctggccttc ttgtacgaga tcgccgatcg cgagcgctcc cgattgttga tccgctccga
     3781 ccagattacg aacgagaaaa tctccgcgtt cgaaacggct gcgatgctca tcaatcgcga
     3841 tcccgatgtg taacgctcga tgggcatccc ggcgctgact cccttctagc gattcaaata
     3901 cttcctgtag ccgactcaga gccggttgcc ctccgagttc atagatcata ttttgttccg
     3961 ctctcgtgac cacataacgt tcgccgatgg gacagcagcc ttgtgatatc actggcctca
     4021 ccgcaatcgg acctgagagc tgtacgccga ccacgcctcc atcaaagact tctgcgttcc
     4081 tgatgaggcg attttcacca gggccatggc cgcctccaca caacccgccc aaggccagcc
     4141 cattagggta acggtcggtc atcaccgaaa gcacgtcatg aaccggtgtc gtgaacggat
     4201 cagccaacag gaggaacgtg ctcggttcag cgcttggctc aggccaccct gccatttgga
     4261 tttggtcctg cgtttgagag aaggccatgc ggagcggggt cacccggacc tgtggcagcg
     4321 aagccaccca aagagttaag gctggagcgg tttcaaattc ctcatggtcg gcgatcaccc
     4381 cttcgccgga acaaccgaca caaacttcaa tagacagttc ctcttgaatc atcgttgcca
     4441 cgagatcggc tttcttcgca tggtgaggag agaagaagac gaaagccaac tcgagggttg
     4501 ctcggccgag ttgtgcacga acggcatcag ccaggtcgcg tacggctgcc tcggtgtcag
     4561 tcttgtgcga gagagccgat gcaaaaagaa gtgcagtcga cgtgttgctc tggattgttt
     4621 gtgtcatcat gggtgtttct cgtggtcaca ctcctggatt cattcgctca gatcggtctt
     4681 tggcgagttt cttatagtag cgccacatgt aggagtggta gtcatccagt tgcgcaagca
     4741 ggtaatgcag ttccgtggga tcttccgttt cgagcgcccg aaccatcctt gttttcagga
     4801 attccccgaa ggcatcggtc ttttccacgg cggttaacaa acgcaagcca ccaccgatct
     4861 gatattcatt catccggtcc tccgatcgag atgcggcgtg agaatagcga gcagatggtt
     4921 caaaatgcaa gcgcttgaga gtaaccagca gttctatttg ctactggtgc cgagaatcgc
     4981 gcgcaggcga ctcaacgaaa tggcctccac tctgtagttg tcacgtccaa cgaccgttgt
     5041 ggccgccgtc aatgcgttga tgacagcctc ttccgtcgct tccacggtca cggtgatcac
     5101 agggttgaga tgtgtatctg caaggtgcgt gagggcatag gttcgctttt gaggataatg
     5161 ggggatcacg ttagcggttg agaatgcaat a
//
LOCUS       DASTQY010000012         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_198581, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000012 DASTQY010000000
VERSION     DASTQY010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198581"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFR82_00375"
     CDS             <1..196
                     /locus_tag="VFR82_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500080.1"
                     /translation="VRQMVGPTLRCFTFDKHLRDECRVLIPLLRFCAQDLAQSQGLDM
                     TQKLIVLDEIQAAIDLADLH"
     gene            264..1019
                     /locus_tag="VFR82_00380"
     CDS             264..1019
                     /locus_tag="VFR82_00380"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4500081.1"
                     /translation="MRLNVASCLVAGGVVTALIGCGLLETSKKSTTATTHARLQRQPA
                     TAVIGNSLPSQVEYTPTDAVKDTVADLLSILGNEALRQPDRSLDRRHFIEDIIRYRVS
                     YEDAAQRALGLQWKRLTDSERQEYVSLFMHLLRDMLANNIDQYYDEEMLYLSERRHDR
                     FAEVRTNLVGSKVNTSLYFRLGNQSGQWLIYDVVIDGTSLIRSYKAQFARIIRDDSYA
                     GLIEKMKQRNLLVKVFERTAPAVTLSSMHTAPQ"
     gene            complement(1559..2035)
                     /locus_tag="VFR82_00385"
     CDS             complement(1559..2035)
                     /locus_tag="VFR82_00385"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEU4500082.1"
                     /translation="MRRQSRRPISVLQSAGVVALILIALAGTVGSAQASQPGLHVEIL
                     GIRNSIGAVACALFEGPGGFPTEFLRFATNIMMVQVRATKATCDFADIAPGTYALAVI
                     HDENRDGELATNWMGKPQEGYGFSNDAKGTLGAPSFEAASFAYNGERLNMTIALHY"
BASE COUNT          492 a          593 c          560 g          516 t
ORIGIN      
        1 ggtgcgtcag atggtcggac ccaccctgcg ctgcttcacc ttcgataagc atttacgcga
       61 tgagtgccgc gtgttgattc cgctcctgag attctgcgcc caggatctcg cccaatcgca
      121 gggattggac atgacacaaa aattgatcgt cctcgacgag attcaagccg ccatcgattt
      181 ggcggatctc cactgacact cgaaaaatgg gaagtgagca attttgacca gtaggtcctc
      241 ctgtaaattg atagaatcgg ttcatgcgac tgaatgttgc ttcatgtctc gtggcaggcg
      301 gggtcgtaac agcgctgatc ggatgcggct tgctagagac ttcgaaaaag agcacgactg
      361 ccacaactca cgcccggctg caacgacaac cggcgacggc ggtcatcggg aacagcctcc
      421 cgagtcaagt cgagtacacc cccacggatg cggtcaagga tacggttgcc gatttgctgt
      481 ccattctcgg aaatgaagcg ctgaggcagc cggaccgatc tctggatcga cgtcacttca
      541 tcgaagacat catcagatat cgcgtcagtt atgaagacgc cgcgcaacgt gctttgggac
      601 ttcaatggaa gcgactgact gattcagagc ggcaagagta tgtgagcctt tttatgcact
      661 tattacgtga catgttagca aataacatcg accagtatta cgacgaggag atgctgtatc
      721 tttctgaacg acgccatgat cgctttgcgg aggtgagaac caacctcgtc ggatcaaagg
      781 tgaacacttc actgtacttc cggttaggga accagtccgg ccagtggctc atatacgacg
      841 ttgtcatcga tggcaccagt ttgatcagaa gctacaaggc gcaatttgct cggattatac
      901 gcgatgattc ctacgcgggc cttatagaaa agatgaagca gaggaatctt ctcgttaaag
      961 tgtttgagag gaccgcacct gccgtcaccc tgtcgtcgat gcacaccgct cctcagtaac
     1021 ggacctcttt cgacggtagc atcaattctc ggctcgcctc tcataggatc agcgatcttt
     1081 cgtagcaact ccggccaagg ttcatatgaa atactccctc cataccattc cggttgaagt
     1141 tcttgcactc gccttcattg agttggtctt cgtgagtacg tttgctgtcc tgctttggta
     1201 cgtgctcggt cacaaatagc cctggtattc ctcgtaaagg tttagcgtgg acaatttcat
     1261 tccatctgtt ttcctatggt tcgcccatca tcccttcggt gcggccacaa tggtgtatgc
     1321 gacaagcatc ctcggcgtct tgttgtatgc ctatttcgag cctagggacc atgtttaaga
     1381 gggcagtccc taagccgtat acgaggactc ccggatggat tctagctggg tgagcacttc
     1441 gcgtccgtcc aggcgtaggc tgtcaatcac gagatctcgg gaatgatcac ggaggaagga
     1501 cgacaataag gactgatcgt acaccagtgc gagcgcaagg gccgatggcg gcgttcgctc
     1561 agtagtgtag tgcaatcgtc atgtttaagc gttctccatt ataagcaaag cttgccgcct
     1621 caaacgaggg cgctcccaac gtgcctttcg catcattcga aaaaccgtat ccctcttgag
     1681 gcttccccat ccagttggtt gcgagttcgc cgtctcgatt ttcgtcatga atcaccgcaa
     1741 gcgcatatgt gccaggcgcg atgtctgcaa agtcgcaagt ggccttggtc gcgcggacct
     1801 ggaccatcat aatattggtt gcaaagcgta agaactcagt aggaaagcct cccggccctt
     1861 cgaacaaagc gcatgccaca gcgcctatgc tattccggat accgaggatt tccacgtgta
     1921 accccggctg agatgcctga gcggaaccga ccgtccccgc aagggcgatt aatatgagtg
     1981 ccaccacacc ggctgattgt agaacggata tcggcctgcg gctctgtctg cgcatgcgat
     2041 gacctgccag gtgaacaata ttgtcttgac cctcacctct ctggtccacc ctgtatagcc
     2101 ctagccacta tgacggtgtg gggccgccct ggtcgtcaat gcaccctgct ccctcaccaa
     2161 c
//
LOCUS       DASTQY010000013         3768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_996499, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000013 DASTQY010000000
VERSION     DASTQY010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996499"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..358)
                     /locus_tag="VFR82_00390"
     CDS             complement(119..358)
                     /locus_tag="VFR82_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HEU4500083.1"
                     /translation="MAHLRFHVRFPEDKVKEPIIYQIGREYNVVTNVRRADVRETTGW
                     VDVELSGETAEVERAIQGLRHKGCVVDPIELNVVE"
     gene            complement(358..633)
                     /locus_tag="VFR82_00395"
     CDS             complement(358..633)
                     /locus_tag="VFR82_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU4500084.1"
                     /translation="MIKVRIPTPLRPLTKGQGEVEVTADTIVDMIETLDTAHPGIKAR
                     LCDDSGELRRFVNIYVNEEDIRFLKGKDTSLKAGDEVSIVPAIAGGR"
     gene            complement(630..1880)
                     /gene="thrC"
                     /locus_tag="VFR82_00400"
     CDS             complement(630..1880)
                     /gene="thrC"
                     /locus_tag="VFR82_00400"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU4500085.1"
                     /translation="MAKMKALVCRECAKEYPTKAIHVCEMCFGPLEVKYNYAEVKQVI
                     SRKKIEEGPRSMWRYADLLPVEGTNYLGPHAGLTPLVRAKNLGAYLGIDELYIKNDTV
                     NHPTLSFKDRVVAVALTRARELGFETVACASTGNLANSVAAHAAAAGMRCYVFIPGDL
                     EAAKVLGNLIYKPNVVEVEGNYDDVNRLCSEIAAEHGWAFVNINVRPYYAEGSKTLAF
                     ETVEQLGWRTPDQVVIPMASGSLLTKIWKGLHEMKTVGLIDEVRTKINGAQAEGCSPI
                     STAFKAGRDFFKPVKPKTIAKSLAIGNPADGYYALKATGESQGSMDMVTDDEVVEGIK
                     LLAQTEGIFAETAGGVTIGVLQKLVKNGTIKKGDVTVAYVTGNGLKTQEAVVDAVGRP
                     VRILPSLVAFERTFRMGKNGGGDI"
     gene            complement(1893..2711)
                     /gene="moeB"
                     /locus_tag="VFR82_00405"
     CDS             complement(1893..2711)
                     /gene="moeB"
                     /locus_tag="VFR82_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEU4500086.1"
                     /translation="MEFTEEQISRYSRHILLPEVGGKGQKKIAKSKILLVGAGGLGSP
                     AALYLAAAGVGRIGLIDSDVVDLTNLQRQVLHHTADVGRPKVLSAKEKIQALNPDVSV
                     SMYEERLTAGNALKLFSDYDVIIDGVDNFTAKFLINDACFFADKPLVHGAILRFDGRV
                     TTILPRKSACYRCVFKKPPPPGLVASCQEAGVIGVLAGIIGTIQATEALKLVLGIGRP
                     LTDRLLDFDARKTQFREIKVKRNPQCALCGEHPTITELFDDGDPFAGCAVRPHS"
     gene            complement(2798..3715)
                     /gene="cysK"
                     /locus_tag="VFR82_00410"
     CDS             complement(2798..3715)
                     /gene="cysK"
                     /locus_tag="VFR82_00410"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU4500087.1"
                     /translation="MHKEITELIGGTPLVRLNRLSLPGSATIYGKVEFFNPGGSVKDR
                     ICLNMINEAERQGKLKPGGTIVEPTSGNTGIGLALIAAVRGYKLILVMPESMSMERAS
                     LLSSYGAQLVLTPAWEGMKGSIREAESLLMQNPSYFMPDQFSNPANPAMHKKTTAPEI
                     WEALDGKIDAFVAAVGTGGTITGCGEVFKERNPKVKVIAVEPAGSPVLSGGEPGPHKI
                     QGIGAGFIPKVLNRKILDRVIKVTDDEAYQTAKQLSKMEGLLVGISAGANVFAAQQVA
                     QELGPGKNIVTILCDTGERYISIEKYFNI"
BASE COUNT          758 a         1101 c         1006 g          903 t
ORIGIN      
        1 aactggccct tgccgccgac atcctgcagg atgatgtgac ggctgtaccg ctcgatctgt
       61 cgttcagtga gttccatgcc tcaattcacc ggttacaggg gcgcacgcca ggcgtgagtt
      121 attcgaccac gttcaattcg atcggatcaa cgacgcagcc tttgtgacgg agtccctgga
      181 tcgccctctc gacttccgcg gtctctccgg agagctcgac atcgacccat cccgttgttt
      241 ctcgcacatc ggcccgacgc acgttggtca ccacgttata ttcacgtccg atctgataaa
      301 tgatcggctc tttgaccttg tcttccggaa agcgaacatg aaagcggagg tgtgccatta
      361 tcgacctcct gcaatggcgg gaacaatcga cacttcatcg ccggctttga gcgacgtgtc
      421 ttttcccttc aaaaagcgaa tgtcttcctc attgacataa atattcacaa atctgcgcaa
      481 ttcgccgcta tcgtcgcaaa ggcgggcctt gatgccgggg tgagcggtgt ccagcgtttc
      541 aatcatgtcg acgatcgtgt cggcggtcac ttcgacttcg ccttgccctt tggtcaacgg
      601 ccgaagcggg gtggggatgc ggaccttgat catatgtcac caccgccgtt cttgcccatt
      661 ctgaatgtcc tctcaaacgc cacgaggctc ggcagaattc gaaccggccg accgacggcg
      721 tcgacgacgg cctcctgcgt cttcaatccg ttgcccgtca cgtatgcgac ggtcacgtcg
      781 ccttttttga tcgtcccgtt tttgaccaat ttctgaagca ccccgatcgt gacgccgcct
      841 gccgtttccg caaaaattcc ttcggtctgc gccaacagct tgatgccttc cacgacttcg
      901 tcgtccgtca ccatgtccat ggatccctga ctttcgcctg tcgctttcag ggcatagtac
      961 ccgtcggcgg ggttgccgat cgcgagtgac ttggcgatgg tcttaggctt taccggcttg
     1021 aagaagtcgc ggcccgcttt gaatgccgta gagatcggcg aacaaccttc cgcttgggcc
     1081 ccgttgattt tggtccgtac ctcgtcgatc aacccgaccg tcttcatctc gtgaagacct
     1141 ttccagatct tcgtcaacag ggaaccggag gccatgggaa tgacgacctg atcaggcgtg
     1201 cgccagccca gttgctcgac agtctcaaaa gcgagcgttt tggatccttc ggcatagtag
     1261 ggccgcacat tgatgttcac aaaggcccag ccgtgctcag cggcgatttc gctacatagg
     1321 cggtttacgt cgtcataatt gccttcgact tcgaccacgt tgggcttata aatcaggttg
     1381 cccagcacct tggcggcttc cagatcgcct ggaatgaata catagcagcg cataccggct
     1441 gcggccgcat gggcggcgac ggagttcgcc agattgcctg tggacgcaca agcgaccgtt
     1501 tcaaaaccga gttcgcgtgc ccgcgtcagc gcgaccgcga caactcgatc cttgaatgag
     1561 agggtcgggt gattgacggt gtcgttctta atatacagct cgtcaatccc taagtaggcg
     1621 ccgagatttt tggctcggac caagggagtc aatccggcat gcgggcctag atagttggtg
     1681 ccttcgaccg gaagcaagtc ggcatagcgc cacatgctgc gcggaccttc ctcaatcttt
     1741 ttgcgggaga tcacctgctt cacttctgca tagttatact tcacttcgag cggaccgaaa
     1801 cacatttcgc agacatgaat ggctttggtg ggatattcct tggcgcattc cctacacacg
     1861 agcgctttca tcttggccat cgtatcacca ccttatgaat ggggtcgcac ggcacaacct
     1921 gcgaacgggt cgccgtcgtc gaagagttca gtaatcgtcg gatgctctcc gcagagagcg
     1981 cattgaggat tgcgtttcac tttgatttcc ctgaactgcg tttttcgtgc atcgaaatcc
     2041 agaagccggt cggtcaatgg acggccgatc ccaagtacca gcttcaatgc ctctgtcgct
     2101 tgaatcgtgc caatgatacc tgcaagcacg ccgatgacac cagcttcttg gcacgaagcc
     2161 acaagcccgg gcgggggagg ctttttgaac acacaccgat aacaggcgga cttgcgaggg
     2221 agaatcgtgg tgacccgccc atcgaagcgc agaatggcgc catgtacaag cggtttgtcg
     2281 gcgaaaaaac aggcgtcgtt gatcagaaac ttggccgtaa agttatcgac gccgtcgatg
     2341 atcacgtcat agtcactgaa cagcttcagc gcattgcccg cggtcagccg ttcttcgtac
     2401 atcgatactg acacgtcggg attgagggct tgaattttct ctttcgccga gaggaccttg
     2461 ggacgcccga cgtcagctgt atggtgcagg acttgacgct gcagattcgt cagatcaacg
     2521 acatcactgt cgatcaaacc aatgcgtccg actcccgcgg cggccaggta gagggccgcc
     2581 ggcgaaccaa gccccccggc gccgaccagg agaattttgg acttggcgat tttcttctgg
     2641 ccctttccac cgacctcggg caagagaata tgccggctgt agcggcttat ttgctcttcg
     2701 gtaaattcca tatttcaacg cgatgccgaa atggtgtaca gcttcgtgcc ttgtttctta
     2761 cctgcgcttt gcaccatgaa aagcgcgtcc cggtttttca aatgttaaag tatttctcga
     2821 tgctgatata ccgctcgccg gtgtcgcaaa gaatcgtcac gatgttcttg cctgggccca
     2881 actcttgggc gacttgttga gcggcaaaaa cattggctcc tgccgagatc cccacgagga
     2941 gtccttccat cttcgacaat tgcttagccg tctggtacgc ttcgtcgtcc gtcaccttga
     3001 tgacgcgatc gagaattttg cgattcaaga ccttgggaat gaatccggcc ccgatccctt
     3061 ggattttgtg gggcccaggt tcaccgccgg aaagtaccgg agatcccgcg ggttcgactg
     3121 cgatgacttt gactttcggg tttcgttcct tgaatacctc gccacagccc gtgatcgtcc
     3181 cgccggtgcc gacggcagca acgaaggcgt caattttgcc gtccagcgct tcccaaatct
     3241 ccggagcagt ggtctttttg tgcatggcgg gattagccgg attcgaaaac tgatcgggca
     3301 tgaaataaga cggattctgc atcaaaaggc tttcggcttc tcggatggat cccttcatcc
     3361 cttcccaagc cggcgtcaac acgagttgtg caccgtagga ggaaagcagg ctcgcccgtt
     3421 ccatgctcat gctttccggc atcacgagga tcagcttgta gcccctgacg gcagcgatca
     3481 aggccagacc gatgcccgtg tttccactcg tcggctcaac gatagtgcca ccgggtttca
     3541 actttccctg ccgctccgcc tcgttgatca tgttgaggca gatccggtct ttgacgctgc
     3601 ctccgggatt gaagaactcc accttcccgt aaatcgtagc gcttccaggc agggagaggc
     3661 ggttgaggcg gacgagggga gttccgccga tcagctctgt aatctctttg tgcatgacca
     3721 tggtcatttg ccgccaccca tgtagaacag aatttcaagc cggtcgcc
//
LOCUS       DASTQY010000014         6052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1600069, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000014 DASTQY010000000
VERSION     DASTQY010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600069"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /gene="rpsE"
                     /locus_tag="VFR82_00415"
     CDS             <1..198
                     /gene="rpsE"
                     /locus_tag="VFR82_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247903.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU4500088.1"
                     /translation="GIIAGGAVRAVVELVGAHNIISKTLGRGNPFNTVRATLDGLTQL
                     RHPDEVLRLRRGEELQTGALA"
     gene            198..404
                     /gene="rpmD"
                     /locus_tag="VFR82_00420"
     CDS             198..404
                     /gene="rpmD"
                     /locus_tag="VFR82_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU4500089.1"
                     /translation="MATAKPPKTAAHVRITLKKSPIGTPERHRLVLRGLGLRKIRQSV
                     VRPDTPQVRGLIHQVGYLLEVQPS"
     gene            401..850
                     /gene="rplO"
                     /locus_tag="VFR82_00425"
     CDS             401..850
                     /gene="rplO"
                     /locus_tag="VFR82_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU4500090.1"
                     /translation="MKLHDLAPAKGAKKKRKRIGRGPGSGHGKTATKGHKGLLARSGG
                     GKQPGFEGGQMPLIRRVPKYGFTNPFRKEYAIVNIERLADLPASGTITPQAMVDAGLV
                     KRVTLPIKILGNGELKKAITVQAHKFSKSAEAKIQAAGGSVEVIPGV"
     gene            843..2162
                     /gene="secY"
                     /locus_tag="VFR82_00430"
     CDS             843..2162
                     /gene="secY"
                     /locus_tag="VFR82_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU4500091.1"
                     /translation="MFERLLTSFQNILKIPELRTRILFTLGMLVVYRVGAHIPTPGIN
                     GEALSEFLQKQGGALLGFLDIFSGGSLSRLTIFALGIMPYISASIILQLLTVVVPHLS
                     KLAKEGERGRKKIIQYTRFGTIGIALVQGFGIAIGLEQMNGGAFVLDKGWAFRLMTVI
                     TLTAGTGFLMWLGEQITERGIGNGISLIIFAGIVARLPSAVAQTYNLYEIGQLNAFLL
                     VGLAALMVAVVAAIVFLESGRRKVPVQYAKRVVGRRVYGGQSTHIPLKINTAGVIPPI
                     FASSIIAFPATIAGFFETPWIKAIGAQLSPGSLLYTLMYVGLILFFCFFYTAVVLNPV
                     DMADNMKKYGGFIPGIRPGQRTSDYIYKVLTHITFAGALYLAIVCVIPEFLIYKLNVP
                     FYFGGTSLLIVIGVGLDTAQQIESHMLMRNYEGFLGKGMGPLRGRSG"
     gene            2170..2826
                     /locus_tag="VFR82_00435"
     CDS             2170..2826
                     /locus_tag="VFR82_00435"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU4500092.1"
                     /translation="MRVVFLGAPGVGKGTQADRIAAQFKIAKISTGDLLREAVRNQTP
                     LGREAKQFMDQGRLVPDSVVIGLVRDKLAEPACANGFVLDGFPRTVPQAEELEQVLRA
                     PRRPLNRVVNFKVPREVVIKRLSGRRSCPKCQATFHVEFAQSQKGTQCDRCGETLVQR
                     SDDKPEAIETRLKVYEEQTAPLIQYYANKRLLSELDASGSVDAVFEALFGVLAPYSAL
                     "
     gene            2823..3572
                     /gene="map"
                     /locus_tag="VFR82_00440"
     CDS             2823..3572
                     /gene="map"
                     /locus_tag="VFR82_00440"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEU4500093.1"
                     /translation="MIILKTPDEIAVMAQASKVVAEALEVLKRAVKPGVTTDELDRLA
                     EAEIRGRGAIPAFKGYRNYPKTLCASVNEQVVHGIPSKRVLKDGDIVGLDLGAIVGGF
                     YGDSAVTVAVGRIDEKAATLVRVTEESLSLAIQQARVGNRLSDISHAVQQHVEAAGYS
                     VVTEFVGHGIGRQLHEEPQVPNYGKPGQGPRLQPGMVLAIEPMVNMGGSAVRVLDDRW
                     TAVTADGSLSAHFEHTIAIQPSGPALVLSRL"
     gene            3613..3831
                     /gene="infA"
                     /locus_tag="VFR82_00445"
     CDS             3613..3831
                     /gene="infA"
                     /locus_tag="VFR82_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU4500094.1"
                     /translation="MPKEDIIEIQGSVLETLPNAMFRVQLDNGHKILAHISGKMRMHF
                     IRILPGDKVTVEMSPYDLTRGRITYRFK"
     gene            3844..3957
                     /gene="rpmJ"
                     /locus_tag="VFR82_00450"
     CDS             3844..3957
                     /gene="rpmJ"
                     /locus_tag="VFR82_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU4500095.1"
                     /translation="MKVKSSVKPICAKCKVVRRRGVVRILCVNPRHKQRQG"
     gene            4131..4526
                     /gene="rpsM"
                     /locus_tag="VFR82_00455"
     CDS             4131..4526
                     /gene="rpsM"
                     /locus_tag="VFR82_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU4500096.1"
                     /translation="MARIAGVDLPREKRSDIGLTYVFGIGRAAARNILDKAGIDGAIR
                     VKDLSEDQIVKIREIIDQEYQVEGDLRKTFSMNIKRLIDTGTYRGLRHRKGLPVRGQR
                     SKTNARTRKGRRAGVGSKPKPPARPAPTA"
     gene            4545..4928
                     /gene="rpsK"
                     /locus_tag="VFR82_00460"
     CDS             4545..4928
                     /gene="rpsK"
                     /locus_tag="VFR82_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU4500097.1"
                     /translation="MSIKKGKKKERRIVQSGIAHVQASFNNTIVTITDMSGNTVVWAS
                     AGNQGFKGSRKSTPFAAQRAGEAAARKAMESGMRQVDVYVNGPGSGRESAIRSLQGAG
                     LRINLIRDVTPIPHNGCRPPKRRRV"
     gene            5006..5632
                     /gene="rpsD"
                     /locus_tag="VFR82_00465"
     CDS             5006..5632
                     /gene="rpsD"
                     /locus_tag="VFR82_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU4500098.1"
                     /translation="MAKYRGPVCRLCRREGEKLFLKGSRCMTEKCAIERRSYPPGQHG
                     QARPRISDYSTQLREKQKLRRIYGLQERQFRGVFERAERQSGVTGDALLRLLECRLDN
                     VAYRLGFGASRKEARQIVGHGHVTVNGRKNNIPGAQVRVGDVVAVRERSRNMVSIQTA
                     LESVEGRGIPEWLDLDKAGFKGTVRALPTKDQITLPVNEQMVVELYSR"
     gene            5711..>6052
                     /locus_tag="VFR82_00470"
     CDS             5711..>6052
                     /locus_tag="VFR82_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU4500099.1"
                     /translation="MIKAMKDFQIPMRVEVDKETASTTFGRFTTEAYERGFGTTVGNA
                     LRRVLLSSLTGAAVTTVKIEGVLHEFSTIPGVTEDVTSIILNIKGLRLASHTDKPKTI
                     RLKKKGPGEAKG"
BASE COUNT         1329 a         1555 c         1967 g         1201 t
ORIGIN      
        1 ggcattatcg ccggcggagc ggtccgagcg gtcgttgagt tggtgggagc gcacaacatc
       61 atttccaaaa cgttgggccg aggaaacccg ttcaacacgg tacgcgcgac cctcgacggt
      121 ttgacacagc tcagacatcc cgatgaggtc ttgcgattgc gtcggggcga ggaacttcag
      181 acgggagctc tggcgtaatg gcaacggcaa aaccacccaa gacggcggct catgtgcgca
      241 tcacgttgaa gaaaagtccc atcggcacgc ctgagcggca ccgcctcgtc ctgcgcggac
      301 taggattacg caagatccgt cagtcggtgg tccgtccgga tacgccgcag gtacggggat
      361 tgattcacca agtgggctat cttctggaag tgcagccatc atgaaactac acgatctcgc
      421 cccggctaaa ggggccaaga aaaaacggaa gcgtattgga cgtggccccg gctcgggtca
      481 tgggaagacg gcaacgaaag ggcataaggg attgctggct cgctcaggag gcggcaagca
      541 gcctgggttc gaaggcggcc agatgcctct cattcgtcgc gtccctaagt acggctttac
      601 caatcctttc cggaaagaat acgcaatcgt caacatcgag cgtctggctg atctgccagc
      661 ttcggggact attacgccgc aggcgatggt cgacgcgggt ctggtcaagc gggttacatt
      721 gccgatcaag atcttgggca atggtgagtt gaagaaagcc atcaccgtgc aggcgcacaa
      781 gttcagcaag tcggctgagg cgaagatcca agcagcggga gggtcagtcg aggttatccc
      841 cggtgtttga gcggctcctg accagctttc agaatattct gaaaattccc gagctgcgga
      901 cacgcatcct gtttacgctg gggatgctgg tggtgtatcg ggtcggcgca cacatcccga
      961 cacctggtat caacggcgaa gcgctctctg agttcttgca gaagcaggga ggagcgcttc
     1021 ttggcttcct cgatattttt tcagggggtt cgctgtcgag gctgacgatc tttgcgctgg
     1081 gcatcatgcc ctatatcagc gcatcgatca tcttgcagct gttgacggtg gtggttcccc
     1141 atctctcgaa gctcgccaag gagggagaac ggggtcgcaa gaaaattatt cagtacacgc
     1201 gtttcggcac catcgggatc gcgttggttc agggtttcgg gatagccatc ggtctcgaac
     1261 agatgaacgg cggcgcgttt gtcttagaca agggctgggc gttccggctg atgaccgtga
     1321 tcacactgac ggccgggacg gggttcttga tgtggctggg cgagcagatt acggagcgcg
     1381 gcatcggcaa cgggatctcc ctgattatct ttgccggcat cgtggcgcgt ctgccttcgg
     1441 ccgtggcgca gacctataac ttgtatgaga tcggccaact caacgcattc ttgctggtcg
     1501 gccttgcggc gctgatggtg gccgtcgtgg ccgcaattgt gttcctagaa agcggacggc
     1561 gcaaagttcc ggtgcaatac gcgaagcggg tggtgggtcg ccgggtctat ggcggtcaga
     1621 gcacccatat tccattgaaa atcaatacag cgggtgtgat cccgccgatt tttgcatcat
     1681 cgatcattgc gtttccggcg accatcgcgg gattttttga aacaccctgg atcaaggcga
     1741 tcggtgcgca attgtcgcca ggatccctgc tctatacgct gatgtatgtt ggtctgattc
     1801 tgtttttctg ttttttttac acggcagtgg tcctcaaccc ggtggacatg gccgacaaca
     1861 tgaagaaata cggagggttc attcccggca tccggccggg gcagaggaca tccgattaca
     1921 tctataaggt gctgacacac attacgtttg ccggggcatt gtatctggcc atcgtctgtg
     1981 tcattcctga atttttgatt tacaagctga acgtcccctt ttatttcggc gggacttcgt
     2041 tgttgatcgt cattggcgtc ggcttggata cggctcagca gatcgaatcg cacatgttga
     2101 tgcgcaacta cgaaggattc ttgggtaagg gcatgggtcc gctccgcggc cggagcgggt
     2161 gaggaccgca tgcgtgtcgt gtttctcggt gcgccgggag ttggtaaagg gacgcaggcg
     2221 gatcggatcg cggctcaatt caagattgcc aagatttcga cgggcgactt gttgcgtgag
     2281 gcggttcgca atcagacccc gttagggcgt gaggcaaaac agttcatgga ccagggcagg
     2341 ctggtccccg attcggttgt cataggcctc gttcgagaca agcttgccga gcccgcttgc
     2401 gcaaacggat tcgtgctgga cgggtttccg cgaaccgttc cccaggctga agaattggaa
     2461 caggtcctgc gggctccccg gcggcctctc aatcgggtcg tgaacttcaa ggtgccgcgt
     2521 gaagttgtca tcaagcggtt gagcgggcgc cgcagttgcc cgaaatgtca ggcgaccttc
     2581 cacgtggagt tcgcccagtc ccagaagggt actcagtgtg atcggtgcgg cgagaccttg
     2641 gttcagcgga gcgacgacaa gcccgaagcg atcgaaacac ggttgaaggt ttacgaagaa
     2701 caaacggcgc cgttgatcca gtactatgcc aacaagcggc tcctctcgga actggatgcg
     2761 tccggttccg ttgatgcggt gtttgaagcc ctgttcggcg tcctggcgcc ctacagcgcg
     2821 ctatgattat tctcaagacg ccagacgaga ttgccgtgat ggcacaggcg tcaaaagtag
     2881 tggcagaggc gctggaagta ctgaaacgcg ccgtcaagcc aggcgtgacg acggatgaat
     2941 tggaccggtt ggcggaggcc gaaattcgcg gcagaggcgc aattccggcg ttcaaagggt
     3001 accggaatta tccgaagacg ctttgcgcgt cggtcaatga gcaggtcgta cacggcattc
     3061 cctccaagcg ggtgctgaag gacggcgata ttgtcgggtt ggatttgggc gcaatcgtcg
     3121 gcggattcta cggcgattcg gcggtgaccg tggccgtcgg gcgaattgac gagaaagcag
     3181 cgacgctggt tcgagtaacg gaagaatcgc tgtcgctcgc gatccagcag gcgcgggttg
     3241 ggaaccggct gtcggatatt tctcatgcgg ttcagcagca tgtcgaggcg gccggctatt
     3301 cggtcgtcac cgagtttgtc ggacacggga tcgggcgcca gctgcacgag gagccgcagg
     3361 tgccgaacta cggcaagcca ggacagggtc ctcggctcca gcccggcatg gtgttggcga
     3421 ttgaaccgat ggtgaacatg gggggatcgg ctgtgcgggt gttggacgac cgctggacgg
     3481 cggtgactgc ggacggcagc ctctcggctc actttgaaca tacgattgcg attcaaccga
     3541 gtggtccggc actcgtcctg agtcggttat agcacagcgg gcgtgactca ccgggagaaa
     3601 gggtaacggt tcgtgccgaa ggaagacatc atcgagatcc aaggttcggt actggagacg
     3661 ctgcccaacg ccatgtttcg cgtgcaactc gacaacggtc ataagattct cgcgcacatt
     3721 tccgggaaaa tgcgcatgca cttcatccgc attcttccgg gggacaaggt cacggtggaa
     3781 atgtcgccgt atgatctgac ccgtgggcgc attacgtatc ggttcaagta ggagctgtcg
     3841 tccatgaaag tgaaatcatc ggtcaagccg atttgtgcca agtgtaaagt cgtgagacgc
     3901 cgtggggtgg ttcgaatact gtgtgtcaat ccacggcaca aacaacggca ggggtagtgg
     3961 ggcggctggg aggctggcga gtgtcctagc gcgccatcgc gagcaatgag gaagcggcgt
     4021 cttggcgccg cagggctggc gggtgagaag gcggcgatcg ttcaacgcgc gcagcggagg
     4081 cgcatccagc ctcggccgcg gaagacgttg aagcggaagt aggaggaaac atggcacgta
     4141 tcgcgggggt agatttgccg agagaaaaac ggtcggacat cggcctgacc tatgtgttcg
     4201 ggatcggcag ggcggccgcc aggaatattt tggacaaggc cgggatcgat ggcgccatcc
     4261 gggtcaaaga tcttagtgaa gaccagatcg tcaagatccg cgagattatc gatcaggaat
     4321 accaggtcga gggcgatctc agaaaaactt tctcgatgaa tatcaagcgc ctgatcgata
     4381 cgggaacata tcgcggcctt cgtcatcgta aagggttgcc cgtccgcggc cagcgttcga
     4441 aaaccaatgc gcgcacacga aaggggcgtc gtgccggagt cggcagcaag ccaaaaccgc
     4501 ccgcacgtcc agcgccgacc gcatagtcat tgtaggagtc tgtcatgagc ataaaaaaag
     4561 gcaagaagaa agaacgacgg atcgtccaaa gcggcatcgc ccatgtccag gcctcgttca
     4621 ataacaccat cgtgaccatc acagacatga gcggcaatac ggtggtatgg gcgagcgccg
     4681 gcaaccaagg cttcaaagga tcgcgtaaaa gcacgccgtt tgccgcgcag agggccgggg
     4741 aggctgccgc aagaaaggcg atggaaagcg gcatgcgcca ggtggacgtg tacgtaaacg
     4801 gcccgggatc gggacgcgag tcggcgatac gttcattgca gggggcggga ctgcggatta
     4861 atctgatccg tgacgtgacg ccaatcccgc ataatggctg caggcctccg aagcggcggc
     4921 gagtctaatg aaaaatgcac agaggtgaat cggaacgaag cgggcagacc atgaagggcg
     4981 tgcccgaaaa ccggaggtag agcaagtggc aaagtatcgt ggtccagtct gtcggctgtg
     5041 tcgtcgtgaa ggcgagaagt tgttcttaaa aggctcgcgt tgcatgaccg aaaaatgtgc
     5101 cattgagcgt cggagctatc cgcccggcca gcatggccag gcccgcccaa gaatttcaga
     5161 ctacagcacc cagttgcggg agaaacagaa gctgcggcgg atatacgggc ttcaagaaag
     5221 gcagttccgg ggtgtgtttg aacgggcgga acggcagtcg ggtgtgacgg gcgacgcctt
     5281 gctgaggctg ctcgaatgtc gcctcgataa cgtggcctac cgactggggt tcggggcgtc
     5341 gcgcaaggaa gcccgccaaa ttgtcggcca tgggcacgtg acggtcaatg gccggaagaa
     5401 caatatcccg ggggcgcaag tccgtgtcgg tgatgtagtg gcggtacggg agcgaagccg
     5461 gaacatggta tcgatccaga cggcgttaga gtcggtcgaa ggacgaggca ttccggaatg
     5521 gctggatctc gataaggccg gcttcaaggg caccgtgcgg gcgttaccaa ccaaggacca
     5581 gatcacgctg ccggtcaacg aacagatggt cgtcgaattg tactcgcgat aacggaaact
     5641 tcatacaacg ccggctttgt gcagagtgca tcgcagaaag tgcggcggct gacaggatga
     5701 agggggactc atgattaaag cgatgaaaga ttttcaaatc ccgatgcggg ttgaagtgga
     5761 caaggaaacc gcttccacca ccttcgggag attcaccacg gaagcctacg agcgtggctt
     5821 cggaacgacc gtcggcaatg ccctgcgccg tgtgctgctc tcatccctga ccggagcggc
     5881 ggtgacgacc gttaagatcg aaggcgtgct gcacgagttt tcgacgattc ctggtgtgac
     5941 ggaagacgtc acgtcgatca ttctgaacat caagggatta cggttggcct cccacacgga
     6001 caagccgaaa acgatccggt tgaaaaagaa aggccctggc gaagccaagg gg
//
LOCUS       DASTQY010000015         4078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1802974, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000015 DASTQY010000000
VERSION     DASTQY010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802974"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFR82_00475"
     CDS             <1..495
                     /locus_tag="VFR82_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEU4500100.1"
                     /translation="EIELVSVEEREKLLRSFLNGVESLFDIIFLDCPPALGILTVNAL
                     VAARSVLIPVQCEYYAMEGLSRLIGSIERIQQSWNPGLTIEGIVLTMYDPRNTLARQV
                     AEQVRGHFKDLVYQTTIPRNVTLAEAPSYGRPVLLYNMASAGAQAYQSLAKEFINHGE
                     KGPR"
     gene            473..1321
                     /locus_tag="VFR82_00480"
     CDS             473..1321
                     /locus_tag="VFR82_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU4500101.1"
                     /translation="MEKKALGRGLDALLPTTKPAPMPELPEVQHLRIDAIVPNRYQPR
                     QTFPPQELAELAASLKQSGLLQPVLVRRKGDGMYELISGERRWRAAREAGLETIQAMI
                     RNCSDEESILLALVENLQREDLNPIEMARAYQRMMNEFGLTQDTVAQRVGCERSSVAN
                     AVRLLQLPIEVQHLIETDQLSAGHAKVILGLASPAEQLRVAHLVVSRNLSVRETEQIL
                     SSGLIVRKRNAKELRRSPFSDVEERLQKRLGTKVSVLNGRRGGKVIIHYFSPAELDGL
                     LEQLLQ"
     gene            1481..1960
                     /locus_tag="VFR82_00485"
     CDS             1481..1960
                     /locus_tag="VFR82_00485"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU4500102.1"
                     /translation="MWGQDKQDGKRAVEGDNEEGAEPVSRASEETEDIIAFVGKGVEF
                     KGVISYSGTVRIDGFLDGEIHTDGVLLVGDEAVIQAKVTAGTIVCKGKITGDIAAKER
                     VKLRAPAVFNGSMKTPMLSIEDGVLFNGGLEMSQGMRDGTALHPVGATSPAAVKRVS"
     gene            2007..2405
                     /locus_tag="VFR82_00490"
     CDS             2007..2405
                     /locus_tag="VFR82_00490"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU4500103.1"
                     /translation="MWARKDQVEQIEPDFENFTLLAKDVDFKGVVNFDGTVRVDGRVE
                     GEIHTTGTLIVGEHAMIKGIVSAGVLMNSGKINGTITAVEKIHILKPGVLVGDIRTPS
                     IAIEEGSHFHGMCDMGVQSWSEPAAVSIAS"
     gene            2493..3560
                     /gene="mnmA"
                     /locus_tag="VFR82_00495"
     CDS             2493..3560
                     /gene="mnmA"
                     /locus_tag="VFR82_00495"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU4500104.1"
                     /translation="MLLGMSGGVDSSVAAALLVRDGYEVQGVTLQVWEHEDDSVAASK
                     KWQERGCCKIGIAKYVAQRLKISYEVIDQRQNFRESVIKDFLDGYAGGTTPNPCVRCN
                     ERVKLRGLIDLAEQRGIEYVATGHYVRVREHGGQYALHRALDEKKDQSYFLYRLNPAW
                     LPRLRFPVGGMQKSDVWSEAEALGRPVDELKESQEICFVSHGDYRTFIESEMPETRRP
                     GDFIDNNGRYLGRHDGIAFYTPGQRRGLGIAAGQRLYVQQVMPLTNTVVLGPEEALLS
                     SACHVQELNLFDRSLCAGKADVAVKVRYATPATAATVEPIDHDRLLLTFRAPQRALSP
                     GQSAVLYHGDRVLGGGMIQAI"
     gene            3689..>4078
                     /gene="atpH"
                     /locus_tag="VFR82_00500"
     CDS             3689..>4078
                     /gene="atpH"
                     /locus_tag="VFR82_00500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEU4500105.1"
                     /translation="MIKTAVARRYAQALFELLDQSNIEAMRRTLHGLGQAMKQSSSLR
                     HVVSSPAFGAEEKIVVLTELGERLGCPPTGKAFFGQLVKKNRVGFLPDIAEAFAKLVD
                     QSKGMQQVTVSSAVALPQAEQDRIKGRL"
BASE COUNT          922 a         1094 c         1181 g          881 t
ORIGIN      
        1 gaaatcgaac tcgtcagtgt tgaggagcgc gagaagctat tgcgcagctt tcttaacggg
       61 gtagagtcct tgtttgacat catcttcctt gattgccctc ctgcattagg gatattgacc
      121 gtcaacgctc tcgtggctgc tcgctcggtg ctcatacccg tgcagtgcga atattatgcg
      181 atggaaggcc taagtcgatt gatcggcagt atcgaacgga ttcagcagtc gtggaatccc
      241 ggcctcacga tcgagggcat cgtcttgaca atgtacgacc cgaggaatac gttggcgcgc
      301 caagttgccg agcaggtacg tggacacttc aaagacctcg tgtatcaaac gaccatccca
      361 cgaaacgtga cacttgcaga ggctccaagc tacggccgtc cggttctgct ctacaacatg
      421 gcctcagccg gtgcacaggc ctaccaatct ttagccaagg agtttatcaa tcatggagaa
      481 aaaggccctc ggtaggggac tcgacgcgtt gttgccgacg acgaaacctg ctcccatgcc
      541 ggagttgccg gaagtgcagc atcttcgaat cgacgccatt gtgccgaatc gctaccaacc
      601 gcgccaaacg tttcctcccc aagaattggc cgagcttgcg gcatcactca agcaaagcgg
      661 attacttcag cccgtgcttg tccggcgaaa aggcgatggc atgtatgaat tgatttcggg
      721 cgagcggcgt tggagggccg ccagagaggc tgggcttgaa accattcaag cgatgatacg
      781 gaactgtagt gatgaagaat ccatcctctt ggcactggtc gaaaatctgc aacgagaaga
      841 cctcaatccg atcgaaatgg ctcgagcgta tcaacgcatg atgaatgaat ttggtcttac
      901 gcaggacact gttgcgcagc gcgtcggctg cgagcgctca tcggtcgcga atgctgttcg
      961 tcttctccag ctcccgatcg aggtccaaca cctgattgag accgatcaat tgtctgccgg
     1021 tcatgcgaag gtcatcttgg ggctcgcgag ccctgctgaa caactccgcg tggcgcatct
     1081 cgtcgtcagc cgtaatctct ccgtccgtga gacagaacaa attctttcat ccggcctaat
     1141 cgtcaggaaa cgcaatgcaa aggagctacg ccgctcaccg ttctctgatg ttgaagaacg
     1201 tttgcaaaaa cggcttggaa cgaaagtgtc cgttctcaat ggaagacgcg gaggaaaagt
     1261 gatcattcac tacttttctc cagccgagct cgacggtctc ctcgagcagc tgcttcagta
     1321 gttcccctct acgtaccccg gcacgaccct tcggacgtaa tctgcatatt tgttgctaaa
     1381 tcgccagacc ggtggcagtg tttgttcgta cgattaatgc tgctcactca cagcgatccg
     1441 cgacgagaag aggcggagag aattctgggg aggatgagtc atgtggggcc aggacaaaca
     1501 agacggcaag cgtgctgtcg aaggtgacaa cgaggagggc gccgaaccag tcagtcgagc
     1561 aagcgaggag accgaggata tcatcgcctt tgtcggcaaa ggagtcgaat tcaagggcgt
     1621 catctcctac agcggtaccg tcaggatcga tggcttcctc gatggcgaaa tccatactga
     1681 cggcgtgttg ctagtgggcg atgaggcggt gatccaagcg aaggtcaccg cgggcacgat
     1741 cgtctgcaag ggcaagatca caggagacat tgctgcaaaa gaacgggtca agctgcgggc
     1801 tcctgctgtg ttcaatggga gcatgaaaac gccgatgctg tcgatcgaag acggcgtttt
     1861 attcaatggc ggccttgaga tgagccaagg gatgcgggac ggaaccgcac tccacccggt
     1921 cggcgcgact tcgcctgcgg ctgtgaagcg tgtgagttga taagcagatt acctaggtaa
     1981 atataccaat acaaagcgga ggatttatgt gggcaagaaa ggaccaagtc gagcagatag
     2041 agccagattt tgaaaacttt acgctgctgg cgaaagatgt cgattttaag ggtgtggtca
     2101 attttgatgg aacggtccgg gtcgatggcc gagtggaagg ggaaattcac accaccggca
     2161 cactgattgt gggcgagcat gccatgatca agggcattgt gtcggcaggc gtcctcatga
     2221 acagtggaaa gatcaacggc acgatcacag ccgtcgaaaa aattcatatt cttaagccgg
     2281 gcgtcttggt gggggatatt cgaacgccgt cgatagcgat tgaggaaggg tcgcatttcc
     2341 acggcatgtg cgacatgggt gtgcaatcgt ggtccgagcc cgcagcggtc tcgatcgctt
     2401 cctagtccag gccgactcac ctctccgttg atagacactg gccatttttc ctctcctgcc
     2461 ggtacaatac cgtcacaatg aagaagccca cggtgttgct cggaatgagc ggcggcgtcg
     2521 acagttcggt ggcggcagct ctcctcgtac gtgacgggta tgaggtgcag ggggtcaccc
     2581 ttcaagtctg ggaacacgaa gacgattccg tcgcggcgtc caagaaatgg caggagcgcg
     2641 gttgttgcaa gatcggcatc gctaaatatg tcgcacagcg tctcaaaatt tcttatgagg
     2701 tcatcgatca gcggcaaaac tttcgggaga gtgtcatcaa ggatttcctt gacggctatg
     2761 ccggagggac cacgccgaat ccttgcgtcc gctgcaacga acgcgtgaag ctgcgagggc
     2821 tcatcgacct cgcggaacag cgcggaattg aatacgtggc gacgggacat tacgttcggg
     2881 tccgggagca cggtggtcag tatgctctcc accgcgctct ggacgagaaa aaggaccaga
     2941 gttatttcct ctatcgtctt aatcccgcct ggcttccccg ccttcgattt ccagttggcg
     3001 gcatgcagaa gtccgacgta tggtcagagg cagaggccct cggacgtccc gtcgatgaat
     3061 taaaggaaag ccaggagatt tgctttgtga gccatggtga ttatcgcacc ttcatcgaat
     3121 cggaaatgcc ggagacaaga cgccctggcg atttcatcga taacaatggg cgctatctgg
     3181 gacgccacga cgggatcgct ttttacacgc cgggccagcg acgcggactc ggcattgcgg
     3241 ccggtcaacg tctctacgtg caacaggtta tgccgttgac caataccgtc gtcctcggtc
     3301 ccgaagaggc cttgctcagt tccgcatgtc atgtacagga gctcaatctc tttgatcgta
     3361 gtttgtgtgc cggcaaggcg gatgttgccg tgaaggttcg gtatgccaca ccggcaaccg
     3421 cagcgacggt tgaacccatc gaccacgaca ggttgctcct cacctttcgt gcgccgcagc
     3481 gagccctgag ccccggtcaa tccgccgtgc tctaccatgg tgatcgggtg ctcggcggcg
     3541 ggatgatcca agccatctga ctgccttcgt ctcggcccgt ctgtattgac acttcccatc
     3601 cgccaatgct aggtttgcgc gcccattttc gtgcctcctg cacggaagta tcccgtttgc
     3661 cttaaatacc aaagagcgga ttaggacagt gatcaagaca gcagttgcgc gacgttacgc
     3721 ccaggccctc ttcgagctcc tcgatcaatc gaacatcgaa gcgatgcggc gtacccttca
     3781 tggtctgggc caggccatga agcagtcgtc ttctctccgg catgtcgtct cgtctcctgc
     3841 attcggtgcc gaggagaaga tcgtcgtcct cacagagctt ggggagcgtc tcggttgtcc
     3901 gccgaccggc aaggccttct ttgggcaact ggtcaagaag aaccgagtcg ggttccttcc
     3961 cgatattgca gaggcatttg ccaaactggt cgatcagtcg aaaggaatgc agcaagtcac
     4021 ggtgtcttcg gcagtggcgt tgccccaagc agaacaggac cgcatcaagg ggcgcctg
//
LOCUS       DASTQY010000016        22160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1398666, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000016 DASTQY010000000
VERSION     DASTQY010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 22160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22160
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398666"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..1539
                     /locus_tag="VFR82_00505"
     CDS             382..1539
                     /locus_tag="VFR82_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU4500106.1"
                     /translation="MAEPSSIRFHNRLKDVRVASRLSQGDLASKAGITRQAVSAIESE
                     LYLPTTAVALRLAAVLSCRVEDLFSLAASEEVVEGRYIGGQDKSPTVPLRVKVTKVGK
                     NIIVRPVAELGEVLAYTVPADGYLLETTTAKSGATARVRLSRDREAIDEEISVAGCDP
                     AIFLAGAHLRRHKDRTSVIGWSMGSMAAVGALQRSEVHVAGLHLFDPLSGESNLPFLK
                     RALKGSDYEVVTFATWEEGFLVRPGNPKAIRQVADLADETVIFVNREEGSGARLLLDQ
                     RLRAAGVDHARVRGYENTARSHFDVARTIAGRQADVGIGIRSAAQSFGLGFVPLQSAR
                     YDLVVAKSILQSHPSLSNLFDAISSRRFREEIEALGGYDTRETGKLHALRS"
     gene            1567..2394
                     /gene="modA"
                     /locus_tag="VFR82_00510"
     CDS             1567..2394
                     /gene="modA"
                     /locus_tag="VFR82_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4500107.1"
                     /translation="MRPCVLLYGLFLIVWIMTASAYATESLIIATSPSVAPAVEALGR
                     QFETTHPDVRVRLYLDSGLNLRRTIAGMENNPRGQYFIGSGPIHLVAPGGDELLTRLE
                     QKYYVLPGTRRPYAEVPLVMVVPEALVDAPTSFESLTQNKTLRIAVGDPALTMLGQRT
                     MQLLTTLGIWAAVENRLDRASDVRSVLDHVLNGEADVGIVFGPDAVQESQRVRIVAVS
                     ERGIIPPVTHSMAMERYCPDRALCAEFLSFIQSDEAQKELQRLGYTSPPQSQSMKKE"
     gene            2444..3094
                     /locus_tag="VFR82_00515"
     CDS             2444..3094
                     /locus_tag="VFR82_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500108.1"
                     /translation="MRFMSTDGLIFLLFVSIMSGGCARLPYTTKVIHEDRRVVISLQR
                     DPGETVPYTHPVQLKGDELSAVLAGFSFREKQRLPLRWFAEEVPPKKIFRSDEMQAVV
                     PFLVEGLERAAPDERVYFQVVAPGMNPAAERDTTAGWIVVREPYFYLVLEHYHAQFPI
                     RKSEQWDLRYPTTPPEPKTYLLYFEPGRFWETDPVSSRQAVQLREFLKTAIPASSR"
     gene            3161..4915
                     /locus_tag="VFR82_00520"
     CDS             3161..4915
                     /locus_tag="VFR82_00520"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEU4500109.1"
                     /translation="MESWSLLQNISQAIHRNKVVVLGALILFMLAPVSTHAVESGKLI
                     EKDGQYVFVESMDPATRLLLERAYDKGIITQEERDKAIKDSETRAYLMQPSFKLWYDR
                     GFNFSMNDNAFLLKIRGRLQLRETTRWRNDAWRNPGDAKNFPELLGVFGDYRANRSDD
                     FASQFNLRRARLLFLGHLLSPDFKYFLQIGFETAENAQTPGSANLLDYYVLSTHLPLL
                     NVQLGQYKVFFNRSQINNTASMQFAERAPVMDAFTASGMNRRDIGITIMNDDEIYPLN
                     YYVGIFNGAGPLFNRYGTYNSEEATVGCPGGQTGGNPFPSPAGCPANSRNLNANFRDE
                     IEKLMYAGRLQWNILGRPGYGEGDLVYSERPQMAVGGGLAYNPGINTSTDNAFVGIDL
                     ANLNFRRQLATFGNGRQLGWGVINYTTYGFDSVFKYRGFSLQGEWYFKNMERTFKGAP
                     CLQFNAAGACTAVTPQLLGNATGWYVQSGYYLIPRKLEVAARYSYWDPDTNASGDLIK
                     QVDASLNWFPFGTYDYQLMLTYSNMAMGTGGYAIGRSAPLPTQPPGAVNPSGLVPLDA
                     VGGTLIENAIRVQLQIFF"
     gene            5092..5889
                     /gene="modA"
                     /locus_tag="VFR82_00525"
     CDS             5092..5889
                     /gene="modA"
                     /locus_tag="VFR82_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4500110.1"
                     /translation="MIVPHWLQRVTYTALISFAVFICQFSVAGHAFSEEITVAAASDL
                     NYAIKEVIEGYQTATGNHVKLSLGSSGNFYAQIQNGAPFDLYFSADIAYPKKLEEAGL
                     IVPGSLYRYAIGRIVLWTSHTANRDVSRGLDVLRDPGIKKIAIANPKHAPYGRAAVAA
                     MQHFKVYDDAKERLVLGENISQAAQFIESGACDVGVIALSLALAPTMKAAGTYWEIPS
                     DAHPPLEQGAAILKSSRQQKTAQEFVDFMRGTHGQEIMSRYGFTLPK"
     gene            5867..6184
                     /locus_tag="VFR82_00530"
     CDS             5867..6184
                     /locus_tag="VFR82_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500111.1"
                     /translation="MDLHFRNRPVWLLTLILSSGCSDAVQMTHETEQGGVVTFLFKED
                     RGGPMGSPHRKEALKLIDQKCPAGYVVVRDGELKGYGSMSSVEGQEGDVTNRRWGIQF
                     RCK"
     gene            6222..6425
                     /locus_tag="VFR82_00535"
     CDS             6222..6425
                     /locus_tag="VFR82_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEU4500112.1"
                     /translation="MKLSARNQFQGTVTRITEGQAMAEVSVKVGNLEFIAAITEGSVK
                     NMGLKVNDSVTVAVKATEVMIGK"
     gene            6569..7243
                     /gene="modB"
                     /locus_tag="VFR82_00540"
     CDS             6569..7243
                     /gene="modB"
                     /locus_tag="VFR82_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEU4500113.1"
                     /translation="MNWTAIWVTCKLASLTSMALLVIGLPIAYWLTFSKWRWKFLIES
                     IVALPLVLPPTVLGFYILVAIGPHSPLGRLYNDVVGETLPFSFQGLLVASILYSLPFA
                     VQPFATAFEQVDRKLIEASWTLGLSKLKTFFKLIVPLSIAGLITGAVLSFAHTLGEFG
                     VVLMVGGNIEGVTRTVSIDIYDDVQALNYAAAAKTALFLLAVSYVVLLFIYAMNRKVW
                     AVWPQK"
     gene            7228..8319
                     /locus_tag="VFR82_00545"
     CDS             7228..8319
                     /locus_tag="VFR82_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4500114.1"
                     /translation="MAAEIAIDITKTFPGRPAISAKLAYTLQQSTALVLFGPSGSGKT
                     TILRCLAGLEWPERGTIRFVSRTWLDTGSRIRVSPQERLIGYMAQDYALFPTYTVAGN
                     ISYGLDALSTHERKKRVAEMLELFQLTGLEEAKPDELSGGQQQRVALARAIAPRPQLL
                     LLDEPLSALDAPTRVQLRGELRSLLKHLALPSVIVTHDWAETLTLGDVVAVVGDGHVL
                     QVGPPQEVFSRPVNAAVAQIVGVETVIEGDVIEQANGLATVRVNGTTLKGLGTNQVGS
                     AVFVCIRAEDVLLESAGSGMTSARNHLQGKVSEIIPHGVMVHVKIDCGFTVIAVVTKG
                     AQEELRLEQGSPVVAMIKAGAVHLVPRSM"
     gene            complement(8338..9033)
                     /gene="traT"
                     /locus_tag="VFR82_00550"
     CDS             complement(8338..9033)
                     /gene="traT"
                     /locus_tag="VFR82_00550"
                     /inference="COORDINATES: protein motif:HMM:NF017619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HEU4500115.1"
                     /translation="MLTLSMGCSNVIRAGLMNSNTIFLDPNTNRTIYLQLRNTSENQQ
                     ITLSEVETKLAAKGYQLMKDPVQANYWVQAKIIYCHQAADDVTPESVATAGFGAGISS
                     GGRTMGAASSNGNGMAGLFGSNMPTGNMAGMDMNAMMRMAMAGGGQGGFPGMQPPPKE
                     EGVTYLCVADVQITDRKIGKPIGQPLAGKEAAVLKAQQMRSVGHVRQKDLDIPEATPI
                     IADKLSTGITGLF"
     gene            complement(9072..9782)
                     /gene="traT"
                     /locus_tag="VFR82_00555"
     CDS             complement(9072..9782)
                     /gene="traT"
                     /locus_tag="VFR82_00555"
                     /inference="COORDINATES: protein motif:HMM:NF017619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HEU4500116.1"
                     /translation="MGYVLVLFLIAVPLTAQAGNIKETDLIASNVFFLPPSTAKTVFI
                     QTRNSSDNQGVSLHDLGARLSAKGYEIVQDSSAAHYIVLANIVYCNVTKPEMPVETMV
                     ASGYGSGFGSSMMSGLNSLTGMASMAGPQGALAGAAASMGLGAIEGIGSAVGSLFSGP
                     SKPKMPDDVNYACVADLQITEQVAAVPVLTSTNDQAAPAGVYQMRLAADVHQKKMNEA
                     EATPLLQQKLSAAVAGNF"
     gene            10356..10901
                     /gene="lexA"
                     /locus_tag="VFR82_00560"
     CDS             10356..10901
                     /gene="lexA"
                     /locus_tag="VFR82_00560"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEU4500117.1"
                     /translation="MKPTDLRRIREQLGLTQQQLADALHTTRVSVARYEAGMRRIPGM
                     VKVVIDRLGRAPEIPMAGMVAAGFPIEPVQQSELIDVPPSMLQGGKTFALRVKGESMK
                     DDGILPGDLIVVRKQETAQSGQTVVALVNGEATVKTYFKKGTHVELRPANTTMPPILV
                     QPSDEFHIEGVLIGVIRHCAI"
     gene            11135..11317
                     /locus_tag="VFR82_00565"
     CDS             11135..11317
                     /locus_tag="VFR82_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500118.1"
                     /translation="MLVQEPDIDVKRSGPCADCGIRVERRIECVDGLTHSTVELCQAC
                     WIACQQRQAFAGGCCG"
     gene            11329..12123
                     /locus_tag="VFR82_00570"
     CDS             11329..12123
                     /locus_tag="VFR82_00570"
                     /inference="COORDINATES: protein motif:HMM:NF020014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500119.1"
                     /translation="MQAVIHTAEATQLPFVEAESQNELLGRRGKTREWFLALRHAQIP
                     FHSDDRLIMLYGHALVFRLGLHAATERVLAGEPVVYLDGANTFDPFVVGQLARAHRRQ
                     PRAVLSMIHVARAYTSHQMERLISDCLMFALERYQSRIAILSGLFDTHYDQAVPEHDE
                     MRFFGRIMEASQRLTEQGHVLFFLCPQLSVMTHMAQRWFDRLRLHADRVIRVSEERGL
                     VKLQEEGEAVGQSWEIARTALERDQAGDGHHSRARSQRTHQGAPLR"
     gene            complement(12189..12365)
                     /locus_tag="VFR82_00575"
     CDS             complement(12189..12365)
                     /locus_tag="VFR82_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500120.1"
                     /translation="MNDGLRDDFVAGMDEQRFENVSVKRITEIEALWRINAESNTLLS
                     QEPDARAGIWKLAA"
     gene            12606..13283
                     /locus_tag="VFR82_00580"
     CDS             12606..13283
                     /locus_tag="VFR82_00580"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HEU4500121.1"
                     /translation="MGFVHTASRPFLFKECSELREILGEEAYDEKRLVELLETVPLDS
                     IYFHTHSYFLRHSYVERVYPNDFAQWVAMEVRDHVLGERLAVVDPFEYGELDALRGQL
                     ISIIDDHLSRTPIVPRVMFGEPFYFNKSLIIQVPTRIEVRTLQEFRSALSEVEVSAIY
                     FHVFEARHRLKHEESDFSAWIQDGLGMPDLAEKLRAINPYLGSLERVRSALLTVCDEF
                     LNREPKP"
     gene            13304..14977
                     /gene="treS"
                     /locus_tag="VFR82_00585"
     CDS             13304..14977
                     /gene="treS"
                     /locus_tag="VFR82_00585"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEU4500122.1"
                     /translation="MPDPLWFKDAVFYELHVKAFHDGNDDGIGDFRGLLEKLDYIQWL
                     GVDCLWLLPFFPSPLRDDGYDVANYRAITPEYGSLEDFRNFLDAAHQRGIRVIVDLVL
                     NHTSDQHPWFQQARSSPESATRDYYVWSQTDKRYEKARIIFIDTEKSNWTWDPAANAY
                     YWHRFFSHQPDLNYDNPEVQREMLDIMSFWLDEGLDGFRCDAVPYLFEREGTICENLP
                     ETHAYLKEIRRRLDQTYGDRILLAEANQWPADVRPYFGNGDEFHMAFHFPLMPRLFMG
                     VRSEDWHPIVDMFTHTPPIPENCQWCLFLRNHDELTLEMCAGEERDYMYYAYARDPQM
                     RRNIGIARRLAPLLDNDRRKIELLNSLVFTLPGSPIIYYGDEIGMGDNVHLGDRNGVR
                     TPMQWNADRNGGFSKADPLKLYLPPISDSVYGFHVTNVDSQRQSPHSLLHTMKQMIAR
                     RKTQPAFGRGDLTFLRPKNDKVLAYIRRYRGNELLLVHNLAGSAQPVELDLSAFQGQV
                     PIELLGGSRFPRISKGLYSLSLAPYGFYWFSLEQPQRDDPSYGIEGSVV"
     gene            15001..16200
                     /locus_tag="VFR82_00590"
     CDS             15001..16200
                     /locus_tag="VFR82_00590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4500123.1"
                     /translation="MAHELDEYRDHAPKGTVEFLYRLSDQVAGHSFLHISAGRYGGGM
                     AEILRRLIPMLNTLGINARWEVLAGDQEFFQASRRLANSLQGQDDYLTDQMQEVYLKI
                     NARTAQSLNLDADLVMIHDPPPAALIEHRKGGKWFWRCYLDIAKPRQHSWSFLRRFLV
                     GYDAAIFSLPGFAHRLPIPQFLISPSIDPLSEKNRPMARVEVNQVLSRLGIPRDKPIL
                     LQLARFEWFKDPLGAIRAYRLVRKHHNCRLVLAGSGVMHDPEGEAVLAQVREAAAGDP
                     DIHILQLPPEADVEINALQRAATIILQKSFKEGFSLAVTEAMWKGKPVIGSTAGGLAA
                     QILDGVTGYTVHSVEGAAFRARHLLNNPGLLQRMGGAGREHVRRNFLVTRHLSDYLTL
                     MRLFVAR"
     gene            16244..17524
                     /locus_tag="VFR82_00595"
     CDS             16244..17524
                     /locus_tag="VFR82_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU4500124.1"
                     /translation="MGEAVWEQVQEQCSDVRKVDLLVALPSFNQASTVVPAVRTIVAG
                     LKSFFGHASILFVNADVGSQDGTPDLVRQTVGDLLPFASIRPNTESMSGSTFALNRFS
                     YSGMPAREEAFRSVFAVTRRLEARACIVLDSSVRSVTAEWVSLLAQPILEKGVDYVAP
                     VFNRQRYEGSLTNNLIAPLTRALYGKRVACQIGGGYGFSQKLSSFLLENEALWEGDAG
                     RFAIDSWVTTVAIAEGYQVWQACLGAKVQDIKAGAADISTSLAQAVGATFHYMERYQD
                     IWERQVGSEEVPVVGRPIEIGMESVPIQVDRMLKGFRQGLRDLLPIWEVILAPDTLAG
                     VLELGLRDDEEFRFPLPLWVQTVYDFAIAYHEKVIHREHLLKSLVPLYLGRTASVVLE
                     TKGGGPGLVQDTVEHGCQTFEVTKPYLIDRWRFQ"
     gene            17521..18219
                     /locus_tag="VFR82_00600"
     CDS             17521..18219
                     /locus_tag="VFR82_00600"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500125.1"
                     /translation="MSEMWQDAMVAAFREMASRGVLFLPKLMALVTFLMLGLAIGWLV
                     KVSLMRILNVVRFEVFCERMGLSPALTKAGMKQQIPHLVGRLAFWIVFLFFAFMGVDA
                     LDLPATANLMSAILGFLPNVIAAILVALLGILLANFTGEAVLIAAVNAQIHGARFIAN
                     MVRWGVVIFTVAMVLTQLGIAKEIVVAAFSIIFGGIVLAMAIALGLGGRSIAKEVLER
                     RFGREKSRDEFTHI"
     gene            18245..20239
                     /locus_tag="VFR82_00605"
     CDS             18245..20239
                     /locus_tag="VFR82_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEU4500126.1"
                     /translation="MTHQDGRQRVAIEGVRPEIDGGRFPIKRTVGEEIVVEADAFADG
                     HDVVVVILRYRLRDSTEWTDLPMKHVGNDRWRAAFHVSTMGQWHYTITGWVDHFETWR
                     RDLVKKLDAGQNLQVDLLIGAKLIDSAVARASADDGRKLTAWRQALRSNESPDDDRIR
                     IALSEDLRALMARYPDRRLASVYERELRVIVDRQKARYSTWYELFPRSWSRTPGQHGT
                     FKDCEAALPYVASMGFDVLYLPPIHPIGQTHRKGKNNAPTGGAADPGSPWAIGALEGG
                     HKSIHPELGTLQDFRRLREQASTYGIEIAMDVAFQCSPDHPYVKEHREWFRIRPDGTV
                     QYAENPPKKYQDIFPLEFETDQWQALWDELKSVILYWSNEGVRVFRIDNPHTKPFPFW
                     EWVISETKQIYPDVIFLAEAFARPKVMLRLAKLGFTQSYNYFPWRTTKSELTQYFTEL
                     TQTDIREYFRPNLWPNTPDILTEQLQHGGRAAFMSRFVLAATLGASYGIYGPAFELGE
                     CVPREVGSEEYLHSEKYEIKQWDLRREDSLSEFIGHVNRIRRENPALQSDWSLHFHEV
                     GNDQLLCYSKRTSDGANVILVVVNLSPYHVHSGWLELDLAHLGVELDRPFQVQDLLTN
                     AHYLWQGRRNYIEINPHSAPAHIFRVKGKVHSERDFDYYF"
     gene            20128..21891
                     /locus_tag="VFR82_00610"
     CDS             20128..21891
                     /locus_tag="VFR82_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HEU4500127.1"
                     /translation="MAGTAQLYRDQSPFRSGSYFPRQGEGTQRAGFRLLLLTEAGLPD
                     CETMLTAKHDWARLLEGDAAADLMRVLCEILQTRRWFGGKARRIAEVRIVDSIAVPSD
                     SSSILLLLCVDYRDGESEIYSLPVTAVFGEDAERVQREIPRAVIAPFMLTKNGTTDIG
                     ILYDALWNREFTAELLAALRNESRFQGAVGAVTASVTAAFKDLVPSGIRLDPRVMGSE
                     QSNTSVVYDGHVILKLYRRLEEGINPDLEIGRVLTRMQFPFVPALAGAIEYQRPSREV
                     STLGVLQRYVDNAGDAWTWSLQTVKEFFTRVGSLSLDRHATVDKSSLLELAGEPYPTP
                     VRHFMEGYLESAERLGQRTAQLHLALSRVEDDPAFAPEPLTLEYRQARYEAALRGMTS
                     TLALVKARMDSLPTAAQEQANVLFRFRPTLERIFDAFRNLDTPGSLIRCHGDYHLGQV
                     LFTGADFVITDFEGEPARPLAERRMKHPPLVDIAGMVRSFQYVPFAFLKGAGLPPSPW
                     CPFWSNWACVAFLKGYLSAATGSELWPQTNGDVRPLLDAYIAEKAFYELQYELNNRPD
                     WVDIPLHGLAAILNIREESER"
     gene            21924..>22160
                     /locus_tag="VFR82_00615"
     CDS             21924..>22160
                     /locus_tag="VFR82_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500128.1"
                     /translation="MTRIQFTPRKPGKEMTLLTKDDLYLFNEGTHFHLYEKLGAHPLE
                     SEGTRGTHFAVWAPNAEHVSVIGTFNNWQRGAHVL"
BASE COUNT         4903 a         5791 c         6305 g         5161 t
ORIGIN      
        1 gcttttttca cgtaatgggg cactggcgag tccaggcgca tcttttctct gcgctcaata
       61 agaaccacct tcgaatcgtg gaccgtgatt tcaacggacc caaaatggag atctctcaac
      121 gcctgctgaa tggccagttc cacccggtaa ttttgttccg ttgtatgcat ggcgggcctc
      181 attccatgga ggatttgttt aattactagt aacccagtcg aattagtaat aatagcttat
      241 aattaatatg atcatatttg tcaacaaata actttatgca gtgccatatg cagtcacata
      301 attggttgtg ttggaaaata gctttacaac gttagcaagt atacttgact taaggcaaat
      361 ataaatgcta ccaccgcagt catggcagaa ccgtcatcta tccgttttca caatcgactg
      421 aaggatgtcc gtgttgcaag ccggttgtcg caaggcgatc tagcttcgaa agctggaatc
      481 acacgtcagg cagtttccgc aatcgaatcc gaactctatc ttccaaccac agccgtggcc
      541 ttacgactgg cggctgttct ttcttgtcgc gtcgaagatc tattcagtct tgccgcctct
      601 gaggaagtgg tcgagggcag atacatcggc ggacaggaca agtcaccgac tgtccctctg
      661 cgtgtgaaag ttacgaaagt gggaaagaac atcatcgttc gccccgtcgc cgagttgggc
      721 gaagtgttgg cctacaccgt gccggctgat ggatatttac ttgagacgac aactgccaaa
      781 tcgggggcca cggcgcgtgt ccggctttct cgggataggg aagcgattga tgaggaaatc
      841 tcggtcgccg gctgcgatcc agccattttt cttgctggag cacacctgcg ccgccataag
      901 gatcgaactt cagttatcgg gtggtcgatg ggaagcatgg cagccgtcgg agccttgcag
      961 cggagtgagg tgcatgtggc cggcctgcac ttgttcgacc cgctcagtgg cgaatcgaat
     1021 ctgccgtttc tcaagcgcgc gctcaaaggg tcggactacg aggtggtgac gtttgcaacc
     1081 tgggaggaag gctttctggt caggcccggg aacccaaagg cgatccgcca ggtggccgat
     1141 ttggccgacg agacggtgat attcgtcaat cgagaggagg gctctggtgc acggctcttg
     1201 ctcgatcagc ggttgcgggc tgcaggtgtg gatcatgcca gggtgcgtgg atatgagaac
     1261 actgcccgct cccattttga cgtcgcgaga acgatagcag gcagacaggc cgatgtcggt
     1321 atcggcattc gatcggccgc tcaatccttc ggcctgggtt tcgtgccgct tcaatcggcg
     1381 cgctacgacc tcgtcgtagc taaatccatt ctccagagtc atcccagtct cagcaattta
     1441 tttgacgcca ttagcagccg gcggtttcgc gaagagatcg aagcgttagg cgggtacgac
     1501 acacgagaaa cgggaaagct ccatgcgctg cgatcgtgaa cgacttcacc gaagaggaag
     1561 agtgtcatga gaccgtgcgt gctgctctat ggtctgtttc tcatagtgtg gatcatgacg
     1621 gcctctgcct atgcgaccga gtctcttatt atcgcgacct cgccaagtgt ggcgccggct
     1681 gtagaagcat tgggacgcca atttgaaaca actcatccgg acgttcgcgt gcgtctgtac
     1741 ctcgattccg ggctcaacct tcgtcgaacc atcgctggaa tggaaaacaa tccgagaggt
     1801 caatacttca tcggttctgg gccaatccat ctcgttgcac ccggtggaga cgaacttctt
     1861 actcgcttgg agcaaaaata ttatgtcctt ccaggtacga ggcgacccta tgcggaagtg
     1921 ccgttagtca tggtcgtgcc cgaggcgctc gttgatgcgc cgacgtcgtt tgaatcactc
     1981 acacaaaaca aaaccttgcg gattgccgtc ggtgatccag cgctgaccat gctcgggcaa
     2041 aggacgatgc aactgctgac gacgttaggc atctgggccg cggtagaaaa ccgattggat
     2101 cgagcaagcg acgtccgttc ggtgttggat catgtcctca atggtgaggc ggatgtcgga
     2161 attgtctttg gtccggacgc ggttcaggag agccaacgcg ttcgtatcgt ggccgtttcc
     2221 gagcgcggca tcattcctcc agtgacccat tcaatggcca tggaacggta ttgtcccgat
     2281 cgagcactct gcgcagagtt tcttagtttc attcaatctg atgaagccca gaaggaactc
     2341 cagcgcctgg ggtacacatc gccgccacaa agccagtcca tgaagaaaga atgaatacca
     2401 atacgcagtt atttgagagg agggggcggt gatgtggagt cggatgaggt tcatgtcaac
     2461 ggatggtctt atatttttgt tattcgtgag catcatgtcg ggagggtgcg cccgacttcc
     2521 gtacaccacc aaggtcattc acgaagatcg tcgtgtcgtc atttcacttc aacgggatcc
     2581 aggtgaaacg gttccttaca cacatcccgt ccagttgaaa ggggatgaac tttccgccgt
     2641 gctcgcgggg ttctcgtttc gcgaaaagca gcgattgccg ctccgatggt tcgcggagga
     2701 agtgccaccg aagaaaattt tccgttcgga tgaaatgcaa gccgtcgtgc ctttcttggt
     2761 cgaaggtctc gaaagggcgg ctccagacga gcgagtgtac tttcaagtgg tcgctcccgg
     2821 catgaatccc gccgctgaac gcgacacaac ggcgggatgg atcgtcgtga gagaacccta
     2881 cttttatctt gtgctagagc actatcacgc ccaatttccg atcaggaaat cggaacagtg
     2941 ggacctgcgt taccccacca ctcctccgga gccaaagacg tacttgctgt acttcgaacc
     3001 ggggcgcttc tgggaaacgg atccggtgag tagtcggcaa gccgtacaac tgcgcgaatt
     3061 cttgaagacg gccattcccg cctcttcaag atagtcactg aacataggtc ggcgtcatct
     3121 gaaattatca ttatttcttt cacttctcgt ggagggagtc atggaatcct ggagccttct
     3181 tcagaacatc tcacaggcaa tacaccgaaa taaggtcgtt gttctgggtg cgctcattct
     3241 gtttatgttg gcgccggttt caacacatgc cgtggaatca ggcaagctga tagaaaaaga
     3301 tgggcaatat gtgttcgttg aaagcatgga tcccgcaacc cggctgttgt tggaacgggc
     3361 ctatgataaa ggcatcatca cacaagagga gcgagacaaa gcgatcaagg actccgaaac
     3421 tcgcgcctat ctcatgcagc cgagtttcaa actttggtac gaccgaggct tcaacttttc
     3481 gatgaacgac aacgcgtttc tcctcaagat ccgaggacgc ctgcagttgc gtgaaaccac
     3541 acgatggagg aacgatgcct ggagaaaccc cggcgacgcc aaaaatttcc ccgaactctt
     3601 gggcgtgttc ggagactacc gagcgaaccg atcggatgat tttgcctcgc agttcaattt
     3661 acgacgggct aggctcttgt ttcttgggca cctcctcagt ccggacttta agtattttct
     3721 tcagatcggg tttgaaacag ctgaaaatgc acagactcct ggatcagcca accttctaga
     3781 ttattatgtt ctcagcaccc acctcccgct cttgaacgta caactcggtc aatacaaagt
     3841 ctttttcaat cgatcccaaa tcaataacac ggcctccatg cagtttgcgg aacgggcccc
     3901 tgtaatggat gccttcacag cgagcgggat gaatcgacgt gatatcggca tcacgatcat
     3961 gaatgacgac gaaatctacc ccctcaatta ttatgtcggg attttcaacg gcgctgggcc
     4021 gctctttaat cgctatggaa cctacaacag tgaagaggcc acggtgggat gtccgggtgg
     4081 gcaaacgggc ggcaatccct ttccttcccc cgccggttgt ccggccaatt cgcgcaatct
     4141 caatgcgaat tttcgcgatg aaatcgaaaa gctcatgtat gcgggacgac tccaatggaa
     4201 cattctcggg cgacctggat atggtgaagg agatttggtt tattcagaaa gaccgcaaat
     4261 ggctgtcggt gggggattgg cctataaccc tggcatcaac accagcacag acaatgcgtt
     4321 cgtcggcatt gatttggcga acttgaattt ccggcgacaa ttggcaacgt tcggcaatgg
     4381 gcgtcagttg gggtggggag tcatcaacta cacgacttac gggttcgata gcgtcttcaa
     4441 gtacagaggc ttttcgctcc aaggggaatg gtacttcaag aacatggagc gaacattcaa
     4501 aggtgcgcca tgtttgcaat tcaatgcagc cggcgcctgt actgccgtga cgcctcagct
     4561 tttgggcaac gctacggggt ggtatgtcca gtcggggtat tacctgattc cacgcaagct
     4621 tgaggtggct gcgcggtact cttattggga tcccgatacc aacgcatcag gcgatttgat
     4681 caaacaggtg gatgcatctc tcaattggtt ccctttcggg acttacgatt accagctcat
     4741 gttgacgtac agcaacatgg cgatgggtac aggcgggtat gcgatcggcc gcagtgcgcc
     4801 gctgccgaca caacctcccg gtgccgtcaa cccttcaggc ttggttccat tggacgccgt
     4861 gggtggaacg ctgatcgaga acgcgatccg tgtgcagttg cagattttct tttaagatgg
     4921 agcggccatt agccgcgcag ggcattactg gtcgggagca ttggccattt tcatgcggat
     4981 ggtgtcatta tgaattcggt cagcggtcgt ctgtccagta tgcggggaca ctacatgtat
     5041 cgtttgcgac aacgtgttgt ttcgtcaatt cttaagggaa ggatgttcct tatgatcgtc
     5101 cctcactggt tgcagcgcgt cacgtataca gcgttgattt cgttcgccgt cttcatctgc
     5161 cagttcagtg tcgccggcca cgccttcagt gaagaaatta cggttgcggc cgcctccgac
     5221 ttgaactatg cgatcaaaga ggtgattgaa ggatatcaaa cggcgaccgg caatcatgtg
     5281 aaactgtcgt taggatcgtc cgggaatttt tatgcacaga ttcaaaacgg tgcgccgttc
     5341 gatttgtatt tttctgctga tatcgcttac ccgaagaagc tcgaagaagc cggtttgatc
     5401 gtgcctggat cgttgtatcg gtacgctata ggccggattg tcctttggac cagtcacaca
     5461 gccaatcgcg atgtctccag ggggctggac gttctccgtg atcctggcat caagaaaatc
     5521 gccatcgcca atccgaaaca tgccccctac gggcgagccg ccgtggcggc gatgcagcat
     5581 ttcaaggtct atgacgatgc caaggaacga ttggtcttgg gcgagaacat ctcgcaagcc
     5641 gcccaattca ttgaatccgg cgcctgcgat gtcggcgtta ttgcactctc gcttgccttg
     5701 gcgcccacca tgaaggccgc aggcacctat tgggaaattc ccagtgatgc ccatccccct
     5761 ttggaacaag gagccgccat cttgaagtct tcgagacaac agaagacagc ccaagagttc
     5821 gtggacttca tgcgaggcac gcatggacag gagatcatga gccgctatgg atttacactt
     5881 ccgaaataga ccggtctggc tgttgacgct catcctctcg agcgggtgtt ccgatgcggt
     5941 gcaaatgacg catgaaacgg agcagggcgg tgtggtcacc ttccttttca aggaagaccg
     6001 gggcggtccg atgggatcgc cgcatcgaaa ggaggcattg aagctcatcg accagaaatg
     6061 cccggccgga tacgtggtgg tgcgggatgg agagttgaaa ggctacggca gcatgtcgag
     6121 tgttgagggc caggaaggag atgtcacgaa ccgccggtgg ggaattcagt ttcgatgcaa
     6181 ataattcacc gaacatgcga atcaaaccat aggaggacaa catgaaactt agcgcacgca
     6241 atcagttcca gggaaccgtc actcgcatta cagagggaca agcgatggcg gaagtgtcag
     6301 tgaaggtcgg aaatcttgaa ttcattgcgg cgatcacgga aggctcggtg aaaaacatgg
     6361 gcctgaaagt gaacgactca gtcacggttg cggtgaaagc cactgaagtg atgatcggca
     6421 aataggggat gtcaggacgg tcaaaaaggt catcagccag gccgcaacaa gcgcagagat
     6481 gagccgtgct ttcgctcgta cgttgagcct ctaagcgatg cgagaacaaa gctgacggcc
     6541 tttttcatca tcctggaaag gcagcattgt gaattggacc gctatctggg tgacctgcaa
     6601 actggcgagc ctcacttcga tggcattgct cgtgatcggg ttaccgatcg cctattggtt
     6661 gacgttttcg aagtggcgat ggaaattcct catcgaatcg atcgtggcgc ttccactggt
     6721 tctcccgccg acagtcctgg ggttctatat tctcgtcgcc attggaccac acagcccctt
     6781 gggccgatta tacaacgatg tcgtcggtga gaccttaccg ttcagctttc aaggcttgct
     6841 agtcgcctca attttgtaca gcctgccttt tgccgtccaa ccgtttgcca ctgcctttga
     6901 gcaggttgat cgaaagctga ttgaggcttc atggaccttg ggcctttcca aactaaaaac
     6961 ctttttcaag ttgatcgtgc cactttcaat agcggggctc atcacgggtg cagtcttgag
     7021 ctttgcccac acgctcggtg agttcggggt tgtcttgatg gtcggaggaa atatcgaagg
     7081 cgtcacccgg acggtttcta tcgacattta cgacgacgta caggcactca actatgccgc
     7141 agcggcaaag acggcgctgt ttctcctcgc agtgtcctat gttgtcttgt tgtttattta
     7201 tgcgatgaat cgaaaagttt gggcagtatg gccgcagaaa tagccatcga catcaccaag
     7261 acgtttcccg gccgtcccgc aatcagcgca aaactggcat atacactgca gcagagtacg
     7321 gcattggtgc ttttcggtcc ttcgggatct ggcaagacca ccatccttcg atgtcttgca
     7381 ggactagaat ggcctgaacg gggaaccatc cgatttgtgt cgagaacctg gcttgatacc
     7441 ggttccagga tccgggtttc gccacaggag cggttgatcg ggtatatggc gcaggattat
     7501 gccttgtttc cgacttatac cgtggcgggt aatatctcgt acggcttgga tgcgctttcg
     7561 acacacgagc gaaagaagcg cgtagccgag atgcttgagc tcttccaact caccggcctt
     7621 gaagaggcca agccagacga attgtccggg ggacagcagc aacgcgtcgc tctggccaga
     7681 gcaatcgcac cccggccgca gttgcttctc ttggatgagc cgctttccgc actcgatgct
     7741 cccacgcgcg tgcaactcag aggagagctg cggagtctgc tcaagcactt ggctttgccc
     7801 tcggtgattg tcacccatga ttgggctgag acgttgactc tcggggatgt ggtagccgtg
     7861 gtgggcgatg gacacgtact acaggtcggt cctccacaag aggtcttttc tcgaccggtc
     7921 aatgcagcag tggcgcagat cgttggagta gaaaccgtta ttgaaggaga cgtcatcgag
     7981 caagccaatg gtttggccac cgtgcgcgtc aatggtacaa ccctcaaagg gttggggacg
     8041 aatcaagtcg gctcggctgt gtttgtttgt attcgcgcgg aagatgtgct gcttgagtcg
     8101 gcagggagcg ggatgaccag cgcccgcaat catttgcagg ggaaggtgag cgaaattatt
     8161 ccacatggcg tcatggtcca tgtgaagatc gactgtgggt tcaccgtcat cgccgtggtg
     8221 acgaaaggag cgcaggaaga gctacggctc gagcagggtt caccagtggt cgccatgatc
     8281 aaagcgggcg ccgtgcatct agtgccgcgc tcaatgtaac gatgtgcata ctgaggctta
     8341 aaacagaccg gtgatgccgg tacttagttt gtcagcgata atcggagtgg cttccggaat
     8401 gtccaagtct ttttgccgca catgcccgac cgatcgcatc tgctgtgctt taagaacagc
     8461 agcctccttg cctgccagcg gttggccgat cggtttaccg atcttgcgat cagtaatctg
     8521 gacatcggcg acgcagagat aagtcactcc ctcttctttc ggaggaggtt gcatgccggg
     8581 gaagccgccc tgcccgcctc cggccatggc catgcgcatc atggcattca tatccatccc
     8641 tgccatatta cccgtcggca tgttgcttcc aaacagaccg gccattccat tgccgttgct
     8701 gctggcagcg cccatcgtcc tcccaccgct gctgatgccg gcgccgaatc cggccgtggc
     8761 aacggattct ggagtcacgt cgtccgcggc ttggtgacag taaataatct tggcttggac
     8821 ccagtaattg gcttgtacag gatctttcat caactgatat cccttggcgg cgagtttcgt
     8881 ctcgacctcg ctgagtgtga tctgttggtt ctcagaggtg ttgcgcaatt gaaggtagat
     8941 ggtgcgattg gtattgggat ccagaaagat ggtgttgctg ttcatcagac cggcgcggat
     9001 gacgtttgag caacccatgc tcaacgtcag caacagccca actcctagga agaggaaagt
     9061 acatctcatt atcagaagtt tcccgcgaca gcagcgctta gtttctgctg caataaaggg
     9121 gtggcttctg cttcgttcat cttcttctga tgcacatcgg cagcgagacg catctggtac
     9181 acaccagccg gtgcggcttg gtcgttggtc gaggtgagca ccggaacggc agccacttgc
     9241 tccgtgattt gcagatcggc gacgcaggca taattgacgt cgtccggcat tttcggtttc
     9301 gaaggtccgc tgaacaggct gcccaccgcg cttccgatgc cttcgatggc gccgagaccc
     9361 attgaggcag cggcgccagc cagggctccc tgcggtcctg ccatactggc catgccggtc
     9421 aaactgttca gcccactcat cattgagctt ccgaatccag acccgtagcc gctggcgacc
     9481 atcgtctcga ccggcatctc gggctttgtc acgttgcagt acacgatgtt cgccagaaca
     9541 atatagtggg cggcagagga gtcctgcaca atctcatatc cttttgccga cagtctggct
     9601 cccagatcgt gcaacgatac gccctggtta tcggaggaat ttctcgtctg aataaaaact
     9661 gtcttggccg ttgaaggagg aaggaaaaat acattactcg caataagatc ggtttctttg
     9721 atgttgccgg cctgagctgt gagcggaacg gcaatgagga atagcaccaa aacgtagccc
     9781 atcaattcct cgcggcaaag gggtacgaat tctacgcagg gcaaacccag aatatcaacc
     9841 aacctatcgg aatcggagac tcaggttaga tgcacgaggg aactgagaat ccgtgaggat
     9901 ttggcaaaaa gcgcatgctt ggggtaaccg caccatgaca ttggtcggct gtaggggccg
     9961 ccgtaatttg cagcattcta aaggttgaac gcctttttcc gacgggacct aggggtctcc
    10021 ccaggttctg tggataaccc tgtgtgtaat gcaattccct ctgtggatct cttgcagtgg
    10081 aacggacaag cacgctttgc ctatattttt agcatagggg cgcgacacat gtgtgcccca
    10141 tatgtggtgg ttctacatca agaaattgaa catccgtatt ccctcatcgg acgcggaatt
    10201 tggctgttcg aaatgcggac ggcaagaccc agactgcgtt ttgtaaaaac ttatgcacag
    10261 acgccgcatt gacagcctcg tttcttactc tttttagtaa tcgcgatgag aggacttgac
    10321 agcgaggcgc cgcctgtgta accattggtt acagtatgaa gccaactgat ctcagacgca
    10381 ttcgtgagca actgggtctg acccagcagc aactagccga cgcgctacac acaacccgtg
    10441 tctctgtcgc ccgttatgaa gccgggatgc gtcggatccc ggggatggtg aaagtggtga
    10501 tcgatcgtct tgggcgtgcg ccagaaattc cgatggccgg catggtggcg gccggcttcc
    10561 cgattgaacc ggtgcaacaa tcggaactca tcgacgttcc tcccagtatg ttgcaggggg
    10621 gaaaaacctt tgcgctacgc gtcaaaggag aatcgatgaa ggatgacggc attcttccgg
    10681 gtgacctcat tgttgtgcgg aagcaagaga cggctcagtc gggacaaacc gtcgtggcgc
    10741 tcgtcaatgg cgaggccact gttaaaacgt atttcaagaa gggcacgcat gttgaattgc
    10801 gccctgcgaa taccaccatg ccaccgatcc tcgtccagcc gtctgacgag tttcacatcg
    10861 aaggcgtact tatcggggtc atccgtcatt gtgcgattta acaaggaggt gggatcgaac
    10921 atgatagtca cgagagaccg atttataact ctgcagcgga ccgtcgatac gttggaagga
    10981 cgactccagg acatccaatg cgagctacgg caggccaaag gcgtctcagt gaccgaacgg
    11041 gtttggaaaa atttactagc accctggcag gggagcgcgt ttatgtcttc ctgttgtaac
    11101 cggcgagtgc gacccaacac aaaggtggtg aatcatgctg gtacaagaac cggacattga
    11161 cgtcaaaagg agcggccctt gtgccgactg cggcatcagg gtggagcggc gtatcgaatg
    11221 tgtcgatggg ttgacgcatt ccactgttga actgtgccag gcctgttgga tcgcttgcca
    11281 gcaacggcag gcgtttgccg gcgggtgttg cggatagcga ggcgcattat gcaagccgtc
    11341 atacacacag cagaagcaac acaattgcct tttgttgaag cagagtctca gaatgaactc
    11401 ctgggcagaa gaggcaagac tcgagaatgg tttctcgcgc tgcgtcatgc gcagattccc
    11461 tttcattcag atgatcggct gatcatgttg tatggtcatg cgctcgtgtt ccggttggga
    11521 ctccatgccg caaccgagcg ggtcttggcg ggtgaaccgg tcgtctatct cgatggggcg
    11581 aatacgttcg atccgtttgt tgttggacaa ctcgcccgtg cgcatcggcg gcagcctcgt
    11641 gcagtattaa gcatgatcca tgtggccagg gcgtatacaa gtcatcaaat ggagcggctg
    11701 atttccgatt gtctgatgtt tgccctcgaa cgctatcagt ctcgcattgc cattctgtca
    11761 ggtttgttcg atacgcatta tgatcaggcc gtaccggagc acgatgagat gcggtttttc
    11821 ggtcgaatca tggaggcgag tcagcgactg accgagcagg gccatgtctt atttttctta
    11881 tgtccgcaac tttctgttat gacccatatg gcccagcgtt ggtttgatcg tttacgcctg
    11941 cacgctgacc gggtcattcg ggtgagtgag gagcgcggac ttgtgaagct acaggaagag
    12001 ggagaggcgg tgggacaatc gtgggaaatc gcccgcacag cattggagag ggaccaggcc
    12061 ggtgacggtc atcattcccg tgcccggtct caacgaaccc atcaaggggc accccttcgc
    12121 tgacgaggtt ggttgccaac aatgaactcc gctggtcaag gagcaagagc tatggaaccg
    12181 ctgcctactt atgcggccag tttccagatg ccggcccgtg cgtcgggctc ttgtgacagc
    12241 agagtattac tttccgcatt gatgcgccag agcgcttcaa tttccgtgat ccttttcacc
    12301 gatacatttt caaaccgctg ctcatccatg cccgctacaa agtcatccct caacccatcg
    12361 ttcattgggt ctccttccat ctaacagttc agtaaggcat actacgcgcc tcgaaacgcg
    12421 gcaactggtg tttcccctgg gaaacacctt ttacattcgc cggaacaagg aaagcatccg
    12481 ccgtccttaa ctggggtaat ccctaggtac cgactagccc acatattgtg aacaatggtc
    12541 taattgtcgg aagtccatga tccgcttaag gcgacttcaa attcagccgc taaaaggcgc
    12601 agggggtggg tttcgtgcac acggccagtc gtccgttttt attcaaagaa tgtagtgagt
    12661 tgagagaaat tctcggggag gaagcctatg acgaaaaacg gctggtcgaa ttgctggaaa
    12721 ccgtcccctt ggactcgatc tattttcata cccatagtta ctttttgagg catagctatg
    12781 tggaacgggt ctacccgaat gatttcgcgc aatgggtcgc gatggaagtg agggatcatg
    12841 tgttgggcga gcgtcttgcg gtagtcgacc cattcgaata tggagagctg gatgccttac
    12901 gcggccagct gatctccatt attgatgacc acttgtccag gacgccgatt gttccccgcg
    12961 taatgtttgg cgagcccttt tacttcaaca agtcactcat tatccaagtt ccgacacgga
    13021 tagaagtcag gacactgcaa gaatttcgga gcgcactgag tgaagtcgaa gtgagtgcaa
    13081 tctatttcca cgtcttcgaa gcgaggcacc gtctcaaaca tgaagagagc gatttttccg
    13141 cgtggataca agacggtctc ggcatgcccg atcttgcaga gaaacttcgt gccatcaacc
    13201 cgtatttggg gagcctcgag cgggtgcgct cagcgctgct tacggtgtgc gatgagttct
    13261 tgaaccggga gcccaaaccc tgagcaaaaa gagaagctct acgatgccgg atcccttatg
    13321 gttcaaggac gcagtctttt acgagcttca cgtcaaagcg tttcacgacg gaaatgacga
    13381 tgggatcggg gactttcgcg ggctcctgga gaaactcgac tatattcaat ggctcggcgt
    13441 cgattgttta tggctgcttc ccttctttcc gtcacccttg cgcgacgatg gctatgacgt
    13501 cgcgaattac cgggcgatca cgccagagta tggctcgctc gaagactttc gtaatttcct
    13561 cgacgccgcg catcagcgcg gcatacgtgt cattgtggat cttgtgctga atcatacatc
    13621 cgaccaacat ccgtggttcc agcaagcgag aagctcaccc gagtcggcca cgcgagacta
    13681 ttatgtctgg agtcaaaccg acaaacgcta cgagaaagcc cggatcatct tcattgacac
    13741 ggaaaagtcg aattggacat gggatcctgc ggccaatgcc tattactggc accggttttt
    13801 tagccaccaa ccggatttga attatgacaa ccccgaagtc cagagagaga tgctcgatat
    13861 catgtcattc tggctggatg aggggctgga tggttttcgc tgcgacgccg tgccgtattt
    13921 attcgagcgc gaaggcacga tctgcgagaa tcttccggag acccatgcgt atttgaaaga
    13981 aatacgcagg cgcctcgatc agacatatgg cgaccgtatc cttctcgcgg aagcgaatca
    14041 atggcctgcc gacgtgcgtc cctatttcgg aaacggcgac gaattccaca tggcctttca
    14101 cttcccgctc atgccaagac tgttcatggg cgtgcggagc gaggattggc atcctatcgt
    14161 ggacatgttc acgcatacac cgccgatccc ggagaactgt caatggtgtt tgttcctgcg
    14221 caaccatgac gaactgacac tggaaatgtg tgccggcgaa gaacgtgatt acatgtacta
    14281 cgcctatgca cgcgatccac agatgcggcg caatatcggc atcgctcggc gcttggcacc
    14341 gttgctggac aacgaccggc gaaagattga attgctcaac agcctggtct tcaccctccc
    14401 gggcagtccc atcatttact acggagatga gatcggcatg ggggacaatg tgcacctcgg
    14461 cgatcgcaat ggcgttcgga ctcccatgca gtggaacgcg gatcgaaatg gggggttttc
    14521 aaaggctgat ccgctcaagc tgtatttgcc gcctatctcg gattccgtct acggcttcca
    14581 cgtaaccaac gtcgattctc agcgccaatc accacattcg ctcttgcata cgatgaagca
    14641 aatgattgcc aggcggaaaa ctcaaccggc gtttggacgc ggagatttga cgtttcttcg
    14701 tccgaaaaat gacaaggtgc tcgcctacat tcgcaggtat cgcggcaacg agctattgct
    14761 cgtccacaat ttggccggat ctgcgcaacc tgttgaactc gacttgtccg ccttccaagg
    14821 acaagtgccc attgaactgc tggggggatc ccgcttcccc aggattagca aaggtctgta
    14881 ctcgctgagc ctggcgcctt acggatttta ttggttcagc ctcgaacagc ctcagcgtga
    14941 cgatccgtca tacgggattg aaggcagcgt cgtctagcgg cgcagtgatt ggaagaagag
    15001 atggcacatg aattagacga gtatcgtgac cacgcaccga aaggcacggt cgagtttctc
    15061 tatcgcttga gcgatcaagt ggcaggacat agttttttgc atatcagtgc aggccgctat
    15121 ggcgggggta tggccgaaat ccttcgccgg ctgatcccga tgctcaacac gttaggcatc
    15181 aacgcacgct gggaagtgct ggcaggtgac caggaattct tccaggcatc caggcggctg
    15241 gccaattccc ttcaaggcca ggacgactat ctcaccgacc agatgcagga ggtctacctt
    15301 aagatcaatg cgcgtaccgc tcaaagcctc aatttggacg ccgatctggt gatgattcat
    15361 gatcctccgc cggcagcact gattgaacat cgcaagggag ggaaatggtt ttggcgatgt
    15421 tacctcgata tcgccaagcc ccggcagcat agctggagtt ttctcaggcg tttcttggtt
    15481 gggtacgatg cggcgatctt ttcattgccg gggtttgccc atcgcctgcc cattccgcag
    15541 tttttgattt ctccatcgat cgatccgttg agtgagaaga accggcccat ggcccgtgtc
    15601 gaagtcaatc aagtgctctc gcgtcttgga attcctcgcg ataaaccgat cttgctccag
    15661 ttggcacgct tcgagtggtt taaagatccg ctgggagcca ttcgggccta tcggctggtc
    15721 agaaagcatc acaattgccg cctcgtgtta gcgggctctg gtgtcatgca cgacccggag
    15781 ggtgaagcgg tcttggcgca agtgcgcgaa gcggctgccg gcgatcccga catccatatt
    15841 ctgcaattgc cgccggaagc cgacgttgaa atcaacgcgc tgcagcgggc ggcgacgatt
    15901 atcttgcaaa aatcgtttaa ggaagggttc agtcttgccg tgactgaggc catgtggaaa
    15961 ggaaagcccg tcatcgggag taccgccggc ggcctggccg cacaaatcct agacggcgtc
    16021 accggctaca ccgtgcattc tgtcgaaggc gcggcgttca gggctaggca cctcctcaat
    16081 aatcccggcc tcctgcaacg catgggcggc gcagggcgag agcacgtgag aaggaatttt
    16141 ctggtgacac ggcacttaag cgattacctg accctgatga ggttgtttgt cgcgcggtga
    16201 gcaagcaggc cattgaatcg catggctcaa aggaaagtcc gtgatggggg aagcagtatg
    16261 ggagcaggtc caggaacagt gctccgacgt gcgcaaggtc gatctcctgg tcgcccttcc
    16321 gtcgttcaat caggcctcaa ccgttgtgcc cgccgtacgc acgattgtgg cagggctgaa
    16381 atccttcttt gggcacgctt ccatcctctt cgtgaacgcg gatgtcggct ctcaggatgg
    16441 aactcctgat cttgtcaggc agacggtcgg cgacttgttg ccctttgcgt cgatccgtcc
    16501 gaacaccgaa agcatgtccg gcagtacctt cgccttgaat cgattttcct actccggcat
    16561 gccggcacgg gaggaggcct tccggtcagt gtttgccgtc acgcggaggt tggaagcccg
    16621 ggcctgcatc gtcttggact caagcgtacg atcggttaca gctgaatggg tttctctgct
    16681 cgcacaaccg atattggaga aaggcgttga ttatgtggcg cccgtgttca atcggcaacg
    16741 atatgaaggc agcctgacca acaatctcat cgcccccttg actcgggcgc tttatggaaa
    16801 gcgggtcgct tgtcagatcg gtggcgggta tggcttttcg caaaaactct cgagcttcct
    16861 tttagagaac gaagcgctat gggaaggaga tgccggccgc ttcgccattg acagctgggt
    16921 gaccaccgtc gccatcgccg agggatacca ggtttggcaa gcctgtcttg gagccaaggt
    16981 tcaggatatt aaagccggag cggctgatat ctcgacgagt ctcgcccaag ccgtaggcgc
    17041 gaccttccac tatatggagc gatatcagga tatttgggag aggcaggttg gctctgaaga
    17101 ggtcccggtc gtcggtcgcc cgattgagat tggcatggaa tcggttccaa tccaagtgga
    17161 tcggatgctc aagggatttc gtcagggatt gagggacctg cttccgattt gggaggttat
    17221 acttgcacct gacacgttgg ccggcgtgct ggagttgggg ttgagggatg acgaggaatt
    17281 tcgcttcccc cttccgctgt gggttcagac cgtctatgac tttgctatcg cgtatcacga
    17341 gaaggtcatc catcgtgagc atctcttgaa gtccctggtg ccgctgtatc tcggtcggac
    17401 cgcgtcagtg gtccttgaaa cgaaaggcgg cggaccggga ctggtccagg atacggtcga
    17461 acacggttgt caaacctttg aggtgacgaa gccgtatctg atcgaccgat ggaggttcca
    17521 atgagcgaga tgtggcagga tgcaatggtt gcggcctttc gggaaatggc gagccgaggc
    17581 gtgctgttct tgcccaaact catggccctg gtgacattct tgatgctcgg gcttgccata
    17641 ggatggctcg tcaaagtatc gctcatgcgc attctcaacg ttgtacgctt tgaagtgttc
    17701 tgcgaacgca tgggactctc tccagccttg acgaaggccg gcatgaaaca gcaaatcccg
    17761 cacctcgtcg gccgtctggc attttggatt gtatttctat tctttgcgtt catgggggtc
    17821 gatgcgttgg atttgcccgc gaccgccaat ctcatgagtg ccatcctagg ctttttgcct
    17881 aatgtgatcg ctgccattct ggtggcgctg cttggcattc tcctggctaa tttcaccgga
    17941 gaggcggtct tgatcgccgc agtcaacgct caaattcatg gcgcccggtt catcgccaac
    18001 atggttcgct ggggcgtggt catttttact gtcgccatgg tcctgacaca gcttgggatt
    18061 gcaaaggaga tcgtagtggc agcgttctcc atcatctttg gaggcatcgt tttggcgatg
    18121 gccatcgcgt tagggttggg cgggcgcagc atcgcgaagg aggtgctcga gcgtcgcttc
    18181 ggtcgcgaaa agagcaggga cgagttcact cacatttaaa tgggcggcag acgaaccaac
    18241 agtcatgaca catcaagacg gaagacaacg ggttgccatc gagggagtcc gaccggaaat
    18301 tgacggggga agattcccga tcaagcggac agtcggcgaa gagatcgtgg tcgaggccga
    18361 tgcctttgcc gacggacacg acgtcgttgt tgtgatcctc cgttatcgcc tcagggactc
    18421 cacggagtgg acggatctgc ccatgaagca cgtcggtaac gaccgttggc gtgcggcgtt
    18481 tcacgtgagc acgatgggac aatggcacta cacgatcacc ggctgggtcg atcactttga
    18541 aacctggcgc cgggacctgg tcaaaaagct cgacgccgga cagaatctgc aagttgacct
    18601 gcttatcggg gccaaactca tcgatagcgc ggtcgctcga gcttcggcgg acgatgggcg
    18661 gaagctcacg gcgtggaggc aggcgttgag atcgaatgaa tctcctgacg acgaccgcat
    18721 tcgtatcgcg ttaagcgagg acctccgagc gctcatggcg agatacccgg atcggcgcct
    18781 cgcttccgtc tatgaacgag aacttcgggt aatcgtggac cggcaaaagg cgcggtacag
    18841 cacctggtat gaattgtttc cccgctcctg gagccgcacg cctgggcagc acggcacatt
    18901 caaagactgc gaagccgcgc tcccgtatgt cgcttcaatg ggattcgatg tgttgtattt
    18961 gcctccgatt catccgatcg gccaaacgca ccggaagggt aagaacaatg cgccgacggg
    19021 gggcgctgca gatcctggca gtccgtgggc gatcggcgca ctcgaaggag gccataagtc
    19081 catccatccg gaactaggta cgctgcaaga ctttcggcga ttgagagagc aggcaagcac
    19141 ctatggcatc gagatcgcga tggacgtggc ctttcaatgc tcccctgatc atccctacgt
    19201 caaggagcac cgtgaatggt ttaggattag gccggacggc actgtgcaat acgctgagaa
    19261 tcctccgaaa aaatatcagg atatatttcc cctggagttt gagaccgacc agtggcaggc
    19321 attatgggat gagttgaaga gcgtcatctt gtattggagc aatgagggtg tcagagtctt
    19381 tcgtatcgat aatccacata cgaagccatt cccgttctgg gaatgggtga tcagcgagac
    19441 gaaacagatc tatcccgatg tgatctttct cgctgaagcg ttcgcgaggc caaaagtgat
    19501 gctgcggctc gcgaagcttg gctttacgca gtcctacaat tattttccct ggcgcaccac
    19561 gaagtcggag cttacgcagt acttcactga gctgacgcag accgatatcc gcgaatactt
    19621 tcgcccgaac ctctggccca atacgcctga tattcttacc gagcagctgc agcacggcgg
    19681 ccgagccgcg ttcatgtctc gcttcgtcct ggcggcgacg ctcggtgcga gctacggcat
    19741 ctacggtccc gcatttgaac tgggagagtg cgtgcctcgg gaagtgggaa gcgaggagta
    19801 tctgcattct gaaaaatatg agatcaagca gtgggatctt cgccgagagg acagtctcag
    19861 cgagtttatt ggtcacgtga atcgcatccg gcgcgagaac ccagcccttc aaagcgactg
    19921 gagtctgcat ttccatgaag tgggaaacga ccaattgctg tgttacagca agcggacgtc
    19981 tgacggggcg aacgtcattc tggtcgtcgt caacttgagc ccttaccacg tgcattcagg
    20041 atggctcgag ctggatttgg cgcatcttgg tgtcgagcta gaccgtccat tccaagttca
    20101 ggacctgctg acgaatgcgc attatctgtg gcagggacgg cgcaactata tcgagatcaa
    20161 tccccattcc gctccggctc atattttccg cgtcaagggg aaggtacaca gcgagcggga
    20221 tttcgactat tacttctaac agaggccggg cttcccgact gtgaaacaat gttaactgcc
    20281 aagcatgact gggcacgtct cttagaagga gacgctgcag cggacttgat gcgagttttg
    20341 tgcgaaattc tgcagacgcg gcgttggttt ggtggaaagg cccgccggat cgccgaagtc
    20401 cgtatcgtgg actccattgc ggtgccctcc gattcgagtt cgatccttct tttgctctgc
    20461 gtcgactatc gggatggcga gagcgagatc tacagtcttc cggtgacggc ggtattcggc
    20521 gaggatgctg agcgcgttca gagggagata ccgcgagccg tcatcgctcc gttcatgctt
    20581 acaaaaaacg gcacgaccga catcgggatc ttgtacgatg cgctctggaa tcgggaattc
    20641 acagccgaac tgttggccgc gctgcgcaac gagtcacgat tccaaggcgc tgtgggagct
    20701 gtcacggctt cagtgacggc cgccttcaag gacctcgtgc cctcaggaat ccgtctggac
    20761 cctcgtgtca tggggtcaga gcagagcaat acgtcggtag tctacgatgg ccatgtcatt
    20821 ttgaaactct atcgacgcct tgaagagggg atcaatccag acctcgagat cggccgtgtc
    20881 ttgacgcgga tgcagtttcc gttcgtgccg gctctcgccg gtgccattga gtaccagagg
    20941 ccgtcgcgag aagtctcgac gcttggagtg ttgcaacgat atgtcgataa cgccggcgat
    21001 gcctggacgt ggagtcttca aacggtgaag gaattcttca cgcgcgttgg ttccctgtct
    21061 ctggacaggc acgctacggt tgataaatcc tccctgctcg agctggcagg cgagccgtat
    21121 ccgacgccgg tgcgccattt catggaggga tatctggagt ccgcggagcg gttgggtcag
    21181 cggacggcac agcttcatct ggcactcagc cgggtggagg atgatcccgc ctttgccccg
    21241 gaaccgctca ccttggagta ccgacaggct cggtacgagg ctgccctacg aggcatgaca
    21301 agcacactgg cgcttgtgaa agcccgcatg gattcgctgc caacggctgc gcaagagcag
    21361 gccaatgttc tctttcggtt ccgtcccacg ctcgagcgca tctttgacgc cttccggaat
    21421 ctggacaccc cgggttcgct gatccgttgt cacggcgact atcacttggg gcaagtattg
    21481 tttacgggag ccgattttgt cattactgat ttcgaaggag agcctgcaag accgttagcc
    21541 gaacgccgca tgaagcatcc gcctctcgtc gatatcgcag gcatggtccg gtcatttcaa
    21601 tatgtgccat tcgcattttt aaaaggtgca ggccttccgc cttcaccgtg gtgtccattt
    21661 tggtcaaatt gggcctgtgt cgcattcctc aaaggctacc tgagcgcggc gaccggctca
    21721 gaactatggc cgcaaactaa cggcgacgtc cgtccgttat tagatgcgta tatcgcagag
    21781 aaggctttct atgaattgca gtacgagctc aataacaggc cggattgggt tgacataccg
    21841 ctccatggtc tcgcggcgat tctcaacata cgagaagaaa gcgaaaggtg atcacgtgcg
    21901 gctactcgag gtagggagtc accatgacga gaattcagtt tacacccagg aagcctggta
    21961 aggagatgac attgctaacg aaagatgatt tgtacctgtt caacgaaggc acgcattttc
    22021 atttgtacga gaagttagga gcacatccgt tggagtctga ggggacgagg ggaacgcatt
    22081 ttgccgtatg ggcacctaat gcagagcacg tctcagtcat cgggaccttt aacaattggc
    22141 aaagaggcgc acatgtcctt
//
LOCUS       DASTQY010000017         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_590402, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000017 DASTQY010000000
VERSION     DASTQY010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590402"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..604)
                     /locus_tag="VFR82_00620"
     CDS             complement(62..604)
                     /locus_tag="VFR82_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU4500129.1"
                     /translation="MEMTLLLNSTYEPLRVVHWQKAIALLWQGKVEVLEVYDREIHGV
                     SISFKLPAVMRLLKLVRLKDSHRAVKFSRINIFTRDGYVCQYCRTKFRTEELTFDHVV
                     PIAKGGRKTWENIVTACWRCNNRKSGRTPDEAGMRLMKRPVKPRWSPIVTITIGIRNT
                     PESWRDYLYWNMELDADSGP"
     gene            769..1098
                     /locus_tag="VFR82_00625"
     CDS             769..1098
                     /locus_tag="VFR82_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU4500130.1"
                     /translation="MSEFVTVATTQDIPPGTGRTVEVQGVWIALFNVDGSFFAVDNTC
                     PHAGGPIGEGSLSGDIVTCPWHGWQFNVRTGERSGNPNFTVACCPVRVEGEQIQIALP
                     PDFTSNC"
     gene            complement(1112..1570)
                     /locus_tag="VFR82_00630"
     CDS             complement(1112..1570)
                     /locus_tag="VFR82_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500131.1"
                     /translation="MVKILHYVVLGVILLGAVYYVWTKPPTINPMIDRRSADALAMVQ
                     THRALGHPTILQAMTEHVRSMKDRGLNARLGEWRVKQVDGDLYEVRVQLRDKGVSGQW
                     FEREFIWHANLSLKRVNAASLPADGITPKPAETDEPTVPSPPKPNILGPA"
     gene            1819..2262
                     /locus_tag="VFR82_00635"
     CDS             1819..2262
                     /locus_tag="VFR82_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEU4500132.1"
                     /translation="MKFFLYADNLHPTQLKRRAPEHQFQMLATLPDHTIQFCRWSSQW
                     RCGLASVVPSPGEKVWGAIFELTDEDIKLLDQFEDDVPQEAFRHVQVTVLSEAGEKIL
                     VTTHAATPIGKFKPKEHYLDWVIKGLKHWKFPEDVIEQWQAYRPH"
     gene            2332..2658
                     /locus_tag="VFR82_00640"
     CDS             2332..2658
                     /locus_tag="VFR82_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEU4500133.1"
                     /translation="MPKPKYHIVVCTNARPPGHPKPSCGSAGSAQLLMAFNMGLMQRG
                     VMPGDVLVSGSSCLGPCEQGPTVVVYPEGTWYSKVTEADVATILDEHIKGGKPAAKLN
                     PDAVWK"
BASE COUNT          670 a          874 c          840 g          604 t
ORIGIN      
        1 caagaagccg gccggaagaa gtatgcacaa caggcgtgat ggacagcgcg atagaaggtg
       61 attacgggcc agagtcagcg tcgagctcca tattccaata caaataatcc cgccaactct
      121 caggggtgtt ccgtattcca atggtaatcg tgacgatcgg actccagcgc ggcttcaccg
      181 gccgtttcat cagccgcatg ccggcttcat ccggcgtcct gccgctcttg cgattattgc
      241 agcgccagca ggccgtcaca atattctccc acgtcttccg tccgcccttg gcaatcggca
      301 ccacatgatc gaaggtcagt tcctcggtgc ggaacttggt gcggcagtac tggcagacat
      361 acccgtcgcg ggtgaagatg ttgatacggg aaaatttcac ggcccggtgg ctgtccttca
      421 gtcggaccaa cttgagcagc cgcatgaccg ccggcagctt gaaggaaatg gagacgccgt
      481 gaatctcgcg gtcgtagact tcgaggactt ctaccttacc ttgccagagg agagcgatgg
      541 ctttttgcca atgaacgaca cgcaggggtt catacgtaga attgagcagg agggtcattt
      601 ccattcaaca acctcattcc caaccgaggc tcctgttcga tgaagggcct tgtatcggtg
      661 tggggtacgc tcgcgtatgt ctatgccata agattgcgcc gaaatcaagc gcggaggcca
      721 ttctgcggga ttgacgacgg ggacataaac acgattatgt gaggctgcat gtctgagttt
      781 gtcacagtcg cgacgacgca ggatattcca cctggaaccg gacggaccgt cgaggttcag
      841 ggagtatgga tcgccctctt caatgtagac gggtcgtttt ttgcggtcga caatacgtgc
      901 ccgcacgcgg gaggaccgat cggggaaggc tcgctctcgg gagacatcgt gacctgccca
      961 tggcacggct ggcaattcaa cgtccgcaca ggcgagcggt ccggcaatcc caactttacc
     1021 gtcgcctgct gtccggtccg cgttgagggc gagcagattc agatcgcgct gccgccggac
     1081 ttcacatcaa actgttgaag gaagtgcgac gctacgcagg acccaaaata ttcggttttg
     1141 gagggctcgg tacggtgggc tcgtcggtct ccgccggctt cggcgtgatt ccgtccgccg
     1201 gcaacgacgc cgcattcact cttttcagcg acagattcgc gtgccagatg aattctcgtt
     1261 cgaaccattg cccgcttaca cccttgtccc gcagctgcac acgcacttca tagagatcgc
     1321 cgtccacttg cttcacgcgc cattcgccga gccgcgcgtt taatccccgg tctttcatgg
     1381 aacgcacgtg ctcggtcatc gcctgcagaa tggtgggatg gccaagcgcc cggtgggtct
     1441 gcaccatcgc caacgcatcc gcgcttcgcc ggtcaatcat cggattgatc gtgggcggtt
     1501 tggtccagac atagtacacg gcgcccaaca ggatcacgcc gagcacgacg taatggagga
     1561 ttttcaccat actcaaacga cctttcatct ctcagaaagc gagccgcatc ttaggaagtg
     1621 gtctcacgcc aagtcaatca gacgaattga cgcgccggcc gagcaccaga tcgatctcaa
     1681 agcagttgtt caaaatgggt cgtcggcaag gctgacgccc cttttcaaca attgccagag
     1741 cttgtgccag tgaaagtggg tatgctagaa gaataccccc taacacattc agcactgtga
     1801 tgaggccata caagagttat gaagttcttt ctctacgccg ataatctcca tccgactcaa
     1861 ctcaagcgca gggctccgga acatcagttt cagatgctgg ccaccctccc cgaccatacg
     1921 atccagttct gccgctggtc gtcccagtgg cggtgcgggc tcgccagcgt cgtcccgtcc
     1981 cccggcgaga aagtctgggg cgcaattttc gagttgacgg acgaagacat caaactgctc
     2041 gatcaattcg aagacgacgt gccccaggaa gccttccgtc acgtccaggt gacggtcctc
     2101 agtgaagccg gagaaaagat cctggtgacc acccatgcgg cgacgccgat cggaaaattc
     2161 aaacccaagg aacactatct cgattgggtc atcaagggct tgaagcattg gaaatttccg
     2221 gaagacgtga tcgagcagtg gcaagcctat cggccccatt gatcgatgtg gtcgcatacg
     2281 attaagctca gacgggagca cagcgcagta aacacttagc gtgaggagca catgcccaaa
     2341 ccaaaatatc acatcgtcgt ctgcacgaac gccaggccgc cgggccatcc caagccctca
     2401 tgcgggagcg caggatccgc gcaactgctg atggcgttca atatgggact tatgcagcgt
     2461 ggtgtgatgc cgggcgacgt tctcgtcagc ggctcttcct gcctgggacc ctgcgagcag
     2521 ggacctacgg tcgtcgtcta tcccgagggc acctggtact cgaaggtgac ggaagccgac
     2581 gtcgcgacca tcctcgatga acacataaaa ggcggcaaac ccgcggccaa gctcaatccc
     2641 gatgccgtct ggaagtagct gtaccattga tccttccttt ttctaggatg ctcaaaatgg
     2701 acttgaggcc aggccgcagc gagcgaagat tcgaggcgta cgcgatatca cccgccccac
     2761 ccatcgcctg tcagaacagg cgtttgccca agcagtacgt tgaggatctg agcgatgcga
     2821 gaacgcagcc cggtgaaggg cgcgttccga cgcgccgggg tgggcgggtg agaaagaata
     2881 ttttcagcat cctatgatga ctgtggcaca ctccactcat cacgagcata aatcccacgc
     2941 accccggaag atcgggtgca tggtgattac ctgcagcgac acccggac
//
LOCUS       DASTQY010000018         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1203250, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000018 DASTQY010000000
VERSION     DASTQY010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203250"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFR82_00645"
     CDS             <1..477
                     /locus_tag="VFR82_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4500134.1"
                     /translation="LVQHEARIRKRITIATSLAVGALVVQVDQNQMRQVFWNLATNAF
                     DAMPEGGQLTISTGCRHVDAAGRSGDVVEIAFQNTGESIAQDNLDKIFLPFFTTKKEG
                     SGLGLAAVHRIVDLHGGWIKVESQPQRGTCFVVCLPRSGDDGVRLWHEGRTPWKRS"
     gene            462..1844
                     /locus_tag="VFR82_00650"
     CDS             462..1844
                     /locus_tag="VFR82_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU4500135.1"
                     /translation="MEKILVVDDEKSLREVMSIMLKRAGYEVTEASDGEQAIGQVNKE
                     IYDLVITDLRMPKADGMDVLKAVKSSSPETVVLVVTAFGTADSAVEAMKQGAYDYLTK
                     PFQVDEVQLIIRNALEKRRLSTENMLLKREMASQSSFAQIVGQSEAMQKVFDVVRKVA
                     DSKSNVLICGESGTGKELVARAIHYNSARSPMPFVAVNCSAVPETLLESELFGHMKGS
                     FTGAVANKAGLFEIANGGTIFLDEIGDTTPAIQVKLLRVIQEREFRRVGGNQDVKVDV
                     RVIAATNRELEKAVADGSFREDLYYRLDVIPIRLPPLRLRAGDIPLLVAHFLEKFSQE
                     SGKAAPTLTPEAIHVLLGHEWRGNVRELENLIERVVAFSTGTVSDTDVRGWLHHALAP
                     QQSQGVPTDLPEDGLDLEGLINGIEKDLLMRALERSKWVKKKAARLLKLNTRSFRYRL
                     EKYAIKGGRD"
     gene            1837..>2382
                     /gene="ispE"
                     /locus_tag="VFR82_00655"
     CDS             1837..>2382
                     /gene="ispE"
                     /locus_tag="VFR82_00655"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEU4500136.1"
                     /translation="MTNRESGSFQSGPSSITVHAPAKINLILRILDRRSDGYHNLWSV
                     MQTVALEDELEIRAASPGGVRLTCDTVRLPVDSSNLVHRAASAVLDQASLSVGLDVEL
                     RKRIPMGAGLGGGSSDAAATILGLNQLLKLGWSREQMQSVAEQLGSDVPFFLFAPAAV
                     VSGRGAAVKPVWMDESRWVVLV"
BASE COUNT          536 a          628 c          731 g          487 t
ORIGIN      
        1 ctagtacaac atgaagcgcg gatcagaaaa cggatcacga tcgcgacctc attggccgtc
       61 ggagccctgg tggtccaggt ggaccagaat caaatgcgcc aagtgttttg gaacctcgcc
      121 acgaacgcgt tcgacgccat gccggaaggc gggcaactca ccatttcgac agggtgtcgc
      181 catgtcgacg ccgccggacg aagcggcgac gtcgtcgaaa tcgccttcca aaacacgggc
      241 gagagcattg cgcaggacaa tttagataag atctttctcc cattctttac gacgaagaaa
      301 gaaggctcgg gcttggggct ggccgccgtc catcgcattg tcgatctgca cggcggctgg
      361 atcaaggttg agagccagcc gcaacgggga acatgctttg tggtgtgtct ccctcgctcg
      421 ggggacgatg gtgtgcggtt atggcatgag ggacgaacgc cgtggaaaag atcctagtcg
      481 ttgatgatga aaagagtctc cgggaagtca tgagcattat gctcaagcgc gccgggtacg
      541 aggtcaccga agcctccgac ggggagcaag cgatcggcca ggttaacaaa gagatctacg
      601 atctcgtcat caccgacctc cgtatgccaa aagcggacgg aatggatgtg ctgaaagcgg
      661 tgaagtcctc ctcacccgaa acagtcgtgt tggtcgtgac tgctttcggc acggcggatt
      721 cggccgtgga agcgatgaag caaggggcct atgactatct caccaagccg tttcaggtag
      781 acgaagtcca gctcatcatc cgtaacgcac ttgaaaagcg ccggctgtcc acagagaaca
      841 tgctgctcaa gcgtgaaatg gccagccagt cctccttcgc gcaaatcgtg ggacagagtg
      901 aggcgatgca aaaagtgttt gacgtcgtgc gcaaggtggc ggactcgaaa agcaatgtgc
      961 tgatctgcgg ggaaagcgga acggggaagg aattggtcgc ccgggcgatc cattacaaca
     1021 gtgctcgcag tcccatgccc tttgtcgcgg tcaattgcag tgcagtaccc gaaaccttac
     1081 tcgagagtga gctgtttggt catatgaagg gatcattcac aggcgcggtg gcgaacaaag
     1141 ccggtttgtt cgaaattgcc aacggcggaa ccattttttt ggacgaaatt ggcgatacca
     1201 cgccggccat acaggtaaag ctccttcgag tcattcaaga gcgggagttc cgccgagtgg
     1261 gcggcaatca ggacgtgaaa gtcgacgtgc gggtgattgc ggcgacgaac cgcgagcttg
     1321 aaaaagctgt ggccgatggc tccttccgag aagatctcta ttatcgtctc gacgtcattc
     1381 ccattcgcct tccgccgctg cgcctcaggg ccggcgacat tccgctgctc gtcgcacatt
     1441 ttctcgagaa attctcgcaa gaaagcggga aggcagcccc aacgctgacg ccggaggcga
     1501 tacacgtcct tttggggcat gaatggcgag gcaatgtgcg ggagctggaa aacttgatcg
     1561 agcgtgtcgt tgccttttcg acgggcaccg tgagcgacac agatgtccgt ggatggctcc
     1621 atcacgcgct cgcacctcag caatcacaag gggtaccgac ggatctcccg gaggatgggc
     1681 tggatttgga agggctcatc aacgggattg agaaggatct cctgatgaga gcgttggaac
     1741 gttcgaaatg ggtcaagaaa aaagcggccc ggctcttgaa gctgaacacc agatcgttcc
     1801 ggtaccggtt ggaaaaatat gctataaaag gaggtcgtga ctaaccgcga gtcaggatcg
     1861 tttcagtccg gcccttcctc aatcacggtt catgctcccg ccaagatcaa tctcattctt
     1921 cgcatcctcg atcgccgctc cgatggctat cataatctct ggtcggttat gcagacggtc
     1981 gcgctggaag acgagttgga aatccgtgca gcttccccgg gaggggtccg cctgacctgt
     2041 gacactgtcc gcttgccggt agattccagc aatctcgtgc atagagcagc atctgccgtt
     2101 ctcgatcaag cctctctctc cgtcggcctc gacgtggagc tacgcaaacg gattccaatg
     2161 ggagcggggc tcggcggagg cagcagtgac gcggcagcca ccatccttgg cctcaatcag
     2221 ctgttgaagc tgggatggtc gagagaacag atgcagtcag tcgcggaaca gttgggcagt
     2281 gatgtgccgt tctttttatt cgcacctgcc gcagtggtct ctgggcgtgg tgcggccgtg
     2341 aaaccggtgt ggatggatga gagccgctgg gttgtgctgg tg
//
LOCUS       DASTQY010000019         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1595774, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000019 DASTQY010000000
VERSION     DASTQY010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595774"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VFR82_00660"
     CDS             <1..896
                     /locus_tag="VFR82_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU4500137.1"
                     /translation="DIIKKNPHLEWVASNPTESFRWQTHDFPCSIARWNYHPENEIHL
                     IRQSTGKAFVGDYIGEFGPGCLTAVGPNLPHNWVSDLEPDEVVEERDVVLQFDAALFM
                     RAGAIFPEAKEIVPLLNAWQRGLQFHGETARQGAMLMEEIGKSYGFHRLVLFLELVRR
                     LAQSSERTPLASADYVPNLDQDAPRIIQKVMSYILDNFKDTVSLSEAAEIAGMGESSF
                     SRFFKKNSGNNFVDYVRKLRVSHACRILSETNMPVTEVCFEVGYNNISNFNRHFLAEK
                     GVTPSQYRRMSARKFTAEASG"
     gene            1144..1866
                     /locus_tag="VFR82_00665"
     CDS             1144..1866
                     /locus_tag="VFR82_00665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4500138.1"
                     /translation="MKLKDKVAVVTGAASGIGLASTEAMLAAGSRVVMVDRDEAALKA
                     LRDKHGDAVIPLVIDLLDPKDCATLLPRVLEKAGQLDILHANAGTYVGGDLVDADTGA
                     IDRMLNLNVNVVMKNVHDVLPHMIERRTGDIIVTSSLAAHFPTPWEPVYASSKWAINC
                     FVQTVRRQVFKHGIRVGSISPGPVISALLADWPPEKLKEARESGSLLEASEVANVVMF
                     MLTRPRGMTIRDVVMLPTNFDL"
BASE COUNT          472 a          587 c          638 g          440 t
ORIGIN      
        1 ctgatataat aaagaaaaat cctcatcttg agtgggttgc atccaatcca acagaatcgt
       61 ttcgttggca gacgcatgac tttccctgca gcatcgcgcg gtggaactat catccggaaa
      121 atgaaatcca tctgatacga caatcgaccg gaaaggcttt cgttggtgat tacatcggcg
      181 aattcggtcc aggctgcctc acggcggtcg gcccgaacct gccacacaat tgggtcagcg
      241 atctcgaacc tgacgaggtc gtggaggaac gggacgtggt cctgcagttt gatgcggccc
      301 tcttcatgcg cgctggcgcg atctttccag aggccaagga gatcgttccc ttgctgaacg
      361 cttggcagcg tgggctccag ttccatggag aaacggcacg tcagggggcc atgctgatgg
      421 aggaaattgg gaaatcctac ggttttcacc ggctggtgct gtttctcgaa ctcgtccgcc
      481 gtctcgccca gtcgtcggag cgcacaccgc tcgccagcgc cgactatgtg ccgaatcttg
      541 accaggatgc tcctcggatc attcaaaagg tgatgtccta catactggac aacttcaagg
      601 acacggtcag cctgtctgaa gctgcggaga tcgccgggat gggagagtcc agcttttccc
      661 gcttcttcaa aaagaattcg ggcaacaact tcgttgatta tgtgcgaaag ctgcgtgttt
      721 cccatgcctg cagaattctg tcagagacaa acatgccggt cacggaagtc tgctttgagg
      781 tcggctacaa taatatttca aacttcaacc gacacttcct tgcggaaaag ggcgtcaccc
      841 cttcccaata ccgcagaatg tcggcccgga agttcacggc cgaggcgtcc ggatagccac
      901 gaccactgcg tccaacctgc cctggggccg ccaagggatc agacttttag gatctctgcc
      961 aacctgtatg ccattgcaga ccaagcagta caaatctgat attgttgcaa tgcacaaagc
     1021 aaactcatac agattgttag caagggcgtg gctagttcgt caggccccag gggtgataaa
     1081 acctcctcag ccggatccac caatggtcgg ctgaatgaca aaatataatc gagggagccg
     1141 acaatgaagt tgaaggacaa ggttgccgtc gtgaccgggg ctgcgtcggg catcggattg
     1201 gctagcaccg aggcgatgtt ggcggcaggc tcgcgcgtgg tgatggtcga ccgcgacgag
     1261 gccgcgctga aggcgctccg cgacaagcac ggcgacgcag tgatcccgct ggtcatcgac
     1321 ctgctcgacc cgaaggactg cgccacgctg ctgccgagag tcctggagaa ggccggccaa
     1381 ctcgacatct tgcatgcaaa cgcgggtacg tacgtcggcg gcgacttggt cgatgccgac
     1441 actggggcta tcgaccggat gctgaacttg aatgtcaacg tcgtgatgaa gaatgtgcac
     1501 gacgtgctgc ctcacatgat cgagcgtcgg acgggcgaca tcatcgtcac gagttccttg
     1561 gcggcccatt ttccgacgcc atgggagccg gtctatgcgt cgtccaagtg ggcgatcaac
     1621 tgctttgtcc agacggtgcg acgccaggtg ttcaagcacg gcattcgcgt gggctcaatc
     1681 tcgcccggcc ccgtcatcag tgcactgctc gcggactggc cgccggagaa gctgaaggaa
     1741 gccagggagt cgggaagtct gctggaggcc agcgaagtgg cgaacgtggt gatgttcatg
     1801 ctgacgcggc cacgcggcat gacgattcgc gacgtggtca tgctgcccac caatttcgat
     1861 ctctaggggg catcctcatt cgaaaggcgt gagagaggcg cacagctcgc agttccgccg
     1921 tctctcaaaa cccattcttg attcctgcct ctcaaaagat tgcggatccg caaatttgga
     1981 gcgggtagag tcgagaacgg gcgcagtggc ttgggccgat ggtggcaact ggatgagctg
     2041 accggcgcat ttcttttcat cggccatggt ggtggagaca gggaaggaaa tgcaatggcc
     2101 atggaacttg ggctcgacgg caagatcgcg gtcatca
//
LOCUS       DASTQY010000020         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1192920, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000020 DASTQY010000000
VERSION     DASTQY010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192920"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFR82_00670"
     CDS             <1..290
                     /locus_tag="VFR82_00670"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEU4500139.1"
                     /translation="AAIPGYIDYRSLTDPVTTRVAQMHMVINVGLVVLYTVNAGLRVL
                     NGPEAVLPLILSFLGVAALGVSGWLGGELVYVQGVAVQPERESSGPRRRAA"
     gene            391..2142
                     /locus_tag="VFR82_00675"
     CDS             391..2142
                     /locus_tag="VFR82_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU4500140.1"
                     /translation="MADTVADLLVDRLIDWEVDTVFSLPGDGINGIFESLRRRQGRIK
                     VIQVRHEESAAFAACGYAKFTRRLGVCLATSGPGGLHLLNGLYDAKCDGQPVLAITGH
                     TFHDLIGTHYQQDVDLVKVFADVAAYNERVMGPAHVCNVVDEAIKTAISRRTVAHITI
                     PKDIQDWSAEGRRSKANVPMHSGDLYSEALPLPPHAMLQQAADILNGGSRVAILAGRG
                     CLTARKEIVAVAETLAAPIVKPLLGKAVVPDDHPYTTGGIGLLGTAPSQEVLEECDTL
                     LIAGSSFPYLEFYPKPGQARAVQIDLDPSRIGLRCPVEVGLVGHCWDVLRALLPLLTR
                     RSDRTFLSKTQERMQQWNDLMEMRGTRNDVPLKPQVVARALNEFLDRDAVICSDTGTV
                     TTWAARHIMIREDMEFSVSGTLATMANALPYSIGAAVAFPGRQVVCFTGDGGMTMLMG
                     ELATIAKYRLPVKVIVIKNNVLGMIKWEQLAFEGNPQYGVQLHPIDFVSVARACGVSG
                     YSVDDPALVRAVIGEAFHHPGPALVEASVDPNEPPLPGKITTDQAWQFAKALAKGQKD
                     RWDIIKTIVENKIREVV"
     gene            2240..>3512
                     /locus_tag="VFR82_00680"
     CDS             2240..>3512
                     /locus_tag="VFR82_00680"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEU4500141.1"
                     /translation="MAHGISKAIVIKNGNLFFLAPPEGEVPMARGHGFGLYYHDCRYL
                     NGYELLVGGEHPEPLAARSWEGGHAVFQLTTPDFQAPDGRMIDRETVGLKWERVIDAE
                     QCVLHEQLDVRYFGYEAVDFPITMRFRAGFEDVFEIRGLIREVKGKLFKPYWKDKCLY
                     FSYEGGDRIRRSLAVHFYPLPTQVKGNAVQFDIALKPNETKQIRISLALSESEEQPNR
                     LFAQTSVYRDLHAVTQGIKASCEQWVRRETEFHSDSLLLNRVMRRSLMDLHMLKTSIG
                     GDEFFAAGVPWYVTLFGRDCLVTSLQTLAFWSDTAAATLRLLARYQGAEVNDWRDEEP
                     GKILHELRVGELARMNKIPHTPYYGTIDATPLFLILMSRYAHWTGDLGLFEELRTPIE
                     RALHWIDKYGDIAGNGYVSYVGNTNKGLLNQG"
BASE COUNT          731 a          980 c         1079 g          722 t
ORIGIN      
        1 ccgctgccat cccgggatat atcgactacc ggtcactgac agatccggtc acgacccgcg
       61 tggcacagat gcacatggtc atcaacgtcg ggctggttgt gctgtacacg gtcaatgccg
      121 ggttgcgtgt cttgaacggc ccggaggctg ttttgccgct gattctctcg ttcttaggtg
      181 ttgccgcgct cggcgtgtcg ggttggttgg ggggtgagct ggtgtatgtg cagggagtcg
      241 ccgtccaacc ggaacgggaa tcgtccggtc caaggcgacg agcggcttaa ggaacaacaa
      301 acctctgcat gccggatcct cgcacatcag aatagatgca agccggcagg cacatagcgg
      361 cagtcatcgc tgctcgaatg ggagattgat atggcagaca ccgttgcaga tctgttggta
      421 gatcggctca tcgactggga agtggacacg gtgttcagcc ttccaggcga cggcatcaac
      481 ggcatttttg aatccctccg gcgtcggcag ggccgcatca aagtcattca ggtgcgccac
      541 gaggagtccg ccgcctttgc ggcatgcggc tatgcgaaat tcaccaggcg gctcggcgta
      601 tgcctggcga catccggccc tggcggactg cacttgctga acggattata tgacgccaaa
      661 tgcgacggcc aacccgtgct ggcgattacg ggccatacgt ttcatgatct gatcggtacg
      721 cattaccagc aggatgtcga tctggtcaaa gtcttcgctg atgtcgcggc ctacaacgaa
      781 cgggtcatgg ggccggccca tgtctgcaac gtggtggatg aggccatcaa gaccgccatc
      841 tccagacgga cggtggctca tatcacgatt cccaaggaca tccaggattg gtctgccgag
      901 gggcgtcgat ccaaggcgaa cgttcccatg cacagtggcg atttgtacag cgaggcgttg
      961 ccgctgccgc cgcatgccat gttgcagcag gccgccgata ttttgaacgg ggggtcgcgg
     1021 gtggccattc ttgcgggccg cggttgtctg acggcacgaa aggaaatcgt ggccgttgcc
     1081 gagacgcttg ccgcgccgat cgtcaaacca ctgctcggca aagcagtggt gccggacgat
     1141 catccctaca cgacgggggg aatcggcttg ctcgggaccg caccgtcgca agaagtcttg
     1201 gaagaatgcg acacgctgtt gatcgccggc agcagcttcc catacctcga gttctatccc
     1261 aagcccggcc aggctagggc cgtgcaaatt gatctcgatc cttccaggat cgggcttcgg
     1321 tgtccggtcg aggtcggatt ggtcgggcat tgctgggatg tgctccgggc cttgttgccg
     1381 ctcttgaccc gccgatccga ccgcacattt ctctccaaga cgcaggagcg catgcagcag
     1441 tggaatgacc tgatggagat gcgcggcacc aggaatgatg tgcctctcaa gccccaggtc
     1501 gtcgcgcgtg cgctgaatga atttctggat cgcgatgcgg tcatctgcag cgacacgggc
     1561 acggtgacga cgtgggcggc gcgtcacatt atgatccggg aagacatgga attttcggta
     1621 tcgggcacat tggcaaccat ggccaatgcg cttccgtaca gcatcggggc cgcggtcgcg
     1681 tttccgggac ggcaggtggt ctgttttacc ggagacggcg gcatgaccat gttgatggga
     1741 gaactggcga ccatcgctaa ataccgcctg cccgtcaagg tgatcgtgat caaaaacaat
     1801 gtcctcggca tgatcaaatg ggaacagctg gcgtttgagg gcaatcccca atatggcgtc
     1861 caactgcacc cgatcgactt tgtctccgtt gcccgagcct gtggggtgag cggctatagc
     1921 gtcgacgacc ccgcactggt tcgtgcggtg atcggggaag cgtttcatca tcccgggccg
     1981 gcgttggttg aagccagcgt ggatcccaac gaaccgccgc tccctgggaa gatcacaacc
     2041 gatcaggcct ggcaattcgc caaagccctg gccaagggac agaaggaccg ttgggacatc
     2101 atcaagacaa tcgtggagaa caaaatcagg gaggtcgtgt aatgcgccga tgcgacgcag
     2161 caacgacatg atcagcggtg cttgatttct agagagagtc aaagagggac acaaccggtt
     2221 cggacgagga gtttcttctg tggcacacgg catttcaaaa gcaatcgtca tcaagaatgg
     2281 aaacctgttt tttctggcgc cccctgaggg cgaagtgccg atggctcgag gccatgggtt
     2341 cggtctgtat tatcacgatt gccgatatct aaacggctat gagttgctgg tcggcggcga
     2401 acatcccgaa ccccttgcgg cacgctcatg ggaagggggg cacgccgtgt ttcagttgac
     2461 caccccagac tttcaagcgc cggacggcag aatgatcgac agggaaacgg tcggtttgaa
     2521 gtgggagcgc gtcattgatg cggaacagtg cgtgctgcac gagcagctcg acgtccgcta
     2581 cttcggatat gaggccgtgg actttcccat taccatgcga tttcgcgccg gatttgaaga
     2641 cgtgttcgaa atacgcgggc tcatccgcga ggtcaaaggc aaattgttca agccctactg
     2701 gaaggacaag tgtctctact tttcctatga aggaggggat cgcatccgca ggagtctcgc
     2761 cgtgcacttt tatccccttc ccacccaggt caaagggaac gccgttcagt ttgatatcgc
     2821 gctcaagccg aatgagacca agcaaatccg catctccctt gccctctcag aatcggagga
     2881 acagccaaac cggctattcg cacagaccag tgtgtatcga gatttgcacg cggtcactca
     2941 gggcatcaaa gcgtcctgcg agcaatgggt gaggcgtgaa acggaatttc acagcgacag
     3001 cctgctgctc aaccgcgtca tgcgccggtc actgatggat ttgcacatgc tgaagacatc
     3061 aattggcggc gacgaatttt ttgcggccgg cgtgccgtgg tacgtcacct tgttcggacg
     3121 ggactgcctc gtcacgtcgc tgcagacact ggctttctgg tccgacacgg cggcggccac
     3181 actccgcttg ctcgcgcgct atcaaggggc ggaggtcaat gattggcggg atgaggaacc
     3241 cggaaagatc ctccacgagc tgcgcgtcgg cgagttggcg cggatgaata agatcccgca
     3301 tacgccgtac tacggcacta tcgatgccac tccgctcttc cttatcctca tgagccgcta
     3361 tgcacattgg accggtgacc tgggcctgtt cgaagagttg cgcactccca ttgaacgcgc
     3421 gctgcattgg atcgacaagt acggcgacat tgccggcaac gggtatgtca gttatgtggg
     3481 caacacgaac aagggcctgc tcaatcaagg gt
//
LOCUS       DASTQY010000021         5157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_786694, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000021 DASTQY010000000
VERSION     DASTQY010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786694"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="VFR82_00685"
     CDS             complement(<1..127)
                     /locus_tag="VFR82_00685"
                     /inference="COORDINATES: protein motif:HMM:NF017051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500142.1"
                     /translation="MHIVVVGLSHKTAPVEIREKLAVPESRLGEALTRLCAYQGIR"
     gene            complement(129..932)
                     /gene="ccsA"
                     /locus_tag="VFR82_00690"
     CDS             complement(129..932)
                     /gene="ccsA"
                     /locus_tag="VFR82_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEU4500143.1"
                     /translation="MAAVFFMVTMALYFAATVSFLALLLRRADALSTFALTITAVGFA
                     THTVALVMRMLTGPGASTPSIHEALSFFSWMLILVFLAVEFRHRLHVLGSFIVPLALV
                     SLVSAAALPETVPTLQPVFRTLWVHVTLSMLGTVGFAVAFVAGVMYLIQDGLLKSKRF
                     NVLYSKLPALDFLDDLNQQSIVLGFPLLTLGIITGAISAEFARGTYVNWNPEQTWALV
                     TWLFYFCVLVGRLTVGWRAKRAAYLTIIGFAGVVLTLVGVALKSFGSVS"
     gene            994..1734
                     /locus_tag="VFR82_00695"
     CDS             994..1734
                     /locus_tag="VFR82_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU4500144.1"
                     /translation="MARQKSQQTSRKASDSPNSRRRSGTLFIVGTPIGSLDDLTIRAR
                     AVLGEVSIVAAENPLVTQALLAKHRISATITSYGRRDENKIAVLLDRLKAGHDIALVS
                     DCGMPVIYDPGRLLITAARDAGYPVTVVPGPSAVTAAAALSGYSGDRLLFLGRVPRTR
                     PHLDQLCSTLKRESATAIMFVHPPILPHILNSIRRVLPNRAVTLAVNITKKDERVCQG
                     SATSLLEIARSLPQDAELTLVLAGVRKR"
     gene            complement(1742..1990)
                     /locus_tag="VFR82_00700"
     CDS             complement(1742..1990)
                     /locus_tag="VFR82_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEU4500145.1"
                     /translation="MNEQEQAVQPDAELDLRGVICPYNFVKTKLKLETMEPGQILSVI
                     LDAGDPIRNVPMSVSNEGHEVLSQEPLQQSYRVFIRRS"
     gene            complement(2137..2901)
                     /locus_tag="VFR82_00705"
     CDS             complement(2137..2901)
                     /locus_tag="VFR82_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500146.1"
                     /translation="MRKVLALGAAVLFVGSVSVAVAQERLQSNQSGMGVGEGVGDVSG
                     NSTRVNVFDRTSFLERLSQAETVYGRVLGVDLPGRKLHLESGGSSHDEGRAGGGAMNY
                     RTVYFDDKTNMDQVRTLEAGDDVIIQAFEETNQQQPFGTGRKLVREISVLRGNEKLAG
                     FGGLGQRPNPMTERGINTNSGSTTGGISGGVLPGAATGGSITTTVGEFTGAAPCWNCE
                     PQPGWGYQTVAPETKVQNKSDYGTEVTKPNLVKGLQ"
     gene            complement(3102..3188)
                     /locus_tag="VFR82_00710"
     tRNA            complement(3102..3188)
                     /locus_tag="VFR82_00710"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3152..3154),aa:Leu,seq:caa)
     gene            3323..3604
                     /locus_tag="VFR82_00715"
     CDS             3323..3604
                     /locus_tag="VFR82_00715"
                     /inference="COORDINATES: protein motif:HMM:NF016265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+)-trafficking protein"
                     /protein_id="HEU4500147.1"
                     /translation="MADVQCVTCGQTGESITDPLFMGKLETEIKAKVCKPCWKKWESM
                     RVMVINEYQVNLGDESGRELVKKQMKAFLKIGEEQADTSKLDQNYRPQS"
     gene            3763..4218
                     /locus_tag="VFR82_00720"
     CDS             3763..4218
                     /locus_tag="VFR82_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEU4500148.1"
                     /translation="MITQMHLKGLMFDPYNNAYIVVLRDEEGHEMLPIWVGKAEAGAI
                     SMALENVAPPRPMTHDFMKSVLDAYDAKVISVVITDLNENTYFAKIHLMYEDSEYTVD
                     ARPSDAIALALRSNAPVFAKETVLNKQSSEELGQWLENLKPEDFGKLDS"
     gene            4218..4715
                     /locus_tag="VFR82_00725"
     CDS             4218..4715
                     /locus_tag="VFR82_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEU4500149.1"
                     /translation="MEQPPSSPDRGLIAYKVDRVLDETNTDTKILVLSRQDGQSDTFP
                     IWIGAAEGHAIKLALDTTLTPRPMSHDLIKSIAEHLNVHVRRIVIGDVKNSTYYATVY
                     LENNGVERTVDARPSDAISLALRTNAPLYITEDVLARRNTANLDSWLAKQDKNSLNQQ
                     EVRES"
     gene            4758..>5157
                     /gene="rplM"
                     /locus_tag="VFR82_00730"
     CDS             4758..>5157
                     /gene="rplM"
                     /locus_tag="VFR82_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU4500150.1"
                     /translation="MSTYLQKPADVQEKWYLVDAEGKTLGRLAARVAGMLRGKHRPTF
                     TPNVDMGDHVVIINAEKVHLSGDKMETKLYRSHSGYPGGLKTATAKHVFRKDPTRLLV
                     EAIEGMLPKTPLGNHMAKKLRVYVGATHPHQ"
BASE COUNT         1247 a         1488 c         1400 g         1022 t
ORIGIN      
        1 ccctgattcc ctggtacgcg cacagtcggg tcagggcctc tccaaggcga ctttcgggaa
       61 cagccagttt ttcccggatt tccacgggtg cggttttatg gctgagtccg accacgacga
      121 tatgcatatc aggagactga cccgaaactt ttgagggcca cacccaccag cgtgagcacc
      181 actccggcga atccaataat cgtcaaatag gcggcgcgtt tggcacgcca tccgaccgtg
      241 agccgcccga caagtacgca aaagtaaaac agccaggtca ccaacgccca ggtctgttcc
      301 ggattccaat tcacgtaggt gccgcgtgca aattcggctg agattgcgcc ggtaatgatc
      361 ccaagcgtga gaagcggaaa acccaacaca atcgattgtt ggttcaaatc atcaagaaaa
      421 tccagggcgg gcagcttcga atacagcacg ttgaagcgtt tggacttgag aaggccatcc
      481 tgaatcagat acatgactcc cgcgacaaag gcgacggcaa agccgactgt gccgagcatg
      541 ctgagcgtca catgaaccca cagcgtacga aacaccggct gaagggtggg aaccgtttcg
      601 ggaagcgcag cggccgacac cagagagacg agcgccagcg gcacgatgaa cgatcccagc
      661 acatgaaggc ggtgacgaaa ttctacggcg agaaacacga ggatcaacat ccaggagaaa
      721 aacgacagcg cttcgtgaat gctcggtgtc gaggcccccg gccccgtcag catccgcata
      781 acaagcgcga cggtgtgtgt ggcaaaacca acggcagtaa tcgtgagtgc gaaggttgag
      841 agggcatcag cccgccgcag cagcagggca aggaaggaca ccgtggccgc aaaatacagc
      901 gccatagtga ccatgaaaaa cacggcggcc atgaagtcta tcccctgaac ttttcaggac
      961 aattggcctg ggcgagtgcg tagaattata tcgatggcgc gtcaaaagag tcaacaaacc
     1021 tctcgcaagg cgtccgattc tcccaacagt cgccgtcgtt ccggcacgct ctttatcgtc
     1081 ggaacgccta tcggatcgct ggacgatctc acgatccgtg cgcgagcggt cctcggagag
     1141 gtctccatcg tggcggcaga gaacccgctc gtcacgcaag cgctccttgc aaagcaccga
     1201 atcagcgcca ccattaccag ctatggacgc agagacgaaa acaagattgc cgtcctgctg
     1261 gaccggctca aagcgggcca cgatatcgcg ctcgtctctg actgcggcat gccggtcatc
     1321 tatgatcctg ggcgactgtt gattaccgct gcgcgcgatg ccggatatcc cgtcacggtc
     1381 gtgccggggc catcggctgt gaccgcggcc gccgcgttgt ctggatactc aggcgaccgc
     1441 ctcctcttcc tcggtcgcgt acctcggacg cgtccccacc tcgatcaatt gtgttcgaca
     1501 ctcaaacgtg aatcagcgac cgccatcatg ttcgtgcatc cacctatttt gccgcatatt
     1561 ctcaacagca tcaggcgcgt ccttccgaac cgagccgtca cgctggcggt caacatcacg
     1621 aaaaaggatg aacgggtgtg tcagggatcc gcgacatcgc tgcttgagat cgctcgatct
     1681 ctcccgcaag acgcggaact cacgctggtg ctcgctggcg tacggaaaag ataaaggcga
     1741 ctcagctgcg ccggataaat acgcgatagg attgttggag aggttcctgc gataacacct
     1801 cgtggccttc gttgctgacg ctcatcggga cgtttctgat cggatccccg gcatcgagaa
     1861 tcaccgaaag aatctgtcca ggctccatgg tttcaagctt caacttcgtt ttgacgaagt
     1921 tgtaggggca aatcacccct ctcaaatcca attcggcgtc aggttgaacc gcttgctcct
     1981 gctcgttcac atcgtctccg ataggcgttc agagaatcgt atcaaggaca tgaattgtcc
     2041 gcaatggaat tgaagaaccg gtcccgccgg cctgagacca ataaaaaggg gcagccgcaa
     2101 ggctgcccct tcgcaacgtt ttcgatgcaa acttggttat tgcagaccct tgaccaagtt
     2161 gggtttcgtc acctcggttc cgtagtctga tttgttctgc accttggtct cgggcgcgac
     2221 ggtctgatag ccccatcccg gctgcggctc gcagttccag caaggtgctg caccggtgaa
     2281 ctcgccaacc gtggtggtga tggatccacc agttgccgca ccggggagca caccaccgga
     2341 tatgccgccg gtggtcgaac cactattggt gttgatgccg cgctcggtca tcggattcgg
     2401 acgctgaccc aaaccaccga atcctgcgag cttttcgttc ccgcgcagca cgctgatttc
     2461 gcgcaccagc ttgcgacctg taccgaaggg ctgttgttgg ttcgtttcct cgaatgcttg
     2521 aataatgaca tcgtcaccgg cttccaatgt gcgaacttgg tccatgttcg tcttatcatc
     2581 gaaatacaca gtgcggtaat tcatcgcgcc gccgcccgca cggccttcat catgagagct
     2641 gcctccgctt tccagatgca acttccttcc gggaagatcc acgcccagca cccggccata
     2701 caccgtttca gcctgcgata accgctcaag aaagcttgtc cggtcaaata cattcacgcg
     2761 agtgctattt ccggacacgt caccgacacc ttcaccgacg cccataccgg attggttgct
     2821 ctgcaaccgc tcctgggcga ccgctacact cacggagccg acaaagagaa ctgccgctcc
     2881 tagcgccaac accttacgca tgtgctgcct ccttggtgag tagaaaatag aaagtaatac
     2941 ggacaacatg ttggtacgac ggatgaccca tgaattcgtt taattttcat gacactaggg
     3001 gccggactat aggccagtct aaaaaacaag tcaagagggt ttgaagggtg ggttaaagaa
     3061 gactgtcggt cttcccgtct ggttctcatg agccaccctg gtggtgcccc gagggagggt
     3121 cgaactccca ctctcttgcg agaaccggat tttgagtccg gcgcgtctgc cagttccgcc
     3181 atcggggcag agaagtgaag gggcgccact tctaacacga aggtttcaca agggtcaatc
     3241 gtaagctccc cccttttatt tggcggaaac ccgatgttac aattctgcgg aaacactacc
     3301 attacaggag gggtgaaacg ctatggcaga cgtgcagtgc gtaacctgcg gccagaccgg
     3361 ggaatcgatt accgatccct tgttcatggg caaacttgag acagagatta aagccaaggt
     3421 ctgcaaacct tgctggaaga aatgggaatc gatgcgggtg atggtgatca atgagtacca
     3481 ggtcaacctc ggcgatgaaa gcggacgaga gctcgtcaag aaacagatga aagcgttcct
     3541 caagattggc gaagaacaag cggacacctc caagttggat caaaattacc gtcctcaatc
     3601 ttgaccccgg tttttcgcaa ctctccgggt cgcaaagtcc ggcggtttct tcagcgaagt
     3661 cgccggcttc gttgacaggc gaaattcaaa aatgctaggg tgcacagttc ctttcgcggt
     3721 ctggagaatt tctccccaac gtccggtgga gcgtcgtctg ctgtgatcac ccagatgcat
     3781 ctcaaaggct tgatgttcga cccctacaat aatgcctaca tcgtcgtcct gcgcgacgaa
     3841 gagggccacg agatgcttcc aatctgggtc ggcaaggccg aagccggcgc catcagcatg
     3901 gccttggaaa acgtcgcccc tccccgcccg atgacccatg attttatgaa atcggttctc
     3961 gatgcctacg atgccaaggt catcagcgtg gtgatcacag acctcaacga aaatacctac
     4021 ttcgcgaaaa tccacttgat gtatgaagat tcggaataca ccgtggacgc gcgccccagc
     4081 gacgccattg cgctggcgct ccgatccaat gccccggtat tcgccaagga aaccgtgctc
     4141 aacaaacaaa gctcggaaga actcgggcag tggctggaga atttgaaacc ggaagatttc
     4201 gggaaattgg actcataatg gaacagccac cttcctcccc cgatcgggga ctcatcgcct
     4261 ataaagtgga tcgtgtcctg gatgaaacca acacagacac caaaatcctg gtgttgagcc
     4321 gtcaggacgg ccagtcagac acctttccga tttggatcgg tgcggccgaa gggcatgcga
     4381 tcaaactggc gctcgatacg accctgactc cacgtccgat gagccatgat ttgatcaaga
     4441 gcatcgcaga gcatttaaac gtccacgtgc gacgaatcgt catcggcgac gtgaagaaca
     4501 gcacctacta tgcgacggtg tatctcgaaa acaacggcgt ggaacgcaca gtggatgccc
     4561 ggccaagcga cgccatctcg ctcgccttgc gcaccaatgc accactgtac atcacggaag
     4621 acgtactggc gcgccggaac acggccaatc tggattcgtg gttggccaaa caggataaga
     4681 acagcctgaa ccaacaggaa gtacgagagt cttgatctgt caatgatgct atggcgatga
     4741 aggagcgcgg ggagacgatg tcgacttatt tacagaaacc agctgacgtg caagagaaat
     4801 ggtacttggt ggatgccgaa ggcaaaacgt tgggacgcct cgcggcccgc gtggcaggga
     4861 tgcttcgggg caagcatcgg ccgacgttca ccccgaacgt ggacatgggc gatcatgtgg
     4921 tcatcatcaa cgccgaaaag gtccatttgt ccggcgataa gatggaaacc aaactctatc
     4981 gctcgcatag cggctacccg ggcggcttaa agaccgcgac ggccaagcat gtcttccgaa
     5041 aagatccgac aagactgctc gtcgaggcga tcgaaggcat gctgccgaaa accccccttg
     5101 ggaaccacat ggccaagaaa ctcagggtct atgtgggcgc cacccatccg caccagg
//
LOCUS       DASTQY010000022        13243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1798331, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000022 DASTQY010000000
VERSION     DASTQY010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 13243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13243
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798331"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFR82_00735"
     CDS             <1..278
                     /locus_tag="VFR82_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500151.1"
                     /translation="YAVKGPWPSKSRAENLLFFAGEPVEHNTESDFAAKVAVLVERQR
                     LPSEAIALLSTRRAEPWDSGAVLTDDYAPYDLLIGTETGDDQPGGDR"
     gene            542..1021
                     /locus_tag="VFR82_00740"
     CDS             542..1021
                     /locus_tag="VFR82_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500152.1"
                     /translation="MGKTMLWVFFGLIMVIAGASSALAHQAGGVEVGDLETGSVVGQP
                     FKELDVDAELYAIEIGNLKTWYPPTTAIDFKVRAGRPVVIKVTNSSAAEHGFLLTAEE
                     TQSAPTVLKAQLVLKPGETKYVGIPTSDLFYATTGNVLKYRCHLHPAHVGGKLIMIK"
     gene            complement(1091..1420)
                     /locus_tag="VFR82_00745"
     CDS             complement(1091..1420)
                     /locus_tag="VFR82_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500153.1"
                     /translation="MRSRWILLGSLLWSLVCTWPSQASTGDIGSIVEKFVVRQFPDAA
                     SHYWVINQTQWDGDEMIVDVQAVVKERREAEPTLSRFLLLIVAGELKGSQNVPLEPAA
                     DCRTEEA"
     gene            complement(1619..1966)
                     /locus_tag="VFR82_00750"
     CDS             complement(1619..1966)
                     /locus_tag="VFR82_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU4500154.1"
                     /translation="MALLITDECISCGACLPECPNEAIFETRSDAETKGNHVGDGQGV
                     GDNIYVITHDRCTECVGHFDEPQCAAVCPVDNCCISDPAYPETTDVLLEKAKKLNPDK
                     AIDAAKVWSGVRN"
     gene            2168..2659
                     /locus_tag="VFR82_00755"
     CDS             2168..2659
                     /locus_tag="VFR82_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEU4500155.1"
                     /translation="MTVTSAQRDQKKKRIIIMILLAVLLLSVSMFLVPRDDAKTIIVT
                     FPNGTQIEVEVADTPEKLLFGLAFQEALPSNGGMIYIFESTGHHRVTTKAYRFPVDMI
                     WVDESRHIVSVMEHVQPCQQETCPWHGSTSDDVRYLIQTEAGFVQRQALTVGGELKYT
                     LHM"
     gene            2705..3028
                     /locus_tag="VFR82_00760"
     CDS             2705..3028
                     /locus_tag="VFR82_00760"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEU4500156.1"
                     /translation="MAIEGFQLVAKVEDLPAGQSKVVTLNNRTIALFNVEGKVYAIHN
                     LCPHEGGPLNEGRVKGFVVSCPWHDLAFDIRNGQGTDGGGYCVGSYEVRIEGGEIYVG
                     PRRKN"
     gene            3051..3377
                     /locus_tag="VFR82_00765"
     CDS             3051..3377
                     /locus_tag="VFR82_00765"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HEU4500157.1"
                     /translation="MDVTDRLQGDGKRVDAILDQPIEIHLWEDRTRGELWIPSYDSSA
                     LALLGDDYVRVAGNNAVDNGQRTFEFKAMKPGTFTILFEKRMGWKFTAEDRRVFHVTV
                     SPNGRA"
     gene            3374..4219
                     /gene="dat"
                     /locus_tag="VFR82_00770"
     CDS             3374..4219
                     /gene="dat"
                     /locus_tag="VFR82_00770"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR01121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HEU4500158.1"
                     /translation="MIVPETAFLNGRFLPLAEATISIEDRGFQFGDGVYEVIRTYRGH
                     PFRLDAHFARLDRSAQAIDLRQPYPPEQWAKYVTEGLRLAGFPEAKIYVQITRGTAPR
                     DHAYAPDLQPTVLMTIRELQPLNAAIQANGVAAMTMKDIRWGRCDIKSINLLANVLAR
                     QQARERGVFEAILVRDNQVTEGAVSNVFIVRDRKLITAPEGAWILSGVTREIVLGLAR
                     HEGLTVQERYCSEQELLGATEVFLTGTTVEVLGVVQIDGKQIGRGQPGPVTLSLAKRF
                     LDETR"
     gene            4341..4817
                     /gene="rimP"
                     /locus_tag="VFR82_00775"
     CDS             4341..4817
                     /gene="rimP"
                     /locus_tag="VFR82_00775"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU4500159.1"
                     /translation="MKESRPVVDRISEAVSPILWTLGLELADVVCVGQGSRSVVRVFI
                     DKPEGVTLEDCERAHKALGPALDVADPFPHAYTLEVSSPGLDRPFKRLQDYRRAIGKR
                     VTLKLREPLAGQWRLVGTLTDVADQTVTLAVDDRPSVRAVTLEQRLIAEAKRAVTW"
     gene            4849..6000
                     /gene="nusA"
                     /locus_tag="VFR82_00780"
     CDS             4849..6000
                     /gene="nusA"
                     /locus_tag="VFR82_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEU4500160.1"
                     /translation="MNRELISVIDEIGRQKGIDKTKVIGAIEAALQTAAKKRFGQAEN
                     IQVEIDSKTGEISVVSKKIIVESVSNPKAEISLKEARAYDSEAEIGDEIGSLIEMDEL
                     GRIAAQTAKQVIFQKVREAEWEAVQKEYSTRQGDLVNGIILGMERRNYLVDLGKTEAI
                     LPIQEQIPRETYRRGDRVKAMLLEVRRTPKDVQVILTRSHPQFVSKLFELEVPEVMEK
                     IVEIKAVVREPGDRTKIAVTSREKAVDPVGACVGIKGSRVQAVVRELRGEKIDIITWT
                     SDPRVFIAEALNPATIEKVGVDEEKKSALVVVADSQLSLAIGKNGQNVRLAARLTGWK
                     IDIISATEYEKEKAERDKEIKAALAEEAEAQRQQEEARQQEKAQQEAEG"
     gene            6014..8521
                     /gene="infB"
                     /locus_tag="VFR82_00785"
     CDS             6014..8521
                     /gene="infB"
                     /locus_tag="VFR82_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU4500161.1"
                     /translation="MRVYELAKQLGMENRDLIPELKRLGITVASHSSALEPEAVQKAL
                     EKLASKHKASVKGVGRPGESDAGRQKDADGAHPPAGAKAGVHPPATDDSKPDKRRILI
                     KRKRDEAPSEELTPLGHEPLVAHSPEAAAVTASPLHSQSESPVVQHEPHATGTTATTE
                     PPVAPKPVPTVTPETLTGKKKALEELQAEGLKDKLKKVRKPGRVREEDEVRVRDDAAR
                     WQDLRAIPVQRRDDRSKHVHHSTPAEITKPRKKSVKLTPGMTVKEFAEMIGQRPADIV
                     RKLMDMGQMLTFNQPMHVEAASMIAEEGGFKIEISVEKAGEELLEDLIQVQGEARPEP
                     RPPVVTIMGHVDHGKTSLLDAIRETKVAEGEAGGITQHIGAYTVTVHGKQVTFLDTPG
                     HEAFTAMRARGAKVTDIVILVVAADDGVMPQTVEAIHHAKAAGVPLIVAMNKIDKPSA
                     NPDRVRNALSEHGLISEAWGGDTIMVEVSAKQKTGLDQLLEMILLQAEVLELKADPHQ
                     LAKGTVVEAKLERGRGPVATVLVQSGTLHVGDVFVVGPFSGKVRALINHTGSKVQQAG
                     PSVPVEVIGLPGVPSAGDVFQAVKDERIAREIAEDRARKQRAAELAGAGKVSLDDLFA
                     KIQEGSVKELSLVIKADVQGSSEALAAAIEKLPTEVVKIRVIHNGVGGITESDVLLAA
                     ASNAIIIGFNIRPEPKASDLAEKEHVDVRLYTIIYNALNDIKAAMEGLLEPTLKERVL
                     GRAEVRQVFTVSKAGTIAGSYVIDGTITRASAGVRVIRDNVVVYQGRLGSLRRFKDDV
                     REVQQGYECGIAVENFSDIKSGDVIEAYAIDKIAAKL"
     gene            8668..8955
                     /locus_tag="VFR82_00790"
     CDS             8668..8955
                     /locus_tag="VFR82_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEU4500162.1"
                     /translation="MSVVVGLCTIELFLGDSQSLKDKRQILSSLKDRLRQKFNLSVAE
                     IDAQDLWQRGVLAFACVANDGRYVNQVLDQAQNMIKSNPVVEIVQSRVELL"
     gene            9009..9413
                     /gene="rbfA"
                     /locus_tag="VFR82_00795"
     CDS             9009..9413
                     /gene="rbfA"
                     /locus_tag="VFR82_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU4500163.1"
                     /translation="MAKTGYSRAERVADQIRMEVADILMRRIKDPRVRSVTVTDVELT
                     NDLRIARVFVTALGTEAETKDVFAGLSKASGFIRSELGRRLTLRYLPELVFHRDVSGP
                     RGDRVLRLLDEIQDKGDGHSAEHDDARSSQHG"
     gene            9364..10371
                     /gene="truB"
                     /locus_tag="VFR82_00800"
     CDS             9364..10371
                     /gene="truB"
                     /locus_tag="VFR82_00800"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU4500164.1"
                     /translation="MAIQLNTTTLVPVSTGNAMTAMVRECDVMDGVLVVHKEAGWTSH
                     DVVAKVRHLLGATKVGHAGTLDPAATGVLPVLIGKGTRIAEYLVPWDKEYRACLRLGE
                     TTDTQDATGTVLQRRMADDVTPAGVEEAIGRFRGEIQQIPPMYSAVKVRGVPLYRSAR
                     AGKTIAREARAVTIHELEVLTIEGCDVTLRVACSKGTYIRTLCADIGAALGVGGHLRA
                     LARTRVGPLKVDRALTMDVVIARHALGRLADDVWSLDEILGALPAFVVDHQSADRVRH
                     GVPVAYSSIVSPQVEEVRRIGRMPVRLHDQGGQLLAVGRAPQTQADRIAIEKVLVNQS
                     G"
     gene            10400..10669
                     /gene="rpsO"
                     /locus_tag="VFR82_00805"
     CDS             10400..10669
                     /gene="rpsO"
                     /locus_tag="VFR82_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU4500165.1"
                     /translation="MALLKAAKAELIKGYRQHDTDSGSPEVQIAVLTNRISYLTEHFK
                     LHKKDHHSRRGLLQLVGRRRRLLDYLRDIDDARYRAVIERLGIRK"
     gene            10856..12976
                     /gene="pnp"
                     /locus_tag="VFR82_00810"
     CDS             10856..12976
                     /gene="pnp"
                     /locus_tag="VFR82_00810"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU4500166.1"
                     /translation="MAHAVELDLAGRTLRLETGRVAKQADGSIWASYGDTVVLATAVA
                     SQNAKPGIDFLPLTVDYQEKAYAAGKIPGGYFKREGRPSEKEVLTSRLIDRPLRPLFP
                     EGYYFETQVIASVLSSDRTGSSDVIGITAASAALAVSNIPFLGPIAGVKIGRVNGQFI
                     VNPDLETLKGSELDLVVAGTSDAVMMVEAGANELPEATMLAAIELAHSEIKKIVAKIN
                     ELRVLSGNKPKRGIATEEIPSDLTAQVKVLVAQGIRDAIMIPNKTARQERLDLILRDA
                     IEKLKQPDDPNRERHVKIVFHGLEYTEVRNMILEKGSRADGRGPADIRPISSEVGVLP
                     RTHGSALFTRGETQSLAVVTLGTTDDEQRIDALEGEYTRTFMLHYNFPPFSVGEARPL
                     RSPGRREVGHGALAERALKPVIPNKDQFPYTLRIVSDILESNGSSSMATVCGGTLAMM
                     DAGVPIKEPVAGIAMGLIKEKDRVMILSDILGLEDHLGDMDFKVCGTKRGVTALQMDI
                     KIGGITPALMHQALEQAKAGRLHILSCMQKALEAPRTNMSAFAPRIYTMKVKQDKIRE
                     VIGPGGKTIRGIQADCGVKINIEDSGIVTIASVDGASLEKAKEMVNRIVEEVEIGKTY
                     LGTVRKIMDFGAFVEVLPGTDGLVHISQLAHHRVKSVADEVAEGDQILVKVLEIDRQG
                     KIRLSRKETMPAPTGAGTKDQTSG"
     gene            12982..>13243
                     /locus_tag="VFR82_00815"
     CDS             12982..>13243
                     /locus_tag="VFR82_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU4500167.1"
                     /translation="MYRKLVLDNGMRVVTERIPTLKSVTVGIWVNTGSRDEQPAQAGY
                     SHFIEHMFFKGTRSRSASEISREIDGLGGEMNAFTTRETTTFY"
BASE COUNT         3063 a         3435 c         4050 g         2695 t
ORIGIN      
        1 cctatgcggt caaaggacca tggccgtcca aatcacgtgc cgaaaatctt ttgttctttg
       61 ccggcgagcc cgtcgaacac aatacggaat ctgattttgc tgccaaggtt gctgtcctcg
      121 ttgaacgtca gcgtcttccc tcggaggcga tcgccttgtt gtcgacccgt cgggcggaac
      181 cctgggattc aggcgccgtg ttaaccgatg attatgcccc atatgatctg ctcatcggaa
      241 cggaaacagg tgatgatcag cctggaggcg atcgctagca agatattgat tttgaactag
      301 ttgtcttgtg gacaggcagg acaggatgac gggcgcgttg ccccgaactc gcgtcattaa
      361 cgatgttgct tccgttttcg aggctgtgct ataaggttgc ctcccaattt aagagtacca
      421 aagtgggcgt tgataacgcg gatcggatca acgggtgtcg gaagggtcaa ggctacctga
      481 tcgtggcggt agttatttac caacctaatc acatctcttc ttctttttaa ggaggtaggt
      541 catgggcaag acgatgttat gggtcttttt cggcctcata atggtcattg caggggcgtc
      601 ctcggcgctg gcgcaccaag ccggcggagt cgaagttggc gatttggaga ccggatcggt
      661 ggtcggtcag ccgttcaagg aattggatgt ggatgcagag ctctatgcca tcgagattgg
      721 caacctcaag acgtggtatc cacccaccac cgcaatcgac ttcaaagtgc gagccggtcg
      781 gcctgtggtc atcaaggtga cgaacagttc agcggcagag cacggtttct tgctaacggc
      841 agaggaaacg cagtcggctc ctacagtgtt gaaggctcag ttggtcctca agcccggtga
      901 gaccaaatat gtcggtatcc cgaccagcga ccttttttat gcgacaactg gaaacgttct
      961 gaaataccgc tgccatctgc accctgcaca cgtgggtgga aagctgatta tgattaagta
     1021 gtcgtcttcg aacgcgagag atgatcagcg cccccgctgc ctggacggtt gtgggggcgc
     1081 tggtgtttta tcacgcttct tctgtccggc agtctgcagc aggttcaagc gggacgtttt
     1141 ggcttccctt cagctccccg gcaacgatca agagaagaaa tcgactcaac gtcggctctg
     1201 cttcccgccg ttctttcacg acggcctgga cgtcgacaat catttcatct ccatcccatt
     1261 gcgtttgatt aatgacccag taatgactgg ccgcatcggg aaattgtcgg acgacaaatt
     1321 tttcgacgat gcttccgatg tctccggtac tggcttggga tggccaggtg cagaccaatg
     1381 accacaggag acttccgagc agaatccagc gagagcgcat agctcacctc gcgcgtgatg
     1441 gcagatcggc tccatgggaa ttaggtgggc gtgttaggag ccggatgtgg aatctaaata
     1501 gattgtactg cgagagcgga gacaatgcta cccaaagcgc aagactcgcc atgaaaacgc
     1561 acgagccctg cttctcagaa cagaagcagg gctcgcgtcc ggtcgctata gcggtaaatt
     1621 agttccgaac gccgctccag accttggcgg catcgatggc cttatcggga ttcagtttct
     1681 tggccttctc aagtagaacg tccgtcgtct cgggataggc gggatcagaa atgcagcaat
     1741 tatccactgg acatacagcg gcgcattgtg gttcgtcaaa atggccgacg cactcggtgc
     1801 agcggtcatg ggtgatgaca tagatgttat cgcccactcc ttgcccgtcg ccgacatggt
     1861 tgcccttcgt ttccgcatcg ctgcgagttt caaagatcgc ttcgttcgga cattcgggta
     1921 ggcaggctcc acaggagata cattcgtcgg taatcagcag tgccatgacc ctcgcctcct
     1981 tctcactaca gctacaaacc aggttgacaa gattgcgacg ccacgacata gtgcgtgacg
     2041 ttaataaggc gtcattctag tctgcggctc ttttccaagt caacagaacg gcgtctgtgc
     2101 agaaggccta gatcccctgt tattatgggc ctttgggccc agatttgcgt ttgccggaaa
     2161 gagatctatg acagtgacct ctgcccaacg ggatcagaag aaaaagcgga tcattataat
     2221 gatcctgctt gccgtgctgc tcctgagtgt ctccatgttc ctcgtcccgc gggacgacgc
     2281 gaaaaccatt attgtgacgt ttcccaatgg tacccagatc gaggtagaag tcgctgacac
     2341 gcccgagaag ttgcttttcg gattggcatt tcaagaagca ttgccctcga atggaggaat
     2401 gatctatatc tttgagtcta caggacacca tcgcgtgacc accaaagcct accgctttcc
     2461 cgtcgacatg atatgggtcg acgaaagtcg tcacatcgtc tccgtgatgg agcatgtcca
     2521 gccttgccag caggaaactt gtccatggca tgggtcgact tccgatgacg tgcggtacct
     2581 gattcaaact gaggcgggtt tcgtccagcg acaggccttg accgttggcg gcgagttgaa
     2641 atacacactg catatgtaag cgtcggatgt aagcgccgag ccaatccacg accggaggca
     2701 agttgtggcg attgaagggt ttcaactagt cgcgaaggtc gaagacctcc ccgcgggcca
     2761 atccaaagtt gtcacgctca acaaccgtac catcgccttg ttcaacgtgg aggggaaagt
     2821 ctatgccata cataacctct gtccgcacga gggcggtccc ttgaatgaag gccgagtgaa
     2881 ggggttcgtc gtctcatgcc cctggcatga cctcgcgttc gatatcagaa atggacaggg
     2941 caccgatgga ggcgggtatt gtgtcggcag ttatgaggtg cgtatcgagg gcggcgagat
     3001 ctatgtcggt cctcgccgga aaaactagca ccaccgacga aacggatgac atggacgtta
     3061 cagatagatt acagggagac ggcaagaggg ttgatgccat actcgatcaa ccgatcgaaa
     3121 ttcatctctg ggaagatcgg acgagagggg aactctggat tcccagctat gactcgtccg
     3181 ccttggcctt actcggtgac gactatgtca gggtggccgg taacaatgct gtcgataacg
     3241 gtcaacgaac ttttgaattc aaggcgatga aaccaggaac ctttacgata ctgtttgaaa
     3301 aacgcatggg atggaagttt acggcagagg atcggcgtgt atttcatgtc accgtttcgc
     3361 caaacgggag ggcatgattg tgcccgagac ggcgttcctc aatggacggt tccttccgct
     3421 ggccgaggcg acgatctcga tcgaagatcg tggctttcaa ttcggggatg gagtctacga
     3481 agtcatcagg acctaccgag ggcatccctt caggctggat gcgcatttcg ctcgcttgga
     3541 tcgcagtgcc caagccattg acctccggca gccatatcca cctgagcagt gggctaagta
     3601 tgtgacggaa ggtctccgtc tggctggttt tccggaggca aagatctatg tgcagattac
     3661 gagagggacg gcgccacgcg accatgccta tgcgccggat cttcaaccaa ccgtgctgat
     3721 gacgattcgt gagctgcagc ccctgaatgc ggccatccaa gccaacggcg ttgccgccat
     3781 gacaatgaaa gatatccgct ggggccgttg cgatatcaag agcattaatc ttctagccaa
     3841 cgtattggct cgccagcagg ccagggagcg cggcgtgttt gaggcaatcc tcgtacggga
     3901 taaccaggtg acggagggcg cagtcagcaa tgtttttatt gtgcgggacc ggaaactgat
     3961 caccgctccc gaaggcgcct ggattctctc aggagtgaca cgcgagatcg tgttaggatt
     4021 agccaggcac gaagggctca cggtgcagga gcggtactgt tcggagcaag agttgctcgg
     4081 tgccacggag gtgtttttaa ccggaacgac tgtcgaagtc ttaggcgtcg tgcaaattga
     4141 tggcaaacag atcggaaggg gacaaccggg ccctgtgaca ctgagtctgg ccaagcgttt
     4201 tctcgacgaa acgcgataat aggccccttg ctttgacaag ggtgaaatgg tattgtgcca
     4261 cctctgcaaa gtgggctcag gcccactttt ttgtttgaac gcgtacagaa ccatcaaggg
     4321 aaacggggct aggggtgttg gtgaaggagt cccgaccggt cgtcgatcgc atcagcgagg
     4381 cagtgtcccc catcctatgg acgttaggcc tcgaattggc ggatgtcgta tgtgtggggc
     4441 aagggtcccg ttcggtcgtg cgggtcttca tcgataaacc ggaaggggtc acgttggagg
     4501 attgcgagcg cgcacacaag gcgctcggtc cggcgctcga tgtggccgat ccctttcccc
     4561 atgcctatac gctggaagtc tcctctccgg gtttggaccg gcctttcaag cggcttcagg
     4621 actatcgtcg ggcgattgga aaacgagtca cgttgaagct ccgagaaccc cttgccggac
     4681 aatggcgcct ggtgggaaca ttgaccgacg tcgcggatca aacggtgacg ctggcggttg
     4741 acgatcgacc gtccgttcgt gctgtgacgc tggagcaacg gttgatcgcg gaggccaagc
     4801 gagccgtgac gtggtaagca ttgagggtgt tcgactggag accgaaacat gaaccgagaa
     4861 ttgatttcag tcatcgatga aatcggccgc cagaagggaa tcgacaagac aaaggtcatc
     4921 ggagcgatcg aggcggctct gcagactgcg gccaaaaagc gcttcggcca agcggaaaat
     4981 atccaggtcg aaattgactc gaagaccgga gaaatttccg tggtctccaa gaaaatcatt
     5041 gtcgagtcgg tcagtaatcc gaaagccgaa atctctctca aggaggctcg ggcctacgac
     5101 agcgaggcgg aaatcggaga cgagataggc tcgctgatcg aaatggatga gctcggacgc
     5161 atcgcggccc agacggccaa gcaagtcatt tttcagaagg tccgggaagc ggagtgggaa
     5221 gcggtccaga aagagtattc caccaggcag ggcgatctgg tgaatggcat catcctcggc
     5281 atggagcgtc gcaattatct ggtcgactta ggcaaaacgg aagccattct tcctattcaa
     5341 gagcaaattc cgcgtgaaac ctaccgccgt ggcgaccgtg tcaaagcgat gttattggag
     5401 gttcggcgga cgccgaaaga tgtgcaggtc attctgacac gcagtcatcc ccaattcgtc
     5461 tcgaagctgt tcgagttgga agtgcctgag gtcatggaaa agattgtaga gatcaaggcg
     5521 gtcgtacggg agcctggcga tcgcacgaag attgccgtca cctcccgcga aaaggctgtg
     5581 gatcctgtgg gcgcctgcgt gggaatcaaa ggctcccgtg ttcaggcggt tgtgcgcgag
     5641 ctgcgtggag agaaaatcga tatcatcacg tggacctcgg atccgcgcgt gtttatcgcc
     5701 gaagcgttaa accccgcaac aatcgaaaag gtcggagttg acgaggagaa aaaatccgct
     5761 ttggtcgtgg ttgcggactc ccaactgtca ttggcgatcg gcaagaacgg gcaaaatgtg
     5821 cggcttgcag cccgtttgac cggttggaaa atcgatatca tcagcgccac agagtacgaa
     5881 aaagagaagg cggagcggga taaagaaatc aaggcggcgc tcgcggaaga agcggaagcg
     5941 caacggcaac aggaagaagc acgtcaacag gaaaaggccc aacaagaggc ggaagggtag
     6001 caaatttact tgtatgcgcg tgtatgaact ggcgaaacag cttgggatgg agaaccggga
     6061 cttgattccc gagctcaagc gattgggaat taccgtcgct tctcacagca gcgccttgga
     6121 accggaggcg gttcagaaag cactcgaaaa gcttgcctcg aagcacaagg catcggtcaa
     6181 gggagtcggg cgacctggcg agtcggatgc gggacggcag aaggacgccg acggagccca
     6241 ccctccggca ggtgcgaaag caggagttca ccctccggcg accgacgact ccaagccgga
     6301 taagcgccgg atcctgatca aacgaaaacg agatgaggcg ccttccgagg aactcactcc
     6361 gctcggacac gaaccgcttg ttgcacactc acccgaagcc gctgcggtca ccgcctctcc
     6421 gcttcattcg cagtcggaaa gtccggtagt tcaacacgaa ccgcatgcta ccggcacgac
     6481 ggcaacgacc gagccgccag tggctcccaa gccggtgccg acggtgactc ccgagaccct
     6541 gacgggcaag aagaaagctc tcgaagagct ccaggctgag gggctaaagg acaagctcaa
     6601 gaaagtccgt aagccgggac gcgttcgcga agaggatgaa gtgcgcgtgc gcgatgatgc
     6661 ggctcgttgg caggatcttc gtgccatccc agttcagcgg cgtgatgatc gcagcaagca
     6721 cgtgcatcac agtacgccgg ccgaaataac caagcctcgc aaaaagagcg tcaaacttac
     6781 cccgggcatg accgtcaaag agtttgccga aatgattggc cagcgtccag ccgacatcgt
     6841 gcggaagttg atggatatgg gccagatgtt gacattcaat caaccaatgc acgtcgaggc
     6901 cgcttcaatg attgcagaag agggcggatt caaaatcgag atctcggttg agaaagcggg
     6961 agaggagctg ctcgaagatc tcattcaagt tcagggtgaa gcgcgtcccg agcctcggcc
     7021 gccggtagtg acgatcatgg ggcacgttga ccacgggaaa acgtcgctgc tggacgccat
     7081 tcgggaaacc aaggttgcgg aaggcgaggc cggggggatt acccagcaca tcggtgccta
     7141 cacggtcacg gtccacggaa aacaggtcac gtttctcgat acgccaggcc atgaagcgtt
     7201 cacggccatg cgtgctcgtg gagccaaggt caccgatatc gtcatcttgg tggtcgctgc
     7261 tgatgacggt gtcatgccgc aaaccgtgga agcgattcat cacgccaagg cggccggcgt
     7321 gccgctgatt gtagcgatga acaagatcga taagcccagt gccaacccgg accgggtacg
     7381 caatgctctt tccgagcacg ggttgatctc agaggcctgg ggcggcgata cgatcatggt
     7441 cgaggtgtca gccaaacaga aaacgggctt ggatcagctg ctggaaatga ttctgttgca
     7501 ggccgaagta ttggagttga aggcggaccc gcatcaactc gccaagggca ccgttgttga
     7561 agccaagctc gaacgggggc gtggaccagt cgccactgtc cttgtgcaaa gcggtacgct
     7621 gcacgtgggc gatgtattcg tcgtcggacc cttcagtggc aaagtccgtg cgctgatcaa
     7681 ccacaccggg tccaaggttc agcaggccgg gccatcggtt ccagtcgagg tgatcggttt
     7741 gccaggcgtg ccgtccgcag gtgacgtatt tcaagcggtc aaggacgaac gcatcgctcg
     7801 tgaaatcgcc gaagatcggg cgcgtaaaca acgggccgca gaattggccg gtgccggaaa
     7861 ggtgtcgctc gacgatctct tcgccaagat ccaggaaggg tccgtcaagg agttgtcgct
     7921 ggtcatcaag gcagacgtgc aagggtcttc ggaagcactg gccgccgcca ttgaaaagtt
     7981 gccgacagag gtggtgaaaa ttcgcgtgat ccataatggc gtaggcggga ttaccgaatc
     8041 cgatgtgctc ttggcggcgg cgtccaacgc catcattatc ggattcaaca tcaggcccga
     8101 accaaaagcg tcagatctcg cggagaaaga acacgtcgat gtccggctct acacgattat
     8161 ttacaatgcc ttgaacgata tcaaagcggc gatggaaggg ttgctggaac cgacgctcaa
     8221 agagcgcgtg ctcggccggg ccgaagttcg gcaggtgttc acggtgtcca aggccggcac
     8281 gattgccgga tcctatgtga tcgatgggac gattactcgg gcaagcgcgg gcgttcgggt
     8341 aattcgcgac aatgtcgtgg tgtatcaggg aaggctagga tcactccggc gtttcaagga
     8401 cgacgttcgg gaagtgcagc aaggctatga gtgcggaatc gctgtcgaga atttcagcga
     8461 catcaaaagt ggtgacgtca tcgaggccta cgccatcgat aagattgcgg caaagctgtg
     8521 accagcatgg tgaaaagttt tgtcggctgt gtgcccacgg tgtcgggagg cgtagcgctc
     8581 cacacgatct atgccccgcc gggccggcgt gacatcttgg acctaccgtc cgccagtgcg
     8641 ctgtcggcag tcctgacacg atagatcgtg agcgtcgttg tcggactgtg tacaatcgag
     8701 ctttttctcg gcgacagtca atcgctgaaa gataagcggc agatcctttc gagtctcaaa
     8761 gatcgactgc gacagaaatt caacctgtcg gtggctgaaa tcgacgctca ggatctgtgg
     8821 caaaggggag tgctcgcatt tgcttgtgtg gcgaacgacg gccgctatgt gaatcaagtg
     8881 ttggaccagg cgcagaacat gatcaaaagc aatccggtcg tcgagatcgt ccagtctcga
     8941 gtcgagttgt tgtgaggacg gtcgatgcct gtcagagcga cgatgggccg gccataagga
     9001 gctgggcgat ggccaaaaca ggatacagtc gtgccgagcg cgtcgccgat caaattcgca
     9061 tggaggtagc cgatatcctt atgcgaagaa tcaaggatcc gcgggtccgc tcagtgaccg
     9121 ttaccgacgt ggagcttacc aatgatctgc ggatcgcccg agtcttcgta acagcactgg
     9181 gaacggaggc ggaaacgaag gatgtcttcg ccggtctgag caaggccagc gggttcatcc
     9241 ggagcgagtt gggtaggcgg ttgaccttgc gatacttgcc cgagttggtc tttcacagag
     9301 atgtcagcgg cccacgcgga gatcgtgtgt tgcgattgtt ggacgaaatt caagacaagg
     9361 gtgatggcca ttcagctgaa cacgacgacg ctcgttccag tcagcacggg taacgcaatg
     9421 accgcgatgg tccgagagtg cgacgtgatg gacggcgtgc tggttgtgca taaagaggcc
     9481 ggatggacat cccacgacgt ggtggctaaa gtccggcatc tgttgggcgc taccaaggtc
     9541 ggtcacgcag gcacgctgga tcccgccgca acgggcgtac tacctgtatt gatcggaaaa
     9601 ggcacgcgca ttgctgagta cctcgtgccc tgggacaaag aatatcgtgc ttgtttgagg
     9661 cttggcgaaa cgaccgatac gcaggacgcc accggaacgg tgctgcaacg gcggatggcg
     9721 gacgatgtga cgccggccgg tgtcgaggaa gccatagggc gttttagagg tgaaatccaa
     9781 caaattcccc cgatgtattc agcggtgaaa gtccgcggcg taccgttgta tcggtccgcc
     9841 cgcgccggca agacgattgc ccgagaagcc agggcggtga cgattcatga attggaagtg
     9901 ctcacgatcg aaggttgtga cgtcaccctc cgtgtggcct gctcaaaggg gacctatatc
     9961 cgtacgctct gcgccgatat cggggccgca ttgggagtcg gggggcattt gcgcgccttg
    10021 gcgcgaaccc gcgttgggcc gctgaaagtg gatcgagccc tgaccatgga tgtcgtcatc
    10081 gcccgccatg cgctcggtcg actggcagac gatgtgtggt cgctcgatga gatcttggga
    10141 gcgctgccag cgtttgtcgt ggaccaccag tcggccgatc gggtgcgcca tggcgtcccg
    10201 gttgcctact cgagtatagt gtcgccccaa gtagaagagg tccggaggat tggaaggatg
    10261 cccgttcgtc tccatgatca aggcggccag ttactggcgg ttgggagggc acctcagacc
    10321 caggctgatc ggattgcaat tgagaaagtg ctcgtcaatc agtcaggatg atggacatgg
    10381 taaaaaggag agaattcaca tggcgttgtt aaaggcagcg aaagccgaat tgatcaaagg
    10441 gtaccggcag catgacaccg attccggatc tccggaggtg caaattgccg tgttgacgaa
    10501 ccgcatcagt tatctgacgg aacatttcaa gctccataag aaggatcatc attcgaggcg
    10561 tggattgctg cagctcgtcg gccgccgccg gagattgttg gactatcttc gggacatcga
    10621 tgacgcgcgg taccgggccg tcattgagcg attgggtatt cgcaagtagt caggaggccc
    10681 ctgctgtcgc gagcgtcgac agcaggcaac cgtcgcacag gtgaacactg acatgcgctc
    10741 ccgtgcaagt atgcgatgcg gaggttgagt gagccatagg cgatcaaccc atcgcacatc
    10801 aagaaggagc gcatttctgt gggcatctgt ggggcataac acgaggagac catagatggc
    10861 acatgcagtt gaactcgatc tggccgggcg cacgctacgg ctggaaaccg gccgggtcgc
    10921 caaacaggcg gacggctcga tctgggcatc atacggagat accgtggtcc tcgcgactgc
    10981 ggtcgcgtca cagaacgcca agcccggcat cgattttctc ccgctgaccg tcgattatca
    11041 agaaaaggcc tatgccgccg gaaaaatccc agggggttac ttcaaacggg aaggccgtcc
    11101 ctctgaaaag gaagtgctca ccagtcgatt gatcgaccgt ccccttcgcc cgctttttcc
    11161 tgaaggctac tacttcgaaa cgcaagtcat cgcatcggtg ctgtcgtctg atcggacagg
    11221 ctcctcggac gtcattggca ttaccgccgc atcggcggct ctggccgtgt ccaatattcc
    11281 cttcctcggt cctatcgccg gcgtgaaaat cggccgcgtc aacggtcaat tcatcgtcaa
    11341 tcccgatctt gaaacgttga agggaagcga attggatctc gttgtggccg ggacgtccga
    11401 cgccgtgatg atggtcgaag cgggggcgaa tgagttgccg gaagcgacga tgctggctgc
    11461 gatcgagttg gctcacagtg agatcaaaaa gattgtcgcg aagatcaacg agcttcgcgt
    11521 gctgtcgggt aataagccga aacgagggat cgccacggag gagattcctt ccgacttgac
    11581 ggctcaagtc aaggtgctgg ttgctcaagg aattcgtgat gcgatcatga ttcccaataa
    11641 gacggcgcga caggagcgcc tcgatctcat cctgagagac gccatagaaa aactgaagca
    11701 gccggatgat ccgaatcgtg aacgccatgt gaagatcgtg tttcatggcc tcgaatacac
    11761 agaagtgcgt aacatgattc tcgaaaaagg ctcgcgagca gacggccggg gaccggccga
    11821 tatccgtccc attagcagtg aagtcggcgt gctgccgcgc acgcatggat cggccctatt
    11881 tacccgaggt gaaacgcaaa gcttggcggt cgtgaccttg ggcactactg atgacgagca
    11941 acggattgat gcgttggagg gggagtacac acggacgttc atgctgcatt acaattttcc
    12001 gcccttcagt gtcggcgagg ccagaccgct ccgctcgcca ggccgccgag aagtcggtca
    12061 cggagcgctg gcggaacggg cgctcaagcc cgtcattccc aacaaggatc agtttccgta
    12121 tacgttgcgt atcgtgtcgg atattctcga gtcaaatggt tcctcgtcga tggcgacggt
    12181 ctgtggaggc acactggcaa tgatggatgc cggggtgccg atcaaagagc cggttgcagg
    12241 aatagccatg gggctgatca aggaaaaaga tcgggtcatg atcttatccg acatcctggg
    12301 gcttgaggat cacctcggcg atatggactt taaggtgtgt ggaactaaac gcggcgtgac
    12361 cgcccttcaa atggacatca agatcggtgg cattacgccg gccctgatgc atcaggcgtt
    12421 ggaacaggcg aaggccggcc gcttacacat tctcagctgc atgcaaaaag ctctcgaggc
    12481 gccgcgtacc aatatgtcgg cgtttgcgcc gcgcatttac accatgaagg taaagcagga
    12541 caaaattcgc gaagtcatcg gtccgggcgg aaaaaccatc cgcggcatcc aggctgattg
    12601 tggagtcaag atcaacatcg aggatagcgg catcgtcact attgcatcag ttgacggcgc
    12661 gtctctcgaa aaagccaaag agatggtcaa tcggatcgtc gaagaggtgg aaatcggcaa
    12721 gacgtacctg ggcacggtgc gaaagattat ggatttcggc gcgttcgtcg aagtgttgcc
    12781 gggaaccgac gggttggtgc acatctccca attggcccac cacagggtca aatcggtggc
    12841 ggacgaagtg gcggagggtg atcagatcct ggtgaaggtc ctggaaatcg atcgtcaggg
    12901 caagatccgc cttagtcgta aggaaacaat gccggctcca actggggccg ggacaaaaga
    12961 ccaaacctcc gggtaagcat cttgtaccgc aagctcgtcc tcgacaacgg catgcgggtg
    13021 gtgactgagc ggattccgac gctcaagtcc gtgaccgtgg gcatctgggt caacaccggg
    13081 tcgcgcgacg agcaacctgc ccaagccggc tactcccatt ttatcgaaca catgttcttc
    13141 aaagggacgc gctcgcgctc cgcaagcgaa atctcacggg agatcgacgg cttggggggc
    13201 gagatgaatg cgttcacgac gcgcgagacg acgacgtttt acg
//
LOCUS       DASTQY010000023         3628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_185519, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000023 DASTQY010000000
VERSION     DASTQY010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185519"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            402..1433
                     /locus_tag="VFR82_00820"
     CDS             402..1433
                     /locus_tag="VFR82_00820"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU4500168.1"
                     /translation="MEWSRTIRLVTLSVHVLAAVSMALATEPLKPEAQNPVAPAPSPT
                     PVPPKPHTQETDQKPSGKAIMEGTGKDPAAPSVPMPAKPPAKSASASNGVSPQKAPVI
                     MQPLPEIIGKDGAPMVLIPVGEFTMGSDKGDDDEEPVHKVFLDSFYLDKFEVTNGRFA
                     KFVEAIQSEPPWGFADKETPVLRQDQPVRWVNWMEAMGYCLWAGKRLPTEAEWEKAAR
                     GSDARVYPWGNDPPTLSHAVFGLKEGSDTVSAIGNRDKGKSPYGVHDLAGNLYEWTTD
                     WYDEQFYSKNPAINPRGPAEGSAKVQRGGSYINGAYRLRSSFRTKGDPTEHDPNVGFR
                     CAQDVPTQP"
     gene            1486..2304
                     /locus_tag="VFR82_00825"
     CDS             1486..2304
                     /locus_tag="VFR82_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEU4500169.1"
                     /translation="MKRYRVKPASRLSLKEHDPDDTGDYKKNDQGKAAAKAETEKHIA
                     CVRRLQERLYGNNSRALLIVLQGMDTSGKDSTIKQVMSGVNPQGCKVVAFKTPSTEEL
                     ARDFLWRVHREVPPRGFIGIFNRSHYEDVLITRVHGWVSKAVVKRRYDQIREFEDMLT
                     ENGTAILKFFLHISKDEQKIRLEERIHDPEKRWKWNSGDLEERERWADYMQAFEDVLS
                     ATSTEEAPWHIVPSNRKWYRNLVVADRIAHALEAMKLKTPPPPSGVDFAKLKIV"
     gene            complement(2903..>3628)
                     /locus_tag="VFR82_00830"
     CDS             complement(2903..>3628)
                     /locus_tag="VFR82_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU4500170.1"
                     /translation="FVIVIYCYLGWNVAGFIATEITDPRRTLPRVMIGGTAFVATIYV
                     LLNFVYLSALSIAGLAQEPVVPVAEKSATALWGPSSGHLVAALLCLSIAGAVSAMTWA
                     GPRVYWAMAQDGVLSSWLAHRHPESGVPARAILLQTLWASVLILSGTFEQLIVYSGLV
                     LAFFMCLTLSAIFPLRSRRDQPPGVYRVPGYPILPAILIISSLLVVCSSLLQRPIEAL
                     YGMVTVMAGIPFYLLWRRKRRLA"
BASE COUNT          855 a         1006 c         1024 g          743 t
ORIGIN      
        1 gttgtatctt ctttgttgat tctctcttgg gggactcggt ggagtctggg tgtgcagcga
       61 cggtaagttg agcctctgtt cggctcatcg gtgccagcgg taaagcgtaa agacagagta
      121 ccgcgagcgc gatcctgccg agccctgtgc tcctcaccat gttctcctgt agaggcccaa
      181 tacgtttgaa aggctaaggt attatgctga tccgcaatta caatcttcag caatacctca
      241 gaaggtgggt gctgagaaat ggatttatcg ccccggccgt tcacttgagt cggtgatgac
      301 acagatctga acagatttca ggggtgtggt ctagatttat agccgctctt ttgagtaccc
      361 tgaacgggtg cttaggaccc ttgcttgcag gatctccgcc tatggaatgg tcgagaacga
      421 ttcgcttagt caccctgtct gtacacgtgt tggccgccgt gtcgatggca ttggccactg
      481 agccactgaa gccggaggcg caaaatcctg ttgctccggc tccgagtccc actccagttc
      541 cgcccaaacc acatactcag gaaactgacc aaaaaccatc tggcaaagcg atcatggaag
      601 ggactggcaa agaccctgct gcaccgtcgg ttccaatgcc tgcgaagccg ccggccaaat
      661 ccgcttctgc gtccaatgga gtctctcccc agaaagctcc tgtcatcatg cagccgctcc
      721 cagaaattat aggaaaggac ggggcgccca tggtgctcat tccagtcggt gagttcacca
      781 tgggaagcga caaaggggac gatgatgagg agccggttca taaagtgttc ctcgacagct
      841 tttatctcga taagtttgaa gtgactaatg ggcgattcgc caagtttgtc gaagccatac
      901 aaagtgagcc cccctgggga tttgctgaca aggaaactcc tgtgctgcgt caggatcagc
      961 cggtccgctg ggtaaattgg atggaagcca tggggtattg tctgtgggcg ggcaagcgcc
     1021 ttcccactga agccgaatgg gaaaaagcgg cccgtggctc ggacgcaaga gtttatccct
     1081 ggggaaatga ccctcctacc ctctcccatg cggtctttgg tttgaaggaa ggttccgata
     1141 cggtctcggc catcggcaat cgtgacaaag gcaagagccc ttacggcgtg catgacctgg
     1201 ccggcaacct gtatgaatgg acgaccgatt ggtacgacga gcaattttac agcaagaatc
     1261 cagccattaa tccccgaggg cctgcagaag gatcagccaa agttcagcgt ggtgggtcat
     1321 atattaacgg cgcgtaccgc ctgcgttcgt ccttccgtac caagggtgat cccaccgaac
     1381 acgacccaaa cgtcggcttt cgctgcgcac aggacgtgcc aacgcagccg tagacatcgc
     1441 gaggctcgat tggccccgcg tcgcagtcca cttcctgaca agaccatgaa acggtatcgt
     1501 gtgaagccag ccagccgatt gtcgttgaaa gagcatgatc cagatgacac cggcgactac
     1561 aagaaaaatg accagggcaa agcggccgct aaggctgaaa cggagaagca tatcgcttgc
     1621 gtgcgtcgac ttcaggagcg actgtatggc aataacagtc gagcgctcct gatcgttctc
     1681 cagggtatgg acacaagcgg gaaagacagc accatcaagc aggtgatgtc cggcgtcaat
     1741 ccgcagggct gtaaggtcgt cgccttcaag actccctcaa cggaagaact ggcccgtgac
     1801 ttcctctggc gtgtgcatcg cgaggtgcct ccaagggggt tcattggaat tttcaaccgg
     1861 tctcattacg aagatgtgct cattactcgc gtgcatggct gggtgtccaa ggcggtcgtg
     1921 aagcgccgat acgatcagat cagagaattt gaagacatgc tgacagagaa cgggactgcg
     1981 atcctgaagt ttttcctcca tatttcgaaa gatgaacaga agatacggtt ggaggagcga
     2041 atccatgatc ccgaaaagcg atggaaatgg aattctggcg acctcgagga acgcgaacgc
     2101 tgggctgact acatgcaagc atttgaagat gtgctgtctg caacgagcac cgaggaagcg
     2161 ccttggcaca tcgtgccctc caatcgtaaa tggtatcgaa accttgtcgt ggccgatcga
     2221 atcgcccacg ccttggaagc catgaagctc aagactccac cacctccttc tggcgtagat
     2281 tttgccaagc tcaagatcgt ttaagaaaat ctacgaaatg cttttctcct ctacaatgct
     2341 ggcacgtgtt atgcgagtca ctggtcggtg ttgtgcccat tgacatgggt atctaatgag
     2401 cgtacgacgg atctgcgact tggacttaac tgagtcaccg aagattcggg agctcgggca
     2461 gaccgcgcgg ctgtcatctg gatctctcgg tgacttcaca agagaggagg gtggacatgg
     2521 acgacgcgac tccacctggc ccgtcaggcc cgccgatagg agggcgggtt tctcgtcgat
     2581 cgtgctagcc ggatcctcca ggctgtgccg tgatggcacc acagaccaac tggatattag
     2641 cctgattctc cggagcatcc gtcgcggcgc gaggaacaca agcgcctggg attgacgacg
     2701 tctcccacga cgagacactc gtatcacctg acatcgcgcg ggtcattcga accgcaacaa
     2761 tccaattgca atccggcgtc ctaagcgcca accacgggcg acctcccacc attgcaaggt
     2821 cgcccgtgcc atatttcgct cacacaatcc gcgtctcgtg cgcgcttctt ccgggcgagc
     2881 ccatcatttg ttctcggccc cctcacgcaa gtcgtctttt tctcctccac aataagtaaa
     2941 acgggatgcc tgccatcacc gtgaccatcc catacagagc ctcgatgggg cgctgcagca
     3001 aactcgagca cacgacgagt aacgaggaga tgatcagtat tgcaggcagg atcggatatc
     3061 ccgggacgcg gtacacaccg ggaggctggt cacgtctcga tcggaggggg aagatggcgg
     3121 agagcgtcag gcacatgaag aaggccaaga ccaggccgct atacacgatt aattgttcga
     3181 aggtaccgct gaggataagg actgaagccc agagtgtctg aagcagaatc gcacgcgcag
     3241 gcacgccgct ttctggatga cggtgggcca gccatgaact gagcaccccg tcctgcgcca
     3301 tcgcccaata gacgcgcgga ccggcccacg tcatggcgct caccgctccg gcaatggaga
     3361 gacagagcag tgcagcgacc aaatgtccgc tgcttggtcc ccacaatgcc gttgcagatt
     3421 tctccgcgac cggcacgacc ggttcctgag ccagcccggc gatggatagg gcagacaaat
     3481 acacgaaatt taagagcaca tagatcgtgg cgacgaaggc ggtgccgccg atcatgactc
     3541 tcggcagcgt gcgtcggggg tcggtgatct cagtcgcaat aaagcccgct acattccacc
     3601 cgaggtaaca atagatcacg atgacgaa
//
LOCUS       DASTQY010000024         8966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1795987, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000024 DASTQY010000000
VERSION     DASTQY010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8966
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795987"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1263)
                     /gene="clpX"
                     /locus_tag="VFR82_00835"
     CDS             complement(7..1263)
                     /gene="clpX"
                     /locus_tag="VFR82_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU4500171.1"
                     /translation="MAKQEKMDRHLRCSFCGKSRDEVRKLIAGPTVYICDECVNLCND
                     IIAEDWEEAKEEISSKLKKPLEIKHHLDQYVVGQERAKKILAVAVHNHYKRISAKEKD
                     VDDVELQKGNILMVGPTGTGKTLLAQTLAKYLDVPFTLADATTLTEAGYVGEDVENII
                     LKLLQASDYDVERAERGIVYIDEIDKISRKSDSPSITRDVSGEGVQQALLKLIEGTVA
                     NVPPQGGRKHPHQEFIQVNTSNILFICGGAFVGLEQIIEQRLNRKAMGFGAEIRGRAD
                     VRLGELFERVQPEDFLKYGLIPEFIGRLPVVATLDELDERALIKILTEPRNALTKQYE
                     KLLSFEKVKLKFTEGALGAVARKAFTQKTGARGLRAILEEVMLDVMYDAPSQKQIKEV
                     FITEDAILGKHPPIRIFEQDKDVRTA"
     gene            complement(1277..1906)
                     /gene="clpP"
                     /locus_tag="VFR82_00840"
     CDS             complement(1277..1906)
                     /gene="clpP"
                     /locus_tag="VFR82_00840"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEU4500172.1"
                     /translation="MLVPIVVEQTNRGERAYDIYSRLLKDRIIFLGAPIDDIFANLVI
                     AQLLFLEAEDPEKDINLYVNSPGGSVTAGLGIYDTMQYVKPPINTICLGQAASMGALL
                     LTAGTKGKRFALPNARVMIHQPLGGFQGQATEIDIHAREILKIRERLNEIMAKHTGQP
                     IEKIALDTERDYFMSGEEAKRYGLIDEVITRAPKALKAVASEKDGAKDK"
     gene            complement(1950..3263)
                     /gene="tig"
                     /locus_tag="VFR82_00845"
     CDS             complement(1950..3263)
                     /gene="tig"
                     /locus_tag="VFR82_00845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU4500173.1"
                     /translation="MKMEVTELGPMKRALKIEVPVEEVAQRFVLAYKELNRQVRVPGF
                     RPGKAPQALLEQRYAKAVEEDVVRSLVPDFYDRAVRQAGISPVVVEIPPLERVKIKRD
                     APFTFTATVEIKPKIELRDYKAPNPISLKPDTRTVTDEQLAKGMNVLREQHARLEAPP
                     AGHKAVEGDFVILTLEGFLDNQPLEGSKKTGHLHKVGSNATVLGLEIDRHVLGRAEGE
                     TVEISQPYPASHPDSRVAGKTVTFKVTVNAVKIKTLPELDDEFAKDCGAYSTLTELQE
                     RLRTQMEKALKREIEDTYKDTIVKRLVETHHFDLPETLVERELEAIIRQHMQRQRRTG
                     ADAPSALETEDLAKLRQENRDEAARRVKVGLILEAIAEKEQLTVTQEDINNEVQRLAT
                     ELKIAHTELVKMIQAGGQDSIEELRARILAEKSLDFVYRNAVIQG"
     gene            complement(3312..3394)
                     /locus_tag="VFR82_00850"
     tRNA            complement(3312..3394)
                     /locus_tag="VFR82_00850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3358..3360),aa:Leu,seq:tag)
     gene            3672..4754
                     /locus_tag="VFR82_00855"
     CDS             3672..4754
                     /locus_tag="VFR82_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU4500174.1"
                     /translation="MRIGLLLTSLAVLALTIGSSPVQAYRDYLTAEQKAQLEKIQTVL
                     VEAIALTDKGTVDAGPITEVAARRLGEMGYAVVRDAGKPHDATFKVKCEQRKTWEGTT
                     AAGGDADLPDAPSRLWKGPACQLSYLLGETKIKWQKEVRTEFEDAATAAQSANAGDPG
                     AYAMSQLRAKLEQYDFPVLLAAEWGHPDRLLKLLDASDTPQVRKIKIISLLGEMQADE
                     ALPQLKAALKDKNLSKEAAVALGSMGKDGIPILVDILKHSKQPELQAAAAKGLGDLGN
                     IHNDSSVVPPLLEMLDAPGVDIAVQTEVAWALGRVPDRRSVKPLFDLDKKLQKIRKDP
                     PDPQIKKLKEAVFWSIKQVYTEDQYS"
     gene            4934..6178
                     /locus_tag="VFR82_00860"
     CDS             4934..6178
                     /locus_tag="VFR82_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4500175.1"
                     /translation="MHDMSRRRFLQWTAVTGGALAFGGLMNRGTFMDFGGRTAYADEP
                     IKIGILDPLSSPYKTSSIHDVHGANVAVDLFNKKGGVLGRPVAILEADDASNPDQAVK
                     VATKFIKDDRVDVLMGTFNGDCALAVSALAAKENKLFMVTAAHAPELSGAACNSHTFV
                     FMPHARMLAHAVVPHLAKAYGTRWFMITASTIDGKSCAQALVDASQAHKVEIVGETVT
                     PFGTTDFGPAFTAAKEKQPTAVILNLYGWDLVNALKGYAKLELAKDKIGVGGLISGEQ
                     IGRPLGYAGNAGIWGLIWDPKINTDSSKRFIQGVIDKYNHTPTSRCYLGYAAMTQILE
                     AIQRAGSADTQALIKALEGHEFDGLKEGKSVFRASDHQHVQDVLVGEAYGKELGLGHY
                     KILSTMSGQANPGAADQQLCKH"
     gene            complement(6230..7369)
                     /locus_tag="VFR82_00865"
     CDS             complement(6230..7369)
                     /locus_tag="VFR82_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4500176.1"
                     /translation="MSTRFIRIVLALIAVSTVASCSGQKIETLKDAICCDGGYEYRHR
                     DRFAAEKDRQRLTDELAAARKENEALSARVRSVEDQLADRDRELASLRAKGEDSGRLS
                     SQLSSTQGDLDRANSRSSDLERQLADLHGGLADKDKLAAELLAAQAAVATLQAGSGDK
                     DKLAADLAQANKRVSELEAQLATLNAAGADKDKVAADLAAAQQRTAELERQVADRDRE
                     LAGLRGDLSTEMAKLKEAQRGLIRALRPQIEKGNIMVDLNNERLLINLASGYLFGSGE
                     DQLKSTGADALKHVGAILKDFPEYKVSVDGHTDNRPIRSELRKKFPSNKELSEARATN
                     AALALGEGGLTAVTTHGYADTRPIMPNTTDGGRAKNRRVEVRVTR"
     gene            complement(7415..7843)
                     /gene="mscL"
                     /locus_tag="VFR82_00870"
     CDS             complement(7415..7843)
                     /gene="mscL"
                     /locus_tag="VFR82_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU4500177.1"
                     /translation="MLKEFKEFAMKGNVLDMAIGVIIGAAFGKIVTSLVSDVLMPPIG
                     LLMGQVDFSSLFLNLSSTPQPSLAAAKAAGTPTINYGVFLQTVLDFIIIAFVIFMLVK
                     QINRLKKPAAPAAPTTKDCPLCLSTIPIKATRCAHCTSSV"
     gene            complement(7882..>8966)
                     /locus_tag="VFR82_00875"
     CDS             complement(7882..>8966)
                     /locus_tag="VFR82_00875"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEU4500178.1"
                     /translation="VRTVFLLVLVAGLWTEAAAEEPGPPAPAPVDMVTLKDGSVIFGE
                     VLEMDGGILLLKNSSNPDNLIKLKWDDVTKLTVTHPIPFHLKEGTILNGTATPGPDGV
                     LVIHAEPLKGTMDIPMSDISSVNPLIQPSVIYVGNLTGGYSQTTGNSHLRNASLIGEL
                     VARGDLLRLTMNVRYVYGDDSGALITRNAKGTIKLDFFITKRFFWFTSAYFENDRFQD
                     LKLRTALASGPGYQWIDRGDYEGLSKDMTLYTEAGLAYFNEDFNVAGDQSSVRLRISV
                     KWNWPIYEDRITIYHFSELFPSVQNTSDVFLTMDNGVRFKIWQNFVSSFQLTTRYNSR
                     PAPGTGDTDNLYLITLGYALDTTRKR"
BASE COUNT         1847 a         2514 c         2491 g         2114 t
ORIGIN      
        1 aagggatcag gcggtcctga cgtccttgtc ttgttcaaaa atgcggatgg gcgggtgttt
       61 gccgaggatg gcatcttcgg tgatgaagac ctccttgatc tgcttttgtg acggcgcatc
      121 atacatcaca tccaacatga cttcttccaa aatggcccga agtccccgtg cccccgtttt
      181 ctgagtaaag gccttccgag cgacagcacc cagcgcccct tcagtaaact tgagcttgac
      241 cttctcgaac gataacaatt tttcgtactg cttggtgagg gcgttgcgcg gttcggtgag
      301 aatcttaatc agcgcgcgtt cgtcgagttc gtcgagcgtg gcgacaacgg gcaatcgtcc
      361 gatgaattca ggaatgagcc cgtactttag aaaatcctcc ggttgcacac gctcgaataa
      421 ttctcccagc cgcacatcgg cgcgccctcg gatttctgcg ccaaatccca tcgctttgcg
      481 attcaaacgc tgctcgatga tttgttccaa tcctacaaag gctccgccgc aaatgaacaa
      541 gatattactc gtattgacct gaatgaactc ttgatgagga tgtttgcgtc cgccttgcgg
      601 cggcacattc gcgacggtgc cctcgatcag tttcaacagg gcctgttgga ctccttcccc
      661 ggatacgtcg cgcgtaatcg aaggactgtc gctcttccgg ctgatcttgt cgatctcatc
      721 gatgtacacg atgccccgct cggcacgttc gacgtcgtaa tcggaggcct ggagaagctt
      781 gaggatgata ttttcaacgt cctccccgac gtatcccgct tcggtgagcg tcgtcgcatc
      841 ggcaagcgtg aaaggcacat ccaagtactt ggccagggtt tgggcgagca gtgtcttccc
      901 tgtccccgtc gggcccacca tgaggatgtt gcctttttgg agttcgacgt cgtcgacgtc
      961 cttttccttc gcagaaatac gcttgtaatg attgtggacg gcgacggcca ggattttctt
     1021 ggctcgttcc tgtcctacga cgtactgatc aaggtgatgc ttgatttcta acggcttctt
     1081 cagcttcgag gaaatttctt ctttcgcctc ttcccaatcc tccgcaatga tgtcattgca
     1141 caagttgaca cattcgtcgc agatgtagac ggtaggtccg gcaatcagtt tacggacttc
     1201 gtcgcggctt ttgccgcaga aggaacagcg aaggtgtcga tccatctttt cctgcttggc
     1261 catgcatcct cccgtgttac ttgtctttgg ccccgtcctt ctctgacgcc accgctttca
     1321 atgctttggg cgctcgcgtg atcacttcat cgatcaagcc gtaccgcttg gcctcttctc
     1381 ccgacataaa gtagtcccgt tccgtatcca gcgcaatttt ttcgatgggt tgcccggtat
     1441 gcttggccat aatttcgttc agccgctcgc ggattttcag tatctcgcgt gcatgaatgt
     1501 cgatctccgt cgcttggccc tgaaatccgc ctaagggctg atggatcatc acccgggcat
     1561 tgggaagggc gaagcgtttg cctttggtgc ctgccgtaag gagcagcgcc cccatgctgg
     1621 cagcctggcc caagcagatc gtgttgatgg gcggtttcac atattgcatc gtatcgtaga
     1681 tgcccagacc ggcggtgacg ctgccacccg gcgaattgac atacagattg atgtcttttt
     1741 caggatcttc cgcttcaagg aacaggagtt gtgcgatgac gaggttggcg aagatatcgt
     1801 cgatcggagc gcccagaaaa atgatgcggt ctttcaagag ccgagaatag atatcgtagg
     1861 cgcgttctcc ccgattggtt tgctcaacga caatgggaac taacatgcag attcttcctt
     1921 tccgcgaaaa tgacagtgag acaggatggc tacccttgaa tcaccgcgtt tcgatacacg
     1981 aaatccaaag acttttctgc caggatgcgt gcccgcaatt cttcgatgga atcctggccg
     2041 cccgcttgga tcatcttgac gagttccgtg tgggcgattt tcaattccgt cgctagacgt
     2101 tggacttcgt tattgatgtc ttcctgcgtt actgtgagct gttccttttc agcgatggcc
     2161 tcgagaatca acccgacctt cacacggcgg gccgcctcat cgcgattctc ctgtcgcagt
     2221 ttcgccaaat cttctgtttc cagcgccgac ggggcgtccg cccccgtccg acgctgacgt
     2281 tgcatgtgtt gacgaataat tgcttccagt tcccgctcta ccagcgtctc cggaaggtca
     2341 aagtgatgtg tctcgacgag gcgctttacg atggtgtcct tgtaggtatc ttcaatctcc
     2401 cgcttgagcg ccttttccat ctgcgtccgc aagcgctctt gaagttcggt cagcgtgctg
     2461 taggcgccgc aatctttggc gaactcatcg tctaactcag gaagcgtctt gatcttgacg
     2521 gcgttaacgg tcactttgaa ggtgacggtt ttacccgcca cacgagaatc gggatggctt
     2581 gcgggatagg gctgcgaaat ttcaaccgtt tctccctccg cccttccgag aacatgccga
     2641 tcgatttcca atcctaacac ggtcgcgttg gaaccgactt tgtggagatg gcccgtcttc
     2701 ttgctgccct cgagcggctg attgtccagg aaaccttcca gcgtgaggat gacgaaatcc
     2761 ccctccactg ccttgtgtcc cgcgggcggt gcttccagcc tcgcatgctg ctcgcgaagg
     2821 acgttcatgc ctttggccag ctgttcatcg gtgacggtcc tggtgtcagg cttgagcgag
     2881 attggattcg gcgccttata gtcccgcagt tcgatttttg gcttgatttc gaccgtcgca
     2941 gtaaaagtga acggtgcatc tcgtttgatt ttgacacgct cgagcggagg aatctcgacg
     3001 acaaccggag agatacccgc ctgtcgaacg gcacggtcgt agaaatccgg caccaggcta
     3061 cgaacgacat cctcctcgac cgctttggca tagcgctgct ctagcaacgc ttgcggcgct
     3121 ttgcccggtc gaaaaccggg cacgcgaacc tgccggttca actccttata cgccaaaaca
     3181 aaccgctgcg cgacctcttc caccggcacc tcgattttga gggcccgttt catgggacca
     3241 agttccgtga cttccatttt catctgtcga gtgatttcct ttgcagtttt tatggcatgc
     3301 ccgctgccgg atggtgcgag aggggggact tgaaccccca cggttgccca cgagatccta
     3361 agtctcgcgc gtctgccagt tccgccactc tcgcacggtg gggaccgtcg gatggggtgt
     3421 ccagcctgta caaactagcg caactatacg gcgatgctgc tattttgtac cgttgcatgt
     3481 gtccaactgt caacccatac tccagcgggg attcaagagg attggctgat ttttgtctct
     3541 tgagggtaac agttggcacg gctagtgcac acaccaaaga ttctgaaagg tagcccgggg
     3601 tgtgcctgac cagtataatg gatcatcatg tgtcgtctca tctagtgtca gtacaagcag
     3661 ggaggtgcct catgcgcatc ggcttgctgc tcaccagtct cgctgttcta gcgctgacca
     3721 ttggttcttc tcccgtccag gcataccgcg attacctcac tgccgaacaa aaggcgcaat
     3781 tagaaaagat ccaaaccgtg ctggtcgagg cgattgcctt aacagacaag ggtactgttg
     3841 atgccggtcc gatcaccgaa gttgccgccc gtcgattggg tgaaatgggt tacgccgtgg
     3901 tgcgagatgc gggcaaacca cacgatgcga cctttaaggt gaaatgcgag cagcgcaaaa
     3961 cctgggaagg tacgaccgct gcgggcggcg acgcagacct tccggatgcc ccatcgaggc
     4021 tgtggaaagg gcctgcctgc cagttgagtt atctcctcgg ggagaccaag atcaagtggc
     4081 aaaaggaagt gcggacggag ttcgaagacg cagccacggc tgctcaatcc gccaatgccg
     4141 gcgatccggg agcctatgcg atgagtcaat tgcgcgccaa gcttgaacaa tatgatttcc
     4201 ccgtcctctt ggctgccgag tggggtcacc ccgatcgact actgaaattg ctcgatgcct
     4261 ccgacacgcc tcaggtgcgc aagatcaaaa ttatttcact actgggggaa atgcaggcgg
     4321 acgaggcatt accccaactc aaggcagcac tcaaagacaa aaatctttcc aaggaggcag
     4381 cagtagccct gggcagcatg ggcaaagacg gaattcccat ccttgtcgac attctcaagc
     4441 actccaagca gccggaactg caggccgcgg ccgcaaaagg tctgggagac ttggggaaca
     4501 tccataacga ctcgtcggtc gtgcctccac tcttggagat gctggatgca ccgggtgttg
     4561 atattgccgt tcaaaccgag gtcgcctggg cgttgggaag agttcccgac cgccgatccg
     4621 tgaagccgtt atttgatctg gacaaaaaac tgcaaaaaat ccggaaggac ccgcccgacc
     4681 cccagatcaa gaaactcaaa gaagcggtgt tctggtccat caaacaagtc tatacggaag
     4741 accaatacag ttagacgccg aaaccgttcc gctccggcgt cttttttctc tccgcgctgc
     4801 ctactcttgt tgttttgctg atcagaagaa atgcgtgtcg gttcgagcga ctacgcttga
     4861 gacaaggacg ggaactatga aatcatgggt ccggacagtt cttacgaaca tcacctgaga
     4921 ggaggccggc atcatgcatg atatgtcacg caggcgcttc ttgcagtgga cggcggttac
     4981 cggcggcgcg ctggcgtttg gaggcctgat gaaccgcggc acattcatgg acttcggcgg
     5041 gcggacggcg tatgccgatg agccgatcaa gatcggcatt ttggatcccc tgtcgagtcc
     5101 ttataagacc tcctccatcc acgacgttca cggggccaac gtggcggttg atttgttcaa
     5161 taagaaaggc ggtgtgctgg gacgcccagt ggccattctc gaggccgacg atgcgtccaa
     5221 ccccgatcaa gccgtcaaag tggcgacgaa gtttattaag gacgaccgcg tcgatgtgtt
     5281 gatgggcacg tttaacggcg actgcgcgct cgcagtgagt gcgttggcgg ccaaagaaaa
     5341 caagcttttc atggtaacgg ctgcgcacgc cccggaattg agtggagcgg cctgcaattc
     5401 ccatacgttc gttttcatgc cacatgctcg catgctggcc catgcggtcg tgccccattt
     5461 agccaaggcg tacggcaccc gctggtttat gattacggcg tcgaccatcg atggaaaatc
     5521 ctgtgctcag gcgctcgtcg atgcgtcgca ggcccacaag gtcgagattg tcggagagac
     5581 ggtgactcct ttcgggacga ccgattttgg acctgcattc actgccgcaa aagagaagca
     5641 gccaaccgca gtaatcctga atctgtatgg ctgggatctc gtcaatgcgc tcaagggcta
     5701 tgccaaactt gaactggcta aggacaagat cggcgttggt ggactcatca gcggcgaaca
     5761 gatcggacgc ccgctgggct atgctggcaa cgcggggatc tgggggttga tctgggaccc
     5821 caagatcaat acggacagtt ccaagcgttt tattcagggc gtgatagaca agtacaatca
     5881 cacgccgacg tcacggtgct atctgggcta tgctgcgatg acacaaatcc tggaagccat
     5941 tcagcgcgcc ggcagtgcgg atacgcaggc attgatcaag gccctcgaag gccatgagtt
     6001 cgacggcttg aaagagggca agtcggtctt tcgtgcctca gatcatcaac acgtccagga
     6061 cgtgctcgtc ggggaggctt atgggaagga gctgggattg ggccattata agattctttc
     6121 gacgatgtca gggcaggcca accccggcgc ggccgatcaa cagctctgca aacactaaca
     6181 ctgaccggcg ccgggagcgc aaaatgcgcc ccggcgacca gccaaccact tacctggtca
     6241 cgcgaacttc gactcggcgg ttttttgctc gtcctccatc ggtcgtgttt ggcatgatcg
     6301 gtcgcgtatc ggcatagccg tgcgtcgtca cggcggtcaa cccgccttct cccaacgcca
     6361 gggcagcgtt ggttgcccgg gcctctgaga gttccttgtt cgacgggaat ttctttctga
     6421 gctcgcttct gatcggccga ttgtcggtat gaccgtccac gctgactttg tattctggaa
     6481 aatctttcag aatcgctccg acgtgtttga gggcgtcggc gccggtggat ttcagctgat
     6541 cttcaccgga tccgaacaga taccctgaag ccagattgat gagcaggcgc tcattattca
     6601 agtcgaccat gatgttgccc ttctcgatct gaggccgaag tgcacggatc agaccgcgct
     6661 gggcctcctt cagtttggcc atctccgtcg agagatctcc gcgaagtccg gccaattccc
     6721 gatcgcgatc ggcaacttgc ctctccaatt cagccgttcg ttgttgcgcg gccgccagat
     6781 ccgcagccac cttgtccttg tcggctccgg cggcattgag cgtcgcgagt tgggcctcga
     6841 gctccgaaac ccgtttgttg gcctgtgcaa gatccgcggc gagcttgtcc ttgtctccac
     6901 ttccagcttg aagagtcgca accgcagcct gggctgcgag cagctcggcg gcaagtttgt
     6961 ctttgtcggc cagtccgcca tgaaggtccg ccagttggcg ctcgaggtcg ctgctccgtg
     7021 aattcgcgcg atcaaggtct ccctgcgtgc tcgaaagctg gctgctcaag cgcccgctgt
     7081 cctctccctt cgcgcgcagg gaggccaatt cccggtcgcg atcggccagt tggtcttcga
     7141 ctgacctcac tctggccgag agggcctcgt tttctttcct ggcggccgcc aactcatcgg
     7201 tcaaccgttg ccgatctttc tcggccgcga accggtcgcg atggcgatat tcatacccgc
     7261 cgtcacagca tatggcgtcc ttcaaggtct cgattttctg accactacag ctggcgaccg
     7321 tgctgactgc gatcagagca agtacgattc ggatgaaacg cgtcgacata tgttctcctc
     7381 acggtgacga tgcaaaaatg ggcgcatcca ttcctcatac cgaagatgtg caatgcgcac
     7441 acctggtggc tttgatggga atcgtcgaca ggcacaacgg gcaatctttc gtggtcggag
     7501 ctgcgggagc cgccggcttc ttcagcctgt ttatttgttt gaccagcatg aagatcacaa
     7561 acgcgatgat gatgaaatcc aacaccgttt gcagaaacac accgtaattg atcgtcggag
     7621 ttcccgccgc cttggccgct gcgagtgagg gctgaggtgt gctcgacagg ttgagaaaga
     7681 ggctggagaa atcgacttgg cccatcaaga ggccgattgg cggcatcaac acgtcgctga
     7741 caagtgaggt aacgattttc ccgaaggcgg cgccgatgat cacaccgatc gccatatcga
     7801 ggacgttgcc tttcatggcg aactccttaa actctttcag catgggtgcc tcctggtagt
     7861 aggaaggtga acggcgattg tctagcgttt tctggtcgtg tccagcgcat aaccgagtgt
     7921 gatgagatac aggttgtcgg tgtcgccggt gcccggcgcc gggcgactgt tgtaacgagt
     7981 cgtcaattga aagctgctga cgaaattttg ccagatcttg aaacgcacgc cgttgtccat
     8041 cgtcaggaac acatcggatg tattctggac ggagggaaac aattcgctga aatgatagat
     8101 cgtgatgcgg tcttcgtaga tcggccagtt ccacttgacc gaaatccgca ggcggacact
     8161 cgactgatct ccggccacat tgaaatcttc attgaaatac gccaagccgg cttccgtgta
     8221 gagcgtcata tccttggaaa gaccctcgta gtctcctctg tcaatccact gatagccggg
     8281 gccgctggcc agcgcggtcc tcaatttgag gtcctggaat cgatcgtttt caaagtaggc
     8341 cgaggtgaac cagaaaaacc gcttcgtgat aaagaagtcc agcttgatcg tgcccttagc
     8401 gttcctggtg atgagtgctc ccgaatcgtc gccgtacaca tagcgaacgt tcatggtgag
     8461 ccgcagcaga tcgcctcgcg cgacgagttc gccaatgaga ctggcgttgc gcaaatgact
     8521 gttgcccgtg gtttgcgagt acccgcccgt aagatttccg acgtagatga ccgacggctg
     8581 aatcaaggga ttgaccgacg agatgtcgct catgggaatg tccatcgtac ctttgagcgg
     8641 ctctgcgtga atgacgagca ctccatcggg tcccggcgtt gcggtgccgt tgagaatcgt
     8701 gccttccttt aaatgaaatg gaattggatg ggtgacggta agctttgtca cgtcgtccca
     8761 tttgagcttg atcagattgt ccgggttgga cgagttcttc aaaaggagaa ttccgccgtc
     8821 catttcaagc acttctccga agatgacgct gccgtctttc agtgtgacca tgtcgaccgg
     8881 cgccggagcc ggtggcccgg gttcctcagc agcggcctct gtccagagcc ccgcaaccag
     8941 tacgagcaag aaaactgtac gaacga
//
LOCUS       DASTQY010000025         4247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_988063, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000025 DASTQY010000000
VERSION     DASTQY010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988063"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1530
                     /locus_tag="VFR82_00880"
     CDS             121..1530
                     /locus_tag="VFR82_00880"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU4500179.1"
                     /translation="MGIAGKILIVDDEEDALENCRRILNRVPYTCVSTSNPSQALALL
                     EREHPGLVLTDLRMPKMDGIEVLTAAKRADPNVQVVLVTAHATIETAVTSMRYGAFDY
                     ITKPFTSDELIQVVRRAFDAEGGDGGGTPRTAVSPPRTAVSTHDGPVQIVGSSGLMRA
                     VLTLADRVAPTDSNVLIYGESGTGKEMLARYIHQMSRRVERPFLPVDCVSLNDALLES
                     ELFGHEKGAFTGAHAAKPGLFEVTHGGTLFLDEVSGMSPNLQARLLRVLQERQVRRVG
                     GTRFTHVDVRVIGASNQDLEDLCRKGTFREDLYYRLNVIPVALPPLRSREGDILLLAN
                     EFVTRFVQRVQADPVSVPVIDPSAADLLMRYAWPGNVRELQNVMERAAVLVDGHTVMS
                     THLPDRLQSLADKDSAPSESGSFKQAKQQAVINFERNFLLELLKRNEGHMSRAAREAG
                     VDRKTIERMVKKHGLRGTF"
     gene            complement(1568..2083)
                     /locus_tag="VFR82_00885"
     CDS             complement(1568..2083)
                     /locus_tag="VFR82_00885"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU4500180.1"
                     /translation="MEFKKIDPRVTITTKFGEIKIRFYPEDAPRHIENFISLIKMEFY
                     NGTTFHRIVPGFVIQGGDPLSKEPDRLKHGSGGPGFWLNPELSDRPHKRGAISMAKTP
                     RDGSSTRDFNDNGSQFFICLADNSGLDRRYSIFGEVIRGMDVVDKIAAVPRDEYNNPL
                     EPITMTMTVKE"
     gene            complement(2191..3483)
                     /locus_tag="VFR82_00890"
     CDS             complement(2191..3483)
                     /locus_tag="VFR82_00890"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4500181.1"
                     /translation="MINPARTLPIHWLILFLLFCTSVVTYMDRVNISVAARQMMPALG
                     LTQQDMGWVFSAFVVGYALFQIPGGWLADRWGARAVLTAALIWWSVCTAWTALAASSS
                     LADAVGIVGALITVRFALGMGEAVALPSFNRAVANWVPADSRGLGIGIAIGGIGLGSA
                     VTPPVAAWVMVNWGWQTVFYLSAGLGIFIGILWWSLARDRPSEHPWTVSRDGSSTTSA
                     GKDGGHAVSWKSLVRNPAIWWLVLSYSCLGYVAYVYLSWFYLYLVNVRGFDVLRGGLY
                     ASAPFWAILIGCPLGGWVTDRLAAVYGVTRGRVAAGMSGMILAGCAILAGAMADSATA
                     AVVSLSLGAGCLYFAVGAYWASTTDYSKTHAGLLSGLMNTGANLGGAVSPSLTPWIAA
                     QWGWPVSLATAGGIALIGAALWLKIDPGKGLQGRSEKS"
     gene            3777..>4247
                     /locus_tag="VFR82_00895"
     CDS             3777..>4247
                     /locus_tag="VFR82_00895"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEU4500182.1"
                     /translation="MSPIRLVVALITLIVVGGVAFLWLSPSLTGEDYLKDFFLHQLEQ
                     NLGRKIEVHRIKLVIFPTIRLEMKDIIIYDRNAESIMLSAKKLDLVLRLVPLLRKQVV
                     GKRLLVEDPTFTIRRDRSGRWNFLDRDNPLPTEDDEALQMMARIFRIREATIVNG"
BASE COUNT          918 a         1236 c         1228 g          865 t
ORIGIN      
        1 ctatggaatc gtgtcggatc atcgcgggcg cattcaagca aaaaatggga agaccggggc
       61 gatctttaca gtggtactgc cgactgcggg cgcgtcggtc cgcacgtcgc gaggggcatc
      121 atgggaatcg caggcaaaat cctgattgtg gatgacgagg aagacgccct ggaaaattgc
      181 cggcgtatcc tgaaccgtgt gccctatacc tgtgtctcga cgtccaatcc tagccaagct
      241 ttggcgctgc tcgagcgtga acatcccggc cttgttctga ccgacctgcg catgccgaaa
      301 atggacggca tcgaagtgtt gaccgcggcc aagcgagccg atccgaacgt gcaggtggtg
      361 ctggtcaccg cgcatgccac gatcgagact gccgtcactt cgatgcgcta cggcgcgttc
      421 gactacatca cgaaaccgtt caccagcgac gaattgattc aggttgtgcg tcgcgcgttc
      481 gatgccgagg ggggcgacgg agggggaaca cctcggacgg cggtgtcgcc gccgcggacg
      541 gcagtctcca ctcacgatgg gcctgtgcaa atcgtcggca gcagtgggct catgagggcc
      601 gtattaacgc ttgcagaccg ggtggcgccc acggattcca acgtcctgat ctacggcgag
      661 agtggaaccg gaaaggagat gctggctcga tatatccacc agatgagccg ccgggtggag
      721 cggcctttcc tgcctgtgga ctgcgtctcc ttaaacgatg ccttgctgga gtctgaactg
      781 tttggtcatg aaaagggagc cttcaccgga gcacatgccg ccaaacccgg cctcttcgag
      841 gtgacgcatg ggggaacact atttttggac gaagtaagcg gtatgagtcc caacctccaa
      901 gctcggctgt tgcgagtctt gcaagaacgg caagtccgtc gagtgggtgg aacgcgattc
      961 acgcacgtcg acgtccgcgt gatcggagcc tcgaaccaag atctcgaaga cttgtgccgg
     1021 aaggggacat ttcgcgagga cttgtattat cgtctgaacg tcattccagt ggcacttcct
     1081 cctctccgtt ccagagaagg ggatatttta ttgttggcca acgaatttgt gacgcgtttc
     1141 gtgcagcggg ttcaagcaga tcccgtctcg gttcccgtca tcgatccttc ggcggccgat
     1201 ctactcatgc gctatgcctg gcctggcaac gtccgggagc tccaaaatgt catggagcgg
     1261 gcggcggtgc tggtcgacgg ccataccgtg atgagcaccc acttgcccga tcgattgcag
     1321 tctctggccg acaaagattc ggccccgtcg gaatccggat cgttcaagca ggccaaacag
     1381 caggccgtta taaactttga gcgcaatttt cttttagaac tgttgaaacg caacgaaggt
     1441 catatgagtc gggcagctcg tgaagccggc gttgatcgaa agacgatcga gcgcatggtc
     1501 aagaagcacg ggcttcgagg gacgttctaa ctcgcagtca tttgagtctc tcggtacatt
     1561 ctccgaacta ctctttgacc gtcatcgtca tcgtgatcgg ctcgagggga ttgttgtatt
     1621 cgtcacgcgg gacggcggcg atcttgtcca ccacgtccat tccgcgaatc acttcaccga
     1681 agatactgta ccttcgatcc aacccactgt tgtccgccag gcaaatgaaa aattgcgagc
     1741 cgttgtcgtt aaagtcgcgc gtgctcgagc cgtcccgcgg tgtttttgcc atcgaaatgg
     1801 ctccgcgttt gtgggggcgg tcacttagtt ctggattgag ccagaagccc ggtccaccgg
     1861 atccatgctt caggcggtcc ggttctttac tgagcggatc accgccttga atgacgaatc
     1921 ccggcacgat ccggtgaaag gtcgtaccgt tgtaaaactc catttttatg agactgatga
     1981 agttttcgat gtgacgcgga gcatcctccg gatagaagcg aatcttgatc tcgccgaact
     2041 tcgtcgtgat cgtcacacgg ggatcgattt ttttgaattc catcacaggg aatctaacag
     2101 gggtgcgctc cgggcggcaa gggggacata atggatggtg gccagcgtga tggcaatccc
     2161 gcactatgcg agcgcgttcg acgacgtggg tcacgatttt tcagatcgac cctgcaagcc
     2221 tttccctgga tcaatcttga gccaaagagc cgcgccgatc aacgcaatgc ctcccgctgt
     2281 ggcaagtgaa accggccagc cccactgtgc ggcgatccaa ggcgtgagac ttggagaaac
     2341 tgctccccca agattggcgc ccgtgttcat gaggcctgat aagagtcccg cgtgggtctt
     2401 agaatagtcc gtcgtcgacg cccaataggc ccccacggca aagtaaaggc agccagcccc
     2461 gagtgagaga ctcaccacgg cagcggttgc cgaatctgcc atggcgccgg ccaggatcgc
     2521 gcatcccgcg agaatcatcc ccgacatacc ggcggccacg cgccccctcg tcacgccata
     2581 tactgcggct aatcgatccg tcacccatcc tcccaatgga caaccgatca agatggccca
     2641 aaaaggtgcg gacgcataga gtccccccct cagcacatcg aatcctctca cgttgaccag
     2701 atacagataa aaccaggaga ggtagacgta ggcgacataa ccaaggcagc tgtagctcaa
     2761 gaccagccac caaatcgccg gatttcgtac gagcgatttc cagctcacgg cgtgcccgcc
     2821 gtccttccca gccgaggtcg ttgaggagcc atctcgactc acagtccacg gatgctcact
     2881 cggccggtcg cgagccagcg accaccagag gataccaatg aagatgccca gccccgccga
     2941 tagatagaac acggtttgcc agccccagtt caccatgacc caggcagcca ccggtggtgt
     3001 caccgccgag cccaagccga tgcctccgat cgcaatgccg attcccaaac cccgtgaatc
     3061 agcgggcacc caattggcga cggcccgatt gaatgagggc agtgcgacgg cttctcccat
     3121 gcccagggca aatcgcacgg taatgagcgc cccgacgatc cccaccgcgt cggctagcga
     3181 cgagcttgcg gcgagcgccg tccacgccgt gcagacggac caccagatca aggctgccgt
     3241 cagcacggct ctcgcacccc agcggtctgc cagccatcca cctgggattt gaaacaatgc
     3301 atatccgacg acaaacgcag agaacaccca gcccatgtcc tgctgcgtga gcccgagagc
     3361 cggcatcatc tggcgcgccg cgacagaaat gttgacccga tccatatagg tgacgacact
     3421 ggtgcagaac agtaagaaga gaatcagcca atggatggga agcgtccgag cgggattgat
     3481 cacgcgatgt tgaccgccga tggagagacg aacggtggcg gattatagcg ggtcttcccg
     3541 attgtcgcca ggagaaatgt atcagtagta gatgaaaatc tcctgttggt tcgcggactt
     3601 gctggctttc aggggggtga ttctgtaaga tgaccgcagg cttgttgcag tcgtgtcctc
     3661 tcgtccatcc gggatgttcc cgacacgtcg taagcgacgc gccggtcctg atcgttacat
     3721 gaagggccga gagcgggtgg ccagtgctga cctatcgtcc acagcggaga aactgagtgt
     3781 ccccgattcg tctcgttgtc gcccttatca cactgattgt ggtcggaggc gtcgcttttc
     3841 tctggttgtc gccctccctg acgggcgaag actatctcaa ggactttttc ctccatcagc
     3901 tcgagcagaa tcttggccgg aagatcgagg tgcatcgtat caagctggtc atctttccca
     3961 cgatccggtt ggagatgaaa gacatcatca tttatgaccg caacgccgag agcatcatgc
     4021 tctcggcgaa gaagctggat ctcgttttgc ggctcgttcc cttgctgcgt aaacaggttg
     4081 ttgggaaacg attgctcgtg gaagacccga cgttcacgat tcgacgtgac cgttcaggtc
     4141 gctggaactt cctcgatcgc gacaatccct tgccgaccga ggacgacgaa gcgctccaaa
     4201 tgatggcgcg gatcttccgc atccgggagg ccacgattgt gaacggt
//
LOCUS       DASTQY010000026         9736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1189550, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000026 DASTQY010000000
VERSION     DASTQY010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189550"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1721)
                     /gene="guaA"
                     /locus_tag="VFR82_00900"
     CDS             complement(165..1721)
                     /gene="guaA"
                     /locus_tag="VFR82_00900"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU4500183.1"
                     /translation="MELWHDRILVLDFGSQYTQLIARRIREAQVYSQILPCTVPLATI
                     LAYRPQGIVLSGGPSSVYEKKAPVVPKELFDQGIPILGICYGMQLVTHLSGGEVAKSK
                     RREYGRADLTIDDASDLFAGIGKDRSTVVWMSHGDRIERMPPGFRSIAHTENSPVAAM
                     KRVDGKRRVYCLQFHPEVAHTPEGTRLLRNFVYQICGSKPTWTMQSYVETAVKQIREQ
                     VGSERVICALSGGVDSSVAAALTHRAIGDQLTCIFVDNGVLRAGERDQVQKTFASQLH
                     LNLRMLDRTEEFLAGLKNVTDPERKRKNIGRLFIKNFEAEARKLKGVKYLVQGTLYPD
                     VIESVSFKGPSATIKTHHNVGGLPARMRLKLIEPLRELFKDEVRVLGKELGLPDEIIW
                     RQPFPGPGLAIRVLGSVTKERLAILRAAETIVDQEIRSAGLYRDIWQAFAVLLPIRTV
                     GVMGDQRTYENVVAIRAVTSLDGMTADWAKIPNEVLGRMSNRIINEVKGVNRVVYDIS
                     SKPPSTIEWE"
     gene            complement(1845..3311)
                     /gene="guaB"
                     /locus_tag="VFR82_00905"
     CDS             complement(1845..3311)
                     /gene="guaB"
                     /locus_tag="VFR82_00905"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU4500184.1"
                     /translation="MLEKEPRLGLTYDDVVLVPSKSAVLPSEVDVRTHLTRNIQINIP
                     IISSAMDTVTESRLAIAMAREGGVGIIHRVLTPTDQAAEVDRVKKSESGMILDPVTIS
                     PDQTIRDAHELMARYRISGIPVTKNKKLVGILTNRDLRFETRLDLKVSQVMKRDKLIT
                     APEGTSLEKAREILHEHRIEKLPVVNKQGDLKGLITIKDIEKRIKYPNACKDAHGRLR
                     VGAAVGVGPDTEDRLTQLKKAGVDLVVVDTAHGHAQAVLETVKMIKKQHPSLELIAGN
                     IATAEAAKDLLKAGVDAVKVGVGPGSICTTRIVSGSGMPQLTAIADCAKVLDGSGVPI
                     VADGGVKFSGDISKALAAGASAVMLGGLLAGTEESPGETVLYQARTYKVYRGMGSIGA
                     MERGGGDRYGQSGRPAQKLVPEGIEGRVPYKGPLSAVIYQLVGGVKSGMGYCGCKTIP
                     ELQKNASFIRQTVAGLRESHVHDVIITKEAPNYRMDWE"
     gene            complement(3333..6302)
                     /locus_tag="VFR82_00910"
     CDS             complement(3333..6302)
                     /locus_tag="VFR82_00910"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEU4500185.1"
                     /translation="MIKAWLLDEMFRFDRAPLTVEGTHGEWGAGDSIIEEEELPPAPA
                     NVAAKCGNGRITISWDPVPDALYYNLYFQTTKGVHIKFSELTRPIAGPEDFKTVIGVK
                     KEKATCLEGAQSPFIHDDLANGTCYHYVVTVVTAKGESLESQEVMAIPSPYLLAACIG
                     QEGVDDGEFSSPTGIALDKDGNLYVADTDNHSIQKFDKAGHFVARWGGEPSSQEGSFY
                     YPRGLAVGANDSLYIADSGNNRVQKFDLDGNVMQAWGKFGFAWRGADMGKFDVPWGLA
                     TDQDGNLYVSDTSNARIQKFKADGTPLLKWGRDGSFDGAFFFPRGVAVDFVGNIYVAD
                     EGNNRVQKFDARGSFLTKWGREGAGPGQFKAPWGIACDALGNVYVVDTGNHRIQKFDG
                     NGTFLCAWGNRGKSEGQLNFPYGIAVDKEGCVYVVDSGNNRILKYVPTDEEINRGKEE
                     RPKSQGTNATPPPSNVAVKAGDTETFLSWIEVPGAVSYNLYFNTSPLLTTQSGTKIEG
                     VTNPFTHTGLTNDTPYYYAVTAMFDGDVESELSRVVTATPVLIDITAPQNPYAVINHG
                     AFMTNSPEVVVTISANDIDTGVGAYYVSEAPMTPMAGTPGWVEVTPSLKFGATIPFIL
                     SPGDGQKTIFVWFKDVGGNVSTPASATILVNTSGYLCVSRWGRSGRGASLLHGGEFMA
                     PIYGLCIDQQGSLFVVDNGNNRIQKFDNAGNFIILWGNFGSANANFHNPTGIACDGKG
                     DVWVVDTNNHRVQKFDGKLGGYLMKFGSRGNGEGQFNSPWGIAVDRVRGYVYVVDSAN
                     FRVQKFDMTGEFIMSWGSFGNGDGQFYFPRGVAVDQSDGAVYVVDMGNHRVQKFDTST
                     NVLPQLLTKWGGSAEAGHASSPLSQEAGQLRSPWGIAVDGAGDVYVTDTGNHRIEKFD
                     KEGNFITQWGGYGNGDGQFNFPYGIAVDAKGSVFAVDSGNMRVQQFMPADEGSERLQE
                     EAEAAAEIEKAQQPQKA"
     gene            complement(6582..7370)
                     /gene="scpB"
                     /locus_tag="VFR82_00915"
     CDS             complement(6582..7370)
                     /gene="scpB"
                     /locus_tag="VFR82_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEU4500186.1"
                     /translation="MNLAELQDAATVEALDEGSAVDGNGVMGSTSPETGAAQIEPQEE
                     ALKGRELIGVVEALLFVSTEPVPVSRLATVIGTVSKSDVEQALKALEQELAQEGRGIQ
                     LVKLAGGYRLVTKSDYAPWLKRLDKAKSAQKLSRSALESLAIIAYKQPLVRGEIEEIR
                     GVETSGVLRTLLERKLVRIVGRKEVPGRPIMYGTTKFFLEHFGLQDITQLPPLREFKE
                     LGEAEQALLPMEANEVTLQEPPNSEQDLESWESQALIPEPVEQT"
     gene            complement(7373..8170)
                     /locus_tag="VFR82_00920"
     CDS             complement(7373..8170)
                     /locus_tag="VFR82_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU4500187.1"
                     /translation="MDNQLDGAEQAELPYQVRIENFEGPLDLLLHLIKKNEINIYDIP
                     IAMIAQQYLSYIEAMKELNLAVAGEFLVMAATLLQIKSKMLLPVEESAEDEDDGPDPR
                     EELVRRLLEYKTFKEAARQLDTQEKMWREIYSRPATPQKDAPVTDEAMLENIGLFDLV
                     DALQGILNRNPGKKLLEILPDNLTVRDRMNVILEALEGQESIGFEALFDASSHRLLII
                     VTFLALLELIRLRTVRVYQAESFGPILVSRSFSLVPDPAELDDSEWR"
     gene            complement(8228..9280)
                     /locus_tag="VFR82_00925"
     CDS             complement(8228..9280)
                     /locus_tag="VFR82_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEU4500188.1"
                     /translation="MNRPVKAVALLVVLLYSAGSAAAECAVESAAAGSQRLFKTHLSA
                     GCTPVEREARAVDASNLVAALKRGETIDLAGVVIRGDVALDALPMSENPPAVGGIIGD
                     GDTEIRVIAGPVAIVDSVVRGTVTHRAAEGALVFSGPVTFAGTTFERMVDLSRSVFTQ
                     PVALSGAVFLREGYFVQCHFLREVTGEKTAFGSHTRFHRSQFHGPVTLQQSGFSGMAE
                     FLEVRFDQDANLSRTYFKLGTGFSGSHFKQLADFSETLFDGDAFFTFTRFDGDAFFRR
                     ATFRATADFDDAQFNAREDFSKAFFEKGPQFTRAKRSAANPDSLGIENRQVQYVITLS
                     LLLFSAVLVAYLVRSR"
     gene            9375..>9736
                     /locus_tag="VFR82_00930"
     CDS             9375..>9736
                     /locus_tag="VFR82_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500189.1"
                     /translation="MHVRTLIAGTLLVGVFLQTAIASYAIDYLEVPVLAAVRYNTLGA
                     FAIMLMGWDKKSDPNAVQLQVYIAGVRYGKNHLGSMAQAFDYAIQRTPAVIHTGTVFV
                     QGVTDLPTTSDGPSAGAA"
BASE COUNT         2013 a         2938 c         2617 g         2168 t
ORIGIN      
        1 ccgcgcgagc gtgacgtata cccaacaccg ctcagaatga tctttccgag ctagctgttc
       61 cctcccacaa ggatcggcct ggatcagccc aaacgtgcct atctaaaagt attagtctcg
      121 tgtgccgcct tccgcgagca caatgactga ccaggctgat aactctattc ccattcgatg
      181 gtgctcggcg gtttcgagct aatatcgtag acgacgcggt tcacaccttt cacctcgttg
      241 atgattcgat tggacatccg gccgaggacc tcgttgggaa tcttcgccca gtcggccgtc
      301 attccgtcca ggctcgttac ggcgcgaatc gccacgacat tctcataggt tcgctgatca
      361 cccatcacgc cgactgtccg gatgggcagc agcaccgcaa acgcctgcca aatgtcgcgg
      421 tagagaccgg cgcttcggat ttcctggtct acgatcgtct ctgccgcacg taaaatggcc
      481 agccgttcct tcgtcacgga gccgagcact cgaatcgcca acccgggacc ggggaacggc
      541 tgacgccaga tgatctcatc cggcaagcct agctcttttc ccaacacgcg cacttcgtct
      601 ttaaacaatt cacgcagcgg ctcgatcaac ttgagcctca ttcgtgccgg cagtcctcca
      661 acgttgtgat gggtcttgat cgtcgcagac gggcctttga agctgacgct ctcaatgaca
      721 tccggataca aggttccctg gaccaaatac ttgacgccct tcagcttcct ggcctcggcc
      781 tcgaaattct tgatgaacag acgcccgatg ttcttgcgct ttcgctccgg atcagtcacg
      841 ttctttaagc ccgccagaaa ctcttcggtc cgatcgagca tccggagatt cagatgcagc
      901 tgtgaggcga aggtcttctg cacctgatcg cgctcgcctg ctcgtaggac gccgttgtcg
      961 acaaagatgc aggtgagttg atcgccgatc gcccggtggg tcagggccgc ggccactgat
     1021 gaatccacgc cgccgctcaa cgcacaaatg acacgctcac tgcccacctg ctctcggatc
     1081 tgtttgaccg ccgtttccac ataggactgc atggtccagg tcggtttgct gccgcaaatt
     1141 tgatacacaa agttccgcag taatcgggtg ccttccgggg tatgggcgac ttcaggatgg
     1201 aactgcaggc agtacacccg ccgtttgccg tcgactcgct tcatggcggc gaccggagaa
     1261 ttttccgtat gggcaatgga ccggaatccc ggcggcatcc gttcgatacg gtcgccatgg
     1321 gacatccaga cgaccgtgga gcggtccttg ccgatgccgg caaacaaatc actcgcatcg
     1381 tctatggtca ggtcagcccg accatattca cggcgcttcg acttcgcgac ttccccgccg
     1441 gaaagatgtg tcaccagctg catgccgtag cagatgccga gaatcggaat gccctgatcg
     1501 aacagttcct tcggaacgac cggagccttc ttttcgtaga cgctggacgg accgccggaa
     1561 agcacgatgc cttgaggtct ataggctaaa atcgtggcga gtggaacggt gcacggaagg
     1621 atctgcgaat aaacctgcgc ttcccgaatc cgtcgcgcga tcaactgggt gtactgagag
     1681 ccgaaatcga ggaccaggat tctatcgtgc cagagttcca tctttgacaa gagcgtatcg
     1741 tgtttatctg atggctgtta acaacagaaa agaaacggca ggcagagaag gcagcgttca
     1801 gcgaccgttc tatcggctag acgccagacg ccgttcgcgc ccgtttattc ccagtccatg
     1861 cgatagttcg gcgcttcttt ggtgatgatg acgtcgtgca catggctttc gcggagcccc
     1921 gcaacggtct ggcggatgaa cgacgcgttc ttctggagtt cgggaatcgt tttgcatccg
     1981 caatagccca tcccggactt caccccgccc accaattgat agatgacagc tgacagcgga
     2041 cccttgtagg gcacacgtcc ttcgatccct tcgggcacca gtttctgggc cgggcgcccg
     2101 ctctggccgt atcgatcgcc accgcctcgt tccatcgcgc cgatcgatcc catgccgcga
     2161 tagaccttgt aggttctcgc ctgatagagc accgtctctc cgggcgactc ctcggtgccg
     2221 gccagcaacc cgccgagcat caccgctgaa gcgccagccg cgagtgcttt gctgatgtcg
     2281 ccggaaaatt tgacgccacc atccgccacg atcggcacgc cactgccatc aagcaccttc
     2341 gcgcaatcgg caatcgccgt cagttgaggc attccggagc ccgagacaat gcgtgtcgtg
     2401 caaatcgagc ccggtccgac gccgaccttc acggcatcga ccccggcctt caggagatct
     2461 ttcgctgcct cggcggtggc gatgttcccg gcgattagtt cgagggacgg atgctgtttc
     2521 ttgatcatct tgactgtttc gagcaccgcc tgcgcatggc catgagccgt atccactacc
     2581 accaggtcca cgccggcttt cttcagttgg gtaagccgat cctcggtgtc cggcccgacg
     2641 cccaccgctg caccgacacg caaccgtcca tgggcatctt tacaggcgtt ggggtatttg
     2701 atccgctttt cgatatcttt aatggtaatg agtcccttga ggtcaccttg cttgttgacg
     2761 accggcaact tctcgatccg gtgttcgtga agaatctccc gggccttttc taggctggtc
     2821 ccttcaggcg ccgtgatcag tttgtcgcgt ttcatgacct ggcttacttt aagatccaat
     2881 cgcgtctcga accggagatc gcgatttgtg agaatcccga ccagtttctt gttcttggtc
     2941 accgggatac ccgaaatccg gtatctcgcc atgagttcgt gggcgtcacg gatcgtttga
     3001 tccggtgaga tggtcaccgg gtccaggatc atgccgctct cggatttctt cacccggtcg
     3061 acttccgccg cttggtcggt cggcgtcagc acgcgatgaa tgattcccac cccgccttct
     3121 cgcgccatag caatggccag tcgtgactcg gtgaccgtgt ccatcgcgga actaataatg
     3181 ggaatattga tctgaatgtt gcgcgtgagg tgggtacgaa cgtcgacttc gctgggaaga
     3241 acagcggact tcgagggcac gagaaccacg tcatcatagg tgagtccgag gcgtggttct
     3301 ttctcgagca tggtcctccc gttctccggc cgtcaggcct tctgtggttg ttgagccttt
     3361 tcgatttcgg ccgccgcttc cgcttcttcc tggagccgct cgctcccttc gtcggccggc
     3421 atgaactgct gcacccgcat gttgccgctg tcgacggcaa agacgctgcc tttagcatcg
     3481 accgcaatcc cgtatgggaa attgaattga ccgtccccgt tcccgtagcc gccccattgc
     3541 gtgatgaaat tgccttcctt atcgaatttc tcgatgcgat ggttgccggt atccgttaca
     3601 tagacgtccc ccgcaccgtc gacggcaata ccccacggtg accgcaattg accggcctct
     3661 tgcgacaagg gactgctggc atgccccgcc tcggcgctgc cgccccattt cgtcaataac
     3721 tgcggcaaca cgttcgtgct ggtatcgaac ttctgaacgc ggtggttgcc catatcgacc
     3781 acgtagacgg ccccatcgga ttgatcgacg gcaacgccac gggggaaata gaattgcccg
     3841 tcaccattgc cgaagctgcc ccaggacatg atgaactctc cagtcatgtc gaacttctgg
     3901 acacggaagt tggcgctgtc gacgacgtag acgtacccgc gtacgcggtc tacggcaata
     3961 ccccaaggcg aattgaactg cccttcgccg ttgccgcgcg agccgaactt catgagatag
     4021 ccgcccagtt tcccatcaaa tttctgaacc cggtggttgt tcgtatccac gacccacacg
     4081 tctcctttgc cgtcgcaggc aatgcccgtc ggattgtgga aatttgcgtt ggctgagccg
     4141 aagttccccc acaggatgat gaagttaccg gcgttatcaa acttttgaat ccgattgttg
     4201 ccgttgtcga ccacaaacaa ggaaccctgc tgatcgatgc acagaccgta gatcggcgcc
     4261 atgaattccc cgccgtgcag gagtgaggcg ccgcggccgg agcgtcccca cctcgacacg
     4321 cagagataac cggatgtatt gaccagaatc gtggcactcg ccggagtgga gacgttgccg
     4381 ccgacgtcct tgaaccatac aaagatcgtc ttctgaccgt cgccaggaga gaggatgaag
     4441 ggaatggtag cgccgaactt caatgatggc gtgacctcca cccacccggg cgtcccggcc
     4501 atcggggtca tgggcgcttc ggatacatag taggccccga cgccggtatc gatgtcgttg
     4561 gctgaaatgg tgaccaccac ttcgggcgaa ttcgtcatga aggcgccgtg attgatcacg
     4621 gcatacgggt tttgcggagc cgtaatgtcg atcagcaccg gcgtggcggt gacaacgcgt
     4681 gacaactcac tttcgacgtc gccgtcgaac atcgccgtca ccgcataata atacggcgta
     4741 tcgttcgtca gtccggtatg cgtgaaggga ttggtcacgc cttcgatctt agttccactt
     4801 tgggtcgtca agagcggcga ggtattgaag tacaggttgt aagagacggc accgggtact
     4861 tcaatccagc tgagaaacgt ctccgtatcg ccagccttta ccgcaacgtt gctcggaggc
     4921 ggcgtcgcat tggttccctg cgacttgggc ctctcttcct tgcccctgtt gatttcctcg
     4981 tcggtcggca cgtacttgag gattcgattg ttgccgctgt ccaccacgta gacgcagccc
     5041 tctttatcga ccgcaatccc gtaaggaaag ttcaattgcc cttcggattt gccgcgattg
     5101 ccccacgcgc acagaaacgt cccatttcca tcgaatttct gaatgcgatg gttgccggta
     5161 tccacgacat agacgttgcc caacgcgtcg caggcgatgc cccatggcgc tttgaactgc
     5221 ccgggtcccg ctccttccct gccccatttg gtcaagaagc taccacgggc gtcgaacttt
     5281 tgaacgcggt tgttgccttc atcggccaca tagatgttcc cgacaaaatc cactgccact
     5341 ccacgcggga aaaagaaggc cccgtcaaaa ctcccgtcgc gaccccattt caagagaggc
     5401 gtcccatccg ctttgaactt ttgtatgcgt gcgttgctgg tatccgagac atacagattg
     5461 ccgtcttgat ccgtcgcgag cccccagggc acatcgaatt tgcccatatc ggcgccacgc
     5521 cacgcaaagc caaacttgcc ccaggcctgc atgacgttgc cgtcgagatc aaacttctgc
     5581 acccggttgt tgccgctgtc ggcgatgtac aggctgtcgt tcgccccgac cgcgaggccg
     5641 cgtggataat aaaagctgcc ttcctgagac gacggctcgc caccccagcg cgcgacgaag
     5701 tgtcccgcct tgtcgaactt ttgaatggag tgattatcgg tatcagcgac gtacagattg
     5761 ccgtccttgt ccagcgcaat gccggtcggc gagctgaatt ctccgtcgtc gacgccttcc
     5821 tggccgatgc acgcggcgag cagataggga gagggaatcg ccatcacttc ctgcgattcc
     5881 agactctcac ctttcgcggt cacgacggtg acgacatagt gatagcaggt gccgttggcc
     5941 agatcatcgt ggatgaaggg agactgtgcc ccctcgagac aggtggcctt ttcctttttc
     6001 actccgatga cggttttgaa atcctctgga ccggcgatgg gacgcgtcag ctcagaaaac
     6061 ttgatgtgaa cgcccttggt cgtctggaag tagagattgt agtacagggc gtcaggaacg
     6121 gggtcccagc taatggtaat ccgcccattc ccgcacttgg ctgctacgtt cgcaggtgcc
     6181 ggagggagtt cctcttcctc tatgatcgaa tcgccggcgc cccattctcc gtgagtgcct
     6241 tccactgtca gcggcgcacg atcgaaccga aacatctcgt ccagcagcca tgctttgatc
     6301 ataggtggtg cctcttgttc ctcgacaggg ttacaaccaa gaaactagca tctttcaatg
     6361 agttacacag gattggggag tctaacaaag cgaaaagctg aaatgcaagg gagagccttc
     6421 cctcccatcg atttcccctc tcatatcgcc gcatagaaca cgtagcctga caggtggccc
     6481 tgcaaggaag tgtccacggt ctcctgagaa ggaaatactg agagtgccgc cactcgtgca
     6541 atgacgtggc cggaccaaca accgagatct ggatcgaaga cttacgtctg ctcgaccggc
     6601 tcagggatca aggcctgcga ttcccaggac tccaagtcct gctcggaatt cggtggctct
     6661 tgcagggtga cttcgtttgc ttccatcggc agcagcgcct gctccgcttc gcccaattcc
     6721 ttaaactcgc gtagcggcgg caactgggtg atgtcctgca aaccgaaatg ttcgaggaaa
     6781 aatttggtcg tcccatacat aatgggacgg cccggcacct ctttacggcc cacgatgcgg
     6841 accagtttcc gttcgagcag cgttctcaag acccctgacg tttcgacacc acgaatttct
     6901 tcaatctctc cccggaccag cggttgtttg tacgcaatga tggccagcga ctcaagcgca
     6961 gaccgggaca atttctgagc cgacttggct ttgtccaacc gcttcaacca gggcgcatag
     7021 tcagacttgg tcacgagtcg atatccgccc gcgagtttca caagctggat tccccggcct
     7081 tcttgagcga gttcctgttc aagcgccttc agcgcttgct caacgtcgga ctttgaaact
     7141 gttccaatca cggtggccaa ccgcgagacc ggcaccggtt ccgtggacac gaacaatagc
     7201 gcttcgacca caccgatcaa ctcacgtcct ttcagcgcct cttcctgtgg ttcaatctga
     7261 gccgcgcccg tttccggcga tgtcgatccc atgaccccat ttccatcgac cgccgagccc
     7321 tcgtccaatg cttccactgt ggctgcatcc tgcagctccg ctaagttcat gcctacctcc
     7381 attccgagtc atccaattcc gccggatccg gcaccaacga gaaggagcgt gacaccaaaa
     7441 tcggcccgaa actttccgcc tgataaacac gcaccgttcg caaacgaatg agttcgagca
     7501 acgctaagaa ggtcacgatg atcagcaacc ggtgagaaga tgcgtcaaac aaagcctcga
     7561 agccgattga ctcttgtcct tccagagctt ccaagatgac attcatccgg tcgcggacgg
     7621 tgagattatc gggaaggatt tccaacagct tcttaccggg attgcgattg agaatcccct
     7681 gcagcgcgtc caccagatcg aacaaaccaa tgttctcgag catcgcctcg tcggtgaccg
     7741 gcgcatcttt ctgaggtgtc gcgggccggc tgtagatctc ccgccacatt ttttcctgcg
     7801 tatccagctg gcgtgccgct tctttgaaag tcttgtactc gagaagcctg cggacgagtt
     7861 cttcgcgggg atccggtccg tcgtcttcgt cttcagcgct ttcttccacg ggaagcagca
     7921 tcttcgattt gatctgaagc aatgtcgcag ccatcaccag aaattcaccg gcgacggcaa
     7981 gattcagctc cttcattgcc tcgatgtagc tcaaatattg ctgagcaatc attgcgatcg
     8041 gaatatcgta gatgttgatc tcgttcttct tgatgaggtg cagcaacagg tcgagagggc
     8101 cctcaaagtt ctcgatacga acttggtagg gcagttcagc ttgttcggcc ccgtcgagct
     8161 ggttatccac agggtggttt ataccagctt tggggagagt gagcaagggc cgatctatta
     8221 gttgtggtta tctggaccga accagatagg cgaccagcac ggcactgaac aggagcagag
     8281 agagggtaat gacgtattgg acctggcgat tctcgatccc cagcgaatca ggattggccg
     8341 cagaccgctt ggctcgggtg aattgcggtc ctttttcgaa aaacgctttg gagaaatctt
     8401 ctcgtgcatt gaattgcgca tcatcgaaat cggccgttgc gcggaatgtc gcgcgccgga
     8461 aaaaagcatc gccatcgaac cgcgtgaagg tgaagaaggc gtccccgtcg aacagcgtct
     8521 cggagaaatc agccagctgc ttgaagtgac ttccggagaa tcctgtcccc agtttgaaat
     8581 aggtccgcga aagattggca tcctgatcga aacgaacttc gaggaactct gccatcccac
     8641 tgaagccgga ttgttgaagc gtcactggcc catgaaactg cgaccggtga aatctcgtgt
     8701 gggagccgaa ggccgttttt tctccggtca cttcacgcag aaaatgacat tgcacgaaat
     8761 acccttcccg taagaacacg gcacccgaca atgcgacggg ttgggtgaaa acagatctcg
     8821 acagatcgac catacgctcg aacgtcgtcc cggcaaacgt caccggaccg gagaagacga
     8881 gtgcgccctc cgccgcacga tgcgtgacgg ttcctcgaac caccgaatcg acgattgcaa
     8941 ccggccccgc aatgactcgg atctctgtgt cgccgtcgcc gatgatgcct ccgacggcag
     9001 gcggattctc actcataggc agggcatcaa gtgcaacgtc tccgcggatc acgactccgg
     9061 caagatcgat ggtctcgcca cgtttcagcg cggccacgag attcgaggca tcgaccgcac
     9121 gagcctctcg ctcgaccggg gtgcagccag cactgagatg ggttttgaag agccgttggg
     9181 aaccagctgc agcactctct acggcgcact ccgccgcggc agaccccgca gagtacagca
     9241 gcacaaccag gagggcgaca gctttaaccg gtcgattcat gacggccctt atacgaaatg
     9301 cctcaggctc ctggcaagac ggggaatgat gttgacttcc tttacgagcc tgaacggtct
     9361 gctagactct ttccatgcat gtgcgcacac tcatcgctgg cacgctctta gttggtgtat
     9421 ttttgcagac tgccattgcc agttacgcca tcgattacct tgaagtaccc gtcctcgcag
     9481 ccgttcgcta taacactctg ggcgcctttg caatcatgtt gatggggtgg gacaaaaagt
     9541 ctgatccgaa tgcggttcaa cttcaggtgt acattgcagg cgttcgttac gggaaaaatc
     9601 acctgggatc catggcccag gcgttcgact acgcgataca gcgcacccct gcagtgatcc
     9661 atacaggaac cgtctttgtc caaggggtta ccgatctgcc aacaacgagt gatggtccaa
     9721 gcgccggtgc ggccat
//
LOCUS       DASTQY010000027         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1592412, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000027 DASTQY010000000
VERSION     DASTQY010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592412"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="VFR82_00935"
     CDS             complement(<1..1411)
                     /locus_tag="VFR82_00935"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU4500190.1"
                     /translation="MKLIHLFLALCLHCICLTSVFAGSPLDASISSSDWLPAALDQIS
                     VERMLADIRVLSGPTLAGRQTGTLEDERSASFVTNRFSELRLHRAPATPLQSLTNPLP
                     QQEWKQTASVPTRRIGEDVLIELVRPQERRSLVTGTQVLPVLDSPSADVRGPIVFVGY
                     GIADPSRGIDDYAGVDVRNKIVLFLRGKPDRYTAVVAQAEKERVAKEKGAIGYLLATG
                     PILTAYEQRRGVTGAPSAFYSTTASTTQLPGAWISTETAEWILEQGATGTTTLRNIQE
                     ELNRTGLSKPVVTDVTAQMRWTSSLETGTLYNVASIIRGHTVNSDETVLVGAHRDHFG
                     RQAGLLFSGADDNASGTAVLLEIARVLSLAPAAPKRSVLFVSFSGEEQGFLGSRLYVT
                     QPIVPLLKTIGMINVDHAGAGNGRLTVGVTGLEKSTAERIGQLTGLSDKLDVFGFFPG
                     GDHVPFKEAGVPTVTVVSGG"
     gene            1525..>3096
                     /locus_tag="VFR82_00940"
     CDS             1525..>3096
                     /locus_tag="VFR82_00940"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU4500191.1"
                     /translation="MSERAFNRLASESSPYLRQHANNPVDWYAWGAEALSRANAEDKP
                     ILLSIGYSACHWCHVMAHESFENAEIAALMNQHFINIKVDREERPDLDDIYQKAAQVF
                     TGRGGGWPLTMFLTPAQEPFYGGTYFPPVPRHSLPAFPDVLRGVLEAYRSHKDEVQKN
                     VERVKGGLLRVGVPTEPSKAVTERLLEQAVSELGQLFDPLHGGFGEGPKFPTVPPLSL
                     MFLQSVRTKDSRLLDMVLLQLRRMAGGGLYDHLGGGFHRYSVDAEWKIPHFEKMLYDN
                     AQLVRLYLDGWRLTKEERFRQVVEETLDYVRRELMHSDGAFFAAQDADSEGREGAYFT
                     WTHDEIVSELGSDLGGEFCRAYGVTAEGNFEGKNVLHRLEGGRLTTEEQEAAESVLKP
                     AREKLLAARERRVKPQRDENILTGWNAMMISAMFDAALTLNQPTYRAAAEKALTFLLD
                     YAFVHGRVYHTVVEGKGRLNGYLDDAAWLAVALLDAFEATSHRWYLDQARLVTDSIES
                     NFWDEAGGGGCFFTSH"
BASE COUNT          655 a          846 c          931 g          664 t
ORIGIN      
        1 cgcccccgct gaccacggtt acggtcggca caccagcttc cttaaacggc acatgatcgc
       61 cgcccgggaa aaagccgaac acatccaact tgtccgagag accggtcagc tgaccgatcc
      121 gttctgccgt gcttttctca agacccgtga ctcccaccgt taaccgacca ttgccggccc
      181 cggcatgatc gacgttgatc atgccgatcg ttttcagcaa cgggacgatc ggttgtgtca
      241 catagagtcg cgagcctagg aaaccctgtt cttccccgct gaacgacaca aagagaaccg
      301 accgcttggg tgctgccggg gcaagagaaa ggactcgcgc gatttcgagc agcacggcgg
      361 tgccagatgc gttatcgtcc gccccggaga ataacaaacc cgcttgccga ccgaagtggt
      421 ctcgatgcgc tcctaccagc acggtctcat cgctattcac cgtatggcct cgaatgatgg
      481 acgcgacgtt atagagggtc ccggtctcaa gactgctcgt ccatctcatc tgggccgtga
      541 catcggtcac cacaggcttt gagaggccgg tgcggttgag ttcttcctgg atgttacgga
      601 gcgtcgttgt tcctgttgct ccctgttcga gaatccattc ggctgtctcg gtgctgatcc
      661 acgcgcccgg caactgtgtg gtgcttgcgg tcgtggagta gaacgcactg ggcgcacctg
      721 tcacgccccg gcgttgttca taggcggtta agatgggccc ggtggccagg agatagccga
      781 tcgccccctt ctcctttgcc acacgctcct tctccgcctg cgccacgact gcagtatacc
      841 gatcgggttt gccccgaaga aataaaacga ttttgttgcg tacgtcgacg ccggcataat
      901 cgtcgatgcc gcgagatgga tcggcgattc cgtaaccgac gaacacgatt gggccacgga
      961 catcggccga gggagagtcg aggacgggca aaacctgcgt gcctgtcaca agcgagcgtc
     1021 gttcctgcgg tcgcacgagc tcaatcagca cgtcctcgcc gattcttcgc gtgggcaccg
     1081 aggcggtttg tttccattct tgttgtggga gcgggttggt caggctttgc agtggggtcg
     1141 ctggtgctcg atgcaggcgg agttcggaga accgattagt cacgaacgag gctgaacgtt
     1201 cgtcttccag ggtgcctgtt tgccggcccg caagggtcgg cccactcaat actctgatat
     1261 ccgccagcat gcgctcaacg gagatctgat ctaatgcggc aggaagccaa tcggatgacg
     1321 agatcgatgc gtcgagcgga gagccggcaa aaacagaggt aaggcagatg cagtgtaaac
     1381 agagagccag gaataggtga atgagcttca tggcgtccac atgttgaccg gcactatagc
     1441 acggactgtg agaatcattg caggggagga gtcggcaaga tactatggcc agcgccgagc
     1501 ggaaggattc attttcgatc catcatgagt gaacgagctt tcaatcgcct cgcttccgag
     1561 tcaagtccct atcttcgaca gcacgcgaac aatccggtgg actggtatgc ctggggtgca
     1621 gaagcgctct cccgtgccaa tgcggaagac aaacctatct tgctctcgat cggttattcc
     1681 gcttgtcatt ggtgccacgt aatggcacat gagtcgttcg agaacgctga gatcgctgca
     1741 ctgatgaatc agcattttat caacatcaaa gtggatcgag aggagcgtcc ggatctcgac
     1801 gacatctacc aaaaggccgc gcaggtattt accggtcggg gcggcggttg gccgctcacc
     1861 atgtttctga cgcccgcgca agaacccttc tatggaggca cctactttcc accggtgccc
     1921 cgccactctc ttccggcctt tcctgacgtc ttgcgtggag tgctcgaagc ctaccgtagt
     1981 cataaagatg aagtgcaaaa aaacgttgag cgcgtgaaag gcggtttgct cagagtgggg
     2041 gttcccacgg agccgtccaa ggcggtgacc gagcgacttc tggaacaggc ggtcagcgaa
     2101 cttggtcagc tgttcgatcc tctccatggc ggcttcggtg aagggccgaa atttccaacc
     2161 gttccaccgt tgagtctgat gtttctgcag tctgtcagga cgaaagactc aaggctcctc
     2221 gatatggttc ttcttcagct gcggagaatg gcgggtgggg gcctgtacga tcatcttggc
     2281 ggagggtttc accggtactc cgtcgatgca gaatggaaaa tcccacattt cgaaaagatg
     2341 ctgtacgaca acgcccagct tgtgcggcta tatctcgacg gatggcggct gacgaaggaa
     2401 gaacggttcc gccaggtcgt ggaagagacg ttggactatg tacgacgtga actgatgcat
     2461 tcggacggcg ccttctttgc cgcacaggat gccgacagtg aaggccggga aggtgcatac
     2521 tttacgtgga cccatgatga aattgtatct gagcttggat cggacttggg cggcgagttt
     2581 tgtcgcgcct acggcgtcac ggcggagggg aatttcgaag ggaagaacgt gcttcaccgg
     2641 ctcgagggag gacgtctgac caccgaagaa caggaggcag ctgaatcggt attgaagccg
     2701 gctagggaga aattattggc ggcgcgagag cgacgggtca agccacagcg tgacgagaat
     2761 attttgaccg gttggaatgc catgatgatc tctgccatgt tcgacgctgc attgacgttg
     2821 aaccagccga catatcgcgc tgccgccgag aaggcgctga cgtttctcct agactatgcg
     2881 ttcgtgcacg ggcgcgtata ccatacggtt gtcgaaggga agggacgact caatgggtat
     2941 ctcgatgacg cggcctggct agcggtcgca ctgctcgatg cgttcgaagc cacctctcat
     3001 cgctggtatt tggaccaggc ccggctcgtg actgactcaa tagaatccaa tttctgggac
     3061 gaggcaggcg gcggaggctg tttctttacc agtcac
//
LOCUS       DASTQY010000028         5828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1391776, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000028 DASTQY010000000
VERSION     DASTQY010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391776"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VFR82_00945"
     CDS             complement(<1..974)
                     /locus_tag="VFR82_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU4500192.1"
                     /translation="MSQQNGLPALVIRSFLPEAREAVVLSQASDELAFPMTRIHESGL
                     FEAVLPPAPAPAHYRLRVVDHAGRLSERYDPYAFAPLLSDFDLHLFAEGTFYKAYETL
                     GSHVRRIDGITGIHFVVWAPNAGRVSVVGDFNQWDGRRHPMTNRGATGLWELFIPGLE
                     DGTLYKYEIRPRGRDGVLLKADPYAVASELRPKTASVARDISAYRWNDQAWMENRRRQ
                     NPLKEPVTIYEVHLGSWMRIPEEHNRWLTYRELAAKLIPYVKDMGYTHVELMPVTEHP
                     FDASWGYQATGYFAVTSRYGTPEDFMAFVDAAHQAGIGVLMDWAPAHFP"
     gene            1219..2559
                     /locus_tag="VFR82_00950"
     CDS             1219..2559
                     /locus_tag="VFR82_00950"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEU4500193.1"
                     /translation="MHPSELSIALFIFGLAYAVIITERIHKTIVAVSGAALMIASGVV
                     TQDEAFYSHEFGVDYNVVFLLIGMMVIVNIVRETGLFEVLAIWSAQRAGAKPLRLLMM
                     LAIVTAALSAMLDNVTTVLLMAPVTLAITKRLNLNPVPYLIIEAVASNIGGTATLVGD
                     PPNIMIASKAELGYVDFLVVLGPIVTVIMAVFLGCLKLFFGAMQVAPHQANAVMQLDA
                     SDAVQDPVLLRRCVWLLVLINVAFCIHGALHLEPATIALVGASAFMLIGHATKKADAD
                     ELSYLTDVEWKTIFFFIGLFILVGGLVKVGVIRFLAGQLVSFTQGHPAATTLSVLWGS
                     AILSAIVDNIPYVAAMNPLIVDLARSAHPEVADYTTLVHQPDILPLWWALALGACLGG
                     NGTIIGASANVVIVDLARKAGYPISYWDFFKLGFPVMVGSVLLSTLYVWLRFLR"
     gene            complement(2605..2832)
                     /locus_tag="VFR82_00955"
     CDS             complement(2605..2832)
                     /locus_tag="VFR82_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500194.1"
                     /translation="MSDLPLTYRCPACQRVKKQHWDGSAGQDWCTMADYVQRCFIQEH
                     DVLLSEFYCADCTLSYDRLVHYSRTNPSCFT"
     gene            complement(2940..3227)
                     /locus_tag="VFR82_00960"
     CDS             complement(2940..3227)
                     /locus_tag="VFR82_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500195.1"
                     /translation="MLLERRSRFGRIVCYGIGLVCLCLVMYVLGSTMTLWTMEFPLDQ
                     TDGPLLEGLSLPTTLSDLVPALSASSAGESSATALRQLSEHGLLRPPIATA"
     gene            complement(3295..5571)
                     /locus_tag="VFR82_00965"
     CDS             complement(3295..5571)
                     /locus_tag="VFR82_00965"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEU4500196.1"
                     /translation="MCLAVVVMFAVFGLRPHAEGQLKVSDQPSEKASDAASKPPTDDA
                     SSLSELRAKRDKAQAELDAVNRPKALATGAPPGTPEPELLERRTLLHHIVRSADEQID
                     ATLRLEQARQHRDEVGRSLPDSKDLGEAPPTIFDADRLWDTAFALRLAVEGLQSQLAL
                     IELRSTRAKDSLQAAEERFRQASEHLESTTSPSGADRNRWLRDLEALRQRAAAALLQA
                     TELSKIRIGDELSDARARLQTAESRLSTVDPLVDFTEPDLEKIRTRLSQERLGLTHEL
                     EETTTARHRQFEALRDLERQLASRVAKGNGKAGGKPPESLRRIQAGTEVGRIQADNLA
                     LKSDLWQLLLDVVDSERQLWESRFAIMHNPEPGKAREAYDRFTPLFTNLQASRDSVRQ
                     QLSIVSGQIGELESRVKNSRPGQDRNHVRALLEAHREREAIYNYGLQRLDQTSRFLER
                     WKSAFKGQQKELPMTARVEEWWQRAWDRAKTTWHWEVFDAEDTIEVDGKKITGHRSVT
                     VGKIVTALIILTIGYWLCLYLATQIGRLAVNRLAMAPEVANLLRQWSQALLLTILIVT
                     SLVWVKIPLTIFAFLGGAFAIGVGFGAQNLLKNVISGILLLIERPLRVGDLIEVENVR
                     GRVTTIGLRSSTVRDAKGVVTLIPNSSFLERHLTNWTYSSRIGRFSLRVGAPYDSSTQ
                     LVMDLLAGLAASHRQVLKTPKPQVLLDEFGNQGRIFTLNYWLEIRLDVDPNEVASELR
                     FGIEQKFVEAGLKILPAA"
BASE COUNT         1173 a         1616 c         1715 g         1324 t
ORIGIN      
        1 ggaaaatgtg cgggagccca atccatcaag acaccgattc ctgcttgatg cgccgcatcg
       61 acgaaagcca tgaaatcttc cggagtgccg tagcggctcg tcaccgcaaa atacccggtc
      121 gcttgatagc cccaggaagc atcgaatgga tgttcagtca caggcatgag ctccacatgc
      181 gtgtatccca tatccttcac gtagggtatg agttttgcgg ccagctcacg atacgtcaac
      241 cagcggttgt gctcttcggg aatgcgcatc cacgagccga ggtggacttc atagatggtg
      301 acaggttcct tgagcggatt ttggcggcgc cggttctcca tccaggcctg atcgttccac
      361 cgataggctg agatgtcccg tgcgactgat gcggtttttg gtcgcagctc cgatgcgacg
      421 gcataggggt ctgctttcaa caaaacaccg tcgcggccac gagggcggat ctcatacttg
      481 tataaggtgc cgtcctcgag cccggggatg aacagttccc agaggccggt tgctccgcga
      541 ttggtcatgg gatgccgccg accatcccat tgattgaaat cccccaccac actcactcgg
      601 ccggcattcg gggcccagac aacaaagtgg attcctgtaa tgccgtctat cctgcgcacg
      661 tgcgatccga gggtctcata ggctttataa aaggtgcctt cagcaaacag atgtaaatcg
      721 aagtctgaaa ggagcggggc gaaggcatac ggatcgtagc gctcggacag ccggcccgca
      781 tgatcgacca cgcggaggcg ataatgcgcc ggcgccggcg caggcggcag gaccgcttcg
      841 aagaggccgg actcgtgtat acgggtcatg ggaaatgcca gttcgtcgga ggcttgggac
      901 aacaccaccg cttcacgggc ttcgggtaaa aagcttcgaa tgaccaaagc aggtaggcca
      961 ttctgctggc tcatgtgagg tccaagaata gccagggggt cccaatgtga accagtgact
     1021 aattgttcga tgtgctgtcg tgacaacgcg gacatgacgt tcacactcct cgtacaaacc
     1081 tgcgatcgag ccggtccaga gccactatta tgaaagaaga aagagcagat tgccacaagc
     1141 cgctcggctc acgtccattg caagagccgg ccgtcatctt gataatgcga cgaccctaac
     1201 cgcacaggag ccagcgctat gcatccgtcg gaactctcca tcgccctctt catctttgga
     1261 ctggcctatg cggttatcat taccgagcgg attcataaaa ccatcgtggc ggtgtccggc
     1321 gcggctctga tgatcgcgtc cggcgtggtg acacaagacg aagcgtttta ttctcatgaa
     1381 ttcggcgtgg actataacgt cgtctttcta ctcattggga tgatggtcat tgtcaatatt
     1441 gtccgtgaaa cgggactctt cgaagtgctc gcgatttggt cggcccagcg tgccggagcg
     1501 aagccgttac gattgctcat gatgttggcc attgtcaccg ctgccctgtc cgccatgctg
     1561 gataatgtga cgacggtgtt gttgatggcc ccggtcacgt tggcgatcac caagcgtctg
     1621 aacctgaatc cggtgccgta tttgatcatc gaggcggtcg cctcgaacat cggcggaacc
     1681 gcgacgttgg tgggggatcc tccaaacatc atgatcgcca gtaaggcgga gcttggctat
     1741 gttgactttc tcgtcgtcct tggaccaatt gtgacggtca tcatggcggt attcctcggt
     1801 tgtctcaagc tgttcttcgg tgcgatgcag gtggctcctc atcaggccaa cgcggtgatg
     1861 caattggatg cgagcgatgc cgtccaggat cccgtgctgt tgcgacggtg tgtctggctt
     1921 cttgtgctga tcaacgtggc gttttgcatc catggtgcgc ttcacctcga gccggcgacg
     1981 atcgcgttgg tgggggcgag cgcattcatg ctcatcgggc atgccacaaa aaaggcggat
     2041 gcggatgagt tgtcgtatct cacggatgtg gaatggaaga ctattttctt ttttatcggt
     2101 ctctttattc tcgtcggcgg tcttgtgaaa gtcggcgtga tacggttcct tgccggtcag
     2161 ttggtatcct ttacgcaggg ccatcccgca gccaccaccc tttcagtgct ctggggctcc
     2221 gccattctct ctgcgattgt cgacaatatc ccttatgtcg ccgcgatgaa tccattgatc
     2281 gtcgatctgg cacgctcggc ccaccccgag gttgccgact acaccacgct ggtccaccaa
     2341 ccggacattc tgcctctctg gtgggcgctg gccctcggag cgtgtctagg aggtaatgga
     2401 acaatcatcg gcgccagcgc caacgtcgtg atcgtggatc tggccagaaa agccggttat
     2461 cccatctctt actgggattt ttttaagctc ggatttccgg tgatggtggg gtcggtgctg
     2521 ctgagcacac tgtatgtatg gctgcgcttt ttaaggtgac accagccccg ctgaggcgcg
     2581 aggctggtct ctccatcccg catgttacgt aaagcacgag gggttggttc ggctatagtg
     2641 gacgagccgg tcataggaca gggtgcaatc tgcacaataa aactcggaca gtaagacgtc
     2701 gtgttcctga atgaagcagc gctgaacata atccgccatc gtgcaccaat cctgacctgc
     2761 tgatccatcc caatgctgtt tcttgactcg ctgacaagct ggacaacggt atgtcagtgg
     2821 tagatcgctc atgttcgact cctgtggctt gccgtgctcg attccctcgg gaaagtgtgc
     2881 gaacgtcatg agcgttgcgc agctgggtgc cgcaatgcca tgaacagaga atgagaggat
     2941 caggcagtgg caatgggagg acgcagcaag ccgtgctcgc tcagctgcct gagggcagta
     3001 gccgagctct cgccggctga tgaagccgag agcgcaggga cgagatcaga aagagtggta
     3061 ggcagtgaaa gaccttcgag aagcggaccg tctgtctgat cgagtggaaa ttccattgtc
     3121 caaagcgtca tggtgctgcc gagcacgtac atgaccaggc agagacacac cagcccgatg
     3181 ccatagcaca cgattctgcc gaacctcgat cttcgttcaa gcagcatggg cctgctccaa
     3241 gagggggaca gtctaggatc gcgccattca tatgtcaatg ccatggcggg aagatcatgc
     3301 ggccggcaaa atcttcaagc cggcctcgac aaacttctgt tcgatgccga agcgcagctc
     3361 actggccact tcgttaggat caacgtccag cctaatctcg agccaataat tcaacgtgaa
     3421 gatgcggcct tgattgccga attcatcgag caacacttgc ggttttggcg ttttcaagac
     3481 ctgacgatga ctcgccgcga gtccggcgag aagatccatc accagttggg tggatgagtc
     3541 atagggagcg ccgacacgaa gggaaaagcg accgatgcgg ctcgagtagg tccagttggt
     3601 gagatggcgc tcgagaaaac tgctgttcgg aatcaaggta acgacgcctt tcgcatctcg
     3661 gacggtggaa gaccgcagac cgatggtcgt cacgcgcccg cgcacgttct ccacttcaat
     3721 gaggtcgccg acccgcaagg gtcgttcaat cagcaggagg atgccgctga tgacgttttt
     3781 caataggttt tgagcgccga atcccactcc gatggcaaaa gcgccgccca aaaaggcgaa
     3841 aatggtcaat ggaatcttga cccagacgag gctggtcacg atcaggatgg tgaggagcag
     3901 ggcctggctc cactggcgca gaagattcgc cacttcgggg gccattgcga ggcggttgac
     3961 tgcgagccgg ccgatctgcg tggccaggta cagacagagc cagtatccga tcgtgaggat
     4021 gatcagcgcg gtcacgatct tgccgaccgt gacgctgcga tggccggtga ttttcttgcc
     4081 gtctacctcg atcgtgtctt cggcgtcgaa cacttcccag tgccaggtcg tcttggcgcg
     4141 gtcccaggcg cgctgccacc actcctcaac acgggccgtc atgggaagtt ctttctgctg
     4201 tcctttgaat gccgacttcc aacgctcgag gaatcgagag gtctggtcga gacgttggag
     4261 gccgtaatta tagatcgctt cgcgttcccg gtgtgcctcc agcaatgccc gtacgtggtt
     4321 gcggtcctgg ccgggcctgc tgtttttgac acgcgactcc aactcgccaa tttgccccga
     4381 gacgatgctc agttgctgcc gcacagaatc ccgtgacgcc tgcaagttcg tgaagagtgg
     4441 cgtaaagcga tcataggctt ctcgggcttt tcccggttcg gggttgtgca tgatggcgaa
     4501 gcggctttcc cacaactgcc gctcgctgtc taccacgtcg agcagcaact gccacagatc
     4561 gctcttcagt gccagattgt cggcctggat gcggcctact tcagtgccgg cttggatccg
     4621 tcgaaggctc tccggcggtt tccctccggc ttttccgtta cccttggcca cccgggaagc
     4681 cagctgtcgc tcgaggtccc gcaatgcctc gaactggcgg tgacgtgcgg ttgtcgtttc
     4741 ttcaagttcg tgcgtcaacc ctagccgttc ttgtgacagg cgcgtcctga ttttctcgag
     4801 atcgggttct gtaaaatcaa ccagcgggtc caccgtcgac aagcgactct cagccgtttg
     4861 aaggcgtgcg cgcgcgtcgg atagctcgtc tccgatgcgg attttggaaa gttccgtcgc
     4921 ttgcaacaat gccgctgcgg cgcgttggcg aagcgcttca aggtcgcgta gccagcgatt
     4981 ccggtcggct cctgaaggac ttgtcgtgct ttccaaatgt tcagatgctt ggcgaaagcg
     5041 ttcctcggcg gcctggagag aatcctttgc ccgagtggag cgcagttcga tgagagccag
     5101 ttgggactgc aggccttcga cggccaggcg cagggcaaag gccgtatccc acaatcgatc
     5161 ggcatcaaaa attgtgggcg gagcctcgcc cagatccttc gagtcgggca gagaccgtcc
     5221 gacctcatcc cggtgttggc gagcctgctc gagacgcagc gtcgcatcaa tctgctcatc
     5281 ggcgcttcga acgatatggt gtagcagggt ccgccgttct aaaagttccg gttccggcgt
     5341 acccggagga gcgccggtcg ctaaagcttt gggccggttg accgcatcca gttctgcttg
     5401 tgctttgtct cgcttggccc gcagttcact cagcgacgac gcgtcgtcag ttggtggttt
     5461 tgaggccgca tcggaagctt tctcgctcgg ttgatcgctc accttgagct gtccttcggc
     5521 gtgcgggcgc aagccgaaga cggcgaacat cacgaccacc gcaagacaca tgcgaatgtg
     5581 catagaagga atggaatggg atggatctat ggttggtacc acggctggaa gggttgaagg
     5641 actggtgaaa ccccttggga ttgtgtccgc cgtttctgtt ctcacgtagt aatcccgttt
     5701 cgatcttcgg atctttcctc atatgaattg tgtccgtcat tctatctctc aaagaatctt
     5761 cctcgggcat ttgctagtcg atcaccgtct gagcctccat catagtgaat gaagtggtgc
     5821 gcgcgttg
//
LOCUS       DASTQY010000029         3481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1393213, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000029 DASTQY010000000
VERSION     DASTQY010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393213"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFR82_00970"
     CDS             <1..492
                     /locus_tag="VFR82_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500197.1"
                     /translation="RQEMLECPSMCKGLLRLLPLLMWAIGLGFAVVSPASGADPTAIR
                     DILDEPSVYHLRQVVLQGRVRNVQPLDPYKLPAGTTCYGAYLFYLEDETASINVAVFG
                     LCGVPTVKDPDVEEGTQVQLQATIQAPSHGGYYLSFQGLTVAGEREGVIQAVADRITP
                     LME"
     gene            493..1236
                     /locus_tag="VFR82_00975"
     CDS             493..1236
                     /locus_tag="VFR82_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500198.1"
                     /translation="MISAPEFSMSLGARPPRRTRWVLFLCAVLVGSVGCASSHGLSRD
                     SLQDVILREEAGFLDPPSAAAAPAPRHAPTLGFYFKPTAFVHREFEWTSRDRDAVLTW
                     AQGLSLGEGGRSTGFLTPSSLKGQSLRELRATAARYGVDWVLVFDGAAAVDRYNNYKA
                     PLLYWTVVGAYLAGGTHSDALCLMKATVWDVKTGARLFDEHADAITRTVGPAALVDDG
                     REIERAKTRAMETLLQRLTDRFALLVGRR"
     gene            1236..2417
                     /locus_tag="VFR82_00980"
     CDS             1236..2417
                     /locus_tag="VFR82_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4500199.1"
                     /translation="MPPIDVCNAVAVRTVFLLSLITPVCSMAQTPVSEPPPVEKSSDW
                     HLSAYADLAYLLDFNFPENHLWRSRSTSQRVNELAPNMGFLNLTKEMTTDSRWGMEVG
                     IQGGYDAKTFGFLQGEPRVGGSDTLRHIQRANVSYLAPVGNGLSIQAGLFNSLIGYES
                     FFAKDNANYTRSWIADNTPYMMFGVNARYPVNDRLTIAGFIINGYYHLSRPNDQPSYG
                     TQIVYRPAAEVTVTETLYAGPDQNKTAFEFWRFYLNSIVERKTDDFILAFSYDIGTES
                     IANQPGAPRTFVTGGNVVARWRIDSHWALALRPEFYWDRNGRWTGHEQFLKAVTTTVE
                     YAVPFGWTRTRARLEYRWDESVGAGGGFYKGGDVAPGTPGLTASQHLLIMALLWSFYS
                     P"
     gene            complement(2440..2919)
                     /locus_tag="VFR82_00985"
     CDS             complement(2440..2919)
                     /locus_tag="VFR82_00985"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HEU4500200.1"
                     /translation="MQKPARPLARIMLLRVVFVLLSSQSDASHAAPSDGRFSLVMDGA
                     AVKDNQTGLIWEQEPDREHDVWSRSNERCAAKEVGGQKGWRGPSVEELKTLIDPAQRD
                     PALPAGHPFSNIKSEIYWTSTPDPKDDIVAWQISFFSGEPVTDQKSGTRRMWCVLAK"
     gene            3062..>3481
                     /locus_tag="VFR82_00990"
     CDS             3062..>3481
                     /locus_tag="VFR82_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR1/FUN34/YaaH family transporter"
                     /protein_id="HEU4500201.1"
                     /translation="MKDEERRRRIDVLAIGLFGLAVGALTLGIAQVGAISESNKVGTL
                     VIALMFGGVVQILAGITDIQYHEQLGGTALTMYGFFWTTVCIAKLVSESTSFHLDMTL
                     YAPINFVYAAFSAVMVYLTAYRNVTLCMLHIVITVTFS"
BASE COUNT          699 a         1020 c         1005 g          757 t
ORIGIN      
        1 cgtcaagaaa tgctagaatg cccatcgatg tgcaaaggcc tgctccgcct tctcccgctc
       61 ttgatgtggg caatcggtct cggtttcgcc gtcgtctcgc ccgcctcagg agctgacccc
      121 accgcgatcc gcgacattct cgatgaaccg agcgtctacc acctcagaca agtggtgctg
      181 cagggaagag tccgcaacgt tcaacccctt gatccttaca agctgccggc gggcacgacc
      241 tgttacggcg cctatctttt ttatttggaa gatgagacgg cgtcgatcaa cgtcgccgtc
      301 ttcgggctct gtggcgtccc gaccgtcaag gatccagacg tcgaagaagg aacacaggtc
      361 caacttcagg cgaccattca agctcccagc cacggcggct actacttgag ttttcaaggt
      421 ctcacggtcg ctggagaacg ggagggcgtg atccaagccg tagccgaccg catcacgccg
      481 ctcatggagt aaatgatctc cgcaccggag ttcagtatgt cgttaggtgc gagacctcct
      541 cggagaaccc gatgggtcct tttcctttgc gccgtccttg tgggatccgt cggctgcgcc
      601 agcagtcatg gtctctcccg tgactctctt caagacgtga tcttgcggga agaagccggt
      661 tttctcgacc caccgtcggc agcggccgct cctgctccgc gccatgcgcc caccctcggt
      721 ttctatttca aaccgacggc attcgtgcat cgggaatttg aatggacgag tcgcgaccgc
      781 gatgcggtac tgacctgggc tcaaggactc tcgctgggcg agggagggcg gagcaccggc
      841 tttctgacac catcctcatt gaaaggtcag agcttgcggg agctccgggc cactgctgcg
      901 cgctatgggg tcgactgggt gttggtgttt gacggggccg ccgccgtcga ccgctataac
      961 aattacaagg cacccttgct gtattggacg gtcgtgggcg cgtatttggc cggcggtact
     1021 cacagcgatg cgctgtgtct catgaaggcg acggtgtggg acgtcaagac cggagcccgt
     1081 ctcttcgacg aacacgccga cgcaatcacc cgcaccgtcg gcccggcggc attagtcgac
     1141 gacggccggg agattgaacg agcgaagacc cgagcaatgg aaacgttgct gcagcggctc
     1201 actgaccggt tcgccctgtt ggtcggaagg cgttgatgcc gccgatagac gtctgcaatg
     1261 ccgttgcggt ccggacggtg ttcctgctct cgctgattac accggtttgc agcatggctc
     1321 aaacgccggt cagcgaaccc ccgcccgtgg agaagtcatc cgactggcat ctgagcgcgt
     1381 atgcagacct cgcctacctg ctggacttca attttccgga gaaccatctg tggcgcagcc
     1441 gcagcacgag tcagcgagtc aacgaactcg ctcccaacat gggctttctc aatcttacga
     1501 aagagatgac gaccgattcg cgctggggga tggaagtcgg catccaaggc ggttacgacg
     1561 caaaaacctt tggcttcctg cagggcgagc cgagggtcgg gggctcggat accctccggc
     1621 acatacagcg ggcgaacgtg tcctatctcg cccccgtcgg aaatggcttg agcatacaag
     1681 ccgggctgtt caacagtttg attggatatg aatcgttctt tgccaaagac aacgccaact
     1741 acacccgttc gtggatcgcg gataacacgc cctatatgat gttcggagtc aatgcgcgct
     1801 atcctgtgaa cgaccggctg accatcgcgg gcttcatcat caacggctac tatcacctgt
     1861 cccgtccgaa tgatcagccg agctatggca cgcaaatcgt gtacagaccc gccgcagagg
     1921 tcaccgtgac cgaaacgctc tacgccggcc cagaccagaa taagacagcc ttcgagtttt
     1981 ggcggttcta tttaaacagt atcgtggaac ggaaaactga cgatttcatc ctggcgttta
     2041 gctatgacat cggaaccgaa tctattgcga atcaaccagg tgcacctcgg acattcgtca
     2101 cgggaggcaa cgtggtcgct cgttggcgca tcgatagtca ttgggccctc gccctgcgac
     2161 cggaattcta ttgggatcgg aacggcagat ggacgggaca cgagcagttc ctgaaggcgg
     2221 tgacgacgac agtggaatat gcggtgccgt tcggctggac gaggactcga gcgaggctag
     2281 aataccggtg ggacgaatcc gtcggggccg gaggcgggtt ttacaagggc ggcgatgtgg
     2341 cgccaggaac accgggtctc acggcctccc agcatctttt gatcatggcc ttgctgtggt
     2401 ccttctactc accgtgacag acaacggcgt cacaaccgac tactttgcca ggacacacca
     2461 catccggcgt gtgccagact tttgatccgt taccggttcg ccgctgaaga agctgatctg
     2521 ccaggcaacg atgtcatcct ttggatcagg cgtgcttgtc cagtaaattt ctgatttgat
     2581 attcgaaaag ggatgaccag ccggtagggc cggatcacgc tgggccggat cgatcagggt
     2641 cttcagctct tctacagaag gaccgcgcca ccctttctgt ccgccaactt ccttggccgc
     2701 acagcgttcg ttggagcgac tccacacgtc atgttcccgg tccggttcct gctcccaaat
     2761 caatccggtt tgattgtctt tcaccgcggc gccgtccatc acgagagaga accggccatc
     2821 gctcggtgcg gcatgactcg catcggactg ggacgagagc agaacgaaca caacgcgcaa
     2881 gagcatgatc ctcgcaagcg gacgcgctgg cttctgcatg gttggctcgc cttcttccgg
     2941 gtacgaggaa gagtgtgacg aatgcggctt gtcttttcaa tagtcagtca ggcatgctct
     3001 gcgcgcttgt gccggctcca gcttcgcacc tgtttcctat cagaccatga aggaggcgtt
     3061 catgaaggac gaggaacggc ggcggaggat tgatgtcctc gccatcggat tgtttggact
     3121 ggcggtcggg gcattgacac tcggcatcgc acaggttggg gcaatttccg agtccaataa
     3181 ggtcgggacc ttggtcattg cgctgatgtt tggcggcgtc gtccagatat tggccggcat
     3241 tacagatatt caatatcacg aacaactcgg cggcactgcc ctcaccatgt atggattttt
     3301 ctggacgacc gtttgtatcg cgaagctggt cagcgaaagc acgtcattcc acctcgatat
     3361 gacgctctat gctcccatca atttcgtcta tgcggcgttt tcggccgtca tggtttacct
     3421 cacggcctac cgcaatgtga cgctctgcat gctccacatt gtcattacgg tgaccttttc
     3481 c
//
LOCUS       DASTQY010000030         5860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1795176, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000030 DASTQY010000000
VERSION     DASTQY010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795176"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VFR82_00995"
     CDS             <1..906
                     /locus_tag="VFR82_00995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4500202.1"
                     /translation="ATIMASLSFGALCVAGWLLSLWQHHALAWIGFAVIFTLAGVARM
                     CSAVALSKVEDVHHTSHTDPTPGFRTFLAGTSLSFRRFLLFSGLMHAAVLIAAPFFVL
                     YMLRDLHLAYWSYGTWLAAGILGQLVTLGAWGRFADRFGNKALLSVTGFMVPFLPMCY
                     LAGTNLPFLFCVNFLGGVTWGGLALGLQNYVFDAVRPADRAKAVATYSTINALGWCAG
                     AFVGSWLVNLLPSQIAIGQFSVPLASNLPLVFFVSGVLRLAVSGSLLGAFHEARAVEH
                     APFTQLVWELPLLKPLAQFLASPGK"
     gene            complement(923..1291)
                     /locus_tag="VFR82_01000"
     CDS             complement(923..1291)
                     /locus_tag="VFR82_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500203.1"
                     /translation="MQGGSLVEDTAPIGAQSIDPGANLVVLPGRGEYDASGQGCVGRC
                     LFYEGGLYMALRVQCWKSLPNMVCSLAIAGVFLTVAGCSSHEPKPMAQPTPDQVRGHA
                     DHTFDKLKQEERERSSQGPR"
     gene            1301..1747
                     /locus_tag="VFR82_01005"
     CDS             1301..1747
                     /locus_tag="VFR82_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEU4500204.1"
                     /translation="MSLRDRLTEDLKLAMKARDQLRMDVIRMIKAAVLNKEVELKKDL
                     DDAEMSRIMTTMIKQRKESVEQFEKGQRAELAAKERQEISIIETYLPQALSAEELAQT
                     VDAVIRETGATSAKDMGLVMKAVMARLAGQPVDGKSVSDLVRSKLK"
     gene            1829..2467
                     /locus_tag="VFR82_01010"
     CDS             1829..2467
                     /locus_tag="VFR82_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4500205.1"
                     /translation="MSILIVDDSPDQQALLRAILNKAGHKDLVSADGATSAGKVLNLN
                     GDSPTEHIDLILMDVLMPGQDGVETCRQIKRCAHLQDIPVIMVTAKSDLSNLQEAFAA
                     GAMDFISKPVSSIELLARVSSALLLKQEMDRRKNRELELRRSNEELQKALKEVKVLRG
                     LIPICASCKKVRNDGGFWQQLEEYLGEHSEAEFSHGLCQPCIKKLYPGVYQD"
     gene            2525..3529
                     /locus_tag="VFR82_01015"
     CDS             2525..3529
                     /locus_tag="VFR82_01015"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU4500206.1"
                     /translation="MSILIVDDSPDDRTLLQSILTAAGYEHILVAESAVTAFHLLGLD
                     GGKQGAARVDLILMDIIMPEMNGIEACRQIKAVERFRETPIMMVTVKTDPVDLQLAFA
                     AGALDYIGKPINKIELLTRVRSVLRLVHEIERRRAREQELLEVMRQLQEANQMLLRLS
                     SLDGLTGITNRRQFDDFLDQEWRRAVRESTPLSLIMLDIDRFKIYNDTNGHTAGDECL
                     RQVANAISGAVNRPGDLVARYGGDEFVVVLPGTATDGAAQVAETLRKRIETLGITHSE
                     GDHITISVGYASIIPNRNASPTDLIKAADQALYQAKQEGRNRAKQAGILTLVPHSDDE
                     "
     gene            3558..5297
                     /locus_tag="VFR82_01020"
     CDS             3558..5297
                     /locus_tag="VFR82_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4500207.1"
                     /translation="MPRPHAPSRKPPRTSRRPTLAQTQTALRREQAARRREALQHRQT
                     EQALVVAKEAAEVANKAKSDFLASISHEIRTPMNAIIGMADLLWETDLTPEQRKYLRI
                     FRRAGGSLLQLINDILDLSKVEAGHIELESIEFDLGDLVEKAIEILAMRANEKGLELA
                     CHLAPDVPCSLIGDPNRLHQILVNLISNAIKFTETGSVIVEVTNDADEAGPGAIRFSV
                     TDTGIGIPQDKLSTIFERFTQAHASISRQFGGTGLGLTISKQLVGLMHGTIWAESTPG
                     QGSIFHCRVKFGINPSATIRPAACPLDLHDLRTLVVDDHAMNRKILMETLKSWNADVT
                     TVSDGQQALAELRQAALSSRPYGLLLVDCHMPGMDGFQVVEALRHSAMAEGVAIIMLA
                     SDHWADDIARTYDMALGGYLTKPIRRSDLLQTVSIALGRAHGVSTTSTVRTAASAPIA
                     SRPLHILLVEDSPDNQVLVRSYLNATPYQLDVAEHGGIAVEEFKKRPYDVILMDMNMP
                     VMDGYEATKSIRAWERTHDLPKTQIIALTALVLKEDGEKILEAGCNAHMTKPIKRQTL
                     LEVLQACEGYRPE"
     gene            5294..5620
                     /locus_tag="VFR82_01025"
     CDS             5294..5620
                     /locus_tag="VFR82_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEU4500208.1"
                     /translation="MTSSNGNNQGRVMVAIDPDLQDLVPGFLENRRKDAAALDGALQA
                     KDLRTVRLLGHRMKGDGGGYGFQEISRIGHALEQAAIREDWNTIRQETAGLTTFLAQV
                     DVVYRR"
BASE COUNT         1264 a         1740 c         1632 g         1224 t
ORIGIN      
        1 gccacgatca tggcgagcct cagcttcggc gcgctgtgcg tcgcggggtg gctcttgagt
       61 ctctggcagc atcatgcgct cgcctggatc ggcttcgccg tcatcttcac tctggccgga
      121 gtggcgcgca tgtgttcggc cgtggcctta tcgaaagtcg aagacgtgca tcacacgtct
      181 cataccgacc ccactcctgg tttccggaca ttcctggccg gcaccagcct gagctttcgc
      241 cggtttctcc tcttctccgg tctgatgcac gccgccgtgt tgatcgccgc cccgtttttt
      301 gtcctctaca tgctgcggga cctgcacctc gcctattgga gctatgggac ctggttggcc
      361 gcaggcatct tgggacaact cgtcacatta ggggcatggg gccggttcgc cgatcgattc
      421 ggcaacaaag cgctgctgtc cgtgaccggg ttcatggtgc cgtttttgcc gatgtgctac
      481 ctggccggca cgaatctccc gtttctgttc tgtgtgaact ttctcggcgg cgtgacctgg
      541 ggagggttgg cactgggcct tcaaaactat gtgttcgacg cggtgcggcc ggcggatcgc
      601 gccaaggcgg tcgcgacata cagtacgatc aatgcgctcg gttggtgtgc cggcgccttt
      661 gtgggaagtt ggctggtcaa cctgctgccc tcacagattg ctatagggca gttttctgtg
      721 ccgctggcct caaacctgcc gttggtgttt ttcgtgtcgg gcgtgctgcg gttggcggtg
      781 tcgggcagtc tcctgggagc cttccacgaa gcgcgggccg tagagcatgc gccattcact
      841 cagctggtgt gggagctccc gttgctcaag ccactcgccc aattcctggc gagtccgggg
      901 aagtgaccct tgttatcctg ccctagcgcg gcccctgcga tgaccgctcc cgctcttctt
      961 gcttgagctt atcgaacgtg tgatccgcgt ggccccgcac ttgatcgggt gtcggctgtg
     1021 ccatgggttt gggctcgtgg ctcgagcagc ccgccaccgt caggaacacc cccgctatcg
     1081 ccagcgaaca gaccatgttc ggtaggcttt tccaacactg cacacgaagc gccatataca
     1141 atcctccctc atagaaaagg cagcgcccta cgcacccttg gccggaggca tcatactctc
     1201 ccctgcccgg caaaaccacc agattggcgc caggatcaat tgactgggct ccgatgggcg
     1261 cggtatcctc gaccaaactg ccaccctgca tcgagattcc atgtcattgc gcgatcgtct
     1321 gaccgaagac ctcaaactgg ccatgaaagc gcgcgatcaa ctgcggatgg atgtcatccg
     1381 catgatcaag gccgctgtgc tcaacaagga agtcgagctg aaaaaggatc tcgacgatgc
     1441 cgagatgagc cgcatcatga ccaccatgat caagcagcgg aaagaatcgg tcgagcagtt
     1501 cgaaaagggt cagcgcgctg agcttgccgc gaaggaacga caagaaattt ccatcatcga
     1561 aacttacctg ccccaagccc tctccgccga agaactcgcc caaaccgtcg atgccgtgat
     1621 ccgggagacg ggtgccacgt ccgccaagga catgggactc gtgatgaaag ccgtcatggc
     1681 tcgtctcgcc ggccaaccgg ttgatgggaa gtccgtcagc gacctcgttc gctccaaact
     1741 caagtaagct aatcgtcgca gagtcatctg ctatacttgg tggcgtactg atgggccccc
     1801 gcattaggaa ccaccacgta ggagtttcat gagtattctg atcgttgacg attcaccgga
     1861 tcagcaggct ctcctgcgcg ccatcctgaa caaagccggc cataaagacc tcgttagcgc
     1921 tgatggggca acgagcgcgg gaaaggtgct caacctcaac ggtgattccc ccaccgaaca
     1981 catcgatctt atcctgatgg acgtcctgat gcccggacag gacggcgtcg agacctgccg
     2041 gcaaatcaaa cgctgcgcac acctccaaga cattcccgtc atcatggtga cggccaagag
     2101 tgatctctcg aatctgcaag aggcgtttgc cgccggggcg atggacttta tcagcaaacc
     2161 tgtcagcagc atcgagctcc tcgcgcgcgt ctcctcggct ttgctgctta agcaggaaat
     2221 ggaccgccgg aaaaatcgag aactggaact gcgccgcagc aatgaggaac tgcaaaaagc
     2281 gctgaaggag gtgaaggtgt tacgtggtct cattcctatc tgcgcctcat gcaaaaaggt
     2341 gagaaatgac ggcgggttct ggcagcaatt ggaagagtat cttggggagc attccgaggc
     2401 cgaattcagc catggcctct gtcaaccctg cattaaaaag ctttatcctg gtgtttacca
     2461 ggattagctg ttacgatttc tttactttac cgctacgtcc ctctcaagcc gaggcgccat
     2521 ttccatgagc atattgatcg ttgacgattc tccagacgac cggaccctgc ttcagtcgat
     2581 cctcaccgcc gcgggttatg aacacatcct ggtcgccgaa tcagctgtga ccgcctttca
     2641 tctcctggga ctcgacgggg gcaagcaagg tgccgcccgc gtcgatctca tcctgatgga
     2701 catcatcatg ccggaaatga acggcatcga agcctgccgg cagatcaaag ccgtggagcg
     2761 gttccgcgaa accccgatca tgatggtcac cgtgaaaacc gatccggtgg atttgcagtt
     2821 agcattcgcc gcgggggccc tggattacat cggtaagccc atcaataaga ttgagctctt
     2881 gacgcgcgta cgatcggtgc tgcggctcgt gcacgagatc gagcggcggc gggcgcgcga
     2941 acaggagctg ctggaggtca tgcgccagct tcaggaagct aatcagatgc ttcttcggct
     3001 ctcttctctg gacggcctga ccggcattac aaaccgccgg caattcgacg atttccttga
     3061 tcaagaatgg cggcgtgctg tacgtgaatc gaccccgcta tccctgatca tgctggatat
     3121 cgaccggttc aagatctaca acgataccaa tggccatacg gccggcgacg agtgtctacg
     3181 ccaagttgcc aatgccatct ctggcgctgt caaccggccg ggcgatttgg tcgcccggta
     3241 cggcggtgac gagtttgtcg tggtgcttcc cggcacggcg acggatggag ccgcacaagt
     3301 cgcagagacc ttgaggaagc ggattgaaac gctcggcatt acccactcgg agggcgatca
     3361 catcacgatc agcgtgggat atgccagtat cattcccaat cgaaatgcct ctcccaccga
     3421 tttgatcaag gccgcggacc aagcgcttta tcaagccaaa caggaaggcc gcaaccgggc
     3481 caagcaggcc ggcattctga cactcgttcc ccactcagac gacgagtagc ttggccgcgc
     3541 caccacgcgc cctgaccatg cctcgacctc acgcgccatc tcgaaaaccc cctcgaacct
     3601 cacgccgtcc cacacttgcc cagacacaga cggcacttcg ccgcgaacag gccgcacgcc
     3661 ggcgggaagc gcttcaacac cgccaaaccg agcaggcgct ggtggtggcc aaagaagctg
     3721 cagaagtcgc gaacaaagct aagagtgact ttctggcgag catcagccat gaaatccgta
     3781 caccgatgaa cgcgatcatc ggcatggccg atctgctgtg ggagacagac ctgactccgg
     3841 agcagcgcaa gtacctccgc attttccggc gcgccggcgg cagtctcctg cagttgatca
     3901 acgacattct ggacctttcg aaggtcgaag ccggtcacat agaattggag tcgatcgagt
     3961 tcgatttggg ggatctcgtc gaaaaagcca ttgagatttt ggcgatgcgc gccaatgaaa
     4021 aaggcttgga gctcgcctgc catttggcgc ctgacgttcc ctgttcgttg atcggcgacc
     4081 caaaccggct ccaccaaatt ctcgttaatc tcatcagcaa tgcgatcaaa ttcaccgaga
     4141 ccggatctgt catcgtcgaa gtgacgaatg acgccgatga ggcagggccg ggggccattc
     4201 gtttttcggt caccgacacg ggaatcggca tccctcaaga taagctttct acgatctttg
     4261 aacgcttcac tcaggcccat gcgtccatca gcagacaatt cggtggcacc ggtctcggcc
     4321 tgactatcag taaacagctc gtgggattga tgcacggcac catttgggcc gagagcactc
     4381 ccggacaagg cagcatcttt cactgccgag tcaagtttgg gatcaatccg agcgcaacga
     4441 tacggcctgc ggcctgtcca ctcgatttgc acgacttgcg cacgttggtg gtggacgatc
     4501 acgcgatgaa tcgcaaaatc ctgatggaaa cgctgaagtc atggaatgcc gacgtcacga
     4561 ccgtgagcga tggacaacaa gccttggcgg aattacggca ggcagccctg tcttctcgcc
     4621 cctatggtct tcttctcgtg gactgtcaca tgccgggcat ggatggattc caggtcgttg
     4681 aagcgctccg tcattcggca atggcggagg gagtggcaat catcatgttg gcttccgacc
     4741 attgggcaga tgatatcgcc cggacgtatg acatggcgct tggcggttat ctgacgaaac
     4801 ccattcgccg gtccgacctg ctgcagaccg tgagcattgc actcggtcga gcacacggtg
     4861 tctccacgac gtcgacggtg cgcacggcag cgtcggctcc cattgcttcc agaccgctgc
     4921 acattcttct tgtggaagac tctccagaca accaggttct tgttcgctcc tatttgaatg
     4981 ccacaccgta tcaactcgac gtggccgagc atggtggcat tgccgtcgag gaattcaaga
     5041 aacgaccata tgacgtgatc ctgatggata tgaacatgcc ggtcatggat gggtacgaag
     5101 caaccaagag cattcgagcg tgggaacgga cacatgacct tcccaagacc caaattatcg
     5161 cgctcaccgc tctggttctg aaggaggacg gagaaaaaat acttgaagcc ggttgtaatg
     5221 cgcatatgac gaaacccatc aagcggcaaa cgctgctgga agtccttcaa gcctgtgaag
     5281 gctaccgacc ggaatgacct cctcaaacgg caacaaccaa gggcgtgtga tggtcgcgat
     5341 cgatcctgac ttgcaagatc tggtgccagg attcctcgaa aaccgtcgca aagatgcagc
     5401 agcgctggac ggcgctttgc aagctaagga tcttcgcact gtgcgtctac taggtcaccg
     5461 tatgaaggga gatggcggag gctacgggtt tcaggagatc agcaggatcg gccacgccct
     5521 tgagcaagcc gcaatcagag aagactggaa caccattcgc caggaaaccg ctggtctcac
     5581 cacctttctt gcccaggtcg atgtcgtgta tcggcgctga agccaaccaa aaactttcga
     5641 tgcttagtcg gacggaccct ctcaagtcac gtgccacgtc gtgccgcaac tccgagatgt
     5701 gaacgcgcga acaagacaga ttttctatta gaaccgtgtt cactccttgg agttcattga
     5761 cgtctcccgc aagcaggctc ccttcaacat ttactgcatg accacacccc tccatctagg
     5821 gaccaggaga gctgtccctg acgccatttc ttgaaatact
//
LOCUS       DASTQY010000031         3061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_985645, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000031 DASTQY010000000
VERSION     DASTQY010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985645"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VFR82_01030"
     CDS             complement(<1..218)
                     /locus_tag="VFR82_01030"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEU4500209.1"
                     /translation="MARYVYVAVLVATFLTTALLEAAQKLDAKETAFLQKAAQGQLAD
                     IALGTLALKKASHKDVKEFGAEISEDHQ"
     gene            719..1843
                     /locus_tag="VFR82_01035"
     CDS             719..1843
                     /locus_tag="VFR82_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4500210.1"
                     /translation="MKIAQIAPLWESVPPKLYGGTERIVSYLTEELVRQGHDVTLFAS
                     GDSQTLARLWASSPQAFRSQSGMINHDVPLVLMQEQVFGVDAGRFDIIHSHLDFLGFP
                     LSRRCPTPVLATLHGRLDLPELVPMYREYADMPVVSISNAQRIPLPNVNWQGTVYHGL
                     PDLYNFHPNPGSYLAYLGRICPEKRPDHAIEIAKRVGIPLRMAAKIDPHDREYFEAKI
                     KPLLDHPLVEYLGEVTDAEKCDLLGNAAAVLCTYDWPEPFGIVLIEALACGTPVLAYR
                     CGSIPEIIEDGVTGFICDNLDGMVAAIDRLPLIRRQQCRDSFKTRFTVDRMVQDYLAI
                     YQRMAATVRPMVHVNGRSSRLVDHRRMPSKELVLPHIGSA"
     gene            2319..2639
                     /gene="erpA"
                     /locus_tag="VFR82_01040"
     CDS             2319..2639
                     /gene="erpA"
                     /locus_tag="VFR82_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEU4500211.1"
                     /translation="MITMTPTAEQKIRELLVEEKDTIGLRVFVKGGGCHGYQYGMAFE
                     SQLGEDDTVIEKGEVKVIMDSQSAPLLAGCEVDYQDTVQGSGFAIKNPQAKTTCGCGS
                     SFSA"
     gene            2675..>3061
                     /locus_tag="VFR82_01045"
     CDS             2675..>3061
                     /locus_tag="VFR82_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin oxidoreductase"
                     /protein_id="HEU4500212.1"
                     /translation="MDTVSVIGTKNRKGQVITDPRVMMNEAPRTASFFTGSEVIKEAV
                     RRANVDIMVAYPITPQSEAAALCGELFAEGYIGDYFRGESEFAVMSQCAGSAFGGARV
                     FTTTAGPGTMRAMENFPMWAGARLPIQ"
BASE COUNT          677 a          869 c          842 g          673 t
ORIGIN      
        1 tactggtggt cttcactaat ctcggccccg aattccttca catctttgtg agaagctttc
       61 ttgagtgcta gcgtcccgag tgcaatgtcc gccaactgtc cttgggcagc cttttgcagg
      121 aacgccgtct ccttggcatc cagcttttga gcggcttcaa gcaaagccgt ggtgagaaat
      181 gtggcgacga gtacggcgac atacacgtaa cgagccatgg aacctccggg ggatttagtg
      241 gttggcaact ctctgtacta tggtccagcc agtccatgcc aggaactagg aatcgcctaa
      301 tagtaagcga attgtcactg ggattaatgg cggtcaggcg ctaggaggct gtgttggcgc
      361 ggaagaccgt ttgagaagac acaaaaattt ataacgctgt agaaccatcc gtttataggg
      421 tctggatgaa gcggagctat ggcgcccacc aggctttcac ggtatgatag ctccataagc
      481 agggaaatgt tccgttgagc cacgccgacc gtcccatctc tcgcgcccac tccgctctcg
      541 catcgcgcgt ctgattccgt aggtctacgc tgctgagtcg caacatcgca tttcctccag
      601 gcttgtcttg tgaaaggccg cggcagttca cggacaaaac gcacgacata gttgtcagga
      661 tcctgtcgtg gaccacggct aatcactgag ctcttcgtcc tgaaaagaaa ggccgcacat
      721 gaaaatcgca cagatcgccc ctctttggga aagcgtcccc cccaagctat acggaggcac
      781 ggaacgaatc gtctcttacc tgacggaaga actggttcgc caggggcatg acgtcaccct
      841 ctttgcaagc ggggattctc aaacgctggc ccgcctttgg gcatccagcc ctcaggcgtt
      901 tcgatcgcaa tctgggatga tcaatcatga tgtgccactt gtactcatgc aggagcaggt
      961 cttcggcgta gacgccggca gattcgatat tattcattcc catctcgact ttttgggctt
     1021 ccccctgagt cgccgttgcc ccaccccggt gctcgcgacg ttacatggac gattggatct
     1081 tcccgagtta gtacccatgt acagagagta tgccgacatg ccggttgtgt cgatttcaaa
     1141 cgcgcagcgc atacccttgc cgaacgtcaa ctggcagggc accgtgtatc acgggctgcc
     1201 tgacctctac aattttcatc ctaatcctgg atcctatctc gcctatcttg gaagaatctg
     1261 tccggagaaa cggcccgatc acgcgataga aattgccaaa cgggttggca ttccgctccg
     1321 aatggccgcc aagatcgatc cacacgatcg tgagtacttc gaagcgaaga ttaagccgct
     1381 gttggaccat ccgctcgttg aatacctcgg tgaagtcact gatgcagaaa agtgcgattt
     1441 gctcgggaac gcggctgcag tcctgtgcac atatgactgg ccggaaccgt tcgggatcgt
     1501 gttgatcgag gccttggctt gcggaacgcc tgtgttggcc tatcgttgcg ggtcaatccc
     1561 cgagatcatc gaggacggtg tcacaggatt tatttgtgac aatctggatg ggatggtggc
     1621 cgcgattgac cggctgccgc tcattcgacg ccaacaatgt cgggactcat tcaagacccg
     1681 tttcactgtg gaccgcatgg tccaagacta tctcgccatc tatcaacgaa tggccgcgac
     1741 cgtgcgacca atggtccacg tgaatgggcg atcatcgcgt ctggtagatc acaggcgaat
     1801 gccatcaaag gaactcgtct tgcctcacat cggtagcgcg tgacctatgt gcaacagcac
     1861 aggctatcaa gagaacagca ctcgagcatg agggagccta ttaggatcaa gtggtagttg
     1921 gcatctcgcg ttacaccctg tgatgtcaga gatggcgcct ggtcaatcag ctacagcagc
     1981 acacccgcgc tggatggatg gcgaccgccg cataaaaggt ccgtttgagc gctccactac
     2041 cccttaatag cgcttgactc cggtccctat agcgcctagc ctgatctaag acgtataaag
     2101 ccacgccgac tgtcctatcc ttcgcgcgtc acacttcctt cctgcgcgat tgattccccg
     2161 gtcctcgccg cttcctacgc caacgttttt tcaaggcatc tctaagtcat gtgcatgcgg
     2221 gcggctaccg cctgcgtttg aaaaggtatg ccttctcgag tcggtcaatg tgtggtgtac
     2281 gcgcccaagg tccgccaacg acaataagga gagatgccat gatcaccatg acgccaacgg
     2341 ctgaacaaaa gattcgggaa ctcctcgtgg aagagaaaga tacgatcggc ctgcgcgtct
     2401 tcgtcaaagg cggcggctgc catggctacc aatacggcat ggccttcgag tctcagctcg
     2461 gagaggacga tacggtcatc gaaaagggcg aagtcaaagt cattatggat tcgcaaagcg
     2521 ctccgctgct ggccggctgc gaagtcgatt atcaagacac cgtgcaaggg tccggctttg
     2581 ccatcaaaaa tccgcaggcg aaaaccacgt gtgggtgcgg cagttcattc agcgcatgac
     2641 tcgatggtgc gcaggaagct agggaggttg atccatggat accgtatcgg tcatcggaac
     2701 gaaaaataga aaaggccagg tcatcaccga cccccgcgtc atgatgaacg aggcgcctcg
     2761 cacggcgtcg ttcttcaccg gcagcgaagt catcaaggaa gccgtcaggc gcgccaacgt
     2821 cgatattatg gtggcctatc ccattacgcc ccagagcgaa gcggccgctc tttgcggcga
     2881 gctctttgcc gagggctata tcggcgacta cttccgcggc gagagtgaat ttgcggtcat
     2941 gtcgcaatgc gccggctccg catttggcgg ggcccgcgtg ttcacgacaa ccgccggccc
     3001 tggcaccatg cgcgccatgg agaatttccc gatgtgggcc ggcgcgcgat tgccgatcca
     3061 a
//
LOCUS       DASTQY010000032         6899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1590079, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000032 DASTQY010000000
VERSION     DASTQY010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590079"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VFR82_01050"
     CDS             <1..586
                     /locus_tag="VFR82_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEU4500213.1"
                     /translation="AAETSIPPHTDIMCLLQAERARKSLLTRPPAAPTEAQSTGPAFA
                     EPDAKLLIIIHEESVMDNQTNERRPEVLRTLLIGRRSTLQREIDHLIAQHRDNHTHFR
                     DNSVLDVEDMSLRDATGAQQIALLEARTQERIQLDEALRRLDEGTYGICEDCEAPISP
                     ARLRALPFARRCVGCQEQFELFQNIVRREDREEI"
     gene            597..1142
                     /locus_tag="VFR82_01055"
     CDS             597..1142
                     /locus_tag="VFR82_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU4500214.1"
                     /translation="MREAKSRHDDMEDRNMDEEDEASADLFEMLKTDHRHVQDLFTRF
                     EDADKRARSTIAEEALTALEVHAALEEELVYPAIAEVVEEEELVNEAKEEHHVAKLLI
                     KELHKMDAGDEEFATKFKVLGEIVGHHIEEEEGEMFPQAQEGGFEPETIAQEVVKRRE
                     KLMQKYQGGKKSSASKRKKAA"
     gene            1248..1721
                     /locus_tag="VFR82_01060"
     CDS             1248..1721
                     /locus_tag="VFR82_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500215.1"
                     /translation="MHVGDMLLWGLLATGLLTTVLRAGQALHLTRMDTALVLGTMVTG
                     DRDRAKIIGFVIHFFNGWWIATIYVLFFHAFGMATWWLGALMGIAHTAFILVAVLPSL
                     PGVHPRMASDLTGPSPTRMLEPPGFMALNYGFRTPLAVLIAHIAYGTVLGAFYQV"
     gene            complement(1766..3559)
                     /locus_tag="VFR82_01065"
     CDS             complement(1766..3559)
                     /locus_tag="VFR82_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU4500216.1"
                     /translation="MTAYPPIHDYALIGDCRTSALISSSGSIDWMCLPRFDSPSCFNR
                     LLDWEHGGYCDILPTAPFRTRRFYQLNTAVLITEFRTSDGLAQLTDLMPVTADGERAS
                     VPHPSRQVLRRVEVLEGTVEFSLTVKPRPHDGRTIPCFESRGGAGYCADFGGAVLFVA
                     VDSPMDIRNGALTGRVVMDRGESLTLWMAYEEEAPAVYPRLSDAKTAIADTQAYWTGW
                     AEHCQYAGPFKDQALRSAITLKLLSYAPSGAIVAAPTTSLPERIGGDLNWDYRYCWLR
                     DASFTAHAFFRLGFHAEALAFMQWITHATTLTYPRLQVMYTLHGEASIPERTWPFFEG
                     YRGSQPVRFGNAASQQHQLDVYGEVLDALSLSVEAGYPVDRDTRRWIVDVGNLLSASW
                     PYPDHGIWEVRGEPRHYVHSKVMCWTALDRIERLTKKLGLRASLGPWVQARDALTRTV
                     LKTGYSKTRRSFVQVLGGTRLDAAALTFGLVGFIDGRDPLMTSTILAIQRWLTRGPLV
                     YRYVAEQATGREEGAFLPCSFWLVEALALAGRMGEAEDLTYRLNAHGNDLGLFPEEIR
                     HQDGAFLGNFPLALTHVAHLSALLRIAGKET"
     gene            complement(3837..4208)
                     /locus_tag="VFR82_01070"
     CDS             complement(3837..4208)
                     /locus_tag="VFR82_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU4500217.1"
                     /translation="MPTAMIQDPAKAKEFFEAKMAFTTGPVELERMMKEGSVNVVDVR
                     AADDYAKGHIPGAVNLPKERWASLQGLSKDRVNVVYCYSIVCHLGATAAVEFAARGFS
                     VMELDGGWRWWKDDGFPIETS"
     gene            complement(4305..4634)
                     /locus_tag="VFR82_01075"
     CDS             complement(4305..4634)
                     /locus_tag="VFR82_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500218.1"
                     /translation="MHGRTASLAVILLLGGCAGGGQQFGDAGTASPQQEAASQRTDYR
                     TQANALREMADRRQLEADLLARELGASDPSVEKKRRLAEELRAAAEDADNKARSLSRN
                     VPHGMVQ"
     gene            complement(4656..5786)
                     /locus_tag="VFR82_01080"
     CDS             complement(4656..5786)
                     /locus_tag="VFR82_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU4500219.1"
                     /translation="MQPSLRLGRLAGIEIGIHYTWLLIAVLITGSFFAYFSSTYAHWP
                     LRTTWSAAIVISVLFFASIVLHELAHALVAKAYGMQVRTITLFALGGVAHIEKQAATA
                     TAEFWMGLAGPVMSAAVGLLCGWVGSWLGAPAKGIGFTPLAAVLISLSSLNLMLAMFN
                     LIPGFPLDGGRILRAIIWGLTGDENRATRIASRAGQGVALSLFGLGLWEFLGAESLSG
                     LWLGLLGWFLLDAATASYVQAEVLTGLRGMRVSDLMSNDCLTVPGDVPLQRFADEYVL
                     KTGRRCYAVEDRDTIIGLITSADLAKVENSRWTQIPVMQVAHPLSEVRSVSPDTPVAE
                     ALQTMGREDVNQLPVVEAGRMEGMLSRSHILRALETRSELSM"
     gene            complement(5838..6317)
                     /locus_tag="VFR82_01085"
     CDS             complement(5838..6317)
                     /locus_tag="VFR82_01085"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU4500220.1"
                     /translation="MATCRLPREDAAMLDSGYLNLFGRVLLGLIFLISGINKIADPQG
                     TQHYMALMGMTTMTTLFYIGAVAIEIAGSLSLLLGYRTKTGAWLLFVFMIPTTLVFHT
                     NFADPNQMIHFLKNLAMMGGLLYVAEYGGGRYSMDAGLERVERIEEPRRPLRKVVHQ"
     gene            complement(6326..>6899)
                     /locus_tag="VFR82_01090"
     CDS             complement(6326..>6899)
                     /locus_tag="VFR82_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500221.1"
                     /translation="GKARKGNCSIDVIFSSGNGVCTVDDAWFRHAMPAEILGIQVEVV
                     PAEEMLWSKAFVMERNRYDGGDIAHLLRARAPRLDWARLLFRFGVYWRVLFSHVVLFG
                     FVYPEQRLRIPDWFMHEMIRRLKLDMQCLPSDRRVCQGTLLSWSQYLVDMDRGDYEDA
                     RQMPRGRLSSEETQYMTDVLQREEKDAHER"
BASE COUNT         1544 a         2047 c         1906 g         1402 t
ORIGIN      
        1 cgctgcggag acaagtattc cgcctcacac agacatcatg tgtctgctcc aggcggagcg
       61 cgcaaggaag tccctgctaa cccgcccacc ggcagcgccg acggaagcac agagcactgg
      121 ccctgctttt gcagaaccgg atgcgaagct gcttatcatt attcacgagg agtctgtcat
      181 ggataatcag accaacgaga ggcgtccgga agtgctgcgc accttgctga tcggacggcg
      241 ttcgacgctc cagcgagaaa tcgatcacct gatcgctcag caccgtgaca atcacacgca
      301 ctttcgcgac aactctgtcc tcgacgtcga agacatgtcc ttgcgcgatg cgaccggggc
      361 tcaacagatt gccttgctgg aagcacgtac acaggaacgg attcagttgg acgaagccct
      421 gcgcaggctc gatgagggga cgtacgggat ctgcgaggat tgcgaggccc ctatctctcc
      481 ggcacgttta cgcgctctgc cttttgcgag acgatgcgtc ggttgccagg agcagttcga
      541 gctgttccaa aacatcgtac gccgcgaaga ccgtgaagaa atttaaagga ggcaccatgc
      601 gggaagccaa atcaagacac gacgatatgg aagatcggaa catggatgag gaagatgaag
      661 cttccgccga tctctttgag atgttgaaga cagatcatcg tcacgtgcag gacctcttta
      721 ctcgattcga ggacgcagac aaacgagccc gctccacgat cgccgaggaa gctcttacgg
      781 cgctcgaggt gcacgcggca ttggaagaag agcttgtcta tccggcgatt gcggaagtcg
      841 tggaggaaga ggaattagtc aacgaggcaa aggaagagca tcacgtggcg aaactcctca
      901 tcaaagaact gcataagatg gacgcgggag acgaggaatt tgcgaccaaa ttcaaagtgc
      961 ttggcgagat tgtcggtcat catatcgagg aagaagaggg cgaaatgttc ccccaggccc
     1021 aggaaggtgg tttcgagccg gagactatcg cgcaagaagt tgtgaagcgt agagaaaagc
     1081 tgatgcagaa atatcaaggg ggaaagaaat cgtcggcctc caagcgaaaa aaggctgcat
     1141 agagcgaacc ccggtcgtcg ggtctgtggt ctcgggtgcc tgagtgatac agggctaatc
     1201 cgagccgtgg gcccggcgtg accggccagg gttcagcagg acatatcatg catgtcggtg
     1261 acatgttgct gtggggactg ctggcgacgg gcctgctgac cactgtgctt cgggcgggcc
     1321 aagcactcca ccttacccgt atggataccg ccctggtgct cgggaccatg gtcaccgggg
     1381 atcgtgatcg ggccaagatc atcggcttcg tcatccattt cttcaacggg tggtggatcg
     1441 cgacgatcta tgtgctcttt tttcatgcct tcggcatggc cacctggtgg cttggagcgc
     1501 tgatgggtat cgcccacacg gcattcatcc tggtagccgt cctgccttcc cttccaggag
     1561 tccatccgcg tatggcttcg gatttgacag gcccaagccc tacgaggatg ctagagcccc
     1621 caggctttat ggccctgaac tacgggttcc gcactcccct cgccgtcctt attgcccaca
     1681 tcgcctatgg caccgtcctc ggtgcgtttt accaggtgta acagcagatg aagcctcccg
     1741 aatggtccgg tccgctttac tcgcctcatg tctctttgcc ggcaatgcgc aggagtgcac
     1801 tgagatgggc cacatgggtt aaggccagag ggaagtttcc gagaaacgca ccgtcctgat
     1861 gacggatctc ttccggaaac agccccagat cgttgccatg agcattcagc cggtacgtta
     1921 aatcttcagc ctcacccatc cgtccggcaa gggcaagggc ctccaccaac caaaaactgc
     1981 aaggcagaaa cgctccctcc tcgcgcccgg ttgcctgctc agccacatac cggtaaacca
     2041 aaggcccgcg tgtgagccag cgttgaatcg ctagaatcgt tgatgtcatc agcggatcgc
     2101 gtccatcgat aaagccgaca agtccgaagg tcagagctgc ggcatccaag cgtgtcccgc
     2161 ccaagacctg aacaaaactc cgccgcgttt tcgagtagcc ggttttgagc accgtgcggg
     2221 tcagtgcgtc gcgtgcctgg acccaggggc caagggaggc gcgcaggccg agtttcttcg
     2281 tcagacgctc gatgcgatcg agtgcggtcc agcacatgac tttcgagtgc acataatgcc
     2341 ggggctctcc tcgcacttcc cagattccat gatcaggata cggccatgaa gcagaaagga
     2401 gattgccgac gtcgacaatc cagcgtctgg tatcccggtc cactggatag ccggcctcaa
     2461 ccgataggga taaggcatca agcacttcgc cgtacacatc gagctgatgt tgctgtgagg
     2521 cggcattccc aaagcggaca ggctgtgatc ctcgatagcc ttcaaagaac ggccacgtgc
     2581 gttccggaat tgaggcttcc ccatggagag tgtacatgac ttgcagtcga ggataggtca
     2641 gcgtggtcgc atgcgtaatc cattgcatga aggccaaggc ttcggcgtga aatcccagtc
     2701 gaaaaaatgc gtgtgcagtg aatgaagcat cccgcaacca gcagtaccga tagtcccagt
     2761 tcaaatcgcc gccaattcgc tcgggaagcg atgtggtggg cgccgccaca attgctccgg
     2821 acggcgcata gctcaacagc ttcagggtga tagcgcttcg gagagcctga tccttgaaag
     2881 gaccggcata ctgacagtgc tctgcccacc cggtccaata cgcctgtgta tccgcgatgg
     2941 ctgttttcgc gtcggatagt cgaggataca ctgctggcgc ctcctcttcg taggccatcc
     3001 agagggtgag actttctcct cgatccatga ccacgcggcc ggtcagggct ccatttcgga
     3061 tgtccatcgg actgtccaca gccacgaaca gcaccgctcc tccaaaatcg gcacaatacc
     3121 ccgcaccccc ccgagactcg aagcaaggga ttgtccggcc gtcgtgtggt ctcggtttca
     3181 cggtcaggga aaattccacc gtgccttcca acacttcaac acgacgcagg acctgccgcg
     3241 atgggtgcgg aacagatgct cgctccccat cggcagtcac tggcatgagg tcggtcaact
     3301 gggcgaggcc atcggatgtt cgaaattccg tgatgaggac tgccgtattc agctgataaa
     3361 aacgtcgggt ccgaaaaggt gcggtgggaa ggatgtcgca gtagccgcca tgctcccaat
     3421 ccagcaggcg gttgaaacaa gatggactgt cgaagcgagg aagacacatc cagtcaatgg
     3481 atccgctact ggagatgagc gcactagtcc ggcagtcccc gatgagcgcg tagtcgtgga
     3541 tcggaggata ggccgtcaag gtagggcctc agatatcacg aactttccaa ttccgatgtg
     3601 acatccacac ttgcaaatac ggatcgatga acagacggag tacgtaacgg acagacattc
     3661 ctttaacggc cgggtcgatc ggaatcccgg atgcctggtg tggggttccg cggacggatg
     3721 atggccgtat ccagccggca ttatccacgt tcttgaacgc tttgcccgcc tggagacgaa
     3781 cagcagagac ctaagaacct gctctcttcg ggcgcttgga aacccgagcg tgcgcgctat
     3841 gatgtttcga tggggaaacc gtcgtctttc caccatcgcc agccgccatc caactccatt
     3901 acagagaatc cgcgggcagc aaattctacg gcggcagttg ctccgagatg acagacgatc
     3961 gaataacaat acaccacgtt cacgcgatcc ttgcttaagc cctgaaggct cgcccaacgt
     4021 tctttcggca ggttcacagc tccaggaatg tgtccttttg cataatcatc ggccgctcga
     4081 acgtcgacca cgttgacgct gccttccttc atcatccgct ccaactctac tgggccggtg
     4141 gtgaaggcca ttttggcctc gaaaaactct ttggcttttg caggatcctg tatcattgcc
     4201 gttggcatgt cgcctccttc ttactgagcc ggatgccata actcatcagc atcccttcca
     4261 attgtgatgc ggtgcggatt tcaagggcag cacagtggca atggctattg gaccattccg
     4321 tgaggcacat tgcggctgag actgcgtgcc ttgttgtcgg cgtcctccgc ggccgctcga
     4381 agctcttccg ctaaccgacg ctttttctcg acactgggat cgctggcacc gagttcccgg
     4441 gctaagaggt cggcctcgag ctgccgccgg tcggccattt cgcgcaacgc gtttgcctgc
     4501 gttcggtaat cagtgcgctg ggacgctgcc tcttgctgcg gagatgccgt tcctgcatct
     4561 ccgaactgtt gcccgccgcc tgcgcaacct cccaacagga gaatgaccgc taatgaagct
     4621 gttcgtccat gcatgctgac ctccctgtgg ctgcgttaca tactcaattc acttcgggtc
     4681 tccagcgccc gcaagatatg actgcgcgac aacatacctt ccattcgtcc ggcttcaacc
     4741 actggcagtt ggttaacgtc ctcccgtccc atggtttgta acgcctctgc gaccggagta
     4801 tcgggagaca ccgaccgaac ttcactcagg ggatgcgcca cttgcatcac cggaatctgt
     4861 gtccacctag aattttcaac tttcgccaga tccgcagacg tgatcaatcc gatgatcgta
     4921 tctcggtcct ctacggcata acaacgccgg cctgtcttaa gcacatactc atcggcaaac
     4981 cgctgcagag gcacgtcacc gggcaccgtc agacaatcgt ttgacatcag gtcgctgacc
     5041 cgcatccctc ggaggccggt cagaacctcc gcctgtacgt aactggcggt cgcggcgtca
     5101 agcaggaacc atcccagcaa tccaagccag agaccgctca gactctctgc tccgaggaat
     5161 tcccataatc cgagcccgaa gaggctcaac gccacgccct gtccggcacg tgaggcgatt
     5221 cgagtggcgc gattctcgtc tcccgtgagt ccccaaatga ttgctcgtaa tattctgccg
     5281 ccgtctagtg ggaaccccgg aatcaagttg aacatggcta acatcaagtt caatgaactc
     5341 aacgagatca atactgccgc caacggcgta aatccaattc ccttcgctgg tgcacccaac
     5401 caggacccca cccatccgca cagcaggccg accgccgcac tcatgacggg gccggccagt
     5461 cccatccaaa actcagcagt agcggtggcg gcttgcttct cgatgtgcgc gaccccgccc
     5521 aacgcaaaaa gcgtgatcgt gcgcacctgc atcccatatg ctttcgctac gagggcgtga
     5581 gccagctcat gcaggacgat cgaggcgaaa aaaagcacgc tgatcacgat ggcagccgac
     5641 caggtcgttc ggagcggcca atgcgcatag gtactggaaa agtacgcaaa gaacgagccc
     5701 gtgatcagca ccgcaatcag cagccacgta tagtggatgc cgatctctat gccagccagg
     5761 cggccgagtc gtaatgacgg ttgcacgcgc gtgtcctcgt ttaccttcct gaggtagctt
     5821 ctccttgggc taccttctta ttggtgcacg actttcctca atgggcgacg cggctcttct
     5881 attcgctcta cacgttccag gccggcatcc atgctatagc ggcctccccc gtactcggcg
     5941 acgtataaca ggccgcccat catggcaaga ttcttcaaga agtggatcat ctggttcgga
     6001 tctgcgaagt tggtatgaaa gaccagtgtg gtgggaatca taaatacaaa caggagccac
     6061 gcgccggtct tcgtacggta ccccaggagg agagagaggg aacccgcgat ctcaatggca
     6121 accgcaccta tgtaaaagag ggtagtcatc gtcgtcatgc ccatcaaagc catgtagtgt
     6181 tgagtccctt ggggatcggc aattttgttg atgcctgaga tgaggaagat gaggccgaga
     6241 agcacccggc cgaacaaatt aagataaccg gaatcgagca tggcagcatc ctcccggggc
     6301 agacggcagg tggccatcgg cccacctaac gttcgtgtgc gtctttttct tcgcgctgca
     6361 acacgtcggt catgtattga gtctcctccg atgacagtcg tccccttggc atctgcctgg
     6421 cgtcctcgta atctccacgg tccatatcca ccagatactg cgaccacgac agcagtgtgc
     6481 cctgacagac tcgccggtcc gaaggcaggc attgcatgtc caatttcagc cggcggatca
     6541 tttcatgcat gaaccagtcg ggtatgcgca accgttgctc gggatacacg aagccgaaga
     6601 gcaccacatg gctgaacagt accctccagt agacaccgaa acggaacagc agccgagccc
     6661 aatccaacct cggcgcacgg gcgcgcaaca aatgtgcaat atcgccgccg tcgtatcggt
     6721 tgcgttccat cacgaacgcc tttgaccaca gcatttcctc cgcgggcacg acttcgacct
     6781 gtataccgag aatctcggcg ggcatcgcat gccggaacca tgcgtcgtct accgtgcaca
     6841 cgccattgcc ggagctgaag atgacatcga tactgcaatt gcccttgcgg gccttgcca
//
LOCUS       DASTQY010000033         5572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_582311, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000033 DASTQY010000000
VERSION     DASTQY010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582311"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VFR82_01095"
     CDS             complement(<1..440)
                     /locus_tag="VFR82_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500222.1"
                     /translation="MKFLLYTLLVVFLVPLQATLLPHLAIWDVRPDLGLVVAALIGVL
                     AGELDGLLVGLAIGWVLNLYSAGDLWLNLLTTGGTGLFAGLLARHVAEVTPTVLSVAV
                     LVLSLAGGLVAVLGMKHGTISDTWWMVQSVVVPQACFDAAVGAAL"
     gene            complement(437..1312)
                     /gene="mreC"
                     /locus_tag="VFR82_01100"
     CDS             complement(437..1312)
                     /gene="mreC"
                     /locus_tag="VFR82_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU4500223.1"
                     /translation="MVNVRSPYGARRLALGLFALLLAALFLFPGQSQGLLEYLDGPVG
                     QLVRLPLEAVSSLDQGISAMWQQYVSLQNVADDNRRLRQEMDWLRGENHQLREAAAAT
                     QRLTVLLQFKDQASSTMVAAQVIGRDSSNRYRSMILNKGESDGIQKDMGVVTPSGVVG
                     RVVKTTGATSVVLLVTDPNNAIAGLIQRTRDEGIVEGTPQGRAKLKYIPMLSSVKEGD
                     QVVTSGLVGGFPRGLAIGTITAIDREEGALFQTAELAPEVDVNHLEEVLVIQTPHGES
                     ADAVRKLDGGTKRQP"
     gene            complement(1336..2385)
                     /locus_tag="VFR82_01105"
     CDS             complement(1336..2385)
                     /locus_tag="VFR82_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU4500224.1"
                     /translation="MGFTSDVFGWFSNDLAIDLGTATTLVYVHGKGIVLNEPSVVAVE
                     KKTEKVLAVGADAKKMLGRTPGNIVAVRPMKEGVIADFEMAEQMLKRFIQKAHNRSAF
                     VRPRIIIGVPSRITQVEQRAVRDSAELAGAREVYLIEEPVAAAIGAGLPITEPSGNMV
                     VDVGGGTTDIAVISLGGIVYSESVKVAGDRMDDSIMNYIKKKYNLLIGEHMAERIKFE
                     IGSAYPFEERKTMMIKGRDLISGIPRTLVVDDAEIREALQEPIGTIVNAIKVALENTP
                     PELAGDIIDRGIVLTGGGSLLKGMDTRFREETNLPIITVDDPLTSVVLGVGKILDELD
                     LLAKVAVMSQANTFR"
     gene            complement(2428..2883)
                     /locus_tag="VFR82_01110"
     CDS             complement(2428..2883)
                     /locus_tag="VFR82_01110"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEU4500225.1"
                     /translation="MTEQVAFHPLGAGIYPKATVLNRFIAKLIDFFLAAAAAEVIVPV
                     GLLAGVAYLFVADGFAGGRSIGKRLIGLQTVLPDSREPAGFRESIIRNIPFAAAQLLF
                     AVPYVGWVASTVIVAFEAILIIGNEQGRRLGDELAGTQVLDAGRLALPD"
     gene            3059..3757
                     /locus_tag="VFR82_01115"
     CDS             3059..3757
                     /locus_tag="VFR82_01115"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEU4500226.1"
                     /translation="MIKILRFANEKYPWFLAITIGFIAVTFIVGMGWWGFGEQSGSVV
                     ATVGDLPVSLDEFKRAYENTYRFYKEKVPGEFKDETIKQYVVEQLVENRVWLVAAHNM
                     GLTVADEELRESIMQIPEFQNNGKFDPERYQRLLAANHLTPSIFESMQARELLSVKAR
                     VIVSNAVSLTPSELAEAQSLMLRQTDPDPAKAAAAKERTIQDVLFQKQQRALMAYAQS
                     LKATLPIAIHRELL"
     gene            complement(3735..4790)
                     /gene="queA"
                     /locus_tag="VFR82_01120"
     CDS             complement(3735..4790)
                     /gene="queA"
                     /locus_tag="VFR82_01120"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU4500227.1"
                     /translation="MQLSEFDFPFDPSLVATEPTLPREQAKLLVLNPINRVIMHRRIA
                     DLADLLDPGDLLVVNDTRVRPARVTGRKRASGRPVEMLFVKPVADREWEVLMKGTWRA
                     GQTIEIDGESDVTVLSREGGRTVVRIDGTLPIPEWFRMHGRMPLPPYIKRPPTDQDRD
                     WYQTVFARDEGAIAAPTASLHFTPALLSRLTDRHIELATITLHVGVGTFKPVTAVRIE
                     EHRMEAEWVEVGPATVRAIERVKAVGRKVVAVGTTVVRAIETAALRGTLQPYRGETSL
                     FVRPGFEFKVVDALLTNFHLPQTTLLMLVSAFTGTGFLRRAYEEAVQTRYRFYSYGDA
                     MLITDRGERRARAVPGG"
     gene            complement(4823..>5572)
                     /locus_tag="VFR82_01125"
     CDS             complement(4823..>5572)
                     /locus_tag="VFR82_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEU4500228.1"
                     /translation="VNSSWHPEMLKAQAVAARTYALYQQMLSASRDYDVVATVQDQVY
                     KGKDGVDTAVLRAVDETRGLVLTYQQAPIYAAFSSTAAGLTEDAVNVWSKDYPYLKGV
                     ECPFDLDSPFYQWKASFRMDKLEQNLRQQGYAVGTITSIEPLAFSRGGRVAKLRILHS
                     EGELVLRGEDLRKAVGYSIVPSTQFTIDSVGREVVLSGYGAGHAVGMCQWGAKELAEL
                     GYPFSTILSYYYPGTELQEMSLTKPPIPPTS"
BASE COUNT         1239 a         1711 c         1503 g         1119 t
ORIGIN      
        1 agcgcggcac ccacggccgc atcaaagcag gcttggggaa cgacgaccga ctgcaccatc
       61 caccaggtat cggatattgt cccatgcttc atgccgagga cggcgacgag gcctccggcc
      121 aacgacaaga ccaacacagc cactgaaagc accgtcgggg tcacttcagc cacgtgtctt
      181 gccaagagac ccgcaaacaa cccggtgcct cctgtcgtga gcaaattcaa ccagagatcc
      241 cccgcagaat acaaattcag cacccaccca atggccaaac ccacgagcaa tccatccaat
      301 tcgccggcta atactccgat tagggcggcg acgacaagac cgagatccgg cctgacgtcc
      361 catatggcca agtggggaag caacgtcgcc tgtagcggca cgaggaacac gaccaacagg
      421 gtgtagagta agaacttcat ggctgccgtt tcgttccgcc atccagtttc cggacagcat
      481 ccgcggactc gccgtgtggc gtttgaatca ccaacacttc ttccagatgg ttgacgtcga
      541 cttccggggc gagttcggcc gtctgaaaca gtgcgccttc ttctcgatcg atcgcggtaa
      601 tcgtcccgat cgccaacccg cgtggaaagc ctccgaccaa gccggaggtg acgacctgat
      661 ctccttcctt caccgacgac agcatgggaa tgtatttcag cttggcccgt ccttgcggcg
      721 ttccttcgac gatgccttcg tctctggtgc gctggatcaa tccggcgatg gcattgttgg
      781 gatccgtcac aagtaagacg accgacgtgg cacccgtcgt cttcacgacc cgccccacga
      841 caccggaagg cgtcaccact cccatatctt tctggatgcc atcgctttct cccttgttca
      901 ggatcatgga gcgataacgg ttactggaat cacgaccaat aacctgcgca gcgaccatcg
      961 tggatgacgc ctggtctttg aactggagca gaaccgtgag ccgctgcgtt gcggcagccg
     1021 cctcacgcaa ttgatgattc tcaccccgca gccaatccat ctcctgccgc aatcggcgat
     1081 tgtcatcagc cacattctgg agtgaaacat actgttgcca catcgccgaa atgccctgat
     1141 ccagagaaga cactgcttca agaggcagcc ggacgagttg gccgaccggc ccgtccaggt
     1201 attcgagaag cccctgactt tgtccgggaa agaggaagag agctgccagg aggagggcaa
     1261 acaatcctag cgccagacgc ctagcaccgt agggagaccg cacgttgacc atccacatgt
     1321 accgggatgg agcctttacc ggaaggtatt cgcttgagac atgactgcga ccttggccaa
     1381 tagatccaac tcatcgagga tcttgcccac tcccaacacg acggaagtca ggggatcgtc
     1441 gacggtgatg atcggcagat tcgtttcttc ccggaagcgg gtatccatgc ccttgagcag
     1501 agagccgccg ccggtcagca cgatgccacg gtcgatgatg tcacccgcca actcaggcgg
     1561 ggtattctcc agggctacct tgatcgcatt gacgatcgtt ccgatcggct cctgcagcgc
     1621 ctctctgatt tccgcatcat ccaccaccaa tgtgcgcggg atgccggaaa tcaggtcgcg
     1681 ccctttaatc atcatcgttt tacgctcctc gaacgggtag gcggagccga tctcgaactt
     1741 aatccgttcc gccatatgct cgccgatgag gaggttatac ttctttttga tgtagttcat
     1801 gatcgagtcg tccatccggt caccggcgac tttcactgac tcgctgtaga cgatgccgcc
     1861 cagcgagatg accgcgatgt cagtcgtacc tccgcccacg tccacgacca tgttaccgga
     1921 cggttcggtg atggggagac ccgcgccgat ggccgccgcc accggttctt cgatcaaata
     1981 cacttccctc gcgccggcca gttcggccga atcgcgcacc gctcgttgct ccacctgggt
     2041 gatccgggac ggcacgccga taatgatgcg aggtcggacg aaggcgctac gattgtgcgc
     2101 tttctgaatg aaccgcttca acatctgttc ggccatttca aaatccgcga tgacgccttc
     2161 cttcatcggg cgtaccgcca cgatatttcc cggggtgcgg ccgagcatct ttttcgcatc
     2221 ggcaccaacg gcgagaacct tttccgtctt tttctcgacg gcaacaacgg agggttcgtt
     2281 caagacgatg cccttcccat gcacgtacac caacgtcgtg gctgtgccca ggtcgatggc
     2341 caggtcgttc gagaaccagc cgaacacgtc actcgtaaaa cccacgatca ctctcccttc
     2401 acatccaaac cgaatcctgg gatcctgcta gtcgggcaac gccaatcggc ccgcatccaa
     2461 cacctgggtc cccgccagtt catcaccgag ccggcggccc tgctcgtttc caatgatcaa
     2521 gatcgcttcg aacgcgacaa tcaccgtcga ggccacccag ccgacataag ggacggcgaa
     2581 caatagctgc gccgccgcga aaggaatatt gcgaatgatc gactctcgaa atcccgccgg
     2641 ttcacggcta tcgggaagaa ccgtctgaag gccgatcaac cgcttaccga tgctccgacc
     2701 ccccgcaaat ccatcggcca cgaagagata ggcgactcct gcgagcaacc cgacaggcac
     2761 aatgacttcg gcggcggcgg ctgcgagaaa gaagtcaatg agcttggcga tgaaccgatt
     2821 gagaaccgtc gccttggggt agatccctgc acccaatgga tggaacgcca cctgctccgt
     2881 caaggatctc ctcgtaatta ggggcaaagt ataacaaaga ccaggaccta gcacaaacga
     2941 atcaccattc tttgacgcgg acgacgacga ggcccactgg ccctgaaaag cttgccttcg
     3001 gcaggtgagg ctgggtacaa tccgcgacta tttcacttgg cgcgagggat ccgggtccat
     3061 gatcaaaata ttgcggttcg cgaacgaaaa atatccctgg tttctggcga tcacgatcgg
     3121 atttatcgcc gtgacgttca tcgtgggtat gggttggtgg ggcttcgggg agcaatccgg
     3181 ctcggtcgtc gcaaccgtcg gcgatctgcc cgtctcattg gatgaattca agcgcgcgta
     3241 tgaaaacacc taccggtttt acaaggaaaa ggtgccggga gagttcaagg acgaaacgat
     3301 caagcaatat gtggtcgaac aactggtgga gaaccgggtg tggttggttg ccgcgcacaa
     3361 catggggttg acggtagccg atgaagagct gcgcgaatcg atcatgcaaa ttcccgaatt
     3421 tcagaacaac ggcaaatttg atcctgaacg gtaccaacga ttgctggctg ccaatcattt
     3481 gacaccctca atctttgagt cgatgcaggc aagagaactg ctgagcgtga aggcacgtgt
     3541 gatcgtcagc aacgccgtct ccttgacgcc atccgagctt gcggaagcac aatccctcat
     3601 gctgaggcaa acggatcccg atccggccaa agccgctgcg gcaaaagaac gcaccattca
     3661 agacgtgttg tttcagaagc aacagcgcgc gctgatggcc tatgcccaat cccttaaggc
     3721 gactcttccc attgctatcc accgggaact gctctagcac gacgttctcc gcggtctgtg
     3781 atcaacatcg catctccata actgtaaaac cggtagcgtg tctgcacggc ctcttcatac
     3841 gcgcggcgca gaaaccccgt acctgtgaac gcggaaacca acatcaggag ggtcgtctga
     3901 ggcagatgga aattcgtcaa gagcgcatct accaccttga actcaaatcc tggtctcacg
     3961 aaaaggctgg tttctcctcg atacggctgc agcgtcccgc gcaaagccgc tgtttcaatg
     4021 gcacgtacca ccgtagtacc gacggcgacg acctttcgtc caaccgcttt cacccgctcg
     4081 attgcacgca cggtggccgg tccgacttca acccattccg cctccatgcg atgctcttcg
     4141 atgcggactg cggtgactgg cttgaaggtc cccacaccga catgcagcgt gatcgtcgcg
     4201 agttcgatgt gtcgatccgt caaccggctc aggagagccg gagtgaaatg caggctggcg
     4261 gtaggcgccg caatggcccc ttcgtcccgc gcgaacacgg tttggtacca atcccgatcc
     4321 tgatcggttg gcggcctctt gatgtaggga ggcaatggca tgcggccatg catccgaaac
     4381 cactcgggga ttggcagggt tccgtcgatc cggacgaccg ttcgaccacc ctctctcgaa
     4441 agaacggtga catcggattc tccgtcgatt tcgatggttt gaccggcccg ccaggttccc
     4501 ttcatcagga cttcccactc acgatcggcc actggcttca caaaaagcat ctcgaccggc
     4561 cgtcctgagg ctcgtttcct ccccgtcaca cgtgccggtc tgactcgcgt atcattcacc
     4621 acgaggagat ccccgggatc gagcagatcg gcgagatcgg caatccggcg atgcatgatc
     4681 actcgattga ttggattcag aacgaggagc ttggcctgtt cacgcgggag cgtcggttcg
     4741 gtcgcgacaa gcgagggatc gaacgggaag tcaaattcag acagctgcat cgatctcttt
     4801 ggccgttcgc gcgagctctg tcttacgacg tcggagggat cggtggcttg gtgagggaca
     4861 tttcctgcag ttcagtgccg gggtaatagt aggacaaaat tgtggaaaac ggatacccga
     4921 gttcggccaa ttccttcgcg ccccactgac acatgccgac ggcatggccg gccccatagc
     4981 ccgatagcac gacctcccgg ccaacggaat caatcgtaaa ctgtgtgctt gggacgatcg
     5041 aatagccgac tgctttgcgg agatcctccc cgcgcaaaac gagctctccc tccgaatgaa
     5101 ggattcgcag cttcgcaaca cggccgccac gactgaatgc cagcggctcg atcgatgtga
     5161 tcgtcccgac cgcataccct tgctggcgca gattctgctc taatttatcc atccggaacg
     5221 acgccttcca ttgatagaag ggcgagtcca gatcgaacgg acattccacg cctttgagat
     5281 agggatagtc cttcgaccag acgttcaccg catcttcggt cagtcccgcg gcggtggagg
     5341 aaaaggcggc gtaaataggg gcttgttgat aggtcaacac gagaccccgg gtctcgtcaa
     5401 ccgcacgcaa cactgccgta tcgacgccat ccttcccttt gtagacttgg tcctgaaccg
     5461 ttgccacgac gtcatagtct cgtgaggcgc tcaacatctg ttgatacagt gcatacgtac
     5521 gtgcagccac ggcctgggcc ttcagcatct ccgggtgcca ggaagaattg ac
//
LOCUS       DASTQY010000034         7924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_380457, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000034 DASTQY010000000
VERSION     DASTQY010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380457"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1150
                     /locus_tag="VFR82_01130"
     CDS             281..1150
                     /locus_tag="VFR82_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEU4500229.1"
                     /translation="MRWQGQRQSENVEDRRGFGPARIGGVGGLGIGGIVLVLAVSYFT
                     GMNPLTLIDLMSGIQSTTTEPLIESVPTGPLKDEIGQFASVVLADTEDTWQQLLPKAG
                     GHYENPRLVLFTGAVHSACGATSSAVGPFYCPQDRKLYLDVSFFKEMSQRLGAPGDFA
                     QAYVIAHEVGHHVQNLLGIAEKVHQLQRRAGTAEGNALSVRLELQADCFAGVWGYHAN
                     RERKLIESGDFEEGLAAAAAIGDDRLQHMSTGYVQPESWTHGSSQQRKTWLRKGLDTG
                     NPEACDTFSSHQL"
     gene            1166..1615
                     /locus_tag="VFR82_01135"
     CDS             1166..1615
                     /locus_tag="VFR82_01135"
                     /inference="COORDINATES: protein motif:HMM:NF016798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUN14 domain-containing protein"
                     /protein_id="HEU4500230.1"
                     /translation="MIEEDACPTASSPAPSGWEAVLADPPWAGRSFLAVAVATVGGLA
                     SWIANYTSPTVAQLGGSYLGGYLIGWAFRRFLKMAAMMVAVGLALIGVMKTTGWVDVD
                     WNAVEAQVSQTVQWLHGEAQGLKNLLAGFLPSAGAAGAGSFFGFRKK"
     gene            complement(1639..1938)
                     /locus_tag="VFR82_01140"
     CDS             complement(1639..1938)
                     /locus_tag="VFR82_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500231.1"
                     /translation="MRGPVTTVCISTLFVAVLSYGMVSGADSEGGREASFSEKVGEGA
                     KKIGQAIAEGIKKTAKKIEDKHVPEKVERKLKKAADKTAEGFGKAEKKIKQKLAD"
     gene            2014..3033
                     /locus_tag="VFR82_01145"
     CDS             2014..3033
                     /locus_tag="VFR82_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU4500232.1"
                     /translation="MTGRCLCIATAVSTCLLAIFMGIPPCVSAETGAQPEVIERAKQA
                     AVGILEDTQDPRAPDKPGKIKIRGTGFHLHDGYVVTARHAVEKNSPSGPLVPKVIHVL
                     TTDLHEVVAQIVGESAYLDVVVYRLNQQDRPVLPVGAPFAVETPRVGSEVFTVGYPMG
                     WGPTMAFGRIGNANTFLQTVETRLLQADLAACSGNSGGALFNAGGEVVGIMHAVIQTD
                     KDESHVYCSQMAFAIPGTLAARIVTAAIEGKPLAFSKLGIHMTAVKDGTKWRIAVKDV
                     AEPAKSAGIQKHDILLAIEDTEILDAAHLKNYLIERTAPGQRVAVKVRRAEADLTFTV
                     TLVGG"
     gene            complement(3101..3907)
                     /locus_tag="VFR82_01150"
     CDS             complement(3101..3907)
                     /locus_tag="VFR82_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4500233.1"
                     /translation="MTAPSSYDGRILDIKHATIYRGDTCVFKDLSFVLEEDQHTAVLG
                     PNGAGKSTILKLLAGEVHAVPNDATSIRLFGDEQWNVWDVRRRLGMVSHDLQHQYMEQ
                     VTGLKVVLSGYYASIGTYGHQEFSYAHLARAHSVMEDLGIGSLTDRRYAAMSTGEQRR
                     CLLARALVHGPSVLVLDEPTSGLDLTATFHYLDLVRGYMRKGKTILLVTHHIHEIPPE
                     IRRVVLLKKGKVLQDGEKKAVLSEKHLSDLFDCPVALACANGWYQALPGS"
     gene            complement(3997..5160)
                     /gene="aroC"
                     /locus_tag="VFR82_01155"
     CDS             complement(3997..5160)
                     /gene="aroC"
                     /locus_tag="VFR82_01155"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU4500234.1"
                     /translation="MASNTFGHIFTVTSFGESHGPAIGCVVDGCPPGLALSAQDIQQD
                     LDRRKPGTSRHVTQRQEADTVEILSGVFEGKTTGTPVALLIRNEDARSKDYGSLIDTF
                     RPGHADYTYWQKYGIRDHRGGGRASARETAVRVAAAAIARKWLRETYGVLIHGYLSQL
                     GPHQVPFKTWETVSGNPFFAANAEMVGTLEAFMDELRKSGDSVGARITTVAEHVPVGW
                     GAPVYAKLDAELAAAMMSINAVKAVEIGSGFASVVQRGSEHGDELTPEGFVTNHAGGI
                     LGGISTGQDIVVTIGIKPTSSIRVPRRSIDKQGNPVTVETNGRHDPCVGIRATPIAEA
                     MMALVLMDHALLHRAQNAEVKTSTPKISGSVSVSATGGRVKPIAKVNPEPHEA"
     gene            complement(5180..6943)
                     /gene="aspS"
                     /locus_tag="VFR82_01160"
     CDS             complement(5180..6943)
                     /gene="aspS"
                     /locus_tag="VFR82_01160"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU4500235.1"
                     /translation="MKSRTHRCGELNRSHVGQTVVLNGWVQRRRDHGMVLFIDLRDRS
                     GITQVVFNAERNAAVHKAAHTLRSECVVSVTGTVAARPDESRNPNLSTGEIEVFADAV
                     EILNEAKTPPFVIEDDAEVTEAIRLKYRYLDLRRPRMQRLLSLRHGIMQATRGFLNAE
                     GFLEVETPILTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQVLMVSGVDRYYQIA
                     RCFRDEDLRNDRQPEFTQIDLELSFVDRGDVMSLTERMIATIFLDTGGVQLPTPFPRM
                     SYAEAMGKYGTDKPDLRFDMPLYDVTTFAGTSEFKVFREAAGKGKIVKALIVKGGSSI
                     ARSRIDALGETARSFGAKGLAWLKVASDGQLDSVIAKFLDAKAFAAALPEAKPGDLVL
                     FGADTPAVVHDVLGRIRLLLAEELNLIDKTAWKPLWVTDFPLLDYSQEEKRYVFMHNP
                     FAAPLDEDLALLDSDPLKVRAKAYDMVLNGNEIGGGSIRNHRSDVQLRILDLLGIGKE
                     QAQAKFGFLLDALDYGAPPHGGIAFGLDRLIMLLGGAESIRDVIAFPKTQRAQCPLTD
                     APSPVGADQLKELRIKLDLLE"
     gene            complement(6945..7805)
                     /locus_tag="VFR82_01165"
     CDS             complement(6945..7805)
                     /locus_tag="VFR82_01165"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU4500236.1"
                     /translation="MEIRLQKLIAGTGLASRRKAEGLIAAGRVMVNGKIVTELGTRVN
                     PERDHVKVDGKHLSAAQPFVYLMLNKPRNVVSTLDDPGGRTTVKDYLRGVSVRVFPVG
                     RLDFDSEGLMLLTNNGDLTQALLHPRYHVPKTYLIKVKGVLTDEDIAQLEQGVRLEDG
                     MTSPAQVKKVRKVEANSWLEITIREGRKHQVKRMLESVGHPVIKLMRIRMGPLSLGHL
                     EPGEFRFLTDREANALRQLVDERVALAEGGAEPARPSRRQPRRSGWARSTKKKVPRGR
                     AAWSSMRKRV"
BASE COUNT         1578 a         2411 c         2251 g         1684 t
ORIGIN      
        1 cagcgagaag tgctctcagc ggtgggatat gccgatacgc atcccattgc aagcaatgac
       61 tcagagcagg ggcgctcctc caaccggcgc atcgagatcg tgcttcaccc gaaagatctc
      121 tcgcaaatcg cgggcggcct gccccctgct gccaactgac gtctccctcg cgatcgatcg
      181 agcgaggcct tcctagccga aggcctcgcc accatcgcac acctggactt ctcctccgag
      241 tcgcagtatc gtggacgaaa ctgcttgagc gaggagagcc atgcgctggc agggccaacg
      301 tcaaagcgag aacgtggaag accggcgcgg attcggcccg gcgagaatcg ggggagtcgg
      361 cggactcggt atcggcggca tcgtgctggt actggcagtc agttatttta ccggcatgaa
      421 cccgctgacg cttatcgacc tgatgagcgg aatccagagc accacgactg agccgctcat
      481 tgagtcggtg ccgacgggtc ccctcaagga cgagatcggt caattcgcgt ctgtggtgct
      541 cgccgacact gaagatacct ggcaacagct gcttcccaaa gcgggaggcc attacgaaaa
      601 tccacgcctc gtgttgttca cgggcgcggt acactccgcc tgcggcgcga catcctcggc
      661 agtgggaccg ttttattgcc cgcaggatcg aaaactctac ctcgatgtct ccttcttcaa
      721 ggaaatgtcc caacggcttg gcgcgcctgg ggattttgcc caggcgtatg tgatcgccca
      781 cgaggtcggc caccacgttc agaatttgct cgggattgcc gagaaagtcc accaacttca
      841 gcgtcgggcc gggacagccg aggggaacgc cctgtctgtt cgcctggaat tacaggccga
      901 ttgctttgcc ggtgtctggg gctaccatgc caaccgtgaa cggaagttga tcgagtcggg
      961 cgatttcgaa gagggactgg ccgcagcggc ggctatcgga gacgaccgct tgcagcacat
     1021 gtctactggg tatgtgcagc cggagagctg gacacacggc tcctcgcaac agcggaagac
     1081 gtggttgcgc aaagggctgg acaccggaaa tcccgaggcc tgcgatacat tttcgtcaca
     1141 ccagctgtag atccaggaga aacacatgat cgaagaagac gcctgtccca ccgcttcctc
     1201 ccctgcacca tcgggctggg aggcggtcct ggctgatccc ccgtgggcag gaagatcgtt
     1261 cctcgccgtt gcagtggcca cggttggcgg gttggcgtct tggattgcga actatacgtc
     1321 tccgaccgtc gctcaactcg gaggaagcta tctcggcggt tatctcatcg gctgggcgtt
     1381 tcgccgtttc ttgaaaatgg cggcgatgat ggtggccgtc ggtctcgccc tcatcggtgt
     1441 gatgaaaacg accggctggg tggatgtgga ttggaacgca gttgaggctc aggtttccca
     1501 gaccgtccaa tggcttcatg gcgaagccca aggattgaaa aatcttctgg caggctttct
     1561 tccatcagcc ggtgcagcag gcgccggatc gtttttcggc ttccggaaga aataacccga
     1621 gcagaggccg ccggagggct aatcggcgag cttttgtttg attttcttct ccgccttgcc
     1681 gaagccttcg gccgttttgt ccgcggcctt cttcaatttc cgttccacct tttccggcac
     1741 atgcttatct tcgatcttct ttgcggtctt cttgatacct tccgcgatgg cctggccgat
     1801 cttcttggcc ccttcaccaa ccttttcgga aaatgacgcc tctctcccgc cttccgagtc
     1861 ggccccggac accatcccat aactcaggac ggcaacaaac agtgtcgaga tgcataccgt
     1921 ggtaactggc cctctcatgg ttccctctgt cattgatgtt ctcagctcat tatactgtgc
     1981 ggcatcggct tcatatagtg aaaggatgtt tttatgacgg gtcgctgtct ttgcattgcc
     2041 acggcagttt cgacttgcct gctcgcaatc ttcatgggta ttccgccatg cgtctccgca
     2101 gagacagggg ctcagccaga ggtcatcgaa cgcgcgaagc aggccgccgt cggcatcctc
     2161 gaagacacgc aagatccacg ggctcccgac aagccgggca agatcaagat ccgaggtacc
     2221 ggattccacc tgcacgacgg ctatgtcgtc acagccaggc acgctgtcga gaaaaacagc
     2281 ccgtccggac cactggtgcc gaaggtcatt catgttctga cgaccgatct ccacgaagtc
     2341 gtagcccaga tcgttggaga gagcgcctat ttggacgtcg tagtttaccg tctcaatcaa
     2401 caggaccgtc ccgtgctgcc agtcggggct ccctttgccg tggaaacgcc cagggtcggc
     2461 agcgaggtct ttacggttgg gtatccgatg ggatggggtc cgacaatggc gttcggccgt
     2521 atcggcaatg ccaacacctt cctgcaaacg gttgagacgc ggctgctgca agccgatctt
     2581 gcggcatgca gcggaaattc cggaggcgcg ctctttaatg ccgggggcga agtcgttggc
     2641 atcatgcatg ccgtgatcca aacggacaaa gatgaaagtc acgtgtattg cagtcagatg
     2701 gcctttgcca ttcccggtac tttggccgca cggatcgtaa cggcggcgat cgaaggaaaa
     2761 ccgttggctt tctcaaaact gggcattcac atgactgcag tgaaagacgg cacgaaatgg
     2821 cgcatcgccg tgaaggatgt ggcggagcca gcgaaaagtg caggcattca gaagcatgac
     2881 atcctgctgg ctatagaaga cacggagatt ctggacgccg cacatttgaa gaactacctg
     2941 atcgaacgaa cggctcccgg ccagcgggtc gcagtcaaag ttcgtcgggc cgaggcagat
     3001 ctgacgttta ccgtgacgct ggtcggcgga taggggatgg ctgaaggtag atggtgccgc
     3061 gtttcggcgc gaaccgggtg ggcggtgaag aatggctgtt tcagcttccg ggtaatgcct
     3121 gataccagcc attggcacaa gccagcgcga ccgggcaatc gaacaggtcg ctcagatgtt
     3181 tttctgacaa caccgccttt ttctctccgt cttgaagaac cttccctttc ttcaacagga
     3241 cgactcttct gatctccggc ggtatttcgt ggatgtgatg cgtcacgagc agaatggttt
     3301 tccctttccg catataccca cgcacaagat ccaaatagtg aaaggttgcc gtcagatcga
     3361 ggccgcttgt cggctcatcg agcacaagaa ccgatggccc atgaacgaga gcccgggcga
     3421 gcagacaacg tcgctgttca ccagttgaca tggcggcata ccgccggtcg gtcagggagc
     3481 cgatgcccag atcttccatc accgaatggg cccgggcaag atgcgcatag gagaactcct
     3541 gatggccata cgtcccgatg ctcgcgtaat aacctgacaa cacgactttc aaaccggtca
     3601 cctgctccat gtactgatgc tggagatcgt gcgagaccat ccccagacgc ctccgtacgt
     3661 cccagacgtt ccactgctca tcgccgaaca gccggatgga tgtcgcatca tttggcaccg
     3721 cgtgcacttc gcccgccagc agtttcagaa tcgtagactt ccctgctccg tttggcccaa
     3781 gcaccgccgt atgctggtct tcctccagca cgaaagagag gtctttaaag acgcaggtgt
     3841 cgccgcggta gatggtcgcg tgtttgatgt cgaggatgcg gccgtcgtag gaactcgggg
     3901 cggtcatgcc gtgagggtac accgccgaag gctcaaacag tgaaaagatc ttgtggactg
     3961 gatggtgtgg aacaacgcat tcgacatctc aggacgtcag gcttcgtggg gttctgggtt
     4021 caccttcgcg attggtttca ccctcccgcc ggtggctgaa acggagaccg atccgctgat
     4081 ttttggagtc gaggtcttga cctctgcatt ttgcgcccga tgaagcagcg cgtggtccat
     4141 gagcaccagt gccatcatcg cttccgcgat gggagtggcc cggatgccaa cacagggatc
     4201 atgacgtcca ttcgtctcaa ccgtcacggg attgccctgc ttgtcgatag agcggcgggg
     4261 gactcgtatg ctcgacgttg gcttgatgcc gatcgtgacg acgatatcct gaccagtcga
     4321 aatacctccc aggatgccgc cagcgtgatt cgtgacgaaa ccctcaggcg tcagttcgtc
     4381 accatgctct gaacctcgtt gcacaacgga ggcgaaccct gagccgattt ccaccgcctt
     4441 caccgcgttg atgctcatca tcgctgctgc cagttcggca tccagcttgg catacacggg
     4501 cgccccccag ccgaccggaa catgctcggc caccgtcgtt atcctggcgc cgaccgaatc
     4561 gccggatttc ctcagctcat ccataaacgc ttcgagtgtc ccgaccatct cggcattggc
     4621 ggcgaaaaat ggattcccgc tgaccgtctc ccaggtttta aacggaacct ggtgtgggcc
     4681 cagctggctc agatagccgt gaatgaggac gccgtacgtc tcccggagcc acttcctggc
     4741 aatggcagcc gcggcaacac gcacggcggt ctcgcgcgca gacgcgcggc caccgccgcg
     4801 gtgatcccgg attccgtatt tctgccaata cgtataatcg gcatgaccag gacgaaacgt
     4861 atcgatgagg ctgccatagt ctttgctccg cgcgtcttcg ttgcggatca gtaacgccac
     4921 gggtgttccc gtcgttttac cttcgaagac gccggaaaga atttcgaccg tatcggcttc
     4981 ctgccgctgc gtcacgtggc gcgacgtacc gggcttgcgg cggtccagat cctgttggat
     5041 gtcctgagcg gagagcgcaa gcccaggagg gcagccatca acgacacagc cgatcgcggg
     5101 cccatgactc tcgccaaagg aggtgaccgt gaagatatga ccaaaggtat tgctggccat
     5161 tgcgcgaatc ctctctggtt cactccagca gatcgagctt gatgcgcaac tccttcaact
     5221 gatcggcgcc gaccggtgac ggagcatccg tgagggggca ttgggcgcgt tgcgtttttg
     5281 gaaacgcaat gacgtcgcga atggactctg cgcccccaag cagcatgatc agccggtcga
     5341 ggccgaaggc gatgccgccg tgcggaggcg ctccgtaatc cagagcatcg agcaaaaagc
     5401 cgaacttggc ctgcgcctgc tccttgccga ttcccaggag atccaggatg cgcagctgta
     5461 catcactgcg atggttgcga atgctgcctc cgccaatttc gttgccgttg agcaccatgt
     5521 cgtaggcctt ggcacgcact ttgagcgggt ctgagtccaa caaagccagg tcctcgtcaa
     5581 ggggagcggc aaagggattg tgcatgaaga cgtagcgttt ctcttcctgc gaataatcga
     5641 gcaaaggaaa atcggtgacc cagaggggct tccacgctgt cttgtcgatc aggttgagct
     5701 cttctgctag cagcaaccgg atgcggccca acacgtcatg aacgacggcg ggagtgtccg
     5761 ccccgaacag caccaggtcg ccgggcttgg cttccggcaa ggcggccgca aatgctttgg
     5821 catctagaaa tttggcaatg accgaatcca attgaccatc gcttgccacc ttcagccacg
     5881 ccaggccctt ggccccgaag ctcctggcgg tttctcccaa ggcatcgatc cggctgcgtg
     5941 cgatgctgct cccccctttg acaatgagcg ccttgacgat ctttcccttt ccggccgctt
     6001 cgcgaaacac cttgaactcg ctcgtcccgg caaaggtcgt gacgtcgtac aacggcatgt
     6061 cgaaccggag gtctggtttg tccgttccat acttccccat cgcctctgca tacgacattc
     6121 gaggaaacgg cgtcggcagc tgcacgccgc cagtatcgag gaagatcgtg gcaatcattc
     6181 gctcggtgag actcatcacg tctccgcggt cgacaaatga cagctccagg tcgatttgtg
     6241 tgaactcggg ttgacggtcg tttctgaggt cttcatccct gaaacaacgg gcaatctggt
     6301 agtaccgatc cacgccgctc accatgagga cttgcttaaa aagctgcggt gactgcggaa
     6361 gggcaaaaaa ctgccccgcg ttgacccggc tgggcaccag gtagtcgcgc gcgccttccg
     6421 gcgtgctttt cgtcagaatc ggcgtttcaa cttcaagaaa cccctccgca ttcaagaaac
     6481 cccgcgtcgc ctgcatgatc ccgtgccgca ggctcaacaa ccgctgcatt ctcggccggc
     6541 gtaagtcgag atagcgatac ttcaagcgga tcgcttccgt gacttcggca tcgtcctcga
     6601 tgacaaatgg cggtgtcttg gcctcgttca aaatctcgac ggcatccgcg aagacttcaa
     6661 tttcaccggt cgacagattg gggttcctgg actcatccgg acgggctgcc accgtcccgg
     6721 tcaccgaaac gacgcactcg ctccgtaggg tatgggccgc tttgtgcacg gcggcattgc
     6781 gctccgcatt gaagacgact tgcgtgatac cgctccggtc cctcaggtcg ataaacagca
     6841 ccatcccgtg atcgcggcga cgctgcaccc acccgttcaa caccacggtc tggccgacat
     6901 gggacctgtt caactctccg caccggtgcg ttctgctctt cacatcagac tcgctttctc
     6961 atggaggacc atgcggctcg accccgtggc acttttttct tggttgaacg cgcccacccg
     7021 gaacgcctcg gctgcctcct ggacgggcga gccggttccg ctcctccttc agccagtgcc
     7081 acccgttcgt ctacgagttg gcgcagcgca ttcgcctcgc ggtcggttaa gaaccggaac
     7141 tcgccaggct ccaaatggcc gagggagagc ggccccattc tgatccgcat cagtttgatg
     7201 accggatggc cgactgattc gagcatccgc ttgacttggt gtttacgtcc ctcgcgaatg
     7261 gtgatttcca gccaggaatt cgcctcgacc tttcggacct ttttgacctg cgccgggctc
     7321 gtcatcccgt cctccagccg caccccctgt tccaattgag caatgtcctc atccgtcagc
     7381 acgcccttca ccttgatcag atacgtcttg ggcacatggt aacgtgggtg gagcagggcc
     7441 tgggtaagat ctccgttgtt ggtcaacagc atcagtcctt cgctgtcgaa atccaatcgg
     7501 cctactggaa acacgcgcac ggacacgcca cgcaagtaat ccttgacggt ggtgcgccct
     7561 cccggatcgt cgagcgtcga cacgacattc cgaggcttgt tgagcatcag atacacaaac
     7621 ggttgtgctg cgctgagatg cttgccgtcg accttgacgt gatcccgttc cggattcact
     7681 cgggtgccca gctccgtgac gatctttccg ttaaccatca ctcgaccagc cgcgatcaag
     7741 ccctcggctt ttcgccgaga cgcgaggccg gttccggcta tcaatttctg aagtctgatt
     7801 tccatgtccc ctcactgacg gtcgaccagg atggcccaac tgcacgcccc attgtttacc
     7861 gaccgatccc cgccgcgcga gcgcgactta tacccaacac cgcttacaat gatccgtccg
     7921 agct
//
LOCUS       DASTQY010000035         4690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_805104, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000035 DASTQY010000000
VERSION     DASTQY010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805104"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..2165
                     /gene="uvrB"
                     /locus_tag="VFR82_01170"
     CDS             159..2165
                     /gene="uvrB"
                     /locus_tag="VFR82_01170"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEU4500237.1"
                     /translation="MSPFQLEAPFKPCGDQDQAIDQLVAGIQDGRKHQALLGVTGSGK
                     TFTMANVIARVQKPTLVLVHNKTLAGQLYQEFKQFFPHNAVEYFISYYDYYQPEAYIP
                     QTDTYIAKDSSINDAIDQMRHAATTSLLQRNDVLIVSSVSCIYGLGSPEVYHGMVVYL
                     EEGMEIRREKILAKLVEIQYARNDVDFHRGTFRARGDVIEIFPASNEAVSVRIELFGD
                     VIDAIHEIDPLTGKSIGKLPKVAIYPNTHYLIAPDRYQRAITGIEDELDERVAYFKTH
                     NQLLEAQRLTQRTKFDLEMIRAMGYCHGIENYSRHLSGRAPGEPPPTLIDYFPKDFLL
                     IVDESHATIPQVGGMYEGDYSRKRTLVDYGFRLPSAVDNRPLKFPEFERCMNQVIYVS
                     ATPGPYELEHTGGVVVEQIIRPTGLLDPVIDVRPAKGQVDHLLGDVRAEIALGGRVLV
                     TTLTKRMAEDLCEYYHELGIKVRYLHADIKTFERAEIIRDLRLGRFDVLIGINLLREG
                     LDLPEVSLVAILDADKEGYLRSHRSLIQTAGRAARNVRGRVLMYGDVITASMQRAINE
                     TSRRRAIQAAFNEAHGIVPVSIQKEVLQLDYAEASQEPEQLTLAAEAAVDYTPEEGPV
                     EQQIRRLEVEMKAAAKDLEFERAATIRNRIRALKLRDLESKSGA"
     gene            complement(2235..2495)
                     /locus_tag="VFR82_01175"
     CDS             complement(2235..2495)
                     /locus_tag="VFR82_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HEU4500238.1"
                     /translation="MRKSPWLLLVFLLIGGLLGGILGEVLRVMAPQGTIQTVFATNFT
                     PGISPPLTIDLVLLKITLGFSFKISLLSILGMLLGIYLYKNV"
     gene            2709..4055
                     /gene="ffh"
                     /locus_tag="VFR82_01180"
     CDS             2709..4055
                     /gene="ffh"
                     /locus_tag="VFR82_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU4500239.1"
                     /translation="MLDQLSDKFEKILKKLRGTGVLTEENIAEALKEVRLALLEADVN
                     YKIVKDFVERVRQKAIGQEVLHSLTPGHHVVKVVWDELRAIMGQERAGLSLASKPPTV
                     VMMVGLQGSGKTTTCGKLAKLFKQQGKRVLLVAADPRRPAAGDQLASIGRDLEVDLHR
                     LDRPGTAQDDVVRICRTGVERGQEQGFDLVVLDTGGRLHVDDDLMNELVAVKSAVQPN
                     EVLLVADAMTGQDAVTMATQFDQRVGLTGIILTKVEGDARGGALLSIRAVTGKPVKFL
                     GVGEKLDALELFHPDRMASRILGMGDVLTLIEKAQETFTREQAEEAQQRLTSDTFTLE
                     DFRSQLGQVNKLGSLDQILGMLPGGQKLKDAMAGQVPERELKRVMAMIDSMTLRERRD
                     HTVINGSRKKRIARGSGTNVTEVNRLIKQFLSARKIAKAMTGTGGRRQLAQLLRSM"
     gene            4154..4438
                     /gene="rpsP"
                     /locus_tag="VFR82_01185"
     CDS             4154..4438
                     /gene="rpsP"
                     /locus_tag="VFR82_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU4500240.1"
                     /translation="MAVHLRLTRTGRHKRPIYRVVAADSRKPRDGRFLEILGIFDPLK
                     EAGLPELKQERVLSWLHQGAQPTVTVKTLLRRSGVWKQFETEKAAKKNKA"
     gene            4440..>4690
                     /locus_tag="VFR82_01190"
     CDS             4440..>4690
                     /locus_tag="VFR82_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500241.1"
                     /translation="MTALWPDMVTIGKVERPFGVHGAVKVRSLSDRPGRFEQLHEVRV
                     AGERGQTVDRRVRSLRKAGRVYIIEFEGITTPEEARVLQG"
BASE COUNT         1047 a         1300 c         1415 g          928 t
ORIGIN      
        1 ccgcgacctg catttcgttc agagaagccc cgacggttcg ggcgataatc ctcgcccgcg
       61 catcgccgta tcacgatcga ccaagcggtc ggtcgtgatg tgcttctcag gaagggttcc
      121 cagtccgcat ggtagagtaa ccacctgtag gacgcctcat gtcaccgttc caactcgaag
      181 ctccatttaa accctgcggg gatcaggatc aggccattga tcagctcgtg gccgggatcc
      241 aggatggtcg gaaacaccag gcgttgcttg gcgtgaccgg gtccggcaaa acgttcacga
      301 tggccaatgt catcgcacgc gtgcaaaaac cgacgctcgt gctggtgcat aacaagacgc
      361 tcgccggcca gctataccag gagttcaagc aattttttcc ccacaatgca gtcgagtatt
      421 tcatcagcta ttacgactat taccagccgg aagcctacat tccgcagacg gacacttata
      481 ttgccaagga ttcgtccatc aacgatgcga tcgatcagat gcggcacgcg gcgacgacct
      541 ccctcctgca gcgcaacgac gtgttgatcg tctcatcggt gtcgtgcatt tatggtcttg
      601 gttctccgga ggtgtatcac gggatggtcg tctacctcga ggaagggatg gagatcaggc
      661 gtgagaagat tctggcgaag ctcgtcgaaa ttcaatatgc gcggaacgac gtcgatttcc
      721 atcgcggtac cttccgagcc cgtggcgacg tcattgaaat cttcccggcc tcgaacgaag
      781 cggtgtccgt ccgcatcgaa ctattcggcg acgtcattga cgccattcat gaaatcgatc
      841 cgctgacagg caagtcgatc ggaaaattgc ccaaagtggc catttatccg aatacccact
      901 acctgatcgc tccggatcgc tatcaacggg cgatcacggg gattgaagac gagctcgatg
      961 aacgagtggc ctacttcaaa acacataatc agttgttgga agcccagcgc cttacgcagc
     1021 ggacgaaatt cgatttagaa atgatccgcg ccatgggcta ttgccatggg attgagaact
     1081 attcccgcca tctgagcggg cgggctccgg gggagccacc gcccaccctg atcgactact
     1141 tccctaaaga ctttcttctc atcgtcgacg aatcgcatgc caccattccg caagtcggcg
     1201 gcatgtacga aggcgactat tcgcgcaaac gcacgctggt cgactatggg tttcgtctgc
     1261 cttctgcggt cgacaaccgc ccactcaagt ttcctgagtt cgagcgctgc atgaaccagg
     1321 tcatctatgt gtccgccacg ccgggtccgt acgagttaga gcacaccggc ggggtcgtcg
     1381 tcgagcagat cattcggccg acgggcctcc tcgatccggt catcgacgtg cgacccgcga
     1441 aaggccaggt cgatcatctg ctgggcgacg tccgggccga aatcgcgctg ggagggcgcg
     1501 tactggtgac gacgttgacc aagcgcatgg ctgaagacct gtgcgagtac tatcacgagc
     1561 tcggcatcaa agtgcgctac ctccatgcgg acattaagac gttcgagcga gccgagatca
     1621 ttcgtgacct ccggctgggc cgtttcgatg tcctcatcgg gattaacctg ctacgggaag
     1681 gactagacct gccggaagtc agtctggtgg ccatattgga tgccgacaaa gaaggctatc
     1741 tgcggtcgca tcgttccttg atccaaacgg caggacgtgc cgctcgaaac gtgcgcggcc
     1801 gggtgctcat gtatggggat gtgatcaccg cttcgatgca gcgggcgatc aatgaaacct
     1861 cccgccgccg agcgatccag gctgctttca acgaggcgca cggtattgtt ccggtgagta
     1921 ttcagaaaga agtgctgcag ctggactacg cggaggcttc tcaggagcct gaacagttga
     1981 cgttggctgc cgaagcggcc gtggactaca cgccagagga ggggccagtc gaacaacaga
     2041 tccggcggct cgaggtagag atgaaagcag ccgcaaagga tttggagttc gaacgcgcgg
     2101 ccacaatacg caaccgcatc cgggcactca agctgaggga cctggagtca aaatccgggg
     2161 cctagcgccc acccaccccc ttgtccgcaa taggcggtgc gtcgagtacc acgtctcgca
     2221 ccgtttgccc gccgctagac gttcttgtac aggtagatgc cgaggagcat gcccagaatg
     2281 ctgaggagac tgatcttgaa actaaacccc agtgtgatct tgagaagaac cagatcaatc
     2341 gtcagcggag ggctgattcc gggggtgaag tttgtggcga agacggtctg gattgtcccc
     2401 tgcggggcca tcacccgcag gacctctcca aggataccgc cgagcaagcc gccgatgagg
     2461 aggaatacca gcagcagcca gggcgatttt ctcacgtcaa cggcctccta aactattgac
     2521 tgcattcgcg gaattctcga agtcgaccat agcgaaaggt ctccaaggtg tcaaccggaa
     2581 tgccgttctc tgcgccttga cttctccgga ggcctccatt agactgcccg gtgctctttt
     2641 gctagatttt tcgaggcgat cgaagatcta cgggtacggc ttcgattgcc ttttttcttt
     2701 ggtgtgcgat gctggatcaa ctgagcgaca agttcgaaaa aatcctgaaa aaacttcgcg
     2761 gaacgggtgt cctgaccgaa gagaacattg cggaagccct caaagaagtg cggctcgccc
     2821 tcctggaagc ggacgtcaat tacaaaatcg tcaaagactt cgtcgagcgg gtgcgtcaaa
     2881 aggcgatcgg acaggaagtc ctccatagtc tgacgcctgg tcaccacgtc gtgaaagtcg
     2941 tatgggacga actccgggcc atcatggggc aggaacgggc tggcctctct ctggcctcaa
     3001 agccgcctac agtggtgatg atggtcgggc ttcagggatc aggcaagacc acgacatgcg
     3061 gcaaactcgc caaactcttt aagcaacaag gcaaacgggt gttgctcgtg gcggccgacc
     3121 ctcgccgtcc cgcggctgga gaccagctgg ccagtatcgg acgggatttg gaggtcgacc
     3181 tccatcgcct tgaccgccca gggacggcgc aggacgacgt tgtccgcatt tgccgcaccg
     3241 gcgtcgagcg tggacaagaa cagggatttg acctggtggt gctcgatacc ggcggccgcc
     3301 tgcacgtgga tgatgacctc atgaatgagc tcgttgccgt gaagtctgcg gtgcagccca
     3361 atgaagtcct gttggttgca gatgctatga ccgggcagga cgccgtcacg atggccaccc
     3421 agttcgatca gcgcgtgggt ctgaccggga tcattctcac gaaagtggaa ggcgatgcgc
     3481 gtggcggtgc gctgctttcg attagggcgg tgacgggaaa gccggtcaaa tttctcgggg
     3541 tcggcgaaaa gctggatgcg ctggaactgt tccaccccga ccggatggcg tcgcgcattt
     3601 tgggaatggg cgatgtcctg actctcatcg aaaaggcgca agagaccttc actcgcgaac
     3661 aagccgaaga agcgcagcag cggctgacca gcgacacctt tacgcttgag gatttccgct
     3721 cacaactggg tcaggtgaac aaattgggct cgctggatca gatcctcggc atgctgccag
     3781 gtggtcagaa attgaaagac gcgatggcgg gacaggtgcc cgagcgggag ttgaaacggg
     3841 tcatggccat gatcgattcc atgacgctgc gcgagcggcg tgaccatacc gtcatcaacg
     3901 gaagccgcaa aaagcggatt gcccgcggaa gcggaaccaa cgtgacggaa gtgaatcggc
     3961 tgatcaagca gtttctctcc gcgagaaaaa ttgcgaaggc gatgacggga accggaggac
     4021 gtcggcagtt ggcgcagttg ctccgctcca tgtgatgtgg cacggctatg ggcgatgagc
     4081 ctccggcttc gtgcaagtcg aatacaccag tcccaaccgt gagttgggat ttcactgttg
     4141 aaggaggaat ccagtggccg ttcatttgag attgactaga accggacgac ataaacgacc
     4201 gatctatcgc gtcgtggcgg cggattcccg caagcctcgc gacggtcggt tcttggaaat
     4261 tttgggcatc ttcgatccgt tgaaagaagc gggacttccc gagttgaagc aggagcgggt
     4321 gctttcctgg ttgcatcagg gggctcagcc cacggtgacc gtcaaaacac tgcttcgacg
     4381 ctcaggagtc tggaagcagt tcgaaacgga aaaagccgcc aaaaagaaca aggcctagtg
     4441 tgactgccct ctggcctgac atggtaacga tcgggaaagt cgaacgaccc ttcggtgtcc
     4501 acggagcggt caaggtgcgg tcgctgagcg accggccggg ccggttcgag cagttacacg
     4561 aggtccgtgt ggccggtgaa cgagggcaga cagtcgatcg gcgggttcga tcgctgcgca
     4621 aagccggtcg ggtctacatc atcgaatttg agggcatcac aacaccggaa gaagcacggg
     4681 tgcttcaggg
//
LOCUS       DASTQY010000036         4432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1184585, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000036 DASTQY010000000
VERSION     DASTQY010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184585"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFR82_01195"
     CDS             complement(<1..377)
                     /locus_tag="VFR82_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500242.1"
                     /translation="MAGLLAVMLQLAGEGRLCDVAAADWGKPLGALYDGVEVRPRPVT
                     PAPTELGPDERATMAVFERATKSVVFISNTAIQRDFWSFDVLEVPQGSGSGFVWNKQG
                     HIVTNFHVIYGANTIKVTLADRS"
     gene            complement(395..817)
                     /locus_tag="VFR82_01200"
     CDS             complement(395..817)
                     /locus_tag="VFR82_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEU4500243.1"
                     /translation="MAVQTLPLRIDMRGGTQIENVTKFVQEALAHAKMSAGIVTVFVQ
                     HTTASVMVIEDEPGIRSDTKTFWDRIAPADQSWQHNEQNPGEDNGHSHLRGQLQGPSV
                     TIPFSNGSLLLGTWQQLVVVDFDTRARTRDLIIQILGE"
     gene            complement(820..1680)
                     /locus_tag="VFR82_01205"
     CDS             complement(820..1680)
                     /locus_tag="VFR82_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500244.1"
                     /translation="MDTQRAPLLVSRSLVSRLMRLYDVSHPLHKRRVIRGYDRAHAVR
                     TARMCAAVASALGHGTERVRQYQIACLLHDLGRAGLDRRLFGKIWSWAKAHGIPTRPR
                     EWRAIHPETPYGRETEAFVARYREALEADGIPVTAWAKEQIEMRLGYARRLAKRLRTV
                     RSSITRMGVRFEPWMQLVMLYYYYPEKLSRARPWVKQLAEVLVACEQFEAYSNQRRGR
                     DYYVRSKESIAEAFAYLDKLEQERILSVEVMKVMKRLAAAGEFNGVIAAARGRRLTPQ
                     ERRFLRRMEG"
     gene            1827..2453
                     /locus_tag="VFR82_01210"
     CDS             1827..2453
                     /locus_tag="VFR82_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500245.1"
                     /translation="MRMYHLTTTLLIGTLLIAGTLNIRPAAASPTEQLADLTGKTSVS
                     VTFTGRDNFNSEYRYDLTVRNRSSDPFIADSLIVVLEKITNLAGEDREPLKSEPLLNR
                     FEIVGQDGETEDGKPFFRVPVGASPDLAPLTDSLPLSIRIRNRDYVSVFTPSFRVYGK
                     KRPPAEPKPSVSQAQPAPSPQDRQTDRLIQLLLKKGIITEEERKTIQP"
     gene            2450..2866
                     /locus_tag="VFR82_01215"
     CDS             2450..2866
                     /locus_tag="VFR82_01215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU4500246.1"
                     /translation="MSHPPCDACTGIWPAADHFIADLGLSLAYLHDDQFFPGWTVLLF
                     KRHATELFHLAPTERIQLMEEVSGLAKVLAETFQARKMNYELLGNQLPHIHWYLIPRL
                     AHDPAPIEPVWRVPHDRIALSAEQVGTTISLIKKHL"
     gene            2958..4181
                     /locus_tag="VFR82_01220"
     CDS             2958..4181
                     /locus_tag="VFR82_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4500247.1"
                     /translation="MRRRLSAVTIFSACVAASSFAQGPDDPTPLAPHESPPPPPEAVG
                     DESPHTFVDPGHAAKRLTELRGLVRLSPRHAGERIALAEALYDTGDLDAAIGECRLAV
                     KLDPNSAKAHSQLGVMLMAKQDWRAAASVLAEALRLDPALTDAHYSLGSVYYSLGDRK
                     AAIQSYRQALALQPHFPDARYRLALLLMLSSQTQEAAQLMEEAAIGGVSQAQYFLGNA
                     YKSGQGVEKDLAKTIFWWMKGVEYGYQPAADSLSKLRRQALSADQPEKKRKEALDAFQ
                     RYRDKLWEEFHDYGSMEDGRTLGTTLLADGRTDVAVPVLLKEGYALSEVAQAALARLY
                     ESGSGTALEPYDKKILACFETTAAEGFVPAKRLLARVYGRGIGVPQDLPKAKGILKGL
                     PKNEMNAFLTEIGGR"
     gene            4185..>4432
                     /locus_tag="VFR82_01225"
     CDS             4185..>4432
                     /locus_tag="VFR82_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500248.1"
                     /translation="MQIGWSRFWRLFITTPLLQAFVIGLLASVLATSLWSLAPVTSTT
                     LDWTLYDTWVRHRVPIATSPILAIVVSDPQSEARLGTV"
BASE COUNT          945 a         1377 c         1213 g          897 t
ORIGIN      
        1 tcgctgcggt ctgccaaggt gactttgatc gtattcgctc cataaatcac atgaaaattt
       61 gtgacaatat gcccctgctt attccacacg aagcctgagc cggatccttg ggggacctcc
      121 agaacgtcga aggaccaaaa atcccgctgg atggcggtat tcgagataaa cacgaccgac
      181 ttggtcgctc gctcgaagac cgccatcgta gcccgttcat cagggcccag ctctgtgggg
      241 gccggggtaa ccggccgtgg ccgcacctcg acaccatcat agagcgctcc gagcggtttc
      301 ccccaatcgg cagccgcaac atcacacaaa cgcccttcgc cagccagttg gagcatcacg
      361 gccagcagtc ccgccatgac agaaaccatc aaggctactc tccgaggatt tgaatgatga
      421 gatcccgagt gcgcgcgcgg gtgtcgaaat ctaccacgac gagctgttgc catgtcccca
      481 atagcagcga cccgttcgaa aagggaatgg tgacggaagg accttgcaac tggcctctca
      541 gatgactatg gccgttgtct tctcccggat tttgctcgtt atgctgccag gactgatcag
      601 ccggggcgat ccggtcccaa aacgttttcg tgtccgatct gatccctggc tcgtcctcga
      661 tgaccatgac cgacgcggtg gtatgctgaa cgaacacggt cacgatgccg gcagacatct
      721 tggcatgagc cagcgcttcc tggacgaact tcgtcacatt ctcgatctgg gttccgccgc
      781 gcatgtcgat ccggagaggc agcgtctgga ccgccatact cacccctcca ttcgacgaag
      841 aaatcgtcgc tcttggggcg tcaaccggcg gcctcgtgct gccgcaatca ccccgttgaa
      901 ctccccggcg gcggccagcc gtttcatcac cttcatcacc tctacgctga gaatccgctc
      961 ctgctccaat ttatccaggt acgcgaaggc ctccgcgatg gattctttgc tacgcacata
     1021 atagtcgcgg ccgcgacgct ggttgctgta cgcttcgaac tgctcgcagg cgaccagcac
     1081 ctccgccagc tgtttcaccc agggccgggc ccggctcagc ttttccggat agtagtaata
     1141 cagcatcacg agctgcatcc agggctcaaa tcgtacaccc attcgcgtga ttgacgatcg
     1201 caccgtccgt aacctttttg ccaaacggcg ggcgtagccc agccgcattt ctatctgttc
     1261 ctttgcccag gcggtgacgg gaatgccgtc ggcttcgagc gcttctctat agcgggcgac
     1321 gaacgcttcg gtctcgcggc catagggcgt ttcaggatgg atggcccgcc actcacgagg
     1381 cctcgtcgga atcccgtgag ccttcgccca ggaccagatc tttccgaaca gccggcgatc
     1441 caggccggcg cgtcccaagt cgtgcaaaag acaggcgatt tgatactgtc gcacacgctc
     1501 ggttccatgt cccaatgcag aggccacggc agcgcacatg cgcgccgtgc ggacggcatg
     1561 tgcccgatca taacctcgaa tgactcgtcg cttgtgaaga ggatgagaca cgtcgtacag
     1621 gcgcatgagc cgggaaacga gggagcggga aaccaggagg ggggcgcgtt gggtgtccat
     1681 cgactcttcg actcgctttt ttgccgcgcg cagaatatct gtcgaagaaa cagagtgtca
     1741 aggcaagatc gcgagaacac tcgatcaatg ttgagatgtt tgctatgctt cgcgtgcaat
     1801 cgtgatcttg aatcgggcga ccactcatgc ggatgtatca cctgaccacc actctgctga
     1861 tcggaacgct gctcatcgcc gggacgctca acatacgtcc agcggccgcc tcgcccaccg
     1921 aacaactggc tgatctcacc ggcaagacgt ccgtcagcgt gacctttacg ggtcgcgaca
     1981 acttcaacag cgagtaccga tacgatttga ccgtccgtaa ccggtccagc gatcccttca
     2041 tcgctgattc actcatcgtc gtgctggaga aaatcaccaa tctcgccggc gaagaccggg
     2101 agccgctgaa aagtgaaccg ctcctcaatc gctttgaaat tgtggggcag gacggagaaa
     2161 cggaagacgg caagccattt ttccgcgtac cggttggcgc cagccctgat ctggctccgc
     2221 tgaccgacag ccttccgctt tccattcgca ttcgtaacag ggattacgtg tccgtcttca
     2281 ccccgtcatt ccgcgtgtac ggaaaaaaac gaccacccgc ggaacccaag ccatcggtca
     2341 gtcaggcaca gccagcccct tcgccacagg atcggcagac tgacagactg atccaactgc
     2401 tcctcaaaaa aggcatcatc accgaggaag agcggaaaac gatccagcca tgagccatcc
     2461 gccgtgtgac gcctgtacag gaatatggcc tgctgccgat cacttcattg ccgacctggg
     2521 tctgtccttg gcctatctgc acgacgatca gttctttccc ggctggaccg tgctcctctt
     2581 caagcgtcat gcgacggaac tattccatct tgcgccgacc gagcgcatcc aactgatgga
     2641 agaagtcagc ggcttggcca aagtgctcgc cgaaacgttt caggcaagaa aaatgaatta
     2701 cgagttgctg ggcaatcagc ttcctcacat tcattggtac ctcattccgc gccttgcgca
     2761 cgatccggct cctatcgaac cggtctggcg cgtcccacat gaccgcatcg ccctctccgc
     2821 ggaacaggta ggcacgacca tttcgctgat caagaagcat ctgtgaccga ctccacacat
     2881 cgtgcgcctt atcgacgaac cactctccgc tcggtggtat aatgtcgccc attcgatgaa
     2941 cggaggagcc taggtcgatg cgccgtcgat tatcggccgt gaccatcttc agcgcatgcg
     3001 tcgctgcttc gtcatttgct caaggccctg acgatccgac tccgcttgct cctcacgaaa
     3061 gtccaccccc gcctccggaa gccgtcggcg atgaatctcc tcacacgttc gtcgacccag
     3121 gtcatgccgc aaaacgtctg accgaattgc gaggtctagt ccgactgtca ccgaggcatg
     3181 caggcgagcg catcgccctc gcggaagcgc tctacgatac cggcgatctt gacgcggcga
     3241 tcggagaatg ccgccttgcc gtcaaattag atcccaacag tgcgaaggcg cactcacaat
     3301 tgggcgtcat gcttatggcc aagcaagact ggcgggcagc cgcttcggtt ctcgccgagg
     3361 ctctccggct cgatcccgca ttgacggatg cgcattacag tcttggaagc gtgtactact
     3421 cattgggcga ccggaaggca gcgatccaat cctatcgaca ggcgctcgcc ttgcaaccgc
     3481 atttcccgga tgcacgctac cgcctagcgc tgctcctcat gctgtcgagc cagacacaag
     3541 aagcagcaca actgatggag gaggcggcga tcggaggcgt ttcacaagcc cagtactttc
     3601 tgggcaacgc gtacaagagc ggtcaagggg tggagaaaga tctggcgaaa accattttct
     3661 ggtggatgaa gggtgtcgaa tacggctacc aaccggctgc cgatagcctc tccaagctcc
     3721 gacggcaagc gctctcagcg gaccaaccgg agaaaaagcg caaggaagca ctggatgcct
     3781 ttcaacggta tcgcgacaag ctatgggagg aatttcacga ctatgggtcg atggaagacg
     3841 gtcgcacgct ggggaccacg ctcctcgcgg atggccgtac cgatgttgct gtcccggtgc
     3901 tgctcaaaga gggctacgcc ttgagcgagg tggcacaagc cgcattggcg agactgtatg
     3961 aatcgggctc aggcacagcc ttagagccct acgataaaaa gattttggcc tgtttcgaaa
     4021 ccactgcggc ggaaggcttt gtccccgcaa agcgattatt ggcccgggtg tacggcaggg
     4081 gcatcggcgt tcctcaggac cttccgaagg ccaagggcat actgaaagga ctgcccaaga
     4141 atgaaatgaa tgccttcctg acggagatag gcggccggta gcgcatgcag atcggatggt
     4201 ccagattctg gcggctcttc atcaccactc ccctcctaca agcgtttgtc atcggtctgt
     4261 tggcgtcggt tctggccacg agtctttgga gcctggctcc cgtcacctct accaccctgg
     4321 attggactct ctatgatacc tgggtccggc atcgagtccc gatcgcgacc agtccgatct
     4381 tggccattgt cgtctccgat ccgcagagcg aagcccgatt gggaaccgta tt
//
LOCUS       DASTQY010000037         5949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_579987, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000037 DASTQY010000000
VERSION     DASTQY010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579987"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VFR82_01230"
     CDS             <1..692
                     /locus_tag="VFR82_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU4500249.1"
                     /translation="ITPQTRAILVNSPGNPSGKVFASDELEIVARIATEHDLLVFTDE
                     IYEYFVYDGRRHVSMASLRGMAERTITIGGYSKTFSITGWRIGYSVAASRWAQAIGAM
                     NDLLYVCAPTPLQAGVAAGVQELSGVFYRELAKEYQQKRDRFCDALVGAGLPPSVPQG
                     AYYVLADAMRLPGRTGKDRAMHVLKHAGIAGVPGEAFFAGSEGSRYIRFSFAKTDADL
                     EEACRRIARLS"
     gene            746..1216
                     /locus_tag="VFR82_01235"
     CDS             746..1216
                     /locus_tag="VFR82_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500250.1"
                     /translation="MIPSSWKTGLVSLPLVLSVACTSPLVMHEEVGQPSIPPTSGIPQ
                     DRRVLAGEWDYEDGAVVTLTLDEQGNGSYPWKDGRFVTHSLSDHVWQGMWIQKENDRE
                     GGFTVQFSPDFSDGEGQWWYTRIEDDRAPTQKGGTFRLTRKTSSSAANETFPAP"
     gene            1222..1536
                     /locus_tag="VFR82_01240"
     CDS             1222..1536
                     /locus_tag="VFR82_01240"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4500251.1"
                     /translation="MQERKHVRFAVQRPVSFRGDLLSGEGTILNISRQGCAILSDTAL
                     ETEAYIQLHLQLLEQEPPVHVDLAAVRWCTARKFGVEFIKMHHDVGERLKQFVQLLES
                     NG"
     gene            complement(1543..3333)
                     /locus_tag="VFR82_01245"
     CDS             complement(1543..3333)
                     /locus_tag="VFR82_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU4500252.1"
                     /translation="MPAGWAYGIKMIVQRNVNRTIDPRAFDLLINESFIRQGDLIKAV
                     QDSLQQGIELEDLLIQQYRVPKAAIGKALSRFFDCPYIPYDERMVIDPQLIKDLSHDY
                     LWKHAWVPLKANGSVLDVLIDDPHDIVRGHDIQRAFPGMTIRYAVALKGDIKRFLQST
                     IGMTDTGSIEDTLGELVHEARAEESLPGDVDEVDENDSAIVRLANQIIVEAYRQEASD
                     IHIEPYSDRKETAIRLRVDGTCSTYMRIPAAYRRALVSRIKIMSNLDIAERRKPQDGK
                     LRFRLNRDREIEVRVATMPTAGNNEDIVMRLLTAKEPMPLEAMEFAPSTLAGLLEVIE
                     KPHGILLCVGPTGSGKTTTLHAVLHRLNTDARKIWTAEDPIEITQEGLRQVQVLPKIG
                     FTFAAALRSFLRADPDVIMIGEMRDKETAEVAVEASLTGHLVLSTLHTNSAVETVTRM
                     LDLGCDPFNFADAMQGVLAKRLCKRICGHCKEMYHPTVQEYDKLVREYGSASWDTLDV
                     PPYGDAFTLSRGRGCDHCNHTGFKGRVALHELLSGSDELRNLIQNRAKTAEMISLAKK
                     AGMITLIQDGILKVLAGMTTYDQVRTVAMK"
     gene            complement(3515..4417)
                     /locus_tag="VFR82_01250"
     CDS             complement(3515..4417)
                     /locus_tag="VFR82_01250"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4500253.1"
                     /translation="MTPSLKIALLGTGLLGKAIAERLAAVGHRVIVFNRTQTKALPLE
                     AQGIAVVGSAEVAITQADLVLLLLTDAPAIHSVLFSPACSAALSGKTIVQMGTIGSNE
                     SLVIKREVERWGGTYCEAPVLGSLAEAKAGTLFVMVGGTEDQFATLSLLFRSLAREPR
                     LVGAVGKAAALKLALNQLIASETLAFALSLGLIRRAGISVDTFMAILRESALYAPTFD
                     KKLPRLLKRDYHHPNFSTRHLLKDVRLFVREAATRGVTTPPLEGVASLLEAAVARGLG
                     EADYSALYDLLDPPPSGSPPSSNN"
     gene            complement(4417..5217)
                     /locus_tag="VFR82_01255"
     CDS             complement(4417..5217)
                     /locus_tag="VFR82_01255"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEU4500254.1"
                     /translation="MEPELAQLSSTIRKAGARARDLAHKGFEIQTKKDRSPVTTADLE
                     VNRILREMQETHFPNDGWLSEESPDDPARLDKARVWIVDPIDGTKAFVNKLPEYCISV
                     GLIEGGVPVLAAIFNPSTDELFTAIRGQGVRLNGSPHTPETPVDTMSHVLVSPWEYRA
                     GRWANLDSQVRCRPMFSIANALAVVAAGRMQATLTIEPENEWDLAAGVLLVQESGGSV
                     ADAEGRPFTFNQATPRFRGVIAVAATAPADLHPMLQTHADRARTKKKR"
     gene            5402..>5949
                     /locus_tag="VFR82_01260"
     CDS             5402..>5949
                     /locus_tag="VFR82_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEU4500255.1"
                     /translation="MKLVQKRSKKTGLPPGTLVHIGERRTEQVTFTVFHYSAAGCEEL
                     QPHQANQLAPPADESVVWINVSGVHNVEVLEAFGKQFFLHPLLLEDIANTDQRPKLDD
                     YETYFFLVMKMLSLTDRKEIAVEQVCLVLGRNYVLSFQENGTDVFQPVRERLRGGKGR
                     LRQSGADYLLYALVDAIVDQYFA"
BASE COUNT         1227 a         1695 c         1756 g         1271 t
ORIGIN      
        1 cgatcacgcc gcaaacgagg gcgattctcg tcaattcacc gggtaacccc tccgggaaag
       61 tctttgcgag cgatgaactg gaaatcgtgg cacggatcgc cacggaacac gatctattgg
      121 ttttcaccga tgaaatttac gaatattttg tatacgacgg tcggcggcat gtcagcatgg
      181 cttcattacg aggtatggca gaaagaacca tcacgatcgg cggctattcc aaaacgttca
      241 gtatcacggg gtggcgcatc ggctacagcg tggccgcctc ccgatgggcg caggcgatcg
      301 gcgcgatgaa cgatctgctc tacgtctgcg ccccgacgcc gttgcaagcg ggcgtggcgg
      361 ccggggtaca agaactgtcc ggtgtctttt atcgggaact cgccaaggag taccaacaaa
      421 aacgtgaccg gttctgcgat gcactggtcg gggcgggtct gcccccctcg gtgccccaag
      481 gcgcctatta tgtgctcgcg gacgcgatga ggcttcccgg caggacagga aaagaccgcg
      541 ccatgcatgt gctcaagcat gccggcattg ccggagttcc gggcgaggcg tttttcgctg
      601 ggtctgaggg ttcacgatac attcgcttca gctttgccaa gacagatgca gatctggagg
      661 aagcctgccg ccgcattgcg cgtctttcct gagcgaggga atccttcact tgtcctgatc
      721 tcaacgccgt gatatgaaaa tacctatgat cccgtcatca tggaaaacag gactggtttc
      781 cttgccgttg gttttgagcg tggcctgcac cagtcccctc gtcatgcacg aagaggtcgg
      841 acagccctct atccctccta catcgggcat ccctcaagac cggcgtgttc tggccggaga
      901 gtgggattat gaagatggcg ccgtcgtcac actcacgctc gatgaacagg gaaacggctc
      961 gtatccctgg aaggacggcc ggttcgtgac gcattcgctg tccgatcacg tatggcaagg
     1021 gatgtggatt cagaaagaga acgatcgaga gggagggttc accgtgcagt tctctcctga
     1081 tttctccgac ggagaaggcc agtggtggta tacgaggatt gaagacgacc gggcgccgac
     1141 ccaaaaaggt ggcaccttcc gcctgacgag aaaaacttca tcctcagcag cgaatgagac
     1201 ctttcccgca ccgtgaggtc aatgcaggag cgcaagcacg tccggttcgc agtgcaacgg
     1261 ccggtctctt ttcgcggaga tttgctgtcc ggtgagggga ccatcttgaa catttcgcga
     1321 cagggctgcg ccattctttc cgacacggcg ctcgagacgg aagcctatat ccaactccat
     1381 ctgcagttgc tcgagcagga gccgccagtg catgtcgatt tggccgccgt gcggtggtgt
     1441 accgcgagga aattcggggt agagttcatc aagatgcatc acgatgtcgg agaacgtctc
     1501 aagcaatttg tgcagctgct ggagtcaaat ggctagcccg cactatttca tcgcgaccgt
     1561 tcgcacttgg tcatacgtcg tcatgccggc aaggaccttc aagatcccgt cctgaatcaa
     1621 cgtaatcatc cctgccttct tggccaggct gatcatctct gccgtcttcg cccggttctg
     1681 aatcaagttc ctcaactcat ccgatccact caacagttca tgcagggcga cgcgtccttt
     1741 gaaccccgtg tgattgcagt ggtcgcagcc gcgcccgcgg gacagcgtga aggcgtctcc
     1801 gtagggcggg acgtccaacg tgtcccaaga agcggagccg tactcgcgca ccagtttgtc
     1861 atactcctgc accgtcggat gatacatctc tttacaatgg ccacaaatcc gtttgcagag
     1921 acgtttggcc aggacccctt gcatcgcatc ggcaaagttg aacgggtcgc accctaagtc
     1981 gagcatgcgg gtcacggttt cgactgcact gttcgtgtgc aacgtgctca agaccagatg
     2041 gccggtcaat gaggcctcaa ccgccacttc ggcagtttcc ttatcccgca tttcgccgat
     2101 catgatgaca tctggatcgg cgcgaaggaa ggaccgtagg gcggccgcaa acgtgaatcc
     2161 gatcttgggc agcacctgca cctgcctcag tccttcctgc gtgatctcga tcggatcctc
     2221 ggccgtccat attttcctgg catccgtgtt caggcgatga aggactgcgt ggagcgtcgt
     2281 cgtctttccc gatcccgtcg ggccgacgca caagaggatc ccatgaggct tttcgatcac
     2341 ttccaaaaga ccggcgaggg tggacggcgc gaactccatg gcttcaagcg gcatgggttc
     2401 cttggcggtg agaagccgca tgacgatgtc ttcgttgttt cctgctgtcg gcatcgtggc
     2461 gactcgtact tcgatctccc tgtcccggtt cagccggaag cgcagcttgc cgtcctgcgg
     2521 ctttcttcgc tcggcaatgt cgaggttcga catgatcttg atgcgcgaca ccagggcccg
     2581 gcggtacgcg gccggaatgc gcatataggt cgaacacgta ccgtccacgc gaaggcgaat
     2641 cgccgtttcc ttgcgatcgg agtagggttc gatatggatg tctgaggctt cctggcggta
     2701 ggcctcgaca atgatctggt tggccaggcg gacgatggcc gagtcattct cgtcgacctc
     2761 atcgacatcg ccgggcaagg actcttccgc tcgtgcctcg tgcacgagtt cgccgagggt
     2821 gtcctcgatc gagccggtgt ccgtcatgcc gatcgtcgat tgcaggaagc gcttaatgtc
     2881 gcctttgagg gccactgcat agcggatggt catgccggga aaggcgcgtt ggatgtcatg
     2941 ccctctgacg atgtcgtgcg ggtcgtctat cagcacgtcg aggacgctgc cgttggcttt
     3001 taacggcacc cacgcgtgtt tccaaaggta gtcatggctc aagtctttga tgagttgagg
     3061 gtcaatgacc atacgttcat cgtagggaat gtagggacaa tcgaaaaacc tgctcaaggc
     3121 cttcccgatt gccgccttcg gaacgcgata ttgctggatg agcaaatctt ccagctcgat
     3181 gccctgctgg agggaatcct ggacggcttt gatcagatct ccttgccgga taaagctctc
     3241 attgatcaga agatcaaagg cgcgtggatc gatcgtcctg ttgacgtttc tttgcacgat
     3301 cattttgata ccgtacgccc agcctgccgg catgcgccga gcgaggccca cagtggagct
     3361 tggtcgagtc tgtacaccgg tttctggcgg tacgcccggg taaggaccaa ggatcggtat
     3421 tcagactcag tcattggtga tcaatcgagt atacgggtcc cgaagcgatc ctcaatgaat
     3481 aagtgaaaag aaaccgagac gcggcgtgag accatcagtt gttggaggaa ggcggcgaac
     3541 cggacggcgg aggatccagc aggtcataca acgccgaata atcggcttcg cctagccctc
     3601 gtgcgacggc agcctccaac agcgaagcca ctccttcgag gggaggggtc gtaacaccgc
     3661 gagtcgcggc ctccctcaca aagagcctga catctttgag caaatggcgg gtggagaaat
     3721 tcgggtggtg gtagtcgcgc ttgagcaaac ggggaagttt tttatcgaac gtcggggcat
     3781 acagcgcact ttcccgcaag atggccatga acgtatcgac ggaaatgcct gcccgtctga
     3841 tgagcccaag gctcaaggca aaagccaatg tctcggaggc aattagctgg ttgagcgcca
     3901 atttgagtgc ggcagccttg cccaccgccc cgacgagccg aggctcgcgg gcgagcgatc
     3961 ggaacagcag cgagagcgtg gcaaattgat cttcggtgcc tccgaccatg acgaacagcg
     4021 ttccggcttt ggcttcagcc aaactcccca ggacgggcgc ctcgcaatat gtccctcccc
     4081 atcgttccac ttcgcgtttg atcacaaggc tttcgttgga cccgatggtc cccatttgca
     4141 cgatcgtctt tcctgaaagc gccgcggagc aggcaggcga gaacaggact gaatgaatgg
     4201 ctggtgcatc agtaagaagg agcaggacaa ggtccgcttg agtgattgcg acctcggcag
     4261 acccgaccac ggcaatcccc tgcgcttcaa gtggaagggc cttggtctgc gtgcggttga
     4321 atacgattac cctgtggccc accgccgcca agcgttcggc gatggctttg ccaagcaaac
     4381 ccgttccgag taaggcgatc ttcagcgaag gagtcatcac cgcttctttt tcgttcgcgc
     4441 ccggtctgca tgcgtctgta gcatcggatg caaatcggct ggtgcggttg ccgcaaccgc
     4501 aatgacaccc cggaatcggg gagtcgcctg gttgaaagta aatgggcggc cctcggcatc
     4561 ggccaccgac ccgccgcttt cctgtaccaa gaggactccc gccgcgagat cccattcatt
     4621 ctccggttcg atcgtcagtg tcgcttgcat tcgcccggcc gccaccaccg ccagcgcatt
     4681 ggcaatcgaa aacatcggac ggcaacgcac ctggctatcc aaattggccc atcgaccggc
     4741 gcgatattcc caagggctga ccaggacgtg ggacatcgta tccactggag tctccggtgt
     4801 atgcggtgaa ccgttgaggc ggacaccttg gccgcggatg gccgtgaaca actcgtccgt
     4861 cgacggattg aaaatggccg ccagcaccgg aacgccgccc tcaatgagcc cgactgagat
     4921 gcaatattca ggaagtttgt tcacaaaggc cttcgtcccg tcgatcggat cgacgatcca
     4981 gaccctggct ttgtcgagcc gggccggatc atcaggggat tcctcggaaa gccacccgtc
     5041 atttgggaaa tgcgtttctt gcatctcgcg gaggatccgg tttacttcga gatcggcggt
     5101 agtgactggc gagcgatctt tcttggtctg aatttcgaag cccttgtggg ccaggtccct
     5161 ggccctggcc ccggccttcc tgatggtgct gctgagttgg gcgagttctg gttccatggt
     5221 gctgtgagtg atgagtcttg gtcagagtac caggtcttgg ggtatcggaa aagcagaacc
     5281 tcgagccaga ttcctcttga ccgctcgttc gaacctgcgt acaagctggc ccagcttgac
     5341 gggagatggc cgcgggagtc tgctcgcatg gcacgacgaa cagcctgaca aaagacaacg
     5401 tatgaagctg gttcaaaagc gctcaaaaaa aacaggtctg ccgcccggga cgcttgtcca
     5461 catcggcgag cggaggactg aacaagtcac gttcaccgtc tttcactaca gcgcagccgg
     5521 ctgcgaggaa ctgcaacctc accaagccaa ccagctggcg ccgcccgccg acgagtcggt
     5581 cgtctggatc aacgtcagcg gggtacataa cgtcgaggtg ctcgaggcgt tcggcaagca
     5641 gttttttctg cacccgctgt tgttggaaga catcgccaac accgaccagc ggccaaagct
     5701 cgacgactat gaaacgtatt ttttcctcgt catgaagatg ctgtccttga cggatcggaa
     5761 agagatcgcc gtggagcagg tctgtctggt gttgggacga aattacgtcc tctcgttcca
     5821 ggaaaatggg accgatgtct ttcagccggt ccgggagcgt ctccgaggag ggaaaggacg
     5881 gctgcgtcaa tcaggcgccg actatctgtt gtatgccttg gtggatgcga tcgtcgatca
     5941 atatttcgc
//
LOCUS       DASTQY010000038         3371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1386106, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000038 DASTQY010000000
VERSION     DASTQY010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386106"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1091)
                     /gene="leuB"
                     /locus_tag="VFR82_01265"
     CDS             complement(18..1091)
                     /gene="leuB"
                     /locus_tag="VFR82_01265"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU4500256.1"
                     /translation="MKAKIAVLAGDGVGREIVPEAVKVLRAVGERCGHHFEFTSGDIG
                     GQAIDKVGVPLPQETLTVSKQSDAVLLGAVGGPKWEGLDYSLRPERALLGIREALGLY
                     ANLRPAKLYPNLADASTLKKEVVEGIDILVVRELTGGIYFGKPKGIEKLPNGSERGVN
                     TEVYTTEEIRRIAIVAFDAARKRRKKLTSVDKANVLESSELWRRVVVDVHKDYPDVSL
                     SHMYVDNAAMQLVRNPRQFDVMLCNNMFGDILSDEAAMLTGSIGMLPSASFGAQVGLF
                     EPIHGSAPDIAGKNIANPIATIASAAMMLSYAFKMEKEAELIEQAIVKALDLGYRTKD
                     IQSPGSRVVGTTEMGDVIMRSLG"
     gene            complement(1160..1522)
                     /locus_tag="VFR82_01270"
     CDS             complement(1160..1522)
                     /locus_tag="VFR82_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU4500257.1"
                     /translation="MFKVTPADRPEFLAGDHTRLREVFHPAKHPLKLGYSLAHGTLGP
                     GQRSKWHVLSSSEVYYFLTGHGRFMVDEQVTTVEAGTTLYVPPGSKQSLENIGIDAIE
                     FLCVVDPAWKTEDERILE"
     gene            complement(1583..3130)
                     /locus_tag="VFR82_01275"
     CDS             complement(1583..3130)
                     /locus_tag="VFR82_01275"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU4500258.1"
                     /translation="MTRMIRIFDTTLRDGEQSPGASMNVEEKVMVAKQLARLGVDIIE
                     AGFAYSSPGDFEAVRRIAQEVEGPTICSLARARPEDIDRAWEALKGAPKVRIHTFLST
                     SDIHLKHQFRMTREEAKKRAVEMVQRARGYVEDVEFSPMDASRSDPAYLYEVIEAVIE
                     AGAGTVNIPDTVGYAIPQEFGKLIKGIRERVTNSDKAVISVHCHNDLGLAVANSLAAV
                     MEGAGQVECTINGIGERAGNTSLEEIAMGLRTRKDLYQADTRIHTEEISKTSRLVSKI
                     TGMVVQPNKAIVGANAFAHTSGIHQDGLLKDKTTYEIMRPESVGLVESKMVMGKLSGR
                     HAFRQRLEELGYKLTEEEVNHAFDRFKKLADHKKEIYEEDLEVIVSEELAKMADRITL
                     KSFSVSSGTDQVPTATVELEIDGKSLSQRGTGDGPVDAVYRTIAAITQTKSKLLMYVV
                     KAITGGTDAQGEVSVRVQEDGRTVSGHGADTDIITASARAYLNALNKLAYLATKQAQG
                     EQKVNLI"
BASE COUNT          632 a         1052 c          875 g          812 t
ORIGIN      
        1 agaaacgctc atggtcatca gccgaggctc cgcatgatga cgtcgcccat ttcagtcgtc
       61 ccgaccaccc tgctacccgg actctgaatg tctttcgtcc gataacccag atccaaggcc
      121 ttgacgatcg cctgctcaat cagctcggct tccttctcca ttttgaatgc gtatgacagc
      181 atcatggctg ccgaggcaat cgtcgcaatc gggttggcga tgttcttgcc tgcgatgtcg
      241 ggcgcgctgc catggatcgg ctcgaatagg ccgacctgcg ctccgaagct ggcggacggc
      301 agcatgccga tcgagcccgt caacatcgcg gcttcatcgc tgagaatgtc accgaacatg
      361 ttgttgcaga gcatgacatc gaattgccga ggattgcgca caagttgcat ggccgcattg
      421 tcaacataca tatggctgag actgacgtct ggataatctt tgtggacgtc caccacgaca
      481 cgacgccata gctcggagga ttccagcaca ttggccttgt ctaccgaggt cagcttcttc
      541 cggcgctttc gagcggcgtc gaacgccacg atggcgatcc gtcggatttc ctccgttgta
      601 tacacttcgg tattgacccc tcgctcgctg ccattcggca gcttctcaat ccccttcggc
      661 ttgccgaaat agattccgcc ggttaattct cggacgacga ggatatcaat gccttccacg
      721 acctcttttt ttaacgtgga ggcgtcggcg aggttgggat acagtttggc cggccgcaga
      781 ttggcataga ggccgagcgc ttcccgaatg cccagcaacg ctcgctcggg cctcagactg
      841 taatccaatc cttcccactt cggtcccccg accgccccta gcagcacggc gtcgctttgt
      901 ttggacaccg tcagcgtctc ttgaggtaac ggtactccca ctttatcgat ggcctgacca
      961 ccgatgtcgc cgctcgtaaa ctcaaagtga tgcccgcacc gttcaccgac cgccctcaac
     1021 accttgactg cttcgggaac aatctctcga ccgacgccgt cgcctgctag taccgcgatt
     1081 ttggctttca cgctacctcc gccgttggct tccgcttcat gacactcccc gtaccgtcga
     1141 ggcttccctc acgtcggcgt cattccaata ttcgctcgtc ttcagttttc caagcggggt
     1201 cgacgacgca gagaaactcg atggcgtcaa ttcctatatt ctcgagcgat tgtttactgc
     1261 cgggcggtac gtagagggta gtgccggctt ccacggtcgt gacctgctcg tcaaccatga
     1321 accgtccatg tccggtcaga aaataataca cttctgatga actcaggaca tgccatttcg
     1381 aacgctgacc gggccctaac gtcccgtgtg cgaggctata tccaagtttc agcggatgct
     1441 tggccggatg gaacacttcg cgaagccgcg tatggtcgcc ggcgaggaac tcgggccgat
     1501 cagccggcgt gaccttgaac acagcagccc ccttcgccct atcaggcttg gtaaaggggg
     1561 cactctaccg tgcgaggttt catcaaatca agttcacctt ctgctccccc tgtgcctgtt
     1621 tcgtagccaa atacgccagc ttgttcaagg cattcaggta agcccttgcc gaggcggtaa
     1681 tgatatcggt gtccgcgcca tggcccgaca cggttcgtcc atcctcctga actctgacgg
     1741 atacttcgcc ttgggcatct gtcccgccgg tgatcgcctt gacgacgtac atgagcaact
     1801 tgcttttcgt ctgtgtgatg gcggcgatgg tccggtagac agcatcgacg gggccatcgc
     1861 ctgtcccccg ttggctgagc gacttgccgt cgatttccaa ttcgaccgtg gcggtcggca
     1921 cctgatcggt tccgctgctc acgctaaagg atttcagtgt gatccgatcg gccatcttgg
     1981 ccaattcctc ggagacgatc acttccagat cttcttcgta gatttctttc ttatgatcgg
     2041 cgagtttctt aaagcgatcg aaggcatggt tcacctcttc ctctgtcaac ttgtacccca
     2101 attcttcgag gcgctggcgg aacgcatggc gtcccgaaag cttgcccatg accatcttgc
     2161 tctcgaccaa cccgaccgac tcgggccgca tgatctcata cgtcgttttg tccttcagca
     2221 gtccgtcttg atggatcccg gacgtatggg caaacgcgtt ggctcccacg atcgccttgt
     2281 ttggctgcac caccatgccg gtaattttgc tgaccagtcg actcgtcttg gagatttctt
     2341 ccgtgtgtat tctggtatcg gcttgataga ggtccttccg cgtgcgcaag cccatcgcaa
     2401 tctcttccaa cgaggtattg ccggcgcgct cgccaatccc gttgatcgtg cactcgacct
     2461 ggccggcgcc ttccatgacg gccgcgagac tattggccac ggccagccct aaatcgttgt
     2521 gacaatgcac tgagatcacc gccttgtcgc tattggttac ccgctccctg atccctttga
     2581 tcagctttcc gaattcctgg gggatggcat atcccacggt gtcagggata ttgaccgtcc
     2641 ctgcgcccgc ctcgatgacg gcttcgatca cctcgtacag ataggcggga tcagagcggc
     2701 tcgcgtccat cggtgaaaac tcgacgtctt ccacgtagcc tctcgcccgc tgtaccattt
     2761 cgacggcgcg cttcttggct tcttcacgcg tcatgcgaaa ttgatgcttc agatggatgt
     2821 ccgacgtcga aaggaacgtg tgtatgcgga ccttaggcgc acctttgagg gcttcccagg
     2881 cacggtcgat gtcctcgggg cgagcccgtg cgaggctgca gatcgtcgga ccttccacct
     2941 cttgggcaat gcgccgtacc gcttcgaaat ccccggggga actataggca aatccggcct
     3001 cgataatgtc cacgccaagc ctggccagct gtttcgccac catcactttt tcttccacgt
     3061 tcatactcgc gccgggggat tgttccccgt cacgcaatgt ggtgtcgaaa attctgatca
     3121 tccgtgtcat gttctcaccc cttctgttct gagaaatcgc tcaaaacgtc ttccagcaag
     3181 gccgcatgga gggaggttcg agactgagaa cgccgatggg aggcgtcttc gacgatctca
     3241 aaatgcaaaa gcctccatcc cttactccag ggacggaggc ttcgaagact ccgtggtacc
     3301 accctgattc ggttcggaca tcaccgtcgt ggtccgatac ccttcattcg cccgtcacga
     3361 gggcaaaacg g
//
LOCUS       DASTQY010000039         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1383952, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000039 DASTQY010000000
VERSION     DASTQY010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383952"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /gene="pyk"
                     /locus_tag="VFR82_01280"
     CDS             <1..1085
                     /gene="pyk"
                     /locus_tag="VFR82_01280"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU4500259.1"
                     /translation="LINLEVLEKEERHMRCRVLDGGLLKSKRHVNLPGIRVNLPAITQ
                     KDTKDILFGLERDVDFIALSFVREAADIQQLKALMGEKVGRVKIVAKIEDQEGVRNLE
                     EIIKESDGIMVARGDLGVEINIEDLPNVQRTIVQRCAEHGKRVIVATHLLESMIHNPH
                     PTRAEVTDVANAIYEEADAVMLSGETTVGKYPVKCVEYLRKIALKSETIPGLRFAKQL
                     RDTGNKQQLAAAAVQLAEGVNAKGIVVITRRGLMADLVANCRPFSTNIYAFTNMSQPR
                     RTMMLNRGVFPFKIDFSSDPEKTLQTAFRILKEREQFQVGDKVVIISDVLAQQRVDSI
                     QIRDVPSDDVSPDTSIPTDAGSSSVG"
     gene            1589..2278
                     /locus_tag="VFR82_01285"
     CDS             1589..2278
                     /locus_tag="VFR82_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500260.1"
                     /translation="MTIKESGSSAHSTFSLLILVLFLFGWFVLPSLKLSRSQAYAAGG
                     GQTKIQRISTQFIAALGDPGATSGSGAQSWGLWPLDPGPRGVELDNYTRLKDTGGVAP
                     ARWKFDGTDWWLEEHGLIMEQPTFPLPPGKYLVTGARDVTAVLTIHPADRNGDRRWEL
                     DKGATLYDVTHLACRSARYTPAAVGGSCSPANVQKAAFPVAPGRAMPPVAGCTKQDYA
                     VLIVIGVGVED"
     gene            complement(2503..>2714)
                     /locus_tag="VFR82_01290"
     CDS             complement(2503..>2714)
                     /locus_tag="VFR82_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU4500261.1"
                     /translation="KFTGQSRGFGFVEMSSDSEAKAAITALNGSDMGGRSLTVNEAKP
                     MEQRSGGGGFGGGGNRGPGGKRDRW"
BASE COUNT          619 a          739 c          791 g          565 t
ORIGIN      
        1 ggctgatcaa cttggaggtg ttggagaagg aagagcgcca catgcggtgt cgtgtcttgg
       61 acggtggact cttgaaaagt aagcgccacg tcaatctccc ggggatccgt gtgaatctgc
      121 cggcgatcac gcaaaaggac accaaggata tcctgtttgg cctggaacgg gatgtggact
      181 tcatcgcgct ttcgttcgtc cgtgaagccg cggatattca gcaactcaag gcactcatgg
      241 gtgaaaaggt cgggagggtg aagattgttg caaagatcga ggaccaggaa ggcgtgcgta
      301 acctggagga gatcatcaag gaatcggatg gcattatggt cgcgcgtggc gatctgggag
      361 tagaaatcaa catcgaggat ctcccgaatg tccagcgcac gatcgtacaa cgttgtgccg
      421 agcacgggaa gcgagtgatc gtcgcaaccc atctgctcga atccatgatc cacaatccgc
      481 accctacacg cgccgaggtc accgacgtcg ctaacgcaat ctatgaagaa gccgatgccg
      541 tcatgctgtc aggagaaacc acggttggga agtatccggt gaaatgtgtc gaatacctgc
      601 gcaagattgc gctgaaatcc gagaccattc ccggattgcg atttgcgaaa caattgcgcg
      661 atacgggcaa taaacaacaa ttggcggcgg cggccgttca gctcgccgaa ggggtcaacg
      721 ccaagggcat cgtcgtgatt acgagacggg gactcatggc cgatctcgtc gccaactgcc
      781 gtccgttctc aaccaacatc tacgccttta ccaacatgag ccaaccgcga cggaccatga
      841 tgctcaaccg tggtgtcttt ccgttcaaga ttgatttcag ttcggaccct gaaaagacac
      901 tacaaacggc gttccggatc ttgaaggagc gagaacagtt tcaggttggg gacaaggtcg
      961 tgatcatctc ggacgtcctg gcccagcaac gggtcgactc gattcagatt cgtgacgttc
     1021 cttcggacga cgtctcgccc gacacatcca tcccaaccga tgcaggctcc tcatcggtcg
     1081 gatagtcttt tggtaaaagg gggacattcc ccgagtggtg tgagtctctt cgggacaaga
     1141 catgaaaaga gttatcattc tgatgagcat cgttttcaca gcgtgttctg caacagttcc
     1201 tgcgactatg aggatcgaaa gaaatcaaat tctccgagga tctgcgagcg ggaaggtggg
     1261 gagggatgcg gttatttccg tgaaaactat cgcggggctg tcctgcgcgg ggaagatgtt
     1321 tgttcctgtc accgaaacca ctactgaagg cacaattcaa tgtagcgaca agcggaaagg
     1381 gaattttatt gcgaatggta agggcgactc gtgggcggga gaggggaaac tgaacgatgg
     1441 atcgaaattc ttaattttgc ttggaacgaa taggacagcg ctagagtact aacacgtgct
     1501 agcctgtctg aattgttccg gcccctccaa aacgtgtttc cttcggatga gtccagcccc
     1561 gctgatcggt tctgacgcag gagcaaatat gaccataaaa gagagtggct cttctgccca
     1621 ttctacattt tctctgctca tcctggttct ctttctgttc ggttggttcg tgctcccctc
     1681 actcaagctg agtcgaagcc aggcctacgc cgcaggcgga ggtcagacga aaatccagcg
     1741 catatcgaca cagttcatcg cggcgttggg cgatccaggt gcgacctccg ggagcggcgc
     1801 gcaatcgtgg ggtctgtggc ctctggaccc tggtcctcgg ggcgtcgaac tggacaacta
     1861 tacacggttg aaggacacag gtggagttgc cccggcgcgc tggaaattcg acggcacgga
     1921 ctggtggctg gaggagcacg gactgatcat ggagcagcca acctttccgc tccctcccgg
     1981 caaatacctg gtcacgggcg cccgcgacgt gacggctgtg ctgaccattc atccggccga
     2041 caggaatggc gacaggcgct gggagctcga taaaggcgcg acgctctacg acgtgacgca
     2101 cctggcctgc cgctccgcac gctatacacc ggcagcggtt ggtggttcgt gctccccggc
     2161 aaacgtgcag aaggctgcgt tcccagttgc gcctgggaga gcgatgcctc cggtcgcggg
     2221 ctgcacgaag caggactatg ccgttctaat cgtgatcggc gtgggggtgg aagactgaac
     2281 aacagtgacg atcggctaaa ttaaaaaagg tgtcaggaac aatttcgcgg cacgtggcgt
     2341 ccggcttgtt cctagtctct ctgccccttt aacgatcatc gcacctcgca ccgcgataga
     2401 cacgtgccca aggcatggca catcgcggaa tggagtctgt gcacaaaaaa agggtgccat
     2461 gctttcatgg caccctctcg cgtgttcgat cgagtgaacc gcttaccagc gatcgcgctt
     2521 tccaccgggg ccacggttac caccgccacc gaatccaccg ccaccggaac gctgctccat
     2581 cggtttcgct tcattgaccg ttaacgagcg gccacccatg tccgagccat tgagcgccgt
     2641 gatcgcggcc ttcgcctcag aatccgagga catctccacg aagccaaatc cgcgggactg
     2701 tcccgtgaat ttat
//
LOCUS       DASTQY010000040         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1181504, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000040 DASTQY010000000
VERSION     DASTQY010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181504"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..1250
                     /locus_tag="VFR82_01295"
     CDS             372..1250
                     /locus_tag="VFR82_01295"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU4500262.1"
                     /translation="MKHHDETLRPPAGPFGPASMTRRGFVTATLAAGFALAVRPVSAA
                     TITTDETGLTTGAIQIRTRDGQIPAYRARPEKGRALPVVLVVQEIFGVHEHIKDICRR
                     LAKLGYLAIAPELYARQGDVSKMTDIDEIRKVVSQVADAQVLADLDAAATWAKESGEG
                     NVERLGVTGFCWGGRIVWLYAAQNAQVKAGVAWYGRLVGAPTTLQPKHPIDGASSIKA
                     PVLGLYGAEDQGIPLDTVEQMKTALRAAGATSEIVVYPNVGHAFYADYRPSYRKEAAE
                     DGWKRLQEWLKRHGVS"
     gene            complement(1915..2139)
                     /locus_tag="VFR82_01300"
     CDS             complement(1915..2139)
                     /locus_tag="VFR82_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500263.1"
                     /translation="MTRFFTLFAMGLLASPTLALAAEHNAGYRGIGYIYFSFIAAVLI
                     YGVYDSFGKMAMYVMTPIILVSLFLMLPPV"
BASE COUNT          568 a          659 c          727 g          480 t
ORIGIN      
        1 atgcgcttga gcagcatccg ccctggccgc aggtcagcgg ggatgaaaag agattggctg
       61 gcagcgtggg tcgaccgcag catcacacac caaactaata cactatagat actacttact
      121 cgagactacc ggttcctagg cagctccctc tcctaggatc tttccgagtc ccgtgcggtg
      181 gtgagcatgg tcatcttgag tttattgcct ggcgggctcg tgcgcgctcg tggccatggt
      241 cttctcgtgg gcggacctcc ggcacagcgg catccaccgg cgacatcgga tcggaaatga
      301 agaggcagga cgacacatgg tctcttgtgg taatcccaac gtcagtccat ctttttagcg
      361 ggggtagaac gatgaaacat cacgacgaga ccttgagacc gccagccgga cccttcggcc
      421 ccgccagcat gacgcgacgc ggatttgtca ccgcgacgct ggcggccggg tttgccttag
      481 ctgtgcgccc agtctcagcc gcaaccatca cgacggacga gacaggtctg acgaccggcg
      541 cgatccagat tcggacacgt gacggccaga ttcccgctta tcgagcaagg ccggagaaag
      601 gccgtgcgtt gccggtggtg ttggtggtcc aggagatttt tggcgtgcac gaacacatca
      661 aggacatttg ccgccggctg gccaagctcg gttatctggc aatcgcaccc gagctctatg
      721 ctcgtcaagg tgatgtctcg aagatgactg acatcgatga gatccgcaaa gtggtctcgc
      781 aggtcgccga tgcgcaggtc ctggccgatc tcgatgccgc cgcgacctgg gccaaggagt
      841 caggtgaagg taacgtcgag cgacttggcg tcacgggctt ctgctgggga ggaagaatcg
      901 tctggctcta tgcggcccag aatgctcaag tcaaagccgg agtggcctgg tacgggagac
      961 tggtcggcgc tcccacgacc ctgcagccca aacaccccat cgatggtgcc tcgtcgatca
     1021 aagcgccggt cctgggactg tacggggcgg aggatcaagg cattccgctc gacacggtcg
     1081 aacaaatgaa gacggcgctt cgggcagcag gcgccacatc ggaaatcgtg gtctacccga
     1141 acgtcggcca tgccttctac gcggactatc ggccgagcta cagaaaagaa gccgcagagg
     1201 acggatggaa acggctacag gaatggctga agcgtcacgg ggtatcgtag tccgatcgga
     1261 cagctcaggg aaaaagtttc ctcctccatg agaaagccag ccagatggag ccccaaatgg
     1321 tacgcgttcg actggtctgc tagggggaaa tggtcttgcc tgtgggcctt acgcgttgca
     1381 tgaaatccgt atgacatcct gaacaatacg tgtgggtcac cgggtttccc atgagcctga
     1441 tcccatacat ctgctcatag gcccgcttcg tgacccaccg atctgggtcc ccggcaaatg
     1501 tctttcgtgc tcgcaccagt ccgcacacac aacacttggc gattagagag gtctcaacac
     1561 gtaggccccc acggttgagg ccttgtcgga atgtagccat acatcctccc gatgtcaggt
     1621 ggtctccttc tcctattgaa agcgtagctg gtgggtggac cctcttggca cgtaaatccc
     1681 ctagtttaag gcgtaggatt ccccgagttt ctccaggtga aaatcctagt tttcctcttt
     1741 ttcggcagag gcaggcattt tgaagcgagt aggcggggat caaatgggaa acaactggga
     1801 catcggtcag cgcacaaggc cgctgcgcgt tcatgagccg gcgagcggaa gagaaagggt
     1861 tgttacagtt tctttgagcc gacctgaaag atcccagttc gcgtcggatg caaactagac
     1921 aggcggcaac ataagaaaaa gactcaccag gatgatcggt gtcatcacat acatggccat
     1981 tttgccgaat gaatcgtaca caccgtagat caaaacggcg gcaatgaagc tgaaatagat
     2041 gtaaccgatt ccccggtagc cggcgttatg ttcagcggcc aaggctagtg ttggcgaggc
     2101 gagtagtccc attgcaaaga gcgtgaaaaa tcgtgtcatg gaagactcct tggttaaggt
     2161 aagtgtacgt agcggatcgg ttcgcgaggg agcggacaga acaacgccct gccggtgaac
     2221 cgcacccggc agggcgtgaa aatgaatggc ggaaaatgag aaggcgccgc gccgatcaac
     2281 acgacagcac agaacaccta tataagaaat aggcattcat gctgcgcttt ccgacatagg
     2341 aagtcaaatg atgggaggag aggcctgcat actctggcga ccttcgaggt gacggatcaa
     2401 atcgatccgt ttcatgaaat cgaggcaaga ggct
//
LOCUS       DASTQY010000041        10062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1786755, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000041 DASTQY010000000
VERSION     DASTQY010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10062
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786755"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(437..1498)
                     /locus_tag="VFR82_01305"
     CDS             complement(437..1498)
                     /locus_tag="VFR82_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500264.1"
                     /translation="MRERRWETRGPMTFTQVLAVGERLAALGLKPAVAAKDVICYVEE
                     WSVEAPEDFDSLDAWSTEDVTLVHVRDGWRGDFFLLAGAYHTTYLRYQNVGTYCSISH
                     PWRIRERMKLHENRSMLWLGFRHAHSFLRVRLHTREVVTPGETRDDARREQWLDERRT
                     AFLEAITTVGLPIETAIEKGQLVLRPNDPTVPFFCSWPDAFGPCQFEYNTSDAYEFLV
                     PASQLVATYAPEPAGVRAYLTGFSEEALGEFARIEPAARFAYRCSVHCPLDDLPEIRS
                     AIEPDGRLYATLCEFQTQELIPDGEEASAIIGVVGSQTGFAIEARLNKAPLKEDLMAG
                     WLERLIGEPVVYAPLPAFV"
     gene            complement(1521..2168)
                     /locus_tag="VFR82_01310"
     CDS             complement(1521..2168)
                     /locus_tag="VFR82_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500265.1"
                     /translation="MIHSLMRVGAGILLAVGLMTTSGCGGSGETLHLDVLPQQQPVQV
                     AEPEAVKIVIEPFEDRRADKHRIGMRTHLWGGVTYFNVTGEKPGEVYAQALADRLRAS
                     GWLDRSWDVRVAPAGSAPNADIVISGQLFEFAANAKSRLFSTYLTTSNKLTITARNNV
                     DRSSTSRSLEGAQTDTVVWFSEDDVKKLMTATVKDTLDRYLADTTISQRAVRPSR"
     gene            complement(2319..2936)
                     /locus_tag="VFR82_01315"
     CDS             complement(2319..2936)
                     /locus_tag="VFR82_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500266.1"
                     /translation="MTNALSFTDEETALLIDAQFFQKKAKISEKIRVQLEKTGEALKA
                     ELSRSQLPALPHDGFSAQFVKGEHLENFPYQYLDCPKYFHGPEKRTFRTLVWWGHHVA
                     CAWLLEGSLTKQYKARLVDRFHTIAGRHLELSLAPTLWEWKRGEGYTLPITHDRKAQT
                     AAVLAERTFLKIVRFIPFDDARLRSGDLAGLSLEAFRAMLPIVSL"
     gene            complement(3104..3478)
                     /locus_tag="VFR82_01320"
     CDS             complement(3104..3478)
                     /locus_tag="VFR82_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500267.1"
                     /translation="MGQVFHSGAFLQQCWSVHPLCVSVRRITGGNQLILACTSCRFAH
                     YLMLGDVAVRTSASEQTEPSTGESDAQLPSQVRFAACLEAHAAALTLREMDVFEDLAV
                     IRCAECRRLYVLTVTAFETHQK"
     gene            complement(3491..3772)
                     /locus_tag="VFR82_01325"
     CDS             complement(3491..3772)
                     /locus_tag="VFR82_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500268.1"
                     /translation="MFAQVVRYGLAPAILSISGFFTANFLASGLYTWPRTSRILALCL
                     TLLVLSYEFVYKEQCARTGSKEQARAALFYSCAIPYLGGMVLMFALWNW"
     gene            4331..5221
                     /locus_tag="VFR82_01330"
     CDS             4331..5221
                     /locus_tag="VFR82_01330"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEU4500269.1"
                     /translation="MNIKDYLEQKRIEVDRFLDEVTPPAGTPPVTLHDSMRYSLLGGG
                     KRIRPILTIASAEALGTNPPGLMAAACSLELIHTYSLIHDDLPSMDNDDFRRGKPTNH
                     KVYGEAMAILAGDALLTMAFDLISRPDLMKGCDPVRQARIIQELAFGSGNMGMVGGQV
                     LDIQAENQDIDLPTLQNIHKHKTGMLIRAAVRMGAIAGGANDRQLDDLTGYAEGIGLA
                     FQIADDVLNVTGTREELGKNPNTDAERGKKTYPTFYGVEGARQFADACVTRAIDRLSS
                     FGSTADPLREIARYITSRRN"
     gene            5309..6292
                     /gene="hpnA"
                     /locus_tag="VFR82_01335"
     CDS             5309..6292
                     /gene="hpnA"
                     /locus_tag="VFR82_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HEU4500270.1"
                     /translation="MKALVTGATGFVGGAVARALVGAGVDVRVLIRSNSDLQNLSGLS
                     VERVEGDLRDPASLRPALAGCHHLYHVAAHYALWAKDPAIFYDVNVTGTRNVLSAAKL
                     AGVERTVYCSTIGAIGLPPGGGLGSEETPVSLDQMAGHYKRSKYLAEQEVLKFAHEGL
                     PVVIVNPSAPVGAGDVRPTPTGQVIVDFMKGRMPAYIETGMNIVDVEDVAAGHLLAME
                     KGRQGERYILGCKNLMLREVFDILSGLTGVKAPSLRLPRWTVLPLAYANQWVANVTGH
                     PPRIPLEGVKMAKYRMHYDCGKAIRELGIPQTPPEVALEKAVRWFRSHGYA"
     gene            6398..7015
                     /locus_tag="VFR82_01340"
     CDS             6398..7015
                     /locus_tag="VFR82_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU4500271.1"
                     /translation="MKTKLLGAAVLIVIAGLGILSWVGYQLFTTGFSAKTEPHALEIW
                     MARQIRHLAIPIDKRNLTNPVPLTAEVRREGRAHFADHCAVCHANDGSGQTPIGKNVY
                     PKAPDLRLRETQSMSDGELFWVIHNGIRFTAMPAWGAGEPEKDLDSWKLVHFMRHLPQ
                     LTQEELDEMKTLNPKTPNELLEEAAFERFLEGADAGGKSLEGHHH"
     gene            7048..7410
                     /locus_tag="VFR82_01345"
     CDS             7048..7410
                     /locus_tag="VFR82_01345"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEU4500272.1"
                     /translation="MFHSLILAVLCVMLPTTALAHGTGQHVLGTVVAIDSKHLEVKTQ
                     KGTTVEVLINKQTRFKEKGNPKGANLPSVGDRVVVEAIKDDKALTATEVHFSSFKKPA
                     QEPAPLPQQTPAAGTPAP"
     gene            7422..7880
                     /locus_tag="VFR82_01350"
     CDS             7422..7880
                     /locus_tag="VFR82_01350"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU4500273.1"
                     /translation="MRIVHLSQQMQSAPFSGGFHGAMVRLLITGIAVFLAVATVPGLE
                     ADSFGAGVAAVLVLTLLNLLLRPLLFILTLPLIVLSLGLFLVVLNALLLELTAYMVKG
                     FTVSGFWPAVGGALVISVVTSVLNLFTGDYRQVDTRTTQPREPKVINPPE"
     gene            8129..9949
                     /locus_tag="VFR82_01355"
     CDS             8129..9949
                     /locus_tag="VFR82_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4500274.1"
                     /translation="MTPAIPQSKPPSETQLQRTLNSVLNDIPVDSALAAILHRENGPL
                     VGHATRGFTARDVQAILRTLSAPALITAGAQDQDGGRTMRLRMITPGAKSLLGVPLRY
                     RNRTYGFLVIGRKESAAFTKKERGLMDQASEDVTKALDREKLFDMNVVLSRPLVNQEQ
                     VSVAIPSPPLLSAPVSQLTPQLQENITALLTDAAETVTYDRSWVCHYDPIAGSVEVLG
                     MAGDLKGEHKDGKKDMRPGHRLALDSSAAGWAIRHRKPRVDHDLASTQGRFLDHKQLF
                     KDRFQSSLVFPFFVRGQVGGTVTLASREADRYQPTDARTLEPLILKLADLLQAPSAVE
                     AAPAPVVDEGGTSAAPAQPAVISQEPIIRKQERQAAIGEFSAFLATEVREPLGSIRAQ
                     LEEVTGEGVLDFDPQTRVENAMRDLMRVEAILNEILDFAKPLELNRRLVRIPEVIESA
                     LTVVANDLEITRIQVGKEYAPILAPVRGDEAKLQQVFLSIFKNACEAMSPGGRLTIQV
                     AQHRAGRGIEDHILIKNDGAPIPPEIVDKVFEPFFTTKKAGSGLGLATVKKIVEEHGG
                     SIAIGSAPGEGTTVTIRLPGVSRGPSFRHRGRGRRPPHRR"
BASE COUNT         2229 a         2913 c         2900 g         2020 t
ORIGIN      
        1 gagggtgtca taggtcaccc cggacaggaa aaacacggcg ggcatgaacg gcttggccag
       61 cagcgactgg accctggaaa gcgggcgcat ggttctactg tgcggaatct gatgactttg
      121 agcaacgaaa atgtgacttg cgataggatc ttcgcagact gctcaaacca gttgatgctc
      181 tcacccgccc aaccccgatg cgccgacgcg tctctccaca agcgcggccg caacgaggtc
      241 cgtgacgcgc agaatatgaa cgtcatgctt gcggacaggt gcgagttggt gacgcctcca
      301 ctatcttagc cccgagacat actctttgca gctacgccgc aggtgagaag ggacgtgaga
      361 acgaaccgtg aggaagggcg catcttggcg ccagggctgg gtgggtgcga agagcgactt
      421 gtttcagcag tcttactcag acgaaggcgg gaagcggcgc atagacgaca ggctctccga
      481 tcagtcgttc cagccaccca gccatcaggt cttctttcag cggcgccttg ttcaaccgcg
      541 cctcgatcgc aaaacccgtc tgtgatccga ccacgccgat aatcgcggag gcctcttcgc
      601 catcaggaat aagctcctgg gtctggaact cacagagcgt ggcatacaga cgcccgtccg
      661 gttcaatggc cgatcggatc tccggcaggt catcgagcgg acaatgaacg gaacaccgat
      721 aggcaaaacg ggccgcgggt tcaatgcggg caaattctcc aagcgcctct tcggaaaatc
      781 ccgtcaaata ggcgcgcacc cctgccggtt caggcgcgta ggtcgcgacc agctggctgg
      841 ccggaacgag aaactcgtat gcgtcggagg tgttgtattc gaactgacac ggcccgaagg
      901 catccggcca tgagcagaaa aatggaaccg ttgggtcgtt cggacgaagt accagctgac
      961 ctttttcaat ggccgtttca atgggcaacc cgacggtggt aatggcttca agaaaagcgg
     1021 tgcgacgttc gtccagccat tgctctcggc gcgcatcatc acgagtttcc cccggcgtga
     1081 ccacttctcg cgtgtggagc cggacacgca aaaacgaatg ggcgtgccga aaaccgagcc
     1141 acagcatgct gcggttttcg tgcagcttca tgcgttcgcg gatgcgccaa ggatggctga
     1201 ttgaacagta ggtaccgacg ttctgatagc gaagataagt ggtgtggtag gcaccggcca
     1261 ggagaaaaaa gtctccacgc cacccgtccc ggacatgcac caacgtcacg tcctcggtgg
     1321 accaggcatc caggctgtcg aagtcttccg gtgcctcaac gctccattcc tccacgtagc
     1381 agatgacgtc tttggccgca accgccggtt tgagcccgag agcagcgaga cgctcaccga
     1441 ccgccagcac ctgggtaaag gtcatcggac cgcgagtctc ccagcggcgt tcacgcacgg
     1501 atcaaatctc cctcagacga ttaccggctc ggacgcacgg cgcgctgtga gatggtggta
     1561 tcagcaaggt aacgatcgag tgtatctttc acggtcgcgg tcatcaactt tttgacgtca
     1621 tcttccgaaa accagacgac agtatcggtc tgggctcctt cgaggctccg gctggtgctg
     1681 ctacgatcca cgttgttccg cgccgtgatc gtcagtttgt tgctggtcgt aagataggtg
     1741 gaaaacaatc ggctcttcgc attcgccgcg aattcgaaca attgcccgct gatcacgatg
     1801 tcggcatttg gcgcggatcc ggctggtgcg acacgcacat cccatgaccg atcgagccat
     1861 ccggatgccc tcaagcgatc ggcgagggcc tgggcataca cctctccggg cttttctccc
     1921 gtcacattaa aatatgtgac ccctccccac aggtgggtgc gcatcccgat gcggtgttta
     1981 tccgcccgcc ggtcttcaaa cggttcaatg acgattttga cggcttccgg ttccgcgacc
     2041 tgaaccggtt gctgctgcgg tagcacgtct aaatggagcg tctctccgct tcccccgcag
     2101 cccgacgtag tcatgagtcc gaccgccagc agaattcccg cgcccacccg cataagcgaa
     2161 tgtatcacga tgccctcctt aggaatggcg cgagcggggc ctcgacgccg catgaaccat
     2221 gtgcgttgtg atgtcgcaag gccggcagcg gcgatccggt tgcaagcaaa cgatcaccaa
     2281 atgcgcgcta gtctacagaa atccccgcgc tcgtacaatt acagcgacac aatcgggagc
     2341 atggcgcgga acgcttccag actcagacct gccaaatccc cgctccgcag ccgtgcatcg
     2401 tcgaagggga tgaaccggac gatcttcagg aatgtgcgtt cggccagcac cgcggcggtt
     2461 tgagccttcc gatcatgagt gatcggcagc gtgtaacctt cgcctctttt ccactcccaa
     2521 agggttggcg ccagtgacaa ttcaagatgc cgtcctgcaa tggtatggaa tcggtcgacg
     2581 aggcgcgctt tgtattgttt ggtcaagctc ccttcgagca gccaggcaca ggcgacatgg
     2641 tgtccccacc agaccagtgt acggaacgta cgcttctcag gaccatgaaa gtactttgga
     2701 caatccagat attggtaggg gaaattctcc aagtgctcgc ctttcacgaa ctgcgcggag
     2761 aagccatcgt gaggaagcgc aggaagctgg ctacggctca attccgcttt caatgcttca
     2821 cctgtcttct ccaactgaac ccggatcttc tcactgattt tggccttttt ctggaagaac
     2881 tgcgcgtcga tcagcagtgc agtttcctca tcggtaaaag acagcgcgtt cgtcatactg
     2941 agtacaccga gagaggagca aatgcttggg cgaacaggat cacaggagcg tcgggtacga
     3001 gtggctgcat attgaaccgt tcagcaatgt caaaagtttg gccagccgac tcgtcctgat
     3061 tggcgttcat cttggacgtg cgagagggaa acctgccgcc atcttatttt tggtgtgttt
     3121 cgaacgccgt caccgtcaag acataaagcc ggcggcattc cgcgcaacgg atcacggcaa
     3181 ggtcttcaaa gacatccatc tcgcgcaacg tgagggcggc cgcatgtgcc tccagacagg
     3241 cagcgaaccg gacctgcgac ggcaattgag cgtcggattc tccggtggac ggctccgtct
     3301 gttccgatgc ggaagtccga accgcgacat cgccgagcat aagataatga gcaaaccggc
     3361 aggatgtgca agccaggatg agctgattgc ccccggtgat ccgtctgacc gatacgcata
     3421 atggatgcac cgaccagcat tgttggagga aagccccgct gtggaagacc tgtcccatcg
     3481 cgtccaacgc ttaccaattc cagagcgcga acatgagcac catacctccc aagtagggaa
     3541 ttgcgcagga gtagaaaaga gccgcgcgcg cctgttcctt cgaaccggtc ctggcacact
     3601 gttctttgta gacgaattcg taggacaaaa cgagaagcgt gagacagagc gccaagatcc
     3661 gactcgttct gggccaggta tacagaccgc tggctagaaa gtttgcggtg aagaatccac
     3721 tgatgcttaa aatggccgga gccagcccat agcgaactac ctgcgcaaac atgttatgcc
     3781 aagcggggtg agcgtcgtgt ggacgtgaac tgcgctcgga agtcacgaac caacagcgac
     3841 gggagattcg gccttttcgc cagcggatgt cgaggagtgt gtccggcccg cgcggcatga
     3901 cttgcatact cgaggccgag ccttcagaaa tgagaatttc ccgcttgcaa gacagctgta
     3961 gtgcacgaat tcatcgcaga cagtgcagcg ggaccagccg ccacgaagga tggtcttgtc
     4021 gcgatacagt ccctgcttac agactttgca gtgtcgcggc tcgatgggga gcagcagaga
     4081 ttcccactca cctcccgctt tgcgaatgct catggactgg aagtatagcg ggggcatctg
     4141 cgcaaaaaca aggagaagaa cccctgcccg actgatgcca tggacgggag agcgcggtgt
     4201 cgagcagggc gggatacgcg agtccaacca tcgcaagagc aacagaacca acccactcgt
     4261 ttgactcccc ctggggcctt tgttactctc cgcgcgggtt tgtgtgcact tgtgtcaacg
     4321 tagcactctt atgaatatta aggactactt ggaacagaaa cgcatcgaag tggaccgctt
     4381 cttggatgaa gtgactccac cagccggcac gccgccggtg acccttcatg acagcatgcg
     4441 ctacagcctt ttgggcggcg gcaaacggat cagaccaata ctcacgattg cttcggcgga
     4501 ggccctgggt acgaatccac ctggcctgat ggcagcagcc tgttcattgg aactgatcca
     4561 cacttactcg ctgatccatg atgacttacc ctccatggat aacgatgact ttcgccgtgg
     4621 taaaccgacg aaccacaaag tctatggcga agccatggcc atcctcgccg gcgatgcgct
     4681 gctcacgatg gcctttgact tgatcagccg ccccgatctc atgaaaggtt gtgatcctgt
     4741 acgccaggcg cggatcattc aagaactggc cttcggctcg ggcaatatgg gaatggtcgg
     4801 gggacaggtc ctcgacatcc aagcggaaaa ccaggacatt gacttgccca ccctccagaa
     4861 cattcacaaa cacaagaccg gtatgctgat tcgcgccgcc gtccgaatgg gcgccattgc
     4921 aggcggcgcc aatgatcgac aactcgacga cctgacagga tatgcggaag gcatcggtct
     4981 ggccttccag atcgcggacg atgtgctgaa cgtgacaggc acgcgggaag agctcggcaa
     5041 gaatcccaac accgacgctg agcgcggcaa gaaaacctat ccgacattct acggagtgga
     5101 aggagcgaga caattcgcag acgcatgcgt cacgcgcgcg atcgaccggc tctcgtcctt
     5161 cggctctacc gctgatcctc tgcgagagat cgcccgatat atcacgagcc gccggaacta
     5221 gggaaagggc gcccatgaca gatggcacga ccttgggatg cggacacact gccagcactg
     5281 gccggctatc cgccatccgc tcttcccaat gaaagctctc gttaccggcg ccaccggctt
     5341 cgtcggtggc gcggtcgcac gtgcccttgt cggagcgggt gtggatgtcc gagtcctcat
     5401 ccgttccaac tccgatctcc aaaatctctc gggtctttcc gtcgagcggg tcgagggcga
     5461 tctgcgcgac ccggcctcac ttcggccggc gctggccggc tgtcatcatc tctatcacgt
     5521 cgccgcccac tatgcgctgt gggccaagga tccggcaatt ttctacgacg tcaacgtcac
     5581 cggcacccgt aatgtgctgt cagccgcaaa gctggccgga gttgagcgta ccgtctattg
     5641 cagcaccatc ggtgccattg gattaccgcc cggcggcgga ttgggctctg aagaaacacc
     5701 tgtctcgttg gaccaaatgg ccggtcatta caaacgatca aaatacctgg cagaacaaga
     5761 ggtgctcaaa ttcgctcacg aagggctccc ggtcgtgatt gtgaatccca gtgcaccggt
     5821 cggagctgga gacgtcagac ccactccgac aggacaagtg attgtcgatt tcatgaaagg
     5881 ccgcatgccc gcctacatcg aaaccggaat gaacattgtg gacgttgagg acgttgccgc
     5941 cggccacctg ctcgccatgg agaaaggacg tcagggtgaa cggtatatcc tgggatgcaa
     6001 gaacctgatg cttagagaag tatttgacat tctcagcggt ctcacgggcg tcaaagcgcc
     6061 ctcgctccgg cttccgcgtt ggaccgttct ccctcttgcc tatgcgaacc aatgggtagc
     6121 aaacgtgacc ggccacccgc ctcgcatccc gctggaaggc gtcaagatgg ccaaatacag
     6181 aatgcactac gattgtggca aggccatccg cgaactgggt attccccaga caccgccgga
     6241 agtggcgctg gagaaggcgg ttcgctggtt ccgctcccat ggatatgcgt aaaggatcag
     6301 tgtcggttac ccgtcttcag actgaccgta atgcactcga ctcccttttt gcaggtggca
     6361 atcaggatga tagctttgta tagtgaatgg actccgtatg aaaactaagt tgctcggtgc
     6421 agcagttctc atcgtcatag ccggtctagg cattttgagt tgggtcggtt atcaactctt
     6481 tactaccggg ttcagcgcga agacggaacc gcatgcgctg gagatctgga tggcgcggca
     6541 gattcgccat ctcgctatcc cgatcgacaa gcgaaacctt acaaatcccg tgcccctgac
     6601 ggcggaggtg cgacgggaag gacgcgctca tttcgcggat cattgtgctg tgtgtcatgc
     6661 caatgatggc agcgggcaga cccccatcgg caagaacgtc tatcctaagg cgccggattt
     6721 gcgactgcgt gagacgcagt cgatgtcaga cggcgagctt ttttgggtga ttcataatgg
     6781 catccgcttt acggcgatgc cagcctgggg cgccggcgaa ccggaaaagg acttggacag
     6841 ctggaagcta gtgcatttta tgcgccatct gccgcaactc actcaggaag aactggacga
     6901 aatgaaaacg ctcaatccga agacaccgaa cgagctcctc gaggaagcgg cgttcgaacg
     6961 gttccttgag ggcgccgacg ccggcggaaa aagcttagaa gggcatcatc actagtcata
     7021 gcttggagga aggagagcgg attcaccatg tttcactcat taattctcgc ggtgctttgc
     7081 gtgatgctcc cgacgacggc cctggcacac ggaacgggcc agcacgtatt gggaacagta
     7141 gtcgcgatcg actcgaaaca tctcgaggtt aaaacccaga aaggcacgac cgttgaggtg
     7201 ctgatcaaca aacaaacgcg gtttaaagaa aaaggcaatc caaaaggggc gaatctcccc
     7261 agtgtcggtg accgcgtggt agtcgaggcg atcaaggatg acaaggccct taccgccacg
     7321 gaggtccatt tttcgtcatt caagaagccg gcgcaagagc cagcgcccct gccccaacag
     7381 acgccggcgg ctgggacacc ggctccatag gacgtagtgc ggtgcgtatc gttcatcttt
     7441 cccagcagat gcaatccgct ccgttcagcg gaggattcca cggcgccatg gtgcgccttc
     7501 tcatcacagg cattgcggtg tttttggcgg tggccacggt gcccgggctg gaagcagata
     7561 gcttcggagc aggagtagcc gcagtgctgg tgctcacgtt gctgaatctg ctgcttcggc
     7621 cgctgttgtt cattttaacc ttgccgctca tcgtgctctc cctgggactg tttttggttg
     7681 tcctcaacgc gctgctgttg gagctcaccg catatatggt gaagggcttc acagtatcag
     7741 gcttctggcc ggcagttggc ggagcgctcg tcatcagcgt cgtcaccagc gtgctgaatc
     7801 ttttcacagg cgattaccgc caggtcgaca cccgtacgac gcagccgagg gagccaaaag
     7861 ttatcaaccc tccagagtag aaactttttt gtcagagtga tcaaaaagat ctccagcgaa
     7921 gccgcagcga gcgcggaggt gaggcgtgct atgttcggca ccttgaacct ctgcgcgata
     7981 agggaacaaa gcgtggtgaa aggcgcgtcc cggtgcaccg ggcgggcggg tgaagagacg
     8041 gttttcaaca cgctgctgcg gctgcttttc attgaattga cgttgatgca ttgctacaat
     8101 gcgcgcactt cttagctgaa cgcggaatat gacaccagcc attccacaga gtaagccgcc
     8161 gtctgagacc cagcttcaac ggacgctcaa ctcagtcctc aacgacatac cggttgattc
     8221 agcgcttgcc gctattttgc accgggagaa cggtccgctc gttggacatg ccacgcgagg
     8281 attcacggct cgggacgtcc aagcgatcct acgcacactt tccgctccgg cgcttattac
     8341 cgctggcgca caggatcagg acgggggccg cacgatgcgc ttgcgcatga ttaccccagg
     8401 agctaagtcg ctgttgggcg ttccgctacg atatcgaaac cgtacctacg gctttctcgt
     8461 cataggccga aaagagagcg cggcgtttac gaaaaaggaa cgcggtttga tggaccaggc
     8521 cagcgaagac gtcacaaaag ctttggaccg agaaaagctc ttcgacatga acgtggtcct
     8581 gagccgtccg ctggtgaacc aggaacaggt gtctgtggcc attccatccc cgcctctctt
     8641 gtccgcgccg gtttcgcagc tcacgccgca gcttcaggag aacatcacgg ctctcctgac
     8701 cgacgccgcc gagacggtca cttacgatcg gtcgtgggta tgccactacg atcccattgc
     8761 cggcagcgtg gaggttctcg gcatggcagg agacctgaaa ggcgagcaca aagacggcaa
     8821 gaaagacatg cggccgggtc atcgccttgc tctggacagt tctgcggccg ggtgggccat
     8881 tcgtcatcgt aaaccccggg tggaccacga tttggcgtcc acccaaggcc gcttcctcga
     8941 tcataaacag ctgttcaaag atcggttcca gtcatcattg gtatttccgt tttttgtcag
     9001 gggacaggtt ggcggtacgg tgacgttggc ttcgcgtgaa gcggaccgct accaaccgac
     9061 ggatgcgcgg acactggagc cgttgattct aaaacttgcc gacctgctcc aagcgccgtc
     9121 ggcagtagag gccgctcccg cgcctgttgt cgacgaaggc gggacctccg ctgctccggc
     9181 tcagcccgct gtgatctcgc aagaaccgat tattcgcaag caagagcgtc aggccgcgat
     9241 cggagaattc agcgcctttc tcgccacgga ggtacgcgaa ccgctcggct cgattcgagc
     9301 ccagttggaa gaggtcaccg gagaaggcgt gctggatttt gatccccaaa cccgtgtcga
     9361 aaacgccatg cgcgatctga tgagggtcga agcgattctc aatgagattt tggacttcgc
     9421 gaaaccgctg gaattgaatc gacgcctcgt ccgtatcccg gaggtcatcg aaagcgcgct
     9481 gaccgtggtg gccaatgacc tcgaaatcac gagaatccaa gtcgggaaag agtatgctcc
     9541 catccttgcg cctgtcaggg gcgacgaggc aaagctgcaa caggtgtttc tcagtatttt
     9601 caagaatgcc tgtgaagcga tgagcccagg tggacggctg accatccaag tggcacaaca
     9661 tcgggcgggg cgaggcatcg aagaccatat tctcatcaag aacgacggag cacctattcc
     9721 gccggaaatc gtagacaaag tttttgagcc gttttttacg acaaaaaaag ctggatccgg
     9781 gttgggcctc gcgaccgtga agaagatcgt ggaagaacat ggcggctcaa tcgccatcgg
     9841 aagcgcacct ggcgagggca ccacggtcac catccggctg cccggtgtga gccgtggtcc
     9901 ttcattccgt catcgggggc gcggccggcg ccccccgcat cggcgatagt gctgccatac
     9961 tgaagatttt gttccctccc tggccggttt tagaactcgt tccaactgtg cgcagcattt
    10021 cttatccacc cacccgctgg cacgtcccga catgtcaatt tc
//
LOCUS       DASTQY010000042         2804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_774797, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000042 DASTQY010000000
VERSION     DASTQY010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774797"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFR82_01360"
     CDS             complement(<1..306)
                     /locus_tag="VFR82_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500275.1"
                     /translation="MIRYLFSLVIGCAGLCLAAMTPAAFGQITVTNLQTPYSFVTSAA
                     GKDYFISSVNGEPDTADNNGFITKLDGSGTLVDLKFIQGGTDGVTLHAPKGMALVEQT
                     "
     gene            572..1432
                     /gene="folD"
                     /locus_tag="VFR82_01365"
     CDS             572..1432
                     /gene="folD"
                     /locus_tag="VFR82_01365"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEU4500276.1"
                     /translation="MAARLIDGKALAQQVREGLVGASAAVLAKTGMKPGLATILVGDD
                     PASHVYVKNKQKACELAGIHVDDHKLSAHTTQAELLALIEKKNADQKIHGILVQLPLP
                     KHIDSRVILEAVSPDKDADGFHPYNFGRLVEGHPVFEACTPKGVIKMIESTGVPIEGK
                     RAVVVGRSNIVGKPLALMLLQRHATVTICHSKTKDLPAVCREAELLLVAIGKAKFVTA
                     DMVREGAVVIDVGTNKLPDGKLCGDVDYESVSQKAAWISPVPGGVGPMTIAMLLDNTV
                     ESAKRMAGMK"
     gene            1675..2562
                     /locus_tag="VFR82_01370"
     CDS             1675..2562
                     /locus_tag="VFR82_01370"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEU4500277.1"
                     /translation="MSQVPKRLKEVLDQGQFAVTIEYNPPKGTNINGVLENAKQLLGR
                     VHGVNVTDNTAAVMRAGSLPVCRLLYELGHDPVMQLTCRDRNRIAMQSDLLGAHMLGI
                     RNILCLTGDYPTVGDHKDAKPVYDLDSVQVMGLVQGLNNGRDLAGNKLDGSTAFTIGA
                     AVTPEAELVGPVLAKFEVKVKAGAQFFQTQAIYNTDQFASFMNAVRPFKVKVLAGILL
                     LRNAKMAEFMNANIPGMSVPQAMIDELRAAGDKSEDVGVEIAVHSIQAVRPHCDGVHI
                     MAIKGTHRLGEIITKAQLT"
     gene            2559..>2804
                     /locus_tag="VFR82_01375"
     CDS             2559..>2804
                     /locus_tag="VFR82_01375"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEU4500278.1"
                     /translation="MMTVPDLQRCKRDKKKVIVVTAYDALFTRLLEQAGIEAILVGDS
                     LGVVVQGKPNTLAVTMEDMLYHTKLVAGTAQKSLVIGD"
BASE COUNT          646 a          733 c          848 g          577 t
ORIGIN      
        1 cgtctgctcc accagcgcca tcccctttgg cgcgtgcaag gtgacgccat ccgttccccc
       61 ctgaataaat ttgagatcta cgagagtccc gctgccatcg agtttggtaa taaagccatt
      121 gttgtcggcg gtgtccggtt cgccattcac actcgaaata aaatagtctt ttcccgcagc
      181 gctggtcaca aaactatagg gcgtttgcag gttggtgacg gtgatctgcc cgaatgcagc
      241 aggggtcatt gccgccaggc acaggcccgc acatccgatg acaagactaa acaggtaacg
      301 gatcataagg gttaggtggg gggacggtta tggtggcgtc gcgatgcaca tcggaatcat
      361 cgtagccgtg acgtggagaa gctgtcaaga aatcggcgga aaggccaact gccatgctgt
      421 gcaaaaccgc gtccgcccat acgttgactt gcataaaatt gccctgttaa ggtgccgttc
      481 tcttaagcaa gtcactggtg gaccaggctc atgaggagca tccgatccac cgcgcacctt
      541 ctgactccgg attatgcaat gggaggacgt tgtggcagct cgattgattg acgggaaagc
      601 attggcgcag caggtgcgcg agggtctcgt cggagcatct gcggcggtgt tggcaaagac
      661 ggggatgaag ccggggctcg cgacgattct cgttggagac gaccccgcgt cacacgtcta
      721 tgtaaagaac aagcagaagg cctgcgaact ggcaggcatt cacgtcgacg accacaaatt
      781 gagtgcccat accactcagg ctgaactcct ggcgctcatt gagaagaaaa acgccgatca
      841 gaagattcac ggcattctcg ttcagctgcc gttgcccaaa cacatcgata gccgcgtgat
      901 tttggaagcc gtttctcctg ataaggatgc ggatggattt catccgtata atttcggccg
      961 actcgtggaa ggccatccgg tgttcgaggc ctgtacgccg aagggcgtga tcaaaatgat
     1021 tgaatccacc ggcgtcccga tcgaaggcaa gcgtgcggtg gtcgtcgggc gcagcaacat
     1081 cgtgggcaag ccgctcgcgc tcatgctgtt acagcgtcac gcgaccgtca cgatctgcca
     1141 ttccaaaacc aaggacctcc ctgcagtatg ccgggaagcc gaactcctgc ttgtcgccat
     1201 cggcaaagcg aaatttgtca cggccgatat ggtccgcgaa ggcgcagtcg tcatcgacgt
     1261 cggaacgaat aaattgccgg atggcaagct ctgtggcgat gtcgattacg agtcggtcag
     1321 ccaaaaggca gcttggatta gtcccgttcc gggcggggtc ggacccatga cgattgcaat
     1381 gttgctggac aatacggtgg agtctgctaa gagaatggct gggatgaagt aggtcggatc
     1441 agggcacctt ctgcgctcgc gcactgcgca cgcagcaaaa aggggtttat cccgtcgcca
     1501 agatccattg gacgggctct gggtcgaaca agttctgttt ggcggtgctc gcatgggaaa
     1561 agggcgcgtc tcggcgcgcc cgaaggtggg cgggtgccat gatgcgcgcg gtggaaggtg
     1621 cccatgctcg gatctccttc atcgaaaaaa ggaagatgtt ggtgaggtaa ggaaatgagt
     1681 caggtgccga agagactgaa agaggtcctc gaccagggac agttcgctgt cacgatcgaa
     1741 tacaatcccc cgaaaggaac gaacatcaac ggtgtgctag agaacgccaa gcaactgttg
     1801 ggtcgcgtac atggcgtcaa cgtgaccgac aacaccgccg cagtcatgcg ggcggggtct
     1861 ttgcccgtgt gccggttgct gtatgagttg ggacatgatc cggtcatgca gttgacctgc
     1921 cgagatcgaa accggatcgc catgcagtcg gacctgttag gcgcccacat gctcggcatc
     1981 cgcaatatcc tctgtctcac cggcgactat cccacggtcg gcgatcacaa ggatgcgaag
     2041 ccggtgtatg accttgattc ggtccaggtg atggggcttg ttcaagggtt gaacaacggg
     2101 cgcgatctgg cgggaaacaa gcttgatgga tctacggctt tcaccatcgg tgctgcggtg
     2161 acaccggaag ccgagctggt aggtcctgtg ttagccaagt tcgaagtaaa agtgaaggcg
     2221 ggcgcgcaat ttttccaaac gcaagctatc tacaataccg atcagtttgc gagcttcatg
     2281 aacgccgttc gtccgttcaa ggtcaaagtg ctggccggaa ttcttttgtt gcgcaacgcc
     2341 aagatggcag aattcatgaa tgcgaacatt ccgggcatgt cagtaccgca ggcgatgatc
     2401 gacgaattgc gagcggcggg ggacaagtcc gaagacgtcg gtgtggagat tgccgttcac
     2461 agcattcagg ccgtccgtcc tcattgcgac ggcgtgcata tcatggcgat caagggaacg
     2521 catcggctcg gagaaattat taccaaggcc caactcacat gatgaccgtt cccgatcttc
     2581 agcgctgcaa gcgcgacaaa aagaaagtga tcgtggtgac ggcgtacgat gcgctcttta
     2641 cccggctgct cgagcaggca ggcattgagg ccattctagt cggtgactcc ttgggagtag
     2701 tcgtgcaagg caagcccaac acactcgcag tgacgatgga ggacatgctg tatcacacta
     2761 aacttgtcgc cgggaccgcc cagaagtcat tggttatcgg ggac
//
LOCUS       DASTQY010000043         3871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_373833, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000043 DASTQY010000000
VERSION     DASTQY010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373833"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFR82_01380"
     CDS             complement(<1..294)
                     /locus_tag="VFR82_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HEU4500279.1"
                     /translation="MDIRTLLKVMVDQEASDMYLTVDSPPSYRVHGSTQPANTPPFTN
                     EQLEALALALMRGQQRSEFEEKMEMNLALYYKELGRFRVNIFRQRGNVGLVFRH"
     gene            complement(325..1386)
                     /locus_tag="VFR82_01385"
     CDS             complement(325..1386)
                     /locus_tag="VFR82_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEU4500280.1"
                     /translation="MDISKLLTFSAKEGASDCHISAGEPPMIRLHGDLKKLDHPPLTS
                     EETHALVYDMMSDAQRKNFEEKRECDFSFELGDIARFRVNVFVQNRGLGAVFRNIPTE
                     IIPMEKLGLPPIVRQLCDKEKGLILVTGPTGSGKSTTLASMVDYLNNTFEGHIITIED
                     PIEFVHKTKKCLVNQRELGVHTLSFANALKSALREDPDIILVGEMRDLETIQLALTAA
                     ETGHLVFGTLHTSSAPKTIDRIIDAFPPAQQAQIRTQLSEALEAVITQTLLKKKAGGR
                     VAALEVMVATTAVRNLIREGKLHQIPGIMQASQKDGMQTMDMALVELATRGVVTKAEA
                     QSRSMSPNLFGGSPLSGAA"
     gene            complement(1501..2709)
                     /gene="bioF"
                     /locus_tag="VFR82_01390"
     CDS             complement(1501..2709)
                     /gene="bioF"
                     /locus_tag="VFR82_01390"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEU4500281.1"
                     /translation="MPACSRDSAVAEPSFMMFEDYLAQLASKHLTRRLLPLESGTGPL
                     VRVGGRDVLLFASNDYLGLAGHPDVVDAAVRATQTYGAGSGAARLVSGSLPPHQHLEE
                     ALARFKGTESALTLGSGYLANIGAIPALISSGGLILADRLCHASLIDGCRLSGADVRI
                     YRHNDMEHLARLLSKRRVGRRTLIVTDGVFSMDGDLARLGDLADLAEIHEADLYVDDA
                     HGTGVMGAHGRGTVEHFGLEKRIALHMGTMGKALGSSGAFLAGTHHAIQYLLHRCRSF
                     MFATAPPPASAAAALAALRIIEREPERRRRLWDNRERLFEGLRRLGFTMAATVSPIMP
                     ILIGRADTALRFAEHLLANDVFAPAVRPPTVPADTSRIRITVTSEHTPAQIDQALAAF
                     ERTGQHTGVL"
     gene            2806..3576
                     /locus_tag="VFR82_01395"
     CDS             2806..3576
                     /locus_tag="VFR82_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4500282.1"
                     /translation="MTYRIKIPAKTLQVDEAHLLSGLEHQLHRLQEYRRPLLVGFAVL
                     FLAVAAVGGVLWMDRQASQKAHELEREAARLLTARATADPKNTDTLLTQALAKYREVI
                     EQYPRTPTAPLAMYHLGNTQAQANDLPAAIETYQRFILLYGSQSALAGLVQQRLAYSY
                     LLKGDRDQAAKAFAAIVERPGALLKDQALFELARLEESQSRPEGALARYQELIKTYPT
                     SPFASEATIRTKVLDVKQTKDASPQPPPAVPASPGKKP"
     gene            complement(3632..>3871)
                     /locus_tag="VFR82_01400"
     CDS             complement(3632..>3871)
                     /locus_tag="VFR82_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU4500283.1"
                     /translation="PVGTKAKVEQLLDRVPTYKFPREAKPQFASAGSRWYKVRVGDTL
                     EKVSRRFRIPLKTLKTRNNLTGPTIRPGDFLVIGR"
BASE COUNT          784 a         1086 c         1178 g          823 t
ORIGIN      
        1 atgccggaac accagcccga cgttgccccg ttgccggaat atattcaccc ggaaccggcc
       61 cagctcctta taatagagtg ccagattcat ctccatcttt tcttcgaact cgctgcgttg
      121 ctggccacgc atcagcgcca atgccaacgc ctccaattgt tcattggtga acgggggtgt
      181 gttggccggc tgggttgaac cgtggacacg gtaagacggc ggtgagtcca ctgtcagata
      241 catgtcggag gcttcttgat cgaccatgac cttgagcagc gtgcggatat ccatcgtaac
      301 tcccttagta tcgtacgcga tgacttatgc ggctccgctc agaggcgaac cgccgaagag
      361 gttcgggctc atgctgcgtg actgcgcttc cgccttggtc acgacgccgc gtgtggccag
      421 ctcgaccagc gccatatcca tcgtctgcat accgtctttc tgactggcct gcatgatgcc
      481 cgggatctgg tggagttttc cttcccggat caagttccga accgctgtgg tggcgaccat
      541 gacttcgaga gcggcaacgc ggccaccggc tttctttttc agcaaagtct gggtgatgac
      601 ggcttccaag gcttccgaga gctgggttcg gatctgtgct tgctgagcgg gaggaaacgc
      661 gtcgatgatg cggtcgatag tctttggcgc gctagaggtg tggagcgtgc cgaagaccag
      721 gtgtcccgtc tcggcggccg tcagggccag ctggattgtc tccaagtcac gcatttcacc
      781 cacaaggatg atgtcgggat cttcacgaag cgcagacttg agcgcatttg cgaaggacag
      841 ggtatgcaca ccgagttcgc gctggtttac gaggcacttc ttcgttttat gaacgaactc
      901 gatcgggtcc tcgatggtga tgatgtggcc ctcaaacgta ttgttgagat aatccaccat
      961 cgaggcgagc gtcgtcgatt taccggagcc cgtcggaccg gtgacgagaa tgagtccctt
     1021 ttctttatcg cagagctgac ggacgatcgg gggcaagccg agcttttcca tcggaatgat
     1081 ttccgtgggg atgttccgga atacggcgcc gagtcctcgg ttttgcacga agacgttcac
     1141 gcggaatcga gcaatgtcgc ccagttcaaa agagaaatcg cattcccgct tttcttcgaa
     1201 gttctttctt tgggcgtcgc tcatcatgtc gtaaacgagc gcatgcgttt cttcagaggt
     1261 caacggggga tggtcgagtt ttttgaggtc tccatgaagg cggatcattg gcggttcgcc
     1321 ggcgctaatg tgacaatccg atgccccttc tttggctgaa aaggtcagca gctttgatat
     1381 atccatgaag acagctcctt agtaggacga tccgcgttac ttgagggcgg tgatcaatac
     1441 aggacgtgat gatgccacaa gttgatggga aagcaattaa atctcttgat tccgacgagg
     1501 tcagagcacg ccagtatgct gtccggttcg ttcgaatgcg gcgagcgcct gatcaatctg
     1561 tgccggtgtg tgttcagacg tcaccgtgat acgaattcgg ctggtgtcgg caggaacggt
     1621 aggggggcgg acggcaggag cgaacacatc gttggcgagc aggtgttcgg cgaaccggag
     1681 ggccgtatcc gctcgtccga tcaaaatcgg catgatcgga gagacagtgg ccgccatggt
     1741 gaagcctaat cgccgcagtc cttcaaacag acgttcacga ttgtcccaca ggcgacggcg
     1801 tcgttcaggc tcccgctcga tgatgcggag ggccgcgagc gcggccgccg ccgacgcggg
     1861 aggcggcgcc gtggcaaaca tgaatgaccg gcaacgatgc aagaggtact ggatcgcgtg
     1921 gtgggtgccc gccagaaagg cgccgcttga tcccaaggct ttgcccattg ttcccatgtg
     1981 cagggcaatg cgcttttcaa ggccaaagtg ctcgaccgtt ccacgtccgt gtgcccccat
     2041 gacgccggtg ccatgtgcat cgtccacgta gaggtcggct tcatggatct cggcgagatc
     2101 ggccaggtca ccgaggcgag caaggtcgcc gtccatgcta aagaccccgt ctgtcacgat
     2161 caacgttcgg cgcccgacgc gtctttttga gagcagcctg gccaggtgct ccatgtcgtt
     2221 gtgccggtaa attcgcacgt cggctccgct caaacgacag ccatcgatca agctggcatg
     2281 acagaggcgg tcggcaagga tcaatccgcc ggaactgatg agagcgggga tggcgccgat
     2341 gttcgcaaga tagcctgacc ccagcgtgag agcggattcc gtgcccttga accgggcaag
     2401 ggcttcctca aggtgttgat gcggaggcag cgatccggac actaatcggg ccgcgcccga
     2461 tcccgctccg taggtttgag tggccctgac ggcggcatcg acaacatcgg ggtgacctgc
     2521 aagtccgagg tagtcattcg acgcgaacaa taagacgtcg cgtcctccaa cgcgcacaag
     2581 aggtcccgtg cccgattcca gcggcagtaa ccgtcttgtc agatgcttcg aggccaactg
     2641 cgcgaggtag tcttcaaaca tcatgaatga cggttccgcg acggccgagt cacggctgca
     2701 ggcaggcatt gccggccatc atcgcattga caagttcgta acttccttag tataaggcgc
     2761 gaggataggt cgaggaaatg ggttcttgcg ttgtctgatg tcccgatgac ttaccgcatt
     2821 aagattcccg ccaagacgct gcaagtcgac gaagcgcacc tgctcagtgg attggagcat
     2881 cagctccacc gcctgcagga gtaccggcgt ccgttgctgg tcggcttcgc cgtgctgttt
     2941 ctcgccgtcg cggcggtggg tggtgtactc tggatggatc ggcaggcatc ccagaaagcc
     3001 catgaactgg agcgagaagc cgcgcgcctg ctgacggccc gtgcaacggc cgatcccaaa
     3061 aataccgaca cactgttgac gcaggccctg gcgaagtatc gtgaggtcat cgagcagtat
     3121 cctcgtaccc caacggcgcc gttggccatg tatcatcttg gcaatacaca ggcgcaggcc
     3181 aacgatctgc cggcggcgat cgaaacgtat caacgcttta tcctcctgta cggcagtcag
     3241 tctgctcttg cgggactcgt ccagcaacgc ctggcctata gttatctctt gaagggagat
     3301 cgcgaccaag cggccaaggc gtttgccgcc attgtggagc gcccgggagc cttgctcaag
     3361 gatcaggcgc tgtttgaatt ggcacgattg gaagaatcgc agtcgcgtcc cgaaggagcg
     3421 ctggcgcggt atcaggaact gatcaagacc tatcccacct caccgttcgc cagcgaagcg
     3481 acgatcagaa ccaaagtgct cgacgtgaag caaaccaagg atgcctctcc ccagccaccc
     3541 cctgcagttc cagcctctcc cggaaaaaag ccgtaaccgt gcgttcgaga cacaggccga
     3601 tgccgttcaa gagggcgcgc gctgggggtt tttagcggcc gatgacgaga aagtcgcccg
     3661 gtctgatcgt cgggcctgtg agattgtttc tggttttgag ggtcttcagc ggaatgcgaa
     3721 aacggcgcga gactttctcc aaggtgtccc ccacgcgcac tttgtaccag cgagagccgg
     3781 ccgaagcgaa ttgaggtttc gcctcccggg ggaacttata ggtgggaacg cgatcgagga
     3841 gttgttctac tttggccttc gttccgactg g
//
LOCUS       DASTQY010000044         8268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_372775, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000044 DASTQY010000000
VERSION     DASTQY010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8268
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372775"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="VFR82_01405"
     CDS             complement(<1..174)
                     /locus_tag="VFR82_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU4500284.1"
                     /translation="MNSAAKLRHLARRQQRVRQRVFGTDERPRLNVFRSSGHIYAQII
                     NDLKGTTLAAASSL"
     gene            complement(183..719)
                     /gene="rplF"
                     /locus_tag="VFR82_01410"
     CDS             complement(183..719)
                     /gene="rplF"
                     /locus_tag="VFR82_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU4500285.1"
                     /translation="MSRIGKKPIPLPTGVDVKVAGSVVSVKGPLGKLDWTLKPGIGVT
                     VDKGQVVVTRSTDERKVRAMHGLVRAELNNMVEGVTKGYERNMEITGVGYKAAIQGRT
                     MSFNVGYINPVLYPVPTGIDVKVDKQTLINIKGSDKRLVGQVAANLRAIKPPDVYKQK
                     GVRYVGEVLRKKEGKTGK"
     gene            complement(750..1145)
                     /gene="rpsH"
                     /locus_tag="VFR82_01415"
     CDS             complement(750..1145)
                     /gene="rpsH"
                     /locus_tag="VFR82_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU4500286.1"
                     /translation="MVTDPISDLLVRLRNGFRRHHDVVTVPASKLKREVLRVLQAEGY
                     IQGIDSGTEDGHPILKVQLRYVGEGQPMITGMQRISKPGRRVYVGTKDIPRVRNGIGM
                     AILSTSKGIMTDQASRKAGLGGEVLCSIW"
     gene            complement(1218..1403)
                     /locus_tag="VFR82_01420"
     CDS             complement(1218..1403)
                     /locus_tag="VFR82_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEU4500287.1"
                     /translation="MSRLALRNKTATKPKFATRGYHRCGLCGRVHGYLRRFKMCRICF
                     RLLSLKGEIPGVRKSSW"
     gene            complement(1421..2080)
                     /gene="rplE"
                     /locus_tag="VFR82_01425"
     CDS             complement(1421..2080)
                     /gene="rplE"
                     /locus_tag="VFR82_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU4500288.1"
                     /translation="MADAKDPKKSKTAKQSGRPAKKDSGKAPQVLDDGSEESKFQPRL
                     RDTYRDRVVPALMKEFGYKNVMQVPKLERIVLNVGMSEAIQNVKLLESAATELGTITG
                     QKAVVTRAKKAIAGFKLRQGMPIGTKVTLRNRRMYEFFDRLVTLALPRIRDFRGVSPK
                     AFDGRGNYTLGLKEQLIFPEIQYDEVGSIHGMDITIVTTARTNDEGKALLTQLGMPFR
                     T"
     gene            complement(2168..2488)
                     /gene="rplX"
                     /locus_tag="VFR82_01430"
     CDS             complement(2168..2488)
                     /gene="rplX"
                     /locus_tag="VFR82_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU4500289.1"
                     /translation="MQKAHIRKGDTVVVIAGRDRGKSGKVLSVDRSAGKVVVEKLNII
                     KRHTKPNQKVKQGGILEKEAPLAISNVMFLCPVTKKPTRLGIRVQPDGRRVRFSKKSN
                     ELIE"
     gene            complement(2516..2884)
                     /gene="rplN"
                     /locus_tag="VFR82_01435"
     CDS             complement(2516..2884)
                     /gene="rplN"
                     /locus_tag="VFR82_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU4500290.1"
                     /translation="MIQNYTYMDVADNSGAKQAMCFHVLGGSKRRYASLGDVIVVAVK
                     EAIPQATVKKGDVSRAVIVRTTKEVRREDGSYIKFDRNACVLINKEGEPIGTRIFGPV
                     ARELRWKKFMKIISLAPEVL"
     gene            complement(2920..3186)
                     /gene="rpsQ"
                     /locus_tag="VFR82_01440"
     CDS             complement(2920..3186)
                     /gene="rpsQ"
                     /locus_tag="VFR82_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU4500291.1"
                     /translation="MAETEVKRREWVGRVISNKMNKTVVVAVERQVVHPIYHKVLRRL
                     TKFKAHDEQNSCNIGDRVRMIETRPISKDKHWRVVEILEKGRVD"
     gene            complement(3207..3416)
                     /gene="rpmC"
                     /locus_tag="VFR82_01445"
     CDS             complement(3207..3416)
                     /gene="rpmC"
                     /locus_tag="VFR82_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU4500292.1"
                     /translation="MDLKELRQLSSDELAEKAKQLAQELFNLRFQLGTGRLENPMQIR
                     KTKRSIAQVKTVLREVEKTDHKKAS"
     gene            complement(3441..3854)
                     /gene="rplP"
                     /locus_tag="VFR82_01450"
     CDS             complement(3441..3854)
                     /gene="rplP"
                     /locus_tag="VFR82_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU4500293.1"
                     /translation="MLAPKKVKFRKMMKGRMRGKAYRGGHITLGEFGLKALEPGWVTS
                     RQIEAARIAITRYVKRGGQVWTRIFPDKPITKKPAETRMGKGKGNPEYWVAVVKPGRI
                     LYEMGGVTPDIAYEAFRLASHKLPIATKCVTRGEF"
     gene            complement(3869..4540)
                     /gene="rpsC"
                     /locus_tag="VFR82_01455"
     CDS             complement(3869..4540)
                     /gene="rpsC"
                     /locus_tag="VFR82_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU4500294.1"
                     /translation="MGQKTHPIGYRLGYNYTWSSRWYASKDYAKLLHQDIKIRKVVKQ
                     KLFHAGVAKVEIERSGDQTRVIIHTARPGIIIGRKGAEVDKLKAELEKQYGGQVYITV
                     KEIKKPELDAQLVSENVATQLEKRVAFRRAMKRSVQSALRLGAQGIKIMIAGRLGGAE
                     IARTEWYREGRVPLHTLRAEVDYGFAEAHTTMGQIGVKTWIYKGEILPAQPLKPESAF
                     ERRLG"
     gene            complement(4592..4951)
                     /gene="rplV"
                     /locus_tag="VFR82_01460"
     CDS             complement(4592..4951)
                     /gene="rplV"
                     /locus_tag="VFR82_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU4500295.1"
                     /translation="MAEARAVLRFVRVAPRKARPVVDMIRGQQVPMALAMLKHLPRHA
                     ARVIEKVLRSAVANAEQKELGDSDSMTVSKAFVNCGPIYKRFRARSMGRANAIQKRTS
                     HITVVVEARGMQEKSRS"
     gene            complement(5001..5288)
                     /gene="rpsS"
                     /locus_tag="VFR82_01465"
     CDS             complement(5001..5288)
                     /gene="rpsS"
                     /locus_tag="VFR82_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU4500296.1"
                     /translation="MPRSISKGAFVDDHLLKKVEQMNQTKDRKIIKTWSRRSTVIPDM
                     IGHTFAVHNGKKFIPVFVTENMVGHKLGEFAPTRFFKGHGQAKTEKAVALK"
     gene            complement(5281..6123)
                     /gene="rplB"
                     /locus_tag="VFR82_01470"
     CDS             complement(5281..6123)
                     /gene="rplB"
                     /locus_tag="VFR82_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU4500297.1"
                     /translation="MGLKAYKPTTSGRRGMTIVTTEELTKKRPEKSLTSFLLRTGGRN
                     NDGRTTVRFRGAGHRRLYRQIDFRRDKVGISAKVSALEYDPNRSARIALLTYRDGEKR
                     YILAPIGLQVGDVLQSGVDAEVRTGNALPLANMPLGTTIHNIELKPGKGGQLIRSAGG
                     FAQVMGRDGNYVQVRLKSGEMRRVLATCMATVGQVGNLDHENINVGKAGRSRWLGRRP
                     HVRGVVMNPVDHPHGGGEGKSGQGNPHPVSPWGLPTKGYKTRQNKKTDKFIIARRKSG
                     VRNA"
     gene            complement(6158..6451)
                     /locus_tag="VFR82_01475"
     CDS             complement(6158..6451)
                     /locus_tag="VFR82_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU4500298.1"
                     /translation="MKLNLHSVLIQPLLTEKVTALRESSNTVGFLVHPEANRLQIKQA
                     VETLLKVKVERVNVLNVQGKIKRLGRFSGKRSDWKKAFVKLKEGEKLELYESA"
     gene            complement(6448..7071)
                     /gene="rplD"
                     /locus_tag="VFR82_01480"
     CDS             complement(6448..7071)
                     /gene="rplD"
                     /locus_tag="VFR82_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU4500299.1"
                     /translation="MPTVDVLDLNKRKVGSAELPPEVFGCKPHATLVHEVVVMQRACE
                     RQGTASTLRRGEVRGSGKKPWKQKHTGRARAGSVRSPVWRHGGSVFGPKPRSYAFSMP
                     KKKYRTALQSALSSKVTDGQLFVVSSLSLEQPKTKALAKALQLFAAGTQVLVIAGEGQ
                     DGLLQAARNLSKVQVVTPDGLNVYDIVRAGTIVIPERALARVKEVWA"
     gene            complement(7074..7694)
                     /gene="rplC"
                     /locus_tag="VFR82_01485"
     CDS             complement(7074..7694)
                     /gene="rplC"
                     /locus_tag="VFR82_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU4500300.1"
                     /translation="MTNGLLGKKLGMTQMLDETRLTPVTVIEAGPCRVVMVKTKERDG
                     YEAVQLAFGQVKDRKLSKAQLGHLKKHEATASRILREFKKDGDVAVGQTVKADIFKKG
                     DWVDVIGVSKGKGFQGVVRRHHYSGGPESHGSMFHRAPGSIGASSFPSRVWKGKTLPG
                     HMGAERVTSQRLKVVETRPDENLLFVRGAVPGAANGLVVVRKSKKG"
     gene            complement(7759..8073)
                     /gene="rpsJ"
                     /locus_tag="VFR82_01490"
     CDS             complement(7759..8073)
                     /gene="rpsJ"
                     /locus_tag="VFR82_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU4500301.1"
                     /translation="MVKVDQRIRIRLRGFDYRVLDQSVAEIVETVRRSGAKIVGPIPL
                     PTRIEKFTVQRATHADKKAREQFEIRTHKRLMDIMEPTPETMDSLMKLNLAAGVDVEI
                     KL"
     gene            complement(8097..>8268)
                     /gene="tuf"
                     /locus_tag="VFR82_01495"
     CDS             complement(8097..>8268)
                     /gene="tuf"
                     /locus_tag="VFR82_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031793.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU4500302.1"
                     /translation="TGVVSLNPGVEMVMPGDNVSVTAELISPIAMDQGLRFAVREGGK
                     TVGSGVVTEILA"
BASE COUNT         1516 a         2625 c         2060 g         2067 t
ORIGIN      
        1 cagcgaggag gcggcagcca gcgtcgtgcc cttcaggtcg ttgatgattt gtgcatagat
       61 gtgcccgctg ctgcgaaaca cattgagccg gggacgctcg tcggttccga agacccgctg
      121 acgaacccgc tgctggcgac gtgcaagatg acgtaattta gctgcgctgt tcatggttca
      181 acctattttc ccgtcttgcc ttctttcttt ctcaacactt cgcctacgta acggactccc
      241 ttttgcttgt agacatcagg tggcttgatc gctcgcagat tggccgcgac ttgccccacc
      301 agccgcttgt ccgaaccttt gatattgatc agcgtttgct tgtcgacctt gacgtcgatg
      361 ccggtcggca ccggatacaa caccggattg atgtatccca cattgaagct catggtccgt
      421 ccctgaatgg ccgccttgta accgacgccg gttatttcca tattgcgttc atagcctttg
      481 gtcacaccct cgaccatatt attcaactcc gcgcgaacca ggccgtgcat ggcgcgtact
      541 tttcgctcgt cagtggaccg cgtgacgacc acctgtccct tgtcgacggt gacaccgatt
      601 cccggcttta acgtccagtc gagcttccca agcggcccct tcaccgacac cacggatccc
      661 gctaccttca catcgacgcc ggtcggaaga gggatcggtt tttttccaat gcgtgacatg
      721 ccttcctcat ctttcctgct caataaccct taccaaattg aacacagcac ttcgccgccg
      781 aggccggctt ttcgcgaagc ttgatcggtc atgatgccct tcgacgtgga aagaatcgcc
      841 atgccgattc cgtttcgcac gcgggggatg tccttcgttc ccacatacac acgacgtccc
      901 ggtttgctga tgcgctgcat gccggtgatc atgggctgcc cttctccgac gtagcgcaat
      961 tggactttca gaataggatg cccgtcttcc gtcccagagt cgatgccctg aatgtaaccc
     1021 tcggcttgca gcacgcgaag cacctcccgc ttcaattttg aagcgggaac tgtcacgacg
     1081 tcatgatggc gccggaagcc gttgcgcagt ctcaccaata aatcgctgat aggatcggtc
     1141 accatgcgaa tccctctact ccctcttcat tcaccttcat tgaacaacgc ttcccgcttg
     1201 agtgtcgaat ctgattgtca ccaactggat ttccgaactc ccggaatctc gcccttcaga
     1261 ctgaggagcc gaaagcaaat ccggcacatt ttgaaccgcc gcagataccc gtggacacgc
     1321 ccgcacaacc cgcaccggtg gtagccacgt gtcgcgaatt tcggtttggt cgcagtctta
     1381 tttctgagcg ctaatctcga cacgctgact cctcccttgg ttacgtccgg aacggcatgc
     1441 ccagctgggt gagcagtgct ttaccttcat cgttcgtccg agccgtggtc acgatcgtaa
     1501 tgtccatgcc gtgaatcgaa ccgacctcgt cgtattggat ctccgggaaa atgagctgct
     1561 ccttcagacc caacgtgtaa ttaccccggc catcgaaggc tttcggagac acgcccctaa
     1621 aatcgcggat gcgcggcagg gccagtgtca cgagccggtc gaaaaactcg tacatgcgcc
     1681 ggttccgcaa cgtcaccttc gtcccgatcg gcattccctg ccgcagcttg aatccggcga
     1741 tcgctttctt cgcgcgggtc accacggctt tttgtcccgt gatcgtgccc aattccgtcg
     1801 cggcgctttc cagcagcttc acattttgga tggcttcgct catgcccacg ttgagcacga
     1861 tgcgttcgag cttgggcacc tgcatgacat tcttatagcc aaactccttc atcaatgccg
     1921 gcacaacccg gtctcgatag gtgtcgcgca accgcggctg gaacttcgat tcctcgctgc
     1981 cgtcgtccag cacctgggga gccttgccgc tgtccttttt cgccggccgg cccgactgct
     2041 ttgcggtctt gctttttttc ggatcctttg cgtccgccat acaaccttct tttccggcag
     2101 cctggtgaaa agtcctccag cgttgttctc gctgaaaggc ctttttgacc agctgctatc
     2161 tcccttacta ctcgattaat tcgttggact ttttactgaa ccgcactcgc cgtccgtccg
     2221 gctgcacgcg aatgcccaag cgcgtaggct ttttcgtcac cgggcagaga aacatcacgt
     2281 tggaaatggc cagcggggct tctttttcca gaatgccgcc ctgcttcacc ttttgattcg
     2341 gtttggtatg ccgcttgatg atgttcagct tttctacgac gaccttccct gcggaacgat
     2401 cgacggaaag gaccttgccg gactttccac gatcccggcc ggcgatgacc accaccgtat
     2461 cccctttacg aatatgagcc ttctgcagcg cgtgcacggt cacccctacg gttggttaaa
     2521 gcacttccgg agccagcgag ataattttca tgaacttttt ccagcgaagc tcacgcgcga
     2581 ccggtccgaa tatgcgggtc ccgatcggct caccctcttt gttaatgagt acacaggcat
     2641 tccgatcgaa tttgatatag gaaccgtctt cgcgccggac ttctttggtc gtccgcacga
     2701 tcactgcccg gctgacgtcg ccctttttca ccgtcgcctg tggaatggct tccttgacgg
     2761 ccacaacaat gacatctccc agtgaggcat agcgacgctt cgacccgccg agtacgtgaa
     2821 agcacatggc ctgcttcgcg ccggaattgt cggccacgtc catgtatgtg taattctgaa
     2881 tcatgatgcg tttctacctc gccgagcacg cccccgttac tagtcgacac gccccttttc
     2941 caaaatttcg acgacccgcc agtgcttgtc tttgctgatg ggtctggtct cgatcatccg
     3001 gacacggtct ccgatgttgc aagagttctg ttcgtcatgg gccttgaact tcgtcaaacg
     3061 ccggagcacc ttgtgataga tgggatggac gacttgacgc tcgaccgcca caacgacggt
     3121 cttgttcatc ttgttgctaa tgacgcgtcc cacccattct cgccgcttga cttcagtttc
     3181 cgccatgctc tgcaaccctt tcgccgttag gacgccttct tgtgatcggt cttttcaacc
     3241 tcgcgcagca ccgtttttac ttgtgcgatc gaccgcttgg tctttcgaat ctgcatggga
     3301 ttctcgagcc gaccggtgcc cagttggaac cggagattga acaattcctg ggccagttgc
     3361 ttggccttct cggcaagctc gtcgctgctc aactgccgta actctttcag atccattgtg
     3421 ctcatcgttt cacgtccaaa ttaaaactca ccccgggtca cacacttggt ggcgatcgga
     3481 agcttgtgcg aagcgaggcg gaacgcctca tacgcgatat ctggagtcac accacccatc
     3541 tcgtaaagga tccggccggg cttgacgacg gctacccaat attccgggtt gcccttgcct
     3601 tttcccatgc gtgtttcggc cggttttttc gtgatcggct tatccgggaa aattctcgtc
     3661 cacacctggc cgccgcgttt cacgtaacgc gtgatggcaa ttcgcgcggc ctcgatctgg
     3721 cggctggtca cccatcccgg ctccaatgct ttcaacccga actcgccgag cgtgatgtgg
     3781 ccgccacggt aggccttgcc gcgcatccgc cctttcatca tttttctgaa cttgactttc
     3841 ttcggtgcta gcacagtgcc ctcgtttcct acccaagccg ccgttcaaat gccgactccg
     3901 gcttgagcgg ttgagccgga aggatttcac ccttgtagat ccaggtcttg actccaatct
     3961 gtcccatcgt ggtgtgtgct tccgcgaagc catagtcgac ttccgcgcgg agcgtgtgca
     4021 acggcactcg gccctcgcga taccattctg tgcgcgcgat ttcagcacca ccgaggcggc
     4081 cggcgatcat aatcttgatg ccttgtgcac caagacggag tgcggattgg acgctgcgct
     4141 tcatcgctcg acggaaggcc actcgttttt ccaactgcgt tgccacgttc tcactgacca
     4201 gctgcgcgtc gagctccggc ttcttgattt ctttcaccgt gatgtagacc tgtccgccgt
     4261 actgcttttc cagttccgct ttcagtttgt ccacctcggc gcctttgcgt ccgatgataa
     4321 ttcccggtct tgccgtgtgg ataatgacgc gggtctgatc gccggagcgc tcaatctcca
     4381 ctttggccac acccgcatga aacagcttct gcttcaccac tttgcggatc ttgatatcct
     4441 gatggagcag ttttgcgtag tccttgctcg cgtaccagcg ggagctccat gtatagttgt
     4501 agccgagccg ataaccgatt ggatgtgttt tctgccccat gcgatccgca cctcagtttt
     4561 gcggcggcga gtctgccgat ccgctcaatg ttcaggacct tgacttctcc tgcatgcccc
     4621 gcgcttcaac caccacggtg atgtggctcg ttcgcttctg aatggcattc gcccgtccca
     4681 ttgatctggc acggaagcgc ttgtagatcg gcccgcagtt cacgaatgct ttcgacaccg
     4741 tcatcgaatc actgtcaccc aattccttct gttctgcatt ggctaccgcg gaacgcagta
     4801 ctttctcgat gacacgtgct gcgtggcgcg gaagatgctt caacatggcc aaagccatgg
     4861 gcacctgctg gccacgaatc atatccacca ccggacgggc cttgcgtggt gcaactcgaa
     4921 caaatctaag cacggctcgc gcttctgcca tctttctcat cctgtcatgc gtctcctccg
     4981 acgcacgaca actgatctcc ctatttcaat gccacggctt tttcggtctt ggcctgcccg
     5041 tgccccttga agaatctcgt cggcgcaaat tcgcccaact tatggccgac catgttctca
     5101 gtcacaaaga ccggaataaa tttcttcccg ttgtgtacgg caaacgtatg gccaatcatg
     5161 tcgggaatga cggtggatcg ccgcgaccag gtcttgatga ttttgcggtc tttagtttga
     5221 ttcatctgct cgaccttctt gagcaggtga tcgtcgacaa atgcgccctt gctgatcgat
     5281 ctaggcattg cgcacccctg acttgcggcg agcgatgatg aacttgtccg ttttcttgtt
     5341 ctgtctcgtc ttgtagcctt tcgtcgggag accccaggga gagacaggat gcggattgcc
     5401 ctgacccgac ttgccttcac cgcctccgtg tggatggtcg accgggttca tgacgacccc
     5461 gcgaacatgg ggacggcgcc cgagccatcg gctccgcccc gcctttccca cattgatgtt
     5521 ctcatggtcg aggtttccta cctggccaac cgtggccatg caggttgcca agacgcgtcg
     5581 catctctccg gacttgagcc gcacctgcac ataatttccg tcacggccca ttacctgagc
     5641 aaatcctccg gcgctgcgga tcaactgacc gcctttgccg ggcttcaact cgatattgtg
     5701 aatcgtggtg cccaacggca tattggcaag aggcagcgca ttcccagtcc tgacttccgc
     5761 atccacgccc gattgcagca catccccgac ttgcaggccg atcggtgcga gaatgtagcg
     5821 tttctctccg tctcgatacg tgagaagcgc gattctcgcc gagcggttcg gatcatactc
     5881 gagcgccgaa accttggcag agattcccac cttgtcacgc cggaaatcga tttgtcggta
     5941 caagcgccga tgaccggccc cacgaaaacg gaccgtcgta cgcccatcat tatttctgcc
     6001 gccggtccgg aggagaaatg acgtcaacga cttctcgggc cgctttttcg tgagctcttc
     6061 cgtcgtcacg atcgtcatgc ctcgccggcc tgacgtcgtt ggtttatagg ctttcagtcc
     6121 catgcaatac ctggctcttt ctactcgtga gcgctgttta tgcgctttcg tacaattcga
     6181 gcttctcgcc ttccttcaac ttcacgaagg ccttcttcca atcagagcgc ttgcctgaga
     6241 accgccccag gcgcttgatc ttgccttgca cgttcagcac gttgacgcgc tctaccttca
     6301 ccttcaagag cgtctcaacc gcttgcttga tctgcagacg attggcctcg gggtgaacga
     6361 gaaaacccac cgtattgctg ctttcgcgta gtgcggtcac tttctccgtc agcaacggtt
     6421 gaatgagcac gctgtggaga ttgagcttca tgcccagacc tccttgactc gagccaaggc
     6481 ccgttccgga atcacgatcg tgcctgcacg gacgatgtca tagacattca gcccgtcagg
     6541 tgtcacgacc tgaaccttcg acagatttct ggccgcctga agcaagccat cctgcccttc
     6601 accggcaata accagaacct gggtgccggc ggcgaagagc tgcagcgctt tagccagcgc
     6661 cttggtcttg ggctgctcga gcgagaggct ggacacgaca aacagctgcc cgtccgtgac
     6721 cttcgacgac aatgcacttt gcagtgccgt gcgatatttc tttttcggca tgctgaacgc
     6781 gtaacttctc ggcttgggcc cgaacacgga gccgccatgg cgccatacgg gcgatcgcac
     6841 cgacccggca cgggctcgac cggtgtgctt ctgcttccag ggcttcttgc cggatcccct
     6901 gacttcgccc cgccggagcg ttgatgcggt gccctgacgt tcgcatgccc gctgcatgac
     6961 caccacttcg tggacgagcg ttgcgtgcgg tttacaacca aagacttcgg gcggcaattc
     7021 tgctgacccg accttccgtt tgtttaaatc aagcacgtct accgtgggca tgcttatccc
     7081 tttttcgact ttctcacgac cacgagtccg ttcgctgctc cgggaaccgc cccgcggaca
     7141 aacaacagat tctcgtcagg cctggtttca acgactttca atcgttggga ggttacgcgt
     7201 tcagctccca tatgtccggg cagggttttt cccttccaga cccgtgatgg aaacgagctt
     7261 gccccgatcg accctggcgc ccgatgaaac atcgatccgt gcgattccgg tccaccggaa
     7321 tagtgatgcc gccgcaccac cccctgaaat cccttacctt tcgaaacccc gatgacgtcc
     7381 acccagtcgc cttttttaaa aatgtcggct tttacggttt gcccgacggc tacgtccccg
     7441 tctttcttga attctcttaa tatccggctg gcagtcgcct cgtgcttttt caaatggcct
     7501 aactgcgcct tggagagctt gcgatccttc acctgtccga aggccagttg aactgcctcg
     7561 tagccgtccc gttccttcgt tttgaccatg acaacgcggc acggtcccgc ttcgatgacg
     7621 gtcaccggcg tcaaccgagt ctcatcgagc atctgcgtca tgccgagttt tttaccaagc
     7681 aacccgttag tcataaatga tccgcttcgc agtggtgagt gctgcactca gtgtgttctg
     7741 ctcccgagct gggcccactc atagtttgat ttcaacatcg acgccggcag cgagattcag
     7801 cttcatgagg gagtccatgg tttccggagt tggctccatg atatccatca agcgcttgtg
     7861 cgttcgaatt tcaaattgct cacgcgcctt cttatccgca tgcgtcgccc gctgaaccgt
     7921 aaatttctcg atgcgtgtcg gcagcggaat cggaccgacg attttcgcac cgctacggcg
     7981 aaccgtttcg acgatttccg caacggattg atccaacacg cgataatcga acccgcgcaa
     8041 cctaattctg attcgctgat cgaccttgac catgcaatcc tctgttcgtc agtttcctag
     8101 gccagaattt ccgtgacgac gccggagccg accgtcttgc cgccctcacg cacggcaaag
     8161 cgcaggccct ggtccatcgc gatcgggctg atcaattcag ccgtcacgct cacgttgtcg
     8221 cccggcatca ccatctccac tcccggattc aacgacacca cgcccgtc
//
LOCUS       DASTQY010000045         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_572539, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000045 DASTQY010000000
VERSION     DASTQY010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572539"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFR82_01500"
     CDS             <1..309
                     /locus_tag="VFR82_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500303.1"
                     /translation="EQFFRQLSQVLVVGGRAVVAAVHPDIQRITGSDIEVQSKEKRFV
                     QIQGHIHEVRHLLAAAHDAGLTVAVMDEPAVTSTMVEYRPDWKKKLGCRALLLLTLVK
                     "
     gene            513..932
                     /locus_tag="VFR82_01505"
     CDS             513..932
                     /locus_tag="VFR82_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4500304.1"
                     /translation="MEGVPAGGFRTLGEIDGTNGLRFQPKESALAVALELLSSHTSGA
                     PIVNDAGNFVGFVSEFDLLGAIESGKDLSQITADKLMNKDHFVADESTTIADAIELMR
                     EKHLLNIPVVKQGQVAYSVTRHDLLRARIGLGPGIET"
     gene            complement(1450..>2047)
                     /locus_tag="VFR82_01510"
     CDS             complement(1450..>2047)
                     /locus_tag="VFR82_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU4500305.1"
                     /translation="ESRIALRLYSPEGETMERSLMEQSAATDEQIRNAIDQGPITKQY
                     FAQPESVVSSLNRLRSTEITSYLQYKQHGYMAVSLLSPGLKAEFEAHAEQELAHADRL
                     ATRIQQLGGVPIFDLQELAGKAAAIGIHPEQGTTLTEMVIENLLLERRQVEAYTALIR
                     ELGDKDLITREILLDILAETETHASELSDFLKRTSDKR"
BASE COUNT          458 a          519 c          582 g          488 t
ORIGIN      
        1 gagcagttct ttaggcagct ctcgcaagtg ctggtagttg gtgggcgtgc cgtcgtagcc
       61 gccgtgcatc ccgacataca gcgcatcaca ggctcggaca tcgaggtaca gagtaaggag
      121 aagagatttg tgcagatcca agggcacatt cacgaggtcc ggcacctact cgcagccgcc
      181 cacgatgcgg gactgacagt tgccgtcatg gatgaacctg ctgtcacatc caccatggtg
      241 gaatatcgtc ctgattggaa gaagaagctc ggttgccgcg cgctgctgct tctaactctc
      301 gtaaagtgac atgcatgact atgacagcgg atcggatacc gttgtcagta agcgcttgca
      361 attatggttg taaaaccccg ttgccggtga ttgccaatgt tgggagaacc gtgactcggg
      421 atggtgctag ttcctccacc ggctagaggc ctgataaagg aaaatgatct cttacaagca
      481 tgcttacaag catgaaggag gcctctatga ccatggaagg tgtgccggcc ggcggctttc
      541 gtacgctggg agaaatcgat ggcacgaatg gcttgcgttt tcaacccaag gagagtgcct
      601 tagcagtggc gctggagctt ctgtcttctc atacctcggg ggcacccatc gtcaacgatg
      661 caggtaactt tgtcggcttt gtaagcgaat tcgatttact gggagcgatt gagtctggaa
      721 aagacctgag tcaaattacg gccgacaagc tgatgaacaa agatcacttt gttgccgacg
      781 aatccacgac cattgccgat gcgattgagc tgatgaggga aaagcatttg ctcaacattc
      841 cggtcgtcaa gcaagggcag gtcgcctact cggtcactcg tcatgatctt ttgagagctc
      901 ggatcggtct agggcccgga attgaaacat agctcagcgg cctttgacgc gtcattcagg
      961 gtcaccgccc acaacttcaa actctccgga gggctcgttt cccacgccaa ggaaacgcct
     1021 tgttgctgat gcatgggccg ggcagcagag tgggagtatg ctcggcctat cggacgctct
     1081 gagtagaaac cgtatcctcg caggacttcc tgccagggag cgaaaaccta tcgaaacgcg
     1141 ctttcggtga gggttgttca cctacggcga cgttattgac cgaacgggtg aagcaatcgt
     1201 ttcacagtgt tttcccgttg atgcggccat cagtgatgaa aaaagattca gcgcatactc
     1261 tggatgtcgt attaatcggc ccggaaggct gcagcggggc atccgtgaca cagggcagcg
     1321 acctgtcgcc cgatttcctt gcggtgcaag tgggaatcgg gaacgagacg aggtccggaa
     1381 catcctccat gacatttgca cgacaccgca tctttaaaat tagcgcattt gagaaacgct
     1441 gatcagtcgt caccgcttgt cgctggtacg cttgagaaag tctgacagtt cgctcgcgtg
     1501 agtttccgtt tctgccaaaa tgtcgagaag gatttcccgc gtgatcaaat ctttgtcgcc
     1561 gagttcgcga atcaaggcag tataggcctc gacttgacgg cgttcgagta acaggttttc
     1621 gataaccatt tcagtgagcg tcgtgccttg ttcgggatgg atcccaattg ccgcagcctt
     1681 tcccgccaac tcctggagat caaaaattgg gacaccgccc agttgttgga tgcgtgttgc
     1741 caacctgtcg gcatgagcca gttcctgttc ggcatgagcc tcgaactcgg ctttgagtcc
     1801 tggcgaaagc agtgacacgg ccatatagcc atgttgtttg tattggagat agctcgtaat
     1861 ctctgtcgaa cgtaaccggt tcagcgaact gacaacggac tccggctgcg caaagtattg
     1921 tttggtgata ggcccctggt cgatggcgtt acgtatttgc tcgtcggtgg ctgcggattg
     1981 ctccatgagt gatcgttcca tggtttcccc ctctggtgag taaagacgca gagctatgcg
     2041 gctttcc
//
LOCUS       DASTQY010000046         3350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_572437, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000046 DASTQY010000000
VERSION     DASTQY010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572437"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..568
                     /locus_tag="VFR82_01515"
     CDS             20..568
                     /locus_tag="VFR82_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500306.1"
                     /translation="MLGTIPATETPPPCFHLTRALLASTLASCIFLLHPVALSAKSKP
                     TRAPRPEPELKVLELKISPNPYTVSAGSLEFSTVVQLPRELDGATILEVSSLVTSPSK
                     RSIRFLSLRKPLESPAITPISTDRQTIPVVLTWDGMDHTKAPAEAGIYQFELRAKLLS
                     NGEKGPRTLMLSWPKRGTLEVK"
     gene            complement(604..1356)
                     /gene="larB"
                     /locus_tag="VFR82_01520"
     CDS             complement(604..1356)
                     /gene="larB"
                     /locus_tag="VFR82_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEU4500307.1"
                     /translation="MNRERLERLLREVKSGKASIASALEQLRSLPFEDLGFASLDHHR
                     SIRQGFPEVILCEGKTPAQIRGIAGSLLKHHRPFLATRATRDIAAVIKRIDRRAVYHE
                     SARIVAVRDAQRRLHGRVLVITAGTSDVPVAEEARITAEVMGSRVETLYDVGVAGIHR
                     LLGRHQQLTDAHVIVVVAGMDGVLPSVVGGLVACPVVAVPTSRGYGASFNGLAALLTM
                     LNSCAAGVGVMNIDNGFGAGCLAHRINAFGIK"
     gene            complement(1353..2372)
                     /locus_tag="VFR82_01525"
     CDS             complement(1353..2372)
                     /locus_tag="VFR82_01525"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU4500308.1"
                     /translation="MQPIQHISVIGAGAWGTALAKHLAEKGLQTILWAYERDVLESIA
                     SKHENQLFLPGVTLPASLRVTNTLAEALEQCDGLLFVIPSHVARQVLQQLAPLLSRPL
                     PIISATKGVEEDTFKLMTQVVTEVLPPDLHDGLMVLSGPSFAMEVSQGQPTALCLAGT
                     NAALVGAFQAAFITPTLRIYADSDVVGVQLGGALKNVMALAAGVVDGLGLGHNTRAAM
                     ITRGLAEMVRLGMAMGADPRTFYGLSGVGDLVLTCTGTLSRNHHVGVRLGQGDQLDAV
                     LAGMQAVAEGVRTSRAAAGLALRHGVEMPIVREVNAVLFEGKSCRKAVSDLMERVAKP
                     EKGHP"
     gene            2689..>3350
                     /locus_tag="VFR82_01530"
     CDS             2689..>3350
                     /locus_tag="VFR82_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4500309.1"
                     /translation="MYTMQTITALIGATFLAISAPAALSLSMAQAETYVAGQFGVTIP
                     SIGKGLTDVDLTGSFIPGSTTSDQALKSSIMYGGKIGHYFRAAPWFGLEAEIYRTTPH
                     IKQQTVEFRGPNGPVGSAELAGVHMSVLTLAPLNLSFRYHKTRLQPYFAIGPGIFVAK
                     IKDPALSSDNSQSSTKLGLNAQLGLRYYLTRHVTMFAEGKYNHVRFNFPETPPGSSFN
                     LFG"
BASE COUNT          717 a         1070 c          902 g          661 t
ORIGIN      
        1 cctgagtcta tagatctcca tgctgggaac cataccggcc actgaaacgc caccaccctg
       61 cttccatctc actcgtgcac tcctcgcatc caccctggcc tcctgtatct tcctgctgca
      121 tccggttgcc ctttcggcga aatcaaagcc gacaagggct ccgcgtccgg agccagaatt
      181 gaaggttctt gagttgaaga tcagccccaa tccttacacc gtgtcagccg ggtccctgga
      241 attttcaacg gtcgttcaat tgccccgaga actagatggg gcgaccattt tggaagtgtc
      301 ttcactggtc acctctccct ccaaacgatc catccgcttt ctttccctgc ggaagccgct
      361 cgaatcgccg gcaatcacgc ctatcagcac cgatcgacag acgattcccg tcgtactgac
      421 ctgggatggc atggaccaca cgaaagcgcc ggcggaggcg ggaatctatc aattcgaact
      481 gcgggcgaag ctcctttcaa acggcgaaaa gggcccgcgg acgctcatgc tctcttggcc
      541 aaaacgcggc accctggaag tgaagtaact cgtcgccacg gctggaacat cagcgtgacc
      601 gtcttacttt atcccgaagg cgttgatgcg gtgcgccaga cagccggccc cgaacccgtt
      661 gtcgatattc atgaccccga cgccggccgc acaagaattc aacatggtca acagtgcggc
      721 taatccgttg aagcttgccc catacccgcg gcttgtcggc accgccacga caggacacgc
      781 caccaggcct ccgacaacgc tcggcaagac accgtccata cccgcgacta ccacaatgac
      841 gtgggcgtct gtgagctgct gatgccgacc cagcaagcga tgaataccgg cgacgcctac
      901 gtcgtaaagc gtctcaactc ggctgcccat gacctccgcc gtaatacggg cttcctcggc
      961 gacggggaca tcggacgttc cagccgtgat gacgaggacg cggccatgga gccgtcgctg
     1021 cgcatctcgt acggcgacga tgcgggcgga ttcgtggtag accgcccgcc gatcgatccg
     1081 tttgatgacg gccgctatgt cgcgagtcgc cctcgtcgcg agaaacggcc gatggtgttt
     1141 caacagcgag cccgcaatac cgcggatctg tgccggcgtc ttgccttcgc ataagatcac
     1201 ttcgggaaat ccctgacgga tcgaacgatg atgatccaac gaagcaaaac ccaagtcttc
     1261 gaagggaagg gatcgaagct gctcaagggc gctggcgatg gaagccttgc cgctcttgac
     1321 ctcgcggagc aagcgctcaa gccgttcacg attcatgggt gccccttttc cggtttggcc
     1381 acccgctcca tgagatcact gaccgctttg cggcacgatt tcccttcaaa gagcacggca
     1441 ttgacttccc ggacaatcgg catttcgaca ccgtgccgca gcgccaaccc ggcagccgcc
     1501 cggctcgtcc gcaccccttc cgccaccgcc tgcatccccg cgagaacggc gtccaattga
     1561 tcgccttggc cgagacgaac gcccacatgg tgattacggc tcaaggtacc ggtgcacgtc
     1621 aacaccagat cgccgacgcc tgacaagcca tagaaggttc ttggatccgc tcccatcgcc
     1681 atgccgagcc tgaccatttc cgccagacct cgggtgatca tggcggccct cgtgttgtgc
     1741 cctagcccca acccgtccac aaccccggcc gctaacgcca tgacattctt gagcgcccct
     1801 ccgagctgga ccccgacgac gtcgctgtct gcatagatac gaagggtggg cgtaataaaa
     1861 gcggcctgaa acgctccgac gagcgcggca ttggttccgg ccagacacag ggctgtgggc
     1921 tggccctggc tgacttccat ggcaaagctg ggccccgaga ggaccatcaa cccatcgtgc
     1981 agatcaggcg gcagcacctc ggtcacgact tgcgtcatga gtttgaacgt atcttcttcg
     2041 acgcctttcg tcgcgctgat gatagggaga ggccgagaga gcaagggggc gagttgttga
     2101 agcacctgcc gagcgacatg ggacgggatc acaaagagca ggccgtcaca ttgctccaag
     2161 gcctccgcca gggtgttggt cacccggagt gaggcgggaa gcgtcactcc cggcaagaag
     2221 agctggtttt cgtgcttgga cgcaatcgat tcgagcacgt cccgttcgta tgcccacagg
     2281 atagtctgta gacccttctc cgcgagatgc tttgccagag cggttcccca cgctccggca
     2341 ccgatgaccg agatatgctg gatcggctgc atagtcctgg atgaagggct caaggtggat
     2401 gggctgatgc cgggtttgcg ctgtgtgatc aggcgcgcgg attataggaa ggccatgttt
     2461 ctcctgtcaa cgaaccggcc gtccacggat tgtacggcac cgactgtttc cgatgacagc
     2521 cgaaagcaat ggccgacctg aaaacgaact ttctgcgctc tttccacctt tttctcgagg
     2581 cacaccgatg gctcaaacca cagtcggaat ttcacaaact tcttcgtgcc aggccgcccg
     2641 acggatagga tgggacaggc gatccatacc atgagtgagg acaggacgat gtacaccatg
     2701 caaacgatta ctgcgctcat tggagcaacc tttctcgcca tcagcgcacc ggccgctctg
     2761 tcattatcga tggcacaggc cgaaacgtat gtggcaggcc agttcggcgt gaccatcccg
     2821 tccatcggca agggcctgac ggatgttgat ctgacgggca gctttatccc cgggtcgacc
     2881 acatctgacc aggcccttaa atcatccatt atgtatggag gaaagatcgg acattatttc
     2941 agagccgccc cctggttcgg ccttgaagca gaaatctatc gcacgactcc ccacatcaaa
     3001 caacaaaccg tcgagttccg cggtcctaat ggtcctgtcg ggtccgccga gcttgccggc
     3061 gtccatatga gcgttctcac acttgcaccg ctgaatctca gcttccgtta tcacaagacc
     3121 agactccaac cgtacttcgc aatcggtccc ggaattttcg tcgcgaagat caaagatccc
     3181 gccctcagtt cggataattc gcagtccagc acgaaacttg gactcaacgc gcagctcgga
     3241 ctccgttatt acctcaccag acatgtcacg atgttcgccg aaggaaaata caaccacgtg
     3301 cggttcaatt tcccggaaac tccgccaggc agcagtttca atctgttcgg
//
LOCUS       DASTQY010000047        10529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_794652, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000047 DASTQY010000000
VERSION     DASTQY010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10529
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794652"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..188)
                     /gene="tatA"
                     /locus_tag="VFR82_01535"
                     /pseudo
     CDS             complement(63..188)
                     /gene="tatA"
                     /locus_tag="VFR82_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            complement(288..1499)
                     /locus_tag="VFR82_01540"
     CDS             complement(288..1499)
                     /locus_tag="VFR82_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU4500310.1"
                     /translation="MPIMLDLAHEQRIQEIQRVLREHDSSDGWLFYDFRGSDILAYRV
                     LLLDPRTHVTRRWYYWVPAHGTPQKLVHGIEPHVLDPLPGHMITYVSWDQQRQALARL
                     LTGNHRIAMQYSPMNAVPYVSRVDAGTIELIKSFGVDVVSSADFIQLFEARWTDRQLE
                     SHQYAAAALRRIVDDAFGHVSQCLTHGTGLTEYGLQQFILSRIKDAGMMTSSAPIAAV
                     NEHSADPHYGPAAIGSSIIERDNLVLIDLWAKQADAGSVYADITWTAYAGSTVPEKHR
                     AVFDVVRRGRDTALEFVRENVRAGRRPFGHEVDAACRRVIQVAGYGDQFIHRTGHSIG
                     EEVHGNGANIDGLETHETRRLLPRTCFSIEPGVYLAGEFGIRSELDVYLAEDDALVFG
                     LPLQTALSPLL"
     gene            1566..2018
                     /locus_tag="VFR82_01545"
     CDS             1566..2018
                     /locus_tag="VFR82_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500311.1"
                     /translation="MPMDSEASQFRITLFIGPQPVKGKPFTHSTVYNVKKRSWKGGVQ
                     VAVELEESQINFLSSSIGFPQWLDQTLEQVPVEERPAYQERAQDLFVQALAWCKLDLL
                     LQTGITQNNQCITSDTFVTELNMVAINKRHFVASHVATELDLLPHHPT"
     gene            2163..2594
                     /locus_tag="VFR82_01550"
     CDS             2163..2594
                     /locus_tag="VFR82_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500312.1"
                     /translation="MKLHVCAYTAATFRLVAVALVLGLCTVTVVPSAWSQEPLPPSSE
                     TTEGGNASSAGMGAAAGVSTLLYLPLKAAFAIGGGIVGGLAYAFSGGNETAAKSIWTT
                     SLYGTYIITPDHLRGDRPIRFLGVPETEASAQAAAPMEPIR"
     gene            2598..3338
                     /locus_tag="VFR82_01555"
     CDS             2598..3338
                     /locus_tag="VFR82_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU4500313.1"
                     /translation="MKPLIGVTPDFNAGDRKEWGGREPTYFLRARYVRAIEDLGGIPI
                     VLPLVADLGARRRLLTHIDGLLLTGSGPDLAPELYGERQRYKFRVMSQRRTTFELEMA
                     HLARAANMPVLAICGGMQALNVAFGGSLYQDIAAQRSKPLQHKQPTPATRLSHDVTIT
                     SGSLLRRIVKTGTMRVNSSHHQSVKDVAPSLMASAVAPDGIIEAIESPSQRFFLGIQW
                     HPEFLFDQHKLHRRLFEAFLRAARKGTT"
     gene            3428..4894
                     /locus_tag="VFR82_01560"
     CDS             3428..4894
                     /locus_tag="VFR82_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU4500314.1"
                     /translation="MARPAADSRPVISQLFRTKSIDQILADADHPTHRLKKSLTAWDL
                     TCLGIGAIIGTGIFVLIGTAIVGDAHRPGAGPGIVLSFVLSGLTCALAALCYAEMSAM
                     IPVAGSAYTFSYATLGEMLAWLTGWNLILEYGVACVAVAIGWSGYFNNLLRLAGLDLP
                     QWATRPPGGADGGIANLPAAIIVLLVTIILVVGIKESARATGIVVLIKLAVILFFLGI
                     GSTSVNPQNWTPFMPQGMAGVGAAAAIVFFAYIGFDAVSTAAEEARNPARDVPIGIIG
                     SLSLCTFLYIAVAAVLTGLIPSSQIDIHAPVAEALRLAGFKWGAAVIAIGAVAGITSV
                     LVVMMLGQIRVFFAMSRDRLLGPWLAAVHPRFGTPHRATILTGVAVAILAALIPIGEA
                     ADMTNIGTFFAFVLVCISVIVLRYTKPDHPRPFRLPFMPVVPILGTMACLGLMWQLPQ
                     LTWIRFGVWTVIGIVVYLGYGFKHSRLSVKALKQTAPF"
     gene            complement(4964..5371)
                     /locus_tag="VFR82_01565"
     CDS             complement(4964..5371)
                     /locus_tag="VFR82_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500315.1"
                     /translation="MANLLEYVGSVHIYLGPYRGNPIALYLKRSDAGCQIGPKVYPWN
                     DVVGMGDSPNKAAADFEEKWKANGLSADMYSGPSWEGGIKPEKPAPPKPPATPKPAAQ
                     ASSAPASQAPAAPAAPTQDQVSTPSGRTSGESS"
     gene            complement(5481..5957)
                     /locus_tag="VFR82_01570"
     CDS             complement(5481..5957)
                     /locus_tag="VFR82_01570"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEU4500316.1"
                     /translation="MQDRREVMKRWCGVMEWLDRLGYLTAGFSLLVLGMLIFAHAWYV
                     FITKAGQSGLLPSGLKLLNDLLLVIILLELFRTVIRFLQTEVLDLEPYLAVGIIACTR
                     RVLTASAELSHLPNMTETQFYQYLMDVGLNVAVIMVLVVGVFLVRKRPAQALPISP"
     gene            complement(5980..6366)
                     /locus_tag="VFR82_01575"
     CDS             complement(5980..6366)
                     /locus_tag="VFR82_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500317.1"
                     /translation="MGVKKGDLIDERQRHPDSCGLVVKVMDGGEGFEKIVCCDHELTE
                     QDVVPAMVSSKGRKRGQLATGVVLDEKKLYPDSCGLRVMIMDGGAGFREIRCCGHSLT
                     VSSKRELRYDQMRGSGPLQNGPEGNA"
     gene            complement(6392..7525)
                     /gene="nagZ"
                     /locus_tag="VFR82_01580"
     CDS             complement(6392..7525)
                     /gene="nagZ"
                     /locus_tag="VFR82_01580"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU4500318.1"
                     /translation="MTVASLELTSREKIGQLFMIGFMGTTVTPDLSAFIKNYKPGGVI
                     LFSRNLESVEQIVQLTNDLQQCSPKSPLLISIDQEGGRVSRLPQGFTIFPPCELIGRC
                     NSSELAYAAAATIAKELKAVGINMNMAPVLDVNSNPDNPVIGDRAFSSSAHAVGEMGL
                     ITAAGLQDNDVVACGKHFPGHGDTNADSHKELPVVDAPLERLEAVEFPPFRRAAAAEI
                     ATMMTAHVLYRALDEELPATLSPAIISGLLREQIGYDGVVLTDDLEMHAIVDHYGPGD
                     AAVRALLAGCDVLLICKERDREIAAFEAVEKAVASGTVTTARLDQSVARIQRVKQRYV
                     TPYRPAMISEAKLIAGCRTHQALLQSLRQASHRMEKSPAARTL"
     gene            complement(7522..9033)
                     /locus_tag="VFR82_01585"
     CDS             complement(7522..9033)
                     /locus_tag="VFR82_01585"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEU4500319.1"
                     /translation="MKIMQIDAKVGRAETEASEMLVLTHCEEERLNKQDASALDKALG
                     GTLSDLLQSKEFEGRAGEILVYHTQGKVPSKRLLFVGLGKKNNLTLDAIRQAMGHAVK
                     RVRQTKAGSFAAIVPAWTPRGHSALEVAQAMTEGAILGNYQFTAYRSDNGTPTEVGRM
                     TLVAPQKAQLRQLGEGIRRGVATAEAAVLVRDLCNHPSNVMTPSRIANEAKKVAKEET
                     LNLKILEQRDMEQLGMGALLGVARGSHEPPKFIVLEYNGSKKKNERPVVFIGKTITFD
                     TGGISLKPAENMEHMKADMTGGAEVLATVRAAARLQLPLRLVSILPVAENMPGGRAMK
                     PGDIVKTLSGKTVEVQNTDAEGRLILADALTYAQRYNPAALVDIATLTGACVVALGQF
                     AIGLFGTDEKLKAQVKKSGDKAGERVWEMPLWDEYFEQLRSDVADMRNIGGRGGGMIT
                     AALFLSKFAGNGPWAHIDIASTDWSERERAYIPKGPTGIGTRLLIQYLIDRSL"
     gene            complement(9103..9738)
                     /gene="queE"
                     /locus_tag="VFR82_01590"
     CDS             complement(9103..9738)
                     /gene="queE"
                     /locus_tag="VFR82_01590"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEU4500320.1"
                     /translation="MRVTEIFHSIQGESSYVGQPCVFVRLTGCPLRCTWCDTEYAFYG
                     GKEQSLKDILSQVEAYGCELVEVTGGEPLAQPDAVPLISALCERGHRVLVETSGAIDI
                     APIDNRAHVILDVKCPGSGMAGRMYWPNLDRLTMKDEAKFVLADRTDYEWARDILAQH
                     ALPGRCTVLMSPVFGTLDPRQLAEWVLADRLPVRLQIQLHKLIWTPDMRGV"
     gene            complement(9741..10451)
                     /locus_tag="VFR82_01595"
     CDS             complement(9741..10451)
                     /locus_tag="VFR82_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEU4500321.1"
                     /translation="MQGYHDLHPSLVRAATTASPASLAALFDVDNTLVPGEACEVRFF
                     RYLWRRGLVGWRELRRSVSWLIRQGRSVSIHPLRERKIYLEGKNSALIESCAREFFQR
                     EMVRNISTKGRDRLEAHRQSGHQLILVSGAPDFLITPLAEALNVSTLFAARIECRGGC
                     YTGAVIPPLPYGIGKRQLVVSHAQEKGLDLTSSFAYGDSPGDTEILELVGYPVVVNPI
                     RGMLRIARDRGWPVVDWT"
BASE COUNT         2181 a         3183 c         2911 g         2254 t
ORIGIN      
        1 ggggccgtcg tggacacggc ttctgcctgg ggcgcttccg gagaagggat atcgtgaacc
       61 tctttcttga atcctttcag cgccttgcca actccttcgc cgatctgagg aagcttgcct
      121 gcaccaaaaa tgatcagcac aatgaccaga atgatgatca attctgaaat gcccatggtc
      181 ccgaacatgc tctaattcct tcggtttcag gagtatcggc ccaaaagcca aaagtcactt
      241 tataggccgt ttgacagcac tgtcaaggaa gggcgcgccc gaccggatta cagcaacggg
      301 gaaagtgcgg tttgcagcgg aagcccgaac acgagcgcat cgtcttccgc cagatacacg
      361 tccaactcgc ttctgattcc aaattcgccc gccaggtaga cgccgggctc aatcgaaaaa
      421 caggtgcgcg gcagcaggcg tctggtctcg tgggtttcta gaccgtcgat gttcgccccg
      481 ttcccgtgca cttcttcgcc gatggagtga ccggtacgat gaatgaattg atctccgtat
      541 cccgccactt gaatgacgcg gcgacacgca gcatcgactt catggccgaa cggtcggcgg
      601 ccagcccgta cgttctcccg cacaaattca agcgcggtgt cccgtcctct tctgactaca
      661 tcgaagacag cgcgatgctt ctcgggaacc gtcgaaccgg cataggcggt ccaggtaatg
      721 tcggcataca cggagcctgc gtcggcctgt tttgcccaca ggtcgatgag cactaaatta
      781 tctcgttcaa tgatcgatga tccgatcgct gcaggaccgt aatggggatc cgcgctgtgc
      841 tcattcaccg ccgcgatggg tgcgctggac gtcatcatgc ctgcgtcctt gatccgtgaa
      901 agaatgaact gctgtaagcc gtactctgtg agccccgttc cgtgagtcag gcactgactg
      961 acatgcccaa atgcatcgtc cacaatgcgc ctcaatgctg cggctgcgta ctgatgcgac
     1021 tccaactgtc ggtcggtcca gcgtgcttcg aatagttgga tgaaatccgc cgaactgaca
     1081 acgtcaactc cgaagctctt aattaattca atggtgccgg catccaccct agacacataa
     1141 gggaccgcgt tcatgggcga atattgcatc gcaatccggt ggttgccggt aaggagacga
     1201 gcgagcgctt gccgctgctg gtcccacgac acataggtaa tcatatggcc gggtagggga
     1261 tccaatacat gcggctcgat tccatggacc aatttttgcg gtgtgccatg agccggaacc
     1321 cagtagtacc agcgcctggt cacatgtgta cgcggatcca ggagaagcac acggtaggcg
     1381 aggatatcgc ttccacggaa atcataaaag agccacccgt cgctggaatc atgttcgcga
     1441 agcacccgtt ggatctcctg tattcgctgt tcatgcgcga ggtccagcat gatcggcatc
     1501 ttatcgttgt cgttttggag cgtcaatcaa gagggattgc catcggccat ggtagaatgc
     1561 gcagcatgcc gatggactcc gaggcatctc aatttcgcat aacattgttc attggccccc
     1621 agccggtgaa ggggaaaccc ttcactcact cgaccgtgta caacgtcaaa aagcggagtt
     1681 ggaaaggtgg cgtccaggta gccgtcgaac tcgaggaatc tcagatcaat tttctgagtt
     1741 cgtccatcgg ctttcctcaa tggctggacc agacgcttga gcaagttccg gttgaagagc
     1801 gtcccgctta ccaggaacgg gcgcaggact tgtttgtcca agccctcgca tggtgcaaat
     1861 tagacttatt gttgcaaacc ggcatcactc agaacaatca atgtatcacc tccgacacat
     1921 tcgtcaccga attgaacatg gttgctatca acaaacggca cttcgtcgca tcacatgtgg
     1981 cgacagaact tgatctccta ccgcaccatc ctacttgatg tgcgagttct cccggcctgg
     2041 tagggggttt gcaagcacag tgtaatttgt tataacccac agcacgctgc acttcgactg
     2101 agcgtccaag gaattccacg gatgcagctc gattcacttt cttgaggaag gaggaccctc
     2161 tcgtgaaatt acatgtgtgt gcttacactg cggcaacgtt cagactcgtc gccgttgctc
     2221 tcgtcctcgg tctttgcact gtcaccgtag tgccctcggc gtggagccaa gagccccttc
     2281 cgccatccag cgagactacc gaaggtggga atgcctccag cgccggtatg ggcgctgccg
     2341 ccggcgtctc gaccttgctg tacctcccgt tgaaggcggc ctttgccatc ggcggaggca
     2401 tcgtcggcgg cctggcctat gcgttttccg gagggaacga aaccgcggca aaaagcatct
     2461 ggaccaccag cctctatggc acgtatatca tcacgcccga tcacctgcgc ggagacagac
     2521 ccatccgttt cctcggcgtc ccggagacgg aggcatcggc tcaggctgcg gcccccatgg
     2581 agcccattcg ctgagcaatg aaaccgctca tcggcgtgac accagatttt aacgccggtg
     2641 accggaaaga atggggcgga cgcgaaccca cctatttcct gcgcgctcgc tacgttcgcg
     2701 ccatcgaaga cctcggagga atcccgatcg tccttccgct ggtcgccgac cttggagcgc
     2761 ggcggcgttt gctcactcac attgacggcc tcttgctgac cggcagcgga cccgacctcg
     2821 ctcctgaact ctacggtgaa cgccagcggt acaagttccg ggtgatgagc caacgacgca
     2881 caacattcga gttggagatg gctcatttgg cacgcgcggc caacatgccg gtgctggcca
     2941 tttgcggagg catgcaggcg ctcaacgtcg ccttcggagg cagcctgtat caggatatag
     3001 ccgcccagcg gtccaagccg ctgcagcaca agcaaccgac tcccgcaacc cggctgagcc
     3061 atgacgtgac gatcacctct ggaagcctgt tgcggcgcat cgtcaagacg gggacaatgc
     3121 gagtaaacag ctctcaccat caatcagtaa aggacgtggc accatccttg atggccagcg
     3181 ctgtggcacc ggacggcatt atcgaggcca tcgaatcgcc gtcccagcgg ttctttctcg
     3241 gaattcagtg gcacccagaa tttctgttcg accaacacaa gctccatcgg cgactcttcg
     3301 aggcgtttct ccgcgctgcg cggaagggca cgacctagcc tatgccggcg accttccatc
     3361 tcctcgtgag gctgcctgct atacttgatg gaatatggag cgggaagacg ccctcagccg
     3421 accgtcaatg gcacgacctg ctgcggacag ccgaccggtg atctcccaac tgttccgcac
     3481 gaaatccatc gatcaaatcc tcgcggacgc cgaccatccg acgcatcgac tcaaaaagtc
     3541 cctgacggcc tgggatctca cctgtctggg catcggcgcg atcatcggga ccggcatttt
     3601 cgtgttgatc ggaacggcca ttgtcggtga cgcccatcga ccaggagccg gccccggcat
     3661 cgtcctctcc ttcgttctgt ccggactgac ttgtgcgctc gccgcgctct gctatgcgga
     3721 aatgtccgcg atgataccgg ttgccggcag cgcctatacg ttttcctatg cgacgctcgg
     3781 ggagatgctg gcctggctca ccggctggaa tctcattttg gaatacggcg tggcgtgtgt
     3841 cgcagtggcg atcggctggt ccggatattt caacaatctt ctgcgactgg ccggtctcga
     3901 cttaccgcag tgggcgaccc gtccgcctgg tggcgccgac ggaggaatcg ccaaccttcc
     3961 ggctgccatc atcgtgctgc tggtcacgat catccttgtc gttggaatca aggaaagcgc
     4021 acgcgcgacg ggtatagtcg tgctcatcaa actcgccgtc atcctgttct tcctcgggat
     4081 cggcagcacc tcagtcaatc cgcagaactg gacgccgttt atgccgcaag gcatggcagg
     4141 ggttggggca gccgcagcaa tcgtgttctt tgcctacatt ggcttcgatg cggtctctac
     4201 agcagcagaa gaggccagga atccggcccg tgacgtcccg atcgggatca tcggatcatt
     4261 aagcctctgc acgttcctgt atatcgctgt cgccgccgtg ctcacggggc tgattccctc
     4321 atcgcaaatc gatatccatg ctccagttgc cgaggcactc agattggcgg gcttcaagtg
     4381 gggggccgca gtcatcgcga tcggcgcggt cgccggcatt accagcgtgc tggtcgtcat
     4441 gatgctgggc cagattcgag tgttctttgc gatgtccagg gatcggctgt taggcccctg
     4501 gcttgcggcg gttcaccctc ggttcggaac tccccaccgc gcgaccattc tcaccggtgt
     4561 cgcagtcgcg atattggcgg cgctcattcc aatcggcgag gcagccgaca tgacaaacat
     4621 cggcacattc ttcgcgttcg tgctcgtctg tatcagtgtg atcgtgctgc gatataccaa
     4681 acctgatcat cccaggccgt ttcgcctgcc gttcatgccg gtggttccaa ttcttggcac
     4741 gatggcctgt ctgggactca tgtggcaact cccccaattg acgtggatcc gcttcggcgt
     4801 ctggactgtg atcggcatcg tcgtctattt aggttatggc ttcaagcaca gccgattgtc
     4861 ggtaaaggcg ttgaagcaaa ccgctccatt ctgacgggaa gcgatctaaa agacaggccc
     4921 gccactattg tcagcctggt tgaacggtag ggtactctga attctagctt gattcgccgg
     4981 acgtccgacc ggaaggcgtc gaaacctggt cctgagtcgg agccgcaggc gcagcaggag
     5041 cttgcgaggc aggagcagag ctcgcttgag cagccggttt gggagtggct ggtggcttgg
     5101 gaggagccgg tttttcgggt ttgatgccgc cctcccatga cggccctgaa tacatatcgg
     5161 ctgaaagacc attggctttc catttttcct cgaaatccgc cgccgcctta ttgggcgaat
     5221 cccccatgcc gacgacgtcg ttccaaggat agactttcgg gccgatctgg cagcctgcat
     5281 cagagcgttt gaggtacaag gcgatcggat ttcctctata gggcccgaga taaatgtgca
     5341 cagaaccgac gtattcaagc aagttcgcca tgccggtctc cagggagggg catgatgcca
     5401 taagccgaaa taggaggcaa gcagagggct caaaggcgcc ggctgatctg gaggcgatca
     5461 cttgcaagga ggattggaga ctagggcgag atgggaagag cttgagccgg acgtttgcgg
     5521 acaaggaaca cgcccacgac gaggaccatg atcaccgcaa cgttcaaccc gacatccatg
     5581 agatattgat agaattgagt ttcagtcatg ttcggcaggt ggctcaattc agcactggcc
     5641 gtaaggacac gcctggtgca ggcgataatg ccgacggcca gatacggctc cagatccagg
     5701 acttcagtct gcaggaatcg gatgaccgtg cggaacagct cgaggagaat gatgacgagt
     5761 agcagatcgt tcaaaagttt caggccggaa ggcaacaacc cactctggcc cgctttcgtt
     5821 atgaagacgt accaggcatg ggcgaagatg agcatgccga gtacgagcag actgaagccg
     5881 gctgtcagat agcccagccg atcgagccat tccatgactc cacaccagcg cttcatgact
     5941 tccctacggt cctgcatggc ggccctccct gacgttggac tatgcattcc cttccggccc
     6001 gttctggagc ggacctgaac ctctcatttg gtcatagcgc aactcccgct tggaactgac
     6061 cgtcaacgaa tggccgcagc accggatttc tctgaagccc gctccgccgt ccatgatcat
     6121 gacccgcagc ccgcaggagt cgggatacag tttcttttca tcaaggacga cgcccgtcgc
     6181 cagctggcca cgcttacgcc ctttcgaact caccatcgcc ggcaccacat cctgctcggt
     6241 caattcatgg tcgcagcaga cgatcttctc gaacccttcc cctccgtcca tgaccttcac
     6301 aacgaggccg caagagtcgg gatgccgctg acgttcatcg atcagatctc cctttttgac
     6361 acccatgatc gacccgcgtt cctttgatca actagagggt tctcgctgcc gggcttttct
     6421 ccatgcgatg actcgcctgg cgcagactct gaagaagcgc ctggtgcgtc cggcagccgg
     6481 caatcaactt cgcctcggag atcatcgccg gccggtacgg tgtgacatag cgctgtttga
     6541 cgcgttgaat ccttgcgacg gattgatcca acctcgccgt cgtcacggtt cccgatgcca
     6601 cggccttctc gaccgcttcg aaagccgcga tctcccggtc acgttcctta cagatcaaca
     6661 acacgtcgca ccctgccagc agagctcgaa cggccgcatc ccccggaccg tagtggtcca
     6721 cgatcgcatg catctcgagg tcgtccgtca gaacgacacc gtcatatccg atctgttccc
     6781 tcaggagacc gctgatgatg gccggagaga gcgttgccgg aagctcttca tcgagcgccc
     6841 tgtacaagac atgcgcggtc atcatcgtag cgatttcagc cgccgcagcc cggcgaaacg
     6901 gcggaaactc taccgcctct aatcgctcca gcggagcatc aaccaccggc agttccttgt
     6961 gagagtccgc attcgtatcc ccatggccgg gaaaatgctt gccgcatgca accacatcgt
     7021 tgtcttgcaa tccggcggcc gtgatcagcc ccatttcacc gacagcatga gccgaagagc
     7081 tgaacgccct gtcgccgatc accggattgt cgggattgct attgacgtcg agaactggcg
     7141 ccatgttcat gttaatgcca acggctttga gctccttggc tatcgtcgcc gcagccgcat
     7201 aggccagttc actggaattg caccgtccga tgagctcaca gggaggaaag atggtaaagc
     7261 cttggggcag gcgggacacg cgcccccctt cctgatcgat ggagatcaag aggggggact
     7321 tcggactgca ttgttggaga tcgttggtca gctgaacaat ctgttcaacg gattcgagat
     7381 tccgtgagaa caggatcacg ccgcccggtt tatagttttt gatgaatgca gacaggtccg
     7441 gtgtgacggt ggttcccatg aacccgatca tgaagagctg cccgattttt tcccgcgacg
     7501 tcagctcgag actggccaca gtcacaggct ccgatcaatg agatattgaa tcaagagcct
     7561 tgtaccaatg cccgtcggcc ccttgggaat atacgctcgc tctcgctcgc tccaatcggt
     7621 actggcaata tcgatgtgcg cccaggggcc attgccggcg aacttactca ggaacagagc
     7681 ggcggtaatc atgccgccgc cgcgaccgcc gatattgcgc atatcggcga catcactgcg
     7741 aagttgctcg aaatattcat cccacagcgg catctcccac acgcgttcac cagctttgtc
     7801 gcccgatttt ttgacctgcg ccttcagttt ctcatctgtg ccgaatagcc cgatggcgaa
     7861 ttggcccaag gcgacgacgc aggccccggt cagcgtggcg atatcgacca atgcggccgg
     7921 gttgtagcgt tgggcgtacg tgagtgcatc ggcgagaatt agtcgccctt cggcgtccgt
     7981 attttgcact tcaactgttt tccccgacaa cgtcttcacg atgtcaccgg gcttcatggc
     8041 tcggccgcca ggcatattct ccgcaacggg tagaatgctg acgagccgca gaggcagctg
     8101 aagacgtgct gcggccctca cggtagcgag cacttcggcg cctccggtca tgtcggcctt
     8161 catgtgctcc atgttctcag ccggcttgag agaaattccc cccgtgtcga acgtaatcgt
     8221 cttcccgatg aagacgacgg gacgctcgtt ctttttcttg gagccgttgt attcgagcac
     8281 gatgaacttc ggcggttcat ggctgcctcg tgcaacgccc aacagagcgc ccatccccag
     8341 ctgttccata tcccgttgtt cgaggatctt gagattcagc gtctcttctt tcgccacttt
     8401 cttggcttca ttggcaatgc gggacggcgt catgacgttt gagggatgat tgcagagatc
     8461 ccggaccagc acggcagcct ccgcagttgc cacgccgcgg cggatgcctt cgccgagttg
     8521 ccgcaactga gccttctgcg gggccaccag cgtcatccgg ccgacctccg tcggcgtgcc
     8581 attgtcgcta cgataggcag tgaactgata attaccaaga atcgcccctt ccgtcatggc
     8641 ttgcgcgacc tcgagagcag aatgtcctct cggagtccac gccggcacaa tcgcagcaaa
     8701 ggaaccggcc ttcgtctgcc gcacccgttt cacggcatga cccattgcct gacggattgc
     8761 atccaacgtt aagttgttct tcttgcccag tccgacgaag agaagacgtt tcgacggcac
     8821 cttgccctgc gtgtgataga ccagtatttc tccagctctc ccttcgaact ctttcgactg
     8881 cagcaggtcg cttaatgtgc cgcccagggc tttgtcgagt gcggacgcgt cttgcttatt
     8941 gagacgctct tcttcgcaat gcgtgagcac gagcatttcg gacgcttccg tttccgcgcg
     9001 cccgacctta gcgtctattt gcatgatttt cattcggttc cgctccttgt cagtgatcag
     9061 tggtcgatga gctcctgtcc tgaaaatcag ggatgggggt gctcatacgc cgcgcatgtc
     9121 cggcgtccaa atcaatttgt gcaactgaat ctgcaatcgg acgggcaaac gatcagccag
     9181 aacccattcc gccaactgac gcggatcaag ggtaccaaac accggactca taaggacggt
     9241 gcagcggccc ggcagcgcat gctgggcaag aatgtcgcga gcccactcat aatcggtgcg
     9301 atcagcgagc acaaacttcg cttcatcctt catcgtcagc cggtcgagat ttggccaata
     9361 catccgtccc gccatcccgc tgcccggaca tttcacgtcc agaatcacat gcgcccggtt
     9421 atcgatcggt gcaatgtcga tggcgccact tgtctccacc agaaccctgt ggccccgctc
     9481 gcatagtgcc gagatcaacg ggacggcgtc aggctgcgcg agaggctcac cgccggtcac
     9541 ttccaccagc tcgcatccat aggcttcaac ctgcgagagt atgtccttta acgactgctc
     9601 ctttcctcca tagaaagcgt attcggtgtc gcaccaggta cagcggagcg ggcaaccggt
     9661 caaccggacg aagacacagg gctgcccgac gtaactcgat tcgccttgga tgctgtgaaa
     9721 gatttcggtg acacgcatgg tcaggtccaa tccacaaccg gccacccccg gtcgcgcgcg
     9781 atgcgaagca tgccgcgaat cgggttgacg acgaccggat accctacgag ctcaaggatt
     9841 tccgtatctc cagggctatc accgtacgca aacgaactgg tcaggtcgag acccttttcc
     9901 tgggcatgcg acacaacaag ctgccgcttc ccaatgccat acggaagcgg tggaatgacg
     9961 gcgccggtgt aacatcctcc tcgacattca atccgtgccg caaagagcgt tgacacgttc
    10021 agcgcttcag caagcggcgt gatgaggaag tccggcgcac ccgagaccaa aatcagctga
    10081 tggcccgact gtcgatgcgc ttcgagtcgg tcacgtccct tcgtcgagat gtttctcacc
    10141 atttcgcgtt gaaaaaactc ccgggcgcaa gactcgatca gagccgaatt ttttccttcg
    10201 agatagatct ttcgctcccg gagtggatgg atcgagacgg accgaccctg tctgatcaac
    10261 caggacacac tgcgcctcaa ctcgcgccat cccacgagac cgcggcgcca caggtagcga
    10321 aaaaaccgaa cttcacaggc ttctcccggc acgagcgtgt tatcgacgtc gaagagggcg
    10381 gcaagcgatg caggggatgc agtcgttgca gcccgaacga gtgacggatg aagatcgtga
    10441 tagccttgca tggtattggg tcaactggcg agcgacagac ggccaatatt accggacagg
    10501 ccattgattg acaaggattt tggcctact
//
LOCUS       DASTQY010000048         6016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1176452, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000048 DASTQY010000000
VERSION     DASTQY010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176452"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="VFR82_01600"
     CDS             <1..1208
                     /locus_tag="VFR82_01600"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEU4500322.1"
                     /translation="KIIPEEILKPVAVGRLVLDRMPDNFFAETEQVAFMTQNVPPGID
                     FSNDPLLQGRNFSYLDTQLKRLGSVNFTHLPINAPKCPFAHFQQDGHMAMRNPMGRVN
                     YQPNSWGEGPRESPERGHRPFPEEIEGTKERVRSETFADHYSQARQFFVSQTKTEQDH
                     IVAALTFELSKVETPAIRERMFAHLLNIHDELANKVAQGLGLKKLPNPADAAMPTRGD
                     LEPSPALSIIRNGPNRFEGRKLGILVTDGTDAALLKALQAAIHKAGALTEIIAPKVGG
                     VKASDGSWIEAHQMIDGGPSVLYDAVAVLPSEAGTAQLLQHAPARDFVADAFQHCKFI
                     GHTSAAQPLLDKAGVQPDKGVVPLTGKVVDSFVGALGALRIWDREPKVRLQPEPPARP
                     EPSHGSSGP"
     gene            1291..2229
                     /locus_tag="VFR82_01605"
     CDS             1291..2229
                     /locus_tag="VFR82_01605"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEU4500323.1"
                     /translation="MPESLPRTKEERRLKRAARREKQRQANIGRAREMHLARPGREKN
                     MDPLPTIAGDSKTRQLFIGCSGWFYWHWRGAFYPADLPTNRWFPHYAGRFKTVELNAP
                     FYSWPTVATVESWIRQAGRRQFVYTVKVCELITHVKRFAGTKTLVRDFGLIADLLGPR
                     MGCFLFQLPPSFRYTPARLKRILDQLDHRRRNVVEFRHKSWWNEAVHKAFRETGTIMC
                     SCSGPRLPDELVKTAEDIYIRFLGLKNWYRHDYSTAELAVWAKRIEESGARTVWAYFN
                     NDRDGYAIKNARALMRLLEPGTRRAKARNRQLVVHT"
     gene            2579..2806
                     /locus_tag="VFR82_01610"
     CDS             2579..2806
                     /locus_tag="VFR82_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500324.1"
                     /translation="MDRPEIGGAMTVADLIAKLSRYPESARVTLLDPSRRWLMPIEIT
                     ALLSADGMSREVDFIAITADSASDEIEGLAP"
     gene            2881..3243
                     /locus_tag="VFR82_01615"
     CDS             2881..3243
                     /locus_tag="VFR82_01615"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEU4500325.1"
                     /translation="MKDARKSDQSQAPGHFENQGPKEQAKPAAVPDLEERHQRTRDQA
                     PTDTGREDRIRQQTYEMWEREGQPEGLAHQHWERAAQEHDGEEKKTDSQAKGLAGEKP
                     GEQSGSKPSSDTSEPSRT"
     gene            complement(3605..4162)
                     /locus_tag="VFR82_01620"
     CDS             complement(3605..4162)
                     /locus_tag="VFR82_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500326.1"
                     /translation="MENIPQLILWLLLPAWIVLGFLDWLCHRRSEIEVHCGPWESVLH
                     LTLLALAGIAILMGLLLELTEPVLCVLALCFVLHEVTVYVDIRYAHARRDVSPAEQRV
                     HDFMTAVPVSVLCLIAILHWDTMVSLVFQPSSIVRAPIRLKTHPLPPAQVIGILVAVL
                     FANFLPYLEELLRGARALRAKLGDH"
     gene            4647..4877
                     /locus_tag="VFR82_01625"
     CDS             4647..4877
                     /locus_tag="VFR82_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500327.1"
                     /translation="MIRTRLIPTADAAVSSQTSVAGSIFAVGPSATIVLLGGELNAEI
                     EHQIAKDSTSGGNKPLGTRGAKMTDTVGAAQG"
     gene            5727..>6016
                     /locus_tag="VFR82_01630"
     CDS             5727..>6016
                     /locus_tag="VFR82_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500328.1"
                     /translation="MPALTIHTITSMRTDEAKDVVHIQCTTEKDTANLDVRSGALSTL
                     VLGLRQASEAFMSSGESSGQPLELTGTGLVSVEDGSIMLELIFDGSIRVIANV"
BASE COUNT         1365 a         1731 c         1747 g         1173 t
ORIGIN      
        1 ccaagatcat ccccgaggaa atactgaaac cggtggctgt cggccgcctg gtcctggacc
       61 gcatgccgga caacttcttc gccgagacgg agcaggttgc gttcatgacc cagaacgtgc
      121 cgcccggcat cgacttctcc aacgatccgc tgctgcaggg tcgcaacttc tcctatctcg
      181 acacccagct gaagaggctg gggagcgtaa acttcacgca cctcccgatc aacgcgccga
      241 aatgcccgtt cgcgcatttc cagcaggatg gccacatggc gatgcgcaac cccatgggcc
      301 gagtgaacta ccagccgaac tcgtggggcg aggggccacg cgagtcgcct gaacgcggtc
      361 accgtccctt cccggaggag atcgaaggta ctaaggagcg cgtgcgcagc gagacctttg
      421 ccgatcatta tagccaggcg cggcagttct tcgtgagcca gaccaagacc gagcaggacc
      481 acatcgtcgc ggcgctcacc ttcgagctca gcaaggtgga aacgccggcg atccgcgagc
      541 gcatgttcgc gcacctgctc aacattcatg acgagctcgc gaacaaggtc gcgcagggcc
      601 tcggcctgaa gaagctgccg aaccccgcgg atgccgcgat gccgacacgc ggggacctgg
      661 agccctcgcc agcgctcagc atcatcagga acggtccgaa ccgtttcgaa gggcgcaagc
      721 ttggcatcct ggtaacggac ggcacggacg ccgcgctgct caaggcgctg caggcggcga
      781 tccataaggc cggcgctttg accgagatca tcgcgcccaa ggtcggcggc gtgaaggcaa
      841 gcgatggcag ctggatcgag gcgcatcaga tgatcgatgg cggcccgtcc gttctctatg
      901 acgccgtcgc agtgctgcct tcggaggccg gcacggctca gttgctgcag catgctccgg
      961 cgcgggactt cgtcgccgac gccttccagc actgcaagtt catcggccac acatccgcgg
     1021 cacagccgct gctcgacaag gcgggggtgc aacctgacaa aggcgtggta ccgctcaccg
     1081 gcaaggtcgt cgacagcttc gtcggcgccc tcggcgctct gcgcatctgg gaccgcgagc
     1141 cgaaggtcag gctgcagccc gagcctcccg cccgccctga accaagtcac ggctcgtcgg
     1201 gtccctagca ttgaacagag gacgggtctg tcaccactat tagaaaccgc aaacgtttgt
     1261 aagccggcgt ttcggccaac gcgcggaagc atgcctgaaa gcctcccccg caccaaagaa
     1321 gagcgcagac tcaagcgggc cgcacgccgg gaaaagcaac gtcaggcgaa tattggccgc
     1381 gcccgagaaa tgcatcttgc gcgccccggt cgggagaaga acatggatcc tctcccgacg
     1441 atcgctggcg atagcaagac ccgccagctt ttcatcggat gctcaggctg gttctactgg
     1501 cattggcgcg gcgctttcta tcctgcggac ctgccgacaa accgttggtt cccgcactat
     1561 gctggacgct tcaagaccgt ggaactgaat gcgcctttct attcatggcc gaccgttgcc
     1621 actgtcgagt cttggatacg gcaggccgga agacgccaat tcgtctacac cgtgaaggtc
     1681 tgcgagctta ttactcacgt taaacgcttt gccgggacca agactttggt gcgtgacttt
     1741 ggcctgatag ccgatctgct tggaccgcgc atggggtgtt ttctgttcca gctaccgccc
     1801 agtttccgat atacgccggc gcgcctaaag cggattctgg accagcttga tcacagacgg
     1861 cgcaacgtcg tggaatttcg gcacaagagc tggtggaacg aagcagtcca caaagccttc
     1921 cgggagacag gaacaatcat gtgctcgtgc agcggtcccc ggctgccgga tgaactggtc
     1981 aagacggcgg aggacattta catccgcttt ctcggcctca agaactggta tcggcatgac
     2041 tattcgactg cggagttggc cgtctgggcg aaacgcattg aagagagcgg cgcgagaacc
     2101 gtttgggctt atttcaacaa tgaccgcgac ggatacgcca tcaagaatgc tcgcgcacta
     2161 atgcggctgc tagagccagg aacacggagg gctaaagcaa ggaatcgcca gctagtggtt
     2221 cacacctgac agcagacaaa ttcgccatga atggatctcc aagcctgaac aaaacgccca
     2281 gagccctgtt ggcgggtatg tctttgcttt tgtccgaaat cgtacggagc gtagttttaa
     2341 cctcgggaat ggcgaccgaa ctccttcccc ctcgcggtcg gtcgcaaggc ggcggagcct
     2401 cacattggcc cctgactgtg actggcttcg ccgccgcttt tgttgtccgc ggcaacacac
     2461 gtcgaacaca gctctttggg caaagaaggg tgcgttccag gcccggtgcg accgtggcca
     2521 agattgtccg cagtgagtcg agatcaatcc gctcttctca agcaagttga gtgctggcat
     2581 ggaccgaccc gaaattggag gcgctatgac tgtcgccgat ctgatcgcaa agttgtcccg
     2641 atacccggag agtgcccgag tgacgttgct tgatcccagc aggcgatggc ttatgccgat
     2701 cgaaataacg gcactactat cggcggacgg aatgagtcgt gaagtcgatt tcattgcaat
     2761 cacagcggac agcgcgagcg acgaaattga aggcctggcg ccctaagaag aggaaactcg
     2821 aagagctcgg cgcctatttt cttgccactt ggcgaaacca tttgaaacgc ggagtgcatc
     2881 atgaaggacg ctcgaaagtc agatcaatcc caagctcctg gtcacttcga aaaccagggg
     2941 ccaaaggagc aggcaaagcc tgcagccgtt ccggacctag aagagcggca tcaacgcacc
     3001 agagatcagg cgcccaccga taccggtcgc gaagacagga ttcgccagca gacatacgaa
     3061 atgtgggaac gcgaggggca gcccgaaggc ttggcgcacc aacattggga gcgcgcggca
     3121 caggagcacg atggcgaaga gaagaagacg gatagccaag ctaagggctt ggctggtgaa
     3181 aagccgggcg agcagtcagg atcgaagcct tcgagcgaca cttcagagcc tagccgcacg
     3241 tgaatctggt tgctcgcgat gcgcagtcga cagcatgacg taacgtgcgg gctttggtcg
     3301 ccgttcattc cagtatgaga ctagcgaact catcggtagc tcgccgataa acagcgaaac
     3361 ggacttcttc attcagtccc cctgctgcac aacttggatc gattgccgcg acctaggcga
     3421 attgctggct catgaggaat ggtgcaatcg agaccggaat gcctctccaa tgccgggacg
     3481 ggggtcgcaa tgagacccga ctctcctctc gcggcatgag ttcgaggccg ttctattttc
     3541 cgaagccaga aagccaacaa cattatccaa agtccaatcc ttccccgtag cagcaggttt
     3601 ttgatcaatg atcgcccaat ttagcgcgca gtgcgcgggc gccgcgcaaa agctcctcga
     3661 ggtatggcaa gaagtttgcg aacaggaccg ctactaaaat cccgatcact tgggcaggcg
     3721 gcagcggatg agtcttgagg cggatcggcg cccttacgat gctagagggt tgaaacacca
     3781 ggctcaccat ggtgtcccaa tgcaagatcg cgatcaagca cagtaccgag actggcacag
     3841 ccgtcataaa atcatgaaca cgttgctccg ccggcgatac gtcgcgacga gcgtgtgcat
     3901 atcggatatc gacatagacc gtcacctcat ggagaacgaa gcacagagca agaacgcaca
     3961 aaactggttc ggttaattcc agtagtaggc ccattaggat tgcgataccg gcaagtgcca
     4021 ggagagtgag atggagtacg ctttcccacg gaccgcaatg aacttctatt tcggagcgac
     4081 ggtggcaaag ccaatccaaa aaccctagaa cgatccacgc tggcaacagg agccaaagaa
     4141 taagttgggg aatgttctcc acgagagacc ttattgggat gggacatgtg aagtagacgg
     4201 agtttgaacc tcactcagca agacgtggtc gtggccgcta ataatgaact cctatggggc
     4261 gccgcgacgc agttcgcgtc aagatcatct gtgtcgttca ccctctatgt cgatcatcaa
     4321 cattaatacg tagccccggt tccgctggct cggccgtcac aagtgggccc ctttgggtgg
     4381 gtaaacacgt cgtatggcac gcgcggatgg catcgagaaa gttggatacc agtgattacg
     4441 aggtatccaa ctggattgcc tttatcaaaa gcaaattctc tacgattttt tcaaaccgag
     4501 gcagcggttt gacctagggt cgtgtttcca ccgggcgaac aaaccctatt ccaggagcct
     4561 ccccgtgtct ttcaatccac ttgaggaaag cggcttgccg ctcgagaagc agtttcgcaa
     4621 ttggcgcgat cttagcgtta cgccatatga taaggacgcg gctcatccct acggccgatg
     4681 ccgcggtatc gtcgcaaacg agcgtcgcgg gatcaatttt cgccgttggg ccgtccgcca
     4741 ccatcgtgct cctgggtggt gagttgaacg ccgagatcga gcaccaaata gccaaggaca
     4801 gcacatcggg cgggaacaaa ccgctcggca cgcgcggagc caagatgacg gacacggtag
     4861 gtgcggcgca gggctgacgc ctggcaccga cgcattcaat ccggtcaacg gccttgcagg
     4921 cacgacttgc cgtcagtggc gcgattgata cgaggcgtgt ttccgttgag cagagtgcag
     4981 tacgctaata agcgtaggcc acgtaggagc ctgcccttcc tttacttaca acgccgctca
     5041 agctgagttg acggctggcc aagccaacgc ttgagaagcg tggaacggag tcgaaatgtc
     5101 aatcatttac gctggatgca ttagcgtgat cgttcttgct ctactcggaa tatgggctac
     5161 cacggaagag atcatcaacg aactgcacga ccgccatcac taatgcatcc ctgaggcgct
     5221 ccaagcggtt tttatagatt aggtccggtc tcgagctgtc ggatcgaaag acacgcgacc
     5281 aacgcactgc cctttacacc aaacttcgac caacggcgtc gttgcgttca aaggaaagcg
     5341 gcttcggctc ttttttagag cttcatcgtt gtcatcggca tcgattgatt cgatgcctct
     5401 gacgctgtca ttgcaatcga ctatgaggca ctgatactgg gtcatgcgtg cgtccgttcc
     5461 ttcattcgaa cagtggtcgc ttggtctcgt tgcgtcgcac gtatggaagt gcaagtcagg
     5521 ttcgcgctcg cgcgaccctt aagaatcggg tggcgttagc cggtcttact tattggcccc
     5581 gcagcggtac ccgagaccgc gttgtcgaca agcgggccaa acgttgagcc gagacccacc
     5641 ggcctcatag acttgtcttt ttacaccctg cagccaggag tgtgatggtt tggactgccg
     5701 gttgtttgtc tcctcggagg tactccatgc cagcgctaac aatccacacc atcacttcaa
     5761 tgcggaccga tgaggctaaa gacgtagtgc acatccagtg cacgacagaa aaggacactg
     5821 caaatctgga tgtgcgctcc ggagcgctta gtacgctcgt cctcggtttg cgccaggcat
     5881 cagaggcttt catgagttcg ggcgaatcct caggtcaacc gctagaattg acaggtaccg
     5941 gccttgtttc ggtggaggac ggcagcatca tgctggagtt gatatttgat gggtcgatcc
     6001 gcgttatagc taacgt
//
LOCUS       DASTQY010000049        11718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1781982, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000049 DASTQY010000000
VERSION     DASTQY010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11718
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781982"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VFR82_01635"
     CDS             complement(<1..675)
                     /locus_tag="VFR82_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU4500329.1"
                     /translation="MTSHVFIDRPIMASVVSLVIVVMGLLSVQFLPVAQFPEITPPVV
                     QIDADYPGASAEVVAESVARAIEVQLPGIDNLVYFDSTSSNDGHVTIKLTFEIGTDPD
                     IAQVQTQNREKLAEPQLPPEVIRQGVSVKKMSPDLVGVIALKSTDPNHDAVFLSNYAI
                     LRLVDNVRRVKGVGDAMVFGQQNYSMRIILNPLMMARLSLTPTDIATIIREQNRDYPA
                     GLIGREP"
     gene            complement(679..1896)
                     /locus_tag="VFR82_01640"
     CDS             complement(679..1896)
                     /locus_tag="VFR82_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU4500330.1"
                     /translation="MLRAIDLTSAQAANRIRPLLTWALLVCLATASLAGCKQDAGSAP
                     APQMPQVEVITAATTTVPDEPEFIGQAEASRPVEIRSQVTGLIKAVLYQEGRDVKKGD
                     RLYQIDPVPFQAAAASAKAKIAQAEAKVVQAKQDLARVKPLLNEQAVSQKDVDDAVAE
                     DLSSKAALQGAKADLIKAQFDLDNTLITAPIDGLIERTRYYEGRLVSAQTDLLTMVHR
                     VDPMYVVVSIPESFILKRRRDIEEKRITHPGVYQLRGEVMLLDGSTYPHEGVLDLMEP
                     GLRTETGARVVRITISNPKRELLPGQFVKVKFKGDTKPNVVLIPQRAVLQGPQGPFVY
                     IVGPDEHVEMRPVQASIWQGDQWLIDQGIKAGDRVVVNGLMKIGPGAPVKAVPLGEAP
                     PPPASPSEQPKQG"
     gene            complement(1987..2265)
                     /locus_tag="VFR82_01645"
     CDS             complement(1987..2265)
                     /locus_tag="VFR82_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500331.1"
                     /translation="MLKITTNVSDDATRITLEGRLAGPWIGELERCWRESEQSAAGRQ
                     LVVDLTGVTFVEQEGKALLSRMCQSGADLLATGCCMRSIVEDAKKQIR"
     gene            complement(2302..5403)
                     /locus_tag="VFR82_01650"
     CDS             complement(2302..5403)
                     /locus_tag="VFR82_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU4500332.1"
                     /translation="MHMRSFTDVFIKHPVLAIVVNLVIVLAGWRALTTLPVQQYPKIE
                     SSSVVITTVYYGASAETVRGFLSTPVERVVSAIGGVDYVESTSRAGVSTVTVHLKLNH
                     SSTAALAEVTARLQQVRSELPAEAEPPVVEVQRADRPYASFYLSFSSSERTVPAVTDW
                     LLRTLQPQLSTLSGVQRVTIEGGRQIAMRIWIDPDRLAAFNLSPGDVQGALRRNNYLA
                     AVGRTKGNLVQVNLLANTDLRSTEEFEDLIVADRGGAIVRLKDVAKVEREAEEADMVA
                     KYNRTEGVYLGVWPVPGANEIDVAGRLRDEMERIRPTLPPDIDMRLVWDGTMFMRNAL
                     TEITKTLSETILIVALVVFLFMGSVRTALVPLVAMPVSLIGAALFMFAFGFSLNLLTL
                     LAIVLSVGLVVDDAIVVVENVERHVRQGKTRIEAALAGARELIGPIIAMTITLAAVYT
                     PIGFQGGLTGSLFLEFAITLAVAVVLSGVVAVTLSPVMSSRFVHPQGKEGRMAVLVNR
                     TFELVRSGYARLLDRALDMRWGIVAAAVLVMIAAWPLYLFSRQELAPVEDQSHISLFF
                     EASPDSTVAATNRQHLQIVDAVTAFPETQFTWSLTTAWGGFGGLVAKDWHERARSTEQ
                     MYGQVFGAVSQVPGLRVFPRLDPPLPTPGQYDVELVLQSDVPAQQMLETVGAVLGAGW
                     QSGKFLYVDTDLKIDLPEAHVVLDRERLADLGLDLAGVGRELGTLLGGAYVNRFNYFD
                     RSYKVIPQIGDKDRATVEPLLDLKIKTPAGQLVPVSTFTHIETSTGPRTLNRFQQRNA
                     VRIFGGVKPGVTKAEGLRILEAAAASSQGPRVVLDYAGESRQIRQEGGALTVTLGFAV
                     LLIYLVLAAQFQSFRDPLIVLLGSVPLAISGALVFSFLDLTTINIYSQVGLITLVGLI
                     AKNGILIVEFANQLQTSGLARVAAVREAALTRLRPVLMTSAATVFGHLPLVLASGPGA
                     AARNSIGMVLVTGMTVGTLFTLFVVPVFYSLIAAEHQPDAVGSAEVEMVEDDEALLAG
                     AKA"
     gene            complement(5429..6562)
                     /locus_tag="VFR82_01655"
     CDS             complement(5429..6562)
                     /locus_tag="VFR82_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU4500333.1"
                     /translation="MKRQRHWVGSVALPVIVLLMGVGLAAWKYDAIQAGQAASASQPE
                     PMETVTVALAKEIEHRQTTTSIGTVLALKSITLRNELAGTVRDVRLSPGQIVEPGMVL
                     VALDVSVEEAELKAQEAQAALAKTVLDRRQNLNHEMATTQEEVDRARADLDVARAQIA
                     RTKAIIARKTIRAPFRSRVGIADVHPGQYLNEGTLLTTLQGIDDAAHVDFTVAQQVAA
                     GLREGEVVEVFAGTPAPITAKIVALDARIDPATRNAVVRARIEGSGNAPSPGSSVRVR
                     IPVGASRKAVAVPVSALRKGPEGDQVFVIAQDKDGRARAHVQQVESGAMFGDEVVIHA
                     GLIAGEQVAASGSFKLREAALVAVAGAGAQNPQSEQRQAVSQH"
     gene            complement(6773..7759)
                     /locus_tag="VFR82_01660"
     CDS             complement(6773..7759)
                     /locus_tag="VFR82_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500334.1"
                     /translation="MELKMPTAEQAYWGLRAMKTVALADSVLDDTERQMLASVQTILG
                     TNYAVEDLAPVAPEELASALIDPQIRHQLTQGLIVVSLIDGKANVRETEAVEEFARAL
                     QVDAAEVRDLRYLINGEMLRLRLDLARRFWLREKVKDIWENERLKGLYKFVRGMLGRY
                     EDAPLASRYQALEHYPQGSLGRSYWEYCRANGFPLPGEKGGAAEQVLFHDCAHVLSGY
                     GTTPDEEVQVACFSAGFQRRDPWTFVFFVLLQFHVGIRLTPITAARTGFFDPVKAMIA
                     IRRGAAMTVDLNNGWDYWPVMGEQVEELRRRYNILPAEAFLPAARPLAASPA"
     gene            complement(7808..8761)
                     /locus_tag="VFR82_01665"
     CDS             complement(7808..8761)
                     /locus_tag="VFR82_01665"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEU4500335.1"
                     /translation="MNETLDFLTQHGALVVAAVVFAEQIGLPLPALPFLIAAGALAGT
                     GQMAAGVALVSAVLAAMAGDQVWFELGRRRGRQVLNWLCRISLEPTSCVRRTEDFFSR
                     HGVRALIVAKFVPGFSTIAPPLAGIVGLSVPQYLLFNGLGTLLWAGTGIGLGWIFSDQ
                     VEQALTISAQIGPTIGLTLIAAAVSYVGYKAAHRYRMGDGVPRMTVRQLTDKLAAGEQ
                     PVIVDLRSLAARQQEPGIPGAVSLALEEVVAHQHELPRDRDIILYCACPRDASSVEGV
                     LRLRKLGFTRVWPLAGGLGAWNAAANPPAVVQLSNRQIVPA"
     gene            complement(8774..9244)
                     /locus_tag="VFR82_01670"
     CDS             complement(8774..9244)
                     /locus_tag="VFR82_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500336.1"
                     /translation="MNRTSPTIDLFTLIGMMATIAGIFLCLFFLFTPTTFGAPATDVL
                     QDRSTDLYISMRWLQPTLGKAIVEDALIRQRDNDASDAVMLQPGVGSVQWVIGRVVVE
                     LHRSRLVAGLPGGHQEDDQRIVRIARHAAVQFQEHLTGEERVFERVTSQADVIF"
     gene            complement(9459..>11718)
                     /locus_tag="VFR82_01675"
     CDS             complement(9459..>11718)
                     /locus_tag="VFR82_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU4500337.1"
                     /translation="LRWPKVTQRMQQDGVNSFCVVPLTTAVRQLGAMGFASLRKHAYG
                     EGDLEFLQEVGKQVAVAVDNVLHHQDLLRDRDRLRLLLEVTESIASHRDLAELLRDLA
                     QRLPSIVPFDYINIVLHEPARQVMRLWLLVATVPSTISPGLETPVEESPGGWVWTHQE
                     PLTVNDVSQEHRFPRLMSMLRENGVKSFCAVPLTTAQRRLGAMGFGSLQLRTYRETDL
                     NFMQQVAKQVAVAVDNAFNSESTRAYQQQLARERDLQRLLLEVNNAVISHLELRDLLK
                     AVSVSLSRVVPHDLAGFALFDQDTQRLLAHALDFPSNQGFVETGHAIPLEGTPEGRAF
                     TSRQTVLIRNIDTAEFPADIMKRAKAEGLRSGCAVPLVSHGRVLGTLSVVSMREEAFS
                     EQDAELLTQIGAQVAIAVENALAYKQIAQLKDKLTEEKLYLEEEIQTEYNFQEIVGES
                     QTLRRVLKDVETVAKTSSTVLILGETGSGKELIARALHNLSDRRERTFVKLNCAAIPT
                     GLLESELFGHERGAFTGAIATKIGRFETADRGTLFLDEVGEIPLELQVKLLRVLQEQE
                     FERLGSTRTQRVDVRLVAATNRDLSHMVEEQKFRSDLYYRLKVFPVTVPPLRERAEDI
                     PALVRHFAQKFALRMNKRIETIPAEAMKALQAYPWPGNVRELENFIERAVILSAGADL
                     VVPLSELKVSIPPSNGSPKTLEDAEREHILKALRDAKWTIGGSAGAAAKLGMKRTTLQ
                     SKMQKLGIVRPG"
BASE COUNT         2413 a         3628 c         3358 g         2319 t
ORIGIN      
        1 cggttcccgg ccgatcagtc cagccggata gtcccggttc tgttcgcgga taatcgtggc
       61 gatgtccgta ggggtcagac tcaaccgggc catcatcagc ggattcagga tgatccgcat
      121 gctgtagttc tgctggccga aaaccatcgc gtctccgacc cctttgaccc ggcgcacgtt
      181 atccaccagt cgaagaatgg catagttgga gagaaatacc gcgtcatggt tgggatcagt
      241 cgatttcagg gcaatgaccc ccacgaggtc gggggacatc ttcttcacac tcaccccttg
      301 cctgatgact tctggcggca gttgcggctc tgcgagtttt tcacggttct gagtttggac
      361 ctgggcaata tcagggtcgg tgccgatttc aaaggtcagt ttaatagtga catgcccatc
      421 gttgctgctc gtactatcga agtaaacgag gttgtcgata cctggcaact ggacttcgat
      481 ggcgcgtgcc accgattcgg cgaccacttc ggcgctggcg ccaggataat ccgcgtcgat
      541 ctgaacgacg ggcggagtga tttcaggaaa ctgggcgacc ggcaggaact ggacggacag
      601 caggcccatg accacgatca caagggacac gaccgaggcc atgatgggcc gatcgatgaa
      661 gacgtgggag gtcacgcgtc acccctgctt cggctgctcg gaaggagacg ctgggggcgg
      721 cggggcttcg ccgagaggga cagctttgac cggcgcaccc ggaccgattt tcatcaatcc
      781 gttcactacc acgcggtcgc cggctttgat gccttgatcg atgagccatt gatcgccctg
      841 ccagatcgag gcctgaaccg gccgcatctc gacgtgttcg tcgggcccca cgatgtaaac
      901 aaacggccct tgcggacctt ggaggacggc acgttgcggg atcaacacga cgttgggttt
      961 agtgtctcct ttaaatttca ctttcacgaa ctgccctggc aagagctccc gctttggatt
     1021 ggaaatcgtg attcgcacga cacgcgcacc ggtttccgtg cggagacccg gttccatcag
     1081 atccagcacg ccttcatgcg gataggtgga gccgtccaag agcataactt cgcctcgaag
     1141 ctggtacacg ccgggatggg tgatcctttt ctcttcaata tcgcgccggc gcttcaatat
     1201 aaaactttcg ggaatgctca ccacgacata catgggatca acgcgatgga ccatggtcag
     1261 cagatcggtt tgtgccgata cgagccggcc ttcataatat cgggtgcgtt cgatcaaccc
     1321 atcgatgggg gcggtgatca gcgtattgtc caagtcgaac tgcgccttga tgaggtccgc
     1381 cttggcgcct tgcaaggccg ccttggagga aaggtcctca gccacggcgt cgtcgacgtc
     1441 cttctggctg accgcctgtt cgttcaatag cggtttgact cgcgccaggt cctgtttggc
     1501 ctgcacgact ttggcttccg cctgggcgat ctttgccttg gcactggccg ccgcggcttg
     1561 aaacggaacg ggatcaatct ggtacagacg atcgcccttt ttgacgtcgc ggccttcctg
     1621 atacaacacc gctttgatca agcccgtcac ttgcgatcga atctcgaccg gacgagaagc
     1681 ttccgcctgg ccgatgaatt ccggctcgtc aggaaccgtc gtggtcgccg ccgtgatcac
     1741 ttcaacctga ggcatctggg gggcaggggc agagcctgcg tcttgcttgc acccggcgag
     1801 gctcgctgtg gcaaggcaga cgagtaacgc ccaagtcagc agcgggcgta ttcgattggc
     1861 cgcttgcgcc gatgtcaggt cgatggctcg caacatagga tgtgtcctcg gcacccgtgt
     1921 ggcatcttag agggccgcag actttcttcg caagtgcggc gtgcgcgcgt tgctctttct
     1981 cttcacttac cggatctgtt ttttcgcatc ctcgacgatg gatctcatgc aacagcctgt
     2041 ggcgagcaaa tcggctcccg actgacacat tcgactcaag agcgcctttc cttcctgttc
     2101 aacaaacgtg acaccggtca aatcgacgac cagttgccgg ccggcagcgg attgctccga
     2161 ctcacgccag caacgttcga gttccccgat ccatggacct gccaggcgcc cctcgagcgt
     2221 gattctggtt gcatcgtcag atacgttagt ggtgatcttc aacatgatac gaaaccttct
     2281 ccttcattca tgcacatagc ctcaggcctt ggctccggca agcaatgcct catcatcctc
     2341 caccatctcc acctctgcgc ttcccaccgc atccggctga tgctcggcgg cgatcaacga
     2401 gtagaacacc ggaaccacga agagcgtgaa cagcgttccg accgtcatgc ctgtcaccag
     2461 caccatgccg atgctgttgc gcgccgcggc gccgggtcct gaggccagca caaggggaag
     2521 atgaccgaag accgtagccg ccgaggtcat caacaccggc cgcaagcgtg tcaaggccgc
     2581 ctcacgcacc gccgcgactc gtgcgagccc gctcgtttga agctgattgg caaattcaac
     2641 gatcaaaatc ccgttctttg caatgagtcc gacaagggtg atcagcccga cctgggagta
     2701 gatattgatg gtcgtcagat ccaggaaact gaagaccagc gcgccggata tcgcaagggg
     2761 aacagagccg agcaggacga tcagcggatc acggaagctt tgaaactggg ctgccagcac
     2821 cagataaatc aacagcacgg caaacccgag agtgaccgtg agagcaccgc cttcctgacg
     2881 gatctgtcgg gactcgcccg cgtagtcaag aacgacccgc ggtccctggg acgacgctgc
     2941 ggcagcttca aggatgcgga gaccttcagc tttcgtcacc cccggcttca cgccaccaaa
     3001 aatccgcacg gcgttgcgct gctggaagcg gttcagggtg cggggcccgg tgctcgtttc
     3061 gatgtgcgtg aaggtcgaca ccggcacgag ttgaccggcc ggcgttttga tcttgagatc
     3121 gagaagcggt tccaccgtcg cgcgatcttt atcaccgatt tgcggaatga ctttgtagct
     3181 gcggtcaaaa tagttgaagc ggttgacgta ggccccgccc aagagtgtgc ccagttctcg
     3241 gccgacgccc gcgaggtcga gccccaaatc agccagacgc tcgcggtcta ggacaacgtg
     3301 ggcctccggg agatcgattt ttaggtccgt atcaacgtac aggaatttcc cgctctgcca
     3361 tcccgcgccg aggaccgcac caaccgtctc tagcatctgc tgggccggta catcgctctg
     3421 caacaccagt tcgacatcgt actggcccgg cgtcggcagc ggcgggtcca gacggggaaa
     3481 cacgcgcaat cctggcacct gtgataccgc gccgaagacc tgaccgtaca tttgctcggt
     3541 cgagcgcgcc cgttcatgcc agtccttggc gaccaggccc ccaaatccgc cccacgccgt
     3601 cgtcaatgac cacgtgaatt gtgtttcggg aaaagccgtg acggcatcca caatctgcag
     3661 atgctggcga ttggtggccg ccaccgtaga atcgggcgat gcttcaaaaa acaagctgat
     3721 atggctttga tcctccaccg gcgcgagctc ctgtcgtgag aagagataga ggggccaggc
     3781 ggcgatcatg accagcaccg ccgcggctac aataccccag cgcatgtcca gcgcccgatc
     3841 gagcaagcga gcatacccgc tgcgcaccaa ctcgaacgtg cggttcacga gcacagccat
     3901 tcggccttcc ttcccttgcg ggtgcacgaa gcgtgaactc atgacgggcg aaagagtcac
     3961 ggcgacgacg cccgataaca cgaccgccac cgccagggtt atggcgaatt cgagaaacag
     4021 cgaaccggtc aaaccgccct gaaagccgat cggcgtatac accgcggcca gcgtgattgt
     4081 catcgcaatg atgggaccga tcagttctcg ggcgcctgcc aatgccgcct cgatccgggt
     4141 cttgccctga cgcacatgcc gctcgacgtt ctcaaccacc acgatcgcgt cgtcgacgac
     4201 gaggccgacg gacaggacaa tggcgagcaa ggtcaaaaga ttgagactga agccaaaggc
     4261 aaacataaag agcgcggctc cgatgagcga caccggcatg gcgacgagcg gcacgagcgc
     4321 cgtgcgcacg gaacccataa agagaaatac gaccagcgca acgatgagga tggtctccga
     4381 caatgtcttg gtaatttcgg tcagcgcgtt ccgcatgaac atcgtgccgt cccagacaag
     4441 ccgcatgtcg atatcggggg gcagcgtggg tctgatccgc tccatttcat ctcgcagccg
     4501 cccggcgaca tcgatttcgt tagcaccggg aaccggccag actccgagat acacgccttc
     4561 cgtccggttg tacttcgcaa ccatgtcggc ttcttcggct tcacgctcga ccttcgcgac
     4621 gtccttcagt cgcacgatgg ctcccccacg gtcagccaca atcagatcct cgaactcctc
     4681 agtcgaacgg aggtcggtat tggcgagcaa gttgacctgg acgagattgc ccttggttcg
     4741 accgacggcg gccaaatagt tgttccggcg aagcgctccc tgcacgtccc cgggcgatag
     4801 gttgaatgct gccaaccggt ctggatcgat ccaaatgcgc atggcaatct ggcgcccgcc
     4861 ttcgatcgtc acccgctgca cgcctgacag tgtggagagt tgcggctgca gggtgcgcag
     4921 cagccaatcc gtgacggcag gcactgtgcg ctcgctggag gaaaagctga gataaaagga
     4981 ggcgtaggga cgatccgccc gctgcacttc gacgaccggc ggctcagcct cagccggcaa
     5041 ttcagaccgc acttgttgaa gccgcgcagt gacctcggcc aaggccgccg tgctgctgtg
     5101 gttcaatttc aaatgcaccg tcaccgtgct cacaccggca cggctggttg actcgacata
     5161 gtccacgccg ccgatcgcgg aaacaactcg ctcgaccggc gtgctgagga acccacggac
     5221 tgtctcggcg ctcgcaccgt aatagaccgt cgtaatgacc accgacgagc tctcgatctt
     5281 cggatactgc tgcacgggca gagtcgtcag agcacgccaa ccggccagga caatgaccaa
     5341 gttgacgacg atcgccaaca ccggatgctt gatgaatacg tcggtgaatg aacgcatgtg
     5401 cataaatgac tctcgttcct cgaaaatctc agtgttgact caccgcttgc cgctgttccg
     5461 actgaggatt ctgggcgccg gcgccggcaa ccgcgacaag agctgcctca cgaagcttga
     5521 atgatccgga agccgcgacc tgctcgccgg cgatgagtcc tgcatgaatg accacctcgt
     5581 cgccgaacat cgcgccgctc tcgacctgct ggacatgggc acgagcacga ccatccttat
     5641 cctgcgcaat cacgaacacc tgatcccctt cggggccctt gcgcaatgca ctgaccggaa
     5701 cggcaaccgc tttgcgcgaa gccccgacgg gaatacggac gcgaaccgag gacccaggcg
     5761 atggcgcgtt gccggaacct tcgatccgcg ctcggaccac ggcattgcgt gtcgccgggt
     5821 caatgcgcgc gtccagcgcg acgattttag cggtgatggg agcgggggta ccggcaaata
     5881 cctcaacgac ttcgccttcc cgtaggccgg cggcaacctg ctgtgccaca gtaaagtcca
     5941 catgcgcggc gtcatcgata ccctgaagtg tcgtcaagag ggttccttca ttgagatact
     6001 ggccaggatg aacgtctgcg ataccgaccc gtgaacgaaa cggcgcgcga atcgtcttgc
     6061 gggcaatgat cgctttggtg cgggcaattt gcgctcgggc gacatccagg tctgcgcgcg
     6121 cccggtccac ttcttcctgc gtcgtggcca tttcatggtt cagattctgc ctacgatcga
     6181 gcaccgtctt ggccagagca gcctgcgctt cctgggcctt cagctcagct tcttccaccg
     6241 acacatcgag cgccacgagc accatgcccg gttcaacgat ctgccccggt gacaaccgca
     6301 cgtcgcgcac cgtgccggca agctcattcc gtagcgtgat tgacttcaac gccaggaccg
     6361 tgccgatcga ggttgtggtt tgtcggtgct cgatctcctt cgcgagggcg acggtcaccg
     6421 tctccattgg ctcaggttga ctcgccgaag ctgcctgacc ggcttgaatg gcatcatatt
     6481 tccaagctgc caggccaacg cccatcagaa gaacgatgac cggcagtgcg actgaaccga
     6541 cccagtgacg ctgacgcttc atgaagcacc tccacgcttg cctgagaaac caaccgtcta
     6601 ttcactactg agaggagaca gcaaccttcg ttccacctct cgagcaatcg tctgccacat
     6661 aagtagctga agttgcaaag aacttttggc atctgcttcg aagagagctc gatgtgccga
     6721 acaccattcg ccgcctaatc ggcaggtgcc gaagcatcgg catctcccac gctcacgccg
     6781 ggcttgcagc cagcggtcgg gcagccggta gaaaggcctc tgccggcaga atgttgtagc
     6841 gcctcctgag ctcctcgacc tgctcgccca taacaggcca atagtcccat ccattgttca
     6901 ggtcgacggt catcgccgca cctcgccgga tggcgatcat cgccttcacc ggatcgaaaa
     6961 atccggttct cgccgcggtg atgggggtga ggcggatgcc tacatggaat tgcagcagca
     7021 caaaaaatac gaacgtccac ggatcccgcc gctggaaccc tgcgctgaaa caggccactt
     7081 gcacttcttc atcgggcgtc gtcccatacc cggacaagac atgcgcgcaa tcatggaata
     7141 gaacctgttc tgccgctccg cccttctcac cgggcaacgg aaagccattc gcccggcaat
     7201 actcccaata ggaccgtcca agggatccct gaggataatg ctccaaggct tgataccgag
     7261 acgcgagcgg ggcgtcttcg taacgtccca gcatgccgcg cacaaatttg tagagtcctt
     7321 taagacgctc gttttcccaa atgtccttca ccttctccct cagccagaac cggcgggcca
     7381 gatccagacg aaggcggagc atctcaccgt tgatgaggta tcggaggtcc cgaacttccg
     7441 ctgcgtccac ttgcaacgcc cgggcaaatt cctcgaccgc ctctgtttcg cgcacgttgg
     7501 ccttcccgtc gatcagcgag accacgatca acccttgtgt gagttgatgt ctgatctgtg
     7561 ggtcgatcaa agcggaagcc agctcctccg gcgcgacggg cgcaaggtcc tcgactgcat
     7621 agttggttcc gagaatcgtc tgcaccgatg ccagcatctg ccgctccgtg tcgtcgagga
     7681 cgctatctgc caaggcgacg gttttcatcg cacgcagccc ccaataagct tgttccgccg
     7741 tgggcatttt cagttccatc ttctttctcc tctgaataac gattgtgaag tcggctccgg
     7801 ctgatgttca tgccgggacg atctggcgat tggatagctg cacgaccgca ggcggattgg
     7861 ccgccgcatt ccaggctccg agacctccgg caagcggcca tacgcgcgtg aatcccagtt
     7921 tgcgaagtct caaaacaccc tcgacgctcg aggcgtccct cgggcaggcg caatacaaaa
     7981 tgatatcgcg gtctcgcggt agctcgtgct ggtgagcgac cacctcctcg agcgccagag
     8041 acacggcacc cggaattccc ggctcttgct ggcgcgcggc aagcgaccgc agatcgacaa
     8101 tcaccggttg ttcgccggcg gcgagcttgt ccgtcagttg ccggacggtc attcgtggaa
     8161 cgccatcgcc catgcgatac cgatgagcgg ctttgtagcc gacatagctc acggccgccg
     8221 cgatcagcgt cagaccgatg gtcggcccaa tctgagcgct gatcgtcaac gcctgctcca
     8281 cctgatcact gaaaatccat ccgagaccaa ttcctgttcc agcccagagc aaggttccca
     8341 atccattgaa gagaaggtac tgtggcaccg agagacccac gatcccggcc agtggcgggg
     8401 caatcgtgct gaagcccggc acgaacttgg cgacgatcaa cgcgcggaca ccatgacggg
     8461 aaaagaaatc ctccgtccgg cggacgcagc tagtcggttc caaggaaatg cggcatagcc
     8521 agttcaacac ctgccggcca cgccgacgac cgagctcgaa ccacacctga tcacccgcca
     8581 tcgcagcgag gaccgccgag acaagcgcca cgccagccgc catttgtccg gtcccagcca
     8641 atgcgcccgc ggcaatcaag aagggcaggg caggcagggg aagtccaatc tgttcggcaa
     8701 agaccacggc agccacaact agcgcgccat gctgagtcag aaaatctaac gtttcgttca
     8761 tgatggactc cgattaaaag atcacgtcgg cctggctcgt cacacgctcg aacactcgct
     8821 cttcgccagt gaggtgttcc tgaaactgta cggccgcgtg ccgcgcaatc ctgacaatcc
     8881 gctgatcgtc ttcttgatgg ccgccgggca gtcccgcaac cagccgcgat cggtgtaatt
     8941 ccaccacgac gcggccgatg acccattgga cacttccaac ccccggttgg agcatcacgg
     9001 catccgaggc gtcgttgtct cgctggcgaa tcagcgcatc ttccacgatg gccttaccga
     9061 gagtcggctg cagccaacgc atagagatgt agagatccgt cgaacggtcc tgtaagacgt
     9121 cggtcgccgg ggcgccaaag gtggtcggcg tgaaaaggaa aaacagacac aggaatatgc
     9181 cggcgatcgt ggccatcatg ccgatcaacg tgaacagatc tatggttggg gatgtccggt
     9241 tcatgggtcc tccgatcagc tcctgtgcac tgctcatgcg gcgctgtgtc agcaaggacc
     9301 attccgtcgt gcaccgagcg caccccgtac atcgcgtaga tgcaaccctg cgatcttccg
     9361 tgggaaacga gtcgttgatg gggtgtgagc tgcggagcag agaagaggag agccggaaaa
     9421 acgtgccgac atctcggcgc gatgccgacg cgtcggcgtt atcctggacg gacaataccg
     9481 agtttctgca ttttcgattg cagggtagtg cgcttcatgc ccaatttggc cgctgcaccg
     9541 gcagaacctc caatcgtcca tttagcgtcg cgcagcgcct tcagaatatg ctctcgctcg
     9601 gcatcctcga gtgtcttggg cgaaccgttc gaaggaggga tcgatacttt taattctgac
     9661 agtgggacga cgagatcggc ccccgccgat aagatgactg cccgctcgat gaagttttcc
     9721 agctcacgca cattgcccgg ccaggggtag gcctgcaggg ccttcatggc ctcggcggga
     9781 atcgtttcga tccgcttgtt catccgaagc gcaaacttct gggcgaagtg gcggaccaac
     9841 gcggggatat cttcagcccg ctcgcgcagc ggcgggacgg tgaccggaaa gactttgagc
     9901 cggtaataga gatcgctgcg aaacttttgc tcctcgacca tgtgactgag atcgcgattc
     9961 gtggcggcga cgagccggac gtccacgcgc tgggtcctgg tactgccaag ccgctcgaat
    10021 tcctgctctt gcaagacgcg aagcagcttg acttggagct ccagcggaat ctctcccacc
    10081 tcgtccagaa acagcgtccc tcggtctgcg gtttcgaacc ggccgatctt ggtcgcgatt
    10141 gcgcccgtga acgctcctcg ttcatgtcca aagagttcgc tttcgagcag acccgttgga
    10201 atggccgcac aattgagctt gacgaaggtg cgttcgcgcc gatcactgag attgtgcagg
    10261 gccctggcga ttaattcctt tccgcttccg gtttctccga gaatcaggac agtcgaggac
    10321 gtcttcgcga ccgtttccac gtccttcagg acgcggcgaa gggtctggct ttcaccgacg
    10381 atttcctgga agttatattc ggtctgaatt tcctcttcga ggtagagctt ctcttcggtc
    10441 aacttgtctt tgagctgagc gatttgtttg taggccaggg cattttcaac ggcgatcgcc
    10501 acctgcgcgc cgatttgggt gaggagttcc gcatcttgct cactaaaggc ttcctcacgc
    10561 atgctgacaa cgctcagtgt gccgagcact ctgccgtgag acaccagcgg gactgcacat
    10621 ccggatctca acccttcggc tttcgcccgc ttcataatgt cggctgggaa ctcggctgta
    10681 tcgatatttc ggataaggac ggtctgtcgg gacgtgaacg cccgaccttc cggcgtgccc
    10741 tccaggggaa tcgcatgacc ggtttcgaca aagccctggt tgctcggaaa atccagggcg
    10801 tgcgcgagca gccgttgtgt gtcctgatcg aacagcgcga atcctgccag atcgtgggga
    10861 accacgcggc tgaggctgac agaaacggcc ttgaggagat cgcgcaattc aagatgcgaa
    10921 atcacggcgt tgttcacttc gagcaagaga cgctggaggt ctcgttcgcg tgccaattgc
    10981 tgttgatacg ctctggtact ctcggaattg aacgcgttgt ccacggcgac cgccacttgc
    11041 tttgcgacct gttgcatgaa gttgaggtca gtctctcgat aagtcctcag ctgtaaactt
    11101 ccaaacccca tggccccgag ccggcgctgc gccgtcgtca gcgggactgc gcagaaggac
    11161 ttgacgccgt tctcacgcaa catcgacatc agtctgggaa aacggtgttc ctgggacacg
    11221 tcgttgacgg tcagaggctc ttgatgggtc cagacccatc cgcccggtga ctcttcgacc
    11281 ggcgtttcca ggccggggct gatcgtgctg ggcacagtgg cgaccagcag ccagagacgc
    11341 atgacctgac gggccggctc gtgcaataca atattgatat agtcgaatgg aacgattgaa
    11401 ggcaggcgtt gtgcgaggtc tcgcaacaac tcggccagat cgcggtgcga agcaatggat
    11461 tccgtgacct ccagaagcag gcgcagtcga tcccgatcgc ggaggaggtc ctgatgatga
    11521 aggacgttgt ccactgccac ggcaacctgc tttcccacct cttgaagaaa ctccaagtcc
    11581 ccctctccgt acgcgtgttt gcggaggctt gcaaagccca tcgcccccaa ttggcggacg
    11641 gccgtcgtca gcggcacgac gcaaaacgaa ttgacaccat cttgctgcat ccgttgcgtc
    11701 accttcggcc agcggagc
//
LOCUS       DASTQY010000050         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_769790, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000050 DASTQY010000000
VERSION     DASTQY010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769790"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..356
                     /locus_tag="VFR82_01680"
     CDS             168..356
                     /locus_tag="VFR82_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500338.1"
                     /translation="MESPELRKFCIAIQNLREAMTHDLPLDEFDQICLENYIALLQIT
                     YMEWKRRNIQQPPYRRAA"
     gene            986..2512
                     /locus_tag="VFR82_01685"
     CDS             986..2512
                     /locus_tag="VFR82_01685"
                     /inference="COORDINATES: protein motif:HMM:NF021619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4500339.1"
                     /translation="MRVSNALIELSPSRKAFLPRRMQGRGYAYGAVILVLTLGIRMLL
                     AQRKQAEAQLKAIAWLLREGRNPFTVPEPQPYGDLSELNTERTILRTVGKDLMVNVAG
                     DALHLIGTSGAVCERNGDYASCLVASDWCRFLDSTSRERCATTDNGAALASGEWHCRN
                     SCRDISARSMDTGEPVDLPCNGGIHIYAAPIKVDGETVGSISIGYGDPPKDEAQLIEV
                     AERYGVRADQLRELSAGMPSRPQFIVAVAKARLRSAALLLGEIIKRKRAEEALADLTK
                     NLEARITERTEQLRALATELNLAEQQERKRLATELHDHLQHLLVLAKLKVGQGKRHAE
                     PIPALIELLKQLDEALSQALRYTRTLVAELSPPVLRDYGLAAALKWLSEYMQRLSMTV
                     MVHVPESSELTLPEDQAVLLFQSVRELLINCYKYAGTSRATVLLEQREGQLRIQVRDE
                     GVGFDPTAATPAESPAGGPSSKFGLFSIRERMTALGGSFEMRSAPGQGTTATLILPAS
                     "
     gene            2719..3138
                     /locus_tag="VFR82_01690"
     CDS             2719..3138
                     /locus_tag="VFR82_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4500340.1"
                     /translation="MTPLSVQSSPPSNTIVLIEDNEEELQYWSKTLRSAPFNYTVLES
                     SDAQTGLELCRDRAIDCVLLDLDMPESGFHVLLELIPNRSHPKIPVIILTHLPHPNLW
                     EMAKHNGAQACLLKQRTSAQELHCAIQTAITSVKSQN"
     gene            complement(3135..>3385)
                     /locus_tag="VFR82_01695"
     CDS             complement(3135..>3385)
                     /locus_tag="VFR82_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500341.1"
                     /translation="EGYQFSVSWPNQPARSVENFSSANHVARGSVTLVGKKVDWRWAG
                     ARQVTKMETTVKLEAIERSQPFRRKGPTARASGHCLMS"
BASE COUNT          781 a          957 c          929 g          718 t
ORIGIN      
        1 accaggcgcc aacatgctga gcgacgcccg attcgtcgct tctcaagcaa cccgttgccg
       61 ggcctggtgg aacccctgtg ggcacaatcg tacaaaaaac ctgacgaatc atgttgctat
      121 gacaacaaac atgatgagta aaataatatg tcatgaaaga gacccccatg gaatcgccgg
      181 aactgagaaa gttttgtatt gccatccaga accttcgtga agcgatgaca catgatctgc
      241 cgcttgatga atttgaccaa atttgcctcg aaaactacat agctctcctc cagatcacgt
      301 acatggagtg gaagcggcgc aacatccaac agcctcccta ccgacgcgca gcgtaacgct
      361 ggccggcatt ccccgtttct gcaagaagac aacatactct ttagacgcgt ggcggcttac
      421 cgtgatgctg aagtatgggg cgctcgactc catctgccca agcgcacgag gaaggcattg
      481 ggtaaacaag gagccaagaa cgtcagccgg aaacgtcgtt ctttctgaca ggacgaaggc
      541 accttttggc ggcagccgtc atgacgcagc agagctctcc gcaatttctt ttcgccgtat
      601 acgtggcaga agtttctttt agaggagctg agcaggaacg cagatcacct tctgatcggc
      661 attaggcgtt cactttcgtc tacttgatcg ccgcctgggc cgttcctacg cgcgcaggaa
      721 accgtcgcgc caattagact agactccgta cggagatacc gcaatgaccg cagcaaccat
      781 aagacaaaat ccgtcagact aaatccctat cggtttcgta ttctctcacc tacacccctt
      841 acaacagtca cttactctca aggcgttcat ccacctaaaa tcagcaactt ctgagcgtgc
      901 tacaggccga tgctaggcat gaggattgcc gagtcctttg tcgggcacat gcgcctgctg
      961 agttttgcgg atatgagtta ttgccatgcg tgtaagcaat gccctcatag agctctcccc
     1021 ttcgagaaag gccttcttgc caaggcgtat gcaagggcgg ggatatgcct acggcgcggt
     1081 catccttgtt ctgacgcttg gcatccgtat gttgcttgcg caacggaaac aagcggaggc
     1141 tcagctcaag gcgatcgcct ggctcctgcg tgaaggccga aatcctttta ccgtgccgga
     1201 accgcaaccg tacggagact tatcggagct caacaccgag cggaccatcc ttcgtacggt
     1261 cggaaaagat ctcatggtca atgtcgccgg tgacgcgttg cacctcatcg ggacgtcagg
     1321 ggctgtgtgt gaaaggaacg gagactatgc ctcatgcctc gtcgcatccg attggtgccg
     1381 gtttctcgac agcacgtcgc gagagcggtg cgccactaca gacaacgggg ctgctctggc
     1441 gagcggcgaa tggcattgcc gcaattcatg ccgcgacata tccgctcgct cgatggatac
     1501 cggcgaaccg gtcgatctgc cgtgcaatgg cgggatccat atctacgccg ctccgatcaa
     1561 ggttgacgga gaaaccgtcg gaagcatcag cattgggtac ggggatccgc cgaaggacga
     1621 ggcgcagttg attgaggtag ccgaacgtta cggagttcgg gctgatcagc ttcgtgaact
     1681 cagtgccggc atgccgtctc gcccgcaatt catcgtggcg gttgccaagg cgcggttgcg
     1741 gagcgctgcc cttctcctgg gcgaaattat caagcggaaa cgcgctgaag aagccttggc
     1801 tgatttgacg aagaacctcg aagcacgaat caccgagcgc acggagcaac tccgggcctt
     1861 ggcaacggaa ctgaaccttg ccgagcagca ggagcgcaag cgactggcca ctgaactgca
     1921 cgatcacctg cagcatctcc tggtgctggc caagttgaag gtcggtcaag ggaagcggca
     1981 tgccgagccg atcccggcgc tcatcgagct cttgaaacag ctcgacgaag ccttatccca
     2041 ggccctccgg tatacgcgga ctttggtcgc agaattaagt cctcccgtgc tcagggacta
     2101 cgggctcgcg gcggcgttga aatggctgag cgagtacatg cagagactgt ccatgacggt
     2161 gatggtgcac gtaccggaat caagcgagct cacattgccg gaggaccaag cggtgctgct
     2221 gtttcagtct gtgcgagaac tcctgattaa ttgctataaa tatgcgggta cctcacgcgc
     2281 tactgtccta ctcgaacagc gcgaagggca gttaagaata caggtccgtg atgagggggt
     2341 gggcttcgat ccgacggccg ccacgcccgc cgagagtccc gccggaggtc cgtcatccaa
     2401 gtttgggctc tttagtattc gcgagcgcat gacggcgttg ggtggatcgt tcgagatgcg
     2461 atcagctcca ggacagggca ccacggcgac actgattctg ccggcaagtt agcgaatttg
     2521 ttgtcctgtc tgacgaatgc gcggctgtga gagacagcct gccgaaccgc ttggccgcga
     2581 aagaagactg tcagggggcc tgcctcatta gatagaacgg atcaatggcg cagaagtagt
     2641 tacgatgcct cttcctcact tgacaagagg tctatgagtt gcaaaaatca gtgcccctaa
     2701 agaaaggtga attggcagat gactccgctc tcagttcagt cgagccctcc gtccaatacc
     2761 attgtgctca ttgaggacaa cgaagaagag ttgcagtact ggtccaaaac tctacggagt
     2821 gcccctttca actataccgt gcttgaatcg tccgatgcgc aaaccggtct tgagctgtgc
     2881 cgagaccgcg ccattgattg tgtgctgctt gacttggata tgcctgaatc aggtttccat
     2941 gtcctgctgg aattgattcc gaaccggagt caccctaaga tccctgtcat catcctgacc
     3001 catctgccgc accccaacct ctgggaaatg gccaagcata atggagcgca ggcgtgcctg
     3061 ctcaaacaac gtacgtcggc tcaagagctg cattgtgcca ttcagacggc gatcacttcc
     3121 gtcaaatcgc agaactaaga cattaggcaa tgaccggagg cccgagccgt agggcctttt
     3181 ctacgaaacg gctgacttcg ttcaatcgcc tctagcttca ccgtggtttc catcttggtc
     3241 acctgtcgtg cgcccgccca tcgccaatcc actttcttgc cgaccaaggt cactgagccc
     3301 cttgcgacat ggttggcaga tgaaaagttt tcgacagaac gagccggttg attcggccag
     3361 gacaccgaga actgatatcc ctctc
//
LOCUS       DASTQY010000051         3456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_169525, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000051 DASTQY010000000
VERSION     DASTQY010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169525"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFR82_01700"
     CDS             complement(<1..206)
                     /locus_tag="VFR82_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500342.1"
                     /translation="MAARRKLATEHHSRLVKAWELVVNRAVVLNGNASSLARIASFGG
                     IGSIWAIRTLAEDDHSKFETLSSKV"
     gene            247..711
                     /locus_tag="VFR82_01705"
     CDS             247..711
                     /locus_tag="VFR82_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HEU4500343.1"
                     /translation="MPDLYESDFYAWMQQQAEAVCTGAWDEIDRDHLAEEIESLLRSD
                     AHRLWQHLRELMVWLLAWAYAPEQRDAHPHWHVRIVNERCEIEVIVDVWSNLAANVER
                     VLPKAYAQGREVAAEETGLPLETFPEAYLWTPEQVLKAPLEGPDSPFFGESR"
     gene            708..1118
                     /locus_tag="VFR82_01710"
     CDS             708..1118
                     /locus_tag="VFR82_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500344.1"
                     /translation="MMSQVSVHVTLTNARELVMSQLGRHALKSVHTCEVEALIDTERF
                     YSIVPKSIARRLGLARLRQSAMQPDGRWGEGEVTEAVQVDLYDRPATIDALVMGKCVQ
                     LSASVLGQTDLRYDKASGELRPNIGTWKRPVFRV"
     gene            1128..1769
                     /locus_tag="VFR82_01715"
     CDS             1128..1769
                     /locus_tag="VFR82_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500345.1"
                     /translation="MLTDEIKQKVEAATVKIIMEDDCFGSGVLIPGRMILTATHCVQH
                     TADGGMAIGDYYIETLETSQGFLKAGPIMVECMSDLAVLGGLDGQEFWDELIAFDEFC
                     ETVEPVGIHLKEVHPCMPFPAHLRTCEGEWMSGEATIFNVVSPIVAVAFSHAIPAGTS
                     GSPIVNDDGQIVGIVSNNSENPNRIQSSGSHPFPAMVLPWWILKRIRTGRSFS"
     gene            complement(1885..2070)
                     /locus_tag="VFR82_01720"
     CDS             complement(1885..2070)
                     /locus_tag="VFR82_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500346.1"
                     /translation="MRRGNIPQVMVALRNTTIGVLRWAGYRKIAKAGRSVSTACAKSL
                     MACLGSPSQDSMASDAP"
     gene            2205..2663
                     /locus_tag="VFR82_01725"
     CDS             2205..2663
                     /locus_tag="VFR82_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEU4500347.1"
                     /translation="MTPEEIIEDFQSFSDWEDRFCYLLDLADELPDMDEADKTDENRL
                     FGCQSRVWLKSELLPTDPPVLHFTGDSDAQLVRGLVAIVINVCNGRTSQQILDFDIAG
                     FFKALELERQLTRTRATGLASMVKRIKEIASAYEVMESSRFRRNFASPLV"
     gene            complement(2834..3097)
                     /locus_tag="VFR82_01730"
     CDS             complement(2834..3097)
                     /locus_tag="VFR82_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500348.1"
                     /translation="MRNETVPTEASRQYATAYAAHYTGRDLPVALQLYKKLMASHPTA
                     QEAGYSRTQVQNIVNAVVPKQELLDAQMELVLVHFERKRLIGE"
BASE COUNT          782 a          916 c          983 g          775 t
ORIGIN      
        1 actttagatg agagcgtttc aaatttggaa tgatcgtctt ccgcaagcgt ccgtattgcc
       61 cagatgctgc cgataccccc gaacgaggcg atcctggcca agctcgaagc gttgccgttc
      121 agcaccacgg cgcgattaac gacgagttcc cacgccttga ccaggcgcga gtgatgttcc
      181 gtcgccagct tgcgacgagc cgccacgaac gctggcgaaa gccacgaagg cggcaaaggg
      241 gtaggtatgc cggacctcta cgagagcgat ttttacgctt ggatgcagca gcaagccgag
      301 gccgtttgca ccggcgcctg ggacgagatc gaccgcgacc atctggcgga agagatcgag
      361 agcctacttc gcagcgacgc ccatcggcta tggcaacacc tacgggagct gatggtgtgg
      421 ctgctggcct gggcctacgc cccggagcag agggatgcac atccgcattg gcatgtgcgg
      481 atcgtcaacg aacggtgcga gatcgaggtg atcgtggacg tctggtccaa tttggccgct
      541 aacgtcgaaa gggtactgcc caaggcgtat gctcagggcc gcgaggtagc ggcagaggag
      601 acgggactgc cgctggagac ctttcccgag gcgtacctgt ggacaccgga acaagtgctc
      661 aaagcgccac ttgaagggcc agacagcccg ttttttgggg agtctcgatg atgagtcaag
      721 ttagcgtcca cgtcacgctc accaacgccc gcgagctcgt catgtcgcag ctaggccggc
      781 atgccctcaa gtcggtgcat acgtgcgaag ttgaagcgct gatcgatacg gagcgtttct
      841 acagcatcgt gcccaagtcc atcgcccgcc gtttgggcct cgcccggtta cggcaaagtg
      901 ccatgcagcc cgatggtcgc tggggcgagg gagaagttac ggaagcggta caggtcgacc
      961 tatacgaccg gccggcgacc atcgatgccc tggtgatggg caagtgtgtg cagctcagcg
     1021 cctctgtcct gggccagacc gacctgcgtt atgacaaggc aagcggcgag ctgcggccca
     1081 atataggcac gtggaagcgg cccgtctttc gggtttgagg tgtcgtcatg ttaactgatg
     1141 agatcaaaca aaaagtcgag gcagccaccg tcaaaatcat tatggaagac gactgctttg
     1201 gctccggtgt cttgatccca ggtcgcatga tcctgacggc cacgcactgt gttcaacaca
     1261 cggcagacgg tggtatggca ataggggatt actacatcga aactctggag acttcacagg
     1321 gctttctcaa ggctggtccg atcatggtgg agtgcatgtc ggatctcgca gtacttggtg
     1381 gacttgacgg acaagagttc tgggatgagt taattgcatt tgatgaattt tgcgagaccg
     1441 tagaacctgt cggtatccat ttgaaagagg tgcatccgtg catgccgttt ccggctcatc
     1501 tgcggacgtg tgaaggcgag tggatgtcag gcgaagccac cattttcaat gtggtatcgc
     1561 caattgtagc ggtagccttt tcccatgcca taccagctgg gacttcgggt tctccgatcg
     1621 tcaacgatga cgggcaaatc gtgggcattg tgagcaacaa tagcgagaac cctaatcgga
     1681 ttcaatcgag tgggtcccac ccgtttccag ccatggtatt accatggtgg atattaaaga
     1741 gaatacgcac gggccgatct ttctcataaa acgttccctt ttaaaccaaa ggtgacaatg
     1801 gacatcatgc ggaccatcgc cattgccaat cagaaagtcg ggagcggcaa gacaacaacc
     1861 tttgtcaacc tcgcggctag caaatcatgg ggcatcagac gccatgctgt cctggctcgg
     1921 actacccagg caggccatta aagacttcgc gcaggctgtg ctcacgctgc gcccggcttt
     1981 ggcgatcttg cggtaacccg cccaccgcaa caccccaatg gtggtattgc gaagcgccac
     2041 catcacttgc ggaatattgc cgcgacgcac ctgcgagcga tcttcatcga atgtgatatc
     2101 gcgtacccaa tgcgagcggt tcttaatcgc tcactgtcct ctgaccagct ccggtgaaag
     2161 cagggatgag cggaagcccc ggacgaatga ctaggatatg acatatgaca ccagaagaga
     2221 ttatcgaaga ttttcaatcg ttttcagact gggaggaccg gttttgctat cttctggatt
     2281 tggcggacga actgcctgac atggacgagg cggacaagac agatgaaaac cggctttttg
     2341 gttgtcaaag tcgagtttgg ctgaaaagcg aattgctgcc caccgacccg ccggtgctgc
     2401 attttactgg cgacagcgat gcccaattgg tgaggggatt ggtggccatc gtcatcaacg
     2461 tctgcaatgg tcgtacctct caacagatac tggatttcga cattgccggc ttttttaaag
     2521 ctctcgagct cgagcggcag ctcacgcgta cccgcgccac tggattggct tctatggtca
     2581 agcggattaa agagatcgca agtgcgtatg aagtgatgga atcgagtcgg ttccgtcgca
     2641 attttgcctc gccactcgtt tgaggtatga tcgcccttta cgagcaacgg tcattcatca
     2701 gggagcgaga cggtggcaat cagtttcaaa ggtgcgcatt ttcccaaaga gatcattctc
     2761 atgggttcga tggtatctgg cgtacccgct cagcacgcgc cgctgaccct caatcacgaa
     2821 aagtggacat gaactattct cctattagcc tcttgcgctc aaagtggaca agtacgagtt
     2881 ccatttgggc atccagcagt tcctgctttg gaactacggc gttgacgata ttttgaacct
     2941 gtgtcctgga gtaacctgct tcctgagcgg ttgggtgtga tgccatgagc ttcttgtaaa
     3001 gctggagagc cacaggcaaa tcgcgcccgg tatagtgtgc ggcgtacgcc gtcgcatact
     3061 gccgactcgc ctctgtaggt acagtttcgt ttctcatgtc cgttgctcct tctagaaaaa
     3121 agacgggtgg agaaaaagtg attcgctctt gctaagttgc tcgcttatcg tagctcagag
     3181 catcccagcg agagtgccaa gatctgaggg gaatggtaga cgtcgtgtga gcagtaccct
     3241 ttctcagaga taggaaagcg ttctgttcgg gcgcttagag cccggaagct gtcttcataa
     3301 attataggtt tcactccctc ccctgctttt aagcactctc cttgttcaac gatctctcaa
     3361 tccgaggtcc ttatgagcac gatggagtgt gccacatgac atgggacaca tatcccacga
     3421 aattactgac tctgaataag cgcaccaatc ccctca
//
LOCUS       DASTQY010000052         6451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1376851, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000052 DASTQY010000000
VERSION     DASTQY010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376851"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..508
                     /locus_tag="VFR82_01735"
     CDS             122..508
                     /locus_tag="VFR82_01735"
                     /inference="COORDINATES: protein motif:HMM:NF020534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098609 - cell-cell adhesion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin domain-containing protein"
                     /protein_id="HEU4500349.1"
                     /translation="MLSLRLGLPMIAAIALSAVPAFAADLETQVIDKNCYIEVFDDDD
                     FDMDDAHYKIQGPKEFASLKDLGGKNWNNDIESVIVGSNAKVKAYSDKDFKGTELAFA
                     PGQRVPNLGKMNMKDDIESMKIACGS"
     gene            676..3162
                     /locus_tag="VFR82_01740"
     CDS             676..3162
                     /locus_tag="VFR82_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4500350.1"
                     /translation="MPRKKPSPAAARSADGSAKNEDFERLGVFYLGRPYDPVIKQRKP
                     GWILYDSKDLVTHAVCVGMTGSGKTGLCLSLIEEAAIDNVPAILIDPKGDLSNLLLTF
                     PGLTAEEFRPWINEDEARKKGLPPDEYAAQQADHWKKGLASWQEDGARIKRFQDSADA
                     VIYTPGSHAGLPVSILKSFAAPPDDVRNDDELLRERISTTVTGLLGLIGVEADPIQSR
                     EHILISTILNEKWRRKEDLDLAALIHAIQSPPAGKIGVMDVDAFFPSKDRFALSMKLN
                     NLLAAPGFQAWLSGASLDVQELLYTPTGKPRLAIFSIAHLSDAERMFFVTLLLSQVVG
                     WMRGQPGTTSLRALLYMDEIFGYFPPVANPPSKLPLMTLLKQARAFGLGVVLATQNPV
                     DLDYKGLANTGTWFIGRLQTERDKARVLEGLEGASVSAGKTFDKSPMEQTLAGLGARI
                     FLMHNVHEDEPVVFETRWSLSYLRGPLTRSQIKTLMDPRRTESHTSKAASQAAGSARQ
                     STLESRPLVDPDVAQYVVPLRGRRPDDSRLVYVPMVLGAGQVRFADSKNGIDTTRDFH
                     IVVPVTDGAVVLDWDQGAATTITPADLEQTPADEAQFLPVPSSANKAKSYDVWRKEFA
                     GWLFRTQKLELLKSQASNEVSRPGESERDFRVRLQQGGRERRDEQIELLRRKYAPKIA
                     ALQERVRRADQQREKQEVESRSSQVQAAISVGASILGAFLGRKSISATNIGRATTAIR
                     GAGRVLKESRDVNMAEENVAALRQQLADLEAQFGSERDVITAATDPLQEKLESISLKP
                     TKTNITVKLVALTWTPHWRSAAGTLTPAWE"
     gene            complement(3177..5930)
                     /locus_tag="VFR82_01745"
     CDS             complement(3177..5930)
                     /locus_tag="VFR82_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU4500351.1"
                     /translation="MATEPVNAPVPLLRVLEEEYEGLHGALPPTHAALNPHNRLAALY
                     TRIHQLPQKRTALCLSGGGIRSATFALGVVQALAHLSLLTRFDYLSTVSGGGYIGSWL
                     TAWIHRHPKGASGVMQDLRTVSFNDQDREPRPIKWLRQYSNYLSPRLGLLSADTWTLF
                     GSYLRNLTLNWLILVPLIAAVLVAPRMLTSLVRGTTGDLGLGPSGPGWSFNLGLLCGL
                     VALTYLHVYRPSLGSLRSHGIWRQLERQQWFLIAGLTPLIVAAFLLTNAWAWYRNAGG
                     LLDHLSFGSLSSRGTFVFGGALIHAAGWLVSALLLHRWRAISGRLLTELLVILWSGAV
                     GGLLLWSVLAETPLNIPVADYAEWYATFAVPAFLSMFLLTATLFVGVASRYTDDHDRE
                     WWGRAGAWTLIAMLTWSAGGALVIFGPGLLSYTPALMTSVGGVSGVISLVVGFSSRTA
                     ATGKTKPDLVDLILDRAVRLAAPLFIACLLVLVTLGTSWLLHLIAGLYEPGILDTQTI
                     GLGMPSDTWGHALILHNSPLLLLFELTLSLVVLAGVMSIVININKFSLHSVYRNRLIR
                     AYLGATRLPEYGVERRPNLFTGFDLSDNLQLHELVDTTGRIKKPFHVLNMAINLVRGS
                     NLAWQQRKAQSFTASPFSCGSWNLGYRRAKEYGANGRLHRAMTLGTAMAISGAAASPN
                     MGYHSAPAVTFLLAFFNVRLGWWLGNPGPAGRHTFDQSCPRIAVRPLFAETFGLTDAN
                     NPYVYLSDGGHFENLGLYEMVLRRCHCIVVSDASCDTARSFVDLGNAIRKIRIDLGID
                     IDIPTELLQVQPDNRLSRAHYALGIIRYDQVDREASPGLLIYLKPSLTGNEPADVRQY
                     ASCHDEFPHEPTADQFFDESQFESYRALGFHIAEKVFSQPLSKGTGQAEALFEAFRSG
                     QTG"
     gene            complement(5941..>6451)
                     /locus_tag="VFR82_01750"
     CDS             complement(5941..>6451)
                     /locus_tag="VFR82_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500352.1"
                     /translation="LGTESLTTLFLKKIPKGSSKSGLIIVIDTSFPFDAGGPGLDKSD
                     KGFEVFRDDPSRIVGIMEERANTYQTLLWHSLRTEGVALPDFAHLRIVVLKHIDAEWT
                     GYDDLPEACRNEFSTDVTPVAIKEAVSQIPTLFKIKTPCHGALLLKAARKVVEQSRHR
                     IVNFIEAHQ"
BASE COUNT         1486 a         1765 c         1901 g         1299 t
ORIGIN      
        1 ggaaaggcgt tattttgtgg ctgctcggag tgccagtgag cctactggtc gtgctgtggt
       61 ttcttgggat ccttcgataa ccacgcaccg agtgagatac tcacacataa aggagtgcac
      121 tatgttatcg ctgagacttg ggctgccgat gatcgctgcg attgcgttga gcgccgtacc
      181 ggcgttcgcc gctgatcttg aaacacaggt aatcgacaag aattgctaca tcgaagtctt
      241 cgatgatgac gatttcgata tggacgacgc ccattacaag atacaaggtc cgaaagaatt
      301 tgcgtcactg aaagacctgg gcggaaagaa ttggaacaat gacattgaaa gcgtgatcgt
      361 aggttccaac gcgaaggtca aagcttactc ggataaggat ttcaaaggga cagaactcgc
      421 atttgccccg ggccagaggg ttccaaatct cggtaaaatg aatatgaagg atgacatcga
      481 atcgatgaag attgcgtgcg gtagttgaac cctcacgccg ggagacggcc ggatggccgc
      541 ctctcggcgc tcctctctca ccttattatt gcccgcaaca tggcgttgct cccttgcggt
      601 cgcccttgaa gcggttgacc gtccgaccta caatgcttcg gcactgctct catcccttcc
      661 tgctcatgag gattgatgcc acggaaaaag ccttccccgg cagcggcgag atcggctgac
      721 ggttcagcga agaacgagga ttttgagcgg ctgggcgtct tctatcttgg tcgaccgtat
      781 gacccggtca tcaagcagcg gaaacccggg tggattctgt acgactcgaa ggacctggtg
      841 acccatgcgg tttgcgtggg catgacgggg agcggcaaaa cggggctatg tttgtcactc
      901 atcgaagagg ccgcgataga caatgtaccg gcgattctca tcgatccgaa gggcgacctc
      961 tccaatctat tactcacctt tcccggcctc accgctgaag aattccggcc gtggattaac
     1021 gaagacgagg cacgtaagaa agggcttccg ccggatgagt atgcggcgca gcaggcggat
     1081 cattggaaaa aaggtctcgc gtcctggcag gaggatggag cccgcatcaa gcggtttcag
     1141 gattccgccg acgcggtgat ttacacgccc gggagtcatg caggcttacc cgtctcgatt
     1201 ctcaaatcat ttgcggctcc tcccgacgat gtgcgcaacg acgacgaatt gttgcgggag
     1261 cggattagta cgaccgtgac cggtctgctg ggattgatcg gcgttgaagc cgatccgatt
     1321 caaagccgag agcatatcct gatttcgacg attctgaatg aaaaatggag gcgtaaagaa
     1381 gatcttgacc ttgcggccct cattcacgcc atccaatcgc cccccgccgg gaaaatcggc
     1441 gtcatggatg tcgatgcgtt cttcccttcc aaggaccggt ttgcgctctc gatgaagctg
     1501 aacaacctat tggctgcgcc cggcttccag gcctggctga gcggagcgtc gctggatgtc
     1561 caagaattgc tctacacgcc gacaggcaag cctcgtctgg caattttctc gatcgcacat
     1621 ctaagcgacg ctgaacggat gttttttgtc accttgttgc tgagccaggt ggtcggctgg
     1681 atgcgtggcc agcccggaac caccagcttg cgcgcgctgt tatatatgga cgagatcttc
     1741 ggctactttc cgccggttgc aaatccgccg tcgaagttgc cgttgatgac gttactcaag
     1801 caagcccggg cattcgggct gggagtggta ttggcgacac agaatccggt ggatcttgat
     1861 tataagggct tggccaacac cggcacctgg ttcatcggcc ggctccagac ggagcgggac
     1921 aaagcgcgtg tgctcgaagg gttagaaggc gcctctgtaa gcgcaggcaa gacattcgac
     1981 aagagtccca tggaacaaac gctggcagga cttggcgctc gcatcttctt gatgcataac
     2041 gtccacgaag acgagccggt cgtgttcgaa acgcgctggt ctctctccta cctacgaggc
     2101 ccgctcacgc gctcgcagat caagacctta atggatccgc gcagaaccga gtcacacaca
     2161 tcgaaggcgg cctctcaagc tgcaggcagc gctcggcaga gtacgctcga gtcgcggcca
     2221 ctagtggatc ccgatgtcgc gcaatatgtt gtgccgctgc gtggaagaag gcctgacgac
     2281 agtcgcctgg tctatgtgcc catggtgctg ggcgccggcc aggtgcgctt tgccgacagc
     2341 aagaacggca tcgatacgac acgggacttc catattgtag ttcctgtgac cgacggtgca
     2401 gtcgtcctcg attgggacca gggtgccgcg accacaatca caccggcaga tctcgagcaa
     2461 actcccgcag atgaagcgca gtttcttccg gtcccgtcca gcgccaataa agccaagagt
     2521 tatgacgtct ggaggaaaga gttcgcaggc tggctcttcc ggacgcaaaa attggagttg
     2581 ttgaagagtc aggcttcgaa cgaagtgtcg agaccgggag aatctgaacg tgactttcgt
     2641 gttcgtttac aacagggcgg tcgtgagcgg cgtgacgagc aaattgaatt gctccgccga
     2701 aaatacgcgc cgaagattgc ggcccttcaa gagcgggtcc gccgagctga ccaacagagg
     2761 gaaaagcaag aggtcgagtc gagatccagt caagtccaag ccgcaatttc ggtgggcgcg
     2821 tcaatcctcg gggcattctt gggcagaaag tccatcagtg cgacgaacat tggccgggcg
     2881 acgactgcaa ttcgcggcgc cggtcgtgtg ttgaaggaat cccgcgacgt gaatatggcc
     2941 gaagagaacg tggccgcgtt gcggcagcaa ctggcagatc tcgaagcgca gttcggatca
     3001 gaacgtgacg taattactgc ggcgaccgat ccacttcaag agaaactgga atccatttct
     3061 ctcaagccta ccaagacgaa cattaccgta aagctcgtcg cgttgacctg gactccgcac
     3121 tggcgatccg ccgccggcac gctgacgcct gcctgggaat agctcaagag gagtggtcag
     3181 ccggtttggc cggaacggaa tgcctcgaac agcgcttcgg cttgtccggt tccctttgac
     3241 agtggctgac tgaatacttt ctcggcgatg tgaaaaccga gtgccctgta ggactcgaac
     3301 tgcgattcgt caaaaaattg atcagcggtt ggttcgtggg gaaactcgtc atgacagctc
     3361 gcatattgcc tcacgtcggc cggctcattt cccgtcagcg acggcttcaa atagatcaac
     3421 aggccgggag aagcctcccg atcaacttga tcatagcgga tgatgcccag tgcgtagtgg
     3481 gctcggctca accggttatc cggctggact tgaaggagtt cggtgggaat atcgatgtct
     3541 attccgagat cgatgcgaat tttccgaata gcgttgccga gatccacaaa tgaccgggcg
     3601 gtgtcacaac tcgcgtcact gaccacgatg cagtggcacc ggcgaaggac catttcgtag
     3661 agtcccaggt tctcgaaatg gccgccgtcg gacaagtaga catagggatt gttcgcatcc
     3721 gtcaagccga acgtttcggc gaacaaggga cgcaccgcaa tgcgcgggca ggactggtca
     3781 aacgtgtggc ggccggccgg tcccggattg ccgagccacc agcccagcct gacgttaaag
     3841 aaagcgagca aaaacgttac agccggagct gagtggtaac ccatgttggg acttgctgcg
     3901 gctccggaga tggccatggc ggttcccaga gtcatggctc tgtggagccg cccgttcgct
     3961 ccatactcct tcgcacgtcg gtatccgaga ttccaactcc cgcaagagaa cggagaagcc
     4021 gtgaacgatt gcgccttccg ttgttgccag gccaagttgc ttccccggac gaggttaatg
     4081 gccatattga ggacatgaaa cggctttttg atgcgtccag tggtgtctac gagttcatgc
     4141 agctgcagat tgtcgctcag gtcgaatccg gtgaagaggt tgggacggcg ttcgacacca
     4201 tactccggaa ggcgcgtggc accgagatat gcccggatca gccggttgcg gtagaccgag
     4261 tgaagcgaaa acttattgat gttgatgacg atggacatga cccccgccaa aacgacaagg
     4321 gacaacgtga gttcaaacag caggagcaga gggctgttgt gaagaatgag cgcatgcccc
     4381 cacgtatcgg acggcattcc taaccctatg gtctgggtgt ccaggatgcc aggctcatac
     4441 aggcctgcaa tcaggtgaag aagccagctc gtgcccagcg tgaccaagac aagcagacaa
     4501 gcgatgaaca gaggagcggc cagccgaaca gcccgatcca gtatcaaatc caccaggtcg
     4561 ggcttcgtct tccccgtcgc cgcggtcctg gaactgaatc caaccaccag agagatcacg
     4621 ccagagactc ctccaacgct ggtcatgagt gccggtgtgt aggacagaag tccaggcccg
     4681 aatatgacca gcgccccccc ggcgctccac gtcaacatgg caatgagcgt ccaggctccc
     4741 gcccgtcccc accattcccg gtcgtgatcg tctgtataac ggctggcgac gcccacgaac
     4801 aacgttgcgg tgagaaggaa catcgacaga aaggccggca ccgcaaacgt ggcataccat
     4861 tcggcataat cggccaccgg aatgttgaga ggggtttctg cgagcaccga ccacagcaag
     4921 agacctccca cggcgccaga ccacaggatg accagaagct cggtcagaag acgcccactg
     4981 atcgcacgcc accggtgcaa cagcaaagcc gacaccagcc atccggcggc atgtatgagg
     5041 gcgccgccga atacgaatgt accgcgactt gatagactgc cgaacgacag atgatcgagc
     5101 aggccgccgg cgtttcgata ccaggcccag gcgttggtca gaagaaacgc ggcgactatg
     5161 agcggcgtca gaccggcaat aagaaaccat tgttgccgct ccaactgcct ccagataccg
     5221 tgagagcgaa gggacccgag acttgggcga tacacatgga gataggtcag ggcaaccaat
     5281 ccacacagca ggccgagatt aaagctccaa cccggtcctg atggcccaag accgaggtca
     5341 ccggtagtcc cgcggaccaa cgatgtcagc atacgaggag cgaccaacac ggcggcgatg
     5401 agaggcacga ggatcaacca attgagtgtc agattacgca gataactgcc gaagagagtc
     5461 caggtatcgg cggacaacaa tccgaggcgt gggcttagat agttgctgta ttgacgcaac
     5521 cacttgatag gcctcggttc gcggtcctgg tcattgaatg atacagtccg caagtcctgc
     5581 atcacgccgc ttgcgccctt tggatgtcga tgaatccatg cggtcagcca acttccaatg
     5641 taacctcctc ctgaaacggt cgagaggtag tcgaaccggg tgaggagtga gaggtgtgca
     5701 agcgcttgga cgactcccaa tgcaaacgtg gcgcttcgaa tccctcctcc agaaagacag
     5761 agggcggtcc gtttctgcgg aagttggtgt atgcgcgtgt aaagggcggc gagccgattg
     5821 tgaggattga gtgcggcatg tgttggaggc aaagctccat ggagcccttc atattcctcc
     5881 tcgagcacgc ggagcagggg aacgggagca ttgactggct cagtggccat gatggttcgt
     5941 ctattggtgt gcttcgatga aattgacgat tcggtgtcgc gactgttcca ccactttgcg
     6001 tgccgctttc aacagcaacg ccccgtgaca aggcgtcttg atcttgaaca aggtcgggat
     6061 ctggctgacg gcttccttga tcgcgaccgg cgtcacgtcg gtggaaaatt cattccgaca
     6121 tgcttcgggc aggtcatcgt atcccgtcca ctctgcatcg atgtgtttta agacgacaat
     6181 tctcagatgg gcgaaatctg gaagcgcgac gccttcggtc ctgagactgt gccagagcag
     6241 cgtctggtag gtatttgccc gttcttccat aatgccgaca atacgcgagg gatcgtcccg
     6301 aaagacctcg aatcctttgt cgctcttgtc gaggccgggc cctcctgcgt cgaagggaaa
     6361 tgatgtatcg atcacgatga tcagaccgct ttttgaagac cccttaggga tttttttcaa
     6421 gaagagggtc gtgagggatt cggtccccag a
//
LOCUS       DASTQY010000053         4132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1780179, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000053 DASTQY010000000
VERSION     DASTQY010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780179"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VFR82_01755"
     CDS             complement(<1..660)
                     /locus_tag="VFR82_01755"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU4500353.1"
                     /translation="MAWFKKQKGSEPEAPKRSKVAEGMWLKCNHCREIVYRKEVERNS
                     KVCPKCDYHFPISVIERITLLVDFGTFREWDAELGPLDPLNFHDTKSYKDRVKAQQEK
                     TGKKDAIMIGEGQMNGRRVALCLFDFGFMGGSMGSVVGEKICRAIDRAIEARLPVLLV
                     TASGGARMQEGILSLMQMAKTSAAVAKLGEAKLPLICLLADPTFGGVTASVAMLGDVI
                     IA"
     gene            complement(817..1524)
                     /locus_tag="VFR82_01760"
     CDS             complement(817..1524)
                     /locus_tag="VFR82_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU4500354.1"
                     /translation="MTDPSYLTVVGLGFVLGLRHALDSDHLAAVSTVLAERPSWRASG
                     LVGLSWGLGHTAMLLLVGAIVLFLRVPIPQPVADVAECVVGLMLVVLGGLLGMKLVRE
                     RWHLHQHDHDGTRHVHLHSHAQSGDHGHAHWWRESVRPLCIGMAHGLAGSAALLLFVV
                     ASAGSVLEGLIYIAVFGCGSIAGMMLIGLLLSIPVVWSLRLGRPVFLTLQGLASAGSI
                     ALGLSIVYRTLTGESLS"
     gene            complement(1730..2797)
                     /gene="moaA"
                     /locus_tag="VFR82_01765"
     CDS             complement(1730..2797)
                     /gene="moaA"
                     /locus_tag="VFR82_01765"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEU4500355.1"
                     /translation="MDSRELNKDPLQTVVDTFGRPLKSLRLSVTDRCNLRCAYCMPEE
                     EYVWLPREDVLTFEEMALLTGYFTDLGVDKVRLTGGEPLLRRDLPRLIRLLLQNRNIK
                     EIALTTNGVLLSDHAEALYQAGLNRVTVSLDTLRPERFRQLTRRDEFARVLEGIESVG
                     RAGFIGLKLDTVAIQGFNDDELIGLIEFGKHYQAEVRFIEYMDVGGANEWVPEKVLSR
                     EMILAALGKYYGSIQPLPERGSAPAQRFRLPDGAAFGIIPSTTTPFCSTCDRSRVTAD
                     GMWYRCLYATAGTDLRKPLREGVPGDEMRALIRGGWESRRDRGAEERKALERAGLREG
                     GLIGIDRLREDPHLEMHARGG"
     gene            complement(2894..3592)
                     /locus_tag="VFR82_01770"
     CDS             complement(2894..3592)
                     /locus_tag="VFR82_01770"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEU4500356.1"
                     /translation="MITVKLFGMTKLLAGNESTLTVALAKGHSVKNLIELVNKRYPKI
                     GELIYKKKVLVSVNQEIAHEDTKVQEGDEVALLPPFAGGTQMASAIDDSQLVRVQRED
                     FSIDTELHRVRSRSKRIGGIAMFLGTARDRSQGRDVDGITFEHYEGMAQKKLREIRAR
                     ALKDFDILELLIIHRFGAITIGENIVLIIAGAEHRAEAFRACKWAIDELKQITPIWKL
                     EHTPTGEVWVEEHP"
     gene            complement(3641..4129)
                     /gene="moaC"
                     /locus_tag="VFR82_01775"
     CDS             complement(3641..4129)
                     /gene="moaC"
                     /locus_tag="VFR82_01775"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015428892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEU4500357.1"
                     /translation="MAEFTHFNESGRARMVDVSAKAQTERVATAQAKVYLQPETLEKI
                     QRGKIAKGDVLAVAQVAGVMGAKKTPELIPMCHPIFLSAIDISFKEEPQPDRDGRCSI
                     TVTATAKTSGQTGVEMEAMTAVSIAALTIYDMCKAIDRAMSFSDVWLLSKAGGKSGTY
                     TR"
BASE COUNT          897 a         1271 c         1083 g          881 t
ORIGIN      
        1 ggcaatgatg acatcgccaa gcatggcgac actcgccgtc accccgccga acgttggatc
       61 cgccaggaga cagatcaatg gaagcttggc ctcccccaat ttcgctaccg ccgctgacgt
      121 cttggccatc tgcatgaggg acagaatgcc ttcctgcatg cgggcaccgc ctgaagcggt
      181 caccagcaga actgggaggc gagcttcaat cgcgcgatcg atcgcacggc aaatcttttc
      241 gcccaccacc gaacccatgc tgccgcccat aaatccgaag tcaaagagac agagcgccac
      301 tcgtcggccg ttcatctggc cttcaccgat catgatggca tctttttttc ccgtcttctc
      361 ctgctgggcc ttgacccgat ctttgtagga ttttgtgtcg tgaaagttca atggatccaa
      421 cgggccgagt tcggcatccc attccctgaa agtcccgaaa tctaccagaa gggtgatacg
      481 ctcgatgacc gaaattggaa aatgatagtc gcatttcggg cagactttgc tattgcgttc
      541 gacttcttta cgatagacga tttcacggca atggttgcac ttgagccaca tcccttcggc
      601 cacttttgac cgtttagggg cttccggctc cgaacctttt tgtttcttga accatgccat
      661 ggcaggctcc ttatacgatt caatcaatcc ggggccgtcg gaggagtcaa ggaacttgcc
      721 tgtcgcaaga gaacccgtcg gtcgggtgtg gagaatacca tagcggggcg cgtggcgcac
      781 gtagaattcc cctcgcgcga caaatgaacg atctcgtcag gacaaggact ctccggtaag
      841 tgtacgatag acaatcgaga gacctaacgc gatacttccg gcactagcca gtccctggag
      901 tgtaagaaat accggacgcc ccagccgcaa ggaccagaca acgggaatgc tcaggagcaa
      961 tccgattagc atcatgcccg caatagaacc gcagccgaag acggcaatgt agatcaatcc
     1021 ttccaggaca ctgcctgccg atgcgacgac aaacagcaac agcgcggcag agccggcgag
     1081 gccatgcgcc atcccgatgc agagcggccg cacggactcg cgccaccaat gcgcgtgacc
     1141 gtgatctccc gactgcgcat gactgtgaag atgcacatgc ctcgtgccgt catggtcgtg
     1201 ttgatgcagg tgccagcgct ctctcaccaa cttcatgccg agcagaccgc caagcacgac
     1261 gagcatgagt ccaaccacac attccgccac gtctgcgacc ggttggggaa tcgggacgcg
     1321 caagaacagg acgatggctc cgacaagcag caacatcgcc gtgtgaccga gtccccaact
     1381 cagccccacg agcccggacg cccgccatga cggccgctct gcgagcaccg ttgagacagc
     1441 cgccagatgg tctgaatcca gcgcatgacg aaggccgagc acaaacccca gtccgaccac
     1501 cgtcaagtag gaagggtcag tcatgagaca ccgagattaa tccaacggct ggtgcgtgtg
     1561 agaccgtgag agggaacgga aagtaactgc ggcatgacgt gtcatctcgc aggatggtca
     1621 tcaggccgtc cggcaaggcc acgactgaag agccggctgg tacacggact gaaatctcga
     1681 agcactgagc gatgcaggtg cgtcgccggg aggcatctcc aacatgcggc taaccaccgc
     1741 gcgcgtgcat ttccaaatgc ggatcttccc gcaaacggtc gataccgatg agcccacctt
     1801 cccgcaaccc cgcgcgttcc agtgccttgc gttcttccgc ccctcggtca cgccgtgatt
     1861 cccatccgcc tcgaatcaaa gcccgcatct catccccagg cacaccctca cgaagcggct
     1921 ttctcaagtc agtgccagct gtcgcgtaga gacagcgata ccacatcccg tcggccgtca
     1981 cccggcttcg gtcgcaggtc gaacagaatg gcgtggtggt ggaagggatg atgccgaatg
     2041 ccgcgccatc cggaagccgg aagcgttgag cgggagccga tcctcgctcc ggcaagggtt
     2101 gaatgctccc gtaatatttg cccagtgccg caaggatcat ctctcgagac aggacctttt
     2161 ccggaaccca ttcgttagcg ccgccgacat ccatgtattc gatgaaccgc acttcggctt
     2221 gataatgctt gccgaattca atcagtccga tcaattcgtc gtcgttgaaa ccttgaatcg
     2281 caacggtatc gagcttgagt ccgataaagc ccgcccggcc aactgactca atgccttcca
     2341 ggacccgggc gaattcgtcc cttcgtgtga gctgcctgaa gcgctccggt ctcaatgtat
     2401 cgagagagac ggtcacgcgg tttaaaccgg cctgatacag cgcctcggca tggtcagaca
     2461 ggaggacgcc attggtcgtc agggcaatct ctttgatgtt gcgattctga aggagcaaac
     2521 gaatcagtct tgggagatcc cggcgcagca acggctctcc gccggtcaat ctgactttgt
     2581 cgacgccgag atccgtaaag tagccggtca gcagggccat ttcctcgaag gtgagcacat
     2641 cctcccgagg cagccacaca tattcttcct caggcataca ataggcgcat cgcaagttgc
     2701 accggtcggt cactgagagc cgcaagctct tcagcggacg accgaaggtg tcaacgactg
     2761 tctgaagagg gtctttatta agttctcttg agtccacaga gctgtctttc cctgaggatg
     2821 gtcaacatgt tgtccttctc acccgcccac cctggcgcgc caagacgtgc cgctcccaag
     2881 cacggcatcc tctttaaggg tgttcttcga cccagacctc tcctgtcggc gtatgctcca
     2941 gtttccagat cggagtgatt tgcttcaact cgtcgatggc ccacttacac gcccgaaacg
     3001 cttctgcacg gtgttccgcg cccgcgatga tcaagacgat gttttcaccg atcgtaattg
     3061 ccccgaagcg atggatgatc agcaattcaa gaatgtcgaa atccttcaac gccctcgccc
     3121 gaatctcccg cagtttcttt tgagccatcc cttcgtagtg ctcgaaggta atgccgtcga
     3181 cgtcacgtcc ctgcgaacgg tcacgtgcag tcccgaggaa catggcaatg cctccgatac
     3241 gcttggatcg gcttctgaca cggtgcaatt ctgtatcgat cgaaaaatcc tcccgctgca
     3301 cacgtaccaa ttgactgtca tcgatggctg aggccatttg agttccaccg gcgaatggag
     3361 gcagcaaagc gacctcatcc ccttcttgga ctttggtatc ctcgtgggcg atttcctgat
     3421 tgaccgatac caggactttt ttcttataga tcagttcacc gatcttggga taccgcttgt
     3481 tcaccagttc aatgagattt ttcacggagt gccccttggc gagggcaacc gtcagggtgg
     3541 attcgttccc cgcaagcagc ttcgtcattc cgaataactt gacggtgatc atcgacactc
     3601 actcctcccg agtcgccgcc tctctctcgt cgttcgcgtc ttatctggta tacgtcccgg
     3661 acttcccgcc ggcctttgac aaaagccaga catcgctgaa actcattgcc cggtcgatcg
     3721 ccttgcacat atcgtagatg gtcagagcag cgatggacac ggcggtcatc gcttccatct
     3781 cgacccccgt ctgcccgctc gtcttggctg tggccgttac cgtgatagaa caacgtccat
     3841 cacgatcggg ctgcggttct tccttgaacg aaatatctat cgccgaaagg aagatcggat
     3901 ggcacatcgg aatcaactcg ggggtctttt tggcccccat gacgcctgct acttgggcga
     3961 ctgcgagtac atccccttta gcaatctttc cccgttgtat tttttcaaga gtttcgggct
     4021 ggagatagac cttcgcctgg gcggtcgcca ctcgttcggt ctgggccttg gcgctcacat
     4081 caaccatccg cgcccgaccc gactcgttaa agtgtgtgaa ctcggccatt tt
//
LOCUS       DASTQY010000054         3582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1172733, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000054 DASTQY010000000
VERSION     DASTQY010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172733"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VFR82_01780"
     CDS             <1..466
                     /locus_tag="VFR82_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEU4500358.1"
                     /translation="TVVDDPKPETLNEIHGLKRQLLFVRRAVWPLRDVMNNLSRSDCP
                     FLQEATKLFFRDVYDHVVQIVDTIETLREMVSASLDIYLSSVSYRLNAVMRVLTVITT
                     IFMPLSFIASIYGMNFEHMPELKSTWGYPIVLAAMTAVGLGMLLLFRNKRWL"
     gene            complement(618..974)
                     /locus_tag="VFR82_01785"
     CDS             complement(618..974)
                     /locus_tag="VFR82_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500359.1"
                     /translation="MWLFRPRFFLVLLFLAAGVLLVAFNYSYSDGNRAGYVQKFSKKG
                     WFCKTHEGELAMTTVPGTAPVLWEFTVWDDAVADLLSEAMGKRVVLHYKEYRYLPTTC
                     FGDTPYFVDRVEVQND"
     gene            complement(1017..1640)
                     /gene="leuD"
                     /locus_tag="VFR82_01790"
     CDS             complement(1017..1640)
                     /gene="leuD"
                     /locus_tag="VFR82_01790"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU4500360.1"
                     /translation="MQAFTELTGLVAPLDRVNVDTDQIIPKQFLKTIKRTGLREGLFF
                     DWRKQKDGSPDPLFFLNQPRYQGATILLTRDNFGCGSSREHAPWALLDQGFRCVIASS
                     FADIFYSNCFQNGILPVVLQADAVESMMRDVMAMPQYRATIDLGQQTVTTPDGTVYGF
                     SIDPFRKDCLYRGLDSIGLTLEHEPAIAAYEARRRAEAPWLFSDFPS"
     gene            complement(1674..3077)
                     /gene="leuC"
                     /locus_tag="VFR82_01795"
     CDS             complement(1674..3077)
                     /gene="leuC"
                     /locus_tag="VFR82_01795"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEU4500361.1"
                     /translation="MAGQTLFDKIWESHVVRAERDGTTLLYIDRQLVHEVTSPQAFEG
                     LKLAGRRPRRPGATLAVPDHNVPTTDRRLGIEDRVSALQIQTLEDNCRELGITLFNMS
                     DIRQGIVHVIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLI
                     QKRPKTMEVRVDGVLSDRCFAKDVILAIIGEIGTAGGTGYVIEYTGSVIRGLSMEGRM
                     TLCNMSIEAGARAGMVAPDEKTFAYIKGRPLAPTGAAWDQAVRAWQHLKTDEDATYDA
                     TVVVKAEDIAPQVSWGTSPGMVTGVDGKVPDPKTIVDEKARQAAERALAYMGLKANMP
                     ITDIRIDRVFIGSCTNSRMEDLRLAASLAKGKHIAKTVRAMVVPGSGLIKQQAEAEGL
                     DRVFTAAGFEWREPGCSMCLAMNADVLQPGERCASTSNRNFEGRQGAGGRTHLVSPAM
                     AVAAAVEGHFVDIRRWS"
     gene            3208..>3582
                     /locus_tag="VFR82_01800"
     CDS             3208..>3582
                     /locus_tag="VFR82_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HEU4500362.1"
                     /translation="MNQTIVQWTQRLLDSYRHWMGTELLERTGDPELQAHALFESPVV
                     VVSHGTQTDPVLNYGNRMALKLWEMTWEQLIETPSRLTAEPVNRAEREWMLEQARVRG
                     YLDTYRGVRVSRSGRRFLVEGAV"
BASE COUNT          742 a         1055 c         1037 g          748 t
ORIGIN      
        1 aacggttgtc gatgatccca agcccgaaac gctcaacgag atccatgggc tcaagcgcca
       61 gctgctgttt gtacgacggg ccgtctggcc cctacgggat gtgatgaaca acctgtcgcg
      121 ttcagactgt ccgttcctcc aggaagccac caagctgttc ttccgcgacg tctacgacca
      181 tgtcgtgcag atcgtcgata ccatcgaaac gctgcgcgag atggtctcgg ccagtttgga
      241 tatttatctc tcaagcgtca gctatcgcct gaatgccgtc atgcgggtgt tgaccgtcat
      301 taccaccatc ttcatgccgc tcagctttat cgccagcatt tatggcatga acttcgaaca
      361 catgccggag ctgaaatcga cgtggggtta tccgatcgtg ctggccgcga tgacggcggt
      421 tgggctgggg atgctgctct tgttcaggaa caaacggtgg ctgtaggggt gcccgctctc
      481 tcccgctgca agccaagggt acagccaaaa cattttcccc tcagaccctt agagcgttgg
      541 actcggtatg cgaacgatgg cctgctcgca ggccaggctc actgaagtgt cgtgggtatg
      601 caggacgaag tgtccgacta gtcgttttga acctcaacgc gatccacgaa gtacggcgtg
      661 tcgccgaagc aggtcgtggg cagataacgg tattctttgt aatggagtac gacgcgtttg
      721 cccatcgcct cggatagcag atccgccaca gcatcgtccc agactgtaaa ttcccacagg
      781 acaggcgcgg tgccggggac cgtcgtcatg gcaagttctc cctcatgggt cttgcaaaac
      841 catccttttt tggaaaattt ctgaacgtag ccggcacgat tgccgtcaga gtaactgtag
      901 ttaaaggcga cgaggagcac gccggcggcc aggaacaaaa gtactaagaa gaagcggggt
      961 ctaaacagcc acatgggaaa cacaatcgcc gcccagttcg tgcggtcctt ccagactcag
     1021 gatgggaaat cggagaacaa ccacggggcc tcagccctgc gccgggcttc ataggctgcg
     1081 atcgccggct cgtgctctaa cgtcaagccg atcgaatcca gtccgcgata caggcaatcc
     1141 ttccggaacg gatcaatcga gaatccatac actgtgccgt cgggagtcgt cacggtctgt
     1201 tgaccgagat cgatcgtggc ccgatactgt ggcatcgcca ttacgtccct catcatcgac
     1261 tccacggcgt cggcttgcaa aacgaccggc aggataccgt tctgaaaaca gttgctataa
     1321 aaaatgtccg caaaactgga cgcgatgacg caacggaatc cctgatctag taaagcccaa
     1381 ggcgcgtgtt cacgcgacga gccgcagccg aagttgtcgc gggtcaaaag gatggtggca
     1441 ccctggtacc ggggctgatt caagaagaac aaaggatccg gagaaccgtc cttctgcttg
     1501 cgccagtcaa aaaacaggcc ttcgcggagc ccggtgcgct tgatcgtctt gagaaactgc
     1561 ttcgggataa tttgatcggt gtcgacattg acccgatcca gtggagccac caggccggtc
     1621 aattcagtga acgcttgcat caacgtcctt gcgtgagaat gtacggccgg tcgctatgac
     1681 cagcgcctga tgtcaacaaa atggccttcg actgctgcag ccacggccat ggccggcgaa
     1741 accaaatgcg tccggccgcc cgcgccttga cgaccttcga agttgcgatt gctcgtggag
     1801 gcgcagcgct cccccggttg cagtacatcg gcattcatag cgaggcacat gctgcaaccc
     1861 ggttctcgcc actcgaaacc cgccgccgtg aagacgcgat cgagtccttc ggcttcggcc
     1921 tgctgtttga tcaggcctga tccaggaacc accatcgccc ggacggtctt cgcgatgtgt
     1981 ttccctttcg ccaggctggc ggcaagccgc agatcctcca tgcgagagtt cgtgcacgaa
     2041 ccgatgaaga cgcgatcgat ccggatgtcg gttatgggca tgttcgcctt taggcccatg
     2101 taagccaggg ctcgctcggc cgcctgccgg gctttctcat caacgatggt tttgggatcc
     2161 ggaacctttc cgtcgacgcc cgtcaccata ccggggcttg tcccccaact cacttgcggg
     2221 gcgatgtctt cagctttcac gaccacggtg gcatcatagg tcgcatcctc atcggtcttg
     2281 agatgctgcc acgcccgaac ggcttgatcc catgcagcgc cggtcggtgc gagtggacga
     2341 cccttgatgt aggcaaaggt tttttcatcc ggggcgacca tgcctgcgcg cgcaccggct
     2401 tcgatcgaca tgttacacag cgtcatgcgc ccttccatgc tgaggcctcg gatcacggaa
     2461 ccggtgtact caatgacata ccccgtcccg ccggcggttc cgatttcccc gataatggcg
     2521 aggatgacgt ccttcgcaaa gcatcgatcc gagagcacgc cgtcgacacg cacctccatg
     2581 gtcttcgggc gcttctgaat cagacattgc gtggcaagca catgctcgac ttcgctggta
     2641 ccgatgccga aagccaatgc gccgaaggcg ccgtgcgtcg acgtatgaga gtctccgcac
     2701 acgatggtgg taccggggag cgtaaaaccc tgctccggac cgatcacatg gacgatgccc
     2761 tggcggatgt cgctcatgtt gaacagcgta atgccgagct ctctgcagtt gtcttccagc
     2821 gtctgaatct gtagggcgct gacccggtct tcgatgccaa ggcgccggtc cgtggtcggc
     2881 acattgtgat cgggtacggc caaggtggcg cctggacggc gaggtcggcg accggcgagt
     2941 ttcaatcctt cgaaggcttg tggcgacgtg acttcgtgaa ccagttgtcg gtcgatgtag
     3001 agcagtgtcg tcccatcgcg ctcggctcga acgacgtggg attcccaaat tttgtcgaac
     3061 agcgtctgac ccgccatctc ctctctaccc catgaccttt cttgcgaggc gtcattatac
     3121 agagactgcc tataggatgt acaagagcgt ttgccttgcc ctccagggac gtgctaccac
     3181 gctgtcggca tgagttctca gccctggatg aaccagacca ttgtgcagtg gacgcagcgg
     3241 ctgctcgaca gttaccggca ttggatgggg acagaattac tcgaacgaac cggagatcct
     3301 gaactgcaag ctcacgcctt gttcgagtcg ccggtggtcg tggtgtccca cggcacgcaa
     3361 acggatcccg tgttgaatta cggcaacagg atggccctca aattgtggga aatgacctgg
     3421 gagcaattga tcgagactcc atcgcggctg accgccgagc ctgtcaaccg tgctgagcgg
     3481 gaatggatgc tcgagcaggc gcgcgtccgc ggatatctcg atacgtatcg gggcgtgaga
     3541 gtatcgcgaa gcggccggcg cttcctagtc gaaggcgcgg tc
//
LOCUS       DASTQY010000055         3585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_166155, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000055 DASTQY010000000
VERSION     DASTQY010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166155"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /gene="bioB"
                     /locus_tag="VFR82_01805"
     CDS             <1..650
                     /gene="bioB"
                     /locus_tag="VFR82_01805"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEU4500363.1"
                     /translation="DEIAGAVREIKQELPIQICCSLGLMNEGQARRLKAAGVDRVNHN
                     LNTSESFHRSICTTHTFQDRLATIRNARAAGLEICSGGIVGMGEQDDDLIDLAMALRD
                     VKPDSIPLNALHPVGGTPLEHCDHLTPQRCLKILCLFRFLHPRTEIRIAGGREHNLRS
                     LQPLALYPADSVFVNGYLTTPGAPAPEVWGMIRDLGFTIEVDYQPAPADSLAVQR"
     gene            828..1973
                     /locus_tag="VFR82_01810"
     CDS             828..1973
                     /locus_tag="VFR82_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU4500364.1"
                     /translation="MYPQVIPTKAQDSPLTTVNLPTDSRSLAEVIVAVVATIFALYWA
                     QRFFIPLFLGIILAYTLNPLVTWMERIKIPRLVGTTIVMMTLLFGVALGTLSLRGQVE
                     RILDQVPDAAGKLSVALKDMATQPNTMLKVQAAAREIEQATNQAANGTAASQKPPTRV
                     VIEEPKFKLLNWLWTGSLGMFGIIGETAMMLILVVFLLLSGDTFKRKLMRISGPSLSK
                     RKITAAILDDIDRSIQNYMLTLLMANVLLGVLTWLAFRLVGLDNAGAWAVAAGVLHFI
                     PYLGPALTAVATGMAGFMQFESLSMAMLVAASSLIIAMLVGMVLVTWMCGKISNMNTT
                     AVFVALLFWGWLWGIWGLLFAIPITGMVKVFAERVEDLRPVAELLSD"
     gene            1966..2838
                     /locus_tag="VFR82_01815"
     CDS             1966..2838
                     /locus_tag="VFR82_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500365.1"
                     /translation="MIDLPVTLRARLTARMRRCFPYLCSLGIIAAFFVTLRETEARPI
                     AVRFLEGLTHGFLLVRSSAGEIIGDGELTQVMKEGVAESRLVFRFKDGSLHDERVAFS
                     QQRVFTLIRYQLVQRGPSFPEQIDVTVDRATSKYEVRSRMRENDNEKVLAGDIDLPKD
                     AYNGMIVTTLLNLPKASSESVQVLAFTPKPMAIPLELSFVGEQMIRVGEQSRKALQYT
                     FKPDIGPIKKFLGKVLGKLPADFHYDCLILADDVPSFVRFQGPLQLMGPILSIELTSP
                     QAAGQPGDQKHVSK"
     gene            2853..3098
                     /locus_tag="VFR82_01820"
     CDS             2853..3098
                     /locus_tag="VFR82_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500366.1"
                     /translation="MNPIFSLLIKQLPRLLPVVKSMLERPPSKSTEEGRLAGIEQSIE
                     WLAERSDAVERRLKRITILVVAGLLLSVVAFVLMLVR"
     gene            complement(3104..>3585)
                     /locus_tag="VFR82_01825"
     CDS             complement(3104..>3585)
                     /locus_tag="VFR82_01825"
                     /inference="COORDINATES: protein motif:HMM:NF021511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEU4500367.1"
                     /translation="WLTLHTVAVHYTYPKVPVGDWLDRWFDFHRNHFDRIVHFSFGFL
                     MTLPLVEVFRRRCNAKGWLLPYLVVMTVLGFSAFWEIVEAWIGQFAHPDIEKAMVGHQ
                     GDVWDPQRDMASAFYGSLVYVGYLAWSRALHEEQATPECVSEPDGMLNPRMDSGQGV"
BASE COUNT          789 a         1019 c          983 g          794 t
ORIGIN      
        1 tcgatgagat cgccggtgcc gtccgagaga tcaagcagga gctgccgatc caaatctgct
       61 gctccctggg attgatgaac gaaggccaag ccagacggct gaaggctgcg ggagtagatc
      121 gtgtcaacca caatttaaac acgagtgagt cctttcaccg ctccatctgc accacgcaca
      181 ccttccagga tcgtctcgcc acgatacgaa acgcgcgggc agccggcctc gaaatatgct
      241 cgggcggaat tgtcggaatg ggagagcagg atgacgatct catcgatctc gccatggcgc
      301 tacgcgacgt gaaaccggat tccatcccgt tgaatgccct gcatcccgtg ggaggtactc
      361 cgctggagca ttgcgaccac ctcactccac aacgctgtct aaaaatattg tgtctcttcc
      421 gtttccttca cccccgcaca gaaatccgca ttgccggcgg tcgagaacat aacttgcgca
      481 gcctgcaacc cttggctctc tatccggcgg attctgtctt tgtaaacggg tatttgacca
      541 cccccggcgc gccagcgcca gaggtgtggg gcatgattcg tgatctcggg ttcaccatcg
      601 aagtggacta ccagccggct cccgctgact cgctcgccgt gcagaggtaa cttttcccca
      661 atcgcgtgtg cttcgcttca tctgtcctgt acctttccac cgtagacaag gacttctcct
      721 agtagggatt tcccctggtg ctgtggtgtc atgggtgtag agcatctctt accggactcc
      781 gatgcggtat ggccacagac gaacgtacaa caacgtgctc tcccggcgtg tacccacagg
      841 tcataccgac gaaggcgcaa gactccccgc ttacaacggt taatctgcca acggattcac
      901 gaagcctggc agaggtcatt gtcgctgtcg ttgcgaccat ttttgcgctc tattgggccc
      961 aacggttttt tattccgctc ttcctcggta tcatcctcgc ctacacattg aatcctctgg
     1021 tgacatggat ggaacggatt aaaatacccc gtctcgtcgg gacgactatc gtaatgatga
     1081 cattgctgtt tggtgtagcg ctggggactt tgtcgctccg cggacaagtc gagagaatct
     1141 tggatcaggt gccggatgcg gccgggaaat tgtcggttgc gctcaaggat atggccactc
     1201 agcccaatac catgctgaag gtccaggcgg cggctcgaga gattgagcaa gcaaccaacc
     1261 aggccgccaa tgggacggcc gcttcgcaaa aacctccgac ccgtgtggtg atcgaagagc
     1321 cgaaattcaa actccttaac tggctctgga cgggatctct cgggatgttc ggcatcatcg
     1381 gcgaaacagc gatgatgctg atcctcgtgg tatttctcct gttatccggt gacacattca
     1441 agcggaaatt aatgcgcatc agcggccctt cgttatcgaa gagaaagatc accgccgcga
     1501 tcctcgacga catcgatcga tccatccaga attacatgct cacgctcctg atggccaatg
     1561 tcctgcttgg agttctgacc tggctcgcgt tccgcttggt cgggctggac aacgccggcg
     1621 catgggcagt cgcggccggt gtgttgcatt tcattcctta tcttgggccg gcgctgaccg
     1681 ccgtggccac tgggatggcc ggcttcatgc aattcgagtc cttgtccatg gccatgctcg
     1741 tcgcggccag ctctctcatc atcgccatgc tggtcggcat ggttctcgtc acatggatgt
     1801 gcggcaagat ttcaaacatg aacacgactg ccgtgtttgt cgcgttgctg ttttgggggt
     1861 ggctctgggg aatatggggg ctcttgtttg ccattcctat cacaggaatg gtgaaggtgt
     1921 ttgcggagcg cgtcgaagat ctgcggccgg tcgcggagtt gctgagtgat tgatctaccg
     1981 gtcacattac gcgcgcggct aaccgccagg atgcgacgtt gtttccccta tctttgcagc
     2041 cttggcatca tagcggcgtt tttcgtcaca ctccgtgaaa cggaggcccg tccgatcgcg
     2101 gtacgatttc ttgaagggtt gacgcacgga ttccttctcg ttcggtcctc tgccggtgag
     2161 atcatcgggg atggcgagct gacacaagtg atgaaagaag gcgtggccga aagccgtctg
     2221 gtgttccgct tcaaagacgg gtcgctccat gatgaaagag tcgcgttttc acaacagcgg
     2281 gtgtttaccc taattcgcta tcaactggta cagcgaggac cgtcctttcc cgagcagatc
     2341 gatgtgacag tcgatcgagc gacatccaaa tacgaagtcc gatcccggat gcgagaaaac
     2401 gacaacgaaa aagttctcgc aggagacatc gatctgccca aggatgccta caacggcatg
     2461 attgtcacga ccctcttgaa cctaccaaag gcctcaagcg aatcagtgca agtgcttgcc
     2521 tttacgccaa agcctatggc tatcccgctc gaattgagct tcgtcggtga acaaatgata
     2581 cgagtgggag agcagtcccg gaaggcattg caatacacct tcaaaccgga tatcggcccg
     2641 atcaaaaaat tcctagggaa ggtgctcggg aagttgccgg ccgacttcca ctacgattgc
     2701 ctcattctgg cagacgacgt tccgagcttc gtgcggttcc aaggacctct gcaactgatg
     2761 ggaccaattc tgagcattga actaacaagt cctcaagcgg cgggacagcc cggggaccag
     2821 aagcatgtgt ccaaataacg ggcaagcctc acatgaatcc cattttcagc cttttgatca
     2881 agcaactgcc gagactactt ccagtcgtga aatccatgct ggaacgacca ccgtcgaaaa
     2941 gcacggaaga agggcgactg gcgggtatcg aacaatccat agaatggctt gccgaacgtt
     3001 cagatgcagt cgaacggcga ctcaagcgga tcacgatact ggttgtcgca ggactgcttc
     3061 tctctgtggt cgcgttcgtg ctgatgctgg tccgttagat ccgctaaacc ccttgtccag
     3121 agtccatgcg gggattcagc atgccatcgg gctcgctcac acactcaggg gtggcctgct
     3181 cctcgtgaag cgccctgctc caggcaagat aaccgacata caccagggag ccatagaagg
     3241 cggatgccat atcgcgctgt ggatcccaga cgtccccctg atgtccaacc atggccttct
     3301 cgatgtcggg atgcgcgaac tgcccgatcc aagcttcgac gatttcccag aaggcgctga
     3361 aacccaatac ggtcatcacg accagatagg ggagcaacca gcctttggca ttgcaccggc
     3421 gtcgaaacac ttcgaccagt ggcagggtca tcagaaagcc gaagctgaaa tgcacgatcc
     3481 gatcgaaatg attgcggtga aagtcaaacc accggtcgag ccagtctccg acgggtacct
     3541 tgggataggt atagtgcact gcgaccgtgt gcagcgtcag ccaca
//
LOCUS       DASTQY010000056         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1775118, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000056 DASTQY010000000
VERSION     DASTQY010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775118"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1039
                     /locus_tag="VFR82_01830"
     CDS             77..1039
                     /locus_tag="VFR82_01830"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU4500368.1"
                     /translation="MAQQARRSRPSRIQRSERPWQMVKSLAKKSVDKWNDEPGPRFAA
                     ALAFYTAFTIVPFVVLVVMVSATFMGEEAIKGDLHKHVGDLIGEPSADGLFHLIDQWR
                     AAGSPVLNELVALGVFLLGTVHVMDQLRDALDCIWGLKTKRPRAMMARLKQRFASVSG
                     LLGIAFILLSSLTLSAWLNVGIQSTFGGIGGPMWMQKAIGTAMSFLIIAALFALIYKW
                     VPQAKVAWADVWIGALITGALYSVGSMLIVVKLGHSPLVTFYGGAGSLVLVLLWAYYA
                     SQIFLFGAAFIAVYASQYGSHVRPTEGAVAVHSAAKGESADEDD"
     gene            complement(1044..1415)
                     /locus_tag="VFR82_01835"
     CDS             complement(1044..1415)
                     /locus_tag="VFR82_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500369.1"
                     /translation="MRSPGVLWIKAAVVYLVTGVGLGIYMGASGDHVMVPVHAHFNLL
                     GWASLALVGLIYHQFPHAGASRLATVQFWLHNVGLLAAMALLISALRGNTGLDPFLGL
                     ASMVIGSAIVLFAINVFQRVR"
     gene            complement(1507..2070)
                     /locus_tag="VFR82_01840"
     CDS             complement(1507..2070)
                     /locus_tag="VFR82_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500370.1"
                     /translation="MATHQFAVDREERVDTRSALLAAPGRSPEIPESADAYGWLVGSW
                     NLDIRVYWATDVSAQRLKGEAHFGWALEGRAIQDVWIMPCVADRTANLDKRMNMYGTT
                     LRVWDPSIQAWRITWINPAGDHREEQIGRWSGKNVVQVGVRPDGTPTRWMFSEITRDS
                     FHWTGEALNPDGHTWKLEGDFRVTRMR"
BASE COUNT          520 a          714 c          691 g          521 t
ORIGIN      
        1 cagggttacc gtacaagagg cgctcgcgcg catcgcagcc gtgggttgat tctctgctct
       61 tactcctagg atctgaatgg cacagcaagc acgccgatca cgcccttccc gcatacagcg
      121 atctgagcgg ccatggcaga tggtcaagtc gctcgccaaa aaatcggtag acaaatggaa
      181 cgatgagccc ggcccccgtt tcgccgcggc gctcgccttc tacacagcgt tcaccatcgt
      241 gccgttcgtc gtgctggtcg tcatggtctc ggcgacgttc atgggagaag aggccatcaa
      301 aggagacttg cataaacacg tcggcgatct catcggcgag ccgtccgccg acggattgtt
      361 tcatttgatc gatcaatggc gggcggctgg atcgcctgtg ttgaatgaat tggtggcgct
      421 gggcgtcttc cttctcggga cggtccatgt catggatcag ctcagggacg cattggattg
      481 catttggggt ctgaagacca aacgaccacg ggcgatgatg gcacggctca agcagcgatt
      541 tgcctccgtg tccggcctct tgggcattgc tttcatcctg ctctcttcgt tgacgctcag
      601 cgcctggttg aacgtcggga ttcagtctac tttcggaggc atcggcggcc ccatgtggat
      661 gcagaaagcc atcggaaccg ccatgtcgtt tctcatcatc gctgcgctct tcgcccttat
      721 ctacaaatgg gtgccgcaag cgaaggtggc gtgggcggac gtctggatcg gcgcgctcat
      781 tacaggtgcg ctctatagcg tgggcagcat gttgatcgtg gtgaagctag gacacagtcc
      841 gctcgtgacg ttctatggag gggccggctc gctggtgctg gtattgttgt gggcgtacta
      901 cgcctcccaa atttttcttt tcggcgccgc ttttatcgcc gtctatgcca gccaatatgg
      961 ctcgcatgtc cgaccgaccg aaggtgctgt cgccgtccat tctgccgcca aaggcgagag
     1021 tgcggacgag gacgattgat actttatcga accctctgaa acacgttgat ggcgaacagg
     1081 acaatggccg agccaataac catggaagcg aggccgagaa acggatccag tccggtgttg
     1141 ccgcgcagag cgctgatcaa caatgccatt gccgcgagta aaccgacatt gtgcaaccag
     1201 aactggacag tcgccaaccg ggatgctcct gcatgaggaa attgatggta gatcaatccc
     1261 accagcgcca atgatgccca ccccaggaga ttgaaatggg catgaacagg aaccatgaca
     1321 tgatcacctg acgcacccat gtagatgcct aaccccactc ccgtgacgag gtagaccacc
     1381 gctgccttga tccacaagac gccgggactt cgcatcagag actcctcggt tgatggtaag
     1441 tagcgcgaga cgtcgtcaaa ccgaggcagg ataccccagc ggcagaattt tgcaactaca
     1501 ccgaccttac cgcatccgcg tcacacggaa atcgccttcg agcttccagg tatgtccatc
     1561 cggattgagg gcttcgccgg tccagtgaaa cgaatcgcgc gtgatctcgg agaacatcca
     1621 gcgagtgggg gtcccgtcgg gacgaacccc tacctgcaca acattcttac cgctccagcg
     1681 accgatctgc tcttcccggt ggtctcccgc tggattgatc caggtgatgc gccaagcctg
     1741 aatcgagggg tcccagactc tcaaggtcgt gccatacatg ttcattcgct tgtccagatt
     1801 cgcggtgcgg tccgccacgc agggcatgat ccacacatcc tgaatcgcac gaccttcgag
     1861 tgcccacccg aagtgtgcct ctcctttgag acgttgcgca cttacatccg tcgcccaata
     1921 gacacggatg tctaagttcc agctcccaac cagccatcca taggcatccg cggattccgg
     1981 aatttcaggc gagcggccag gagccgccaa caatgcgcta cgcgtgtcca ctctctcttc
     2041 ccgatcaacg gcaaattggt gtgtggccat ggtacacctc cggaaaacgt ttgcgttttg
     2101 tgaaacaccg actctggcgt atctaaaaaa tgttttgacc tgggaaagcg actagttgtc
     2161 ggggccggtt agccgaaagc tggtgaacac gaagaggact caggccaacg gagaaaaccg
     2221 ctgctcaaag gcgaagctcg gagcaggagg acagcggatt caaccgtctc agtctttacg
     2281 gtgatgtcgt gatgcggtcg atggcatccg catagatcac gggaatgccc tttgggtctt
     2341 tttcatccgc cacaacgacc ccacggatgc ggagatgttc gttctcgcga accggctgca
     2401 tggcatttgg ttgacaggtg ccggccgtcc caacccgaat ggtgcc
//
LOCUS       DASTQY010000057         3013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_763707, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000057 DASTQY010000000
VERSION     DASTQY010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763707"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2051
                     /locus_tag="VFR82_01845"
     CDS             <1..2051
                     /locus_tag="VFR82_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4500371.1"
                     /translation="ATQLGYQPDELIGESVLKVFDPDQHQAVLGQLTVCAASPMKVFQ
                     WEIQKVRKDGSTLWVKERAQAVMDHQGQILTLVVCEDITARRLTEDYMRESEERWRAL
                     FEHAGVGIAQLGLDGQFLRVNPRLCETLGYSSRTMTRCTFQQFVYPDDLQLILDQIRQ
                     LVSGKRPSFAMETRYRRSDDVWVWVEMTVSLVRDGACVPAYFIAVMQNIHDRKQAEAG
                     LARTTQLLQTLVRESPLPIVSLDLAARVTSWNQAATRLFGWSEEEVLGRELPYVPQEE
                     AARAESLWERGLRGEIHGPIELRRCRKDGKILDLLLWPVFVHSEGGDVSTAIGLYVDQ
                     SNLKRAEEALLASEERFSKAFRSSPYPIGITDLETGACLDINDAALEVFGFTREEVIG
                     QTTSVLGIWPDPEARQEFVERLRTKGTIRNLEVPLKSKRGEVRQFLASTEVIVLNGNE
                     CLVTIGTDITEQKRAEEALRRSEVAVRQAFEERERLSQDLHDNLLQSLYAVGMGLELT
                     KQRIQRISRTNAKRLEQSVGQLNNVIREVRSFISHVQPPVVFEQSVADTLRTLAGSFM
                     ATGAGEIVVTVDDEAGLRLAPEQRTHVLAIAKEALSNSIRHTKADKRMVTLRRHGNKI
                     RLEVIDNGHGFSPLQRRKQGMGMGLQNMRARARKLHGRISITSVLNQGTTVTLTIPST
                     "
     gene            2213..2767
                     /locus_tag="VFR82_01850"
     CDS             2213..2767
                     /locus_tag="VFR82_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HEU4500372.1"
                     /translation="MSDQRIGSTISENTNALEHGFEVVVFASRWIQAPLYGGLIVAEL
                     LYAYKFLVELWEMVVHVQGLEETKFMLGVLGLIDVTMVANLLTMVVIGGYATFVSKLS
                     LEGHPDRPDWLSHVDPGTIKVKLAASLIGISSIHLLKAFVDVANENPEHIKWKIFIHM
                     TFLGSAILLAWTDKIMQKDKKHSP"
BASE COUNT          676 a          852 c          865 g          620 t
ORIGIN      
        1 gcgccacgca gcttggatat caacccgatg agctgatcgg tgagtccgta ctgaaagtct
       61 ttgatccgga tcagcatcaa gccgtcctcg gtcaactcac cgtttgcgca gccagcccga
      121 tgaaagtttt tcagtgggaa atccaaaaag tgcggaaaga tggcagcacg ctgtgggtga
      181 aagagcgggc gcaggccgtc atggaccatc aagggcagat ccttaccctg gtcgtttgtg
      241 aggacatcac cgcccgccga ctcaccgaag actatatgcg ggagagcgaa gaacgctggc
      301 gggcgctctt cgaacatgcc ggcgttggca tcgcccaact cggtttggat gggcagttcc
      361 tcagagtcaa tcctcgcctc tgcgagacgc tggggtattc atcaagaacg atgacgcggt
      421 gcacgttcca gcagttcgtg tatcccgacg atctccaact gattctcgat cagatccgcc
      481 aattggtgag cggcaagcgt ccctcgttcg ccatggaaac acggtatcgc cgcagcgacg
      541 acgtctgggt ttgggtcgag atgaccgtct cactcgtccg cgacggcgct tgtgtccccg
      601 cctatttcat cgccgtcatg cagaacatcc acgatcggaa acaggcagag gccggcctgg
      661 ctagaacgac tcagttgctt caaacactcg tccgtgagtc tccccttccg atcgtcagcc
      721 tggatctcgc cgcccgtgtc accagttgga atcaagcggc aacacgcttg ttcggatggt
      781 cggaagagga ggtcttgggc cgcgaactgc cgtacgttcc tcaagaagaa gcggccaggg
      841 ctgagtcgct ttgggaacgc ggactgcgag gggagattca cggtccgatc gaactgcggc
      901 ggtgccgtaa ggacgggaag atactggacc tcttgctgtg gcccgtcttc gttcactcgg
      961 agggagggga cgtttcgacg gccattggtt tgtacgtcga ccaatccaac ctcaaacggg
     1021 ccgaggaagc cctcctcgcg agcgaggaac ggttcagcaa agcgtttcgc tcgagtccct
     1081 atccgatcgg cattacggac ttggaaaccg gcgcctgcct cgatatcaat gatgcggcat
     1141 tggaagtctt cgggtttaca cgcgaggagg tgatcggcca gacgacctcc gtcctgggta
     1201 tttggcctga tcccgaggca cggcaggaat ttgttgagcg cctccggacc aagggcacca
     1261 tcagaaacct cgaggttccg ctgaaatcga agcgtggaga ggtccgccag ttcctcgcat
     1321 ccacggaagt gatcgtgctc aacggcaacg aatgtctagt gaccatcgga actgacatta
     1381 ccgaacagaa acgggcggaa gaggcactgc gccgcagcga ggtggcggtg cggcaggcgt
     1441 ttgaggagag agagcggctc agtcaagatc ttcacgacaa tctcttgcag tctctctatg
     1501 ccgtcggcat gggactcgag ctcacaaaac aacgcataca gcggatctca cgaacaaacg
     1561 ccaaacggtt ggaacagtct gtcggccaat tgaacaatgt gattcgtgag gtgcgatcgt
     1621 ttatttctca cgtgcagcct cccgtcgttt tcgagcaatc cgtcgccgat acccttcgga
     1681 cgctggcggg atcgttcatg gcgacgggcg ccggagagat cgtcgtcacg gtagacgacg
     1741 aggccggcct gcgactggct ccggagcagc gcacccatgt gctggccatc gccaaggaag
     1801 cgctgagcaa cagcatccgt cataccaaag cggacaagcg gatggtgacc ctgcggcgac
     1861 atgggaataa gatccgtctg gaagtcatcg acaacggcca cgggttctct ccccttcaac
     1921 ggcggaagca gggtatgggc atgggtttgc agaacatgcg cgctcgagcg agaaagcttc
     1981 acggccgtat ctccattaca tccgtcctca accagggcac gacagtcacg cttaccattc
     2041 cttcgacata agccgactgc gggagcactc ttccgacgca cctcaatcat gatgttccca
     2101 gtaggttgcc gtcgttcatg cgaaatattt gacagagacc gtccagccct ctaggataca
     2161 gcccccatca ggtccttcct ccgttgaacg tattcgcaaa caccggagca tcatgtccga
     2221 tcaacgcatt ggttcaacca tctcagagaa taccaacgca ctcgagcacg gttttgaagt
     2281 ggtcgtgttt gccagccgct ggatccaagc cccgctgtac ggcggcctga tcgtagccga
     2341 actgctctac gcctataaat ttttagtgga actgtgggaa atggtggtgc acgtacaagg
     2401 ccttgaagaa accaaattca tgctcggcgt gctggggctg atcgacgtca cgatggtcgc
     2461 caatctgttg accatggtgg tgatcggggg ctacgccacc tttgtcagca agttaagcct
     2521 ggaaggccat ccggaccggc cggattggct cagtcatgtg gatcccggca cgattaaagt
     2581 caaattggcg gcctccctca tcggcatttc aagcatacac ttgctcaaag cgttcgtcga
     2641 tgtcgcgaac gagaatcctg agcacatcaa gtggaagatt ttcatccaca tgaccttttt
     2701 ggggtcggcc attctgctgg cttggaccga caagatcatg cagaaagaca aaaagcattc
     2761 accctaggct ctagcaagga tcccggctgt cgttcttgaa cgaggtgcct gtgacccgca
     2821 tagggctgct gtccggtcat ggttacccgt cggcctgctc cctgcaggct ctatcctatc
     2881 agtcctcgac gctctcatct tcaccaaggc atcattcaag acgaactcgt gaacaagctg
     2941 ccgaacggag aggcttttgt tgtttggaga tgaggcaaga gaacgaacaa ggaagggagg
     3001 cctcaaaaag tga
//
LOCUS       DASTQY010000058         2956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_161233, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000058 DASTQY010000000
VERSION     DASTQY010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161233"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(424..819)
                     /locus_tag="VFR82_01855"
     CDS             complement(424..819)
                     /locus_tag="VFR82_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500373.1"
                     /translation="MAYDVRVIPIREFMRTDITGEFDVSATRDLLSQLMAACKRENIT
                     RILIDCREASSQATVMEVYMLARDLAALGVTYEYRMAVLNRPKDEFDRGAFLEICATN
                     RGYQLKAFREFEAAFTWLTSEEPEVKVGA"
     gene            1107..1487
                     /locus_tag="VFR82_01860"
     CDS             1107..1487
                     /locus_tag="VFR82_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500374.1"
                     /translation="MAAIRTPPQHAELSVLIISCGLMLLLGEHSLAAETAPPPADTKQ
                     PQALEEFKPLTGLDNTPRGGTLPGGTLPGGTVPGGPLPGGTLPGGTLPGGTVPGGTVP
                     GGTVPGGTLPGGTLPGGTLAGGPL"
     gene            1547..1819
                     /locus_tag="VFR82_01865"
     CDS             1547..1819
                     /locus_tag="VFR82_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500375.1"
                     /translation="MKPIVIALTALLISAPLAWADEDKTEPRKGSVRVEDLPKPIPEL
                     LQKIQSLSKKIEPEISRLGSRLGEELTTTVKKLCDELQCQDKPEAK"
     gene            2081..2716
                     /locus_tag="VFR82_01870"
     CDS             2081..2716
                     /locus_tag="VFR82_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500376.1"
                     /translation="MHSLRHTCATIGLALLLGGCGGGGTIFALKDPTYAVKPTSVTVI
                     TGGSEESDLKLAEYLTKELKDRTKLQVVGQEEVSRAIPSYPIAVKTVEEKNGEVEWVD
                     PSEKTRLQKIQAKLKTHYVFVVWSKRLKQTSYCSSRGGCSNTYSADVYGNMLEYPSGQ
                     IVSHTAFNRRNSDSVLALFRSKGYYVDELLKKSAEDIVDEFIKVTNTGKSR"
     gene            2718..>2956
                     /locus_tag="VFR82_01875"
     CDS             2718..>2956
                     /locus_tag="VFR82_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500377.1"
                     /translation="MTRGRHSYLLPAIGLALIVSGCATFHLTQEPFQPKGKKLAVISG
                     LTNASSLGVAQALGEELGKTSRFHVLSQRQIAQAVP"
BASE COUNT          694 a          849 c          797 g          616 t
ORIGIN      
        1 ccgtcgtgat ggtcggatgg gcgcgatagt cgcaaggcgt ctccttcccc gtcttgccgc
       61 tcggcgccga agacgatttg gcgtccgcta tcgacccgga cagcagcata gtgatgagac
      121 cgacgaccca gaagcatgtg cggctctgcc ggcacgtcgt cacgtgtgtc atggttgtgt
      181 ctccttcctc tccttactga acatcgcctg taggaagtgt agcagaggag attcggctcc
      241 aaacctaaat gcgcggagtt gctgaagaaa tgaaaactta tctctatgag aaggcaggca
      301 gccctacgtg ttttctcata ccgtgcttgg ctcctctccc taagtttgaa ttcctgtcag
      361 atctttctgg ggaagaagcc ggttagagca ctgagcaggc gacgtccaat ccctcatgga
      421 gcattatgct ccaactttca cttcaggttc ttcggatgtc aaccatgtaa aagcggcctc
      481 gaactctcgg aaggctttga gctgatatcc ccggttggtg gcgcagattt cgagaaatgc
      541 acctcgatcg aactcgtctt tcggcctatt caataccgcc atacgatatt cgtacgtgac
      601 gccgagagca gccaaatccc ttgccaacat atagacctcc atcaccgtgg cttgggagct
      661 cgcttcacga cagtcgatga ggatgcgagt gatgttttcg cgcttacagg cagccatcag
      721 ctgactcaac agatcacgag ttgcactcac atcaaactca cccgtgatgt cagttcgcat
      781 gaattccctg atcggaatca cccgcacatc gtaggccatg tcgcaccctg ctggaccgtt
      841 ttcatcgacc gatgattgag agagtcggat taagagcttc tccgaagaaa agatcaaggg
      901 gtcttgttca ggagggagaa cggaatcgat tgtgagaagc tgactgaacg atccacagat
      961 cacgaaaaaa tgccgtcatg tgaccaccac cctgcatcac gagaccgttc acgatgttca
     1021 gggacggaac gactcgggac ttcccctgtg gctgttccaa tttgtgcggg ttacgctgac
     1081 atgtacgtga ggtctaagag atgcccatgg cggcgattcg aactccacct cagcatgccg
     1141 agctgtccgt tttgatcatt tcttgcgggc tcatgctcct gttaggggag cacagtctgg
     1201 cagctgagac cgcgccccca cctgcggaca ccaagcagcc tcaagcattg gaagaattta
     1261 aaccgcttac tggtcttgat aacactccgc gaggcggcac tcttcctggt ggcacgttgc
     1321 caggcggcac agtgccggga ggtcctctcc ctggtggcac tctgcctgga ggcacccttc
     1381 ccggcggcac agtgccgggc gggaccgtcc caggcggcac ggtcccaggg ggaacattac
     1441 caggcgggac gttacctggt ggcacgctgg caggcggacc actgtagctc tcagctaagg
     1501 gtgatgcggc aggtacccgc taatgatggc ctgacgggag cagatgatga aaccgatcgt
     1561 gatcgcgctg accgcgctcc taatctcagc accattggcg tgggctgatg aagacaagac
     1621 agaacctcga aaaggatctg ttcgggttga ggatctgcca aaacccattc ctgagctctt
     1681 gcagaagata caatcgttga gcaagaagat tgagccagaa atttcccgac tgggcagcag
     1741 gcttggagaa gaactcacta ctaccgtaaa aaagctctgc gatgaacttc agtgccagga
     1801 taagcctgag gccaagtagc gagaactccc cacccggtcc catcgctggc tccctgtggc
     1861 acccccaacc aaccgtcgca ctcccggtct ctcctcccgg agatggtttg gacgcgctgg
     1921 caattaaccc catctgactc acgcctagtc cattctcgta cacggtcgcc aatttgcctc
     1981 ccgccgctct gccaggtcca gccagccggg ttgacgacca tccacgtctt gctaagctta
     2041 aggcgacggt ttcctcaatc ccatcgattc ggaggacgcc atgcacagcc ttcgacacac
     2101 gtgtgccact attggactcg ctcttcttct cggcggctgc ggaggcggag ggacaatctt
     2161 tgcgttgaaa gatccgactt acgccgtgaa accaacatcc gtcaccgtca tcacaggcgg
     2221 atcggaagaa tctgacttga aactggccga gtatctcacg aaggaactca aggatcggac
     2281 caagcttcag gtcgtcggcc aggaggaagt cagccgagca atcccgagtt atccaatcgc
     2341 ggtcaaaacg gtggaagaga aaaacggcga agtcgaatgg gtggatccat ccgagaaaac
     2401 gcgtcttcaa aaaattcaag ccaagctaaa aacacactat gtgttcgtcg tctggagcaa
     2461 gcgactgaag caaacctcct actgctcaag tcgcggcgga tgttcaaata catactccgc
     2521 cgacgtgtac ggcaacatgc tcgaatatcc gagcggacaa atcgtatcgc acaccgcctt
     2581 caatcgacga aacagcgaca gcgtgctcgc cctcttccgg tccaagggtt actacgtcga
     2641 tgaactgctg aagaaatcgg cggaagacat cgtggatgag ttcatcaagg tcaccaatac
     2701 cggcaagagc aggtagcatg acacggggac ggcattcata cctcctgccg gcgatcggac
     2761 tggccctcat cgtaagcggc tgcgcgacct tccacctgac tcaggaaccg tttcaaccca
     2821 aaggcaagaa actggctgtg atatcagggc taaccaatgc cagttccctt ggcgtggccc
     2881 aggccttggg tgaggagctg gggaagacga gccggtttca cgtgctgtca cagaggcaga
     2941 tcgctcaggc tgttcc
//
LOCUS       DASTQY010000059         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_761170, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000059 DASTQY010000000
VERSION     DASTQY010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761170"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VFR82_01880"
     CDS             <1..963
                     /locus_tag="VFR82_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU4500378.1"
                     /translation="ESEFAVMSQCAGSAFGGARVFTTTAGPGTMRAMENFPMWAGARL
                     PIQCIVTCRGINSPLSIQPDTIEIAYLMNTGMLVWHAETAQDFYDWILKGYMVSEEPD
                     VHLPLALCCDGFFVTHTKDTVDLTPTDMCLPPYDPYRSPVPCMDMECPPVRMMRDPFV
                     MKSNYISYATHASWQQEIWAAAERSRKHTIAWLDGLIDTENIDAEVLIVTSGTAVSQG
                     REAIRLLADEGIRVGLVKIKTLRPWPGEELLEATKHAKHIIVPEFNVIGWMAREIKAT
                     IPRPERVIAGPRVCGGMTMPPEIIVEEIKKAIGVRSEVLAGRGS"
     gene            1045..1935
                     /locus_tag="VFR82_01885"
     CDS             1045..1935
                     /locus_tag="VFR82_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU4500379.1"
                     /translation="MSLNYVKFSPGFEAYMPKEYRDMVEHGPFGKKTTVSQMGSFKEI
                     LEEHPMCAGCAMTLFIRLAIVAFPNPEDTITVGTAGCGRLAISQAAIPFVYGNYGDQN
                     GVASGLSRGLRIRFGDQPKDVVVMAGDGGTADIGFQQVLHSWFRKERFTTIMLDNEVY
                     GNTGGQESGMTTRGAVLKMAPLGKKFEKMDMVAMAKIAGCSYIATVVPNNPRRVESCI
                     KKAVLIAREVGPTYIQAYTSCNIEYAIPTDQVMADAKTVEDDRYKFAEYVSDEAREYL
                     AARYGYKEFNQKPAAAIGTT"
     gene            2007..2384
                     /gene="iscU"
                     /locus_tag="VFR82_01890"
     CDS             2007..2384
                     /gene="iscU"
                     /locus_tag="VFR82_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEU4500380.1"
                     /translation="MAYSEKVVDHFNNPRNMGSFKKDEPGVGTGIVGAPECGDVMKLQ
                     IKVEAGTIVDAKFKTFGCGSAIASSSLSTEWLKGKTIQEAGKIKNTDIVQELNLPPVK
                     IHCSVLAEDAIKMALADYGKQAM"
     gene            2387..2842
                     /locus_tag="VFR82_01895"
     CDS             2387..2842
                     /locus_tag="VFR82_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500381.1"
                     /translation="MQTLDIHRPEMPELQFVLLVTALCTSRLTALNIPYALRTILFDR
                     CWVLIHDSPPPRRLEARVLDLRPWTEIAVEAMAETIRAGLTEAGIHTLAWDHPPSDPT
                     QTSTPDAQIVIDRPTLHASQGREPASEEAVDETGNLPDEEGIGGAASAR"
BASE COUNT          701 a          816 c          907 g          546 t
ORIGIN      
        1 gagagtgaat ttgcggtcat gtcgcaatgc gccggctccg catttggcgg ggcccgcgtg
       61 ttcacgacaa ccgccggccc tggcaccatg cgcgccatgg agaatttccc gatgtgggcc
      121 ggcgcgcgat tgccgatcca atgcattgtc acttgccggg ggatcaattc acccctctcc
      181 atccaaccgg acacgatcga gatcgcttac ttgatgaaca cgggtatgct cgtctggcat
      241 gcggagaccg ctcaggactt ctacgattgg attctcaaag gctacatggt ctcggaagag
      301 ccggatgtgc atctgccttt ggccctctgt tgcgatggat tcttcgtgac ccatacgaaa
      361 gataccgtcg acctgactcc caccgacatg tgcctgccac cctacgatcc gtaccggtcg
      421 cccgtcccct gcatggacat ggaatgtccg ccggtccgca tgatgcgcga tccgttcgtc
      481 atgaagagca actacatcag ctatgccacg cacgcgagct ggcaacagga aatctgggct
      541 gcggcggaac ggtcgcggaa gcatacgatc gcctggctgg acggactcat cgacaccgaa
      601 aacatcgatg ccgaggtgct catcgtgacg tccggcaccg ctgtctccca aggacgtgaa
      661 gccatccgtc tgctggccga tgaaggaatt cgcgtggggt tggtgaagat caaaacccta
      721 cgcccttggc cgggagaaga acttctggaa gccaccaagc atgccaaaca tatcatcgtg
      781 ccggagttca atgtcatcgg gtggatggca cgggagatca aagcgacgat cccccgcccc
      841 gaacgagtca tcgccggacc acgcgtgtgt gggggcatga ccatgccgcc ggaaatcatc
      901 gtggaagaaa tcaagaaggc aatcggcgtc cgatccgagg tacttgccgg acgcggaagt
      961 tgaaacggcg gcgcgccagc agatgagaca aagaccattt tcatcggcgc gattgcgccg
     1021 agccacaaga gaggacggta tcaaatgagt ctcaattatg tgaagttttc accaggattt
     1081 gaagcgtaca tgccgaaaga ataccgcgac atggtggagc atgggccatt cggaaaaaaa
     1141 accacggttt cgcaaatggg aagtttcaag gaaattcttg aggaacaccc gatgtgcgcc
     1201 ggctgcgcca tgaccctgtt cattcggttg gccatcgtcg ccttcccgaa tcccgaagac
     1261 accattacgg tgggaacggc cggatgcggc cgattggcca tttcgcaggc agccattccc
     1321 ttcgtctacg gcaactatgg cgaccaaaac ggcgtcgcca gcggcttgtc gcgtggacta
     1381 aggatccggt tcggagacca gcccaaagat gtcgtcgtga tggcgggtga cggcggtacg
     1441 gcggacattg gatttcagca ggtgctccat tcctggttcc gaaaggagcg gttcacgacg
     1501 atcatgctgg ataacgaagt gtacgggaac accggcggac aagaaagcgg catgacgacc
     1561 agaggtgcgg tgctcaagat ggctccgctt ggtaagaagt tcgagaagat ggatatggtg
     1621 gccatggcta aaatcgccgg ctgctcctat atcgccacag tcgtgcccaa taacccgcgg
     1681 cgggtggaga gctgcattaa gaaggcggtg ctgatcgccc gagaggtcgg tccgacctat
     1741 atccaagcct atacgtcctg caatatcgaa tacgcgattc cgaccgacca ggtgatggcg
     1801 gatgccaaga ccgtcgagga tgatcgctac aaattcgccg aatatgtcag cgatgaggcc
     1861 agggaatatc tggcggcacg gtacggatac aaggaattta accagaaacc tgcggcggcg
     1921 atcggaacga cttgagccga cacaaggcgg gatgcgctca gctcagcatg agtgagttcc
     1981 acgctgagac tttgaggagg gtaaacatgg catacagcga aaaagtggtg gaccatttca
     2041 acaacccacg caacatgggc agcttcaaga aagacgagcc tggcgtgggc acgggaatcg
     2101 tcggagcgcc cgagtgcggc gatgttatga agcttcagat caaggtggag gccggcacga
     2161 tcgtggatgc gaagtttaaa accttcggct gcggatcggc catcgccagc tcgagtctct
     2221 cgacggaatg gctcaagggc aagacgatcc aagaagccgg caagatcaag aataccgaca
     2281 ttgtgcagga actgaatctg ccgcccgtca agatccattg ctcggttttg gcggaggatg
     2341 cgatcaaaat ggccttggcg gattatggga agcaggctat gtaaccatgc agacgctcga
     2401 catccatcgt cccgagatgc ccgaacttca gttcgtgctg ttggtaacgg cactctgcac
     2461 ctcacgactg accgcgctca acatacccta cgcactccgc accatcctgt ttgaccgttg
     2521 ttgggtcctc attcacgaca gcccgcctcc ccgacggctg gaggcgcggg tgttggactt
     2581 gcggccctgg acggaaatcg ccgtcgaggc gatggcagag accatccgtg cggggctgac
     2641 cgaggcaggg atccacaccc tggcctggga tcatccgccg agcgacccga cacagaccag
     2701 cacaccggac gcgcagattg tcatcgatcg acccacactt catgcgtcac aagggagaga
     2761 accagcgagc gaagaggcgg tcgatgaaac cgggaatttg ccggacgagg aaggcatcgg
     2821 aggtgcggcc agcgctcgat gagatggcgg acttgaatgg ccagcctggt gcggcgtcat
     2881 tgttgatagc tgaagagtga ctgagctcaa gaggcgagct tgtggagatt gatgagcagg
     2941 gccgaggacg ctcaaaagaa gtgagcgggc
//
LOCUS       DASTQY010000060         2330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1771732, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000060 DASTQY010000000
VERSION     DASTQY010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771732"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..902
                     /locus_tag="VFR82_01900"
     CDS             102..902
                     /locus_tag="VFR82_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4500382.1"
                     /translation="MQNSPSQVRTGNISPTISGPNKDKDVLVVSGLVASLAAAIGAFW
                     YFSSTDPRIGQDQLNSSQVTEVLKSNQPMAVSVPVPNMQTAAAAASRPGAIHTDLYFE
                     VGRKGMTDEARKELLEDAALLKGDENLGVLIQGYTDQQGSRGYNLKLGLQRAEAVKTE
                     LIHAGVAEHQIKVVSLGKDGALCIDSSDVCRRMNRRVHLEIRPIGREHMMPPAVAVTT
                     QADISPASADSGKETGDSGLLIDSLFPSDNASDGTHNGPSALEPASGS"
     gene            complement(1012..1431)
                     /locus_tag="VFR82_01905"
     CDS             complement(1012..1431)
                     /locus_tag="VFR82_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500383.1"
                     /translation="MEQWIPRDALMWVSVGSGIALLIGAIAVPWIVINLPRDSFSNPT
                     RPRWLDQQPLAIRLPLRLVKNLLACVLIVLGIAMLVLPGQGIFAIVLGVLLADIPGKL
                     KLQQRIVGRQKVMNSLNWLRRKFRRPPLERPSIQAAA"
     gene            complement(1564..2061)
                     /locus_tag="VFR82_01910"
     CDS             complement(1564..2061)
                     /locus_tag="VFR82_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4500384.1"
                     /translation="MAAAQKIRVLIVDDHAMVRQGLRAVLQSYPNIEVVGEAANGEEG
                     VSSVQKLQPTAIVMDIGMPNLDGIGATRMIKMQYPQIAVVGLSVNAPSFHVDAMLKAG
                     AFEVLTKEKAVDELYSAIQRAIAAVQPILILEEKPATTGSAVETKRTEERAPSDVTTG
                     KEHKR"
BASE COUNT          548 a          658 c          648 g          476 t
ORIGIN      
        1 cccaggggaa aaaggaggtg atcacgggca caacttcacc ctcaggctca caaccaataa
       61 accttgaaca atacaatcat catgcatcga aggaggatcc tatgcagaac agtccatctc
      121 aggtcagaac gggcaacata agcccaacca tttcaggacc caacaaagac aaggatgtcc
      181 tggtcgtctc aggactcgtc gcgagcctgg ctgccgcgat cggcgccttt tggtacttct
      241 cttcaaccga cccccgtatt ggtcaggatc agttgaacag ttcgcaggtc acggaggtgc
      301 tcaagtctaa tcaaccgatg gccgtgtccg tgccggtacc caacatgcaa accgccgccg
      361 ctgctgcgtc tcgaccgggc gcgatccata ccgatctcta cttcgaagtg ggccggaaag
      421 gcatgaccga cgaagccagg aaagagctac tcgaagacgc agcgcttctc aaaggcgatg
      481 agaatctagg ggtgttgatc caagggtata cggatcagca gggctccaga ggatataacc
      541 tcaagcttgg tcttcagcgg gcagaagccg tcaagacaga gctgatccat gccggtgtgg
      601 ccgaacatca aatcaaggtg gtgagcctgg gcaaggacgg tgcactctgc atcgacagca
      661 gtgacgtctg ccggcggatg aatcgccgcg tgcatcttga aatccgtccg atcggccgcg
      721 agcacatgat gccaccggcc gtcgctgtga caacgcaggc ggatatctcg ccggcatccg
      781 cggattcggg caaggaaacc ggcgactcgg gcttgctcat cgacagtctg tttccttctg
      841 acaacgcctc tgatggcact cacaacggcc cttccgctct cgaaccggca tccgggagct
      901 gagtctggca gcgttgcgtc tgcacgacgt cgctcgtgca gacgtagctg cacgccaccc
      961 agccgatcac gcaggggcaa caagagactc gcgaagatgg tcagccggac atcacgcggc
     1021 cgcttggatg gatggtcttt caagcggtgg acgccgaaat ttgcgtctaa gccagttgag
     1081 gctgttcatc actttctgac gaccgacaat tcgctgttga agtttcagtt tcccgggaat
     1141 gtctgccaac aggacgccga gcacaatggc gaaaatccct tggccgggga ggacaagcat
     1201 ggcaatgccg agcacaatca ggacacaggc taacagattc ttgacgagac gaaggggaag
     1261 gcgaattgct aacggttgtt ggtcgagcca ccttgggcgg gttggattgg agaaggagtc
     1321 tcgaggcagg ttgatcacga tccagggaac ggctatggcg ccgatcaaca gggctattcc
     1381 cgatccaacg gagacccaca tcagcgcgtc tcgaggaatc cactgctcca tgcgtcctcc
     1441 ggacaacagc aaaccacttc ttatgtaacg tacgtaatga aagagtcatg aggtacgggg
     1501 gaaagccccg gacaggagcc cacgaacgac ttggcgcgct acatacggtg cgagaaaaac
     1561 ccctcagcgt ttatgttctt tgcccgtcgt cacgtccgaa ggcgctcgct cttccgtccg
     1621 cttcgtttca acagcagagc cggtcgtggc aggtttctcc tcgagaatca ggatgggttg
     1681 gacggcggct atcgcgcgct gaatggcact atacagttca tctacggctt tttccttcgt
     1741 caggacctcg aatgcgcctg ccttgagcat ggcgtccaca tgaaagctcg gagcgttgac
     1801 cgataacccc acgaccgcaa tctgggggta ttgcattttt atcattcggg tcgctccaat
     1861 gccgtcgagg ttgggcatgc caatgtccat cacgatggca gtcggctgga gcttttgtac
     1921 gctggacact ccctcttctc cattggcggc ttcgcccacg acctcaatat ttggataaga
     1981 ctgtaggacc gcccgcaatc cctggcgtac catggcatgg tcatcgacga taagaacccg
     2041 aatcttttgt gctgcggcca tttcagagaa tatcgatgtg aaaaaatgag tcgtcaaact
     2101 ttctggctcg gaaagccatc ataggctaga aatgtcgctc gtgtctgctg ttttctgtca
     2161 gggaaaacct acaaggacca tgtggctgat atcagccaac agaccgcaat gccggatcaa
     2221 aactgttaag cgcggtcggg gcgccaggtg caaattcgct tcaagttccg caatgtacgc
     2281 agggccttcc cctgaacatc tcattatctg accctaccaa gaatgctggg
//
LOCUS       DASTQY010000061        10205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_760314, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000061 DASTQY010000000
VERSION     DASTQY010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760314"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            698..1054
                     /locus_tag="VFR82_01915"
     CDS             698..1054
                     /locus_tag="VFR82_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500385.1"
                     /translation="MFKDFFQRFGSIKGVNIKQRLSVDQAPTWAQFLEHVGGPESPYY
                     RYVFHQESAIDLKRRVVTLFGGQGVKFEYSFDRYLNEAREVLTDCRAYQFFQLFDIYG
                     KGFASKIGWAGQPIHK"
     gene            1424..3016
                     /locus_tag="VFR82_01920"
     CDS             1424..3016
                     /locus_tag="VFR82_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4500386.1"
                     /translation="MRQQSGFSNWYRLVCAGAILLAFDHAAWAQLPNETYDFSITKGI
                     VEFGRGHYEQAAAFFEQARQAVPDDPEASEYLGQVLLRLKRFPEAEALFHELTIRHPT
                     RPQPWLGLAISQSQLGKYHEALGSLEEAQKLDPKNPLVYFYQGVISHELKSFNQSAAL
                     FSRAMALSPDLTPTVRYYTGMSYYQRGLLDQAQKEFEAAIGSGEPESEVVRTARAILE
                     QRTAPPKRAKQWDLNLSISGQYDSNVVLLPIGIQPPGGPTGISQKDDFRMTFYARGEY
                     RPIQNSVWTAGIAYGFYQSLNQTLTTFDIEDHAPSVFVQRQIGAVTARLQYAFDYVRV
                     GQDPFLFAHAVQPIITIAEGDSYFTQLQLRYQNKDFQDDRFAGNSFRNGNNWLAGVTQ
                     YAYFAQGAGHVRLGYTFDTDRTGGGSPSMATPGVQTSADWDYKAHRISVGLAVPEFWT
                     LRPSFAFDYYHLDYDNPSSFSSDGTTKRRDNVLFFTTTIGRPLTDWLLISAEYNYIRD
                     QSNLQVFDYNRSIFSVTLSSRF"
     gene            3284..4558
                     /locus_tag="VFR82_01925"
     CDS             3284..4558
                     /locus_tag="VFR82_01925"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEU4500387.1"
                     /translation="MSHQRLFFSLVAVILILVSSFATTSEGGTEAIGFYTAVSGQVTV
                     MHPTVDQVIPVKLNDEVLFKDVIQTQDHSRTKAFFQDDSILTVGESSRVEIDEYIYNP
                     EENVRRAIVKLVQGQVRALVSKVFKANGSRFEIHTPSAVAAARGTYFTVWVRNGQSGI
                     INIGEKGRVDFTSAGTTVAVDPGFFSVAQEGQAPSIPVPHFLGRNVDDRSGSTVEKAE
                     ATVPKVVRTHDRGQNLTKVELEPVSFPDGLAQALAAVELTTLREMPLLELPAQTLQAL
                     NIDALLVRALTTLTGLTTDPLGGSSLGTAIGDSGSHLTGALISVGTTTTSSGTVSVGS
                     LSVGSVSGVSTALVSPVVGTVAPVISTVVAPLAPVISPIVAPVAPVISPIVAPLAPVI
                     SPVVAPVAPVISPIVAPVAPVISPIISPLLSR"
     gene            4833..6878
                     /locus_tag="VFR82_01930"
     CDS             4833..6878
                     /locus_tag="VFR82_01930"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU4500388.1"
                     /translation="MGVALTIGLAFLSWLAPNVVVRLADTALDFQFRLRGERSPGQEV
                     ILVLVDEKSLKEIGRWPWSRNKQALLLDQIGVGKPRVIGLDIIYAEPESDNAHELKDW
                     LAAERRKRTLDGKALRLFEAHLRTMDPDRRFAQSLTEVGRVVIAVPFLVRETSADSLS
                     DGSHSLTETLRRSEFMLVKQTKSAEVPHPFEAARILPPLDIFAEQVKGFGHVYRLPDY
                     DGVTRREVLALRHGDAYYPSFALEVARLYLGLSREKMALMLGDGVQLGSAFVPADQKI
                     RMLINYSGRELRFPWVSATDVLHHRVPPEFFHDKVVLVGTAALGAYDQLSTPFSANFA
                     GTEKNATVIENILHQRFLTAGLWSGPIELTVVLFFGLALTSVLTRVRAFPGTCLTIGA
                     FISYIGTAQAMFVVQGLCLPIVMPTLTIGSVFIVTTVLNFIVKERQARDIHSMFASYV
                     SPRIVQELMKSPSKATIGGQRKELSMLFADLVGFTTFSEHRPAEAVVDQLNEYLSAMT
                     DVIFQWNGTLDKFVGDEIVVFWGAPLEQSDHAELAVQCAVEMRARLTVLQRQWKAQGR
                     PILENGIGINTGVALVGNIGADGKKMDYTMIGDHVNLAARVQELTRTLGHPLLLTEFT
                     AAKLKSRINPDSPQVGHMELRKLRTVTVKGRQEPVGVYTVACRPESVGVNSAITQVS"
     gene            complement(6905..8395)
                     /locus_tag="VFR82_01935"
     CDS             complement(6905..8395)
                     /locus_tag="VFR82_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU4500389.1"
                     /translation="MKDPTLQQFQDISALLNSSLDHGTIRERAIEAATLLMNAEAGSL
                     LLLDEETGDLYFDVAHSEKGEAVRQARLRGGQGIAGYVARTGKPVIVNDVQNDVRFFR
                     HVDQASGFVTRSMVCVPVTVHGRILGVLQAINQKSGEKFDESDLQNFVALGHQVGIAI
                     ENANLYGDIHRLFEGFISASVQAIESRDPTTSGHSQRVATLTCRLAEAVNRTTAGVYA
                     DTRFNADQLRELRYAAVLHDFGKIGVREQILVKARKLYPLQKELIKARFDFIKRTLEV
                     ETLQRKLEVYDADGEEKHALLTRLDQQLTQRIGEMDEMFSFILKCNERASQSPTHVEK
                     LKEVAHLEYRSFDGPRPFLTEEEFHALAVLQGNLTGAERREIERHVTHTFEFLSKIPW
                     TRALKGVPSIAWSHHEKLDGSGYPRRLSHEQIPLQARMMTICDIYDALTASDRPYKTA
                     ISSETALGYLQTQAKEGKLDRNLVEIFVKEHIYELTQPRSTTRKIA"
     gene            complement(8704..9486)
                     /locus_tag="VFR82_01940"
     CDS             complement(8704..9486)
                     /locus_tag="VFR82_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU4500390.1"
                     /translation="MRIAPRFLFSIATVGVVLMPALVHALDHDNLDPNRPIAIEDAYV
                     IPKGEIGVEGGVTFNERKEGKGRFGFQPQIIYGAFENMQLEIMTGFVTEPTTVVGDDK
                     SGDLSVGVLYNLNTETINLPAFAVRAEVGFPTGVNSKGVDTELTGVMTRSFGRWRTHV
                     NVGYTFLGSPQGQERSGTYRVVTAVSYPLGYPTSFRDTIIANVFTRQSDLVGQRNPTG
                     IGIGLRHQVSSRVVFDTGFGTEFLGPSDRSVFFSTMGLSIGF"
     gene            complement(9489..>10205)
                     /locus_tag="VFR82_01945"
     CDS             complement(9489..>10205)
                     /locus_tag="VFR82_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500391.1"
                     /translation="FIATRPTLNPNEEAIAPTYMKLAWKAQNTFDEAHALHRAAYDIY
                     VSDLKDNKRAIEKVLAFYRDSDYAITSKRLDHRRLDTLPYSKTFRRRFPLFNATIWAY
                     HYLQVVVYDALGHGENLSAKQKAVEPILKSYHGYLERPPVEWTFMPMTGELSPKFAEE
                     YPELANIFDNLHMMHDTISDILSSELFPTGEDKRKEIYRVAQNYYWASADATNPFIVR
                     DGQLHHESSTQTDVIEHSRH"
BASE COUNT         2355 a         2665 c         2685 g         2500 t
ORIGIN      
        1 aactgactag cgcacgttaa aagaagggtg cggcagccct ttccaatctt ccgttctcct
       61 cacctaaatt ctttgaccgg atttgtcagg tggtcggccc ctctgagagt tcttattctg
      121 tcgaggccaa tgaaggagat gccaacgatt gggcatcaga ccaataggca gcgatgcgaa
      181 agcgtctttg ggcctgagct cgatgagaag ttctcaacaa tgtgaatgtt atgcacactt
      241 atgatgaatt aagatgcaaa atggcaggaa gcggtgacaa aaaacgtcca aagataaata
      301 tgggggtcgg atgatctgga taatgggtct tcttatcttg ctgtcttttc cctcagtgac
      361 actgggtttt gagataagta acgccgcgtg gtccgactcg gtgtgggagg tccattgcaa
      421 agatatccga acgacttata acccgaacca tgccctcaga gtagatcgtc tcaccctcac
      481 tcagacagat cctcggttca gcgatttcct cttctggatc tgggccaaca aaattctaga
      541 acatcaggtg gaacaaattg gagggactga atggaccgga ccgacaaacg gggtcatttt
      601 tcattgggca cctggatcta ggtgggaaac tcacaccgtt gtttccctgg accatgcaat
      661 ccatcacatc gcggatgaac accagtggca agacgcaatg tttaaggatt tttttcagcg
      721 attcggctca atcaaagggg tcaacattaa acagcggctt tcagtggacc aagcgccgac
      781 atgggcacaa tttcttgaac atgtcggtgg accggagagc ccatactatc ggtatgtttt
      841 ccaccaagaa agtgcgattg atctcaagcg tcgggttgtg acgctgtttg gtgggcaagg
      901 ggtaaagttc gaatattcgt ttgatcggta tctcaatgag gcccgtgaag tactaacgga
      961 ctgtagggct tatcaattct ttcagttatt cgatatctat gggaaggggt ttgcctcaaa
     1021 gataggctgg gctggacaac ccattcacaa ataagaccga gacacactcg cctatttctt
     1081 tcctaccgat caagaacgtc gccaatggtc gttggccatc ggctcccgtt gaagttggtg
     1141 gtcccattgg gaagaaactc caggttcgcc acgagtgcac taaacaatac cggtatagct
     1201 cattcgatcc ttgctgaatg gctcggtccg ccgaaagtcc ttctgtctca attgcggagg
     1261 aaacggagga atgtagaaga gagcggaaaa gaacacatgc ggtacccata tgcgtggcaa
     1321 gattttttca attcgttgac tgatgcgtgt gctctgttac gctgcttaat acgtatcgct
     1381 tttcgaagtc gtacgcgtat tgcctccatc caagactatc ctcatgcgcc agcaaagtgg
     1441 attcagcaac tggtacaggc tggtgtgtgc gggcgccatc ttgctggcat tcgatcatgc
     1501 agcctgggca caattgccga atgaaaccta tgatttcagc atcacaaaag gcattgtcga
     1561 gtttgggcgg ggacattatg agcaggctgc tgctttcttc gagcaagcgc gacaggccgt
     1621 accggacgac ccggaagcga gcgaatacct gggacaggtc ctcttacgcc taaaaaggtt
     1681 tcccgaagcc gaagccctct ttcacgaact gacgatcagg catccgaccc gtccacagcc
     1741 ctggctgggg cttgccatct cacaaagtca actcgggaaa tatcacgagg cgctgggatc
     1801 gcttgaggaa gcccagaaac tcgatccgaa gaatcctctc gtttatttct atcagggagt
     1861 catctcccat gaattgaaat cgttcaatca gtccgctgcc ttgttttctc gggcaatggc
     1921 tctcagccct gatctcacgc cgaccgttcg gtactatacg gggatgtcat actatcagcg
     1981 cggattgctt gaccaggccc aaaaggaatt cgaagctgcg atcggctcgg gggaaccgga
     2041 atcggaagtg gttcgaacgg cccgagcgat tctcgagcag cgaactgcgc ctcctaaaag
     2101 agccaagcag tgggatctga acctaagcat cagcggtcag tacgacagca atgtcgtctt
     2161 acttcccatc ggaatccagc ctccgggtgg accgactggt atctcgcaga aagacgactt
     2221 tcgcatgacc ttctacgctc gaggagaata tcgaccgatc caaaactccg tgtggacggc
     2281 aggaattgca tacggctttt atcaaagtct caaccaaaca ctgacgacgt tcgatatcga
     2341 agaccacgca ccatcggtct ttgtccagcg ccagattggc gctgtcacgg cgcgccttca
     2401 atatgctttc gactacgtgc gcgtcggtca ggacccattt ttatttgctc atgccgttca
     2461 acccattatc acgattgcgg aaggcgacag ttatttcacc caactacagc ttcggtatca
     2521 gaacaaagac ttccaggatg atcgttttgc gggtaactcc tttaggaatg ggaacaactg
     2581 gctcgcgggc gttacacagt atgcctactt cgcgcagggg gcgggacatg tacggctagg
     2641 ttatacgttt gatactgatc gaacaggcgg cggatccccg tcgatggcca caccgggtgt
     2701 gcagaccagt gcggattggg actataaggc acatcggatc tcggtcggct tggctgtgcc
     2761 cgaattctgg acgctgagac cgagctttgc cttcgattac tatcacttgg attatgacaa
     2821 cccgagttcg ttttctagtg acggtacaac aaagcgccgc gataacgtcc tgttcttcac
     2881 caccacgatt ggccggcctc tcaccgattg gcttctcatc tcggcagagt ataactacat
     2941 tagggaccag tccaaccttc aggtattcga ctacaatcgc agtattttct ccgtcaccct
     3001 cagtagccgt ttctaaaaca ccattcttac ccgctagtca ggacacagac acaaattgtc
     3061 acccttggtg acgagatatg tcacccaact tattgaaaat acaacgaaaa tgttaaccct
     3121 ttcgaagggg gttccataat cgtgaatcgg ctgatgccct ccgttgtcaa tcagttgagg
     3181 atcacactcc tcatagagct gtctccggta cggttcctgc ccaacagtgg cagggagatt
     3241 tctgactggc tacgtatcct tcgttggagc ggagggatgt ccaatgagcc atcaacgact
     3301 ttttttcagc ctcgttgccg tgattttgat cctcgtctca tcgttcgcta cgacgtccga
     3361 aggaggcacg gaggcaatcg ggttctacac ggcggtttcc ggccaagtca ccgtcatgca
     3421 ccccactgtg gaccaagtga ttccggtcaa gctcaatgac gaggtgttgt ttaaggatgt
     3481 gattcagact caggaccatt cacgcacaaa agcgtttttc caagatgaca gtattcttac
     3541 ggtaggcgaa agcagtcgcg tggagatcga cgagtatatt tacaacccgg aagagaatgt
     3601 ccgcagggcg attgtgaagc ttgtgcaagg gcaggtgcgg gccttggtaa gcaaagtgtt
     3661 caaagccaat ggatcaagat tcgagatcca tactccctcg gccgttgctg ccgcgcgggg
     3721 aacctatttc accgtgtggg tgagaaatgg acagtctggg attatcaaca ttggtgagaa
     3781 aggacgcgtt gattttacgt cggcaggcac cacggtcgcg gtcgaccctg gattcttctc
     3841 agtcgcccag gaggggcagg caccatcaat tcccgtaccg catttcctgg gccggaatgt
     3901 cgatgaccgg tccggctcga cggtcgagaa ggctgaagcg acggtcccaa aagtcgtcag
     3961 gactcacgat cgaggtcaga atcttactaa ggtagagctg gaacctgtct cttttccaga
     4021 tgggcttgcc caggcattgg cagcggttga gctcacgaca ttgcgtgaga tgcctctcct
     4081 tgaattgcct gcgcaaaccc tccaggctct gaatattgat gctctcttgg tgagggctct
     4141 aacaacgttg actggtctaa ccacggatcc gcttggtgga agcagcctag gcacggcgat
     4201 tggcgattcc ggatcccatc taaccggggc tctgatttca gtgggaacga cgacaacttc
     4261 tagtgggacc gtgtccgtag gatctctttc cgtgggatcc gtttcagggg tatctacggc
     4321 gctggtctca cccgttgtgg gcacggtagc tccagtaatc tcaacggttg tggcgccctt
     4381 ggcgccggtg atctcaccga ttgtggcgcc cgtggctccc gtgatctcac caattgtagc
     4441 gcccttggcc cctgtgatct cgccggttgt ggctcccgtg gccccagtaa tctcaccaat
     4501 tgtagcgccc gtggctccgg tgatctctcc catcatctca ccgcttttga gccgatgaat
     4561 gctcctgcga tcctggtgac ccctcccgcc gtcaatcgat ggtgtctgtt caaaacctct
     4621 tgaatgcggc actggctcat cgacgttggc tcaaccggcc aaggctgcgg ccgtgctgca
     4681 ccaggcgtac tctgcaaatt gtcttgtccc gccgatacaa ccaggcacaa tggtatttcg
     4741 tgagtctaga gggcccattg tgaacggtgg gtagcgctgt gatcgtatcc gaaaatgaca
     4801 attcgaagtg tagctctcgt gttggactga gtttgggggt agccctcacg atcgggctcg
     4861 cgtttctgtc atggctggcg cctaacgtcg tggtacgcct tgccgatacc gccttggatt
     4921 ttcagttccg gctccgtggt gaacgaagtc cgggtcagga agtaatcctg gtgctcgttg
     4981 atgagaaaag cctgaaggag atcggacgat ggccgtggtc ccgcaacaaa caagcgctcc
     5041 ttctcgatca aatcggcgtt ggtaagccaa gagtgatcgg cctggatatc atttatgccg
     5101 agccggaatc cgacaatgcg cacgagctca aggactggct ggccgcagaa cgaaggaaaa
     5161 gaacgctcga cggtaaagcg ctccgtctgt ttgaggctca tcttcgaacg atggatccgg
     5221 atcggcgctt tgcccagagc ctgacagagg tcggtagagt cgtaatagcg gtgccgtttc
     5281 ttgtgcgcga gacttcagcc gattcactga gcgatggttc acattcactt acagaaacgc
     5341 tcagacgaag cgaattcatg ctcgtcaagc agacgaagtc tgctgaggtt cctcatccct
     5401 ttgaggcggc tcgtatactt ccgccgctcg acatctttgc ggagcaggtc aaggggttcg
     5461 gacatgttta ccgattgccg gattacgacg gcgtcacacg ccgcgaagtg ctcgccctgc
     5521 gccacggaga tgcctattac ccttccttcg cactggaagt tgcacggctc tatctcgggc
     5581 tgagcaggga gaaaatggcg ctcatgcttg gtgatggcgt gcaactgggg tcggcgttcg
     5641 tgccagctga tcagaagatc cgtatgctca taaactattc ggggcgagag ctccggtttc
     5701 cgtgggtgtc tgcaaccgac gtgttgcacc accgagttcc acccgaattc tttcacgata
     5761 aagtcgtgct ggtaggaacg gctgcattgg gtgcatacga tcagctcagc accccattct
     5821 ccgccaattt tgctggaact gaaaagaatg ccaccgtgat tgagaatatt ttacatcaac
     5881 gattcttgac cgctggcctt tggtccggtc caatcgaact cactgtggtc ctcttcttcg
     5941 ggctcgcctt gacctccgtc ctgacgcgtg tgcgtgcctt tcctggcacc tgtctgacta
     6001 taggcgcctt catcagctac ataggtacgg cccaggcaat gtttgttgtc cagggattgt
     6061 gtctccccat tgtgatgcct acgctaacca ttggctccgt gtttatcgta acgaccgtgc
     6121 ttaactttat cgtgaaggaa cggcaggctc gtgatattca ctctatgttt gccagttatg
     6181 tcagtcctcg gatcgtgcag gaactcatga aatctccctc aaaggcaaca ataggaggac
     6241 agcgcaaaga actgagcatg ctattcgctg acttagtcgg atttacaacg ttcagcgagc
     6301 atcgtccggc agaagccgtc gtcgatcaac tcaatgaata tctcagcgcg atgactgacg
     6361 tgatttttca atggaacggg acattggata aattcgtggg ggatgagatc gttgtgtttt
     6421 ggggagcgcc cttggaacag tccgatcatg ccgagctggc cgtgcagtgt gcagtcgaaa
     6481 tgcgggctcg attgacagtt ttgcagagac agtggaaggc gcagggaaga ccaatcctcg
     6541 aaaacggaat cggcatcaat acaggcgtgg ccttggttgg caacatcgga gccgatggca
     6601 agaaaatgga ttacaccatg ataggtgacc atgtgaattt ggccgcacgc gtgcaagaat
     6661 taactcgaac actaggccat ccccttctct taaccgaatt cacggcagcg aaactgaagt
     6721 cacgaatcaa tccagacagc ccacaggtag gtcatatgga gctccgaaaa cttcggaccg
     6781 tcacagtcaa aggccggcag gaaccggtcg gagtctacac tgtggcatgt cggccagaaa
     6841 gtgttggagt taacagcgcc ataacccaag taagttaggg gaggccgaaa gccaaaacca
     6901 aggatcaggc aattttgcgg gtcgtcgagc gaggctgagt gagttcgtag atgtgctctt
     6961 ttacaaagat ctccaccagg tttcgatcga gctttccctc tttcgcctgg gtctgaagat
     7021 agccaagcgc cgtttcagac gagatggcgg ttttgtacgg ccggtccgag gccgttaaag
     7081 cgtcgtagat atcacaaatc gtcatcatgc gagcttgcag cgggatctgc tcatgcgaga
     7141 gcctccgggg atagccgcta ccatccaatt tttcatgatg gctccacgcg attgagggta
     7201 cacctttgag cgctcgcgtc catggaattt ttgagagaaa ctcaaacgta tgagtgacat
     7261 gccgttcgat ctctcgccgt tcggcccctg tgagatttcc ttggagcacc gccaacgcat
     7321 ggaattcctc ttccgtcaga aagggacgag gtccatcaaa tgaccggtac tcaaggtgtg
     7381 cgacctcctt caatttttcc acatgtgtag gagattgcga tgccctctca ttgcatttta
     7441 ggatgaaaga gaacatctcg tccatttctc ctatccgttg agtcagttgc tgatccaagc
     7501 gtgtgagcaa cgcatgcttt tcctctccat cggcatcata cacctcgagc tttcgttgca
     7561 acgtctcaac ttccagagtt ctcttgatga aatcaaatcg cgctttgatg agttccttct
     7621 gaagcgggta cagcttccgt gctttgacaa gaatctgctc ccgaacacct atcttcccga
     7681 agtcgtggag caccgccgca taacgaagct ctcttagctg atccgcattg aaacgagtat
     7741 cggcatagac tcccgcagtg gtccgattga cggcttcagc gagtcgacac gtgagggttg
     7801 ccacacgctg gctatgccca ctggtggtag ggtctcggct ttcaatggct tgcacgctgg
     7861 cggaaatgaa cccttcgaat aggcggtgaa tatctccgta gagattggca ttctcgatgg
     7921 caatgccgac ctgatgtcca agggcgacaa aattttgcag atcgctttca tcgaactttt
     7981 ctccactctt ctgattgatt gcttggagca ctcccaaaat acgcccgtga actgtaacag
     8041 gcacacaaac catgctgcgc gtgacaaacc cgcttgcttg atccacatgc cgaaagaaac
     8101 ggacatcgtt ctggacatcg ttgacaatga ctggctttcc ggttcgagcg acgtatcccg
     8161 cgataccttg cccaccacgc aagcgggctt gccgaacagc ttcgcctttc tcgctatgcg
     8221 ctacatcgaa atacaagtcc ccagtttctt catccaacaa gagcagcgat ccggcttcag
     8281 cgttcatcag gagagtggcc gcttcaatgg cacgctcacg aatagtcccg tggtcgagac
     8341 ttgagttcag cagggcgctg atatcctgaa attgttgaag tgtagggtct ttcatggcat
     8401 gagatcgcat gcgtcggtgc gcaaagtgtc tcagtgaaac tgtaccatgg tagattcgat
     8461 ttgcacggaa aagctctgtt tgggaaacaa gggtatttga aatcttaatc atcgttatcc
     8521 tcatcttcta ccaggacagg acaacttaac cccatgtagc cccatgtcag gagagtccgg
     8581 ctttgtggct tcttgtgggg gcaggttgta atccgggtca ccggctccag aatttccaac
     8641 cgctttgttc cgctatgcga ttttgaaaag gcagcgaact aataaaaacg cagtcattca
     8701 cctttagaat ccgattgata ggcccatggt gctgaaaaac actgagcgat cagacggtcc
     8761 gaggaattcg gtaccgaatc cagtatcgaa cacgacacga gagctaactt ggtggcgtag
     8821 accgattcca attcctgttg ggttgcgttg ccctaccaaa tcggactgcc gcgtgaagac
     8881 gttggcgata atggtgtctc gaaagcttgt gggataaccg agcggatagc tgacagcggt
     8941 cacgacacgg taggtcccac ttcgttcctg cccttgcggc gatccgagaa acgtatagcc
     9001 aacattcaca tgcgtgcgcc aacgcccaaa tgaacgggtc atcacacccg tcagttctgt
     9061 atccactccc ttggagttga cgccggtagg aaaacctact tccgccctca cggcaaacgc
     9121 agggagattg atagtttctg tattcaagtt gtataggaca cctacactga gatcaccgga
     9181 tttatcatcg cccaccaccg ttgtcggctc agtcacgaaa ccggtcataa tttccagctg
     9241 catattctca aacgctccat aaatgatctg tggctggaat ccaaaccggc ccttgccttc
     9301 tttccgctca ttgaaggtca caccgccttc gactccgatt tctcctttcg gaatgacata
     9361 ggcatcttcg atcgcaatag gacgattggg atccaggttg tcatggtcca aggcgtgcac
     9421 cagcgctggc atcaggacga cgccaactgt ggcaatgctg aaaagaaagc gtggcgctat
     9481 tctcatcttt aatgtctcga atgttcgatt acatcggttt gagtggaact ctcatgatgc
     9541 agttgaccat ctcgaacgat gaaggggttg gtcgcatccg cactggccca atagtagttc
     9601 tgtgcgaccc tatagatttc cttgcgcttg tcttccccgg tgggaaagag ctcgctcgaa
     9661 aggatgtcac tgatggtgtc gtgcatcatg tgcaggttat cgaaaatgtt tgcgagctcc
     9721 ggatactctt ccgcgaactt cggactcaac tcgcctgtca tcggcataaa ggtccattcc
     9781 actggtggcc tttcgagata gccgtgatag ctctttagaa tcggctcgac cgccttttgc
     9841 ttggcggaca gattttctcc gtggccaagc gcgtcataca ccaccacctg cagatagtgg
     9901 tacgcccata tcgtcgcatt gaagagggga aaccttcgtc gaaacgtttt tgaatagggc
     9961 aatgtatcga gtcgccgatg gtctaagcgc tttgacgtaa tggcatagtc actgtctcgg
    10021 taaaaggcca agaccttctc tatcgcgcgt ttgttatcct ttagatctga cacgtaaatg
    10081 tcgtacgccg cccggtggag agcatgcgct tcatcgaagg tattttgggc cttccatgcg
    10141 agcttcatgt atgtaggtgc tatggcttct tcgttcggat tgagagtcgg tctcgtggct
    10201 ataaa
//
LOCUS       DASTQY010000062        15172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1366324, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000062 DASTQY010000000
VERSION     DASTQY010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 15172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15172
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366324"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..888)
                     /gene="fabG"
                     /locus_tag="VFR82_01950"
     CDS             complement(166..888)
                     /gene="fabG"
                     /locus_tag="VFR82_01950"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEU4500392.1"
                     /translation="MSRRALVTGGSGTIGAVICQRLAADGCTVIVHANRHPESAQQLA
                     EKIIADGGAARMSTFDIADPDATRKALEALLDEGPIQVLVNNAGVHDDAPMAGMTIEQ
                     WREVLDVSLNGFFHVTQPLLLPMIGTRWGRIVSISSLAGVLGNRGQANYAAAKSGLHG
                     ASKSLAIELASRGITVNVIAPGLIESPATRRSFTPEQVASIVPMKRAGTPEDVASLVS
                     FLVSEQAGYITGQVIGVNGGMA"
     gene            complement(885..1328)
                     /locus_tag="VFR82_01955"
     CDS             complement(885..1328)
                     /locus_tag="VFR82_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500393.1"
                     /translation="MTGMVKAEIGRLLPHGESMCLLDAVDSWDDSTITCRTSSHRDQR
                     NPLRVGGRLMITAGLEYAAQAMGVHVGLVNGGLRSGKQIGFVGSVRDVTFTTNRLDDL
                     TQDLTVTATRLLEGDSSYIYRFIVTHDGSALIEGRASIFIRAASS"
     gene            complement(1325..2116)
                     /locus_tag="VFR82_01960"
     CDS             complement(1325..2116)
                     /locus_tag="VFR82_01960"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEU4500394.1"
                     /translation="MKVGILGVGLLSAGLDGWMKGRAVLAGTSSYRPGSVPEPESSLL
                     PANERRRSSDAVRWALHVADEAVRQSALGAQDVATVFASSGGEMAVLDQLCRALTTAD
                     RALSPTLFHQSVHNTAAGYWGIATGCQESSTALSSYDDSSTLGLLEAVAYATVEDRPV
                     LFVAYDLPSPPPLDRARPIRSAFATALVVTPSAIEAPVRANLTLAAPSHAEVTKMEDQ
                     TLEQLRRGNPAARLLPLLRALAQRGSHRANLNVLDDQRLVVEIES"
     gene            complement(2116..3333)
                     /locus_tag="VFR82_01965"
     CDS             complement(2116..3333)
                     /locus_tag="VFR82_01965"
                     /inference="COORDINATES: protein motif:HMM:NF006618.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEU4500395.1"
                     /translation="MPPDRRSMTPLTLSAYTLVAANGRGIGAVSRSLRDRRSGLRPCD
                     FEDAYLKTFIGRVDGLEDFSLGNGLAPFDCRNNRLAWLALQQDGFMVAVAEAKQRYGS
                     DRIAVVMGTSTSGILETEHAYAQRDPQTGRLGDDFTARYRYTHNMFSSGHFVRTCLGL
                     RGPALVISTACSSSAKVFATAARYLQAGLCDAAIVGGVDSLCLTTLYGFSALQLLSAQ
                     PCRPCDEDRDGLSLGEAAGYALLESSANVGRKGAVALLGYGESTDGHHMSQPHPEGLG
                     AIRAIQQSLERAGLSPSDIDYVNLHGTATKANDLVEDKAVYHVFGSSPVVSSTKGWTG
                     HTLGAAGITEALMTAICLKEAFVPGTLHCERVDPALKSRVIRNNEARPIRLAVSNIFG
                     FGGNNCSLVLGVL"
     gene            complement(3320..5638)
                     /locus_tag="VFR82_01970"
     CDS             complement(3320..5638)
                     /locus_tag="VFR82_01970"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU4500396.1"
                     /translation="MRQRYGVGALWVLAMLLGLWLVVTRVVIHSELGDLLPGGTTATQ
                     RLLLTQLRSGQAGRMLLLAIEGGNPDQLADISNALREQLHATGHFVFIANGNQAVERR
                     DWDILFRWRYLLSPQVGHESFSSEWLRRTLEQRLEDLRSPLAPVIKPTLREDPTGEAL
                     AILAAVSPAETPAAHRGAWMSRDRSRALLVAETRATGFDADAQAKVQQHARALFAALV
                     GASPFRLHMSGPGVFAVEIARTIEMEAWWLSMAAATLVLVFLYASYRSVTLVLLSAIP
                     LTSGILAGMLAVQEWFGFIHGITLGFGIALLGVVDDYPIHFFSHLTARGSAGEVIRAI
                     WPTMRLGVLTTTIGFASLLLAGFPALTQLGVFAVVGILTGAAVTRWILPLFVPDGFVP
                     RYAWPGYESTLQRVSRMKPLVPVAVLLASAALLWSHTPIWETDLAELSPVTERQKQLD
                     HELRQELSAPDVRDVLMIEGPAEEAVLQAGETVLPKIEQLRRDGVIAGYDLISRYLPS
                     HRLQQQRQQSLPEPDVLRRNVETAVKGLPFTRGLFTPFIDAVEGARSQSPADRSLFEG
                     TALGMRMASLLFEQQGHWIGVVPLRSVEDRQRLKQIVTGWNVSQVGYVDLKEESNRLM
                     TDYRDRTFSVMLWGGLAIAVILAIAFKSLKLVVPVFLPILSALIVMVALVNASGESLS
                     LFHVATFLLVIGLGLDYALFFNRSEGSAVERAQTLYGLLVCATTTILVFGVLATSTIP
                     VLHAIGLTASVGSFCCLLFTGLMAKEKQHASG"
     gene            complement(5635..6261)
                     /locus_tag="VFR82_01975"
     CDS             complement(5635..6261)
                     /locus_tag="VFR82_01975"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-related protein"
                     /protein_id="HEU4500397.1"
                     /translation="MKAWPKWGRSQLRRQCALGCLAITLGSFPPMLQASDADWTIERL
                     MALVREQRKPSVAFEEATYSSVLTEPLKVRGVLRFTPPTTLEKLVTDPLHERHVIQGD
                     RVTFESQRTGVTRTLSLGEYPALRSFVEAFRMSLMGEAAQLRQLYEVTLEGTPAKWTL
                     LLRPLDPAGKSVVDYLLFTGSEGRIGTIAVRSPDGDRTVMTLLWGARR"
     gene            complement(6328..7224)
                     /locus_tag="VFR82_01980"
     CDS             complement(6328..7224)
                     /locus_tag="VFR82_01980"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500398.1"
                     /translation="MAVAWKTRQERGNRRLIRLMAWVAESLGRPVARALLIPVCVYYL
                     CRSGEVNQILRHYYLRAQGRAPGWIGLFHHYYWFGSTILDRVYFLRGRFGLFDIRVNG
                     IDTLTRALSLGRGCLLLGSHLGSFEVVRAMGVSREELDIKVLMDEQNAPLMRAFSHEM
                     NPALSGSVLQVGGIDNMLRVQECLEGGGIVGIMGDRLMSRDQAVRCNFLGSDAPFPTG
                     SIRLAHAVRTPVVMFFGLYRGTNRYDVHLELLSEEIRLSSERREDDIRRWTQQYADRL
                     AHYSRNATNNWFNFYDFWQDTP"
     gene            complement(7224..7547)
                     /locus_tag="VFR82_01985"
     CDS             complement(7224..7547)
                     /locus_tag="VFR82_01985"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500399.1"
                     /translation="MTYERTLTINSDHPALPGHFPGDPVVPGVIVLEEVIQALREIYG
                     QGLVVTGMPAVKLSSPLRPEERLTITVESQDTNTAAFTCEVGSRLVASGSIRFRLLGG
                     RQSGS"
     gene            complement(7537..8883)
                     /locus_tag="VFR82_01990"
     CDS             complement(7537..8883)
                     /locus_tag="VFR82_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU4500400.1"
                     /translation="MLDAPLIGCYRPDDLLAWESDGPRSASEFLSEVNNLAEVLPDRP
                     SVLNLLTSRYEFLVGFAAAILRRQVTLLPQSRAPDTLRRIAVDYDSCYCLTDRGEPVH
                     DIPAVVLPAERRLSRVSWKIPQIPLQQHVAIAFTSGSTGQPTPNLKTWDALVSVARAT
                     ARRLGLKAARVQGVVATVPHQHMFGLEASVMLPLQQGLTMHAGRPLFPEDIRAALTNS
                     PERSLLVTTPLHLRACLLAGVRLPPMGMILSATSPLTSDLAREAEQRFHAPVHEIFGF
                     AEAGSVASRRTVTDGDWRMLDGVALARNGAHGSIQAPHLPCPVPIPDRISVTGRATFR
                     VLGRVEDQVNIAGHRASLGDLTRQLLEVEGVHDGVFIVPEGKEGSLSRLMALVVAPGK
                     TQEDIQHALRRRLNPVFLPRPLLLLPRLPRNETGKLPREALRQFMERWKSESSHDV"
     gene            complement(8897..9169)
                     /locus_tag="VFR82_01995"
     CDS             complement(8897..9169)
                     /locus_tag="VFR82_01995"
                     /inference="COORDINATES: protein motif:HMM:NF006617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEU4500401.1"
                     /translation="MTSSPLSPFEREIAKLIVQALKLKADPSSIEPEAVLFGDGLGLD
                     SIDALELSLAIAQTYGYQLKSSDPDIKATFSSLRALARAIELHRTK"
     gene            complement(9166..9966)
                     /locus_tag="VFR82_02000"
     CDS             complement(9166..9966)
                     /locus_tag="VFR82_02000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4500402.1"
                     /translation="MNPESNHNTGTRSAGQLVPLPPHPLLRRYYDQDEARQRFLNDLF
                     NRTAHHYRNIDKATGLGSGLWYRRKALLRAGLTAGMHVLDVACGPGLVAQCARQIVGP
                     AGSVIGLDPSIGMLREARRGPCHTLVNAIGERLPFPDACFDFLSMGYALRHVSDLRDA
                     FSEYRRVLKPGGIVLLLDICRPRSPLLLSLSRFYVRTVLGIAFSASTGNRDMKTLMDY
                     WWDTTEHCVGADTILGALKEAGFLECALHELFNGLLRDYRAVKPKAVR"
     gene            complement(10040..10732)
                     /locus_tag="VFR82_02005"
     CDS             complement(10040..10732)
                     /locus_tag="VFR82_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4500403.1"
                     /translation="MRSVMHLASVPLVAALWSAVLVPQAQEIWAADDASPRTGPEIYG
                     GVYVFGSLAQNRNLNVGGEELPSTTVKNAAGGGFKAGVFPAFTGYVVGIQAESFGLGH
                     EVKAPASMGASGVQSGHGTLLAWTTMVSLIMQYPGKLVKPYGGVGAGWSSSYLVDAHL
                     TKGAVTQAGTLRDTSLAFQYFAGVRMLVTEKVFVFGEYKYFASRYQWSGNLEPSLDLR
                     THIVAIGVGLSF"
     gene            complement(10746..12746)
                     /locus_tag="VFR82_02010"
     CDS             complement(10746..12746)
                     /locus_tag="VFR82_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEU4500404.1"
                     /translation="MTQWQRILRWPKFHCTSKLVLWGSGLIGWNPRLLAFTASLFFLL
                     QVLGLDRGAAAPADLSYLPVLIEQATVARLADEREWHVLLHYNRNWFGGFTSMQDDPG
                     FFMAPNGKTDPQAELAATLARFFSDELVGRSKQPAQCAFVARYYWLKDKLHFDERRLP
                     AQPCERFTRWFNEFNAESISVIFPTGFMNNPSSMFGHTFLRIDGKGQTPQTRILDYTI
                     NFAAEVPPHAGPEYAVKGVFGGYPGHFSTIPYYLKVQEYRDIENRDIWEYRLALTELQ
                     VKRLLMHTWELGNAYFDYFFFDENCSYHILSLIEAAEPSIHLVDRFPLYAIPVDTVRV
                     LGQSGIIGEVVSRPSRSTLVRRKREAMTLDERVWLNRMIKDPAELKSDRFRSLSLDRQ
                     AFILDTASDYLLMKGSGGPEGEPYRQKNRALLSARSQMRVAPNELTIEPYVKRPDLGH
                     GTSRIGIGGGWRNDRVFEEINVRAAYQDLLDPDPGYTPNAQIEAMSFAVRHYHNESQA
                     RLERFSPLNMISLSPIDSLFQAPSWKLGLGMNTIAHRGCQLCTNGYLNGGIGGAVETH
                     LAGRQVFFAMAEVEANASKAYEESHRIGGGGTFGMLADITDRWKILASGTYLRYALGE
                     KSEDWRWSIGQRVSLTTNLAIRVEYNHRNHDNDVLATLHVYF"
     gene            complement(12694..13215)
                     /locus_tag="VFR82_02015"
     CDS             complement(12694..13215)
                     /locus_tag="VFR82_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HEU4500405.1"
                     /translation="MMKRVLVLSVAVLFGLQVGIALAAPTGNPDTGPGCGLGKLAWQN
                     YPHQKTIGVQVLEATTNGTFGSGTFGISSGTSGCTNDGQVWASEKANVFAAINFQNLA
                     QDMAQGHGEHLSSLATLMGIPADQQPAFFAMTQERYASFVKAGESSPGAMIKALNDAV
                     ATHPTLAKVSLHK"
     gene            complement(13455..13970)
                     /locus_tag="VFR82_02020"
     CDS             complement(13455..13970)
                     /locus_tag="VFR82_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU4500406.1"
                     /translation="MKRRTLRIGVIADTHGLYDPAVEPHFASVSEILHAGDIGGKDVI
                     QCLRRLAPVTAVSGNVDGYERSGWARRVVLRRAGMTIVLCHVLYENGRMNSEAQAWLD
                     LEQPDVCIFGHSHRPTIDRRGRTILFNPGSAGPKRFSLPRGVGILTISGGKAVPSLIR
                     LEARALKRGAC"
     gene            complement(13967..15115)
                     /locus_tag="VFR82_02025"
     CDS             complement(13967..15115)
                     /locus_tag="VFR82_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU4500407.1"
                     /translation="MGSATAPTSPNALCFSDGLPSTGLPCHVEISAKGLTVRFDEGNG
                     LDELNLPFRLLSVSAGGLNHDQLVVRWGTAPSTRTVYLKDPSLITTFRSAAPPELMKE
                     LDVTAATVRRTRSRNRTAWGIAVATIGALMVGIWLSMDLLVGWAVDRIPLDWERRIGE
                     SAFEDFLIQHPAVKDGAAVAAVQEISERLSDHIPDNPYIFKIAVVESEVVNAFALPGG
                     YVVVFTALLKEAENGEEVAGVLSHELNHVLERHALQRIVKQLGLVAVVGIIVGDQQGL
                     ARIMKQIGVELLSLKFGRNQETEADLTGLQLLHRSRIDPSGMVTFFQRLTKKERGGVE
                     WLSTHPMSAGRAQRLEAEIARLPRIPAEPFTFKWNTVQDALRPVAVRG"
BASE COUNT         3360 a         4590 c         4276 g         2946 t
ORIGIN      
        1 caatgacctg tgcacattgt tggcgcgatc gcttagccac ttcccgtacc gtcgcggctt
       61 cgttgtatgc cggaatgacg acccaacagc gatcgcgttc gcagccgcgt atggcgctgc
      121 cttctgtcgg tcccagggcg actggctctg aatggtcctg ccggttcacg ccatgccccc
      181 attgaccccg atcacttgtc cggtaatata tccggcttgc tccgagacca agaacgagac
      241 cagtgacgcg acgtcttcag gagtgcccgc ccgcttcatc ggcacgatcg acgcgacctg
      301 ctcgggagta aacgagcgtc ttgtggcggg tgattcgatc aagccgggag cgatcacatt
      361 gaccgtaatg ccccgcgaag ccagctcgat ggcaagcgac ttactggccc cgtgaaggcc
      421 ggattttgcc gcggcataat ttgcttgtcc gcggttcccc aggacccccg cgagcgacga
      481 aatcgacacg atccttcccc atctggttcc tatcattggc aggaggagcg gttgcgtcac
      541 atggaaaaac ccgttcaacg aaacatcgag cacttctctc cactgctcga tggtcatacc
      601 tgccatcggc gcatcgtcgt gcacgcccgc attgttgacg agcacctgaa tgggaccttc
      661 atccagaagc gcctcgagcg cctttcgcgt cgcatcggga tctgcgatat caaacgtcga
      721 cattcgcgct gccccgccgt cagcaatgat tttttccgcc aactgctggg ccgactccgg
      781 atgccggttg gcatgcacga tgactgtgca accatccgct gccagccgct gacagatcac
      841 ggcaccgatc gtcccgctgc ccccggtcac cagggcccgt ctactcatga cgacgccgct
      901 ctgataaaaa tcgacgcgcg cccttcgatc agggcggatc cgtcatgtgt cacaatgaag
      961 cgatagatgt aactgctgtc tccctccagg agacgggtgg cagtcactgt gaggtcctgc
     1021 gtcaagtcat ccagccgatt ggtggtaaag gtcacatccc gaacgcttcc gacaaagcca
     1081 atctgcttcc cgctccggag accgccgttt acgagtccga catggacccc catggcttgc
     1141 gcggcatatt cgaggcccgc cgtgatcatc aaccgtcccc cgacacgcaa cgggttccgt
     1201 tgatcgcggt gactggaagt ccggcaggta atggtactgt cgtcccacga atcgaccgca
     1261 tcgagcaagc acatagactc cccgtgcggg agcaacctgc caatctcggc tttgaccatc
     1321 cctgtcacga ctcgatttcc acaacaagtc gttgatcgtc gaggacattc aaatttgcgc
     1381 gatgggaacc tcgctgtgca agcgcacgaa gcagaggcag caaccgagcg gcagggttgc
     1441 cacgccggag ctgttccaac gtctgatcct ccatcttggt gacctcggca tggctggggg
     1501 cagcgagtgt cagattggca cggactggag cctcgattgc cgaaggggtc acgaccagcg
     1561 cggtcgcgaa cgcagatcgg atcggccgtg ctcggtcgag cggtggcggc gacggcaggt
     1621 cataggcgac aaaaaggacc ggacggtctt cgaccgtcgc ataagcgacc gcttctaata
     1681 gtcccagggt agacgaatcg tcataggagg acagcgcggt ggaggactcc tgacagcctg
     1741 tcgcaatgcc ccagtagccg gccgccgtgt tgtgaaccga ctgatgaaag agcgtcggag
     1801 acaaggcccg gtcggctgtg gttaaggcgc gacacaactg atccaagaca gccatttcac
     1861 cgccggagga ggcaaatacg gtggcgacgt cctgtgcgcc aagtgctgat tggcggacag
     1921 cttcgtctgc gacatgcaga gcccagcgca cagcgtcgct gctgcggcga cgttcgttcg
     1981 ccggcagcaa tgatgactcc ggctcaggta ccgacccggg tcgataggaa ctggtgccgg
     2041 ccaacactgc acgtcccttc atccagccgt cgagaccggc actcagcaga cccacaccca
     2101 agattcccac cttcatcaca agactccgag caccaagctg caattgttgc cgccgaatcc
     2161 gaagatattg ctcaccgcca atcgaattgg gcgtgcttcg ttgttgcgga tgacgcggct
     2221 tttcaatgcc ggatcgacac gctcgcagtg aagcgtgccg ggaacaaatg cttccttcag
     2281 gcagatggcc gtcatcaacg cctcggtaat gcctgccgct cccagcgtat gccctgtcca
     2341 gcctttcgtc gagctgacca ctggagatga tccgaacacg tgatagaccg ctttgtcctc
     2401 caccaaatca ttcgcttttg tcgcggtccc atggaggttg acatagtcga tgtccgaggg
     2461 ggaaaggccc gcccgttcca gagattgctg aatcgctcta atggcgccca gcccttcggg
     2521 atgcggctga gacatatgat ggccatccgt gctttcgccg tagccgagca gggctacggc
     2581 tcccttcctc ccgacattcg cgcttgactc aagcagcgca tagccggccg cttcacccaa
     2641 agaaagcccg tcccgatctt catcacaagg gcggcagggc tgcgccgaca acaactgcaa
     2701 ggccgaaaag ccgtacaacg tcgtcaaaca gaggctgtcc acacctccga cgatggcggc
     2761 atcgcaaaga ccagcttgca gataccgggc ggcagttgcg aacactttgg cactggagga
     2821 acaggcagtc gatatgacca gggcgggccc acgcagccct aggcaggtcc gaacaaagtg
     2881 tcctgacgaa aacatgttgt gggtgtatcg gtagcgtgcc gtgaaatcgt ctccaagcct
     2941 gccggtttga ggatcacgtt gcgcataggc atgttccgtc tcgaggattc cggaggtgct
     3001 ggttcccatg acgacggcga tgcggtcaga accgtagcgt tgtttcgctt cggccaccgc
     3061 caccatgaat ccgtcttgtt gcagggcgag ccatgccagc cggttgttcc ggcaatcgaa
     3121 cggcgccaat ccgtttccaa gcgagaaatc ctcgagacca tccacccggc cgataaatgt
     3181 tttgagatag gcatcctcga aatcacacgg ccgcaaaccg gagcgccgat cacgcaacga
     3241 ccgggacacc gccccgatgc cgcgaccgtt ggcggccacc agcgtgtagg ccgacagcgt
     3301 caagggagtc atcgatcgtc tatccggagg catgctgttt ctcctttgcc atcagcccgg
     3361 tgaagagcag gcagcagaaa gatcccacag aggcggtcag acctatggca tgcagtaccg
     3421 gaatcgttga ggtggccaga acgccaaaaa ccagaattgt ggtggtcgcg caaacgagca
     3481 acccatacag ggtctgtgct cgctccactg cactcccctc cgaacgatta aaaaacagag
     3541 cataatcaag acccaggccg ataaccagca ggaaggtcgc gacatgaaac aaggagaggg
     3601 attcgcccga cgcattgacc aaggccacca tcacgattaa cgcactgaga atagggagga
     3661 acaccggcac taccagcttg agtgacttga atgcgatggc cagaatcacg gcaatcgcca
     3721 gccctcccca cagcatgact gagaaggtcc ggtcacgata atcggtcatc agtcggttgg
     3781 attcttcttt gagatcgaca tagccaactt gggaaacgtt ccaaccggtc acgatctgct
     3841 tcaaccgttg gcggtcctca acactgcgca acggaaccac ccctatccag tgtccctgtt
     3901 gttcgaacag gagggatgcc attctcattc cgagtgcggt tccctcgaat aatgacctgt
     3961 cggctggcga ttgagatcgt gcaccctcca ccgcatcgat gaaaggggta aacaagccgc
     4021 gcgtgaaggg gaggcctttg accgccgtct caacatttcg tcgcaggacg tctggctccg
     4081 gcagggactg ctgtcgctgc tgctgcagac gatgactcgg gaggtagcgt gaaatcaggt
     4141 catagccagc gatgacccca tcccgacgca gctgttctat cttgggcagc accgtttcgc
     4201 cggcttgaag caccgcctct tcggccggtc cctcgatcat caacacatcc cgaacatccg
     4261 gtgcactcag ctcctgccgt aattcatgat cgagctgttt ttgcctctcc gtgacagggc
     4321 tgagctcggc cagatccgtc tcccatatag gcgtatgaga ccacaacaac gcggcgctgg
     4381 caaggagaac ggcaaccggc acgagcggct tcattctcga cactcgttgc agcgtcgatt
     4441 cgtaccccgg ccaagcatat ctcggaacaa aaccgtctgg gacgaacaaa gggagaatcc
     4501 accgggtgac tgccgctccc gtcaagatac ccacgacggc aaacactccg agctgggtga
     4561 gtgcgggaaa cccggcaagc aacagcgagg caaatcctat ggtcgtcgtc agcacgccca
     4621 gccgcatcgt cggccaaatc gctcgaatga cctctccggc cgaacctcga gcggtcaaat
     4681 ggctgaagaa gtggatcgga taatcgtcca ctacaccgag aagcgcgatc ccgaaaccca
     4741 gcgtgattcc atgaataaac ccaaaccact cttgcacagc caacatccct gccaggattc
     4801 cggaagtcag tgggatggca cttaacaaca ccagcgtgac cgatcgataa ctggcatata
     4861 gaaacaccaa cacgagcgtg gcggcagcca tcgacagcca ccaggcttcc atttcaatcg
     4921 tgcgcgcgat ctcgaccgca aatacgcccg gtccactcat gtgcaatcgg aacggcgagg
     4981 ctcccaccaa cgccgcgaac aaggcgcgcg catgttgctg aacctttgct tgagcatcgg
     5041 catcaaatcc agtagcccgt gtctctgcga cgagcaaggc tctcgaccgg tcacgggaca
     5101 tccaggcacc gcgatgtgcg gcgggcgtct ccgctggaga cactgccgcg agtatggcca
     5161 gcgcctcgcc ggtaggatct tctcgtaacg tcggtttgat gactggcgcg aggggcgacc
     5221 ggagatcctc aagccgttgt tccagcgtac ggcgaagcca ttcgctcgag aaggactcat
     5281 gaccgacctg cggactgagg aggtaccgcc atcgaaacag gatgtcccag tcgcggcgct
     5341 ccacagcctg gttgccgtta gcgatgaata caaagtggcc ggtggcatga agctgctcac
     5401 gcaaagcgtt gctgatgtcc gccagctggt ccggatttcc gccctcaatg gcgagtaaga
     5461 gcatccggcc ggcctggccg cttcgcagct gcgtcaacaa gagacgctgg gtcgccgtcg
     5521 ttccaccggg cagcaaatcg cccaattcac tgtggatcac cacccttgtg acaaccagcc
     5581 agaggccaag gagcattgca agaacccaga gggccccgac tccatacctc tgcctcaccg
     5641 acgggctccc cagagcagtg tcatgaccgt acgatcacca tccggcgagc ggacggcaat
     5701 agtgccgatg cgaccctccg agccggtaaa gagaaggtag tcgacgacag atttgcctgc
     5761 aggatccagt ggccgcagca agagcgtcca tttggcagga gtcccctcga gggtgacttc
     5821 gtagagttgt cttaactgcg cagcctcgcc catcagactc atgcgaaacg cctcaacaaa
     5881 actgcggaga gccggatact ctccgagcga aagggtgcgt gtaactcctg tgcgttggct
     5941 ctcgaatgtg acgcggtctc cttggatgac atgccgctca tgaagcggat cggtgacgag
     6001 cttttccagc gtcgtcggtg gggtgaaccg caggacgccg cgaactttta acggttcagt
     6061 cagcacggat gaataggtgg cctcttcaaa tgcgaccgat ggctttcgct gctcccttac
     6121 cagggccatc aacctctcaa tcgtccagtc tgcgtcggag gcttggagca tcggagggaa
     6181 gctcccaagc gtgatcgcca ggcagccgag ggcacactgg cgacgtagtt ggcttctacc
     6241 ccacttcggc caggctttca taaagatctg ctccatgact ctgggcggtc atagggtggg
     6301 tgattcctcc gacaaggggg cacggactca aggcgtgtct tgccaaaagt catagaagtt
     6361 aaaccagttg ttagtagcat ttcggctata gtgcgccaga cggtccgcat actgttgagt
     6421 ccatcgccgt atgtcgtcct cacgccgctc tgaagagagt cgaatctcct cgctcagcag
     6481 ttccaagtgc acgtcgtagc gattagttcc gcggtataga ccgaaaaaca tgaccacggg
     6541 cgtgcggacc gcgtgagcta atcgaatgga ccccgtcggg aacggtgcgt cggaacccag
     6601 aaagttacag cggacggcct gatcccggga catgagtcgg tctcccatga tgccgacgat
     6661 ccctccccct tctagacact cctggacgcg cagcatgttg tcgatgcctc cgacctgcaa
     6721 gacactgccg gaaagggcag ggttcatctc gtgagagaat gcacgcatca atggtgcgtt
     6781 ctgctcgtcc atcagcacct taatatcgag ctcttcgcgc gagacgccca tcgctctcac
     6841 aacttcgaaa ctgcctagat gcgagcccag gaggaggcat ccgcgcccaa gcgacagtgc
     6901 acgagtaagt gtgtcaatgc cattcactcg gatatcgaat aacccgaaac gcccacggag
     6961 aaaatacacc cggtcgagaa tcgtagaccc gaaccagtag taatggtgga agagtcctat
     7021 ccagccaggt gcccggcctt gcgcgcggag gtaatagtgg cgcaaaatct gattcacctc
     7081 accggaacgg cagagataat agacacagac gggaatgagc agcgccctgg ccaccggtct
     7141 gcccagcgac tccgccaccc aagccatcaa gcggatcaac cgacggtttc cgcgctcctg
     7201 tcgcgttttc caggcaacgg ccatcaactg cccgattgcc ttccaccaag caatcgaaac
     7261 cggatggacc cggacgccac gaggcggctt cccacctcgc aggtgaaggc tgccgtattg
     7321 gtatcctgag attcgacggt aatggtgagt cgttcttccg gtctgagtgg agaagagagt
     7381 ttgaccgccg gcattcccgt gacgactaac ccctgcccat agatttctct taacgcttga
     7441 atgacttcct cgagcacgat gacgcctggg acgaccggat cgcccgggaa atgacccggc
     7501 agcgccggat gatcgctgtt gatcgtcagt gtccgttcat acgtcatgcg acgattcgct
     7561 cttccaccgc tccatgaact gacgcaacgc ttcacgcggc agcttgcccg tttcgttgcg
     7621 aggaagtcgc ggcaaaagca gcaaaggacg cggcaaaaac accggattca gacgccgtct
     7681 cagcgcatgc tggatgtctt cttgggtctt gccaggcgcc acgaccaatg ccatgagacg
     7741 gctcaacgat ccttccttcc cctccggcac gataaacacc ccgtcgtgca ccccttcaac
     7801 ctccagcaac tgtcgggtaa gatcgcccag tgatgcacga tgtcccgcga tgtttacctg
     7861 atcctcgaca cggcccaaca cacgaaatgt cgcccgtccc gtcaccgaaa tgcgatcagg
     7921 aatcggaacg gggcacggca gatggggggc ctgtatggag ccatgagcgc cgttgcgtgc
     7981 cagggcaacg ccatcgagca tccgccaatc tccgtccgtc accgtcctcc gcgacgcgac
     8041 gctgcctgct tccgcgaacc cgaagatttc atgcacgggc gcatgaaacc gctgctcggc
     8101 ttcccgagcc agatcggagg ttaggggcga cgtcgctgac aggatcatcc ccataggcgg
     8161 aagcctcacg ccggccaaca gacaggcgcg gagatgcaag ggagtggtaa cgagtaacga
     8221 tcgctctggt gaattggtca acgccgcgcg aatgtcctcg ggaaacagcg gccggcccgc
     8281 atgcatggtc agcccttgct gtaacggcag catcaccgac gcctctaatc cgaacatatg
     8341 ctgatgagga acggtggcga ccacgccttg gacgcgagcc gctttcaaac ccagccgcct
     8401 cgcagtcgcg cgtgccacgg acaccaatgc gtcccacgtt ttcaggttcg gggtcggttg
     8461 tccggtgctg ccggacgtga acgcaatggc aacgtgttgt tggagcggga tttgcggaat
     8521 tttccaggac actctggaca agcgtctctc ggctggcagc acgacagcag ggatgtcgtg
     8581 gaccggttcc ccccggtccg tcaagcaata gcagctatca taatcgacgg caatccggcg
     8641 cagggtatcg ggcgctcgac tctgcggcag gagcgtcact tgccggcgca agatcgcggc
     8701 agcaaagccc acaagaaact cgtagcggct cgtgagcaga ttgagcaccg acggccggtc
     8761 gggaagcacc tcggccagat tgttcacctc tgaaagaaac tcactcgcgg agcgagggcc
     8821 atcggactcc caggccagta ggtcatccgg gcggtaacag ccgatcagag gcgcatctag
     8881 catgacacca caggaatcac ttcgtgcgat gtaattcgat cgcgcgtgcg agcgctctca
     8941 aggaggaaaa cgtggccttg atgtcgggat cgctggattt aagttgatac ccgtaggttt
     9001 gcgcgatggc cagcgacagc tctaacgcat cgatggaatc aagccccagt ccgtcaccga
     9061 acagcaccgc ttccggctcg atggaagagg gatccgcctt caacttgagc gcctgcacga
     9121 tcaatttggc aatctcacgt tcaaaaggtg agagcggtga actggtcatc gaacggcttt
     9181 cggtttcacg gcacggtaat cgcgcaagag cccgttgaac agctcgtgta gcgcgcactc
     9241 gagaaatcca gcctctttca aggcaccgag aatggtgtct gccccgacac agtgctcagt
     9301 cgtatcccac caataatcca tgagcgtctt catgtcgcgg ttccccgttg aggctgaaaa
     9361 agcgatgccc aggaccgtcc tgacgtagaa tcgggagaga gagagtaaga gaggtgaacg
     9421 cggacgacaa atatcgagca ggagcacgat gcctcccggc tttaacaccc gtcgatactc
     9481 agaaaatgcg tcccggagat cggagacatg ccgcaaagca taccccatgc tcaagaaatc
     9541 aaagcaggcg tcaggaaagg gcagccgctc accaatggca ttcaccaacg tatgacaggg
     9601 acctcgcctc gcctcgcgaa gcattccaat actaggatca agtcctataa ccgacccggc
     9661 agggcccacg atttgacggg cacattgcgc aaccagaccg ggcccacacg cgacatcgag
     9721 cacgtgcatg ccggctgtga gtcccgccct cagcagcgct tttcgtcgat accagaggcc
     9781 ggaaccaaga ccggttgcct tgtcgatatt gcgatagtgg tgggccgtac gattgaacag
     9841 gtcgttgaga aagcgctgac gggcctcatc ttggtcgtag tagcggcgca aaagaggatg
     9901 gggaggcaga gggacgagct ggccggctga ccgcgtgccg gtattatgat tggattcggg
     9961 attcatgaaa aatctcggct ccggtcccat cttgtagcag gactctcgcg gacgggcaag
    10021 ggggcggtcg gacctctcat tagaacgaga gaccgactcc gatggctaca atgtgtgtcc
    10081 gtaaatccag cgagggctct aggtttccgc tccactggta acggctcgca aaatatttat
    10141 actctccaaa cacaaagacc ttctccgtca caagcatgcg gaccccggcg aaatattgaa
    10201 aggccagact ggtgtcacgt agggtgccgg cctgtgtcac cgcgcccttc gtaagatgtg
    10261 cgtcgaccag ataggatgac gaccagcccg cccccacgcc gccatatggt tttacgagct
    10321 tgccggggta ctgcatgatg aggctcacca tcgtcgtcca cgccaacagg gtcccatgtc
    10381 ccgattgcac gcccgaagcg cccatggacg caggggcttt cacctcatgg cccagtccga
    10441 acgactcggc ctgaatcccg accacatagc ccgtgaaggc aggaaatacc ccggccttga
    10501 acccgccacc ggcggcgttt ttgaccgtag tcgatggcag ttcttcgcca ccgacattca
    10561 gatttcgatt ttgagccaaa gagccgaaga catagactcc tccataaatt tccggtcccg
    10621 ttcttggaga agcatcgtct gcagcccaga tctcctgtgc ttggggcact aagaccgcgg
    10681 accatagagc cgcgacgaga ggaaccgaag cgagatgcat gacagatcgc acgacagcgg
    10741 ctcggtcaga aatagacgtg caaagtggcc aacacgtcat tatcgtggtt ccgatggttg
    10801 tactccacac gaatggccag attcgtggtc agggagacgc gctgtccgat tgaccatcgc
    10861 caatcctcgg atttttcacc aagggcatac cgaagatatg tgccggaagc caggatcttc
    10921 catcgatccg tgatatcggc caacatgccg aaggtgccgc caccgccgat ccgatgggat
    10981 tcctcatagg ccttactggc attcgcttcc acttcagcca tggcaaaaaa cacctgccgc
    11041 ccagcgagat gtgtttctac cgctccgccg atgccgccgt tcaagtagcc gttggtgcac
    11101 agctggcaac cacgatgggc gatcgtattc atgccgagac cgagcttcca ggagggcgct
    11161 tgaaacaggg aatcgatcgg agaaagggag atcatgttca gggggctgaa gcgctccagc
    11221 ctcgcttgtg attcattgtg ataatgccgc acagcaaacg acatcgcttc tatttgcgcg
    11281 ttcggtgtat acccggggtc gggatccaac agatcctggt aggcggcacg aacgttgatt
    11341 tcttcaaaca cccgatcatt tcgccaaccg cccccgatgc caatccggct ggtcccatgg
    11401 ccgagatccg gtcgtttgac atagggctca atcgtcaact cattcggagc caccctcatt
    11461 tggcttcgag ccgacaagag ggctcggttt ttttgccggt agggctcccc ctctggtcct
    11521 ccgctccctt tcatcaaaag atagtcagag gcagtatcaa ggatgaacgc ttgccggtcg
    11581 agagagaggc tgcgaaagcg atctgacttc agctcggcgg ggtctttaat cattcggttg
    11641 agccaaaccc gttcatcgag ggtcatcgcc tcacgcttgc gccgtaccaa cgtgctcctt
    11701 gatggccgag aaacgacttc gccgatgatg ccagattggc cgagaacccg gacggtgtcc
    11761 acgggaatcg cgtaaagtgg aaaccggtcc accaaatgga tcgagggctc ggcagcttca
    11821 atgagcgaca agatgtggta cgagcaattt tcatcgaaaa agaaataatc aaaataggca
    11881 ttccccagtt cccatgtatg catcaagagg cgctttacct gtaattcggt caaagccaag
    11941 cggtattccc aaatatcgcg gttttcaata tcgcggtatt cctggacctt caaataataa
    12001 gggatcgtcg agaagtgccc cgggtagccg ccaaacaccc ctttgacggc atactcgggt
    12061 ccggcgtggg gcggtacttc tgcggcaaaa ttgatggtgt aatccaagat tcgagtctgc
    12121 ggagtctgcc ccttgccatc gatccgtaag aacgtgtgac cgaacatcga ggaaggattg
    12181 ttcataaatc cggtggggaa gatgacgctg atcgattcgg cattgaactc gttgaaccag
    12241 cgggtgaacc gttcgcacgg ttgtgccggc aagcggcgtt cgtcaaagtg cagtttgtcc
    12301 ttgagccaat agtacctcgc gacgaaggcg cattgtgccg gctgcttcga gcggccgact
    12361 aattcatcag aaaaaaacct ggccagggtc gcagcgagtt cagcctgcgg gtccgtcttg
    12421 ccgttagggg ccatgaagaa gcctggatca tcctgcatgc tggtgaatcc gccgaaccag
    12481 tttcggttgt agtgaagcag gacatgccat tcccgttcgt cggcgagacg ggcaactgtg
    12541 gcttgctcaa ttagtaccgg gagataagag aggtccgccg gggctgcagc cccgcgatca
    12601 agcccgagga cctgtagaag aaagaacagg gacgccgtga aggcaagtaa tcgggggttc
    12661 cagcctatga gaccggaacc ccacagcacg agcttacttg tgcagtgaaa ctttggccag
    12721 cgtaggatgc gttgccactg cgtcattcaa cgccttgatc atcgcaccgg gtgaagattc
    12781 ccccgccttg acgaaggagg cgtatcgttc ctgcgtcatc gcgaagaacg ccggttgctg
    12841 atcagccgga atgcccatga gcgttgcaag tgagctgaga tgttccccat gtccttgcgc
    12901 catatcctga gcaagattct ggaagttgat tgcagcaaag acgttcgcct tttctgaagc
    12961 ccagacctgt ccgtcgttgg tgcagccgga ggtaccgctg ctgatcccga aggtgccgct
    13021 gccgaaggtg ccgttcgtgg tcgcttccag cacctgaacg ccgattgtct tttggtgcgg
    13081 atagttttgc caagccagct taccaaggcc gcagccgggt cccgtatcag gattgccggt
    13141 tggcgccgcc aacgcaatgc caacttgcaa gccgaatagc accgctaccg agagaaccag
    13201 cactctcttc atcataagtc ctccttattg aaagagttgt cgtaccgccg gtgcctacga
    13261 ttagtaggca gtacctaggc caagtatacc caaattcgga tatggatgtc tcacaaaatt
    13321 tcgcaggatc gctgtcagac gcagtctgag gttttatatt cgttggttcg gtcatgcgat
    13381 ctgtacgcat ccgcgtcccg tgtcttacca agcgatcgca ccacccgacg cattctcatt
    13441 gtggcgattt gcattcaaca cgctccgcgt ttcaaagccc gggcttccaa tcgaatcaat
    13501 gacggcaccg ctttcccgcc tgagatcgtc aggattccga cccctcgcgg caacgaaaat
    13561 cgtttggggc cggcggaccc gggattgaag aggatcgtgc ggccgcgccg atcgatcgtt
    13621 ggacggtgac tgtgaccgaa gatgcacaca tcgggttgtt caagatccag ccaggcctgc
    13681 gcctctgagt tcatccgacc gttttcatac aacacatggc agagcacgat ggtcatgccg
    13741 gcgcgtcgaa gcaccacacg gcgtgcccag ccgcttcttt catagccgtc cacattaccg
    13801 gataccgcag tcaccggggc aagccggcgc aagcactgga tgacgtcctt cccgccaatg
    13861 tcgcccgcgt gaaggatttc gctcaccgag gcgaaatgtg gttccactgc cggatcgtaa
    13921 aggccgtgcg tgtcggcaat cactccaatc cgaagcgtcc gtcgtttcac ccccggactg
    13981 cgaccggcct gagcgcgtct tgaacggtgt tccatttaaa ggtaaaaggc tccgccggta
    14041 ttctcggcaa cctggcaatt tccgcctcga gccgttgggc ccggccggcg ctcatgggat
    14101 gggttgagag ccattccacc ccgcccctct cttttttagt gagccgctga aaaaacgtga
    14161 ccatcccgga gggatcgatg cgggaccgat gaagcaattg gagcccggtc agatcggctt
    14221 ccgtttcctg attgcggccg aatttcaagg agagcagctc gacgccaatc tgcttcatga
    14281 tcctcgccaa cccctgttga tcacctacaa taatgccgac cacggccacc aaccccaatt
    14341 gcttgacgat tcgctggaga gcatggcgtt cgagcacgtg gttcaattcg tggctcagca
    14401 ctcctgcgac ctcctcaccg ttttcggcct ctttcaaaag cgccgtgaac acgacgacat
    14461 atccgccggg cagggcgaag gcgttgacga cctcgctttc aaccacggca atcttgaaga
    14521 tgtagggatt gtcgggaatg tgatcactga gccgttcact gatttcctgc acagcggcca
    14581 ctgccgcgcc gtctttcact gccgggtgct ggatgaggaa atcttcaaat gccgattcgc
    14641 cgatccgtcg ctcccagtcg agcggaatcc ggtcgacggc ccacccgacg agcaggtcca
    14701 tgctgagcca tatgcccacc atcagggctc cgatggtcgc cactgcaatt ccccaggccg
    14761 tcctgttccg gcttctcgtt cgccggacag tcgcggctgt cacatcaagt tctttcatca
    14821 gttccggcgg ggccgccgac ctgaaggttg tgatgagaga cgggtctttg agatagaccg
    14881 tccgtgtgga gggagccgtc ccccatcgca cgaccaactg atcgtggttg agaccgccgg
    14941 ccgacactga aagaaggcga aacggcagat tgagttcgtc caggccgttg ccttcgtcaa
    15001 agcggaccgt caaccccttc gccgatattt cgacatggca cggcagcccc gtggagggaa
    15061 gaccgtcgct aaagcacagc gcattcggag aagtcggggc ggttgctgaa cccaagttat
    15121 tcgctcactg gaataaattg gcgcacccac gcgccaaagc taccgggatt ac
//
LOCUS       DASTQY010000063         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_156394, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000063 DASTQY010000000
VERSION     DASTQY010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156394"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /gene="atpG"
                     /locus_tag="VFR82_02030"
     CDS             complement(<1..835)
                     /gene="atpG"
                     /locus_tag="VFR82_02030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEU4500408.1"
                     /translation="MPSLQSLRRKIAAFKNTQKITKAMKMVAAAKLKRSQDRILAARP
                     YSHKMRAVLGNLSRRVNRSVHPLLQKRESRRIEVLVVTSDRGLCGGFNGSIVRKTTEF
                     LRQCEARGLQVSLSIIGRKGRDYFRRRSWKIRQEWAGVFDKLSFEHAIDIGGDLTEKF
                     IKGTFDELHVVYNEFKSAIQQRVIVEKLFPIDALAEFGAAPAEGMTGGSYLYEPDEAE
                     LLNVLIPKHFQVQAYRILLESAAAEHGARMAAMDGATRNAGQLIKKVTLYYNKTRQAA
                     IT"
     gene            complement(885..2402)
                     /gene="atpA"
                     /locus_tag="VFR82_02035"
     CDS             complement(885..2402)
                     /gene="atpA"
                     /locus_tag="VFR82_02035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU4500409.1"
                     /translation="MQIKAEEISAIIKEKIKGFDKQVDVKETGSVIQVGDGIAKVYGL
                     DGAMAGEMLEFPGGLYGIALNLEEDNVGAVLMGDDVGIKEGDPVKRTGRIAEIPVGEA
                     LIGRVVNAIGQPIDGKGPIKSQHSSRIEVVAPGVNTRQSVREPLQTGIKAIDAMIPIG
                     RGQRELIIGDRQTGKTAIAVDTIINQRGLGVFCIYVAVGQKRSTVARVVKTLEEHHAM
                     DYSMVVSATASDAAPMQFLAPFAGAAIGEYFRDHGKHALIVYDDLSKHAVAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDKLGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLGSDLFYSGIRPAINVGLSVSRVGGSAQIKTMKQVAGTLRLDLAQYREM
                     AAFAQFGSELDKATQMQLARGVRMVELLKQGQYKPMPVADQVISIYAGVNGFLDDVSV
                     DKVQQFEADLLHYAQQHHPDLKKEVASAGKIDEKLTAKLKEIITTFKQKMGYGAK"
BASE COUNT          525 a          794 c          737 g          638 t
ORIGIN      
        1 tggtgatcgc cgcttgtcgc gtcttgttgt aatacagcgt gactttcttg atcaactgac
       61 cggcattgcg cgtggcgcca tccatcgccg ccatgcgtgc gccatgctcg gccgcagccg
      121 attccaatag aattcggtat gcctgcacct gaaagtgttt cgggatgaga acattcaaca
      181 gctcagcctc gtccggctca taaaggtagc taccgcccgt cataccctcg gcgggtgccg
      241 ctccgaattc ggctagggcg tcgatgggaa acagcttctc cacgattacg cgctgttgga
      301 tggcggactt aaattcgttg tagacgacgt gcagctcgtc gaacgtgcct ttgatgaact
      361 tctcagtcag atctccgccg atatcgatgg catgttcaaa gctgagttta tcaaagacac
      421 cagcccattc ctgccggatc ttccaggagc ggcggcggaa atagtcccgg cccttccgtc
      481 cgatgatgct caaactgact tgaagcccgc gcgcttcgca ttgccgcagg aactccgtcg
      541 tttttcggac aatgctgcca ttgaaaccgc cacagagccc ccggtcgctt gtcacgacca
      601 acacctcaat ccttctgctc tcgcgcttct gtaagagggg gtgaaccgag cgattgaccc
      661 gccggctgag attgccgagc acggcacgca ttttatgcga gtaggggcgg gcagccaaaa
      721 tccgatcctg agatcgtttg agcttggctg cagccaccat cttcatggcc ttcgtgatct
      781 tttgcgtgtt cttgaaggcc gcaattttgc gcctcaagga ttgtaagctc ggcatgccgt
      841 gtctttcgtc tgggcaacag gaggaatgag ggggcttact acttttactt tgcgccgtac
      901 cccattttct gtttgaacgt tgtgatgatc tctttgagct tcgcagtcag tttctcatcg
      961 atttttccgg cgctggcgac ttcctttttc agatccggat ggtgttgctg cgcatagtgc
     1021 agtaggtccg cctcaaactg ctgcaccttg tcaaccgaca cgtcatccaa gaatccgttc
     1081 acgcccgcat agattgagat cacttgatcg gcgaccggca tcggcttgta ctggccctgc
     1141 ttcaacaact cgaccatgcg aacgccgcgg gcaagttgca tttgtgtcgc cttatccaac
     1201 tcgctgccga attgagcaaa agcggccatt tcacgatatt gggccaggtc caagcggaga
     1261 gtaccggcaa cttgcttcat tgtcttgatc tgagcggaac cgccgacacg cgacacagag
     1321 aggccgacgt tgatcgcggg gcgaatgccg gaataaaaga gatcgctgcc cagatagatc
     1381 tggccgtccg taatggagat gacattcgtc gggatatagg ccgagacgtc accggcctgc
     1441 gtttcgatga tgggcagcgc cgtaagactg ccgccaccca atttatcgct cagtttcgcc
     1501 gcccgctcga gcagccttga gtggagatag aagacgtcac ctggataggc ttctcgcccg
     1561 ggtggccttc gtaggagcag ggagagctgc cggtaggcca ccgcgtgctt agagagatca
     1621 tcgtagacaa tcaaggcatg cttaccgtgg tcccgaaaat actcaccgat cgcggcaccg
     1681 gcgaatggcg ccaggaattg catgggagcc gcatcactgg ccgtagccga gactaccatg
     1741 ctgtagtcca ttgcatgatg ctcttcgagg gttttcacca cgcgggccac cgtcgagcgt
     1801 ttctgaccga cggccacgta aatgcaaaat acaccgaggc ctctctggtt aatgatcgta
     1861 tcgacggcga tcgccgtctt gcccgtctga cgatccccga tgatcaactc gcgctggcct
     1921 cgaccgatgg gaatcatggc atcgatggcc ttgatgccag tctgtaaggg ctcgcgcacc
     1981 gattgtctgg tattcacacc cggagcgacg acctcgatgc gtgatgaatg ctgtgatttg
     2041 atcggcccct tgccgtcgat cggctggccg atcgcattga ctactcggcc gatcagggct
     2101 tcgccgacag ggatttccgc aatgcggccc gtccgcttga ccgggtcgcc ttctttgatt
     2161 ccgacgtcgt cgcccatcaa cacggcaccg acgttgtctt cttccaggtt caaggcaatg
     2221 ccatacaaac cgccgggaaa ttcaagcatt tcacctgcca tggcgccatc gagtccgtag
     2281 accttggcaa taccgtcgcc gacctggatc acggagccgg tttctttgac gtcaacctgc
     2341 ttgtcgaacc ctttgatctt ttccttgatg atcgcactaa tttcttctgc cttgatctgc
     2401 atggctactc cttgctcgtc gcaacggtca cagatgtcct cgtcttaagc tccggaacgc
     2461 gcgtcgcgcg aaagcggcag gtttggccgt ctttcgaggg aggctcgtgt cgtccttctt
     2521 agtctcgggt cagcaacgac tgcatggcgt tcaagcgtcc tcgaacggtg ctgtcgacga
     2581 cggtactgcc gatatgaatg cgcaggcctg caagatgcgc cgcatcagtg tgaaacgtga
     2641 cctcgacctc tcgcttcagc gtctcgcgca ggcgcccctt gatgcggtcc tgtt
//
LOCUS       DASTQY010000064         3726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1564820, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000064 DASTQY010000000
VERSION     DASTQY010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564820"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VFR82_02040"
     CDS             <1..905
                     /locus_tag="VFR82_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU4500410.1"
                     /translation="WSTAAEAAAEQDMTLIPRGEFTMGSHEHADEAAHQVVLDPYYID
                     KYEVSNARYKEFISAAGHPAPAYWDDPRLSKPNQPVVGVSWNDAAAFCKWESKRLPTE
                     AEWERAAKGPEGDNHYPWGHKLDSTKANYGQNVGRTMPVDSYPDGVNGFGVYNMAGNV
                     FEWVSDWYDPKYYKQSVALNPQGPEKGYNFANQGPVKVLRGGSWLAPETSLHTSHRFW
                     NQPENNSYGVGLGFRCAKSAPAVSEESIQAGREAFIQALIAMGAEKSSDATASIEKAL
                     AADPGNKEYLATRELIKKSMKKKN"
     gene            929..1303
                     /locus_tag="VFR82_02045"
     CDS             929..1303
                     /locus_tag="VFR82_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500411.1"
                     /translation="MVMTGSLNLMVVVTFSAALGAQYVWAGPINASHHPHPDQARAVH
                     EAEHAVDHAWEVYHRAALGGTIASPILQVEIEEHLHEARTLVAQAQEAAERGDHQRAE
                     RMVGEIHIHASHAIEGSKERKQ"
     gene            1300..2136
                     /locus_tag="VFR82_02050"
     CDS             1300..2136
                     /locus_tag="VFR82_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU4500412.1"
                     /translation="MTRGEGHVLNCWGVLSSLFLIAAADLPPVSEALQDRELDPVPMV
                     TIAAGEFLMGNPEGVGRADEWPQRRIYLDTFWIDEVEVTNERFMAFISTTGHQNPPNP
                     YGTGPLLSAKGIEQLPVVQVTWYDAKAYCSWAKKRLPTEAEWEKAARGTDGWKYPWGY
                     DLPTRQRANFDREWEDMKTHHPAGTLPGGDSPYGVKDMSGNAREWVQDWYDAEYYRRA
                     PNRNPKGPEKGIVRSIRGGSWHSPMSDITTSARGRGGFALQTHGTGFRCARGSESEAL
                     QQ"
     gene            2320..2559
                     /locus_tag="VFR82_02055"
     CDS             2320..2559
                     /locus_tag="VFR82_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500413.1"
                     /translation="MLVESYMDLQDDTGQIGIKALRCTGCGEVIDGVILKNRLQAPPN
                     LSYGTKRRKFAQRLGESSSRQPSDPTGDLEFGDKP"
     gene            2596..3516
                     /locus_tag="VFR82_02060"
     CDS             2596..3516
                     /locus_tag="VFR82_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEU4500414.1"
                     /translation="MDIVIAGGTGFIGKALCETLRRSGHRLTILTRRPATVEHLFGSQ
                     VTTVEWDALSAGTWERSLRDAQAVINLAGAGIADARWTDHRKRVLIESRLRPTRLLVE
                     ACARLSVKPQVFISASGVGFYGPQGTEVLDESSGRGRGFLSDLCVQWESAALEAASQG
                     IRVVCMRTGMVLEQDGGALPRIALPFRLFLGGPVMPGTQWISWIHRDDLVGLIEWALA
                     MPEVSGPVNGVAPSPVTMAEFCRLLGLVLRRPSWLPVPEFALRMALGQLGSIMTTGQR
                     VVPAVAERHGYRFRFREVQRALQAIFPAGA"
BASE COUNT          836 a          993 c         1183 g          714 t
ORIGIN      
        1 cgtggtccac ggcggctgaa gcggcagcgg agcaggacat gacgctcatc cctcgcggcg
       61 agttcacaat gggtagccat gaacatgctg acgaagcggc tcatcaagtg gtgctcgatc
      121 cttactatat cgacaaatac gaagtctcga acgcccgcta caaggagttc atcagcgctg
      181 cgggacatcc cgcgcccgcg tattgggatg atccgcggct gagcaagccc aatcaaccgg
      241 tggtgggggt gagctggaac gatgcggctg ccttctgcaa atgggaaagc aagcggttac
      301 cgaccgaagc agaatgggag cgggccgcca agggaccgga aggcgacaat cactacccct
      361 ggggacacaa gcttgattcc acgaaggcca attatgggca gaatgttgga cgcacgatgc
      421 cggtggattc ttatcccgac ggagtgaacg gcttcggcgt gtacaacatg gccggtaatg
      481 tcttcgagtg ggtgtctgat tggtacgatc cgaagtacta caagcagagt gtggcgctca
      541 atccgcaagg gccggagaag ggttacaact ttgccaacca agggccggtt aaagtcttgc
      601 gtggtgggtc ctggcttgcg ccagaaacat cgttacacac cagtcatcgg ttctggaacc
      661 agccggaaaa caactcgtac ggcgtcggac tgggattccg ctgcgccaag tcggctccag
      721 ccgtctcgga ggagtcgatt caggctggac gcgaggcgtt cattcaggcg ctgatcgcga
      781 tgggagcgga aaagagctct gatgccacgg cctcgatcga aaaggcgctc gccgccgatc
      841 cgggcaacaa agagtatctc gcgacccgcg aattgatcaa gaagagcatg aagaagaaaa
      901 attaacaccc gacaaccaca ggagaggcat ggtcatgaca ggctcattga atcttatggt
      961 cgtcgttacc ttcagcgcag cactgggagc ccaatacgtc tgggcggggc cgatcaatgc
     1021 gtcccaccac ccgcaccccg atcaggcccg tgcggttcat gaagcggaac atgcggtgga
     1081 ccatgcctgg gaggtgtatc accgcgcggc attgggcggg acgatcgcgt cgcccattct
     1141 tcaggtggaa atcgaggagc atctgcatga agccaggacc ctcgtcgccc aagcacagga
     1201 ggcagctgaa cgaggcgatc atcaacgggc ggagcgcatg gtcggggaaa tccacattca
     1261 tgcatcccac gccatcgagg gaagcaagga gcggaagcaa tgacacgagg cgagggccac
     1321 gtcctcaact gctggggcgt attgagcagc cttttcttaa tcgcagctgc cgacttgccg
     1381 ccggtatcgg aggcacttca ggacagagag ctcgatcccg tgcctatggt gaccattgcg
     1441 gcaggagaat ttctcatggg caatcccgaa ggagtaggtc gagccgatga atggcctcag
     1501 cgcaggattt atctggatac cttctggatt gatgaggtgg aagtgaccaa tgaacgattc
     1561 atggccttca tctcaaccac cgggcaccaa aacccgccga atccctatgg cactggacca
     1621 ctgctgtcag ccaagggcat cgagcagctg cctgttgtcc aggtcacctg gtacgacgcc
     1681 aaggcctatt gcagttgggc caaaaagcgt ctgccgacag aggccgaatg ggagaaggca
     1741 gctcggggaa cggatggttg gaaatatcct tggggctatg acctgcctac gcgtcaacgg
     1801 gcgaacttcg atcgggagtg ggaggacatg aagacgcacc atcccgcagg tacgttgccc
     1861 ggcggcgact cgccctacgg agtgaaagac atgtccggca acgccagaga atgggttcag
     1921 gactggtacg acgctgagta ctatcgacgt gcgccgaacc gcaatccgaa ggggccggag
     1981 aagggcatcg tgcgctcaat acgaggcgga tcgtggcaca gccctatgtc agacattacg
     2041 acgagtgctc gtggcagagg gggcttcgcg ctacagacac acggcaccgg attccgttgc
     2101 gcgcgaggga gcgaaagcga ggcgctacag caataatgtg gacggcctca gccgacgaaa
     2161 gtggcttgac ctccttaatg gagatgtcgc ttctctgcag ggcgggcgcc tactgctcga
     2221 tgaacaaaat gaatgaggtg tcccgtggaa cacaatggga gaccggagca gatgtcgatc
     2281 cctcagatgt tttgtcgctg cggacggtgt ggaggactga tgttggtgga atcctacatg
     2341 gatttgcagg acgataccgg tcagatcggc atcaaagcat tgcgctgcac tggatgcggc
     2401 gaggtcattg acggagttat tctaaagaat cgcctgcagg cgcctccgaa tctcagctac
     2461 ggcaccaagc gacggaagtt cgcccaacgt cttggtgaga gttcttcgcg tcagccctca
     2521 gacccaaccg gcgacctgga gttcggggac aaaccctgac tggttttgat aaacgggcag
     2581 taaggagaag agaccgtgga tattgtcatc gcaggcggta cgggtttcat cggcaaggcc
     2641 ttgtgcgaaa cgttacgtcg gtcaggccac cggctcacga tactcacaag aaggccggcc
     2701 acagtagagc acctcttcgg gtcgcaagtg acgacggtgg agtgggatgc gttgagtgca
     2761 gggacttggg agcgcagttt gcgcgacgct caggcggtga tcaatctcgc cggcgcaggt
     2821 atcgcggatg cccgttggac cgaccaccgt aagcgggtgc tgatcgagag ccgtctccgg
     2881 cccacgcggc tcctggtcga ggcctgcgca cgtctgagcg tgaagcccca ggtctttatc
     2941 agcgcgtctg gcgtcgggtt ctacggcccg cagggcactg aggtgctcga tgaatcgagc
     3001 gggcgaggcc gaggatttct ttccgatctc tgcgtccaat gggaatccgc tgcattagaa
     3061 gctgcttcgc agggaatacg agtcgtgtgt atgaggacag ggatggtcct cgagcaggac
     3121 gggggagccc tgccgcgtat cgcactcccc ttccggctgt ttcttggcgg acccgtgatg
     3181 cccggcactc aatggatctc gtggattcat cgagacgatt tggttggttt gatcgaatgg
     3241 gccttggcga tgcctgaagt gagcggtccg gtcaacggcg tcgctccctc gcctgtgaca
     3301 atggcagaat tctgccggct gcttggtctc gttctcagaa ggccttcgtg gcttcccgtt
     3361 cctgaatttg cgttgagaat ggcgctggga cagttgggat caataatgac aacggggcag
     3421 agggtcgtcc cggccgttgc ggaacgtcac gggtacagat tccgctttcg ggaagttcag
     3481 cgagcgttac aggccatctt tcccgcaggg gcataaccat gaaaaaaaga ttccctatct
     3541 cgacggtctg gcagccggta ttctaaagag gaaaaacatt tgaatcgtag gagggaacca
     3601 tgaaatcaac aacactatta ttgattctgg gcgtgctgtc aggactgttg ttctggggag
     3661 gatccgcgat cgccgagccg tctgcggagc cctctttgaa gcaaagcgaa ggcatgcagc
     3721 tttcca
//
LOCUS       DASTQY010000065         5525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_959662, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000065 DASTQY010000000
VERSION     DASTQY010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959662"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFR82_02065"
     CDS             <1..257
                     /locus_tag="VFR82_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500415.1"
                     /translation="QGTFVFVVKGDQTVGVRAITVGVTHGEESSIESGLVPEDIVVVD
                     GAEKLRDGTKVEIRAPRDSPPPATGLPPAVGDVPRKGSRS"
     gene            254..3370
                     /locus_tag="VFR82_02070"
     CDS             254..3370
                     /locus_tag="VFR82_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtB/MuxB family multidrug efflux RND
                     transporter permease subunit"
                     /protein_id="HEU4500416.1"
                     /translation="MNPSRLFILRPVATTLSMAAIFLAGFIAYRLLPVSALPQVDYPT
                     IQVATFYPGASPDVMASSVTAPLERYFGQMPGLTQMTSTSSSGSSVVTLQFSLDLDLD
                     VAEQGVQAGINAASTFLPRDLPNPPVYSKVNPADAPILTLALTSETLPLSEVEDFAET
                     RFAQKISQMSGVGRVTISGGQRPAVRIQANPTTLAAYGLTLEDLRVALAAANVNQAKG
                     AFDGPRRASIINATDQLMVSRDYRPLIVSYRNGAPVHLSDVADVIDDVENIKQAAWMN
                     DVPAVILNIQRQPGANVIEVADRIKILLPQLQSAMPSSVHVTVLTDRTTSIRASVRDV
                     QFELMLAVALVILVIFLFLRTLSATVIPAAAVPLSLVGTFGLMYLMGFSLNNLTLMAL
                     TISTGFVVDDAIVMIENIARYIEKGDSPFQAALKGSQQIAFTVLSLTISLIAVLIPLL
                     FMGDVVGRLFREFAITLSVTILISAVVSLTLTPMMCARLLRHRRQTEQSAFYRHTQRL
                     LDRVIANYGASLRWVLNHQQATLTVAVGTLILTVLLYVMVPKGFFPVQDTGVILGITE
                     APQTISFAAMRDRQRAVARAVLEDPAVESLSSFIGVDGINTTLNSGRIQINLKPLKER
                     KVSASDVMQRLQSRVTAVGGITLFLQPVQDLTVENRVSRTQYQYTLEDADPEELNRWA
                     PALVESLQALPELRDVSSDQQNHGLAAMVNIDRNTAFRLGITPQLITDALYDAFGQRQ
                     VSTMFTQLNQYRVILEVMPEFQKGPQAMQFIGVRSFGGGQVPLNVFTTVTETTAPLTV
                     NRQAQFPAVTLSFNLAPGTSLGEAVKAIERVTQKVGLPPSIRRSFQGTAQAFRASLAN
                     EPLLILAALMTVYIVLGILYESYIHPLTILSTLPSAGVGALLALMLFGIEFSVIALIG
                     IILLIGIVKKNAIMMIDFALDAERKDGKHPTEAIYEACLLRFRPIMMTTMAALLGALP
                     LAIGSGVGSELRHPLGIAIVGGLIVSQVLTLYTTPVVYLAFDRLARRVRAGTAQAPIS
                     EALGTGPT"
     gene            3367..>5525
                     /locus_tag="VFR82_02075"
     CDS             3367..>5525
                     /locus_tag="VFR82_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU4500417.1"
                     /translation="MNISAPFIRRPVATTLLTIGVTLVGIIAFRLLPVASLPQVEFPT
                     IGVQANLPGASPETMASSVAAPLERQFTRIAGVTEMTSSSTLGSTSITLQFELSRDID
                     GAARDVQAAINAARADLPANLPSNPGYWKNNPADAPILLLSLTSDIVDRGVMYDAASS
                     ILQQKLSQIEGVGRVYVGGGSLPAIRVDLNPTALNKYGIGFGDVRRMLSATNVRRPTG
                     QLTDGTRAWEIRINDQIHKADDYLPLVVAYRDGRAVRLSDIATVETSVEDLRNMGMAN
                     MTPAVLVMVYRQPGANMIETVDRIRAVLPQLEASIPGSIKLSLFSDRTEVTRASLHEV
                     ERTLAISVLLVILVVFAFLRDVRASLIPSVAVPVSLISTFGVMYLLGYSLDNLSLMAL
                     TIATGFVVDDAIVVLENISRYREQGMPMLQAALRGAREITFTVLSMTLSLVAVFLPIL
                     LMGGMMGRMFREFAVTLSVAILVSLVVSLTTTPMMCATLLKPHRPGPHGRWYRISEGI
                     FEGMRGKYAGTLAWVLRHPRIMLGLTLATMGLSGYLYTVVPKGFFPQQDTGLITGVSE
                     AAQDVSFKEMMRRQEAIGDIVGKDPAVATYAMSIGTGGSVAALNNGRIFITLKPRSER
                     DVSADQVIARLRPKLEAVEGARLFMQAAQDVNVGGRAARTQFQYTLQDSNFDELNEWA
                     PKVLEKLKALPELRDVASDQQMSGTALMLTIDRDQASR"
BASE COUNT         1056 a         1676 c         1636 g         1157 t
ORIGIN      
        1 cccagggcac gttcgtcttt gtggtgaagg gagaccagac agtcggcgtg cgggcgatta
       61 cggtcggtgt cactcatgga gaggaatcgt cgattgagtc gggtctggtt cccgaagaca
      121 tcgtggtcgt ggacggggca gaaaagcttc gagatggaac caaagtcgaa atccgggcgc
      181 cgcgcgactc gcctccgccc gctaccgggt tgccgccggc cgtaggggac gtgccgcgta
      241 aaggaagccg ctcgtgaatc catcccgtct ctttattctg cgtccggtag cgacaaccct
      301 gtcaatggcc gctatcttcc ttgccggatt catcgcgtac aggttgttgc cggtatctgc
      361 gctaccgcaa gtcgattatc cgaccattca ggtggccacg ttctatcctg gcgcgagtcc
      421 cgatgtgatg gcatcgtccg tcaccgcccc tcttgaacgc tatttcgggc agatgcccgg
      481 attgactcag atgacctcca caagttccag cggcagctcg gtggtgacgt tgcaattcag
      541 tctcgacctc gaccttgatg tcgccgaaca gggcgtacag gccggcatca acgcggcctc
      601 tacatttctg ccgcgggatc ttccgaaccc gccggtctac agcaaggtca acccggccga
      661 cgcgccgatt ctgaccctgg cgctgacgtc ggagacgctc cctctatcgg aggtggagga
      721 ttttgcggaa accaggtttg ctcagaaaat ttcgcaaatg tccggcgtgg ggcgggtgac
      781 catcagcggt ggacagcgac cggcggtgcg catccaagcc aacccgacga cgttggcggc
      841 ctacggactg accctcgaag acctgcgcgt ggccctggcg gccgccaacg ttaaccaagc
      901 gaaaggcgca ttcgacggcc cgcggcgggc ctcgatcatc aatgcgacgg atcaactcat
      961 ggtcagccgg gactaccggc cgctgatcgt gtcgtatcgc aacggggccc ctgtgcatct
     1021 gtccgacgtc gcggatgtca tcgatgatgt cgagaatatc aaacaggcag cctggatgaa
     1081 cgacgtcccg gcggtcatcc tcaacattca gcgtcaaccg ggcgcaaacg tcatcgaggt
     1141 ggcagaccgc attaaaattt tgctcccgca gcttcagagt gcaatgccat cgtccgtcca
     1201 tgttacggtg ctgaccgacc gtacgacatc aatcagggct tcggttcggg acgtgcagtt
     1261 cgaattgatg ttggcagtcg cgctcgtgat cctggtgatc tttctttttc tccgaaccct
     1321 atccgctacg gtcatcccag cggccgccgt tccgctttcc ctggtcggga cgttcgggct
     1381 catgtacctg atggggttca gcctgaacaa cctcacgctg atggccctca ccatttccac
     1441 gggttttgtc gtggacgatg cgatcgtgat gatcgaaaac atcgcacggt acatcgagaa
     1501 gggggattcg ccgttccagg ccgcactcaa gggttcccaa cagatcgcct tcaccgtgct
     1561 ctcgttgacc atctcgctca tcgccgttct cattccgctg ctgttcatgg gggacgtcgt
     1621 cgggcggttg ttccgagagt ttgccatcac cctgagcgtc acgatcctca tctcggccgt
     1681 ggtgtcgttg acgcttaccc cgatgatgtg cgccagactg ctacgacaca gacggcagac
     1741 cgagcaaagt gcgttttacc gacataccca gcggctgctc gatcgggtca ttgcgaacta
     1801 tggggcatcg ctacgatggg tactgaatca tcagcaggcc acgctgaccg tagcggtggg
     1861 cacgctgatt ctgaccgtcc tgctctacgt gatggtccca aagggttttt tccccgttca
     1921 ggataccggt gtgatcctgg gcatcaccga agcaccgcag acaatttcat tcgccgccat
     1981 gcgggatcgg cagcgggcag tggccagagc ggtactcgag gatccggccg tcgagagcct
     2041 ttcttcgttc atcggcgttg atggtatcaa taccacgctc aacagtgggc gaattcaaat
     2101 caatctcaaa cccctcaaag agagaaaggt gagcgcaagc gacgtcatgc aacgtctaca
     2161 gtcccgcgtt accgccgtag gagggatcac gttgtttctg cagccggtgc aggacctcac
     2221 ggtcgagaat cgcgtgagcc gtacacaata ccaatatacg ctcgaagatg cggacccgga
     2281 ggaactgaac cggtgggcgc ccgccttagt cgagtccttg caggcgcttc ccgaactgcg
     2341 cgacgtgagc agtgatcagc agaaccatgg cttagccgcc atggtgaata tcgatcggaa
     2401 taccgccttc cgccttggaa tcactcccca gctgattacc gatgccctct atgacgcgtt
     2461 cggtcagcgc caagtctcca ccatgtttac gcagctcaat cagtatcgag tcattctcga
     2521 agtgatgcct gagttccaaa aagggccgca ggccatgcaa ttcatcggcg tgcgctcgtt
     2581 cggcggcgga caggtcccgt taaacgtctt cacgaccgtg acagaaacta cggcgccgct
     2641 cactgttaat cgtcaagcgc aattccccgc ggtcacgctg tccttcaatc tcgcgccagg
     2701 aacgtcgctc ggtgaggcgg tcaaagccat cgagcgggtc acgcaaaagg tcggcctgcc
     2761 gcctagcatc cgccgcagtt tccaaggaac ggcccaggca ttccgtgctt cactggcgaa
     2821 tgaaccactg ctgattttgg cagcgctgat gacggtgtac atcgtgctcg gcatcctcta
     2881 tgaaagctac atccatccgc tcacgatcct ctccacactg ccgtcagcgg gcgtcggtgc
     2941 gctactggcg ctcatgctgt ttggcatcga attcagcgtg atcgccctca tcggcatcat
     3001 tttgttgatc ggcatcgtga aaaagaatgc catcatgatg atcgattttg cgctcgatgc
     3061 ggagcgtaag gacgggaaac accccaccga agcaatctat gaggcctgtc tcctccgttt
     3121 tcgccccatc atgatgacga cgatggctgc cttgctaggc gcccttcctc tggcgatcgg
     3181 ctcaggagtc ggttcggagc tgcggcatcc tttgggcatt gccatcgtgg gtggactcat
     3241 tgtaagccag gtgttgaccc tctacacgac gccggtcgtc tacctcgcat tcgatcgctt
     3301 ggctcgacgc gttcgcgccg gaacagctca ggcgccgatc tcggaggcgc tcggcaccgg
     3361 gccgacatga acatctccgc accatttatc cgacgtccgg tcgccacgac gttgctcacg
     3421 attggcgtca cgctcgtcgg aatcatcgcc ttccggttac tgccggttgc ttctcttccg
     3481 caagtggaat tccccacgat cggtgttcag gcgaatctcc ccggcgcgag tccggaaacg
     3541 atggcgagtt cggtcgcggc tccgctggaa cgtcagttca cccggatagc cggcgtgacg
     3601 gagatgacct catcgagcac gctcggctcg accagcatca cattgcagtt cgagctgagc
     3661 cgcgacatcg acggagctgc ccgcgatgtc caggccgcga tcaacgccgc ccgtgccgat
     3721 ctgccagcca accttccgtc gaatcctggt tactggaaaa acaatccggc cgatgcgccc
     3781 attctccttc tctcgctgac ctcggacatc gtggaccgcg gtgtaatgta cgatgctgct
     3841 tcgagcatcc tgcagcaaaa gctctcgcag atcgagggtg ttggcagagt gtacgtggga
     3901 ggaggctcgc ttccggccat tcgcgtggat ctcaatccta cggcgctgaa caaatacggc
     3961 atcggattcg gcgacgtccg tcgcatgctg agcgccacaa acgtgaggcg gccgaccggg
     4021 caactgactg acgggacgcg agcctgggaa attcggatta atgatcagat ccacaaggcg
     4081 gacgactact tacctctcgt cgtcgcctat cgggatggac gggcggtgcg actgtcggac
     4141 attgcgacag tggagacgtc ggttgaagac ttgcggaata tgggcatggc caacatgacg
     4201 cccgctgtgc ttgtgatggt ctatcggcag ccaggtgcga acatgatcga gacggttgat
     4261 cgcatacgcg ctgttttgcc tcaactggag gcctcaattc caggcagcat caagctgtcc
     4321 cttttcagcg accggacaga agtcacccgg gcgtcgctgc acgaggtcga gcggacactg
     4381 gctatctcgg tcctgctcgt gattctggtc gtgtttgcct ttcttagaga cgtacgtgcc
     4441 agtcttattc cctctgtcgc ggtgccggtc tcgctcatca gcacgttcgg cgtgatgtac
     4501 cttctcggct atagtctcga caacctctca ctcatggcgc tcacgatcgc aaccggattc
     4561 gtcgtcgacg acgcgattgt cgtgctggaa aacatcagcc ggtaccgaga acagggcatg
     4621 cccatgctgc aggccgcgtt gcgcggtgcg cgagagatca cgttcacggt cctgtcgatg
     4681 acactctcgc tcgtcgcggt gtttcttccc attttgctca tgggaggcat gatgggccgg
     4741 atgttccgcg agttcgccgt cacgctttcg gtggcgattc tcgtctccct cgtggtgtcg
     4801 ctgaccacaa cgcccatgat gtgcgcgaca ttattgaagc cgcaccgccc cggaccgcac
     4861 ggaaggtggt atcgaatcag cgaagggatc tttgagggca tgcgtgggaa atatgccggg
     4921 accctcgcgt gggtgctgcg acatccccgc atcatgctcg ggctcacatt ggcaaccatg
     4981 gggctcagcg gctaccttta tacagtggtg ccgaaaggct ttttcccgca gcaggacaca
     5041 ggcctcatta ccggtgtctc ggaagcggcg caggatgttt cgttcaagga gatgatgcgg
     5101 cggcaggagg cgatcggcga tatcgtcggc aaggatccgg cggttgccac ctatgccatg
     5161 agcatcggca cgggcggcag cgtggcggcg ttgaacaacg gccgcatctt catcacgctg
     5221 aagccgcgca gcgaacgcga cgtgtcggcc gatcaggtga tcgcgcggct ccggccgaag
     5281 ctggaggcgg tggaaggcgc gcgcctgttc atgcaggccg cgcaagatgt gaatgtcggc
     5341 gggcgcgcgg cgcgcacgca gttccagtac acgctgcagg actccaactt cgacgagctc
     5401 aacgagtggg cgccgaaggt gctggagaag ctgaaagccc ttccggagct gcgggacgtg
     5461 gcctccgatc agcagatgtc cgggacggcg ctgatgctga ccatcgaccg cgaccaggcc
     5521 tcgcg
//
LOCUS       DASTQY010000066         4913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1363607, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000066 DASTQY010000000
VERSION     DASTQY010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363607"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VFR82_02080"
     CDS             <1..875
                     /locus_tag="VFR82_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4500418.1"
                     /translation="QSINYEGATPLRVNADRTYYNWAPEMSLTWKPAEGYRHWIRAST
                     GYGIPQFNNLLRDPVTGQPGTNFDLKPQKNLNTEIGTESRLTKDLVVELVGFWTFFKN
                     EIITQTISGNNVASVNADSSQYRGIEARYDWRPLTGLRFSGAYTHLDARYVNFSDRTA
                     AGFLVRDGNKVPNVPTDIFNTKVEYDHAPTGWGGWVEGSYYNSYFLNNSNTFGIPSYI
                     IMNVNVHKSIEIKNSWIRFAKFYLELDNIADKKYAASGQVIGGETTAAAAGQQAFFAG
                     YGRAIYGGVTLGLF"
     gene            983..2044
                     /locus_tag="VFR82_02085"
     CDS             983..2044
                     /locus_tag="VFR82_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4500419.1"
                     /translation="MTVRAARLFWVILSVVSCLFFPDGGSAATPADSDRRAVEQATEL
                     FRGGDADAAIGALRKATESGSSSAEAYHLLGQIYFKGKKKPQDAVEAYTHALKLKPAY
                     PDALNDLAEVYVAQGRTAEAEQTLKRAIEVAPKREESYVNLARLYESRHDVPAAINTY
                     QRLLTVRPTSADGLFGLAILYEGQGNNKSARELLARLTKANPAHADGWYQAGRLAERS
                     NDLLEAAYAYRQAIAAKPNLVDAHYNLGFILRSQNKPAEAEKEFLEVIRYRPEYAEAH
                     MNLGVVYTSMNKLEEAEQEYERAVALKPDYAEAHYNLGVFYELHRKDMPRALAQYHKY
                     RNLGGRDDRVERIIGTAGR"
     gene            complement(2221..2727)
                     /locus_tag="VFR82_02090"
     CDS             complement(2221..2727)
                     /locus_tag="VFR82_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HEU4500420.1"
                     /translation="MSLCLVVLGCASNPETGEGSPANQISFLQVKAAPDSYRGQSVLF
                     GGEVLSAKRLKDGTRIEILQLPLDRSGEPGTDRMKSEGRFIAMQRDFLDPATIPPGTR
                     VTVTGEVTGSTTLPLDETEYTFPVIETRHIEVLPPPESSLPTRPYMGSHYYGPYGSPY
                     WRPWPYRW"
     gene            2844..3494
                     /locus_tag="VFR82_02095"
     CDS             2844..3494
                     /locus_tag="VFR82_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU4500421.1"
                     /translation="MRRYPGMWAMMVGMLIATVQLPTTDTVQASGPPEFKGKYEILQD
                     ERSTHQPGKVKLIEFADFYCPHCHYFDGEGLPILEKEFGKKLETTMVGFPVIRGKLPT
                     PFDMYEQAKLMGKGNEMKKVLFRTIHKDKVTGVLDRSIREVLIKEVGLDPKVFEEGLA
                     SGKPAQLFEDGKKWGERIKLQQTPTILLDGNIKVEKIDPDNLKLLIQSILDGDAKK"
     gene            3597..>4913
                     /locus_tag="VFR82_02100"
     CDS             3597..>4913
                     /locus_tag="VFR82_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEU4500422.1"
                     /translation="MAVDPVCGMTVDPATAAGQYDYQGVTYYFCATSCLETFKRDPQR
                     ALQARQPGLITLGKNNPLPMMPASPSVESGNIDPVCGMTVQPESAAGSYVHEAKRYYF
                     CSPGCLARFRENPTHYLIPPEQRLQKPIAVPAGATVEYICPMDPEIVETKPGVCRICG
                     MALEPKVVTLDERPNPELLDMSRRFWISVGPTLLVMLFAMADMIPMLRGKIDYSARNW
                     IQLLLATPVVLWGGWPFFERAWKSLVLRAPNMFTLIGLGTGAAYLYSAVATVAPRMLP
                     ASFRAHDGSFAVYFEAAAMITVLVLLGQVLELRARSQTTSAMKALLRLAPKTARRIDG
                     GRETDVSLDQVRVEDLLRVRPGERVPVDGVVIEGTTAIDESMVTGEPLPVGKAPGASV
                     TGGTINGAGSILIRAERVGSETLLARIVQMVSEAQRSRAPIQRVADI"
BASE COUNT         1136 a         1298 c         1449 g         1030 t
ORIGIN      
        1 tccagtccat caactatgag ggagccacgc cgttgcgagt caacgcggat cgcacgtact
       61 acaactgggc gcccgagatg tccttgacct ggaagccggc tgagggttat cggcattgga
      121 tccgggcttc cacagggtac ggcattccac aattcaataa tttattgaga gatcccgtca
      181 cgggccaacc tggaaccaat tttgacctca agccgcaaaa aaatctcaat acggaaatcg
      241 gcacagagtc gagactgacg aaggatttgg tcgtggaact ggttgggttt tggaccttct
      301 tcaagaatga aatcattact cagaccattt cagggaacaa cgttgcgtcg gtgaatgccg
      361 actcctcgca atatcgcggg atcgaggctc gatatgactg gcgccccctt acgggattgc
      421 gtttctcagg ggcctacacg catctcgatg cgagatatgt caatttctcg gatcgcaccg
      481 cggcaggctt cttggttcgc gatgggaaca aggtgcccaa cgtgccgact gatattttca
      541 acaccaaagt tgagtacgat cacgcgccta ccggttgggg agggtgggta gaaggcagct
      601 attacaacag ctatttcttg aacaacagca atacgtttgg catcccgtcg tatatcatca
      661 tgaacgtgaa cgtccacaag agcatcgaaa tcaaaaactc ctggatacga ttcgcgaagt
      721 tctatctgga gctggataac attgcagaca agaagtatgc ggcgtccggc caggttatag
      781 gcggggaaac gactgctgca gcagccggtc agcaggcctt ctttgccgga tacggccgag
      841 ccatctatgg aggtgtgacg ttgggccttt tctagccttg agtttctgcg aaggggccac
      901 taacacacgt cccctcacgt ttgatttcga taccggcccc ccgtatagtt ggaacacacc
      961 tcgcgtagca cggagcgctc tcttgacggt acgtgcagct cgactctttt gggtgattct
     1021 aagcgttgtt tcttgccttt tctttcctga cggtgggtct gctgccacac ctgccgattc
     1081 ggacaggcgc gccgtcgagc aggcgaccga actcttcagg ggcggcgatg ccgatgccgc
     1141 tattggcgcc ttgcgcaagg cgacagagtc cggttcttct tcagcagaag cctaccatct
     1201 attgggccag atctatttca aaggcaaaaa aaagcctcag gacgcggtcg aggcctatac
     1261 gcatgcgctc aagctgaaac cggcgtatcc cgatgccctc aatgatctgg cggaagtgta
     1321 tgtcgcacag ggcaggacgg cggaggctga gcagacgctt aaacgggcga tcgaagtggc
     1381 gccaaagcgt gaagagtcct atgtgaacct tgcacggctg tatgagagcc ggcacgatgt
     1441 tcccgccgcg atcaacacct atcagcgctt gttgactgtc cgtcccacga gtgcggacgg
     1501 actgtttggg ctggcgattc tgtacgaagg gcaaggcaac aacaagtccg cacgcgaatt
     1561 gctcgcacgt ttgaccaaag ccaatcctgc tcatgccgac ggatggtatc aagccggccg
     1621 gttggccgaa cggagcaatg acttgctcga ggccgcctat gcgtaccggc aggccattgc
     1681 cgcaaaaccg aacctggtcg atgcccacta taaccttggg ttcattctca ggagtcaaaa
     1741 caagccggcg gaagccgaga aagaattcct agaggttatc cgttatcgtc cagagtatgc
     1801 cgaggcgcat atgaaccttg gggttgtcta tacgagtatg aataagctgg aagaggcaga
     1861 acaggaatat gagagagccg tggccttgaa accggactac gccgaggccc actacaacct
     1921 tggcgtgttc tatgagttac atcgaaaaga catgccgcgg gcgctcgcgc aatatcacaa
     1981 gtaccgaaac ttgggcggtc gagacgaccg ggtggaacgg atcattggca ccgccgggcg
     2041 ttaacgcgct cagggtgttc aataaggcag ttgttctcac ccgcccagcc ccggcacgcc
     2101 gagacgcgcc tctccgctgg caaggccgca gcgaatgaag aggtgaagcg tacatattct
     2161 gatacgttga acctctgaat gacgcgagaa ggaagctagc ggcctttttc aacaccctgc
     2221 ttaccagcga tacggccatg gccgccaata cggactacca tagggaccat aatagtgaga
     2281 acccatgtag ggtcgtgtgg gaagcgagga ctccggggga ggtaagacct cgatgtgacg
     2341 tgtctcaatc actgggaagg tgtattccgt ttcgtcaagg ggaagcgtcg tggagcccgt
     2401 cacttcacct gtcacggtca ctcgcgtgcc gggaggaatg gtcgcaggat ctagaaagtc
     2461 tctttgcatg gcaatgaagc gcccttcgga tttcatccgg tcggttcccg gctctccgga
     2521 gcgatccagc ggaagctgca aaatctctat tcgagtgccg tccttcaatc gttttgcgct
     2581 caagacttct cccccgaata ggaccgattg ccctctgtag gaatccggag cggctttgac
     2641 ttgaaggaac gaaatttgat tggcgggcga tccttccccc gtttctgggt ttgacgcaca
     2701 ccccaacacc accagacaga gactcatcgc tgagacagca gcccatcggg aaggcatgag
     2761 gcgaattata ccaaacagca aagggcagtc ggtattcgat ataatggccg gcggccgtat
     2821 gagcgtttga gaaaggagcg ggcatgagaa gatatccggg tatgtgggcg atgatggtgg
     2881 ggatgctgat cgccacagtt cagcttccga cgacggacac cgttcaggcg tcaggaccgc
     2941 ccgagttcaa gggtaaatat gaaattctgc aagacgagcg gtctacccat caaccgggaa
     3001 aggtcaagtt gatcgagttc gcggattttt actgcccgca ctgtcattac ttcgatgggg
     3061 aaggactgcc gattctagaa aaggaatttg ggaagaagct cgagacgacg atggtagggt
     3121 ttcccgtgat cagaggcaag cttccgacgc cctttgatat gtatgagcag gccaagttga
     3181 tgggcaaggg aaatgaaatg aaaaaggtcc tgttccgtac gattcacaag gacaaggtga
     3241 cgggcgtgct cgaccgctcg atacgcgaag tactgatcaa agaagtggga ctcgatccta
     3301 aagtgttcga ggaagggctt gctagcggca aaccggctca gttgtttgaa gacgggaaaa
     3361 agtggggcga gcggatcaag ttgcaacaga cacccaccat cctgttggac ggcaatatca
     3421 aggtcgaaaa gatcgatcca gacaatctca agctcctgat tcaaagtatt ctcgatgggg
     3481 atgccaagaa gtagccggtt cacggcttga ccgccggacc gagaagcacg cgtgacgcct
     3541 gtgctgtgcg agactcgggg ttcccgttcg agagaaactg ttccaacgat ctccatatgg
     3601 ctgtagatcc tgtttgcggc atgactgtcg atccggccac cgcagccgga cagtatgact
     3661 atcagggtgt gacctactat ttctgcgcga cttcctgcct tgagaccttt aagcgcgatc
     3721 ctcaacgggc actgcaggct cgtcagccag ggttgattac gttggggaag aacaacccac
     3781 tgccgatgat gccggcatct ccatcggtgg aatcggggaa catcgacccg gtttgcggaa
     3841 tgaccgtaca acctgaaagc gccgccggct cctatgtcca tgaagccaag cgctactatt
     3901 tctgttcacc cggctgctta gccaggtttc gtgagaaccc aacccattac ctgatccctc
     3961 cagagcagcg gctacagaaa ccaattgcgg taccggccgg tgcgaccgtg gaatacatct
     4021 gtccgatgga tcccgagatc gtcgagacca agccgggagt ctgccgcata tgcgggatgg
     4081 cgttggaacc gaaggttgtc accttggacg agcgcccaaa tcccgaactt ctggacatga
     4141 gcagacggtt ttggatcagc gtggggccga cgctgctcgt gatgctgttc gcgatggctg
     4201 acatgattcc aatgctccgc ggaaagatag actattcagc gagaaactgg attcagttgc
     4261 tgctggctac tccggtcgtc ctgtggggag gctggccttt ttttgaacgg gcatggaagt
     4321 cacttgttct ccgggctccc aacatgttca cgctgatcgg actgggaacc ggcgccgcct
     4381 atctgtacag cgcggtcgcc acggttgcac cgcgcatgtt gccggcgtcc ttccgcgcac
     4441 atgatggatc atttgccgtg tattttgaag ctgcagcgat gatcacggtg ctcgtgttgt
     4501 tagggcaagt cttggaattg cgcgcgcgca gccaaactac ctcggcgatg aaggccttgc
     4561 tgcgtttggc cccgaaaacc gccaggagga tcgacggcgg gcgtgagacc gatgtgtcgc
     4621 tcgaccaagt cagggtagaa gatctattgc gcgtgcgacc aggtgagcgg gtgccggtgg
     4681 acggagtggt catcgaaggc acgacggcga tcgatgagtc gatggtaacg ggagaacccc
     4741 tcccggtggg aaaggcacct ggcgcgtcag tgaccggggg gaccatcaat ggagcaggca
     4801 gcattctgat ccgggctgag cgtgtcggca gcgaaacctt gctggcgcgc atcgttcaga
     4861 tggtgagtga agctcaacgc agccgcgcgc ctatccagcg agtggccgac atc
//
LOCUS       DASTQY010000067         7840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1564221, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000067 DASTQY010000000
VERSION     DASTQY010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564221"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /gene="ileS"
                     /locus_tag="VFR82_02105"
     CDS             complement(<1..1696)
                     /gene="ileS"
                     /locus_tag="VFR82_02105"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU4500423.1"
                     /translation="MDYKSTLNLPKTDFPMKANLPQREPELLAWWEQERLYARIQEAG
                     KGRPRYLLHDGPPYANGRIHIGHALNKILKDIIVKSKTMAGFQAPYVPGWDCHGLPIE
                     HQVLKDLGEKKHALDAMGIRKLCREYAEKFYKIQREEFQRLGVLGDWQHPYLTMNPAY
                     EATIVREFGKFVERGGVYKGLKPVLWCTQDQTALAEAEVEYEDHVSPSIYVKFPIAGG
                     SAAVAAKLPGINLPSDVTTVSIVIWTTTPWTLPANQAVCLHQDFDYAFVRVGSEVVIV
                     ADKLVDSVAKTCNLEGYRVLAVRKGGEGFEGLETQRPLTTGLSPVLLGDFVTLEQGTG
                     CVHIAPGHGMEDYILVLEHNATASPGERLEILAPVDDAGRFTQAVPEFAGQHVFKANP
                     GIVDLLRTKGRLFGHGSLAHSYPHCWRCKSPVIFRATEQWFVSMDINALRQEALAEIN
                     RVKWIPPWGKDRINGMIANRPDWCLSRQRVWGVPIPAFTCAGCRRVLADPRIIDHIGR
                     IMDEHGADVWFNRSAAELLPAGTICPHCNGNQFEKERDILDVWFESGVSFAAVLKPC"
     gene            complement(1939..3042)
                     /gene="nadA"
                     /locus_tag="VFR82_02110"
     CDS             complement(1939..3042)
                     /gene="nadA"
                     /locus_tag="VFR82_02110"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEU4500424.1"
                     /translation="MNSVASLPRVVTDYQLLSAEELRQRIAEAKRTLGDQVMVLGHNY
                     QRDDVIEHADFRGDSLLLSKLAAERSERPHVVFCGVHFMAETADILSRTNQTVILPDM
                     AAGCSMADMAAIEQVDQCWETLGRFLPVEETVMPAVYVNSAAVLKAFCGEHGGITCTS
                     SNARAVIEWCWARREKILFFPDEHLGRNTANKMGIARQHMIVWDPFQPNGGNTRDAIQ
                     RAKLILWKGHCSVHQMFQAAHVDNFRKQYPQGQVIVHPECHEDVVNKADLAGSTEFII
                     KTVTAAPAGTVWAVGTELNLVNRLKREMTDKKVFFLSSTVCQCATMFRIDAAHLCWAM
                     ENLVDGHIVNHIVVPEDDKRWARIALDRMMSVS"
     regulatory      3206..3308
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3394..5277
                     /gene="thiC"
                     /locus_tag="VFR82_02115"
     CDS             3394..5277
                     /gene="thiC"
                     /locus_tag="VFR82_02115"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEU4500425.1"
                     /translation="MTTNGHPTGTLPITAQPFPASRKVFVAGTQPGVRVPMREIALTP
                     TKSPNGGAPTQNEPLVVYDTSGPYTDQSVTIDLRMGLAPLRQDWILGRGDVEQLSDVT
                     SVYGRLRAADPKLTDLRFQHIRRPLRAKRGLNVTQLHYARRGMITPEMEFIAIRENQS
                     REVACELASRNGHGGGVAQHPGQAWGASIPKAITPEFVRDEVAKGRAIIPANINHPET
                     EPMIIGRNFLVKINSNIGNSAVSSTIEEEVEKMIWSTRWGADTVMDLSTGKNIHETRE
                     WIIRNSPVPIGTVPIYQALEKVGGKAEDLTWDIFRDTLIEQAEQGVDYFTIHAGVRLA
                     YVPLTANRMTGIVSRGGSIHAKWCLAHHQENFAYTHFEEICDIMKAYDVSFSLGDGLR
                     PGSIADANDESQFAELETLGELTKIAWSHDVQVMIEGPGHVPMHLIQVNMEKQLKECH
                     EAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWYGCAMLCYVTPKEHLGLPDREDVKTG
                     VITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLSLDPDTAKEFHDETLP
                     DQAAKVSHFCSMCGPHFCSMKITQDVREYAASKRVTEQQAIEIGMKEKAEEFKQSGSE
                     IYR"
     gene            5330..6130
                     /locus_tag="VFR82_02120"
     CDS             5330..6130
                     /locus_tag="VFR82_02120"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HEU4500426.1"
                     /translation="MGKPKPAPLRERDITRQIAREYYKEFDQLIESDVIIVGAGPSGL
                     ICAHDLADMGFRTVIIEQSLALGGGFWHGGYLMNKATICAPAHKILEEIGVPCKKVKD
                     CEGMYIVDPPHATASLIAAAYGAGARVLNLTKVVDLILRREGVLEGVVVNNTTAEMAG
                     HDIIHVDPIALESKLVVDATGHDAVIVNLLHKRNLYTEVPGNGAMWVARSEEDVMDHT
                     GEVYPNCFVIGLAVAAVYGTPRMGPAFGSMLLSGRYGAELIKKKLKQE"
     gene            6247..6792
                     /locus_tag="VFR82_02125"
     CDS             6247..6792
                     /locus_tag="VFR82_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4500427.1"
                     /translation="MLVAGFKSRIMDVQDFLVQGEGSEEDKREAWELFQQAYQRQMKG
                     ELEEAVSLYRQSIATHPTAEAHTFLGWTYSFMGRLDDAIEECHRAIGRDPEFGNPYND
                     IGAYLIEKGELDDAIPWFQKAMHAKRYESPAFPHLNLGRVYEKKGNWTEAISSYKQAL
                     ALNPDYVLAKKSLGRLVSMLN"
     gene            6827..7216
                     /locus_tag="VFR82_02130"
     CDS             6827..7216
                     /locus_tag="VFR82_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU4500428.1"
                     /translation="MTHIHSKTILVAVTDIFFYTKVRDALLPNGYTLERARSHEDIAA
                     RASTTPPVAVILNMNDETLNAFQVLETLKADPRFHALPILAFANHEEVDTWNRAKSMG
                     VTKIVSRNEFSMRTRDLVEGVISHIPS"
     gene            7233..>7840
                     /locus_tag="VFR82_02135"
     CDS             7233..>7840
                     /locus_tag="VFR82_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HEU4500429.1"
                     /translation="MTTNTVMKHSRLHDHLAKLGATFEAVTGWLMPVHYGQWVDEYRA
                     VREGVGLADLSHRGKLRVTGDDRVKWLQSVISNDILPLQPGQGRYSSLLTHKGKMLTY
                     FRCYMQTDAVILEDAGEIGDQTYAALRKFLLYGTKAKLENCRESWGLLLVSGPKAATV
                     VKAAFGTDLADLKPVNFIPAAIGGQTALIIRSEETGEQDVEIL"
BASE COUNT         1807 a         2244 c         2220 g         1569 t
ORIGIN      
        1 ggcagggctt caacacggcc gcgaagctca cccctgattc gaaccagacg tctaggatgt
       61 cgcgttcctt ctcgaactgg ttcccattac agtgtgggca tatcgttccc gccggcaaca
      121 attctgctgc agaccgattg aaccacacgt cggcaccgtg ctcatccatg atccgcccga
      181 tatggtcgat gatcctcggg tcggcgagca cccggcggca gccggcacag gtaaatgcgg
      241 gaatcggcac gccccaaacg cgctggcgag agaggcacca atcgggtcga ttcgcaatca
      301 tcccattgat ccggtccttg ccccagggtg gtatccattt cacacggttg atctctgcca
      361 aggcctcctg ccgaagagca ttgatgtcca tcgagacaaa ccactgttcc gtggcgcgaa
      421 agatcacagg acttttacag cgccagcaat gaggatagga atgagccagc gagccatgac
      481 cgaagagccg gcctttcgtt cggagaaggt ccacaatccc cggattcgcc ttgaagacat
      541 gttgcccggc aaactccgga accgcctgag taaatcggcc ggcgtcgtca acgggagcga
      601 gaatttcgag ccgttcaccc ggcgacgccg tcgcgttgtg ctcgagcaca aggatgtagt
      661 cttccatgcc atggcccgga gcaatatgca cacaaccagt cccctgctcc agcgtcacga
      721 aatctcccaa gagcacaggt gagaggccgg tggtcagcgg tcgttgggtt tcaagccctt
      781 cgaatccctc cccccctttt ctcaccgcga gcacacgata cccctcaagg ttgcaggtct
      841 tcgccacgct gtcgaccagc ttatcggcga cgataacaac ttctgatccc actcgaacaa
      901 aagcatagtc gaagtcttga tgtagacaga ccgcttgatt ggccggcaag gtccacggag
      961 tggtggtcca gatgactatt gagacagtgg tgacatcaga aggcagattg atgccgggca
     1021 gcttcgctgc gactgcggcg gatccccctg ctatgggaaa cttgacgtaa atcgacggcg
     1081 aaacatgatc ttcgtactca acttccgcct ccgccaacgc ggtttgatcc tgggtgcacc
     1141 agaggacggg cttcaaacct ttatagacac cgccccgctc cacaaatttg ccaaactccc
     1201 ggacgatcgt cgcctcgtac gcaggattca tcgtcaggta cggatgctgc caatcgccga
     1261 gaacgcccaa ccgttgaaat tcttctcgct ggatcttgta aaatttctcg gcgtattcgc
     1321 ggcaaagttt gcgaatcccc atcgcgtcca aggcgtgctt cttctcgccc agatctttga
     1381 gcacctggtg ctcgatcggg aggccatggc agtcccatcc cggaacatag ggggcctgga
     1441 agcctgccat cgttttggat ttgacgatga tgtccttcaa aatcttgttc agcgcatggc
     1501 cgatatggat tcggccgttc gcgtagggcg gtccatcgtg caggagatag cggggccggc
     1561 ctttgccggc ttcttgaata cgcgcataca ggcgttcttg ctcccaccac gcgagcaact
     1621 ccggctcgcg ctggggcaga ttggccttca tcggaaaatc ggttttcggc agattgagag
     1681 tcgacttgta gtccatgggt atagacagtt tgggcggaaa tgaagtgaat ataccggaac
     1741 gtcgagcgag gtactagccc ttagccttcg tgcgagatcg tcaaaacgac ctacagcaag
     1801 acggcagcga gagagagcct gaagcgtacg gttccatgta cgatgaatgc ttgagcgacg
     1861 cgagaacgaa gttttggtga agggcgcgtc tcggcgagcc ggagttgcgg gtgagaagag
     1921 gatattttca cgataccttt agctgacgga catcatgcga tcgagggcaa tcctggccca
     1981 acgcttgtca tcctccggca cgacaatatg attcacgatg tgcccatcga cgagattttc
     2041 cattgcccag cagaggtgtg cggcatcaat ccgaaacatc gtcgcacact gacacacggt
     2101 ggaggacaga aagaacactt tcttatcggt catctcacgt ttcaaacgat tgactaagtt
     2161 cagctcggtt ccgaccgccc agaccgtccc ggccggcgca gcggtgacgg tcttgataat
     2221 gaactccgtc gagccggcga gatccgcctt gttgacgaca tcttcatgac actctggatg
     2281 tacaatcacc tgaccctggg gatactgttt ccgaaaattg tccacgtggg ctgcctgaaa
     2341 catctggtgc acgctgcaat gacccttcca cagaatgagt ttcgcccgtt ggatagcgtc
     2401 ccttgtattg ccgccattcg gttgaaacgg atcccagacg atcatgtgct gtcgagcaat
     2461 gcccatcttg ttggccgtat tgcgtcccaa gtgttcgtca gggaaaaaca gaatcttttc
     2521 acgacgcgcc cagcaccatt cgatcaccgc cctggcattg gaggaggtgc aggtgatgcc
     2581 gccgtgctcg ccgcagaacg ccttgaggac tgctgccgaa ttgacataca ccgccggcat
     2641 gacggtctcc tcgacaggta agaaacgacc cagcgtctcc caacactgat ccacctgttc
     2701 aatcgcggcc atgtcggcca tggaacaacc ggcggccatg tcggggagaa tgaccgtttg
     2761 attagtccgg ctcaggatgt cggcggtttc cgccataaag tgaacgccgc aaaagaccac
     2821 atgcggtcgc tccgaccgtt cagccgccaa cttggaaagg agcagcgaat ctccacggaa
     2881 atccgcatgt tcgatgacgt cgtcccgctg atagttatgg ccgagcacca tcacctggtc
     2941 tccgagcgtc cgcttcgcct cagcgatgcg ctggcgcaat tcctctgcgg acaggagctg
     3001 gtaatcggtc accactctgg gtaagctcgc aacgctgttc acaaggtcct caggaatatc
     3061 atttagatcg tcattctacg ataagaatta ccggccaatc aatgaagcag ggtcgggaag
     3121 aagaccttat cagggaccat tcgggagcag cgggataggg ctattaaccc gttgacttca
     3181 tacggaggcc ggcgatacga tttcatgcca gagctggggg ggccacgcgg ctgagaatcc
     3241 gagcgatcgg atgacccttc gaacctgacc tgggtaatac cagcgtaggg aagcggatca
     3301 ctgacgaccg tctcagccgc actctcctcc tgcaaggtgc ggctttttta ttgccccacc
     3361 tcgatcgttc acgatccgat gaaaggatct ttcatgacga ccaatggcca tccgaccggc
     3421 actcttccca tcaccgccca gccgtttccg gcctcgcgaa aagtattcgt cgccggcacc
     3481 caaccagggg tgcgtgtgcc gatgcgagag atcgccttaa cgcccacaaa atcaccgaac
     3541 ggcggggcac ctactcagaa cgagccgctc gtcgtctatg atacctccgg accctacacc
     3601 gaccaatcgg tgacgatcga tctgcgcatg gggcttgccc cccttcgaca agattggatt
     3661 ctcggtcgcg gcgacgtcga gcagctgtct gacgtgacat cggtatatgg ccgcctgcgt
     3721 gccgccgacc ccaagctgac cgatctccga ttccagcata tccgtagacc gctgcgtgcc
     3781 aaacgaggac taaacgtcac ccagctgcat tacgcccgcc gtgggatgat cacgccggag
     3841 atggaattca tcgcgatccg tgaaaaccag tcgcgcgagg tggcctgtga gctggcgtcg
     3901 cgcaacggtc atggcggagg cgtcgcccag catcctggcc aggcgtgggg tgcctctatc
     3961 ccgaaggcga tcacgccaga atttgtgaga gatgaggtcg cgaaggggcg tgcgatcatt
     4021 cccgccaata tcaatcatcc ggaaacagag ccgatgatca tcgggcgcaa tttcttggtg
     4081 aagattaatt cgaatatcgg aaattccgcc gtcagctcga ccattgagga agaagtcgaa
     4141 aagatgatct ggtcgacgcg gtggggcgcc gataccgtga tggacctctc gaccgggaaa
     4201 aacatccatg aaaccagaga gtggatcatc cgcaattcgc ccgtgccgat cgggacggtt
     4261 cccatctatc aagcgcttga gaaagtcggc ggcaaggcgg aagacctcac atgggacatt
     4321 tttcgtgaca cgttgatcga gcaggccgag cagggggtcg attacttcac catccatgcg
     4381 ggcgtccggc tcgcctacgt gcccctgacc gccaatcgta tgaccggcat cgtgtcccga
     4441 ggcggctcga tccatgccaa gtggtgtctg gctcatcacc aagaaaactt cgcctacacg
     4501 catttcgaag aaatctgcga catcatgaag gcctacgacg tgtcgttcag cctcggggac
     4561 ggcctacggc cgggttcaat cgcagacgcg aacgatgaga gccagttcgc cgaactcgaa
     4621 acgttgggcg aactgacgaa gatcgcctgg tcgcacgacg tgcaggtcat gatcgaaggg
     4681 cccggccacg tccccatgca tctgatccag gtcaatatgg agaagcagct taaggaatgc
     4741 cacgaagcgc cgttctatac cttggggccg ctcaccaccg acatcgcccc ggggtacgac
     4801 catattacgt ccgggatcgg cgccgcgatg atcggctggt atggctgcgc gatgctctgt
     4861 tatgtgacac ccaaagaaca tctgggattg ccggacaggg aagatgtgaa gacaggcgtg
     4921 atcacctaca agatcgccgc gcatgcggcc gacctggcaa aagggcatcc gggagcccag
     4981 atccgggaca acgccctctc caaggcccgc ttcgagttcc gttgggaaga tcaattcaat
     5041 ttgtcgctcg atcccgacac ggcgaaggag ttccatgatg agacgctgcc cgaccaggca
     5101 gctaaggtct cgcatttttg cagcatgtgc gggccacact tctgctccat gaagatcacg
     5161 caagacgtcc gcgaatatgc ggccagcaaa cgggtgaccg agcagcaagc gatcgagatc
     5221 ggcatgaagg aaaaagcaga ggagttcaaa caatccggtt cagaaattta ccgttagcac
     5281 tgcatcgggt ggctgcccac ctataccggc atccaccgag gagcacccca tgggaaaacc
     5341 caagccagcg cccctgagag agcgcgacat tactaggcag attgcccgcg agtactacaa
     5401 agaattcgat cagttgatcg aaagcgatgt catcatcgtc ggtgccggtc cttcgggtct
     5461 catttgcgcg catgatctgg cagacatggg cttccgcacc gtcatcatcg agcagagcct
     5521 cgcgctgggc ggcgggttct ggcacggcgg ctatctgatg aacaaggcca cgatctgtgc
     5581 tccggcccat aagatcctcg aggaaatcgg tgtgccatgc aagaaggtaa aagactgcga
     5641 aggcatgtat atcgtcgatc cgccgcatgc caccgcgagc ctgattgctg cggcttacgg
     5701 ggccggcgcg agggtgctca atctcaccaa ggtcgtcgat ttgatccttc gccgggaagg
     5761 tgtactcgaa ggcgtcgtgg tcaataacac gaccgccgag atggccggcc acgatatcat
     5821 ccatgtcgat cccattgcgc tcgagagcaa acttgtggtc gatgcgaccg gtcatgacgc
     5881 agtcatcgtg aacctcttgc acaagcgaaa cctgtataca gaagtacccg gcaacggcgc
     5941 gatgtgggtc gcgcgttcag aagaggatgt gatggaccac accggagaag tgtacccaaa
     6001 ctgtttcgtc attggattgg ccgtggcggc cgtgtacggg acaccacgca tgggccccgc
     6061 cttcggctcg atgttattgt ccggtcgtta cggcgcagag ttgatcaaga agaaattgaa
     6121 gcaggagtag cggagaacca ctattcccgc ggctcttcaa acccgtcact cagcaaggcc
     6181 gcagggagaa agacccccga ggcgtactct taatcgtacg tcgagggggg agagcgactg
     6241 agaacgatgc tggtggcggg tttcaagagc cgtatcatgg atgtgcagga tttcttagta
     6301 caaggcgaag gcagcgaaga agacaagcgt gaggcgtggg agctctttca acaagcctat
     6361 caaaggcaga tgaaaggcga attggaagaa gcagtctcgc tttaccgaca gtccatcgcg
     6421 acccatccga cggccgaagc tcatacgttc ctcggctgga cctatagctt tatgggccga
     6481 ctcgacgacg cgatcgagga gtgtcatcgc gcaatcggcc gcgatcccga gttcggcaac
     6541 ccctataatg acatcggcgc ctatttgatc gagaagggtg aactggacga cgccatcccg
     6601 tggtttcaaa aagccatgca tgcgaaacgg tacgagagtc cggcgttccc ccatttaaat
     6661 ctggggcgcg tgtatgagaa gaagggtaac tggacggaag ccatctcgtc gtacaaacag
     6721 gcattggcgt taaatcccga ttatgtgctg gcgaagaaat cgctggggcg attggtgagc
     6781 atgttgaatt gatgattgcg cttaccgctt tggccgcacg cagaccatga ctcacatcca
     6841 ttcgaaaacc atactggtcg ccgtcacgga catctttttc tatacgaaag tccgggatgc
     6901 cctgttgccg aacgggtata cgctggagag agcacgatcc catgaggaca ttgcagccag
     6961 ggcttcgacg acaccaccgg ttgccgtgat tctgaacatg aatgacgaaa cgttgaacgc
     7021 gtttcaggtc ttggagacgc tgaaggccga tccgcgattc catgcgcttc cgattttggc
     7081 ctttgcgaac catgaagagg tggatacgtg gaatcgggcc aagtccatgg gcgtgacgaa
     7141 aattgtgtcg cgaaatgagt tttccatgcg gaccagagac ctcgtggaag gggtcatctc
     7201 ccacatccca tcgtaaggct acggggacct cgatgaccac caatactgtc atgaaacact
     7261 cccgcttgca tgaccatctc gccaaactcg gcgcaacgtt cgaagcagtc acaggctggt
     7321 tgatgccggt tcactatggc caatgggtgg acgagtaccg tgcggtccga gagggcgtgg
     7381 gcctcgccga tctttcacat cgcggcaaac ttcgcgtcac cggggacgat cgcgtcaaat
     7441 ggctccaaag cgtaatcagc aatgacattc ttccgttgca gccgggacaa ggccgctact
     7501 ccagcctcct cacccacaag ggaaaaatgc tgacgtactt ccgctgctac atgcagaccg
     7561 acgcggtgat actggaggat gccggtgaga tcggtgatca gacctatgca gcattacgga
     7621 aattccttct ctacggaaca aaagccaaac tcgagaactg ccgagagagc tggggtctgc
     7681 tattggtcag cggcccaaag gcagcgacgg tggtaaaggc ggcgtttgga accgaccttg
     7741 ccgatctgaa acccgtcaac ttcattcccg ctgccatcgg cggccaaacg gcgctcatta
     7801 tccgttcgga agagacagga gaacaggatg tcgaaatcct
//
LOCUS       DASTQY010000068         3960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_354823, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000068 DASTQY010000000
VERSION     DASTQY010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354823"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VFR82_02140"
     CDS             complement(<1..522)
                     /locus_tag="VFR82_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU4500430.1"
                     /translation="MRDQHTFYITTPIYYVNDVPHIGHAYTTIAADVLARYWRLRGRD
                     VFFLTGLDEHGQKVQQAAAKAGIEPQAYCDRLAPQFDALWKRLNVTNDAFIRTTEAEH
                     KIIVQRYLQQLFDKQLIYKDSYTGWYCTFDERFWTEKDVVDSLCPDCKRPVERLSESN
                     YFFKMGQHQDSLIK"
     gene            complement(584..1609)
                     /gene="holB"
                     /locus_tag="VFR82_02145"
     CDS             complement(584..1609)
                     /gene="holB"
                     /locus_tag="VFR82_02145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU4500431.1"
                     /translation="MPFGSIIGHHSTIASLQTAVSRQRLAHAYLFHGENSIGKRTTAV
                     SFAQALNCDSAEQTRIDGCGVCRSCQQIEARTHPDYFVIEPDRELATPQIKIEQVREI
                     EQQIVYRPLVGERKICLIDDADRLTISAANALLKTLEEPPDHSLFLLITSRPASLPLT
                     IRSRCQSLRFATPARTDVEAALILKREIPPSDARLLAMISEGRLGEALSTDLKQVRTE
                     QQELLELMSPNSLQSITALLAAAESAAKADRATDLLSWLARWIRDLVIIQVGGDRDQL
                     LHLDHVPMLERYARQAQTGALLSVLSDIDHTLRQATRHLNLQMALENILFRLRDAIMT
                     SVPQPVS"
     gene            complement(1616..2290)
                     /gene="tmk"
                     /locus_tag="VFR82_02150"
     CDS             complement(1616..2290)
                     /gene="tmk"
                     /locus_tag="VFR82_02150"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU4500432.1"
                     /translation="MERPGKRLLSRGVFLTLEGPEGSGKSTQIAFLGTALRKAGYSVA
                     YTREPGGTKLAETIRGTLLSASSEEPIAPETEAFLVLAARSQHVTHFIRPALAKGSIV
                     LCDRFADSTFAYQGFGRQLSLSWLQTANHAATSGLMPHLTLLLDVPVSVGLARRRQAS
                     GKPNRLDQESKQFHMRVRKGFLTLASRTPRRIKVINANRPAPMVRAEIEAVVLGWLRK
                     RHRRGG"
     gene            2419..3087
                     /locus_tag="VFR82_02155"
     CDS             2419..3087
                     /locus_tag="VFR82_02155"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEU4500433.1"
                     /translation="MLKASLKRYFLTGLLVITPIWGTFLILKTLFVTVDGILGDLLAQ
                     LVPNHYVPGLGIVTLILLVFITGLLAANFIGRQIVKLWEGWLNRVPLVRGIYSTLKSM
                     MDILSFSDRGSYHRVVLIQFPKNGHYCFAFVTGVSKGDMQDLAQEPLVHVYVPTSPNP
                     TSGYFLLVPEREVTAVNISVEEAMKLIVSGGLYSPSSSLSTSLDPEAKWGAVKQPEAG
                     VPIG"
     gene            3042..>3960
                     /locus_tag="VFR82_02160"
     CDS             3042..>3960
                     /locus_tag="VFR82_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU4500434.1"
                     /translation="MGRCQAAGSGSADRMSRLPRQDATRNHEPRETARPVSGLAAIIM
                     AAGLGKRMHSKHAKVLHPVAGQAMVLYSVQLALRVADHHIAIVVGHQADRVSQVIKEA
                     TGERNGHTPVSIVEQRELLGTGHAVLQTRPVFLESGRFTPGRFLILNGDTPLLKETTL
                     RRLLAVHQEQQAAVTILTAVLDDASGYGRVVRQTSTDHVTRIVEERDADAATKAIHEI
                     NVGTYVVEGNLLFSALEKIQPSNAQGEFYLTDIVHLAVEEGRPVAAVLLDDPHEGLGV
                     NTRRQLAEAEQVVRKQIRDRWLDAGVRMID"
BASE COUNT          867 a         1058 c         1146 g          889 t
ORIGIN      
        1 tttgatcaga ctgtcctggt gctggcccat cttaaagaaa taattggact cgctgagccg
       61 ttcgacaggg cgcttacagt cggggcagag actgtcgacg acgtcctttt cagtccagaa
      121 ccgttcatcg aaagtgcaat accaccctgt atatgaatct ttatatatca gttgcttgtc
      181 gaacaactgc tgaaggtatc gttgaacaat tattttgtgc tcagcttctg tcgttctgat
      241 aaaggcatca ttggtcacat tcaggcgttt ccagagcgcg tcaaattgag gtgccagcct
      301 gtcgcaatag gcttggggct cgatgccggc cttggcggcg gcttgctgga ctttctgacc
      361 atgttcgtca aggccagtaa gaaaaaagac gtcgcgccct cgaagtcgcc agtaccgagc
      421 gagcacatct gcggcgatcg tcgtgtaggc atggccgatg tgcgggacgt cattgacgta
      481 ataaatcggc gtggtgatgt aaaaggtatg ctgatcgcgc atggtcagat ttcaacctta
      541 acagtcgaag aggacgaaaa ccaacgcctg gtattgactc gtctcacgaa actggctggg
      601 gaaccgacgt catgatcgca tcgcgcaggc gaaacagaat gttctcaagc gccatctgca
      661 aattcagatg cctggtcgcc tgtcgtaggg tgtgatcgat gtcgctcagc acggagagca
      721 gtgcgccggt ttgcgcctgt ctggcatacc gttccaacat aggtacgtgg tcgaggtgga
      781 gcagttgatc gcgatcgccg ccgacttgaa tgatgacgag atcacggatc cagcgggcca
      841 gccatgaaag gaggtccgtc gctcggtcgg cttttgcggc ggactctgcc gcggccagca
      901 gcgcggtgat tgattgtagg gaattcggac tcatgagctc aagcagttcc tgttgctcgg
      961 tacgaacctg tttcaagtcg gtcgacaggg cctctccgag gcggccttcg ctgatcatcg
     1021 caagcaatcg ggcatctgac ggcggtatct cgcgcttgag aatgagagcc gcctcgacat
     1081 cagtccgcgc cggcgtcgca aagcggagag actggcatcg cgaacggatc gtgaggggca
     1141 acgaggcggg tcggctggtg atgaggagaa agaggctgtg atccggtggc tcttcgagtg
     1201 ttttgagcag ggcattggca gcgctgatgg ttaaacgatc ggcgtcatct atcaggcaaa
     1261 tcttccgttc gccgacgaga ggtcgataca cgatctgctg ttcgatctct cggacctgtt
     1321 caatcttgat ttgaggagtc gccagttccc tgtccggttc aatcacaaag tagtccggat
     1381 gggtgcgggc ctcaatctgt tggcatgacc ggcacacgcc gcagccatcg atccgggttt
     1441 gctcggcact gtcgcagttg agggcctgcg cgaaagacac ggccgtggtt cgcttgccga
     1501 tagaattttc gccgtgaaac aggtaggcat gggcgagccg ctggcggctc acagcagttt
     1561 gcagagatgc gatcgtcgag tggtggccga taatactgcc aaacggcatg cgctcttatc
     1621 cccctctacg atgtcgtttt cgtagccagc ctaacacaac agcttctatc tccgctcgaa
     1681 ccataggtgc cggccgattt gcattgataa ccttaattct gcgcggtgtt cgtgacgcaa
     1741 gggtgagaaa tcccttgcgg acgcgcatat gaaactgttt ggactcttga tccaaccggt
     1801 tcggtttgcc ggaagcttgc cgtcgtcgtg ccaaacccac cgacaccgga acatcgagga
     1861 gcaaggtgag gtgcggcatc agaccgctgg tggctgcgtg attggcagtc tgtaaccatg
     1921 aaagcgaaag ctgacggccg aagccttgat aggcgaaggt ggaatccgca aaacgatcgc
     1981 ataaaacaat cgagcctttt gcaagagcag gccggatgaa gtgagtgaca tgctggcttc
     2041 tggcggcaag aacaagaaac gcctcggtct caggcgctat tggttcctcg gatgaagcgg
     2101 aaagaagggt tcctcggata gtctcagcga gcttcgttcc cccaggctca cgggtataag
     2161 cgaccgaata tccggccttg cgaagagcgg taccaaggaa agcaatttgc gtacttttcc
     2221 cactgccctc cgggccttcg agtgtgagaa acaccccccg cgacagaagc ctcttccccg
     2281 gtcgctccat aactcactcc agtgcgactg aaaaacgggc gggatagtag tccaagttat
     2341 tgaaaatagc aataaaaccc ttgcgagcgc gccagcaata tctgtatgat gagcagcaat
     2401 ccctgtctac agctatttat gttgaaagcc tcgctaaaac gctactttct gaccggtctc
     2461 cttgtcatca cccccatctg gggaaccttt ctcattttaa agactctttt cgtcaccgtg
     2521 gatggcatcc ttggagacct tctcgcgcag ttggtcccga atcattatgt tccgggtttg
     2581 ggaatcgtca cgctgatcct cctggtgttt attaccggac tgcttgcggc caatttcatc
     2641 ggaaggcaga tcgttaaatt gtgggaaggg tggctcaatc gagtgccgtt ggtgcgcggg
     2701 atctactcga cattgaaatc catgatggac atcctgtcct tctcggatcg cgggtcctac
     2761 caccgcgtcg tgctcattca atttccaaaa aacggacatt attgctttgc atttgtgaca
     2821 ggcgtgtcga agggcgatat gcaagatttg gcgcaggaac cacttgtgca tgtgtacgta
     2881 ccgacctcgc cgaatcccac ctccgggtat tttctcctgg ttcccgaacg ggaggtcacc
     2941 gctgtcaaca tcagtgttga agaagcgatg aagctgatcg tgtcaggcgg actctattca
     3001 ccatcctcct cactgagcac gtcattggat ccggaagcca aatggggcgc tgtcaagcag
     3061 ccggaagcgg gagtgccgat cggatgagcc ggctaccgcg ccaggacgca accaggaatc
     3121 atgagccgcg agagacggcc cgtcccgttt caggtctggc ggctattatc atggctgccg
     3181 gcttgggaaa acggatgcat tccaagcatg ccaaggtcct gcatccggtc gcgggacagg
     3241 cgatggtctt gtactcggtt cagttggctc tgcgcgtcgc cgaccatcac atcgccatcg
     3301 tggtgggtca tcaggccgat cgcgtcagcc aggtcatcaa ggaggcaacc ggagaaagaa
     3361 acggacacac acccgtttca atcgtcgaac aacgggaact gttgggaacc ggccatgccg
     3421 tcttacagac gcgacctgtg tttctcgagt ctggacggtt cacacctggg cgctttctca
     3481 ttctcaatgg cgataccccg ttgctcaagg aaacgaccct gcggcgacta ctcgccgtgc
     3541 atcaggaaca acaggccgcc gttaccattc tgacggcagt ccttgacgat gccagcggtt
     3601 acggccgcgt ggtgagacag acctcgaccg accatgtcac gcggatcgtg gaagagcgtg
     3661 acgctgacgc agcgaccaaa gccatccatg agattaacgt cggtacgtat gtcgtcgaag
     3721 ggaatttgtt gttttctgcc ctcgaaaaaa tccagcccag caatgcgcag ggtgaatttt
     3781 atctcaccga tatcgtgcac ttggcggttg aggaagggcg tccggtcgcg gcggttctcc
     3841 tcgatgaccc ccatgaaggt ctcggtgtca acaccaggcg gcagttggcc gaggccgagc
     3901 aagtcgttcg aaagcaaatt cgtgatcgtt ggctcgacgc cggggtgcgg atgatcgatc
//
LOCUS       DASTQY010000069         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1362816, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000069 DASTQY010000000
VERSION     DASTQY010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362816"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..850)
                     /locus_tag="VFR82_02165"
     CDS             complement(41..850)
                     /locus_tag="VFR82_02165"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEU4500435.1"
                     /translation="MATYAIGDIQGCYKSLHRLLDQIAFDPRSDTLWLVGDLVNRGPD
                     SLLTLRFLKSLGSSVRPVLGNHDLFLLAAAEGIVTLRPKDTIQDVLSADDCSDLTNWL
                     RRQPLHQREGSFFMVHAGLHPRWSIEQAMSFAKEVEGILAGSDYKTLLQELFHGSTPQ
                     WDPALKGTERLVAIARVFTRLRTISLAGEMSSFSGPPEDAPPGFMPWFRIPDRRSNDC
                     TIITGHWAALGLHIEPNILTIDSGCVWGRQLTAVRLEDRAVYQVEYADRRD"
     gene            complement(935..1330)
                     /gene="queF"
                     /locus_tag="VFR82_02170"
     CDS             complement(935..1330)
                     /gene="queF"
                     /locus_tag="VFR82_02170"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEU4500436.1"
                     /translation="MKKKKTGLGYNEQHARSGITAPLPDIETFPNQYKGYEITIEIPE
                     YTAICPKTGLPDFGTITVHYMPDRECLELKSLKMYIHAYRNIGIFYENSVNRILQDIA
                     KACRPVWARVTGTFAARGGLRSVIEARYP"
     gene            complement(1375..1623)
                     /locus_tag="VFR82_02175"
     CDS             complement(1375..1623)
                     /locus_tag="VFR82_02175"
                     /inference="COORDINATES: protein motif:HMM:NF023646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEU4500437.1"
                     /translation="MPIRVVIAGEDEDARHVGGVHKRPPIPDNSLKAECPKFMAHGPC
                     GGVRKGGFCEVYPEMTCPWITMFIELEKIGHTDWMKQV"
     gene            2008..>2412
                     /locus_tag="VFR82_02180"
     CDS             2008..>2412
                     /locus_tag="VFR82_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin oxidoreductase"
                     /protein_id="HEU4500438.1"
                     /translation="MSLDYVKFSSGFEKFMPKEYRDMVEHGPFGKKVSVSQMGSFKEV
                     LEEHPMCAGCAMTLFIRLAMIAFPNPEDTITVGTAGCGRLAISQAAIPFVYGNYGDQN
                     GVASGLSRGLRLRFGDKPKDVVVMAGDGGTADI"
BASE COUNT          521 a          633 c          691 g          567 t
ORIGIN      
        1 atgagagtag cgaggccacg acgggtccgg agcttggggc tcaatcgcgt cgatccgcat
       61 actcaacctg atacacggcg cggtcctcca agcgcactgc cgtaagctgt cttccccata
      121 cgcacccact gtcgatcgtg agaatgttcg gttcgatatg aagacccagt gccgcccaat
      181 ggcctgtgat gatggtgcag tcgttgctgc gacgatcggg aatgcggaac cagggcatga
      241 aacctggtgg ggcgtcctcc ggagggccgg aaaagctcga catctcaccg gccaaggaga
      301 tggttctgag tcgcgtgaac acacgcgcta tagcgaccag tcgttccgtt cccttgagtg
      361 ccggatccca ctgcggagtc gaaccgtgaa aaagttcctg caacaacgtc ttgtagtccg
      421 accccgccaa gatcccctcc acctcctttg cgaagctcat cgcctgctct atcgaccatc
      481 gcgggtgcag acccgcatgc accatgaaga aggatccttc gcgctgatgc aggggctgcc
      541 gtctgagcca attggtgagg tcagagcagt cgtcggcgct caagacatct tgaatggtat
      601 cctttgggcg gagtgtcaca atgccctctg cggctgccag taaaaataga tcgtggtttc
      661 cgagcacggg ccgcaccgat gagccgagag atttaaggaa tcgaagagtc agcagggagt
      721 ccggtcctcg attgaccaag tcaccgacca accaaagcgt gtctgaccgt ggatcaaacg
      781 cgatctggtc gagcagtcga tggagagatt tgtagcagcc ttgaatgtcg ccgattgcgt
      841 aggtggccat gaataccaag cgtcggtgtg gtcagaaact ggcaggacgg tgagcggtca
      901 tgtcggccct gcggctactg acgatctcgt gtggtcaagg ataccgggct tctatcacgc
      961 tacggagtcc tccgcgggcc gcgaacgtgc cggtgactcg cgcccaaaca gggcggcagg
     1021 ctttggcgat atcttggaga atccgattga cggagttctc gtagaaaatg ccaatgttgc
     1081 ggtaggcatg aatatacatt ttgagtgatt ttagttcgag gcattcacga tccggcatgt
     1141 aatgcacggt aatcgtgccg aagtccggga gccctgtttt gggacaaatc gccgtgtact
     1201 cagggatctc gatcgtgatc tcatagccct tgtactgatt ggggaacgtt tcaatatcag
     1261 gaagcggagc cgtgattccg cttctggcat gttgctcgtt atagccgagg ccggtcttct
     1321 tctttttcat tctcgtatta ttaaggtgag cgatgcgaag atgagcacga ggcgtcacac
     1381 ctgcttcatc cagtccgtgt ggccgatctt ttccagttca ataaacatgg taatccatgg
     1441 gcaggtcatt tccgggtaga cttcgcaaaa gccacctttc cggacgcctc cgcaagggcc
     1501 atgagccatg aattttgggc attcggcctt caacgagttg tccgggatcg gcggtcgctt
     1561 gtggacgccc ccgacgtgtc gggcatcctc gtcttcgccg gctatgacga ctcgtatagg
     1621 cataacttcg ctagtgtaga cagatttttt gccaagggca acgtacattt gccttgtggt
     1681 tcgtgtcgtc ccaatcagaa gccgtctcaa ggttagggcg aatggcctat cgcattaggg
     1741 aagagctagg tcttctttcg gacagggtgg gccttctgca aagttgcatt gaatatcagg
     1801 aagttataaa attttcgagg tgagccgaca atcctcgtcg cgagaacata taaattgaca
     1861 gtgttttttg agctttgtta ggatggccgc gacgcgaaca tgagccggtg gacgtgtcgc
     1921 ttccgttttc tttcccgctg atcgtcttac tttacactga atgaatggcg accttgtccg
     1981 tcgcagtgac ataaagggag gtgtccgatg agtctcgatt atgtaaagtt ttcaagcggc
     2041 tttgaaaagt tcatgccgaa agaatatcgg gacatggtcg aacatggacc cttcggaaag
     2101 aaggtgtccg tctcgcaaat gggcagcttc aaagaagtcc tcgaagaaca tccgatgtgc
     2161 gctggctgcg caatgacgct gtttattcgc ctagcgatga tcgcgtttcc caatcctgaa
     2221 gacaccatta ccgtcggcac cgccggctgt ggccgcttgg cgatttctca agccgccatc
     2281 cctttcgtat acggcaacta tggcgaccaa aacggtgtag ccagcggact ttctcgcggt
     2341 cttcgccttc gcttcggcga caagccgaaa gacgtggtcg tcatggccgg tgatggcggc
     2401 accgccgaca tc
//
LOCUS       DASTQY010000070         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1765810, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000070 DASTQY010000000
VERSION     DASTQY010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765810"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..474)
                     /locus_tag="VFR82_02185"
     CDS             complement(61..474)
                     /locus_tag="VFR82_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500439.1"
                     /translation="MTDIVKPSIQAFLVSDQVIEDSVTKKKSLIGLFTHLQAVSFPFQ
                     HQQMGLYFCLTDAEGAYQFDIELIYLNTEQLVCRATLPRIVIGDRLQISDFGINIPSL
                     IFPAPGRYEFRLRMEGHLIAQKDFNVMLLSTHQTA"
     gene            complement(561..1106)
                     /locus_tag="VFR82_02190"
     CDS             complement(561..1106)
                     /locus_tag="VFR82_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500440.1"
                     /translation="MNNPFGTDADMMDLGDTAELIVEEEKPEQGLELHVGSNIFRTTN
                     GVVKLQGKEQIVIEVQPDPPALLLTMDFYDERGQRIGHIRRNQLSVVGSTRFALCVTT
                     ASDATLDDPLTVSVSDRATDRTVIEVSLFQRRKIRFMSAYFHTHKGEVVSITPHYCRI
                     GSGLTMFGDVVEGRGGSAMLG"
     gene            1288..1365
                     /locus_tag="VFR82_02195"
     tRNA            1288..1365
                     /locus_tag="VFR82_02195"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1324..1326,aa:Glu,seq:ctc)
     gene            1589..2212
                     /locus_tag="VFR82_02200"
     CDS             1589..2212
                     /locus_tag="VFR82_02200"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4500441.1"
                     /translation="MKSGRVVASQYAMLLVVPVMCLMIFSCSGSKPVEVTGNDLSRLE
                     ETAKELRKMAQGGDASAQNELGLLYYEGRGVVQNYGKAKEWFDKAVEQGHTGAQVNLG
                     TLYLRGEGAPQSSQMALFWFTRAAGQEDALALAKLGLMYAQGRGVLQDFIQAHMWYNL
                     SAARGEARSAEARDALAKQMTPTQIAEAQRLAREWRPREEKTRKTVY"
     gene            complement(2326..>2508)
                     /locus_tag="VFR82_02205"
     CDS             complement(2326..>2508)
                     /locus_tag="VFR82_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500442.1"
                     /translation="EEPHDAAKQLEWTPWIVQGRPLAKMTAHDDQQDFGWEFAYSLRV
                     EPASSRRQHSIARYVE"
BASE COUNT          595 a          661 c          688 g          564 t
ORIGIN      
        1 accataagaa ccgtgcgggg gagaagagtg cggggatctc gaggtgccgg aataaaatgc
       61 tcaggcggtc tggtgggttg acagcagcat gacgttgaaa tctttttggg caatcaagtg
      121 gccttccatc ctgaggcgga attcataacg gcccggagcg ggaaagatca gcgagggaat
      181 attgatccca aagtcagaaa tctgcagacg atcaccgatg acaattcgcg gtagcgtagc
      241 cctgcacacg agttgctcag tgttaaggta gatcagctcg atgtcgaact gataagcgcc
      301 ttcggcatca gtgagacaaa aatacaaacc catctgttga tgctggaacg gaaagctcac
      361 ggcttgtaaa tgcgtgaaca aaccaatcaa actctttttc ttggtcacgc tgtcttcgat
      421 cacctgatcg gagacgagga aggcttggat gctcggcttg acgatgtccg tcatgagcgt
      481 attctacggg agtccgcccg gctgtcaaag ggataagttt ttgagggtat ttgctacggc
      541 aggaagaaag cccgctacga ctaacctagc attgcgcttc cgccgcggcc ttcgaccacg
      601 tcgccaaaca tcgttaatcc agagccgatg cggcaatagt gcggagtgat ggagaccacc
      661 tcgcccttat gagtatgaaa atacgcgctc ataaaccgta tcttccgacg ttggaacaac
      721 gacacttcaa tgaccgtacg gtcggttgct cggtcagaca cgctgactgt cagcggatcg
      781 tcgagcgtgg catctgatgc cgtcgtgacg catagcgcga agcgggtcga tccaaccacc
      841 gacaattgat tgcgtctgat atggccgata cgctgaccgc gctcgtcgta gaagtccatg
      901 gtcagtaata aggccggcgg gtccggctgc acttctatca caatctgttc ttttccttgt
      961 aacttcacga cgccattcgt cgtgcgaaag atgttcgagc cgacatgcaa ttccaagccc
     1021 tgttccggct tctcttcctc gacgataagt tcagcggtgt cgccgagatc catcatgtcc
     1081 gcatcggtcc caaacggatt attcatcgtc tcatgatcct agccggaagc aggtgaagta
     1141 gtctctcttg acttgcgaaa gttaggccaa gccaccagcg ctgtcaagat gctatttgcc
     1201 aaaaaaatac ctacctgccg ctgtgcgttt aaattgaaaa acccaagtgc ctcatggtac
     1261 cgtcagcgtt ccattcctta cgcctcgggt cccatcgtct agcctggcct aggacggagc
     1321 cctctcaagg ctcagacacg ggttcaaatc ccgttgggac caccagcacc tgtcttgcta
     1381 tctcctccac cctgtgcggc tctcgcgtcc aggctaagga agctatggat gtctcctggc
     1441 gaatacaaac ctacacctgg tcggcgctga ttccggtagc ttctagatga aattgatacg
     1501 tggcgggagg catggaggtg gctgcggagg catggagatt gctgcattga ctgcagtcta
     1561 acctcagctc aactagacgg agtcgtcaat gaagagcggt cgggtggttg catcacaata
     1621 tgccatgttg ctcgttgtcc cagtaatgtg tctgatgatt ttttcctgtt ccggcagtaa
     1681 gccggtcgaa gttacgggca acgatctttc gcgtttggaa gaaacggcga aggagcttcg
     1741 caaaatggct caaggaggag atgcatcggc tcaaaatgaa cttggtctgt tgtactacga
     1801 aggcaggggc gttgttcaga actacgggaa agcgaaagag tggttcgata aggctgtaga
     1861 gcaggggcat acaggagccc aagtcaatct cgggacgttg tatctccgcg gggaaggcgc
     1921 tccgcaaagt agccaaatgg cattgttctg gtttacgcga gcggcaggac aagaggatgc
     1981 tttggcgtta gccaaactgg ggttgatgta cgcacaggga cggggagtgc ttcaagactt
     2041 catccaagcc cacatgtggt acaacctgtc ggcagcccgt ggagaagcaa ggtcagcgga
     2101 agctcgtgat gctcttgcta agcagatgac gccgacccag attgccgaag cacagcggct
     2161 ggcccgcgaa tggaggccaa gagaagagaa aacacgaaag accgtctact agtcactaca
     2221 ccatccgaac catacccaat ttcgccagaa acctactgcg gaacaccatt cctgacgcct
     2281 cacctcctcg cttctcacgc ctcggtggat gtcccgcctc gaatctcatt cgacgtatct
     2341 cgcaatggaa tgctgtctgc gggatgacgc gggttctact cgaagtgagt aggcgaactc
     2401 ccagccgaaa tcttgctgat catcgtgcgc tgtcattttc gccaacggcc tcccctgcac
     2461 gatccatggt gtccattcaa gctgtttggc tgcatcgtgc ggctcttc
//
LOCUS       DASTQY010000071         9216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_958139, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000071 DASTQY010000000
VERSION     DASTQY010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9216
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958139"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..523)
                     /locus_tag="VFR82_02210"
     CDS             complement(167..523)
                     /locus_tag="VFR82_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEU4500443.1"
                     /translation="MDSTKTETQGPVISLTDAAIKEVKRLINVQGITEGGLRLGVKGG
                     GCSGLSYTVNFDEKIGQYDQVYDFDGVKVIVDAKSAIYLQGTQLDYQKDLMGGNFKFV
                     NPNANKTCGCGESFSA"
     gene            complement(541..924)
                     /gene="iscU"
                     /locus_tag="VFR82_02215"
     CDS             complement(541..924)
                     /gene="iscU"
                     /locus_tag="VFR82_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEU4500444.1"
                     /translation="MAYSDKVVDHFNNPRNMGSFKKEEEGVGTGMVGAPECGDVMKLQ
                     IKVQNDTIVDAKFKTFGCGSAIASSSLATEWLKGKTLEEAQKIKNTDIVQELNLPPVK
                     IHCSVLAEDAIKAALADYQKKSETK"
     gene            complement(976..2193)
                     /locus_tag="VFR82_02220"
     CDS             complement(976..2193)
                     /locus_tag="VFR82_02220"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEU4500445.1"
                     /translation="MKFPIFLDNHSTTPMDPRVLEAMLPYFVEKFGNAASRNHAFGWA
                     AEEAVETARKQIARLIKADPKEIVFTSGATESDNLALKGVLEMYKEKGNHIITSATEH
                     RAVLDTAKSLEAKGLATVTYLPVDKQGMVNPEDVRKALTEKTVLISVMLANNEIGTIN
                     PIQDIGKIAKERGVLFHCDATQGVGKIPVDVQAMGIDLMSFSAHKIYGPKGVGALYVR
                     KKNPRVRIAAQIDGGGHERGMRSGTLPVPLIVGFGKACELCEQEMPAESARLANMRDR
                     LQAGIMAALEESYLNGHPTNRLPGNLNISFAYVEGESLLMGMKEIALSSGSACTSATL
                     EPSYVLRALGVGTELAHSSIRFGLGRFNTDEEIDYTIKKVIEIVTKLREMSPLYEMAK
                     EGVDLKSVQWAAH"
     gene            complement(2190..2663)
                     /locus_tag="VFR82_02225"
     CDS             complement(2190..2663)
                     /locus_tag="VFR82_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU4500446.1"
                     /translation="MLKISKKTDYALMALQHIASVQFGDLTPSRVVNTKEIAEEYNIP
                     LELLAKVLQSLSKSGMIESHNGPKGGYHLARKAREITIAQILESIEGPLGITDCSHEK
                     EGEFCMQREHCNIRTPLLKVQDSIYQLLNNMTLQDMLGGTPLITIQSPTPQGAER"
     gene            2783..3199
                     /locus_tag="VFR82_02230"
     CDS             2783..3199
                     /locus_tag="VFR82_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU4500447.1"
                     /translation="MGGTNPYIEKADVELPKEPYTVTYIAPSGTVTKVQVDPAKIPYG
                     QTGLPGSLLDIAMANGVDLEHVCGGVCACSTCHVIVRQGLETCNEGTDDEFDQLDEAP
                     MTTLQSRLGCQCVPNGTKDVVVEIPAVNKNLVKEGH"
     gene            complement(3245..3949)
                     /locus_tag="VFR82_02235"
     CDS             complement(3245..3949)
                     /locus_tag="VFR82_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4500448.1"
                     /translation="MAEFIQPATVLSSTNLTPHVRQLVILPKERPIVFKAGQWVSLKL
                     PVGTKPPLNRAYSMAAPGTTSGELILVLDRVPGGLGSNYLFMAKPGDEIILSGPYGSF
                     LLPPHVDRELLFVTRYTGLVPIRCMLKHLYAERYRGPVLLIAVAPEEQELLFHQELLT
                     LAVTHPGFRYLPLVAAGGEQQAVDLTLPMLRPLLEASPQVIPMLCGTKAFVRPLRAHF
                     VEAGYDRKEVKTETYD"
     gene            4077..5498
                     /gene="gltX"
                     /locus_tag="VFR82_02240"
     CDS             4077..5498
                     /gene="gltX"
                     /locus_tag="VFR82_02240"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU4500449.1"
                     /translation="MTQVRVRFAPSPTGFLHIGGVRTALFNWLYARQEHGVFILRIED
                     TDQSRSTDESIQAIVEGLQWVGLDWDEGPYRQTERMNLYREHAMALFERGQAYWCVCK
                     AEELEARRKEAEAKGLSPRYDGRCRTLGIIRPSGDAALRFKAPQEGQTVVDDLIKGKV
                     VFENNVLDDLIILRSNGYPTYNFSVVVDDALMHITHVVRGDDHLTNTPRQIPIFEALG
                     FPVPRFGHLPMILGADKTRLSKRHGATSIMAYKTMGYLPDAMVNYLVRLGWSYGDQEI
                     FTRQELIERFSWKHVQTSAAVFNPDKLLWMNAEYIKTSPPNEVAKALVPYLEQTDLKD
                     HVQAVPEEWLAQLVVLVRERTKTVVEMVDWVKPYFGETVALEDEAAKKYLTQSVAPVL
                     KKLTERIGSLPEFSKPALEACFKALVEEERLKMGQLAQPVRVALTGRTASPGLFEVME
                     ILGPERTRLRLRQGIERAEARPA"
     gene            5535..5608
                     /locus_tag="VFR82_02245"
     tRNA            5535..5608
                     /locus_tag="VFR82_02245"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5567..5569,aa:Gln,seq:ctg)
     gene            5651..5724
                     /locus_tag="VFR82_02250"
     tRNA            5651..5724
                     /locus_tag="VFR82_02250"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5683..5685,aa:Gln,seq:ttg)
     gene            complement(5740..6543)
                     /locus_tag="VFR82_02255"
     CDS             complement(5740..6543)
                     /locus_tag="VFR82_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU4500450.1"
                     /translation="MAKKQRRLRANKAKTGNKDSDENGMGQRRTLVVDVGGSGIKATL
                     LNDLGKAVGDRLRRDSPSSGIPSEVMDIIVSLSKQFESFDRVAVGFPGVVVNGIVKQA
                     PNFAKEWQDYNIAEVLEARLKKPVRVANDADVQGFGAISGTGVELVVTLGTGVGTSLF
                     VDGRLVPNLEIGRDKLRDAALQKVGKKKWNKRLVKFIKRLDEIFHFTKMYIGGGNSRA
                     VDVGMLPANVTIISNLNGLIGGIALWRDERVSSVEVASQPDITGRPLAA"
     gene            complement(7011..7346)
                     /locus_tag="VFR82_02260"
     CDS             complement(7011..7346)
                     /locus_tag="VFR82_02260"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4500451.1"
                     /translation="MIECRQYPRVPVDLQVYFSTTGNTSIREGTMFDLSAGGCAVTSM
                     SAVRPGSAVRILIRATDLGSPITIESAAVRWSKHGEFGVEFLGVSELDQNRLHRLLHA
                     KTGYPLLPS"
     gene            complement(7513..>9216)
                     /locus_tag="VFR82_02265"
     CDS             complement(7513..>9216)
                     /locus_tag="VFR82_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEU4500452.1"
                     /translation="GETVPDGTTASNFIREIVSADRAAGKHGGWVVTRFPPEPNGYLH
                     IGHAKSICLNFGLAQDHLGGKCHLRFDDTNPTTEDPQYVQAIQEDVRWLGFDWGENLF
                     FASDYFERLYTLAEGLIRKGLAYVDSLSPEDIRRYRGTLTEPGTNSPYRDRPAEESLD
                     LFRRMRAEEFADGTHVLRAKINMASPNMNLRDPILYRIRHAAHYRTGKTWCIYPAYDY
                     AHPLSDAFESVTHSICTLEFEDHRPLYDWVVERADTEARPQQIEFARLNISQAVMSKR
                     KLLELVEKKLVAGWDDPRLLTIKGLRRRGYTPEAIRAFCDHIGVSKRDSVVEMQLLEH
                     FIREDLNKRAPRVMAVLKPLKVIIDNYPDGVTEQLEAINNPEDPSAGTRPLPFSRTLY
                     IERDDFLEDPPKQFFRLAPGREVRLRYAYIIKCVGVMKDAGTGEITELHCTYDVETKS
                     GAPSAQRKVKATIHWVSAAHAVDAEVRLYNPLLKGELFSLEDQQDWTTHLNPQSLERL
                     LGCRVEPSLKEAQPGNRFQFERLGYFCMDADSVTDRLVFNRTVSLKDAWAKLEKSGSS
                     R"
BASE COUNT         1999 a         2501 c         2543 g         2173 t
ORIGIN      
        1 ggccacctcg gattggcagt gccagcacat gctcctggcc atctgcagct cccgccgccc
       61 tgaattgtca atgcctgatt gatgatccat ggtcacatgc tgatctgaaa aaacaggcat
      121 ggcggatgcc atgcctgatt gaagaaactc ggacttggct tcaagcttat gccgagaacg
      181 attctccgca gccgcaggtc ttgttggcgt tgggattgac gaacttgaag tttccgccca
      241 tcaggtcctt ctgataatcg agttgggtgc cttgaaggta gatggcactt ttggcatcca
      301 cgatgacctt cacgccatca aaatcgtaga cctgatcgta ttggccgatc ttttcgtcga
      361 aattgaccgt gtaactcagt cccgagcacc cgccaccttt gacgccgaga cgaagccccc
      421 cctcggtgat gccttgaacg ttaatcagac gtttcacttc cttgattgcg gcatcggtga
      481 ggctgatgac gggcccctgg gtctctgtct tcgtgctatc catgaggcgc tccttcctgc
      541 ttacttcgtt tcgctctttt tctggtagtc agcgagagcc gccttaatcg catcttcagc
      601 gagcaccgaa caatggatct tcacaggcgg caggttcaac tcctgcacaa tgtcggtatt
      661 cttgatcttt tgagcctctt ccagggtctt ccctttgagc cattcagtgg ccaggcttga
      721 actcgcgatg gcggatccgc agccgaacgt cttgaatttc gcgtcaacga tcgtatcgtt
      781 ctgcaccttg atttgcagtt tcatgacgtc gccgcattcc ggcgcgccga ccattcctgt
      841 gccgacgcct tcctcttcct tcttaaagct tcccatgttc cggggattat tgaaatgatc
      901 gacgaccttg tcactgtacg ccatagtacc ctccgcattt atgacatcta gaatgtaagc
      961 ttcttctctc ctctatcagt gggcagccca ttgaactgat ttcaaatcca cgccttcttt
     1021 tgccatttca taaagtggag acatctctcg cagcttcgtc actatttcaa taaccttctt
     1081 gatcgtatag tcaatttcct catccgtgtt gaagcgcccc aggccgaagc gaatggatga
     1141 gtgggcgagt tccgtcccga cgccgagggc gcggagcaca taggagggct ccagcgtggc
     1201 ggacgtgcaa gccgacccgg aggacaatgc gatttccttc attcccatca acaacgactc
     1261 accttcgacg taggcaaatg aaatgttcaa attgcccggc aaccgattgg ttgggtggcc
     1321 gttgagatag ctttcttcta aggctgccat gatccccgct tgcagccggt cacgcatgtt
     1381 tgccaagcgg gcggattcgg cgggcatttc ctgctcgcag agttcacagg ccttgccgaa
     1441 tcccacgatt aacggaacgg gcaatgtgcc ggagcgcatg cctcgctcgt gcccgccacc
     1501 gtcgatctga gcggcaatcc ggacgcgtgg attctttttt ctgacgtaga gcgcgccgac
     1561 acccttcggg ccatagatct tatgcgcaga gaacgacatg aggtctatgc ccatggcctg
     1621 aacgtcaacc gggatcttgc cgacgccctg tgtggcgtcg cagtgaaaaa gcacgccccg
     1681 ttccttggca attttgccga tgtcttgaat gggattgatc gtgccgattt cattgttggc
     1741 gagcatcacc gagatgagta ccgtcttttc ggtaagggcc ttccgcacat cctctggatt
     1801 aaccatccct tgcttgtcga cgggcaggta ggtgacagtc gcgagacctt tggcctcgag
     1861 tgatttggcc gtgtccaaga ccgcgcgatg ctctgtcgcg gacgtaatga tgtgattccc
     1921 cttctctttg tacatttcca gaacgccctt gagtgcgaga ttatccgatt ccgtcgcacc
     1981 gctggtgaag acgatttctt taggatcggc tttaatcagg cgggcaatct gcttgcgcgc
     2041 tgtttcaaca gcctcttcgg ccgcccaacc aaacgcatga ttacgactag ccgcattccc
     2101 aaacttctcg acaaaatacg gaagcatggc ctccaaaact cgtggatcca ttggcgtggt
     2161 ggaatgattg tctaaaaaga tcgggaactt catcgttcgg ctccttgtgg tgtcggggac
     2221 tgaatcgtaa tgagcggggt gccgcccagc atgtcttgaa gtgtcatatt gttcagcaac
     2281 tgataaatac tgtcttgcac tttcaggaga ggcgtacgga tattgcaatg ttcccgttgc
     2341 atgcaaaatt caccctcttt ttcgtgggag caatcggtaa ttcccagggg accctcgata
     2401 ctttccaaaa tttgggcaat ggttatttct cgtgccttgc gcgcaagatg gtaccctccc
     2461 ttcggcccgt tatgactttc gatcatgccg ctcttggaga gtgactgaag caccttggcg
     2521 agcagttcca acgggatatt gtattcctcg gcaatttctt tcgtattgac cacccgactt
     2581 ggtgtgaggt cgccaaattg gacggacgca atgtgttgaa gggccatcag cgcataatcg
     2641 gttttttttg aaatcttcag catgctagcc tattgccgat cactctgatc ggagactata
     2701 atgatctccg accaatctag tcggactaat agtagcacga ggaatttaga cctgtcaacg
     2761 caggctcgag aaaggaagag gcatgggtgg aaccaatccc tatatagaaa aagcggacgt
     2821 tgaacttccg aaggaacctt atacggtgac ctacatagcg ccaagtggca cagtcaccaa
     2881 agtacaggtc gacccggcga agatccctta cggccaaaca ggcctcccag gcagcctcct
     2941 cgacattgcc atggcgaatg gagtggatct agagcacgtc tgtggtggtg tctgtgcgtg
     3001 ctcgacctgt catgtcatcg tgaggcaagg attggagacc tgcaacgagg ggacggacga
     3061 tgaatttgat cagctggatg aggcaccaat gaccacgctt cagtcacgtc tgggttgcca
     3121 gtgcgttcca aacggcacga aggatgtcgt cgttgaaatt ccagccgtca acaagaacct
     3181 tgtgaaagaa gggcattagc tgcatcggag tcgcttccag gcttgttgaa cctctgcgcg
     3241 agctttagtc gtaggtttcc gttttcacct ctttccggtc atagcctgct tcaacgaagt
     3301 gagcccgtag cggcctcaca aaggcttttg ttccgcagag catcggaatc acttgtgggc
     3361 tagcctccag caagggacgc agcataggca atgtcaggtc tacagcctgc tgctcaccgc
     3421 ccgccgcgac caagggcaaa taacgaaaac cgggatgagt gaccgccaac gtgaggagtt
     3481 cctgatgaaa cagcagctct tgttcttcgg gggcaacggc gatcaggagt actggaccgc
     3541 gatatcgttc agcatacagg tgcttcagca tgcaacggat gggaaccaga cctgtataac
     3601 gcgtaacgaa caacaactcg cggtcaacgt gcggcggaag caaaaaactt ccatagggtc
     3661 ccgacagtat gatctcgtct ccgggcttcg ccatgaatag gtagttggag cccaatcctc
     3721 ctggtactcg gtcaagcacc aggattaatt cacccgaggt cgtaccggga gccgccattg
     3781 aataagctcg attgagaggc ggttttgtcc cgaccggtag cttgagtgac acccactgac
     3841 ccgccttgaa aacgataggc cgttctttcg gtaaaatcac cagttgacga acatgaggcg
     3901 tgagattagt cgaagacagg acagttgcag gttggataaa ttctgccatg gctaggccag
     3961 ttgtccccca gtcttatcag atgcccaggt tggaagaaag ggcgttccat cctgtacaga
     4021 cttgtggacg cgtgctatag ataccggccc tccaacacgg atctctgaga gtgattatga
     4081 cccaagttcg tgtccgtttt gcgcccagcc caacaggctt cctgcatatc ggcggtgtcc
     4141 ggacggcgct ctttaattgg ctctacgcga ggcaagagca cggtgtcttc atcctgcgca
     4201 ttgaagatac cgaccagagc cggtcgacgg atgaatccat tcaagcgatc gttgaaggat
     4261 tgcagtgggt ggggcttgat tgggacgaag ggccgtatcg gcagaccgaa cggatgaatc
     4321 tctatcgtga gcatgcgatg gcgctgttcg agcgaggaca ggcctactgg tgcgtgtgca
     4381 aggccgagga gctggaggcc cgcagaaaag aggcggaggc aaaaggcctg tcgcctcgct
     4441 atgacgggcg gtgccggacg ttggggatta ttcgtccttc gggagatgcc gcgctgaggt
     4501 tcaaagcacc acaggaaggg cagaccgttg ttgacgactt gatcaagggc aaagtcgtct
     4561 tcgagaataa cgtgctggac gacctgatta tcctgcgatc aaacggatat ccgacctata
     4621 acttctcggt tgtggtggac gatgcgttaa tgcacatcac ccacgtcgtc cgaggggatg
     4681 atcatctcac gaatacacca cggcaaattc ccatttttga agcgctcgga tttcctgtgc
     4741 cgcgcttcgg ccatctgccg atgattttag gcgccgataa gacgcggctc tctaagcggc
     4801 acggcgcgac ctccatcatg gcctacaaaa cgatgggcta cctgcccgat gcgatggtca
     4861 actatctggt ccgactcgga tggtcctacg gcgatcagga aatcttcacc agacaagaac
     4921 tgatcgagag attcagctgg aagcatgttc agacatcagc agccgtcttc aatcccgaca
     4981 agttattgtg gatgaatgcc gagtacatca aaacgagtcc gccaaacgaa gtggccaaag
     5041 cactggtgcc atacttggaa cagacagacc tgaaggatca cgtgcaggct gtgcccgaag
     5101 agtggcttgc gcagttggtg gtcttggtcc gggagcgcac aaaaacggtc gtggaaatgg
     5161 tcgattgggt gaaaccgtat tttggagaaa ccgtggcgtt agaagacgaa gcggcaaaaa
     5221 aatatttgac ccagtcggtc gcccctgtgt tgaaaaagct gaccgagcgt atcgggtccc
     5281 ttcccgaatt ttcaaaaccg gcattggaag cgtgttttaa agccctcgtc gaggaggaaa
     5341 ggctgaaaat ggggcagctc gcccagcccg tccgagttgc gctgaccggt cgtaccgcca
     5401 gtccgggact cttcgaggtg atggaaattc tcggccccga acgaacgcgg cttagacttc
     5461 gacaagggat cgaacgggct gaggcccgcc cggcttgacg gaccacgcgg acgtgtatta
     5521 cgttgaacgc cggttggggg atcgtctagc ggtaggacgt cagtctctgg atctgactac
     5581 ctaggttcga ttcctagtcc cccagccacc gtctcctgac attcaccgcc ttctcaaatc
     5641 tcggccgcgc tgggggatcg tctagcggta ggacgccagc ctttggagct ggctacctag
     5701 gttcgattcc tagtccccca gccacaatct cttcacagac taggcagcca acggacgtcc
     5761 ggtaatgtca ggttgggatg ccacttcaac tgatgagact cgttcgtccc gccacagtgc
     5821 aatcccacct atcaggccat tgagatttga aataatcgtg acattggccg gcagcatgcc
     5881 gacatctacg gctcgggaat tgccgccacc gatatacatt ttcgtaaaat gaaagatctc
     5941 atccaggcgt ttaatgaatt tcacaaggcg cttattccac tttttcttgc ccaccttttg
     6001 taaggcggcg tccctcagtt tgtcacgccc gatttcgaga ttgggaacga ggcgcccgtc
     6061 cacaaagagg gaagtcccca cgcccgttcc gagcgtcacg accagttcca ccccggtgcc
     6121 ggatatggcg ccaaagcctt gcacatcggc atcattggct acccgcaccg gctttttcag
     6181 tcgcgcttcc aatacttcag cgatattgta gtcttgccat tctttggcga aattcggcgc
     6241 ttgtttcacg atgccgttaa cgacgacgcc gggaaatccg actgctaccc ggtcgaacga
     6301 ttcgaactgc ttggaaagtg acacgataat gtccataact tcgctcggta ttcccgagga
     6361 gggcgagtct cggcgcagcc ggtcgcccac cgccttgccg agatcattga gcaatgtcgc
     6421 tttgattcca ctgcctccca cgtctaccac aagagtgcgg cgttgcccca tgccattctc
     6481 atcggagtct ttgttaccgg tcttggcctt attggcccgg agccgtctct gcttcttcgc
     6541 catacccatc cttactcccg gagacgactt gtctcagcgg ggatgatctg tcttcgttat
     6601 tcggctcgtt gacgtccaaa ccactgtcac agttcgtgtt tctcaacgct ccgtcgccgg
     6661 tctaggccgc ttgcaggact tacgccacgc tcttgaaatg aagggcgatg tgaaccaagg
     6721 cgtcaccctg aaatagtgga atggcctatt gagtcttaat taatggtgag tccggcgggc
     6781 tggatcttcg ggctcttctc cctcatcagt aggcagttcc atcatgaggt tagggcctgg
     6841 aaggcggtat tgctgggcgg cccaagatcc caggtcagtc agctggcagc gttcggaaca
     6901 gaaaggtcgc caggggttac cctcccaggg ggctggttga cagcagaggg ggcaagtcat
     6961 gccgacatct tatcatagcg gtgaggttgg ggcgccccgg tggaacctag tcagcttggg
     7021 aggagaggat agccggtttt ggcgtgcaac agacggtgca gacgattttg atcgagttct
     7081 gacaccccaa gaaattcgac gccgaattcc ccgtgcttgc tccaccggac agcggccgat
     7141 tcaatagtga ttggggatcc taaatccgtt gcgcgaatga ggattctgac tgctgagccg
     7201 gggcgcacgg cgctcatact ggtgacggca caaccgccgg ccgaaagatc gaacatcgtt
     7261 ccttcgcgga tcgatgtatt gccagttgtt gaaaaataga cttgcaggtc gaccggaaca
     7321 cgggggtact gccgacattc aatcattctg gggctccttg gttatcctag gctccagccg
     7381 atccgatgca taccggtctc tctcagaagc agtgacttac cggtatccca cctaagccta
     7441 gcagggatgc ttaatctgta aagtgaatgc aagctagcgg aactagagat gtttcccgtg
     7501 cacggggcga gattatcggg aggatccgct tttttcgagt tttgcccagg catccttcag
     7561 cgatactgtg cggttgaaca caagcctgtc ggtcacggag tcagcgtcca tgcaaaaata
     7621 gccaagacgc tcgaattgaa accggttgcc tggctgggcc tccttgagac tcggttcgac
     7681 ccggcagcca aggagccgct ccaaggattg gggattgagg tgggtcgtcc agtcctgctg
     7741 atcttcaaga ctgaaaagct cgccttttaa gagggggttg tagaggcgga cctctgcgtc
     7801 gacagcatgg gcggccgaaa cccaatggat ggtcgctttg acctttcttt gggcactcgg
     7861 agcgccactc ttcgtttcaa catcataggt gcagtgtagt tctgtgatct ccccggtgcc
     7921 ggcatctttc atgacgccca cacatttgat gatatacgca taacgcaagc ggacttccct
     7981 gcccggagcc agccggaaaa actgtttcgg cggatcttcc aggaaatcgt ccctttcgat
     8041 gtacagggtt cgagagaagg ggagtggtcg tgtgccggcg gagggatctt ccggattgtt
     8101 gatggcctcc agctgctccg tgacgccgtc aggatagttg tcgataatga ccttcaacgg
     8161 cttcaggact gccatgacgc gaggcgcgcg cttattcaga tcctctcgaa tgaagtgttc
     8221 aagcagctgc atttccacta ccgaatcgcg ttttgacacg ccgatgtggt cgcagaacgc
     8281 gcgaatcgct tccggtgtgt agccgcgccg tcggagcccc ttgatggtga gcaagcgtgg
     8341 atcatcccag ccggccacaa gctttttctc gaccagttcg agcaacttcc gtttactcat
     8401 gacggcctgg ctgatattca accgggcaaa ctcaatctgt tgggggcggg cttcggtgtc
     8461 cgctcgttcg acgacccagt cgtagagtgg ccggtgatct tcaaactcca aggtgcaaat
     8521 cgaatgggtc acactttcga acgcatcaga tagcggatga gcgtagtcat acgccggata
     8581 gatgcaccaa gtttttcctg tacggtagtg ggcagcgtgg cggatacgat agaggatggg
     8641 atcccggaga ttcatattgg gcgaggccat gttgattttc gctcgcagga catgtgtccc
     8701 gtctgcgaat tcttctgccc gcatccgacg gaagaggtcg agactctctt ctgcgggacg
     8761 gtcacggtac gggctatttg ttccaggttc ggtcagagtg ccgcggtatc gtctgatgtc
     8821 ctcaggtgag aggctatcga cgtaggccaa acctttccgg atcaggcctt cggcgagcgt
     8881 gtacagccgc tcgaaatagt cagacgcaaa aaacaggttt tctccccagt caaatcccag
     8941 ccagcgaaca tcctcctgga tagcctgcac atattgggga tcttcggtcg tgggattggt
     9001 atcgtcgaat cgtaagtggc acttgccacc gagatgatct tgcgccaaac caaaattcag
     9061 gcagatggat ttggcatggc cgatgtggag atagccgttt ggctcgggtg gaaaacgcgt
     9121 gacgacccag ccgccgtgct tgcctgctgc acggtcggca gacacgatct cgcggatgaa
     9181 attggatgca gtggttccgt ccggcacggt ttcccc
//
LOCUS       DASTQY010000072         8879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_554284, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000072 DASTQY010000000
VERSION     DASTQY010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8879
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554284"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..503
                     /locus_tag="VFR82_02270"
     CDS             51..503
                     /locus_tag="VFR82_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500453.1"
                     /translation="MRHEEACLGMIVRLLSDWHGIPKGTVGSIDTVETGVDGSWCFTI
                     FWEPHCPLRSPMPGRTLAHTGLQSGSLGLRLWEEDLPRFEVISQEQKAVAILHIAPPA
                     DTGPTLLCLFGSQDVDQLTLPFMDQELVTDRELIEDNQPQLTFVSVGA"
     gene            1015..1215
                     /locus_tag="VFR82_02275"
     CDS             1015..1215
                     /locus_tag="VFR82_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500454.1"
                     /translation="MAKSSKPDFLTEERLLACLMFLSKMRKSGATNMFEIRLKLRHAF
                     PDLTTNQAAEVLAYWMQTFAAA"
     gene            3082..3158
                     /locus_tag="VFR82_02280"
     tRNA            3082..3158
                     /locus_tag="VFR82_02280"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3116..3118,aa:Arg,seq:tct)
     gene            complement(4391..4858)
                     /locus_tag="VFR82_02285"
     CDS             complement(4391..4858)
                     /locus_tag="VFR82_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEU4500455.1"
                     /translation="MLTAPFGCLYEVAEQPYVVEVDQKDDSIHKPPPTAGKMLAGKKF
                     KKAKLKGEKFIGAMLAGADLRQADLEDADLRDAMLMGANLSGANLVNANFEGAMLLGA
                     QLEEALIEGANFKNTAFFDQDQIDEACGKPRALPDKLKAPTGVNCKPTTIDPK"
     gene            6303..6968
                     /locus_tag="VFR82_02290"
     CDS             6303..6968
                     /locus_tag="VFR82_02290"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEU4500456.1"
                     /translation="MRARNSIRFYRFYRNAHNPDPVAGIFAHGKFMILAGRFIAILIV
                     MTLAIAMTNLLSQAEESTLISIGPRIGFSQKTPLLGKQQREAFRLYDVAAIFRLPWRW
                     PLNAGKWQLGTGVITSVGLLEAAGDAGVMATAVPDLILTGWDELISFDAGAGAGLFSR
                     HQFGSQNFGGPAQFVATLGIRFSPFTHSYADFHLLHFSDAGLYGSDSLGVDMFIFEVG
                     YKF"
     gene            complement(7361..7885)
                     /locus_tag="VFR82_02295"
     CDS             complement(7361..7885)
                     /locus_tag="VFR82_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500457.1"
                     /translation="MHVTRSMLAFGLLMLAGCQTIGGESMPTLTRTGEVKDVVIRDVV
                     EPTSLTVYPGDEVRWINKRQGTVRVIFLNPMTENLACQRHFGGLMGFGTKRNEYSAKL
                     GPNKTASVCFRDPGEVKYVVRAESSDSSGEHNTAGTISIGPVYETRRPAVSPDERNKG
                     LAARSDKDEAASSR"
     gene            complement(8150..8476)
                     /locus_tag="VFR82_02300"
     CDS             complement(8150..8476)
                     /locus_tag="VFR82_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500458.1"
                     /translation="MRRYLITTALLVGLTPLVAGCTDHYRVTDPMSGKSYYTTKVDSA
                     GRGGAVKIKDEKSGSIVTLQSSEVKTISEEEYEAAMVASAPAQPAPVPPALVRPATPQ
                     GGSTQP"
BASE COUNT         2082 a         2339 c         2299 g         2159 t
ORIGIN      
        1 acgggttggc gatgctatcg taacggcatt tcctactagt ggaggtggtt atgcgacatg
       61 aggaagcctg ccttgggatg atcgtcaggc tcctttcgga ctggcacggc attcccaagg
      121 gcacggtggg aagcatcgac acggtggaga cgggagtaga tggttcatgg tgtttcacga
      181 tattttggga accgcattgc cctttgcgtt caccgatgcc aggccggact ctggcgcaca
      241 ccggcctcca atccggatcc ctcggcttgc gactatggga agaggatctc ccacgcttcg
      301 aagttatcag tcaggagcag aaggctgtgg ccatcttgca catcgcgcca ccggctgaca
      361 cgggtcccac actattgtgc ttatttggct cccaggacgt ggaccaacta acgttaccat
      421 ttatggacca ggaactcgtg acggaccgag aactcattga agataatcaa ccgcaactga
      481 cctttgtctc ggttggagcg tgagatgggg ggcgaagagc gaggatcagg ctgcggcctt
      541 tgatattggc cgtatgtacc tattactcac cactgacaca gcgaagacaa aaacccggag
      601 cccgtttggt ctgaacccca cactcttcca tagcaacttg ttcttgcgct gtaactgccc
      661 tgcacattgc aacgctagca actgctctca ccttcatgag cctctccctt tcggcaagtg
      721 gcctttgcgt tcaagctctg caacatgttc acgcagcagt tggacgacgt atttttgaat
      781 gctgtgacct ccgctcaagg cggcggcagc cttcaatttg ttcaccagat cagcggggac
      841 atttcgtaga tacacaacct taggtttagt agccatgggt cggcgatgat accgcaataa
      901 ctccggccga cacaagctca ctttacctgc tcactgggaa agactatact gcgctagcaa
      961 ctgtcgcagt gctggcattg gcgataaaac gctgtccata aacgggagga tgctatggcg
     1021 aagagcagca aacccgactt tctcacagag gagcggctcc tagcgtgcct gatgttcctg
     1081 agtaagatgc gcaagagcgg tgcaacaaat atgtttgaaa ttcggctgaa gctgcgccat
     1141 gccttccccg atctcacgac aaatcaagct gccgaagtcc ttgcgtactg gatgcagacg
     1201 ttcgccgctg catgaccaat gccatcaccg aagaggaggt tccgtgaaag aagattccgc
     1261 ggccggtcag cgacagcctc aaggccaagc cgaagcagaa acgcctcgaa tttttccgat
     1321 caatggcttc cttcgtcatc cactgcggcg ggaacgggaa aacagcaccg tgccacgctt
     1381 cactgaccaa atggaatacc gctgcttcca tctgcggacc aaaatggtat gggcgatagc
     1441 tgaggaggca gggcagggtg gaggccacct tgggaagggt gtctgcccgc catgccctag
     1501 taaagacgtg catgcggctg caattcgttt aaagaatcga tttacttagg gggcggttca
     1561 gcggcggtgg agccggatgg ggaccgctta aggaattgtg agtcctgccc tcccgtctgc
     1621 gctacaaggt ggctaccggc gtgtcctcaa aagagcggct tatcgatttt cttgcatgtt
     1681 ccttttctgg tcttatatgt gtaccagtac ctgcccttaa cggattcccc ttcgatcttc
     1741 tcactcaaaa ggtactctcc tgtatgccta tttataatga ttgactcgct gactttattg
     1801 cgccgttccc ccgatgcgtt ttgtgtctct ctttgtatct ttagctcggc atcgctaaac
     1861 ttgagttcat actccccatt aaatccctgt actcttttgc tctgccaatc aatcgtataa
     1921 cgcgctttac cgtgatttga tcgtttctct ggcccagaat cactcgtact cgcttcatgc
     1981 gttgtttctt cgtcatattc gcaaccgaaa tggacttctt cggtccatcc tggaactgga
     2041 aaacatattt gtaaggctgc tattagacaa gtgaaaaggc cactggccct tcgattcagt
     2101 gagagatgca tgttaagaca tgttccaaag taagttagct gcatcgcgtt cctcctgctg
     2161 caaaacgtag actctattgc tttgccgacg tcagcctata agcgttcatc acacgcttgc
     2221 gtgaatccat cgaactttac ctttggatgt cgcttgagaa tatcgattta ctcatggaga
     2281 accaaaaaac ctatagacat tcgcttcaac atctattcct tcaaaggctt ccttgagagt
     2341 tcttttcgga atcgacaaca ttaagcacga agtcatcaac tatgacaaaa ctcatatgaa
     2401 aaacaatcat aaggtcactt gtttgatctc gcctctccaa gtctatcact caaatagtat
     2461 ttttggagtt aaggtacaaa tggttgcggg tagataacca gacagcccat gttccgaagc
     2521 gaactcaaga tgaacggcat aggattgccc atggcgagga atgagataga tcacttgatg
     2581 cgtcagcgca ccacctcaaa tggattctgc ttgtacgctt ggaatggcca gatgcagaca
     2641 tgcattggcg tgatttacgt taaggcacac aaagaacggg gaaagccgga gtcccattgc
     2701 cctgacgttt ccctagtgtt tacggagcta aggtaggagc gtagactaga cacacagcga
     2761 gtgtttcggt acaagggact acccattggg gacgttaaaa ccgcctttga taaagagcgc
     2821 aagcgtgctg gaacaaaaga acctgcgcct gcatgagctc aggcatgcgg cgagtacgaa
     2881 ttgaggctgg caggtgtgaa tcctccaaca gccatgaagc ttgttggcaa taagtaagaa
     2941 cgtaagcagc agcgatacaa caccgtggag cctgagaaga tccggagagg aggttccaag
     3001 cttgcgactt atcaggcacc gtaataacac ttgacgcagt ggcgtcaggt gccgaactgt
     3061 aagtgcttgt ctctcctcat cgcgcccgta gctcagttgg atagagcatc agccttctaa
     3121 gctgagggtc gctggttcga ttccagccgg gcgcaccaca caatcaacgg gttgtatttc
     3181 ctatagcagc cgttcgctag cccaccgttg gctttgaggg gatcctacac ctcattgaca
     3241 ttcaaaggag tcgcgctcgc aataccggcc tcgcctgaaa gacgagtgta gtcttgtcca
     3301 atgcaatccc tatccaggga catttcgtct aggttataga gacgggaaag attggtgacc
     3361 tgttctgtac cccaccagaa aacaagatca ccgtcgggtc cgaatgtttt tattgagaag
     3421 gtgtcagact gagcgatttt tagccgaagt ggtggtttcg gggacaatac caacaagcgg
     3481 aacggcggga aatccgaaac cggcgatgat cgaggtacta ggagcagctc cggttcacaa
     3541 acgactgctc gtgaagacta ctggctagag tcgaaggcgc acatgcatcc aatgatattt
     3601 gcatctccat gagcaattga tcgcgatgtg ccttgcaaat gccgtgggaa tggagttccc
     3661 atgccgacga ggaatcatcg agcgtctggt aaagaatggc tggtcttcct tcttgttcac
     3721 accaggcgca taaaatgatc aaggaagccc tccatcaatt cttttggcag tttaagagcg
     3781 tacgagatat ccgcgacctg ctcaacaccc tagatgagag gaaaaaaatc actgcccaaa
     3841 aaacgaccta aacaatgggc gaaaaggtaa gaaaagatac gccctgggtg aaccacgtat
     3901 gagatcgcct taatcttcta agcaacctct gccaaaacgc gagcgaggta gctcaagcgg
     3961 tccatcccac ataactccca aactttgaaa cgctaactcc cggccaggcg tcttggggta
     4021 gggggaagca ggattagcgc caaccgacga cggctcttcc aaccaaacaa gatttgtaca
     4081 attgtgggca gattgggctg gagccgcgga agacttgaga cagttgccct tgttcgccaa
     4141 aaaagaaaag ctaaaacgca tcttacctga cgatcggccc atacccttcg tcgatcatac
     4201 cgtggagcat aaccgtagaa gtggtattga gctgtaatcg gctggcctgc cagctggatc
     4261 ttgaacggat cgtgacaaac gggcgactag gagttatgtc gacagtccga gtgaacgcaa
     4321 ttggaacgag atcaatcccg cgtactagaa ggaagggagg ggaaaccagt tcaagcgagc
     4381 acgctagccg tcatttaggg tctattgtgg ttggcttaca gttcacaccc gtcggtgctt
     4441 tcagtttgtc aggaagagct cttggcttcc cacacgcttc atcaatttga tcttgatcga
     4501 agaaggcagt gtttttgaaa ttggcgccct caatcagtgc ttcctccagt tgagcgccca
     4561 ataacatggc accttcaaag tttgcattca ccaagttagc gccactcaga ttcgccccca
     4621 tcaacatcgc atctcttaag tctgcatcct caagatctgc ctggcgtaag tcagctccgg
     4681 ccaacatggc tcctataaac ttttcaccct tcagcttagc ctttttaaac tttttcccag
     4741 cgagcatttt cccagctgtg ggcggaggtt tgtgtatcga atcgtccttc tgatccacct
     4801 ctacaacgta aggttgttcg gcgacctcat acaagcaacc gaaaggcgcg gtcagcacga
     4861 ttattagtgc acatgaagcc aaatatcgat tacgcattcg tcaacccagg ttttcggtcg
     4921 tagaaggcgt gtgatgggat gccccgaaat atctctttcg tccagggcgc tctcccttca
     4981 cctgttcaca gagccgggca atgtcccgaa gcctcgccgg atccggcggg accagcagtc
     5041 aacccattga acggctcgat acgcgtatcg gacacgtcga gcctacggtt gtagtggctg
     5101 atcatggcct ggatccgcaa gggattagcg ccccacgccc tctcaatgtg ctcatcatct
     5161 ccgtataccg cttaggaggg atttattcga tagtgattct ccaatacgct cccaaaacat
     5221 ggaaacccca aaaagtgatc cgctgaagtg ctgtgagtgt gaaggtgtcc tcttgcctga
     5281 tcgtgcaatt cctctgaagg agacttgggt gctgcgatat gtctgcatag attgtaaagg
     5341 tcggtcatac gcagtgaaga tgggaccagc cgtccgtatg tatccataaa gctcgttgaa
     5401 agtcctttaa agtcgttcat tcaaagtcac tcagttcttt gacggttcta aaacaattaa
     5461 cgttctcatt cgcagcgcga tttgatcatt ctgatttcaa atggtccgat catgagagac
     5521 caagccacac ataatgataa gaagcagatc tccccgagtg cacaaatcgc tcatcataat
     5581 tatctcataa tcgattggcc tcgaaccgct agagacgact cgaggaggcg tattgtagga
     5641 gggtaaataa ctaggcgcaa ggaggcaaag gaaactgctt cgggcgacct tagcgcgcca
     5701 attaggatct aacggctctt aaatctatct gcgtcacact cctaacaatg ggagcgctgc
     5761 ttcgttggcc tacttcttta gtgagtcgtg agttatctcc ctcatcacgg cacataaaat
     5821 caatctcttg catctctaga gcttggtagc tcgatactgc tgattcctgc ggcgtcatgg
     5881 tcacctcggg aaggaaggcg aacttggaga acggattccg tgtaatctcc tggttcgcat
     5941 cacgctttca acttatctgc cgatgcaagc cagagccggt tgacgaggta cagcctccgc
     6001 tttcagctgt acagccttcg cggtattttg gtgaataccg tggtattcat gttgactacg
     6061 aattatgatt taccatgttc tcgatggttc aagcatgtgt taaaggcgcc tatgtcttgt
     6121 ggggtggttg atcctgccat gaagctggtg cgcttctctt ctagaacgag cagggcttga
     6181 caactcgcca cgtggaacga aaacttcttc tcgatcgagc agccgaaggt tgcagtacgc
     6241 cgcggatgtt taatggacct aggctgcatt ttcccataaa ttgtttccca agaaacttct
     6301 tgatgcgtgc acgtaactca atcaggttct acaggtttta taggaatgcc cacaaccccg
     6361 acccggtggc cggaatattt gcccacggga agttcatgat tctcgctgga cgcttcattg
     6421 ctatccttat agtcatgacg ttagccatcg ccatgacaaa tcttctgagc caagcagagg
     6481 agtcgacgct catctctata gggcctcgaa tagggttcag ccaaaagact ccgcttctag
     6541 gaaagcagca aagagaagct tttcgactgt atgacgtcgc tgcgattttc aggcttccgt
     6601 ggcgatggcc actgaacgcc ggaaagtggc aactggggac cggagtcatt actagcgtcg
     6661 gtctcttgga agcagcgggg gatgctggcg taatggcgac cgcggtgcca gacttgatcc
     6721 tgaccggttg ggacgagctc atttccttcg atgctggggc aggcgcagga ctattttcga
     6781 gacatcagtt tgggtcgcaa aacttcggcg gtccagcgca attcgttgcc acactcggca
     6841 tcaggttcag cccgttcacg cacagctatg ccgacttcca cttgctgcat ttttctgacg
     6901 ccggcttata tgggtctgac tctctcggtg tggatatgtt catcttcgaa gtcggctata
     6961 agttttgacg tagtccgtcg aatcggccgg gtaagaatgg ccggcctttg gggggatcaa
     7021 agctttggta cgccgggcga agctgatcca ggttgccgag cgtttccacg tgagaaacct
     7081 gaggctcggg cgtgaagagc cccgtgacgt caacacatcc cgtccgttgt gtttcaggta
     7141 gcgatgggca tgttcccgta gtttttgatc ggtaagccat agagaaagaa ttggcgcatt
     7201 ccgaatgcgc atctctagga ttctatagac gagcgtccaa gaacgagcgg ccgatgtgag
     7261 cagtattctc cctcgctgga tggtgcaagg gcctccgcct tcagagatgg ccgtcaatac
     7321 tccatccaac gagttgagaa cctttgctcc tctcttatca ctaccggctt gaagccgctt
     7381 cgtccttatc agaccgcgct gcaagccctt tattccgctc gtccggactt accgccgggc
     7441 ggcgcgtttc gtatacggga ccaattgaaa tcgtgcccgc cgtattgtgt tctccgctgg
     7501 agtcacttga ttcggctctg acgacatact tcacctcgcc ggggtcccgg aaacacacgc
     7561 tggctgtctt gttcgggccc agtttagccg aatactcatt gcgctttgtg ccgaatccca
     7621 tcagcccgcc gaagtggcgc tggcacgcga gattttccgt catgggattc aagaaaatca
     7681 cccggacagt tccctgacgc ttgttgatcc aacggacctc atcgccggga tagaccgtca
     7741 acgatgtcgg ctcgacaaca tcacggataa cgacatcttt gacttcgccc gtgcgggtca
     7801 acgtcggcat gctttctccc ccgatagttt ggcagccggc cagcatcagt agaccaaatg
     7861 ccagcatgct acgtgtgacg tgcatggtgc tctctccttt tttaatgaag gtgaaacagt
     7921 tctccgatct acacaggtgg tggaaggcca tatgtgggag ggccagcggc ggactacttg
     7981 tccacaccta ggtggaccaa tccgcagcca taaatgagga ttcacgatgg cgaaacttct
     8041 gatggctgat gttgcgtcag gatgcttgcg ttaggactga ccccccgtcg gcgctactcc
     8101 gactccgtcg acttcagctg aactgcgggc aacagggtcg cgcgatctgt caaggctgag
     8161 tggacccgcc ttgtggcgtg gccggccgaa ccaaggctgg cggcacagga gccggctggg
     8221 caggcgcgct cgccaccatc gcagcttcgt attcctcttc cgatattgtc ttcacctcag
     8281 acgactgtaa tgtcacgatg cttcccgatt tctcatcctt gattttcact gccccgcccc
     8341 ggccggcgct gtcgactttc gttgtgtaat aggattttcc ggacatcggg tcggtcactc
     8401 gataatggtc agtgcagccc gcgaccagcg gggtcagtcc gacgagtagc gctgtcgtga
     8461 tcagatatct gcgcatgagt gcctcctcag tttcgttctc accgtatgta tgttgtagag
     8521 tatgcacctc cggtcgagag gcccactgta tagatttgac caccgacgcc ggcggccgtg
     8581 tgctcaattc agcacatgag accgcgcgac agcgaagtct acgacgcctt gttcacgctc
     8641 gggttttact acgatggggc gactcgcatc tcagacggct gcagatcagc agcgctaaaa
     8701 cgtgggtggc gtgtaggggg gcaggaaaga tgaatttgac cgcatcctgc agatctaacg
     8761 tcttgaactt actgacatgc agtgggtgaa ggcggacttc taatctgagg gtcgacggtt
     8821 ccatcccagc cgggcgcacc ctgccatcac acttgtctcc cgtttatcgt tggacttcg
//
LOCUS       DASTQY010000073         3025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_591288, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000073 DASTQY010000000
VERSION     DASTQY010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591288"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..2057
                     /locus_tag="VFR82_02305"
     CDS             312..2057
                     /locus_tag="VFR82_02305"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4500459.1"
                     /translation="MKHDHTHAPEATAVHGFSPGFAALGLEARLLATLEALGYEEPTP
                     IQREAIPPLLAGKDVLGQAATGTGKTAAFTLPLLQRIAHSPKTKPSAIILVPTRELAI
                     QVGEAVGRYGKELRTSVLPLYGGQAIGPQMHALKRGVDVIVATPGRALDHIRRHTLKL
                     EAVQIVVLDEADEMLDMGFAEDLDAILEHTPAAKQTALFSATMPPRIQSIAHRHLQQP
                     VEITIAKEAVKAGSAPRVQQAAYIVTRPYRGATLARIIETAAPKSALVFCRTRVEVDE
                     VTLMLSARGHRVEAIHGGLSQVQRDRVMQAFRTGQTELLVATDVAARGLDIPQVSHVI
                     NYDLPSSAEVYVHRIGRTGRAGREGTAITILDPREQRLLRSIEQHTKGKVVVTPVPSV
                     ADVVAKRLERTRGAVQEAIRAGDLDGFRGVVKAMAEATDLATIGAAAMKLLFRAEGGE
                     RPEQEIPAFPMRQAEWHPSAQRDGHHRDGSSRNVHGRHAVPPGKSRATRGRETGMTRL
                     YVGAGREAGIRAGDLVGAIANEAHINSNLIGAIQIEERFSLVDVPESLAKGIAEVLGR
                     SRIKGRKVGVRLFRE"
     gene            2071..2625
                     /locus_tag="VFR82_02310"
     CDS             2071..2625
                     /locus_tag="VFR82_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500460.1"
                     /translation="MNERTLDGSLAPSRASPRPYNASPPSAVFPMRVLVTSLWLLCLW
                     SGCGIIMVGEPVQKLDISSQDDVEENLKAIRAILADQETRKAPSVAKPSHESSRAAPA
                     SESGPVSEPLLPPTPAPSSSSIARGHLDAPATLPWSPSPSQRPDSPDRPVPAYTVPAP
                     VAPGYSGSIRCVPDGLGGQRCAGH"
     gene            2713..>3025
                     /locus_tag="VFR82_02315"
     CDS             2713..>3025
                     /locus_tag="VFR82_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein"
                     /protein_id="HEU4500461.1"
                     /translation="MASTRIASKKPRPSLDRSIERMRKHLAELAVYVKKGKASRTLEE
                     FDSDSERLIAELLGETSALLDAYQYAQLGEAAGLVNMTEEAPESIGLDSERQSLLQRT
                     RV"
BASE COUNT          621 a          899 c          942 g          563 t
ORIGIN      
        1 ccggggtccc tgcaacataa agtggaaatc accggcatct atggacgtga cgaggcgctt
       61 aaagtgctcc gagccagcca tgccgactac agggaaaagt tccccgaatt ggagtcgctc
      121 tggcccaagc aactgggatg aagagagtac gcgttgcccg ttccctttgt gctcgcgcaa
      181 cgcgcacacc agaatgatct aatattagac tgcggggagg tgctcgttga cgcgcgcacg
      241 tttgagaccg atcgggcaac cctcatgaga agcgtgaaaa agccccctct tgtcgtgaaa
      301 agagattttt gatgaaacat gaccacaccc atgcgccgga ggcgacggcc gttcacggct
      361 tttctcccgg ctttgcggcg ctcgggctcg aagcgaggtt gctcgcaaca ctggaggcct
      421 taggctacga ggagccgacg ccgattcaac gggaagcgat cccaccttta cttgcgggca
      481 aagacgtgtt gggacaggca gccaccggga cgggcaagac cgccgccttt acactgccct
      541 tgctgcaacg cattgctcac agtccaaaaa caaagccgtc ggcgatcatt ctggtgccca
      601 cccgagaact ggcgattcag gtcggcgagg ccgtcgggcg ctatgggaaa gaactccgga
      661 ccagtgtcct gccgctctac ggggggcaag cgattggtcc ccaaatgcat gcgcttaaac
      721 gcggcgtcga tgtgatcgtt gccacaccag gccgcgcgct cgatcatatt cgacgccaca
      781 cgttaaagct cgaagccgtg cagatcgttg tgctggatga agcggacgag atgctcgaca
      841 tgggatttgc ggaagatctc gacgcgatcc ttgagcacac gcctgcggcc aaacagaccg
      901 cgttattttc agccacgatg ccgccgcgaa tccaatcgat cgcgcaccgg cacctgcagc
      961 agccggtaga aattacgatc gcgaaggaag ccgtcaaagc gggttccgca ccgcgggtgc
     1021 agcaggccgc ctacatcgtc acacgaccat atcgaggggc gacgctcgca cgcatcattg
     1081 agacggccgc gcccaagtcg gcgctggtgt tttgccgcac ccgtgtcgaa gtcgacgagg
     1141 tcacgctcat gctgagcgcc cgtggacacc gggtggaagc aatccatggg gggttgagcc
     1201 aggtccagcg ggatcgggtg atgcaggcct tccggactgg gcagaccgaa ctgctcgtgg
     1261 caacggatgt cgccgcccgc ggactcgaca tcccgcaggt ctcacatgtg atcaactatg
     1321 atctgccctc atcagcggaa gtctatgtgc accgcatcgg acggacggga cgggcaggac
     1381 gcgaagggac tgcgattacg attctcgatc cgcgggaaca acggctgttg cgaagcatcg
     1441 aacaacatac gaagggaaaa gtggtcgtga caccggtgcc gtcggtggcc gatgtcgtgg
     1501 ccaaacgatt ggagcgtacg cgaggcgccg ttcaggaggc gatccgggca ggagatctcg
     1561 acggctttcg cggagtcgtc aaggccatgg ccgaggcgac ggatttggcc acgattgggg
     1621 ccgccgcaat gaaattgctg ttccgcgccg agggaggaga gcggcctgag caggagattc
     1681 cagctttccc catgaggcag gcggagtggc atccctcagc acagcgtgac ggtcaccatc
     1741 gggatggctc ttctcggaat gtgcatgggc gacatgccgt gccccccggg aagagccgag
     1801 caactcgagg ccgtgaaacc ggcatgacgc gcctctacgt gggagccggg cgggaagccg
     1861 gcatccgagc cggtgatttg gtcggagcca tcgcgaacga agcgcacatc aattcgaacc
     1921 tcatcggtgc gattcagatc gaagaacgat tttctctcgt cgatgtgcct gaatccctgg
     1981 cgaaggggat tgccgaggtg ctggggcgat cgagaatcaa agggcgcaag gtgggtgtgc
     2041 ggttgtttcg cgagtaacga gatgatgcag atgaacgaga gaacgctgga tggatcgctg
     2101 gcaccctcgc gtgcctcgcc tcgaccttac aatgcttcgc ctccgtcggc tgtgttcccc
     2161 atgcgtgttt tagtcacatc gctgtggctt ctctgcctgt ggtccggctg cgggattatc
     2221 atggtcggcg aaccggttca gaaactggat atttcgtctc aggacgatgt tgaggagaat
     2281 ctcaaggcaa ttcgcgcaat cctggccgat caggaaaccc gcaaagctcc ctctgtggcg
     2341 aagccgtccc atgaatcgtc tcgggcggca cctgcgtctg aatccggacc ggtcagcgaa
     2401 ccgttgcttc ctccgactcc tgctccgtct tcgtcgtcca tcgcgagggg gcatctcgat
     2461 gcgccagcca cacttccttg gtctccgtcg ccttctcaga ggccggattc tccggaccgt
     2521 cctgtgccag cctataccgt gcctgcgccg gtggcgccgg gttattccgg ctccatccgc
     2581 tgtgtgcccg acggcctcgg aggtcagcgg tgtgcgggcc attaggcaaa cgaccgatga
     2641 attcgcggct tgcctccgct gacagggtac aggtaagatt cgcttcctcg cccctaatcg
     2701 actggaggtg ccatggcgtc gactcgcatc gcttccaaga aaccgcgacc gtccctcgac
     2761 cgtagcatcg agcgcatgcg caaacacctt gcggagctgg ccgtctatgt caagaagggc
     2821 aaggccagcc gcacgcttga agaattcgat tcggattcgg aacgcttgat tgccgaactg
     2881 ctcggcgaaa catccgcgct gctcgatgcc taccagtatg cgcagctagg cgaggccgca
     2941 ggactcgtca atatgacgga ggaagcgccg gaaagcatcg gcctcgatag cgagcgccaa
     3001 agtcttctac agcgcactcg cgtcc
//
LOCUS       DASTQY010000074         6179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1763433, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000074 DASTQY010000000
VERSION     DASTQY010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763433"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..647)
                     /locus_tag="VFR82_02320"
     CDS             complement(123..647)
                     /locus_tag="VFR82_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEU4500462.1"
                     /translation="MTKPKPVSKPVAKGKPAAAAVIEKPVMMAVSPLAAKPKETAKER
                     EARERRREALQQMLMGKRQEIMKEIEGSLGQSLTEDQQRRLESARDVGDQALMDLDRE
                     LGISLMEMRNRRRQAIDEALTRLNEGTYGICAECGVEISERRLEAVPFAKLCVECQAR
                     EELLEKIEKEEERD"
     gene            complement(790..1314)
                     /locus_tag="VFR82_02325"
     CDS             complement(790..1314)
                     /locus_tag="VFR82_02325"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU4500463.1"
                     /translation="MTVAEYRALIREVPDFPKPGILFYDITTLLKDGQAFSAIADDLL
                     AYYSGQRVAKVVGIESRGFIYGAILAQRLKAGFVPVRKPGKLPADCFEVKYSLEYGSN
                     SLAIHRDAIGMGERVLIVDDLLATGGTAAATVSLVRQLGGEIVGLDFLVELKELQGRQ
                     KLAGYPVRSTIIYP"
     gene            complement(1319..2275)
                     /locus_tag="VFR82_02330"
     CDS             complement(1319..2275)
                     /locus_tag="VFR82_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4500464.1"
                     /translation="MGRGQDEFSDQSEARRHYSSHEEDDASDNDASSDDGNSRGRSEG
                     LDTLKSYLREIRRSTLLTFKQEQQLGKRVMAGDEQARQQMIESNLRLVISIGKRYIHR
                     GFPFSDIVEEGNLGLIKAVEKFNYKRGFRFSTYASWWIRQYIERAIINQGKLVRLPVH
                     VVERLNRYLNKVEALVQELGREPVAEEIARKMKTTEEEVLDLKQIVRTTCSLDSPIND
                     RADTFLRDVIEDPMCASPADTAEGVMRRAEMMTWVKELPEKEQTVIVSRFGLDGSEAR
                     TLEEIGRDMGLTRERVRQIEMTALSRLRHTIERKTLTQSDLL"
     gene            complement(2300..2596)
                     /locus_tag="VFR82_02335"
     CDS             complement(2300..2596)
                     /locus_tag="VFR82_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU4500465.1"
                     /translation="MTDASDSTAVRARISVSGRVQGVGYRAFTVRNAVQRELVGGVKN
                     CEDGRVELEAEGPKDRIVSLIEVLKAGPPASRVTAVQVEWLPASGRQTNFVVWY"
     gene            2756..2908
                     /locus_tag="VFR82_02340"
     CDS             2756..2908
                     /locus_tag="VFR82_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500466.1"
                     /translation="MTSFRTALTIGRYGRIAGIGDATAAGFMRWSSVAAKDPAPADHG
                     RCVSPD"
     gene            2940..4379
                     /locus_tag="VFR82_02345"
     CDS             2940..4379
                     /locus_tag="VFR82_02345"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEU4500467.1"
                     /translation="MAFSATDLKLVLAEIAPALIDGWIQKIYQPATRVVVFELRAKGA
                     TSRLLVSCEPETARLHLVRHRLRNPPSPPPFCQFLRAHLDGARLQGMRQLREDRIVAM
                     EFTTKSGPRTVVCELIGHAANILVLDEHQHVLRDLNHQRDRVGQHYQPPASQPAALDK
                     KVVARNAAVTRDHCFPVSAAIEADYRQQEARAAEDRTKLEQLRSLRKALKKTRRRIDA
                     WREDLAKATRYRDYARYGELMKANLSSITPAKDQVTLVDYYDESLPEVTIPLDRSKSA
                     IGNMDDYFKKHRKYMAAERELVPRIAQAEDEINALEREIASIEDGTWTPPDKGRSTRL
                     NPVTKRDTSGRARGPFRRFTSGDGLPIFVGRNARENDQLTFGLAKNDDLWLHARGAPG
                     SHVVVRLGKGSDPSPETLRDAATLALLYSDLKKSGKGEIVYTRRKWVKPAKGRALGAV
                     IVTQEKTIHVQLDRTRLEALKARQERSLP"
     gene            4463..4972
                     /locus_tag="VFR82_02350"
     CDS             4463..4972
                     /locus_tag="VFR82_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4500468.1"
                     /translation="MSPSPPSASAGPTVLVVDDDATVVAYCKKILQEAGCSTLSASGS
                     SEALKICKQHGGTIDLLITDLVLPPPEFSFASSENEFPHVHGHELALRALRMRKDLHV
                     ILMSGNPDTDLAGYGIRKDTLPFLSKPFDPSAFVLLVKQTLQTSPPTPELLLKQPSPG
                     TKGADEWFD"
     gene            complement(5057..5392)
                     /locus_tag="VFR82_02355"
     CDS             complement(5057..5392)
                     /locus_tag="VFR82_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU4500469.1"
                     /translation="MGSKIYVGGLPYSATEQQLSDLFGAHGAVASARIITDKFTGQSR
                     GFGFVEMSSDSEAQAAISALNGSDMGGRTLTVNEARPQEPRAGGGGGGRGGFGGGGGR
                     GAGGKRDRW"
     gene            complement(5648..>6179)
                     /locus_tag="VFR82_02360"
     CDS             complement(5648..>6179)
                     /locus_tag="VFR82_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4500470.1"
                     /translation="NLMNLFASPLSPAEFLAATIAMSIFKVTCVSIVMGICAWLFYGY
                     NVLIMGLWLLPFVVNLVITGWVIGVLTTSLIMRFGQEAEILAWSLVFLFQPISCVFYP
                     LDVLPYWLQVVAWMNPAAHIFEGMRDVLREGIVPFAHLAWAGGLNVALLVMTIILFYK
                     TFAYCKVQGSLVRVGE"
BASE COUNT         1360 a         1884 c         1671 g         1264 t
ORIGIN      
        1 gtcggtgttt gggtgccggt cataatgtga gatctcggtt accgtaccga gccgagggct
       61 gacactgagg gaacggacgg acggcggcat gcaatgccaa ccggggccgg gcgaatgcga
      121 aactagtcgc gctcttcttc cttctcaatc ttctcgagaa gttcttctct cgcctgacac
      181 tcgacacaga gcttggcaaa aggcacggcc tccaaccgtc gctcgctgat ctccacccca
      241 cactcggcac agataccgta ggtgccttcg ttcagacggg tgagcgcttc gtcaatggct
      301 tgccgccgtc ggttgcgcat ttccataagc gaaatcccga gctcgcggtc cagatccatc
      361 aaggcctgat ccccgacgtc gcgcgctgac tccagccggc gctgttgatc ttcggtcagg
      421 gattggccca gacttccctc aatctctttc ataatttcct gacgcttgcc catcagcatc
      481 tgctgcaagg cctcacggcg gcgttcgcgc gcttctcgct ccttcgccgt ctctttcggc
      541 ttggcggcca gtggggatac ggccatcatg accggcttct cgattacggc ggccgcggct
      601 ggtttgcctt tcgccaccgg cttgctgact ggtttgggtt tggtcatcgg tttcttcctc
      661 ggcggtggtt tcatcgccat cgcgttgtcc tctcctgggg tgaaacgctg gacggcaaaa
      721 aagttcggca tctataacat gcctactcgg agtttgacaa ggggttgtag gaaaaagggg
      781 cagagccccc tagggataga tgatcgtgga acgcacgggg tacccggcca gcttctgccg
      841 gccctgcagt tctttgagtt cgaccaagaa atcgaggccg acgatctcac cacctaactg
      901 cctgacgagg gagacggtag cagctgcagt cccgccggtg gcgagcagat catccacgat
      961 cagcacccgc tcgcccattc cgatggcatc ccgatggatg gcgagtgaat tggacccgta
     1021 ctcgaggctg tatttgacct caaagcaatc ggccggcaac tttcccggtt tccggacggg
     1081 tacgaagcct gccttgagcc gttgggccag gatcgcccca tagatgaatc cacgcgattc
     1141 gatccctacc actttggcca cgcgctgccc gctatagtag gccaggaggt catcggcaat
     1201 ggccgaaaaa gcctgaccat ctttcaacag cgtcgtgatg tcgtagaaaa gaatgccggg
     1261 tttggggaag tcggggactt ctcggatgag cgctcgatac tcggcaacgg tcattggctc
     1321 agagcaggtc cgattgtgtc agcgtttttc gctcgatggt gtgacgcaag cggctcaagg
     1381 cggtcatctc gatttgacgg actcgctcac gggtcaatcc catgtctcgt ccgatttcct
     1441 caagcgtcct cgcttcgctt ccgtcaagcc caaaccgcga cacaataaca gtttgctctt
     1501 tttcaggcaa ctctttcacc cacgtcatca tctccgcccg ccgcatcacg ccttccgccg
     1561 tatcggctgg cgaggcacac atcggatcct cgatgacatc acgcagaaag gtgtccgccc
     1621 gatcgttgat cggactgtcc agcgagcagg tcgtccggac gatttgtttc agatcgagga
     1681 cctcttcctc ggtcgtcttc atctttcggg cgatttcctc ggcgacaggt tcccgtccca
     1741 gctcctggac cagcgcctcc accttattca ggtaacgatt gagccgctcg accacatgca
     1801 ccggcaaccg tacgagcttt ccctgattga tgatggcacg ctcgatatac tgcctgatcc
     1861 accaggatgc atacgtgctg aaacggaacc cccgcttgta attgaatttc tccaccgctt
     1921 tgatgagccc gaggttgccc tcttcgacga tatccgaaaa cggaaaccct cggtgaatgt
     1981 agcgtttgcc gatactgatc acgagacgga gattcgactc gatcatttgc tggcgtgcct
     2041 gctcatcgcc ggccatcact cgtttgccca gttgctgttc ctgcttaaag gtcaacagcg
     2101 tggaccgccg gatctccctg agataactct tgagggtatc cagcccttcc gagcggcccc
     2161 gtgagttccc gtcgtccgag gaagcgtcat tgtccgacgc atcgtcctcc tcgtggctcg
     2221 agtaatgcct ccgcgcttcg ctctggtccg agaattcgtc ctgtcctctt cccatctcga
     2281 cctcagatcc tcaatgtcgc taataccaca cgacaaagtt cgtctgccgg cctgacgccg
     2341 gcaaccattc cacctggaca gcggtcacgc gggatgcggg cggacctgcc ttgagaactt
     2401 cgatcagcga cacaatgcgg tctttgggac cttcggcttc caattcgacc cggccgtctt
     2461 cacaattttt tacaccgcct acgagttccc gttgcacggc gttgcgaacg gtgaacgctc
     2521 ggtagccgac gccttgtact ctccctgaca ctgagattct ggctcgaacg gcagtcgagt
     2581 cactcgcatc cgtcatcgtt cagcccttgg gatacgcgta acgaagggat agtctcacac
     2641 ctcgagaaac gcgtcacgtg atttgcattt ctacgccaga aaagatctgc ccgatgattg
     2701 tacgtacgca gacatgtatg gagcaacggc gacacgattg gatgctgacg agagcatgac
     2761 atcatttcga acggctctca ccatcggccg ctatggtaga atcgccggca tcggcgacgc
     2821 cacggccgcg gggttcatga ggtggtcctc cgtcgcagcg aaagatccgg cacctgcgga
     2881 tcacggccgc tgcgtatcgc cggattagac tgtgagcagc agatgggagg aaagggcgca
     2941 tggcattcag cgcgaccgat ctcaagctcg tgttagctga gatcgctcca gccctcatcg
     3001 atggttggat tcagaaaatc taccaaccgg caacacgtgt ggtcgtgttc gaactccgtg
     3061 cgaagggagc gacctcccgg cttctcgtgt catgcgaacc agaaaccgcc cggctccatc
     3121 ttgtccgaca tcgcttgagg aacccgccca gtccgccgcc attttgccag tttctgcgcg
     3181 cccatctaga cggggcccga ctccagggga tgcgtcaact gagggaggac agaattgtcg
     3241 ccatggagtt cactacgaag tcaggacccc ggaccgtcgt ttgtgagctc ataggccatg
     3301 cggccaatat cctggtgctg gatgaacatc agcatgtcct gcgcgatctg aaccaccagc
     3361 gagaccgtgt cggtcaacac tatcagcccc ctgcaagcca accagctgcc ttggataaga
     3421 aggtcgtcgc tcgcaacgcc gccgtgactc gggaccactg tttcccggtt tctgcggcca
     3481 ttgaagcgga ttaccggcag caggaagccc gcgccgcaga agatcgaacc aaactagagc
     3541 agctcagatc gcttcgaaaa gcgctcaaga aaacgcgacg acggatagat gcgtggcggg
     3601 aggacctggc aaaagcgacc agataccggg actacgcccg atacggcgag ctcatgaagg
     3661 caaatttgag ttccatcacg cccgcgaagg atcaggtcac cctcgtggat tactacgatg
     3721 aatcgctccc cgaagtcacg atcccgcttg accggtccaa atccgccatc ggcaacatgg
     3781 atgactattt caaaaaacac cgcaaatata tggcggcgga acgtgaattg gtcccacgca
     3841 tcgcgcaggc ggaagatgag atcaacgcac tggagcggga gattgcgtcc atcgaagacg
     3901 ggacctggac gccccctgac aaaggccgat caacccgact gaacccagtg acgaagcggg
     3961 acacatccgg ccgagcacga ggtcccttcc ggcgcttcac gtccggtgat ggtcttccca
     4021 tcttcgtggg acgcaatgca cgggagaatg accaactcac attcgggctg gccaagaacg
     4081 acgacttgtg gctccatgcc cgaggcgcgc ccggatctca tgtggtagtc cggttgggca
     4141 aagggtcgga tccgtcaccg gaaacactgc gggatgcggc gacgctggca ttactctaca
     4201 gcgacctgaa gaagagcgga aaaggcgaga tcgtttacac aaggcgcaaa tgggtcaaac
     4261 ccgccaaggg tcgagccctg ggcgcagtca tcgtcacaca agagaaaacc atccatgtgc
     4321 aattggatcg gacgcggttg gaagccctga aagcccgcca ggagaggtcg ctcccgtaag
     4381 atttcatctt tcgctggccg agcatgtcct cttgagctag gctcagcagg aacgagtgtc
     4441 tcacctaaga gggaggtgtc gtatgtcacc ctcgccacct tcagcaagcg ccggtccgac
     4501 cgtgcttgtc gtcgacgatg atgccacggt agtcgcctac tgcaagaaaa tcctccaaga
     4561 agccggttgt tcaacgcttt cagcgtcggg aagctcggaa gccctaaaga tctgcaaaca
     4621 gcacggaggc accattgatt tgctgattac cgatctcgtg ctacctccgc cggagttctc
     4681 tttcgcctca tcggaaaatg aattccccca cgttcatggg cacgaactcg cactccgggc
     4741 actcaggatg cggaaggact tacacgtgat tctcatgtca ggaaatccgg acactgatct
     4801 cgctggatat ggaattcgga aagacacact gccgttcctc tcgaagccgt ttgacccctc
     4861 cgccttcgtc ttgttggtga agcagacatt gcagacttca ccgcctactc ctgaattgct
     4921 gctcaagcaa ccgtctccag gcacgaaggg tgccgacgag tggtttgact gaccccgcac
     4981 caccctcgtc caaaaatgaa aaggggtgcc atgactcatg acaccccttc gttgtatgcg
     5041 ttacgagcgg tacgacttac cagcggtccc gcttgccgcc tgcaccacgg cctccaccgc
     5101 cgccaaatcc accacggccg ccgccgccac caccagcgcg aggctcttgc ggacgtgctt
     5161 cattcacggt taacgtccgg cctcccatat ccgagccatt gagcgcggaa atcgcggctt
     5221 gtgcctcgga gtccgaggac atctccacga atccaaatcc ccgggattgt cccgtgaact
     5281 tgtccgtaat gatccgcgca gaagccaccg ccccatgcgc gccaaacaga tcactcaatt
     5341 gctgctcggt cgccgaatag ggcaacccac cgacgtaaat cttcgaaccc atgtggggtt
     5401 cctcctttga atgtgacatt gtcttgaaca cgaggaacgg ggaaggagcg ggccgaagac
     5461 gcgagacatg cggcgactta ggtctgactt ccgacgagat tcccggacaa ggcaacatcg
     5521 atgttgcggg ccctacagct gaaccaactc catcgcaagg cccgtacgac gaagtagctt
     5581 agaacctagc atagggggtg gtggattaca acctgagatg tcgtagaaga ttccgaggtt
     5641 gtctggctca ttcgcccacc cgcaccaagg atccttgtac cttacaatag gcaaacgtct
     5701 tatagaacaa gatgattgtc atgactaata gggcgacatt gagtccgccg gcccaagcca
     5761 agtgggcaaa ggggacgatg ccttcacgga ggacatcacg cattccttca aagatatggg
     5821 cagcaggatt catccaggcc accacctgaa gccaatacgg caggacgtca agcggataga
     5881 acacacagga gatcggctgg aatagaaaca ccaggctcca ggccaagatt tccgcctcct
     5941 gcccgaaccg catgatgaga gaagtcgtca acaccccgat gacccatcct gtaatcacca
     6001 aattcactac gaagggaagc aaccacaagc ccatgatcaa cacgttgtag ccataaaaca
     6061 accatgcaca gatgcccatg acgatcgaga cgcacgtcac cttgaagata ctcatcgcaa
     6121 tcgtcgcggc caggaattcg gctggcgaca aaggactggc gaacaggttc ataagattg
//
LOCUS       DASTQY010000075         7779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_351835, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000075 DASTQY010000000
VERSION     DASTQY010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351835"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1596
                     /gene="recJ"
                     /locus_tag="VFR82_02365"
     CDS             <1..1596
                     /gene="recJ"
                     /locus_tag="VFR82_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU4500471.1"
                     /translation="GVTTPDQATTWMSHQLPHDPFLIPDMDQAVERLHRAVVAHEPVC
                     FYGDYDVDGMSATAVYLSFFGSLGANVRAYIPHRKREGYGLNMGAVQRLHDEGISLLV
                     TSDCGTTSHHEVALAATLGMDVIVTDHHQTDEELPPAVAVMNPHRADARYPFLGLCSA
                     GLAYKVAQAYQLRYGEAGVSLESLLDLVALATIADVVPLHDENRGFVREGLSQISRGA
                     RCGIRALKQVAGVVRECTAETIAFKLAPRLNAAGRLDHAMLGVRLLTTESPAEAQQLA
                     DQLEQLNRERQKIEAELMAEAVASLKDQELPAALVLASRRWHVGVVGIAAARLVEKFH
                     RPAIVIAIDEGGVGKGSARTTGGFDLYQGLAACRELLEAFGGHPSAAGVTVRESNLEA
                     LRERFSAVVLEWNRGGMTVPTLQVDAEVQLSEITTRLIKEIGTLHPFGAGNPEPTFAV
                     KGLEVMNSRTVGEKHLKMTVRQGRSMPFDSIGFGMKSLIDRGIPAGTPVDLAFMPEFN
                     HWNGYDRIQLRIRDLRVSDRGTA"
     gene            1596..3773
                     /locus_tag="VFR82_02370"
     CDS             1596..3773
                     /locus_tag="VFR82_02370"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU4500472.1"
                     /translation="MVNQTVRDIDQLLDRVKSYNAEADLNIVRKAYEFSAKVHEGQRR
                     RSGEPYLQHPIAVAGVLTFLRTDVTAIVAALLHDTLEDTVATPEELESEFGKDVVHLV
                     DGVTKIGKITFKSHEEKQAENFRKMLLSMADDIRVVLIKLADRLHNMRTLEHLSQAKR
                     HAIAEETLEIYAPLANRLGIGWIKNELEDLCLKHLKPDVYEVLRTRVAKRDEDRQQYI
                     QEVIELVTKALQEHGLPGQVYGRPKHLYGIYQKMNKQSISFEEVYDLTALRIVTDTKM
                     NCYALVGVIHSLWRPVPGRFKDYIAIPKSNLYQSIHTTVVGPKGEHVEFQIRTEEMHR
                     VAECGIAAHWKYKEQGRVTDRDSKTFGWLHQFVEWHRDLPDNRQFMDSVKLDLFHDVV
                     YVFTPKGIVKELPRGSTPVDFAYAIHTEVGDHCVGAKVNGKIVPLKHLVESGDTIEIL
                     TSPTQTPHKDWLKFVRTSRAKTKIKHWIKIEEQKRSLEIGRRLLESEFRRHGMAPAQM
                     IKSSELAAAARQAGYDTTDELIASVGFGHLPTANVMEKLAASSASLVPEKRATPSTRK
                     AVAGKSDDKGVKVKGARDVLMQLSRCCNPVPGDRILGYITRGRGLTIHAIDCPNLEAL
                     DYDRERLVEVEWDSATPSTHAVKISVMAVDKTGVLANVSSAIAECRANISRAEIATRE
                     DRKAVLDFIVEISGTDHLDQVLKAIERVDGVITARRVRAWQEK"
     gene            complement(3833..4294)
                     /locus_tag="VFR82_02375"
     CDS             complement(3833..4294)
                     /locus_tag="VFR82_02375"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEU4500473.1"
                     /translation="MPAVHTDWVIAAVLALILALPSCTQADDGLIPIQLPGGVIIQAE
                     IASTMKKRAEGLMYREHLAQDRGMLFTFDQAQPWTFWMKNTKIPLDIIWMNEKKQIIH
                     IARNVPICTRTDDGCPQYQPNDPAMYVLELGGGEAERLKLEKGSKLQFRIP"
     gene            4402..4587
                     /locus_tag="VFR82_02380"
     CDS             4402..4587
                     /locus_tag="VFR82_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500474.1"
                     /translation="MKEVRKVHYVKGVFVCPKCRQSYVQEKWIESWRVRCHRCDYRGA
                     LTDVSVEERMEEETFRR"
     gene            4704..5210
                     /locus_tag="VFR82_02385"
     CDS             4704..5210
                     /locus_tag="VFR82_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU4500475.1"
                     /translation="MLVTYHSLSGNTERMAEAVVEGAKSVSGTDVVMKRVSHVTADDL
                     FSSDAVVVGSPVYWSNMSGEVKTFFDNWQFKFGIFPDFKMKNKVGAAFATGGQISSGK
                     EVTMLTILAAMLGNQMIVVSAGGAFGASATTEGESHGIDKKELADAQALGRRVADVAA
                     MLKRRPSE"
     gene            complement(5325..5531)
                     /locus_tag="VFR82_02390"
     CDS             complement(5325..5531)
                     /locus_tag="VFR82_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500476.1"
                     /translation="MYLCLCKGIKDSDVRDAGRAGIVMPCQIKARFGLKDAGCCGRCS
                     KNIQAFVELANSETCLPCKNTVST"
     gene            complement(5679..7472)
                     /locus_tag="VFR82_02395"
     CDS             complement(5679..7472)
                     /locus_tag="VFR82_02395"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEU4500477.1"
                     /translation="MAAMRNGRQTPLSNPPDRWNLGDLVNEPVRKLEQHLASLEADVA
                     RIEAARETLRPDMTSSDFQSIVKVSERLTREVSRLGAYAYLWFSENTKDAKARSFRVQ
                     VEEHLTVLQNRLLFFDLWWQGVDEQNAARLMADAGDVRYHLETIRRYKPYTLSEQEEK
                     VINVKNVTGRSAVNTLYDLVTNGLTFTLHINGRKKRLNREELMTFVRSHKASIRQAAY
                     RELYRVFSAQRDLLAEIYKTLVNDWKSENLSLRRFASPIATRNLGNDVPDGAVEALLS
                     VCAENADLFQQYFRLKASICGIRSMSRYHLYAPHRSETKRYRYADAVRMVLEAYRTFS
                     PKLADLAQQVFTDRHIDAPTRPGKLGGAYCYSVVPGMTPYVMLNYTGDARDIATMAHE
                     LGHAIHGMLAAHHSVFTFHSTLPLAETASVFGERILSDALMQQETNKKVRQGLLLSQL
                     DDIYATVLRQAYFVRFEKTAHEMVAGGATGDQLAQTYLGELRQQFGKAVKVPEEFQWE
                     WLTIPHIFASPFYCYSYSFGNLLVLALYRMYQQEGAAFVPKYLDLLATGGSLSPERIL
                     AAVNVDMTSKMFWQSGFTTIREMIGQLEATL"
BASE COUNT         1681 a         2143 c         2314 g         1641 t
ORIGIN      
        1 ggggtgacga ctcccgatca agcgacgacc tggatgtcgc accagcttcc gcacgacccc
       61 ttcttgattc ccgacatgga ccaggcggtt gaacggctgc atcgggccgt ggtggctcac
      121 gagccggttt gtttttatgg cgattatgac gtggacggga tgtcggcgac ggccgtgtac
      181 ctctccttct tcggatcgct cggcgccaat gtgcgggcct atatccctca tcggaagcgg
      241 gaaggttacg ggttgaatat gggcgcggtg caacggctgc atgacgaggg gatctcgctt
      301 ctcgtgacat cggactgcgg gacgacctca catcatgaag tggcattggc cgcaacgctt
      361 ggcatggatg tgatcgtgac ggaccatcac caaacggacg aggaactgcc tcccgccgtt
      421 gccgtcatga atccccatcg tgcggatgcg cggtatccct ttcttggatt gtgctcagcg
      481 ggactggctt acaaagtcgc gcaggcctat cagctgcggt atggcgaggc cggcgtctcc
      541 ttggaatcgc tgctggattt ggtggcgctg gccaccattg ctgacgtggt gccgttgcat
      601 gacgaaaacc ggggctttgt acgcgagggt ctctcccaga tttcgcgtgg cgctcgttgc
      661 ggcatcaggg cgttgaagca ggtcgccggg gtggtgcgcg agtgtacagc cgagacgatt
      721 gcattcaagt tggccccgag gctcaatgcc gccggcagat tagatcatgc gatgttggga
      781 gttcggctgc tgactaccga gtctccagcc gaggcgcagc agttagccga tcagttggag
      841 cagctcaatc gcgagcggca gaagatagag gccgaactca tggcggaagc cgttgcatcc
      901 ctgaaagacc aggaattgcc ggctgccttg gtcctggctt cccggcggtg gcacgtaggg
      961 gtcgtgggca tcgcggcggc tcggttagtc gagaagtttc atcggccggc gatcgtcatc
     1021 gccatagatg aaggaggtgt cggcaaggga tcggcgcgga ctacgggagg attcgatctg
     1081 tatcaaggac ttgccgcctg ccgggagctc ctcgaggctt tcggcgggca tccgagcgct
     1141 gcgggtgtga ctgtgcgaga atcgaacctc gaagcccttc gggagcggtt ttcggccgtc
     1201 gtgctggagt ggaatcgggg agggatgacc gttccgacat tgcaagtcga tgcggaggta
     1261 caattatccg agattacaac caggctcatt aaagagatcg gtacgttaca tccgttcggc
     1321 gccggtaatc cggagccgac cttcgcggtc aagggacttg aagtgatgaa ttcgcggacg
     1381 gtaggagaga agcatttgaa gatgacggtt cggcaaggac gatcgatgcc tttcgacagc
     1441 atcggcttcg ggatgaaatc cttgatcgat cgcggcatcc ccgctggcac gccggtcgat
     1501 ctggcgttca tgcctgagtt taatcactgg aacggatatg acaggattca gctgcgcatt
     1561 cgcgatctcc gcgtcagcga tcggggaacc gcctgatggt gaatcaaacg gtcagggaca
     1621 ttgatcaact gctggaccgc gtgaagagtt acaacgcgga agcggacttg aacatcgtgc
     1681 gcaaggccta cgagttctcg gccaaagtgc acgaagggca gcggcgtcgc tcgggcgagc
     1741 cgtacctcca acacccgatc gctgtcgcgg gcgtgctgac gtttttgaga acggatgtga
     1801 cggcgatcgt cgccgcattg ctgcacgata ccctcgaaga tacggtcgca accccggagg
     1861 aactggaaag cgagttcggg aaagacgtgg tgcatctcgt cgatggcgtc acgaagatcg
     1921 gcaagatcac cttcaagagc catgaagaaa agcaggcgga aaatttccgc aagatgctgc
     1981 tgtccatggc ggacgacatc cgggtcgtgc tcatcaagct ggccgatcgg ctgcataaca
     2041 tgcgcacgct cgagcatctg agccaagcga agcggcatgc cattgcggaa gagacgctcg
     2101 aaatctacgc gccgctggcc aaccgcctgg gcatcggctg gatcaagaat gagctggaag
     2161 atctctgcct caaacatctc aagccagacg tttatgaagt actgcggacc cgcgtcgcca
     2221 aacgagacga agaccggcag cagtacattc aggaggtcat cgaactggtc accaaggctt
     2281 tgcaggaaca cggactgccc ggacaggtgt acggcaggcc gaaacacctc tacggcatct
     2341 atcagaagat gaataagcag tcgatctcgt tcgaggaagt atacgatttg accgctttgc
     2401 gcatcgtgac tgacaccaaa atgaactgct atgcgctcgt gggcgtcatc cactctctgt
     2461 ggcgcccggt tccaggccga ttcaaagact acattgcgat tccaaaatcc aatctctacc
     2521 aatccatcca taccaccgtg gtcggtccca aaggcgaaca cgtcgaattt cagattagga
     2581 cggaggagat gcatcgggtt gcggagtgcg gcattgccgc gcattggaaa tataaggaac
     2641 agggccgggt cacagaccgc gacagcaaga ccttcggctg gctccatcaa ttcgttgaat
     2701 ggcatcgtga cttgccggac aaccgccagt tcatggattc cgtcaaactg gatctcttcc
     2761 acgatgtcgt ctatgtcttt acgccgaaag ggatcgtgaa ggaactgccg agaggatcga
     2821 cgccggttga ttttgcctat gccattcaca ccgaagtcgg cgaccattgc gtgggagcca
     2881 aggtcaacgg aaagattgtg ccgctgaagc atctggttga aagcggcgac acgatcgaga
     2941 tcctcacgtc tccgacgcaa acgcctcaca aggattggct caaattcgtc cgcacctcgc
     3001 gggccaaaac caaaatcaaa cattggatca agatcgagga acagaaacgc agcctggaga
     3061 tcgggcgccg tctgctggaa tcggagttcc gtcggcatgg catggcgccg gcgcaaatga
     3121 tcaagtcgtc tgaactggcg gccgcggccc gccaagctgg gtacgatact acggacgaat
     3181 tgatcgcgtc ggtcggcttc ggccacctgc cgacggccaa cgtgatggag aagctggccg
     3241 cttcatcggc gagtctagtt ccggaaaaac gtgcgacgcc gagcacgcgc aaggccgttg
     3301 ccggcaagtc cgacgacaaa ggcgtgaaag tgaaaggcgc gcgtgacgtg ttgatgcagc
     3361 tgtcccgctg ctgcaatccc gttcccggcg accggattct tggttacatc acacgcggac
     3421 gtggattgac cattcatgcc atcgactgtc cgaatcttga ggcgctggac tacgatcgcg
     3481 agcgattggt ggaagtggaa tgggatagtg ccacgccaag cacgcacgcc gtcaaaatct
     3541 cggtgatggc cgtggacaag acgggcgtcc tcgccaatgt ctcctccgcc atcgccgaat
     3601 gtcgagccaa tatcagtcgg gctgagatcg ccacccgaga agaccgcaag gcggtcctcg
     3661 actttattgt ggaaatttcc ggcacggatc accttgatca ggttcttaaa gccatcgagc
     3721 gggtggacgg cgtgatcacg gcccggcgtg tcagagcctg gcaggaaaaa taagcgcgtc
     3781 ccttgaaaag agccctcacg caggcgtgcc aagacgcgca gaattcggct ggctacggaa
     3841 tccggaactg gagttttgac cccttctcga gtttgaggcg ctctgcttcg ccaccgccaa
     3901 gttccaagac atacatggcc gggtcgttcg gctggtactg aggacagcca tcgtccgtgc
     3961 gcgtgcagat ggggacattc cgtgcaatgt gaatgatctg ttttttctca ttcatccaaa
     4021 tgatgtcgag cgggatcttt gtatttttca tccagaaggt ccagggctgc gcctgatcga
     4081 aggtaaacag catgccgcgg tcttgtgcta aatgttcgcg gtacatcaat ccttcggcgc
     4141 gtttcttcat cgtgctggcg atctcggcct gaatgatgac tccgcctggg agctgaatgg
     4201 ggatgagtcc gtcatcggct tgggtgcagg aaggaagagc caggatcagc gcaagtacag
     4261 cagcaatgac ccagtcggtg tgcacggccg gcatcctatc gactgcatcg acaccgagtc
     4321 aaggcgacac ggcaggagac gccggcgcct tgcattcccg cctggacctg tgcgattctc
     4381 actcgaaccg tggaggtttg tatgaaggaa gtgaggaaag tgcactatgt gaagggtgtt
     4441 tttgtctgcc ccaaatgccg ccagtcctac gtccaagaaa agtggatcga atcctggcgt
     4501 gtgcggtgcc accgctgcga ctaccggggc gctctgaccg atgtctcggt cgaagaacgc
     4561 atggaagagg aaacgtttcg tcggtgagcc ggcgtgactc catccactcc atctcctgcc
     4621 tgattctttg cctcctatca ttactttcgc cgttcggatg cggcttcgta cgtgcggagg
     4681 agccggctcc ttctgccgtc aaagtgctgg tcacctacca ttcgctgtca ggaaacacgg
     4741 agcgcatggc ggaagctgtc gttgaagggg ccaagagcgt ctccggcacg gacgtcgtca
     4801 tgaaacgagt cagccacgtc acggcagacg atttgttttc ttccgacgcc gttgtcgtgg
     4861 gctcccctgt gtactggtcg aacatgtctg gggaggtcaa aactttcttc gacaactggc
     4921 aattcaagtt cggtatcttt ccggacttta aaatgaaaaa caaggtcggc gccgcctttg
     4981 cgaccggtgg acagatctcg agcggcaagg aagtgacgat gctcacaatc ctcgccgcga
     5041 tgctgggcaa tcaaatgatc gtcgtcagtg ccggtggcgc gttcggcgct tctgcaacga
     5101 cggaaggcga gagtcacggg atcgacaaga aagaattggc cgatgcccag gcgctcggcc
     5161 ggcgggtggc cgacgtggct gccatgttga agcgacggcc ttccgagtag ttcacttctc
     5221 ctcgcgcaac gcgctcatcc atccaaccag agcgcgccca ttgcgcagca actccgcgca
     5281 taatattggg gaaagtgagt gattgggaaa aattgcggag gaaatcaggt gctcacggtg
     5341 ttcttgcagg ggaggcaagt ttcactgttg gcgagttcga cgaaggcttg aatattcttc
     5401 gagcaccggc cgcagcagcc ggcatctttc agaccgaacc tggctttgat ctggcaaggc
     5461 atcacgattc cggcccggcc tgcgtcgcgc acgtccgaat ccttgatccc cttacacaag
     5521 cacagataca tgagcgcctc cctgtccaag aacgattatg agaagcattc tcagtatgtc
     5581 acgtttactg gatcttgtca acaagctcga tcagcggctg taagtactga aattcccagt
     5641 ggttaagtga ggttaagcgg tgagtcgatc cggagacact acagggtcgc ttcgagttgg
     5701 ccaatcatct cgcggatcgt tgtgaacccc gattgccaaa acatctttga cgtcatgtcg
     5761 acgttgaccg ccgcgagaat gcgctccggg gagagggagc cgccggttgc caggagatcg
     5821 aggtacttcg ggacgaaggc tgcaccttcc tgttgataca tgcgatacag ggccaggacc
     5881 aagagattgc cgaaactgta cgagtagcag tagaacggac tcgcaaaaat atggggaatg
     5941 gtcagccact cccattggaa ctcctccggg actttcacgg cctttccaaa ttgctgccgc
     6001 agctcgccta aataggtctg tgcgagttgg tctcctgtcg cacctccggc gaccatctca
     6061 tgggccgtct tttcgaatcg aacgaagtag gcctggcgaa gcaccgtcgc ataaatatcg
     6121 tcgagctggc tcaacagtag gccctggcgg acctttttat tggtttcctg ctgcatcagc
     6181 gcatcggaca aaatgcgctc accgaatacg gacgccgttt cagcgagcgg caacgtcgaa
     6241 tggaaggtga agacggaatg atgcgctgcc agcatcccgt gtatggcatg gccaagctca
     6301 tgcgccatcg tggcgatgtc gcgggcgtcc ccggtatagt tcagcatcac atagggagtc
     6361 atgcctggca ccacgctata acaatacgcc ccccccagct tgcctggtct cgttggagca
     6421 tcgatgtggc gatcggtaaa aacctgttga gcgagatcgg ccagcttggg tgaaaatgtg
     6481 cgataggcct ccagcaccat ccgcacggcg tctgcatatc gatagcgctt ggtctcgctc
     6541 cggtgaggtg catagaggtg atatcggctc atggagcgga tgccgcaaat gctcgccttc
     6601 agtctgaagt actgttgaaa gagatcggca ttttcagcac agacggacag caaagcctcc
     6661 acagccccgt ccggcacgtc gttgcccagg ttgcgtgtcg caatgggtga cgcgaaacgg
     6721 cgcaggctca gattctcgga tttccagtcg ttgaccagcg tcttgtaaat ctctgcaagc
     6781 aagtctcgtt gagcggagaa gacgcggtac agttcccgat acgcggcttg gcgtatcgat
     6841 gccttgtgac tgcgtacgaa ggtcatgagt tcttcgcggt tgaggcgctt tttcctgccg
     6901 ttgatgtgca gagtgaaggt caacccattc gtgacgagat cgtatagggt gttgaccgca
     6961 ctgcgaccgg tcacgttttt cacgttgatg actttttctt cctgttccga caacgtatac
     7021 ggtttataac ggcggatggt ctccagatgg taccggacat cgcccgcgtc ggccatcaat
     7081 cgtgccgcgt tctgctcatc gactccctgc caccagagat caaaaaacag gaggcggttc
     7141 tgcaacaccg tcaagtgctc ctcgacttgc accctgaacg agcgcgcctt ggcgtccttc
     7201 gtgttttccg agaaccacag ataggcatag gcgccgagcc gagaaacttc tctggtcaga
     7261 cgctcgctga ctttgacgat cgattgaaaa tcactactcg tcatatcggg tcgaagcgtc
     7321 tcccgggccg cttcaatccg cgccacgtct gcctcgagac ttgcaagatg ttgctcaagt
     7381 ttccgaaccg gctcgttcac caagtcgcct aaattccagc gatccggcgg attgctcaaa
     7441 ggtgtctgtc tgccgttcct cattgcggcc atgcgctgct ccttctcaga ggctcctggt
     7501 cctgaggatc ttaccatccg cacgacactt cgagcagaaa aaatcccacg tcgaccccgc
     7561 cttgtgcgtt gacacctatt ccagcaggat gtgaaaatct ttgcaggtca tacgcatgaa
     7621 cgatcacgaa atcaaccgcg ccgttcaata tgtcacggcg agcacctcct acgggcggga
     7681 gaccgtcgcc acgatcctca agacgggttt tggagaattg acggatttgg ctgttcaatc
     7741 caagcggctg ttcgagcgcg acgccttgct ggaatacgt
//
LOCUS       DASTQY010000076         3682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_151817, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000076 DASTQY010000000
VERSION     DASTQY010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151817"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            391..1839
                     /locus_tag="VFR82_02400"
     CDS             391..1839
                     /locus_tag="VFR82_02400"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU4500478.1"
                     /translation="MQNRPLVPYVVTVVAGAGVLLASAALHFLWPDWRWHHEPLHSAI
                     EAVGGLIVIATATVLLQARNEVTADRYRMLAAGFLGMGILEEFHAVAQPGNGFVLFRN
                     LASLLGGIGFAMAWRPPARTDSSDQRRHAWMMATGALLVGSWFVVFPNHIPEMVRNGE
                     FTPLAVAPQTVACILFLVTGARLLSEYRRSGRTDDALFASLALLYSLAEFVFIYSIPW
                     DNRWWFWHALRLMACLLALGYIGRKYFQVTSDLQTALEQTLRAKETLGQNETRLRQMF
                     DDRERMAQDLHDSTIQSLFALGLGLERCQRLVATSYQDVATQLEQAVKSLQTVIRDLR
                     GYILGLESPISNGRVLEAALSSLVNDMNNAPQRHFRLEVNSAAVDRLTADQLQHLLPI
                     AREAMSNSLRHSGAHAGALSLQLHEGWVRLIVEDDGVGFDAATLQRHGHGLKNLETRV
                     KKLGGHLEVVSGVGQGTRIVCDFPREQDDVKI"
     gene            1823..2488
                     /locus_tag="VFR82_02405"
     CDS             1823..2488
                     /locus_tag="VFR82_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4500479.1"
                     /translation="MTSKSETKPIKVLLVDDHEVLRIGLRTLFTEAGNFQVVGEAGTI
                     AAAVAEALRLEPNVVLMDVRLPDGSGIDACRTIRTAHPDTRVLFLTSFADDDAVLATI
                     LAGADGFLLKEVTSEQLIEAVKAVSQGRSILDPAVTQRVLTKVRSLATLASQDKREPL
                     SPQEERVLALVAEGKTNKEIAVSLNLSDKTVGHYLENIFQKLQVTRRAQAAVYFTQQH
                     NKP"
     gene            2627..3094
                     /locus_tag="VFR82_02410"
     CDS             2627..3094
                     /locus_tag="VFR82_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEU4500480.1"
                     /translation="MLRLTSHMRVLCFICATAVPFMFALSPDQAMAAAADKVKVLYHV
                     DGKDPEVAKYALALINKHIDAEGGPDKIDVELVVHGPALELFEKDRMDAEMTKRFDQI
                     IEKGVKAEMCQVSMKAFGKTIENLAKGFVATLHPVAVKRIADLQQQGYLYIKP"
     gene            3292..3609
                     /locus_tag="VFR82_02415"
                     /pseudo
     CDS             3292..3609
                     /locus_tag="VFR82_02415"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
BASE COUNT          822 a          980 c         1050 g          830 t
ORIGIN      
        1 tttgttgaag tgaaaacttc ttcttctaga ttgttgagat tataacgcga ctgtcaggag
       61 ccaagatgta tagaattgac cggtgggtag gcgagtcgga tgttctcagc gcaagtcctg
      121 aattgattat gggcgatcgg acacgaatag tggggcaaaa tatagcaggt agcgggtgcc
      181 ctccaccaaa aaataattca cctaagttaa atatctagtc acttctgctc ttctgtaatt
      241 tctgtattga agagccctcc tctcccacgc tcatgtctct gccgttggtc attgaacact
      301 gctagcgagt cttcaagtcg caagatgctt attcaagact tgatccgtcg aacttgatct
      361 tcacgctgct tatctgccat cgtgcgcacc atgcagaata gaccgctcgt accgtatgtt
      421 gtcacggtcg tggcgggcgc cggagtgctg cttgcgagcg ccgcattgca cttcctgtgg
      481 cctgattggc gttggcacca tgagccgctc cattccgcca tcgaagccgt aggaggtctg
      541 atcgtcatcg cgacggccac cgtcttattg caggcacgca atgaagtcac cgcggacagg
      601 tatcgaatgc tggctgcagg gttcctcgga atgggcattc tcgaagagtt tcatgccgtt
      661 gcccagccgg gcaatggctt cgtcctattt cgaaacttgg ccagcctctt gggcgggatc
      721 gggttcgcga tggcctggag accgcccgct cggaccgatt catctgacca gcgccggcat
      781 gcgtggatga tggcgactgg tgccttgctg gttggctcgt ggtttgtggt ttttccgaat
      841 cacatccccg aaatggtgcg aaacggcgaa tttaccccgc tcgcagtcgc cccacaaacc
      901 gtagcctgta tcttgtttct ggtcaccggg gcacgtttgt tgtcggagta tcggcgctcg
      961 ggccgaacag acgatgcact gttcgcgagc ctggccctcc tttatagcct ggcagaattt
     1021 gtgtttatct attcgattcc ttgggacaac cgctggtggt tctggcatgc attgcgcctc
     1081 atggcctgtc tgctagcgtt gggatacatc ggtcgaaagt attttcaggt cacttccgac
     1141 ttgcaaacgg ctttggaaca aacgctcagg gcaaaagaga ccctaggtca aaatgagaca
     1201 cggctccgtc agatgttcga cgaccgcgag cgcatggcgc aggaccttca cgacagcacc
     1261 atccagtccc ttttcgcgct cggactcggt cttgaacgat gccagcgtct cgtggccaca
     1321 agctaccagg atgtcgccac tcagttggag caagccgtaa agagccttca aactgttatc
     1381 cgggacctcc gcggatacat actcggtttg gagtcaccga tctccaacgg gcgcgtgctc
     1441 gaagctgcgc tctcctctct agtcaacgac atgaacaatg caccccagcg ccattttcga
     1501 ttagaagtga attctgcagc cgtagaccgc ctgaccgctg accagttgca acaccttctg
     1561 cccattgctc gggaggccat gagtaatagt ctgcgtcact ccggcgcaca tgcgggtgcc
     1621 ctctcgcttc aactgcacga aggatgggtt cgtctgatcg tggaggatga tggagtggga
     1681 ttcgatgctg caacactcca gcggcatggt catggactga agaatctgga gacccgcgtc
     1741 aaaaagttgg gcggtcacct ggaagtggta tccggtgtcg gtcaagggac acgaattgtg
     1801 tgcgactttc ctcgggagca ggatgacgtc aaaatctgaa acgaaaccca tcaaggtctt
     1861 gttggtggac gatcacgaag tccttcgtat tgggctgcgg acattgttca cggaagcggg
     1921 aaatttccag gtcgtcggtg aggccggcac catcgccgcg gcagtggcag aggcattgag
     1981 actggagccg aacgtcgtct tgatggacgt cagacttccg gacggaagcg gaatcgacgc
     2041 gtgccgcacg attcgaaccg ctcatcccga cacgcgggtg ctgtttttga cgtcgtttgc
     2101 agacgatgac gccgtgctcg ccaccatcct ggccggagcc gatggctttc ttctgaaaga
     2161 agtgacgagt gagcagctga tcgaagccgt caaagccgtg tcgcaaggac ggtcaatcct
     2221 ggacccggcc gtcacccagc gtgtgctcac caaagtgcgg tcgctcgcga cgctggcctc
     2281 acaggacaaa cgggagccgt tgtcgccgca agaagaacgc gttttggcat tggtcgccga
     2341 aggcaaaacc aataaagaaa tcgcggtatc gttgaacttg agcgacaaga ccgtcgggca
     2401 ttacctcgaa aacatcttcc agaaactgca agtcacccgc cgcgcgcaag cggccgtcta
     2461 tttcacccaa cagcacaaca agccataggg gtatttgccc ctgtgtcata ggggaagaat
     2521 agtttcctag gcggagcgac cagccctacc gcgctctgtt catggtcagt acgatgggga
     2581 catgaagatg gttcctacat cacgttttca taacaaggag ggtttcatgc tgagactcac
     2641 gagccatatg cgggtgctgt gcttcatctg cgcgaccgcg gtcccattta tgttcgctct
     2701 gagcccggat caagcgatgg cggctgcagc agacaaggta aaggtgctct atcacgtcga
     2761 tggtaaagat ccggaggtgg cgaaatacgc cctggctctc atcaataagc acatcgacgc
     2821 cgaaggcggg cccgacaaaa tcgatgtcga actggtagtc catgggcccg cacttgagtt
     2881 gttcgagaaa gatagaatgg acgcggagat gacgaagcgg ttcgaccaaa ttatcgagaa
     2941 gggtgtaaag gccgagatgt gtcaggtatc gatgaaggcg ttcggcaaga ctatcgaaaa
     3001 tctggcaaag gggttcgtcg ccacgctcca tcccgtggcc gtgaagcgga ttgctgattt
     3061 acagcagcaa ggctatctct acatcaagcc atgacgtgga gggaattgtg cgacagatca
     3121 tcctgggaat cgccctttgc ttaatcgcgc tgtgtggccc gacggcattg cgtgcaacgg
     3181 cagctggcgc accggctccg gctttcgaat tggtcacttt gaccggcgag tcctacagcc
     3241 gagattcgct gaaaggccga ccgacgctgc tgatcttctg gggcaccctg gtgcaatgtt
     3301 tgtcagcgcg agctgccgca gctgtccgaa ttttatcaac gaagcaagcc ggggaagctc
     3361 ggtgtggtct cgatcggatt cgcggataca agaacgaacg ttgaacaatt cgtcaaagag
     3421 cggcccaaaa cgttcgtcta tccagctgca tacgacgaag accgttgggt ggcgctggcc
     3481 ttcaaggtta gcgccactcc aacatacgtg ttattggatg ctcaagtaag catcgtactg
     3541 gtccatcgag gggaaggcgt cttgcagaac cctcaattac aggaattgtt atcaagtatg
     3601 gaccgttaag gcagacgtgt tcgattgatt gcagtggaga tgttgaaatt cgcaaaccgc
     3661 ctgacaagag caaccccttg aa
//
LOCUS       DASTQY010000077         9405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1156580, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000077 DASTQY010000000
VERSION     DASTQY010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9405
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156580"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            474..1631
                     /locus_tag="VFR82_02420"
     CDS             474..1631
                     /locus_tag="VFR82_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU4500481.1"
                     /translation="MWSIALGATIALLLTLAGCNGEPASGGGQPPPPEVAVAQVLTKS
                     VQQWDEYTGRVSAIDTVELRPRVSGYVQRVAYGEGEDVKQGDLMFQIDPRPYRAALDN
                     AQAQLARARVAEQLEAVRIKRAQSLIDDDAISREEFDLRRAAHEQSMADIRAAEAAVA
                     TAKLNLSFTEVRAPVAGRASRALVTVGNLATADQTLLTTLVSQDPMYVYFDADENSYL
                     RYKEQERKSERTGRDNTVYVGLANEDGYPHVGTVNFLDNQVNPTIGTVRARAVVSNAD
                     RMFTPGLYARVRFASGQKADALLIDDKAVLTDQDRKYVYVVDKDGKAQRKDVVLGRMV
                     EGLRVVQSGLAPDDKVIVAGLQKIFYPGIPVKANEVPMGTLRAAPAAVPEK"
     gene            1641..4838
                     /locus_tag="VFR82_02425"
     CDS             1641..4838
                     /locus_tag="VFR82_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEU4500482.1"
                     /translation="MDFSKFFIDRPIFAAVLSIVIFVGGLIAIPILPISEYPEVVPPA
                     VIVRATYPGANPKVIAETVSTPLEEQINGVEHMMYMKSVAGSDGVLQMTITFRPGTDP
                     DAAQVQVQNRVSQALSRLPSEVVRLGVTTQKESPNFLVLVQLLSPNGTYDALYLRNYA
                     NIKIKDELARLPGVGQVQIFGSGDYAMRIWLDPDKIASRGMTAGDIIRAVQEQNIQVS
                     AGQLGAEPIAKGSDFLISINAQGRLRTAEEFGQVVLKAGANGEIVHLADVASIELGAN
                     DYTLRGQLDNRDAPPIGIFQAPGANALTVRDAVIAKMEELKTRFPPDLIYRSDYDTTV
                     FVRDSITAVVQTLLEAILLVVLVVILFLQTWRASVIPLIAVPVSVIGTFAALYLLGFS
                     INTLTLFGLVLAIGIVVDDAIVVVENVERNIEEGRTPLEAAHQAMKEVSGPIVAIALV
                     LCAVFVPMAFLTGVTGQFYKQFAVTIAISTVISAINSLTMSPALAAKLLKSHGTPKDG
                     LSRWIERLFGWVFRPFNRFFKASSDKYQGTVSRALKHRGAVFAVYALLLLGTGLIFQA
                     VPRGFIPTQDKLFLMAGVKLPEGASLERTDTVLHRVVEISKSVDGIAHVVAMTGLNPL
                     QFTNTPNYGIAFLILKPFGERHRSAKEISEEMNGKLAGIQEGMTFVLMPPPILGLGNG
                     AGYSLFIEDRVGLGFGALQNAVNALQGAVMQTSGMGFPISGYQSNVPQLDVEVDRVKT
                     KAQGIALTELFNTLQVYLGSAYVNDFNAFGRTWRIYAQADGDFRGKVEDIANLKTRND
                     RGEMVPIGSLVKLRPTYGPDPVLRYNGYPAADFLGEADPTKLSSAQAMDNLRQIAAKV
                     LPNGMTIEWTDLSFQEASQGRAALLVFPVAILLAFLVLAALYESWTLPLAVILIVPIC
                     MLCALAGVKLTGGDNNTFVQVGLVVLMGLACKNAILIVEFARELELQGNGTIQAALEA
                     CRLRLRPIVMTSVAFIAGTVPLVLSHGAGAEVRSATGITVFAGMIGVTLFGLFLTPVF
                     YVGLRLLSGRKLISHDQTMPRGALENAYSQVEAAR"
     gene            5107..5484
                     /locus_tag="VFR82_02430"
     CDS             5107..5484
                     /locus_tag="VFR82_02430"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU4500483.1"
                     /translation="MEFMILMIPSVYRNNKKLGPNFVPDPKVIEEMTKFNQELSKSVK
                     LLSLNGLHPLTNGARLSFSNGKATVTDGPFIETKEVLGGYWLVEAKSKDELVKLMQNC
                     PAEEGDIIEIRQIFGDEDFAIKK"
     gene            complement(5996..6853)
                     /locus_tag="VFR82_02435"
     CDS             complement(5996..6853)
                     /locus_tag="VFR82_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4500484.1"
                     /translation="MKTVLRSGTAGDATACGSICYEAFKGIAEQHNFPPDFPSPDIAI
                     SLFSMVLSRADVYSVIAEIDDRVVGSNFLWEGDTIAGVGPITVVPAAQNCSVGRQLME
                     RVLERAHARQHVGVRLVQTAYHNRSLSVYTKLGFHAREPLSVLQGPPLNFELPGYRVR
                     SATLDDLGECNELCLNVHGHDRRGELAYAIADGTARIVEYGGRITGYATNIGFFAHAV
                     GRTNEDLKALIGTAPTFVGPGFLLPTRNAELLRWCLTQGLRVVQPMTLMSRGLYQNPT
                     GAFLPSVLY"
     gene            7105..7482
                     /locus_tag="VFR82_02440"
                     /pseudo
     CDS             7105..7482
                     /locus_tag="VFR82_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(7411..7560)
                     /locus_tag="VFR82_02445"
     CDS             complement(7411..7560)
                     /locus_tag="VFR82_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500485.1"
                     /translation="MARLIHGDLDGAMARLLFHVHGTLAVLEQERGQRMAEIMEAYLA
                     HTGTL"
     gene            complement(7725..7801)
                     /locus_tag="VFR82_02450"
     tRNA            complement(7725..7801)
                     /locus_tag="VFR82_02450"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7765..7767),aa:His,seq:gtg)
     gene            complement(7879..8589)
                     /gene="pyrF"
                     /locus_tag="VFR82_02455"
     CDS             complement(7879..8589)
                     /gene="pyrF"
                     /locus_tag="VFR82_02455"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU4500486.1"
                     /translation="MKVVARDRLILALDLPSAVDAERLMDQVQDQVTFVKVGLELYTA
                     AGPEIVQRLLKRGKRVFLDLKFLDIEETVRRATERVAAMGVEFLTVHANRKALAAAVK
                     GRGDSALKLLAVTVLTNFDSHDLREMGIQHTVQELVTARALLAAEMGCDGVVASGEEP
                     GVLRPKVGPRFVIVTPGVRPAGEDVDDHARSTTPTQTIAAGADYLVIGRPIRAAADPA
                     AAAAAVLSEMQAAFDARS"
     gene            8687..8962
                     /gene="rpsT"
                     /locus_tag="VFR82_02460"
     CDS             8687..8962
                     /gene="rpsT"
                     /locus_tag="VFR82_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU4500487.1"
                     /translation="MPVIHKSTIRRARQAEKRQARNRAALSAVKSLVKKVHAAVTDKK
                     ADEAKASLQIATSALSKAVSKGVLKPNTASRRISRLTRHVNSLVASR"
     gene            complement(8937..>9405)
                     /locus_tag="VFR82_02465"
     CDS             complement(8937..>9405)
                     /locus_tag="VFR82_02465"
                     /inference="COORDINATES: protein motif:HMM:NF044544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500488.1"
                     /translation="PGASVFDLAAAIGAQQRGTALAILARNLEAGEAPLRILGSLVWQ
                     YRRLWKVKELVKQAGREGEAARLLRMDPYKIRPFLGQFSDTHLREAFRLFFETDQKLK
                     GGSGGKPAMLLDQLMFKLCNRPQPTRSPSPAAPAPRGRTLSNVRTVSVRRPKN"
BASE COUNT         2037 a         2808 c         2719 g         1841 t
ORIGIN      
        1 taagccggca gaagtttaaa ccaaagctac tcgttccggc gactcttcat cgctttaaag
       61 aaaaccgaag gggtaaccaa gcagcgcatc tgggttcaat atgtaaaagt cactgagggg
      121 agccaggcag tcatcaggaa cgtacaaaat gcaaaagatg aatgacatgc catttgtgta
      181 aggcggacgt tgccataact agcctaccca gcaggtcctc taatgcgcca gccctacgct
      241 cttgaacgac ggtctcgtta aagccattat cagcaggata agtgacctta ttgtctcagc
      301 ggtgcggcat gctggttgtt gcttgtcgat tttggcatac tgaaccgact atatggtaaa
      361 ggcagccaat ccatagcaag gagaacgtaa ggatacggtc gagtgggttc cggatatccg
      421 cctccctgtt ccccggcatc atgcattacg atcaagcata caacgtctct cgtgtgtggt
      481 cgattgcgct cggtgcgacc attgcgttgc tgctcacact ggcaggctgc aacggcgagc
      541 cggccagtgg tggtggccag ccgccaccgc ccgaagtcgc cgtcgcgcag gtgcttacca
      601 agtccgtgca gcaatgggac gaatacaccg gccgcgtgag cgcgattgac actgtcgaat
      661 tacgtccccg cgtcagcggc tacgtacagc gtgtcgccta cggagaaggt gaggacgtga
      721 agcaaggcga cctgatgttc cagatcgacc cccgtcccta ccgtgcggca ctggacaacg
      781 cccaggcgca actggcccgc gcgcgcgtgg cggagcaact cgaagcggta cggatcaaac
      841 gcgctcagtc gttgatcgac gacgacgcga tttcacgcga ggagttcgat ctgcgccgcg
      901 ccgcgcatga gcagagtatg gccgatatcc gcgccgccga ggcagcggtg gctacggcca
      961 agctcaacct gtctttcacc gaggtgcgcg cgccggtcgc ggggcgggcg agccgggccc
     1021 tggtcacggt cggaaatctg gccacggcgg accagactct actgacgacg ctcgtgtcac
     1081 aggatccgat gtacgtctat tttgatgccg atgagaacag ctacctgcga tacaaggaac
     1141 aagagcgcaa gagcgagcgc acagggcgtg ataatacggt gtacgtcggt ctggccaacg
     1201 aggatggcta tccgcatgtg ggcacggtca acttccttga caatcaggtc aacccgacca
     1261 tcgggaccgt acgcgcacgg gctgtggtct cgaatgccga tcgcatgttc acccctggcc
     1321 tgtacgcacg cgtgcgtttt gccagcggcc agaaggccga tgctctgctg atcgacgaca
     1381 aggccgttct gaccgaccag gaccgcaagt acgtttacgt ggtggacaag gacggcaagg
     1441 cgcagcgcaa ggatgtcgtg ctcggccgca tggtcgaggg gctgcgcgtg gtccagtcag
     1501 gcctcgcgcc ggatgacaag gtcatcgtgg ccggcctgca aaagatcttc tatcccggca
     1561 tacccgtcaa ggcaaacgag gtgccgatgg gcacgctccg agcagcgcct gcagccgtac
     1621 cggagaagtg agcgacagac gtggactttt ccaagttctt catcgatcgg ccgatcttcg
     1681 ccgcagtgct gtccatcgtg atcttcgtcg ggggcctgat cgcgatccct atccttccca
     1741 tcagtgaata tccggaggtg gtgccgcctg cggtgatcgt acgcgcaacc taccccggcg
     1801 ccaaccctaa ggtgatcgcc gaaaccgtgt cgacgccgtt agaagagcag atcaacggcg
     1861 tcgagcacat gatgtacatg aaatcggtgg cgggatcgga cggtgtcttg cagatgacga
     1921 tcacgttccg tcccggcacc gatcccgatg ccgcgcaggt ccaggtgcag aaccgcgtca
     1981 gccaagcgct gtcgcggctg ccgtccgagg tcgtccgtct cggcgtgacc acacagaagg
     2041 aatcgcccaa cttcctcgtg ctggtccagc tgctctcacc gaacggcacg tatgacgcgc
     2101 tctacctgcg caactacgcc aacatcaaaa tcaaggatga actggctcgg ttacccggtg
     2161 tcggtcaggt gcagattttc ggcagcggcg actatgcgat gcgcatctgg ctcgacccgg
     2221 acaagattgc gtcgcggggg atgacggccg gcgacatcat ccgcgccgtg caggaacaga
     2281 atatccaagt ctcggccgga cagctcggcg cagaacccat cgccaaaggc agcgactttc
     2341 tgatctcgat caatgcccaa ggtcgcctgc gcacggccga ggagttcggc caggtcgtat
     2401 taaaggcggg cgcgaacggc gagatcgtgc acctggccga tgtggcgagc atcgaactcg
     2461 gcgccaacga ctacacgttg cgcgggcaac tcgacaaccg agacgctccg ccgatcggca
     2521 tcttccaagc accaggggcc aacgcgctta ccgtgcgcga tgccgtgatc gcgaagatgg
     2581 aagaactgaa gacgcgtttc ccgcccgacc tcatatatcg ctcggactac gacaccaccg
     2641 tcttcgtacg cgattcgatc accgctgtcg tgcagacact gctggaagcg attctgctcg
     2701 ttgtgctcgt cgtgatcctg ttcctgcaaa cctggcgcgc gtcggtcatt ccgttgattg
     2761 ccgtgcccgt gtcggtgatc ggaaccttcg ccgccctgta tctgctcggt ttttcaatca
     2821 acacactgac tctgttcggc ctggtcctcg cgatcggcat cgtcgttgac gacgccatcg
     2881 tggtcgtcga gaacgtcgag cgcaacatcg aggaaggccg aacaccgctc gaggccgcgc
     2941 atcaggcgat gaaggaagtc tcaggcccca tcgtcgcgat tgcactcgtg ctgtgcgccg
     3001 tgttcgtgcc gatggcgttt ctgaccggcg tcaccggcca gttctacaaa cagttcgcgg
     3061 tgacgatcgc gatctccact gtcatctcgg cgatcaattc actgaccatg tcgccggcgc
     3121 tggcggccaa gttgctcaag agccacggca ctccgaagga tggtctttct cgctggatcg
     3181 agcgtttgtt cgggtgggtg ttccgtccct tcaaccgctt tttcaaagca agttccgaca
     3241 agtaccaagg cactgtctcg cgcgccttga agcatcgtgg cgcggtgttt gccgtctacg
     3301 cgctgctgtt gctcggaacc gggctgatct tccaagcggt gccgcggggc tttatcccga
     3361 ctcaggataa gctgttcctc atggccggcg taaaattgcc ggaaggagcc tctctcgaac
     3421 gcacggatac ggtgttgcac agggtggtcg agatcagcaa gagcgtggat ggcatcgccc
     3481 acgtcgtggc aatgaccggt ctgaacccgc tccaatttac gaacacgccg aattacggca
     3541 tcgcgtttct catcttgaag ccgtttggcg aacgtcaccg cagcgccaaa gagatcagtg
     3601 aagagatgaa tggaaagctc gcgggcatcc aggaaggtat gaccttcgtc ctcatgccgc
     3661 cgccgattct cggcctgggc aacggtgccg ggtattccct gttcatcgaa gatcgcgtcg
     3721 gcctcggatt tggcgcgctg caaaatgccg tcaatgccct ccagggggcg gtgatgcaga
     3781 cttctggaat gggatttccg atcagcggct atcagtccaa cgtgccgcag cttgacgtcg
     3841 aggtggatcg cgtgaagacc aaggcgcagg gcattgcgct gaccgagttg ttcaacacgc
     3901 tgcaggttta cctcggttca gcctacgtga acgactttaa cgcgttcggc cgcacctggc
     3961 gcatctatgc gcaagccgac ggagattttc gcgggaaggt cgaggacatc gccaatctca
     4021 agacgcgcaa cgatcgcggt gagatggtgc cgatcggctc actggtcaaa ttgaggccca
     4081 cctacggccc ggatccggtg ctgcgctaca acggctatcc cgccgccgac tttttgggcg
     4141 aagccgaccc gaccaaattg tcctcggcgc aggcaatgga caaccttcgc cagatcgcgg
     4201 cgaaggtgct acccaacggt atgactatcg aatggaccga cctgagcttt caggaagcct
     4261 cgcaggggag ggcggcgctg ctggtgtttc cggtcgccat cttgttggcc ttcctcgtgc
     4321 tcgctgcgct ctacgagtcc tggaccttgc cgctcgcagt gattctgatc gtgccgatct
     4381 gcatgctctg cgcgctggcc ggcgtcaaac ttactggcgg cgacaacaac accttcgtgc
     4441 aggtcggcct ggtcgtactg atgggcctgg cctgcaagaa cgcgatcctc atcgtcgaat
     4501 tcgcgcgcga gctggaactg cagggcaacg gcaccatcca agcggcactc gaagcctgcc
     4561 gcttgcgatt gcgcccaatc gtcatgacgt cggtggcctt cattgccggc acggttccac
     4621 tcgtgctttc gcacggcgcc ggcgccgaag tgcgatcggc cacggggatc accgtgttcg
     4681 ccggcatgat tggcgtgacc ctgttcggcc tgttcctcac cccggtgttc tatgtcggct
     4741 tgcgtctatt gtcgggacgc aaactgatca gccatgacca aacaatgccg cgcggtgccc
     4801 ttgaaaacgc gtatagccag gtcgaggcgg cccgttaagc gcgctgatcg gccgagcgcc
     4861 ttccacccga cggctggcga gattcagaga gaccgaaagc tatgcggcac gtccgcggtc
     4921 gccacaagag ttccatagct gaaccaggtt gtcttcgggc atctggcgcg ccgcccttgt
     4981 gattcgccaa taatcccctg accatgtttt gcttgaggtt ggaacagtgt cgaaattcac
     5041 ctctccagac gactattgga tgaaggtgcc gcttcagggg gaaaaaaaac gaaaaggaga
     5101 aatcccatgg aattcatgat tctgatgatc ccgtctgttt accgcaacaa caagaaactc
     5161 ggaccgaact ttgttcccga ccctaaagtg attgaggaaa tgaccaagtt caaccaagag
     5221 ttgtcaaaga gcgtcaaact tctgtcgctg aacggtctac atccgctgac caatggcgca
     5281 cgcttgtcgt tttcgaacgg caaagcgacc gtgactgacg gcccgttcat cgaaacgaag
     5341 gaagttctcg gcggctattg gctggttgag gcgaagtcga aagacgagtt ggtcaagctc
     5401 atgcagaact gcccggccga agaaggagac atcatcgaga tccggcaaat cttcggtgac
     5461 gaagattttg cgataaaaaa ataagggcgg cctgaaatac atctgcctgg gctaaaagga
     5521 gcaactcggt ccatggacgc ctgcttcgcc tacgaagaag aactgcagaa aaagacgacg
     5581 tacaatgacc gctcagtcat tgcacaccga aactagaggc gacctgcggt ttacgaatca
     5641 gtgaaaggga gttgctcaag tgcttgtcac ttgggcaaat tcccttccac tagctgcgtt
     5701 tgccttcaat gaccattcta ccgagctatc gcttcctatc cttccgtacc cactttgtcc
     5761 ctattcctaa cattggaata acaccaaccc ggggtagggc tgtaaaattt ccgaccgagt
     5821 ccccctgaat ttgaactgac cggcttaaat gcatagggaa aagaaaaccg aaggggcccc
     5881 aagcaggcta cacgagacac ccagcacgaa gcacgaaaag caagttgcgc gggagaattt
     5941 tcgaaggatg cgacgcagat acgaggtctt gcccgagtga acgctactag aacggtcaat
     6001 acaacaccga agggagaaat gcaccggtcg ggttctgata cagaccgcgg ctcatgagcg
     6061 tcatgggctg gacgacgcgc agaccttgag tcagacacca ccgtagtaat tccgcattgc
     6121 gggttgggag caagaaccct gggccgacaa acgtcggagc ggtaccgatc agcgccttga
     6181 ggtcttcatt cgtccggccg acggcgtggg cgaagaaccc gatattggtg gcataaccag
     6241 tgattcgacc accgtactcg actattctgg ccgttccatc agcgatcgca tacgcaagtt
     6301 caccacgccg atcgtgccca tgcacattga ggcacagttc attgcactcg ccgaggtcat
     6361 cgagcgtcgc ggagcgcacg cggtagccgg gcaactcaaa atttagcggc ggtccctgca
     6421 agactgagag gggttcgcgc gcatggaagc cgagcttggt gtacacggat agcgagcggt
     6481 tgtgataagc ggtttggacg agccggactc cgacatgctg tcgtgcgtgg gcccgttcca
     6541 gtacgcgctc catcaattga cgtcccacgc tacagttttg cgcggctgga acgacggtaa
     6601 tcggacccac gcccgcgatg gtgtcgccct cccagaggaa gttgctgccg actactcgat
     6661 catcaatctc cgcaattact gagtagacat cagcgcgtga cagcaccatc gagaacaagg
     6721 agattgcgat gtcgggggag ggaaagtcag gagggaaatt gtgctgttcg gcaatgccct
     6781 taaacgcctc ataacagata ctgccgcagg ccgtcgcatc tccggctgta cctgatctga
     6841 gtaccgtttt caccgaccag cctcccggta ggaaactaca ggtcccttca ggaagtagtc
     6901 aagggaaggg ggcggctatg ggagccaagc tctttggtcg gcgctaagaa ccacgaacca
     6961 cgcaacacga gctacgtgat acgtgctgcg tgctgggtgt ggctggatgc catttcatta
     7021 agtgaccgga ttaaaacggc tcgcatacaa atcgctctct tacactgtga aagtcattca
     7081 gggtcctaaa gctacactta ttagatgaat cagaccagca cgcgccaagg ggaactgttg
     7141 gcgctccagt ggaacgatgt gaacctcact ggacgattct gcgaagtgcg ccggaatttc
     7201 actcacgggc ggctcacgac ccccaagagc ggcgaatccc gtcgggtcga cctctctcga
     7261 gaattgacgg cgaccctgga ggccttgcag ttggatcggc agttagaagc ggccacgcaa
     7321 ggtcaggacg ccgcgcctcc ctgggtcttt tgtgaccgtc agggcaagcc cttccatcag
     7381 aactgggtgc ggcttcggtt cttccggttg ttaaagggtg ccggtgtgcg ccaggtacgc
     7441 ttccatgatc tccgccatac gttggcctcg ctcttgctcc agaacggcga gagtcccgtg
     7501 tacgtgaaag agcagacggg ccattgctcc atccaaatca ccgtggatca gtctggccat
     7561 ctcattccgg gtgggaacgt ccgagctgta gaccggctcg atacgggcgt ggaagaaagg
     7621 ccgttggtgg ccgaatctgc aacctccctg caaccggcca cagtgtttcc gggacccctg
     7681 tcatcggatc agccgaaatc tccagtagtt acgaggaagg tgtctggggt ggatgacggg
     7741 tttcgaaccc gcgacctccg gatccacaat ccggcgctct aaccaactga gctacaccca
     7801 ccgtgcaggt gctgagagga agaacagagg aaattggatc ttaacaaagt gagaagaacg
     7861 gccgcaactc attggccgtc agcttctggc atcaaacgct gcctgcattt cactcagaac
     7921 cgccgcggcc gcggcagcgg gatcggcagc ggctcgaatg ggacgaccaa tcacgaggta
     7981 atcggcacca gccgcgatgg tttgggttgg agtcgtcgac ctcgcatgat cgtcaacgtc
     8041 ctcaccggcc gggcggacgc ctggagtcac gatgacgaac cgtggcccga ccttcggtct
     8101 caacaccccc ggttcttcgc ctgacgcgac cacaccgtcg caacccatct ccgccgccaa
     8161 caacgcgcgg gccgtcacga gctcctgcac tgtgtgctgg atacccatct cgcgtaagtc
     8221 atgactgtca aaattggtaa ggaccgtcac ggccaacaac ttgagggccg aatcgccccg
     8281 acccttgacc gcagccgcaa gcgctttacg attcgcatgg acggtcagaa actcgacgcc
     8341 catcgctgct actcgttccg tggcccgccg aaccgtttcc tcgatatcca gaaacttcaa
     8401 atccagaaag acgcgcttgc cccgcttcag gagacgctgc acgatttccg gtccagccgc
     8461 tgtatagagc tcgagcccca ccttgacgaa cgtgacctga tcctgcacct gatccatcaa
     8521 acgttcggca tccaccgccg aaggtaaatc caaggcgaga atcaaccggt cacgcgcgac
     8581 gaccttcacc gcaaaatcct cccacaccgt ttttataggc tctgctcacc ttgacgatcc
     8641 gccgaagcct atgctacaaa tgcgctcctt tgtacaggag tttgctatgc cagtcatcca
     8701 taaatcgacg atacggcggg cccgtcaggc agaaaagcgg caggctcgca accgcgccgc
     8761 tctgagtgcc gtgaaaagcc tggtgaaaaa ggtgcacgcg gcggtcactg acaagaaggc
     8821 cgacgaagcc aaggcctctc tgcaaatcgc cacatcggct ctcagtaaag ccgtcagcaa
     8881 gggcgtgctc aaaccaaata ccgcatcccg ccgcatttcg agactgacca gacacgtcaa
     8941 ttctttggtc gcctcacgct aaccgttcga acattcgaca aggtcctccc tcgcggcgcc
     9001 ggagcagcgg gagagggcga gcgtgtcggc tgtgggcggt tacacagctt gaacatcagc
     9061 tgatcgagca gcatcgccgg cttccctccg ctaccaccct tcagtttttg atcagtctcg
     9121 aaaaataagc ggaacgcctc ccgcaagtgt gtgtcagaaa attgcccaag gaaggggcgg
     9181 attttgtaag ggtccatgcg caggagcctg gctgcctcgc cttcacggcc agcctgtttg
     9241 accagttcct tgaccttcca gagccgccga tactgccaga ccagagaacc cagaatccgc
     9301 aagggcgctt ctcccgcctc tagattgcga gccaggatgg ccagcgctgt cccccgctgc
     9361 tgtgcgccga tcgcggcggc caggtcaaag accgaggcgc ccggt
//
LOCUS       DASTQY010000078         5395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_350378, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000078 DASTQY010000000
VERSION     DASTQY010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350378"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFR82_02470"
     CDS             complement(<1..203)
                     /locus_tag="VFR82_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500489.1"
                     /translation="MAEASSPVQQIQTAYPDFIAEYPSDRLVQYRIDKSRLAELTCFL
                     HEKLRGRLALLFAVDCRPLEEKY"
     gene            complement(213..1727)
                     /locus_tag="VFR82_02475"
     CDS             complement(213..1727)
                     /locus_tag="VFR82_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HEU4500490.1"
                     /translation="MTGQTVTMAVGLLLLAPLVAGWLSLLIKGSRWLHVTNLISIGTL
                     VGAEAIIARAVFTQGSITTFGEIVYIDALSSVILFIIGTVGLACSLYMRSYMDEQVAR
                     GVIAPKRLNLFFFLFHMFLLAMVLATVANSLGVQWVAIEATTLATTFLIAFWRRRESL
                     EAGWKYLILCSVGISLALFGVVLMYYSSLRVLGDVSSALNITQLQQVAAQLDPHILKL
                     AFVFLFVGYGTKVGLVPMHSWLPDAYTEAPAPVAAMLAGVLETVAVYAILRCRAIADQ
                     ALSPDFTGGLLALFGLISFALAAFFILLQHNYKRLFAYSSIEHMGLAMVGFGVGGPIG
                     NFGGLFHLVNHAFAKSLAFFAAGNIHRRFKTVEIDQVRGLTTVLPWSALALLISGLAL
                     AALPPFALFASEIQIITALGSSGLPREWTQPDTRFPVVILLLCSVVAFAGFLYRITGM
                     VWGAAPKDVVQGERWSVGHVPIILLGSVLIAFCLALPASLRQFFEMASTLLLSR"
     gene            complement(1724..2392)
                     /locus_tag="VFR82_02480"
     CDS             complement(1724..2392)
                     /locus_tag="VFR82_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HEU4500491.1"
                     /translation="MSWLTPHIGTQLVDFGSAMLLLTCFAIVAQRRLSACVDLFALQS
                     LFLAMTAALVAFLTGTRHIYIAAALTVVVKVIVIPRVLKSVIERLNVTRELVMKVNVP
                     ASLLICGALVMIAFFITQPIIPFGHLLTRDSLAIALAIVLIGFFTMISRQKAVTQLIA
                     FLVMENGLFLGATAATYGMPLIVELGVFFDVLVAALIAGIYTNRLQDAFDSVDTTHLS
                     ELKE"
     gene            complement(2395..3363)
                     /locus_tag="VFR82_02485"
     CDS             complement(2395..3363)
                     /locus_tag="VFR82_02485"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEU4500492.1"
                     /translation="MVKDQSILQAIALTLSEAIVLIAVSPAIVGLIRKVKARLQCRQG
                     AGIFQPYADLRKLFLKQAVVSTTTSWIFTATPYIVFASTITAGLLVPVFTSQMPLNFA
                     GNIIAFVYLLALGTFFLILAGLDAGSPFGGMGSSREAIVASLTDPAMIMAIFAIALTA
                     GSTNISTIVHETVLLEGIVTDPSSRVMALAALFLVTIAETGRVPVDNPATHLELTMIH
                     EAMVLEYSGRYLALLEWASGIKLLVFLTLIANVFAPWGIATSLTPTALSIGLGVYVVK
                     VAGLAVLIGTIECMFAKLRLFRVTDLLGVAFILALLSLLFFYILRG"
     gene            complement(3373..>5395)
                     /gene="hyfB"
                     /locus_tag="VFR82_02490"
     CDS             complement(3373..>5395)
                     /gene="hyfB"
                     /locus_tag="VFR82_02490"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HEU4500493.1"
                     /translation="ILLLFTLIGYAAGLLLPLCIPNKPRLQNFVAHGLAGFAGTGGIC
                     LGIENLFMPEPFTMSVPSTLPLLTFAIRLDPLASFFLLMISLVGLAASVYALGYVTRH
                     YGRDSIALLGSLFNGFLLSMTLVILADNGFFFLIVWEVMSLFSYLLVVTEHEKTDVRY
                     AGLFYLIMTHVGTAFIILTFLLLFQGSGSFSFDAFRHPEQPLTDGMRTIAFLTALIGF
                     GTKAGIVPLHVWLPYAHPAAPSHISALMSGVMIKTAIYALIRVYFDFFGGQFPWWWGF
                     TVLVIGAFSALLGVMYALMEHDLKSLLAYHSVENIGIILLGIGAGMIFHSYGLEELAA
                     LGLIAGLYHTINHAVFKALLFFGAGSLLYATHTRNMEEYGGLLRRMPWTGACFLIGAV
                     SIAALPPTNGFVSEWLVFQSLFLSYQMPSLLMKLMLPIAAAMLALTGVLALTCFAKAF
                     GISFLALPRSSHAKHAEEVPWSMRMGMGFLSAICIALGLAPMLVVPVLDRVTASMIGI
                     SIAEKMLALGGWALAPVNVEFSSLSSPVLGLVLAATATLGLALVALYGGARVHRYSRT
                     WGCGLNLTSRMEYSAIGFVQPIKRVFSTIYQPTVKLETEFLEESRYFAKRRRFEFHIE
                     PMFQKYLYDPVITFTSGLADRLRIVQAGSLHLYLAYMFVTLVVLLLVAL"
BASE COUNT         1449 a         1438 c         1559 g          949 t
ORIGIN      
        1 tcgtatttct cttcgagcgg acgacaatcg acggcgaaca gcagtgctag gcgcccgcga
       61 agcttctcgt gaagaaagca tgtaagctcc gcaagccggc ttttatcaat gcgatactgt
      121 accaggcggt cagatggata ctccgcaatg aaatcaggat aggccgtctg tatttgttgg
      181 acaggcgatg atgcttccgc caccgttcac tcctatcggc tcagtagcag ggtcgaagcc
      241 atttcaaaaa actgtcgcaa cgacgccggt aaagccaggc agaatgcaat gagtactgag
      301 cccagcagaa tgattgggac atgacccact gaccatcgct caccctggac cacgtccttc
      361 ggtgcagcac cccaaaccat gccggtaatg cgatagagaa agccggcgaa cgctacgaca
      421 ctacagagca gcagaatcac gacaggaaac ctcgtgtcag gctgtgtcca ttcacgtgga
      481 agacctgacg acccgagagc cgtgatgatc tgaatctcgc tggcaaatag cgcgaaaggt
      541 gggagcgcag cgagcgccag tcctgaaatc agcaatgcta aagcagacca gggcagcacg
      601 gttgtcagac cgcggacctg gtcgatctct acagtcttaa aacgccggtg aatattgcca
      661 gccgcaaaaa acgcaagcga cttggcgaag gcatggttga ccaggtggaa taatccgccg
      721 aagttgccga tgggcccccc aaccccgaat ccaaccatcg ccaaccccat gtgttcgatg
      781 ctggaatagg cgaacagtcg cttgtaattg tgttgtaaga ggatgaaaaa ggccgcaagc
      841 gcaaatgaga taagcccgaa cagcgccaac aacccgccgg tgaaatcggg agagagggcc
      901 tggtcggcga tcgcgcggca tcggagaatg gcataaaccg ccaccgtttc aaggacgccg
      961 gccagcatgg ccgcgaccgg ggctggggct tcggtgtagg catcgggcag ccagctgtgc
     1021 atgggcacaa gaccgacttt ggttccgtat ccgacaaaga gaaagacgaa tgctaatttc
     1081 agaatgtgtg ggtcgagctg tgcagccacc tgctggagtt gggtgatatt gagagcggag
     1141 ctgacatctc caagcacgcg aagggaggag tagtacatca ggacgacgcc gaatagtgcc
     1201 agcgaaatgc cgacggaaca gaggatcaaa tacttccaac cggcctccaa cgattcgcgg
     1261 cgccgccaga aggcgatgag aaatgtggtc gccaatgttg tcgcctcgat cgccacccat
     1321 tgcacaccca aactgttcgc gactgtcgcc agcaccatgg ccaataggaa catgtgaaac
     1381 agaaaaaaga agagattgag ccgcttgggc gcaatgacac cacgtgcgac ctgctcgtcc
     1441 atgtatgagc gcatatagag cgagcaggcg agcccgacgg tgccgataat gaacagaatg
     1501 accgatgaca gggcatcgat ataaacgatc tcgccgaatg tcgtgatcga cccttgtgtg
     1561 aacacggctc gtgcgatgat cgcttccgcg ccaaccaacg ttccaatgct gatcagattg
     1621 gtcacatgga gccagcgtga gcctttgatc aacagactga gccagccggc caccaatggc
     1681 gcgagtagga gcaggccgac agccatcgta acggtttgtc cggtcattcc ttcaactcac
     1741 tgaggtgagt ggtgtccacg ctgtcgaacg catcttgaag tcgattcgta tagattcccg
     1801 cgatcaaggc cgcaaccaac acatcgaaga acacgcctaa ctcgacgatc agcggcatgc
     1861 cgtatgttgc agctgtcgct cccaggaaga gaccgttttc catcaccagg aacgcaatca
     1921 actgcgtcac ggctttttgg cgcgagatca tcgtgaaaaa tccgatcagc acaatggcca
     1981 acgcgatggc gagcgaatcg cgggtcaaca aatgcccgaa tgggatgatc ggctgggtga
     2041 tgaagaaggc gatcatgacg agcgcaccac agatcagcag actcgccggc acgttcacct
     2101 tcatcaccag ttcgcgtgtg acgttgagcc gttcgatcac gctcttcagc acgcggggaa
     2161 ttacgatcac tttcacgact accgtcaatg ccgcggcgat ataaatgtga cgagtgccgg
     2221 tgaggaaggc caccagggca gccgtcatgg ccagaaacag cgactgtaac gcgaagaggt
     2281 ccacacaggc ggaaagccgg cgctgcgcca cgatggcaaa gcaggtcagg agcagcatgg
     2341 ctgaaccaaa gtcgacgagt tgagtcccga tgtgcggagt cagccaggac acagttatcc
     2401 tcgcaagata taaaagaaca acaaactcaa caaagccaga atgaaggcga cgccaagcag
     2461 atcggtaaca cggaagaggc gcaacttggc gaacatgcat tcgattgtgc cgatcaacac
     2521 cgcaaggccg gcgactttga ccacatacac ccccagaccg atcgacagag ccgtcggcgt
     2581 caaactcgtc gcaatgcccc atggcgcgaa aacgttggca atgagtgtca agaacacgag
     2641 cagcttgatg ccggaggccc attccagcag cgccaggtag cggccggaat attccaacac
     2701 catcgcttcg tggatcatcg tcagttcaag atgagtggcc ggattatcta cgggaacccg
     2761 gccggtttcc gcgatggtca caaggaagag ggccgcgagt gccattacac gcgacgaagg
     2821 atcggtcacg atgccttcca acaagacggt ctcgtggaca atcgtgctga tattggtgga
     2881 accagcggtc aatgcaatcg caaagatggc catgatcatg gccggatcgg tcagcgaggc
     2941 cacgatcgct tctcggctgc tgcccatgcc gccgaatggc gagcccgcat cgagcccggc
     3001 aagaataaga aagaacgtgc ccaatgcaag caagtagaca aaggcgatga tattgcccgc
     3061 aaagttcaaa ggcatctgcg aggtaaaaac ggggacgagt agcccggccg tgatcgtcga
     3121 agcaaagacg atgtacggag ttgccgtgaa gatccatgaa gtcgttgtag agaccaccgc
     3181 ttgtttgagg aacaattttc taagatcggc atacggttgg aaaatgccgg ctccttgccg
     3241 gcattggagc cgcgccttca cctttctgat cagaccgacg attgcgggcg agacagcgat
     3301 caacacgatg gcctctgaca gggtgagtgc gatcgcttga agtatcgatt ggtctttgac
     3361 catgactctc cgttacaggg ccaccaacag cagcaccacc aaggtcacga acatataggc
     3421 gagatagaga tgaagactgc ctgcttggac aattcttagc cggtcggcca atccggacgt
     3481 gaacgtgatg acggggtcgt agaggtactt ctgaaacatc ggttcaatgt gaaattcgaa
     3541 tcgccgccgc ttggcgaaat agcgcgactc ttcaagaaac tccgtttcga gcttcaccgt
     3601 cggttgataa atcgtgctga agactcgttt gatcggttgc acgaagccta tcgcgctata
     3661 ttccattcga gaggtcagat tgagaccaca cccccaggtc ctgctgtatc gatggactcg
     3721 ggcaccgccg tagagcgcga caagggcgag ccctagagtc gctgtcgctg ccaggaccag
     3781 gccaaggaca ggggaggaga gactcgaaaa ttctacgttg accggtgcca aggcccatcc
     3841 gccaagcgcc agcattttct ccgcgattga gatgccgatc attgaagcgg tcacgcggtc
     3901 caagacgggg accaccagca tgggagccag ccccagagcg atacagattg ctgacaggaa
     3961 acccattccc atgcgcatcg accaaggcac ttcttcggca tgtttagcgt gggaactgcg
     4021 cgggagggcc agaaaggaaa tcccgaaagc tttggcgaaa caggttagag caagtacgcc
     4081 ggtaagcgcc agcatggcag ccgcgattgg gagcataagc ttcatcagta gggaaggcat
     4141 ctggtagctc aagaagaggc tttggaagac cagccattcg ctgacgaaac cattggtcgg
     4201 cggcagcgcg gcgatcgaga ctgcgccgat gagaaaacag gcgccggtcc atggcatgcg
     4261 acgcaggagg ccgccgtatt cctccatgtt gcgggtatgg gtggcgtaca gcagtgagcc
     4321 cgcgccaaaa aacagcaacg ccttaaagac agcatgattg atggtgtgat agagacccgc
     4381 gatgagccct agcgcggcca actcctcgag accatagcta tgaaaaatca tacctgcgcc
     4441 gatcccgagc aaaatgatcc cgatgttttc gacgctgtga taggcgagga ggcttttgag
     4501 gtcgtgctcc attaatgcgt acatgacgcc gagtaatgcc gagaaggcgc cgatgacaag
     4561 cacggtgaat ccccaccacc agggaaactg tcccccgaaa aagtcaaagt acactctgat
     4621 caacgcatag atggcggtct taatcatgac gcccgacatg agtgccgaga tatgggaggg
     4681 tgcagccgga tgggcatacg gcaaccagac atgaagcggc acaatgcccg ctttggtccc
     4741 gaacccgatc agcgctgtca ggaaggcaat cgttctcatg ccgtccgtca agggttgctc
     4801 cggatgacgg aaggcatcaa aagaaaagga tccacttcct tgaaagagca gaagaaacgt
     4861 caggatgatg aaggcggtcc ccacgtgggt catgatcagg tagaacaaac cggcataacg
     4921 tacgtcggtc ttctcatgct ccgtcaccac gaggagatag gagaacaagg acatcacttc
     4981 ccaaacgatc aggaagaaga atccattgtc ggcgaggatg accagtgtca tcgaaagcag
     5041 aaaaccattg aagagtgagc cgagcaacgc gattgaatcc cgtccgtagt gtctagtcac
     5101 atatccgagc gcatagacgg aggcggccag gccgaccaac gaaatcatca gcagaaaaaa
     5161 tgaggcaaga gggtctagtc ggatggcgaa ggtgagaagg ggcagagttg agggaacaga
     5221 catcgtgaat ggttcgggca tgaaaaggtt ttcgatgccg aggcaaatcc ccccagtccc
     5281 tgcaaagccc gcaaggccgt gagcgacaaa attttgcagc cttggcttat tgggaataca
     5341 gagtgggaga agcagtccgg ccgcataacc gatgagagtg aagagaagca gaatc
//
LOCUS       DASTQY010000079         5947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1559249, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000079 DASTQY010000000
VERSION     DASTQY010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559249"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VFR82_02495"
     CDS             <1..916
                     /locus_tag="VFR82_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HEU4500494.1"
                     /translation="VVRSGRGVELKDGTAYTVTVAMRQGEERAVGPLLQATPEANWFN
                     WNQLNNLMLALGFGGLVFYSIRHAKRNDIFLRRIPGLDAVDEAIGRSTELGKPILYLT
                     GAHDMSDPSTIAAAVILGRVAKRTAAYETELLVPHRDPITMAVCQEITKQAYLEAGKP
                     DLFKEDANFFITADQFSYTAAVDGIMLRKKPAANFFMGSYFAESLLLTETGASTGAIQ
                     IAGTDSDHQLPFFVTTCDYTLIGEELYAASAYLSREPIQIGTLRGQDIGKAIVLTVIM
                     IGTLIATIGALTGSSWPQLFLDLFKDLK"
     gene            913..1563
                     /locus_tag="VFR82_02500"
     CDS             913..1563
                     /locus_tag="VFR82_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500495.1"
                     /translation="MIFLRRRLPLLITMITGLLFAAQYYVPHPASELLLTSATKWLQI
                     IGGFALVLGVTSLFHQHAVKIRRQEAGWGYSLVLYAGMLVTIAVGLWAGGKESLDGTM
                     TAFGWIYTYMMVPLQGTMFAILAFFIASAAYRSFRARSREAAVLLIAAVIVMMGRVPL
                     GEYLIPLSGDVSQWILNVLNASVRRAILIGVSLGAIALSLKIIFGVERSYLGGGKE"
     gene            1697..2527
                     /locus_tag="VFR82_02505"
     CDS             1697..2527
                     /locus_tag="VFR82_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500496.1"
                     /translation="MTFSERMLRIDRRIIFMVIGLCTLLPLLYPVGLPIKISPEVRNV
                     YDYIESLPERSVFLLSIDFDPASKPELHPQAIALLRHAFRKNLRVVAMTLWVSGTGMA
                     DQLLKQVSSEMGRERGRDYVFLGWSPGGSAVIINMGQDLYTAFPSDYEGKPTRGIPVL
                     EGVHSLKDVTYAISLGAGNPGVEAWYVYGKDKYKFELGGGCTGVIAPGLYPLLRSGQI
                     NGLIGGLRGAAEYESLIGQKGRAVAGMDAQSATHLAIIVLVAMCNLFYFSLLQQKGRG
                     "
     gene            2559..3308
                     /locus_tag="VFR82_02510"
     CDS             2559..3308
                     /locus_tag="VFR82_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500497.1"
                     /translation="MDATILGAWMATGLTLFIFSFLYQDNPLFKLAEHLYVGVSVGYT
                     IVKAYDTVIVHLIVKPIVEEGQIALLIPVAIGLLMLTRYVPRGAWMSRYAFAFIVGLG
                     SGLAIPRTISSFILKQVEDTIRPLLAQAGGDGLTFSLNLLNPSSSLNAVIILVGVSTV
                     LFYFFFSIEHSGPGKIVARTGIVFLMIAFGAAFGYTVMARMSLLIGRLTDLIEFSDVS
                     YGRPTWWLLILTIGVLVLLARRVPNRPPDLP"
     gene            3380..4621
                     /gene="amrB"
                     /locus_tag="VFR82_02515"
     CDS             3380..4621
                     /gene="amrB"
                     /locus_tag="VFR82_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEU4500498.1"
                     /translation="MTTGTAKDPQQYPVLRNLQFSPIKEGEEQYIVLWDPSGLSKEKL
                     VLPLNYFFIIQHFDGEHSLQEIGALYLKRFGEFLMPNKVEQLIGDLDVKLFLEGERTE
                     GAKEQARLAYRQSPIRPAAFAGKVYEADGAKLKKQIDGFFASKEGPDFKPSDNKGKLI
                     KGLLAPTYDLKQAGPIYAWAYKEFQQAQQPDVVVIIGTAHAGLEHVFAVTDKDFETPL
                     GVVAVDRAITDRLKAAVPDYFSEEVAHLSEHAIEFQLPFLQTTAGSTTPCSIVPILSS
                     FSAMSLTDPSVRVAVDRFLAALNEVIADSGRTVCVIAAGDLAHLGMRYGDSAPPTDFS
                     FHRAMQYDLEMLKFVEELKADEFAGYIQKEKDQRRISGFSPIYSLLHLIQAERGQVLR
                     YDRGIADQYNSTVTYASMSFF"
     gene            4726..>5947
                     /locus_tag="VFR82_02520"
     CDS             4726..>5947
                     /locus_tag="VFR82_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEU4500499.1"
                     /translation="MRIRKIRGRIVLAIVLVGCIPLVIGLVLAYVSGMQSLRDVIGGN
                     LQAVAVQAADRVTMLVQGEVRNARLLASAPLRVRLPVQVANSDYPPDQESARLLIGHR
                     ALLWEQGGDHAASLLNAELSRFLLETKIRDGEKVIGIMILDRYGALVAASSEPDKYFF
                     GNESWWSTLGTGGEQLYLSGRIPAEEGAFRASDETLDIAIPILDDRQQQMIGAVIASY
                     RFDSLFAMIAQIQIGQTGHAMLFDAAGQPLVCPILARDAHRIPSQLMAMIVSSDPGWA
                     VAEDDGHGASDTVVGYAPVNGLQLPDNSWHIFVRQHPSESYAPIHDQVRNLAAIGLVM
                     LGLLWAMGRYVATRIARPIQLLHSGVEAISQGTYDQPINIKTGDEFEDLSAAVHRMAD
                     RLKASRAELEGLNQD"
BASE COUNT         1171 a         1656 c         1766 g         1354 t
ORIGIN      
        1 tgtggtgagg agcggaaggg gtgtcgaact gaaggatgga acagcgtata cggtgacggt
       61 cgcgatgcgc cagggcgaag agcgggcggt tggtcctctc ctacaggcca cgccagaagc
      121 gaattggttc aattggaacc aactcaacaa cttgatgttg gccttgggtt tcggaggcct
      181 cgtgttctat agcatcaggc atgcgaagcg aaatgacatt tttttgcgtc gcatccccgg
      241 tctggacgcc gtcgatgagg caatcggccg ctctaccgaa ttgggcaagc cgattctgta
      301 tctgacgggt gcccatgaca tgagcgaccc ttcgactatc gcggctgcgg tgattctcgg
      361 gcgtgtggcc aagcgcaccg ccgcctatga aacggagtta ctggtgccgc atcgtgatcc
      421 catcacgatg gccgtgtgtc aggaaatcac caagcaagcc tatctcgaag ccgggaagcc
      481 cgatctcttc aaggaggacg caaacttttt catcactgcc gaccagttca gctatacggc
      541 cgcggtggac ggcatcatgc tgcgaaaaaa acccgccgcg aattttttca tgggctcata
      601 tttcgccgaa tcgctgctcc tgactgaaac cggagccagt accggtgcga tccaaatcgc
      661 cgggacggac tcggaccatc aactgccctt cttcgtcacc acctgcgact acacgctcat
      721 cggtgaagag ttgtatgccg cgagcgccta tctctccagg gagccgattc agatcggcac
      781 gctgcgcgga caggacatcg gaaaggcgat cgtcttgacc gtcatcatga tcggcaccct
      841 catcgccacg atcggggcgc tgaccggttc gtcatggccc cagctgttcc tggatctctt
      901 caaggatctc aaatgatctt cttgcgccgc cgcctgccct tgctcattac gatgatcact
      961 ggtctcttgt ttgccgccca gtactatgtg ccgcatccgg cgtccgagtt gcttctgaca
     1021 tcggccacca agtggctgca gatcatcggc gggttcgcgt tggtcttggg ggtgaccagc
     1081 ctgtttcatc agcatgctgt gaagatcagg cgtcaggagg caggctgggg atatagcctc
     1141 gtgctctatg ccggcatgct ggtcaccatt gccgtggggc tctgggccgg tgggaaggag
     1201 agtctggatg gcaccatgac ggcattcggc tggatctata cctatatgat ggtaccgctg
     1261 cagggcacga tgttcgcgat cctggccttt tttatcgcgt ctgcagccta ccggtcgttc
     1321 agggccagga gtcgagaggc ggcagttctg ttgattgcgg cggtgatcgt catgatgggc
     1381 cgtgttccct taggcgagta tttgatcccg ttgagcggcg acgtctcgca gtggatcctc
     1441 aatgtgctga atgcctcagt ccgtcgtgcc attttgatcg gcgtgagcct tggcgctatc
     1501 gcgctctcct tgaagatcat cttcggcgtc gaacgttcct acttgggcgg agggaaagag
     1561 tgagaactgc atccttccgc tgcgggaatg tagcgcaagc cgcccagtgt tcgcatgtcg
     1621 ggatgggtga gtgcgaaccg tcctgctcgg cgccgcccct catagatggg cggcaggaag
     1681 cgagaggagg tatggcatga ccttctccga acgaatgctg agaatcgacc ggcggatcat
     1741 ctttatggtg atcggactct gtacgctgtt gccgttgctc tacccggtgg ggttgccgat
     1801 caagatttct cccgaagtgc gcaacgtcta cgattacatc gagtcgttgc ccgaacgatc
     1861 cgtgttcctg ctctctatcg attttgatcc ggcatccaag cccgagctgc atccccaggc
     1921 cattgctttg ctgcgccatg cgttccggaa gaatttgcgc gttgtcgcca tgacgctctg
     1981 ggtctcaggg accggaatgg ccgatcagtt gctgaagcaa gtttcgagcg aaatggggcg
     2041 ggagcggggg agggattacg tgttcctcgg atggagcccc ggtgggtcag ccgtgatcat
     2101 caatatgggt caggatctgt atacggcatt tcccagcgac tacgaaggta agccgaccag
     2161 gggtattccg gtactggaag gcgtgcatag cttgaaagac gtcacctatg ccatcagtct
     2221 cggcgccggc aatcccgggg tcgaggcctg gtatgtgtac gggaaggaca aatacaagtt
     2281 cgagctgggc ggaggctgta ccggcgtcat tgcacccggc ctgtatccac tcctccgcag
     2341 cgggcagatc aacggtttga tcggaggact gcgcggggcg gctgaatatg agagcttaat
     2401 cgggcaaaaa gggcgggcgg tggccggaat ggacgcgcag tcggcgaccc atctggcgat
     2461 cattgtgctg gtcgcgatgt gtaatctttt ctatttctca ctcctgcagc agaaaggccg
     2521 aggatgaggc gcagcctcaa tcggtgtcgg tgactgatat ggatgccacc atccttggtg
     2581 catggatggc cacggggctg acgctgttca tcttttcttt tttgtatcag gataatccgc
     2641 tcttcaagct cgccgagcat ctgtacgtcg gcgtgtcggt cgggtacaca atcgtcaagg
     2701 cctatgacac cgtcatcgtg catttgatcg ttaaaccgat tgtcgaggaa gggcagatcg
     2761 cgctcctcat tccggtggcg atcggcttgc tcatgctgac gcgctatgtc ccgagggggg
     2821 cctggatgtc gcgctatgca ttcgccttta tcgtgggact cggcagcggg cttgcgattc
     2881 cgcgcacgat ttcctcattc atcctcaagc aggtcgagga tacgatacga ccattattgg
     2941 ctcaagcggg cggggacggc ctgacctttt cgctgaatct cctcaatccg tccagtagcc
     3001 tcaatgcggt gatcatcttg gtcggcgtca gcacggtcct cttctatttt ttcttttcca
     3061 ttgaacatag cggcccgggc aagattgtag cgaggaccgg gattgtcttt ttgatgatcg
     3121 cgttcggtgc ggcattcggg tacaccgtga tggcacgtat gtccctcttg atcggccgac
     3181 tgaccgatct catcgaattc tccgatgtct cttacggtcg acccacatgg tggctgctga
     3241 ttttgaccat cggcgtgctc gtcctgttgg ccagacgcgt ccccaaccga cctcccgatc
     3301 ttccctaaag accgtcgatc cagcagttgc aggtctggaa agtggttcgt tagaatgccg
     3361 ctttgccttg tgaggagaca tgacgaccgg caccgccaaa gatcctcagc aataccccgt
     3421 cttgcggaat ctccagttct ccccgattaa agagggagaa gagcaataca tcgtgctctg
     3481 ggaccccagc ggattgagca aagaaaagct tgtcctgccg ctgaattact tttttatcat
     3541 tcaacacttt gatggcgagc attccctgca ggagatcggg gcgttgtatt tgaagcgctt
     3601 cggtgagttt ctgatgccca acaaggtgga gcaattgatc ggcgatctcg acgttaaact
     3661 gtttctcgaa ggagagcgca cggaaggcgc gaaggagcag gcccgcttgg cctaccggca
     3721 atctccaatt cgtccggcgg cgttcgcggg gaaagtctac gaagcagacg gagccaaact
     3781 caagaagcag atcgatggct tctttgcatc caaagaaggg ccggatttca aaccgtcgga
     3841 caataagggc aagctcatca aaggtctgct tgcgccgaca tatgacctca aacaggcagg
     3901 ccccatctat gcctgggcct ataaagaatt ccaacaagct caacaaccgg acgtcgtcgt
     3961 gatcatcggc acggcccacg ccggccttga gcacgtgttt gccgtgacag acaaagactt
     4021 cgaaacccca cttggagttg tggcggtgga ccgtgcgatc acggaccggc tcaaggctgc
     4081 ggtgcccgac tatttttccg aagaggtcgc tcatctctcg gagcatgcca tcgagtttca
     4141 attaccgttc ctgcaaacga ccgccggctc gacgacaccg tgctccatcg tgcccattct
     4201 ctcctccttc tcggcgatga gcctgactga tccatcggtg cgcgtagcgg tcgatcgatt
     4261 ccttgcggcg ttgaatgagg tgattgcgga ctccggccgg accgtttgcg tgattgccgc
     4321 cggagacctc gcccatctgg gaatgcgtta cggcgacagt gccccgccga ccgatttctc
     4381 cttccatcgg gccatgcagt atgatctcga aatgctgaag ttcgttgagg aactgaaggc
     4441 agatgagttt gccggataca ttcagaaaga aaaggaccag cgccggatct ccggcttctc
     4501 gcccatctac agcctgcttc acctcataca ggctgaacga ggacaggtgc ttcgatacga
     4561 ccgaggtatt gccgaccagt acaactcgac ggtgacttac gcgtccatgt cgttcttcta
     4621 gccgcctgcc cgttcgacct ctctccagcc gagtcaccgt ttaccgatgg tggagtttcg
     4681 acatcagcgt ttcgtacaat accctggagt gaggggagac agccggtgag aatacgcaag
     4741 attcgtggac gcattgtctt ggcaattgtc ctggtgggat gtatccccct ggtgatcggc
     4801 ctggtgctgg cctatgtctc cggtatgcaa tcgctgcggg atgtgatcgg cggcaacctg
     4861 caggcagtgg ccgttcaagc tgccgatcgt gtgaccatgt tggttcaagg ggaggtgcgc
     4921 aatgctaggt tgctggcctc ggcgccctta cgcgtgcgct tgcctgttca agtggccaac
     4981 tcggactatc ctcccgatca ggaaagcgcg aggctcttga tcggccaccg ggccctgctc
     5041 tgggaacaag gcggagacca tgccgcctcc ttgttgaatg ccgaactctc ccggtttctc
     5101 ctcgagacca agatccggga cggagaaaaa gtgatcggaa tcatgattct ggaccggtat
     5161 ggagcgctcg ttgcggctag ttccgagccg gacaaatatt ttttcgggaa cgagtcctgg
     5221 tggagcactc tgggtacagg gggggaacag ctgtatctca gcgggcgaat tcccgcggaa
     5281 gagggggcat tcagagcctc cgatgaaacg ctcgatattg cgatccccat tctcgacgac
     5341 cggcagcagc agatgatcgg ggcggtgatc gcgtcttacc gtttcgacag tttatttgcc
     5401 atgattgccc agatccagat cggccaaacg ggccacgcga tgctcttcga tgcagcgggc
     5461 caaccgctgg tttgtccgat tctcgcgcgc gatgcccatc gcatccccag tcagctgatg
     5521 gccatgatcg tgtcctccga tccgggctgg gccgtggcag aggacgatgg tcatggcgcg
     5581 tccgacactg tggttggata cgcgccggtg aacgggcttc aactgcctga caattcctgg
     5641 cacatctttg tcaggcaaca tccgagtgaa agctatgcgc cgattcatga ccaggtgaga
     5701 aatctggctg ccatcggtct ggtgatgctc ggcctgttgt gggccatggg ccggtatgtt
     5761 gcgacccgga tcgcgcgccc catccaattg ttgcacagcg gggtggaggc gatcagccag
     5821 ggcacgtacg atcaaccgat taacatcaag accggagatg agtttgaaga tctctccgcc
     5881 gccgtccacc gcatggccga taggctgaag gcctcccgtg cagagctcga aggcctcaat
     5941 caggatc
//
LOCUS       DASTQY010000080        13002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1558850, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000080 DASTQY010000000
VERSION     DASTQY010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 13002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13002
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558850"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFR82_02525"
     CDS             complement(<1..609)
                     /locus_tag="VFR82_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500500.1"
                     /translation="MGGRKRYVSMGMMRRWIWLVWSLTLGMGSGTPTASALEFTADQI
                     TKIDGRTHKAFIYYRDQMWRIEHHSLGPVNVTIVRKDKHLVWLLLSRMKHFKTLPYDA
                     DQDLRVTAGLDGEVSRQEIGTEIREGHPTTLYEVTTKRGEHIEQYYQWVATDLQFPMK
                     LAKKDGSWIVEYHHVKMRSVSDYLFNLPVNFQPLEEFDASPPV"
     gene            complement(623..1618)
                     /locus_tag="VFR82_02530"
     CDS             complement(623..1618)
                     /locus_tag="VFR82_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU4500501.1"
                     /translation="MVLGSPRDVAGDPLRLDDVPMPEPGSGEVLVRVSVCGICRTDLH
                     VVEGELERPKLPLIPGHQAVGLISRLGSGVNERTIGERVGVAWLQGTCGQCGYCRSGR
                     ENLCLSARFTGYQVDGGYAEYAVLPAAFAYPIPPAFSDEEAAPLLCAGIIGYRALRLS
                     QIQPGQRLGLYGFGASAHIAIQLARHWGCEVYVCSLNPSHQALARDLGAAWVGGASDA
                     PPVRLHASIMFAPAGQLVIPALRALERGGTLALAGIHMSTIPPLEYDRDLFGERVIRS
                     VTANTRQDGLDLLREAAAIPIRPRTQRFPLAQANRALQALKSGAINGAGVLTIHS"
     gene            complement(2011..2964)
                     /locus_tag="VFR82_02535"
     CDS             complement(2011..2964)
                     /locus_tag="VFR82_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU4500502.1"
                     /translation="MLLIGAFGIGVVLCGSFLTPISPSSAAGSGSRWARFELNQQDAS
                     GDLQTGKPVALVITLGGVTQGTGPIVALCESILFEPQLITLEPDGESSALKAEVTLDP
                     MPSSRTSVHQKAARIQVTFARARPEKLERFMKRIVYVTLDHQEPSSDTSEPPVVRPEE
                     PSTGDLIVDELQPSGVAPVSTGSLLEEDLMPLGDPGQGKAYWQQVSLLVSRSWARQVR
                     GVRRSPSSETVRVRFKLYPNGRAQLIQIEKGSGAREIDEAGIYAVVNAQPFPPLPEEV
                     GDEPVDVHVRMRTGARSSAREVQSVGNSSNGKLEAPAQPKK"
     gene            3098..4120
                     /gene="tdh"
                     /locus_tag="VFR82_02540"
     CDS             3098..4120
                     /gene="tdh"
                     /locus_tag="VFR82_02540"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEU4500503.1"
                     /translation="MKTAPQPGLTATTCPDPSPGPHEAVIRVKATSLCGTDVHIYNWD
                     PWAHSRIHPPRIIGHEMCGEVVEVGSAVMLVKVGDYVAAESHITCGLCFQCRTGQAHV
                     CRNYKILGVDRDGSFADYVVLPENVLWHTSRAIAPEYASLQEPLGNAVDAALVEDLTG
                     HTVLITGCGPTGLLAAAVARTSGAATIIASDISEYRLGLAKQVGADHVLNAKTETAAS
                     LHEAIDEITDGEGVDASLEMSGNPAALHLAFDTVKNGGRVTLFGIPTGPITCDLANDI
                     IFKGIRVHGITGRRLFGTWYRLAGLFKAGLNIKPIVTHTFPMSDFAQGFDLIKTGQCG
                     KVVLFP"
     gene            4163..5359
                     /locus_tag="VFR82_02545"
     CDS             4163..5359
                     /locus_tag="VFR82_02545"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEU4500504.1"
                     /translation="MADSTLKQIVSEQLASIRAKGLHKSERQLLSPQNADIFVAQGRV
                     LNLCANNYLGLANHPRIIEAVIKGLRDHGYGMASVRFICGTQDIHKELERAISTFLGT
                     DDAILYGSCFDANGGLFEVLLDERDAVISDALNHASVIDGIRLCKAKRFRYAHSDMED
                     LEARLHETRPYRLRLIATDGVFSMDGDLARLDQIVELAQRFDAAVMVDDSHATGVIGR
                     TGRGTPDHFGVADRVDIVTSTLGKTLGGATGGFTAGHAGVIELLRQRSRPYLFSNSLP
                     PPIVAGALQALALVAEGDNLRARLSENAAFFRARLTELGFRLVPGHHPIIPVLLGEAS
                     LATSMADRLLEEQVYVVGFSYPVVPEGQARIRVQMSAAHTHQQLEQAVAAFAKVGRDL
                     GVISKR"
     gene            complement(5370..6296)
                     /locus_tag="VFR82_02550"
     CDS             complement(5370..6296)
                     /locus_tag="VFR82_02550"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEU4500505.1"
                     /translation="MRISWPRVILSVLSLHVLLTGCVAVATTGQATESQSSITLSGPV
                     SLVLVADLRDVAVSDVSVAARQSQASDGGGDDTYDPFAKSGEGAEQEYDPWESLNTKV
                     FEFNRQVDRYVLKPVAQGYDFIMPDFVQVGISNMLYNFRFPQRFLNNVFQGKVKGAGI
                     EVGRFLINTAVGYAGFLDLAKEVDLVTPEEDFGQTLGSYGVKPGPYLIIPFLQPFTVR
                     DFTGYVADIFLNPINWLVAPIIEVEGVPSLIAHKNRTTTTFVQIGARVGEIVNERSRN
                     LQKFQGVEEATLDLYTAVRNAYLQSRARAIQE"
     gene            complement(6411..7175)
                     /locus_tag="VFR82_02555"
     CDS             complement(6411..7175)
                     /locus_tag="VFR82_02555"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU4500506.1"
                     /translation="MNILDQFFVFHPERWHEKDWRSASGLPLEDVWFKAADGATLFGW
                     YVEAPADRPVVIWCHGNAGNIVNRLDNLRLLFRQNLSVFLFDYRGYGKSQNVRPSEEG
                     LYQDAIGAYDYLVRGRMLKPERLVIFGRSLGAAVAADLASHKPAAGLILESAFPSIEA
                     VAKHYYGGLPLHWLLGAKFRLIDRLPALSLPKLIIHGDRDDIIPVELGRQVYEAAKQP
                     KMFHVINGAAHNNTYQVGGMRYFQTFSDFAGHAIRS"
     gene            complement(7429..7749)
                     /locus_tag="VFR82_02560"
     CDS             complement(7429..7749)
                     /locus_tag="VFR82_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500507.1"
                     /translation="MKAVGVLFGMCLFLTACVATPLKFPSAPIGPNEKALGKTEATST
                     GIMLFGFIPINQNTRFEDAMREAAAKAGGTRLTDVTISEQWFYAVVLDGFIFKVEGTA
                     VANK"
     gene            complement(7774..8100)
                     /locus_tag="VFR82_02565"
     CDS             complement(7774..8100)
                     /locus_tag="VFR82_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500508.1"
                     /translation="MRLVGMVLFAVLLIGTAACHSTHVQIPTAPIAAGEKSLGPTWGD
                     HVGLLLFGFIPIQQNDRFVEAYNEAVQKAGATRLTNVTISERWWWGYVINGYVFKVEG
                     TAVTNK"
     gene            8299..9651
                     /locus_tag="VFR82_02570"
     CDS             8299..9651
                     /locus_tag="VFR82_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEU4500509.1"
                     /translation="MNSLSSIVEPCTCCDVLVIGGGPAGSTISALLAEQGWNVHVLEK
                     DSHPRFHIGESLLPQSVPMLRRLGVLPEVEKIGIVKYGAELVSHRFGRSQMFHFAKAY
                     DDSQPYAFEVKRAEFDAILARNATQKGAQFHEGVKAQRVEFRPGRTSLVCAEDRTGHV
                     RTWEARFIVDASGRDTFLSGQLRGKHRSKKHGSAAVFSHFEGVTRLTGLDEGNITAAW
                     LDDGWCWMIPFKDGTMSVGVVCHPDYIKSRRTPLDQFLLDTLRQSPPIAKRMERARPL
                     TPAYAAANFSYRRDTMSGDGYLMIGDAFAFIDPVFSSGVHLALNSGTLGAGVVDAFLR
                     GSPEYSVRRKHFERMVRRGIDTFSWFIHRFNQPAFQALFVSPKRPPKIERAVLSLLAG
                     DVFAQSKMRFPLFLFKVVYYIVFMLNWKENWSVARRRKRGSRATVTEVTDYAVKSASA
                     "
     gene            9672..10715
                     /locus_tag="VFR82_02575"
     CDS             9672..10715
                     /locus_tag="VFR82_02575"
                     /inference="COORDINATES: protein motif:HMM:NF044415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500510.1"
                     /translation="MRHRQASSPASAQRVLEPTGHVKISYLEPDELVRQLTTSETYPL
                     AVVSFGLPLPFSLPCPVVHLDLPLLDRSRRCELWSCDQPVRVYQENGVSVAMSGSLLF
                     GSIAVPEDPDFGLDRTTEIAYRRLLQQLLESGFPHLWRIWNYFPGINQDQHGLERYRL
                     FCIGRHQALADTLPGFPASLPAGTAVGTQSGPLQIYFLAGAHPALHLGNPRQVNAYDY
                     PSLYGPRSPSFARASLYRSDSATQLFISGTASVVGHQSQHKGLADLQARETMTNLRAL
                     LDRAHCVPASLEREARLQSIFKVYVRNRDHLDAVRRVTDDPFFASSHLLYLQGDLCRK
                     ELLVEIEGLVTAD"
     gene            10775..11710
                     /locus_tag="VFR82_02580"
     CDS             10775..11710
                     /locus_tag="VFR82_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU4500511.1"
                     /translation="MQRTSVTTVGISDSFHARTCDFSPLKQLHFTIDCDWVPGSEKGL
                     EALLDCCDRYRMTATVFFTGRFAEAYPSLVRGCHERGHQLGTHGWAHGGLEEDEDFRT
                     AGYDQQRTWIRRATQAVEQAAGVRPVIFRAPNLWIGETTLRILEEEGYLYDSSVPARR
                     FDLGFGRVHYLKYFWAPTQPYRPSEADLGKPGASAITEIPPSAFLFPVNMATLRVLGL
                     QTVGKMIAWIGRASRHLVFYCHPSEFIHAKDQSFPKSMSKWNTRGMGPKNLCLLEALL
                     DFLSSRHFVPTPLIGSATTHLDVSRPVVIRARAFS"
     gene            11737..12147
                     /locus_tag="VFR82_02585"
     CDS             11737..12147
                     /locus_tag="VFR82_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500512.1"
                     /translation="MKRVRWATVAACVALVAVIGCRGGGQVYQVKDAPVQTASGKDLS
                     LEEIRKEIIAAGVAAGWQMAPTKSGEIIGTLNIRSHQAVVSIPYTTKTYSILYKDSSN
                     LKYDANAQTIHENYASWIQRLDGAIRSRLAAAGS"
     gene            complement(12170..>13002)
                     /locus_tag="VFR82_02590"
     CDS             complement(12170..>13002)
                     /locus_tag="VFR82_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4500513.1"
                     /translation="ALGPTEGSAIRGCERDRCWVVIPAYNEAATVREVAKRSRQQCAQ
                     VIVVDDGSTDNTAESLAGLDVMVLRNESNVGKAESLWRGFQRALGHGAVAIITLDADG
                     QHRPEEIPWFVAAVQDNPETLFVGARKREHRKISPGRYWANCMADFWIGWAAGVRIED
                     TQSGFRSYPARLLREVVLRHDRPRSFVYESEILIEAARRGFPCRNIPVSAMARAGQRP
                     SNFRPLADALRITKMVSWKLMKRGMYLSGLWCFLTGKSNATQTVCRPAQLARSSPIVP
                     "
BASE COUNT         2775 a         3823 c         3635 g         2769 t
ORIGIN      
        1 caccggtgga gaggcgtcga actcctcaag tggttgaaaa ttcaccggca gattaaacag
       61 ataatcggac acggagcgca ttttgacatg gtgatactcg acgatccaac tcccgtcctt
      121 cttcgccagc ttcatgggaa actgcaaatc ggtggcgacc cattggtaat attgttcgat
      181 gtgctcgccc cgcttggtcg tgacttcata cagagtcgtg ggatgtcctt cgcggatctc
      241 ggtcccgatt tcctgccgcg agacctctcc atcgagtcca gctgtgaccc gcagatcctg
      301 gtcggcgtcg tacggcagtg tcttgaaatg tttcatccga gataacagca gccagaccag
      361 atgcttatcc ttgcggacga tggtgacgtt gaccggtcca agcgaatggt gttcgattcg
      421 ccacatctga tctcgataat aaataaatgc tttgtgggtc cgaccgtcga tcttggtgat
      481 ttgatcggcg gtaaactcca gcgctgaagc ggtaggcgtg cctgatccca tccccaaagt
      541 caaggaccag acaagccaaa tccagcgtcg catcatcccc atgctaacat accgcttgcg
      601 accgcccatg catctgacct catcacgaat ggatggtcaa cacgccggcg ccgttaatgg
      661 cgccggactt gagcgcctgc aaggcgcggt tcgcctgagc aagaggaaat cgttgcgtgc
      721 ggggcctgat gggaatggcc gccgcctcac gcaggagatc gagtccgtcc tgacgggtat
      781 tggcagtcac gctgcgaatg acccgctctc caaagagatc acgatcatac tcaagcggtg
      841 gaatggtgct catatggatc cctgccagcg ccagggtccc gcctcgttcc agggcccgca
      901 aggcaggaat caccaattgt ccggctggag cgaacatgat tgaggcatgc aggcgaacgg
      961 gaggagcgtc tgaggcgcct ccgacccagg cggcgcccag atcccgagcc aacgcctgat
     1021 gactcggatt gagggaacag acatagacct cacatcccca atgtcgtgca agctggatgg
     1081 caatgtgtgc ggacgcacca aatccataca gaccaagccg ctggccgggt tgaatctggc
     1141 tcagacgcaa ggcgcgatag ccaataatgc ctgcgcaaag caacggagca gcttcttcat
     1201 cggaaaatgc cggaggtatc ggataggcaa aggctgccgg gagcaccgca tattccgcat
     1261 acccgccgtc gacctgatac ccggtgaatc gggcgctcag gcacaagttt tcccggccgc
     1321 ttcggcaata tccacattgg ccacaggtgc cctgtaacca ggcgacgcca actcgctctc
     1381 cgatagttcg ttcgttgaca ccggatccca gacggctgat gagtccaacc gcctgatgtc
     1441 cggggatgag ggggagcttg ggacgttcca actcgccctc gacgacatgc agatccgtcc
     1501 ggcagatacc gcagacggag acgcggacaa ggacttcacc ggagccaggc tccggcatgg
     1561 gcacatcgtc gagccggagt ggatcgccgg ctacatcccg cgggctgcca agaaccatcg
     1621 ccttcatcat tcgcagcgtg taccgaattc accgaggaga caagaccact gctcgccacc
     1681 atcaagcgcc ttgctccaaa ccatgctgaa atccttgcgg ttacttttcc ttccgcgttg
     1741 aacccgcccg catattccct gtaattctcg tgtcacgctt gattgccttc acgattccag
     1801 cgcccagacc cacctctata catccctggt agtcagtgaa ctgtcccgtc gtgcttgaag
     1861 caaacatgtg cgaaacaaga aatgtgatgg ggaatgttcc gatcctcatt gccagaaggc
     1921 aagttctgcc tggggcacgg agcagtcggt gagtaggaga agaattgccc gcaagccgga
     1981 aacgccggcg atcatactgt tacgggacat ctattttttc ggctgcgccg gtgcctcgag
     2041 tttcccattc gaagaattgc cgacagattg cacttcgcga gcgctggaac gggctcccgt
     2101 tctcatgcgg acatggacgt caaccggctc atcgcccacc tcctcgggca agggagggaa
     2161 cggctgagcg tttacgaccg catagatccc tgcctcgtcg atttcccgcg caccggagcc
     2221 cttttcaatt tgaatcaatt gtgcccgccc gttcggataa agcttgaagc gcacccggac
     2281 agtttcgctg ctgggactgc gccgaacccc tcgcacctgt cgcgcccagc tgcgactgac
     2341 gaggaggctg acctgttgcc aataggcctt gccctggccg ggatcgccga gcggcatcaa
     2401 atcttcttcg agcagggaac ccgtcgagac gggagcgacg ccgctcggtt gcagctcatc
     2461 gacgatcaaa tcaccggtcg acggctcctc aggacgcacg acggggggct cgctcgtgtc
     2521 actcgacggc tcctggtggt ctagcgtcac atagacgatg cgcttcatga agcgttccag
     2581 cttctccgga cgggccctgg cgaacgtcac ctggatgcgc gcggcctttt gatgaaccga
     2641 cgtgcggctc gacggcatgg ggtcgagggt gacctccgct ttgagtgcgg acgattctcc
     2701 gtccggttcg agcgtaataa gttggggctc gaacagaatg gattcgcaca acgcaacgat
     2761 cggcccggtg ccctgtgtca ccccgcccaa tgtaatcacc agcgcgacgg gtttgccagt
     2821 ctgcagatcg ccgctggcat cctgttggtt gagttcaaag cgggcccagc gactgcccga
     2881 acccgccgct gacgagggcg agatgggggt gaggaaagag ccgcacaata ccactccaat
     2941 cccgaatgct cctataagaa gcatgaactt tcgatgatgc ggcggcgcgc acataagcgg
     3001 gcatcctacc gaggcgacag cgctcgcgca acccgtaatt ctcctgatgt taggcaagct
     3061 gaggcgagtc ggatacaata gagccatgcg tgcgctgatg aaaaccgccc cacagccggg
     3121 gttgactgcg acgacctgtc ccgatccgtc accgggacca cacgaggcag ttattcgcgt
     3181 caaggccact tcgctctgcg gaacggacgt tcacatttac aattgggatc cctgggctca
     3241 tagccgcatc cacccgcccc gtatcattgg ccatgaaatg tgcggtgagg tcgtggaagt
     3301 cggctctgcc gtgatgctgg tgaaggtcgg cgactatgtg gccgcagagt cgcacatcac
     3361 gtgtgggttg tgttttcagt gccgtaccgg ccaggcgcat gtttgccgca attacaagat
     3421 tctcggtgtg gaccgcgacg gttcgtttgc ggattacgtc gtgttgccgg aaaacgtgct
     3481 ctggcacacg tcgcgagcca ttgcgccgga gtacgcctcg ctccaggagc cgttgggcaa
     3541 tgccgtcgat gccgccctgg tggaggatct gacaggccat acggtcctga tcacgggttg
     3601 cggacccact ggcctccttg ccgcggccgt cgcgcggacg tccggcgcag ccaccatcat
     3661 tgcctcggat atcagcgagt accgcttagg cttggccaag caggtcggcg ccgatcacgt
     3721 cttgaacgcc aaaaccgaga cggccgcgtc gctgcacgaa gccattgacg agatcaccga
     3781 cggcgagggc gtcgatgcct cccttgaaat gtccggcaat ccggcagcgc tacatctcgc
     3841 cttcgatacc gttaaaaacg gcggccgagt tacgcttttt ggcatcccca ccggcccgat
     3901 tacctgcgat cttgcaaacg acatcatctt taagggcatc cgcgtacacg ggatcaccgg
     3961 ccggcggctc tttggaacct ggtatcggct ggccgggctc ttcaaggcag gattgaacat
     4021 caagcccatc gtgactcaca cgttccccat gagcgatttt gcccaggggt tcgatctcat
     4081 caagacgggc caatgcggaa aggtcgtcct ctttccgtaa gcagctgacc tgcttcaacg
     4141 acacgtacgt gctggtgagc tcatggctga ctccaccctc aaacagatcg tcagcgagca
     4201 gttggcgagc atccgcgcca aaggcctcca taaatcggag cggcagcttt tgagcccgca
     4261 gaacgcagat attttcgtcg cccagggccg tgtcttgaac ctctgcgcca acaactacct
     4321 cgggctggcc aatcatccgc gaatcatcga ggcagtcatc aaaggcctgc gagatcacgg
     4381 ctacggcatg gcatcagtgc ggttcatctg cgggacgcag gacattcaca aagagctcga
     4441 acgtgcgatc agcacgtttc tcgggactga cgatgcgatc ctctacggat cctgcttcga
     4501 tgcgaacgga ggactattcg aggtactgct cgatgaacgc gacgccgtga tcagcgacgc
     4561 cctgaatcat gccagtgtga ttgacggcat ccgcctctgc aaagccaaac gatttcgcta
     4621 tgctcattcc gacatggagg atttagaagc gaggctgcac gagacccggc cataccgtct
     4681 gcgtctgatc gcgacagacg gcgtgttttc catggacggc gatctggcga gactcgatca
     4741 aatcgttgag ctcgcgcaac gtttcgacgc cgcggtaatg gtcgacgaca gtcacgccac
     4801 cggcgtgatc ggacgaaccg gccgagggac tccggatcac ttcggcgtcg ccgatcgtgt
     4861 cgacatcgtg acgagtacct tgggtaagac attgggcggc gcgaccggag gattcacggc
     4921 gggacacgcc ggggtgattg agctcctccg tcagcgttcg cgaccctatc tgttctcgaa
     4981 ttcgctgcct ccgccgatag tggccggggc cctgcaggcc ctcgccctgg ttgcggaagg
     5041 tgataacctg agagcacggc tgagtgagaa cgccgcgttc tttcgcgccc ggcttacgga
     5101 actgggtttc cggctcgtcc caggccacca ccccatcatt cctgtcctgt tgggagaggc
     5161 gtcgctggcc acctcgatgg cggatcgact actcgaggag caggtgtatg tcgtggggtt
     5221 cagttatccg gtcgtgccgg aaggtcaagc caggatccgc gtgcagatgt cggccgccca
     5281 tacgcaccaa caactggagc aagccgtggc cgccttcgcc aaggtaggtc gggatttagg
     5341 tgtgatttcg aagcggtgag gtttcgggat tattcctgaa tcgctctggc gcgggattgc
     5401 agatacgcgt tcctgacggc ggtgtagaga tcgagcgtcg cttcctcaac gccctggaat
     5461 ttctgcaggt ttcgtgaccg ttcgttgacg atctcaccga cacgggcgcc aatttgaaca
     5521 aacgtcgtcg tcgtacgatt tttatgcgcg atcaatgatg gtacgccctc gacttcgata
     5581 ataggcgcaa ccagccagtt gatcggattg agaaagatat cggcaacata gcccgtgaaa
     5641 tctctgaccg taaacggctg aaggaacggg atgatcagat agggtcccgg tttcacgcca
     5701 tacgagccta gcgtttgtcc gaaatcctct tccggcgtga cgagatcgac ttctttggct
     5761 agatccagga atccagcata ccccactgcg gtattgatca agaagcgtcc cacctcgatg
     5821 ccggcgcctt tgactttccc ttgaaacaca ttattcaaga accgctgcgg gaagcggaag
     5881 ttgtagagca tgttgctgat gccgacttgg acaaagtcgg gcatgatgaa atcatagccc
     5941 tgcgccaccg gtttcagcac gtagcgatct acctgacgat taaattcaaa taccttcgtg
     6001 ttcaaggatt cccagggatc gtactcttgc tcggctccct caccggattt cgcgaagggg
     6061 tcatacgtgt cgtccccacc gccgtccgat gcctgcgatt gcctggccgc gactgagacg
     6121 tccgacacgg cgacgtcccg gaggtcagcc accaggacta aagacaccgg cccactaagg
     6181 gtgatagagg attgactctc cgtcgcctgt cccgttgttg cgactgcgac gcagcccgtc
     6241 agaaggacgt gcaggcttag caccgagagg atcacgcggg gccacgagat cctcacattt
     6301 acgctccaga aacgaatgat aagggcaccg acggccggga ggctgcgggg tggcgctgca
     6361 tggtcgcgca ctgtaccaaa agttccacgc tcgcgaaacc attttttgtg ttacgagcgg
     6421 atcgcgtgac cggcgaagtc gctgaacgtc tggaagtacc gcattccacc gacctgatag
     6481 gtattgttat gggcggcgcc gttgatcaca tgaaacatct tgggttgctt cgcggcttca
     6541 tagacctggc gccccagttc aaccggaatg atatcgtccc gatcgccgtg aatgatcagt
     6601 ttcggaagag agagtgccgg gagtcgatcg attaatcgaa attttgcccc gaggagccaa
     6661 tggagcggta ggccgccata atagtgcttg gcaaccgctt cgatcgacgg aaatgcggat
     6721 tccaggatga ggcctgctgc gggtttgtgg ctggccagat ccgccgcaac ggccgcacca
     6781 agtgaacgtc cgaaaatgac gagtcgctca ggtttaagca tgcgcccacg gaccaggtaa
     6841 tcgtacgcac cgatcgcatc ctgatacagc ccttcttcgc tgggccggac attctggctt
     6901 ttcccgtacc cacgataatc gaacaagaag accgacagat tctgacgaaa aagcaaccgg
     6961 agattgtcga gccgattgac aatgtttccg gcgtttccat ggcaccaaat tacgacaggg
     7021 cggtccgcgg gcgcttcaac ataccacccg aagagtgtgg cgccatccgc cgccttaaac
     7081 cagacgtcct cgagcggaag gccgctggca gagcgccaat ccttctcatg ccagcgttca
     7141 ggatgaaaga cgaagaactg gtcaaggatg ttcatggcca tacgacttta atttatgcgc
     7201 gtcgtccccg gacacgggaa atatccaaga cgacatcgag atgttacagg tttttgactt
     7261 cccgagtacc taggaggatt ggaagggaaa agagtcccgt cgtcttgaga tcgacctgcg
     7321 aacggattgc cctttacctt attagaggaa gataagaaga gccgagacca gcgggctccg
     7381 acgcattcag accaagatac gccggagccg caacccggaa cgacgtgtct atttgttggc
     7441 gacggcagtg ccttccactt tgaagataaa tccatcgagc acgactgcat agaaccattg
     7501 ctccgagatc gtcacatccg tgagtctcgt cccgcctgct tttgcagccg cctcccgcat
     7561 ggcgtcttca aaccgcgtat tttggttgat aggaatgaat ccaaacagca tgattcccgt
     7621 gctggtagcc tcggtctttc ccagagcctt ttcattcgga ccgatcggtg cagacgggaa
     7681 tttcagagga gtcgcgacgc acgccgttaa gaagagacac attccgaata gtacaccaac
     7741 agctttcatg ggtttctccc tatgtgggta ggattacttg ttcgtgacag ccgtgccttc
     7801 taccttaaag acatatccgt tgatcacata accccaccac catcgttcgg agatcgtcac
     7861 attcgtcaac ctcgtcgcac cggctttttg cacggcctca ttgtaggctt cgacgaaccg
     7921 gtcgttttgt tgaatcggga tgaatccgaa cagcagcagg cccacatgat caccccatgt
     7981 tggtcccagc gatttctcac ccgccgcaat aggagcggtt ggaatttgca cgtgtgtcga
     8041 gtgacatgcc gcggtgccga taaggagtac ggcgaagagg accatgccca ccagtctcat
     8101 gtttccctcc ttgctacggt gaccgttccc aaggttgctc ttagtgagaa ttgcggcgcg
     8161 gagactacca cttaggacag aatcggtcaa cacgggtgct gaaagaagct tctgatccgt
     8221 ttgacgcccg ctagccaacc tcgcgtttct tgggtatact ccggcgacgg tttccgaagt
     8281 ttgccaatat ctttccagat gaacagcctc tcttctatcg ttgaaccctg cacatgttgt
     8341 gacgtgttgg tgatcggagg tggaccggca ggctccacaa tttcggcgtt gctcgcggaa
     8401 cagggctgga acgttcacgt cctggaaaag gactcccatc cccgattcca tatcggggag
     8461 tctttgcttc ctcaatcagt tcccatgctt cgacggctcg gggtgttgcc tgaggtggaa
     8521 aagatcggca tcgtcaagta cggtgccgaa ctggtatcac atcgctttgg ccggtcgcag
     8581 atgttccatt ttgcaaaggc atacgatgac tcccagccct atgccttcga agtgaaacgg
     8641 gccgaattcg atgccatcct ggcccgcaac gcgacgcaaa aaggtgcgca gtttcatgaa
     8701 ggggtaaaag cccaacgggt cgaattccgc cctgggcgga cgtcattggt ctgcgcagaa
     8761 gatcgaacgg ggcatgttcg aacgtgggaa gcccggttca tcgttgatgc aagcgggcgt
     8821 gacacatttt tgtccggaca attaagaggc aagcaccgca gcaagaagca cggcagcgcc
     8881 gcagtcttca gtcatttcga aggagtcacg agactgaccg gattggacga aggaaacatc
     8941 acggctgcct ggctcgacga tggctggtgc tggatgattc cgttcaaaga tggaacgatg
     9001 agtgtaggcg tggtgtgcca ccccgattac attaaatcca ggaggactcc gctcgatcaa
     9061 tttctcctgg acactttacg acaatccccc cccattgcca aacgtatgga acgagccagg
     9121 cctttgacgc ccgcatacgc tgcagccaat ttctcctacc gtcgtgacac gatgtcggga
     9181 gatggatatt tgatgatcgg ggacgccttt gcctttatcg atccggtgtt ctccagcggt
     9241 gttcatctcg cgctgaacag cgggaccctg ggcgcagggg tcgtcgatgc atttctgcga
     9301 ggctcgccag agtattccgt ccggcggaag cattttgagc ggatggtgag acgaggcatc
     9361 gacacctttt cctggttcat tcacagattc aaccaacctg cgtttcaagc gctgttcgtg
     9421 tcgcctaagc gtcccccgaa gattgaacgg gccgtcctgt cgttactggc tggagacgtc
     9481 ttcgctcaat cgaagatgcg cttccctctt ttccttttta aagttgtcta ttacatcgtt
     9541 ttcatgttga attggaaaga aaactggagc gtggcccgca ggcgtaagcg tggcagccgc
     9601 gccacggtaa ccgaagtgac cgactacgcc gtgaagtcgg cgtcagcata gggtcaatcc
     9661 gataaatggg catgcgacac cgtcaggcgt cctctcccgc gtccgcgcag cgggttctcg
     9721 aacccacggg tcacgtcaaa atcagttacc ttgagccgga tgagcttgtc cggcaattga
     9781 cgacatcgga gacctacccg cttgccgtcg tttcgttcgg tctgcctctg ccgttttccc
     9841 tgccctgtcc ggtcgttcac cttgatctcc cgctgttgga tcgatcccgc cgatgcgaac
     9901 tttggagctg cgatcagccg gtccgcgtgt accaggagaa cggggtttcg gtagccatga
     9961 gcggctcttt gctgttcggc tccattgccg tgccggaaga tccggacttt ggtctcgatc
    10021 ggaccactga aattgcctac cgtcgcctcc ttcagcaact gctcgaatca ggattcccac
    10081 atttatggcg gatctggaat tattttccgg gtatcaatca agaccagcat ggcctggaac
    10141 ggtaccggtt gttctgcatc ggacggcacc aagccttggc cgacacattg ccggggtttc
    10201 ctgcctcact gccggccggt acggctgtgg gaacccaaag cggcccactt cagatttatt
    10261 tcctcgccgg cgcccatcca gccttgcatc ttggcaaccc acgtcaagtg aatgcgtacg
    10321 actatccaag cctctatggc cctcgcagcc cctcgttcgc tcgtgccagt ctgtaccgct
    10381 cggacagcgc cacgcagttg ttcatctccg gtacggcgag cgttgtcggg catcagagcc
    10441 agcacaaggg tttagccgac ctccaagctc gagaaacgat gacgaacttg cgggcgctgc
    10501 tcgaccgtgc ccattgcgtt ccggccagcc ttgagcgaga agcacggtta caatcgatct
    10561 tcaaagtata cgtgcgaaat cgagatcatc tcgatgccgt acgtcgggtc acggacgatc
    10621 cgttttttgc ttcaagccac ctgctctatc ttcaaggcga tctctgccgc aaggaattgt
    10681 tggtcgaaat cgaagggctc gtcactgcgg actgatgcct ttccccttac tctgcacgcc
    10741 atacggaaac cggttgaaca ccaaaatgaa cctcatgcag cgaacatcag taactaccgt
    10801 cggaatctcc gactcattcc atgcgagaac atgcgatttt tcacccctga aacagttgca
    10861 cttcaccatt gattgcgatt gggtgccggg atcggagaaa ggcctcgaag ctctcctcga
    10921 ttgttgcgac cgttatagaa tgacggcgac cgtctttttc acagggcgtt ttgccgaggc
    10981 gtatcccagc ctggtgaggg gttgtcacga gcgcggacat caattgggga cgcatggatg
    11041 ggctcatggc ggccttgaag aggacgaaga ctttcgaacc gccgggtacg atcagcaacg
    11101 aacctggata cggcgggcca ctcaagccgt tgagcaggct gccggtgttc gccccgtcat
    11161 tttccgcgcc ccgaatcttt ggatcggtga aaccaccttg cggatccttg aagaagaagg
    11221 ctatctctac gattcttcgg tcccggccag gcgctttgac ttgggattcg gacgtgtcca
    11281 ctatctcaag tacttctggg ctccgaccca gccttatcgg ccatctgaag cggacctggg
    11341 caaaccgggc gccagtgcca tcacagaaat accgccgtct gcctttctgt tccctgtcaa
    11401 tatggcaacg ctccgagtgc tgggtttgca gacagtcggt aaaatgatcg cctggatcgg
    11461 gcgggcatcc cggcacctgg tcttttactg ccatccatcg gaattcatcc acgcgaaaga
    11521 ccaatcgttt ccgaaaagca tgtcgaaatg gaacaccagg gggatgggtc ccaaaaactt
    11581 gtgcttgctc gaagcgctgc tggattttct ctcttccaga cactttgtgc ccacccccct
    11641 tatcggcagc gcgaccaccc atcttgacgt gagccgtcca gtcgtaataa gagctagagc
    11701 gttctcctga gccgttcttt caatgggagg tcagccatga agagagtaag atgggcgaca
    11761 gtagccgcct gtgttgcgtt ggtagccgtg atcggctgca gaggcggggg gcaggtttat
    11821 caagtcaaag atgcacccgt gcaaactgca tcaggaaaag acctgagcct ggaagagatt
    11881 cgaaaagaga ttattgcggc aggagtcgca gccggctggc aaatggcgcc taccaaatca
    11941 ggcgaaatta ttgggactct caacattcgc agccatcaag ccgtcgtgtc tattccctat
    12001 accaccaaga cctacagcat tctctacaag gacagctcga acctcaagta tgacgcgaac
    12061 gcgcaaacca tacacgagaa ttatgccagt tggatccaac gattggatgg cgccatccgc
    12121 tcgcgcttgg ccgctgcagg aagttaactc ctcacgggca caacagacat catgggacga
    12181 tcggcgagga ccttgctagc tgagccggcc ggcagactgt ttgcgtggca ttcgacttgc
    12241 ccgtgagaaa gcaccatagt ccgctcagat acatccctcg cttcatcagc ttccatgaca
    12301 ccatttttgt gatccgaagc gcgtccgcga gcggtcggaa attgctgggg cgctgaccgg
    12361 cgcgcgccat cgccgacacc ggaatattcc ggcacggaaa tccgcgtctt gctgcctcga
    12421 tcaggatttc actttcatag acaaagctcc gagggcggtc atgtcggagc actacctcac
    12481 gtagcaagcg ggccggatac gaacggaacc ccgactgcgt atcctcaatc cgcacgcctg
    12541 ccgcccagcc gatccagaaa tcagccatgc agttggccca ataacgccca ggagatatct
    12601 tccgatgctc gcgtttccgc gcaccgacaa agagcgtctc aggattgtcc tgcactgccg
    12661 caacaaacca gggaatctcc tccggcctgt gctgcccatc tgcatcgagc gtaatgatag
    12721 cgaccgctcc atgacccaat gctcgctgga atccgcgcca aagactttct gccttcccga
    12781 cattactctc atttcgtaga accatcacgt ccagacctgc caagctctcg gcggtgttgt
    12841 cggttgagcc gtcgtccacc acaatgacct gtgcacattg ttggcgcgat cgcttagcca
    12901 cttcccgtac cgtcgcggct tcgttgtatg ccggaatgac gacccaacag cgatcgcgtt
    12961 cgcagccgcg tatggcgctg ccttctgtcg gtcccagggc ga
//
LOCUS       DASTQY010000081         7185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1357387, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000081 DASTQY010000000
VERSION     DASTQY010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357387"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFR82_02595"
     CDS             complement(<1..258)
                     /locus_tag="VFR82_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU4500514.1"
                     /translation="MMPPFLHLHVHSQYSPMRGVSSLAALCRSARQYGFPAMALTDTN
                     GLYGAIRFIEEAKQQGLRPILGAELTADNHRAVLLAKTPAGY"
     gene            complement(255..1460)
                     /locus_tag="VFR82_02600"
     CDS             complement(255..1460)
                     /locus_tag="VFR82_02600"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500515.1"
                     /translation="MALTAWLWMMDRQIVYLDIPSFGIALARAQNTALRNRPVAMAPV
                     HTPRALIREVSVEASSQGLQPGMSVELARLLCPDLRLVAPDLSHAQAAHRELQQRVLS
                     FAPVWESIRPGSLFLDLTGTTRLLGAPIDIATRIGRELTRYHRWHSVIGLAGNKLVSQ
                     LAATTLHRPPQVLWIQRGSEQPFLAPLPSIWLPGLPRTRASKLVQRLEDLNLRTLGAI
                     ASVSVAQLEAAFGASAALLHDWALGVDPSPVRPPAAQPMIEWAISLDPDEVDDHLLLG
                     RLYGLLERICTTLRHQQRICRFLRLSVRHSDHVEREAHERLAHGTYWEADLQPLLTRL
                     FYRCMRRRVRLTRLTLQAGKLEPPAEQLSLFDAWTSAPPASHRLSRALDAIRAKFGEQ
                     SLSWGRTLR"
     gene            1634..1981
                     /gene="rpsF"
                     /locus_tag="VFR82_02605"
     CDS             1634..1981
                     /gene="rpsF"
                     /locus_tag="VFR82_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU4500516.1"
                     /translation="MELYESLFIIRPSVSDEETNALIEKMKGVADRTGAQFIKSENWG
                     RKKLAYEVRRERKGTYVYFYFKAPNNTVGELEHAYRLEDNIIKFLTVHLEKELVPRRV
                     AETPSEEFTGGRV"
     gene            1968..2378
                     /locus_tag="VFR82_02610"
     CDS             1968..2378
                     /locus_tag="VFR82_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU4500517.1"
                     /translation="MAGFNKVILIGNLTRNPELRYTPNGTPVAALGLAVSRRYKQGDD
                     LKEEVCFVDIVVFGKQAEHCGQYLSKGNGIIVDGRLQQRRWETEDGQKRSKHEVVAQT
                     VTFLPKRGDGGGQNAGGESGMHENSNYEYEEPQG"
     gene            2387..2659
                     /gene="rpsR"
                     /locus_tag="VFR82_02615"
     CDS             2387..2659
                     /gene="rpsR"
                     /locus_tag="VFR82_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU4500518.1"
                     /translation="MERERGGSGGGRGDGGGGGRFFQRRRPCRFCIEKGSIDFKDVGL
                     LRNFLTERGRIVPRRISGNCLGHQRELTTAVKRARHIALISFAEER"
     gene            2699..3286
                     /locus_tag="VFR82_02620"
     CDS             2699..3286
                     /locus_tag="VFR82_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU4500519.1"
                     /translation="MGVLTPLVADISTEGLSLVGEATAEELELTAADATLRGPVSVSL
                     DMMQADDMIAVTGVLEGTAVRQCVRCLKEYEDPLAFSVHAAFAREGKEFKPGVRPLKG
                     AEPRKGRPTLGKPEADAEPEDEGDDRYFYQGDHIELAPMLREHIILASPMQPLCRDNC
                     AGLCARCGKDLNEGPCQCPTEAPPSAIRVIRRTKN"
     gene            3342..3527
                     /gene="rpmF"
                     /locus_tag="VFR82_02625"
     CDS             3342..3527
                     /gene="rpmF"
                     /locus_tag="VFR82_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU4500520.1"
                     /translation="MPNPKHKHSRARRDKRRTQKLRVTPPGMSVCPQCHELKLPHYTC
                     LNCGTYKGKAVIQVEEV"
     gene            3737..4780
                     /gene="plsX"
                     /locus_tag="VFR82_02630"
     CDS             3737..4780
                     /gene="plsX"
                     /locus_tag="VFR82_02630"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU4500521.1"
                     /translation="MTTSSRMKIALDAMGGDHGPVPCIEGALQAAQELNVDVILVGDE
                     KVLSDACHRLGCTDGRLSIKHAPQVVEMHESPAAVARKKRDSSIWIATELVKSGEAQA
                     VVSPGNTGASMVASFFLLGLTKGVERPAIATSLPTLTGEAIMLDVGANVDCTAQHLIQ
                     FAIMGNEYGQHLFKKPNPRIGLLSIGEEDSKGNEVTKEAFKLLRNSSLNFIGNVEGRD
                     VYSGNADIVVCDGFIGNVALKISEGVADTIKKLLFKEISGSFFGRLAYPLIATPLVKL
                     KRRIDYAEFGGAPLLGVNGITIICHGRSSAKAIKNAIRRAKGLAESHVDQLIQRDIEE
                     SRSQSRPAEEPSS"
     gene            4777..5751
                     /locus_tag="VFR82_02635"
     CDS             4777..5751
                     /locus_tag="VFR82_02635"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEU4500522.1"
                     /translation="MRAHITGTGSYVPSKILSNADLENMVATSDQWITERTGIRARSV
                     CGPGEACSDLGVKAAQRALEDAKISASDLDMILLATCTGDYPLPATACLIQQQLGAKR
                     AAACDLSAACCGFVYALSIADAYIRTGMRHVLVIGSEVMSAITDWTDRNTCVLFGDGA
                     GAAVVSATEGDRGILSTHLRSEGELWELIAVPGGGSRLPPCEKVLAEKLNCIKMKGNE
                     TFKVAVRTLEEIARETLAANRMTISDLDLYVPHQANVRILKAVADRLGLPLEKVMLNL
                     DRYGNTSAASIPIALDEAIRQGRITKGSLVMLGAFGAGLTWASALIRW"
     gene            5877..6815
                     /gene="fabD"
                     /locus_tag="VFR82_02640"
     CDS             5877..6815
                     /gene="fabD"
                     /locus_tag="VFR82_02640"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU4500523.1"
                     /translation="MNPAVGFLFPGQGSQSVGMGKALFEAHSELKQIYAEATEVLGYD
                     IAELCFNGPAETLNLTEHTQPALLVSSVAALRVLEPAGIIPLAVAGHSLGEYTALVAA
                     GGLTYRDAVAIVQKRGRYMAEAVPAGTGLVAALLGLATDAVKEACREAASVGIVQAAN
                     FNSPGQVVIAGEKAAVERAIELAKGKGCKKAVPLPVSVPVHTPLMQKAADRLAKELAA
                     ITWSDLRMPLINNAEARPVTKAGDIPASLVRQLPSSVLWEDSVTRMASMGIKTFVEVG
                     PGTVLTGLVRRIVPDAVTLNVNDPKSLAATRMALRQ"
     gene            6833..>7185
                     /locus_tag="VFR82_02645"
     CDS             6833..>7185
                     /locus_tag="VFR82_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4500524.1"
                     /translation="MSLQGRVAIVTGAAQGIGRAIAETLAEAGADVVVADLDPVRSQD
                     AVAAIEKIGRKALNVKVNVADSNDTKTMADHVLKQWGKIDILVNNAGITRDGLLLRMK
                     EEDWNLVLQVNLNGT"
BASE COUNT         1608 a         1936 c         2244 g         1397 t
ORIGIN      
        1 ataaccggcc ggcgttttgg ccagcaggac cgcccgatgg ttgtcggccg tcaactctgc
       61 cccgagaatg ggccgcagac cctgctgctt cgcttcctcg ataaaccgga tggcgccgta
      121 caatccgttg gtatccgtga gagccatggc ggggaatccg tactggcgag ccgagcggca
      181 gagtgccgcc agcgacgaca ccccgcgcat cggcgaatac tgagaatgaa catgcaggtg
      241 gaggaaaggg ggcatcatcg cagagttctc ccccaggaca gggactgctc gccgaatttc
      301 gcgcgaatcg catcgagtgc cctagacagc cgatgggatg ccggcggggc cgaggtccac
      361 gcgtcaaaga gcgacagttg ctcggccggc ggctccagtt ttcccgcctg cagcgtcagt
      421 cgtgtgaggc gcactcgccg gcgcatacac cggtaaaaca ggcgcgtgag cagcggctga
      481 agatcggcct cccaatatgt gccatgtgcc aatcgttcgt gcgcctctcg ctcaacgtga
      541 tcgctgtggc ggacagagag acggagaaag cggcagatcc gctgttggtg gcgaagggtg
      601 gtgcaaatcc gttccagcaa accgtacagc cgtccaagga ggaggtgatc gtccacttca
      661 tcgggatcga gagagatggc ccattcgatc atcggctgtg cggcaggagg gcggaccggc
      721 gatggatcaa cccccaaggc ccagtcatgc agcagagcgg cggaggcgcc gaacgcggct
      781 tcgagctgcg ccaccgagac cgaggcgatc gcgcccagtg tgcgaaggtt caaatcttcc
      841 agccgttgca cgagctttga cgcgcgggtt cgagggaggc cgggaagcca tatggacggc
      901 aaaggagcga gaaacggttg ttcggacccg cgctgaatcc ataagacttg aggcggtcgg
      961 tgcagtgtcg tcgccgccag ctgtgagacc agcttgtttc cagccaatcc gatcacgctg
     1021 tgccatcgat ggtatcgggt taattcacgt ccgatgcgcg tggcgatatc gataggcgca
     1081 cccaaaagcc gggtcgtgcc ggtcaagtcg agaaacagcg agccaggccg gatcgattcc
     1141 cagaccggtg caaaggacag gacacgctgt tgcaactcgc ggtgcgcggc ctgcgcatgc
     1201 gataaatctg gagcgaccaa ccgtagatcg ggacaaagca gacgcgccaa ttcgacggac
     1261 atgcccggct ggagtccttg agaagaggct tcaacagaaa cttctcgaat caaggcccgc
     1321 ggtgtatgga ctggcgccat ggccaccggt ctgttccgca acgcagtgtt ttgggcacgg
     1381 gcgagcgcaa tcccaaacga aggaatgtct aaatatacga tctgccgatc catcatccaa
     1441 agccatgcgg tcaaagccat gtggtgtaac caatggttac aaacacatga gagaaagtca
     1501 accagatgaa gaaccctatt tgcgccggca ttgtcggcta tgttagtttc tccgcttcgc
     1561 gtcggctgaa aggccgagca cctacacctc gctccggacg cgttccgggg cctttgtcca
     1621 ggaggaactc tgcatggagc tctacgagtc tctattcatt attcgtccgt ccgtctccga
     1681 tgaggagact aatgcgctta tcgagaagat gaagggtgtc gccgatagga ctggcgcgca
     1741 attcatcaag tcagagaact ggggcagaaa gaagctggcc tatgaagtcc ggcgtgagcg
     1801 gaagggtacg tacgtctact tctacttcaa ggcgccgaac aatacggtcg gcgaattgga
     1861 gcatgcctat cgcctagagg acaacatcat caagttcctc accgtccatc tcgaaaagga
     1921 actggtgccc cgccgggtcg cggagacacc atctgaggag tttaccggtg gccgggttta
     1981 ataaagtcat tcttatcggg aatctgaccc gcaaccccga gttgcggtat acgccgaacg
     2041 gcacgccggt cgccgcactg ggcttggcag tgagccggcg ctataagcag ggtgacgacc
     2101 tgaaagaaga agtctgtttt gtggacatcg ttgtgttcgg gaaacaagcc gaacactgtg
     2161 gacagtacct cagcaaaggg aacggcatca tcgtggatgg ccgattgcag caacggcgtt
     2221 gggaaacgga agacggtcag aagcgcagca agcatgaagt ggtcgctcag acggtcacat
     2281 ttctaccgaa gcgcggtgac ggcggcggcc agaatgcggg tggcgagtcc ggcatgcatg
     2341 aaaattccaa ctatgagtac gaagaacccc aaggatgagg gacaacatgg aacgtgaacg
     2401 cggcggcagc ggcggagggc gaggcgatgg gggcggaggc gggcggtttt ttcaacggcg
     2461 ccggccatgc cggttttgta tcgaaaaggg ctcaatagat tttaaggatg tcggcctcct
     2521 gaggaatttt ttgacggagc gggggcgcat cgttccccgc cgaatctccg gcaactgtct
     2581 gggacatcag cgcgaattga cgaccgccgt caagcgggcc cggcatatcg cactgatcag
     2641 ttttgccgaa gagcggtaaa agacggtctg aggaatggac atccaagcag gtatgacgat
     2701 gggagtgctc acaccgctgg tagccgatat ttcgacagaa gggctgtcac tggtcggcga
     2761 agcgacggct gaagaactcg agttgacggc ggccgatgcg acccttcggg gtccggtgtc
     2821 cgtaagtctg gacatgatgc aggctgacga catgattgcg gtgaccggtg tgctggaagg
     2881 gacagcggtc cgacaatgtg ttcgatgcct gaaggaatat gaggacccat tggcattttc
     2941 cgtccatgcc gcatttgctc gtgagggcaa ggaattcaaa cctggcgtgc gtccactgaa
     3001 gggcgccgag ccccgcaagg gccggccaac gctgggcaag ccggaagcgg atgcagagcc
     3061 agaagacgaa ggagatgatc gctatttcta tcagggcgat cacattgaac tggcacccat
     3121 gttgcgggag cacattattt tagcttctcc gatgcagccg ctctgccggg acaattgcgc
     3181 cgggctgtgt gcgcgatgcg ggaaagattt gaacgagggg ccttgccagt gtcccactga
     3241 agcgccaccg tcggcgatcc gggtgattcg aagaacaaaa aactgatcag ttggtcacag
     3301 cgagggagcg ccgctggaga ccggttgctg gaggaaagac gatgcccaat cccaaacata
     3361 agcattcgag agccagacgc gacaagcgcc ggacacagaa actacgggtc acgcctcccg
     3421 gtatgtccgt atgcccgcag tgtcacgaac tcaagctgcc tcactacacg tgcttgaact
     3481 gcgggaccta caaggggaag gccgtcatcc aggtcgaaga agtgtaaggg ccagacgtaa
     3541 agcttccggc gaagacacga cctgtgctgc tatcttggaa gggccgctgc tcgcatagtt
     3601 gagcagtgat tcgcgcccga tgtgttgccg caagtattac gcgtccccaa tcggttgcgt
     3661 cgttgggcat actgctctac actcgcctcg tactcgaact cttcagtagt gttttcttat
     3721 gggtaagcgg accgtcatga ccacatcttc gcgcatgaaa atcgcgcttg atgccatggg
     3781 tggagaccat ggtcctgtcc cctgtatcga gggcgccctt caagccgctc aggaactgaa
     3841 cgtcgacgtc attctcgtcg gtgacgaaaa ggtgctttca gacgcctgtc accggcttgg
     3901 ttgcaccgat ggccgtctgt ccatcaagca tgcaccgcag gttgtagaga tgcatgaatc
     3961 tcctgcagcg gtggcgcgca agaagcggga ttcgtccatt tggatcgcca ctgagttggt
     4021 gaagagcggt gaagcccagg cggtggtgag tcccgggaat accggcgcca gcatggtggc
     4081 atcctttttt cttctcgggt tgaccaaagg cgttgaacga ccggccatcg cgacaagtct
     4141 acctacattg actggagaag cgatcatgtt ggacgttggc gccaacgtcg attgcaccgc
     4201 gcaacatctc attcaattcg cgatcatggg gaacgaatat gggcagcatc tttttaaaaa
     4261 gcccaatcca cggatcgggc tgttgagcat cggcgaagag gatagtaaag gcaacgaagt
     4321 gacgaaagaa gcattcaagc tgttgcggaa cagctcattg aatttcatag gcaacgtcga
     4381 agggcgtgac gtctacagcg gcaatgcgga cattgtcgtc tgcgatggct tcatcgggaa
     4441 cgtcgccctg aagatttccg aaggagtcgc tgataccatc aagaaactgc tcttcaagga
     4501 aatatcaggg tcgtttttcg gacggttagc ttatccgttg atcgccactc ctttagtgaa
     4561 actgaaacgc cggatcgatt acgccgagtt cggcggcgct ccgctactcg gggtgaatgg
     4621 gattacgatc atctgtcatg ggcgttcgtc agccaaggcg attaaaaatg ccattcgccg
     4681 cgcgaagggc ctcgccgaga gccatgtcga ccaattaatt caacgggaca tcgaggaaag
     4741 ccggtcccag agccggccgg ctgaggaacc ttcgtcatga gggcgcacat cacgggaacc
     4801 gggtcctatg taccctcaaa aatcctctcc aatgcggact tggagaatat ggtggcgacg
     4861 tccgaccaat ggatcactga gcgtacgggc attcgggccc gttctgtgtg cggtcccgga
     4921 gaagcgtgtt cggatttggg agtcaaggca gcacagcgcg cgctcgagga cgctaagatc
     4981 agcgcctccg acctcgacat gatcctgctg gccacctgta ccggcgatta tccgcttccg
     5041 gccaccgcct gtttgattca acagcaattg ggcgccaagc gagccgcagc ctgcgatctc
     5101 tctgcggcgt gttgcggatt tgtgtacgcg ctgtcgatcg cggacgccta catccggacc
     5161 ggcatgcgcc atgtcttggt gatcggatcg gaagtcatgt cggcaatcac cgattggacg
     5221 gaccggaata cctgcgtcct cttcggggac ggcgcaggag ccgcggtggt cagcgccact
     5281 gaaggggatc gtggcatcct ctctacacat ttacgatccg agggtgagct ttgggaattg
     5341 atcgccgtgc cgggcggcgg ctcacgattg ccgccctgcg agaaggtgct tgctgagaag
     5401 ctgaactgca ttaagatgaa aggcaatgag accttcaagg tggccgtgcg tacgttggag
     5461 gaaatcgcgc gagagacgtt ggccgcgaac cgtatgacca tttcggacct ggatctctat
     5521 gtcccgcacc aggccaacgt tcggatcctg aaggcggtgg cagaccggtt gggtttgccg
     5581 cttgagaagg ttatgctgaa ccttgatcgc tacggcaaca cgtccgccgc gtcaataccc
     5641 atcgctcttg acgaggccat aaggcagggc cggatcacca agggctccct cgtgatgttg
     5701 ggcgcctttg gggccgggtt gacttgggct tcagcactga tccggtggta aatctgtaac
     5761 cgcaacagac cgtggttgcc cgcaccgtga agcagcacga tccggtgtgt ggcggaggcc
     5821 tccagggaac ttttcccgtt gactttccag cgcgtttcga agatatctaa ccgcttatga
     5881 atccagcagt cggcttctta tttcctggcc aaggttctca gtcggtagga atgggaaagg
     5941 cgcttttcga ggcgcattca gagctcaagc agatctatgc cgaagccacg gaggtgctgg
     6001 gatacgatat tgccgagctt tgcttcaatg ggcctgcgga aaccttgaac ctgaccgagc
     6061 atactcaacc ggcattgctc gtcagcagtg tggccgcgct ccgggtgttg gagccagctg
     6121 gaatcatacc actggctgtc gccggtcata gtctcggtga atacacggca ctcgtggcag
     6181 cggggggcct gacgtatcgc gacgccgtcg ccattgtaca aaagcgagga cgatacatgg
     6241 cggaagcggt gcctgctggc accggtttag tggctgctct ccttgggctt gcgaccgatg
     6301 cggtcaagga agcgtgccgg gaagcagcat ccgtgggaat tgttcaagcg gcgaacttca
     6361 actctccggg ccaggtggtc attgccggag aaaaggccgc ggtggaacgc gcgatcgagc
     6421 ttgccaaagg taaagggtgc aagaaagcag tgccattgcc cgtgagcgtt cccgttcata
     6481 caccgctgat gcagaaggct gccgaccggt tggcgaaaga actcgcagcg attacctggt
     6541 ccgatttgcg catgccattg atcaataacg cagaagccag gccagttacg aaagccggag
     6601 atattccggc atccttggtg cggcaactgc cctcatccgt gctctgggaa gactccgtga
     6661 cgcgcatggc gagcatgggc atcaagacct tcgtcgaagt ggggccaggt actgtcctga
     6721 ctggactggt gcgaagaatc gtacctgacg ccgtcaccct gaatgtgaat gacccgaaat
     6781 cgctggcggc cacgcgcatg gcattgcggc aatagaacaa ggagcagcag gtatgtcact
     6841 acagggtcgc gtcgcaatcg tcacaggagc ggcacagggc ataggccggg ccatagcgga
     6901 gacgctggca gaggcgggag cggacgtcgt cgtcgccgat ctcgatccgg tgcgctctca
     6961 ggatgcggtt gccgcaatcg aaaagatcgg caggaaagcc ctcaatgtca aagtgaacgt
     7021 ggccgactct aatgacacca agaccatggc agaccacgtg ctcaaacagt gggggaaaat
     7081 tgatatcctg gtcaataatg ccggtatcac ccgcgacggg ctgctactac ggatgaagga
     7141 agaagattgg aatctggtgc ttcaggtcaa tttgaacggc acatt
//
LOCUS       DASTQY010000082         3405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1354386, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000082 DASTQY010000000
VERSION     DASTQY010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354386"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VFR82_02650"
     CDS             complement(<1..351)
                     /locus_tag="VFR82_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500525.1"
                     /translation="MPTTTVTTPSASDILFHRAGSVTSTGPVKHQPEAPRMVRLTVNL
                     PSHLVEQMRDAVYWTPGLTLAWLVARAVRASLTELETIHQGPFAKRTKPLRAGRPRLL
                     GQSMKVRPLGGDDSI"
     gene            complement(802..1311)
                     /locus_tag="VFR82_02655"
     CDS             complement(802..1311)
                     /locus_tag="VFR82_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500526.1"
                     /translation="MDAFQSIVTDLNTLIPIVNHWFHLLSAVIWIGGLAFLVMAVTPG
                     LQKAVAREQIKPITDVFYQHYKKVAGILLVILLFTGGINLHYVNQVLTSQTGSGVQHH
                     AKYLTIFFIKLFLVLCLLTLFLYTVIFKSDDDAEEGESYEVIPFQRAALWMGFFIILC
                     AAAMKHLHQ"
     gene            complement(1408..2793)
                     /locus_tag="VFR82_02660"
     CDS             complement(1408..2793)
                     /locus_tag="VFR82_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU4500527.1"
                     /translation="MGKSLPIFNGPSLSGAIGSLQQQLTQFLTPKPQTEGPFDGRTVD
                     DFKPYLVALNLTKRCNLKCDHCYLDATTKAAGGDDELSTEECFRLIDQIAEVNKGCLL
                     VITGGEPLVRPDILEIARHAVKLGFMVVFGTNGMLIDDRMAKELVDIGVMGVGISIDS
                     LDSMKHNAFRGVPGAWEAAVAGIEASKRNGLQFQVHFSAQPMNYQELPAVVEWAHGLG
                     ARVLNVFFMVCTGRGEELTDITPAQYEEVLGYLVKCQDEYQGMLIRARCAPHFKRLAY
                     EKDPNSPITKATGYMGGGCLAGTNYARVTPNGELTPCPYMPLSAGNVRENSFVELWEK
                     SDIFNSFRYPQLKGKCGDCEYSDICGGCRARPYVDHGDWLDEDQWCLYTPKGGEKIKV
                     AFNVEESSTVQWDNAASLRLSRIPYFLRAMVKKGVEKHAREHGIETVTIELMEELRKK
                     RFGNDAPVFKF"
     gene            complement(2888..>3405)
                     /locus_tag="VFR82_02665"
     CDS             complement(2888..>3405)
                     /locus_tag="VFR82_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HEU4500528.1"
                     /translation="AKEAIRAVPAGFQRRRAKAKIEKSARKLGMTTITLEYAAPMIQE
                     AASEDYQPIFANKGAGTSAAAEAKLAQANGNGMRDVASPENGNGGAEASAYNWTAEAQ
                     ARLERAPEGFMRDCTKALIIKHAEKIGTTHISLEVANEGIEQAKGYMEEAMKTGNLKD
                     MIANLTGKGRA"
BASE COUNT          712 a          953 c          970 g          770 t
ORIGIN      
        1 tatagagtca tcgcctccaa gcggccggac tttcatggat tggcccaaca gtctgggccg
       61 gccggcccgg agcggctttg tacgtttggc aaaaggtcct tgatgaatgg tctccagttc
      121 cgtcaatgag gcccgcactg cccttgccac gagccaagcg agggtaagtc ccggcgtcca
      181 gtacaccgcg tcgcgcattt gttcgactag gtgcgacggc agattgacgg tcaaccgaac
      241 catgcgtggc gcttccggct ggtgcttcac cggtccggtg ctggtgacgg atcctgcacg
      301 atgaaacagg atgtcggagg cacttggggt cgtgaccgtg gtggttggca ttgtcttcct
      361 ccttgtcatg atgaatgtga gtcgcccaaa acggcgtcgt tgaagatccg cactgaaagg
      421 tcgcgtgccg tcggagattg gcacgcgacc ttgttggccg agaatcgggg cgacagtgtg
      481 ccttcggatg ggcaccatcc ggaggaaccc gctcgctgac agtcggcgtt tgccgcctgt
      541 cgcggtacta tctgcgccgc ttctcgccga tattgcccga agaaccggtc gtaacgtcat
      601 gcgcattcgc ctcagcaaca gccatgccag gtccggcact tttgaattca atgaagaatc
      661 ctaagtaatc gcgctgagcg ggacgacgag gaagtttgtg acgaggcgca gtcgagatga
      721 aaggtatccg aaacaatacc ggcgttatct caggagatac atcaagagaa ggcccggtgg
      781 gaggtagaag ttgtgggaga gctattggtg caggtgcttc atcgccgccg cacagaggat
      841 gatgaagaaa cccatccaaa gggccgcacg ctgaaaaggt atgacctcgt aggattcgcc
      901 ctcttcggca tcatcatccg acttgaagat taccgtgtag aggaacagcg tcaggaggca
      961 gagcacgagg aagagcttga taaagaatat tgtcaggtat tttgcgtgat gttggacacc
     1021 tgaaccggtc tgtgaggtga ggacttgatt gacgtaatgg aggttgatcc cacccgtaaa
     1081 caagagaatc acaaggagga ttcccgccac tttcttgtaa tgctgataga agacatcggt
     1141 gatgggcttg atctgctccc ttgcgacggc cttctggagg ccaggggtga cggccatgac
     1201 gaggaaagcc agtccgccga tccaaatgac cgcggacaac agatgaaacc agtggttcac
     1261 gatcggaatg agcgtattga gatcggtgac gatgctttgg aaggcgtcca tagaaatctt
     1321 tattcgtgaa gcctgaagcg tgaagcgaga gtgcgattct tcatgcagtc attcgcgcga
     1381 gtacgcttca ccaaaggcga gaagtgctta gaacttgaag accggtgcgt cgttgccgaa
     1441 gcgcttcttt cgaagctctt ccatcagttc gatggtcacg gtttcaatgc cgtgttcccg
     1501 tgcatgcttc tcaacgccct tctttaccat ggcgcggaga aaatacggga tgcggctcaa
     1561 tcgcaacgag gccgcattgt cccactgcac ggttgacgac tcttcgacgt tgaacgcgac
     1621 cttgatcttc tcgccacctt tcggcgtgta aaggcaccac tggtcctcat ccaaccaatc
     1681 cccatgatcg acataaggac gggcccggca acctccgcag atgtcggaat actcacagtc
     1741 gccgcacttg cccttcagct gaggataccg gaacgaatta aagatgtcgg atttctccca
     1801 cagctcgacg aagctgttct ctcggacatt gcccgcagag agcggcatgt agggacaggg
     1861 ggtcagttca ccgttaggtg tcacacgagc gtaattagtc ccggccaggc agccaccgcc
     1921 catgtatccc gtcgccttcg tgatcggtga attcggatcc ttctcatagg ccagccgttt
     1981 gaagtgcgga gcgcagcggg cccgaatcaa catcccctga tactcatcct gacacttcac
     2041 caggtagccc aaaacctcct cgtattgggc gggggtaatg tccgtcaatt cttcacctcg
     2101 gcccgtgcag accatgaaga agacgttgag tacccgtgct ccgaggccat gagcccattc
     2161 cactacggcc ggcagttcct gataattcat tggttgcgcg ctgaagtgca cctggaactg
     2221 cagcccgttg cgcttgctgg cttcaatgcc cgcgaccgcc gcttcccacg ctccaggcac
     2281 gccacgaaaa gcgttgtgct tcatcgaatc aagtgaatcg atgctgatgc cgacacccat
     2341 caccccgatg tcgaccagtt cttttgccat ccggtcgtcg atcagcatgc cgttcgtacc
     2401 gaacaccacc ataaagccaa gtttcacggc atgacgcgcg atctcgagaa tgtcgggacg
     2461 caccagcggc tcaccgccgg ttatcaccag cagacatcct ttgttgacct cggcgatctg
     2521 atcaatcaaa cggaaacact cttcagtcga aagttcatcg tcaccgcctg ccgccttggt
     2581 ggtcgcatcg aggtaacaat ggtcgcactt gagattacac cgcttcgtta gattgagcgc
     2641 gaccaagtag ggtttgaagt catcgacggt ccggccgtca aatggaccct cggtctgcgg
     2701 ctttggtgtc aagaattgcg tgagctgttg ctgaagggac ccaatcgctc ctgagaggga
     2761 tggaccgttg aagataggga gggatttacc catgagcgtg gttaatatgg accagagaaa
     2821 tcgttatacg agtaagttca aagtggacaa gcaccgggcc attcgacgtc actcatggcc
     2881 attgcgccta cgctctgcct ttgcctgtga gattggcgat catgtccttg aggttgccgg
     2941 tcttcatggc ttcttccatg tagccctttg cttgctcgat cccttcgttg gcgacttcca
     3001 acgagatgtg ggtcgtaccg atcttctcgg catgcttgat gatgagtgcc tttgtgcagt
     3061 ctcgcatgaa gccttccggc gcccgctcga gtcgtgcctg tgcctccgct gtccagttgt
     3121 aggcagacgc ttctgcgccc ccgttgccgt tttccggtga ggcgacatcg cgcattccgt
     3181 tcccattggc ctgggcaagc ttcgcctcgg cagcggccga cgtgcccgcg cccttgttgg
     3241 cgaaaatcgg ctgatagtct tcggaagcgg cttcctggat catcggtgcc gcgtactcca
     3301 aggtgatggt cgtcatgccg agcttccgcg cgctcttttc gatcttcgcc ttggcgcggc
     3361 gccgctggaa tcctgccggt acggcacgga tggcttcttt agcat
//
LOCUS       DASTQY010000083        10165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1757308, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000083 DASTQY010000000
VERSION     DASTQY010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10165
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757308"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(414..587)
                     /locus_tag="VFR82_02670"
     CDS             complement(414..587)
                     /locus_tag="VFR82_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500529.1"
                     /translation="MSSTITLVVPRVETESVFLQLVVQAAIRAHRDDYPKSHEVKTDT
                     VNDDRSRVFPVAV"
     gene            965..1552
                     /locus_tag="VFR82_02675"
     CDS             965..1552
                     /locus_tag="VFR82_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500530.1"
                     /translation="MVIPQPMTLVQQPEPFDDWDWVFELKHDGFRALAGIEHGQCRFF
                     SRKKHKLVGYQDLRTALVKEVHAETAILDGELGVTDHMGRTVFADLMQRRNLARYFAF
                     DLLCHNGGDLRKVALLTRKEKLKWISPARSAHALYVDHSRGCGSALYRLACQLDLEGI
                     VAKRADSRYEDNPNLRNWIKIKNLGYSQRERRGDL"
     gene            complement(1787..1861)
                     /locus_tag="VFR82_02680"
     tRNA            complement(1787..1861)
                     /locus_tag="VFR82_02680"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1827..1829),aa:Thr,seq:tgt)
     gene            complement(1948..2175)
                     /locus_tag="VFR82_02685"
     CDS             complement(1948..2175)
                     /locus_tag="VFR82_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500531.1"
                     /translation="MTATDCLMLDAKHAILDEHHRRFQALHEEGRLDEAMKQFHITVN
                     CANDVLNDALNLVDEILEAHRKMNLPSPPLP"
     gene            2395..2622
                     /locus_tag="VFR82_02690"
     CDS             2395..2622
                     /locus_tag="VFR82_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500532.1"
                     /translation="MRSLCMLAVAVLTLAACSSTEWVHPNKPSSEYINDYNKCQNLVL
                     RDPKLQQGSQLMILNATERCVQKEGWRLVEQ"
     gene            2803..3186
                     /locus_tag="VFR82_02695"
     CDS             2803..3186
                     /locus_tag="VFR82_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4500533.1"
                     /translation="MLTVDQLMTKSLVNIESGRSAIEAAKLMKTHKVGSVFVQKDNRI
                     IGIVTEPDIIRKVVGAERVPYYVPVEEIMSSPVIGIDWRRPITEAADMMERYKTRHLA
                     VLNGGSVVGILSVRDLLHPVSVDEL"
     gene            3378..3620
                     /locus_tag="VFR82_02700"
     CDS             3378..3620
                     /locus_tag="VFR82_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500534.1"
                     /translation="MTTVLLDQKPIAPRVHTLEQTILDRLEEESVVGLDELIEMLPQY
                     SWNQIFHAVDQLARFGKIVLRRHRYDYTLFSTSYAA"
     gene            3975..5429
                     /locus_tag="VFR82_02705"
     CDS             3975..5429
                     /locus_tag="VFR82_02705"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEU4500535.1"
                     /translation="MRINPCALLLWVVMMTPVALTYGQEPPMPGVASPKPPGPIKPAQ
                     ETKTPSADATSLEELMLEKGLISQDDYIRLKAEQERRLSESATMAEFTASPRWYERLR
                     HYGYGQLRYNQLGNPNGQVRTYHDASVGDASNGAQPGFFYRRIRWVITGQVSEHVGIF
                     FQPDLASNVSGNTHVVSMRDAWGEWYFDKDKEYRFRAGLQRVPASWDNWQASRQRMAI
                     DRADATNSGAQSERDLGLSFQWSPKFAQQRWKHMIDYLYGAGDWGMVNISVYNGQGLN
                     AQETNKDKHVGIRLAYPFELPGGRLFEVGMNAYRGQFSVNRGTPAAGTTLWSNFDAGH
                     ASGKYLDERLNFFIYYPPQPWGFIAEYVVGRGPERDPNTGVIGEKSLYGGYVQGHYTW
                     QYSDVGLANFYVRYQAYNGGLKFQAGAPSDNMQETEIGVAWMPDPQWEITTAYTFTER
                     RNVFTTTPGTATTPGRQFDAYGNLIRLQLIWFFN"
     gene            5436..6419
                     /locus_tag="VFR82_02710"
     CDS             5436..6419
                     /locus_tag="VFR82_02710"
                     /inference="COORDINATES: protein motif:HMM:NF017765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HEU4500536.1"
                     /translation="MTVLEAMQSNLLTPMPLFFALGLLAKLVGSDLKVPDAIYTALVL
                     YLLTAIGFRGGAEIHEVGIGTIWAALLAAAFLGILIPCVGFVVLRQLGRFSIHDAAAV
                     AGHYGSVSAVTFAAASQFLTSLNIHPEPYMSAFLAIMEPIGIVAAIFLARAALQLESV
                     PGTRWLRPVLHEVLTGKGSLILLGALVIGYLSGPDGQAMTKPFFIDPFKGVLCLFMLE
                     MGLLAAQRLAEVRRVGFFLVAFAVCMPILYGLFGVVAGGAVGLSIAGATLLGVLAASA
                     SYIAAPAAMRISLPEANPSLYLTASLGITFPFNLAFGIPLYFWFSTRLWGV"
     gene            complement(6405..6977)
                     /locus_tag="VFR82_02715"
     CDS             complement(6405..6977)
                     /locus_tag="VFR82_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500537.1"
                     /translation="MDKAAETAHSHPYVFKPADMETILSGVQVEERDTITGTGLLGSK
                     YAKPAFTQAEIARVSGHLVEALKKASPRDLTTFYMVVNDGQHKRSITSGGVFVDGKRR
                     LHVILANWRSAPSGGQDYTMAMELDTRDEPLLPISPFRFRVGFLHENAWLKSRSDRGD
                     RSFPSYRSVYVDPAKSVVIDLDVLLHSNTP"
     gene            complement(7198..8283)
                     /locus_tag="VFR82_02720"
     CDS             complement(7198..8283)
                     /locus_tag="VFR82_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU4500538.1"
                     /translation="MNSPLTVVALVLFMVALTLPLYAEQGHMARTTASIQPDLESYVA
                     QTSVQGRMTIAGSDTMQPVLSKLAMDFRRRQPDMKIAVQGTRDSKLSPEDVFLTGLST
                     MRRGDGDTAGHFGAYDVQLLASSRPLTEEELKRFQSRYGYPPTAVAIAQDAVAVYVNQ
                     DNPISQLTLSQVDAIFSSTRKRGLADRQEWGQAGLDGAWEHAPIRLYGRDQRSTGTLP
                     FFKQVVMQGGEFKKGLIPEPGSASVALAIGKDREGIGYSGIGFQTSAIKAVPLSEGPG
                     SPAIAPSIESVMDGQYPLSRPLYLYVNKDPKEQWEPKILEFLRFINSREGQETVARTG
                     IYPLSAPQVTHNLALLQKNHIQAAVRK"
     gene            complement(8437..9360)
                     /gene="gspK"
                     /locus_tag="VFR82_02725"
     CDS             complement(8437..9360)
                     /gene="gspK"
                     /locus_tag="VFR82_02725"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HEU4500539.1"
                     /translation="MALLLALLVLALLVALILEFDTDARREYRDAAAFRDNFKATVLT
                     RAAVQAARAVLQQDHLRDRQTGQAYDSTTDLWAFPIEKYAIGDGLMTAQIEDERSKLN
                     LNDLAAGVDPNVKKTKMLRLKRLFNLLQINPDLVDAIADWVDADDIPEAAGAETVYYQ
                     SLRPSYRAANAPLQTLREIRLIKGMTPEVVDKLLRYVTVYPQEGESRVNINTADFLVL
                     QALDPRISQTMAGEIIQGRPFKTVQDLDRIGSFAEIAKELRLLSLYDVKSNMFSAQMT
                     LTVNETTKSGAVVLQRDEATGNSMVRYFRFM"
     gene            complement(9449..9997)
                     /gene="gspM"
                     /locus_tag="VFR82_02730"
     CDS             complement(9449..9997)
                     /gene="gspM"
                     /locus_tag="VFR82_02730"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEU4500540.1"
                     /translation="MIATLQERWRHLASRERIIVASGGAVVLASLLFLLVVDPLFTRI
                     DKLERQAVRKSKERVELSALASEYAAKQARITKLEQRLPASEGQFSLLAFMEDAAASV
                     QIRDRIVGMQPQQATTQQGYEETAVDLRLDGVQLPQLLGLLVALEQAPYDVQVHHLQI
                     KPKYDNPINLDATLRIVSYAKS"
BASE COUNT         2319 a         2690 c         2845 g         2311 t
ORIGIN      
        1 aaagcagagg acagagtttg acgggattag aggccgccgt caagcggaga ggcgagtgat
       61 aaaagcggtg gggacattcg aggaggaact acttgagaga gaagaaggca caccatcggg
      121 caacgacagt tgcatctcca tgagcaattg gtcgcgatgt gctttacaaa tgccgtgaga
      181 atggagttcc cgcgacgacg aagagtcatc tagtgtcttg taaagaacgg ctggtcttcc
      241 ttcttgttca caccaagcac ataaaatgat caaggaagca ccctccatcg accacagcgt
      301 aagaggatgc gggaaatcca tgacctgctc actaccctag gtgaagagca aaatatcact
      361 gcccaaaaaa cggcactacg aatgaggccg atgcagcgaa gcttggtatg tacttataca
      421 gcgaccggaa acactctact gcggtcgtcg ttgacagtat ctgtcttcac ttcgtgactc
      481 ttcgggtaat catcacgatg cgcgcgaatg gctgcttgca caacaagttg gagaaaaaca
      541 gattccgtct caacacgtgg aaccacgagg gtaatggtgg atgacatgag agaactcctt
      601 tccgcattcc ttgctttctt cgcctcggac gaaattgaaa agctgaggca aggagcgatg
      661 gctgtaactg tatcaatcga atcaggaatc gcgttgaagc ctgaggggtt gtcgctcgac
      721 agtcagaggg ggaaacagcc ttggccttac ttggggggtc gcagccatga cgatagcgag
      781 agacttcgca ttctctcggg cgcggtgtgg tcattggcaa gacctcacgt tatggatcgc
      841 gctttcgcca ggaccgcagg taaagggctg ttagcctatg cccccaagaa gcaggtgtca
      901 attagctgag aagtggcaat gggagccatt tttcggcgtg tcaacgcggt tgatatattg
      961 tctcatggtc attccgcagc ctatgacgtt ggttcaacag ccagagccat tcgatgactg
     1021 ggactgggtt ttcgaactta agcacgatgg atttcgcgcc ttggcgggca tcgagcatgg
     1081 acagtgccgg ttcttttcta ggaagaaaca caagctagtg ggctatcaag accttcgaac
     1141 cgcattggtg aaagaggtcc atgcggaaac ggcgatcctg gatggtgagc taggggtcac
     1201 ggatcacatg ggccggacgg tctttgccga tctcatgcaa cggcggaacc tcgctcggta
     1261 cttcgccttt gatttgcttt gtcacaacgg gggagattta aggaaggtgg ctttattgac
     1321 gcgaaaggaa aaactcaaat ggatctcgcc ggcgcgatct gcccatgccc tctatgtcga
     1381 ccattcgcgc ggctgtggta gcgcactata tcgcctggct tgtcagttgg acctggaagg
     1441 catcgtggca aaacgggcag acagtaggta tgaggacaac ccaaatctac ggaattggat
     1501 caaaatcaaa aatcttggct acagtcagag ggaaaggagg ggagacctgt aattcaagcg
     1561 cgcaggttag ctttgaaact atttaacctt ggatgctttc cacccgatct tagaaccctt
     1621 acgcagattg catggaggac aagctagtgc gatgttgtaa gaccagtgag ttccgcctcg
     1681 gctaatagga aagatgtgct caacatgata gtggcgcaag ggctcataac aataaaaaca
     1741 acgccccgac tcgggccctg aatgtgatcg taagtgctta aagttctgga gctggcgaga
     1801 ggaattgaac ctccaacctg tggtttacaa aaccactgct ctgccattga gctacgccag
     1861 ccagctagat tgctgaggga agaacgattg attattgagc cgagaacgta gcaactgctt
     1921 agaagcctgt caagcaatgc gccgccgtta ggggagcggg ggagagggca aattcatctt
     1981 ccggtgggcc tcaagaatct cgtccacaag atttagcgcg tcattcaaga catcattcgc
     2041 gcaattcacg gtgatgtgaa actgcttcat ggcctcgtcc aggcgtccct cctcgtgcaa
     2101 cgcctgaaat ctgcggtgat gctcatcaag aatggcgtgc ttggcgtcca gcatcaggca
     2161 atcggtagct gtcatgtatg ctccttgcag cgatgagcgc tcactctagc aaagccggct
     2221 gcggcgaaca atcatacgtt tgggtgggta ggcaaggggt gagatttacg tccgacacga
     2281 tgaaaagacc gactggtctt gagcaataga gcacacaaga agggccgatg cggcttgacg
     2341 gcgattgacg gacggtttaa ggtgccgcca ctgcaacccg aacgggaggg cttcatgcgt
     2401 tctttgtgta tgcttgccgt tgcggtactg acgttggccg cctgttcttc taccgagtgg
     2461 gtgcatccca acaagccatc ctccgaatac atcaacgact acaacaagtg ccaaaactta
     2521 gtccttcgcg atcccaagct ccagcaaggc agccagctta tgatcttgaa cgccaccgag
     2581 cgttgtgtgc agaaggaagg ctggcggctc gtggagcagt gaccaagccc gactcaacgg
     2641 ttttcttgca gtccgacccc tctcgttcat ctccccgaaa aagttaacac tctgtttacg
     2701 aatcgttgat ccggccggaa caacaggtcg gtagtctttc cacatgcaaa acagcagctc
     2761 acatgtcaaa ccgtctccac gactcaccct aaagaggaaa tcatgctcac tgttgatcag
     2821 ttaatgacaa agagtctagt gaacattgag tccggcagat cggccatcga agctgcaaag
     2881 ctaatgaaaa ctcacaaagt cggcagcgtg ttcgtacaaa aggacaatcg cataatcggc
     2941 atagtcactg agccggacat catccgcaag gtcgtcggcg ccgaacgggt cccttattat
     3001 gtgccggtag aagaaattat gagcagtcca gtcatcggta tcgattggcg gcgtccgatc
     3061 acagaggcag cggacatgat ggaacgatat aagacccgac acttggcagt gctgaatggt
     3121 gggtcagttg tcggaattct atcagtcagg gatctcttgc atcctgtatc cgtggacgaa
     3181 ttgtaagccc agagacgacc atgcccatgg aaaggccaag catgtttgat gatgcgctga
     3241 cgcacataat cgcatcgagt gtcgaagtag cgacgggtgc tgcggtggtg tacttcatct
     3301 attcgatttg ccgagatgtt agaaatgcat ttctccgata agaccctctg aatgctcaac
     3361 aactcttgag gtgtcccatg accacagttt tactagatca aaagcccatt gcaccgcgtg
     3421 tccatacact ggaacagaca atccttgaca gactggaaga agagtctgtt gtcggcttgg
     3481 acgagctgat tgagatgctc ccccaataca gctggaatca aatctttcac gcagtagacc
     3541 agttggcgcg ttttggaaaa attgtgctac gtcgccatcg ctatgactac acattgttct
     3601 ccacaagcta cgccgcctga ggaagacaac ggcctcagcg acgttcatct tgctgtgcat
     3661 tccacgtcac ctcagacaat ccacacaccc catcgttctc tcatctattc acctttcgag
     3721 tcggcccgcc taaaacaccg agtgacggga ttctgtgcgt cttggcgcga tgttgactgt
     3781 cggcctactt caatggactt gtttggtgcg gtggactagc gcattcccta aggtctttgg
     3841 gtcccctgaa aacatctatt aagcgatagg aaagttaaca agtgtgtaac aactcgttca
     3901 ttgccacgca atcttagtgc cttagcgtgg gcaggtgata aggagaatcc gttaccatca
     3961 agaggagggc attcatgagg ataaatccgt gtgcattgct cttgtgggtg gtaatgatga
     4021 cgccggtggc tctcacctac ggacaggagc cgccgatgcc cggcgtcgcc agtcctaagc
     4081 cacccggtcc catcaagccg gcccaagaga caaagacacc ttccgccgat gccacctctc
     4141 ttgaggagct catgttggag aaaggcctta tttcacagga cgactacatt cggctcaaag
     4201 cagagcagga acgccgactt tcagaatcgg cgacgatggc ggagttcacc gccagcccgc
     4261 gatggtatga acggctgcgg cattatggct acggccagct tcggtacaac caactgggca
     4321 atcccaatgg tcaagtccgc acttatcatg atgcatcagt cggcgatgcg agcaacgggg
     4381 cacagccggg gtttttctac cgtcgaatcc gctgggtcat caccggtcaa gtgtccgaac
     4441 acgtcggcat ctttttccaa cctgatctag ccagtaacgt cagcggcaac acccacgttg
     4501 tttcaatgcg cgacgcatgg ggagaatggt atttcgataa ggacaaagaa taccgcttcc
     4561 gagccggtct ccagcgcgtg cctgcatcct gggacaactg gcaagcttcc cgccagcgaa
     4621 tggcaatcga tcgtgcggat gccactaatt cgggcgcgca gagcgaacgc gacctcggtc
     4681 tctcgtttca gtggtcgcca aaatttgccc aacagcggtg gaagcacatg atcgactacc
     4741 tgtacggtgc cggagactgg ggcatggtca atatttccgt ctacaacggt caggggttga
     4801 atgcgcagga aacgaacaaa gacaaacacg tcggcattcg gctggcgtat cccttcgagt
     4861 tgccgggagg tcgtctattc gaggtaggca tgaacgcata ccggggccag tttagcgtca
     4921 acagaggaac gccggcggca ggaacgacct tgtggtcgaa ttttgacgct ggacatgcct
     4981 caggaaaata tctcgatgaa cgcttgaact ttttcatcta ttacccaccc caaccttggg
     5041 gtttcattgc ggaatatgtg gtgggtcgag ggcctgaacg cgatcccaat accggggtca
     5101 ttggggagaa aagcctgtac gggggctatg tccaagggca ctacacgtgg caatacagcg
     5161 atgtcggatt ggccaacttt tatgttcgat atcaagccta caatggcggc ttgaagttcc
     5221 aggccggcgc gccaagcgac aacatgcagg aaaccgaaat cggtgtcgcg tggatgccgg
     5281 atccccaatg ggagatcaca acggcgtaca ccttcactga acgacgtaac gtgtttacaa
     5341 ccacaccggg gaccgcgacg acgccgggcc gccagttcga tgcatatggc aacctgatac
     5401 gccttcaact gatatggttt ttcaactgag gtactatgac ggtcttagaa gccatgcaga
     5461 gcaatttgct gacgccgatg ccgctatttt ttgcactcgg tctgttggcc aagctggtcg
     5521 gctccgatct caaagttccg gacgcgattt ataccgccct cgtgttgtat ctcttgacgg
     5581 ctatcggctt cagaggcggt gctgagatcc atgaagtggg aattggaacg atttgggcgg
     5641 cattgttggc cgcggcattc cttgggatcc taattccctg tgtcggattt gtggttctga
     5701 gacaactggg ccgtttctca atccacgatg cagcggcggt cgcgggacac tacggatcgg
     5761 tcagtgcggt tacctttgcc gcggcgtcac aattcctcac gtcgctgaac atccatcccg
     5821 aaccctatat gtcggcgttc ctcgcgatca tggaacccat cgggatcgtc gccgccattt
     5881 ttttggcccg agcggcactg cagctagaat cggtgccggg cactcgctgg ctccggccgg
     5941 tgcttcatga agtcttgacc gggaagggaa gcctaattct cctcggcgca ctggttatcg
     6001 gctatctgag cggccctgat ggccaggcta tgaccaagcc attttttatc gatcccttta
     6061 agggtgtgct gtgcctgttc atgttggaga tgggtctgct ggctgcgcag cgtctcgcag
     6121 aggtgcgtcg ggttgggttt ttcttggtcg cctttgcagt ctgtatgccg attctgtatg
     6181 gcctcttcgg cgttgtcgcc ggaggagcgg ttgggctcag cattgcgggc gccacgctac
     6241 tgggcgtgtt ggcagcaagc gcctcctaca ttgcggctcc ggctgcgatg cgaatctctc
     6301 ttcccgaagc aaatccctct ttatatttga ccgcttcgct gggtatcaca tttcccttca
     6361 atcttgcctt tggcatcccc ttgtattttt ggttctcgac taggttatgg ggtgtttgaa
     6421 tgcagcaaca catcgaggtc gatcaccact gatttggccg ggtcgacata gactgaacgg
     6481 tacgagggaa aactccgatc gccccgatca ctacggctct tgagccacgc gttctcgtga
     6541 agaaatccca cccgaaaacg aaacggtgaa atgggtagca gcggctcatc gcgggtatcc
     6601 agttccatgg ccatcgtgta gtcctggcca ccgctgggcg ccgacctcca attcgctagt
     6661 atcacatgga gccgtcgctt tccgtccacg aagaccccgc ccgacgtgat tgagcgtttg
     6721 tgctgaccgt cgttcacgac catatagaat gtagtcaggt ctctgggtga agcttttttc
     6781 aacgcttcca ctaagtgccc actcactcgc gcaatctccg cttgagtgaa ggccggtttt
     6841 gcgtatttcg atcccagaag tccggtgccg gtgatcgtgt ctcgttcttc gacctgaacg
     6901 ccgctgagga ttgtttccat gtcagcaggt ttgaacacat agggatggct atgcgcagtt
     6961 tcagcggctt tatccacggt aatccacacg cccgtcagtg gagtatcgtg aatgggtatt
     7021 gaactgggga gaggggcggc gcagcctgac acccacaata tcgtcgctac gactatgtgg
     7081 tggagtgatg gtctattcat aacggtcggc acgtgacgga gaagtctagc ctgcgataga
     7141 caaaaaagaa aggcatctgg aggagtggct cccagatgcc tctttcactt gcatgcgcta
     7201 cttgcgaacg gcggcctgaa tgtggttctt ctgaagaagg gcaagattgt gagtcacttg
     7261 cggggcagaa agaggataga tgccggtgcg agccaccgtt tcctgtcctt cgcggctatt
     7321 gatgaagcgc aagaattcaa ggatcttggg ctcccactgt tccttagggt ctttgttaac
     7381 atacaagtac aaaggccggc tcagcgggta ctgaccgtcc atcacggact caattgaagg
     7441 cgcaattgcc ggcgagcccg gcccttcact gaggggcacg gctttgatcg cagacgtttg
     7501 gaatccgatc ccgctgtatc cgattccctc acgatccttt cctattgcca aggcgacgga
     7561 ggctgaacct ggctcaggga tcaggccctt tttgaattct cctccctgca tcacgacctg
     7621 tttgaagaag ggcagggtac cggtcgaacg ctggtcacga ccatacagcc ggataggcgc
     7681 atgttcccat gctccatcca accctgcctg accccattcc tgtctgtctg ccaagccgcg
     7741 cttccgagta cttgagaaaa tggcatccac ctggctcaac gtcagttggc tgataggatt
     7801 gtcctgattg acatagacag ccacggcatc ctgggcgata gccacggcgg ttggcggata
     7861 gccatatcgc gactggaatc gcttcagttc ttcctcagtc agcggccgtg acgaagccag
     7921 cagttgcacg tcgtacgccc caaaatgtcc ggcggtgtca ccatcgccgc ggcgcatcgt
     7981 ggagaggcca gtgagaaata catcctcggg gctcagcttg gaatcccgtg tcccttggac
     8041 ggcaatcttc atgtcaggtt gtctgcggcg gaaatccatc gccaacttgg acagcaccgg
     8101 ctgcatcgtg tcggaacccg cgatcgtcat gcgaccttgc accgaagtct gtgccacata
     8161 ggattcgagg tctggttgaa tggaggcagt ggtcctggcc atatgccctt gctctgcata
     8221 gagcgggagg gtcagcgcga ccatgaatag cacgagcgcc acgacggtca gcgggctatt
     8281 catggagcta ttcgtagaag tgatacgctt catatggtgg tgacctcctg tgatattgat
     8341 gtcaggataa tacctatcca ggatgtcgtc cggatgacgg cactgttaca gaattgttaa
     8401 ctttcgagga gaatgtgacg tagaagggtc gtttgctcac ataaagcgaa agtatcggac
     8461 catgctgttg ccggtcgctt cgtcccgctg aagcacgacc gcgccagatt tggtcgtctc
     8521 gttcaccgtt aaggtcatct gtgccgagaa catattgctt ttgacatcgt agaggctgag
     8581 caggcggagt tcttttgcga tttcagcgaa actgccgatg cggtccagat cttgaacagt
     8641 tttgaacggg cggccttgta tgatctcacc ggccatagtc tggctgatgc gtggatccag
     8701 cgcttgcaag accaaaaagt ccgccgtatt aatgttgacg cgactctcgc cttcctgggg
     8761 atagacggtc acatatcgaa gcaacttatc gacgacctct ggcgtcatgc ctttgatcaa
     8821 ccggatttca cgcagcgttt gcagcggagc attggccgcc cgataagacg gtcgaagcga
     8881 ctgataatag accgtctcag cgccggccgc ttcaggaatg tcatcggcat cgacccaatc
     8941 ggcaatggcg tcaacgagat cggggttgat ttgcagcaga ttgaacagcc gctttagcct
     9001 cagcattttc gtcttcttga cattgggatc gacgccggcc gccaaatcgt tcaggttcaa
     9061 tttgctccgc tcatcctcaa tctgagccgt cataaggccg tcgccgatcg catatttttc
     9121 gatgggaaaa gcccacaagt cggtcgtcga atcgtacgcc tgccccgttt gtctatcccg
     9181 cagatgatcc tgttgcaaca cggcgcgtgc ggcctgaacc gcagcccggg tgagcacggt
     9241 ggccttaaaa ttgtctcgga aggcagcggc gtcgcgatac tcgcgccggg catcggtgtc
     9301 gaattcgagg atcagggcga cgaggagagc caggacgagg agtgcgagga gcagagccac
     9361 accacgttcg tcatctctcg cgatggaacg aaggacaccg gcgtcgtgtc tgctctgcac
     9421 aggggatcgc cggcgaccag ccgaacggtc aagatttggc ataggacacg atgcggagcg
     9481 tcgcgtccag gttaatcggg ttgtcgtatt tgggcttgat ctggaggtgg tgaacctgga
     9541 catcgtacgg tgcctgctcc agtgcgacca acaggcccaa cagctgcggc aattggactc
     9601 catccaacct gaggtctact gccgtttcct cgtacccttg ctgggttgtc gcctgctgcg
     9661 gctgcatccc gacaatgcga tcccgaatct gtacagaagc cgcagcgtcc tccataaagg
     9721 ccagaagaga gaattgcccc tcgctggccg gaagccgttg ctccaacttg gtgatccggg
     9781 cttgttttgc ggcatactcg gatgccagcg ccgacaattc gacacgttcc ttcgattttc
     9841 tgaccgcctg acgctcaagc ttatcgatcc tcgtaaagag cggatcgacg acgagcaaaa
     9901 acaggagact cgccagcacg acggccccgc cgctcgccac gataatccgc tctcgcgagg
     9961 cgagatgccg ccagcgttcc tgaagggtag cgatcatgac cgttggaccg tgtaggtaag
    10021 gcgaaagacg acctggctcg gggcggcgcc aacccgcgta tcggtgaccg agaccgcctg
    10081 aaacagctgg ttagacgtga aggcttgttt gatcttttca acggaatcga atgagttggt
    10141 ttcgccttcg aggtggaccg aagct
//
LOCUS       DASTQY010000084         2004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_959784, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000084 DASTQY010000000
VERSION     DASTQY010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959784"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VFR82_02735"
     CDS             <1..885
                     /locus_tag="VFR82_02735"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEU4500541.1"
                     /translation="YHTADATLWYFHALDRYLSATDDRETLALLYPRLEEVVHHHRKG
                     TRFNIGVDKRDGLLAAGAPGYQLTWMDAKVDDWVVTPRRGKPVEIQALWYNALRLMIQ
                     WGSQLGAKTDLWLQMAEQAEASFHQRYWIESGGYLFDVVDGEKGDDRSLRPNQIFSLS
                     LSHPILRPERWRSVVDIVSERLLTPFGLRTLASDHPDYKPTYSGDLRARDAAYHQGTV
                     WPWLIGHYIDARLRVYPDKAESRQLLAKFNDHLHDAGIGTISEIFDAEPPFRPRGCIA
                     QAWSVAEVLRAYLKTKEE"
     gene            886..1647
                     /locus_tag="VFR82_02740"
     CDS             886..1647
                     /locus_tag="VFR82_02740"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEU4500542.1"
                     /translation="MTQGTPGRQQSQCSVHIGTSGWHYKHWVGPFYPAGLPAKDMLSW
                     YVHQFDTVELNNSFYHLPKPETFEAWRRATPASFCFAVKGSRYITHRKKLNDPETAIA
                     RFLEPVQVLGDKLGPILFQLPPRWSCNLPRLSTFLESLPRSHRYTFEFRDPSWHDSAV
                     YRTLGRYNAAFCLYELDGFEAPYRLTADFVYVRLHGPGRKYQGDYSTKQLQDWAQRIS
                     RWRKELEAVYIYFDNDQAGYAAKNAADLKRMVAGS"
     gene            1718..>2004
                     /locus_tag="VFR82_02745"
     CDS             1718..>2004
                     /locus_tag="VFR82_02745"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEU4500543.1"
                     /translation="METRASVADHPIHPMLIPFPIALWIFSLASDCIYLFGFGGPVWK
                     DVALYTMIGGIIGALAAAIPGYIDYRSLTDPITTRVAQMHMFINLGLVLLYT"
BASE COUNT          424 a          570 c          575 g          435 t
ORIGIN      
        1 taccacacag ccgacgccac actctggtat ttccatgccc tcgatcgata tctttcggcg
       61 accgatgatc gagaaaccct tgccttgctg tatccccggt tggaagaagt cgtgcatcac
      121 catcgcaagg gcacccggtt caacatcggt gtagataagc gtgacgggtt gttggctgcc
      181 ggagcgccag gataccagct cacctggatg gatgcgaagg tcgacgattg ggtggtcacg
      241 cctcgccgag gaaagccggt cgagattcaa gcgctctggt ataacgcgtt gcgattgatg
      301 atccagtggg gaagtcagct tggagcaaag accgatctgt ggcttcagat ggccgaacag
      361 gcggaggcct catttcatca acgctactgg atcgaatcgg gcgggtacct ctttgacgtg
      421 gtggacggtg aaaagggcga tgaccggagc cttcgcccga atcagatttt ctccctttcg
      481 ctgagccacc ccattctccg tcccgagcga tggcggtcgg tggtcgacat tgtcagtgaa
      541 cgcctgttga cgcccttcgg gttgcgcacc ctggcctcgg atcatccgga ctacaaaccg
      601 acctattccg gagatcttcg cgcccgcgac gccgcgtacc accagggaac cgtatggccg
      661 tggctcatcg gtcattatat tgatgctcgg ttgcgggtct acccggacaa ggcggaatcc
      721 cggcaactgt tggccaaatt caacgaccat cttcacgacg ccggcatcgg cacgatcagc
      781 gaaatatttg atgccgagcc gcctttccgg ccacggggct gcatcgccca ggcgtggagc
      841 gtagcggagg tgctcagagc gtatttaaaa accaaagagg aatgagtgac acagggaaca
      901 ccgggcaggc aacagtccca atgttccgtg cacatcggga cgtcgggatg gcattacaag
      961 cactgggtag gaccgtttta tccggccggc ctgcccgcca aggatatgtt gtcctggtat
     1021 gtccaccagt tcgacacggt ggagctgaac aacagcttct atcatttgcc gaagccggag
     1081 acgtttgaag cctggagacg ggcgactcct gcgtcattct gttttgcagt caaagggagc
     1141 cgctacatta cgcatcgcaa aaaattgaac gatcctgaga cagccattgc gcgttttctg
     1201 gagcccgtgc aagtactcgg tgacaagctc gggccaatcc tctttcagtt gccgccacgg
     1261 tggtcctgca acctcccgcg tctcagcacc tttctcgaat cgctgccccg gagccaccgt
     1321 tatacgttcg aattccgcga tcccagttgg catgattccg ccgtgtacag aaccttgggc
     1381 cggtacaatg cggctttctg cttgtatgaa ctcgacggct tcgaggcgcc gtaccgactg
     1441 acggcggact ttgtctatgt gcggcttcat ggtccgggac gtaaatacca aggcgattac
     1501 tcgaccaagc agttacagga ttgggcacag cggatctcac ggtggagaaa ggagctggaa
     1561 gcggtctata tatacttcga caacgaccag gccggatacg cagccaaaaa tgccgcagat
     1621 ctcaaacgga tggtggccgg ctcatgaagg gtatctacga tgggccctct gccggctcag
     1681 acctcaaatg ctcgagagga ttgtgaggag gtgacccatg gaaacacgtg ccagcgttgc
     1741 cgatcatcct atccatccca tgctgattcc ctttccgatc gcgctgtgga tcttttcgct
     1801 ggcgagcgac tgtatttatc tctttggctt cggcgggccg gtgtggaagg acgttgcctt
     1861 gtacacgatg atcggcggaa tcatcggcgc gctcgcggct gcgataccgg gatatatcga
     1921 ctaccggtcc ctgacagatc cgatcacgac ccgcgtagcg cagatgcaca tgttcatcaa
     1981 cctcggactg gttttactgt acac
//
LOCUS       DASTQY010000085         5699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1150711, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000085 DASTQY010000000
VERSION     DASTQY010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150711"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VFR82_02750"
     CDS             complement(<1..954)
                     /locus_tag="VFR82_02750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4500544.1"
                     /translation="MQPNSAVSTMEQPDTRRPSTGPLRVEVVRDYRTFLKLEPEWNRL
                     VEEAGIDFPFVRHEWVRADWDCFHHAATLYVIVVHDRGRPLAIAPLMLERVRTYGFPV
                     QRLRGIAHVYTERFDFILSVRPQECCEAIWTCLADHAHQWDVLELPHVPARSLTLDMF
                     PPVTARTRCALTRWPSAESPYVAVRETWDAYCKGLKKTHRHDMRKRVANLEQKGPLEL
                     EVILSDRCMDRDFEDALRLESSAWKGAEGTAIESRKDSISFYRDVIQIAVRNAWLRLY
                     FLKVGGVRIAVRIALLFQNKLYMLKAGYDPQYASYAPGHVLS"
     gene            complement(944..2050)
                     /locus_tag="VFR82_02755"
     CDS             complement(944..2050)
                     /locus_tag="VFR82_02755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4500545.1"
                     /translation="MPHITIVAPHVTKSGGGQALHAADLSDRLRREGVSVQLLPIDPD
                     FPHGARWVRRVPYLRTLLNQSLYLPSLLTLRRSDIVHVSSASYWSFLLAPLPAMIIGR
                     LLGKPVILNYHSGEAADHLGRWGVLVHPWLSLADEIVVPSEYLRQVFRSHGYCTRVIN
                     NTVDTSRFMYRVRDPLRPRLLSTRNFEWYYGVDHTLRAFKLIQRTCPDATLEIAGAGS
                     RERDLHALTRSLKLTGVRFLGPVAPSDVPALHERADILMNSSLIDNQPLSILEAMASG
                     MPVVSTNVGDVGNMIEHGVSGTIVPQKDPAAMAAAVVFLLKYQDAARAMASRAVERLG
                     RYSWNRVGCQWLELYSDVMQRRPSERPGGIRHAA"
     gene            complement(2061..3542)
                     /locus_tag="VFR82_02760"
     CDS             complement(2061..3542)
                     /locus_tag="VFR82_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEU4500546.1"
                     /translation="MLKPALTLMAGRTLGYAVLFLVPIILVRLFNQEEFGTYKQVFLL
                     YGTILNLAQVGMSESLFYFIPGSSDEAGRYVCNSILVLGGIGLLTGGLLYLGGDVIAQ
                     YLNNSALSPLMPLLAAYFFLMLASYVLEIVMTARHQYSYAAASYGLSDVARAALIVMP
                     ALLFQTVASVLYGAIVHALIRLSATIWYSVKQFGGGLRPDRSVLLRQIAYSLPFALYV
                     LFQTGQESLHLFVVSSWFDAATFAIYSVGCLQIPLAEVVSMSVVNVMMVGMVQAIRDG
                     REAAVVPMWHDTVRKLALMFFPFAAMLLITAHDLIIFLFTDAYAASVPIFRTWSLVVL
                     LSAVPMDGLLRVYAQTPFLLFINVVKFILVAGAIYWFVSGLGLIGPVVITLLALAAGK
                     AIGLARMIMCGHLNVGNVLPWRSLLEIGLVAGAAALPAWWITAQVSAPPVARVAMTAL
                     MYGSVYLGLALLCGVIRKNEQDRVLSWIQPAWRPMRFKALFMR"
     gene            complement(3535..4671)
                     /locus_tag="VFR82_02765"
     CDS             complement(3535..4671)
                     /locus_tag="VFR82_02765"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU4500547.1"
                     /translation="MLKALAKTGVAAVLGWTGADRAVSSLTGTLRLPLVVGYHAVVED
                     VRAHVGRAIAPNLISLNMLERQLEWIGRRYRFMTLDDLGAQFERREPFERPSAAVTFD
                     DGYVGVYRHALPLLKRMGIPAGVFIVTELTGRSELQVYDKIYLLLEKVLPLLGHSETR
                     LRQLLQTKNVSLGKRHGARRLEAFSTMRWLLNTQTQKGLRRVIEALETVSGIRDTDYP
                     DLQMMDWEMVRTMDASGITIGSHTQTHALLTEESLERAANQTVGSMYTLSDRLKRPIR
                     HFAYPDGRFNAAVVAAVAQAGYRYGYGTCLRRDLRYPNLTIPRTLLWERSCVDARGGF
                     SSSLMRCHAYRLTDLWAPCTHNHGNSAAASAASQRSEARQRLHA"
     gene            complement(4665..>5699)
                     /locus_tag="VFR82_02770"
     CDS             complement(4665..>5699)
                     /locus_tag="VFR82_02770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4500548.1"
                     /translation="FSPKRTLALITVRRQNRLLGLAPCFMETQRFGGIVPYPSLQFLG
                     TGLVGSDYLDVIIRRGHEQQVSRSLAEYFQSRRLVMTLSHVKQQGALAAGFVKELEQA
                     GWTVQRRPIDACPHIPLKGHTWESYLASLGSQHRYNFNRRLKNFQKEGTVIFDAVEKE
                     DQRREALETLIDLHNLRWDERGGSETWQSPAMRAFHEGFTRMALERGWLRLFILRLNG
                     CPVGALYGLLYGRRFYFYQSGFDPMYRQHSVGLLTMGLAIKQALAEGAEDYDLLHGKE
                     TYKFLWAKETAELDRLHLYHPGLGGAVCRRAVMLDELAKSAARRYLPPAVLEHAAAWR
                     RGEMLRSSSC"
BASE COUNT         1324 a         1690 c         1631 g         1054 t
ORIGIN      
        1 ggacaagaca tgtcccgggg cataggaggc atattgcgga tcgtatccgg cttttagcat
       61 gtacagtttg ttttgaaaca gcagcgcgat ccgcaccgcg atgcgaacac cgcccacctt
      121 caaaaaatac aaccgaagcc aagcgttgcg caccgctatc tgtatgacat cacgataaaa
      181 ggagatgctg tccttccggc tctcgatggc cgttccttcc gcgcccttcc aggccgaaga
      241 ttccaatcgc agcgcgtctt cgaaatcgcg gtccatgcag cgatccgaaa ggatgacttc
      301 cagctccagc ggacccttct gttccaggtt cgccacccgc tttctcatgt cgtggcggtg
      361 cgtttttttg aggcctttgc aataggcgtc ccatgtctcc cgcaccgcca catagggaga
      421 ctcggccgat ggccaacgag tgagggcaca ccgcgttcgc gctgtcaccg gaggaaacat
      481 gtcgagcgta aggcttctgg ccggtacgtg cggcagttcc agcacatccc actgatgcgc
      541 atgatcggcc agacacgtcc aaatagcttc gcagcactct tggggccgga ccgaaagaat
      601 gaaatcgaac cgctcggtgt agacgtgagc gatccctcgc aatcgctgta ccggaaatcc
      661 gtacgttctg acgcgttcca gcatgagcgg agcaatcgcc aagggacggc ctctgtcatg
      721 cacgacgatg acatagagtg ttgccgcatg atggaagcag tcccagtcgg ctcgaaccca
      781 ctcatgccgg acaaacggaa aatcaattcc cgcctcctcc accagtcgat tccactctgg
      841 ttcaagcttc aagaaggtgc ggtaatctct caccacttcg acacgcagcg gaccggtcga
      901 cggtcgccgg gtatctggct gttccatggt cgaaacggcg gagttaggct gcatgccgga
      961 ttccaccagg gcgctcgctt gggcgccgtt gcatcacatc cgagtaaagc tccaaccact
     1021 gacatccaac ccggttccag gaatagcgac cgagccgctc gacggcgcgg gaagccatcg
     1081 cgcgcgccgc atcctgatac ttgagcaaaa agaccacggc cgccgccatt gccgcgggat
     1141 ccttctgggg cacaatggtc ccgctgactc cgtgttcgat catgttgccg acgtcgccga
     1201 cattggtgct tacgacgggc atgcctgacg ccatggcttc caggatcgaa agcggctgat
     1261 tgtcgatgag agaggagttc atcaaaatgt cagctcgttc gtgcagagcc ggtacgtccg
     1321 agggagcgac tggccccagg aagcgcacgc cggtcagttt cagcgagcgg gtcagggcgt
     1381 gaaggtctct ttcgcgactt cccgcaccag cgatctccag cgtggcgtca ggacaggtgc
     1441 gctggataag cttgaaagca cgcaacgtgt gatccacccc gtagtaccat tcgaaattcc
     1501 gcgtggagag caaccggggt cgaaggggat cgcgtacccg atacatgaac cggcttgtat
     1561 cgacggtatt attaatgacc ctggtgcaat agccgtggga cctgaatacc tgacgcaagt
     1621 actctgaggg aacgacgatt tcgtccgcca gcgacaacca tggatgaacc agtactcccc
     1681 accggccgag atggtcggct gcctccccgc tgtgatagtt caggatcacc ggtttgccca
     1741 agagccgtcc gatgatcatg gccggaagtg gagcaagcag aaacgaccag taggaggctg
     1801 atgacacatg gacgatgtcg ctccggcgca gcgtcagcag gctggggagg tacagacttt
     1861 gattgaggag ggtgcgaaga tacgggaccc gccgcaccca acgggcgccg tgcggaaaat
     1921 ccggatcgat cggcagcaat tgtaccgaaa ccccttctcg tcgcaaacgg tcggacaagt
     1981 ccgccgcatg cagcgcttga ccgccgccgg acttcgttac atggggagcg acaatggtaa
     2041 tgtgtggcac gtatgccctc tcatctcata aagagcgctt tgaagcgcat ggggcgccac
     2101 gccggctgaa tccagctcaa cacacgatcc tgttcgtttt ttcgaatgac cccgcagagc
     2161 agtgcaagtc cgaggtaaac cgatccgtac atgagtgcgg tcatcgcgac tcgagctaca
     2221 ggaggagcgg aaacctgtgc ggtgatccac caggcaggaa gtgccgcggc tcccgccact
     2281 aagccgattt caagaagact gcgccatggc aggacattcc ccacattcag atgcccgcac
     2341 attatcatgc gtgccagccc gatggccttc cccgctgcca acgctagcag ggtgatcacg
     2401 accggtccga tcaagcccaa accagacaca aaccagtaga ttgcccccgc cacgaggatg
     2461 aatttgacga cattaatgaa caataagaat ggtgtttgcg catagactct gagcagtccg
     2521 tccataggaa cggccgacaa gaggaccacg agagaccagg ttctgaaaat cggaacgctg
     2581 gccgcgtagg cgtcggtgaa aaggaaaatg atgagatcgt gagcggtgat gagcagcatc
     2641 gccgcgaagg ggaaaaacat cagcgccagc tttcgaacag tgtcgtgcca catcggaacg
     2701 acggcggcct cacgaccatc ccggatagcc tgcaccatgc cgaccatcat gacattgacc
     2761 accgacatgc tgaccacctc cgccagaggg atctggaggc atccgacgga gtagatggcg
     2821 aaggtggcgg cgtcaaacca ggaggacacc acgaatagat gcaacgactc ctggccggtt
     2881 tgaaataaga cgtacaacgc gaagggcagg ctgtaggcaa tctgccgcag aaggaccgat
     2941 cggtcgggac gaaggccgcc gccgaactgc ttgacgctat accagatggt cgcgctgaga
     3001 cggatcagtg cgtgcacgat ggcgccatag agcacgctgg ccaccgtctg gaacagcagc
     3061 gccggcatga cgatcaaggc cgcgcgagcg acatccgata acccataaga cgccgccgca
     3121 taagaatatt ggtggcgagc cgtcataacg atctccaaca cataggatgc cagcatcagg
     3181 aagaaatatg ccgccagtaa tggcatcagc ggcgatagag ctgaattgtt gaggtactgc
     3241 gcgatcacgt ctcccccgag atacaagagg cccccggtaa ggagaccgat gccaccgaga
     3301 accaggatcg aattacagac ataacggcca gcttcgtctg atgagcccgg aatgaaataa
     3361 aacagactct ccgacatccc cacctgcgcc agattgagga tcgttccata gagcaagaac
     3421 acttgtttgt acgtgccgaa ctcttcctgg ttgaagagac ggacaaggat gatcggaaca
     3481 agaaacaaaa cggcataccc cagcgtccgg cctgccatga gggtcaacgc cggcttaagc
     3541 atgcagcctc tgccgggcct cggaccgttg ggaagctgcc gaggccgcgg cggaattgcc
     3601 atgattgtgc gtgcaaggag cccagagatc ggtcaggcgg taggcatgac agcgcatgag
     3661 cgaagaagaa aatccccctc gagcatcgac gcaggatcgt tcccacagta acgtacgggg
     3721 tatggtcaga ttggggtacc ggagatcacg ccgcagacaa gtgccgtagc cgtaccggta
     3781 tccggcttgg gcgacggcag ccacgacggc ggcattgaag cgtccgtcgg gataggcaaa
     3841 atgcctgatc gggcgcttca gccgatcgct cagtgtatac atggatccca ccgtctggtt
     3901 cgccgcacgc tcgaggctct cttcagtcaa cagcgcatgg gtttgcgtat gggatccgat
     3961 ggtgatacct gacgcgtcca tcgttcgcac catctcccaa tccatcatct gcaggtcagg
     4021 atagtccgtg tcccggatac ccgacaccgt ttctaacgcc tcgatcacgc ggcgaaggcc
     4081 tttctgcgtt tgggtattca acagccagcg catcgtgctg aaggcctcta gtcgccgcgc
     4141 tccgtggcgc ttgccgaggg agacgttctt ggtttgcaag agctgccgca atcgcgtctc
     4201 ggaatgaccg agaagcggaa ggactttctc caacagcaga tagatcttat cgtacacctg
     4261 gagttccgaa cggccagtca actccgtcac gatgaagaca ccggcaggga tgcccattcg
     4321 cttcaacagc ggcagggcat gacgatagac accaacataa ccgtcgtcga acgtcacggc
     4381 agcagagggc cgctcgaacg gttctcttcg ttcgaattgc gcaccgagat cgtccagcgt
     4441 catgaaacga tatcgtcgac cgatccactc caactggcgt tcgagcatgt tgaggctgat
     4501 cagattgggg gcaatcgcgc gtccgacgtg cgcgcggaca tcctcgacga ccgcatgata
     4561 tcccaccacc aggggaagcc tcagcgttcc cgttaacgag ctgaccgcgc gatcagcccc
     4621 cgtccatccc agcacggcgg cgacaccagt cttggctagg gcttttaaca tgaggaggac
     4681 cgcaacattt ctccgcgtct ccaggccgcg gcgtgttcga gaaccgccgg cggcagatac
     4741 cggcgggcgg cggatttggc cagctcatcg agcataacgg ctcgccgaca gaccgctcct
     4801 cctaaacccg gatgatagag atgcagccga tcaagttcgg ctgtttcttt cgcccataga
     4861 aacttatacg tttcctttcc atggaggagg tcgtagtcct ccgcaccttc cgcgagcgcc
     4921 tgcttgatgg caagacccat cgtgagtaga cccacgctgt gctgccgata catggggtcg
     4981 aatcctgact gatagaaata gaaccgtcga ccgtacagca acccgtacaa ggcccctacg
     5041 ggacaaccat tcagccggag gatgaagagc cgcagccatc cgcgctccaa tgccatgcgg
     5101 gtgaagccct catgaaaggc acgcatcgct ggcgactgcc aggtttcgga ccctccgcgc
     5161 tcatcccatc gaagattgtg cagatcgatc aaagtctcaa gcgcctcacg acgctggtct
     5221 tccttttcca ccgcatcgaa gatgaccgtg ccctctttct gaaaattttt gagtctgcga
     5281 ttgaaattgt accggtgctg cgatcccaag ctggcgagat aggactccca ggtatgtccc
     5341 ttcaagggaa tatgcgggca ggcatcgatc ggacggcgct gcacagtcca tccggcttgt
     5401 tccaattctt tgacgaatcc agcggcaaga gccccctgct gtttcacatg actgagggtc
     5461 atcaccaacc gacggctttg aaaatattcg gccaggctgc gggacacttg ttgttcatgt
     5521 ccccgtcgaa tgatcacgtc cagataatcg gaccctacga gtcccgttcc caggaactgg
     5581 agcgacggat aaggcacgat gccgccgaag cgttgggtct ccatgaagca gggagcaagg
     5641 ccgagcaagc ggttctgacg ccgcaccgtg atcaaggcca acgtccgttt gggggagaa
//
LOCUS       DASTQY010000086         2274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_745183, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000086 DASTQY010000000
VERSION     DASTQY010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745183"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1275)
                     /locus_tag="VFR82_02775"
     CDS             complement(139..1275)
                     /locus_tag="VFR82_02775"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEU4500549.1"
                     /translation="MQRRWVQLALGFSLFVLVVVVVSAFVDEPLRAYVEQRMNRSLKG
                     YTVRIGALNFHPIGFSVDLEDVELVRTDEPDPPMATIARWTARLHWKALLSGRVVNDQ
                     SIERPKFHITRKLARKEAGDDRPVRDRGWQDAVESVYPFKINQVRIANAELVYIDEAK
                     PDRPLHFDHVDVHATNIRNVQSGDQTYPSELHLEGQVFDEGKLRVDGHADFLADPYVA
                     VKADISLENVPLGTLVPVSTRYNLFLSGGSMSAEGSVEYAPTVKLVSLKRLRIDGLHA
                     DYVHARQTQRAEEARARATVSTAQQVHAKEDTLVRVEEITLVNSEFGFVNQATQPEYR
                     VFLADAEAGLQHYSNRLQEGAASMQLRGKFMGNGETSVTGILRP"
     gene            complement(1378..>2274)
                     /locus_tag="VFR82_02780"
     CDS             complement(1378..>2274)
                     /locus_tag="VFR82_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="HEU4500550.1"
                     /translation="ITLRLGRPRPLCRRRGNRIEYHWSLTENVVAATTPYGPLTVGPT
                     LQYGNDREPIALCTTAPRQVARIARAWKAIRLAWPEAHRVLELLTSRIVPLKARGVVS
                     FSYRHRPGLSFVNCFHRDNLDLIDDLIHENSHHHLNLLLRKHVLYRDDHNQQIFYSPW
                     RRSLRPVRGILHATFTFTMGAQLFERLSSWAETKPGLAQWREAGLSSGDLLRARFRCL
                     EEIESVRYSLRDLQYASGRLKWLTASGARLVSHLEQAIAKAERNISKHRHTVLASPYG
                     RLLDAHLTELREARHAYGPAHG"
BASE COUNT          497 a          682 c          615 g          480 t
ORIGIN      
        1 attcaacagg acttcggcat agcaggaaaa cgtcccgcct acagcgtcga actttccatg
       61 ggcacgcaag agattattca ttgcccgaat cggggtgccc gcaattttca cccttaattc
      121 gaagtccggg gactcatgct acggtcgcag aataccggtg acgcttgtct cgccgtttcc
      181 catgaatttc ccccgcagct gcatcgaagc ggccccttct tgcaatcgat tgctgtaatg
      241 ttggaggcca gcctccgcat cggccagaaa cacgcggtat tcaggctgtg tggcttgatt
      301 gacaaaccca aactcgctgt tgacgagggt gatttcctcc actcggacga gcgtatcttc
      361 cttcgcatga acctgttgag ccgtgctgac tgttgctctc gcccttgcct cctccgctcg
      421 ttgcgtctgt cttgcatgga cgtaatccgc atgcagaccg tcaatcctga gccgcttcaa
      481 gctcaccagc tttacagtcg gtgcatattc cacggaccct tcagctgaca tgctgcctcc
      541 actgaggaaa aggttgtatc gagtcgacac cggcacgagt gtacccaagg gtacgttctc
      601 taacgagatg tcggccttca cggcgacata agggtcggcc agaaaatcgg catgtccgtc
      661 cactcgaagc ttcccttcgt cgaatacctg cccttcgaga tgcagctcgg acgggtaagt
      721 ctggtcaccg gactgaacat tgcggatatt ggtcgcatgt acatccacat ggtcgaaatg
      781 aagcggccga tcgggcttcg cctcatctat atagaccagc tcggcattgg cgatcctcac
      841 ctgattgatt ttgaacggat agacgctttc caccgcatcc tgccaccccc ggtctctgac
      901 cggcctgtca tccccggctt cctttcttgc gagcttcctg gtgatgtgaa atttcggccg
      961 ctcaatagat tggtcattca cgacacgtcc ggaaagcagc gccttccaat ggagcctcgc
     1021 ggtccatcgt gcaatcgtgg ccatcggagg atcgggttca tccgttcgaa ccaattcaac
     1081 atcttcgaga tccaccgaga atccaatcgg atgaaaattc agggcgccga tgcggactgt
     1141 gtagcccttg agactgcggt tcatcctctg ctcgacatag gctcgcaggg gctcatcgac
     1201 gaaggcggag acgacgacga ccacaagcac aaacagacta aatcccagag cgagctgtac
     1261 ccaacgcctc tgcatgtcct ccactttagc aaggccatcg aaccagccgg agctaggaaa
     1321 agaagcagtc gaaggacacc actccgccga gcacacagca tgcgagcaaa tcctgactca
     1381 gccgtgagcc ggcccataag cgtggcgggc ttctcgaagc tctgtcaggt gcgcatccag
     1441 gagtcgaccg tacggcgagg caagcacggt gtgtcgatgc ttggaaatgt tgcgttcagc
     1501 cttcgcaatt gcctgttcaa ggtgactcac gagccgcgcc ccagaggcgg tcagccattt
     1561 gagacgaccg cttgcatact gaaggtcgcg aagggaatag cggacggatt cgatttcctc
     1621 caggcagcgg aatcgcgccc gcagcaggtc gccactggac aatcctgcct cgcgccattg
     1681 cgccaggccc ggcttcgttt cggcccaaga cgacaagcgt tcgaacaact gcgcacccat
     1741 ggtgaaggta aacgtcgcgt gcagaatccc gcgaaccggc cgcaggctcc tccgccacgg
     1801 cgaatagaaa atctgctggt tgtggtcgtc gcgatacagg acgtgctttc gtaacaacaa
     1861 atttaggtga tgatgactat tttcgtggat cagatcatca attaagtcaa ggttgtcccg
     1921 atgaaaacaa ttcacaaacg acagaccagg tcgatggcgg taactgaagc tgaccacacc
     1981 gcgagccttg agcggcacaa tacgggatgt caataattcc agaacgcgat gggcttcagg
     2041 ccaggccagg cggatggctt tccaagcccg tgcaatcctg gccacttgac gcggcgccgt
     2101 cgtgcacagt gcgatcggct cccgatcgtt cccgtactgc agcgtcggtc cgacggtgag
     2161 cggtccgtac ggcgtggtgg ccgctacaac gttctctgtc agcgaccagt gatactcaat
     2221 ccggtttccg cgacgcctgc agaggggcct aggccggccg agtctcagcg tgat
//
LOCUS       DASTQY010000087         8246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_144193, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000087 DASTQY010000000
VERSION     DASTQY010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8246
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144193"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..1084)
                     /locus_tag="VFR82_02785"
     CDS             complement(203..1084)
                     /locus_tag="VFR82_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU4500551.1"
                     /translation="MTHRLSPAFILLAVFALQTTGCATNPYTKRWQLLMVPQSYEANL
                     GAQAYQDVLSSPKVKISDNPSEVTPVQRVASRIIEAAKKSKYADSAKDFKWEVAVIKD
                     DSTKNAFALPGGKIAVYTGIFPVADNESGLAAIMGHEVVHALARHGSERMSQGVLAQI
                     GLVGASIAMGTQGFSPVTSQAAMTALGVGSQVGVLLPFSRAHESEADYIGLLLAADAG
                     YDPQEAVRVWQRMQQSSDGKQPQEFLSTHPGHETRIKRLTEKMPEALALYNQAQKAPV
                     AQLPPVGVATAAPSAQQ"
     gene            complement(1249..2997)
                     /locus_tag="VFR82_02790"
     CDS             complement(1249..2997)
                     /locus_tag="VFR82_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4500552.1"
                     /translation="MPGAYPRHRLFCLVLLLCLFLLSGWMIVPPAIIAEELQGSSAPH
                     IIERAKTDWESGAITPALETLDQGIEANPDALTLHKLRGDILATSRASTEALQAYETV
                     LGMQPSALDVRWAKWSLLVRSGQGEQSIEELRRIAAVDTRNPLIHLRLARELRKLDHL
                     EASLQSYKEAVELAPDMHGWRLAMARARFDVLDYEGAVTDVQYVLDHIPPGSPLELPA
                     NTLVTVFHGHSQDRGRRFDPVFTPKDITPDQLKQWALMRADAWRMFVAGRYHEAEPMY
                     RKLLQLNPRDPSAAHQFGLTLMQLGKCDEALKVFGKVSDLDPTEDQYNDIVFRMGQCL
                     VELERWEEAFMHFHTLYAQAVQFEIQNKDNALPPGIRVLDKKKIVRWLERVKPHIPAE
                     LANLDNGPAPQATSAQDAGEAPAPTKEELDAALDRLKPERALDTGGVLVGRDADFSWF
                     RFVIPASKVMRDDLPTGQHDFIPLNPGDTFLTTQQDIYLVFRLVSDSYDAIPLATQCF
                     LETTEATGHAGMIAQDHVLMAMSDQSGYFTLAPPEHGWTPGLYRCGLFAGERTSAYTQ
                     ADEVRFRVVQSNSSSS"
     gene            complement(3092..4420)
                     /locus_tag="VFR82_02795"
     CDS             complement(3092..4420)
                     /locus_tag="VFR82_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500553.1"
                     /translation="MHRVWRQLFLAARTNGRLRRLLLGTAYLAACMLFPTGHAWAEEE
                     KPTPLAGEAFDTEVLGEPVQVAPRDRKSVTAASFGIQYLPDGPSFYQVLPFGALYVWR
                     NSDDMRQRFRGTFSGPVNDLTMNVGSQSSTGWEFRFTLNTMIIPLGRSEYVEGQIIRD
                     VEVEWSYVFGGFGLAYRRLLDPGHQDNALELSLTYEPGFRWFKGTSKTSPNFIVPEDT
                     YEGRVHARVRRDALERNLMELPHRGYAFGGDFIYGHRSNWSTWGGGVFGLSNERTYLM
                     GSVFALAATGVPFVSSERHRLVASVYAGLGQNLDRFSTFRLPGRPTGYEWEALALPIL
                     PSVAFNELFPRRYGIAHLEYRYEPIFFVFPYIRGSWGLIEQPRFQPDGSIRYKMDAMP
                     ALSGGVVTGAPWRSQVEINYSYNFGIYSDHSGGPPLPGRHGFFVFWSKEL"
     gene            4595..5074
                     /gene="trmL"
                     /locus_tag="VFR82_02800"
     CDS             4595..5074
                     /gene="trmL"
                     /locus_tag="VFR82_02800"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEU4500554.1"
                     /translation="MFDVILYQPEIPPNTGNIIRLCANTGVRLHLVRPLGFSLDDRQM
                     RRAGLDYHEYATMKVHDDWAICAAHFKGRRIFAVSTKGSQRYDTNQYLVDDVFLFGPE
                     TRGLPADLLMQFPAERRIRLPMLPDSRSLNLSNAAAVVIYEAWRQIDYADKPTRCQP"
     gene            complement(5133..6371)
                     /locus_tag="VFR82_02805"
     CDS             complement(5133..6371)
                     /locus_tag="VFR82_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500555.1"
                     /translation="MTPDQARAQYNFLLTLCIRKAEAFGPLAFAFIKEHDLDALGLTP
                     EEQFNLFVATAETFAEEPKRYTHKLDCLQKALDILPTTSFFDRELARQLQHDIQRLKA
                     ELDLYSAAMKPERASPAAGKLPQRIVVETDMPDYYLSIAQQRAAAYYQEKYRLTKESK
                     IAQHFTSATRKFEPETMAVHKEFPGACAPFVAARSGAWHVMLPFDLKISRSPEDPLDA
                     ALRIWYAKTGYSFPLRYDMGRLCSYYDDQVLDIAMTDPHLLFVSAAPLRETNLGQVDR
                     PVANDVPFELALPRAFLEGTNSLGPYVQVGCNIKLWFDASSVSVLVQGAPDLHEYGLE
                     GAAGLLTRTYASEKTAAYAGAGSQKWQEGLSFNFVNMHLQLLPDVTAAVVPAHTPIFS
                     VYPVMARGQYQVTDARTLGH"
     gene            6534..>8246
                     /locus_tag="VFR82_02810"
     CDS             6534..>8246
                     /locus_tag="VFR82_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500556.1"
                     /translation="MNTLWPILLLVLSGCRLIGLAVHPISVGPIEFDPSTIESIGISL
                     PVLGGDEDCDAVARVSYRELGATSWKEALPLQRVRTDLLSRAVPSVFPIAEQFAGSVF
                     NLRPDSAYEVKLTIEDPDGGKTTRMGLVRTRGMPRDLPLSPHVVHVNSDDTLASALAR
                     AAPGEVITLAKGHYHGPFTVDRSGTAADPIIIKGHGREETVIESPGAEYGVLITGSHI
                     HVEDLAIGLSTWGIKIVNAEEVVIQRTHIRDVMYGINARSGVNRNAYICDNLLEGKGV
                     LWPDTSNRTWDFEGIVVTGTGHVICHNTLAGFGDALGLSQPTVIPNRAIDFYGNDVVW
                     GGDDGIELDYSERNVRAYRNRFGNVAMGISFQPVWGGPVYAFRNVIYNTAKAPYKLNQ
                     EPSGFFIVHNTSIRSGWAWLQYGELVSNFTFYNNLTIGTEKAVYLTPSIEIADIDYNG
                     WSPDGEFKFDYTWTGFSSLHRESPYERHGRLLTAPVFGEPVIQPPRFESFMPPPSALR
                     LHASSNAIDAGVRLPNINDDYCGEGPDLGSVEQGRAVPYYGVREPEHRRGNGIGGQRE
                     TAIAC"
BASE COUNT         1775 a         2284 c         2406 g         1781 t
ORIGIN      
        1 atgacttgtc caaaaagatc aatgacttaa ctgcaggccg gactgggagg gctagagaag
       61 ggctctggaa atcttccata ggaccctgga agttttccat atcggaggca aggcgaaccc
      121 ttccgtgtat gagttcagtg gactgctgta ccactcgcag cacgaaggcg cgaacgatca
      181 ttctgacgag cccgccgcct agctactgtt gcgctgatgg ggcggcagtt gccacgccga
      241 ctggaggaag ctgcgcgacc ggcgctttct gagcttgatt gtagagagcc agagcttcgg
      301 gcatcttttc ggtgagtcgt ttgattctgg tctcgtgtcc cgggtgggtc gagaggaatt
      361 cttgtggttg tttcccgtca gaggactgct gcattctttg ccacactcgc accgcttctt
      421 gggggtcgta gccggcgtct gccgccaaca gcaacccgat atagtccgct tcggactcat
      481 gggcacgact gaacggcaag agcactccca cctgtgaacc gacgcccaat gcggtcatgg
      541 cagcctgact cgtcaccgga ctgaatcctt gagttcccat ggcgatagag gctcccacca
      601 gaccgatctg agcgaggaca ccctggctca tgcgttccga tccatgacgg gcgagcgcgt
      661 gaacgacttc gtggcccatg atcgcggcca gcccggactc gttatccgcg acggggaaga
      721 tccccgtata caccgcgatt ttacctcccg gcagcgcaaa ggcgttcttg gtgctgtcgt
      781 ctttaatgac cgcgacctcc catttgaagt cttttgccga gtcggcatac ttggattttt
      841 tcgctgcctc gatgatacgt gaagccactc tttggactgg tgtgacttcg gagggattgt
      901 cggagatttt gaccttgggg ctgctcagga cgtcctgata cgcttgggcg cctagattcg
      961 cctcatagga ttgaggaacc atgagaagct gccagcgttt ggtataggga ttggtggcgc
     1021 agccggtcgt ctgcagagca aagacggcaa ggagaatgaa tgcaggtgac agccgatgcg
     1081 tcatgtcccc ctccttctta gaggctacaa gggacattct acgaggcgag gaggacacgc
     1141 ggtgattagg accttcccta gggaaggtaa catcgcagcc cggttcagct gctagatcct
     1201 tctgtgccac cgggataccc gcggtggaga gagcccgtga ccgagcgatt aggatgatga
     1261 tgagttcgac tgaacgacgc ggaagcgcac ttcgtccgct tgagtatagg cagatgtgcg
     1321 ttcaccggca aagagcccac agcggtagag gcccggcgtc cagccgtgct ccgggggagc
     1381 gagtgtaaag taccccgact ggtcgctcat ggccatgagc acatgatcct gagcgatcat
     1441 accggcatga cccgttgctt cggttgtttc aagaaagcat tgggtggcga gcggtatggc
     1501 atcataggaa tcagagacga gacgaaacac gagatagatg tcttgctggg tcgtgagaaa
     1561 cgtatcgccg gggttcagtg ggatgaaatc atgctggcca gtgggaagat cgtcgcgcat
     1621 tactttgctt gcaggaatca caaagcgaaa ccagctgaaa tccgcgtcgc ggccgaccag
     1681 cacgccaccg gtatccaggg cacgctcggg tttgagccgg tccaacgcgg catcgagttc
     1741 ttccttggtc ggggctggag cctccccagc gtcttgcgcc gacgtggctt ggggcgccgg
     1801 tccattgtcg aggttggcga gttcggcagg gatgtgtggt ttcacccgtt cgagccaccg
     1861 gacgattttc ttcttgtcga gcacacgtat cccgggaggg agcgcattgt ccttgttttg
     1921 tatctcgaac tgcacggctt gagcatacag ggtgtggaag tgcatgaacg cctcctccca
     1981 ccgttcgagt tccaccaagc attgacccat ccgaaagaca atgtcgttgt actggtcttc
     2041 ggtcggatcg aggtcagaca ccttgccgaa caccttgagc gcttcgtcac attttcccag
     2101 ctgcatgagg gtcaagccga attggtgagc ggcggacggg tcgcggggat tcaactgcaa
     2161 taacttccgg tacatcggct cggcttcgtg ataacggccg gccacaaaca ttctccaagc
     2221 gtccgcgcgc ataagcgccc attgcttcaa ctgatcgggt gtgatgtcct tgggggtaaa
     2281 caccggatcg aatcgacgcc ctcggtcttg tgaatgtccg tgaaacaccg tgaccaaggt
     2341 attggccggc aattctaggg gcgaaccggg cgggatatgg tcgagcacgt attggacgtc
     2401 ggtgacggcg ccttcatagt ccagaacgtc aaaccgtgcg cgggccattg cgagccgcca
     2461 cccgtgcatg tccggcgcga gttcgaccgc ttccttgtac gactggagtg acgcctcgag
     2521 gtgatcgagc tttcggagtt cccgggcgag acgcaggtgg atcaatggat tgcgggtgtc
     2581 gacggcggca atgcggcgta attcctcaat ggattgttcc ccttggcctg accgaacgag
     2641 cagactccac ttggcccatc gaacgtccag ggcactcggc tgcatgccga gaactgtctc
     2701 ataggcttgg agcgcttccg tcgaggcgcg cgacgtcgcc agaatgtcgc cgcgcaattt
     2761 gtgcagtgtg agagcgtccg ggttcgcttc aatgccttga tccagcgtct ccaatgcggg
     2821 cgtgattgcg ccgctttccc aatccgtttt cgcgcgttcg atgatgtgag gagctgatga
     2881 gccctgaagc tcctcagcga tgattgccgg tggaacgatc atccacccgc tcaaaaggaa
     2941 aagacagagg agaagcacca gacagaacaa ccggtgtcga ggatatgcac caggcatgca
     3001 gaatctcaag gattgcagta ctaataatgg cctgccttca ttatatcgca gacttccctc
     3061 gctcgactat ctgtgtcccc gagcaccgtc gtcagagctc cttggaccag aacacgaaga
     3121 acccgtgacg gcctggcaag ggtgggccac cactgtggtc gctgtaaatg ccgaaattgt
     3181 agctgtaatt gatttccact tgcgatcgcc agggcgctcc cgtcacgaca ccgccgctta
     3241 gggccggcat cgcatccatc ttgtagcgga tagatccgtc cggttggaac cgtggttgct
     3301 cgataaggcc ccaggaacct cgaatgtagg ggaacacgaa aaatatcggc tcatagcggt
     3361 actcgagatg ggctatgccg tatcgccgcg gaaacagttc gttgaaggcg acactcggta
     3421 agatgggcag ggccagtgcc tcccattcat agcccgtagg ccgaccaggc aagcgaaagg
     3481 tagaaaaccg gtccagattt tgtcccagac cggcatagac agaggcgacc agcctgtgcc
     3541 gctcgctact gacaaagggg acgcccgtgg cggcaagcgc aaacacgctt cccatgaggt
     3601 atgtccgttc gttggaaaga ccgaacacgc caccacccca cgtgctccaa tttgacctat
     3661 gcccatagat gaaatcgccg ccgaaggcat agccccgatg cgggagttcc attaaattgc
     3721 gctcgagcgc atctcggcgg actcgtgcat gcacgcgacc ttcgtaggta tcctccggga
     3781 caatgaagtt cggagacgtc ttgctcgttc ccttgaacca acgaaagccc ggttcgtacg
     3841 tcaatgacag ctccaacgcg ttgtcctggt gaccggggtc gagcagtctt cgataggcta
     3901 ggccgaatcc tccgaacaca taactccatt ccacttccac atcccgaatg atttgaccct
     3961 ccacgtactc ggatcggccg agcggaataa tcatcgtatt caacgtgaag cggaattccc
     4021 aaccggtgga tgattgagat cccacgttca tggttaaatc gttcacagga ccggaaaacg
     4081 tgcctcgaaa ccgttgcctc atatcgtctg aattgcgcca gacatacaag gctccgaacg
     4141 gtaagacttg gtaaaaactc gggccgtcag ggagatattg aatgccgaaa ctggctgccg
     4201 tgacgctctt gcgatcgcga ggcgccactt ggaccggttc accaagcact tcagtgtcga
     4261 aggcttctcc ggccagcggt gtcggtttct cttcctcggc ccacgcatgg ccggttggga
     4321 agagcatgca ggctgccagg taggctgtgc ccagcagcag acgcctcagc ctgccgttcg
     4381 ttctggccgc aaggaataac tgtctccaga ccctgtgcat atttttcagc atgacgactt
     4441 tgcgacaggg agacactagg gagggcccta gtgaagaagg gcataagggc tgcaaattaa
     4501 cccaagaatt cggtaagttc cagtgaggca cattcgattt aacaatccct ctatgggcta
     4561 gggccagcga gataaaacgg gggcgttact ggtcatgttc gacgtgatct tgtaccaacc
     4621 ggaaatccct cccaacacgg ggaacatcat ccggctctgc gctaataccg gtgtccggct
     4681 acatctggtt agaccgctcg gattttcttt ggatgaccgc cagatgcggc gcgcggggtt
     4741 ggactatcac gaatatgcga ccatgaaggt gcatgatgac tgggcgatct gtgccgcgca
     4801 tttcaaaggc agacggatct tcgcggtttc gaccaaggga tcacaacgct atgacaccaa
     4861 tcagtatctt gtagacgatg tcttcctgtt cggtcctgaa accaggggat tgccggctga
     4921 cttgctcatg caatttcctg ctgaacggcg gatccggttg ccgatgctcc cggacagtcg
     4981 tagtctcaat ctctccaacg ccgcagcggt ggtcatttac gaggcctggc ggcagattga
     5041 ttatgcggac aagcccactc ggtgccagcc gtgaattgaa agtgccatgc ccgcctgagc
     5101 aaagcggggt gagtgtccga tcatgctcta tatcagtgac cgagagtacg ggcgtccgtc
     5161 acctggtact gtcctctggc cattacagga tagacggaga aaatcggggt atgagcaggg
     5221 accaccgctg cggtcacatc cggaagcaac tgaagatgca tgttcacgaa gttaaagctc
     5281 agtccctcct gccatttctg actgccggcg ccggcatagg cagccgtttt ttcggatgca
     5341 taagttcgag tcagtaatcc ggcagcacct tctaagccat attcatgcag atccggtgcg
     5401 ccctgcacga gcacgctgac cgaactcgca tcgaaccaga gcttgatatt gcagccgacc
     5461 tgcacgtagg ggccgaggga gttggtcccc tcaaggaatg cccgaggcaa cgcgagctca
     5521 aatggcacat catttgccac cggtcgatcg acctggccta agttggtttc tctcaatggc
     5581 gctgcagaga cgaagagcaa atgcggatcc gtcatggcaa tatcaaggac ctgatcatca
     5641 tagtagctgc acagtcgtcc catgtcatag cgtagcggga atgaatatcc ggtcttggcg
     5701 taccaaatgc gcaaggcggc gtccaaagga tcttccggtg atcggctgat cttgaggtcg
     5761 aacggcaaca tgacatgcca cgcgcctgat cgcgccgcga caaacggtgc gcaggcacca
     5821 gggaactctt tatgcacggc catggtttcc ggttcgaatt ttcgcgtggc agacgtgaaa
     5881 tgctgagcaa tcttcgattc cttcgtgagt cgatatttct cctgataata ggcggccgcc
     5941 cgctgctgcg cgatggataa gtaatagtcg ggcatatccg tctcgaccac gatgcgttga
     6001 ggcagcttcc cggcagcggg gctcgcgcgt tccggtttca tggctgccga ataaagatcg
     6061 agctcggcct tcaagcgctg aatatcatgc tggagctgtc gagcgagctc ccgatcgaaa
     6121 aaactcgtag tgggaagaat gtcgagcgcc ttttgcaggc agtccaattt gtgcgtgtag
     6181 cgcttcggtt cctcggcaaa cgtttcggca gtcgcgacga acagattaaa ttgctcttcc
     6241 ggcgtgagac cgagggcgtc caagtcgtgt tctttgatga aggcgaaagc caacgggccg
     6301 aatgcctcgg ctttacggat gcacagggtc aacagaaaat tgtattgagc gcgcgcttga
     6361 tccggcgtca tggtcggcca tcataacggt ctcgccttga agggggcaag gtcaggggac
     6421 cgatcaatct cttttcagac ggtcttccaa ggccgcctcg ttcattctgt ccggctcgct
     6481 tagggatctc gccagtggaa agagggtcgg cttcaccctc atgcttcggt cccatgaaca
     6541 cgctgtggcc cattcttctc ctcgtgctgt caggctgcag gttgatcggc cttgcagttc
     6601 atcccatctc tgtcggtccc atcgaatttg atccttcaac aatcgagtct atagggatca
     6661 gtctgccggt cctcggaggc gacgaggact gtgatgccgt tgcccgcgta tcgtatcgcg
     6721 aacttggtgc cacgagttgg aaggaggcgc tcccattaca acgcgtgcga accgatctgc
     6781 tctcccgggc ggtccccagt gtatttccca tcgccgagca atttgccggc agcgtcttca
     6841 acctccggcc tgactcggcc tatgaggtca aactgacgat cgaagatccg gatggcggaa
     6901 agacgacgag gatgggcctt gtgcgtaccc gtgggatgcc tcgagacctt ccgctctcac
     6961 ctcatgttgt gcacgtcaat tcagatgata cgctggcgtc cgcccttgcc cgcgctgcgc
     7021 ccggtgaggt catcacgctc gccaagggcc actaccacgg tcccttcacg gttgatcgga
     7081 gcgggacggc tgctgatccg attatcatca aaggccacgg ccgcgaagaa accgtcattg
     7141 aatcgccggg agcggaatat ggggtcctta ttaccggttc tcacatccat gtcgaagatc
     7201 tggcgatcgg actcagcacc tgggggatca aaattgttaa cgctgaagag gtggtcattc
     7261 agcggacgca catcagagat gtgatgtacg gcatcaatgc ccgaagcggc gttaaccgaa
     7321 acgcatatat ctgcgacaat ctcttggaag gaaaaggagt cctctggccg gatacgagta
     7381 atcgaacgtg ggatttcgaa ggaattgtcg tgactggcac gggacatgtg atctgccaca
     7441 atacgcttgc ggggttcggt gatgccctgg gcctctcaca gcctacggtg attccgaatc
     7501 gcgcgatcga tttctatggc aacgacgtcg tatggggcgg cgacgacgga atcgagttgg
     7561 actatagtga acggaacgtg cgtgcgtata ggaatcggtt cgggaacgta gcaatgggaa
     7621 tcagctttca accggtctgg ggagggccgg tgtacgcgtt ccgcaacgtc atctataaca
     7681 cagcaaaggc gccctacaaa ttgaatcagg aaccgagcgg tttcttcatc gtgcacaaca
     7741 cgtccattcg ttccggctgg gcatggttgc aatatggcga actcgtctca aactttacct
     7801 tctacaacaa cctgaccatc ggcaccgaga aagccgtcta cctgacgcct tctattgaga
     7861 tcgcggacat cgactacaac ggatggagtc ctgacggaga attcaagttc gattacacat
     7921 ggaccggatt ttcctcctta catcgggagt ccccctatga gcggcatgga aggctgctca
     7981 cagcgcctgt cttcggtgag ccggtcatcc agccgccccg attcgaatcc ttcatgccac
     8041 cgccttcggc gttgaggctg catgcctcaa gtaatgccat cgatgcgggc gtgcgtctcc
     8101 ccaatatcaa tgacgattat tgcggtgagg gaccggatct cgggagtgtc gagcagggcc
     8161 gtgccgtgcc gtactatggg gtgcgtgagc cggaacatcg ccgcggcaac ggaatagggg
     8221 gccaaagaga gaccgctatt gcatgc
//
LOCUS       DASTQY010000088         3752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_344063, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000088 DASTQY010000000
VERSION     DASTQY010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344063"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..557
                     /locus_tag="VFR82_02815"
     CDS             294..557
                     /locus_tag="VFR82_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEU4500557.1"
                     /translation="MITEQTLTEYIRRAMPDATVTMIDKTGTMDHLKVMIVSDAFQGK
                     NLLDRHRLIYQALDAPMKDGRIHALELTANTKNEHEQGATGTR"
     gene            583..906
                     /locus_tag="VFR82_02820"
     CDS             583..906
                     /locus_tag="VFR82_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEU4500558.1"
                     /translation="MGDPIEEEIQKEVKAHKIVVYGKGTKQMPMCGFTRETMQFFDKY
                     GYPYEVIDVLSQPAKRDALTKMTNWPTLPKVFIDGTFYGDTDILDPMAAKGEIEPLLK
                     KAFGK"
     gene            complement(1299..3056)
                     /locus_tag="VFR82_02825"
     CDS             complement(1299..3056)
                     /locus_tag="VFR82_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500559.1"
                     /translation="MTGLPRPAVPARLCSFVIVCFGLLASAEPSHATSPVKAESPTAL
                     LLEARGIAASIDDRVERSAALDPIVVAQIAMDPPQARETLRAFPKLPNKLNYLAALAA
                     AYAKNANVAETERIYAEIVVEDQSSRVGKLAAANALGQLAMAYANKGNVEEAFRTLER
                     LKDRTKQEPPAIVGDVTASLAEAQAKQGNVHGAVKTAVSIAGENPHPLMAIVGDLVRK
                     GNAQKAQEIVAGLDDGAKSYAQWGIVQAQITQGRLTDAQVTASAIKPGHAKASALLEL
                     AHYHLQHGAKQLALLLLQEAETSARSTVNDWTRADILWRIAAETALAGDAARAIAIAK
                     SIEKEGHRDSAISDIAKAQAKRGDFADAFNTASLLKQTPPTSVLAVSRYDVTLFGILV
                     QMVKAGKGTEAKDTAAKFQDTDIRRSWLYSGIAMAYADVGNVKEAKSVLALAETEDQR
                     SARRKELRQIAEKRRLGSTPADDTRLQELVETDIEIRPGLDAIAQALARNGDLTSAMA
                     IADQLNHPAHRLDVIKTLTALHVHSGRKEQTLRWARKLSSASEKVFALVGIATALSQE
                     ADKRKGKPAPPHKSAMARG"
     gene            complement(3053..3457)
                     /locus_tag="VFR82_02830"
     CDS             complement(3053..3457)
                     /locus_tag="VFR82_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500560.1"
                     /translation="MKKEQDRQAHHRFLQALQHEHLTCTNGACGGPMEITDLTPHHAR
                     VKTYEAVCERCQQKEQIAGKEETSPPWDMASITMMAEVHLLHDQPTCPFDDTPITFIS
                     MPNPRRKARYRLLCYYCGRHTEMNWPPPEAKR"
     gene            complement(3553..>3752)
                     /locus_tag="VFR82_02835"
     CDS             complement(3553..>3752)
                     /locus_tag="VFR82_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4500561.1"
                     /translation="VNAISPGFIDSGSAPPGELAGMAKRIPAGYIGSVEDTVAAVRYL
                     LSPEARYVNGANIHISGAWGI"
BASE COUNT          789 a         1021 c         1077 g          865 t
ORIGIN      
        1 caaacgactt ctcaacaagt tctgtaccaa agttcaagga caatcgggaa atgtcctacg
       61 tttctcaatg cttcgtcatg ttgctggccg agttgtgctg gatagagaag cgacttcatg
      121 tggaatcgtt attgatccac cagtgaatcc aaacccgttg tttggcaggc catggagtcg
      181 aattgccttt ccgaaagccg ctacggtatt ctgacagcag aaaacacaag cgcacagctc
      241 agcgatgtcg gaggggacga tgggatgctg tcttaccgac gggaagaggc cctttgatta
      301 ctgaacagac actgacggag tacatcaggc gcgccatgcc ggatgcgacc gtcaccatga
      361 tcgataagac cggcacgatg gatcacttga aagtcatgat cgtatccgat gccttccagg
      421 gcaagaatct actcgatcgt catcgcctga tctatcaagc gcttgacgcc ccgatgaaag
      481 atggccgtat ccacgccctg gagctcaccg ccaacacgaa aaacgaacat gaacaggggg
      541 cgactggtac acgctgattt gactgctgga aggaggccaa acatgggaga tccgatcgag
      601 gaggaaattc agaaagaggt gaaagcccac aagattgtgg tctatggcaa gggcacgaag
      661 cagatgccga tgtgcggttt tacacgtgaa acgatgcagt ttttcgacaa gtacggctat
      721 ccatatgaag tgatcgacgt gctctcccag ccggccaagc gggacgccct cacgaaaatg
      781 accaattggc ccacgctacc gaaagtcttt atcgacggca ccttctatgg agataccgac
      841 attttggacc ccatggcggc caaaggcgag atagagccgc tgttgaagaa agcgttcgga
      901 aagtaacttc ggtcccagtc tcaccactcg aacctccggg ggtacccagt taggcctcgt
      961 ccgtgcgagg cctataaact cgtcgcgacg tcttaaacat cacaggtctc gctggtgtca
     1021 atatagagca aacggttacg ggcagcggcg aggcgcgtgc ctcccgcttc ggtaatgccc
     1081 ggcgaactcc gggccaaagt tacattccca gaaaaaaaga atcacagcaa taggtgtagg
     1141 gaaaagtaga agggcggcaa gttgggtctc gctctctgct tacggcgcaa gctgtgtctc
     1201 acccgctccg gcgatatccg gcgagcttcg ggccaatgtt acattcccag gggaaaaagc
     1261 atcacagcgg taggggaagg ggaaagcaga gaggcggctc agccccgcgc catagctgac
     1321 ttgtgtggtg gtgcaggttt ccctttccgc ttgtccgcct cttgcgagag cgcggttgcg
     1381 attcccacca aagcaaacac cttctcggaa gcgctggaca atttgcgagc ccagcgtagt
     1441 gtttgttcct tgcgccccga atgaacgtgc aatgcggtga gagtctttat tacatcgagc
     1501 ctgtgtgcgg ggtgattcaa ttgatcagcg atcgccattg cgctggtgag atcgccgttt
     1561 cgagccaacg cttgcgcgat ggcatcaagc cccggtcgga tctcaatgtc cgtttcgact
     1621 aattcttgta aacgggtgtc gtcggctgga gtactaccga gccgacgttt ttcagcgatt
     1681 tgtctgagtt cttttcttct cgcactgcgt tgatcttcgg tttcggccaa agcgagaacc
     1741 gacttggcct ctttcacatt ccccacatcg gcataggcca tcgcgatccc gctgtagagc
     1801 caagagcgtc gtatatcggt atcttgaaac ttagcggcag tgtcctttgc ttctgtcccc
     1861 ttgccagcct tcaccatttg cacaaggatc ccaaaaagcg tcacatcgta acgtgaaacc
     1921 gccaggacac ttgttggagg tgtctgcttc aacaggctgg cggtgttgaa tgcatcagcg
     1981 aagtcgcctc gctttgcctg ggccttggca atgtcggaaa tggcagagtc gcgatgtcct
     2041 tccttttcga tggatttcgc aatagcgatg gcgcgagcgg cgtcacctgc gagcgccgtt
     2101 tcggccgcga tacgccagag gatgtctgca cgcgtccaat cattgacggt cgaacgggct
     2161 gacgtttcag cttcctgtaa aagaagcaat gccagctgtt ttgccccatg ctggagatgg
     2221 tagtgagcga gctccaataa cgcactcgcc ttggcatgcc cgggcttgat tgccgaagct
     2281 gtcacctggg catcggtgag ccgaccttgt gtgatttgag cctgcacgat gccccattgc
     2341 gcgtatgact tcgctccatc gtcgagcccg gcaacgatct cctgcgcttt ttgggcgttg
     2401 ccctttcgca cgaggtctcc aacaattgcc atcagcggat ggggattttc tccggcaatg
     2461 ctcacggcgg tttttacagc gccatgcaca ttgccctgtt tggcttgtgc ttccgcgaga
     2521 cttgcagtca cgtcgccgac gatagcaggc ggctcctgct tcgtgcgatc tttgaggcgt
     2581 tcaagcgtgc gaaatgcttc ttcaacgttg cccttgttcg cataggccat ggcgagctgt
     2641 cccagcgcgt tggcagccgc cagtttgccg acccgtgaag actgatcttc gacgacgatt
     2701 tcagcataga tccgttcggt ttcagccaca tttgcatttt ttgcataggc tgcggcgagg
     2761 gcagcaaggt agttgagctt gttggggagt tttggaaacg ctctcagcgt ttctcgggcc
     2821 tgtggcgggt ccatcgcgat ctgtgcgacg acaattggat cgagcgccgc tgatcgctcc
     2881 acgcgatcgt cgatggatgc ggcgatgcct ctggcttcga gaaggagcgc tgtgggcgat
     2941 tccgccttga caggagacgt ggcatgtgag ggttcagcgg aggcaagaag gccgaagcac
     3001 acgatgacga acgagcataa tcgggcagga acagctgggc gtggcaggcc ggtcaccgtt
     3061 tggcttccgg aggaggccag ttcatttccg tgtggcgccc gcagtaatag cacagcagtc
     3121 gatatcgcgc cttgcgtcga ggattgggca tcgaaataaa tgtgatcggg gtatcgtcga
     3181 acggacacgt tggttggtca tgcagcaggt ggacttcggc catcatggta attgacgcca
     3241 tatcccaggg cggcgacgtc tcttctttcc cggcaatctg ttccttctgc tgacaccgtt
     3301 cgcacacggc ttcgtacgtc ttgacgcgag cgtgatgcgg tgtcaggtct gtgatttcca
     3361 tcggaccgcc acatgctcca ttggtgcagg tcagatgttc gtgttgcagc gcctgcaaga
     3421 atcgatggtg tgcctgccgg tcttgctctt ttttcatcct ctgtttccct ccttttagtc
     3481 gtatttaggg cagcctgcgc tgtctttgac tgcacgcgtt ccgcgagcag gaagggcagt
     3541 caggctgccc tctcatattc cccacgcacc gctaatatga atattggcgc cgttcacata
     3601 ccgggcctcg ggactcaaga ggtatcgcac agcagcgacc gtatcctcga cgctgccgat
     3661 gtaaccggcg ggaatacgtt tggccatgcc ggcaagttct ccgggaggcg cgctgccgga
     3721 atcgataaac cccggcgaga tcgcattgac gg
//
LOCUS       DASTQY010000089         3281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1552625, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000089 DASTQY010000000
VERSION     DASTQY010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552625"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..523
                     /locus_tag="VFR82_02840"
     CDS             200..523
                     /locus_tag="VFR82_02840"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4500562.1"
                     /translation="MTNHRHHFRMPVARRGLVTKGQTTALCDVIDITEQGLHFSTELS
                     LTVAETVRTECQLDENCVVHCELLITHAQPPRFGGKITHLLPEHQQQLVSFIQRLVMS
                     NMAGL"
     gene            541..1572
                     /locus_tag="VFR82_02845"
     CDS             541..1572
                     /locus_tag="VFR82_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4500563.1"
                     /translation="MGRSDREASSSQAVPILCLLGVVALLSSITPVMKYVLEHSGLTF
                     LSIANGRIVIGFLFLTVMTACWDWKGLRALSFSDGARFGALGLLGVGSYVVAAYGLLY
                     TNVTHYALIYSLLPTFTAFFSWCLSKEQVSWSGLSGILLSWAGCLAALAANLQAGTIE
                     VGLGDPLVLLFTLMMAAHIVMSMHIVRRHGVLTANTAMFGTGAVLVSGCSIAWGDPAP
                     AADLSWNVWSAVLFIGLGTAGVFLLRSRSLQSLPPATVGAYHNLIPICTIAIAHWYLD
                     EPLTGPTVIGAVAVLLGTELVRRKPPLTSLWPAVFKKGSSDIAVSVVGMVVANPRAAA
                     QSTIRHGRE"
     gene            1633..1998
                     /locus_tag="VFR82_02850"
     CDS             1633..1998
                     /locus_tag="VFR82_02850"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4500564.1"
                     /translation="MNESRPYHRGLYRLPLSYPVMYCVAATVGEGTISNLSAVGCSLE
                     TDEPLLENQHVALRLLLPDQNGSLPIDAAEVKWVKGTLAGIEFVQVDREANLRLHSFV
                     WDKMIERIQLLQQQRSGSS"
     gene            complement(1995..3017)
                     /locus_tag="VFR82_02855"
     CDS             complement(1995..3017)
                     /locus_tag="VFR82_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEU4500565.1"
                     /translation="MAISTRLTVFRFLLLGVGLLTLSFLVWHIGPGHIYEAVARFGPG
                     ALFVMLIPSVVMYVLEAYGWKIVLGPAAERISFGKILSIRTAGEVVNMTTPTAYIGGE
                     PLKAYLLQRHHIPMVEGLASVVIAKTIMTIAQVLFILIGIALAFWTLGTAGSSLQMTA
                     AGLFSAALLSCGIAAFVLVQRRGLFTWLLETFRRLGLRSTFLETRAEKLRSLDRTILG
                     FYAHHQSAFYASTGLYLLGWLAEAVEVFVIISFLGGPTSLLSAIAIGALSVLIKGGTF
                     FIPGSIGAQDGGNLLLLKAFGYSDLTGMTFALLRRFRELVWIFIGLACLAILGKKPAE
                     SQGSKF"
     gene            complement(3073..>3281)
                     /locus_tag="VFR82_02860"
     CDS             complement(3073..>3281)
                     /locus_tag="VFR82_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500566.1"
                     /translation="TGMVSVDCGDRIDGLCDSHAVDRETLWYFLETRTVILEGSLYWS
                     PRPINMHVAAQPSARVHITRGRPL"
BASE COUNT          730 a          947 c          896 g          708 t
ORIGIN      
        1 cctgtcaata ctacatgtct gccaaattac gtgtccgcca aggagatgac catgacacct
       61 ttgatgctca tgacgctaat cagcctcgcc ccctttccca tcctacttat tgccctcttg
      121 gctggacgct ggttgaaccc aaaccatcag gctgtattgt gatagcgcac gcggccaata
      181 agccggacgg agcataccga tgacgaatca ccgtcatcat tttcgtatgc cggtagcccg
      241 acggggcctt gtgacgaaag gtcagacgac ggccctgtgt gacgtcatag acatcaccga
      301 acaagggctg catttttcta cggaattgtc cctgacagtg gctgagacag ttcgcactga
      361 atgccagctg gacgaaaatt gcgttgttca ctgcgaattg ctgatcactc atgcccagcc
      421 tcctcgcttc ggcgggaaaa ttacgcatct actcccggag catcaacagc aattggtctc
      481 attcattcaa cgcttggtca tgtccaacat ggcagggttg tagcaatgtt tccttggcac
      541 atggggcgtt cagaccgtga agcatcgtcc tcgcaagccg tgcccatctt gtgcttgctc
      601 ggcgttgtgg cgttgctcag ctcgatcacg ccggtgatga agtacgtgct tgagcacagc
      661 ggcctgacct ttttgtcaat cgccaatggg agaatcgtca ttggctttct ctttctgact
      721 gtgatgacag cttgctggga ttggaagggt ttgcgtgcct tgagtttctc tgacggggcg
      781 cggtttggcg cgcttggtct gctcggcgtc ggatcttatg tcgtagctgc ttatggcctc
      841 ttatacacca acgttacaca ctatgcgctg atttatagcc tactgcccac gttcacggcc
      901 ttcttcagtt ggtgcctctc caaagagcag gtgtcatggt ccggtctctc tggcatcctt
      961 ctctcatggg cagggtgcct ggcggcgctg gccgccaatc ttcaagcagg gacaatcgag
     1021 gtgggtttgg gagacccgct cgttctcctc ttcaccctca tgatggcagc ccatattgtc
     1081 atgagtatgc acattgtcag acggcatgga gtgttgacag cgaacacggc catgttcggg
     1141 accggtgccg ttttggtatc gggctgttcg atcgcatggg gtgacccagc gccggcggcc
     1201 gatctgtcat ggaatgtttg gagtgccgtg cttttcatcg ggctgggaac agccggtgtc
     1261 tttctcttgc ggtcccgctc cctgcagtcc ttgccccctg caaccgtagg cgcctatcac
     1321 aatctcattc ctatctgtac gatcgcgatc gcacactggt atctggatga gcccctcacg
     1381 gggccaaccg tgattggagc ggtggccgta ctcctgggca ctgagctcgt ccgccgcaag
     1441 ccgccgttga ccagcctgtg gcctgcggtc ttcaagaaag gaagcagtga catagccgtc
     1501 tcggtggtcg gaatggtggt agccaatccg cgtgcggccg ctcaaagcac tatccggcat
     1561 ggtcgtgaat gatttcgccc ctctaaggag ggatacagtg ggccatggac aacacagtag
     1621 actcacgtca cgatgaatga gagcaggccc tatcatcgtg gcctctatcg tctgccgctg
     1681 agctaccctg tcatgtattg tgtggcagcc accgtcgggg aaggtacgat atccaacctg
     1741 tcggcggtgg ggtgctcact tgaaacggat gaaccgctgt tggagaatca acacgtggca
     1801 ctccgactcc ttcttcctga tcaaaacgga tccctgccca tcgacgcagc cgaagtcaaa
     1861 tgggtcaaag gcacacttgc cggcatcgaa ttcgtccaag tggaccgcga agcgaacttg
     1921 cgcttgcatt cgtttgtctg ggacaagatg atcgagcgga ttcagctcct gcagcagcaa
     1981 cgctcaggat cgtcctagaa cttggatccc tgactctcag ccggcttctt tcccaggatc
     2041 gccaagcaag ccaggccgat aaagatccag actaattccc tgaaacgccg caaaagcgcg
     2101 aaggtcattc ccgtcaagtc tgagtacccg aaggccttca gtaagagcaa attacctcca
     2161 tcctgggcac ctatactccc cgggatgaag aatgtcccgc ctttgatcag gacggataac
     2221 gccccgatcg caatggcaga caggagactt gttggcccac cgagaaagga aatgatcacg
     2281 aagacttcca ctgcttcagc gagccacccc agcagataca ggccagtcga agcataaaac
     2341 gccgattgat gatgggcgta aaaacccaga atcgtcctgt cgagcgaccg gagtttctcg
     2401 gcgcgcgttt caagaaaggt actgcgaagg ccaagcctac gaaatgtctc cagcaaccac
     2461 gtaaacagtc cgcgtctttg caccaggacg aacgcggcaa tcccacacga caataaggcc
     2521 gcgctgaaca gtcccgcggc agtcatttgg agggacgatc cagctgtccc cagcgtccag
     2581 aaggccaacg caattccaat aaggatgaag aggacctggg cgatcgtcat aatcgttttg
     2641 gcaatcacga ccgaagcgag gccctcgacc atcggaatgt ggtgcctttg aaggagatag
     2701 gccttcagcg gctccccgcc gatgtaggca gtaggcgtcg tcatattcac tacttcaccg
     2761 gccgtccgga ttgagaggat tttgccaaag gaaatgcgct ccgccgctgg gcccaggaca
     2821 attttccacc cataggcttc gagcacatac atgacgacag atggaatgag catcacgaag
     2881 agggcaccgg gcccgaaccg agcgactgcc tcataaatgt ggcccgggcc tatatgccac
     2941 acaagaaagg acagtgtgag caggccgacg cccagtagaa gaaatcggaa cacggttagg
     3001 cgggtggaaa tggccacggg ccctcatctg ccgtgaccag aaacttggaa accccgagac
     3061 cacggtccgc actcacagcg gccttcctcg cgtgatgtgc acgcgtgcag agggctgggc
     3121 cgcaacgtgc atgttgatcg gacggggcga ccaatacaag ctgccctcca gtatcaccgt
     3181 acgggtctcg agaaaatacc agagggtctc acgatccaca gcatgactgt cgcaaaggcc
     3241 atcgatccga tcgccgcaat ccacagaaac cattccagtt t
//
LOCUS       DASTQY010000090         8306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1753957, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000090 DASTQY010000000
VERSION     DASTQY010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753957"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFR82_02865"
     CDS             <1..399
                     /locus_tag="VFR82_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500567.1"
                     /translation="EPVSFIRRRSVRLIIYGGLIAFVVIMILVPLIAKLRDPNFEKHI
                     AEHRVMVGMSKEQVLQSWGGPQTINTSFTKDGIRQEEWIFEDWESAAVVKHRYLYFEE
                     GTLIGGWFQGSGERLPTQSPSNPHNPKQTG"
     gene            complement(402..644)
                     /locus_tag="VFR82_02870"
     CDS             complement(402..644)
                     /locus_tag="VFR82_02870"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEU4500568.1"
                     /translation="MNLVRLTEPYNVGELALLQSLLEGSGIAYLIRHANVSSLYPGVL
                     GLNSHVMVEERDYPRAEAILQRLRVDVREVTGDIGG"
     gene            complement(736..1020)
                     /locus_tag="VFR82_02875"
     CDS             complement(736..1020)
                     /locus_tag="VFR82_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500569.1"
                     /translation="MKQVTVQNPDEILSLLADVALRGSGFVTECLLDYVLDEGFTEPI
                     YLNASGEDPDAYFKGQSPAWAVYQVREWKRVLTISGGPGKQRRVQITETP"
     gene            1074..1823
                     /locus_tag="VFR82_02880"
     CDS             1074..1823
                     /locus_tag="VFR82_02880"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEU4500570.1"
                     /translation="MTRPQTNSRFQEELLRIMDRKHHWAWPSFADGTVSVEQLTCHFQ
                     QEYAVYVRDFPVMLARIHGHNPPAAVRRMLAENIYEEDTGGLSLGKSHPELFLQMMAG
                     LGLPAQSFDRITLLPAARRYRAWLDRASYHRQWIVGAAALTVFVEGSVKDRKELDEPS
                     KPKTAEEIESIVQQHPLVRHYGLSPDSMDLIRSHQMVEAGHRHDAYKMVVSYATTRAQ
                     QQAVLSCLKTCLRLWSAYRDGVARACRLRKP"
     gene            1912..2709
                     /locus_tag="VFR82_02885"
     CDS             1912..2709
                     /locus_tag="VFR82_02885"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU4500571.1"
                     /translation="MVLIPAGEFTMGTPAGTDGLADEHPQRSVFLSSFYIDRYELSNA
                     KYFEFVSATRHRMPENSNTASTLWTNGKPMSGIEQHPVVNVSWTDAEAYCRWAGKRLP
                     TEAEWEKAARGTDGRRYPWGNEWNISMANSASFWAGRTIEFDSGADWDAFWVKGEGAQ
                     IAKSKGLKGEVLTLPVGSFPDNVSPYGLFDMAGNVAEWVQDWYDPNYYNAAPLDNPVG
                     PERGAIKSMRGGSWLKPAVSLRTSDRDWGIMDSRPSGTGFRCAMDAY"
     gene            complement(2730..3395)
                     /locus_tag="VFR82_02890"
     CDS             complement(2730..3395)
                     /locus_tag="VFR82_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500572.1"
                     /translation="MRVPFHILALAGLVLMPAGAWAGAIGEAGMPQRLSLEKLESTLP
                     PGTRLMVHPAAIERFLEELDGAPPDWASLYGHGHQDPGHDDRLFALNRERDARREGHE
                     ALETAVAFLWTGELSRYDPGSDGFPVALGPKLVKTAWGMVRFKPEDAPANLSVTTDVI
                     RREHWRRQIAEGHPVEIDVLMTGKLIPEESIVYDFSHDEEGLGLIMPFVRVERVDFLL
                     IEP"
     gene            complement(3405..3725)
                     /locus_tag="VFR82_02895"
     CDS             complement(3405..3725)
                     /locus_tag="VFR82_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500573.1"
                     /translation="MSDVDLLLQRSIADISQSDPLIKLLEQVRIGRMQANDPGLRAIT
                     ESWLAAYRKAIDSPGLTRQSLLRIDPAPRLTVLMEQGMLPPNHEGARKLLADFTQALS
                     RASS"
     gene            4028..4300
                     /locus_tag="VFR82_02900"
     CDS             4028..4300
                     /locus_tag="VFR82_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500574.1"
                     /translation="MYQEEKTFTLRFSLEASFPDDYDGEDDRHVWIHEWESQIKPQVL
                     KQVFESLRQHPAWSSHVRNRGIAPTDEIEIVVAKDFSKPVPFSLKS"
     gene            4297..5430
                     /gene="hemW"
                     /locus_tag="VFR82_02905"
     CDS             4297..5430
                     /gene="hemW"
                     /locus_tag="VFR82_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEU4500575.1"
                     /translation="MTPALGLYLHIPFCRQRCDYCAFYLEVHREPAAEAFCRALETEI
                     ALYAAEPSLKGRQFASLYFGGGTPTVLKPEQLTGLLAAVQRSFLLRPDCEITVEAHPG
                     TVTTRDLAALRDIGVTRISFGAESLADDELASLGRPGAQAETIEAVMSARSAGFIDIN
                     LDLMYGLPGQSLESWERTLRRCIQLSPTHVSCYALTVEEGTRLAHEVRRAQRQQPDEL
                     LQIEMDGLTQGILGQSGYEQYEISNYARSGFQCRHNLLYWTQGEYLGVGPSAQSLVAN
                     VRFGNVANLTSYKVALTENRLPVQERTLLTTQEQLRDAVIFGLRLRQGIPTSQLKAHV
                     ENYGYLGIWRELCTDQLIEEAGGRSRLSARGRIFADTIAERLF"
     gene            complement(5483..5860)
                     /locus_tag="VFR82_02910"
     CDS             complement(5483..5860)
                     /locus_tag="VFR82_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500576.1"
                     /translation="MLQSAPMLDAKVWWPLFPLLLLLVIVCLTWALVIAVRGHADARA
                     KWLQFGAFACYVLAAVSAVASERGAVSANLHRPFSLATQLCLLLALVHHWKSPQRPLR
                     WLNAAAWGGILADTALHVLMVRH"
     gene            5934..6131
                     /locus_tag="VFR82_02915"
     CDS             5934..6131
                     /locus_tag="VFR82_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500577.1"
                     /translation="MPVNMDPAKKRRKQAPTAQVPSHEHSPASRKSFGEETEEDRIKA
                     LADAEKKNLWDATEVIDMDTF"
     gene            complement(6154..7230)
                     /gene="cax"
                     /locus_tag="VFR82_02920"
     CDS             complement(6154..7230)
                     /gene="cax"
                     /locus_tag="VFR82_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEU4500578.1"
                     /translation="MKTSFSMSWLDLLLVFVPVTLVLAFIQPGHLGVFISAALAIIPL
                     AGLLGRATEHLTAHVGAGIGGLLNASLGNAAELIIALVALRGGLHDVVKASLTGSIIG
                     NILLVLGASMLAGGLKYERQKFNQTAAGMGASLLLLAAVGLIIPALFHLTAADRGIAV
                     ERELSLEISIVLFAIYVMSLVFSLRTHRHVSSGSWRETKHVGDRSWSRGRAVTVLAIV
                     TVLIALMSELLIGALDHAAKTLGLTEIFVGVILVALVGNAAEHSTAVMVAMKNKMDLA
                     LSIAVGSSIQIALLVAPVLVFASYLFGAPLDLIFTPFEVAAVTISVLIVGFVSLDGES
                     HWMEGVMLVGVYVMLAIAFYFLPA"
     gene            7365..7661
                     /locus_tag="VFR82_02925"
     CDS             7365..7661
                     /locus_tag="VFR82_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEU4500579.1"
                     /translation="MAESPTPVATPLTTETPSTGTGEGLEARVVVYNCDCHTYQQVIA
                     LFCQCIPGMTPSKAFELAWKIDHQGEAVVFTGEWKQAEEVAGKLGGGGLRVAVQ"
     gene            complement(7669..8001)
                     /locus_tag="VFR82_02930"
     CDS             complement(7669..8001)
                     /locus_tag="VFR82_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500580.1"
                     /translation="MERRAASHTEEEEMNQRRRLLNAAKKGDQKAVAKLFELYQVKVY
                     NNEMVSKLNRTAGTHKLQAEVAQAKAAAKGGGSSRKSMPKKSVPSSSRHHGKARPTRS
                     DKKVHRKK"
BASE COUNT         1914 a         2407 c         2297 g         1688 t
ORIGIN      
        1 gaaccggtct catttatccg gcgcagatcc gtccgcctga tcatttatgg agggttgatc
       61 gcatttgtcg tcatcatgat tctcgtacca ctcatagcaa aattgcgcga tccgaatttt
      121 gagaaacaca ttgccgaaca ccgggtcatg gtgggcatgt cgaaagagca agtgctgcaa
      181 tcatggggtg gacctcaaac aatcaacacc agctttacaa aggatgggat ccggcaggaa
      241 gaatggatat tcgaagattg ggaaagcgcg gcggtcgtca agcaccggta tctgtatttc
      301 gaggaaggga cgttgatcgg aggatggttt cagggctcgg gcgaacggtt gccgacacaa
      361 tcaccctcga acccccacaa tccgaagcag accggctaaa gttatccgcc aatgtcgccg
      421 gtcacttcac gcacatcgac tctcaagcgc tgcagaatcg cctcggctcg cgggtaatcc
      481 cgttcctcca ccatgacatg agaattcaat cccagcacgc ccggatagag actactgacg
      541 ttggcatgac gaatcaaata cgcgatcccg cttccttcga gcagactttg aagcagggca
      601 agttctccga cattataggg ttcggttaga cgaaccagat tcatgtcaac catccttggt
      661 acatgattcc tgtatccaag agcatatcac cggatcgttg ctttttcgac gcgacactcc
      721 tacactggtt cgagactatg gtgtctcagt gatctggacc cgccgctgct tgccgggacc
      781 tcccgaaata gtcagcacgc gtttccattc ccgaacctga tacactgccc aggccggtga
      841 ctgtcccttg aaataggcat ccggatcttc tccgctggca ttcaggtaaa tcggctcagt
      901 aaatccctcg tccaagacgt agtcgagaag acattcggtg acgaaaccag atccgcgcaa
      961 cgcaacgtct gccagcagcg acaggatttc atcggggttc tgaacggtca cttgtttcac
     1021 ggtgtctcca tccggcgaat tgtaacgagg cctcgacgag gaaggcaacc cgcatgaccc
     1081 gaccgcagac caacagccgc tttcaagaag aacttctgcg aatcatggac cgcaagcacc
     1141 actgggcctg gccatcgttc gccgatggga cagtctcggt cgagcaattg acatgccatt
     1201 ttcagcagga gtacgcggtc tacgtgcgcg actttccggt aatgctcgca cggattcatg
     1261 gccataaccc gccggccgcg gttcggcgga tgctggccga gaacatttat gaagaagaca
     1321 ccggagggct ttcactcggg aaatctcacc cggaactgtt tcttcagatg atggctggat
     1381 tgggcctccc cgcccagtcc tttgaccgga tcaccctcct ccccgccgca cgtcgataca
     1441 gggcctggct cgatcgtgct tcctatcatc ggcagtggat cgttggtgcc gccgcgctga
     1501 ccgttttcgt ggaaggcagc gttaaggacc gtaaagagct ggacgagcct tccaaaccca
     1561 agacggccga agaaatcgag tcgatcgtcc aacaacaccc gctggtccgc cactatggcc
     1621 tctctcctga ttccatggat ctcattcgtt ctcatcagat ggtcgaggcc gggcatcgac
     1681 atgacgccta caagatggtc gtgagctacg cgacgacccg tgcgcaacaa caagcggtcc
     1741 ttagctgtct gaagacctgc ctgagattgt ggagtgccta ccgcgacggg gtagcgcgtg
     1801 cctgtcgtct tagaaagccc tgacgggcgg caaagtgccg gctggtttat tctctgctgt
     1861 gtcacggtcc tgatgtgccc tggatggagc ttcggctcct tagatagcac catggtgttg
     1921 atccctgctg gtgaatttac gatggggacg ccggccggca ccgatggact cgccgacgag
     1981 cacccccagc gttctgtgtt tctttcctcc ttttacatag accgctatga actttcgaat
     2041 gccaagtatt tcgaattcgt ctccgcgaca cgccaccgaa tgcccgaaaa ctccaacacc
     2101 gcctcaactt tatggactaa cgggaagccg atgtccggga ttgaacagca cccggtagtg
     2161 aatgtcagct ggactgatgc ggaggcctat tgtcgatggg ctggcaagcg ccttccgact
     2221 gaagcggaat gggaaaaagc cgcgagagga acggatggcc gtcgatatcc ctggggcaac
     2281 gagtggaaca tttcgatggc gaacagcgcc agcttttggg ctgggcgcac aatcgagttt
     2341 gacagcggag cggattggga cgccttctgg gtcaagggtg aaggtgccca gatcgcgaag
     2401 agcaaaggat tgaaaggaga agtgctgacg ctgccggttg gcagtttccc cgacaacgtc
     2461 agtccgtatg gtctcttcga tatggcgggc aatgtcgccg aatgggtgca ggattggtac
     2521 gatcccaact attacaatgc cgccccgctg gacaaccccg ttggtccaga acgtggcgcc
     2581 attaaatcca tgcgtggagg ctcatggctc aagccagcgg taagcctacg cacaagcgat
     2641 cgcgactggg gcatcatgga cagccgcccg agtggaacgg gttttcgctg cgcaatggac
     2701 gcctactagc tccgcccaag ctgggcggac taaggttcta tcaataagaa atccacccgc
     2761 tcgacgcgca cgaagggcat gatcagcccc aagccctctt catcgtgaga aaaatcatag
     2821 acaattgatt cttcaggtat caacttcccg gtcatcagga catcgatctc gaccgggtga
     2881 ccctccgcga tttgacgccg ccagtgctca cgccggatca cgtcggtcgt gacgctcaga
     2941 ttcgctggcg cgtcttcagg cttgaaacgg accatccccc aggcagtttt gaccagcttc
     3001 ggtccaagcg cgacgggaaa accgtcgctc cccggatcat agcgggatag ttcgcccgtc
     3061 cagaggaaag ccaccgccgt ttccagcgcc tcatggcctt cacgtcgcgc atcccgctcc
     3121 ctgttgaggg caaaaagccg gtcatcatga ccggggtcct ggtgcccgtg accgtacaga
     3181 gacgcccaat cagggggcgc accgtccagt tcctccaaaa atctctcgat ggcagcggga
     3241 tggaccatga gccgggtgcc gggaggaagg gtgctctcaa gtttctcgag ggaaagccgc
     3301 tgaggcatgc cggcttcgcc gatggcaccg gcccatgctc cagccggcat gagcactaac
     3361 cccgccaacg cgagtatgtg aaacggaacc ctcatcggca tcactcagga cgacgcacga
     3421 ctcagggctt gcgtgaaatc ggcaagcagc ttcctagccc cctcatggtt aggaggcaac
     3481 atcccttgct ccatgagcac ggtcaatcga ggcgcaggat cgatgcgaag caacgactgt
     3541 cgtgttaatc cgggtgaatc gatggctttg cggtaggccg caagccagga ttccgtgatg
     3601 gcacgcaggc cgggatcgtt agcctgcata cggccaatcc gtacttgttc aagaagtttg
     3661 atgaggggat cggattgcga gatgtccgca atagaccgct ggagcaacag atcgacgtcg
     3721 ctcattgcta attcatcgag caggctcccg gtcctctggg atctccgcag ctgccacagg
     3781 cgcctcctcc accggccggt gaggcccaac cacccgttgc gccaacgcca aatgaagaaa
     3841 actgtttgtc gagcgcggtg gtgcgacact ccggacaggc ggcctcgacc gacccctgaa
     3901 ccaggagttc gaagcgatga ttgcattcac ggcagacata ttcgaagata ggcataatcg
     3961 tgattctcct tggttgaacc cattatagaa gtgtcgcgtc acgcttcgca acgcatgagg
     4021 cttggatatg taccaagaag aaaaaacctt caccctgcgc ttcagtctgg aggcctcctt
     4081 ccctgacgac tacgacggcg aagatgatcg ccatgtctgg atccatgagt gggagtctca
     4141 gatcaaacca caagttttaa aacaggtttt cgaatcactt aggcagcacc cggcctggtc
     4201 atcccatgtt cgaaaccggg gcattgcacc cacggacgaa atcgagatcg tcgtcgcaaa
     4261 ggatttttcc aaaccggtcc cgttctcgct gaagtcatga cgcctgctct tggcttgtac
     4321 ctccatatcc ctttttgccg gcagcggtgt gattactgcg ctttttacct ggaggtgcac
     4381 cgtgagccgg ctgcagaagc cttttgccgt gcgctcgaaa ccgaaattgc cctgtatgcg
     4441 gctgaaccat ccctcaaagg ccgccagttc gcctctctct actttggcgg tggaactccc
     4501 acggtcttga aacctgaaca gttgacaggg ttgctcgccg ccgtgcaacg ctcgtttctc
     4561 ctgcgtccgg attgtgaaat caccgtagag gcgcatccgg gaacggtcac gacacgcgac
     4621 cttgccgcct tacgggacat cggtgtgaca aggatcagct ttggcgcgga atccttagct
     4681 gatgatgagc tggcgagcct tggccgtcca ggggctcagg ccgaaacgat cgaggcagtc
     4741 atgagcgccc ggtcagcagg atttatcgac atcaatctgg atctcatgta cgggcttcct
     4801 ggacagtcat tggaaagttg ggaacggaca ctccgtcgct gcattcagct gagcccaacg
     4861 cacgtctctt gttacgcctt gacggtggaa gagggaacca gactcgctca cgaggtgcgc
     4921 cgagcgcagc ggcaacagcc tgatgaattg ttgcaaattg aaatggatgg tttgacgcaa
     4981 ggcatactgg gccaatccgg ctatgaacaa tacgaaattt ccaactatgc caggtccggt
     5041 tttcaatgcc gccataatct cttgtactgg acacagggag agtacctggg cgtcgggcct
     5101 agcgcccaat cgcttgtagc aaacgttcgt tttggcaatg tggccaacct cacgagctac
     5161 aaggtcgcgt tgacggaaaa ccgtctcccg gtgcaggagc ggacgctact gaccactcaa
     5221 gagcagctcc gtgacgcggt catctttggg ctgcggctcc gccaaggtat cccgacttcc
     5281 caattgaaag ctcatgtcga aaattatggc tatttgggaa tctggcgtga gctctgcaca
     5341 gaccagttga tcgaggaggc cggcggtcgc agccgactat cggcaagagg tcgcatattc
     5401 gccgatacaa tcgctgaacg cttattttag tggaacggcg gcatggtggc ttgccaacac
     5461 ggattgaaac ccgctgccag tttcaatgtc tgaccatcag gacgtgcagc gccgtatcgg
     5521 ccaggatgcc tccccaggcc gcggcattca accagcgtaa tgggcgttgc ggagacttcc
     5581 agtggtgaac caaggcaagc agcaggcaga gttgcgtcgc caacgaaaag gggcgatgca
     5641 gattggctga gacagcccca cgttcgctgg ccacagccga caccgcggcg agcacgtagc
     5701 acgcaaaggc gccgaattgc agccactttg cccgagcgtc agcgtgaccc ctgaccgcga
     5761 tgaccagcgc ccacgtcaag cagacgatga caagtaacaa caacagcggg aagagcggcc
     5821 accacacctt ggcgtccaac atgggcgccg actgtaacaa gcctctccca ggaggtcaac
     5881 gacagctcag ccattgactt ttttcattgg cgggagagag actaacgcca cctatgccag
     5941 tgaatatgga ccctgccaaa aaacgtcgta aacaagcgcc caccgctcag gttccatccc
     6001 atgagcactc ccccgcttcg cgcaaatctt tcggcgagga gacggaggag gatcggatca
     6061 aagcgctcgc agatgccgaa aagaagaacc tgtgggacgc gacagaagtc atcgacatgg
     6121 ataccttcta aaaatatgtg gcttcttgtc gtttcaggcg ggaagaaagt aaaaggcaat
     6181 cgctagcatc acatacactc cgaccagcat cacgccttcc atccagtgcg attcgccgtc
     6241 caacgaaaca aagccgacaa tcaagactga aatcgtgacc gcagcgacct cgaacggtgt
     6301 aaaaatcaaa tccaacggcg caccgaagag ataactcgca aagaccaaga ccggcgcgac
     6361 gagcaaggcg atttgaatgc tcgatccaac cgcaatgctc agagccaggt ccatcttgtt
     6421 tttcatggcg accatgacag cggtcgagtg ttccgcggcg tttccgacga gcgcgacgag
     6481 aatgacgccg acaaatattt ccgtcaaccc gagcgttttt gcggcatgat ccaacgcccc
     6541 gatcagcagt tcactcatga gggcaatcaa cacggtaacg attgccagca ccgtcacagc
     6601 tcttccgcgg ctccacgacc tgtctccgac gtgcttggtc tcccgccagc tacccgaaga
     6661 gacatgacga tgagtcctca acgaaaacac gaggctcatc acgtaaatgg cgaataagac
     6721 gatggatatt tcaaggctga gctcgcgttc tacggcgatg ccccggtccg ctgccgtgag
     6781 atggaacaga gcgggaatta tcagtcccac agcggcaagc aagaggaggc tcgcacccat
     6841 tcccgctgcc gtttgattga acttttgccg ttcgtacttc agtccgcctg cgagcatcga
     6901 tgcacccagg accagcagaa tgttgccgat gatcgatccg gtcagggaag ctttcacaac
     6961 gtcgtgcaag cctccgcgta acgccaccag cgcaatgatc aactctgccg catttccgag
     7021 cgaagcgttc aagaggccgc cgattccagc tccaacatga gccgtcaaat gttccgtggc
     7081 tcgccccagg agaccggcga gcgggatgat ggcaagcgcc gcggaaataa ataccccaag
     7141 atggccgggt tgaatgaacg cgagcacgag ggtgaccgga acaaaaacca acaagagatc
     7201 gagccaagac attgaaaacg atgttttcat cgcggcgcac gttaacatga gcgggcgcca
     7261 ttgtcgacga acgggcgctc cgcggccgtc cgaatgccac accggctgcg cacacggaaa
     7321 aggatcttaa tcgcacaaga gaactctgtt agaatcgcca ctccatggcc gaatccccca
     7381 ctcctgttgc gacaccgctg accacagaaa ctccctcgac ggggactggc gaggggttag
     7441 aagcccgcgt ggtcgtctat aactgcgatt gtcatacgta ccagcaagtc atcgcacttt
     7501 tttgtcagtg catcccggga atgacgcctt ccaaggcgtt cgagctcgca tggaagatcg
     7561 accaccaagg agaggcggtg gtcttcacgg gagagtggaa acaagcggag gaggtagcgg
     7621 ggaagctagg ggggggcgga ttacgtgtgg ccgttcagta gccagccctt attttttcct
     7681 gtggactttc ttgtcggagc gggttggtcg tgctttccca tgatgtctgc tcgacgaggg
     7741 gacggatttt tttggcatcg actttcgaga cgatcctcca ccctttgccg cggccttggc
     7801 ctgcgcgacc tcagcctgta gcttgtgcgt gccagccgtc cggttgagtt tgctcaccat
     7861 ctcgttattg tagaccttta cctggtacaa ctcgaaaagt tttgcgaccg ccttttgatc
     7921 gccttttttg gcggcgttca aaagccggcg tcgctgattc atctcttctt cttcggtatg
     7981 agaagcggcc cgtcgttcca tgccatcctc gttcgaatgt ccgatgcaat cgcctctgca
     8041 gcggcgaagg agtaaaccgc cgaactatat cagaagcaag ggccttttga aaatgccggt
     8101 gccatgaccg ccagggattt ccctccattg agcgactcaa aacggggcag ttataatgtc
     8161 gaacactttt cccacatgga gggacatcat ggacgctcac aagccggatc aaccgaccgt
     8221 agccgacgac cctaaagcgc gtgaagtgct gcgccgggcg tttgaaaaca ccgccaggtg
     8281 gcccaaggac ttcaaaggct tcactg
//
LOCUS       DASTQY010000091         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1551685, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000091 DASTQY010000000
VERSION     DASTQY010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551685"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..734)
                     /locus_tag="VFR82_02935"
     CDS             complement(144..734)
                     /locus_tag="VFR82_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU4500581.1"
                     /translation="MGRVYTIVLGVLMTSGFWGCGYNDLQGLDEDTKAAWSEVVNQYQ
                     RRADLIPNLVATVKGYAAHEKETLEGVVQARAQATGIQVTPEMLKDPAAFEQFQKAQA
                     GLTSALGRLIAIAENYPNLKADQNFRDLQSQLEGTENRITVARKRYIDRVAEYNKMVR
                     YFPTNLTAKFILHLEERPNFAVADEKAVAKPPEVKF"
     gene            811..1116
                     /locus_tag="VFR82_02940"
     CDS             811..1116
                     /locus_tag="VFR82_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500582.1"
                     /translation="MSLERASNQPGGFQLRHRLLGIYQGSAIELAFWHPSSGMPEYGL
                     CRFSTEDKAQALADFLTSPACPEPFDPKDLTIEPYDLAEHERLTTDYPLPSAWEQPT"
     gene            complement(1174..1695)
                     /locus_tag="VFR82_02945"
     CDS             complement(1174..1695)
                     /locus_tag="VFR82_02945"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4500583.1"
                     /translation="MGRSLNCPQCRREQVLRARPLSASERVAAIFLLSPFSCQHCSCR
                     FLASRIGLDRPRHPIDRREHLRIPVRLYLSFSGGKVRGEGTVLDLSMGGCIIKSETQV
                     RVDDIFYLEIATGSQEQPLEVAAMVRSVSPRGIAFKFLRAAQENKRLLAFIQSQTADH
                     MQKSTAMAVPAKS"
     gene            complement(1879..>2428)
                     /locus_tag="VFR82_02950"
     CDS             complement(1879..>2428)
                     /locus_tag="VFR82_02950"
                     /inference="COORDINATES: protein motif:HMM:NF005746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEU4500584.1"
                     /translation="TVCQLYMRRFPDGATITIEPWRAKAFPIIKDLVVDRSAFDRIMQ
                     AGGYISVNTGGAVDGNVLLIGKDRAETAMDAASCISCGACVAACKNASATLFVAAKVS
                     HLALLPQGQPERTKRAKAMVAAMDREGFGSCGNQYECEAVCPKSISVRFIAKLNREFL
                     RATIEEPSPLAEQPTDPHDESH"
BASE COUNT          501 a          711 c          710 g          506 t
ORIGIN      
        1 gatccacaat gcgacccgtc aacggaggga cgtccagcgc cgagatgagc agcggattca
       61 gcagaagtcc cacgaagacg acgagccgcg ccggctctcg ccagacacgg ctcatacaga
      121 ggatagacat catgcaggca agtctagaac ttgacctccg gcggcttggc gacggctttt
      181 tcatctgcca cggcgaagtt gggccgctct tccaggtgga ggatgaattt ggcggtcaag
      241 ttggtcggaa aataccgcac catcttgttg tattccgcta cgcgatcgat gtagcgcttg
      301 cgagctacag tgatccggtt ttcggtgcct tccagctgac tttggagatc gcgaaaattc
      361 tgatcggctt ttaggttggg atagttttcc gcaatggcaa tgagacgtcc gagggcagac
      421 gtcagaccgg cctgcgcttt ttgaaactgc tcgaacgctg ccggatcctt cagcatctcc
      481 ggggtgactt gaatgccggt ggcttgcgcc ctcgcctgaa ccacgccttc gagtgtctct
      541 ttttcatgcg cggcgtatcc tttcaccgtc gccacaagat tgggaatgag gtcggccctc
      601 ctctggtatt ggttgaccac ttcgctccag gccgccttgg tgtcttcgtc gagtccttgt
      661 agatcgttat agccgcaccc ccagaaacca ctagtcatga gaacgcccag gacgatcgtg
      721 tacaccctac ccatacctct cctccttgtg atgaacgtgc aaacggatca tgctatcatt
      781 ggtcgaacac ggaagatagc gggagactcc atgtcgctgg aacgcgcatc gaatcaaccg
      841 ggaggatttc agctcaggca ccgtttgctg ggcatctatc agggaagtgc gattgaactg
      901 gccttctggc atccttcatc cggcatgccc gaatatgggt tgtgccggtt ctcaaccgag
      961 gacaaggcac aggcgctcgc tgatttcttg acatccccgg cgtgcccaga accgtttgat
     1021 cccaaggacc tgacgatcga accgtatgac ctcgccgaac atgaacggct gacgactgac
     1081 tatcccctgc cctctgcctg ggaacaaccg acataaagcc gtgccgattg ccgcctgcga
     1141 gaatttgagc actgatgtgg agaagaaggc gtgctagctt ttggccggga cagccatggc
     1201 tgttgatttc tgcatatggt cggcggtctg cgactgaatg aaggcgagca ggcgcttgtt
     1261 ctcctgggca gcccgcaaaa acttgaaggc gatgccgcgg gggctcaccg agcgtaccat
     1321 cgcggccact tccagcggct gctcctgtga tcccgtggcg atctccaagt agaagatatc
     1381 gtcgacccgc acttgtgtct cgcttttgat gatgcagccg cccatcgaca gatcgagcac
     1441 cgtgccctca ccgcggacct tcccaccgga aaacgagaga tacagccgca cgggaatccg
     1501 gagatgctca cgacggtcga tcggatggcg cgggcggtcc aatccgatac gggaggccag
     1561 aaagcggcaa ctgcagtgct ggcaggagaa gggggacaac agaaagatcg ccgccacccg
     1621 ttcgctcgcc gagagcggcc tggcgcgcag cacctgttca cggcggcatt ggggacaatt
     1681 cagcgagcga cccataaaaa ttgtttgagt caggacctgt cgcagcaacg gtgatatctc
     1741 actcactcat cgatagtgca tgtcccccga tctgtcaagc gaagaggcgt aaacagccac
     1801 gcaagccact tagcgcagcg ctccatagct cttgggccat ctgctggatc ggcacggagg
     1861 gtgagccgcc aggtcgtatc agtgcgattc gtcgtgagga tccgtcggct gttccgcgag
     1921 gggactcggc tcttcgatgg tggctcggag aaattcccgg ttgagcttcg cgatgaatcg
     1981 tacgctgatc gactttgggc agaccgcttc gcactcgtat tggttgccgc aggacccgaa
     2041 cccttcgcga tccatggcgg ccaccatggc cttggcccgt ttcgtccgct ccggctgacc
     2101 ttgcggtaac aaagcaaggt gcgagacttt ggcggccacg aacagcgtgg ctgacgcatt
     2161 cttgcacgca gcgacgcacg cgccacatga aatgcaagag gcggcatcca tggccgtttc
     2221 ggcccggtcc ttcccgatca acaacacgtt tccgtccacg gctccgccgg tattgacgga
     2281 gatatacccc ccggcttgca tgatgcggtc aaatgcgctt cgatccacga cgagatcctt
     2341 gatgatcgga aaggcctttg cgcgccaagg ttctatggtg atcgtggcgc cgtcgggaaa
     2401 tcgacgcatg tagagttggc agacggtg
//
LOCUS       DASTQY010000092         7870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1147827, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000092 DASTQY010000000
VERSION     DASTQY010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147827"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VFR82_02955"
     CDS             <1..334
                     /locus_tag="VFR82_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500585.1"
                     /translation="DQQVGFIKDALKFSYLLLRTDGRTIAERSPHTFRMVSELRKLKG
                     DTRAWLCNERGRCEVGRLDRAETDMNSRWDECQRGTIVRIEHITRKDGASLGRISDRS
                     AVQIVRSV"
     gene            354..1016
                     /locus_tag="VFR82_02960"
     CDS             354..1016
                     /locus_tag="VFR82_02960"
                     /inference="COORDINATES: protein motif:HMM:NF016704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU4500586.1"
                     /translation="MSFITPDYFIHAANILLLIAYSVRDVLWLRLFAVAASLIAIPFY
                     LFQPTVLWAPLAWSVVFAAINLLQSWLLFVERRPVKLTAEEEEVGRLALQDLPPRKIL
                     QVLSIGSWITAEVGEHLIERGKLPDSVSLIVRGKVRITKDGRMLRDLIPGDIVGSALI
                     LNGIPSNVDAVTVEPLRAMRWHIGTLQKYLSAHPETRTVMQRHLARDLAGKIGRLVTE
                     PS"
     gene            complement(1492..1674)
                     /locus_tag="VFR82_02965"
     CDS             complement(1492..1674)
                     /locus_tag="VFR82_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500587.1"
                     /translation="MSEQPKQPKPSEEPEQPKARKEIPLVSVPNDRDMIAEEMEELFE
                     EDKVSHQHGSSEPEAD"
     gene            complement(1739..2098)
                     /locus_tag="VFR82_02970"
     CDS             complement(1739..2098)
                     /locus_tag="VFR82_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500588.1"
                     /translation="MSLPTVQEVEGRLASVQCAVCKGSSFGIDQRFMQADGEWRGICK
                     KCFYSFPVYTDMEFYLRTQPDVPYHLREISCTVCKHRGVSLDFRATMSVRESIYFVTC
                     GGCKRPFAEKSSLEAFE"
     gene            2212..2628
                     /locus_tag="VFR82_02975"
     CDS             2212..2628
                     /locus_tag="VFR82_02975"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HEU4500589.1"
                     /translation="MYQITDTLYVGNILEAERPPAHIEAVLLVAEEFTPNAPAGVIYE
                     RIPLKEFGEAKPASLNRAIDWIARHHPDKHVMVCCRAGMGRSVSVVMAYLCCVQKMEY
                     PDVFQLIMSRRPGACPLPNIQATIKQVHELRQARRS"
     gene            2685..3587
                     /gene="mutM"
                     /locus_tag="VFR82_02980"
     CDS             2685..3587
                     /gene="mutM"
                     /locus_tag="VFR82_02980"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEU4500590.1"
                     /translation="MPELPEAEVVARQIRARLVGAMLTGSWFGRPDIVREGYRNRNWY
                     AGSVLRAVERFGKSVVLRLEHQEETRYIVAELGMTGLLLFPAVQTRFPQHVHVRLTFT
                     GGAESELRYWNPRRFGRISLFDQSGLEHYLDRRFGCDPLTVTPEKFLTIIKERAGRLK
                     ALMMHQQAIAGIGNIYANEILFRARLHPNVRASRLPVAKVEELLRITREVLLEAISYG
                     GSSVRDFFAPDGSEGRFKGRHLVYGKEGLPCPNGCGRVIRRLQSERSSFICPACQAHR
                     PRRQRTSPPAAKNNSHLERSLTTA"
     gene            3707..4906
                     /locus_tag="VFR82_02985"
     CDS             3707..4906
                     /locus_tag="VFR82_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HEU4500591.1"
                     /translation="MAKVLEGPGMGLMKKWGIHVPHYAVVTSADELAKLGHANDWMKH
                     TKLVAKAHEALGSRFKLGLVKVGLDLSAAIAATKEMIGRQVGSINVSQVIVSEMVAHK
                     EEYYCAVKSTREGSEVLVANCGGIEVESNWDRVKRLCLEVGQQPTAEALEKLVKDDGF
                     TGPLVKKMADFAAKMFTCFDNEEAQYLEVNPVVIRESDGELVALDAVTLLDGDAKFRH
                     PDWNFQFAAEFGRAYSKDEMEVMAVDSKIKGSVKFIEIPGGDTAMLPAGGGASVYYSD
                     AVVARGGKLANYAEYSGDPPDWAVEVLTEKVCSLPGIRNIIVGGAIANFTDVKKTFGG
                     IINGFRKAKSGGKLKNVRIWVRRGGPREKEGLDAMRALKDEGFDINVFDRNTPLTDIV
                     DKALHKQ"
     gene            4992..6812
                     /locus_tag="VFR82_02990"
     CDS             4992..6812
                     /locus_tag="VFR82_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEU4500592.1"
                     /translation="MSILADKDTRVVIQGGQAGVNAARRMAEFCYLIKRPLNVEAFVY
                     PPDAGKTNEVPYGSGLIAIPVYKTIAEATKNHPTINTSLVYIGADRAMKGGMEALDDP
                     HIKVVSMITEGVPEKDAKLLGAHARKLGKVFNGPSSIGIISAGSCRLGVIGGAFDNLV
                     LSKLYREGSFGVVTKSGGLSNEIIWICSQFADGITTAIGIGGDAYPGTDYVSYLEMFE
                     NDPQTKAVVIVGEMGGDLEERAAEWYGAKKRRIKLIGVVSGFCQESLPKGMKFGHAGA
                     KEGMKGEGSARSKSDALKKAGALVPPTFGALGPAIKETYQELLKSGQVKEPVEPTVLP
                     KLPKSVEEAMKADEVMVAPLIRTTISDDRGDEPCYDGYPASELINKGYEIPHVIGLLW
                     DKRLISKQEAEIIKRIMMLSADHGPCVSGAYATILAACAGIGLSQSVAAGLIMIGPRF
                     GGAVTDAGRWFKYAVDNKMNVDEFLAYMKKNVGPVPGIGHRVKSLRNPDKRVKELVGY
                     VKSLNMKTPCLDFALQVEQVTAAKKDNLILNVDGTMAAVLVDLGFPVDSLNGFFILSR
                     TIGLIGHWVDQKRQDSRLIRLFDYLVNYAAPKRREVPPLK"
     gene            6897..>7870
                     /locus_tag="VFR82_02995"
     CDS             6897..>7870
                     /locus_tag="VFR82_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEU4500593.1"
                     /translation="MSMDLAKKLYAKMPDACAKARAKFGRGLTLAEKILVSHADNLET
                     QTWERGKAMLALRPDRVAMQDATAQMAILQFMQAGKKKVAVPSTIHCDHLIRAEMGSE
                     KDLLRAMDENKEVYNFLASAAKKYGIGFWKPGAGIIHQVVLENYAFPGCLIIGTDSHT
                     PNGGGLGGLAIGVGGADAGEVMAGLPWEVLHPKLIGVKLTGKLSGWASPKDVILYLCG
                     LLTVKGGTNKIVEYFGPGAETISATGKGTICNMGAELGATTSVFPFDQKMVAYMKITD
                     RSDLAELALANKTLLVADPEVYQSPEKHYDQIVEVDLSTLEPHVVGPHT"
BASE COUNT         1730 a         2144 c         2327 g         1669 t
ORIGIN      
        1 cgatcagcaa gtggggttta tcaaagacgc cctgaaattc tcctatctgc tgttgagaac
       61 cgacgggcgg acaatcgccg agcgcagtcc acacactttc cgcatggtca gtgaattacg
      121 caagctcaaa ggcgatacaa gggcctggct gtgtaatgaa cgtggtcgat gcgaggtggg
      181 gagactagat cgcgcagaaa ccgacatgaa ctcaaggtgg gacgaatgtc agcggggcac
      241 catcgtgcgg attgaacata tcacgcgcaa ggacggggct tcgctgggaa gaatttccga
      301 ccgctctgcg gtccagattg tgcgttccgt atgagctctg tccacggagg aatatgagct
      361 tcatcacccc tgattatttc atacacgccg caaacatcct cttactgatt gcgtacagcg
      421 ttcgcgacgt cctgtggttg cgcctgttcg cggtggcagc ttccctcatt gccattcctt
      481 tttatctgtt tcagcctacc gtgttgtggg cgccgcttgc ctggagcgtg gtctttgcag
      541 cgatcaatct ccttcaatcg tggctgcttt tcgtcgagcg tcgtccagtc aagctgacgg
      601 ctgaagagga ggaggttggg cgacttgcct tacaggactt gccacctcgg aaaatccttc
      661 aggttttgag tatcggatcg tggatcacgg ctgaagttgg cgagcacctc atagagcggg
      721 gcaagcttcc tgactccgtt tcgctcattg ttcgtggcaa agtgcggata accaaagatg
      781 ggcgcatgct ccgggatttg atccccggtg acatcgtggg ctccgctctc atcctcaacg
      841 ggatcccctc caatgtggat gcggtgacag ttgagccact gcgtgccatg cgttggcaca
      901 ttgggacatt gcagaagtat ctgagcgccc atcccgagac acgaaccgtc atgcagcggc
      961 atctcgcccg tgaccttgcg ggaaagatcg ggcgcctcgt cacggaaccc tcctaaaccc
     1021 catactgaca tgttgctgat cgatgactca tcgaatgatc gctgatttgt ctatcactta
     1081 gctgaagcat cgaattggtt agaggtagca tgacccagcc ataggtgttg taaagggcac
     1141 gtggtcatga agcgactcgg ctccgtttca catgttttta gagcagcgct gaccggttct
     1201 gcgttgcgaa tcgtggaggc gaggagtacc gcgtttgccg aaatggtccg aaacatgagg
     1261 acggcggcgc atgtgtgata ttcaggctga gcggagccgg acctcaagga atttgcgacg
     1321 ccgagcggac actatctcat ccacccaccc tggcccgcgc gagacgcggg ccttgaccca
     1381 taaggcgcgg tcgagccaga gcgttgagca gagtataacg gtcagcgttg tttgattaac
     1441 taagcgtagg gaagcgagtc tggatgatca cagcggctga acgagggtga cttaatcggc
     1501 ttcaggttct gagcttccgt gttgatggga gactttgtct tcttcaaaga gttcctccat
     1561 ctcctcggcg atcatgtcgc gatcgttcgg gacagagacg agtgggatct ccttgcgggc
     1621 tttcggctgc tcaggctcct cggatggctt gggttgtttt ggctgctcac tcatcagtac
     1681 agtatacacc aggctcatag caggatgttg aaaaagtcgc tcagcaccgg ttggactctt
     1741 attcgaaggc ttcgagggag gatttctcgg cgaagggccg cttacagccg ccacaggtga
     1801 caaagtatat cgactcgcgg accgacatcg ttgcgcgaaa atccaggctc acccctcggt
     1861 gcttgcagac cgtacaggag atttctcgta gatggtaggg cacatcaggc tgggtgcgga
     1921 gatagaactc catgtcggtg tagacaggga agctgtaaaa gcacttctta caaattcccc
     1981 gccattcgcc atcggcctgc atgaaccgct gatcgatgcc gaagctcgac cctttgcaga
     2041 cagcacattg gaccgatgcg agtcggcctt caacctcttg aaccgttggt agcgacattc
     2101 aattgcccct cttgtttgga gtatacgttc ggaaaaactc gactgcaacc ggtgtcgtca
     2161 agctttctgc ttgacctcca tgccggcctc tctaatactg ggactgactc catgtatcaa
     2221 attaccgata cgctttatgt gggaaatatt ctggaagcgg agcggcctcc ggctcacatt
     2281 gaggcggtgc ttctcgttgc ggaggaattt accccgaatg cgccagccgg cgtcatctat
     2341 gagagaattc cgttgaaaga attcggggaa gcgaagccag cttccctgaa ccgggccatt
     2401 gactggatcg cgcgccatca tccggataag catgtgatgg tttgctgcag ggccggaatg
     2461 ggccgttcag tgtccgttgt catggcctat ctctgttgcg tccagaaaat ggagtacccg
     2521 gatgtctttc aactgatcat gagtcgccgg ccgggagcct gtccgcttcc caacatccaa
     2581 gccaccatta agcaggttca tgaactccga caagcccggc gcagctagat cgcacgacgc
     2641 ctctgccctt gccttttctc ccagccaatg gcctaatcag agacatgcct gaacttcctg
     2701 aagccgaagt cgtcgcccgc cagattcgcg cccgcttggt tggggccatg ttgacgggca
     2761 gttggttcgg tcgacccgat atcgttcgcg aaggctatcg caatcgcaac tggtatgcgg
     2821 gctcagtgtt acgtgctgtc gagcggttcg gcaagagcgt ggtattgcgg ttggaacacc
     2881 aggaagaaac ccgatatatc gtggctgaac tggggatgac gggcctcctg ctttttccgg
     2941 ccgtgcagac gaggtttccc cagcacgtcc atgtgcgtct gacctttacg ggaggcgctg
     3001 agtcggagct ccgttattgg aatcctcgcc gattcggtcg gatctcgttg ttcgaccaat
     3061 cgggactcga acactatctc gatcggcggt tcgggtgcga tcccttgacc gtcacccccg
     3121 aaaagttcct cacgatcatc aaggaacgcg cagggcgctt gaaggccctc atgatgcatc
     3181 agcaggccat cgcaggaatc gggaacatct atgcgaacga gattctgttc cgagcacggt
     3241 tgcaccctaa cgttcgggcg agtcgtcttc ccgttgccaa ggtcgaggag ctgttacgga
     3301 tcacgcgcga ggtacttctt gaagcgattt cctatggcgg atcgagcgtg cgtgatttct
     3361 ttgctccgga cggcagcgag gggcgcttta aaggccgaca tttggtctat ggaaaggaag
     3421 ggctcccttg cccgaacggc tgcggccgag ttatcagacg tcttcaaagc gaacggagtt
     3481 catttatctg tcctgcttgt caggcacaca ggcctcgtcg acagcgtact tcacctccgg
     3541 ccgctaagaa taacagccac ttagagcgat ccctcaccac tgcctgaacc cctaggcctt
     3601 ttgatccctc atcgcttagt tcctctggtg tattgagtca tctacgcggt ttcgctacaa
     3661 tccgtctccc tttacatcga ttcttcatac cccaaggagc gacgccatgg ccaaggtgct
     3721 ggaaggtccc gggatgggac tgatgaagaa atggggcatt catgtccctc actacgcagt
     3781 cgtcacttca gccgacgaac tcgccaaact cggtcatgcc aacgactgga tgaaacacac
     3841 gaaactcgtg gccaaggcgc atgaagctct tggctcacgc tttaagcttg gcctcgtgaa
     3901 ggtcggactc gatctcagcg ccgcgattgc cgccacaaag gagatgattg ggcgccaagt
     3961 cggcagcatc aacgtctcac aagtcattgt ctcagagatg gtcgcgcaca aggaagaata
     4021 ctattgcgcc gtgaagtcca cccgcgaagg cagcgaagtt ctcgtggcca attgcggagg
     4081 tatcgaagtc gaatcgaact gggatcgagt gaagcgcctc tgtctcgagg tgggtcagca
     4141 acctacggcg gaagcactgg agaagctggt caaggatgac ggatttacag gcccgctcgt
     4201 caagaagatg gccgacttcg ccgccaagat gttcacctgt ttcgataacg aagaagccca
     4261 atacctggaa gtgaaccccg tcgtcatccg tgaaagcgac ggcgaattgg tggccctgga
     4321 tgccgtgacg cttctggatg gggacgccaa gttccgccat cccgattgga atttccaatt
     4381 tgcagctgaa ttcggccggg cctacagtaa ggatgagatg gaagtcatgg cggtcgatag
     4441 caagatcaag ggctcggtaa aattcatcga aattccgggt ggcgataccg caatgcttcc
     4501 ggccggaggc ggtgccagcg tctattactc ggacgcagtg gtggcgcgcg gaggtaaatt
     4561 agctaattat gcggagtatt ccggcgatcc ccccgactgg gccgtcgaag tgctgaccga
     4621 gaaagtctgc tcgctgccgg gcatcaggaa catcatcgtg ggtggcgcca tcgccaattt
     4681 caccgacgtg aagaagacct tcggcggcat tatcaacggc ttccgtaagg cgaagtcggg
     4741 cggcaaattg aagaacgtac gtatttgggt gcgccgcggc ggccctcgcg agaaggaagg
     4801 cctcgatgcc atgcgggcgc tgaaggatga aggattcgat ataaacgtct tcgaccgcaa
     4861 caccccgctg acggacatcg tcgataaggc gcttcacaaa cagtaacgaa ggctgggacg
     4921 gcctgaggcc aggagcattc gcttggctcc cgcctcggta ttcagtttcg atggaataaa
     4981 gggagattcc gatgagcatt ctggctgaca aagacacccg cgtcgtcatt cagggcgggc
     5041 aggccggtgt gaacgccgca cgacgtatgg cggaattctg ctacttgatc aagcggccgc
     5101 tcaatgttga agcgttcgtc tatccgccgg atgccggcaa aaccaacgag gttccctacg
     5161 gcagcggact cattgccatc cccgtataca agaccatcgc cgaagcgacc aagaatcatc
     5221 ccacgatcaa cacgagcctg gtttacatcg gtgcggaccg tgcgatgaag ggcggcatgg
     5281 aagcgcttga cgacccgcac atcaaggtcg tgtccatgat caccgaaggc gtcccggaga
     5341 aggacgccaa gctgctgggg gcacatgcac ggaaactcgg gaaggtattt aacggaccgt
     5401 cttcgatcgg gatcatctcg gccggttcct gtcggctggg cgtgatcggc ggtgcgtttg
     5461 acaacctggt tctctcgaag ctctatcgcg aagggtcctt cggcgtcgtc acaaaatcag
     5521 gcggcctctc caacgagatc atctggatct gttcacagtt tgccgacggc atcacgaccg
     5581 cgatcggcat cggcggcgac gcgtatcctg gcaccgatta cgtgagctat ctggagatgt
     5641 tcgaaaacga tccgcagacg aaagccgtcg tgatcgtcgg cgaaatgggg ggcgatctcg
     5701 aggagagggc ggcggagtgg tacggcgcga aaaagcgccg catcaagctg atcggtgtcg
     5761 tgtccggttt ctgccaggaa agtttaccga agggcatgaa gttcggccat gcaggcgcca
     5821 aggaagggat gaaaggcgag ggctcggcac gctccaagtc ggatgcactc aagaaggcgg
     5881 gggccctcgt gccgccgact ttcggagcgc tcggtcctgc gattaaggag acatatcagg
     5941 aactgctgaa gtcaggtcag gtcaaggaac cggtggagcc gacggtgctg ccgaaactgc
     6001 cgaagtcggt cgaggaggcc atgaaggcgg acgaagtgat ggttgcgccg ctcatccgga
     6061 ccaccatcag cgacgatcgt ggcgacgaac cctgctacga cggctatccg gcttccgaac
     6121 tgatcaacaa aggctacgag attccccacg tgatcggctt gctctgggac aagcggctca
     6181 tctcgaaaca ggaagcggaa atcatcaagc gcatcatgat gctgtcggcc gatcacggcc
     6241 cctgcgtgag cggggcctat gcgacgatcc tggcggcttg cgccggaatc ggcctgtccc
     6301 aatccgtggc cgcaggtctc atcatgatcg gcccgcgttt cggtggggcg gtcaccgacg
     6361 ccggtcgctg gttcaagtat gccgtcgaca acaaaatgaa cgtggacgaa tttctggcct
     6421 atatgaagaa gaacgtcggt ccggttccgg ggatcggcca tcgagtgaaa agcctgcgta
     6481 atccggacaa gcgcgtcaaa gagctcgttg gctacgtcaa gagtctcaac atgaagacgc
     6541 cgtgcctcga ttttgcgctg caggtggagc aggtcacggc cgcgaagaag gacaatctca
     6601 ttttgaatgt ggacggcacc atggcggccg tcctggtcga tctaggcttc cccgtggaca
     6661 gtctgaacgg gttctttatt ctgtcgagga cgatcggatt aatcggccat tgggtcgatc
     6721 agaagcgcca ggacagccgg ttgattcggc tgtttgatta tctcgtcaac tatgcggcgc
     6781 ccaaacgaag agaagtgccg ccactgaaat aagtggtgag gcgctcgcgt gcgagcgagt
     6841 gaatgagttc aacgttcgag atttgaaaat ccacattcca cattgaggat gtcatcatgt
     6901 cgatggacct tgcgaaaaaa ctctatgcca aaatgcccga cgcctgcgcc aaggccaggg
     6961 cgaagttcgg ccgcgggctg acgttggccg agaagattct cgtttctcac gccgacaact
     7021 tggagaccca aacctgggag cgcggcaagg ccatgctggc tctgcgcccg gatcgcgtgg
     7081 ccatgcagga cgcgaccgcg caaatggcca tcctgcagtt catgcaggca ggcaagaaga
     7141 aagtcgctgt gcctagcacc atccactgcg atcacctgat ccgggcagaa atgggttcgg
     7201 aaaaagatct cctgcgtgcg atggacgaga ataaggaggt ctacaacttc ctggcctctg
     7261 cggcgaaaaa gtacgggatc gggttctgga agcccggcgc ggggatcatc caccaagtcg
     7321 tcctggaaaa ttatgcgttt cccggctgct taatcattgg gaccgactcc catacaccca
     7381 acggcggagg cctaggaggg ttggccattg gcgtcggcgg agccgatgcc ggtgaagtga
     7441 tggctggtct gccctgggaa gtcctccatc ccaaactcat cggtgtcaaa ttgacgggca
     7501 aattgagcgg ctgggcatcg ccgaaagacg tgattcttta tctctgcggt ctgctgacgg
     7561 tgaagggcgg cacaaataag atcgtcgaat acttcggtcc gggggcagaa accatcagcg
     7621 cgacaggtaa gggcacgatt tgcaacatgg gagccgagct gggagcgacc acctccgtgt
     7681 tcccgttcga tcaaaagatg gtcgcctaca tgaaaatcac agatcgatcc gatttggccg
     7741 agctggctct ggccaacaag accctccttg ttgccgatcc cgaggtctat caatcaccag
     7801 aaaagcatta cgaccagatc gtggaagtgg acctctccac gcttgagccg catgtcgtcg
     7861 gtccacatac
//
LOCUS       DASTQY010000093        10085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1791912, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000093 DASTQY010000000
VERSION     DASTQY010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10085
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791912"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VFR82_03000"
     CDS             complement(<1..382)
                     /locus_tag="VFR82_03000"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247026.1"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU4500594.1"
                     /translation="MKTLTITPGRPLRGTIVVPGDKSITHRAIILAALAEGMGRIKRY
                     CRGEDCLNTMRALQALGVRIDEADDELRVHGKGLWGLSEPAGVLDCGNSGTGIRLLTG
                     LLAGQDFFSVLTGDGSIRRRPMGRI"
     gene            complement(408..1289)
                     /locus_tag="VFR82_03005"
     CDS             complement(408..1289)
                     /locus_tag="VFR82_03005"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEU4500595.1"
                     /translation="MASHFRQAAIIGVGLIGGSLGMILRRKGFASSVVGVGRRIENLK
                     LAVKLGAIDQYVVDAKEGVKDADIVVLATPVDTYQRHLEEWASCLKSGAIVSDVGSVK
                     GELVEQCERLMPDGVHFVGAHPIAGKEKTGVAAGSEDLFSGMRCIVTPTKQTNPQALE
                     RVRTLWQEAGSTVLTMDPHLHDKILGAVSHLPHVVAFALMGALIDIRTEVPSLDLAGH
                     SGGGLRDTTRIAASSPEMWRDIFLWNRENVVAFIEAYERSLGDLKQLIRAGDVAGIEK
                     ALERAKQEREKFPVRTG"
     gene            complement(1289..2302)
                     /gene="aroF"
                     /locus_tag="VFR82_03010"
     CDS             complement(1289..2302)
                     /gene="aroF"
                     /locus_tag="VFR82_03010"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU4500596.1"
                     /translation="MIIVLKPEASEREVDHIVDRLRELGLKSQLSTGQERTIIGVIGD
                     DRILQNQPLTALPGVESVLPILAPWKLVSREFKKEGTIIDVGGVKIGGNKLAIMAGPC
                     AVERLELTVGIAHEVKAAGASVLRGGAYKPRTSPYSFQGLGREGLDYLAEARKQTGLP
                     VVSEILDTRDIELFLEKADIIQIGARNMQNFELLKEVGAYDKPVLLKRGLSATIKEFL
                     LSAEYVMSRGNRNVMLCERGIRTFETQYRNTLDLAAIPTLKELSHLPVIVDPSHATGK
                     WNLVAPMSKAAVAAGADGILIEVHSNPECALCDGEESIKPIKFKELMHDMRKIAVAVG
                     REV"
     gene            complement(2310..3401)
                     /gene="hisC"
                     /locus_tag="VFR82_03015"
     CDS             complement(2310..3401)
                     /gene="hisC"
                     /locus_tag="VFR82_03015"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEU4500597.1"
                     /translation="MTLHVHPDIAALSPYVPGKPIEELQRELGLSRVIKLASNENPLG
                     PSPKALAVINETAATLHRYPDGGAFKLRQALAHRWKVTDEQVILGNGSDEILGLVART
                     FLSPGDEAVMADHTFVIYNMEVMAAHGKAVTVPLKDWRHDVAAMASAITDRTRLVFLC
                     NPNNPTGTMVSAEEVAWLLARIPDHVVVVFDEAYFEYVRSSEFPDSMAWIKEGRNVIV
                     LRTFSKIYGLAGLRIGYGVTTGEIANLLNRVRPPFNANSIAQRAALAALDDDEHVARS
                     RAVNQIGMEQIANGLQALGLAPISSEANFVYVDVGRDGRGVFDAMLRHGVIVRHIEGR
                     MIRVTIGQAEENRVFLAALAQVLQQPELG"
     gene            complement(3406..4482)
                     /gene="pheA"
                     /locus_tag="VFR82_03020"
     CDS             complement(3406..4482)
                     /gene="pheA"
                     /locus_tag="VFR82_03020"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU4500598.1"
                     /translation="MPGDLSDYRKEIDRIDDEILRLLNERSKSVVQIGRLKMLQDAGA
                     NLHTPAREAAIIERLVAQNTGPFPSDAIRPVYREIMSASLSLEGPQKVAYLGPRATFT
                     HMACTQKFGASAQYVPVNSIKEVFSEVERGRANFGVVPIENTTEGVVNHTLDMFIDSN
                     LLIYGEVLQEVSHHLMSKSGAAEDIKKICSHPHAIAQCRNWLETNMPHVPVSEVASTA
                     RAAEMCLDDSSIAAIASELASQLYGLKVIKSRIEDNLNNFTRFLVLSQKPPQRTGKDK
                     TSLMLSIKDKVGALYDLLRPFASHGLSMTKIESRPSRRKAWEYIFFVDVEGHIEEERV
                     KKAVEEITGRCLFMKVLGSYPAHS"
     gene            complement(4656..5657)
                     /gene="ruvB"
                     /locus_tag="VFR82_03025"
     CDS             complement(4656..5657)
                     /gene="ruvB"
                     /locus_tag="VFR82_03025"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU4500599.1"
                     /translation="MTRVVNAQLTDEERSLEQTLRPQTLSEYIGQARMKDSLRICIEA
                     AKQRREPLDHAIFYGPPGLGKTTIAHIIAREMGGTFCATSGLVLTHAGDLAAILTNLQ
                     EHDVLFIDEIHRLPASVEEALYPAMEDFQLDLVVGQGPAARTVKLDLPRFTLVGATTK
                     AGSLTSPLRDRFGLVHRLEFYSPAELEVIVMRSARLLGIPIDQEGAAEIACRARGTPR
                     IVNRLIKRVRDYAQVKAQGAITRAVAQDALAWLGVDAAGFDEMDRHILLTIIDKFGGG
                     PVGVESLAAAVQEDKSTLEDVHEPYLIQAGFLDRTGRGRQVTKAALEHFKRPSSLLY"
     gene            complement(5676..6176)
                     /locus_tag="VFR82_03030"
     CDS             complement(5676..6176)
                     /locus_tag="VFR82_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500600.1"
                     /translation="MTAYRTSVLTASAITLVLIGHLPPALPSVAWADEASAEAKSIRK
                     TCGKCPDGYATTGVTEAPELCKGGDPTLAQCVPLGVNMLPVCGSCPDGYREVGHSSVP
                     ARCGSQDGGRLSQCQLENMGQQLPDPTQGGKICPPDCGSTPLPGRGTLPPPPKYLPAP
                     DQPHKD"
     gene            complement(6185..6793)
                     /gene="ruvA"
                     /locus_tag="VFR82_03035"
     CDS             complement(6185..6793)
                     /gene="ruvA"
                     /locus_tag="VFR82_03035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU4500601.1"
                     /translation="MIAHLTGRLAFKAPTHLALDVHGVGYEVLIPLSTYYNLPNADES
                     LTLSIHTHVREDAIQLFGFSSRQEKDAFVLLMSVSGIGPKSALGILSALPVADLASAI
                     QSGDVEKLETVPGIGKKSAGRLVLELKDKLIKIHPGLTATVDQSVAGSDDVFDDALSA
                     LTNLGYRATDAKDALKTVRKSPAQPLSLQELIRETLKHLAKG"
     gene            complement(6813..7571)
                     /locus_tag="VFR82_03040"
     CDS             complement(6813..7571)
                     /locus_tag="VFR82_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU4500602.1"
                     /translation="MGGHSHWATIKRHKGAQDAKRGKIFTRIIREVTIAARTGGDPDG
                     NPRLRLAIAKAKEANMPGDTLKKAIQRGTGELPGVTYEEFSLEGYGPGGTALLIEITS
                     DNRNRTVAEIRSVLTKNHGNMAEAGAVSWQFQKKGLLTIDKSKSDEDTLLSLALEAGA
                     EDVKVGEKTFEVITNPHDFDAVKKSLLDAKIESSMAEITYIPQNTIRLEEKGAEQMLK
                     LMEILDEHDDVQKVHANFDIPDDVMEKVAAATAG"
     gene            complement(7694..8458)
                     /locus_tag="VFR82_03045"
     CDS             complement(7694..8458)
                     /locus_tag="VFR82_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500603.1"
                     /translation="MKVDCILHIVRRCVLTSGFLILVFLAPAAAQQSSSFAVDSPHTA
                     YHGQHGRPTPEPWEGSAKGIAYSERNHHIAGLLVILMGVAELTHAMRLAALSWVRLLL
                     PSALLCAGAFLLIWSDHEAWPIGSLTFGQSFFGDDHEIIQHKFYGILALVVGSVELFR
                     RLGRAGHRVWASPLPLMAIVGGLMLFGHSHGFHPSAQKIATHHAIMGTMALTAGSSKL
                     FSTWLNPTPGGAAVRWDWIWASLILLIGTQLLIYSE"
     gene            complement(8546..9295)
                     /locus_tag="VFR82_03050"
     CDS             complement(8546..9295)
                     /locus_tag="VFR82_03050"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HEU4500604.1"
                     /translation="MKFVCLTCETYMNFEKVEKPGEGSLGVFFGCPSCGAKFSMVTNP
                     GETQMVSSLGVQLGGRTAQAQPFEMTRGTLKEEAQAGSGQMAAYLNERIQGGKAVTAS
                     DHSPKSGEKSSGGCPFSAMVAEMGLTSGGKPANGGPAKSDFTWTPDAKEKLDRLPAFV
                     KPMVQGSVEAFARKHGYETITLQVMDDSKNDSPNGMSWTPEAQQRLDNIPDFIRPMAR
                     KEIERLAKERGIATITAEVMDDAKDKFMKFM"
     gene            complement(9312..>10085)
                     /locus_tag="VFR82_03055"
     CDS             complement(9312..>10085)
                     /locus_tag="VFR82_03055"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEU4500605.1"
                     /translation="KGGVGKSMTTVNLALALARQHARVGLLDVDLNGPCVPRMLGLHG
                     RSLTITPEGAVPPVGPLGVKVASMDFFLGTSSPVRWKGPMDVSPVWLGLMEMNVIREF
                     LADVAWGELDYLLADLPPGAAADKPPVIAGFIPDLAGAIVVTTPSEVASDVVQKSVTY
                     ARDMGIKVLGIVENMSEYRCPSCGELNELFEGNTEAMCEALDLPLLGRIPFDRKLART
                     FDKGEPLLDETYPTIQRYQDIAGRIRTLLDYKRVLAEKL"
BASE COUNT         2160 a         3015 c         2738 g         2172 t
ORIGIN      
        1 cgattcgtcc catgggacga cgccgaatgg acccatctcc cgtgaggaca ctaaaaaaat
       61 cctgaccggc caggagccca gtaagcagac ggattccggt acccgagttt ccgcaatcga
      121 ggacccctgc cggttcgctc agcccccaca gaccctttcc atgcacacgg agctcatcgt
      181 cggcttcatc gatccgcact cccaacgcct gcaacgcacg catggtattg aggcagtctt
      241 caccgcggca atagcgttta atgcgtccca tgccttcggc gagagccgca agaataatcg
      301 cccgatgcgt aatggatttg tcgccaggaa caacaattgt tccccgtagc ggcctgccgg
      361 gtgtaatcgt caacgtcttc aagtttgcct gtgctcctcc agccgaccta tccggtgcgg
      421 accgggaatt tctcacgttc ctgcttcgct cgttctaacg ccttttcgat tcccgccaca
      481 tcgcccgcgc gaatcaactg tttcaagtcg cccaacgagc gttcatacgc ttcgataaag
      541 gcgaccacat tttcgcggtt ccagagaaaa atgtcgcgcc acatctcagg agagctggcc
      601 gcgatgcggg tcgtatcccg caacccgcct ccggaatggc cggccagatc gagcgacgga
      661 acctccgtac ggatatcgat caaggcgccc attaatgcaa aggcgacgac gtgcggcaga
      721 tgactcaccg ctccaagaat tttgtcgtgg agatgcggat ccatcgtgag caccgtcgac
      781 cccgcttcct gccacagcgt ccttacccgt tcgagtgcct gcggattcgt ctgtttggtg
      841 ggcgtcacaa tgcaccgcat tcccgaaaaa aggtcctccg accccgcggc gacgcccgtt
      901 ttctccttgc cggcgatggg atgcgcgccg acaaaatgga cgccgtccgg catgagccgt
      961 tcgcactgct cgactaactc gcctttcaca ctccccacgt ccgacacaat cgcaccagac
     1021 ttcaaacacg aggcccattc ctccaagtgg cgttgatagg tatcaaccgg cgtcgccaag
     1081 acaacgatat cggcatcttt cacgccctcc ttggcatcta cgacgtactg gtcgatcgcc
     1141 cctaatttga ccgccagctt gaggttctca atgcgccgtc ccacgcccac gacgctggac
     1201 gcgaatcctt tccggcgaag gatcatgccg agggaaccac cgatcaagcc gacaccgata
     1261 atggcggcct ggcgaaaatg cgacgccatt acacttctct tccgaccgcg acagcgatct
     1321 tgcgcatatc atgcatcaat tccttgaact tgatcggctt gatcgactct tcgccatcgc
     1381 ataatgcgca ttcgggattc gaatggactt caatgagaat gccgtcggcg ccggcggcca
     1441 cggcagcctt ggacattgga gccaccagat tccatttgcc ggtagcatga ctgggatcga
     1501 caatgaccgg cagatgtgac agctccttca aggtgggaat agcggcgaga tcgagggtgt
     1561 tgcggtactg tgtctcgaag gtgcgtatgc cccgctcaca gagcatcacg ttccgattcc
     1621 cacgggacat gacgtactcc gctgacaaca gaaattcctt gattgtagcc gacagcccac
     1681 gcttcaagag tacaggcttg tcgtaggcgc ccacttcctt tagcagttcg aagttttgca
     1741 tgtttcgcgc accgatctgg atgatgtcag ccttttcgag aaacaactcg atgtcgcggg
     1801 tgtcgagaat ttcgcttacc actggcaagc ctgtctgttt tctggcctcc gccagatagt
     1861 ccaacccttc acgacccagt ccttgaaacg agtagggaga ggtgcgtggc ttgtacgcgc
     1921 cgccccgtag cacgctggct cccgccgcct tgacctcatg cgcaatgccg acggtgagtt
     1981 cgaggcgttc tacggcacac gggcccgcca tgattgcgag cttgttgcct ccgattttga
     2041 cccctccgac atcaatgatc gtgccttctt tcttgaattc tcggctgacc agtttccagg
     2101 gagccagaat cggcagcacg ctttcaacgc caggtaaggc tgtgagcggc tggttctgaa
     2161 gtatgcgatc atcgccaatc accccgatga tcgttcgttc ctgtccggtc gagagctgcg
     2221 atttcaaccc cagttcacgg agccgatcga cgatatgatc gacttcgcgt tccgatgcct
     2281 ccggcttcaa cacgatgatc ataacaacct caccctagtt caggctgctg taagacctgt
     2341 gcaagtgcag ccaagaaaac ccggttttct tctgcctgtc cgatcgtcac ccgtatcata
     2401 cgcccttcga tatgacggac gatcacgccg tgacgcaaca tcgcatcgaa tacgccacgg
     2461 ccgtcgcgac cgacatcaac atagacgaaa ttggcttcgc tactgatcgg cgccaatccg
     2521 agcgcctgca atccgttcgc gatctgttcc atgccgattt gattcactgc ccggctccga
     2581 gcgacatgct cgtcatcatc cagcgcggca agcgctgcgc gctgagcaat gctattggca
     2641 ttgaagggtg gcctcacacg gttcaaaaga ttcgcaatct cgcctgtagt gactccataa
     2701 ccgatgcgca atccggcgag gccgtaaatt ttagagaacg tccgcaacac gatgacattg
     2761 cgtccttcct tgatccaggc catggaatcg gggaactcgg aactgcgcac atactcaaaa
     2821 taggcctcat cgaagaccac cacgacgtgg tcaggaatgc gcgcgagcag ccatgcaacc
     2881 tcctcggccg acaccatagt tcccgtggga ttgttgggat tgcacaggaa cacgaggcgc
     2941 gtccgatccg tgatcgcact cgccatcgca gcgacatcat ggcgccagtc tttcaacgga
     3001 acggtgaccg ccttgccatg cgcggccatg acttccatgt tgtaaatgac gaaggtgtgg
     3061 tcggccatga ccgcttcgtc accgggcgac agaaacgttc gagcgacaag cccgagaatt
     3121 tcgtctgagc cgttgccaag aatgacctgc tcgtctgtca ctttccatcg atgggccagc
     3181 gcctggcgca atttgaacgc ccctccgtcc ggataccgat gcaatgtcgc cgcagtctcg
     3241 ttgatcaccg ccaacgcttt cggcgacggt cccaacggat tttcgtttga ggccagctta
     3301 atgacacggc taagacccaa ttcacgttgg agttcttcga tgggcttacc cggcacgtaa
     3361 ggactcaatg cggcaatatc aggatggacg tgaagcgtca tggaatcagc tatgggctgg
     3421 ataggagccg agcactttca tgaagagaca gcgtccggtg atttcctcga ccgccttctt
     3481 gacccgctct tcttcaatat ggccctcgac gtcgacgaaa aaaatgtatt cccaggcctt
     3541 gcgccgtgag gggcgcgact cgatcttggt catactcaat ccatgagacg caaagggacg
     3601 caacaagtca tagagcgcac ctaccttgtc tttaatggac agcatgagcg atgtcttgtc
     3661 tttgcctgtg cgttgtggtg gtttttgcga caacaccagg aaccgcgtaa agttgttgag
     3721 attgtcttcg atgcgggact tgatgacctt taatccatag agctgcgaag ccaattcgga
     3781 ggcaatggcc gcgatcgagg aatcgtccag gcacatctcg gctgcccggg ccgtactcgc
     3841 gacctccgaa accggaacgt ggggcatatt cgtctcgagc cagtttcggc attgggcgat
     3901 ggcgtgggga tgggaacaga ttttctttat gtcctccgca gccccggact tcgacatcag
     3961 atgatgggag acttcctgaa gaacctcgcc atagatcagg aggttggaat cgataaacat
     4021 gtcgagggta tggttcacca cgccctctgt cgtattctcg atcggcacga cgccgaaatt
     4081 cgcacgaccc cgttcgactt cgctgaacac ttccttgatg ctgttaaccg gcacatactg
     4141 cgcggacgcg ccaaactttt gtgtgcaggc catatgggtg aacgtcgctc gcggtccaag
     4201 gtaggccacc ttctgtggac cttccaggga cagggacgcc gacatgattt cccggtacac
     4261 ggggcgaatg gcatcggaag ggaaaggacc ggtattttgg gcgacaagcc gctcgataat
     4321 ggcggcctcg cgtgcaggcg tatgcaggtt cgccccggca tcttgcagca tcttcaatct
     4381 gccgatttga accacacttt tagaacgctc gttgaggaga cgtaagatct cgtcatcgat
     4441 gcgatcgatt tctttccggt aatcggaaag gtcaccgggc atgaatggga ccccctccac
     4501 ccgtgcccca taagttggac gagcgtccag aggcaggacg ggcgtgagta agcaacgaat
     4561 gatacaggaa ccgccctcat gaaagcaagg agagcctaag actttcatga acttgtgtga
     4621 tcctgaccgc gggcggctcc gaaacaaaag aggggtcagt agagaagaga agacggccgc
     4681 ttgaagtgct cgagcgcggc cttcgtgacc tgccggcctc gaccggtccg gtccagaaag
     4741 ccagcttgga tgagataggg ctcgtgaacg tcctcgaggg tgctcttgtc ttcttgaacg
     4801 gcggcggcga gagactccac ccccaccggt ccgccaccga atttatcgat aatcgtcagg
     4861 aggatatgcc gatccatctc atcgaaaccg gctgcatcga cgcccagcca tgccaaggca
     4921 tcttgggcga ccgctcgggt aatcgcgccc tgcgccttca cttgcgcata gtcacggacg
     4981 cgcttgatca gccggttaac gatgcgaggt gtgccgcgtg cgcggcatgc gatctccgca
     5041 gcaccttcct gatcaatggg gatgccgagc aaacgagccg agcgcatgac gatgacttcc
     5101 agttcggccg gtgaatagaa ttccagccga tgaacgagcc cgaagcggtc tcggagcggc
     5161 gaagtcagag accctgcctt cgtcgtcgct cccacgagcg tgaaccgagg gaggtccagc
     5221 ttgaccgtcc gcgccgctgg cccttgcccg acgaccagat cgagctggaa atcctccatt
     5281 gcgggataca gggcttcttc gaccgaagcg ggcaaacgat gaatctcatc aatgaagagg
     5341 acgtcgtgtt cctgtaggtt tgtcagaata gctgccagat caccggcatg cgttaagacg
     5401 agcccggagg tggcgcaaaa ggttccaccc atttcacgcg caatgatatg agcgatggta
     5461 gtctttccca gccccggcgg accatagaaa atcgcatgat ccagaggctc gcgtcgctgt
     5521 tttgccgcct cgatgcagat tcgcaaggaa tctttcatcc gcgcctgccc gatgtactcg
     5581 ctaagcgttt gcggacggag ggtctgctcc aagctccgtt cttcatccgt cagctgggca
     5641 ttgacgacac gagtcatgat tcgtcgatct ccacgtcaat ccttatgcgg ttgatccggc
     5701 gccgggaggt atttcggcgg aggtggtagc gtgcctcgtc ctggaagcgg cgtactgccg
     5761 caatcagggg gacagatttt tccgccctgc gtcggatcag ggagctgttg ccccatgttc
     5821 tccaattggc actgagaaag ccgtcctccg tcctggcttc cacaacgtgc cggcacggat
     5881 gaatggccga cttctcgata cccgtcagga caggatccgc agaccggcag catgttcaca
     5941 cccaggggaa cgcactgcgc caatgtggga tcgccgcctt tgcatagttc aggcgcctcg
     6001 gtcacgccgg ttgtggcata tccatctgga cacttcccac aggtcttacg gatgcttttc
     6061 gcctccgcag aggcttcgtc cgcccacgcg acggaaggca gtgcaggcgg aaggtggccg
     6121 atcagtacca gcgtaatcgc tgaagcggtc aagacgctcg tcctgtatgc agtcatgagc
     6181 agggtcatcc cttggccaga tgtttcaagg tctcgcgaat gagctcctgc agactcaacg
     6241 gctgagcggg tgacttccga accgttttga gtgcgtcttt tgcgtcggtt gcccgatacc
     6301 cgagattggt gagagccgaa agggcgtcgt caaagacgtc atcgctcccg gcgacgctct
     6361 gatcaacggt cgccgtcaat ccggggtgga tttttatcaa cttatctttc agttcgagta
     6421 ccagtcgacc ggctgatttc ttcccgatcc cgggaaccgt ttctaatttc tcgacatcgc
     6481 ccgactggat tgcagaggcc agatcggcaa cgggcaaggc ggacaggatg cccagcgctg
     6541 actttggccc gatcccggag acactcatga gcagtacaaa ggcgtctttc tcctggcggc
     6601 tactaaatcc gaacagttgg atggcatctt ccctgacgtg ggtatggatg ctgagggtta
     6661 aactctcgtc cgcattcggg aggttatagt aggtgctgag agggatgagc acctcgtatc
     6721 caaccccatg gacatcgagg gccagatgcg tgggcgcctt gaaggccagt cggcctgtca
     6781 ggtgggcgat catgcgtggt ggctgatgac tactagccgg ctgtcgcggc cgcgactttt
     6841 tccatcacgt cgtcgggaat atcgaaattc gcgtgcacct tttggacatc gtcatgctcg
     6901 tccaggatct ccatgagttt caacatttgc tcggcgccct tttcttccag cctgatcgta
     6961 ttttggggta tataggtaat ttcggccatg gatgactcaa tcttcgcatc gagcagggat
     7021 ttcttcaccg catcgaagtc gtgaggatta gtaatgactt caaatgtttt ctccccgact
     7081 ttcacatcct ctgctcccgc ctcgagcgca agcgaaagaa gggtgtcttc gtcactcttg
     7141 gacttgtcga tcgtcagcaa tccctttttc tgaaactgcc aggagacggc tcccgcttcg
     7201 gccatgttgc cgtgattttt cgtgaggaca ctgcggattt ccgccacggt ccgattccgg
     7261 ttgtcgctgg taatctcgat caacagcgca gttccacccg gaccatagcc ttccaaagaa
     7321 aattcctcat aggtcacacc aggcaactcg cctgtcccgc gctgaatcgc tttcttcaag
     7381 gtgtcaccgg gcatgttggc ttccttggcc ttggcaatag ccagccgcaa cctgggattg
     7441 ccgtccggat ctccccccgt acgcgccgcg atcgtgactt cgcggatgat cctcgtaaag
     7501 atcttccccc gtttggcgtc ctgggctcct ttatgtcgtt tgatcgtcgc ccagtgacta
     7561 tgaccaccca tgactgctcc attcggccgc cgtaaggcgt accagcgctc caattatatg
     7621 cagtgaagtt gtagacctaa cacagggttt ggaggggtgt caattacggt gcgggcgaca
     7681 aaatgaacga cacttattcg gaatagatga ggagctgcgt tccaatcagc aggatcaaag
     7741 acgcccaaat ccaatcccat ctaaccgcgg ctcctccggg cgtcggattg agccacgtgg
     7801 agaacagctt tgacgaccct gcggtgaggg ccattgttcc catgatcgca tggtgcgtcg
     7861 caatcttctg tgcagaagga tggaatccgt gtgagtggcc gaacagcatc aagccgccga
     7921 ctatggccat gagaggtagc ggggaggccc acactctatg gccggcacgg cccaatcgtc
     7981 taaacaactc gacgctcccg accaccaatg caagaatccc atagaacttg tgctgaataa
     8041 tctcgtggtc gtcgccgaaa aacgattggc cgaaggtcaa cgagccgatg ggccaggctt
     8101 catgatcgct ccatatcaga aggaacgccc cagcacacaa gagagcggag ggcaaaagaa
     8161 gccggaccca gctcaaggcg gcaagccgca tggcatgggt gagctcagcg acgcccatca
     8221 agataaccag aaggccggct atgtgatgat tgcgctcaga ataggcgatg cctttcgccg
     8281 aaccttccca aggctctggc gtagggcgac cgtgctgccc gtgatacgca gtgtgcggcg
     8341 aatcgacagc aaacgatgat gactgttgag cagcggcagg cgccaagaag accaggatca
     8401 aaaacccact cgtcagcacg cagcgtcgca cgatgtggag aatgcagtcg accttcatgt
     8461 aggcatgtta gaggcttctc acgtgaaagg cccatccggt tccccggatg ggcctcatgt
     8521 ttcaagaccg ggggcgatgc ctatgttaca tgaacttcat gaacttgtcc ttggcatcgt
     8581 ccatgacttc tgcggtgatc gtcgcaatac cgcgttcttt ggccagccgt tcaatttcct
     8641 ttcgcgccat aggacggata aagtcaggaa tattgtcgag ccgctgctgc gcttccggcg
     8701 tccacgacat gccgtttggc gaatcattct ttgagtcgtc catcacttga agagtaatcg
     8761 tctcatagcc gtgcttgcgg gcgaaggcct caacactgcc ctgcaccatc ggcttgacga
     8821 aagcgggaag cctatccagt ttctctttgg catccggcgt ccaggtaaaa tcggacttgg
     8881 ccggacctcc attggccggt ttgccgcctg aggtcaagcc catttccgcc accatagccg
     8941 agaaggggca ccccccgctg cttttctcgc cggattttgg cgaatggtca gacgccgtca
     9001 cagccttacc gccttggatt ctttcgttta aataggccgc catctgaccg gaaccggctt
     9061 gcgcttcttc cttcaacgtc cctcgggtca tttcaaatgg ctgagcctgt gcagtccgtc
     9121 cacccaactg gaccccaagc gagctgacca tctgcgtttc accgggattg gtcaccatcg
     9181 agaacttcgc accgcacgac ggacagccga agaagacgcc gagcgatcct tcgccaggct
     9241 tctcgacctt ctcgaagttc atatatgttt cgcaggtgag acacacaaat ttcatgacag
     9301 ctcctctcag ctcacaattt ttccgccaac actctcttat aatccaatag agtcctaatc
     9361 cgcccggcga tgtcctgata acgctggatg gtcgggtagg tctcatcaag caatggctca
     9421 cccttgtcaa acgtgcgggc gagcttgcga tcaaaaggaa tgcggcccaa taaagggaga
     9481 tcaagcgcct cgcacatggc ttcggtgttg ccttcgaaca actcgttgag ttcgccacat
     9541 gaggggcacc ggtactcgct catattttca acgataccca acaccttgat gcccatgtcc
     9601 cgggcgtacg tgacggattt ctgcacgacg tcggatgcca cttccgaagg cgtcgtcaca
     9661 acaatcgcac ctgccaaatc ggggatgaaa ccagcaatga ccggcggctt gtcggccgcg
     9721 gcgccaggag gcaagtcggc cagcaagtaa tccagttctc cccatgccac gtcggcgaga
     9781 aactcgcgaa tcacgttcat ctccatcaag cccaaccaga ccgggctgac atccatagga
     9841 cccttccagc ggaccggcga tgacgtcccc agaaagaaat ccatggaagc cacttttacc
     9901 ccgagtggcc cgaccggagg aacggccccc tcaggcgtta tcgtcaacga acgcccatga
     9961 aggcccaaca tccggggaac acaggggccg ttgaggtcga cgtctaaaag cccgactcgc
    10021 gcgtgctgac gggccaaggc aagggcaagg ttcacggtcg tcatgctctt gccgacgccg
    10081 ccctt
//
LOCUS       DASTQY010000094         3302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_142156, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000094 DASTQY010000000
VERSION     DASTQY010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142156"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="VFR82_03060"
     CDS             <1..1236
                     /locus_tag="VFR82_03060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU4500606.1"
                     /translation="ITEAEEAQAWGITFDKPKLDLEALRHRMQAIVGKLTKGVQTLAA
                     SRKVEVVHAKASFKNATTLSLSGTGGHRDLSFAHAILATGSRPAIPAALKVDDARVMD
                     STGALELPDVPARLLVIGGGYIGLEMGTVYQALGSEVTVVEALPHLLNGADPDLVRPL
                     QLRMQRRFKDIRLNTKVEKLDARKDGIGVTSTGPDGNTTDLFDRVLVAVGRKPNTEQL
                     GLEQTKIALSPKGFIQVDKQMRTAEQTIFAIGDVVGEPMLAHKASHEGLVAARVIAGQ
                     QAAFDATIPAVIFTDPEIAWCGLTEEAAKTSGQQVTIARFPWAASGRATTLGRNDGLT
                     KLVLDAESGRVLGVGICGAGAGELIAEGVLAVEMGAMAEDVAASIHPHPTLSETVMEA
                     AETFHGQATHLFTSKTSKR"
     gene            1241..1996
                     /locus_tag="VFR82_03065"
     CDS             1241..1996
                     /locus_tag="VFR82_03065"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEU4500607.1"
                     /translation="MRAFRLLDLTLPSPIENLALDEALHDELDDVGGDPVLRFWESDR
                     HFVVLGPSCRRMDDVHIDTCEADGVPVLRRASGGGTVLQGPGCLSYALILPLTLHPQL
                     ANIRSTNQFILERIAEAVHGWEPGTVFVGISDLAIDRRKISGNAQRRKRNALLFHGTI
                     LYAMQGDLVARYLKQPARQPEYREDRPHATFLRTIDASPVDLKHAIAKAWGADTPLDV
                     WPQARMAESIRNVLRRSEESMHVAELPFGLAAS"
     gene            2022..2261
                     /locus_tag="VFR82_03070"
     CDS             2022..2261
                     /locus_tag="VFR82_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500608.1"
                     /translation="MKKLDSRSRVTKKTVPAAKSRDTTADLRQQLEDLTEKFDLVLPE
                     VTARVAALEHLLREKNLVSRHDLISARAFVRLQEE"
     gene            2594..3115
                     /locus_tag="VFR82_03075"
     CDS             2594..3115
                     /locus_tag="VFR82_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500609.1"
                     /translation="MNMFHAGAAVLLCGTLASCATNPLTQTTTMARPSIGDFTDHVLE
                     PLALRVADEKVIEATARLKLARAPRGTVQTFATLLYEVPWVGKVLSFRNATQKAQLEE
                     DLAWLETRRIPLKRELLDLFVSRTTYEEQTFTFCVDGVQRRYQALDTNRFTRLSDGPG
                     PCEPMEFSSLRKT"
BASE COUNT          675 a          986 c         1006 g          635 t
ORIGIN      
        1 atcacggaag cggaagaggc gcaggcctgg ggcattacct tcgacaaacc caagctcgat
       61 ctggaggcct tgaggcaccg gatgcaggcc atcgtcggga aacttaccaa aggcgtgcag
      121 actctggcgg ccagccgcaa ggtcgaggtg gtccatgcga aagcctcgtt caagaatgcg
      181 acgacgttga gcttgtccgg taccggcgga caccgcgacc tgtcgttcgc ccatgcgatc
      241 ctggccaccg gttcacggcc ggcgattcca gctgctctca aagttgacga cgcgcgcgta
      301 atggattcga ctggcgcgct cgaactgccg gatgttccgg cgcgtctgct ggtcatcggc
      361 ggcggctaca ttggtctgga aatgggcact gtctaccagg cactcggttc cgaggtgacg
      421 gtggtcgaag ccctgccgca tctgctcaac ggtgcggacc ccgatctcgt gcgaccgctg
      481 caactgcgca tgcaacgacg gttcaaggac atcaggctca acaccaaagt cgagaagctc
      541 gatgcccgca aggacggtat cggcgtgact tcaacaggtc cggacggcaa caccaccgac
      601 ctgttcgatc gcgtgctcgt cgctgtggga cgaaaaccca atacggagca gctcgggctc
      661 gagcagacaa agatcgcgtt gtcccccaaa ggcttcatcc aggttgataa gcagatgcgg
      721 acggccgaac agacaatctt cgcgatcggc gatgtggtcg gcgaaccgat gctcgcacac
      781 aaagcctcac acgaaggatt ggtcgccgcc cgcgtcatcg ccgggcagca ggcggcgttc
      841 gatgcgacga ttccagccgt catctttacg gacccggaaa tcgcctggtg cggacttacc
      901 gaagaggcag ccaagacctc cggacagcaa gtgacgatcg cgcgatttcc ctgggccgca
      961 tccgggcggg ccaccacctt gggacgcaat gacggcctga cgaagttggt gctggatgcg
     1021 gagtctggcc gcgtgctcgg tgtcggcatc tgcggggccg gtgcgggtga actgatcgcc
     1081 gaaggcgtgt tggcggtcga aatgggcgcg atggcggaag atgtggccgc gtcgatccac
     1141 ccgcatccga ccttgagcga gacggtgatg gaagcggccg agacctttca tggacaggcc
     1201 acccacctgt tcacgtccaa gacgtcgaag cggtaacgcc gtgcgtgcat tccgactgct
     1261 cgatctgacc ctcccctccc ccatagaaaa tcttgctctg gacgaagcct tgcacgacga
     1321 actggacgat gtcggcggtg atccggtcct gcgcttttgg gagagcgacc gtcacttcgt
     1381 cgtgctgggt ccgtcctgcc ggcgcatgga cgatgtccat attgacacgt gcgaagcaga
     1441 tggagtcccc gtgcttcggc gtgcgagcgg cggcgggacc gtcctgcaag gacccggctg
     1501 tctgtcatat gcgctcatcc tgcccctaac gctgcatcct caacttgcaa atatccgcag
     1561 cacgaatcaa ttcatcctcg aacggatcgc cgaggcagtc catggatggg aaccgggtac
     1621 ggtttttgtg gggatcagcg atctggcaat cgacaggaga aaaatttcgg gcaacgctca
     1681 acgacgcaag cggaatgccc tgctcttcca tggcacgatc ctctatgcga tgcagggcga
     1741 tctcgtcgcg cgctatctga agcaaccggc acggcaaccg gagtatcgcg aggaccggcc
     1801 gcatgctacg ttcctccgga ctatcgacgc ctcgccagtg gatctcaaac acgcgattgc
     1861 caaggcgtgg ggcgccgaca caccgcttga tgtgtggccc caagcccgga tggcggagag
     1921 catccggaac gtgctacgtc ggtcggaaga gagtatgcat gtcgctgaac tgcccttcgg
     1981 cctcgccgca tcctaagcct ttacgaggag gaatcacggt catgaagaag ctcgattccc
     2041 ggagccgtgt gacgaagaaa acggtgcccg ctgccaagtc gagagatacc actgcggacc
     2101 tacgtcagca attggaggat ctgaccgaga agttcgatct ggtccttccg gaggtcacag
     2161 cccgtgttgc cgcgctcgag catctgctcc gtgaaaaaaa tctggtcagt cggcatgact
     2221 tgatttccgc gcgggcgttt gtccgccttc aagaagaatg agcggagtac cgcccacgct
     2281 ggtcctgtgc tccccgagtg cgcacgcggg gttgatgaga tttatacatt gagggcggtg
     2341 ctccctcgac gggcgcagtg ggaccagcat gaggcgtcac tccttgaaag agcatgagtg
     2401 agcaggagga cctcgtccaa gcgcgcggtg ggagaccaat cattctcccc cttgggacgc
     2461 agcaggagtc cacccggccg tcaccaagcc cgtcattagg gacgtaccca gtcggcatgc
     2521 ttgaggtctt gccgttatac tgacgcgctc atgcttttag attgatggtc accatggccc
     2581 aaggagattc tcaatgaaca tgtttcacgc cggtgcggcg gtcctgctct gtgggactct
     2641 cgcttcttgt gcgacgaatc cgctcacaca gaccacgacg atggcgcgcc cgtccatcgg
     2701 agactttacg gaccatgtct tggagccgct tgccttacgg gtcgccgatg agaaggtcat
     2761 tgaagccact gcgaggctca aattggcacg tgcgcccaga ggcaccgtgc aaaccttcgc
     2821 tacccttttg tatgaagttc cctgggtggg aaaggtgctg agctttagga atgcgaccca
     2881 aaaggcgcaa ctcgaggagg atctggcgtg gctcgagacc agacgcatcc cactgaaacg
     2941 tgagttattg gatctgttcg tttctcggac gacctacgag gaacagacat ttacattctg
     3001 cgtcgacggc gttcaacgcc gctaccaggc actcgatacg aaccggttca cccgcctgtc
     3061 agatggcccc ggtccttgcg agccaatgga gttcagcagc ttgagaaaaa cctaaaggtg
     3121 ttagtgaaca aagagacggg aagccagcga gttcacacat tactgctagg cggcgccggc
     3181 caggtagacc ggcgccacta cctgtccgtc ccatactgat ttagcgactg ctctgtcctg
     3241 acttcgcccc ctgtttgccg ctggactgtg gtccggtttt gcctgatccc gtgccggagc
     3301 tc
//
LOCUS       DASTQY010000095         3767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_341816, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000095 DASTQY010000000
VERSION     DASTQY010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341816"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VFR82_03080"
     CDS             <1..565
                     /locus_tag="VFR82_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500610.1"
                     /translation="ARMEYNETPRTDIFTQGCRDLIRTFTYEAIVACTVYLPPMLFMV
                     GPAAIVTQHPLVLIVLQSVLGFGMSWSIHRMGHCLLKALPKTPQKIYLCGALCLPVRL
                     TLSQFIWMMEVLVSAGVFFGIIAKRLLAEDLASSLLLFGFGIMLYFTPVYLTRLWTQR
                     YYPAMTLVRPTEDVIRKSFPGLRSLFQ"
     gene            809..1138
                     /locus_tag="VFR82_03085"
     CDS             809..1138
                     /locus_tag="VFR82_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500611.1"
                     /translation="MKTKFIGLAVRAVVTILFGASIVSAETAMVTKLDSKPGPAHQTI
                     QGKLSGIEGNTYVVEQSVDDYRGESVTQEVRVYVGKETKRLGGHKKVGDKIRVEVTRG
                     GFANAIQ"
     gene            1513..1977
                     /locus_tag="VFR82_03090"
     CDS             1513..1977
                     /locus_tag="VFR82_03090"
                     /inference="COORDINATES: protein motif:HMM:NF022458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HEU4500612.1"
                     /translation="MKSLIGTAALALCFAPTLLGAAWAALGEPAASVERDRVMMKGQR
                     QSRSGTGYSIDTITVAGIQVKEYVSPDGVVFAVVWTGTGAPDLQLLLGEYFEEYRDGV
                     NAARSHRPKIRKPFQLKSQRLVVERGGHSRSLWGRAFLPAALPPGVTAEDIR"
     gene            1974..3143
                     /locus_tag="VFR82_03095"
     CDS             1974..3143
                     /locus_tag="VFR82_03095"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 domain-containing protein"
                     /protein_id="HEU4500613.1"
                     /translation="MRWVAAFSLVVCLLAGCSNGDDDSNAPSSAGNVVPVSVGESAVC
                     RTINQLCAAITLCQPGTSNCQTVSDVLVDNGSVGLRVFSSVLSTPLTQTVDVQGRAVG
                     ECTFFADGGTTWGPVQVADVVLGGAPAVRVPIQVIQSTFGGQSSSSNPCNDEVDIDPE
                     VASFNGILGIGIFRQDCGLVCAVNSNNTLYFSCNGGSCTGVAVPLANQVQNPVWLLPS
                     GNNGLVITLPNVSAMGAPSVSGSLILGIGTAVNNTPPSGISVLTTDDNGFITTVYKGR
                     TFSQSIIDSGSNGLFFPDTSLRQCAAPLDPFYCPPSAVNLSGTMIGTNGGQIVVPFQV
                     ANTDNLVQTGNAAFNNLGGDFSIFDWGLPFFFGRTVFIGIEGQSSSLGIGPYFAF"
     gene            complement(3504..3713)
                     /locus_tag="VFR82_03100"
     CDS             complement(3504..3713)
                     /locus_tag="VFR82_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500614.1"
                     /translation="MEETIELLFGNRFFQLPFLEALSYIAAMLLIGATIVGLTRRLMG
                     QSAKPAETTYHQPTYSESYEWRKAA"
BASE COUNT          755 a         1053 c         1090 g          869 t
ORIGIN      
        1 cgctcgtatg gaatacaacg agacgcctcg gactgacatc ttcacgcaag gctgccgtga
       61 cctcattcgg acgttcacgt acgaggcgat tgtggcttgt actgtctatc tcccgccgat
      121 gctcttcatg gtgggacccg cggcaattgt cactcagcat ccgttggtcc tcatcgtcct
      181 ccagagtgtc ctcgggtttg ggatgagctg gagtattcac cgtatgggac actgcctact
      241 caaggcgctg ccgaagacac cgcagaagat ctacctctgc ggggcgctgt gcctgccggt
      301 gaggctcact ctcagccagt tcatttggat gatggaggtc ctcgtgtcgg cgggtgtctt
      361 tttcggaatc atcgccaagc ggctgcttgc cgaggatctt gcgagcagcc tcttgctgtt
      421 cgggttcggc atcatgctgt acttcacacc agtctacctg acgaggctct ggacccagcg
      481 atactatccc gccatgacgc tcgtgcggcc gacggaagat gtcatcagaa agtccttccc
      541 gggcctgcgc tccttgttcc agtaagcacg ccagttcccc gcctctatgc ccgcggaatc
      601 cgatccgaca gacgagattc gacgatcgtt tgacgttttt tggtcctttg gctgcggcat
      661 caccgctgag acccaggctt tagagtgcaa ccccgcgaga aaccgcatag tccatttcag
      721 cagtgaccgg tgttctcagg aacaggcctt gctgaatagg ctgcttcggt tcaacgagga
      781 tataaacgct gtgactaagg aggttgcgat gaaaacgaaa ttcattggac tggcggtccg
      841 ggctgttgtg accattctgt tcggtgcgag catcgtctcg gctgagacgg caatggtgac
      901 gaaattggat agcaagccgg gcccagcgca tcaaacgatt caaggcaagc tctccggcat
      961 cgaaggcaac acctacgtcg tagaacaatc agtcgacgat taccggggcg agagcgtgac
     1021 acaggaagtg cgggtgtatg tcggaaagga aacgaagcgg cttggaggac acaagaaagt
     1081 cggggacaag atccgggtcg aagtgacgcg gggcgggttc gcgaacgcca tccagtaagc
     1141 ctccaggcat ctgctcaggc aaaacggatg tgaacttcct gatgcccgct tctcatcgaa
     1201 gcgggcgtgc gcttctcaac ccagaatccc gagcttctca ctctaccggc acctcacaca
     1261 cgcccatccc aacaatttcc atccatgaag cacggtcttt ccccccttcc atgggatgga
     1321 caaggtcatt cgacaatcgg catgatggcc aaaaatctca ctactgccgc gcagctgcca
     1381 caactggtgt gggtggtact gcggacatgg tcgcgggcta gggtccaagg cctggaaagc
     1441 tagggagccc ctcaatgggg cgttgtcctt aatgcgtctt agctatgatg agtctcactt
     1501 ggaaagccgt cgatgaagtc tctgattggc acagctgcgc ttgccctgtg ctttgcgccc
     1561 acactgcttg gagcggcctg ggccgcgttg ggagaaccgg cagcctccgt ggagcgcgac
     1621 cgcgtgatga tgaagggcca gcggcaaagc cggagcggga cggggtattc gatcgatacg
     1681 atcacggttg ccgggataca ggtcaaggaa tacgtctcgc ccgacggtgt tgtgttcgcc
     1741 gtcgtctgga caggcacggg tgcaccggat ctccagctcc tgctcggcga gtatttcgag
     1801 gagtaccgcg acggagtcaa tgcagctcga agccaccggc cgaaaatacg aaagcccttt
     1861 cagttgaagt cccagcggtt ggtcgtggag cgtggtgggc attcccgttc cttatgggga
     1921 cgagcgtttc tcccggctgc cttacctccc ggggtcacag cagaggatat tcgatgagat
     1981 gggtggcggc tttcagtctg gtcgtgtgcc ttcttgccgg ttgttcaaac ggagacgatg
     2041 acagcaatgc cccgtcatcg gcgggcaatg ttgtgcccgt atccgttggt gaatccgccg
     2101 tctgtcgcac gatcaatcaa ctctgcgcag ccatcacgct ttgccagcca ggcacctcca
     2161 attgccaaac cgtttcggac gtgctggttg ataatggatc ggtaggcctg cgtgtgttta
     2221 gctcggtatt gtccacgccg ctcacacaga cggtggatgt ccagggtcgc gcggtcggcg
     2281 aatgcacatt ttttgcggac ggcgggacga cgtgggggcc cgtgcaggta gctgatgtgg
     2341 tattgggtgg cgcgccggcc gttcgcgttc ccattcaagt gattcaatcg accttcggag
     2401 ggcagtcatc gtcgagcaat ccgtgcaacg acgaagtgga tatcgacccg gaggtggcga
     2461 gctttaatgg gattctcggc atcggcatct ttcgccagga ttgcggattg gtctgtgcag
     2521 tcaacagcaa caataccctg tacttttcct gtaacggtgg ctcctgcacc ggtgtcgcgg
     2581 ttccgctagc taatcaagtg caaaatcccg tgtggctgct cccctccggt aacaacggcc
     2641 tggtcattac tctacccaat gtttccgcca tgggagcgcc gtccgtctcc ggttccctga
     2701 ttctcggaat cgggactgcc gtcaataaca ccccgccgtc tggtatatcg gtcctcacta
     2761 ccgatgacaa tgggttcatc acgacggtgt acaaaggcag aacgttcagc caaagcatca
     2821 tcgattctgg ctccaacggc ttgttctttc cggacacaag cctgcggcag tgtgccgccc
     2881 cgttggatcc attctattgc ccgccaagtg cggtcaatct ctcagggaca atgatcggaa
     2941 cgaacggggg ccagatcgtc gttccgtttc aagtcgccaa cacggacaac ttggtgcaga
     3001 caggcaacgc ggctttcaac aatttggggg gagacttttc gatcttcgat tgggggcttc
     3061 cgttcttttt tggccgtacg gtattcatcg ggatcgaagg acaatcgagc agcctgggta
     3121 tcggcccgta cttcgccttc taatgattcg acagtgcgaa ggcgactagt gcatttctag
     3181 ctgcgcgact ggtcattttc ccaggatcag cttctcagct gatgcggtaa ggtgaaatta
     3241 ctttagaaaa cggcagtgga aacattgccg catctccgag gtaggagccc gctgccgaaa
     3301 ggtagcgggc tctttttttc ccccctacat cctatcgagg attgcccggt atctgtgcgt
     3361 tagtcggcat ctgtgtgtta gatggagact gattggtctc tcattgtggg caacgtcctc
     3421 acgcctcccg cctagcgact gctgcgcact cgtttcgccc ctgcagtacc ggcgagcatg
     3481 agagaccaac agccttcttt tcgctaagcg gccttacgcc attcgtaaga ctcgctgtag
     3541 gttggctgat gatacgtggt ttccgcgggt ttcgcggact gccccatcag gcgcctcgtc
     3601 aggcctacga ttgtggcacc gatgagaagc atggcggcga tatacgacaa tgcttctaga
     3661 aacgggagtt gaaaaaatcg attgccaaat aacaattcga tggtctcttc catcgtatac
     3721 ctccttgtgt gctggttttg cctcgatgcc cgagactgtg aatcaag
//
LOCUS       DASTQY010000096         2682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1147327, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000096 DASTQY010000000
VERSION     DASTQY010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147327"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFR82_03105"
     CDS             <1..485
                     /locus_tag="VFR82_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4500615.1"
                     /translation="LMDVRLPDGSGVEACREILSEQPNSRVIFLTSYTDDDSVLAAVL
                     AGAYGYVLKEIDSAALIRAIRTVSTGRSILDPAVTKRALEWIKTVPTAEGPVRPSSLS
                     PQEERVLALVADGLTNKEIATSLKLSDKTVKNYLANMFQKLHISRRAQAAAFFVKRTV
                     "
     gene            557..973
                     /locus_tag="VFR82_03110"
     CDS             557..973
                     /locus_tag="VFR82_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500616.1"
                     /translation="MASSWTLRINAVLGSLVVATGAWLAWEALAGVGGLMILVIAGCF
                     LLWRGRTIRVIWAWSTLFLGIECLAWPIITMLRIRSATAQPSDEEMGTILSAVLMGLF
                     SAVFWIAFSYGLFKGTDPDRLPQSAQMQSPRRHKKR"
     gene            complement(978..>2682)
                     /gene="priA"
                     /locus_tag="VFR82_03115"
     CDS             complement(978..>2682)
                     /gene="priA"
                     /locus_tag="VFR82_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU4500617.1"
                     /translation="TSSSSDLWCAEGDQHKDALQGMDPSLQDWNARVDQALGKREAAR
                     LLVEAASGERLSLLRYAVDRTVALGRRALVITGETARAESLTLALHNPTRVTTCLHSA
                     IPDEKKADIWNLIREDKVSVVVGTRSAVFLPLHSIGLIWIDREEDPALKEPMEPRYHA
                     RDVAWKRAREEQALLVLSSAHLSLEATAQAPQHVLRAPVRREATPHIEVVDLRRHDRT
                     GLLSPTLIQAMREAIVRQAGLLLFLNRKAYAGALVCRDCGQVPRCRSCTVALAYSRQK
                     GSVFCHYCGVADAIPDLCPACGGPRLQPVGEGTERLEEEVKRQFPLVRVLRVDGETMR
                     RAKDAAAMWNRIHRREWDVLIGTQLLLRDDVVPPVGLLGVVQADAGLSVPDFRAAERT
                     YHLLLDASRLVEPRGTGGRLIIQSYLPTHHVIEAVVQQEAGRFRTEELAHREALGFPP
                     LLRLIVLHISGPAERSVEQAAQAWATRLRGLTTVARGASQDREAVRAIGQTTGLTVLG
                     PVLSPIARVRGRYRRQILVKSADGASAVEAVRSTLADLETTYPPRQVKFDVDVDPIDM
                     W"
BASE COUNT          518 a          832 c          800 g          532 t
ORIGIN      
        1 ttctgatgga tgtccgcctt ccggacggct ccggcgttga agcctgtcgc gagatcctct
       61 ccgaacagcc caactcgcgc gtgatcttcc tgacatctta cacggatgac gattccgtcc
      121 tggccgccgt cttggcgggc gcatacgggt acgtgttgaa ggagattgat tccgcggcgc
      181 tgattcgcgc cattcggaca gtctccacgg gccgctcgat cctggatcct gcggtcacga
      241 agcgcgcgct cgaatggatc aagacggtcc cgacggccga aggtccggtt cgaccgtctt
      301 ccctctcgcc tcaggaagaa cgggtgttgg ccttagtcgc cgacggctta acaaacaagg
      361 aaatcgcgac atcgctcaag ctgagcgaca agaccgtcaa aaactatctc gccaacatgt
      421 ttcaaaaact ccatatctcc cgtcgtgccc aggcggccgc attttttgtt aagcgcaccg
      481 tgtaagcggg cccctcacag atccggccac cgacgagccg gcacccccac caccgacgag
      541 tacaatgggc gagctcatgg catcatcgtg gacacttcgc atcaatgcgg tgctcggaag
      601 cctcgtcgta gcgacaggcg cctggcttgc ctgggaggca ttagccggcg tgggcgggct
      661 gatgatcttg gtgatcgccg gatgctttct cttgtggcgg ggccgcacca tccgtgtgat
      721 ctgggcatgg tccacccttt tccttggcat cgaatgcctg gcgtggccaa tcatcaccat
      781 gctgcgcatt cgatcggcga cggcccaacc gtcggatgag gagatgggca cgattctctc
      841 ggctgtgctc atggggttgt tctcggcggt cttctggatc gccttctctt acggcttgtt
      901 caaaggcact gatccggacc ggctgccgca atccgcacag atgcaatccc ctcgtcggca
      961 caagaagcga taatgcctta ccacatgtcg atcggatcga catcgacgtc gaactttacc
     1021 tgtctcgggg gatacgtggt ctccaggtca gcgagggtcg atcgaacggc ctcgacggcg
     1081 ctggccccgt cagcggactt cacaaggatt tgtcgacgat agcgcccccg cacgcgcgcg
     1141 atgggggaca ggaccggtcc caataccgtc aggccagtcg tctggccgat ggcacgaacg
     1201 gcttctctgt cctgtgaggc gccgcgtgca accgttgtaa ggcctctcag ccgggtggcc
     1261 caggcttgcg ccgcctgctc gacgctgcgt tccgccgggc cggagatgtg gaggacaatc
     1321 aagcgaagca agggaggaaa gcccagcgct tctctgtgcg ccagctcttc ggttcggaag
     1381 cgtcccgctt cttgttggac gacagcctca atgacgtggt gtgtgggaag ataggattga
     1441 ataatgagac gtccgccggt tccacgaggc tctacgagtc gggacgcgtc cagtaagagg
     1501 tgataggtgc gttcagccgc tcgaaaatct ggaacgctga gcccggcatc ggcttgcacg
     1561 acgcccagca ggccaaccgg cggcaccaca tcgtcccgca agagtagttg tgtcccgatg
     1621 aggacatccc actctcgccg atgaatccgg ttccacatcg cagcagcgtc cttcgctctt
     1681 cgcatcgtct ctccgtcaac ccgcaggacg cggaccaaag ggaactgtcg cttcacttct
     1741 tcttccaacc gttccgtgcc ttccccgacg ggttgcagtc gtggcccgcc gcaagcaggg
     1801 cagagatcag gaatcgcatc cgccactccg cagtaatgac aaaacaccga gcccttttgc
     1861 cgtgaatacg cgagtgcgac ggtgcaagat cgacagcggg gaacctgacc gcaatctcgg
     1921 cagaccagcg cgccggcgta cgcctttcga ttgagaaaca acaggaggcc cgcctgccgc
     1981 acgatcgctt cccgcatggc ttgaatcagg gtggggctta aaagacccgt gcgatcgtgg
     2041 cggcgaaggt caacgacttc aatgtgtggg gtagcctccc gtcgaacggg tgcgcgcaac
     2101 acatgctgtg gggcctgtgc cgtggcttcc aatgagagat gagcggaaga caatacaagg
     2161 agcgcctgtt cctctcgggc tctcttccag gccacgtcac gggcatgata tcgaggctcc
     2221 atcggttcct tgagggcggg atcctcttct cggtcgatcc agattaatcc gatcgagtgg
     2281 agtggcagaa acaccgcaga tctggtaccg acgacgaccg agaccttatc ttcgcggatc
     2341 aaattccaga tgtccgcctt cttctcgtcc gggatcgcgc tgtgcagaca ggttgtcaca
     2401 cgcgttggat tatgaagcgc gagcgtgagg gattctgcgc gcgccgtttc accggtgatg
     2461 acgagcgccc tacgcccaag agcgaccgtt ctgtccaccg catagcgaag cagagaaagg
     2521 cgttcgcccg aagcggcttc gacgagcaga cgggcggcct ctcgtttacc gagagcctgg
     2581 tccacgcgcg cattccagtc ctgaagcgaa ggatccatgc cttgcagggc atccttgtgt
     2641 tgatcaccct cggcacacca gagatccgag ctgctcgacg tc
//
LOCUS       DASTQY010000097         3343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1752651, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000097 DASTQY010000000
VERSION     DASTQY010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752651"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFR82_03120"
     CDS             complement(<1..183)
                     /locus_tag="VFR82_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4500618.1"
                     /translation="MSGGLSISGIALITGANKGIGLEVVRQLATRGWRVFLTGRSLAS
                     VRKAASPLGPSVTPLPL"
     gene            324..815
                     /locus_tag="VFR82_03125"
     CDS             324..815
                     /locus_tag="VFR82_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500619.1"
                     /translation="MGPRMLVIGMGLFLGWSIAGCANVQVLRLTSETFPAREVEDVAI
                     LSQFPGDQHQKVAELSETSSSENVSKLQRHLLDKAAELGADAVVFSTPITRIEQRVAY
                     QSVYSPWGYYAPYYGPGPYGYGGPWGYRYGPWGPMLGYHQAVPVPYQVRSTTVKGTAI
                     RYS"
     gene            857..1351
                     /locus_tag="VFR82_03130"
     CDS             857..1351
                     /locus_tag="VFR82_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500620.1"
                     /translation="MKIHCMWMIGLAILAAAGCNGTVGTERVAAERHIHSRPDPVLNM
                     LHRGVIELNESIDELKRDIADLQQLPAVSDPQVQQLHGLDLAAWQLHLQQWVVQRDRL
                     LSSLNFIQQAQAAPQDKAAIGDRWSERQAEFIKVIEELRAQRRTIEQKRSDVESQVLG
                     RYFN"
     gene            1537..2040
                     /locus_tag="VFR82_03135"
     CDS             1537..2040
                     /locus_tag="VFR82_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500621.1"
                     /translation="MRNPRVSSWQLYSGCLILAGLLSGGCGLRASPVQMYEGPSLPKE
                     QVGIVRSACKTGSGLTIMIVRVDDKDVRNTCADFALLPGDHNLELSAKRLAPRIDTPM
                     IRSGSATGPPPSTIGGNQDEDLPVIWASSSPLRISCTLRPGQEVTIIGNAGTGPDWEA
                     RCQERAR"
     gene            complement(2172..2900)
                     /locus_tag="VFR82_03140"
     CDS             complement(2172..2900)
                     /locus_tag="VFR82_03140"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4500622.1"
                     /translation="MTVTNRQSGTNVHEVAEGIYRINTPVVIEGAGGFSFNQYLIVDE
                     EPLLFHTGPRKMFPLVREAVTSVVPIDRLRYIGLSHVEADECGSLNEWLGAAPQSVPL
                     CGTVAALVSIGDLADRMPRALADGELLPLGRHTVRWFDAPHLPHAWECGYLIEERTST
                     LLCGDLFTQGGAELPPLTESDILGPSEGFRQQMDYFSHTKNARAMLERLAATHPRTLA
                     CMHGSAWHGDGANLLRALADSLSA"
BASE COUNT          717 a          973 c         1012 g          641 t
ORIGIN      
        1 gagggggagc ggggtgaccg aggggccaag cggggaggcc gctttccgca ccgaggccag
       61 cgagcgcccc gtcagaaaca cgcgccatcc gcgcgtcgcc aactgccgga cgacctccag
      121 cccgatgcct ttattggcgc ccgtgatgag ggcgatgccc gagatcgaca agcctccact
      181 catccctcat tataggatct ctcggaaaaa taccggctgg gcttgtccct agaatacggc
      241 gaaaagggag aggtggccgt cgcaccatgc gcctgtcgtc gcacctagca gacacatggc
      301 gtaggacagg ctaggatgcc atcatgggac ctcgaatgtt ggtcatcggg atgggtctgt
      361 tcctcggctg gagcatcgcc ggctgcgcga acgtgcaagt gcttcggctc acctcggaga
      421 catttccggc tcgcgaggtg gaggacgtcg ccatcctcag ccagtttcct ggcgaccagc
      481 atcaaaaggt cgccgaactc agcgagacgt cctcgtcgga gaacgtttcc aaacttcaac
      541 gacatctcct cgacaaggcg gctgagctcg gggccgacgc ggttgtgttc tcaacgccga
      601 ttacgcggat cgagcaacgc gtggcgtatc agtcggtcta tagcccatgg ggatactatg
      661 caccctatta tggtccgggt ccctacggct atggggggcc gtgggggtat aggtatggac
      721 catggggacc aatgttgggc tatcaccaag ccgtcccggt gccgtatcaa gtgcggagca
      781 cgaccgtgaa aggcacggcg attcgctata gctgacgacg attcaatagg tgcccgctca
      841 gtcggaggac aaaactatga agattcactg catgtggatg atcggactgg ccatactggc
      901 cgcggccggc tgcaacggaa ccgtcgggac agagcgggtc gcggcagaac gacacataca
      961 ctcgcggccg gatcctgtcc tcaacatgct gcaccggggc gtgatcgaac tcaacgagag
     1021 tatcgacgag ctgaagcgcg atatcgccga cctccagcag ttgccggccg tctcggatcc
     1081 acaagtgcaa cagctgcacg gattagatct cgcggcctgg cagcttcacc tccagcaatg
     1141 ggtggtccaa cgcgatcgtc tcctgtcctc gctcaacttc attcagcagg cgcaggcggc
     1201 tccgcaagac aaggccgcca tcggcgatcg atggtcggag cgccaggcgg aattcataaa
     1261 agtgatagaa gagttgaggg cccagcgacg aacaatcgaa cagaagcgct ccgacgtcga
     1321 atcgcaagtc ctggggcgtt atttcaatta ggcttcgaac cgaactgctc gtcgcagcgg
     1381 cgatcactga gcagggcgcc ttttaagaga gcccttcccg tcatgctacg ggaaaagtag
     1441 aatgtccctt tctccttccc gactcaaatg gcatttctga gctgacagaa gcgcttcttc
     1501 gtgtcctcgc tcaagagcga ggggagagag gtacacatgc gaaatcccag agtgtcgtcg
     1561 tggcaactct atagcgggtg tctcatcctg gctggactcc tcagtggcgg ctgtggcctc
     1621 agggcgagcc ccgtccagat gtatgaaggc ccgtcgcttc ccaaggagca ggtcggcatt
     1681 gtgcgcagtg catgtaagac cggatccggc ttgacgatca tgatcgtgcg agtcgacgat
     1741 aaggacgtcc ggaacacctg tgcggatttc gcgctgctgc cgggtgacca caatcttgaa
     1801 ctcagtgcca agcgactggc gccaaggatc gatacgccca tgatccggtc cgggtcagca
     1861 acggggccgc cgccgtctac gataggtggg aatcaggacg aagacttgcc ggtcatctgg
     1921 gcatcctcat cgccgctgcg aatcagttgt acgctccggc caggacagga agtcaccatc
     1981 atcgggaacg caggcacggg gcctgattgg gaagcgcggt gtcaggagcg ggcacgctag
     2041 acatggcacc ctttgcctta ggcagaaagc gaaggggatt ctgatgacct cggcgtgcac
     2101 tcaaagacgg acggcaagag ctcgaacgcc gtggcggatg atctcgaccg aacgcccagt
     2161 tgagcgacag ctcatgccga gagcgaatcg gcgagcgcac gcagcaggtt tgccccatca
     2221 ccatgccagg cgctgccgtg catgcacgcg agcgtgcgcg ggtgtgtcgc ggcgagccgc
     2281 tccagcatcg cacgggcgtt cttcgtatgg gagaagtaat ccatctgctg ccggaacccc
     2341 tcgctcgggc caagaatatc tgactccgtg aggggcggaa gctcagcgcc tccctgcgta
     2401 aacaagtccc cacagagcaa cgtcgaggtg cgttcttcga tcagatagcc acattcccat
     2461 gcgtggggca gatgcggtgc gtcgaaccac cgcacggtgt ggcgacccag cggtaacagc
     2521 tctccatcgg cgagggcacg gggcatccga tcagcgaggt cgccgatcga gaccagggcc
     2581 gcgacagtcc cgcacaacgg cacagactgt ggagcagcac caagccactc gttgagtgaa
     2641 ccgcattcgt cggcttctac atgggacagt ccaatgtacc gaagcctgtc gatcggcacc
     2701 acactcgtca cggcttctcg cacgagcgga aacatctttc gcgggccggt atggaagagc
     2761 aatggttcct catcgacgat gagatattgg ttgaacgaga agccgcccgc gccttcgatc
     2821 acgaccggag tgttgatgcg atagatccct tcagcgacct catgcacgtt cgttccggac
     2881 tgcctgttcg ttacggtcat cggcgtctcc ttgtcatggg ttacgcgctg gtcgcgctcg
     2941 ggtgtctgac gttcccatgg acataagatg cacgacatag actcagggtt gcaatgcaac
     3001 tgctgaattt caaaacaaca aagggtttct ttctctttct gcttcatgaa tttgtctctt
     3061 ctctctcgca gagatccgtg ccagcgactg accctcgacc gcccattcaa gttatgaaaa
     3121 gaacaggcgt tgttggtgca attgcgtgac tgcagctgcc cccttgtctt tttcttagcc
     3181 ttctgaggaa gtgagcgtgg tcacttaata ggggaggcgg ggacgatcac gaacgagtga
     3241 ggatattgtg ctgagcaccc cgggccagac aagaagtcat catcgccgag cccggggatt
     3301 gaaatcacga caatgttcag tctcttcctc aaaagaatca ctg
//
LOCUS       DASTQY010000098         3906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1146791, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000098 DASTQY010000000
VERSION     DASTQY010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146791"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..1206)
                     /locus_tag="VFR82_03145"
     CDS             complement(256..1206)
                     /locus_tag="VFR82_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HEU4500623.1"
                     /translation="MAEKKIIAVVGATGAQGGGLVRAILNDPSSGFAARALTRKADSD
                     KAKELAKLGAEVVAADLDDVESLKRAFAGAHGAFLLTNFWEHFSPEKEFAQAKNMAQA
                     AKQANLQHVIWSTLEDTRKWIPLSDNRMPTLKGKYKVEHFDAKGEADHLFTDLGVPTT
                     FLLTSFYWENLIYFGLGPKKGPDGKLVLTYPMGDKKLPGIAVEDIGKSALAIFKKGRE
                     FIGKTVGIAGEHLTGAQMASALTKALGQEVRYNDVSPDEYRKFGFPGAEDLGNMFQFK
                     RDFQEAFCGPRNPSIARGLNPSLQTFDTWLAANNSRIPLS"
     gene            complement(1235..1723)
                     /locus_tag="VFR82_03150"
     CDS             complement(1235..1723)
                     /locus_tag="VFR82_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU4500624.1"
                     /translation="MSYPDARYQGQSGEISAVFRSADKKPELTIGSGTAVRYLATGDS
                     THRQFGLYRWDAQPRTPGPKAHYHRTMSESFFVISGLVRLFNGERWIDAKAGDFLYVP
                     EGGIHGFQNDSDVPSSMLILFAPGAPREGYFEALAAKAAGRQFSEEEWREICVNHDNY
                     FI"
     gene            complement(1761..2075)
                     /locus_tag="VFR82_03155"
     CDS             complement(1761..2075)
                     /locus_tag="VFR82_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500625.1"
                     /translation="MSTQTQRNLLRTVVTATALVIMSGGLNIIVMKTLGSFDAATVIL
                     ALYVGFWTFVLGSIGLVLSSLWLFMKGKVMRTDVASEQLEAHEHGPIGLQALDYHRIC
                     AG"
     gene            2737..3783
                     /locus_tag="VFR82_03160"
     CDS             2737..3783
                     /locus_tag="VFR82_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500626.1"
                     /translation="MRCTRCHGSMQHDGDAKGFLGPRGLLSISSWHCLNCRHAVDFIK
                     ESDPLHVVSELPAQPSNQSRKQKGPFIFGLTFLMLAQANLMGCAIPGQPVHDQVVAER
                     TERQKLEKAYTEVQEALTKQAAVSLAERQKLEKSYTDAKEALTKQVAIGHESEGKLAR
                     LQLLSLEKEIQKKELSSKLEEAMLEVVRTKAKLRSLETKAEAVSALAEGEIALKSLKT
                     NAADQENDARSSKAEELLKASGLELKKENYSGALYLTTQAKTLIREDQERSKDREKKA
                     MMAGEVPFALPLPLQAIGQTKVKEGPSLDSKPLFAVQQGTAFVGYSFKGPWVRVRAED
                     GRSGWVNYKLVDGR"
BASE COUNT          897 a         1129 c         1057 g          823 t
ORIGIN      
        1 acaaggcgca gacacgatcg tgtggctcgc gaccgagtcg ccgcagtcca tgaccggctt
       61 attcatccgc gatcgcaaac ccatcccctg gtaacctgga ctcaggcagc aactccaaca
      121 gagatttttg ggaagatggt cgtttcgcaa acaggtcgca agcccgaggc tttcatcgga
      181 ccgccccggt gggaagaaat aaaggggaca agctgagctg tcccccttct accctaggct
      241 agccttgctt gaagattatg acagcgggat gcggctgttg ttcgcggcca accatgtgtc
      301 aaaggtttgc agcgatggat tgaggccacg ggcaatcgac ggattccgcg gcccgcagaa
      361 ggcctcctgg aaatcccgtt tgaactggaa catgttgccc aaatcttccg cgccggggaa
      421 cccgaacttt cgatattcgt ccggggacac atcgttatac cgcacctctt gccccaaggc
      481 cttggtgagc gccgaagcca tctgcgcacc cgtgaggtgc tcgccggcga ttccgacggt
      541 cttgccgatg aactcacgac ctttcttgaa aatcgccaag gcactcttgc cgatatcctc
      601 gacggcgatg ccggggagct tcttatcacc catgggataa gtcagcacga gcttaccgtc
      661 ggggcctttt ttggggccca gcccaaagta aatcaggttc tcccaataga atgacgttag
      721 caaaaacgtc gtcgggacac cgaggtcggt aaacaggtga tctgcttccc ctttggcatc
      781 gaagtgctcc accttgtact tacccttcag cgtcggcatg cgattatcgc tcagggggat
      841 ccacttcctc gtgtcctcca gtgtggacca gatgacgtgt tggagattgg cctgcttcgc
      901 cgcttgcgcc atgttcttgg cctgagcgaa ctctttctcc ggcgagaagt gctcccagaa
      961 gttcgtgagg agaaacgcac cgtgtgcgcc ggcgaacgcc cgcttgagcg attccacatc
     1021 atccagatcg gccgcaacca cttcggctcc cagtttggca agctctttgg ccttgtcgga
     1081 atctgccttg cgcgtcagcg cccgggccgc gaagccgctg cttggatcgt tcagaatggc
     1141 acgcaccaac ccgccaccct gtgctccggt ggcaccaact actgcgatga tctttttctc
     1201 agccatagct cccccttccc tcactcgatc atgatcaaat gaagtaattg tcgtgattga
     1261 cgcagatttc cctccactcc tcctcgctaa attgccggcc cgcagccttt gccgcgaggg
     1321 cttcaaagta accttcgcgc ggcgcacccg gcgcaaacag gatcagcatg gatgacggca
     1381 cgtccgagtc attctgaaac ccgtgtatgc caccttctgg aacgtagaga aaatctccgg
     1441 ccttggcgtc aatccaccgc tccccgttaa agagccgcac caggccggag atcacgaaga
     1501 atgattcgga catcgtcctg tggtaatgcg ccttgggccc tggcgtgcgt ggttgcgcat
     1561 cccaacgata caagccgaac tgtcggtgtg tcgagtctcc agtggccaga tagcgcaccg
     1621 cggtcccaga cccgatcgtc aactcaggct tcttgtccgc cgaacgaaag accgcgctga
     1681 tctcgccgct ctggccttga taccgcgcat cggggtagga catgggtaac tccttggtga
     1741 tcgcttacaa gaattccagc tcatcctgcg catattcgat ggtaatccaa cgcctgcaga
     1801 ccaattggtc catgttcatg ggcttccaat tgttcgctcg caacatcagt acgcatgacc
     1861 tttcctttca tgaagagcca tagtgacgag aggacgaggc caatactccc cagcacgaaa
     1921 gtccaaaatc ccacatacag cgccagaatc actgtggccg catcgaagga tcctagcgtc
     1981 ttcatgacga tgatattcaa tcctccgctc atgatcacca gggcagtagc agttacgaca
     2041 gtccgtagga ggtttctctg tgtctgtgtg ctcatggcga ccacctttct cccgacacat
     2101 cggttttgaa gctcgtgggt cgatctctgt catctgctga tacgccttga gcaaatcgtg
     2161 ttccacggga gtaatcagca acttggatca acgaagtcgt tgaaactgga ggaggtgccg
     2221 gaatctcggt cgtagagttg tcgaagaatg gaaatcggaa gtgacgatga agagctgccg
     2281 atccaacggc gcgctgccga caggtcggcg ctccggcagg ttgcccagtt ggatttggaa
     2341 ggagaagtct agattctaaa ttaaacggcc ttggctcgta cctgaacagg tcaactcttt
     2401 ctcgaagttg ccctaaagcg cctgaagctc ccggcactgg tgacgacagg cgagaaggac
     2461 gccgtcgtta caccggcaca cacggagcat ttattgagct gtattcccgc tgccgaagca
     2521 tcggtgtttg agggagttgg gcgtagcccg cacttggaag gcgctgagcg ttcaaccgcg
     2581 acttggtttt acgctcgcta aagtggcggt ttacctcatc catgccttac ttccccttct
     2641 tttgaactac ttcctcctag tctatcgcca attggtgcac tcgatagact tgtattggtg
     2701 agagaaaaaa cccttcgaga aaccaaggag tggattatga ggtgtacacg ttgccacggt
     2761 tcgatgcagc atgacggcga cgctaaaggg ttcctaggcc ctcgggggct tttgtcgatt
     2821 agcagctggc actgcctcaa ttgccggcat gctgtcgatt tcattaagga atccgatcca
     2881 ttacacgtgg tatcagaact gccggcccaa ccttcgaatc agtcgaggaa gcagaaggga
     2941 ccgttcatct tcggattgac attcttgatg ttggcacagg ctaaccttat gggctgcgcg
     3001 atccccggcc agccggtcca tgaccaagtc gtcgccgaac gtaccgaacg ccaaaaactt
     3061 gagaaggcgt atacggaagt ccaagaagca ctcacgaaac aggcggctgt ctcccttgcc
     3121 gaacgtcaga agctcgagaa gtcgtacacg gacgccaaag aggcattgac caaacaagtt
     3181 gccattggac acgaatccga aggaaaactc gccagactcc agttactttc gctcgagaag
     3241 gaaattcaga agaaagaact cagcagcaag ctcgaggaag ccatgttgga ggtggtgagg
     3301 accaaggcca aactgcgtag tttagaaacg aaggccgagg cagtctccgc tcttgcggag
     3361 ggagaaatcg ccctcaagag cctcaaaacc aatgccgcgg accaagagaa cgatgcgagg
     3421 tcgagtaaag cggaggagtt gctgaaggct agcggcctgg aattaaaaaa ggagaattac
     3481 agcggtgccc tctacctcac gacgcaggct aagacgctca tcagagaaga ccaggagcga
     3541 tcgaaagatc gggagaaaaa ggccatgatg gcaggcgagg ttcccttcgc actgcctctc
     3601 ccgcttcagg ccatcggcca gaccaaggtc aaggaaggcc caagtcttga ctccaaaccc
     3661 ctctttgccg tccaacaagg gacggcattc gtgggctatt ctttcaaggg tccgtgggtg
     3721 cgcgtgagag ccgaagacgg ccgcagcggc tgggttaact acaagctggt agacgggcga
     3781 taagcactca caccgccatc ttcataccgg ggcctggatc atctccggca aacacaaccc
     3841 gattccgccc gcaccgcttc ccctcataga gcgcgctatc agcgctggcc acgatcgctt
     3901 cgagcg
//
LOCUS       DASTQY010000099         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_586416, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000099 DASTQY010000000
VERSION     DASTQY010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586416"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /gene="trmFO"
                     /locus_tag="VFR82_03165"
     CDS             complement(<1..1219)
                     /gene="trmFO"
                     /locus_tag="VFR82_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU4500627.1"
                     /translation="MREDIVIIGGGLAGSEAAWQAAHRGAKVTLYEMRPKESTPAHKT
                     GGLAELVCSNSLGSADPQNAAGILKEEMRRHQSLIIKVADQVRVPAGSALAVDRDQFS
                     FLITQALDSHPNVRILREEMTEIPADCVCIVATGPLTSDKLSQAIARLTHSNHLYFYD
                     AISPIVDADSINMDIAFRASRYGKGGDDYVNCPMDETTYQAFYDALLAAEKVQPKAFE
                     QTPYFEACLPIEVMAERGRETMQFGPLKPVGLEDPKTSRRPYAIVQLRTENAHRSCYN
                     MVGFQTRLTYGEQKRVFRMIPGLAQAEFLRYGSLHRNTFINSPQLLHNTLQFKERPTL
                     FFAGQLVGVEGYTDSAAMGGLAGINAVRELDRLPPLTPPPTTAHGCLLSYITTSDPHH
                     FQPMNMNFGLFPPL"
     gene            1353..1427
                     /locus_tag="VFR82_03170"
     tRNA            1353..1427
                     /locus_tag="VFR82_03170"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1385..1387,aa:Val,seq:cac)
     gene            1581..1928
                     /locus_tag="VFR82_03175"
     CDS             1581..1928
                     /locus_tag="VFR82_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500628.1"
                     /translation="MKAKLIAVSLLLIAGLIGCAGRQQAADGDIGYVAPASIYSVMDS
                     TALVYTDVAAGSPLNDNPWRWLGFLLHPIGQGLDYGVNRPIYSLTGSMPYLFGYTSED
                     AMLDSQRRRASQY"
     gene            2036..>2513
                     /locus_tag="VFR82_03180"
     CDS             2036..>2513
                     /locus_tag="VFR82_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500629.1"
                     /translation="MTTDIGERRAALRRALIGLLGCLTVSLFAACETPNPRQNLPPSE
                     SDLVLLKPGTLCQRKTDFLQQHPQSSLTIKPWGTGQELMLPASRSPSHGDESFFFDDD
                     GTLVGALFTFASGLDLDPYSVLRHTLTLLKPTLEFYLSVANFAGKSAMDSSSLYETG"
BASE COUNT          522 a          722 c          721 g          548 t
ORIGIN      
        1 tgagaggcgg aaacagtccg aaattcatgt tcattggctg gaagtggtga ggatcgctcg
       61 tggtgatgta cgaaagcagg cagccatgtg ccgtcgtagg aggaggggta agcggcggca
      121 acctgtcgag ctcacggaca gcgttgatgc ccgcaagacc tcccatcgcg gctgaatcgg
      181 tgtatccttc cacacccacc agttggccgg caaagaagag ggtcggccgc tctttgaact
      241 gcagcgtgtt atgcaacagc tgcggcgagt tgatgaacgt gttgcggtgc agactgccat
      301 aacgcaggaa ttccgcttgg gcgaggccag ggatcatgcg gaaaacccgc ttctgctcgc
      361 cataggtcaa tctcgtctgg aacccgacca tgttgtaaca cgatctgtgg gcgttttccg
      421 tcctgagctg aacgatggca tagggccggc ggctggtttt cggatcctca aggccaacgg
      481 gtttcaaggg accgaactgc atggtttccc ttccgcgctc agccataacc tcgatgggca
      541 agcaggcctc gaaatacggc gtctgctcaa atgccttggg ttggaccttt tccgctgcca
      601 gcaaggcgtc ataaaaggcc tgataagtcg tctcgtccat gggacaattc acatagtcgt
      661 ctccgccttt tccatagcgc gatgcacgga aggcaatatc catattgatc gaatccgcat
      721 ccacgatggg cgaaatagca tcatagaaat agagatggtt ggagtgcgtc agccgcgcaa
      781 tggcttgcga gagtttatcg gaagtcagcg gccctgtcgc cacgatgcag acacagtcgg
      841 ccgggatctc ggtcatttct tcccggagaa tgcggacgtt ggggtgactg tcgagcgctt
      901 gcgtgatcaa gaaggagaat tggtcccggt cgaccgccag cgctgacccg gcagggacac
      961 gcacctgatc cgccaccttg atgatcaatg actggtggcg gcgcatttct tccttgagga
     1021 tgcccgcggc attctgaggg tcggcggatc cgagagaatt tgaacagacg agttcagcca
     1081 gccccccggt cttatgggcc ggcgtcgact ccttcggacg catttcgtag agcgtgactt
     1141 tggcgcctcg atgagccgcc tgccatgccg cttcggaccc ggccaaaccg ccgccgataa
     1201 tcacgatgtc ttcgcgcatc tgagtggaac gctccgcgag agaaagtcga gcaggagagg
     1261 cattgtagga atcgtatttc ggcgatgtca aggcgaagcg gccggcgaat gcattgaacc
     1321 cgcggaaggc tcatgctaga ctccgccccg atgggcgatt agctcagtgg cagagcgctt
     1381 ccttcacacg gaagaggcca caggttcgat acctgtatcg cccaccacct ctcaacccat
     1441 cggaattcct cgcatctcac ccaagaccct gcgttggtgt aaacctgtcg ttttctggac
     1501 agaatgctga acctcggcta cacttccttc agttaattag attggtcatt ggaaccgatt
     1561 tcgggacatg aggtagaggt atgaaagcca aattgattgc agtgagtctg cttttgatcg
     1621 ccggcctgat cggttgcgca ggccgtcagc aagcggccga cggggacatc ggatacgtcg
     1681 ccccggccag catctatagt gtcatggaca gcacagcgct cgtctatacg gacgtggcgg
     1741 ctgggtcgcc gctcaacgac aatccttggc gctggttggg attcctgctg catcccatcg
     1801 gacaagggct cgattatggc gtgaaccgtc ccatctactc gctcaccggc agtatgccgt
     1861 acctcttcgg gtataccagc gaggatgcga tgttggatag ccagcgccgc cgcgcctctc
     1921 aatactaact gcggctcacc aaatctgaca cgcccgcttt ctcgaccgag aaagcgggcg
     1981 ttgtcttttt cggccctggc aaaccgctat gcctttcggt attctcagtc tcaacatgac
     2041 taccgacatc ggagagcgac gcgctgcttt aagacgagca ttgatcgggc tccttggctg
     2101 cctcactgtg agcctgttcg cagcctgtga aacacccaat ccgcgccaga accttccgcc
     2161 ttctgaaagc gacttagtgc tcctcaagcc ggggaccttg tgccagcgca agacggattt
     2221 ccttcaacag catccgcagt ccagcttgac gatcaagcca tggggcactg gtcaggaatt
     2281 gatgctgccc gcgagtcgca gcccgtctca tggggacgaa tcgttttttt ttgacgatga
     2341 cggcacgctg gtcggcgcac tgttcacatt tgcgtcgggc ctcgacctgg atccctattc
     2401 ggtcctccgc cacaccttga cgctgttgaa acctacgctt gaattttatc tgagcgtcgc
     2461 caacttcgca ggcaagtccg ccatggactc cagctcgttg tacgaaacag gcg
//
LOCUS       DASTQY010000100         4187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_542109, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000100 DASTQY010000000
VERSION     DASTQY010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542109"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VFR82_03185"
     CDS             <1..524
                     /locus_tag="VFR82_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU4500630.1"
                     /translation="ELLTDQPVIICNGFPSREAQKLADRPTHFYMIGSMGSAAAIALG
                     VALAKPHKQVITFDGDGNVLMGMGTLATVAALKPKNFIHVVFDNEVYGTTGNQPTISN
                     VVPLEKVAKAAGYMNVERVLDLDDLVYEFKDMLKKDGPSMLLIKVNEFAEDAGRVLLD
                     PSDITKRFMKAIE"
     gene            637..1725
                     /locus_tag="VFR82_03190"
     CDS             637..1725
                     /locus_tag="VFR82_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEU4500631.1"
                     /translation="MMLLNPGPVNVSERVRQALTRPDICHRESDFAELLQGIQAKLLK
                     AFVPGAEAEYAAILITGSGTAAVESALMSSIPHGKRALIINNGVYGERMSQMVGLHRL
                     GVSELKHEWTERPDPERLRLALRQHPEVHAVGMVHHETTTGLLNPGKAIAEVVDGLNR
                     VFILDAVSALAGESIDIAGSKIYMVAGTAGKCIQGFPGVSFVLVRKGFLERMRAYPKR
                     SLYLHLTHYVDDQGRATVPFTPAVQVYYAFDEALNELLEEGVAKRIQRYKKMAQLIRE
                     RMAKIGIKSVLQPDRQSNSITAYFLPEGVAYPTLHDRLRAMGYVIYAGQGNLENKIFR
                     IANMGALTEAQFIAFLDAFEQIVGGRHT"
     gene            1875..2594
                     /locus_tag="VFR82_03195"
     CDS             1875..2594
                     /locus_tag="VFR82_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEU4500632.1"
                     /translation="MKAVILAAGVGKRLWPVTQHRPKCLIEIGGQTLLSRYLSSLASV
                     GIRRADIVVGYKQEMIRAAADSQSFGVRINFLVNEQFHRGSISSLWVARTALDDDVVI
                     MDADVLFHREILSRLVHSPYPNALLMDDSVKQTGEECMVVAEGGRVIALTKKLPLRYD
                     HTGEGVGFLKVRHMDTPHVVASLRTFVDREAWQMEYEDALIEFFREVKVGYEVIGGLP
                     WTEIDFPIDVEKAERDVLPKL"
     gene            2653..4038
                     /locus_tag="VFR82_03200"
     CDS             2653..4038
                     /locus_tag="VFR82_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU4500633.1"
                     /translation="MTERALDKGTELHGLSTAVLMPSIDLLGAAVRKAVDPLTPVVGV
                     GLFQRTVLTLQRAGIRQLIVVSGQEEDRLKQALLKGPRVSIPVRWMPIREFPLEDPRT
                     WESLAAEVRGFCLFSGVATVFSRSLIEQLRRDVQEGLAIVVTQYGDRPDAQKRWLREV
                     SSGTSEGLADVLVVPTTELSAAAQVAGELETVPISRWLQRAMVDGRVRVTSIDADPSC
                     WYQTVVTSEDLQRAEHRLYTSLKSEFEGYVDRYFNRKLSRWCTRVFIAMGWSPNVITL
                     TATVIGLLSAAGFAVGTYTAGVAAALLFQLAAVIDCSDGEVARLTFTDSPFGAWLDMT
                     LDNVVHMAIFGGIAIGVYSKASGSEYAWLGLALGAAAILGNALSFALVNKAQKITTGS
                     EWNSPRHAALSEFMLKNVASRDFSVIVLLFAVFGKLEWFLWIAAIGSMAFATVMLWIV
                     RPSGIFSRPVR"
BASE COUNT          888 a         1117 c         1224 g          958 t
ORIGIN      
        1 tcgaactatt gaccgaccaa ccggtgatca tctgcaacgg tttcccttcc cgcgaggctc
       61 agaaacttgc cgaccggccg acccattttt acatgatcgg ttccatgggg agcgctgcgg
      121 ctatcgcctt gggcgtcgca ctggccaagc cgcacaagca ggtcatcacg ttcgacgggg
      181 acgggaatgt cttgatgggc atgggcacgt tggcgacggt cgccgccttg aagccgaaaa
      241 acttcatcca cgtggtgttt gacaatgaag tgtacggtac gacaggcaat caaccgacga
      301 tctctaacgt ggtgccattg gagaaagtcg ccaaggcggc aggctacatg aatgtcgagc
      361 gtgtactgga tcttgatgat ctggtctatg agttcaagga tatgctcaag aaggacggac
      421 cgagtatgct attgatcaag gtcaacgagt tcgcagaaga tgcaggacgc gtcctgctgg
      481 acccttccga tatcacaaaa cgatttatga aggcgataga ataagacgct aaccaatgga
      541 cttgcctatg tctgtacttt taacagcccc tattgagtgc tcgtcatcct gttcctccac
      601 acctcgatcg tcacggctcc gtcctgaggt cgcctcatga tgctcttgaa tcccgggcca
      661 gtgaacgtct ctgaacgtgt ccgtcaggcg ctgactcgtc cggatatctg ccatcgggag
      721 tccgacttcg ccgaactact gcaaggcatc caagcgaagc tactcaaggc tttcgtgcct
      781 ggcgcggagg cggagtatgc cgccatcctg atcaccggtt ccggcacagc ggcagtcgaa
      841 tcggccttga tgtcctccat tccgcacggt aagcgcgcct tgatcatcaa caatggcgtc
      901 tacggcgaac ggatgtcgca gatggtgggc ctgcaccggc taggagtgtc tgaactcaaa
      961 catgaatgga ccgagcgacc ggatcctgag cgcttgcgtc tcgcactccg gcagcatccg
     1021 gaagtgcatg cggttggaat ggtccatcat gagaccacga ccggtttact caatcccggc
     1081 aaggctatcg cagaagtcgt cgatggtctc aaccgcgtgt tcattctgga tgctgtaagc
     1141 gcgttggcgg gagaaagtat cgacattgcg ggttcaaaga tttatatggt ggccggtacg
     1201 gcaggcaaat gcattcaagg atttcccggt gtgtcatttg tccttgtgcg taaaggcttt
     1261 ttggagcgga tgcgagcgta tcctaaacgg tcgttgtatc tccatctcac gcactatgtc
     1321 gacgaccagg gccgcgcgac agtgcccttc acgccggctg tccaggtcta ttacgctttc
     1381 gacgaggcgc tcaacgaact gttggaggaa ggcgttgcga agcgcatcca acgctacaag
     1441 aagatggccc agttgatccg cgagcggatg gcaaagatcg gcatcaaatc ggtgcttcag
     1501 ccggatcggc aatcaaacag catcaccgcc tactttctgc ccgaaggggt ggcataccca
     1561 acactacacg atcggctcag ggccatgggc tatgtcattt acgcgggcca aggcaacctc
     1621 gagaacaaaa tttttcgcat tgccaatatg ggcgccttga ccgaagcaca atttatcgcg
     1681 tttctcgatg ccttcgaaca gattgtcggc gggcggcata catagataac aagggagaca
     1741 ggtccgcgga agcccgcaag cccgtgtccc agcctgcagg taagcggact tgttcaatct
     1801 gtacccagct tgttcagcga cgccaaccta tagggctcaa tgtttcactt catgcttgag
     1861 gcaacccgct acacatgaaa gccgtcattc tcgccgccgg agtgggcaag cgcctgtggc
     1921 cggtgacaca gcatcgtcca aaatgtttaa tcgagattgg cggccagacc ctgctgtccc
     1981 gctatttgtc ctctctcgcc agcgtgggaa tccggcgtgc cgacattgtt gtgggctata
     2041 agcaggagat gatccgcgcg gccgcggatt cccaatcctt cggcgtgcgc ataaacttcc
     2101 tagtgaacga gcagttccac cgaggcagca tttcctcact ttgggtggca cgtacggcac
     2161 tcgatgacga cgtcgtgatt atggatgccg atgtgctgtt ccatcgggag attctcagtc
     2221 ggctggtaca ctcgccttac cctaacgcgt tgctgatgga tgattcggtc aagcaaacgg
     2281 gcgaagagtg catggttgtc gctgaaggag gccgcgtgat cgccctcacg aaaaagttgc
     2341 cgctgaggta tgatcatacg ggcgagggtg tgggttttct caaagtgcgt cacatggaca
     2401 cgcctcatgt ggtggcctcg ttgagaacat tcgtggaccg tgaggcatgg cagatggaat
     2461 acgaagacgc tctcatcgaa ttttttcgcg aagtgaaggt cggctatgaa gtcattggag
     2521 ggcttccctg gaccgaaatc gacttcccta tagacgtgga gaaggcagag cgggacgtat
     2581 tacccaagtt gtagcggcgt ctctcccgaa ttcaaaagag gcggaatctg tttctctcca
     2641 acgagtgtat ccatgactga gagagcattg gacaaaggaa cggaacttca cggactctcg
     2701 actgccgtgt tgatgccgtc gattgatttg cttggagcag cggtgcggaa ggctgtggac
     2761 ccgctcacgc ccgtcgtcgg tgtcggtctg tttcaacgta ccgtccttac gcttcagcgc
     2821 gcgggaatca ggcagttgat cgtggtttcc ggtcaggaag aagaccggtt gaagcaagcc
     2881 ctcctcaagg gcccccgcgt aagcattcca gttcgttgga tgccgattcg cgaatttccg
     2941 ttggaagatc cccgcacgtg ggaatcgctt gcggcggaag tgcgtggctt ttgtctcttc
     3001 tcaggtgtcg ccacggtctt ttcacgttcg ctaattgaac aattacgccg ggatgtgcag
     3061 gaaggactgg ctatcgtcgt gacacagtac ggggatcgac cggatgctca aaagagatgg
     3121 ctacgcgagg tatcgtccgg cacaagtgag gggcttgcag acgttctagt ggtgccgaca
     3181 acggaattga gtgcagcggc acaagtggca ggcgaattgg aaacggttcc catcagtcgc
     3241 tggttgcaac gtgccatggt cgatggccgc gtacgggtga cgtcaatcga cgccgatcct
     3301 tcctgctggt atcaaacggt tgtcacgtcg gaagatcttc agcgggcaga gcatcgattg
     3361 tacacctcct tgaaaagtga gttcgaagga tacgttgacc gttacttcaa tcgcaaattg
     3421 tcacgttggt gtacgcgcgt gtttatcgca atgggatggt cccctaatgt gatcacgtta
     3481 acggccaccg ttatcggact tctgtcggcc gccggatttg cagtgggcac ctacaccgcc
     3541 ggtgtcgccg ccgcactctt gttccaattg gcggcagtca tcgactgcag cgacggcgaa
     3601 gttgcccgcc ttacatttac cgattctcca ttcggagcat ggttggatat gacgttagac
     3661 aacgtcgtgc acatggcgat cttcggcggc atcgcaatcg gggtctactc gaaagcgtcc
     3721 ggcagcgaat atgcctggct tgggttggcg ctaggtgccg cggccatcct cggcaacgcc
     3781 ctctcctttg cgttggtgaa caaggcgcaa aagatcacaa cgggaagcga atggaattcc
     3841 ccgaggcatg cggccttgtc cgaattcatg ctcaagaatg tggcgagccg cgatttttcc
     3901 gttatcgtgc tgctctttgc ggtgttcgga aaactggaat ggtttctgtg gattgcggcg
     3961 atcggatcga tggcctttgc gacagtcatg ctgtggatcg tgagaccttc tggcatcttc
     4021 tcgagacctg tacggtgatg ctggggggca gcttttattg gtcgccccgt ccgatcaaca
     4081 tgcacgttgc ggcccagccc tctgcacgcg tgcacatcac gcgaggaaag ccgctgtgag
     4141 tgcggaccgt ggtctcgggg tttccaagtt tctggtcacg gcagatg
//
LOCUS       DASTQY010000101         4121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1549445, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000101 DASTQY010000000
VERSION     DASTQY010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549445"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VFR82_03205"
     CDS             complement(<1..553)
                     /locus_tag="VFR82_03205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4500634.1"
                     /translation="MLAPYSTDSLELSIVIPAHNEAGRILPYLLQVCEYFERQERTYE
                     LLVVDDGSTDATASVVEKLGRANPAVRLFRLPRCRGKGAAVRHGMQSAVGSLQLFADA
                     DGATPIEEFARLEKALADDVDMAIGSRALASRLPGFAVQARFHRTLLGLLFNAAVRRS
                     GIRGIADTQCGFKLFRRVIAQDLF"
     gene            738..1520
                     /locus_tag="VFR82_03210"
     CDS             738..1520
                     /locus_tag="VFR82_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500635.1"
                     /translation="MRRIVEVSNLLLVAVLLVACASATPPSRLGDYVSSDHKGSDEAF
                     SQIEQRPLKAGLLLVSDTFEVGAAPNLPDEALVRLGETLKQDITRAIPVTITQVLSAD
                     QIRPQPNGDWTQFADLGRQHGLEYLVVVILSSTEQEYPVTLFLGWTTHAQPGFRRDNW
                     SLLEFALLDLKNNRTLMQAEGRGWATLDRPSAPGINQWYPVVYLRPQDPERRIWPPTY
                     EGAPNTLRVVSFDQAEKRLALKLQNSWIGQLEADAAERRTAS"
     gene            1806..3197
                     /locus_tag="VFR82_03215"
     CDS             1806..3197
                     /locus_tag="VFR82_03215"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEU4500636.1"
                     /translation="MAQGFAEIVKKVTPAVVNIAVTGGSEGGRGRRPLPPGPFGGPPG
                     GGPPDEPGGELPTPPPMPPPGPHGRPDQSAGSGVIMDPNGYIVTNNHVVEGATQITVT
                     LSDRREFPAKIVGTDPKTDLAVVKIDVKDLPSVKWAEYEKLQVGDLVLAIGSPFGLSS
                     TVTLGIISALGRGNVGIADYEDFIQTDAAINPGNSGGALVNMNGDLIGINTAIFSRTG
                     GSEGIGFAIPSSIAVDIVDSLQKTGKVVRGWMGVAIQEITPALAKSFKLPEQRKGVLI
                     SDVNENGPSHAAGVKRGDVVVSFNGKEVQSVSQLRNLVARTVVGKDAQIKILREGKEQ
                     TLSVKVAERPSDEILAKKESAPPKEQGETIKPPDNVLASLRVQALDNAVMSQLNIPSK
                     TAGVVVSSVEPGGPAEAAGIQRGDVIQEVNHEVVKSLDEYQKAAHKIKKEELAVLLLS
                     RQGNNLFVAVNPK"
     gene            3257..>4121
                     /gene="tatC"
                     /locus_tag="VFR82_03220"
     CDS             3257..>4121
                     /gene="tatC"
                     /locus_tag="VFR82_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU4500637.1"
                     /translation="MLNDFNRWLQDSVFKPLEDKKMPVMEHLVELQVRLTRAVIATAV
                     VFVGTFFYADTLVKWLRVPLQNMFVPGSLSWVPTDLPTVPFVFLAPAEALWQNVKVAG
                     LFAIVLATPYILLEVWQFVVPGLHTQERRFVGPFVALSTVAFYAGVGFSFFFVLPFAL
                     NFLVSYGVSAGFIPQLSIAQYVGFALWFLMVFGLIFEVPLAMTLMAKLGWVDAPFLKR
                     YRKWALLGAFIVAAILTPTPDPFNQCLMALPMYIFYEVGIISAGFFNKKKVQKKPDNA
                     LSPVGPKAMAPA"
BASE COUNT          873 a         1146 c         1220 g          882 t
ORIGIN      
        1 caaataagtc ttgtgcgatg acgcggcgga acaacttgaa cccacactgt gtgtctgcga
       61 ttcctcgtat gccgctgcga cgcactgcgg cattgaacag caggccaagc agcgttcgat
      121 ggaaccgcgc ctggacggca aagccgggga gtcgcgaggc cagcgctcga gagcctatcg
      181 ccatgtccac atcatcggcg agggcttttt cgaggcgagc gaactcctct ataggagtcg
      241 ctccgtcagc gtcggcgaac aattgcagcg aaccaactgc cgattgcatt ccgtgtcgga
      301 ccgccgctcc cttgccccga caccttggca gtcgaaacaa ccgcacggcc ggattggcac
      361 gaccgagttt ttcgacgacc gatgctgtcg catcggtgct gccgtcatcc accaccaaca
      421 gctcatatgt ccgttcttgc cgttcaaaat attcgcagac ttggagaagg taagggagaa
      481 tacgaccagc ctcgttgtgg gctggaatca cgatcgacag ttcgagagaa tccgtagagt
      541 aaggagcaag catggaactc ctacggttcc ggaggggaag cgctgcaagt ccatctctca
      601 ggcggtaccg tagccgaggt gggtggaaaa atacaagagg aaacgtggac ttgacagatc
      661 cagcctaggg gagcgaacgt atatcagcat cgggcacgac catacttgca gcgtccatct
      721 aacggggagg gtcgctcatg cgtagaatcg tcgaggtttc caatctcttg ctggttgcgg
      781 ttctcttggt cgcctgtgct tcggccacgc ctccgagcag gcttggcgac tatgtgtcct
      841 ccgaccacaa gggcagcgat gaggcctttt cacagatcga gcagcgaccg ctgaaggccg
      901 gcctcctctt ggtgtctgac acctttgaag tcggcgcggc accgaatctg cccgacgagg
      961 ccctggtccg cctgggcgaa acgttgaagc aggacatcac tcgggcgatt cccgtcacca
     1021 tcacgcaagt tctgtcggca gatcagatac gtccccagcc gaacggcgat tggacgcaat
     1081 ttgccgactt aggaaggcag cacgggcttg agtacttagt cgtcgttatt ctgtccagca
     1141 ccgaacagga atatcccgtg acattgtttc ttggttggac gacccatgct cagccaggct
     1201 tccgccggga caattggtct ctcctggaat ttgcacttct ggatctcaag aacaacagga
     1261 cgctgatgca ggctgagggg cgaggctggg ccacgttgga tcgaccgtcg gcccccggaa
     1321 tcaatcaatg gtatcccgtc gtctatctgc ggcctcaaga tcctgagcgc cggatttggc
     1381 cccccaccta cgaaggtgcg ccgaacaccc tgcgtgtagt gtcattcgat caggcagaga
     1441 agcggttggc actgaaactg caaaattcct ggattggtca gttagaggcc gatgcggccg
     1501 agcggagaac cgcctcataa gtaaagccag gttgctcgtc atcgtcgtcc ctttcgggtg
     1561 tgttccggcc cgaaggggac gattagtctt tgaaggcctt ggaatggata gtgtacaatg
     1621 aactgttcat ccccatatat taacgaaatc gtataacccc gtcggaggac acggtcatgg
     1681 ctacgtggag tgagaacggt cgtgcggtag catttgccag ttgtatgtgc gtcttcgctc
     1741 ttgcttgtac actgagcccc ttgtcaggcg actcgccggc aacggccgcg ggagtgcctc
     1801 cttccatggc gcagggcttt gcggaaattg tgaagaaagt gacgcccgct gtcgtcaaca
     1861 tcgccgtgac aggtgggagc gaaggcggac gaggacgacg accgcttcct cccggcccct
     1921 tcggtggacc ccctggcggt ggcccgcctg atgagccagg tggcgaactt ccgactcctc
     1981 cgccgatgcc gcctcccggt cctcacgggc gtccagatca aagcgccggg tcgggtgtca
     2041 tcatggatcc caatgggtat atcgtcacca acaatcacgt cgtggagggc gccactcaaa
     2101 ttacggtgac gttgagcgac cgtcgggaat ttccggccaa aatcgtcggg acagatccca
     2161 agacggatct ggcggtggtc aagatcgacg tcaaggacct tccttcggtg aaatgggcgg
     2221 aatatgaaaa acttcaggtc ggcgatctgg tgcttgcgat tggcagtccg ttcggactca
     2281 gctcgacggt caccttgggc attatcagtg cgcttggaag agggaacgtc gggatcgcag
     2341 actacgaaga tttcatacaa actgatgcgg cgatcaaccc ggggaattcg ggcggtgcgt
     2401 tggtcaacat gaacggagac ttgatcggca tcaacacagc cattttctca aggaccggag
     2461 gatccgaagg cattggattc gcaatcccca gcagcattgc ggtagatatt gtggacagtc
     2521 tgcagaagac gggcaaagtc gtgcggggct ggatgggtgt ggccatccaa gaaatcacgc
     2581 cggcgctggc gaagtctttc aagctgccgg agcagcgcaa aggcgtcttg atcagcgacg
     2641 tcaacgagaa tggaccttct catgccgctg gagtcaaacg tggcgatgtc gtggtctcat
     2701 tcaatggcaa ggaagttcaa agcgtcagtc agttgcgcaa tctggtcgcg cgcaccgttg
     2761 tcggcaagga tgcccagatc aagattttac gggaagggaa ggagcagacc ctctcagtca
     2821 aagtagccga acggccatcg gacgagatac tggcgaagaa ggaatcggca cctccgaagg
     2881 aacagggcga gacgatcaag ccgccagata acgtgctggc ttcgctcagg gtgcaggcgt
     2941 tggacaatgc cgtcatgagt cagttgaaca ttccgtccaa gacggccggc gtcgtcgtgt
     3001 cttctgtcga acccggaggc cctgctgagg ccgctggaat ccagcggggc gatgtcattc
     3061 aggaagtgaa ccacgaagtg gtcaaaagcc tcgacgaata tcaaaaagca gcgcacaaaa
     3121 tcaaaaaaga ggagctggcg gttctgctgc tcagccggca aggcaacaac ctgttcgtgg
     3181 cggtcaatcc gaagtaatcc tgaggcgtcg gcgcgccggt tcctcgaccg gcgcctgcgg
     3241 ctctgctggg gatgcagtgc tgaacgactt caaccggtgg ctgcaagatt ccgtcttcaa
     3301 gccgctggaa gacaagaaaa tgccggtcat ggagcatttg gtcgagctcc aggtccggct
     3361 cacgcgggcg gtcatcgcca ccgcagttgt ctttgttggt acgttctttt acgctgacac
     3421 cctcgtcaag tggctccgcg ttccgcttca gaacatgttc gtgccaggct ccctttcatg
     3481 ggtgccgacc gatctgccga ccgtgccgtt cgtcttcctc gctccggcgg aagctctttg
     3541 gcagaacgta aaagtagccg gcctctttgc cattgtcctc gccacgccgt acatcctttt
     3601 ggaagtctgg caattcgtcg taccgggatt gcacacgcag gagcgccgtt tcgtagggcc
     3661 gttcgtggcg ttgagcacag tggcgtttta tgccggcgtg gggttttcat ttttcttcgt
     3721 gctgccgttt gcgttgaact tcctcgtgtc ctatggggtc agcgcgggat tcattccgca
     3781 actctcgatc gcgcaatatg tcggattcgc attgtggttt ctgatggtgt tcggcttgat
     3841 tttcgaagtc ccgttggcca tgaccctcat ggccaaactg ggatgggttg atgcgccctt
     3901 tctcaaacgc taccgcaagt gggcgcttct gggcgccttc attgtcgccg ccattttaac
     3961 ccccacaccg gatccattta accaatgtct gatggccctc cccatgtaca ttttttatga
     4021 ggtgggcatt atcagtgcgg ggttcttcaa caagaaaaag gttcagaaaa aaccggacaa
     4081 cgctctgtca ccggtcgggc ctaaagccat ggcacctgcc a
//
LOCUS       DASTQY010000102         4608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1749955, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000102 DASTQY010000000
VERSION     DASTQY010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749955"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFR82_03225"
     CDS             <1..185
                     /locus_tag="VFR82_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500638.1"
                     /translation="RIPGVEGIKTGFTSKAGRCLIAKVSQNGSDLLLVILNSSRRWNT
                     ATSLITYGLRASGPSL"
     gene            complement(275..808)
                     /locus_tag="VFR82_03230"
     CDS             complement(275..808)
                     /locus_tag="VFR82_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500639.1"
                     /translation="MKAMVRMGVRTCLMMGMIGLVVASALAEPYELSKNDVMDPKAIK
                     SPDISLFGVKIGDTESKAMDVLVNEKIPGIKVEQEALFIFLVDQRKPTGPMAGVRVQD
                     GKVDMIFINNRFAYKTRGLFRNVLNSESPEDIRKLLGNEDYGDENVMGAVLAYDKQGF
                     VINYLGKDVNIEFSPVH"
     gene            complement(980..1273)
                     /locus_tag="VFR82_03235"
     CDS             complement(980..1273)
                     /locus_tag="VFR82_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500640.1"
                     /translation="MDRDTIGRLWEAHKQEGWPRVASQVEGPLMTLDTVISGCVVFFL
                     DSEDGLDEQRRNIVADCLADLEAMEQDLDQEGRAYFRRLRELGALLLIAGRSS"
     gene            complement(1325..2590)
                     /locus_tag="VFR82_03240"
     CDS             complement(1325..2590)
                     /locus_tag="VFR82_03240"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU4500641.1"
                     /translation="MSSLHELDTPTFRLAVAQFDEAAELMGLDSNLRERLKFPQRSLV
                     VSVPIRMDDNRVEVYTGYRVQHDSSRGPSKGGIRYHPDVSLGEVAALAMWMTWKCALA
                     DLPYGGAKGGVAVAPKQLSRGELQRLTRRYAAEIFPLIGPEKDVPAPDVGTDAQVMAW
                     IMDTYSQQVGYAVPGVVTGKPLSIGGSLGREEATGRGVVCVILEALKHLRIAIRHTSV
                     AIQGFGNVGSHTARIMHESGAKVVAVSDVEGGIYNAGGLDIPTLLAKYKGTGQALADT
                     KLGDRITNEELLQLNSTVLVPAALSEQITDRNAAKLNCRVLAEGANGPTTIGADRILQ
                     DKGIFVIPDILANSGGVIVSYFEWVQDAQRFFWKATDIQERLNDLMTSAFQRTLQFAT
                     IRRATMRMAALMIGIDRVAKAHLHRGLYP"
     gene            complement(2596..3384)
                     /gene="lipB"
                     /locus_tag="VFR82_03245"
     CDS             complement(2596..3384)
                     /gene="lipB"
                     /locus_tag="VFR82_03245"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEU4500642.1"
                     /translation="MTAIGFPAQAQVLDPSFRTSFPQGELITIGKPIDYGHAWRLQQR
                     LHSDRVSGRRVDTLILLQHLPVYTVGRRTKPGHVRQDHSSSHDNVPIESVTRGGSVTY
                     HGPGQLVAYPILALSRYAPGPKTYVRMLEEVLLRTLARSNVNGYRVTRAPGVWTGSSC
                     GEVKIASIGARVELGVTLHGFALNVDLNLSPFFRIVPCGLDGCRMTSMAEIARAPVSL
                     ELVGRHMAESFSSVFQLEWRRPYPGTMMPSWVEEEPTISSSGED"
     gene            complement(3381..4418)
                     /locus_tag="VFR82_03250"
     CDS             complement(3381..4418)
                     /locus_tag="VFR82_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU4500643.1"
                     /translation="MATHPLLLIPLVSLLFGYSGASANPGSNTVLSADKTLSVPSVVA
                     KVRSSVVTILTRGVPASPSQIQSGSGSGSGVIIDEGGYILTNNHLVTGVKSMVVGLST
                     GRLTPGRVVARDFLLDLALVKINAQDLVPAVLNPRPDLEIGESVVAIGNPLALKGGST
                     VTVGVVSALDRSVLTPDGETLYDLIQTDAAINPGNSGGPLVDLAGRVVGINVIIAPSA
                     QAISYALSMEAIYPHIQSMMVRGSIYRPDLGFTPVTITPSVMASFGLDGDRGVLALQV
                     DAAKPAGVGGLQNGDIITAVDQHQVFNMGDFWHALLRSGDQSTVQLTLHGRSGPATIQ
                     LPKPAALKAAQ"
BASE COUNT         1016 a         1305 c         1252 g         1035 t
ORIGIN      
        1 gaagaattcc tggtgtggaa gggatcaaga ctggttttac atccaaggca gggcgttgtc
       61 tcattgcgaa ggtgtcacaa aacggcagtg atctgttgtt ggtgattttg aattccagcc
      121 gccggtggaa taccgccacc agcctcatta cctacggact gcgcgcgtca ggcccttccc
      181 tctagcgggt tcgcccgtct aaaataaatc gccagatgaa gagaatcgca agggcaggaa
      241 tgtgaagcgg cttggtgccg cagcatgcat ccggtcagtg aacaggtgaa aattcgatgt
      301 tgacgtcttt gccgagataa ttgatcacaa atccctgctt gtcgtacgcc aacacagccc
      361 ccatgacgtt ttcatcccca taatcttcgt tgcctaatag tttccgaata tcttctgggc
      421 tttcgctgtt cagcacgttg cgaaagaggc cgcgtgtctt ataggcaaaa cggttgttga
      481 tgaaaatcat atccactttg ccgtcttgca cgcgcacgcc tgccatagga ccggtgggct
      541 tcctttggtc gaccagaaag atgaacaacg cttcttgttc gaccttgatg ccaggaattt
      601 tttcattgac cagtacgtcc attgccttgg attcggtatc accgatttta acgccgaaga
      661 gagagatatc cggactcttg atcgccttcg gatccatgac atcgttttta ctcagctcgt
      721 agggttccgc gagcgccgat gctacgacca gaccgatcat gcccatcatg aggcatgttc
      781 ggacacccat tcgtaccatc gctttcatca gcccctcctt ttcccctgat ccaaactccg
      841 gagtggagat ttgatctcaa acagcgctaa attcctcatt tcattctatc ggaggccaca
      901 gcaagttgca ataaatcctg ggccgagtcc ggctctccgg ccacctcggc atgaccgagc
      961 acaaggaacg atatcaacgt taagaactgc gccctgcgat caggagcaaa gccccgagct
     1021 cacggagacg ccgaaaatac gcccggcctt cttgatcaag gtcctgttcc atggcttcaa
     1081 gatcggcaag acagtcggcg acgatgttac gacgctgctc gtccaggccg tcttcgctgt
     1141 ccagaaaaaa cacgacgcag ccgctgatca ccgtatccaa cgtcatcagt ggtccctcga
     1201 cttggctggc cacccgaggc caaccttcct gtttgtgagc ttcccataac cttccgatcg
     1261 tgtctcggtc cactgaaccg ctcctcaggt gggtgcctgt tattgatcca ctgacatgca
     1321 gttgtcacgg ataaaggccg cgatgcaaat gagccttggc aactctatcg atcccgatca
     1381 tcagcgcagc catccgcatg gtggctcgcc taatcgtagc gaactgtagg gtgcgttgga
     1441 aggcggaggt catgagatcg ttgaggcgtt cttggatgtc tgtcgctttc caaaaaaacc
     1501 gctgggcatc ctgcacccat tcgaaatagg aaacgatgac tccccctgag tttgccagga
     1561 tatctgggat gacaaaaatt cctttgtctt gcaaaatgcg gtcagcccca attgtcgtcg
     1621 gaccattggc accttctgcc aatacgcggc agttcagttt ggcggcattg cggtccgtga
     1681 tttgctctga taaagcggcg ggaacgagca ccgtgctatt gagttggaga agctcctcgt
     1741 tcgtgatccg atcccctaac ttggtatccg ccagcgcctg tccggtgcct ttgtattttg
     1801 cgaggagtgt gggaatatcg agcccgcctg cattgtagat ccctccctcc acatcgctta
     1861 ccgccaccac cttcgcgccg gattcgtgca tgatgcgggc ggtgtgcgat ccgacattgc
     1921 cgaagccttg aatggcgacc gaggtatgac gaatcgcgat tcggaggtgt ttgagcgctt
     1981 ccaggatcac gcagaccacc ccacggccgg tggcttcttc tcgaccgaga cttcctccta
     2041 tggaaagggg cttccccgtt accactcccg gcacagcata accgacctgc tggctgtaag
     2101 tatccatgat ccacgccatg acctgggcat ctgttcccac atccggagct gggacgtcct
     2161 tttccggtcc gatcagaggg aaaatctccg ccgcataccg gcgggtcagg cgttggagct
     2221 cgcctcggga cagttgtttg ggtgcaacgg ccacgccgcc cttggcgccg ccgtatggca
     2281 gatcggccag ggcgcatttc caggtcatcc acatggccag tgcggccacc tcacccagac
     2341 tgacgtcagg gtgatagcga atgccgcctt ttgacggccc tcgagacgaa tcatgttgga
     2401 cacggtatcc ggtatagacc tctacacgat tgtcatccat ccgtataggc acgctgacga
     2461 ccagcgatcg ttgtggaaat tttagtcgtt cacgcagatt cgaatccaag cccatcaatt
     2521 ctgcagcttc atcgaattga gcgactgcca gtctgaatgt cggcgtgtcg agttcgtgca
     2581 gggaagacat cagcgctagt cctctcccga cgatgaaatc gtcggctcct cctcaaccca
     2641 ggaaggcatc atagttcccg gatatgggcg cctccactcg agttgaaaca ccgatgagaa
     2701 gctttccgcc atgtgccggc cgactagttc aagcgatact ggcgctcgcg cgatctctgc
     2761 catcgaggtc atgcggcatc cgtcaagacc acagggcacg attcggaaaa acggcgagag
     2821 attaagatcc acgttcagtg cgaacccgtg cagcgtgact ccaagttcaa cacgtgcgcc
     2881 gatcgaggcg atcttgactt caccacatga cgatcctgtc caaacacctg gcgccctggt
     2941 cacacgatag ccattcacat tcgaacgagc gagcgtcctc aggaggactt cttcaagcat
     3001 gcgcacataa gtctttggtc ctggcgcata acgagagagt gccagaattg gataggcgac
     3061 taactgtccg ggtccatgat aggtcacgga cccgccgcga gtgacggatt ctattggcac
     3121 gttgtcatgg ctggaggaat gatcctgtct cacatgtccc ggtttcgtgc gacggccgac
     3181 ggtataaacc ggcaggtgct gaaggagtat gagcgtgtcc actcgtcttc ccgacacacg
     3241 gtccgagtgg agtcgttgct gtaggcgcca ggcatgtcca taatcaatgg gcttgccaat
     3301 ggtaatcagc tctccttggg gaaatgacgt cctgaaagag ggatcgagca cttgtgcctg
     3361 tgcaggaaaa ccaatagccg tcactgtgcg gccttcaacg cagctggttt tgggagctgg
     3421 attgtggcgg gaccgcttct gccgtgcaaa gtgagctgga cggtagattg atcccctgaa
     3481 cgaagcagcg catgccaaaa gtcccccatg ttgaagacct gatgctgatc gaccgccgtg
     3541 atgatgtcgc cgttttgcaa accgccgact cctgcaggtt ttgctgcatc gacctgtaag
     3601 gcaaggacgc ctcgatctcc gtccagacca aaactggcca tgacgcttgg ggtaatggtc
     3661 accggcgtaa accccaaatc cggtcgatag attgacccac gaaccatcat ggattgaatg
     3721 tgagggtaaa tcgcctccat cgacagggca taactgatgg cttgcgccga tggcgcgatg
     3781 atgacgttga tgcccaccac tcggccggct aagtccacaa gggggccgcc gctattgcct
     3841 gggttgatag ccgcatcggt ttgaatcaag tcgtagagcg tttcaccatc aggagtcaag
     3901 accgatcggt ccagcgcact gacgacgcct acggtcacgg tagagccacc cttcagcgcc
     3961 agggggttgc cgatggcgac tacggactca ccgatctcca gatcgggtct cggattcaac
     4021 accgccggaa cgagatcctg tgcattgatt ttgacgagag caagatcgag cagaaaatct
     4081 cgagcgacga cgcgccctgg cgtcagacgg ccggtcgaaa gtcctaccac catgcttttg
     4141 acgccggtta caagatgatt attggtcaga atgtagcccc cttcgtcgat gatgacaccc
     4201 gaccccgaac cagaacccga ctggatctgt gacgggctgg ctggcactcc acgtgtcagg
     4261 atggtgacca cagaggaccg gactttggcg acgacggatg ggacggagag cgttttgtcg
     4321 gccgagagga cggtgtttga accggggttg gccgaggcgc cggaatagcc gaaaagcaga
     4381 ctcaccagcg ggatcaagag tagaggatgg gtagccatag aattcacacg tgaactgcgc
     4441 tcattgtctc acaactatcg attccggaaa aggcgcaata aaaaaggggt gaggagttgt
     4501 ctcctcaccc ctttgtgcgt ccccgatgtc gccattattg gatggcggca aacatttcct
     4561 ttatcgccgg tgtcttgagc ttcttgagcg cttttgcctc gatttgtc
//
LOCUS       DASTQY010000103        15634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1639969, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000103 DASTQY010000000
VERSION     DASTQY010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 15634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15634
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639969"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1197..4340)
                     /locus_tag="VFR82_03255"
     CDS             complement(1197..4340)
                     /locus_tag="VFR82_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4500644.1"
                     /translation="MNQLSVLIVEDEAILAAHLASKVKMLGYRVIGPVSSGEEAIELA
                     QIEGPDLALLDIRLEGQLDGIQTAKLLKRWREIPVIFLTAHSDQETIKKASSIGPFGY
                     ILKPFEERDLATQLEITFYKHQAEASLQKSEERYRAFIQNSSEGIWRMEFDPAVDVAL
                     PIEEQVKRMYDSARLVECNDAMGRMYGFSNAEGLIGKTLDFMLPASDPSAREFLASIV
                     RAGYRATDMESMERNVFGETVYFLNNMVGIVHDGFLVRMWGTQQNITDRKCAEEAIRE
                     SEARTRIAQQAARWGVFEYNYKTGKNYWSVELEALYGLRPGSFEGTYEAWLGRVHPND
                     CSEAERAMDRALFTNEYTHDFRVIWDDGSVHWLFARAKIFRDANGAPERMLGVNVDIT
                     ERKQAEEALRHSEEQLELISDTVPALISYVNRERRYVTCNEAYTKWFGLSRNDIVGKT
                     MQEVVGAEAWGAMAAYVEAALQGQTVDYEAEARYRYGGTKWIHAVYTPHRDAEGHIAG
                     FIVMVTDISARKRTEAALRESEMRFRTIADSAPVLIWMNGMDGCEFVNHAYLEFIGSR
                     QQEDVVNYEWAQYIHDDDRENYLQLYFDAMRDRLPFDSVCRFRRFDGQYRWMRSVGRP
                     RLASNDKLIGYVGANVDITDIQEAQDRLQRWSDELTKAVSVKTHELLESQDRLRSLAA
                     EVGRAEQRERKRIATELHDHLQQMLVFCKLRLGQGKQQAAAIPACADVMKQVDDVLSE
                     ALTYSRTLVAQLCPPVLQEFGLAAALQWLGDQMSQRELHVSVHVDHIPVPEEQAVLLF
                     QSVRELLMNVVKHAGTTHASVEMKRDNRALRIEVRDSGSGFDPAVVQPYASAQPPKFG
                     LFSIRERMKAFGGMLEVETALGQGTSAVLLLPLEDTKNETSESRLYDQPVPVGAMKPG
                     AREKPRPSEKQRLTVLLVDDHAMMREGLRSVLEAHSDIHIVGEASDGQEAIALVDELR
                     PSLVVMDINMPRLNGIEATARIKARYAEIKVIGLSVNAGPNNKEAMLRAGADMLLTKE
                     AAVEELYRGIREVMPRL"
     gene            4531..4731
                     /locus_tag="VFR82_03260"
     CDS             4531..4731
                     /locus_tag="VFR82_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500645.1"
                     /translation="MNYIRIGKKALNLDHVTYAEVQVWQDEMSVKVYFSGSANNAPVV
                     FAENEAKELWKYLEYVAEKPVG"
     gene            complement(4772..5422)
                     /locus_tag="VFR82_03265"
     CDS             complement(4772..5422)
                     /locus_tag="VFR82_03265"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU4500646.1"
                     /translation="MNKNEKQTRSSQLISLWLIIAGTVGSGITPSTVAESGFYGQVPI
                     LYEEVKHSDISNLPAITPGNSMASGTELQHAVIRAARNHRLHPALLYAVMKAESSFNP
                     IVVSKAGAVGLMQLVPETAMRHGVRNLYDTNENVAGGARHLRYLLDRFHGNTRLALAA
                     YNAGEGKVDRYKQIPPYRETRHYVQKVLTYYREYRKEVPIAAPVEGHSGGYAMRAL"
     gene            5673..6011
                     /locus_tag="VFR82_03270"
     CDS             5673..6011
                     /locus_tag="VFR82_03270"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4500647.1"
                     /translation="MKIRYSERVLTDFPVIFAGDAYVGEGVVSNVSVPGCEILSNKPV
                     EPGTYLEMKLMMPDSGPSLSVGLAKIRWCRGRRFGVEFIRMPGGDQVRLGRLVKQQRV
                     LRPTARLHHS"
     gene            6049..6372
                     /locus_tag="VFR82_03275"
     CDS             6049..6372
                     /locus_tag="VFR82_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500648.1"
                     /translation="MTGRLLLLLSCILFSGCAEPFTVFHSQTGEQILLGRRSFSYDDC
                     LSSIREEANRLGMTLRYVHVRGSVIGRSLLWPFEPGYACEAAAGPEGHPNGIYYNVPP
                     RVPQG"
     gene            6652..7029
                     /locus_tag="VFR82_03280"
     CDS             6652..7029
                     /locus_tag="VFR82_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500649.1"
                     /translation="MEDLVIEEEGASSALKITLGDFLVFINWMNQTEKALERGEALPT
                     TPDLDSGKVKGWIQLVQRLEERFRRSEEKKIALEANVIAQEAQLGQLLTHLHANIASL
                     YDNLGQAKKAEEVRRRAEALHAT"
     gene            7256..8203
                     /locus_tag="VFR82_03285"
     CDS             7256..8203
                     /locus_tag="VFR82_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HEU4500650.1"
                     /translation="MISVLLIEDNDVDARLTQDLLAEWSVEEFKVTHAKTLSDGLAYI
                     DKGRFDAILLDLSLPDAFGLPTVRQVHATNPSIPVVVLSGVSDQSLALQAVQQGAQDY
                     LVKGQGHPELLARSVRYAIERKRSEERLTYLAQYDQLTGLVNRTLFRDRLIQAMARSK
                     RLQQPLGLMLLDLDRFKSVNDTLGHDVGDDLLKAVAERLKGCVREVDTVARMGGDEFT
                     IVLEGVASEQSIVVVAKRITESITSVFPLKGQTVSVGISIGITVYPHDDHSIDELLKH
                     ADSAMYRAKQQGGSGFAFHEASGYNHAATYSTSRPSAFQ"
     gene            complement(8225..9034)
                     /locus_tag="VFR82_03290"
     CDS             complement(8225..9034)
                     /locus_tag="VFR82_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEU4500651.1"
                     /translation="MSVESQPATSSPPPNPLGGKTLIVDWQDPNVYPRPSAALKDAGD
                     QDQVFVRAGIYEDKIFISARPVLLTGAGRDAVQIYSRLGGPLYLQRVPGGRISGLTFR
                     YVGSDQHSAINVLDSSCTITSCRATEGILSGVVIYGPECRPAFIDNEVSCNRESGIFV
                     FAGAQPRVADNVCRGNHHFGIAVRDRGSRPELVRNRCSDNWLSGMLFFHHAEALLVDN
                     VCTDNMHWGVVATPDSRLLPPPDGLAGANVVVPNPRGALHITEEPLSEIGR"
     gene            9291..9800
                     /gene="ybgF"
                     /locus_tag="VFR82_03295"
     CDS             9291..9800
                     /gene="ybgF"
                     /locus_tag="VFR82_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEU4500652.1"
                     /translation="MKTLALVPLGILASLVMIAGHPTKTLAAPSQVQDTSRHLYDRVM
                     EEFKHRDYEAALAGFRLFLELHGQTSLAANAQYWVGECQFRMGRYKEALNAFYNVVSY
                     YPLSQKLAASTLKIGQTYTRLKDHEKARMMYERVVDQYPDSPEAEVARKAIEVEAAKI
                     EPSLPPVNE"
     gene            complement(9814..10341)
                     /locus_tag="VFR82_03300"
     CDS             complement(9814..10341)
                     /locus_tag="VFR82_03300"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HEU4500653.1"
                     /translation="MRQAAIDTPFIALIAAVLVFLLWRIVSGWSGKAHSAGPFTLPLG
                     VVLRPQPLLAEKELLLYNLIRLAVEDRYLVFAQVPLWKFLMVEAAGDARIQVLRHLAL
                     KRADFALVHPGSRVVEQVVQLDEESPTDPDRSTNGREVQRILHSAGIRVTTLKAQPSY
                     TVQELEQLLGVSDLE"
     gene            complement(10338..11528)
                     /locus_tag="VFR82_03305"
     CDS             complement(10338..11528)
                     /locus_tag="VFR82_03305"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEU4500654.1"
                     /translation="MQRKRMKIVGLMSGTSGDGVDAALVEVSGRGLSLRARALAAQTL
                     SYPRSLQQRILAASVSGTVAEICHLNALLGEWFADAALSVIRQAKLSPADIAVIGSHG
                     QTVHHLPQGIHAGGAGAIRSTLQIAEPAVIAERTGITTVANFRPRDMAAGGQGAPLTP
                     AVHALLLRHPQRSRLIVNLGGISNVTYVPRGGEWSGVLAFDTGPANMVLDGLVNRATN
                     GRIAMDRDGKLALKGRADPRLLGKLLAHPFLSRRPPKSTGREAFGPSLIDELVSVQKQ
                     RGLSIEDLLATCSLWTAKAVGTARRWIKGTIDEVVVGGGGVRNRAVMDQLTAVFGAVP
                     VSTFDSLGWDSKAFEAVAFAVLAYLTVTSQSGNIPSVTGAKHPVLLGAIVPAAPGWMK
                     HFRA"
     gene            complement(11544..11756)
                     /locus_tag="VFR82_03310"
     CDS             complement(11544..11756)
                     /locus_tag="VFR82_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500655.1"
                     /translation="MTTTFTGSDDVATATEAEHVSQEVARIAKDHVQKDPENMGGDRS
                     IEVSWMSTRPHGSIGCVRLDQALKFY"
     gene            11746..12567
                     /locus_tag="VFR82_03315"
     CDS             11746..12567
                     /locus_tag="VFR82_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEU4500656.1"
                     /translation="MVVTIPVLKDLAEQAGGPYIRVTSLLKGYENEHTYSPKPSDLIA
                     VRKARLLFEVGLGLEVWVTNLVKNAGSASLRIITTSKGIGIMRDDQEPVEAHHSLAHE
                     QANPHVWMDPENAATMLRHITDALIEVDPDHAHEFRQNQAAYLKRLDLIRDELTERVR
                     KLPDRRFVAHHPAWPYFARRFGFQIAGTIQAQSGSEPSAMHIQHLISTIKKQRIRAVV
                     SEIQLSQKLPELLAKETGIRIVVLTTLPGGIPQTETYLDMLRYNVLQLANALEAA"
     gene            12621..13412
                     /locus_tag="VFR82_03320"
     CDS             12621..13412
                     /locus_tag="VFR82_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU4500657.1"
                     /translation="MSASSDTIIQFDHATFGFPGVIALKDLSLAIQAGEFIGVIGPNG
                     SGKTTLCKAVLGLMAPVEGNLRIFDCACDELRCHHRAKIGYLPQKGVVDRNFPVTVLE
                     TVMMGRYGAMGLLKRPGTTDRLIAMEALTHVGMERHKHTALGHLSGGQQQRVFIARAL
                     AQRPQVLLLDEPTTGLDMTTQHNVIELIEHLHQELRLTVLLITHDINMIRSRVDRLIL
                     LKTRLYAAGPPAEVLKPDILREVYGKDLVITDKDLIIVEDYHHHH"
     gene            13431..14255
                     /locus_tag="VFR82_03325"
     CDS             13431..14255
                     /locus_tag="VFR82_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU4500658.1"
                     /translation="MLELLSYDFMQRSLFAAAMVGALCSVIGVFVVLRGLAFVGAGTA
                     HAAFAGVALAYLIGWPPLVLAILFGLVTVWITGWVEEKGTMKLDVSIGILYTTTMALA
                     ILFIGLMKTYNAEVYGYLFGNVLAVTAEELRTISALSIVVLGIILLFSKELYFIAFDQ
                     EMAEASGVPARMMFFLLLTLVAFTVVVSLKTVGAILVFAMVLIPASTAYQLTHSLSTL
                     TWYSVIIGITCAVGGVLLSAVWDIPSGPAIVLLSTFLFFLSVVLSPKRLRRSISNV"
     gene            14406..15041
                     /locus_tag="VFR82_03330"
     CDS             14406..15041
                     /locus_tag="VFR82_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4500659.1"
                     /translation="MRILWMGLLVTGLWINSAYAVDMKMQDGGIEPGKVVLGMRAGFA
                     PLTQQLTPGTSTDVGSLVNFQAMYSLNNWFLIGMMVEWERHSISTEGGFDIGHQDTVS
                     VLPTVELRPVRFGPISPYVNMSFGVNVNSFGEDRAIAGNRISPSNTFAWRLGWGADWM
                     ITKQFALNGEMAYKRNDGHATVTGPGPVGGGRVDDWNASSFGFLFGVKMFF"
     gene            complement(15114..15545)
                     /locus_tag="VFR82_03335"
     CDS             complement(15114..15545)
                     /locus_tag="VFR82_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4500660.1"
                     /translation="MPAALKTAVTFSEKKELEASQLLRLFHQAPWAKDRTLDDAKDML
                     RHTDLALCAWDGDQLVGFGRVLTDFVYRATIWDVIVDKAYQKQGIGSDIVQRILNHPR
                     LKRVELFWLCTRRPSFYGKLGFSSKEQTGMVWSRSKQARQE"
BASE COUNT         3450 a         4431 c         4174 g         3579 t
ORIGIN      
        1 actgccctta tgggagagat ccgctgctcc gctgctgaag atctcagcga tgcagacttc
       61 gttccttcta ctccctttca gttccggcgg ctagggaaca tcctaggaga cggttcctca
      121 gattaaacat agattgcacg ccgatacgct tgtgcctcgt ggtggggtgt cgagaaatgc
      181 ctgaaggctg atgaggtgct gccacgactc agggagggta ccatgcagca tgccaaagca
      241 ttccacagtt tctctgatgc ctgcgaacgg ttcgtctcat ccatagccac gaacaggtcg
      301 ctgtcgaatg atgaagcaca agtcatagca ttctattgca aggaaattct cgaaaagata
      361 gaaccatgtt tgcataagcg ttcccacgac atttcgagcg gagcttcgga ggtaggccgg
      421 acggcgaata catagttcat catggttgtg cttgtatctg tcgtcttgaa tgtcccggag
      481 agagcaatga ggtcgcgtac aagtgggtct tcgtcatctt ctcatgctgt agtaaaacgg
      541 ctcaacctca tcatgggttg acagccaggt tgaaagcacc ttgaccgatg aaaatatgct
      601 tggtgagcac atgtccatga acaaatgcgg ccggtgtgga cgtccgtgct tgagaaggag
      661 atcatcttga cgagtgagcc atcgggccgg agatgcatca ctggttacca ctgtagctcc
      721 gatggctact cgagcgtaat ccctttactc tcactcagtc tcgaagagct atcgatgctg
      781 taccgcccag atcaattcct tctgcctggt aggcatatac ccttttagcc cggaacgcgt
      841 ccagtaaacc ttacgaggca gacgtcctaa ggtctgcact cttcaccttg cattgtatga
      901 ataggaggtt gaatgccgtc ccccatgact gtgtctctag aagttcccta tgctgagaac
      961 gagcaacaat ttctggctct cgtggttcag accgctgtca agacgtttag gagtttaaat
     1021 gcttctcctg ccgatgagtc agcatcgaaa acttccgtcg ggtctataga agcgccggca
     1081 tggtcttcat caatcaatgt gtcggaagac agggcgaaga gaagctaagc ttgacgccgc
     1141 tttgtgcaag ggctcgatga ccctgaaatg cgccccgaaa agtggtgcgt gggtttttac
     1201 aagcggggca tgacttctcg aatgccgcga taaagctctt ccaccgctgc ttccttggtt
     1261 aagagcatat cggccccggc tcggagcatg gcctccttgt tattcggccc cgcgttcacc
     1321 gacaaaccga tcactttgat ctcggcgtat cgcgccttaa ttcgtgctgt agcctcgatg
     1381 ccgttgagtc gcggcatatt aatatccatc acgacgagag agggtcggag ttcatccacc
     1441 aacgcgatgg cttcttggcc atcagacgct tcaccgacaa tatgaatgtc tgaatgcgcc
     1501 tctaatactg atcgcaagcc ctctcgcatc atcgcatggt cgtcgaccaa aagaaccgtc
     1561 agcctctgct tctcactcgg tcgcggcttc tctcgtgcac ccggcttcat cgcgccgacc
     1621 ggcaccggtt gatcatagag gcgcgattcg gacgtctcgt tcttggtatc ctcaagtgga
     1681 agcaacagca cagcggatgt tccttgtcca agggctgttt ccacctccaa catcccgccg
     1741 aacgccttca tccgctcgcg aatggaaaag agtccgaact taggcggctg agcactggca
     1801 tatggttgaa caacggcagg atcgaatccg ctgccgctgt cgcgcacctc gatgcgtagg
     1861 gctctattat ccctcttcat ctccacactc gcgtgagtgg ttccagcatg tttgaccaca
     1921 ttcatcaaca gctcccgcac cgattggaat aggagcacgg cttgctcttc agggacaggt
     1981 atatgatcca catgtacaga gacgtgcaac tcacgttggc tcatctggtc gcccaaccat
     2041 tgaagagcag cggcgaggcc gaactcctgc agaactgggg gacacagctg ggccaccaac
     2101 gttcgcgaat acgtcagggc ttcactcaag acatcatcaa cctgtttcat cacatcagca
     2161 caggcaggta ttgcggccgc ctgctgcttg ccctgtccta gcctgagctt gcaaaacacc
     2221 agcatctgtt ggagatgatc gtgcaattcg gtggcgatgc gctttcgttc ccgttgttcg
     2281 gcacgaccca cttctgcggc cagcgaccgc agtcgatctt gagattccag cagttcatgt
     2341 gtcttgacgc tcacggcctt cgtgagctcg tcgctccatc gttggagacg atcctgcgcc
     2401 tcctgaatgt cagtgatatc gacattggcg cccacatatc cgatcagttt gtcgttcgac
     2461 gcgaggcgtg gccttccgac cgagcgcatc catcgatatt gcccatcaaa gcgtcgaaaa
     2521 cgacacacgg agtcaaaggg gaggcggtct cgcatggcat caaaatacaa ctgtaagtag
     2581 ttctctcgat cgtcgtcgtg gatgtattgt gcccactcgt agttgactac atcctcctgt
     2641 tggcggctcc cgatgaattc caaataggca tgattgacga actcgcagcc atccattcca
     2701 ttcatccaaa ttaaaactgg cgcgctgtcg gcgatggttc gaaatctcat ctcgctttca
     2761 cgcaaagcgg cttctgtacg ttttctggca ctaatgtcgg tcaccataac gatgaagcca
     2821 gcgatatggc cttcggcgtc tcgatggggc gtatacaccg cgtgaatcca ctttgtacca
     2881 ccgtatcggt aacgggcttc ggcctcgtaa tcgaccgtct ggccttgaag agctgcctcc
     2941 acatacgccg ccattgcgcc ccacgcttct gcccccacga cttcctgcat cgtcttgccc
     3001 acgatgtcat ttcgggacag accaaaccat ttcgtgtagg cctcattaca tgtgacatat
     3061 cggcgctcgc gattcacgta cgagatcagc gcgggcacag tatcactgat gagctccagt
     3121 tgctcctcgc tatggcgtaa cgcctcctcg gcctgtttgc gctcggtgat gtcgacattg
     3181 acccccagca ttctctctgg agccccgttc gcatcgcgga agatttttgc tcgagcaaat
     3241 aaccaatgga cgctaccgtc atcccaaata acgcgaaaat cgtgcgtata ttcattggta
     3301 aagagcgccc ggtccatagc tcgttcagcc tcagaacaat cattgggatg gacacgccct
     3361 agccaggcct cataggtgcc ctcgaatgac cccggccgaa gcccatacag cgcttcgagt
     3421 tcgacagacc agtagttctt tccggtcttg taattgtatt cgaatacccc ccagcgagcc
     3481 gcctgctgcg cgatccgggt acgcgcctcg ctctcacgaa tggcctcttc ggcacatttg
     3541 cgatcggtga tattttgctg cgtgccccac attcgtacga gaaaaccatc gtggacaatg
     3601 ccgaccatat tattaagaaa atacaccgtt tccccaaaca cgttgcgctc catcgattcc
     3661 atgtcggtgg cccgataccc ggcccggaca attgaagcga gaaactcgcg cgccgaaggg
     3721 tctgaggcgg gcagcatgaa gtccagagtc ttcccgatga gcccttctgc attgctaaat
     3781 ccatacatgc gtcccatcgc atcgttgcat tcgacaagac gtgccgaatc gtacatgcgc
     3841 ttcacctgct cctctatcgg aagagcgaca tcgacggccg gatcgaattc cattcgccag
     3901 atgccttccg agctgttctg gataaaggct cgatatcgtt cttcactttt ctgcaacgac
     3961 gcttcggctt gatgtttgta gaacgtgatc tcgagctgag tggccaggtc tcgctcttcg
     4021 aagggcttca agatgtagcc aaatggaccg atcgaactgg cttttttaat ggtctcctgg
     4081 tccgaatgcg cagtcaggaa aatcacaggt atttcccgcc acctctttaa gagtttggcc
     4141 gtttgaattc cgtctaattg gccctctagc cggatgtcga ggagggcaag gtcggggcct
     4201 tctatctgag cgagttcaat ggcttcttct cccgaggaaa ctgggccaat gacacgatag
     4261 ccaagcatct ttactttact ggcgagatga gccgccaaaa tggcctcatc ttcaacaatt
     4321 aggacagaga gttggttcat accgaccaac cagtggatgg ggaaaagcgg ggcaagtata
     4381 agtgggagcc ggtcgtggaa gggcctaggg gaaaccctgg ctagagatat ctataccttt
     4441 cctatgtttg cggtgcagaa aacggctaac tcattgacgg actcccgacc ggtgctatag
     4501 ttcgaaagct aaatacctag aggggaatcc atgaactaca tccgtattgg gaagaaagcc
     4561 ctgaatctgg accatgtcac gtatgctgaa gtccaggtct ggcaggatga gatgagcgtc
     4621 aaagtgtatt tttccggctc ggcgaacaat gcgcctgtgg tttttgcaga aaacgaggcc
     4681 aaagaattat ggaagtattt ggagtacgtt gcagaaaagc ctgttggata gccgacccga
     4741 ccagtcaaat tgtccgactt cccccctcgc gtcacaaggc ccgcattgca taccctccgg
     4801 aatggccttc caccggtgca gcaatcggaa cttctttccg atactcacga tagtaggtca
     4861 gcaccttctg gacataatgc cgagtctccc tatacggcgg aatttgcttg taccgatcga
     4921 cttttccttc tcctgcgttg taggcagcca acgccagacg cgtgttaccg tgaaaccggt
     4981 cgagaagata ccgaagatgc cgtgctcctc cagccacatt ctcattggta tcatagaggt
     5041 ttcgcacgcc atgcctcatg gccgtttctg gtaccaattg cataaggccg accgccccgg
     5101 ctttagagac gacaatcggg ttaaacgaag actcggcttt catgactgca tacagcaaag
     5161 ccggatgtag gcgatggttc cgggctgccc gtatcacggc gtgttgtaat tccgtacctg
     5221 aagccattga attgccggga gttatcgcag gaaggttgga aatgtcgctg tgtttcactt
     5281 cttcgtacaa gatcgggacc tgtccataaa atccgctttc ggccacggtg gaaggtgtga
     5341 tacctgagcc cacagtgcct gcgatgatca accacagaga gatcaactga ctgcttctgg
     5401 tctgcttttc attcttgttc atgtcgtcct ccaagagaca cttcccgctt cctttcaacc
     5461 atctacctga tcccctatcc gcagttttcg tgcccctgat gggcacccta aaatcaatac
     5521 attctccttt aacaataaca acttaggcag ttcatgccct cccaactttt gtagggatgg
     5581 cacggtgcgc tgattccata taatgtctgt cgaacgcaat ttgcgtcttt aacagggcga
     5641 ggccggtcat cggagtatgg ggagggcaag gcatgaaaat ccgctacagc gaacgggtcc
     5701 ttaccgattt ccccgtcatt tttgctggtg atgcctatgt cggcgagggt gtggtcagta
     5761 acgtctcagt gccaggatgt gagatcctaa gcaacaagcc ggtagaacca ggcacctatc
     5821 tcgagatgaa actcatgatg cctgacagcg gaccttcact cagcgtaggt ctggccaaga
     5881 ttcgttggtg caggggacgt cggtttgggg tggaatttat ccgcatgcca ggtggcgatc
     5941 aggtccgcct ggggcgactc gtgaagcagc agcgtgtctt acggccgact gctcgactac
     6001 accactcgtg aatgctacac ttcctcttag gctcttggag ctctcgtcat gaccggtcgt
     6061 cttttactgc tcttatcttg cattctgttc tccggctgcg cggagccgtt taccgtattt
     6121 cacagccaaa cgggcgaaca gattcttctg ggccgtcgtt ccttttctta tgatgactgt
     6181 ctttcatcca tccgtgagga agccaaccgc ctcggaatga ctcttcgtta tgtgcacgtg
     6241 cgaggatcgg tgatcggccg gtctttgctc tggcccttcg aaccaggcta cgcctgtgaa
     6301 gcggccgccg gacctgaggg acaccccaac ggcatttact acaatgtgcc tccccgggtg
     6361 cctcaaggat aaatctcctt ccttaccttc ttcgttcaat acaaattgta aaattcgact
     6421 catcaggagt tgagttgttg acaagcgaac cgcgatcgca gtattactgc acaacgacag
     6481 tatcacgcct gatgaaacac ctgctcgtgc atccgtcaga acctcttcca ctcacctgaa
     6541 aggagccggc ctgcgtggaa ccaggcatca acgcgtttcc gtctgtaatc gtccgtcaca
     6601 gcttggatcg actggcctca ttcgtcgtct catcatctca aaggagtcat catggaagat
     6661 ctcgtgattg aagaagaagg cgcctcctca gcattgaaga tcaccctcgg agacttcctc
     6721 gtgttcatca actggatgaa tcaaaccgag aaggcgcttg agcgaggcga agcactgccc
     6781 accacccctg atctagattc agggaaggtg aaggggtgga ttcaactcgt gcagcgtttg
     6841 gaagagcgtt ttcgccggag cgaagaaaaa aagatcgctc tcgaagccaa cgtcatcgca
     6901 caagaggcac agctcgggca gttgctgacg catttgcatg ccaacattgc ctcgctctac
     6961 gacaatcttg ggcaggctaa gaaggccgaa gaagttcgtc ggcgcgccga ggcgttgcac
     7021 gccacctgac gggggccctc cggggcagcc cgttagaccg tctctcattc accacggttc
     7081 ttttccgacc tgagtgacag gggttggtgt cccttgacgc cctgacatga tgattcccac
     7141 attttacttc tcgcctagca gccgttagct ataatcaaac tcagtagcgg tacatgcctg
     7201 tcaggtcgaa acatcggcct ggaatgtcca agttttagag ccggattttt gcacgatgat
     7261 tagcgttctc ttgattgaag acaatgacgt cgatgctcgc ttgacgcagg acttgctggc
     7321 ggagtggagc gtcgaagagt tcaaagtcac acatgcaaag acgctctccg acggactggc
     7381 atacatcgat aagggccgct tcgatgcaat tctgctcgac ctctcgcttc ccgatgcctt
     7441 tggcctgcca accgtgcgac aagttcatgc caccaatccc tccattcccg tcgtcgtgct
     7501 gagcggggta agcgaccaat cgctcgccct tcaggccgtg cagcaaggag cccaggatta
     7561 tctcgtcaaa ggtcaaggtc atcctgagct gctggctcga tcggtgcgct atgcaatcga
     7621 gcggaaacgc tcggaagagc ggttaacgta tctcgctcaa tacgatcaat tgacggggct
     7681 cgtcaaccgt acgctatttc gcgatcgctt aattcaagcc atggccagga gtaagcgcct
     7741 tcagcaaccg ctgggcctga tgctgttgga tttagaccgc ttcaagtcag tgaatgatac
     7801 gctcggccac gatgtcggtg atgacctgct caaagctgtt gcggagcgat tgaaaggatg
     7861 cgttcgcgag gtcgataccg tcgcacgcat gggtggggat gagtttacca ttgttctgga
     7921 aggcgtcgcg tccgagcaaa gtatcgtggt cgtcgccaag cggattaccg agtccatcac
     7981 gtcggtcttt ccgctcaagg ggcaaacggt ctccgtcgga atcagtatcg ggatcacggt
     8041 atatccccac gacgaccata gcatcgacga gctcctcaaa catgccgact ccgccatgta
     8101 tcgagcgaag cagcaaggtg gcagcggctt tgcgttccat gaggcctcgg ggtacaacca
     8161 cgccgccacc tattcgacat cccgaccctc cgcattccaa taacaccgcc tagcactagc
     8221 cctgtcagcg tccgatttcc gaaagcggct cctcagtgat gtgcagagcc ccgcgagggt
     8281 tcggcactac aacgttcgcc ccggccagac catccggagg gggcaaaaga cgactatcag
     8341 gagtcgccac cacgccccaa tgcatattgt cggtgcatac attgtccaca agcagcgctt
     8401 cggcgtgatg gaagaacagc atgccgctca accagttatc agagcagcga ttgcgtacaa
     8461 gctcaggacg gcttccccga tcccgtactg cgataccaaa atgatgattg ccgcggcaga
     8521 cgttgtctgc gacacggggc tgcgcacctg caaagacaaa gatgcccgat tcccggttgc
     8581 aactgacctc attgtcgata aaggccggcc gacattcagg cccgtaaatc actacgccag
     8641 ataaaatgcc ctctgtggcc cggcacgacg tgatcgtgca ggaagaatcc aacacattga
     8701 tggccgaatg ttggtcgctt cccacatacc ggaatgtcag gccagaaatg cggccaccag
     8761 ggacccgctg cagatagagc ggccccccga gtcgtgaata gatctggacc gcgtctcgcc
     8821 ccgcccccgt taacaaaacc ggccgagcag aaataaatat tttgtcttca taaatgccgg
     8881 cccggacaaa cacttggtcc tgatcaccgg cgtccttcaa cgccgcgctc ggccgaggat
     8941 agacattagg atcttgccaa tcgacaatta atgttttccc cccgagcgga ttcggcggag
     9001 gacttgatgt cgccggctgg gattccacgc tcatcgcttg ctcaccccca cattggatca
     9061 atcagagttt tcgccccatc atgtggaaca acccgtttga gcttgtcaag tctccggggc
     9121 gtacaaaact ggtcgtgtaa ggccttatca atcaaccggg ccgtctgaac gcccgacgaa
     9181 gcacccgtta ttgcatcagt ttttccctga ccggcgtacc ccgccgtggc atgtggattg
     9241 cgacttggtg tcatggcttg gcaggggccg cggacactgg aggcgcatcg atgaaaacac
     9301 tcgcacttgt tccactggga atcctcgcaa gtctggtcat gatcgcggga caccccacga
     9361 agaccctggc cgctccatca caagttcagg acacttctcg tcatctttac gaccgcgtga
     9421 tggaagagtt caaacatcgg gactatgaag ccgcactggc cggttttcgt ttgttcctcg
     9481 aactgcacgg ccaaacttcg ttggccgcga atgctcagta ctgggtcggt gagtgtcaat
     9541 tccgtatggg acgatacaag gaagctttga atgcgttcta caacgtcgtg tcctactacc
     9601 cgctgagtca aaaactggca gcctccacat tgaagatcgg gcaaacctac acaagactca
     9661 aggatcacga aaaggcccgg atgatgtatg agcgagtggt tgaccagtat ccggacagtc
     9721 ccgaagccga ggtagcgaga aaagccatcg aggtggaggc ggccaaaatc gagccttcac
     9781 tgcctccagt gaatgagtga cggtctccgc gcgtcactcg agatcactca cacccaaaag
     9841 ctgctcgagt tcctgaaccg tgtaggaggg ttgtgctttc aacgtcgtca ctcggatgcc
     9901 ggccgagtga aggatccgct gaacttcgcg accgttggtg gatctatccg gatcggttgg
     9961 agactcctcg tccaactgca cgacctgttc aaccacacgc gagcccggat gaacgagggc
    10021 aaaatcagca cgtttaagcg ccaggtgtcg cagcacctgg atccgcgcgt cgcctgccgc
    10081 ttctaccatg agaaatttcc agaggggaac ttgagcgaac accaggtagc ggtcctccac
    10141 ggcgagtcga ataagattgt agaggagtaa ctccttctcg gccagtagcg gctggggccg
    10201 caatactacc cccaacggga gagtaaatgg tcccgcggaa tgtgccttgc ctgaccatcc
    10261 ggaaacaatc cgccacaaca ggaacaccag tacagcagca atgagtgcaa tgaagggcgt
    10321 atcaattgca gcttgtctca tgcgcgaaaa tgcttcatcc atcccggggc ggccggcacg
    10381 atcgcaccta acagcacagg atgcttcgct ccggtcaccg aaggaatgtt gccggactga
    10441 ctcgtcaccg tcagatacgc aagcacggca aaggccactg cttcgaacgc tttgctgtcc
    10501 catcccaatg agtcaaaggt gctgaccgga acggccccaa acactgcggt cagttgatcc
    10561 atgacggccc gattgcgtac accgccgccg ccgaccacca cctcatcgat agtgccttta
    10621 atccatcgcc gggctgtgcc gactgccttc gcagtccata gactgcaggt cgccaggaga
    10681 tcctcgatcg acagacctcg ttgtttttgg actgagacga gttcatcaat gagtgaaggg
    10741 ccgaaggctt ctcggcctgt tgacttaggc ggacggcgag aaagaaaggg atgagcaagc
    10801 agtttcccca gtagtcgcgg gtccgctcgc cctttcaggg ccagcttgcc gtcacggtcc
    10861 atcgcgatac gtccattggt cgctcggttg accaggccat ccaacaccat gttggccggg
    10921 ccggtatcga acgcgagaac gccgctccat tcccctccac gaggaacgta tgtgacgttg
    10981 ctgatcccgc caagattgac gatcagtcgc gagcgctgtg gatgtctcaa cagcaaggca
    11041 tggactgccg gggttagcgg cgcgccttgc cctcccgccg ccatatcgcg cggacgaaag
    11101 ttcgccacgg tggtgattcc cgtccgctcg gcgatgacgg ccggttccgc tatctgaagc
    11161 gtggagcgga tcgcgccggc gcctcccgcg tgaattccct gaggaagatg atgcacggtt
    11221 tgaccatgcg agccgatcac cgcgatgtcc gccgggctca actttgcttg gcgaatgacg
    11281 cttagtgccg catcggcaaa ccattcgccc aacagggcat tgaggtgaca aatttccgca
    11341 acggtacctg agacggatgc agcaagaatc cgttgttgga gagatcgagg gtaggaaagg
    11401 gtctgcgccg cgagtgcccg ggcgcggagc gatagccctc gaccggagac ttctacaagc
    11461 gccgcatcaa cgccatcacc ggacgtccct gacatcaggc ctacaatctt cattcgcttc
    11521 ctctgcaccg tgggactgct acgctaatag aacttcagcg cctggtcaag ccgcacacaa
    11581 ccaattgagc catgcggacg cgttgacatc caagaaacct cgatgctacg gtccccgccc
    11641 atgttctccg gatctttctg gacgtggtcc ttcgcaatcc tggcgacttc ctggctgacg
    11701 tgctccgctt ccgtcgcggt tgcgacgtcg tccgagcccg taaacgtggt cgtcacgatt
    11761 ccggtcctca aagatctggc cgagcaagcg ggaggaccct acatccgcgt cacctcactg
    11821 ctgaaaggct atgagaacga acacacctat tcgccgaagc cgagcgatct tatcgcggtg
    11881 cgaaaagcca gactgttgtt tgaagtcggt ctagggctcg aggtatgggt gaccaatctc
    11941 gtcaagaacg ccggcagcgc ctctctgcgg attatcacaa cctcaaaagg gatcggcatc
    12001 atgcgggacg atcaggagcc tgtcgaggca caccacagcc tggcacatga acaggccaat
    12061 ccccacgtat ggatggaccc cgagaacgcc gcgacgatgt tgcggcacat caccgacgca
    12121 ctgatcgaag tggatccgga ccatgcgcac gagttccgtc agaaccaggc agcctacctc
    12181 aagcgcttgg atctcattcg ggatgagctg accgagcgtg tccgcaagct gccggacagg
    12241 cgtttcgtgg cgcatcatcc cgcctggcca tactttgcca gacgcttcgg ttttcagatc
    12301 gcgggaacca ttcaagcaca atcaggtagc gaaccttcgg caatgcacat ccagcatctt
    12361 atttcgacca tcaagaaaca gcggattagg gcggtggtat cggaaatcca actgagccag
    12421 aagcttcccg agctcttggc aaaggaaacg ggcatccgga tcgtcgtact caccacactt
    12481 cccggcggta tcccccaaac ggagacgtat ctcgacatgc tccgttataa tgtgctccaa
    12541 ttggccaacg cgctcgaggc cgcttgatct cggccgcccg gtgcgatcgt ttcccggagc
    12601 gccggaggga cacacctctc gtgtcagcat cctcagacac tatcattcaa tttgaccacg
    12661 ccacattcgg atttcctggc gtcatcgctt tgaaggatct ttctcttgca attcaagcgg
    12721 gggagttcat cggggtcatt ggaccgaacg gttcaggcaa gaccactctc tgcaaagccg
    12781 ttctcggcct gatggcgccc gttgagggga atcttcgcat cttcgattgt gcctgcgatg
    12841 aactgcgttg tcaccatcgt gcgaagattg gatatttgcc gcagaagggc gtcgtggatc
    12901 ggaatttccc ggtgacggtg ctcgagacgg tcatgatggg acgttatgga gctatggggc
    12961 tgctcaaacg gcccggcacg accgatcgtc tgatcgctat ggaagcgctt acccatgtcg
    13021 gaatggaacg gcacaaacat acggcattgg gccatttgtc cggaggccag caacaacggg
    13081 tctttattgc ccgtgccctg gcgcagcggc cacaggtgtt gctgctcgac gagccgacga
    13141 caggtctcga catgacgact caacacaatg tcatcgaact gatcgagcat cttcaccagg
    13201 aattgcgcct cacagtcctg cttattaccc acgatatcaa catgattcgc tcacgagtgg
    13261 atcgcttgat ccttctgaag acccgcctct atgccgccgg tcccccggcc gaggtgctca
    13321 agcccgacat cctccgtgaa gtgtacggca aagacctcgt gatcaccgac aaggacctga
    13381 tcattgtcga agactaccat caccaccact aaggattaat aacaaagcct gtgctggagc
    13441 tcctttccta cgacttcatg cagcgctcat tgttcgccgc agcgatggtc ggagcgctct
    13501 gctcggtcat cggtgtcttt gtcgtcttgc gcgggctcgc tttcgtcggg gcagggacgg
    13561 cccatgcggc atttgcgggc gtggctttgg cctatttgat tggctggccg ccgttggtgc
    13621 tcgcgattct ttttggactc gtcaccgtct ggatcacagg ctgggtcgaa gaaaaaggga
    13681 cgatgaagct cgatgtgtcg atcgggattt tatacaccac gacgatggca ctggcgatcc
    13741 tcttcatcgg cttgatgaaa acgtacaatg ccgaggtgta tggctacctc tttgggaacg
    13801 tcctcgccgt gaccgctgag gaactgcgaa cgatctctgc tttgagcatt gtcgtgctgg
    13861 gtattattct gttgttctca aaggaattgt atttcatcgc gttcgatcag gagatggcag
    13921 aagcgtccgg tgtgccggcc aggatgatgt ttttcttgct gctgactctc gttgccttca
    13981 cggtggtcgt atccctcaaa actgtcggtg cgattttagt gtttgccatg gtactgatcc
    14041 ccgcatcgac cgcctatcag ttgacacata gtctctcgac gctgacttgg tattccgtga
    14101 taattggaat cacgtgtgcc gtaggtggtg tgttgctctc ggcggtatgg gacatcccgt
    14161 cagggccggc catcgtgctc ctgtccacct tcctattctt cctgtcagtg gtgctctcgc
    14221 ccaaacggct acgtcgttcc attagcaacg tgtagacaca gctgccattg aaaatttgct
    14281 tgccttctga cttgagttca acgagtattg attttacgta gctgacagga gataagtagg
    14341 tacattcgtc attcttacgg acaggaggtg ctatgaggtc ggtgatacag cgaaggttga
    14401 acgggatgcg tatcttgtgg atgggtctgc tggtgacagg tctttggatt aactctgcct
    14461 atgccgtcga tatgaaaatg caggacggcg gtattgaacc tgggaaggtc gtattgggta
    14521 tgagagcggg ttttgcgccg ttgacccagc aattgacgcc cggtacgtcc acagatgtcg
    14581 ggtccctcgt caactttcaa gccatgtaca gcctcaataa ctggttcctg atcggcatga
    14641 tggttgaatg ggaacgtcac agcatcagta ctgagggggg ttttgacata ggacaccagg
    14701 atacggtgtc ggtgttaccg accgttgaat tgcgccctgt tcggttcggc cccatcagtc
    14761 cctacgtcaa tatgagcttt ggcgtcaacg tgaatagctt cggagaggac agagccatcg
    14821 ctggcaatcg catttcaccg agtaatacgt ttgcctggcg ccttggatgg ggagccgact
    14881 ggatgattac aaaacaattc gcgttgaacg gagaaatggc ctacaagcgc aatgacggac
    14941 acgcaaccgt tacaggtccc ggtcccgttg gtggtggccg cgtggatgat tggaatgcgt
    15001 cttcgtttgg attcttgttt ggcgtaaaaa tgttcttctg atgatcactg aatctgcgca
    15061 gagtctgctt tacgcagctc tgcgcaggtc acgcgaggag agaaccagag aagttattcc
    15121 tgcctggcct gcttcgagcg gctccagacc attcccgtct gctccttgga actaaaaccg
    15181 agttttccgt agaacgacgg gcgacgcgtg cacagccaaa acagctcgac gcgtttgagc
    15241 cgaggatggt tgagaatgcg ttgaacgatg tccgagccga tcccttgctt ttggtaagcc
    15301 ttgtctacga ttacgtccca gatcgtggct cgatacacga aatccgtcag cacgcggccg
    15361 aatcccacga gttgatcgcc atcccaggcg cacaacgcga ggtcggtatg gcgcagcata
    15421 tccttggcat cgtccagcgt gcgatccttt gcccatgggg cctgatgaaa aagccgcaac
    15481 agctgtgacg cttcaagttc tttcttctcg gagaatgtaa cagcggtctt gagggcggcg
    15541 ggcatcttat actagagtat cctcgcaact caggggatga tgggccttag tgtacgagga
    15601 atgcgatggc aacgcaagtc cgtagctgcc ccaa
//
LOCUS       DASTQY010000104         7956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1220898, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000104 DASTQY010000000
VERSION     DASTQY010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220898"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VFR82_03340"
     CDS             <1..968
                     /locus_tag="VFR82_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500661.1"
                     /translation="DGESVATFLHEHSNPFDLLVTADVPSVVEFGAGDLSFATELADL
                     YAPRICEHNRTLLLHCIDRLSPQSKLGGPLHPSQDRLQALRSRADVSFQFFGDQDMCG
                     FDELAEAGRLASRYAVAVCWAPATPTFAYEPTRLSPEVIQRELERTKGPYRHAQYAGE
                     PALEVQNGDRTLLFPPWKFDIHGPLVLLNLLARGGHLGVLGAVDNQVFWELLSQLLED
                     ERYRPENEPFTPENLPVIFNTVYEPLATLPIGESLELASCGTLRRSIPRVLPSQRLPE
                     IYRFRSVLIRRGACFPGVPASYTARRFSDMVEEASPWMMTLVPAD"
     gene            1097..2038
                     /locus_tag="VFR82_03345"
     CDS             1097..2038
                     /locus_tag="VFR82_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU4500662.1"
                     /translation="MNVPKPFQAIQDNIARVIKGKDHVIEMAIVCLVARGHLLLEDVP
                     GVGKTTLAHSLARSLDCSFKRIQFTSDLLPSDIVGVSIFNRQHQAFEFVPGPIFANIV
                     LADEINRTTPKTQSSLLEAMSEAQVSVDNQTHPLKQPFMVIATQNPAEYHGTFPLPES
                     QLDRFLMRLRIGYPSLEEERKVLNRPYALHPADSLAPVLTAQDVLELQRRIEEVHMDE
                     SVSEYLLAIVHATRHSELLSLGVSTRGALALNNASKALALVRGREYCLPDDVKELAPI
                     ILSHRVMLSRAQGMRAQSFEQAERIVLDIVETIPVPV"
     gene            2053..3204
                     /locus_tag="VFR82_03350"
     CDS             2053..3204
                     /locus_tag="VFR82_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU4500663.1"
                     /translation="MPVTKAFGHIPAVRIRSRLHYLATRRSIRLTSEGVRFLLFTIGI
                     GVAAINTGNNLFYLLLAMMLGLVVISGLLSEHCLRRLEFHRHLPDLIMANDPTTVTLS
                     VTNHNRWLPSFSLRLSDVVSGQQLDRGLFIDHLAAQRSVLLSYPIVASTRGKLYLDCV
                     RAETLFPFGLFLKRALYQVPAEIPVSPPIRPVSLRFVDEMVSEGQGLSLPRRGHGTQL
                     YNLRLYQPGDDSRAIHWMTTARTSTLMVRETEAEDQRRITIALSTLAPDERNTLFEHS
                     VTLVASLLWHLSQRSYPLRLIVHEADSGLGCGSDHLLAMLRLLALCERRAPDSAGPTL
                     FNPVLTLGHDAERGYTVVVLPWSDPEAPAAVSADRVLHAAHIEGLTHGF"
     gene            3194..5371
                     /locus_tag="VFR82_03355"
     CDS             3194..5371
                     /locus_tag="VFR82_03355"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEU4500664.1"
                     /translation="MVFNQACRFSTIVLAATALAGLLFARSVPIWLAILTLTVLVFAI
                     LQTFGWPAAKTVLSGLPAPPSVWNSLLIGAFVLFLLDLTVISRELLPAGIHFLVVLLN
                     VKLMTLHDRRDYRHLYAISLMAILASAALTTDVWYVSVFVVYLLAAVWSLLLYHLTGG
                     PLAANPAVAQVSSRGTSFITSRFFWLTNGIAVLTFLFTLVIFFILPRISVGVLQKPKG
                     EGLRTTGFSERVDLGMIGSVKEDRQIVMRVELPDQTDTGKERFYLRGLAYDQYNGRSW
                     SSASRQRRSLGSIADGVFAVRSGANRTGYGLSEPLRQDILLEALDTSVLFAAPFAEYV
                     SGDFIGIHVDSMAGLHLPYTSSSRIRYSVVSREHRISSEDRAAAELEYASAIRDRYLQ
                     IPEGSDQVRDLTRRVTESAATPHERTTAVLNHLLSEYQYSLDADTTTSLHPIDDFLFR
                     RKTGYCEHYATAMVIMLRTLGVPARLVTGFLATEWNEFGGYYTVRQRDAHAWVEVFYP
                     HSGWITLDPTPTAASIPSPSIWEGFQRLGESIRLYWDRAVIRYSARDQLAVIHSLREG
                     SDSARDAVSRWTVALGAAALQLMRTCIGQVQTSHPIMAWALLFALVIALSALILAVRH
                     RWSPGHFSRGIPVRRQQQIVQHYRSMLRIAAQRGIRIAPSTTPTELTQHVRQSWSEAE
                     AAVVELTALYCRGRFGNSSLSGEELLRAVDQIRALQRLARASQ"
     gene            complement(5615..5689)
                     /locus_tag="VFR82_03360"
     tRNA            complement(5615..5689)
                     /locus_tag="VFR82_03360"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5655..5657),aa:Gly,seq:gcc)
     gene            6205..>7956
                     /locus_tag="VFR82_03365"
     CDS             6205..>7956
                     /locus_tag="VFR82_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU4500665.1"
                     /translation="MFLSRRQFLKVSAGTVAAVAVADKVLALTALQPVIEVGNPLGEY
                     PDRSWERVYHDQYRYDSSFTWCCSPNDTHGCRVRAFVRNGVVMRVEQNYDHQTYEDLY
                     GNRGTFAHNPRMCLKGFTFHRRVYGPYRLKGPLMRKGWKEWMDAGAPELTPDVKRKYK
                     FDARFLDDMMRVSWDTAATYAAKGCILIATRYSGEAGARRLREQGYAPEMIEMMKGAG
                     VRCFKHRAGMPVLGIIGKMMNTRFNGGVLPLLDSWIRKVDPDRAQGGKYYSNYTWHGD
                     QDPSHPWWNGTQNCDIDLSDMRFSKLNTSWGKNFVENKMPEAHWKLESIERGARIVVI
                     TPEYNPTAYRADYWIPVRPQTDAALFLGACKLILDENLQDIDYIKGFTDMPLLVRTDT
                     LQYLDPRDVIKDFAFPDFSKSYSGRIQSLKPDQIERLGGMMVWDLNKKQAVALHREQV
                     GYHFVQSGIDPALNGTYRVKLLNGREVDVSPIYQMYQVHLQDYDLDTVAQITRAPKDL
                     IVRWGRDSGTIKPAAMHNGEGVCHYFHMTEMGRAAALVVTLTGNIGKFGTGCHTWSGN
                     YKAGIWNATPWSGAGLAV"
BASE COUNT         1620 a         2379 c         2192 g         1765 t
ORIGIN      
        1 tcgatggcga atcggtggcg acttttcttc atgaacattc gaaccctttt gacttgcttg
       61 taacggcaga cgtcccttcc gtggtggaat tcggcgcggg tgatttatcg tttgccaccg
      121 aactcgccga cctgtacgcc ccccggatct gtgagcataa tcgaacgctc ctgctccatt
      181 gcatcgaccg tctcagtcca cagtccaaat tgggcggtcc gttgcatcca agccaggacc
      241 ggttgcaggc gcttcgttcg agagccgacg tctcgtttca atttttcggg gatcaggaca
      301 tgtgcggctt cgatgagctg gccgaagccg gacggctcgc gtctcgatac gccgtggccg
      361 tctgttgggc tcccgcgact cctacatttg cctacgaacc cacgcggctt tcccctgaag
      421 tgattcagcg cgaactggaa cgaacgaagg gaccgtaccg tcacgcgcag tacgcaggtg
      481 agccggccct cgaagtgcaa aatggagatc ggacgctcct gtttccacca tggaagttcg
      541 acatccatgg ccccctcgtc ttattaaatc ttctggcgcg cggcggccac ctgggcgttc
      601 taggtgcggt cgacaatcag gtgttttggg aactcctgtc gcaactgttg gaagatgaac
      661 ggtaccgccc agaaaacgag ccctttactc ccgaaaatct gcccgtcatc ttcaacaccg
      721 tttacgagcc gcttgcgaca ctgccgatcg gcgaaagcct cgagctggca agctgtggaa
      781 cacttcgtcg tagtatcccg cgcgtcttgc cctcgcaacg actgccagaa atctatcggt
      841 tccgttccgt gttgattcgg cgtggcgcat gctttcccgg cgtgcctgcc agctatacgg
      901 cgagacggtt ctctgacatg gtcgaagaag cctctccctg gatgatgaca ctcgttcccg
      961 ctgactaaaa tctcccttcg gggcggtcat caccgcgtaa attcgtcgaa aaattgaccg
     1021 tcaatagacc ctttgttaca cttcagcggt gtcacacaac cacatattct ccagccacta
     1081 gagccaataa tctgacatga acgtccccaa accttttcaa gcgattcagg acaatattgc
     1141 ccgagtcatt aagggcaagg accatgtcat tgagatggcc atcgtgtgtc ttgtggcgcg
     1201 cggacatttg ctgctcgaag acgtaccagg agtcggcaag accacccttg cccatagctt
     1261 ggcccgttcg ctcgattgct cgttcaagcg gattcaattt accagcgatc tcctaccctc
     1321 cgatattgta ggggtgtcca ttttcaaccg ccagcatcag gcatttgaat ttgtcccagg
     1381 gccgattttt gcgaatatcg tgctggccga cgagatcaat cgcacgacgc ccaagacaca
     1441 aagcagcctc ttggaggcga tgagcgaagc acaagtctcc gtggacaacc aaacgcatcc
     1501 cctgaaacaa cccttcatgg tcatcgccac acagaatcct gccgaatatc acggcacgtt
     1561 cccactgccc gaatctcaac tggatcgatt tttgatgcgc ttacgcatcg gatatccgtc
     1621 attggaggag gagcgcaagg tccttaaccg cccttatgcg ctgcatcctg cggacagcct
     1681 cgcgccggtg ctcactgcgc aggacgtgct ggaattgcag cggcggatcg aggaggtgca
     1741 tatggatgag agtgtcagcg aatatctgct ggctattgtc catgcaacaa ggcacagcga
     1801 gctcctgtct ctcggcgtca gtaccagagg agccttggca ctcaacaacg cctcgaaggc
     1861 cctggcactt gtgcgcggcc gcgagtattg cctgcccgac gacgtcaaag aattggcccc
     1921 gatcatattg tctcatcgtg tcatgctaag ccgagcgcaa ggtatgcggg ctcaaagctt
     1981 tgaacaggcc gagcgcattg tcctcgacat tgtcgaaacg attcccgtcc ccgtgtgatg
     2041 ctgcaggacg gcatgcctgt gacgaaggca ttcgggcaca ttcccgccgt ccgcatccga
     2101 tcccgcctgc actatctggc aacccgccgt tccatccgcc tcacctctga aggcgtccgg
     2161 ttcttactgt tcaccatcgg catcggcgtc gcagccatca acacaggcaa caatcttttt
     2221 tatctcttgc tggcgatgat gctcggcctc gtggtgattt ccggcctcct gtccgagcac
     2281 tgcttgcgcc ggctcgagtt tcatcgccat ctccctgatt tgatcatggc gaatgatcca
     2341 acgaccgtta cgctctcggt cacgaatcac aaccgctggc tgccgagttt ctctctacgc
     2401 ctgtccgatg tggtgagcgg acagcaactt gaccggggcc tgttcattga tcacttggcg
     2461 gcgcaacgct ctgtcctact ttcttacccg atcgtagcga gcacccgcgg caagctgtat
     2521 ctcgactgcg tgagagcgga aacgcttttt cccttcggcc tgtttctcaa gcgggcactc
     2581 tatcaggtcc ccgccgaaat tcccgtcagt ccgccgatca gaccggtctc tcttcgcttc
     2641 gtcgatgaaa tggtcagcga gggacaaggc ctgtcgcttc cccgccgcgg acatggaacg
     2701 cagttgtaca acttacggct ctatcagccc ggcgatgatt cgcgggcaat tcattggatg
     2761 accactgccc gaacctctac cctgatggtc agggaaaccg aagccgagga tcaacggcga
     2821 attacgattg cgctttcgac gctcgcaccg gacgagcgaa atacgctgtt cgaacacagc
     2881 gtgaccctgg tcgcatcgct tctctggcac ttgtctcaga gatcctatcc gctccgcctg
     2941 atcgtccatg aagcggattc cgggctggga tgcggttctg atcatctgct cgccatgctg
     3001 cgtctactcg ctctttgtga acgacgtgct cctgattccg caggtcctac actcttcaat
     3061 cccgtcctga cattgggcca cgacgccgag cgcggttaca cggttgttgt cctgccgtgg
     3121 agcgatcccg aggctccagc ggctgtgtcc gccgatcgcg tgcttcatgc tgcccacatt
     3181 gaaggactca cccatggttt ttaaccaggc ctgtcggttc agtacgattg tcctggcggc
     3241 gacggcgttg gccggattgc tgttcgcacg cagcgtgccg atttggctcg ccattctgac
     3301 actcacagtc cttgtcttcg cgatcctaca gacgttcgga tggccggcgg ccaaaacagt
     3361 cctcagtggc ctccccgctc caccgtccgt ttggaacagc ctgttgattg gcgccttcgt
     3421 tttgtttctg ctggacctga cggtcatatc gcgcgagttg cttccggcag gcattcactt
     3481 ccttgtcgta cttctgaacg tgaagctcat gacgctgcat gatcgccgcg actaccgtca
     3541 cctctacgcc atttccctga tggcaattct ggcatcggcc gccttgacga cggacgtctg
     3601 gtacgtatct gtttttgttg tctatctgct ggcagcggtg tggagtctgc tgctgtacca
     3661 tttgaccggc gggccactcg cggccaatcc ggccgtcgcg caggtctcgt cgcgcggcac
     3721 ttcgttcatc acgagcagat ttttctggct gaccaacggc atcgccgttc tcacctttct
     3781 tttcaccctt gtcattttct tcatcctccc ccggatcagc gtgggcgttc tgcaaaagcc
     3841 caaaggagag ggcttgcgga caacgggatt ttcagaacga gttgatctcg gcatgattgg
     3901 ctcggtgaaa gaagatcgcc aaattgtgat gcgcgtagag ctgccagatc agacagacac
     3961 gggtaaagag cgattctacc tgcgaggtct tgcctacgat caatacaacg gccgatcatg
     4021 gagctccgct agccggcagc gtcgaagtct agggagcatt gccgatggcg tattcgcagt
     4081 ccgctccggc gccaaccgga ccggatacgg gctgtcggag ccgttgcggc aagatatcct
     4141 gctcgaggcc ctcgacacgt cagtgttgtt tgcggctccg ttcgccgagt atgtaagtgg
     4201 agactttatc ggcatccatg tggacagtat ggccggtctc cacctgccgt acacatcttc
     4261 gtcccgcata cggtattccg tcgtctcgcg cgaacaccgc atttcgtcgg aggacagggc
     4321 cgctgctgaa ctcgagtacg cgtcggccat acgagaccgc tatctccaaa ttcctgaagg
     4381 gtccgatcaa gtcagggatt tgacgcgccg agtcactgag tcagccgcca ctcctcatga
     4441 aagaaccacc gccgtcctca atcacctgct gtccgagtat caatacagtc tggacgcaga
     4501 cacgacgaca tcccttcatc cgattgacga cttcctgttt cgtcgaaaga ccgggtattg
     4561 cgagcactat gcgaccgcaa tggtcatcat gctccgcact ctcggcgtac cagcgcgttt
     4621 agtgacgggc tttctcgcaa cggagtggaa cgaattcgga ggctattaca cagtccggca
     4681 gcgcgatgcc cacgcgtggg ttgaggtctt ttatccgcat tccggatgga tcacacttga
     4741 ccccacaccg actgcggcct ccatcccatc accgtcaata tgggaagggt ttcagcggct
     4801 tggcgaatca attcgtctgt actgggaccg ggcagtcatc cggtacagtg ccagagatca
     4861 actggcagtc attcacagtc ttcgtgaagg cagtgattcc gcacgtgatg ccgtcagtcg
     4921 gtggaccgtc gcgcttggag cggccgcact ccaactcatg cgtacatgta tcggccaagt
     4981 gcagacttcg catccgatca tggcttgggc attgctcttt gccttggtca ttgccctgtc
     5041 agcattgata ttggccgttc gtcatcgatg gtcgcctgga cacttctcac gaggcatccc
     5101 cgtaaggagg cagcaacaga ttgtgcagca ctatagaagc atgctacgga tcgcagcaca
     5161 gcgcggaatt cgcattgcac cgtccacgac acccactgaa cttacgcaac acgtcagaca
     5221 gtcatggtct gaggccgaag cagccgtcgt cgagctcaca gcactctatt gccgggggcg
     5281 ctttggcaac tcgtcgctct ccggtgaaga acttcttcgg gccgttgacc aaatccgcgc
     5341 tctgcaacga ctcgcgcggg catctcagta gcgacactgc actggcggtc accttcgctt
     5401 cctctactct atttctccgt attctccgtc caaaccgaca gcatcacttt cacaggtccg
     5461 cggagtacct ctggagcctc caaacagcac ttgctgccct cttgcatgag gtccgaataa
     5521 cgctatgact ggaaaggaat gaaccgctgg aggtgtccat ccggcggcgc aatgaagaga
     5581 gcttcgccga tgatccgcag gcaagagcaa ggtttggagc gggaaaaggg atttgaaccc
     5641 tcgaccctcg ccttggcaag gcgatgctct accactgagc tattcccgct ctgaaaaggg
     5701 tggtggagga gtggcggatt ctactcacca aggctgggac tgtcaagcaa cgccaacggt
     5761 tacctgcggc gtctttttcc gttacaacgc ctctcctgcg ctacgaaatg gtaacgcttc
     5821 atgctattac cataagccat taataaaata agaatttatt gcaatctgac cggtgcctac
     5881 ctgttgagcg atacacctga tacccatccg gccaaggaag agagcctcat gaggcgacct
     5941 ttaacagcca ttttattgca tcatttcgcc tagataccgt aatagcactc aatatcttaa
     6001 gcccgccggt tatggtgcgc atcttgccaa taagatggcc ctatgatgct gcaaacctca
     6061 tggaccttgg accaagtcag gtacctgctt catacgggca accggcatca gattttgaga
     6121 cgaagggagg tgggacaagc agtggttgta ttcggtgtag gcacgtgggc tctaggggag
     6181 ccctcaactt aggaggtgtc accaatgttt ctgtcacgca gacagttttt aaaagtctcc
     6241 gctgggacgg tcgccgcggt ggcggtggcc gataaagtcc tggcgttgac cgctctgcag
     6301 ccggtcatcg aggtgggcaa ccccctcggg gaatatccgg accggtcgtg ggagcgggtc
     6361 tatcacgacc agtatcggta tgattcgtcg tttacgtggt gttgttcgcc gaacgatacc
     6421 cacggctgcc gcgtccgagc gttcgtccgg aacggcgtcg tcatgcgggt cgagcagaat
     6481 tacgaccacc agacctatga agacctctac ggcaatcgcg ggacgtttgc ccataacccg
     6541 cgcatgtgct tgaaggggtt caccttccac cgccgggtgt acggcccgta tcgcttgaag
     6601 ggcccgttga tgcggaaagg ctggaaggag tggatggatg ccggggcgcc cgaattgacc
     6661 ccggatgtga aacgcaaata caaattcgac gcccgattcc tggacgacat gatgcgggtg
     6721 tcctgggaca cggcggccac ctatgcggcc aagggctgca ttctcatcgc gacccgctac
     6781 agcggggaag cgggagcccg gcggctgcgg gagcagggct atgcgccgga gatgatcgag
     6841 atgatgaagg gcgcgggcgt gcgctgcttc aagcatcgcg ccggcatgcc ggtgctcggc
     6901 attatcggca aaatgatgaa tacgcggttc aacggtggag tattgccgtt gttggactcc
     6961 tggatccgta aagtggatcc agaccgtgcg cagggcggca agtattattc gaactacacc
     7021 tggcacggtg accaggaccc gtcgcacccc tggtggaacg ggacgcagaa ctgcgatatc
     7081 gacctctctg acatgcgctt ctccaagctg aacaccagct ggggcaagaa cttcgtcgag
     7141 aacaagatgc cggaagcgca ctggaagctc gaatcgatcg agcgcggtgc ccgcatcgtc
     7201 gtgatcacgc cggagtacaa cccaacggcc tatcgggcgg actactggat tccagtccgg
     7261 ccgcaaaccg atgcggcatt gttcttaggc gcctgcaaac tcatcctcga tgagaacctg
     7321 caggacatcg actacatcaa ggggtttacg gacatgcccc tgctcgtccg taccgatacg
     7381 ctccagtatc tggatccccg ggatgtaatc aaggatttcg ctttccccga tttctccaag
     7441 agctactccg gccgcatcca gtccttgaaa cccgaccaga ttgaacggtt gggcggcatg
     7501 atggtgtggg acctgaataa gaagcaggcg gtggcactgc atcgggagca agtggggtat
     7561 cacttcgtgc agagcgggat cgatccagcc ttgaacggca cctaccgggt caagctgctg
     7621 aacggccgcg aagtcgacgt ctcgccgatc tatcagatgt atcaagtcca cctacaggac
     7681 tacgatctgg acacggtggc gcagatcacc cgcgcaccca aggacctcat cgtccggtgg
     7741 ggccgggatt cgggcacgat caagcccgcg gcgatgcaca acggcgaagg cgtctgccac
     7801 tatttccaca tgacggaaat ggggcgcgcc gccgcactcg tggtgacctt gaccgggaac
     7861 atcggcaagt tcggcacggg ctgccatacc tggtcgggca actacaaggc gggcatctgg
     7921 aacgccacgc cctggtcagg agccgggtta gccgtg
//
LOCUS       DASTQY010000105         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_67494, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000105 DASTQY010000000
VERSION     DASTQY010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67494"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFR82_03370"
     CDS             complement(<1..216)
                     /locus_tag="VFR82_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500666.1"
                     /translation="MSLPSVRVETIGWFTASCLLVSNIIGGGIFTTTGLMARELGDPM
                     LILLLWTVGGLFAVGGAMVYGELGARLP"
     gene            267..1262
                     /locus_tag="VFR82_03375"
     CDS             267..1262
                     /locus_tag="VFR82_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEU4500667.1"
                     /translation="MPEKQTKKPRGTGTRRRRKPAGQSTALTATELQAAAPHAEVADL
                     HRAIEQDGGKVLSVYREPYGGHWVVLSALPIELVEPTPYQRNLSDTHVRKLEAVISKI
                     ARFLDPIICVRVSRENQTAKYWTPNGNHRLSAMRTLGARSIVAIVVPETAAAYHILAL
                     NTEKAHNLREKALEVIRMYKELMKLPETEEETYALEFEEPALITLGLCYEERPRFSGG
                     AYHPVLKRVEEFLKKPMRTALESREARARKLLELDDAIVKQVNALKAKGLTSPYLKSF
                     VVARVNPLRFRQKDAPPVSFDEAMERMIQAAAKFNPEKVKVDDLAKSGGMPDESE"
     gene            1333..1686
                     /locus_tag="VFR82_03380"
     CDS             1333..1686
                     /locus_tag="VFR82_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEU4500668.1"
                     /translation="MGLADNKCIPCRGGVPPLPTDRIQTLLQELGRGWALNPQGRLER
                     LYTFKDFAQALSFVNRVGAVAEAEGHHPDLYLSWGKCRVEIWTHKINGLTESDFYLAA
                     KADREFEPFRAMAGG"
     gene            1694..>2863
                     /locus_tag="VFR82_03385"
     CDS             1694..>2863
                     /locus_tag="VFR82_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HEU4500669.1"
                     /translation="MSHTSDSTRRATGEAQVALVNMPFSFSKYPSIQLGTLSSLLKAQ
                     GLAVDCHYLNVHFAHKIGVPLYELICEKRALFGEWLFSYLLFRENPKRAEYPRVFKPV
                     FEQMAQQSGRPIGYFEEMATRIAPQFLTWALTAFDWSRYKLIGFTSTFDQNVASLTMA
                     KLIKDLYPDVTIVFGGANYDGEMGLEYFRAFPFIDYVVVGEGEVAFPALVRQVLSEHS
                     DGVPPAVVFRQDGQIRLMPNTSLFTDFARTGPPDYDDYYRLLAELGAAAQGLDRILLY
                     EGSRGCWWGEKHHCTFCGLNAQSMKFRAKSPEQVAREMTYLSHRYDTARFRLVDNIID
                     MKYIDQLFGKFAGDHCDLDVFIETKSNLHKQQIRTLAAGGVKCMQPGLESLSATQL"
BASE COUNT          651 a          781 c          809 g          622 t
ORIGIN      
        1 ggggagccgc gcgccaagtt cgccatacac catcgccccg cccacagcga atagaccgcc
       61 aaccgtccac aacaagagga ttaacatcgg atcccccagt tctcgcgcca tcaagcctgt
      121 ggtcgtgaag atgccacctc cgatgatatt gctgacgagc agacaactgg cggtaaacca
      181 cccgattgtt tcaacacgca cactcggcag cgacatggtt gaggcctttc ttttgtcgag
      241 tgactctcct accatatcat ccgtccatgc cagagaagca gacaaagaag ccacgcggga
      301 ccggcactcg gcgcagacga aagccggcag ggcagtcgac tgccttgacg gcaacagaac
      361 tgcaagccgc agccccgcat gccgaagtgg ccgacctgca tcgagccatc gagcaggatg
      421 gtggcaaggt cctttcggta tatcgggagc cttacggagg ccattgggtg gtgttgtcgg
      481 cgttgcccat cgagctggtt gaaccgactc cgtatcagcg caacctgtct gatactcatg
      541 taagaaagct tgaagcagtg atcagcaaga tcgcccggtt tcttgatccg atcatttgcg
      601 tacgcgtctc tagggaaaat cagacggcga agtattggac gccgaacggc aatcatcggc
      661 tctccgccat gcggaccttg ggcgcgagaa gcatcgtggc gatcgtcgtg cccgaaactg
      721 cagccgccta ccacattctc gctcttaata ccgagaaagc ccataacctc cgggaaaagg
      781 cactcgaggt cattcgcatg tacaaggaac ttatgaaact cccggaaacg gaagaagaga
      841 cctacgcgtt ggagttcgaa gaaccggcac tcattacgct cggtctctgt tatgaagaac
      901 gaccacgatt cagtgggggc gcctaccatc ctgtcctgaa acgtgtggag gaatttctta
      961 aaaaaccgat gcgtacggca ctcgagtcgc gggaggcacg agcgcgcaag cttctcgagc
     1021 ttgacgatgc catcgtgaaa caagtcaatg cgcttaaggc gaaggggttg accagtccct
     1081 acctgaagag cttcgtcgtt gcccgcgtaa atccgctccg ttttcggcaa aaggacgctc
     1141 cccccgtgtc ctttgacgaa gccatggaac gcatgatcca ggcggcggcc aaattcaatc
     1201 cggagaaagt gaaggtggac gatctcgcaa aatcaggcgg gatgccggac gagagcgagt
     1261 agcgcgttcc gccttgtttc tatccgaaag cccctcgtac aataaccaga ctcatttaac
     1321 agggaggcca cgatgggtct tgctgacaac aaatgcatcc cttgccgcgg cggcgtgccg
     1381 ccgcttccca ctgaccgaat acaaacgttg cttcaagagc tcggtagggg ctgggccttg
     1441 aatccccagg gccgcctcga gcgattgtat accttcaagg actttgcgca ggcgttgtcc
     1501 tttgtgaaca gagtcggtgc cgtggccgag gccgaaggcc accatcctga tctgtatttg
     1561 tcgtggggta agtgcagggt cgaaatctgg acacacaaga tcaatgggtt gactgagagc
     1621 gatttttact tggcggcgaa ggcggacagg gaatttgaac cgttccgagc catggcgggc
     1681 gggtgatcac agcgtgagtc acacatcaga ttctacgagg cgtgcaacgg gcgaggcgca
     1741 ggtcgcgctc gtcaacatgc cgttcagctt ctcgaagtat ccctccatcc agcttggcac
     1801 actctcgtca ttgctgaaag cacaaggcct tgccgtcgat tgccattatt tgaacgtcca
     1861 ttttgcccac aagatcggtg tgccgctgta cgaattgatc tgtgaaaagc gcgcactctt
     1921 cggtgagtgg ctgttttcct atctcctctt tcgtgagaat cccaagcgcg ccgaataccc
     1981 gcgtgtcttc aagcctgtct ttgaacagat ggcgcagcag agtggccgac cgatcggcta
     2041 ttttgaggag atggccacga ggatcgcccc gcagtttctg acgtgggctt tgacggcgtt
     2101 tgattggagc cgatacaagc tgatcggctt cacctccacg tttgatcaaa acgtggccag
     2161 tctgacgatg gccaagctga tcaaggacct ctatcctgac gtcaccatcg tgtttggcgg
     2221 agcgaactac gacggagaaa tgggtttgga atatttccgg gcgttcccgt ttatcgacta
     2281 tgtggtcgtt ggcgaaggag aagtagcctt tcccgctctt gtaaggcagg tgttatcaga
     2341 gcattcggac ggtgtaccgc cggcagtcgt cttccggcag gatggacaga tccggttaat
     2401 gcccaatacc tctctcttta cggatttcgc acggacaggt ccgccggatt atgatgatta
     2461 ctatcgtttg ctagctgaac tcggcgcggc ggcgcaaggt ctggatcgca ttctcttgta
     2521 cgaggggtcg cgcggttgtt ggtggggaga gaaacatcat tgcacctttt gcggactcaa
     2581 tgctcaaagc atgaagtttc gtgcgaaatc tccggaacag gtcgcgcgtg aaatgaccta
     2641 cctctctcac cggtatgata cggcgcggtt caggctggtt gataacatta tcgatatgaa
     2701 atacatcgac cagctctttg ggaagtttgc cggcgaccat tgcgatcttg atgtctttat
     2761 tgagaccaag agcaaccttc acaaacagca gattcgaacg ttggccgctg gcggtgtcaa
     2821 atgcatgcag cccgggctgg aaagtctgag cgcgacgcag ctg
//
LOCUS       DASTQY010000106         8463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1083884, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000106 DASTQY010000000
VERSION     DASTQY010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8463
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083884"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFR82_03390"
     CDS             <1..378
                     /locus_tag="VFR82_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEU4500670.1"
                     /translation="SDKGAIVEGAGFTLYDMESIKGSDADRVERDPICDRSKRPKIHK
                     VEPDEAKPGQKVTIKGENFGTKECFHGVAFSAAGPAKIEYKFVNDSTIEATVPDVKAG
                     MSFIDVVAGGGNARSKGFLVQTK"
     gene            549..1994
                     /gene="accC"
                     /locus_tag="VFR82_03395"
     CDS             549..1994
                     /gene="accC"
                     /locus_tag="VFR82_03395"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU4500671.1"
                     /translation="MGTSEPEPEMFKKILVANRGEIAMRIIRACRELNIATAAIYSEA
                     DSTGIYVKKADEAYVVGPGPVKGFLDGQQIVDLARRIGADAIHPGYGFLSENAEFAER
                     CGAAGITFIGPSPRAITLMGSKVKARELAQSVGVPIVPGTEGGVSEASAALAFARQVG
                     YPVIIKASAGGGGRGLRVVRSDAELRENMEVAAREAQAAFGDGSVFLEKYIERPHHIE
                     FQILADRHGHVIHLGERDCSIQRRHQKLIEIAPSLILTPVLRREMGEAAITIAKAVNY
                     DNAGTVEFLLDQEGRFYFIEMNPRLQVEHTVTEQITAIDIVRNQIAIAAGKPLDIRQE
                     EVTLQGHAIQCRINAEDPKNNFMPCTGTVTAYLSPGGIGVRIDGAVYKDYTVPPYYDA
                     LLAKLTVRGRTWEETVSRMRRSLEEYVLRGVKTTIPFMKTIMQDQDFLAGRFDTSYLE
                     THPDLFMYDEFEQPEDLVVAISAAIAAYEGL"
     gene            2029..3987
                     /gene="oadA"
                     /locus_tag="VFR82_03400"
     CDS             2029..3987
                     /gene="oadA"
                     /locus_tag="VFR82_03400"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HEU4500672.1"
                     /translation="MAPKRPSRKKPAAKKRPLGSWATKPSVRPTARLQELAIQPAAGK
                     PILITDVALRDGHQSLLATRMRTEDMLPIAQKLDAVGFWSLEVWGGATFDTCLRFLKE
                     DPWERLRALRAAMPNTKLQMLLRGQNLVGYRHYADDVLERFVERSAANGIDVFRIFDA
                     LNDVRNVDRAVREVKACGKHAEAALSYTVSPVHNIDRYVDLARQLEDLGTDTLCIKDM
                     AGLLAPLDAYHLVRRLKAAVKVPIHLHSHYTSGMASMSALMAILGGLDMLDTAMSPLA
                     GGTSHPATESMVASLQGTPYDTGLALESFLPITEHFRTVRRRYRQFESDFTGVDAEIL
                     TSQIPGGMLSNLAAQLTEQNALDRMKEVLDEVPRVREDMGYPPLVTPTSQIVGTQATL
                     NVLTGERYKVITTETKNYFLGLYGRAPGQVDRNIMARAVGNEEPIRTRPADRLDPELE
                     AVRKDLPASAQSMEDQLSFVLFPAIARDFFEAREKGDLVPEPLEAAAPKGPAVAGELH
                     LAPVEFNLTVHGETYHVKVSGSGRKTDGRKPYYIRVNDKLEEVSLEALQEVLAGVPEA
                     QDTGKDAKPKRPKPSKPGDVAPPMPGRVVKVLVEKDQRVKAGDPLLIIEAMKMESRVP
                     APLDGKVVAILVSEGENVKTDETVIQLE"
     gene            4056..4937
                     /locus_tag="VFR82_03405"
     CDS             4056..4937
                     /locus_tag="VFR82_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU4500673.1"
                     /translation="MPACSSPLPIPPYFEAFERLPIHTVQVKGETMAYLDLGQGQAVI
                     LIHGFGGSMWQWEHQQIPLSSKFRLITPDLLGSGLSAKPEIDYRPEQTLEYFIGFMDA
                     LKIERATLVGNSMGAGLAIGMALAHPNRVSDLILIDGLPANVREKLTSPSIKRALDSS
                     APTWLVSLGNWLFGGVMVESVLKEIVHDPSLLTPAVIERSNRNRQRPGLMRPLMTVRD
                     TLPLWETGFAKRIPDIGHRTLVIWGEEDRVFPLSAGEELQQSIKGAVLARIPQAGHIP
                     QWERPDVVNRAMIEFLR"
     gene            4979..5632
                     /gene="tpx"
                     /locus_tag="VFR82_03410"
     CDS             4979..5632
                     /gene="tpx"
                     /locus_tag="VFR82_03410"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU4500674.1"
                     /translation="MTTTGMRLWLLVPCVSALMLTGCSGGHDFTYKNITVADGSAKAG
                     DGHSVLFKGSPLSLSGNGIKVGDQLRSVKLAQPDLSLVNVADTGGKGKVRIISVVPSL
                     DTKVCEQQTHYLSEKNKGLDKMVELITVSIDTPFAQKRFAEEAKIANVTFLSDYRNGD
                     FGKAYGLFLKDPHVLARAVMVVDAQNTVRHLQITPELAQLPDMDEAFQVARKLITAS"
     gene            5718..7130
                     /gene="sthA"
                     /locus_tag="VFR82_03415"
     CDS             5718..7130
                     /gene="sthA"
                     /locus_tag="VFR82_03415"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HEU4500675.1"
                     /translation="MSHYDLLVIGTGPAGQKSAVQAAKLGKKVGVIERHEVVGGVCIN
                     TGTIPSKSLREAVLYLSGFRQRNLYGASYRVKATITIEDLAFRTNHVITHEVAIVRDQ
                     MARNRVDMIHGKASFVDPHRLRIQQTTGTIDHTADFIVIAVGTEPARPTEVPFDDESV
                     IDTDGLLRLKRIPDSIVIVGGGVIGTEYASILAAMGVPVTLIDKRPRLLEFVDGEIIT
                     ALQEQMKEIGVTLYHEEEVLGITKSPDHHIGVRLKHSKPIQATTLMYAVGREGATKEL
                     NLEAVGLKPDERGRLFVNAHFQTAIPHIYAAGDVIGFPALASTSMQQGRHASCHAFGF
                     PDRMDTDLLPYGIYAIPEISMVGKNEEELVRTEVPYAVGIARYREIARGQLIGDETGM
                     LKLLFHRQTRELLGVHAIGEGATELIHIGQAVMAYHGRVDYFIDTVFNYPTLAECYKV
                     ASLDGINRLPRPWPPANHKP"
     gene            7203..7871
                     /gene="tenA"
                     /locus_tag="VFR82_03420"
     CDS             7203..7871
                     /gene="tenA"
                     /locus_tag="VFR82_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU4500676.1"
                     /translation="MSFSNHLRQLAQSIWKAQLSHPFVVALGNGTLPEGKFRYYILQD
                     ARFLGDLARVFSAGALRAPDAESALRFTKLAEETIVVERSLHEGYGTKWNMTAQQMTS
                     VPMAPTNYAYTRHMLAVAQSGSAAEITVVALPCAWIYCVVGHHLLRHGPPKKQHPYRD
                     WLMLYASPEFAEVQQWMRKKVDQWAKTAGKAEQRRMEESFVISSRYEWMFWEMAWNEE
                     QWPV"
     gene            8187..>8463
                     /locus_tag="VFR82_03425"
     CDS             8187..>8463
                     /locus_tag="VFR82_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500677.1"
                     /translation="MTYRQITINLAALVFLLALLGLVYWQHTGARAVPRLIDALQSGD
                     LQTQMLAAQGLRHIGQPAQAAVPVLIELAIGQGRSSIQIEAAGALPPI"
BASE COUNT         1846 a         2521 c         2432 g         1664 t
ORIGIN      
        1 agcgacaagg gggccattgt ggagggggcc ggcttcacac tctacgatat ggagtccatc
       61 aagggttcag atgcagaccg ggttgaacgc gatcccatct gtgatcgttc gaaacgtccg
      121 aagattcaca aagtggaacc ggacgaagcc aaaccaggcc aaaaggtgac gatcaagggc
      181 gaaaacttcg ggacgaaaga atgcttccac ggcgtcgcat tcagtgccgc aggacctgcc
      241 aagatcgagt acaagttcgt caacgactcg acgatcgaag ccacggtgcc cgacgtgaaa
      301 gccggcatgt cattcatcga cgtggtagcc ggtggtggta acgcccggtc caagggcttt
      361 cttgtgcaaa ccaagtagaa tttgatcatc tcgagggggc gcggatagcg ctcccttgcg
      421 agttcacccc tgagcgtttt tccatccctc cgtgccgatt agtcatccct cgctccctcg
      481 acattctttt cgatcttatg ctagcttacg cctcctttgc gccggtctgc ggtctcatgg
      541 agccgcgcat gggaacatcg gagcccgaac ctgaaatgtt caagaaaatc ctcgtggcca
      601 atcgtggtga gatcgccatg cggatcattc gcgcctgccg tgaactcaat atcgccactg
      661 ccgccatcta ttccgaagcc gattcgaccg gcatttacgt caagaaagcc gacgaagcct
      721 acgtcgtagg tcccggacca gtgaaggggt tcctggacgg gcaacaaatc gttgatctcg
      781 cgcgtcgcat cggagccgac gccattcacc ctggctacgg ttttctgtca gaaaacgcgg
      841 agtttgctga gcggtgcggt gcggctggca ttacgttcat cggcccctcg ccgcgcgcga
      901 tcacgctgat gggtagcaag gtcaaggctc gtgagttggc ccaatcagtt ggcgttccca
      961 ttgttcccgg gaccgaagga ggcgtgagcg aggcatcggc cgcgctcgcc tttgcccggc
     1021 aagtcggcta tccggtaatc attaaagcga gtgccggcgg cggcggtcga ggactcaggg
     1081 tcgtgcggtc ggatgcagag ctgcgggaaa acatggaggt cgccgcacgc gaagcgcagg
     1141 ctgccttcgg cgacggcagt gtctttctcg agaaatacat cgaacggccc catcatatcg
     1201 agtttcaaat ccttgccgac cggcatgggc atgtcattca tctcggcgaa cgggactgtt
     1261 ccatccaacg gcgacaccag aaactgattg aaatcgcccc gtcgttgatt ctgacgcctg
     1321 tactgcgccg cgaaatgggt gaagccgcga tcacgatcgc gaaagccgtc aactatgata
     1381 acgccgggac cgtcgaattc ctgctcgatc aggagggtcg cttctacttc atcgaaatga
     1441 atccccgcct gcaggtcgag cacacggtga ccgagcagat cacggcgatc gacatcgtgc
     1501 gcaaccagat tgctattgcg gccggcaaac cgctcgacat tcggcaggaa gaagttacgc
     1561 tgcagggcca cgccattcag tgccggatca acgccgagga tccgaagaac aactttatgc
     1621 cctgcaccgg cacggtgacc gcttacctat cgccgggtgg gatcggagtc cggatcgatg
     1681 gcgccgtcta taaagactat accgtgccgc cctattacga tgccttgctg gccaagctga
     1741 ccgtccgcgg ccgaacctgg gaagaaacgg tcagccgcat gcgtcgatcg cttgaagaat
     1801 acgtattgcg cggtgtgaag acgaccatcc cgttcatgaa aaccatcatg caggaccagg
     1861 actttctcgc cgggcgtttc gacacttctt acctggagac gcatcccgac ctcttcatgt
     1921 acgacgaatt cgagcagccg gaagatctgg tggtggcaat ttcagccgca attgcggcct
     1981 atgaagggtt gtaagaccgg acggcgctca tccctctgag acgacaccat ggcaccgaag
     2041 agaccttcta ggaaaaagcc tgcagcaaag aagcgtccgc tcggctcgtg ggccacgaaa
     2101 ccctccgtcc ggccgacagc tcgtctgcag gaacttgcca tccaaccggc tgcgggtaaa
     2161 cccatcctca taaccgacgt cgcccttcgc gatgggcatc aatcgctcct ggccacccgc
     2221 atgagaacgg aggatatgct gccgatcgcc caaaaactgg acgcggtcgg gttttggtcg
     2281 ttggaggtat ggggcggtgc gaccttcgac acatgcctcc ggtttctcaa agaagatccc
     2341 tgggaacggc tgcgcgcgct tcgggcagcc atgcccaata cgaagctcca gatgctcctg
     2401 cgaggacaaa acctcgtggg ctaccgccac tatgccgatg acgtgctcga gcgatttgtc
     2461 gaacgttcgg cggccaacgg aattgacgtc tttcgcatct ttgacgcgct caacgacgtg
     2521 cgtaatgtgg atcgggcggt ccgtgaagtg aaggcctgcg gcaagcatgc cgaggcggcg
     2581 ctcagttata cggtgagccc tgtccacaat atcgatcgtt acgtcgacct cgccaggcaa
     2641 ttggaagatc tcggcaccga tacgctctgc atcaaagaca tggcaggtct gctggccccg
     2701 ctcgacgcct atcatctggt gcgccgcctg aaagccgccg tcaaagttcc tattcacctg
     2761 cattctcact acacatccgg catggcgtcg atgtctgcct taatggcgat cctcggcgga
     2821 ctcgacatgc ttgacacggc catgtcgccg ctggccggcg gcacatcgca ccccgcgacg
     2881 gaatccatgg tggcttcgct gcaaggcaca ccgtatgata cgggcctcgc cctggagtca
     2941 ttcctgccga ttaccgagca cttccggacc gtccgtcgcc ggtaccgcca atttgaaagc
     3001 gactttaccg gcgtcgatgc ggagatcctg acctcgcaga ttcctggcgg tatgctgtct
     3061 aatctcgcgg cgcagttgac cgaacagaac gcgttggacc gtatgaaaga agtgctcgac
     3121 gaagtgccgc gggtgcgcga ggacatgggc tatccaccgc tggtcacgcc gacgagccag
     3181 attgtcggca cacaggccac cctcaatgtc ctgacgggcg aacgctacaa agtcattacg
     3241 accgagacga agaactactt tctcgggctc tacggccgtg cgccagggca ggttgatcgg
     3301 aacatcatgg cccgtgcggt tggaaatgaa gagccgatca ggacgagacc ggccgaccgc
     3361 ttggaccccg agttggaagc ggtcaggaaa gatctccctg cttccgccca aagcatggaa
     3421 gatcagctct ccttcgtact gtttccggcc attgcgcgtg actttttcga ggcgcgggaa
     3481 aagggagatc tggtgccgga accgctcgaa gccgcggcac caaaaggacc ggccgtggcc
     3541 ggagagctcc acttggcgcc tgttgaattc aacctgaccg tgcatggaga aacctaccat
     3601 gtgaaggtat cgggatccgg gcgcaaaact gatggacgca aaccgtacta catccgcgtc
     3661 aacgacaaac tcgaagaggt gtccctcgaa gccctccaag aggtcctggc aggcgttccc
     3721 gaggcgcagg ataccggcaa agatgccaaa ccgaaacgac cgaagccgtc caaacctggc
     3781 gacgtcgccc ctccaatgcc cggacgcgtc gtcaaagtcc tcgtggaaaa agatcaacgt
     3841 gtcaaggccg gcgacccact gctgatcatc gaggccatga agatggaaag ccgcgtgccg
     3901 gcgccgctcg acggcaaagt cgtcgccatt cttgtcagtg agggcgagaa tgtgaagacc
     3961 gatgaaaccg tcattcaact tgaatagcgc gattcgtggc cggcagactg ctgaacagga
     4021 tatgtatcgc ttttgagctc gctgcggtca tcgtgatgcc ggcttgttcg tctcctcttc
     4081 cgattccccc ttacttcgaa gccttcgagc gtctccccat ccataccgtg caggtcaaag
     4141 gcgagacgat ggcatatctc gatttgggtc agggccaggc ggtgatcctg atccacggat
     4201 tcggcggatc catgtggcaa tgggaacatc agcagattcc tctctcgtcg aaattccggc
     4261 tcattacccc ggacctcctc gggtcaggcc tctctgcgaa accggagatt gactaccgcc
     4321 ccgagcaaac attggagtat ttcattggct tcatggatgc gctcaagatc gagcgggcaa
     4381 cactagtcgg caactcaatg ggcgccggcc tcgccatcgg catggcgctg gcgcatccga
     4441 accgcgtgtc cgacctgata ctgatcgatg gcctgccggc gaatgtgcgg gagaagctca
     4501 cgagcccgtc gatcaaacgg gcgctcgaca gcagcgcccc gacctggctg gtctcactgg
     4561 gcaattggct gtttggcggt gtgatggttg aatcagtgtt gaaggagatc gtgcatgacc
     4621 cgtccttgct cactcctgca gtcattgagc gatccaaccg caaccgccag cggcccggcc
     4681 tcatgcgacc tctcatgacg gtccgggaca cgcttccgct gtgggaaacc ggtttcgcca
     4741 aacggatccc tgacatcggc caccggaccc tcgtcatctg gggtgaagag gatcgtgttt
     4801 ttcctctctc ggccggagaa gaattacaac aaagtatcaa aggagctgtg ctggctcgca
     4861 ttccacaggc ggggcacatt ccccaatggg aacgccctga tgtggtgaat cgagctatga
     4921 tagaatttct gcgataaatg gcctgagagt actcgatcac gacaacggga ggcttttcat
     4981 gacaacgacc ggtatgcgcc tatggcttct agttccctgt gtcagcgcgc tgatgctgac
     5041 cggctgcagc ggcggacacg acttcacgta taagaacatc acggttgcgg acgggagtgc
     5101 aaaagccggc gacgggcata gcgtgctatt taaaggcagt ccgctctccc tttcgggaaa
     5161 cggcatcaaa gtcggagacc agctgcggag cgtgaaactt gcccagcctg acctctcgct
     5221 cgtcaacgtg gccgataccg gtgggaaggg aaaggtccgc atcatcagcg tcgtgccgtc
     5281 gctcgacacc aaggtgtgtg agcagcaaac tcattacctg agcgaaaaaa ataagggcct
     5341 ggacaagatg gtcgaactga tcacggtcag cattgatacg ccctttgccc agaagcggtt
     5401 tgcggaagag gctaagatcg ccaacgtcac atttttgtcc gattatcgta acggcgactt
     5461 cgggaaggcc tacgggctct tcttgaaaga tccccatgtt ctggcccgtg ccgtcatggt
     5521 cgtcgacgca cagaataccg tccgccattt gcagatcact ccggaactcg ctcaattgcc
     5581 cgacatggat gaagcctttc aggtcgcgcg aaagctgatt accgcgagct gaggcggaca
     5641 gtcgcgcccg ggtgaaggcc ggctcaacgg ctcgcgctca aatccattag tgcctcacct
     5701 ttcactcgag ggtctggatg tcccattacg accttctcgt catcggcacc ggacctgccg
     5761 gccagaaatc cgccgtgcaa gccgccaaac tcggcaagaa agtcggtgta atcgagcgcc
     5821 atgaagtcgt cggtggcgtg tgcattaata ccggcaccat accaagtaaa tcgttgcgtg
     5881 aagccgtgct ctacctttcc ggttttcgcc aacggaatct gtacggcgcg agctaccgcg
     5941 ttaaagcgac gatcacgatc gaagacctcg ccttccgcac caaccacgtc atcacacatg
     6001 aagtcgccat cgtcagggat cagatggccc gcaatcgtgt cgatatgatc cacggcaagg
     6061 ccagttttgt cgatccccac cgattgcgga ttcagcagac gaccggcacg atcgatcaca
     6121 cggcggattt tatcgtcatt gccgtgggca ccgaaccggc cagacccacg gaggtgcctt
     6181 ttgacgacga gtcggtgatc gatacggatg gcctgctcag gctcaaacgg atcccagact
     6241 ccatcgtcat cgtgggcggc ggggtcatcg gcactgagta cgcgtcgatt ctcgccgcca
     6301 tgggcgtccc ggtcactctc atcgacaaac ggccgcgcct gctcgagttc gtcgacgggg
     6361 agatcatcac ggctcttcaa gagcagatga aagagatcgg cgtcacgttg taccacgagg
     6421 aggaagtgct tggaatcacg aagtcgcccg atcaccacat cggcgtgcgc ttgaagcaca
     6481 gtaaaccgat tcaagccacc acgctcatgt atgcggtcgg ccgagaaggc gcgaccaaag
     6541 agctcaatct ggaggcggtc ggactgaagc ccgatgagcg aggccgtctg ttcgtcaacg
     6601 cgcatttcca aaccgccatc ccacatatct atgcagccgg tgacgtgatc ggcttccctg
     6661 ccctcgcgtc aacgtccatg cagcagggcc gccatgcgtc gtgccatgcc tttggttttc
     6721 cggaccgaat ggacaccgat ctcttgccct atgggatcta tgcgattccg gaaatctcga
     6781 tggtgggaaa aaacgaagaa gagttggtcc gcaccgaggt tccctacgcc gtgggcatag
     6841 cgcgttatcg cgagattgcc agaggccaat tgatcggcga cgaaacgggc atgctgaaac
     6901 tcctctttca tcgccaaact cgcgagctgc tcggcgtcca tgcgatcggc gaaggggcca
     6961 cggagcttat ccatatcggc caagccgtca tggcctacca tggccgcgtc gactacttca
     7021 tcgacaccgt gttcaattat ccaacattgg ctgagtgcta taaagtcgcc tcattggacg
     7081 gcatcaaccg gctgccaagg ccttggccgc ctgccaatca caaaccctga cgcgcaccgc
     7141 gtgaagcgtt ctgatcgcgt agtcgatttg ggaccatgcg ggctcggcca aaaaggagaa
     7201 ccatgtcgtt ttccaaccat cttcgccagc tagcccaatc gatctggaaa gcccaactgt
     7261 cccatccctt cgtcgtcgcc ttgggcaatg gcaccttgcc ggagggaaag ttccgctact
     7321 acatcctgca agacgctcgc tttctcggtg atctcgctcg agtattctcc gccggcgcct
     7381 tgcgggcacc cgacgcggaa tcggcgctcc gattcaccaa gctcgccgaa gaaaccatcg
     7441 tggtcgagag gagtctacac gagggatacg ggaccaagtg gaacatgacg gcccaacaga
     7501 tgacctccgt tcccatggcg ccaaccaact atgcctacac ccgtcacatg cttgccgtcg
     7561 cccaatcagg atcggccgca gagattaccg ttgtcgctct gccctgcgcc tggatttact
     7621 gcgtcgtggg acaccatctg ctccgccacg ggccgccgaa gaaacagcac ccgtatcgcg
     7681 actggctcat gctctatgcg tcgccggaat ttgccgaggt gcaacaatgg atgcgcaaga
     7741 aggtggatca gtgggccaag acggctggaa aggcggaaca gcgtcgcatg gaggaatcgt
     7801 tcgtgatcag ctcacgctat gagtggatgt tctgggagat ggcgtggaac gaggagcagt
     7861 ggccggtgta gccctggctc gcccagggat gagtgccctt gtgctcgccg aacgcggcac
     7921 acctgactaa tcgtatttgg aaataaaggg cggtgctcgt ccaggcgcgc acgttcggca
     7981 tctcatcccc ggactcgcga tacaaccgac cgcaaggtgg gagagaaata gaaacggcag
     8041 gtgcggttac ccgagcctcg tgcaagtcgc ctggcaggag gtagcgcccc gcttattggg
     8101 tagcgacacc ggccataggg agagcaagga agagaaaaga atctaattca tagtccgttt
     8161 cgccaaaaaa ctttgtgcgc ataaacatga cgtaccgtca aatcaccatc aatcttgctg
     8221 cgctggtatt cctactggcg ctgctaggtc ttgtctactg gcagcatacc ggtgctcgag
     8281 cagtgccccg gctcattgac gccttgcaaa gcggtgacct tcagacgcag atgctggcgg
     8341 cacagggatt gcggcatatc ggacaaccgg cccaggcggc cgttcccgtt ctgatagaac
     8401 tggctatcgg gcagggcagg tcttcaatac agatcgaggc agcaggcgcc ttgcctccca
     8461 tcg
//
LOCUS       DASTQY010000107         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_586116, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000107 DASTQY010000000
VERSION     DASTQY010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586116"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="VFR82_03430"
     CDS             <1..1141
                     /locus_tag="VFR82_03430"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU4500678.1"
                     /translation="FEPFSMEGYRSKGAGQVRGFSPERYLDSVQAERVDRYAQFALVA
                     AKEALADSGLQMAREDPRRVGVIVGAGMGGMVMGEREITQLYQHQRPHRVHPNFIPVI
                     TLNSASGIVAMAHGAKGPNYTVSTACSSSAHALGQAMQCIKEGRADVVITVGADASIT
                     PLVFAGFCSLRALSTKYNDQPERASRPFDRLRDGFVMGEGAGALVLESSVHAKKRRAR
                     IYAEIAGYAATSEAYHMVIPREDGEEIATTMRLAMADAGLTPANIDYINAHATSTTIG
                     DAVETKALRQVFRTRADKLAINATKSLVGHTLGAAGAIGGIATVLAIHTGQIHPTANY
                     EEPDPACALRGLSSSVQERKVKAALLNAFGFGSNNAAVVIKRFTA"
     gene            1172..1468
                     /locus_tag="VFR82_03435"
     CDS             1172..1468
                     /locus_tag="VFR82_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU4500679.1"
                     /translation="MSDRAVTEKIITALADYLRRNPESIEPAHHLRDDLGLDSMAVIE
                     LLYKIEEAFDIQIPDEDLVRLSTVEAVCEYVEHRLAPAKRPVARSRAAKSRKKK"
     gene            1474..>2321
                     /gene="lpxD"
                     /locus_tag="VFR82_03440"
     CDS             1474..>2321
                     /gene="lpxD"
                     /locus_tag="VFR82_03440"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEU4500680.1"
                     /translation="MGSLSTPTTLTLSQIHALVGGTLHGNGASLLSSLASLDDATPQA
                     LAFISNDKAAKVPGGIKAGGLLVHRHLPEIDVPQIVVPNPLLAFAQVAQTFFIRPSAP
                     RGIAADVSKGTDVTIGADPSIWPFVTLGDRVSIGARVTLYPGVFVGADCVVGDDSVLY
                     PNVVVREGCSIGARAIIHSGTVIGADGFGYVQHQGRHQKIPQLGGVTIEDDVEVGANV
                     TIDRATLGRTIIKQGTKIDNLVQIAHNVTVGEHDIIVAQVGIAGSTTIGHHVMIGGQA
                     GLADHLT"
BASE COUNT          496 a          726 c          677 g          422 t
ORIGIN      
        1 tttcgagccc ttttctatgg aggggtaccg ctcaaagggt gccggccaag tgcgcggctt
       61 ttccccggaa cgatatctcg acagcgtgca agccgaacga gtggatcggt acgcacagtt
      121 tgcactggtc gccgcgaagg aagccctggc ggactccggc cttcagatgg ctcgcgagga
      181 tccgcgccgg gtcggcgtca tcgtaggtgc cggcatgggc ggcatggtca tgggagaacg
      241 ggaaatcacc caactctacc aacaccaacg ccctcaccgc gtccatccca attttattcc
      301 ggtgatcacg ctcaactcgg cgtcgggcat cgtggccatg gcgcatggag ccaaggggcc
      361 caattacacc gtgtcaaccg cctgctcatc cagcgcccat gcgctggggc aggccatgca
      421 atgcatcaag gaaggccgtg ctgacgtcgt cattaccgtc ggcgctgatg ccagcatcac
      481 gccattggtc ttcgcaggat tttgctccct tcgagcgctt tcgaccaagt acaacgatca
      541 gccggagcgc gcctcgcgtc ccttcgaccg gcttcgagac ggattcgtga tgggtgaagg
      601 cgccggcgca ctcgttctgg aatcgtccgt tcatgccaaa aaacgccgcg caagaatcta
      661 cgccgagatc gcgggatatg ccgccaccag tgaggcctat cacatggtca ttccccgcga
      721 agacggggaa gagatcgcca cgaccatgcg cttggccatg gccgacgcag ggctcacgcc
      781 tgcgaatatt gattacatca acgcgcacgc gacatcgacc acgatcggag acgcggttga
      841 gacaaaagcc cttcgtcaag tctttcgcac acgcgcggac aaactcgcca tcaatgcaac
      901 gaagtccctg gtcggccaca cactgggagc cgctggcgcc atcggcggca tcgcaaccgt
      961 tctggccatt cacacggggc agatccatcc aaccgccaat tacgaggagc cggatcccgc
     1021 ctgcgcattg cgaggcctta gctcatcggt gcaggaacgg aaggtcaagg cggcgctgct
     1081 caacgcattc ggcttcggca gcaataacgc cgccgtcgtc atcaagcgtt ttaccgcctg
     1141 aaagcaagca ccccgtaaag gacagagccg gatgagcgac cgcgcagtca ccgagaaaat
     1201 catcacagcc ctcgccgact atctgcgacg gaatccggag tccatcgaac cggctcatca
     1261 tttgcgcgac gacctgggtc tcgactccat ggccgtcatc gagttgctct ataagatcga
     1321 agaggccttc gacatccaaa taccggacga agacctggtc cgcctgtcga ccgtcgaagc
     1381 cgtctgcgag tatgtggagc accgactggc accggccaag cggccggtgg ctcgtagccg
     1441 tgcggccaag agtaggaaaa agaaatagcc tccatgggat ccttgtctac gcctaccacg
     1501 ctgacgctgt cccagattca tgcactggtg ggcggcacgc ttcatggaaa cggcgcctct
     1561 ctgctgtcat cgctggccag tctggatgac gcgaccccgc aagcattggc cttcatctcc
     1621 aacgacaaag ccgccaaggt cccgggcggt atcaaggccg gaggattact cgtacaccgc
     1681 cacttgccag aaatcgatgt tccgcaaatc gtcgtaccca atccactcct cgcctttgcg
     1741 caggtggccc aaaccttttt tatccggcct tccgctccgc gaggtattgc agcagacgtc
     1801 tcgaagggga ccgacgtcac gatcggggcc gatccctcca tctggccctt cgtcacgcta
     1861 ggagaccgcg tgagcattgg agcgagggtg acgctttatc ccggcgtctt cgtcggggcc
     1921 gattgcgttg tgggcgacga cagcgtgctc tatcccaacg tggtcgtccg ggaaggctgt
     1981 tcgatcggcg ccagagccat cattcacagt ggaacggtga taggagcgga tggctttgga
     2041 tacgtccagc atcagggccg acatcagaag atcccacagc tcgggggcgt cacgatcgag
     2101 gacgatgtgg aagtaggcgc caacgtgacg atcgatcgcg cgacgctcgg tcgtacgatc
     2161 atcaaacagg gcacaaagat cgacaatctc gtgcagattg cacacaatgt caccgtgggc
     2221 gagcacgaca tcatcgtggc tcaagtgggt atcgcaggca gtacgacgat cggtcaccac
     2281 gtcatgatcg gagggcaggc cggactagcg gatcatctga c
//
LOCUS       DASTQY010000108         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1703827, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000108 DASTQY010000000
VERSION     DASTQY010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703827"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VFR82_03445"
     CDS             <1..323
                     /locus_tag="VFR82_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500681.1"
                     /translation="VAEAMVYHSHNYTVSQEFQRYIDLGVFFGKERWLLNEFGKPEGE
                     GAKFIRSEWSFLLSHGLIYLLPVSVLRAAAKLLGYRLGLFHPFLPSWMLKKLSMHGMR
                     RSDV"
     gene            333..1727
                     /locus_tag="VFR82_03450"
     CDS             333..1727
                     /locus_tag="VFR82_03450"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEU4500682.1"
                     /translation="MLKRHMQFLQSLLFLFDLTVICVCWLTAYYLRFLQGFAPIEKPI
                     PPFEMYVWLVVPILAVWGISFRAFNLYRPRRMGTHLEEFFDIAKANTLAVLMLVAVTY
                     FMRQFDYSRLVFLYFWMLNLATLGFSRMAFREGLRFMRKQGFNQRHCLIVGAGKLGQR
                     VARSLALHSEFGIKTHGFLTRQPNKIGQMYEGCLVIGFYSDIEKHAPGMDIVFLCLPP
                     GAEDYSEKILQYLATTSIEVKVIPSIYEFITLRAEAEMFDGLPLITLQGSPLYGWNGV
                     VKRMTDLVGAAIALTLTAPLMALIVTLIKATSSGPVLYKQERMGLDGKPFFMLKFRTM
                     CADHPGDTNLLTSRDDPRVTPIGRLLRKTSLDELPQFWNVLMGDMSIVGPRPERSWVV
                     EAVRKQIPLYMLKHKMKAGITGWAQVNGWRGATSLDKRIEYDLYYIEHWSPWFDVKIM
                     CMTLWRGFLHKHAY"
     gene            1805..2845
                     /gene="rfbB"
                     /locus_tag="VFR82_03455"
     CDS             1805..2845
                     /gene="rfbB"
                     /locus_tag="VFR82_03455"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU4500683.1"
                     /translation="MNILVTGGAGFIGSHLVRRLVVQSGHYVVNLDALKYSGNRANTQ
                     DLDGHARYRFVHGDIADQQLVNGLLREHGIEGVINAAAETHVDRSIVDPGSFAKTDVV
                     GTGILLEEARRAGVKRFLQVSTDEVYGSVEAGMSREEDVLTPRSPYSASKAGGDLLTL
                     SYWTTYRFPVMVSRGSNTYGPHQYPEKFIPLFVTNALDDQPLPLYGDGRQRRDWLAVQ
                     DHCAAIEQILLNGEPGEVYNVGGGNERENIGVAEQLLALLGKPKSLIRLVEDRPGHDR
                     RYAVDCRKINRMGWQPTVSFDAGLKATVDWYRQNESWWRPIKSGEFRAYYEAMYAKRL
                     KEGLSPSSSCAS"
BASE COUNT          661 a          747 c          833 g          760 t
ORIGIN      
        1 acgttgctga agcaatggta taccactcac ataattacac cgtgtctcag gaattccagc
       61 ggtatattga cttaggcgtc ttcttcggga aggagcggtg gcttcttaat gaatttggaa
      121 agcctgaggg tgagggcgcg aaattcattc gatccgaatg gtcgtttctt ctctcgcatg
      181 gactgatcta tttgctgcca gtaagcgttc ttagagctgc tgccaaactg ttgggatacc
      241 gcctcggtct ctttcaccca ttcttgcctt catggatgtt gaaaaaactg agcatgcatg
      301 ggatgcggcg gagtgacgtg tgagatagcg acatgctgaa acggcacatg caatttctcc
      361 aaagccttct ctttctgttt gacctcacgg tcatttgtgt ctgctggctg actgcatact
      421 acctgagatt tcttcagggc ttcgctccga ttgagaaacc cattcctcct ttcgaaatgt
      481 atgtatggct ggtggtcccc attttggccg tatggggaat ttcgttccgg gcgttcaatc
      541 tttaccgtcc tcgccgaatg ggaactcatc tcgaagagtt tttcgatatc gccaaggcca
      601 atacgttggc tgttttgatg ctcgtcgcgg ttacctattt catgcgtcag ttcgactatt
      661 cgcggctcgt tttcctgtat ttttggatgt tgaacctggc gactcttggc ttttcgcgca
      721 tggccttcag ggaagggctg cggttcatgc gcaaacaagg attcaatcag cgccattgct
      781 tgattgtggg tgcaggaaag cttggacaac gagttgcacg gtcccttgca ttgcacagcg
      841 agtttggcat caagacccat ggcttcttga cgcggcaacc gaacaagatc ggtcaaatgt
      901 atgaaggctg cttggttatc gggttctatt ccgacattga gaaacatgct ccgggaatgg
      961 atattgtgtt tctgtgtcta ccgcctggag cggaggatta ctcggagaag atattgcaat
     1021 accttgcgac cacttcgatt gaagtgaaag tgattccatc gatttacgaa ttcatcacat
     1081 tgcgagccga agccgagatg tttgatggct tgcctctgat cacgctgcag ggatcaccgt
     1141 tgtatggctg gaatggagtg gtcaagcgga tgacagatct cgtcggggcg gcgatcgcat
     1201 tgacgctgac tgctcccttg atggcgctga tcgtgacact catcaaggcg acgtcatccg
     1261 ggccagtcct ttataagcaa gaaagaatgg gcctcgatgg taaaccattc ttcatgctga
     1321 aatttcggac catgtgtgcc gatcatcctg gagacacgaa ccttctcact tctcgagatg
     1381 atccccgagt tacacctatc ggccgcctgt tgcggaagac gagccttgac gagttgcctc
     1441 aattctggaa tgtgcttatg ggagacatga gcattgttgg tccacgcccc gaacgttctt
     1501 gggttgtcga agcagtgcga aaacagattc ctttgtatat gctcaaacat aaaatgaagg
     1561 ctgggattac tggctgggcc caagtcaacg gctggcgagg tgctacgtca ctcgacaagc
     1621 gaattgagta cgatttgtat tacatcgaac actggtcgcc ttggttcgat gtcaaaatta
     1681 tgtgcatgac tttgtggaga ggtttcctcc acaagcatgc ctattaacgc ctgcatcatt
     1741 gaggtatcac ttccccatag gagtcattcg tccactcatt aatttcaagt cgaactgaat
     1801 tcttatgaac attcttgtca ccggcggcgc tggcttcatc ggctcacatc tggttcgtcg
     1861 tttggttgtt cagagcggac actatgtcgt gaacctcgac gcgctcaagt attccggcaa
     1921 ccgtgccaac acgcaggatt tggacggtca tgcacgctat cgattcgtcc atggggacat
     1981 cgccgaccag caactggtca acggtctctt gcgggagcac gggattgaag gcgtgatcaa
     2041 tgcggcggca gagactcatg ttgaccgttc gattgtggac cccggcagct tcgccaagac
     2101 agatgttgtg gggaccggga ttttgctcga agaggcgcga cgcgccggcg tgaagcgttt
     2161 tctgcaggtg agcactgatg aagtctacgg ctctgtggag gccggcatgt ccagggaaga
     2221 ggatgtgctc acacctcgca gcccctactc ggcgagcaaa gcgggaggcg atctcctgac
     2281 gctgagctat tggaccacct atcggttccc tgtcatggtc tcgcggggaa gcaatactta
     2341 cggcccgcat cagtatccag aaaaattcat tccgttgttt gtcacgaatg ccttagacga
     2401 tcagccgttg ccgttgtacg gcgatggtcg ccagcgacga gactggttgg ccgtacaaga
     2461 ccactgtgcc gcaatcgagc agatcttgct gaacggagag cctggagagg tgtacaacgt
     2521 cggcggcggg aacgaacggg aaaacatcgg cgtggcggaa caattgctgg ccttgctggg
     2581 caaaccaaag agcttgatcc ggcttgtgga agaccggcca ggtcatgatc gacgctatgc
     2641 ggtcgattgc cgcaagatca atcggatggg atggcagccc acggtctcgt ttgacgccgg
     2701 actcaaggcc acggtcgact ggtatcgaca aaatgagtcc tggtggcgtc cgatcaagtc
     2761 cggagaattt cgtgcgtatt acgaagcgat gtatgccaag cgtctgaagg aaggcctctc
     2821 accctcgtcg tcatgcgcat cctgattacc ggcgccaacg gacaacttgg tagcgcatta
     2881 cagcgtacct tcacggggga ggaactcgtt ctcaaggact tgcctgattt tgatctcacc
     2941 aacccaacct gtgaagacga gatacgagac gcgcgtccca cggtgatcat gcatgtcggg
     3001 g
//
LOCUS       DASTQY010000109        17527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_669222, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000109 DASTQY010000000
VERSION     DASTQY010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 17527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17527
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669222"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFR82_03460"
     CDS             <1..205
                     /locus_tag="VFR82_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bL17 family ribosomal protein"
                     /protein_id="HEU4500684.1"
                     /translation="SKDVVSKLFSDVAGRFKDRPGGYTRMVKTRRRIGDAAELVAIEL
                     VARAEVSTSKKPGSTPAKTDKTE"
     gene            320..958
                     /gene="lptC"
                     /locus_tag="VFR82_03465"
     CDS             320..958
                     /gene="lptC"
                     /locus_tag="VFR82_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEU4500685.1"
                     /translation="MPSAECYYRARLFSFEEPDGNIVWQRWAQRILLGLSLALVCFLV
                     YLLMTNSTASPPPTATAPGTINQADAIISKFTFTQTKGDKVQWQVDAQQARLYEQDKR
                     AILDDVAVTLFGVQGREMTVHGDEGTLNTETKNFLLANRSEPLVIYTQSGYTIYTNHL
                     AWTDQTREIRTQDPVHIIGHGLDVKGRGLLGHLDTEEFEVLEDVHVDVAPAS"
     gene            969..1499
                     /locus_tag="VFR82_03470"
     CDS             969..1499
                     /locus_tag="VFR82_03470"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEU4500686.1"
                     /translation="MSLPTAGQAAMGAASNGASDEALGTTVISQRMTFKNQQSQAVFD
                     GAVVLTRGTLIVYSDRMVVSFRNQEASDSKKASEPPKAGTAPAKGPDTVSNRAVSQIE
                     ATGRVKIERDAGNATCQKAVYYRDEDKIVLTGDPVAWDKGTRVSGKRITMFLAEDRTV
                     VEGGSHVRIEPEGGTK"
     gene            1496..2278
                     /gene="lptB"
                     /locus_tag="VFR82_03475"
     CDS             1496..2278
                     /gene="lptB"
                     /locus_tag="VFR82_03475"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEU4500687.1"
                     /translation="MTEAVRLAPETDGVARSSEPRAVLRADGLVKSFRARKVVKGVSV
                     EVSAGEVVGLLGPNGAGKTTIFDMIVGLTQPDEGSIRLNAEEITALPMYKRARNGIGY
                     LPQESSVFRRLSVQDNILAILEMLDYSRSERAERVDVLLGELDLGHIRTSMAYALSGG
                     ERRRLEITRALATNPTFMLLDEPFAGIDPIAVADIQQIITRLKGKGIGILITDHNVQE
                     TLSITDRAYIINEGLVLESGPPDVIVKSETARAVYLGERFTL"
     gene            2295..3815
                     /gene="rpoN"
                     /locus_tag="VFR82_03480"
     CDS             2295..3815
                     /gene="rpoN"
                     /locus_tag="VFR82_03480"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEU4500688.1"
                     /translation="MKLRLDLRLSQKLIMTPQLQQAIKLLQLSRLELQQSLAQHLMEN
                     PLLDELPEEVEENESTTAEEKAEEAAASASPATETSETDGSSTAEERDTADEVSAAGW
                     EEYFGSDRRTGGSEGQSSSSDDFPSYEQTVAKAASLEDHLLWQLSLSGLSERNKAIGR
                     LLIGNLDDDGYLRTPLSEIITGTDFTEAEAESVLRDIQTFDPTGVGARDLPECLLLQL
                     GHLGKSPMGSLGARPGALKGSIVEAVVLHHLKDLEKKQYAKVAKALDVTVAEIFQATK
                     VIEALEPKPGRPFSNTQNYVIVPDVFVVKNEGEWVVLLNDDGLPRMRISPYYKQMMAS
                     GEGGSTETKAYLDEKLRAAQWVIRSIEQRNKTIVKVVSSIVKFQEQFFEKGIQYLKPL
                     VLKQVAEDIGMHESTISRVTANKYMYCPQGMLELKFFFNAGLQRADQPSDMLSSVTVR
                     EMIKKMVAEEDANHPLKDEEIAARLRERQVLIARRTVAKYRAEENIPPATQRKRFF"
     gene            3864..4367
                     /gene="raiA"
                     /locus_tag="VFR82_03485"
     CDS             3864..4367
                     /gene="raiA"
                     /locus_tag="VFR82_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU4500689.1"
                     /translation="MKITGRQMDVTSALKRYIRSRFERLERYDVSPDRLEIVLSVTKH
                     QHTAEVVCSIKGKRFQAKTSTTEMYATIDQLVNRLDKQIRKYKERRIAHKGRKKTQLP
                     APVGAPADLNDLKIVRPKIAVLSRKEARDRLDDDPGAMVLFTCVDSGKLQILRRSETG
                     HVVLIDP"
     gene            4378..5244
                     /gene="rapZ"
                     /locus_tag="VFR82_03490"
     CDS             4378..5244
                     /gene="rapZ"
                     /locus_tag="VFR82_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU4500690.1"
                     /translation="MPGLKLVVITGLSGAGKSHALKCFEDVGYFCVDNLPPALLPTFV
                     ELCHQQGGEIKNVALGIDIRERVFFADLTGILQRIKDLGHSVELLFLEARDPVLVRRF
                     SESRRPHPLLPELPVLEGVRFEKERLAEIRAHADRIIDTSNLSVHDLRDLLSRHFSQE
                     LSARRLTISLITFGYKFGVPYDIDLLFDVRFLRNPYFVPDLKPLTGEDPRVRSYVLDD
                     PDAKGFLDRLEQFFTYLIPLFERERRSYLNVGIGCTGGRHRSVVIAKRLQESFSSAGH
                     HVTLSHRDLTKP"
     gene            5488..6600
                     /gene="pilM"
                     /locus_tag="VFR82_03495"
     CDS             5488..6600
                     /gene="pilM"
                     /locus_tag="VFR82_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEU4500691.1"
                     /translation="MLNSLKSLVDKDIFSLLSPKRQLVGLDIGSSGIKLVQLKENRGR
                     YVLQKFGFKPLEPEVIVDGTVMDEGQVVSAIKTLFDELNVKVKQVAVSISGHAVIIKK
                     ISLPPMPDDELEGQVKLAAEQYIPFDINEVNIDFHVLPPSEGDGSGEAEMSVILVAAK
                     KDKINELTELVKGAGLLPLVMDVDAFAIENMHAINYPMSQEETTALVNVGASVMNINI
                     VSKGTSLFTRDIPIGGNRYTESIQRELGLSYEEAEQLKKGGKASGNNQSAAAQSVIES
                     VNAEVASEIARTIDYYKSTVAEGDVQQVLLCGGGAQVSGLVKQLRDRMHAVVEVANPF
                     AEVDTSGSDFDQTTLAEMASMASVGVGLALRSVGDR"
     gene            6597..7151
                     /locus_tag="VFR82_03500"
     CDS             6597..7151
                     /locus_tag="VFR82_03500"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEU4500692.1"
                     /translation="MIRINLLSAGPKGRQAKPQYDVRAQVLLGVGLLVITLSACWWYS
                     ASLDDEIATRQTEKSEKEKQVAQLKEQVKQVSDFELRKKQLEDKNRIIDQLEKSRVGP
                     VKVMDHVSHSLEPLKLWLVRVGVAGQNIEVEGRALTNDDVVEFVNNLRRTDYFSDINL
                     QESRAAVESQVSLFQFKLGFRLKG"
     gene            7157..7777
                     /gene="pilO"
                     /locus_tag="VFR82_03505"
     CDS             7157..7777
                     /gene="pilO"
                     /locus_tag="VFR82_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEU4500693.1"
                     /translation="MKLPAMNLEALRTIPFMQKVAFLILVLAGIVVMFYYYVFEPKSM
                     EIAALEGEVGKLDTEIQTLTIKVKHLDELIAASKQLEIELAKKKERLPPEEEAVMLLK
                     QVSDLGIKLGLDIKLWKPGAKSEDPSKLFVRLPVNVEVTGGYHTAALFFDRINGLPRI
                     VTVSNLKMGMPKTEKGRVVTQTVFDLVAYAAPQDVKVAAAPAPAPK"
     gene            7805..8401
                     /locus_tag="VFR82_03510"
     CDS             7805..8401
                     /locus_tag="VFR82_03510"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEU4500694.1"
                     /translation="MIKIGGLGTLFGCMLLLAMVGRSSAEPGLASHLGQVAPLRQDSL
                     KVPSSNDLTKEAPQPALALSPDFAAAPTDALPGAPPFPVGGYDPAGRRDPFSPVLSQL
                     APGQTDPTLPPLQRIGLTDLNLIAIIWGAYGYTAMVQTPDGNGYTVRKGTRVGPNSGV
                     VSAVTEKGIIVQERFTDVYGRKQEREYVKLLHPKEGLE"
     gene            8398..10365
                     /gene="pilQ"
                     /locus_tag="VFR82_03515"
     CDS             8398..10365
                     /gene="pilQ"
                     /locus_tag="VFR82_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEU4500695.1"
                     /translation="MTHIHRHASTTLRILSALTAVSLPVAIPSMGATADVTADVSMSR
                     AGNDQASSSMRSASAVTAIDVKKDADIVSVTISGDGSLSHEALRLGERRLVVDIPRVS
                     SEIHRPTLNVGHRLLKQIRIGDHSDKVRIVFDLGHQAEYAVEPQGASLLVRVSESIGV
                     GSAREQESTILQGIAAQSAAADSPRVEVAQAGAHKAQPVMHRATPAFRVRPIQMMSEP
                     ETVEKHTPKEDLVVGETRYVGRRISLDFQQADISNVLRLIAEVSGFNIVIGDGVKSKV
                     TMKLVSVPWDQALDMILKMNGLGKIKQGNILWIDTLSNIAKQEDEEARAKDAKVKAED
                     LVDRVFYIRNLQATELMTALRQNLSPRGLMQISQGTNALVVRDTETKMAVLKQLIDGL
                     DLEVPQVQIEARIVQADTVYARGLGVQWGIANADFGTNTFSAVGNLTGAFAPAAGTGA
                     TAIPRDFLVNLPAQVAGLPSVPGIGYSFGKLAPGFALDMRLSAGELLGLAKVIAAPKV
                     TTLDKREAKISQGESIPFQTTSLQGTQTTFVDANLELNVTPQITSRDPKEVGKQIMMK
                     VRATRNAVGARSNPAGPSIDRREANTQVIVRDGETMVIGGVFIDTQNNNVQGVPYLSR
                     IPVLGWLFKNKSESVSKQELLIFLTPSIVKA"
     gene            10548..11675
                     /gene="aroB"
                     /locus_tag="VFR82_03520"
     CDS             10548..11675
                     /gene="aroB"
                     /locus_tag="VFR82_03520"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU4500696.1"
                     /translation="MIGQHVPVRLGERSYDIVVEPGLSRCLGALLKPLMETGKIGIVT
                     DRHVARHYLKPTIESLKEAGHEPVTIVLPPGERSKTLTTIARILDVLAAQRFERRSML
                     LALGGGVIGDLTGFAASIYQRGIAYVQVPTTLVAQVDSSVGGKTGVDHRLGKNLIGAF
                     HQPRAVFIDPFTLLTLPRREWIAGLAEVIKYGIIADEVFFTRLEASMPDLLKREAHAV
                     IQAVTRSCQIKAEVVAADERESDRRRILNFGHTIGHALEVLCNYRNLIHGEAVAIGMV
                     AEADLSVHLGLCGPDVAARIKRLVQAAGLPDHMPKTSFAALWGAMQHDKKVVGGKVMG
                     VWPVRLGDVVMRPLEAEACADWHATLTRRASARRKRGIRRA"
     gene            11678..12940
                     /locus_tag="VFR82_03525"
     CDS             11678..12940
                     /locus_tag="VFR82_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEU4500697.1"
                     /translation="MAKRPPSIMQLEQALREKTREVDVLHRITDSISNTLDLEAVLKH
                     IVEIVVEVTKADACLLYLLSDGEDELILRASKNPHPKLIGRITIGMGEGITGWVARER
                     TRVVIPSNASDDPRFKFFHNLPEDRHQAFVSVPIMAKKEVVGVINVQHKRPKRYRPDE
                     LALLSTIANQVGGAIENARLYEQMRRKALQVETLSQVSETVASNRLIEDVMQLLVTMT
                     AQMMNSKICSIMLLDETTGDLRIEATQSLSEHYRRKPNLKVGQSVSGRAVQERRPIMV
                     SDVTKEQDYMYPDMAKKEGLCSLLSVPMLVREKAVGVINSYTSVPHTFTSEEVKLLQA
                     IANQAAIAIEHTTLLEKSFEMQEALAVRKLLERAKGYLMRAKKLTEEEAFKLIQRQSM
                     DLRKSMREIAEAVLLAGELEERAEKHRG"
     gene            complement(12941..13840)
                     /locus_tag="VFR82_03530"
     CDS             complement(12941..13840)
                     /locus_tag="VFR82_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500698.1"
                     /translation="MRLFLLGLHLFGGCVLALSGCATGPDVDETINDSPRGSVYLERI
                     ADRSFRAAHPIRLDVVTIARILRGVYVRNDQSTLQTFFSGKPDALRAFSDEEVTFLTP
                     LIAEGLRRAAPDQQVGFRIAQIGLPGHAHNTGGGVGSSEPPLTLHPRESTTGVIHAYG
                     RSLYLTLTEYRLRAEPANTINMANRRLPDNTGLINHTVLFVPEAAKRPDSYLDARSTD
                     KTLVIDYELLAALPASTGSPPSEPSPPPTAAPPPATKERGEPAKNENEIEALRKELQE
                     IKRQLAEQEAERARSPNKSVTPK"
     gene            14099..15484
                     /locus_tag="VFR82_03535"
     CDS             14099..15484
                     /locus_tag="VFR82_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500699.1"
                     /translation="MHEMKRFVVQVGSIALCLVAFGMGGQTAMAVEEQGAGEAAGAGR
                     LSRETETPPAVICGGCVSPTYTSQGKGSITPYGRIELDAIYSNRNTNPLDPGQFNGYA
                     TAAGKSSNSSATFNPRYSVFGLRADRTDGKHVLTGVVETDFYSQTDGAGNISPRLRLA
                     YARYSPNNNKTSVTAGMDWTPVMGLHPNLIDFSIMGYNGNLWQRLPQVTVRHQFTEHF
                     DGLVTAFRFERGLSAIQPQTQRRTFDGFGVGGVPPNGCANGFACSENAFNDPVQMPYF
                     GTRFGYNGTGNMQGFMIALNGAYRYYRSAPTGNVLSIPSGQNINSYLIGTELVIPITK
                     QLKFSGELAYGQALGVEFFRFGQDRNILTGSEVRTLVGWGELDYAHDRDTTFIAGYGF
                     DNPSNSDLRGTVNVPDQQYLLNHRSYLTAVRHIWGDLFLSFEWNHLMTKWTTGESFAG
                     DNFMISTWYNF"
     gene            15548..16906
                     /gene="urtA"
                     /locus_tag="VFR82_03540"
     CDS             15548..16906
                     /gene="urtA"
                     /locus_tag="VFR82_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HEU4500700.1"
                     /translation="MDEMKKDDRSTVPDDSTKDQSRRDFLVQGARMTAGVGIAALLGN
                     LGHWALSYAADKEPIKIGVLHSLSGTMAISEVSLRDVVLMAVDEINAKGGILGRQVKP
                     VVVDPASNWDLFAEKAKQLLLQDKVAVVFGCWTSVSRKSVLPVFEKNNGLLFYPVQYE
                     GEECSHNVFYTGAAVNQQAVPAVEYLMSKEGGAYKKFYLLGTDYVYPRTTNKILRAML
                     LAKKVSEANIMEEYTPFHHQDYQTIVGKIKKFAAGGGGAVISTINGDSNVPFYKEFAN
                     QGLRAEDAPIMAFSVAEDELRGMDTTALVGHLAAWNYFQSVDTPQNKKFATDFKAYCK
                     RNNLPDGEKRVTDDPIEAAYFGVHVWKQAVEKAGKTDVDAVRKAVYGQKFLAPGGEIM
                     MDTGNHHTHKPVLIGEILKDGQFKVVSRSKGLVKPEPWSEYTNPEKGCDWINHQGTYQ
                     KK"
     gene            17163..>17527
                     /locus_tag="VFR82_03545"
     CDS             17163..>17527
                     /locus_tag="VFR82_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500701.1"
                     /translation="MATARHPFVLFGDVVILKRFGLPILLALGLSIGAQEANGTVPPP
                     ATSPLEQALSQITSGDEAVRDAALRVVVELGDSTLISRLDDIRANAERPVRLAIKPVM
                     DLLRNRAKLESPDPDSRRSA"
BASE COUNT         3961 a         4721 c         5263 g         3582 t
ORIGIN      
        1 cagcaaggac gtggtctcca agctttttag cgatgtggcc ggacggttca aggatcgtcc
       61 cggcggatac accagaatgg taaagaccag gcggcggatc ggcgatgcgg ctgaactggt
      121 tgcgatcgaa ctcgtggcac gcgccgaagt ttcgacctca aaaaagcccg ggtcgactcc
      181 cgccaagaca gacaaaacgg agtaggtctc gccccgagac ctactcgcga cgcgcgggct
      241 tcactgccgt cgtgcgatca atcagaacct cgcctcagcg ctgcatgttt cttcgttgct
      301 aacggcgctc agagtttact tgccatcagc tgaatgctat tatcgtgcca ggcttttctc
      361 ctttgaggaa ccggacggaa acattgtgtg gcagcgctgg gcacaacgga ttttattggg
      421 gcttagcctc gctctggtgt gcttcctggt ctatctcctc atgaccaact ccaccgcgtc
      481 tcctcctccc acggccacag cgccgggcac cattaatcag gccgatgcca tcatttccaa
      541 gttcaccttt acgcagacca aaggggacaa agtgcagtgg caggtcgacg cgcaacaggc
      601 gcgtctctac gaacaggaca agagggcgat tctggacgac gtcgccgtga cgctgttcgg
      661 tgtacagggg agggaaatga cggtgcatgg ggatgaaggg accttgaata ccgagaccaa
      721 gaactttctt cttgcgaacc ggtctgagcc attggtgatt tacacgcaga gcggctacac
      781 catttatacc aatcatctgg cttggacgga tcagacacgg gaaatccgca cgcaggaccc
      841 cgtccatatc atcggccatg gtctggatgt aaaagggcga ggcttgcttg gtcacctcga
      901 caccgaagaa ttcgaagtac ttgaggacgt tcatgtggat gtggctcctg cttcttagct
      961 ggtgcgcgat gagtttgccg acggccggtc aggctgccat gggggcagcc tcgaacggag
     1021 cgtcagacga agcgctcgga accaccgtca tctctcagag gatgactttt aagaaccaac
     1081 aaagccaggc cgtattcgat ggtgccgtcg tgctcacgcg cggcacgctc atcgtctatt
     1141 ccgaccggat ggtggtgtcc ttccgaaacc aagaggcctc ggattccaag aaagcgagcg
     1201 aaccgccgaa ggcgggaacc gcaccagcca aaggtccgga cacggtgtcg aatcgggcag
     1261 tcagccaaat tgaagccacg ggccgcgtca aaatcgagcg ggatgcaggc aacgccacat
     1321 gccaaaaagc cgtctattac cgcgacgaag acaagatcgt tctgaccggt gatcccgtcg
     1381 catgggacaa aggcacccga gtcagcggca agcgcatcac catgttcctg gcggaagatc
     1441 gaacggtcgt ggaaggcggc tcacacgtaa gaatcgagcc ggagggtggc accaagtgac
     1501 ggaagcggtg cgtctcgcgc cggaaaccga tggcgttgcc cgttccagcg agccgcgtgc
     1561 ggtgttgcgc gccgatggcc tggtgaaaag tttccgggcg agaaaggtcg tgaaaggtgt
     1621 ttcggtggaa gtctctgccg gagaagtcgt aggtttgttg ggtccgaacg gcgccggcaa
     1681 gacaaccatt ttcgatatga tcgtcgggtt gacccagccg gacgaaggct caatccgcct
     1741 gaacgctgaa gagatcaccg ccttaccgat gtacaagcgt gcgagaaacg gcatcggcta
     1801 tctgcctcag gagtcttcgg tatttcgtcg actgtctgtg caagacaata ttctggcaat
     1861 tctggaaatg ctggattact ctcgatctga gcgggcggaa cgcgtcgatg tcttgctcgg
     1921 tgagctcgat ctggggcaca tcaggacgag catggcctac gcgctgtccg gtggagaacg
     1981 ccgccggttg gaaattaccc gtgccttggc taccaatccc acattcatgt tgctcgacga
     2041 gccgttcgcc ggcatcgatc cgattgccgt tgcggacatc caacagatca tcaccagatt
     2101 gaagggcaaa gggattggta ttctgatcac ggatcataat gtgcaagaga cgttatctat
     2161 taccgaccgt gcatacatca tcaacgaggg ccttgtcctt gaatccggtc ctcctgatgt
     2221 gattgtgaag agcgagacgg cgcgggcggt ctatttggga gaacggttca ctctgtaacg
     2281 ggcgtgttca tccaatgaaa cttcgtcttg atctccgcct cagccagaaa ttaattatga
     2341 cgccgcagtt gcagcaggca atcaagctgt tgcagctgtc acgccttgag ttgcaacaaa
     2401 gcctggctca gcatttgatg gaaaacccct tgctggacga gttgcccgag gaagtggaag
     2461 agaacgaatc aacgacagcc gaggaaaaag cggaagaggc tgcagcctct gcctcgccgg
     2521 ctacggagac ctccgaaacg gatggctcct cgaccgcgga ggaacgggac actgccgacg
     2581 aagtgtcggc ggccgggtgg gaggagtact tcggcagtga ccgcaggaca ggcggcagcg
     2641 aaggccaatc gtcttcatcg gatgattttc cctcctatga acaaaccgtc gccaaagcgg
     2701 cgtcgctcga agaccacttg ctctggcagc tgtccttatc cggcctgtcg gagcgcaata
     2761 aggcgatcgg tcgtttgctg attgggaatt tggacgacga cggctatctg cgcacgcccc
     2821 tttctgagat catcaccgga acggacttca ccgaggcgga agccgagtcc gtgctccgcg
     2881 acatccagac gtttgatccg acgggggtgg gcgcgaggga ccttcccgaa tgtctgctgc
     2941 tgcagctcgg ccatttgggc aaaagcccga tgggatctct cggcgcccgt ccgggggcac
     3001 tcaaaggatc catcgtggag gcggtagtcc tacatcactt gaaggatctc gaaaagaagc
     3061 aatatgccaa agtcgcgaaa gcactcgacg tcaccgtcgc ggaaatcttt caggccacaa
     3121 aggttatcga agcgctcgaa cccaagccag gccgcccctt ttccaatact cagaactacg
     3181 tcatcgtgcc cgatgtgttc gtcgtcaaaa atgaaggaga gtgggtcgtt ctattgaacg
     3241 acgacggatt accccggatg cgcatcagcc cctactacaa acaaatgatg gcctctggcg
     3301 agggcggatc gacagaaacc aaggcgtatc tcgacgaaaa gttgcgggct gcccaatggg
     3361 tgatccggag tatcgaacaa cgcaacaaga ccattgttaa ggtcgtgtcc agcatcgtga
     3421 agttccaaga acaattcttt gaaaaaggca ttcagtacct taagccgctt gtgctcaaac
     3481 aggtagccga agacatcgga atgcacgaat ccacgatcag tcgtgtgacg gccaacaaat
     3541 atatgtactg tccgcaaggc atgctggaac tcaagttttt tttcaacgcc ggcctgcagc
     3601 gggcggatca acccagcgac atgctttcgt cagtgacagt tcgagagatg atcaagaaaa
     3661 tggtggcgga ggaggatgcc aatcatccgc tcaaggacga ggaaatagcc gcgcgcttgc
     3721 gtgagcgtca agtcttgatc gccaggcgga cggttgcaaa gtaccgggcg gaagagaata
     3781 tcccgccagc cacccaacgc aaacgctttt tttaggccag tgcaagacct gcggacttct
     3841 ttccattcga aggagtgcag tgcatgaaaa ttaccggacg gcaaatggat gtcacctctg
     3901 ccctgaaacg gtatatcagg tcccgattcg aacgcctgga acgatacgat gtctcaccgg
     3961 accgtttgga gatcgtactg agcgtgacca agcatcagca tacggcggaa gtggtgtgct
     4021 cgatcaaagg aaagcggttc caggcgaaga cgtcgacgac agagatgtat gccacgatcg
     4081 atcaactggt caaccgcctc gacaaacaga tccgaaagta caaagagcgc cggattgcac
     4141 acaagggaag gaaaaagacg cagctgcccg ctcctgtcgg agcgcccgct gatctgaacg
     4201 acctgaaaat cgtgcgaccc aagattgccg tgctgtcgcg caaagaggct cgtgatcgct
     4261 tggacgatga tccgggcgcc atggtgttgt ttacgtgcgt ggattctgga aagctgcaaa
     4321 ttcttcggcg atctgagacc ggtcacgtcg tgctcattga tccctgaagg ggtcgagatg
     4381 ccaggactca agctcgtggt tatcaccggt ctttctggag cggggaagtc gcacgcgctg
     4441 aaatgcttcg aggacgtcgg gtacttctgt gttgataacc ttcctccagc gttgcttccc
     4501 accttcgtgg aactctgtca tcagcagggc ggcgagatta aaaatgtcgc gcttggcatc
     4561 gacatccggg agcgggtgtt ctttgccgac ttgaccggca tcctgcaacg catcaaggac
     4621 ctcgggcact ccgtcgagtt attgtttctg gaagcccgtg atcccgtgct cgtccgtcgc
     4681 ttctcggagt cccgcaggcc gcacccgttg ttgcccgagc tgcctgtcct ggaaggcgtg
     4741 cggttcgaaa aagagcggtt ggcggaaatt cgggcacacg ccgatcgcat catcgacacc
     4801 tcgaatctca gcgtccatga tttgcgcgat ttactgagcc ggcatttcag ccaggaacta
     4861 tccgcccgac gcctgaccat ctcgttgatc acattcggtt acaagtttgg cgtgccgtac
     4921 gacatcgatt tactgttcga cgtccggttc ttgcgcaacc cctatttcgt ccccgatctg
     4981 aagccgctca cgggcgagga cccccgggtt cgcagttatg tgctcgatga tccggatgcc
     5041 aagggcttcc tggatcggct ggagcagttt tttacctatc tgatcccgct ctttgagcgc
     5101 gagcgtcgca gctatctgaa cgtcgggata ggctgcacgg gcggtcgcca ccgttcagtc
     5161 gtgatcgcca agcggctgca agagagtttc tcttcggccg ggcatcacgt cactctctcc
     5221 caccgagatc tcaccaagcc atagatcgtt cttctctgtt ctttatgcct ccgagcgtcg
     5281 tcgttcgtcc ctttgcccgc tttcttgaca gcgaggacac atggactata cttagggggc
     5341 ggcgtaagaa tcatcaatgt tgaagccact ttggtgcgtg ttgcctcttt gccatcgcct
     5401 gaaaagtgat gcctccatcg gatgcgtgtt ggaaaaatgc aaaaagattt gcgctgagga
     5461 agaagtcatg tacagtttga ggcacatatg ctgaattcac tgaaaagcct cgtcgataag
     5521 gacattttct cactgctgag tcccaagcgg cagttggtcg gcttggacat cggatccagc
     5581 ggcatcaagc tcgttcagct caaggagaac cgtgggcgct atgtgttgca gaagttcggc
     5641 ttcaaaccct tggagcctga ggtcatcgtt gacggcacgg tcatggatga aggccaggtc
     5701 gtctcggcca tcaagactct gtttgatgaa ctcaacgtca aggtcaagca ggtggcggtt
     5761 tcgatttcgg gccatgccgt catcatcaag aagatcagtt tgcctcccat gccggacgac
     5821 gagctggaag gccaggtcaa actcgccgca gagcaataca ttcctttcga cattaatgaa
     5881 gtcaatatcg attttcacgt gcttccgccg tcggaaggtg acggatcggg tgaagcggaa
     5941 atgtcggtca tccttgtggc tgccaaaaaa gacaagatca acgagttgac ggagctcgtc
     6001 aagggtgcgg gactcctgcc gttggtcatg gatgtcgacg catttgcgat cgaaaacatg
     6061 catgcgatca attatccgat gtcccaggaa gagacgaccg ctctcgtgaa tgtcggcgcc
     6121 agcgtcatga atatcaatat cgtctcgaaa gggacgtccc tcttcacacg cgacattcca
     6181 atcggcggta accgctacac ggaatccatc cagcgggagc tggggctctc ctatgaagag
     6241 gcggaacaac tgaaaaaagg cgggaaagcc tctggaaata accagtcggc cgccgctcaa
     6301 agcgtgatcg agagcgtgaa tgccgaagtg gcctctgaaa tcgcgcgcac gatcgactat
     6361 tacaagtcga cggtggctga aggtgacgta caacaagtgc tcctctgcgg aggaggagcg
     6421 caggtctccg ggctggtgaa gcaattgcga gatcgaatgc acgcggttgt cgaggtcgcc
     6481 aatccgttcg cggaagttga cacgtcgggc agcgattttg accagacgac gctcgccgag
     6541 atggcttcga tggcctccgt gggggttgga ttggcgctca gatcggtagg cgatcgatga
     6601 ttcgcatcaa tctactgtcg gcaggaccaa agggacgtca ggcaaagccg caatacgacg
     6661 tgcgggctca agtgcttctg ggcgtcgggt tgctggtcat caccttgagt gcatgctggt
     6721 ggtattcggc atccctcgat gatgagattg cgacgcgcca gacggaaaaa tccgaaaaag
     6781 agaagcaggt ggcgcagcta aaggaacagg tcaaacaggt gtcggacttc gagctgcgaa
     6841 aaaagcaact tgaagacaag aaccgcatta tcgatcaact cgagaagtcg cgcgtgggtc
     6901 ctgtcaaagt catggatcat gtcagtcaca gcttggagcc tctgaaactc tggctcgtga
     6961 gggtaggtgt ggcgggacaa aacatcgagg tggaagggcg cgcgttaacc aacgacgatg
     7021 tggtcgaatt cgtcaataat ctgcgccgca ccgattattt ttcggacatc aatcttcagg
     7081 aaagccgggc tgctgtcgag agccaggtca gcctgttcca gttcaagttg ggatttcgct
     7141 tgaaaggata gcgagcatga agttgccggc gatgaatctc gaggcccttc gaacaattcc
     7201 gttcatgcaa aaggtcgcct ttctgattct cgtgctggcc ggaatcgtcg tgatgtttta
     7261 ctactatgtc tttgagccga agtcgatgga aatcgctgct ctggaaggcg aggtaggcaa
     7321 actggatacc gagattcaaa cgttgaccat caaagttaag catctggatg aactcattgc
     7381 cgccagcaaa cagctggaaa tcgaattagc aaagaaaaaa gagcgcctgc cgccggaaga
     7441 agaagccgtc atgttgttga agcaagtttc ggatctcggc atcaagttgg gtttggacat
     7501 caagctctgg aagccgggcg ccaagagcga ggacccctcc aagttattcg tgcggctgcc
     7561 ggtgaacgtc gaagtgacag ggggctacca cacggcggcg ctgtttttcg accggattaa
     7621 cgggctgccg agaattgtca cggtgtccaa tttgaagatg ggcatgccaa aaacggaaaa
     7681 gggccgcgtg gtcacacaga cggtgttcga tctggtggcc tatgcggcgc ctcaggatgt
     7741 gaaagtggct gcggcacctg cgcccgcgcc aaagtaacga atgctgagga tgcttgtgga
     7801 tcacatgatt aaaattggcg gtctcgggac cttgttcgga tgtatgctcc tgctcgcgat
     7861 ggtgggacgg tcatcggctg agccgggcct cgcatcccac ctcggacaag tcgctccact
     7921 cagacaagat tccttgaaag tgccctcttc taatgacctg acgaaggagg cgccgcagcc
     7981 agccttggcg ctgtcacctg atttcgctgc cgccccgacg gacgcgttgc cgggcgcgcc
     8041 gccctttcca gtcggaggct acgatccggc cggacgtcgg gatccgtttt cgccagtcct
     8101 cagccagcta gctccaggtc aaaccgatcc cacattgccg cccttacagc gcattgggct
     8161 gacggatttg aatctcattg cgattatctg gggcgcatac ggatacaccg cgatggtgca
     8221 gacacccgat gggaacggtt acacggtccg caagggcacc agggtggggc ccaatagcgg
     8281 cgtcgtcagc gcggtcaccg aaaaggggat catcgtgcag gagcggttta ccgacgtcta
     8341 tggcagaaaa caggagcgag agtacgtcaa gcttcttcat ccgaaagagg gcctagaatg
     8401 acacacatac acagacacgc gtcgacgacg ttgcgaattc tttctgcctt gaccgcggtc
     8461 agtttgcccg tggccatccc ctcgatggga gcgaccgcgg atgtgaccgc cgacgtgtcc
     8521 atgagtcgcg ccggcaacga ccaggcgtcc tcgagcatgc ggtcggcttc cgccgtgaca
     8581 gcgattgacg tcaaaaagga cgccgacatc gtgagcgtga ccatttccgg agacggttca
     8641 ctttcgcacg aagcgctccg attgggtgag cgtcggctgg tcgtcgatat cccgcgagtc
     8701 tcctccgaga tccacagacc cactctgaat gtcgggcacc gcctgctgaa acaaattcgc
     8761 attggagatc acagcgacaa ggtccggatc gtgtttgatc tcggtcacca agcggagtac
     8821 gccgtggagc cccagggagc tagtctgctc gtccgggtga gcgagtccat aggcgtcggg
     8881 agtgcacggg agcaggaaag caccattctt cagggcattg ccgctcagtc cgccgcggcg
     8941 gacagtcctc gtgtcgaggt tgctcaggcc ggcgcacaca aggcacagcc ggtaatgcat
     9001 cgggcaacgc cggcatttcg cgttcgtccg attcaaatga tgtctgaacc ggaaacggtg
     9061 gaaaagcata cgccgaaaga agacctggtc gtgggcgaga cgcgttatgt cggccgtcgg
     9121 atctctttag acttccagca ggcagatatc agcaacgttc tgcggctgat cgcggaggtc
     9181 agcggcttca acattgtgat cggtgacggc gtcaagagca aagtcacgat gaagctcgtg
     9241 agtgtgccct gggaccaagc gttggacatg attctcaaga tgaacggtct tggaaagatc
     9301 aagcagggga acatcctctg gatcgatacg ctgtcgaaca ttgccaagca agaggatgag
     9361 gaggcgagag cgaaggatgc caaggtcaag gcagaagatc tcgtcgatcg cgtcttctat
     9421 atccgcaatc tccaggcaac agagttgatg accgcgctgc ggcagaattt gagcccaagg
     9481 ggtctcatgc agatcagcca aggcaccaac gccctggtag tccgcgatac cgaaaccaag
     9541 atggcggtgc tcaagcagtt gattgatgga ttggatctcg aagtgccgca agttcaaatc
     9601 gaggcgcgca tcgttcaggc cgatacggtc tacgcgcgcg ggctgggcgt gcaatggggt
     9661 attgcgaatg ccgatttcgg aacgaacacg ttctcggcag tgggcaacct cacaggggcg
     9721 tttgctccgg ccgcaggaac gggggcgacg gcgattcccc gggatttcct tgtgaacctg
     9781 ccggcgcagg tggccgggct tccgtcggtg ccgggcatag gctattcgtt cggcaaattg
     9841 gcgcctggtt ttgccctgga catgaggctc tctgccggag agttgctggg gctggccaag
     9901 gtcatcgctg cgccgaaagt tacgacatta gacaagcggg aggccaagat ttcgcagggc
     9961 gaatcgattc cgttccaaac gacttcactt caaggcacgc agaccacctt cgtggatgcc
    10021 aacttggaac tcaacgtcac tcctcagatt acgtcgcgtg atccgaagga ggtcggcaaa
    10081 cagatcatga tgaaagtgcg tgcaactcga aatgcggttg gcgcgcgcag caatccggcc
    10141 ggtccgagca tcgatcgtcg agaggccaac acccaagtga tcgtgcggga tggcgaaacg
    10201 atggtcatcg gcggcgtctt tatcgatacc cagaacaaca acgttcaggg ggtaccgtat
    10261 ctcagccgga ttccggtgtt ggggtggttg ttcaagaaca agtcggaaag cgtatcgaaa
    10321 caagaactgt tgatcttcct gactccttcc atcgtcaagg cctaacgccc cctgcgatcc
    10381 ctgaccaagg ggattgcagg gtcgtgtgat ttctctccgt agctgcctgc cttcctcttt
    10441 gttgataccg ctcctggcag tcttgaaagc tattatcctt caggggcttg tgctactatc
    10501 cccaggttta cttgtacacg acacatccta ccgccacgat gacaagcatg atcggtcaac
    10561 atgtccccgt tcgtctcggc gaacggagct atgacatcgt cgtcgagccg ggcttgagtc
    10621 ggtgcctcgg cgcccttctc aaacctctga tggagaccgg caagatcggc atcgtcaccg
    10681 atcgccatgt cgcgcgtcac tatctcaagc cgacgattga gtcgttgaag gaggccggac
    10741 acgaaccagt cacgatcgtg ctcccccccg gcgagcggtc gaagacgctg acgaccatcg
    10801 ctcgcatctt ggatgtactg gccgcgcagc gattcgagcg ccggtctatg ctgctggcgc
    10861 tcggaggcgg cgtgattggc gatctcacgg gatttgccgc gtccatctac cagcgcggca
    10921 ttgcctatgt gcaagtgccg accaccctgg tggcccaggt ggattcaagc gtcggaggaa
    10981 agacaggcgt cgatcatcgc ctgggaaaga atttgattgg ggccttccat cagccgcgtg
    11041 cagtcttcat cgatccgttc accctgctca cattgccgcg ccgggaatgg attgcgggac
    11101 tggccgaagt gatcaagtac ggtatcatcg ccgatgaagt gtttttcacg cgtcttgaag
    11161 cgtccatgcc cgatctcctc aagcgggagg cgcatgccgt catacaagct gtgacacgct
    11221 cctgccaaat caaggcggaa gtcgtcgcgg cggatgaacg ggagtctgac cggcggcgca
    11281 tcttgaactt tggacacacg atcgggcacg cgctggaagt gctgtgcaat tatcgaaacc
    11341 tcatccacgg cgaagccgtg gccatcggca tggtcgcgga agccgacctc agcgtccatc
    11401 tcggcctctg tgggccggat gtcgcggctc gtatcaagcg cctggtccag gcggcgggtc
    11461 ttcccgatca catgcccaag acgtcatttg ctgctttatg gggcgccatg cagcacgaca
    11521 aaaaagttgt cggcggcaaa gtgatgggcg tgtggccggt tcgtctcggc gacgtcgtga
    11581 tgcgtccgct ggaagcggag gcctgcgccg actggcatgc cacgctgacg agacgcgctt
    11641 ctgctcgacg gaaacgcggg atacgaaggg cttagccgtg gccaaacgtc ccccctcgat
    11701 catgcaactt gagcaagccc tccgggaaaa gacccgcgag gtggacgtgt tgcatcgaat
    11761 caccgattcc atcagcaaca ccctcgatct cgaagcggtc ttgaagcaca tcgtcgagat
    11821 tgtcgtcgag gtcaccaagg ccgacgcctg tttgctctac ctcctttcgg acggtgagga
    11881 cgaactgatc ttgcgcgcct cgaaaaatcc acatcccaaa ctcatcgggc ggatcaccat
    11941 cggcatgggt gagggcatca cgggctgggt ggcgcgagag cggacgcgag tcgtgatccc
    12001 gagcaatgcg agtgacgatc cgcggttcaa atttttccac aatttacccg aagaccggca
    12061 ccaagccttc gtgtccgtcc cgatcatggc taagaaggaa gttgtgggcg tgatcaatgt
    12121 gcagcacaaa cgcccgaagc ggtatcggcc ggacgaattg gccttgctgt cgaccattgc
    12181 caatcaggtg ggaggcgcga tcgaaaacgc gcgattgtac gaacagatga ggcgcaaagc
    12241 cttgcaagtc gaaacactgt cccaggtgtc ggaaacggtg gcatcgaacc gcctgatcga
    12301 ggacgtgatg cagttgcttg tgacgatgac ggcgcaaatg atgaactcga aaatctgttc
    12361 gatcatgttg ttggacgaga cgacaggaga tctgcggatc gaggcaacac aaagcctcag
    12421 cgaacattac cgacggaagc cgaacctgaa agtggggcaa agtgtgagcg gccgggctgt
    12481 gcaggaacgc cggcccatca tggtcagcga cgtcacgaag gaacaggact acatgtatcc
    12541 ggatatggca aaaaaggaag ggctctgctc gctcttgtcc gtgccaatgc tggttcgcga
    12601 aaaagcggtc ggcgtgatca acagctacac gtccgtgccg catactttta cgagcgagga
    12661 agtgaagctc ttacaggcga tcgccaacca ggcggccatt gccatcgagc acacgaccct
    12721 gttggaaaag tcgttcgaga tgcaggaagc actggcggtc cggaagctgc tcgaacgcgc
    12781 caaaggctat ctgatgcgag ccaagaagct gacggaagag gaagcgttca agttgatcca
    12841 gcggcaaagc atggacttgc gaaaatccat gagggaaata gccgaggcgg tgctgttggc
    12901 cggggaactc gaagaacggg ctgaaaaaca cagagggtag tcactttggc gtcacggatt
    12961 tgttcggcga gcgagcgcgc tcagcttcct gctccgccaa ttgccgtttg atctcctgca
    13021 attccttccg gagggcttcg atctcgttct cgttcttcgc cggttctcct ctttcctttg
    13081 tcgcaggcgg cggtgcggcc gtcggcggtg gtgacggctc cgagggcggc gatccggtcg
    13141 aggccggcaa agcggccaat agctcgtaat cgatgacgag cgttttgtcg gtggagcggg
    13201 catccagata gctgtcgggc cgtttggcgg cttcgggcac gaacaagacg gtatggttga
    13261 tcagacccgt gttgtccgga agtcggcggt tggccatgtt gatggtgttg gccggttcag
    13321 cgcgaagccg gtattccgtg agggtcaaat acagggatcg tccgtaagcg tggatgacac
    13381 cggttgtcga ttcacgggga tgaagggtca gcggtggttc tgatgagcca acgccacctc
    13441 ccgtgttatg cgcatggcca ggaaggccga tttgcgcgat tcggaatcca acctgctgat
    13501 caggggccgc gcgcctaagg ccttctgcga tgagcggggt gaggaaggtc acctcctcgt
    13561 cggaaaacgc tcgaagcgca tcgggttttc ctgagaaaaa ggtttgcagg gtgctttgat
    13621 cgtttcgaac gtagacacct cttaagatcc gggcgatcgt cacgacatcg agtcttatcg
    13681 ggtgggcagc acggaaagat cggtcggcaa tccgttcgag atacacggat ccgcgtgggg
    13741 agtcattgat cgtttcgtcg acgtcgggtc cggttgcaca accggagagg gccagaacac
    13801 agccgccaaa caggtgtaag ccaagcagaa agagccgcat tctcctccgt caattactgg
    13861 agcgcgcctc agtctagcac aggtttcaag aagcttgaca gcgcgagcgg gaacacttat
    13921 aagtagcgcc cattaaatcg ggctgaatac ggacaaggaa gtccgatttt cagtcggcat
    13981 atgtcaaagg acaatggcgt ccttttggtc catgatgcgg accggaaggg cgtttttttt
    14041 tgaacgctgc ctccgagatc catctggtat tgagaagtta acgaagaggg agacagccat
    14101 gcacgagatg aaaagattcg tggttcaggt cggaagcatc gcgctctgcc ttgtggcgtt
    14161 tggtatgggt ggacagacgg cgatggctgt ggaagaacag ggagccggtg aggcagcggg
    14221 agccggccgc ctgtcgcggg agacggaaac tccgccggcg gtgatctgcg gaggctgtgt
    14281 gagtcccacc tatacgtcac aaggcaaagg cagtatcacc ccatacggtc gcatcgagtt
    14341 ggatgccatc tacagcaaca gaaacaccaa tcccctcgac cccggacaat tcaacggcta
    14401 tgccaccgcc gcaggcaaga gcagcaacag ctccgccacg ttcaatccgc gttatagtgt
    14461 cttcggcctc cgcgcagatc gaaccgacgg aaagcacgtg ctgacgggcg tggtggaaac
    14521 agatttctat tcacaaaccg acggtgcggg aaatatttcg ccgcgtcttc gcttggccta
    14581 tgcgagatat tcgccgaata acaacaagac gagtgtgaca gccggtatgg actggacgcc
    14641 ggtcatggga ttgcatccga acctgatcga tttttcgatc atggggtaca acggaaatct
    14701 ctggcagcgt cttccgcaag tgaccgtgcg ccatcagttc accgaacact tcgacggatt
    14761 agtcacggcc ttccgctttg agcgtggcct atcggccatt caaccgcaaa cccagagacg
    14821 gacatttgat gggttcggtg tcggcggtgt tcctcccaat ggttgcgcca atggatttgc
    14881 ctgttccgaa aacgccttta acgacccggt ccagatgccc tatttcggca cccgcttcgg
    14941 gtataacggc accggcaaca tgcaggggtt catgatcgcc ctgaacggcg cataccggta
    15001 ttaccgatcg gcaccgacag gaaatgtcct aagtattcca tccggccaga acatcaactc
    15061 ctatctgatc ggcactgaac tcgtgatacc aatcacgaag cagctgaagt tctcgggaga
    15121 actcgcctat gggcaggcat tgggtgtcga gttcttccgt ttcggccagg atcgaaacat
    15181 cctcaccggc agtgaagtca gaacgttggt gggatggggt gagctggact atgcgcatga
    15241 tcgggatacg acattcatcg cggggtacgg attcgacaac cctagtaatt ccgacttgcg
    15301 aggcacggtg aacgtgccag atcaacaata cctcctcaac caccgttcgt atctcacggc
    15361 cgtccgccac atctggggtg acctgttcct gtcgtttgag tggaaccacc tcatgacgaa
    15421 atggacgaca ggtgagagct ttgccggaga caacttcatg atctcaactt ggtataactt
    15481 ctaacatgca gcagtcagcc ttcagcgaac gctgattgtg aacggctatc tgcgctaagg
    15541 ggacaaaatg gatgaaatga aaaaagacga tcgaagcacg gtacccgacg actctaccaa
    15601 ggaccaatcg cggcgcgact ttctcgtgca gggcgccaga atgacggcgg gagtcggcat
    15661 cgccgcgctt ctgggcaatc tcggtcactg ggcgctgtcc tacgcagcgg acaaggagcc
    15721 gatcaagatc ggcgtcctgc attccttgag cggcaccatg gcgatcagcg aagtgtcgct
    15781 gcgcgatgtg gtcctcatgg cggtcgatga aatcaacgct aaaggcggaa ttttgggccg
    15841 gcaggtgaag ccggtggttg ttgatcccgc ctccaactgg gatctgttcg ccgagaaggc
    15901 gaagcagctc ttgctgcagg acaaggtcgc ggtcgtcttt ggctgttgga catcggtcag
    15961 ccggaagtct gtgctgccgg tgttcgaaaa gaacaacggc ttgctcttct atccggtgca
    16021 gtacgaaggc gaagaatgct ctcacaacgt attctacacc ggtgcggcgg tcaatcagca
    16081 ggcggtgccg gccgtcgagt atttgatgag taaggaaggc ggagcgtaca aaaagtttta
    16141 tcttctcggt actgactatg tgtacccgcg gacgacgaac aagatcctgc gcgcgatgct
    16201 tctggccaaa aaagtgtcgg aggccaacat catggaagaa tacaccccat tccaccatca
    16261 ggattaccag acgatcgtcg ggaaaattaa gaaattcgcg gccggcggcg gaggggcagt
    16321 cattagcacc atcaacggcg acagcaatgt gccgttctac aaggaatttg ccaatcaggg
    16381 cctgcgtgcg gaagatgcgc cgatcatggc gttcagtgtg gccgaagacg agctgcgcgg
    16441 gatggatacc accgccctgg tcgggcatct agccgcctgg aactacttcc agagtgtgga
    16501 cacgccgcag aacaagaagt tcgccaccga tttcaaggcc tattgcaaga ggaacaacct
    16561 gccggatgga gaaaagcgtg tgacggacga tccgatcgag gcggcgtact tcggcgtcca
    16621 tgtctggaag caggcggtcg aaaaggccgg taagacggat gtcgacgccg tgcgcaaggc
    16681 cgtctatggg caaaagttcc ttgcgccggg tggagagatc atgatggata ccggaaacca
    16741 tcatacgcac aagcccgtgt taatcggcga gatcttgaag gacggccaat tcaaggtggt
    16801 ttcccgctca aagggattgg tcaagccgga gccttggagc gagtacacga atcccgaaaa
    16861 aggttgcgat tggattaacc atcagggcac ataccagaag aagtaggcgg tcgagccggg
    16921 tatcctcgca ctcgttcggt gtggagccca cgctgccgcc gcttggtgca agccgcgtct
    16981 cggcgcggca gggtgggtgg gtgaaaatag gtgtaagccg cgtctcagcg cggcggggtg
    17041 ggtgggtgaa acaggtgcaa gccgcgtctc agcgcggctg agtgggtggt gaaatagcgg
    17101 cgagggcaat gccaccccct tactctcttg cagcggcaaa agtgttgaac taggcgggtg
    17161 atgtggcaac agcacgtcac ccgtttgtct tgttcggaga tgtcgtgatc ctcaagcgat
    17221 ttggcttgcc gatcttgttg gcgctgggtc tgtctatcgg tgctcaagag gcgaatggga
    17281 ccgttccgcc acctgccacc tcgccgctcg aacaggcgct tagccagatc accagcggag
    17341 acgaggccgt ccgggatgcg gcgcttcgcg tcgtggtcga actcggcgat agcacattga
    17401 tttcgcgact tgatgacatt cgtgcaaatg ccgagcgccc ggtacggctg gctattaaac
    17461 ctgtgatgga tctgcttagg aatcgagcca aattggaaag tccggatccc gatagtcgtc
    17521 gctcggc
//
LOCUS       DASTQY010000110         4426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_413999, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000110 DASTQY010000000
VERSION     DASTQY010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413999"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFR82_03550"
     CDS             complement(<1..254)
                     /locus_tag="VFR82_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU4500702.1"
                     /translation="MARLQLDKTYDPKAVEARWYQFWTERRYFHAAPSHPGQAYSIVI
                     PPPNVTGSLHVGHALNHSLQDILIRWRRMQGRNTLWLPGT"
     gene            complement(573..1727)
                     /locus_tag="VFR82_03555"
     CDS             complement(573..1727)
                     /locus_tag="VFR82_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4500703.1"
                     /translation="MPSTIFVIDSSPAVRRMVEQISTPEGFDVVGFQDGPAALEAARR
                     MSPTLIITDYHLDNMTFSGFCKEVHKLDNLADTYIIALTGATDKLENTHLKALGVKAF
                     LKKPFQSEHLLDIIKDLDRTNTSKDSGKKKRSWPPTSSALDSDDEFAGDSNDAMSDEE
                     LHPIIPMTLDVTPGKKEAMKPPMPAFTAPTPPAAEPEEAMKGLFGQLLESMSGKTEKK
                     IAEMLPRMVNTGLEAQVTRAVEAEVHQQVGAAVSQERLTEVIEPLVLQALPKILTSEM
                     TLLEPIIRHSMFEIASPLIKEHIEQMVREQADAVRQSLQDAVRDHLRSVEEEVRTQLK
                     EAATAETKKLATDVVRTIAEDQIRTTVEMVAPSIVEEQIRAEIARLTKAA"
     gene            1864..2583
                     /locus_tag="VFR82_03560"
     CDS             1864..2583
                     /locus_tag="VFR82_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU4500704.1"
                     /translation="MSQTIQVVLFDAAETLFHVNGSVAEIYLRHAAEFGYPRKADSLQ
                     SITEAFRRAFRDAPPPVFAATESAQIKQSERLWWFDVVHNVFYRVGMFERFDEFFERV
                     FDVFADPSSWKLYPEVLPTLKELRSRGLELGIVSNFDSRLFNVLRGLGIAEYIDTVTI
                     SSLAHAAKPSPKIFELALEKHAVEAADALHVGDSITEDVEGARKAGIRALLLAREEKQ
                     PPPGIAAIRALDELLPLIASR"
     gene            complement(2617..3342)
                     /locus_tag="VFR82_03565"
     CDS             complement(2617..3342)
                     /locus_tag="VFR82_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEU4500705.1"
                     /translation="MASGSDLGLLKRKLESARSLAILTGAGISADSGVPTFRGPDGLW
                     RNFRAEDLATPEAFARDPRLVWEWYNWRRELIATKTPNPAHVAVVEFERRFEEFWLIT
                     QNVDGLHRAAGSEKLSEIHGNIWKVRCTACGIVEDNHDVPIAILPTCRHCQGLLRPHI
                     VWFGESLCSDDLACCAAALRACDVLLVIGTSGVVYPAAGFASVAKETGAFVAEINFEA
                     TPQSGTMDLALKGRAKDLVPLLL"
     gene            3429..>4426
                     /locus_tag="VFR82_03570"
     CDS             3429..>4426
                     /locus_tag="VFR82_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEU4500706.1"
                     /translation="MNNILIVGAGSVGGFFGAHLTKHHPGVSFLLRPKTLAAVQQNGL
                     QVRSASGTVTVHPRAAADARQLPQPDLIMLAVKAYDLDEVLTQIEPVLTERTVILTLQ
                     NGIDTEDRIIERLKRDCVVGGVAYIYSKIVAPGVIDHYKKGSVVIGELMGHESARLLE
                     IRELFASAGLPCQLSKDIRRAKWEKMCWNCVFNPLTVLVDDKVSAALDHPEMMNVIRQ
                     IVHEITAVSAALKVPLPLDMPDRVVKWTQEIRDIHTSMYDDWKAGRQTEVRNLNGYIV
                     ERGHELGIPTPVNEALAAMIKTITEKPRTGPGIIEIDGAVVQPVAFDRMALRQLPA"
BASE COUNT          906 a         1193 c         1356 g          971 t
ORIGIN      
        1 tcggttccgg gaagccatag tgtgttacgt ccctgcatcc ggcgccaacg gatgagaatg
       61 tcttgtaacg aatgattcaa cgcgtggccg acatgcaacg accctgtgac gttcgggggc
      121 ggaatgacga tgctgtaagc ctggccggga tgggagggag cggcgtgaaa gtatcggcgt
      181 tcagtccaga actgatacca tcgcgcttcg acggctttcg gatcgtacgt tttgtcgagt
      241 tggagacggg ccatggtttg cgctggagag aagcgaggca tcttaacatg cgcgtaggga
      301 cccctcaaga taactgacgg cgagggggga tgcgggatcg gggtctgctt gtgctgtcct
      361 tgagaggcag ggaggcttgt gttatatcat cgccgaattg ccgggcatcg acgggccatt
      421 cacgcttgac cgaggagaga ttatggcaac gggacgagag aaggaccgca agaccagaaa
      481 gaagcatcgg aagaacgtca cacggatgaa agcgttggcg aaagggcggc gcgggacgaa
      541 aaaggggaaa aaaggctaga cgggttccgt tgttaggcgg ccttggtgag tctcgcgatt
      601 tcggctcgaa tttgttcttc gacgatcgag ggggctacca tctcgacggt tgtcctgatt
      661 tggtcctctg cgatggtccg gacgacgtcc gtggcaagct tcttcgtttc cgccgtcgca
      721 gcctctttga gttgcgtccg aacctcctct tccacggatc tcaagtggtc gcgtacggcg
      781 tcctgaagtg attgcctgac cgcgtcagcc tgttcccgca ccatctgctc gatgtgctcc
      841 ttgatcaacg ggctggcgat ctcgaacatg ctgtgtcgga tgatcggttc caatagggtc
      901 atttcgctcg tcagaatctt tgggagcgcc tgcaggacca acggttcgat cacctcggtc
      961 agtcgctcct gagatactgc ggcgcccact tgctgatgca cctccgcctc gacggctctt
     1021 gtcacctgag cttcgagccc tgtgttcacc atgcggggca gcatttcggc aattttcttc
     1081 tctgtttttc cggacatcga ttccaacaac tggccaaaga ggcctttcat cgcttcttcg
     1141 ggctccgctg cagggggagt cggggcggtg aaggcgggca tgggcggttt catcgcctcc
     1201 ttcttgccgg gtgtgacgtc gagggtcatc ggaatgatcg gatgcagctc ctcgtcgctc
     1261 atcgcgtcgt tcgaatcgcc tgcaaattca tcatcggaat caagcgcgct cgacgtcggg
     1321 ggccaggaac gtttcttctt tccgctgtct ttcgacgtgt tggtacggtc caggtctttg
     1381 attatgtcca gcagatgttc cgactgaaac ggtttcttga ggaaggcttt gacgccaagc
     1441 gccttgaggt gcgtgttctc cagcttgtcg gtcgcacccg tgagggcgat gatataggtg
     1501 tcggcgagat tgtccaattt atgcacttct ttgcagaacc ccgaaaacgt catgttgtcg
     1561 aggtggtagt cggtgatgat cagcgtagga ctcatcctgc gggcggcttc tagcgccgcc
     1621 ggtccgtcct gaaaacccac cacgtcaaac ccttcagggg tcgagatctg ttcaaccata
     1681 cgacggacag ccggactgct gtcgatgacg aagatcgttg aaggcactta agtctccaat
     1741 tttattgagc tagttcgagg ctagcagggg gggtattctt tgtcaagaaa acggctaata
     1801 gatgcgccac gccatttgag gtattctccg tagcagtctt cacacaaccc aggaaccatc
     1861 cggatgagcc agacgattca ggtggtgttg ttcgatgcag ccgagacgct gtttcatgtg
     1921 aacggatcgg tggccgaaat ctatctccgg catgccgcgg aattcggcta tccgcgaaag
     1981 gcagactcgc tgcagtcgat cacagaggcg ttcaggcggg cgtttcgcga tgcgcctccg
     2041 ccggtgtttg cagcgaccga atcggcgcag attaaacaga gcgagcggct ctggtggttc
     2101 gacgtcgtgc acaatgtgtt ctaccgtgtc gggatgttcg agcggttcga tgagtttttc
     2161 gagcgggtgt ttgacgtctt tgccgatccg tcttcatgga aactctatcc cgaagtcctg
     2221 ccgacgctga aggaactccg gagtcgtggt ctggagctgg ggatagtttc aaactttgat
     2281 tcacgtttgt tcaacgtcct gcgagggctc gggattgccg agtatattga cacggtgacc
     2341 atttccagct tggcccatgc agccaagccg tcccccaaaa tttttgagct tgccttggag
     2401 aagcatgccg tcgaagcggc cgatgcgctg catgtcgggg acagcatcac ggaagatgta
     2461 gaaggcgcaa ggaaggccgg tattcgggcg ctgctgctcg cgcgtgaaga aaagcagccg
     2521 ccaccaggga ttgcagccat acgtgcgctc gatgaactgc tcccactgat cgcctcccgt
     2581 taagcagtga agggcgggaa gaaggactag ggcctgtcag agcagcaagg gaaccagatc
     2641 cttcgcccgc cccttcagtg caagatccat ggtcccggat tggggtgtcg cctcgaaatt
     2701 gatctcggcg acgaatgcgc cggtttcctt cgcgaccgag gcgaatccgg ccgccggata
     2761 cacgacgccg gacgtgccga tgacgagcag cacatcgcag gcccgaagag ctgcagcgca
     2821 gcaagccaag tcgtcagagc ataacgattc cccgaaccac acgatatgcg ggcgaagaag
     2881 accttggcaa tgacggcagg ttggaaggat cgcgataggg acgtcgtgat tatcttctac
     2941 gattccacag gcggtacagc ggaccttcca aatgttgcca tgaatctcgg atagtttttc
     3001 cgagcccgcc gcgcgatgaa gaccgtcgac attttgcgta atcaaccaga actcctcgaa
     3061 gcgccgctcg aattccacga ctgcgacatg ggctgggttc ggggttttag tggcgatcaa
     3121 ctcgcgccgc caattgtacc attcccatac gagccgcgga tcgcgtgcga atgcttcagg
     3181 ggtggcgagg tcttccgcgc gaaagttgcg ccacaggccg tcgggacccc ggaacgtcgg
     3241 gacaccgctg tccgctgaaa tgccggcgcc ggtcaaaatc gccagggatc gcgccgactc
     3301 gagtttgcgt ttgagcagac ccagatcact gccgctggcc atcgcctcat cacctgtgtc
     3361 cggtggaggc cgcatgctat agtaggcgca ccaatttccg caatgtattg agccttggag
     3421 tcgtatcgat gaacaacatt ctgatcgtgg gagccggttc agtcggcggg tttttcggcg
     3481 cgcatttgac gaagcatcat cctggcgtct cttttctgct gcgtccgaaa acgctggcgg
     3541 ctgtccagca gaatgggctt caagtccgta gcgcctccgg cacggtgacc gttcatccgc
     3601 gggccgcagc agacgcgcgg cagctccctc aaccggattt gatcatgctg gcagtcaaag
     3661 cctacgacct cgacgaagtg ctgacacaga tcgagccggt gctcacggag cgaaccgtca
     3721 ttttgacctt gcaaaacggt attgatacgg aagaccggat cattgagcgg ttgaagcggg
     3781 attgtgtcgt cggcggagtc gcgtacattt actcgaagat cgtcgctccc ggtgtcatcg
     3841 accattataa gaagggaagc gtcgtcattg gtgaactgat ggggcacgaa agcgcacgcc
     3901 tccttgaaat tcgagagcta ttcgcctctg ccggcctccc gtgtcagttg tccaaagata
     3961 tccggcgcgc gaagtgggaa aagatgtgct ggaactgtgt cttcaatccc ctgacggttc
     4021 tggttgacga caaggtgtcc gcggccctgg accatccaga aatgatgaac gtcattagac
     4081 agattgttca tgaaattacg gccgtgtccg ccgcgctcaa ggtgccgctg cccctggaca
     4141 tgccggatcg ggtggtgaag tggacccaag agattcggga cattcacacc tcgatgtacg
     4201 acgattggaa agccggacgg cagactgagg tccggaattt aaacggctac atcgtggagc
     4261 ggggccatga attgggaatt ccgacaccgg tcaacgaagc gttggccgcg atgatcaaga
     4321 caataacgga gaagccgcgg accggtcccg gcatcatcga gatcgacggc gcggtcgttc
     4381 agccggtggc ctttgaccga atggcgctgc gtcagctgcc ggctga
//
LOCUS       DASTQY010000111         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1383017, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000111 DASTQY010000000
VERSION     DASTQY010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383017"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1513)
                     /gene="rseP"
                     /locus_tag="VFR82_03575"
     CDS             complement(116..1513)
                     /gene="rseP"
                     /locus_tag="VFR82_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU4500707.1"
                     /translation="MMASVLAWSPDSVWLLLQKTWWFLVVLGILVAFHELGHFLAARW
                     VGVKVLKFSLGFGPKLLGRQVGETEYLVSAIPLGGYVKLFGEEETEAITPEDRRRSFA
                     HQSLWGKVLIVAAGPGFNFILAYLIFAAWLATGYTLPVPSFKDIAPVLEAVAPDSPAA
                     KAGLMVGDQVKKIDGRDIATRVELLDAAAKGKGAPLTLEVLRDGRIQTLTVTPIQAPA
                     PTQRNDDSGYYLGIEEIPPIVNEVVPGKPAAAAGFREGDRVLSIDGHTIHTWTQMTGL
                     VKDSPGKTLQVDVLRDGHRTSLSVTPLAESATVNGQAVQVGKIGIAGPDRSLMRSANP
                     LMAVYNGLEATWKWTELTVVGLYKMIVGDISSKNIGGPLTIAKISGEAAAQGPANVIF
                     LIALLSINLGVLNLLPIPILDGGHLLFFLIEGILRKPLGERQRELAQQVGLVLLVGVM
                     IFAFWNDLERIFFSH"
     gene            complement(1564..>2672)
                     /locus_tag="VFR82_03580"
     CDS             complement(1564..>2672)
                     /locus_tag="VFR82_03580"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEU4500708.1"
                     /translation="DIVERFPEEFRVVGLTAGNNDEMLAEQIRRFHPLAVAMPTESGA
                     ARLKQRCGGQPVEILSGPEGLAAVASLPEAELVVSAIVGGAGLVPTLAAIRSKKHIAL
                     ANKEPMVMAGQLMQEEARRQGVRIFPVDSEHSAIFQSLEGHRIQDVRRLILTASGGAL
                     WNVPKDQLQEVTPERALQHPNWKMGAKITIDSATLMNKGLEVVEARWLFDIPESRIDV
                     LIHRESIIHSLVEYEDRSMISQLGLPDMRTPISYAMRYPDRLPLDLPSLDLTEIGTLT
                     FCKPDHDRFPCLRLGYESLRVGGTMPAAMNAANEIAVDAFLNGGIRFTDISDIIQSTM
                     QAHAPQAVRSVEVALEADRWAREKADSLVHALAR"
BASE COUNT          562 a          788 c          790 g          532 t
ORIGIN      
        1 ctgcggcgac cttccgaatc agccccgagc gaagcataag ccggtggctg acggtttcgg
       61 cttcacccgg gtcttcccgc agggtgggga gcaggagttg agaagtccgc atatgtcagt
      121 gggagaagaa gatccgttcg aggtcgttcc aaaaggcaaa gatcatgacg cccacgagca
      181 gaaccaatcc aacctgttgc gcaagttcac gctgtcgctc gcccaacggc ttgcgcagga
      241 tgccttcgat caggaagaaa agcaagtgac cgccatcgag gatggggatc ggcagcagat
      301 tcagtacgcc aagattgatg ctgaggagcg cgatcaaaaa aattacattg gccggacctt
      361 gggcggcggc ttctccagag attttcgcga tggtcaatgg gccaccgatg tttttgctcg
      421 agatatcgcc aacgatcatc ttgtacaacc cgacgaccgt gagttccgtc cacttccatg
      481 tggcttccag gccgttatag acagccatca gcggattcgc cgatcgcatg agcgatcgat
      541 ccgggccggc aatgccgatc ttgccgactt gaaccgcctg accgttgacc gtcgcgcttt
      601 cagcgagagg cgtcaccgac aatgatgtcc ggtggccgtc gcgcaagaca tccacctgaa
      661 gcgttttgcc cgggctgtct ttgacgagac ccgtcatctg cgtccaagtg tggatcgtgt
      721 gaccatcaat cgaaagtaca cgatctcctt ccctgaatcc ggcagcagca gcaggtttgc
      781 ctggtacgac ttcattgacg atcggtggga tctcttcgat acccaggtaa tagcctgagt
      841 cgtcgttccg ttgagtcggc gccggcgcct gaatcggcgt gaccgtgagg gtctggatac
      901 ggccgtctcg aagaacctcg agtgtcagcg gcgcgccttt acctttggca gcggcatcga
      961 gcagttccac gcgcgtggca atgtcccgcc cgtcgatctt cttgacttgg tcgccgacca
     1021 tgaggccggc cttggctgcc ggcgagtcgg gcgccacggc ttccagaact ggagcgatgt
     1081 ccttaaatga ggggacgggc aaggtgtaac cggttgccag ccaagcggca aagatcaagt
     1141 aggctaagat gaaattgaat cccggccctg cggcgacaat caggactttc ccccaaagac
     1201 tctggtgggc aaaagaccga cggcgatcct ccggcgtgat ggcttccgtc tcttcctcgc
     1261 cgaaaagctt gacgtagccg cccaacggaa ttgcggacac gagatactcg gtctcaccaa
     1321 cctggcggcc gagcagcttc ggcccaaatc ccaaggagaa cttcaggacc ttgacgccca
     1381 cccatcgcgc agccagaaaa tggccgagtt catgaaacgc gacgagaatg ccgaggacca
     1441 cgaggaacca ccaggtcttc tgcaagagaa gccagacact atcgggagac caagctaaaa
     1501 ccgatgccat catgttcata ctcgatggtt gggggttagc aaaatgccgt tcttcgttcc
     1561 ctgtcagcgg gccagtgcat ggaccagtga gtcggccttc tcccgagccc accgatcagc
     1621 ctccagcgcc acctctactg aacggacggc ttgcggggca tgcgcctgca tggtgctctg
     1681 aatgatatca gaaatatcgg tgaaacggat gccaccattc aaaaatgcat cgaccgcgat
     1741 ttcattggcg gcgttcatcg cggccggcat cgtgccgccg acacggagag actcataccc
     1801 cagccgcagg caggggaagc ggtcgtggtc gggcttgcaa aacgtcagcg tgccgatttc
     1861 ggtcagatcc aaggacggca agtcgagcgg aagccggtcg ggataccgca tggcatagga
     1921 gatcggtgtc cgcatgtcgg gtaagcccaa ttgtgaaatc attgaacggt cttcgtattc
     1981 taccagagag tggatgatgc tttcgcggtg gatgaggacg tcgatccgcg attctgggat
     2041 gtcgaacaac cagcgggctt ccaccacctc cagacctttg ttcatcaagg tggcggagtc
     2101 gatggtgatt ttggcaccca tcttccaatt cggatgctgc aatgcccgct ccggcgtcac
     2161 ctcctggagc tggtctttcg gcacgttcca cagcgcccca cccgaggcgg tcaaaatcag
     2221 acggcgaacg tcttggatgc gatggccctc caaggattga aaaattgcgc tgtgctcgct
     2281 gtcgacgggg aaaattctga cgccttgacg cctggcttct tcctgcatca actggccggc
     2341 catcaccatt ggctctttgt tggccagggc aatgtgcttc ttgctgcgga tcgcggccag
     2401 ggtcggaacg aggccggcgc ctccgacaat ggccgatacc accagttcgg cttccggaag
     2461 cgaggccacg gcggccaggc cttccggtcc gctcaggatc tcgacgggct gaccgccgca
     2521 ccgttgttta agccgggccg cgcctgattc ggtcggcatg gccacggcca gtgggtgaaa
     2581 acggcggatc tgctcggcca acatttcgtc attgttcccg gccgtgaggc ccacgacgcg
     2641 gaattcctca gggaatcgct ccacgatgtc ca
//
LOCUS       DASTQY010000112         3655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_666430, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000112 DASTQY010000000
VERSION     DASTQY010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666430"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            375..818
                     /locus_tag="VFR82_03585"
     CDS             375..818
                     /locus_tag="VFR82_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HEU4500709.1"
                     /translation="MSVPVGSEETILPSSAPATSSSVPVQGMVGEGKAWGLCTAVDLQ
                     DCHPDLIRDAEHIRRYVVELCDLIGMQRFGECQVVNFGSGRVAGYSMVQLISTSLISG
                     HFANDTNNAYLDIFSCKGYDPSVVEEFSKEFFRARRSTATVTLRY"
     gene            883..1224
                     /locus_tag="VFR82_03590"
     CDS             883..1224
                     /locus_tag="VFR82_03590"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEU4500710.1"
                     /translation="MTAPTIKDFFKHLPGKFDAEAADDLEAVYQFDLSGSQGGQYVIT
                     IRKGACHVKEGRHDEPHVTLSMASEDCVKVLNGQLSGPAAAMSGRIKITGDIGLAMQL
                     KALFPTIGEQL"
     gene            complement(1274..>3655)
                     /locus_tag="VFR82_03595"
     CDS             complement(1274..>3655)
                     /locus_tag="VFR82_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU4500711.1"
                     /translation="NIVRTDGKRAVYLRVNKQPIANTVEVVDALRAALPKMIGIPPGV
                     KLGISFDQSVYIRQSIGNLVEQALHGSLLAAAVILVFLRNVTSTLIISVAIPLSIMVT
                     FIVLYFTGQTLNVFTLGGLALGIGRLVDDSIVELENIQRHLNTNQRRWDAILEAAREV
                     AMPIFASTVTTVVVFLPIFFVVGIARLLLIPLTLTIAIALFTSFFVSRTVTPALCFKF
                     LKPEQESHRSMPAWFVSLMTWSRERYESLDRGFEDSLRWVLTHRRLFIVGILVIFGAS
                     LALVPRIGTEFLPVSDESQFRIVLRAPVGQRVEKTEQQVAEVERVLRTNIPAHELETI
                     VSSTGVLAQGRSSLFNPNTGPHTSVISVYLASPEHRSRNQVQIMNDVRPKIVKLFPGV
                     AMFFDPGGLVKRVTSFGSQKAVDVEIYGYDFENAREVIRQVEGVMHKIDGLADIEVSR
                     EENYPEVNVVVDREKAALLGISETDIANAVLFSLNGNGQTDPIIYTDPQNGNEYYISA
                     WLAEEHRKDLTDIENIILTARSGEPVLLKNVATLKLNAGPVKIDRKYFQRVIHITANP
                     LNRDLGAIAEDLETAFAKLQLPNGFSIRLAGQIQQQRETFVGLMFATVLALILVYMVM
                     AAQFKSLVDPFIIMFSVPMGFPGVILVLFLTDTTLSTTSMMGIIMMLGIVVSNGVLLV
                     DYTNVLRRKGLPLQEAVIRAARTRLRPILMTSLATVFGLLPMAIGWGTGGETNAPLAR
                     AVVGGLSVSTLLTLFLVPTMYLILEERFPRRATQEPTVFEEIPSEPPLVPANR"
BASE COUNT          841 a         1009 c         1032 g          773 t
ORIGIN      
        1 tcgggccgat cttgatacga cgcagcctca atgcccagtc ggcggcttga gcccccgtct
       61 cgtcgtatgt tggaagatgc actttgggca ggtgtaatgg atgaactggc cgctcgcgac
      121 cagtcgcttg ttcatcggca ccttacacca ggtgcacagg cggtcgggta gttcggacgt
      181 ggaggcagga gcgttaggac ctgttgacat ggcggcattg tccccgagca ccaagaatct
      241 tgtcaaccga ggcaggatga agcggtcgca aaaaaaattt ccctgccgtc atgcagatga
      301 aaaaaatcgt ggtatagttg cacaacattc cagcatgagc tttttcaccc acctacagaa
      361 aggcggactg ccgtatgagc gttccggttg gttcggaaga gacgattttg ccatcgagtg
      421 ccccagcaac ttcttcctcc gtcccggttc agggcatggt gggagagggc aaggcctggg
      481 gattgtgcac cgccgttgac ctccaagatt gccaccctga tttgatccgc gatgccgagc
      541 atatacggcg ctatgtcgta gagctgtgcg acctgatcgg catgcagcga ttcggcgaat
      601 gccaggtcgt caatttcggc tccggtcggg tggctggata ttcaatggtc cagctgatct
      661 cgacgtccct gattagcggc catttcgcaa acgacacgaa caacgcctat ttggatattt
      721 tcagttgcaa gggctatgac ccgagtgtgg tcgaagagtt ttccaaagag ttcttccgag
      781 cacggcggtc gacggccaca gtcacgcttc gttattgatc aaaccaaaag gggggcggtg
      841 gaacccatcg ctcccttaca cactcagcca ccgctgccga tcatgactgc tccaacgatc
      901 aaggattttt tcaagcacct gcccggtaag ttcgatgccg aggccgccga cgatctcgag
      961 gcagtctatc aattcgactt gagcgggtcg caaggcggcc aatatgtgat caccattcgg
     1021 aagggcgcgt gtcatgtcaa ggagggtagg catgacgagc cgcatgtgac gctttcgatg
     1081 gcgagtgagg attgcgtcaa ggtgttgaat ggtcaactca gtgggccggc agcggccatg
     1141 tcgggacgga tcaaaatcac cggtgatatt ggcttggcga tgcaattgaa ggcgctgttt
     1201 cccacgattg gtgagcaact ctagcctgaa gcgagccgtt ctcccactca atcttcccgc
     1261 gagcaccggt tgtctagcgg tttgccggca ccagcggcgg ttctgaggga atttcctcaa
     1321 acacggtcgg ttcctgggtc gcacggcgcg ggaaccgttc ttcgaggatg agatacatcg
     1381 ttggcacaag aaacagcgtc aagagtgtcg acacactgag gcctccgacg accgcccgag
     1441 ccaacggtgc attcgtttcg ccgccggtcc cccagccgat tgccatcggc aacagtccga
     1501 acaccgtcgc aagcgacgtc atcaagatag gacgcaggcg ggtgcgggca gcccgaatga
     1561 ccgcttcctg caggggcaat cccttccgtc gcagcacatt ggtatagtcc accagcaaca
     1621 cgccgttgga caccacaatg ccgagcatca tgatgatccc catcatcgag gtcgtcgaca
     1681 acgtcgtgtc ggtgagaaac agcaccagga tcaccccggg aaatcccatc ggaaccgaaa
     1741 acatgatgat gaaaggatcc accagtgact tgaactgggc ggccatgacc atatacacga
     1801 ggatcagggc gagaacagtg gcgaacatca ggcccacaaa cgtttcacgc tgctgctgaa
     1861 tctgtccggc caatcggata ctgaagccat tgggcaattg cagtttggca aaggcggttt
     1921 cgaggtcctc ggcgatcgcc cccaggtcac ggtttaacgg attggccgtg atgtggatta
     1981 cccgttgaaa atacttgcga tcgattttca ccggtccggc gttcaatttc agcgtcgcga
     2041 cgttcttcag cagaaccggt tcgccgcttc tggccgtcaa gataatgttc tcgatgtcgg
     2101 tcagatcctt ccggtgttct tctgcaagcc aggcgctaat gtagtactca ttcccatttt
     2161 gaggatcggt atagatgatc ggatccgtct gcccgttgcc gttgagtgaa aacaggacgg
     2221 cgttggcgat gtcggtttcg ctgattccca gcagggcggc cttctcacga tctaccacga
     2281 cgttgacctc cgggtagttc tcctcccgac tgacttcgat atcggccaag ccgtctatct
     2341 tgtgcatgac accctcgact tggcgaatga cctctcgggc gttttcgaag tcatagccgt
     2401 agatctcaac gtcaacggct ttctgtgagc cgaaactggt cacgcgcttg acgagcccac
     2461 ccggatcgaa aaacattgca acgccgggaa acaacttgac gattttcggc cgcacgtcgt
     2521 tcatgatctg gacttgattc cgcgatcgat gttccggcga agccaggtac acggaaatga
     2581 ccgaagtatg cggacccgtg ttgggattga acaaggacga ccgaccctga gccagaacac
     2641 cggtgctgga cacgatcgtt tccaactcat gggccgggat attggtacgc aacactcgct
     2701 ccacctcagc tacctgttgc tctgtttttt ccactcgctg gccgactggt gcacgaagca
     2761 caatgcgaaa ctgactctca tccgaaacgg gcaaaaactc agtgccaatc cgcgggacca
     2821 acgcgagcga cgctccgaag atcacaagga taccgacgat gaacaacctt cgatgagtga
     2881 gtacccagcg gagtgagtct tcgaatcccc gatccagcga ttcataccgc tcgcgactcc
     2941 acgtcatgag actgacgaac caggccggca tcgaacggtg cgattcctgt tccggcttca
     3001 ggaatttgaa gcagagcgcc ggcgtgaccg ttcgtgagac gaaaaacgaa gtgaagaggg
     3061 cgatcgcgat tgtgagggtc agaggaatca gcaacagtct ggcgatgccc actacaaaaa
     3121 agatcgggag aaacacgaca accgttgtga ccgttgacgc gaaaatcggc atcgccactt
     3181 cccgggcggc ttccagaatg gcatcccaac gacgttgatt cgtattgaga tgccgttgaa
     3241 tgttttccag ttcgacgatc gagtcgtcga ccaggcggcc gatccccagc gccaatcctc
     3301 ccaaggtgaa aacgttcaac gtttggccag tgaaatagag aacaatgaac gtcaccatga
     3361 tcgagagtgg aatggcaact gagatgatga gcgtgctggt tacattgcgc aggaagacga
     3421 gaataaccgc tgcggccagt agagaaccat gcagggcttg ctcgacgaga ttgccgatcg
     3481 attgacggat gtataccgat tgatcaaagg agatgccgag cttcacgccg ggcggaatgc
     3541 caatcatttt cggcaacgcc gcacgcaggg catcgaccac ttccacggta tttgcgatcg
     3601 gttgcttgtt gacacgaagg tagaccgccc gtttgccgtc tgtccggact atgtt
//
LOCUS       DASTQY010000113         9511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_464739, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000113 DASTQY010000000
VERSION     DASTQY010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9511
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464739"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VFR82_03600"
     CDS             complement(<1..318)
                     /locus_tag="VFR82_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500712.1"
                     /translation="MEAPSGAKLGFPGADDLANMFQFKRDFRADFLGMRSLSVSRALN
                     PELQTFDTWLAHNKHRIPLTNVRLTGGHLKECSVESILDQPERGRQCVFGAPIRALPS
                     VARP"
     gene            complement(579..1307)
                     /locus_tag="VFR82_03605"
     CDS             complement(579..1307)
                     /locus_tag="VFR82_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500713.1"
                     /translation="MSAHRMIGHHLFVPAIALLALSAQAAEPTSTRLEIWGDYRYKIQ
                     ALERPADAKDLACREALRQAVTNSTLYREHTASIVDHLLLRDIAWTLATRHVQDQEIL
                     EQGEQGRTVFCRVKAYLPADESARAIRMQLAGGQVPSEGLDQNRALRLLSVKEESPGT
                     LVIQYQALKRLDWLNTHYQGGLRDSADIMVDFYDAQGLLLVSRRYEARRTITGEDVMS
                     PGAVGVLQVPKPAAAKTYRVWLVK"
     gene            1559..3805
                     /gene="malQ"
                     /locus_tag="VFR82_03610"
     CDS             1559..3805
                     /gene="malQ"
                     /locus_tag="VFR82_03610"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU4500714.1"
                     /translation="MEDTEQTLLAGLAERVGIAAEYHDISGVRHVTADHTKRDILRAM
                     GFQVDSIATLAVAVREWDEAPWRRPCDPVRILHDEEVGAPVACCFELEDGKERSVVVK
                     WELKDESGQLLQQGQVGPGLAATEVKFIDGARRARVELPAPKGLSLGYYDFLIRAEGL
                     VGGSTGTLRLIVAPRQCYVPTALEVGQRYWGLALQLYSLSSGRNWGCGDFTDLNKIVD
                     WAGKSLGAALIGLNPLHAIRNTAPHNTSPYAPYSRLAINELYIDLERLPEFFASEDAQ
                     KQYRAPEFQAKLQALRENRRVDYDAIAAAKRTMLDLAYRQFLKEAYTGVEPNLEPQTA
                     RAKLLERFIQAEGEALELYAIFQALEEERRLIQPKHVSWQEWPKQFLSPGSAAKEYGK
                     RHRKRIRFFQYIQWIASEQLRDIQRTAEAAGMPIGVYNDLALGAERTGAEAWMYQSVL
                     ALNAECGAPPDAFAPEGQNWGLPPINPVALRPSRYELLIQLLRRNMRFGGAIRLDHVM
                     ALCRLFWIPKGKAAAEGTYVHYPFEDLLAIIALESVRSKTIVIGEDLGTVPDWVRDQL
                     SKAGVLSYRVFYFERRGDGSWKAPNEYPQQALAVVTTHDLPTLTGFWSGEDIQLRSGL
                     GAFGDEAARRQAWDERQRDKGRMLAALKQEGLLPDGVSDDAAAVPAMTPALCRAIHTY
                     LSRTPSWLVLANVEDGLEEISQTNLPGTVENHPNWRRKYASPVENLVGDERIHELGTT
                     LRSTRPIS"
     gene            3970..5127
                     /locus_tag="VFR82_03615"
     CDS             3970..5127
                     /locus_tag="VFR82_03615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4500715.1"
                     /translation="MPKQKNWVILTIAAAVFVVIVVLETVAPANIVGAYAFVLPILLV
                     ATLHSRPLMLATVVACVAATYLGLLQPTKPGRFQAAIINRTVVAGVLLIVAYVLMTRE
                     ERKVREETARQALAHQTESLLRANALLVDAKDQLNRSERLAAVGQLVASVAHEVGTPL
                     HSIAWHVQALGEEPSVTPDMKKRITVIDEQLNRVVRIIQDLLSSTRQRKPDPKWMSVN
                     EVITPATVLMEPAFHAKGVTLTSEMAIGIPSVWADAERLHQVLVNLLTNALAATGQPG
                     TVTVHAGARAATAEELEAGQRVAPDMSPMVVTVAVRDTGVGMPQSDVHRAFQPFFTTK
                     EVGKGTGLGLFLSRETVQAHGGTLSLMSEQGRGTTVTISLPGKVFVPEGLA"
     gene            5136..6545
                     /locus_tag="VFR82_03620"
     CDS             5136..6545
                     /locus_tag="VFR82_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU4500716.1"
                     /translation="MQAAKILVVDDDAVARELLAEALKKEGYDVEAFGSGEAAIARGR
                     EGRVDLVLTDIRMGAVDGLTVLREFKRMSQDTAIVVLTAFGSLEGAIEAIKQGAYDYL
                     AKPFKKEDIKLVVQRSLDHCRLLRENTKFREELKSKDEWSPLVGSSPAMLEVYKLVAR
                     VSESKSTVLLQGESGTGKELIARAIHGNSPRRDKPFVPVNCGALPDTLLESEMFGYEK
                     GAFTGAVGTKTGLFESANGGTLFLDEIGELGQALQVKLLRVMQDHEVRRVGGTHSIKV
                     DVRIIAATNRDLEQFVKEGKFRDDLFYRLNVVRITLPSLTERQEDIPMLAHHFLQKCA
                     AGTTTAVRGFHPDTMDLLKRYRWPGNVRELENAIERAVSLSHGPLLTPDDLPAAIRQT
                     PIHADAKPESPEAAEPVCLTLEEVEKRHLVRVLKETKGNKVKAAKILGIDRRTLYRMA
                     ERFGLDLGDEIEGTEKETG"
     gene            complement(6548..6994)
                     /locus_tag="VFR82_03625"
     CDS             complement(6548..6994)
                     /locus_tag="VFR82_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500717.1"
                     /translation="MTLRSTQLLVAVSLLIGVACAGPTIPHDPDVIDVTLPASAGDVR
                     MAVIQVLTDGGYEVDREDDQNLTTPYRKEMSGPWDGLLRWRFGTGRSRIEALVTPTDE
                     QTTRLRLHVQYDGKDGIFTRWEDSPTPLPQSAMNQLRLIKNALHIL"
     gene            complement(6991..7404)
                     /locus_tag="VFR82_03630"
     CDS             complement(6991..7404)
                     /locus_tag="VFR82_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEU4500718.1"
                     /translation="MKRPKSATAGPYQRPGPASATLTGASGIRYVGKEPAIPAVPCTI
                     HAPVDGGVVMADRAYILIHVLPGQTSRVVRALGEIKQIKTIDPCWGKPDIIAVVEVPD
                     QDALTQLVLSRIHTIDGVSQTDTHLVYRLKDTKSK"
     gene            complement(7412..7960)
                     /locus_tag="VFR82_03635"
     CDS             complement(7412..7960)
                     /locus_tag="VFR82_03635"
                     /inference="COORDINATES: protein motif:HMM:NF037406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500719.1"
                     /translation="MAPSQPSTTTTVTSPSPAPPVSVSNASSPEADHLPITKNICQRF
                     HFVARQLRLRGEYRSSVSVEDESDVQDLLHALLRLQFDDISTDEWTPDYTNGASRTTF
                     LLNHDRLAVVAKKTRAGLTMTDLKDQVRADIERYRARGRCTSLLCFIYDPDGRIGNPR
                     GIESELTSTSEQFTVDIVVAPK"
     gene            7842..8138
                     /locus_tag="VFR82_03640"
     CDS             7842..8138
                     /locus_tag="VFR82_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500720.1"
                     /translation="MFLVIGRWSASGDEAFDTETGGAGEGEVTVVVVDGCDGATSRVE
                     YRTGSAGSAGGSTGGEGEGGGAVALDTGTVGIGFVSWVRNNDEMGSMGTRGGID"
     gene            complement(8797..9042)
                     /locus_tag="VFR82_03645"
     CDS             complement(8797..9042)
                     /locus_tag="VFR82_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500721.1"
                     /translation="MTCRRCQGYMAADHFLDLHESGGEWWLQGWRCINCGYVFDPVLE
                     KNRQLHAAAMASAEAQQSVSSEPVVVRGQEDSLDVAA"
BASE COUNT         2000 a         2548 c         2930 g         2033 t
ORIGIN      
        1 gggccgagcg acactcggga gagctcttat gggtgcgccg aagacgcatt gtcggccccg
       61 ctcgggctga tcaaggatcg attccacgct acactctttc aagtggccgc ctgttaatcg
      121 cacgttagtc agcggaatac ggtgcttgtt atgagcgagc catgtgtcga aggtttgcag
      181 ctctggattg agtgctcgac tgacgcttag gctgcgcata ccgagaaaat cagctcgaaa
      241 gtcccgcttg aactgaaaca tattggcgag gtcgtccgca ccgggaaatc ccagcttcgc
      301 accagagggc gcttccatgt gagatgagtt ggtgacgaag ccaatctgcc gatgtaaggg
      361 cgcgctgccg aaagatcggc gcgatgcggt cgatcagttg tcagatgcag ggaagtcgtc
      421 aaccaaccgt ccggtgggct tctggagcct gccaaaaaca ttgccgcagg tatgaagagc
      481 gctttgaggc acgaatcatc tacactggta cggattgcag cgggcgagaa taaagcgagg
      541 cctccatctc ggaggatctg ctgtcagtac gacgggagct atttcaccag ccacacgcgg
      601 taggttttcg cagcggccgg tttcggaacc tgcaggacgc cgacggcgcc cgggctcatg
      661 acgtcctcgc cggtgatcgt tcgcctcgct tcgtagcgac ggctgaccaa gagcaggcct
      721 tgcgcgtcgt aaaagtccac catgatatcg gctgagtcgc gcaagcctcc ctgataatgg
      781 gtattgagcc aatccaatcg tttgagcgcc tgatactgaa tcacgagtgt accggggctc
      841 tcttctttca cgctgagtag gcgcagcgcg cggttttggt ccaatccttc tgacggtacc
      901 tgccccccag ccaattgcat ccggatggca cgggcgctct catctgccgg caggtaggcc
      961 ttgactcgac agaaaaccgt tcgaccctgt tcgccttgtt ccaatatctc ttgatcctgg
     1021 acgtgacgtg ttgcgagcgt ccaggcgatg tcgcgcaaga gcaagtgatc gacgattgat
     1081 gctgtgtgct cgcggtagag ggtcgagttg gtgacggcct ggcggagcgc ctcccggcag
     1141 gcaagatcct tggcatcggc gggtcgctct aaggcttgaa tcttgtagcg atagtctccc
     1201 cagatctcga gacgggtgct ggtcggttcc gctgcttgag cgctgagcgc gagtaatgca
     1261 atcgccggaa caaaaagatg atgaccgatc atgcgatggg ctgacatgtc cgcctcctca
     1321 aataacaggt ggcaaattgt tcaccgcccg gcggttatgc tatcacgcat gcgtcgtggc
     1381 ttccactctc aggacgcagg ccaccgacct ggcagtctcg gacggcgccg acggctcact
     1441 ttgctaagta aacggccatt gatttgtgca gccattctaa tttgcgtgta taaaaatcac
     1501 tcaagacaac aataggatag cttgacttgc gtttcgtctt cacgcagact cccaatttat
     1561 ggaagacacg gaacaaacgc tgctggccgg cctcgctgag cgagtcggca ttgcggcgga
     1621 atatcacgat atcagcggtg tccgccacgt cacggccgat cacaccaagc gcgacatcct
     1681 tcgagcgatg ggttttcagg tcgattcgat tgcgacactc gccgttgcgg tccgtgaatg
     1741 ggatgaggca ccttggcgca ggccctgcga tcccgtccgc atcttgcacg acgaggaagt
     1801 gggcgcaccg gttgcctgtt gtttcgagct tgaagacggg aaggagcgct cggtcgttgt
     1861 gaagtgggag ctgaaagacg aatccggcca gttgttgcag caaggtcagg tcgggccggg
     1921 tctggccgcg actgaagtga agtttattga cggcgcgcgg cgcgcacgag tcgaactgcc
     1981 cgcgcccaaa ggtctgtcgc ttggatacta tgatttcctt attcgtgctg agggcctggt
     2041 cggtggttcg accggcactc tcaggctgat cgtggcaccg cggcaatgtt atgttccgac
     2101 ggcattggag gtgggccagc gttactgggg ccttgccctc caattatact cgctatcctc
     2161 cggacgcaat tggggctgtg gagattttac ggatctgaat aaaattgtcg actgggccgg
     2221 aaaaagcttg ggcgccgcac tcattggcct caacccgctg cacgccatcc gcaacactgc
     2281 gccgcacaac acgagtccct atgcaccgta tagtcggttg gcgatcaacg aattgtatat
     2341 cgaccttgaa cggcttccgg agttcttcgc ctcagaggat gcgcaaaagc agtatcgcgc
     2401 gccggaattt caagccaagc tccaagcgct gcgcgagaac cggcgcgtgg attacgatgc
     2461 tattgcggct gccaagcgca cgatgttgga tctggcctat cggcagttcc tcaaagaggc
     2521 ctataccggc gtggagccga atctcgagcc ccaaacggcc cgggccaaac tgctcgagcg
     2581 atttatccag gctgaaggcg aggcgctcga actgtacgcg atcttccagg cacttgaaga
     2641 ggagcggcgg ttgatccagc cgaagcacgt cagttggcag gagtggccca agcagttttt
     2701 gtcgccgggg tccgccgcaa aagaatacgg gaaacgccat cggaagcgga ttcgcttttt
     2761 ccaatacatc cagtggatcg cctcagaaca acttcgtgac atccagcgca cagccgaggc
     2821 cgcggggatg ccgattggtg tttataacga tctggcgttg ggtgcggaac gtacgggagc
     2881 ggaggcgtgg atgtaccaat cagtgctcgc gctgaatgct gagtgcggcg ctccgccgga
     2941 cgcgtttgcg ccggaagggc agaactgggg gttgccgccc atcaatccgg tcgcgttgcg
     3001 gccgagtcgc tatgagttgc tgattcaact gctgcgacgg aatatgcgct ttggaggtgc
     3061 catccggctc gatcacgtga tggccctgtg ccggctcttc tggattccca agggcaaagc
     3121 tgcagcggag ggcacgtacg tccattatcc cttcgaggat ttgttggcta ttattgctct
     3181 ggaaagtgtt cgatctaaaa caatagtcat cggggaggat ctcggcacgg ttcccgattg
     3241 ggtcagagac caattgtcga aagccggggt gctgtcgtat cgagtcttct atttcgagcg
     3301 gcgcggtgac ggatcctgga aggccccgaa tgagtatccg caacaggcgc tcgcggtcgt
     3361 gacgacgcat gatctgccta cattaactgg attttggtcc ggcgaggaca ttcagctacg
     3421 ctcgggcctg ggcgcattcg gggatgaggc tgctcgtcgt caagcctggg acgaacggca
     3481 acgtgacaaa ggccgcatgt tggccgccct caagcaggag ggactgttgc ccgatggcgt
     3541 cagcgatgat gccgcggccg ttcctgccat gacgccggcg ttgtgtcggg caattcatac
     3601 ctacctatcg cgtacaccgt catggcttgt gcttgcgaac gttgaggacg gcctggagga
     3661 aatttcacag accaacctgc ctggaacggt cgaaaaccat ccgaattggc ggcgaaagta
     3721 cgccagcccc gttgaaaatc tggtcggtga tgaacgaatc cacgagcttg gcacgacctt
     3781 gcgttcgacc cgcccgatca gctaagacgc tgcacaacgg actctgcaat agctgccagc
     3841 tttgtgctcg gtggcacggg actgcccgca cgcctggaaa aggcctgttc cgacagccgg
     3901 agggtgaagc agtacgcgga gcctcttcag gcgcctgtgg gttgcttgag ttcctcttca
     3961 ggttcagaca tgcccaagca gaaaaattgg gtcattctta ccatcgcagc agcagtgttt
     4021 gtcgtcatcg tggtgttgga aaccgtggca ccggcgaata tcgtcggagc ctatgccttc
     4081 gtattgccca ttctgctggt cgccaccctt cacagccgtc ctttaatgtt ggcgacggtc
     4141 gtggcctgcg tcgcggcgac gtacctgggt ctgctgcaac caacgaagcc gggccgcttc
     4201 caggcggcca ttatcaatcg cacggtcgta gccggtgtct tgctgattgt cgcctacgtg
     4261 ttgatgactc gagaagagcg caaagtgcgg gaagaaacag cgcgccaggc cctggcgcat
     4321 caaacagaaa gtctgctgcg ggcgaacgcc ttgttggtcg atgccaagga tcagttgaat
     4381 cgctcggaac ggttggcggc agtcgggcag ttggtcgcat cggtcgccca cgaagtggga
     4441 acgcccttac attccatcgc ctggcacgtc caagcccttg gcgaagaacc cagcgtcaca
     4501 ccggacatga aaaagcggat cacggttatt gatgaacagc tcaatcgtgt cgtgcgcatc
     4561 atccaggacc tcctctcgtc tacgaggcaa cggaaaccgg atccgaaatg gatgtcggtt
     4621 aacgaggtca tcacgccggc cacggttttg atggagccgg cttttcacgc gaaaggcgtc
     4681 acgttgacga gcgaaatggc catcggcatt ccatcggtct gggccgatgc ggaaaggctg
     4741 catcaggtcc tcgttaattt gctgaccaac gcgctggctg caactggaca gccggggacg
     4801 gtcaccgtgc atgcgggcgc gcgtgccgcg actgcggaag aactggaggc cggccagcgg
     4861 gtcgccccgg acatgtcgcc gatggtcgtc acggtcgccg ttcgcgatac gggtgtgggc
     4921 atgccacaat ccgatgttca tcgggcgttc caaccatttt tcacgaccaa ggaggttggc
     4981 aaggggacag gcttgggtct attcttgagc cgggaaaccg tgcaggctca cggcgggaca
     5041 ctctcgttga tgagcgaaca gggacgagga acaacggtga cgattagcct gcccggcaaa
     5101 gtcttcgtgc cggaagggct ggcctagagg atgctatgca ggcagcgaaa atattagttg
     5161 tcgatgatga tgccgtggct cgggagctat tagccgaggc actgaagaaa gaggggtacg
     5221 atgtcgaagc cttcggcagc ggcgaagcgg ccattgccag gggacgtgaa ggccgggtcg
     5281 atttagtgct tacggatatc cgcatgggcg ccgtagatgg attgacggtt ctgcgagaat
     5341 tcaagcgcat gagccaggac accgcgatcg tagtcttgac cgccttcgga tcgttggagg
     5401 gtgcgattga ggcgatcaaa caaggtgcgt acgactacct ggccaagccc tttaagaagg
     5461 aggacattaa gcttgtcgtt caacgcagcc tcgaccactg ccgcctgcta cgggagaata
     5521 caaaattccg tgaagaactc aagagtaagg acgaatggtc gccgctggtc gggagcagcc
     5581 cggcaatgct ggaagtctac aagttggtgg ctcgcgtgtc cgagagcaag agcacggtgt
     5641 tgttgcaagg agagagcggg acagggaaag agttaattgc acgcgccatc cacgggaaca
     5701 gtccgagacg cgacaagcct tttgtcccgg tgaactgcgg agccttgccg gacacgctgt
     5761 tagaatcaga aatgtttggc tacgagaagg gcgcgttcac aggggcggtc gggacgaaga
     5821 ccggactgtt cgaatcggcc aacggcggca cgctgtttct cgacgagatc ggcgagctcg
     5881 gacaggctct gcaagtaaag ttgctgcgcg tcatgcagga tcatgaggtt cgccgggtcg
     5941 gcgggacgca ttccatcaaa gtggacgtgc gaatcattgc tgcgaccaat cgggacttgg
     6001 aacagttcgt caaggaagga aaatttcgag acgacttgtt ttatcgcttg aacgtcgtcc
     6061 ggatcacctt gccgtccttg accgagcggc aggaagacat tcccatgctc gcccatcatt
     6121 ttctgcagaa gtgcgccgcc ggaacgacaa ctgccgtgcg aggttttcat ccggatacca
     6181 tggacctctt gaaacgatat cgatggccgg gaaacgttcg cgagttggag aatgcgatcg
     6241 aacgcgcggt ctcgctcagt cacgggccgc ttttgacgcc ggatgatttg cctgctgcaa
     6301 ttcgacagac tccgattcat gctgacgcca agcctgagtc gcctgaagcc gccgagccgg
     6361 tgtgtcttac actcgaggaa gttgagaagc ggcatttggt gcgcgtgctc aaggaaacca
     6421 aagggaataa ggtcaaagcg gccaagatct tgggtatcga tcgccggacc ctctatcgta
     6481 tggcggaacg ttttggattg gatctcgggg atgaaatcga gggcaccgag aaagagaccg
     6541 ggtagtgtta gagaatgtgc agagcgttct tgatcagccg gagttggttc atcgcactct
     6601 gaggcaaggg cgtcggcgaa tcctcccacc gagtaaagat tccatccttt ccgtcatact
     6661 gcacgtgcag cctgagtctg gttgtttgct cgtcggtggg tgtgaccaaa gcctcgatac
     6721 gacttctacc cgtgccgaag cgccagcgca ataacccatc ccagggtcca ctcatttctt
     6781 tccggtatgg ggttgtcaaa ttctggtcgt cctctcggtc cacttcgtat ccgccatcgg
     6841 tcagcacttg gatgaccgcc atccgaacgt ccccggccga tgcaggcaac gtgacgtcga
     6901 tgacgtcggg atcatggggg atagtcggcc cggcgcaggc cacgccaatg agcaatgaca
     6961 cggcaaccag gagctgagtg ctgcgcaaag tcacttggac ttcgtgtcct ttagccggta
     7021 cacaaggtgc gtatccgttt gtgagactcc gtcgatcgta tggatacggc tcagaaccag
     7081 ttgcgtcaac gcatcttggt cgggtacttc gacgaccgcg atgatatccg gtttgcccca
     7141 gcacggatcg atcgttttga tctgcttaat ctccccgagg gctcgcacga cacggctggt
     7201 ttgtcccggt aagacgtgaa tcaaaatata cgcacggtcg gccatgacca ctccaccgtc
     7261 taccggtgca tggatcgtac aaggcaccgc cggaatggca ggctctttgc caacgtagcg
     7321 gatacctgaa gcacctgtca atgtggcgga agcgggtccc gggcgctgat acggaccggc
     7381 ggtagcggat ttgggtcgct tcatacctgc ctcactttgg tgcgacgaca atgtcgacgg
     7441 taaactgttc actggtgctg gtcagttcgc tttctatccc tcgcgggttg ccaatgcggc
     7501 cgtccgggtc gtagatgaaa cacagcaggg aggtgcatcg accacgagcc cgataccgct
     7561 cgatgtccgc ccgcacctgg tctttcaggt ccgtcatcgt gagtccggct cgtgttttct
     7621 tcgcgacgac tgccagacgg tcgtgattca gaagaaaggt cgtccgggag gctccgttcg
     7681 tatagtctgg cgtccattcg tccgtgctga tgtcgtcaaa ctgcaagcgt aaaagtgcgt
     7741 gaagcaaatc ctgtacatct gattcgtctt cgacgctcac gctcgaccga tattcgcctc
     7801 gcaagcgtag ctgccgcgcg acaaaatgaa agcgttggca gatgtttttg gtgattggca
     7861 gatggtcggc ctcgggcgac gaagcgtttg atactgagac gggtggagcg ggggaaggcg
     7921 aagtcaccgt cgtggtcgtt gatggctgtg acggcgccac ttctcgagtc gaataccgga
     7981 ccggttcagc cggttcagct ggtggcagta caggtggcga aggagaagga ggcggcgcag
     8041 ttgcgcttga cactggaact gtcggcatcg gtttcgtctc ctgggttcga aacaacgatg
     8101 agatgggctc gatgggtact cgtggtggta tcgattgagt gggtgacgcc gcactcacct
     8161 ccacaggcaa tgcgggggca gtagggggag taatggctgc agcgacagcc gctgactgat
     8221 ctgttgccgt gcttcgtgtc acgtgcaggg ttactggagg cgatgatgtc attgaagagg
     8281 tgaccgatgg agccggggct ggaggtgtag atggaggtac cagcgtcatc gttgggcggg
     8341 ggggctcggt cggacgttcg gatggttctg gagtcgtggg ctttaggcct tgcaactcaa
     8401 gcttccgatc ctccaaggac gctaccagac ttcggatgag ggcagcggtc tgcttgggtt
     8461 cggcgaactt atggtggcga tgggcttgga attcctggga tttgtctcca aaggcgcgtt
     8521 taatgcactc acgaagctga agttcagtcc tggccttcgc accctccagg taaggaaacc
     8581 cttcccgtcc aagatcttcg acggtcggaa ggaacgcttg caattgctcg atgctttgcg
     8641 tcaggtcgtc gatcgcagct gttttcgacc gagcccgctc cgattttgca cgtgccatag
     8701 aatgtgcgca aagtgtagcc cacgtcctgt agacaggcaa caaaacagaa gaaagtcagc
     8761 aaagaccatt gtagccacta agctacacga aatatctcag gcggcgacgt ccagagaatc
     8821 ttcttgtccg cggaccacaa ccggttctga agatactgac tgctgagctt ccgccgaagc
     8881 cattgccgcc gcgtgaagct gccggttttt ctcgagcacg ggatcgaaca catatccgca
     8941 attgatgcag cgccaccctt gcagccacca ttcgccgcca ctttcgtgta gatcgaggaa
     9001 gtggtccgct gccatgtacc cctgacaacg tcggcaagtc ataatgcccc tcctttgtgt
     9061 acgcccgcat taagcctaac gggtacgaca gctcctgttg agacccaaag gcgttacttc
     9121 gaaaggtggg cgtagaccag gggaggcctc agtcccgggt tccgctacaa gttcagctct
     9181 tctcgaagaa agtacgtaat taccagtctt tgaatgtgcc gcatttcgtt gacagttcat
     9241 gtcacgggat accgtgcatt acgtttttgg agggctgggg caggccgaac gggaactctt
     9301 cggcgagggg cttatgaatc gacgccgacc gggagaggga agcaagaatg agtttcgagg
     9361 agaggagagg attatccgcg cttaatgtga atgagaggac cctgaattgt cgttgctttc
     9421 aacggaatct gagagaggta tgtcgagatg acctggataa tactgccttt tccaatcgat
     9481 atagctcaac tgaagtaaag caatataatt t
//
LOCUS       DASTQY010000114         6955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_52369, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000114 DASTQY010000000
VERSION     DASTQY010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6955
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52369"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VFR82_03650"
     CDS             complement(<1..859)
                     /locus_tag="VFR82_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU4500722.1"
                     /translation="MRRMTFVLTVGLLLTGCFAKIHELPAQPEHAEALFAPLPDQDFM
                     VGLAVSGGGSRAATFAAGALEALAELPVRFGTQKRSVLETVSYMSSVSGGSLATAYYV
                     TNKPPRSEPVLGEDGLSPRYREFFSIYKTAMQSNFQRSALFRQLAFFRALNPTKLAYS
                     LSEVWDGDFLGHTTFAQLYKREQRGDIPRVILNGTVYNSGRRFAFTTLPASDFDYDFI
                     ELLTKELKKPDRPVPVTPEGMAIIQKGRGGARSRGARGRNEPHTGARYPVSRPHPVAG
                     PLDGPVGRRR"
     gene            complement(859..1173)
                     /locus_tag="VFR82_03655"
     CDS             complement(859..1173)
                     /locus_tag="VFR82_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500723.1"
                     /translation="MTLMGWCPVALLLIALLVTGCAVSAVALRNTLDFNKAMPRGEGG
                     CHFRSRQAVGGGSRSAHRMGIAAGWETVAGAIAKKLVEHAGFCYFQTIRENERRKGGI
                     KE"
     gene            complement(1213..1767)
                     /locus_tag="VFR82_03660"
     CDS             complement(1213..1767)
                     /locus_tag="VFR82_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HEU4500724.1"
                     /translation="MSDQRLGSTVTDSTNALEHGFESVVFASRWIQAPLYGGLIVAEL
                     LYAYKFLVELWEMVVHIQGLEETKFMLGVLGLIDVTMVANLLTMVIIGGYATFVSKLS
                     LEGHPDRPDWLTHVDPGTIKVKLAASLIGISSIHLLKAFVDVANEDPEHIKWKIFIHM
                     TFLGSAILLAWTDKIMQKDKKHSP"
     gene            complement(1929..4280)
                     /locus_tag="VFR82_03665"
     CDS             complement(1929..4280)
                     /locus_tag="VFR82_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4500725.1"
                     /translation="MARRTQDRSMASPPAGKHEPDPSLPDTATLNTASTASGVARNGL
                     TPSHGVVTLEEETRRRLEVESTLQVSEARYRLLYDNNPSMYFTLAADGTVVSVNQFGA
                     TELGYQPDELIGESVLKVFEPAQHQAVLGQLTVCAASPMKVFQWEIQKVRKDGSTLWV
                     KERAQAVMDHQGQILTLVVCEDITARRLTEDYMRESEERWRALFEHAGVGIAQLGLDG
                     QFLRVNPRLCETLGYSSRTMTRCMFQHFVYPDDLQLILDQIRQLVSGKRPSFSMETRY
                     RRSDEVWVWVEMTVSLVRDGACVPAYFIAVMQNIHDRKQAEAGLARTTQLLQTLVRES
                     PLPIISLDLAARVTSWNQAATRLFGWSEEEVLGRELPYVPQEEAARAESLWERGLRGE
                     IHGPIELRRCRKDGKVLDLLLWPVFVHSEVGDVSTAIGLYVDQSNLKRAEEALLASEE
                     RFSKAFRSSPYPIGITDLETGVCLDINDAALEVFGFTREEVIGQTTSVLGIWPQPEAR
                     QQFVERLRKKGTIRNLEVPLKSKRGEVRQFLASTEVIVLNGKECLVTIGTDITEQKRA
                     EEALRRSEVAVLQAFEERERLSQDLHDNLLQSLYAVGMGLELTKQRIQRISRTNAKRL
                     EQSVGQLNNVIREVRSFISHVQPPVVFEQTVADTLRTLAGSFMATGAGEIVVTVDDEA
                     GLRLAPEQRTHVLAIAKEALSNSIRHTKADKRMVILRRHGNKIRLEVIDNGHGFSPLH
                     RRKQGMGMGLQNMRARARKLHGRISITSVLTQGTTVTLTIPST"
     gene            complement(4415..6223)
                     /gene="uvrC"
                     /locus_tag="VFR82_03670"
     CDS             complement(4415..6223)
                     /gene="uvrC"
                     /locus_tag="VFR82_03670"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU4500726.1"
                     /translation="MAAANPALETKLTNLPDQPGVYLFKDQSGAVLYIGKAARLADRV
                     RSYFLKGADHTPKTVLLVSQIADLETMVTRSELEALILESNLVKRHRPRFNVVLRDDK
                     QYPYLRLPIKDNFPRLSIVRRVQKDGALYYGPYTPASALRETLKVIKRVFPLATCTID
                     IDGTAERACIEFEIKRCMAPCTGQQTQTDYHRIVRQVRQFLEGHDRELVDELRTHMEA
                     AAAREEFEEAARLRDRIFKIERTLERQRITQTASIDQDVVGLARHGASVDLQLLFVRG
                     GLLIGRKDFFWLESADATDEELVRSSIEQFYNKEGQPPRELLIPLDIPDADLVGRWLS
                     EKRGETVRVLAPERGTKHQLVLLAEENAAAALAEHLRNQELDRQAGDELQRVLRLETA
                     PKRIEGFDVSNTMGNQSVASMVVWEDGRMKKSDYRRFKIATVQGANDFASMQEVVTRR
                     YGGMENLARPDLILIDGGLGQLAFARSALKELGQSDLAIIGLAKAKGEKDERVYLPGR
                     KNPIVLRANSPATHLLQRIRDEAHRFAVTYHRKLRGKTLVTSHLDQVIGVGSITRTKL
                     LKRFGSLANVAQASEEDLREAGLSERVVQQLRRELA"
     gene            complement(6266..>6955)
                     /gene="dapF"
                     /locus_tag="VFR82_03675"
     CDS             complement(6266..>6955)
                     /gene="dapF"
                     /locus_tag="VFR82_03675"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEU4500727.1"
                     /translation="RIFNPDGSEAEKSGNGLRIFARYLHATGKTKKKHFTVETKGGLV
                     TIDLHVDRHGDASAVTVAMGRATFSPGALPCTLPVKELIQQPIDAAGRSLTFTGVSVG
                     NPHCVVFKPAGESWSRQDLLTLGPKLEHHPLFPKRTNVQLAVPTGPKELTILIWERGA
                     GETQASGSSSCAAASAAVRLHLVTSPVAVKMPGGTLTIDVAPDFSLTMKGPVAEVARG
                     TVSPSFVRSLR"
BASE COUNT         1403 a         2011 c         2041 g         1500 t
ORIGIN      
        1 ctcgccgccg gccgacggga ccgtcgaggg gtcctgccac tggatgcggt cgggaaaccg
       61 gataccgagc tcctgtatgc ggctcgttcc gacctcgagc acctcgactt cgagcgccac
      121 ctcgaccctt ctgaatgatg gccatccctt caggtgtgac tggtaccggg cgatcgggct
      181 tcttcagctc cttcgtcaag agttcgataa agtcgtaatc gaaatccgaa gccggtagtg
      241 tggtgaaggc gaagcgccgt ccggagttat agaccgtgcc gttcaagatc acgcggggaa
      301 tatcgccgcg ttgctcccgc ttgtagagct gcgcgaaggt cgtgtgaccg agaaagtccc
      361 cgtcccagac ttccgacagc gaataggcga gcttcgtcgg gttcagggca cggaagaatg
      421 cgagttggcg gaacagagcg ctccgctgga aattcgactg catcgctgtc ttatagatgg
      481 agaagaattc ccgataccgt ggagagagcc cgtcttcacc gagcaccggt tccgacctgg
      541 gaggtttgtt ggtcacataa taggccgtgg ccagactgcc gccggacacg ctcgacatgt
      601 agctgacggt ttccaacacg ctgcgtttct gcgtgccgaa acgaacgggg agctccgcca
      661 gtgcttccaa ggcgccggcg gcgaatgtcg ctgcacgact gcccccgccg gaaaccgcaa
      721 gcccaaccat gaagtcctgg tcaggcaacg gggcgaacaa cgcttcggca tgttccggct
      781 gtgcgggaag ttcgtgaatc ttggcaaagc atccggtcag gagaagacca acagtcagca
      841 cgaacgtcat acgtctcatc actctttgat gcctcccttt cgtcgttcgt tctctcgtat
      901 cgtctgaaaa taacaaaagc ctgcgtgttc gacgagcttt ttcgcgatcg cgcctgcgac
      961 cgtttcccat ccggcggcga ttcccattct gtgtgctgaa cggctgccac cgccgacggc
     1021 ttgccggctt ctgaaatgac agccgccttc acctctcggc attgccttgt tgaagtcgag
     1081 agtgttgcga agagcgacgg cggaaaccgc gcagcctgtg acgagcagcg cgatgagaag
     1141 aagcgcaacc ggacaccagc ccatcagggt cacgggcacc tcaagaacga cgaccaggat
     1201 ccttgctaga gcctagggcg aatgcttttt gtctttctgc atgatcttgt cggtccaagc
     1261 cagcagaatg gccgagccca aaaaggtcat gtggatgaaa atcttccact tgatgtgttc
     1321 aggatcctcg ttcgcgacgt cgacgaatgc tttgagcaag tgaatgctcg aaatgccaat
     1381 gagggaggcc gccaatttga ctttaatcgt gccaggatcc acatgggtga gccaatccgg
     1441 ccgatctgga tggccttcaa ggctcaactt gcttacaaaa gtggcgtagc ccccgataat
     1501 caccatggtc aacagattgg cgaccatcgt gacgtcgatc agtcccagca cgccgagcat
     1561 gaatttggtt tcttcaaggc cttgaatgtg caccaccatt tcccagagct cgaccaggaa
     1621 tttataggcg tagagcagtt cggccacgat caagccgccg taaagcgggg cttggatcca
     1681 gcggctggca aagacgacgg attcaaaacc gtgctcgagc gcgttggtac tgtctgtgac
     1741 ggttgagcca aggcgttgat cggacatgat gctcccggtg tctgcgaatg cgttcaacgg
     1801 aggaaggacc tgatgggggc tgtatcctag agggctggac ggtctctgtc aaatatttcg
     1861 catcaccgac ttaacgtaaa aggaacacca tgattgaggt gcgtcgcagg agtactcccg
     1921 cagtccgctt aggtcgaagg gatggtgagc gtgactgtcg tgccctgagt caggacggat
     1981 gtaatggaga tacggccgtg gagctttctc gctcgagcgc gcatgttctg taagcccata
     2041 cccataccct gcttccgccg gtggagggga gagaacccgt ggccgttgtc gatgacttcc
     2101 aggcggatct tattaccatg tcgccgcagg atcaccatcc gcttatccgc tttggtatgc
     2161 ctgatgctgt tgctcaacgc ctctttggcg atggccagga catgagtccg ctgctccgga
     2221 gccagccgca ggccggcctc gtcgtcaacc gtgacaacga tctctccggc gcccgtcgcc
     2281 atgaacgatc ccgccagggt ccgaagcgta tcggcaacgg tttgctcgaa aactacggga
     2341 ggctgcacgt gagaaataaa tgaccgcacc tcacggatca cattattcaa ctggccgaca
     2401 gactgttcca accgtttggc gtttgttcgt gagatccgct gtatgcgctg ttttgtgagt
     2461 tcgagtccca tgccgacggc atagagagac tgcaggagat tgtcgtgaag gtcttgactg
     2521 agtcgctctc tctcctcaaa cgcctggagg accgccacct cgctgcgacg cagcgcctct
     2581 tccgcccgtt tctgctcggt aatgtcagtt ccgatggtga ccagacattc tttaccgttg
     2641 agcacgatca cttccgtgga tgcaaggaac tgacgaacct ctccacgttt cgatttaagc
     2701 ggaacctcca ggtttctgat ggtgcccttt ttccggaggc gctcaacaaa ttgctgccgt
     2761 gcctcgggct gaggccaaat tcccaggacg gaggtcgtct ggccgattac ctcttcccgt
     2821 gtaaacccga agacttccaa ggctgcgtca ttgatatcca ggcagacgcc ggtttccaag
     2881 tctgtaatgc cgatcgggta aggactcgag cgaaacgctt tgctgaaccg ctcctcgctg
     2941 gcgaggaggg cttcttcggc ccgtttgaga ttggattggt cgacgtacaa accaatggcc
     3001 gtcgaaacgt cccccacctc ggagtgaacg aagacgggcc aaagcaagag gtccagcacc
     3061 ttgccgtcct tacggcaccg ccgcagttcg attggaccgt ggatctcccc gcgcagtccg
     3121 cgctcccaaa gcgactcagc cctggccgct tcttcttgag gaacgtaagg cagttcacgg
     3181 cccaagacct cctcctccga ccatccgaac aagcgtgttg ccgcttgatt ccaacttgtg
     3241 acacgggcgg cgagatccaa gctgatgatc ggaagggggg actcacggac gagtgtttga
     3301 agcagctgag tcgttctagc caggccggcc tccgcttgtt tccgatcgtg gatgttctgc
     3361 atgactgcga tgaaatacgc gggaacacaa gcgccgtcgc ggaccagaga gacggtcatc
     3421 tcgacccaca cccagacctc gtcgctgcgg cgataccgcg tttccatgga gaacgaggga
     3481 cgtttgccgc tgaccaattg gcggatctga tcgagaatca gctggagatc gtcgggataa
     3541 acgaagtgct ggaacatgca ccgcgtcatc gttcttgatg aatatcccag cgtctcgcag
     3601 aggcgaggat tgactctgag gaactgtcca tccaaaccga gttgggcgat tccaacgccg
     3661 gcatgttcga agagcgcccg ccagcgttct tcgctttccc gcatatagtc ttctgtgagt
     3721 cggcgtgcgg tgatgtcctc acacacgacc agggtaagga tctgtccttg atggtccatg
     3781 acggcctgcg cccgctcttt cacccacagc gtgctgccat ctttccgcac cttttggatt
     3841 tcccactgaa agactttcat tgggctggcc gcgcagacgg tgagttgtcc gaggacggct
     3901 tgatgttgag ctggctcaaa gactttcagc acggactcac cgatcagctc atcgggttga
     3961 tatccaagct ccgtggcgcc gaattggttg acggagacga ccgttccatc ggctgcgagc
     4021 gtgaaataca tcgacggatt attgtcgtac agcagccgat agcgagcttc gctgacttgc
     4081 agcgtggatt cgacttcaag acgacggcgg gtttcctcct ccagagtgac gaccccatga
     4141 ctgggggtaa ggccgtttcg agctacgccg ctggcggtag aggcagtatt tagcgtcgct
     4201 gtgtccggga ggctcgggtc aggctcatgc ttgccggctg ggggtgaggc catcgagcgg
     4261 tcctgtgtgc ggcgggccat cggtctcctt gaagaaggcg gcacacgaat cgagtggttt
     4321 ggcagctcag tgttcagggg ctagcatagg gcggttggcc tcgaaattcc agtaggtgct
     4381 gatcgtcctc ctccactgca cagcgggcag gtcctcacgc taattcccgg cggagttgct
     4441 ggacgacgcg ttcgctcaac cccgcttcgc gaaggtcctc ttcactggct tgagccacat
     4501 tcgctaggct tccgaatcgc ttaagcagct tggtgcgggt gatcgagcca acgccgatta
     4561 cctgatcgag atgggaggtc acgagcgttt tcccgcgaag tttgcgatgg tatgtgacgg
     4621 cgaagcgatg cgcctcgtcg cggatccgtt gtaggagatg agtggccggc gaatttgcac
     4681 gcagcacgat tggatttttt cgcccgggga gatacacccg ttcatctttc tcccccttcg
     4741 ctttggccaa tccgatgatg gcaaggtcgg actgtcccaa ttctttgaga gcggatcttg
     4801 cgaaagccaa ctggcccaac cctccgtcga tcaagatcag atccgggcga gcgagattct
     4861 ccatcccgcc gtatcgtcga gtgacgactt cctgcatgct cgcaaagtcg ttggcgcctt
     4921 gaacggtggc gatcttgaac cggcggtagt cggacttttt catgcgaccg tcttcccaga
     4981 cgaccataga cgccactgac tggttgccca tcgtattgga aacatcaaag ccttcaatgc
     5041 gcttcggcgc cgtctcaagc cggaggacac gttgcaattc atctccggcc tgccggtcta
     5101 gctcctggtt gcgcaggtgc tcggccagcg cggctgccgc gttctcttcc gccaacagca
     5161 cgagttgatg cttcgtgccg cgctccggtg ccagcacccg gacggtttca ccgcgtttct
     5221 cggacaacca gcgacccacg aggtcggcat ccggaatgtc cagcgggatc agcaattccc
     5281 ggggtggttg cccttccttg ttataaaact gttcaatgga cgagcgaacg agctcttcat
     5341 cagtggcatc ggccgattcg agccagaaaa agtccttgcg gccaatcaac aaacctcccc
     5401 gcacaaagag cagttgcagg tcgacggaag ccccatgtcg tgcgagaccg acgacgtcct
     5461 gatcgatgga agcggtttgc gtgattcgct gccgctcgag cgtgcgctcg atcttgaaga
     5521 tccgatccct caacctggcc gcctcctcaa actcctcacg agccgcggcg gcttccatgt
     5581 gagtacggag ttcatcaacc agttctcggt cgtgtccttc caaaaactga cgtacctgtc
     5641 ggacgatcct gtgatagtcc gtctgcgttt gctgtcctgt acagggcgcc atgcagcgtt
     5701 tgatctcaaa ttcgatacag gcccgttcgg cggttccatc aatgtcaatc gtgcaggtgg
     5761 cgagcggaaa tacacgtttg atgactttga gtgtctcgcg cagagcactt gccggcgtat
     5821 aaggtccgta atagagggcc ccatccttct gcacccgtcg gacgatggac agcctgggaa
     5881 agttgtcctt aatggggagg cgaagatagg gatactgttt gtcgtcacgc agcacgacat
     5941 tgaagcgggg ccggtgacgt ttaacgagat tgctctcaag gatcaaggcc tccagttcgg
     6001 aacgcgtgac catcgtctcc agatcggcga tctgactgac cagtaaaact gtcttgggcg
     6061 tatggtctgc gcctttgaga aaatacgacc gaacgcggtc ggcgagccgg gcggccttgc
     6121 cgatgtacag caccgctccg gactggtcct taaacagata gacgccgggc tggtcgggca
     6181 ggttggtcag tttggtttca agtgcgggat ttgctgcagc catgcctcat tctacgagtc
     6241 cgactgcgta ccgggcaagg cagcgctatc tgagcgagcg cacgaaggag gggctcacgg
     6301 tcccgcgcgc aacttccgcc accgggcctt tcatcgtgag actgaaatcc ggcgcgacat
     6361 cgatcgtcaa ggtccccccc ggcatcttta ccgccaccgg actggtgacc aagtggagcc
     6421 ggactgcggc gctcgcggcc gcacaggaag aggaaccgga ggcctgcgtt tcgccagccc
     6481 cgcgttccca aattaagatg gtgagctcct tggggccggt tggaaccgcc aattggacat
     6541 tggttcgctt gggaaagaga ggatgatgct cgagcttcgg acccagggtg aggaggtcct
     6601 gtctggacca ggactctcca gccggtttga acacgacgca gtgcggattg cctacgctga
     6661 cgccggtaaa tgttaatgag cgcccggctg catcgatcgg ctgctggatc aactccttga
     6721 ccggcagcgt gcaaggcaac gcacccggag aaaatgtggc acgccccatg gcgacggtga
     6781 ccgcactggc gtcaccgtgc cggtcaacat gtaaatcgat cgtcacgagc ccgcctttcg
     6841 tctccaccgt aaaatgtttc ttcttcgtct tgccggtcgc atggagataa cgggcgaaga
     6901 tgcggaggcc gttgccggat ttttccgctt cgctgccgtc cgggttgaaa atgcg
//
LOCUS       DASTQY010000115         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_162166, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000115 DASTQY010000000
VERSION     DASTQY010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162166"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFR82_03680"
     CDS             complement(<1..299)
                     /locus_tag="VFR82_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500728.1"
                     /translation="MKPVSGLRCLWAFGSLVPNRLDRRPRFTLTNQRQQVRFKACPVF
                     GRMTEQELNESALSRAKMPMHSAAGKTVQNTERLLGKETFEFVGSHGFSLKFNPS"
     gene            369..1094
                     /locus_tag="VFR82_03685"
     CDS             369..1094
                     /locus_tag="VFR82_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4500729.1"
                     /translation="MPLPQPFVPDPWQEDSLAKLQSGDVIVSVPTGSGKTYVAVEAAR
                     RAMDENRTVIYTSPLKALSNTKYTEFCRIFGADRVGILTGDRRDNGQAPLLIMTTEIL
                     RNLLYDAAGGEIDLRLDTLGLVILDESQYLADPERGVVWEETIIFCPTQARLLLLSAS
                     IGNPHDIADWLTSIRSTPCHLIKHSKRTVPLRAGYLHPDGKLTPLFRTIGIPSGHPVH
                     LHPEAKRLFARYEDETMPSGRRR"
     gene            complement(1201..2220)
                     /locus_tag="VFR82_03690"
     CDS             complement(1201..2220)
                     /locus_tag="VFR82_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500730.1"
                     /translation="MMLRSRLAAGLLALLSLIGGATHLYAQDGGSERRPRVEVGLGGW
                     VITQGETKWAHNASSIAGLGNPTSKLTYKDTGTNIVEGTVRFWVTPSWFGRLTAGYGT
                     VGGGRLTDDDYLSVDGGAPSSRTFSDLKGDSTWYLNADVGRRIVEFPQSRGWLDLFAG
                     YQYWYQRYSANGLGQASCSTAGQTLDLDPGSPGNQTLCSPNTTVSSSIQAITNTTQWH
                     SIRLGASTEYRFTRRLSVLGTVVLIPVSIVDNTDVHHLRPDLNQNPSISMVGYGIGAD
                     VDVGARYMIFRNFYANVGYRLWWNRTVDGTVTFHNAIGGSDEFPLTELQSFRHGVTFG
                     ISYAF"
     gene            complement(2217..2483)
                     /locus_tag="VFR82_03695"
     CDS             complement(2217..2483)
                     /locus_tag="VFR82_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500731.1"
                     /translation="MPSLHVVADDEALLAKTSTRAVPVKLIVQWCVGIFAAFVLGTWL
                     VWWSAVVQRTFTTEDIRSACQRVMPESVERCIDTVTIQRGGARR"
     gene            2730..2805
                     /locus_tag="VFR82_03700"
     tRNA            2730..2805
                     /locus_tag="VFR82_03700"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2763..2765,aa:Arg,seq:acg)
     gene            complement(2919..>3125)
                     /locus_tag="VFR82_03705"
     CDS             complement(2919..>3125)
                     /locus_tag="VFR82_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500732.1"
                     /translation="VEPCVDYVPLSEQQRRFHSSWNFAVLRDSRPFWRELVEQAVDHK
                     FVATVGTSRIVKTNVNAALVGLLI"
BASE COUNT          691 a          942 c          849 g          643 t
ORIGIN      
        1 ttcgatgggt tgaactttag actaaagcca tgactaccga cgaactcgaa cgtctccttg
       61 cccaacagcc gctcggtgtt ctgcaccgtc ttgcccgcgg cagagtgcat cggcattttc
      121 gcgcgggaaa gcgccgactc attgagctct tgctccgtca ttcggccgaa aaccgggcag
      181 gctttgaatc ggacttgctg gcgctgatta gtgagcgtga agcgaggcct gcgatccaac
      241 ctgttcggca ccagcgatcc gaacgcccaa agacatcgca gcccggacac cggcttcatt
      301 cagcgcacca tgagccggat catcacgagc ctgcggcgtc gttagccgcc tggctcgaag
      361 gaatcggcgt gcctctccct caaccgttcg ttccggatcc gtggcaggag gactcgctgg
      421 ccaagctgca aagcggagac gtgatcgtca gcgtgccgac aggcagcggg aaaacgtatg
      481 ttgcggtgga agcggcacga cgtgccatgg acgaaaaccg caccgtcatt tatacctcgc
      541 cgctgaaggc gctttcgaat acgaagtaca ctgaattttg ccgcatcttt ggagcagacc
      601 gcgtcggcat cctgacgggc gatcgtcgcg ataacgggca ggctcccctc ttgatcatga
      661 ccactgagat tctgcgcaat ttactttacg acgcagctgg tggcgagata gacctgcggc
      721 tcgatacgct cggattggtc atcttggatg aatcgcagta tctggccgac ccggaacgtg
      781 gagtcgtctg ggaagagacc atcatctttt gtccgacgca ggcgagactg ttgctcctct
      841 ccgcctcaat cgggaatccg cacgacattg cggactggct cacgtccatc cgttccacgc
      901 cctgtcactt gatcaagcac agtaagcgaa cggtgccctt gcgtgccggg tatctgcacc
      961 cagatggcaa gctcacgcca ctcttccgca ccatcgggat acccagcggc catccggtgc
     1021 atttgcatcc tgaagccaaa cgactcttcg cccgctacga agacgaaacg atgccatctg
     1081 gcaggcgccg ctgagcagct acgcccaatc tccaaccgac gggatctcgc aggacgtgag
     1141 gctcttaacg gagcgcgacg ggaaggtcac gcctctggtt aggacaagct ggtccttggt
     1201 ttagaatgcg tagctaatgc cgaacgtgac gccgtgccgg aagctttgaa gctccgtgag
     1261 gggaaactcg tctgagccgc cgatggcatt gtgaaaggtg acggttccgt cgacagtgcg
     1321 gttccaccag aggcgataac cgacattcgc atagaagttt ctgaaaatca tgtaacgggc
     1381 gcccacatcc acatcagccc cgatgccata acccaccatg gagatgcttg gattttggtt
     1441 caggtctgga cgcaaatggt gaacatctgt gttatcgaca atgctcacag gaatcaaaac
     1501 caccgtcccc agaacactca atcgtcgcgt gaagcgatac tcagtcgatg ctcctaatct
     1561 gatggagtgc cactgagtcg tgttggtgat agcttgaatg gagctgctga cggtggtatt
     1621 ggggctgcag agcgtttggt ttccggggct gcccggatca agatccagcg tttgaccagc
     1681 agtcgaacaa ctcgcctggc caaggccgtt ggcgctgtaa cgctgatacc agtattgata
     1741 tccagcgaaa aggtccagcc agccgcgcga ttgagggaat tctacgatgc gccggccgac
     1801 gtcggcgttc agataccacg tgctgtcgcc cttgaggtca ctgaaggtcc gggacgacgg
     1861 cgctccacca tcaacggaca aataatcatc gtccgtgaga cgtccgcctc cgaccgtgcc
     1921 atagccggca gtcaaacgac cgaaccatga tggcgtgacc caaaaacgga ccgtgccctc
     1981 gacgatgttc gttccggtgt ctttataagt gagcttcgaa gttggatttc ccaaaccggc
     2041 aatgctcgag gcattgtgag cccacttggt ctctccctgg gtgatgaccc agccacccag
     2101 gccgacctcc accctcggac gccgctcact cccgccatcc tgcgcgtaga gatgagtcgc
     2161 cccccctatc agcgaaagca aagcgaggag gccagcggcc aggcgcgacc gcagcatcat
     2221 cgtcttgcgc ctccccgctg gatggtcacc gtatcaatgc aacgttcgac cgactccggc
     2281 atcactcgct gacaggccga ccggatatct tcggtcgtaa acgttcgctg cacgacagca
     2341 gaccaccaga ccagccaggt tcccaagacg aaggcggcaa agatcccgac gcaccattgc
     2401 acgatcagct taaccgggac cgctcgtgtg gaagtcttgg cgagcagtgc ctcgtcatcc
     2461 gccactacat ggagactggg catagtaaaa ctccagcagg tctaggacac cggcccctat
     2521 cctactggag tttgatcgat ctgaacatta ttttgtggtt cttgtgtcgt cgcgatggca
     2581 tgcgagcgct tgacccctgg cccccggacc tgtataaccg ctggctgcct cttcagcatg
     2641 gctgagaccg cgtgttgtgc ctgagatttc ctattcacgt cactcagatc tcggcattca
     2701 ctcactcagc actccagcag ttcttcagag cgcccgtagc tcaatggata gagcaccaga
     2761 ctacggatct gggggttaca ggttcaactc ctgtcgggcg caccatttct caataacttc
     2821 tgaagcacaa tcttacgcaa ataatttcct aagcgaaggg ctagtctcct tgcgaaaact
     2881 ggcccgcttg gtgggaagcc ggatcaacgt cacgacactt agataagcaa cccaaccagc
     2941 gctgcgttga cgtttgtctt gacgatccgt gaggtaccca cggtcgccac aaacttgtgg
     3001 tcaacagcct gttcaactag ctctcgccag aacggacgag aatcccttaa cactgcgaag
     3061 ttccaagacg aatggaatcg cctttgctgc tcgcttaaag gcacgtagtc aacgcaaggc
     3121 tcaac
//
LOCUS       DASTQY010000116         4774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1269301, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000116 DASTQY010000000
VERSION     DASTQY010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269301"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="VFR82_03710"
     CDS             <1..1356
                     /locus_tag="VFR82_03710"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEU4500733.1"
                     /translation="SRERVEFFASIKLAMFLFLFIAITATIGTVIQQGERAETYIQEY
                     GEDAYRWFLRLGFTDVYHTWWFTGLLGLLCVNSLTCFYKRFPAVWRSMKQDKVSVSLA
                     FIKGMKQQAEVVVQGTKESIAQQLAQHYVGKGYRVLAKNEAGEVTLYATKGVMGRVGA
                     HMAHLSATVIVLGGLLGSYYGFQEFGVCLEGQTYHIPRGNFDLKVDKFWIDYHENGAV
                     KSYNSTLTVLESGESKITKTITVNDPLVYKGIWFYQSSYGDAWDQIEVARVNIKDKQT
                     EKIVKTIDLEWQKEHTLSDLNLKLAITDFVADFAFNSTEKKVYSKTIEHANPAIKLTV
                     NERSTVQATPWIFYQFPDLFEIKDSKYQFELIGYKPKKYTGLQIAKNPGINIVWIGST
                     MIVVGITLSSFIYHRRLWTKIIPSGDSVTVHVGGTTHKSQIDFQKEFRKLTEKIRTLE
                     A"
     gene            1497..2411
                     /gene="ccsB"
                     /locus_tag="VFR82_03715"
     CDS             1497..2411
                     /gene="ccsB"
                     /locus_tag="VFR82_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEU4500734.1"
                     /translation="MARSLFLFDMTFWLYLASLTLYVAYLFARRPAMAFAPAGHPTED
                     FDERDGAWATQLGQVATLVTVFGWMVNTAALLTRAFERLEHSGTFAPWSNQFEAMAYV
                     SWAIILGYVLLELRYKIKAIGAFVVGIGFIAMGAASLLPYRYQTAEPLVPALNSYWIY
                     IHVSVTLTSYAAFAMAGGLGVMYLFKERAEQRGSRSRFYAAFPDLETIDELGYKAIMI
                     GFPMLAFGIILGAMWANYAWGGYWSWDPKETWSLIVWLIYGAYIHARMTRGWEGRRAA
                     IYAVFGFLMVIFCFWGVNFLLSGLHAYA"
     gene            2683..3258
                     /locus_tag="VFR82_03720"
     CDS             2683..3258
                     /locus_tag="VFR82_03720"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU4500735.1"
                     /translation="MLILAVGTAILVAVFTLVWLQSAKYEPLVVGKPAPDFSLTDLNE
                     KPYRLSDFRGKVVFLNFWATWCKPCREEMPSMEILNKNFEKDGLVILAVSIDRVTTTK
                     DIPPFVKGLNLTFPVLVDSWGRTDKPYKRMGVPETFIIDQEGIIQEIVIGPRDWTRLD
                     SLQILTKLLHVTPKTAGTPAPLARASMVDQG"
     gene            3255..3791
                     /locus_tag="VFR82_03725"
     CDS             3255..3791
                     /locus_tag="VFR82_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU4500736.1"
                     /translation="MKFLVTAALLSVFVTMAVSASSQAGSAASVAQRGIVKPGEPAPN
                     FQLRDMNGRIVSLSELRGKVVLLNFWATWCGPCRVEMPAMERLYRTYDRKDFEILAVS
                     TDAQGVAVTRPFQEENKLTFPILHDADFRVGLSYGARTLPMTFMVDRQGVVRQHIFGA
                     RDWEAPEAHQLIEMLMKS"
     gene            3801..4547
                     /locus_tag="VFR82_03730"
     CDS             3801..4547
                     /locus_tag="VFR82_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEU4500737.1"
                     /translation="MSESLTQISMIAAFSAGLLSFVSPCVLPLVPSYISYITGLSIEQ
                     LTDANARSQFKRAIVVNALLFIAGFSVVFIAFGASASFVGQLLMTYQEHIRRIGGLLI
                     VIFGLYLLGILNINFLQMEHRFQFRNRPVGYLGSFLIGIAFAAGWTPCVGPVLGTILL
                     YASTTDSLLDGVFLLTSYSLGLGLPLFLTALGVDRFLAYFKQARAYLWGVSTVSGVLL
                     VIVGVMIYANSLTMITSFLERHGIGWYLGQ"
BASE COUNT          974 a         1261 c         1367 g         1172 t
ORIGIN      
        1 tcgcgcgaac gggtcgaatt cttcgcctcc atcaagttgg cgatgtttct gtttctgttc
       61 atcgcgatca cggcgaccat cgggaccgtc attcagcagg gcgagcgggc tgagacctac
      121 atccaggaat atggagaaga cgcctatcgc tggtttcttc gtcttggctt cacggacgtg
      181 taccacacgt ggtggtttac cggactgctc gggctgctgt gtgtcaactc gctcacctgt
      241 ttctacaagc gtttcccggc cgtctggcgg tccatgaaac aagacaaggt cagcgtctca
      301 ctcgctttca ttaaagggat gaaacagcag gccgaagtcg tggtgcaggg caccaaagaa
      361 tcaatcgcac agcagttggc ccagcactac gtcgggaagg gctatcgggt cctggccaag
      421 aatgaggcgg gggaagtgac gctgtacgcg acgaaaggag tcatggggcg cgttggcgcg
      481 cacatggcgc atttgagcgc cacggtgatc gtgctcggcg gactactcgg aagctattat
      541 gggttccagg aatttggcgt ctgtctggaa ggccagacct atcatattcc tcgtggcaat
      601 ttcgatttga aagtggacaa gttctggatt gattaccacg agaacggcgc ggtcaagtcc
      661 tataacagta cgctcaccgt gctcgagagc ggcgagtcga aaatcacgaa gacgattacc
      721 gtcaacgatc cgcttgtgta caaaggtatc tggttttatc aatccagcta cggggatgcc
      781 tgggatcaaa tcgaagtggc gcgggtgaac atcaaagaca agcagaccga gaaaatcgtc
      841 aagaccatcg acttggaatg gcaaaaggag cacacgctct cggatttgaa cctgaaactg
      901 gccattacgg actttgttgc agactttgcc ttcaattcaa cggaaaaaaa ggtgtattcc
      961 aagacgattg agcatgccaa cccggcgatc aagctgacgg tcaacgagcg cagtacagtt
     1021 caggcaactc cttggatctt ctatcaattc cccgatttgt tcgagatcaa agactcgaaa
     1081 taccaatttg aattgatcgg ctacaagccg aaaaagtaca cagggctgca aatcgccaag
     1141 aatcccggca tcaatattgt gtggatcggt tccacaatga tcgtcgtggg gatcacgctc
     1201 tcgtcgttca tctaccatcg ccggttgtgg acgaagatca tcccatcagg ggacagcgtg
     1261 accgtgcacg tcggcggcac gacgcacaag agtcagatcg attttcagaa ggagtttcgg
     1321 aagctgacgg aaaaaatacg aacacttgag gcgtagccga ccggcttctt tctggctggg
     1381 tcttctttga aggggtgccg caccggaaga ggaagcggcc tgccagtggt caaaggccac
     1441 cactcttggg tatgataaac gggttcgatt gaacccagtg aacgacggag gatacgatgg
     1501 cccgttcatt gtttctgttc gacatgacct tctggctcta tctggcttcc ctgaccctgt
     1561 atgtcgccta cttatttgcg cgccggcctg cgatggcttt cgcacctgcc ggccatccga
     1621 ccgaggactt cgatgaacgg gacggagctt gggccactca actggggcaa gtggccacgt
     1681 tggtgacggt gttcggctgg atggtcaaca ctgcggcgtt gctgacccgc gcgttcgagc
     1741 ggttagagca ttccggtacg ttcgctccct ggtccaacca gttcgaagcg atggcctacg
     1801 tgtcctgggc gatcattctc gggtacgtgc tgctggaact ccggtacaag atcaaggcga
     1861 tcggcgcatt tgtcgtcggc atcggcttca ttgcgatggg agctgcctcc ctccttccat
     1921 atcgctacca gacggctgag cctctcgtgc cggcgctcaa tagttattgg atttacattc
     1981 acgtctccgt gaccttgacc agctatgcgg cgttcgcgat ggccggtggt cttggagtca
     2041 tgtatttgtt caaggaacgg gccgagcagc ggggcagccg gtcacgtttt tacgctgcct
     2101 tcccggacct ggaaacgatc gatgagctgg gctataaggc cattatgatc ggatttccca
     2161 tgctcgcctt cggcattatt ttgggcgcta tgtgggcgaa ttacgcctgg ggcggctatt
     2221 ggagttggga cccgaaagaa acctggtcgc tgatcgtctg gctcatctat ggcgcctata
     2281 tccacgcacg aatgacgaga ggctgggagg gccggcgtgc cgcaatctac gccgtattcg
     2341 gcttcttaat ggtcattttt tgcttttggg gtgtgaattt cttactgtcc ggtctgcatg
     2401 cctatgccta atgagttccc ctcacgctcc aatgcaagct tattggagca acataacttt
     2461 ccgctcgcat gtgattgaag cgggcggctc aagcgaactt ggtatgtacc tcctcggttt
     2521 ctcgctcact gcggccttgc ttgatgaatg gtgcgtcttc gcgcgccggg gtgggcgggt
     2581 gataaaagaa ccgttttgag catcccgcaa gacaggcgtg gagaatcaac cgtggtagag
     2641 caatcaattc cagtcacaac tccagggaac ggaacctcac gcgtgctgat tttggccgtc
     2701 ggcacggcaa ttctcgtcgc cgttttcact ctggtttggc tgcaaagcgc gaagtatgag
     2761 cccctcgttg tcgggaaacc ggcccctgat ttttcgctta ccgatctgaa cgaaaaaccc
     2821 tatcgtctgt ccgattttcg ggggaaggtt gtctttttga acttctgggc gacctggtgc
     2881 aagccttgcc gcgaagaaat gccttcgatg gaaatcctga acaagaattt tgaaaaagat
     2941 gggctggtga ttttagccgt cagtatcgac cgcgtcacca cgacaaagga cattcctccg
     3001 ttcgtcaagg gcttgaacct gacctttccg gtcttggtcg attcctgggg gcggaccgac
     3061 aaaccctata agcggatggg tgtccccgaa acgtttatca tcgatcagga aggcatcatt
     3121 caagaaatcg tcattggacc gcgtgattgg acgaggctcg acagcctcca gatattgacg
     3181 aagctgcttc acgtcactcc gaagacggcc gggactcctg caccacttgc aagagcttcg
     3241 atggtggatc agggatgaaa tttcttgtga ctgccgcact cctgtctgta ttcgtgacga
     3301 tggccgtatc ggcctcgagc caggccggca gcgcggcatc agtggcgcaa cgtggaatcg
     3361 tcaagcccgg cgagccggcc cccaactttc agcttcgcga tatgaacggg cggattgtct
     3421 ctctgtctga attgcgaggg aaggtggtgc tgctcaattt ctgggccacg tggtgtgggc
     3481 cgtgccgggt agagatgccg gccatggaac gtctttaccg gacgtacgat cgaaaagact
     3541 tcgagatcct cgccgtctcg accgatgcgc agggagtggc ggtcacgagg ccgtttcagg
     3601 aggagaacaa gctcaccttc ccgattcttc acgacgccga ttttcgcgtc gggttgtcgt
     3661 atggggcacg gacgctgccg atgacattca tggtcgaccg tcagggcgtg gtgcggcagc
     3721 atattttcgg cgcccgtgac tgggaagctc ccgaagcaca ccagttaatt gagatgctga
     3781 tgaagtcata gagatttacc ttgtccgaat cgctgactca gatttcgatg attgcggcct
     3841 tttcggcggg attgctctcg tttgtctcgc cgtgcgtgct gccgcttgtt ccctcctata
     3901 tttcctacat cacaggttta tcaatcgagc agctgaccga tgccaatgcg cggtcgcagt
     3961 tcaagagagc gatcgtcgtg aatgctcttt tgttcattgc cggcttttcc gtcgttttca
     4021 ttgccttcgg agcttccgcc agtttcgtcg gtcagctgct catgacctat caggagcaca
     4081 tccggcgcat tggtggcctg ctgatcgtca tttttggcct ctatctgctc ggcattctca
     4141 acatcaattt cttgcagatg gaacaccggt ttcaattccg gaaccggccg gtgggatacc
     4201 tgggttcgtt tttaatcgga atcgcgtttg cagccggttg gacgccctgt gtcgggccgg
     4261 tactcggcac catcttgctt tatgccagca ccaccgattc tttgctcgac ggcgtgttct
     4321 tgctgaccag ttattcgctg ggtttgggtt taccgctgtt cttgacggcc ttgggtgtcg
     4381 atcgttttct ggcctatttt aagcaagcgc gcgcctacct gtggggagtg tccactgtca
     4441 gcggtgtgtt gcttgtcatc gtcggcgtga tgatctatgc caattcactg acgatgatta
     4501 cgagtttttt ggagcggcat ggcatcggct ggtatctagg tcagtagctt tcatagaccc
     4561 gtccccattt ctctccatat tcttcgacag actggtcaaa cagggcgtca ttcttacccg
     4621 cccacccagg tgcaccgaga cgcgcccttt accctggaag gccgcagcag gcgaaacgcc
     4681 gaggtatacg ctggttggta tgtcgaggga ttgagcgaag cgagagcgca gccgacggcc
     4741 tgtttcactg gtctggggtt agcaccaggc gccg
//
LOCUS       DASTQY010000117        10028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1159997, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000117 DASTQY010000000
VERSION     DASTQY010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10028
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159997"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2883
                     /locus_tag="VFR82_03735"
     CDS             <1..2883
                     /locus_tag="VFR82_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU4500738.1"
                     /translation="PLTQSRQVQVDITETEHTVVAPAWSTRDSPLDAAELFQLARELG
                     ATLDISAPAQGPFRITVRFPTISFPSPPRPHAMPESGVPVAIQPPTVTATPPTRDISQ
                     ATPARTDRRSYARVPTALQAKVHSGSSEWDGTVVNLSVGGACIQMPGDFPSVALQEAH
                     VTVTTSVATLELSGFIYQRRLPDAIAQPSLVPASHLIVVLNRTPQIEAAVLASLITAT
                     HERSLLFALDIRLAAAPIGNLEAGRDLAFNLTDDDRREAVRIPLKLRARLEASRGPAP
                     TGHIAAQLTNLSRSGAALLIQARPEDLQDSLRIHFAPPHRGEAGPLETGAPDTILPAH
                     VVWSAPDRTASTALHVPGTDFAARAGLRFDPLTPYAERELNRVIRQYLRAQRGSETSS
                     AASIVSIPRECRNARGQAISILDDHHLQLTDAGRPVIIIAPGYGHIALDYAALAYYLA
                     EHQFRVLRYDPTNHLGNSDGELQHTTLRNLQHDLDKVIEFVRDTWPQAPVLVIASDLG
                     ARAALKSAAQIRPLDLLLLVNPAVDVRHLLMTAHGHDLLADYQFGLRRGVCNVLGLNV
                     NVDQFVGDLIAGRCADLDSTLEDLRLIRSPLCIATSPAVPAALPPADLPHAFMTALGA
                     HTKLVNLSTPLTERRLDVHGAPPAAFKQVLMQIGSVIPVQGLSAQQEISAHPFVARQR
                     RIEQEYTFLRHEGSQINREALCAAYLTQLPQLGNLHEYRKLLDDLYGLMSPLDSGSVL
                     VDAGVGQSDLTRAALVNHTYRAGQASPLGRRPPLMIGVGRARERLVQSRHAVLLLQRE
                     LASGFVGRPSAMPPLAIGWNQADWMSSLPFKTGSVTRLVCNLSLPYAPSPAIALREWH
                     RTLHPEGSLIVTTFHPDTDLSPLYRRHLRQANQDEFSAQAQPLLHYFARLREAIRHRL
                     LHTFDEAGLASLLRHCGIESFRILPIFDGQALVAVVGKQNTSSSIR"
     gene            3014..4258
                     /locus_tag="VFR82_03740"
     CDS             3014..4258
                     /locus_tag="VFR82_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4500739.1"
                     /translation="MGSDATPSSIDLLDSVTRFSHEIGTLSDLGALGERIIRELCRVS
                     STRHGVLYVLDREHEHFRRACIVGVTAESCCPAILALTDTIPKRLSSAPRTVTGSCCV
                     QIRREDAWDDAAAMQIEWALPHVSRGRLIAFSLFGRSLSQDSDSPSTLSLLNALAQAA
                     TNALDTRMLLEDLQRSHLLMKRTDRLKSLETIAGGFAHEIRNPLTSIKTFIQLAPERK
                     DDPDFIREFSRVVLDDVYRIERLIEEILDYARYMEPKLTEEDPNDIVSSCLYFIEVKA
                     DSRGIKIEKELVSDLPRVMLDRQQIKQVFLNLFLNALDAMRDCGGVLRVRTRNLIKPG
                     GTSWVQIETQDTGHGIQEANLEHIFDPFFTTKHESGEHEGTGLGLTIVHQIIEEHHGE
                     IQVTSKVGSGTTFLVNLPAIPS"
     gene            4255..5700
                     /locus_tag="VFR82_03745"
     CDS             4255..5700
                     /locus_tag="VFR82_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU4500740.1"
                     /translation="MTTKTAIESSLKKRVLLVDDEARVRASLKAVLEPSYEVIQAADA
                     QEGLDLFHKEAPHVVLLDVILPGTDGLSVLQTLRTEQNTTPVIMLTGTKSVKTAVDAM
                     KLGAADYLSKPFDVEELRIVVDRALNSHELEREVKQLRAQVTQRYAFHNLIGKSRPMQ
                     EIYGKIEQVADSRTTVLITGESGTGKELVAKALHYNSGRRDRPFVALNCAALPETLIE
                     SELFGHEKGSFTDATARRVGQFELANSGTLFLDEIGDLSAMTQAKLLRVLQEREFTRI
                     GGVQAIKVDVRIVAATNKNLEELVRKGQFREDLYYRINVIALYLPPLRERGEDVPLLA
                     KHFLSKRIEEDHRPSQEFSKEAIDLLARYPWPGNVREMENIIEQAFIWSKGSDVVTPE
                     HLPTILRNDTRSSSLRDDTLAGRLSLEKAVMEFEREIILDALKKNNYVQTHAASQLGI
                     SRRMLKYRMDTLGIGRPDSESPPEPEPIAQE"
     gene            5904..7055
                     /locus_tag="VFR82_03750"
     CDS             5904..7055
                     /locus_tag="VFR82_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU4500741.1"
                     /translation="MTIPALRNMPAQKPTVLVIDDEAGPRDALKVILRPFFNIQSAES
                     AQAAINVLNHQQVDLITLDQKLPDRQGMDLLQEIKQDHANIEVIIITGYGSLKSAMEG
                     IRHGAAGYLLKPFNVTELITLVNQTLEKKQRLDYLRRFLGTSTGLWGSDRECAQAWHE
                     MREGYEAIGKCSGQSLSAPDDASNLLPMLSDLLEAKDRQLLNHCSRVSFYATLLANRT
                     NLTVEEQKSLALGAFLHDIGKVGKEVYRFADDEIVATNTTHELRSHPEIGAKMVAPLG
                     LPAATGQIIAYHHERWDGSGYPHGLKGEGIPILARIVCLAQTFDHLTAELPDRIALPI
                     DEASSFIAARAGSYFDPQLTALFTQVVQECKASLPAMAIATSPSTRSDV"
     gene            complement(7130..8815)
                     /gene="recN"
                     /locus_tag="VFR82_03755"
     CDS             complement(7130..8815)
                     /gene="recN"
                     /locus_tag="VFR82_03755"
                     /inference="COORDINATES: protein motif:HMM:NF008121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU4500742.1"
                     /translation="MLAELRIINFALIEQLHIQFQSGFTVLTGETGAGKSLLIDAIGL
                     LVGGRGSAEQIRSGEDEAHLEASFHLPLSHPLLEQLRTQDLIGPRETDLILKRILSRS
                     GRHRVYVNGSLCPLRVLEDLGGMLVDIHGQHEQQSLLASTKQLHAVDAFGSTESLRLQ
                     YETAYREWRDLTAHIESLRCAGVERARMEELLRFQVQEIEQAGLSVDEEDRLKMERQR
                     LLHAHRLRELAESSYSALQGDERGTLSQLATVGRFVSELAQTDPVMGECEAQVRQAII
                     HLKDVTACLRGYADDLEADPARQATVEDRLDLIHRLKTKYGGSTEAVLETGKRAKQEL
                     ETLEQHEAQMSGVMKRLKEVEPRVIDKAKQLTKKRREAAKRLTTLVAKELAAVRMAHT
                     LFDIVVTADEELDRFGPTGRDRVEFLLSSNVGEPPRPLNRVASGGELSRIMLALKTVL
                     AETDQVPVLVFDEIDTGVGGAVAAAMGTRLRKLGAYHQVFCITHLPQVASQAEHHVIV
                     EKGQDGKRTTTSVRLLSGVGREEEIARMLGGETVTKKIRETAAELIAGARSKR"
     gene            complement(9045..9392)
                     /gene="trxA"
                     /locus_tag="VFR82_03760"
     CDS             complement(9045..9392)
                     /gene="trxA"
                     /locus_tag="VFR82_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU4500743.1"
                     /translation="MAGNALKVEDATWDSEVMKAPELVMVDFWAVWCGPCQMVAPIVD
                     ELANEYAGKVKVRKLNTDENPEVAGRYQVMSIPTILFFKNGQVVEKLVGARPKRQFKE
                     MIDQLLAQHAGSA"
     gene            complement(9490..>10028)
                     /locus_tag="VFR82_03765"
     CDS             complement(9490..>10028)
                     /locus_tag="VFR82_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500744.1"
                     /translation="RSVPMSVCYSLVLWTTVLLLCTGCGTLASTPDAVHPTHLQRARV
                     FLAAGDFRRAVEACRQEVQEHPSARSYLYLTYVYQALDAYVDSLAKADHWVGMEHLYL
                     NLTSGGPEELLDPPNVLARMAKEMIQAATRQQSDVTSELATRLDPSVVALVWPQQTAW
                     RKAMPERWWFGVPPEWPW"
BASE COUNT         2155 a         3055 c         2696 g         2122 t
ORIGIN      
        1 ccgcttaccc agtcccgcca ggttcaggtg gacatcactg aaaccgagca cacggtcgtt
       61 gcacccgcat ggtccactcg tgacagtccg ctcgatgctg cagaactgtt tcaactagcc
      121 cgagaactcg gcgccaccct cgacatttcg gcaccggcac aaggtccgtt tcgcatcacc
      181 gtgcgcttcc ctacgatttc ttttccctct ccaccacgac cgcatgcgat gccggaatca
      241 ggcgtccccg tcgccattca gcccccgacg gtgacggcga ctccgccaac tcgtgacatc
      301 agtcaggcta cgccggcgcg cacggaccgc cgcagttacg cacgagtgcc caccgccctg
      361 caggcaaagg ttcacagcgg gtcatccgaa tgggatggaa cggtcgtgaa tctaagtgtg
      421 ggcggagcct gcattcaaat gcccggagac ttcccgtcgg tcgcgctcca agaagcgcac
      481 gtcacggtga cgacgtccgt ggccaccttg gaactgagcg gattcatcta tcaacgcagg
      541 ttgccggacg ccatcgcaca gccgtcgctg gttcctgcct ctcatctaat tgtcgtcctg
      601 aatcgaacgc cgcaaattga agcggcagtg ctcgcctcgc tgattaccgc gacgcatgaa
      661 cgatcgctgc tgttcgcgct ggacatacgt cttgcggcag cgccgatcgg caatctcgaa
      721 gccggcaggg atttggcgtt taacctgacc gacgatgacc ggcgcgaagc cgtccggatt
      781 ccgctgaaat tacgggcccg actggaagcg agtcgcggcc cggcgccgac aggccacatc
      841 gcggcacagt tgacgaacct cagccgctca ggtgccgctc ttctcattca agcacggccg
      901 gaagatctac aagacagtct tcggattcat ttcgctcctc cccaccgagg cgaggccggt
      961 cctcttgaga ccggagctcc ggacacgatc ctcccggcac acgtggtctg gtcggcgccg
     1021 gaccgcacgg cttcaaccgc cctgcacgta cccggaacgg atttcgccgc ccgagccggg
     1081 ctccgctttg acccgcttac cccgtacgcc gagcgtgagt tgaatcgagt gatccggcaa
     1141 tacttgcgcg cccagcgcgg aagcgagacc tcttcggcag cctcgatcgt cagtatcccc
     1201 cgggagtgca gaaatgcgcg gggtcaagcc atttccattt tggacgacca tcacctgcag
     1261 ttgaccgacg ccggtcgtcc cgtcattatc atcgcccccg ggtacggaca catcgcattg
     1321 gactatgccg cactcgccta ttatctcgct gaacaccaat tccgcgtcct ccgttatgac
     1381 cccacgaatc acctgggcaa cagcgatggc gagctccaac acaccaccct gcgcaatctg
     1441 caacatgatc tggacaaggt cattgagttc gtccgggata cctggccgca agctcctgtc
     1501 ctcgtgattg caagcgatct tggggcacgg gccgccctca agtcagccgc acaaattcgc
     1561 ccgctcgacc tgctgctcct ggtcaacccc gctgttgatg tgaggcatct ccttatgact
     1621 gcccatgggc atgatctcct cgcggactat cagttcggct tgcgtcgggg tgtgtgcaat
     1681 gtgctaggac tcaatgtgaa cgtcgatcag tttgtcggcg acttgatcgc cggacgctgc
     1741 gccgatcttg actcaactct ggaggacctc cgattgatcc gatcgccgtt gtgcattgcc
     1801 acaagccccg ccgtccctgc ggcccttcct ccagccgatc ttccgcacgc attcatgacc
     1861 gctttgggcg cacacaccaa gctcgtgaac ctctccacgc cgctgaccga gcggcggttg
     1921 gatgtccacg gcgcaccgcc ggcagccttc aagcaagtcc tcatgcagat tggatcagtc
     1981 attcccgtgc agggcctttc cgcccagcag gaaatttcgg cccacccctt cgtcgcacga
     2041 cagcgacgga tcgaacagga gtacaccttc ctgaggcatg agggttcgca aatcaatcgg
     2101 gaagcgctct gcgctgccta cctgacgcaa ctgcctcaac tcgggaatct gcatgagtac
     2161 aggaaattac tggatgattt atacggtttg atgagcccgc tcgattccgg cagcgtgctc
     2221 gttgatgccg gggtcgggca aagcgatttg acgcgtgcgg cattggtgaa tcacacctat
     2281 cgggccggtc aagcgagtcc cctaggccgg cggccgccac ttatgatcgg tgtcggacgt
     2341 gctcgcgagc gactcgtaca atcccgccat gcggtgctcc tcctacagcg cgagctcgcc
     2401 tcaggatttg tcggccgtcc gtcggcgatg ccgccgctgg ccattggatg gaaccaggcc
     2461 gactggatga gctcccttcc ctttaaaacc ggctcggtaa cccgtctcgt ctgtaatctc
     2521 tcactgccct atgctccatc tccggcgatc gcgctccgag aatggcatcg aactctgcat
     2581 ccggaaggtt cgctgatcgt cacgacgttc cacccggaca ccgacctctc gccgctctac
     2641 cggcgccatc tccggcaggc caaccaggac gagttcagcg cccaagctca accactcctc
     2701 cactacttcg cccgcttacg tgaagcgatc cgccatcggc ttcttcatac gttcgacgag
     2761 gccgggctgg cttccttgct tcggcattgc ggcattgagt cctttcgcat tcttcctatt
     2821 tttgacggcc aagccctggt cgccgtggtc gggaaacaga atacctctag ctccattcgc
     2881 taggccggtg tatactccac cgctgtacaa ctttgaacat ccaattcatc tccagatcca
     2941 ttggcagcgc tatgccgaca aagacgatgc atcgttgtgc ctccctctct ctatgagcgc
     3001 acgaccgact gagatgggtt cagacgcgac gccctcgtcg atcgatcttt tggattcggt
     3061 tactcgtttt tcccatgaaa taggcaccct gtcggatctc ggagcattgg gtgaacgaat
     3121 cattcgtgaa ctgtgtcgag taagttccac gcgtcacggc gtgctctatg tgctcgatcg
     3181 cgaacacgag catttccgcc gtgcctgcat cgtcggtgtc acggccgaat cctgctgccc
     3241 cgcgatcctt gctctgacag acacgatccc caagcgcctg tcctctgccc cacggacagt
     3301 gaccggatcg tgttgtgtac aaattcgacg ggaagacgct tgggacgatg cggcggcgat
     3361 gcaaatcgag tgggccttgc cgcatgtcag ccggggccgg ctcatcgctt tctcgctttt
     3421 tggtcgtagc ctctcccaag attctgactc tccatccacc ttgtcgctcc tcaatgccct
     3481 cgctcaagcc gcgaccaacg cactcgacac gcgaatgttg ctggaagatc ttcagcggtc
     3541 acatctcttg atgaagcgca cggaccgttt gaaatctctt gaaacgatcg ccggaggatt
     3601 tgcgcacgaa atacgcaacc ccctcacctc cattaaaact ttcatccagc ttgctcccga
     3661 acgcaaagat gatccggatt tcattcgcga atttagccgc gtcgtgcttg acgatgtcta
     3721 tcgcattgaa cggcttatag aggaaatttt ggattatgcc aggtacatgg aacccaagct
     3781 gaccgaagag gacccgaacg acattgtgtc gtcctgcctg tatttcattg aagtcaaagc
     3841 ggacagccgt gggatcaaga ttgaaaaaga gttggtgtcc gatctccctc gtgtcatgct
     3901 ggaccgtcag caaatcaaac aggtgtttct gaatctgttt ctgaacgcct tggatgcgat
     3961 gagggactgc ggtggggtgc tgcgcgttcg gacgaggaat ctgatcaagc caggcggaac
     4021 gtcgtgggtg cagatcgaga cgcaggatac gggtcatggg attcaggagg ccaatctgga
     4081 acacatcttc gatccgttct tcacgacgaa gcacgagagc ggcgaacatg aaggtaccgg
     4141 cctcggcttg acaatcgtgc accagatcat cgaggaacat cacggcgaaa ttcaagttac
     4201 gagcaaggtg ggcagcggca cgacatttct cgtcaacctc ccggccattc catcatgacg
     4261 acaaaaaccg cgatcgaatc gagtctcaaa aagcgcgtgc tattggtcga tgacgaagcg
     4321 cgtgtcaggg cgtcgctgaa agcggtgctc gagccgagtt atgaagtgat ccaggcggcc
     4381 gatgcccagg aagggctcga cttattccac aaagaagcgc cgcatgtggt actgctcgac
     4441 gtgattctcc cagggacgga cggtttgtcg gttttgcaga cgttgcgaac ggagcagaac
     4501 accacgccgg tcatcatgct aacgggaaca aaatccgtta agacggcggt cgatgcgatg
     4561 aaactcggcg ccgccgatta cctctcgaag cctttcgatg ttgaagagct gcgtattgtc
     4621 gtggatcgcg cgctcaactc tcacgaactc gaacgcgagg tcaaacagct tcgagcacaa
     4681 gtcacccagc ggtacgcgtt tcacaacctg atcggaaaga gccgtccgat gcaggaaatt
     4741 tacggcaaaa tcgaacaggt cgcagacagt cgcaccaccg tgctgattac gggcgaaagc
     4801 ggcaccggca aggaattggt ggccaaggct ctccactaca acagcggccg gcgggaccgt
     4861 ccgtttgtcg ctctgaattg cgccgctctt ccggaaacct tgatcgaaag cgaactcttc
     4921 ggccacgaaa aaggctcgtt cacggatgcc acggcgcggc gtgtggggca gttcgaactg
     4981 gccaacagcg gcacactatt tctcgacgaa atcggcgatc tcagcgcgat gacccaggcg
     5041 aagctgttga gagtcctgca agaacgagaa tttactcgga tcggcggtgt gcaggccatc
     5101 aaagtggacg tgcggatcgt ggcggcaacc aacaagaacc tcgaggaact ggtccgaaaa
     5161 ggccagttcc gcgaagacct atactaccgc atcaatgtga ttgcgctgta tctcccgccc
     5221 ctgcgcgaac gcggtgaaga cgtgcccctg ctggccaaac attttctatc gaaaaggatt
     5281 gaggaggatc accggcccag tcaggagttt tccaaggagg ctattgacct cctggcacgc
     5341 tacccatggc ctggcaacgt ccgggaaatg gaaaacatca tcgaacaggc gttcatctgg
     5401 tcgaaggggt cagacgttgt cacaccggag catctaccga ctattctccg taatgacaca
     5461 agatcctcct cgcttcggga cgataccttg gccggacgcc tgtcgctgga aaaagccgtc
     5521 atggagttcg agcgtgaaat catcctggac gcgctgaaaa agaataatta tgtgcaaact
     5581 cacgccgcca gccaactcgg tatcagccgg cgaatgctga aatatcgaat ggatacgctg
     5641 ggcatcggcc gtccggacag tgaatcgcct cctgagccgg agcctattgc ccaagaataa
     5701 gaaccgtccg tccgctcggt tgatcatcct cccgccggca tctttcccta gctatggctc
     5761 taggtattca ttcatacgtg gaacaatata tggtgcctcg gcagtaaagg accacatcta
     5821 ctagaagttg gatgtataac tgcctgaaaa agatgatata ttgcttgaca ggttacgtat
     5881 tccagggtac atgagcgctt aatatgacca tccctgcact caggaacatg ccggcacaga
     5941 agcccacagt cctggtcatt gatgacgaag ccgggcctcg agacgcgttg aaagtcattc
     6001 tgcgcccgtt cttcaatatc caatcggcgg aatcggctca agcggcaatc aacgtattaa
     6061 accatcagca agtcgacctg attaccctcg atcaaaaact tcccgatcgc caggggatgg
     6121 acttgttaca ggagatcaaa caggaccacg ccaacattga ggtgatcatc atcacggggt
     6181 acggtagctt gaagtcagcc atggagggca tcagacacgg ggcagcgggg tacctgctca
     6241 agccctttaa tgtcaccgag ctaatcacgc tggtcaacca gacactcgaa aagaagcagc
     6301 ggctcgacta cctccgccgc tttctgggaa cgtccaccgg gctttggggt tccgatcggg
     6361 aatgcgcgca ggcctggcac gagatgcgtg aagggtatga ggccatcggc aaatgcagcg
     6421 ggcagagcct ctctgctcct gacgatgcaa gcaatttgct tccgatgctg tccgacctcc
     6481 tggaagcgaa agaccgccag ctcttaaatc attgcagccg cgtcagcttt tacgccacac
     6541 tgctggctaa tcggacgaat ctcactgtgg aggaacagaa atcccttgca ctcggggcgt
     6601 ttctgcatga tatcggaaag gtcgggaaag aagtctatcg atttgcagac gacgaaatcg
     6661 tcgcgacaaa caccacacat gagctgcgtt cccaccccga aataggggcg aagatggtgg
     6721 ctccgctcgg gttaccggca gcaacaggcc agatcattgc ctatcaccat gagcggtggg
     6781 acgggtcagg ttatccgcac ggcttaaagg gagaagggat tcctattctg gccagaattg
     6841 tctgtctcgc ccagacattc gatcacctca ccgccgagct tccagaccgg atagcgctcc
     6901 ccatcgacga agcgagcagc ttcattgcgg ctcgtgccgg tagttatttt gaccctcagc
     6961 tcaccgcctt gttcacgcag gttgttcagg aatgcaaggc ctcattgcca gccatggcca
     7021 ttgctaccag cccgtcgacc cgctcggacg tctaggccta ctcctcgttg ccgacgtgat
     7081 gttgtgtcga gtggagctct cgcaacccat cagccggcgc gcatgacaac tagcgcttgc
     7141 tcctggcacc ggcgatcagt tctgctgccg tctccctgat tttcttggtc accgtttctc
     7201 caccgagcat ccgtgcgatt tcctcttcgc ggcccacgcc gctgagcaac ctcaccgacg
     7261 tggtcgtcct cttgccgtct tgcccttttt ccacaatcac gtggtgctca gcttgagagg
     7321 ccacctgtgg caaatgagtg atgcaaaaga cctgatggta ggcgccaagc tttcttaacc
     7381 gggtgcccat ggcagctgcc accgctcctc cgacaccggt atcgatctca tcaaaaacca
     7441 gaactggcac ctgatccgtc tcggcgagta ccgttttcag tgcgagcatg atacgcgaga
     7501 gttctccgcc ggacgcgacg cgattgagag gacgaggcgg ttccccgaca ttgctggaca
     7561 agaggaattc cacacgatca cgaccggttg gcccgaaccg atcgagttcc tcgtctgccg
     7621 tcacaacgat gtcgaacagg gtatgtgcca tcctgactgc tgccagttcc ttcgcaacca
     7681 gggtggtcag ccgcttcgcg gcttctcgac gtttttttgt cagttgcttg gccttgtcaa
     7741 tgacccgagg ctccacctcc ttcagtcgct tcatgacacc ggacatctgg gcttcatgct
     7801 gttccagcgt ctccaattcc tgcttggcac gcttgcccgt ttccagaact gcttcggtgg
     7861 agcctccata cttcgtcttc aggcggtgaa tcaggtccaa ccggtcttca acggtggctt
     7921 gcctcgctgg atccgcttca agatcgtcgg catatcctcg tagacaagcg gtcacgtctt
     7981 tcaagtggat gatcgcttgg cggacctgag cttcgcactc ccccatgacc ggatctgttt
     8041 gggccaattc cgacacaaac cggccgaccg tggccagctg tgagagagtt ccccgctcgt
     8101 ctccctggag agcggaataa gacgattcgg caagctcacg gaggcggtga gcatgcagca
     8161 gccgttgtcg ctccattttg agccggtctt cttcgtcgac ggacaatcct gcctgctcaa
     8221 tctcctggac ttggaaccgc aataactctt ccatgcgtgc tcgctcgacc cccgcgcagc
     8281 gcagagactc gatgtgggct gtcagatctc tccattcgcg ataggcggtc tcatattgaa
     8341 ggcgcaaaga ctccgttgac ccaaaggcgt ccacggcatg aagttgtttg gtagaagcca
     8401 gcaatgattg ctgttcatgc tggccgtgga tatcaaccag catacctccg aggtcttcga
     8461 gtacccggag cggacagagg ctgccgttca catacacccg atggcgaccg gagcgggaca
     8521 ggatccgctt gagaatcagg tcagtctcac gtgggcctat gagatcctgc gtccgcaatt
     8581 gttcgagcag aggatgactg aggggcagat gaaacgatgc ttccaggtgg gcttcgtcct
     8641 ctccggaacg aatctgctcc gccgagcctc tgccgcccac caataagccg atcgcatcga
     8701 tgagcaacga tttgccggcg ccggtttcgc ctgtcagaac agtaaatcct gactgaaact
     8761 ggatgtgaag ctgttcgatg agagcaaaat tgataatccg cagctcggca agcatctggc
     8821 gagttcgtaa tctctacatc gagcggatcc tgtcttagga aacagtggga gtcattgcag
     8881 ggtagtcaca aaggctggtc agcaaggtcg cagcaagcga acaggcgaaa catatctttc
     8941 tcacccgccc atccccagct gccagagcag ctctttcccg ctctgtacgt tgagcctttg
     9001 agcaacgcga agaacgacgc tgaccgcctt ttatcacaga cctgctaagc ggagccggcg
     9061 tgctgcgcca agagctgatc aatcatctcc ttaaattgcc gtttgggtct ggccccgacc
     9121 agcttttcca cgacctggcc gttcttgaag aagagaatcg tcggaatgct catgacctga
     9181 tagcgaccgg caacttcggg attttcatca gtattgagct ttcgaacctt gaccttgccg
     9241 gcatattcat tagccagttc atcgacaatc ggagcgacca tctgacatgg accgcaccag
     9301 acagcccaaa agtcgaccat gaccaactca ggggctttca ttacttcgga atcccaagtt
     9361 gcatcttcta cctttaacgc gttacctgcc acgtcgcatc ctccttgaga atcaattcga
     9421 ctcatgcgtc tgaccaaatc atcgtatacc agccgtcttt aagtagtcaa acgtgcggcg
     9481 ccaatggcgc taccatggcc attcaggagg aacaccgaac caccatctct cgggcatggc
     9541 tttgcgccac gctgtttgct gcggccatac cagcgcaaca acggacggat cgagcctagt
     9601 agccagttca gaggtgacgt cggactgctg acgcgtcgcc gcttgaatca tctctttggc
     9661 cattcgcgcc aagacgtttg ggggatcgag aagctcttcg ggcccgcctg aggtcaagtt
     9721 caaatagaga tgctccatcc ccacccaatg atccgccttg gccagcgaat ccacataggc
     9781 gtcgagggcc tgatagacat aggtgagata gagatagctc ctggcggatg gatgctcctg
     9841 cacctcctgt cggcaggcct cgaccgctct gcgaaaatcc cccgcggcaa gaaacactct
     9901 tgcgcgttgc aaatgcgtgg gatgaaccgc atcgggcgtg gaagccaatg ttccacaccc
     9961 cgtgcagagc agcagcaccg tggtccagag gaccagagag taacagacgc tcattggaac
    10021 ggatcggt
//
LOCUS       DASTQY010000118        17337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_59691, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000118 DASTQY010000000
VERSION     DASTQY010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 17337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17337
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59691"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            373..2046
                     /gene="ilvD"
                     /locus_tag="VFR82_03770"
     CDS             373..2046
                     /gene="ilvD"
                     /locus_tag="VFR82_03770"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU4500745.1"
                     /translation="MPKEFKLNSHDLLVGPGRAPARAMLKAVGFTDEDLSRPLIGVAN
                     TWIEVMPCNFHLRRLSDRVKAGIRAAGGTPIEYNTIAVSDGISMGTEGMKASLISREV
                     IADSIELVARGHLFDGVVALSGCDKTIPGTVMALARLNVPSLMLYGGSIMPGQFQGHD
                     VTIQDVFEAVGKHASGKMTNAELKDLEDHACPGPGACGGQFTANTMAIAFEFLGMSPM
                     GRNGVPAMDKRKDDVAFECGKMVMDLLKRDLRPRQIITRKSLENAIAAVATTGGSTNA
                     VLHLLAIAYEAGIKLNIDDFDRINRRVPLLADLKPGGKFTAADLYAAGGTTLVAKRLV
                     EAGLLHANQPTVTGRTIGEEAKEATETAGQRVLRPLSDPIKPTGGLVILKGNLAPEGC
                     VVKVAGHSMMQFRGPARVFDREEDAFVAVNAGQIKSGDVVVIRYEGPSGGPGMREMLG
                     VTAAIVGAGLGDSVALLTDGRFSGATHGLMAGHVAPEAVRGGPIAAVKTGDTIVFDIP
                     KRQLNLELSAKEIKGRLKKVKQPVPRYTHGVMAKYARHVSSASDGAITT"
     gene            complement(2036..2809)
                     /locus_tag="VFR82_03775"
     CDS             complement(2036..2809)
                     /locus_tag="VFR82_03775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4500746.1"
                     /translation="MWVPFEILQNLLMGIPTIARLAERHHRTGLNADRAKAREVLDLY
                     CRFHPVAEKDILEIGPGHTLEVLEEAFASGAKSCTAVDVMKYLTVERAAQKGIDYRLY
                     DGKLLPFASRQFDCIWSHTVFEHLRYPEITVPECFRLLRPGGSLVAHIDLGDHTYYGS
                     QPPPAFHVFHCLRYPTWLWRLMRWNRSSYVNRLRKSEWKRLFEAAGFVVRAEASSANR
                     EIDRALPALPYLRKYSHDDAVTQVLTVWLEKPATICPRL"
     gene            complement(2867..3874)
                     /locus_tag="VFR82_03780"
     CDS             complement(2867..3874)
                     /locus_tag="VFR82_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU4500747.1"
                     /translation="MRVMWMVMCACCVAWTSADLQAKELSPRDIYEQTSPAVVMVMGH
                     SDGGKTGSAGTGSIIQADGLVLTNAHVVVEERTGKPYPRLSVFLKPPRVTGDSRMDLA
                     RMLRAKVVAYSTPLDLAILKLEEAPAPLPVVAFSESTHGRIGDRVVAIGHPEQGGLWT
                     LTTGVISAEMDNFNGVKGKHVFQTETGLNRGNSGGPLLDSEGRMIAVNTAIARVATDG
                     LPITSISFSLKSSVAAQWLRDQGLTGSMMGSTRAPSSQTNAASDLKVKPTPQSEPKPG
                     QVPNTSAPVAPPQPTKPRGSAEPMPARPYDLDALISDRAKAEADLEGMMSEMRGRMKG
                     R"
     gene            complement(3958..5076)
                     /locus_tag="VFR82_03785"
     CDS             complement(3958..5076)
                     /locus_tag="VFR82_03785"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU4500748.1"
                     /translation="MNVPLLDLKAQYRAIKAEVMAVMEAVCDEQGFVLGPRVAEFEAA
                     MARYVGCRHTIGCASGSDALLLALMALGVKSGDEVITVPFTFFATAGAISRLGAKPVF
                     VDIDSDSFNIDPRQIERAITPRTKAVIPVHLFGQCADMAAINGIAKARGIRVIEDACQ
                     AIGAAQAGRRAGLLSDVGCFSFFPTKNLGGFGDGGLMTTDDEGLAGTLSMLRVHGSRV
                     RYLHEAVGINSRLDALQAAVLHVKLTYLDRWTEGRRRNAERYQRLFAQAQLLDRVLLP
                     VTAPGNFHVYNQYTVRVRQRDELRNFLKEKGIGSEVYYPLPMHLQNCYHDLGHHKGSF
                     PVSERAAEEVLSLPIYAELTEDQQTYVVQMISEFYRRG"
     gene            complement(5093..6283)
                     /locus_tag="VFR82_03790"
     CDS             complement(5093..6283)
                     /locus_tag="VFR82_03790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4500749.1"
                     /translation="MDTICHIITKLELGGAQEVALYAVSHLDRTRFRPMLMAGPGGLL
                     TEEARRLPGVDLIIVPTLGRRIHAISDLAAFLHLVRLLRRLKPAIVHTHSSKAGILGR
                     WAAWCAGVPLILHTVHGFGITPAQPRWLQHLLILVERITGWITTHWVVVAAIDEGRGR
                     RWGLFDANVSVIRPGIDPRPFQQPPDPAARRRLREEFGAEPDEMLVGSVACLKPQKAP
                     EDLVAVAELVCSRRPKTKFVVIGDGALRSRIQALIDQRGLQDHVRLAGWRRDVDRAMG
                     SFDALLLTSHWEGLPRVMLEAASAGLPIVATKVGGVEEAVVHRERTRLYEPGDIAGLA
                     EGVEVILQQRRTEGTSPATVRHQLPKEFHIEEMVKQYQSLYDRLLSQRREKLASRLLS
                     PASS"
     gene            complement(6291..7619)
                     /locus_tag="VFR82_03795"
     CDS             complement(6291..7619)
                     /locus_tag="VFR82_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4500750.1"
                     /translation="MILIVGAGLAGLSTAYHLTEMPYRLYEREMEVGGLCRSYRKDGF
                     TFDYTGHLLHFRQPQIKALVERLLAGKLQQHIRKSFIYSHRTYTEYPFQVNTHGLPPE
                     VVRDCLMGFIATLTTPSAAVPSNDRSFKQWILDNLGEGMARHFMVPFNEKLWQVSLDE
                     LTSDWVSWLVPKPELKDVISGALGIKDRAFGYNPSFQYPATDGIHVLPESFLPGITGL
                     HQGWELMEVETKRRRVVFKHQVSGDSRTEYYESLVSTIPVPELMRRCSDMPAHLKEGA
                     DGLRWVSVYNLNMAVAREQVSDKHWLYFPETEYPFYRIGFPMNFSPALGRSGCSSMYV
                     EMSHRPSEQHSQEELLSRARSGMERAGILRSEDEIVVADVKDLRYAYVYFDRHRARVL
                     PTMLAELERRGIYSIGRYGAWEHTSMEDAIGQGKQLAERLQARGTQAVSA"
     gene            complement(7616..8662)
                     /locus_tag="VFR82_03800"
     CDS             complement(7616..8662)
                     /locus_tag="VFR82_03800"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEU4500751.1"
                     /translation="MSLLFLTFVLSFALALYGVPIARQAALKYGIVDAPDGRLKHQQE
                     PVPYFGGLAIYLAFLMSLAFTFEFRHDVLGIILGGTIVVMLGLIDDFGVLTPWTKLAG
                     QLLAVFVLIKSGIRIEIAALPEWVDLLLTLIWMIGLINAFNLLDIMDGLSAGIGAVSA
                     IGLFIAAWLQGDQTIAFLLAALIGSLVGFLKYNWKPARIYMGDTGAMFIGLLLGAMAM
                     IGKYPSDHPLSLLTPVFILGIPIFDTLFVMWIRYQRGLPIFWGSPDHIAIRLRHWGMP
                     VPHIVLVSYVATAVVAGIGLLLMTVGEDVAWTLCLGTVVVLLLSTFLLKRIDVRKPSS
                     SVAPAHAQEGSQAA"
     gene            complement(8722..9837)
                     /locus_tag="VFR82_03805"
     CDS             complement(8722..9837)
                     /locus_tag="VFR82_03805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4500752.1"
                     /translation="MPTKVMLLAVGLGVGGTETHILELASRIDRTKYDVTVCALKSGG
                     CVADDLRARGVRVVSLGGAGMFDFRVLYRLWKLMREERPDVLQSFLFWANLAARLLGR
                     LSKAVRVICSYHDEIVSEGRLLRLIDRLTFRWSHAVVCCSDAVRRSVSACLGAPAAGQ
                     TIIPFGVDARLFATTDRATREELGLREGGPVVGTVCRLVEPKKGLSVLLQAMVMLRDA
                     GDDRGCQLLIVGEGPARDSLESLSQQLGVSSWIRFAGSRRDVPRILPLLDLFVLPSLY
                     EGFGIAILEAMAAGKPVVATTVGGIPEFVVPGETGLLVEPGNAAALADAIACVLRDPD
                     RAKRMGASGRSRAQAGFQISTVVRRHEQVYEACLANV"
     gene            complement(9827..11209)
                     /locus_tag="VFR82_03810"
     CDS             complement(9827..11209)
                     /locus_tag="VFR82_03810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4500753.1"
                     /translation="MHIVQVNTADKAGGAESVAWQLFHAYRRSGHRSSLMVGWKRSGD
                     PDVIELTHDEFRGPWSRRCSAAATQLGSALGPLRGTGHLQKALRLIVGQPRRWYDRQL
                     GREDFCFPGTHYLGSAFTAAPDIVHCHNLHGGWLKDGGYFDLTALPVMSHMFPVVVTL
                     HDAWLLSGHCAHSFECERWKTGCGDCPDLTIYPAVKRDATAYNWQRKQGLFARSRLFV
                     ATPSRWLMDKVDASMLKPACIEQRVIPNGVDLTIFAPADKHQARAQLGLPHQTKILVF
                     AANGVKQNLWKDYRTMRAALARVAVLMKNRSVLFVGLGDGEAAETIGSVRIQFVPYEH
                     DPSAVARYYQAADLYIHAARAETFPNTVIEAAACGAPVVATAVGGIPEQVIDGRTGFL
                     VPPGDAESMARQIVRLLEQEGVRRLMGSEAESVARERFDVTQQVRAYLNWYDSILLRA
                     PDATALHHAN"
     gene            complement(11228..12136)
                     /locus_tag="VFR82_03815"
     CDS             complement(11228..12136)
                     /locus_tag="VFR82_03815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4500754.1"
                     /translation="MSEERLRVSIVTPSYNQAQFLEDAMLSVLGQTYSNVEYVVIDGG
                     SSDGSADIIQRHAGRLAYWTSEPDRGQYDAINKGFSRTTGDIMAWLNSDDRYMPWTLS
                     VVADIFSSHPEIDWLTSVHPVSWNSHGQAVAVDFTGGFSRESFVRGSNFPVKSSYGRR
                     WIQQESTFWRRSLWERAGGRLDSTLLMAADFELWARFFDHTDLIGVQALLGGFRSHGN
                     QRSVLYRDRYLEEAERVLRARGRWPLGRMENLARNLIWKVFRQYSFAQLPKYLQSLVF
                     STGVLYPARVLVWTGKEWEIITGFAV"
     gene            complement(12133..12972)
                     /locus_tag="VFR82_03820"
     CDS             complement(12133..12972)
                     /locus_tag="VFR82_03820"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEU4500755.1"
                     /translation="MITNLLKSSLQKLLAIGGMEVRRIVPVPMGRARNSLLQLLEQAR
                     SVGLSPRTVIDVGAAYGSFTRLCHGVFPGAHYLLIEPLDEYRPLLEQVTHSIPSSRYR
                     LAAACARNGGQLEINVHPDLVGSSLYREVETGTGVNGVPRSVRAVTVDAVVHEAEASG
                     PFLLKLDVQGAELDVLKGAEQILEECEFVVLEISFFKFFEDGPECADTIGYMKQRGFV
                     PYDIVGLQYRPLDGALSQADIAFVKEHGLFRRQHAYATAAQRDAQNKQMQQYLHELFA
                     GQS"
     gene            complement(12962..14035)
                     /locus_tag="VFR82_03825"
     CDS             complement(12962..14035)
                     /locus_tag="VFR82_03825"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU4500756.1"
                     /translation="MNVPGLTRFRSNLRKLWPRARPFGVILLYHRIATPPTDPQLLSV
                     TPERFASQMQHVSRYYEVVSLEELVSRMQHGRLSSRVIAVTFDDGYRDNLLTAKPILE
                     SCRVPATVFVSTGNLDSQAESWWDELEHLFLHPSLLPSSLQLNIDGRLVEWTLGDDAR
                     YRQEDFISHRSWTVLRSDDPTNRHRVYRALCRLLRPLDGQARQQTLHRLRVWAGGRQD
                     SPCGHHMLSRDEVRHLAEGKYVKVGAHTASHPVLACLPLAGQREEIGSSKKSLEDILG
                     HCVDSFAYPYGTRSDYTERTTALVKETGFSFACSNFPETVSARSDVYQLPRFVVRNWD
                     EARFVRALTSWWEGGTGEEACDH"
     gene            complement(14146..14949)
                     /locus_tag="VFR82_03830"
     CDS             complement(14146..14949)
                     /locus_tag="VFR82_03830"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4500757.1"
                     /translation="MLHPLRRWWPVTAQQMDGGEPSMIARGWDDYAKRWKPERFHVLP
                     GNTVEHLGDEWTAEDTSGGGTTYGLDDASIKDFSSLLSKQVLDEYLPPHSREALEIGP
                     GGGRFTSLLLPRTDILHAADPSAAMLKRLTDRFNGTSALRTYVTDGMTLPPLARGSLG
                     FVASFDVFVHFEPRLVYWYLRQIKPLLRPGGIGLIHYASIVSPIGWRQFEADLEGNVK
                     QRTSFCAFGTMCPQLMEQFLKALDLEVISSDVGIIPRDAIAVFRKTASS"
     gene            complement(14970..15677)
                     /locus_tag="VFR82_03835"
     CDS             complement(14970..15677)
                     /locus_tag="VFR82_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4500758.1"
                     /translation="MRWRRLVPWPPVGRVRFGSLRRLTPISRQFGLDRAERERCIDIA
                     FIEQFLVRHRADIRGRVLEIGDNRYTNKFGGSAVEASDILHVQPVGHATIVADLTHAD
                     TIASDSFDCIICTQTLQFVFDVRAAVKTLYRILRPGGVLLASCTGISQISRYDMDRWG
                     DYWRFTGASVTRLLGECWPSEAVSVETHGNVLLAVAYLHGLTVDELHPEDLQQRDEDF
                     QLVITARAVKPTRNQGE"
     gene            complement(15767..16561)
                     /locus_tag="VFR82_03840"
     CDS             complement(15767..16561)
                     /locus_tag="VFR82_03840"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4500759.1"
                     /translation="MSTHGRWDGAASLAARLTGRVALWKHRGLKLPLARNLRCLWLRR
                     TSALYPGRAVGTQVVRYYWNRFLTERRMDIRGRALEIGVTGTIRHFGEDRVTSVEAID
                     VAPSDPSVTIVADLAKADHVPSDQFDCFVNQFTIHIIYDFRAALYHSIRLLKPGGVLL
                     MNFPCRSGYPANGIPLGSGQRAHVYWWFTPRLVESVFTDLGIDEAHCQLQTYGNYFAL
                     TAYMAGMPSEELTADELDTVDPDFPLLICARIQKPSHWSTSYRPGR"
     gene            complement(16558..>17337)
                     /locus_tag="VFR82_03845"
     CDS             complement(16558..>17337)
                     /locus_tag="VFR82_03845"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEU4500760.1"
                     /translation="QLDRIRGRSFAARAGTLLPCGPYTVRINDGPNFFMQYKDIFVNR
                     IYHFDTESAAPTILDCGGNIGMSVLYYKQIYPAARITTFEPDPVVYGYLQDNMARHGM
                     DDVRLIQAAVCGREGSLTFHSDGKYASNLAPTDAVLEEGWTKREVPCVRLANYLTTTI
                     DFLKINIEGAEWEALADSGHLLRYVNEMVIEYHHLPGLPRTLHSILDLLDRQGFEYLI
                     NDFDSETNGGVQPPFRLGADSRYFLLIYAKRMRAVSEAGAR"
BASE COUNT         3779 a         5257 c         4918 g         3383 t
ORIGIN      
        1 gaaaagaagc acgggaaaac agtttgggaa gtggaagtgt ttggcgcaga cggaagcgtc
       61 accgaagtcc acatcgatgc cgcgaccggc accgtcatcg atacggaagc aaaaaaagaa
      121 aaacacgaag acaaaaagaa aaagaagtaa cgaggccgtg acaactgcca tccatcaagg
      181 cagtgtggaa gcagcacacc gaggcgcacg caggcagggt acgttgagga gttgaacgat
      241 tgagagcgaa gactgcggtg agagcgtgcc ccgggttttc aattgcctcg caattggagg
      301 gcgggtaggt gaaaactcct ctggggttgg cgggtaagaa gccgcatttt cagcagcgtg
      361 caagaggatc ttatgccgaa agagttcaag cttaacagtc acgatctcct cgtcggcccc
      421 ggccgggcac cagcccgagc catgctaaaa gccgtcggct ttacggacga agacctttcg
      481 cgtcctttga tcggcgtcgc caacacctgg atcgaagtca tgccctgcaa ctttcacctc
      541 cgtcgcctgt cggaccgggt gaaagcgggc atccgcgctg ccggcggcac gccgatcgag
      601 tacaacacga tcgccgtctc ggacggcatt tcgatgggga cggaaggcat gaaggcgtcg
      661 ctgatcagcc gtgaagtgat cgccgactcg atcgagctgg tggcacgagg ccatctgttt
      721 gacggcgttg tggcgctctc gggctgcgac aagaccattc cgggaacggt gatggcgctc
      781 gcacgcctca acgtgccgtc gctgatgctg tatggcggct ctatcatgcc gggacagttt
      841 cagggccatg acgtaacgat ccaggacgtg ttcgaagccg tcgggaaaca tgcgtccggc
      901 aagatgacca acgccgagtt gaaagatctc gaagaccacg cctgcccggg tccaggtgct
      961 tgcgggggcc agttcaccgc caacactatg gcgatcgcgt tcgaattcct cggcatgtcc
     1021 ccgatgggac gcaacggcgt cccggccatg gacaaacgga aggacgacgt ggccttcgag
     1081 tgcggcaaga tggtcatgga tctcctgaaa cgagacctgc ggcctcgaca gatcatcacg
     1141 cgcaaatcgt tggagaatgc gatcgcagcg gtggccacga ccggaggctc gacgaatgcc
     1201 gtgctgcatc ttctcgccat tgcctatgaa gccggcatca agctgaacat cgacgatttc
     1261 gacaggatca atcgcagggt gccgctcctg gccgatttga agccgggcgg aaagtttacc
     1321 gcggcggacc tgtatgcagc cggcggaacg acgttggtgg cgaagcgctt ggtcgaggcc
     1381 ggcctgctcc atgcgaacca accgaccgtg accgggcgga ccatcggaga agaggccaag
     1441 gaagcgaccg agacggcggg gcaacgagta ttgaggccgc tgtcagaccc gattaagccc
     1501 acgggcggat tggtgattct caaggggaat cttgcgccag aaggctgcgt cgtcaaagtc
     1561 gccggccatt cgatgatgca gttccgcgga ccggccagag tgttcgaccg agaagaagat
     1621 gccttcgtcg ccgtgaacgc cggtcagatt aagtcgggcg atgtcgtggt catccgctat
     1681 gagggaccgt ccggggggcc gggcatgcgg gagatgttgg gcgtcaccgc cgcgatcgtt
     1741 ggagcaggct tgggggactc cgtcgcactc ttgaccgacg ggcgattttc cggcgcgaca
     1801 cacggtctga tggccggcca tgtcgctcct gaagcggtca gagggggacc aattgccgcc
     1861 gtcaaaaccg gcgacacgat tgtcttcgat attcccaaac gtcagctgaa cctcgaactc
     1921 tcagccaaag agatcaaggg tcgtctcaag aaagtgaagc agccggttcc gcgctacacg
     1981 cacggcgtca tggccaaata cgcccggcat gtgtcctcgg cctctgatgg agccatcaca
     2041 acctaggaca aatcgttgcc ggtttctcca gccacaccgt caatacttgg gtcacggcat
     2101 catcatggct atacttccgc aagtatggaa gtgccggtag tgcgcgatcg atttcccggt
     2161 tggccgagga tgcttcggct cgtacgacga atcccgccgc ctcgaacaat cgtttccact
     2221 ccgatttcct gagccggttc acgtaagaac tgcggttcca cctcatgagc ctccacagcc
     2281 atgtcggata gcgtaggcaa tgaaagacgt ggaaggctgg cggcggttgg gacccgtagt
     2341 acgtatggtc gcccaggtcg atgtgggcga caagagagcc tcccggccgt agcaggcgga
     2401 aacactctgg caccgtgatc tccggatagc ggaggtgctc gaacaccgtg tgagaccaga
     2461 tgcagtcgaa ttgccgagag gcaaacggca acagctttcc atcatacaac cggtaatcga
     2521 ttcccttttg cgctgcccgc tccaccgtca gatatttcat cacatcgacg gccgtacaac
     2581 tcttggcacc ggacgcgaac gcctcctcca agacctccag cgtgtgtccg ggtccgattt
     2641 ccaaaatgtc cttttcggcg acagggtgga accggcaata caagtcaagc acctcgcggg
     2701 ccttcgcacg atcggcattc agaccggtgc gatgatgccg ctctgccagc ctggcgatag
     2761 tcggaatgcc catcagcaga ttttgtagga tttcgaaagg aacccacatg gtctaacgag
     2821 tggtcgattg cggatacctg gcccgacctg tacggtcagc gctcgcctat cgtcccttca
     2881 tccgtccacg catttccgac atcatccctt ccagatccgc ttccgccttg gcacggtcgc
     2941 tgatgagcgc atcaaggtcg tatggacgtg cgggcatcgg ctcggccgat cctcttggct
     3001 ttgtgggctg gggtggagct accggagctg aagtgttggg gacctgaccg ggttttggct
     3061 cggactgcgg agtaggcttc accttcagat ccgacgccgc gtttgtttgg gaggaagggg
     3121 ctcttgtgct tcccatcatt gagccggtca agccttggtc ccggagccat tgagctgcca
     3181 cactggactt gagagaaaaa ctgatgctcg tgattggaag gccgtctgtg gccacacgag
     3241 caatagccgt gttcacggcg atcatgcgcc cttcactatc caacaaaggc ccgcctgaat
     3301 ttccgcgatt taaccccgtc tcggtctgaa agacgtgttt gcctttcacc ccgttgaagt
     3361 tgtccatctc tgcgctgatc acaccggtcg tcaaagtcca cagaccaccc tgctcgggat
     3421 ggccgattgc gacgactcga tcaccgattc gaccgtgcgt ggactcgctg aacgcgacaa
     3481 ccggcaacgg cgcaggagcc tcctcgagct tcagtatcgc cagatccaat ggggtcgagt
     3541 aggccacgac cttggcgcgt agcatccgcg cgagatccat tctggagtct cccgtgacac
     3601 gcggcggctt gaggaagaca gacaaccggg gatatggctt cccggttcgt tcctcgacga
     3661 cgacgtgcgc gttcgtgagg accaacccgt cggcctggat gatcgaacct gtcccagcgc
     3721 ttccggtctt gccgccatcg gaatggccca tgaccatgac aaccgcaggc gacgtctgct
     3781 cgtaaatgtc ccgcggtgac agctctttgg cctgaagatc ggcacttgtc cacgccacac
     3841 agcacgcgca catgaccatc cacatgactc gcatgcatac ctccgtccga cgtatggttt
     3901 ggcgatgact tgcgctcgtc tgctagctac agcgccgctc gacaacgcct gcgcgcacta
     3961 gcctcgtcga tagaattcac tgatcatctg cacgacgtag gtttgctgat cttcggtcaa
     4021 ctcagcatag atggggagcg atagcacttc ctcggctgcg cgttccgaga cgggaaacga
     4081 tcctttgtga tggccgaggt cgtggtagca attctgcaga tgcatgggaa gaggataata
     4141 cacttccgag ccgatgccct tctccttcaa gaagtttctg agctcatcac gttgccggac
     4201 cctcaccgtg tattggttgt acacatggaa atttccgggc gcggtgactg gcagcagcac
     4261 gcgatcgagc aactgtgcct gtgcgaacag acgctggtag cgttcggcat tccgccggcg
     4321 gccttcagtc caccgatcga ggtacgtcag cttgacatga agcaccgcag cctgcagcgc
     4381 atccaaccgg ctgttgattc caaccgcttc gtgtaaatac cgcactctgc ttccatgaac
     4441 gcgaagcatc gagagcgttc cggccagtcc ctcatcgtcc gtcgtcatca atccgccgtc
     4501 accgaagccg cccaaattct tggtgggaaa gaagctgaaa caccccacgt cggagagcaa
     4561 accggcacga cggccagctt gcgccgcccc gatggcctga caggcgtctt caatgacacg
     4621 aatacctcgt gccttggcga ttccgttgat cgcggccata tcggcgcatt gtccgaatag
     4681 atgcaccgga atcaccgctt tggtacgtgg cgtgatcgcg cgctcgattt gccggggatc
     4741 gatattgaat gagtccgaat cgatgtcgac gaataccggc ttggcgccga gtcgcgaaat
     4801 ggctcccgca gtggcaaaga aggtaaacgg cacggtgatc acttcatccc cagacttgac
     4861 gccgagtgcc atcaacgcca acaacaatgc atcgcttccc gatgcacagc cgatcgtatg
     4921 gcggcaccca acgtatcgcg ccatggccgc ttcaaattcg gccacccttg gtcctaggac
     4981 gaagccttgc tcatcgcaca ccgcttccat caccgccatc acctcagctt tgatggcgcg
     5041 gtactgagct ttcagatcca gcaatgggac gttcacgagg ggctccaatg aattatgatg
     5101 acgcagggct caacaaacga gacgcgagct tttctcgtcg ctgcgacagc agcctatcat
     5161 acagcgattg atattgcttg accatttctt cgatgtgaaa ttccttcggg agctggtgtc
     5221 ttacggtggc gggcgacgtc ccttctgtgc gtcgctgttg cagtatgacc tcgacgccct
     5281 ccgccagacc ggcgatgtcg cccggctcgt acagccgggt acgctcgcga tgaaccaccg
     5341 cttcctcaac cccaccgacc ttggtcgcca cgatcggcaa ccctgccgac gccgcctcga
     5401 gcatgaccct gggaaggcct tcccagtgcg acgtcagcag cagcgcgtcg aagcttccca
     5461 tcgctcgatc gacatcgcgt ctccaacccg ctaagcgtac atggtcttgc aggccgcgct
     5521 gatcaatcag cgcttgaata cgggaccgca gcgctccgtc tccgatcacg acgaatttcg
     5581 ttttcggtct gcgcgagcag accagctctg cgacggccac caaatcttcg ggagcttttt
     5641 gaggtttgag acaggcgacg ctgccgacaa gcatctcatc aggctcagcg ccgaactctt
     5701 cacgtaaccg ccgccgagcc gccggatcag gcggctgttg aaacggccgt gggtcgatgc
     5761 caggcctgat gaccgataca ttggcatcaa acaggcccca ccgccgtcct cgcccttcgt
     5821 cgattgctgc gacgacgacc cagtgcgtgg tgatccagcc ggtgatccgt tcgaccaaga
     5881 taaggaggtg ctgcagccag cggggttgag ccggcgtgat accgaaaccg tggacggtat
     5941 ggaggatgag agggactccc gcgcaccaag cagcccagcg gccgaggatg ccggccttgg
     6001 aactgtgcgt gtgtacgatc gccggcttca ggcggcgcaa caaccggacc aggtgcagga
     6061 acgccgcgag gtcgctgatt gcgtggatac gacggccgag agtgggcaca atgatcaagt
     6121 ccacgcccgg cagtcggcgg gcttcctccg tcagcagtcc cccgggtccg gccatcaaca
     6181 tgggacgaaa tctggttcga tcgagatggg aaacggcata cagcgcgact tcctgcgcgc
     6241 cacctaattc gagcttcgta ataatgtgac agatcgtgtc cacgatgtgt ttaggctgaa
     6301 accgcctgcg tccctctggc ttgcagtcgt tcggccaact gtttgccctg tccgattgcg
     6361 tcctccatcg acgtatgctc ccacgctccg taccggccga tcgagtaaat cccccgccgc
     6421 tcgagttcgg ccagcatcgt cggcagcaca cgcgccctgt gccggtcgaa atagacatac
     6481 gcgtagcgca gatctttgac atcggccacg acgatctcat cctcgctccg caagatgccg
     6541 gctcgctcca tgccgcttcg tgcacgcgac aagagctcct cctgcgagtg ctgttccgac
     6601 gggcgatgcg acatttctac gtacatcgaa ctgcagccgg accgtcccag ggcgggggag
     6661 aagttcatgg gaaacccgat gcggtaaaac ggatattccg tctcgggaaa gtacagccaa
     6721 tgcttgtccg aaacctgttc acgcgctacc gccatgttga ggttatacac agacacccac
     6781 ctgaggccgt ccgcaccttc tttcagatgt gcaggcatgt ccgagcagcg gcgcatgagt
     6841 tcgggaacgg gaatcgtgga gacaagggat tcatagtact cggtacggct gtcgccgctc
     6901 acttgatgct tgaagaccac ccggcggcgc ttcgtctcga cttccatcaa ttcccaaccc
     6961 tgatgcagcc cggtgatgcc gggcagaaag gattcaggga gcacgtgaat gccgtctgtc
     7021 gctgggtatt gaaatgacgg attgtatccg aaagctctgt ctttgattcc caatgcgcca
     7081 ctgatcacat ctttgagttc cggtttcggc acgagccagg atacccagtc ggaagtgagt
     7141 tcatccaacg acacctgcca gagtttttcg ttgaacggca ccataaaatg cctggccatg
     7201 ccttcgccga gattgtctaa tatccattgc ttgaaggagc ggtcattcga aggcacggcc
     7261 gcgcttggcg tcgttagcgt ggcaataaat cccatcaaac agtcccgcac gacttcgggt
     7321 ggaaggccgt gggtattcac ctggaacgga tactccgtat aggtccgatg tgagtagatg
     7381 aatgattttc gaatgtgttg ctgaagcttg cccgctaaca aacgctcgac cagcgccttg
     7441 atctgcggtt ggcggaagtg caagagatga ccggtataat cgaaggtgaa accgtccttg
     7501 cggtaggaac gacaaagccc tcccacttcc atttcccgtt cgtagagccg gtatggcatc
     7561 tcggtgaggt gataggcggt gctgagaccg gccaggccgg cgccgacgat gagaatcatg
     7621 cggcttgcga gccttcctgc gcatgggccg gtgcaaccga ggacgaaggc ttcctgacgt
     7681 cgattctttt taacaggaaa gtcgagagca gcagcaccac gaccgtcccg aggcataagg
     7741 tccaggcgac gtcttctccg accgtcatca ggagcaggcc gattcctgcc accacagcgg
     7801 tcgccacata gctcacgagc acgatgtgcg gaaccggcat cccccaatga cgtaagcgaa
     7861 tggcaatgtg atccggactg ccccaaaaaa tcggcaagcc acgctgataa cgaatccaca
     7921 tcacaaacag cgtgtcgaaa atcggaatac cgagaatgaa taccggcgtg agaagcgaaa
     7981 gcgggtgatc actcggatat tttccgatca tggccatagc gccgagcagc agcccgatga
     8041 acatcgcgcc cgtatcaccc atatagatcc gcgccggttt ccagttgtat ttcaaaaatc
     8101 ccacgagact gccgatcagc gctgcgagca agaaggcaat cgtctggtct ccctgcagcc
     8161 acgccgcgat aaacagtccg atcgcgctga cggcgcctat cccggcggag aggccgtcca
     8221 tgatatccaa caggttgaag gcattgatca gtccgatcat ccagataagg gtgagcaaga
     8281 gatccaccca ttcgggaagc gcggcgattt caatcctgat gccgctcttg atgaggacaa
     8341 acaccgcgag aagttgacct gccaactttg tccaaggcgt cagcacgccg aaatcgtcga
     8401 tgagtcccaa catcactacg atcgttccgc ccaagataat gcccagcacg tcatggcgga
     8461 actcgaaggt gaatgccagg ctcatgagga aggccagata gatcgccaac ccgccgaagt
     8521 acggtacggg ttcctgctga tgcttgagcc gcccgtccgg cgcatccacg atgccgtact
     8581 tcaacgccgc ctgacgcgca ataggcaccc cgtagagggc gagggcgaac gacaagacga
     8641 atgtcaaaaa caacagagac atcattgtgc cttcagtacc cacgactcac actcggcagc
     8701 ggatctcatc acttcccacc tctagacgtt ggccagacag gcctcataga cctgttcatg
     8761 tcgtcggacc accgtagaaa tctgaaaccc tgcctgtgcc ctcgaccggc cgctggctcc
     8821 catccgcttg gcacgatcgg ggtcgcgtag cacgcatgca atcgcatcgg ctaacgcggc
     8881 cgcgtttcca ggttcaacga gaaggcctgt ctcccccggc acgacaaatt ccgggattcc
     8941 gccgacggtc gtggccacca ccggcttacc ggcggccatc gcttccaaaa tcgcaatgcc
     9001 gaagccctca taaagggacg gcagcacgaa tagatcgaga agcggcaaaa tacgtggaac
     9061 gtctctcctc gagccggcga accgtatcca ggaggacaca cccaactgtt ggctaagcga
     9121 ctcgagggaa tcgcgggccg gtccttcccc gacaatgagt aactggcagc ctcgatcgtc
     9181 accagcgtct ctcaacatca ccatcgcctg cagcagcacg ctcaatcctt tctttggttc
     9241 gacgagccga cacaccgtcc cgacgaccgg tccgccctcc cgcagcccga gctcctctct
     9301 ggttgcccga tccgtcgtgg caaacagacg agcatcgact ccaaacggaa tgatcgtctg
     9361 cccggcagct ggggcaccga gacaagcgga gaccgagcgg cgtacagcat cagagcagca
     9421 caccaccgca tgagaccatc gaaaggtcag ccgatcgatc aaccgcagca ggcgcccttc
     9481 gctgacaatc tcgtcatgat aggaacagat cactcgcacc gccttcgaca agcgcccgag
     9541 caaacgggcc gccaggttcg cccaaaaaag aaacgactgc agtacgtcgg gccgttcttc
     9601 gcgcatgagt ttccacaatc gatagaggac ccggaagtcg aacatgcccg ctcctccgag
     9661 tgagaccacc cgcaccccgc gtgctcgcag atcatcggcc acacatccac cagacttgag
     9721 cgcacagacg gtgacgtcat attttgtgcg gtcgattcgc gaggccagtt cgagaatatg
     9781 ggtttcagtg ccgcccacgc caagcccaac agccaatagc atgactttag ttggcatggt
     9841 gaagcgctgt ggcgtcgggc gcccgcagga gaatcgagtc ataccagttg agataggcac
     9901 gcacttgctg cgtgacgtcg aagcgttccc gcgccacgct ttccgcttcc gaccccatca
     9961 atctccggac gccctcttgc tcgagcaacc ggacgatttg ccgcgccatg gattctgcat
    10021 cgcccggagg gactagaaag ccggtccgtc cgtcaatcac ttgttccgga attccgccca
    10081 ctgccgtcgc gaccacaggt gcgccgcagg cggcggcttc aatcactgtg ttcggaaacg
    10141 tctcggcacg ggcggcgtga atgtacagat cggcggcttg atagtagcga gcgaccgccg
    10201 atggatcatg ctcatagggg acaaactgta tgcgcacgga cccgatggtt tctgccgctt
    10261 ctccgtcgcc caaaccgaca aacagcaccg accggttctt catgaggacg gctactcgtg
    10321 ccagtgctgc ccgcatcgtt cggtaatcct tccatagatt ttgtttcacg ccgttcgcgg
    10381 caaacacaag aatcttggtc tggtgcggta gacccaactg tgcccgcgcc tggtgtttgt
    10441 cggctggggc aaagatcgtc agatcgacgc cgttggggat gactcgttgc tcgatgcacg
    10501 caggcttcaa catcgacgcg tcgaccttgt ccatcagcca ccgtgacggc gtcgcgacga
    10561 agaggcggct tctcgcgaag aggccttgct tgcgttgcca gttgtaggcc gtcgcatccc
    10621 gcttgacggc cggataaatg gtcagatccg gacagtcacc gcagcctgtt ttccatcgct
    10681 cgcattcaaa cgaatgtgcg cagtgcccgc tcaacaacca ggcgtcgtgg agggtgacca
    10741 caaccggaaa catgtggctc atcaccggca aggccgtcag atcaaaatag ccgccgtctt
    10801 tgagccatcc gccgtgaaga ttgtgacagt gcacaatgtc gggcgccgct gtgaacgccg
    10861 agccgaggta gtgggttccc ggaaaacaaa aatcctcccg acccaactgt cggtcgtacc
    10921 atcggcgagg ttgtccaacg atgagccgga gagccttctg aagatgcccg gtcccccgca
    10981 gcggtcccaa tgccgacccg agctgagtag cggcagcaga acagcgccgg ctccaagggc
    11041 cgcgaaattc gtcatgggtc aattcgatca cgtccgggtc gccgctgcgt ttccacccga
    11101 ccatcaagga cgaacgatgg ccgctgcggc gataggcgtg aaagagctgc caggccaccg
    11161 actcggcccc accggccttg tcggcggtat tcacctgcac gatgtgcata cggaaataag
    11221 ataccggtta tacggcgaat ccggtaatga tttcccattc ctttcctgtc caaaccagca
    11281 cgcgtgcggg gtagaggact ccggtagaaa acacgagcga ttgcaggtat ttcggaagtt
    11341 gagcgaagga atactggcgg aacactttcc aaatcaggtt acgcgcaaga ttctccatcc
    11401 gtcctagtgg ccatcgccct cttgcgcgga gcacgcgttc agcctcctcc agataccggt
    11461 cccggtaaag gacagacctc tggttgccat gcgaccgaaa gccccccagc agggcttgga
    11521 cgccgatgag atcagtatgg tcaaagaacc ttgcccacaa ttcaaaatct gctgccataa
    11581 gcaatgtact gtccaatcga cctccggcac gctcccatag agaccttctc cagaacgtgg
    11641 attcctgctg gatccacctt ctcccatagg aacttttcac gggaaaattg cttccgcgta
    11701 cgaatgactc acggctgaag ccccccgtaa agtcgacggc caccgcctgg ccgtgcgagt
    11761 tccaactgac gggatgcacg cttgtcagcc agtcaatctc gggatgtgag gagaaaatat
    11821 ccgcgacaac ggacagcgtc cacggcatat atcgatcatc gctgttgagc caggccatga
    11881 tgtcaccggt ggtcctgcta aagcccttgt tgatggcatc gtactgtcct ctgtccggct
    11941 cgctggtcca ataggccaac cggccggcat ggcgttggat gatatcggcg ctgccgtccg
    12001 atgatccccc gtcgatgacg acatactcaa cgttggagta cgtttgcccg agcacggaga
    12061 gcatcgcgtc ttccaggaac tgcgcctggt tatacgaggg cgtgacgatg gaaacgcgga
    12121 gtcgttcttc gctcatgact gaccggcaaa aagttcgtgg aggtattgct gcatctgttt
    12181 gttctgtgcg tcccgctgtg ctgccgtggc ataggcatgc tgacggcgaa aaagtccgtg
    12241 ctccttcacg aacgcgatat ccgcctgcga caaggcgccg tcgaggggac gatattgcag
    12301 tccgacaatg tcatacggga caaatccgcg ctgcttcatg tagccgatcg tatctgcgca
    12361 ttccggacca tcttcaaaga atttgaagaa ggagatttcc agcacgacga actcgcactc
    12421 ctcgaggatt tgctcggcgc ccttgagtac atcaagctca gctccttgca cgtccaattt
    12481 aagaagaaac ggtcctgatg cctcggcctc gtgcaccacg gcgtccacgg tcaccgcccg
    12541 cacgctgcga ggtacgccat tcacacccgt tcccgtttca acctctcggt agagcgagga
    12601 gccgaccaag tcgggatgca cattgatctc caactgcccc ccattgcggg cacaggccgc
    12661 cgcaagcctg tagcggctcg aaggaattga atgcgtcacc tgttccaaca acggacggta
    12721 ttcatcgagc ggctcgatga gcagatagtg cgcaccggga aacacgccat gacaaagacg
    12781 cgtgaatgat ccgtacgcag ctcccacatc gatgaccgta cgcggcgata atcctaccga
    12841 gcgtgcctgt tcaagcaact gcagcaatga attcctcgcc cggcccatag gaacagggac
    12901 aatgcgacgc acctccattc cgccaatcgc caacagcttc tgcaaggagg acttcaatag
    12961 attagtgatc acaagcctcc tctcctgtgc cgccctccca ccacgaggtg agcgctcgga
    13021 cgaaccgtgc ttcgtcccag tttctgacga cgaagcgagg caactgatac acatctgatc
    13081 gggcactgac tgtctcagga aagttcgagc aggcaaagga aaaccctgtt tccttgacga
    13141 gtgcggttgt gcgttcggtg taatccgacc tggtcccgta tggataggcg aaactgtcaa
    13201 cacaatgtcc cagaatatct tcaagggatt tcttgctgga tccgatctcc tcgcgttgac
    13261 cagctagcgg caaacaggcg agcacgggat ggcttgccgt atgcgcaccc accttgacgt
    13321 actttccttc agcaagatgc cggacctcgt ctctggacag catatgatga ccgcatgggc
    13381 tatcctgacg tcctcctgcc cagacacgca gcctatggag ggtctgttgc cgcgcctggc
    13441 cgtcaagcgg ccgcaggaga cggcacagcg cgcgatacac ccggtgccgg ttcgtagggt
    13501 catcgctccg cagcacggtc caggaacgat gggagatgaa atcttcctgg cggtatcgag
    13561 catcgtcgcc cagcgtccat tcgaccagac gtccgtcgat gttcagctga agagaactcg
    13621 gcagtaacga gggatgaaga aacagatgct cgagctcgtc ccaccaagat tccgcctggc
    13681 tgtctaggtt tccggtcgag acaaagacgg tcgccggcac ccgacacgat tcaaggatcg
    13741 gtttggcagt caagagattg tctctgtagc cgtcgtcgaa ggtcaccgcg atcactcgtg
    13801 aagacaggcg cccgtgctgc atccggctca cgagctcctc aaggctgacc acttcgtaat
    13861 accgcgaaac atgttgcatc tggctggcga accgctctgg cgtcacactc aacagctgag
    13921 gatctgtcgg cggcgtcgca attctgtgat agagcaaaat caccccgaac ggacgagcgc
    13981 gaggccagag cttgcgtaag ttcgagcgga atctcgtcag tccgggtacg ttcatgtcgt
    14041 gatgaccgct ccctcgtgga tcgcttcacc atgacagggc cggtacagcc ccggcggcgg
    14101 gatcacgatg cgaagagcta tggcaggccg aagcaatgac ccgcgctatg acgaagcagt
    14161 ttttcgaaac accgcaatcg catctcgagg aataatgcca acatctgatg aaatcacctc
    14221 gaggtcaagg gctttcaaaa attgctccat gagctgcggg cacatggtgc cgaaagcgca
    14281 aaacgacgtc cgttgtttca cgttgccttc gagatcggct tcgaattgtc gccacccgat
    14341 cggcgacacg atcgaagcgt agtggatcaa gccgatacct ccgggtctca ggagcggctt
    14401 gatttgacgc aaataccaat agacgagcct cggctcgaaa tgcacaaaga cgtcgaaaga
    14461 ggcgacgaac ccgagacttc ctcttgcgag cggaggcaag gtcatgccgt ccgtgacata
    14521 ggtccgtaaa gcagaagttc cgttgaatct gtccgtcaga cgcttcagca tcgcggctga
    14581 aggatcggcg gcatgcagga tgtcggtcct cggcagcagc agcgatgtga acctgccgcc
    14641 gcccggcccg atttcgagcg cttcgcgact atgagggggc aggtattcgt ccaacacttg
    14701 tttgctcaag aggctggaga aatccttgat gctcgcgtca tccaagccgt acgtggtccc
    14761 tcctccgctc gtatcctctg ccgtccactc atcaccgaga tgctcgaccg tattgccggg
    14821 aaggacgtga aatcgctccg gcttccaccg ctttgcataa tcgtcccaac cgcgagcaat
    14881 catggatggc tccccaccgt ccatttgctg ggcagtgacc ggccaccagc ggcgcaacgg
    14941 atgcagcatt cgactccatc gcttcacact cactctccct ggtttctggt tggcttcacc
    15001 gcacgggcgg tgatcacaag ctggaaatct tcgtctcgct gctgcaggtc ttccggatgt
    15061 agctcatcca ccgttaaacc gtgtaaatag gcgacggcca aaagcacatt gccatgcgtc
    15121 tcaaccgaca ccgcttccga cggccagcat tcgcccaata atcgtgtgac tgacgctccc
    15181 gtaaaccgcc aatagtctcc ccaacggtcc atgtcgtacc ggctgatctg gctgatgccg
    15241 gtacaggacg ccaagagcac ccctccggga cgcagaatgc gatagagcgt cttgaccgct
    15301 gcccgaacgt caaacacgaa ctgcagggtt tgtgtgcaga tgatgcaatc aaaagaatcc
    15361 gaggcaatgg tatcggcatg agtgagatcc gcaacgatgg tggcgtgacc gaccggctgt
    15421 acatgcagga tgtcactcgc ctcgactgcc gaccctccaa atttgtttgt gtatcggtta
    15481 tctccgattt caagcacccg cccgcggatg tccgcccggt ggcgaacgag aaactgctcg
    15541 atgaacgcga tgtcgatgca acgctctcgt tctgcgcgat ccagcccgaa ctgccggctg
    15601 atcggtgtta gacgccgcag actcccaaac cgcacacgcc ctaccggcgg ccacgggacc
    15661 agacggcgcc atcgcatcag cacgcgttgt agcatcctgt gcaacatgtg tgtgactcgc
    15721 ttcgcctttg cgagtgccag agcgtcgccg tgtcgcggaa gagcaatcat cggcccggcc
    15781 ggtatgaggt cgaccagtga ctgggcttct gaatgcgtgc gcagatcagc agcggaaaat
    15841 cgggatcgac ggtatccagt tcatctgcgg tcaactcctc ggagggcatc ccggccatgt
    15901 aggccgtcag cgcaaaataa tttccgtagg tctggagttg gcagtgcgcc tcgtcgatgc
    15961 cgagatccgt gaacacggat tcgaccaggc gcggggtaaa ccaccagtac acatgcgccc
    16021 gctggccgct tcctaatgga ataccgttgg ccggatagcc gctccggcag ggaaagttca
    16081 tcaacaggac tccgccgggc ttcaacaagc ggatactatg atagagcgca gctcgaaagt
    16141 cgtagatgat atggatggtg aattgattga cgaagcagtc gaactgatcg gagggcacat
    16201 ggtccgcctt cgccaaatct gccacgatcg tgacgctcgg gtccgacggc gccacgtcga
    16261 tggcctcgac cgaagtgacc cggtcttcgc cgaaatggcg gatcgtcccc gtcacgccga
    16321 tttccaaggc tcggccgcgg atatccatgc gccgttcagt gagaaaccga ttccagtagt
    16381 aacggacgac ttgggtgccg accgcacgtc ctggatagag ggcagaggtt cggcgcagcc
    16441 acagacagcg gaggtttcgc gccaagggga gcttcagtcc gcggtgtttc cacaaagcaa
    16501 cacggcctgt gaggcgggcg gccagcgagg cggctccatc ccatcgtcca tgagtgctca
    16561 tcgcgcaccc gcttccgata cggcacgcat ccgcttggca taaatcagca ggaaataccg
    16621 gctgtcagcg cccaatcgaa acggcggctg cacgccgcca ttggtttccg aatcaaaatc
    16681 attgatgagg tactcaaagc cttgcctgtc gaggaggtcc aaaatgctgt ggagcgttct
    16741 cggcaatccc ggcaggtgat ggtattcgat gaccatctcg ttcacatacc gcagcaggtg
    16801 tccactgtca gccagcgcct cccattctgc gccttcgatg ttgattttca gaaaatcgat
    16861 cgtcgtggtc agataatttg cgaggcggac acaaggcact tcccgcttgg tccagccttc
    16921 ttccagcacc gcgtcggtcg gcgctaaatt actggcatac ttcccgtccg aatgaaacgt
    16981 cagcgatcct tctcgtccgc agaccgcagc ctgaatcaac cggacgtcgt ccattccatg
    17041 ccgtgccatg ttgtcttgca ggtagccgta cacgaccgga tccggctcga acgtggtgat
    17101 acgcgccgcc ggatagatct gtttgtaata gaggaccgac atgccgatgt ttccgccgca
    17161 atcgagaatg gtcggagcag cagactcggt gtcgaagtga tagatgcggt tgacgaagat
    17221 gtccttgtac tgcatgaaaa aattgggacc atcgttaata cgcaccgtgt agggaccgca
    17281 gggcagcaac gtcccggccc tggcagcgaa cgagcggccc cgtatccggt ccagctg
//
LOCUS       DASTQY010000119         5230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_446634, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000119 DASTQY010000000
VERSION     DASTQY010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446634"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VFR82_03850"
     CDS             <1..987
                     /locus_tag="VFR82_03850"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU4500761.1"
                     /translation="VEQPGTIRVIDQGVLMEAPFLDLRDRVWTKGNEQGLLGLAFHPE
                     HRGNGRFFVNYNRHEDGATVVSEFTRNGRSLNVSAETERILMVVPQPYLNHNGGMIAF
                     GPDGMLYIGRGDGGSKGDPQNRGQNPHEWLGKILRIDVNRGQPYAVPRDNPFATGNGR
                     PEVFAFGIRNPWRFSFDRETGMLWLADVGQYKWEEIDVVVAGGNYGWRIMEGAHCYNP
                     EEGCSPDGLIFPIAEYGHEGGRCSITGGYVYRGPSIKALRGTYVFGDYCSGELLGISA
                     DANRKGSTVPRVLMRTGLRISSFGEDQAGEIYLVDHTGGVYRLAASRSSLAP"
     gene            complement(1015..1827)
                     /locus_tag="VFR82_03855"
     CDS             complement(1015..1827)
                     /locus_tag="VFR82_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEU4500762.1"
                     /translation="MELTPFLFGLYKLVKYLLYPLTWVLLVMTMTTVLLCLPLSHRRL
                     RWARICAVSALILLVAISTPLVARPLLGTLEAWYPQPSLSTQEQYHAVVVLGGGVLER
                     GTLRPLPDLSSFSKNRTACGVDLFKQGYADKLVFTGGSGRVFKDGPRDALEMKRWAER
                     LGVPADAIVTEEQSRTTYENATETSRLLGSASILLVTSASHLPRAAALFTKQGFRVTP
                     VPCDFLMQNRPEDSWNQLDLFDVLPNDHAIEHTTVAVTEWAGMAVYWLNGKL"
     gene            1888..2370
                     /locus_tag="VFR82_03860"
     CDS             1888..2370
                     /locus_tag="VFR82_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEU4500763.1"
                     /translation="MTRSPRYWLVKSEPTAFSIDDFARAPRQTTCWDGVRNYQARNFL
                     REMAVGDQVLFYHSNAEPPAVVGSAEVVKTAYPDDTQFDRRSHHFDPASTTQEPRWDM
                     VDLKYKEKFRTAVPLDRLRQESQLKEMVLLRKGSRLSVQPVTGREWSIIMRIAGSRKG
                     "
     gene            complement(2438..2683)
                     /locus_tag="VFR82_03865"
     CDS             complement(2438..2683)
                     /locus_tag="VFR82_03865"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEU4500764.1"
                     /translation="MRIPSRVVLPFGYRISVRQLSDTEMDRRDPNADGIWDDELKTIY
                     LRKRLPVTRRRYILAHELGHAWLDWQHRHLDNGKAKT"
     gene            2860..3939
                     /locus_tag="VFR82_03870"
     CDS             2860..3939
                     /locus_tag="VFR82_03870"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEU4500765.1"
                     /translation="MIGVRRPFRVGDCFAGLALMLLMGFVVGPSLVLAAPHAPHGMVT
                     IDGVTVPEIGPMSTQVPTPPTNLGYAEKVELGKQLYFETRLSKNNSVSCAFCHNPGTG
                     FADARQFSIGAFGTAGGRQAPTVYNTGFIPLQFWDGRAGSLEEQAIGPIHNPIEMAEE
                     FRTVVPKIAKIPGYQKQFQLIFGGGASLQHIAEAIAAFERTIVSQNSAFDKHAMGEAG
                     AMNEAAVRGLALFRGKARCILCHNGPNFTDNKFHNLGVPQVGPLKEDLGRYLVSRAEK
                     DKGAFKTPTLRSVVETGPYMHDGVFKTLEEVIDFFDAGGGSNPNLSPLVKPLGLSSAE
                     KGDLIEFLKALTGAPLKVTVPKLPK"
     gene            4014..4541
                     /locus_tag="VFR82_03875"
     CDS             4014..4541
                     /locus_tag="VFR82_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HEU4500766.1"
                     /translation="MRTGCIALFGAVAWTVLAGCAEMPSGGAQPGAGKPTPAQGYAIH
                     VMAPHKFEDGTVHGPYHHYCKPVSPEVIQCLLFESTDPNALLTDIEYFVAKSVTRAHL
                     PLETWNKYYHDHEVEIATGRVQVLDMPEAQAKEIAALAAKTDGIIFHLWPEGAKAPDG
                     TVGHPQSVGHKLRTQ"
     gene            4596..>5230
                     /locus_tag="VFR82_03880"
     CDS             4596..>5230
                     /locus_tag="VFR82_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEU4500767.1"
                     /translation="MIESSFCFLPGIGAKTERALWQQGLLSWAGFLAAPAIRGIGAVR
                     KAHYDREILRAVERHVGGDARYFGVVLPSRDHWRLYEWLRTGVVYLDIETDAFGRITV
                     VGLYSRKGMRSFVRGKSLDGIVLSEELRRYDLLVTFCGTTFDVPMLLKQFPDLPLDHP
                     HMDLCAIGRRLGYRGGLKAIERRLGINRPDEICGLNGADAVRLWNRWRHRR"
BASE COUNT         1165 a         1387 c         1543 g         1135 t
ORIGIN      
        1 gtcgaacagc ctggcacaat tcgggtcatc gatcagggtg ttctcatgga agcgcccttc
       61 ctggatctgc gggaccgtgt gtggacaaaa ggcaatgagc agggcctctt aggcttggct
      121 tttcatcctg agcatcgcgg caatggccga ttctttgtga actacaaccg gcatgaggac
      181 ggtgcgacgg tcgtatcaga gttcaccagg aatggacgca gcttaaacgt atcggccgag
      241 accgaacgca ttctcatggt cgtgccgcaa ccctatctca accataacgg aggaatgatt
      301 gcgttcgggc cggatgggat gttgtacatc gggcgcggag acgggggctc taaaggcgat
      361 ccacaaaatc ggggtcagaa ccctcatgag tggcttggga agatcctgcg cattgacgtg
      421 aatcgcggtc agccttatgc ggtccctcgg gataatccct ttgcgactgg aaacggccga
      481 ccggaagtgt ttgcatttgg aatcaggaat ccctggcgat tttcgtttga tcgagaaacc
      541 gggatgctct ggttggccga tgtcgggcag tacaaatggg aagagatcga tgtcgtggtc
      601 gccggtggca attacgggtg gcgcatcatg gaaggcgcac actgttacaa cccggaggaa
      661 ggctgtagcc cagatggcct catcttcccc attgcggagt acggacacga ggggggccgc
      721 tgttcgatca ccggtggtta tgtctacagg gggccgtcca tcaaggcttt gcgggggacc
      781 tatgtttttg gggattactg tagcggagaa ctgctcggca tctccgccga tgcaaatcgc
      841 aaaggctcga ccgtcccccg ggtgctgatg cgaacgggat tgcgcatttc ttcttttggg
      901 gaagaccagg ccggagaaat ctatcttgta gaccacacag gtggggtcta ccgactcgcc
      961 gcttcacggt catcgcttgc tccctaggtc catctgcccg ctcctcaacc gtggctaaag
     1021 tttcccgttg agccaataaa ccgccatccc tgcccactct gtgacagcaa ccgtcgtatg
     1081 ttcgatcgca tgatcgttgg gaaggacatc gaacaggtcg agttgattcc aactgtcttc
     1141 gggacggttt tgcatcagaa agtcacaggg aaccggcgtg acgcgaaaac cttgctttgt
     1201 aaatagggcg gcggcgcggg gcagatggct ggctgaggtg accaacaaaa tggatgcaga
     1261 tcccaggagc cggcttgtct cagtggcatt ctcataagtc gtgcgcgact gttcttccgt
     1321 gacgatggca tcagcgggta ctcctagccg ctcagcccag cgtttcattt cgagagcgtc
     1381 tctcggcccg tccttaaaca cacgaccact gccgcctgtg aagaccagct tgtcggcgta
     1441 tccctgtttg aacaggtcca cgccgcacgc cgtccggttc ttagagaaag aagagagatc
     1501 cgggagaggg cggagggtgc ctcgctcaag cacgcctccc ccgaggacca cgactgcatg
     1561 atactgttcc tgggttgata aggagggttg gggataccag gcctccaagg tgccgagtaa
     1621 cggacgagcc acaagggggg tagaaattgc caccagtaag atcagcgcgc tgactgcgca
     1681 gatcctcgcc catcgaagcc ggcgatggct tagcggcaag cacaacaaga cggtcgtcat
     1741 cgtcatcacc agcaagaccc aggtcagcgg gtacagaagg tacttgacga gcttatagag
     1801 cccaaacaag aatggcgtga gttccattgc agcgcctcat agagaaatgt cttcaggaac
     1861 gtttatcacc cggtcgtcgc tctctacatg acacgttcgc ctcgttactg gctagtcaag
     1921 tccgaaccca cggctttttc tattgatgat tttgcacgcg ccccgcgaca gacgacgtgt
     1981 tgggatgggg tccggaatta tcaagcgaga aactttctgc gcgaaatggc cgtcggcgac
     2041 caggtgctct tttaccacag caacgcggag ccgccagctg tcgttggcag tgccgaggtg
     2101 gtgaaaacgg cgtatcccga cgatacgcaa tttgaccgac ggagccacca ctttgatccc
     2161 gcaagcacca cgcaggagcc ccgttgggat atggtcgatc tgaaatataa agagaaattc
     2221 cgcactgcgg tgcccctcga tcggctcagg caagaatctc aactcaaaga gatggtgctt
     2281 cttcgaaagg ggtcacgctt gtctgttcaa ccagtgaccg ggcgtgaatg gtcgatcatt
     2341 atgagaatag ctggcagtag gaagggatag gtatcagacc agggagtagg aacgcgtgca
     2401 agtcagtagc acggcgagcc tcaccgtcgg ccacgtgtca tgtcttagcc ttcccgttgt
     2461 cgagatgcct gtgctgccag tccagccagg catggccaag ttcatgcgcg aggatgtagc
     2521 ggcggcgggt gacgggaagc cgttttctca ggtaaatcgt tttcaactcg tcgtcccata
     2581 tgccgtcggc gttgggatcg cgacgatcca tctcggtgtc cgaaagctgc cggacagaga
     2641 tccgataccc gaacggcaag accacacgtg aagggatgcg catgaggtgc cccaatagga
     2701 ttgacgaaga tagcctacca caaatggctt ttggccttca tgcctgaacc aacatataaa
     2761 tcaatagctt atcaatatct agcagccaag actttggtcc atcgaaacat ggtactgttg
     2821 gctctgcgtt atcccacatg tgaaaaacag gaggcacgta tgatcggtgt gcgacgtccc
     2881 tttcgggtag gtgattgctt cgcgggcctt gcattgatgc tgttgatggg gttcgtagtg
     2941 ggtccctccc tcgtcctcgc cgctccacat gcaccgcacg gcatggtcac tatcgatggc
     3001 gtaacggtgc ccgaaatcgg cccgatgtcg acgcaagtgc cgacgccccc cacgaatctg
     3061 ggttacgccg agaaagtcga gttgggcaag cagctgtact ttgagacgcg cctgtccaag
     3121 aacaattccg tctcctgcgc cttttgccat aatccaggaa ccggtttcgc ggatgcacgg
     3181 cagttttcca ttggggcatt cggcaccgcc ggcggtcggc aggctcccac cgtatacaac
     3241 accggtttca ttccgctcca attttgggat ggtcgcgccg gttcactcga agaacaggcg
     3301 attggtccga ttcacaatcc gatcgaaatg gcggaggagt ttcgtactgt cgtcccgaaa
     3361 attgcgaaaa tccccggcta tcagaagcag ttccagctga tcttcggtgg cggagcgagt
     3421 ttgcaacaca ttgcggaggc tatcgcggcc ttcgaacgca cgatcgtgtc tcaaaatagt
     3481 gcctttgaca agcacgcgat gggcgaggca ggagcgatga atgaagcggc agtccggggg
     3541 ttggcactgt tcagaggcaa ggcgcgctgc atcctttgtc acaacggtcc caattttacg
     3601 gacaataagt tccataacct cggcgtcccg caggtcggcc ctttgaaaga agacttaggc
     3661 cgttacctcg tgagcagggc agaaaaggac aagggcgcat tcaaaacacc gactttgcga
     3721 agtgtggtcg agacgggacc gtatatgcat gacggggtgt tcaagacgct cgaagaagtg
     3781 atcgactttt tcgatgcggg aggcggaagc aatccaaacc tgagcccact ggtcaaacct
     3841 ttgggactct cgtcggcaga aaaaggagac ttaatcgagt ttctgaaggc cttgactgga
     3901 gcacccctga aagtcacagt gcccaaattg cccaaatagg catgaggtgc cctctcgttg
     3961 ccaaacggtg agcggccaga tcgttgtgtg tacttcaaga ttggaggttt gacatgcgaa
     4021 caggatgcat tgcactcttt ggagcggtgg cgtggaccgt cctggctggc tgtgcggaga
     4081 tgccgagcgg cggcgctcaa cctggagccg gcaaacccac acctgcccaa gggtacgcca
     4141 ttcatgtgat ggcgcctcat aaatttgagg atggtacagt ccacggaccc taccatcact
     4201 actgcaagcc ggtctctccg gaagtgattc aatgtctgtt gtttgagtcg acagatccaa
     4261 acgcgctctt gacagacatt gaatatttcg tggcaaagtc ggtcacacgt gcccatctgc
     4321 ctttggagac ctggaacaag tactatcacg atcatgaggt tgagatcgcc actggtcgtg
     4381 tgcaggtgct ggacatgccc gaagcccagg cgaaggaaat tgcagcgctc gcggccaaga
     4441 cggatggaat tatcttccat ctttggcccg aaggtgccaa ggcgccggac gggacggtgg
     4501 gacatccgca gtcggtcggt cacaaactga gaacgcaata acctcaacac ttatgcttca
     4561 ccagtgacac gcctgctaat cgaaatgaaa tcctagtgat tgaatcttct ttttgtttcc
     4621 tccccggcat tggggcaaaa acggaacgag ctctctggca gcagggtctg ctgtcatggg
     4681 ccggcttcct ggcggccccg gcgatccgcg ggataggagc tgtccgcaaa gcacactatg
     4741 accgggaaat cttgagagca gtcgagcgcc atgtcggcgg cgatgcccgc tacttcggtg
     4801 tggtcctgcc gtcccgagac cactggcgtc tctatgaatg gcttcggacc ggagttgtct
     4861 atcttgacat tgagacggat gcgtttggtc gtatcaccgt ggtgggactg tacagtcgaa
     4921 agggaatgcg ctcgttcgtt cggggaaaat cgctggacgg catcgtactt tcggaagagt
     4981 tgcggcgata cgatttgctg gtcacctttt gcggaacaac gttcgatgtt ccgatgctat
     5041 tgaaacagtt cccagatctt ccgcttgatc atcctcatat ggatctctgc gcgatcggac
     5101 gacggttggg atatcgagga ggactcaagg ctattgaacg gcgcttgggc atcaaccggc
     5161 cggacgagat ttgtggcttg aatggggcag acgccgtacg tctttggaat cgctggcggc
     5221 atcgacggga
//
LOCUS       DASTQY010000120         3525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_844991, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000120 DASTQY010000000
VERSION     DASTQY010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844991"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VFR82_03885"
     CDS             complement(<1..802)
                     /locus_tag="VFR82_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4500768.1"
                     /translation="MPPLDLRNPVAVRTVFLLSLITPVCGMAQTPVVEPPPVEKSSDW
                     HLSAYADLAYLLDFNFPENHLWRSRTTSQRVNELAPNMGFLNLTKEMTTDSRWGMEVG
                     IQGGYDAKTFGFLQDEPRVGGSDTLRHIQRANVSYLAPVGNGLSIQAGLFNSFIGYES
                     FYAKDNANYTRSWIADNTPYMMFGVNARYPVNDRLTIAGFIINGYYHLSHPNDQPSYG
                     TQIVYRPAAEVTVTETLYAGPDQNKTAFEFWRFYLNSIVERKTDDFILA"
     gene            complement(802..1494)
                     /locus_tag="VFR82_03890"
     CDS             complement(802..1494)
                     /locus_tag="VFR82_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500769.1"
                     /translation="MIQYCCLLAIAMCLLTGCATSQGLSRDSLQDVILREETSFLDTP
                     PAVAAPAPRNAPTLGFYFKPTGFVHREFEWTSRDRDAVLAWARGLSLGVGGRSTGFLT
                     PSSLKGQTLRELRATAARYGVDWVLVFDGAAAVDRYNNYKAPLLYWTILGAYLADGTH
                     SDALCLMKATVWDVKTGARLFNDQAEAITHTVGPAALVDDTREIERAKTRAMETLLQR
                     LTDRFAVLVARY"
     gene            complement(1556..2014)
                     /locus_tag="VFR82_03895"
     CDS             complement(1556..2014)
                     /locus_tag="VFR82_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500770.1"
                     /translation="MCKGLFRLLPLLMCSIGLAVVSPALAADPTAIRDILDEPSIYHL
                     KQVVLQGRVRNVQPLDPYKLPAGTTCYGAYLFYLEDETASINVAVFGLCGVPTVKDPD
                     VEEGIQVQLQATIQAPSHGGYYLSFQGLKVAGEREGVIQAVADRITPLVE"
     gene            2118..3485
                     /locus_tag="VFR82_03900"
     CDS             2118..3485
                     /locus_tag="VFR82_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU4500771.1"
                     /translation="MIMPQQLDAYVNESRVRFEDLLAQMVETPSISMDPSRAKDMGRM
                     AELSEQVLTQAGAEAEIVDTGGYPIVSGGWTTGSEHPTVTVYNHMDVQPAQEPEWRQQ
                     PFAFRNDGGIYRGRGATDDKGPALAALFGARLAIEQGVPINIRFLWELEEEIGSPHFA
                     AGLRNRAAIPRPDSVIVSDTIWIAKGKPAMPYGLRGLLGARLTLRTGSKDAHSGVTGG
                     AARNPLAELMEVAHACVNAKTGEVKIPGFYTDVVKPTREEIKSFLKSGFQVKRFKEAY
                     GLHSLRTDDPAEVTRRIWAAPTFEIHGLTGGYHGPGVKTVVPGHGELKVSMRLVPDQT
                     PEKVFSLFKQYVAKLNPNVKVEREGMLHPFKGSFEGPYVECVKRAAKAGFGNEPAFIR
                     EGGSIGAVVTMQKTWKVPILFLGLSLPEHGYHAPNEYFDWGQASGGMKAFAHYFMELA
                     KMGKR"
BASE COUNT          768 a          971 c         1077 g          709 t
ORIGIN      
        1 acgccaggat gaaatcgtca gttttccgtt ccacgatact gtttaaatag aaccgccaaa
       61 actcgaaggc tgtcttattc tggtctgggc cggcgtagag cgtttcggtc acggtgacct
      121 ctgcggcagg tctgtacacg atttgcgtgc catagctcgg ctgatcattc gggtgggaaa
      181 ggtgatagta gccgttgatg atgaagcccg cgattgtcag ccggtcgttc acgggatagc
      241 gtgcattgac tccgaacatc atatagggcg tgttgtccgc tatccacgaa cgggtgtagt
      301 tggcgttgtc tttggcatag aaggattcat atccgatgaa actgttaaac agccctgcct
      361 gtatactcaa gccatttccg acgggggcca gataggacac gttcgcccgc tggatatggc
      421 ggagggtgtc ggagcccccg acccttggct cgtcctgcag gaagccaaag gtttttgcgt
      481 cgtaaccacc ttggatgccc acttccattc cccagcgtga atcggtcgtc atctctttcg
      541 tgaggttgag aaagcccata ttgggggcca gttcgttgac ccgctgactc gtggtgcggc
      601 tgcgccacag atggttctct ggaaaattga agtccaggag gtatgcgagg tctgcatagg
      661 cgctcagatg ccagtcggaa gacttctcta cgggtggggg ttcgacgacc ggcgtttgag
      721 ccatgccgca aaccggtgta atcagcgaga gcaggaacac tgtccggacc gcaacgggat
      781 tgcgtaagtc tagaggcggc atcaatacct tgcaaccaac acggcaaacc gatcagtgag
      841 acgctgcagc aacgtttcca ttgctcgggt cttcgctcgt tcaatctccc gggtgtcgtc
      901 gactaatgcc gctgggccga cggtgtgggt gatcgcctcg gcttggtcgt tgaacagacg
      961 ggctcccgtc ttgacgtccc acaccgtcgc cttcataaga cacagcgcat cgctgtgcgt
     1021 gccatcggcc agataggcac caagaatggt ccaatacagc aagggtgctt tgtaattgtt
     1081 atagcggtcg acggcagcgg ccccatcgaa aaccaacacc cagtcgacac catagcgtgc
     1141 agcggtggcc cggagctccc gcaaggtctg acctttcaat gaggacggcg tcagaaagcc
     1201 ggtgctccga cctcccacgc caagcgatag tcctcgagcc caggccagta ccgcatcgcg
     1261 gtcgcggctc gtccattcaa attcccgatg aacgaatccc gtcggtttga aatagaaacc
     1321 gagcgtgggc gcattacgcg gcgcgggagc agcgactgcc ggcggggtgt cgagaaaact
     1381 ggtttcttcc cgcaagatca cgtcttggag agagtcacgg gaaaggcctt gactggtggc
     1441 gcagccggtg agcaggcaca tcgctatcgc gagaagacag caatactgaa tcatgtgaag
     1501 atggctcgtg tatgcgtacg ccgcaataaa gtatggggcg atgccgggag cgcgctcatt
     1561 ccaccagcgg cgtgatgcgg tcggctacgg cttggatcac gccttcccgt tctccagcga
     1621 ccttgagacc ctgaaaactc aagtaatagc cgccgtgact gggggcttga atggtcgcct
     1681 gaagttggac ctgtattcct tcttcgacgt ctggatcctt gacggtcggg acgccacaga
     1741 gcccgaagac ggcgacgttg attgacgccg tctcgtcttc caaataaaaa agataggcgc
     1801 cgtaacaggt cgtgcccgcc ggcagcttgt agggatcaag gggttgaacg ttgcggactc
     1861 tcccctggag taccacttgc ttgaggtggt agatactggg ttcatcgaga atgtcgcgga
     1921 tcgcggtggg gtctgctgct aaagcaggcg agacgacggc gagaccgatc gaacacatca
     1981 acagggggag aaggcggaag aggcctttgc acatcgatgg gcattctagc atttcttgac
     2041 ggctttccaa cgcgtgacag tgtctttgac cgcgccggat cagccgctat aatccgcagt
     2101 tcattggaga atgacacatg atcatgcctc agcaactgga cgcctacgtt aacgagagcc
     2161 gcgtgcggtt cgaagacctc ctggctcaga tggtcgagac accctccatc agcatggatc
     2221 catcacgagc gaaggacatg gggcgcatgg ccgaactctc ggagcaggtg ctgactcaag
     2281 ccggagcgga agccgagatc gtcgataccg gcgggtaccc catcgtgtcg ggggggtgga
     2341 caaccggcag cgagcatccg accgtaacgg tgtacaacca tatggatgtt caacctgccc
     2401 aagaaccgga atggcgacaa caaccgtttg cctttcgcaa tgacggcggg atctaccgcg
     2461 gacgcggagc gacggacgac aaaggcccgg cactcgccgc attgttcggc gcccgtttgg
     2521 caatcgaaca gggcgtgccg atcaatatcc ggtttctgtg ggagctcgaa gaggagatcg
     2581 gcagcccgca ttttgccgcc ggtctcagaa accgggcggc gattccgcga cccgattcgg
     2641 tcatcgtctc ggacacgatc tggatcgcca aaggcaagcc ggccatgccc tatggactgc
     2701 gtggtttgct cggcgccagg ctgaccttgc gcaccggcag caaggacgcc cattccggcg
     2761 tcacgggcgg cgccgcgaga aatccgctgg cggagctcat ggaggtcgca cacgcctgcg
     2821 tgaatgccaa aaccggcgag gtgaagattc cggggttcta cactgatgtc gtcaagccga
     2881 ccagggagga gatcaagagc tttctcaagt cgggttttca ggtcaagcgc ttcaaggagg
     2941 cttatggatt gcactcgctc cggacagacg atccagccga agtgacgcgt cggatttggg
     3001 cggcgccgac atttgagatc cacggcctga cgggcggcta tcatggtcct ggggtaaaaa
     3061 ccgtcgtccc cggacatggt gagctcaagg tcagcatgcg tctcgtaccc gaccagacac
     3121 ctgaaaaggt cttctctctg ttcaaacagt atgtggcgaa actcaatccc aatgtgaagg
     3181 ttgaacggga aggcatgctg catcccttca agggcagctt cgaaggaccg tacgtcgaat
     3241 gtgtgaagcg tgccgccaag gccgggttcg gcaacgaacc ggcgttcatc cgtgaaggtg
     3301 ggtccatcgg ggccgtcgtc acgatgcaga agacctggaa agtacccatt ctctttctcg
     3361 gactgagcct gccggaacac gggtatcacg cgccgaacga atactttgac tggggtcagg
     3421 cttcaggcgg gatgaaagcc tttgcacatt attttatgga gcttgccaag atgggaaaga
     3481 ggtaacaatc atggtgcagc atcgaactgc caacggccgc gccga
//
LOCUS       DASTQY010000121         2072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_26184, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000121 DASTQY010000000
VERSION     DASTQY010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26184"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..729)
                     /locus_tag="VFR82_03905"
     CDS             complement(1..729)
                     /locus_tag="VFR82_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500772.1"
                     /translation="MNPVWLAAIIIAGAMTMSGCVSDRKYQEALAETETAKTELATTR
                     AQKSALEQQVKTLKDLNVKFGNEAQTARDELERIQYGRDKERGSVEGRTKELEERVKQ
                     LAGQNRALRQEYEDAKRHNETLKSLVARYQKELKDQSRSAGGSLAPSMQPAPPASASA
                     PPAMAPAPSPSRPPNGTPAPGGIMNVNRASSSEMVLLLGLPKDVAERIVSNRPYKVKG
                     ELVAKNVVPKETFDMIKDRISVSP"
     gene            1007..1885
                     /locus_tag="VFR82_03910"
     CDS             1007..1885
                     /locus_tag="VFR82_03910"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU4500773.1"
                     /translation="MNEWGPTLAVILGIVEGLTEFLPVSSTGHLILAGHALGFTGEVA
                     ANAEISIQLGAILAVVVYEWPKLKTLISCAFGEQAELRHSLSGPVTVSWPDLLRRSMT
                     THPHLWFLIGLGAAFLPAGLIGFATHGWIKAHLFSPQTVAVTSVIGGIIILVVEARRT
                     RANVQQLEQVGLRDAIFIGLAQCASLIPGMSRSGSTIIGGLLVGLDRKVATEYSFFLA
                     LPTLIIATCYQMWKARSVFRPDDFVALGVGLVVSFLVAWVVIAAFLTFVKRHSLRPFA
                     YYRIVMGFVVLYVFGL"
BASE COUNT          362 a          612 c          568 g          530 t
ORIGIN      
        1 ctatgggctg acgctaatcc gatccttgat catgtcgaat gtttctttgg gcacgacatt
       61 cttggcgacg agctcaccct tgactttata cgggcgatta ctaacgatcc gttctgcaac
      121 gtctttgggc aatcccaaca gcagcaccat ttcgctggaa gatgcccggt tgacgttcat
      181 gatgcctccg ggtgccggtg ttccattcgg cggacgggag ggggacggtg caggcgccat
      241 cgctggcggc gccgacgctg aagcgggcgg agccggctgc atcgacggcg cgagggaccc
      301 tccggcagaa cgtgactggt ccttgagctc tttttgatag cgcgcgacca gagatttcag
      361 cgtttcattg tgccgcttcg catcttcata ctcctgccgc aaggcccgat tttgacctgc
      421 cagttgcttc acgcgctcct ccaactcttt ggtacggcct tcgacgctcc ctcgttcctt
      481 atcacgcccg tattggatcc gctcgagctc atcgcgagcc gtctgggctt cattgccgaa
      541 cttcacgttg agatccttga gcgtcttgac ttgctgttcc agagcactct tctgtgcgcg
      601 cgtcgtggcc aattcggttt tggcagtttc agtctcggct aaggcttctt ggtatttccg
      661 gtctgaaaca cagcctgaca tcgtcattgc tcctgcgatg atgattgccg ccaaccacac
      721 aggattcatg cgaacctccc atgaccttat cagctttgaa atggcagccg tcacgaaatg
      781 gcaaggattc ggacgcgacc ggctggactg ctcagtatcg cctcttgtgt atgccaagcc
      841 aaaaccaatg tcaatacaat taggaaccgg ccgtgagacg acgagtaccc gccacagccg
      901 gctcagtttt taccggctcg cttgacggaa tctcagcact ctgtgattat gcgagcatct
      961 gagaaacccg ctttcactgc ctgattttac tcacacggac gtctttatga acgaatgggg
     1021 ccccaccctt gccgtcatcc ttggcatcgt cgaagggttg acggaatttc ttcctgtttc
     1081 ttccaccggc catctgattc tcgccggaca tgcgctggga ttcaccggag aagtcgccgc
     1141 gaatgccgag atctcaatcc aattgggcgc cattctggcc gtggtggtgt atgaatggcc
     1201 gaaactcaaa acgctgatat cgtgcgcgtt cggggagcag gccgaattgc gccattcgtt
     1261 gagcggtcct gtcacggtct catggccgga cctgcttcgc cggtctatga ccacccatcc
     1321 gcacttgtgg tttctcatcg gattgggtgc tgcatttctt cccgccggtc tcatcggctt
     1381 tgccacgcat gggtggatca aagcgcatct cttcagcccc caaactgtgg ctgtcacgtc
     1441 ggtgatcggc ggaatcatca tcctcgtcgt tgaggcacga cgcacccggg cgaacgttca
     1501 gcaactggag caggtaggat tgcgtgatgc catattcatc gggctggcac aatgcgcctc
     1561 gctcatcccc ggcatgtcac gttcgggttc aacgatcatc ggcggcttgc tggtcgggct
     1621 cgatcggaag gtcgcgacgg aatattcgtt tttcctggcg cttcccaccc tcatcatcgc
     1681 tacctgttat caaatgtgga aagctcgcag cgtgttccgt cccgacgact tcgtcgcgct
     1741 cggtgtcggc ttggtggtgt cctttctggt cgcctgggtt gtcatcgccg cctttctgac
     1801 atttgtcaaa cgtcacagcc tccgtccctt tgcttactat cgtatcgtga tgggtttcgt
     1861 cgttttgtac gtattcggtc tgtaggctgg actgtgggcg aactcccatc gcgtcagggc
     1921 gaacatgcct gcccgcgttc cgagggtcat ccttcctgct ccattctctg ttctgccagc
     1981 gattgcgtct gatggacgca agcagggtct cggtgcgctg tggcggggac gaacaaactc
     2041 agtttcaaca gccggtcggt ggaggagtat cc
//
LOCUS       DASTQY010000122         5748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_266676, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000122 DASTQY010000000
VERSION     DASTQY010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266676"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFR82_03915"
     CDS             <1..203
                     /locus_tag="VFR82_03915"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU4500774.1"
                     /translation="VGGFPRGLAIGRITAIDREEGALFQTAELAPEVDVNHLEEVLVI
                     QTPHGESAEAVRRPDGGTKRQP"
     gene            200..703
                     /locus_tag="VFR82_03920"
     CDS             200..703
                     /locus_tag="VFR82_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500775.1"
                     /translation="MKLLLYAMLVVFLVPLQATLLPHLAIWDVRPDLGLVVAALIGVL
                     AGELNGLLVGLAIGWVLNLYSAEDLWLNLLTTGGTGLFAGLLARHVAEVTPTVLSVAL
                     LVLSLAGGLVAVFGMKHGTISDTWWMVQSVVVPQACFDAAVGAALLWLLGQRFVMQRF
                     GVVDRYS"
     gene            723..2591
                     /gene="mrdA"
                     /locus_tag="VFR82_03925"
     CDS             723..2591
                     /gene="mrdA"
                     /locus_tag="VFR82_03925"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU4500776.1"
                     /translation="MATSRLHDSELSELQRRLVVLRVGLLLVVALLALRLWHLQIREG
                     PYYRDLSENNRTRSVILEPARGLIYDRNGVLLANNVPSFSLYVSLEDVKDREALIEQL
                     SELIGIDAELVRKKLAVRGAKQLPRKIKDRLTLKEATLIESHRLDLPGVMVQVESRRN
                     YLGGVAASHVLGYVGEVSAEQLDKPEFAELHQGSIVGQYGVEKFFDQHVRGMAGQKNI
                     EVDALGHEKRTVMVDQPHAGDDLYLTIDARLQKVAEDLLGQESGAIVALDPTKGDILA
                     MASRPAFDPNVLSRELTPKQWIEIVQDEGRPLNNRASQGQYPPGSTFKVMMAAAALES
                     KTVTPASTVFCNGGYQFGRRVYHDWKATGHGSVDLRKALIHSCDVYFYTIGQRMGIDT
                     MASFAHQFGLGEETGVELPSERIGIVPSTEWKQKVKREPWLPGETISAAIGQGYVTVT
                     PLQMASLIGTVANDGVTYRPRLVRAIMDRTTGRLQELAPVPKAKLNVKPETLKLIREA
                     LAGVVTEGTGGRAKSSLVTIGGKTGTAQTAALRTGPEKDIPKKFRDHAWFVAFAPVEN
                     PKIAVAVLAEHMGHGGSAAAPLAKEVIETFVKVTAKSGGTADASLAKSQTAHAGAR"
     gene            2588..3709
                     /gene="rodA"
                     /locus_tag="VFR82_03930"
     CDS             2588..3709
                     /gene="rodA"
                     /locus_tag="VFR82_03930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEU4500777.1"
                     /translation="MIDRMMDRRGLDNFDLRFLGLIFAILGLGVLSIYSVTHDQGGPG
                     LPFFAKQMVWILLGAVAFLVMLIWDYHRIARLAYPAYAIILILLAVVLFEGKTSRGAQ
                     RWIPIGPFAFQPSEFAKLVLILVMAHYYSKAPRVGWLQRVVIPGALMLPGLLLILKQP
                     DLGSGLSFMAVYAAMLLMVGMRSQALGVILLFSLMLFPFAWEVMWGSLHDYQRQRIMA
                     FVDPNYDPGGKGYHALQSRIAIGAGELTGKGLYGGTQSQLKFLPEGHTDFVFSVFAEE
                     WGFLGVLVLLLLFVALVWLSLEIATRAKDELGALLAVGVISMLCFCVVVNIGMTAGMF
                     PIVGIPLPLMSYGGSATIMTMASLGLLLNVKRRRLSLFY"
     gene            3856..5364
                     /locus_tag="VFR82_03935"
     CDS             3856..5364
                     /locus_tag="VFR82_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEU4500778.1"
                     /translation="MGVEIAITVAREETRVAVLDNGVVTDLFGDRAKYKDFVGNIYKG
                     KVAKVLPGMQAAFVDIGLEKAAFMHVSDLSLDAEPSDTLLDSDEDDKDGDDMRPRRES
                     SKPIEQLLTEGQELMVQISKGPIGTKGPRVTTYVSLPGRYLVFMPNVEHIGVSRRIAR
                     DEERARLKDIMKRVRHPGCGYIVRTVSEGVKEEELRSDVDFLHVLWQDIKTKRDQLAA
                     PALLHTDLSLSFRVVRDLFGKKVERLWIDSRQEYDAVRDFVQRFSPEQTSRIHFYDKD
                     ESLFDHLGVEQEMARALSRKVWLKSGGYLVIDHTEAMTVIDVNTGRFVGKRDQEETIL
                     RNNLEAAKEVAYQVKLRGIGGIIIVDFIDMEREKNRDKVYHALVDAMASDKARTRISR
                     ISDLGLIEISRERVREDLLRSLSEPCRYCEGRGYTKSPTTVAYEIFREIRKIGSAAEQ
                     QRIVVGAHPSVAGLLQDEERQGLEALERECVAKVLVMPDEQLHLEQYDLVVM"
     gene            5425..>5748
                     /locus_tag="VFR82_03940"
     CDS             5425..>5748
                     /locus_tag="VFR82_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU4500779.1"
                     /translation="MQSLAASKTGSLEAWMRLRTLEGIGDHTLLALVREWHSPDAVLA
                     APADELVQRGCSPRLAEAIRRGPDRDACRRIARELDAIARKRITVRTILDPAYPSHLR
                     TIADPP"
BASE COUNT         1160 a         1511 c         1844 g         1233 t
ORIGIN      
        1 tggtcggagg ctttccacgc gggttggcga tcgggaggat tactgcgatc gaccgtgaag
       61 aaggcgcact gtttcagacg gccgaactcg ccccggaagt cgacgtcaac catctggaag
      121 aagtattggt gattcaaacg ccccacggcg agtccgcgga ggctgtccgg agaccggatg
      181 gcggaacgaa acggcaacca tgaagctctt gctctacgct atgttggtcg tgttcctcgt
      241 gccgctacag gcgacgttgc ttccccactt ggccatatgg gacgtcaggc cggatctcgg
      301 tcttgtggtc gcggccctca tcggagtatt agccggcgaa ttgaatggat tgctcgtggg
      361 gttggccatt gggtgggtgc taaatttgta ttctgcggag gatctctggt tgaatctgct
      421 cacgacagga ggcaccggat tgtttgcggg gctcttggca agacacgtgg ctgaggtgac
      481 cccgaccgtg ctttcagtgg ctctgttggt cttgtcgttg gccggaggcc tcgtcgccgt
      541 cttcggcatg aagcatggga cgatatccga tacctggtgg atggtgcagt cggtcgtcgt
      601 tccccaagcc tgctttgatg cggccgtggg tgccgcgctt ctgtggttgt tggggcagcg
      661 attcgtgatg caacggtttg gagttgtgga ccggtactcg taaagacgct ggaagacaca
      721 acatggcgac ctccaggttg cacgacagcg aattgagtga attgcagcgg cgtttagtgg
      781 tgctccgcgt ggggctcctg ctcgtcgtgg ccttattggc attgcgtttg tggcatttac
      841 agatccggga agggccgtac taccgcgatc tctctgagaa caaccgcacg cgttcggtga
      901 ttctggagcc tgcgagaggg cttatttatg accgcaacgg tgtcttgctg gcgaacaacg
      961 tgccgagctt cagtctgtat gtgtcgctgg aagacgtcaa agaccgagaa gcgctgatcg
     1021 aacagttgag cgagttgatc ggcatcgacg cggaactggt tcgaaaaaaa ctggcggtgc
     1081 gcggcgccaa gcaactgcct cgcaagatca aggaccggct gacgctcaag gaagcgacgt
     1141 tgatcgaatc ccaccggctc gatttgcccg gtgtgatggt ccaagtcgaa tctcgccgga
     1201 attatttggg cggcgtggcg gcgtcacacg tcttggggta tgtcggggaa gtgtcagccg
     1261 aacagttgga caagccggaa tttgccgaac tgcatcaggg cagcatcgtc ggacagtatg
     1321 gcgtcgaaaa attctttgac cagcacgtgc gggggatggc aggccaaaag aacattgagg
     1381 tcgatgctct gggtcacgag aagcgaacag taatggtgga tcaacctcat gcgggcgacg
     1441 atttgtatct tacgatcgac gcgcgcttgc aaaaagtggc agaagatctg ctggggcaag
     1501 aatccggagc cattgtcgca ctggacccga cgaaggggga catcctggcg atggccagcc
     1561 ggcccgcctt cgatccgaat gtgttgtcac gcgaattgac gccgaagcaa tggattgaaa
     1621 tcgtccagga cgaagggcgt ccgttgaaca acagggcgtc acaaggccaa tatcctcctg
     1681 gttcgacgtt caaggtgatg atggcggccg ccgccctcga atccaagacg gtgacgcccg
     1741 cgtcaaccgt cttctgtaat ggagggtatc agttcggtcg gcgggtctac cacgactgga
     1801 aagcgacagg acatggctcg gtcgacctac ggaaagccct catccactcg tgcgacgtct
     1861 acttctacac tatcgggcag cgcatgggca tcgataccat ggcttcgttt gcccatcagt
     1921 tcgggctggg agaagagacc ggtgtcgagc tgccgtccga acgcattggc atcgtaccct
     1981 cgacagaatg gaagcaaaag gtgaagcgtg agccatggct tcctggtgaa acgatttcgg
     2041 ccgcgatcgg gcaggggtac gtcacagtga cgccgctgca gatggcgagc ttgatcggca
     2101 cggtggccaa tgacggcgtg acctatcgtc ctcgcttggt ccgcgcgatc atggatcgca
     2161 cgacgggacg gttgcaagaa cttgcgcctg taccgaaagc caaattgaac gtgaagcccg
     2221 agacgttaaa actgatccgg gaagctctgg ctggagtcgt gaccgaagga accggcggaa
     2281 gagccaagtc ttcgctcgtc accatcgggg gaaagaccgg caccgcgcag acagccgctc
     2341 ttcgcacagg ccctgaaaaa gacattccca aaaagtttcg tgaccacgcc tggttcgtgg
     2401 cctttgcccc cgttgaaaat ccgaaaattg cggttgcagt tctggccgag cacatgggac
     2461 acggtggttc cgccgctgcc ccgctcgcca aggaagtcat cgagaccttc gtcaaggtaa
     2521 ccgcgaaatc cggcggtaca gccgatgcgt ccctggccaa gtctcaaacg gcccatgcag
     2581 gcgcgcgatg attgaccgga tgatggaccg gcgtggattg gacaacttcg atctgcgctt
     2641 tctcggtctg atctttgcca tcctcggcct tggggtgctt tccatctaca gcgtgacgca
     2701 tgatcagggc ggccctggat tgccgttttt tgccaagcag atggtgtgga tactgctggg
     2761 ggcagtcgcg tttttagtga tgctgatctg ggactatcac cgcattgcac gattagcgta
     2821 tcctgcctat gcgatcatcc tgatcctctt ggcggtcgtc ctcttcgaag gaaagaccag
     2881 ccggggagcg cagcgatgga ttccaatcgg gccgtttgcc tttcagccgt cggagtttgc
     2941 caagctggtg ctgatcctcg tcatggcgca ttattactcg aaggcgccac gagtcggctg
     3001 gctgcaacgg gtggtgattc ccggcgcgtt gatgctcccg ggactgttgc tgattttgaa
     3061 gcaacccgat ctcggcagcg gattgagttt catggccgtg tatgccgcga tgctgctcat
     3121 ggtggggatg cggtctcagg cgttgggcgt cattctccta ttttcactca tgctctttcc
     3181 gtttgcctgg gaagtcatgt ggggttcctt gcacgattat cagcggcagc gcatcatggc
     3241 gttcgtcgat ccaaactacg acccgggggg caagggctat cacgcgttgc agtcacggat
     3301 tgcgatcggc gccggagaat tgacgggcaa aggtctgtac ggcggcactc aaagtcagct
     3361 gaaatttttg cctgaagggc acacggattt cgtattttcg gtgttcgcgg aagaatgggg
     3421 gtttctcggc gtattggttc ttttgttgct gttcgtcgcg ttggtctggc tgtcgctcga
     3481 aattgcgacg cgcgcgaagg acgaactggg cgccctcttg gcggtgggcg taatctctat
     3541 gctgtgcttt tgcgtcgtgg tgaatatcgg catgaccgcg ggcatgtttc cgatcgtcgg
     3601 catccccctt ccgctcatga gttacggcgg cagcgcgacc atcatgacga tggcctcgct
     3661 cgggttattg ctgaacgtga aacggcggcg gttgagtttg ttttattgaa cggatggcgg
     3721 gccgtggtga cggcccgcct gtggtacatg tgtgcgggcg gtcagattcg gcgggaagaa
     3781 gtgcatcgga acctggggaa gcccgcgttc cacctcgcgc cgcttcgagc gatccgctga
     3841 acggaaggag tcagtatggg tgtggaaatt gcgataaccg tcgcacggga ggaaacccgt
     3901 gtggcggtgt tggacaacgg tgtggttacc gatctgttcg gggaccgggc gaagtacaag
     3961 gacttcgtcg gtaacatcta taaaggaaag gttgccaagg tcctgccagg catgcaggcg
     4021 gccttcgtgg acatcggtct ggagaaggcc gcgttcatgc acgtttccga cttatccctg
     4081 gatgcagaac ccagcgacac gctgttggat tcggacgaag acgataaaga cggggacgac
     4141 atgcgccctc gtcgggaaag cagcaagccc atcgagcaat tgctcaccga agggcaggag
     4201 ctgatggtgc aaatatcgaa ggggccgatc gggaccaaag gtcctcgtgt gacgacctat
     4261 gtgtccttgc cgggacgcta cttggtgttc atgccgaacg tggaacatat cggcgtctcc
     4321 cgcagaatcg ctcgagacga agagcgtgcg cgcttgaagg acatcatgaa gcgggtccgc
     4381 caccccggct gtggctacat cgttcggacg gtcagcgagg gggtcaagga ggaagaactg
     4441 cgatccgacg tcgattttct gcacgtgctc tggcaagaca tcaagacgaa gcgcgatcaa
     4501 ctggcggctc cggcgctttt gcatactgat ctgagcctga gcttccgggt cgtccgtgat
     4561 ttattcggca agaaagtcga gcgcctgtgg atcgattcac gccaggagta cgacgccgtc
     4621 cgcgatttcg tccagcggtt ttctcccgag cagacctcgc gcatccattt ttacgacaag
     4681 gacgagagcc tcttcgatca cctgggtgtc gagcaggaaa tggcgagggc actgagtcga
     4741 aaggtgtggc tgaaatcagg cggttacctg gtgatcgatc acacggaagc catgacggtg
     4801 atcgacgtga acacgggccg cttcgtgggc aaacgcgatc aagaggaaac gattctccgc
     4861 aacaatttgg aagcggcgaa ggaagtcgcc tatcaggtga aattgcgtgg aatcggcggg
     4921 atcatcatcg tcgacttcat cgatatggag cgggaaaaga accgggacaa ggtgtaccat
     4981 gccctcgtgg atgccatggc atccgataaa gctcgcactc ggatctcccg catttccgac
     5041 ctgggattga tcgaaatctc gcgtgaacgt gtgcgggagg atctgctgcg ctcgctctca
     5101 gaaccgtgcc ggtattgcga aggccgcggc tatacgaagt caccgaccac ggtggcctac
     5161 gaaatcttcc gcgagattcg aaagattggc agtgccgccg agcaacagcg aattgtcgtc
     5221 ggggcgcatc cctcggtggc aggtctgctc caggacgaag aacggcaggg cctggaagcg
     5281 ctcgagcggg aatgcgttgc gaaagtcctt gtcatgcccg atgaacaatt gcatttagaa
     5341 cagtacgatc ttgtggtcat gtagcagcga actccacggc cgggtacgtg tccggcggtt
     5401 gagtcccagg acacgtgagg ctacgtgcaa tctctcgcgg cttcaaaaac cggttccctc
     5461 gaagcctgga tgaggcttcg cacgctcgaa ggaataggcg atcatactct gctcgctctg
     5521 gtgcgggagt ggcacagtcc ggatgcggtt ctcgctgcac cggcggatga gctggtgcag
     5581 aggggttgca gcccacgatt ggccgaggcc atcaggcgag gaccggaccg tgatgcgtgc
     5641 cggcgcatcg cacgggaact tgatgcgatt gcgcggaagc gcatcaccgt gcggacgatt
     5701 ctggatcctg cctatccgtc gcacctgcgg acgattgctg atccgcct
//
LOCUS       DASTQY010000123         2456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1463336, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000123 DASTQY010000000
VERSION     DASTQY010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463336"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VFR82_03945"
     CDS             <1..182
                     /locus_tag="VFR82_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEU4500780.1"
                     /translation="SKKIKELTMGQVLEVVSDDEGIKKDMPAWCDTTGHHLMGLEEEQ
                     ANSGPIYKAFVKKTK"
     gene            268..1929
                     /gene="ftcD"
                     /locus_tag="VFR82_03950"
     CDS             268..1929
                     /gene="ftcD"
                     /locus_tag="VFR82_03950"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEU4500781.1"
                     /translation="MDRIVECVPNFSEGRSQATVQALIDAVASVPGVRLLDHTADRDH
                     HRSVLTVVGHPEEMVEAMFRSIRVATDLIDLRRHSGVHPRVGATDVVPFVPIKGVSMR
                     DCVQAAKRLGHRVGTELEIPVFLYERAASHRDHAALESVRRGGLEGLAFRMANDPDWS
                     PDFGPGQLHETAGAIVIGARPPLVAFNVNLLSTDVNVARSIAEKIRQSNGGIPHLKAI
                     GVELASRGMVQVAMNLTDYEAVPLHAAFTAVQSQAAERGVSIAGTEIVGLVPVAAIVE
                     TTKEALRLDHFEPPQLLETQIEAAWSRGEKAKGSRTTVEQPFRLPVPRFIEAVAAPTA
                     VPGGGAVAALVGALASALGMMGAGLSRQPATERRLKDIVERLSHLLQADGAAYRTFVE
                     ATKLPKTDQNRPTAVSSALHVATEIPLEIAEQSAKTGTLLYACSCGVKPRLRSDMQVG
                     LILAIAAAEAGLHTAGENLKIQPNHKLRRCLAVRIRRLSQNLEELKVLCYTPPPGQSG
                     KHSAQALPGKVRTRDEWKSKSSITTSRKRSKLQRKSLPEKDSFGN"
     gene            1839..2033
                     /gene="rpsU"
                     /locus_tag="VFR82_03955"
     CDS             1839..2033
                     /gene="rpsU"
                     /locus_tag="VFR82_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU4500782.1"
                     /translation="MEIKVFNNNVEKALKVAKKKLAGEGLFRELKRRRYYEKPSVRKK
                     AKQREAQRRRQKWLSKRRPE"
     gene            2086..>2456
                     /locus_tag="VFR82_03960"
     CDS             2086..>2456
                     /locus_tag="VFR82_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU4500783.1"
                     /translation="MRQDQIHEAIRILKREVRQWQEPVVGVIARTSARDPFRILISCL
                     LSLRTKDKTTSEASARLFALAYTPGRLLNLPRRTIERTIYPVGFYRTKAKAIHAICRR
                     LLDEYGGTVPDSIDELVTLPGV"
BASE COUNT          545 a          692 c          728 g          491 t
ORIGIN      
        1 cgtccaagaa aatcaaggaa ctcaccatgg ggcaggtgct cgaagtcgtc tccgacgacg
       61 aggggatcaa gaaggacatg ccggcttggt gcgacacgac cggtcatcat ctcatggggc
      121 tcgaagagga gcaggcaaat tcgggaccaa tatacaaagc gttcgtcaag aagaccaagt
      181 aatacgcgca gtggttaggc gatatgagcg gaagccggag gatgttcatc agacctccgg
      241 tttttttttg tagagtgacg acacttgatg gataggatcg tagaatgtgt gccgaatttc
      301 agcgaaggcc gcagccaggc gactgttcaa gcgctaatcg atgccgttgc gtcggttcct
      361 ggcgtgagac tgttggatca caccgcggat cgtgatcatc accgctcagt cttgactgtc
      421 gtggggcacc cggaagagat ggtggaagcg atgttccgat cgattcgcgt ggcgaccgat
      481 ctgatcgacc tgcgcaggca ttcgggcgtg catccacggg tcggcgctac cgatgtcgta
      541 ccgttcgttc ccattaaagg tgtctcgatg cgagattgcg tgcaggcggc caaacgtctt
      601 ggccaccgcg ttggaacgga actcgagatt ccggtctttc tgtacgagcg cgcggcgtcg
      661 caccgtgacc atgctgctct cgaatcggtc cgtcgcggcg gcttagaagg cctcgctttc
      721 cgcatggcta acgatccgga ctggtcaccg gacttcggtc ccggtcagct tcatgagaca
      781 gcgggtgcca tcgtcatcgg ggccagaccc cccctggttg ccttcaatgt caatctcttg
      841 tcgaccgatg tgaatgtggc gcggtccatc gccgaaaaaa tccggcagtc aaacgggggc
      901 atcccgcatc tcaaggccat cggtgtagag ctggcaagcc gtggcatggt gcaagtggcg
      961 atgaacctca cagactacga agcggtaccg cttcatgccg cgttcaccgc cgttcaatca
     1021 caggccgccg agcgaggcgt atccattgcc ggcacggaaa tcgtcgggct ggttccagtt
     1081 gcagctatcg ttgagacgac taaggaagcc cttaggctcg atcacttcga accgccgcag
     1141 cttctcgaga cacaaatcga ggccgcttgg tcccgtggag aaaaggccaa aggcagccgg
     1201 accaccgtgg aacaaccctt tcgcttgccc gtcccacggt tcatcgaagc ggttgctgcg
     1261 cccacggccg ttccaggcgg aggtgccgtc gcagcactgg tcggtgccct ggcgtcggcg
     1321 ctggggatga tgggggccgg gctgagtcgg cagccagcca cagaacgtcg tctcaaggac
     1381 atcgtcgagc ggttgagcca tctgctgcaa gccgatggtg cggcctaccg aacttttgtc
     1441 gaagcaacca aactaccgaa aaccgaccaa aatcggccga cggcggtgtc atccgctctt
     1501 cacgtcgcaa cagaaatacc cctcgagatt gcggagcagt cggcaaagac cggtacgctc
     1561 ctgtatgcct gttcttgtgg ggtgaaacct cgcctccgat cggacatgca ggtgggcctg
     1621 atcctggcga ttgccgcggc cgaggccggt cttcacacag ccggtgagaa tctaaaaatt
     1681 caaccgaatc ataagcttag aaggtgtctc gctgtcagaa tacggcggct atcgcaaaat
     1741 cttgaggaac tgaaggtgct atgctacact ccgccccctg gtcaatcggg caaacattca
     1801 gcgcaggcct tgcctgggaa agtgcggacg cgagacgaat ggaaatcaaa gtcttcaata
     1861 acaacgtcga gaaagcgctc aaagttgcaa agaaaaagct tgccggagaa ggactctttc
     1921 gggaattgaa gcggcggcgg tactacgaaa agccgagtgt gcgtaagaaa gccaaacagc
     1981 gcgaagcaca gcgacgccgt caaaagtggt tgtccaagcg ccgccccgaa tagcttccgt
     2041 tcatcagtca ccatcctcta ccccagcctt ggttcgcttc tcttcatgcg tcaggaccag
     2101 atccatgagg cgatccggat cctcaaacgg gaggtccgcc agtggcagga gccggtggtt
     2161 ggcgttattg ccagaacctc tgctcgggat ccctttcgga ttctcatctc ctgtttgttg
     2221 agcttgagaa caaaagacaa gacgacgagt gaagccagcg cccggctctt cgctttggca
     2281 tacacccccg gcaggctcct gaacctgccg cggagaacca ttgagcgcac aatttatccg
     2341 gtgggatttt accgaacgaa ggcaaaagct attcatgcaa tttgccgtcg gctcttggat
     2401 gagtatgggg gcacggtgcc cgattcgatc gatgagctcg tcactctgcc aggcgt
//
LOCUS       DASTQY010000124         4012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_65042, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000124 DASTQY010000000
VERSION     DASTQY010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65042"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFR82_03965"
     CDS             complement(<1..394)
                     /locus_tag="VFR82_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500784.1"
                     /translation="MHSPVINFIVGALLGGLVLSAAWGFFWLTIGTIGLWRRTCSWRV
                     AANSFVVGVMPLLLAWALVSVSGEVFPNMAFVVGLLVMPFILAGLALRPVPGGHRAGA
                     SMLQGIGRLIDELLGRHHACGGCDHDHGP"
     gene            complement(412..1140)
                     /locus_tag="VFR82_03970"
     CDS             complement(412..1140)
                     /locus_tag="VFR82_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4500785.1"
                     /translation="MTTPVLRVSHLRKQFGDFTAVEDVSFSIEPGEILGLLGPNGAGK
                     TTTIHMLLGLITPTAGAIEVFGMELATHREQILQQVNFSSTYISMPMALTVEENLWVV
                     ARLYGLSEIHRRVDDIVKKLEMDEFRHKVTRKLSSGQMTRLTLAKALLTEPKILFLDE
                     PTASLDPDIAHKIRALLKEERRSAGLSILYTSHNMREMEEMSDRIIFLQRGRIVAEGT
                     AREIIARFGQADLEEVFLKLARER"
     gene            complement(1159..1866)
                     /locus_tag="VFR82_03975"
     CDS             complement(1159..1866)
                     /locus_tag="VFR82_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEU4500786.1"
                     /translation="MSWTSRATIALVIVAGAMALGAVAIPLTNHPAFCASCHTIKPSY
                     DSWVKSSHKDVTCVACHVRPGIEGWLQDKAWAGTKDVAIYLFGTPTDPYNLKAQVDSQ
                     VCLGCHQHILRTSEVAPRDLPVPVKDVGLVMSHRKHMEAFGTRGKKEGCTTCHSGVVH
                     DQPIKGYPIVLPRGHVSTDNKPWHPEYPEGSYLRTRALNDCFRCHDGKAQHEGRILDR
                     KCETCHLPDKIKDFLSF"
     gene            complement(1850..2374)
                     /gene="mobB"
                     /locus_tag="VFR82_03980"
     CDS             complement(1850..2374)
                     /gene="mobB"
                     /locus_tag="VFR82_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEU4500787.1"
                     /translation="MDVPIVCFVGRSNSGKTTFIERMIPELVRAGYKIATVKHAGHGF
                     DLDTEGKDSWRHKQAGASSVVILSKGSMAMFADVSDQMNVEDVRSRFLDGSYDLIIAE
                     GWKHEGYPKIVIIRDQVGEIPISSEGLLAVVSDKPIDLQVPLFDLNDVPGVAALIMKH
                     FPLRRAPSEHELDV"
     gene            complement(2385..3665)
                     /gene="glp"
                     /locus_tag="VFR82_03985"
     CDS             complement(2385..3665)
                     /gene="glp"
                     /locus_tag="VFR82_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEU4500788.1"
                     /translation="MSGLTPLEQAQKIVLDATPVLGVEKIPLLDALGRVLGEDIIVER
                     DNPPWDNSAMDGFAVRWEDIRQEHAIEKPVTLTVIEDVPAGKMPTKTVGPGQAIRIMT
                     GAPIPKGADTVLKVEDTEPGPDQVRVFKPEAKGSNIRPQGEDVKKGERIITKGTRIRP
                     GEAGMLAILAKSFVFAHQRPRVAILSTGDELADLDERFSDEKIINSNSYGIAAAVQDA
                     GGIPFLLGIAKDTPAALKEKISHGLTADILVLSGGVSMGDYDFTKAVFRDLGAEMNFW
                     KLAIKPGQPLAFGKIQGKLAFGLPGNPVSSMVTFEQLVRPAMLKMSGHRSYGRPVVQA
                     LFQEKFSKRPDRRHFLRGVLTREDGAFKVRTTGDQGSGILTSMVKANCLIDVPVEIER
                     LNPGDVVSVQLLSGESWANYAESSQSSPHRLSCC"
     gene            complement(3692..>4012)
                     /locus_tag="VFR82_03990"
     CDS             complement(3692..>4012)
                     /locus_tag="VFR82_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEU4500789.1"
                     /translation="FCKDSEVPIIGIVENMSGLECPHCHQHIDVFRKGGGEASAQDMG
                     VPFLGRIPLDPDVVIQSDAGEPFAMFCSDTPTAEAYHEIANKVETFCKKSGSLVNVAP
                     RQHR"
BASE COUNT          838 a         1205 c         1075 g          894 t
ORIGIN      
        1 tcggaccatg atcatggtcg cacccaccgc atgcatgatg cctcccgagc aactcgtcga
       61 tcagacggcc tatgccttgg agcattgagg cgccggctcg atgccctccg ggaacgggac
      121 gcaaagcgag tcccgcaagg atgaacggca tgacgagcaa gccaacgaca aacgccatgt
      181 tgggaaagac ctcgcctgag actgagacga gggcccatgc aagcagcaaa ggcatgactc
      241 caacgacgaa actattggcg gcgacgcgcc aactgcaggt ccgtcgccac agtcctatgg
      301 tccctatggt gagccagaaa aacccccatg cggcactcaa gaccaggccc cccaggagcg
      361 cgccgactat gaaattgatc acgggactgt gcatcctgcc tcctcgggcg tttaccgttc
      421 tctcgcgagc ttgagaaaca cctcttcaag atcggcttgg ccgaaacgtg caatgatctc
      481 tcgagccgtt ccctcggcga cgattctgcc tcgctgaagg aagatgatcc ggtccgacat
      541 ttcctccatt tcacgcatgt tgtgcgacgt atacagaata ctcaatcccg ccgaccgacg
      601 ttcttctttt aggagtgcac ggattttgtg ggcgatgtcc ggatcgaggc tggccgtcgg
      661 ttcgtcgaga aaaagaatct ttggttccgt gagcagcgcc ttggccagcg ttagtcgcgt
      721 catctgaccg gaagaaagtt ttctcgtcac tttatgacga aattcatcca tctccagctt
      781 cttgacgatg tcatccactc gccgatggat ttccgagagc ccatataatc gggccacgac
      841 ccataagttc tcctccaccg tcagtgccat cggcatcgaa atgtaggtcg acgaaaaatt
      901 gacctgctgg agaatctgtt ctcgatgggt ggccaactcc atcccaaaca cctcgatcgc
      961 ccctgcggtc ggcgtgatga ggcccagcaa catgtggatc gtggtcgtct tgcctgcgcc
     1021 gttcggcccc agcaatccca ggatctctcc aggctcgatg gaaaaggaga cgtcctccac
     1081 cgcggtgaaa tcaccgaatt gtttgcgcaa atgactgact cggagcacgg gtgtcgtcat
     1141 ggttctatcg gtactggttc agaaagacaa aaagtctttt attttgtccg gaaggtggca
     1201 ggtctcgcat tttcggtcca aaattctccc ctcgtgttgg gccttcccgt catggcaacg
     1261 gaagcaatcg ttgagagccc gcgtccgaag atacgaccct tccggatact ccggatgcca
     1321 cggcttgttg tctgtggaaa catgtccacg cggcaagacg atggggtatc ctttgatcgg
     1381 ctgatcgtgt acgactcccg aatgacaggt cgtgcaaccc tctttcttgc cgcgggtgcc
     1441 gaatgcttcc atatgcttac ggtgactcat cacaagacct acatctttca cagggaccgg
     1501 aagatcgcgg ggtgcgactt ccgaggtgcg aaggatatgt tgatgacaac ccaggcacac
     1561 ctgtgaatca acctgagctt tcaaattata gggatcggtc ggtgtgccga acaggtaaat
     1621 ggcgacgtct ttcgtccccg cccacgcctt gtcctgtagc cagccctcaa tccccggcct
     1681 cacatggcat gcgacgcacg tcacgtcctt gtgcgaggac ttcacccaac tgtcgtagga
     1741 cggcttgatc gtgtggcaac tggcacaaaa tgccggatgg ttcgtcaacg gaatagcgac
     1801 tgctccgagc gccattgcgc cggccacgat caccagggcg atggtggctc tagacgtcca
     1861 gctcatgctc cgaaggagcg cgtcgcagcg ggaaatgctt catgatgagt gcggctacgc
     1921 cgggcacgtc gttgagatcg aacaacggga cttgcaagtc gataggctta tccgacacca
     1981 cggccagcag gccttcggag gagattggaa tttcaccgac ttgatcgcga atgatgacga
     2041 ttttgggata tccttcgtgc ttccaccctt cggcaataat caaatcatag gacccgtcca
     2101 aaaaccggct gcgcacgtcc tccacgttca tctgatcaga aacatccgcg aacatggcca
     2161 tgctgccttt cgacaggatg acgacgctgc tcgcgccggc ctgtttgtgc cgccagctgt
     2221 ccttgccctc ggtatccaag tcgaatccgt ggccggcatg cttgacggtc gcgatcttgt
     2281 atcccgcccg tacgagctct ggaatcatgc gttcgataaa ggtcgtcttg ccgctgttcg
     2341 accttcccac gaagcagacg atcgggacgt ccatcgaaaa cctctcagca gcaggacagc
     2401 cggtgagggc tgctctgact cgattcggca taattcgccc agctttcccc gctgagcagt
     2461 tgaacggaaa ccacatcgcc tggattgagc cgttcgattt cgacgggcac atcgatcaag
     2521 cagttggcct tgaccatcga agtcaaaatc ccggaccctt gatcgcccgt tgttcgtact
     2581 ttgaatgcac cgtcttcccg cgtgagaacg ccgcgcagaa agtggcgccg atctggacgt
     2641 ttagaaaact tttcctggaa aagtgcctgc accaccggcc ggccgtaact ccgatggccg
     2701 ctcattttga gcatcgccgg ccgtaccaac tgctcgaagg tgaccatgga agagaccgga
     2761 ttccccggca gcccgaatgc gagcttgccc tgtatttttc caaaggcgag cggctgtcct
     2821 ggcttgatgg ccagcttcca aaaattcatc tctgctccca ggtcgcggaa caccgctttc
     2881 gtgaagtcgt aatctcccat cgagactcca ccggaaagca cgaggatgtc ggcggtcaaa
     2941 ccgtgcgaaa ttttctcttt taatgcggcg ggagtgtcct ttgcaattcc cagtaggaac
     3001 ggaattccgc ccgcatcctg gaccgcggca gcgatgccat agctgttgga gttgatgatc
     3061 ttctcatcgc tgaaacgttc gtctaggtcg gccagctcat cacccgtcga aaggatcgcc
     3121 actcgggggc gctggtgggc gaaaacaaac gatttggcga gaatagccag catccctgcc
     3181 tcaccggggc ggattcgagt gcccttggta atgatccgct cgcccttttt gacatcctct
     3241 ccctgggggc gaatattcga tccttttgct tcaggcttga agacgcgtac ctgatcgggc
     3301 ccaggctcgg tgtcctcaac cttcaaaacc gtgtccgccc ccttcgggat cggcgccccg
     3361 gtcatgatcc ggatcgcttg gcctggccct accgttttgg tcggcatctt cccggcaggc
     3421 acgtcttcaa tcaccgtcaa ggtgacgggc ttttcaatgg cgtgctcctg gcggatatcc
     3481 tcccagcgca ccgcgaatcc atccatggcg ctattatccc aaggcgggtt gtcgcgttcg
     3541 acgatgatgt cctcgcccag cactcgtccc agggcatcca acagcgggat cttttccaca
     3601 cccagtacgg gtgtcgcatc caacacgatt ttttgggctt gttcgagtgg agttaatcct
     3661 gacatgacat cctgactctt cattgctccg cttaccggtg ctgccgtgga gccacgttga
     3721 ccagcgatcc gctcttctta cagaacgttt ccaccttgtt ggcgatctcg tgataggcct
     3781 ctgcggtggg ggtatcggaa cagaacatgg caaacggctc tccggcatcc gactggatga
     3841 ccacatcggg gtcgagcggg atgcgcccca gaaaaggcac gcccatatcc tgcgccgagg
     3901 cctcgcctcc gccttttcta aacacgtcga tgtgttgatg gcaatgagga cattccaggc
     3961 cgctcatgtt ctctacgata ccgataatcg gcacttcgct gtctttgcaa aa
//
LOCUS       DASTQY010000125         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_41919, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000125 DASTQY010000000
VERSION     DASTQY010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41919"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="VFR82_03995"
     CDS             complement(<1..104)
                     /locus_tag="VFR82_03995"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU4500790.1"
                     /translation="MEIGFIGLGKMGLNMVTRLQQAGHHVTAYDRSPEA"
     gene            complement(186..971)
                     /locus_tag="VFR82_04000"
     CDS             complement(186..971)
                     /locus_tag="VFR82_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500791.1"
                     /translation="MDAEGTNTLPTKEELQSELVPLPELPEPPEPPNEPAYEEIVGLA
                     LRHAKGRRGGDLVSVLLVGSGARRAVTTHSDIDLVVLVKGQADGHEIIRVADRMADIR
                     YWGHKDVEDELAYSARLPSLLRKARILHDHDAIGAKLIERASHRFRQGPPPVNMNEQI
                     RLKAECFHWLGKAQDFADKPATAQYLLTIFFEDFVTAFFRLRGLWLTAPVDVPRFISS
                     RDGVLGELAGHFLVAPTLPERLNCGRDMANLLFKDVPNPARID"
     gene            complement(974..1195)
                     /locus_tag="VFR82_04005"
     CDS             complement(974..1195)
                     /locus_tag="VFR82_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500792.1"
                     /translation="MDAVTFAQISRILELTDSLGLNREWVEIPLSPARPGLVKRLTNG
                     KLEIIVDADRPFEEWLRSLPAQIQSAQGT"
     gene            complement(1276..>2151)
                     /locus_tag="VFR82_04010"
     CDS             complement(1276..>2151)
                     /locus_tag="VFR82_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEU4500793.1"
                     /translation="ALFVPSGTMANQLAIRIQTQPGQEVIAESKAHIVRYEQGAAGAL
                     AGVQLHWVTGDRGIMGPEHIEAAIRPKDQHSIQTALICLENTHNAGGGTIYPLSTIER
                     IRAVASAHGVPMHLDGARLFNAVAATTLPPAAYAQHFETVSVCLSKGLGAPAGSLLLT
                     NDPALIDKARRYRRMYGGAMRQAGILAAAGIYALDHHIGRLKDDHDNAKRLARKLQQI
                     PAITINPQHVESNIVIFDIIGHRLSPSELVAALRRDHVLINAVGGTSFRAVTHLDVST
                     RQIDAAAEVFARILG"
BASE COUNT          417 a          613 c          671 g          450 t
ORIGIN      
        1 gcttcgggag agcggtcgta ggccgtcacg tgatgcccgg cctgctgcaa gcgggtcacc
       61 atgttcaagc ccatctttcc aagaccgatg aatccaattt ccatgacaca ccttccacag
      121 gaagcgagtt gtgatcggcg caactttcta tgataagacg cggagtagta gcgaagcgga
      181 ccctctcagt cgatgcgggc cggattaggc acgtccttaa acaagagatt ggccatgtct
      241 cgtccgcaat tcaggcgctc gggcaacgtc ggggccacga gaaagtgacc cgccagctcg
      301 ccgaggacgc cgtcgcgtga ggagatgaac cgcgggacgt cgaccggtgc ggtcagccag
      361 aggccgcgca gacggaagaa cgcagtgaca aaatcttcga aaaagatcgt caagaggtac
      421 tgtgccgttg cgggcttgtc ggcgaagtct tgcgccttgc ctagccagtg gaaacattcc
      481 gccttcaacc ggatttgttc gttcatgttc acgggtgggg ggccctgcct gaaacggtgg
      541 ctggcccgtt cgatcagttt ggctccgatt gcatcgtgat catgaagaat gcgggccttg
      601 cgcagcaacg agggtaagcg ggctgaatag gccagctcat cttcgacgtc tttgtggccc
      661 cagtaccgga tgtccgccat gcgatcggcg acgcggatga tttcatggcc gtccgcctga
      721 cctttcacga gcacgaccaa atcaatgtca ctgtgcgtgg tcacggcccg cctcgcacct
      781 gacccgacca gcagaacact cacgaggtcg ccgccccggc gacctttcgc gtggcgcagc
      841 gcgaggccta cgatctcttc ataggctggt tcattcggcg gctcaggtgg ctcaggcagt
      901 tccggcagcg gaaccagttc ggactgcagt tcttcctttg ttggaagagt attcgtaccc
      961 tcggcatcca tgactacgtt ccttgcgccg actggatttg tgccggcagg gatctcagcc
     1021 actcctcaaa ggggcgatcc gcgtcgacaa tgatttccag tttcccgttg gtgagtcgct
     1081 tgaccagccc cggacgggcg ggagacaacg ggatttcgac ccattcgcga ttgagcccga
     1141 gcgagtcggt caactctaaa atccgactga tctgtgcaaa tgtgacagcg tccatcagat
     1201 ggtgtctcca cggcttgtgc cattctagca ggatgcctga aaaatctgcc agggtgcaat
     1261 cgtatgagga ggcagtcagc cgagaatccg cgcaaagacc tcggccgctg cgtcgatctg
     1321 cctggttgaa acatcgagat gggtgacggc ccggaaactc gtgccaccaa cggcgttgat
     1381 cagcacgtgg tcccttcgta atgccgccac aagctcggaa ggagagagcc ggtgtccgat
     1441 gatatcgaag atgacgatgt tgctctcgac atgctgcgga ttgatcgtga ttgctggaat
     1501 ctgctggagt tttctggcca ggcgcttcgc gttatcgtgg tcgtccttca gtcgcccgat
     1561 atgatgatcg agcgcataga tgccggccgc ggcgaggatg ccggcctgcc gcatggcgcc
     1621 gccgtacatg cggcgatacc gtctggcttt gtcgatcaac gcgggatcgt tcgtaaggag
     1681 cagagagccg gcgggtgctc ccaacccttt cgagaggcag acggacacgg tttcaaagtg
     1741 ctgcgcgtag gccgccggcg gcaaagtcgt ggcagcgacg gcgttgaaca gtctcgcgcc
     1801 gtccaaatgc atggggacac cgtgtgcgga cgcgacagcc cggatgcgtt cgatcgtcga
     1861 cagcggatag atggtcccgc cgccggcatt gtgcgtgttc tccagacaga tgagcgccgt
     1921 ttgaatgctg tgctggtctt tgggacggat tgcggcttcg atgtgttcgg gtcccatgat
     1981 gccgcgatcg ccggtcaccc aatgtagttg gacgccggcg agtgcaccgg ccgcgccctg
     2041 ttcataccgt acgatatgcg ccttgctctc ggcgatgact tcctggcctg gttgggtctg
     2101 aatgcggatg gctaattggt tggccatcgt acctgaaggt acgaacaatg c
//
LOCUS       DASTQY010000126         2974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1174812, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000126 DASTQY010000000
VERSION     DASTQY010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174812"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..992)
                     /locus_tag="VFR82_04015"
     CDS             complement(120..992)
                     /locus_tag="VFR82_04015"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4500794.1"
                     /translation="MTSQGASRFVLVTGASTGIGAACAIGCAQQGMTVFAGVRDLQAG
                     EALRARGGAAIVPVHLDVTDTKSIHEAAEFVEQRVGPEGLSGLVNNAGIAIASPLELL
                     PLAELRRQFEVNVIGQIAVTQALLPLIRRARGRIVNMGSIAGRGTIPMMGPYSASKYA
                     LEALTDALRLELYPWGIEVSIIEPGAIATPIWNKSLSTALTLETVIPPEGKRLYGESA
                     QRLREKVDESARRAIPPDAVVQAVLHALTARRPRTRYLVGADAKLRALMVKWLPDRLQ
                     DWILKKVLKLPPSV"
     gene            1147..2697
                     /locus_tag="VFR82_04020"
     CDS             1147..2697
                     /locus_tag="VFR82_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEU4500795.1"
                     /translation="MSKIAVIDVGTNSIHMVLAEVLPDFSYKILDRFKDIARLGDGAF
                     ASQRLSEEAMARGLSVIGRLATLAKNKGYDRIIAVATSAVREAKNGGDFIDLVKEQTG
                     LTVRVISGTEEARLIFLGIKNSVPMTEQAALAVDVGGGSVELMAGNRDQLLHAKSLKL
                     GAIRLADQFLKRTPPSDRMLRELEDLVTGQLAGTLESFKTKRFETLIATSGMAANLAE
                     VIHQRRTNRPLPQLNLATVSLKEVKEMEQVLRQSTIKERLAIPGLDPKRVDTLFPASL
                     VLRRLMELVGRDELILCDKAIREGVIFDFIVRHRERIKAEADIPDLRRRNVLALARRC
                     QVPEVHSLHVASLALRLFDQTKRLHNLGEIEREWLEFAAILHDIGYLINERQHHKHTY
                     YLITHSELGGLSAEEIVIVANIARYHRRALPHSKHDPFDALTPKLQRTVRIMSALLRI
                     ADGLDRTHFSVVRTLDVKIGATIKIIAYVTGDAELEAWAAKGRSDLFERIFRRRVQFT
                     LKPQEDAA"
     gene            2694..>2974
                     /locus_tag="VFR82_04025"
     CDS             2694..>2974
                     /locus_tag="VFR82_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500796.1"
                     /translation="MSEQPPNTPTPPHPYPGKLIIVEGIDGSGKSTQLLLLHKWLESK
                     GHKVFFTEWNSSELVKDTTKRGKKNKSLTPTTFSLLHATDFASRLYHEIL"
BASE COUNT          666 a          890 c          811 g          607 t
ORIGIN      
        1 ttgcaccggg ccttgttctc tcatctctca aaagctcaac gtacaacccc gtgttaatga
       61 gcttgtctaa cagcttgttg gggtaagacc cagctccgct agagttctga aacccaaatt
      121 tagaccgacg gcggcaattt gagcaccttc ttcaagatcc aatcctgcaa ccgatcaggc
      181 agccacttca ccatgagcgc cctaagcttc gcatcggcac caaccagata gcgggttcgc
      241 ggtcgccttg cggttaaggc gtgcaggacc gcctgaacca cggcatcagg tggaatcgcg
      301 cgccgcgcac tctcatccac tttctctctc aagcgttgag cactctctcc atacagacgc
      361 ttgccttcag gcggaataac cgtctcgaga gtgagcgcag tgctcagcga cttgttccaa
      421 atcggggtgg cgatggctcc tggttcgatg atcgatacct cgattcccca agggtacagt
      481 tccagccgca gcgcatcggt gagggcctcc aacgcatact tcgaggctga atacggtccc
      541 atcatcggta tggtgccgcg ccctgcgatt gagcccatgt tgacgatgcg tccacgtgcc
      601 cgccggatca agggaagcag cgcttgggtg accgcaattt gtccgatgac attgacttcg
      661 aattgacggc gcaattctgc taacggaagg agttctaacg gactcgcgat cgcaataccg
      721 gcgttgttca ccagtcccga cagtccctcg gggcccacgc gctgttcgac gaactcggcg
      781 gcctcatgaa tggatttcgt gtctgtcaca tcgaggtgaa ccgggacaat cgccgcgcct
      841 cccctggcgc gaagcgcttc acccgcctgc aaatcacgta ctccggcaaa tacggtcatg
      901 ccctgctgcg cacacccgat agcgcaggcc gcgccaatgc cggtggacgc gcccgtgacc
      961 aagacgaatc gcgaagcacc ctgactagtc attccagatt tccttcgact ctcgcggatt
     1021 gtaacagaaa gttaacaatc gcgactttta ttcgagatag atttcttcac taagccctct
     1081 ggattcatct acaatggcgt gacctattca gtatttcttg gatcctaaaa tcgtccgctt
     1141 tctgacatgt caaaaattgc cgttatcgat gtcggtacca actcgatcca catggtcctc
     1201 gccgaggtcc tgcccgattt cagttacaag attctcgacc ggttcaaaga tatcgcgaga
     1261 ctcggggacg gcgctttcgc cagtcaacgt ctgtcagagg aggcgatggc aaggggcctg
     1321 agcgtcatcg gccggctcgc gacgctggca aaaaacaagg ggtacgatcg gatcatagcc
     1381 gtggccacca gcgccgttcg agaagcgaag aacggcggcg acttcatcga tctcgtgaag
     1441 gagcagaccg gtctcaccgt ccgcgtcatc agcggaacgg aagaggcgcg tttgatcttc
     1501 ctgggcatca agaatagcgt gccgatgacc gagcaagcgg cgctggcggt ggatgtcggc
     1561 ggtggttcgg tggaactcat ggcgggcaat cgggatcaat tactgcacgc caagagcctc
     1621 aagctcggtg ccatcaggct cgccgatcaa tttctgaaac gcacgccgcc gtccgacaga
     1681 atgctgcgtg aacttgaaga cctggtgacc ggacaattgg ccgggacgct cgagagcttc
     1741 aagaccaaac gcttcgagac tctcattgcg acgtcaggaa tggcggccaa cctcgccgaa
     1801 gtgattcatc agcggcgaac gaaccgtcct ctcccgcagc tcaatctggc caccgtgtcg
     1861 ctgaaggaag tgaaggagat ggagcaggtg ctccgtcagt cgacgatcaa ggaacgcctg
     1921 gctattcccg gcctggatcc caaacgcgtg gatacactgt ttccggcctc gctcgtcctt
     1981 cgccgtctga tggaattggt gggacgcgac gagctgatcc tctgcgacaa ggccatccgc
     2041 gagggcgtga ttttcgattt catcgtccgg catcgcgagc ggatcaaagc cgaagccgac
     2101 ataccggatc tgcgacgccg caacgtgctg gctctggcga ggcgatgcca ggttccggaa
     2161 gtccatagcc tccacgtcgc gagtctggcg ttgcggctct tcgaccagac caaacggctg
     2221 cataatctag gcgagatcga gcgcgagtgg ctcgaatttg ccgccattct ccatgacatc
     2281 ggctacctga tcaatgagcg gcagcaccac aagcatacgt actacctgat cacccatagt
     2341 gaactgggag ggctctcggc cgaagaaatc gtcatcgtgg ccaatatcgc gcggtatcac
     2401 cggcgtgctc ttccgcacag caaacatgac cccttcgatg ctctcactcc taagctacag
     2461 cgaacagtcc gtatcatgtc cgcgttgctt cggattgctg acggcctgga ccggacccac
     2521 ttctcagtcg tcaggaccct ggacgtcaaa ataggcgcaa ctattaagat tattgcctat
     2581 gtgacgggcg atgccgaatt ggaagcctgg gcggcgaaag gacggtcaga tctatttgaa
     2641 cggatcttcc gccggcgggt acaattcacg ttaaaacctc aagaggatgc agcatgagtg
     2701 aacagccgcc caataccccc acgccgcccc atccgtatcc cggcaagttg atcattgtcg
     2761 agggcatcga tggctcgggc aagagcacgc agctgctgct cctgcacaag tggctggaat
     2821 cgaaaggcca caaagtcttc ttcacggaat ggaactcgtc tgaattggtc aaggacacga
     2881 ccaagcgcgg caagaaaaac aaaagcctga ctcccacgac gttcagcttg ctccatgcca
     2941 ccgacttcgc cagcaggctg taccatgaga ttct
//
LOCUS       DASTQY010000127         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1059585, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000127 DASTQY010000000
VERSION     DASTQY010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059585"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            802..1428
                     /locus_tag="VFR82_04030"
     CDS             802..1428
                     /locus_tag="VFR82_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HEU4500797.1"
                     /translation="MLLTLTGLGCQTAYYGTMEKLGYHKRDLLVRDVTKARDAQQEAK
                     EQFRSALDRFTKTLDIQGGELQEKYEALNSEYERSEAKANAVRNRIASVEDVSEALFR
                     EWNAELKEYSSSALRKKSQEQLTKTRTHYAQLIKVMKRAEAKMAPVLAKFKDQVLFLK
                     HNLNAQAIASLKSELASVEGNINSLIKDLNTSIQEADSFIAAMPKDND"
     gene            complement(1442..2545)
                     /locus_tag="VFR82_04035"
     CDS             complement(1442..2545)
                     /locus_tag="VFR82_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4500798.1"
                     /translation="MTSKFVRALSLMAMLLGPECFSTWTDVSSGAEPAVGVTEAELPS
                     YISVEGVQGRFTIAGSNTMYPLMTRLSGEFRRYYPDVHIAIEGQGSKSAVAEDDRRPF
                     WEMVQNKSTYRRGDGSDDGHQVSMQVQVMASSRRLSPKEVETFESRYGYRPMEIPIAL
                     EAVAVYVHKDNPIQGLTLDQVHAIFSNKSRRDVKEITTWGQLGFQDERREAGIHLYGR
                     DQRSGTRAYFKERVLLDEEFRTTLQEEPGSANLILAVGRDPLGIGYSGISFQTSFVRV
                     LPLAAKSGLPFVSPSAESAIDGSYPLTRTLYLYINKKPSERLSAALSEFLRFVNSRQG
                     QEIVVKAGVYALPAADIHQTQKKLIDAVAVLRK"
     gene            complement(2559..>2759)
                     /locus_tag="VFR82_04040"
     CDS             complement(2559..>2759)
                     /locus_tag="VFR82_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500799.1"
                     /translation="CDRAKEACPIWPGVIRMLHWSFEDPASAVGSDSEREAVFRNIRD
                     QIAFQIRSFLSKPFMPTGPFRN"
BASE COUNT          694 a          710 c          722 g          633 t
ORIGIN      
        1 gcgcaagagg cggtgaggga aggcagacat atgagagcag tccttctcta atgagtatgg
       61 tgcaacgccc ggacggctaa tgcgagcaat ctctatgcca ttcttgagcg gcgcgaattc
      121 caaagaaatg cggggtttta ctcgccaatc gtatcttgct gtggtcaaaa ttgttcggcg
      181 tgtttctgag aacatatggc gtttaaaaac ggtgaatatg gctttactgg ctggctgtca
      241 gccatgttgg taacgggcgt gatggcagct agtatatttc cgcaattcgt ttggtggatt
      301 gtgccgacaa ggccaaagcc atagagtgat ggggtgggaa gacgagttca gtacagaaac
      361 tgcatcatca agtccaaccc tgagctggtc gctgcttcag gtgaatggag gctacggatt
      421 gcgatctact cgcagacgaa tggcgttttg aacatgcaat ccttctcagg gcctacggtt
      481 tacaatagcg aggaagaagc tgatgtccat ggtatcgcgt atggccaacg catcattgat
      541 gagaaggtgc ctggattgaa ggcgggttag ctaagctgcc acatacatcg tcaggtgcca
      601 cctagtcttg ggaaacactt caatagcggc agactttgac gtgaggcgag cggctaagag
      661 gcccaaagcc gacctattgg ttcttccgca gcctatagag taccctacat aaccatggac
      721 ttttcacagg atgacacggt gcatcttaaa cattcccttt tgaacgttcg taaactgtct
      781 ttccccctga catatgggat catgctgtta actcttaccg gcttgggttg ccagactgcc
      841 tattatggaa cgatggaaaa gcttggctat cacaagcgcg acctattggt ccgcgatgtc
      901 acaaaagccc gtgatgctca gcaggaggcc aaagaacaat ttagatcggc tctcgaccgc
      961 ttcaccaaaa ctttggacat ccagggcggg gagttacaag agaagtacga ggccctaaac
     1021 tccgaatacg agcggagcga agccaaagcg aacgcggttc gaaatcggat tgcgtcagtg
     1081 gaagacgtgt ctgaagcgct gtttcgtgaa tggaatgccg aacttaagga gtactctagc
     1141 tccgctttgc gcaaaaagag ccaagagcag ctgacaaaga ctcgcacgca ctatgcgcaa
     1201 ttgatcaaag tcatgaaacg ggccgaagcg aagatggcgc ccgtgttggc gaagttcaaa
     1261 gaccaggttc tttttctaaa gcacaacctg aacgcccaag ccatcgcgtc attaaaaagt
     1321 gaattggcct cggtggaagg gaacatcaat tcactcatta aggatctcaa cacctccatt
     1381 caagaggccg attcctttat tgccgccatg ccgaaagaca atgattaggc aaagttccca
     1441 cttattttct gaggacggct actgcgtcta ttaatttctt ctgagtctga tggatatcag
     1501 ccgccggtaa tgcgtacaca cctgccttca ccacaatctc ttgcccctgt cggctgttca
     1561 caaatctcaa aaactcactt agagcggcac tcagtctctc agacggcttc ttgttgatat
     1621 agagatataa tgtccgggtc agcggatagc tgccatcaat tgctgactcg gcacttgggc
     1681 tgacgaatgg aagacccgat ttagccgcaa gcggaagcac gcggacaaac gacgtttgaa
     1741 agctaattcc gctgtaaccg ataccgagag gatcccgccc tacggccagg ataaggttgg
     1801 ccgaccctgg ttcttcttga agcgtagtcc ggaactcttc atctagtaga acacgctcct
     1861 tgaaatatgc tcgcgtaccc gatcgctggt cacgaccgta taagtgtatg ccagcttccc
     1921 gacgctcatc ttggaagcca agctggcccc acgtcgtgat ctcctttaca tcacgtcgtg
     1981 atttgttcga gaagatggcg tgcacttgat caagcgtgag accttggatg ggattatcct
     2041 tatgcacata tacggcaacg gcttccaagg caatgggaat ttccatggga cgatagccgt
     2101 agcgagactc gaacgtctca acctccttag gagataggcg gcgggaggag gccatgacct
     2161 gcacttgcat ggatacctgg tgcccgtcat cgctcccatc accgcgtcga tacgtcgact
     2221 tattctgaac catttcccag aagggtcgtc ggtcgtcctc tgccacggcc gatttggaac
     2281 cctgcccctc tatcgcgatg tgaacgtcgg gataataccg tcgaaattca ccactcaagc
     2341 gtgtcatcag aggatacatg gtgttggatc ccgcaatcgt aaagcgtcct tggactcctt
     2401 cgacggaaat gtaggacggt agctcagcct ccgtcacacc gacagccggc tctgctccgg
     2461 aactgacgtc tgtccacgtg gagaagcact ccgggcctag tagcatcgcc atcagggaaa
     2521 gggcacgaac aaacttggaa gtcatggccg atcctcctct agttgcggaa tgggcccgtc
     2581 ggcataaacg gctttgaaag gaaggaacgg atttgaaagg caatttgatc gcgaatgttc
     2641 ctaaacaccg cttcgcgctc gctgtcagat ccaacggccg aggccggatc ttcaaaactc
     2701 caatgcaaca ttcggatgac ccccggccaa atgggacatg cctctttggc ccggtcgca
//
LOCUS       DASTQY010000128         6672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_51509, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000128 DASTQY010000000
VERSION     DASTQY010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51509"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFR82_04045"
     CDS             complement(<1..215)
                     /locus_tag="VFR82_04045"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEU4500800.1"
                     /translation="MQTYVSLVKFTQHGLQSMKDKGIERAEAVKHNAQSLGGKLIQAY
                     YCLGEYDVIAIWEFPDNKTAMKAAVLNA"
     gene            504..746
                     /locus_tag="VFR82_04050"
     CDS             504..746
                     /locus_tag="VFR82_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500801.1"
                     /translation="MDIQRAVKAFIAEGKDLYHRLRSSGEGLSDLDLVALREQLYILD
                     TEAGHLQELKDSQSNGAEFIFSNRPPVVQPFSRKAA"
     gene            979..1128
                     /locus_tag="VFR82_04055"
     CDS             979..1128
                     /locus_tag="VFR82_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500802.1"
                     /translation="MKAGYRLILVDHDGVMVSEFQLTERDLADPPAFVAAIKQSIEQV
                     EDEEP"
     gene            1115..2248
                     /gene="ydiK"
                     /locus_tag="VFR82_04060"
     CDS             1115..2248
                     /gene="ydiK"
                     /locus_tag="VFR82_04060"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HEU4500803.1"
                     /translation="MKNLEIQPPETDLTESDRPMSAPTTPHDLARSTLSVLVMVGLIA
                     ASFWILRPFIPAMLWATMIVVAGWPLMLQLESLLWGRRGLAVTVMTLALLLVFVVPFS
                     LAVTALVTHLDEIVDWAQSLKSLTLPAPPSWVESIPLAGTRIASSWRDFASSGPDELA
                     RSAAPYAKVTGSWLLAQLGSLGAMTGQFLLTVLIAAVLFARGDSAVADLRRFGRRLAG
                     ARGEQAVNLAGQAIRGVALGIVLVAMGQAVLGGIGLFVAGVPFAAVLTALMFLFGIVQ
                     VGVVPVLAVAVTWLYWQGSHGWATGLLAWTIFVGTIDNVIRPILIQKGARLPLVLIIA
                     GVIGGLVAFGVVGIFVGPVVLGVGYMLFEAWIQEEPLDSEEGV"
     gene            2285..3328
                     /locus_tag="VFR82_04065"
     CDS             2285..3328
                     /locus_tag="VFR82_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEU4500804.1"
                     /translation="MRERRLPTITLRQSKPRSSLAPCLVSLFLVAAPVFGGTPEREQD
                     RGAWRTAAPAAMKRTEVAAATVGGKIYLVGGFEEPSISNVVNLSITPVVEEYDASTDR
                     WRSKAPMPVGLHHVGIGVVGGRLYVIGGYKQSGLSVWGPVATLYSYDPAADAWTELPA
                     MPTARGALSVTAHEGKLYAIGGFDGKGNSAAVEVYDPVRNAWSTRASLPTPRDHLATA
                     TLAGKVYAIGGRVKGDYHRNLSVTEVYDPGLDKWTKAPDLPTARSGITAAEMGGRVYV
                     FGGEGANGTFHENEAYDPTRDAWQAMAPMPTARHGLGSSVIDGHIHVISGGPTPGGSF
                     SNLNEVFSPPGSR"
     gene            complement(3344..3952)
                     /locus_tag="VFR82_04070"
     CDS             complement(3344..3952)
                     /locus_tag="VFR82_04070"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEU4500805.1"
                     /translation="MYARRSIDPPGRLYWEVMMKLLSIQVGRPRKVKWRRRTVTTGIY
                     KAPVEGRIMLRPHNLEGDEQADLSVHGGWDKAVYVYPSEHYAFWRTELPGMQLPYGMF
                     GENFTTEGADESTVYIGDRFSIGSAVVEVTQPRMPCYKLGVRFERPDMPQRFHASGRC
                     GFYLAVVTEGNVGTGDVWELLDRRDNTMSVLDRYRSFFEEAG"
     gene            complement(3955..4854)
                     /locus_tag="VFR82_04075"
     CDS             complement(3955..4854)
                     /locus_tag="VFR82_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4500806.1"
                     /translation="MSAHTPSIRYCKQEIDGINIFYRAAGSPASPAILLMHGFPSSSH
                     MFRGLIPLLAQHFHVIAPDLPGFGFSDAPSRSHFAYTFDHLTDVMERFTEAIGLARYA
                     VYIFDYGAPVGLRLALRHPERITAIVSQNGNAYEEGLSAGWNPIQRYWAEPTQENRNA
                     LKTFLSPDATKWQYTQGTDESRIAPESYTLDSALLARVGNEDIQLDLFLDYGNNVTLY
                     PRFQQYFREHQPPLLAVWGKGDPLFLPAGAHAFTRDNPNAQVAFFDTGHFALETHGTE
                     IGAKVVEFLRALTPVEVGVSASE"
     gene            4989..5609
                     /locus_tag="VFR82_04080"
     CDS             4989..5609
                     /locus_tag="VFR82_04080"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEU4500807.1"
                     /translation="MDLKVRHEIPFLFVGNHPCLDFINTDLVINGQPTDLLSSFQDLL
                     VWLVQASLVTDEEAKRIERVGKRQGNDTLKEAKAFRTAMRDMVRRLADGEPVPEAALE
                     TINHLLRLRMGYSQISRRSGGTFELNYHSETEDANRLLGLLGKAASDLLATCNLSLVK
                     KCQNPACVLFFYDTTKNHARHWCSMSLCGNRSKVAAHYLRHRKDRP"
     gene            5606..6229
                     /locus_tag="VFR82_04085"
     CDS             5606..6229
                     /locus_tag="VFR82_04085"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEU4500808.1"
                     /translation="MTPKERTVVGVDVGGMKKGFHAVALRDNQIVAKLTTCSAVDVAT
                     LCRKQGATAVGIDAPCQWSLAGRARSCERELAGLGISVFCTPSRAVGQIHPFYSWMVN
                     GAELFRLLVHDYRLYDGRTAPLDPLCFETFPQAIACSLAGTKLSAKNKRADRRRLLVQ
                     AGISSDALTTIDDIDAALCALSAQHVLAGRFKAYGDAAEGFILLPAC"
BASE COUNT         1328 a         1888 c         2039 g         1417 t
ORIGIN      
        1 gcattgagga ccgcggcttt catggccgtc ttgttgtccg gaaattccca gatggcgatg
       61 acgtcgtatt cgccgaggca gtagtaagcc tggatgagct ttccgcccag ggattgggca
      121 ttgtgcttga cggcttcggc tcgctcgatc cccttgtcct tcatcgattg gagaccgtgt
      181 tgcgtgaact tcacgaggct aacgtaggtc tgcatagtgg gtcctcctaa gtgagcggtg
      241 cagtgctgtg ttcgaccaaa ttgtagggga atggagggga tggatgatag accccgctgc
      301 tcgagcgaga ggtggaccgc atggcctcca tctcccggag cgggaagaaa gaggagggct
      361 caggaccatt catatgatgt gcgtgcctgg gagacatcac cctacacaga caaggggttt
      421 tactaggctg accgctgcta aaagcagcac atagtaggac gaccaggcgt tgcgcatcac
      481 acagaaacat ggaggcagga accatggata ttcaacgcgc cgtcaaggca ttcatcgcag
      541 aagggaagga tttgtaccac cggctccgct ccagtggtga gggactgtcc gatctggatc
      601 tggtcgcgct ccgagaacag ttatacattc ttgatacgga ggccggtcat ttgcaggagc
      661 tcaaagattc ccaatcgaac ggagccgagt tcatcttcag caacaggcca ccagttgtcc
      721 agccgttcag ccgcaaagcg gcctagttga acatagccgt tccgaatcgg tcataagatc
      781 cgacgtcgcc tcacccccgc ggttatgtcc gccggggagg gcctattctg atttcgcggt
      841 ccgtcggtat gcctagcttt gagaatcgcc tgccgagcgc cagtggttga aacggcacct
      901 gaagtacccg tttgacaatg agtggatatt ggggggagga tcacgttcct caggccagcc
      961 ctaccgagag gagtgaccgt gaaagctggt tatcgtttga tccttgtcga tcacgacggc
     1021 gtgatggtgt cggaattcca gctgacggag cgcgacctgg ccgatccccc ggcgtttgtg
     1081 gccgcgatta aacagtcgat cgagcaggtt gaagatgaag aaccttgaaa tacagcctcc
     1141 tgaaacggat ctcactgaga gcgaccggcc gatgagtgcg ccgaccacac cccatgatct
     1201 ggcgcgatcg actctatccg ttctggttat ggtcggcctc atcgccgcct cgttttggat
     1261 tctgcggcca tttattcctg cgatgctctg ggcgacgatg atcgtcgtcg cgggttggcc
     1321 attaatgttg cagctcgaat cgttgctctg gggacggcgc ggcttggccg tcacggtcat
     1381 gacgttggcg ctgttgctcg tattcgtcgt tcccttttcg ctggcggtga cagcgctcgt
     1441 gacccatctc gacgagatcg ttgactgggc gcagtcgctc aaatccctca ctctgcctgc
     1501 gccaccatcc tgggtcgaaa gcatccctct cgccggtacg aggatcgcct cttcatggcg
     1561 cgatttcgcc tcttccggtc ctgatgagct cgcccgatcc gcggcgccgt atgccaaggt
     1621 gaccggaagc tggctgctgg ctcaactcgg cagtctgggt gccatgaccg ggcagtttct
     1681 gctcacagtc ctgattgcgg ccgtgctctt cgctcgaggg gacagcgccg tggcggatct
     1741 gcgccggttc ggacgacgcc tcgcaggagc acgcggcgaa caggcggtga atctggccgg
     1801 gcaggcgatt cgcggcgtcg cgctcggcat tgtcctcgtg gccatgggcc aagcggtgtt
     1861 ggggggaatc ggtctgtttg tggcgggtgt gccgttcgcc gcggtgttga cggcgttgat
     1921 gtttctgttc ggcatcgtgc aggttggcgt cgtgccggtg ctcgctgtcg ccgtcacctg
     1981 gctctattgg cagggaagcc atggctgggc cactggactc ctggcctgga ctatctttgt
     2041 cgggacaatc gacaacgtga tacggccgat tctcattcaa aagggcgcgc ggttaccgct
     2101 ggtgctcatc atcgccggcg tcattggcgg tctggtcgca ttcggcgtgg tgggaatttt
     2161 cgtgggaccg gtcgtgctgg gagtgggtta tatgttgttt gaagcctgga tacaggaaga
     2221 accgctcgat tctgaggaag gcgtgtagat gtggctcatc ggcaggcgat tgatgaggca
     2281 ggccatgcga gagagacggc taccgacaat cacactccgt cagtccaaac ctcggtcatc
     2341 gttggcaccc tgccttgtat ccctgtttct cgtcgcagca ccggtttttg gcggaacgcc
     2401 ggagcgggaa caggatcgtg gtgcctggcg cactgcagcg ccggcggcca tgaagcggac
     2461 ggaagtggct gccgcaacgg tcggaggcaa gatctatctc gtgggcgggt tcgaagagcc
     2521 gagcatcagc aatgtggtga atctctcgat tacacccgtt gtcgaagagt acgacgcttc
     2581 taccgaccga tggagatcga aggcgccgat gccggtcggt ctgcatcatg tgggtatcgg
     2641 cgtggtcggc ggacgcctgt acgtgatcgg cggctataaa cagtccggcc tcagcgtctg
     2701 gggaccagtc gccaccctgt attcctacga tccggccgcg gacgcctgga cggagctccc
     2761 cgcgatgccg accgcccggg gggccttgtc ggtcacggcg catgaaggca aactgtacgc
     2821 gatcggtggc tttgatggaa agggtaacag cgcagccgtc gaagtgtatg atcccgtgcg
     2881 aaacgcttgg agcacgcggg cgtcgctgcc gactccgcgt gatcatctgg cgaccgcgac
     2941 gttggccggg aaggtctacg ccatcggcgg gcgagtgaaa ggcgactacc atcgcaatct
     3001 ttccgtcacg gaagtgtacg atcccggctt ggacaagtgg actaaggcgc cggaccttcc
     3061 aacggccagg agcgggatca ctgccgctga aatgggaggg cgagtctatg tgttcggcgg
     3121 cgaaggcgcg aacggcacct tccatgaaaa tgaagcctac gatccgacac gcgatgcctg
     3181 gcaggctatg gcgccaatgc caacagcacg tcacggcctc gggtcgagcg tcatcgatgg
     3241 ccacatccat gtgatcagcg gcgggccgac gcctggcggc tcgttcagta acctcaacga
     3301 ggtcttctca ccaccgggga gccggtgagt ccgttggcac cgctcacccc gcttcctcaa
     3361 aaaaactacg atacctgtcg agcacggaca tcgtgttgtc ccttcggtcg agaagttccc
     3421 aaacatctcc ggtcccgaca ttaccttccg tcaccacggc cagataaaag ccgcaccggc
     3481 cgctcgcatg aaaccgttgt ggcatgtcgg ggcgttcgaa ccgcacgccg agtttgtagc
     3541 agggcatgcg gggctgcgtg acttcgacca ccgcggagcc gatactgaat cgatcgccga
     3601 tgtagacggt cgattcgtcc gctccttccg tcgtgaaatt ctcaccaaac atgccgtaag
     3661 gcaactgcat gccgggaagc tcagtccgcc aaaacgcata atgctcggat ggatagacat
     3721 agaccgcctt gtcccatccc ccgtgcacac tcagatcggc ttgctcgtcg ccctctagat
     3781 tgtgcgggcg cagcatgatg cgcccctcta ccggggcctt gtagatgccg gtcgtcacag
     3841 tcctgcgtcg ccactttacc ttgcgcggac gacccacctg aatcgaaagc agcttcatca
     3901 tgacctccca atagagccgg cccggcggat caatgctgcg cctcgcgtac atcttcactc
     3961 actggcactg actccaactt ctaccggcgt cagcgcgcgc agaaactcca cgaccttcgc
     4021 accgatttcc gtgccgtgcg tttcgagcgc aaaatggccg gtgtcgaaga aggcgacttg
     4081 tgcattcgga ttatcccggg tgaacgcgtg cgcccctgcc gggagaaaca atggatctcc
     4141 tttgccccag acggcaagca gtggcggttg atgctcacgg aaatactgct gaaagcgcgg
     4201 atagagcgtc acgttgttgc cataatcgag aaacagatcg agttgaatgt cctcgttacc
     4261 gacgcgtgcc aatagggcgc tgtcgagcgt ataggattcg ggtgcgatgc gcgattcatc
     4321 ggtgccctgc gtgtattgcc acttggtggc gtcgggcgag agaaaggttt tcaacgcgtt
     4381 tcggttttct tgagtgggct ccgcccaata ccgttgaatc ggattccagc ctgcgctgag
     4441 accttcctcg tacgcgttcc cgttttggga cacgatggcc gtgatccgtt cgggatgacg
     4501 gagcgccagt cgaagaccaa caggcgcacc gtagtcgaag atatagaccg catagcgagc
     4561 gaggccgatg gcttccgtaa agcgttccat cacatcggtc aaatgatcga aggtataggc
     4621 aaagtgtgac cggctgggcg catccgaaaa gccgaatccg ggcaggtcgg gagcgatgac
     4681 gtgaaagtgc tgggcaagca gcggaatcag cccgcggaac atgtgcgatg acgagggaaa
     4741 gccatgcatc aggaggatgg cgggagatgc tggcgagccg gctgcgcgat aaaaaatgtt
     4801 gattccgtct atctcttgct tacagtagcg aattgacggg gtgtgagcag acatacgaac
     4861 tcctttggtg taggcggccg gaattgccgg cggcaggtga tgaattgtgc ggaatggccg
     4921 tggaacatac gctaactatc tatacaatat attgacggtt atatcagtgt tgggctaacc
     4981 tgtcaagtat ggatttgaag gttcgacatg aaatcccctt tctcttcgtc ggcaatcatc
     5041 cgtgtttaga tttcatcaat acggacttgg tgataaacgg acaaccgacc gacttgttat
     5101 cgtcgtttca ggacttgctc gtctggctcg ttcaggcgag tctcgtaact gacgaggaag
     5161 ccaaaaggat cgagcgggta gggaagcgcc aggggaatga caccttgaaa gaggcgaagg
     5221 cgtttcgtac cgccatgcgt gacatggtcc gacgtctcgc agacggcgag cccgttccgg
     5281 aagcggcgct cgagaccatc aaccacctgc tgcgccttcg gatggggtat tctcagatca
     5341 gtcggcgaag cggcggcaca ttcgagctga actaccactc ggagaccgag gacgccaatc
     5401 gactgctcgg gttgctgggg aaagcggcga gcgatctcct ggccacgtgc aatctgtcat
     5461 tagtcaagaa gtgtcaaaac ccggcgtgcg tacttttttt ctacgacacg accaaaaacc
     5521 atgcacggca ctggtgcagc atgagtctct gcggaaatcg cagtaaggtc gccgcccact
     5581 atcttcgaca ccggaaggac aggccatgac gccgaaagag cgcaccgtcg tcggcgtgga
     5641 tgtcggcggt atgaagaagg gcttccatgc cgtcgccctg cgggacaatc agattgtggc
     5701 caagctgacg acctgcagcg ccgtggatgt ggcgaccttg tgtcgcaagc aaggcgcgac
     5761 agccgtcggc atcgatgcgc catgccagtg gagtcttgcc ggacgggcca gatcctgcga
     5821 acgagagcta gccggcctcg gcatctctgt cttctgtacg ccgagccggg cggtcgggca
     5881 aattcatcct ttttacagtt ggatggtcaa cggcgccgaa ctcttccgac tgctcgtgca
     5941 cgactaccgg ctttacgatg gtcgaactgc gccgctcgat ccgctgtgct tcgaaacatt
     6001 cccgcaagcc atcgcctgct cattggccgg tacgaaactc tcggccaaga acaaacgggc
     6061 ggatcgtcgt cggctcttag tgcaagcagg catatcgagc gacgctctca cgaccatcga
     6121 cgatattgat gctgcgctat gcgccttgag cgctcagcat gttctcgctg gccgctttaa
     6181 ggcctatggt gacgccgccg aaggcttcat cctcttgcct gcctgctagt tcctgtcaaa
     6241 aagctattca acgattccca agcgagcgct gcgcttgaga gagcatgtat tgccagctcg
     6301 tctgtactcg tgtgtcaccg tccgtaagga tgtatggcct tacggcgact tgcgaccggt
     6361 cgtatgacgc tgccgtgcgc tcgcgtcagc gatggcgagc atccacatgc tcttcaacga
     6421 gtgctccttc tatcttgaag acggggctga ttggtctctc actgcgcgct tggacgagcg
     6481 ccgaccttac tgaaattacg attaagcccg cgcgcgttcg gcgagcacga gagaccaaca
     6541 ggcccgtcga tttggtcttg acttcgttct gcacttcgat tacaaaagcc aacgttcatt
     6601 ggcgtgggca tgcaggaaca ggaagctggc tgcacccgta acgcactccg cctcgttcac
     6661 atcctctcat ag
//
LOCUS       DASTQY010000129         7597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_51374, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000129 DASTQY010000000
VERSION     DASTQY010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51374"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..670)
                     /gene="bcp"
                     /locus_tag="VFR82_04090"
     CDS             complement(203..670)
                     /gene="bcp"
                     /locus_tag="VFR82_04090"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEU4500809.1"
                     /translation="MAKDIAVGDKAPDWSLPDQTGKPVTLKSLKGKQIVLYFYPKDDT
                     PGCTKESCDFRDVESQIIRAGGVILGVSLDGKESHQKFVKKFGLPFPLLSDEDAAVSK
                     AYGVYKEKNMYGKKSWGIERSTFVIDPDGKLKAVFRKVKVDGHVDEVLTALRS"
     gene            complement(684..1124)
                     /locus_tag="VFR82_04095"
     CDS             complement(684..1124)
                     /locus_tag="VFR82_04095"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4500810.1"
                     /translation="MKFGWPVIVAVLLIVCLGACTKGKARPLVPLALEPHAKAPAVAL
                     TEQGTQAYQTRKFDEAKRYFSQAVTEAPQSGPAHYNYALALNALGDTEEARQHFIDAA
                     NLAPGDKTIWDSPALHQYGNPQTEKETKEHRTTTSRPTFGGPPR"
     gene            complement(1150..1422)
                     /locus_tag="VFR82_04100"
     CDS             complement(1150..1422)
                     /locus_tag="VFR82_04100"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HEU4500811.1"
                     /translation="MLSEDKVSHLSHVILLAVKKSPLVEVRSDDARILKDIKRVLASE
                     LAQEQDIDRKVRTKLGSYSRGIVEGSSEWDVLYRKTFEEESRKHTK"
     gene            complement(1482..1763)
                     /locus_tag="VFR82_04105"
     CDS             complement(1482..1763)
                     /locus_tag="VFR82_04105"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HEU4500812.1"
                     /translation="MRLSKERVRHMAETLAGRLVQEGHVALTGTQKAFIESLDQVVTD
                     ELSIEERLNGEVRQLLKAYEQQIETGQVDYQKMFTMIKQKLVRERGIIL"
     gene            complement(1767..2201)
                     /locus_tag="VFR82_04110"
     CDS             complement(1767..2201)
                     /locus_tag="VFR82_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HEU4500813.1"
                     /translation="MGAGFQSQRFTHGEHRSLTDLGNKIRIIVGGVQVEAELKGTKTA
                     REVYAALPVEAPVNTWGEEFYFKLDGVRDYRETATTQVKVGDVAFWGAGQVLAIFFGR
                     TPMSMGPDPVPADRVNVIGRILGDPTVLRGVMAAPSIRVERV"
     gene            2255..3061
                     /locus_tag="VFR82_04115"
     CDS             2255..3061
                     /locus_tag="VFR82_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEU4500814.1"
                     /translation="MLTSTFVLLKGIGAATERRLWQDGVADWSTFLNRSLITGISPTR
                     KGWYDEVLATAQSRLAGGSVDFFRTCLKSREHWRLFHTFRHRALYLDIETTGQSSGQA
                     VITVVGLYRNGRMSSLVRGENLTEDRLAEELERTDLLVTFFGSAFDIPFLQLAFPRLN
                     LKKPHFDLCFAARRLGLTGGLKYIEREMQIDREPEIQGLDGWDAVRLWYQWRGGDEGA
                     LDLLLRYNAADTRNLEPLAELLYEQMVSRFGPPSVGPSPRACETTGSMIL"
     gene            3058..3867
                     /locus_tag="VFR82_04120"
     CDS             3058..3867
                     /locus_tag="VFR82_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEU4500815.1"
                     /translation="MKQSPRWLGVGRTETGHVRKTNQDVFAAINSCNFWIIADGMGGH
                     PAGDVAAHVAVDVATQTAKTLLSTREQPSQPPDKSLIETVIAANRAIHDKIREDSALK
                     GMGTTVVAMTIPPGPTPVAHVAHLGDSRAYRLHAGVLTQVTRDHTLVEVLLQRGLINE
                     ATARAYPDRHVLAKGLGMGLEMTPDLSSTPLAPDDTLLLCSDGLTKMLSDAEIGSILG
                     ATHANPRRACHELVEQALRAGGEDNVTVIVCAYTTPAKVGSLAIDKTPPAM"
     gene            3938..4801
                     /locus_tag="VFR82_04125"
     CDS             3938..4801
                     /locus_tag="VFR82_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU4500816.1"
                     /translation="MNRGRCVWVLSAIVMLASVSSGRAAEDQSAEATVRALVRANAEK
                     DMETMSRLMAHDADITSYTIGGRKYVGWVDLERDMEEEFRSAAIIEIPIRELKVWTRG
                     DLAWYAMEVDYIRTVTAGSQPQRALLPLRETGVLERRDGQWVLLSWHESFRNAGGAVA
                     LNEHASVTLATSNNHLAQTDLSGEWEILEVEDDKRYRATLDKAGNGPYTQHGGRFVTS
                     RFSDRLWQGTWQQPGNDREGGFEILLSEDGKEAKGVWWYTRVGTHKNIPPREHGGTYQ
                     WKRITPVPTAP"
     gene            4888..5880
                     /locus_tag="VFR82_04130"
     CDS             4888..5880
                     /locus_tag="VFR82_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500817.1"
                     /translation="MRVLLAVLLCLWSAPVMADGGHDHHAVPTLKNQTTTVVTIKDNA
                     VTLTFGPIDLPSGHEGDLAASMPKHVFQLPKDMYMVGFKSAVFTKDGTPLPRNYLHHI
                     LMLNNDKPSVSCPGEPLFFSGAGLEMTEAKFPDGYGVKLGKGQRLMSVVAFYHKAPPT
                     RNVMASFTMYMAPENTPVKELEVYQVGVNVVCYSKFAQRGPDQTDEGIEIKPGVQVHS
                     APVKFSMDGCVKFAYPHGHDELLLIALENKTRQQTLLRTVPDVRLDGTFLEFQPHQVY
                     KDPQGFSVSKEEDYEMVMVHHHPLQKQDPQHGMGNYLLYMTPGTCPTQKTASAN"
     gene            6172..>7597
                     /locus_tag="VFR82_04135"
     CDS             6172..>7597
                     /locus_tag="VFR82_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4500818.1"
                     /translation="MPQSEPITILVINEHAEEIKVVTISLRGFFHDVRIDAAYSTEEA
                     SRMASTTTSGWAVVLIDEGSLPSGRSTLLHDLKRQIPHASVILQSTRTDAAAALEALE
                     NGADFFLNKQSPAFLTELLFCAKEAFDKRDLRVSADRAQVRHSWLVDSLTEIVYELDA
                     DGRFLTLGRSLSVLLGYHPDELIGRPYYTLFPQDQQSLARFRFNERRTGVRASTGVEL
                     TLLGKPTAAGESQVTAAISARGVYDLSRRFVGTVGLIREVREQTKAQPLRHEIGPQQR
                     TDDLHALVRQMRDLAQRLQQPLSTLLQEAQQLSDRLREFGVIERLGALIEHAAASAQL
                     GEQLSRAAEEAPRTDTINRLLEDLLTSAFSDYAAGGLVSDLTSALPPYEGDREKTTRL
                     LRRLLVYAGCFLTAVGRAHGLAVKTSVDTPPAFTDAPTLFPLSQSRQVQVDITETEHT
                     VVAPAWPTRDSPLDAAELFQLAREL"
BASE COUNT         1641 a         2234 c         2095 g         1627 t
ORIGIN      
        1 tggtttcgat ggtggtctgt tggtggggtg ctgtaaggct atctttcaca agaactgtgg
       61 cggcgatctt ttccaccgcc ggtacaggac cggcgacggt caggaaaaga accagagaca
      121 ccggcgcggt tgtgcggata aaaggcgaga agggcatcga ccggcttcaa gccatgggat
      181 tacgaagaat cagttggaaa gactacgacc gtaacgccgt caagacttcg tcgacatgcc
      241 cgtcgacttt gaccttgcgg aaaacggcct tgagcttgcc gtcagggtcg atgacaaagg
      301 tactgcgctc gatgccccag gactttttcc cgtacatatt tttttctttg tacacgccgt
      361 aggccttcga gacggcggca tcttcatcgc tgagcaaggg aaacggcaag ccaaattttt
      421 tcacaaattt ttggtgtgac tccttgccgt cgagactgac accgagaatg acgccacctg
      481 ctcggataat ttgtgattcc acgtcccgaa aatcgcacga ctccttggtg cacccgggcg
      541 tatcgtcttt cggatagaaa tagagcacga tctgtttgcc cttcaaactc ttcaatgtca
      601 ccggcttgcc agtctggtca ggcagtgacc agtctggcgc tttgtctccg actgcgatgt
      661 ccttggccat atcataatcc tctctaccga ggagggccac cgaacgtcgg gcggctcgta
      721 gtcgtccgat gttccttggt ttccttttca gtttgcgggt tcccgtactg atggagcgcc
      781 ggcgaatccc aaattgtttt gtcgcccggc gccagattag ccgcatcaat aaaatgttgt
      841 ctcgcttctt cggtatcacc caacgcgttc agcgccagcg cgtaattgta atgtgccgga
      901 cccgattgcg gcgcctccgt gaccgcctgg gaaaaatacc gtttggcttc gtcgaacttc
      961 cgggtctgat aggcctgcgt tccctgctca gtcaaggcca ccgccggggc tttggcgtgg
     1021 ggttcgaggg ccaagggcac gagcggcctg gcttttccct ttgtgcaggc tcccaggcag
     1081 acgatcagca acaccgcgac gatgactggc catccgaatt tcatgacacc ctcctactca
     1141 tacgccttct catttcgtgt gtttccgcga ctcttcttcg aacgtcttgc gatagagcac
     1201 gtcccactca gaactgcctt cgacgatgcc gcgggagtac gagcccagct tcgtcctcac
     1261 ttttcggtcg atatcctgtt cctgtgccaa ttcgctggca aggacgcgtt tgatatcctt
     1321 caatatccgt gcatcgtcgc ttctcacctc aacgagcggg cttttcttga ccgcaagcag
     1381 gatcacgtgc gacaaatggc tgactttgtc ctcgctcagc atatggtcag tccgtcttga
     1441 ccggccgtct attgcctcga tcagcaagcg cctgatcgcg tttacaggat gatgccccgc
     1501 tcccgcacca acttctgctt gatcatggta aacatctttt gatagtccac ttgcccggtt
     1561 tcgatttgct gttcataggc tttgagcagc tgccggactt cgccgttcaa ccgttcttct
     1621 attgacaact catcggtcac cacctggtca agcgactcga tgaacgcctt ctgtgtaccg
     1681 gtaagggcaa catgaccttc ttggaccaac cgcccggcca gagtctcggc catatgccga
     1741 acccgctctt tggaaagccg catgcgttac acccgctcga cgcgaatcga cggcgccgcc
     1801 atcacacccc gcaacaccgt gggatctccc aggatgcgtc cgatcacgtt gacacggtcg
     1861 gcgggcacag ggtccggtcc catactcatc ggcgtccggc cgaagaaaat cgccaacact
     1921 tggcccgctc cccaaaacgc aacatccccg accttgacct gggtcgtcgc cgtttcccga
     1981 taatcccgga cgccgtccag cttaaaataa aactcttcgc cccaggtgtt cacgggtgcc
     2041 tccaccggca acgccgcata gacctcacga gccgtcttcg tgcctttcag ctccgcttca
     2101 acctgcaccc ccccgacaat aatgcggatt ttgttcccga gatcagtcaa cgaacgatgc
     2161 tctccgtgcg tgaagcgctg cgattgaaaa cccgctccca tcgcggactc tagcttgttt
     2221 aaccggtgaa atcaaggcta caatcccctt cgccatgctg acctcgacct tcgtactgct
     2281 caagggaatc ggtgcggcaa cggaacgccg cctgtggcag gatggcgtcg cagattggtc
     2341 gacctttctc aatcgttctc tcattaccgg catctcaccg acgagaaaag gctggtatga
     2401 cgaagtcctt gcgaccgccc aatcacggct tgccgggggc agtgtggact ttttcagaac
     2461 ctgtctcaag agccgggagc attggcgtct gtttcacacg ttccgtcatc gcgcactcta
     2521 tctcgacatt gagaccaccg ggcagtccag cggccaagcg gtaatcacag tggtgggctt
     2581 gtatcggaac ggacgcatga gcagtcttgt tcgcggggag aatttgaccg aggaccgcct
     2641 cgcggaggaa ctcgaacgca cggatttgct ggtgacgttt ttcggaagcg cgttcgacat
     2701 tccatttttg caactggcct tcccgcggct caatctgaag aaaccgcatt ttgatttgtg
     2761 ctttgccgct cgccgcctcg ggctcacggg aggactgaaa tacatcgagc gcgaaatgca
     2821 aattgatcga gaaccggaga ttcaaggatt ggacggatgg gacgctgtac gcttatggta
     2881 tcaatggcgc ggcggcgatg agggagcatt ggatctgctt cttcgttaca acgctgcgga
     2941 cacgagaaac ctcgaaccct tggcggaact gctctacgaa cagatggtgt cacggttcgg
     3001 tccgccctcg gtcggcccca gtccgcgcgc atgcgaaacc accggatcga tgatcctatg
     3061 aaacagtcac cgcgctggtt gggcgtcgga cgcacggaaa cggggcatgt ccgcaagacc
     3121 aatcaggacg tcttcgccgc gatcaatagt tgcaattttt ggatcatcgc cgacggcatg
     3181 ggcggccacc cggcagggga tgtcgcggcg catgtggcgg tagacgtggc cactcagacc
     3241 gcgaagaccc tcctctcaac ccgtgaacaa ccgtcccaac ccccagacaa gagtttaatt
     3301 gaaaccgtca ttgcagccaa tcgggccatc catgacaaaa tccgtgaaga ttccgctctg
     3361 aaaggaatgg gaaccacggt cgtcgccatg accattccac cgggcccgac acccgtcgcg
     3421 catgtggccc atttgggcga cagccgcgcg tatcgattgc atgccggcgt gcttactcaa
     3481 gtcacgcgag atcatacgtt agtggaagtt ctactgcagc gtggattgat caacgaagcg
     3541 accgcgcgtg cgtatccgga tagacatgtg ttggcaaagg gactcggcat gggcctggag
     3601 atgacaccgg atctctcgtc gactcctctg gcgcctgatg acaccctgtt gttgtgcagt
     3661 gacgggttga cgaaaatgct cagcgatgcg gagatcgggt caattttggg ggctactcat
     3721 gcgaatcccc gacgagcctg tcatgagttg gtcgaacagg ccttgcgggc aggcggggaa
     3781 gacaatgtca ctgttatcgt gtgcgcatat acgacgccgg cgaaggtcgg aagtctggcc
     3841 attgacaaga ctccaccagc catgtagcct cataatttag ccacaagctg cttctgcgca
     3901 ccatccgtac gtacgagttc tggagatcca aaacgtcatg aatcgcggtc ggtgtgtgtg
     3961 ggtcctgagc gccatcgtta tgttggcatc agtctccagc ggacgcgcgg ccgaggatca
     4021 gagcgccgaa gctactgtac gagccctggt gcgagcgaac gccgaaaaag acatggagac
     4081 catgtcacga ctgatggctc atgacgccga cattaccagt tacaccatcg ggggaagaaa
     4141 atacgtcggc tgggtcgatc ttgaaaggga catggaggag gagttcagat cagcggcaat
     4201 catcgaaatc cccatccggg aactcaaagt gtggacgaga ggcgatctgg cctggtatgc
     4261 catggaagtc gactacatcc gcacggtaac ggcaggcagc caaccgcagc gcgccctcct
     4321 tccacttcgc gagacaggcg tgttggagcg acgagacggg caatgggtac tgctgtcgtg
     4381 gcacgaatcg tttcgcaatg ccggtggtgc agtggccttg aacgagcatg cctccgtgac
     4441 gctggcaaca tcgaacaatc atctcgcgca aacggattta agcggcgaat gggaaatatt
     4501 ggaagtggaa gacgacaaac gctaccgagc cacgctggat aaggccggca acgggccgta
     4561 tacccaacac gggggacggt tcgtcaccag ccggttttct gaccgtctct ggcaaggcac
     4621 ctggcagcag ccgggcaatg accgtgaagg cggtttcgag attctgcttt cggaagacgg
     4681 caaggaggcc aaaggcgtct ggtggtatac cagggtcggc acccataaaa atatcccgcc
     4741 ccgcgaacac ggcggcacct atcagtggaa acggatcact ccggtcccca ccgccccttg
     4801 atgagccagc ggtcggatca tacggggcga gtgcgagggt tgcagcggac tccagccacg
     4861 agtctttcat tatcttttgg aggaaccatg cgtgtgcttc tggcagtgct gttgtgtctg
     4921 tggtctgcgc ctgtgatggc ggacgggggc catgatcacc atgcggtgcc gactttaaaa
     4981 aaccagacga cgacggttgt gaccatcaaa gacaatgccg tcacgctcac attcggaccg
     5041 atcgacctgc cttccggcca cgagggcgac ctggcggctt ccatgccgaa acatgtgttt
     5101 cagctcccga aggatatgta catggtgggg ttcaaatcag cggtcttcac caaagacggc
     5161 acgccgctgc ccagaaacta ccttcaccat atcttgatgc tcaataacga caagccgagc
     5221 gtttcctgcc cgggcgagcc gctgtttttc agcggcgcgg gattagagat gaccgaagca
     5281 aaattccccg acggatatgg agtgaaactg ggaaagggtc agcgcctcat gtcggtcgtg
     5341 gcgttttatc acaaagcgcc gcccaccagg aacgtgatgg cgagcttcac gatgtatatg
     5401 gcgccggaga acacgcccgt caaagagttg gaagtctatc aggtcggtgt gaatgtagtc
     5461 tgttacagta aatttgccca acggggacct gatcaaacgg atgaaggaat tgaaatcaag
     5521 ccgggcgtcc aagtgcatag cgccccggta aaattttcca tggatggctg tgtcaagttt
     5581 gcctatccgc atggccacga tgaactgtta ctcatcgctc tcgagaataa aaccagacag
     5641 cagaccctct tgcgtaccgt gccggatgtc aggctcgacg gcacattctt ggaatttcaa
     5701 ccgcatcagg tttacaagga tccccaaggc ttttccgtca gcaaggaaga agactatgag
     5761 atggtcatgg tccaccatca cccgctgcag aaacaggatc cacagcacgg catgggcaat
     5821 tatctgctct acatgacgcc gggaacctgt ccgactcaaa aaacggccag tgccaattaa
     5881 tcatgagatg agggccccca acgaggagcc cccgacggcc aatgagcgct gtgcaatatt
     5941 gttcgtgaat tgagtgcgac tggtccttcc cctacgaaag taggagccat ttcccctaga
     6001 gccaggctgg gtttcttcct cgcacctgtg ctaagcacat accgaccccg agcagcagaa
     6061 attcgtcttc ccgcgccgag acaaggtcgg ccttttcaag cgcaggatgg tgacctctcc
     6121 gctctggaac cgtgtttcct ttgacacgat ctacgcattg gaaatcggtc gatgccacag
     6181 agcgagccta taacaatcct cgtcatcaat gaacacgccg aagagatcaa agtcgttacc
     6241 attagcctcc gtggcttttt tcacgacgtc cgcattgacg cagcctattc cactgaggag
     6301 gcctcccgta tggcctcgac aacgactagc ggatgggccg tcgtcctgat agatgaaggc
     6361 agccttccga gcgggcggtc gacactgctc cacgacctca aacgacagat tcctcacgcc
     6421 tctgtcatat tgcagagcac gcgcactgac gccgcggctg ccctggaagc gcttgaaaat
     6481 ggggcggact tcttcctgaa caagcagtct ccggcctttt tgacagaatt gcttttctgt
     6541 gccaaggaag ccttcgacaa gcgcgacttg agggtatcgg ccgaccgggc gcaagttcgc
     6601 cattcttggc tcgtcgactc actcactgag attgtgtatg aactcgatgc cgacggccgc
     6661 tttcttacgc ttggccggag tctgtccgtc ttgctgggtt atcatcccga cgaactgatt
     6721 ggtcgtccgt attacaccct ctttccacag gatcaacagt cgctcgcgcg atttcgcttc
     6781 aatgaacgac gtaccggtgt ccgggcctcc actggcgtgg agttgacgct tctaggaaag
     6841 ccgacagcgg ccggtgagtc gcaggtgacc gcagccatta gcgcacgggg ggtttacgac
     6901 ctctcccgac gattcgtagg aacggtaggc cttatccgcg aggtccgtga gcagacgaag
     6961 gcgcagccgc tacggcacga aattggccct caacaacgga ctgatgacct ccacgcgctc
     7021 gtccgccaga tgagggatct ggcgcaacgc ctccaacagc cgctgtccac ccttctacaa
     7081 gaggcacaac aactttcaga ccggctgcga gaattcggcg tcattgaacg cctcggtgca
     7141 ttgatcgagc acgcagccgc ctccgcgcaa ttgggcgaac aattgtcgcg cgccgccgaa
     7201 gaagctccgc gaacagatac gatcaatcgc ttgctcgaag acctcctcac gtcggcattc
     7261 tcagactacg ctgccggcgg gttagtgtcc gatttgactt cggctcttcc cccctacgag
     7321 ggtgaccgag aaaaaacgac tcggctcctc cgacggctgc tggtgtatgc gggttgcttc
     7381 ctcacggcag tcggtcgtgc tcatgggctg gcagtcaaaa cgagtgtgga taccccgccc
     7441 gcattcacgg atgcgccaac cctgtttccg ctttcccagt cccgccaggt ccaggtggac
     7501 atcactgaaa ccgagcacac ggtcgttgca cccgcatggc ccactcgtga cagtccgctc
     7561 gatgctgcag aactgtttca actagcccga gaactcg
//
LOCUS       DASTQY010000130         5697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1713592, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000130 DASTQY010000000
VERSION     DASTQY010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713592"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /gene="kdsA"
                     /locus_tag="VFR82_04140"
     CDS             <1..735
                     /gene="kdsA"
                     /locus_tag="VFR82_04140"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEU4500819.1"
                     /translation="QLVIETAGRIAEIAKALGVPYVFKSSFDKANRTSITSFRGPGLE
                     QGLEVLAKVKKQIGVPVLTDVHTEIQATQAGHVVDVLQIPAFLCRQTELLIAAAKTGK
                     VVNVKKGQFLSPLEMANAVNKVEECGNSRIVLTERGSSFGYNNLVVDMRAFPILRSFG
                     YPVVFDATHSVQLPGGGGTKSSGQREFVEPLACAAAGAGVDGFFMEVHPNPNEALSDG
                     PNMVPLHQLKSLLERVMRICDAAKQN"
     gene            711..1727
                     /locus_tag="VFR82_04145"
     CDS             711..1727
                     /locus_tag="VFR82_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEU4500820.1"
                     /translation="MRRSKTKLGTKGAADKPNGGSLTEGRRVLRIEARAVQALIERLD
                     DQFDKAVELLFRCKGKVVVSGMGKSGLVGQKIAATMASTGTPAFFLHPADGIHGDLGM
                     LGRADVLIAVSNSGETQEILQLLPYVERVGIPVVSMTGRVNSTLAKNSDATLDVSVAE
                     EACPMGLAPTASTTATLAMGDALAVALLQKRGFKQEDFAQFHPGGTLGRRLLVRVRDL
                     MHGGADMPAVAESVPATAAILEITAKKLGMTTVIDAGGKLVGIITDGDLRRFIHKGGD
                     FAKVTAGELASRNPKVIRPDDLAAKAVEQMERFSITTLVVAEGDRKMFGVIHLHDLLK
                     NGIV"
     gene            2048..2644
                     /gene="pgsA"
                     /locus_tag="VFR82_04150"
     CDS             2048..2644
                     /gene="pgsA"
                     /locus_tag="VFR82_04150"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU4500821.1"
                     /translation="MNRVLEAWKAVGQESLNLPNFITLVRILLIPVFVMLFFTPTPDR
                     SLSAAVVFVVAAITDLLDGYIARRTGQVTKLGKLLDPIADKLLVLSALILLVNIDRVS
                     ALVAILVIAREVAVTGIRAIAAGEGMIIVAETTGKYKMALQVVAITMLILEGTVLAEL
                     GNLHLAGSVTLYLSLVLGYVSGGLYVWSFWKQVVAKGL"
     gene            2654..3256
                     /gene="plsY"
                     /locus_tag="VFR82_04155"
     CDS             2654..3256
                     /gene="plsY"
                     /locus_tag="VFR82_04155"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU4500822.1"
                     /translation="MQEEPVCILLAMFGFLLGSIPFGIVVSKALNLSDPRTVGSKNVG
                     FTNVLRVSGTRAGVLTLLGDMGKGWVMGWAAMQWLTTESFIMVVAVSPILGHLFSPFL
                     AFRGGKGVATALGAVLGLSPSIGTLLLLIWIGAVAIWRYSSGGALAAFGLFPVVAIVN
                     EQRQEFLIFAIFVSALVWVKHKDNIVRLWRGTEGKIGQRA"
     gene            3531..3782
                     /locus_tag="VFR82_04160"
     CDS             3531..3782
                     /locus_tag="VFR82_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500823.1"
                     /translation="MERIADVLVRRGMSAPAMVFLESIGPMSFLGSQALHFLTPIIDC
                     AFNAKEIEQVARLLEHRETISRLIAVIEARSSVQRASAQ"
     gene            3779..5608
                     /locus_tag="VFR82_04165"
     CDS             3779..5608
                     /locus_tag="VFR82_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HEU4500824.1"
                     /translation="MTLSSSTPPVIDRPLNVIVATDCGSTTTKAILIEKVGDEYRQTY
                     RGEAPTTVEAPFEDVTRGVLNAIAEIEELSKRKILDGDKIITPCRDAHTGVDLYISTS
                     SAGGGLQMMVTGVVQNMTGESAQRAALGAGAIVIDVLASNDGRLPHEKIERIRSMRPD
                     MILMSGGTDGGAVTHVVEMAEYIAAAEPRPRFGITYKLPLIYAGNKEAQPQVRKILGE
                     KTALEVTENIRPVLEREQLAPARNKIHDLFLEHVMQQAPGYKKLIEMTGAPIMPTPAA
                     VGLIMETIARREHLNLIGVDIGGATTDVFSVFDGVFNRTVSANLGMSYSISNVLAEAG
                     LANIMRWVPFTIDEQTLRNRIKNKMIRPTTIPQTLDELQIEHAISREALRLALIHHKS
                     LATGLKGIQQERTISDVFEQQASGRTLIDMLKLNLIVGSGGILSHAPRRIQSMLMMVD
                     AYEPLGFTMLSVDSIFMMPHLGVLSTINEQAATDVFVRDCMIYLGTCIAPIGQGKEGD
                     LCADYEIVFPDGRIVKDQLAFGELRLFALEPGMKAKMTVRPAKSVHLGAGAGAAMTAE
                     VMGGVAGLLLDGRGRPLQIPSEQFARVAALKKWFNAVGLYPQE"
BASE COUNT         1181 a         1560 c         1753 g         1203 t
ORIGIN      
        1 cagttggtaa tcgaaacagc cggtcgcatt gctgaaatcg ctaaagcgct tggcgtacct
       61 tacgtgttca agtcgtcgtt cgacaaagcc aatcggactt caatcacgtc gtttcgcgga
      121 ccggggctgg agcagggcct cgaggtgttg gccaaggtga aaaagcagat cggagtgccg
      181 gtgttgaccg acgtgcacac ggagatccag gcgacccaag ccggtcatgt cgtggatgta
      241 ctgcagatac cggcgttcct ctgccggcag acggagttgt tgattgccgc tgcaaagaca
      301 ggcaaagtcg tgaatgtaaa aaaaggacag tttttgtcgc cgctcgaaat ggcgaacgcg
      361 gtgaataagg tcgaagagtg tggaaacagc cgcatcgtgt tgactgagcg tggctcatcg
      421 ttcggctaca acaacctggt cgtggacatg cgggccttcc cgatcctccg cagtttcggg
      481 tatccggtgg tgttcgacgc aacgcacagc gtgcagttgc cgggtggggg cgggacaaaa
      541 tcgagtggac agcgcgagtt cgtggagccg ctggcctgtg cggcggccgg ggccggcgtc
      601 gacggatttt tcatggaagt gcatccgaat ccgaatgaag ccctctcgga tggaccgaac
      661 atggtgccgc tccatcaact gaaatcgctt ctcgaacggg tgatgcggat atgcgacgca
      721 gcaaaacaaa actaggcacc aagggggcag ccgacaagcc gaacggcggg agcctcactg
      781 aaggccggcg cgtcctgcgg atcgaagcgc gcgccgtgca ggccctcatc gagcgactgg
      841 acgatcagtt cgacaaagcc gtcgagctgc tgtttcgttg taagggcaag gtggtggtgt
      901 cggggatggg caagtcggga ctcgtcgggc aaaagattgc ggccactatg gcaagcacgg
      961 gtacgcccgc ttttttccta cacccggctg acggcatcca tggagacctt ggtatgctgg
     1021 gccgcgcgga cgtgctgatc gccgtttcca acagcggcga gacgcaggag atcttacagt
     1081 tactgcccta cgtcgagcgg gtaggtattc cggtggtctc gatgacgggg cgcgtgaact
     1141 ccacattggc aaagaacagc gatgcgaccc tcgacgtatc cgttgccgag gaagcgtgcc
     1201 ccatggggtt ggccccgacg gccagcacga ccgccacgtt ggccatggga gatgcgcttg
     1261 ccgtcgccct cttacagaaa cgagggttca agcaggagga ttttgcgcag tttcatcccg
     1321 gtggcaccct tggcaggcgg ctgctcgtgc gagtgcgaga tctcatgcat ggcggagcgg
     1381 acatgcccgc ggttgctgag tcggtgcctg ccactgcggc aattctggag atcacggcga
     1441 agaaactggg catgacgacg gtcatcgacg ccggcggcaa gcttgtcggc atcatcaccg
     1501 acggggattt gaggcggttt atccacaagg gcggcgactt tgcgaaagtc acggcgggag
     1561 aactcgcctc gcgtaacccc aaagtcatca ggcctgatga tcttgcggcg aaagccgttg
     1621 aacaaatgga gcggttttcc attaccactt tggtggttgc cgagggcgat cgaaagatgt
     1681 tcggtgtgat tcacctacat gatttgctta agaacggcat cgtgtaggaa ccatgcacca
     1741 gcgccctgca tcattttcgc gtgctcaacg attcatgaaa tgacccaacg ccgactagtc
     1801 gcttgcatcc ctaattccga acagtgctat gggaaaacga ttgcgaagca atcgatgggt
     1861 tgggcgggtg caaactgtga aacgattgcg aagcaatcgg tgggttgggc gggtacaaac
     1921 cgtgaagcga gtgcagcatc ggtggagcgg gtggtgcaaa agaatcacca tggtggacgg
     1981 gtgagtagtc acccgcttga gagttaacga gttcatcctt tcgaaaagag ggagcgacta
     2041 catcgcgatg aatcgtgtcc tcgaggcatg gaaggcggtt gggcaagagt ccttgaattt
     2101 gcccaatttc atcacgctcg tccgcatttt gctgatccct gtgttcgtga tgctattttt
     2161 tactcccacg ccggaccgtt cgttgagcgc ggcggtcgtc ttcgttgtgg cggctatcac
     2221 ggatctgttg gacgggtaca tcgcccgtcg cacgggccag gtcacgaaac tcggcaagct
     2281 gcttgacccg atcgccgaca agctgctcgt gttgtcagca ttgatcttgc tcgtcaacat
     2341 cgatcgcgtc agtgccttgg tggcgatcct tgttatagct agagaagtgg ccgtcaccgg
     2401 catccgcgcg atcgcggccg gggaaggcat gatcatcgtg gccgagacga ccggcaaata
     2461 caaaatggct ctgcaggtgg tcgccatcac gatgttgatc ctcgagggca ccgtcctcgc
     2521 agaactcggc aatctccatt tagccggcag cgtgacgctg tatttgtcgc tcgtgctcgg
     2581 gtacgtgtcg ggcggcttgt atgtgtggag tttctggaag caagtggtcg ccaaaggtct
     2641 gtgaagacta tcgatgcaag aagaacccgt ctgtatcctg ctggcgatgt ttggtttcct
     2701 cctgggttcc attcctttcg ggattgttgt ctccaaagct ctcaatcttt ctgacccacg
     2761 caccgttggc agtaagaacg tggggttcac gaacgtcctg cgagtgtccg gtaccagggc
     2821 cggagtcctg acgcttctgg gtgacatggg gaaagggtgg gtaatgggct gggccgcgat
     2881 gcagtggctg acgaccgagt ccttcatcat ggtggtggct gtgtcgccga ttctcggcca
     2941 tctcttctcc ccatttcttg ccttcagagg cggcaaagga gtagctacgg ccctgggggc
     3001 agtgctgggg ctttcgcctt ccatcggcac cttgctgctg ctcatctgga tcggagcggt
     3061 cgcgatctgg cggtattcgt ccggcggtgc gctcgccgcc ttcggcctct tcccggtggt
     3121 cgcgattgtc aacgagcagc ggcaagagtt tctgatcttc gccattttcg tcagcgcgct
     3181 ggtctgggtc aaacacaagg acaacatcgt acgcctctgg aggggaacag aaggaaaaat
     3241 tggccaacgc gcgtaacccc cgccctcaag cagaaacgaa catccgtatc gtgaccacgg
     3301 tatgtcgcgt cggcgcgatt ctgaagccgt tccgcagccc gtcaaattga ctctcctcgt
     3361 agccgtccat ataatgcgtt gacctccgtt tctcttgtcc tatcaagcct atgcgcccct
     3421 tgttcaagga tcatctcacc aagagactgc gcgacttgcg cacgggcctc cgacatgctt
     3481 tttcaactca ctccgacagt cacccgctca gcacggatga tgtcgctctc atggagcgca
     3541 tcgccgacgt gcttgtcagg cgcgggatgt cggctcctgc aatggtgttt ttggaatcca
     3601 taggaccgat gagctttttg ggcagtcagg cgcttcattt tctcactccc atcatcgatt
     3661 gcgcattcaa tgccaaggaa atcgaacagg ttgcgcgtct actcgaacac cgtgagacga
     3721 tctcgcggct gatcgccgtc atcgaagcca ggtcatcagt gcaaagggcg tcggctcaat
     3781 gacgctttct tcctcgaccc cgcccgtcat tgatcgtcct ctcaacgtca tcgtggcgac
     3841 cgattgcggc agcacgacga cgaaggccat cctcatcgaa aaggtcggcg acgaataccg
     3901 tcaaacgtac cgcggcgagg caccgacaac tgtcgaagcg ccttttgaag acgtcactcg
     3961 cggtgtgctc aacgcgattg cggaaattga agaactttcc aagcgcaaaa ttctggacgg
     4021 cgacaagatc attacgccct gccgggatgc acacaccggc gttgacctct acatctcgac
     4081 cagcagcgcc ggcggcggct tgcaaatgat ggtgacgggc gtcgtgcaga acatgacggg
     4141 agaaagcgcg caacgcgccg ccctcggtgc cggcgcgatt gtgatcgacg tgctggcctc
     4201 gaacgacggc cggttgcctc atgagaagat cgaacggatt cgctcgatgc ggcccgatat
     4261 gattttgatg tcaggcggga cggacggcgg cgccgtgacg catgtcgtcg aaatggcgga
     4321 atacattgcc gcggccgagc cgcgtccccg cttcggcatt acctacaagt tgccgctgat
     4381 ctatgccggg aataaagagg cccagcctca ggtgaggaag attctgggtg aaaaaactgc
     4441 cctggaagtg acggagaaca tccgcccggt gttggagcgg gagcagttgg ctccggcccg
     4501 gaacaaaatc catgatttgt ttctcgagca tgtgatgcag caggcgcccg gttacaagaa
     4561 actcatcgag atgaccggcg caccgatcat gccgaccccc gcggctgtcg ggcttatcat
     4621 ggaaacgatc gccaggcggg agcacttgaa tctcatcggc gtcgacattg ggggcgcaac
     4681 caccgacgtc ttctcggtgt tcgacggggt attcaatcgg acggtcagcg cgaacctcgg
     4741 aatgtcgtac agcatttcca atgtgctggc agaggccggg ctggcgaaca tcatgcgttg
     4801 ggtgccgttc acgatcgatg aacagacgct gcgcaaccgg atcaaaaata agatgatccg
     4861 gcccacgacg attccccaga cgctcgacga gttgcagatc gagcacgcga tttcccggga
     4921 ggcgttgcgg ttggctttga tccatcacaa atcgctggcc accggattga aaggcattca
     4981 acaggagcgc accatctcgg acgtgttcga gcagcaggca tcagggcgca cgctgatcga
     5041 catgctgaaa ttgaatctga tcgtcggaag cggcggaatc ctttctcacg ccccacgccg
     5101 aattcagtcg atgctgatga tggtcgatgc ctacgagccc ttgggcttca ccatgctgtc
     5161 ggtcgacagc atttttatga tgccgcactt gggcgtgctc tcgacgatca acgagcaagc
     5221 cgcgactgat gtgtttgtcc gagactgcat gatctatctc ggcacgtgca tcgcaccgat
     5281 cggacagggc aaggaaggcg acctctgcgc cgactacgag atcgtttttc ccgatggtcg
     5341 aattgtgaaa gatcagttgg cgtttggcga gctcaggctg tttgcgctgg agcccggcat
     5401 gaaagccaaa atgacggtac gcccggcgaa gtcggtccat ttgggggcgg gagccggggc
     5461 ggccatgacg gccgaggtca tggggggcgt tgcgggcttg ttactcgacg gtcgaggccg
     5521 tccattgcaa ataccctccg agcagtttgc acgggtcgct gccctgaaga aatggttcaa
     5581 cgccgttggt ttatatcccc aggaatagcg atgatgccac gcggcatgag gtccaggtac
     5641 tcaagaagct agcttgtgaa ccaccggttt cgattccatg ctggggaaga aaatatt
//
LOCUS       DASTQY010000131         5584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1659355, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000131 DASTQY010000000
VERSION     DASTQY010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659355"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /gene="rplT"
                     /locus_tag="VFR82_04170"
     CDS             complement(<1..253)
                     /gene="rplT"
                     /locus_tag="VFR82_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU4500825.1"
                     /translation="MPRAKGGPKTRARRKKRLKLAKGQYGAKSRLFRSATESVDKGQQ
                     YAYTGRKNRKRDFRQLWIARISAATRAHGLTYGRFISALK"
     gene            complement(289..483)
                     /gene="rpmI"
                     /locus_tag="VFR82_04175"
     CDS             complement(289..483)
                     /gene="rpmI"
                     /locus_tag="VFR82_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU4500826.1"
                     /translation="MKMKSHSGASKRFHRTGTGKFMRKRAGKRHILTSKNRERKRRLS
                     GSVVVDETKAAALKRLLPYT"
     gene            complement(516..1007)
                     /gene="infC"
                     /locus_tag="VFR82_04180"
     CDS             complement(516..1007)
                     /gene="infC"
                     /locus_tag="VFR82_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU4500827.1"
                     /translation="MNREIRVREVRVIGPEGEQLGILPTPEAFKQAQEGGYDLVEVAP
                     TSVPPVCRIMDYGKYKYELSKKDHQSRRHQKSTQVKEIKLRPRTDKHDLEIKIRQIKG
                     FLEEGNKTKVVLTYRGREMANQEMGRVVMNSVISELSEAGTIEYAPRMEGRSLIMIVA
                     PKN"
     gene            complement(1036..2970)
                     /gene="thrS"
                     /locus_tag="VFR82_04185"
     CDS             complement(1036..2970)
                     /gene="thrS"
                     /locus_tag="VFR82_04185"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU4500828.1"
                     /translation="MPKVTLKNGKSFDVPPGYTIAQALAAGGITVGPEVLAALVNGKP
                     TDLSASLTADATIDPLGFDTAEGREIYRHSSTHIMAQAVKELFPSAQLTIGPALEDSF
                     YYDFAYDRPFTPEDLENIEQRAREIAKRKLAVTRRDLTKQEALELFRARGEHYKVELI
                     EGFPDREPISIYTQGEFVDLCRGPHVPSTGHVGAFKLLTTAGAYWRGDERNPMLQRIY
                     GTSFPTKAELDAHLAKLEEIKRRDHRKVGKELDLISIQDEIGPGLVLWHPKGAVIRLL
                     IENFWREQHLKDGYELVYSPHVARLDLWKTSGHVDYYRDNMFAPMKLEGSEYQLKPMN
                     CPYHIMIYKSHLRSYRELPIRYGELGTVYRYERTGVLHGLLRVRGFTQDDAHLFCRPD
                     QIEAEVSRVLDFTFFILRTFGFMEFEVFLSTRPNESVGAEEHWTLATSALEAALKSRN
                     ISFHLDPGGGAFYGPKIDIKIKDALGRSWQCSTIQIDFNNPERFELSYIGEDGKSHQP
                     IMIHRALMGSIERFFGILIEHYAGAFPTWLAPVQAVVMAITDNQREFVTEVVAQMKAS
                     GFRVESDIRNEKIGFKIREAEKAKTPFMFVVGDREVQSRSLSVRGRSGANLGTMTVQG
                     ALDLLKTEVERAGQQLSPTS"
     gene            complement(3130..3204)
                     /locus_tag="VFR82_04190"
     tRNA            complement(3130..3204)
                     /locus_tag="VFR82_04190"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3170..3172),aa:Val,seq:gac)
     gene            complement(3339..3611)
                     /locus_tag="VFR82_04195"
     CDS             complement(3339..3611)
                     /locus_tag="VFR82_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU4500829.1"
                     /translation="MTKEELIAKMAASAGITKVAAGNALHAFTGAVTSSLKKGQRVSL
                     VNFGTFTISKRKARMGRNPRTGESLKIPAAKVPKFSAGKVLKSAVR"
     gene            complement(3701..4201)
                     /locus_tag="VFR82_04200"
     CDS             complement(3701..4201)
                     /locus_tag="VFR82_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU4500830.1"
                     /translation="MITAKDATALPKEVAKRTFDAVIIEAKPGMLNGSGVLPHHVDLS
                     RTLVITGSKPVLKRASKVVQTIARAKATVADGGPAPMSLEDYVEWKMGDFVKGMRNGS
                     GRNLHPMLISAIERPLITKALQETKGNQIQAAELLGLNRNTLRKKIQDLHIPVKRGRS
                     ARVREA"
     gene            4188..4364
                     /locus_tag="VFR82_04205"
     CDS             4188..4364
                     /locus_tag="VFR82_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500831.1"
                     /translation="MAVIIAESLNVCLNWLWISESPLSNKMLNTEEESVMKPSPEEEG
                     RIIAQSRFDVNEEM"
     gene            4656..5096
                     /locus_tag="VFR82_04210"
     CDS             4656..5096
                     /locus_tag="VFR82_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU4500832.1"
                     /translation="MKVSLRSTYGIMAAVDMAMQSGSGPIQAKSIARRQGIPARFLEQ
                     VLHAMKKAGLVSSLRGAQGGYVLSKKPSDLSVAEILEALEGPLFHGGVNGRLSPTTKS
                     DPLLAKIWTRVSLAEREVLQGITVEELAGQQRKIQEQRTVMYHI"
     gene            5105..>5584
                     /locus_tag="VFR82_04215"
     CDS             5105..>5584
                     /locus_tag="VFR82_04215"
                     /inference="COORDINATES: protein motif:HMM:NF015425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500833.1"
                     /translation="MSQLSTKSIAVQTQPIPPAIQEEIETFEQEAQRMLAGDIAGDLF
                     KPFRLQYGIYGQRQAGVQMVRIKIPFGGISSNQLRRIAELSERYTTGVGHVTTRQDIQ
                     LHFAELKDIPAIMRGLAEVGLTTREACANTVRNVTGCHLAGVCQGEVFDITPYAKTVA
                     "
BASE COUNT         1175 a         1568 c         1510 g         1331 t
ORIGIN      
        1 ttttaagcgc actgatgaag cggccgtacg tgagcccgtg cgcccgcgtg gccgcgctga
       61 tgcgagcgat ccacaattgc cggaagtccc gcttccgatt cttgcgcccg gtgtaggcat
      121 actgctgtcc cttatctacc gattctgtcg ctgagcgaaa cagccggctc tttgcgccat
      181 attgcccttt cgccagcttg agtcgttttt tccgtcttgc tcgtgttttt ggcccacctt
      241 ttgcgcgtgg catgactcat actccttgtt gagctcaggt taacatctct atgtataggg
      301 tagtaggcgc ttgagtgccg cggccttggt ttcgtcaacg accaccgatc cgctcagccg
      361 gcgtttccgc tcccgattct tgctggtcag aatatgacgc ttgccggcgc gtttgcgcat
      421 gaatttgccc gtgcctgttc tgtgaaaccg tttgcttgct ccgctatgtg acttcatctt
      481 catgttcgca tcctttggtt aagcgttcgg cctctctaat tcttaggagc gacgatcatg
      541 atcaagctgc gcccttccat ccgcggcgca tactcgatcg taccggcttc ggatagctca
      601 gagatcactg agttcatgac gacccgtccc atttcctgat tggccatttc tcgcccccga
      661 tacgtcagga cgaccttcgt cttgttgccc tcctccagga agcctttgat ctgtcggatt
      721 ttgatctcca ggtcatgctt atcggtgcga ggacgcaact tgatttcctt cacctgggtc
      781 gatttttggt ggcgacggct ctggtggtct ttcttgctca gctcgtactt atactttccg
      841 taatccatga tgcggcagac aggtgggacc gacgtcgggg ccacttccac caggtcatat
      901 ccgccctctt gtgcctgttt gaaggcttcg ggagtcggga gaatgccgag ttgctcacct
      961 tccggcccga ttactctgac ttcacggact cgaatttcgc ggttcacccg cagtttgggg
     1021 acgataagcc acctctcatg atgtagggga aagttgctgc ccggcccgct cgacctcagt
     1081 cttgagcaga tccagcgcgc cttggacggt cattgtgccg aggttggcac cgctacgacc
     1141 tctcacggac agtgagcgac tttgcacttc gcggtcgccg acgacgaaca taaacggtgt
     1201 cttcgctttt tccgcttcac gaatcttgaa tccgatcttt tcgttccgaa tgtcggattc
     1261 cacacgaaag cctgaggctt tcatctgtgc aaccacttct gtgacaaatt cacgctgatt
     1321 gtctgtaatc gccatgacca ccgcttgcac cggtgctagc catgtcggaa aggcgccggc
     1381 atagtgctcg atgagaatgc caaaaaaacg ctcgatggac cccatgagcg cacggtggat
     1441 catgatgggt tgatggcttt tcccgtcctc gccgatgtaa ctgagctcga accgttccgg
     1501 attattgaaa tcaatctgga tggttgagca ttgccaggat cggccgagcg catccttgat
     1561 cttgatgtcg attttcgggc cgtagaatgc gcctccaccg gggtcaagat gaaaagagat
     1621 gttgcggctt ttcagcgccg cttcgagtgc gctggtggcg agcgtccaat gctcttctgc
     1681 gccgactgat tcgttcggac gagtcgaaag aaacacctcg aattccatga atccaaacgt
     1741 ccgcagaatg aagaacgtga agtccagcac gcggctgact tcggcctcta tttgatcggg
     1801 tcggcaaaac aaatgcgcat catcctgcgt gaaaccacgt acgcgaagca ggccatgcag
     1861 cacgccggtg cgttcgtagc ggtacaccgt ccccaactcg ccatagcgga ttggaagctc
     1921 tcgatagctc cgtaaatgcg atttgtagat catgatgtga taggggcagt tcatcggctt
     1981 tagttgatac tcgctgccct caagcttcat cggcgcgaac atgttgtcgc gatagtagtc
     2041 gacatgcccg ctggtcttcc acagatctaa ccgtgccacg tggggcgaat acaccagctc
     2101 atagccatct ttgaggtgct gctcacgcca aaaattctca atcagcagtc gaatgacggc
     2161 acccttcgga tgccacaaca ccaggccggg tccgatctcg tcctggatcg aaatcaggtc
     2221 taattccttc cccactttgc gatgatctcg acgtttgatt tcttccagct tggccaggtg
     2281 agcgtccaac tcggccttgg tgggaaaaga ggtgccgtaa attctctgga gcattgggtt
     2341 gcgctcatcg cctcgccaat aggcgccagc cgtcgtcaaa agcttaaagg cgcccacatg
     2401 accggtcgac ggcacatgtg ggccacggca aaggtctacg aactcaccct gcgtataaat
     2461 ggaaatcggc tctcgatccg gaaagccttc gatcaattcc accttataat gctccccacg
     2521 agctctgaaa agctctaatg cctcttgctt cgttaggtcg cggcgggtga cggcaagctt
     2581 gcgcttcgcg atttcgcgag cgcgctgctc gatgttctcg agatcttcgg gcgtgaacgg
     2641 ccgatcgtag gcaaagtcat aataaaagct gtcttctaaa gccggcccga tcgtcagttg
     2701 agcggaggga aaaagttctt tcacggcttg cgccatgatg tgtgtgctgc tatggcgata
     2761 gatttcccgg ccttcggcag tgtcgaaccc aagtggatcg atggtcgcat ctgcggtcaa
     2821 agaggcagac aagtcggtcg gtttcccatt gaccaaggcg gccagcacct cgggtccgac
     2881 agtaattcca ccagcggcca gggcttgggc aatagtatag ccagggggga cgtcaaagct
     2941 cttcccattc ttcagggtga ctttcggcac attcgtctcg ctatggctcg tgggcgcaga
     3001 ccaccttccc ttgagaatcc ggttgaacgc cgaaatctca cgaggtggga aacatcctgt
     3061 ttctcagcag gatacaaaaa tacaaaaggc accccaaccg cagcaggcgg tcagatgccc
     3121 tcggcgagat ggtaggcgcg gacggtcttg aaccgtcgac ctctaccgtg tcaaggtagc
     3181 gctctccccc tgagctacgc gcctaacgtc cgagcgcgca tgctaatata gccccgccca
     3241 aggtgtcaag aaatgcaaag agaagggagc gtgcttcgat gtgagcacgc cgccttctct
     3301 ttgcgcgatc cggcgcgtcg tatggggtga tccggaggtt accggacggc cgacttcaaa
     3361 actttacctg ccgagaactt cggcactttt gcagcgggga ttttgaggct ctccccggtc
     3421 cgtggattcc gtcccatcct ggccttgcgc ttggaaatcg tgaaggtccc gaagttgacg
     3481 agtgatactc gctgcccttt tttcagagaa gaggtgacgg ctcccgtaaa cgcatggagc
     3541 gcatttccag cggccacctt ggtaatcccg gcagacgctg ccatctttgc aatcagttct
     3601 tctttcgtca tcatcgccct ccttgatata ggttagaaat caaccgccac acttgctgcc
     3661 atgcagggtc tcctctcctg ctgaatccga ggtccggacg ctatgcctcc cgtacgcgcg
     3721 ctgaccgccc tcgcttgacg ggaatgtgca gatcctggat ctttttcctg agggtgttgc
     3781 ggttcaagcc aagcagctcc gccgcctgga tttggttgcc tttggtttcc tgcaaggctt
     3841 tggtgatgag gggccgttca atggcggaaa tcagcatcgg atgaagattt cgtcccgagc
     3901 cgtttcgcat gcctttcaca aagtctccca tcttccattc cacatagtct tcaaggctca
     3961 tgggcgctgg cccgccgtcc gccaccgttg ctttggcgcg ggcgatcgtc tgcacgacct
     4021 tcgaggcccg tttcagcacc ggtttggatc cggtgatgac aagagtgcgg gacaggtcga
     4081 cgtgatgagg tagtacgccc gatccattga gcatgccggg cttggcttca atgatcacgg
     4141 cgtcaaatgt gcgcttcgcc acctctttcg gcaatgcagt cgcatctttg gcggtgatca
     4201 tcgccgaatc cttgaacgtt tgcctgaact ggctttggat ttcggagtcg ccgctgagca
     4261 acaaaatgct gaacaccgaa gaagagagcg tcatgaagcc atcaccagaa gaagagggcc
     4321 ggattattgc ccagagccga tttgatgtca atgaagaaat gtgagcacga gatgatttgt
     4381 tacggcaacg gtgctgctaa ataatcgtga cgccggatcg cagcgtctct gtggtggttg
     4441 tcgacttcac gaacacctga tgcacgatta tgatgttccg caaagcaatg tcgacatcgc
     4501 tgcattggtc ttccagccgg agtgatgatc attccatcgt atctttcgca tacgacaagg
     4561 acgataaatt gggggggcag ccccacgcat tgacagcaca gaacaaacca gagtataaat
     4621 tagcttccgt ctaattccga ccggaatagt gtggtatgaa agtatcgctt agatcaactt
     4681 atggaattat ggctgcggtg gacatggcca tgcagtccgg ttctggtccc attcaggcca
     4741 aatctatcgc caggagacag gggataccag caagattcct tgagcaagtg ctccatgcca
     4801 tgaaaaaggc cggtttggtg tcgagtcttc gaggggccca aggcggctat gttctgtcga
     4861 agaaaccctc cgatctctcc gtcgccgaaa ttttggaggc acttgaaggc ccgctatttc
     4921 atggaggagt gaatggacgg ctgagtccga cgacaaaatc ggatcctctc ctggccaaga
     4981 tatggactcg cgtgagtcta gcggaacggg aggtgcttca agggatcacc gtcgaagagc
     5041 ttgcgggaca acagcgcaaa atccaagagc aacgtactgt catgtaccac atttgaggtc
     5101 agccatgagc cagctctcta ccaaatcaat agccgtccag acgcaaccaa tccctccagc
     5161 catacaggag gagatcgaga cattcgagca agaagcgcaa cgcatgctgg ccggtgatat
     5221 cgccggtgat ctcttcaagc cgttcaggct tcagtacgga atctatggcc aacggcaggc
     5281 cggcgtacaa atggtgcgca tcaagattcc tttcggcggt atctcgtcca accaactcag
     5341 acggatcgcc gaactgtcgg agcgctatac gaccggcgtc ggccatgtga cgacccgcca
     5401 agacatccaa ctgcattttg cagaactgaa agacataccc gctatcatgc ggggtctggc
     5461 tgaagttggt ctgacgacta gagaagcctg tgccaacaca gtccgaaacg tcactggctg
     5521 ccacttggca ggggtctgcc agggcgaagt gtttgatatt accccctatg ccaagacggt
     5581 ggcc
//
LOCUS       DASTQY010000132         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1547655, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000132 DASTQY010000000
VERSION     DASTQY010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547655"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFR82_04220"
     CDS             complement(<1..416)
                     /locus_tag="VFR82_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEU4500834.1"
                     /translation="MIVRKTFVPVALTATFLVAGWLSAPVFGDRIFAQQDGKAPVTMD
                     KAFPNSNKCKRCHERVFEEWETSPLSRSIHSPAFRASLDAFLSSPAGKDKALCFRCHA
                     PHVREFPDQVQLFIDQAKSGDPQMDGVACSQCHLIK"
     gene            complement(439..1761)
                     /locus_tag="VFR82_04225"
     CDS             complement(439..1761)
                     /locus_tag="VFR82_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEU4500835.1"
                     /translation="MRFRRMLTKRRIAATCIVLLLLGVTAFYQEGAVAQKADGQALNG
                     QAPDGWVGEIERVFIRSEECKQCHDRHYEEWKGAREQTPDLKTFGRVDAALLHGTSLE
                     SPVFRTVLGVWLQTNPTAEERKRCLSCHVPATTVFPQHAEKITAQILSGKPQIEGIGC
                     ASCHLIKSVETTMGPPPTFKIEPGKTMHGPYADPEENLVHPAVQSPLFRGANYCASCH
                     FDKVKDVTQKDLPGEILQGTICQDCHMEQSTGSSTSKRGAMTRSIGRHYFRGVVIPGI
                     LLKNRNLQAEWMPRIDIDSTKSAGKVEGTALVKVGSLPHSFPDGDPVLKQFMLAIAVK
                     DAQGKVLAQETKQFGLPYDKILRGPIPDPFIKGGNTRKVPFSLAVPGGAAASVEAVLS
                     YSLIPTPEPALKDKYLATLKDDKEREKAKKILDEYTQPRMLTYRAKTL"
     gene            complement(1957..>2451)
                     /locus_tag="VFR82_04230"
     CDS             complement(1957..>2451)
                     /locus_tag="VFR82_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU4500836.1"
                     /translation="APTLEEHRYRGMSGTARALRWGRALVVCGILSVLLGIPLPGRTA
                     NDRPADGMTVDEAAKLGEDFGVIVGDVDEEIQKELKLQRPQGVAVFEVIGNSRADYAG
                     IKVRSVIKEIDKKEVKNMLDFGRHIKKAINECNFTVGTYEPADPGDPVGWGVNFHFVG
                     CKRD"
BASE COUNT          477 a          759 c          690 g          525 t
ORIGIN      
        1 tgcttgatga ggtgacattg ggagcaggcc acaccgtcca tctgcggatc gcccgatttc
       61 gcctgatcga tgaagagttg cacctgatcg ggaaattccc tcacgtgtgg cgcgtggcag
      121 cggaagcaga gtgccttgtc cttcccggca ggcgatgaca gaaacgcatc aagcgaggcg
      181 cgaaaggcgg gtgaatggat cgaccgggac aagggagagg tttcccactc ttcaaacacc
      241 cgctcatggc agcgcttgca cttgttggag ttgggaaaag ccttgtccat cgtgacgggc
      301 gctttaccgt cctgctgcgc gaaaatccgg tcgccgaaca cgggcgcaga cagccagccc
      361 gcgaccaaaa acgtcgcggt caaagctacc ggcacaaatg tcttacgcac gatcatgcgt
      421 tccgctgctc cacatccgtc agagggtctt cgcccggtag gtcagcattc gaggctgtgt
      481 gtattcgtcg aggatcttct tggccttctc ccgctccttg tcgtccttga gcgttgcgag
      541 atacttgtct ttcaatgccg gctcaggcgt tggaatcagc gaatagctca gcaccgcttc
      601 cacggaggcc gctgcccccc ccggcacagc cagcgagaac ggcaccttcc tcgtattgcc
      661 ccctttaatg aatgggtcgg gaattggtcc gcgcaggatc ttgtcatacg gcagtccgaa
      721 ctgtttcgtt tcttgagcca gcacctttcc ctgcgcatct tttaccgcaa tggccagcat
      781 gaattgcttg agcaccggat cgccgtccgg gaagctgtgc gggagactgc cgactttgac
      841 cagcgctgtg ccctcaacct tgccggcgga tttcgtgctg tcgatgtcga tacgcggcat
      901 ccactcggcc tggaggttgc ggttcttgag caggatcccg gggatcacca cgccgcggaa
      961 ataatgccgc ccgatcgagc gcgtcatggc gccccgcttc gaagtcgaac ttcccgtaga
     1021 ctgctccatg tgacaatcct gacagatcgt cccttgcaga atctcacccg gtaaatcctt
     1081 ctgcgtcacg tccttgacct tgtcgaaatg gcaggaggcg cagtagttgg cgccccgaaa
     1141 gagcggcgac tgcacggccg gatgcacgag gttttcctcc ggatccgcat agggaccgtg
     1201 catggtcttc cctggctcga tcttgaacgt cggaggcgga cccatcgtcg tttcgacgga
     1261 tttaatgaga tggcaggagg cgcacccgat gccctcgatc tgcggtttac ccgacaagat
     1321 ctgcgcggtg atcttttcgg catgctgcgg gaacacggtc gtcgctggca catggcatga
     1381 gagacagcgc ttccgttctt cggccgtggg attggtctgc agccaaacgc ccagcacagt
     1441 acggaacacc ggcgactcca gcgaggtgcc gtgcagaagc gcagcgtcca cccggccaaa
     1501 ggttttgaga tccggcgtct gttcccgtgc gcccttccat tcttcgtaat ggcggtcgtg
     1561 gcactgcttg cattcttcgg agcggatgaa cacccgttcg atctcgccca cccagccatc
     1621 cggcgcctgc ccattcagcg cctgaccgtc tgccttctgc gccaccgccc cctcttgata
     1681 gaaggcggtg acgccgagga gcagcagcac gatacaagtt gcggctattc gtcgtttggt
     1741 cagcattcgt cgaaaacgca tcgaacgtga gaatcctctg cggcgactag aggttgaaaa
     1801 acaccattaa cttcacagac ctactcccgc gaagtgcgag acgcttcatt ccgggcacgc
     1861 ccgcaagact gaccgatgcg agaacaaaag cctggtaaaa cgcgcgtcgc ggcgcacagg
     1921 gactgggcct ggtgagaaga ggtttttttc cacatcttaa tcccgcttgc agccgacaaa
     1981 gtgaaaattc acaccccaac cgacgggatc gccagggtcg gcaggctcgt aggtgccgac
     2041 cgtgaagttg cattcgttaa tggctttctt gatgtgccgt ccgaaatcga gcatgttctt
     2101 cacttctttc ttgtcgattt ccttgatgac tgaccggact ttgatgccgg catagtctgc
     2161 gcgtgaattg ccgatgactt cgaacaccgc cacgccttgg ggccgctgca gcttcaactc
     2221 tttctgaatc tcctcgtcga catcgccaac gatgacgccg aagtcttcac ccagtttcgc
     2281 ggcctcgtcc accgtcatgc cgtcggccgg ccggtcgttc gccgttcgtc cgggaagcgg
     2341 aatgcccagc aggaccgata gtatcccaca cacaacaagg gcccttcccc aacgaagggc
     2401 ccttgcggtg ccactcatac ccctgtagcg atgttcctcc aaagtcggcg c
//
LOCUS       DASTQY010000133        13391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1513677, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000133 DASTQY010000000
VERSION     DASTQY010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 13391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13391
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513677"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VFR82_04235"
     CDS             complement(<1..532)
                     /locus_tag="VFR82_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500837.1"
                     /translation="MTPEEREEAERLRKVAAKYGTDPTAIVGRLQLTSQYLDLPRGAQ
                     ATVSTVRVDLPFRGDFLLRTDMPFLRTSDPNRPGTPSHSGASDLAVTAGWRAYNTPEY
                     AVLIGAMSTFPTAANDALGFGKYTVGPAIATARFLPRLESFLIGLMSYQASVGGDPSR
                     RDVSLFSVTAQVNSFWA"
     gene            complement(808..1515)
                     /locus_tag="VFR82_04240"
     CDS             complement(808..1515)
                     /locus_tag="VFR82_04240"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEU4500838.1"
                     /translation="MMSKLVGALRLVSACAGITVVLVMFGAAPAFSAEAVAQQQLVDK
                     AQLTLEAFAADPQLKESLKDLNKEARALFIVPQFMRGAFIFGGAGGSGVLIARDERTG
                     KWGDPVFYNIGSASFGAQIGADVSEMVLIVRTRKGLEEFYTNDFKLGANAGMATGPVG
                     AGMSAQGLAADIISYARQKGAFAGMAIDGAFVSVSNDSNQAYYGKPVRPTDIIVKKEI
                     GNPKSLQLRAAADKLMN"
     gene            complement(1529..2041)
                     /locus_tag="VFR82_04245"
     CDS             complement(1529..2041)
                     /locus_tag="VFR82_04245"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HEU4500839.1"
                     /translation="MKGIIGCRWIAVPVLALTVSCSTMPKDFEAPRVTITDLTPKDMA
                     IFEQRFDVKLRIQNPNDQELSINGLRFDIELNAHEFASGMSGQHVAVPRFGSQVVDVE
                     VFTTLASFLRQIRDLTKREGQHVSYRLKGVAFVDAPSTFKAPFDEQGEIDLNFESLSE
                     RAPPESSKGF"
     gene            complement(2061..2492)
                     /locus_tag="VFR82_04250"
     CDS             complement(2061..2492)
                     /locus_tag="VFR82_04250"
                     /inference="COORDINATES: protein motif:HMM:NF022526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2780 domain-containing protein"
                     /protein_id="HEU4500840.1"
                     /translation="MWLRRSRFALLILTAFLHSGCAQMQSMGAADTLTKLLTNQLGVT
                     SNQAMGGVGSILSLAKERLPGMDFTALSTLIPGSESLMKTARDLGAVTGPVGDRAGLT
                     SAFSRLGMGSEMVPKFTQIMSDFVGKAGGEPARNLLATAVQ"
     gene            complement(2556..4040)
                     /locus_tag="VFR82_04255"
     CDS             complement(2556..4040)
                     /locus_tag="VFR82_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU4500841.1"
                     /translation="MKQKPVAQWPRHPTIYEINTWVWLYELSERARRPTTLGNVPKKQ
                     WDAIAAYGFDAVWFMGVWERSPAGVAISMRNDGLLQEFRRVLPDFTGEDNVGSAYCIR
                     DYRVDARLGGPEGLAHARAQLAERGMRLILDYVPNHVAPDHPWATEHPDYFIQGNSDD
                     LSTKPDDFVRVGEYILACGRDPYFPAWSDVLQLNAFNPNLRKAAIETVLSIAGQCDGM
                     RCDMAMLLMNSIFERTWGTRAGRIPQSEYWTDIIGTVKQRYPDVLFIAEAYWDLEFNL
                     QRQGFDYCYDKRLYDRLEHGPAESIRLHLCAEAAYQQKLIRFIENHDEPRAAAAFSSS
                     KAQAAAVTVSTLQGAVLFHEGQFEGRRVRLPVFLRRRPEEPSDRSLENFYRKLLKTLR
                     AEDFKKGEWQLCERTGWPDNQSYLNLVTWYVHSEQTRHLIVVNLSDSPSQGRIQLPVD
                     TRDGREVHMIDEISGTQYERSLDEIREAGLYVELAPWAFHVLTL"
     gene            4398..5483
                     /locus_tag="VFR82_04260"
     CDS             4398..5483
                     /locus_tag="VFR82_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU4500842.1"
                     /translation="MLTTPMSSSTQDQGSYAYPQRGSAAGQTQPHYQDLARLLEAIVW
                     RADATTLEITYVSEQAKRILGYPLSQWRQPDFQETHLHPEDRLKVLACYREAIRDGEN
                     HRLDYRMRAADGREVWFHDCVHAINVGARKELIGVMVDITDRKSAEACLTEMTGRLIR
                     AQEEERSRIARELHDDFNQRLALLAIGLQRLGHTFESETGVAAQVSDLYHMTQEIASD
                     VHRLSHQLHPAKLQHLGLVPAIKGLCRELSEQYGAEIDFVHRNVPNPIAQESALCIFR
                     VAQEALSNTVKHSGVKQGKLELFSNRGILHLCVSDSGTGFDPEAISAKGRLGLTSMQE
                     RVRAAGGTISVESRPSTGTRISVHLAA"
     gene            5499..6149
                     /locus_tag="VFR82_04265"
     CDS             5499..6149
                     /locus_tag="VFR82_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4500843.1"
                     /translation="MQRPRVLLADDHRMLAEACIRLLEPECEVVGTVTDGRALLPVAR
                     DLHPDVIVLDIGMPLLNGLDAGRQIKHSMPGVKLIFMTMNEDPDLTREALHIGASGYL
                     LKSSAASELPEAIRAAVRGDTYVDCRASAKLDDSFIQEGERSSTGELTARQREVLQLL
                     VEGLSMKEAARVLRVTARTIAFHKYRVMQQFRLKSNAELIQFAIEQRVTLGTPHQY"
     gene            6297..6737
                     /locus_tag="VFR82_04270"
     CDS             6297..6737
                     /locus_tag="VFR82_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4500844.1"
                     /translation="MARYRGSSSDGSPSVRVLLADDNPSMLQAARRILEPEFQVVGTV
                     NDGEAVLEAADALRPDVLILDISMGILNGLEAARLLTRTGSNARIVFLTVHQDQEFVE
                     EAFSVGALGYVVKPRLGTDLLPAVREALVGRAFVSPHLGSPKRC"
     gene            complement(6777..8048)
                     /locus_tag="VFR82_04275"
     CDS             complement(6777..8048)
                     /locus_tag="VFR82_04275"
                     /inference="COORDINATES: protein motif:HMM:NF025714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HEU4500845.1"
                     /translation="MNLEVRLPAEELAVAVDEGFPPVASREEDWNDAGTLGERDDVRF
                     LYRLVRGSFRYRMNQNHFDVWFDHVRYRVWARKGQGQEFVEGRCGHGDDPPKRLNLTA
                     HSALTWSDHWRVQSHTTFEEPVFVEPCQLAGLDLEATPFLKAVLQPRLAALGESIDRI
                     IRGRTEAKKRAETVWQKLQEPIELTHNQWLEFNPRDARVGPITSNGTLLVKSSVNLVM
                     EPKIVTGPKPKIVSMSLPPLQLAALPLEGFHLALPITVEYARINQRLEEEMLGQEFQT
                     PVGDTLKVEGVQLYGSGDKLILALRVSGGVNGSLYATGTPVFEEGLGILRFVDLDFTL
                     DTRNVLIRSANWMFHENILSSLKAHAVIDLSSQLQTLRSRLETTLTRDLGPDARLQAE
                     VLTLRPRGVYPTAGGVDVHIIADGAMWVELR"
     gene            complement(8256..8723)
                     /locus_tag="VFR82_04280"
     CDS             complement(8256..8723)
                     /locus_tag="VFR82_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HEU4500846.1"
                     /translation="MDYSRLIVIVMGLLFITSEALAAADVFVYPSKGQTKDQQEQDEF
                     SCYKWAKEQTGFDPNQPMQQAAAPPPQGGAVKGAARGAAIGAIGGAIGGDAGKGAAIG
                     AGVGAAGGAMRRRHAAEQQAAAQEKAKQAYNADVGGYKRAFGACMTGRGYTVS"
     gene            complement(8759..9379)
                     /locus_tag="VFR82_04285"
     CDS             complement(8759..9379)
                     /locus_tag="VFR82_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500847.1"
                     /translation="MKPNVVVGMCLMGVLMVPSLHAQESTLPESTLKVVRIRPNLDPD
                     KLQYILSTERKEVFERNMGLDEQQSEVFWGVYHRYEKEKDQLEAKRLRLLGTYIEKYS
                     SLTNDDVMKIVKQSSENQQADLALRKKYFHIYSKKLNPVAAARFMQLDDIVGMVTRLA
                     ILGNLPLIGDVPHAAASGSSAGEAGSTGDRDTSASGTDHPSMELTK"
     gene            complement(9488..10537)
                     /locus_tag="VFR82_04290"
     CDS             complement(9488..10537)
                     /locus_tag="VFR82_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEU4500848.1"
                     /translation="MRHPRTPWWAAGLCIVQLSSFPLAGEIAAHADSPPPGAPGTVPV
                     CSDLGPDAERLKCYDDAAARQQSQSPEPSDAGLASSARKASLMGTRWELDPGTEHGLF
                     SIRPHKQVYLLPVRYTTNVNNQPSSPTQGTFPQSLGYSNTEVKFQFSAKLKAIEIKER
                     FDLWIAYTQLSFFQMYNKSKSAPFRESNYEPEIMGVIHTDYDLFGLKGRFINIGLVHQ
                     SNGRQDPLSRSWNRIYAQFGFERGGFGLTIRPWYRIREDWTNDNNPDITAFVGRGDLV
                     ATYQKGGHNVSLLLRSNVDFWNFRGAVQLDWSFPLYRELKGYVQVFTGYGESLIDYNH
                     NQTTLGAGFLLIDWM"
     gene            complement(10572..11294)
                     /locus_tag="VFR82_04295"
     CDS             complement(10572..11294)
                     /locus_tag="VFR82_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEU4500849.1"
                     /translation="MHKTTIERMHPIRAYAAVMAVLVLGLIMTPVTGWADDASDAKKL
                     IDQAENTLKNFMNDPDMKWFGSHLQEAKGVYIVPKLTKGAFIFGVEGGNGVMLARDEK
                     GSGWSEPVFYETSAASFGLQAGAQSSEAVVLVMTPKGVDSLLGNKLKLGADGSVAIGP
                     KGMGADTNLMADFVTFTRAKGLYAGVSFDGASIRTRDDLNAAYFGQDVRPSDIIIAHK
                     AKPNQNSKALQAMLTKAATAKP"
     gene            complement(11319..11885)
                     /locus_tag="VFR82_04300"
     CDS             complement(11319..11885)
                     /locus_tag="VFR82_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEU4500850.1"
                     /translation="MRRRRRGCLLILAVSLVACTSFKVNTDHDPTVDFSSFKTFAFAG
                     PAELNKGGIYDNSLTRRRVESAVVRELTGKGLRQVGLDEPQDLLVHYWVNIQDKQRID
                     STGPTVGVARYPGGYGWGAGYGGGVTTYEYREGTLILDLIEPAKKQLVWRATIVGTLQ
                     DATNENVELGNKAIAEAFESYPPSAKSR"
     gene            complement(11951..12295)
                     /locus_tag="VFR82_04305"
     CDS             complement(11951..12295)
                     /locus_tag="VFR82_04305"
                     /inference="COORDINATES: protein motif:HMM:NF015069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HEU4500851.1"
                     /translation="MLNMSMPLHELIGFVAGFGTTFAAMPDLISMLKRRSCEGMNPTM
                     AAIMGTFQIVWVYYGILVESRPVIVWNTIAVGINFLVVGAYFYFACSGPRHAQVVNGS
                     HSGEGPKSSPPR"
     gene            complement(12342..>13391)
                     /locus_tag="VFR82_04310"
     CDS             complement(12342..>13391)
                     /locus_tag="VFR82_04310"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU4500852.1"
                     /translation="PSGLTVKPSESKEARQARLHLQFEQLAVVRPELILLQEVNPLPE
                     MAETYVAAMKRAGLEYSQVHQVDACGVRLAPGLAVVPHLNNGLVVLAKAPLRLRKVAG
                     LKLSGGVGRCVDYMGLQTGELRYALVAEVENPDTGKKLLAVSLHLHSGIERNAFFIQK
                     INEAVEQGRARREDFEGIVAAIEQDQSRRLDEIRLLVTEIQKLDARGHYIGTIVGGDF
                     NFEPDDPEYRELERAGLKDTYVMGNSQSEIYSLDPQQNVIAGKGVQEVPSALREAMKR
                     LPQSQQQKILEGYQKGISQARRVDFLFLMRKPSDGPTGCFHQELFGKPATVSIQPGSD
                     HYGVLVTYLADASRC"
BASE COUNT         2862 a         3926 c         3555 g         3048 t
ORIGIN      
        1 ctgcccagaa ggaattgacc tgcgcggtca cgctgaacaa agagacatcc cttctggacg
       61 gatctccgcc gactgatgct tggtatgaca taaggccaat caaaaaggat tccagccgag
      121 gaagaaaccg ggcagtggca attgccgggc cgaccgtata cttgccgaag cctaatgcat
      181 cattcgcggc ggtagggaac gtggacattg ctccgatcaa tacggcgtat tcgggagtgt
      241 tgtaggcacg ccagcctgcg gtgacagcga ggtcgctggc gccgctatga ctaggtgttc
      301 caggcctatt gggatcgctg gtgcgaagaa acggcatatc cgttcgaagc agaaagtcgc
      361 cacgaaacgg aagatcaacg cgcacagtgg ataccgtggc ctgggcacct cgaggcaaat
      421 cgaggtattg gctggtcaac tgcaatcgcc cgacaatggc ggtgggatcc gttccgtatt
      481 tcgcggcaac tttgcggagc cgctcagcct cttcccgctc ttcgggagtc atgggttgtg
      541 gaccgatccg tttgaaaacc cgacgtcttg gttctttctc ggtcggtgag acgggtcctt
      601 caggttcgtc ttctacggtc tgagtctcgt ccattcggtt cgatggggtc tcgtcgagac
      661 gatcggtggg gtccacctct ccatgagccg gtccaggacc tgtccagctc agcatcataa
      721 ggatacccag tactgccgcg ccccatgatc gaccgcatga cctctcgctc atctaccgtt
      781 agactcacgc atgatccggc cccccggcta attcatcaat ttgtcagcgg cagcgcgaag
      841 ctggagtgac ttgggattgc cgatctcctt cttcacgatg atgtcggtgg ggcgcaccgg
      901 tttcccatag taggcctggt tggaatcatt cgagaccgaa acgaaggctc cgtcgatcgc
      961 catcccggca aaggcacctt tttgcctcgc ataggagatg atgtcggccg caagaccttg
     1021 agctgacatg ccggcgccga ctgggccggt ggccatgccg gcattggctc cgagcttgaa
     1081 gtcgttcgtg taaaactcct ccagcccttt ccttgtccgc acgatcagaa ccatctcgga
     1141 gacgtctgcc ccgatctgtg cgccgaagct agcggatcca atgttataga acactggatc
     1201 accccattta ccggtcctct cgtcccgagc aatcaacacg ccgcttccgc cggctccgcc
     1261 gaagatgaac gctccccgca tgaattgtgg aacaatgaac agcgctctgg cttccttgtt
     1321 cagatctttc aacgactctt tcagttgggg atcggctgca aaggcttcca gggtcaattg
     1381 cgccttgtcc acaagctgct gttgtgcgac tgcctccgcg gaaaatgccg gagccgcacc
     1441 gaacatgact agcaccaccg ttattccggc gcaggccgac acgagtctca gtgcgccaac
     1501 cagttttgac atcatagatc atctcctctt agaaaccctt gctactctcg ggtggcgccc
     1561 tctcagaaag ggattcaaaa ttaaggtcaa tctcaccttg ttcatcgaac ggcgccttga
     1621 atgtgctggg agcgtccaca aatgccaccc ctttgagccg atagctgacg tgttgtccct
     1681 ctcgcttagt gagatctcga atctgccgga gaaagctcgc caatgtcgtg aagacttcga
     1741 cgtccactac ctgtgaacca aaccgcggta ccgccacgtg ttgccctgac attccgcttg
     1801 caaattcatg ggcgttcaat tcgatgtcaa accgcaggcc gttgatactg agctcttggt
     1861 cgttcgggtt ttgaatccgc aatttcacgt cgaatcgctg ttcgaagatc gccatgtctt
     1921 tgggagtcaa atcggtaata gtgacccgcg gcgcttcaaa atccttcggc atcgtcgagc
     1981 aggagaccgt cagcgcgagc accggtacgg ctatccacct gcatcctatg attcccttca
     2041 tgatcgtgct cctgtcgaag ttattggacg gcagtcgcca gaagatttct tgcgggttct
     2101 ccccccgctt tccccacaaa atccgacatg atctgtgtaa acttgggaac catttcggat
     2161 cccatgccca accgggagaa ggccgacgtc aaccccgcac ggtcgccaac cggtcccgta
     2221 acggcgccga ggtccctggc ggtcttcatc aaggattccg agccgggaat caatgtgctc
     2281 aacgccgtga agtccatccc cggcaatcgc tccttggcga ggctcaggat tgaccctaca
     2341 ccacccatcg cttgattcga cgtaacgcct aactggttgg tgagcagctt cgtcagggtg
     2401 tcggcagcgc ccatggattg catttgtgca caaccggagt ggaggaacgc agtcagaatc
     2461 aatagagcga accgtgatcg tctgagccac atgtggtcct cctgggttat ggatgctgcc
     2521 gcatcaccat ctcatcttgg gcgccatacg atgactcata atgtcaacac gtgaaacgcc
     2581 catggagcga gctcgacata gagtcctgcc tcgcggatct catccaagct gcgttcatac
     2641 tgtgtcccgc tgatctcatc gatcatgtgc acctctctcc catcacgcgt atctaccggc
     2701 aactgaattc taccctgaga cggagagtcg gagagattga cgactatgag atggcgtgtt
     2761 tgttcactgt gtacgtacca agtgacaaga ttcaggtagc tctggttgtc gggccatccg
     2821 gtccgctcac acagctgcca ctcacccttt ttgaaatcct cagcccgcag ggtcttgagc
     2881 agcttcctat agaagttctc caggctgcga tcactcggct cttcaggacg acgccgcaag
     2941 aagaccggaa gcctgacacg ccgcccttcg aactgcccct catggaacaa gactgcgccc
     3001 tgcagcgtcg atacggtaac ggccgccgcc tgcgcctttg aagaggagaa tgccgccgca
     3061 gcacgtggct catcgtgatt ctcgatgaat cggatcagct tttgctgata cgcagcctca
     3121 gcgcacaggt gcaggcggat gctttcagct gggccgtgct cgaggcgatc gtataaccgc
     3181 ttgtcataac aatagtcgaa cccttgccgc tgtaagttga actcgaggtc ccagtatgcc
     3241 tcggcaataa agagcacatc cgggtaccgc tgtttgaccg tccctattat gtccgtccag
     3301 tactcgcttt gaggtattcg accggcccgt gtcccccagg tccgttcgaa aatggaattc
     3361 atgagcagca tggccatatc acaacgcatt ccgtcgcact gcccggcaat tgacaggacc
     3421 gtttcgatcg ccgcttttcg caagttagga ttgaatgcat tcagttgtaa gacgtcagac
     3481 caggccggaa aatagggatc gcggccgcag gccaggatgt actctccgac ccggacaaaa
     3541 tcgtcaggtt tggtggacag gtcatcggag ttgccttgga tgaagtagtc cggatgctca
     3601 gtggcccagg gatgatcggg cgccacgtgg ttggggacat aatcaaggat caatcgcatg
     3661 ccccgttcgg ccaactgcgc tctggcatgc gccaaccctt ccggaccgcc tagtcgagca
     3721 tccacacgat aatcccgaat gcagtaggcg gacccgacat tgtcctctcc ggtgaaatca
     3781 ggcagcactc ggcgaaactc ttgcaagagt ccgtcgttcc gcatggagat cgcgacaccc
     3841 gccggacttc gttcccacac tcccatgaac cagacggcat caaatccata ggcagcaatg
     3901 gcatcccatt gcttcttggg cacgttcccc aatgtggtgg gacgccgtgc acgctcactc
     3961 agttcataga gccacaccca cgtattgatc tcgtaaatcg ttggatgacg gggccattga
     4021 gccacgggtt tttgtttcat tcaggcgtca ccgtgccgtt tgtcaccggt tctgtatagt
     4081 tcatagacgg cagcccagcc tgcataatgc cgagggggat ttccatctcg aggcagtcct
     4141 tcgttcatcg gattggaaac ggaagtcaga gcaagtactt tacaatgcgg aagtcctcaa
     4201 gaatactgtc agatttgaca gtgaaggcga cacgttgaat cccttcgttt gattttttgt
     4261 cgcctcctct acggttaacc tgcttgtaac attgcgtctc tataggacaa agtccgatgg
     4321 gcgctcttga ctgtgaatat aggacgggtt catgctgggc atcctttcaa caccactcaa
     4381 gtcgattgga ggtcagcatg ctgaccacgc ctatgtcatc ttcaacgcaa gaccagggaa
     4441 gttacgcata tccgcagaga ggaagtgctg caggccaaac tcaaccgcac taccaggatc
     4501 tcgcgcgtct gttagaagcc attgtctggc gcgcagatgc taccaccctc gaaatcacgt
     4561 atgtcagtga acaggcgaag cgcattctag gctaccccct aagccaatgg cgacaaccag
     4621 acttccagga gacgcacctg catcccgagg accgattgaa ggtactcgct tgctaccgtg
     4681 aggccattcg tgacggagaa aaccatcggc tggattaccg catgagggca gctgatggcc
     4741 gagaggtctg gtttcacgac tgcgtccatg ccatcaacgt cggggcacgc aaggaattga
     4801 tcggtgtcat ggtggacatc accgaccgaa aatctgccga ggcctgtttg actgaaatga
     4861 cagggcgctt gatccgcgcg caggaggagg agcgtagcag aattgcacga gaactgcatg
     4921 atgatttcaa tcaacgcttg gcgctactcg ctatcggtct ccagcgtctc gggcacacgt
     4981 tcgaatcgga gaccggagtt gccgctcaag tctcggatct ctaccatatg acacaagaaa
     5041 tcgcatccga cgtgcatcgc ctgtcccacc agctccaccc cgcaaaactg caacatctgg
     5101 gtctcgtccc tgcgatcaag ggattgtgtc gggaactgtc cgagcaatat ggtgctgaga
     5161 ttgactttgt ccatcgcaat gtcccaaatc cgattgccca agaatctgcg ctttgtatct
     5221 tccgtgtcgc gcaagaagcc ttgagcaata ctgtcaagca tagcggcgtg aagcaaggaa
     5281 agctcgagct gttttccaat cggggtatct tgcatctatg cgtctccgat tcaggcacgg
     5341 gctttgaccc cgaagccatt tcagccaaag gacggcttgg cttgaccagc atgcaagagc
     5401 gggtacgggc cgcggggggt accatctcag tcgaatcccg tccgtctacg ggaacgcgca
     5461 tttccgtgca tttggccgcc tagatgggag aggattccgt gcagcgtccg cgtgtcttgc
     5521 tcgccgatga ccatcgcatg cttgccgaag cctgtatcag gctcttggag ccggaatgcg
     5581 aagtcgttgg cactgtgacg gacggacggg cgctgcttcc cgtcgcgcgt gatctgcatc
     5641 ctgatgtcat cgtgctggac atcggcatgc ctttgttgaa cggactcgat gcgggcagac
     5701 aaatcaagca ctcgatgccc ggagtcaaat tgattttcat gacgatgaat gaagatcccg
     5761 atctcacgag ggaagctctt cacatcggag cgtccggata cctgcttaaa agctcagcgg
     5821 catcggagtt gcctgaagcc atccgtgcgg cagtccgtgg cgacacctac gtcgactgcc
     5881 gtgcatctgc caagttggat gattccttta ttcaagaagg cgagcgttcc tcaaccggcg
     5941 aactgactgc gcgccagcgc gaggtgcttc agttgctcgt ggaagggtta tccatgaaag
     6001 aagcggcccg tgttctccgc gtgacagcgc gcaccattgc attccataaa tatcgagtga
     6061 tgcagcagtt tcgcctcaag agtaacgcag aactcattca attcgcgatt gagcagcgtg
     6121 tcactctggg caccccgcat caatactagc cacaccgcac tgcttgaagg atcacgatca
     6181 atcttgccgg gttcaatccg gaggggacca attgaatctg tcaattttgc cagttacgcc
     6241 cgtggagggg aagtgataag aagctgcgct cgcctctctt tttccatgga gcagccatgg
     6301 ctcgatatcg gggaagcagt tctgacgggt ccccttctgt ccgcgtcctc ctagccgatg
     6361 acaacccatc gatgctgcag gccgcgcgcc gtatcctgga gcctgagttc caggtggtgg
     6421 gaaccgtcaa tgacggtgag gccgttcttg aggccgcaga cgcactgaga cctgatgtct
     6481 taatactcga tatatccatg gggatcctga atggcctgga agcggcgcgc ctgctgacac
     6541 gcactgggtc caatgccagg atcgtctttc ttaccgttca ccaggatcaa gaatttgtgg
     6601 aggaagcttt ttccgtcggg gcgcttggct atgtcgtcaa accgcgactc ggcacagatt
     6661 tgcttcccgc tgttcgggaa gcgctggtgg gaagagcctt tgtgtcacct catctcggat
     6721 cgccgaagag gtgctgatgg catgtgcgtc tgcgaactgg gttgctcccg actacgtcac
     6781 cgcagctcca cccacatcgc gccatcggcg atgatatgca catccactcc gcctgctgtg
     6841 ggatacacgc cacgaggacg aagcgtcaga acttctgctt gaagccgggc atcgggtccc
     6901 agatctcgag tcagagtcgt ttcgagacgt gatcgaagcg tctgaagctg gctggagagg
     6961 tcgatcaccg catgggcctt caggcttgac aagatgttct cgtgaaacat ccagtttgcc
     7021 gaacgaatca gcacattgcg ggtatccaga gtgaagtcca aatcgacgaa tcggaggatc
     7081 ccgaggccct cctcgaacac cggtgtgcct gtcgcgtaca ggctcccgtt gactcctcca
     7141 gagacccgta gtgcgaggat cagcttgtca ccgctcccat agagttgcac accctcgacc
     7201 ttcaatgtat cgccgacagg agtctgaaat tcctgtccaa gcatctcctc ttcaagccgt
     7261 tgattgatcc gtgcatattc gacagtgatc ggcagcgcga ggtgaaaccc ttcgaggggc
     7321 agggcagcca attgcagcgg cggcagcgac atcgaaacga tcttgggctt ggggccggtg
     7381 acaattttag gctccatcac tagattgacg gacgacttca ccaacaacgt gccattggac
     7441 gtgatcggac cgacccgtgc gtcgcgcgga ttgaactcga gccattgatt gtgggttaat
     7501 tcgattggct cctgcagttt ctgccagact gtctcggccc gcttcttcgc ttccgttcgt
     7561 ccgcggatga tccggtcgat gctttctcct aacgcggcaa gccgcggctg caataccgcc
     7621 ttcaggaaag gcgtggcctc gaggtcaaga cccgcgagct ggcagggttc aacgaaaacc
     7681 ggctcctcga aggttgtgtg agattgaacg cgccagtgat cggaccatgt gagggcggag
     7741 tgggccgtaa gattcagtcg cttcggcgga tcgtcgccat ggccgcaacg gccttcgacg
     7801 aactcttgcc cctggccctt tcgcgcccat acccgatagc gcacgtggtc aaaccacacg
     7861 tcaaaatgat tttggttcat ccgatatcgg aacgaccctc tgaccaggcg atacaagaac
     7921 cgtacgtcat cccgctctcc aagggttccc gcatcgttcc agtcttcctc tcgggacgca
     7981 accggaggaa acccctcatc aacggcgaca gccaattctt ccgccgggag ccggacttcg
     8041 agattcacga cggacgcctc aagggcaggc ggcggaggcg caggcttgag cggatcgttt
     8101 ggcttcggaa tgttaagtgg cgagcctgcc catgcgctga tcgcatagac atgcgctagc
     8161 aggattgaac agcacaatgt gccgaaccat ggttgtctcg ggcaacttcg tggaggcgtc
     8221 aattcgctcc ctgcgatgga gaaatcacat gccggtcaac tgacagtgta gccccgtccg
     8281 gtcatgcagg cgccaaatgc gcgcttgtag ccgccaacat ccgcgttata cgcctgcttt
     8341 gctttttcct gggccgcggc ttgctgctcc gctgcgtgcc ggcggcgcat cgcacctcca
     8401 gccgcgccaa ctcccgctcc aatggccgct ccctttcctg catcgccgcc gatggcaccc
     8461 ccgatagcgc cgattgcggc gcctctcgcc gcgcccttca cggcgccgcc ctgcggcggc
     8521 ggagcagcgg cttgctgcat cggctggttc ggatcgaatc cggtttgctc tttggcccat
     8581 ttgtagcaac taaactcgtc ctgttcctgt tgatccttcg tttggccctt actgggatac
     8641 acgaacacgt ccgcagctgc aagcgcttca ctcgtgatga acagcagccc catcacgatg
     8701 acaatcaatc gtgagtaatc catacatgcc tcctctatct cacgaatcgt acacacagtc
     8761 atttcgtcag ctccatggag ggatggtccg tgcccgaagc agaagtgtca cgatcccctg
     8821 tagaccctgc ctcgcctgcg gaagatcccg aagcggccgc gtgaggaaca tccccgatta
     8881 gcggcagatt gcccagaatc gcgaggcgtg tgaccattcc gacgatatcg tccaactgca
     8941 tgaagcgagc ggcagctacc ggattcagtt tcttgctgta aatgtggaaa tacttctttc
     9001 tcagtgcaag gtccgcctgc tggttctcgc tcgactgctt gacgatcttc atcacatcgt
     9061 cgttggtaag cgatgaatat ttctcaatat aggttcccag taagcgaagt cgtttcgcct
     9121 caagttggtc cttttccttt tcataccggt gatacacgcc ccaaaatacc tcactttgct
     9181 gctcatcgag gcccatattc cgctcgaaca cctctttgcg ctcggtcgac agaatatact
     9241 gcagtttgtc ggggtccaaa tttgggcgaa tgcgcacaac cttcagtgtt gattccggca
     9301 atgttgattc ctgtgcatgc agactaggaa ccatcaacac acccatcaga cacatgccga
     9361 ccaccacatt cggtttcatg acggctcctt cctcgtcaca gcagatggat gcaccttttc
     9421 gccgacctca cgctatcatc atttgacagg gacagacact gtcaaaaatg acagagcctt
     9481 ggagatctca catccagtcg attagcagaa atcccgctcc aagcgtcgtc tgattgtgat
     9541 tgtaatcaat caagctttcg ccgtaaccgg tgaacacttg tacatagccc ttgagctctc
     9601 ggtacagtgg aaaactccag tccaactgaa ccgcgccgcg aaaattccaa aagtccacat
     9661 tacttcgcag aagaagtgag acgttgtgtc cgcccttttg ataagtcgcg acgaggtctc
     9721 cacgtccgac gaaggcggta atgtcggggt tattgtcatt ggtccagtct tctcggatgc
     9781 ggtaccacgg tctgatagtc agtccgaacc ctccacgctc gaatccgaat tgtgcgtaaa
     9841 ttcgattcca gcttcgcgac aaagggtcct gacgtccgtt tgactgatgg acaaggccta
     9901 tattgatgaa ccgtcccttc agtccgaaca gatcgtagtc agtatgaatc acgcccataa
     9961 tttccggctc gtaattgctt tcgcgaaatg gcgccgattt cgatttgttg tacatctgga
    10021 agaaagagag ttgcgtatag gcaatccaca aatcgaatcg ttctttgatc tcgatcgctt
    10081 tgagcttcgc agagaattgg aacttgacct ctgtattgct ataccccaac gactgaggaa
    10141 acgtgccttg ggtaggactc gacggttggt tgttcacatt ggtggtatag cgaacgggca
    10201 ggagatagac ttgcttgtgg ggacggatcg aaaacagccc atgctctgtg ccagggtcca
    10261 gttcccagcg cgtccccatc aacgaagctt tgcgagcgga gctcgccaaa ccagcgtcgg
    10321 acggttctgg tgattgactt tgttgccggg ccgccgcgtc atcgtaacat ttcaagcgtt
    10381 cggcgtccgg ccccaggtcg gagcagacag gtacggtccc cggcgcaccg gggggcggcg
    10441 aatctgcatg ggcggcaatc tctcctgcaa gaggaaaact gctgagctga acgatgcaga
    10501 gtcctgccgc ccaccacggc gtcctcgggt gtctcacgtg ttgatgtcgt gttgcttcct
    10561 ttgcctctcc gctacggttt tgcagtagcc gccttcgtca gcatggcctg caacgccttg
    10621 gaattctggt tgggcttggc cttatgcgca ataataatat ccgacggacg gacgtcctgt
    10681 ccgaaatatg ccgcattgag gtcgtccctt gtcctgatag acgctccgtc gaacgagact
    10741 ccggcgtata atcccttcgc gcgcgtgaac gtcacaaaat cggccatcag gtttgtgtcg
    10801 gcccccatcc ccttcgggcc tatggcgaca gatccgtcgg cgccaagctt gagcttattc
    10861 cccaacagcg aatccactcc tttaggcgtc atgaccaaca cgactgcttc ggaggactgg
    10921 gccccggctt gaaggccgaa actggctgcc gaggtttcgt aaaacactgg ctcgctccat
    10981 ccactgcctt tttcatcacg cgcaagcatg acgccgttgc cgccttctac gccgaagatg
    11041 aaggcaccct tcgtgagttt tggaacgata tagacaccct tagcctcttg caggtgactg
    11101 ccaaaccact tcatatccgg gtcgttcatg aagttcttga gtgtgttttc ggcctgatcg
    11161 atcaacttct tggcatcgct ggcgtcatcg gcccacccgg tgaccggcgt catgatcagc
    11221 cctaggacca acaccgccat gacggcggcg tatgcgcgaa tggggtgcat cctctcgatc
    11281 gtcgtcttgt gcatagttcc ctccttgttg gaaaattctc agcgtgattt cgcggatggc
    11341 ggataactct caaacgcctc ggcaatcgcc ttattgccga gctcgacatt ctcattggtc
    11401 gcgtcttgca atgtccccac gatcgtggct ctccagacga gctgcttttt cgctggttcg
    11461 atcagatcca gaatcaaggt gccttcccta tactcatagg tcgtcacccc tccgccgtag
    11521 ccggcgcccc atccataccc cccagggtaa cgagcaacgc ccaccgtggg gccggtgctg
    11581 tcaatccgtt gcttgtcctg aatattgacc caatagtgaa cgagaagatc ctgtggctca
    11641 tcgagtccta cttggcggag tcctttcccc gtgagctcac gcacaaccgc agactcgact
    11701 cttctacgcg tcaacgaatt gtcgtagatt ccgcctttat tcaactcggc cggcccggcg
    11761 aaggcaaagg tcttgaacga cgagaaatcc accgtcggat cgtgatcggt gttcactttg
    11821 aatgaggtac aggccactag gctgacagcc aagatgagca ggcagcctct tcgtcgacga
    11881 cgcatgaggt tcctccactt caatgttgta ggccacgctt ccagatgcgc ctcctgttgc
    11941 tgagctccac ctatcgaggc ggagagctct tcggcccttc gcccgagtgt gaaccgttga
    12001 caacctgggc atgccgtggg ccgctgcagg caaaatagaa gtaggcaccg accaccagaa
    12061 aattgatccc cactgcgatc gtattccaca caatgactgg cctcgattcg accaagatcc
    12121 cgtaatagac ccacacgatt tgaaaggtgc ccataatcgc cgccatggtc ggattcatac
    12181 cttcacagga acgccgcttg agcatagaga tgagatcggg catggcggca aatgtggtcc
    12241 cgaagcccgc gacaaagccg ataagttcat gcaacggcat tgacatgttc aacatggcgc
    12301 accttcaatc tgttcagatt gcaagcatga ggcctgcccg gtcaacatcg ggacgcgtca
    12361 gcgagatagg tcacgagtac accatagtga tccgatccgg gttgaattga aactgttgcc
    12421 ggtttgccga ataattcctg atgaaagcaa cctgtgggac cgtccgaggg tttcctcatg
    12481 agaaagagaa aatcaactcg cctcgcctga ctgataccct tttgatagcc ctcaagaatt
    12541 ttctgttgct ggctctgagg caaccgcttc atcgcctcgc gaagcgcaga gggcacctct
    12601 tgtactccct tccctgcaat cacgttctgc tgagggtcca gactgtatat ttcgctctgg
    12661 gaattgccca tcacgtaggt atcctttagc ccagcccgtt caagctctcg atattcagga
    12721 tcgtccggtt caaaattgaa atctccaccg acgatggttc cgatgtagtg gccccttgca
    12781 tcaagtttct gtatctctgt aacgagcagg cgaatctcat caaggcgccg gctctggtct
    12841 tgttcgatcg cggcaacgat gccctcgaag tcctcccgcc gtgcccttcc ctgctcaaca
    12901 gcctcattga tcttctgaat aaaaaaagcg ttgcgttcaa taccagaatg gagatgaaga
    12961 ctcaccgcca gcagcttctt tcctgtatct gggttttcga cttcggcgac caaggcatag
    13021 cgaagttcgc cggtctgcag acccatgtaa tcgacgcagc gtccgacccc tccgcttagc
    13081 ttgagccccg caacttttct taatctcaaa ggcgctttgg caaggaccac taacccattg
    13141 ttcagatggg gaaccaccgc gagccctggg gcaaggcgta ccccgcaggc atcgacttga
    13201 tgcacttggc tatactccaa cccagcgcgt ttcatcgcgg cgacatacgt ctcggccatt
    13261 tcaggcaaag gattgacctc ctgcaaaagg atgagctcag gtcggaccac ggctaattgt
    13321 tcaaattgca gatgaagcct tgcctggcgt gcctccttag attcgctcgg tttaaccgtt
    13381 aggccgctgg g
//
LOCUS       DASTQY010000134         9563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_343583, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000134 DASTQY010000000
VERSION     DASTQY010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9563
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343583"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VFR82_04315"
     CDS             <1..839
                     /locus_tag="VFR82_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4500853.1"
                     /translation="RRLEQLKAEGVEFKTGVTVGTDMTGEQLRAQFDAVGLTMGAEQA
                     RELPIPGRELKGVHLAMEYLTQQNKRTAGIPISDEPITAKGKRVVIIGGGDTGSDCLG
                     TTHRQGCVEVHQFELLPEPPPTRAESTPWPLWPMQLRTSHAHEEGCDRQWSVATTKFT
                     GHNGHLTKLHAQRVKFEGGKFTPIPDTEFAMDVDLVLLAMGFTGPVKNGLLDHLGVSY
                     DSRGAVAVDDNFMTNLDGVFAGGDTKRGASLIVWAIAEGRKMAAGIDKYLQAGRSAKL
                     RC"
     gene            1264..1569
                     /locus_tag="VFR82_04320"
     CDS             1264..1569
                     /locus_tag="VFR82_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500854.1"
                     /translation="MHMLMVVFRASLKERVHELLDRCHVKAFTEVNETVGYGQTGPAE
                     GLAFYPGTNSVIFVALDPDHAARVANAVKQWYAEAAKHPGWEKPALRVFSWPAEQIV"
     gene            1763..3478
                     /locus_tag="VFR82_04325"
     CDS             1763..3478
                     /locus_tag="VFR82_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HEU4500855.1"
                     /translation="MATNLNLLLLEDSPADAELMLEALRESGYDPIFRRVDSKDAYLR
                     ELDQPPDFILSDFSMPQFTAQDALKLMKERGLDLPFIIVSGCMGEDMAVECMKAGASD
                     YLLKDRLARLGHAVTQALERKRLIEEKRQAEQRLFLETFHDSLTGLPNRALFMDRLDR
                     VFLQSRRQPAHLFALLHLNLDGLTMVHSGMGVSSADRLLIEVSQRILRRIRSVDTLAR
                     MEGDAFTLLIDNLKAMGNATRVADRLQQEFATPFTLEDREVYLTPYIGIACSHTGYEN
                     GEPLVRDATTAMLQAKATGRAGFVIFDKAMHQQAMARLKLEGDLRQALDRKEFRLAYQ
                     PIVQLQEGRITGFESLVRWQHPEYGLTRPDVFLDIATELGLMKGIGEWCLGEGCRQLK
                     LWHDQFPRIRPLTISINFSIQQFAQDRLAVLIKRVLDKSGIDAASVKIEITESEMMQN
                     PEAVNRVLAEIKEQHVDTCLDDFGTGYSSLSHLQQLPIQFLKIDQSFVRRLGTDDDAL
                     AIVKTIIALAHQLGRQVIAEGVETAEQLAILRSLGCEYGQGYFFSKPLSPEDAGTLLA
                     SARRW"
     gene            3544..4284
                     /locus_tag="VFR82_04330"
     CDS             3544..4284
                     /locus_tag="VFR82_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU4500856.1"
                     /translation="MASVQRFSAKVIAIRHLTHDVRQIDFRLLEPPEIRFKAGQFVSF
                     EVPDARTGRTVTRPYSIASAPSTPDTVSLLLNFVPDGPGSTYLFKLNEGDTTTFSGPA
                     GNFYLRDDPERELLFVATGTGIAPIRSMLLANAERPTPRTTTLFWGLRSQRDLYHEDN
                     FESLVRPVPGSSVVITVSRPEAGWTGVAGRVTSLVEKNVQDVKRLAVYLCGNSAMIKD
                     VTALIQAKGLCPIYREKYYDDAGESDRD"
     gene            4490..4804
                     /locus_tag="VFR82_04335"
     CDS             4490..4804
                     /locus_tag="VFR82_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500857.1"
                     /translation="MTKIRSVLTVAAVVASLATAFAQTPGPEASPAPTPAPNPEITMP
                     PPAPSSAIGEEKKPEKPMKGKHGKAKAHGKSSEKKRGLDRADEAAGEHGKQGRGQARA
                     QR"
     gene            complement(4885..5313)
                     /locus_tag="VFR82_04340"
     CDS             complement(4885..5313)
                     /locus_tag="VFR82_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500858.1"
                     /translation="MARTLESSTEAGAGLRRLLSEVRRGLLGLHKALIVAEQLTYERI
                     NGRVGSTGQLLQLVLNDPWFTWLHPLSQLVIRVDELLEEGEELTTVEVEHFLADVRAL
                     IRPSEEGDGFERSYYEALQREPDVIFAHVEVKKLVTKAAA"
     gene            complement(5387..5755)
                     /locus_tag="VFR82_04345"
     CDS             complement(5387..5755)
                     /locus_tag="VFR82_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500859.1"
                     /translation="MGLMKQVRRSVIAGALMGVWLAQPHWVLGEVLSDHRVTAGRDNT
                     IGVGTGGGTDSLSAREFRLAPSLSERNAPEQTTTPLGVPSPPHQLTPAPVLPFHPNRH
                     LIPSPTVPSNGPMGGGHLGR"
     gene            complement(5794..6225)
                     /locus_tag="VFR82_04350"
     CDS             complement(5794..6225)
                     /locus_tag="VFR82_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500860.1"
                     /translation="MEASSHTTALVPVDPRRRTVLSIRESLIGLHGALILAEQQTYER
                     VSGPVQSMEVLIELLQKDPWFTWLHPIADLLVRIDHLLQDDTCDITGDNVAHLLGEID
                     SLLHPSVEGEGFERAYYEALERAPDVVLAHFRIRKLLTNAA"
     gene            6347..6676
                     /locus_tag="VFR82_04355"
     CDS             6347..6676
                     /locus_tag="VFR82_04355"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4500861.1"
                     /translation="MERRKHRRVPIQIQGLLLGNSHEVEGHTLDLSLGGARFASDVDV
                     FPGKTIVVRLVIPGLEEPLSIPEATVQWVDEQTFGVEFQEIPPDELDDLEELINEFDE
                     TENSGHA"
     gene            complement(6714..6878)
                     /locus_tag="VFR82_04360"
     CDS             complement(6714..6878)
                     /locus_tag="VFR82_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500862.1"
                     /translation="MAKTATYCSECYNKVGRVEDAVVKAAEASGKTPMTGQGTCCKCN
                     KATIVVYYEG"
     gene            complement(6972..7661)
                     /locus_tag="VFR82_04365"
     CDS             complement(6972..7661)
                     /locus_tag="VFR82_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500863.1"
                     /translation="MNAASISLTRMVLIGFLVAIVTLVGCATSTGDRSRRILTGQTCC
                     KSAEATSRRAAIVQTAVRLVGATTIHTKGRRIAYDCAGVTRAIFLEHGIDLYDSAAPD
                     PKANGVKLIHHHVSRQGKLHQGPAVRPGDLVFFDNTWDYNGDGMTNDPLTHVGIVERQ
                     EADGTIVFISRVAGAIERYRMNLALPHLHRTTEGRLLNDYLRRKDFDDPATTAYLTGE
                     LFAGFGSPTGL"
     gene            complement(7768..8700)
                     /locus_tag="VFR82_04370"
     CDS             complement(7768..8700)
                     /locus_tag="VFR82_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU4500864.1"
                     /translation="MCRVLVCLAASVLSLISISPSQAASAHKPSPQLAGHLASIAGLA
                     QSAPTVQIPAGEFTVGSKRIDDDPYGLWTQFDDTELPQQRVWLDAYAIDRHEVSLAEY
                     LSHLRQGNQHPADELQKLIWHVITVHSVSDRTLARWPALYMTWQEAARFCISKGKRLP
                     TEAEWEKAARGPDGQLFPWGNAAPSPERAMFGQHHVHEIPILAAVDSHPEGRSPYGLH
                     HMAGNVAEWVQDWFGLDYYAYMPRENPSGPASGRYKGVRGGSWKSKIIMLRTATRSGF
                     SPDQRSATIGFRCAQSSANPGPPHGNDSPHAAGK"
     gene            complement(8700..9428)
                     /locus_tag="VFR82_04375"
     CDS             complement(8700..9428)
                     /locus_tag="VFR82_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500865.1"
                     /translation="MWCCRYRSILLTLVLTNFLPLSGCKPDESPFRAENDILKKQLAR
                     QESLVTSLQDGNKVMQQQIDLLNQEIRDAKKAAESARAEAKGAAEQLDKQLIQARKLT
                     ADIKKTAIEQAAQSIQLEQKGAQFEDLPRPLNAVTKTVEEALARNGYQVKVSIKADQK
                     AVIVTDRKVSNPVSLEVAGFRNQYVIAVQAQPLNVTRLSVKAEFEKLAQGGRILSVSA
                     EETAEIERRLIGEVSKALSSPSKT"
BASE COUNT         2013 a         2651 c         2850 g         2049 t
ORIGIN      
        1 accgccgcct ggagcagcta aaggcggaag gcgtggaatt caaaacgggc gtcaccgtcg
       61 gcacagacat gacgggcgaa caattgcgtg cacagttcga tgcggtgggt ctcacgatgg
      121 gagcggagca ggcgcgtgaa ctgccgattc ctggaaggga actaaaaggc gtgcatttgg
      181 cgatggagta tctcacgcag caaaacaagc gcaccgcagg tattcccatc agcgacgaac
      241 cgatcacggc caagggcaag cgggtcgtca ttattggcgg gggagacacg ggatctgact
      301 gtctggggac cacgcaccgt caaggctgcg tggaagtgca ccagttcgag ttactgcccg
      361 aaccgcctcc gacacgtgcc gagtcgacgc cctggccgct atggccgatg cagttgcgga
      421 cttctcatgc ccatgaggaa gggtgcgacc gtcagtggag cgtcgcgacg accaagttca
      481 ccggccacaa tggccatctc acgaagcttc atgcgcaacg cgtgaagttc gaaggcggca
      541 agtttacacc cattccggac accgaatttg cgatggacgt ggatctggtc ctgctggcga
      601 tggggttcac tgggcctgtt aagaacggcc ttctcgacca tctcggagtg agctacgact
      661 cccgcggcgc ggttgccgtt gacgacaact tcatgacgaa cctcgacggg gtctttgccg
      721 gcggcgacac gaagcgcggc gcttccctga tcgtgtgggc gatcgccgaa ggccgaaaga
      781 tggcggcggg aatcgataaa tacctgcaag ccggtagatc cgcgaagtta cgttgttgaa
      841 cgcacggcca acgctgtctg cgcatctcct ccttccctcc gtcacgtgcc gtcagtcgaa
      901 aacgtacaga ggctgagctc gttgcccccc tgattctttc gcactcttcg cgaatcgggc
      961 gattgcacac gtcgcaaggc tgtgatgtgg taccgatctg gcatgacttt gtggaaggtg
     1021 ctggaactcc aggcgttacc gcgcggcctt gtgtatcgcg gacggtgtgc gcgatccacg
     1081 tgtggaccag gtgacggcgg ccttgctatg ttcacgcgtt gagaaggaac tgataaggaa
     1141 ctgatagtgg aacgagcggt tgtctcctgg ggttcatggg atcaggcgcc tcgggagcgc
     1201 tttcttgaac tggacgaccg gttatgagtc tgaaccacca atgacatatg aaggagactt
     1261 cacatgcata tgctcatggt cgtgttccgt gcgtcactga aagaacgtgt gcatgaattg
     1321 ctcgatcgtt gtcacgtgaa agcgtttact gaggtcaatg aaacggttgg ttacggtcag
     1381 accggccccg ccgaagggtt ggccttttac cccggcacga attcagtcat cttcgtcgca
     1441 ttggatcccg atcatgcggc gcgagtcgcc aacgccgtca aacaatggta tgcggaggcg
     1501 gcaaaacatc ccggctggga gaagccggcc ctgcgcgtct tctcctggcc ggcagagcag
     1561 atcgtgtaac gcgccgggca gttcggaatc ctgtccatca gtgtcctccg cgtcttacaa
     1621 gggttcattc ttagggccgg ataagttcct aacatttctt ggtcgtttgc tcatctcact
     1681 ctatactggc ctacatacgt aggtgtgcat agaaggaagg acggcttccg gagaagcggt
     1741 ccgacctcga tcgaggaccc acatggcaac caacctcaat ctcctcctgc tggaagattc
     1801 accagccgat gcggagttga tgcttgaagc actgcgagag tcggggtatg accccatctt
     1861 tcgccgcgtc gattccaagg acgcctatct ccgagaactc gatcagccgc cggatttcat
     1921 cctgtccgat ttttccatgc cccagtttac ggcgcaggac gctctcaagt taatgaagga
     1981 gcgagggttg gatcttccat tcatcatcgt ctccggctgc atgggcgagg atatggcggt
     2041 ggagtgtatg aaagccggcg cgtccgacta cctcttgaaa gaccggcttg caagactcgg
     2101 gcatgcggtt acgcaggcgc tggaacgaaa gcggttgatc gaagaaaaac gacaagctga
     2161 acagcggttg tttctcgaaa cgttccacga ttcgttgacc ggccttccga atcgggcctt
     2221 attcatggat cgtttggatc gtgtgtttct acagagccgc cgccaacctg cccatctatt
     2281 cgccctcctg cacttgaacc tagacggctt gacaatggtg cacagcggca tgggagtttc
     2341 gtctgccgac cggctcctga tcgaagtcag ccagcgaatt cttcgcagga tccggtccgt
     2401 tgacacgctc gctcggatgg agggagatgc ctttacgctc ctgatcgaca atctcaaggc
     2461 gatgggcaat gcgacgcgtg tggccgaccg tctacaacag gagtttgcca ccccgttcac
     2521 gttggaggac cgggaagtat acctgacgcc ttacattggg attgcctgta gccatacagg
     2581 atatgaaaac ggggagccgt tggtgcgtga tgcgacgaca gccatgctcc aggccaaggc
     2641 gaccggccgt gcgggattcg tcatcttcga caaagcgatg caccagcagg ccatggccag
     2701 actgaagctg gaaggcgatc tgaggcaggc gctggaccgc aaggagtttc gattggccta
     2761 ccagccgatc gttcagttgc aagaagggcg gataacgggg ttcgaatcgt tggttcgatg
     2821 gcaacatcca gagtatggtt taacgaggcc ggatgtgttt ttggatatcg cgaccgagct
     2881 gggcctgatg aagggtattg gcgaatggtg cctgggcgaa ggatgtcgcc agctgaaact
     2941 ctggcacgat caatttcccc ggattcgccc gctcactatc agtatcaatt tctccatcca
     3001 gcaatttgct caggaccgcc tggcggtgct gattaaacgc gtcctcgaca agtcgggcat
     3061 cgatgccgcg agcgtcaaga tcgagatcac cgaatcggaa atgatgcaga accccgaggc
     3121 cgtcaaccgc gtcctcgcag agatcaagga acagcatgtc gacacctgct tggacgattt
     3181 cggaactgga tattcctcgc tgagccacct ccagcagtta cccatccagt ttctgaagat
     3241 cgatcagtcg ttcgtccgtc gattgggcac cgacgacgac gcattggcga ttgtaaagac
     3301 catcattgcc ctggcccacc aactcggacg gcaggtgatc gccgagggcg tcgaaaccgc
     3361 cgagcaactt gccatcctgc gttcgctggg ctgcgaatac ggtcaaggct atttcttctc
     3421 gaaacctctt tcccccgaag atgcaggcac cctgctggcc tctgccaggc ggtggtgaga
     3481 cgccgcactg tccttttcgg caagctccct tgataagatg gggtattcga agggagtggc
     3541 gtcatggctt ccgtgcagcg tttttcggcg aaggttattg ccatccggca cctgacccat
     3601 gacgtccgtc agatcgactt ccggttgttg gagccgccgg agatccggtt caaagcgggc
     3661 cagttcgttt cgttcgaggt gcccgatgcc cgaacaggtc ggacggtgac ccgaccgtat
     3721 tccatcgcct ctgcaccgag cactcccgat acggtgtcct tgctgctcaa tttcgttccg
     3781 gacgggccag ggtccacata tctctttaaa ttgaacgaag gcgatacaac aaccttctcc
     3841 ggaccggccg gcaattttta cctgcgggac gatcccgagc gagagctgtt gttcgtcgcc
     3901 accggcacag gaatcgcacc catacgctcg atgctcctgg cgaacgccga gcggcctacc
     3961 ccaagaacga cgacgttatt ttggggcttg agaagtcaac gagatctcta ccatgaggat
     4021 aatttcgaaa gccttgtcag acctgtgcca ggaagctcgg tggtcatcac cgtgtcgcgg
     4081 ccagaagccg gctggaccgg agttgcaggc agagtgacga gtctcgtcga gaagaatgta
     4141 caggatgtga agagattggc tgtctaccta tgcggcaaca gcgccatgat caaagacgtg
     4201 accgcgttga tccaagcaaa gggattatgc ccgatatacc gcgagaaata ttatgatgat
     4261 gcgggagaaa gcgaccggga ctgattgctg gctcttggca atgcaccttg tcgcggttgg
     4321 tcgaaattgt accgccgcat agccaacggg ctgcatacac ttgtgttcat gagaaaagtc
     4381 cgctcgtcta ctagggtgtt taagcatgct ggaagcggca cgaagcttgc tcagagtgta
     4441 gcgccgatcg tgggaataga tcccgtaggc ataccatgag gagaataaaa tgacgaagat
     4501 cagatcggtg ctgaccgttg ccgcagtcgt tgcgtcgttg gccaccgcat tcgctcaaac
     4561 cccgggtccg gaggctagcc ctgcgccaac gcctgctcca aatccggaga tcaccatgcc
     4621 gcctccagct ccgtcttcag ctattggtga ggaaaagaag cctgagaagc cgatgaaggg
     4681 aaaacacggc aaagcgaaag cgcacgggaa gagcagcgaa aagaagcgcg gactcgatcg
     4741 cgcggatgaa gcagcgggtg agcatggcaa gcagggccgc ggtcaggccc gggcccaacg
     4801 gtagtacgca attgtcagca agaaaaaggc ggccggttcg attgaaccag ccgccttcat
     4861 acgggtcaga cttccaaaaa ccgctcatgc cgcggctttg gtcaccaatt ttttcacttc
     4921 aacgtgtgcg aagatgacat ccggttcgcg ttgcagcgcc tcgtagtagc tgcgctcgaa
     4981 cccgtcgccc tcttccgatg gacggattaa agcgcggacg tcggcgagaa aatgttctac
     5041 ttcaacggtc gtcagttcct ctccttcctc caataattca tccacgcgaa tgaccagctg
     5101 cgatagagga tggagccatg taaaccatgg gtcgttcaac accagttgca aaagttgtcc
     5161 cgtcgaccct acgcggccgt tgatgcgctc ataagtcaac tgctcggcca cgatgagcgc
     5221 tttgtgcaag cctagcagtc cgcggcgaac ctccgacagc aatcgtcgca ggcccgcgcc
     5281 ggcttccgtt gaggattcga gtgttcgtgc cataggttta ttgtatcagg tcgcagaaaa
     5341 aatgtgtctg tccttcgacc acacattgaa cgacaatcgt ccgccgtcag cggccaaggt
     5401 gtccaccacc catcggccca tttgagggga ctgttgggga aggaatgagg tggcggttag
     5461 gatgaaaggg aagcaccgga gccggggtga gttggtgagg cggcgacggt actccgagcg
     5521 gcgtcgttgt ttgttcaggt gcattccgct cagaaaggct aggcgcaaga cgaaactccc
     5581 gggcagaaag ggagtccgtg ccgccgcccg tcccgacgcc tatggtgttg tcacggccgg
     5641 ccgtgacccg atggtcgctc aggacttcgc cgaggaccca atgaggctgt gcgagccaga
     5701 ctcccatcag cgccccggcg atcacgctcc tccgaacctg cttcatcaag cccatggctg
     5761 ctcctcactc gcatcataac agagcttccg gcgctatgca gcattggtca acaacttcct
     5821 tatgcggaag tgagcgagca cgacatcggg agcccgctcg agcgcttcat agtaggctcg
     5881 ttcgaatcct tctccttcga cggatggatg aagcaagctg tcgatttctc ccaagagatg
     5941 cgcgacattg tcgccggtga tgtcacacgt atcgtcttgc agcaggtgat caatgcggac
     6001 aagcagatcg gcgatcgggt gcagccaggt gaaccagggg tccttttgga gcagttcgat
     6061 caacacttcc atggattgca cgggcccact cacgcgttca taggtttgct gctcggcgag
     6121 gatgagggca ccgtgaagcc cgatgaggct ttcccgtatc gacaggacag tcctgcgccg
     6181 tggatccacg ggaaccagcg ccgtcgtgtg agatgacgct tccatacgtc aagtgtagca
     6241 ggcctgtacg aaacggccag cggcctcgat gcgtgcacga agacggatgc atttctgttc
     6301 caaagcgagg tattcttctc caaccgtctt cgaggggtgc ctaaagatgg aacgacgaaa
     6361 gcaccggcgt gttccgattc agattcaggg actgttgttg gggaattctc atgaagtcga
     6421 aggacacacg ctcgacctct cgctcggagg agcgagattt gcgagtgatg tcgacgtctt
     6481 cccggggaag acgatcgtgg ttcgtctcgt gattccggga ttggaggaac cgttgtcgat
     6541 tccggaggcg acggtgcaat gggtcgacga gcagacgttt ggcgtcgagt ttcaagaaat
     6601 tccgcccgat gaactcgatg acctagaaga actcatcaac gaattcgatg agacggaaaa
     6661 tagtggccat gcctagcacg ccatgctcga aggcaggtcg agcggcgtga gggttacccc
     6721 tcgtaataca ccacgatcgt agccttgttg cacttgcagc aggtgccttg cccggtcatg
     6781 ggcgttttcc cggaggcttc tgccgctttg accactgcgt cttcgacccg gccgaccttg
     6841 ttgtaacact cgctgcaata ggtcgctgtc ttcgccatag ctcctcctga gtataggtct
     6901 acacgatgcc caataagaga agtatagcgc aactacgcaa aagtgcttgg tcggcctatc
     6961 ggcctgcagg gttatagacc ggtcgggctt ccgaagccgg caaacagctc gccagtcagg
     7021 taggcggtcg tggcaggatc gtcgaagtct ttccgacgga gatagtcgtt aaggaggcgg
     7081 ccttctgtcg tacgatggag gtgaggcagg gccaaattca tgcggtaccg ctcgatagcg
     7141 ccggcgaccc gactgatgaa aactatcgtg ccgtcggctt cctggcgttc cacgatgccg
     7201 acatgagtca aagggtcgtt ggtcatgccg tccccattgt aatcccatgt gttgtcaaaa
     7261 aagacgaggt cgccgggccg gacggccgga ccttgatgaa gcttgccctg ccggctgaca
     7321 tgatgatgga tcagcttcac gccgttggct tttggatcgg gcgcggcgct gtcgtatagg
     7381 tcgatgccgt gctcgagaaa aatcgctcgg gtcacgccgg cgcagtcgta ggcaatgcgt
     7441 cggcccttgg tgtgaatcgt ggtcgcaccg acaagtctga cggccgtttg gacgatagcg
     7501 gcgcgccggg atgtggcctc ggcggactta cagcaggtct gaccggtgag aattcgacgg
     7561 gagcggtcac cggttgacgt ggcgcacccg acaagcgtca cgatggcgac gagaaatcca
     7621 atcaagacca ttcgggtcaa ggagatcgag gccgcgttca ttcttgccaa cgtagcatga
     7681 gagccacaat cggccaagta atcggccagt ttaggaaggc cggaaaccca gaagtacctt
     7741 ttgcgatttc gtcaaaaaca ttatatctca cttgccggca gcgtgagggg aatcgttgcc
     7801 atgggggggg ccggggttgg cagaagattg tgcgcagcga aaaccaatag tggccgaccg
     7861 ctggtcggga ctgaatccgc tgcgtgtggc ggtgcgaagc ataatgattt tgctcttcca
     7921 cgagccgccg cgcacgccct tatagcggcc gcttgccgga ccgctcggat tctctctcgg
     7981 catataggcg tagtagtcca atccaaacca atcctgtacc cactccgcca cattgccggc
     8041 catatggtgc aggccgtagg gacttcgtcc ctctgggtgt gagtccacag cagccaaaat
     8101 gggaatttca tgcacatgat gctggccaaa catcgcccgt tcagggcttg gagcggcatt
     8161 gccccagggg aacagttggc cgtccggacc gcgggcggct ttctcccatt ccgcttccgt
     8221 cggcagccgc ttcccctttg atatgcagaa tctcgccgcc tcttgccagg tcatgtagag
     8281 ggccggccat cgcgccagcg tgcgatccga gactgagtgc acggtgatga catgccaaat
     8341 cagcttttgc aattcgtcgg cgggatgctg gttgccctgt cggagatgcg aaagatattc
     8401 ggcaaggctg acttcatgac ggtcaatcgc gtacgcgtcc aaccagacgc gctgttgtgg
     8461 aagttccgta tcatcgaact gcgtccatag gccatacgga tcgtcgtcga ttcgcttgct
     8521 gccaaccgtg aattcaccgg ccgggatctg gacggtcggc gcggactgcg cgagcccggc
     8581 aatcgaggcg agatgaccgg ccagctgcgg agaaggcttg tgcgcgctgg cggcttggct
     8641 tgggctgatg gagatgagcg acaggacaga tgccgcaagg cacacgagaa ctctgcacat
     8701 caggtcttgc tgggagagga gagggccttg ctgacttcac cgataagtcg tcgttcgatt
     8761 tctgccgttt cttccgcact gacgctcaag atccgcccgc cctgcgccag cttttcgaat
     8821 tcggccttga cgctcaaacg ggtgacgttc aacggctgtg cttggacggc gatcacatat
     8881 tggtttcgaa agccggccac ttcgagagaa accggattgg agaccttgcg gtccgtgacg
     8941 atcacggcct tctgatcggc tttgatactg actttcacct ggtagccgtt gcgcgccagc
     9001 gcttcctcga cggtcttggt gacggcattc aacggccgcg ggagatcttc aaattgcgcg
     9061 cccttttgtt ctagctgaat actttgggcg gcctgctcga tagccgtctt cttgatgtcc
     9121 gccgtcagtt tgcgggcctg gatcagctgt ttgtcgagct gttcggctgc gcctttggcc
     9181 tcagctctgg cgctttctgc tgccttctta gcgtcacgga tctcctgatt caggaggtcg
     9241 atctgttgct gcatgacctt gttcccgtct tgcaacgagg taaccaagga ctcctgtcgg
     9301 gcgagttgct ttttgagtat gtcgttctca gccctaaaag gagattcatc cggtttacaa
     9361 ccggacaaag gcaagaaatt cgtcaggaca agggtgagga gaatcgagcg atatcgacaa
     9421 caccacatgg atggacggat tatccatgct tcggggtctc ttgtccatgg tcaattcaca
     9481 ttctggctga ttgacccctt tctgatgaca ggctattgtc gtccatgcga tttttcatga
     9541 caggtttcag cctcctggcg ctt
//
LOCUS       DASTQY010000135         5859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1680119, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000135 DASTQY010000000
VERSION     DASTQY010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680119"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..2423)
                     /locus_tag="VFR82_04380"
     CDS             complement(72..2423)
                     /locus_tag="VFR82_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4500866.1"
                     /translation="MTTTHVTRRDTSPGNDARTLSSLRTKFVVFFSLILILTCSTLSW
                     YFIEVRRVSMTENLNQLGTILLTSVVNNGQFRYGGLIAEDRATLREFVESLMAVDDVV
                     YVVIRGADHIILAQQNKVVRESLGSITFSQERRYYPDEELAEEMYRTPTTMPRITRLA
                     LSQDKILVPDGGTSDTMWVLSALTERLYDFALPVLRKPATDPSLSPLLLEMEERIQSK
                     RSAAPYQVQGVLQIGITDAGVKRALLDMIRNVLVLTTLLIGAGILGAHFLTLRVTTPL
                     RRLAAVARQLAEGESPAPIPATTYDEVGQLTQTFNSMSRALQERSAAISVNMETIRKQ
                     ITQLTTVHQTSAAITSTLDLHALMDIVLKLLRSNLGFSRMVLMLRHEDRDVAYVAQVA
                     GVSDEIAEAARHLTVPIKEDGTILADLFIHAKPVLAREVPIVSHRMHPAILELARRVG
                     VQSFVCVPLQSHNQTLGFIAGDRGEQSCSEEDLNILLTIASHVATAIDNARTYANLAQ
                     LTQHLEQRIQERTRELSVANDRLQDHDRRRTMFVSVASHELRTPMTAIRSFADNMLDG
                     VAGALSDRQKTYLERIGHNLNRLTRIINQLLDWSRLDMQKEILRLESLDINQVATLVV
                     ESLRTVAAEKQVEIRIEAPEHLPMIHADRDKIEQILWNLIGNAVKFTPPTGLVTVRFD
                     ALPDGLVQTCVSDTGCGIPPDHLDRVFHEFSKVPSTMPGSQGAQLGLFITKSFITMHK
                     GEIRVESELGVGSRFFFTVPAVAPGAGGISPEIVSPGSSAKTA"
     gene            complement(2431..3390)
                     /locus_tag="VFR82_04385"
     CDS             complement(2431..3390)
                     /locus_tag="VFR82_04385"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4500867.1"
                     /translation="MASIGRLVHRADHVTSRLLVGAGCLSLYWASNIPPSVAAEIAIL
                     KSSGISAYDQAITGLKSTAPSGAIYSEYDVQGDLEQGRKLAKKIRASEASLVVAVGLK
                     AALAAKLEIVDIPIVYMMILDPSKHNLTAPNMTGTLLEIPAERQFKILRAFLPSLHRL
                     GMLSKVGGNQAKLKDTIAQAATQSFQLQEFPVESEKAVPQQLRSLLASTDALLLVPDS
                     TVLTNESVGFILETSLAKRVPVIGFSPEFTRLGALLSLSVSYGEVGRETGLLAKRILE
                     GDRKLPGKPVPVERLKITVNLKTARFLGIEFPKDVNHLIDETY"
     gene            complement(3437..5812)
                     /locus_tag="VFR82_04390"
     CDS             complement(3437..5812)
                     /locus_tag="VFR82_04390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4500868.1"
                     /translation="MVIPGGRYSILVVEDNPDIVIGLQDLLQHDGYDVSVAGTCAAAI
                     ALVGQQRFNAILLDLGLPDGDGLDVLREVQRRDPSLPVIIVTAHIAQERTVGSLVKGA
                     FAYLTKPYNREELRQNLRRAIGVKELAVKVERAEQSLTESEHRFQSLVESATDAIVVA
                     NGRGIIVSGNRSASRLFGYAVEEMIGQPLTILMPPRYREAHERGLARIEATGTGRLIG
                     SVVELHGLRKTGEEFPIELSLATWKTASGSYYSGIIRDISERKKAAHALEKLQRQHTL
                     ILNQAGEGIYGVDDRGNTTFVNPSGASMLGYRVDELLGRHMHEILHHSRPDGTPYPVD
                     ECPVYAAIQDGLVHRVVDEVFWRKDGTAFPVEYVSTPISESGNVVGAVVVFRDTTARR
                     ETELALKASEERLEMVIQGSTDGFWDGRVLPNEHWSSPRTPIWFSPRVKSMLGYTDEE
                     FPDVLESWATRLHPEDRERVFVALTAHIERRVPYDEEYRLLTKWGDYHWVRARGQAIW
                     DADGRVIRMSGSLQSIMERKRAEDALLRSQQLLHDMADNTIAVIYVKEASGRYILVNR
                     RFEEIFGLKAERIIGFTDHDLFPPDFADVFRANDVEVLSRNATVEYEEIAPHLDGPHT
                     YISIKFPLRDQAGIPYALCGISTDISERKQTENALRSHERQLRLALTSKQIGVWNWDI
                     ETDCMFWSPQVDEFLGMPPVPGHKTHRGLLALVHPDDREVMARAAKQVLDPFRTDIIF
                     EHRVKRSDGMMVSCIWTGHIVRDHAGKAVHVLGTVRATPAPNGDSGTKDFT"
BASE COUNT         1190 a         1701 c         1716 g         1252 t
ORIGIN      
        1 ctgaaaagtc ctggaggtcg gcatctcgtg tgtctccgac aacgcctaga cgagggtttc
       61 cctcgacgcg cctaggcagt tttcgcgctc gagcctggag agacgatttc gggcgagatg
      121 ccacctgccc ctggtgccac agccggcacg gtaaagaaaa agcgcgaacc gacccctaat
      181 tcgctctcga cccggatctc acccttatgc atcgtgatga agcttttagt gataaacaga
      241 ccgagttggg cgccttgcga ccccggcatc gtagaaggca cctttgagaa ctcatggaac
      301 acccgatcca gatgatcggg cgggatgcca caccccgtat ccgagacgca ggtttggacc
      361 aggccgtccg gcaacgcgtc gaaccggact gtcaccaatc ccgtgggtgg cgtgaacttc
      421 acggcgtttc caatgaggtt ccagaggatt tgctcaatct tgtcgcgatc agcatgaatc
      481 atgggcaggt gctccggtgc ctcaatgcgg atttccactt gcttttctgc tgccaccgtt
      541 cgcaagcttt caacgaccag cgtggcgacc tggttgatgt ctaatgactc gagccggaga
      601 atttccttct gcatgtccaa gcgcgaccaa tccagcagtt gattgatgat tctcgtcagc
      661 cggttcaggt tgtgcccgat ccgctcgaga taggtctttt gtcggtcgga gagcgccccg
      721 gccacgccgt ccaacatgtt gtcggcaaag ctgcggatgg ccgtcatcgg agtgcgcaat
      781 tcatgtgagg cgacagagac aaacatggtg cggcgtcggt catgatcttg aaggcggtcg
      841 ttggcaacgg agagttcacg ggtgcgctct tggatgcgct gctccagatg ttgggtcagt
      901 tgggccagat tggcatacgt gcgggcattg tcgatggcag tcgcgacgtg gctggcaatg
      961 gtgagcaaga tgttgagatc ttcttcggag cacgactgtt cgcctcgatc tcccgcgatg
     1021 aaccccaatg tctggttatg gctctgcaac ggcacgcaca caaacgactg cacgcccacg
     1081 cgacgtgcga gctcgaggat ggccggatgc atccgatggg agacgatggg tacctcccgc
     1141 gccaacaccg gcttcgcgtg aatgaagaga tcggccagaa tcgttccgtc ttccttgata
     1201 ggaacggtca aatgacgggc cgcctcggca atttcatccg agaccccagc cacttgcgcc
     1261 acgtacgcga catcacggtc ttcgtgtcga agcatcagga ccatacgtga gaaaccgagg
     1321 ttggagcgca acagcttgag cacgatatcc atcagcgcat gcagatccag ggtgctcgtg
     1381 atcgcggcgc tggtctggtg gaccgtcgtg agctgggtga tctgctttcg gatggtttcc
     1441 atgttgacgc tgatggcagc gctgcgttcc tgcagggccc ggctcatgct gttaaacgtt
     1501 tgggtcagct ggccgacttc gtcataggtc gtcgccggga tcggagccgg tgattcgccc
     1561 tcggccaact ggcgggcgac tgcagcgagc cttctcaatg gagtcgtcac ccgcagggtc
     1621 aggaaatgcg cgcccaagat gccggcacca atgagcagcg tcgtcaggac caggacgtta
     1681 cggatcatgt cgaggagcgc acgcttgacc cctgcgtcgg taatgccgat ttgcagcacg
     1741 ccttggacct ggtagggggc ggcgctcctt ttcgattgga tccgttcctc catttcaagc
     1801 agaagcgggg agagcgaggg atcggtcgcc ggcttgcgaa ggaccggcaa cgcgaaatca
     1861 tagagtcgtt cggtaagggc cgaaagcacc cacatggtat cggacgtgcc accgtcggga
     1921 accaggattt tgtcctgcga cagggcgaga cgcgtgatgc ggggcatggt tgtgggcgtg
     1981 cgatacattt cttcagccaa ctcttcatcg ggataatacc gacgctcctg cgagaaggta
     2041 atgcttccca atgattcacg gacgaccttg ttctgctgcg ccagaatgat atggtcggcc
     2101 cctcgaatca ccacatacac cacatcgtcg acagccatca ggctttcaac aaactcacga
     2161 agtgtggctc ggtcttccgc gattaagcct ccgtaccgga actggccgtt gttcacgacg
     2221 ctggtcaaga ggatcgtgcc taattggttg aggttctccg tcatcgagac ccgccgtacc
     2281 tcaatgaaat accagctgag tgttgagcag gtgaggatga ggatcaagct gaagaacacg
     2341 acgaacttcg tgcgaaggct gctgagcgtt cgtgcgtcat tgcccggcga agtatccctg
     2401 cgtgtcacgt gcgtggtcgt catggtcgat tcaataggtc tcatcgatca agtgattcac
     2461 gtctttggga aactcgatgc ccagaaaccg cgccgtcttg agattgacgg tgatcttcag
     2521 ccgttcgact ggaacgggtt tgcccggaag cttccgatcc ccctctagga tacgctttgc
     2581 caacaagcct gtctcccggc ccacttcccc gtaggacacg gaaagactca gcagggctcc
     2641 caaccgggtg aattctggcg aaaatccaat aacgggcacc cgcttcgcta aagacgtttc
     2701 caggatgaat ccgacggact cattggtcag caccgtcgaa tctggcacca gtaacagggc
     2761 gtccgtagaa gccagaagcg accggagctg ctgggggaca gctttctcgc tttcgacagg
     2821 gaactcttgc agttggaacg attgcgtggc ggcctgcgcg atggtgtcct tcagcttggc
     2881 ctggttgcca ccgaccttgg acaacatgcc aaggcgatgc aggctcggaa ggaatgcccg
     2941 caagatcttg aactggcgct ccgccggtat ttccagcagc gttccggtca tattgggagc
     3001 ggtcagatta tgcttggacg ggtccaatat catcatatac acgatgggaa tgtcgacaat
     3061 ctcgagcttg gccgccagcg cggctttcaa gcccaccgca actaccagcg acgcttcaga
     3121 tgcccgaatt tttttggcga gcttgcgccc ctgttccaga tcaccttgca cgtcatactc
     3181 ggaataaatg gccccggacg gcgccgtcga tttcaatccg gtgatggcct gatcgtaggc
     3241 actaatgccg gaggatttca ggatggcaat ttccgctgcc actgatggag ggatgtttga
     3301 ggcccagtac agggacaagc accccgctcc caccagcagg cggctcgtga cgtggtccgc
     3361 cctgtggacg aggcgcccta tagaggccaa gacattcatc gcaagccgga ggcctgtcga
     3421 gctcgagtca acgtcgtcat gtgaagtcct ttgtgccaga gtctccattc ggggcgggtg
     3481 tcgctcggac ggtgcccaac acatgcaccg ctttgccggc gtgatcgcgc acaatgtgtc
     3541 cggtccagat gcaggacacc atcataccgt cggatctctt gacgcgatgc tcgaagatga
     3601 tgtccgttct gaagggatcc aggacctgct tggcggcacg cgccatgacc tcgcgatcat
     3661 ccggatgcac caaggcgagc agtccacggt gcgtcttatg ccccgggaca ggcggcatgc
     3721 caaggaattc gtcgacctgg ggtgaccaga acatgcaatc ggtttcaatg tcccagttcc
     3781 agaccccgat ttgcttcgac gtcagcgcca gacggagttg ccgctcatgt gaccggaggg
     3841 cattttctgt ctgtttgcgt tcgctgatgt cggtggagat accacagagc gcgtagggaa
     3901 tcccggcctg atcccggagc ggaaacttga tcgaaatata cgtatgcggt ccatcgagat
     3961 gaggcgcgat ctcttcatat tctaccgtgg cattccgcga caatacttcg acgtcatttg
     4021 cgcgaaagac gtcggcgaaa tcagggggaa agaggtcatg atcggtaaat ccgatgatcc
     4081 gctccgcctt cagcccgaag atttcttcaa aacgtcggtt gaccaatatg tatcggccgc
     4141 tggcctcctt cacgtagatg acggcaatcg tgttatcggc catgtcatga aggagttgct
     4201 gactgcgaag cagcgcgtct tctgcgcgct tgcgctccat gatcgattgg agagagccgg
     4261 acatacgaat gaccctgcca tcggcatccc agatggcctg gccgcgggct ctcacccagt
     4321 gataatcgcc ccatttggta agcaggcgat actcctcgtc gtagggaacc cggcgttcga
     4381 tatgcgccgt gagcgctacg aacacgcgct cgcggtcttc ggggtgaagc cgtgtcgccc
     4441 agctctccaa cacgtcggga aattcctcat cggtatagcc gagcatgctt ttcacgcgtg
     4501 gcgaaaacca gatcggcgtg cgtggcgagc tccaatgctc gtttggcaag acccgaccat
     4561 cccagaagcc gtcggtcgat ccttgaatga ccatctccaa ccgctcctcg ctggccttca
     4621 atgccaactc ggtttctctt cttgcggtcg tatcgcggaa gaccaccacg gcgccgacga
     4681 cgttgccgga ttcactgatg ggagtgctga catattccac gggaaaagcc gtcccgtcct
     4741 tgcgccagaa gacttcgtcc actacccgat ggacaagccc atcctggatg gcggcataca
     4801 cagggcattc atcgacggga tagggggtgc cgtccggccg cgagtggtgc aggatttcgt
     4861 gcatgtgacg gcccaacagt tcatccaccc ggtatcccaa catgctggct ccactcgggt
     4921 tcacgaaggt cgtgtttccc cggtcatcga ccccgtaaat gccttctccg gcctggttca
     4981 gaatgagggt gtgctggcgt tggagctttt caagggcgtg cgcggccttc ttgcgttcgg
     5041 agatatcgcg aatgatgccg ctgtagtagc tgccgctggc agttttccag gtggcgagcg
     5101 acaattcgat cgggaattct tcaccggtct tccgaagccc gtgcaattcg accacggagc
     5161 cgatcaagcg gcctgtgccc gtcgcttcga tccgggccaa accccgttca tgggcttcac
     5221 ggtaacgcgg aggcatgagg atcgtcagcg gttgcccaat catctcttcc acggcataac
     5281 cgaacaaccg tgaagccgag cgatttcccg acacaatgat tccacgccca ttagcgacaa
     5341 cgatggcgtc agtcgccgac tcgacgagcg actggaaccg gtgctcgctc tcggtcaacg
     5401 attgctctgc tcgctcaact ttgacggcga gctctttgac gccgatcgcg cgccgaaggt
     5461 tttgtctgag ttcctctcgg ttatacggct tcgtgaggta ggcgaacgcc cctttcacca
     5521 gtgatccgac ggtgcgttct tgggcgatgt gcgcggtgac gatgatgacc ggcagggatg
     5581 gatcccgacg ttgtacttct ctcaggacgt ctaggccgtc cccatcaggc aatccgagat
     5641 cgaggagaat cgcattgaaa cgctgttggc ctacgagcgc gatcgcagcc gcacaggtgc
     5701 cagccacgct gacgtcatag ccgtcgtgct gcagtaaatc ctgcagtccg atgacgatat
     5761 ccgggttgtc ctccaccacc aggatggagt aacgcccgcc cggaatgacc atcactccct
     5821 cagctgagac actgtgtagg tctctctaca tacgtagcg
//
LOCUS       DASTQY010000136        11318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1422536, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000136 DASTQY010000000
VERSION     DASTQY010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11318
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422536"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1576
                     /locus_tag="VFR82_04395"
     CDS             <1..1576
                     /locus_tag="VFR82_04395"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEU4500869.1"
                     /translation="TSQTAPPEEVCARAERTLDRAVAAGFPALLSSQGQYMDDFWQRS
                     DVKVMTNPVRAKASTTAVQQAIRFNLFHVLQASARAENTGVPAKGLTGSAYEGHYFWD
                     SEIYVLPFLTYTAPRIAKNLLRFRHGMLDKARERARQLNQRGAMYPWRTINGEEASAY
                     YAAGTAQYHINADIMYALKKYIQATDEEEFLFEEGVEMLVETARLWRSLGYYSPLKGE
                     QFSIHCVTGPDEYNTVVDNNVYTNLMAKENLLYAAETVERVKQRRPDLYTALVHKTRL
                     EPSEVTEWKEAADRMYIPYDERLKINPQDDHFLEQEPWDFDRTPADKYPLLLYFHPLT
                     IYRHQVIKQADLVLAMFLLGHEFAPDLKRRNFAFYDPITTGDSSLSACIQSIMAAEVD
                     EMDKALEYAKVATLMDLGDVAGNVKDGCHIAAMGGVWMVFVYGFAGLRDYDGELCFWP
                     SLPEVFNCIRFNLIFKGQTLEVDVGHQVTHYTLKSGKGLAIRHEGQVFQLSPGAPSVQ
                     QPNGRPSKDGALKKGA"
     gene            complement(1589..2233)
                     /locus_tag="VFR82_04400"
     CDS             complement(1589..2233)
                     /locus_tag="VFR82_04400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4500870.1"
                     /translation="MGELVPAEIEAYAAEHSMEESELCRELRAETHRHMESPQMLVGP
                     LEGALLKMLTQLVRATHVLEIGTFTGYSALCFAEALPDHGTVTTCEIDEASAALARRY
                     VARSPHGVKISIRMGPAIETMRTLKRQYDVIFIDADKLNYLNYYLRAKELLTPEGVIL
                     IDNVLWDGDVLVTPAPDERTAAIQAVNRVVASDPEMVAVLLTVRDGVLLIRRKV"
     gene            2336..3868
                     /locus_tag="VFR82_04405"
     CDS             2336..3868
                     /locus_tag="VFR82_04405"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEU4500871.1"
                     /translation="MKTLRTLESLARRLLEIQRVNSAASVLSWDQETYMPAGGGAARA
                     EQIATLQGIAHQILVSPETERLLGGWLDLQTGLPLEQDGQLWDESSRALLREVWRDYS
                     RAKKLPSDFVMQLSRECSLAQQVWAEAKAQNNFKSFLPSFRTVLSLKREEAQFLGFSG
                     SPYNALLDSYEPGSTVASLAPLFTQMKARLVPLLTKIQQSRLQIDDSFLYHAYDQARQ
                     LEFGRLVLIAMGYDFEKGRLDLSAHPFTTSFHPTDVRVTTRVHEHDLPSCLFSCIHEG
                     GHGLYDQGLDPAHYGTPLGESVSLGIHESQSRMWENCVGRSRAFWRFFYPILQATFHH
                     QLRAVNMDQFYAVINRVKPSLIRVEADELTYNLHIMLRFEIEQDLIEGNTNPEELPAI
                     WNLKMKEYLGVSPSNDAEGVLQDVHWSMGGFGYFPTYTLGNLYAVQFYEQAKLEIPHL
                     EDEIAAGQLMVLRRWLAQKIHRWGRMFPPEHLSQRVTGVSLSPEPFLRYIDRKYSELY
                     HL"
     gene            complement(3889..4452)
                     /locus_tag="VFR82_04410"
     CDS             complement(3889..4452)
                     /locus_tag="VFR82_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500872.1"
                     /translation="MRTPHQITVDRALLLYVLHLAEPHGLLSDVKLQQLCFLCELQMF
                     GKGLKGFHFEFFRFAYGAFSKDLDNDLTSLRRKERVENFTLADQTQEKVLPLIEEGVA
                     GVETNEKILEILKAVMTTYGPQDSGTITSSVEAVELSTPQDPEFKIPIGDIVFHTTLL
                     VPQRIDVQAEFTLPPALLSRVNQAMGY"
     gene            4584..5021
                     /locus_tag="VFR82_04415"
     CDS             4584..5021
                     /locus_tag="VFR82_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEU4500873.1"
                     /translation="MGSGKFAKEMNVLKEHLGKHQLKFTRQREMILTAFLRQEHITAE
                     AMYHQLAKTDPHLGLATIYRTLNLFCDAGIAQARHFGSQTQYDNVSHKGHHDHLICTG
                     CGKIVEFENCEIEKLQEEVATRNGFTIQTHRLELYGLCSRCRH"
     gene            5083..6099
                     /locus_tag="VFR82_04420"
     CDS             5083..6099
                     /locus_tag="VFR82_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU4500874.1"
                     /translation="MTIRIGFLALACLLTTFLQAVSAPSAEKLTVYSGRAERLIKPVL
                     DEFSAKTGVQVELLSSGTAELVNRLKAEGDRTPADLFLTNDAGSLEQTRAAGVLRPLS
                     MGEVERAIPPQFRAPDNAWIGLSGRFWIIVYNKMLVKPGELKSLLDLGDPKWKDKIAI
                     PNAGSEYLQAGVSVIRATHGDAKTKQFLEGLKKNAGGQVYQKSSQIVEAVAKGQASLG
                     IVNHYYVYRHLAAQPSAPIAAFMPDQQEAGMGAIMNVAGVGIVKSTKHLETAKLLVEF
                     LVAQAGQKLFADLDKEYPLHPDVKADPALVERKSFRVAQVPLTRLAELREPTLTLIEE
                     VGLR"
     gene            6265..7848
                     /locus_tag="VFR82_04425"
     CDS             6265..7848
                     /locus_tag="VFR82_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU4500875.1"
                     /translation="MTTSRRQLTSPLQLMTLVTATLILLPLGYVMSQALSADPAVWSR
                     LWNTRIPELLTNTIRLAASVACFTLVLGVSTAWLVTRVEFPARRLWEGALVLPLAMPT
                     YVLAYIYSYLLGYGGPIEQLWQTVAGPQARVFSPHSFLGATLVMTLDTFPFVYLLSRS
                     ALLSMNSSFEEVSRASGISRMKTLWLVTLPLMRPSIAAGLALVILYVVSDFGAVSLLR
                     YQTLTYAVFQQMTGRSDTTAASILSLLLVGMALLFLVTERWFRHRSRYYQTTGRYRTP
                     ERHRYGWMGTSLVTIYLVLVVAASFGVPAYLLIDWSLSPEALATIDTRFYSFFGNTAF
                     LAACAATGGVVMGLPLAYMASRRPNWLNLGCLQAAYAGYVLPGPVAALAVLVLCLNLV
                     PFVYGSVIVLIVAYIIHFLPAALQSLEPALQQITPNLEEVARTLGLGIRQTWRRVTLP
                     LIRSGFVVAWVLMFLQTMKELPATLLLRPVGFDTLAIRVWMEASEEYYQLAAPSALLI
                     VLLGMPTLLLLLSKDWRAA"
     gene            7899..8981
                     /locus_tag="VFR82_04430"
     CDS             7899..8981
                     /locus_tag="VFR82_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4500876.1"
                     /translation="MLQADLYQPVSSVLELRGVSCDYGAGRPAVENIAFAAKEGEILC
                     LLGPSGCGKTTILRAIAGFEPLRSGQIFLSGHLVSSPDYMTPTEDRHVGMVFQEYALF
                     PHLRVQDNIAFGLRHLSRRERKVRVHDMLRLVGLEGFERRYPHELSGGQQQRVALARA
                     LVQHPVVLLLDEPFSNLDPDMASRMRQELHDLLRRTKTTTILVTHDHDEAFAMADRIA
                     VLNQGKLEQFDTPETIYHMPQTPFVADFVGQADFIPGTVTKGTVKTELGEFPNTLECE
                     EGTAVVVMIRPDDVHLASDRNSDVRVVSRQFRGSENLYAVALPSGQVVHSSQGSTVVL
                     PLGTPVRLSIVATHTVIFAPIGVQNS"
     gene            9090..9515
                     /gene="exbB"
                     /locus_tag="VFR82_04435"
     CDS             9090..9515
                     /gene="exbB"
                     /locus_tag="VFR82_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEU4500877.1"
                     /translation="MDSLKHAVDFGVIGLLLVLSVWCIAVAVERWMFYRRIDLKQFPS
                     QQIAEIALTKRLVVIGTVAANAPYIGLLGTVLGIMLTFHTMGTSGQMAVSTIMIGLSL
                     ALKATAVGLLVAIPCVVMNNILRRRVSELLTLYKAQHGT"
     gene            9505..9879
                     /locus_tag="VFR82_04440"
     CDS             9505..9879
                     /locus_tag="VFR82_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU4500878.1"
                     /translation="MEREIDQINVIPLVDVMLVLLVIVMTTATFITTGHIPVDLAKAK
                     EAGDRKDVPIVITLAADGGLFLNDQSVSPESLKSQLTPHPKETLVLVRADRVTVLERF
                     VNVVDDVRGLGFHQVSLEVVRS"
     gene            9876..10913
                     /locus_tag="VFR82_04445"
     CDS             9876..10913
                     /locus_tag="VFR82_04445"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU4500879.1"
                     /translation="MTAAALGQFGHSSHKHHGWIASMLIHGLAVSLSLFLFAELKPAA
                     QPEPFRWEIALAQVASPSPQVEPPAPVPPQPKRVVQTPSPAPPKPVETKPVVQRVQTV
                     QTVQRVESVVRQEEMAVQQINPVVESIAQHAEPVRQERQTERTTEAVQRAEAIEVPVA
                     AVMVPTPVSQPTPISPPEPAQGVPEVITHHEPVTTNEPVIAEAPVLNQQPAVNHSAPE
                     SVEAPAPVVQTARVAPSQALVERPAMVTAQTRADYSWLTHELISRLEQSKRYPYAARM
                     NRWEGRVVVQAVVRDDGQVISLKIAESSGHAILDNDAMELIRKISPLHLKHPLGQSQV
                     ALLVPVGYALR"
     gene            10938..>11318
                     /locus_tag="VFR82_04450"
     CDS             10938..>11318
                     /locus_tag="VFR82_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500880.1"
                     /translation="MRAFSRMPLIGLVGLILALIIPSAVFSETKADRQFHATVTDGQG
                     IETEIKNIMFYWEEKVSDTAFVPHELRHVPVKKGTATVNVKFDNIKQIEVKPSADSGL
                     PSLAITLTNGKTGEFALAIKGSFRG"
BASE COUNT         2460 a         3206 c         3123 g         2529 t
ORIGIN      
        1 cacgagtcag acggcgccgc ccgaggaagt atgtgcgcga gccgaacgca cactcgatcg
       61 ggcggttgcc gcaggatttc ccgccttgct tagctcgcaa gggcagtaca tggacgactt
      121 ctggcaacga agcgacgtca aggtcatgac caatcccgtc cgggcgaaag cgtcaacgac
      181 ggccgttcag caggcgattc gcttcaatct ctttcatgtg ttgcaagcca gtgcgcgtgc
      241 ggaaaatacc ggcgttcccg cgaaaggact aaccggatcg gcctatgagg gacattattt
      301 ttgggacagc gaaatttatg tgctgccctt tttaacctac acggcgccgc gaatcgcgaa
      361 aaatctcttg cggtttcgcc acggcatgtt ggacaaggcg cgcgagcggg cgcgacaact
      421 gaaccaacgg ggggcgatgt atccctggag aacgatcaac ggtgaagagg cgtccgccta
      481 ctacgcagca gggaccgcac agtatcacat caacgccgac atcatgtatg cattgaaaaa
      541 atacatccag gcaacggacg aggaggagtt tctcttcgaa gaaggagtcg agatgctggt
      601 ggagaccgct cggctatgga ggtcgctcgg gtactattcg ccccttaaag gcgaacaatt
      661 tagcattcac tgtgtcacgg gtccggacga atacaatacc gtggtggaca acaatgtgta
      721 taccaatctg atggcgaaag aaaatcttct ctacgcagcc gagacggtcg agcgcgtcaa
      781 gcaacgcaga ccggatctgt atacggccct ggtgcataag acgcggttgg agccgtcaga
      841 agtgacggag tggaaggagg cagccgatcg gatgtacatc ccctacgatg aacggctcaa
      901 gatcaaccca caagacgatc actttttgga acaagagcca tgggatttcg atcggacacc
      961 ggcagacaaa tatccgctgc ttctttattt ccacccgctg accatctatc gccatcaagt
     1021 catcaagcag gcagacctgg ttttggccat gtttttgcta ggccacgagt ttgctcctga
     1081 cctgaaacgc cgaaacttcg cgttctatga tccgataacg accggcgact cttcgctttc
     1141 cgcttgcatc caaagcatta tggctgccga agtcgacgag atggacaagg ccttggaata
     1201 tgccaaagtg gcgacgctga tggacctggg ggatgtggca ggaaatgtca aagatgggtg
     1261 ccatatcgcc gccatgggcg gagtctggat ggtattcgtc tatggctttg ccggcttgcg
     1321 cgattacgac ggcgaactgt gcttctggcc gagcttgccg gaagtgttta actgtatccg
     1381 gttcaacctg atctttaaag gccaaacact ggaagtggat gtcggccacc aggtgacgca
     1441 ttacacgctg aagagcggca aggggctggc gatccgccat gaaggtcagg tgttccaact
     1501 gtcgcctgga gcgccatccg tccaacagcc gaatggtcgg ccctcgaaag atggtgcgtt
     1561 gaagaagggc gcatgaatag cgctcatctt aaacttttct tcggatgaga agcacgccat
     1621 cccgcacggt gagaagcaca gctaccatct ccggatcgga agccaccacg cgattcactg
     1681 cctgaatggc agccgttcgt tcgtccggcg ccggcgtgac caggacgtct ccatcccaca
     1741 acacattgtc aatcaagatg actccttccg gcgtgagcaa ctctttcgca cgcagataat
     1801 agttcaggta gttcaacttg tcggcgtcga tgaagataac atcatactgc ctcttgagtg
     1861 tccgcatcgt ctcaatggcc gggcccatgc ggatggaaat cttgacccca tgcggtgagc
     1921 gagcaacata gcggcgggct agcgcggcag atgcctcgtc gatttcgcag gtggtcaccg
     1981 tgccgtgatc gggcaatgct tcggcaaaac acagggcgct gtatcccgta aacgtgccga
     2041 tttcaaggac gtgggtcgcg cggaccaact gggtcagcat tttcagtagg gccccttcca
     2101 ggggaccgac caacatctgc ggagactcca tgtgccgatg cgtttccgca cggagttcac
     2161 ggcagagctc agactcctcc atcgaatgtt cggcggcata cgcttcgatc tctgcgggaa
     2221 ccaattcccc catagtccct cctcggtttg aaaatcggta cggtggtgcg tacactgcat
     2281 gaagagacgt cgattaggct cgatcatagc acgtagggcg gaggaggcgg tcatcatgaa
     2341 aaccctcagg acacttgaat cgttggcccg ccggttgctg gagatccagc gtgtcaacag
     2401 tgcggcctct gtgctctcgt gggaccagga aacgtacatg ccggcaggcg gcggtgcggc
     2461 ccgagccgaa cagattgcaa cccttcaagg cattgcccac caaatactgg tttcgcccga
     2521 gacggaacgg ctactcggcg gttggctcga cctgcagacc ggtctccccc tagagcagga
     2581 tggccaactg tgggacgaat cgtctcgcgc tctgctgcgc gaagtgtggc gggactacag
     2641 ccgtgccaaa aaattgccgt ccgattttgt tatgcagttg agccgcgagt gctccttggc
     2701 gcaacaggta tgggccgaag ccaaagcaca aaacaatttc aaatcgttct tacccagctt
     2761 tcgaacagtc ttgtcgctga aacgcgagga agcacagttt ctgggatttt ctggttcccc
     2821 ctacaatgcg ctgctggact cctacgagcc tggatcaacg gtggcgtcgc tggcgcctct
     2881 gttcacacag atgaaggccc gactggtccc actgctcacg aaaattcagc agagtcggct
     2941 ccagatcgac gacagttttc tctatcatgc ttacgatcag gcgcggcagt tggaattcgg
     3001 ccggttggtg cttattgcca tgggatatga tttcgagaag ggccggctgg atctttccgc
     3061 acatcctttc acgacgtcgt ttcaccccac tgacgtgcgc gtcactactc gggtccatga
     3121 gcacgatctc ccgtcgtgcc ttttcagctg catccacgaa ggaggccatg ggttgtacga
     3181 ccaaggcctc gatccagcgc attacggcac gccgctcggc gaatcggtgt cgctgggcat
     3241 tcatgagagt cagtcgcgta tgtgggaaaa ttgcgtgggc cgttcacgtg ccttctggcg
     3301 ttttttctac ccgatcctcc aagcgacgtt ccatcatcaa ttgcgcgcgg tgaacatgga
     3361 ccagttctat gccgtcatca accgtgtcaa accctcgtta atccgcgtcg aagccgatga
     3421 gttgacgtac aacctgcaca tcatgctgcg atttgagatc gagcaggacc tgatcgaggg
     3481 caacacgaac cctgaagagc tgcctgccat ttggaattta aagatgaagg aatacttagg
     3541 cgtttctccg tcgaacgacg ccgaaggcgt gctgcaagac gtgcattggt ccatgggcgg
     3601 cttcggctat ttcccgactt acacgttagg gaacctgtat gccgtgcagt tctacgagca
     3661 ggccaaactg gagattccac acctagagga tgaaatcgcg gccggacagt tgatggtctt
     3721 gcgaaggtgg ctcgctcaaa aaatccatcg gtgggggcgc atgtttccgc ctgagcatct
     3781 gtcccaacgt gtgaccggcg tcagtttaag tccggagcca ttcctgcgct acatcgatcg
     3841 gaagtacagt gaactgtatc acctgtgacc gagccttgcg tgagtgcgct agtatcccat
     3901 cgcctgattc actcgtgaca gcagggccgg tggcaatgtg aattccgcct gcacgtcgat
     3961 gcgctgaggc accagcagtg tggtgtggaa aacgatgtcg ccaatgggaa ttttaaattc
     4021 cggatcctgg ggagtgctga gttcgaccgc ttcaacagag gaggtaattg tgccgctgtc
     4081 ctgagggccg taggtcgtca tcaccgcctt gagaatctct aagatttttt cattggtctc
     4141 aacgccggcc acaccctcct cgataagtgg aagaactttt tcctgcgtct gatcggcaag
     4201 agtaaagttc tccactcgtt ccttacgccg gagcgaggtc agatcattgt cgagatcctt
     4261 actgaacgcc ccgtaggcga accggaaaaa ttcgaaatga aaccctttga ggcccttgcc
     4321 gaacatctgc agttcgcaca agaaacacag ttgctgaagc ttgacgtcgc tcaacagtcc
     4381 atggggctcg gcgagatgca ggacatagag cagcaaagcc cggtcgacgg tgatctggtg
     4441 cggtgtgcgc atctgttaac agccttacac aaagtcaggc ggcgcagcac tataaacagc
     4501 tccgcaaaac cgcgtcaatt acacgcccct tgaccaaaca gcgcgttttc gtttttaatg
     4561 accaccggtc ggaggcgttc cgcatggggt ctggaaaatt cgcgaaagaa atgaatgtgc
     4621 tcaaggagca tcttggtaag catcagctca agtttacgcg tcagcgggaa atgattctga
     4681 ctgcgttctt acggcaggaa cacattacag ccgaagccat gtatcatcaa ctcgccaaga
     4741 ccgacccgca tttgggcctc gctaccatct atcggaccct gaatttgttt tgcgacgccg
     4801 gcatagcgca agcccgccat tttggctctc agacacagta tgacaacgtg tcgcataaag
     4861 gtcatcacga ccacctgatc tgcacagggt gcggcaagat cgttgaattt gaaaattgcg
     4921 aaattgagaa gcttcaggaa gaagtcgcca cgcgcaacgg cttcacgatc caaactcacc
     4981 ggctggaact ctacggcctc tgttctcgct gccgtcattg acgatcgccg agtctttttg
     5041 atatcgtctt gagatggctt atcaatctca attaaggaca ggatgacgat ccgcataggt
     5101 ttccttgcgt tggcatgcct cctgaccacc ttccttcagg cggtttctgc tccatcagca
     5161 gaaaaactca ccgtgtattc gggacgcgcg gaacgactca ttaagcctgt gctggacgag
     5221 ttcagcgcca aaaccggcgt acaggtggag ttgctctctt ccggaacggc cgagttggtc
     5281 aaccgcctca aggcagaggg cgatcgcacc cccgcagatc tgttcctcac gaatgatgct
     5341 ggcagtctag agcagactcg cgcggccggt gttctgcgcc cgttgagcat gggcgaagtc
     5401 gagcgggcga tacctcctca attcagagcg ccggataacg cctggatcgg cctgtcgggc
     5461 cggttttgga ttattgtcta taacaagatg ctcgtgaagc caggtgagct gaagtccttg
     5521 ctcgacctcg gcgatccgaa gtggaaggac aagatcgcca ttccgaacgc ggggagcgag
     5581 tacttgcaag ccggcgtatc cgtcatccgg gcgacacacg gcgacgcaaa aaccaagcaa
     5641 tttctcgagg ggctcaagaa gaacgccgga ggccaggtgt atcagaaaag ttcccaaatc
     5701 gtggaagcgg tcgccaaagg gcaggcctcg ctcggtatcg tcaaccatta ctatgtctac
     5761 cgccacctcg cggcacagcc gtcggcgccg attgctgcgt tcatgcccga tcaacaggag
     5821 gcaggcatgg gcgcgatcat gaatgtcgcc ggggtgggaa tcgtgaaatc aaccaaacat
     5881 ttggaaaccg ccaagcttct cgtggaattt ctcgtagcac aagcgggcca gaagctgttc
     5941 gccgatttag acaaggaata ccccttgcat ccagatgtca aggccgatcc tgccctcgta
     6001 gaacgaaaat cttttcgcgt cgctcaagtc cctctaacaa ggctcgctga gttgcgtgag
     6061 ccaacactga cccttattga ggaggttggt cttcgataag ctactgtccc ggacgcgttt
     6121 ctccttatcg acgatttctc ttgcgccctc gtctggtctg attcttgtct gtgctcagga
     6181 acgtcctcgt acagcaacac acgtgtcgaa gcgtctttca cacatccagt tgagtcatct
     6241 ttcacacatc caagtgagtc tttagtgacc acaagccgcc gccagctgac gtcgcctctt
     6301 caactcatga cgctggtcac cgcgacgctt attctgctgc cgctgggcta tgtgatgtct
     6361 caggcactat ctgccgatcc ggccgtctgg agccggctat ggaacacacg tatccccgag
     6421 ttgctgacta acacgatccg actggctgca agcgtagcct gctttacgtt ggtgctcggc
     6481 gtctccaccg cctggctggt cacccgggtt gaatttcccg ctcgccgact gtgggaaggt
     6541 gcactcgtgc tgccattggc catgccgact tacgtcttgg cgtatattta ttcgtatctc
     6601 ctcggatacg gcgggccgat cgagcaactc tggcagaccg tggccggccc tcaggcgcgc
     6661 gtcttctcgc cccacagttt tctgggtgcc acactcgtga tgacgctgga tacgtttccc
     6721 ttcgtgtatc tgctgagccg aagtgccctg ctcagcatga acagctcatt cgaagaagtg
     6781 tctcgtgcca gcggcatttc gcggatgaaa acgttgtggc tggtgacgct tccactgatg
     6841 cgtccgtcca tcgccgcggg gctcgcactg gttattctct atgtggtgtc cgattttggt
     6901 gcggtttcat tgctgcgcta ccagaccttg acctatgcag tgttccagca gatgaccggc
     6961 cgttcagaca cgactgcggc cagcattctc agccttctgc tcgtgggaat ggcgctcttg
     7021 tttctggtca ccgaacggtg gttccgccat cgcagtcgtt attaccaaac caccggacgg
     7081 tatcgcacgc ctgagcgcca tcgatatggc tggatgggaa catcgctggt cacgatctac
     7141 ctggtgctcg tcgtcgcggc atcctttggg gtaccggcct atctcctgat cgactggagc
     7201 ctttcacccg aagcgctcgc cacgatcgat acccgcttct acagcttctt cgggaatacc
     7261 gcctttctcg ccgcttgcgc ggcgacaggc ggcgtagtga tgggtctgcc gttggcctat
     7321 atggcaagtc gccgtccgaa ctggctgaac ctcggttgct tgcaagccgc ctacgccggc
     7381 tatgtgctcc ctggaccggt ggccgccctg gcggtactgg ttttgtgctt gaacctcgtc
     7441 cccttcgtgt acggctcggt catcgtcctt atcgtggcgt acatcattca cttcctcccg
     7501 gctgcactgc agtccctcga accggcgctg cagcaaatca ctcccaacct tgaagaagtc
     7561 gcccgaacgc tcggtctggg cattcgccag acttggcgac gcgtgacctt gccgctgatc
     7621 cgcagtgggt tcgtcgtggc ctgggtactc atgtttttgc aaacgatgaa agagctgcca
     7681 gcaaccttgt tgttgcggcc ggttggattc gacacgctgg ctattcgagt gtggatggag
     7741 gccagtgaag agtactatca attggcggcg ccatcggcgc tgctgattgt gctcctcgga
     7801 atgccgacct tgctgttatt attgtcgaaa gattggcgcg cggcctaaaa gaaggaagct
     7861 gcaacgtgac gcctcggcat gccataacag gagaatccat gctgcaagcc gacctttacc
     7921 agccggtctc gtcggtgctg gaactccgcg gcgtctcttg tgactatggc gccggacgcc
     7981 cagccgtaga gaacatcgca ttcgcagcca aagaaggaga aatcctctgt ctattgggtc
     8041 cgtcaggctg cggcaagacc accatcctgc gtgccatcgc cggcttcgag ccgcttcggt
     8101 ccgggcagat cttcctgtct gggcacctgg tgtcgtcgcc cgattacatg acaccgaccg
     8161 aagaccgaca cgtcggaatg gtctttcagg agtatgcgtt gtttccgcac ttgcgcgttc
     8221 aggataacat tgcattcggc cttcgccacc tcagccggcg tgagcgaaaa gtccgtgtcc
     8281 acgatatgtt gcgccttgtc ggcctggagg ggttcgaacg ccggtaccca cacgaattgt
     8341 ccggcggcca gcagcagcgc gtggctcttg ccagggcgtt ggtccaacac cccgtcgtcc
     8401 tgctgcttga cgaacccttc agcaatttgg acccggatat ggccagccgc atgcgtcagg
     8461 agttacacga tctcctgcgc cgaacgaaaa caaccacgat attggtgacg catgaccatg
     8521 acgaagcttt tgcgatggct gaccgcatcg ccgtactcaa ccagggcaaa ctcgaacaat
     8581 tcgatacgcc ggagaccatt tatcacatgc cgcaaacgcc gttcgtagcc gatttcgttg
     8641 gacaagcgga cttcattccc ggcaccgtga cgaaaggtac ggtgaagacc gagctggggg
     8701 aattcccaaa tacgctcgaa tgtgaagaag gcacggcggt cgtcgtgatg atcagaccgg
     8761 acgatgtgca tttggcgagt gataggaatt cagacgtgcg agtggtgagc cgtcagttcc
     8821 ggggttccga gaacctctac gctgtcgctc taccgtctgg acaagtcgtc cattctagcc
     8881 aaggctcgac agtggtcctt ccgctgggca caccggtgcg tctcagcatc gttgccacac
     8941 ataccgtcat ctttgctccc atcggcgttc aaaattcata atcacattcc ccgaactttt
     9001 tcattgttga caccagactc tccactttgc tatttttgca ttctaaagtt gagaacggtt
     9061 ttcaaattca aattggcagg tacatagtta tggatagcct gaaacatgcg gtagattttg
     9121 gagttattgg gcttcttctc gtactcagtg tgtggtgtat tgccgtcgcc gtcgaacgat
     9181 ggatgttcta tcggcgcatc gatttaaagc aatttcctag ccagcagatt gctgagattg
     9241 ctttgactaa acgactcgtc gtaatcggca ccgttgccgc aaatgccccc tacatcggtc
     9301 tcctgggcac tgtcctcggc attatgttga ccttccacac gatggggacg tcaggacaaa
     9361 tggcggtgag caccatcatg ataggcctaa gcctcgcact gaaggccacc gccgtaggct
     9421 tactcgtcgc cataccctgt gttgtgatga ataacatttt gcgccggcgg gtttcagagc
     9481 tcctcacgct ctataaagcg caacatggaa cgtgaaattg atcaaatcaa cgtcatccct
     9541 ctggtcgacg tgatgctcgt cctgctggtt attgtcatga ctactgcgac gtttattacg
     9601 acagggcata tccccgttga tctggcaaag gcgaaagagg ccggggaccg aaaagatgta
     9661 cctatagtga tcacgctggc agcggacggc ggtctatttc ttaacgacca atcggtctca
     9721 ccggaaagtt tgaagagcca acttacgcct cacccgaaag agacattggt ccttgtccgg
     9781 gctgatcgtg tcacagtgct cgaacgcttc gtgaatgtag tggatgacgt acgagggctt
     9841 ggttttcatc aggtcagcct ggaggtagtg cgctcatgac cgctgccgca ttgggacagt
     9901 ttgggcactc cagccacaaa catcacggct ggatcgcgtc catgctcatc catgggctgg
     9961 ctgtcagcct atcgttgttt ctcttcgcgg agctcaagcc tgccgcgcag ccggaaccgt
    10021 tccgttggga gattgctctc gctcaagtcg cgtctccttc acctcaagtg gagccgcctg
    10081 cgccggtacc gccgcagccg aaacgcgttg tgcaaactcc gtctccggcc ccacccaaac
    10141 ctgttgagac aaaacctgtg gttcagcgcg tgcagaccgt ccagacggtc cagagagttg
    10201 agtcggtcgt cagacaggaa gaaatggccg ttcaacaaat caacccagtc gtcgaaagca
    10261 tcgcgcagca cgcagaaccg gtgcggcaag agcgtcagac cgaacgcacc accgaagcgg
    10321 ttcaacgagc cgaggctatt gaggttccgg ttgccgccgt gatggttcct actccggtca
    10381 gccaaccgac accaatcagc ccacctgagc cggcgcaagg agtcccggaa gtcatcactc
    10441 accatgaacc agtcacaact aatgagccgg tgatcgcaga ggcaccggtg ctgaatcaac
    10501 agcctgctgt gaaccattcc gctcctgagt cagtcgaagc ccctgctcca gtagtccaga
    10561 cggcacgagt agccccttcg caagctttgg ttgaacgtcc ggccatggtc acagctcaga
    10621 cgagggcgga ttactcctgg cttacgcacg agctcatatc ccgcttggag caatcaaagc
    10681 gctatcctta cgccgctcgt atgaatcgct gggagggacg ggttgttgtc caagctgtag
    10741 tgcgggatga tgggcaggtg atcagcctta aaatcgctga gagctctggg catgccattc
    10801 tggacaacga tgccatggag cttattcgaa aaatctctcc cctccacctg aaacaccctt
    10861 tggggcagtc ccaagtcgcg cttctagtac ctgttggcta tgcgctgcga tagatttagg
    10921 tagatggagg aggacacgtg cgagcttttt cacggatgcc gttgattgga ctggtcggct
    10981 taattctcgc ccttatcatt ccctcagccg tgttctcgga gacgaaggcc gaccgtcaat
    11041 tccacgcgac cgtcacggac ggccaaggga tcgaaaccga gatcaaaaat ataatgtttt
    11101 attgggagga gaaggtcagt gacactgcct tcgttcctca tgaattgcgc cacgttcccg
    11161 tgaaaaaggg cacagcgacc gtgaacgtta aattcgacaa catcaaacag atcgaggtca
    11221 aaccaagcgc ggattcgggc ttaccgtcac tggccatcac actcaccaat gggaagactg
    11281 gggagtttgc cctcgccatc aaaggaagct ttcgtggc
//
LOCUS       DASTQY010000137         3938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1457124, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000137 DASTQY010000000
VERSION     DASTQY010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457124"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFR82_04455"
     CDS             complement(<1..282)
                     /locus_tag="VFR82_04455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU4500881.1"
                     /translation="MSLQQLAERLQEHFAVGFVKAVEWRGDLAITVTRDKLHEVAQFL
                     HDDPAMDFDYIVHVSSVDWPDDEERFEVVYEFYSIRKRHRIRLKTRVPES"
     gene            complement(289..816)
                     /locus_tag="VFR82_04460"
     CDS             complement(289..816)
                     /locus_tag="VFR82_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEU4500882.1"
                     /translation="MGLIQLGRHDKDGMPDVITTSVEKAVNWARKGSLWPMTFGLACC
                     AIEMIAAVSSRYDMDRYGAGVFRASPRQSDLMIVAGTVCRRMAPVIRKIYDQMPEPKY
                     VIAMGSCATSGNIYDSYSVVQGVDRFVPVDIYVPGCPPTPEALFDGILKLQERIMQKR
                     VFVKQPEQVRASLKA"
     gene            complement(807..1199)
                     /gene="ndhC"
                     /locus_tag="VFR82_04465"
     CDS             complement(807..1199)
                     /gene="ndhC"
                     /locus_tag="VFR82_04465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU4500883.1"
                     /translation="MSGLELLLEYLTKYFPILLFIFIALTFGVVTLLLSYLVQPKYPE
                     PEKLSAYECGSEPFSDARMPFPVRYYIFAMLFVIFDIEVIFLYPWAVVFNDINKVSVI
                     GLVEMLIFIALFLVAYVYAWRKGALEWD"
     gene            1654..3144
                     /locus_tag="VFR82_04470"
     CDS             1654..3144
                     /locus_tag="VFR82_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4500884.1"
                     /translation="MTTLPSRTFFGCLLLAVFSLTGLPHAVADELDTIRYGEAARTYA
                     AGSIQQSTPQDGVVNVITGDNQTVGNRMRLGQNDVLYLKLKHPGEAAVGDLYTVYKRT
                     RKVFHPATGQYLGYLITRLAIVQVVQVDKELTTVRALRAFGAAAPGDPVMKFALPHSD
                     EAAVIPASSDVTGMVVELQADLQMTLVAQRNIVYLDRGSDDGLKPGDRMDLIRYGGAL
                     PPRQVGEVKVLSTERSSSTALVTKSVSRILKGDQFQVKYQADLIPPPAPAKRSMGAPP
                     AEHSGDSISVQNASRETRYSLTDLMKQLRYESGEARITPEGFVTLGRLVDLLKAAPAD
                     QLIRVEGHADNMEIGPSLKSVYPTNWDLSKARATGVLRYLVEKGGIDSARVSSIGYGD
                     SKPIVSNATEAGRSKNRRVDIVLYSTETTTQAPSEPMRAGDDGYRVSGLGADEQRPAV
                     STVDHASSAEAAEPQKPVSVPAQATPALPAGESQALPAPTDAPVQP"
     gene            3246..>3938
                     /locus_tag="VFR82_04475"
     CDS             3246..>3938
                     /locus_tag="VFR82_04475"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500885.1"
                     /translation="MTGDARLSPPIEIETLYADVIVPRHLTRPFTYMVPAHLRPVLRI
                     GHVVSVPFGRSLIHGAVIAVTGNHPAGLDRQRLKSIRALITAGRASEISPTLLELARW
                     VAETYVAPYGQCLRLVLPPRSNTPDQSRILLTRKGQDALTTNGLPTQPELELLKRLKR
                     RPLGIPLSRLRVGKTRCQEEVLTAILAREWAHKVPGRPSSHASVPQPLMTSSSSDLWC
                     AEGDQHKDALQGM"
BASE COUNT          885 a         1173 c         1070 g          810 t
ORIGIN      
        1 ggattccggt acgcgggtct tgagacggat ccggtgccgc tttctgatcg aataaaattc
       61 atagacgacc tcgaaccgct cttcgtcgtc gggccagtcg acggagctga catgcacgat
      121 gtagtcaaag tccatggccg ggtcgtcgtg caaaaactga gcgacctcat gcaacttatc
      181 cctggtcact gtgatcgcca ggtcgccacg ccactcgacg gccttcacaa agccgacagc
      241 gaagtgttcc tgaaggcgtt cggccagttg ctgaagcgac ataggcctct aggctttcaa
      301 actcgctctg acttgctcag gctgcttgac gaacacgcgt ttttgcatga tgcgttcttg
      361 cagcttgagg atgccgtcaa acaaggcttc cggggtcggg ggacagccgg gaacgtaaat
      421 atctaccggc acgaaacggt cgacaccctg cacgacgctg taactgtcat aaatattgcc
      481 ggatgtcgcg cacgatccca tcgcaatcac gtacttcggt tcaggcattt ggtcgtatat
      541 ctttcgaata accggcgcca ttcgccggca gacggttcct gccacgatca tcaaatcgga
      601 ttgcctcggc gaggcccgga acacaccggc gccatagcga tccatgtcat agcgcgagga
      661 gacggcggcg atcatttcaa tggcgcagca agctaagccg aacgtcattg gccacagcga
      721 ccctttgcgc gcccagttca ctgccttttc aaccgacgtc gtgatgacgt caggcatgcc
      781 gtctttgtca tgccgtccca actggattag tcccattcca atgctccctt acgccaggcg
      841 tagacgtagg ccacgaggaa caacgcgata aaaatcagca tctcgaccag accgatgaca
      901 ctgaccttgt tgatgtcgtt aaagaccacc gcccagggat acaaaaagat gacctcaata
      961 tcgaaaatca cgaacaacat agcgaagata taatagcgaa ccgggaacgg cattctggca
     1021 tcagaaaacg gctcagatcc gcactcatac gcagacaact tttcaggttc cgggtacttg
     1081 ggttgaacca aatagcttag caacagggta acgacgccga acgtcagcgc aataaaaatg
     1141 aacagcagaa tcggaaaata tttggtcaga tattccagga gtaactcaag accgctcatg
     1201 gagttgacct ttcaacacct tgagttgcaa ggagattggg atattaaacg gggggttgcc
     1261 tgatagcaag cgcacaaagg acctacaggc gcaagaccct gaagtcctat cactcctgcc
     1321 gagtccgagc ggtcgccgaa cgcatgagtg ccgaatccgt ccggaggtgg attggggcgg
     1381 ggccatcttg tttgtcccat tccggaagga gaggggagaa ggccttcgcc ttcttccggc
     1441 gatgaatttg cgtctccaag cggggaggag ctgactgttc cggtttccgc ggcatcgcgt
     1501 gcctcgtcag agcccatcag gatccaaata cgtgaaaacc atagcaccgg cctctattca
     1561 cggtcaatgg gggcaagatg gcaatatcct tgactgtaca ggactatttg ctatggtctt
     1621 gtaagacgct tcgtcttaac agaaggacca cccatgacga ctctaccatc acgcaccttc
     1681 tttggctgct tactgctcgc ggttttcagc ctcacgggac tcccccacgc ggtggccgat
     1741 gagctagaca ctattcgtta cggtgaagcg gcacgcacct atgctgccgg atccattcaa
     1801 cagtcgactc cccaagacgg tgtggtcaat gtcatcaccg gtgacaatca aaccgtcggg
     1861 aatcgcatgc ggctcggaca gaacgatgtc ctctatttga agttgaagca tcccggtgag
     1921 gcggcagtcg gcgatctcta caccgtctat aagcgcaccc ggaaagtgtt tcaccctgcg
     1981 acaggccagt acttgggcta tttgatcacc cggcttgcaa tcgtgcaggt cgtccaagtg
     2041 gacaaggagt tgaccacggt ccgggccctg cgtgcgttcg gcgcagcagc ccccggtgat
     2101 ccagtcatga aattcgccct gccacattcc gatgaagctg ccgtcattcc ggcctcaagc
     2161 gatgtgacgg gcatggtcgt cgaactgcaa gccgatttac agatgacgct cgtagcacag
     2221 cgcaacattg tgtatctcga tcgcggatct gacgatgggc taaaaccggg agaccgcatg
     2281 gatctaatcc ggtatggcgg cgcgctgccg cctcggcagg tcggagaagt caaggtctta
     2341 tcgaccgaac ggagcagctc aactgcgttg gtcacaaaat cagtttctcg gattcttaag
     2401 ggcgaccagt tccaggtgaa gtaccaggcg gacctcattc ctccaccagc gccggcgaaa
     2461 cggtcaatgg gtgctcctcc ggccgaacac tcgggggatt ccatatccgt tcagaacgcc
     2521 tcacgcgaaa cacgctacag tctcaccgat ctcatgaagc aattgcgcta tgaatcagga
     2581 gaagcgagga tcacgcccga aggctttgtc acgctcggcc ggctggttga tctcttgaaa
     2641 gccgctccgg ccgatcagtt gatccgggtc gaaggacatg cggataacat ggaaatcggc
     2701 ccttcgctaa agtccgtgta tccgacgaat tgggacctct ccaaggcgcg tgccaccggt
     2761 gtcttgcgat acttggtgga aaaaggcggg attgattccg cccgagtgtc gtcaatcggc
     2821 tatggcgatt ccaaaccgat cgtgagcaat gccaccgaag cgggtcgttc gaaaaatcga
     2881 cgcgtcgata tcgttctgta ctctaccgaa acgaccactc aagctccttc ggagccgatg
     2941 cgcgcagggg acgacggata ccgtgtgtcc ggccttggcg ccgacgaaca gcggccggca
     3001 gtatcaaccg ttgaccacgc gtcatctgca gaagccgcgg aaccacaaaa accggtttct
     3061 gtacctgctc aggccactcc ggctcttccc gccggcgaga gccaggccct gcccgcgccg
     3121 accgacgcac cagtgcaacc gtaaacttca ttctccaggc tggagcatgc gagattccgc
     3181 atgctccagc aagatcccct ccggctcatt gttccacctt cccgcggtcg ctgctacaat
     3241 gccgcatgac cggtgacgcg cggctctctc cgccaattga gatcgaaacg ctctatgcgg
     3301 atgtgattgt cccgcgccac ctcacccgtc cgttcacata catggtgccc gctcatttgc
     3361 ggccggttct gcgcatcggg cacgtcgtct ctgtcccctt cggtcgatcg ctgattcacg
     3421 gggcggtcat tgcggtcaca ggcaaccatc cggcaggact ggaccgtcag cgtctgaagt
     3481 ccatacgtgc tctcatcacg gccggccgtg cctccgagat ttctccgacc ctgcttgaac
     3541 tggcccgatg ggtggctgag acctatgtcg ctccctacgg acagtgtctg aggctggttc
     3601 ttcctccacg ttcgaacacg cccgatcaaa gccgtattct tttgaccagg aaggggcaag
     3661 atgcccttac gacaaacgga ttgccgactc aacccgagct cgagttgctc aagcgtctca
     3721 agcgacggcc attgggcatc cccctgagcc gcctccgggt aggcaaaacc cgatgccagg
     3781 aggaagtgtt gaccgcgatc cttgcccgag aatgggctca caaggtccca ggccgcccat
     3841 ccagtcatgc ttctgtccca cagccactga tgacgtcgag cagctcggat ctctggtgtg
     3901 ccgagggtga tcaacacaag gatgccctgc aaggcatg
//
LOCUS       DASTQY010000138         7273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_505292, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000138 DASTQY010000000
VERSION     DASTQY010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505292"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VFR82_04480"
     CDS             <1..1365
                     /locus_tag="VFR82_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU4500886.1"
                     /translation="TKESRLTDIVVEQSKFDPTLSVNGQYNRTVNPLNRPVFGGTGGS
                     LNEITIFDQRNGSVTVDATTNLLTGGNLDLNYSPARTNVNQDVASGFLFNPAWTGGLA
                     LTLTQPLLRTAGIDINKTFIRIAQNNAMVEEHVFRDRVLTVVATVEQTYWEVVFANEN
                     LKVAEAALKAAQELLATNRAKAKAGVMSIVDVLQAEAAVASRVEQVLVAEKSIRDQED
                     LLRRLLNPGEDELRQDVRLTPKDQPVVALEPISLPEAIDLAIDLRPEIVQAKKNLESS
                     ELNTKFAKNQLLPTLSFQGTMGLAGLGKDYTDSVNRNFSGDFYNYGAGLVLSYPLGNR
                     SAWSTFNRRQIEGKNAEVSLVSIRQQIIIGVKEAVRRVQTDFKRIETTKSARIMAEKQ
                     LQAEQERLKVGLSTTRFVLDFQRDLATAQGNELRAIVDYNKSLSNLSRHKATTLERYS
                     LQVQ"
     gene            1372..2082
                     /locus_tag="VFR82_04485"
     CDS             1372..2082
                     /locus_tag="VFR82_04485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU4500887.1"
                     /translation="MAPPGRLGTSVQANERGALLALLPIAATIDYYALPDSLQEQVLI
                     QLAPQLISYLALLLWAAHNDFVLRRIGLERGLWNRGAKLGVVTGLLLGCLNSFVILRV
                     LPWLGWDITFLTHTPHARVPVMIMVPWFIAFIAIVVEINFRGFLLGRLAALESGLWPR
                     PALRHLSPAAMLTSALVFAFDPFMVNTFKQMHWIALWDGLVWAVLWIRTRNLYATIVA
                     HAIEVIIVYSAVRTVLMS"
     gene            2085..3104
                     /locus_tag="VFR82_04490"
     CDS             2085..3104
                     /locus_tag="VFR82_04490"
                     /inference="COORDINATES: protein motif:HMM:NF002558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500888.1"
                     /translation="MTPSFSSYLVNDCFGDPGVYVEVRWSKRALLFDLGQNDVLGPTR
                     LLRANDIFISHTHMDHFIGFDAVVRVALGRGQKLRLYGPAGLIDNVHGKLRGYTWNLV
                     DGYPLIIEVQEFDRTEVRQATFHAANGFVLSMPTIHPLVVHPDTGLFTVLEDPMFTVK
                     AVALNHRIPSFAYALEEQFHINVNKQKLHEAGLPVGGWLRDVKQHVWAGKPDDFRFTA
                     LLFDEHRQMEQEFVLGDVRNRFLTISRGQKLAYVVDARFDEANESRIVALAQHADLFY
                     CESPYLDVDAEKARDRYHLTARQAGVMARKAQVRDLIVFHFSPRYTGQGEAIEQEAHD
                     AFLGH"
     gene            3115..3453
                     /locus_tag="VFR82_04495"
     CDS             3115..3453
                     /locus_tag="VFR82_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 domain-containing protein"
                     /protein_id="HEU4500889.1"
                     /translation="MNELGKYAVYFLLGGTIVSVSTYLGAQGRPYLAAFASTFPAITG
                     ATFILLYVNGGSDAIVAYAEKLFWFVPPWIVYVLAMIFIVPRLGFWPAMTLSLALYMG
                     CVWLVRLAVR"
     gene            3644..4162
                     /locus_tag="VFR82_04500"
     CDS             3644..4162
                     /locus_tag="VFR82_04500"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4500890.1"
                     /translation="MISLVELVAICSVATLTAAGASQGWDRLRRPRVNRSTDREKSAG
                     DGSGRPSAGAWHEPRYDKRHSVRCPIEYQVGAHRQEGILIDMSRRGWRAAGPSPVLAG
                     TLLSVRVFFPDAAQSVYIEEAVVRWSDGLEFGVELTRISPEDAQVLSDYLSVHYPMDP
                     SPSYAFSPFSYN"
     gene            complement(4280..4858)
                     /locus_tag="VFR82_04505"
     CDS             complement(4280..4858)
                     /locus_tag="VFR82_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500891.1"
                     /translation="MTQPIVPTDYLGIGRQLARLLGSSLEGPSGEALKELARAYDPSA
                     NEARISAEVFLFHKYLIVQTCVGMFPSPEVERVIGGLFAALNERATGLEFSQERQNAM
                     ERMWQMRAKQFDQPFTQDREHFLDEGSDGVFWKRSLTQFCQNVLEVDHPPDIWAGGKG
                     PSHKASTCVTEAVDHLVAAMTEMSKLHFGSAA"
     gene            4971..>7273
                     /gene="uvrA"
                     /locus_tag="VFR82_04510"
     CDS             4971..>7273
                     /gene="uvrA"
                     /locus_tag="VFR82_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU4500892.1"
                     /translation="MPDTTAVLVVMAAVVRRAARCEWETALAGTSARLLGCRVMLRRE
                     FDVSYLPEAESASQALIVEGARQNNLKNISVLIPHNQVTAITGLSGSGKSSLAFDTLF
                     AEGQWRYVESLSTYARMFLDKVARPDVDRVLNVRPAIAIEQKNQVRTARSTVGTTTEI
                     ADLLRLLFAKIGKPICPDCHEEARSFQPDTVTNDLLERFADAKAMLLFPVTPPSAKAE
                     SAFVQSLLTRGFTRLRMGHETIDLHQDALPSSAHRLSSLSVVLDRLVIREDNRSRLCE
                     SIEMAFREGDGRCDVEVIGYGLQQYHAGFCCQRCGRTFEPLRPVLFSFNHPLGACPDC
                     KGFGNVLRYDPALVIPDHSKSLVQGAIEPWSKPGTDWWQKQMLLAMKRKGVDITRPFK
                     LLEEDVKKLLWKGDKSFDGIDDFFQYLEGKRYKLHVRVLLSRYRTPFDCPGCHGSRLK
                     PHARFVKIGGVDIHDITGMTIEHLMAWLQGLALRPYERGIAADLLRQLTAKLGFLIRV
                     GLGYLTLGRQTKTLSGGEAQRVSLANQLGARLVGTLYVLDEPTIGLHARDTDLLAGIL
                     KDLAAAGNTVVVVEHDRQMIESADHIVELGPQSGEKGGEIVCAAPAGDFLRDRRAITA
                     RYLRGEDHIPLPASRRSGTGKVLVIAGASEHNLKDLVVRFPLGMLICVTGVSGSGKST
                     LVEDTLYAALARAFRTETVPMGRFKAIRGIEHLKGVRLIDQQPIGRTPRSNPVTYLKA
                     FDEIRRLFAGEREALRQGFAPGHFSFNA"
BASE COUNT         1503 a         2118 c         2102 g         1550 t
ORIGIN      
        1 acgaaggaga gccgcctgac cgacatcgtt gtcgaacagt cgaaattcga ccctacgctc
       61 agcgtcaacg gacaatacaa ccggaccgtc aacccgctca accggcccgt cttcggcgga
      121 accggtggaa gtctgaacga gattacgatc ttcgatcaac gcaacggctc tgtgaccgtg
      181 gacgccacca cgaatctctt gaccggcggc aacctcgatc tgaattacag tcctgcccgt
      241 acgaacgtca accaggacgt ggcgagcggc ttcttgttca atccggcctg gaccggcggt
      301 ctcgcgctca cgctcacgca gccgttgctg cgcactgccg ggatcgacat caacaagacc
      361 ttcatccgga tcgcccaaaa caacgccatg gtcgaagagc atgtgtttcg cgaccgtgtg
      421 ctgactgtcg tcgcgaccgt cgagcagaca tattgggaag tcgtctttgc caacgagaac
      481 ctaaaagtgg cggaggcggc attgaaagcc gcacaggaat tgctcgctac caacagggcg
      541 aaggccaagg cgggggtgat gtcgatcgtg gacgtgctcc aggcagaggc ggctgtcgcg
      601 tcacgtgtcg aacaagtgtt ggtcgccgaa aagtccatcc gcgatcagga agacctgttg
      661 cgtcggctgc tgaacccggg ggaggacgag ttgcgccaag acgtgcgatt gaccccgaag
      721 gaccagccgg ttgtcgctct ggagccgatc agtctgcccg aagccatcga tctggccatc
      781 gaccttcgcc cggagatcgt ccaggcgaag aaaaacctgg agagcagcga actgaatacc
      841 aagtttgcca agaaccaact gcttcctacc ctctcgttcc agggcacgat gggtctggcc
      901 ggactcggaa aggactatac cgattctgtc aatcgcaatt tcagcggaga cttttacaac
      961 tatggcgcgg ggctcgtgct gagctatccc ctcggcaacc gctcggcatg gagcacgttc
     1021 aacagacggc agatcgaggg caagaacgcg gaggtgtcgt tggtgagcat caggcaacag
     1081 atcatcatcg gcgtgaaaga agccgtccgc cgggtccaga ccgatttcaa acgcatcgaa
     1141 acgaccaagt cggcgcgcat tatggcggag aagcagctgc aagccgagca agaacgtctg
     1201 aaagtcgggc ttagtaccac gcgtttcgtg ctcgatttcc agcgggacct tgcgaccgct
     1261 cagggcaatg aattgcgtgc cattgttgat tacaacaaat cattgtccaa tctgtcccgc
     1321 cataaagcca ccacgctgga gcgctatagc ttacaggtcc aatagcgacc gatggcgccg
     1381 cctggtcgtc tcggcacctc cgttcaggca aacgagcggg gtgcactgtt ggcactcctg
     1441 ccgattgccg caaccatcga ttactatgcc ctgcccgact cgctccaaga acaagtcctg
     1501 atccaactcg ctcctcaact catcagctac ctcgccctcc tcctgtgggc agcgcacaat
     1561 gatttcgtcc taagacgaat cgggttggag agggggctgt ggaaccgcgg agcgaaactc
     1621 ggcgtcgtga ccgggctgct cctcggctgc ctcaattcct ttgtgatcct tcgggtcctg
     1681 ccctggttgg gatgggacat cacgttcttg acgcacacgc ctcacgcccg cgtaccggtg
     1741 atgatcatgg tgccctggtt cattgcgttt atcgcgatcg tcgtggagat caacttcagg
     1801 ggttttctgt taggccggct tgcggcgctc gaatccggcc tctggccgcg acctgctctc
     1861 aggcatcttt cgcccgcggc catgctgacc agcgcattgg tcttcgcctt cgatccgttc
     1921 atggtcaaca ccttcaaaca gatgcactgg atcgcgctct gggacgggct cgtatgggca
     1981 gttctctgga ttcggacgag aaacctgtat gcaacgatcg tcgcgcatgc gattgaagtc
     2041 atcatagtat atagtgcagt gcgaaccgtg ctgatgtcgt agtcatgaca ccttcatttt
     2101 ccagctacct ggtgaacgac tgcttcggag atccaggcgt ctacgtggag gtccgatggt
     2161 cgaagcgggc gctgctattc gatttagggc agaacgacgt gctcggcccc acccgcctgc
     2221 tgcgcgcgaa cgacattttc atatctcata cgcacatgga ccacttcatc ggattcgacg
     2281 ccgtggtccg tgtcgcgctg ggtcgtgggc agaagctccg cctgtatggc cccgccggct
     2341 tgatcgacaa cgtgcatggc aagctgcgcg ggtacacctg gaatctggtg gatggctatc
     2401 cgctgattat cgaggttcag gaatttgacc gcacggaggt tcggcaggcg acgtttcacg
     2461 ccgcaaacgg cttcgtgctc tcgatgccga cgattcatcc gctcgtggtt catccggaca
     2521 cgggcctctt tactgttctc gaagatccga tgtttaccgt taaggcggtc gccttgaacc
     2581 atcgcatccc ctccttcgcc tatgcgctgg aggaacagtt ccacatcaac gtgaacaagc
     2641 aaaagttgca cgaagcggga ttgccggtgg gcggctggct aagagatgtc aagcagcatg
     2701 tgtgggcagg aaagccggat gacttccggt tcaccgctct cctgttcgac gaacaccgcc
     2761 aaatggaaca ggagtttgtg ctgggtgacg tcagaaaccg cttcctgacc atctcgcgcg
     2821 ggcaaaaact ggcgtatgtt gtcgacgcgc gtttcgacga ggccaatgag tccaggatcg
     2881 tcgcactcgc gcagcatgcg gacctgttct attgtgagtc accgtatctg gatgtcgatg
     2941 cggaaaaggc gagagaccgt tatcacttga cggcaagaca ggcgggcgtc atggcgcgca
     3001 aggcgcaagt gcgggacctc atcgtgtttc atttctcacc acgatatacg ggccaaggtg
     3061 aggccatcga acaagaagct cacgacgcat ttctaggtca ctgaggagcg atacgtgaac
     3121 gaattaggaa aatatgccgt gtacttccta ttgggaggca ccattgtcag tgtctcgacc
     3181 tatttgggcg ctcagggcag gccgtacctg gccgcctttg cgagcacctt tccggccatc
     3241 accggcgcca ccttcattct gctctacgtc aatggcggca gcgatgccat cgtcgcctat
     3301 gcggagaagc tgttctggtt cgtcccgccc tggatcgtct atgttctggc catgatcttc
     3361 atcgtcccgc ggctcggctt ctggccagcg atgacccttt ccttggcact ctacatggga
     3421 tgcgtctggc tggtcagact ggccgtgcgc tgaaccctcc ctccgaagta gggattcgat
     3481 caagacgatc aagatggaga acgtaccgca aggaagggga ttctcgcgaa taccccccca
     3541 taggtctacc ctctttgaat actaccggac agaccctgcc ggcgatagag tcactacatg
     3601 accttcgaac aacttccaga aggccgtcgg gtcgggaggc gccatgatca gccttgttga
     3661 gcttgtcgcg atttgtagtg tggcgacctt gaccgctgcc ggtgcatccc aaggttggga
     3721 ccgcttgcgg cgaccgagag tcaaccggtc taccgaccgt gaaaaatccg ccggcgatgg
     3781 atcaggacgc ccatccgctg gggcatggca cgaacctcgc tatgacaagc ggcactcagt
     3841 caggtgccct atcgagtacc aggtgggagc acacaggcaa gagggcatac tgatcgacat
     3901 gtcgcgtcgg ggttggcggg cagcgggtcc ttcgccggtt ctcgctggca ccctcctatc
     3961 cgtgcgggtc tttttcccgg acgccgctca gtcggtctac atcgaggaag cagtggtgcg
     4021 gtggtccgat ggcctggagt tcggagtcga attgacgcgt atcagccctg aggacgcgca
     4081 agtgctcagc gactatctgt ccgttcacta tccgatggat ccttcccctt cgtacgcctt
     4141 ctcgccattt tcctacaact aagctgagct catgtgttcg attcccgaga actcagctca
     4201 tcgatcaggg atgctgtccg ttgattcgca cgatgtgcgg ttccgattag gcgtggggag
     4261 agaagtagag tcaggcaagt caggctgcgc taccgaaatg cagtttactc atctccgtca
     4321 tggctgcgac gagatgatca accgcttcgg tgacacaagt actggccttg tgggatggac
     4381 ctttcccacc ggcccagata tccggcggat gatcgacctc aagcacattt tgacaaaatt
     4441 gggtgaggga ccgcttccaa aacactccgt cgctgccctc atcgaggaaa tgttcgcgat
     4501 cctgtgtaaa gggctggtca aactgcttgg cccgcatctg ccacatccgt tccatggcat
     4561 tctgccgttc ctgactgaat tcgagaccgg tcgcacgctc gttcaacgcg gcaaacaggc
     4621 cgccaatcac ccgctcgact tcggggctgg gaaacattcc cacgcaggtt tgcacgatca
     4681 agtatttgtg gaagagaaac acttccgcgc tgattcgtgc ctcattggct gacggatcat
     4741 aggcgcgcgc gagttccttc aatgcctctc cggaaggtcc ttccaagctc gaacccagca
     4801 atctcgcaag ctggcgtccg attcctagat agtcggtcgg cacgatcggc tgggtcatac
     4861 aagagtgatt cctttaaggg cgatggtgag cttagaggat tgcatttgag cctgtccatc
     4921 ctctcactca ccccaagaat ccccaagaca aaactacaat tcgcgccgtg atgccggaca
     4981 cgacggctgt cctcgtcgtt atggctgcgg tcgttcgccg agcggcccgc tgtgagtggg
     5041 agacagcgct ggcaggaacc agcgcgcgcc tgctaggatg ccgggtcatg ctccgaaggg
     5101 aattcgacgt gtcttacctt ccagaagcag aatcggcgtc acaggcgctc atcgttgaag
     5161 gggcacgaca aaacaacctg aaaaatatct ccgtcctcat cccgcataat caggtcaccg
     5221 ccatcaccgg cctctccggt tccgggaaat cctcgctggc gttcgatacg ttgtttgctg
     5281 aaggccaatg gcgctatgtg gagtcgctct ccacgtatgc caggatgttt ctggacaagg
     5341 ttgcccgccc cgatgtcgat cgtgttctga acgtccgtcc ggccattgcc attgaacaaa
     5401 aaaatcaggt gcggaccgcc cgttcgaccg tggggacgac taccgaaatc gcggacttgc
     5461 ttcgcctgct ctttgcaaaa attggaaagc cgatttgtcc ggactgtcat gaagaggctc
     5521 ggtcgtttca accggatacc gtcacgaacg atttgctcga gcggtttgct gacgctaagg
     5581 ccatgctgtt attcccggtg acgcctcctt ccgcaaaggc ggaatcggct tttgtgcaat
     5641 ccctccttac ccgtgggttt accagactga ggatgggtca tgaaaccatt gaccttcatc
     5701 aggatgcgct cccctcctca gcgcaccgcc tgtcgtccct gtccgtcgtc ctagaccgac
     5761 tcgtgattcg agaagacaac cggagccgtc tttgtgagtc gattgaaatg gcgtttcgtg
     5821 aaggagacgg ccgctgtgac gtggaggtca tcggctacgg actgcagcag taccatgcag
     5881 gcttttgctg ccagcgatgc ggacgcacct tcgaaccgct cagaccggtc ctcttttcgt
     5941 tcaatcaccc gcttggcgcg tgcccggact gtaaagggtt cggcaacgtg ctgcgctacg
     6001 atcccgcgct ggttattccc gatcacagca aatcgttggt gcaaggcgcc atcgagccat
     6061 ggagcaaacc gggaaccgac tggtggcaaa aacagatgct cctcgcgatg aagcgaaaag
     6121 gcgtggacat cacacgccct ttcaagttgc tggaggaaga cgtcaaaaag ctgttgtgga
     6181 aaggcgataa gtcattcgac ggaatcgacg acttctttca gtatctggag ggcaaacgtt
     6241 acaaacttca tgtgcgcgtt ctgctcagcc gctacaggac gccgttcgat tgtcccggct
     6301 gtcacgggag ccggctcaag cctcatgctc gtttcgtcaa aatcggcggg gtcgacatcc
     6361 atgacattac cggaatgacc atcgaacacc tgatggcgtg gctgcaaggc ctggccctac
     6421 ggccgtatga gcgagggatt gctgccgatc tcctacggca gctcaccgcg aagctgggct
     6481 ttctcatccg ggtaggtctc ggctatctga ctttagggcg gcagacgaaa acgctgtcgg
     6541 gaggcgaagc ccagcgtgtg tcgttagcga atcaattggg tgcacgcttg gtcgggacac
     6601 tgtatgtact cgatgaaccg acgattggcc tccatgcccg cgatacggat ttgctggcgg
     6661 ggattctgaa agacctggcc gcggccggca acacggtggt cgtcgtcgaa catgaccggc
     6721 agatgatcga atcggcggac catattgtcg agctggggcc gcaatccggt gagaagggcg
     6781 gtgaaatcgt ctgcgccgcg ccggccggcg actttctccg tgatcgccgt gcgattaccg
     6841 cccgctatct caggggagag gatcacattc ccctccctgc ctccaggcgc tcagggacgg
     6901 ggaaagtcct tgtgattgcc ggcgcgagcg agcacaacct gaaagacctg gtcgtgcggt
     6961 ttcccctggg tatgttgatc tgcgtcacag gggtctccgg ctccgggaag agcacgctcg
     7021 tggaagacac gctgtatgcc gcattggcgc gcgcgtttcg gacggaaacc gtgccaatgg
     7081 ggcgcttcaa ggcgatcaga ggaatcgaac atttgaaagg cgttcgattg atcgaccagc
     7141 aaccgattgg ccggacaccc cgatccaatc cggtgaccta tctgaaagcc ttcgacgaaa
     7201 tcaggcgtct atttgccggg gagcgcgagg cgctacggca gggtttcgcg cctggacatt
     7261 tctctttcaa cgc
//
LOCUS       DASTQY010000139         6229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1393167, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000139 DASTQY010000000
VERSION     DASTQY010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393167"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(434..949)
                     /locus_tag="VFR82_04515"
     CDS             complement(434..949)
                     /locus_tag="VFR82_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU4500893.1"
                     /translation="MLERLLGAFPPLIQMLRDDHKAVRTLFTRYDKAKNGEKPQIAQH
                     IIKELTVHAKIEERVVYPAFRAVFPSPQLVYEAIEEHHLLHVLVEELSRFRPGPGTAT
                     FEAKFKLLRELVKHHVDEEEGRVFPKAEAQKLDWPLLYEKAQRIKKQQQQSVGKKKRP
                     TAPKPSHKRAA"
     gene            complement(2217..4424)
                     /locus_tag="VFR82_04520"
     CDS             complement(2217..4424)
                     /locus_tag="VFR82_04520"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4500894.1"
                     /translation="MVAASAYFINLHAIPMFLMGIVTLTLGLLVYRSNNQSAAHRHFL
                     VLCASIALWLDATSIALCSTDADTALRWFKLDNVGVMYISVAFYGFSAEFLQLRRRRS
                     ILIGYVLATLMAGAVLFRQDFVQGVHRFWWGYFPQWGPASLPFFSIFFSYMAVAFTDY
                     FRTYRTVTTPIKRQQIKFVLIAFVIAYLGSVDFLPAFGYELYPFGYIPIFLLTAVITV
                     AILRYRLLDAALFVSRSAVYLPLIPFTLLIVFLGDALDDLSPDTLSLLLALTTVVFTI
                     LYVTFQPRLQSAVNKALFPSRHDAYETLTRFAHAMVMKLDLVNLQAEIVDTLQAVMGI
                     ERISLFVFDKEHGSYVLKASRGFTENKTNRGQVSNNTIFSNYLLESNQPIVKEELQQR
                     PPGLDERLATTLIETMTQMDSEVCLPLVNKTRLIGFVNLGHKPSLGFYSHDELNLLRS
                     LADNAAIALENAVLHEDWKQTQLLLRRADRLRSLEAIAGGFAHEVRNPLTSISTFIQL
                     VPSRRDDSYFMDSFSAQVVDDLARIERLIAEILDYARYMKPKFSAESLNNIVASCLQF
                     IEVKADKLEISIEKHLAEDLPPMLVDRQQIKQVMMNLAMNAMDAMAKTGGRLTVTTRR
                     VVRLDKSGWVQIEVADTGCGITPEDLPHVFDPFFTTKHESTEHVGTGLGLSIVHQIIQ
                     EHGGTVEARSTVGKGTSFLITLPEGRSLDPLAAPASSEWQGGVITARRIPTLQ"
     gene            complement(4431..5960)
                     /locus_tag="VFR82_04525"
     CDS             complement(4431..5960)
                     /locus_tag="VFR82_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500895.1"
                     /translation="MPLPGLVAAVTELEGDSPTRFRIDIALADLGHTERLVFESALQE
                     LETYLQSLEMPESTARAAARATLVTANPRSILSLFITDNAYQLTYGSPAGHDKRSQSP
                     APPVSPRSFRETFEKTEHTPMRVAWKVVWQGVGLFLHLIRDLIVGWLPGRLAKLLITP
                     ITFAFIGHPRTLSDVPRKFPFASLLPLHVVERWFRYQWPFVASYITGLTLVDGTPTTG
                     AMLISPLTTEQMIRNPRLARERVLQTVRLAEKMGAKLAGLGAFTSIITRDGQELKEKV
                     RLGLTTGNAQSAAIAVQNVLHAAALTNLSLPHAIVAIVGGAGSVGSACAKILARVVGT
                     LLIIDIKRDELQTLLAQLGDQPARVKGMTTLDEVRQADVVIATTNNPYILLTADHLKG
                     GAIIIDAAQPKNVSEDVPLRRPDVLVIESAVVRTPNIDVHFDLDLGVREALGCLSETM
                     ILTAIGWNGHYSLGKADISQTAHITTVGRSLGFGLAPFRNSTGYVTEEDLHRVARARR
                     M"
BASE COUNT         1503 a         1618 c         1730 g         1378 t
ORIGIN      
        1 agttcattgt tgcacctctt gttaggttac ccgaggtgta cgccgaatgg gcggaggagt
       61 cgacgggtga aacccacggt ttaagacccc attggcgatt ggtccctcat tacatggcga
      121 ggggggtcca tctgatgtga gatagattgg aagccgatta ccaccagatg atgtgagttc
      181 gtgagatcca aggccctagg agcattccca gagcgtgggt accccacacc tcgcgggaat
      241 gcctgtacct ctgccgacta acacgagtcg gatgcgccct atcctttaga tgccaaaaac
      301 tggcaatcct gaagggccat ggatcgggca gttgacgggt gccgtttgca gccttatgtg
      361 gcccacgaat gctgctcgaa gtgttggtgg acgtgctgag gattcaatca gtgcaggcgc
      421 gctgtataga caatcaggca gcgcgtttgt gggaaggctt cggagcagtc ggccgtttct
      481 tttttcctac cgactgctgt tgctgttttt taattctctg ggccttctca taaaggagcg
      541 gccaatccaa cttctgggcc tccgcttttg gaaagacgcg cccctcctct tcatccacgt
      601 ggtgttttac caactctcgt agaagcttga acttggcctc gaacgttgcc gttccgggcc
      661 ccggacgaaa tcggctcaac tcctcaacca atacatgcag cagatggtgc tcttctatcg
      721 cctcgtacac gagctgagga cttggaaaca ccgctcgaaa cgccggatag accacccgtt
      781 cttctatctt ggcgtgcacc gtcaattctt tgataatgtg ctgagcgatc tgaggctttt
      841 ccccattctt tgccttgtca taccgtgtga acagtgtccg cacggccttg tggtcatcgc
      901 gaagcatctg aatgagtggt ggaaaggcac ctaacagtcg ttcaagcatt tgcagccccc
      961 cattgattgt ccaagcatat cgagccaacc atttaagtca cctaggcaga aacccaggtg
     1021 acctcgccgg gatttgcagc cggtcactgg ctaatctacc aattagttga ctaccctgca
     1081 cgcacccaca ctaagggtgg aggtttaggt gtgtgtataa gccagagccg accccggcta
     1141 gcctttaacg cgactgactc agcggtaaga gcagatattt ctttgctcca gtgatccatg
     1201 aggatcgccg gatgactctc gtctcagtga catcttgtcc caaacggtgg acaagacatc
     1261 cacgtaaacg acctcccgta gggaatcgca tggttcttcc ggactttttt gaaagcaagc
     1321 gtgggcataa ggcttgcctg ataacgaacg agatttccgc ttcaacagaa acggatcaag
     1381 agaggcagca tcatggcctt ttcaccgacc gacccggtgt ccgcacactc cctctggctc
     1441 cagttacgtt ccaccaagcc caaaaagctt cgtcgccctc gatggcgccg ggattgggcg
     1501 cccctttggg ttcctgtcct cttgcttatc attcttgcca tcttttacgt ggttatgccg
     1561 tccaatgacg ctgtgaaaag tgcagaagcc gttcttcaag agcatcctat gcttggtgca
     1621 atggcgggac aataagtgta tccagaaagc tacggactcg tcgatgctaa cgaggcgaga
     1681 acgagaaaga ccgcatccaa ttacggaggg agacttcgtt tcagccagtg aacgtctgta
     1741 ctttcttcgg ggatctcagg caaacgctca ccctcgcttg ctgcaatacc gcatacacga
     1801 tccagagatt gaagcctttt ttcacctaga gccaaggatg gccgcaattg gtgtgatgat
     1861 ttcgttttgc acgctctggc tcctcaccgt cctcggcctc tatcaaatca ctcgaatcgc
     1921 tttgcaagcc acaatatgac ttagatcacc gcagaggagg aagttcatgc agaagcaaga
     1981 agggaagatc ggagccattc gtagaaacgt gcatggcaag gcctgtgttt cctgtggaag
     2041 ccgtagctat caattggtac ttcaaagcgc gaacgaacga cacgttggca agctgttcgc
     2101 tcgctgtacg cagtgtcact gtaccagaga tataggtgaa gacctcgaac gaatactgtg
     2161 gatgtaacca acaaagtcca ttggtgtgat taaaatgggt tgagccaata gtcgcgttat
     2221 tggagagtag ggatacgtcg agcggtaatg accccgcctt gccattcgga cgaagcaggt
     2281 gctgccagcg ggtcgagact ccttccttca ggaagcgtga tcagaaagct ggtcccctta
     2341 ccgacggtgc ttcgagcttc caccgtgcca ccatgttctt gaatgatttg atgaacaatc
     2401 gagagaccga gccctgtccc cacatgctcg gtgctttcat gtttagttgt aaaaaaggga
     2461 tcgaagacgt gcggtagatc ctcaggggta atcccacaac cggtgtccgc gacctcgatt
     2521 tgcacccatc cgctcttgtc aagacgaacc acgcgacgag tcgtcacagt caaacggccc
     2581 ccggtcttcg ccatcgcgtc catagcattc atggcaagat tcatcatcac ctgctttatt
     2641 tgctgacgat cgaccagcat aggagggaga tcctcggcga ggtgcttctc gatagaaatt
     2701 tccagcttgt ctgctttgac ctcaataaat tgcagacacg aggccacaat gttgttcaag
     2761 gattccgcag aaaacttagg cttcatgtag cgtgcgtagt cgagaatctc tgcgatgaga
     2821 cgttctatac gggccagatc gtctaccacc tgagcactga acgagtccat gaaatacgaa
     2881 tcgtctcggc gggacggcac cagttggata aacgtactga tcgatgtcaa ggggttgcgc
     2941 acttcatgag caaatccacc tgcaatggct tccagtgacc gaagccgatc ggcgcgtcgg
     3001 agcaggagtt gggtctgctt ccagtcttcg tggaggacgg cattctcgag cgcgattgcc
     3061 gcattatctg caagagaccg gaggagattg agctcgtcat gcgaatagaa tccgaggctc
     3121 ggcttatgtc cgagatttac aaagcctatc aggcgtgttt tattgacaag gggcaagcag
     3181 acctcagagt ccatctgagt catggtttcg atcagggtgg tggccaatcg ttcgtcgagt
     3241 cctggtgggc gttgctgtaa ctcttccttg acgatcggct ggttgctctc cagcaaataa
     3301 ttcgaaaaga tggtgttgtt cgagacctgc ccgcggttgg tcttattttc agtaaatcct
     3361 cgcgacgctt tcagaacata ggatccatgt tccttgtcga aaacaaagag cgagattcgc
     3421 tcaattccca tgacagcctg aagcgtgtcg acaatctccg cctgtaaatt gacaagatcg
     3481 agtttcatga ccatggcatg tgcgaagcga gtcaatgttt cgtatgcgtc atgccggctg
     3541 ggaaacaacg ccttattgac ggcggattga aggcgcggct ggaaggtgac gtacaagatc
     3601 gtaaagacga ccgttgtgag tgcgagcagt aacgacaggg tatctggcga gagatcatcc
     3661 aatgcgtcgc ccaggaagac aatgaggagc gtaaatggga tgaggggtag ataaacggca
     3721 ctacgagaga caaacagggc tgcatccagg agccggtacc tcagaatcgc gacggtaatg
     3781 acggcggtca ggaggaaaat gggaatgtac ccgaagggat agagttcata accgaaggcg
     3841 gggagaaaat caacggaacc caggtaggcg atcacaaacg cgatgagcac gaatttgatc
     3901 tgctggcgct taataggagt cgtgacggta cgatacgtgc ggaaatagtc ggtgaatgcg
     3961 acagccatgt aggagaagaa aatagaaaag aaaggcagac tcgccggccc ccattgcggg
     4021 aagtatcccc accagaatcg atgaacacct tggacgaagt cttggcggaa caggacggcc
     4081 ccagccatga gcgtcgcgag tacgtatccg atcaagatcg aacggcgccg tcgaagttga
     4141 agaaattctg cagagaatcc atagaacgcg acggagatgt acatgacgcc cacattgtcc
     4201 agtttgaacc agcgcaacgc cgtgtcggca tcagtcgagc agagagcgat gctggtggca
     4261 tcgagccaaa gggctatgct ggcacagagg acgagaaaat ggcgatgtgc agccgattgg
     4321 ttattagatc ggtaaacgag aagacccaac gtcagcgtaa cgattcccat cagaaacatg
     4381 ggaatcgcgt ggaggttgat gaaataggcc gaagccgcga ccatggtgcc ttacatcctg
     4441 cgcgcccgag cgacccggtg tagatcctcc tcagtgacgt atccggtaga attgcgaaag
     4501 ggcgcgaggc cgaatcctaa agagcgacca accgtggtga tatgagcggt ttgggaaata
     4561 tcagctttcc caagcgagta gtgcccgttc cacccgatcg cagtcagtat catggtttcc
     4621 gataggcagc ccagcgcttc cctcacgccc aagtccagat cgaagtgcac atcaatatta
     4681 ggcgttcgga cgacagccga ttcgatgacc agaacgtcag gccgccgtag aggaacgtcc
     4741 tcggacacgt ttttaggctg tgcggcatcg atgataatgg caccaccttt caggtgatcg
     4801 gcggtgagaa ggatgtatgg gttatttgtc gtagcgatca cgacatcggc ctgccggact
     4861 tcgtcgagag tggtcatgcc cttaacccgt gcgggctggt cacccagttg tgcgaggagt
     4921 gtctgcaatt catccctctt tatgtcgata atcaagagcg ttccgacaac ccgagcaagg
     4981 atcttcgcgc acgcagaccc gaccgatcca gcacctccaa caatggccac aatggcatgc
     5041 gggagactga gattcgttaa agctgcggca tgaagcacgt tctggacggc gatggcggcc
     5101 gattgggcat tgcccgtggt cagtccgagc cggacttttt ccttcagttc ttgtccatcc
     5161 cgagtgatga tcgatgtgaa ggcgcctaac cctgcaagtt tggcccccat tttttccgcc
     5221 agacgaacgg tctgtaacac gcgctctcga gccaggcggg gattgcgtat catttgttct
     5281 gttgtcagcg gcgaaatgag catggcacca gtggttggtg tcccatccac caacgtcaaa
     5341 ccggtgatat aggaagcgac aaagggccat tgataacgga accatcgctc gacgacatgc
     5401 aaaggcagga gactggcgaa tgggaacttc cgtggaacat cactcagcgt gcgcggatga
     5461 ccaatgaagg caaacgtgat gggcgtgatc agaagtttgg ccaaacgacc aggtaaccat
     5521 ccgacaatca gatcgcgaat gaggtgcaag aagagcccga ctccttgcca cacgaccttc
     5581 caggcgacgc gcatcggagt gtgctctgtc ttctcgaatg tctctcggaa ggaccgaggg
     5641 gagacaggcg gagccggact ctgtgacctt ttgtcatgac cggccggtga cccgtaggtt
     5701 agttgataag cattatctgt aatgaacagg gagaggatcg accgaggatt ggcagtcacg
     5761 agtgttgcac gtgccgctgc acgtgcggtg ctctcaggca tctccaggct ttggagatag
     5821 gtttcgagct cttgcaaggc agactcaaaa accagccttt cggtgtgtcc caagtcggcg
     5881 agcgcaatgt cgatccggaa tcgggtcgga gaatcaccct ccagctcggt caccgccgcc
     5941 acgagtcccg gcaagggcat gtaagccaca ttgtggctga agcacaggac gacggtgact
     6001 gcagtgcctc gtgggcaaac acagtcggtc aagaatgaaa tagtgccaag gccgaggtct
     6061 tgagtgactc ctcgagcctg ccttccatcg aaatcgacca cgacgggcag gctgactgcg
     6121 tgctggaccg aaagggtact ctccagggcg ttattcctcc aggttaatgg ctctaaccct
     6181 tgacagaagc cgcttgccaa ggactctact catttttgcc gcaaaaact
//
LOCUS       DASTQY010000140         3343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_448739, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000140 DASTQY010000000
VERSION     DASTQY010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448739"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..551
                     /locus_tag="VFR82_04530"
     CDS             129..551
                     /locus_tag="VFR82_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEU4500896.1"
                     /translation="MTVKTMPLRIDMSGGTQIENVTKFVQEALAHAKVTAGIVTVFVQ
                     HTTASVMVIEDEPGIRLDTKTFWDRIAPANQSWQHNEQNPGEDNGHSHLRGQLQGPSV
                     TIPFSNGSLLLGTWQQIVVVDFDTRARTRELIIQILGE"
     gene            569..1684
                     /locus_tag="VFR82_04535"
     CDS             569..1684
                     /locus_tag="VFR82_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU4500897.1"
                     /translation="MAVLLAVMLQLAGEGRWCDVAAADWGKPLGALYEGVEVRPRPVT
                     PAPTELGPDERATMAVFERATRSVVFISNTAIQRDFWSFDILEVPQGSGSGFVWNKQG
                     HIVTNFHVIYGANTIKVTLADRSEHQAKLVGADPDHDLAVLQIQAPESLLEPLAIGSS
                     HDLRVGQKVLAIGNPFGLDHSLTTGVVSALGRTIKSLSNRTIEGVVQTDAAINPGNSG
                     GPLLDSAGRLIGVNTQIISPSGAYAGVGFAVPVDTVNRIVPELIKHGKLIRPGLGISL
                     VPDAIVRRWGIKGLVIGKVTQGGAADRAGLRSARETPTGRIELGDIVVAVAGKPVTTI
                     DELMAVLEDHKVGEQVIVEILRGNRREKITVTLQAVN"
     gene            1744..>3343
                     /locus_tag="VFR82_04540"
     CDS             1744..>3343
                     /locus_tag="VFR82_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4500898.1"
                     /translation="MSDRRSADSPQEPTSRREDPGTKTGSDPLELIDQCLALFPESDP
                     RQKLLYKLRHVVMLSQAANQQREAEFRKVSEVVAKLTAPANRIGMLLALPGEDLAHIL
                     VGGAEYYANVDPRVATGDLKIGTQILVNEAYAVIKTLGYDRNGPILKLTEAMADGRLR
                     FEQEMGRQSLILQRSSDLVGVELKAGDEVRIDTSLRIAIEKLADRKAAKHVLDEAPNV
                     TWEQIGGQQEAIAAIRKAIEYPLLHASTFEQFKFSQPKGFLLYGPPGCGKTLIGQAAA
                     ASLSKLVSESGPDVHGGRDTRAPITGGAFLHVKGPEILNMWLGESERMVRDLFAQARA
                     KRHEGALPFIFIDEAESILGTRRAMRSFNISNTLVPMFCSEMDGIESLRDVVIILASN
                     RPDLIDPAVLRPGRIDRKIKVARPNREASAQILKVYLRHDLPLDPALLAERGGEQGRV
                     MASLADDVLEQVFKRSDENRLLSVRLRNGQQKVLYRGDLVSGAILASIVQRAKEKAID
                     RTIQSGQPAGLLSQDLFDAVTEEFR"
BASE COUNT          715 a          952 c         1027 g          649 t
ORIGIN      
        1 gtggatgtga tgaaggtcat gaaacggctg gccgcctccg gggagttcga cggcgtgatt
       61 gcggcagcac gaggtcgtcg catgacgtcc gatgagcgac gattccttcg tcgaatggag
      121 cggtgagtat gacggtcaaa acgatgcctc tcaggattga catgagcggc gggacgcaga
      181 tcgagaatgt gacgaagttc gtccaggagg cgttggctca tgccaaggtg actgccggca
      241 tcgtaaccgt gttcgtccag cacacaaccg cgtcggtcat ggtcatcgaa gacgagcctg
      301 gcatcaggtt ggatacgaaa accttttggg accgaatcgc tccggctaat cagtcttggc
      361 agcacaatga gcaaaatcca ggtgaagaca acggccatag tcatctgaga ggccagctgc
      421 aaggtccttc cgtcaccatt cccttttcga acgggtcact gctattgggg acatggcaac
      481 agatcgtcgt agtggatttc gacacccgcg cgcgcactcg agagctcatc attcaaatcc
      541 tcggagagta gcctcgatgg ttcctgtcat ggcggttctg ctggccgtga tgctccaatt
      601 ggctggcgaa gggcgttggt gtgatgttgc ggctgccgat tggggaaaac cgctcggtgc
      661 gctctatgag ggtgtcgagg tgcgaccgcg gccggtcacc ccggctccca ctgagctggg
      721 gcctgatgaa cgggccacga tggcggtgtt cgagcgagcg accagatcgg tcgtgtttat
      781 ctcgaatacc gccatccagc gggatttttg gtccttcgac attctggagg tccctcaagg
      841 atccggctca ggcttcgtgt ggaataagca gggacatatt gtcacgaatt ttcatgtgat
      901 ctatggggcg aacacgatca aggttacgct ggcagaccgc agcgagcatc aggccaaact
      961 ggtcggcgcc gatcctgatc acgatttagc cgtcctccag attcaggccc ccgagagcct
     1021 gctggaacca ttagccatcg gcagctcaca cgatctccgc gtcggtcaaa aagtactggc
     1081 cattggcaat ccgttcggcc ttgatcatag cttgaccaca ggagtcgtca gcgcgctggg
     1141 tcgcacgatc aaatcgctgt cgaaccggac cattgaaggg gtagtgcaga ctgatgcagc
     1201 gatcaatcct ggcaactcag gcggaccgct ccttgatagc gcggggcgct tgatcggcgt
     1261 gaatacacaa atcatcagcc ccagcggagc ctacgcggga gtcgggttcg ccgtcccggt
     1321 cgataccgtc aatcgcatcg ttcccgagct gatcaagcat ggcaagctca ttcgtccggg
     1381 gctcggcatc tccctcgtgc cagatgccat tgtcaggcga tggggcatca aggggttggt
     1441 cattggcaag gtcactcagg gcggtgcagc ggatcgggcc ggactcagga gtgcccgaga
     1501 aacccccaca ggacgaatag aactcggcga catcgtggtc gccgtggccg gaaaaccggt
     1561 cacgaccatc gacgagctga tggcggtgtt ggaagatcac aaggtcggcg aacaagtgat
     1621 cgtggagatc ttgcgaggaa accgccgcga aaagatcacg gtgacgcttc aggccgtcaa
     1681 ttagcaggcg acaatctccg atctctctat ggtaggatct tggtctcatg tggaggacca
     1741 tccatgagtg atcgccgctc agccgactcg cctcaagagc cgaccagcag acgggaagat
     1801 ccgggcacga agaccggttc tgatcccctc gagctcattg accagtgtct cgcgcttttc
     1861 cccgagtcag atccccggca gaagctactg tataagctgc gtcatgtggt gatgctctcc
     1921 caagctgcca atcagcagcg ggaagccgaa ttcagaaaag tcagcgaagt ggttgccaag
     1981 ctcacggcac ccgccaaccg gatcggcatg ctgctggctt tgcccggcga agacctcgcc
     2041 cacatcctcg tggggggcgc cgaatattat gcgaatgtcg atccgcgagt cgccaccggc
     2101 gacctcaaaa tcgggacgca gattcttgtg aacgaagcct atgcggtgat caagacactc
     2161 ggctacgacc ggaacggccc gatcttgaaa ctgacggaag ccatggccga cggccggctg
     2221 cggtttgagc aggaaatggg acggcaatcg ctgatcttgc agcgatcgag cgacttggtc
     2281 ggtgtggagc tgaaggcggg cgacgaagtc cggatcgaca ccagtctacg cattgccatc
     2341 gagaaactgg cggaccgcaa ggccgccaaa catgtgctcg acgaagcgcc gaacgtcacg
     2401 tgggagcaaa tcggcggaca gcaggaagcc atcgcggcga ttcgaaaggc gatcgagtat
     2461 ccgttgctgc atgcctccac gttcgagcag ttcaagttct cccaacccaa aggatttctc
     2521 ctctacggcc cgcccgggtg cggcaagaca ctcatcggcc aggcggctgc tgcgagcctg
     2581 tccaagctgg tcagcgagtc tggtccggac gtgcacgggg ggcgcgacac gcgcgctccc
     2641 atcaccggtg gcgcgttcct ccatgtgaag ggcccagaga tcctcaacat gtggctaggc
     2701 gaatcggaac gcatggtgcg cgatctgttt gcgcaggcaa gagccaagcg ccacgagggg
     2761 gctctcccat tcatctttat cgacgaagcc gagtccatcc ttgggaccag gcgggccatg
     2821 cggtccttca acatctccaa taccttggtg ccgatgtttt gcagcgaaat ggacggcatc
     2881 gagtccctgc gcgatgtcgt cattattctg gcctcgaacc ggccggattt gattgacccc
     2941 gccgtccttc ggcctggccg tatcgatcgc aagatcaaag tcgcgcggcc caatcgcgaa
     3001 gcctcagctc agattttgaa ggtgtacctg cgccatgact tgccactcga tcctgcgttg
     3061 cttgcggagc gcgggggaga gcagggaaga gtcatggctt cgctcgccga cgacgtgctc
     3121 gaacaagtct tcaaaagaag tgatgagaat cgattgctgt ccgtccggct ccgaaacggc
     3181 cagcagaaag tgctctaccg aggggacctg gtcagcggcg ccatattggc gtcaatcgtt
     3241 cagcgggcca aggaaaaggc catcgaccgc acgattcaat ccgggcagcc agccgggctc
     3301 ctgtctcagg atctgtttga tgcagtcacg gaggaatttc gag
//
LOCUS       DASTQY010000141         3772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1521214, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000141 DASTQY010000000
VERSION     DASTQY010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521214"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VFR82_04545"
     CDS             complement(<1..807)
                     /locus_tag="VFR82_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500899.1"
                     /translation="MRRVGPPPTALCLFIAVSLVPAAWADSINEKIDRERKTLEQLKD
                     QIEEKRRQADEAGKKRESILQGIQTLDERHMRYRQSRQDISRKLRKKDQEIETINAQI
                     ARLREGVQVRQQAILARLRVQYMEGRFGHWKPLLASDSYGDFQRRLRYLSAVSERDYS
                     LIETFKSDMATMQEAERQREAARVGMLAYKRSTEKHLDEINAVKKEKKLYLTKLTQEK
                     ESYERALQELERSASRIDSLLRELEQRRRAAAAKPPSGSLQRGVKGGLPWP"
     gene            complement(804..1703)
                     /gene="ftsX"
                     /locus_tag="VFR82_04550"
     CDS             complement(804..1703)
                     /gene="ftsX"
                     /locus_tag="VFR82_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEU4500900.1"
                     /translation="MRTLSYLLREAWANIWTNRTTTVVAIFTTAFTLACVGVFLLLYV
                     NLRAAAGWLQDDIKIMVYLAEGIAGSTVSDIEQQLRADRAVGTLHFISKEQALGEFRA
                     QFPSDSHLLEGLGQNPLPASFVVTLAPAFRSPDAVRRWAERVAAVAGVAKVDYNQDWL
                     DALSTVIRSIEVVAIGIGLILSAAAVTIIANTIRLTLFARRDEIAILRLIGATKTFIR
                     IPYLLEGAVLGALGSTCSLLMLKALYELFRQQMRTTGRLRGLEQLTVFFPLSICLLLV
                     AVGIILGCAGSFLSLRRFDEAKV"
     gene            complement(1700..2368)
                     /gene="ftsE"
                     /locus_tag="VFR82_04555"
     CDS             complement(1700..2368)
                     /gene="ftsE"
                     /locus_tag="VFR82_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU4500901.1"
                     /translation="MIQLVHVTKQYDRRPALCDVTLEIPKGEFVLLMGPSGAGKSTLL
                     RMLIGAERPDVGQVFVNGKNVTTLKGVEIAYHRRKVGTVFQDFRLLEKKSVFDNVALP
                     LLVQGISRTDIKRKVTEALRAVGVEHKRDQLPMSLSTGEQQRVCIARAIVNGPVVLLA
                     DEPTGNLDPALTGEIIDLFKLINARGTTVVVATHDPSVMTQVNRRVVTLVQGSLMPEL
                     RVGV"
     gene            complement(2376..2768)
                     /locus_tag="VFR82_04560"
     CDS             complement(2376..2768)
                     /locus_tag="VFR82_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEU4500902.1"
                     /translation="MTILDPRRLFGQEGEETAERYLRQKGYRIVAKNLRTSLGELDLV
                     AEDGDVLVFVEVKARRTTDFGGAVHAVHRQKQTQLVRLAAQFLARHHWSNRPCRFDVV
                     LLQSLQTAGAQVEHIQNAFEVSGDDLRW"
     gene            complement(2770..3453)
                     /locus_tag="VFR82_04565"
     CDS             complement(2770..3453)
                     /locus_tag="VFR82_04565"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU4500903.1"
                     /translation="MTARILNFVGPTNEFELEARRCGYRRVAGLDEAGRGPLAGPVVA
                     SAVVLPSRCRLIGCDDSKLLSESEREQLYVVIRKRAVSIGIGCATEQEIDRLNILEAT
                     RLAMHRALAGLMPKPDCLLIDAISLPACPIPVRSIIKGDALCMSVAAASIVAKVTRDR
                     LMAEYHRVYPHYNFLSHKGYGTEEHLLQLATHGPCQIHRRTYAPVADVIARSRDVLPQ
                     HVQQELDGQ"
     gene            complement(3490..>3772)
                     /locus_tag="VFR82_04570"
     CDS             complement(3490..>3772)
                     /locus_tag="VFR82_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU4500904.1"
                     /translation="KGSLNTEMFTVRKVSYGVGVERIFPVHSPIVTKIDVVRQGKVRR
                     AKLYYLRGKKGKFSRVEDREFVGESKASAGSPRVTEPAEEPVEAAAAKS"
BASE COUNT          772 a         1157 c         1048 g          795 t
ORIGIN      
        1 aggccacggc aagcctcctt tcacgccgcg ctgcaggctt cctgagggag gcttggcggc
       61 cgcagctcgg cggcgttgtt ccagctcgcg tagaaggctg tcgatccgtg aggcggatcg
      121 ctcgagctcc tgcaaggccc gctcgtagga ttccttttcc tgcgtcagtt tggtcaggta
      181 cagctttttt tctttcttga cggcgttgat ctcatccaga tgcttttctg tgctgcgctt
      241 ataggccagc atgccgacgc gcgccgcttc ccgctgtcgt tcggcttcct gcatggtggc
      301 catgtcgctc ttaaaggtct cgatcagact gtagtcccgc tcggaaaccg ccgacagata
      361 gcgcaggcgt cgttggaaat ccccgtacga atcggacgca agcaacggct tccaatgtcc
      421 gaaccgtccc tccatatatt gcacacggag cctggccaag atcgcctgtt gcctgacctg
      481 cacgccctcg cgcaagcgcg caatctgggc gttgatcgtc tcgatttctt gatctttttt
      541 tcgcagcttt cggctgatgt cctgacggga ctgccgatac cgcatgtgtc gctcgtccag
      601 cgtctgaatt ccctggagaa tggactcccg cttcttgccc gcttcgtcgg cctggcgtct
      661 tttttcttcg atctggtctt tcagctgttc caaagtcttt cgctccctgt cgattttctc
      721 attaatcgaa tcggcccatg ccgccggcac caggcttacg gcgatgaaca ggcaaagggc
      781 ggttggagga ggccctaccc gcctcatact ttggcctcgt cgaaccgccg aagggacaag
      841 aagcttccgg cgcagcccag gatgatcccg acggcgacaa gcaaaagaca gatcgagaga
      901 ggaaagaaca cggtgagttg ttccagccct cgcaaacgcc cggtcgttcg catctgttgc
      961 ctgaacagct catacaaggc cttcagcatg agcagtgagc aggtgctgcc caacgcgccc
     1021 aggactgcac cctccagcaa ataggggatg cgaatgaagg tcttcgtcgc gccgatgagc
     1081 cgtaggatgg caatttcatc ccgtcgtgcg aacaaagtaa gtctgatggt attggcgatg
     1141 atcgtcacgg ccgctgccga gaggatgagg ccaatcccaa tggcgactac ctcgatcgac
     1201 cgaatgaccg tggagagggc atcaagccag tcctgattgt aatccacttt cgcgacgccg
     1261 gcgacagcgg cgacgcgttc cgcccaccgt ctgaccgcgt ccggcgagcg gaaagccggt
     1321 gccagcgtca cgacgaagga ggccggcaaa ggattttggc ccaacccttc caggagatga
     1381 gaatccgacg ggaactgagc tctgaattct cccagtgctt gctccttgga aatgaaatgc
     1441 aatgttccca ctgcccgatc ggcgcgcaat tgctgttcga tgtccgacac cgttgagcca
     1501 gcaattccct ccgccaagta caccatgatc ttgatatcgt cctgcagcca gccggcggcc
     1561 gcgcgcagat tgacgtacag cagcaggaag acgccgacac aggcaagcgt gaatgccgtg
     1621 gtgaaaatcg ccacgacggt cgtcgtgcga ttggtccaaa tgttggccca ggcctctcgc
     1681 aacagataag acagcgtcct catacaccga cccgcagctc cggcatgagg gaaccttgca
     1741 cgagcgtgac aactcggcga ttgacttggg tcatcacgga cggatcatgc gtggcgacta
     1801 ccaccgtggt tccccgtgca ttgatgagtt tgaacaagtc gatgatttcg cctgtcagtg
     1861 ccggatcgag gttgcccgtc ggttcgtccg ccaacagcac cactggtcca ttgacgatcg
     1921 cccgcgcaat gcaaacacgt tgctgctccc ccgtcgaaag actcatgggc agttggtcgc
     1981 gcttgtgttc cacgccgact gcccgcaacg cttccgtcac cttacgcttg atatcggtgc
     2041 gggagatccc ttggacaagc aatggcagag ccacgttgtc gaatactgat tttttctcca
     2101 acaatcgaaa atcctggaag accgttccga ccttgcgtcg atggtaggca atctcgacgc
     2161 ccttgagcgt cgtgacgttc tttccgttca cgaacacttg tccgacgtcc ggccgctcag
     2221 cgccgatcag catacgcagc agtgtagact tcccggctcc gctcggaccc attaatagga
     2281 cgaattcgcc cttcgggatt tcaagcgtga catcgcatag ggcgggccgc cgatcatact
     2341 gcttcgtgac atggacgagt tggatcatcg cattttcacc agcgtaaatc gtcccctgag
     2401 acctcaaagg cattctgaat atgctcgacc tgcgcgcccg ctgtctgcaa gctctgaagc
     2461 agcacgacgt cgaaccgaca aggacggttg gaccaatgat gccgagcgag gaactgcgcc
     2521 gccaagcgca ccaattgagt ttgcttctgc cggtgaacgg catgcaccgc cccgccaaaa
     2581 tccgtcgttc ggcgtgcctt gacttccacg aacacgagca cgtcgccatc ctccgccacc
     2641 agatccaatt cgccaaggga cgtgcgcaga ttcttggcga caatccggta gcccttctgg
     2701 cgcaaatagc gctcggccgt ctcctctcct tcttgcccaa agagtcgtct agggtcaagg
     2761 attgtcacgc tattgtccgt cgagctcctg ctggacgtgc tgaggcagaa catcacggga
     2821 ccgggcaatc acgtcagcca cgggggcata ggtccggcga tggatctggc acggcccatg
     2881 tgtcgcaagc tgcagcaaat gctcttccgt tccgtagccc ttgtgagaga gaaaattgta
     2941 atgcggataa actcgatgat attcggccat gagacgatct cgggtgacct tcgctacaat
     3001 cgacgccgcg gccaccgaca tacatagcgc gtcacctttg atgatcgaac gaacgggtat
     3061 gggacaggct ggaagcgaaa tcgcatcaat cagcaggcaa tcaggcttcg gcatgagccc
     3121 tgcgagggca cgatgcatgg ccagtcgcgt agcttccaga atattcaacc ggtcgatctc
     3181 ttgctcggtc gcacaaccaa tgccgatgct gaccgcccgc ttcctaatca ccacgtacag
     3241 ttgttcgcgt tcggattcgg aaagcagctt ggaatcatcg cagccgatca gacggcaccg
     3301 gctgggcagg acgacggcgg atgctacaac aggaccggcc aatggacccc ggccggcttc
     3361 atccaggccg gcgacacggc gatagccgca ccgccgcgct tccaattcga attcatttgt
     3421 gggacccacg aagttgagta tgcgtgcggt cacccgcgac cctcgtggcc ggagaatgga
     3481 tgatgtcggt caggatttcg cagcggctgc ttcaaccggt tcttccgctg gctcagttac
     3541 gcgcggagag ccggcgctcg ccttgctctc tcccacgaat tcgcgatcct caacccggga
     3601 gaacttgccc ttcttgccgc gtagataata cagcttggcg cgccggactt ttccctgacg
     3661 gacgacatcg atcttcgtga cgattggtga atggacgggg aaaatacgct cgacgccgac
     3721 accgtaggaa accttgcgca ccgtgaacat ttccgtattg aggctcccct ta
//
LOCUS       DASTQY010000142         3006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1277382, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000142 DASTQY010000000
VERSION     DASTQY010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277382"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(319..714)
                     /locus_tag="VFR82_04575"
     CDS             complement(319..714)
                     /locus_tag="VFR82_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4168 domain-containing protein"
                     /protein_id="HEU4500905.1"
                     /translation="MKLPPVITGVFLGAILCTGVGLAAEPAEVEKYVKARIEIGEMMT
                     NYFKGGEGAPPTQEQRREMGKDINAKLSTVLSKYGLTVEEYRAHSREVFADQAAVTGF
                     LNQHPDLKQRYEALPLDRMGRGGSTGRGY"
     gene            complement(977..2209)
                     /locus_tag="VFR82_04580"
     CDS             complement(977..2209)
                     /locus_tag="VFR82_04580"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEU4500906.1"
                     /translation="MSGEPIEIRVSQPTAGSDEPTFPPYNGPAFFSYGFRPFFLGAAV
                     FAGVAVPVWILILSGAIGSSFLYAPREWHVHEMLFGFLPAVMTGFLLTAIPNWTGRTP
                     LRGMPLASLWMLWLAGRLVVAVSTPAPVLAAVIDASFLVMLAALVWREIAAAGMWNRS
                     PIGALITLYAAANMLFHTLALHAVPTDVPERLALSILFMLLTVIGGRVTPAFTGEYLK
                     EQRITSSPAPFSLFDKLSMLLVLIAVLAWNVQPAGDAAGVLFIAAGVLTLVRLSRWRG
                     WMAWREPLVFILTVGYGWVGLSLLALGGASLGMLPAANAVHVLTTGAVGTMTLAILTR
                     ASLGHTGRTRHAGLMTVVIYVLVNVGAILRIVSPASDAPTALTHLVLGLSALAWSGAY
                     LLFALVYGSILVRPSLHE"
     gene            complement(2354..>3006)
                     /locus_tag="VFR82_04585"
     CDS             complement(2354..>3006)
                     /locus_tag="VFR82_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEU4500907.1"
                     /translation="PVRMLLVKTVQVVILLFAVMAALGQLGVQIAPLLAGVGVAGLGI
                     GLALQGVFSNVVAGLSIIFTKPYRVGEHISLLGVEGDVLAINLFSTILKHPDRSLVVI
                     PNRKIVGEILHNFSTIRQLHLKVGVAYSTNLTEALEVIRQILAQNAGVLTDPPPAIGI
                     SELGDSAIKLSIDPWVAVVNFVKVEGELNQAIIERFRSARIEIPFPQRGVRLLSTP"
BASE COUNT          711 a          884 c          886 g          525 t
ORIGIN      
        1 ggttatccga aagcgggtga acacgtagag gactcaggcc acggggaaac cgccggtcaa
       61 aggcgaagct cggagcacac caagaatgcc gccaggccaa tcagtacaca aacactgagc
      121 agaacattct tcgtggacca ccatccctaa cacgatgagt tggagaactg cattgcaaag
      181 accaggagcc agacggttgc cgtgatggcc cgcaaaaacg aggaggacaa aggtattcgc
      241 ggcaacgtcg gggggctgag cgagaaccgt caccccggtc gaaagaatgt agatctgtag
      301 cagagaggag tcgtggtctt agtatccgcg tccggtgctg ccgcccctgc ccatacgatc
      361 caacggaagg gcttcgtagc gctgcttcag atcgggatgt tggttcagga agcctgtcac
      421 cgccgcttgg tcggcaaaga cttctctgct gtgggcacga tactcctcca ccgtcagccc
      481 gtacttggaa aggacggtgc tcagtttcgc gttgatgtcc ttgcccatct cgcgcctctg
      541 ctcttgagta ggaggggctc cctctccgcc tttgaaatag ttcgtcatca tctcgccgat
      601 ctcgatacgg gccttcacat acttctcaac ctcagccggc tcggcggcaa gaccgactcc
      661 ggtacagagg atggcgccca ggaacactcc ggtaataaca gggggcaatt tcatcgtgga
      721 atctcctatt tgctccattc tcagtgccgc taaatggctt gtcaatgagc gcctgtggct
      781 gattgtcgta acttcgatgg gtgcggggct caatatgcgc cttcttcccg gtgcactgcc
      841 tatacatggt ccttccaagc tcgcttgctt tctcttcaag gcggggctgg tttgatctcc
      901 actgcgcgct tggacgaggc ccttctcagg gcgcgcgttc ggcgagcaca ggagatcaac
      961 cagccctcac gcccctttac tcatggagac tgggacgaac gagaatcgat ccatagacca
     1021 gggcgaagag gaggtaggcg ccgctccaag cgagtgccga taggccgagc accagatgtg
     1081 tcagagccgt cggagcatcg gaagccggcg aaacaattcg caggatcgca ccaacgttga
     1141 ccagcacata gatgacgacg gtcatgagac cggcgtggcg cgtgcgaccc gtatgaccca
     1201 agctcgctcg tgtcaagatc gccagcgtca tcgtgccgac ggcgccggtc gtgaggacat
     1261 gcacggcgtt cgcggcgggc agcatgccaa ggcttgcacc tccgagcgcc agcagcgaca
     1321 gccccaccca cccatagcca accgtgagaa tgaacaccaa tggctctcgc cacgccatcc
     1381 accctcgcca acgggaaagg cggacaaggg tcagcacgcc cgccgcgata aacaagactc
     1441 ctgctgcgtc acctgccggc tgaacgttcc acgctagaac ggcaatcagg acgagcagca
     1501 tcgacagttt atcgaaaagg gaaaatggag cgggcgacga agtgatgcgt tgctctttca
     1561 ggtattcgcc agtgaaggcc ggtgtcacgc gcccaccgat gacggtcaac aacatgaaga
     1621 ggatcgaaag cgccaggcgc tcgggcacat cggtcggcac ggcatgcagc gcgagtgtgt
     1681 gaaacagcat atttgcggca gcatagagtg tgatcaacgc tccgatcggc gaccggttcc
     1741 acattccagc tgccgcaatc tcgcgccaca cgagcgcagc caacatgacc agaaagctcg
     1801 catcgatgac cgcggccaga accggagcgg gcgtcgagac tgcgacgacc agccgaccgg
     1861 ccagccagag catccacaag gacgccaacg gcatgcctct cagcggagtg cgccctgtcc
     1921 agtttggaat cgcggtcagg agaaaaccgg tcatcacggc gggcaggaaa ccgaatagca
     1981 tctcgtggac gtgccactcg cggggagcat agaggaagct ggagccaatt gcgccggaaa
     2041 ggatcaatat ccagacaggc acggcgacgc ccgcgaacac cgcggccccg agaaagaatg
     2101 gccggaagcc atatgagaaa aacgcggggc cattgtaagg agggaaggtc ggttcatccg
     2161 acccggctgt cggctggcta accctgatct cgatcggttc gcctgacacg ggaggcattg
     2221 tcgagaacag gccgaagcat tgtcaacttc ttgtcttcat agcccgattc ctcacatgtg
     2281 ttcgcacttg caactccatg agtaaggcaa tcactccatc ccttttcatg gacggagggc
     2341 gcggcaatga ggatcagggc gtcgaaagca gacggacccc gcgctgcggg aaaggaatct
     2401 caatgcgtgc cgagcgaaat cgttcgatga tggcctggtt cagctcccct tccaccttca
     2461 cgaaattgac gactgctacc cacggatcga tggaaagctt aatagcagag tcgcccagtt
     2521 cggagatgcc gattgcaggc ggaggatcag tcaggacgcc ggcattctga gcgagaatct
     2581 gccggatcac ctcgagcgcc tcagtgagat tagtggaata ggccacgccg actttaagat
     2641 ggagttgtcg gatggtgctg aagttatgga ggatttcacc cacaattttg cggttcggaa
     2701 taacaacgag cgagcgatcg ggatgcttaa gaatggtcga gaagagattg atggcgagca
     2761 catcgccttc aacaccaagc agcgagatat gctccccgac ccggtacggt ttggtaaaaa
     2821 tgatagagag cccggccacg acattgctga agacgccttg caacgccaag ccgatgccga
     2881 gtcctgcaac accgacgccc gcgagcagcg gcgcgatctg cacgcccaac tggcccagcg
     2941 cggccatgac ggcgaacagc aaaatcacca cctgcacggt tttgaccagc agcatccgaa
     3001 ccggcg
//
LOCUS       DASTQY010000143         5579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_731455, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000143 DASTQY010000000
VERSION     DASTQY010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731455"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VFR82_04590"
     CDS             complement(<1..1295)
                     /locus_tag="VFR82_04590"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4500908.1"
                     /translation="MKHDHTNPPEPTAVHGFSPGFAVLGLEARLLATLEALGYEEPTP
                     IQREAIPPLLAGQDVLGQAATGTGKTAAFTLPLLQRIVHGPKTKPSAIILVPTRELAI
                     QVGEAVGRYGKELRTSVLPLYGGQAIGPQMHALKRGVDVIVATPGRALDHIRRHTLKL
                     ESVQIVVLDEADEMLDMGFAEDLDAILEHTPTTKQTALFSATMPPRIQSIAHRHLQRP
                     VEITIAKEPVKAGSAPRVQQAAYIVTRPYRGATLARIIETAAPKSALVFCRTRVEVDE
                     VTLMLSARGHRVEAIHGGMSQVQRDRVMQAFRTGKTELLVATDVAARGLDIPQVSHVI
                     NYDLPSSAEVYVHRIGRTGRAGREGTAITILDPREQRLLRSIEQHTKGKVVVTPVPSV
                     ADVVAKRLERTRGAVQEAILAGDLDGFRGVVKAMAEATDL"
     gene            complement(1500..2180)
                     /locus_tag="VFR82_04595"
     CDS             complement(1500..2180)
                     /locus_tag="VFR82_04595"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HEU4500909.1"
                     /translation="MARSLGDPIQRLMGLMFKAHPWHGVSIGDKAPDVVTAYIEIVPT
                     DTVKYEVDKDSGFLKVDRPHRFSNYCPVYYGLVPQTFCGDKVAAIFSKRAKRKGMVGD
                     GDPLDICVLTEKTIPHSDILLTAIPIGGFSLADGGEADDKIIAVMKEDAAYGSFTDIR
                     DCPTTLIERLQHYFLTYKQAPGSLQHKVEITGIYGRDEALKVLRASHADYREKFPELE
                     SLWPKQMG"
     gene            complement(2212..2628)
                     /locus_tag="VFR82_04600"
     CDS             complement(2212..2628)
                     /locus_tag="VFR82_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500910.1"
                     /translation="MKPVIQLERTGCGIASVAALAGVSYQAARQAAGRLGICAEDHRL
                     WSETTHVRRLLKRYRLYASSGERPFRSWPALPRRSLLAIKWRRIRGRACWHWVVCVKD
                     GATYVLDSSPSLKRHVRTDFRRMKPKWFIAVTRLPS"
     gene            complement(2628..3098)
                     /locus_tag="VFR82_04605"
     CDS             complement(2628..3098)
                     /locus_tag="VFR82_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4500911.1"
                     /translation="MTTDIDLAGPEDAEVIAAMVGELLQEIMSAVSEKAFNFHHHDTE
                     ARARAWLIDGSYLVLLARHDEAAVGFLALYQSYALYTEGAYGTIPEFYVRPSHRSRGI
                     GTALIAESKKVGQARGWRRLEVTTPPLPQFDRTLAFYQRHGFAISGGRKLKVLL"
     gene            complement(3156..3353)
                     /locus_tag="VFR82_04610"
     CDS             complement(3156..3353)
                     /locus_tag="VFR82_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500912.1"
                     /translation="MAVYQWTKTKPLGAGWYWFRGEAHEADPFIVQVDGAGQFQWPDG
                     GYQEVRLAKGEWAGPIEEPHG"
     gene            3467..>5579
                     /gene="hrpB"
                     /locus_tag="VFR82_04615"
     CDS             3467..>5579
                     /gene="hrpB"
                     /locus_tag="VFR82_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HEU4500913.1"
                     /translation="MNRLPIEDVLPALSDTLVQGSNAVLSAPPGAGKTTRVPLALLDA
                     PWLTGKTILMLEPRRLAARAAAHRMASLLGESVGATVGYRMRLDSKIGPVTRIEVVTE
                     GILTRLLLEHPELERYAAILFDEFHERSLQADVGLALCLETQRLVRPDLRLLVMSATL
                     DCGPVSELLGDAPIITSEGRMFAVETRYLTHPLTGPLDAAVVQTIRRSLTQDQGSVLV
                     FLPGMAEIRRVERHLLDANLGPGVVVAPLHGDLPQPAQDKAIVPPESGTRKVVLATSI
                     AETSLTIDGVRVVIDAGQLRVPRFDPRSGLTRLETIRVTQDSADQRRGRAGRLESGIC
                     YRLWTEREHLSLAPRRTPDILEADLAPMVLDLAQWGEIDPSRLCWLNPPPSGAVAQAK
                     DLLIRLGALDASGRLTPHGRQMADLPLHPRLSHMLLQSLTLGCSDLACELAALLSERD
                     FVRGPSANRQTDVRLRIDVLQDIKKHVASGTIDRGTIERITRTARQWRTQLARFHRLP
                     VGGSSGTDVVGLLLALAYPDRIAQRQPGNEARYLLANGRGAIFAYPDPLASEPFLVIA
                     ALDAGTHWARIDLAAPLSPKEIEHLYADQIDEAEAVTWDDRTRSVRASRQRRFGALIL
                     SDEALSKTDPSLITKALLDGIRRAGLATLAWTPELLEWRRRIQFLRRLGDRGVSTSRG
                     REGEMGKRDVSASPGREGEMGE"
BASE COUNT         1081 a         1781 c         1571 g         1146 t
ORIGIN      
        1 agatcagtcg cctcggccat ggccttgacg actccgcgaa agccgtcgag atcccctgcc
       61 aggatcgcct cctgaacggc gcctcgcgtc cgctccaatc gcttcgccac gacatcggcc
      121 accgacggca ccggtgtcac gaccactttt cccttcgtat gttgctcgat gcttcgcaac
      181 agccgctgtt cccgcggatc gagaatcgta atcgcggtcc cttcgcgtcc cgcccgtccc
      241 gtccgtccga tgcggtgcac atacacttcg gctgatgagg gcagatcata gttgatcaca
      301 tgtgagacct gcggaatgtc gagtcccctg gcggcaacat ccgtcgccac aagcagctcg
      361 gtcttcccgg tccggaaggc ctgcatcacc cgatcccgct ggacctggct catcccccca
      421 tggatggctt ccacccggtg tccccgggcg cttagcatga gcgtgacttc gtcgacttct
      481 acacgcgtgc ggcaaaacac cagcgccgac ttgggcgctg ccgtctcgat gatccgtgcg
      541 agcgtcgccc cccgatacgg tcgtgtgacg atgtaggcgg cctgctgcac ccgcggcgcc
      601 gaaccggcct tgaccggctc cttcgcgatc gtaatttcta ccggtcgctg caggtgccga
      661 tgcgcgatcg attgaattcg cggtggcatc gtggctgaaa acaacgcggt ctgtttggtc
      721 gtaggcgtgt gctcgaggat cgcgtcgaga tcctccgcaa atcccatgtc gagcatctcg
      781 tccgcttcat ccaggacgac aatctggaca ctttccagct ttaacgtgtg gcgtcggata
      841 tgatcgagcg cacggccggg tgtggcaacg atcacatcga cgcctcgttt tagtgcatgc
      901 atttggggac cgatcgcctg ccctccgtag agagggagaa cgctggtccg tagttctttg
      961 ccatatcgcc cgacggcctc tccgacctga atcgccagtt ctcgggtggg caccagaatg
     1021 atagccgaag gctttgtttt tggaccgtga acaatgcgct gcagcaaagg cagtgtaaag
     1081 gcggctgtct tacccgttcc cgtggctgcc tgtcccaaca cgtcttggcc tgcaagtaaa
     1141 ggcggaatgg cttcccgttg aatcggcgtc ggctcctcat agcccaacgc ctccagcgtc
     1201 gcgagcaacc tcgcttcgag tccaagcacc gcaaacccgg gagaaaagcc gtgaacggca
     1261 gttggctccg gcggattggt atggtcatgc ttcatcaaaa tctcttttca cgacaagagg
     1321 ggcctgagga aaacgggcct atatatgatc cttcgaaatt tttcacgctg ctcatgaggg
     1381 ttgcccgatc ggtctcaaac gtgcgcgcgt cgacgagcac ctcccgcagt ctaatgttag
     1441 atcattctgg tgtgcgcgtt gcgcgagcac aagagaccga cgggcaaccc gtactccctt
     1501 caccccattt gcttgggcca gagcgactcc agttctggga acttttcccg gtagtcggca
     1561 tggctggctc gcaggacttt gagcgcctcg tcacgtccat agatgccggt gatctccact
     1621 ttatgttgca gggacccggg cgcctgcttg taggtaagga aataatgttg gagccgctcg
     1681 atcaatgtcg taggacaatc acggatatcc gtaaaacttc cgtacgccgc atcttccttc
     1741 atgacggcga taatcttatc gtccgcttcg ccgccatctg ccaagctgaa tccgccgatg
     1801 ggaatcgcgg tcagcaaaat gtcgctgtga ggaatggtct tttccgtcag cacgcagata
     1861 tccagcggat caccgtcgcc gaccattcct tttcgcttgg ctcgtttgga aaagattgcg
     1921 gccaccttgt ccccgcagaa ggtttgggga acgagaccat aatagacggg acaatagttg
     1981 gaaaatcggt gcgggcgatc gacttttaag aatccgctgt ctttatcgac ttcatacttc
     2041 accgtatccg tcggcacgat ctcgatgtac gccgtcacga cgtccggcgc cttatccccg
     2101 atcgacactc catgccaggg gtgcgccttg aacatcagac ccatcaaccg ttgaatcgga
     2161 tcccccagag aacgcgccat caattcctcc cccccaatgt cagagaacgt ttcacgaagg
     2221 caatctcgtg acggcgataa accattttgg cttcatgcgt cggaagtccg tcctcacatg
     2281 acgcttgagc gacgggctcg agtcgagaac atacgtcgcg ccatccttta cacacaccac
     2341 ccaatgccaa cacgctcttc ccctgatgcg acgccacttg atcgccagga gagaccgcct
     2401 aggcaatgcc ggccaggacc ggaacggccg ctctccggaa gaggcgtaga gacgataccg
     2461 cttcagcaac cgccgcacgt gagtggtctc ggaccagagg cggtgatcct cggcgcaaat
     2521 tcccaaacga cccgcagctt gccgcgccgc ctgataactc acaccggcca gtgcagcaac
     2581 gctggcgatc ccacagcccg ttcgctccaa ctggatgacc ggcttcatca caacaatacc
     2641 ttcagtttgc gtccgcccga gatcgcgaat ccatggcgct gataaaatgc cagcgtccga
     2701 tcgaactgcg gcaagggagg cgtggtgact tcgaggcgcc gccatcctct ggcttgtccg
     2761 actttttttg attcggcaat taatgccgtc cctattcccc gcgaacgatg cgacggacgg
     2821 acatagaatt ccggaatcgt gccgtacgct ccctccgtat acaacgcata actttgatac
     2881 agggccagga atcccacagc agcttcatcg tgtcgcgcca gcaagacgag ataggagccg
     2941 tcaatgagcc aggcacgagc gcgagcttcc gtgtcgtggt ggtgaaagtt gaaggccttc
     3001 tcgctcacgg ccgacatgat ttcctggaga agctctccaa ccatggcagc gatgacctcc
     3061 gcgtcttccg gcccagcgag atcaatgtca gtggtcacgg gagatgtgtc tcggtgtgct
     3121 gtctgcatgt cacacaatgg tccgcccatg caggttcagc catgaggctc ctcaatcggc
     3181 cccgcccatt cgcccttggc caatcgtacc tcttgatacc ctccgtcagg ccactggaat
     3241 tgtcccgccc catcgacctg cacgatgaag ggatcggcct catgggcctc accgcggaac
     3301 caataccatc ctgctcccag tggcttggtc ttcgtccatt gatacaccgc cattgatctc
     3361 tccatccttt gcgcgccgct tgtccacaga ctcgtcccgc gccgggacgg cacattcctt
     3421 ctcacagcag gcatctgtta cagtccgact cgtgacgaac cctagcatga accgtctgcc
     3481 aatagaagat gtccttccgg ccttgagcga taccttggtg cagggcagca atgccgtctt
     3541 gagcgcgcca ccgggtgccg gaaaaaccac ccgcgtgcct ttagccctgc tggatgcgcc
     3601 ttggctgacc ggaaaaacga tcctcatgct ggaaccgagg cggctcgcgg ccagagccgc
     3661 ggcccatcgc atggcctcct tattaggaga atcggttggc gcgactgtcg gttatcggat
     3721 gagattggac agcaaaattg gaccggtcac tcgtattgag gtagtcaccg agggcattct
     3781 cacccggctg ttgctcgaac atccggaact agaacggtac gccgcgattc tcttcgatga
     3841 gttccacgaa cggagcctgc aggccgatgt tggcttggcc ctctgtctcg aaacccagcg
     3901 gctcgttcgt cccgatctcc gtctgctggt gatgtccgcg accttggact gcgggcccgt
     3961 gagcgagctg cttggcgatg caccgatcat cacttctgaa ggccggatgt ttgccgtcga
     4021 aacgcgctat ctgacccatc cgctcaccgg tccgctcgac gcggcagttg ttcagaccat
     4081 ccgtcgatcc ctcactcagg accagggaag cgtcctggtc ttcctgccgg ggatggcgga
     4141 aattcgccgc gtcgaacgac acttgctcga tgcgaatctc ggaccaggcg tcgtggtcgc
     4201 tccgctccac ggcgatttgc cacaacctgc gcaggacaag gcgattgtgc cgccggagtc
     4261 cggcacgaga aaagtcgtat tggcgacatc catcgccgaa accagcttaa cgatcgacgg
     4321 cgtccgcgtc gtgatcgatg ccggtcaatt gcgagtcccc cggttcgacc cgcgctcggg
     4381 attgacgcgg ttggagacca tccgcgtgac gcaggattcg gccgaccagc gcagaggcag
     4441 ggcgggccgg ctggaatcgg gtatctgtta ccgcctgtgg accgaacgag agcacctgtc
     4501 actcgcacca cgaagaactc ccgacatctt agaggctgat ctcgctccaa tggtcctcga
     4561 cctcgctcaa tggggagaga tcgacccttc ccgcttatgc tggctcaacc cgcctccttc
     4621 cggagcggtc gcacaagcga aggacctttt gattcgtctg ggcgcgctgg atgccagtgg
     4681 acgactgacg cctcatggca ggcagatggc cgatctgccg ctgcatcccc ggctctcaca
     4741 catgctgctc cagtccctga cgctgggctg ttccgacctc gcgtgcgagc tggccgccct
     4801 cttgagcgaa cgggacttcg tacgaggacc gtcggccaac cggcagacag acgttcggct
     4861 ccggatcgat gtactgcaag acatcaagaa gcatgtcgcc agcggcacca tcgatcgagg
     4921 aacgatcgag cgaattacac gaaccgcccg ccaatggaga acgcagcttg ctcgatttca
     4981 ccggctaccg gtaggcggat cttcggggac ggacgtcgtc ggcctgctgc tggctctcgc
     5041 ctatcccgat cggattgcgc agcggcaacc gggcaatgaa gcccgatacc ttctggccaa
     5101 tggtcgtggc gccatttttg cgtatccgga cccgctcgcc tctgaaccgt ttctcgtcat
     5161 cgcagcattg gacgccggca cacattgggc gcgcatcgat ctcgctgcgc cgctgtcgcc
     5221 caaggagatc gaacacctct atgccgatca aatcgacgaa gccgaagcgg tcacgtggga
     5281 tgaccgaacc cggtcggtgc gcgcctcgcg acaacgccgt ttcggcgccc tcatcttgtc
     5341 tgatgaggcg ctctcgaaga cggacccttc cttgatcacc aaggccttgc tcgacggcat
     5401 tcgccgtgca ggcctggcca cgctcgcttg gacgccggaa ctcctagaat ggcgcaggcg
     5461 aattcagttc ttgcggcgcc tgggtgaccg cggcgtttcc acgtcgcgag ggagggaggg
     5521 tgaaatgggt aaacgcgacg tctcggcgtc gccagggcgg gagggtgaga tgggtgaac
//
LOCUS       DASTQY010000144         4489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1414917, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000144 DASTQY010000000
VERSION     DASTQY010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414917"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1807
                     /locus_tag="VFR82_04620"
     CDS             224..1807
                     /locus_tag="VFR82_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU4500914.1"
                     /translation="MDIHALQQIVHSTRDTRRKQTIAKFSPADRDQLIHKYHPDYRAS
                     AYRPIRFGPNKGDRTVVELATLLEGDSPIHDDQDLTPHYTTDVLVIGGGGAGCAAALH
                     AHGAGSQVMLATKLRLGDSNSVMAQGGMQISVAPEDSPVTHFIDTLKGGHMQNDHALL
                     KVMVEDGPAIAKWLIELGVLFDRQADGNLHVKKGGGSSKPRLLTCSDYTGLEIMRVLK
                     DEVINQKIQLLEFCAAVELLSDEDGRCSGAVFKDLDNQRNVIVAAKSVILATGGIGRL
                     HIQGFPTSNHYGATGDGLCLSYRIGAKLAHVDTFQYHPSGAVYPEQLIGALVTEGIRS
                     EGGHLVNAKGERFVNELDTRDVVSSSIIRECEEGRGIRTMSGRVGVWLDTPLLDAEHG
                     PGTVEKHFPAMMMQFERFGIDISKDPVLIYPTLHYQNGGVKIDTNSESDVKNLFVAGE
                     ASGGLHGRNRLMGNSLLDLMVFGKRSGLTAAARAASMKQEKLTLKHLQQFRAEAKKHG
                     NPSGVISPMILPAYTRKVG"
     gene            2031..3209
                     /gene="sucC"
                     /locus_tag="VFR82_04625"
     CDS             2031..3209
                     /gene="sucC"
                     /locus_tag="VFR82_04625"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEU4500915.1"
                     /translation="MNVHEFQAKSLFAQFGVPVPRGKEITTPEAAKAWADELNTPVFV
                     VKAQIHAGGRGKAGGVKITKDKAAVAGLAKELIGKTLVTHQTGPKGRKVHRLLMEEGA
                     NIAKELYLSILVDRDTGWPTFIASTEGGMEIEEVAEKTPDKIIKEAVDPAVGFQGHNG
                     RNVAFALGLQNIEPTVINPFVQMLGNLYRLFMDKHAALVEINPLIITKEKTLVALDGK
                     VSFDDNGLFKHEDVQKMRDLNEEEPLEIEATANNLNYVKLDGNIGCMVNGAGLAMATM
                     DVIKLAGSEPANFLDVGGGATKETVAAGFRILLKDPNVKGIFINIFGGIVRCERIAHG
                     VIEAAKEVKINVPLVVRLQGTNADEGRKLLAESGLKLEVANDLWEAAQKIVKLTGKAA
                     "
     gene            3315..4187
                     /gene="sucD"
                     /locus_tag="VFR82_04630"
     CDS             3315..4187
                     /gene="sucD"
                     /locus_tag="VFR82_04630"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEU4500916.1"
                     /translation="MSILVNKNTRVVVQGITGKEGSFHATQCKAYGTKVVAGVTPGKA
                     GQEVEGIPVFNTVADAVKKTESDTSLIFVPPPFCADAILEAADAGIKLIICITEGIPV
                     NEMVKVKRALRGRDVRLIGPNCPGVITVDEAKIGIMPGFIHKKGIVGVVSRSGTLTYE
                     AVHQLSTLGLGETTCVGIGGDPVNGTGFVDVLPLFEKDPETQAIVMIGEIGGDAEEKA
                     AEYIKKHVKKPVVSFIAGITAPPGRRMGHAGAIISGGKGTANEKMKALEAAGVRVIKN
                     PAEIGTTVKKALGR"
BASE COUNT         1042 a         1185 c         1364 g          898 t
ORIGIN      
        1 gctcatttca ccgaaaagcc ggtgggcttg gacgcccgga tcaaggaaat cacagacggt
       61 cggtttaatg acgagtggaa taagattctc aagatgaacg accagcaact tgccgagcac
      121 tgtgccacgg tgaagtgaca agcactcggc caacgttacc gtcctatgcg cgacgcgccc
      181 ggtgtgttca tgacgaccgc tgactattga cggtcttgaa gagatggata tccacgcact
      241 tcaacagatt gttcacagca cgagggatac ccgtcggaaa cagaccattg caaaattttc
      301 tcccgccgac cgcgatcagc tgattcacaa atatcatccc gactatcgag ccagcgccta
      361 ccggccgatt cgattcggtc cgaacaaagg ggaccggacg gtggtcgagt tggcgacgtt
      421 gcttgagggt gacagcccaa tccacgacga ccaggacctg actccgcact atacgaccga
      481 tgtcctcgtg atcggcggcg gcggagccgg atgtgccgcg gcgttgcatg cgcacggagc
      541 cggatcgcag gtcatgctgg ccacgaagct tcggctcggt gattccaaca gtgtcatggc
      601 gcagggcgga atgcaaatct ccgtggcgcc tgaagattct cccgtcaccc acttcatcga
      661 tacgcttaag ggcgggcata tgcagaacga ccatgccctc ctgaaggtga tggtcgaaga
      721 cgggccggcc attgccaagt ggttgatcga actgggcgtg ctgttcgatc gtcaggccga
      781 cgggaatctc catgtgaaga agggtggcgg cagctcaaag ccacgactgt tgacctgctc
      841 ggactatacg ggcctcgaga tcatgcgcgt cctcaaggac gaggtgatca accaaaagat
      901 tcagttgctc gaattctgcg ccgcggttga gctgctcagc gacgaagacg gccgttgttc
      961 aggcgcggtg ttcaaggatc tcgacaacca gcggaatgtg atcgtggccg cgaagtccgt
     1021 gatcctggcc accggtggca tcggccggct gcacattcaa ggcttcccga ccagcaacca
     1081 ttatggtgcc actggagacg gactgtgcct ctcctaccgc atcggtgcga agttggcgca
     1141 tgtcgacacg ttccaatatc acccgtccgg cgccgtctat ccggaacaat tgatcggcgc
     1201 attggtgacg gagggcatac gctctgaggg cggtcatttg gtgaatgcga agggcgagcg
     1261 gtttgtcaac gaactcgaca cacgcgacgt cgtttcctcc tcgatcattc gagaatgcga
     1321 ggaaggcaga ggtattagaa ccatgtcggg gcgagtcggc gtgtggttgg ataccccttt
     1381 gctggatgct gaacatgggc cggggacggt cgagaagcat tttcccgcca tgatgatgca
     1441 gttcgaacga tttgggatcg acatcagcaa ggatccggtg ttgatctatc cgacattgca
     1501 ttaccagaat ggcggtgtga agattgacac caattcggaa agcgacgtga agaatctgtt
     1561 cgtcgccggt gaagcctccg gcggcctcca cggccgcaac cggttaatgg gaaattcact
     1621 gctcgatctc atggtgttcg gcaagcggtc cggtctcacg gcggctgcac gagccgcgtc
     1681 catgaagcag gagaagctta cgctcaaaca cctgcagcag ttccgtgcag aagccaagaa
     1741 gcacggaaat cccagtggcg tcatttctcc gatgatcctg ccggcgtaca cgagaaaagt
     1801 ggggtagact acaaccgcaa cattgcttgt ccccgcgcca atgcggcgtc acggaaatgg
     1861 gttccggcct atcgccaaaa tcaattggag ggtctccaag tcgaacgagt gctgtttggc
     1921 ggtggtcacg acgggtaaag agcgcaacgt ctcgcgccgg gctggcagat gagaacagtc
     1981 gcgcaggcag aaacgtaaca ccgtccagtc ttccatgaat gaggtagaag atgaatgtgc
     2041 atgagtttca ggcgaagtca ttgtttgccc agttcggtgt ccccgttccc cgcggaaagg
     2101 aaattactac gccggaagcg gcaaaggcct gggccgatga attgaatacg cctgtgttcg
     2161 tcgtgaaagc gcagattcat gccggcggga gaggaaaggc cggtggcgtg aagatcacca
     2221 aggacaaggc ggctgtggcc ggcctcgcca aggaactcat cggcaagacc ctggtgacgc
     2281 atcagaccgg accgaaaggc cggaaagtcc atcgcctgct gatggaggaa ggtgcgaaca
     2341 tcgccaaaga gctgtatcta tccattctcg tcgatcgtga cacgggatgg ccgaccttta
     2401 tcgccagtac ggaaggcggg atggaaatcg aagaagtcgc ggagaagacg ccggacaaga
     2461 tcatcaagga agccgtcgat cctgccgtgg gctttcaggg gcacaacggc cgcaacgtgg
     2521 cctttgcact cggtttgcag aacatcgagc cgacggtcat taacccgttc gttcagatgc
     2581 tcggcaatct ctatcgactt ttcatggaca aacacgcggc gttggttgag atcaatcctc
     2641 ttatcatcac gaaagaaaag acgctggtgg ccctcgatgg gaaagtctcg ttcgacgaca
     2701 acggcctttt caagcacgaa gacgtgcaaa aaatgcgtga tctgaacgaa gaagagccgt
     2761 tagaaatcga agccacggcg aacaatctca actatgtgaa gttggacggc aacatcggct
     2821 gcatggtgaa cggcgcaggc ctggctatgg ccacgatgga cgtcatcaag cttgccggca
     2881 gcgagccggc caacttttta gacgtcggcg gcggcgcgac caaagagacg gtggcggccg
     2941 ggttccgcat tttactcaag gacccgaacg tcaaaggcat tttcatcaac atctttggcg
     3001 gcatcgttcg atgtgagcgc atcgctcacg gcgtcattga ggcggccaag gaagtgaaaa
     3061 tcaacgtgcc gttagtggta cggctgcagg gcacgaatgc tgacgagggg cggaagttgc
     3121 tggcggaatc cgggttgaaa ctggaggtgg ccaacgattt gtgggaagcg gcgcaaaaaa
     3181 tagtgaaatt gacggggaag gcggcgtgag cggtctggcc gtccctacgc tgctccaacg
     3241 ctacggctcg acattcctga catacaagtg aactgaatgg cctgtctgtt taggcaatac
     3301 aaaaggaagc tctcatgagc attttggtca ataagaatac acgggtggtg gtgcaaggca
     3361 ttactggcaa ggaagggtcg tttcatgcga ctcaatgcaa ggcgtatggg acgaaagtcg
     3421 ttgctggagt cacccctggg aaggccggac aggaggtgga aggcatcccc gtgttcaaca
     3481 cggtagctga tgcggtaaag aagacggaaa gcgacacctc gttaatcttc gtcccgccgc
     3541 cgttttgtgc cgacgcgatt ctggaagctg ctgatgccgg aatcaagctg atcatttgta
     3601 tcactgaagg catcccggtc aacgagatgg ttaaagtcaa gcgtgccttg cgaggtcggg
     3661 acgttcgatt gatcggtccg aattgtccgg gcgtcatcac cgtcgatgaa gcgaagatcg
     3721 gcattatgcc gggattcatc cataagaagg gcatcgtcgg cgtcgtctca cgcagtggca
     3781 ccctcaccta tgaggcggta catcaactgt cgaccctggg tttgggggaa accacttgtg
     3841 tggggatcgg cggcgatccc gtgaatggga ccggctttgt ggatgtgttg ccgctgtttg
     3901 agaaggatcc tgagacgcag gccatcgtca tgatcggcga aatcggaggc gatgcggagg
     3961 aaaaagcggc cgagtacatc aagaagcacg tcaagaagcc ggtggtcagc tttattgccg
     4021 gtatcactgc acctcccggc cgccgcatgg gccatgccgg tgcgatcatc tcgggcggca
     4081 aaggcacggc aaacgagaaa atgaaagccc tggaagccgc gggagtccgg gtcatcaaaa
     4141 atcccgccga aatcggcacc actgtcaaga aagcgttggg acgttagaga agcggtaact
     4201 gtaggttcat tcaacttccg tcgccgtatt tgctttccct tcgcctccta tcgtggtagg
     4261 ctctcctaag cctcgcaaga cgtgttgtcc cgcaggtatt cctccgtccg tctaggcctc
     4321 agtcgttgat cgaggccaac ccaggagttt ctttgcgagt atttctgatc cacgttcgcg
     4381 acccgcagtt ttacgcgctt cctgcgaaga cccgcgcgaa aaacggcaac atccgcgtga
     4441 tgggctttcc gcccatcggg atcatgtcgt tgtccgccgt ggtgaaagc
//
LOCUS       DASTQY010000145        12304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_849679, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000145 DASTQY010000000
VERSION     DASTQY010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 12304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12304
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849679"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="VFR82_04635"
     CDS             complement(<1..165)
                     /locus_tag="VFR82_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEU4500917.1"
                     /translation="MLTKRIIPCLDVKDGRVVKGVSFVDLRDAGDPVEVAAVYDREGA
                     DELCFLDITAS"
     gene            complement(192..926)
                     /gene="hisA"
                     /locus_tag="VFR82_04640"
     CDS             complement(192..926)
                     /gene="hisA"
                     /locus_tag="VFR82_04640"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEU4500918.1"
                     /translation="MVVIPAIDVKDGRCVRLRQGDMAAETVYGTDIPSVATRWAHAGA
                     TIIHLVDLNGAVEGEPRNLPQIEAVIQSTPVQVQVGGGIRNMTTVRRYFKSGISRIVL
                     GTGALNDRAFLQQACQEYPRRILLGLDARDGKVAVNGWTSLSDTTATHLLKELAGFEL
                     GAVIYTDIARDGMLSGPNIAALREIMTFSAFPVIASGGISTIEDLQALQSLGPRVEGA
                     IVGKALYDGKLNYREAVAALRGVEGS"
     gene            complement(931..1677)
                     /locus_tag="VFR82_04645"
     CDS             complement(931..1677)
                     /locus_tag="VFR82_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500919.1"
                     /translation="MHSIGEAARSCSTVIIALSLILGSACSGSKVTAQTSSELPRYHI
                     RSIALLPFTTLTTPQVRDAGDMYLSTPQSIQRSDISLGIPSNVEPQLRQTTGVPAHAA
                     EKVTQLFWSRLKDRQGLVILSPTETARVAVKTSHETSQATPEAQAAQLAAKLQADAAL
                     MGKVLVYQERSGSRMGANPPATVGFEVKAVASDGQVLWVGNYYERQKPLTEDFLGFIQ
                     HGGGFVTAEELAQYGTEKVLRTFPFGAGQD"
     gene            complement(1670..2380)
                     /gene="hisH"
                     /locus_tag="VFR82_04650"
     CDS             complement(1670..2380)
                     /gene="hisH"
                     /locus_tag="VFR82_04650"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEU4500920.1"
                     /translation="MGQVSIGEPALLFYEPIAVWLRAWKGGEASGWPMIAIIDYGMGN
                     LRSVWKAFESVGHQAVVTRDPATIKNAGHVVLPGVGAFGDCMANLERFELVEPIRSTV
                     QSGKPFLGICLGLQLLFTESEEFGAHKGLNIIPGHVKRFVPDPALKIPHMGWNQVNVE
                     RPCPLFAGIAAGSHWYFVHSYFVQPVDPMVTATTTTYGTPFVSAIWKDNIVACQFHPE
                     KSQSVGLRLIKNFGAWRA"
     gene            complement(2515..3120)
                     /gene="hisB"
                     /locus_tag="VFR82_04655"
     CDS             complement(2515..3120)
                     /gene="hisB"
                     /locus_tag="VFR82_04655"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEU4500921.1"
                     /translation="MKKDAAPRQASLHRATKETDIRVEWTLDGSGESKIDTGIRFFDH
                     MLELLAKHGFFDLTVQAKGDLDIDEHHTVEDVGIVMGKALHQALGEKAGIKRFGFASA
                     PLDETLAQVTIDLSGRPFLVYNVNLPDRKIKAFDLGLFEDFFQAFVTHGGLNLHVNLM
                     YGRNPHHIMEAIFKATAKALDQATMLEERLAGKVLSTKGTL"
     gene            complement(3117..4400)
                     /gene="hisD"
                     /locus_tag="VFR82_04660"
     CDS             complement(3117..4400)
                     /gene="hisD"
                     /locus_tag="VFR82_04660"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEU4500922.1"
                     /translation="MKIVASTDPAFLPTLKKVAGRSRVQGATAEKPVRAILDAVERDG
                     DRAVLRFTKRFDKVSLAPTQLKISPEEIKESYYKIRKEEGDALRFAAQRIMAFHERQR
                     TKTWMYQDGAATLGQVVTPLDAVGVYVPGGKAVYPSTVLMSAIPARVAGVKRIVMCTP
                     PQKGGINPYLLVAADIAGVTEIYRVGGVQAIGALAFGTKTISKVDKIVGPGNIYVATA
                     KRMLYGTVGIDMVAGPSELLVVADNDAKPAHVAADLLCEAEHDEEAQVWLVTTSASLA
                     KETVSLVERQLKGLQREKIAAKSVRKYSVVFVVTTIDEAIDVANEIAAEHLTLSVDKP
                     FDYLEKIRHAGALFLGRYTPPSVADYIAGPNHVLPTGATARFFSALSIHDYTKISNIV
                     YYTKEELSKVKDHLIRLASIEGFDAHAKSAQSRFT"
     gene            complement(4397..5101)
                     /gene="hisG"
                     /locus_tag="VFR82_04665"
     CDS             complement(4397..5101)
                     /gene="hisG"
                     /locus_tag="VFR82_04665"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEU4500923.1"
                     /translation="MLTIALCKGKLIEPTLKLFAKAGYGAAQPVADSRRLLFPCPEIG
                     MTFLIVRPTDVPTYVEHGAADVGVVGKDVLLEQDSDVYEPLDLGFGACRIAVAAFRGQ
                     VPRDRLSSKIRVATKYPKITERYFNQRGVPVEIIKLYGSIELAPIVGLADRIVDLVET
                     GNTLKAHGLIEVECIARSTARLIVNRASLKLKHRAVAELLSKLRHACATPGPARKDGS
                     LKPARSAGRKPKRMRT"
     gene            complement(5105..6364)
                     /gene="murA"
                     /locus_tag="VFR82_04670"
     CDS             complement(5105..6364)
                     /gene="murA"
                     /locus_tag="VFR82_04670"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU4500924.1"
                     /translation="MDKIVISGGHVLQGEVQISGAKNSALPILASTILGGGECVISNI
                     PRVVDVVTMGKLLGILGAEVHHEGNRAVIKADVIRSTQAPYELVKTMRASVLVLGPLV
                     ARWGEAVVSLPGGCAIGSRPVNLHLAGLAKLGAEVSIEHGYIRAKAKRLKGAPIYCDV
                     PTVTGTENLMMAASLAQGTTVIENAAKEPEIVDLADFLVKRGARIAGAGTDVITIEGV
                     RELRGSDHEVIPDRIEAGTYVVAGAITKGSISVNRCRPEHLEPLLNKLGEAGVAIRVE
                     KERVHIDASKRGRLKGVDVRTLPFPGFPTDMQAQMVALMSVAEGTSIITETVFESRFM
                     HVEELRRMGADIRVEGNRVVITGHERLAGAPVMASDLRASAGLVLAGLAAEGTTDVLR
                     VYHLDRGYERIEEKLGALGAVIERRKG"
     gene            complement(6380..7279)
                     /gene="prmC"
                     /locus_tag="VFR82_04675"
     CDS             complement(6380..7279)
                     /gene="prmC"
                     /locus_tag="VFR82_04675"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU4500925.1"
                     /translation="MSPSVKQRTAQCPTLGFTVLDMRRRLEAAGIDSAEQEALWLIEH
                     ALGLTGLHQIIDRERSLTDGEVARLNDVATRRVAREPLQYILGTQEFCGLEFEVNPTV
                     LIPRPETELLAHEIIRRLPQGTPPTLVDVGTGSGCLAVTLARSIPQARMIATDISTQA
                     LETAMRNARRHAVDSSITWLHGDLLAPLSGLGLEKRTHAILANPPYIRESEWAGLQPE
                     VSRYEPRLALVAGPRGTEVHERLLGEAVSYLMPGGWLAMEVGEGQTMDLCSKIEAMPD
                     YERVEVVPDEAGINRIVIVRRTG"
     gene            complement(7276..8355)
                     /gene="prfA"
                     /locus_tag="VFR82_04680"
     CDS             complement(7276..8355)
                     /gene="prfA"
                     /locus_tag="VFR82_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU4500926.1"
                     /translation="MEAGLLKKWESAANRHDELTTQLMDPSVISQPVLLVKLTKERTE
                     LEGVAQLFRRYQDVLKQLEEAAHLLSDPTGGAELHSLAAEETAQLETEREALEEQVLD
                     HLTPKDPRDDKNLFLEIRAGTGGDEAALFAGDLFRMYVKFAERRKLKVDMVEASETGK
                     GGYKTVIAVIEGKGGYGLFKYESGVHRVQRVPVTEAAGRIHTSTVTVAVMPEVDEVDV
                     QIDPKDLRIDTFCSSGAGGQSVNTTYSAVRITHIPTGVVVSCQDERSQLKNRTKAMRT
                     LRARIVEAERERQEAEIAQTRKAQVGTGDRSEKIRTYNFPQNRVTDHRIGLTLHKLEQ
                     VLEGDMDELVEALRAQRDHAAAEAS"
     gene            complement(8443..8649)
                     /gene="rpmE"
                     /locus_tag="VFR82_04685"
     CDS             complement(8443..8649)
                     /gene="rpmE"
                     /locus_tag="VFR82_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEU4500927.1"
                     /translation="MKKGIHPLYREATVHCACGNSYKTRSTVGDINVDICSSCHPFFT
                     GTQKIVDTEGRVERFKKKYAKKGK"
     gene            complement(8738..9985)
                     /gene="rho"
                     /locus_tag="VFR82_04690"
     CDS             complement(8738..9985)
                     /gene="rho"
                     /locus_tag="VFR82_04690"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEU4500928.1"
                     /translation="MHLADLKQKTIADLNDVARDLKIEGAANLRKQELIFAILQAQTE
                     KNGVVFGEGVLETLPDGFGFLRAPDSNYLPGPDDIYISPSQIRRFNLRTGDIVSGQIR
                     PPKESERYFALLKVEKVNFEDPEVARDKILFDNLTPLYPEERIQLEFDREEYCTRVMD
                     LTTPIGKGQRGLIVAAPRTGKTMLLQAIARAILKNHKEVTLIVLLIDERPEEVTDWQR
                     QVKAEVISSTFDEPAQRHAQVAEMVLEKAKRLVEHKKDVVILLDSITRLARAYNTIAP
                     PSGKVLSGGLDSNALQRPKRFFGAARNIENGGSLTIMATALVDTGSRMDDVIFEEFKG
                     TGNMEVHLDRRLADKRIFPAIDISQSGTRKEELLVDKDRLNKMWILRKVLSPLGTMEA
                     MEFLMDKVHGTKTNQEFLQSMNR"
     gene            10438..10848
                     /locus_tag="VFR82_04695"
     CDS             10438..10848
                     /locus_tag="VFR82_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEU4500929.1"
                     /translation="MAGDEDQEHDRLFTLSEANHLIPQLQSRLASVRQAKAVLARTKD
                     DIRRASARAEYGGGSTAGPLYLSSLHKISSNVQAIQEMGVLVKDLDLGLCDFPHLREG
                     RVVFLCWKLGEQEIKWWHETTSGYKDRCPLEDSV"
     gene            10866..11156
                     /locus_tag="VFR82_04700"
     CDS             10866..11156
                     /locus_tag="VFR82_04700"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU4500930.1"
                     /translation="MKFVIIGFDGPEGEAKRKIYRPAHLANMEPLDQAGRVVLAGPLT
                     DKTGSLIVIEADSLEEAERFAREDPYTVYGVFERVEVHPFTQIFPKSTLPTQ"
     gene            11234..>12304
                     /locus_tag="VFR82_04705"
     CDS             11234..>12304
                     /locus_tag="VFR82_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500931.1"
                     /translation="MRLWGLVALAISTFAISTGNPALAAAKTVIAEAEYVMADGDTLA
                     SAEEKVLQRAQRRAVEEAGVYLESTFYDVEQESYGRTTRRTSLEIRTIAAAITETEIL
                     DSRRSFENERPVFFIRIRANVDLHNLAQAIRRLQSEAQLARHFQELQSENRHLRAQLE
                     DLQQQPSGVRMLSIDASGKPEPVQRAKQQLELAFHAPDLQKKIQLASEAARLDPQSVE
                     ALIVRGQAYLRLISIAYSEHQKPGKYAGHIEQARFDFDQAVELDAKNPWAWTGRGDVQ
                     TWLKRTDEASSAYEHALALDPFFDLARQRLIGLYTTQARRQAKAKQWEQSLLTLHKLL
                     DAPIPDSWRPYQKEAFLLRSDVL"
BASE COUNT         2570 a         3719 c         3294 g         2721 t
ORIGIN      
        1 ggacgccgta atgtcgagaa agcacagctc gtctgcaccc tctcgatcat agaccgccgc
       61 cacttccacc ggatcgccgg catctcgaag atcgacaaag ctcacgcctt tgacaacacg
      121 gccgtctttc acgtcgagac agggaatgat gcgcttggtc aacatagctc atacggcccg
      181 cagacacgtg ctcatgaccc ctcaacaccg cgaagggcgg cgacggcttc gcgatagttc
      241 aactttccgt catacagcgc ctttcccacg attgcgcctt caactcgagg gccgagcgat
      301 tggagagcct gcaggtcttc gattgtggag atcccaccgg aggcgatcac aggaaaagca
      361 gagaacgtca tgatttccct caaggcagcg atgttcggtc cgctcagcat gccatcccgc
      421 gctatgtccg tatatatgac ggcgccaagc tcgaacccgg ccaactcttt gagtagatgc
      481 gttgccgtcg tgtctgacag cgaggtccat ccgtttactg ccaccttgcc gtcgcgagca
      541 tccaagccga gcaagatccg cctgggatat tcttgacaag cctgttgtaa gaacgcgcga
      601 tcattcaatg cccctgtgcc taacacaatg cgggagatgc cggacttgaa atacctccgg
      661 accgttgtca tgttccgaat gccgcccccg acttgaacct ggaccggcgt cgactggatg
      721 acggcctcga tctgcggcag attccgaggt tctccttcta cggccccgtt cagatccacg
      781 aggtgaatga tcgtcgctcc cgcatgtgcc caccgtgttg ccaccgatgg aatgtcggtg
      841 ccatacacgg tttcagcggc catatcgcct tgacgcaacc ggacgcaacg tccatccttc
      901 acatcaatcg ccggtataac gaccatcggt ttagtcttgc cccgcgccga acggaaaggt
      961 tctgagcacc ttctccgtcc catactgggc taattcctcg gcagtcacaa accctccacc
     1021 atgctggata aaccccagaa agtcctctgt cagcggcttc tgccgttcat aataattgcc
     1081 gacccagagc acctgtccgt cggacgccac cgctttcact tcaaagccta ctgtcgcggg
     1141 aggattcgca cccatacggc ttcccgatcg ttcctggtag accaagacct tccccatcag
     1201 tgcagcgtcg gcttgcagct tcgctgccag ctgagctgcc tgtgcttccg gcgtcgcctg
     1261 ggaggtctca tgggaggtct tgaccgcaac ccttgcagtc tcggtgggcg agagtatgac
     1321 gagtccctgc cgatctttca gccgcgacca aaacaactgt gtcaccttct cggccgcatg
     1381 cgccgggacg ccggtcgttt gtcgcaattg cggttcaacg ttggacggga tgcccaagga
     1441 aatatcagag cgttggatac tttgcggcgt tgagagatac atgtctccgg cgtcacggac
     1501 ttgcggagtc gtcaaagtcg taaacggcaa caacgcaatg cttcggatgt gatatcgagg
     1561 cagttcactc gacgtctggg ccgtgacctt ggaaccgctg caggcggagc cgaggatcag
     1621 cgacagggca atgatcacgg tcgagcaact gcgtgctgct tcgccgatgc tatgcacgcc
     1681 aggccccaaa attttttatc aaccgcaagc ccactgattg actcttttct ggatggaact
     1741 gacaggccac gatattgtcc ttccatatgg ccgatacgaa tggagtgccg taggtcgttg
     1801 tggttgctgt gaccattgga tccaccggct ggacgaagta tgaatgcaca aaataccagt
     1861 gagaacccgc ggcgatacct gcaaagagcg gacaaggccg ttcgacgttg acctggttcc
     1921 atcccatgtg tgggatcttc agcgcgggat ccgggacgaa ccgcttcacg tggcccggaa
     1981 tgatattgag ccccttgtgc gcaccgaatt cctcactttc cgtgaacaac agttgaagcc
     2041 ccaagcagat gcccaggaat ggcttacccg attgaacggt ggatcgaatg ggttcaacca
     2101 actcaaatcg ctctaagttg gccatacaat cgccgaaggc gcccacaccg gggaggacga
     2161 catgcccggc atttttgatc gtcgcgggat cacgcgtcac caccgcctgg tggccaaccg
     2221 actcgaaggc tttccagacg ctgcgcaaat tccccatccc gtaatcaata atcgcaatca
     2281 tgggccatcc agaagcctcg cctcccttcc acgctcgtaa ccataccgca atcggctcat
     2341 aaaacagaag ggcaggttcc ccgatggaca cctgccccat tggcactcat tgttctgcga
     2401 tgactagata gcaccggcga cgaagataga gccatgaccc ggctgcaggg gtgatgctgt
     2461 ctcgctctgc tggtcggact gcatgggtat gcgacagata ggacttgcga cgccttacaa
     2521 cgtgcccttg gtcgaaagca cttttcctgc caatcgctct tccaacatcg tcgcctgatc
     2581 aagagctttc gctgtcgctt tgaagatggc ttccatgatg tgatgcgggt tgcgtccata
     2641 catcagattg acatggaggt tcaacccgcc atgggtcacg aaggcctgaa agaaatcttc
     2701 gaagaggcca aggtcgaatg ctttgatctt gcggtccggc aaattgacgt tgtacacgag
     2761 gaatggcctc ccgctgagat cgatggtgac ttgtgccaat gtttcatcca gtggtgccga
     2821 agcgaagccg aatcgcttga tgccggcctt ttcccctagg gcctgatgaa gcgccttgcc
     2881 catgacaatc ccgacatcct ccactgtatg gtgctcgtcg atgtcgagat cacccttggc
     2941 ctgaacagtg agatcaaaaa agccatgctt ggccaacaac tccagcatgt ggtcaaaaaa
     3001 ccgaatgccc gtgtcgatct tgctctctcc gctgccgtcc aacgtccact cgacgcgaat
     3061 gtcggtttcc ttggtcgcac gatgcagcga ggcctgtcta ggggcggcgt cttttttcat
     3121 gtgaacctgc tttgggcgga tttggcatgc gcatcgaagc cttcgatgga cgccagccgg
     3181 ataaggtgat cctttacttt gctcaattct tctttggtgt aatacacaat gttgctgatc
     3241 ttcgtatagt catggatcga cagcgcggaa aaaaaccgcg cagtcgctcc ggtcggcaag
     3301 acgtgattcg gccccgcgat gtagtccgcg acggaaggcg gggtatagcg ccccaagaac
     3361 agtgcgccgg catgtcgaat tttctccaga taatcgaacg gcttgtctac ggacagcgtg
     3421 agatgttcgg ccgcgatctc gttcgccaca tcgatcgctt cgtcgatcgt cgtgaccacg
     3481 aagacgaccg aatatttccg gactgacttg gccgcaattt tctcgcgctg taatcccttc
     3541 agttgtctct cgacaagaga gaccgtctct ttggcaagcg aggcggacgt cgtcactaac
     3601 cagacttgtg cctcctcatc gtgctcagcc tcgcagagca agtcggccgc cacatgcgcg
     3661 ggtttggcat cgttgtccgc gacgaccaac aactcgctcg gtcccgccac catatcaatg
     3721 ccgacagtcc cgtacaacat gcgcttcgcc gtggcaacat agatgttccc cggcccgacg
     3781 atcttgtcga cttttgaaat ggttttcgtg ccgaacgcca gcgcgccgat cgcctgcaca
     3841 ccacccacgc gatagatttc cgtcaccccg gcaatatccg ctgcgacaag caaataggga
     3901 ttaatgccgc ctttctgagg gggtgtgcac atgacgatcc gcttcacacc cgccaccctt
     3961 gctggaatgg cgctcatcaa tacggtggaa ggatagacgg ctttcccgcc cggcacgtac
     4021 acgccgacgg catcaagggg agtgacgacc tgtcccaacg tcgccgctcc atcctgatac
     4081 atccaggttt tggtccgttg ccgctcgtgg aaggccatga tccgttgggc ggcaaaacgc
     4141 aaggcgtcgc cttcctcttt tcgaatcttg tagtagcttt ctttgatttc ttcgggcgag
     4201 atcttcagct gagtcggcgc gagcgacact ttgtcgaatc gcttggtgaa acggagaacc
     4261 gctctatctc catcgcgctc caccgcgtcc aggatagccc tcacgggttt ttcggctgtc
     4321 gccccttgca cccgcgagcg ccccgccacc tttttcaaag tggggaggaa cgccggatct
     4381 gtagaggcaa cgatcttcat gtccgcatcc gcttgggctt ccggcccgca gatcgtgccg
     4441 gctttaacga accgtctttt cgagccggtc caggggtggc gcacgcatgc cggagtttgc
     4501 tgagcaactc ggcgacggcc cggtgtttca atttgagact agcccgattg acgatgaggc
     4561 gtgccgtcga tcgggcgatg cactcgactt cgatgaggcc atgggctttg agcgtgttgc
     4621 ctgtttcaac caggtcgacg atgcgatcgg ccagtccgac aatcggcgcc agttcaatcg
     4681 agccatagag cttgatgatc tcgaccggca cgccgcgttg gttgaaataa cgctctgtaa
     4741 tcttcgggta tttcgtggcg acgcggattt ttgacgagag ccggtcgcgt ggtacttgcc
     4801 ctcgaaaagc ggcgacggca attctacacg ctccgaaccc taaatccaat ggctcgtaga
     4861 cgtcgctgtc ctgttcaagc aacacatctt tgccgacaac acccacgtct gccgcaccgt
     4921 gttccacata ggtgggcaca tcggttggcc ggacgatgag aaacgtcatc ccgatttccg
     4981 ggcaggggaa cagcaagcgg cggctatcag ccacgggctg cgccgcgccg tatcctgcct
     5041 tcgcaaataa cttgagcgtt ggttcgatca atttcccttt gcacaatgca atcgtcagca
     5101 tccgctatcc ttttcgtcgc tcgatgacag caccgagagc cccaagcttc tcttcgattc
     5161 gttcgtagcc ccggtcgaga tgatagacgc gcagcacgtc ggtagtccct tcggccgcca
     5221 ggcccgcgag gaccaatccg gcactggcac gcaagtcgga ggccatgact ggagcgcctg
     5281 caagacgttc gtgccctgtg ataacgacac gattaccttc gacgcgaata tccgccccca
     5341 tacggcgcag ctcctctaca tgcatgaaac ggctttcaaa cacggtctct gtaatgatac
     5401 ttgtgccctc cgccaccgac atgagcgcca ccatctgcgc ctgcatatct gtcgggaaac
     5461 cgggaaaagg caaggttcga acgtcgactc ccttcaatcg tccccgcttc gacgcatcga
     5521 tatggacgcg ttctttttcg acccgaatgg ccacgcccgc ttccccaagc ttgttcaaca
     5581 acggctccag atgttccggc cggcaacggt tcacagaaat cgatcctttt gtaatggccc
     5641 ccgccaccac ataggttccg gcctcgatgc ggtccggaat gacctcatga tcactgcccc
     5701 tcagctcccg gacgccttct atggtgatga cgtcggttcc tgctccggct attcttgctc
     5761 cccgcttgac gagaaagtct gccagatcga cgatctccgg ttccttcgcg gcgttctcaa
     5821 tgacggtggt tccttgagcg agtgaggccg ccatcattaa gttttcagtg cccgtcaccg
     5881 tcggcacatc acaatagatc ggggcacctt tcaggcgttt ggctttggct cgaatgtacc
     5941 cgtgttcgat cgagacttcg gcaccgagct tggccaaccc tgcaagatgc aaattcaccg
     6001 gacgcgaacc gatcgcacaa ccgccgggca aggacacgac ggcttctccc caccgggcaa
     6061 ccagtggacc aagcaccagc acagaggcac gcattgtctt gaccaactca tacggtgcct
     6121 gtgtcgaccg aatgacgtcg gccttgatga ctgcgcggtt cccttcatga tgcacttccg
     6181 cacccaagat cccgagcagc ttccccatcg tgaccacgtc cacgacgcgt ggaatattac
     6241 tgatgacgca ctcgccgccg ccgagaatcg tcgaggccag gatcggcagt gccgagttct
     6301 tggccccgct gatctgaacc tctccctgaa gcacatgccc gccggagatg acgattttgt
     6361 ccatactgtt gcctgcctcc taacctgtcc gccggacgat cacaatccga ttgatacctg
     6421 cctcgtcggg caccacctcc accctttcgt aatccggcat cgcctcgatt tttgaacaga
     6481 ggtccatggt ctgcccctcg cctacttcca tggcaagcca tccgcccggc atcaggtagg
     6541 agactgcttc ccctagcagt cgctcgtgta cttctgttcc tctgggacct gcaacgagtg
     6601 ccagccgagg ctcataacgg ctcacttccg gttggagtcc ggcccactcg gattcacgaa
     6661 tgtaaggagg gttggcgagg atggcatgcg tcctcttctc cagacccaag ccgctcaagg
     6721 gagccaacaa gtctccatgc aaccaggtaa tcgatgagtc gacagcgtga cgacgtgcat
     6781 tgcgcatggc ggtctcgagc gcttgcgttg agatatccgt cgcgatcatc ctcgcctggg
     6841 gaatcgatcg agccagtgtc acggccaaac aaccggatcc agtaccgaca tccacgagcg
     6901 tgggaggcgt gccttgtggc aggcgacgga tgatctcgtg cgcgagcaac tcggtttcag
     6961 gacggggaat gaggacggtc ggattgactt caaactccag cccgcagaat tcctgagtac
     7021 ccagaatgta ttgcaggggc tctcgagcaa cccttcgggt cgcaacgtcg tttaatctgg
     7081 ctacctcacc gtccgtcagc gaacgttccc ggtcaataat ctgatgcaat cccgtgaggc
     7141 ctaacgcatg ctcgatcaac cagagggcct cctgctctgc cgaatcgatt ccggcagcct
     7201 cgagccggcg gcgcatgtcc aggactgtga aaccaagcgt aggacactgc gcagttctct
     7261 gcttgaccga cggactcacg aggcctccgc cgcagcgtga tcacgttggg cgcgcaaggc
     7321 ctcgaccaat tcgtccatat caccttccag cacctgttcc aacttgtgca acgtcaatcc
     7381 gatgcgatgg tccgtgacac ggttctgggg aaagttatac gtgcggattt tttcgctacg
     7441 atccccggtc ccgacctgag ccttacgcgt ttgcgcgatt tcggcctcct gcctttctcg
     7501 ctctgcttcg acgattctgg ctcgaagcgt ccgcatggct tttgtgcggt tcttgagttg
     7561 cgagcgctcg tcctggcagg acaccacgac gccggtggga atatgcgtga tgcgcacggc
     7621 ggagtatgtg gtattcacgc tctgtccgcc ggcgcctgat gaacaaaagg tgtcgatccg
     7681 caggtccttc ggatcgattt gcacatccac ctcatcgact tccggcatga ccgcaaccgt
     7741 gaccgtcgaa gtatggatgc ggcctgccgc ctcggtcacg ggcactcgct gaacacgatg
     7801 gactccgctt tcatacttga acaagccata gccacccttt ccctcgatga cggcgatgac
     7861 ggtcttgtat cctcccttgc cggtttccga ggcctcgacc atgtcgactt tcaacttacg
     7921 ccgttccgca aatttgacat acatccggaa tagatctccg gcaaataagg ccgcctcatc
     7981 cccacccgtt ccggctcgaa tctccaaaaa cagattttta tcgtcccgag gatccttcgg
     8041 cgtcagatga tcaagcacct gttcttcgag cgcctcgcgc tccgtctcca actgagcggt
     8101 ctcctcggct gcaagcgagt ggagttcggc gccgcccgtg ggatccgaca agagatgggc
     8161 ggcctcctcc aattgcttca gaacgtcctg gtaccgccgg aacagctgcg cgaccccttc
     8221 gagttccgtc cgctcttttg tcaacttgac gagcaagacc ggttggctga tcacggacgg
     8281 atccatgagc tgagtggtca gctcgtcatg tcggttggca gccgattccc atttcttcaa
     8341 caggcccgct tccatcacaa tcctcgattg cacgcgatct ggacacaaaa aaagagaccc
     8401 tgcctctgcg aagcagggtc tctcgtatgt tcgtgaaccc gactacttgc ccttcttcgc
     8461 gtatttcttc ttgaaccgtt cgacccgccc ttcggtatcg acgatctttt gcgtgccggt
     8521 aaaaaatgga tggcagctcg agcagatgtc gacgttgatg tccccgacgg tcgatctggt
     8581 cttatagctg ttgccgcagg cgcaatggac tgtcgcctct ctatataatg gatgaatacc
     8641 cttcttcata attccactcc tggcgtccgg gccctctcgc ctccgcttat tcgaaggcgc
     8701 gtcactttaa cctgtgccga ccgaaagata caagcgttca gcggttcatg gattgcaaga
     8761 attcctggtt ggtcttcgtg ccgtgcacct tatccatcag gaattccatg gcctccattg
     8821 tacccagagg gctcaggacc ttgcgtaaaa tccacatctt attcagacgg tccttgtcca
     8881 ccagcaactc ttctttccgg gtgccggact ggctgatgtc gatagccggg aagatacgct
     8941 tgtcggccaa ccggcgatcg agatggacct ccatattgcc ggtcccttta aactcttcaa
     9001 agatcacgtc gtccatgcga ctgccggtgt ccaccagcgc cgtcgccatg atcgtgagac
     9061 ttccgccatt ttcgatattg cgcgcagccc cgaagaaccg ctttggccgt tgcaaggcgt
     9121 ttgagtcgag accgcctgag agcaccttac cgcttggagg agcaatcgtg ttgtaggcgc
     9181 gcgccagacg ggtgatgctg tccaacagga tgacgacgtc cttcttgtgc tcgaccaatc
     9241 gtttggcctt ttcgagtacc atttcggcga cctgagcatg ccgctgagcc ggctcgtcga
     9301 acgtagagct gatgacttcg gctttgacct gccgttgcca gtcggtgacc tcttcaggac
     9361 gttcatcgat caggagaaca atcaacgtca cttctttgtg gttcttcagg atggcccggg
     9421 cgatggcttg gagcagcatc gtcttccccg tgcgaggcgc cgcgacgatc agtccgcgct
     9481 gtccctttcc aataggggtc gtcagatcca tgacacgcgt gcagtattct tctcgatcaa
     9541 actccagttg aatgcgctcc tccggataga gcggggtcag gttatcgaag aggattttgt
     9601 cgcgtgccac ctcagggtct tcgaaattca ccttctcgac cttgagcagc gcaaaatagc
     9661 gctcgctttc cttcggcggt ctgatctggc cggagacgat atcgccggtt ctgagattaa
     9721 accggcgaat ctgcgaggga gatatataga tgtcatcagg acccggcagg taattggagt
     9781 cgggagcgcg caaaaaacca aatccgtccg gcagggtctc gaggacgccc tctccgaaga
     9841 ccaccccatt cttttccgtc tgcgcttgga ggatggcaaa gatcaattcc tgctttcgga
     9901 gattcgcggc cccttcgatt ttcaggtcgc gggcgacgtc gttcaggtcg gcgatggttt
     9961 tctgcttcaa gtcggcaaga tgcataactt ctcccttagt ttgtgccata catatcctca
    10021 agctcggggg taatggacaa agacaagtgt cgccgcggaa atatggtcaa ccattgaaaa
    10081 cgatgcgcaa tgacggttcg atcgtgagtt ggctaattgt gtgttcgcta gaggggatgt
    10141 gctgtgaagg acgtgtccgt tatgatcatc gtggatgcgc ctgcaaagcc gaactcatcc
    10201 ctaagctaat catcggttcg tagagatgcc tcggaagtct tgtgtgtgga gaagacgcag
    10261 actcgacatg aaacgctaac gtggagcata ccctgcaaga cgatcattgt caacaccgag
    10321 ttctttttcg ctcaaagctg tccgtgatga tgagatcaca ggaatccttc cccctccgat
    10381 gccgagcggt tgcagggctt ttgaatggta tgttacacca cacagaagga gttgacgatg
    10441 gctggagacg aggatcaaga acatgaccgg ctcttcacgc tgtcggaggc caatcactta
    10501 atcccacagt tacagtcccg tcttgcatcc gttcgtcagg ccaaagcggt cctggcacgg
    10561 accaaggacg acatccgtcg tgccagtgca cgggcggagt atggtggtgg aagcacggcc
    10621 ggcccactct acctgtccag cctgcataaa atcagctcga atgttcaagc catccaggag
    10681 atgggagtgc tggtcaagga cctcgacctc ggactctgcg acttccccca cctccgggaa
    10741 ggccgggtcg tgtttctctg ctggaagctc ggtgagcagg aaataaaatg gtggcatgaa
    10801 acgaccagcg gatacaaaga ccgttgccca ttggaagact cagtctgaat gttggcctgt
    10861 cagcgatgaa attcgtcatc atcggctttg atggccccga gggagaagcc aaacgaaaga
    10921 tttaccgtcc agctcaccta gccaatatgg aaccgctcga ccaagcagga cgagtcgtac
    10981 tcgcaggccc gcttacggat aagactggca gccttattgt catcgaggcc gattctttgg
    11041 aagaggccga gcggtttgcc cgcgaagatc cgtacaccgt ctacggcgta ttcgaacggg
    11101 tcgaagtcca tcctttcacc cagatctttc ccaaatcgac gttgccgacc cagtaattca
    11161 gcgggttacg atctacccgc gccccccggc agtcgcgttg ccgagccaat gggccagcgg
    11221 tatagttaga catatgagat tatggggttt ggtggctctc gccatatcga cgttcgcaat
    11281 ctctacaggt aatcctgccc ttgccgccgc aaaaacagtc attgcggaag ccgaatacgt
    11341 catggctgat ggcgacacac tggcgagcgc ggaagaaaaa gtcctacaac gcgctcagcg
    11401 gagagcggta gaagaggccg gtgtctatct ggaatccact ttctacgacg tcgagcagga
    11461 atcctatgga cgcaccactc gacgcacctc gttggagatc cgcacgatcg ccgcggccat
    11521 caccgagacg gagattctcg attcacgccg ctccttcgag aacgaacgcc ctgtcttttt
    11581 catacgcatc cgtgccaacg tggatttaca caatttggcc caagccattc ggcgcctcca
    11641 gtcagaggcc cagctggcaa ggcattttca agaactgcag tcggagaacc ggcatctgcg
    11701 cgcgcaactc gaagacttgc agcaacagcc gtcgggcgtg cgcatgctga gcattgatgc
    11761 gagcgggaag cctgaaccgg ttcagcgagc caagcagcaa ctcgagctcg cttttcatgc
    11821 tccggacttg caaaagaaga ttcagttggc ctcggaggcc gcgcgactgg atccccaatc
    11881 cgtcgaggcc ttgatcgtga gaggccaggc ctatttgcgg ctgatctcaa tcgcctattc
    11941 agaacaccag aagcccggca agtatgcggg gcatatcgag caagcgagat ttgatttcga
    12001 tcaagcggtg gagctggacg ccaagaatcc ctgggcgtgg accggaagag gcgatgtgca
    12061 aacctggctt aagcgcaccg atgaggcctc gtcggcttat gagcatgcgc tggccttaga
    12121 cccttttttt gatctcgcga ggcagcgcct catcggcctc tatacgacgc aggcacgccg
    12181 acaggccaaa gccaaacaat gggagcagtc acttctgact ctccacaaac tgctcgacgc
    12241 accgattcca gacagctggc gaccctacca gaaagaagcc tttcttcttc gcagcgacgt
    12301 cctg
//
LOCUS       DASTQY010000146         4018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1496604, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000146 DASTQY010000000
VERSION     DASTQY010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496604"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2168
                     /locus_tag="VFR82_04710"
     CDS             <1..2168
                     /locus_tag="VFR82_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4500932.1"
                     /translation="FLIPCLALTFYYGQVVVPGGEDPDSFFPTTSYALVLLLINLDLI
                     GFVVLLLLLSRNVIKAYFERRHRLFGSGFRTKLVAAFIGFSLIPTVLLAFVASGLVNK
                     AVDVWFSDQIDHVMRDSYEVAKMQHAGHITLAVNSARAISHEIFREDMLLPEQRDLLV
                     AAMARKRVEFGVAGIEVYSAKMETLTKSLDAEVPAGVLDLPIGQLVLQAINGKQETNT
                     VQEAATGRLVRAATPVAASGRGNEVGGVVVVDAYVPESLLAKMDGIGRQYEEYKQIKA
                     MKNPIKAGAYLFVAVITVLILFGASWFGFYVARSITVPIQRLAEATDAIAQGDLSVRI
                     DAKATDEIGTLIDSFNRMTADLQSSKSTIESANVSLRRTNVELDRRRAYIETVVDTIA
                     AGLLSIDKNGVVTNFNPSGERILGLSAGRVQGRPANEAFKEFGLTLFQTIYDRMLQDQ
                     RDDLTLDGTIEVEGKFLTVALHASRMKDEQEQDLGVVLVFEDLTELLKAQKVAAWQEV
                     ARRVAHEIKNPLTPIQLSAQRMRKKFMEKAPDFDRVFDESTNVIVNEVASLKHMVDEF
                     SKFARLPAPQLVLQSLHDVINEVISLYRGGHKDIELIVHLDEDLPHLKFDWEQIKRVL
                     VNLLDNAIQAMNQKGRVWITTQYDTRRRRAVVSIADEGNGIAIEDQEKLFVPYFTRKK
                     TGTGLGLAIVRRIITDHDGHISAGNNQPRGAVFTFELPV"
     gene            2187..3590
                     /locus_tag="VFR82_04715"
     CDS             2187..3590
                     /locus_tag="VFR82_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU4500933.1"
                     /translation="MSASILVVDDEEAIVSSLGSILQDEGYEIAVAKSGVEALKIYTV
                     DPPDLMLLDIWMPEMDGLETLRRVKELVPTAQVMVMSGHGSIETAVKAIKLGAYDYIE
                     KPLSLENVTLRVKHALEQFRLEEENRSLRTKVQRKFELVGQSPAMQQLRQLIDTAGPT
                     NSRVLVGGENGTGKELVARAIHLQSARADRPFVAVNCAAIPETLIESELFGHEKGSFS
                     GATSMKRGQFEQADGGTLFLDEIGDMSLNTQAKVLRALQEQQFNRVGGTKLLKVDVRV
                     LAASNKDLMKEIEKGTFREDLYYRLNVVPIVVPPLRERRDDIPLLIRHFMKLHAEEQG
                     LRTKEVTPEAMAVFQQYEWPGNIRELRNLIERLMIMVPGNVIDAAQANLTLQVKAPGA
                     VAVATPAVQMATSPLFTQPFDSLRDARNAFEKEYIGRKLREHHWNISRTAEDLKIERS
                     HLHRKIKLLDVEMRPEA"
     gene            3665..>4018
                     /locus_tag="VFR82_04720"
     CDS             3665..>4018
                     /locus_tag="VFR82_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEU4500934.1"
                     /translation="MTTYREAGVDIDAGDEFVDRIKPLVRSTFRPEVLADLGGFGGLF
                     RLQAQQYADPVLVSGTDGVGTKLKIAFMMDRHDTVGIDLVAMCVNDIVVSGAEPLFFL
                     DYFATGKLAVDKAQQV"
BASE COUNT          870 a         1101 c         1191 g          856 t
ORIGIN      
        1 tgttcctcat tccttgtctg gcgctgacct tctattacgg acaggtcgtg gtgcccggcg
       61 gcgaagatcc cgattccttt ttcccgacca ccagctatgc gcttgtcctc ctactgatca
      121 acctcgatct gatcggcttc gttgtgctct tgctcctcct ttctcgaaac gtcatcaagg
      181 cctacttcga gcggcgtcat cggttgtttg gttctggctt tcgcacgaag cttgtggccg
      241 cctttatcgg attttcgctc atcccgacgg tccttttggc tttcgtggcc agcggcctcg
      301 tgaataaggc ggtggacgtc tggttcagcg atcaaatcga ccatgtcatg cgtgactcct
      361 acgaagtcgc caagatgcag catgccggcc acattacgct tgctgttaac agcgcccgtg
      421 ccatcagcca cgaaattttc agggaagata tgttgctgcc cgagcagcgc gatctcttgg
      481 tcgcggccat ggcgcgcaag cgggtggaat tcggtgtggc cggtatcgaa gtgtactccg
      541 ccaagatgga aacgctgacg aagtcgctcg atgcggaggt gcccgcaggc gtcctcgatt
      601 tgccgatcgg ccagctcgtc ctgcaggcga tcaatgggaa gcaggaaacg aacaccgtac
      661 aggaggccgc gaccggcagg ctggtccgcg ccgcaactcc ggtggcggca agcgggcggg
      721 gaaacgaagt cgggggggtg gtagtggtcg acgcctacgt ccccgagtcg ctgttggcca
      781 aaatggacgg catcgggcgg cagtatgaag agtataagca gatcaaggcg atgaagaacc
      841 ccatcaaagc cggcgcctat ttgttcgtcg ccgtgattac ggtgctgatt ctgtttggag
      901 cctcgtggtt cgggttctac gtggcgcgca gcatcacggt tccgatccag cgattggcgg
      961 aagccacgga tgcgatcgcc cagggcgatc tctcggtgcg catcgacgcc aaagcgaccg
     1021 atgagatcgg caccctgatc gattcgttca accgcatgac cgccgacttg caaagcagca
     1081 agtccacgat cgaatccgcc aacgtgtcct tacgccggac caatgtcgag ttggaccggc
     1141 gccgtgcgta tatcgaaacc gtcgtcgaca cgattgccgc cggactgctc tcgatcgaca
     1201 agaacggggt ggtgaccaat ttcaatccat ccggcgagag aattctcggc ctgtccgcag
     1261 gccgcgtcca gggacgtccg gcgaacgaag ccttcaagga attcggtctg acgctgtttc
     1321 agactattta cgatcgcatg cttcaggatc agcgtgatga tctgacgctc gacggtacga
     1381 ttgaagtcga aggaaagttc ctgaccgtcg cgctgcatgc gtccagaatg aaagatgaac
     1441 aggaacagga tctcggcgta gtcctggtgt ttgaagactt gaccgaacta ctcaaggcac
     1501 agaaggttgc ggcttggcag gaagtggcgc gccgggtcgc gcatgaaatc aaaaatcctc
     1561 tcacgcccat ccagctatcc gcccagcgaa tgcggaaaaa attcatggag aaagccccgg
     1621 acttcgatcg cgtatttgac gagtcgacca atgtcatcgt gaacgaggtc gccagtctca
     1681 agcacatggt cgatgagttc tcgaagtttg ccagattacc ggctcctcag ttggtgctgc
     1741 agtcgttgca tgacgtgatc aacgaagtga tctccttgta tcgcgggggt cataaagaca
     1801 tcgaattgat cgtgcatctc gacgaggact tgccgcactt gaagtttgat tgggaacaga
     1861 tcaagcgtgt gttggtcaat ttgctggaca atgccatcca ggccatgaac caaaaaggac
     1921 gggtctggat cacgacccaa tatgacacca gacggcgtcg cgccgtggtg tctattgccg
     1981 atgagggcaa cggcatcgcg atcgaggatc aggagaagct gtttgtgccc tatttcacca
     2041 ggaagaaaac aggaaccggc ttggggctgg cgattgtccg ccgcatcatt acggatcatg
     2101 acggccacat ttcggccggc aacaatcagc cgcgcggcgc tgtgtttacc tttgaattgc
     2161 cggtgtaggg gaggtgtctc aagcctatgt cagcgtcgat cttggttgta gatgatgagg
     2221 aagcgatcgt gtcgtcgctg ggcagtattc tccaggacga gggctatgag attgccgtgg
     2281 ccaagagcgg tgtcgaagcg ctgaagatct atacggtcga tccccccgat ctcatgttgc
     2341 tggatatttg gatgcctgaa atggacggcc tggaaacctt gcgccgcgtc aaagaactcg
     2401 tgccgaccgc tcaggtcatg gtgatgtccg gccatggctc catcgaaacg gcggtcaaag
     2461 cgattaagct gggggcctat gactatatcg agaagccgct gtccctggaa aatgtcaccc
     2521 tgcgggtcaa gcacgcgtta gagcaattcc ggctggagga agaaaatcgg tcgctccgca
     2581 ccaaggtgca gcgaaaattt gaactcgtcg ggcaatctcc agccatgcag cagttgcgtc
     2641 aattgatcga tacggcgggg ccgaccaaca gccgcgtgct cgtcggaggc gagaacggga
     2701 ccggcaagga attggtggcc cgtgcgattc atcttcagag cgctcgagcg gaccgtccgt
     2761 tcgtggccgt caattgcgcc gcgattcccg aaaccctcat tgagagcgaa ctcttcgggc
     2821 acgaaaaggg atcgtttagc ggcgccacgt caatgaaacg tggacaattc gagcaggccg
     2881 acggggggac gctctttttg gatgaaatcg gcgacatgag tttgaatact caggccaagg
     2941 tgctccgtgc gcttcaggaa caacaattca accgagtcgg cgggaccaag ttgttgaagg
     3001 tcgatgtccg cgtgctggcc gcctcaaaca aagatctcat gaaagagatt gaaaaaggca
     3061 cgttccggga agacctctat taccggttga acgttgtccc gatcgtcgtg cccccgcttc
     3121 gggaacgccg ggacgatatt ccgctgctca tccgccattt catgaagcta catgccgaag
     3181 agcagggctt gcggacaaaa gaggtgacgc ccgaagccat ggctgtcttt cagcaatatg
     3241 agtggccggg caacattcgg gagcttcgaa acttgatcga gcgcctgatg atcatggtcc
     3301 ccggcaacgt gatcgacgcg gcccaggcca acctgacact tcaagtcaaa gctccgggag
     3361 ccgttgctgt tgcaactcct gcggtccaga tggcgaccag ccctttgttt acccagccgt
     3421 ttgactcgtt gcgagatgcc cgcaacgcgt ttgaaaagga atatatcggg cgaaaattgc
     3481 gggaacatca ttggaacatt tctcgcacgg ccgaggatct caagatcgag cgcagccacc
     3541 tgcatcgaaa gatcaagctg ctcgatgtgg aaatgcggcc ggaagcctga gcgcgcgggc
     3601 tctcaccctg cttctcatcg cgttgacctc ttcaagactg ctccgctaag atgcgtgccc
     3661 tgctatgacc acctatcgcg aagccggtgt ggacatcgac gccggagacg agttcgtcga
     3721 tcgcatcaag ccacttgtcc gttcgacttt ccgcccggaa gtgttggcag acttgggtgg
     3781 attcggggga ctcttccgcc tgcaagccca acagtatgca gatccggttc tcgtctccgg
     3841 caccgatggc gtcggcacaa agctgaaaat cgcctttatg atggaccggc acgacacggt
     3901 gggcatcgat ctcgtggcga tgtgcgtcaa tgacattgtg gtgagtggag ccgagccctt
     3961 gttcttcctg gactatttcg ccacggggaa gctcgccgtt gacaaagcgc aacaggtt
//
LOCUS       DASTQY010000147         4102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1252193, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000147 DASTQY010000000
VERSION     DASTQY010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252193"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2249
                     /gene="dnaE"
                     /locus_tag="VFR82_04725"
     CDS             <1..2249
                     /gene="dnaE"
                     /locus_tag="VFR82_04725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU4500935.1"
                     /translation="KRIEKELAVIRQKSFAHYFLVVEEIVRQAPRTCGRGSVAASIVS
                     YCLQITHVDPLKHHLFFERFLNPGRKDPPDIDIDFPWDERDQILTWVFQHYGDRQAAM
                     VANQNSLGFRAAIRETAKVYGISAAEITAIASRITRQTGLLQFADVPTNGQWANRLSQ
                     ALRLQAPWPEILAQALHAQNHFRHLSMHCGGLVIVPDEIRRYVPVEFTAKGLPVIQWE
                     KDQTEEAGLVKIDLLGNRSLAVIRDAIAAVACHTGHTIDYASWDPLTDEATRDLIRRG
                     DTIGCFYIESPATRLLLKKLWKGMPADRLAVADVFDYLVMVSSLVRPAAITFVNDFVT
                     RAHGHSHDPLHPLMESILEETHGIMVYQEDVTKVAMALGNFSLEDADQLRKVISKKHK
                     HRQLRDYYHQFCRGAAKNGASSATIAKIWTMIMSFAGYSFCKPHSASYAQVSFKSAYL
                     RAHYPAEFMAAVISNEGGFYSTFAYLSEARRMGLAILPPDVNTSDWAYHGEGDRLRMG
                     LMQVKTIPKPVATAIVAERDQAGPFRSFQDFLRRVPLEPMHARALIRAGCCDSISGEL
                     TRPALLWRVFAGKEAGPLPVPDEYPAFQKRGHEIEAFGFLASRHPLTLYRKQIEQLRP
                     LPASQMHQFIGCRITTVGWLITEKPVETKHGLPMEFITLEDHTALYDATLFPDVYRRR
                     CHVLSSNRAYVVQGLVEEAFGVATLTVLDLYPLDSTTAVAWPHENTWYDGLCDAPPDQ
                     PTHSSPLT"
     gene            2201..2839
                     /locus_tag="VFR82_04730"
     CDS             2201..2839
                     /locus_tag="VFR82_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEU4500936.1"
                     /translation="MRRPARSADSFIPSHLSLPTLGEAAARCTGCDLYKQATQTVFGE
                     GAAHASILLVGEQPGDQEDRAGHPFVGPAGKILDKALDEAGIDRRDLYVTNAVKHFKW
                     EPQGKRRKHKKPSVAEIAACRPWLEAELQVVGPRVVVCLGATAAQSVFAKAVRLGEMR
                     GRAWATPLAAAVFVTIHPSAILRHPESAQREQEYRRFVQDLHYITSYLRKAA"
     gene            2847..3419
                     /locus_tag="VFR82_04735"
     CDS             2847..3419
                     /locus_tag="VFR82_04735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEU4500937.1"
                     /translation="MPRSHRSAKSGLLIIDMINEYRFDDADKLFPAIEQTATHIANLK
                     RQMKARSLPVVYVNDNFGKWQSDFRKLVTRCLHEQCRGKTITQILLPDEDDYFVLKPK
                     HSGFYATPLEILLRFLQVERVIITGVAGDNCVLFTAADAYMREFEVTVPSDCVISIDA
                     NANRSALNHMRVTLKAKIAPSSALLTHLAA"
     gene            complement(3545..>4102)
                     /locus_tag="VFR82_04740"
     CDS             complement(3545..>4102)
                     /locus_tag="VFR82_04740"
                     /inference="COORDINATES: protein motif:HMM:NF017847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEU4500938.1"
                     /translation="GNLTYTPEERLILNDCQLLAAKPVLFVANVSEGKNADEETVKKV
                     RDFAQKRGARVVSICGQLEAELASLPESERPDFLNEMGLTESGLVRLTREAYTLLDLI
                     TFFTAGETESRAWPIPRDTKAPQAAGKIHSDMERGFIRAEVYDYDDLLACGSEAKVKE
                     KGLFRLEGKEYVIKEADIVYFRFNV"
BASE COUNT          887 a         1199 c         1126 g          890 t
ORIGIN      
        1 gcaaacgaat cgaaaaggaa ttggccgtca tccggcaaaa aagtttcgcc cattattttc
       61 ttgtggtgga agaaatcgtc cgtcaggctc cgcgcacttg cggccgggga tcggttgcgg
      121 cttcgattgt ctcgtactgt ctccagatca cgcatgtcga cccgctcaag caccatttgt
      181 tctttgaacg gtttctcaat ccaggccgga aagatccacc cgacatcgac atcgattttc
      241 cgtgggacga gcgggatcaa atcttgacgt gggtattcca acactatggc gaccgtcagg
      301 ctgccatggt ggcgaatcag aacagtctgg gcttccgcgc agccatccgc gaaacggcga
      361 aagtctatgg gatctcagcc gcggaaatca cggcgatcgc ttcacgcatc acacggcaaa
      421 caggtctcct gcaatttgcc gacgtgccga ccaacgggca atgggcgaac cgactctcgc
      481 aggcgctgcg actccaagcg ccatggcccg agatactcgc ccaagccctt catgcacaga
      541 atcattttcg ccacctctca atgcattgcg gcggcctggt gattgttccg gacgaaatta
      601 ggcgatacgt gccggtcgag ttcacagcaa aaggcctacc ggtgattcaa tgggaaaagg
      661 atcagacgga ggaagcgggg ctcgtcaaaa ttgatttgct gggcaaccgc tcgcttgcgg
      721 tcatccggga tgcgattgcc gccgtggcgt gccacaccgg gcataccatt gactatgcga
      781 gttgggatcc gctgacggac gaagccaccc gcgatctgat ccgacgcgga gataccatcg
      841 gctgtttcta tattgaatct cccgcaacaa ggctcttgct caaaaagctc tggaagggga
      901 tgcctgccga tcgtcttgcc gtggccgacg tcttcgacta tctcgtgatg gtctcttcgc
      961 tggtccggcc cgccgcgatc accttcgtga acgacttcgt gacgcgcgcg cacgggcact
     1021 cacatgatcc tcttcacccc ttgatggaat ccatcctgga agaaacccat ggcatcatgg
     1081 tctaccagga agacgtcacg aaagtggcga tggcgctcgg gaatttttca ttggaagacg
     1141 cggaccaact gcggaaggtg atcagcaaaa aacacaaaca ccggcagctg cgggactatt
     1201 atcatcagtt ttgccgcggt gcagcgaaga acggcgcctc ttcggccacg atcgccaaaa
     1261 tctggacaat gatcatgagc tttgccggct acagtttttg caagcctcat tcggccagct
     1321 atgctcaggt ctcgttcaag tccgcctatc tgcgagctca ttacccggcc gaattcatgg
     1381 ccgctgtcat cagcaatgag ggcggttttt attcgacctt cgcctatctt tcggaagcca
     1441 ggcggatggg actggcgatc ctccctcccg atgtaaatac gagcgattgg gcttatcacg
     1501 gcgaaggaga ccgacttcga atgggcttga tgcaggtaaa aacgattccc aaacccgttg
     1561 ccacggcgat cgtggccgaa cgtgaccagg caggtccgtt ccgatcgttt caggattttc
     1621 tccggcgcgt gccccttgag cccatgcatg cccgggcgct gatccgcgcc ggctgctgcg
     1681 attccatctc cggcgagttg acacgaccgg ctctgctgtg gcgggtgttc gctgggaaag
     1741 aagccggtcc cttacctgtc ccggacgagt atccggcttt ccaaaagcgc ggtcatgaga
     1801 tcgaggcgtt cggctttctc gccagccgcc atcccttgac actgtatcgt aagcagattg
     1861 agcaactgcg tccgttgccg gcctcgcaga tgcatcagtt catcggatgc cgcatcacca
     1921 cggtgggatg gctcatcacc gaaaagccgg ttgaaaccaa acacggcctg ccgatggaat
     1981 tcatcacgct tgaagatcac acggcactgt atgacgccac tcttttcccc gatgtctatc
     2041 ggcggcgttg ccatgtgctt tcatccaacc gggcctatgt cgtccaggga cttgtagagg
     2101 aagccttcgg tgtcgcgacc ctcactgtgc ttgatctgta cccgctggat tccacgaccg
     2161 ccgtcgcatg gccgcatgag aacacgtggt atgatggcct atgcgacgcc ccgcccgatc
     2221 agccgactca ttcatcccct ctcacctaag tcttcccaca ttaggagagg ctgctgcccg
     2281 ctgcacgggg tgcgacttgt acaagcaggc cacccagacc gtgtttggag aaggcgcggc
     2341 acatgcttcg atccttctgg ttggcgagca accgggggat caagaggacc gcgcaggtca
     2401 tccctttgta ggaccggcgg gaaaaatttt agataaggca ctagacgaag ccggaattga
     2461 ccgtcgggac ctgtatgtca cgaatgccgt aaaacacttt aaatgggaac cgcagggcaa
     2521 gcgccgcaaa cacaagaagc catctgtagc tgagatcgcc gcctgccggc cctggctgga
     2581 agccgaattg caggtagtgg ggccgcgcgt cgtcgtctgc ctcggcgcga ccgcagctca
     2641 atcagtcttc gccaaggccg tccgactggg tgaaatgcga ggtcgggcgt gggcgacccc
     2701 gctcgccgcc gccgtctttg ttacgatcca cccttctgct atccttcgac atcccgaatc
     2761 ggcgcagcgc gagcaagaat accgccgttt cgtgcaggac ttgcactaca tcacgtcgta
     2821 cctgcggaag gccgcctaaa cagatcatgc cgcgctcaca tcgctctgcc aagtctggcc
     2881 tactcattat cgatatgatc aatgagtatc gtttcgatga cgcagacaag ctgtttcccg
     2941 ccatcgaaca aactgcaacg catatcgcga acctgaaacg gcagatgaaa gcgcgcagcc
     3001 taccggtcgt ctacgtaaac gataatttcg gcaagtggca gtcggacttt cgtaaacttg
     3061 tgactcgatg tctgcatgag caatgccgtg ggaagaccat cacccagatt ctgctgccag
     3121 atgaagacga ttactttgta ttgaaaccga agcactcagg cttttatgcg acgccgctcg
     3181 agatactgct tcgctttctg caagttgagc gagtgattat tacgggagtg gccggcgaca
     3241 attgcgtctt gttcaccgcg gccgatgcct acatgcgaga gtttgaagtg accgtgcctt
     3301 cggactgtgt tatctcgatc gatgccaacg cgaatcgctc ggccctgaac cacatgcgag
     3361 taaccctcaa agcaaagatc gccccttcga gcgcattatt gacacacctt gcggcgtaac
     3421 atatctgctg ggtgcgcggc gtttctgaga caggtgtcgc cggcacacga atgttcgaca
     3481 tcgggaacgg gtggatgtaa tagtgacgca agaacgcggg acacagaggg gacgtggcgg
     3541 tcaatcacac gttgaatcgg aaatacacaa tgtcagcttc ttttatgaca tattccttcc
     3601 cttccagtcg aaacaaccct ttttctttca ccttggcttc tgatccgcat gccagcagat
     3661 catcgtagtc gtagacctcg gcacggatga agccgcgctc catgtcagaa tgaatcttgc
     3721 cggctgcctg aggagccttg gtatctctcg gaatgggcca ggcgcgggat tcggtctcgc
     3781 cagccgtgaa gaatgtgatc agatcgagca gggtgtaagc ttcccgtgtc aggcgcacca
     3841 gaccggactc ggtcagtccc atctcattca agaagtccgg tcgttcactc tccggcaagg
     3901 aggcgagctc ggcctccaac tgaccgcaga ttgacacaac acgggcgcca cgtttctgtg
     3961 cgaagtcgcg aaccttcttg acggtctctt catcggcatt cttgccttct gacacattgg
     4021 ccacaaagag caccggcttg gctgcaagca gttgacagtc gttgaggatg agccgctcct
     4081 ccggtgtata ggtcagattt cc
//
LOCUS       DASTQY010000148         5158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_445237, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000148 DASTQY010000000
VERSION     DASTQY010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445237"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="VFR82_04745"
     CDS             complement(<1..1240)
                     /locus_tag="VFR82_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU4500939.1"
                     /translation="MEGSHVSVSADGGILSVEADRKKAGRSLKSRPSSIEQNVLDLLV
                     CRGIVSQAVLDRAAHEAAEGGDDMESLLLDRFQVPKDVLGAAMSDYYQCPYLPYDERT
                     VIASDLLRPLNSDYLKKHLWLPVARRGQLLDVLAHDPHDLERGWDVRRSFPGMTIRYS
                     VGLRRDIEQFLGATTEQQGSASTIGTILNELEEDLPLDPVHERPSGPIDENDSLIVRL
                     TNHLISEADRLGASDLHFEPSTGKREMNIRFRVDGTCFSYTKVPAAYRRALISRLKIM
                     ANLDIAERRKPQDGKIRFKLDAQRSIELRVATLPTGGGNEDAVLRLLTAKEPMPLEAM
                     ELTPSALQAIKAMAEQPHGIILCVGPTGSGKTTLLHAILKHINTEERKIWTAEDPVEI
                     TQEGLRQLQIHQKIGLDFATA"
     gene            complement(1253..2716)
                     /locus_tag="VFR82_04750"
     CDS             complement(1253..2716)
                     /locus_tag="VFR82_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500940.1"
                     /translation="MKIRRDRLVENRGHVLLASLMLVIMLSLLSMTALYLVGQDAPGI
                     SAMREQGQSQQLADAATELVVSWFHDASTTPTSITDLLAKRRADATGASSFFDAEGRS
                     QFTGTPDHPDLLLESVNEDDDRILNRAPSGFSGPLSETGRLEMIKLYAPVEPGLLGTV
                     EVTASTRGSRPAASTIRFQLGAVHLPPVRAAMQTGQGLTASSPEAASPFLVHWGDTRV
                     MGDMAITRMEDLVVKSTTASINGQSYETLGAFEDRWVDYWIGGNLSVLSTSGGTMSAP
                     QNVHTRQEPTPGTRLDRWDYDLLKKTAQRYGTYYRLDRDGRLHELGSIESDPGRSPSE
                     VLASLTVGQSHGLLFIDTLDGETPRSDNLGTLVVDTDYVEALLVVQGHILVRPSGPGR
                     SVTVLSPPPEGTSALGGRIPVTLPGIHLNGMLYAAGNITVERQTRIFGAVMTSGTVAG
                     GTRPPALEIWYNFDFSKGLFRGLPVVYRAPSTWQPKF"
     gene            complement(2736..3482)
                     /locus_tag="VFR82_04755"
     CDS             complement(2736..3482)
                     /locus_tag="VFR82_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500941.1"
                     /translation="MQTRSAARKSRVRVPLFTQSGSSLVEVLAAMSAGLVVLGAALQS
                     FSYFHQQFLKEGNTIIQAQDVRVGLELLAQELHLASIGSLSVARPDAVEFTANISGLM
                     TKVTAPGVMGETTLAVEDGRGWPDNKLIGVCWNERCDSFTLARAGHRSLLAFVEPIAR
                     PIPAGASAAVKNRVRYYSRLDESGILRFLRQVDGGASVLVGHVESIMFSYWDAQGRKT
                     AHPGLVRRVIVEITARHRAVASKREITLRT"
     gene            complement(3497..3988)
                     /locus_tag="VFR82_04760"
     CDS             complement(3497..3988)
                     /locus_tag="VFR82_04760"
                     /inference="COORDINATES: protein motif:HMM:NF041940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor X domain-containing protein"
                     /protein_id="HEU4500942.1"
                     /translation="MTDWSPSQSIGGATFSEVLVAMALTSVGLVATMGAFQAAHQQFG
                     RGVLATRAMEMAESRLEAKRAVRWDQLLTEDLDQDGLPESFMHDDGTAGDRVAGDGIY
                     SARYEQNGVTLIWTVAPNQGGNLALSGYVLIEARASYRSHYGDHELRVVTLRANAVLA
                     GHH"
     gene            complement(3985..4452)
                     /locus_tag="VFR82_04765"
     CDS             complement(3985..4452)
                     /locus_tag="VFR82_04765"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEU4500943.1"
                     /translation="MSHWKYGAIARLPASSGRTLLELLVVLAIAGIMASMAGPAFQSL
                     NARLQARTATAEIASTLRMARQLAMARRERLLMRFDLSGKTISLQRAEADGILNVYRY
                     GDKGIVVDEPTAGCDLLFHPSGRSATASTIIIHDRDNRRTTITVSLSGRVVIS"
     gene            complement(4696..>5158)
                     /locus_tag="VFR82_04770"
     CDS             complement(4696..>5158)
                     /locus_tag="VFR82_04770"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEU4500944.1"
                     /translation="FSALLVVTGTDLTHRIIPNVVTVPGIVIGLLGAATVLPVGLMNA
                     LLGVAIGGGILWALAWASPYLFGKEGMGGGDIKLLAMIGAFLGWKPALLTIMVGSLSG
                     SLIGLSLIGLGLMKRDEYIPFGPFLVFGALVSMFFAQPLLEWYQTLLTPSG"
BASE COUNT         1135 a         1551 c         1411 g         1061 t
ORIGIN      
        1 tggccgtcgc aaaatccaag ccgattttct ggtgaatttg tagttgccgc aaaccttctt
       61 gcgtgatctc gacggggtct tcagcggtcc aaattttgcg ttcttccgtg ttgatgtgct
      121 tgagaatagc atgcagcagc gtcgttttcc ccgagccggt cggccctacg cacaggataa
      181 tgccgtgcgg ctgttccgcc atcgccttga tcgcctgcaa tgcgctcgga gtcaactcca
      241 tagcttccag aggcatcggt tccttcgcgg tcaaaaggcg cagcacggca tcttcgttgc
      301 cgccgccggt tggcagggtg gccacacgca attcaatgct tcgttgggcg tcgagcttga
      361 atcggatctt cccatcttgc ggcttacgcc gttcggcaat gtcgaggttc gccatgatct
      421 tgaggcggga gatcaatgcc cggcggtaag cggctggcac tttcgtgtaa ctaaagcagg
      481 tcccatcgac ccggaaccgg atgttcattt cccgcttgcc cgtgctcggc tcaaaatgca
      541 gatcagacgc accaagccga tccgcttcgc taatcaaatg gttcgtgagc cggacgataa
      601 gggaatcgtt ctcgtcgata gggccggaag gcctctcgtg caccggatca agaggaagat
      661 cttcctccaa ttcatttaag atggttccga tggttgaagc gctcccctgc tgctccgtcg
      721 tggcgccaag gaactgttca atgtcgcggc gaagcccaac ggaataccgg atggtcatgc
      781 ccggaaaact gcgccggacg tcccaccctc gttcaagatc gtgaggatcg tgagccagca
      841 cgtcgagcag ttggccgcgg cgagcaacgg gcaaccaaag gtgttttttc aagtagtcag
      901 aattgagtgg gcgcagtaga tccgaagcaa tcactgtccg ttcgtcgtaa ggaaggtacg
      961 ggcattggta atagtcactc atcgccgcgc ccagcacatc tttgggaacc tgaaaacgat
     1021 ccaagagcag gctttccata tcgtcaccgc cttcggccgc ctcgtgtgcc gcacggtcca
     1081 acacggcttg agagacaatg ccccggcaaa cgagcagatc caggacgttc tgttcaatcg
     1141 aggaaggacg gcttttcaaa cttcttccgg ccttttttct atccgcctca accgagagga
     1201 taccgccatc ggctgagact gagacgtgag aaccctccat cggatcctcg catcaaaatt
     1261 tcggttgcca tgtggaagga gcacgataga cgacaggcaa gccgcggaag agtcctttac
     1321 tgaaatcaaa gttgtaccaa atttctaaag caggaggccg agttccaccg gcgaccgtcc
     1381 ctgaggtcat aacagcgccg aaaatgcgag tctgtcgttc caccgtgatg ttgcctgccg
     1441 catataacat gccattgagg tgaatccctg gcagtgttac gggaattctg ccgcccaagg
     1501 cactcgtccc ttcaggaggc ggactcaaca ccgtcaccga gcgccccggt ccgcttggcc
     1561 tcacgaggat atggccttgc acgaccagca gagcttcgac atagtctgta tcaacgacca
     1621 acgtacctag attgtccgat cgcggggtct ctccgtccaa agtatcgatg aacagaaggc
     1681 catgcgattg tccgaccgtc aatgaggcca acacctcgga aggcgaccgg ccgggatccg
     1741 actcgatcga tcccaactca tgaagtcgtc catctcgatc gagccgataa taggttccat
     1801 agcgctgcgc cgttttcttc agcaagtcgt agtcccaccg atcaagccgt gttccgggcg
     1861 tcggttcttg gcgcgtgtgc acattctggg gagcactcat agttcctcca gaagtcgaaa
     1921 gaacggagag attgccgccg atccagtagt ccacccaccg gtcttcaaac gcccccagcg
     1981 tctcgtagga ttgaccgttg attgaggcgg tcgtgctttt gaccacaaga tcttccatgc
     2041 gggtgatcgc catatcgccc atcacccgcg tatcgcccca atgcactagg aaagggctcg
     2101 ctgcctccgg cgaagacgcg gtcaatcctt gcccggtctg catcgccgct ctcactggcg
     2161 ggagatgcac ggcgcccaac tggaaccgaa ttgtgctggc ggcaggcctg ctgcctcggg
     2221 ttgaagccgt gacttccacc gtgccaagta gacccggttc cactggcgca tacaatttga
     2281 tcatctcgag acgtcccgtc tccgataagg ggccgctgaa tccacttggc gcccgattga
     2341 gtatgcgatc atcgtcctca ttgaccgact ccaagagaag atccgggtgg tcaggggttc
     2401 cagtgaactg ggaacgaccc tcggcatcaa aaaaggagga agcgcccgtt gcatcagctc
     2461 gccgcttggc gagcaagtcg gtaatggacg tcggcgtcgt cgacgcgtcg tgaaaccagc
     2521 tgacgaccag ctcggtggcg gcgtcagcca attgctggga ctggccttgc tctctcatgg
     2581 ctgaaatccc cggcgcgtcc tgtcccacca ggtacaacgc ggtcatgctc aacagcgaca
     2641 acatgatcac cagcatcagg gaagccagaa gcacatggcc acgattctca accaggcggt
     2701 cccgtcgtat cttcatagtc acctcactca gcggttcatg ttcgcaacgt gatttctcgc
     2761 ttgcttgcca ctgcacggtg tcgagcggta atttcgacaa tcacccgtcg cacaaggcct
     2821 ggatgcgcgg tcttccgacc ttgtgcatcc caatacgaga acatgatcga ctcgacatgt
     2881 cccaccagga cgctggcgcc gccatcaacc tgccgcagga atctcaagat cccgctctcg
     2941 tccagtcggc tatagtagcg cacccgattt ttcaccgcgg cggaagcacc ggctggaatg
     3001 ggtcgagcaa tcggctcgac aaatgccagc aggctccgat gtcctgctcg ggcaagcgtg
     3061 aatgaatcgc agcgttcgtt ccagcagacc ccaatgagct tgttatccgg ccagccgcga
     3121 ccgtcctcaa cagccagagt cgtttctccc atcacaccag gtgcggtgac cttcgtcatg
     3181 agtccgctga tattggccgt aaactcgaca gcatcagggc gagcgaccga tagagagccg
     3241 atgctcgcca aatggagttc ttgtgcgagc aactcgaggc ccacccggac gtcctgagct
     3301 tggataatag tattcccctc cttgaggaac tgctggtgaa agtaggagaa agattgcaac
     3361 gccgcgccta agacaacgag ccccgctgac attgctgcga ggacttccac tagactggat
     3421 ccggactggg taaagagcgg caccctcacc cgtgattttc tcgccgcgct ccttgtttgc
     3481 atgtgcagga tctccgttag tgatgtccgg ctaacaccgc gttggcgcga agagttacga
     3541 cccgcagttc gtgatcgcca tagtgggacc ggtagcttgc acgggcctcg atgagcacat
     3601 acccagagag ggcgaggttt cccccctgat tcggagccac tgtccaaatc agcgtgactc
     3661 cgttctgttc gtacctggcc gaataaatgc cgtcgccggc cacgcgatct cctgcagtgc
     3721 catcatcgtg catgaatgac tccggtaggc catcctgatc gagatcttcc gtcagcaact
     3781 ggtcccaacg aaccgcccgc ttcgcttcaa gtctcgattc cgccatttcc atcgctctgg
     3841 ttgcaaggac cccgcggccg aattgttgat gtgcggcctg aaatgctccc atcgtcgcta
     3901 ccaatccgac ggaggtcagg gccatggcca ccagcacctc agaaaacgtg gcgccaccga
     3961 tagattgaga tggcgaccag tcggtcatga gatcaccact cgcccgctca agctgacggt
     4021 gatcgtggtg cgtcgattgt cccggtcgtg gataataatg gtgctggcgg ttgccgagcg
     4081 tccgctagga tgaaagagga ggtcgcaccc cgctgtcggt tcgtcaacga cgataccctt
     4141 gtccccatat cggtagacat tcagaattcc atcggcctcc gctcgctgga gtgagatcgt
     4201 tttccctgaa aggtcgaatc gcatgaggag gcgctcccgc cttgccatgg ccagttgcct
     4261 ggccattctc aaggtcgacg cgatttcagc agtcgccgtc ctcgcctgta ggcgagcatt
     4321 gagagactgg aacgccggtc ccgccatgga ggccatgatt ccagcaatcg ctaacacgac
     4381 aagaagttcg aggagagtcc tccccgatga cgctggaagt cgcgcaatcg ctccatactt
     4441 ccaatgactc attccaggct ccgttgtttg gcatttctga ttccaagact tgtgccgcac
     4501 acgtcgtata ggcactcgac agcatgtcca cagcaggtat catgcagttc agcgcacttg
     4561 tctctcggag actcttgaaa gattgcaatg aaaaacgccg cgagttggat acaggcagga
     4621 ggtgaatcag atacatgccg gccaggcaca tccggccgcg gaacgcacag cgagtctgac
     4681 aaggctttgg atcagctatc cggaaggagt cagcaaggtc tgataccact cgagcagcgg
     4741 ctgggcaaaa aacatggaga caagggcacc aaatacgagg aatgggccga aggggatgta
     4801 ttcatcgcgt ttcatcaatc caaggccgat caggctcagg cctataagag aaccagacaa
     4861 cgaaccgacc ataatcgtca agagcgccgg tttccagccc aagaatgcgc cgatcatggc
     4921 gagtaacttg atgtcaccgc cgcccatccc ctccttgcca aacagatatg ggctagccca
     4981 ggccaaggcc caaagaattc ccccgccgat cgcgaccccc aacaaggcgt tcatcaaacc
     5041 aaccggcagc accgtggctg cgccaagcag gccgatgacg atgccgggaa cggtcacgac
     5101 attcggaatg attctgtgag tcaggtcagt tcctgtcacc acgagcaacg ccgagaac
//
LOCUS       DASTQY010000149         9902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_623748, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000149 DASTQY010000000
VERSION     DASTQY010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9902
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623748"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..1512
                     /locus_tag="VFR82_04775"
     CDS             28..1512
                     /locus_tag="VFR82_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HEU4500945.1"
                     /translation="MLKRIFGLETEYGLLVHQDRPDHSPTWFAHQIRDHIFHVQRQGV
                     LDLHHRGHDEPPGNGGFLTNAGRIYLDMGHLEYASPECHSLTDLVASDRAGDTILQRA
                     VHDLGLRDQVSLIRNNVDHETDATFGSHENYLVTRRFPFSRRGLAPLVTFLVTRQIYT
                     GSGRVGAASPQDAWIQMDRLIVPRAALGHRSQSPQLPFQISQRADHIVNDFFEWVQQN
                     RAIVNTRDEPLADPNQYRRIHLLLGDSNMAEYATALKLGTTGLVLQLIEEGKSPQNLD
                     LDEPVEALQEISQDQDREWILRLQSGKRMSAIDIQEQFLAAAKTHYAGQDDETAWVLE
                     QWETVLQDLRGDYSCLVGRVDWASKLWLLETFREAENLEWNDPMLKSLDLEYHNLNAE
                     KGLYYGLLQEGRVPRLTTDKAVELAMEHPPRNTRAFGRGELVKHLLTCGPPAELEAAR
                     KEERLFPSYVINWSIFQLRGLDPFPMPDPFKSYAQEVHSHLVRP"
     gene            1635..2093
                     /locus_tag="VFR82_04780"
     CDS             1635..2093
                     /locus_tag="VFR82_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500946.1"
                     /translation="MKAKRWMMEYGVAMMLAMLFAVVLGHLPLFRDTAVGKLHASDMV
                     QFIGYGAGIVLAWFGARQLAADPPEEWKWLVAYRSLILPVTTLIVVGLAYHVLLYVCQ
                     PFIGKSGKETYNWTFIIGLIVCCGWLIFTWVQKCAPQIASAEPRRLRKAA"
     gene            complement(2138..3169)
                     /locus_tag="VFR82_04785"
     CDS             complement(2138..3169)
                     /locus_tag="VFR82_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU4500947.1"
                     /translation="MTEGRCQRTVTYAGDAEAMALPAFVPARPLRNHHLMTVVPRYWS
                     RGPLLAGIPVERRLFQTEPDTQIVGFCHWQDNRRAAQTMLLLHGLEGCCDSHYMHGIA
                     AKAYKAGLNVVRLNQRTCGGTEHLSPTLYNSGLSADYRSVLHELAAEQAHRIWLVGYS
                     MGGNLLLKATGELGRSQPALAGCVVVCPNIDPTLCVHELERPANWLYHRHFLTRLKAR
                     LRRKALLSPDKWDSKGLGAITRISEFDDRYTAPDGGYRDAADYYDRSGARHVLHSIAV
                     PTTIITAQDDPFIPYSMFLTPAVQQNSSIRLLAPTHGGHCGFFQTRRSEEDRYWVENR
                     IVEIVRRRE"
     gene            complement(3179..4384)
                     /locus_tag="VFR82_04790"
     CDS             complement(3179..4384)
                     /locus_tag="VFR82_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU4500948.1"
                     /translation="MNPPPLHLPDILLAITLLLTISAVMIPLARRVGIGPELGLLLSG
                     VVLGLPHLPAPAHVARLRELSELGVVFFLFVIGLELDLKQAWSLRRYALGMGVLQVAV
                     TGVVLMFYWLWFSQSWSLGLLLGLVLANSSTALVIQILERKNELDQEHGRAAFAVLLF
                     QDLTVVPIIALVPLLAGTESTDVHSLWQVLPAVGAVALLFFLGRKICPWLFSMASEKH
                     MSETFTAILFIAVLGSAWVASRMGLSMALGAFLMGVALSGTQQRHQLRNEVMPFKNLL
                     LGLFFVAVGLTVDVSVLIEHPVRILMHVLVIVVVKVAVLYLAARIIRMEHGPAARVAF
                     LLAQAGEFGFVILGTLLASSVITPTQFGNGIMVIALTTIMTPLLDTMGMAWSRLNRSV
                     MPGAKASTS"
     gene            complement(4605..4680)
                     /locus_tag="VFR82_04795"
     tRNA            complement(4605..4680)
                     /locus_tag="VFR82_04795"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4645..4647),aa:Asn,seq:gtt)
     gene            complement(4744..5016)
                     /locus_tag="VFR82_04800"
     CDS             complement(4744..5016)
                     /locus_tag="VFR82_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500949.1"
                     /translation="MAHDEDNVIEELLKEISGLISEYPKAIERRAAVIQATGKDPELV
                     EKLVKAADTMRDSGNLYLTWAKHYAALAQGNTDASSDEDETEDFDV"
     gene            complement(5091..5369)
                     /locus_tag="VFR82_04805"
     CDS             complement(5091..5369)
                     /locus_tag="VFR82_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HEU4500950.1"
                     /translation="MYNVAQVIDDKCTAKKGCRLCIMYCPEANCLDLNSAKMVAEVNI
                     DRCKGCELCVVVCNAAKHEAILMQAVSATGQLMSKKGESAALGQAYQG"
     gene            complement(5388..6095)
                     /locus_tag="VFR82_04810"
     CDS             complement(5388..6095)
                     /locus_tag="VFR82_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEU4500951.1"
                     /translation="MIKKRLNIRMSGLGGQGAVTAAHVLAMAANRDGKFSISNPFFGA
                     EKRMAPAESYCRIGIERIYDRGELVFPDVIQVFHPQVITMGKSYTMPFYSGVKEGGVV
                     VINSAQPLLSEEDVQRLKDLNVAVFYIAGTELAIEVAGTELSTNMAMIGSVSGITKCV
                     TMEALDGALQERFGKKFVASGGTASLDEAIKKKFAKKEMLLAKNLATVKRAYEIASEW
                     AEKNKVELQVGNPAVAA"
     gene            complement(6113..7015)
                     /locus_tag="VFR82_04815"
     CDS             complement(6113..7015)
                     /locus_tag="VFR82_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU4500952.1"
                     /translation="MSKERIKISEDLYDIMPSDYQDLVKSATYGKEDRGWKDIGSSKE
                     LIEQHSLCAGCPESMAFRYILASLPNPEDTVMVGSTGCTSLVFPMVAVHNIHSLFGNQ
                     NAIASGLKRALSVRFPGRVKDVVVLAGDGATVDIGLDMTLQAWFRQEKFTTICFDNEL
                     YANTGGQESGLMQKGFVAKMAPVGKLFDKVRLPEIARESGCHYVVNCTVSKPSLVEKV
                     IRNAVHIAREIGPTYLQLYTPCILEIGKNSMEGLQEMRDSEKPAERFAYKEYVSEPAK
                     QLLAELAAKDKERKAAAKQLAGQA"
     gene            complement(7067..8278)
                     /locus_tag="VFR82_04820"
     CDS             complement(7067..8278)
                     /locus_tag="VFR82_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU4500953.1"
                     /translation="MADTHSVIGTQNKKGQTFTDTWKMMHEAPRTPSFFTGSEVIKEA
                     LRRASCDVMIAYPITPQSEAAALIGELFAEGYIGDYFRGESEFAVMSQCAGAAFGGAR
                     VFTTTAGPGTMRAMENFPMWAGARLPIQMIVTCRGINSPLSIQPDTLEIAYLLNTGML
                     VWHAETAQDFFDWILKGYMVSEEPDVHLPLALCCDGFFVTHTKDVVNLTPADMCLPPY
                     DPYRSPVPCMDMECPPVRMMRDPFVMKSNYISYATHASWQQEIWAAIERSRKHSIHWL
                     NGLIDTEDIDQEIMIVASGTAVSQGREAIRLLEDEGVRCGLIKIKTLRPWPEEEIREA
                     TKNAKHIFVPEFNVTGWLAKEIRATIPNPHRVHAGPHVCGGMTMPPEIIVSEIKTTLG
                     MKSFSLAGRGS"
     gene            complement(8319..8954)
                     /locus_tag="VFR82_04825"
     CDS             complement(8319..8954)
                     /locus_tag="VFR82_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HEU4500954.1"
                     /translation="MSQYRVLPGPEHFLPPAAASMGIFLPNPGEAHINGVIVSEEKAY
                     EEAARQFLMAKVPTIFPGPLVLWAWNEKAAKKATAIRHLYNTLKECVQPGQTAMLIPM
                     PDYRPKYPKINPEVEINPNHPNLTIWHNKIDCCMFVGVHCHQANLSLKIIRGGTSCYT
                     IAMCAQAGHEDAMLSFRDASVEKIMKLADTVKRLKGTVQPRQTSAKSGASN"
     gene            complement(9294..>9902)
                     /locus_tag="VFR82_04830"
     CDS             complement(9294..>9902)
                     /locus_tag="VFR82_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500955.1"
                     /translation="PLIVRGQAYLRLISIAYSEHQKSGEYAGHIEKARSDFDQAVELD
                     VKNPWAWIGKGDVQTWLKRTDEASLAYERALALDPFFDLARQRLIGLYTTQARRQAKA
                     KQWQQSLLTLHKLLDAPVPDSWRPYQKEAFLLRSDVLLKLHRPEQALDDLSTVILIDP
                     THVAALVTRAHLYQARLQGRLAKDDFERACVLGSIKACNQLP"
BASE COUNT         2119 a         2782 c         2796 g         2205 t
ORIGIN      
        1 aggacatcaa gagctcactg taacgccatg ctgaaacgaa ttttcgggtt ggagacggag
       61 tacggactcc tcgttcatca agaccgacct gaccactcac ccacctggtt cgcgcaccaa
      121 atccgtgatc atatcttcca cgttcagcgg cagggcgtgc tggatctgca tcatcgtggg
      181 cacgacgagc cgccggggaa cggcggtttc ttgacgaatg ccggacggat ttatctcgac
      241 atggggcatt tggagtatgc ctcgccggaa tgccattcgc tgaccgacct cgtggccagc
      301 gatcgagccg gagataccat tttgcaacgg gccgtgcacg atctcgggct ccgcgatcag
      361 gtctcgctaa tcagaaacaa cgtcgaccat gagaccgacg ccacttttgg ctcccacgaa
      421 aactacttgg tcactcgccg attcccgttc agccggcgtg gtctcgcgcc gctggtgacg
      481 tttcttgtga caaggcagat ttacaccggt tcaggtcgag tgggggccgc aagcccccag
      541 gatgcctgga ttcaaatgga tcggctgatc gtgccgcgcg cggcgctagg ccatcggtcg
      601 caatcccccc agctcccgtt tcaaatttca caacgggcgg accacatcgt caatgatttc
      661 ttcgagtggg tgcagcagaa ccgcgcgatc gtgaacacgc gtgacgagcc cttggccgat
      721 ccgaaccagt accgccgcat ccatctcttg ctgggcgatt cgaatatggc cgagtatgcg
      781 acggcgttga aactcggaac gaccggattg gtgctgcaac tgatcgaaga aggcaaaagc
      841 ccgcagaatc ttgatctcga cgaacccgtc gaggcgttgc aagagatctc ccaggaccag
      901 gatcgcgaat ggatcttgcg cctgcagtcc gggaagcgca tgtccgccat tgacattcag
      961 gagcagtttc tggctgccgc aaagacgcat tacgcagggc aggacgacga aacagcctgg
     1021 gtactcgaac aatgggaaac cgtcttgcaa gatctccgtg gcgattacag ctgcctggtg
     1081 ggacgtgtcg actgggcgtc gaaattgtgg ttgttggaga ccttccggga ggcggaaaac
     1141 ctggagtgga acgatccaat gctgaagagc ttggatctgg agtaccacaa tctcaacgct
     1201 gagaaaggcc tgtactatgg attactgcaa gaagggcggg tgccgcgctt gacgacggac
     1261 aaggccgtgg agttggcgat ggagcaccca ccccgcaaca cccgcgcttt cggccggggc
     1321 gagcttgtca agcacttgct gacctgtggg ccaccggcag aattggaggc tgcacggaag
     1381 gaagaacggt tgttcccgtc ttatgtcatc aattggtcga ttttccaact acgcgggctc
     1441 gacccgtttc ccatgccgga tccttttaag agttatgcgc aggaagtcca ctcccatctc
     1501 gtcaggccct aagccggcca gtcaactgcc ctccgatcgc ttcaatccta aggtcttgat
     1561 tcgcctcggg aaaatcgtcc ggtgctatgc ttatagctgg cttatcgcgt tggaaatacg
     1621 tgaggaggtt cagcatgaaa gcaaagcgat ggatgatgga atacggagtg gccatgatgt
     1681 tggcgatgct ctttgctgtt gtcttagggc atttgcccct cttccgggac actgcggtgg
     1741 gaaaactgca tgcgtctgac atggttcaat tcattggtta tggcgccggc atcgtgttgg
     1801 cctggttcgg agcacggcaa cttgcggctg atccgcccga agagtggaag tggttggtag
     1861 cctatcgatc actgatcttg ccggtgacga ctctcatcgt cgtggggctc gcctatcatg
     1921 tcctgttgta tgtctgtcag ccctttatcg gtaaatcggg caaggagaca tataactgga
     1981 cattcattat cgggctcatc gtctgctgcg gctggttgat ttttacctgg gtccaaaagt
     2041 gcgcgccaca gatcgcatcg gcggaacccc ggcggctgag aaaggccgca taagttattg
     2101 cgtgtccggc tgcttcatcc agaggcagcc ggtttttcta ttcccgccgt cttacaatct
     2161 cgacgattcg gttttccacc cagtaccggt cttcctcact tcgcctcgtc tggaaaaatc
     2221 cgcaatggcc gccatgagtg ggagcgagca aacggatgga tgaattttgc tgtacggcag
     2281 gagtcaggaa catggagtag ggaataaagg gatcatcttg cgcggtgatg atcgtcgtag
     2341 ggaccgcaat gctgtggagg acgtgacggg cacctgaacg atcgtagtaa tctgccgcat
     2401 cacgataccc gccgtcgggg gccgtgtaac ggtcgtcgaa ttcactgatg cgcgtaatgg
     2461 cccccaggcc tttcgaatcc cacttgtccg gagacagcaa cgccttccgc cgcaggcgcg
     2521 ccttgagcct tgtcaggaag tgacggtggt acagccagtt ggccggccgt tccaattcat
     2581 ggacacacaa ggtcgggtcg atgttcggac acacgacgac acagcccgca agtgccggtt
     2641 ggctccggcc cagttcacct gttgccttga ggagcaggtt accgcccata gaatatccca
     2701 cgagccagat gcgatgcgcc tgctcggcgg ccaactcatg cagcacgctg cggtaatctg
     2761 cactgaggcc gctgttgtac agggtaggag acagatgttc cgtgcctcca caggtgcgtt
     2821 gattgaggcg aaccacattc aggcccgcct tgtaggcttt cgctgcaatg ccatgcatgt
     2881 aatgtgaatc gcaacagcct tcgagcccat gcaggagaag catcgtttga gctgcccgcc
     2941 gattgtcctg ccagtggcag aatccgacga tctgcgtatc aggctccgtc tgaaaaagcc
     3001 gccgctcaac cgggataccg gccaacagtg gtcccctcga ccagtagcga ggcacgacgg
     3061 tcatgagatg atgattccga agcgggcgag cgggaacgaa tgccggcaat gccatcgcct
     3121 cggcatcgcc agcgtaagtg accgttctct ggcaccttcc ttcggtcaca tcactccctc
     3181 aagacgttga agccttcgct cccggcatga ccgatcgatt gaggcggctc caggccattc
     3241 ccatggtatc gagcaacggc gtcatgatgg tggtcagtgc gatcaccatg atcccattgc
     3301 caaactgggt gggagtgatg acgctggaag cgagtagggt tcccagaatg acgaagccga
     3361 attctccggc ctgtgcgaga aggaaggcaa cacgcgcagc cggtccgtgt tccatccgga
     3421 tgatccgggc cgcgaggtac agcacggcaa ccttcacgac cacgattacg agaacatgca
     3481 taaggattct gacgggatgt tcgattaaaa cgctcacatc gactgtgagg cccacggcga
     3541 caaagaagag tcctagcaac aggttcttga aaggcatgac ttcatttctg agctggtgcc
     3601 gctgttgtgt gccggacaag gcgactccca tcaggaacgc gcctagcgcc atcgacaacc
     3661 ccatgcgtga ggcgacccag gcgctgccca acacggcgat gaaaagaatc gccgtgaagg
     3721 tttcgctcat gtgtttctcg cttgccatgc tgaacaacca cggacagatc ttccggccaa
     3781 ggaagaaaag caatgctact gcgccgactg ccggcaatac ctgccataat gaatggacgt
     3841 ccgtcgattc cgttcctgcc agcagaggca caagcgcgat gatgggcaca acggtcaaat
     3901 cctggaacaa gagaacggca aacgcggccc ggccatgctc ctggtcgagc tcattcttcc
     3961 tttccagaat ttgaatgacc agcgccgttg aggagtttgc caacacgagt ccgagcaata
     4021 gtcctaagga ccagctttgg ctgaaccaga gccaatagaa catcagcacg acgcctgtca
     4081 ctgccacttg cagcactccc attcccagag cgtatcgccg taacgaccag gcttgtttga
     4141 ggtccagctc gaggccgatg acaaacagga aaaacaccac gcccaattct gagagctccc
     4201 gcaagcgtgc cacatgggcg ggggcaggaa gatgcggaag tccgagcacc actcccgaca
     4261 gcaacaagcc gagctcaggc ccgataccca cgcgccgggc aagaggaatc atgacggcgc
     4321 tgatcgtcaa taatagggtg atggccaaca ggatgtctgg taggtgtagg ggaggcgggt
     4381 tcaacgtgtt ttccccacgc gttctgttcc attcatcact cgttttcgat ttgagtagag
     4441 catattcgtc ttcctgcacg gacgtgcgaa cagaatgctt ctgacttcag ccgatgtcac
     4501 accgtttggc gatgagacgc gacgggggct atgccgaaaa gacaaacaca agaagaaggt
     4561 cattttctcg caggagcaag ctcggagcga tcatgtggtt agactggctc cccgggcagg
     4621 actcgaacct gcgaccagcc ggttaacagc cggctgctct accaactgag ctaccgggga
     4681 acgaggttca atgcgaagag ggagtcattc taacaaacta ctcgaagaag ggggagccct
     4741 tttttagacg tcaaaatctt ctgtctcgtc ctcgtcagaa gaggcatctg tgttgccctg
     4801 cgccagggca gcataatgct tagcccacgt gagatacaga ttgccgctgt cccgcattgt
     4861 gtccgctgcc ttgaccagtt tttcaacgag ctctggatcc ttgccggtcg cttggatgac
     4921 ggcggcacgg cgctcgatgg ccttgggata ctccgagata agaccggaaa tctccttcag
     4981 cagttcctca ataacgttat cttcatcgtg tgccataccg tacctcgtat accaacaaaa
     5041 aaccccatag cgcgaacgcc atggggttgt tcagtgccat gtgggcccgt ctacccctgg
     5101 taggcttgtc cgagagctgc cgattctccc tttttcgaca tcaactggcc ggtcgcactc
     5161 acagcctgca tcaagatagc ttcgtgcttg gcagcgttgc agactaccac gcacaactca
     5221 cagcctttgc agcgatcgat gttgacttcc gccaccatct tggctgagtt gagatcgagg
     5281 cagttcgcct cgggacagta catgatgcag aggcgacagc ctttcttggc tgtacatttg
     5341 tcgtcgataa cttgcgcgac gttatacatt aatgttcgtt ccctttctta cgcggcaacg
     5401 gccggattgc cgacctgcaa ctcaaccttg ttcttttcgg cccattcact tgcaatctcg
     5461 taggcacgct tcactgtcgc aaggttcttg gcgagcagca tttctttctt cgcgaatttc
     5521 ttcttgatcg cttcgtcgag tgaagcggta ccgcccgacg cgacaaactt tttcccgaac
     5581 cgctcttgaa gggctccatc gagggcttcc atcgtgacgc atttggtgat gcctgatacc
     5641 gacccgatca tggccatgtt tgtagataac tcggtgccgg cgacttcaat ggcgagctca
     5701 gtacctgcaa tgtaaaacac cgcaacattc aaatccttca gccgttggac gtcctcttct
     5761 gagagcagag gttgagcgga gttgatcacg acgacaccgc cctccttgac gccggaataa
     5821 aacggcatcg tatagctctt ccccatggtg atgacctgcg ggtggaacac ttggatcacg
     5881 tccggaaaca ccaattcgcc gcggtcgtaa atccgttcaa tgccaatgcg gcagtagctc
     5941 tcggccggcg ccatccgttt ttcggccccg aagaacggat tggaaatgga aaacttgccg
     6001 tcccggttgg cggccatggc caacacatgc gccgcggtga cggcgccttg cccgcccaag
     6061 cctgacattc ggatattgag tcgctttttg atcatgatgg cctccggttc cgttaggctt
     6121 ggccagccaa ctgcttggcg gccgcttttc gctccttgtc cttcgcggca agttcggcca
     6181 acaactgttt cgccggctcg ctgacgtatt ccttgtacgc gaaccgctca gctggcttct
     6241 ccgaatcccg catttcctgc agcccttcca tgctgttctt gccgatttcg agaatgcacg
     6301 gtgtatagag ctgaagatag gtggggccga tttcccgcgc gatgtggacg gcattgcgga
     6361 tgaccttctc gaccagcgac ggcttgctga ccgtgcagtt gacgacataa tgacaaccgg
     6421 actcgcgggc gatctccggc agccggactt tgtcgaacag cttgccgacg ggcgccatct
     6481 tggcgacgaa gcccttttgc atcagtccgc tctcctggcc gcctgtgttg gcatagagct
     6541 cattgtcgaa acagatcgta gtgaacttct cctggcggaa ccaggcttgg agcgtcatgt
     6601 ccagaccaat atcgacggta gcgccgtcac cggccaagac gaccacatcc ttgacgcggc
     6661 cggggaaccg aacgctcagg gctcgtttca ggcccgaggc gatggcgttc tgattgccga
     6721 acagtgagtg gatgttgtga acggcgacca ttgggaacac caggctggtg cagccggttg
     6781 aaccgaccat cacggtgtct tcgggattcg gcagtgaggc aaggatatac cgaaaggcca
     6841 tggactccgg acagccggca cacaacgaat gctgttcgat cagttccttc gacgaaccga
     6901 tatccttcca gccacggtct tccttgccgt aggtcgcgct tttcacgaga tcttggtagt
     6961 ccgacggcat gatgtcgtaa aggtcttcag aaatcttgat ccgttccttg ctcatgaggc
     7021 ctcctcagat tcgctcgcgg gagcgaacag ttcaaatttg tgctgctcag cttccacgac
     7081 cggccaggga gaagctcttc atcccgaggg tcgtcttgat ttccgatacg atgatttccg
     7141 gcggcatggt catgcctccg cacacatggg gaccggcgtg tacacgatgc gggtttggaa
     7201 tcgtcgcccg gatttccttc gccaaccaac cggtcacgtt gaattcgggc acgaaaatgt
     7261 gcttggcgtt cttggtggct tcacggatct cttcttccgg ccagggccgt agcgttttga
     7321 tcttgatcag accacaacgc acgccctcgt cctccagaag tcggatggct tctcgtcctt
     7381 gcgacaccgc cgtgcccgag gccacgatca taatttcttg gtcgatgtcc tcggtatcga
     7441 tcaggccgtt gagccaatgg atcgaatgtt tgcgcgagcg ttcaatcgcc gcccatattt
     7501 cctgctgcca ggaggcatgg gtcgcatagc tgatgtaatt gcttttcatg acgaacggat
     7561 cacgcatcat gcgaaccggc ggacattcca tgtccatgca gggcaccggt gagcggtagg
     7621 gatcataggg cggcaggcac atgtcggctg gcgtgagatt caccacgtcc ttcgtatgcg
     7681 tgacaaagaa gccatcgcaa cagagggcca gcggcagatg cacatccggt tcttccgaca
     7741 ccatgtagcc cttcagaatc caatcgaaga agtcctgagc tgtttctgcg tgccagacca
     7801 acatcccggt attgagtaga taggcgattt ccagcgtgtc aggttgaatc gacagcggcg
     7861 agttgatgcc gcgacaggtg acgatcattt ggatgggcaa acgcgcaccg gcccacatgg
     7921 ggaagttctc catggcgcgc atcgtgcctg gacctgccgt ggtcgtgaac acgcgtgccc
     7981 ctccgaacgc cgcaccggca cactgcgaca tgacggcaaa ctcactttcc ccccggaaat
     8041 aatcaccgat atatccctcg gcaaacaact caccgatgag cgctgcagct tcgctttgcg
     8101 gcgtgatcgg atacgcaatc atgacgtcac agctggcccg ccgaagcgct tccttgatga
     8161 cctcgctgcc tgtaaagaac gacggagtgc gcggcgcttc gtgcatcatt ttccaggtat
     8221 cagtaaatgt ctgtcctttt ttattttggg taccgataac tgaatgggtg tctgccatgg
     8281 accagcctcc tttcactgcg cacggtgctt caaacggctt agttcgaagc cccgcttttg
     8341 gccgatgtct gccgtggttg aacagtacct tttaagcgtt tgactgtgtc cgccagtttc
     8401 atgatttttt cgacagatgc atcacggaat gagagcatcg catcttcgtg cccggcttgt
     8461 gcgcacatcg cgatggtgta acaggaagtg ccgccgcgga tgatcttgag tgacagattg
     8521 gcttggtgac aatgcacacc gacgaacatg cagcaatcga tcttgttgtg ccaaatagtc
     8581 aggttggggt ggttcggatt gatttcgacc tcaggattga tcttgggata tttcggtcga
     8641 tagtcaggca tcggaatcag catggctgtc tgacccggct gcacacactc cttcagtgtg
     8701 ttgtagagat ggcggatcgc cgtggctttt ttagcggcct tttcgttcca agcccagagg
     8761 acaagcggtc cggggaaaat cgtcggcacc ttggccatta agaattgacg ggcggcttcc
     8821 tcatacgctt tttcttccga gacaatgacg ccattgatgt gggcttcacc cggattcggc
     8881 aagaagattc ccatggaagc cgctgcaggc ggaaggaaat gttcgggtcc agggagcaca
     8941 cgatattgac tcattacggc cttcactccg cgtatgggtt attgcttaaa aacgccaatt
     9001 ttcgaagaac ctacccgtga caatctaagc ttttttagag gatctctgca acctcgcaga
     9061 aggcccaacg tggggaaata atgggctact ggacaacaat ggcttaggcc ttttgaccag
     9121 tccccgcggg aaatcttgtc cgcgggttac atactcgata tcacggcgga tttcgcatta
     9181 taggagcggc actcgcgcga agtcaaacag acttccgtag gggaatatcg tggtgggtaa
     9241 gccgttaaga cttgaaaaca tgcgcaatta cggtggctta tattttcgcc gtcttacgga
     9301 agctgattgc aggctttgat ggatccgagc acgcacgcac gctcgaaatc atccttggcg
     9361 agccgccctt gtaggcgcgc ttggtagagg tgtgcccgcg tcacaagggc ggcgacatgg
     9421 gttggatcga tcaaaatgac ggtagacagg tcgtcgagcg cttgctctgg acggtgtagt
     9481 ttcagcagga cgtcgctgcg aagaagaaag gcttccttct ggtaggggcg ccagctgtcc
     9541 ggaaccggtg cgtcgagcag tttgtggagc gtcagaagcg actgctgcca ttgtttggct
     9601 ttggcctgtc ggcgagcctg agtggtatag aggccgatga ggcgctgcct cgccagatca
     9661 aaaaaagggt ctaacgccag ggcacgctca taggccagcg aagcctcatc ggtgcgcttc
     9721 agccaggttt ggacgtcacc ctttccgatc caggcccaag gattcttgac gtccagctcc
     9781 accgcttgat cgaaatcaga tctcgctttc tcgatatgcc ccgcgtactc gccagacttc
     9841 tggtgttctg aataggcgat cgagatcagc cgcaggtagg cttggcctct gacgatcaag
     9901 gg
//
LOCUS       DASTQY010000150        19106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1709531, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000150 DASTQY010000000
VERSION     DASTQY010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 19106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19106
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709531"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFR82_04835"
     CDS             complement(<1..196)
                     /locus_tag="VFR82_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500956.1"
                     /translation="MKVVLDIETIQSTREDWARLAGKCYSPIGEEPEYDLFAAGEAEA
                     QRQAENEQYAKSAFDGTYSSI"
     gene            complement(193..600)
                     /gene="mutT"
                     /locus_tag="VFR82_04840"
     CDS             complement(193..600)
                     /gene="mutT"
                     /locus_tag="VFR82_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HEU4500957.1"
                     /translation="MTKVVEVAAGLISHEGRYLIAKRRTGVHLAGLWEFPGGKREPGE
                     TLEECLQRELFEELSIRIDGPIPFRIIRHEYPEKTVELHFFRCTIGEGRATPLDCAEI
                     RWVHPSEMGQYEFPPADAPVIEALQRDSMGQNT"
     gene            complement(600..1319)
                     /locus_tag="VFR82_04845"
     CDS             complement(600..1319)
                     /locus_tag="VFR82_04845"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU4500958.1"
                     /translation="MALGTNSSLCRKKTPRRSLWPDRILKRRFQQRLLKWYASHGRDL
                     PWRKTSDPYHILVSEVMLQQTQVDRVVPKYLQFLDRYPSLQELAVAPVNQVKQIWYPL
                     GYNIRPERLHSIACETVERYGGQLPNNHEQLLSFKGIGRYTAGAIRSFAFNEDAPILD
                     TNVMRVLHRVFIGRGNPLRQKVRLWKLAEALIPPGRGYDFNQAMMDFGALRCTARMPS
                     CQGCTMKPICRTFPFTPRGKA"
     gene            1469..1957
                     /locus_tag="VFR82_04850"
     CDS             1469..1957
                     /locus_tag="VFR82_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500959.1"
                     /translation="MTFTASRPPWYSILVFSALLAGNDLLSVTGQATASPQQVPWECS
                     NYTDEAQTRCLNAFMERQQEQIGRLESQLQSQQDIVGQLKGQVERQTAATAQLQQQLA
                     QPPVTTAVPPPYVYPYAFAYPPVGIGLYLGRPWIGPGFYGYYGRPWGQRFYYYGHHGR
                     RR"
     gene            complement(1976..2695)
                     /locus_tag="VFR82_04855"
     CDS             complement(1976..2695)
                     /locus_tag="VFR82_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4500960.1"
                     /translation="MRRLTGKVAIVTGSSSGIGKAIALRFGEEGASVIVAARSAERCE
                     ETARAIRQKGGDSRVIQTDIRHEEQVDALFERTVQECGRVDIAVHNAGIFGGNRIAET
                     PTKAFDEVIRVNLRGTFLCCRAAFKQMKQQGGGTIINVSSVAGVQAWAGTGSYSASKH
                     GVMALTRALADEGRPFHIKVSAICPGGVAENLVDASESDILQSEKISPFDIAETAVYL
                     CSLGKFAVVHEVVVNRLGADW"
     gene            3154..3480
                     /locus_tag="VFR82_04860"
     CDS             3154..3480
                     /locus_tag="VFR82_04860"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4500961.1"
                     /translation="MVTRKHRRFVVTLPSTLVQRDQFRHKGSVRDLSAKGCRVESLIS
                     PFTGMQVSLLLQLPGESQPVKIENAAVRWCGSQGIGIEFLVVAEPEQARLGHFIQQLE
                     KTAPGN"
     gene            3525..3728
                     /locus_tag="VFR82_04865"
     CDS             3525..3728
                     /locus_tag="VFR82_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500962.1"
                     /translation="MELSSLSPGQLKELVRGLVDDRLRELIGDPDLGLELGEALRARL
                     KTSLEKGDRLSGDELAEKLGLRW"
     gene            complement(4006..4545)
                     /locus_tag="VFR82_04870"
     CDS             complement(4006..4545)
                     /locus_tag="VFR82_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU4500963.1"
                     /translation="MNYCGECGKSVTKKIPHGDNLPRFVCDSCQVVHYHNPKIVAGCI
                     AEWNGRILLCRRAIEPKSGLWTYPAGFMEIGEGTEEAARRETFEEARAKVRITRLHSV
                     LSLPHIGQVYLTFIGELLAEEFAAGEESLDARLFEQADIPWQDIAFPVVKDALRRYVE
                     DIRRGEFGVHVADLPGRLS"
     gene            4581..5756
                     /locus_tag="VFR82_04875"
     CDS             4581..5756
                     /locus_tag="VFR82_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEU4500964.1"
                     /translation="MDNTKYRFFAPCPRGLEGVLEDELRELGVSAIIRTQGGVGFHAA
                     WSNVYRINLKSHIASRVLWEIGHDTYRTEEDVYRAAYRVNWPEWFASSHTIKVKVTAQ
                     RCSLRSLDFLTLRIKDAVCDKFVALCGKRPSVERRQPDIRIDVFLASDGVTFYLDTSG
                     EPLFKRGYRQATAEAPLRENLAAGVLRLAGWTPDEVLMDPMCGSGTIPLEAALMARHS
                     APGFGRSFAFEHLRNYDTTQWADARKAAQTERRTEVQSVIYASDHNAAAIKTAARTFQ
                     AAGVGSDIQLQHSDVLSLNPPTAQGLLVLNPPYGMRLGDPTELRAFYPQLGDWLKQRF
                     VGWRAYVLTADFLFAKAIGLSPSRKIPLYNGPIECRLYEFKIVSGRPQRSRKVPSTN"
     gene            5990..7852
                     /locus_tag="VFR82_04880"
     CDS             5990..7852
                     /locus_tag="VFR82_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500965.1"
                     /translation="MRPMFDRLLQVPQVKLIEAHSTVETMSARYKRRLTLAALKGPWE
                     GFINLERQGLLLAELAEGRAVNLPALLDVLEASMDRTSAFNRPISIPAKATPQELMTF
                     MIESLEEASIHRDKALANLTEDERRFLFSHARTIVEQFTPQISSVSDMTIAQAKVDQR
                     FAELLEEQVDYANLMAAAQVLARLANEPWLRQLAGAFGQALPRSEVPGGITGDILLAQ
                     ATSYGMIVIGGAGPNTYELDHRFALIVDLGGDDLYRGLIAASADIEHGNAVIIDMSGN
                     DTYDSAALGLATGRLGVGLLIDQAGDDVYQLEIGSGGAGFGGLGILFDAKGNDLYMGT
                     RLTQGAAIGGLGLLFDTSGNDRYASHGFALGFGGPQGVGAVVDLQGDDEYQCGNKYPS
                     AYNEEDAPNAKPGDSMFQYDCFGLGTGSGRRILTKRPEWQAYDLAGGWGLLLDVEGND
                     RYRSANFSQGHGYFFGAGAFLDLSGNDEYVAARYGHGSSAHYGVGFFTDRQGADHYES
                     TGPFYNGGVAWDHGMSMMIDAGTDPDRYVFQSSNGLGKADYSGWGLFIDEGGNDSYQT
                     KDGYGLSSQRGIGGFFDLKGIDTYKLAPSTAEADLWPADGKVLLYPSGGLFVDR"
     gene            7961..10348
                     /gene="lon"
                     /locus_tag="VFR82_04885"
     CDS             7961..10348
                     /gene="lon"
                     /locus_tag="VFR82_04885"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEU4500966.1"
                     /translation="MNSTNLSVTLPVLPIKRTVLFPGILMPVTVGRERSVAAVNAALK
                     TEDKMILVVAQRDPAKENPGLDDLYTIGTKAMVKQVGRTEDGVIQALIQGIERVALLK
                     VEQAAPYLTVQARRLELPTDNDREVQALQRAIQDSITDLPRLVQAPGIEEATAAFRNE
                     ENPATLAYRVASLLNLTVKEEQTLLESSSTIELLRSLYAFLSKEIQILQLREKINSDT
                     QAKIGKNQREYVLREQLKAIQQELGEGDSEESEVAHLKKRIQEADLPEHVRKEVEREI
                     ARLSKVPPSSPDHQVLRTYLELVLELPWTKASEEHMNLPNVRQVLEEDHYGIKEVKER
                     IVEHLAVLKLNPTAKAPILCLVGPPGVGKTSLGQSIARAMGRRFERFSLGGVHDEAEL
                     RGHRRTYVGALPGRIIQAMRRAGVNNPVLMLDEVDKLGRDFRGDPAAALLEILDPAQN
                     HTFRDHYLDLPFDLSKVFFITTANTLDTISQPLLDRMEIIRVQGYSEREKAEIARRYL
                     WPRRLKEAGLEADHVQLPNGVLDRIISRYTREAGVRQLEQMLGRLTRKIALTVAELPE
                     SHARETVTVEEEKLPDWLGSERFMPEEARKHIPVGVATGLAWTPTGGDVLYIETTLLP
                     GSHEVSLTGQLGDVMQESARAARSYLWSHAESMGLDISRFKRNGLHIHVPSGAIPKDG
                     PSAGITMATALASSYVGKAVRSDTAMTGEISLSGLVLPVGGIKEKVLAAHRAGIKRII
                     LPKANEKDLKEVPQEVRDDLTFIFVERIEEVLPSAFDKDLGSPARPTEQHVSTAL"
     gene            10457..10879
                     /gene="msrB"
                     /locus_tag="VFR82_04890"
     CDS             10457..10879
                     /gene="msrB"
                     /locus_tag="VFR82_04890"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU4500967.1"
                     /translation="MPQKIDIGPIVKLAKSDDEWKKLLAPEAYQVLRHEATERPFTNN
                     MHDNKQAGIYHCAGCDLPAYSSEHKFDSGTGWPSFWQPIDPKVVETNTDFKLIFPRTE
                     VHCARCEGHQGHIFKDGPKPTGLRYCINGAALKFVPAS"
     gene            11012..11896
                     /locus_tag="VFR82_04895"
     CDS             11012..11896
                     /locus_tag="VFR82_04895"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4500968.1"
                     /translation="MAYVGFVGVGRMGANMARRLQDKGHRITAVHDRNLQAAEELRRE
                     LGCEAVDTPARVAELSEVVVTVVSDDAAMFDIYREGASSSLLAHAKGRLFINCATVTP
                     DVHIAMERIVERHEGASLEACMASSITQARQGTLYLMCAGKQPVFERARPLLEVMSAH
                     LRYIGRAGEAAKVKALVNMVMNCNTAALAEGLGLGSALGVDLTMLREVFSQTGAASRV
                     LETDGEDMQARAHDCYFSAAHATKDSAIALALAASVGLNLPLAEATHRQYERLVAAGR
                     GDLDKSAIAELTFKGRHD"
     gene            complement(11933..12712)
                     /locus_tag="VFR82_04900"
     CDS             complement(11933..12712)
                     /locus_tag="VFR82_04900"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEU4500969.1"
                     /translation="MLLKRGILSLIVLLAAQPVWAGIDETKLIDLTYALDEQTVFWPT
                     NRPFIWEKAAWGRTANGYWYASGDFSMSEHGGTHIDAPIHFAEGRLAVDEIPVQKLIA
                     PAIVIDTRSAVEENADYRLSMGDLEAWEARHGPIAQGAVVLMLTGWGNKWPDRMRYLG
                     SSTPSDPKTLHFPGFSQEAAEFLVKERHVDGIGIDTPSIDYGSSRDFIVHQIINGANL
                     YGLENVANLDKVPPKGAILVALPIKIRGGTGGPVRIIAILP"
     gene            12794..14971
                     /locus_tag="VFR82_04905"
     CDS             12794..14971
                     /locus_tag="VFR82_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU4500970.1"
                     /translation="MPTPNVLLLIPPLTQLNTPYPSTAYLTGFLRSQGVPCEQADLGI
                     EMVLRVFSREGLRQVFYQLEPRRRSLHPVAQEMIANQSAILDHIDAVVAYLQGKAPEL
                     TKSLSRRGFLPEGPRFRMQRSWSRNASIEDRAKHAATLFLEDLTDLIQVTITPHFALN
                     RYAEHIARSATSFDSMFAALQETPPLTDQFMLEALWAHVNRVNPSLVGLSVPFPGNLY
                     GAFRIAQSLKQLSPNIVIALGGGYVNTELRRLSEVRVFDFIDYVTLDDGERPLLCLLE
                     HLSGKRSVQALSRTYYRDNSRVVFADDMTLENIAMNDVGCPTYRGLKLDRYLSILDST
                     NPMHRLWSEGHWNKLTVAHGCYWKQCTFCDIGLDYISRYEITPTERLIRNIQQLADET
                     KRLTFHFVDEAAPPVALKTLAIALLQRNIGISWWGNIRFEEAFTPDLCRLLAASGCIA
                     VTAGLEAASDRLLAKMKKGITVDQTARVAAAFRQAGILVHAYLMYGFPSETVQETVDS
                     LERVRQLFAADLVQSAFWHRFTVTAHSPIGLDPQANGLRIVGPEFGGFAENDLLHEDS
                     TGPIPEWIGEGLRRSLLNFMESRGLAMEVTKWFDHHTPRPRVSPTWISRALSKRIADE
                     EFNPERRCVWIGEQACIESRTGHIKLSIQGRKRALATTLPSRQASWLGELLNEALPRR
                     DRYASYPSWRDAKLAFPGRATDFASFVKDPSWKQIRSAGLLLV"
     gene            complement(15005..16018)
                     /locus_tag="VFR82_04910"
     CDS             complement(15005..16018)
                     /locus_tag="VFR82_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU4500971.1"
                     /translation="MRAVGLYRYLSITDPESLVDLDIPKPEATGHDLLVRVEALSVNP
                     VDTKVRAAGKPERQTPLVLGWDAAGVVESVGQAVTLFSPGDTVYYAGDISRPGSNSQY
                     QLVDERIVGRSPRSLAVEEAAALPLVAITAYEALQDRLGIDFEGGSKGSTLLIIGGAG
                     GVGSMAIQLGKIAGLSVLATASRPESITWVAQLGADEVLDHRQPLKPQLERVGFREVN
                     FILNCADTDGYWDSMAELIAPQGKICTIVENKGPLQQQALKTKSITHVWEFMFTRPKY
                     HTVDMIEQHRLLNRVADWIDRGTLKGILRETLQPINAENLKKAHAKLESGTMIGKLTL
                     KGW"
     gene            complement(16096..16731)
                     /locus_tag="VFR82_04915"
     CDS             complement(16096..16731)
                     /locus_tag="VFR82_04915"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU4500972.1"
                     /translation="MALRLGDEAPNFTAETTEGTINFHEWLGNGWGILFSHPKDYTPV
                     CTTELGTVAKITSDFKKRGVKVIAVSVDPLESHKGWINDINETQHTTMNYPIIADPDK
                     KVSTLYDMIHPNALDNMTVRSVFVVGPDKKIKLTLTYPASCGRNFDELLRVIDSLQLT
                     SKYKVATPANWKEGEDCIITPAVSNDEAKTLFPKGFKTVKPYLRYTPQPNK"
     gene            complement(16822..>19106)
                     /locus_tag="VFR82_04920"
     CDS             complement(16822..>19106)
                     /locus_tag="VFR82_04920"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEU4500973.1"
                     /translation="SEGTLSLSGVKIATLFQYVQDQFHFDIPTGTIRAIGRYRFTAGP
                     PLGLQVSDTLLHFSDIGIVERGDSVPVIALHTLFLDGIHADLRQRKLEIETITLDKGA
                     HRIWRNADGSMNLPALLMPVQAQPVEEHATNEVAPRDASRAPAWTLSIKDASITHHNI
                     EFEDRALDFPARLKVSDLTVRTHDVVFPFKKPIPLTVKHRLNETGSVTVEGDIGVQPL
                     QADLAVSLKDIPVQPFEPYVEQFARIAVDTGALDLDGTLRFAAEHPKGPLMAFKGNLG
                     VKALAIADRDDGAPVASWKQVQLREIAIALDPTSVTIDEIGIHQPTVHLAIDRDGVPN
                     IKKLLSGAKAKNEPMGTERSSAQTKNTPSPSISVKTVKVLKGTATFRDESIAPPVQAG
                     LYDLTGTVKGLSSKQMARAEVNLSGKIDKVAPLKIAGTINPLTEDTFTDLTIRFDNVD
                     LTTAAPYTGKYVGYPIRKGKLFLDLVYKVSQKQLEAENKVAVDQLTFGEKTDSPDAMS
                     LPVQLAVALLKDRQGRIDINLPIRGDLKDPDFKYGKAVWATLGNLLTKMVASPFALMG
                     KLVPGGGDGEDLQHLAFEPGSTTIAPNEQKKIDALMKGLEERPGLRLEITGTADPARD
                     RQALALQMLEQQLRTRWRDKNGGAKNGDKPSPMDEERLIRELFDHWRSQQTPAPQKAD
                     PAQKPPTVEEMKRELLTTVNVEEDALRTLARTRAEQVHEQLVGEGKLPDERVFLTDVD
                     LAPSEDHEKVQSRLNITAGS"
BASE COUNT         4064 a         5403 c         5313 g         4326 t
ORIGIN      
        1 caatcgaact gtaggtgccg tcgaaggctg atttggcata ctgctcgttc tcggcctgcc
       61 gctgcgcctc ggcttccccg gcggcaaaca ggtcatattc cggttcttcg ccaatgggcg
      121 aatagcattt cccagccaag cgcgcccagt cttcgcgcgt agactggatc gtctcgatgt
      181 caaggacgac tttcatgtgt tctgacccat cgaatcccgc tgcaacgcct caataaccgg
      241 cgcgtcggcc ggcgggaatt catactgtcc catctccgag ggatggaccc aacggatttc
      301 cgcgcaatcc aacggagtcg cccggccctc accgatcgtg caccggaaga agtgcagttc
      361 gaccgttttt tcaggatatt cgtgccggat gattcgaaac ggaattggtc catcgatccg
      421 aatactgagt tcctcgaata attcgcgctg caaacactct tccagcgttt ctcctggttc
      481 tcgttttccg cctggaaatt cccataggcc ggccagatgg acgcctgtcc ggcgcttggc
      541 tatcaagtag cgcccttcgt gagaaattaa gcctgcagcg acttcgacca cctttgtcat
      601 caagccttgc cccgtggcgt gaacggaaag gttcggcaga tgggcttcat cgtgcatccc
      661 tggcaggacg gcatccgggc cgtgcaacgc agtgcaccga aatccatcat cgcctggtta
      721 aaatcatatc cccgtccagg cggaatcaac gcttcggcca gtttccacaa tctgaccttt
      781 tgcctcagcg gattgcctcg gccgatgaag acccgatgaa ggacccgcat gacgttcgta
      841 tcaagaatgg gagcatcttc gttgaacgcg aacgatcgga tggctccggc cgtataacgg
      901 ccgatccctt tgaaagataa tagctgctcg tggttattgg gcaattgacc gccgtaccgt
      961 tcgaccgttt cacaggcaat gctatgcaga cgttcgggcc gaatgttgta gcccagtgga
     1021 taccaaatct gtttgacttg gttgaccggc gccacggcaa gctcctgcaa actcggatag
     1081 cgatcgagga actgaaggta cttgggaacc acacggtcga cttgcgtttg ttggagcatc
     1141 acttcggaga cgaggatgtg gtagggatcg gatgtttttc gccatgggag atcgcggcca
     1201 tggctggcgt accacttcaa caggcgctgc tggaaccggc gttttaggat ccggtccggc
     1261 cacagcgagc gacggggcgt cttcttgcgg caaagagatg agttcgtgcc aagggccatg
     1321 gtaacgtgct gttgcggcgg cattgtagga gtctgcacgg acaaaatcaa acgcggcaaa
     1381 cctgcagaga tcgaaggcct tatgccacgc tggcgaacaa atcgatcgcg tggtaacgtg
     1441 tcgttgtaat tcccaaggag gtgatcccat gactttcacc gcctctcgtc ctccctggta
     1501 ttccatattg gttttcagtg cgctgctcgc cggcaacgac ctcttgagtg tgaccggtca
     1561 ggccaccgcc agtccccaac aagttccatg ggaatgctca aattacacgg atgaagcgca
     1621 gacccgctgc ctcaacgcgt tcatggagcg tcaacaggaa cagatcggca gactcgaaag
     1681 ccagctacag agtcaacaag acatcgtcgg acagttgaaa gggcaggtcg aacgtcaaac
     1741 tgcggcgaca gcacagttac agcagcaact tgcccaaccg ccggtcacga cagccgtccc
     1801 gccaccctac gtgtatccat atgccttcgc ctatccacca gtcggcattg gtctctacct
     1861 cggccgtcca tggatcgggc ccggtttcta tggttactat ggccggccgt ggggacagcg
     1921 cttctactat tatggccatc atggacgccg gcggtagact ggtatcgcag gtgcactacc
     1981 agtcggcacc cagccggttg acgaccacct cgtggactac cgcaaatttg ccgaggctgc
     2041 aaagatagac ggcggtttcg gcaatgtcga agggactgat cttctcgctt tgcaggatgt
     2101 cagactcgct cgcatcgacc agattttccg ccactcctcc cggacagatc gcagacactt
     2161 tgatgtggaa tggacggcct tcgtccgcca gtgctctcgt cagcgccatc accccgtgct
     2221 ttgaagcgct gtagctaccc gtgccggccc aggcttggac ccctgcaaca ctcgacacat
     2281 tgatgatcgt tccgccaccc tgctgcttca tctgtttaaa ggcggctctg caacagagaa
     2341 acgttccccg gagattcacg cggatcactt catcaaatgc cttcgtcggc gtctcggcaa
     2401 tcctgttgcc gccaaagatt cctgcgttgt ggacggcaat gtccactcgg ccacattcct
     2461 gcaccgtcct ctcaaagagg gcatccacct gttcttcatg cctgatatcc gtctgaatga
     2521 cccgggaatc gccacctttc tggcggattg ctcttgccgt ctcctcacaa cgttcggcgc
     2581 ttctcgcggc aacgatcacc gacgcccctt cctcgccgaa acgcagagcg atggctttgc
     2641 cgatgccgct gctgctgccc gtgacaatag ctacttttcc tgttaagcga cgcatgacga
     2701 tttagagacc atgtcaaaca tacggttttc tttgacaaag tacttagaca cccgtagcct
     2761 cattcttgca ttagtttagc gggaatgtgc tgcgttcttt actttttgca tcccttccgg
     2821 gagagcgctc gtcatggacc agtgcgggca cgaacgatcg tcgcggaact cccagcgcga
     2881 ttgttcggag attctggaac tacggccatc gtatacaacc ttggaacggc tgttgcaaga
     2941 ttctgacgaa tcagccttga tcgtcgatgg gatgttggcc cttgggttag acatgccgcg
     3001 cgtgacgagg gccattggtg tccgaatggg ggcgctgggg cccccactag gaattcgggc
     3061 tagagttggc atgggagtat ttgatcgcaa gcggcctgct caattaattc gccaaccggc
     3121 cgccgctgcc gcttaagtga aaggacccaa cgcgtggtca cccgcaaaca tcgccggttc
     3181 gttgtcacct tgccgagtac attggttcaa cgggaccagt ttcgacacaa aggctctgtc
     3241 cgtgaccttt cggctaaagg ctgccgcgtg gaaagcttga ttagcccttt cacgggcatg
     3301 caggtctcgt tgcttcttca gcttcctggt gaatcccagc ctgtcaagat cgagaatgcc
     3361 gcggtccgat ggtgcggctc gcagggcatc ggcatcgaat tcctggtcgt ggcggaaccc
     3421 gagcaggctc gcctgggcca tttcattcag caattggaaa agacggcgcc aggcaactag
     3481 tctccgattg catttcttgg ctctgctctc tattctttcc ctccatggaa ctttcctcgc
     3541 tctcgcccgg ccaactcaag gaattggtgc gaggtcttgt cgacgaccgt ttacgggagt
     3601 tgattggtga tccggatttg ggactcgaac tcggggaagc gctgcgcgcc agactcaaaa
     3661 cttctctcga gaaaggcgac aggctatccg gtgatgaact ggctgagaag ctaggactac
     3721 gctggtaaat cgatgccctg gtttcggctc accttccaag ctggcgtcgt tcaggacctg
     3781 gctgtcgcag ggaacccgat ggcgcagcgg cttctagaca agacaaagtg gattgcatcc
     3841 aatacagaga atcttcggca tgagccgatc ggcgatctgc cgggattgca taagtacgct
     3901 gtggctgact ggcgaatctt ttatgagatc cttcggacgg agtcgctcgt gaacgtacac
     3961 gcaattgtcc acaagagtac cttgcagcga tgactccgcc tgccatcaag aaagacggcc
     4021 cggcaagtct gcgacatgca cgccgaactc ccctcgcctg atatcctcga catagcgacg
     4081 caaggcatcc ttgaccacag ggaacgcaat atcctgccag ggaatatcgg cctgctcaaa
     4141 caaccgtgca tcaagacttt cttctccggc tgcgaactcc tcggccaaca actctccaat
     4201 aaacgtcagg taaacctggc caatatgggg aaggctcagc accgaatgca atctcgtgat
     4261 tcgcactttt gccctggctt cttcaaacgt ctcgcgccta gccgcctcct cggttccttc
     4321 accgatctcc atgaatccgg ccggataggt ccacagtccg gatttgggtt cgatggcacg
     4381 tcggcagagc agaatgcgac cattccactc tgctatacat ccggccacaa tctttgggtt
     4441 atggtagtga acgacctggc acgagtcaca gacgaatcgt ggcaaattgt ctccgtgagg
     4501 gatctttttc gtgactgatt tgccgcattc accgcagtaa ttcatgggtt gttttccacg
     4561 ttgctctgaa cattctccga atggataaca caaaatacag attctttgca ccctgccccc
     4621 gtgggctgga gggcgttctt gaagacgaac tccgcgagct tggagtttcc gccataatcc
     4681 ggacgcaggg aggcgttggc tttcatgctg cgtggtccaa cgtgtatcgg atcaacctca
     4741 agagccacat tgccagcaga gtcctgtggg aaataggtca tgatacctat cgcacggaag
     4801 aggacgtcta tcgcgctgcc tacagggtga actggccgga gtggttcgca tcttcgcaca
     4861 ccatcaaggt gaaagtgact gcgcaacgct gttctcttcg cagtttggat tttctgaccc
     4921 ttcgtatcaa ggacgccgtc tgcgataaat tcgttgcctt gtgcggaaag cggccgagtg
     4981 tcgagcggcg acagcctgat atcaggatcg acgtcttcct cgcttccgac ggcgtgacat
     5041 tttatctcga tacgtccggg gagccgcttt tcaaacgtgg atatcgtcag gcaacggctg
     5101 aggcgccttt gagagaaaat ctcgctgctg gagtgcttcg gctggccgga tggacccctg
     5161 atgaagtgct catggatccg atgtgcggca gtggaaccat tccgctcgaa gcggcgctca
     5221 tggcccggca cagtgcgcca gggttcgggc gctcgttcgc ctttgaacac ctgcgcaact
     5281 acgacacaac acaatgggct gacgcccgta aggctgccca aaccgagcgt cggacggaag
     5341 tacaatcagt catttacgcc tcggatcata atgccgctgc gatcaagacg gccgcgcgca
     5401 cgtttcaagc ggcgggcgtg ggttcggaca ttcaactcca acactcggat gtgctgtcgc
     5461 ttaatcctcc gacggcccaa ggattgctcg ttcttaatcc cccttatgga atgcgactcg
     5521 gcgatccgac tgagctccga gccttctatc cgcagttggg ggattggctg aaacagcggt
     5581 tcgttggatg gcgtgcctat gttttgacgg ctgatttcct ttttgcgaag gcgatcggcc
     5641 tttctccgtc tcggaagatt ccgctttaca atggccccat cgaatgtcgt ctttatgagt
     5701 ttaaaattgt cagtgggagg ccccaacgtt cacgcaaagt tccttcaacc aattaagcaa
     5761 gctcaccctt ctcactgtac atacgagaag gtaccgccct tggaatttct tgccgtctga
     5821 aacctcgcgt ctcgtgattc gactgctcct gtccgcgagc ctcctggttc tacttgccct
     5881 ccccggatgc agccttgttc aagctttttt tgcctcgctg ggtgtgccgg agggggctgt
     5941 caccaatgct ccaatgcgag actcgtcggt tatgcgccta gagccgatcg tgcgtccgat
     6001 gttcgatcgg ctgctgcaag tcccccaggt gaaactgatc gaagctcatt ccacggtaga
     6061 aacaatgagt gcacgatata aacgtcgtct tacattggct gccctgaaag gaccttggga
     6121 gggtttcatt aatctcgagc gacagggact gctgttggcg gagttggccg aggggcgtgc
     6181 cgtcaatttg cctgctctcc ttgatgtatt ggaagcaagc atggatcgga cctctgcgtt
     6241 caatagacct atttcaatcc ccgcgaaggc taccccccag gagctcatga cgttcatgat
     6301 cgaaagcctc gaagaagcgt cgatccatcg tgacaaggcg ctcgcaaatc tgaccgaaga
     6361 cgaacgtcgt tttctcttca gccatgcgcg gaccatcgtc gagcaattca cgccgcagat
     6421 ctcaagcgtt tccgacatga ccattgcaca agcaaaagtg gatcagcgtt ttgctgagct
     6481 gctggaagaa caagtggact atgccaatct tatggcggca gcgcaggtct tggcccgctt
     6541 agccaacgag ccctggctcc gccagctcgc cggtgcgttc ggtcaggcgt tgcccaggtc
     6601 cgaggttcct ggaggcatca ccggagatat cctgctcgcc caggcgactt cctatggaat
     6661 gatcgttatt ggcggagccg gaccgaatac atacgaattg gatcatcgat ttgcgttgat
     6721 cgtcgatctc ggaggggatg atttgtaccg aggcctgatc gcggcgtccg ccgatatcga
     6781 acacggcaat gccgtgataa tcgacatgag cggcaacgac acctatgaca gtgcggcgct
     6841 cgggcttgcc actggacgcc tgggcgtcgg cctgctcatt gaccaggcag gcgacgatgt
     6901 ttaccagctc gagatcggtt caggcggcgc cgggttcggc ggtttaggga tcctgttcga
     6961 tgccaagggc aatgacctgt acatggggac ccgcctgacg caaggtgcag cgatcggggg
     7021 gttgggcttg ctgttcgaca cgtcgggcaa cgatcgttat gccagccatg gattcgccct
     7081 gggattcggc ggtcctcaag gagtcggtgc ggtggtcgac ttgcaaggtg acgatgagta
     7141 tcaatgtggc aacaagtacc ccagcgctta taatgaagaa gacgctccca atgccaagcc
     7201 cggagattcg atgtttcaat atgattgttt tggtttgggg acaggctccg gaaggcgaat
     7261 actcacgaag cgaccggaat ggcaggccta tgacctggcg ggaggatggg gacttttgct
     7321 cgacgttgaa ggcaatgacc gatacaggag cgccaatttc tcccagggcc atggatattt
     7381 tttcggcgcc ggcgcattcc tcgatctgag cgggaacgat gagtacgtcg cggctcgcta
     7441 cggtcatggt tcttccgctc actacggtgt cggctttttc accgaccgcc agggcgccga
     7501 tcactatgag tcaacgggac ccttctacaa cggcggagtt gcatgggacc atgggatgag
     7561 tatgatgatc gatgctggaa ccgaccccga tcgctatgtc ttccagtcct ccaacggatt
     7621 gggaaaagca gattactccg gctggggact gttcatagac gaaggcggaa acgacagcta
     7681 tcagacaaag gacggatacg gtctctcctc tcagcgcggc atcggcggct ttttcgatct
     7741 gaaggggatc gatacatata aactggctcc gtcaacggct gaggcagatc tctggccggc
     7801 cgatggaaag gtccttctct atccgagcgg tggattgttc gtcgatcgtt agagcgcgct
     7861 aatctcgttg cccgccactc ttccttgact tctcctggac acaacctatc tgtccgttac
     7921 aactcatgtc agctcacttc gcgagcggag gttatcacgc atgaacagca caaatctttc
     7981 ggtgacctta cccgtcctcc caatcaagcg cacggtattg tttccgggaa tactgatgcc
     8041 ggtaacagtg gggcgtgagc gctccgtcgc ggccgtgaat gcagccctga aaacggaaga
     8101 caaaatgatt ctcgtcgtcg cccaacggga tccggccaaa gagaatccag gactggacga
     8161 cctctacacg atcggcacga aagccatggt gaagcaggtg gggcgcaccg aggatggcgt
     8221 catccaggcg ctcattcaag gtatcgagcg tgtcgcgttg ctcaaagtcg agcaagctgc
     8281 cccctatctc acggttcagg cgcgccggct cgaactgccg acagacaatg atcgagaggt
     8341 gcaggcactt caacgggcta ttcaagactc catcaccgat cttcctcggc tggttcaggc
     8401 gcccggcatc gaagaggcca ccgcggcgtt tcggaacgaa gaaaatccag ccacgttggc
     8461 ctatcgcgtc gcgtcgctgt tgaatcttac tgtcaaggag gagcagacat tactcgaaag
     8521 ttcttcgacg atcgaactgc tgcgatcgct ctatgcgttt ctgtccaaag aaatacagat
     8581 tcttcaactc cgggagaaga tcaatagtga tactcaggca aaaatcggga agaatcagcg
     8641 tgaatatgtc ttgcgcgagc aattgaaggc gatccagcag gagctcggcg aaggagactc
     8701 cgaagagagt gaagtcgccc atctgaagaa acggatccaa gaggcggact tacccgagca
     8761 cgtgcgaaag gaagtcgagc gcgagattgc caggctctcg aaggttccgc cttcttcacc
     8821 cgatcaccag gtattgagga cctatctgga acttgtactc gaattgcctt ggaccaaggc
     8881 ctctgaagag cacatgaatc tccccaatgt gcgccaggtg ctggaagagg accactatgg
     8941 gatcaaagag gtgaaggaac ggattgtcga gcacctggct gtgttgaaat tgaatcccac
     9001 cgccaaagct cccattctct gcctggtcgg gccgcccggt gtcggtaaga cgagtctcgg
     9061 tcaatcgatt gccagggcga tgggacggcg cttcgagcga ttcagtctcg gcggcgtaca
     9121 cgatgaggct gagctccgcg ggcatcgccg gacctatgtc ggcgccttgc ccggacggat
     9181 catacaggcg atgcgtcgag ccggggtcaa caatcccgtc ctcatgttgg acgaagtgga
     9241 caagttgggc cgcgacttcc gcggcgatcc cgcggcggcg ctcctggaaa tcctggatcc
     9301 ggcgcaaaac catacgttcc gggatcacta tctcgacttg ccattcgatc tctcgaaagt
     9361 gtttttcatt acgactgcga acaccttgga tacgatcagc cagccgctct tggaccgaat
     9421 ggaaatcata cgggttcaag ggtacagcga aagagaaaaa gccgagattg cgcggcgtta
     9481 tttgtggccg cgacgactca aggaggcggg tctggaggca gatcatgtcc aacttcccaa
     9541 cggagtattg gatcgcatca ttagtcgata cacgagggag gctggtgtcc ggcaactgga
     9601 gcaaatgctg ggtcgtttga cccgcaagat cgcgctcacg gtagccgagc tccccgagag
     9661 ccacgcccgc gagacggtca ctgtggagga ggagaaactt cccgactggc tcggatcgga
     9721 acggttcatg ccggaagaag cgagaaaaca tattcccgtg ggcgtcgcga ccggtctcgc
     9781 ttggacaccg accggtggcg atgtattgta tatcgaaacc actctcctgc ccggaagtca
     9841 cgaggtcagt cttaccggtc agttgggtga tgtgatgcaa gagtcggcga gggccgcacg
     9901 aagctacctg tggtcgcatg ccgaaagcat gggactcgat atctcacgat tcaaaaggaa
     9961 cgggctgcat atccacgtcc cttcaggagc cattccgaag gacgggcctt cagcgggcat
    10021 tacgatggcg accgcactgg catcgtccta tgtggggaag gcggtgcgaa gcgataccgc
    10081 catgactggt gaaatcagcc tcagcggctt agtcctaccg gttggcggga tcaaggagaa
    10141 agtgctggcc gctcatcggg caggcattaa acggatcatt cttcccaagg cgaatgaaaa
    10201 ggatctcaaa gaagtgcctc aagaagtgcg ggacgaccta acgtttattt ttgtcgagcg
    10261 aatcgaggag gtactcccct cggcgttcga taaagatctg ggaagcccgg ctcggccgac
    10321 cgagcaacat gtatcgactg ctttgtaatt cggggaagga gcaaggcggc cggaccggcc
    10381 gccttgctcc tcgaccaata cgtatgccag gatgtctcgc atctggagta gtcctgagtt
    10441 acccgggagt ttgttcatgc cgcaaaagat cgacataggc cctatcgtca aactcgccaa
    10501 gtccgacgac gaatggaaga agctcttggc gccggaggcg taccaagttt tgagacatga
    10561 agcgaccgag cgaccgttta cgaataacat gcatgacaat aaacaggccg gtatctatca
    10621 ttgtgccggc tgtgatcttc ctgcctattc atcggagcat aaattcgaca gcggcaccgg
    10681 ctggcccagc ttttggcagc caatcgatcc gaaggttgtc gagacgaata cagatttcaa
    10741 gctcattttc ccgcgtacag aggttcactg cgctcgatgc gaagggcatc agggacatat
    10801 cttcaaagac ggcccgaaac ccacaggcct ccggtattgc atcaacggtg cagcactcaa
    10861 attcgtacct gcctcttagc atgatggtcc tggccgctta agcaccactc ctgttcgacg
    10921 cccggtcttg agggacgcag acactccctt gatacgcgga taatcgggca catcacctaa
    10981 aatcgatatc attcagggaa ggaacgaaac tatggcatat gtcggctttg ttggtgtcgg
    11041 acgcatgggc gccaatatgg ctcgccgcct gcaggacaaa ggtcatcgca tcacggccgt
    11101 tcacgaccgc aatttgcagg cggccgagga gctcaggcgc gaattaggat gtgaggcagt
    11161 ggacactccg gcacgcgtcg ctgaattgtc agaggtcgtt gtcaccgtag tgtccgatga
    11221 cgcggccatg ttcgacatct accgtgaggg ggcgtccagc agtctcttag cgcatgccaa
    11281 aggccgtctt tttatcaatt gcgcgacggt gaccccggat gttcacattg ctatggaacg
    11341 gattgtcgaa cgccacgaag gcgccagcct ggaagcctgt atggccagca gtattactca
    11401 agcccggcaa ggcaccctct atctcatgtg cgcaggcaaa cagcctgtct tcgagcgcgc
    11461 cagaccgttg cttgaagtca tgagtgcaca ccttcgttat attggccggg cgggagaggc
    11521 cgccaaggtg aaggccctcg tcaacatggt catgaattgc aacacggcgg cgctggccga
    11581 agggctcggc ctaggtagcg ctcttggagt cgacctgacg atgctgcgcg aggtgtttag
    11641 ccaaaccggc gccgcctcac gcgtattgga aactgatggc gaagatatgc aggctcgagc
    11701 ccacgactgc tatttttctg cagcccatgc caccaaggat tccgcaattg cgctcgcact
    11761 cgcagcgtcc gtgggattaa acttgccttt agctgaagca acccaccggc aatatgaacg
    11821 gctggtagcg gccgggcgag gcgatttgga caaatccgcc attgccgagc tcacctttaa
    11881 agggcgtcat gactgatgac cttgggacca tcccttcata gagacaattc tgtcagggta
    11941 ggatggctat aatcctgacg ggccctcctg tcccgcctcg gattttgata ggcaatgcca
    12001 cgaggatcgc tcccttgggc gggaccttgt cgagattggc gacgttttca aggccgtaaa
    12061 gatttgcacc gtttatgatc tggtgcacga tgaaatctcg ggaggagcca tagtcaatgc
    12121 tcggcgtatc gatgccgatt ccatcaacat gccgttcctt cacaagaaat tccgcagcct
    12181 cttgcgagaa gcccggaaaa tgcaacgttt ttggatctga tggtgtagaa cttccgagat
    12241 agcgcatccg atccggccat ttgttccccc acccggtaag catcaatacc actgccccct
    12301 gagcaatcgg tccatggcga gcttcccagg cttcgagatc acccattgag agtcgatagt
    12361 cggcattttc ttcaaccgct gaacgggtat cgatgacgat ggccggcgcg atgagcttct
    12421 gcacggggat ctcatccacg gccagccggc cttctgcaaa atgaatgggc gcatcgatat
    12481 gggtgccgcc atgttcagac atggagaaat cgcccgaggc ataccaataa ccattggcag
    12541 tcctacccca ggcagctttc tcccagataa acggcctgtt cgtaggccag aacaccgtct
    12601 gctcatcgag ggcgtaggta agatcgatca gttttgtttc atcaatcccc gcccaaaccg
    12661 gttgagcggc aagcaggacg atgagactca agattccgcg tttcagtaac atagttggct
    12721 cctcatggac tccagctgcc gtttactgcc atccttcctg gctttcattt cctgcactat
    12781 gttatctcaa gcgatgccta cgccgaatgt cttactgctc attcctccgc taacgcaact
    12841 gaataccccc tacccttcca cggcctattt gaccggcttc ctgcgctcgc agggtgtccc
    12901 ctgcgaacag gccgatctcg gcatcgagat ggttctccga gtattcagcc gggaaggtct
    12961 gaggcaggtg ttctatcagc ttgagcccag aaggcggtcg cttcatcctg tggctcagga
    13021 gatgatcgcc aatcaatccg ccatcctgga tcacatcgat gccgttgtgg cctatcttca
    13081 ggggaaagca ccggaactca cgaaatcact ttcacgacgg gggttccttc ctgaagggcc
    13141 gcgttttagg atgcagcgca gctggtctcg aaacgcatcg attgaagacc gggcgaaaca
    13201 cgcagcgacc ctatttctgg aggacctcac ggatctgatt caggtaacca tcacgccgca
    13261 ttttgcgctc aaccgctatg cggaacacat cgcacgatcg gctacttcat tcgacagcat
    13321 gtttgctgcg cttcaagaaa cgccacccct gaccgatcaa ttcatgctcg aagcactgtg
    13381 ggcgcatgtg aaccgcgtga acccctcgct cgtcggcctc tcggtgccct ttcctgggaa
    13441 cctctacggt gcttttcgca tcgcccaatc gctcaaacag ctaagtccta acatcgtgat
    13501 cgcccttggc ggaggctacg tgaataccga gctccggcgt ctgtctgaag tgcgagtgtt
    13561 cgattttatc gactacgtca cgttggatga cggcgaacgg cccttactct gcctgctgga
    13621 gcatctgtcc ggtaagcggt ccgtgcaagc gctcagtcgg acgtattacc gtgacaacag
    13681 tcgagtggtg tttgccgatg acatgacgct tgagaacatt gcgatgaacg acgtcggctg
    13741 tccgacgtat cgcggcctga agctagatcg gtacctgtcg attcttgaca gcactaatcc
    13801 catgcatcgg ctgtggtcgg aaggccattg gaacaagttg acagtcgcgc acgggtgtta
    13861 ttggaagcag tgcacgtttt gcgacatcgg acttgactac atcagtcgct acgaaatcac
    13921 gccgacagag cgtctcatcc gcaacatcca gcagttggcc gacgagacga agcgtctcac
    13981 ctttcatttt gtggacgaag ctgcgccccc ggtggcttta aaaacattgg cgatcgccct
    14041 cctgcaacgc aacattggta tttcttggtg gggcaacatt cgttttgaag aggcgttcac
    14101 gccggatctc tgccgcctct tggcggcgtc cggttgcatc gccgtcactg ccggtctgga
    14161 ggcggcctca gatcgattgc tggccaagat gaagaaaggc atcacggtcg accagacggc
    14221 ccgcgtggcc gccgccttca ggcaggccgg aattctcgtg catgcctacc tcatgtatgg
    14281 ctttccgtct gagaccgtgc aggaaaccgt cgattcacta gaacgcgtcc gccagttgtt
    14341 cgcggctgac ttggttcagt ccgcattttg gcatcgattc accgtcactg cgcacagtcc
    14401 gatcgggctc gatccgcaag ccaacggcct tcgcattgtg gggcctgagt ttggcgggtt
    14461 cgccgaaaac gatctgctcc atgaggattc taccggtccg atccccgaat ggatcggtga
    14521 aggactgcgt cgatcgttgc tcaactttat ggaatcacga gggttggcga tggaggtcac
    14581 gaaatggttc gatcaccaca ctccaaggcc gagagtgtca ccgacttgga tcagccgtgc
    14641 cttgagcaag cgcattgcgg acgaggaatt taatccggag cgccgctgtg tctggatcgg
    14701 cgagcaggcg tgcatcgaat ccagaaccgg tcatatcaag ctctcgattc aaggccgaaa
    14761 gagggcgctg gccactactc ttccgtcgag gcaagcgtcg tggttgggtg agttgctgaa
    14821 cgaggcgctt ccgcggcgag atcgctatgc atcttatccg tcatggcgtg atgccaaact
    14881 agccttcccc ggcagagcga ctgattttgc ctcgtttgtg aaagatcctt cctggaagca
    14941 gatccgcagt gcgggacttc tgctggttta acgatgcctc ataaccccga tccctcgctg
    15001 gtcgctacca gcccttcagg gtaagttttc cgatcatcgt gcctgattcc agcttggcat
    15061 gtgccttttt caggttctcc gcgttgatgg gttgcagggt ctcgcggaga attcccttca
    15121 gagtaccgcg atcgatccaa tcagccactc gattgagcaa gcggtgttgc tcaatcatgt
    15181 cgaccgtgtg gtacttcggt cgggtaaaca tgaactccca cacatgggtg atgctcttcg
    15241 ttttcaaggc ctgttgttgc aagggcccct tgttctcgac aatcgtacag atcttcccct
    15301 gcggagcgat cagctcagcc atactgtccc agtagccgtc cgtgtcggca cagttcaaga
    15361 tgaagttgac ctctcgaaag ccgacccttt cgagctgcgg cttcagcggc tgccgatgat
    15421 cgaggacctc gtctgcaccg agctgcgcga cccaggtgat cgattctgga cgggatgctg
    15481 tggccaagac tgaaagtcca gcaatctttc cgagttgaat cgccatggaa ccgacgccac
    15541 cggcgccgcc gataatgagg agggtgctgc ccttcgaacc accctcgaag tctattccaa
    15601 gacggtcttg cagggcttca tacgcggtaa tggcgactaa cggaagggct gctgcttctt
    15661 cgactgcgag cgaacgcggg ctccttccga caatgcgctc atcaaccagc tgatattggc
    15721 tgttgcttcc gggtctggaa atgtcgccgg cataatagac ggtgtcccca gggctgaaga
    15781 gagtcaccgc ctgcccgaca gattcaacca ctcccgccgc atcccatccg agcacaagcg
    15841 gggtctgcct ctctggtttg cctgcagcgc gcactttcgt atcgacaggg tttaccgaca
    15901 gggcctcgac gcggacgagc aggtcatgtc cggtggcttc cggcttggga atgtcgaggt
    15961 cgaccaggct ctccgggtct gtaattgaaa gatatcgata cagccctact gcgcgcatgc
    16021 agctctccat atgagaaaac agaaagggcg ggaagagatg gggatccctt cccgccctgc
    16081 attaaacctg ttcggctatt tgttcggctg tggtgtatag cggagataag gcttcaccgt
    16141 cttgaaaccc ttcggaaaga gcgtcttcgc ctcatcgttg ctgacagccg gggtaataat
    16201 acaatcctcg ccttctttcc agttggccgg ggtcgccact ttatacttcg acgtcagttg
    16261 aagcgagtct atgacacgca ataattcatc gaagtttcga ccacatgaag caggatacgt
    16321 caaggtcaac ttgattttct tgtccggccc aacgacaaag acagagcgca cagtcatgtt
    16381 atcgagtgcg ttgggatgga tcatgtcata cagcgtcgaa accttcttat ccggatctgc
    16441 gatgatggga taattcatgg tggtgtgctg cgtctcgttg atgtcgttga tccagccttt
    16501 atgcgattcc agcggatcca cactgactgc gatcactttg accccccgct tcttgaaatc
    16561 agacgtgatc ttcgccacgg tgccgagctc ggtggtgcag acgggggtat aatcttttgg
    16621 atgcgaaaaa agaattcccc acccgttgcc gagccactca tgaaagttga tggtcccttc
    16681 ggttgtctca gccgtgaaat tcggagcttc atctcctaat cgtagagcca tgaccgacct
    16741 ccttagttat ccaagtcctt aagagctaat gaacaagcta cgatactgtc cctttcgaag
    16801 gaagtgcaag aggtaactca gctacgagcc ggccgtaata ttcaagcggc tttgcacctt
    16861 ctcatggtcc tccgatggtg cgagatcgac gtcagtcaga aacacgcgtt catcgggcag
    16921 ttttccttca ccgacaagct gttcatgcac ttgctcggct cgtgtgcggg ctagagttcg
    16981 aagcgcatcc tcctccacgt tcacggtagt caacagctca cgcttcatct cttcaaccgt
    17041 cggcggtttc tgtgcaggat ccgctttttg tggagccgga gtctgttggc tgcgccagtg
    17101 atcgaagagt tccctgatca ggcgttcttc atccatggga gacggcttat cgccattctt
    17161 ggcacccccg tttttatcac gccatctagt tcggagctgc tgctcgagca tctgaagcgc
    17221 aagtgcctgg cgatcacgcg cgggatcggc agttccagta atttccaagc gcaaccctgg
    17281 ccgttcctcc aaccctttca tgagagcgtc aatcttcttc tgctcgttcg gagcgattgt
    17341 cgtcgaccct ggctcgaacg cgagatgctg caggtcctct ccatctccgc cgccaggaac
    17401 caacttgccc ataagagcaa agggtgacgc gaccatcttg gtcaaaagat tgcccaaggt
    17461 ggcccacacc gccttcccgt atttaaaatc aggatccttg agatctcctc gtatgggaag
    17521 attaatgtcg atcctgccct gacggtcctt taacagggca acggccagct ggacgggcaa
    17581 ggacatggca tcggggctgt cggtcttctc gccaaacgtg agttggtcaa ccgccacctt
    17641 gttttcggct tccagttgct tctgcgagac cttgtacacc aggtccaaga acagttttcc
    17701 tttcctgatc ggatagccga catacttgcc ggtgtagggg gcggcagtgg tcagatcgac
    17761 attgtcgaat ctaatggtca aatcggtgaa ggtgtcctca gtcaacgggt taatggttcc
    17821 cgcgatcttc aggggagcca ctttgtcgat tttgccggaa agattcacct ccgccctggc
    17881 catttgtttc gacgagagcc ctttcaccgt gcctgtcaga tcgtagagtc cagcctgcac
    17941 cggcggtgca attgattcat cccggaacgt ggccgtcccc ttgagcacct tgaccgtctt
    18001 gaccgagatg gaaggcgacg gagtgttttt cgtctgggct gacgatcgct cagtacccat
    18061 cggctcgttc tttgctttcg caccggatag cagtttctta atgttcggta cgccgtcccg
    18121 atcgatcgcg aggtgcacgg tcggttggtg gatcccgatc tcgtcgatgg tgacgctcgt
    18181 cgggtcgaga gcgatggcga tctcccgcaa ttgtacttgc ttccaagagg ccacaggagc
    18241 gccgtcgtcc cggtcggcga tggccagcgc cttcacgccg agattcccct tgaaagccat
    18301 gagtggcccc ttcgggtgtt cagccgcaaa tcgaagcgtt ccgtccaaat caagggcacc
    18361 agtgtccacg gcaatgcgcg caaactgctc aacatacggt tcgaaaggct gaactggaat
    18421 gtctttcagc gagacggcca aatcggcctg caacggttgc acaccgatat ccccttcgac
    18481 tgtcacgctc cccgtctcgt tgaggcgatg cttcaccgtt agtggtatgg gcttcttgaa
    18541 cggaaacacc acgtcgtgcg tccgaacggt cagatcactg actttcagtc tcgcgggaaa
    18601 atcgagtgcg cgatcttcaa actcaatatt gtggtgagtg atgctcgcgt cctttattga
    18661 gagagtccat gccggcgccc tgctcgcgtc acgtggggcg acttcgttag tggcgtgttc
    18721 ttcaaccggc tgcgcttgaa ctggcatcaa gagggccgga agattcatcg atccgtctgc
    18781 attgcgccaa atccggtggg ctcctttatc cagagtaatg gtttcgatct cgagcttccg
    18841 ctgccgaaga tcagcatgga tgccatctag gaacaatgtg tgtaacgcga tgacgggaac
    18901 ggaatcgcct ctttcgacga tgccgatgtc gctgaagtgg agcaaggtat ccgacacttg
    18961 gaggccgagc ggtgggcctg cagtgaagcg gtagcggcca atcgctcgga tggtcccggt
    19021 gggaatgtca aagtgaaatt gatcctggac atactgaaag agcgtcgcga tcttgacgcc
    19081 gctgagagaa agcgtacctt cagacg
//
LOCUS       DASTQY010000151         4262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_58885, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000151 DASTQY010000000
VERSION     DASTQY010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58885"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFR82_04925"
     CDS             <1..543
                     /locus_tag="VFR82_04925"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEU4500974.1"
                     /translation="FCLGLSSGFLEPLESTSIYLVQRGLQYLLSSFPQAVPNPALQEN
                     VNRRNRAHWDHIRDFIVLHYKMNKREGEPFWDECRNMPIPESLAESIELFRATGRFTK
                     DATEFFRPSSWLSMYAGFGLLPRYYSPYVDDVPDAQLVKEFESMAAGIKNAVTGAPTH
                     EEFIRANCATIVPMQSAANA"
     gene            587..1258
                     /locus_tag="VFR82_04930"
     CDS             587..1258
                     /locus_tag="VFR82_04930"
                     /inference="COORDINATES: protein motif:HMM:NF039455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HEU4500975.1"
                     /translation="MSTSHVGAERLPVVCIDDFSVNPALLVAIACEAVFIDVGSRYPG
                     VRAPAPQAYTNALLSATAATIEQAFGAPPEADLDLCAFSMVTTAPEKLRPIQRIPHFD
                     GPDRSRIAFIHYLCAPYQGGTSFYRHRATGLAEITPDNVERYRSKVVDELKADSPVQA
                     YVIDHTHFFERLHGVEAAFNRLIIYKGNALHSGDISARTILSEDPRQGRLTINGFGFL
                     RPSSH"
     gene            1279..3426
                     /locus_tag="VFR82_04935"
     CDS             1279..3426
                     /locus_tag="VFR82_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEU4500976.1"
                     /translation="MERTKVFHRARWIWLLCAAGMFALAGSKVTTQDRNHDRKEDRNH
                     DYGHDRKDDRNRDHDRKDDRNLGHDRDHDSDRKGRSSFVTREGSDLYLQGRLFRFAGS
                     NNYYPIYKSPLMVTALLDKAAVSDFAVMRVWSTVVIGNQDGSNSVDGIKEGVYFHYWD
                     GAAPAFNDGPDGLQRLDFVVAEAAKRKIKLVLPLVNNWNEFGGMNQYVRWRGGQYHDE
                     FYTDATIKGWYKEWISHLLNRKNSITGLRYKDDPTIMMWELANEPRCIGSGGANGYPR
                     SPNCTTDTLVSWAGEMSKYIKSIDKNHLVSVGDEGFLCLPNGTDWTDNCSEGVDSYAF
                     AALPHIDAMSLHLYPDADSWGKTPEWGTDWIVKHTLKAKKLRKAVYLGEFGTKSKDIR
                     NPVYFEWTRALFYSGANGGLYWILSDVDDNGAPYPDYDRLTVYCPSPVCTTMSNFSAR
                     MATGRILGFPPVADDDAAEGAFDSSVTLTPTSNDVGYLGRRPVPSTIDLDPSSIGQQT
                     MLTSAAGTFLLANGDVTFTPVAGFQGDAQASYTVRDTSGRKSNVAQLRAKILPDPNGA
                     ILLYSFENDAQGWAAASWQTDAGTTARVTDFATQGAASLQVTATGGGWFGLNVAEEVL
                     DISGKTQFKYDLRTLASGTSVNIAIQDRNWLWCEGSWGWQDPGTTATVAFDLASLTGN
                     GAPCDVANFSDVNAIWVNVGAGASVNIDNVRAE"
     gene            3546..4052
                     /locus_tag="VFR82_04940"
     CDS             3546..4052
                     /locus_tag="VFR82_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500977.1"
                     /translation="MKRWLFEFGFGPEATVVRGRKFVHPETAITDAEILGVWESVSFP
                     DTLIEDLNSRLHKPQMITTHQMRWAGGIDTYVDVFFEQDRLANVTAAIDVRAPHLAFV
                     SDLCLVANRHCWLAIMPNGRFFRPCVRRFMAEIQHSPATRWIRGSLDSLVQAHLSLDA
                     PGAASNPG"
BASE COUNT          874 a         1245 c         1287 g          856 t
ORIGIN      
        1 ttttgcctcg ggctttcgag cggcttcctg gagccgctcg agtcgacatc catctatctg
       61 gttcaacgcg gcctccagta tttgttgagc agcttccccc aggctgtgcc gaatcccgcc
      121 ttgcaggaga acgtcaaccg ccggaaccgt gcgcactggg atcacatccg cgacttcatc
      181 gtcctgcact acaaaatgaa caagcgcgag ggcgagccat tctgggacga gtgccggaat
      241 atgccgatcc cggaaagcct ggcggagagc atcgaattat tccgcgcgac gggccgattc
      301 acaaaggatg cgacggaatt cttccgtccc agctcctggc tttcgatgta cgcggggttc
      361 ggccttcttc ctcgttacta cagtccgtac gtcgacgacg tgccggatgc gcaactcgtc
      421 aaggaattcg agagcatggc ggcgggcatc aaaaacgcgg ttaccggcgc gcccacgcac
      481 gaggaattca ttcgcgccaa ctgcgccacc attgtgccga tgcaatcggc ggccaatgct
      541 tgagccggtc gccatgccag acttccgcgt cagtccgtcg agtgccatgt cgacgtcgca
      601 cgttggcgcg gaacgattgc cggtggtctg tatcgacgac ttcagcgtca atcccgcgct
      661 gctggtggca atcgcctgcg aagcggtgtt catcgacgtg ggttcccgat accccggcgt
      721 gcgtgcgccg gcgccccagg cctatacgaa tgcgttgctc agcgccacgg ctgcaaccat
      781 cgaacaggcg ttcggggcgc cacctgaggc agatctcgac ctgtgcgcgt tctcgatggt
      841 caccactgcg cccgaaaagc tgcggcccat ccagcgcatc ccgcatttcg acgggcccga
      901 tcgcagccga attgctttca ttcactatct atgcgcgcct taccagggcg gcacctcgtt
      961 ctatcgccat cgggcaaccg gcctggccga gatcacgccg gacaatgtgg agcgatatcg
     1021 cagcaaggtg gtcgatgagc tgaaggccga ttcgccggta caagcctatg taattgatca
     1081 cacgcatttc ttcgaaagac ttcatggagt cgaggccgca ttcaatcgac tcattattta
     1141 caaagggaat gcgcttcatt ccggcgacat ttctgcgcgc acgatattga gcgaagaccc
     1201 aagacaaggg cgcctcacta tcaacggatt tgggtttctg cgtccgagca gtcattgaca
     1261 cgtatgaggg gaaacacgat ggaacgaaca aaagtttttc accgcgctcg ctggatctgg
     1321 ctgctctgcg ccgctggaat gtttgcgctg gctggcagca aggtcacaac ccaagaccgc
     1381 aaccacgatc gcaaggaaga ccggaaccac gattacggcc atgatcgcaa ggacgatcgg
     1441 aatcgcgacc atgatcgcaa agacgaccgg aatctcggcc acgatcgtga tcacgattcc
     1501 gatcgcaagg ggcgaagctc gtttgttacc cgagagggat ccgatctcta tcttcagggt
     1561 aggctgtttc ggttcgcggg atccaacaat tactacccta tctacaagtc gcccttgatg
     1621 gtcacggctt tgctggacaa agcggcggtc agcgacttcg cagtgatgcg cgtttggtcc
     1681 accgttgtca tcggcaatca ggatggcagc aattccgtcg atggaatcaa ggaaggcgtc
     1741 tacttccatt actgggacgg cgccgccccc gccttcaatg acggcccgga tggcctgcag
     1801 cgcctcgact ttgtggtcgc cgaggccgcc aaacggaaaa tcaaactggt cctgccgctg
     1861 gtgaacaact ggaacgaatt cggcggcatg aaccagtacg tgcgctggcg cggtggccag
     1921 tatcacgacg agttctatac ggacgcgacg atcaagggct ggtacaagga gtggatctcg
     1981 cacctgctca atcgcaagaa ctcgatcacg ggcctcaggt acaaggacga cccgacgatc
     2041 atgatgtggg agctggcgaa cgagccgcgc tgtattggca gcggcggcgc caacggctat
     2101 ccgcgttctc ccaactgcac caccgacacg ctcgtctcct gggctggcga gatgtcgaaa
     2161 tacatcaaga gcatcgacaa gaaccacctc gtgtccgtgg gcgacgaagg gttcttgtgc
     2221 ctgcccaatg gcacggactg gaccgacaac tgcagcgagg gtgtggacag ttacgctttt
     2281 gcggccctgc ctcatatcga cgccatgtcg ttgcacctct atccagacgc ggattcctgg
     2341 ggcaagacgc cggagtgggg taccgactgg atagtcaagc acacgctcaa ggcaaagaag
     2401 ctgcgtaagg ccgtctatct gggcgagttc ggcacgaagt cgaaggatat tcgcaacccg
     2461 gtgtacttcg agtggacgcg cgcactgttt tacagcggcg ccaacggcgg gttgtattgg
     2521 attctctcgg atgtggacga caacggtgcg ccctatcccg actacgatcg cctgacggtg
     2581 tattgcccga gcccggtgtg cacgacgatg agcaacttct cggcccgcat ggcaaccggg
     2641 cgaattcttg gattcccccc tgtcgcggat gatgatgctg cggaaggagc ctttgactcg
     2701 agtgtgacgc ttacaccgac gtcgaacgat gtgggctatc taggtcgcag acctgtgccg
     2761 tcgacgatcg atctcgatcc ttcctcgatc ggccaacaga ccatgctcac gagcgcggcg
     2821 ggaaccttcc tgctcgcgaa cggggatgtc accttcactc ccgtcgctgg cttccagggc
     2881 gacgcgcaag cgagttacac ggtacgtgat acgagcggtc gcaagtcaaa cgtggcgcaa
     2941 cttcgcgcca agatcctgcc ggatcccaat ggggcaatcc tgttgtattc cttcgagaac
     3001 gatgcgcagg gttgggccgc cgcaagctgg cagacggatg cgggtactac ggcgcgcgtg
     3061 accgacttcg cgacgcaggg cgccgcaagc ttgcaggtga cggccaccgg cggcggatgg
     3121 ttcgggctca acgtcgcgga agaagtgctc gacatctcgg gcaagacaca attcaagtat
     3181 gacctgcgca ctttggcttc cggtacctca gtcaacattg caatccagga tcgcaactgg
     3241 ctctggtgcg agggtagttg gggttggcag gacccgggta cgacggcgac cgtggcgttt
     3301 gatctcgcat cgctaacggg caatggcgct ccctgcgacg tcgcgaactt cagcgacgta
     3361 aacgccatct gggtaaatgt cggcgctgga gcatccgtca acatcgataa cgtgcgagcg
     3421 gaatagagtc ctgaagacct ggcttcgcgt ccgttctaac ggcgcgaggc cagtgctctt
     3481 ggcgcgccgg gtgcgaacgt gcagggttgt cgccgcgaac tggcccgcat ccgtcagagg
     3541 tagcagtgaa gagatggctg ttcgagtttg ggttcgggcc agaggccacg gtagtgcgcg
     3601 ggcggaagtt tgttcatccc gaaaccgcga ttacagacgc ggagatttta ggcgtgtggg
     3661 agagcgtcag ctttcccgat acgctgatcg aagatctcaa cagccggctg cacaagcccc
     3721 aaatgattac cacgcatcaa atgcgttggg cgggcggcat tgatacttat gtggatgtct
     3781 tcttcgaaca agacaggttg gccaatgtca cggccgccat cgatgtccgc gcgccacatt
     3841 tggctttcgt atccgacctt tgtctggttg ccaaccgtca ctgctggctg gcgatcatgc
     3901 ctaacgggcg atttttccgg ccttgcgtgc gccgcttcat ggcggagatt cagcattcac
     3961 ctgccacacg ctggattcga gggtcactgg attctctcgt gcaagcacat ctcagcttag
     4021 acgccccagg ggctgcaagt aatccaggtt agccggactt ttctgtttac aaaaggggga
     4081 atcatgcaag ggcaaggagc gaacgctggc gggggacggg cgactaatag gatgattctg
     4141 tctggtaccg cgagcctcgt tctgggggtg acgcttgcgg catgtgggac gcctccgggc
     4201 acgcacacgc gcacagtgag tttcgacagc ggcgacattc gactcgcggg ggcacttgta
     4261 ac
//
LOCUS       DASTQY010000152         4358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_786800, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000152 DASTQY010000000
VERSION     DASTQY010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786800"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(430..1224)
                     /locus_tag="VFR82_04945"
     CDS             complement(430..1224)
                     /locus_tag="VFR82_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU4500978.1"
                     /translation="MKSALSILFGVAAVAFVAAPVTSFAGGTIAGKVTYAGKPEKKEF
                     SFAKFPNPKFCPKNPHKELIDGDKRFMPTIEVGKDGGLKNAVVAVVDVEDKAFMDGYK
                     GTEVVAEFCEFLPYSGVVVNNKSFKVENHDSDPDDPKSTQGVLHNPHSFTVKGSTSAT
                     GFNIGLAKKGDKLDKPVVFRGGAEKDGFYRLQCDQHEFMQSFFLPVSSPHFAVVKEDG
                     SFEIKDVPPGKHKVVAWHPFVGKGKKTEFEVEVADGGTANLKAELK"
     gene            complement(1430..2788)
                     /locus_tag="VFR82_04950"
     CDS             complement(1430..2788)
                     /locus_tag="VFR82_04950"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU4500979.1"
                     /translation="MTRRAMTWTTLGVLLFFIMGAGSSPVPPDLNTWYDQKEYQKVLD
                     GLSKLSQDAQALSDIRRLKVRTLVKLGNPQEALVEYGKLEAGLGQDDVPLLRQVAFGF
                     ILALVKDMREQMRGAAYTALKEIDSDEVVPYFEDGLSDGSGPVRVLAVEGLARSDAGR
                     KSKKLRNALEDQAGVVKARAVKALGKSGDRSVLPLIEHATKDELATVRIAAWGALIRL
                     GRTEAWEHLRKAAEAANPEERAEALRTMADLMDMRAVPLMHSLLNYNQPSVRAAAARG
                     LGHLHNQEAREDIEKLLEDPVAPVRESAAAALADLGAKESVLALTRKLSDGAFSVRAA
                     AGAALLQLSQPFDSVGPTMVALAQQNDPAARSSAALALSKATKTNRAGAVAVLTGLAT
                     DPLPGPRIVALRSLGRIGDQSVVPDLKAGLHDQNEAAKATAAGALLHILPPPKIGANR
                     RP"
     gene            complement(2792..3427)
                     /locus_tag="VFR82_04955"
     CDS             complement(2792..3427)
                     /locus_tag="VFR82_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500980.1"
                     /translation="MWTSRTWKFQALWLVFIVNVLTEAPIAPASAGTKTSPPHEFSGS
                     ITKQVNGKKYQAQVFAKTDRLRLEYKYALRTDYGYASIEIIRLDLAEVWYLLAQQKEL
                     LVTPLDPNDVLPMQPALPGEHSRTLIGDATAVERAAQLYEVQTDRHGRAERWYEWVDV
                     ETGVVLKLVSRDRDWSFAYERIRWSPQPSQYFNEPLGYQKRQVPAAQGQRG"
     gene            complement(3504..>4358)
                     /locus_tag="VFR82_04960"
     CDS             complement(3504..>4358)
                     /locus_tag="VFR82_04960"
                     /inference="COORDINATES: protein motif:HMM:NF026037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU4500981.1"
                     /translation="ALGYDVIDVEHGGTIEGTVSLEGAVPEPKGFNLITFPDPQYCGR
                     ISNGRGWRLLHDFVIGQNAGLKDAIVLLEGVEAGKPFEVSVPLIEARDCMFQPFMTIV
                     RNGHAVEVINMDPVMHDIQGYETSIEAGARVLFNTPLVMNHQHNRGDLHAIHNHAPGK
                     SLVGPIYLNKGRRTFYMQCGFHAYMESWAMAVNNPYYALTDENGKFSLEQVPPGTYQL
                     IVWHPQTGPGLTKTVTVKADGKLSEQLSLPAPKGNRSAYKVLENPRFGPESLGRSIEI
                     DPFVEHQH"
BASE COUNT          893 a         1297 c         1198 g          970 t
ORIGIN      
        1 tccccctaca gcagaatgtt gaaaaggtcg tccggcatag ttctcgcgtc gctcagtgcc
       61 tcatcatgcc gagccgggcg agagctgctc cggccgctcg gggcgggcgg gtgagaaggt
      121 ttacgcctcg cctaagacca actcgcaccc gtccgtaaat gcgacgctca ttattcttcg
      181 cgtcgcggac ctcactgcca ccccccttgc gggaggactg tctcggcgcc tcggagcggc
      241 cggtggaacc gtcggtgttt aagaacaccg tgcggccaaa atactctttt aaagacggca
      301 tcttacctag tttgaaaacg gaaacgcaag gcgcgatgcc ctgctgtcgg gagagaaaaa
      361 acaaaaggca gcggatcact ccgctgcctt ttggattgcg agttgccccg caaaaaaatc
      421 agacgtggct tacttgagtt cggccttcag attggccgtt ccaccgtccg cgacttcaac
      481 ttcgaattcg gtcttcttgc ccttgccgac aaacggatgc caggcaacaa ccttgtgctt
      541 gcccggcggc acgtccttga tctcgaagga cccgtcttct tttaccacgg caaagtgcgg
      601 gcttgaaacc gggaggaaga acgactgcat gaactcatgc tggtcgcact gtaagcgata
      661 gaaaccatcc ttctcagcac cgccacggaa caccaccggc ttgtccagct tatcgccctt
      721 cttggcgaga ccaatgttga agccggtcgc agacgtggaa cccttcaccg taaagctgtg
      781 cggattgtgc agcacaccct gggtggattt cggatcgtcg ggatccgaat catggttttc
      841 gaccttgaac gacttgttgt tgacgaccac acctgagtac ggcaaaaact cgcagaattc
      901 agccacgact tccgtgccct tatacccgtc catgaaggcc ttgtcttcga catcgacgac
      961 agcgacaaca gcgttcttca agccgccatc tttgcccact tcgatggtcg gcatgaaccg
     1021 cttgtctccg tcgatgagct ccttatgggg attcttcgga cagaatttcg ggttcgggaa
     1081 cttggcgaag gagaactcct tcttctcagg cttcccagca tacgtgactt tgccggcaat
     1141 agtaccgccc gcaaaggagg tgacaggtgc cgcgacgaag gcgacggctg ccacgccaaa
     1201 taggatggat aacgcactct tcatgttact gcctccttgt gattaacgaa agcccgacaa
     1261 tattattgat tcctagctcg ctgtcccccg tcttcattcc ggttacgaga ggaacgttag
     1321 aacgctgccc tccctgggta ctcgattgac gacttggctg gcgaagctgg tcgtagacgg
     1381 agtacatata ctgaatgtca ctgtgtggtg tcaaccagtt gcaagctcgc tatgggcgcc
     1441 tgttggcacc tattttgggc ggcggtagga tatgcaacag tgcaccggcg gcggtggcct
     1501 tagccgcctc gttttgatca tgaaggccgg ccttcaggtc cgggacgacg gactggtcac
     1561 cgatacgccc caacgagcga agcgccacaa ttctgggacc tgggagcgga tccgtcgcaa
     1621 gacccgttaa tacagcgact gcacccgcgc gattggtctt cgttgcctta ctgagagcaa
     1681 gggccgctga ggatcgtgcc gccgggtcgt tctgttgggc aagtgccacc atggtgggtc
     1741 cgaccgaatc aaacggctgg cttagttgca gcaacgccgc gcctgctgca gctcgcacgc
     1801 tgaaggcgcc gtccgagagt ttgcgcgtca gggctaagac tgattctttt gctccgagat
     1861 cggcaagcgc ggcagcggcg gattcccgaa ccggtgccac tggatcctcg agcagctttt
     1921 caatgtcttc tcgcgcctct tggttgtgaa gatgcccgag tcctcttgcg gcagcggcac
     1981 gaacggacgg ctgattgtaa ttcaagagac tgtgcatcaa cgggacggcc cgcatatcca
     2041 tcaaatctgc catcgtgcga agggcctcag cccgctcctc tggattggcc gcctcagccg
     2101 ccttgcgcaa gtgctcccag gcctccgtgc gcccaagccg gatcaaagcg ccccaggcgg
     2161 caatgcggac ggtcgccagt tcatccttcg tggcatgctc aatcagcggg agcaccgacc
     2221 ggtcgccact cttaccgagc gccttcacgg cgcgtgcttt gacaactccc gcctggtcct
     2281 ccaacgcgtt tcgcagcttc ttggattttc tgcctgcgtc cgagcgtgcc aatccttcga
     2341 cagccaatac ccggacggga ccggagccat cactcagtcc gtcttcgaaa tacggcacga
     2401 cctcgtctga atcgatttcc ttcagcgcgg tgtacgcggc tccgcgcatt tgctcacgca
     2461 tgtccttgac cagcgccaga ataaagccaa acgccacctg gcgcagcagc gggacatcgt
     2521 cctgtcccag tccggcctca agcttaccgt actcaaccaa cgcctcttga ggatttccaa
     2581 gtttgaccag cgtccgcact tttaagcgac gaatgtccga taacgcctgc gcgtcctgac
     2641 tcaacttcga gaggccatcg aggaccttct gatattcttt ttggtcgtac catgtattga
     2701 gatcaggggg aacgggtgac gatcctgcgc ccatgatgaa gaagaggaga acgccgaggg
     2761 tagtccaggt catggcacgc cgagtcatcg cctatccccg ttgcccttga gccgctggga
     2821 cttgcctctt ctgatatccc agcggttcat taaaatactg actgggttgc ggagaccatc
     2881 tgatccgctc ataggcgaac gaccagtcgc gatcgcgact gacgagtttg agcaccacgc
     2941 cggtttctac atcgacccat tcgtaccacc gttccgctcg accgtgccga tcggtttgga
     3001 cttcgtacaa ttgtgcagca cgctcgacgg ccgtggcgtc accaatcagt gtccgactgt
     3061 gttcacccgg aagggcgggc tgcatcggca acacatcgtt cgggtcaagg ggtgtgacaa
     3121 gtagttcctt ctgttgggcc agcagatacc agacttctgc gagatcgagc cggataattt
     3181 cgatgctcgc gtaaccatag tccgtgcgga gcgcatattt atattccagc cttaatcggt
     3241 cagtcttggc aaacacctgc gcctgatatt tctttccatt gacctgtttg gtgatgcttc
     3301 ctgaaaactc gtgaggaggg gacgtcttgg ttccggccga tgccggagca atgggtgctt
     3361 cagtgagcac attcacgata aagaccagcc agagcgcttg aaacttccac gtacggctag
     3421 tccacataag aatgcgacta ttcggtagga ccacggcggg tcaccggccg cttggaagcc
     3481 ggctggccgg tcatgcacat gcgtcaatgc tgatgctcga cgaacgggtc gatctcaatc
     3541 gaacgcccga gcgattctgg gccgaagcgc ggattttcca agaccttata cgcagaacgg
     3601 ttgcccttcg gcgcgggcaa ggacaactgc tcgctcaatt tcccgtcggc cttcacggtg
     3661 actgtcttgg tcaggccagg gccggtctgc ggatgccaga cgattagttg ataggttcca
     3721 ggcgggactt gctcgaggga gaattttccg ttctcgtccg tcagcgcgta gtacggattg
     3781 ttcacggcca tcgcccagct ttccatgtat gcatgaaatc cgcactgcat ataaaaggtt
     3841 cgtctcccct tgttcagata gatcgggccg acgagtgatt tgcccggtgc atggttgtga
     3901 atcgcatgga ggtcgccccg gttatgctga tggttcatga ccagcggcgt attgaacagc
     3961 accctcgcgc ccgcttcgat ggaggtttcg tatccctgga tatcatgcat gacagggtcc
     4021 atgttgataa cttcgacggc atgcccattg cgcacgatcg tcataaaggg ctggaacata
     4081 caatctcgcg cttcgatcaa gggaaccgac acttcaaagg gcttccctgc ttccacccct
     4141 tccagcagaa cgatcgcgtc tttcaacccg gcattctggc cgatcacgaa atcgtgcaac
     4201 agccgccagc cccggccgtt cgaaatccgt ccgcaatact ggggatccgg aaaggtgatg
     4261 aggttgaagc cttttggttc gggaacagcg ccctcgagag agaccgttcc ttcaattgtt
     4321 cctccatgtt ccacatcaat gacgtcgtac ccgagtgc
//
LOCUS       DASTQY010000153         5131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_669662, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000153 DASTQY010000000
VERSION     DASTQY010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669662"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /gene="rplI"
                     /locus_tag="VFR82_04965"
     CDS             <1..322
                     /gene="rplI"
                     /locus_tag="VFR82_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU4500982.1"
                     /translation="SKRVAAERAKKEKLEIEAHAKKISTVTLTVEAQVGKDDKMFGSV
                     TVKDIAEGLAGQGYTVDRRKIQLPHPIKELGSFTVPVKLPREVTAAVTVRVVKKQEPE
                     TATT"
     gene            341..1609
                     /gene="glyA"
                     /locus_tag="VFR82_04970"
     CDS             341..1609
                     /gene="glyA"
                     /locus_tag="VFR82_04970"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU4500983.1"
                     /translation="MSMKDAVGSLDALQATDPEVYAAIDAEEERQRNKLLLIASENFA
                     SPAVLAAQGSLMTNKYAEGYPGKRYYGGCQHVDTVEELAIQRCKQIFGAEHVNVQPHS
                     GSQANMAAYLSVLKPGDTILGMDLAQGGHLTHGSKVNFSGMIFRAFSYGVDRQTETID
                     YDAVRKLAEDCRPRMIVVGASAYARTLDFPKFQAIAKGVGAYLMVDIAHIAGLIASGL
                     HPNPIPHADFVTTTTHKTLRGPRGGVVMCKAEHAKAVDKFVFPGMQGGPLMHVIAAKA
                     VAFKEALSPAFKRYQQQVIANARVLAQGLLDRGYKIVSGGTDTHLMLVNLTNKGITGK
                     EADAALDAAGIIVNKNAVPYDEKPPAVASGIRLGTPIVSTRGMKEAEMKEIVDLVDRV
                     LQDRHDTAVLEQIRGRAKALCSRFPIFHSY"
     gene            1636..2100
                     /gene="nrdR"
                     /locus_tag="VFR82_04975"
     CDS             1636..2100
                     /gene="nrdR"
                     /locus_tag="VFR82_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEU4500984.1"
                     /translation="MKCPFCDELEDKVVDSRMAKEGELIRRRRECLGCKRRYTTYERV
                     EEILPVVVKKDGRRETFDRSKILSGLKKACEKRPISTATIEAVADRIEKHIQEMGETE
                     IESRVVGEELMKELHQLDQVAYVRFASVYREFKDIDQFMDELRTLAQQRRER"
     gene            2188..3297
                     /locus_tag="VFR82_04980"
     CDS             2188..3297
                     /locus_tag="VFR82_04980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU4500985.1"
                     /translation="MPASQTKLSKQIRKNGVAGVTHVAIIGAGHGGTALLEIFANDPL
                     VDIVAVAEIDASAPGLALAKRFKIPVTRDYRMLLNLERVDLIIDVSGSPDVWQSLQDF
                     HRMGVTILGGASAKFMWELIEARIRATAEIEKTLNKYQSLYRLYVKETGAAVTEERTR
                     IACEIHDGLVQSLAGVGFKLELSQQLIRKDPKASLATLRESKEQLKHAIHEARQVIFN
                     LRPLQYDKMELIPGLTNYLKSYETQYHIKTEFTVSGDEQILFPRTKIFLFRIVQEALS
                     NVVQHAKADRVSVRLTIDLDILRVTISDNGVGFDMQHVLHNPAKWDHFGVRGILERAK
                     LVGGEAKIDSKKGRGTKIVVEVPLGKKEHSDDGKN"
     gene            3284..3955
                     /locus_tag="VFR82_04985"
     CDS             3284..3955
                     /locus_tag="VFR82_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4500986.1"
                     /translation="MEKIKVLIADDHRVVREGLAAILKTKDDINVVGEAQDGMEAVEK
                     TKTLMPDVILMDVSMPRMGGVEATRQIKREFPHIGIVALTMYEEQQYIFDLVRAGATG
                     YLLKDSESSQIVAAIRAIYRGESLIHPSVASKILAEFSLMAQKKGKKPAWVEHDLTER
                     EITVLRLVADGKTNKEIANSLDLSEKTVKNHVRNIFHKLQVYDRTQAAILAIRKGLIE
                     LDPRP"
     gene            4347..>5131
                     /gene="lpxC"
                     /locus_tag="VFR82_04990"
     CDS             4347..>5131
                     /gene="lpxC"
                     /locus_tag="VFR82_04990"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEU4500987.1"
                     /translation="MRLQQTVANPVSCRGVGLHTGKPVTLTIRPAPADTGVVFVNRNG
                     HAGASLAASVDHLVATELCTAISGNGFQVKTIEHVLAALAGLNVDNAYVDIDASEAPV
                     MDGSSAPFVRLIESAGVVSQNRRRSFLKITRPLEIVDGSRRIRIEPSSTPRITYTIHY
                     DHPLIQTQTYAYEHSSRAFASDIAGARTFGFLQEVEALWARGLGRGGNLENTVVLSQD
                     GILNESGLRFTDEFVRHKVLDLIGDFSLLGLPFIGHLIADRSG"
BASE COUNT         1216 a         1395 c         1448 g         1072 t
ORIGIN      
        1 tagcaagcgt gtggcagctg aacgggcaaa gaaggagaag ctcgagatcg aagcgcacgc
       61 gaagaagatt tccactgtca ccctgacggt cgaggctcag gtgggcaagg acgacaagat
      121 gtttggctcg gtcacggtca aagacattgc cgagggtctt gcggggcaag gctacacggt
      181 cgatcgccgc aagattcagc tgccccatcc catcaaagag ctgggcagct tcaccgttcc
      241 ggtgaagtta ccgcgtgagg tgaccgctgc agtcaccgtg cgcgtggtga agaagcagga
      301 acccgaaacc gctaccacgt aacaccaacg aggcgccttc atgtccatga aagatgcagt
      361 gggctccctt gatgccttgc aggccaccga tcccgaggtc tacgccgcca tcgatgccga
      421 ggaggaacga caacgcaaca agctgctgct gatcgcttcg gaaaacttcg ccagtcccgc
      481 cgtgttggcc gcgcaaggct ccctcatgac gaacaaatat gccgagggtt atcctggcaa
      541 acgctattat ggcggctgcc agcatgtgga cacggtcgaa gaactggcca tccaacgctg
      601 caaacagatc ttcggtgctg agcatgtaaa cgtccagcca cactccggct ctcaagccaa
      661 catggcagcc tatctctccg tgctcaagcc gggggatacg atactcggca tggacctcgc
      721 gcagggtggc cacttgaccc atgggagcaa agtgaacttc tccgggatga ttttccgcgc
      781 cttttcttac ggtgtcgatc gccaaacgga aaccatcgac tacgatgcag tccgcaagct
      841 ggcagaagat tgtcgtccac ggatgatcgt ggtgggtgcc agtgcctatg caaggacgct
      901 cgattttcca aagttccaag ccatcgcgaa aggggtcggc gcctatttga tggtcgatat
      961 cgctcacatt gccggattga ttgcctcggg tttacatccc aatcccattc cccatgcgga
     1021 tttcgtgact accacgacgc acaagaccct ccgggggccg cgcggtggag tggtcatgtg
     1081 caaggccgaa cacgcgaaag ccgtcgacaa gttcgtcttt cccgggatgc agggcggtcc
     1141 cttgatgcat gtgatcgcgg ccaaagccgt cgcgttcaag gaggcccttt ctccggcgtt
     1201 caaacgctat caacagcaag tgattgccaa tgcccgtgtg ttggctcagg gcctgctcga
     1261 tcgaggctac aagattgttt ccggtggtac ggacacgcat ctgatgctgg taaatctgac
     1321 gaacaaggga attaccggca aagaggcgga tgccgcgctc gacgcggccg gcatcatcgt
     1381 gaacaagaac gccgtgcctt atgacgaaaa gccgcctgct gtagccagcg gcatccgcct
     1441 cggcacaccg atcgtgtcga cccgcgggat gaaggaagcc gagatgaaag agatcgtcga
     1501 cttggtcgat cgggtcttgc aggatcgaca cgacacagca gtgctcgagc agatccgcgg
     1561 ccgggcgaaa gcgctgtgta gccgttttcc gatttttcac tcctactaac ctcacgcagg
     1621 gcaggatcac cgccggtgaa atgtcccttc tgcgacgagc tggaagacaa agtagttgac
     1681 tctcgcatgg cgaaggaggg ggaactcatc cgacgccggc gagagtgtct cggatgcaag
     1741 cgtcgttaca ccacctatga gcgcgtcgaa gagatccttc ccgtcgtggt caagaaagac
     1801 gggcgccggg agacattcga tcggtctaaa atcctttcgg ggttaaagaa ggcttgcgaa
     1861 aagcgcccaa tcagcacggc gaccatcgaa gcggtggccg accggattga aaaacacatt
     1921 caggaaatgg gcgagaccga gattgagagc cgcgttgtcg gcgaggagtt gatgaaggaa
     1981 ttgcaccaac tcgatcaagt cgcctatgtg cgctttgcgt ccgtatatcg tgaattcaaa
     2041 gacatcgacc agttcatgga cgagctgaga accttggccc aacaacgtag ggagcggtga
     2101 gtacgctgcc cgcttgctcg cttggacgtc ttttctctcc tcccgctcgt ctagaccata
     2161 cgacgctcca acagtgatag agggcacatg ccggcctcgc agaccaaact gtccaaacag
     2221 atcaggaaaa acggtgtcgc cggcgtcacg catgtcgcca tcatcggcgc gggtcatggt
     2281 ggtactgcgc tcctcgagat atttgccaac gatcccctgg tggacatcgt ggccgtggcc
     2341 gaaattgatg cctccgcgcc tggccttgca ttggctaagc gattcaagat tcccgtcacg
     2401 cgggattatc gcatgcttct caaccttgag cgcgtggatc tcattatcga tgtctcaggc
     2461 agcccggatg tctggcaatc tctgcaagat tttcaccgca tgggcgtcac gattctaggc
     2521 ggggcaagcg ccaagttcat gtgggagttg atcgaagccc gaatcagggc aacggccgaa
     2581 atcgagaaga cgctgaacaa atatcaatcc ctctaccgct tgtacgtcaa ggaaacgggc
     2641 gctgccgtga cggaagaacg gacgcggatc gcctgtgaaa tacatgacgg actggtccag
     2701 agcctggccg gcgtgggatt caaacttgaa ctctctcaac agttgattcg caaagatcca
     2761 aaggcaagtt tggcgacgct gcgcgagagc aaggagcagc tcaagcacgc gatccatgaa
     2821 gcgcgtcaag tcatcttcaa tctccgtccg cttcaatacg acaagatgga gcttattcca
     2881 ggactcacca actacctgaa gtcctatgag acgcaatatc acatcaagac cgagtttacg
     2941 gtttcaggag atgagcagat tttgttcccc cgcacaaaga tctttttgtt tcgtatcgtg
     3001 caggaggccc tgagcaacgt cgtccaacat gccaaggccg atcgtgtgtc cgtacgactg
     3061 acgattgatc tggacatcct cagagtcacc atttcagaca acggcgtcgg cttcgacatg
     3121 caacatgttc tccataaccc ggcaaagtgg gaccatttcg gcgtacgagg gatcctcgag
     3181 cgggcgaagc tcgtcggagg cgaagcaaaa atcgattcca agaaaggccg gggcacgaag
     3241 atagtggtcg aggtgccgct cggaaagaag gagcatagcg acgatggaaa aaattaaggt
     3301 actcattgca gacgaccatc gcgtggtacg ggaaggcctc gcggccattc tcaagaccaa
     3361 ggacgacatc aacgtcgtgg gcgaggcgca ggacggaatg gaggcggtcg agaaaaccaa
     3421 gacgttgatg cccgacgtga tactcatgga cgtcagcatg ccacggatgg gcggtgtgga
     3481 ggcgacccgt cagattaaac gcgagtttcc tcatatcggg attgtggcgc tgaccatgta
     3541 tgaggagcag caatatattt ttgatctggt ccgcgcgggc gcaaccggct atctcttgaa
     3601 ggattcagaa tcgtcgcaaa tcgtcgctgc cattcgtgcg atctatcggg gcgagtcgtt
     3661 gatccatcct tccgtcgcca gcaagatcct cgctgagttt tcgctgatgg ctcaaaagaa
     3721 aggaaagaag cccgcctggg tggaacacga tctcactgag cgcgaaatca cggtcctgcg
     3781 cctcgtcgcg gacggaaaaa ccaataagga gatcgcgaac agtctcgacc tcagcgagaa
     3841 aacggtcaaa aatcacgtcc gaaatatctt tcacaagctg caggtgtatg acaggacgca
     3901 ggccgcgatt ttggcgatcc gtaaggggtt aatcgaattg gatccacgtc cgtgatattg
     3961 cattcaaccc tctcacggtt aactggattc gttcgtggag gcggtcgccg aagttgccca
     4021 ggaggtaaag aaagagaggc cgatgcgtcc ctccgtattg caaccgttgt cctcatcgcg
     4081 gtaaacttga gacttgagtt agtcacgctt gtcgtccctc tgagaccctg aatccgagag
     4141 cgagcgtaaa tccccagccc attcgaccag cctctcccgg gaaacgcgct tatgaaagtc
     4201 acacctgtca catttaagcc acaacactat aggatgacgg cttattataa attcatttta
     4261 ttatcaattg cttatgatga ttcacactta taacttctgt tggcattaga gttgctgaag
     4321 gtccaaggtg aagcggaggc tgctcagtga gactgcaaca gaccgtcgca aatccagtga
     4381 gctgtagagg cgtagggctt cataccggca agcccgtcac gttgaccatt cgaccagccc
     4441 cagctgatac cggagtcgtg ttcgtcaatc gtaatggaca tgctggggcc tcgctggcag
     4501 cgtctgtgga ccatttagtg gctaccgaac tctgtacggc tatcagtggg aatggattcc
     4561 aggtcaaaac cattgagcac gtcttagcgg cgctcgcggg cttgaacgtc gataatgctt
     4621 atgtcgatat cgacgcaagc gaagcaccgg tcatggacgg tagttcagcc ccctttgtcc
     4681 gattgattga atcggcaggt gtggtgtctc agaaccgacg ccgatccttt ttgaagatta
     4741 caagacctct cgagatcgtc gacggctcgc gacgcatccg gattgaacca tcttccaccc
     4801 cgcgcattac ctatacgatt cattacgacc atcccctgat tcaaactcaa acctacgcgt
     4861 atgaacattc ctcccgtgcg ttcgcgtctg atatcgccgg agccaggacg tttggattct
     4921 tgcaagaagt ggaagcgctg tgggcccgcg ggttaggtcg tggggggaac ctcgagaata
     4981 ccgtggtcct ttcgcaggac gggattctca atgaatccgg tcttcgtttt accgacgaat
     5041 tcgtccgtca caaagtgcta gatttgattg gtgacttctc gcttctgggg ctgcccttca
     5101 ttggccattt gattgcggac cgctcaggac a
//
LOCUS       DASTQY010000154         3839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_40749, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000154 DASTQY010000000
VERSION     DASTQY010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40749"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VFR82_04995"
     CDS             complement(<1..882)
                     /locus_tag="VFR82_04995"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU4500988.1"
                     /translation="MTTRSLPRTPTRTTRSGKRNSKTKEDGPASGQQLRAVSDRLGGN
                     GNGSLTQEERLAYGLGWFGIGLGLTELLMPRQLAHATGVPIRHRRLIRIMGLREIASG
                     LGIITQRRPAGAVWSRVLGDVIDLACLGVAFTSAGADRRKLATAVTAAAGATALDLLC
                     AQQLTRGVETRNGAIAVTVGLIIDRSPGDLYSYWHDFTNFRSFMKHVISVEVTGERRS
                     HWVVTGPAGSTVEWDAEVTEEVPDTLIAWRTLEGADVDHRGQVRFEAAPGKRGTLVTV
                     DMQYHPPAGTLGSAIATW"
     gene            complement(969..1478)
                     /locus_tag="VFR82_05000"
     CDS             complement(969..1478)
                     /locus_tag="VFR82_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500989.1"
                     /translation="MNGPRLVRYIWACMLLQAVPAFCQDAILPQGDPRLRDDPQQAEQ
                     REEVRDSRMKQQGQGPDYRVEGKPQGGTRGATAGEPNGRTRQDTGLSDPSVNPGQASG
                     MRVLRGEVKQANPKSIVIEDRQGKETTMSVDAATAGDRDIRPGDFITGTVTTQGRAVA
                     IHKETPPER"
     gene            complement(1475..1999)
                     /locus_tag="VFR82_05005"
     CDS             complement(1475..1999)
                     /locus_tag="VFR82_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500990.1"
                     /translation="MARIVFRQLRTPAVPCSHGWVSSRTIASGCVSGVTVAAAAAWRA
                     HAEGQSGWKAINGISHILWGPQAAVQRRFTAKFTGSGLVLNAVACGFWGWLYGVIDQF
                     TPAPHSYSQSAGRGIAVAALAYVTDYYLIPRRFTPGFELSLSRRSFPWLYGALAVGLI
                     LPDCLALSKSRSRP"
     gene            complement(2005..2454)
                     /locus_tag="VFR82_05010"
     CDS             complement(2005..2454)
                     /locus_tag="VFR82_05010"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU4500991.1"
                     /translation="MSVIEKSIEINVPVRAAYNQWTQFEEFPKFMEGVKQVKQIDDKH
                     LYWKADIGGKEKEWKAEITEQIPDERIAWTSRGGALNAGVVTFHRLSDSKSKVMLQME
                     YDPQGFVENVGDAVGVVTQRVQGDLERFKQYIENRGQETGAWRGTVT"
     gene            complement(2492..2734)
                     /locus_tag="VFR82_05015"
     CDS             complement(2492..2734)
                     /locus_tag="VFR82_05015"
                     /inference="COORDINATES: protein motif:HMM:NF022397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HEU4500992.1"
                     /translation="MEDQLDGEVLRRIEELVSQEHGFQEQELLSDDDRARLRGVQVEL
                     DQSWDLLRQRRALRAAGQDPNRAQVRPAGIVQKYVQ"
     gene            complement(2766..>3839)
                     /locus_tag="VFR82_05020"
     CDS             complement(2766..>3839)
                     /locus_tag="VFR82_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500993.1"
                     /translation="KLSSGMTWDQLAQLSPEEIKKKGAFPPGFDRLPHVKHAVGGQVF
                     PQVQIKEFPRLERFDVDFDLPECLLPEFPPPIFLTTHPELGDVSQGEVVHAENFDRLF
                     RGLVTPAQLDGLRMLVMQFAQEEFNATHDRKTAKPSLGVSCLDCHVNFHTTGQFHLNP
                     DTRPQMERLRLDTVSLRGMFNQQIHGSKRSIRSVEDFTEFEQRTAYFNGDPIRAMKKG
                     MNIIDRVPVAHMAQMQNMIDFPPAPKLDPLTGRLDRAKATPLETKGEDLFFGKAKCAA
                     CHRPPAFTDHQMHDLHLERFGAAADGPIKTFVLRGIKDSPPYFHDGRLPTLEDVVEFF
                     NVIGGLKLDPDEKKALTAFMRAL"
BASE COUNT          750 a         1172 c         1065 g          852 t
ORIGIN      
        1 ccaggtggca attgcggagc ctagcgtgcc tgccggtgga tggtactgca tgtcgacggt
       61 gaccagcgtg ccccgttttc ccggtgccgc ctcaaaacga acctggccac gatggtctac
      121 atccgcaccc tcgagggtgc gccacgcaat cagcgtatcg gggacctctt cagtgacctc
      181 ggcatcccat tcgacggtgg agccggcagg accagtcacc acccaatgtg atcgacgctc
      241 gccagtgact tcgacggaga tcacatgctt catgaacgac cggaaattgg tgaagtcgtg
      301 ccaatagcta tacaggtctc ccggcgagcg gtcgatgatg aggccgacgg taactgctat
      361 cgccccgttg cgggtttcca cccctcgggt gagttgctgc gcgcagagca gatcgagtgc
      421 cgtcgctccg gcggccgcag tgacggcagt cgcgagtttt ctgcgatcgg cgcccgcgga
      481 ggtgaacgcg acacctaggc aagccaaatc gatcacgtcg ccgagcaccc gagaccagac
      541 ggcgccggcc ggtctacgtt gggtgataat tcccagcccg ctcgcgatct cgcgcaaccc
      601 cattatccgg atcagacgac gatgtcgaat gggtaccccg gtcgcgtgcg ccagttgtcg
      661 aggcatcaag agttccgtga ggcccagtcc gattccaaac catccgaggc cataggccaa
      721 ccgctcttcc tgggtaaggg atccgttgcc gtttccgccc aagcggtcac tgacagcgcg
      781 cagctgctgg ccgcttgcag gcccgtcttc ctttgtcttc gaatttctct tgcctgaccg
      841 tgtggttctc gtgggtgttc gtggaagaga tctcgtggtc atcgcatctc cttgtcttcg
      901 tttctgaagg tcgatatcga gtgctcaaca gccattggcg gtggagcgga ggtgtttcgc
      961 tgcatctgct acctctcggg tggcgtctcc ttatgaatag cgacggcgcg gccctgcgtg
     1021 gtcactgttc cggtgataaa atccccggga cgaatatcgc ggtctcccgc cgtcgcggca
     1081 tccaccgaca ttgtcgtctc tttcccctgt ctgtcttcga tgacaatgga tttcggattc
     1141 gcctgcttca cttcgccacg caacacgcgc ataccagaag cctgaccggg attcacgctc
     1201 ggatccgaca ggccggtgtc ctgccgagtc ctgccgttgg gctcgccggc tgtggcgccc
     1261 ctggtccccc cttgcggctt accctccacg cgatagtccg gcccctgtcc ctgctgtttc
     1321 atccggctgt cgcgaacctc ttcgcgctgc tcggcttgct gagggtcgtc tcggagacgt
     1381 ggatcgccct gaggcaagat ggcatcctgg caaaacgcag gaacagcctg aagcagcatg
     1441 catgcccata tgtacctgac gagtcgtggt ccgttcatgg tcgactcctt gattttgaga
     1501 gtgcaaggca atcgggcaag atcaaaccga cagcgagtgc tccgtagagc caaggaaaac
     1561 tccggcgcga caggctcaac tcaaagccgg gcgtaaaacg acgagggatc agatagtagt
     1621 cggtgacata ggccaaggcc gcgaccgcaa tcccacggcc ggctgactgc gaataggaat
     1681 gcggtgcagg cgtgaattga tcaataacgc catacaacca cccccaaaag ccgcacgcca
     1741 ccgcgttcaa cacaagccca gagcccgtga acttggcggt gaaacgccgt tgtactgccg
     1801 cctgaggtcc ccagaggata tgactgatgc cgttgatcgc tttccagccg gattgccctt
     1861 ccgcgtgcgc acgccaggcc gctgctgcgg ccaccgtcac accggacacg cagccggaag
     1921 cgatcgtcct ggacgaaacc cagccgtggg agcacggcac ggccggcgtt cgaagctgtc
     1981 gaaatacaat acgagccacg gcgcctatgt caccgtccct ctccaggcgc cggtctcctg
     2041 ccctcgattt tcgatgtatt gcttgaaccg ttcaaggtca ccttgcaccc gctgcgtaac
     2101 gacccctacg gcatccccga cgttttctac gaagccctgc gggtcatatt ccatttgcag
     2161 catgactttc gattttgagt ccgacagacg atgaaacgtc accacaccgg cgtttagggc
     2221 gcctcctcta ctcgtccagg cgatgcgctc atcgggaatc tgctcggtga tctccgcttt
     2281 ccactccttt tcttttcctc cgatatccgc tttccaatac aaatgcttgt catcgatttg
     2341 cttgacctgc ttaacgcctt ccatgaattt cggaaactct tcaaattgtg tccactggtt
     2401 gtaggcggct cgcacaggca cattgatttc gatcgacttt tcaatgacgg acatgtcgtc
     2461 ctcctttcac gcgtggtgaa cgctcatcga atcattgcac atacttttga acaatccctg
     2521 ccggtctgac ttgggcccta ttggggtctt gtcccgcggc gcgcaaggcc ctccgctgcc
     2581 tcaatagatc ccaactctgg tcgagctcca cttggacgcc gcgcagtctt gcccgatcgt
     2641 catcggacaa caactcctgc tcctgaaaac catgttcttg tgagacgagt tcttcgatcc
     2701 gacgaaggac ttccccatcc aattgatcct ccatgtgtcc tctcccttgc ttttttgtcc
     2761 tggggtcata acgcccgcat aaaagcggtg agggcctttt tctcgtccgg gtcaagtttc
     2821 aggcccccga tcacattgaa aaactccacg acgtcctcga gagtgggaag acggccgtca
     2881 tgaaaatacg gtggtgaatc cttgatccct cgcagcacaa atgttttgat cggcccgtcc
     2941 gctgctgcgc cgaaccgttc cagatggaga tcgtgcatct gatgatcggt gaaggcaggc
     3001 ggacgatgac aggccgcaca cttggccttc ccgaaaaaga ggtcttctcc tttggtttcg
     3061 aggggcgtcg ctttcgcacg gtcgaggcga ccggtcaggg gatccagctt gggtgcaggg
     3121 ggaaaatcaa tcatgttttg catttgggcc atatgcgcga ctgggacgcg gtcgatgata
     3181 ttcatcccct tcttcatggc acggatcggg tcgccgttga agtaggcggt gcgttgctca
     3241 aattcagtga agtcctccac agaacgaatt gaacgcttcg acccgtgaat ttgctggttg
     3301 aacatgccac gaaggctgac cgtgtccaac cgcaggcgct ccatttgcgg tcgcgtgtcc
     3361 ggattcaagt gaaattgccc ggtcgtatga aaattgacgt ggcaatcgag acagctgacg
     3421 cccaaacttg gctttgctgt tttacggtcg tgtgtggcat tgaactcttc ctgcgcaaac
     3481 tgcatgacaa gcatgcgcag cccgtccagc tgtgcaggcg tcacgaggcc gcgaaaaagc
     3541 cggtcaaaat tttccgcatg gacaacctcg ccctgggaca catcccccag ctcgggatgg
     3601 gtcgtcaaga aaatcggtgg gggaaattcg gggagtagac attccggaag gtcaaaatcg
     3661 acgtcgaatc gttcgagccg tggaaattcc ttgatttgca cctgggggaa gacctgaccg
     3721 ccgacggcgt gcttgacatg cggcagccga tcgaatcccg gagggaaggc ccccttcttc
     3781 ttgatctctt ccggcgacag ttgagccaat tggtcccatg tcattcctga cgacagctt
//
LOCUS       DASTQY010000155        11382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_870840, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000155 DASTQY010000000
VERSION     DASTQY010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870840"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VFR82_05025"
     CDS             <1..456
                     /locus_tag="VFR82_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEU4500994.1"
                     /translation="AGDHVEIGGLGMTGKLLEPPQGKKRVRVKVGDGELLATVANLVG
                     IGRGTEALQPAPRTTRTSLGGERSYPVETPVVDVRGQAVDEAVDQVLAALDRATLEEA
                     HSLRIIHGHGTGKLKSTLRTYLQSSPYVAHLRPGERHEGGDGVTIVSIR"
     gene            complement(492..1844)
                     /locus_tag="VFR82_05030"
     CDS             complement(492..1844)
                     /locus_tag="VFR82_05030"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU4500995.1"
                     /translation="MHTPIVALVSADPTSRTVLEQALSDCGGTLLTATTTEAGVRLLA
                     TRHDITLVICDDSSGRISRAVVLAQARQIPGAVPIVVLGRQDTAKAAIEAIREGASDY
                     LPTPLSADAVSTSILNARAIRPSEDTLEDLLGFEHIISVSPQMQLVKRLAAEVAATDA
                     TVLITGASGTGKELFARAIHATSLRSKGPFVAINCAGIPEPLLESELFGYQRGAFTDA
                     KQAKPGKFQMAAGGTLFLDEIGEMSLSAQAKLLRVLEDHVIDPLGDTNSIKVNIRVIA
                     ATNEDLPAQIKAGRFRLDLFYRLNVYQLRIPPLHERPDDIEPIFSSFLDHARKHHGCR
                     IRGIAPEALSLLQRHDWPGNVRELHNIVEGLTINCKSDTIHPEHLPASFRPAKAPSPT
                     VGAEKTSMLAFGLSFHEMEKNILEEALRKAAGNVSQASRLLKITRNTLRYRMAKYHIS
                     "
     gene            complement(1946..2899)
                     /gene="queG"
                     /locus_tag="VFR82_05035"
     CDS             complement(1946..2899)
                     /gene="queG"
                     /locus_tag="VFR82_05035"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEU4500996.1"
                     /translation="MSIGQAIKREARLLGFDAVGITSVVLEPPAHGLLDRLQEWLRRG
                     YHGTMAWMARDPDRRAHPQIVLPGCRSIISVGMNYDTGLRAIEGRGHGRIARYAWGKD
                     YHDVLSNRLVLLENKVRDLVPEAITRPYVDTGPVMEKAWAQQAGLGWIGKHSNLVSSD
                     HGSWLVLGEILTTLELPPDAPGTDLCGTCSLCIQACPTNAIVEPYVVDATRCISYLTI
                     EFRGPRRDIPVDLTARMGNHIFGCDDCLDVCPFNLRAEPTKEPAFQPDATTLGPRLDD
                     LAALDEETFAGLFRHSPIRRSKLSGLQRNVAIAQENDKSST"
     gene            complement(3015..3389)
                     /locus_tag="VFR82_05040"
     CDS             complement(3015..3389)
                     /locus_tag="VFR82_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4500997.1"
                     /translation="MRVTPSLLSLGGLMLCAFLLLRPADAQGQVPSDFPATTEQACSF
                     GMAKGEPSHNCQVPIPHGCLVANAPGTNKPWTTISKGGKTFCRFDEKQTDWKTKITGT
                     CGRCSTGHCSVQFIVRFDCNPH"
     gene            3510..4622
                     /locus_tag="VFR82_05045"
     CDS             3510..4622
                     /locus_tag="VFR82_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HEU4500998.1"
                     /translation="MLATRIRQKEGTFYFIAYNAADLLDKVRFTSRYFFEGEEIAQGK
                     ISEHDDVAQFIAGIERSEKGFQRQLNRQKIKQIVNFYETVVAQPMIPGTVLIFTDETL
                     RFQRAGESESIGHLSEPKGKYLIIDGQHRLAGLHFFRAKHPDQIANVEVPCVLFDGRS
                     GDFATEMFVVINSTHTRINRSHLVDLYEKVSWETPEKKFAAKVVNLLYAEDDSPLQYK
                     INRLGGRSKQEKWILQSEVFNELLKVVIAHKRWMESHLGMKAERCYALVRDYLKGVKE
                     VMEEVWGNNDRYMFTRDVSLKALIRVLDDLIVDRKLINDWDAQRSHKPFAEIVKPWAA
                     LSKEFRAEGFYERFPAKGQLERVRKIHQRLLDAIVG"
     gene            4720..5190
                     /locus_tag="VFR82_05050"
     CDS             4720..5190
                     /locus_tag="VFR82_05050"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU4500999.1"
                     /translation="MKTDPAGGVIATARLVFPATPHIIQSLLTDYAHWPDLFEVRMRI
                     VDLQIARGVVTTDLRIDHALLPGERRLVTESRVISGVIHTDLVAGDFKRYHRVWRLAP
                     TNEGRETRAEFELSVAVDSILPDWLVALAMRRELEAHFRIVKEKARALAASSEK"
     gene            complement(5174..6352)
                     /gene="thiI"
                     /locus_tag="VFR82_05055"
     CDS             complement(5174..6352)
                     /gene="thiI"
                     /locus_tag="VFR82_05055"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HEU4501000.1"
                     /translation="MRCVIVHYHELALKGRNRDFFEQRLIHNIRSVLKDLGATRIESL
                     RSRIRIVLPEHISDQAARSRLALIFGIANFSLAHSVPLNLLKPDLGELTRGIVNAVKQ
                     RAFDTFRVTAKRADKRLPLTSMDVEKAVGKYVCDLTGKKVRLTDPGLIIYVEMLAKDA
                     YYYLDKFPGPGGLPVGVSGKVACLVSGGIDSPVAAYRMMKRGCNAVFVHFSGRPLVSR
                     DSEDKVSDLVQTLTAYQCHSKLFIVPFGEIQREIVAQAPAPYRIVLYRRLMVRIASEL
                     AQREGCWALVTGDSLGQVASQTPENLSVIEEAAEFPVLRPLIGMDKLEITDQAQRIGT
                     FTTSIEPDQDCCSLFTPSHPSTKTRMDDIRRIERSLDIGTLVKQGMNRVESSEFTFPS
                     "
     gene            complement(6375..7298)
                     /gene="trxB"
                     /locus_tag="VFR82_05060"
     CDS             complement(6375..7298)
                     /gene="trxB"
                     /locus_tag="VFR82_05060"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU4501001.1"
                     /translation="MRNVLIVGSGPAGLTAAIYAARANLAPLLVEGWQSGGQLTTTTE
                     VENFPGFAKGIMGPDLMKEMRAQAERFGTDFLTGDVSAAEFGTRPLKITVDHERTIEA
                     RTVIIATGASAIQIGLKNEARLTGHGVSTCATCDGFFFRGKELVVVGGGDSAMEEANF
                     LTKFATKVYVVHRRDKLRASKIMQDRAFKNDRISFIWNTVVEDILGEETVSGVRLNNL
                     VMGTTTTLPASGVFVAIGHRPNTALFAGQLDMDAKGYIVTKHGTATSVPGVFAAGDVQ
                     DSIYRQAITAAGTGCMAAIDAEKFLELKGHH"
     gene            complement(7328..7993)
                     /gene="coaE"
                     /locus_tag="VFR82_05065"
     CDS             complement(7328..7993)
                     /gene="coaE"
                     /locus_tag="VFR82_05065"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU4501002.1"
                     /translation="MLEAAQRLSRHPHGILRRMILVGLTGGIATGKSTVASMFRRFGA
                     TVIDADQLARDVVQPGKPAWREIVKIFGKRVLLPDRTIDRHALGSIVFHNRRKLRRLE
                     RIIHPRVAREQQRRAHGIARRTPDAIIIYEVPLLFEAGVNTRVDKIIVVTADRETQVA
                     RLKRRNGLTRRQAFQRISSQMALAEKARRADIVLDGTQSKSLLSREIRRLLRDFRAGD
                     RPV"
     gene            8038..9048
                     /locus_tag="VFR82_05070"
     CDS             8038..9048
                     /locus_tag="VFR82_05070"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HEU4501003.1"
                     /translation="MLMRTIGCLTLWSLVVCAVVHAESGSAQAPRTIVVALDGSGDFR
                     SIQEAVDAAAKGDTVFLKPGRYEQDITIHSKDHVKLIGAGQDKVTLVGREDLVGVLHV
                     GKWPYGATNIEISDMTINEHGGHAVGLFNGRGLLLKRLTVNGMLFTQQVEDTRIEDCT
                     IGGSETTGVQFADSQAVLVGNFIHDNDHGVNVAGKSNVRLERNVITRSLFEAVVVNDR
                     AKAVLVSNTLVKNGGGAAFLGQSQSDVSGNVVGLNRVGFLVASSSRAATSYNALYNSD
                     GDYLREGNPNQIAPELKAQSDITGDPYFVDPAKGDFRLRADTPLRKIGSFPFLGALPP
                     AE"
     gene            9179..9364
                     /locus_tag="VFR82_05075"
     CDS             9179..9364
                     /locus_tag="VFR82_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501004.1"
                     /translation="MASYKQELEKRGPEGIPSLDDIEPGKICGAVIPMSERAQTQMRD
                     SIEVIDYLLNQERVVWS"
     gene            9469..10017
                     /locus_tag="VFR82_05080"
     CDS             9469..10017
                     /locus_tag="VFR82_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEU4501005.1"
                     /translation="MKFLNRLRLFHSYASVFLIATGLVILVCPSFSFADELPKEAVLP
                     LAMASKAAQAAMEACKKDGYRVSVSVVDRAGVLRTMGRADGAGSHTVESSRKKAYTAA
                     SLRRATTELAELIGKVPTLQSLREMNDEILILGGGLPIEIGGDVVGGIGVGGAPGAHL
                     DDACAQAGLDAVGAAPKVPAVK"
     gene            10083..10469
                     /locus_tag="VFR82_05085"
     CDS             10083..10469
                     /locus_tag="VFR82_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501006.1"
                     /translation="MPVSAVTQVTPPGFIVSVTRIATHPFLSRFNLALTVRTAGGCAA
                     TSELFPDTGGVSRRNLYLTTSDRLYVVGQFDVRRFDPQRCLIELVEFRSVEDGLRFIG
                     SFDTDRSGQWAFQTASAQAERPFEPL"
     gene            complement(10862..>11382)
                     /locus_tag="VFR82_05090"
     CDS             complement(10862..>11382)
                     /locus_tag="VFR82_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU4501007.1"
                     /translation="GFSHRECLAFAKGVTRFGGKLVLLEQPVLREDLESLAAIRRETG
                     IPVAADESVRSLVDAKQVVDRHAADYINIKIMKTGVLEAHEIALFALGSGLKLMVGGM
                     VETRIAMGCSFSLVLGVKGFEVLDLDTPLLLDNDPVIDGYQYRGPRLVPWHRPGLDLL
                     VRPPDAIATLTR"
BASE COUNT         2420 a         3158 c         3353 g         2451 t
ORIGIN      
        1 gctggagacc atgtagaaat cggcggcctg ggaatgaccg gaaagttgct cgaaccgccg
       61 cagggaaaga agcgcgtccg cgtcaaagtc ggcgacgggg agcttttggc aactgtggcc
      121 aatttggttg gcatcggtcg aggaacggaa gcgctgcagc ctgctccgcg aacgacccgc
      181 acctctctag gaggagaacg ctcataccct gtcgaaactc cggtcgtcga tgtccgcggg
      241 caggcggtcg atgaggccgt cgatcaggta ttggccgcac tggaccgcgc cactctggaa
      301 gaagcgcact cgctacggat tattcatggc cacggcactg gcaagctcaa atccaccctt
      361 cggacgtatc tacaaagctc accatatgtg gcacacctcc gtcctggcga gcgccatgaa
      421 ggcggtgacg gagtcacgat cgtctccatt cgctaggctc aggcggaggg caaaagagac
      481 ggaccagttg gctaggaaat atggtacttg gccattcggt agcgaagggt gttgcgcgta
      541 atcttgagca accggctggc ttgggagacg ttccctgcgg ccttgcgcag tgcttcttcc
      601 aaaatgttct tttccatttc atgaaatgag aggccgaacg ctaacatcga tgttttttcc
      661 gcccccaccg ttggcgaagg tgcttttgcc gggcggaacg aggccggtaa gtgctccggg
      721 tgtatggtgt cactcttgca attgatcgtg agcccttcca cgatgttatg cagttcgcgc
      781 acgttaccgg gccagtcatg tcgttgcagg agggagagtg cttcaggcgc gatgccccga
      841 attcggcagc catgatgctt tcgcgcatga tcaagaaaag acgagaagat cggttcgatg
      901 tcgtcgggcc gttcatgtag cggagggatc cggagttgat acacatttaa gcgataaaac
      961 aggtcaagtc gaaagcgtcc cgccttaatt tgagcgggga ggtcctcatt ggtggccgcg
     1021 atgactcgaa tgtttacctt gatgctgttg gtatcgccga gtggatcgat gacatgatct
     1081 tccaatacgc gcagaagctt ggcttgcgct gagaggctca tctcaccgat ttcgtccaga
     1141 aagagcgttc cgccggccgc catctggaac ttgccgggct tggcctgctt ggcatcggta
     1201 aatgcgccgc gctgatagcc gaacaactcg gattccagca acggctcggg aataccggca
     1261 cagttgatgg ctacaaaggg acctttcgat cgcaggctgg tcgcatggat cgcacgcgcg
     1321 aacagttctt tgcccgttcc gctcgctccg gttatcagga ccgtggcgtc ggtggcagcg
     1381 acttcggcag ccaatcgttt gacaagttgc atctgcggcg acacgctgat gatatgctca
     1441 aatcccagca ggtcttccag agtgtcttct gaggggcgga tcgctcgagc attgagaatg
     1501 gacgtgctga cggcgtcggc agagagtggc gtaggaagat agtcggaggc tccttcgcgg
     1561 atcgcctcga tggcggcttt cgcggtatcc tgacggccaa ggacgacgat aggaacggca
     1621 ccgggtatct gtctggcttg tgctagcacg acagcgcggg aaatgcgtcc cgagctgtcg
     1681 tcgcatatga ccagcgtaat gtcgtgtcgc gtggcgagaa gtcggacacc ggcttcggtc
     1741 gtcgtcgccg tcagaagggt gccgccgcaa tcggataacg cctgttcgag aaccgttctc
     1801 gatgtgggat ctgcagaaac cagtgcaacg atcggggtgt gcataacaac gctccaccgc
     1861 gagctgccga acaaccaggt tagcctactc ctatttctgg gctgatggaa ggggcaaggc
     1921 ttcgttgtag actaagagtt tacgtctaag tactgctttt gtcgttctcc tgcgcgattg
     1981 cgacattacg ttgcaaaccg gacagcttcg accttcgaat cggactatgg cggaatagtc
     2041 ccgcgaatgt ctcttcgtcg agggcagcta agtcatccaa gcggggaccc aacgttgtgg
     2101 cgtcgggctg aaacgccggc tccttcgtgg gttctgccct gagattgaac ggacagacgt
     2161 caagacagtc gtcgcatccg aagatgtggt ttcccatacg ggccgtcaga tccaccggaa
     2221 tatcgcggcg agggcctcga aactctatcg tcaaatacga aatgcagcgt gttgcatcga
     2281 caacatacgg ttccacaatg gcattggtcg ggcacgcctg gatgcacaac gagcacgtcc
     2341 cgcacagatc cgtcccggga gcatccggtg gcaactccag ggtggtcagg atctccccaa
     2401 gcaccagcca cgagccatgg tcggaagaaa cgaggttgga gtgtttgccg atccacccaa
     2461 gaccggcctg ctgcgcccaa gccttctcca tcaccggccc cgtatccacg tagggccgtg
     2521 tgatggcctc gggcaccaaa tcgcgcactt tgttttccag gagcaccaga cgattgctca
     2581 atacgtcatg gtagtccttc ccccacgcat agcgggcaat acggccatgt cctcgtccct
     2641 cgatggcacg aagtccagtg tcgtagttca tccccaccga gatgattgag cggcatccag
     2701 gcaaaacgat ttgaggatgt gcacgtcggt caggatcgcg cgccatccag gccatggtgc
     2761 cgtgatagcc tcgccgcagc cattcttgaa ggcgatccag caaaccgtga gccgggggtt
     2821 ccaggaccac tgaggtaatc ccgacggcgt caaaccccag taatcgagct tcgcgcttta
     2881 ttgcttggcc tatggacatg gcgagcaaga agactcccgg acacagtcac cacgtgcccg
     2941 aaagtggcag tcaaacaacg tgtcccgtca tcgtatctag cgggcggagc gcagggaagc
     3001 gtttgtgatc cttgctagtg ggggttgcag tcgaaccgaa cgatgaattg gacggaacag
     3061 tgaccggtgc tgcaacgacc gcaagtaccc gtgatcttgg tcttccaatc cgtctgcttt
     3121 tcgtcgaagc ggcagaaggt ctttccccct ttggaaatcg tcgtccaagg cttgttggta
     3181 ccgggtgcgt tcgccaccag acagccgtga ggaattggga cctggcaatt gtgcgagggc
     3241 tcgcctttgg ccatcccaaa actgcacgcc tgttcggtgg tggcgggaaa atccgaaggc
     3301 acctggcctt gggcatctgc cgggcgtaac aggaggaagg cacaaagcat gaggccgccg
     3361 aggcttagga gagatggcgt cacgcgcatg cagggatcgt atcacagtct ctttgcttgc
     3421 ggccaccgag tcgactgcgt agaatctgtc aggtcaaggg tggtgttcgg gccatccgga
     3481 cctcgggcca gtcattaggg gagggtacca tgcttgcgac gcggattcgt caaaaggaag
     3541 gcacctttta cttcatcgct tacaatgcgg ccgatctcct ggacaaggtc cgcttcacca
     3601 gccggtactt tttcgaaggt gaggaaatcg cgcaagggaa aatctccgaa catgatgatg
     3661 tggcgcagtt cattgccggc attgagcgaa gcgaaaaagg ctttcaacgt caactgaacc
     3721 ggcaaaagat caagcagatt gtcaattttt acgagaccgt cgtcgctcaa cccatgattc
     3781 ccgggacggt tttgatcttc acggacgaga cgctgaggtt ccaacgtgca ggggagtcgg
     3841 agtcgatcgg gcatttgagc gagccgaaag gcaagtacct gatcatcgac ggccaacatc
     3901 ggctggcagg actccatttt tttcgcgcga agcatccgga ccaaatcgcc aatgtcgagg
     3961 tgccgtgcgt cttgtttgac ggccgtagcg gagactttgc gaccgaaatg ttcgtcgtca
     4021 ttaacagcac ccatacccgc atcaatcgtt cccacttagt cgacctgtat gaaaaggtct
     4081 cgtgggaaac gccggagaag aagtttgcgg ccaaagtcgt caacctgctc tacgcggagg
     4141 atgacagccc tctccaatat aagatcaacc ggttgggcgg gaggagtaag caggaaaaat
     4201 ggatcctgca gtcagaagtc ttcaatgaac tgctcaaggt agtgatcgct cacaagcgct
     4261 ggatggaatc gcatctcggt atgaaggcgg agcgttgtta cgcgctggtc cgcgactatt
     4321 tgaaaggcgt gaaagaggtg atggaggaag tgtggggtaa caatgatcgg tacatgttta
     4381 cccgggacgt cagcctcaaa gcgttgattc gagtccttga cgacctgatc gtggatcgca
     4441 agctgatcaa tgattgggac gcccagcggt cacataagcc gtttgcagag attgtcaaac
     4501 cgtgggccgc attgtccaaa gaatttcgtg ccgaaggatt ttacgaacga tttcccgcca
     4561 aagggcaact cgaacgggtc cgaaaaattc atcagcggct gcttgatgcg atcgtcgggt
     4621 agcgtcgtcc cctccgtatg ttggttgctc atcctctgcg tctgctgtct gtgggcaagc
     4681 cttcccgcct ccgctgacgc tccccaggaa catctcgacg tgaagacgga tcctgccggc
     4741 ggcgtcatcg ccacggcccg gcttgtgttt ccggccacgc cgcatatcat tcaatcgctg
     4801 ctcaccgact atgcccattg gcccgatctc tttgaagtcc ggatgcggat cgtcgatctg
     4861 cagatcgccc gcggcgtcgt gaccaccgat ctgcgcattg accacgcttt gttgcccgga
     4921 gagcgccgat tggtcacgga atcccgcgtg atatcgggag tcatccatac ggatcttgtc
     4981 gcgggagact tcaagcgcta tcaccgcgta tggagattgg cgcccacgaa cgaaggacgg
     5041 gaaacgcgcg ccgaattcga gctgagtgtc gccgtcgatt ccatccttcc cgattggctt
     5101 gtggccttgg caatgaggcg ggagcttgaa gcgcatttcc ggatcgtgaa ggaaaaggcg
     5161 cgggcactgg ccgctagctc ggaaaagtga actccgagga ttcgacccga ttcatgccct
     5221 gtttgacgag cgtaccgata tcgagactgc gctcaattcg gcgaatgtcg tccatccggg
     5281 ttttggtgct ggggtgcgaa ggcgtaaaga gggagcaaca gtcctgatct ggctcgatcg
     5341 aagtcgtgaa cgtgccgatt cgctgagcct ggtcagtgat ctcgagcttg tccatcccga
     5401 tcagcggacg aaggacagga aactccgccg cctcttcaat tactgacaaa ttttccggtg
     5461 tctgtgaggc cacttggccg agactatcgc cggtcaccag agcccagcat ccttcccgtt
     5521 gcgccaactc cgaggctatc cggaccatca gtcgacgata gagcacaatc cggtacggtg
     5581 cgggagcctg ggctacgatt tcccgctgga tttcgccaaa gggcacgatg aagagcttgg
     5641 aatgacactg atacgcagtc aaggtttgaa cgagatcgct gaccttatcc tccgagtccc
     5701 ggctcaccag cggacgtccc gaaaaatgga caaagactgc gttgcaaccc cgtttcatca
     5761 tgcgataggc cgccaccggt gaatcgatcc ccccggacac cagacaggca accttcccgc
     5821 tcacgccgac aggcagtccg ccagggccgg ggaacttgtc cagataatag taggcatctt
     5881 tcgccagcat ctcgacgtag atgatcaagc ccgggtcggt gagcctgacc ttcttgcctg
     5941 tgaggtcgca aacatacttg cccacggctt tttcgacgtc catcgatgtc agcgggagac
     6001 gtttatccgc ccgcttggcg gtcacacgaa aggtgtcgaa ggcccgctgc ttgacggcgt
     6061 ttacgattcc gcgggtcagt tcgccgagat caggcttgag caggttgagt ggcacggaat
     6121 gcgcgaggga aaagttcgcg atgccaaaga tgagcgccaa gcggcttctc gcggcttgat
     6181 cgctgatgtg ttcagggagg acgatgcgga tccggctgcg cagactctcg atgcgtgttg
     6241 cgccaagatc cttcaacacg ctgcggatgt tatggatgag gcgctgctcg aaaaaatccc
     6301 ggtttcggcc tttgagagca agttcgtgat aatgaacgat gacgcagcgc atctgtgggc
     6361 atcgggcctt cgcttcagtg atgtcctttt agttccaaaa acttctccgc gtcgatcgcc
     6421 gccatgcagc ctgttccggc cgcggtgatc gcttgccggt atatcgaatc ctgcacatcg
     6481 cctgcggcga acacgcccgg cacactggtt gccgtgccgt gcttcgtcac aatgtagccc
     6541 ttggcgtcca tgtccaattg tccggcaaac aaggccgtat tcgggcgatg cccgatggcc
     6601 acgaacaccc cggaggcggg caaagtcgtc gtcgttccca tcaccaagtt gttgagccgc
     6661 acgccgctga ccgtttcctc accgagaata tcttcgacga ccgtattcca aatgaaagag
     6721 atcctgtcgt tcttaaaggc acgatcctgc ataattttgg aagcccggag tttatcccgc
     6781 cgatgcacga cgtaaacttt agtggcaaac tttgttagaa aattcgcctc ttccatcgcg
     6841 ctgtctccac ctcctacgac caccaactcc ttcccacgga agaaaaatcc gtcgcatgtg
     6901 gcgcaggtgg acacgccgtg cccggtgagc ctggcttcat tcttgagtcc gatctggatg
     6961 gcggaggccc cggtcgcaat gatcacggtt cgagcttcga tcgttcgttc atggtccacc
     7021 gtgattttta agggacgggt accaaattct gcggcgctga cgtcaccggt cagaaagtcc
     7081 gtcccgaacc gttcagcttg tgcccgcatt tctttcatca ggtccggacc catgatccct
     7141 ttcgcgaagc cgggaaaatt ttccacttcg gtcgtggtag tcaactgccc gccggattgc
     7201 cagccttcaa ccagcagcgg agcgagattg gctcgagccg cgtagatggc cgcggtcagg
     7261 ccagccggac cagagccgac aatgaggacg ttgcgcatac agtcaatcta gcacagcaaa
     7321 ccacggatta cacagggcgg tcgcctgcac ggaaatctcg aaggagccgg cggatctccc
     7381 tgctcaggag agacttgctc tgggtgccat ccaacacgat gtcggctcga cgtgccttct
     7441 ccgcaagcgc catctggcta ctgatacgct gaaacgcctg ccgcctcgtg aggccgttgc
     7501 gccgcttcaa gcgggcgact tgcgtttcac ggtctgcagt gaccacgatg attttatcca
     7561 cgcgcgtgtt gacaccggct tcaaacaaga gaggcacttc gtaaataatg attgcatcgg
     7621 gagtgcgtct ggcaatgcca tgcgcccggc gttgctgttc gcgtgctacc cgtggatgaa
     7681 tgattcgctc aaggcgccgg agctttcggc ggttgtggaa gacaatggaa ccgagagcat
     7741 gacgatcaat cgtccgatct ggcagcagga ctcgcttgcc aaaaattttc acgatttcgc
     7801 gccaggccgg ttttccgggc tggaccacgt cacgggccaa ctgatcggca tcgatgacgg
     7861 tggcgccaaa gcgtctaaac atgctggcga ccgtgctctt tcccgttgcg attcctcccg
     7921 tcaatcccac gagaatcatg cggcgaagaa taccatgtgg gtgtcgagaa agtcgttgag
     7981 cagcctcgag catacgattg acttcggacc ggccccgcct gtatgaacgt gaagattatg
     8041 ctgatgcgaa caatcggctg tctgaccttg tggtctcttg ttgtgtgcgc tgtcgtgcac
     8101 gcagagagcg gctctgcgca ggccccacgg acgatcgtgg tcgctttgga tgggagcggt
     8161 gacttccgat cgatacagga ggccgtcgat gcggcggcaa agggcgacac ggtcttcctc
     8221 aagccggggc gctatgaaca ggacatcacg attcacagca aggatcacgt caaactcatt
     8281 ggagcggggc aagacaaagt caccttggtc ggccgtgaag atttggtggg agtccttcat
     8341 gtgggcaaat ggccgtacgg cgcgacaaac attgaaatca gcgacatgac gatcaacgag
     8401 catggcggtc atgcggtggg gctgttcaac ggccggggcc tattgctcaa gcgtctcaca
     8461 gtgaacggca tgctgttcac tcagcaggtc gaagacacgc gcattgaaga ctgcaccatc
     8521 ggaggtagtg agactacggg tgtgcagttt gcggactctc aagcagtctt ggtcggtaat
     8581 ttcattcatg acaatgacca cggggtgaac gtcgcgggca aatccaatgt tcgtctggaa
     8641 cgaaacgtca tcaccagaag cctgttcgag gccgtagtgg tcaatgatcg cgccaaggcc
     8701 gtgctggtca gcaatacgct ggtcaaaaac gggggcgggg cggcctttct tggccagtct
     8761 cagagcgatg tcagtggtaa tgtcgtcggc ctcaatcgag tcgggttcct cgtcgcgtcg
     8821 tccagtcgag cggcgacctc gtataacgcg ctatacaata gcgacggtga ttatctccga
     8881 gaaggaaatc ccaatcaaat tgcccccgag ctcaaggcgc aatcggacat cactggcgat
     8941 ccgtattttg tcgatcccgc aaagggtgat ttccgactcc gcgcagacac cccgttgcga
     9001 aaaattggtt ctttcccgtt tcttggcgcc cttcctcctg ctgagtaaac tacttagtat
     9061 tcaacggctt atgctgctgg cctggttgct gccatttcgt gtgctaagct atcagtatgc
     9121 caggtgtcgt aaggactggc gtgacaattc gaaacgggtc ataggtagga ggcaaagcgt
     9181 ggctagctac aaacaagagc ttgagaagcg cgggcccgaa ggtatcccat ctctcgatga
     9241 cattgaaccg gggaaaatct gcggggccgt cattcccatg tctgaacgcg cacagactca
     9301 gatgcgggat agcatcgaag tcatcgatta tctcttgaat caggaacgtg ttgtctggag
     9361 ttaagcaccg catctttccc ctcgtcacgg taagctcccc ttgacacttc ttccctcttc
     9421 gtagtcttct tgccctgtcc gtccacctgc tcaagaggag atgcctgcat gaaatttctg
     9481 aaccgtcttc gtctattcca ctcatacgcg agtgtgtttt tgattgcgac cggtcttgtc
     9541 atactcgtct gtccctcgtt ctcatttgca gatgaattac cgaaagaagc cgtattgccg
     9601 ctggcaatgg cgagcaaggc ggctcaagct gccatggaag cctgcaagaa agacgggtat
     9661 cgcgtcagtg tttctgttgt ggaccgagct ggtgttttac ggactatggg acgggccgat
     9721 ggagccgggt cccatacggt cgaaagcagc aggaaaaagg cctatacggc tgcaagtctc
     9781 cgccgggcga cgacggaact ggcagaactg atcgggaagg ttccgacgct tcagtcgctg
     9841 agggagatga atgacgaaat cctcatactt ggcggcggtc tacctattga aatcggcggc
     9901 gacgtcgtgg gcggcatcgg ggtgggcggt gcgccggggg cacatctgga cgatgcctgt
     9961 gcccaagcgg gactcgatgc tgtcggggcg gctcccaaag ttccagcggt caagtgatcg
    10021 gcagctgggg taggggcggc ctgcttggct gtatcgggct cctcaatgga tgtaacagcg
    10081 acgtgcctgt ttctgcggtt actcaggtga cgcctcccgg attcatcgtc agcgtcaccc
    10141 gcatcgccac gcatccattc ttgtcccgtt tcaatctcgc actcacggtg cgtacggctg
    10201 gtggctgtgc agcgaccagc gagctgtttc cggataccgg cggtgtaagc cggcgcaatc
    10261 tctatctgac tacatcggac cggctgtacg tggttgggca gttcgatgtg cgccgattcg
    10321 acccgcagag atgtctcatc gaactagtcg aattccgctc ggtggaggac ggcctgaggt
    10381 tcattggctc gtttgacacc gatcgttcag gccaatgggc gtttcagaca gccagcgcgc
    10441 aggcggaacg tccgttcgag ccgctgtgac aatcccttca aggcgatgcc atgtaccact
    10501 caaggttatg atggaccaac agatcgtggg ataccaccaa gatgaggtcg gggattgggt
    10561 tgcggacctc gcatgcggac acggacagca tgtgcgtcat cacccaccta tgacaaaccg
    10621 gccttgggtg ttgactcaat ccggtcgaga ccagcatctg ggcaccatat tgaactgtca
    10681 gaagtgtgaa gagactcaat attgaagcat ttctattcga tctgctttcc ctggaccggc
    10741 acctactcgt ccgacttgat cagatcgtgc acggatgggc tcaccgcctt gcggtggccg
    10801 gaggtatacg gtccaatatg gaaacgggtc agctccggct attggcaggg aataaccgtc
    10861 atcagcgtgt gagagtggct attgcgtcgg gaggacgaac aagcaggtct aggccaggcc
    10921 ggtgccaggg cacgaggcga ggcccgcggt attgataccc gtctatgacg gggtcgttgt
    10981 ccaaaagcag cggggtatcc agatcgagga cctcgaagcc ttttacaccg agcaccaggc
    11041 tgaatgagca gcccatcgca attctcgttt cgaccatgcc gccgaccatg agtttgaggc
    11101 cggagccgag ggcaaacagg gcgatttcat gagcctctag gacacccgtt ttcatgatct
    11161 tgatgttgat gtaatccgcc gcgtgccggt caaccacctg ctttgcgtcc acgagagacc
    11221 ggacggattc atcggccgca acgggaatac ccgtctctcg ccgaatcgca gcaagcgact
    11281 ctaagtcctc tcgtaggacc ggctgttcga gaaggaccag cttccccccg aaccgtgtca
    11341 ctccttttgc gaatgcgaga cattcccgat gcgagaatcc ct
//
LOCUS       DASTQY010000156         5842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_41706, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000156 DASTQY010000000
VERSION     DASTQY010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41706"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="VFR82_05095"
     CDS             complement(<1..1196)
                     /locus_tag="VFR82_05095"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU4501008.1"
                     /translation="MNGATHKVFTILLVEDNGGDVEMFLRTVEEELPREDDEDVELVI
                     AARAEGALAILDERRIDLVITDVRLPGLSGIELLKRIQARDRRIPVIIISWVNTVDTA
                     VEAMRCGAFDYVTKPFEKLDFAARIHRAMRISEILFRYEPPQIGEAIPFKELVGVSPA
                     FKNVTAMIDAAARVQSTTLIVGETGTGKELIARAIHERSAERNGPFQVVDCTTFSEGT
                     VESELFGHVRGAFTGAVGDKLGLIERGMGGTLFLDEIGDLPLPLQAKLLRVLEEGEMR
                     AVGSVQMKKVSARFIAATNQELTEKVKRNEFRKDLFFRLNVMAIKVPPLRERTEDIPL
                     LARHFITRYTKEFGKYVTDVHPLAVTELVAYPWPGNVRELRNVMERAVMLAKGDRIHP
                     QDVIGLL"
     gene            complement(1193..3268)
                     /locus_tag="VFR82_05100"
     CDS             complement(1193..3268)
                     /locus_tag="VFR82_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4501009.1"
                     /translation="MAAIQSTSCGWLSGWQHSILLKIGALFVLLSLAGSTYLVTASNL
                     TGQLTGLTKAIEQAGTERMRIYKLASLLERFPPSEQNRVAIRVEMSHFERVVEGLRFG
                     TLQHGPVASNNPILSAQLQALQDRWSTRLKPALEVALGGDGASIAAQDYLQQADDFVA
                     GWDQFVKLMEQETAARLQTLRQRQIWSLVAFLALITAAIVFLNRFVREPLRHLTAGAN
                     RLAAGAFDAEIQVHSNDELGQLARTFQHMAETIRHHIGELNNLHATGQEIAMLGPGGL
                     EDVLRRIADRAAESLEADLAVVMVRHPTMECWLVEAASGQAFDRIRKQILLLEETPFS
                     NQALDTKEPVVVADVSAYKDGPMRFRDEFHAKSFLGVPLLGPHECLGVLVLLSTVRMR
                     TFTEWDIRMAQQFASHAAVAMENAHLFDALESESQVLQKQLCAVERQVAELTHEVKAP
                     AGRVAEFASWIERDYGRLLDAKGLQYLAWIKNEGKDLATLAERTLDLARINHQPAPLE
                     SVDVGSVVREILSFLEQDGHTEGIRVTIASDLPKLLCRRIHVKQIFENLLGNAIKYMG
                     RQADPHVHIGWLDDERGTKVFVRDNGVGIDATMLDRIFLPFVRLRTEGVAGSGIGLSI
                     VKTVVEQYKGSVTVDSLPGAGSTFYVQLPVPPRTSYLKPPPDTRAGREGEQPADRQCM
                     PEVAKELFT"
     gene            3951..4352
                     /locus_tag="VFR82_05105"
     CDS             3951..4352
                     /locus_tag="VFR82_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4501010.1"
                     /translation="MHKCRVLLVDDHVPIRIALRRVLTSYDNLHVVGEACDGKQAIEL
                     VPVYQPHVVLMDFYMPRMNGIEAARIMKESWQEIAIIGISTAPDTFITDAFVKAGALA
                     VVSKDQVDHLYSTIQQVFSHNIRGNIGPLHQ"
     gene            complement(4462..4590)
                     /locus_tag="VFR82_05110"
     CDS             complement(4462..4590)
                     /locus_tag="VFR82_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501011.1"
                     /translation="MNKLKAAAALSGGQSIQQYVVQLLLDHVAELERKGQLPKGPR"
     gene            4774..4992
                     /locus_tag="VFR82_05115"
     CDS             4774..4992
                     /locus_tag="VFR82_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501012.1"
                     /translation="MAKSTKPDFLREARLQDCLVFLDKLRKSGVTNMFDIRLTLRAAF
                     PDLTTKQAAEVVAYWMRTFTARAHREAS"
BASE COUNT         1304 a         1651 c         1584 g         1303 t
ORIGIN      
        1 agcagtccta tgacgtcctg ggggtggatc cggtctcctt tggccaacat gacggcccgc
       61 tccatgacgt tacgcaactc ccgcacattg ccgggccagg gataggccac caattcagtc
      121 accgccaaag gatgaacatc ggtgacgtac ttgccgaact ccttcgtata gcgtgtgatg
      181 aaatggcgcg cgagcagtgg aatgtcctcg gtccgctccc ggagcggggg aactttgatg
      241 gccatgacgt tgagacgaaa gaagagatct tttcgaaatt cattccgttt gaccttttcc
      301 gtcagctcct gattagtcgc ggcaataaaa cgcgcactga cctttttcat ctgtacactt
      361 ccgaccgccc gcatttctcc ttcctccagc acacggagca gtttggcctg cagcggaagg
      421 gggaggtcgc cgatctcatc gagaaacagc gtacctccca tgccccgctc aatgaggccg
      481 agcttgtctc ccacggcgcc ggtgaaggcg ccacgcacat ggccgaacaa ctcgctctcg
      541 actgtgcctt ccgaaaatgt cgtacagtcg acgacctgga acggaccgtt ccgctcggcg
      601 ctccgttcat ggatcgccct cgcgatgagt tcctttcccg ttccggtttc tccgacgatc
      661 aaggtcgtgg actggacccg ggccgccgcg tcgatcatcg cggtcacatt tttgaacgcg
      721 ggactcacgc ccaccagttc cttgaacgga atggcttctc cgatctgcgg cggttcataa
      781 cggaacagga tttctgaaat ccgcatggca cgatgaatgc gtgcggcgaa atcgagtttc
      841 tcgaacggtt tggtcacata gtcgaacgca ccgcaccgca tggcttcgac cgctgtgtcg
      901 accgtgttca cccaactgat gatgatgacc gggatccgcc gatccctggc ttgtatgcgt
      961 ttgagcagct cgatgccgct cagaccgggc aggcgcacgt ccgtgatcac cagatcgatg
     1021 cggcgttcgt cgagaatggc aagcgcgcct tccgcgcgag ccgcgatgac caactcgacg
     1081 tcctcatcgt cttcacgcgg caattcttcc tcgaccgtcc ggaggaacat ctccacatcg
     1141 ccgccgttgt cttcgaccag gagaatcgta aagactttgt gcgttgcacc gttcatgtga
     1201 aaagctcctt tgctacttcg ggcatgcact gtcggtctgc cggttgctcc ccttctctcc
     1261 cagctcgagt gtccggtggc ggcttcagat aggaagtgcg gggtggaacc ggaagctgca
     1321 cgtagaatgt gctccccgcc ccaggtaatg aatccaccgt gacagatcct ttgtattgct
     1381 ctaccaccgt cttgacgatg gaaagaccga tgcccgatcc cgccacaccc tccgtccgta
     1441 gccgtacgaa gggaagaaag atccggtcca acatggtggc atcgattccg acgccgttgt
     1501 cccgtacaaa gactttcgtg ccgcgctcat cgtccagcca tccgatgtgg acatgagggt
     1561 cggcttgtcg tcccatgtac ttgatggcat tgccgagcag attctcgaag atctgcttga
     1621 catgaatccg tcgacagagc agtttgggca gatcgctcgc aatcgtcact cgaattcctt
     1681 cggtgtggcc gtcttgctcc aggaaggaga ggatctcgcg cacgacggag cccacgtcca
     1741 cgctttccag cggagccggc tgatgattaa tgcgcgccaa atccaacgtg cgctccgcca
     1801 gcgtcgcaag gtctttccct tcgttcttga tccaggcgag gtattgcaac cctttggcat
     1861 ccaaaagccg gccgtaatca cgctcgatcc aagacgcgaa ttcggctacg cggccggctg
     1921 gcgctttgac ctcgtgggtg agctcagcga cctgccgctc aaccgcgcac aattgctttt
     1981 ggaggacttg agattcggat tcgagcgcgt caaacagatg cgcattctcc atcgcgactg
     2041 cagcgtgaga ggcaaattgc tgggccatcc ggatatccca ttctgtgaat gttctcatcc
     2101 ggacggtgct caacagcacc aagaccccga ggcattcatg aggacccagc aacggcacgc
     2161 cgagaaaact cttcgcgtga aactcatccc gaaaccgcat cggaccgtcc ttgtaggccg
     2221 acacgtccgc gacgacgaca ggctccttgg tatccagcgc ctgatttgaa aacggcgttt
     2281 cttccagaag cagtatctgt ttgcgaatcc tgtcgaacgc ctgccctgac gccgcctcca
     2341 ccagccagca ttccattgtc ggatgacgca ccatgacgac ggctaggtcc gcttccaatg
     2401 actccgcggc ccgatcagcg atccggcgca ggacgtcctc caagcctcca ggccccagca
     2461 tggcaatctc ctgtcccgtc gcgtggaggt tgttgagttc gccgatgtga tgtcggatgg
     2521 tttccgccat gtgctgaaac gtccgggcta actggcccag ctcgtcgttc gagtgcactt
     2581 ggatttcggc atcgaatgcg cccgcagcca ggcggttcgc gcctgcggtc agatgcctga
     2641 gcggttcccg cacgaaccgg ttcagaaaca caattgccgc agtaatcagt gccagaaagg
     2701 cgaccaagga ccagatttgc cgttgccgca aggtctgcaa ccgcgcggca gtctcctgtt
     2761 ccatgagctt gacgaactgg tcccatccgg cgacaaaatc atcggcctgt tgcaggtagt
     2821 cctgtgcggc aatcgaagcg ccatcgcccc ccagggctac ctcaagggca ggcttcagcc
     2881 gcgtcgacca gcgatcttga agcgcttgga gttgggccga gagaatagga ttgttcgatg
     2941 caaccggacc gtgctgcaga gtcccgaaac gcaggccttc taccacgcgt tcgaagtggc
     3001 tcatctcgac tcgaattgcc acgcggttct gttcggaagg cggaaatcgt tcaaggaggg
     3061 aggcgagctt gtagatccgc atgcgctcgg tgccagcctg ctcgatcgct ttagtgaggc
     3121 cggtcagttg cccggtgaga ttggacgcgg tgacgaggta cgtggagccc gccaaggaca
     3181 gtagaacaaa taacgctcca atctttagga gaatggagtg ctgccagcct gatagccagc
     3241 cacaagatgt ggattgtatc gccgccatat gcagcgactc caaaaaaccc tagggtattt
     3301 aggtcaacgg atacatccca ccgatcgaaa gccttctcct gagcgaaggt ccgaatacta
     3361 ccgctatttc ggccgaaaat gccagtagga cctatccact gattcgctag gctgggccac
     3421 ctataaaacg gctagtaagc agtattgatg agctctccag tgctatttca tcagcacgat
     3481 aaccacgatg gtaatattgg tttgacctac ggtttgtgag ttcgcttttc ttccagtacc
     3541 gaccatatat cctttatcta cacgagccgg tgaattgagc ctgccattaa agactgttaa
     3601 agttccactc cagagcggcc acactcttcc gaagtcggac gaaaaaacct ttttacgtgg
     3661 gcggggtcta ttgcggggtg aggtgcgata agtacgcctc caaccactct tgcctcaggg
     3721 atagccgtct ggccgactat cagtcagtca gaggagggtg gggccatttt ttggaaaagg
     3781 ggtgtcacct acatggccgc tgccagttag gtaaagagga atacagaagg ttcgccggtc
     3841 accgcttgcc aacccttgta gagagcaaaa tcctagctcg accctgggaa tgttactaat
     3901 gggacgcgac atatgcttgc tgtattacag atgagtaaga ggctagcgtc atgcataaat
     3961 gtcgcgttct gcttgtcgat gatcatgtgc caatccgcat cgctctacgt cgcgtgctca
     4021 ccagttatga caaccttcat gtcgtgggag aggcctgcga tggcaagcag gcaatagaac
     4081 tagttcctgt ctatcagcct catgtcgttc ttatggactt ctacatgccg aggatgaacg
     4141 gcattgaggc tgcgcgcatc atgaaggaat cctggcaaga gatagcaatt atcggaattt
     4201 ctacggcacc ggatacgttc ataaccgatg ccttcgtgaa ggccggcgct ttagcagtgg
     4261 tgtcgaaaga ccaagttgat catctttatt ccacaattca acaagttttt tcccacaaca
     4321 ttaggggaaa cataggccct ctccatcagt gaaaacggtc gacgagccag aagtgttgcc
     4381 tccctggcca atcttataca ctcagcgata cctgatgcca caggactgag tttagacttg
     4441 tgtacgcgaa acaggagtgc cttatcgggg gcctttcggt agctggcctt tgcgttcaag
     4501 ctcagcgacg tggtcaagca gcagttggac gacgtactgt tggatgcttt gccccccgct
     4561 taaggcggcc gcagccttca gtttgttcac gagatcattt gggacatttc gtaaatagag
     4621 caccctagga gtagccatcg ggggagcgat gataccgcac taactccggg caacacaagc
     4681 tcactttact tgcggactag caaagattag gatgtgctag caactgacgc attgctgtct
     4741 tatatgacga agcaagcaaa agcggatgat cgcatggcta agagcaccaa gcctgacttt
     4801 ctaagagagg cacgactcca agactgcctg gtgtttttgg ataaactgcg caagagcggc
     4861 gtgacaaaca tgttcgacat acggctaaca ctacgcgctg ctttccccga tctcacgacg
     4921 aagcaagctg ctgaagtggt ggcttactgg atgcgcacct ttactgctcg tgcacaccga
     4981 gaggcctcat gaagatcctc attacgacag ctgattgcgt caccataacg ttcgcgacat
     5041 ggcaaccatt ggtgcccggc caccttcgcg acagggtgcg ttttactgag cctagttgaa
     5101 cgaccgcgga gatatggtaa tcgtcacacc ttcgtgtccg tccaccttca agttcagttg
     5161 gccgttgcta ctcagatacc gcagtgcgtt aaagacctct cttagagata aatctggaag
     5221 gaggtgggcc acatgtctca tctggcaccc tggtgcattg gctattattt caaagatgcg
     5281 atcagtaatg ctctgaccca atgtctgcat gatgatctcc tgacagttta gtgggcagaa
     5341 gcggtgcggc ctttcgggcg aatgttatac aataggcttg cgctagggtt ttggatagat
     5401 gggactcata cttggggcct tttactatgc tccctggaat aatgagaggg gatttccata
     5461 gtgggggatg cggcgatgac ggcaagggag tgttaacgct tggcaaagag tcgtgctagt
     5521 ggcgataaag ggacgagtgg cgggaggcca ccattgcgat agggaggtgg ctgcatagcg
     5581 gactgccagt cggccaaaga cacatacaaa agcagaataa aacgacaggc gaggggcgct
     5641 agttgagaat aatagttgcc cactagccat attgaactcg aagccaaccg aaaggcatcc
     5701 acacgttgct cacctgccac ttatgccacc cactgaagca actcccccag tcgttctcgc
     5761 tcgcttgagg gtatagacag gaactggaga ccaaagtcct tttcacccac ccattttacc
     5821 gccgctcgtt ccacaacaat gg
//
LOCUS       DASTQY010000157         4208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1604855, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000157 DASTQY010000000
VERSION     DASTQY010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604855"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..680)
                     /locus_tag="VFR82_05120"
     CDS             complement(81..680)
                     /locus_tag="VFR82_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HEU4501013.1"
                     /translation="MRFSVSILVALFVLSLASPLMAKDKKSGKPMDQQAMMELWKKMA
                     MPGEPHKLFATLAGSWTTTSKEWMEPGKPPTESTGTAEMKMLLDGRFLYQEYNAQMMG
                     QPFTGIGIDAYDNMTKKYVTAWMDSMGTGIFIMEGTASADGKTITLKGSHPEPDGGKM
                     THRAVWKIVDNNHQTFDMYGAHKGGKETKMMEIVYTRQP"
     gene            complement(728..982)
                     /locus_tag="VFR82_05125"
     CDS             complement(728..982)
                     /locus_tag="VFR82_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501014.1"
                     /translation="MLSFIEMAVVILIWGDACRCRDASRFVCIERSATIKASTERLFR
                     SCPQPYMANLTSLFLSMKKVVGRQFEQGLANLKTVSEQEG"
     gene            complement(990..1415)
                     /locus_tag="VFR82_05130"
     CDS             complement(990..1415)
                     /locus_tag="VFR82_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU4501015.1"
                     /translation="MRFMIIVKATKESEAGVMPNEKLLSEMGKFNEELAKAGILLAGE
                     GLQASSKGARVKFSGSTRTVSDGPFAETKELIAGFWLWKVNSKEEAIEWVKRCPNPHN
                     EDSEIEIRQVFEAEDFGADFTPELREQENRVRAQAAKAR"
     gene            complement(1576..1926)
                     /locus_tag="VFR82_05135"
                     /pseudo
     CDS             complement(1576..1926)
                     /locus_tag="VFR82_05135"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1898..2263)
                     /locus_tag="VFR82_05140"
     CDS             complement(1898..2263)
                     /locus_tag="VFR82_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU4501016.1"
                     /translation="MKYMLLVFHNERSFTELGEATHANMLDESIRLAHELASKGQYLA
                     APLQPTSTATCVRVRDHKHMVTDGPYAETHEQLAGYFLIDVKNLDEAITVAARVPGAR
                     IGTVEIRPVRDVPGLPTVR"
     gene            complement(2389..3639)
                     /locus_tag="VFR82_05145"
     CDS             complement(2389..3639)
                     /locus_tag="VFR82_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU4501017.1"
                     /translation="MSEEARHNAREAINEVYGAESRRVLATLIRLLGDFDLAEEALHE
                     AFAAAMEQWPRDGLPANPRAWLVSTGRFKAIDAMRRRARYDATLSDYAREHESLVTEN
                     AEPDDQDIDDDRLRLIFTCCHPALSPEAQIPLTLREVCGLTTEEIAHAFLTKPATIAQ
                     RIVRAKAKIRDARIPYEVPAPSQLPERLDAVLRVIYLVFNEGYSASSGEAVTRAEVSG
                     EAIRLGRLLVELLPDAETRGLLGLMLLQESRRAARTSADGDLILLEHQDRSLWQRDLI
                     TEGLALVTRALSTQRIGPYSIQAAIAAVHAEAAEADKTDWAQIVGLYDVLTRIEPSPI
                     VELNRAVAVAMRDGPLAGLALIENILNRGELTEYHLAHAARADMCRRLGRQAEARTFY
                     KRALALTKQGPERRFLEQRLSSLD"
     gene            complement(3682..3978)
                     /locus_tag="VFR82_05150"
     CDS             complement(3682..3978)
                     /locus_tag="VFR82_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU4501018.1"
                     /translation="MIRVVLPAHLRTLARVAGEVTVEVHGPVTQRSVLDAIEASYPML
                     SGTIRDHVTKRRRAFVRFFACEQDLSHESPDALLPDAVATGEEPFLIVGAMAGG"
BASE COUNT          832 a         1222 c         1192 g          962 t
ORIGIN      
        1 ctcgggattc cgtgacaggg ctccttgcca acttttgcct ggctcatgtg ctcttgttgc
       61 atatccaaca tccgtctagg ctatggctgt cgggtgtaca cgatttccat catcttcgtt
      121 tccttgccgc ccttgtgggc accgtacatg tcgaacgtct ggtgattgtt gtcgacgatc
      181 ttccagactg cacgatgcgt catctttcct ccgtccggtt ccgggtgcga gcctttcagc
      241 gtgatggtct tcccgtccgc gcttgccgtc ccttccatga taaaaatccc cgtccccatc
      301 gaatccatcc aggccgttac gtatttcttg gtcatattgt cgtacgcgtc gatgccgatg
      361 cctgtgaagg gttggcccat catctgggca ttgtattcct ggtagagaaa gcgtccgtcg
      421 agcaacatct tcatctcggc ggtgccggtg gattccgtcg gcggcttgcc cggctccatc
      481 cattccttgc tcgtggtggt ccaactgccc gccaaggttg cgaatagctt atgtggttcg
      541 cctggcatgg ccattttctt ccagagttcc atcatggcct gctggtccat cggctttcct
      601 gatttcttgt ctttggccat gagcggagat gcgagggaaa gcacgaaaag agccacgaga
      661 atgctgaccg agaaacgcat aaagacctcc tggggttaga cgatggtttc caaattgagg
      721 agggcgatca gccctcttgc tcactcactg ttttcaggtt cgccaaccct tgttcgaact
      781 gtcgaccgac caccttcttc atgctgagaa agagactcgt caggttggcc atgtacggtt
      841 gcgggcatga acggaacaat cgttctgtcg acgccttgat cgtggccgac cgctcgatac
      901 agacgaatcg gcttgcatcg cggcagcgac aagcgtcacc ccaaatcaag atgacgacgg
      961 ccatttcgat aaaggagagc ataaagggct taccgtgcct tggcggcttg cgcccgaacg
     1021 cggttttcct gctctcggag ttccggcgta aagtctgcac cgaaatcttc cgcctcgaat
     1081 acttgacgaa tctcgatctc gctgtcttcg ttgtgcgggt tagggcagcg tttgacccat
     1141 tcgatcgctt cctccttgga gttgactttc cacagccaga accctgcgat caactccttg
     1201 gtctcggcaa acggcccgtc actgaccgtg cgcgtgctcc ccgaaaactt tactcgtgcc
     1261 cctttggagc tcgcctgaag cccttcaccg gcgagcaaga taccggcctt cgccagttcc
     1321 tcgttgaatt ttcccatctc ggacagcagc ttttcattgg gcatcacgcc ggcttccgac
     1381 tccttggtgg ctttgacgat gatcatgaaa cgcatagcgg aaactccttt ctcgtaagtt
     1441 ctaatccggc agccctggct tctcatgcta gtcgaatgaa gcagtcaaaa tcgacagagg
     1501 tcagtgaaac aaatctgcta cggaagcaca cctcgaaaaa gacctagacg tcaggatctc
     1561 cgccggccga cagggctacc cagtaatagt ccacttcatc tgtgtcgcgc agtcgacctg
     1621 aaacgaaacg gcctcattga aggtgaagat cggcccgcca ttgggggcga cgaattccag
     1681 cccatctatt tcaaaggtgg ccgtcttcac cgagtcttac ggccgtcggg aaacctgagc
     1741 tccagcgtca ccgtatcgcg tgatcgttcc catgtttgaa tgcttgaaga tcgagagata
     1801 gaagcgctcc gcctcttcgg cgttttcgat gaaccataaa cagggtgaaa tttttggcat
     1861 ccaatgctcc ttggctccgc agctatggac caggtgtcta gcggacagtt ggcaggccgg
     1921 gtacatctct gacgggccga atttccactg tgccgatgcg ggcgccaggc acgcgggccg
     1981 cgacagtgat cgcttcgtcc agattcttta cgtcgatgag aaaatacccc gccagttgct
     2041 catgcgtttc ggcatagggg ccgtctgtca ccatgtgctt atggtcacgg actctgacac
     2101 aggtggctgt tgatgtgggc tgtagcggcg cagctagata ttgccccttt gaggcgagct
     2161 catgtgcaag ccggatcgac tcgtccagca tattggcatg tgtcgcttcg cccagttctg
     2221 taaagctccg ttcgttgtgg aagacgagca gcatgtattt catgcgatcc cccccggtgg
     2281 ttcgtcagtt gacccgtgac ctactagata gtcggcgtga gcaggaaaaa tcgacagcgc
     2341 acgcaacccc ccggaaagcg gtgagtgttc cagaccatgc gacggtattc aatctaacga
     2401 gctgagccgc tgttccagga aacgtcgctc tggcccctgc ttggtgagcg cgagggctct
     2461 tttgtagaag gtccgggctt cggcttgtcg cccgagtcga cggcacatgt cagcgcgggc
     2521 agcgtgggca agatggtatt ccgtcagttc gcctcggttc aagatgtttt caatgagcgc
     2581 aaggccggca agggggccat cccgcatcgc caccgccacg gccctattca gttccacgat
     2641 tggagagggc tcgattcgtg tgagcacgtc atagagtcca acaatttggg cccaatcggt
     2701 tttatccgcc tcagccgctt cggcgtgcac ggcggcaatc gccgcttgga tcgaataggg
     2761 cccgatccgt tgcgtcgaaa gtgcccgtgt gaccaaggcc agtccttcgg taatcagatc
     2821 ccgctgccag agcgaacggt cctgatgctc caataaaatg aggtcaccat cggcggatgt
     2881 acgcgcggcc cgtcgtgact cttgtaacag catgagcccg agcaatcccc tcgtttccgc
     2941 gtccggcagc aactcgacaa ggagccgccc gaggcggatg gcttcacccg agacttccgc
     3001 gcgggtcacg gcttccccgg aggatgcgga atagccttcg ttaaagacga ggtagatcac
     3061 gcgtaggacg gcatcaagcc gttcgggtag ttgggatggc gccggtactt cgtagggtat
     3121 gcgagcatcg cgtattttgg cttttgccct gacgatccgt tgagcaatcg tggcgggctt
     3181 ggtaaggaag gcatgggcga tttcttccgt tgtgagaccg cagacttcac ggagcgtcag
     3241 gggaatttgt gcttccggag acagggcagg gtggcagcag gtaaagatca gccgtaagcg
     3301 gtcgtcgtcg atgtcctgat cgtccggttc agcgttctca gtgacaaggc tctcgtgctc
     3361 tctcgcatag tcgcttaacg tcgcatcgta gcgcgcgcgg cgtcgcatcg cgtcgatcgc
     3421 cttgaatcgg ccggtcgaca cgagccaggc ccgagggttg gcaggcaagc cgtcacgggg
     3481 ccattgctcc atcgcggcag cgaaggcttc atgcaaggcc tcctcggcca ggtcaaaatc
     3541 gccaaggagg cggatcaggg tggcgagtac acggcgcgac tccgcaccat acacttcgtt
     3601 gatcgcctca cgcgcattat gcctcgcttc ttcgctcatg tggactgggt ccatgtcgag
     3661 aggggatgct gtggatgacg actagccgcc ggccatagcg ccgacgatca aaaaaggctc
     3721 ttcgcctgtg gctaccgcat cgggtagtaa ggcatccggc gattcgtgag aaaggtcctg
     3781 ctcacacgcg aagaacctca caaaggcccg ccgccgtttt gtgacgtgat ctcggatggt
     3841 tccactcagc atcgggtagc ttgcctcaat ggcgtccaga actgagcgct gcgtcaccgg
     3901 tccatggact tccacggtga cctccccggc gacgcgcgcc aatgttcgta aatgtgccgg
     3961 taagacgact cgtatcatcg tgtgcactga cgagttattg cctatgcaaa aaaagcatca
     4021 ccggagtcta caagcttttt ccgcggctgc ccacacctgg ggatcaggtt acagtgtctg
     4081 aacttccact gaaaggaccg acggcagatc ccggacaaga ggctcccatg tgtcgccgcc
     4141 atcggcggat ccgtagacct gcccgccagt ggtgccgaag tacaaaccgc agggatcgag
     4201 tgaatcca
//
LOCUS       DASTQY010000158         6679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1099156, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000158 DASTQY010000000
VERSION     DASTQY010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099156"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..531)
                     /locus_tag="VFR82_05155"
     CDS             complement(103..531)
                     /locus_tag="VFR82_05155"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEU4501019.1"
                     /translation="MTMKIPFTGGCVCGAIRYECTADPIMMFKCHCRDCQRVTGGGFV
                     AGVLVPASAFHVTKGQLRYHFTQSEAGGKHKRGFCPECGSRLTGGESDERATGFVGVT
                     VGSLDDPSWFRPQMDFFVSDTQPWDHMDQAIPKYDRYPPV"
     gene            complement(739..2877)
                     /locus_tag="VFR82_05160"
     CDS             complement(739..2877)
                     /locus_tag="VFR82_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501020.1"
                     /translation="MPAPLFLLLCMALVSASCGGGDKHLPSSNPPEYDPKKVYTAPAA
                     PPSAPAKVVKPTELELLRSKLDSLEVGQKVKGEGKKVPFDPNSLQLFKGVTNPCEALS
                     KVAQGLGSTQLFAGNEGAALKKALAPDADGIARRMDEQLAEAFQHSLGPGAADCPILV
                     RPEKKSGLSQPARLVLAHATSTLPVLLAQTTIPDTPPDDYEVQKNSSRENPPPDWVGW
                     KTTDTMTRIGKEERPTKGIREDYEMIIAPKAKRCPNPAGIVDGIFDWSFMMFRATPEA
                     QSVLYRRKVRATLKGEVGDDAQLKKVVKFDAILTLQHIGTELSHYSESFGIQGEFGID
                     QRRMGIPQDLKIITVSGFSEGEAQTKDAQLLGTLAALMAYFSGQEYFNAQTEWNRPNT
                     CVEIAFTPATKTKKFVPSESTPVKTELRTKKERAIVPAKFNEAKERPREGNGRVSPRE
                     KESQPNTPATFTYRAPATRVKHSGFWLVAVSRAGIAQAKDGEWELAPSSYVLEFKSHI
                     VQEALNLVNPQFGMFMSSNGFDAHVEATVPLQHRDDGQWVGEGVMQYATRTMTQPAQC
                     EIRIQGTGTTTFHVNGGSISMDPEPFAVNLVILPGQSGEVAETHCTSGNTPEKLKELL
                     ATQGVQGGDAHTVTKGGGWSGAFNFTRFRTFNWNKNGYEIGGWTQVLNSDVVAKKTMR
                     INCSIGLSACQEETTLILRMADEPGAEASP"
     gene            complement(3243..4118)
                     /locus_tag="VFR82_05165"
     CDS             complement(3243..4118)
                     /locus_tag="VFR82_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501021.1"
                     /translation="MTRIGMRLALTVAWVVAVASSVDWAHAQIAPQPACQNVMAPRTT
                     VFFVNGITTTLDEARLNAGKLELEFLNRLPSMSAAVQANCHVFSLNYNPTGGEVNDFF
                     EAAQQQLDITPTRFWLELEGLSLFTRELIRDALEGPMTDLNRIDASTIERHAVAYREQ
                     IASPSCRRVLIVPHSQGNLYTNAAYDLVFNQPPSPPPGAIKIVGVATPAQTVPGNGLY
                     RTSTTDVLINAIRLIRPGTLPPNTNWGITPLLVSPSYSGGHSFIGYLSADPSRTQILS
                     DIESSLTALTAVNPC"
     gene            complement(4115..4582)
                     /locus_tag="VFR82_05170"
     CDS             complement(4115..4582)
                     /locus_tag="VFR82_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501022.1"
                     /translation="MIVLVVVVWLAVGLTSCSGDSSDIPPPAAQETVAGIDANGDGVR
                     DDIEVYIDATYPVSAQGDTNRALRQFARAAQVTMIDASEPAPSVAHAAERFRALECLM
                     ARRPVDFHLIFVELRARVLNSTARSLAYLQADNHVRAGNVPLLPADQWRGACL"
     gene            4923..5318
                     /locus_tag="VFR82_05175"
     CDS             4923..5318
                     /locus_tag="VFR82_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HEU4501023.1"
                     /translation="MKEKKHIPTSDELFAWKSPFAALKDVTLPSTRTQPPARDLADKT
                     PAAPKSNRGRVDIIRSTAHRGGKTVTVVTGFVGIGQPEKERLAKAMQKACGAGGTVKE
                     GRIEIQGDHRDTVARILTNAGFRPVFAGG"
     gene            complement(5527..5988)
                     /locus_tag="VFR82_05180"
     CDS             complement(5527..5988)
                     /locus_tag="VFR82_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HEU4501024.1"
                     /translation="MKTDLEKVKRLAKDLREGYPRSPREKLGGYVIAARCADKCRAFL
                     LGMNGEYNYWPCSLASEWFAFTGIAPDQFKDVVATGATDEELARWVGEHSKIADSDEV
                     LKWNNRMRDMRLGEMSLHAQQYLEEYIPKFVPNHRPVYVWFDVYDLEEKRF"
     gene            complement(6216..6605)
                     /locus_tag="VFR82_05185"
     CDS             complement(6216..6605)
                     /locus_tag="VFR82_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU4501025.1"
                     /translation="MEPISALQLWFGRVLSTLAVLFLFVDAIGKLLRVGPVVEGTVKL
                     GYQENAVFPLGVLLLIGVVLYAMPRTSLLGAIYLTAFLGGAVATHVRVGSPLATHVLF
                     GVYVAVFLWGGLALRSPRLLTLLTGVH"
BASE COUNT         1426 a         1975 c         1844 g         1434 t
ORIGIN      
        1 ctcaaggagc agcatggcgc ggaggcactc agcggaacgg agagagaacc ttctaggtca
       61 tctccgcaag ccgtgatcag actgattcaa ggtttcatgc ttttatacag gtgggtaacg
      121 gtcatatttc ggaatagcct gatccatgtg atcccatggc tgcgtgtctg agacaaagaa
      181 gtccatttgt ggacgaaacc aactcgggtc gtcgaggctg cccacggtta ctccgacaaa
      241 accagtggct cgttcatctg actctccgcc ggtgagccgc gagccgcatt cagggcagaa
      301 gccacgcttg tgtttgccgc ccgcctcgct ctgcgtgaag tggtagcgca attggccttt
      361 tgtcacatga aaggctgatg cgggcacaag cacgcccgct acgaatccgc ccccggtcac
      421 tcgctggcaa tcgcgacagt ggcacttgaa catcataatt ggatcagccg tgcattcgta
      481 acgaatagca ccgcacacgc accctccggt aaagggaatc ttcatggtca tacgttatcc
      541 tcgtttcagt agccaaaacc agcaacaagg ccgaaggccc gggcatcccg ggtcgtgtgt
      601 tgattttcct tgggagcaga atcggtgtca ggaactacat cgcgacgtgt ggctggcatc
      661 gctgcctctg gatcgcacat cccacgccgt tctgcccaga cgctcgaaac cttcctcaga
      721 accccggata atttatttct acggagatgc ttctgcacca ggctcatccg ccattctcaa
      781 gatcagcgtc gtctcttcct ggcatgcgct cagtccgatg ctgcagttga ttctcatcgt
      841 cttttttgcc acgacatcgg aattgagcac ctgagtccag cctccgatct cgtagccgtt
      901 cttattccaa ttgaatgtcc tgaagcgagt gaagttgaag gccccgctcc atcccccacc
      961 tttagtcacg gtatgcgcgt caccgccttg tacaccctgt gtcgcaagca actcctttaa
     1021 tttttcgggc gtattgccgc tagtacaatg ggtctctgcc acttcccctg attgcccagg
     1081 cagaataacc aagttcacgg caaatggctc agggtccatg ctaatcgagc ctccgttcac
     1141 atgaaaggtt gtcgtgccgg tgccctgaat acggatttcg cactgagccg gctgagtcat
     1201 tgtgcgagtc gcgtactgca tcacgccttc cccaacccat tggccatcgt ccctgtgctg
     1261 gaggggcacc gtcgcctcga catgggcatc gaacccattc gaactcataa acatgccgaa
     1321 ttgcggattc actagattca acgcctcctg aacgatgtgc gacttgaatt caagaacata
     1381 agaagagggg gctaactccc attcaccgtc cttggcttgc gcgatgcccg cgcgggagac
     1441 cgcaactagc cagaagccgc tgtgcttcac gcgcgtggcc ggagcccggt aggtgaacgt
     1501 cgccggagtg ttgggctgcg actctttctc cctaggcgac acacgaccat tgccctccct
     1561 tggccgctct ttcgcctcgt tgaactttgc gggaacgatc gcccgttcct ttttggtccg
     1621 cagctccgtc ttcactgggg tcgattcgct tgggacaaac ttttttgttt tggttgccgg
     1681 cgtgaaggcg atctcgacac aggtgttggg cctgttccat tcggtttggg cattgaaata
     1741 ctcttgacca gagaaatagg ccatcagggc cgcgagcgtt ccgaggagct gcgcgtcttt
     1801 ggtctgagcc tcgccttccg agaagccgga gaccgtgatg atctttaaat cctggggtat
     1861 ccccatcctg cgctgatcga taccgaattc accctggatc ccgaatgatt cggaatagtg
     1921 cgacagttcc gtgccgatat gttgaagcgt gagtatggca tcaaatttca ccaccttttt
     1981 caactgagca tcatcgccga cctccccctt gagtgtggcc cggacttttc ggcgatagag
     2041 caccgactgc gcttccggcg tcgcccgaaa catcatgaag gaccagtcaa atatcccatc
     2101 gacaatccct gctggatttg ggcaccgctt cgccttcgga gcaatgatca tctcataatc
     2161 ttctcgaatg cctttcgtag gccgttcctc cttgccaatg cgcgtcatgg tatcggtcgt
     2221 cttccagccc acccagtcgg gaggaggatt ttctctactc gagttctttt gcacctcgta
     2281 gtcatccgga ggtgtgtcgg ggatcgtggt ctgcgccaag aggaccggta gggtggaggt
     2341 ggcgtgagcc agcacgaggc gagctggctg agaaaggcca ctcttcttct ctggccgcac
     2401 caagatcgga caatccgccg cgcctgggcc gagcgaatgc tggaaagcct ccgccaattg
     2461 ctcatccatt cgacgcgcaa tgccatctgc atctggggcg agggccttct tcagcgccgc
     2521 accctcattg cctgcaaaga gctgagtgct tccaagaccc tgtgccactt tcgacaaggc
     2581 ctcgcaaggg ttggtgactc ctttgaatag ttgtagcgag ttcgggtcga acggtacctt
     2641 ctttccctcg ccctttacct tttggccgac ttcaagcgag tcgagcttgg accggagcaa
     2701 ttccagttcg gtcggtttga caaccttggc cggcgcgctg ggtggcgcgg ccggtgccgt
     2761 atagactttc ttaggatcgt actcgggtgg attcgaggac ggcaggtgtt tgtccccgcc
     2821 cccgcaactg gccgacacta acgccatgca gagtagcagg aacaaggggg ctggcattcg
     2881 cggtctccgg gtgagggacc actcaacggc tggaatggcc gccatgctag ccgacgcctt
     2941 cggccacttc aatacccacc aaagtgtcgt tgctgcgttc agggatacga agatggaaga
     3001 gcggtgtcgt cagcccgttc cgcggcgcgg ggcggaccgg cttgtttctc acctggctgt
     3061 tctctcgccg ctcatcgcac ctcacagcgc gatagacttc cacccacgtt cgactgtgtt
     3121 cttatcttcc ttgtgctgcg aatccatcaa ggacgaggtc gaggacgagg caaaacatca
     3181 tcaccggtat cgggtctact ataagtcgct tcccgttcaa ccgaggaatg gataccgctc
     3241 cgctaacagg gattaaccgc ggtcaaagcg gtcagggaag actcgatatc ggacaagatc
     3301 tgcgtgcggc tggggtcggc ggagagatag ccaataaagc tgtggccgcc cgaataggat
     3361 ggagaaacga ggagcggagt gatgccccag ttcgtattgg gtggcagcgt tcccggcctg
     3421 atgagtcgaa tcgcattgat cagcacgtca gtggtcgaag tgcggtagag cccattgcct
     3481 gggaccgtct gggccggcgt cgccacaccg acgatcttta ttgcgccggg gggcggactc
     3541 ggcggctgat tgaacacgag gtcgtaggcg gcattggtat agaggttgcc ctgggagtga
     3601 ggcacgatga gcacgcggcg gcaggacgga gatgcgatct gctcgcgata cgccacggcg
     3661 tggcgctcga tggtcgaggc gtcgatccga ttgagatcgg tcatcggacc ctccaaggcg
     3721 tcgcggatga gttcccgcgt gaagaggctc agaccttcca gttccagcca aaacctcgtt
     3781 ggcgtgatgt ccagttgctg ctgcgccgcc tcaaagaaat cgttcacctc gccgccggtc
     3841 ggattatagt ttaacgaaaa tacatggcag ttcgcctgta cggccgccga catgctcggc
     3901 agccggttca ggaattccaa ctcgagtttg cctgcgttca atcgcgcctc gtcgagtgtc
     3961 gtcgtgatgc cattgacgaa gaacaccgtc gttctcggtg ccatcacatt ttggcaggcc
     4021 ggttgaggcg cgatctgggc atgcgcccaa tccaccgacg acgccaccgc gactacccat
     4081 gccacagtca aagcgagccg cattcctatc cgagtcatag acaagcgccc ctccattgat
     4141 cggccggcag tagcggaaca ttcccagccc tgacgtgatt atccgcctga agatacgcga
     4201 gcgaacgggc cgtcgaatta agcacgcggg cgcgcagttc gacgaagatg agatgaaaat
     4261 cgacaggccg gcgtgccatc aggcactcca atgcgcgaaa gcgctcggcc gcatgcgcca
     4321 ctgagggtgc aggctcgctc gcatcgatca tggtcacctg cgccgcccgg gcaaattgtc
     4381 ggagcgcccg gttcgtgtcg ccttgcgccg aaacgggata cgtcgcgtcg atatacactt
     4441 cgatgtcgtc acgaacacca tctccgttgg catcgatgcc ggcgacggtc tcttgggcag
     4501 ccggcggagg aatgtcggac gagtcgccag agcaggatgt gagtccaacg gcaagccata
     4561 ctacgaccac caaaacaatc attgcgcctc cacccccgcc gctgtcgttc aaccataata
     4621 agcgtttcgc cgtagcatta tatcgtatgg cgccaccggc ggcctttcgg aggctcgctg
     4681 acggagttgc cactagccat atcttcgtgg ggacgttcga attgcacgac acgtgtgggc
     4741 cggctcatcg cacctcacac ggcgatggaa cccgctcacg tggaaacgta gaatgttggt
     4801 tattcgaccg aagtcgatcc agacgattcc ggaatcactt cgcggcgcgt ggcgtccggt
     4861 tccaagactg cttgtagaat ctcctacttc aagctaacct tgtcaccctg gggacacacc
     4921 gcatgaagga gaagaaacac attcccacga gtgatgagct gttcgcatgg aagagtccgt
     4981 ttgctgcgct gaaggatgtg acactgccgt cgacacgaac ccaaccacct gctcgcgatc
     5041 tcgctgataa gacaccagcc gcaccaaaaa gtaatcgtgg tcgagtggat atcattcggt
     5101 cgaccgcgca ccggggtggg aagacagtga cagttgtcac gggatttgtc ggcatcggtc
     5161 aaccagaaaa agagcggctg gcgaaagcaa tgcaaaaggc ttgtggtgcc gggggcaccg
     5221 tgaaggaggg acggattgaa attcaaggcg atcaccgtga cactgtcgca cgcatcttga
     5281 ccaacgcagg attccgtccc gtcttcgccg gtgggtgagc cactctcccc taccctctcc
     5341 cgagacagaa aggggggacg tcgaaaaaca tgcgcctata aggaaccatt tcgcggcgtg
     5401 tggcgggcca ggtaatcggc tggtctccgt taaacgtctc cagtccgtgc actagggggg
     5461 agcaacccac tgattattca tcagtgggca actttcagag gtgacgagct gcccactcaa
     5521 gcgcgatcaa aaccgttttt cttccagatc gtatacgtcg aaccagacgt acaccggacg
     5581 atgatttgga acgaatttgg gaatgtactc ttctaagtac tgttgggcat ggagactcat
     5641 ttcaccgagt ctcatgtcgc gcatgcgatt gttccatttt aggacttcgt cggagtctgc
     5701 gatcttggag tgctcgccga cccatcgggc gagttcttcg tctgtcgcgc ccgtcgccac
     5761 cacgtccttg aactgatcgg gcgctatacc cgtgaacgcg aaccactcac tcgccaatga
     5821 acaaggccaa tagttgtact caccgttcat gcccaacagg aacgcccggc acttatctgc
     5881 gcagcgagcc gcgatcacgt accctcccaa cttttccctt ggactgcgtg ggtaaccttc
     5941 ccgaagatcc ttagccagcc gcttcacctt ctcaagatcg gtcttcatgc tcgcctcctc
     6001 aactgagcgg atagtttgct tccgccaagc ttgctccttt ctggttcaaa ctttcaatca
     6061 gggaggaaaa atgaccttct gcttcttcag aaaaatgtac cctttgacgc catcacgcga
     6121 cgcgtggcgg gaaggcgagg atcaagccgg ccagccgcac cactcatcgc acctcacacc
     6181 gcaacagacc cggcactcaa gcagcgcccc aggtatcagt ggacacccgt gagcagggtc
     6241 aatagtcgtg ggctgcgcag tgcgagtcca ccccacagaa ataccgctac atacactccg
     6301 aacaacacgt gagtagctag cgggctgccc acgcgtacat gtgttgcgac agcaccgccg
     6361 aggaaggcag taaggtagat agctccgaga agcgaggtgc gtggcatcgc atagagcacc
     6421 acgccgatca acagaagcac gcccaaagga aacacggcat tctcctggta tcccaacttc
     6481 acagtccctt cgacgactgg cccaactctc aggagctttc cgatagcgtc aacgaacaag
     6541 aacaggaccg ccagcgtact cagtactcgt ccaaaccaaa gttgaagtgc tgaaatcggt
     6601 tccataaggc acctccgaat gagttagcaa ggagaaaacc tgcgtacctg tacacgccat
     6661 attgagtgta gtaaactac
//
LOCUS       DASTQY010000159         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1186761, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000159 DASTQY010000000
VERSION     DASTQY010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186761"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..512
                     /locus_tag="VFR82_05190"
     CDS             171..512
                     /locus_tag="VFR82_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU4501026.1"
                     /translation="MPIYEYRCAECHKQSSFLVLSPSTESSLRCSHCQSLRLERLLSR
                     FASPKSEEARMESLADDSALAGLDENDPGNMERLMKRMGNEMGTEVGDDLEALENSDE
                     SVGNPDGFDSL"
     gene            834..1274
                     /gene="mraZ"
                     /locus_tag="VFR82_05195"
     CDS             834..1274
                     /gene="mraZ"
                     /locus_tag="VFR82_05195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEU4501027.1"
                     /translation="MFAGEYLCKVDEKGRFIVPSPIREQVDSEGQAVVFLKGPEESIL
                     IYSMKEWEKVLERTKTTLDEEQSRLFMHFVVSEAGTSELDKAGRILIPGKLRKLVPLD
                     DDQEIILVGLYHRLEIWNPSEWRRYIARSEDRYEQNMAKILNLL"
     gene            1337..1879
                     /locus_tag="VFR82_05200"
     CDS             1337..1879
                     /locus_tag="VFR82_05200"
                     /inference="COORDINATES: protein motif:HMM:NF017663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEU4501028.1"
                     /translation="MPGYRRPIRVPASSLPPTLISFRTSRSIRRPAQPQAALNHLNAD
                     ALSSLHEEQRSIALTLPVPPSINHQYATVNGRRLLSSTGRSYKAQVGQLVWVRLAASP
                     HRTVLLSQLQSCALALSIRFYFTSALRRDVDGGLKIAQDAICEGLGLNDNRIIETHLY
                     KHVDRRNPRIEVVLSATSLA"
     gene            complement(1889..>2912)
                     /locus_tag="VFR82_05205"
     CDS             complement(1889..>2912)
                     /locus_tag="VFR82_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4501029.1"
                     /translation="VSGVRGGQGGAVTLTVSDALDLKKKIPLLSETGWARREVMQMVN
                     GNRNWNGPINGVSPSYLTIRDWAFSSGGPFTQADLDSAARVALIGQTIVDNLFDEGEE
                     PVGAVIRIRNVPFRVIGVLSPKGQSAQGSDQDDVIFIPFTTAERKVFGTMFLGSVAAL
                     FASTDRPEDLPEAVERIREVLRGRHRLQAEQADDFTVKTQVDIGKVQEGTSYTLTLLL
                     FSVASVSLLVGGIGIMNILLVSVTERTREIGVRMAVGAKRRHIVMQFLIEAMTLSLLG
                     GAVGVVLGVVGAKLTTVVAGWPTVISTDVILLAFLFSLAVGLFFGLYPANKASRLNPI
                     EALRYE"
BASE COUNT          662 a          877 c          774 g          599 t
ORIGIN      
        1 ctataccacc ggaacgacgc tgaacgagtg cgccaagacg ctgctggcgg ccggggccct
       61 ctcagtctcg gagctcacgc tcgccaggac acttgactcg agccttgtac cagaccgtat
      121 ttgggccgac cacgctgacc gctcgttgac aggattcgga ctttgacagg atgccgattt
      181 acgaatatcg ctgtgccgag tgccacaagc agagcagttt tctcgtcctg agcccctcca
      241 ctgaaagctc gctccgctgc tcccattgtc agagtctcag actcgaacgg ctcttgtcgc
      301 gcttcgcgtc gccgaaatcg gaagaggcgc ggatggaatc cttagccgac gacagcgcac
      361 tggccggtct tgatgagaac gatcccggga acatggaacg tctgatgaag aggatgggca
      421 atgagatggg aacagaagtc ggggatgatc tggaagcctt ggaaaattct gacgaaagcg
      481 tcggcaatcc ggacgggttt gacagtctat gaccggcatt gtcatacaaa ttccttgaca
      541 gatgtcgttg atttaattac cctggcccca attggagtga taggtattat ggtgccggcg
      601 agccgatggc ggatgttcaa tgaccaaagc gcccaagagc gagctcatga ccgcggaaga
      661 gacctgccgc tatctcaaaa tcacttccag aacgctgtac cgctatcttc acagtcatca
      721 aattcccgct ttcaaacttg ggaaagaatg gcgattcgtc cgttcagatt tagaacagtg
      781 gattcgagac cggactcgga gacccgtctc ctcgtgaagg agcgcattcg ctgatgtttg
      841 cgggagagta cctctgcaaa gtcgacgaga aaggacggtt catcgtccca tcccccattc
      901 gtgagcaagt cgactcggaa gggcaggcag tggtattcct caaaggcccg gaagagtcca
      961 tcctgatcta ttcgatgaag gaatgggaga aggtgctcga gcggacgaaa accaccctcg
     1021 atgaagagca gagccggctg ttcatgcact tcgtcgtctc cgaagcaggc acgtccgagc
     1081 tcgacaaggc cgggcgaatc cttattccag ggaagttgcg aaagctggtg ccgctggacg
     1141 atgatcaaga aatcatcctc gtcggactct accatcgcct ggaaatttgg aatcccagcg
     1201 agtggcgccg ctatatcgct cgatccgaag atcgctatga acaaaacatg gcgaaaatcc
     1261 tgaatctcct ctaggcacgc gttgaaccat cctacgactt cccgacctag gccgcttcga
     1321 gacagagcgt gaactgatgc ccggctatcg gcgccctatc cgagttccgg cttccagcct
     1381 tccgcccact ttgatctctt ttcgcacctc gaggagcatt cggcgtccag cgcaaccgca
     1441 ggctgccctg aatcatctga atgctgacgc cctctcgtct cttcacgaag agcaacggtc
     1501 catcgcgctc acgcttcccg ttcctcccag catcaaccat caatacgcca cggtgaacgg
     1561 gcgccgtctg ctctcatcga ccggccgctc gtataaggca caggtcgggc aactcgtctg
     1621 ggtacgactc gcagcatctc ctcataggac cgttctcttg tctcaactcc aatcctgtgc
     1681 gctcgcctta tccatccgct tttattttac gtcggccttg agacgtgatg tggatggcgg
     1741 gctgaaaata gcacaggatg ccatctgcga aggtctgggg ttaaacgaca atcggattat
     1801 tgaaacgcat ctctacaaac acgtcgaccg gagaaatccg cgcatcgaag tggtgctgtc
     1861 cgccacttcg ctggcttgac cgtacggttt attcgtatcg gagcgcttca atagggttga
     1921 gtcgagacgc cttgttggcg ggatagaggc cgaaaaacag tccaacggcg agggaaaata
     1981 ggaaggccaa caggatcacg tctgtcgaaa tgacggtggg ccacccagcc actacggtcg
     2041 tcagtttcgc tcccacgacg ccgagcacga cacccactgc tcctcccaag agactcaagg
     2101 tcatcgcctc aatcaggaat tgcatcacga tgtgacggcg cttggcgccg accgccatcc
     2161 ggacaccgat ctctcgtgtc cgttcagtca cagacactag cagaatgttc atgatgccga
     2221 tcccccccac cagcaaggat acggaagcca ccgaaaacag caacagggtg agcgtgtagc
     2281 tcgttccctc ctggactttt ccaatgtcga cctgcgtttt caccgtaaaa tcgtcggcct
     2341 gctcggcctg aagccgatga cgtccacgca acacctcgcg aattcgctcg acggcttccg
     2401 ggagatcctc tggccgatcg gttgacgcga acaaggctgc gaccgagccc aagaacatcg
     2461 tgccgaagac tttgcgctcc gccgtcgtaa atggaataaa gatgacgtca tcctgatcgg
     2521 atccctgcgc cgactggccc ttgggcgaaa gaacaccaat gacgcggaac gggacattcc
     2581 tgattcgaat cactgcaccg accggttctt cgccttcatc gaacaggttg tccacgatgg
     2641 tttgaccgat caacgccacg cgggcggcac tgtcgagatc cgcctgggtg aaaggccccc
     2701 cgctcgaaaa cgcccaatcc cgaatggtga gatagctcgg cgaaacgccg ttaatcggac
     2761 cattccagtt ccgattcccg ttgaccatct gcatgacctc tcgccttgcc catcccgtct
     2821 ccgacagcaa cggaatcttt ttcttgagat ctaacgcatc cgagaccgtg agggtcacgg
     2881 cgccgccttg gccgccgcgc accccgctca cc
//
LOCUS       DASTQY010000160         4539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_40278, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000160 DASTQY010000000
VERSION     DASTQY010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40278"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VFR82_05210"
     CDS             <1..406
                     /locus_tag="VFR82_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU4501030.1"
                     /translation="FVVVMGTLWSLIIATDNGVRTIPPIFRRAARTMGSTGLHTWTHV
                     ILPAALPFLISGMKQGWAFAWRSLMAAEIYVTILSGFGLGQLLHYGRELHAMDQVIGV
                     IIVIVLIGLAVDKSLFAPTERFMYRRWGTERA"
     gene            463..1977
                     /locus_tag="VFR82_05215"
     CDS             463..1977
                     /locus_tag="VFR82_05215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4501031.1"
                     /translation="MNRSLTGWFRHLSISQKLLISFGLILSLLALSLTALLFYLARIN
                     SYVDRHKRITVPAIVTAANMRRDAYDVKLALHSPSRTEIAGRLAQHASQLTSALNLYK
                     GTHAARTHPILYGMLAEHRRLDLAEQEDRAIEDIAQSLATLTTNLHHLDRSEADGLIT
                     RVTDGLDQLMDAHTQIDVEMKHEGDRLVGRARLIALSLALLLGLVIAITYVLAARHVV
                     RPLMTLAATADRVAHHDFSARFPAWPSRDEVGTLAASLTTMLASLREHSTSLLRKTKE
                     LEAFTYSIAHDLKGPLREIEGFSSLLEKHFAEGGDQQVRHHIKVIRHSALRLTHMIDA
                     LLKYSRLEQQELPKQQFNVRDMITNLLNDRVGFTQNPKPKIELDLQLDDVYGEPVSIR
                     QALANLLDNAIKFSRLRAAPEIRISGSRTENEYVLSVQDNGIGFDMAQTDKIFGLFER
                     LHGPEDYEGTGVGLAIVRLVMDKHGGRVAVESAPGKGSTFTLTIPINHADSGRS"
     gene            2031..3368
                     /locus_tag="VFR82_05220"
     CDS             2031..3368
                     /locus_tag="VFR82_05220"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU4501032.1"
                     /translation="MRALIIDDEEYVRLVLGQTLREEGCDVVVATHGEAGIQLLQSQP
                     FDCVITDLRMPGVDGLAVLKWVADRQPEVDVVLLTGHGDVKDAVEAMKHGAWDFLIKD
                     TPFDGTAVKAVLAKLRTVRALRQENLAARRGGYTRDVIIEGSSPAWKKLRAQIVQAAS
                     SKAPVLIQGETGSGKEVVARLLHDLSNRARGPFLAINCGAMNRELLESELFGHEKGAF
                     TGAVGTKTGLVTTAEGGTLFLDELGEMPTQMQVSLLRFLDRGEYRPVGSTRTLQADVR
                     LVGATNQDINVLVTERRFRDDLLYRINAVTLHVPALRERQDDVIMLADHFLQSVRAAG
                     LSPRLLSPSAKAALSTYPWPGNVRELHNVIERLVMSGEGTDAISGDEVLAVLPKSQPG
                     LQLQQLSQCSLEDVERVHILRVLEVSGGNKTHAAKILDIDYKTLLAKLKKYGL"
     gene            3431..4033
                     /locus_tag="VFR82_05225"
     CDS             3431..4033
                     /locus_tag="VFR82_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501033.1"
                     /translation="MPRPSRLSFLIGCSIMIPALALSADKPPQKGSTVKEVKTMSEKD
                     QTALALSAAPAHISKDASVMIYGTDGKLTEVRKGTNGFTCIPTVMNLPVPDPICMDGA
                     SNQWMTDIMNNAPKPTNTVPGIAYMARGGSHLEKGGKVVMSGDGAKVVKEPPHWMIMW
                     PFDASASKLPTAPNPSGVYVMFEDSPYAHIMVYQDPNKMK"
     gene            4113..>4539
                     /locus_tag="VFR82_05230"
     CDS             4113..>4539
                     /locus_tag="VFR82_05230"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEU4501034.1"
                     /translation="MNVRRTEERAEDIARVLDSALSIPGWRGKVGVDPLLGLVPVVGD
                     VLVTVSGAAILVMARQLHVPWNMQLRMAYNLLKNGVIGAVPFIGDVYSFFFKSHQLNA
                     ALLVRSVKRGEDGQCQLTTTALSIRDMTALALLIAPTVLL"
BASE COUNT         1011 a         1287 c         1285 g          956 t
ORIGIN      
        1 gtttgtcgtg gtgatgggga cgctgtggtc actgatcatc gcgacagaca acggtgtacg
       61 gaccattcct ccgatcttcc gccgggcggc ccgcaccatg ggttcgaccg gcttgcacac
      121 gtggacccat gtgatccttc cggcagccct tccatttttg atcagcggga tgaaacaagg
      181 ctgggcgttt gcctggcgtt cgctgatggc ggcggaaatc tacgtaacca ttctgagcgg
      241 cttcggtctc ggtcaacttc tccactatgg ccgggaattg catgccatgg accaggtcat
      301 cggcgtcatt atcgtcatcg tgctgatcgg cctcgcggtt gacaagtcac tgttcgcccc
      361 caccgaacga ttcatgtacc ggcgatgggg gaccgaacgc gcgtaacgga cgcgtgcttc
      421 ctctcactgg cacccgcatg gtgcctatgg taccgtagcg ccatgaaccg ctctctgacg
      481 gggtggtttc gccacctttc gatcagtcaa aaacttctca tctcgttcgg attgatcctg
      541 agcctcctgg cattaagcct cacggcgctg ctcttttatt tagcccgtat caatagttat
      601 gtggaccggc acaaacggat tacggttccc gccatcgtca ccgccgcgaa catgcgacgg
      661 gatgcctacg atgtgaagct ggctttacac tcgcctagcc gtaccgagat cgccggccgg
      721 cttgcacaac atgcttccca gctcacaagc gcactcaacc tgtataaagg cacgcacgcc
      781 gcccgtaccc accccatttt gtatggcatg cttgcggagc atcgccgcct cgatctggcg
      841 gaacaggaag atcgggcgat cgaagacatt gcccagtccc ttgcaacatt gaccacaaat
      901 ctgcaccacc ttgacagatc ggaagccgac ggcctgatca cgcgtgtcac agatggtctc
      961 gatcaactga tggacgctca cacgcagatt gatgtggaga tgaagcatga aggcgatcgg
     1021 ctggtcggcc gcgcccgctt gattgccctc tcgctggctc tcttactcgg ccttgtcatc
     1081 gccatcacct acgtgctcgc ggcaaggcac gtcgtccggc ctctgatgac gctcgccgcc
     1141 accgccgacc gggtggctca tcacgatttc tcggcccgat ttcccgcatg gccgagccgc
     1201 gacgaagtcg gtaccctcgc cgcctccctc acgacgatgc tggccagtct acgggaacac
     1261 agcacatcgc tcctgcgcaa aacgaaagaa ttggaggcct tcacctattc gatcgcccat
     1321 gatctaaagg gaccattgcg tgaaattgaa gggttttctt cattgctcga aaagcacttt
     1381 gccgaaggcg gcgatcagca agtgaggcac catatcaagg tcatcagaca ctcagcgctc
     1441 cgccttacgc acatgatcga cgccctcctc aagtattcgc gtttagaaca acaggaattg
     1501 ccgaagcaac agtttaacgt tcgcgacatg atcacgaacc tgctgaacga ccgagtaggt
     1561 ttcacacaga acccgaaacc aaagatcgaa ctcgacctcc agctggacga cgtgtatggg
     1621 gagccggtca gcatccgcca agcgctcgcg aacctgctgg ataacgccat caagttctca
     1681 cgtctccggg ctgctccgga aatacgaatc agcggcagca ggaccgagaa cgagtatgtc
     1741 ttgtctgtac aagataacgg catcggcttc gacatggctc agacggataa gatcttcgga
     1801 ttgttcgaac gtttgcacgg tccagaagac tatgagggaa ccggagtcgg tttggcgatc
     1861 gttcgactcg tcatggacaa acatggaggc cgggtggcgg tggaatctgc gcccggcaaa
     1921 ggaagcacct ttacgctcac gattcccatt aatcacgctg attccggaag gtcgtaggcc
     1981 ccaagatagc ggttgagtct actacatccc gacggagaca cgccgcgagc atgcgcgcgc
     2041 tgattatcga cgacgaagaa tatgtccggt tggtcttggg gcagacgctt cgggaggaag
     2101 gatgtgatgt cgtcgtcgcc acgcacggtg aggcggggat ccagttgctt caatctcaac
     2161 cctttgactg tgtgattacc gatctacgga tgccgggtgt tgacggactg gccgtcttga
     2221 agtgggtcgc agatcgccaa cccgaggtcg acgtggtgtt gttgacaggt cacggagacg
     2281 tgaaagatgc cgttgaggcg atgaagcacg gcgcatggga ttttctcatc aaggacacgc
     2341 cgttcgacgg cactgccgtc aaagcggtgc tggccaaact gagaaccgta cgagcgttac
     2401 ggcaggaaaa cctcgcggcg cgccgcggcg gatatacacg ggacgtcatc attgaaggat
     2461 cgagcccggc gtggaagaag ctgagagctc agattgtaca agccgcatca tcgaaagctc
     2521 cggtcctcat ccagggagaa accggctccg gcaaggaagt ggtggcccgg ctccttcatg
     2581 atctgagcaa cagagcgagg gggccttttc ttgccatcaa ctgcggagcc atgaaccgtg
     2641 aactgctcga gagcgaactg ttcggtcatg aaaagggagc gttcaccggc gcagtcggca
     2701 caaagaccgg cctggtcacg acggcggaag gaggcaccct ctttcttgat gaattgggag
     2761 agatgccaac ccagatgcag gtcagtcttc tccggtttct ggatcgaggc gaatatcgac
     2821 cggtcggcag cacccggacc ctgcaagcag acgtccgtct tgtgggagcg accaaccagg
     2881 acatcaatgt cctcgtgacc gaacgccgct ttcgagacga tctgctgtat cgtatcaatg
     2941 cggtgaccct gcatgttcct gcgcttcgtg aacgtcagga cgatgtgatc atgctggcag
     3001 atcatttcct gcagagcgtc cgcgcggcgg gactgtctcc tcgactgctc tcccctagtg
     3061 caaaagccgc actctcaaca tatccttggc cgggcaatgt ccgggaattg cacaatgtca
     3121 ttgagcggtt ggtcatgtcc ggggagggca cggacgcgat ttctggagac gaagtcctag
     3181 ccgtcttgcc aaaatcacaa ccaggcttgc agcttcaaca actttcacaa tgctcactgg
     3241 aagacgtgga acgcgtgcac attctccggg ttttggaagt gagcgggggc aacaaaacgc
     3301 atgccgcaaa aatattggac attgactaca aaacgcttct tgcgaaacta aaaaagtacg
     3361 gtctatgatt cgcggttcgt ctcagtcaga ttgattagcg ggaaattcac tttcatcaag
     3421 gaggatagcc atgccccgtc cgagccgtct cagtttcctc ataggctgtt cgatcatgat
     3481 acctgccctg gcgctctcag ccgacaagcc gccccaaaaa ggatcgacgg tcaaagaagt
     3541 caaaacgatg tccgaaaagg accagactgc gctagctttg agtgccgcac cggcgcacat
     3601 ttccaaggac gccagcgtga tgatctacgg cacggacggc aaactcacag aagtgagaaa
     3661 gggcacgaat ggatttacat gtatccccac tgtcatgaac cttcccgttc ccgatccaat
     3721 ttgcatggat ggagccagca accaatggat gactgacatc atgaacaacg ctcctaaacc
     3781 gactaatacc gtcccgggaa ttgcctatat ggcacgggga ggctcgcact tggaaaaagg
     3841 cggcaaggtg gtgatgtccg gtgatggcgc caaagtggtg aaggaaccac cgcattggat
     3901 gatcatgtgg ccgtttgatg cgtcagcctc gaagctgccg accgcgccaa atccgtccgg
     3961 agtttacgtc atgtttgagg atagtcccta cgcgcacatc atggtgtatc aggatccgaa
     4021 caaaatgaag tagtcggtgc tgtgcaagcg tgatcccgac gccatggccg aacaccgctg
     4081 ttcatgctgt cgaattgcgt cggaggcttc ggatgaatgt tcgccgaacc gaggagcgtg
     4141 cggaagacat tgccagagtg ctcgacagtg cactgtccat tcctgggtgg cgcgggaaag
     4201 taggagtcga cccgctcctc ggcctggttc cggtcgtcgg cgatgtcctt gttacggtga
     4261 gtggcgccgc cattctcgtc atggcacgac agctccacgt gccgtggaac atgcagctcc
     4321 ggatggccta caacttattg aagaacggcg tgatcggagc agtgccgttt attggagatg
     4381 tgtattcatt tttcttcaag agccatcagc tcaacgccgc gttattggtt cgttccgtga
     4441 agcgaggtga ggatggccag tgccagctga cgaccacagc cttgtcgatt cgggatatga
     4501 cggcattggc cctcctcatt gcgccgacgg tgcttcttg
//
LOCUS       DASTQY010000161         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1640874, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000161 DASTQY010000000
VERSION     DASTQY010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640874"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /gene="nuoD"
                     /locus_tag="VFR82_05235"
     CDS             <1..1173
                     /gene="nuoD"
                     /locus_tag="VFR82_05235"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEU4501035.1"
                     /translation="SRRREELLLNMGPQHPSTHGVLRVVLELDGERIVKATPDLGYLH
                     RGVEKLCEGLAYMQVIPHTDRLDYVCAMANNYAYVRAVEKLLGISIPERAEYIRTIVA
                     EMQRIVGHLFWLGTQALDIGAMTVFFWTFREREALLDMFEKLCGARLTLNYYRIGGVD
                     SDFTPDLVLRLKSFLDAFPEKVKEYDSLIASNRIWLGRTKHVAVISADDAINFGCTGP
                     VLRGSGVAYDVRKEEPYGVYDKVDWEVPIGKNGDTYDRYWVRMEEMRQSARIIRQCLD
                     QLPQGPIVADAPQYIPPPKQLVMRDMESLIHHFIIFTQGFKPPKAETYCASESPKGEL
                     GFFIVSDGSARPYRLKIRAPSFVHMGAFDHMARGYLISDIITIFGTYDIVMGECDR"
     gene            1424..1969
                     /locus_tag="VFR82_05240"
     CDS             1424..1969
                     /locus_tag="VFR82_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEU4501036.1"
                     /translation="MLRDRYQEEINEILSRYPVKRSALIPLLYLAQRENGYITEASMT
                     EIASLLKLTPPQVYETATFYTMLNLKQVGKFHIQVCKSLMCALVGSDAVVAWIKSKLG
                     IGPGETTSDGAFTLTLVECLAACGTGPMMQINDDYYERLTEDKFDRILADLKATGSSA
                     LKSGPFMWPDPLVMKSERPVR"
BASE COUNT          492 a          575 c          580 g          436 t
ORIGIN      
        1 tcccgccgcc gagaagagct gttgctcaac atgggccctc agcatcccag cacgcatgga
       61 gtcctgcggg tcgtgctcga attggatgga gagcgaatcg tcaaagcgac cccggacctg
      121 ggctacctgc accgtggtgt cgagaaactc tgcgaaggac tcgcctatat gcaggtcatc
      181 ccccacacag accgcctcga ctatgtatgc gccatggcga acaactatgc ctatgtacgc
      241 gcagtggaga aactgctcgg catttcgata ccggaacggg cggaatacat ccggacaatt
      301 gtggcggaaa tgcagcggat cgtcggccat ctgttttggc taggaacaca ggcactggat
      361 atcggcgcca tgacggtgtt tttctggacg tttcgagaac gcgaagcctt gttggacatg
      421 tttgaaaagc tctgcggcgc ccggctgacg ttgaactact accgaatagg cggcgtcgac
      481 agcgacttca ctcctgatct ggtcctgcgg ttgaagtcct tcctcgatgc ctttcctgag
      541 aaagtcaagg agtacgattc cctgattgcc tcgaaccgga tctggctcgg ccgtacgaaa
      601 cacgtggccg tgatctccgc cgacgatgcc atcaatttcg gctgcacggg gccggtgctg
      661 cgcggatccg gcgtggccta cgacgttcga aaagaagaac cgtacggcgt ctacgacaag
      721 gtggattggg aagttccgat cggtaagaac ggagatacct acgaccgata ttgggttcgc
      781 atggaggaga tgcgacagag cgcgagaatc atcagacaat gtcttgatca gctcccgcaa
      841 ggtccaatag tcgccgatgc gccgcaatat attccgcctc ccaagcagct cgtgatgcgg
      901 gacatggaaa gcctgattca ccatttcatc atctttactc aaggcttcaa accgccgaaa
      961 gcagaaacgt actgtgcgtc ggagtctcca aaaggcgagc tgggtttctt catcgtgagc
     1021 gacggcagtg cccggcccta tcgtttgaag atccgcgctc cgtccttcgt ccacatgggt
     1081 gcctttgacc atatggcccg aggctatctc atctccgaca tcattacgat tttcggaaca
     1141 tatgacatcg tgatgggaga gtgtgatcgg tgatgctcag ggagccaggt gtccgcagtg
     1201 ctcgcgaaag acggcacacc agaaagacgt gcggaagggc tgttgatctc ctttgctcgc
     1261 cgaacgcggc acacaggatc aatcgtattt gaaaatagag ggcggtgctg gtccgggcgc
     1321 gcagtagaag attgatcagc cctcgcccca atgaaagcaa gcgatcttgt agaccagttt
     1381 ctacaacaca ttggttccag cctcaccgat ttaggatggg cacatgttaa gagacaggta
     1441 ccaagaggaa attaacgaaa ttctcagccg atacccggtc aagcgatccg cgttaatccc
     1501 cttgctctac ctggcgcagc gtgagaacgg ctacatcacc gaagcatcga tgacggaaat
     1561 cgcctccctg ctgaagttga cgcccccgca agtctatgag acggcgacgt tctacaccat
     1621 gctgaatctg aaacaggtgg gcaagtttca tatccaggtc tgtaaatcac tcatgtgtgc
     1681 gctcgtcggc tctgatgccg tcgttgcctg gattaaaagc aagcttggaa tcggtccggg
     1741 cgaaaccaca tccgacggcg cgttcacatt gacactcgtc gaatgtctgg ccgcctgcgg
     1801 aaccggaccg atgatgcaga tcaatgacga ctattatgaa cgcctgaccg aagacaagtt
     1861 cgaccggatc ttggcggacc tcaaagccac tggctcgtcc gctctgaaga gcggcccctt
     1921 tatgtggccc gaccctcttg tgatgaaaag cgaacggccg gtacgctgaa gttatggcac
     1981 agcacgaacc tatcctcctc aaaaatatga gtcagcccgg gtataccggc tcgttggccg
     2041 actatgaaca gacggggggc taccaggcga tcaaaaaggt cat
//
LOCUS       DASTQY010000162         5831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1026077, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000162 DASTQY010000000
VERSION     DASTQY010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026077"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1433
                     /gene="larC"
                     /locus_tag="VFR82_05245"
     CDS             231..1433
                     /gene="larC"
                     /locus_tag="VFR82_05245"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEU4501037.1"
                     /translation="MSVHLHFDCFSGISGDMTLGALVDAGAPFGELVKRLKRLGVPGF
                     TLRKRRVQRGAISATKVDVVITSGLPRPWPLSRIRKTLSDSRLSSKIQQQSRAVFERL
                     ADAEAKAHRVARDHVHFHEVGLVDSFVDIVGGLICCDLLGVVRVTASAVNVGAGTLQS
                     AHGLLPAPGPAVAILAKGVPIYSAGPARELTTPTGLALLRTLTEDFGPMPPMKPVAVG
                     YGAGDANPEGWPNVLRVFLSRDAATGGRGRDTVVQIETNLDDVNPQTYEHIMERLFGA
                     GALDVALIPAIMKRGRPAIVLTCLASFTAVDSLLDVLFEETTALGVRVQEITRHILPR
                     QFTSVKIRGGTVRIKIAAVNQTSSKAAPEFIDCKRIAERTGRPVKAVLEEATLAYAKT
                     RSSAKDRL"
     gene            1430..2434
                     /locus_tag="VFR82_05250"
     CDS             1430..2434
                     /locus_tag="VFR82_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501038.1"
                     /translation="MTLLGYVLLFGASVVITLGACALFTNAIEWLGKRLNLSEGAVGS
                     VLAAIGTTLPETSIPIVAIFFGATREEAEVGLGAILGAPFMLSTLVVPLLAVLLLVYA
                     QLGKRGRSFRLNYKDVTVDLSCFALAYTVAMACTFVPSRTLRIIVAVGLLGLYSYYVK
                     IKFSDDDAGGEPSDLSPLVFAKNSARPSYPLIGLQAVAGLGGLALGAHLFVMAADSTA
                     HLLAISPLILALLVAPLATELPEMSNSFLWLYQKKDRLAVGNVTGAMVFQGTVPVSVG
                     LVGTEWTLAPSALLTMVLAVLATGSLLLQAVWSGHWRPWLLIIGAILYIGYAFYLYGY
                     "
     gene            2525..3559
                     /gene="pdxA"
                     /locus_tag="VFR82_05255"
     CDS             2525..3559
                     /gene="pdxA"
                     /locus_tag="VFR82_05255"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEU4501039.1"
                     /translation="MKKAQSPTRVPMLGITMGDPAGIGPEVIAKALAGTALRRICFPL
                     VIGSQEVMQRTVKQLKLKLAVRPVEGHDPIRLSAGHVALLDPLEQPLGPYTLGVAAAE
                     TGAASVAFIKTAVRLAQSECIDGMVTGPINKEAMNMAGCHFPGHTELLADLTQTPESG
                     MMIIGGRLKIMFVTTHIAIKELPAALTQAKIEKGIRLAHLALTTLFGIKQPRIGVAAL
                     NPHAGEHGLFGDEEIRLIGPAARAAQAQGILASDPLPADTLFGKTARGDYDAVVALYH
                     DQGLIPLKMVAFGTCVNLTVGLPIIRTSVDHGTAFDIVGKGIADPGSLIEAVKLAASL
                     ARRRTSHARA"
     gene            3575..3907
                     /locus_tag="VFR82_05260"
     CDS             3575..3907
                     /locus_tag="VFR82_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501040.1"
                     /translation="MPLDANHALLVIQQQLKELDAIYRDTLQMSLNTVAGTERVAKWR
                     VRTVSLLAESVGEKAAQDFARLQPGMVFTNDLVEEFTDLVDCFRVPLSALAKQLTQTA
                     QRPADGGS"
     gene            3908..4717
                     /gene="rsmA"
                     /locus_tag="VFR82_05265"
     CDS             3908..4717
                     /gene="rsmA"
                     /locus_tag="VFR82_05265"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU4501041.1"
                     /translation="MPGPAFPRASKRLGQHFLIDQNIVRKIVAAAELTPDDTVLEVGP
                     GRGILTEALASVVRRVVAVEIDPQLYRFLQARQSEWKNVEVVCADALSYSLEAVAAGA
                     VVVANLPYYISTPLLFRFLEHRHRFPRLVLMLQNEVADRLVAKAGTSAYGVLSVMGQY
                     AADISKAFTVSANCFRPRPEVGSAVVVLRTKHEALLSPDEEPAFAAVVKAAFAHRRKT
                     LVNSLRDEGYDTQAVIDAISAVGLVSTVRAETLSLDTFSRLARRLDRRKPS"
     gene            4780..>5831
                     /locus_tag="VFR82_05270"
     CDS             4780..>5831
                     /locus_tag="VFR82_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEU4501042.1"
                     /translation="MGVTSSAKRGEARRAPAPPSHLKREVIGVILIALSLLTLLSLLS
                     FVPGEPKVVATPAVTMTPPTHNLIGSVGAVFSSLLFSLIGGAAYLFPILLGLLGGRCF
                     TQSDLSIRLRNAGASLAALLFLSGLLHLEVIAVPTVSSGMVFRGQAGGLLGQLLAEGL
                     RAYFANTGTHILIISGFLVALLFTAPLSLAQLAVQAPQWGRNAFARLNALMPRREQAA
                     ATPDPPKPTKTRKAKSIRAVIEEVLPPAEDNPPAEVFSVTPPTVVLPEPREIVELEPD
                     APVVGKVEDTGDYQLPDADELLTEPSGILARITDEELKGQSDILSKALLSFGIEGRVT
                     EVRPGPVVTMYEFEPAP"
BASE COUNT         1108 a         1718 c         1752 g         1253 t
ORIGIN      
        1 agaccgattg ccggagtggg ataacacagt gcacagaagc tgtcaatgca cactcgtcat
       61 tgccctccca agggaccgtt gggtaagatg acgcgacttt taccaccagc acatgaacca
      121 tgtctcggcg gttgaagctg ggaacgttct actcaatcgt gacgcgaagg cggatcctag
      181 cgcacgagta tggtcacgta agatgagcgg actggtcgcg aaaggatggc gtgagcgtac
      241 acctccactt tgactgtttt tccggaatca gcggggatat gaccttgggt gcgcttgtgg
      301 atgcgggtgc accattcggc gagttggtca agaggctcaa acgactcggc gtgccgggct
      361 ttacactccg taaaagacga gtgcagcgtg gcgccatttc tgcgacgaaa gtagacgtcg
      421 tcatcacgtc cggtcttccg cgaccctggc ccctgagccg tatccggaag acactttccg
      481 acagccggct ctcttcgaaa atccagcagc aaagccgagc ggtattcgag cggctagccg
      541 acgcggaggc caaggcccat cgcgtcgcaa gagaccacgt gcatttccat gaagtcggat
      601 tggtggattc gttcgtcgat atcgtgggag ggctcatctg ttgcgacctg ctaggtgtgg
      661 ttcgtgtcac ggcctctgcg gtcaatgtcg gagccggcac actgcaatcg gcacacggcc
      721 tcctgcccgc tccaggcccc gctgtggcga tactcgcgaa aggggtgccc atatacagtg
      781 ccggaccagc acgggaattg accacaccga ccggccttgc cttgttacga actctcacgg
      841 aggacttcgg gccgatgcct ccgatgaaac cggtcgccgt gggttatggc gcaggggatg
      901 ccaaccccga aggctggccg aatgtgcttc gggtgtttct gtcgcgagac gccgctactg
      961 gtggacgggg tcgcgacacc gtggtgcaga tcgaaacgaa cctggacgat gtcaatcctc
     1021 aaacctatga acacatcatg gagcgtctct tcggggctgg cgcccttgat gtggcgttga
     1081 ttcctgccat tatgaagcgt gggcgccccg cgatcgtgct gacctgcctt gcctccttca
     1141 ccgcggtcga ttctctgctg gatgtgctgt tcgaagagac cacggctttg ggagtgcgtg
     1201 tgcaggaaat cacccggcac atccttccac gacagtttac gtcggtgaag atccgcggtg
     1261 gcaccgtccg catcaagatc gcggcggtca atcagacgag cagcaaggcc gcgcccgagt
     1321 tcatcgattg taaacggata gccgaacgca cgggccgtcc cgtgaaggcg gtgttggaag
     1381 aggcgacatt ggcctatgcg aagacccgca gctcagcaaa ggaccgtctg tgacgctgtt
     1441 ggggtacgtc ctgctgttcg gagcctctgt cgtcattacc ctcggcgcct gcgcgctgtt
     1501 taccaacgcg attgaatggc tggggaagcg gttgaatctg tccgagggcg ccgtcggcag
     1561 cgtgctggcc gccatcggaa cgactctgcc cgaaacctcg atcccgatcg tcgcgatttt
     1621 tttcggcgcc actcgggagg aagcagaagt cggcctgggc gccatcctcg gcgcgccgtt
     1681 catgctcagt accttggtcg tccctctgct tgcagtgctc ctcttggtct atgcgcagtt
     1741 ggggaaacgc ggccgttcct tccgtctcaa ctacaaagac gtcacggtcg atctgtcctg
     1801 ttttgccctg gcctataccg tcgcgatggc ctgcaccttc gtgccgtcga ggacgttgcg
     1861 gattatcgtg gcggtggggt tgctgggttt atacagctat tacgtgaaaa tcaaattcag
     1921 cgacgatgat gccggcggtg agccgagcga tttgagtccg ctggtgtttg ccaaaaacag
     1981 tgcccggccg tcctatccct taatcggact gcaggcggtt gccggcctgg gagggctcgc
     2041 cctcggggcg catttgtttg tcatggcggc tgattcaacg gcgcacctgc tcgccatttc
     2101 tccgctgata ctagcgctgc tcgttgcgcc actcgcgacg gaactgccag agatgtcgaa
     2161 cagctttctt tggctctatc aaaaaaagga tcgtctggcc gtgggcaacg tcacgggcgc
     2221 aatggtattc caaggcaccg ttccggtttc ggtcgggttg gttggaaccg aatggacgct
     2281 cgcgccgtcg gcgctcctca cgatggtctt ggccgtcctg gcgacaggga gtttattgct
     2341 tcaagctgtc tggtctggtc actggcgacc gtggttgctc atcatcgggg cgatacttta
     2401 tataggatat gccttctatc tgtatggcta ttgatccgct gaactctgaa gacgagtgcc
     2461 gcgtgcgggg ggagcgtcct cacatcagca ccgacgtctt gacgtcttca ccgcgaaagc
     2521 gaccgtgaaa aaagctcaat cgccaacccg cgtccccatg ctcgggatta cgatgggtga
     2581 tcccgccggg attggaccgg aggtcattgc taaggccctc gcaggaaccg ccctccgtcg
     2641 gatctgtttt ccccttgtca tcggatcgca ggaggtcatg cagcgtaccg taaagcagtt
     2701 gaagctgaag ctcgccgtgc gtccggtgga gggccacgac cccattcgtc tgagcgcggg
     2761 acacgtcgct ctcctcgatc ctctcgagca gccgctcggt ccctacacgc ttggcgtggc
     2821 agcggctgaa accggcgcgg cttccgttgc attcatcaaa acagcagtgc gtctggccca
     2881 atcggaatgc attgacggga tggtcactgg gcccatcaac aaagaagcga tgaacatggc
     2941 cggctgccat tttcccggcc ataccgaact cctggcggac ctgacacaga cgccggaatc
     3001 cggcatgatg atcatcggcg gccggctcaa aataatgttt gtgacgacgc acattgctat
     3061 aaaggagcta cctgcggctt tgacccaagc caagatcgaa aagggcattc ggcttgcgca
     3121 tctcgctttg acgaccctct tcggcatcaa acaacctcgc atcggggtcg ccgcgctgaa
     3181 cccgcacgcc ggtgagcacg gactattcgg cgacgaagaa atccgcctga tcgggccggc
     3241 tgcacgcgcc gctcaagctc agggtattct ggcgagtgat ccactcccgg ccgatacttt
     3301 gtttggcaag accgccaggg gcgattatga cgccgtcgtg gcgctgtacc atgatcaggg
     3361 gttgattccg cttaagatgg tagcgtttgg aacctgcgtc aacctgacag tcggattgcc
     3421 gatcatccgc acgtcggttg atcatggcac cgccttcgac attgtcggga agggcattgc
     3481 cgatccgggg agtctcatcg aagcggttaa attggctgcg tcgcttgcac gacgtcgaac
     3541 gtctcacgct cgagcatagg agaacacgct caccatgcca ttggatgcca accatgcgct
     3601 gctcgtcata cagcagcaac tcaaggaact cgatgccatt tatcgggata ccctgcagat
     3661 gtcgctcaac accgtggccg ggaccgagcg agtcgcaaaa tggcgtgtcc gaacggtgtc
     3721 gttgctggcc gagtcggtgg gggaaaaggc agcgcaggat tttgctcggc tgcagcccgg
     3781 aatggtcttt acgaatgacc tcgtcgagga attcaccgat ctcgtggact gctttcgcgt
     3841 gcctctcagt gcgctggcca aacagctcac tcagacggcc cagcgtccgg ctgatggagg
     3901 ctcctaggtg cccggcccgg ctttcccgcg ggccagcaag cgactcgggc agcattttct
     3961 catcgaccag aatatcgtcc gaaagatcgt ggccgcggcg gaactgacgc cggatgatac
     4021 ggtgcttgaa gtcggacccg gccggggtat cctcacggag gcccttgctt cagtcgtccg
     4081 tcgtgtcgtg gccgttgaga tcgatccgca gctctaccgg tttctgcagg ctcggcagag
     4141 cgagtggaag aacgtcgaag tagtttgtgc cgatgcgctc tcgtattcgc tcgaagcagt
     4201 ggccgccggt gcagtcgtgg tggcgaacct cccgtactat atttccacgc ctctgctgtt
     4261 cagattcctc gagcatcgcc accgcttccc gcggctcgtg ctgatgcttc aaaatgaagt
     4321 tgccgaccgg ctggttgcaa aggccggcac atcggcatac ggcgtcttgt cggtgatggg
     4381 gcaatatgcg gccgacattt ctaaggcatt caccgtatcg gccaattgtt ttcgtcctcg
     4441 accggaggtg ggatccgcag ttgtcgtatt gcgcacgaag catgaagccc tcctctcacc
     4501 cgacgaagaa ccggcttttg ccgctgtggt gaaggcggcg tttgcgcata gacgaaagac
     4561 attggtgaac tccctccgcg atgagggata cgacacacag gcggtcattg atgccatcag
     4621 cgcagtcggg cttgtctcga ccgttcgggc agaaacactg agcctcgaca cattcagtcg
     4681 acttgcccga cggcttgatc gccgcaagcc gtcataaaca catgtcaata tgacagtcgt
     4741 cgtttgaatt ggttgacccc ctgtgttagc atccggcaca tgggcgtaac cagctcggcc
     4801 aagcggggcg aggcccgccg cgcccctgct cccccttccc atctcaagcg tgaagtgatc
     4861 ggcgtcatcc tgattgcgct gagcctgctc acgctattaa gcctcctgtc ctttgtgccg
     4921 ggtgagccga aggtcgtcgc taccccagcg gtgacgatga cccccccgac tcacaacctg
     4981 atcggctccg tgggtgcggt cttttcctcg ctgttgtttt ctcttatcgg cggcgccgcc
     5041 tatctgtttc ccatcctgtt gggactgctc ggcgggcgat gtttcactca gagcgatctt
     5101 tcaatcaggc tacgcaacgc aggggcatcg ctggccgctc tgttgttcct gagcggcctg
     5161 cttcacttgg aagtgattgc ggtgccgacc gtttcgagcg gaatggtctt tcgaggccag
     5221 gcaggagggc tcctcggaca actgctggcc gaaggattgc gtgcctattt tgcgaatacc
     5281 ggcacccata ttctcatcat ctccggcttt ctcgtggcgc tgctcttcac cgcgcccttg
     5341 tcgctggctc aacttgctgt gcaagcgcca cagtggggcc gaaacgcatt tgcacgactg
     5401 aacgctctga tgccgaggcg ggagcaagcc gcggcgacgc cggatccgcc gaagccgacc
     5461 aagacgcgaa aggcaaagtc aattcgtgcc gtcatagaag aagtcctccc gccggccgaa
     5521 gacaaccctc ccgccgaagt gttttctgtg acgccgccaa cggtcgtgtt gccagagccg
     5581 cgtgaaattg ttgaactgga gccggatgcc ccggtcgtcg gtaaagtcga ggacaccggc
     5641 gactatcagt tgccggatgc ggatgaatta ttaacggaac cctcaggaat actcgcccgc
     5701 atcaccgacg aggaactgaa agggcagtcg gatattctgt cgaaagcgtt gctgagtttc
     5761 ggcattgaag gcagagtcac cgaggtgcgg ccaggcccgg tcgtgacgat gtatgagttt
     5821 gagccggcac c
//
LOCUS       DASTQY010000163        16230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_222696, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000163 DASTQY010000000
VERSION     DASTQY010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 16230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16230
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222696"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VFR82_05275"
     CDS             complement(<1..604)
                     /locus_tag="VFR82_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4501043.1"
                     /translation="MGDPKGFMKYAREGPKRKPVELRVLDWKEMYEPISEDRLKIQGA
                     RCMDCGVPFCQGNTGCPVVNLIPEWNDLVYRGRWQDALKALHTTNNFPEFTGRLCPAP
                     CEGACVLGINEEPVSIRVIEWNIIDRGFNEGLVFPVLPVARTGKTVAIVGSGPAGLAA
                     AQQLARAGHSVTLFEKADRIGGLLRYGIPDFKMEKWVIDRR"
     gene            complement(732..5249)
                     /gene="gltB"
                     /locus_tag="VFR82_05280"
     CDS             complement(732..5249)
                     /gene="gltB"
                     /locus_tag="VFR82_05280"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEU4501044.1"
                     /translation="MEFPGLPPKQGLYDPRHEKDSCGIGFVVNIKGHKSHDIIRQGLQ
                     VLENLTHRGAQGCDPCTGDGAGILLQVPHQFLRRAAGDVGVSLPGAGEYGVGQVFLPP
                     HAAARQQCETLFGTIIEKEGSRVLGWRDVPVKSDTIGVQARKTEPYMRQVFIARDMLN
                     EAQFERKLYVIRKLVEKAVRESAIQGREHFYIPSLSASTIVYKGLLLPHQMGVYYQDL
                     TDASMVSALALVHSRFSTNTFPTWPLAHPYRHICHNGEINTLKGNVNWMKARQGRLHS
                     DLFGKDMEKVFPIVYEQQSDSACLDNALEFLLLGGRSLPHAMMMLIPEPWVANAQMDL
                     DRRGFYQYHAAMMEPWDGPAAVCFTDGRMIGATLDRNGLRPCRYQVTTDGTVVLASEA
                     GVLPVEPKQIRMKGRLQPGRMFLVDTVQGRIIDDEEIKADIVGRKPYRSWVTQYGVSL
                     DELPEPLNVPQPDHPTIRQRQQAFGYTVEELKMVITPMIVTGEEAISSMGTDTPLAVL
                     SDRPQLLFKYFKQLFAQVTNPPIDPIREQLVMSLVTNIGPKPNLMDESPESCRRIKVQ
                     QPILTNADLQKIRGISDPHFKSKTLRMLFRVAEGPDGLGAAVDAMCRDASQAIKDGYK
                     FLILSDRGVDEEWAPMPSLLGISAVHHHLVRECTRTEVGLILETGEPRDVHQFACLIG
                     YGAGTINPYLVFESLVDMERDNYLPEGLDAQTAEGKFIKAINKGLLKIFSKMGISTVQ
                     SYCGAQIFEAIGLNHEIIDRYFTGTPSRVEGVGIREIGEETLRRHRVAYEPAAIRQLD
                     FGGEIHYRIQGEHHNWNPDTIYKLQHASKSNDPKTYTEFAQLVNDESTRRSNLRGLLD
                     FKFDQTPLPVEEVEPASEIVKRFNTGAMSFGSISKEAHETLAIAMNRLGGKSNTGEGG
                     EDPERFKPLPNGDSKNSYIKQVASARFGVTAHYLVNARELQIKMAQGAKPGEGGQLPG
                     HKVDENIAKFRYATPGVQLISPPPHHDIYSIEDLAQLIFDLKNSNPDAAVSVKLVAEV
                     GVGTVAAGVSKAHADKVLISGDSGGTGASPLSSIKYGGIPWELGLAETHQTLVLNDLR
                     GRIRVETDGQMKTGRDVVIATLLGAEEYGFATAPLIVEGCVMMRKCHLNTCPVGIATQ
                     DPELRKKFNGKPEHIVNYLFFVAEEARKLMAKLGFRTINEMVGRVDKLKITKAVNHWK
                     AKGLDLTPLLTMPDVAPEVARYCVQKQDHGLDCILDNTLVDLCKPAIERGERIQLDLP
                     IKNVNRTTGTVLSSKIAKKYGLEGLPEDTISIKFTGSAGQSFGAFLSRGVTLTLEGES
                     NDYIGKGLSGGKIIVYPPKNVLYNPEDTILIGNTSLYGATQGEAYFYGMAGERFAVRN
                     SGARAVVEGTGDHGCEYMTGGVVVVLGRTGRNFAAGMSGGIAFVLDDVGRFQSRCNTG
                     MVELEPVSTKEDKQQLHDLITKHFMYTGSRKAKQVLDAFDATLPKFVKIMPVDYKRVL
                     EERKRKASAVH"
     gene            5470..6474
                     /locus_tag="VFR82_05285"
     CDS             5470..6474
                     /locus_tag="VFR82_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU4501045.1"
                     /translation="MPQGPVTSSIHTMADVWDAYRSELDGVEDRIHQNLTSSVTLVNT
                     VAAHILNSGGKRIRPLLLLLCARLCGYTGREHHQLGSLVEFIHTATLLHDDVVDDADI
                     RRGRQTARKVWGNQVSILVGDYLYSKAMCQVVEFRSQKINEVLAEACKKMAEGEVLQL
                     YYNGNPSMPEGDYIKIVEHKTAGLIAASCRMGAILADASEAQQDCVFRFGQYLGIAFQ
                     VVDDTLDYTANGASLGKTLGQDLRQGKATLPLLHLLNHCSDRDRQMIKDRMETRTLTE
                     GDLQRLIDLMDESGSIVYALHHAQAYITAAKRELGQFEESTAKRALSVTADYMVTRDR
                     "
     gene            6623..7942
                     /locus_tag="VFR82_05290"
     CDS             6623..7942
                     /locus_tag="VFR82_05290"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEU4501046.1"
                     /translation="MAQIIPFRGTLYDLSSIGSIRNVVAPPYDIIDAAGQKALHDRHP
                     QNIIRLELGIDEPGDNATNNRYMRAAETLRAWIAHGALKRDAQPAIYYHTIEYVPPSS
                     DARAPKKTLRGFLTTTKLEPLDSGHIYPHENTRSAAKTDRLNLLKSCKANLSPIWLLY
                     SDPHSTILGLLEKAVKGQPARIDFEDDSGCRQRLWAVTDPSILQQIVDTMASKPLFIA
                     DGHHRYETALNYQRFRRETDGPSGDLKPYDSVLMLLSPLEDPGLTVLPTHRVITTPLP
                     SHGQVTARLADSFDLTECSFTATTKAAVREQFLEALRINGRTVPTFGLALKGDDRYLM
                     LSLKAAHRPPADASPRTKLDVSLLQHSIVSVLCPTQQEQEAILYTKDDHEALDWAAEG
                     PGTAALLLNATKVSEVQAVAIAGERMPHKSTYFYPKPLTGLVIHVME"
     gene            7994..9409
                     /locus_tag="VFR82_05295"
     CDS             7994..9409
                     /locus_tag="VFR82_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU4501047.1"
                     /translation="MKAKILIVDDDRDIATMLEDRLQASDYGTILAEDGVQALEKVEQ
                     EAPSLMLLDLDMPRLGGLDVLKRLPTIKQAEDIPVIVMTAHGSIEAAVEAMKHGAYDF
                     LTKPLDKDHLLIVIGKALERASLKRHVACLKSEVDSRYASIIGTSTKIRAVLESAQRA
                     AKSDAGVLLLGESGTGKELLARSIHQWSPRHAMPLVVINCVALTETLLENELFGHERG
                     AFTGADRLQKGKLEMADGGTVFLDEIGDMSLPLQSKLLRVLQDREFHRVGGTRLVSVN
                     IRIIAATNKDLKQAVKSGDFREDLFFRLNVIGLTLPPLRERLEDFPALAKFFLSRHAK
                     EAKRPGMWFSPAALDALSRYSWPGNIRELDNVIARAVILSHTDLIEPEVLALDFNSPV
                     VKEDVVPYLRMPYHESMDAHSRHIIEQALHDADGNQTKAAERLKLQRTYLARLLKQQR
                     LKLEARGDETDEDVTAQTAQL"
     gene            complement(9551..10291)
                     /locus_tag="VFR82_05300"
     CDS             complement(9551..10291)
                     /locus_tag="VFR82_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEU4501048.1"
                     /translation="MILAAGLGTRLRPLTNTIPKPLLPIAGTPLIVWNLLLLKRHGIN
                     DVVINLHHLGPMIEQALGNGSKYGLKIYYSHEPVILGTGGGIKQAECHFSGEPMLVLN
                     GDTLFELDLDALYAFHHRRQASATLVLRSDADAARWGLVEVDMEDRIVRITGRGKTDV
                     GATKPRMFAGVHILSPRLLRDVPKGRASSIIDAYVAAIQRGEAVAGYELMGYWSDVGT
                     PERYAQAEHDATSGLIRLSERLPAAPLA"
     gene            complement(10382..11482)
                     /locus_tag="VFR82_05305"
     CDS             complement(10382..11482)
                     /locus_tag="VFR82_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU4501049.1"
                     /translation="MATIDPATTKKSLAPPDRALIAETIRTRLPVRATFKKLSPLAGD
                     ASNRRYFRLELGDAAQSVILMQLAEPEAFKQSEEAVSGAGHQIAELPFVNIQAHLAKA
                     GVPVPDLYYYDQTAGLLYLEDFGDLTLSEACAHAGLPQLENRYRQAIDVLVRMQVKAT
                     SPANPQCLAFHRSFDVPLLMWEFDHFLEYGIAAKQGAPMHDGDAAAIRQEFRKISELL
                     AAQPRVFVHRDYHSRNLMVDEERLGVIDFQDALMGPATYDLASLLRDAYIALDESLID
                     RLVAYYLDQLQESRQLWTNREAFRRLFDFTCIQRNLKAAGRFVYIDRVKGNPKFLADI
                     PRTLGYVKRNLHKYPELFTLRKLMTLYVPELQ"
     gene            complement(11517..11948)
                     /locus_tag="VFR82_05310"
     CDS             complement(11517..11948)
                     /locus_tag="VFR82_05310"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU4501050.1"
                     /translation="MQKTYAESIQSPAAQWLRYWFPVIAYVTLIFYLSSLSHPEERLP
                     NFLFEQLGDKLLHGIEYAVLALLCYRAFRWAAGPHAGEYAVGLAIVASSLYGLTDELH
                     QAYVPLRESSVVDWIADTTGAVIGAVGSRRLMERGTKPSIS"
     gene            12028..14226
                     /locus_tag="VFR82_05315"
     CDS             12028..14226
                     /locus_tag="VFR82_05315"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HEU4501051.1"
                     /translation="MKTVRVCFLWHMHQPYYTDPVSGSVSMPWVRLHAAKAYFDMAYL
                     IDQFPRVHATFNFTPSLLVQLQEIGDGSVQDLFWERTQRQAARLNEEEQAFLIKHFFS
                     INWTTMIRPYPRYHELLTKRGFDIDGQELHRLARQFTTQDFLDLQVWFNLAWFGYGAV
                     ARYPRLTALRSKNRGFTEEEKQEVLALQRRTVQDIVPMYRRLAERGQIELTTTPFYHP
                     ILPLVIDTEHTRRARPDLPLPSRLRAPDDAEAQLRLAVEYHLSVFGRPPAGLWPSEGS
                     VCPELIPLLRRVGLRWLATDEGILTRSLEASEVPWHRETQLYRPYRVGPTGEEATIIF
                     RDRDLSDGFGFLYHKTTPHAAADDVLRRMKRIAVDTPQDNVLLPIILDGENPWEHYQE
                     GGEQFLSHLYQALSDGALNEGDSICVTTATVSNALEATPDRPYLAHLHSGSWINQDFK
                     IWIGHAEDNRGWDLLNHTRSRLLEISSTLPSNRARAAWQELYAAEGSDWFWWYGDDFD
                     TTYKEEFDWLFRMHLRNVWTLAGMTPPDLLNQPLCEIRRFEGRDQVTMPLAILHPRLD
                     GLVTDFFEWRGAGTINPNPPLGAMWKAEGLFTAIFFVCDFEHLYLRLDPDEAFKSRKS
                     DLRLECVLQAPRGAYRLIISSASPDRYRLAQRRDDGNWGDIGSCELIGWNQVLELAVP
                     FKEIQVGESERVQLSLLVYEHELEIARYPRHQPAIVTVPGPEFGATVWRV"
     gene            14254..15258
                     /gene="galT"
                     /locus_tag="VFR82_05320"
     CDS             14254..15258
                     /gene="galT"
                     /locus_tag="VFR82_05320"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEU4501052.1"
                     /translation="MPDLRRDPIVGRWVIISTDRNGRPHDFAQMQPVRPISTALCPFC
                     PGQERLTPKEIMAYRPEPAEANAPNWSVRVVPNKFPALQVEGDLGREGMGLYDRMNGI
                     GAHEVIIEAPGHKDSLADMPTNRLEDVLWAYRDRIIDLKRDVRLRYILIFKNHGAAAG
                     ATLEHSHSQLIALPVIPTSVMNEIEGCRLHYQQKERCMYCDILKQDLAQGERIVAENA
                     EYACVTPFAPRFPFEMWILPKRHAGHFEQSEKKHFEFLAPILSESLRRMDRVLSKPAY
                     NFVLHSSPVHETTGEFYHWHIELIPKLTQVAGFEWGSGFYINPVSPEESARYLREAVL
                     "
     gene            15306..15506
                     /locus_tag="VFR82_05325"
     CDS             15306..15506
                     /locus_tag="VFR82_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU4501053.1"
                     /translation="MHVQLSHPARTVEIKGPKRTKDLLRELNLVVEAHLVIRGDELVT
                     EDEMLSDKDQIEIRPVISGGTP"
     gene            complement(15556..>16230)
                     /locus_tag="VFR82_05330"
     CDS             complement(15556..>16230)
                     /locus_tag="VFR82_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEU4501054.1"
                     /translation="PKNVMNASALSSVRCLRIFHLNKCARHLHENTVMTRRLLISGVL
                     LVVAGCTTLPKTGYDFDRSTNFGGYKTYAWVSGEQEKTGDRRADNSAVDMRIRIAIGT
                     QLRLKGYQPLPEGRPDFLVTYTIALKDSSPTVSSQYFSEGMAGRPFLHSADSRSVRDT
                     TPSNSEGHSYLTGTLLIDVIDGASQKLVWRGTAAGEVDQGLTSEQRDERTKAIVRNIL
                     SHFPPQ"
BASE COUNT         3380 a         4830 c         4559 g         3461 t
ORIGIN      
        1 ggcggcggtc gatcacccac ttttccattt tgaaatcggg aatgccgtag cgaagcaagc
       61 cgccgatgcg gtctgctttt tcaaagagcg tcacactgtg gcctgcgcgc gcaagttgct
      121 gcgccgccgc gagcccggcc ggcccggatc cgacgattgc cacggttttt cctgtcctgg
      181 ccactggcag aacgggaaat accaggcctt cattgaaccc tcgatcgatg atgttccatt
      241 cgatcacgcg gatggatacc ggttcttcgt tgatgccaag cacgcaggcg ccttcacacg
      301 gagccggaca gaggcggccg gtgaattccg gaaagttatt ggttgtgtgc agggccttga
      361 gggcatcttg ccaccgcccc cgatagacga gatcgttcca ttcaggaatg aggttgacga
      421 cggggcagcc ggtgtttccc tgacaaaacg gcacgccgca atccatgcag cgcgcccctt
      481 gaattttgag tctatcctcg gaaatgggct cgtacatctc cttccaatcg agcacccgga
      541 gctcgaccgg tttccgtttc gggccctccc gggcgtattt catgaaacct tttgggtcac
      601 ccatggcttt tcaatgtttg tctttcaatt gtttcaatat gacctggagt cgaagaccct
      661 caatcttact cactcatccg ccgtgcgccc gcgggggcgt gcgcttgacc caagttggat
      721 cacccatggt tctagtgtac cgcgctggct ttgcgtttac gttcttcgag gacgcgttta
      781 tagtcgaccg gcatgatctt gacgaatttc ggcagcgtgg catcgaatgc atcgagcacc
      841 tgttttgcct ttcggctgcc cgtatacatg aaatgctttg taatcagatc gtgcagctgc
      901 tgcttgtctt ccttcgttga gaccggctcg agctcgacca tgcctgtgtt gcaccggctc
      961 tggaatctcc cgacatcgtc cagcacgaag gcaatgcccc cggacatgcc tgccgcaaaa
     1021 ttgcgaccgg ttcgaccgag caccaccacc accccaccgg tcatgtattc gcaaccgtga
     1081 tcacccgtgc cttcgacgac cgcccgtgcc ccgctgtttc gcacagcaaa ccgctccccc
     1141 gccattccat agaagtaggc ctcaccttgc gtcgctccat acagcgatgt attcccgatg
     1201 aggatcgtgt cctccggatt gtacaaaacg tttttcggtg gatagacgat gattttgccg
     1261 ccggacaggc ccttaccgat atagtcgttg gattcacctt ccaaggtgag cgtcacaccc
     1321 ctggaaagaa aggccccgaa cgattgcccg gccgaccccg tgaacttaat cgagatggtg
     1381 tcctccggca atccttccaa cccgtacttc tttgcgatct tgctggaaag caccgttccg
     1441 gtggtgcggt tcacattctt gatcggcagg tcgagctgga tcctctcccc tctctcaatg
     1501 gccggtttgc aaaggtccac gagcgtgtta tcgagaatgc agtccagccc atggtcctgt
     1561 ttctgcacgc aataccgcgc cacttccggt gccacatccg gcatggtcaa cagcggcgtc
     1621 aagtccagtc ccttcgcctt ccagtgattg acggcctttg tgatcttgag tttgtccaca
     1681 cgaccgacca tctcgttgat cgtgcggaac cccagcttgg ccatcaactt ccgcgcttct
     1741 tcggcgacaa agaataaata gttcacaata tgttcgggct tcccgttgaa ctttttccgc
     1801 agctccggat cctgggtggc gatcccgact gggcaggtgt tcaaatggca cttgcgcatc
     1861 atgacgcagc cttcgacgat caacggcgct gtcgcaaagc cgtactcttc ggcgccgagc
     1921 aaggtcgcga tgacgacatc tcgtccggtt ttcatctggc cgtccgtttc gacgcggatg
     1981 cgcccgcgca aatcgttgag gacaagagtc tggtgcgttt ccgcaagacc cagctcccag
     2041 ggaatgccgc catacttgat cgaagagagc ggcgaggcac cggttccgcc ggaatcaccg
     2101 ctgatcaata ccttatccgc gtgggccttc gatacgcccg cggcgacggt accgacaccg
     2161 acttccgcaa ccagttttac agatacggct gcgtccggat tgctgttctt gaggtcaaaa
     2221 atgagctgcg ccaggtcttc gattgaatag atgtcatggt gcggcggcgg cgaaatgagc
     2281 tgtacgccgg gcgtcgcata acggaacttc gcgatgtttt cgtccacttt gtggcccggc
     2341 agctggccac cctcacccgg cttggcgcct tgcgccattt tgatctgcaa ttcgcgagca
     2401 ttcaccaagt aatgagcggt caccccgaag cgggccgagg cgacctgctt gatatagctg
     2461 ttcttggaat cgccattcgg caaaggttta aaccgttcag gatcttcccc gccttcgccg
     2521 gtattgctct tcccgcccag cctgttcatc gcgatcgcca acgtctcatg cgcttctttg
     2581 ctgatcgacc cgaacgacat cgcacccgtg ttaaaccgct tcacaatttc actcgcgggc
     2641 tccacttctt cgacgggtag cggggtctga tcaaatttga aatcgaggag gccgcgcagg
     2701 ttggaccggc gcgtgctttc gtcgttcaca agctgcgcaa actcggtata ggtctttgga
     2761 tcattcgact tgctggcgtg ctgtagcttg taaatggtgt cggggttcca gttatggtgc
     2821 tctccctgga tccgataatg tatctcgccg ccgaaatcga gctgtcgaat ggcggcgggc
     2881 tcgtacgcga cacgatgccg ccgcagggtc tcttcgccaa tctcccggat ccccacgcct
     2941 tccacccgtg acggcgtgcc ggtgaaatag cggtcgataa tctcgtggtt caatccgatt
     3001 gcctcaaaaa tttgcgcacc gcaatacgac tgtacggtgg agatacccat tttcgagaag
     3061 atcttgagga ggcctttatt gatcgccttg atgaattttc cttccgccgt ctgcgcatca
     3121 aggccttccg gcagatagtt atcgcgctcc atgtcgacga gcgattcgaa cacgagatag
     3181 gggttgatgg ttccggcgcc gtagccgatc aggcatgcga actgatggac gtcccgcggc
     3241 tcgcccgttt cgagaatcag tcccacctcg gttcgggtac actcgcgcac gagatggtga
     3301 tgaacggctg agatgcccag cagactcggc atcggcgccc attcttcgtc gaccccccgg
     3361 tcactcagga tcaggaactt gtagccgtcc ttgatcgctt gagatgcgtc tctgcacatg
     3421 gcatccaccg cggcgcccaa tccgtcgggt ccttcggcca cacggaagag catgcgcagc
     3481 gtcttgctct tgaaatgcgg atcggagata ccgcggattt tttgcaggtc ggcattggtg
     3541 agtatcggct gttgcacctt gatccggcgg caggactccg gcgactcgtc catcaaattg
     3601 ggcttcggac cgatgttcgt cacgagcgac atcacgagct gctcgcggat cggatcgatc
     3661 ggcgggtttg tcacctgggc gaaaagctgc ttaaagtatt tgaacagcag ttgcgggcgg
     3721 tccgacaaca cggccaaggg cgtatccgtc cccatcgatg agatggcttc ttcgccggtc
     3781 acgatcatcg gcgtaatgac cattttgagc tcttccaccg tgtacccgaa tgcctgctgc
     3841 cgctggcgga tggtcggatg gtccggctgc ggaacgttca acggttccgg tagttcgtcc
     3901 agtgagacgc cgtactgcgt gacccaactg cgatacggct tgcggccgac gatgtcggct
     3961 ttgatctcct cgtcatcgat aatgcggccc tgcacggtgt cgaccaggaa catgcgcccg
     4021 ggctgcaacc gccccttcat gcggatctgc ttcggctcga cgggcaagac gcccgcttcc
     4081 gacgccagca cgaccgtgcc gtccgtggtg acctgatagc ggcagggccg gagaccgttg
     4141 cggtccagtg tcgccccgat catcctgccg tccgtgaaac agaccgcggc cggaccgtcc
     4201 cagggctcca tcatggcggc gtgatattga taaaacccgc ggcgatccag atccatctgt
     4261 gcattggcga cccacggctc gggaatcagc atcatcatcg cgtgtggcaa cgaccggccc
     4321 cccagcagta aaaactcgag cgcattatcg agacatgccg agtcgctttg ctgctcgtac
     4381 acgatgggga acaccttttc catgtccttg ccgaacagat ctgaatggag ccgtccctgc
     4441 cgggccttca tccaattcac attgcccttg agggtgttga tttcgccgtt gtggcagatg
     4501 tgacgatagg gatgggcgag cggccaagtc ggaaacgtgt tggtactgaa gcgcgaatgt
     4561 acgagcgcca gcgcgctgac catgctggca tctgtgaggt cctgatagta cacccccatt
     4621 tgatgcggca gcagcaaccc tttgtagacg atcgtgctcg cggacagact ggggatgtag
     4681 aaatgttcgc ggccctgaat ggccgattcg cgcacggctt tttcaaccag cttgcggatc
     4741 acgtagagtt ttcgctcaaa ttgcgcctcg ttcagcatgt cgcgagcaat gaacacttgg
     4801 cgcatgtagg gctcagtttt acgggcctgc acgccgatcg tatcgctttt gaccggcacg
     4861 tcacgccatc cgagcacccg tgagccttct ttctcgatga tggtgccgaa caaggtttca
     4921 cattgctgcc gggcagcggc atggggaggt agaaacacct ggcccacgcc atattcaccg
     4981 gctcccggaa ggctcacgcc gacgtcaccc gccgcacgcc gcaggaattg atgaggaact
     5041 tgtaaaagta ttcccgcgcc gtcaccagta cagggatcac atccctgcgc gccacggtga
     5101 gtgagatttt cgagcacttg gagaccctgg cggatgatgt cgtgcgattt gtggcccttg
     5161 atgttgacga caaacccaat cccgcacgaa tccttctcgt gtcgagggtc gtagagccct
     5221 tgcttcggtg gaagccctgg aaattccatt cttacctatc cagtgaaaaa ggcaggtctt
     5281 tttcctgtcg agtagaggcc ctatcgagag gaatcagaga tgcggaaacc ccttgctcgc
     5341 gaggcatgtc ttcgctcgaa ttcgtgggtc agattaatgg aaggctgatt cttctgtcaa
     5401 gccgacaatg cacggatttc tccgcgattc ccttgacttt gtttcatctg ctgccctacc
     5461 atccgccgca tgccacaggg tcctgtcacc tcctcgattc acacgatggc ggacgtatgg
     5521 gatgcctacc gtagtgagct ggacggtgtc gaagatcgca tacaccagaa tctgacttcc
     5581 agcgtcaccc tcgtcaatac ggtcgctgcg catattctca acagcggggg caagcgcatc
     5641 cggccactct tgcttttgct ctgcgcgcgc ctctgcggct ataccggccg cgagcatcat
     5701 caactcggta gcctcgtcga gttcatccac acggccaccc tgctgcatga tgacgtcgtc
     5761 gatgatgccg acatcagacg gggccgtcaa accgctcgaa aagtttgggg aaatcaggtc
     5821 agtatccttg tgggcgacta cctctattcc aaggccatgt gccaggtcgt cgagtttcgc
     5881 agccaaaaga tcaacgaagt gcttgccgag gcctgcaaga aaatggctga aggggaagtg
     5941 ctccagctgt actacaatgg caacccctcg atgccggagg gcgactacat caagatcgtg
     6001 gagcataaga ccgcgggact gatcgccgcc tcatgccgga tgggtgcgat tttggccgat
     6061 gcgtccgagg cacagcagga ctgtgtattc cgcttcggac aatatctcgg catcgcgttt
     6121 caagtcgtcg atgacacatt ggactatacg gcgaacggcg cctctctagg gaagacgctc
     6181 ggccaggatc tccggcaggg caaggcgacg ctccccctcc tccatttact gaaccattgc
     6241 tccgacaggg atcggcagat gatcaaagac cggatggaaa ctcgcacgtt gacggaaggc
     6301 gatttacagc gactgatcga cctgatggac gagtctggat ccatcgtcta cgccttgcat
     6361 catgctcaag catacatcac cgccgcgaag cgagagttgg gacagttcga agagagcacc
     6421 gctaaacggg ctctctcggt aaccgcagat tacatggtga cccgcgaccg gtagccgctc
     6481 cgcgtggtct caagccattc ggcatgagac gggcaacgcg cggaactggc ggcccagcct
     6541 ttgaaggaaa cccgcaggat ggtcaaggac cgtcccgcgc ggccgcagca aacgactttc
     6601 aacatcctgc agagaggagc gcatggcaca gatcattcca tttcgcggca cgttgtacga
     6661 cctatcgtcg attgggtcca tccgcaacgt cgtggctcca ccctatgaca tcatcgatgc
     6721 cgcagggcaa aaagctttgc acgaccggca tccgcaaaat attatccgcc tcgagctcgg
     6781 cattgatgag cctggcgaca atgccacgaa caaccggtat atgcgcgctg ccgagacatt
     6841 gcgggcctgg atcgcccatg gcgcgcttaa gcgggacgcc caaccggcca tctactatca
     6901 caccatcgag tacgtgccac cgtcgtcgga tgcgcgggcg ccgaagaaaa cgctgcgggg
     6961 gtttctgacg acgacgaaat tggagccgct ggactctggt cacatctacc cccatgaaaa
     7021 cacgaggtcg gctgcaaaga ccgaccggct caatctgctc aagtcatgca aggcgaatct
     7081 cagtccgatt tggctgctct attcggatcc ccacagtacg atcttgggcc tgcttgaaaa
     7141 ggccgtgaag ggccagcccg ctcgaatcga tttcgaggac gacagtggct gtcggcagcg
     7201 gctgtgggcg gttaccgatc catccatcct tcagcaaatc gtggacacca tggccagcaa
     7261 gccgctcttc attgccgacg gccatcatcg gtatgagacc gcactcaact atcagcgctt
     7321 ccggcgggag acagacgggc cttccggcga tctgaagccc tatgacagcg tgctcatgct
     7381 tttgtccccc ctcgaagacc cgggtttgac cgtgcttccc actcatcgtg tgatcacgac
     7441 cccgctaccg tctcatgggc aggtcaccgc ccgtttggct gactccttcg acctgacgga
     7501 gtgttcgttt acggcgacga caaaggccgc agtgcgagaa caattcctgg aagccttgcg
     7561 catcaacgga cgaaccgttc cgaccttcgg tctcgcactc aagggagatg atcgctatct
     7621 gatgctctcg ctcaaggcgg cgcaccgtcc tcccgcggat gcttccccgc gtacgaagct
     7681 agatgtctca ctgcttcaac actcgatcgt gagcgtgctg tgcccgacac agcaagagca
     7741 ggaagccatc ctatacacca aggacgatca tgaagcgctg gattgggcag ctgaaggacc
     7801 agggacggcc gccctgctcc tcaatgcgac caaagtgagc gaagtgcagg cggtggcgat
     7861 tgcaggcgaa cgaatgccgc acaagtcaac gtacttctat ccgaagccgc tgacggggct
     7921 ggtcatccac gtcatggaat gatgaccgga gtgtctgaca tcaactgggt gataggcgat
     7981 ggagggcaaa cacgtgaaag ctaaaattct gatcgtcgac gacgatcggg acattgcgac
     8041 gatgctggaa gaccgccttc aggcctccga ctatggcacg atcctcgccg aagacggcgt
     8101 ccaggccctg gagaaagtgg agcaggaggc acctagcctc atgctcctgg acctcgacat
     8161 gccccgactc ggcggtctcg acgtcctgaa acgcctaccg acgatcaagc aagccgagga
     8221 catcccggtc atcgtcatga ccgcccacgg ttcaattgaa gcggcggtcg aggcgatgaa
     8281 gcatggggca tatgatttcc tcacgaagcc cctcgacaag gatcatctgc tgattgtgat
     8341 cggcaaggcg ctggagcgtg cgtcattgaa acggcatgtc gcctgcctca agtcagaggt
     8401 ggacagtcgc tacgcatcga tcatcggcac cagtaccaag atcagggccg tcctggaatc
     8461 tgctcagcgc gccgctaagt cggacgccgg tgtgctcctg ttgggagaga gcggcacggg
     8521 caaggaactg ctcgcgcggt ccattcatca gtggagtccc cgccacgcca tgccgctggt
     8581 ggtcatcaat tgcgtcgcct tgaccgagac cttattagaa aacgaattgt tcggtcatga
     8641 gcgcggggcc ttcaccggcg ccgatcgtct gcaaaagggc aagcttgaaa tggccgatgg
     8701 cggcaccgtg tttctcgacg aaatcggaga tatgtcgctc ccgctgcaat ccaagctctt
     8761 gcgcgtgctt caagatcggg aatttcatcg tgtcggcggg accagactcg tgtcggtgaa
     8821 catccggatc attgctgcca ccaacaagga tctgaagcag gccgtcaaga gcggagactt
     8881 ccgcgaagat ctgtttttcc gtctgaatgt gatcggcctg actcttccgc cgctgcgcga
     8941 acgattggag gactttcccg ctctggcaaa atttttcttg agccgccatg ccaaagaagc
     9001 caagcgccca gggatgtggt tcagtcccgc tgccctggac gccttgtccc gctattcctg
     9061 gccgggcaac attcgagaat tggacaatgt catcgcccgg gccgtcattc tgagccacac
     9121 cgatctcatc gaacccgaag tgttggcgct cgatttcaat tcaccggtcg tgaaagagga
     9181 tgtcgtgccg tatctcagga tgccctacca cgaatcgatg gacgcacaca gccggcacat
     9241 cattgagcag gcattacacg atgctgatgg gaaccagacg aaagccgccg agcgcctcaa
     9301 gctccaacgc acctacctgg ctcgactgct caaacagcag cggctgaaac tcgaagcaag
     9361 aggcgatgag acggacgagg acgtaaccgc acagaccgca caactgtgaa gaggaagacc
     9421 cgtttcaagg ctggtcaatc agccgtcgtt cccacccccc agccccagca tgcccagctc
     9481 gcccacgacg cggacggagc cgcgagttct gcgatacgta gaacgaccat aatcctctct
     9541 tcaacagctg ttaagccaac ggagcggcgg gtaaccgttc actgagccga atcagaccgc
     9601 tggtggcatc gtgctctgcc tgtgcatagc gctcgggcgt ccctacatcc gaccaatagc
     9661 ccattagctc atacccggca accgcttctc ctcgctgaat cgccgcgaca tacgcatcga
     9721 tgatcgaaga cgctctgccc ttgggcacgt cccgcaacag acgcgggctg agtatgtgaa
     9781 cgccggcgaa catgcgcggc tttgtcgcac cgacatccgt cttgccgcga cccgtgattc
     9841 gaacgatacg gtcttccatg tccacctcga ccaatcccca gcgcgctgca tcggcatccg
     9901 accgcaggac caaggttgcc gacgcctgac gccgatgatg aaaggcatag agcgcatcga
     9961 ggtccagctc aaacagggta tcgccgttca ggactagcat cggctcgcct gagaaatggc
    10021 attccgcctg tttgatgccg ccgccggttc cgagaatcac cggttcatgc gagtaataga
    10081 tcttgagccc gtacttggat ccgttcccta acgcctgctc gatcatcggg cccaaatgat
    10141 gcaagttgat gacgacgtca ttgatgccat ggcgtttcag aagcagcaga ttccacacga
    10201 tcaagggtgt tcccgcgatg gggagcagtg gtttgggaat cgtattggtg agcggccgaa
    10261 gccgtgtgcc caagccggcg gcgaggatca tagctttcac ttatgccagt gctgagccgg
    10321 tcgtgccgaa cactgagcga ccggatggcc gccagatttc agatcccggt actcagtccc
    10381 cttactgcag ttccggcaca tacagagtca tcagtttgcg cagcgtgaac aactcaggat
    10441 atttgtggag attgcgtttg acatatccga gcgtgcgagg aatatccgcc aggaatttcg
    10501 ggttgccctt gacccgatct atatagacaa accgtccggc cgccttgaga ttgcgctgaa
    10561 tgcacgtgaa gtcgaacaga cggcggaacg cttcccgatt ggtccacagc tgacgactct
    10621 cctggagttg atcgaggtaa taggccacaa gccggtcaat gagtgactcg tccaatgcga
    10681 tgtacgcgtc gcgcagcagg gaggccagat cgtaggtcgc agggcccatc agtgcgtcct
    10741 gaaaatcaat cacgcctaat cgctcttcgt ccaccatcaa atttctggaa tggtaatcgc
    10801 gatgcacaaa gacccgcggt tgcgccgcga gcagttcgga aatcttgcgg aactcttggc
    10861 ggatggcggc cgcatcaccg tcatgcatcg gcgctccctg ctttgccgcg atcccgtatt
    10921 cgaggaagtg atcgaattcc cacatcaaca gcggcacgtc aaagcttcga tggaatgcga
    10981 ggcactgagg gtttgcagga gacgtcgctt tcacttgcat gcgcacgagc acatcgatgg
    11041 cctgccgata gcggttctcg agttgcggca ggccggcatg ggcacaggcc tctgacagcg
    11101 tgaggtctcc aaaatcctcg agatacagca ggccggctgt ctgatcataa tagtagaggt
    11161 cggggaccgg tacgccggcc ttggcaagat gcgcctgtat gttcacgaag ggtagctcgg
    11221 caatctggtg acccgcgccg ctcacggcct cttccgactg cttgaaggcc tcgggctcgg
    11281 ccaactgcat caggatgacc gactgagcgg catctccgag ctcaagtcga aaatagcgcc
    11341 gattcgaggc atccccggcg agaggggaca gtttcttgaa cgtcgctcga acaggcaacc
    11401 gggtccgtat ggtttccgcg atgagcgctc ggtccggagg agcgagggac tttttggtcg
    11461 tggcgggatc gatcgtggcc atcagcgcgg cattatactg ttgcgtgaaa aacttgtcac
    11521 gaaatgctcg gcttggtgcc gcgttccatg aggcggcgtg accccactgc gccgatcacc
    11581 gctccggtcg tatcggcgat ccaatcgacc acactggact cgcggagagg cacgtaggct
    11641 tgatggagct catcggtcag gccatacaag gaactcgcga cgatcgcaag cccgacggcg
    11701 tactctccgg cgtgcggtcc cgcggcccaa cgaaatgccc gatagcagag cagcgccagc
    11761 accgcatact cgatcccatg caacagcttg tcgcccagtt gttcaaagag gaagttgggc
    11821 agcctctcct cggggtgtga cagcgatgaa aggtaaaaga ttagggtgac gtacgcgatt
    11881 accgggaacc agtagcgcag ccattgcgcg gccgggctct gaatactttc cgcataggtc
    11941 ttttgcattt tcctccgttg attgccacct tcttatccct atgacatact ctgtgctact
    12001 ccacaatatc ctttacacac attgcctatg aaaaccgtcc gagtctgttt tctctggcat
    12061 atgcatcagc cctactacac cgacccggtg agtgggtcgg tgagcatgcc ctgggtccgc
    12121 ctccatgccg caaaggcgta ctttgacatg gcgtatctga ttgatcagtt tccacgggtg
    12181 cacgccacgt ttaattttac gccgtcatta ctggttcaat tacaagagat cggggacggc
    12241 tccgttcagg atctgttctg ggaacgtacg cagcggcagg ccgcccgctt gaacgaagaa
    12301 gaacaggcat tcctcatcaa gcacttcttc tcaatcaatt ggacgaccat gatccgaccg
    12361 taccctcgct atcacgaact attgaccaag cgtgggtttg acattgacgg gcaggaactc
    12421 catcggcttg cccggcagtt cacgacgcag gacttcctgg atttacaagt ctggttcaac
    12481 ttggcctggt tcggctatgg cgccgtcgcc cgctatccac gactcaccgc attacgatcc
    12541 aaaaatcggg gcttcaccga agaagaaaaa caggaggtgt tggcgttaca gcgcaggacg
    12601 gtccaggata ttgtgccgat gtaccggcgg ctggccgagc gcgggcagat cgagttgacg
    12661 acgacgcctt tctatcatcc gattctccca ctggtcattg acaccgagca cacgcgccgg
    12721 gccagaccgg atcttccact cccttcacga ttgagagcgc cagatgatgc cgaggcacag
    12781 ctgcgcctgg ctgtcgaata ccatctgagc gtgtttggca ggcctccggc aggactttgg
    12841 ccctccgaag ggtctgtctg ccccgaattg attccgctcc ttcgccgtgt cggcctccgt
    12901 tggctcgcca ccgacgaagg catcctgacc cggtcactcg aagcttcgga agtgccctgg
    12961 catcgggaga cccagctcta tcggccctat cgcgtaggac cgactggaga agaggcaacg
    13021 atcattttcc gggaccgtga cctgtccgac gggtttggct ttctctacca taagacgaca
    13081 ccccacgcgg ctgcagatga tgtcctacga cgcatgaagc ggatcgcagt cgacacgcca
    13141 caggacaacg tcctgcttcc catcatctta gatggcgaaa atccatggga gcattatcag
    13201 gaaggcggcg agcagttcct ctcgcatctc tatcaggccc tttcggatgg tgcgctcaac
    13261 gaaggcgact cgatttgtgt aaccaccgcc accgtttcca acgcgctgga ggccacgccg
    13321 gatcggccct atctcgcgca cctgcattcg ggctcatgga tcaaccagga ttttaagatc
    13381 tggatcggcc acgccgaaga caaccgtgga tgggatctgc tgaatcacac acgctcgcgc
    13441 ctgctcgaaa tcagttcgac gttgccgtcg aatcgtgcga gagcggcctg gcaagagctc
    13501 tacgccgccg aaggcagcga ctggttttgg tggtacggcg acgatttcga tacgacctat
    13561 aaggaagagt tcgattggct ttttcgcatg catcttcgca acgtctggac gctggccggc
    13621 atgacgccac cggatctgct caaccagccg ctgtgcgaga tccggcggtt cgagggccgc
    13681 gaccaggtca cgatgcctct ggccatctta catcccagac tggatggatt ggtgacggat
    13741 ttcttcgaat ggcgaggagc tgggacgatt aacccgaatc caccgctggg cgcgatgtgg
    13801 aaagcggaag gcctgtttac cgccattttt ttcgtctgcg atttcgagca cctctatctg
    13861 cggctggatc cggacgaggc gttcaagagc cgaaagagcg atctccgtct tgaatgtgtg
    13921 ctgcaggctc ctcgcggtgc gtatcgcctc ataatctcct ctgcctcccc ggatcgctat
    13981 cgattggccc aacgacgtga tgacggcaac tggggagaca tcggttcctg cgaactgatc
    14041 ggctggaacc aggtgttgga actggcagtt ccgttcaaag agattcaggt tggggagagc
    14101 gaacgcgtgc agttgagtct cctcgtgtac gagcatgaac tggaaatcgc ccgctacccg
    14161 cgacatcagc cggccatcgt gacggtaccc ggaccggaat tcggagcgac agtctggaga
    14221 gtatgaacag ccctctttac gcgttggtgc gtcatgcctg acttacggcg cgatccgatt
    14281 gtgggtcgct gggtcatcat ttcgacggac cgaaacggcc gtccgcatga ctttgcgcag
    14341 atgcagcccg tccggccgat ctcgactgcc ctgtgcccgt tttgtccagg gcaggaacgg
    14401 ctgactccga aagagatcat ggcctaccgg cctgaaccag ccgaggcaaa cgccccgaat
    14461 tggtccgtgc gggtggtgcc caacaagttt cctgccttgc aggtcgaagg agatttaggc
    14521 cgtgaaggca tgggacttta cgatcgcatg aacggcattg gcgcccatga agtcatcatt
    14581 gaggctccgg ggcataaaga cagtcttgcc gacatgccga ccaaccggct cgaagatgtg
    14641 ctctgggcct atcgcgaccg gattatcgat ctcaaaaggg atgtccgcct gcggtacatt
    14701 ctgatcttca agaaccacgg cgcggcggcc ggcgcgacac tcgaacacag ccactcccag
    14761 ttgatcgcgc tgccggtcat cccgaccagc gtgatgaacg agatcgaagg ctgccgtcta
    14821 cactatcaac aaaaagaacg gtgcatgtat tgcgacatct tgaagcagga ccttgcgcag
    14881 ggagaacgca ttgtggcgga gaatgccgag tatgcctgcg tgacgccgtt cgcaccgagg
    14941 tttccctttg aaatgtggat tcttcccaag cggcatgccg gtcactttga acaaagcgag
    15001 aaaaagcact ttgagtttct tgcaccgatt ctatcggaat cactccgtcg gatggatcgg
    15061 gtcctctcaa agccggccta caattttgtg ctccatagct ctccggtgca cgaaacaacg
    15121 ggggaatttt atcattggca catcgaactc attccgaaac tcactcaggt tgccggtttt
    15181 gaatggggct caggctttta catcaatccg gtctcgccgg aagagtccgc aagatatctg
    15241 cgcgaagcgg tcctctagac tttctctcct tgctcccgtt catggagcag gcttaagata
    15301 ccggcatgca cgtccaattg agtcatccgg caaggaccgt cgaaatcaaa gggccgaagc
    15361 ggacgaagga ccttttgcgg gaactcaacc tcgtggtaga agcgcatctt gtcatccgtg
    15421 gcgacgaact ggtgaccgaa gacgagatgc tctctgacaa ggaccaaatc gaaattaggc
    15481 cggtcatctc aggcgggaca ccttagcagc gcgacccacc acacattcac tgtggccgtt
    15541 tccactcccg cggggttact ggggcgggaa atgagagaga atgtttcgaa cgatcgcttt
    15601 cgtacgctcg tcccgttgct cggatgtcag gccctgatcg acctctccgg cagcagttcc
    15661 gcgccacacc aacttctgcg atgcgccatc gatgacgtcg atgagcagtg tgcctgtgag
    15721 ataggagtgc ccctcgctat tcgagggtgt cgtatcacgg acagaacggc tatcggcgga
    15781 atgcagaaag ggacgcccgg ccatcccctc ggaaaaatat tgtgacgaaa ccgtcggact
    15841 gctgtccttc aatgcgatcg tgtacgtcac taagaagtcc ggcctgccct ctggaagcgg
    15901 ttggtagccc ttcaagcgca attgggtccc gatggcgatt cgaatgcgca tatccaccgc
    15961 cgagttgtcg gcgcgtcgat cgccggtttt ttcctgctcc cccgataccc acgcatacgt
    16021 tttgtatccg ccgaagttgg tgctgcgatc gaagtcatac cccgtttttg gcaaggtggt
    16081 gcacccggca accacgagta gaacaccgct aatcaagagt ctccgcgtca tcaccgtatt
    16141 ctcatgcaag tgccgggcac atttattgag gtggaaaatg cgcaagcatc gcacggacga
    16201 tagtgctgat gcgttcatca cgttctttgg
//
LOCUS       DASTQY010000164         5373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_240266, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000164 DASTQY010000000
VERSION     DASTQY010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240266"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VFR82_05335"
     CDS             <1..1005
                     /locus_tag="VFR82_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU4501055.1"
                     /translation="GDGIAMALRAGAVLQDMEFVQFHPTALYLPSSPPFLLSEAMRGE
                     GAQLRNNKGEIFMPRYHPLGALAPRDIVSRAIWAEMAATKARHVYLDVTHLGAEFVKR
                     RFPTIYATCLRYDIDITEEWIPVSPSAHYMMGGVWTDLNGATTVPGLFAAGEVACSGV
                     HGANRLASNSLLEGLVFGARAATAAVAYAGREEIPSLATHEAALKHEQYGSLDDVEKL
                     RSSLRRTMWGQVGVIRSGESLIRACAQLSRWAQLVAQPFSQRTALEVKNMVQVAQCIA
                     EAALWRENSVGAHYRSDFPQAKRPGWQQHSRLSLGEGISGTPPRKSRGLLLALKGRAG
                     "
     gene            complement(941..1648)
                     /locus_tag="VFR82_05340"
     CDS             complement(941..1648)
                     /locus_tag="VFR82_05340"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU4501056.1"
                     /translation="MPMTAKLRRIATLIRRWSPGMLLTGATFLSPLHPVGAHAEIYQY
                     IGPNGTISLTNVPSDARYRRLDVESARFHALLSERELEPVIKRHSSQQQLHPALIRAV
                     IKAESNFDPRAVSRAGAIGLMQLMPQTAVRLDVRDMFDPDENVGGGAKYLRQLLDRFH
                     GNLPLALAAYNAGENIVDRYQALPPIDETRQYVRKVLRYYRTFLVRDGVITERPVSRH
                     APSAPAEAPATSVEASR"
     gene            complement(1755..2630)
                     /locus_tag="VFR82_05345"
     CDS             complement(1755..2630)
                     /locus_tag="VFR82_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEU4501057.1"
                     /translation="MGWIYLTDRFVKEEEALVSVFDHGFLYGDGVYETIRSYGHKIFM
                     RDQHLARLRRSADGIGLSIPQLDWPSVLHEAMARNGVGNEQTDAYIRVTISRGVGDIG
                     LDPALCPTPTIVIMAKPLKPPPQERYRTGVSLIVAKTRRNLPSALDPQIKATNFLNNI
                     QAKREALNTGAFDSVLLNWESHLTECTVSNLFFVRSGRLYTPALSCGILDGITRGVLL
                     GLARESGIPVEEGQFTVESLQTAEECFLSNTTMEVMPATQLNGRPIGNGMPGPLSKRL
                     HQIFVTHRDKFLESE"
     gene            complement(2630..4201)
                     /gene="pabB"
                     /locus_tag="VFR82_05350"
     CDS             complement(2630..4201)
                     /gene="pabB"
                     /locus_tag="VFR82_05350"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase, component I"
                     /protein_id="HEU4501058.1"
                     /translation="MAGAQFKQSTRELVKLRCGKLPNPWGIVNGSCDNPTSTMTRFSQ
                     PSFLQGAPRPLVLSFSGRGESPFDLYQKVASSTQPSFLLESGKGEVNRHYSFLGSRPA
                     SVLPRPALAPGLSSDDAFLWLRQTIALPGIERTPDLPPFFGGAVGCLSYDFARRLESL
                     PAVALDDLSLPDMHFALYEVVAAIDHRTNQIQVIFCPSLDRFLGEARDKLYREGLDRL
                     AEWEARLTGSVATPPDPLALTTVSFGPQQTREAYLDRVRRCQDYIAAGDIYQANLSHR
                     FAIEIPQDYRSGAGRLQYEQALYQQLRSVNPSPFSGLLRFGDMSLISCSPERLVRLQG
                     RRADTRPIAGTRPRGRNPDDDRRLVRELLANDKERAEHVMLVDLERNDLGRVCEIGSV
                     HVNEFMAVEQYSHVSHIVSNVQGTLRPEATPFDLIGATFPGGTITGVPKIRCMEIIEE
                     LEPVRRGPYTGSFGYIGWNGDLDLNIIIRTLVMTGRQGYLQVGAGIVADSDPAGEYQE
                     TLHKAQAFFSAVGHR"
     gene            4249..4467
                     /locus_tag="VFR82_05355"
     CDS             4249..4467
                     /locus_tag="VFR82_05355"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEU4501059.1"
                     /translation="MPQSQDPFYAGLGQAVRIGTELLAALIVGGGLGWAVDTYLLETG
                     PWGLVLGLILGAAAGVRNAYRMAQRWPQ"
     gene            4494..5243
                     /locus_tag="VFR82_05360"
     CDS             4494..5243
                     /locus_tag="VFR82_05360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU4501060.1"
                     /translation="MEDSPLHPFELHNYIPINLGGLDISINKAVIMMWVVVGLISILM
                     VMAGSARRLVPGKLQSLAEMLVDFIRGIILDTMGKDGMKFFPLIATLFLFILFCNLIG
                     LIPGSYTVTSQIVVTAVFACTVYGLSLLMGFVLHGGKFFGILVPPGTPGWLLPLMVPI
                     ELISQLARPISLAVRLFANMTAGHVILGVLFGLAVSGGLLIGWLPFAFTIAMNGLEVG
                     IAFIQAYIFTVLTCVYLGDAFHLHGHDEHAH"
BASE COUNT         1081 a         1470 c         1669 g         1153 t
ORIGIN      
        1 ggagacggca tcgccatggc gctgcgagcc ggcgccgtcc tgcaggatat ggagttcgtg
       61 cagtttcatc ccacggcgtt gtacctaccc tcgagccccc ccttcctatt gtccgaggct
      121 atgcgcggag agggggctca actccgaaac aacaaaggcg aaatcttcat gccgcggtat
      181 catccgctcg gagctcttgc tccgcgtgac attgtgtcgc gtgcaatctg ggccgagatg
      241 gccgccacaa aagcccggca cgtttacttg gacgtcacgc atcttggcgc cgaattcgtc
      301 aagcgtcggt ttccgacgat ctatgccaca tgcctgcgtt atgacatcga cattaccgag
      361 gagtggattc ccgtgtctcc cagcgcccat tacatgatgg gcggcgtatg gacggacttg
      421 aatggcgcca cgaccgtccc gggattgttt gcagcgggcg aagtggcgtg cagcggcgtc
      481 catggggcga accgactggc aagtaattca ttactcgaag gactggtctt tggcgcgcgg
      541 gcggctactg cggcggtagc ctatgcgggc cgtgaagaaa ttccgtctct ggctacgcac
      601 gaagcggctc tcaagcacga acagtatggt tcgcttgacg atgtggaaaa attgcgcagc
      661 tcacttcggc gcacgatgtg gggtcaggtg ggggtgatcc gatccggcga atcgcttatc
      721 cgggcatgcg cgcagctctc ccgctgggca caactggttg cacagccgtt ttctcagcgg
      781 acggcgctgg aggtcaagaa catggtgcag gttgcgcagt gtattgcgga agccgctctg
      841 tggagggaaa acagcgttgg ggcccattac cggtcggatt ttcctcaagc caagcggccg
      901 ggatggcaac agcacagtcg gttgtcgctg ggcgagggaa tcagcgggac gcctccacgg
      961 aagtcgcggg ggcttctgct ggcgctgaag gggcgtgccg gctgacggga cgctcggtga
     1021 tgacaccgtc gcgaacgagg aaggtccggt aatagcgcag aaccttgcgg acgtattgtc
     1081 tcgtttcgtc gatcggcggc aacgcttgat aacgatcaac gatgttctcg ccggcattgt
     1141 aggccgccag ggcgagaggc aaattcccgt ggaagcggtc gagtaactgc ctcaagtact
     1201 tcgcgccccc cccgacgttt tcatcaggat cgaacatatc ccggacatcg aggcgtacgg
     1261 cggtttgcgg catgagctgc ataaggccga tcgctccggc gcgggaaacg gcacgtgggt
     1321 cgaaattcga ctcggccttg atgactgccc ggatcagcgc cggatgcaat tgctgttgag
     1381 acgaatggcg tttgatgacg ggctctaatt cgcgttcgga aagcaaagcg tgaaagcggg
     1441 ccgattccac gtcgagcctc cgataccggg cgtctgaggg cacgttggtg agcgagatcg
     1501 tcccgttcgg tcctatatat tggtagattt ccgcgtgtgc gccaacaggg tggagcggtg
     1561 acaggaacgt ggcccccgtc aggagcattc cgggactcca tcggcggatt aaggtggcga
     1621 tgcgtcgcaa tttggctgtc atcggcatgg tccccacgat agcagtctgt tccagcctgt
     1681 caagaaaatg cagatcccat gccgcatggc agcgcaggaa acaggaggca gaacaagagg
     1741 atcggacggg aagcctactc agattccaga aatttgtcac ggtgagtgac aaaaatttgg
     1801 tgcagccgtt tgctgagtgg gccgggcatt ccgttcccga tcggccggcc attcagctgc
     1861 gtggccggca tgacttccat tgtcgtgttg ctgagaaaac actcctcggc cgtctgaagc
     1921 gactccacgg taaattggcc ttcttcgaca ggaatccctg attcgcgcgc caaccccaga
     1981 aggactcctc gtgtaatgcc atccaaaatg ccacaggaaa gagccggggt gtagagccgg
     2041 ccggatcgca cgaagaacag attgctgacc gtacactcgg tgaggtgaga ttcccaattg
     2101 agcaacacgc tgtcaaaggc cccagtgttg agcgcttctc gtttcgcctg aatgttgttc
     2161 agaaaattcg tcgccttgat ttgtgggtcg agagcgcttg gaagatttcg cctggtcttg
     2221 gccacgatca gactcacgcc ggtccgatac cgttcctgcg gaggcggttt gagcggtttg
     2281 gccatgatca cgatcgtcgg agtggggcag agcgccggat cgagaccgat atctccgacc
     2341 ccccgggaga tcgtcacgcg aatgtaggcg tcagtctgtt cattgcccac gccgttgcgg
     2401 gccatcgcct cgtggagaac cgatggccag tccagctgtg gaatggacag gccaatgccg
     2461 tctgccgagc gtcgcagacg agccaggtgt tggtcgcgca tgaagatttt gtggccgtac
     2521 gagcgaatcg tttcgtacac gccgtcaccg tagagaaagc catggtcgaa tacggagacg
     2581 agcgcctcct cctccttgac gaaacggtca gttaagtaaa tccaccccat cagcggtgtc
     2641 cgaccgcgct gaaaaatgcc tgcgccttgt gaagagtctc ttgatattcc ccggcgggat
     2701 ccgagtccgc gacgatgccg gcccccactt ggagatatcc ctgccgcccg gtcatcacaa
     2761 gcgtgcggat aatgatgttc aaatccaaat ccccgttcca cccgatatag ccgaatgatc
     2821 ctgtataggg tccgcggcga acgggctcga gttcctcaat gatctccatg caacggattt
     2881 tcgggacgcc cgtgatggtc ccgcccggaa acgtggcgcc gatgagatcg aagggggtcg
     2941 cttcgggacg cagcgtgccc tgtacgtttg acacaatatg gctgacatgt gaatactgct
     3001 ccaccgccat aaattcattg acgtgcacgc tgccgatttc gcaaacacgg ccgagatcat
     3061 tccgttcgag gtcgaccaac atcacgtgtt cggccctctc cttgtcgttg gcgagcaact
     3121 cccggacgag acggcggtcg tcatcgggat tgcggccacg gggtcgggtg ccggcgatcg
     3181 gtctggtgtc agcacggcga ccttggaggc ggaccaagcg ctccggcgag caactgatca
     3241 gcgacatgtc gccgaaccgc aggaggccgg aaaaagggga tggattcacc gagcgaagtt
     3301 gttgatagag cgcctgctcg tactgcaaac ggcctgcccc cgatcggtag tcttgtggga
     3361 tctcgatggc gaaccgatgc gagaggttgg cctggtagat atctccggca gcaatgtagt
     3421 cttggcagcg gcgaactcga tcgagatagg cctctcgcgt ttgttgcgga ccaaatgaga
     3481 cggtggtcag cgccaacgga tcaggcggtg tcgccactga gccggtaagt ctggcttccc
     3541 attccgcaag ccggtcgagt ccctcacgat aaagtttgtc gcgagcttca ccgagaaatc
     3601 gatcgagcga tggacaaaag ataacttgaa tttgattcgt tcgatgatcg atcgcggcga
     3661 ccacctcgta gagtgcgaag tgcatgtcgg gaagtgagag atcgtcaagg gcgactgccg
     3721 gcaaggactc caaccggcgc gcgaaatcat aactgaggca tccgactgcc cccccgaaaa
     3781 acggcggcag atcgggcgtt cgttcgatgc cgggcaaagc gatcgtttgc cgaagccata
     3841 aaaaagcatc gtccgatgat aatccggggg cgagggcggg tcgcggcaag acagaagccg
     3901 ggcggctgcc caaaaatgag tagtgcctgt ttacctcgcc cttcccgctt tccaagagaa
     3961 aggagggttg tgtggacgag gcgacctttt gatacaggtc gaatggactc tcaccccgac
     4021 cgctgaatga taggacaagc ggccgtgggg caccttgtag gaaggaaggc tgggaaaatc
     4081 gggtcatcgt cgaagtcggg ttatcgcagg agccgttcac tataccccac ggattcggta
     4141 atttcccaca ccgcagcttg acaagctcac gagtactctg cttgaattgc gcgcccgcca
     4201 ttcggcagag tcctgattca ggacactcgc ctcagtgggt taccgaccat gccccagtca
     4261 caggatccgt tttatgcggg gctcggccag gccgttcgga tcggaacgga actgcttgct
     4321 gcgctgattg tggggggcgg actgggctgg gccgtcgata cctatctgtt ggagaccggt
     4381 ccctggggcc tcgttctcgg cttgatcttg ggtgcggcgg ccggcgtgag gaatgcttat
     4441 cgaatggcgc agcggtggcc tcagtaaccg attagcgcat cgcgaaaggg ttcatggaag
     4501 atagtcctct ccatccgttt gagcttcaca actatattcc gatcaatctc ggcggcctgg
     4561 atatttccat caataaagcc gtcatcatga tgtgggtggt ggtgggactt atttccatcc
     4621 tcatggtcat ggcggggtct gcccgccgac ttgtgcccgg caagctgcaa agcctcgccg
     4681 agatgttggt tgattttatt cgcggcatta ttctggacac gatgggaaag gacggcatga
     4741 agttcttccc cctgattgca acgctgtttc ttttcatttt gttttgtaat ctcattggat
     4801 tgattcccgg ttcttatacg gtgaccagcc agattgtcgt gacggcagtg tttgcctgca
     4861 ccgtctacgg actcagcctc ctgatgggct tcgtgctgca tggtgggaaa ttctttggga
     4921 tcctggtgcc acccggtacc ccgggctggc tgctgccttt gatggtcccg atcgaactca
     4981 tcagccagtt ggctcgaccg atctcgcttg ccgttcggtt gtttgccaat atgacggcag
     5041 ggcacgtgat cctgggcgtg ttgttcgggc tggccgtcag tggaggctta ttaatcgggt
     5101 ggttgccgtt cgcgttcacg atcgccatga acggcctgga agtcggcatc gcattcatcc
     5161 aagcctatat atttacggtg ttgacctgcg tgtatttggg cgatgcgttt catttgcatg
     5221 gccacgacga gcatgctcac taacacggag tgacgcgtag agggcgtgga gcgaggcagt
     5281 ctcagtttgc aacggaccta tttagccagg gaggagtacg agacgatgga ttcagcagcc
     5341 gcagcacttt tgggcatggg acttgccgcc gcc
//
LOCUS       DASTQY010000165         5408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1547123, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000165 DASTQY010000000
VERSION     DASTQY010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547123"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFR82_05365"
     CDS             <1..500
                     /locus_tag="VFR82_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEU4501061.1"
                     /translation="AVATRLFKRLQLISACFMAFSHGANDAQKAMGIITLALLSAGQI
                     PSAEVPTWVIGACAVAMGLGTAAGGWRIVRTLGMKIVKLEPVHGFAAETAAATVLMFT
                     AHIGLPVSTTHTITSSVMGVGAIKRLSAVRWGVTRRILYAWLFTLPGAAIISTAIYLI
                     MGLFL"
     gene            complement(518..2323)
                     /locus_tag="VFR82_05370"
     CDS             complement(518..2323)
                     /locus_tag="VFR82_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4501062.1"
                     /translation="MSLSIRWKVTLAILFALLCGFVIAGVLAARSLAEQELAQSGEAL
                     QTSTHLIALDLRPLVTASSLPPIPQLQTIVRELSQRARARVTLIDASGRVLADSAVRD
                     GDLAGIENHRTRPEIEQAVATGSGTDLRASHTTGERTLYRAVRLGDRGAASAPLYLRL
                     GLPMTALEHELTTLQRNLTLAFGSAFLIAVGLSVWLARSLTKPLSDMAAAARQLAGGN
                     LSVAVQTSSRDEVGLLADTLNHMTRELRTKIEELSEDRSQLLAMLTSMVEGVMVLDSK
                     GRILQVNPAWERMFGVTRNEARGRQSSEVFGHPKLNTLVSAVLAARTGQEGEILLPSG
                     RCLNVEASVAGGEQDNEACAVLVFHDITELRRLEHIRKDFVANVSHELRTPLTSIKGY
                     VEALLDGGKDDPETSVRFLDIILKQSDRLNLILEDLLQLSKIESGQVQFKQEPLHIGS
                     VIERTIAMIKPLADKKQHRLIAQVAADLPLITGDQERLVQVLANLLDNAIKYTPEGGQ
                     ITLSARRLPATRTDGPGDGVELMVSDTGIGIPEADRPRVFERFYRVDKARSRELGGTG
                     LGLAIVKHIVEGHGGQIHVEANVPSGSRFVVRLPA"
     gene            complement(2352..3053)
                     /locus_tag="VFR82_05375"
     CDS             complement(2352..3053)
                     /locus_tag="VFR82_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4501063.1"
                     /translation="MASVPAKKILIIEDERDILQLVKLYLEKEGFRTVTATTGAEGLN
                     SAKQNKPDLIVLDLMLPEIDGLEVCKRLRSAPETAMLPIIMLTAKAEESDQVIGLELG
                     ADDYVTKPFSPKALVARVKALFRRTDRSRQESQTAYQYGKLAMDLDRHEVTVQGKDVP
                     LTAKEFGLLEHLLRHPGRVLTREVLLNQVWGYDYFGTTRTVDVHVRRLKQKIPLLDEA
                     ILSVKSLGYKLKVLD"
     gene            3281..4240
                     /gene="ispH"
                     /locus_tag="VFR82_05380"
                     /gene_synonym="lytB"
     CDS             3281..4240
                     /gene="ispH"
                     /locus_tag="VFR82_05380"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEU4501064.1"
                     /translation="MKIYLANPRGFCAGVDRAIDIVDLSLKKYGAPIYVRHEIVHSRH
                     VVNSLRHKGAVFVEELNEVPEGSVVIFSAHGVAKSVWDEANRRRLHVIDATCPLVIKV
                     HNEVNRDYTQGYDLILIGHAGHPEVIGTLGQVPDKFHLVSSVEDVERLLVQNTTNLSY
                     VTQTTLSVDECRDIVGALHKRFPHIKGPHQEDICYATQNRQNAVKELSKLVDVILVIG
                     SPNSSNSNRLRELGEHCGISSYLIDSAADINSEWVKNAKAIGITAGASAPEVLVEEVV
                     AYLKTYGSAEVEDLTVIEEDVEFLLPKELITIAPASKSVAASR"
     gene            4407..>5408
                     /locus_tag="VFR82_05385"
     CDS             4407..>5408
                     /locus_tag="VFR82_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4501065.1"
                     /translation="MYLKSLEMVGFKSFAEARLEFPNGVTAIVGPNGSGKSNVVDAIL
                     WVLGEQSTKTLRSEKMEDVIFNGTELRKPLGMAEVSLVISGLDQVKVEGQNGLPGQLS
                     EYQDLMITRRLYRNGDSEYLINKTQCRLKDIRSILLDTRAGTKGHTVIAQGQIDQILN
                     ASPQDRRELIEETAGIVRYKKQKAEALRKLDATQQNLLRVRDIIAEVKKQLNSLERQA
                     RQARSYQVLQEEARTLEVQLLTNELRRLRQTIADIERELATLSGQESEQAAHQARLSA
                     ELERLKLSIADTGLVIGQRREELSHIEQRQAQALTAAEVERHKGELYEQQRQQGAEDR
                     "
BASE COUNT         1140 a         1568 c         1588 g         1112 t
ORIGIN      
        1 gggcagtggc cactcgcctg tttaagcgat tgcagctgat ctccgcttgt ttcatggcct
       61 tcagccatgg ggcgaatgac gcgcagaaag ccatggggat cattacactg gcattactct
      121 ccgccggtca aattccatca gcggaagtgc cgacctgggt gatcggggct tgtgcggtgg
      181 caatgggatt gggaaccgcc gccggcgggt ggcgcatcgt gcgcacctta gggatgaaga
      241 tcgtaaagct tgagcctgtg cacggctttg cggcggagac cgccgcggca accgtcttaa
      301 tgttcaccgc ccatatcggc ctgcccgtga gcacgaccca tacgatcacg tcatccgtca
      361 tgggggtcgg cgcgatcaaa cggctgtcag cggtgcggtg gggagtgacg agacgaatcc
      421 tgtatgcctg gctgttcaca ctccctggcg ccgccatcat ctcgaccgcg atctatctga
      481 tcatggggct attcctgtag gagaccgatg caaggcgtta agccggtaag cggacaacga
      541 agcggctgcc ggatggaacg tttgcttcca cgtgaatctg tcctccgtga ccctcgacga
      601 tgtgcttgac gatggccagt cccagcccgg ttcctcccag ttcccgtgac cgcgccttgt
      661 ccacgcggta gaaccgttcg aacacgcgcg gccgatccgc ttcgggaatg ccgatgccgg
      721 tatcactcac catgagctcg acgccgtctc cgggcccgtc ggtccgcgtc gcgggaaggc
      781 gtcgcgcgga aagcgtgatt tggccgccct ccggcgtgta tttgatcgca ttgtcgagca
      841 gattggccag cacctgaacc aaccgttctt ggtcgccggt gatgaggggc aaatcggcgg
      901 cgacttgcgc gatgagccgg tgttgcttct tgtcggccaa cggcttgatc atcgcgatcg
      961 tacgctcgat cacagatccg atgtgcagcg gttcctgttt gaattgcacc tgccccgact
     1021 cgatcttgga cagctgaagc aagtcttcga gaatgagatt gagccggtcg ctttgcttta
     1081 aaatgatgtc gagaaaacgc acgctcgttt cggggtcatc cttccctccg tccaagagcg
     1141 cctcaacata gcccttaatc gacgtgaggg gcgtgcgcag ctcatgcgac acattggcga
     1201 cgaagtcttt cctgatatgt tccagccggc gcagctcggt gatgtcgtgg aaaacgagga
     1261 cggcgcaggc ttcattgtcc tgttctccgc ctgcgaccga cgcttccaca ttgaggcagc
     1321 gcccacttgg gagaaggatt tcgccttcct ggccggtgcg cgccgcgagc accgcggaaa
     1381 cgagcgtgtt caatttggga tggccgaaca cctcagaaga ttggcgtccg cgcgcctcat
     1441 tgcgcgtcac cccgaacatg cgctcccacg ccggattcac ttggaggata cggcccttgg
     1501 agtccagtac catgacccct tctaccatgg aggtgagcat ggccagcagc tgagaacggt
     1561 cttcggacag ttcctcgatt ttggtccgca attcgcgggt catgtggttg agggtgtctg
     1621 ctaaaagccc cacttcgtcc cgcgaggatg tctgcacggc gaccgacaga ttgccccctg
     1681 cgagttgccg tgcagcggcc gccatatcgg acagcggctt tgtcaggctt cttgccagcc
     1741 agacgctcaa tccaaccgcg atcaaaaagg cgctgccgaa agccaacgtc aaattccgtt
     1801 gaagggtcgt cagttcatgt tccaaggcgg tcatcggcag gcccagcctc aaatagagcg
     1861 gagcagatgc tgcaccgcga tcaccgaggc gaacggcccg atacagtgtg cgctcgccgg
     1921 tggtgtggct tgcccgaaga tcggtgcccg aaccggtcgc gacagcttgt tcgatttcgg
     1981 gccttgtgcg gtgattctca atgcccgcca aatcgccgtc ccggacggca ctgtccgcga
     2041 gcacccgtcc ggaggcgtcg atgagcgtga cgcgcgcacg cgcgcgctgg ctcagctccc
     2101 gaacgatcgt ttgaagctga ggaattggcg gaagggagga tgccgtgacg agcggacgca
     2161 ggtcgagcgc aatgaggtgg gtagaggttt gcagggcctc gccggattga gccagttcct
     2221 gttctgccag cgatcgggct gctagtactc cagcgatgac aaacccgcag agtaaggcga
     2281 acagaatggc aagggtgacc ttccagcgga tggaaagact catacaagcc gtgcaaggct
     2341 gtcaacgatt ctcagtccaa gaccttgagt ttgtaaccta gtgatttcac cgacaggatc
     2401 gcctcgtcga gtaaagggat cttctgcttc agccggcgaa cgtgaacgtc gaccgttctc
     2461 gttgtgccga aataatcgta accccagacc tgattgagca gcacttccct cgtcaggacc
     2521 cggccgggat gacgcagcag atgttccaga agtccgaatt ccttggccgt aagcgggaca
     2581 tcctttccct gtaccgtgac ctcgtggcgg tcaaggtcca tggctagctt gccgtattgg
     2641 taggcggtct ggctttcctg ccggctgcga tccgtgcggc gaaacagcgc tttgacacgc
     2701 gccacgaggg ctttagggct gaacggcttc gtgacataat cgtcggcgcc gagttccaga
     2761 ccgatgacct gatccgattc ctcggccttt gccgtcaaca tgatgatcgg cagcatcgcc
     2821 gtctccggag cggaacgtaa acgtttacag acctcgagac catcgatttc cggcagcatc
     2881 aaatcgagga cgatcaaatc gggtttgttc tgcttcgcgc tattcaggcc ttcagccccg
     2941 gtggtggctg ttaccgtacg aaagccctct ttctccaaat acagcttcac cagctgcaga
     3001 atatcgcgct catcttcaat gatcagaatt ttcttggcgg ggacactggc catggaaggt
     3061 gcgaccagcg tacggggcag ggcagtcgct gtcaagctcc caggggtgac ctgccgtctc
     3121 gttcttttcg ggcgttctga gcgtcggctc cgtcttccct tggctctgac gccggcgcca
     3181 tcgtgctcct atctccagcg atttgggcgg ctattctgtt gacggaccct catgaactca
     3241 cgtaaggtga gtggatgtcc ggggcaggga agggacggtc atgaagatct atctcgccaa
     3301 tccacgcggc ttttgcgccg gggtcgaccg ggcgatcgac atcgtcgatc tttcgctgaa
     3361 aaagtacgga gcaccgattt acgtccgcca tgaaattgtc cacagtcgtc acgtcgtcaa
     3421 ttcgttgcgc cacaagggcg ccgtcttcgt cgaagagttg aacgaggttc cggaagggtc
     3481 cgtcgtgatt tttagcgcgc acggtgtcgc taaatcggtg tgggacgaag ccaatcgccg
     3541 ccggttacat gtcattgacg ccacctgtcc tctggtcatc aaagttcaca atgaagtgaa
     3601 ccgggattat acacagggat acgatctcat tctgatcggt catgccggcc atccggaggt
     3661 gatcgggaca ttaggacaag tgccggacaa gtttcatctc gtgtcgtcgg tcgaagatgt
     3721 cgagaggcta ttagtacaaa acacgaccaa cttgtcctat gtcacacaga cgacgcttag
     3781 tgtggatgag tgccgggata tcgtcggggc cttacacaag cggttccctc acatcaaagg
     3841 accgcatcag gaagatattt gctacgcgac gcagaatcgc cagaatgccg tgaaggagtt
     3901 gtcgaagctc gtcgatgtga ttctcgtcat cggttcaccc aacagctcga actcgaaccg
     3961 cttgcgcgag cttggtgaac attgcgggat tagttcgtac ttgatcgatt cagccgccga
     4021 catcaactcc gagtgggtaa agaatgccaa ggcgatcggc atcactgcgg gtgcctcggc
     4081 gccggaagtt ctcgtggaag aagtggtagc ctacctcaaa acctatggca gcgccgaggt
     4141 cgaggatttg accgtcatcg aagaggatgt cgagtttctc cttcccaagg aattgatcac
     4201 gatcgctccg gccagcaagt ctgtggccgc gtccagataa ccctcttcgc gacccttcat
     4261 ctcatcccaa gtatggtggg cattcagcct tccggccact acctgcggta tttttctttg
     4321 atttccttcg agccctgtgc tacaaaaccc acccgtggtt ttccctcgtc cggttagcct
     4381 ccgaccaact accaaaggag cagggcatgt acctcaagtc gcttgagatg gtggggttta
     4441 agtcgttcgc cgaagcacgt ctcgaatttc ccaacggcgt gacggccatc gtcggcccaa
     4501 atggcagcgg caagagcaac gtcgtggatg ccatcctctg ggtactcgga gaacagagca
     4561 caaagacgtt gcgcagtgaa aaaatggaag acgtcatctt caacggaacg gaattgcgca
     4621 agccgctagg catggccgaa gtatccctgg tcatcagcgg cctggatcaa gtcaaagtgg
     4681 aaggtcagaa cgggctgccg ggccagttgt cggaatacca ggacctcatg atcacccgcc
     4741 ggctctatcg gaacggggac agcgaatacc tgatcaacaa gacgcagtgc cgcttgaagg
     4801 acattcgcag tatcttgctg gatacccggg caggcacgaa ggggcacacc gtcattgccc
     4861 aagggcaaat cgaccagatt ctcaacgcct cgccgcagga ccggcgcgag ctgatcgaag
     4921 aaaccgctgg aatcgtccgt tacaagaaac agaaggccga ggcactacga aaattggacg
     4981 ccactcagca gaacttgctg cgcgttcggg acatcatcgc cgaagtcaaa aagcaattga
     5041 actcgcttga acgccaggcc cggcaagcac gctcctacca ggtgcttcag gaagaagccc
     5101 ggacgctcga ggtgcaattg ctgaccaatg aattgcgccg cttgcggcaa accatcgcag
     5161 atatcgagcg cgaactcgcc acgctcagcg gacaggaatc ggagcaagcg gctcaccagg
     5221 cccgtttgtc cgctgaactg gaacgcctca agctgtcgat cgccgacacc ggactcgtca
     5281 tcggccaacg acgggaagaa ctctcgcaca tcgaacagcg acaggcccag gcgttgacgg
     5341 cagcggaagt cgaacggcac aagggcgagc tctatgaaca gcaacgccaa cagggcgccg
     5401 aggatcgt
//
LOCUS       DASTQY010000166         6835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_127420, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000166 DASTQY010000000
VERSION     DASTQY010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127420"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VFR82_05390"
     CDS             <1..524
                     /locus_tag="VFR82_05390"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEU4501066.1"
                     /translation="KTNKEYQAHLFELQMANKKKSEIEDKVLACMEKIEQLQVAAKEA
                     EEKVRVAEQAFNKEKHVLDELDRKLSSELAELEGQQRERSAKVEPALLARYAKLKASR
                     KDQPLAAIQRGMCSGCRLQIPPQLIAEVKRSQDLHTCPYCHRMLYWDGEAQVDQKPLD
                     HTKPTDLEVGESV"
     gene            complement(536..1189)
                     /gene="rnhC"
                     /locus_tag="VFR82_05395"
     CDS             complement(536..1189)
                     /gene="rnhC"
                     /locus_tag="VFR82_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU4501067.1"
                     /translation="MPSSSIERIGIDESGKGDYFGPLVIAAVFVDATTQEELTLMAVR
                     DSKKISDGRILELAPDIKTICPHSVIAIGPQKYNELYGKIRNLNRLLAWGHAKALETL
                     LERVSCERAISDQFGDERLILNALQEKGRRIVLEQRTKAESDLAVAAASIVARAEFLL
                     RLKRLSDEIGTVLPKGASPAVELAGRMVVKKHGQERLGTVAKLHFKTTKAVLEEAAT"
     gene            1404..2276
                     /locus_tag="VFR82_05400"
     CDS             1404..2276
                     /locus_tag="VFR82_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU4501068.1"
                     /translation="MYDVRMKRHAVSIIGTTLSLSLAFASGCETNPYTGRSQLLMTSV
                     SQEMQLGAQAYNQVKNDPKMKPSQDSREIEPVKRVAARIVEAAKRSKYAEMAQQFQWE
                     VTVIKDDKTANAFALPGGKMAVYTGIFPMAKTEAGLAAVMGHEVVHALARHGAERMSQ
                     GQLTSAALKAAGAAAGASGMNPLLSQGTMAALGAGAQVGVLLPFSRKHESEADYIGIL
                     LAADAGYDPRESVALWERMAQMSGGGAPAEFMSTHPSHETRIDQLKKWLPEAMTIYQS
                     KQPVPAAELPPVGP"
     gene            2352..2775
                     /gene="rnpB"
                     /locus_tag="VFR82_05405"
     ncRNA           2352..2775
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFR82_05405"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2995..4770
                     /gene="ilvB"
                     /locus_tag="VFR82_05410"
     CDS             2995..4770
                     /gene="ilvB"
                     /locus_tag="VFR82_05410"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEU4501069.1"
                     /translation="MKLTGAEIFIECLKREGVKTLFALPGGVVLKIFDMLHQQKDIEV
                     VLTRHEQGAGHMAEGYAKATGKAGVCLVTSGPGMTNVITALADAYMDSVPLVCFSGQV
                     PTSLIGNDAFQEADNVGLSRPCTKYNFLVKDVNELASTIKEAFYIATTGRPGPVLVDI
                     PKDVSMDKAEFNYPNSVSIRGYNPTYEGNKWQVKQAAEAIMKSKKPILYVGGGVIFSG
                     ASKELIELAELTQIPVDMTLMGLGAFPGEHPLSLGMMGMHGTYQANMAVHYSDLVVAI
                     GARFDDRVTGKVSEFCPYAKIIHIDIDPTSIRKNIHVDIPIVGDCKAVLRELNQILRA
                     TVNGEQKELRKAWWDQIREWESAHPLSYQQEADGPIKPQQVIKRLYDLTKDLDPIVST
                     DVGQHQMWAAQYFKMAKPNRWLTSGGLGTMGFGFPAAMGAQAAFPGRLVLCIAGDGSV
                     QMNMQEMATAVVHKLPVKIIVLNNRFHGMVRQWQDLFYEGRYASSYLDTTPDFVKLAD
                     AYGAIGLRASKVSELDNVLKEAIAAKGPVIVDVPTHPYENVYPMIPAGGCNHEMILED
                     TPELKLKQSGGAKVTPQDKDTILTA"
     gene            4838..5356
                     /gene="ilvN"
                     /locus_tag="VFR82_05415"
     CDS             4838..5356
                     /gene="ilvN"
                     /locus_tag="VFR82_05415"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU4501070.1"
                     /translation="MEHIILVTVENKFGVLSRIAGLFSGRGFNIESLSVAPTLDPSMS
                     QMTIVTSGDERIVEQIVKQLNKLIDVIKVVDLNESDFVSRETALIKVHTRQEDRAEAL
                     RIADIFRANVIDSTPATYTIEVTGDPKKIEAIINLLQPLGIKELTRTGRVAVAREPLR
                     STTTQPKKVARE"
     gene            5411..6427
                     /gene="ilvC"
                     /locus_tag="VFR82_05420"
     CDS             5411..6427
                     /gene="ilvC"
                     /locus_tag="VFR82_05420"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU4501071.1"
                     /translation="MMKIYYDRDADLQQITNKKVAVIGFGSQGHAHALNMKESGVSVV
                     VGLREGSSWRKAEQSGLKVMPVADAVKASDVVMILAPDEAQAALYRQEVAPNLKAGSY
                     LAFGHGFNIHFGQIVAPSTINVFMVAPKGPGHLVRSEYTKGSGVPCLLAVHQDPSGTT
                     KQVGLAYASAIGGGRAGVIETNFREETETDLFGEQAVLCGGLTSLIQAGYETLVEAGY
                     SPEMAYFECLHEVKLIVDLIYQGGIANMRYSISTTAKYGDVTRGPRIVTEQTKQEMKK
                     ILGEIQQGQFAKEWVLENQANRPVYNALLAKGEAHPIEEVGAKLRAMMPWLKKDQLVD
                     KSKN"
     gene            6444..>6835
                     /locus_tag="VFR82_05425"
     CDS             6444..>6835
                     /locus_tag="VFR82_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEU4501072.1"
                     /translation="MADRAAGVPFAKEGIPFIGAAAGITLIAWWLDWVPVAVVGAVLT
                     LFVSWFFRNPPRRVPQGPGLVVAPGDGVVLAVEEEFEPRYLKDRAVRISIFLNVFDVH
                     INRIPCEGIVENVQYQPGLFLVASKPHAT"
BASE COUNT         1578 a         1801 c         2054 g         1402 t
ORIGIN      
        1 tgaagaccaa caaggaatat caggcccatc tgttcgagtt gcagatggcc aacaaaaaga
       61 agtccgaaat cgaggacaag gtgttggcct gtatggaaaa aatcgaacaa ctccaagtgg
      121 cggcaaaaga agcagaggaa aaggttcgag tcgccgagca ggccttcaat aaggaaaagc
      181 acgtactcga tgagttggac aggaagctgt cgtcggaact ggccgaactg gaaggccagc
      241 agcgggagcg atcggcgaag gtcgaaccgg cgttgcttgc ccgctatgcc aagttaaaag
      301 cctccagaaa ggatcagccg cttgcggcca ttcagcgcgg tatgtgttcc ggatgtcggc
      361 tgcagatccc tcctcaattg atcgcggaag ttaaacgctc tcaggatctg cacacctgcc
      421 cctattgcca ccgcatgttg tactgggatg gagaagcgca agtcgaccaa aagcccctgg
      481 atcatacaaa gccgaccgat ctcgaggtgg gagaatcggt gtaactctct ttggtctagg
      541 tcgcggcctc ctcgagaacg gctttggtcg tcttgaagtg cagtttcgcg acagttccca
      601 accgctcttg tccgtgcttc ttcacgacca tcctacctgc caattcaact gcaggcgatg
      661 cccctttggg taagacggtg ccgatctcat ctgagaggcg cttaagtcgc aacaagaact
      721 cggcccgagc cacgatcgag gcggcggcaa cggcgagatc cgattctgcc ttcgtgcgct
      781 gctcaagcac gatgcgccgc cctttttctt ggagcgcgtt gagaatcagc cgctcatcgc
      841 cgaattgatc cgagatcgca cgctcgcagg agacgcgttc cagcaatgtc tcaagtgcct
      901 tcgcgtgtcc ccaagccagc aaacgattga ggtttcggat cttcccatag agctcgttgt
      961 atttctgggg accaatggcg atgacgctgt gaggacagat cgtcttgatg tcaggggcga
     1021 gctcgagaat gcgtccgtcc gagattttct tgctgtctcg caccgccatg agcgtgagct
     1081 cctcttgagt cgtcgcgtcg acgaacacgg cggcaatgac gagcgggccg aagtaatcgc
     1141 cctttcccga ttcgtcgatt cctatgcgtt cgatcgaaga ggacggcatt ctgttgatct
     1201 gtgtgacgct gagcaggtag tcgatgctcg tgcgggcgac gagttgcagg tagcgtcccc
     1261 tgcttggatg cggggagcat cctgccggga tccgctacat cggtcagtgg aaaacctcgg
     1321 tgcgaggcgc taatgtagca gagcagcgaa aatcggtcaa acccattgac ctcaagcgcg
     1381 tgtgagtcat ggagggacga gtgatgtacg atgtgaggat gaagcggcat gcggtgagca
     1441 tcatcggcac gacactgtct ttgtcgctag cattcgcgag cggatgcgag accaatccct
     1501 acacaggccg atcacaatta ctgatgacct cagtgtcgca ggaaatgcag ctgggtgcgc
     1561 aggcatacaa ccaggtaaaa aacgatccca aaatgaaacc atcacaggat tctagggaga
     1621 tcgagccggt gaagcgcgtg gctgctcgca tcgtcgaggc ggcgaaacga tcgaagtacg
     1681 cagagatggc gcagcagttt caatgggaag tcacggtgat caaggacgac aagaccgcca
     1741 atgcgtttgc gctgcccggc ggaaaaatgg cggtgtacac gggtatcttt ccgatggcca
     1801 agacggaagc cggccttgct gcggtcatgg ggcatgaagt ggtgcatgcc ttggcccggc
     1861 atggggcgga acggatgagt cagggccaac tcaccagtgc ggcgcttaaa gcggctggcg
     1921 cggcagcggg tgctagcggc atgaatccgc tcctttccca ggggacgatg gcggcactgg
     1981 gagcaggagc gcaagtcggc gtgttgcttc ctttcagtcg gaaacacgaa tcggaagcgg
     2041 attatatcgg cattctgctt gcggccgacg ccggatacga tccgcgcgaa tcggtggctc
     2101 tatgggaacg gatggcccaa atgtccggag gtggcgcacc agcggagttt atgtccaccc
     2161 atcccagtca tgaaacacgc atcgaccagc tgaagaaatg gcttccggaa gcgatgacta
     2221 tctatcagtc aaagcaaccg gttccggctg cggagttgcc accggttggc ccgtaacttt
     2281 acgggttaga ctatcgacga ggctctttct tgcatcacgc tcgttcattt cctatactca
     2341 atctgcgcga gggagaggac cgggtgatcg ctcgtggcgg tgacgccgcg ggaggaaagt
     2401 ccggacttca tgggcagagc gctgggtaac gcccaggcgg agcgatccga caccggcgcc
     2461 acagaaaaca aaccgccgat ggccaaagcg acgggacccc aatcgaatcg ctgcggatca
     2521 ggtaagggtg aaacggtgga gtaagagccc accgcgggga tggcgacatc accggcacgg
     2581 taagcgtcgc tcgatgcaag accaaatagg aagacagctg tcgctttctt ggaagcggcg
     2641 accggctggc ccggccgagg tcttcgggta ggtcgcttga ggttcgaggc aactcggatc
     2701 ccagagaaat gatgaccacc gttgaggaac agatccttga cgggacagaa tccggcttat
     2761 aggtcttctc caccgcgctt tcttaccttc gtccagattt cttactcttc atagccacgt
     2821 cggctgtgta gaagcctgtc tattgacctg gcactatcgt ggtgctagga tctttgatct
     2881 ttgtcattga tttttcagat aattctggat gatcagcggt aatggtgtat ccaccgatgg
     2941 cctgaatcga atgggtcaat ctgtgaatgg ccacgggagg gattggaggt tcgcatgaaa
     3001 ctgacaggcg ccgaaatttt tatagagtgc ctcaagcgtg aaggagtgaa aacccttttc
     3061 gcgctaccgg gaggggttgt cctcaagatt ttcgacatgc tgcatcaaca gaaggacatt
     3121 gaagtggtgt tgactcgcca tgagcaaggt gccggccaca tggccgaagg gtacgccaag
     3181 gccaccggca aggcgggtgt gtgtctagtg acctcgggac ccggcatgac caatgtgatt
     3241 accgccttgg ctgatgccta catggattct gtccccttag tgtgctttag cggtcaggtg
     3301 ccgaccagct tgattggaaa cgatgccttt caagaggcgg acaatgtagg cctaagccgt
     3361 ccgtgcacga agtacaactt tctcgtaaaa gacgtgaacg agctggcctc aacgatcaag
     3421 gaggcgttct acattgcaac caccgggcgt cctgggccgg tcttagtaga cattcccaaa
     3481 gacgtctcaa tggacaaagc ggagttcaat tatccgaatt ccgtgtcgat ccggggatac
     3541 aacccgactt atgaaggcaa taagtggcag gtcaaacagg ccgcagaagc catcatgaag
     3601 tcgaagaagc cgattctcta cgtcggcggc ggcgtgatct tctcgggtgc ctcgaaagag
     3661 ttaatcgaat tggccgagct cacgcagatc ccagtagaca tgacgttgat ggggctgggg
     3721 gctttcccgg gcgaacatcc tctgtcgtta ggaatgatgg gaatgcacgg cacataccag
     3781 gccaacatgg cagtgcacta ctcggatctc gtcgtggcaa tcggcgctcg ctttgatgat
     3841 cgggtgactg gcaaggtgtc cgagttctgt ccctacgcga aaatcatcca cattgatatt
     3901 gatccaacgt cgatcaggaa gaatattcac gtcgacattc caatcgtcgg tgactgcaag
     3961 gccgtgctgc gcgaattgaa tcagattctc cgggcgacag taaacggcga gcaaaaggaa
     4021 ctgcgtaaag catggtggga ccagattcgg gaatgggaga gcgcgcatcc cctcagctat
     4081 cagcaggagg cggacggccc catcaagccg cagcaagtca tcaagcgcct gtatgacttg
     4141 acgaaggacc tggatccaat cgtgtccacg gacgtcgggc agcaccagat gtgggcggct
     4201 cagtatttca agatggccaa gccgaaccgc tggttgacct ccggcggact cgggacgatg
     4261 ggattcggat ttcccgcggc aatgggtgcc caagcggcct ttcccggccg gctcgtcctc
     4321 tgcatcgcag gtgacggcag tgtgcagatg aacatgcagg aaatggcgac cgcggtcgtt
     4381 cacaaactcc cggtgaaaat catcgtctta aacaatcgat ttcacggaat ggtccggcaa
     4441 tggcaggacc tcttctacga gggtcgatat gcctcgagtt atctggacac cacaccggat
     4501 ttcgtcaagc tggccgatgc gtacggcgcc atcggtttgc gggcgtcaaa ggtcagcgaa
     4561 ttggacaacg tgctcaagga agccattgcg gctaaagggc cggttatcgt ggatgtgccc
     4621 acacatccgt acgaaaacgt ctaccccatg atcccggcag gcgggtgcaa tcacgagatg
     4681 attttggaag atacgccgga gctgaaactg aaacaaagcg gcggcgccaa ggtgactccg
     4741 caggacaaag acacgatctt gaccgcatag aggatcttgg gccggttgcc gagatccggc
     4801 agtggcattc ccggcaccga cgactcagag cagaacgatg gaacacatca tcttagtaac
     4861 ggtcgagaat aagtttgggg tgctgtcgcg gatcgccggg ttgttcagcg gtcgcggttt
     4921 caacatcgaa agcctatctg tcgcgccgac gttggatcca tccatgtctc aaatgacgat
     4981 cgtcacctcc ggtgatgagc ggatcgtcga gcagatcgtc aagcaactca ataagttgat
     5041 tgacgtcatc aaagtcgtgg atttgaacga aagcgatttt gtctcgcgtg agacggctct
     5101 gatcaaagtg cataccaggc aggaagaccg cgccgaagcc ttacgcatcg ccgatatctt
     5161 tcgcgcgaat gtcatcgatt cgacgcccgc gacctataca attgaagtca cgggtgatcc
     5221 gaaaaagatc gaagcgatca tcaatctcct gcagccgctg ggaatcaaag aattgaccag
     5281 gaccggccgg gtggccgttg cccgggaacc gctccgttcc acgacgacgc aacccaagaa
     5341 agtcgcccgc gagtaatgag gcgggtcccc cgcctgcctt tacgcatcaa ccttttgcct
     5401 tcgggagtcc atgatgaaga tttactatga cagggatgcc gatcttcaac agattaccaa
     5461 caagaaagtg gcggtcatcg gcttcggcag ccaaggccat gcccacgccc tcaacatgaa
     5521 agaaagcgga gtctcggtcg tggtcggctt gcgtgaaggc agttcgtggc gcaaagccga
     5581 gcagagcggg ctcaaggtca tgcccgtagc cgatgcggtg aaagcgtctg acgtcgtcat
     5641 gatcttggcg cctgacgagg cacaggctgc cctctatcgg caggaggtcg ctccgaactt
     5701 gaaggctggt tcgtacctag cgttcgggca tggattcaac atccatttcg gacagatcgt
     5761 cgccccgtcg acaatcaatg tgttcatggt cgctcccaaa gggcccggtc atttggtacg
     5821 atctgagtat accaagggca gcggagtccc ctgtctattg gcggtgcatc aggatcccag
     5881 cggcacaacc aagcaggtgg gtctggccta tgcgagtgcc attggcggag ggcgcgccgg
     5941 cgtcattgaa accaattttc gcgaagaaac cgagacggac ttgttcggcg aacaagccgt
     6001 cctctgtggc ggcctgacct cgttgataca ggccggctac gagactctcg tggaagccgg
     6061 ctattcgccc gagatggcgt attttgaatg tttgcacgag gtgaagctca tcgttgattt
     6121 gatctatcag ggcgggatcg caaatatgcg gtattcaatc agtacgacgg caaaatacgg
     6181 tgatgtgaca agaggcccac gcatcgtcac cgagcagacg aaacaggaga tgaaaaagat
     6241 cctcggcgaa atccagcagg gacagtttgc caaggaatgg gtgttggaaa atcaggcgaa
     6301 tcgtcctgtg tacaatgccc tgctggccaa aggtgaggcg catccgatcg aagaagtcgg
     6361 tgccaagctg cgagcgatga tgccctggct caagaaggat cagttggtcg ataagtcaaa
     6421 aaattgatgg aggggtaagg tctgtggcgg atcgtgcagc cggtgttccc ttcgccaaag
     6481 aaggaattcc cttcatcggc gctgccgccg gcattacact gatcgcatgg tggctcgatt
     6541 gggtacccgt ggcggtcgtg ggggccgtgc tgaccctctt cgtgtcctgg ttcttccgca
     6601 acccgccgcg acgggttccc caaggacctg ggctcgtcgt cgctccgggt gatggggtcg
     6661 ttcttgcggt cgaagaagaa ttcgagccgc ggtacctgaa ggatcgcgca gttcggattt
     6721 ctattttctt gaacgtgttc gacgtgcaca tcaaccggat cccgtgtgaa ggcatcgtcg
     6781 agaatgttca gtaccagccc ggcctgttcc tggtggccag taagcctcat gcgac
//
LOCUS       DASTQY010000167         4985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_439656, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000167 DASTQY010000000
VERSION     DASTQY010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439656"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..556)
                     /locus_tag="VFR82_05430"
     CDS             complement(164..556)
                     /locus_tag="VFR82_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4501073.1"
                     /translation="MTAIRILLVDDECLVRKILRQILATYPDVEVVGEAAAGDEAISS
                     VERLQPHIVIMDIRMPRMDGIAAAREISHKFSHVKIIGLTEYAYGYHADAMEKAGAAG
                     VYEKSKATEELYAAIKKISGQDSSIAWA"
     gene            1909..2400
                     /locus_tag="VFR82_05435"
     CDS             1909..2400
                     /locus_tag="VFR82_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4501074.1"
                     /translation="MGKATALQILLVDDNADIREALCSILRDCPDVRVVGEATNGEEA
                     VFTAEKLQPDVVLMDVNIPTLDGIAATRAIKAKHSNIAVVGLSANTPDYMVYAMVKAG
                     AYAVMPKENAVEDLYDLIRKAVSSIPRDLAAEAGAAPPAYCQGETSVNGSAGESSREL
                     PAK"
     gene            2748..2936
                     /locus_tag="VFR82_05440"
     CDS             2748..2936
                     /locus_tag="VFR82_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501075.1"
                     /translation="MPISKQILKLYGQYSIELAHRLKEEAPLNTEEQVFIENHLLIVQ
                     LALAMSKHVRPVSMQGEG"
     gene            2933..3529
                     /locus_tag="VFR82_05445"
     CDS             2933..3529
                     /locus_tag="VFR82_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4501076.1"
                     /translation="MKQALVVDDHPIVRDGIKELLQRAFPSVVIKDSSGTDGVLQEIC
                     AQPWAFVVLDINLPGQNGLDIIKKTKARQPDIPIIVFSLYSERQYAARALRAGAVAYL
                     SKERPPRDLIETVKTALEGGPIKKPREVLVSQPVLSDREVQVLALFVRGMSRKEISQE
                     LNINEKTVSTYRARLLHKLDVKNLVELIRYAVEEGLVD"
     gene            complement(3499..4254)
                     /locus_tag="VFR82_05450"
     CDS             complement(3499..4254)
                     /locus_tag="VFR82_05450"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEU4501077.1"
                     /translation="MIESSFCFLPGIGVKTERALWQQGLLSWAGFVSARAICGIGAVR
                     KTRYDREVLRAIERHAGGDARYFGVALPSRDHWRLYEWLRTGAVYLDIETDAFGRITV
                     VGLYSRKGMRSFVRGESLDGVALSEELRRYDLLVTFCGTAFDVPRLLNQFPDLPLDHP
                     HMDLCAIGRRLGYGGGLKAIERLFGINRPAEVSGLNGADAVRLWNRWRHRRDHHALAG
                     LLAYNQADCVNLEPLADAFYCRMVQSTSPSSTA"
     gene            complement(4315..4842)
                     /locus_tag="VFR82_05455"
     CDS             complement(4315..4842)
                     /locus_tag="VFR82_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HEU4501078.1"
                     /translation="MRTRCIELFGAVALTILAGCAEMPGGGTQTGASKPTPAQGYAIH
                     VMAPHKFEDGTVHGPYHHYCKPVSPEVIQCLLFESTDPNALLTDIEYFVAKSVTRAHV
                     PLEIWNKYYHDHEVEIGTGRVQVLDMPEAQAKEIAALAAKTDGIIFHLWPEGAKAPDG
                     TVGHPQSVGHKLRTQ"
BASE COUNT         1201 a         1345 c         1276 g         1163 t
ORIGIN      
        1 atgatactca catgtgtcta gttgttgttg agacctgagc gaatatcagg tttactagcg
       61 gtcgaacaac tgagggaggg tctgcttggc ccagcagcgc gaggtttgaa gtgtaccggt
      121 gacgatggct tgtggccgac acagagaaaa tccgacctct tactcaagcc catgcaatcg
      181 aggaatcttg cccactaatc ttcttgatgg ctgcatagag ttcttcagtt gcctttgatt
      241 tttcgtagac gcctgccgcc ccagctttct ccatcgcatc agcgtgatac ccgtatgcat
      301 actctgtcaa cccgataatt ttcacgtgcg aaaacttgtg actaatctcg cgcgctgccg
      361 cgatcccgtc cattctcggc attcgaatat ccataatcac aatgtgcggt tggagtcttt
      421 ccacgctgga aatggcttcg tcacctgctg ctgcctcccc tacgacttcc acgtccgggt
      481 atgtagccag gatctgccta aggatcttgc ggaccaaaca ctcatcgtct acaagcagaa
      541 tgcgaatagc cgtcatcatt ctcacctcta aatacgagtg ggaactgaag gttggcggaa
      601 tgtagcatac atggcagtaa ctgctacagc aaaatcgatg tggtatgtag gcctacggtc
      661 ctgtaagccg caacctacga ggataagttg agggagtaac agggcagctt caaggccgaa
      721 tcagccgacc gcgttgcata atcatcgcgt ccgtccttag gcctctttat tctatgtttg
      781 cccgccgtgt tctttcggct gtgtatgacc ctcaccaatt gagctgacag cgctgtttgt
      841 ggctacgagc cccgcggaag ccacaagcga atctttgttt cacctgcctc aattccgccg
      901 aacaagtgac ttcaatagcc atcccctagt gcaccgtatg cgatgacagg ctcagctgat
      961 ccggagaata agcgatatac aaacaaagac ggatgctttc ggatcgtcta agaatcgtcc
     1021 ctgtccgttt gcggattgaa ataaatgctc aacaaaatgt gggatggaaa ctagggtttc
     1081 acctagatgt cacattgaag aagattcatt acgttcgcac tgtgttcatc gaaaggggga
     1141 gatcatggaa gtgcgctggg ataccacgca tcaagagagc aatccgcctg ctccgtccca
     1201 aaataaggtt gaccgccaga ttcaagacga acgcgatcct aggctcactt tagaaatggc
     1261 tattgagtgg gctcacctcg gtggggacta agcgagcagt ttcgtcagcc gctcgacctc
     1321 tctcgttcac atcgcacatt agttccccct ctcgccgagt ggaatctgca tgtcagcaga
     1381 gactcttgta ccgcggggcc tcacgttttt acccttaacg gtcacgcaca acggaatctt
     1441 gaatattttg tgaccgaagg cctacagctc ctgcgttgac atcggctgtc gaaggcaaca
     1501 tactacgcac gctttttcct atctctcggg gcaacgccac cagacggatc ctctatgcag
     1561 gtggccagcg aagtgaaaga cttctcgggc gcttgtgaac gcctcctatc gacgatcgca
     1621 atgaaccgac aattgaccga ggaggaagcg aagatgattc aatactattg caaagaactt
     1681 ctcgctaagg ttgcaccgcg tttacatgaa cgctgaatga cgttcccact ttagctttca
     1741 ggatctctta ttggcggcca gagcattcgt cgcttcttga taattcggat ggtgaagtat
     1801 aacacttcac catcttcaaa ggttgggccc cttactcact cgttgcggat tgtcatccga
     1861 cgtttcattc tgttatatga tgaattctcc tatttcagga aaggtcttat ggggaaagcc
     1921 accgcactgc agatcctctt ggtggatgac aatgcggata ttcgtgaggc cctgtgctcg
     1981 attttgcgcg actgtccaga tgtccgagtc gtcggcgaag cgactaatgg tgaggaagcg
     2041 gtctttaccg ctgagaaact gcaacctgac gttgtgctga tggacgtaaa tattcctacg
     2101 ctggacggca ttgcagcgac acgtgccata aaagcaaaac attcgaacat tgccgtggtt
     2161 gggctttctg caaatacgcc tgactacatg gtctatgcca tggtcaaggc aggggcctat
     2221 gcagtgatgc cgaaagaaaa cgccgtggaa gatctgtacg atttgattcg gaaagcggtg
     2281 tcatccatac ctcgcgacct cgccgctgaa gctggtgctg ccccgcccgc ttactgccag
     2341 ggtgagacgt cggtcaatgg atcagcagga gaatcaagtc gcgagctgcc cgccaaataa
     2401 acgcaaacta cgttgatcag tcagacccct ccactccttt gcttcccagg acgagtacga
     2461 gggacgttgt taagacatcg gataaaacgc gccccctctg cctacaagct tgtaggcact
     2521 cgctgataga caagtggact tcaaaagact caatctttca tattcacaag cgagttagcc
     2581 ctcaaggctc ctacttcttc tttcttatca gcgcagaacg atccgtaaag gcaatgtcag
     2641 aaactaacca tgtctggaat tgcacgacgc ccctaggctc ttgctgatag acaaactaag
     2701 cgtttaatct atattccgct ggtacgttca acacgtgcag gtttgccatg ccaatcagca
     2761 agcagatatt aaaactttac ggacagtata gcatcgagct ggcgcaccgg ttgaaggagg
     2821 aggctccgtt aaacaccgag gaacaggtgt ttattgaaaa tcatctcttg atcgttcaac
     2881 tcgcgctggc catgtcgaaa catgtcagac cggtgtcgat gcagggagaa ggatgaaaca
     2941 agcgcttgtg gtcgacgacc accccatcgt gcgtgatggg atcaaggaat tgcttcaaag
     3001 ggcgtttcca tcggtcgtca tcaaagattc ttcagggacc gatggcgtgc tgcaagaaat
     3061 ctgcgcgcaa ccgtgggcgt tcgtcgtcct cgatatcaat ctgcctgggc aaaacggtct
     3121 ggacatcatc aagaaaacga aagctcgcca acccgatatt cccatcatcg tcttcagctt
     3181 gtactcggaa cgacaatatg cggcccgtgc gcttcgggcc ggggccgtcg cctatctctc
     3241 gaaggaacgc ccgccgcgtg atttgatcga gacggtgaag acggcgcttg aagggggacc
     3301 gatcaaaaag cctcgggaag tcctggtgtc tcagccggtg ctctcggacc gcgaggttca
     3361 ggtcttggct ctctttgtca gagggatgag tcgaaaggaa atttctcaag agctcaacat
     3421 caatgaaaaa acagtcagca cctatcgtgc aaggcttttg cacaaattag atgtgaaaaa
     3481 cctcgttgaa ctgatccgct acgcggttga agaggggctc gtggactgaa ccatccggca
     3541 atagaaggca tcggccagcg gttcaagatt gacgcaatcc gcttgattgt aggcgagaag
     3601 cccggcgagg gcgtggtgat cccggcgatg ccgccagcga ttccagagac gcactgcgtc
     3661 tgcaccattc aaaccagaga cctccgccgg tcggttgatg ccgaagagcc gttcaatagc
     3721 tttaagacct cctccgtacc ctaaccgtcg tccaatcgcg catagatcca tatggggatg
     3781 gtcaagcgga agatctggga actgattcaa tagcctaggg acatcgaagg ccgttccaca
     3841 aaacgtcacg agcaaatcgt atcgacgcaa ctcttccgaa agtgcgacac cgtccaacga
     3901 ttcccctcgg acgaacgagc gcattccctt tcgactgtaa agtcccacca cggtgatccg
     3961 accaaacgca tccgtctcga tgtcaagata gacagctcca gttcgaagcc attcataaag
     4021 acgccagtgg tcccgggacg gcagggccac gccgaagtag cgggcatcgc cgccggcatg
     4081 ccgctcgatc gctctcaaga cttcccggtc gtagcgtgtc ttgcggacag ctcctatccc
     4141 gcagatcgca cgtgccgaca cgaacccggc ccatgacagc agaccctgtt gccagagagc
     4201 tcgttccgtt ttgaccccta tgcctgggag gaaacaaaaa gaagactcaa tcactatggt
     4261 ctcatttgct gcagacgttt cattggtgga acaaccgact gcaaagtttt ggccttattg
     4321 ggttctcagt ttatggccga ccgactgcgg atgtcccacc gtcccgtccg gagccttggc
     4381 accttcgggc caaagatgga agataattcc atcggttttg gccgcgagcg ctgcaatttc
     4441 ctttgcctgt gcttcgggca tgtccagcac ctggacacga ccagtcccga tctccacctc
     4501 atgatcgtgg tagtacttgt tccatatctc caggggtaca tgggcacgtg tgactgattt
     4561 tgccacgaaa tattcaatgt cggtcaagag tgcgtttgga tcagtcgact caaacaacag
     4621 gcattgaata acttccggag agacgggctt gcagtagtga tggtaaggtc cgtggactgt
     4681 gccatcctcg aatttatgag gcgccatcac atgaatggcg taaccctggg ccggtgtagg
     4741 tttgctggct ccagtttgag tgccaccgcc cggcatctcc gcacagccgg ctaggatggt
     4801 cagcgccacc gctccaaaga gttcaatgca tcgtgttcgc atgtcaagcc tccagtcttg
     4861 aagtaacaca caacgatagg gcagctcacc attttggagt gagcgggcac tccctgaacc
     4921 ctcacctact tgggcaattt gggcactgtg actttcagcg gtgctccggt caaggccttc
     4981 agaaa
//
LOCUS       DASTQY010000168         3702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_14293, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000168 DASTQY010000000
VERSION     DASTQY010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14293"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1934
                     /locus_tag="VFR82_05460"
     CDS             <1..1934
                     /locus_tag="VFR82_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU4501079.1"
                     /translation="IVYLTFCLLLWSSCEPIATKTYQVRQRAFTYTPSSLIQDYQEEY
                     TYIEQRRRAVMSADRDIDGDTFPRAEKVPSHLSGLAFSGGGIRSATFHLGILQALQDM
                     GSLPQIDYLSTVSGGSYIAGWMLAHLGQELDDPYGNLVQTPDQATLLDPHGDFVTHLR
                     HHAGFIREGGFWEGPKLIWGYLWRLIPYYVWDLVLHIKTPPDIGNELHLFTPYQHRIE
                     VTYLRGKHETPLVRLNRKDVDAPYLIINGNLVNRGRPRGYETESDQPYRDNYNFEFTR
                     DYTGSDGLGYVQSAGFGLPVVEAQTQDGSPASFNPRQVVVQDLTEGTCHKTWGERIRP
                     HACVRLSQAMTASGAGLDLDSLVQEWYRDDIARLTLGILTAPFNLNNEYQTWNFARRH
                     NTTLGTIWDYVLMLSIQRIWPDTKSRWIEITDGSFYDNLGAQTLLRRQVAHLIVGDAT
                     LDTTWQYDYLYNLQARIKGYFGPGVEWCGEIPPKNEIVWYRRFWVIRPDGVPTVLHYL
                     KPYAYNPILFRQNPSLLAPGKIYVSPEPESGRSLVTHPLTSPPVNPTHLDANWLLPLV
                     TDRASYEKVKLALKKVSKFVDSPTGEEFPQTSTIFQWYKLEEFEAYRQLGYLMGWAYL
                     STVTYSADELAPAASCKTL"
     gene            complement(1954..2322)
                     /locus_tag="VFR82_05465"
     CDS             complement(1954..2322)
                     /locus_tag="VFR82_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEU4501080.1"
                     /translation="MPPRSLPPCPSSPNCVSTLASHADTQHAIAPYRYQKSLAETKDL
                     LKATIASLARTRLVEEDGEYLHYEFTSLLIRFVDDVEFVFDEGSKTIHFRSASRVGYG
                     DLGVNRRRMENIRSYLTGRI"
     gene            complement(2423..>3702)
                     /locus_tag="VFR82_05470"
     CDS             complement(2423..>3702)
                     /locus_tag="VFR82_05470"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HEU4501081.1"
                     /translation="VAGTTDRRAARLRAASCLRLTRLLKQRQLRAQVFSSDPIPSHIF
                     VAQRHRIPHSRHMRPLSRVQSLLAKPFMPAVFFLSGVTYDTLTLTRIDRLLDNLVLLL
                     YLLLLGLLIVLTGRLGIEPSPDPAQLKTLSPVARWVLQTRPFYPMASQFLLGGLFSAY
                     AIFYFRSATLTSTAVFFSLLVLFLVANEFLRDRLSNLRLLVSLYALVCFGFFTFFLPV
                     MIGYMNVLVFLGGAVLSGAVTFRVVQLIYRNNPDRSRREAVGVGVPAVAVIVLLVGFY
                     FLNWIPPVPLSMTFGGIYHEVKKSDDRFELSFERSWYQVWKQSNNTFPADEPVYCFTA
                     VFAPVALNATVYHHWSFRPSRELPFRHADRIPVRISGGREGGYRAYTFKQRLDPGDWR
                     IDVETEDGRIIGRVSVRVEMRAEPQPPLTTIVY"
BASE COUNT          886 a         1056 c         1000 g          760 t
ORIGIN      
        1 cgatagtcta tttgacgttc tgcctcctgc tttggtcgtc gtgtgaacct atcgccacca
       61 aaacgtatca agtccgccaa cgagctttta cctatacacc gtcctctctg atccaggatt
      121 accaggaaga atacacctac atcgaacagc gacggcgagc agtcatgagc gccgacaggg
      181 acatagatgg agacaccttc cctcgagcgg aaaaagttcc ttcgcacctc agcggattgg
      241 ctttctcagg cggcggaatt cgttcagcaa cctttcattt gggtatccta caggcgctcc
      301 aagacatggg cagtttgccg caaattgact atctcagcac agtctcaggc gggtcttata
      361 tcgccgggtg gatgctcgcc cacttggggc aggagcttga cgatccttat ggcaatcttg
      421 tccaaacacc ggatcaagcc acgctactcg accctcatgg cgattttgtt acccacctgc
      481 gtcaccatgc cggctttatc cgagaaggcg ggttttggga aggccccaaa ctgatttggg
      541 gctatctgtg gcggctgatc ccctactatg tctgggatct tgtcttacat atcaagacac
      601 cgcccgatat cggtaacgag ctgcatctct tcacccctta tcaacaccga atcgaggtga
      661 cctatctgcg cggcaaacac gagactcctc tggttcggtt gaaccggaag gacgtcgatg
      721 ccccttacct catcatcaat gggaatcttg tcaatcgcgg tcgtccaagg ggctatgaga
      781 ccgaatcgga ccaaccgtat cgcgacaatt acaactttga attcacgcgc gactataccg
      841 gatccgatgg acttggctat gtccaaagtg ccgggttcgg tttaccggtc gtggaagcgc
      901 aaacccagga cggcagcccc gcctcattca atccccgcca ggtcgttgta caggacctta
      961 cggagggaac ctgccacaaa acatggggag aacggatcag gccgcacgcc tgtgtgcgtt
     1021 tgtcacaagc catgaccgca tcaggcgcgg gactcgatct cgacagcctc gtccaggaat
     1081 ggtatcgcga cgatatcgcc cgtttgacac tgggtatcct cactgcaccc ttcaacctga
     1141 acaacgaata tcagacgtgg aattttgcac gccggcataa cacgactttg ggcaccatct
     1201 gggactacgt gttgatgctg agcattcaac gcatctggcc ggacaccaaa agccgatgga
     1261 tcgaaatcac cgacggctcg ttttacgaca atctgggcgc ccagaccttg ttgcgtcgcc
     1321 aggtggcgca tctcatcgtg ggagacgcca ccctggatac gacctggcaa tatgactact
     1381 tgtacaacct tcaagcgcgc atcaagggct attttggccc gggagtggag tggtgcggtg
     1441 aaatcccgcc gaagaatgag attgtctggt accgccgatt ttgggtgatc cgcccggacg
     1501 gtgttccgac cgtgcttcac tatctaaaac cttatgccta caatcccatc ttgttcagac
     1561 aaaacccgtc gcttcttgcc ccggggaaga tttacgtgag ccccgaacct gagagcggtc
     1621 gttccctcgt cactcatccc ttaaccagcc cgccggtcaa cccgactcat ttggacgcaa
     1681 attggctcct tccgctcgtg acggaccggg cctcatatga gaaggtgaag ctggcgctca
     1741 agaaagtgag caaattcgtc gactccccca ccggcgaaga attccctcag acgagcacga
     1801 tattccagtg gtacaaactc gaagaattcg aagcctaccg ccagctcggc tatctcatgg
     1861 gttgggcgta tctctcgaca gtcacctatt cggccgacga acttgccccg gcggcctcct
     1921 gcaaaacatt gtgaggggac agcttcctcg tttctaaatc cgtcccgtga ggtacgaccg
     1981 gatgttctcc atccggcggc gattcacgcc caggtcgcca tagccgaccc gggaggcgga
     2041 ccggaagtga atcgtcttgc tgccttcgtc gaaaacaaat tccacatcat cgacgaaccg
     2101 gatgagcaag ctggtgaatt catagtggag gtactcccca tcctcctcga ccagtctggt
     2161 acgggccagg ctcgcgatgg ttgccttcag aaggtctttc gtctctgcca gtgatttctg
     2221 atagcggtag ggcgcgatcg catgctgagt atcagcgtgg gacgcaagcg tcgacacaca
     2281 attgggactc gaaggacagg gcggcaatga acgtggcggc attgatgagg gactctacgc
     2341 ccttggatac cctgcggcaa ccccccattt ctctgtgaac tgctcactca ctcgatgaca
     2401 cggctgaata ggaccttgca tctcaataga caatggtcgt caagggcggc tgcggctcgg
     2461 ccctcatctc aacacgcacc gagactcgtc caatgattcg cccatcctcg gtttccacat
     2521 cgatacgcca gtccccagga tcgagacgtt gcttgaaggt atatgctcga tagccgcctt
     2581 cccgaccgcc ggatatccgg acaggaattc tatcggcatg tctaaacggt agctcccggc
     2641 ttggacggaa agaccaatga tggtagacag tggcattcag tgcgaccggc gcgaacacgg
     2701 ccgtgaagca atacaccggc tcgtcagcag gaaacgtgtt gttcgattgc ttccagactt
     2761 gataccaact tcgctcaaat gacagttcaa accgatcgtc tgattttttg acttcatggt
     2821 aaatcccgcc gaaggtcatg gagagcggca cgggcggaat ccagttcaga aaataaaatc
     2881 ccactaatag cacaatgacg gcaacggcgg gcacgcccac gccgacggcc tcgcgcctgg
     2941 atcgatctgg attgttccga tagatgagct gcacgacgcg aaacgtgact gcgccgctca
     3001 agacagcgcc tccgagaaag accaacacgt tcatatagcc gatcatcacg ggtaagaaaa
     3061 atgtgaaaaa tccgaagcag acgagcgcgt ataagcttac cagtaagcga aggttggaca
     3121 ggcggtctcg tagaaactcg ttggccacca gaaaaagcac caacaggctg aaaaagacag
     3181 ccgtactggt taaggtggcg ctgcggaaat agaagatggc gtacgcgctg aatagtccgc
     3241 ccagaagaaa ctggctggcc atcggataga aaggccgcgt ttggagcacc caccgtgcga
     3301 cgggggaaag cgtcttgagc tgcgcaggat ccggcgatgg ctcaatgccc agccgcccgg
     3361 tgaggacaat caggagtccc agaagcaaca gatagagaag cagcaccaag ttgtcgagga
     3421 gccgatcgat ccgcgttaag gtgagggtgt cataggtcac gccggacagg aaaaatacgg
     3481 cgggcatgaa cggcttggcc agcagtgact ggaccctgga aagcgggcgc atgtgtctac
     3541 tgtgcggaat ccgatgcctt tgagcaacga aaatgtgact tggaatagga tcagaggaga
     3601 agacctgagc acgaagctga cgttgtttca gcagtctcgt cagacgaagg caggaagcgg
     3661 cgcgtagacg agcggctctc cgatcagtcg ttccagccac cc
//
LOCUS       DASTQY010000169         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_639931, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000169 DASTQY010000000
VERSION     DASTQY010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639931"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1200
                     /locus_tag="VFR82_05475"
     CDS             124..1200
                     /locus_tag="VFR82_05475"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4501082.1"
                     /translation="MKPQYRLQRTITARMRRLIILLHCFVFVLTAGMLAEHAHAFKIT
                     EPAAGTKLGAGQTVTAHVDLGNDTGIIKVRYYWYGEQDDTLVEQDDATAMGSIIAPVA
                     LVGLSDHDPAFGGKLVVPKEGIGPMRLLAVAEISRGRLGSRSVFDEILIQVEPKAALT
                     TIDFETDKPLQLGRSGQSSAFGHVDSMGKIFELPVVGDFADGVTRRISAPSTGTSYDS
                     SNPKVIKVLPSGLLQIVGNGKTIITVKNRDKQAQLDVDVNVNEEPNEPPVADAGGPKT
                     VKAGTKVKLNGLRSQDAEGEALYYAWSQVRGSKVALLDVNGPEATFQAPQVSEARTYR
                     FKLRVTDKKGADSVPAFVDVTVEP"
     gene            complement(1297..>2188)
                     /locus_tag="VFR82_05480"
     CDS             complement(1297..>2188)
                     /locus_tag="VFR82_05480"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEU4501083.1"
                     /translation="ENVPLGTLVPVSTRYNLFLNGGSMSAEGSVEYAPTAKLVNLKRL
                     RIDGLHADYVHAKQTQRAEEARARATVSTAQQVHAKEDALVRVEEITIVNSEFGFVNQ
                     AAQPEYRVFLADAEASLQHYSNRLQEGAASMQLRGKFMGNGETSVTGILRQEHESPDF
                     ELRVKIAGTPIRAMNNLLRAHGKFDTVGGTFSCYSEVLLNDGRIRGYVKPLVRKLNVY
                     DEAQDRKKNPLEKVREGAIEDLSTLLENVPRDEVATKVDMSGSAHRPRTEIVQIVIGL
                     IQNAFFKAILPGFEQEFRSK"
BASE COUNT          472 a          634 c          606 g          476 t
ORIGIN      
        1 tctgcccgcc cgctggtggc aaaacatctt gcataatcaa cgggccctcc tgctgaaagg
       61 tgccctgttg ttcaaggatc gcgtcgtctt aacggatgtc cccttccacc tgacgaggtg
      121 accatgaagc cacagtatcg attacaacgg acgattactg ctcggatgcg aaggctgatc
      181 atcctgctcc attgtttcgt gttcgtgctg accgccggta tgctcgcgga acacgcccat
      241 gcgttcaaaa taactgaacc ggcggcaggg accaaattgg gagcgggaca aaccgtcacg
      301 gctcacgtcg atctcggaaa cgacacgggc atcatcaaag tccgctatta ttggtatgga
      361 gagcaggacg acacactcgt cgaacaagat gatgcgaccg cgatgggatc gatcatcgct
      421 ccggttgcgc tggtcggtct gtcggatcac gatccggcgt tcgggggcaa attggtcgtc
      481 ccgaaagagg ggatcggccc aatgcgactc cttgccgtag cggaaatctc acggggacga
      541 ctgggctcgc gctccgtgtt cgacgaaatt ctgatccagg tcgagccgaa ggccgcgctg
      601 acgacgatcg atttcgaaac ggacaaaccg ctgcagctcg gacgttccgg gcagtcgtcg
      661 gccttcggac acgtggattc gatgggcaaa atcttcgagc ttcccgtggt cggcgacttt
      721 gccgacggag tcacccggcg catcagcgcg ccctcgacgg ggacaagtta cgattcctcg
      781 aacccgaagg tcatcaaagt gctgccaagc gggctgctgc agattgtggg caatgggaaa
      841 accatcatca ccgtgaagaa tcgtgacaag caggcgcaac tggatgtgga cgtcaacgtc
      901 aatgaggagc cgaatgaacc accggtcgcc gacgccggtg gcccgaagac cgtcaaagcg
      961 ggaaccaagg tcaagttgaa cgggctcagg agccaggatg ccgaaggtga agcactgtat
     1021 tatgcctgga gccaggtacg cggaagtaaa gtcgcactgc tggacgtcaa tgggcccgaa
     1081 gcgacattcc aagctccgca agtgtccgaa gcaagaacct accggtttaa attgagagta
     1141 acggataaga agggcgctga tagcgtcccg gcatttgtgg atgtgacggt cgaaccgtaa
     1201 actgcccgcc tgggtgcggc ggccttgtag gggcaatctc ctggcgattc gtttctcccc
     1261 tctccactga cccgcctcat ttgacccgca tcattttcat ttcgaccgga attcctgctc
     1321 aaagcccgga aggatcgctt tgaagaacgc gttctggatg aggccgatca caatctggac
     1381 aatttctgtc cgcggcctgt gcgcactgcc ggacatatcg accttggtgg caacttcatc
     1441 acgagggaca ttctcaagca acgtagagag gtcttctatc gccccttcac gcaccttctc
     1501 gagggggttc ttcttgcgat cctgcgcttc gtcatagacg ttcaattttc tcactaacgg
     1561 cttcacatac ccgcgaatcc tcccgtcatt caacaagacc tcggaatagc aggaaaacgt
     1621 cccgcctaca gtgtcgaact ttccatgggc acgcaagaga ttattcattg cccggatcgg
     1681 ggtgcccgca attttcaccc ttaattcgaa gtccggggac tcatgctcct ggcgcagaat
     1741 accggtgaca cttgtctcgc cgtttcccat gaatttcccc cgcaactgca tggaagcggc
     1801 cccttcttgc aatcgattgc tgtaatgttg gaggctggcc tccgcatcgg ccaggaacac
     1861 gcggtattca ggctgggcgg cttgattgac aaacccgaac tcgctgttga cgatggtgat
     1921 ttcctctact cggacgagcg catcttcctt cgcatgaacc tgttgggccg tgctgaccgt
     1981 cgctctcgcc cttgcctctt ccgctcgttg cgtctgtttt gcatggacgt aatccgcatg
     2041 gagcccgtca atcctgagcc gcttcaagtt caccagcttt gcagtcggtg catattccac
     2101 ggacccttca gctgacatgc tgcctccatt gaggaaaagg ttgtatcgag tcgacaccgg
     2161 cacgagtgta cccaagggta cgttctct
//
LOCUS       DASTQY010000170         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_22444, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000170 DASTQY010000000
VERSION     DASTQY010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22444"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /gene="queA"
                     /locus_tag="VFR82_05485"
     CDS             <1..786
                     /gene="queA"
                     /locus_tag="VFR82_05485"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU4501084.1"
                     /translation="LFVKPVADREWEVLMKGTWRAGQAIEIDGESDVTVLSREGGRTV
                     VRINGAWPIPEWFRVHGCMPLPPYIKRLPTDQDREWYQTVFARDEGAIAAPTAGLHFT
                     PALLSQLTDRHIDLATITLHVGVGTFKPVTAECLEEHRMDAEWVEVGTTAVRAIERAK
                     AGGRKVVAVGTTVVRALETAALQGMLQPYRGETSLFVRPGFQFKVVDALLTNFHLPQT
                     TLLMLVSAFAGTGFLRRAYEEAVQTGYRFYSYGDAMLVTDRSS"
     gene            complement(783..1481)
                     /locus_tag="VFR82_05490"
     CDS             complement(783..1481)
                     /locus_tag="VFR82_05490"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEU4501085.1"
                     /translation="MIKILRFANEKYPWFLAITMGFIAVSFIVGMGWWGFGEQSGSVV
                     ATVGDLPISLDEFKRAYENTYRFYKEKVPGDFKDETIKQYVVEQLVENRVWLVAAHNM
                     GLTVADDELRESIMQIPEFQNNGKFDPERYQRLLAANHLTPSIFETMQARELLSVKAR
                     VLVSNAVSLTPSELAEAQALMLRQPDPDPGKAAAAKERTIQDVLFQKQQRALMAYAQS
                     LKATLPIAIHRELL"
     gene            1656..2111
                     /locus_tag="VFR82_05495"
     CDS             1656..2111
                     /locus_tag="VFR82_05495"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEU4501086.1"
                     /translation="MAEQVAFHPLGAGIYPKATVLNRFIAKLIDVFLAAAAAEVIVPV
                     GLLAGVAYLFVADGFAGGRSIGKRLIGLQTVLPDSREPAGFRESIIRNIPFAAAQVLF
                     VVPYVGWLASTVIVAFEAILIIGNEQGRRLGDELAGTQVLDAGRLALPE"
BASE COUNT          436 a          613 c          691 g          521 t
ORIGIN      
        1 cttttcgtga agccagtggc cgatcgtgag tgggaagtcc tgatgaaggg aacttggcgg
       61 gccgggcaag ccatcgaaat cgacggagaa tccgatgtca ccgttctctc gcgggagggc
      121 ggccgaacgg tcgtccggat caacggagcc tggcccatcc ccgagtggtt tcgggtacac
      181 ggctgcatgc cattgcctcc ttacatcaag aggctgccaa ccgatcagga tcgggagtgg
      241 taccaaaccg tgttcgcacg ggacgaaggg gccatcgctg cgcctaccgc cggcctgcat
      301 ttcactccgg ctctcttgag ccagttgacg gatcgacaca tcgatctggc gacgatcacc
      361 ctgcatgttg gcgtagggac cttcaagcca gtcaccgcag agtgcctcga agagcatcgc
      421 atggacgcgg aatgggtgga agtcggaaca accgccgtgc gcgcaatcga gcgcgcgaaa
      481 gcgggtggac gaaaggttgt ggccgtcggt accacggtgg ttcgtgccct tgaaacagcg
      541 gctctgcaag ggatgctgca gccgtatcga ggagaaacga gtctgttcgt gagaccagga
      601 tttcagttca aggtggtaga cgcgctcttg acgaatttcc atctgcctca gacgaccctc
      661 ctgatgttgg tctccgcgtt cgcaggcacg gggtttctac gtcgcgcgta tgaggaggcc
      721 gtgcagaccg gctaccgctt ttacagctat ggagatgcga tgttggtcac agaccggtcg
      781 tcttagagca gttcccggtg gatagcaatc ggaagggtcg ccttaagcga ttgggcatag
      841 gccatcagcg cgcgctgctg cttctgaaac agcacgtctt gaatggttcg ttcttttgcc
      901 gccgcggctt tgcccggatc gggatccggt tgtctgagca tgagggcttg tgcttccgcg
      961 agctcggacg gcgtcaagga gacggcgtta ctgacgagta cgcgggcctt cacgctcaac
     1021 agttctcttg cctgcattgt ctcaaagatt gagggcgtca aatggttggc tgccagcaat
     1081 cgttggtagc gttcaggatc aaatttgccg ttgttctgga actcaggaat ttgcatgatc
     1141 gactcgcgca gctcgtcatc ggccaccgtc aaacccatgt tgtgcgcggc gaccagccac
     1201 acccgattct ccaccaattg ctcgaccaca tattgcttga tcgtttcgtc cttaaagtct
     1261 cccggaacct tttccttgta aaagcgatag gtgttttcat atgcgcgttt gaattcatcc
     1321 aatgagatgg ggagatcgcc caccgttgcg acgaccgagc cggattgctc gccgaaaccc
     1381 caccagccca tgcccacgat gaacgacacg gcgatgaatc ccatcgtgat cgccaaaaac
     1441 cacggatatt tttcgttcgc gaaccgtaag atcttgatca tggaccggga tccctcccga
     1501 caagtgaaat agtcgcggat tgtacccagc ctcctgtgcc gaaggcaagt ttctcagtgc
     1561 ccatgggcct cgccgtcgtg gcgtcaaaga atggtgattc gtttgtgcta ggtcctggtc
     1621 tttgttatac tttgccccta attacgagga gatccttggc ggagcaggtg gcgttccatc
     1681 cattgggtgc agggatctac ccgaaggcga cggttctcaa tcgattcatc gcgaagctca
     1741 ttgacgtttt tctcgcggca gctgccgccg aagtcattgt gcctgtcggt ttgctcgcag
     1801 gagtcgccta tctcttcgtg gccgatggat ttgcgggcgg tcggagcatc ggtaagcggc
     1861 tgatcggcct tcagacggtt cttcctgata gccgtgaacc ggcaggattt cgagagtcca
     1921 tcattcgcaa tattcctttc gcggcggcgc aggtattgtt tgtcgtccct tatgtcggct
     1981 ggctagcctc gacggtgatt gtcgcgttcg aagcgatctt gatcattgga aacgagcagg
     2041 gccgccggct cggtgatgaa ctggcgggga cccaggtgtt ggacgcgggc cgattggcat
     2101 tgcccgagta ggaacaggcg catgtgaggc tggcgtgagc cgcgtcatcc aggattcggt
     2161 ttgcatgtga agggagagtg accgtgggtt ttacgagtga cgtgttcggc tggttctcga
     2221 acgacctggc catcgacctg ggcacagcca cgacgttggt g
//
LOCUS       DASTQY010000171         5459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1290142, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000171 DASTQY010000000
VERSION     DASTQY010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290142"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..552)
                     /gene="bfr"
                     /locus_tag="VFR82_05500"
     CDS             complement(76..552)
                     /gene="bfr"
                     /locus_tag="VFR82_05500"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEU4501087.1"
                     /translation="MKAKEGVVNLLNKILTADLTAINQYFVHGKMCENWGYERLYHKV
                     RERSIDEMKDADELIGHILYLEGLPNVQRMNTVHVGETVPEQLKLDLKAEQEMLALLN
                     DGVVHCAKVMDFTTRHMLEDMAKDVDEHIDWIETQMETIKQIGLENYLAEQIKKES"
     gene            complement(793..3132)
                     /gene="topA"
                     /locus_tag="VFR82_05505"
     CDS             complement(793..3132)
                     /gene="topA"
                     /locus_tag="VFR82_05505"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU4501088.1"
                     /translation="MAKSLIIVESPTKAKTITKYLGRGYTVMASVGHIKDLPTSKLGV
                     DLEHNFKPQYVTIKGKSKVLADIKKKAEEADKIFLAPDPDREGEAIAWHLSEELRGKS
                     KKKNDGRVFRVLFNEITERAIKQALQSPGQIDMKLVNAQQARRVLDRIVGYQGSQLLW
                     NKVRRGLSMGRVQSVAVRLMCEREAEREAFRAEEYWSIIVLLAGTNPPPFEAKLHSIN
                     GEEASIGNAEQAGQIVSAIEGKAFAVTSIERKEKKRNPVAPFITSRLQQEAARKLHFT
                     PKKTMTLAQQLYEGVEIGSEGSTGLITYMRTDSPRISQEAMADARQVIQERFGAEYLP
                     DVPNVYKTSKAAQEAHEAIRPTSAARDPESIRPYLEQDQYNLYKLIWNRFIASQMVPA
                     ILDVTRVESTPLETSARYVFRSSGTIVKFPGHTIVYMEGVDKELFAQKPKQEEEAEDT
                     AERQLPELTEGERLRLVEQAEQTIPGVTSKQHFTQPPPRYNEALLIKELEEKGIGRPS
                     TYAAIISTIQERKYVEKVDGRFLPTETGRTVNDFLLKGFPDVVNVDFTSQLEAELDEV
                     EEGNKPWVDAVRDFYDPFTADLEKAKNIPGPKDTVEPPTNIPCEKCGRMMEIKWGRNG
                     KFLACPGYKEDPPCKNTQNFEKLPDGTIKIVPKQELTTDQVCEKCGSPMLVKTGRFGK
                     FIACSAYPQCKTTKPLALGVKCPQPGCGGDLVQKRTRKGRSFFACSKYPACEYALWDR
                     PIPKACPTCSAPFLIEKVSKQAGRSVQCRNEDCGYREAG"
     gene            complement(3219..4352)
                     /gene="dprA"
                     /locus_tag="VFR82_05510"
     CDS             complement(3219..4352)
                     /gene="dprA"
                     /locus_tag="VFR82_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEU4501089.1"
                     /translation="MQSLAASKTGSLEAWMRLRTLEGIGDHTLLALVREWHSPDAVLA
                     APADELVQRGCSPRLADVIRRGPDREVCRRIARELSAIERKRISVRTILDSAYPSRLR
                     TIADPPPLLYVSGSLEESDQLAVAVVGARRATPAGRVIPEHLANELAASGFTIVSGLA
                     RGVDAAAHRGALTAGGRTVAVLGCGLGRTYPAEHERLRHEIEERGAVVSELPLDAPPH
                     SGHFPRRNRIISGLSLGVIVTEAAIDSGSLITARLAVEQGREVFAVPGFVRAETSRGT
                     HALIKEGAALIEEGQDVIDAIAPQLEPATRARLSAPRKKAVGEDFGNHERLVYDALSY
                     DPLTVDHLYEQTGLPVAAVMASLLALELQRRVRQLPGQRYLKA"
     gene            complement(4413..>5459)
                     /locus_tag="VFR82_05515"
     CDS             complement(4413..>5459)
                     /locus_tag="VFR82_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEU4501090.1"
                     /translation="SRRIARDEERARLKDIMKRVRNPGCGYIVRTVSEGVKEEELRSD
                     VDFLHVLWQDIKTKRDQLAAPALLHTDLSLSFRVVRDLFGKKVERLWIDSRQEYDAVR
                     DFVQRFSPEQTSRIHFYDKDESLFDHLGVEQEMARALSRKVWLKSGGYLVIDHTEAMT
                     VIDVNTGRFVGKRDQEETILRNNLEAAKEVAYQVKLRGIGGIIIVDFIDMEREKNRDK
                     VYHALVDAMASDKARTRISRISDLGLIEISRERVREDLLRSLSEPCRYCEGRGYTKSP
                     TTVAYEIFREIRKIGSGAEQQRIVVGAHPSVAGLLQDEERQGLEALERECAAKVHVMP
                     DEQLHLEQYDLVVM"
BASE COUNT         1064 a         1650 c         1456 g         1289 t
ORIGIN      
        1 gattggagcg ggcactgtgc ctcaactccc aacggcgggg acgactcttc ccgatgagcc
       61 ccccgcccgc ttcccctacg attctttctt gatctgctcg gcgagatagt tttccagccc
      121 gatctgcttg atcgtttcca tctgtgtttc gatccagtca atgtgctcat cgacatcttt
      181 ggccatgtct tccagcatgt ggcgcgtggt gaagtccatg actttcgcgc agtggacgac
      241 gccatcgttc agcagtgcca acatctcctg ttcggccttc agatcgagct tgagctgctc
      301 gggcacggtc tctccgacgt gaacggtatt cattcgctgc acgttcggaa ggccttcgag
      361 ataaagaatg tgtccgatca attcgtccgc gtctttcatt tcgtcgatgc tgcgctcgcg
      421 caccttgtga tagaggcgct cgtagcccca gttttcgcac atcttgccat ggacgaaata
      481 ctggttgatg gccgtgagat cggccgtgag aatcttattg aggagattca ccactccttc
      541 ctttgccttc atagctgtcc tcccttcacc aacgagtcag atgccgtttg tgcacaggca
      601 cgtgattcat tatcatcagg actgacagcg ctcgtcaact cacctgccaa cgcattttga
      661 tttcatctct cagtgtcgac gacatgtatt ctcaatctcg aacaatcaaa gaaaataatg
      721 gaacgaaaat tcgttgcttg atcaacgggg tcaagaggaa tgagaaggaa aggaaagcgt
      781 gcagagaagc gcttatcccg cttcgcggta gccgcagtct tcgttccgac actgcacgct
      841 tcggccggct tgcttgctca ctttttcgat gagaaacggc gcgctacagg ttgggcaggc
      901 cttcgggatc ggacgatccc acaaggcata ttcgcaggcc ggatacttgc tgcacgcaaa
      961 aaacgaccga ccttttcgcg tgcgcttctg gaccagatca ccaccgcagc caggctgtgg
     1021 acatttcacg cccaacgcga gcggcttggt cgttttgcat tgggggtacg cggaacaggc
     1081 aatgaacttg ccgaagcgac ctgtcttcac caacatcggg ctgccgcatt tctcgcagac
     1141 ctggtctgtg gtgagttcct gcttcgggac gattttgatc gtgccgtcag gcagtttctc
     1201 gaagttctgg gtgttcttac agggcggatc ctccttgtag ccgggacagg ccaggaactt
     1261 gccgttccgc ccccacttga tttccatcat ccggccgcat ttctcgcagg gaatgttcgt
     1321 tggaggctcg accgtatcct tcggcccggg aatgttcttg gccttctcca agtccgccgt
     1381 gaacgggtcg taaaaatcgc gaacggcatc gacccagggt ttgttgcctt cttcaacctc
     1441 atcgagttcc gcttccaact gtgaagtgaa gtcgacattc acgacatcgg gaaatccttt
     1501 caggaggaag tcgttgacgg tacggccggt ttcagtggga agaaaccgcc catcaacttt
     1561 ttcgacgtac ttgcgttcct gaatggtcga aatgatggcg gcatacgtcg agggccgtcc
     1621 aatgcctttc tcttccaatt ccttgatcag cagcgcctcg ttgtagcgcg ggggcggctg
     1681 agtgaagtgc tgcttcgaag tcaccccggg aatggtctgc tcggcctgtt cgaccaaacg
     1741 cagacgttcg ccctccgtca actcgggtag ttggcgctcg gctgtatcct ctgcctcttc
     1801 ctcctgcttg ggcttctgtg cgaacaattc tttgtcgact ccctccatat agacaatcgt
     1861 atgaccggga aacttcacga tcgtcccgct ggaacggaag acgtaccggg cggatgtctc
     1921 gagaggggtg gattcgaccc gtgtgacgtc taggatggcg gggaccatct gagaagcaat
     1981 gaagcggttc caaatcaact tgtacagatt gtattgatcc tgttccagat agggccggat
     2041 cgattcagga tcacgcgcgg ccgaggtcgg cctgatcgct tcatgagctt cctgtgccgc
     2101 cttcgaagtc ttatagacat tcggcacatc gggaaggtat tcggcgccga accgctcctg
     2161 aatcacctgc cgggcgtcag ccatcgcttc ctgtgagatt ctcggcgagt ccgttctcat
     2221 ataggtgatg agaccggtgg atccttcgct acctatctcc actccttcgt atagttgctg
     2281 cgcaagagtc atcgtctttt taggcgtgaa gtgcagtttc cgggccgcct cctgttgcaa
     2341 ccggctcgta atgaagggag cgacgggatt tcgtttcttt tccttccgct cgatggacgt
     2401 gaccgcaaac gctttgccct caatcgccga cacgatctgg ccagcctgtt cggcgtttcc
     2461 aatcgacgct tcttctccat tgatactatg gagcttggct tcgaacggcg gcgggttggt
     2521 tcccgcaagc agcacgatga tcgaccaata ctcctccgca cgaaacgctt cgcgctcggc
     2581 ctcccgctcg cacatgagtc gaacggccac ggattggacg cggcccattg agagacctcg
     2641 gcggaccttg ttccacagca attggctgcc ttggtaaccc acaatccgat caagaacacg
     2701 acgagcctgc tgtgcgttta ccagcttcat atcgatctgg ccgggagatt gcagcgcctg
     2761 cttgattgcc cgctctgtta tctcgttgaa gagaacccga aagactctcc catcgttctt
     2821 tttcttggac ttgccgcgca gctcctcaga gaggtgccag gcgatggctt ctccttctcg
     2881 atccgggtca ggtgccagaa agattttgtc tgcttcctca gccttcttct tgatatcagc
     2941 caacaccttc gactttccct tgatcgtgac gtactgcggc ttgaaattgt gctcgagatc
     3001 gacaccgagc ttgctggtgg gcagatcctt gatatgcccg accgacgcca tgacggtgta
     3061 gccccgaccc aaatatttcg taatcgtttt tgccttcgtg ggagattcta cgatgatcag
     3121 cgatttcgcc atgtcgactc cagattcaca actgtccctc tatcatccgt accaaatata
     3181 gcagatgagt gcaaccagac cgccggacaa tggacggttt acgccttgag ataccgctgt
     3241 ccaggtagtt gacgcacgcg ccgttggagc tcaagtgcca ggagcgatgc catcacggcg
     3301 gccacgggca acccggtttg ctcatagaga tggtccaccg tcagcggatc gtacgagagg
     3361 gcatcataca ctagccgttc gtgatttcca aaatcttcgc cgactgcctt ctttcgaggc
     3421 gcggagagtc gagcgcgcgt cgccggttcg agttgcggcg cgatggcgtc gatcacatct
     3481 tgcccctcct cgatcaatgc ggcgccctcc ttgatcaagg catgtgtgcc tcggctggtt
     3541 tcggctctca cgaatcctgg aacggcaaac acttcgcgtc cctgttcgac cgcgaggcgc
     3601 gcggtaatca acgatccgct gtcgatggct gcttccgtca caataacgcc aagcgatagc
     3661 ccgctgatga ttcgattccg tctcgggaaa tgaccgctat gcggcggagc atcgagcggt
     3721 aattccgaaa cgacggctcc tcgctcttcg atctcatgac ggagccgttc gtgttctgcg
     3781 ggatacgttc tcccgagtcc gcaacccagc acagcgaccg tgcggccgcc ggctgtaagg
     3841 gcacctcgat gcgcagcggc atcaactccg cgcgccaagc cgctgacgat ggtgaaccct
     3901 gaggctgcca actcgtttgc caaatgctcg gggatcaccc gtccagccgg cgtcgcacgc
     3961 ctcgcgccaa cgactgcaac ggccagttgg tcagattcct cgagcgagcc cgagacatac
     4021 aggagcggag gagggtctgc aatcgtccgc aggcgtgacg gataggcaga atccagaatc
     4081 gtccgcacgc tgatgcgctt ccgttcgatg gcactaagtt cccgtgcgat gcgccggcac
     4141 acttcacggt ccggtcctcg cctgattaca tcggccaatc gtgggctgca ccctctctgc
     4201 accagctcat ccgccggtgc tgcgagaacc gcatccggac tgtgccactc ccgcacgaga
     4261 gcgagaagcg tatgatcgcc tattccttcg agcgtgcgaa gcctcatcca tgcttcgagc
     4321 gaaccggttt ttgaagccgc gagagattgc acgtcgcctc acgtgtggcg ggactcgacc
     4381 gccggacacg tgcccggccg tggagttcgc tgctacatga ccacgagatc gtactgctct
     4441 aaatgcaatt gttcatcggg catgacgtgg actttggcag cacattcccg ctcgagcgct
     4501 tcaagtccct gccgttcctc gtcctgtagc agacctgcca cggagggatg cgcgccaacg
     4561 acaatccgct gttgctcggc gccactgcca atctttcgaa tctcgcggaa aatttcgtac
     4621 gccaccgtcg tcggtgactt cgtgtatccg cgaccttcgc agtaccggca cggttctgac
     4681 agcgagcgca gcagatcctc ccgcacacgt tcgcgggaga tttcaattaa tcccaggtcg
     4741 gaaatacggg aaattcgagt gcgagctttg tcggatgcca ttgcatccac cagggcatga
     4801 tacaccttgt cccggttctt ttcccgctcc atatcaataa agtcgacgat gatgatcccg
     4861 ccgattcccc gcaacttcac ctgataggcc acttccttag ccgcttccaa attgttacgg
     4921 agaatcgttt cctcttggtc gcgtttgccg acgaagcggc ccgtgttcac gtcgatcacc
     4981 gtcatggcct ccgtgtgatc gatcaccaag tatccgcctg acttcagcca cacctttcga
     5041 cttagcgccc ttgccatttc ctgctcgaca cccaggtgat cgaagaggct ctcgtcctta
     5101 tcgtaaaaat ggatacgcga ggtctgctcg ggagaaaacc gctggacgaa atcgcggacg
     5161 gcgtcgtact cctggcgtga atcgatccac aagcgctcga cctttttgcc gaataaatca
     5221 cggaccaccc ggaagctcag gctcaaatcc gtatgcaaaa gcgccggcgc cgccagttga
     5281 tcgcgctttg tcttgatgtc ttgccagagc acgtgcagaa aatcgacgtc ggatcgcagt
     5341 tcttcctcct tgaccccctc gctgaccgtt cggacgatgt acccacagcc gggattgcgg
     5401 acccgcttca tgatgtcctt caagcgcgca cgctcctcgt cccgagcgat tctgcggga
//
LOCUS       DASTQY010000172         9629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_803894, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000172 DASTQY010000000
VERSION     DASTQY010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9629
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803894"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFR82_05520"
     CDS             <1..541
                     /locus_tag="VFR82_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU4501091.1"
                     /translation="RASESQLESLNPMSLQQLSERVREHFAAGFVKAVEWRGDLAITV
                     TRDKLHEVAQFLRDDPAMDFDYIVHVSSVDWPDDEERFEVVYEFYSIRKRHRIRLKTR
                     VPESDCVVDSLTDLWKGADFMEREVYDMMGIRFRRHPDLRRILMPDDYTEGYPLRKDF
                     PLRGKGWRDTFEFLDETAR"
     gene            552..1796
                     /gene="nuoD"
                     /locus_tag="VFR82_05525"
     CDS             552..1796
                     /gene="nuoD"
                     /locus_tag="VFR82_05525"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEU4501092.1"
                     /translation="MAFEDQRTTVYKVDPAHPESETLPTLRTEELLLNMGPQHPSTHG
                     VLKVILELEGERLVKSTPVMGFLHRGVEKLAEEGTYHQFIPHTDRLDYVCAMYNNFAY
                     CRAVEKLMNITVPDRAEYLRTIVAEVQRIIGHQFWLGTQALDIGAMTVFFYCFRDREI
                     LLDWFDELCGARLTTSWYRIGGVERDFTPSLFAKLKQFLDYFPSKIDEYVIFLEKNRI
                     WIARTKGVAVISAEDAVNFGLSGPTLRGSGVDYDLRKAEPYSAYPKCEFSVPLGKNGD
                     TYDRYWIRVMELYESVKIVKQCLEQIQDGPIMADVPSVTLPPKDRVFTNLESMIQQFK
                     LFSQGFDAPPGEIYCGTEAHKGELGFYIVSTGGGKPYRLKIRAPSFIHMGAFDHMSRG
                     YMIADAITLFGTYDIVMGECDR"
     gene            1884..4166
                     /locus_tag="VFR82_05530"
     CDS             1884..4166
                     /locus_tag="VFR82_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU4501093.1"
                     /translation="MGLKPATNPDVEAATIELTIDGKTVTAKDGVSLYDVISSTGKII
                     PAMCYHYTFDPFGSCGMCLVMQEGKKAPVRSCTAKAAAGMIIRTEGDDLFQARKKAVE
                     KHLSVHPLDCPVCDADGHCELQDMAFQHGVTNLANAKQKFIPEDTRSPVLDFNMNRCI
                     ACAECINVCKDVLMIDALQFMKKGGFNQVVPKGDLPLACEFCGDCLAVCPVGAITNKF
                     SKYLYKPWQMKKTTTTCNYCGDGCQMYLETKDTEVIRVTSPLSWKNKWGDRADTAKGH
                     GGLCVRGRFGFEYIDSAARLKRPLLRKGNQLVEVPWLEAMHTIVDRFADIRKKHGPEA
                     IAGLITARCTNEELYLFQKLMRVAFRTNNLDSSARYGHMNFVRASKQALGVGRTPNDW
                     EDLTKAKAVVVIGSNLTETNPLTSVRIKEAIRVYKAQVVVFDSAVTNLGKLASHPVLI
                     KPGSEGMVIDGLVKAAVELDLVDEESVKKYPRAYAALKSAVAPLSLEQLAAKTGVPVA
                     RFKEAATIFAESPRSIILCAEGIVRQPDGYQNVLKLIDLAWITGKLGRPGCGVNTVTE
                     EPNEQGAADMGVAPEFLPGLASFDDQAIRDKLAKAWDATLPAIGTGAHLMEILKRCRS
                     GQIRALYVVGENPLGTLPASAEVRAALDRLELLVVQDPFLTDTGNMAHAVLPATTSAE
                     KDGTFTNLEGRVLRVRQAMDAIGESVPDWQIMTALANGLGCQWEYDSANDVQAEIMKL
                     LPGYYNLGQPKKIAPTADTY"
     gene            4245..4571
                     /locus_tag="VFR82_05535"
     CDS             4245..4571
                     /locus_tag="VFR82_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEU4501094.1"
                     /translation="MGQLLYHSGKLSTVAPGLIKIGPNTGRIRMNPADMERLGVQDGA
                     SVKLTSDRGSLQMGVQIDQSVAPHTCFFPEHFNEPPVKDLMSVHVDPVTGVPSFKRTG
                     VTIEKV"
     gene            4579..5166
                     /gene="nuoI"
                     /locus_tag="VFR82_05540"
     CDS             4579..5166
                     /gene="nuoI"
                     /locus_tag="VFR82_05540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEU4501095.1"
                     /translation="MTVRNATKKFLQAALFYEIWDAMKVTFKHMFHKPITFQYPREQR
                     AIPDAHRGALGLLRYDDGQERCVGCDLCEAACPSRCIKVISAEDVERPLQRYASEFYI
                     DITKCVFCGYCVEACPVNALAMTKMYEFSTHDKRTLLFDKKRLYDIGERHLDDAKKYL
                     YAHNQEKNIEESREYRYYFPQSVIKSTQPAPKHLS"
     gene            5180..5707
                     /locus_tag="VFR82_05545"
     CDS             5180..5707
                     /locus_tag="VFR82_05545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU4501096.1"
                     /translation="MIPVFFAYFALMSMAAAVLTVALKNPVHCGLALLALLLHVSGLF
                     VLLNAEFLWAVQVVVYAGAILVLYLFVLMLLNLKTDERYFHSTYLYFVVPTLLGSLYV
                     LALLFKSPYGGAKGDAPASAVLQEGDTYAIGMKMFSEYLLQFEIVGIFLLGAIIGAIV
                     LAKTPKPLEDMRGKN"
     gene            5765..6067
                     /gene="nuoK"
                     /locus_tag="VFR82_05550"
     CDS             5765..6067
                     /gene="nuoK"
                     /locus_tag="VFR82_05550"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEU4501097.1"
                     /translation="MVPLSAYVAVSAVLFITGLLGVLIRRNFIIVLMAVEIMLNAANI
                     NLVAFSHYLESMAGQLVALFVIAIAAGEAAVGLAIIIVVFRGKISTNVDEMNLLKW"
     gene            6369..8336
                     /gene="nuoL"
                     /locus_tag="VFR82_05555"
     CDS             6369..8336
                     /gene="nuoL"
                     /locus_tag="VFR82_05555"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU4501098.1"
                     /translation="MTDLLIKLIPVFPLLAVIANGVFGRRFWSHDWAHRLAWGSVGLS
                     FLCTILVFADIVRSGTPHEVVAYQWIFGGDLTINMAFLVDPLTCVMLLVVTGVGFLIH
                     VYSVGYMHGEDGFTRFFTYMNLFMVSMLLLVMGNNYAVLFIGWEGVGLCSYLLIGYYY
                     DKVSAAKAASKAFVVNRIGDAGFLLAIFLVFINFKTLDYTKVFAQVGHLSPEMATAIS
                     LCLLVGAVGKSAQIPLYTWLPDAMEGPTPVSALIHAATMVTAGVYMIVRNHVIFDLSP
                     TALGVVGLVGGATALFAATIGLVQNDIKRVLAYSTVSQLGYMFLGCGIGAYTAAIFHL
                     MTHAFFKALLFLSAGSVIHALSGEQDIRKMGGLSQKIPWTHKLFFIGTIAIAGIPPFA
                     GFWSKDEIMAHAFTHHHYVLYGMAAIGALLTSFYMFRLTYLTFYGQSRMDHHTQEHVH
                     ESPTVMIGPLMVLALLSLAGGFLGFPPDHGWLHQFLAPAAGASGEHEVSTGVVLSLMM
                     IASGIAVFGWGLAHYLYSMNPGAPARWAAKAGALYTTLLNKYYVDEFYDLVFVEPTKK
                     FGLLLDWFDRVVVDGVVRAVARLAELGAEGSTWVEKHVIYGGINVVGYGNHLMAKQWR
                     QLQSGMVHHYAAIIVAGLFLLAVVIQLIVQR"
     gene            8349..>9629
                     /locus_tag="VFR82_05560"
     CDS             8349..>9629
                     /locus_tag="VFR82_05560"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU4501099.1"
                     /translation="MLEQLDSTFPILTCLLFFPLVGAAVLWLFDDEDLIRTSALTITL
                     VELALAVFVLMRFVPDSPAMQFVEHVRWIPALGISYHVAVDGISVLFVGLTAFLTVLV
                     VIYSWDTIRYQTKLYMMALLALETTTMGVFVSLDLILFFVFWELMLIPSYFLIKLWGG
                     GAERHYAALKYVLYTLLGSVFMLVGIALLDINYHNYATLHHMDQLYSFDLLELLAIPV
                     PLNQQLLIFWLMFLGFAFKAPVFPFHTWLPDALLEGPIGMAVVLAGLKLGTFGFMRFS
                     IPLLPDASKHPTVVSVMMALGLAAIVYGAIVALMQPDFRRLLAYSSISHLGFIVVGLF
                     ALNYQGLQGSLLTMINLGFTTAGLFFIAGFLYTRQQTTQLQAFGGMAKHTPLLATFFL
                     LIGLASIGLPGTNGFVGEFLILLGTFQAHWLYGGI"
BASE COUNT         1969 a         2751 c         2728 g         2181 t
ORIGIN      
        1 cagagcaagt gaaagccagc ttgaaagtct aaatcccatg tcgcttcaac agttgtctga
       61 gcgtgtgcgg gaacactttg ccgccggctt tgtcaaagcc gtcgaatggc gtggcgatct
      121 ggccatcacc gtcaccaggg acaagctgca cgaggtcgcc cagttcctgc gcgatgaccc
      181 ggcgatggac tttgactaca tcgtccacgt cagttctgtc gactggccgg acgacgaaga
      241 gcgcttcgaa gtcgtctatg aattttattc aatcagaaaa cggcaccgta tccgcctcaa
      301 aacccgcgtg cccgagtctg actgcgtagt ggattcgctc acggatctgt ggaagggcgc
      361 ggacttcatg gagcgcgaag tctacgatat gatggggatt cgctttcggc gccatcctga
      421 ccttcgccgc atcctgatgc cggacgacta caccgaaggc tatcccttgc ggaaagactt
      481 tccactgagg ggaaagggct ggcgcgacac gttcgaattc ctggacgaaa ctgcaaggta
      541 gcacgttgcc gatggcgttc gaagaccaaa gaaccaccgt ttataaagtc gatcccgctc
      601 atccggagag cgagacgctt ccgaccttac ggactgagga gctgctgctt aatatgggtc
      661 cgcagcatcc gagcacccat ggggtcttga aggtgatcct cgaattggaa ggtgagcgtt
      721 tggtcaaatc cacgcccgtg atgggcttct tgcatcgcgg cgtcgaaaaa ctggctgaag
      781 agggcacgta tcaccagttc attcctcaca cggatcgact ggactatgtc tgtgccatgt
      841 acaacaactt cgcctactgc cgggcggtcg aaaagcttat gaacatcacg gtcccggacc
      901 gtgccgagta tcttcgaacg atcgtggccg aagttcaacg gatcattggc catcagttct
      961 ggttgggcac tcaagcgctc gacatcgggg ccatgacggt cttcttctat tgttttcgtg
     1021 atcgcgagat cctgctggac tggttcgacg aactctgcgg cgcacgcctg accaccagct
     1081 ggtaccgcat cggcggagtc gagcgggact tcactccctc gctctttgcc aagctcaaac
     1141 aattcttgga ctattttccc tcaaagattg acgagtacgt gatctttctg gagaagaacc
     1201 gcatctggat agcccgtacg aagggcgtcg cggtgatctc cgcggaggac gccgtcaatt
     1261 tcggcttaag cggcccaacg cttcgcggtt cgggggtgga ttatgacttg cgcaaggcgg
     1321 aaccttactc cgcctaccca aaatgcgagt tcagcgtccc acttggaaag aacggcgaca
     1381 cctacgatcg atactggatc agggtcatgg agctgtacga aagcgtgaag atcgtcaagc
     1441 agtgtcttga acagattcag gacggcccaa ttatggcgga tgttcccagc gtcacccttc
     1501 cgccgaaaga ccgcgtgttt acgaacctgg aatcgatgat ccagcagttc aagctctttt
     1561 cgcaaggctt cgatgctccg ccgggagaga tctactgtgg aacggaagcg cataaagggg
     1621 aattgggctt ttacatcgtc agcacgggtg gtggaaaacc gtatcggctc aagatccgag
     1681 ctccttcctt cattcatatg ggcgcgtttg accacatgtc gagaggctat atgattgccg
     1741 acgcgattac cctgttcgga acctatgaca tcgtcatggg agaatgcgac cgctagaccg
     1801 gtgacggtgg aatgccgaat ggagagcagg acggctcatt cagactgctc gggtgtttcg
     1861 cgttgatcat tgattgaggc accatgggat tgaaaccagc aacaaatccg gacgtcgaag
     1921 ccgcgacgat tgagttgacc attgacggca agaccgtgac ggcgaaagac ggcgtctcgc
     1981 tgtacgacgt gatttcaagc acgggcaaga tcattccggc catgtgctac cactacacgt
     2041 tcgatccctt cggctcctgc ggcatgtgtc tcgtgatgca ggaggggaag aaggcgccgg
     2101 tccgatcttg cacggccaag gccgcggccg gaatgatcat ccggacggaa ggcgatgatc
     2161 tgttccaggc gagaaagaag gcggtggaga aacatttgtc cgtccatccc ttggactgcc
     2221 cggtctgcga cgccgacggg cattgcgagc tgcaggacat ggccttccag cacggcgtga
     2281 cgaatcttgc caatgccaag cagaagttca tccccgagga tacgcgaagc cccgtactgg
     2341 acttcaacat gaaccgctgt attgcgtgcg ccgaatgcat caacgtctgc aaggatgtgc
     2401 tgatgatcga cgcactgcaa ttcatgaaaa agggcggctt caatcaagtt gtgcccaaag
     2461 gcgacctccc gctggcttgc gagttttgcg gagactgcct tgcggtctgt ccggtggggg
     2521 ccatcaccaa taagttctcc aagtatctct acaagccctg gcaaatgaag aaaacgacca
     2581 ccacctgcaa ctattgcggg gacggctgcc agatgtacct ggagaccaaa gatacggaag
     2641 tgatcagggt cacgtcgccg ttgtcctgga agaacaagtg gggcgatcgg gccgataccg
     2701 ccaagggtca tggtggtctc tgcgtgaggg gccgctttgg atttgagtac atcgattcag
     2761 cggctcgact aaaacggccg ctcttacgca agggcaacca gcttgtggag gttccgtggc
     2821 tggaagcgat gcataccatc gtcgaccggt tcgccgacat ccggaagaaa cacggcccgg
     2881 aggccattgc cgggttgatt acggctcgct gtaccaatga ggaactctat ttgttccaaa
     2941 agctcatgcg ggtggcattc cggacgaaca atctggacag cagtgctcgt tacggtcaca
     3001 tgaatttcgt ccgggcctcc aagcaggccc tgggtgtcgg ccgcacgccc aacgactggg
     3061 aagacctgac taaagccaaa gccgtggtgg tgatcggctc caatctcact gagaccaatc
     3121 cactgacttc cgttcgcatc aaggaggcca tccgcgtcta caaggcgcag gtggtggtct
     3181 tcgattcggc cgtcaccaac ctcggcaaac tggcgtcgca tccggtgctc atcaagccgg
     3241 gcagcgaggg gatggtcatc gacggcctcg tcaaggcggc agtggagctt gatctcgttg
     3301 atgaagagag cgtgaaaaaa tatccgcggg cctatgcggc gctcaaaagc gcggtggccc
     3361 ctctttcgct cgagcagctc gcggcgaaaa cgggagttcc tgtcgcacga ttcaaagagg
     3421 ctgcgactat ctttgctgaa tcgcctcgat ccattattct ttgtgccgaa ggcattgtgc
     3481 gccaaccgga cggctatcaa aatgtgttga agcttattga tctggcatgg atcacgggca
     3541 agctcgggcg tcccggttgc ggcgtcaata ccgtgaccga agaaccgaac gagcaagggg
     3601 cagccgacat gggtgttgca ccggagtttc tccccggctt ggcctccttt gacgaccagg
     3661 ccatccggga caaattggca aaggcctggg atgcgacgct ccccgcaatc ggaaccggcg
     3721 cgcatttgat ggagatcctc aaacgctgcc gtagtggaca gatccgcgcg ctgtatgtgg
     3781 tgggagagaa cccgttgggc acgctcccgg cgtccgcgga agtgcgagcc gccctggacc
     3841 gcctggaact gctggtcgtc caagatccct ttctgactga caccggaaac atggcccatg
     3901 ccgtattgcc ggcaacgact tcggcagaaa aagacggcac attcacgaac ctcgaaggcc
     3961 gggtcttgcg cgtgcgtcag gcgatggatg ccattggaga aagcgtccct gactggcaga
     4021 ttatgaccgc gctggcaaat ggattagggt gccagtggga atatgactcg gcgaacgacg
     4081 tgcaagctga gatcatgaaa ctgcttcccg gttactacaa tttgggccag ccgaaaaaga
     4141 ttgccccgac ggccgacacg tattagtcaa acggctatgc ggccggagcg gctgcgcgat
     4201 ataagagagc gccttctgac gggcagcggc cctttacctt gcagatggga caactactgt
     4261 atcactccgg caagctgtcc acagtagcgc ccggccttat caaaatcgga cccaatacgg
     4321 gccgcatacg catgaaccct gcggatatgg aacgcctcgg cgtgcaagat ggagcatcgg
     4381 tcaagctcac ctccgatcga ggctccctcc agatgggtgt gcagatcgac cagagcgtag
     4441 cgcctcacac ctgtttcttc cccgaacact tcaacgaacc gcccgtcaaa gacctcatgt
     4501 cggtccacgt ggacccggtg accggcgtgc cgtcattcaa gcgaaccggt gtgaccatcg
     4561 aaaaggtatg aagcgcccat gacggtcaga aatgcgacga agaagtttct tcaggctgcc
     4621 ctcttctacg agatctggga cgccatgaag gtaacgttca agcatatgtt ccacaagcca
     4681 atcacgtttc agtacccgcg agagcagcgt gctattccgg acgcgcaccg cggcgcgctc
     4741 ggattattac gttatgacga cgggcaggaa cgttgtgtcg gctgtgacct gtgcgaagcc
     4801 gcctgcccat cccgctgcat caaggtcatc agtgccgagg atgtcgaacg gccgcttcag
     4861 cgctatgcca gtgagtttta catcgacatc accaaatgcg tcttttgcgg gtactgcgtg
     4921 gaagcctgcc cagtgaacgc gctggccatg accaagatgt acgagttttc cacacatgat
     4981 aagcggacgt tgctctttga caagaagcgg ttgtatgaca tcggcgagcg gcacctcgat
     5041 gatgccaaaa aatacctgta tgcccacaat caggaaaaga acatcgagga aagtcgggaa
     5101 taccgttact acttcccgca gtcggtcatt aagtcgacgc aaccggcgcc gaaacatttg
     5161 agttgagcgg acgcggatca tgattcccgt tttcttcgcg tattttgcct tgatgagcat
     5221 ggccgccgcc gtgctcacgg tcgccttaaa gaatccggtg cactgcggac tggctttgct
     5281 ggcgctcctc ctgcacgtat ccggcctatt cgtcctcctc aatgccgaat tcctctgggc
     5341 cgtccaagtc gtcgtatatg cgggagcgat cctcgtcctc tacctgttcg tgctgatgct
     5401 gctcaatctc aagaccgatg agcgttattt tcacagcacc tacctgtatt tcgtcgtgcc
     5461 gacactcctg ggttcattgt acgtgctggc attattgttc aagtctccgt atggcggagc
     5521 aaagggcgat gcgcctgcca gcgcggtact gcaagagggc gacacgtatg cgattggaat
     5581 gaagatgttc agtgagtatt tactgcagtt cgagattgtc ggcattttcc tgttgggcgc
     5641 catcatcggg gcaatcgtgc tggccaaaac acccaaacca ctggaagaca tgagaggcaa
     5701 gaattgacga gataggccgt gcatgcctca cgtctgattc ctcgcgcctc tgattgagaa
     5761 cactatggtg ccattaagcg cctatgtcgc ggtcagcgct gtcctcttca ttacgggatt
     5821 actcggcgtg ctcatcagac gaaacttcat catcgtcctg atggcggtgg agatcatgct
     5881 gaacgcggcc aacatcaacc ttgtggcatt ttcccactac ctggagtcca tggcgggcca
     5941 gttggtggcg ctgttcgtca ttgccattgc cgccggggag gctgcggtcg gactggcgat
     6001 catcattgtg gtcttccgag ggaaaatctc cacgaatgtc gacgaaatga atctcctcaa
     6061 gtggtgaaga agctcactat gctgctggtc atcaataaca cccgcccgcc ccagcgcgcc
     6121 gagacgcact gttcaccgaa ctatctcacc cgcctacctc agcgatgctt ttgcacccac
     6181 tcgccccgcc gattggttcg caaccgcttt cccaagacgc gctgttcacg ggtgagggcc
     6241 gcagggcaat cacaacagct tctgcttgag cgaccgagaa caacgcctgg gccgaggcgc
     6301 gtcccggcgc cccagggctg ggtgggtgag ataggcgcga ttgtcagcgc cttcaagacg
     6361 agaccttcat gaccgatctt ctgatcaagc tcattccagt gtttccactg ctcgccgtca
     6421 tcgccaacgg cgtgttcggt cgtcgtttct ggtcgcatga ctgggctcac cgtctcgcct
     6481 ggggttcagt cggcctgtca ttcctctgca ccatccttgt gttcgcagac atcgtgcgca
     6541 gcggaacgcc acatgaagtc gtggcctacc agtggatctt cgggggcgat ctcaccatta
     6601 acatggcctt tcttgtcgat ccgctgacct gcgtgatgct gcttgtggtg acgggcgtgg
     6661 gattcctcat ccacgtgtac tcggtcgggt acatgcacgg agaagatgga ttcacgcgct
     6721 tcttcacgta catgaacctg ttcatggttt cgatgctgct cttggtcatg ggcaacaact
     6781 acgccgtgct cttcatcgga tgggaaggcg tcggcctgtg ctcttatctg ctgatcggct
     6841 actactatga caaggtttca gcggccaagg ccgcttcgaa ggcgttcgtt gtcaaccgga
     6901 tcggtgatgc agggtttctg ctcgcgattt tcctggtctt catcaatttc aagacgctcg
     6961 attatacgaa agtcttcgcg caagtgggtc acttgtcccc ggaaatggcc acggccattt
     7021 cactctgtct cctcgtcgga gccgtcggca aatcggcgca gatcccgctc tacacatggt
     7081 tgcccgatgc catggagggt cccacgcccg tgagcgccct catccacgcc gcgacaatgg
     7141 tgacggcagg cgtatacatg atcgtgcgca accacgtcat cttcgatctt tccccgacag
     7201 ccctgggtgt tgtgggattg gtaggcggcg cgacggcgct gttcgccgcg acgatcggcc
     7261 tcgtgcaaaa cgacatcaag cgggttctgg cgtactcgac cgtcagccag ctgggatata
     7321 tgttcctcgg atgcggaatc ggcgcctaca ccgcggctat ttttcatctc atgacgcatg
     7381 cgtttttcaa ggcgttgttg ttcctctccg ccggctcggt gatccatgcg ttatccggcg
     7441 agcaagacat tcggaagatg ggcggactga gccagaaaat cccctggacc cataaattgt
     7501 tttttatcgg aaccattgcc atcgcgggca ttcccccttt cgccggcttt tggagcaaag
     7561 acgagatcat ggcccatgcc tttacgcatc atcactatgt gctctatggc atggctgcca
     7621 tcggtgcgct cctgacctcc ttctatatgt tccggctgac gtacttgacg ttttacggtc
     7681 agtctcgcat ggatcatcac actcaagagc acgtccacga atcgccgacg gtgatgatcg
     7741 gtcccttgat ggtgttggcc ctcctctcat tggctggcgg attcctcgga tttccccccg
     7801 atcatgggtg gttacatcaa ttcctggcgc cagccgcagg tgccagcggc gagcatgaag
     7861 tcagcaccgg ggtggtcctg tcactgatga tgatcgccag cggcattgct gtattcgggt
     7921 ggggcttagc ccattacttg tacagcatga atcctggcgc tcccgcccgg tgggcggcta
     7981 aggcgggagc gttgtacacc accttgttga ataaatatta cgtcgatgag ttctatgatc
     8041 tcgtattcgt ggagccgacc aagaagttcg gactgctgct cgactggttt gatcgggtcg
     8101 tagtggacgg cgtcgtccga gcagtcgccc gtctcgctga actgggcgcc gaaggctcga
     8161 cctgggtgga gaagcacgtg atctacggtg gcatcaacgt cgtcgggtac ggcaaccacc
     8221 tcatggccaa gcagtggcgg cagttgcaga gcgggatggt gcaccactat gcggccatca
     8281 tcgtggcagg gctgttttta ctcgcggtcg tcattcaact gatcgtccaa cgctagacga
     8341 acgtcatcat gctggaacaa ctggattcga cattcccgat tctcacctgc ctgctctttt
     8401 tccctctggt tggcgccgcg gttctttggc tcttcgacga cgaggatctc atccgcacct
     8461 cggctctgac gatcacgctg gttgaactgg cgttggcggt cttcgtgctc atgcggttcg
     8521 tgcccgactc tcccgccatg cagtttgtcg agcacgtccg ctggattccc gctctaggaa
     8581 ttagttacca cgtcgccgtg gacggcatta gcgtgctgtt cgtcgggctg acggccttcc
     8641 tcaccgtact cgtggtcatc tattcctggg acacgatccg ctatcaaacc aagctgtata
     8701 tgatggcgct cctggctctc gagacgacga cgatgggcgt attcgtctcg ctggatctga
     8761 ttcttttctt cgtgttctgg gaactcatgc tgatcccgag ctacttcctg atcaagctct
     8821 ggggaggcgg cgcagaacgg cattacgcgg cattgaaata tgtgctctac acgctgctcg
     8881 gcagcgtgtt catgctggtg ggcatcgcgc tcctggatat caattatcac aactacgcca
     8941 ccctgcatca catggaccag ttgtactcct tcgatctgtt ggaactgctg gcgattcccg
     9001 ttccgctgaa ccaacaactc ctgatttttt ggttgatgtt cctgggcttc gccttcaagg
     9061 cgccggtgtt tccgtttcac acctggctgc ccgacgcctt gctcgaagga cccatcggca
     9121 tggcggtggt tctggccggt ctcaaactcg gaaccttcgg attcatgcgc ttcagcatcc
     9181 cgctgttgcc tgacgcctcg aagcacccga ccgtcgtctc cgtgatgatg gccctcgggc
     9241 ttgcggccat tgtgtacggc gcgatcgtgg ccttgatgca gccggatttt cggcgactcc
     9301 tggcctacag cagcatcagc cacctcggat ttattgtcgt cggcttgttt gcgttgaact
     9361 accagggtct tcaaggcagt ttgttgacca tgatcaatct gggtttcacc accgccgggc
     9421 tatttttcat cgccggcttc ctctacacca ggcagcagac gacacagctc caggccttcg
     9481 gcggcatggc caaacatacg cctttactcg cgacattctt cctcctgatc ggattggcct
     9541 caatcggcct gccgggcacc aacgggttcg tcggcgaatt tctgattttg ctgggaacct
     9601 ttcaagctca ttggctgtac ggcggcatc
//
LOCUS       DASTQY010000173         8330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1053359, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000173 DASTQY010000000
VERSION     DASTQY010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053359"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..512)
                     /locus_tag="VFR82_05565"
     CDS             complement(33..512)
                     /locus_tag="VFR82_05565"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HEU4501100.1"
                     /translation="MQKRLYSLARLTLARMTFVLLWSLSDAGHAAPSDGRFSLVMDGA
                     AVKDNQTGLIWEQEPDREHDVWSRSNERCAAKEVGGQKGWRGPSVDELKTLIDPAQRD
                     PALPAGHPFSNIKSEIYWTSTPDPKDDIVAWQISFYSGEPVTDQKSGTRRMWCVLAK"
     gene            655..1254
                     /locus_tag="VFR82_05570"
     CDS             655..1254
                     /locus_tag="VFR82_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HEU4501101.1"
                     /translation="MQDEERRRRIDVLAIGLFGLAVGALTLGIAQVGAISESNKVGTL
                     VIALMFGGVVQILAGITDIRYHEQLGGTALTMYGFFWTTVCIAKLVSESTSFHLDMTL
                     YAPINFVYAAFSAVMVYLTAYRNVTLCMLHIVITVTFSSSVLVQLDLITETVPGLFHM
                     IVGFMAFYHAVASLTQAFTGRQLVPLGPPLLQQDVSMAR"
     gene            complement(1284..2249)
                     /locus_tag="VFR82_05575"
     CDS             complement(1284..2249)
                     /locus_tag="VFR82_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU4501102.1"
                     /translation="MDHPLGELKTSGPADAVRTPVEHESESEMLGAEEESPFSRWTLP
                     LLLFLLTVFTTLWAGAYQAYSGTIRGPLAFLWEHPEMLWHGIPFAGTLLFILVTHEFG
                     HFILSKIHRVPASLPLFIPGPPHFIGTFGAIIRMRGPILNRRALFDIGVAGPLAGFVV
                     AVVALIIGLFLSTVVDRTTTFGLQLGEPLLLKFMAWLIIGPLPPEADVLLHPVAFAAW
                     FGLFVTSLNLIPIGQLDGGHVAYALWGEGQRTMAFAVLPLLLMLGFVGWPGWFVWAFM
                     AGVWGLAHPPVLDPHAPLGRGRILVGWIAFAVFVLTFAPIPFSFH"
     gene            complement(2260..3102)
                     /locus_tag="VFR82_05580"
     CDS             complement(2260..3102)
                     /locus_tag="VFR82_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4501103.1"
                     /translation="MPLEDDLCDIIKKARTGLKLAVGDVARLTGLPGADITALERGDQ
                     LRDRTELRAISTALGLRFAALEQIALEKWVPKTLPLMPGIETVLGEISGYGVKGYIVH
                     DGGEAALVDTAYNADMMLEILEARRLRLVGICLTHGHADHAEGIHQIVSRWPVPIYLG
                     TEDVTLLHWKPSEQQRATPHDGRTIQVGRLTLTCLTTPGHTPGGVCYRLEMPWGPVAF
                     VGDTLFAGSIGRSNPPMLYATHLESVRRYVLTLPADCLLLPGHGPGTTVREELDHNPF
                     YVTR"
     gene            complement(3384..4838)
                     /locus_tag="VFR82_05585"
     CDS             complement(3384..4838)
                     /locus_tag="VFR82_05585"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU4501104.1"
                     /translation="MMTRHAFLTAGAKLAGVFVIGLLAAVMTFAADVTLDPRKMNFTP
                     VEFSPPEPARVVLENGMVVYLLEDHELPLVNVTATMRTGGWLEPQDKIGLAALTGSVM
                     RSGGGGGLTAEQIDEELEQYAIDIGISMSRQSGSASLDVLRKDLGRGLRIFAGLLRQP
                     AFEVGRVELAKLQMIEGIRRRQDSPGSIVGREFLKQLYGPDHPSARESTIDSVKRITR
                     DDLIAFHQRTVQPNGIILGITGDFQQAEMLALLRETFGNWKKGDVPDLNIADARVSAG
                     PSIVRFVNKDTSQTHLRIGHLSIKEHDPDYVALAIANDILGGSSFRSRLFNDVRTKRG
                     LAYSVGSRLNVGMHDQGVWLMRAETKLPSTQEVISRFVANMERMRTELVTNEELAEAK
                     EAYVNSFVFSFASPSAIVGRFVELEYDGLPKDFLQQLRARVVALTREDVLAAAKKHFH
                     PDRLTIVAVGPGEALPKLLSSFGEVKEIKLIPEG"
     gene            complement(4835..6448)
                     /locus_tag="VFR82_05590"
     CDS             complement(4835..6448)
                     /locus_tag="VFR82_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU4501105.1"
                     /translation="MKRTGTNRAGLAQPMRRVSRWIQACCVAVVLGLASLSAAGSSGL
                     ADRVIEHKLANGLTVLLVERHQAPVVSINMTFGVGGVNEQVGQTGLAHLYEHMAFKGT
                     RTVGTKNYEKEKPILDELHRVGTELEQRQRELARRTADLPVDPTETRTIESLQKRFKE
                     LQDQAAQYVAGNEMALLYQRHGGVGLNASTGKDLTRYVISLPANRLPLWAAIESDRMA
                     SPVLREFYKERGVVMEERRLRNDDSPSGLLYETFTSAAFRAHQYGVPTIGWESDILSL
                     TPAVTEAFFKTYYGPGRATIGIVGDIDAKEVMTLIENTFGKIPAAPPPPQIVTVEPPQ
                     RGERRVEVEFDAEPSLAIGFHKPGLGHPDDYVFDVIDELLTDGPTSRLYSKLVRDSRL
                     AASVNSDANYPGVRSANLFVLSATPLAPHSTAEVETAILAELERLKTEPVAPKELERI
                     VNNLDADMVRALRSNSGLASQLALYQTVAGDWRFILKSRDRIAAVTPADIQRVATQYF
                     TKSNRTVATLVKKTTSKQVATVTSDKGVR"
     gene            complement(6445..7092)
                     /locus_tag="VFR82_05595"
     CDS             complement(6445..7092)
                     /locus_tag="VFR82_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU4501106.1"
                     /translation="MGKAPIDLLIFDLDGTLIESKWDIAASVNFTLEELGLPQRPLEE
                     IFGFVGDGVKKLLRLAVGEANRVSFEEALGVFRRHYLAHCLDRTVYYPGVEKALGYFA
                     DKRKAVATNKSIEYTNVILQGLGPQYFDFVVGGDNGFGLKPEPGMLLHIMERLNAAKE
                     RTVLVGDSTNDINGGHNAGIRVCAVGYGMGNRERMSACQPDWFIERPEELMEIFE"
     gene            complement(7101..7676)
                     /locus_tag="VFR82_05600"
     CDS             complement(7101..7676)
                     /locus_tag="VFR82_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501107.1"
                     /translation="MLLRKPIAIAGRSIMGIMWMLSALAWFNGAALAQTGGLSHSPAE
                     IVKKYFELDQKGAKLDSMSFEALAPYRSWQAEPAWGKVVVIRGFSVPEHHRHWEIVNP
                     LEVVIPVTFQVIGSVYLETAGFLPDDTVEEIRVRVKSIRNRWRIIEPVLPPHVGQKRM
                     INYVREAWVKETEPAKRESLAVLQDELRKVK"
     gene            7871..8290
                     /locus_tag="VFR82_05605"
     CDS             7871..8290
                     /locus_tag="VFR82_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HEU4501108.1"
                     /translation="MSSPITYWVLLLSVLCVAAPCGSPSESRAHSVELANEAVVTPDR
                     PSLVPRSDIERTAPQKAYELLAALQQRHGAPLPGYIGGNKFRNRERRLPHGRYREYDV
                     NRKTPGRSRGAERLVIEQHSGKAYYTDDHYRTFIPLN"
BASE COUNT         1728 a         2535 c         2319 g         1748 t
ORIGIN      
        1 ctcaccgtga cagacaacgg cgtcacaacc gactactttg ccaggacaca ccacatccgg
       61 cgtgtgccag acttttggtc cgtgaccggc tcaccgctgt agaagctgat ctgccaggca
      121 acgatgtcat ctttgggatc aggcgtgctc gtccagtaga tttctgactt gatattcgaa
      181 aagggatggc cagccggaag ggcgggatca cgttgggccg gatcgatcag ggtcttgagc
      241 tcgtctacag aaggaccgcg ccagcctttc tgtccaccca cctccttggc cgcacagcgt
      301 tcgttggagc gactccacac gtcatgttcc cggtcgggtt cctgctccca gatcaacccc
      361 gtctgattgt ccttcaccgc ggcgccgtcc atcacaagag agaatcggcc atcgcttggt
      421 gcggcatgac ccgcatcaga aagggaccag agcaggacga acgtcatacg cgcgagcgtc
      481 agtctcgcaa gcgaatacag tcgcttttgc atgggtgatc tccgttcttt cggttatgga
      541 ataagagtgt gacgaatgca gcttgtcttt tcaatggtca gtcaggcatg ctcagcgcgc
      601 ttttgccgct ttagcttcgc acctgtttcc taccagacca tgaaggaggc gctcatgcag
      661 gacgaggaac gacggcggag gattgatgtc ctcgccatcg gattatttgg actggctgtc
      721 ggggccttga cactcggcat cgcacaggtt ggggcaattt ccgagtcgaa taaggtcggc
      781 accttggtca ttgcgctgat gttcggcggc gtggtccaga tattggccgg cattacagat
      841 attcggtatc acgaacaact cggcggcacc gctctcacca tgtatggatt tttctggacg
      901 accgtttgta tcgcgaagtt ggtcagcgaa agcacgtcgt tccacctcga tatgacgctc
      961 tatgctccca tcaatttcgt ctatgcggcc ttttcggccg tcatggtcta cctcacggcc
     1021 taccgcaatg tgacgctctg catgctccac attgtcatta cggtgacctt ttcctccagc
     1081 gttctcgtgc aactcgatct gatcactgaa acagtgcctg gccttttcca tatgattgtc
     1141 gggtttatgg ccttttacca tgccgtcgcc agcttgacgc aggctttcac ggggcggcag
     1201 ctggtcccgc tcggccctcc ccttttgcaa caggatgtga gcatggcacg ataggaatgc
     1261 ccgagcagcg cggccttaac gtgctagtgg aatgaaaagg gaatcggcgc aaaggtcagt
     1321 acaaacacgg cgaacgcgat ccagccgacc agaattcttc ctcgtccgag aggtgcgtgc
     1381 ggatcaagaa ccggtgggtg ggcgaggccc cacacaccgg ccatgaaggc ccacacaaac
     1441 catcccggcc aaccgacgaa tcccaacatc agcagcaacg gaaggacggc aaaggccatc
     1501 gtccgctggc cttcccccca taaggcatag gccacgtggc cgccgtcgag ctgcccgata
     1561 ggaatcaaat tcaacgacgt cacaaaaaga ccgaaccagg cggcaaacgc gaccggatgg
     1621 agaagcacgt cggcctccgg cggcaatggg ccgatgatca gccaggccat gaatttcaac
     1681 aacagcggtt cacccaactg caggccgaag gtcgtcgttc gatcgacgac ggtcgacagg
     1741 aataagccga tgatgagcgc gacaacggcc acaacaaacc cggccaacgg gccggcgacg
     1801 ccgatatcaa agagggcacg ccggttcaga atcgggcctc gcatgcgaat aatcgcgccg
     1861 aacgtcccga tgaagtgagg cggcccgggt atgaagagcg gtagcgaggc cggaactcga
     1921 tggattttgg acaagatgaa gtggccgaac tcgtgcgtca ccaggataaa cagtaacgtt
     1981 cccgcgaatg gaatgccatg ccagagcatc tccggatgtt cccagagaaa cgccagcggt
     2041 cctcgaatcg tgccgctgta ggcttgatag gcgcctgccc agagcgtcgt gaaaacggtc
     2101 aacagaaaca ggaggagggg taacgtccag cgagaaaagg gcgattcctc ttcggcgccc
     2161 aacatctcgg attcagattc atgttcgaca ggcgtccgga cggcgtccgc tggcccggaa
     2221 gtcttgagtt cgcccagtgg gtgatccatg ggatcccgcc taccgtgtca cataaaaagg
     2281 gttatgatcc agttcttctc gcactgtcgt gccagggcca tgtcccggca acagtaaaca
     2341 gtcggcgggc aacgttaaca cgtagcgacg aaccgactcc agatgggtcg catacagcat
     2401 gggcggattg gagcggccaa tggagccggc gaacagggta tcgcccacaa acgccaccgg
     2461 accccatggc atctcgaggc gatagcacac tccccccggc gtatgtcccg gcgtggtcag
     2521 gcacgtgaga gtcaaccgtc ctacctgaat ggtccggcca tcgtgcggcg tggctcgctg
     2581 ttgctctgaa ggcttccagt gaagaagcgt cacatcctct gttccgagat agatgggtac
     2641 gggccaacga ctcacaatct gatgtatccc ttccgcatga tccgcgtggc cgtgggtgag
     2701 acaaatgcct accaagcgca gacggcgggc ctcgaggatt tccagcatca tgtcggcatt
     2761 atatgccgtg tcgacaagcg ccgcctcgcc gccatcatgc acgatgtagc ctttcactcc
     2821 atagccgctg atttcgccca gcacggtttc gatgcccggc atcaagggca gcgtcttggg
     2881 aacccacttt tcgagagcga tctgctcaag cgctgcgaaa cgcaaaccga gggcggtcga
     2941 aatggcccga agctcagtgc gatcgcgaag ttgatcgcct cgttcgagcg ccgtgatatc
     3001 cgctcccggc aagccggtga gacgcgccac atccccgacg gccagcttca atcccgttct
     3061 cgccttctta ataatgtcac agagatcgtc ttccagcggc attgtaggcc ttagcagtta
     3121 gttatccacg agtcagcagt tgaattgctt gtcgctgatc tttctcgcct tatggagtcc
     3181 agcaacctct gcggctggtg ccctcctgat gtggcactga acgacgaatg aggtccggcc
     3241 gtcgatgccc ttggtctgag atggacggat ttgaaacgcc cacattccga tgtggagacg
     3301 aggggcgccg aagccgtttt ccagcagcta cagcagccag gcccagtccg catcgaagcg
     3361 aagccaatgt gaacggatgc ctctcaccct tcagggatta acttaatttc ctttacctcg
     3421 ccgaaactgg acagaagttt cggcaacgct tcgccggggc cgactgccac gatcgtcagg
     3481 cggtctggat ggaagtgttt tttagccgcg gcaagcacgt cttctctggt caacgccacc
     3541 actcgagcgc gtaattgctg cagaaaatcc ttgggcagac cgtcgtactc gagttcgaca
     3601 aagcgcccga cgatggccga tgggctcgcg aacgaaaaca caaatgaatt gacgtacgcc
     3661 tctttcgctt ccgccagttc ttcattggta actaactccg tacgcattcg ttccatgttg
     3721 gcgacgaacc ggctgatgac ctcctgggtg gagggcaact tcgtttcagc ccgcatcagc
     3781 cacacccctt ggtcatgcat gccgacgttc aaccggctgc cgactgaata cgcgaggccg
     3841 cgtttcgtac ggacgtcgtt gaagaggcgg ctacggaacg agctgcctcc gaggatgtcg
     3901 ttggcaatgg ccaaggcgac ataatcgggg tcgtgctctt taatgctcag atggccgatc
     3961 cgcaaatgcg tctgggacgt atccttattc acgaaacgga cgatcgaagg cccggcgctt
     4021 acccgcgcgt cggcgatgtt caaatccggc acatctccct tcttccagtt gccgaacgtc
     4081 tcgcgtagca acgcgagcat ctcggcctgc tggaaatcgc ctgtgatgcc gagaatgatg
     4141 ccgttcggct gaaccgtccg ttggtggaac gcgatcaaat catcgcgggt aattcgcttg
     4201 accgagtcga tcgtgctttc cctcgcgctg gggtggtccg gcccatagag ttgcttgaga
     4261 aattctcggc cgacgatcga accggggctg tcctggcggc ggcgaatccc ctcaatcatc
     4321 tgcagcttgg ccagttccac gcgtcccact tcgaacgcgg gctggcggag cagaccggcg
     4381 aatatccgga gcccgcgtcc caggtccttt cggaggacgt cgagtgaagc tgaaccggac
     4441 tgtcgactca tcgagatccc aatgtcgatg gcgtattgtt cgagctcctc gtcaatttgc
     4501 tcggccgtta aacccccgcc tcctccgctt cgcatgacgg atccggttaa ggcagctagt
     4561 ccaatcttgt cctgtggttc gagccaccca ccagtccgca tcgttgccgt aacgttcacg
     4621 agaggcaatt catggtcttc gagcaaatac actaccatgc cgttctcaag cacgacgcgc
     4681 gcaggctctg gcggcgaaaa ttccacgggc gtgaaattca tctttcttgg atcgagcgtg
     4741 acgtctgcag caaaggtcat caccgctgcc aacaatccaa tgacgaacac ccctgcgagc
     4801 ttcgcccctg ctgtgagaaa tgcgtgcctc gtcatcatcg taccccctta tccgacgtca
     4861 cggtcgccac ctgcttcgac gtggtctttt tcacgagcgt ggccacagtg cgattggact
     4921 tggtgaagta ctgggttgca acacgctgaa tgtcggcggg cgtgaccgcg gcgatcctgt
     4981 ctcgcgactt gagaataaag cgccagtctc cggcaacggt ctgatagagg gccaattggg
     5041 aagccagccc gctgttagag cgcagcgccc ggaccatgtc ggcatccaga ttgttcacga
     5101 ttcgctcgag ctccttaggg gcgaccggtt cggttttgag ccgctcaagc tcggccaaga
     5161 tggcggtctc gacctccgcg gtggaatgag gcgccagcgg cgttgcactg agcacaaaca
     5221 aattggccga gcgaacaccg ggatagttcg cgtccgaatt caccgaagcg gccaagcgag
     5281 aatcgcgcac cagcttgctg taaagccgcg atgtcggtcc gtccgtcaat aactcgtcta
     5341 tgacgtcgaa cacataatcg tcgggatggc ccaggcccgg cttatggaag ccgatggcca
     5401 acgacggctc ggcgtcgaac tcaacttcca cacgccgctc gcctcgttgc ggcggctcca
     5461 cagtgacgat ctgaggcggt ggcggtgcgg ccggaatctt accgaacgta ttctcgatta
     5521 aggtcatgac ctccttggcg tcgatatccc cgacgatgcc gatcgtcgcc cggccgggac
     5581 cataataggt cttgaaaaag gcttccgtga cggcgggggt caatgacaag atgtccgact
     5641 cccagccgat ggtcggcact ccgtactgat gggcccggaa ggcagccgac gtaaaggttt
     5701 catacagcaa gccgctgggg ctgtcgtcgt tccgcaaacg gcgctcttcc atcacgactc
     5761 cccgttcttt gtaaaactcc cggagaaccg gactcgccat gcgatccgat tcgatcgcgg
     5821 cccaaagggg tagccgattt gccggcagac tgattacata gcgggtgaga tcctttccgg
     5881 tcgatgcgtt gagaccgacg ccgccgtggc gttgatacag gagtgccatc tcattaccgg
     5941 ccacgtactg ggcggcctgg tcttgtagct ccttgaaccg cttttgcaga ctctcaatgg
     6001 tgcgcgtctc ggtggggtca accggcaggt cggcggtacg ccttgccagc tcccgctgcc
     6061 gctgttcgag ctcagttccc actcggtgca attcgtcaag gatcggtttt tctttctcgt
     6121 agttttttgt cccgactgtc ctcgtgcctt tgaatgccat atgttcgtat aagtgggcaa
     6181 ggccggtttg gccgacctgt tcgttgaccc cgcccacacc gaaggtcatg ttgatcgaca
     6241 cgaccggcgc ttgatgccgt tccaccagca gcacggtcag gccgttcgcc aatttgtgct
     6301 caatgactcg atccgccagg ccggaagaac cggcagcact caagctcgcc agaccgagca
     6361 cgacagccac acagcacgct tggatccagc gtgacactcg cctcatcggt tgcgcgaggc
     6421 ccgctctgtt cgtccccgtt cttttcattc gaatatctcc atcaattctt ccggtcgttc
     6481 aataaaccaa tccggctgac aggcggacat ccgttcacga ttacccattc cgtagcccac
     6541 cgcacacacg cgaataccgg cgttgtgccc gccgttgatg tcgtttgtgc tgtctccgac
     6601 cagcaccgtc cgctcttttg cggcgttcag ccgttccata atatgcaaca acatgcctgg
     6661 ctccggcttc aatccgaatc cattgtctcc gccgacgacg aaatcgaaat actgcggccc
     6721 cagtccttgc agaatcacgt tggtgtattc gatcgacttg ttcgtggcga ctgcctttcg
     6781 tttgtcggca aaatatccaa gcgctttttc cactcccggg tagtacaccg tccgatcgag
     6841 gcagtgggcg aggtagtgcc ggcgaaacac ccccagtgcc tcttcgaagc tcacgcgatt
     6901 ggcctcgccg acggccagcc ggagcaattt cttgacgccg tcgccgacaa acccgaaaat
     6961 ttcttcaagc ggccgttgcg gcagccccag ttcttcgagc gtgaagttaa ccgaggccgc
     7021 aatgtcccac ttcgactcga tgagcgtgcc gtccaggtcg aaaatcaaca agtcgatcgg
     7081 cgccttaccc attgccctcc ctacttgacc tttcgcaatt cgtcctggag tacggccagg
     7141 ctctcgcgtt tggccggttc cgtttccttc acccaggcct cccgcacgta attgatcatc
     7201 cgcttctggc cgacatgcgg gggcagcacg ggttcaataa tacgccagcg gtttcgtatc
     7261 gatttgactc ggacgcgaat ttcttccacc gtgtcgtccg ggagaaaccc cgcggtctcg
     7321 aggtagactg agccaatgac ttgaaatgtg actggtatca cgacctccag cggattcacg
     7381 atttcccaat gccgatgatg ctcagggacg gaaaatcccc ggatgacgac aacctttccc
     7441 caggctggct cagcctgcca gcttcgatag ggcgccaacg cctcgaacga catcgaatcg
     7501 agcttagcgc ccttttgatc gagttcaaaa tacttcttca ctatttcggc gggagaatgg
     7561 ctcaagcctc ccgtttgagc caatgcggcc ccgttgaacc atgcgagcgc actcagcatc
     7621 cacatgatcc ccattatgga tcgaccagcg atcgctatgg gcttgcgcag cagcatcgtc
     7681 ggcatgctcg ggcggtcgag atggttctca gttgtcgcgg gaagaattca caccggcgac
     7741 gacattctac caaaggcgtt cttcgaaagc atgtttcgaa gggacggcta agatgacggc
     7801 gttgacggat ctcaagcctg catgctagcg tttccgcagc cgcaaccctc ttccgcaagc
     7861 cgttgatgag atgtcgagtc ccatcacgta ctgggtgctt ctgctctctg tcctctgcgt
     7921 ggcggcgccg tgcggaagtc cttctgagag ccgcgcccac tcagtcgagt tggcaaacga
     7981 ggccgtggtc acgccggacc ggccctcgct tgttccgcga tcggacatcg agcgcacggc
     8041 accgcaaaaa gcctatgagt tactggcagc gctccaacaa cggcatggag cccccctccc
     8101 tggttacatc ggcggaaaca aatttaggaa tcgggaacgc cgactgccgc atggccgcta
     8161 tcgggaatat gacgtcaatc ggaaaacacc cggccgatcg cgcggcgcgg aacgcctcgt
     8221 catcgaacag cacagcggca aggcctacta tacggatgac cattaccgga cgttcattcc
     8281 attgaattag acgacatggc ttcgcacaca tcgccgctgt ctccgacacg
//
LOCUS       DASTQY010000174        11231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_940881, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000174 DASTQY010000000
VERSION     DASTQY010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11231
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940881"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFR82_05610"
     CDS             complement(<1..680)
                     /locus_tag="VFR82_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU4501109.1"
                     /translation="MMRRLREKFVALTMLIVFLFYVGYRIYETKSDAAALQVKTLEDA
                     VPTVAIVHPKPADASETIILPGNITAWFEAPIYAQVSGYVKMWYKDYGAQVKKGDILA
                     EINAPALDAQYAQAKADLESERAKYALADITAKRWLALRPNQAVSEQSISVKVAEAKA
                     EAAKVKASEQNVRNYEALIRFKTIVAPYDGVVTVRNINVGDYVNKDGTISTPGSISNL
                     FTVADVSM"
     gene            complement(680..2362)
                     /locus_tag="VFR82_05615"
     CDS             complement(680..2362)
                     /locus_tag="VFR82_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU4501110.1"
                     /translation="MSPQLDQRMQPSRNVFVRFQKGFERRFDRFREGYGTLLERVIAR
                     RSAFIKISLAFAVGSTVLFYFLGRDYFPEIRSGVIQMHMRAPLGTRIEVAGRLATLVS
                     NSVEELLPGQVENIVSNCGLPVGPHNLAFIPTPTIGSQDCDLTILLEDEKSPVWDYRR
                     ILRKGLRERYPGTEFTFQPSDLTAKILNFGAPAPIDVQVNGPDIYANYEFVRKLVGKF
                     REVPGAVDVVIQQTMRTPTLMVEGNRTFGLGVNRTLKDMADNLLMTTAGSQQVDQIYW
                     LDPTTGMSYLINVYTPQPQINSLNSLKTIPIDSSANPSKDNDIQLLGNLADLSAEGTP
                     GVITHGNIMPLFDIYISAEGRDLGGVLTDVQQIARSMESEKPRSAELEIHGQASLMHE
                     AYSELIFGLLAAIVLVYLLIVVNFQSWLDPFIIITALPGALAGIAWALFLTHTRLSEP
                     ALTGAILTMGTATANSILVVSYARERIQEHGDALRAAIEAGKTRFRPVIMTASAMIIG
                     MVPMATGYSQNAPLGRAVIGGLFVATVFTLLFVPCVYAMFYNRRMTRQKERA"
     gene            complement(2346..3845)
                     /locus_tag="VFR82_05620"
     CDS             complement(2346..3845)
                     /locus_tag="VFR82_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU4501111.1"
                     /translation="MNQIVLIALRRPYTFVVMSILILVLGTKTVLKMPTDVFPNITIP
                     VTSVVWIYTGLLPQQVEGRITYLFERFLTSTVEGIKYIHSHSYYGSSITNIYLQDGVD
                     VGRAEADIVGIAQNVVKALPPDISPPMVMRLAPSSIPVAMIQISSDNMTPAELYNLAY
                     MRIRPLLVTVPGIVLPHPYGGQDMQVMVNLDQQKLLARHLTPTDIHNVLMQQYLVLPS
                     GDIKIKSTDWIVLTNASPLKIEDFGDIPIKREGNAFIYLRDVATVQLMGRVQQNAVLV
                     KGKQTVIIVAMKSTEASTLDVVDGIKKMIPRAEQVSPPGVKIKLLDDASTFVKDSISD
                     VVYEMATAGALVGLIVLLLLGSWRATVIVWTSIPLSILTAIIGLHWLNETINVMTLGG
                     LALAVGILVDDATVMIENIDRHLEMGKPLEQAIIDAANQIVVPTLVATLCIAIVWIPL
                     FELGGVAGYLFKPMAEAVIIAMLASFILSRTLVPTMAKYLMKSHHVAAA"
     gene            4266..5039
                     /gene="modA"
                     /locus_tag="VFR82_05625"
     CDS             4266..5039
                     /gene="modA"
                     /locus_tag="VFR82_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4501112.1"
                     /translation="MAVILAGAMIGFGMGVPASGETLTVAAPPSLRAPFQEILRMFEQ
                     EYGVSVDVRYGPSHTFRRQIEQGAPLDVFLSDSWQEVETLHQKGLTLFEGPLRYAQTS
                     MVVVMSSASAATVISVSEASSHGALRVALVDPKGSSAGAIAVKALKQMDPVAHHHARL
                     IATPNTDEVVRLVSGGEADVGVVYRADAINSGLVRIIDEAPAGRSIPVVFGQAVVSTC
                     REASRKAGEQFLSFMMSPRIQKLLLKYGFDAVPTKATVG"
     gene            5472..5990
                     /locus_tag="VFR82_05630"
     CDS             5472..5990
                     /locus_tag="VFR82_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501113.1"
                     /translation="MAFLPFRDRTRIVLFRWPAIIAGLSVALGIHIVLTAFGLGVVFL
                     QSQSAEGGSSMATAALLLWSGLSWVMAAFIGGYITAWVADASRYVESLFHGLVLWGAL
                     TFILMFLPPSTMGISAEAESALSPATISSVAWFVAIGGLFSLGTTIWGAVIGSRVVAE
                     VETKQTGTYRAA"
     gene            6000..6317
                     /locus_tag="VFR82_05635"
     CDS             6000..6317
                     /locus_tag="VFR82_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501114.1"
                     /translation="MVQRRKKDNEQKAAEIAKWINPNERITIQFDDQRDLSAEVTGCT
                     DHLVSLSLDTAVPHIRQHVSLPLNFVEVSEDLSHYTRDPERPLTRKRMMLIVQGERPA
                     VIY"
     gene            complement(6307..6540)
                     /locus_tag="VFR82_05640"
     CDS             complement(6307..6540)
                     /locus_tag="VFR82_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501115.1"
                     /translation="MTEALEQLEGIAQVQADAIPNHVLIDHDGRHRTQEELVALLNQL
                     IIAHGGCRAAVMKSCITAGSSAFSAPSASPLSR"
     gene            complement(6689..7561)
                     /locus_tag="VFR82_05645"
     CDS             complement(6689..7561)
                     /locus_tag="VFR82_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU4501116.1"
                     /translation="MKAVETEVVIEGSGEKYAAWTFDGQVPGPVVRVTEGDTVKFTLT
                     NPATNKYPHAMDFHAAEIDFLNNYKAINAGETISYTFVAKKPGIFFYHCGAPPMIQHV
                     ARGMFGAIIVDPKNASAWPKADREYVLIQSEYFKNPDDVQAMFDRKFDGVMFNGGLFK
                     YHPFVSGGGKLDAKPGERVRIFFVNAGPNEFSAFHPIGEIWDNVYESGNPANKLTGVQ
                     TYVVGPGSAAIFDVVAESAGAYPLVTHSLTAALRGAIAVLVASPEAKPATLMPMVPWK
                     ISVPQTEITPVPAH"
     gene            complement(7802..8530)
                     /locus_tag="VFR82_05650"
     CDS             complement(7802..8530)
                     /locus_tag="VFR82_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU4501117.1"
                     /translation="MSKPLTISSSKRPPVSIDALLDRVPLFNILSLQDRQRLLRELSE
                     TRYGKNDFIFREGEPSEYFHIVKEGTVKCVKSTPDGKECTLKMLMPGDLFCCDAAAFD
                     GANHPGNAQPMGDVSILRMHKSAYFSMLKRNPDAAIEVIKYLGNRLHEAQEKNKILAL
                     DRADQRLAALLTDLASRNGIKERQGLRLPMRLTRQDMANMVGATTETVIRIMSRFKRA
                     RLVSGTANRLVILDFDRLRTLASR"
     gene            complement(8499..10364)
                     /locus_tag="VFR82_05655"
     CDS             complement(8499..10364)
                     /locus_tag="VFR82_05655"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU4501118.1"
                     /translation="MPGTTLSHEQLKALQNTATHLRMESVRSTTAAGSGHPSSCCSAA
                     DIMAALFFSVMRYDPKNPKAPNSDRFVLSKGHAAPVLYAAWAEAGLFPKQDLLKLRTL
                     HSDLEGHPTPRLPFVDMATGSLGQGLPVGVGIALNAKYVDKLDYRTYVLMGDGESVEG
                     SVWEAVEVARQQQLDNLCAIVDVNRLGQSDPTMLQHDMESYRARWSGFGWHAVIVDGH
                     DLPAILGAFEEAARTTHKPTVLLAKTFKGKGISFIQDQPNWHGKPIPQGADMQKALDE
                     LSQQLQPNGAAPIIPRPSTPPTAVPTVKPLPAPTYKVGDSVATREAFGVALEALGGVN
                     PLVVALDADVKNSTYTEKFGKKFPNRFFENFIAEQSMLGSAAGLAACGKIPFAATFAA
                     FFTRAYDFIRMAAISQSNIKLVGTHVGVSIGEDGPSQMGLEDIAMMGAQPGITVLYPS
                     DGTSTYRLMEMAANHKGMVYLRTGRPKSPVLYGSEERFHIGGSKVLRQSANDVLTIVA
                     AGVTLFEALKAHDQLKAKGVSVRVIDLYSIVPIDRTTLLESASATQGRLLTVEDHYAH
                     GGMGDAVLDAVGSEGMRVHKLAVRVIPHSGKPDELIDHYGIGARSIVEATHHIIK"
     gene            complement(10694..11161)
                     /locus_tag="VFR82_05660"
     CDS             complement(10694..11161)
                     /locus_tag="VFR82_05660"
                     /inference="COORDINATES: protein motif:HMM:NF027967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEU4501119.1"
                     /translation="MAMTIPPVRLRQPDHALTLPFKTLRMLMLGLLLVVGSMLTGCGS
                     GEGGPSASATLTWDPVDEVQGGQVQLDLLEGPGIHVVQHGYYVYYGTESQGSSGLCSY
                     PNKVFTSTPSAIVTGLTPNTRYYFAVSAFNGIESECSAEHPADSGDIDPVVIG"
BASE COUNT         2422 a         3038 c         3292 g         2479 t
ORIGIN      
        1 atcatactca catcggcgac ggtaaagaga ttgctgatcg agccgggtgt actgatcgtg
       61 ccgtccttat tgacgtagtc accgacgttg atgttgcgta cagtcacaac gccgtcataa
      121 ggcgcaacga tcgttttgaa ccgaatcagc gcctcgtagt ttctgacgtt ctgttccgac
      181 gctttgacct tcgccgcctc tgctttcgcc tcggcgactt tcaccgagat cgattgctcg
      241 gagactgctt ggttggggcg tagcgcgagc cagcgtttgg ccgtaatatc cgccagtgcg
      301 tacttggcgc gctccgactc gagatccgcc ttggcttgtg catattgcgc atcaagcgcc
      361 ggcgcattga tttcggcaag aatatcgccc ttttttacct gggcgccgta gtccttgtac
      421 cacatcttca cgtagccaga gacctgggca taaatcggag cttcgaacca ggcggtgatg
      481 ttgccgggga gaatgatggt ctcgctggcg tcagccggtt ttggatggac aatagccacg
      541 gtaggcacgg cgtcttcgag cgtcttcacc tgcaatgcag ccgcgtcgct tttggtctca
      601 tagatgcgat agccgacgta gaagaggaac acaatcagca ttgtcagtgc cacgaatttt
      661 tctcggagac ggcgcatcat caggcacgct ccttttggcg agtcattcgc ctgttgtaaa
      721 acatggcgta gacacaaggg acgaacagca gtgtgaagac ggtggcgacg aacaacccgc
      781 caatgacggc gcggccgagt ggggcattct gagaatagcc cgttgccatg gggaccatcc
      841 cgatgatcat ggcagaggcg gtcatgatga ccggacgaaa gcgggtcttg ccggcttcaa
      901 tcgcggctcg cagtgcgtct ccgtgctcct gaattcgctc gcgggcataa gacacgacga
      961 gaatcgaatt cgccgttgcc gtgcccatgg tcaagatggc gccggtcaga gccggctccg
     1021 acagacgcgt atgggtgagg aataacgccc acgcaatgcc cgccagggcg cccggcaacg
     1081 ctgtaataat aatgaacgga tcgagccacg attgaaagtt gaccacgatc aatagataca
     1141 ccaagacgat ggcggccagc agaccgaaga tcagctcgga ataggcctcg tgcatgagag
     1201 aagcctgacc atggatttcg agctcggcgc tgcggggctt ctcggattcc atgcttctcg
     1261 cgatctgttg aacgtccgtc agcacaccgc caaggtcgcg cccttcggcg gaaatataaa
     1321 tatcaaacag gggcatgatg ttcccatgcg tgatcacgcc cggcgtgccc tcggctgaca
     1381 ggtcggccaa attgccgagc agctggatat cattatcttt ggaaggattg gccgaggaat
     1441 cgatcggaat ggtcttcaga ctattcaagc tattgatctg cggctgcggc gtatacacat
     1501 tgatcagata tgacatgccg gttgtaggat cgagccaata gatctggtcg acctgttggc
     1561 ttccggcagt cgtcataagc agattgtcgg ccatgtcctt caaggtccta ttcacaccga
     1621 gtccgaaggt tcggttgcct tcgaccatga gggtcggcgt gcgcatggtc tgctggatca
     1681 ccacgtccac ggcgccgggg acttcgcgga acttgcccac caatttgcgg acgaactcgt
     1741 aattggcata gatgtcgggg ccgttaacct gcacgtcgat cggcgcaggt gcgccgaagt
     1801 tgagaatttt tgcagtcaaa tcggatggct ggaacgtaaa ttcggttccc gggtaacgct
     1861 ccctcaagcc cttgcgcaag atgcgccggt aatcccacac cggagacttc tcatcttcga
     1921 ggaggatcgt gaggtcacaa tcctgagagc cgatcgttgg agttgggatg aaggcgaggt
     1981 tgtgcgggcc gaccgggagg ccacaattac tgacgatgtt ttcgacctgg ccaggcaaca
     2041 actcctcgac gctgttagag acaagagtcg ccaggcggcc tgcgacctcg atacgcgttc
     2101 cgagcggcgc ccgcatatgc atttgaatga ctccagacct gatctcgggg aagtagtcgc
     2161 gtccgaggaa gtagaacagg accgtggatc ccaccgcgaa tgccaacgag attttgataa
     2221 aggcgctgcg acgggcgatc acgcgttcga gcagtgtacc gtagccctcg cggaaccgat
     2281 cgaaacggcg ttcgaacccc ttctgaaagc ggacgaagac gtttcgcgag ggctgcatgc
     2341 gctggtcaag ctgcggcgac atggtgactc ttcatgagat atttcgccat cgttggcacc
     2401 agggtgcgcg acaaaataaa ggaagccagc atggcgatga tgaccgcctc ggccataggt
     2461 ttgaacaagt aaccggcgac accgccgagc tcgaagagcg ggatccatac gatcgcgatg
     2521 cacagggtcg caacgagggt cgggaccacg atctgattcg cggcgtcgat aatagcctgt
     2581 tccagcggtt tgcccatttc gagatggcga tcgatattct caatcatcac ggttgcatcg
     2641 tcgaccagaa tgcccacagc gagcgccaat ccaccaagcg tcatgacatt gatcgtctcg
     2701 ttaagccaat ggaggccgat gatggcggtg aggattgaca gcggaatgga cgtccatacg
     2761 attaccgtcg ccctccacga gccgagcagt agcagcacga tgaggccgac gagggctccg
     2821 gcggttgcca tttcatacac gacgtcagag atcgaatcct tgacgaaggt cgaggcgtcg
     2881 tcgaggagct tgatctttac gccgggagga gacacttgtt cggcccgcgg gatcatcttc
     2941 ttgatcccgt cgaccacatc caaggtcgag gcctcggtgc tcttcatggc aacgatgata
     3001 acggtctgtt tgcccttgac cagcaccgcg ttctgttgca ctcggcccat gagttgcacc
     3061 gtggcaacgt cacgcagata gatgaacgcg ttgccttccc tcttgatcgg gatatcgcca
     3121 aaatcctcta tcttcaatgg tgaggcgttc gtcaagacga tccaatcggt ggatttgatt
     3181 ttaatgtcgc cagagggcag cacgaggtac tgctgcatga ggacattgtg aatatccgtc
     3241 ggggtaagat gacgcgcgag cagtttctgc tgatcgaggt tgaccatcac ctgcatgtcc
     3301 tgacctccgt acgggtgcgg caacacgatc cccggtaccg tcacgagcaa aggtcggatc
     3361 cgcatgtagg ccagattgta aagctccgcc ggcgtcatat tgtcggaact gatttggatc
     3421 atggccaccg gtatcgagga tggcgcgagg cgcatgacca tcggagggga aatatctggc
     3481 ggcagcgcct tgacgacgtt ctgcgcaatc cccacgatat ccgcttcggc cctgcccacg
     3541 tcgacaccat cctggagata aatattcgta atgctactgc cgtagtaaga atgactgtgg
     3601 atatacttaa tgccttccac tgtcgaagtc agaaagcgtt caaacagata ggtgatgcgt
     3661 ccttcgactt gctgcggcaa caggccggtg tagatccaga ccacggaggt gacaggaatt
     3721 gtgatgttcg ggaagacgtc agtcggcatc ttgagcaccg ttttggtgcc cagcaccagg
     3781 atcaagatgg acatgacgac gaaggtgtag ggccggcgaa gcgcaatgag aacgatctga
     3841 ttcataggcc cgccgaactc cggtcagaga ctgtagtgta gagcaacgtg cttcggggta
     3901 gcaaacggct tacctgtctc acgtggcctg tgcgcgaggt gtagtttctt cagaatgagc
     3961 gccatttatc cgctggatat cttcagaacc ccattcagaa tctttctcaa tgagacaaga
     4021 aaatgggaag agagatggcg ttttcgcaca gtgacgactg tgcgaaatct cacaggtccg
     4081 ctgaggcgca acggtcgatc ataacgtcgg tcagacaagg aaccttccaa aatctcgcaa
     4141 ttcaatccat gtcctcttgg gtattcttca gtaactccca tggtaccgat cttgcgtcgt
     4201 cgtaccttgt cttgagcagg tactttacga ataggagcat gatggtgcgg atacggaaaa
     4261 cgagcatggc agtgattctc gcaggcgcga tgatagggtt tggaatggga gtgcctgcca
     4321 gcggtgagac gttgacggtg gccgctcccc cgagcctgag ggcgccattt caagagattt
     4381 tgcggatgtt tgaacaggaa tacggtgtgt cggtcgacgt tcggtatgga ccgtctcata
     4441 cgtttcgccg acagatcgaa cagggagctc ctcttgatgt gtttctatct gactcctggc
     4501 aggaggtaga gacgctacat cagaagggac tgacgctatt cgaaggtcca ctccgatatg
     4561 cgcagacttc aatggtcgtg gtgatgtcct ccgcgtcagc tgccaccgtg atctcagtga
     4621 gtgaggcatc gtcccatgga gccttgcgtg tagctctcgt cgatcccaag gggtcgagcg
     4681 ctggagcgat tgcggtgaag gcgcttaaac agatggatcc cgtagcacat catcatgcgc
     4741 ggcttatcgc aacgcccaac accgatgaag tcgtccgtct ggtcagtgga ggtgaggccg
     4801 atgtcggcgt ggtgtatcgg gccgacgcga tcaacagcgg actggtgcgc ataattgatg
     4861 aagcgcctgc cggccgctcc attccggtcg tgtttggaca agcggtggta tcgacctgtc
     4921 gagaagcctc gcggaaggca ggggagcagt tcttgagttt catgatgagt cctcgtattc
     4981 agaagctctt gctcaagtat ggctttgatg cggtaccaac aaaggccacc gtcggctaag
     5041 gtctctcaaa gacatatatc cattgaatat gttcaggtgg gccatgcggt gatagctctg
     5101 atgcttggaa tctgggcagg tgtcacgccg ccggacccgg gccattctgc actgcctcac
     5161 gcctcaacgc ctcatcgctc aatagtcctt cagtagatgt acctactcat agcctggaat
     5221 cattctttcc atacgggttc ctagtagaag tccgaggttc cgtccgagtt tgactagggt
     5281 atgcccaccc taagctatgg ttctccccat gtgacctcaa aactggtgac ggatatcgtg
     5341 tgaggggaga tcagtggcaa atcgcaggct ctgagaatcg ctcagcatcg cttcgacatc
     5401 actgaacggc cgttcattcg tatggccctc gacagctcac aacagctttc ttagtggaaa
     5461 gggaaaacgc catggctttc ttgccgtttc gtgatcgaac gaggatcgtg ctgttccggt
     5521 ggccggcgat catcgcagga ctttccgttg ccttgggaat tcatatcgtt ctcacggcgt
     5581 ttggcttagg ggtggtcttt cttcaaagtc agtctgcgga gggcggcagc agtatggcta
     5641 cggcggcatt gctgttgtgg agtggtctgt cttgggtcat ggcggcgttt atcggcgggt
     5701 atattaccgc gtgggtggct gatgcctctc gctatgtcga aagccttttc cacggactgg
     5761 tcctctgggg cgcgttgacg ttcatcctga tgttcttgcc accgagtacc atgggcatca
     5821 gcgcggaagc agagtctgcc ttgtcgccgg ccaccatctc atcagtggcc tggtttgtcg
     5881 ccattggggg attgttttcg ctcggcacca cgatttgggg tgccgtgatc ggctcacgtg
     5941 tggttgccga agtggaaacg aaacaaactg gcacctaccg ggccgcgtaa gaatgaccaa
     6001 tggttcaacg ccgaaagaag gataatgaac agaaggcggc cgaaatcgca aaatggatta
     6061 atccgaacga acggattacc attcaatttg acgatcagcg tgacctcagt gctgaggtga
     6121 ccggctgcac ggatcacctc gtatctctct ccctggacac cgccgtcccc cacattcgtc
     6181 agcacgtttc tctgcccttg aattttgtcg aagtctcaga agacttgtcg cattacacgc
     6241 gcgatcctga gcgaccgctc acgcgaaagc gcatgatgct gattgttcaa ggggagcgtc
     6301 cagcagttat ctactgagcg gactcgcgct gggcgctgaa aacgcggagg agcctgccgt
     6361 gatgcaggac ttcatgaccg cagcacggca tccgccgtgt gcgataatca gctggttgag
     6421 gagggcgacc aattcttctt gtgtgcggtg ccggccgtca tggtcgatca ggacatggtt
     6481 gggaatcgca tctgcctgca cctgcgcaat cccctcgagc tgctccagtg cctcggtcat
     6541 cgcctgatgc cactgactgc agtccggacc cgacaatgcg atggtcgcgc gttcaagtgc
     6601 gaatgcgttg cgggctggga gaataacgca aaccgacagg acgcaggcta tgagggcaac
     6661 gtgcgtcacg tatgttggtg gccggtgcct aatgggctgg caccggagtg atttcagtct
     6721 gcggcacgct aatcttccac ggtaccatcg gcataagcgt agcaggcttg gcttcaggcg
     6781 aagccacgag cacagcaatt gcgcctctca atgccgcggt taacgagtgg gtcacaagcg
     6841 gatacgcccc ggcggactcg gcaacaacgt cgaaaatcgc cgcgctacca ggcccgacca
     6901 cgtaggtttg gactcccgtc aatttgttgg ccggattgcc gctttcatac acattgtccc
     6961 aaatctcacc gatcggatga aatgccgaga attcatttgg gccggcgtta acgaagaaaa
     7021 tccgtactcg ttccccaggt ttcgcatcga gtttgcctcc gcccgaaacg aacgggtgat
     7081 atttgaagag gccgccgttg aacatgacgc cgtcgaactt ccgatcgaac atggcctgaa
     7141 catcgtcggg attcttgaag tactcagatt ggatcaagac atattcccgg tccgccttcg
     7201 gccaggcact ggcgttcttg ggatcgacga tgatggcgcc gaacatgccg cgggcgacat
     7261 gttgaatcat cggaggtgcg ccgcagtgat aaaagaagat gccgggtttc ttggcgacga
     7321 aggtgtagct gattgtttca ccggcgttga tggctttgta gttgttaagg aagtcaatct
     7381 ccgcggcatg aaaatccatc gcgtgcggat acttgtttgt cgccggattg gtcagcgtaa
     7441 acttgaccgt gtctccctca gtgaccctga cgactgggcc tgggacttgt ccatcgaatg
     7501 tccaggctgc atacttctcg ccgctgccct ctatcacgac ctccgtttcg acagctttca
     7561 tggtgatctc atgagttttg gcgacaaccg tttccggcga tgtcgcactc agggctattg
     7621 agatgctcag ggctagaagg gcaagaactt gagatgggcg cattgacata gacggggtcc
     7681 tcctcagggc aacgggagat agagtcacga tacagtcagc catccttaga aattaacctg
     7741 ggcgagcata ccgaagtgtc actgagcaaa acatgaccaa tgtcatgttt tgcgggagag
     7801 atcagcgtga ggcgagcgtt cttaaacggt caaagtcgag gatgacgaga cgattggcag
     7861 ttcccgagac gagacgagcg cgcttaaagc ggctcatgat ccggatgacg gtttcggtcg
     7921 ttgctccgac catattcgcc atatcctgcc tggttaatcg catggggagt cgaagtccct
     7981 ggcgctcctt gatcccgttc ctgctcgcga ggtcagtcaa gagtgccgcc agcctttggt
     8041 ccgcacggtc caacgcgagt attttgtttt tttcctgcgc ctcgtgcagc ctattgccga
     8101 gatacttgat aacttcgatt gcggcatcgg ggttccgttt cagcatgctg aagtaggcgg
     8161 acttgtgcat gcgaaggatg ctgacgtcgc ccatcggctg tgcgttgccg ggatggttgg
     8221 cgccatcgaa cgccgccgca tcgcaacaga acaaatcccc cggcatcagc attttgaggg
     8281 tgcattcctt gccgtccggg gtggacttca cgcatttgac cgtaccttcc ttgacaatat
     8341 ggaagtactc ggacggctcg ccctcccgaa agatgaagtc attcttgcca taacgggttt
     8401 ctgagagttc gcgcaacaac cgctgacgat cctgcaggct caggatgttg aaaagtggca
     8461 cacggtcaag cagcgcgtcg attgagacgg ggggccgctt acttgatgat atggtgagtg
     8521 gcttcgacaa tggatcgcgc tccgatgcca tagtggtcga tgagctcgtc cggttttccg
     8581 ctatgcggga tcacgcgcac ggcgagctta tggacgcgca tgccttcgga tcccaccgcg
     8641 tcgagcacgg cgtcgcccat tccgccatgc gcataatggt cctcgactgt gaggagacgc
     8701 ccctgggttg ccgaggcgct ttccaataag gtcgtgcggt caatcggcac gatgctataa
     8761 aggtcgatga cgcgcaccga gacacccttc gcctttagct gatcgtgagc cttcagcgct
     8821 tcgaacagcg tcacccctgc cgcgacaatc gtcaggacat cgttcgcgct ttgccgcagg
     8881 accttggagc caccgatgtg aaaccgctcc tccgaaccat aaagaaccgg cgatttgggt
     8941 cgtccggtgc gcaaatacac catccccttg tggttggccg ccatctccat caaacgataa
     9001 gtggaggtgc cgtccgaggg atacagcacg gtaatgcctg gctgagcgcc catcatcgcg
     9061 atgtcttcca gacccatttg tgaaggcccg tcctcaccga tgctgacccc gacgtgggtt
     9121 ccgacgagct tgatattgga ttggctgatc gccgccatgc ggatgaaatc atacgctcgc
     9181 gtaaagaagg ccgcgaatgt tgccgcgaac gggatctttc cgcaagcggc caaaccggcc
     9241 gctgagccaa gcatgctctg ttcggcaatg aagttctcga agaacctatt ggggaacttc
     9301 ttgccgaact tttcagtgta cgtggaattt ttcacgtcgg cgtccagcgc gacgaccaac
     9361 ggattgactc cccccagcgc ctcaagcgcg acgccaaacg cttcacgtgt ggcgacagag
     9421 tccccgacct tataagtagg ggcagggagg ggcttgaccg tcgggacggc agtcggaggg
     9481 gtggacggcc gcgggataat aggcgctgcg ccatttggtt ggagttgctg cgacagctcg
     9541 tcgagcgcct tttgcatgtc ggcaccctga gggatcggct tcccatgcca gttcggctgg
     9601 tcctgtatga aggagatgcc tttgcccttg aacgttttgg cgagaagcac cgtgggctta
     9661 tgggtggttc gagccgcctc ttcgaacgcg ccgagaatgg ctggcaggtc gtgaccatcg
     9721 acgatcaccg catgccatcc aaaacctgac caacgcgctc gatagctttc catgtcgtgt
     9781 tgcagcatcg taggatcgct ttgaccaagc cgattgacat cgacgatggc acacagattg
     9841 tccaactgct gctgtcgtgc gacttccacc gcctcccaga ctgagccttc caccgattcg
     9901 ccatcgccca tcaacacata ggtacgataa tcgagtttgt cgacatactt tgcgttcagc
     9961 gcgatgccaa ctcccacggg taatccctgt cccagggaac cggtggccat gtcgacaaat
    10021 ggaagccggg gagtgggatg cccctccagg tcggagtgga gcgtgcggag tttgaggagg
    10081 tcctgtttgg gaaagagtcc ggcttcggcc cacgcggcat acagcacagg cgcggcatga
    10141 ccttttgata gcacgaatcg gtcgctgtta ggggctttcg ggttcttcgg atcatagcgc
    10201 atgacagaaa agaagagggc ggccatgatg tccgcggcgg agcagcagct cgaaggatga
    10261 ccgctgccgg cagcggtggt cgatcgcacg ctttccatgc gcaaatgggt cgccgtgttc
    10321 tgtagggcct ttagctgctc gtgagataat gtggttcctg gcatgtgctc tccttggtgt
    10381 cagagcgtaa tcgcaaaaac aaccggaccg ccctgagtgg acggctcctc ttgcgtgaaa
    10441 aagtgaagct aacaaaccga gggcgaagga gtcaacgaag acgaacggag tccgggcgag
    10501 acaacaggct ccgcgtgatg gagacaataa actggcgcat gagtcgtggt ccgatatgag
    10561 gccgtctgga agaaaatcaa tgactgcgac cgtccccacg gatccaaacg atctcagcgg
    10621 taaagcgccc gacctgtcaa cgaggtcgtc gaggatatca acaacctcgt tgcaacgcct
    10681 gcgctctgac ggatcagcca atcaccacgg ggtcaatatc accgctgtcg gccgggtgtt
    10741 cggcggagca ttcactttcg ataccgttga aggcactcac ggcaaagtag tacctcgtgt
    10801 tcggtgtcag ccctgtcacg atggcggaag gcgtcgatgt aaataccttg ttgggatagg
    10861 agcagaggcc tgaagagcct tgtgactccg ttccatagta cacatagtat ccatgttgca
    10921 ccacatgtat tcctggccct tcgagaagat ccagttggac ttgtccgcct tgcacttcgt
    10981 caaccggatc ccacgtcaaa gtcgcagagg ctgagggccc cccctctccg gagccgcaac
    11041 cggtgagcat cgaaccaacc accagaagta ggccaagcat cagcatcctg agcgtcttaa
    11101 acggaagtgt gagcgcatga tcaggttgcc gaaggcgcac aggtggtatg gtcatggcca
    11161 tgtggtcagc tcctttatga acgtctcgag ttggtaaaat tcggactcga ctggatgtga
    11221 gtgaagaaac c
//
LOCUS       DASTQY010000175         5511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1193711, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000175 DASTQY010000000
VERSION     DASTQY010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193711"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..578)
                     /locus_tag="VFR82_05665"
     CDS             complement(150..578)
                     /locus_tag="VFR82_05665"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HEU4501120.1"
                     /translation="MLPDVARRVLTTCGIIVFGLNLAACNTTKATIDTTVNFFSSTTP
                     NDLFTVDGLVKQEQKINLFAGVAYENLRQEAAAGSGQYVTSLAALYGVPEANHNAFGK
                     VLQQRHAELFATDLVQDNRAHLRMVSVLNRVMTQEPSLMQ"
     gene            complement(891..3491)
                     /gene="uvrA"
                     /locus_tag="VFR82_05670"
     CDS             complement(891..3491)
                     /gene="uvrA"
                     /locus_tag="VFR82_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU4501121.1"
                     /translation="MSHPAISSLAGILETPVPTRDRFECYNPARMGNSIIIKGAREHN
                     LKNIDVEIPRDKLVVITGLSGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLEQMGKP
                     DVDSIEGLSPAISIEQKSTSHNPRSTVGTVTEIYDYLRLLFARVGRPYCFQCGEEITA
                     QTVQQMVDAIGALPEGSKFQVLAPIVRGRKGEYRKELLEMRRAGYVRGRIDGKPVDLG
                     DDLTLDKQKKHTIEIVVDRLVMKAGDALARRLADSVETSLKLTGGLVGVLTEEGKTLL
                     YSEKLACIRCGVSYPEITPRVFSFNSPHGACPACDGIGYAMPPDATDDQDLTLLGACE
                     ACHGARLRAESLSVKIARQSIADVTSRSVRAAAEFFASLTFTEREMVIAHRILKEIRE
                     RLGFLVNVGLDYLTLDRAAATLSGGEGQRIRLATQIGSGLVGVLYILDEPSIGLHQRD
                     NRRLLQTLLRLRDLGNTVVVVEHDAETMMAADHILDMGPGAGAHGGHVIAQGTPQQIM
                     SDARSLTGHYLRGVQSVSVPSRHRKPKGFLSVVGAHKHNLKNVTAKIPLGLLTCVTGV
                     SGSGKSTLVLEVLFHSLSQLLYHKKPKIDGCINLHGVLALDKVIDIDQSPIGRTPRSN
                     PATYTGLFGYVRDLYSNMPESRVRGYTPGRYSFNVKGGRCEACQGDGLIKIEMHFLPD
                     VYVTCEVCKGQRYNRETLEILYKDKSIADVLNMTVEEALAFFENIPLVKSKLQTLHDV
                     GLHYVKLGQSATTLSGGEAQRVKLSRELSKRATGRTLYILDEPTTGLHFADVQRLLDV
                     LDRLVEAGNTVLVIEHNLDVIRNADWILDLGPEGGDRGGEIVAEGPPADIAKAKRSYT
                     GQVLKEAGVR"
     gene            3589..4347
                     /locus_tag="VFR82_05675"
     CDS             3589..4347
                     /locus_tag="VFR82_05675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4501122.1"
                     /translation="MTEQQAPDVPARAISTWTGSAREQAVQRMFTAIAGVYDLNNTLL
                     SFGLHYRWKTITASYIPTREQGTALDVGAGTADLALLLEPRMGVGGRVIASDLNHAML
                     VEGLKKVNGKGLTGKITCLQVNAEHLGFDDDSFDAVTTGFCMRNVGDLARAVGEIRRV
                     IKPGGTFACLEFSRPVFGWLRALYDWYSFKLLPWVGTKVARDTTGVYEYLPASIRTFP
                     DQERLCQMLREAGFRQVSYKNLSGGIVAIHIARK"
     gene            4411..>5511
                     /locus_tag="VFR82_05680"
     CDS             4411..>5511
                     /locus_tag="VFR82_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU4501123.1"
                     /translation="MNSILYIFLPCKKVYPIGITYLADFVHRRKPEVRQRILDLSLHP
                     VSQRSRAIRDAMSDFQPDLVCFSWRDIQIFSPHEGDSSLEHAFNFYFASNPIKRVAAS
                     FAGLKQLYRYYSHIYTVLSYPWLIRKEFPKSQIMIGGGAFTAFADQLIEKLPEGTIGL
                     LGEGEDAILKLIEGRSIEDERYIIKEGKQVKKGRQGSPALLDALTVDLPYLTSIFPQY
                     REYIGESIGVQSKRGCPYDCAFCLYPYIEGKRVRYRPAEMVVRDISQHYHQWGARRFW
                     FTDAQFITGKEAYPQCTEILERIISEKMQLEWSGYIRTSLITPELAKLMVRSGVGDLE
                     VAITSGSQEVLNTLHMGFKLERLYDGCRYLAEA"
BASE COUNT         1161 a         1619 c         1506 g         1225 t
ORIGIN      
        1 gaaatctcac tcggtgtgtg gttatcgaag gatgcccaga aaccacagca cgaccagcag
       61 gctcactggc actccgagca gccacaaaat gacgcctttc cccatagctc ccatcttgtt
      121 cctccgtctg agttcctact cggagttttc tattgcatta acgagggttc ttgagtcatg
      181 acccggttca acacgctgac catgcgcaga tgggcgcggt tgtcttggac caagtcagtt
      241 gcgaataact ccgcatgccg ctgctgcaag accttgccga aggcattatg gtttgcctca
      301 ggtacgccgt acagtgcggc caacgacgtc acatattgcc cgcttcctgc tgcggcttcc
      361 tggcgcagat tttcatacgc cactccggcg aacaggttaa tcttctgctc ctgcttcacc
      421 aggccatcta ccgtaaacag gtcgttcgga gtcgtgctcg agaaaaagtt gactgtcgtg
      481 tcgatcgttg ccttcgtcgt gttgcatgcg gcaaggttca atccgaacac gatgattcca
      541 caagtggtca aaactcgacg tgccacatca ggtaacatcg ctcctccttt cgggtaatag
      601 cgtcggtaga aaaagcggac cctacaggac cgctcattcg cccctaggcg tacgtgcagt
      661 cgaaactccc agattctgga gtaagcaagg gacgggccga acttgtaagc acgcgtcacc
      721 gctgaatgtc acgacaaacc agggtgtctc cgcaggaata gcctccaatc gccgccgttc
      781 ggccctggta gcatggacaa gttgtccctt ttgcgggaca ggttggatga ttgattacac
      841 aatgcctggg aggaactgaa gagctttcgt atgggtgaac aacaggaggc tcatcttact
      901 cctgcctctt taagaacttg gcccgtgtaa gaacgcttgg cctttgcaat gtcagcgggc
      961 ggcccctcgg ccacaatttc accgccgcgg tctcctcctt cagggccgag atcgagaatc
     1021 caatcggcgt tcctgatcac gtcaagattg tgctcaatca ctaggaccgt gttgcccgct
     1081 tcgacgagcc gatccaagac atcaagcaac cgctgcacat cggcgaagtg cagtccggta
     1141 gttggttcgt cgagaatgta caacgtccgt ccggtggcgc gcttggacag ctcgcgagac
     1201 aattttaccc gctgagcctc tccgccggag agggtggtcg ccgactgccc gagcttgaca
     1261 taatgcagac cgacatcatg cagtgtctgc aatttgctct tgaccaacgg gatgttctcg
     1321 aaaaatgcga gcgcttcctc caccgtcatg ttcagcacat cggctatgct tttgtccttg
     1381 taaaggatct ccagcgtctc gcggttgtag cgttgccctt tgcacacctc acaagtcacg
     1441 tacacatcgg gcagaaaatg catttcaatc ttgatcaatc cgtcgccctg gcaggcttcg
     1501 catcggccgc ctttgacgtt gaaactgtac cggcctggcg tatatccccg cacgcgcgat
     1561 tccggcatat tggaatacag atcgcgaacg tatccgaaca atccagtata agtggcgggg
     1621 ttcgaacgtg gcgtccgccc aatcggcgac tgatcgatgt cgatcacctt gtccaacgca
     1681 agcacaccgt gaagattgat gcatccatcg atcttcggtt tcttgtgata gaggagctgc
     1741 gaaagcgagt ggaacaacac ctcgagcacc agagtgctct tgcccgatcc cgaaacgccg
     1801 gtcacgcagg tcaagagccc caagggaatt tttgccgtga cgttcttgag attgtgctta
     1861 tgcgcgccaa ccacggagag aaaacccttc ggtttccggt ggcgcgacgg caccgagact
     1921 gactgcacgc cccgcaagta gtgccccgtc agggagcggg catcgctcat gatctgttga
     1981 ggcgtgccct gtgcaatgac gtggccgcca tgcgcccctg ctccggggcc catatcaagg
     2041 atgtggtcag cggccatcat cgtctcggcg tcgtgttcga ctacgacgac ggtgtttccg
     2101 aggtcgcgca atcgcaagag cgtttgtagc agccgccggt tatcccgttg gtgaagtccg
     2161 atggacggtt catcgagaat gtagagaaca ccgaccagtc ccgagccaat ctgcgtcgcc
     2221 aatctgatgc gctgtccttc tccaccggac agcgttgccg ccgctcggtc cagcgtcaga
     2281 tagtccaatc ctacattgac cagaaagccg agccgctctc tgatctcctt taggatccta
     2341 tgggcaatca ccatctcgcg ctcggtgaat gtgagggacg caaagaattc ggccgctgct
     2401 ctgaccgacc ggctcgtgac gtctgcaatc gactgcctgg caatcttgac ggagaggctt
     2461 tcggctctga gcctggcacc atgacacgcc tcgcaggctc ccagcagtgt caggtcctgg
     2521 tcgtccgtgg cgtccggcgg catcgcatac ccgatgccgt cgcaagctgg acaggccccg
     2581 tgcggactat tgaaggagaa gacgcgcggc gtgatctctg gataactgac gccgcacctg
     2641 atgcaggcga gcttttcact gtacaaaagg gtcttcccct cttccgtcag cacgccaaca
     2701 agcccgccgg tgagcttcag cgaggtttcc acggagtcgg ccagccggcg cgccaacgca
     2761 tcaccggcct tcatcaccaa gcgatctacg acgatctcga tggtgtgttt cttctgttta
     2821 tcgagcgtca ggtcgtcgcc cagatccacc ggcttaccgt cgattcgccc tcggacatag
     2881 ccggcccgcc gcatttccaa cagctctttt cgatattcgc ctttccggcc tcggacaatc
     2941 ggggccaaga cttggaactt acttccctcc ggcaaagccc cgatggcatc gaccatctgt
     3001 tgaaccgttt gagcggtgat ctcctcgccg cattggaagc aatagggacg acccactcgg
     3061 gcaaacaaca agcgcaaata atcataaatc tccgtgacgg tcccgacggt cgaccgggga
     3121 ttgtggctcg tacttttctg ctcgattgag atggccggcg agagaccttc gatcgaatcg
     3181 acgtccggtt tgcccatttg ctccaaaaac tggcgggcgt aggcggacag cgattccaca
     3241 tagcgccgct gtccctccgc gtagatggtg tcgaaggcga gcgaggactt acccgaaccg
     3301 ctcaaacccg tgatgaccac cagtttatct cgcgggatct cgacgtcgat attcttgaga
     3361 ttgtgttcgc gtgcgccctt gatgatgatc gaattgccca tacgggcagg attataacat
     3421 tcgaatcgat cacgtgttgg aaccggtgtc tcgagaatgc cggctaggct ggagattgca
     3481 ggatgtgaca acgacgctcg ccggcttttt gaagcatcct gcccgttcct tgacaaagcc
     3541 ggaaaccggg tgctaccacc cattcgcatg cgttcagtgg aggagtccat gaccgagcag
     3601 caggcgccgg acgttccggc acgcgccatt tccacctgga cgggctccgc acgagagcaa
     3661 gcggttcaac gcatgttcac ggccattgcc ggcgtgtacg atctcaacaa tacgctgctg
     3721 agcttcggac ttcattatcg ttggaaaacg atcacggcgt cctacattcc cacccgagaa
     3781 caaggcaccg ccctcgacgt gggagcaggc acggccgatc tggccttgtt actcgagcct
     3841 cgcatgggtg tcggtgggcg ggtcatcgcc tcagacctca accacgccat gctcgttgag
     3901 gggctgaaga aggtgaacgg caaaggcctc acgggaaaga tcacatgcct gcaggtcaac
     3961 gctgagcatc tggggttcga tgacgattcg ttcgatgccg tgacgactgg attctgcatg
     4021 cgcaatgtcg gcgacctcgc ccgcgccgtc ggtgaaatcc gccgggtgat caaaccgggc
     4081 gggacatttg cgtgtctgga gttttcgcgg ccggtctttg gctggctacg cgccctgtat
     4141 gactggtatt ccttcaaact cttgccgtgg gtgggcacca aagttgcccg tgacaccact
     4201 ggagtctatg aatatttgcc cgcttcgatc cggacgttcc ccgaccagga acgactgtgc
     4261 cagatgttgc gcgaagccgg tttccgtcag gtatcctata agaacctatc gggcgggatt
     4321 gttgcgatcc acattgctag aaaataaggc cggacgattc atgcgtccgc ctacaaacca
     4381 gatggctcat tcaacgctcg ccggtgctgc atgaactcta ttctctacat cttccttccg
     4441 tgcaagaagg tctatccgat cggtatcacc tatctggcgg actttgttca ccggagaaaa
     4501 ccggaagtcc gtcagcgcat tcttgatctc tccctccatc ccgtctccca acggagcagg
     4561 gccatccggg acgcaatgag cgatttccag ccggacctcg tctgcttttc ctggcgggac
     4621 attcagattt tttcaccgca cgagggggat tcttctctgg agcacgcatt caatttctat
     4681 tttgcgagca accccatcaa gcgggtggcg gcctcattcg ctgggctcaa gcagctgtat
     4741 cggtattaca gccatatcta tacggtcttg tcctacccgt ggctcatccg gaaggagttc
     4801 cccaagagtc agatcatgat cggaggcggg gctttcaccg cctttgcgga ccagctcatc
     4861 gagaagttgc ccgaagggac gatcggactc ctgggcgaag gcgaggacgc gattctcaaa
     4921 ctgatcgaag gccggtcgat cgaagacgaa cgctatatta tcaaagaagg caagcaagtt
     4981 aagaagggca ggcagggctc gcccgccttg ctcgatgccc tgaccgtgga tctgccgtat
     5041 ctgacctcta tttttcccca ataccgtgaa tacattggcg agtccattgg tgtccagtcg
     5101 aaacgcggct gtccctatga ctgcgcattt tgcctctacc cgtacattga agggaaacgg
     5161 gtccggtatc gtccggctga aatggtcgta cgagacatct cccagcatta tcatcagtgg
     5221 ggagcacgac ggttctggtt taccgacgct cagtttatta ccggaaagga agcctatccc
     5281 caatgcactg aaatcctgga acggatcatc agcgaaaaaa tgcaactcga atggtcgggg
     5341 tacatccgga cgtcgttgat cacccccgaa ctggcgaagc tgatggttcg atctggtgtg
     5401 ggggatttgg aggttgcgat cacctcaggt tcacaagagg tgctcaatac tcttcacatg
     5461 ggattcaagc tcgagcggct ctatgacggc tgccgctacc ttgccgaggc a
//
LOCUS       DASTQY010000176        15612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_47785, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000176 DASTQY010000000
VERSION     DASTQY010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 15612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15612
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47785"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /gene="ispD"
                     /locus_tag="VFR82_05685"
     CDS             <1..543
                     /gene="ispD"
                     /locus_tag="VFR82_05685"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU4501124.1"
                     /translation="PATDLQYCRRDIVEAHGFTKVLHVVAGGQERQDSVRQALAVVDE
                     RVDIVVIHDAVRPCVTELMVKQVVEAAKAEGAAIIALPMRDTVKQVGPGGLIERTIDR
                     KPLWLAQTPQAFRRDWLQDAHRKAHAEGLAATDDAYLLEWMGHPVRVLEGSGENIKVT
                     RPEDMILGEAILSSRATHGA"
     gene            574..1047
                     /gene="ispF"
                     /locus_tag="VFR82_05690"
     CDS             574..1047
                     /gene="ispF"
                     /locus_tag="VFR82_05690"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEU4501125.1"
                     /translation="MRIGYGYDVHPLGPGRKLILGGIDIPHSKGLLGHTDSDVLVHAV
                     CDALLGAMGEGDLGRHYPSSDSKYKGMSSLKLLEDVMTKLKVKGYRVGNIDTVIVAQA
                     PRLGPHLAAMQKKMAEAAGIDPNQVNVKVKSGEGLDAVGKEEGMIAHAVCLIEPA"
     gene            1067..1717
                     /gene="cysE"
                     /locus_tag="VFR82_05695"
     CDS             1067..1717
                     /gene="cysE"
                     /locus_tag="VFR82_05695"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU4501126.1"
                     /translation="MFKAIRRDLKAVFDRDPAATSSLEVIFTYAGFHALIAYRFSHWL
                     KSYGVPLVPRVISQVARWLTGIEIHPSARIGTGFFIDHGMGVVIGETAEIGDFVTLFQ
                     GVTLGGTGKERGKRHPTLGNHVVVGAGAKILGGITIGDNVKIGANSVVLKNVAANSTV
                     IGVPARVIKTQGERLPDATMDHINLPDPISDRFLALEQELIELRKKLENRDDSPPR"
     gene            complement(1822..2598)
                     /gene="truA"
                     /locus_tag="VFR82_05700"
     CDS             complement(1822..2598)
                     /gene="truA"
                     /locus_tag="VFR82_05700"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU4501127.1"
                     /translation="MPTVRLILEYDGSRYAGWQRQPDQPTIQEAVETALFQLTQETVA
                     VIGAGRTDSGVHAIGQVASFRIERDWTPREWMRGFNARLPEDIAVRSADLVPNDFHAR
                     YAARGKLYEYRILNRPQRPALDRAYVWHVHKPLDHTAMEQAAIHLVGSHDFSSFEGTL
                     TDNEDPVCHLRQLSLTRQEDQLTIHAYADRFLKHMVRAIVGTLVEIGHAKRTPESLRM
                     ILAAKNRTAAGRTAPPQGLFLVRVDYEPQLDSVPANPPTT"
     gene            complement(2626..3906)
                     /locus_tag="VFR82_05705"
     CDS             complement(2626..3906)
                     /locus_tag="VFR82_05705"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU4501128.1"
                     /translation="MPATVSRVAALILVLGLLSTEDFLANGAWLGAAHAAPQDQRRDH
                     QRNSKRSRNSNVSLSPNTIPLAKIQNLRTAFLPDSLRLVLDLDRKTQVKKHRPAQEDG
                     VVVEIPHAALTKSAQAKIAQGVLEGRFVVKQTVGDSVTVALPSGSFETYSLLSLTNPP
                     RLVVDVIPSGLPPSGSVQDPTDSSMFSAPPPQPVAPRAKSYKTIVIDPGHGGRDPGAH
                     GQRGTEEKDITLKVGLKLRDLLRKDPNVRVLMTRERDVFVDLEDRARFANGNEADLFL
                     SIHVNSHPQRSVRGIEIYHFGEAKDQRALELAARENGTPLSSTGVGWEYLVADLLTSK
                     KIEASLELAWNTKEAMITRLNGDYALVDHGVKTAPFYVLRFTSMPSILAEIAYISNAA
                     EEDLLRTAGFTTRIAESLHEGVTAFLAPSKTPAR"
     gene            4043..4567
                     /locus_tag="VFR82_05710"
     CDS             4043..4567
                     /locus_tag="VFR82_05710"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEU4501129.1"
                     /translation="MHVIIDGYNLLAVVRPLGGANLYSDTAREALLRLLGGYRHRKGH
                     ALTVVFDGWQRGQPIENREHRAGVHVIYSKRGERADQVIQRLAREYGAQCAVVSSDHE
                     IVNSAQAHGALVISARAFADKLHTQGSSETSVPYKELDPGEDPAERRRPEKKGNARKL
                     PKSLRRRSRQLKGF"
     gene            complement(4554..5324)
                     /locus_tag="VFR82_05715"
     CDS             complement(4554..5324)
                     /locus_tag="VFR82_05715"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU4501130.1"
                     /translation="MLIDTHTHLDDARFDGDRDAMIERARQAGIEAFVTIGCDLATSR
                     SAVALADQHPDVFASIGVHPHEVKHVEDAWYSDFRRLAQHRKVVAYGEIGLDYHYNHS
                     SPQSQRQRFREQIQLARELRLPVIIHTREAQEDTITILKEEKAAEVGGVFHCFSGDAW
                     LAKDALDLGFYLSFSGILTFQNAAMLRDIAKQTPLDRVLIETDCPYLTPVPHRGKRNE
                     PAFVPHVAKQLVAIRPDLSYEDIERTTSANAKRLFKIP"
     gene            complement(5448..6506)
                     /locus_tag="VFR82_05720"
     CDS             complement(5448..6506)
                     /locus_tag="VFR82_05720"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU4501131.1"
                     /translation="MNRPLDNQHVIEIKALPSPRAIKTKLPITDDVAALVVETREAIR
                     RILHGQDRDRLLVIVGPCSIHDPEAAFEYAVKLKPLSDAVRDRLLIVMRTYFEKPRTT
                     VGWKGLINDPHLDGTCDIATGMELARTTLLKINQSGMPCATELLDPISPQYIADLISW
                     TAIGARTTESQTHREMASGVSMPVGFKNGTEGSLHVAVNAMTSARTPHHFVGINADGQ
                     TSIIKTMGNPDRHIVLRGGGGKTNYDAEHVAKAEAAVAGEGIARSIMIDCSHDNSSKD
                     HTRQALVAREVLRQFRDGRHSIMGLMLESNLNPGKQTWKQGVALAHGVSITDACLGWD
                     DTAALLNELAESIVARPA"
     gene            6667..6969
                     /locus_tag="VFR82_05725"
     CDS             6667..6969
                     /locus_tag="VFR82_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501132.1"
                     /translation="MSSPVQALSLSLILLILVFAPFAAAQDIHHELAAADTDGHEHSD
                     TDLCQWVQHHTSGSVDIGGPICSAWQSTGQYDVPPHSILFSLDSTTVGPSRAPPHS"
     gene            7062..9473
                     /locus_tag="VFR82_05730"
     CDS             7062..9473
                     /locus_tag="VFR82_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4501133.1"
                     /translation="MRREGSCYCCGVLLMTGAGWLISSETLLNNAAAKDSGQTIVGTV
                     QNQDLRRVDQATVQVRDQEGNMVAQTVTNQGGEFRVVVPQLGTYSISAVRDTDRSEYV
                     VVKIGAEQPAPVSLTLTATREIALEIVSPLPAIQYKASSETYQLSRKEIETLPRGNNN
                     DVHDVLLTVPSVVYGGLKQTHIRQDHANQQYRIDGIPIPDTVSGAFADIVPPRAWERA
                     DIILGGMEAQYGNKTALVVDITSKSGTTPGFGSAQVFGGSNQTVNPSFEYGGAIGEKV
                     RFYVLNSYVTTSRGIEPPTLGKTNHHNQSEKNNTYLRGDYQHDNRNNFSWIFLNSVAK
                     HQIPTVPDLAPNPDVLALLPGFTPTASQSVDQFQKEDSQYSQLVWRHDVNASNLFGLG
                     LYFRRGVADFRTDPSNALAYTEDVSSAQTANQRRVAYSGGVRFDYTWIPDSRHLVKTG
                     FQLDYTRANNQSQIFAFDTSSGAPAGPVLSLNASNQNIQTRQELWVQDQWSPTEDWTF
                     NLGVRGDAIQGFYNEGQISPRVGVTYKYNRSNVYHAYYGRLFTPPNVEQVAFAQLNTQ
                     NTTAAPDNPTGFRPRAERSHYFEVGSYHALHRNATLELTGYYKLAHYLSDAGQFGSTP
                     LLNFFAFERGWQRGIDGALKVQFTENFTGRGNVAWGQCKGYGLQSGQYLLDQVEINDI
                     NSTGGVFCDHSQFVTSSAVLSYRLWERTMFTGQMLYGSGLRTAEPGAKTNSSHENSYT
                     VYNASITHTILLPWHGQKMLVGFDVINLLDQHYFINRGEGSIGLGIAHAGMPRSYFFR
                     AQWFF"
     gene            9558..10010
                     /locus_tag="VFR82_05735"
     CDS             9558..10010
                     /locus_tag="VFR82_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU4501134.1"
                     /translation="MTHTSTSFVALMLCVISLAVGSLPICTYAAAGDEATHESDHQED
                     VLELPEVHVHGLSLNKDQQIGPVPKSTPWPGIPPALEGSVIDDWMKARMLVSKEAKVT
                     VVILEPCRHRELTAAGIAALAKWTFEPQMQGDVPIDGELTVRLHFHTK"
     gene            10059..10637
                     /locus_tag="VFR82_05740"
     CDS             10059..10637
                     /locus_tag="VFR82_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU4501135.1"
                     /translation="MSWDVFLTRSINGLAGKHAWTDWLFLTLSNPDMLWLPGIFLGTY
                     WLWRSPREALIGGPILGVSIGILDFIGAQLKYLAARPRPCVSMADLIQLQACGKTFSF
                     PSNHAVNTATAAAFFQVLYPRSGWISWPIVGLIGMGRVYIGAHYVSDVIGGWMIGGLC
                     GAGIAWLLLQGPRFRQRVLSPIVSTQADSSVQ"
     gene            complement(10583..11554)
                     /locus_tag="VFR82_05745"
     CDS             complement(10583..11554)
                     /locus_tag="VFR82_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU4501136.1"
                     /translation="MTQPAVPFYILCGSLGAGKTTLLMRLLEHWKRLGIRAGVLMNEA
                     GEISIDGPRVGTIAEQVMNLAGGCVCCDTKEDLSWGIAQLVREYRSELIVLECSGLAD
                     PAEVVDGVTDLYTARLTKLERIIALLQPIANDQSPSSTFVTEQAIRYADELILNKRDL
                     YVPGHWEQFKASIVARNAHARVWETTHARIDAAALLSGHDRSHSAEPTNVEFGRSRPT
                     SHLRAGYHPMATTVRLSGTLNRDRFLSWAKTLPKEIERAKGFLRFTQEPELQEFQYAP
                     PGEPTILPVMLLDEPEKAIVLIGRGYDVDRYCTELSACVETIGDNTR"
     gene            complement(11564..12502)
                     /locus_tag="VFR82_05750"
     CDS             complement(11564..12502)
                     /locus_tag="VFR82_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU4501137.1"
                     /translation="MTGRAAYTHLRSRLHLALGLNAIVIVAEFVGGILINSIGLIGDA
                     GHNLIDQGALFAALYAHILTARPATEARTFGYHRAGIVTAFLSSFVLLLTALGITIVG
                     VQRLIHPVSVDGSGVMAIAAISFAANLGIALLLQHGAKDDLNIRSAFWHMLADAWVSL
                     GVILSGAAIVATGWTVLDPLISILLVGAIIRGALPIFRESLEVLLESTPPGVSASHIA
                     ATIEAIPGVDNVHDLHVWAIEPRLVMLTCHVLIDADDTAVTNELLRDIRCRITSDFGI
                     THMTIQLETHCAHKDAVHCDLMALASRQDDRLHAHP"
     gene            complement(12566..13420)
                     /locus_tag="VFR82_05755"
     CDS             complement(12566..13420)
                     /locus_tag="VFR82_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501138.1"
                     /translation="MTRLRLLACAWVLTLLIPPPCWAIPAEGEPPPDPRLNAPKPTTI
                     LVRVVAHGAMVIGREVGGARVTITDVETKQVLATGIQQGEAGDQNQIMRTPHLMEEPV
                     YSARPAAAFTTTVELRRPTLVEITAEGPLAHPASMQHASQTVLLIPGQDLTNDGIVLH
                     LYGYIVQIEHPKQGQALMATDDVTLRASVKTLSGTLVRPHGDWDSRKIRIYGELLIGD
                     RIVERLQLFFSNETGTFEAPFFVPNPHEAPDGILLRVVATDLATGNSGAGTAACLIQK
                     PLRSSTNP"
     gene            complement(13417..15420)
                     /gene="metG"
                     /locus_tag="VFR82_05760"
     CDS             complement(13417..15420)
                     /gene="metG"
                     /locus_tag="VFR82_05760"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU4501139.1"
                     /translation="MRDQHTFYITTPIYYVNDVPHIGHAYTTIAADVLARYWRLRGRE
                     VFFLTGLDEHGQKVQQAAAKAGVDPQVHCDNLAPHFQTLWKRLNISNDAFIRTTDPQH
                     KRLVQQYLEQLYSKQLIYQDSYTGWYCAFDERFWTEKDVADGLCPDCKRPVERLSERN
                     YFFKMGQYQDRLIEYIKTHPNFIRPETRRNEVLGFLETQKLGDLSISRPKSRLSWGIE
                     LPFDKDYVTYVWFDALVNYLSALEYKPASPSVDRYWPASVHLVGKDILTTHAVYWSAM
                     LMALGLPLPEMIFAHGWWTVDGEKMSKSRGNVVDPNGMVDRFGTDAFRYFLLREVPFG
                     QDGDFSEHAMVRRINSDLANGIGNLLSRTLTMIERNCNGTIPHVPQEYLETNKEKYRL
                     LLHAKEQLLILGKSLDGFFSDLEFNALLLHITSRATDADIFIDEHEPWQLAKDPNKRT
                     ELHAVLYTAAECLRILSLYIYPVMPYTAKNITTQLGLDINFNSPLLSEDIKWGQLQGG
                     TKIQKASALFPRIEFNPQGAKLVSDASIPTSQTTVAPAPTAAGTQAPTTVAQAQITID
                     EFQKIQLKTAKVLSAERVPKSEKLLKLQVSIGSEQRQIVAGIGKKYEPESLVGKTIIV
                     VANLKPAKLMGIESQGMVLAAGDSEVRGLATILEEVDPGTKVK"
BASE COUNT         3323 a         4225 c         4621 g         3443 t
ORIGIN      
        1 ccggcgacgg atttgcagta ttgccgcagg gatatcgttg aggctcatgg cttcaccaaa
       61 gtgctgcatg tggtcgcagg cgggcaggag cgtcaggact ccgtgcgcca ggctctggcg
      121 gttgtggatg agcgggtgga catcgtcgtc attcatgacg ccgtacggcc ctgtgtgacg
      181 gaattgatgg tgaagcaggt cgtggaggca gctaaggccg agggcgccgc aatcattgcg
      241 ctgcccatgc gggatacggt gaaacaggtt ggaccgggtg ggctcatcga gcggacgatt
      301 gatcggaaac cgctgtggct ggcccagacg ccgcaggctt ttcgtcggga ctggctgcag
      361 gacgcgcatc gcaaagcgca cgcggaagga cttgctgcca cggatgatgc ctatctcctt
      421 gaatggatgg gccatccagt cagggtcctc gagggcagtg gggaaaacat caaagttacc
      481 agacccgaag acatgattct cggagaggcg attttgtctt ccagggcgac gcacggtgcg
      541 taggcccgat caatcgtaaa agaagggaga cacatgcgga ttggctatgg atatgacgtg
      601 cacccgcttg ggccgggacg caaactgatt ttgggcggca ttgacatccc gcacagcaag
      661 ggtctgctcg ggcataccga ttccgacgtt cttgtccatg ccgtgtgtga cgcgctgctt
      721 ggggccatgg gcgagggaga ccttggacgg cattatccga gttccgactc caagtacaaa
      781 ggcatgtcga gtctgaagtt actggaagac gtcatgacca agcttaaggt gaagggctac
      841 cgagtgggca acatcgatac ggtcatcgtg gcgcaggccc cgcgtctggg accccacttg
      901 gcggctatgc aaaaaaaaat ggcggaggcg gcgggcatcg atcccaatca ggtcaacgtg
      961 aaagtaaaga gtggagaggg ccttgatgcg gtcggcaagg aagaaggcat gatcgcccat
     1021 gccgtctgtt tgattgagcc agcgtgacca tcgtaaaata gcgagcatgt tcaaggcgat
     1081 tcggcgagac ctcaaagccg tgttcgatcg ggatccggcg gcgaccagca gcttggaagt
     1141 catcttcact tatgcgggct tccatgcgtt gatcgcctac cgtttctctc attggttgaa
     1201 atcctacggc gtgccgcttg tgccgcgagt catttcgcag gtcgcccgct ggctgaccgg
     1261 gatcgagatc catccttcag ctcgcatcgg caccgggttc tttatcgacc atggcatggg
     1321 ggtcgtgatt ggagaaacgg ccgaaattgg agactttgtg acgctgttcc agggcgtgac
     1381 gctgggcggg acgggaaaag aacgaggcaa gcgtcacccg acccttggca accatgttgt
     1441 cgtgggggcg ggcgcgaaga ttctgggagg tatcacgatc ggcgacaacg tcaagatcgg
     1501 ggccaattcc gtcgtgctta agaacgtcgc ggccaactcc acagttatcg gtgttccggc
     1561 tcgcgtgatc aagacgcaag gcgagcggct tcctgacgcc acgatggacc acattaacct
     1621 tcccgatccc atcagcgacc ggtttctggc gctcgagcag gaactgatcg aactccgcaa
     1681 gaaactcgaa aaccgcgacg actctccacc ccgctagcgc accccacagt cgcccgccgg
     1741 atagtccgat tccctactcg tccaccttca aagtgtcctc ttggtcaagg cgttctgcct
     1801 gagggcaccg tttgccgctt atcatgtggt agggggattg gccggtacag aatcaagctg
     1861 gggctcgtag tcgacgcgca cgaggaaaag cccctggggg ggagccgttc tgcctgcggc
     1921 cgtgcgattc ttcgcggcca ggatcattct cagactttct ggcgtccgtt tcgcgtggcc
     1981 gatctcgaca agagtgccca caatcgcgcg taccatgtgt ttgagaaaac ggtctgcgta
     2041 ggcgtgaatc gtgagctgat cttcttgccg tgtcagtgac agctgccgaa ggtggcagac
     2101 cggatcttcg ttgtccgtca aagtgccttc gaaagaagaa aaatcgtgcg agcccaccag
     2161 atgaatggca gcctgttcca tggcggtatg gtcgaggggc ttgtgaacat gccagacata
     2221 ggcccgatcc agggccggcc gttggggccg gttcagaatc cggtattcgt agagttttcc
     2281 acgggcggcg tagcgcgcgt gaaaatcgtt cgggactaga tccgctgacc ggacggcaat
     2341 atcctcgggg agcctcgcgt tgaagcctcg catccattca cgtggcgtcc agtcccgttc
     2401 gatacggaag ctcgccactt gaccgatggc atggacgccg gaatccgtgc gtcctgctcc
     2461 aatgaccgcg acggtctctt gcgtcaattg aaacagcgcc gtctccacgg cttcctggat
     2521 cgttggctga tccggttgac gctgccagcc ggcataccgt gaaccgtcgt attcaaggat
     2581 tagcctaacg gtcggcatgg cggaccacca gggccatgcg gtgcatcagc gtgccggtgt
     2641 tttggaaggg gccagaaagg cggtcacacc ctcatgcagg gattccgcaa tgcgcgttgt
     2701 gaagccggcg gtcctcaaga ggtcctcttc agcggcgttg gagatgtagg cgatttccgc
     2761 caaaatgctc ggcatactgg tgaagcggag cacatagaat ggtgcggttt tcacaccatg
     2821 atccacgagc gcgtaatcac cgttcaagcg agtgatcatc gcttctttgg tgttccaggc
     2881 cagctcaaga gaggcttcaa tttttttgct cgtgaggaga tcggccacga gatattccca
     2941 tcccactcca gtgctgctga gaggagtgcc gttttcacga gcagccaact cgagagcacg
     3001 ctggtccttc gcttcgccga aatgatagat ctcgatacca cggaccgatc gttgcggatg
     3061 cgagttgacg tggattgaca ggaacaggtc ggcttcgttg ccgttggcaa atcgggcacg
     3121 gtcttccaaa tcaacgaaca cgtcccgctc ccgggtcatc agcacacgaa cattggggtc
     3181 cttccgcaac aggtcgcgta gtttcaatcc caccttgagc gtgatgtcct tttcttcggt
     3241 accgcgctgt ccatgcgcgc cgggatctcg tccgccgtgt ccgggatcaa tcacaatcgt
     3301 cttataggac ttggcgcgcg gtgcaaccgg ttgcggtgga ggggcagaaa acatagatga
     3361 gtctgtgggg tcctgaacgg atccggacgg tggcagccca gagggaatga cgtcaaccac
     3421 cagccgagga gggttggtca atgataacag actataggtt tcgaacgagc cgctcggcag
     3481 ggcgaccgtg acggagtcac cgacggtttg tttgacgaca aagcgtccct ctaaaactcc
     3541 ttgcgcaatt ttcgcctggg ctgatttggt caatgcggcg tgagggatct cgacaaccac
     3601 tccgtcctct tgagcgggac ggtgtttttt tacctgcgtt ttgcggtcaa gatcgagtac
     3661 gagccggaga gaatccggca aaaatgccgt tctcaaattc tgaattttcg cgagcgggat
     3721 cgtgttgggg gagaggctga cgttggagtt ccgtgatcgc ttggagttcc tctgatgatc
     3781 gcgcctttgg tcctgcggtg ctgcgtgtgc tgctccaagc caggcaccgt ttgccaggaa
     3841 gtcctccgtg ctgagaagtc ccagcactag gatcaaggca gctacgcgag agacagttgc
     3901 gggcatgggg tttccacaga aggctgcggg aagatatatg tatctctcag atgttctggt
     3961 agttgtcaag gggagcgacg tttctcgagg cggactgcaa atgattgaca cctccttgga
     4021 gcgcgcgtag ggtctgctgg tcatgcatgt catcatcgat ggctataatc tcttggctgt
     4081 tgtccggcca ctgggtggag caaatctgta ctcagacacg gcgagagagg ctctgttgcg
     4141 attattgggt ggatatcggc atcgaaaagg ccatgcattg acggtcgtct ttgacggctg
     4201 gcaacgggga cagccgatcg aaaacaggga gcaccgtgca ggagtccacg tcatttactc
     4261 caagcgaggc gagcgggccg accaggtcat ccagcggttg gcacgagaat atggcgccca
     4321 atgcgcggtg gtcagctccg accacgaaat cgtgaattcc gcacaggcgc acggcgcgct
     4381 ggtcatcagc gcgcgagcat ttgcagacaa attacataca caaggctcat ccgagacctc
     4441 ggtcccgtac aaggagttgg atcccggtga agacccggca gagcgacgaa ggccggagaa
     4501 gaaaggtaat gcccgaaaac ttccgaagag tcttcgccga cgcagccgac aactcaaggg
     4561 attttgaaga gtcgttttgc attggcgctg gtggtgcgct caatgtcctc gtacgataga
     4621 tctggacgga tcgcgaccag ttgtttggcg acgtggggga cgaacgccgg ttcatttcgc
     4681 ttccctcgat gcggaacggg tgtgagatag ggacagtcag tttcaatcaa cactctatcc
     4741 aaaggcgtct gcttggcgat atcccgcagc atcgcggcat tttggaaagt caaaatcccg
     4801 gaaaaggaaa gataaaaccc cagatcgagt gcatccttgg ccagccaggc atcccctgag
     4861 aagcagtgaa acaccccgcc gacttccgcg gctttctcct cttttaaaat cgtgatggtg
     4921 tcctcttgtg cctctctcgt atgaatgatc acgggcaatc gcaactcgcg tgcaagttgt
     4981 atctgttcgc gaaaccgctg ccgctggctc tgaggggatg aatggttgta gtgataatcg
     5041 agtccgatct cgccataggc cacgactttc ctgtgttgcg caaggcggcg aaagtcgctg
     5101 taccacgcgt cttcgacgtg tttcacttcg tggggatgca cgccaataga cgcaaagacg
     5161 tccggatgtt gatcggcaag cgccacggcc gaccggctgg tggccagatc gcatccgatc
     5221 gtcacgaatg cttcgatgcc ggcttgccgc gcgcgctcga tcattgcgtc gcggtcgccg
     5281 tcgaaccgag catcgtcgag atgggtatgg gtgtcaatga gcatgcaatc accgagagca
     5341 aggacctata ggagggacgg cgaggagccg gttagagggc tgttgagcaa ctgctctgac
     5401 cacctgaaat cgtgaaggca tgcacacgca acggctactc ccgtgtgtca ggcaggtcgg
     5461 gcgacgatgc tctcggcgag ttcattgagg agtgcggcgg tgtcgtccca acccaggcag
     5521 gcgtcggtaa tggaaacgcc gtgagcgaga gcgacgcctt gtttccacgt ctgttttccg
     5581 gggttgagat tgctttccag catcaagccc atgattgagt ggcgcccgtc acggaactgt
     5641 cttagcactt cccgagcgac cagggcctgg cgcgtatggt ccttgctcga attgtcatgc
     5701 gagcagtcga tcatgatcga tcgtgcaatg ccttcgccgg ccactgcggc ttccgctttc
     5761 gccacatgtt cggcatcgta attggtttta ccaccacccc cgcgcagcac gatgtggcgg
     5821 tctgggttgc ccatggtctt aatgatcgac gtttggccgt ctgcgttgat tccgacaaaa
     5881 tggtgagggg ttctggctga agtcatggcg ttcacggcga cgtgcagact tccttccgtc
     5941 ccgttcttga acccaacagg catggacacg ccgctggcca tttcccggtg cgtctggctc
     6001 tccgtcgtgc gggcaccgat cgcagtccag ctaataaggt cggcgatata ctgtgggcta
     6061 atgggatcga gcagctccgt ggcgcagggc atgcccgatt gattgatctt gaggagagtc
     6121 gtccgggcga gttccatccc agtggcgata tcgcaggtac cgtccaaatg cggatcgttg
     6181 atgagccctt tccagccgac tgtggtccga ggtttttcaa aataggtccg catcacgatg
     6241 aggagccggt cacgaacggc gtccgacaac ggcttcagtt ttaccgcata ttcgaaggct
     6301 gcttctggat catggatcga gcagggacca acgatgacaa ggagccgatc gcgatcttga
     6361 ccgtggagga tccgccggat tgcttcccgt gtttccacga ccagcgcggc gacatcgtcg
     6421 gtgatcggaa gtttcgtctt gatggcccgc ggggagggca acgccttgat ttctatgacg
     6481 tgttggttgt cgagcggtcg attcatggcg gcctttcagt agtcgaagtg tggcagcata
     6541 gcgaaatgtt caagaaaagt ctacatgatc cactctaagt cgttgaaaag atgtagactg
     6601 gttggcaaat cacctgattt tgacagcatt tgcgaccttg tgatacttag cagcatgtct
     6661 ccaggaatgt cctctcccgt tcaggcgctt tctctcagtc ttatcctatt gattcttgtg
     6721 ttcgcgccgt tcgctgcagc acaggacatc catcacgaac tggcggcggc tgatacagat
     6781 gggcacgagc actctgatac cgatctgtgt caatgggtgc agcatcacac gtccggctcg
     6841 gtcgatatcg gtgggccgat ctgctcggct tggcaatcta cgggtcagta tgacgttccg
     6901 cctcactcga ttctcttctc gctcgattcg acgacggtcg gcccctcccg cgcaccaccc
     6961 cattcctaac gggcgaatta ttgcgacgtc atagcttggg cggatcatcg cccgcctatg
     7021 accagcatct ggcgtcatcg ctcgtgattg cggaggtggg tatgcgacgg gaaggttcat
     7081 gctattgctg cggtgtgctg ttgatgacag gagccgggtg gctgatctcg agtgaaacgt
     7141 tgctcaacaa cgccgcagcc aaagattcag gacagacaat cgtcggcaca gtgcagaatc
     7201 aagacctacg acgagtagat caggccacgg ttcaggtgcg tgatcaagaa gggaatatgg
     7261 tggcccaaac ggtcaccaac caggggggag aatttcgagt cgtggtacct cagttaggca
     7321 cctattcgat cagtgcggtc cgggataccg accggagtga gtatgtcgtc gtgaagatag
     7381 gcgccgagca gccagcgcct gtatcgctta cccttacggc gaccagagag attgctctgg
     7441 agattgtatc accacttcct gccattcagt acaaagcctc gagcgagaca tatcagctga
     7501 gccgaaaaga aatcgagacc ttgccgagag ggaacaacaa tgacgtgcac gacgtgttgc
     7561 tcaccgtgcc aagcgtcgtc tatgggggcc tcaaacaaac acacattcga caagaccatg
     7621 ccaatcagca ataccgcatc gacggaatcc ctatcccgga tacggtcagc ggcgcctttg
     7681 cagatattgt ccccccccgt gcatgggaac gagcagacat cattctggga ggcatggaag
     7741 cgcaatatgg aaacaagacg gcactcgtgg tggacatcac aagcaaaagc gggacgactc
     7801 ccggcttcgg ctccgcccag gtattcggcg gatcgaatca gacggtaaat ccatcctttg
     7861 aatacggtgg tgccatcggg gaaaaagttc ggttttatgt gttgaacagt tacgtcacga
     7921 cgagccgcgg catcgagccg ccgacgctag gcaagaccaa ccatcacaat cagagcgaaa
     7981 agaacaatac ctacctgcgc ggcgactatc agcacgacaa ccgcaacaac ttcagttgga
     8041 tctttctcaa ttctgtggcg aagcaccaga tcccgacggt tcccgatctg gcccctaatc
     8101 cagacgtgct cgcgttgttg ccagggttca ccccaactgc ctcacagtct gtcgatcagt
     8161 ttcaaaagga agacagccaa tattcgcagc ttgtgtggag acatgacgtc aacgccagca
     8221 acttgttcgg gctgggcctg tattttcgac ggggggttgc ggattttcgg acggatccat
     8281 cgaatgcgtt ggcctatact gaggacgtca gttcggctca gacagcgaat cagcggcgag
     8341 tcgcctacag cgggggtgtg cgtttcgatt acacctggat ccccgacagc cggcatctgg
     8401 tcaagactgg cttccagctg gactacacca gggccaacaa ccagtcgcaa atttttgcct
     8461 tcgatacgag cagcggcgcg ccggccggac cggtattatc cctgaatgcc tcgaatcaaa
     8521 acatccaaac tagacaggaa ttgtgggtgc aggaccaatg gagtccgacg gaagattgga
     8581 ccttcaatct cggggtcagg ggtgacgcca tccaggggtt ctataatgag ggccagatca
     8641 gtccacgagt cggtgtgacg tacaagtaca atcgatcgaa tgtctatcac gcgtactatg
     8701 gcaggctgtt tacgccaccg aacgttgagc aggtggcgtt cgcgcaactg aatactcaaa
     8761 atacgacggc cgcgccggac aatccgaccg gttttcgccc tcgcgccgaa cgctcccatt
     8821 actttgaggt cgggagttat catgcattgc accgaaacgc gacgctcgaa ttaaccggtt
     8881 attacaagct tgcccactat ttgtccgatg cgggccaatt cggcagcaca cccctcttga
     8941 acttcttcgc cttcgaacgg ggatggcaac gcggtatcga cggggcgtta aaagtgcaat
     9001 tcacggagaa ctttaccggg cgcgggaacg tcgcgtgggg gcaatgcaaa gggtacgggt
     9061 tgcagtccgg tcagtacctc ctcgatcagg tggagatcaa tgacatcaac tcgaccggcg
     9121 gcgtcttctg cgatcattcg caatttgtca cgagctcggc ggtgttgtcc tatcgattat
     9181 gggaacgcac gatgttcacc ggccaaatgc tctacgggtc cggcttgcgg acggcggagc
     9241 ccggagcaaa aacgaactcc tcccacgaga attcctatac cgtttacaat gcctcgatca
     9301 cccataccat tttgttgcca tggcatggtc aaaaaatgct ggtgggcttt gacgtaatca
     9361 acctgctcga tcagcactat tttatcaacc ggggagaagg cagcatcggt ctcgggattg
     9421 cgcatgccgg gatgccccga tcctatttct ttcgcgcaca atggttcttt tagattatga
     9481 tgcggcccgc tgggaggtcg tttgagcagc ctcatgccaa atgctcttgg ccatatgtca
     9541 attgaacaag gtgcccggtg acacacactt caacgtcttt tgtcgctctc atgctatgcg
     9601 tcatctcgct tgcagtcggg tcgttgccga tttgtaccta tgccgcggcc ggtgatgagg
     9661 cgacacatga gagcgaccat caggaggacg tgttggagct gccggaggtc cacgtccacg
     9721 ggctctcgct caacaaggat cagcagatcg gaccagttcc gaaatccaca ccttggcccg
     9781 gcatccctcc ggccctcgag gggagcgtca tcgatgattg gatgaaagcc cggatgctgg
     9841 tgtcaaaaga ggccaaggtc acggtcgtga tccttgaacc ttgccgacat cgggagctga
     9901 ccgctgcggg catcgctgct ctcgcaaaat ggacatttga gccacagatg caaggcgatg
     9961 tccctatcga cggggagttg accgtgcgcc ttcacttcca tacgaagtag cggacgactg
    10021 ccgttgacga attctcaatc gcactatatg gtcagacgat gagttgggac gtcttcctca
    10081 cacgatcgat caatgggtta gcaggaaaac atgcatggac tgattggtta ttcctgactt
    10141 tgtcgaaccc cgacatgctc tggctgccgg gcatttttct cggcacctat tggctgtggc
    10201 ggtctccccg ggaagcattg atcggcgggc cgatcctcgg tgtctccatc ggaatattgg
    10261 atttcatcgg cgctcaacta aagtatctgg ccgctcgtcc gcgtccttgt gtcagcatgg
    10321 ccgacctcat ccagctgcag gcttgcggaa aaacgttcag tttcccgtcc aaccacgcgg
    10381 tcaataccgc cacggccgcg gcgtttttcc aggtgctcta cccacggtcg ggctggatca
    10441 gttggcccat cgtaggactg atcggtatgg gccgggtata tatcggcgcg cactatgtga
    10501 gtgatgtgat cggcggatgg atgatcgggg gactttgcgg tgccggcatc gcctggctgc
    10561 tgttgcaagg gccgcgattt cgtcaacggg tattgtcgcc gatcgtctcg acgcaagctg
    10621 atagctctgt gcaataacga tccacgtcat agcctcttcc gatcaggaca atcgccttct
    10681 ctggttcgtc cagcagcatg accggtaaaa tcgtcggctc gccgggcggc gcgtattgga
    10741 attcctgcaa ctcgggttcc tgtgtgaagc ggagaaagcc tttggcccgt tcgatctcct
    10801 tgggcaatgt tttggcccac gacaagaagc gatcccggtt caaggtcccg gataagcgaa
    10861 ccgtagtcgc catcggatgg tagcccgcgc gcaggtgcga agtgggtcgc gatctgccga
    10921 actcgacgtt ggtgggttcc gcgctgtgtg agcggtcatg cccactcaac aaggcagccg
    10981 catcaattcg tgcgtgggtg gtttcccaca ctcgagcatg agcgttgcgg gcaacaatcg
    11041 atgccttgaa ctgttcccag tggccgggca cgtacagatc ccgcttgttc aagatcaatt
    11101 catcggcgta gcgaatcgcc tgttcggtca caaatgtgct cgaagggctc tggtcgttgg
    11161 cgatcggctg aagcagcgca atgatccgtt caagcttggt cagtctggcc gtgtagagat
    11221 ccgtcacccc gtcgaccact tcggccggat ccgcgaggcc ggaacattcg agcacaatca
    11281 actcggatct atactctctg acgagttgcg cgattcccca ggacagatcc tcctttgtgt
    11341 cgcagcagac gcatcctccc gcaagattca ttacctgctc ggcaatggta ccgacgcgcg
    11401 gtccgtcgat gctgatttcg ccggcctcat tcatgagcac gccggcccga atgcccaacc
    11461 gtttccaatg ttcgagcagc cgcatcagga gcgtcgtttt tcctgcacca agcgatccgc
    11521 agaggatgta gaaaggcacg gcaggctgag tcatggtcac gggttaggga tgggcgtgga
    11581 gacgatcgtc ctggcggctg gccaatgcca tgaggtcgca atggaccgca tccttgtgag
    11641 cgcaatgggt ttcgagctga atggtcatat gcgtaatgcc gaaatccgaa gtgatccggc
    11701 aacggatgtc acgcaacaac tcgttggtga ccgcagtgtc atccgcatcg atcagcacat
    11761 ggcaggtcag catgaccagt cggggttcga ttgcccacac atgcaggtcg tggacattgt
    11821 cgacaccggg aatcgcttcg atggtggcgg cgatatgaga ggcgctgacg ccaggcggcg
    11881 ttgactccag cagcacctcc agggattcgc gaaaaatcgg caaggcgccg cggataatcg
    11941 ctccaaccag aaggatgctg atcagcggat cgagaaccgt ccaaccggtc gcaacaatgg
    12001 ccgccccgct gaggattact ccgagtgaga cccaggcgtc ggcaagcatg tgccaaaacg
    12061 cgctgcgaat attgagatca tcctttgcgc cgtgctgaag caagagcgcg atcccgagat
    12121 tggcggcaaa gctaatagcg gcgattgcca tgacgccgct gccgtccacg gagacaggat
    12181 ggatgagccg ttgcacgccc acgattgtga tgcccaaagc ggtcaacaag agaacgaacg
    12241 agcttagaaa tgcggtcacg atgcccgctc gatggtagcc gaatgttcgc gcttccgtgg
    12301 ccggtcttgc cgtcaagata tgcgcataga gggcggcgaa cagcgcgccc tgatcgatga
    12361 ggttatgacc ggcgtcgccg attaatccga tgctgttaat gaggatccct ccgacgaact
    12421 cggcgacgat gacgatcgca tttaagccca aggcaagatg taagcgcgat cgcaaatgcg
    12481 tataggcggc gcgtcctgtc ataagacata gtctcgcatg tgcacggtga accagcaagt
    12541 catccgaaag acggcgcttt tctggtcaag gattcgtcga cgatcggagc ggtttttgta
    12601 tcaggcaggc ggcggttccc gcccctgagt ttcccgttgc cagatcggtg gccaccactc
    12661 gtaagaggat gccgtccggc gcctcatgcg ggttcggcac aaaaaatggt gcctcgaatg
    12721 tccctgtttc attggagaaa aatagttgta ggcgttcgac gatgcgatcg ccgatcagta
    12781 actctccata gatccggatc tttcgagaat cccaatctcc atggggtctg accaacgtac
    12841 cggataacgt tttcactgat gcacgtaagg tgacgtcgtc cgttgccatg agcgcttggc
    12901 cctgtttggg atgctcgatc tgcacgatgt agccatagag atgcagcacg atgccgtcgt
    12961 tcgtgagatc ttgtcctgga atgagcagca cggtctgtga tgcgtgctgc atcgaagccg
    13021 gatgtgcgag aggtccctcg gcggtgattt ccaccaaggt gggcctccga agctccaccg
    13081 tcgtggtgaa ggcggccgca gggcgggcac tgtacaccgg ctcttccatg aggtgggggg
    13141 tcctcatgat ttgattctga tctcctgcct cgccttgctg tatgccggtg gcaagaacct
    13201 gcttggtctc gacgtccgtg atggtgactc tcgcgccccc gacttcacga ccgatgacca
    13261 tcgcgccatg agcgacgacc cgaacgagaa tcgtcgtggg ctttggagca tttaatcggg
    13321 gatcgggagg aggctcgcct tccgctggga tcgcccagca gggcggaggg atgaggagcg
    13381 ttaacaccca tgcacaggca agaagacgga gtcgcgtcat ttgactttgg tgccgggatc
    13441 cacttcttca aggatagtag ccagccctcg gacttcactg tcaccggccg ccagcaccat
    13501 cccctgtgat tcgatgccca tgagtttagc cggtttcaga ttggcgacaa cgatgatggt
    13561 cttgccgact agcgactccg gctcgtactt cttcccgatg ccggccacga tctgtcgttg
    13621 ctcgcttccg attgagacct ggagcttgag cagtttctcg gacttgggca cccgttctgc
    13681 gctcagtact ttggcggtct tcagctggat tttttgaaat tcatcgatgg tgatttgcgc
    13741 ttgcgcgacc gtggttggcg cttgggtgcc ggcagcggtc ggtgcgggtg cgacggtggt
    13801 ctgtgacgtt gggattgatg cgtcgctcac gagtttagct ccttgtgggt tgaattctat
    13861 gcgcggaaaa agagcgctgg ccttctggat ctttgttcca ccctgtagtt gaccccattt
    13921 gatatcttcg ctcaacagcg gcgaattgaa atttatatca agtcctaact gtgtagtaat
    13981 gtttttggcg gtatacggca tgacgggata aatgtacaaa ctcaaaatac ggaggcattc
    14041 tgctgcagta tataagaccg catgcagttc agtccgctta ttcgggtctt ttgcgagctg
    14101 ccaaggctca tgttcatcga tgaatatatc tgcgtcggta gccctggacg taatatgaag
    14161 aagcagagca ttgaattcca gatcggagaa gaaaccgtcg agagattttc ctaaaataag
    14221 tagctgttcc tttgcgtgaa ggaggagacg gtacttctct ttgtttgtct caaggtactc
    14281 ctgaggcaca tgagggatcg tgccattaca gtttcgttcg atcatcgtca atgtccggct
    14341 caagaggttg ccgattccgt tggccaaatc gctgttgatt cgtctcacca tagcgtgctc
    14401 ggaaaagtcg ccatcctgtc cgaacggcac ttcccgcaag agaaagtagc gaaaggcatc
    14461 cgtcccaaag cgatcaacca tcccattggg gtcaacgaca ttgccgcggc ttttggacat
    14521 tttctcgcca tcgacggtcc accatccatg agcaaagatc atctccggta acggcaatcc
    14581 caacgccatc agcatcgcgg accagtacac cgcatgtgtg gtcagaatat ccttcccaac
    14641 caaatgcacg gaggcgggcc agtaacggtc gaccgaaggg gatgccggtt tatactccaa
    14701 cgctgaaaga tagttgacta acgcatcgaa ccagacatag gtcacgtagt ccttatcaaa
    14761 gggcagttct atgccccagg agagccgtga ctttggccgt gaaatagaca ggtccccgag
    14821 tttctgggtc tcgagaaatc cgaggacttc gttgcgacgc gtttcagggc ggatgaaatt
    14881 cgggtgggtc ttgatgtact cgatcaggcg gtcctgatat tggcccattt tgaaaaagta
    14941 gtttcgctcg ctcagccgct cgaccgggcg tttgcagtcc ggacacagtc cgtcggctac
    15001 gtccttttcg gtccaaaacc gttcatcgaa tgcgcaatac caccccgtgt aagaatcctg
    15061 gtagatcagc tgcttagagt atagctgttc aagatattgt tgaacgaggc gtttatgctg
    15121 gggatccgtt gtccggatga atgcgtcatt ggaaatgttg agtcgcttcc acagcgtctg
    15181 aaaatggggc gccaagttgt cgcagtggac ttgcggatcc acgccggcct tggcggcggc
    15241 ttgctgaacc ttctggccgt gttcgtctag acctgtcaga aaaaagacct cacgacctcg
    15301 taaccgccaa tagcgcgcca gcacatccgc ggcgatcgtg gtataggcat ggccgatgtg
    15361 cgggacgtcg ttgacgtaat agataggcgt ggtgatgtaa aacgtatgct gatcgcgcat
    15421 ggttaggttt caaaccttaa cagtcgaaga ggacgaaagc caacgtctgc actggccgct
    15481 ctcacaagac tggctgggga actggcgtca tgatcgcatc gcgcaggcga agcagaatgt
    15541 tctcaagcgc catctgcaaa ttcagatgcc tggtcgcctg tcgtagggtg tgatcgatgt
    15601 cgctcagcac gg
//
LOCUS       DASTQY010000177        10547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1042500, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000177 DASTQY010000000
VERSION     DASTQY010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10547
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042500"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(802..1551)
                     /locus_tag="VFR82_05765"
     CDS             complement(802..1551)
                     /locus_tag="VFR82_05765"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4501140.1"
                     /translation="MRLNVASCLVAGGIVTALIGCGLLETSKKSTTATTHAQRHQAAA
                     VIGNSVASQVEYSPTDAVKDTVADLLSILGNEALRQPNRSLERRHSIEDIIRHRVSYE
                     EAAQRALGLQWERLTDSERQEYVSLFMQLLRDTLANNIDQYYDEEMLYLSERRHGRFA
                     EVRTNLVGSKVNTSLYFRLGSQSGQWRIYDVVIDGTSLIRSYKAQFTRIIRDDSYAGL
                     IEKMKQRNLLAKVFERTAPAVALSSMHTAPQ"
     gene            complement(1657..2160)
                     /locus_tag="VFR82_05770"
     CDS             complement(1657..2160)
                     /locus_tag="VFR82_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501141.1"
                     /translation="MPNLDLTSILFWASAAAGLAVMYYSFVLAREWFRQWHPSGKGIF
                     RRNTVAETSLNAHIAGQWTEGRLRLDSVLTLIQSLADLLEKEVKALRRLDHSDPQYEG
                     EVRQMVEPTLRCFTFDKHLRDECRVLIPLLRFCAQDLAQSQGLDMTQKLIVLDEIQAA
                     IELADLH"
     gene            complement(2835..3485)
                     /locus_tag="VFR82_05775"
     CDS             complement(2835..3485)
                     /locus_tag="VFR82_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU4501142.1"
                     /translation="MARARTTFDPQLFLTKVGKGKTTLESPKKHTIFSQGDAAEAVFY
                     VQAGKVKLTVVSQQGKEAVIAILERGAFFGESCLAGQAIRTATATAVEDSSIVRIDKE
                     AMLLVLHEEPTFAELFMAYLLAHTIRIEEDLVDHLFNSSEKRLARVLLLLAHFGKEGK
                     PETVIAKISQETLAEMIGTTRSRVSFFMNKFRKLGFIEYNGELHVHSSLLNVILHD"
     gene            complement(3620..3835)
                     /locus_tag="VFR82_05780"
     CDS             complement(3620..3835)
                     /locus_tag="VFR82_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501143.1"
                     /translation="MMKTTPQTYVLTLNSPSRKAVRLWTGKSIHTLRHCSVTQLNHAL
                     VELQQYVEDLQAPVRTPGASYSLNRSR"
     gene            complement(3907..4275)
                     /locus_tag="VFR82_05785"
     CDS             complement(3907..4275)
                     /locus_tag="VFR82_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501144.1"
                     /translation="MNVQPTVQSHIDMTDTLSRLYLFLAQSLDRCVSEAARISYPEPQ
                     FQATLASTKAAVLDMLSVNQVVKAKVAQECERIETLVMACLKDGAAKADALDELKAER
                     AVLKHKTLALSDLLAVFRAA"
     gene            complement(4318..4518)
                     /locus_tag="VFR82_05790"
     CDS             complement(4318..4518)
                     /locus_tag="VFR82_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501145.1"
                     /translation="MTPQEQITKMTTAIRQAIKVMGDRFGSTEQDVATAIHELKISMQ
                     KETEPMAIAGDSTTAKQPGSAG"
     gene            complement(4756..5688)
                     /locus_tag="VFR82_05795"
     CDS             complement(4756..5688)
                     /locus_tag="VFR82_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501146.1"
                     /translation="MQRRFEFLDVIVVVGLCATIVASGVLLMAANGMQIGSVGTGTIG
                     QPTDNLTGMDLLQPVLGQAIVDHVLLERRHAKDAAAAITQLGGLTFERNQWQHFPYSY
                     LDPVTTGASWAESEHTARVQTVMGRSIVNFTRRGVRSGLWSSVERAAHDNPKMIGVTK
                     AMGQNMDRLFLANWQPNIGRGIVAATQENETRSTLRQEQLGTAILEMATVQEVYEPVR
                     AAIQEQLGSATVVATMPGSQQRGVTSDRLTADRVAALSAQDALPRISTTTTFLVASLI
                     LISLFTTGLFVLSSRRAEVPQNGLVRALVPPKLL"
     gene            complement(5804..6142)
                     /locus_tag="VFR82_05800"
     CDS             complement(5804..6142)
                     /locus_tag="VFR82_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501147.1"
                     /translation="MRLFTMTIAGLMFVGMTGLALANPSMLPTHPGYPSGGEFANDTG
                     QQNLTHSQSLMEAAKSGDTNMTPTFMDQNTVNQNNARISERDGAGQSPVVGANAKTEP
                     SGKETERKTK"
     gene            complement(6588..7484)
                     /locus_tag="VFR82_05805"
     CDS             complement(6588..7484)
                     /locus_tag="VFR82_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU4501148.1"
                     /translation="MNIVERRETDVERCRRPGHAGMAASLLVALAFLGWSADEALSAA
                     ITIAGNGPELRMVERLTSAFEKKYFGAVAEIRWDPSFQPSHMVKTGEADVAVVGQTDP
                     DLLVTPIAWDGIAVLVDFTNPVREITSVQLAAMFSGTATRWSDVGGNDTRIELINSPS
                     NQPIRGTFETVLDIVGKSPPTAYVSRSDQRAISRLAGNGSAVTYASLGVALDAVTYGV
                     GVALLSVDQVEPAKQTVRDGRYRLRRPVLLLRKDEANSTATSFVEFAVSKEAKDIIDE
                     MYVSYTGPDNDPHAVRSSMREK"
     gene            complement(7710..8117)
                     /locus_tag="VFR82_05810"
     CDS             complement(7710..8117)
                     /locus_tag="VFR82_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501149.1"
                     /translation="MPKFLHLLVSAMVIAVPAAGLAQFSDKGDRVEVKKGTRLCSSIA
                     VDGVRGTLNQRPIFWRVTSPIRANGTQTVSALVHETVYNRQSGDTTTVENPYDEDFAT
                     FFKRAPEHTGMLQMISPQDGTVQAIVEICGKGK"
     gene            complement(8167..8664)
                     /locus_tag="VFR82_05815"
     CDS             complement(8167..8664)
                     /locus_tag="VFR82_05815"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEU4501150.1"
                     /translation="MTTLLRLRSGWTNTNPTTDAKTIGAMETLDGLGYITTGFSFLLI
                     AMVVFAHSWYVFLLSVRSQAVPSVLALVHDLLLVIILLELFRTIINFLKTKVITLEPF
                     LYICVIASTRRILTTGAQIAYMENLSDQVFHRYLLDLGANVLVVLALVVAVYLVRRAV
                     PAAVS"
     gene            complement(8823..9116)
                     /locus_tag="VFR82_05820"
     CDS             complement(8823..9116)
                     /locus_tag="VFR82_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501151.1"
                     /translation="MKVCLTMKRLVLLSSVTFMLGATVSSVDAEDRLVEGAMILAPAE
                     GRSSMGNISEDTLEACLARISERVTPEQRMLAQQVCHADEATRQLEPLGVKGD"
     gene            complement(9450..9695)
                     /locus_tag="VFR82_05825"
     CDS             complement(9450..9695)
                     /locus_tag="VFR82_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501152.1"
                     /translation="MNQEQFGQFWLQLRDPLKAKWEKITTEDLDEIQGDLVKFGSVLQ
                     RRYGEMHKEEVTIWANRRYSHWTGNYAGYKDPEHPAK"
     gene            complement(9821..10345)
                     /locus_tag="VFR82_05830"
     CDS             complement(9821..10345)
                     /locus_tag="VFR82_05830"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEU4501153.1"
                     /translation="MARYVFVAVLVATFLTTASLQAARKLDAKETAFLQKAAQGQLAD
                     IALGTLALKKASHKDVKEFGAEISEDHQYASQEVKELSASEGIYLPVQLDSEHKKQQQ
                     RLSHLSGKEFDEAFVAYMLENHRKDLKEFEKNAAMLRNEKVKQWAEGILPVLTVHLKK
                     AERVAEALGFGQAE"
BASE COUNT         2339 a         2979 c         2673 g         2556 t
ORIGIN      
        1 atgtttcctc gatacacaca acctagtctg acgcgatcat caggctatga cgagggagtg
       61 gttgaccggc gaatagaagc gggagcagga gtacagagcc taacagggaa tgtggaagat
      121 agatagaagg ggctccttag acccccgaca tagcttcaaa atcacagact tatcatcaat
      181 cattgtgggt caccctgaag gctattgatt ctccgcttct cgcatccccg taccatttga
      241 tgtatgtgat ggctcgcctg ctttccagac cagtacaccg tgtcgataga cttcgaccac
      301 cgaggctgag tatcgccgaa cgattacggt gatatgctcg ttgattcgca tctccatatc
      361 ctggcttaac actcttgcct ttcacggccc caggggaccc caggcgctga actgcgtagc
      421 tttatgatca cctgcttcaa catttgctgc atctgttcat gctcttggtc agagagatta
      481 tcgactttta agaggacttc gctgaggcgg atatattcca gaacattatg gcggtactcc
      541 aacttactcc gctgtcgtgg atgtagcttt gaaagttcca ctgcataata ttcaattata
      601 ccctcttcgt cttcggataa cgctgaacgc aagagagtgg gggacaatag agagttgcag
      661 cattgaagat aatcttgaat ctctttgcgg atggacatgt ccaggtccgc catagaccac
      721 cgcctttctc acacaaagac agcggttgag ccgtcgagga gttatcgagc agttgtgact
      781 tcctgccaaa agaggtgcta gttactgagg agcagtgtgc atcgacgaca gggcgacggc
      841 aggtgcggtc ctctcaaaca ccttagcgag aagattcctc tgcttcattt tttctataag
      901 gcccgcgtag gaatcatcgc ggataatccg agtaaattgc gccttgtagc ttctgatcaa
      961 gctggtgcca tcgataacaa cgtcgtatat tcgccactgg ccggactggc tccctaaccg
     1021 gaaatacagt gaagtgttca cttttgatcc gacgaggttg gttctcacct ctgcaaagcg
     1081 accatggcgc cgttcagaga gatacagcat ctcctcgtcg taatactggt cgatgttatt
     1141 tgccaacgtg tcacgtaata actgcataaa aaggctcaca tactcttgcc gctctgaatc
     1201 agtcagtcgc tcccattgaa gtcccaaagc acgttgcgcg gcctcttcat agctgacgcg
     1261 atgtctgatg atgtcttcga tggagtgacg tcgctccaga gatcggttgg gctgtctcag
     1321 cgcttcattt ccaaggatgg acagcaaatc tgcaaccgta tccttgaccg catccgtggg
     1381 ggaatactcg acttgactcg cgacgctgtt cccgatgact gccgctgcct ggtgtcgttg
     1441 agcatgagtt gtagcagtcg tgctcttttt tgaagtctct agcaagccgc atccaatcag
     1501 cgctgtgacg atcccgcctg ccacgagaca tgaagcaaca ttcagtcgca tgaaccgatt
     1561 ctatcaattt acaggaggac ccactggtca aaattgctca catcgacaga aattaatgcg
     1621 atcagggagc cggtgcggcg caccggtctt cgagtgtcag tggagatccg ccaactcgat
     1681 ggcggcttga atctcgtcga ggacgatcaa tttttgcgtc atgtccaaac cctgcgattg
     1741 ggcgagatcc tgggcgcaga atctcagaag cggaattaac acgcggcact catctcgcaa
     1801 atgcttgtcg aaggtgaagc agcgcagggt gggttcgacc atctgacgca cctccccttc
     1861 atactgtgga tctgaatgat cgagtcgccg cagtgctttc acttccttct ctaacaagtc
     1921 cgcaagtgat tggatcagag tcaacacaga gtctaagcga agacggcctt cggtccattg
     1981 accggcaatg tgtgcattga gagaggtttc cgccacggta tttctccgga agatgccttt
     2041 gcccgacgga tgccattgcc tgaaccactc acgtgcgagc acgaaggaat aatacataac
     2101 cgcgaggccg gcagcagcgc tcgcccagaa caaaatggag gtcaggtcta gattcggcat
     2161 gatggcttgt ccgttcccgc tgtaattgat ccggcgcagt cttcgctatg cacaactacc
     2221 tcggctcctc gattcattga cgtcctgagc aggttcgaga ccgtttcgac tcggccaccg
     2281 gagtgggacc catgagaaca gcgagaaaga agatagttcc acaacagtga gagttccatg
     2341 ctatgccttc gagaaggagg aagctggtca caactgctcg ggacactact tgattcagtc
     2401 accgggtgag cctgcgcgcg ctgtatcgta tcctcagctt ccagtgggca tcgtatacca
     2461 accctgcgcg aacacgccgt gatctgtttt gatcagaccg ctccagccag tttttgtatt
     2521 gttcaggctc gctctgtcga gctatccgag aaatccactt ctcaccttaa gcgaaatggc
     2581 gctttgaatt atgtccacat gcgatatctg tcacgccgat gatgttgttg cccaacgttc
     2641 gtcccgcggg acagacgcgc tacagcgact ggtggtacac cagcaatgca ttcaaggtca
     2701 cagatggcat ctggctttcg aggatgcaac ttacccactg ggtggcagag atgtcctggt
     2761 ctgtgactgt ccagaagagt gacgcggcgt tctggaaagg ttccggagtg tgcaaaaacg
     2821 cgacgggcca cgccctagtc gtgcaaaatg acattcagca gggagctgtg cacgtgcagt
     2881 tcgccgttgt attcaatgaa acccagcttg cggaacttgt tcatgaaaaa actcacgcgc
     2941 gagcgtgtgg tgccgatcat ctcggcaagc gtttcctgac tgatcttcgc aataaccgtc
     3001 tcaggcttcc cttctttgcc gaaatgagcc aataagagta atacgcgggc gagccgcttc
     3061 tcgctggagt tgaagaggtg atccaccaaa tcctcttcga tccgaatggt gtgggccagc
     3121 agataggcca taaacagctc ggcgaaggta ggctcctcgt gcagcacgag gagcatcgcc
     3181 tccttgtcga tgcgcacgat actggagtct tccacggccg tcgcggtcgc cgtccgaatg
     3241 gcctgtccgg cgaggcatga ttcaccaaaa aatgcacccc gttccagaat cgcgataacg
     3301 gcctctttgc cttgctgcga aaccaccgtc agcttcacct tgccggcctg gacatagaac
     3361 actgcttcag cggcatcccc ttgcgaaaag atcgtgtgtt tcttagggga ttccagagta
     3421 gttttcccct tgcccacttt ggtgagaaaa agctggggat caaaggtcgt acgcgcgcgt
     3481 gccatggctc aacctccgct gactaagtgt gattccactc atactgacac gcagagagtg
     3541 actattcttg cacccggacg tggcaatagc aaataggcct tagaccaatt gctgaccgcg
     3601 tttgaccacc cctgtgtcat tatcgagagc ggttcagcga atacgacgcg cccggcgttc
     3661 gcacgggtgc ttgcaaatcc tccacgtatt gttgcagttc gaccagtgcg tgattgagtt
     3721 gtgtcaccga gcagtggcgc aaggtatgaa tgctcttacc tgtccacagc cgaacggcct
     3781 ttcgagacgg tgaatttagc gtcaacacat aggtctgggg ggtcgtcttc atcatgtcct
     3841 ccatttgcgt ctttgctcgt agttttgaca tcggcccggc atgcagcggc ttccccggag
     3901 catgtgtcac gcggctcgaa aaacagcaag cagatcactc aatgccagtg tcttgtgttt
     3961 caagaccgct cgctcggctt tcagttcatc gagggcatct gccttcgcgg ctccatcctt
     4021 cagacatgcc atgacaaggg tttctatccg ttcgcactcc tgcgcgacct tggccttcac
     4081 cacctggttc accgacagca tgtccagcac cgcggctttg gtcgaggcta gagtggcttg
     4141 gaattgtggc tccggatagc tgatgcgtgc cgcctcgctc acacagcggt ccaagctttg
     4201 cgccagaaag agatataatc gcgacagcgt gtcagtcatg tcaatgtggc tctgaacagt
     4261 gggttggacg ttcattgctc atctcctttt cgttttaatc cagcctgatg atcttcttta
     4321 tcccgcgcta ccaggctgct tcgccgtagt tgagtctccc gcgattgcca tcggttcggt
     4381 ctccttctgc atcgatattt tgagttcatg tatggccgtt gctacatcct gctccgtcga
     4441 tccgaaccga tcgcccatga ccttgatcgc ttgtcggatg gctgtcgtca tcttcgtgat
     4501 ctgctcttgt ggtgtcatgc aatctcctgt tagtgcccta tgagtaaaac cttgcccggc
     4561 gatctgctac cgacataacc gactatcgtg caacacccaa gagcagggac cctatggggc
     4621 acgccgtttt tcggcatcac tttcactcgc catcgacgag gctaataccc acacgagcat
     4681 gcagagcgta ccgagtccaa ttatctcgat catagaacac ctctcgtgcc actcgctcaa
     4741 attgtggtgg tatccctaca acaatttggg aggcaccagt gcccgaacca gaccattctg
     4801 tggaacctcc gcccgtctgg aggacagcac gaagagaccc gtcgtaaaca gactgatgag
     4861 aatgagcgac gccacgagaa aggtcgtcgt cgtcgaaatc ctgggcaacg cgtcctgtgc
     4921 gctcagggcc gcaactcgat ccgccgttaa acggtcagaa gttacgcccc gctgttggga
     4981 cccaggcatg gttgcgacta ccgttgcgct cccaagttgt tcctgaatcg ccgctcgaac
     5041 tggttcgtac acctcttgga ctgtggccat ttctaagatt gccgttccca gttgctcttg
     5101 cctcaatgtt gagcgcgttt cgttctcttg ggtcgcggca acaatccctc gaccaatatt
     5161 cggctgccaa ttcgcgagga acaagcggtc catgttctgt cccatggcct ttgtcacacc
     5221 gatcattttg gggttgtcgt gagcagcgcg ttccacggaa gaccacagtc cactgcgaac
     5281 tccacgacgt gtaaaattga cgatcgagcg acccatgact gtctgaactc gggcggtatg
     5341 ctcggattca gcccacgagg cgccggtggt aactgggtcg agatagctat aagggaaatg
     5401 ttgccactgg ttcctttcga aggtcagacc accaagttgt gtgatggctg cagccgcgtc
     5461 tttcgcatgc ctacgttcaa ggagcacatg atccacaata gcttggccga gcaccggttg
     5521 gagcaaatcc atccccgtaa gattgtcggt gggctgtcct atcgtccctg ttcctacgct
     5581 cccgatctgc atgccattgg cagccatgag caacacaccg ctggccacaa tcgtggcgca
     5641 caatcctacc acgacgatta cgtccaaaaa ctcgaatctc cgttgcatag gcacctccac
     5701 gctcctcgat ttactcttgg gcttggccgg gagttcctgt ccctccccgc caagccctcg
     5761 gtctcgccag gtctcgtacg cctagttgtg gtcttcaagt cctctacttg gtcttccgct
     5821 cggtttcttt ccccgaaggc tcggtcttcg cgttcgctcc gaccacaggg gattgaccgg
     5881 ctccatcacg ctccgagatc ctggcattgt tctgattcac cgtgttctga tccatgaacg
     5941 tcggagtcat attcgtatcg ccggacttcg cggcctccat gagggattga ctatgggtca
     6001 gattttgctg gcccgtatcg ttcgcaaact cgccaccaga cggataccca ggatgcgttg
     6061 gcagcatcga tgggttggcc agggcaaggc ccgtcattcc gacaaacata agacccgcta
     6121 ttgtcatcgt gaacagtctc ataatagaac cccctttgtt gcacacatca tcttctcgac
     6181 aaacgatgat ctcattttga tcgcgcaagc gccttatgcg aggactgtca actctctcgg
     6241 acggtctagt tttccgtgcg gtgactggaa tcgctcgtgc taagagagga acggaagcgc
     6301 aaggcagtca gcgtaatcga agcgtgagaa caacataccg gtctctatac cgggatactg
     6361 ttctatatcg aacactatgc cccctgtgcc tggctggaaa gtggcatttc cggcatgcag
     6421 caacgggtgg gcgcttggat ggtgctgctc attcgttgcg ggttagctcg aaagcatgtg
     6481 tgtcaagacg agcgcaatca cccaggctat gacgtaaaac cccaggaata cttctgaccg
     6541 tccatcggtc caaatgcgca tggtcagcct ctcgatctcc ggaattctta cttttctctc
     6601 atcgatgacc gcactgcgtg agggtcattg tccgggccgg tgtaggagac atacatctca
     6661 tcgataatat cttttgcttc tttggaaact gcgaactcca caaaggatgt tgccgtagag
     6721 ttggcttcgt cttttcgaag caatagaacc ggcctgcgca agcgataccg cccatctctg
     6781 acggtctgtt tcgccggttc aacttgatcg acactcaaga gtgccacacc aacgccatag
     6841 gtgaccgcat caagcgctac gccaagggac gcataggtca ctgccgaccc gttgccggca
     6901 aggcggctga tcgctcgctg atcagaccgg ctcacataag cagttggagg actttttcca
     6961 acgatgtcca ggaccgtctc aaacgtgccg cgaatcggct ggttggaagg gctgttgatg
     7021 agttcaattc ttgtatcgtt gccacccaca tccgaccatc gagtcgcggt gcccgagaac
     7081 atcgccgcaa gctgcacaga ggtgatctcc cgcacgggat tcgtaaaatc caccagcacc
     7141 gcaatgccat cccaagcaat gggagtgacc aggaggtccg gatcggtctg accgaccacg
     7201 gccacatctg cctcgcccgt cttcaccatg tgagatggct gaaacgacgg atcccatcgg
     7261 atctcagcta cagcgccaaa atatttcttt tcaaacgcgc tcgtcagccg ttcgaccatg
     7321 cgaagttccg gaccgtttcc ggcaattgtg atggcggcag acaatgcctc gtccgctgac
     7381 caccccaaga atgcaagtgc cacgagcagg cttgcggcca tcccggcatg accaggcctg
     7441 cggcatctct ccacgtctgt ctctcgcctc tcaacgatgt tcatgcctca atcttccttt
     7501 tgtgtaccgg gcggtgggag ctccggggac acgtgcacgg ttctgaaccg gaggggagtg
     7561 ccccgtccgg ttgccttcaa tgacgggatc aatattttca ccgcagagaa ggcagcgcca
     7621 cccatctatc acgtcggagc tccacagctc cctcaactgg gtggtaatca tgaacccctt
     7681 gcagcgcgga caacgcatcg acatcggatc tattttccct tcccgcagat ctcaacaatc
     7741 gcttgtacgg ttccatcttg cggactgatc atttggagca tccccgtgtg ttccggcgct
     7801 cgcttgaaga atgtggcaaa atcttcgtca tacggattct ccaccgtagt cgtgtcgccg
     7861 ctctgacggt tatagactgt ttcatggacg agcgcggaaa ctgtctgcgt gccattggca
     7921 cgtatgggac ttgtgaccct ccagaatatg ggccgctggt tcaatgtacc cctcactccg
     7981 tcgaccgcga tgctcgaaca cagcctggtt ccctttttga cctccacacg atcaccttta
     8041 tcgctaaact gagcgaggcc tgctgccggc actgcgatga ccatcgctga cacaagcagg
     8101 tgtaggaatt tgggcatcac acacctccgt ttcgggacat agggaccaac tcacgcattc
     8161 tgctctttaa ctcacggctg ccggaactgc ccgccgcacg agatacaccg cgacaaccaa
     8221 ggcgaggacg acaagcacat tcgcccccag atcaagtaga taccggtgaa acacttggtc
     8281 gctgagattc tccatgtagg caatctgcgc gccagtcgtg agaatacgtc tggtggatgc
     8341 aatgacacaa atatacagga aaggttctaa cgtgatgact ttggttttga gaaaattaat
     8401 gatggtgcgg aacaattcta agaggataat gaccaataga agatcatgaa cgagcgcgag
     8461 cacggaggga acggcttgcg atctcactga aaggaggaag acataccagg agtgagcgaa
     8521 taccaccatg gcgatcagga gaaaactgaa tccggttgtg atgtagccca ggccgtccaa
     8581 tgtttccatc gcaccgatgg tctttgcatc ggtggtcgga ttcgtgttcg tccacccgct
     8641 cctcaaacgc agtaaagtgg tcacttctgt ccccttctta tctgtaagct tgctcgaacg
     8701 ccctcagaag accagcacga cctactcatc gtacgtcatc ttgatcctca ggcatcgagg
     8761 cccagatcgc tatcccccac gtcatcacca atatggccaa taggctgtat atctgataca
     8821 tgtcagtccc ctttcactcc gagtggttct aattgtcttg tagcctcgtc tgcatggcag
     8881 acctgctgag ccagcatccg ctgctctgga gtgacccgtt cggaaatcct cgctaaacag
     8941 gcttccagtg tgtcctccga aatgtttccc atgctgctgc gcccttccgc tggagccaga
     9001 atcatggctc cctcgacgag cctgtcttcc gcatccacag acgatacggt tgctcccaac
     9061 atgaatgtga cgctcgaaag caacacgagc cgcttcatcg tgaggcagac tttcatttca
     9121 tgctcccttc cttcgggata attcgatagc gggcgtactt ccgtcatcgc ataaagacaa
     9181 tctgaacgcg atgtcgcaaa atcaactccc aggaggagcg gcggagactg gagaatcaga
     9241 tcgcgcttag gaaggtgaga gtggcgcgag agatagggca gtcggcgtag ctcaatacag
     9301 gtttcacctt acagcccggc atcccagcag tcaaatgcca gaaagtgcgt ttgcacgcta
     9361 acaaactgat acagcacagt tataattatt tccttgggac gttctcttat gactgtccgc
     9421 gtgacgaagc aatcggcttc tgctcaattt tacttcgctg ggtgctcagg atctttgtaa
     9481 ccggcataat tccctgtcca atgggaatac cggcggttgg cccagatggt cacctcctct
     9541 ttgtgcatct cgccatatcg cctctgcaac accgagccga acttaacgag atcgccctgg
     9601 atctcgtcga gatcctccgt cgtaatcttc tcccatttcg ctttcagcgg atccctcagt
     9661 tgtaaccaaa actggccgaa ctgttcctga ttcataacgt gctccttggt aaggggatga
     9721 atctttcctc gcttccttgc tacaaaccac ttcgacaggt gcgaagctgg aaatcagctt
     9781 ctcttcaaca aaatggtcgc cgctacagta tgcgcacttg ctattcagct tggccgaacc
     9841 cgagggcctc tgccactctt tccgctttct taaggtgaac tgtcagaacc ggtagtatgc
     9901 cttccgccca ttgcttcacc ttttcattac gtagcatcgc cgcattcttc tcaaactctt
     9961 tgaggtcctt tcgatgattc tcaagcatgt acgcaacaaa tgcttcgtcg aattcttttc
    10021 cggacaaatg ggacaatcgc tgctgttgtt ttttatgttc gctgtcgagc tgaacaggaa
    10081 gataaatccc ttcactagcg gacagttctt ttacttcctg actcgcatac tggtggtctt
    10141 cactaatctc ggccccgaat tccttcacat ctttgtgaga agctttcttg agtgctagcg
    10201 tcccgagtgc aatgtccgcc aactgtcctt gggcagcctt ttgcaggaac gccgtctcct
    10261 tggcatctag ctttcgagcg gcttgaagcg acgccgtggt gagaaatgta gcgacgagta
    10321 ctgcgacaaa cacgtaacga gccatggcac ctccggggga tttatggttg caactctgta
    10381 ctatggtcca gccagtccat gccaggaact aggaatcgcc taatagtaag cgaattctca
    10441 ctgggattaa tggcggtcat ggcgctagga ggctgtgttg gcgcggaaga ccgtttgaga
    10501 agacacaaaa atttataacg ctgtagaacc atccgtttat agggtct
//
LOCUS       DASTQY010000178        17468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_654922, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000178 DASTQY010000000
VERSION     DASTQY010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 17468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17468
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654922"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /gene="nuoF"
                     /locus_tag="VFR82_05835"
     CDS             <1..1270
                     /gene="nuoF"
                     /locus_tag="VFR82_05835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEU4501154.1"
                     /translation="QPGYTGSLADYEQTGGYQSVKKVMGKVSPAEVTQLVIKSGLRGR
                     GGAGFPTGVKWGFLPKDYQGLRYLCCNADESEPGTFKDRQLIERDPHQILEGIVLACY
                     AIAAETAYIYIRGEFVLGARILEEAIGEARRAGYIGKNILGTGVTVEIWVHRGAGAYI
                     CGEETALLESLEGKRGLPRVKPPFPATHGLYNKPTVVNNVETLANLPHIINRGPEWFA
                     AIGSPPKSTGTRIFCVSGHVKRPGNYEVPMGITFRELIYEHAGGMRSEKPLKAFIPGG
                     ASAPFLTPDHLDVKMDFESVAAAGSMLGSGGVTIMEEGTSMVWAALRLMEFFYHESCG
                     KCSPCREGSSWLVQTMRRIVDKRGRAQDLETLTELCKNIAGRTVCAFGDAEVAPIMST
                     LKHWRPEYIALIHEAEAANLLRPQPVGAKG"
     gene            1274..3946
                     /gene="nuoG"
                     /locus_tag="VFR82_05840"
     CDS             1274..3946
                     /gene="nuoG"
                     /locus_tag="VFR82_05840"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEU4501155.1"
                     /translation="MTVSSSQTVRLTIDGMEITVPKGTLVIEAARRVGVMIPHFCYHP
                     KLKPDANCRMCLVEIEKMPKLQTSCSTIATDGMSVRTATSVVNDAHKSVLEFILANHP
                     LDCPVCDQGGKCDLQDFSHEYTATGSRFLETKRIFQKEYFSPLIETQMNRCVQCLRCV
                     RYCDEVMDVKALAPSGRGTMTEIKSFSSHPLDCEFCGGCVQICPVGAITSRLSMYEYR
                     PWMLKRADTICQYCGDGCQITVQTKDNELIEVNSSYGAGRNNGDLCARGFFGFHATSR
                     PDRLTKPLVRRNGVLVEATWEEALEYTAEQIARLKRIHGAQAFGGLTSSRCTNEDLYL
                     FQKFMRLVIGTNNLDSSARYGHLNGVQAMRRVQGTHRWTVTFEDIVEADVLLLVGTSI
                     TETNPVTGLRVKEAVKKRMARLLTIESMQPGIDSISNITNLADQHVGVPTAQFGNALS
                     GLLKAVMEGGHVAPSVQKESPEYVMALSQVLERLAWTDLEAATGASPDSFRQMAAAIA
                     KSHRFVVLTGQDLLRSVGGYGACLQLLDLLVLTGKSTEAGCGFAPLAEENNDQGAVEL
                     GAVAELLPGALDLASSDARAKIGLAWNTDIPSTPGATLTDMIKRARAGHLKAMLVVGE
                     NPIGTLPSHMGAREACQSLELLICQELFMTETAALAHVVFPAAAPFEKAGTWTNEEGH
                     VQAIRPAVEPAGESRPDWEILSALSLLLGTPMEYNDSKDLLKEIRSVIPGYGLLGPTP
                     TPQKVDRGAVDRYVSGGYRDDLSTRYTLAPHGPRLDGCDWYMHLTQSLFHSGKLSTRS
                     KGLLEIESRDAIRMNRADGTRLALADGDRVRLSNSRGEMTTSVKLVERVPQGTVWFPD
                     HFAQEATKLFDCTIDQATKVPAFRTTSVAITKVR"
     gene            4110..5183
                     /gene="nuoH"
                     /locus_tag="VFR82_05845"
     CDS             4110..5183
                     /gene="nuoH"
                     /locus_tag="VFR82_05845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEU4501156.1"
                     /translation="MTELSLRLAVSVAQIAAVMGVVMLTVMILTLAERKVLGWMQDRM
                     GPMEVGPYGILQPIADGLKLFFKEDIVPAGANKFMFSLAPILAMVPALIGFAVVPFGP
                     SHKVELFGISIEPFVISDINIGILYILAFTSIGAYGIILGGWASNSKYSLLGGLRSAA
                     QVISYELNVGLAIVGVLILAGSLSLVKITEAQAGGFWHWFVFALPAPQIFAFVVYVIS
                     AVAETNRVPFDLPEAESELVAGFFTEYSGMRFAFFFIAEYANMVLVSCVAAAMFLGGW
                     NAPYPGTILAHLGLEQVAWIENLAWFTVKAYAFLFLFFWLRATLPRLRYDQLMKFGWK
                     VMLPIALGNIVVTSIAVYLFPRG"
     gene            5216..5758
                     /gene="nuoI"
                     /locus_tag="VFR82_05850"
     CDS             5216..5758
                     /gene="nuoI"
                     /locus_tag="VFR82_05850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEU4501157.1"
                     /translation="MKNVVAWLKTIVFYEILVGMKATMSHLLHYKPITVQYPHEKRTL
                     PDNYRGMLGLLRYDDGTEKCVGCDLCEAACPSRVIRVVSAEVPAEPTKRYAKEYYMDM
                     TRCLFCGMCVDACPVDALGMSREFEWSVYDKRQLHLNKEQLLAIGDRAFPVREKRLEL
                     QHPNVAFFNVSFGHVPQKPN"
     gene            5844..6359
                     /locus_tag="VFR82_05855"
     CDS             5844..6359
                     /locus_tag="VFR82_05855"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU4501158.1"
                     /translation="MDQVFFFYFALVIAASSVLVVAMRNPIYSALSLLIMFFHVAGLY
                     VTLHAEFLAAVQVIVYAGAILVLYLFVVMLLNLKQDDRYHRQWPVAAAIGSVLVIEAI
                     VLTLLRGGTAPAVRPGQESEAAGLGNTEALGDVLYSTYLFPFEVASLILLVAMIGAII
                     LAKKDLEGRQA"
     gene            6387..6692
                     /gene="nuoK"
                     /locus_tag="VFR82_05860"
     CDS             6387..6692
                     /gene="nuoK"
                     /locus_tag="VFR82_05860"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEU4501159.1"
                     /translation="MTVPLSYYVALSAVVFLTGLLGVLVRRNVIIILLSVELMLNATN
                     INFVAFSQALHNIAGQVFVFFALTVAAAEVAVGLAIIIALHRAKATINVDEFQLLKW"
     gene            6692..8593
                     /gene="nuoL"
                     /locus_tag="VFR82_05865"
     CDS             6692..8593
                     /gene="nuoL"
                     /locus_tag="VFR82_05865"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU4501160.1"
                     /translation="MMTYELIPLLPLASFLILGLLGHWIRGNAHFVAVPAMLASFLLS
                     IFAFMEVAGGHESTLRLYTWVTSGILDVHIGIAIDRLTAVMLLLVTTVSTLVHIYTIG
                     YMRGDPGYARFFSYIALFTFSMLMLVLADNFLQLFVFWEAVGLCSYLLIGHWYERPSA
                     CVAATKAFIVNRVGDFGFLLGLLLVLTTFNSLDYSDVFAHTTSHSRELINLLEPFGGS
                     LEVSVITMICLLLFMGAIGKSAQVPLHVWLPDAMEGPTPISALIHAATMVTAGVFMVA
                     RLAPLYDAAPAARDVVAVVGALTMILGATIALTQTDIKRVVAYSTMSQLGYMMMACGL
                     GAYAAGIYHLLTHGAFKALLFLGCGSVIIALHHEQDMRRMGGLKDKLPVTYWTFVVGS
                     LALAGFPLTAGFFSKDDLLISAWSAGILGQVLTAFGLVTALMTAFYTFRLVFVTFWGP
                     FRGDRHHADHVHEPSYSMTVPLIILAVLSIVTGYVGIPDFLEPVFAREGAASHQEGSG
                     ATAVMLLATLMGLLGIAGAYYTYVMDPSLPDRWAQRWQAAYQLSLNKWYVDEVYDRLF
                     VRPTFKAAVELWKRMDVATIDAAVNGVARAIAWGGWLLRLVQSGQTQHYALGMALGAV
                     VILTLLMVM"
     gene            8599..10155
                     /locus_tag="VFR82_05870"
     CDS             8599..10155
                     /locus_tag="VFR82_05870"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU4501161.1"
                     /translation="MHTGGFPWLTVLIFLPLAGAGALYLVKESHARAIALVITVADFL
                     VALPLWWLFDSSLSHMQFVEHVQWITSPPIHYSLGVDGISLPLVLMTALLMPLCVLIS
                     WESITTRVRTFMAVLLVMEGAMIGVFVALDFVLFYVFWEAMLIPMYLLIGVWGGPNRL
                     YAAMKFFLYTLVGSMLLLVAVLVLYFYGGHTFDILALSQVTYPASLQMWLFWAFFAAF
                     AVKVPMFPFHTWLPDAHVEAPTAGSVILASVLLKMGAYGFVRFSLPMLPDASRIMTPL
                     MAALSIAAIIYGAYMALAQVDLKKLIAYSSVSHMGFVTLGLFVMNQEGIEGAVMQMVN
                     HGITTGALFLCVGMIYDRTHSRLISDNAGLAKPMPRYATCLVIFSLSSLGLPGTNSFV
                     GEFLVLVGTFLWSKAATAIASLGIILAAVYLLYMVQRVAFGTPSARELPRLQDIDYRE
                     FGTLVPLVALVFWIGLFPNPLVSRMHASVTNTLETMSRTRVAPTSHPSATHRPPVVLT
                     ATGHAQADRP"
     gene            10152..11639
                     /locus_tag="VFR82_05875"
     CDS             10152..11639
                     /locus_tag="VFR82_05875"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEU4501162.1"
                     /translation="MTSYGSDLLVILPELIVVAAACLVFVLDPLTPHSRKELLAWLSL
                     GSLALCIGLTGGQISVLNLRLPAFSDLVVVDGYARFWKLLLYGVTGLTILMSLPYLKA
                     EGLHLGEYYGFILLALSGMMVMVSGADLLTIYLGTELMSLSLYVMAGLKRASPRSLEA
                     SAKYFVLGAFSSGLLLYGISLLYGLTGSTRLSAIADALGTKGFDDPLLLIATILLIVG
                     FGFKLAVVPFHMWTPDVYQGAPTAVTAFMAVASKAASFAAFLRVFVEGLGGLRGDWSN
                     LLIGLCVITLVLGNVVAIVQTNIKRMLAYSSIAHAGYALIGLVAAGRAAGESGGTSGI
                     ASVMLYLTFYAFMTLGAFAVIAMFRKGGLEGEDIDDYAGLAKRQPVAALMMLVFMVSL
                     AGIPPTAGFLGKFYVFMAAVEAGLSWLAAVALIFAAVSAYYYMRVVMLMYMREPEAAV
                     IDSPHLVTSPALSIVLACAVAGVILLGLYPNPVVSLAIQSVLMLR"
     gene            11727..12620
                     /locus_tag="VFR82_05880"
     CDS             11727..12620
                     /locus_tag="VFR82_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU4501163.1"
                     /translation="MPMLMSFWRLLADLTRSFLRHGCASLAASLAFFSLLSLFPLVFM
                     LLYGVSFIVSHEVIGGQVLLSFLKGFLPTLGERLAVELQRVSELETVRWLVFLSFAWF
                     GTLVFYELDYALNVVFDSTWKRHPLISTTIAVASQGAVALMLVLSYVATQTVNFLTAY
                     APHLWGLDLIALAAHDVFLTYTLPFTMAFLTVGALYRYVPRRRPQWREAMIGSLTFSL
                     LWVAAKLIFVTYNEYATVYTKLYGSLLEVILLLLWIYYSALLLLLGAVIAHVLQQRSQ
                     ASAAGNFSPARMVSPSAHAGG"
     gene            12705..14060
                     /locus_tag="VFR82_05885"
     CDS             12705..14060
                     /locus_tag="VFR82_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501164.1"
                     /translation="MPEDMGKEMLMLGGTLIGFIAALLTVMEKLMDIQHRLTSKKERK
                     PSVSHERQTQDPLATIDFFSTKPLRGTSYLLMYETGVIVAAGLMLNYAGLMLSRHLDS
                     ILFLDMTGTALVAFLLGPWWGAIVALLSNSVVNWLLFPESGADVVIFPWSLVSMTGAL
                     YWGWMAREAGFQKYLRTGKSTVLSHAWFLSIFGAGGAVVMSLPGTFVQAALNEHATFA
                     LNPAVAETLHARVLEWEQMVHQYLESVFGLTWGEHVGWWVVNWFQNCVRYVPDKTMSA
                     AVALVVLKYGYPLFERELIHGGPDGERPQDEQLLPLVLGLLYAPSFASLVSSEMYGGG
                     AFWPIWTLPWVGILGGYFYLRYWGATASTLNEARLQRAERYARALKPIGKEASYEFCR
                     RLTFMTLIASLLFALCLPIILMDFYRVTFKFFCVVYGFLLVVHLVRIAIAQNISIARA
                     E"
     gene            complement(14088..15239)
                     /locus_tag="VFR82_05890"
     CDS             complement(14088..15239)
                     /locus_tag="VFR82_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU4501165.1"
                     /translation="MTLPSWEIGRALGIPIRVHASWFLVFVFVTWTLATEYLPGALPG
                     LSDSRYWGMGAVAALLLFLSVLLHELGHSYVAQRYRIPIGQITLFLFGGVAQMRAEPP
                     SPKAEFLIAIAGPAVSFALGAFCLGTAVAADWLSLPSGGQGFVVLGGLLGVVNVQLGL
                     FNLIPGFPLDGGRALRAGLWAWGRDFHWATSRAALLGLGFGVLLASVGAGLIGGAISG
                     MLHPSTASNGGWLMFIGAFLFGAAWSTRKQASLRFALNATSVRDVMIRAVVTIPSQLS
                     LQAAVDEFFVAYGYGSFPVVEEGRVVGIIAVQDVQAISQGLWTWRTVADVMRSASPEL
                     FIQPEWSIMQAMEHMVRTGFDRLVVMEGGRPVGLITGSAVARFLQLHKA"
     gene            15340..16041
                     /locus_tag="VFR82_05895"
     CDS             15340..16041
                     /locus_tag="VFR82_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU4501166.1"
                     /translation="MRRVHLGLVTVLAALTLAAATDARAQVIKSGPPACPTIALTFDL
                     CPVRKGAGYDKALIEYLIDHNIPATFFMSGQWIAKHDPEVEQLLGIAFFEVGTHGEVH
                     AHLPMHNAEEQRVEILGPVKLLSEHYAHDTVLFRPPFGEYNEVTVDVVKALGLRLVQW
                     NIESGDPDPSLSAEHILARVEKRAKAGSIVVLHANGKGKHTRQIVERLTEDILPRKGL
                     KPTTVSDLLSCKPVK"
     gene            16023..16520
                     /locus_tag="VFR82_05900"
     CDS             16023..16520
                     /locus_tag="VFR82_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4501167.1"
                     /translation="MQTREVMSGRLIRPMRADDCDTVITLLGDSDPWKTLGYSREDWK
                     GIFCPTPQGRECFVAEAEGRIAGIAIVRQKFLLGDYLELLGVAAWARNQGAGKQLIGH
                     VESLVFARTKNLFACVSDFNKGAREFYRKQGYHEIGPMPNFLIPGSAEILLRKTAGPA
                     REQSR"
     gene            16596..17003
                     /locus_tag="VFR82_05905"
     CDS             16596..17003
                     /locus_tag="VFR82_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4501168.1"
                     /translation="MTDFKVTKLLHTRMRVSDMDQTIAFYTGVLGLEVLERKTSPRGS
                     HLAFLRVPDSEELIELCSFPPSGAVKVQPDLVHLAFQVENLDRTMTALQQKGIPITDG
                     PTTTSSGSRFVFIDAPDGYEVELIERPAGAKIV"
     gene            17174..>17468
                     /locus_tag="VFR82_05910"
     CDS             17174..>17468
                     /locus_tag="VFR82_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEU4501169.1"
                     /translation="MKNGFFRGHGLGNDYLVMDPKELSFKLTPSTIAALCDRNWGLGS
                     DGILAAASSTKADFGLRIFNPDGSEAEKSGNGLRIFARYLHATGKTKKKHFTVE"
BASE COUNT         3418 a         5083 c         5007 g         3960 t
ORIGIN      
        1 tcagcccggt tataccggct cgttggccga ctatgaacaa acggggggct accagtcggt
       61 caaaaaagtc atggggaagg tgtcccccgc ggaggtgacg cagctagtca ttaagtccgg
      121 gctacgtggc cgtgggggcg ccggatttcc aactggagtg aaatggggct ttctacccaa
      181 agattaccaa gggttgcggt atctctgctg caatgcggac gaaagcgagc cggggacgtt
      241 caaggatcgg cagttgatcg aacgcgatcc tcatcaaatt cttgaaggaa tcgtgctggc
      301 ctgctatgca atcgcagcgg aaacggccta catctatatt cgaggcgagt tcgtgctcgg
      361 tgcgcgaatt cttgaggagg ccattggcga agctcgccgg gccggctaca tcggcaagaa
      421 tatcctgggc acaggcgtga cggtcgaaat ctgggtgcat cgcggtgcag gcgcctacat
      481 ttgcggcgaa gagacggcgc tgctggagtc gctggagggg aaacgaggtc tgccacgagt
      541 caagccgccg tttccggcca cacacgggct gtacaacaaa ccgaccgtgg tgaacaacgt
      601 cgaaacgctc gccaacctgc ctcatatcat caaccgtggg cctgaatggt ttgcagcaat
      661 cgggtcaccg cccaagagca cggggacgcg catcttctgt gtcagtggac acgtgaaaag
      721 acccggcaat tacgaagtgc caatgggcat aacgttccgg gaattaattt atgaacacgc
      781 gggcggaatg cggtccgaga aaccgttgaa agcgttcatt cccggcggtg cgtctgcgcc
      841 ttttctgaca cccgatcacc ttgatgtaaa gatggacttc gaatcggtcg cagcagctgg
      901 ttcgatgttg ggttccgggg gcgtcaccat catggaagaa ggcaccagca tggtgtgggc
      961 tgcgctgcgc ctcatggaat ttttctatca tgagtcgtgc ggcaagtgta gtccatgccg
     1021 ggagggcagc tcctggctgg tacagaccat gcgccggatc gtggacaaac gaggccgggc
     1081 tcaggacctc gaaaccttga cggagctttg caagaacatc gccggtcgca cggtgtgcgc
     1141 cttcggagac gccgaagtcg caccgatcat gagcacatta aaacattggc ggccggaata
     1201 catcgcactc atccatgagg cggaagccgc gaatctgctc cgtccgcagc cagtaggggc
     1261 caaggggtaa ggcatgaccg tgtcttcttc acaaacggtt cggttgacga ttgacggcat
     1321 ggaaatcacc gtgccgaagg gcacgctcgt catcgaagca gcccggcgtg tgggagtgat
     1381 gatcccccac ttctgttatc acccgaaact aaagccggac gccaattgcc gcatgtgtct
     1441 cgtcgaaatc gagaagatgc ccaagctgca gacctcctgc agcacgatcg cgacggatgg
     1501 catgtcggtg cggacagcca ccagcgtcgt gaacgatgcg cacaaatcgg tgctcgaatt
     1561 catcctggcc aaccatccgc tcgactgtcc cgtttgcgac cagggcggca aatgcgacct
     1621 acaagacttt tcccacgaat acacggctac cggcagccgt tttctcgaaa ccaaacgcat
     1681 tttccagaaa gagtacttta gtccgctcat cgaaacgcag atgaaccgct gcgtccaatg
     1741 cctgcgttgt gtccgctatt gcgacgaggt gatggacgtc aaagccctgg ctccttccgg
     1801 gcgcggcacc atgaccgaga tcaaatcctt cagttcccat ccgctggact gcgagttctg
     1861 cgggggctgt gtgcagatct gtccagtcgg cgcgatcacc agtcgcttgt ccatgtacga
     1921 atatcgtccc tggatgctga agcgcgccga taccatttgc caatattgcg gcgacggctg
     1981 tcagatcacc gtgcagacca aggacaacga gctcatagag gtcaattcgt cctacggcgc
     2041 cggccgcaac aacggcgatc tctgcgcgcg cggctttttc gggtttcatg ccacgagccg
     2101 gccggatcgg ctgaccaagc cgctcgtccg ccgaaacggt gtcctcgtcg aggcgacgtg
     2161 ggaagaggct ctcgaataca cggcggagca aattgcgcgg ctcaagcgga tccatggagc
     2221 ccaggctttc ggcggtctga cgtccagccg ctgcacaaac gaagatctct atttgtttca
     2281 aaagttcatg cgactggtga tcgggacgaa caacctggac agcagcgcac gctacgggca
     2341 cctgaacggg gtacaggcga tgcggcgcgt tcaaggcacc caccgctgga cggtgacttt
     2401 cgaggatatc gtcgaggccg atgtccttct cctcgtcggc accagcatca cggaaaccaa
     2461 tcccgtgacg ggtctcagag tcaaggaagc ggtcaaaaaa cgtatggctc gcctcttgac
     2521 catcgagtca atgcaacctg ggatcgactc catcagcaac atcacgaatc ttgcggatca
     2581 acatgtgggc gtgccgacgg cccagttcgg gaacgcgttg agcggcctcc tcaaggccgt
     2641 gatggagggc ggccacgtcg ccccttccgt gcagaaggaa tcgccagaat atgtgatggc
     2701 gctgtcacag gtgcttgaac gcctcgcttg gactgacctc gaggccgcga cgggagcgag
     2761 tcctgacagc ttcaggcaga tggcagccgc gatcgccaaa agtcatcgct tcgtcgtgct
     2821 cactggccag gacctgttgc gaagcgtggg cggttatggt gcctgccttc agcttcttga
     2881 cctgctggtg ctcacaggta aatcgaccga agcaggctgc ggctttgctc cattggccga
     2941 agaaaacaac gatcagggcg cagtggagct gggagcggtt gctgaattgt tgcctggtgc
     3001 actcgacctc gccagttcgg acgccagagc gaagatcggc ctcgcgtgga acacggacat
     3061 cccctccaca cctggcgcga ccttgaccga catgatcaag cgtgctcggg ccggccactt
     3121 gaaagccatg cttgtggtgg gcgaaaaccc gatcggcact ttgccatcgc acatgggcgc
     3181 acgagaggcc tgccagagct tggagctcct catctgccag gaacttttta tgacggaaac
     3241 cgcagccctc gcccatgtgg tgttccctgc cgccgccccc tttgagaaag ccggcacatg
     3301 gacaaacgag gagggccatg ttcaagccat acgccccgcg gtcgagccgg caggtgaaag
     3361 ccggcctgat tgggaaatat tgtccgcgct ctctctgttg ttggggacgc caatggagta
     3421 caacgacagc aaggatttgc tgaaagagat ccgcagtgtc attccgggct atggcctcct
     3481 cggtccgaca cccactcctc aaaaagtgga ccggggagct gtcgatcgat acgtgtcggg
     3541 gggctatcgc gacgacctct ccactcggta tactttggcc ccgcatggac cgcgccttga
     3601 tggatgtgac tggtacatgc acctgactca gagcttgttt cactcgggga aactatccac
     3661 ccgatcgaag ggattacttg agatcgaatc ccgcgacgcc atccgcatga accgggcaga
     3721 tggaacgcgg ttggccctcg ccgacggtga tcgcgtccgg ctgtcgaatt cacgaggcga
     3781 gatgacgacc tccgtcaaac tcgtggaacg tgttccgcag ggaacggtct ggtttcccga
     3841 tcatttcgct caagaggcga ccaagctttt cgactgtacg attgaccagg cgacgaaagt
     3901 acccgccttc cggacaacgt cagtcgcgat caccaaggtt cggtgacaag gagaagtgat
     3961 ccaggatgtt caaaaggcac attctcaccc gcccacccct ggcgcttgtt ttcacccacc
     4021 caacccaccg attgtttcgc aatcgatgtc ccgggacgtg ccttggggcg tgcatggccg
     4081 cagcaagaat ttgagtaagg agcacgatcg tgactgaact gagtttgaga ctggcagtct
     4141 cggtggccca aatcgccgcc gtcatgggcg tcgtcatgct gacggtaatg atcttgaccc
     4201 tggccgaacg taaggtgctc ggttggatgc aggaccgtat gggtcccatg gaagtcggtc
     4261 cttacgggat cctgcagccc attgccgacg gcctgaagtt gttcttcaag gaagacatcg
     4321 tgccggccgg cgcgaacaag ttcatgttca gccttgcgcc gattctggcg atggtaccgg
     4381 ccctgatcgg attcgctgtc gtgcccttcg gaccgagtca caaagtcgaa ctgttcggca
     4441 tctcgataga gcccttcgtc atcagcgaca tcaatattgg tattctctac atcctggcat
     4501 tcacgtctat cggcgcctac ggcatcattc tgggtggctg ggcctcgaac agcaaatatt
     4561 ctctcttggg agggctccga tccgccgccc aggtcatcag ctatgaactc aacgtcggcc
     4621 tcgccatcgt cggggtcctc attctcgcgg gctcactcag cctggttaaa atcaccgagg
     4681 cccaagcggg cggcttttgg cattggttcg tgttcgcgct gccggcgcct cagatctttg
     4741 cgttcgtcgt ctatgtcatt tccgcagtgg cagagacgaa ccgggtaccg ttcgacttac
     4801 cggaagcgga aagcgagttg gtcgcgggat tttttacgga atacagcggc atgcgcttcg
     4861 cctttttctt tatcgctgag tacgccaaca tggtcttggt gtcctgtgtc gccgccgcca
     4921 tgtttttggg cggctggaat gcgccctatc cagggacgat actggcgcac ctgggcctgg
     4981 aacaggtggc ctggattgaa aatctcgcat ggttcacggt gaaggcatac gccttcctct
     5041 tcctgttctt ctggctccgg gccactttgc cgcgtctgcg ttacgatcaa ctgatgaaat
     5101 tcggatggaa agtgatgttg ccgattgcct taggtaacat cgtcgtcacc tcgattgccg
     5161 tctatttatt tcccagagga taagcggtga cccgccgcca aaacggatgg cgtagatgaa
     5221 gaacgttgtt gcctggctca aaaccatcgt cttctacgag atactcgtcg gcatgaaagc
     5281 gacgatgtcg catctgctgc actacaagcc gattacggta cagtaccctc acgagaagcg
     5341 tactctcccg gacaactacc gcgggatgct cggtctgttg cgctacgacg acgggacaga
     5401 aaagtgtgtg ggctgcgatc tctgcgaggc ggcctgtcct tcgagggtca tccgcgtcgt
     5461 gagcgcggaa gtcccggcgg aacccacgaa gcgttatgcc aaggaatatt acatggatat
     5521 gacgcgctgc ttattttgcg gtatgtgcgt cgatgcctgc cccgtggatg cgcttggcat
     5581 gagccgcgaa ttcgaatggt cggtctacga caagcgccag ctgcatctga acaaggagca
     5641 gctgttggcg atcggtgacc gtgcgtttcc agttcgcgag aagcggctgg aactgcaaca
     5701 ccccaacgtg gcgtttttca acgtctcatt cggccacgtc ccacagaaac cgaattgaat
     5761 tgcagccatg gccgaccgag cggacgcgga ctgtcgggac gggatgaacg tgcgtgcttt
     5821 gctaacggga atgccggctt gatatggacc aggtcttctt tttttatttt gcgctggtta
     5881 ttgcggcgtc atcggtgttg gttgtcgcga tgcgcaatcc catctacagc gcgctctcgc
     5941 tgctgatcat gtttttccat gtggcgggtc tctatgtgac ccttcatgcc gaattcctgg
     6001 ccgctgttca ggtcatcgtc tatgccgggg ccatcctcgt gctctatttg ttcgtcgtca
     6061 tgctgctgaa tctgaagcag gacgatcgct atcaccggca gtggccggtg gctgcggcaa
     6121 tcggctctgt tttagtcatc gaggccatcg tgctgacgct cctcagagga gggacagcac
     6181 cagccgtccg gccaggacag gagagcgagg ccgccggact tggcaacacc gaagcgttgg
     6241 gtgacgtgct ctactcgacc tatctgtttc cattcgaggt ggcgtcgttg atcctgctgg
     6301 tcgcgatgat cggtgccatt attttggcca agaaggatct tgaaggacga caagcgtgag
     6361 acattgggcg tgacggctgg tgatcgatga cggtgcctct ctcctactac gtcgcattga
     6421 gcgcggtggt ctttctgacc ggactcctcg gcgttctggt cagacgcaac gtcatcatca
     6481 tcttgctgtc ggttgagctg atgttgaacg ccaccaacat caattttgtc gcattctccc
     6541 aagcccttca caacatcgcc ggccaagtct tcgtcttctt tgcgctgacc gtcgccgccg
     6601 ctgaagtcgc tgtcggactc gccatcatca tcgcgctgca ccgggcgaaa gccaccatca
     6661 atgtcgacga gtttcagctg ttgaagtggt gatgatgacc tacgaactca ttcccctcct
     6721 gccgctcgcc tctttcctca ttctagggct gttgggacat tggatcaggg gaaatgccca
     6781 tttcgtggcg gttccagcta tgctcgcctc gttcctcctg tcgatctttg ctttcatgga
     6841 ggtagccggc gggcacgagt ccacccttcg cctctatacc tgggtgacct ccggcatcct
     6901 cgacgtccac atcggaatcg ccatcgatcg cctcaccgcc gtcatgttgc tgttggtcac
     6961 cacggtcagc accctggtcc atatctatac gatcgggtac atgcgtggcg atcctggata
     7021 tgcccgcttc ttcagctata ttgcattgtt taccttctcc atgctcatgt tggtcctcgc
     7081 tgacaatttc ctgcaattgt tcgtcttctg ggaagcggtc ggcctgtgtt cctatttgtt
     7141 gatcggccac tggtacgaac ggccatcagc ctgcgtcgca gcgaccaagg cgttcattgt
     7201 caaccgggtg ggagatttcg gatttctcct tggcctgctg ctggtcttga ccacgttcaa
     7261 tagcctggac tattccgacg tcttcgccca taccacttcg cactcacgag aactcatcaa
     7321 cctccttgaa ccattcggcg gttcgctcga ggtgtcggtc ataacgatga tttgcctgct
     7381 cctctttatg ggcgcgattg gcaaatcggc tcaggtcccc ctccatgtgt ggctgcctga
     7441 tgcaatggaa ggacccacgc caatctccgc cctcattcac gccgcaacga tggtcaccgc
     7501 tggcgtcttc atggtcgcga gactggctcc gctttacgac gctgctcccg ccgccaggga
     7561 cgtcgtagcc gtcgtcggcg cgttgacgat gatactgggg gcaaccatcg cgttgacaca
     7621 aacggacatc aagcgggtgg tcgcctactc caccatgagc caactcggat acatgatgat
     7681 ggcttgcgga ctgggcgcct atgccgccgg gatctatcat ctcttgaccc acggagcctt
     7741 caaagcactg ctgttcctgg gttgcggctc cgttattatt gctctgcacc atgagcagga
     7801 catgcgccgc atgggcgggc tcaaggacaa actgcccgtt acctactgga cattcgtcgt
     7861 cggctcattg gcgctcgccg gatttcccct caccgccggc tttttcagca aagacgatct
     7921 cctgatttca gcctggtcgg cggggatact cggtcaggtg ttgacggcct tcggccttgt
     7981 gacggctctg atgaccgcgt tctatacctt ccggctggtg ttcgttacgt tttgggggcc
     8041 ttttcgggga gaccgtcacc acgcggatca cgtgcatgaa ccctcgtact cgatgacggt
     8101 tccgctcatt atccttgctg tcttgagcat cgtcacaggg tatgtgggca tcccggattt
     8161 tctcgagccg gtctttgcca gggaaggcgc ggcatcgcac caggaaggaa gcggagccac
     8221 ggcagtcatg ctccttgcga ccctgatggg attgctgggg atcgccggcg cttattacac
     8281 atacgtgatg gatccttcac tgcccgatcg ctgggcccag cgttggcagg cggcctacca
     8341 gctgtctctc aacaaatggt acgtagacga agtctacgac cgcctcttcg tgcgccctac
     8401 attcaaggcg gccgtagaat tgtggaaacg catggacgtg gctacgatcg acgcagccgt
     8461 caacggcgtc gcacgggcca ttgcctgggg gggctggcta ctgcgactcg tgcagagcgg
     8521 acagacgcag cattatgcgc tcggcatggc cttaggggct gtcgtgattc tcacgctatt
     8581 gatggtgatg tgaagcttat gcacaccggc ggatttccct ggctcaccgt cttgatcttc
     8641 ctgccgttgg cgggcgccgg ggccctgtat cttgtcaaag agtcgcatgc ccgtgccata
     8701 gccctggtca tcacggtcgc cgactttctc gtggcgcttc ctttgtggtg gcttttcgat
     8761 tcttccttaa gccacatgca gttcgtcgag cacgtgcagt ggatcacatc accgccgatt
     8821 cattacagtc tcggcgtcga cggcatcagt cttcctctcg tgctgatgac ggccctcctc
     8881 atgccgctct gcgtgttgat ttcctgggaa tccatcacaa cgagggtgcg tacgttcatg
     8941 gccgtcctgc tcgtgatgga aggggccatg ataggcgtct ttgtcgccct ggattttgtc
     9001 ctgttttatg tgttctggga agcgatgctg atcccgatgt atttgctcat cggtgtctgg
     9061 ggagggccga accgcctcta tgccgcgatg aagttcttcc tctatacgct ggtcggcagc
     9121 atgctgctgc tcgtcgcagt gctcgtcctg tatttttacg gcggccatac gttcgatatt
     9181 ctggcgctga gtcaggtcac ctatccagcc tcgctgcaga tgtggctatt ttgggcattt
     9241 tttgccgcgt tcgccgtgaa ggtgcccatg tttccgttcc atacatggtt acccgatgcg
     9301 cacgtggaag cgccgaccgc gggcagtgtc atcctggcca gtgtgctgct gaagatgggg
     9361 gcgtacgggt ttgtacgatt cagcttgccg atgctgcccg acgcgagtcg cataatgacg
     9421 ccgctgatgg ctgcgctctc gatcgccgcc atcatttatg gcgcgtatat ggcgctggcc
     9481 caagtcgatc tcaaaaaatt gatcgcgtat tcaagcgtga gtcacatggg cttcgtgacc
     9541 cttggcttgt ttgtgatgaa tcaagagggc atcgaaggcg ctgtcatgca aatggtcaac
     9601 catgggatca ccaccggggc gctcttcctg tgtgtcggga tgatctacga ccgtacgcac
     9661 agcaggctga tctcggacaa tgccggcctg gcgaaaccaa tgcctcggta cgcgacatgc
     9721 ttggtcatct tttccctgtc gtctctcggc ttgccgggca ccaacagctt tgtcggtgaa
     9781 tttctggtgc tcgtcggcac atttctgtgg agcaaagccg cgaccgcgat tgcgtcgctt
     9841 ggaatcattc tcgcagcggt gtatctcctc tatatggtgc aacgggtcgc cttcggcacc
     9901 ccgtctgcgc gagaattgcc gcggttgcag gacattgact accgcgaatt tgggacactg
     9961 gtcccccttg tcgcgctcgt cttctggatc ggcctctttc ctaatccgct ggtcagccgc
    10021 atgcacgcga gcgtgaccaa cacgctggag acgatgtccc gaacaagagt cgctccgaca
    10081 tctcatcctt ccgccactca ccgtccaccc gtcgtcttga ccgcaacggg tcacgcgcag
    10141 gcggaccggc catgacgtcc tacgggagtg atctgctcgt cattcttcca gaacttatcg
    10201 tcgtggctgc ggcctgcctc gtctttgttc tggatccgct gaccccgcac tcacgaaagg
    10261 aactcctggc ctggctcagt ctcgggagct tggccctttg catcggcttg accggcggac
    10321 aaatcagcgt gctgaatctc cgtctaccgg cgttcagcga tctggtcgtc gttgacggct
    10381 acgcccgctt ctggaaactg ctgttgtacg gtgtgaccgg cctcacgatt ctgatgtcgc
    10441 ttccttatct caaggccgaa ggactccatc taggtgagta ctatggcttc atcctcctcg
    10501 ccctttctgg gatgatggtc atggtgtccg gagccgatct gctgacgatc tatctcggca
    10561 cagaattgat gtccctttcg ctctatgtca tggctggact caaacgggcg tcgccgcggt
    10621 cactggaagc ctcggcaaag tactttgtcc tcggggcctt ttcatcaggg ctgctcttat
    10681 acggcatttc cctgctctac ggtctcacgg gcagcactcg gttaagcgcc attgccgacg
    10741 ccctgggcac gaaaggattc gatgatcctc tcctcttgat cgccaccatt cttttgatcg
    10801 tcggattcgg attcaagctc gccgtcgtcc cctttcacat gtggacaccg gatgtgtatc
    10861 agggcgcgcc gacggccgtg acggctttta tggccgttgc ctcaaaagcc gcaagctttg
    10921 ctgcgtttct tcgggtgttc gtcgaagggc tcggaggatt gagaggtgat tggtcaaatc
    10981 tgcttatagg cctctgcgtc atcacgctcg tgctcggcaa cgttgtggcc atcgttcaaa
    11041 ccaacatcaa gcgcatgttg gcctattcaa gcattgccca cgcggggtat gccttgatcg
    11101 gcctggtcgc agcggggcgc gccgcaggag aatcgggagg cacctccggc attgccagcg
    11161 tgatgcttta tctgaccttt tatgccttca tgacgctcgg agcctttgcc gtcatagcca
    11221 tgttccgaaa aggagggctc gaaggcgaag acatcgacga ctatgcgggc ttggccaaac
    11281 gccagccggt ggccgcattg atgatgctgg tcttcatggt atcactggcc ggcattccgc
    11341 caacggcagg gtttcttgga aaattttacg tcttcatggc agccgtggaa gcgggactga
    11401 gctggctagc cgccgtcgcg ctgatttttg cggcggtctc ggcatattac tacatgcggg
    11461 tggtcatgtt gatgtacatg cgagaaccgg aagccgcggt cattgactct ccgcacctcg
    11521 tgacctcgcc ggcgctttcg atcgtcttgg cttgcgccgt cgccggcgtt atcctgctag
    11581 gtctctatcc caatccagtt gtcagtcttg ccattcagtc cgtcctcatg ctccgatagc
    11641 gttcgagcgc gctgtagtcg tgcatgacca ctcaactcgt gctaggctag ggtggccgtt
    11701 ttgatcagtc tacgcagttg gaactcatgc cgatgctcat gtcattttgg cgcttgcttg
    11761 ccgatctgac ccggtccttt ctgcgtcacg ggtgtgccag cctggcagct tcgttggcat
    11821 ttttttcgtt gctctcactg ttcccactgg tgttcatgct gctgtatggc gtcagcttca
    11881 tcgtcagcca tgaggtgatc ggcggacagg tgttgttgag cttcctcaaa gggtttctgc
    11941 ctacgctcgg agaacggctg gccgtagagt tgcagcgggt cagcgagttg gaaactgtcc
    12001 gatggctcgt atttctgtcc tttgcatggt tcggcacgct ggtgttctac gaactggatt
    12061 acgcactgaa cgtcgtgttc gacagcacct ggaaacggca tccgttgatt tccacgacaa
    12121 tcgccgtcgc ctcacaaggc gccgtggcac ttatgctggt gctgtcgtat gtggcgacgc
    12181 aaaccgtcaa ttttctcacc gcctatgcgc cgcatctctg gggattggac ttgattgcgc
    12241 tcgcggcaca cgatgtcttt ctcacctaca cccttccctt tacgatggcc tttctgacag
    12301 tgggcgcact gtaccgctac gttccccgcc gacgcccgca atggcgtgaa gccatgatcg
    12361 gttcattgac gttcagtctc ctgtgggtgg ccgcaaaact catttttgtg acctacaacg
    12421 aatatgcaac cgtttacacc aaattgtatg gctccttgct cgaggtgata ctcctgttgc
    12481 tctggatcta ctattccgct ctgctgctgc tgctcggcgc cgtcatcgct cacgtgctgc
    12541 aacaacggtc tcaggcctcc gcagccggaa acttctcacc ggcaaggatg gtttctccat
    12601 cagcccacgc cggtggctga tttccgtcct gtcgtcttct agcctttttc tggcccagac
    12661 cccgcttcgt cgctagaatg gaccctcaat aagggggacc tgtcatgccc gaagacatgg
    12721 ggaaagagat gctgatgctg ggtgggaccc tcattgggtt catcgccgca ctgctgacag
    12781 tgatggaaaa attgatggac atccaacacc ggttgacctc gaaaaaggaa cgcaagcctt
    12841 ccgtatcaca tgaacgccag acacaggatc cgctggcgac catcgatttc ttttctacca
    12901 agcccttgcg cggcacttcg tatctcctga tgtacgagac aggcgtcatc gttgccgccg
    12961 gtctgatgct caactatgca ggcttgatgc tgagccggca cctcgacagc attctgtttc
    13021 tcgatatgac gggtactgcc ttggtggcct ttctgctcgg cccatggtgg ggagccattg
    13081 tcgccctgct ctcgaattcc gtcgtcaatt ggctgctgtt ccccgagtcg ggagcggatg
    13141 tggtgatatt cccctggtcg ctggtgagca tgacgggggc cctgtactgg ggatggatgg
    13201 ccagagaagc ggggttccaa aaatacttgc gcacgggcaa gagtacggtt ctctcccatg
    13261 cctggtttct gtccatcttc ggtgccggag gtgccgtcgt catgagttta cccggcacct
    13321 tcgtgcaagc agccttgaac gaacatgcca cgttcgcctt gaatcccgcc gtcgcagaaa
    13381 cgctgcatgc cagggtgcta gagtgggagc agatggtcca tcaatatctc gaatcggtat
    13441 tcggattgac gtggggcgag catgtgggct ggtgggtcgt gaactggttt cagaactgcg
    13501 tccgctacgt cccagacaaa accatgagcg ccgcggtcgc actcgtcgtc ctcaagtacg
    13561 gctatccgct gttcgagcgg gaattgatcc acggcggtcc cgatggggag cgcccccagg
    13621 acgaacagct gttgccgttg gtcttggggt tgctctatgc gccgtctttc gcctcgctgg
    13681 tcagcagtga gatgtacgga ggcggcgcct tttggccgat atggactctg ccctgggtcg
    13741 ggatcctggg ggggtatttc tacctgcgat attggggcgc tacggcttca acgctcaacg
    13801 aagccaggtt gcaacgtgcc gaacgctatg cacgcgcgtt gaagccaatc gggaaagagg
    13861 cctcctatga attttgccgg cgattaacct tcatgaccct gatcgccagt ctcctgtttg
    13921 ccttgtgcct gcctatcatt cttatggact tttatcgcgt gacgtttaag tttttctgcg
    13981 tcgtctacgg atttctactc gtggttcatc tcgtgcgtat cgccatcgcc cagaatatct
    14041 cgattgcccg cgccgaatga acgagaaagt gacggcgcca tgcggcgtca cgctttgtgc
    14101 agctgaagaa accgcgcgac ggcggaccct gtgatcagcc ccaccggacg tccgccttcc
    14161 atcaccacca gccgatcgaa acccgttcga accatgtgtt ccatggcctg catgatggac
    14221 cattccggct gaataaacag ctccggcgac gccgatcgca tgacatctgc gaccgtccgc
    14281 caggtccata atccctgaga gatggcctgc acgtcttgga cggcgatgat gccgacgaca
    14341 cggccctcct caaccacggg gaaactgcca tatccgtacg cgacgaagaa ctcatcgaca
    14401 gctgcttgca ggctcagttg cgacggaatc gtcacaaccg ctctaatcat cacgtcccgg
    14461 acggacgtgg cgttcagcgc aaagcgtagc gaggcttgtt tcctggtgct ccaagccgcc
    14521 ccgaaaagga acgcgccgat aaacatgagc catcctccgt tacttgccgt cgagggatga
    14581 agcatgccgg agatggctcc tccgatgagt ccagcgccca cacttgcgag cagaacgcca
    14641 aatcctaatc ccaacagggc cgcccggctc gtcgcccaat gaaaatcgcg gccccaggcc
    14701 catagccctg ctcggagtgc gcgaccgcca tccaagggga aaccgggaat gaggttgaag
    14761 aggcccagtt ggacattgac aactcccaat agtccgccca acacgacaaa accctgcccg
    14821 ccggagggga gcgacaacca gtctgccgcc accgctgttc ccaaacagaa ggcgccgagt
    14881 gcaaaactga ccgcaggccc ggcaatcgcg atcaaaaatt cggcctttgg gctcggcggt
    14941 tcggcccgca tctgcgccac gccgccaaag agaaacagcg tgatctggcc gataggaatg
    15001 cggtaacgct gcgcgacgta ggaatgtccc agctcgtgca agagcacgga gagaaagagc
    15061 agcagtgcgg ccaccgcacc catcccccaa taccgcgagt cggagaggcc gggtaaggca
    15121 cctggcagat attccgtcgc cagggtccag gtcacgaata caaagacgag aaaccatgag
    15181 gcatgcaccc ggatagggat gcccaaggct cgtccgattt cccagcttgg cagagtcatg
    15241 agtgcacagt accagcgctg aaaaccagcc gtcaatgttt cacaactcga tgacatttta
    15301 cggatttagg cgggctgcgg ttcgggtata ctaaagggca tgcggcgagt acacctcggg
    15361 cttgtgacgg tgctggcggc gctcacgctg gcggctgcga ccgacgctcg ggcacaggtc
    15421 atcaagtcgg gacctcctgc ctgcccgacg atcgcactca ccttcgattt atgtccggtg
    15481 agaaagggag cggggtacga caaggcgttg atcgagtatt tgatcgacca caatattccc
    15541 gcgacgtttt tcatgtccgg ccagtggatt gccaagcatg atccggaagt ggagcaactg
    15601 cttggaatcg ccttttttga agtggggacg cacggcgagg tgcatgccca tctgcccatg
    15661 cacaatgcgg aagagcagcg agtagaaatc ctgggcccgg tcaagctgct cagcgaacac
    15721 tatgcgcacg acacggtgct ctttcgtcca ccgttcggtg aatataatga ggtcacggtc
    15781 gatgtcgtga aggccctcgg cctgcgcttg gtccaatgga atatcgaatc cggagatccg
    15841 gatccatcgc tctcagccga acacattctc gcgcgcgttg aaaaacgggc caaagcaggc
    15901 agtatcgtcg tcctccatgc aaacggcaaa gggaaacaca cccggcaaat cgttgaacgg
    15961 cttacggagg acatcctgcc gcgcaaaggg ctgaaaccga cgaccgtcag cgacctgctg
    16021 tcgtgcaaac ccgtgaagtg atgagcggcc ggttgatcag acccatgcgg gcggatgatt
    16081 gcgacaccgt catcacgctg cttggcgatt ccgatccctg gaaaacgctg gggtacagcc
    16141 gggaagactg gaaggggatt ttttgcccaa cccctcaagg ccgggaatgc ttcgtcgcgg
    16201 aagcggaggg ccgaatcgcg ggcattgcaa tcgtgcgtca aaaatttctg cttggcgatt
    16261 acttggagtt gctgggagtc gccgcctggg ccagaaatca gggagcaggg aagcagctca
    16321 tcggccacgt cgaatccttg gtgttcgcgc gaacgaagaa tctcttcgcc tgcgtatccg
    16381 acttcaacaa gggtgcccgg gaattttacc ggaagcaagg ctatcacgag atcggtccca
    16441 tgccgaactt cttgattcca ggtagtgcgg aaatccttct gcgaaaaacc gccggtccgg
    16501 caagagagca gagccgctag tcatcgatcc tgtgtcgttg ctaagattaa ttaaaactcg
    16561 ccgtccctaa acgggctgac aacgatggtc gactgatgac tgattttaaa gtgaccaaac
    16621 tccttcatac caggatgcgc gtgagcgaca tggatcaaac gatcgccttt tataccggcg
    16681 tgcttggcct tgaggtgctg gaacgaaaaa cgtcgcctcg aggctcccac ctggcctttc
    16741 ttcgagtacc ggacagtgaa gaattgattg agctctgcag tttccctccg agcggagccg
    16801 tgaaggttca accagatctc gtgcacctcg cctttcaggt ggagaatctg gaccgcacga
    16861 tgacagccct gcagcaaaag ggtatcccca tcacggatgg tccgaccacg acgtcttcgg
    16921 ggagccgttt cgtgttcatc gatgcgcctg acgggtatga ggtcgaattg atcgagagac
    16981 cagcgggcgc gaaaatcgtg taacgggcgg cgcgatccac ctcgacaccg gcatattcac
    17041 tttcgccatc tcaccactag gtgatcacac ctctggaacg attcatttcg cactcactat
    17101 tgacccatgc caattgacca agtacgctcc cgccctgtat ttctctcggt gcaatacccc
    17161 caggagctat gaaatgaaga acggattttt ccgcgggcac ggcttgggaa acgattatct
    17221 ggtgatggac ccgaaggagc tgagcttcaa gctcacgccg agcacgattg ccgcactctg
    17281 cgacaggaac tggggattag gcagcgacgg catcctggcc gctgcttcct cgaccaaggc
    17341 cgactttggt ctgcgcattt tcaacccgga cggcagcgaa gcggaaaaat ccggcaacgg
    17401 cctccgcatc ttcgcccgtt atctccatgc gaccggcaag acgaagaaga aacattttac
    17461 ggtggaga
//
LOCUS       DASTQY010000179         2929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_840589, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000179 DASTQY010000000
VERSION     DASTQY010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840589"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFR82_05915"
     CDS             <1..269
                     /locus_tag="VFR82_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU4501170.1"
                     /translation="KQYEKLLSFEKVKLKFTEGALGAVARKAFTQKTGARGLRAILEE
                     VMLDVMYDAPSQKQIKEVFITEDAILGKHPPIRIFEQDKDIRTA"
     gene            491..1012
                     /locus_tag="VFR82_05920"
     CDS             491..1012
                     /locus_tag="VFR82_05920"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HEU4501171.1"
                     /translation="MKYPVVSSLRHKGKTIELDSAREIITLYGINGDAMGNLSWDFVI
                     DQILAYRKPPASREARTEPRVTLAFRVKFKTPEGQQFESRAGGIGGGGLFIESQSPLA
                     VGTKLSLEFSLPERGNEWLAAKGVVAWVCPKADQYTFSPGMGVRFTDIAADIRNRVLE
                     LVKSLQEVGRPAA"
     gene            1079..1597
                     /locus_tag="VFR82_05925"
     CDS             1079..1597
                     /locus_tag="VFR82_05925"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4501172.1"
                     /translation="MKFPVIATAGHQGETLEIDPSREIISLHGANGALLGAVSWNDVI
                     ERIAAGDEDIRFAHARAHARAPLAVKVKYTTPEGKQFDSLTGGVGGGGLFIESSQPLA
                     PGTELSIEFALPDRPWEKHKAKAKVAWTRNKPERFLLFPGMGVQFTDIDEKSRHDLVE
                     LVAALNRSRATT"
     gene            complement(1572..2231)
                     /locus_tag="VFR82_05930"
     CDS             complement(1572..2231)
                     /locus_tag="VFR82_05930"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU4501173.1"
                     /translation="MTALLKDHNPEVRRTAVESLGKIGDRTAVPSVLPLLTDPIPLVR
                     GAAAQAMGRMATPADEVAVAGLTEALRDPADNVRQAAAMAIGDIEPSPRQLAVITTLA
                     QAADVRVRRTVVHALQSLDTSPVASALLPLADDSDAEVRQGTVACFGNSDDVRAGRVL
                     IQRLAQDPSPAVRAQAAYHLGEVRGIDARSVLQAAAAKERDSGVRRWIEAELRSLRGS
                     D"
     gene            complement(2355..>2929)
                     /locus_tag="VFR82_05935"
     CDS             complement(2355..>2929)
                     /locus_tag="VFR82_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501174.1"
                     /translation="TATTAQAAPKPTAPYTPGPELTPGTTAASLYNMGPQAPQPGRPT
                     ASPPGTQTARVAQPPTMEERAAAPTPMMRGQYPPLPPSAQQKPQAKRPSAIVNKDAVA
                     RVQAQQAALKAKATPTGGVSPADMQHLGEGLGDALRTFKQAVADVRNSVDPEMRTIQA
                     EMDAAQKEIQQSIEAAREMPAVEDEPPKPA"
BASE COUNT          599 a          851 c          883 g          596 t
ORIGIN      
        1 ccaaacagta cgaaaaattg ttatcgttcg agaaggtcaa gctcaagttt actgaagggg
       61 cgctgggcgc tgtcgctcga aaggccttta ctcaaaaaac gggggcacgg ggacttcggg
      121 ccattttgga agaagtcatg ctggatgtga tgtatgatgc gccgtcacaa aagcagatca
      181 aagaggtctt catcactgaa gacgccatcc tcggcaaaca cccgcccatc cgcatttttg
      241 aacaagacaa ggacataagg accgcctgat cccttatatt tcaatcgctt cagtcttacc
      301 ggctcggctt ctgtccgggc cggttttttt acgatactag taaccgattc gagcttttcc
      361 attcgggcca tctgagttcg gtgccatcac ctcaccctgc gcagaatccc gcctgcgaaa
      421 ctctcggctt ttactcgaca agatgaaagg ctgcgctata cttgccgagg gtgagcaaga
      481 gggaggatcc gtgaaatatc cggtggtttc cagcctgcgc cataagggca agacgatcga
      541 attggactct gccagggaga tcattacgct ctacgggatc aacggagacg ccatggggaa
      601 tctgtcgtgg gactttgtca ttgatcagat tctcgcctac cgtaaaccac ctgcatcccg
      661 agaggcgcgg acagagccgc gggtgacgct cgcatttcgt gtgaagttca aaacgccgga
      721 aggccagcaa ttcgagagcc gggcaggggg catcgggggc ggcgggctgt tcattgagag
      781 ccagtctcca ctcgccgttg gcaccaagtt gtcgctggaa ttttccctgc cggagcgtgg
      841 gaacgaatgg ctcgccgcta aaggggtggt cgcctgggtc tgtcccaagg cagatcaata
      901 caccttcagt ccgggaatgg gcgtccggtt caccgacatc gccgccgaca ttcgcaaccg
      961 cgtactcgaa ctcgtgaagt ccctgcagga agtcggtcgg cctgccgcct gactgcatga
     1021 atgactgcat gaacgcaagg gcattacatc acaacgtcct tcaagaaagc gtgacagcat
     1081 gaaatttccg gtcattgcga ctgcaggtca tcagggagag actttggaga tcgacccgag
     1141 ccgcgaaatc atttctcttc atggagcaaa tggcgccttg ctcggagcag tgtcatggaa
     1201 tgacgtcatc gaacgcattg cggcaggcga tgaagatatc cgctttgcgc atgcgcgcgc
     1261 gcatgcacgc gcccccttgg ccgtcaaagt gaaatacacc acgcctgaag gcaaacaatt
     1321 tgacagcttg accggcggcg tcggcggcgg aggcctgttc atcgaaagca gtcagccttt
     1381 ggcaccgggc actgaactgt ccatcgaatt tgcccttcca gatcgtccct gggaaaagca
     1441 caaagcgaaa gcgaaagtgg cctggacacg caataagccg gaacgctttc tgttgttccc
     1501 cggcatgggc gtccagttca ccgatatcga cgaaaaatcg cgccatgatt tggtcgaatt
     1561 ggtggccgcg ctcaatcgct cccgcgcaac gacctaagct ccgcttcgat ccacctccgc
     1621 acaccactat cccgttcttt tgctgcggca gcttgcaaga cagagcgagc gtcgattccg
     1681 cgcacttctc ccaagtgata ggccgcttga gcacggacgg cgggagatgg atcctgcgcg
     1741 agcctttgaa tcaatactcg gcccgcccgg acgtcgtcag aattgccgaa gcacgcgacg
     1801 gttccctgcc gcacctctgc gtccgaatcg tccgcgagtg gcagcagggc cgacgccaca
     1861 ggactcgtgt ccagggactg caaggcgtga acgacggtgc gcctcacccg tacgtctgca
     1921 gcctgcgcga gcgtggtgat gaccgcaagc tgccgaggcg agggttcaat gtcaccgatg
     1981 gccatcgcgg cggcttgccg gacattgtcc gccgggtctc gcagtgcttc agtgagtccg
     2041 gcgaccgcga cctcatccgc aggggtcgcc attcgaccca tggcctgcgc ggctgcgcca
     2101 cgtaccagcg gaatcggatc ggtcagcagg ggcagcacag aggggactgc ggttcgatcg
     2161 ccgattttac ccagcgactc gaccgccgtc cgccgcacct caggattgtg gtccttgagc
     2221 aatgcggtca agagtgtctt gctttggtcg gggagaggcc cgggtgcttc ttgaagacac
     2281 cccgccagcg tcagacagaa aagtaggaag agggaaagag gggagcggag tgacaagctc
     2341 cattcatcca tgcgtcaggc aggttttgga ggttcgtcct ccacggcagg catttctcga
     2401 gcggcctcga tagattgctg gatttccttc tgcgccgcgt ccatctcggc ttgaatggtc
     2461 cgcatctctg gatcaactga attccgcacg tctgccaccg cctgcttgaa cgtgcgcaag
     2521 gcgtcgccga gaccttctcc aagatgttgc atgtcggccg gcgacacgcc acccgttggt
     2581 gttgccttcg ccttcaaagc cgcctgctga gcctgcacgc gagccacggc atccttgttc
     2641 acaatcgccg acggtcgttt agcctgtggt ttctgctggg ccgagggcgg aagaggaggg
     2701 tactgaccac gcatcatggg tgtcggggcc gctgcccgct cttccatcgt tggcggctga
     2761 gcaactcgag cggtttgtgt cccgggtgga gaggccgtcg gccggcccgg ttggggcgcc
     2821 tgtggcccca tattatagag cgaagcggcc gtcgtgccag gtgtcaattc gggaccaggt
     2881 gtgtagggcg cagtcggctt aggagcggct tgagcagttg tagccgtgg
//
LOCUS       DASTQY010000180        11377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_160376, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000180 DASTQY010000000
VERSION     DASTQY010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11377
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160376"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..500
                     /locus_tag="VFR82_05940"
     CDS             147..500
                     /locus_tag="VFR82_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501175.1"
                     /translation="MDAYVIGFLLGVFMAGLSAVGLWAGLGLIVSVLRRLKKKESGMM
                     ILVYSVETIISLSVVIALGGLASRVSFDATSYWLGVSLTIVGNGFIFVAALGKMWNAL
                     KSELAAWQDRPRSKN"
     gene            657..1085
                     /locus_tag="VFR82_05945"
     CDS             657..1085
                     /locus_tag="VFR82_05945"
                     /inference="COORDINATES: protein motif:HMM:NF039957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6010 family protein"
                     /protein_id="HEU4501176.1"
                     /translation="MVPVVVAALLAVGIAALAKFTRFDEDRSFYSTVLVIIASYYVLF
                     AVLDGSGHALAWELVIAVAFSAVAIIGALVLPTLVGIGIIAHGLFDLVHDVFITNSGV
                     PTWWPSFCGSIDVLLGLWVFFLTRSRLTYRRASNLKRTLT"
     gene            1262..1594
                     /locus_tag="VFR82_05950"
     CDS             1262..1594
                     /locus_tag="VFR82_05950"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEU4501177.1"
                     /translation="MPFYLTRFSYTPETWAKLIKNPEDRRVAAQQYIESVGGKLHGFW
                     YAFGDHDAYNLWEAPDNVSMAATAIAIAAGGALSSLHTTPLLTVEETIAALQKASPIK
                     YRPPGDKR"
     gene            1813..2193
                     /locus_tag="VFR82_05955"
     CDS             1813..2193
                     /locus_tag="VFR82_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4501178.1"
                     /translation="MKNKVVMFEIPASDFKKAKEFYETVFDWKVDLWGDEGAMALTTA
                     ADKDFNPTEPGGINGGFYKRKSKADQPSCGVETESIDETIKAIEKAGGKVVTPKHPID
                     EWGFMADFADPDGNVLSLWERPKQ"
     gene            2592..2984
                     /locus_tag="VFR82_05960"
     CDS             2592..2984
                     /locus_tag="VFR82_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501179.1"
                     /translation="MPETGPFYNMPVSHSASDRKAALAVAKEMKADFVIFVEHEESKE
                     GALIEPQCGSLFHVNVTVRGLLVETGDTAFRGSAHYPHCVDLSGETFRNLTCQAAATA
                     WGFRPSGQLDIPSSLMCTAGQTEPTPIR"
     gene            3242..3949
                     /locus_tag="VFR82_05965"
     CDS             3242..3949
                     /locus_tag="VFR82_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501180.1"
                     /translation="MRFALVVFSLLCVFSFAEAKEYGNYNLKHSLKKIITVSDASTGA
                     HTSVHLDYLDQILTDLSLHARNYPPTFDTPQDKARAVEDVKTLSVLLDILVNGPNPHP
                     ELLLRAGLLNSIGHNLDIPGTADEAKLLFQRLLATSPSDPRANYHYGTFLAGAAKSRE
                     ALPYLEKALSAGVGDAAYSIGMAYLILGDKQKALANLEAYQQHRPNDEPLAKLIDDIR
                     NGQFKIQRSRMRDDRAR"
     gene            complement(4037..4435)
                     /locus_tag="VFR82_05970"
     CDS             complement(4037..4435)
                     /locus_tag="VFR82_05970"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU4501181.1"
                     /translation="MKTSGSQNIKSPSQLIDARIQELGDWRGKMLSRLRTLVKEADPH
                     VVEEWKWRGVPVWSHDGIICTGETYKNVVKMTFAKGAALKDPSGLFNSSLDGNTRRAI
                     DFHEGEKIDEEALRTLIRAAVTLNKSKAKS"
     gene            4632..6062
                     /locus_tag="VFR82_05975"
     CDS             4632..6062
                     /locus_tag="VFR82_05975"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HEU4501182.1"
                     /translation="MKSAIERELKLTVAAGFKLPPLKGARLPAKLLTSVYFDTPDLRL
                     ARARITLRHRTQGRKSAWQLKLPLDCGRREVEMPGKSSIPPPQILDLLFIHLQDGAVQ
                     PVATLRTRRTGIRVGGSKGADVVLDEVAILKESSEVLIFREVEIEQLDGDASLANRLE
                     KTLRKAGATDHDGRPKIFRALDLPAPQRPAPPDSDAHASAHLRYILGRQLNTMLSRDP
                     GVRLGGDSEDVHQMRVATRRMRSVLRVARPLLKSGWEEPLRDKLGWLGRQLGEARDLD
                     VQIAYFKGQSDSVKPRDRAALERFLEYLQKKRNKVQEQLMSHLRRRRYVTLINRLIPA
                     VHDPAMVPNVDVTLPDLARKAFRKVKQAVKELDDSASNAKWHCVRIRTKRARYAAELS
                     ESCSGRAATRFIDKIKLIQDQLGDIQDAVIAEDHLRRFTSRTSRHSALLAGQMVERQR
                     QRRRHAKQAFYSTWKQVKKCGKEAWC"
     gene            6567..7802
                     /locus_tag="VFR82_05980"
     CDS             6567..7802
                     /locus_tag="VFR82_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4501183.1"
                     /translation="MPTLTRTGEVKDVVITEGVEPTSVTVNPGDEIRWINKRQGVVRV
                     IFLSPMTENLTCQRHFGGRMGFGTKRNEYSARLRPNKTASVCFREAGEVRYVVRAESS
                     DSSGEQNLAGTISIGPEYQARRPGVEPVNRNAGLAAHSDKNEAAAIMSSRLVAADRDR
                     QRLAEELAIAQRQLAERDASLASLHSQLDQRLAAQAGKDEAYSDMSSRLAAADQDRQR
                     LAEELAVAQGQLADRDASLASLSSQLEQNKSNLAHAEKDILKALQPEISNGTVMVQQS
                     GQALTINLASSLLFDSGQDHMKAGATDALKRVGTVLKDFPDKQVHVAGYTDNVAITGS
                     LQKKFPSNKELSDARANSAAQALRDGGLSVNLEAAGYGDSNPIATNTTATGRAKNRRV
                     EIIVASSGNQAVAKTPDLP"
     gene            8185..8754
                     /locus_tag="VFR82_05985"
     CDS             8185..8754
                     /locus_tag="VFR82_05985"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HEU4501184.1"
                     /translation="MSMMRQSHNCMLMLGLPVLVGITIVASSIRAGAASDSNPAAHSS
                     EQKWDKKLPSASRFTVLSTFEDAAVRDDETGLVWEKTIETTEMSWTDARAVCADKNVG
                     GRKGWRLPSISELTSLVDPSTRSGPTLPTGHPFTNVLMDVYWSATSVTDNPKNAWLVF
                     FDTGKATFAFKTITFHVWCVRGTIPAGQY"
     gene            complement(8814..9269)
                     /locus_tag="VFR82_05990"
     CDS             complement(8814..9269)
                     /locus_tag="VFR82_05990"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEU4501185.1"
                     /translation="MVVWRLTLRKHSVPDGEGARRYGGRWNKPGTPVVYTSGTLSLAA
                     LEYLVHVDSDILPDGLVSIRATIPDGLKMEAINASHLPLEWKDKIIPVSVQQLGTDWV
                     TNAKSAVLKVPSVVIEHEWNYVLNPRHPEFSRITWDAAAVFSFDQRLLT"
     gene            complement(10205..11083)
                     /locus_tag="VFR82_05995"
     CDS             complement(10205..11083)
                     /locus_tag="VFR82_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501186.1"
                     /translation="MISIKQTLLTALSKRLAIMCVLLLGFPTLAISASDEQTVGELME
                     LSGLTQQIPRLPNDYMTLVDQLLVGLERQRHPVSKTLRRQIRQSFVEALTTENFESEI
                     RSRLLENLSQDTALTTTSWLRSDLGQKITTAEVNASSAEKSVQFATFFLQLQLERPAP
                     ERLQVVRRIEAITQGSEMATEAWEAIVAAIGRALEGEYRANPQSKTNLEEYLASMRGS
                     VKGMFEQGRLFQALFTYRTLTDEELKEYAEFLESPAGKDVTHTINRAVQGAAIDAIQN
                     IKPSRTKSIQPPRETA"
BASE COUNT         2656 a         3091 c         3044 g         2586 t
ORIGIN      
        1 ggaacgctcc taaaggagag cgatggctca agcgcaaaga catgccggct aggaagggat
       61 atcgatgaag tagggtccaa acgattcgtt atccggattc gaacagctac cgggagcagc
      121 tgtcactcgt cagcatagga ctattcatgg acgcatatgt cattggattc ttgttaggtg
      181 tgttcatggc gggccttagc gctgtcggtc tctgggcagg tctcgggctg atcgtttccg
      241 tgctccgaag gctcaagaaa aaagagagtg ggatgatgat tctcgtgtat tcggtcgaga
      301 cgatcatttc actaagcgtg gtcattgcgc taggtgggct tgcgagcaga gtgtcctttg
      361 atgccacttc ctattggctg ggtgtgagcc tgaccatcgt gggaaatgga ttcatatttg
      421 ttgccgcgtt gggcaagatg tggaatgcgc tcaaaagtga actggcagca tggcaggatc
      481 gaccacgatc gaaaaactga ttgccgagag ccaacaacat tctgaattgt tcgggtcgtg
      541 gcagtcgcat tccgcagtgc cctcgtcctt acgctgtttg ttctcactcg cgatagacgt
      601 gcccgtacca acatcgtaaa ataggccggc tcctcattcg cacggcgagg tcttttttgg
      661 ttcctgttgt tgttgccgct ctccttgctg ttgggatcgc agcactggcc aagttcacac
      721 gcttcgatga agaccgcagc ttctattcta ctgttctcgt catcatcgca tcatattatg
      781 tgctgttcgc agttttagac ggttcaggtc acgccttggc atgggaactg gtcatcgccg
      841 tcgcgttttc ggcagtagcg atcatcggcg ctctcgttct tcctacgcta gtgggaattg
      901 gaatcatcgc ccacggtctc tttgatcttg tgcatgatgt tttcattacg aattctggcg
      961 tcccgacctg gtggccaagc ttctgcggga gcattgatgt cttgctgggg ctgtgggtgt
     1021 tcttcctcac ccgttctcga ctcacatata gacgcgctag caatttaaaa agaaccctca
     1081 cataaaagaa caaagggaga ttctgttttc ccggcgcgtg gcgagctttt taaacagaaa
     1141 gcgcagtaca ccgtcggcat atgtgagagg ttgcgacgcc caacgcaacg gagtaatgtt
     1201 cccaccgcaa ccaaaggtgg ccgctcgata ctggaacacg cccaaaaagg aagaaagacc
     1261 aatgccattt tatctgaccc ggttcagtta cacaccagaa acgtgggcga agctcatcaa
     1321 aaatccggag gaccgccgag tggcagctca gcagtacatc gaatcagtcg gtgggaagtt
     1381 gcacggtttc tggtatgcct tcggcgatca cgacgcctat aacttgtggg aagcacccga
     1441 taacgtctca atggcagcca cggcgatcgc aattgccgcg ggaggagcgc tgagttccct
     1501 ccatacaacc ccgttactaa cggtcgagga gacaatcgcg gctctacaaa aggcttcccc
     1561 gatcaaatat cggccaccgg gtgacaaaag atagtgaaac gacacccgcg gcatggacac
     1621 gccggccgta cgttcgaata atccctccgc ctcgaccacg ccagggccgc tctaatttca
     1681 aggcgtttca ctagttccgt cacttttatt gacgtaccag actggaggct tattacgcgc
     1741 aaagggaata tccctccgga acgcctgtcg ctgatgccag atagccactt gatgtgatgg
     1801 aaggaggaaa tgatgaaaaa caaagtcgtg atgttcgaga ttccagcgtc tgattttaag
     1861 aaggccaaag agttttacga gacggtgttc gattggaaag tggacttgtg gggcgatgaa
     1921 ggcgccatgg cgctcaccac agctgcggat aaagacttca atccgactga gccgggcggg
     1981 atcaacggcg ggttctataa acgcaaatcc aaagccgatc agccgtcgtg tggagtggaa
     2041 acggaatcga tcgacgagac gatcaaagcg atagagaaag cgggcggaaa ggtggtgacg
     2101 ccgaaacatc ccatcgatga gtgggggttt atggctgact ttgccgatcc tgacggcaac
     2161 gtcctatcgc tgtgggaaag gcccaaacaa taaagctccg tgttgggacg gcagcagaga
     2221 tgaacccgcc acctgcattt cgcgggtggc gccggcccgc tgaaagaacg aggaccctgg
     2281 gtcactagac agcgcattct gggtcctgac agcagcactg gttagaacgc gaagcgagga
     2341 ctacaatcct cgtcatgcgc tttctgcacc atcgcgcccg tctgcttgaa aggctttgcc
     2401 ttctctccct tgtacttctg gcacagggct gcccttctaa agcgccaacg ttgggcgtcc
     2461 gattcccgat cacacacggt tctcatacca ttttgccagc tgctcagcag cgaatccttc
     2521 tctgggggga tgctgccctg accgacgtcg cgttggaatg gctccggtcc catcattatt
     2581 cgagtatcct gatgcctgag acaggcccgt tttacaacat gccggtttca cacagtgcct
     2641 cggacaggaa ggcggcactg gctgtcgcca aagagatgaa ggccgatttc gtaatatttg
     2701 tggaacacga ggaaagtaag gaaggggcgc tgattgagcc gcaatgcggc tctctctttc
     2761 atgtgaacgt gactgtccga ggcctgttgg tggaaaccgg agatacagct ttccgaggca
     2821 gcgcccatta cccgcattgt gtcgatctca gcggcgagac atttcggaac ctgacttgcc
     2881 aggctgccgc caccgcatgg ggatttcggc catccgggca actagatatt ccatccagtc
     2941 tgatgtgcac agcagggcag accgagccga caccgattcg ctagcagtta gcgagtatcc
     3001 gcaactgacg agtatttcct tctaagcctg ctctaatggc ctcttgatct ttcgcgagta
     3061 tgtcggaacg ccgattcatt acattgattg aatcttcctc tcctccctga cgaccttctt
     3121 ctgcaggtgg attggccaga cttatcgacg agccttgtcg cctgtcctgc ggcaattcct
     3181 ccgcacccac atcagttggg taccaaacca ctccgggttc gagtgaagat agccgacagt
     3241 tatgcgattc gctttggtgg tcttctcatt gctttgcgtc ttttcttttg cagaagcgaa
     3301 agagtacggg aattacaatc tcaagcacag tcttaaaaag ataattacag tttctgatgc
     3361 ctccactgga gcacacacaa gcgtacatct agattacctc gaccaaatcc tcaccgacct
     3421 tagtttacat gcgaggaact accctcctac attcgatacc cctcaagaca aagcacgcgc
     3481 cgtcgaggat gtaaaaacat tgtccgtgtt gctggatatt cttgtgaatg ggccaaaccc
     3541 gcatcctgaa ctgctattgc gggcaggttt gcttaacagc attgggcata acctcgatat
     3601 ccctggcaca gccgatgagg ccaagctact tttccaaagg ctgttagcta cctctccatc
     3661 agacccgcgt gcgaactacc attatgggac ttttctggct ggggcggcta aatcaaggga
     3721 ggctctacct tatttagaga aggccctttc agcaggtgta ggagatgctg cctatagcat
     3781 tggtatggca tacctgattc tgggcgacaa acaaaaagcc ttggcgaacc tggaagccta
     3841 tcaacaacac cgtccaaatg atgagccttt ggccaagctt attgacgaca ttcggaatgg
     3901 acagttcaaa atacagcggt cacgcatgag agatgaccgc gctcggtaat gagttcgaga
     3961 aaatacatcg aagatctgac cattaaggat gtcaggactt atttccggcg tgcatgcgga
     4021 tcgtttgatc cgtggattag cttttggctt tggacttgtt cagggtcacg gcggcgcgaa
     4081 tgagagtcct cagcgcctcc tcgtctatct tctcgccctc gtggaagtcg atcgcgcgcc
     4141 gtgtgttccc gtccaaactg gagttgaaga ggcccgaagg atctttcaaa gccgcccctt
     4201 tagcgaaagt catcttcacg acattcttgt aagtctcgcc cgtgcagatg attccgtcgt
     4261 gcgaccacac cggaacgcct ctccatttcc actcctcgac aacatgggga tcggcctctt
     4321 tgactagcgt acggagccgg ctgagcatct tgcctcgcca gtcgcccagt tcctgaattc
     4381 tcgcgtctat gagctgagat ggcgacttaa tgttttgaga cccgctcgtt ttcatgcccg
     4441 tttctcctca ttacaaaaca aatggatgct gtgtacaaaa gttcgtcatc tcttagaact
     4501 aggtaaacgg tatataccag aacccctgag atcgtggagt aattccctga tgagctatag
     4561 aagcgttcaa ttcccccatc agatagtgtt acattcgaca aatgggaaat gcgaatcgac
     4621 aagtgtcttc cgtgaagtct gccatcgagc gtgaactcaa gctcacggtt gctgcgggct
     4681 tcaagctgcc tcccctcaag ggtgcacgtc ttcctgcgaa acttctcacc tctgtctact
     4741 ttgatacgcc cgatctgcgg ctcgctcgtg cccggataac gctccgtcac cgcacacaag
     4801 gaagaaagag cgcctggcag ctcaaactcc cgctcgactg cggccgtcgc gaagtcgaaa
     4861 tgccggggaa atcctccata ccaccgcctc agattctgga tctccttttc attcatctgc
     4921 aagatggagc cgtgcaaccg gtcgccacgt tgcgcacccg gcgaacaggc atccgcgttg
     4981 gaggatccaa aggcgctgac gtggtgttgg acgaagtggc catccttaag gaatcatcgg
     5041 aagttcttat ttttcgcgaa gtggaaatcg aacaactcga cggcgatgcc tcgctggcta
     5101 acaggctgga aaaaacattg cgcaaggccg gcgcaacgga tcacgatggc cggccaaaaa
     5161 tcttccgggc gctggatctg cctgctcctc aacggccggc gcctcccgat agcgacgccc
     5221 acgcaagcgc tcatcttcga tacattctcg gtcgtcagct gaacaccatg ttgagccggg
     5281 accccggcgt gcgtctgggc ggagatagcg aggacgtgca tcagatgcgc gtggccacgc
     5341 gccgtatgag gtccgtcctc cgagtggccc ggccgctgct gaaatcagga tgggaggaac
     5401 cgctgcggga caagctcgga tggttaggcc gccaattggg ggaagcccgc gatctggatg
     5461 tgcagatcgc ctatttcaag ggccagtccg acagcgttaa accgcgggac cgtgcagcgc
     5521 tcgaacggtt cctcgaatat ttgcaaaaga agcggaataa ggtccaagag cagcttatga
     5581 gccatcttcg gcgccgtcgt tatgtgacgc tgatcaaccg gcttattcct gcggtccacg
     5641 accccgcgat ggtgccaaac gttgacgtca cactccccga tctcgcccga aaggcgttcc
     5701 ggaaggtgaa gcaagccgtc aaagagctcg acgattccgc ctctaatgcc aagtggcatt
     5761 gtgtgcgcat ccggacaaaa cgggctcgtt atgctgccga attgtcggag tcgtgttccg
     5821 gccgggcagc gacacggttc atcgataaaa tcaaactcat tcaagaccag ttgggcgata
     5881 ttcaggatgc ggttattgcg gaggaccatc tccggcgatt cacatcaaga acatcaagac
     5941 attcagcgtt gctggcaggt cagatggtcg agcggcaacg ccaacgccgg cgtcacgcca
     6001 agcaggcctt ctactcgacc tggaaacagg tcaagaaatg cgggaaagag gcatggtgct
     6061 gatgccctag cctaagttcg ccgcctcagg atcgattcca acactgctca ccgccgcgta
     6121 cggcttcgtc acggaaacct tcatcagatg aatcagttgt cgtgaaccat ttcgtacccg
     6181 ctgacttaca atcaccaagt cagcacccag tcctgcctga cttttcttat catcctaact
     6241 aggttcatat aagccaaacc ttcgccttgc aattggcgtt gtcgcacccg cacatgggtg
     6301 tggtcaatct tatacagact gcttctcgat aggcatttca taatctccgc aaacatttgt
     6361 ccgaagctga gatcaggcaa cagcgcctgc caggcgatgg ctccatttgc aacggcctcg
     6421 attcagccgc ctatgcctca atcagcgcct gctcagtctt gaaacacata caacgataag
     6481 gaggcaatac aatgtatgcc caacgcatca tccttccatc cgctctgctc atactggctg
     6541 gctgtcaaac catcggcgga gagggcatgc cgaccttgac ccgtacgggt gaagttaaag
     6601 atgtggttat cactgagggc gtcgagccga catccgtgac agtcaatccc ggcgatgaga
     6661 tccgctggat caacaaacgt cagggcgttg tccgggtgat ttttttgagt cccatgacag
     6721 aaaacctcac ttgccaacgc cacttcggcg ggaggatggg attcggcacc aagcgcaatg
     6781 agtactcggc aagactgcgc cccaacaaga cagcaagtgt gtgtttccgg gaagccggcg
     6841 aagtgaggta tgtggtcagg gccgaatcaa gtgactccag cggagaacag aatctagcgg
     6901 gcacgatttc aatcggtccc gaataccagg cgcgacgacc gggggtcgag ccggtgaaca
     6961 ggaacgcagg ccttgcagcc cactctgata agaacgaggc ggcggcaatc atgagttcgc
     7021 ggctggtggc ggcagaccgg gaccgccagc ggctggctga ggaactggcg atagcccaaa
     7081 ggcaactggc agagcgcgat gccagcctgg ccagcctgca cagccagctt gatcaacgcc
     7141 ttgctgccca ggctggtaag gacgaggcct actcagacat gagctcgcgc ctagccgccg
     7201 cagaccaaga ccgccagcgg ctggctgagg aactggcggt agctcaaggg caacttgcag
     7261 atcgcgatgc cagtctggcc agtctgagca gtcagcttga acagaacaag agcaatctcg
     7321 cacacgctga gaaagacatc ctgaaggcgc tacaacctga aatttccaat ggcacggtga
     7381 tggtgcaaca atccggtcag gcgctcacga tcaatttggc ctccagcctg ctgttcgatt
     7441 cgggccaaga ccacatgaag gcaggtgcca ccgatgccct gaagcgagta ggcacggtcc
     7501 tcaaggattt tccggacaaa caggtccatg tggcgggcta tacggacaat gttgcaatta
     7561 cgggcagcct gcaaaagaag ttcccctcca ataaggaact gtcggatgcg cgagcaaaca
     7621 gtgcagcgca ggcgctgcgg gatggcggcc tgagtgtcaa tcttgaggcc gcaggctatg
     7681 gggacagcaa cccgatcgcc accaacacca ctgccacggg gcgcgccaag aatcgacgcg
     7741 ttgaaattat cgtagcctct agtgggaacc aagctgttgc caaaacacct gacttaccgt
     7801 gatttaagtt cagagtctta tatgtgaaag aggcccggcc ggggacgcac accggccggg
     7861 ttaagcgctc accacgcgcc cgttccatac cggtcgccgt tttgtacgac aatcctcacc
     7921 atcgcttttc gcgttacgaa tcagatgcca agttggtcgc gatcgggcgt catgttcacc
     7981 gtcgccggac aaaacgacat ccagccatct atccatacat gaagatgaat ctttaatatt
     8041 ttctcgagca caactcacag agatcccggt ttcactttcc ccatagaccc agcccatctg
     8101 aatacgtaga atgttgccgc tttctttcct attggtaccc gaaggcagtg ctggctgtga
     8161 ggtcatcgca gaaggaggga cgaaatgagc atgatgcggc agtctcacaa ttgcatgctg
     8221 atgctggggc ttcctgtcct cgtcggaatc acaattgtgg caagcagtat ccgtgctggc
     8281 gccgcatcgg attccaatcc agccgcgcat tcatccgaac agaagtggga caagaagtta
     8341 ccgagcgctt cgcgttttac tgtcttgagt accttcgaag atgcggcagt tcgagacgat
     8401 gaaacggggc tcgtgtggga aaagaccata gaaacgaccg agatgtcctg gaccgatgct
     8461 cgtgccgtct gcgctgataa gaacgtaggc ggacggaaag gctggaggtt gccgtcgatt
     8521 tcagaactga caagtttggt ggatccctcc acacgctcag gacccaccct acccaccggc
     8581 catcctttta ctaacgtact gatggacgtc tactggtcgg cgacatcggt aacggacaat
     8641 ccgaaaaacg cgtggctcgt gttcttcgat acgggaaaag ccaccttcgc ctttaagacc
     8701 attacctttc acgtctggtg tgttcgcggg acgatacctg ctggccagta ctgaagttag
     8761 gtgtgagaaa gagtgagaac catttcgcga cgcgtgtcgg gccggctcag gttttatgtc
     8821 aacagacgct gatcgaaaga gaacacggca gcggcgtccc atgtgatccg ggaaaactca
     8881 ggatggcgag ggttaagaac ataattccat tcatgctcga tcacgacaga tggaactttt
     8941 aagacagcac ttttggcgtt ggtaacccaa tcagtaccaa gttgctgcac acttacgggt
     9001 attattttat ctttccattc gagtggcaag tgagaggcat tgattgcttc catcttaaga
     9061 ccgtcgggaa ttgtcgccct tatactgacc aatccatcgg gaaggatatc cgaatcaaca
     9121 tggacgagat attccagagc agcaagagaa agggtgcccg aggtgtagac tactggcgtt
     9181 ccaggtttat tccatcgtcc tccataccgt ctcgctccct ctccgtcagg gacgctatgt
     9241 ttcctcagcg taagtcgcca aactaccact agcctatgac accgtgttcg agccgtccca
     9301 acatcgcctc aacggcttga gtgccaaggt cggtatcaag gagttctatt ggagctgttc
     9361 cttctaggat tcggtgtggc cgagtgagcc acgttgacgc tttttcttcg gtaccaaaga
     9421 cattcgacgc gtatgtcaaa attctggcta cgcgtgctaa gcgatcagat tcctgtgccg
     9481 ttaggcgctg ttctgctttt cgcctggcca tcgtacgagg tggcacgctg agaattctgc
     9541 tcattctggg caaatctaga tgaaatttag agaggacaaa ttccagcgat cgatagggaa
     9601 agcctttatg aatgatcgca cgaacatctt cagccgattg aacttgtcgc ttaaatatag
     9661 tggcccctcc taacgtctta aatagctctt cagcttgagg cataattccc tttcattgtc
     9721 atatggctaa atgttaatgc catatggcac aattgtcaat accgtatcaa ggacaaggga
     9781 gcgatacggt tgcagtcaac cacgcatcct gaccaacgtt atcagcccca tgatcagttg
     9841 ccccttttct ccgtggcact ttcggtccca aggcattaat atatgcggtc ttaaacgata
     9901 atcgttaaag ttttggttca ctctcttgct ggcgacaatc atgatctaca cattatcatg
     9961 caaccgtcgt cgggcgggac gtcggaaaag cgacagcttt cttttttttg ggcggaacgt
    10021 atcacacatc gcgcgtgcgc aagggaagtg cggttacagc cttctccttg cttggcacaa
    10081 cggaatggcc aggaattcga acccccagat ctaggcgaac cgtcgacgtt gatcgcggcc
    10141 taccctgcct ccgcgtggcg accccgctca ttgatcgtcg ggctatggaa aggagggact
    10201 ggagttatgc ggtctcgcgc ggcggttgta tgcttttggt gcgagaaggc ttgatattct
    10261 ggatggcatc gatcgccgca ccttgaaccg ccctattgat ggtatgggtc acatctttgc
    10321 cggctgggct ctccagaaat tccgcatact ccttcaactc ttcatccgtc agcgttcggt
    10381 aggtgaacag cgcttggaat aatcgtccct gctcaaacat gcccttgacg ctgcctcgca
    10441 tggaagccaa atattcttct agattcgttt tactctgcgg attagcccgg tactcgcctt
    10501 cgagagcgcg accaatagcg gccacaatgg cctcccaagc ttcggtcgcc atttcgctac
    10561 cttgagtgat cgcctcaatg cggcgcacga cttggagtcg ctccggcgca ggacgctcca
    10621 attgtaattg caaaaagaag gtcgcgaatt ggacggactt ttccgccgaa ctggcgttga
    10681 cttcggcggt ggttatcttt tgacccaaat ccgatcgcag ccagctcgtc gtcgtaagag
    10741 ccgtatcctg agagagattc tcaagcagcc gtgaccgaat ctctgattca aagttctcgg
    10801 ttgttaatgc ctccacgaag ctctggcgaa tctggcgtcg cagagttttt gaaaccgggt
    10861 gacgttgtcg ctctaacccc accagcagct gatcaacgag ggtcatatag tcattcggca
    10921 gacgtggaat ctgctgcgta agaccggaca actccatcag ctcccctacg gtctgctcat
    10981 ctgaggctga aatggctaag gtcgggaaac cgagcagcag aacacacatt atggccagcc
    11041 gcttggacaa cgctgttagc agtgtttgtt tgatggagat catggtcact cagaccatct
    11101 tagtgttgtg caacgatagg gaatagagcg tggtgagaac aattccccga aagggtgagg
    11161 caaagaggac gcgcccgtga gatcgttccc gtggttataa ccaaacccgg tgacaactat
    11221 cggtggccca ggcgatcacc actcaccagt gatgggccca gtccggagcc ggaatgggct
    11281 tcggttcgag tagagccaag tcttcgtcaa acacggtgtg ttcacgctcc gagccaagat
    11341 gacgtacctg gagcctgccg gttcgtgtcg ccacaag
//
LOCUS       DASTQY010000181         8028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_405785, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000181 DASTQY010000000
VERSION     DASTQY010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405785"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VFR82_06000"
     CDS             <1..1164
                     /locus_tag="VFR82_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4501187.1"
                     /translation="STQYVYSGSYFQPITKWWSQRLTLARATEIFRTSSGTIERDLVT
                     ATETVPFPLNSEINATSNRLEWQHDFQIAKPLAVTAGYQFREQLGENLDTLAGTTTLP
                     SKIISSNAGFAEAKLNLWDRLFGTAGIRQDEYNVFGSATTYRATAGYLLKETGTKLRG
                     SYGTGFRAPTINQLFFPDFGNANLKPEKSRSMDVALDQSLFKDRVFLSVGYFWTKYQN
                     LILAVFDPIGCNTLFGFCAQNIGEARAQGIEASAKVQLTRDQPWIKSLDLQLQYTQTS
                     TRNLTDDVRTRLPRWPLNQWSAILSYQPIEALRANLEARFVGQRFNDTNNEDSLPHFY
                     VWNASFSYDLNKTVQIYGRADNIFNRKYEEVLFFGTPVRSVFGGVRVNFDMPI"
     gene            1455..2048
                     /locus_tag="VFR82_06005"
     CDS             1455..2048
                     /locus_tag="VFR82_06005"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEU4501188.1"
                     /translation="MRITKVYTRTGDGGSTRLAGGQQVSKDCLRVEAYGTVDELSASV
                     GLARAMNEEASQVVGAAKRLERDLRWVQNKLFDVGALLATAPGQTFKNMPTIRSQDTV
                     KLERMMDRCQKDLAPLKEFILPGGGKVSATLHQARTICRRAERICVSLAKDEPLDAEI
                     IKFLNRLSDALFVLARWVAKTQGEPEFLWERNPDEKD"
     gene            2053..3564
                     /locus_tag="VFR82_06010"
     CDS             2053..3564
                     /locus_tag="VFR82_06010"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEU4501189.1"
                     /translation="MNARTLMIQGTASHVGKSVMTAALCRIFVRRGLRVAPFKAQNMS
                     NNSFVTPDGKEIGRAQAVQAAACRLAPQADFNPILIKPESHTAAQLVVGGEVAGSLCA
                     KDFGAVRRQYWTTVQEAFGRLSHTFDVVVLEGAGSPAEINLRHCDVVNMQMARHAGAP
                     VVLVGDIDRGGVFASLVGTWALLDEADRRHLKAFAVNKFRGDASLLETGVVRVTHETG
                     MSCAGVVPHWGDLEVPEEDSLGWDGWHGRTSKRADRLVIGIVDVPGISNFTDFDPLAM
                     EPDVELARLTESTDRPLDAVIFPGTKHTVQALGFVRQRDFDRLARRVLAEGGTVGGIC
                     GGFQLLGKVIRDPLHLESEHGHIEGLGMLPVDTTFAFPKIVEQVTGQHVESGCTVVGY
                     EVRMGRTVVALEARPFLEFQDGRRGTTTREGICAHDGRLFGTYLHGLFDQAPFRRWWL
                     NRLRTSKGWTGLPETHGLSLDARLDRLADFVEQHLSVSIIDRLLEEGVSYGTP"
     gene            3551..4096
                     /locus_tag="VFR82_06015"
     CDS             3551..4096
                     /locus_tag="VFR82_06015"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF014351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEU4501190.1"
                     /translation="MAPRKTESHLVFVVGGASSGKSEVALQLACKGISKNAPRAFVAT
                     GEGLDEEMTMKIARHRQSRSSDWNTVEVPLELAAWSKRYGQGYRTIVIDCLTMWLSNR
                     WRQGSKEQEILQDGRTFLTALRGLEARTVIVSNELGMGLVPGEADSRRFREVAGALNR
                     LVAKEADEAYFVVSGIPLRLK"
     gene            4106..5161
                     /gene="cobT"
                     /locus_tag="VFR82_06020"
     CDS             4106..5161
                     /gene="cobT"
                     /locus_tag="VFR82_06020"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEU4501191.1"
                     /translation="MRLTEVLNQICDVNAEVAARTQRRLDRLTKPQGSLGRLEQLAVQ
                     YCAITCEDKPAVPRGRVYTFAADHGVVDEGVSAYPRDVTAQMVLNFLRGGAGVNVLAR
                     HAGVEVRVVDIGVAHDFGPIAGLMNRKLIRGTRNMVHGEAMDRRTAEEAIEIGIELAS
                     EAVEEGVTLIGTGEMGIGNTTASAAIAAVMTGASVVQVTGLGTGIDELGRTRKISVIE
                     RALARHAPNADDPVDVVAKIGGLEIAGMAGLMLGAAAARIPVVLDGFIAGAAALIACG
                     LQPRCVQYMIASHRSVERGHRLVLEKLGLEPLFDLDLRLGEGTGACLGMSLVQAAVKV
                     LTEMATFSEAGVSERNQ"
     gene            5158..5922
                     /locus_tag="VFR82_06025"
     CDS             5158..5922
                     /locus_tag="VFR82_06025"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEU4501192.1"
                     /translation="MTTSLKPFIFAWQFLTAVPLSRDLHHPAPHELAGSMLWYPFVGI
                     LLGAFMVMTDMLLARWLSPEVVAALLILLLVVMTRGLHQDGLADTLDGLAGGRMPTDR
                     LTIMRDPRIGAIGATGLFVSLILRYSALVSLPEPLKFPALLCMPAIGRWAMVVGAYGA
                     SYARAEGGLAAPFLAHLSARHVLGATVVVVSLMAWSLGFAAALIVLLIGGILSRLITM
                     CCRYYFAGITGDTLGATNEVVEISFLLFMPFLTYLP"
     gene            5919..6872
                     /gene="cbiB"
                     /locus_tag="VFR82_06030"
     CDS             5919..6872
                     /gene="cbiB"
                     /locus_tag="VFR82_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HEU4501193.1"
                     /translation="MTPIDFLAACMVDAVIGDPPAWPHPVRWMGRLITLVEKWVRRCC
                     VGSGSLRLTGVALALGLPSLAFCAGWALMATGTAVHDWIGRGIGIWLASTVLAWRDLV
                     DHVRAVSRALKAGTLDGARHSVGLIVGRDTDRLSEPEIVRATVETIAESASDGIIAPV
                     FYLVVGGPPLALAYKAVSTLDSMIGHQDTAYRDFGWASAKLDDVVNWIPARLTGWLIA
                     LAAGAVCWNTRSVHRSGALLLRDGHKHPSPNSGRPEAAMAGALGIQLGGTNFYAGLRH
                     DRPLLGDAKEPLLAAHIDRAQQIMTAAYLIAIVACLGILWR"
     gene            6899..7969
                     /gene="cobD"
                     /locus_tag="VFR82_06035"
     CDS             6899..7969
                     /gene="cobD"
                     /locus_tag="VFR82_06035"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HEU4501194.1"
                     /translation="MHAVARDLRRPIGSILDFSASINPLGPSPQVKRALVAAAPLIHH
                     YPDPDCFNLKQVMARHWKISPDCLVIGNGSSELIDVIPRALGIRSALILGPTYGEYAR
                     AVDRSGGRYQMVLASRNEVYRPPLEQVTQRLQGQWSGPPVAIDAVFICHPNSPTGRPC
                     DLQQLEDLFQAAERGRVWVVLDESFIDYCDALTCVPRLQTYPRLIILRSFTKFYGMPG
                     LRIGYSMSSRSIAAILKEHQPPWSVNTVAQQAAEAAIADQRHARRCLAYVSRERARMT
                     KQLAALAGVTVIPSSANFVFAELPPSCGAGLITAALRRRGMLIRDCSSVEGCGPRSIR
                     MAVRTKVDNDRLLAALAEVLER"
BASE COUNT         1606 a         2161 c         2510 g         1751 t
ORIGIN      
        1 agtacacaat acgtctacag cggcagctat tttcagccga tcacgaagtg gtggtcgcag
       61 agactgaccc tggcgcgtgc gaccgaaatt ttccggacga gctcagggac aattgaacgt
      121 gatcttgtga cagcgactga aaccgttccc ttcccgctga actcagagat caatgcgacc
      181 agcaatcggc tcgaatggca gcacgacttc caaattgcga agccgttggc ggtgacggca
      241 ggctaccagt tccgtgaaca gcttggcgaa aatctcgata cactcgccgg tacgaccacg
      301 cttcccagca agattatcag tagtaatgcc ggctttgccg aagccaagct gaatctctgg
      361 gaccgattat tcggaacggc gggtatcagg caggacgaat ataatgtgtt cggcagtgcc
      421 acgacgtatc gcgcaacggc gggatacctg ctgaaggaaa cgggaacgaa actgcgcggt
      481 agctatggga ccggatttcg cgccccgacg atcaatcaac tcttctttcc cgactttggg
      541 aatgcgaact tgaagccgga aaaaagtcga agtatggacg ttgcgctcga tcaatcgctg
      601 ttcaaggatc gtgtgtttct cagcgtgggc tatttctgga cgaaatacca gaacctcatt
      661 ctggcggtgt ttgatcccat cgggtgcaat accctatttg gcttctgcgc tcagaacatc
      721 ggagaagccc gagcgcaagg tattgaggcc agtgcgaagg tccagctcac ccgggaccag
      781 ccctggatca agagcctcga tctgcagttg caatacacac agaccagcac gagaaatctg
      841 acagacgatg tccgcacgag gctgccacgt tggccgctca accaatggtc cgccatactg
      901 agctaccagc cgatcgaagc tctgcgggca aatcttgagg ctcgcttcgt gggtcagcgc
      961 ttcaacgata ccaacaacga agattcactc ccacacttct acgtatggaa cgcttcgttc
     1021 agctatgacc tcaataagac agtgcagatt tatggacggg cggacaacat cttcaaccgt
     1081 aaatacgaag aggtgttgtt cttcggtacg ccggttcggt ctgtatttgg cggggttcga
     1141 gtcaacttcg acatgcctat ttgatgaggc agagcacgat gacgtattcc cgaggatgcg
     1201 tcacgtcgcc gaagtcggac acgcggtcgg agaaacggga tgaggggtcc aatctcgaaa
     1261 gtctgcgaat gctgtaagca aggctttacc tgcggccaat acggttgttg gtgcgccaag
     1321 gtcgaagtga acgagcagca gatgacgtgg atcgagaagt ctttccatga ttgtctttgt
     1381 ccgctgtgcc ttcagcggat cgttgatggc gaggtaggac cggcctctgt gttagaaccg
     1441 gagtaggagc caccgtgcgt attacaaaag tgtataccag aaccggtgac ggtggcagta
     1501 cccggctggc cggaggccag caggtctcaa aagactgcct ccgtgtcgag gcgtacggca
     1561 cggtggatga gttgagtgcg tcggttggcc ttgcgcgggc gatgaatgag gaagcgagcc
     1621 aagtggtcgg tgcggccaag cggctcgagc gagatctccg ttgggttcag aacaaactgt
     1681 tcgacgtcgg tgccctgctt gccacggctc cgggacagac cttcaagaat atgccgacga
     1741 ttagatcaca ggataccgtc aaactcgagc gcatgatgga ccgatgtcaa aaagacttgg
     1801 ctccgctcaa ggagttcatt cttcctggcg ggggaaaggt ctcggcaaca ttgcatcagg
     1861 ctcgtacgat ctgccggcgc gcagagcgta tttgtgtcag cctggccaag gatgaaccgc
     1921 ttgatgccga gatcatcaag tttttaaacc ggctgagcga tgcgctcttt gtgctggctc
     1981 gttgggtggc caagacacaa ggggaacccg aattcttgtg ggaacgaaac cccgacgaaa
     2041 aggattaggg ggatgaacgc gcgcaccctc atgattcagg gaaccgcctc gcatgtcggg
     2101 aaatccgtca tgacggccgc cctgtgccgg atattcgttc gtcgtggact gcgtgtggcg
     2161 ccattcaaag cgcagaacat gtcgaacaat tcgttcgtca cgccggacgg aaaagaaatc
     2221 ggccgggcac aggctgttca agcggccgct tgccgcctgg cgccgcaggc ggacttcaat
     2281 cccatcctga tcaaacctga atcacatacg gcggcacagc ttgtcgttgg gggagaggtg
     2341 gccggatcgc tgtgcgcgaa ggattttggt gcggtgcgtc gtcagtattg gaccacggtg
     2401 caggaggcct ttgggcggct gtcgcacaca tttgacgtgg tggtgctgga aggcgccggc
     2461 agtcccgccg aaatcaatct gaggcactgc gacgtcgtca atatgcagat ggcccgccat
     2521 gccggcgcgc ccgttgtatt ggtaggggac atcgaccgag gcggggtatt tgcctcactc
     2581 gtggggacct gggctctgtt ggatgaggcc gatcgtcgac atttgaaggc gttcgccgtc
     2641 aataaattcc gaggagatgc gagtcttctc gagaccggcg tcgtccgcgt cacacatgaa
     2701 acgggaatgt cgtgcgcagg ggtcgtccct cattggggag acctagaggt tcccgaggaa
     2761 gattcactgg gatgggatgg ctggcatggc cggacgtcaa agcgggcaga ccgcctcgtg
     2821 attggcattg tggatgtgcc tggcatttcc aatttcaccg acttcgaccc cttggccatg
     2881 gaacctgatg tggaactggc tcgcctgacc gagtcgacgg accgtccgtt ggacgccgtc
     2941 atttttccgg gaacaaaaca cacggttcag gccctcgggt tcgtccgcca acgggatttc
     3001 gatcgtcttg cccgccgcgt gttggctgaa ggagggacgg ttggcgggat ctgcggcggc
     3061 tttcaactgt tggggaaagt cattcgcgat cccctgcatc ttgaatcaga acacggtcac
     3121 atagagggcc tagggatgct tccggtcgac accacgtttg cgtttccaaa aatcgtcgag
     3181 caggtcacag ggcaacatgt cgagagcggc tgcaccgtgg tgggttatga ggtcagaatg
     3241 ggacgaaccg tcgttgccct tgaggccagg ccgtttttgg aatttcagga tggccgcagg
     3301 ggcacaacca cccgcgaggg gatttgcgct catgacggcc ggctctttgg cacgtacctg
     3361 catggtctgt tcgatcaggc tccatttcgt cgctggtggt tgaatcgatt gcggacatcc
     3421 aagggatgga cggggctgcc ggagacgcac gggctgtctc tcgatgcgcg ccttgaccgc
     3481 ctggccgatt ttgtcgaaca acatctttcc gtgtccatta tcgatcgact cctggaggaa
     3541 ggcgtttcgt atggcacccc gtaaaacaga atcgcatctc gtgttcgtcg tcggaggcgc
     3601 ctcctccgga aagagcgagg ttgcgcttca attggcgtgc aagggaatca gcaaaaacgc
     3661 tcctcgtgcc tttgtggcga caggcgaggg gttggatgaa gagatgacga tgaaaatcgc
     3721 ccgccatcgg caatccaggt cgtcggactg gaacacggtt gaagtcccgc tggagctggc
     3781 cgcctggtcg aaacgctacg gacaaggtta tcgcacgatt gtgattgatt gtctgaccat
     3841 gtggctttcg aaccggtggc gtcaaggaag caaggaacag gagatcctcc aagacggacg
     3901 aacgtttctc acggcgctgc gaggccttga ggcgcgtaca gtgatcgtct caaatgaatt
     3961 gggcatgggg ctggtccctg gtgaggccga ctctcggcgg ttccgggaag tggcgggagc
     4021 gttgaatcgt ctcgtggcca aagaggcgga tgaggcctat tttgtggtaa gcggaattcc
     4081 tcttcgattg aaataaagga accccatgag gctgaccgag gttttgaatc aaatctgcga
     4141 cgtgaatgcg gaagtggctg caaggacgca gcgccggcta gatcgcttga cgaaacctca
     4201 gggcagcttg ggtcggctcg aacaacttgc cgtccagtac tgtgcgatca cgtgcgagga
     4261 caagccggcc gtcccacgcg ggagggtcta tacctttgcc gccgatcatg gtgtggtcga
     4321 cgaaggtgtg agtgcttatc ctcgagatgt gacggcacaa atggtgctga attttctgcg
     4381 agggggtgcc ggcgtcaacg tgctggcccg acacgccgga gtcgaggtgc gtgtcgtgga
     4441 catcggagtc gcgcatgact tcggccccat tgctggcctg atgaaccgga aattgatcag
     4501 gggcacacga aatatggtgc atggggaggc tatggatcgg cgaactgccg aggaggccat
     4561 cgagatcggg atcgaactgg cgtccgaggc tgtggaagaa ggtgtgacgc tgatcggcac
     4621 cggtgagatg ggtatcggca acacgacggc gagcgcggcg atcgctgcgg tcatgactgg
     4681 ggcgtcggtt gtgcaggtca cgggtctggg aaccgggatc gacgaactcg gccgcacgcg
     4741 aaaaatttcc gtcattgagc gggcgctggc ccgtcatgcg cccaatgctg acgacccggt
     4801 cgatgtcgtg gcaaaaatag gcggcttgga aattgcgggc atggccggcc tcatgctcgg
     4861 tgccgcagcc gcgcgcatac ccgtcgtctt ggacgggttc atcgccggag cagccgcatt
     4921 gatcgcatgt ggtctccagc cccggtgcgt ccaatatatg atcgcctctc accgatccgt
     4981 cgagagaggc catcgcctgg tgcttgagaa actgggctta gagcccctat tcgacctgga
     5041 tcttcgactc ggggagggca ccggagcctg tcttgggatg agtctcgttc aggcagccgt
     5101 caaagtgctg acggagatgg cgacgttcag cgaagcgggt gtttcggagc gtaaccagtg
     5161 acgaccagtc tcaagccgtt catttttgcc tggcagtttc tcacggccgt acctctgagc
     5221 cgggaccttc atcatccggc gcctcacgag ttggccggat ctatgttgtg gtaccccttt
     5281 gtcggaatcc ttcttggagc gttcatggtc atgacggaca tgctcttggc ccgatggctg
     5341 tcgccggaag tggttgcagc gctgctgatc ttgttgcttg tcgtgatgac ccgaggcctg
     5401 catcaggacg gcctggctga cacgctcgac ggattggctg gaggacgcat gccgaccgat
     5461 cgcctgacta tcatgcgtga tcctcgtatc ggcgccatcg gcgcgaccgg tttgtttgtc
     5521 tcattgatcc ttcgttacag cgcacttgtc tcactgcctg agccgctgaa atttccagct
     5581 ctgctctgta tgccggcaat cggccgctgg gccatggttg tcggtgccta cggggcttca
     5641 tatgcgcgag ctgaaggagg tttggctgcc ccatttttgg cacacctgtc agcgcggcac
     5701 gtcctcggcg ccacggtggt tgtcgtgtct ctgatggcat ggtctcttgg cttcgctgct
     5761 gcattgatag tcctgctgat cggcggcata ttgagtcggc tgattacgat gtgctgtcga
     5821 tattactttg ctggcatcac cggcgacacc ctcggagcga caaacgaggt cgttgaaatc
     5881 agcttccttc tgttcatgcc atttctcacg tatctgccgt gactcctatc gatttcttgg
     5941 cagcttgcat ggtcgatgcc gtgatcggtg atccgcctgc gtggcctcat ccggtccgat
     6001 ggatgggcag actgatcaca ctcgtggaaa aatgggtgcg ccgatgctgt gttggatcag
     6061 gctcgcttcg cctcaccggg gttgcgctgg ccctgggcct gccgagcctc gccttttgtg
     6121 ccggttgggc cctcatggct accggtacgg cggtgcatga ctggatcggg cgcggtattg
     6181 gaatttggtt ggcatccact gtgctggcct ggcgcgatct cgtcgatcat gttcgcgccg
     6241 tgtcccgtgc gctgaaagcc ggtacgttgg atggggcacg tcactcggtt ggcttgatcg
     6301 tgggacggga caccgaccgc ctgtctgaac cggaaattgt gagggcgacg gtggagacca
     6361 ttgccgagag cgcgtcggac ggcatcattg cacctgtctt ctatctagtc gtgggggggc
     6421 cgcccttggc cttagcctac aaggccgtct caactctcga ttcgatgatc ggtcatcaag
     6481 atacagccta tcgggatttt ggatgggcct ctgccaagct cgatgatgtc gtcaattgga
     6541 tccctgcgcg gcttactggc tggttgatcg ccctcgcggc cggggccgtg tgctggaaca
     6601 cacgatcggt ccatcgaagc ggggcactgt tgctgcggga cgggcacaag catcctagtc
     6661 cgaacagcgg aagaccggaa gcggccatgg ccggcgcact cggtattcag cttggaggaa
     6721 caaactttta tgcaggactg cgacatgacc ggccgctgct cggggacgca aaggagccct
     6781 tgttagcggc acacattgat cgagcacagc aaatcatgac cgctgcctat ctaatcgcca
     6841 ttgtcgcgtg tctggggatc ttgtggcgct aaagaacgtg ctcgtccatg gaggcaatgt
     6901 gcacgccgta gcccgtgacc tgcgacgacc gatcggctcg atcctcgact tcagtgcaag
     6961 cattaatcca ttgggaccat cgcctcaagt caaacgagca ctggttgccg cagcaccgct
     7021 gatccatcac taccctgatc ctgattgttt caaccttaag caggtcatgg cgcgccattg
     7081 gaagatctcc ccggattgcc tcgttatcgg aaacggctcg agtgagctca tcgatgtcat
     7141 tccgcgcgct ctgggtatcc gctcagcctt gatcctcggt ccaacgtatg gggaatatgc
     7201 gcgagccgtt gatcgttctg gtggtcgata tcagatggtc ttggccagtc ggaacgaagt
     7261 gtacaggccc ccgctggagc aggtcacgca gcgtttgcag ggacaatgga gcggcccacc
     7321 cgtggcgatt gacgccgtct tcatctgtca tcccaacagt ccgaccggca ggccatgtga
     7381 tcttcaacag ctggaggatc tcttccaagc ggctgagcgc gggcgcgtat gggtggtgtt
     7441 ggatgagtct tttatcgact attgtgacgc gttgacctgt gtgccgaggt tgcagacata
     7501 tccacgtttg attatcttga gaagttttac caaattttac gggatgccgg gactacgaat
     7561 cggctacagc atgtcgtcgc gatccatcgc agccatcctg aaagagcacc agccgccctg
     7621 gtccgtcaac acggtggctc aacaggctgc tgaagcggcg atcgctgacc aacgtcatgc
     7681 gcgacgttgt ctcgcgtacg tcagccgaga acgcgcgcgc atgacgaagc agttggccgc
     7741 gctggcaggt gtgacggtga ttccatcttc cgcgaatttt gtctttgccg agctgccgcc
     7801 ctcctgtggc gccggcctga ttaccgcagc tcttcgacga cgaggcatgc tgatacggga
     7861 ctgctcgtca gtggagggct gcggtccacg aagcatacgc atggcggtgc gaaccaaggt
     7921 agataacgat cgcctgctcg cggccttggc agaggtactc gagcgatagc atgagccgac
     7981 gtaacacgaa gacgggaggc gtcaagtatc gagtccgtgg gaatacct
//
LOCUS       DASTQY010000182         6140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_446643, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000182 DASTQY010000000
VERSION     DASTQY010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446643"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..843
                     /locus_tag="VFR82_06040"
     CDS             88..843
                     /locus_tag="VFR82_06040"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4501195.1"
                     /translation="MRLTILGSGTNVHRTRAAAGYLVQTDQTLLLDFGPRTLMNLIKT
                     GVDRHRITHILFSHYHADHFSDFITYLFDEIIYTKFEGGNRPPLTVMGPRGTTRLFQT
                     MFGMLPGFDQAPFPLRYRDLSDRALRLGSTKIIPHTVLHTPDLHCLGYRIEYRGHALA
                     YSGDSQYCASLVRLCGDVDLAVLDCSFPANRPNPAHLHAGQCGQVAHEAGAGKLVLSH
                     FYPIAERYDVKAQAAEAFEGPIVAARDLLHIKL"
     gene            complement(1138..2001)
                     /locus_tag="VFR82_06045"
     CDS             complement(1138..2001)
                     /locus_tag="VFR82_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEU4501196.1"
                     /translation="MMVIPESQIRALIRLLADEDEKIVQTISGRLIDIGPSAVPLLQE
                     AEIEQPEMAERIASVLEEIRGGKLEDELITQAALPDDRMDLEHGSFLIARYAYPVLDV
                     VAYSRQLDEMAQEVRDRIGPRASGEETVKTLNRYLFTEQGFKGNTKNYYEVENTYLNR
                     VIDRRTGIPISLSIVYLLIGKRLRLPVHGIGMPGHFLVKYESDRYKIFVDCFNGGALL
                     TEKNCQRFLTEAGYGFDERYLQHSPVRAILSRMIKNLLAIYVKLGDPIRQTRLTKFME
                     ILGCEIKEGGL"
     gene            complement(2067..2846)
                     /locus_tag="VFR82_06050"
     CDS             complement(2067..2846)
                     /locus_tag="VFR82_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4501197.1"
                     /translation="MHEYWQEISALTMRWVRRLSREKFSMLFTLVQPMLFWLIFFGNL
                     FQRAADSQVTQAPNYISFLAAGVVVMTVLNNGLAGGVDLLFDKENGFLERLMSTPIHR
                     TSVIMSRFIFVMTITSLQVLVILGVAFLFGVQPATGLLGIATILLIGMMFGVGLTAIS
                     MAMAFSVKSHGDFFSVLGFLSLPMIFLSSALVPLTAMPAWMSVLAKLNPMTWAIDAVR
                     PLILSNWSEALPHVGMVVVVMLLFDALCLYGGAKAFRRAMG"
     gene            complement(2870..3838)
                     /locus_tag="VFR82_06055"
     CDS             complement(2870..3838)
                     /locus_tag="VFR82_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4501198.1"
                     /translation="MHGAIAIDVSHLSKSYDGHRAVADISFQVHVGEIYGLLGPNGAG
                     KSTTLRTLITLLAPSAGRATIFGLDCVRDADRVRRLIGYVPQERAIDRFLTGREHLEL
                     LAALYHLPKDNAQRRIAELLKLVELEADADRPAKTYSGGMKRKLDIACGLLPDPKVLF
                     LDEPTLGLDVQSRLRIWDYIRMLKARGLTVVMTTNYLDEADQLCDRLAIIDGGVIKVI
                     GSPVELKIGLGGDIVSLTLKEAERISSLESALKGQPAIKAVRATSKGLDIRVESPEKA
                     LPTILELANRLGCTVEFVQYNRPRLDDVFMAHTGRRIEDAADTHVE"
     gene            complement(3838..4617)
                     /locus_tag="VFR82_06060"
     CDS             complement(3838..4617)
                     /locus_tag="VFR82_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEU4501199.1"
                     /translation="MRQGISSLLPLVSLAVAPLTILSAIGYRDRMQTDHERDHHEPEP
                     SEKSSPFSIPEHIPVFALPNVVLFPKTYLPLHVFEPRYRQMVDDAVMGGQCIGIALLK
                     EGWESEYYGNPPVFSTGCVGRLVSVQPLADGRSNILLQGLERFEITLESYEKPYRQAS
                     ITLKPRGAEASLDPGVRQRLVTTLEEYLRSRQDAPTWQGWFREDVSDDILVNTLSTYL
                     DCTPLEKQFLLEAVTLHQQARRLSDLIQFLMHDQHGAKGWG"
     gene            4681..5799
                     /locus_tag="VFR82_06065"
     CDS             4681..5799
                     /locus_tag="VFR82_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEU4501200.1"
                     /translation="MTSTWQLIFLLGWLAIVLPSIAADTTSFVPAQEGYRYTFPRDHG
                     SHDDFRTEWWYYTGNLVSNDGRPFGYQLTFFRRSIPQEQVKTLPSRWSITQLYLAHFA
                     VSDLKNGRFHVFEKLSRAGVGKAGAEAERLHVWIDRWRAQSFQADMASQTLQASDDDL
                     EIDLTVVPEKAPVIHGKGGVSRKGAESGQASHYYSLTRLATSGRISLGNESFNVTGTS
                     WMDHEFGSADLGEDLVGWDWFSLQLADHTELMLYRMRRSDGSPDPVSSGTFIEQDGRV
                     QHLTLGEFMLDPITTWTSPASKARYPSTWRLSIPSKGLSLALIPRMAEQELTTNRSTQ
                     VIYWEGAIEASGTVGGKPIAGRGYMELTGYAERFSKKL"
BASE COUNT         1357 a         1771 c         1702 g         1310 t
ORIGIN      
        1 ggcgctgtcc cggcaagaag tccatactgc cagcacattg ttgctccgga tccgtgacag
       61 tttccaaaag ggaggacagg cttgagcatg cgtctcacca ttctcggttc aggcacaaat
      121 gtccatcgca caagggcggc agccggatac ctggttcaga ctgatcagac gcttttgctg
      181 gatttcggcc ctcggacgct gatgaatctg atcaaaaccg gtgtggaccg gcaccggatc
      241 actcacattc tattttcgca ttaccatgcc gatcacttct cggatttcat tacgtacctc
      301 ttcgacgaga tcatctacac caagttcgaa ggtggcaaca gaccgccctt gacggtgatg
      361 gggccccggg gcactacccg tctgttccag accatgttcg ggatgttgcc ggggtttgac
      421 caagcgccgt tcccgctccg gtatcgtgat ctgagcgatc gggccctgcg tttgggctcc
      481 acgaagatta tccctcacac tgtgttgcat acccccgatt tacattgcct tggctaccgg
      541 atcgaatacc ggggccacgc gcttgcatac tcaggggatt cgcaatattg cgcgtcgctc
      601 gtgcggctct gcggcgacgt ggatctcgcg gtgctggatt gttcgtttcc ggcgaaccgg
      661 cccaatccgg cccatctgca cgcaggccaa tgcggacagg tggcccatga agccggggcg
      721 ggaaagctgg tgctctcgca cttctatccg attgccgagc gttacgacgt gaaggcgcag
      781 gcagccgagg cgttcgaggg accgatcgtt gctgcgcgtg atctcctgca catcaagctc
      841 taaaaaaact gacacaggca agcgaggaga cgcggaggcc ataccaagct ttgcttgatc
      901 cgctcgcttc gaccaccgag cgggtaaggc actttgcctc cagttcactt ggtacgtcga
      961 gcttttgggc gatgcgagga agagcttggg tccaagcgcg tcgcgggctt tcgatcgcga
     1021 aacgatggga gggtaaggtc ggtaaaaagc tttcgctagg attggacggg tgagcttggg
     1081 aaaggcggat cctgcacgcc gggggtttgg cgggtgagaa agccggcttt cagagtccta
     1141 caatccgcct tccttgatct cgcagcccaa aatttccatg aacttggtga gacgggtctg
     1201 cctgatggga tcacccagct tgacatagat cgccaagaga ttcttgatca ttcgggaaag
     1261 gatcgcacgg acagggctgt gctgaaggta gcgctcatcg aacccatagc cggcctccgt
     1321 cagaaaacgc tgacaatttt tttccgtcaa cagggctcca ccattgaagc agtcgacgaa
     1381 aatcttgtat cggtcggatt cgtacttgac taagaagtga ccaggcatgc cgataccgtg
     1441 aaccggcaac cgcaagcgct tgccgatgag caggtagacg atggacaaac tgatcggaat
     1501 tccggtgcgc cggtcgatga cacggttgag ataggtattt tcgacctcat agtagttctt
     1561 ggtattgcct ttgaagcctt gttccgtaaa caagtatcta ttgagggtct tgaccgtttc
     1621 ctctcctgac gcgcgcggtc cgatgcggtc acgcacttcc tgcgccattt cgtcgagctg
     1681 tcgactatag gccaccacgt ccaagaccgg ataggcatag cgtgcgatca aaaatgaccc
     1741 atgctccaag tccatccggt cgtcgggaag ggccgcttgc gtgatgagtt cgtcctcgag
     1801 ctttccacca cggatttctt ccagtacgga cgcgatccgc tctgccatct caggctgttc
     1861 gatttctgct tcctgcagga ggggaacggc tgaaggaccg atgtcgatta gccggccact
     1921 gatcgtttga acgattttct cgtcctcatc agcgaggagc cggattaggg cgcggatctg
     1981 actttctgga atgaccatca ttcccaccct tcttaccagc ccttccgttt gagggtcaac
     2041 ataccagggg gtctgtgcct tgcccctcat cccatggccc ggcgaaacgc tttggcacca
     2101 ccatagaggc acagcgcatc aaagagcagc atcacgacta cgaccatccc tacatgcggg
     2161 agcgcctcgc tccagttgga aaggatcaac ggccgcaccg catcgatggc ccatgtcatc
     2221 ggattgagct tcgccaggac gctcatccag gccggcatcg ccgtcaaggg aaccaacgct
     2281 gaactcaaga atatcatcgg cagggaaaga aaacccaaca cggagaagaa atcgccatgg
     2341 ctcttcacgg aaaacgccat cgccatggaa atcgccgtga ggcccacgcc gaacatcatg
     2401 ccgatcaaca ggatggtggc gatgcccaat aagccggtcg ccggctgaac gccgaagaga
     2461 aatgcgactc cgagaatgac cagcacttgc agcgacgtga tcgtcatgac aaaaatgaag
     2521 cggctcataa tgaccgacgt ccggtggatt ggcgtggaca tgagccgttc tagaaatccg
     2581 ttttctttgt caaacagcaa gtccactcct cccgcgagcc cattgttcag cacggtcatg
     2641 accaccaccc ccgccgctaa aaagctaatg tagttcggcg cctgtgtgac ctgggaatcg
     2701 gccgcccgct gaaacagatt tccgaagaag atcagccaga acaacatggg ctgcacgagg
     2761 gtgaagagca tgctgaattt ttcacggctg agccggcgca cccaacgcat ggtcaacgca
     2821 ctgatctctt gccagtattc atgcatagcg gagtttttcc aagagttgtt tattcgacat
     2881 gagtatccgc tgcgtcttcg atgcgtcgtc cggtgtgagc catgaacaca tcatccaacc
     2941 tgggccggtt atactggacg aactcaaccg tgcatcccaa ccggttggcc agctccagaa
     3001 tggtaggcag tgccttctcg ggcgactcaa ctcggatgtc cagtcccttc gacgttgccc
     3061 tgacggcctt gatagcaggc tgacccttca aggccgattc gagggacgag atcctctccg
     3121 cctccttgag cgtcaaggag acgatgtcgc cacccagtcc gatcttcaat tcaaccggtg
     3181 agccgatgac cttgatgacc ccgccatcga taatcgccaa tcggtcgcag agctggtcgg
     3241 cttcgtcgag ataattggtc gtcatgacca cagttaaccc ccgtgctttc aacatacgga
     3301 tgtagtccca aatccgcaga cgactctgaa catccaaccc caatgtcggt tcgtctaaga
     3361 acagaacttt tggatcgggc aggagaccgc aggcgatgtc gagcttgcgc ttcattcctc
     3421 cggaataggt ttttgccgga cggtcggcat ccgcttcgag ttccaccagc ttaagcaact
     3481 cagcgatccg cctctgcgcg ttatccttgg gcagatggta caaggcggcc agcagctcga
     3541 gatgttctct gccggtcaga aatcggtcga tggctcgctc ttgcggcacg tagccgatca
     3601 atcgccggac acgatccgca tcccgtacac agtcgaggcc aaaaatcgtc gcacgtcccg
     3661 cagaaggcgc gagcaaggta atcagcgtac gcagggttgt gcttttgcct gcgccgttgg
     3721 gacccaacag tccgtagatt tcgccgacat ggacctggaa ggaaatgtct gcgacggccc
     3781 ggtgaccgtc gtaagacttg gaaagatgag agacgtcgat cgcaatcgcc ccgtgcatca
     3841 cccccagccc ttcgccccat gctgatcgtg catcaggaat tggatcaaat cgctgaggcg
     3901 gcgagcctgt tgatggagag tgacggcttc caacaggaat tgcttttcca gcggggtgca
     3961 atccaggtac gtggataagg tgtttacaag aatatcgtca ctgacatctt cgcggaacca
     4021 tccctgccag gtcggggcat cctgacggga ccgcagatac tcctcgagcg tggtaacaag
     4081 tcgctgtcgt acaccaggat cgagcgaggc ctccgcccct ctcggcttca gcgtaatcga
     4141 ggcttgacgg tagggcttct catacgactc cagggtgatc tcaaaccgct cgagcccctg
     4201 gagcagaatg ttggaccggc cgtcggccag cggctgcacg ctgaccagcc gcccgacgca
     4261 tccggtcgaa aataccggcg gattaccgta atactcggat tcccagcctt ctttgagcag
     4321 cgcgatcccg atacattgcc cacccatcac ggcatcatcg accatttggc ggtaccgcgg
     4381 ctcaaaaaca tggagaggca gatacgtttt tgggaacaag acaacgttcg ggagcgcaaa
     4441 aactggaatg tgctcaggaa tggaaaatgg cgagctcttt tcggacggct ccggttcatg
     4501 atgatcacgc tcgtgatcgg tctgcatgcg gtcacgatag ccgatggctg acaaaattgt
     4561 caacggcgcg accgcgagac tcacgagcgg aagaagcgac gaaatccctt gtcgcaactg
     4621 gcgatttgtg acttcatgca gctacatgtt ataagcgcac gcatcatgcc cggaatacgt
     4681 atgacttcaa catggcaatt gatcttcctg ctcggttggc tcgcaatcgt ccttccgtcg
     4741 atcgcggcgg acaccacctc attcgtacct gcgcaagaag gctatcgata tacattcccg
     4801 cgcgaccacg gttcgcacga tgacttccgc accgaatggt ggtactacac cggcaacctc
     4861 gtgagtaacg atggccgacc attcggctat caactgacct ttttccgtcg cagcattccg
     4921 caggaacagg tcaaaacctt gccctcacga tggtccatta cccaactcta cctcgctcac
     4981 tttgccgtga gcgacttgaa gaacggacgg tttcatgtct ttgaaaagct cagccgtgcc
     5041 ggcgtcggca aagctggggc tgaggcggaa cgtctgcacg tgtggattga ccgatggcgc
     5101 gcccaatcct tccaagcaga catggcgtca cagacccttc aggcgtcaga cgacgacctg
     5161 gagatcgact tgacggtcgt gccagaaaag gcacctgtca ttcatggcaa agggggtgtc
     5221 agtcgaaaag gtgccgagtc aggccaagcg tcccattact actcgttgac gaggctggcg
     5281 acgtcgggga gaatcagcct gggcaatgaa tcgttcaatg tgactggaac cagttggatg
     5341 gatcatgagt ttggttctgc cgatctcggc gaagacctcg tcggatggga ttggttcagc
     5401 ctccagcttg ccgatcacac tgaattgatg ctctatcgca tgcgccggtc cgacggatca
     5461 cccgatcctg tttcaagcgg cacttttatc gaacaggatg gtcgggttca gcatcttaca
     5521 cttggggagt tcatgctgga tcccatcacc acctggacca gtcctgccag caaggcacgt
     5581 tatccctcga cctggcggct atccattccg tcgaaaggtc tttcgcttgc acttattcct
     5641 cgcatggcgg agcaggaact taccacgaac cgcagcacac aggtcatcta ttgggagggc
     5701 gccatcgagg cgtccggcac cgtgggagga aagccgatcg cgggccgcgg ctacatggag
     5761 ctgaccggtt atgcggagcg cttctcgaag aaattgtaga gttactcgcg gaggtacccc
     5821 gccgctccgg cgcttcgccg cagccacact aagtctttca tcttctcgat gccgaaactt
     5881 gttctgttcc gcgtttcgca tgaacggaac ttcaaacatc ggcatcccca agctggttgg
     5941 tcgcatctga ttgacaagtg tggtacccgc atctcagcgc gagtggtgat gtgagacacc
     6001 tccccaaact gagggaagga agggcaaaaa gggtcggcag atgtggagtg cacgtgtatg
     6061 gcagttcgcc taccccgcgc tgatcgggct gacgcttctc agcatcggct gtcaaaaaga
     6121 cagcgaagcg attgtctcca
//
LOCUS       DASTQY010000183         4147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_251996, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000183 DASTQY010000000
VERSION     DASTQY010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251996"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFR82_06070"
     CDS             <1..556
                     /locus_tag="VFR82_06070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4501201.1"
                     /translation="EADVRDAQAVHQLVAAFIRDSTPPVCFVCCAGVGSGRLLLKLST
                     DEWVDVMATNLTGTFHSLRAMGPALLAQGGGSIVVLGSHAGSQGSIGQAAYAASKAGL
                     IGLVKTAAVEWGPYNICINLVLPGWQHTRLTTEAMSRGQQWKDHALGRPPALEEVVRT
                     VMHLVELKDVSGQVWNCDSRSLSA"
     gene            553..1311
                     /gene="bioD"
                     /locus_tag="VFR82_06075"
     CDS             553..1311
                     /gene="bioD"
                     /locus_tag="VFR82_06075"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEU4501202.1"
                     /translation="MIQRRVAGGAGVFVTGTDTGVGKTLVTAALAIRLRRLGRTVGIM
                     KPIETGVTSSRLVQSDAARLRAAVDSEETLGAICPYQFEQPVAPLGAAHMERRSIDPR
                     VIRQVYRLLASRYQSVVVEGIGGVRVPITADADVMDVMLGLKLPAIVVGRAGLGGINH
                     ALLTIDALHRRRIPVVALILNRTHPVRSSVTRLQEKTTLDALRKEAGVPVLGPLPYVP
                     GLVRRFRQSASRVANMSAITTLAKLVRASARGGR"
     gene            complement(1283..1921)
                     /gene="thiE"
                     /locus_tag="VFR82_06080"
     CDS             complement(1283..1921)
                     /gene="thiE"
                     /locus_tag="VFR82_06080"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU4501203.1"
                     /translation="MSRNQRTSALSGLYVILDSSRRPDRPLIDVLNGAATAGVRIFQY
                     RNKTASMKEAFGEALPLRNRARELEVLFLVNDRCDLALAVDADGVHLGQNDLPVAQAR
                     RILGPETVIGLSTHNDRQVEAADRLQPDYIGFGPIFAPGSKTDHAPIVGIDALRRIRL
                     LTRLPIFAIGGIHLEQVRAVIEAGADGIAVISALAGAPDLEVAVSALLEQMP"
     gene            1979..2365
                     /locus_tag="VFR82_06085"
     CDS             1979..2365
                     /locus_tag="VFR82_06085"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU4501204.1"
                     /translation="MSCLFPVQSPGPLVTAVVLFLISLMSGCESREQTVRIVAEDFRF
                     TPAEVHVSSEYPVRLLIVNEGRERHEFKTALLAHQIGKSPGPSSSLPVRPNQKAEVLV
                     RTVTGVYVFYCAMRGHAGMNGTIIVE"
     gene            complement(2380..3720)
                     /gene="accC"
                     /locus_tag="VFR82_06090"
     CDS             complement(2380..3720)
                     /gene="accC"
                     /locus_tag="VFR82_06090"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU4501205.1"
                     /translation="MFKKVLVANRGEIALRVIRACKELGIKTVAIHSEADAASMHVRA
                     ADERICVGPAESALSYRNIPNVLSAAEITGADAIHPGYGFLSENAHFAEVCESIGVKF
                     IGPTSENIALMGDKAKAREIVGRRGLPVTPGSPGELKSEQEAREAAAKIGYPVIIKAT
                     AGGGGRGMRVVNSTDELGRAFQAAQAEAKSTFGNDSVYLERYFLEPRHIEVQIAADAR
                     GHVVYLGERDCSIQRRHQKLVEETPSPAVDEKLRREIGRVAVEAVKAVHYRNLGTVEF
                     LLDKDHNFFFMEMNTRIQVEHPITEMVTGIDLIKEQITLANGQPLSFKQPDIRLTGHS
                     IECRINAEDPEKFTPSPGVISRYCAPGGFGVRVDSAMESNAVVVPHYDSMIAKVITHG
                     RDRQEAMARMRRALDEFVIEGIKTTIPLHKRILNDPDFQKGHLSTTYLERFLAS"
     gene            complement(3807..>4147)
                     /gene="accB"
                     /locus_tag="VFR82_06095"
     CDS             complement(3807..>4147)
                     /gene="accB"
                     /locus_tag="VFR82_06095"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU4501206.1"
                     /translation="TAVSEAGQGTGQTVSQHTTPSSELAEDAAGHITITSPIVGTFYR
                     SPSPDADPYVEEGDFVKRGQVLCIVEAMKLMNEIESEVDGRITKILVESTKPVEYGQA
                     LFLIDPKATP"
BASE COUNT          772 a         1172 c         1285 g          918 t
ORIGIN      
        1 tgaggcagac gtgcgcgatg ctcaagctgt gcaccagctg gtcgctgcgt tcattcggga
       61 cagcacgcct cccgtgtgtt ttgtgtgctg cgcgggcgtc ggaagcggaa gactattgct
      121 gaaactctct acggatgagt gggtggacgt gatggcgaca aacttgacgg gcacattcca
      181 ttctctccgg gcaatgggcc ctgccctcct cgcccaagga ggcggatcca tcgtggtcct
      241 tggctcgcac gccggatcgc agggatcgat cggtcaagcc gcctatgccg cgtccaaagc
      301 gggcctgatc gggttggtaa agacggcggc tgtcgagtgg ggaccgtaca acatttgtat
      361 caatctcgtc ctgccagggt ggcagcacac gcgtttaacg acggaggcga tgtccagggg
      421 gcagcagtgg aaagatcatg ctctggggcg gcctcctgca cttgaggaag ttgtgcgtac
      481 ggtcatgcat ctcgtggagc tgaaggacgt ctctggtcag gtctggaact gtgacagtcg
      541 gagcctatct gcatgataca gcgacgagtc gcgggtgggg ccggcgtctt tgtgacggga
      601 accgataccg gtgtaggtaa gacactagtg actgcggcac tggctatccg gttgcggcgt
      661 cttggacgga ccgtgggtat catgaagcca atcgaaaccg gcgtcacttc ttctcggctg
      721 gtccagtccg atgcggcacg gctccgggcc gctgttgaca gtgaggaaac gcttggcgcg
      781 atctgtccct accagtttga acaaccggtg gccccgctgg gtgcggcgca tatggaacgt
      841 cggagcatcg atccgcgcgt gattcgtcaa gtctatcgtc tgctcgcgag ccgatatcag
      901 tcggtcgtgg tggaaggtat cggcggggtt cgggtgccca ttacggccga cgccgatgtg
      961 atggacgtga tgcttggact caagctgcca gccattgtag tggggcgcgc cgggctggga
     1021 gggatcaatc atgccttgtt gacgatcgat gcgcttcacc gacgccggat tccggttgtc
     1081 gcgctcattc tcaatcgcac gcatcctgtc cgatcttcgg tcacgcgtct tcaggaaaag
     1141 acgactttgg acgccctccg gaaagaggcg ggcgtgccag tgctcggtcc actcccctat
     1201 gtgccgggtt tggtgcgacg attccggcag tctgcttctc gggtcgcaaa catgtcggcg
     1261 ataactacgc tggccaagct ggtcagggca tctgctcgag gagggcgctg actgcgactt
     1321 cgaggtcagg tgcgccggcc agcgcagata tcacggcgat gccgtctgct cccgcttcga
     1381 tgaccgcccg aacctgttcg aggtgaatcc cgccaatcgc gaaaatgggc agtcgggtca
     1441 gtaagcggat tcttctcagc gcgtcaatac ccacaatagg agcatggtcg gtttttgagc
     1501 cgggcgcgaa gatcggtcca aagccgatat aatccggttg cagacggtcg gctgcttcca
     1561 cctgtcggtc gttgtgcgtg gacaatccaa tcacggtctc aggtccaaga atcctacgcg
     1621 cctgtgccac aggcaaatcg ttttgaccaa gatggacacc gtcggcatcc acggcaaggg
     1681 ccaggtcaca acggtcattc acgaggaaga gcacctccag ctctctcgct ctgttccgga
     1741 ggggcaaggc ctcgccaaag gcctctttca tcgatgccgt cttattgcga tattggaaga
     1801 tcctgacgcc ggcggtggcg gctccgttca acacgtcaat gagtgggcga tcgggccgtc
     1861 tcgacgagtc gagaatgacg tacagtccgg acagtgcgct tgtacgttgg tttcgggaca
     1921 tggtaggtgc attgtagcgt gacacgtgaa acagacgcca ggaacagccc gcatagacat
     1981 gagctgtctc ttccccgtgc agtcgccagg gccgttagtg acggcggtgg tattgtttct
     2041 gatctctctc atgtccggat gcgaatcgag ggaacagacc gtccgcatcg tcgccgagga
     2101 tttccgattc acgcccgccg aagtgcatgt ctcctccgag tatccggttc gcctccttat
     2161 cgtcaacgaa ggccgcgagc ggcatgaatt caagacggcc ttgttggctc atcagatcgg
     2221 gaagtcaccg gggccatcca gcagccttcc tgtaaggccg aatcagaaag ccgaggtgct
     2281 cgttcgcacc gtcactggcg tgtatgtgtt ttattgcgcc atgcgcggcc acgcgggcat
     2341 gaacggcacc atcattgtcg agtgagaacg ggtctgcggc tagctggcga ggaagcgctc
     2401 gaggtaggtc gttgacaggt ggcctttttg aaagtccgga tcattgagaa tccgcttgtg
     2461 aagcggaatg gtcgtcttga tgccctcgat gacgaactcg tcgagtgctc gccgcatcct
     2521 ggccatggct tcttgccggt cgcgcccgtg cgtaatgacc ttggcgatca tggaatcgta
     2581 atggggaacg accacggcgt tggattccat tgctgaatcg acccgaactc cgaagccgcc
     2641 tggagcgcag tatctgctaa tcaccccggg tgacggagtg aatttttccg gatcttcggc
     2701 gttgatccgg cactcaatgc tgtggccggt gagtcggata tcgggttgtt tgaacgagag
     2761 gggttgtccg ttggcgagcg tgatttgttc cttgatgagg tcgatcccgg tgaccatctc
     2821 cgtaatggga tgctccactt ggatgcgggt gttcatctcc atgaaaaaga agttgtggtc
     2881 cttgtcgagc agaaattcca cggtgcccaa attcctgtaa tggacggcct tgaccgcttc
     2941 aaccgccacc cgcccgatct cacgccggag cttttcatcc accgccggcg aaggagtttc
     3001 ttccacaagc ttttgatgcc ggcgctgaat ggaacagtcg cgttcgccca gatacacgac
     3061 gtggccacgc gcatcggcgg cgatctgaac ttcgatgtgg cgaggctcga gaaagtaccg
     3121 ttcgaggtac acactgtcgt ttccgaacgt ggactttgct tccgcctgag ccgcctggaa
     3181 cgcccgtccc agttcgtcgg tgctgttgac cacccgcatc ccgcgtccgc cgccccctgc
     3241 ggtcgctttg ataatgacag gatagccgat tttggcggcc gcctctcgcg cctcttgttc
     3301 gctcttgagc tcgccaggac ttccgggggt gactggtagt ccccgacgac ccacaatttc
     3361 tctggcctta gccttgtctc ccatgagtgc gatgttttcc gacgtcgggc cgatgaattt
     3421 gacgccgatc gactcacaga cttccgcgaa gtgcgcgttc tcggagagga agccataccc
     3481 ggggtgaatg gcgtctgcgc ctgtaatttc tgcggcgctg aggacgttcg ggatgttgcg
     3541 ataacttaaa gcggattcgg ccggcccgac acagatgcgc tcgtctgcgg cgcgcacatg
     3601 catactggcc gcatcggcct ccgagtggat ggcgacggtt ttgatgccga gttctttgca
     3661 cgcgcggatc acgcgcagtg caatttctcc acggttggcg acgagcactt tcttgaacac
     3721 gtcacatctc cgtcatggcg tggaggaggg ttctcaccga atccgagtga gacaaataca
     3781 tcgttgagca gacacgaacg cctggatcaa ggggtcgcct tgggatcgat gaggaagagc
     3841 gcctggccgt actcaacggg cttggtgctt tccacgagga tcttcgtgat gcgcccgtcc
     3901 acctccgact caatttcatt catcagtttc atcgcttcta cgatacacag aacctgcccc
     3961 cttttcacaa aatcaccttc ttccacgtaa ggatcggcgt ccggggacgg cgaccgatag
     4021 aacgtgccga caatcggaga ggtaatggta atgtgaccgg cggcgtcttc ggcgagttca
     4081 ctggacgggg tggtgtgttg cgagacggtc tggcccgtgc cctgcccagc ttcactgacg
     4141 gcagtgg
//
LOCUS       DASTQY010000184         4784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1646476, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000184 DASTQY010000000
VERSION     DASTQY010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646476"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VFR82_06100"
     CDS             complement(<1..522)
                     /locus_tag="VFR82_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU4501207.1"
                     /translation="MLLKRWLVGNPIKTAQAAHERLSKRLALAIFSSNSISSVAYATE
                     EILLVLILAGTSALAWSIPVSLAILFLVIVLTISYRQIIYEYPEGGGAYIVARSNLGE
                     MPALIAAAALMIDYVLTVAVSVAAGVAAITSAVPELFAHRQALGLISIVFIVVMNLRG
                     TRESGKFFAIPTYF"
     gene            841..>4784
                     /locus_tag="VFR82_06105"
     CDS             841..>4784
                     /locus_tag="VFR82_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU4501208.1"
                     /translation="MFFHFTRVYRPTCTLGVLAAAALLWAPFASAENPSSHAATNHQL
                     PAWAEQLKGQTIIEDTMSGKGERAAMVEQQHQRIMEHMTHDPQVQGVNTGMYNTSAMM
                     HQYGAGGQDMLLVSDPRVEPVAMTGGGKCPATAPVKQYNVSAINVEITLNQWLDFYPG
                     YMYVLDENLDKVRAEENTNKEARDKEGHDPGAVINGVQAQWIQPLVIRGNQGDCVKLK
                     LNNKLEGGEDVSLHIHGSAMVVSATGAAATTTNPDTIVAKEKTGEYEWYIHPSTQEGV
                     RQFHTFSNDRELTVMGLFGAFVVEPRGSSYLEPLGAGDPTPATSGWQTIIKNGTGPDF
                     REFVLIYHEVGDEAFRPLNKKGDFLPQRDPLTDAYRPGGRAINYRSEPFGINNMHVQH
                     EYFGFEDESMGYSSYTFGDPSPTIPRSYMGDPAKFRLVHGGSEVFHSHHPHGGTIRWP
                     RSPRAIDDMNLWATSTNGPVKYPVIRAKTDRVDVEVIGPSEALDLETECGSGLCQQLA
                     GDFLFHCHVAHHYVAGMWGYWRVYNTIQQGDMRNDVMPDLRELPDRKGRIKTPISSDK
                     LIGQTVDWFGTQFKIIDKGKSNWKGNPATITIKDWVEMQMPTPGKPGHKDDEKGQIVS
                     YDATVLDWTWNGNVAMSEKESTIDNPKYKSTHPGKRHPIMFEPLTGKVAWPHLTPHFG
                     KRVMFSPNHVGAPWLEMIRRDANGEESVDQAAPGEQGNWSLCPVNAGRKNYNVHFIKL
                     PIKIAKAQGKEPPVVDPNGLIYVLHEEEAAIRANDDLKYPLVVRGNIYDCVDWMLTSE
                     WDDDDYTNFQSSKINTHWHFLQFDNQASDGVITGFSYEQSVRPFTMLEKKNKKGLPMP
                     MNTALTAAAKKGSNSLSVKNAKQYHVGTLILVGADNVKGNEISRIKAINGNTITLAKG
                     LKNDHPANDIVTVEFVRQRFWVDADVGTVFWHDHAFGATTWPHGGFGTFIAEPVGSTY
                     HDPKTGKLVRSGPIADIHTNEPVGHGVNNSFRELMVQVHDTVPHTVNIVTAGNPPGQP
                     VEVALEAGKTVSFMMPEKLYMTPMPFLNGGTHTTGSGLNFRAGPIAQRLATNPDVSQA
                     FNSQIHGDPYTPLLRAYTGDTMVFRLLHTLMNETMTWTLAGHTFWSERYASDANRKNS
                     IHIGIAERYDLVVPEAGGPRHQAGDYIHFNGRSSKFSEGGWGIIRVYDKEQADLKKLP
                     AGFSVKGEIPKALPVCPADAPVKTFNVVAMDYPAMTFNAKAPEAIEVDFERKIQITNP
                     DAKIYALEEDAAKVSGGSHPMPLTLRVNVGDCVKVKLKNKMKEGKASFSAIG"
BASE COUNT         1143 a         1336 c         1414 g          891 t
ORIGIN      
        1 aaaatacgtg ggaatcgcga agaattttcc ggattctcgg gtacccctga gattcatgac
       61 gacgatgaac acgatcgaga tgagtcccag ggcctgccga tgcgcaaaca gctccggaac
      121 ggccgaggta atcgctgcga cgccagccgc cacgctcaca gccaccgtga ggacatagtc
      181 gatcatgagc gccgcggcag cgatcagcgc cggcatctcg ccaaggttcg accgggccac
      241 gatataggct cccccgcctt ccggatattc gtaaatgatc tggcgatacg aaatggtcag
      301 gacaataacc agaaacaaaa tggcgaggct gacggggatg gaccatgcca atgccgaggt
      361 gccggcaagg attaagacga ggagaatttc ttccgtggca taggcgacgg aggaaatgga
      421 attcgatgaa aatattgcga gagcgagacg tttggaaagc cgttcgtgtg ctgcttgggc
      481 cgtcttaatg ggattgccga cgagccagcg cttgaggagc atggcagaga ttatcgcata
      541 aacacgcatg gaacgagaga agcagaaggc ctctagccat agggcgaaag cctcagatca
      601 gcgattgcgg atgccgtcat ttccggtcca acggagaggt gtctggcaat ggagaaccct
      661 agtgcgatca aagggattga cagccctagg caacttaagt atctttccaa ttccctcgcg
      721 ccctagacag tgttttgcgg ggaatcggtc cattccatcg gtctttatac ggaggttctt
      781 acaggcaatt tacacttgca ttctgttagt ccacttatac attcgtaagg aggccagtcc
      841 atgtttttcc atttcacacg ggtctatcga cccacctgca cgctcggcgt gttggcagca
      901 gcggcccttc tttgggcccc tttcgcatcc gccgagaacc catcgtccca tgcggcaacg
      961 aatcatcaac ttcccgcctg ggcggaacaa ctcaaggggc agacgatcat tgaggacacc
     1021 atgtccggca agggcgaacg tgccgcgatg gtcgagcagc aacatcagcg catcatggag
     1081 cacatgactc atgatccgca ggtccaaggc gtcaacaccg gcatgtacaa cacctcagcc
     1141 atgatgcatc aatatggcgc aggtggccag gacatgctgc tcgtatccga tccgcgtgtc
     1201 gaaccggtag ccatgacggg cggtggcaag tgcccggcga ccgcccccgt gaagcagtac
     1261 aacgtgtccg cgatcaacgt cgagatcaca ctcaaccagt ggctcgattt ttatcccggc
     1321 tacatgtacg tgcttgatga gaatctcgac aaggttcgtg cggaagaaaa caccaacaag
     1381 gaagctcgcg ataaagaagg tcatgatccc ggtgccgtca ttaacggtgt gcaagctcag
     1441 tggattcagc cgctcgtgat tcgcgggaac caaggcgact gcgtcaagtt gaaactcaac
     1501 aacaagttgg aaggcggcga ggacgtcagc ctgcacatcc acggctccgc catggtcgtg
     1561 agcgcgacag gagcggctgc caccaccacc aaccccgata cgatcgtcgc caaagagaag
     1621 accggcgaat acgaatggta catccacccg agcacgcagg agggtgtccg tcagttccat
     1681 acattcagca atgaccgaga attgaccgtg atgggactct tcggcgcgtt cgtcgtcgag
     1741 ccgcgtggat cgagctacct cgagccgctc ggggcgggtg atcccacgcc ggccaccagt
     1801 ggctggcaga cgatcatcaa gaacggcacc ggcccggact ttcgcgaatt cgtgctgatc
     1861 tatcatgagg tcggtgatga ggcgtttcgt ccgttgaaca agaagggcga ctttttgccg
     1921 cagcgggatc cattgacgga tgcctatcgt ccgggcggcc gcgcgatcaa ctatcgtagc
     1981 gagccgttcg gcatcaataa catgcacgtg cagcacgaat acttcgggtt cgaagacgaa
     2041 tcaatgggct atagctccta tacgtttggt gatccgtccc cgacgattcc gcgttcgtac
     2101 atgggagatc ccgccaaatt ccggctggtt catggcggct cggaagtgtt ccacagccac
     2161 catccgcacg gcggcacgat tcgatggcca cgcagcccgc gggcaatcga cgacatgaat
     2221 ctgtgggcca cgagcacgaa cggtccggtg aagtatccgg tgattcgtgc caagacggac
     2281 cgcgtcgatg tggaagtgat cggaccgtca gaagctctcg atctcgagac cgagtgcggt
     2341 tccggcctgt gccaacagct ggcaggagac ttcctcttcc actgtcacgt cgcccatcat
     2401 tatgtggcag ggatgtgggg ctactggcgg gtgtacaaca cgattcaaca gggcgatatg
     2461 cggaatgacg tgatgccgga cttgcgtgag ctgccggatc gcaaagggcg catcaagacc
     2521 ccaatctcat ccgacaaatt gatcggccaa acggtcgatt ggttcggcac gcagttcaag
     2581 atcatcgaca aggggaagag caactggaaa ggcaatccgg ccacaatcac catcaaggac
     2641 tgggtcgaga tgcagatgcc cacgccgggc aagcctggtc acaaggacga cgagaagggc
     2701 cagattgtct cctatgacgc cacggtgctg gactggacct ggaacggcaa tgtggcgatg
     2761 agcgaaaagg aaagcacgat cgacaatccg aagtacaaat ccacccatcc gggcaagcgg
     2821 catccgatca tgttcgagcc gctgaccggt aaagtggctt ggccccacct gacaccgcac
     2881 ttcggcaagc gtgtcatgtt ctctccgaac cacgttggcg cgccctggtt ggaaatgatc
     2941 aggcgcgatg cgaatggcga agaaagcgtc gatcaggccg cgcccggcga gcagggcaac
     3001 tggagcctct gtcccgtcaa tgcggggcgt aagaactaca acgtgcattt catcaagctg
     3061 ccgatcaaaa tcgcaaaggc gcagggcaag gaaccgcccg tagtcgatcc gaacggcttg
     3121 atctacgtgc tccatgaaga agaagccgct atccgggcca atgacgactt gaaatatccg
     3181 ttggtcgtcc gcggcaacat ctatgactgt gtggactgga tgttgaccag cgaatgggat
     3241 gacgacgatt acacgaattt ccagtcatcc aagattaaca cccactggca tttcctccag
     3301 ttcgacaatc aggcctccga cggcgtcatc accggcttct cctatgaaca gtcggtgcga
     3361 ccgttcacca tgctcgagaa gaagaacaag aagggcctgc cgatgccgat gaacaccgcg
     3421 ctgacggcgg cggcgaagaa gggctcgaac agtctgtcag taaagaatgc gaagcaatac
     3481 cacgtcggta ccttgattct ggtcggagcc gacaacgtga aggggaatga gatctcgcgt
     3541 atcaaggcga tcaacggcaa tacgatcacc ttggcgaagg gattgaagaa cgatcacccg
     3601 gcgaacgaca tcgtgacggt ggaattcgtc cggcagcggt tctgggtcga tgcggacgtc
     3661 ggtacggtct tctggcacga ccatgcgttc ggggccacga catggccgca cggcgggttc
     3721 ggcacgttta tcgccgagcc ggtcgggtcg acctatcacg atccgaaaac cggcaagctc
     3781 gtccggagcg ggcctattgc ggatatccac accaatgagc cggtcggtca tggcgtgaac
     3841 aacagcttcc gtgaattgat ggtgcaggtg cacgacactg tgccgcacac cgtcaacatc
     3901 gtgaccgcgg gcaatccgcc ggggcagccg gttgaggtgg cactggaagc cggcaagacc
     3961 gtctccttca tgatgccgga aaagctgtac atgaccccga tgccgttcct gaatggaggg
     4021 acgcacacga caggcagcgg tctcaacttt agagccggcc caatcgcgca acgattggcg
     4081 accaatccgg atgtctccca ggcgttcaac agtcaaattc acggagaccc gtatacgccg
     4141 cttctgcgtg catacacggg cgatacgatg gtgttccggc tcttgcacac gctcatgaac
     4201 gagacgatga cctggacatt ggccgggcat acgttctggt ctgagcgcta tgcgtccgat
     4261 gcgaatcgca agaattcgat ccacatcgga attgccgagc gctacgacct ggtcgtgccc
     4321 gaagcgggcg gaccgcggca tcaagcgggg gactacattc acttcaatgg acgttcctcg
     4381 aagttttccg agggcggctg gggcatcatt cgggtgtacg acaaggagca ggccgatcta
     4441 aagaagcttc cggccggttt ctcggtgaag ggcgaaatcc cgaaagcctt accagtctgt
     4501 ccggcggacg ccccggtgaa gaccttcaac gtcgtggcga tggattatcc tgccatgacc
     4561 ttcaatgcga aggcgccgga agcaatcgaa gtggactttg agcggaagat ccaaattacg
     4621 aatccagacg ccaagatcta cgcactggag gaagatgccg cgaaggtctc gggcggatcg
     4681 catcccatgc cgctcaccct ccgggtgaac gtcggggatt gcgtgaaagt gaagttgaaa
     4741 aacaagatga aagagggcaa ggcgtcgttc tcggccatcg ggtt
//
LOCUS       DASTQY010000185         5970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_37649, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000185 DASTQY010000000
VERSION     DASTQY010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37649"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VFR82_06110"
     CDS             <1..674
                     /locus_tag="VFR82_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEU4501209.1"
                     /translation="IDNIIFISVLVGRLPEAQRAVARRLGLGLAMVARLGLLLSISWI
                     MGLTQPWFALLGNEISGRDVILIGGGLFLLAKATHEIHYSLEGIGEKDTEVLGSSFGM
                     AMVQISLLDIVFSLDSVITAVGLVEEVSVMAIAIILAVLVMMIAARSIGTFVDEHPTI
                     KILALSFLILVGVTLMMEGFDVHVPKGYIYFAMAFSVAVEMLNIRMRKKRSIPVKLHS
                     KYSAG"
     gene            complement(922..1296)
                     /locus_tag="VFR82_06115"
     CDS             complement(922..1296)
                     /locus_tag="VFR82_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4501210.1"
                     /translation="MAVTRVLLVDDESLVRRTLKQILSAYQDLELVGEAANGEEAISA
                     MERLQPDVVVMDIRMPAFDGIAAVRAIREKYPRAKIIGLSEYAQSYNIDAMERAGALG
                     VYLKSMALEELYPAIKAAHPAI"
     gene            complement(1332..1748)
                     /locus_tag="VFR82_06120"
     CDS             complement(1332..1748)
                     /locus_tag="VFR82_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4501211.1"
                     /translation="MTATLAPLVHRVLLVDDYVPVRIILRNMLTGYVDLQIVGEACDG
                     KQAIEQVAACQPDIILMDYNMPLMNGIEAARLIKKSWGETVIIGLCTVQDTYIIDGFL
                     EAGALAVIFKDRIDHLHSTIQRAFPRASSDRLVVQH"
     gene            2844..3233
                     /locus_tag="VFR82_06125"
     CDS             2844..3233
                     /locus_tag="VFR82_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4501212.1"
                     /translation="METLRVLLVEDDAMLRQTLARMLAAYPEVQVIAQAATGDEAVSY
                     VEKHNPHVVVMDIRLPRMDGIAATRVIKWKFPQVQIIGLSEYGHGYNANAMLKAGAIA
                     VYQKSMATEELYSAIKKAGRGFSQLSA"
     gene            3388..3576
                     /locus_tag="VFR82_06130"
     CDS             3388..3576
                     /locus_tag="VFR82_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501213.1"
                     /translation="MYRNPYVLKQFGDSCTQLAQQLKNETFTADDRMFVENHLLIVQL
                     ALAMSKYAHLRAQANGDE"
     gene            3573..4169
                     /locus_tag="VFR82_06135"
     CDS             3573..4169
                     /locus_tag="VFR82_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4501214.1"
                     /translation="MRHALIVDDHPVVRDGVRDILQRTFPSIQIKVSSGGAGLLEEVC
                     GTGWAFIVLDINLPGQNGLNIIRQAKACCSTIPILVFSLYSERQYAARALRAGAVAYI
                     SKDRPPVELIETVTAILEGKPLNKPRELMVSQPLLSDREVQVLGLLVRGLDRKEIAQE
                     LKINEKTVSTYRARLLQKLEAKNTVELIRYALDEGLVD"
     gene            complement(4292..5182)
                     /locus_tag="VFR82_06140"
     CDS             complement(4292..5182)
                     /locus_tag="VFR82_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEU4501215.1"
                     /translation="MQRLAIVEAPSILGLKPTGVETLPYTLLANGLAEQLNARHAARV
                     EAPPYSADRDPVTLTLNDRSINHYSTRLADAICSVLDNGEFPVVLGGDCSILLGSMLA
                     LRRHGRYGLLFIDGHADFYQPEVNPNGEVASMDLAFVTGRGPAILTDIEGRRPLVRDE
                     DCVVFAFRDAEEQARYGSQALPPEMLALDLARVRDRGAETSAREAVRHLTREHGASAG
                     FWIHLDADVLDDAIMPAVDYRLEGGLSWAELATVLRIAADSKRAVGIEVTIYNPKLDP
                     AGASGKELTSTLVKGLSPLH"
BASE COUNT         1410 a         1552 c         1593 g         1415 t
ORIGIN      
        1 gaatagacaa cattatcttc atctccgtcc tggtcgggag gctgccagag gctcagcggg
       61 ctgttgcgag aagattgggc cttggcttgg cgatggtggc tcgtctgggg ttgttgttgt
      121 cgatctcctg gattatgggc ttaactcaac cgtggtttgc actgcttggc aatgagattt
      181 ccgggcgcga tgtgattctc atcggaggtg ggctattcct cctcgcgaag gccacgcatg
      241 aaatccatta cagcctagag ggaatcggag agaaggatac tgaagtactt ggaagcagct
      301 ttgggatggc aatggtccaa atctcgctcc tggacatcgt cttttcgcta gattccgtga
      361 tcacggcggt cggattggtt gaggaggtat ctgtcatggc catcgccatt atccttgcgg
      421 ttcttgtcat gatgattgcg gcgaggtcca tcggaacttt cgttgatgag catccaacga
      481 tcaaaattct cgccttgtcg tttctgatcc tcgtgggagt cacgctgatg atggaaggat
      541 tcgatgtgca tgtgccaaag ggctatatct attttgccat ggcgttttca gttgcagttg
      601 agatgctgaa catccgtatg cgtaaaaaac ggtcgatccc ggtcaaactg catagtaaat
      661 actcggcagg ttgaccgccg gcagtgccct agcgtcacat ctgtccttca gccaggcgga
      721 gtacattcaa gagcaggccg ggtagagcgc catccaggtc atgatgtata catacgggca
      781 cgtgttcccg agcggaaacc gaggaggggt gtgtaagctc gctctgacgg tggtaacggg
      841 caaatccgca atccagctgc acccgcaggt ggtcgcggcc gaataaaagg tccggaatat
      901 ttggccataa aagtcggcgg cctaaatcgc cgggtgagcg gctttaattg ctgggtacaa
      961 ttcttccaag gccatcgatt tgagatagac ccctagagcc ccggctcgtt ccatcgcatc
     1021 gatgttgtaa ctttgcgcat actctgacaa gccgataatt ttcgcacggg gatacttttc
     1081 tctaattgca cgaaccgcgg cgatcccatc gaatgcgggc atgcgaatat ccatcaccac
     1141 tacgtcaggt tgaagcctct ccatggcgga aatggcttct tctccattgg ctgcctcacc
     1201 aaccaattcc aaatcttggt aggctgaaag aatttgtttg agcgtcctgc ggaccaggga
     1261 ttcatcgtct acgagtaaca cgcgcgtcac tgccatagtt cacaccccga tcttttgtgg
     1321 cattgcgaac cttaatgttg cacgacaagg cgatctgatg acgctctggg aaaagctcgt
     1381 tgaatcgtgg aatgaaggtg atctatccgg tctttaaata tcactgctaa ggcgcctgcc
     1441 tcgagaaatc catcgatgat gtaagtatcc tgaaccgtac agagtccaat gatgactgtc
     1501 tctccccagg atttcttgat aaggcgagca gcttcgatgc cgttcatcag tggcatgttg
     1561 taatccataa gaataatatc gggctgacag gctgcgactt gttctatagc ctgcttacca
     1621 tcacacgctt ctcccacgat ttgcagatca acataccctg tgagcatatt ccgcaaaatg
     1681 attcggaccg gcacgtaatc atcgaccaat agaacgcgat gtacgagagg tgccagggtt
     1741 gctgtcatag tccaccccat ctaagaaact accaaaacat aacatagctg atcattttgt
     1801 tttactagcc agatcgatag gttacgagtg cctacagccg tgtaagccgt agtcgaaatc
     1861 gcacgtattt ccagtgaagt agcctcagca ttggaaattg atgcagccgg agaattatgc
     1921 cggtcggaag aacgtgttta aacacatcaa agcaaaagca ggaaaccctt gtccatttaa
     1981 cgaactgaga aggtgaacac ctgataggtc acgaccccaa tcaatagggc ggcgagctca
     2041 attcgacacg gtgcagtatg ggagaaaaac gaggtgacga ggaatccgtg agtgattggt
     2101 gattcttcgc tacgacgtgg tgtgccgcgc gttcctaatg cttctggtgc gagttttcac
     2161 tgaaatgtag cagggtagac cgcggcgtgc tatggctatt acgaagcgag ggagggacac
     2221 cagaaatcgc ttaccgttac cagcaaatca gagaagccca atgaacaaag gcattatcct
     2281 cctcaccttg atcatcctct ttccagtacc ggggtgggcg ggtctctctc aagcccataa
     2341 aacatatgaa ccgcgtgatt acacaaccgc agctaaagag ttttggccct tacgtagcga
     2401 ccttgaacgt gccgttgata gttgcactca atcggtccat cgagacgatc ctgacttcga
     2461 cgcatacaac gaaggctcgt ccgtcgtcct gtttggtgcg acgccagagt cacgatttgc
     2521 atttcacaaa tgcatgtcca ggaaagggta tcccctgacg aaaacaggaa aataagtgtt
     2581 ggtctcgtct ccggccccgt tctacatcta ctgatttcga tctcactgac ccgacttcca
     2641 gaagcaaatc tcctgaaaga gttcgagttg ccgcgttgtt gtcttccttt tctctgtagg
     2701 ctcgcgccta cctattgtaa gggcacgtaa cctcacaatt tcatgacaga ttggacgtca
     2761 tctatgttac attcgacggc aatgtcgtct aggatacatt tccctcagat cggttcttcc
     2821 ttgtttttcc gagggtcgag atgatggaaa ctcttcgcgt gctcctcgta gaagatgacg
     2881 caatgctccg tcagactctg gcgcgaatgc ttgcagccta ccccgaggtg caagtcattg
     2941 cgcaggcagc aaccggtgac gaagcggttt cctacgtcga aaagcacaac cctcacgtcg
     3001 tggtcatgga tattcgcttg cccagaatgg atgggatcgc ggcgactcgg gtgattaaat
     3061 ggaaatttcc gcaggtgcag attattggtt tgtctgaata tggacatgga tacaatgcaa
     3121 acgcgatgct aaaagctgga gccattgctg tgtaccagaa atccatggcc actgaagaac
     3181 tgtattcggc catcaaaaag gccgggcgag gtttctcgca gttatcagct tgagtgaacg
     3241 tcgaagccgt ctgggactcg gcagcgtaca agcgcccttc tagtgcaacg tccagtgtca
     3301 taaatgcaca gccgtgtagg tcgtcgctga tagagcgact tctcctcttt gtgtagactc
     3361 gcgcagattt gtcgtttaat tggagttatg taccgaaatc catacgtcct taaacagttt
     3421 ggtgattcct gcacgcagct ggctcagcag ctaaaaaacg agacgtttac ggctgacgat
     3481 aggatgtttg tcgaaaatca tctgctgatc gttcagttgg cgctggccat gtcgaaatat
     3541 gctcatttga gggcgcaggc caacggagac gaatgagaca cgctctgatc gttgacgacc
     3601 atccggttgt gcgtgatgga gtcagagaca tactgcaaag aacctttccg tcaatacaaa
     3661 taaaggtgtc atcgggtggc gctggtctcc tggaagaagt atgcggtact ggttgggcat
     3721 tcattgtgct ggacatcaat ttgcctgggc aaaatggact caacatcatc cgacaggcca
     3781 aagcctgctg ttcaactatc ccgattctcg ttttcagcct ttactctgaa cgacagtacg
     3841 ctgcccgtgc cttacgggca ggagccgttg cttatatttc gaaagatcgt cctcccgtcg
     3901 agttaattga aactgtgacg gccatactgg aagggaaacc actcaacaaa cctcgggagc
     3961 tcatggtctc acagccgctc ttgtcagacc gtgaagtgca ggtacttggc ttacttgtaa
     4021 gaggtttgga tcgaaaggaa attgctcagg aactgaagat caacgaaaaa accgtcagca
     4081 cgtatcgagc gagactactt cagaagctag aggccaagaa tacggtcgag ttaatacgct
     4141 atgcgctgga tgagggttta gtggattagg actggtcctt cttcttttaa gggcgtgatt
     4201 ttaaccgcat tggggacccg tggcaaactc actatcacga acaaccgcag caacggctta
     4261 cgagacgcgg gaaaaatgca cactcgcaga ctcagtgaag tggggagagt ccttttacca
     4321 gtgtcgaagt gagctctttt ccgctcgcgc cggccgggtc aagttttgga ttgtagatag
     4381 tcacttcaat acccactgcc cgctttgagt cggcggcaat gcgcagcacg gtggccagct
     4441 ccgcccacga caatccaccc tccaagcggt agtccaccgc cggcatgatg gcgtcgtcga
     4501 gtacatccgc gtcgaggtga atccagaaac cggcactcgc gccgtgctca cgcgtgagat
     4561 gtctcaccgc ctcccgcgcg ctggtttccg cgccacggtc gcggacccgt gcgaggtcga
     4621 gcgcgagcat ctccggaggc agcgcctggc tgccgtatct ggcctgttcc tcggcatcgc
     4681 ggaatgcgaa taccacacag tcttcatcac gaacgagagg gcggcgtccc tctatatcgg
     4741 tgagaatcgc gggaccacga ccggttacga acgccagatc cattgaggcc acttccccat
     4801 tcggattgac ttcgggttga tagaagtcgg catgtccatc gataaacagg agaccgtaac
     4861 gtccgtgccg gcgcaatgcc agcatactac ccaacaatat cgagcaatca ccacccagca
     4921 ccaccggaaa ctctccattg tcgagcacgg agcaaatggc atcggccagg cgagtcgaat
     4981 aatgattgat cgaccgatcg ttgagggtca gcgtaacggg atcgcgatcc gcgctatacg
     5041 gcggagcctc gacgcgcgca gcgtggcgag cgttcagctg ttcggccagt ccattggcca
     5101 gcaacgtata cgggagcgtt tcgaccccgg taggcttcag gccgagaatc gacggtgcct
     5161 caacgatcgc aagtctttgc atcctgtgcg cttccctgtg gcccgtgttt tggtgcgatc
     5221 gcgttagaca aagctgcgac gaatgttcgc agtgtcgatc catcgcttag gccgtgatcc
     5281 ggattcagct cgcatacggt aagccaccga aagctctccg tatcacccaa gtgtgtacgt
     5341 caatgcccac atcgcggagg cgctccagca acccggcagc gcgcagcgcc gtcggagcac
     5401 gttcctgccc cggtgcgtag gcgccggcac tactcggtgc accgacgaca gagaggcgag
     5461 gcctgctctc tgaacgtttc tcgccaggtg cgtgtccgtc cattgcacgt tctctggtgt
     5521 ggtgatcaag ggatcagacg atggcgcggt cactcatccc acccggcaga tagccggatg
     5581 atcaggcagc taaccgtgcc tcaaacgatt ctaatgggcg cgtccgatac tcggcatacc
     5641 gcacgtcgta caggcacaca gaaaaacacg ccactcagat ctgcccgttg cgtagacaga
     5701 tcgaccggct gttccttatg acttgtggca gcgtggacag atgcagtatg tgttcgggtg
     5761 gtatccattc gagtacgata ttgccgctct tgttgcctag cgactgggat aatcccgagc
     5821 cacgatgaca atataagtac ctaattaata taattaagat gcgatgctac ggggtctttc
     5881 tttcgatgcg cttgatgcat ttatggccga gcagaactgc gtgtactcgt acgtcatggc
     5941 ctacaaagct gtaggtaaac taacttatct
//
LOCUS       DASTQY010000186         3761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1445002, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000186 DASTQY010000000
VERSION     DASTQY010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445002"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VFR82_06145"
     CDS             <1..523
                     /locus_tag="VFR82_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU4501216.1"
                     /translation="NFQDANKKAVKYWRDIGTLDAYWEANLDLVAVDPLFNLYDQQWP
                     IRTYQGQFPPAKFVFAQDYQGGRMGVALDSIVCGGCIISGGRVQTSVLSPNVRVQDHA
                     DVRESVIMENVVIGEHCRIKRAIIDKDVDIPPKTEIGYDRAADAQRFHVTDSGIVVIS
                     KGMKLNASLDSSG"
     gene            498..2354
                     /locus_tag="VFR82_06150"
     CDS             498..2354
                     /locus_tag="VFR82_06150"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEU4501217.1"
                     /translation="MPPSIHPVDLIIALRQKHLTPAIVFLTSRRACDEAMESFEHTQS
                     FLPPARQEMIGKVLRAVIDQYPSIAEHPLIPTVQRIGVAAHHAGHLPSWKIAVEELMR
                     SGCLDAVFATTTLAAGVDFPARTVVITQSSIRKARDFTDLTIGEVQQIAGRAGRRGKD
                     LVGFAVVTPSPYIDLTVLTKGFTGHPEAINSQFVISYPMVLNLLKAHPLDQIQPILAK
                     SFAQFQLNQRAEVLERKLDELHRQLEPYGPRVCTDWISQWQLFDHVRRQRAHRTPVRR
                     HEPPEVAARFHFLTPGRVAGLSKSRGVVLRQYRSKGQKSPMVTLLRPGGAITECPAAI
                     VTEVFDHMLDCAETPVYPWCTAGSIESMLRDLEELPPRLPVLPILVPKEELIPDSIVQ
                     TLGDFSCPTCPSRSACQKDHAHASKLRQEQHRHMKAIQALRTSLWHRFQEKIDALQRF
                     GYLSPTAHLTDTGEWARLIRIDHSLLITELVRADAFTGASPALLAAVMSSIAHDDDRP
                     GAFPRISTGLASLLSQVRKLAESLSAYEDPPLLRADIAAVTEQWIGDPTLTWIGLCKK
                     TTMAEGDIYRLLARTLEFLSQLHTLRDTHPALADCASQAIANLRRGVLEELP"
     gene            2437..3501
                     /locus_tag="VFR82_06155"
     CDS             2437..3501
                     /locus_tag="VFR82_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4501218.1"
                     /translation="MTTDELERLLVQQPLGVLHRLARGRVHRHFRAGKRRLIELLLRH
                     SAENRAGFESDLLALISERETGPAIQPARHQLSERPKAAQPGRRLHSAQHEPDHHEPA
                     ASLAAWLEGIGVPPPQPFVPDPWQEEALAKLQTGDVIVSVPTGSGKTYVAVEAARRAM
                     DENRTVIYTSPLKALSNTKYTEFCRIFGAERVGILTGDRRDNGQAPLLIMTTEILRNL
                     LYDAAGGEIDVRLDTLGLVIMDESQYLADPERGVVWEETIIFCPTQARLLLLSASIGN
                     PHDIADWLTSIRSTPCHLIKHSKRTVPLRAGYLHPDGKLTPLFRTIGIPNGHPVHLHP
                     EAKRLFARYEDETMPSGRRR"
BASE COUNT          794 a         1144 c         1055 g          768 t
ORIGIN      
        1 taactttcaa gatgccaata aaaaggccgt caaatactgg agagatatcg gaacgctcga
       61 cgcctactgg gaggccaatc tcgatctcgt ggcggtggac cccctattca acctctacga
      121 ccagcaatgg cccattcgca cctaccaggg acagtttccg ccggcgaagt ttgtcttcgc
      181 ccaggattac cagggagggc gcatgggggt ggcgttagac tcaatcgtct gcggaggctg
      241 cattatttca ggagggcgag tgcaaaccag cgtgttgtcg cctaacgtgc gtgtccagga
      301 ccacgcggac gtgcgagaat ccgtgattat ggagaatgtg gtgatcggtg aacactgccg
      361 gatcaagcgc gccatcatcg acaaggacgt ggacataccg cctaagaccg agatcggcta
      421 cgaccgcgcg gccgatgccc aacgcttcca tgtgaccgac tccgggatcg tggtcatttc
      481 aaaaggtatg aaactgaatg cctccctcga ttcatccggt tgatctgatc atcgccctac
      541 gacagaaaca cctcaccccc gccatcgtgt tcctgacgtc gagacgcgcc tgcgacgagg
      601 cgatggagtc cttcgaacac acgcagtcct ttttgccgcc ggcgcggcag gagatgatcg
      661 gaaaggtcct gagagccgtc atcgaccaat atccgagcat tgccgaacat cctttgattc
      721 ccacggtcca gcggatcggt gtcgccgcac atcatgccgg gcacctgccc tcctggaaga
      781 ttgccgtcga agaattgatg cggagcggct gtctggatgc ggtcttcgcc accaccacgc
      841 tggcggccgg tgttgatttt cctgcccgga cggtcgtgat cacgcaatcc agcattcgca
      901 aggcgaggga cttcaccgat ctgactattg gagaggtaca gcagatcgcc ggacgggccg
      961 gccgtagggg aaaagacttg gtcggctttg ccgtcgtcac gccgtcacct tatatcgacc
     1021 tgaccgtcct taccaaaggg ttcacgggcc acccggaagc tatcaacagc cagtttgtca
     1081 ttagctatcc tatggtattg aatctcctga aggcccatcc gctcgatcag attcagccga
     1141 tccttgccaa gagcttcgcg cagttccaac tgaaccaacg agccgaggtg ctcgaacgga
     1201 agctcgacga gttgcaccgg cagttggagc cttacggccc ccgtgtctgc actgactgga
     1261 tctcgcagtg gcagctattc gatcatgtgc gccggcagcg cgctcatcgc acgccggtca
     1321 gaaggcacga accgccggag gttgccgcac gttttcattt cctgacgcct ggacgcgtcg
     1381 caggcctctc caagagccgg ggtgtggtgc tccgacaata tcggagcaag gggcagaaaa
     1441 gtcctatggt cacgctgctg cgtcccggcg gcgccatcac cgaatgtcct gctgcgatcg
     1501 tcaccgaggt attcgaccac atgctggatt gcgccgagac tccggtctac ccgtggtgca
     1561 cagctgggag catcgaatcc atgcttcgcg atctggaaga gctgccgccg cgcctcccgg
     1621 tgcttcccat tctggtacca aaagaagagc tgattccgga ttcaatcgtg caaacattgg
     1681 gcgatttctc ctgcccgacc tgtccttccc gatcggcctg ccagaaggat catgcccacg
     1741 cgtcaaaact gcgtcaggaa cagcaccgtc atatgaaagc gattcaagcg ctccggacca
     1801 gcctgtggca tcgttttcag gaaaaaatcg atgcattgca gcgttttggt tatctgtcgc
     1861 ctactgccca cttgaccgac accggtgaat gggccaggct cattcgcatc gaccattcgc
     1921 ttttgattac cgaactcgtc cgggctgacg ccttcaccgg tgcctctccg gccctcttgg
     1981 ctgccgtcat gtcgagcatc gcgcacgacg atgaccggcc cggggcattc ccccgcatca
     2041 gtaccgggct cgcctcgttg ctctcacagg tgcgaaaact ggccgagtcg ctctcggctt
     2101 atgaagaccc tccgcttctc agggcggaca ttgccgcggt taccgagcaa tggatcggcg
     2161 acccgaccct cacgtggatc ggtctctgca agaagaccac gatggccgag ggcgatattt
     2221 atcgactgct tgcacgaacg ctcgagtttc tttcacaact ccacaccctg agggacacgc
     2281 acccggcgtt agctgattgc gcatcgcaag cgattgccaa tttgcgccga ggagtcctgg
     2341 aggagttgcc gtgacgcgaa tggatgtact ggaaaatgta ggcaccgcgc gttttgttgt
     2401 tcgggccttc gatctgccga aatccacact gaagtcatga ccaccgacga actcgaacgt
     2461 ctccttgtcc aacagccgct cggcgttctg caccgtcttg cccgcggcag ggtgcaccgc
     2521 cattttcgcg ctgggaaacg ccgactgatt gagctcttgc ttcgtcattc ggctgaaaac
     2581 cgggcaggct ttgaatcgga cttgctggcg ctcattagcg agcgtgaaac ggggcctgca
     2641 atccaacctg ctcggcacca actatccgaa cgcccaaagg cagcacagcc cgggcgtcgg
     2701 ctccattcag cgcagcatga gccggatcat cacgaacccg cggcttcgtt agccgcctgg
     2761 ctcgaaggaa tcggcgtccc tccacctcaa ccgttcgttc ccgacccctg gcaggaggag
     2821 gcgctggcca agctgcaaac cggagacgtg atcgtcagcg tgccgacagg cagcgggaaa
     2881 acgtatgttg cggttgaagc ggcaaggcgt gccatggacg aaaaccgcac cgtcatttac
     2941 acatcaccgc taaaggccct ttcgaatacc aagtacactg aattttgccg catcttcgga
     3001 gcagaacgcg tcggcatctt gacgggcgac cgtcgcgata acgggcaggc tccgctcttg
     3061 atcatgacca ctgaaattct gcgcaactta ctctacgacg cggcgggtgg cgagatcgac
     3121 gtgcggctcg atacgctcgg attggtcatc atggatgaat cgcaatatct agccgacccg
     3181 gaacgcggag tcgtctggga agagaccatc atcttttgtc cgacgcaggc gagactgttg
     3241 cttctctccg cctcaatcgg taatccgcac gacattgcgg actggctcac gtccatccgt
     3301 tccacgcctt gccacttgat taagcacagt aaacggacgg tgccgttgcg tgccgggtat
     3361 ctgcacccag atggcaagct tacgccactc ttccgcacca tcgggatacc caacggccat
     3421 ccggtgcatt tgcatcctga agccaaacga ctcttcgccc gctacgaaga cgaaacgatg
     3481 ccgtctggca ggcgccgctg agtagctacc accaacttcc agtcgagaat atactcgatg
     3541 tgcctctttg aatggagcgt gagggaaggt gactctgcct gagtcaatgc cagaaaagct
     3601 ggagttgggt tagaaggagt agctaatgcc gaacgtcacg ccgtgacgga agctttgaag
     3661 ctccgtgagg ggaaactcgt ctgagccgcc aattgcattg tgaaaggtga cggttccgtc
     3721 gacagtgcgg ttccaccaga ggcgataacc gacattcgca t
//
LOCUS       DASTQY010000187        12244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1241804, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000187 DASTQY010000000
VERSION     DASTQY010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 12244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12244
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241804"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="VFR82_06160"
     CDS             complement(<1..1427)
                     /locus_tag="VFR82_06160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU4501219.1"
                     /translation="MALIQPMRQGQLLKPARGKRLLLVDPYPRNSQYHLTASERRAVW
                     FPKLSLPTIAAYTPDSWDVDLVDEAVQDIDFDYPCDMVGLSIMTCYAPRAYEIAKEFR
                     KRGKTVVMGGVHPTYCPDEALQHADAIVCGEAEDLWPRLVADYEAGMMQRVYKMTNFP
                     SLEHYKAPRVELLSPDSYMTRQCSFTTRGCHFDCEFCSVSPFNGKTTRRRPVPEVIAE
                     LQNVQRWIRSELVERIRDEPLWNAFLAALKIRIGLDDGSIVAFVDDLHNSNRAYCREL
                     WTALKPLKIKWGCQSTLFLGDDEEMVKLAAESGCVSVFVGMESLDEDCLDETNKPFNR
                     VQKFSQEIKMFHKYGIMVNPGIVFGFDNDDEAVFERAVEFLKKNQVELAYFNVLTPLP
                     GTALFDRYNNAGRIFDRDWSKYDGKHVVFQPSRMTPEQLQEGFYWANHQFYSLPNIWY
                     RLSGTKQRFIARWEMNREFRKLVKR"
     gene            complement(1703..3109)
                     /gene="argH"
                     /locus_tag="VFR82_06165"
     CDS             complement(1703..3109)
                     /gene="argH"
                     /locus_tag="VFR82_06165"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU4501220.1"
                     /translation="MTKERRANGLANGKAWSGRFREPTHRLVETFTSSVAVDRRLYAE
                     DIQGSIAHCKTLEKARVLTQTESRTIVRALEAVKLELDRGKFRFVPHDEDIHMAVERR
                     LTELIGPLGGKLHTGRSRNDQVALDVRLYLRHQLGLFAERIADCQRVLVAKAKANRAV
                     AMPGYTHLQRAQPVLFAHHLLAYVDMFERDKGRIQDAQKRLNVMPLGSGALAGTNYPV
                     DRRYTAGLLGFPRVTTNSLDAVSDRDFMIEIAAALSITMMHLSRMSEELILWSSQEFR
                     FVDLPDGFCTGSSMMPQKKNPDVPELVRGKTGRVYGQLMNLLTTMKALPLSYNRDLQE
                     DKPAVFDALDTVTASLEVLTELVRRLKVNREALKQAVQGGGLLATELADYLVNKGIPF
                     REAHAITGRVVRTALERGLELSSLSLQELRQFSERFDNGVFARLTVDAAIDRKAQIGG
                     TARGQVEQRIKELERMLS"
     gene            complement(3109..4320)
                     /locus_tag="VFR82_06170"
     CDS             complement(3109..4320)
                     /locus_tag="VFR82_06170"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU4501221.1"
                     /translation="MNRGIKKVVLAYSGGLDTSVILKWLQETYEAEVIAFCADLGQGE
                     DLQAVKAKAQKLGVKKIYIEDLREAFVREYVFPMLRGNAMYEGSYLLGTSIARPLIAR
                     RQAEIAMKEGAEAVAHGATGKGNDQVRFELTYMALAPRLKIIAPWREWKMRSRRELIE
                     YAERHGIPVTATKAKPYSMDLNLFHVSYEGGILEDPWEPPPEEIFQMTVSAEKAPSKP
                     VEVEIDYEAGNPVAVDGKKMTPAALLGHLNKLGGAHGIGRIDLVENRYVGMKSRGVYE
                     TPGGTILHVAHRGLESLTMDREVLHFRDSLIPRFADLIYNGYWFSPEREMVQKAIDEA
                     QQDVTGTARVKLYKGSCTLTGRKSKHSLYRLDIATFEEDEVYNQKDAEGFIRLNALRL
                     KIRAQRKKASS"
     gene            complement(4323..5288)
                     /gene="argF"
                     /locus_tag="VFR82_06175"
     CDS             complement(4323..5288)
                     /gene="argF"
                     /locus_tag="VFR82_06175"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU4501222.1"
                     /translation="MTRASERRPKPVKDLLDVAALSRAEIEGLLTLATVLKAKQQRGT
                     SHRLLQGKTLGLLFQKPSTRTRVSFEAGMNQLGGHALVLPMADIQLSRGESVADTARV
                     LSRYLDGIVIRTYDHATVEEWAREATMPVINGLTDLSHPCQALSDLLTIKEKKGRLRG
                     IKIAYVGDGNNVANSLIEAVAKMGMTIALGCPSGYQPDQHVVDVARIEAVKTGATIDV
                     AQDPYVAVKEADVVYADVWISMGREREQARRLKVLAPYQVNSRLVAKAKPDAIVMHCL
                     PAHRGEEITAEMLDGQQSVIIDQAENRLHMQKAILTKLLGKRRPG"
     gene            complement(5302..6513)
                     /locus_tag="VFR82_06180"
     CDS             complement(5302..6513)
                     /locus_tag="VFR82_06180"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEU4501223.1"
                     /translation="MPTEELKESAEQYLMGTYTRQPISIVRGRGTKVYDLEGREYVDF
                     VGGIAVNILGYGHPDLVHAIQKQAAQLLHTSNLYYTEPQVKLAQMLVDHSFADKVFFC
                     NSGAEANEAAIKLARRYSHDKYGAGRFEIITMKQSFHGRTMATLTATGQEKVQKGYEP
                     LLPGFSYVPFNHVAELKEAVTENTAAIMLEPIQGEGGVHVAGRDYLKAVRDLCTQKDI
                     LLIFDEVQTGMGRTGTLFAYEQLGVQPDVMTLAKGLGGGVPIGACLATMSAAAAFGPG
                     SHASTFGGNPLACAAALAVFRVLLDGRVLDHAHRMGEYLSKGLSECKDCHHLVRDVRG
                     LGLLQGMEIEIDTKAVVSDCLKRGILINATGDHVLRFVPPLIISQPEVDRLLDVLSQI
                     FDRHAAQAASH"
     gene            6744..7292
                     /locus_tag="VFR82_06185"
     CDS             6744..7292
                     /locus_tag="VFR82_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEU4501224.1"
                     /translation="MSSTVAPTAWSRTDHERWRDFIGPNADRYLEQFKKFGSAQSPRF
                     ALTWNWPAFLFISFLWFLYRKMYLYAAVYAIGPMVSTYLTGDMTSGMVWSVVAGATAN
                     YVYYWHCREHIAAIKQQAWADHSKQHLAMKEAGGVQPYVVWVGVVFYALFFLTMFKIV
                     QEGGLDADKVPTKAKPATMTSA"
     gene            complement(7325..8149)
                     /locus_tag="VFR82_06190"
     CDS             complement(7325..8149)
                     /locus_tag="VFR82_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501225.1"
                     /translation="MSTIPLPNGPWRAVEAWWVRLGEKLQVADRAQSFFKAAGADGLL
                     DRIAHVGGQVDYILFVLMRYWVPTVLPPAGRERLTKSDSDYWLESEHILKAAAARLRE
                     LKPLIELLTTPSPISEASPGQNISASPVAELELSNIVEGIAEAAGSYGGPDYTSIVKA
                     FDPVPLRQTQPFKHNKKHSAELWVVFLLREHFRSLGLGKDRYWSLVAPLCTAAGILSQ
                     GGQGYGPDELKSWWQKNWPRTYTQLEQKQESADSGGAAYEQDWSWFQAWMSWQRRR"
     gene            8277..8882
                     /locus_tag="VFR82_06195"
     CDS             8277..8882
                     /locus_tag="VFR82_06195"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEU4501226.1"
                     /translation="MGRLILLRHGESQWNLENRFTGWVDVPLSARGIQEAKNAGDKLR
                     PFRFDRAFTSVLQRANETLRLALEVIGQTTIPTEKDKALNERMYGELQGLNKAETAQK
                     YGEDQVKIWRRSYDVRPPGGESLKDTAERVLPYYEKAIKPHVLAGETILIAAHGNSLR
                     ALVMELERLSKEQVLELNIPTGAPLLYELSPNGTVTNHRYL"
     gene            8966..9829
                     /gene="ttcA"
                     /locus_tag="VFR82_06200"
     CDS             8966..9829
                     /gene="ttcA"
                     /locus_tag="VFR82_06200"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEU4501227.1"
                     /translation="MPQASSSHPPLSRLDDATEQLQTRLLRQVGQTINDYGLIEAGDK
                     VMVCVSGGKDSYGLLDLLLTLQRRAPIHFDLIAVSLDQKQPGYPAHVLPEYLRSAGVP
                     FHIEERDTYSIVKRLIPEGQTTCSLCSRLRRGHLYRIATERGATKIALGHHRDDILET
                     LLLNLLFTGKLKAMPPKLRSKDGRHLVIRPLAAVREADLIRYAELRAFPIIPCDLCGS
                     QENLKRKQAKAFLLEWERLAPGCTDSMFAALSNIAPSLLMDQRLFDFRVLRRDESAAG
                     DEDAWLDEGNG"
     gene            complement(9826..10362)
                     /gene="msrA"
                     /locus_tag="VFR82_06205"
     CDS             complement(9826..10362)
                     /gene="msrA"
                     /locus_tag="VFR82_06205"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU4501228.1"
                     /translation="MKEEVATLAGGCFWCLEAVYDQMRGVNSVESGYTGGHQESPTYE
                     AVCGGRTGHAEAVRISFDPAIVSYRELLEAFFVIHDPTTLNRQGHDIGTQYRSAVFYH
                     TPEQKRIAEDVIALLTNERVYPSPIVTEVVAAPAWYEAEPYHQEYFARNPFQGYCTAV
                     VGPKVMKFRTRFSSKLKT"
     gene            complement(10499..10849)
                     /locus_tag="VFR82_06210"
     CDS             complement(10499..10849)
                     /locus_tag="VFR82_06210"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4501229.1"
                     /translation="MPLRSQLVKKPRVKEKRAHPRLTAQFRSTFSGGQREGHGKTLDL
                     SDGGCRIETDCPVVPNGSFECRIYAPGLSWPLRIEEAQVRWVQGRTFGLQFVAMRPDE
                     RAKLKQVIADLKAG"
     gene            complement(10932..11519)
                     /locus_tag="VFR82_06215"
     CDS             complement(10932..11519)
                     /locus_tag="VFR82_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4501230.1"
                     /translation="MKWLQSEGGEMAGSTNGSGWHGQGVNPDFPGMSQGGLRNELSGF
                     SQNPSDEYLSHGGGIASVSPATGGMRHRAELSKEEQAAVEAGLKDVFFGFDQWTLSDS
                     GMEALNHNAKWLKEHPGAVLKVEGHCDERGTTDYNIVLGDKRSKSARNYLTEIGLSSK
                     QIGIVSYGKERPFCLEHDESCYQLNRRGHVLLKRK"
     gene            complement(11767..11913)
                     /locus_tag="VFR82_06220"
     CDS             complement(11767..11913)
                     /locus_tag="VFR82_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501231.1"
                     /translation="MNHVILHDVRAAFFLMMGLLMLAMICAVAALGTVMRPVRWADRR
                     SAQF"
BASE COUNT         2595 a         3654 c         3282 g         2713 t
ORIGIN      
        1 ctgcgtttca ctaacttgcg gaattcccgg ttcatttccc atcgtgcgat gaaccgctgt
       61 ttcgtgccag aaagccgata ccaaatgttc ggcagtgaat agaattggtg gttggcccag
      121 tagaagccct cttgaagttg ctcaggggtc atccggctgg gctgaaacac gacgtgtttg
      181 ccgtcgtact ttgaccaatc acggtcgaaa atccgaccgg cattgttata ccgatcgaac
      241 agtgccgtgc ctggcaacgg agtcagcaca ttaaagtagg cgagctccac ctggtttttc
      301 ttcagaaatt cgacggctcg ttcgaatacc gcctcgtcgt cattgtcgaa cccaaacacg
      361 ataccgggat tcaccatgat gccgtacttg tggaacatct taatttcctg ggaaaacttc
      421 tgtacccgat tgaacggctt attcgtctca tctaaacagt cttcgtcgag cgattccatt
      481 ccaacaaaca cagagacaca gccgctttcc gcagccagct tgaccatctc ttcgtcgtcc
      541 cccaaaaaca gggtggattg acagccccac ttgatcttga gcggcttcag agcggtccag
      601 agttcccggc agtacgcccg gttactgttg tgcaggtcgt cgacgaacgc gacgatgctt
      661 ccatcatcaa gtcctatccg gattttcaga gccgcgagaa acgcattcca gagcggttcg
      721 tcgcggatcc gttcaactaa ttcactacgg atccaacgtt gtacgttctg gagttccgcg
      781 atgacctcgg ggaccggccg gcgcctcgtc gtcttgccgt taaaggggga cacgctgcaa
      841 aactcacagt cgaagtggca gcctctcgtc gtaaagctgc attgtctggt catgtaggaa
      901 tccggactca ataattcgac acgcggggcc ttatagtgct cgagagaagg gaagttggtc
      961 atcttgtaca cgcgctgcat catccccgct tcataatcgg caacaaggcg aggccacaag
     1021 tcctcggcct caccgcagac aatggcatca gcatgctgca gggcttcgtc aggacaatag
     1081 gtgggatgga ctccgcccat cacgacggtt ttcccccgtt tccggaattc ctttgcgatt
     1141 tcgtaggctc gaggcgcata gcaggtcatg atggagaggc cgaccatgtc gcatggatag
     1201 tcaaaatcga tgtcttgaac ggcttcatcg accagatcca catcccagga atcgggggtg
     1261 taggccgcaa tggtcggcag gctcagcttc gggaaccaga cggctcgccg ttcggaggcc
     1321 gtcagatggt attggctatt gcgcggataa ggatcgacca acaggagccg cttgcctcgc
     1381 gcgggcttca gcaattgccc ttgtcgcatc ggttggatca gtgccatcgg tcgctcctcc
     1441 actcctgtta tgagttacaa aatgtgtgtt gcgtgaccct agcgcgtccc caccggccgc
     1501 aatggcggga gatctacatc ttgaccctgg ggttccatga gagtttgcga ttcctcagcg
     1561 tcaggctttt tgtccaacgc gtcctgtctc ttttggcgtt caatcaacgg cgccaaaccg
     1621 acgtcctcgg gaggaatcgg cgacccgatc gttccacaag ccactgagaa gtaggcggcg
     1681 catgcaagga cgaggacttt cattagctta acatgcgctc cagttctttt atccgctgct
     1741 ctacctggcc tctagccgtg cctccgattt gcgctttacg atcgatcgct gcgtccacgg
     1801 ttaaccgggc gaagacaccg ttgtcaaagc gctcggaaaa ctgtcgcaac tcttgcaagg
     1861 acaaagacga aagctccaga ccccgttcca gtgctgttcg tacgacgcgg ccggtaatag
     1921 cgtgagcttc gcggaatggg atgcctttgt ttactaggta atccgccagt tcggtggcca
     1981 ggaggccacc accttgaacc gcctgtttga gcgcctcacg gttcactttg agccgacgca
     2041 ccaattccgt caagacctcc agcgacgccg tgactgtatc caaggcatcg aacaccgccg
     2101 gcttatcctc ttgcagatct cgattataac tcaaggggag cgccttcatg gtcgtcaaga
     2161 gattcatcaa ttggccatag acgcggccgg tcttgccccg gaccagctca ggcacatccg
     2221 gattcttttt ctgcggcatc atgctgctgc ctgtgcaaaa accgtccggc agatcgacga
     2281 accgaaattc ctgggacgac caaaggatga gctcctcgct catgcgcgac agatgcatca
     2341 tcgtaatcga cagcgcagcg gcaatttcga tcatgaagtc ccggtcggat accgcatcca
     2401 ggctgttggt ggtcacgcgt ggaaacccta ggagcccggc cgtgtagcgc cggtcgaccg
     2461 gatagtttgt gcctgccagg gcgccggacc ccagcggcat cacgtttaac cgcttttgcg
     2521 catcctgaat ccgtccctta tcgcgttcaa acatgtcgac gtacgccagc agatgatggg
     2581 caaatagcac cggctgggcc cgttgaaggt gcgtgtatcc gggcatagcc accgcccggt
     2641 tggccttggc cttggccaca aggacacgct ggcagtctgc aatccgttct gcgaaaagcc
     2701 caagctgatg acggagatag agtcgaacgt ccaacgccac ttggtcgttg cgactgcggc
     2761 ccgtatggag tttacctccc aatggtccga tcagctctgt gagccgcctc tcgaccgcca
     2821 tgtgaatgtc ctcgtcatgg ggtacaaacc tgaattttcc ccgatccaac tcgagcttga
     2881 ccgcctcgag ggcacgcacg atcgtgcggc tctccgtctg agtcaacact cgtgccttct
     2941 ctaacgtctt gcaatgggcg atgctgccct gaatatcctc tgcgtacagc cggcgatcca
     3001 cggcgaccga gctcgtgaac gtttcgacca agcggtgagt cggttcgcga aatcgcccgc
     3061 tccaagcctt gccgttggcc agtccgttcg ctcgccgctc tttggtcatc aggacgaggc
     3121 ctttttgcgt tgcgctcgga tcttcagccg caaggcattg aggcggataa agccttcggc
     3181 atctttctgg ttgtacacct catcctcttc gaaggtcgca atgtcgagac gatacagtga
     3241 atgctttgat ttccgtcccg tcaaggtgca actaccttta tacagcttca cgcgagccgt
     3301 gccggtgacg tcctgttgtg cctcgtcgat cgccttctgc accatctcac gctcagggct
     3361 gaaccaataa ccgttgtaaa tcagatccgc aaaacgcggg atcaggctgt cacgaaaatg
     3421 caggacctct cggtccatcg tcagggattc cagcccgcga tgcgccacgt gtaggatcgt
     3481 gcctcccggc gtctcataga cgccgcgtga tttcatgccg acgtaacggt tttcaaccaa
     3541 atcgatacgg ccgatcccat gcgcaccgcc cagcttgttg agatggccca gcaacgcagc
     3601 cggagtcatc tttttcccgt ccacagcaac aggatttccg gcctcataat caatctccac
     3661 ttccaccggc ttactcggcg ccttctccgc cgagaccgtc atctggaaga tttcctctgg
     3721 tggtggctcc cagggatcct caaggatgcc gccctcatag ctcacgtgga acaaattgag
     3781 gtccatgcta tacggcttgg ccttcgtcgc cgtaaccggt atcccgtggc gctcggcgta
     3841 ctcgatcaac tcgcggcggg aacgcatctt ccactcgcgc cagggagcaa tgattttcaa
     3901 gcggggggcc agcgccatat acgtcaattc aaagcggacc tggtcgtttc cttttcccgt
     3961 cgcaccgtgg gcaaccgcct ccgccccctc tttcatcgcg atttccgcct ggcggcgggc
     4021 gatcagcgga cgggcaatcg acgtgcccag cagatacgaa ccttcataca tcgcgttccc
     4081 gcgaagcatt ggaaacacgt actccctgac aaacgcttct cgcaggtcct caatatagat
     4141 tttctttaca ccgagtttct gggccttggc cttcacggcc tgcagatctt cgccttggcc
     4201 aagatcggca caaaacgcga tgacctctgc ctcatacgtt tcctgcaacc atttcaaaat
     4261 gacggaggta tccagcccgc cggaataggc cagtacgacc ttcttgattc cacggttcat
     4321 cgttatcctg gtctcctttt tcccagcagc ttcgtcagga tggctttctg catatgcagg
     4381 cgattttcag cctgatcgat aatcaccgac tgctgaccgt caagcatttc ggccgtgatc
     4441 tcttcaccgc ggtgagccgg cagacagtgc atgacgatcg cgtcgggctt ggctttggcg
     4501 acgagccggc tgttcacctg atacggcgca aggaccttca accgtctggc ctgttcccgc
     4561 tcccggccca tgctaatcca gacgtcggcg tagactacgt ccgcttcctt caccgcgacg
     4621 tacgggtctt gggcaacatc gatggtcgct ccggtcttga cggcttcaat tctggctaca
     4681 tcgaccacgt gttggtctgg ctgatagccg gatggacagc cgagcgcaat ggtcataccc
     4741 atcttggcca cggcctcgat gagagaattc gccacattat tgccgtctcc gacataggcg
     4801 atcttgatgc cccgtaacct tcccttcttc tctttgatcg tcagcagatc ggacaaggcc
     4861 tggcaggggt ggctcaagtc ggtgagcccg ttgatgacgg gcatggtcgc ctcgcgggcc
     4921 cactcctcta ccgtcgcatg gtcgtaggtc cggatgacga ttccatcgag atagcgggac
     4981 aacacacgcg cggtgtccgc cacactttca ccgcgcgata gctgaatgtc agccatcggc
     5041 agcactaacg catggccccc taattggttc attcctgctt caaacgagac acgcgttctc
     5101 gtggaaggct tttgaaacaa caagccgagc gttttgcctt gaagcagtcg gtgcgaggtc
     5161 cctcgctgtt gtttggcttt gagcacagtg gccaatgtca acagcccttc gatctcggcc
     5221 ctggacagtg cggcgacgtc caacaagtcc ttgaccggct tgggtcttcg ctcggacgct
     5281 cgtgtcatgg gctaaacggc cttagtgcga cgcggcctga gccgcgtgac ggtcgaagat
     5341 ctgcgagagc acgtcgagca gacgatcgac ttccggttga ctgataatca acggtgggac
     5401 gaaacggagc acgtggtctc cggtcgcgtt gatcaggatg ccccgtttca ggcagtcgct
     5461 gacgactgct ttggtgtcga tctcgatttc cataccttgc aggaggccaa gcccgcgtac
     5521 gtcgcgcacg agatgatggc aatccttgca ttccgacaag cctttactga ggtattcgcc
     5581 catgcggtga gcgtgatcga ggacgcgacc atcgaggagc acacggaaga ctgcgagcgc
     5641 tgcggcgcac gccagcgggt tgccgccgaa cgttgacgca tgcgagccgg ggccgaacgc
     5701 agccgctgca gacatcgtcg cgagacaggc cccgatcggg acgcctccac caaggccttt
     5761 ggcaagcgtc atgacatccg gttgaacccc gagctgctcg tacgcgaaca gggtcccagt
     5821 gcgccccatg ccggtttgca cttcgtcgaa gatcaggaga atatcttttt gcgtgcacag
     5881 gtcacgaacc gctttgagat agtcgcggcc ggcaacgtgg acacctcctt ctccttgaat
     5941 cggctccagc atgatggctg ctgtgttctc agtcacagcc tccttcaact cggccacgtg
     6001 attgaagggc acataggaaa agcccggaag aagcggttca taccccttct ggactttttc
     6061 ctgaccagtc gccgtcaatg tcgccatcgt gcggccatgg aacgattgct tcatcgtaat
     6121 aatttcaaaa cggccggcgc catacttgtc gtgtgaatat cgacgcgcca gcttgatcgc
     6181 ggcttcattg gcttctgccc cgctattgca gaaaaacacc ttatcggcga acgagtggtc
     6241 caccagcatt tgcgccagct tcacttgcgg ttctgtgtag tatagatttg aggtgtggag
     6301 aagttgcgcg gcttgttttt gaatggcatg aaccagatcg ggatgtccat accccagaat
     6361 attgacggcg attcctccga cgaaatcgac gtattcacgg ccctccaggt cgtacacctt
     6421 tgttccccgt cctcggacaa ttgagatcgg ttgacgggtg tacgtcccca ttagatactg
     6481 ttctgcgctt tccttgagct cttcggtcgg catgacagac gtatcccctt gtaaagtaag
     6541 ggaagctagc acaggaacga tgatgaaagc aaatcaacct tgaaacgact ggcccggcgc
     6601 ctttccagcc cgtgctcgca ggacgatgtg gtaacctctg agcgatgaaa ttctgtgcgc
     6661 agtgccagca gcagaatcaa gacgacgcga atttttgcca tcaatgcggc caggacctca
     6721 gcgctgagaa cgctggatct tccgtgtcct caacggttgc cccaacggcg tggagccgca
     6781 ccgatcatga acgctggcgt gactttatcg gtccgaatgc ggaccgttac ctcgaacagt
     6841 tcaagaaatt cggctcggcg caatcccccc ggttcgcctt aacctggaac tggccggcct
     6901 ttctgttcat ctcatttctg tggtttctct atcggaagat gtatctctat gcggccgtgt
     6961 acgcgatagg gcccatggtt tcaacctacc tgacggggga catgacgagc ggcatggtct
     7021 ggagcgtcgt agccggagcg actgcgaatt atgtctatta ttggcattgc cgtgagcaca
     7081 tcgcggcgat caaacaacaa gcctgggctg accattcgaa gcagcatctt gccatgaaag
     7141 aagccggcgg ggtgcaaccg tatgtcgtat gggtgggcgt cgtcttctat gcgctgttct
     7201 tcctcactat gtttaaaata gttcaggaag gagggctaga cgctgataaa gttcccacca
     7261 aagccaagcc cgccactatg acaagcgcct agcataccga caggggaacc agaatcgggc
     7321 caagtcaacg gcgtcgctgc cacgacatcc aggcctgaaa ccaactccag tcttgctcgt
     7381 atgcagcacc gcccgaatcg gccgactcct gcttctgttc cagttgggtg taggtgcggg
     7441 gccagttttt ctgccaccag ctcttgagtt catcgggacc atagccctgc ccgccctggc
     7501 ttagaatacc tgccgcagta cacagtggcg cgacaagcga ccagtagcgg tccttcccaa
     7561 gaccgagact acgaaaatgt tcccgcaaca agaacaccac ccagagttct gcactatgtt
     7621 tcttgttgtg cttaaagggt tgagtctgac gcaaggggac aggatcgaat gccttgacga
     7681 tcgaagtgta gtccggtcca ccataactac ccgccgcttc cgctatgcct tcgacgatgt
     7741 tcgataattc caactccgcc accggggaag cgctgatgtt ctgaccgggg gatgcctccg
     7801 aaatcgggct tggagtggtg agcaactcga ttaacggctt cagttcccgc agacgggcgg
     7861 cagcggcctt gagtatgtgt tccgattcca gccagtagtc gctatcgctt ttggtcaggc
     7921 gctcgcgacc ggccggcggc aacaccgtcg gcacccaata gcgcatgagg acaaagagaa
     7981 tgtaatccac ttgacctccg acatgcgcaa tgcggtcaag aagaccgtcg gcacccgctg
     8041 ccttgaaaaa cgattgcgcg cggtcagcca cctgcaactt ttcgcccagg cgtacccacc
     8101 aggcttcaac cgcgcgccat ggaccgttgg gcaacggaat cgtactcatc gaagggcaat
     8161 ccggtgaaat cgtgcggcat ggtactgccg caatagctcg aaagcaacgc ttgacttgaa
     8221 gtcctgttcc agtattcttt gtctcacacg ctgactcact tgcttggagg gaatggatgg
     8281 gccgactaat cctcttgcgt catggcgaat cgcagtggaa tctcgagaac cggtttacgg
     8341 ggtgggtcga cgtcccgctc tcagcacgag ggatacagga agcgaaaaac gccggcgaca
     8401 agctccgtcc cttccgcttc gaccgtgcct tcacgtccgt cttgcagcga gccaacgaaa
     8461 cgcttcgcct ggcactcgag gtgatcggcc aaacgacgat cccgacggaa aaggacaagg
     8521 ccttgaacga gcggatgtac ggcgaactgc aaggattgaa caaggcggaa actgcacaaa
     8581 aatacgggga ggaccaagtg aagatttggc ggcggagcta tgacgtccgg ccgccaggag
     8641 gagaaagtct caaagacacc gccgagcgtg tcttgcccta ttacgagaaa gccatcaagc
     8701 cgcatgtcct ggcgggcgag accattctca ttgccgcaca cggcaatagc ctgcgcgccc
     8761 tcgtcatgga gctggaacgg ctctcgaagg agcaggtcct ggaattgaac attccaactg
     8821 gcgcaccact gctctacgag ttgagcccga atggaaccgt cactaaccac cgctatttgt
     8881 aaccgcctcg catgcaagtc tccgcctgac ccagctcgcc gccccgcatc gacacccgat
     8941 cgtgcgagcg cgtacaatgc gtcctatgcc acaggcttct tcctcccatc cgccgctgtc
     9001 ccggttggat gatgccaccg aacagctcca aacacgcctt ctccgccagg tcggtcagac
     9061 gatcaacgac tatggattga tcgaagcagg cgataaggtg atggtctgtg tgtccggggg
     9121 aaaggacagt tacgggttac tggacctctt actgacgctt caacgtcggg cgcctattca
     9181 tttcgacttg atcgcggtca gcctggatca gaaacaaccc gggtatcccg cccatgtcct
     9241 gcccgagtat ctgaggtccg caggcgttcc cttccacatt gaagaacgtg atacgtattc
     9301 catcgtcaaa cggctcattc ccgagggaca aaccacctgc tcgttgtgtt cccggcttcg
     9361 ccgaggtcat ctctaccgca tcgccacgga acgaggggcc acgaaaatcg cgctgggaca
     9421 tcatcgtgat gacattctcg aaacgctgtt gttgaatctt ctattcaccg ggaagctcaa
     9481 agccatgccg ccgaagctcc gttccaaaga cggtcggcat ctggtcattc gtccgctcgc
     9541 cgccgtccgg gaagcggatc tgatccggta cgcggaactg cgcgcgttcc ccatcattcc
     9601 ctgcgatctg tgcgggtcac aagaaaacct caaacgcaaa caggcgaaag cattcttgct
     9661 ggaatgggaa cgtcttgccc cgggctgcac ggatagcatg ttcgccgcat tgtccaacat
     9721 cgctccgtcg ctgctgatgg atcaacggct gttcgatttt cgggtcttgc gccgcgacga
     9781 gtcggcggcc ggcgacgaag acgcttggtt ggatgagggc aacggctagg tcttaagctt
     9841 gctcgaaaag cgtgtgcgga acttcatcac ctttggcccg acaacggcgg tacagtagcc
     9901 ttgaaaggga ttccgagcga aatattcctg gtggtacggc tcggcttcat accatgccgg
     9961 agcagcgacc acttctgtca caatcgggct gggatagacc cgctcgttgg tgagtaatgc
    10021 gatcacgtct tccgcaattc gcttctgctc aggggtgtgg taaaagaccg ccgaccggta
    10081 ctgggtgccg atgtcatgcc cttgacggtt gagcgtcgtc ggatcatgaa tcacgaagaa
    10141 cgcctccagc aactctcgat aactgacgat ggccggatcg aacgaaatgc ggaccgcttc
    10201 cgcatgaccg gtacgtccgc cgcacacggc ctcataggta gggctctctt gatggccccc
    10261 tgtatagccg gactccacgg agttgacacc tcgcatctgg tcgtagaccg cttccagaca
    10321 ccagaagcat ccgcccgcca gtgtcgcaac ctcttccttc atgactccct gctgtgcaaa
    10381 ttgatcggtc gtgaacgata cgtcatggag acgcctcgtc cttatatcac atgaaacaaa
    10441 aggcctgcga gcagagacca cgggggggga attgagagcg gagcagtggt agtgctgtct
    10501 aaccggcttt cagatccgcg atgacttgct tcaacttggc ccgttcatcc ggccgcatag
    10561 ccacgaattg aagcccaaat gtcctgccct ggacccatcg cacttgcgcc tcctcaatgc
    10621 gcagtggcca gctgagaccg ggtgcgtaga tccggcattc gaatgaacca ttcggcacca
    10681 cggggcaatc ggtttctatt cggcagccgc catctgacag atccagtgtt ttcccatgac
    10741 cctcacgttg cccacccgaa aacgtgctgc gaaattgcgc ggtcagcctc ggatgcgccc
    10801 ttttttcctt cacgcgaggt ttcttgacta actgggatcg caacggcatc gatgtgtcct
    10861 ctcaaaatga cgaaccccga cggctctcac gacgagcatg cgacggggtg actcacgtcc
    10921 ttgagtcgtg actattttct tttcaacaga acgtgtccgc gacgattgag ctgatagcag
    10981 gattcatcat gttcgaggca aaatgggcgt tccttaccat aggacacgat gccgatctgt
    11041 ttgctactga gacctatttc agtgaggtaa ttgcgggcgc tcttcgatcg tttgtccccg
    11101 agtacgatgt tgtaatcagt cgtcccccgt tcatcgcagt ggccctcaac cttcaacacc
    11161 gcccccgggt gctctttcaa ccacttggcg ttgtgattga gtgcctccat cccggaatcc
    11221 gacagcgtcc actgatcaaa cccaaagaag acatccttca gaccggcctc cacggcggct
    11281 tgctcctctt tcgacaattc cgcacgatgg cgcataccac ccgtggccgg tgagacggag
    11341 gcgatgccgc caccgtgaga aaggtattca tccgatggat tctgggaaaa tccgctcaac
    11401 tcgttgcgaa ggccgccttg actcataccc gggaaatcgg gattgacgcc ttggccatgc
    11461 catcctgagc cattggttga accggccatt tcaccacctt ctgactgtaa ccatttcatc
    11521 ccgcatccag ccgtaccgag cacggtcaga cccaaagctg ctactagaac gctcttactt
    11581 agactggcct tcatggtgtt tcccccatcc ctgttgtggc agtttactta ctggaacaac
    11641 tatagcaccg gatttacaag ggaggtttca acaggaagag acgtgcgaag gcatgaatta
    11701 tatgaggttt tttgacaggg gccgtagacg aggcctacag ggcagaactg gtgctttaca
    11761 caaccgctag aactgcgccg aacgccgatc cgcccaacgc acgggacgca tgaccgtacc
    11821 caaggctgcg accgcgcaga tcatggccag catgagcaaa cccatcatca agaagaatgc
    11881 ggcccttacg tcatggagga taacatgatt catcaaattt tccctccttg ttgaatcggt
    11941 tcttacccgc tttgccagcc acaacggcag agccaccggg ccatgggatc aattttggtc
    12001 aaccagcatc cgcaacaggg gcagtgcctt gtccatactt gccttctgcc acgatcaagg
    12061 catccggttg gtttcccatc gggatgtctc atcagacctt gtccatagaa taccactttc
    12121 gaactcctct ggtcttctcc aaccttgttt tagcctacaa cgggaaaggt caaaaatcca
    12181 tgcagcttaa gtggggaggg ggcggagcca gcccgcgatt actgcggcta agtacttgaa
    12241 gtga
//
LOCUS       DASTQY010000188        14174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_148228, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000188 DASTQY010000000
VERSION     DASTQY010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 14174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14174
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148228"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /gene="tuf"
                     /locus_tag="VFR82_06225"
     CDS             complement(<1..1175)
                     /gene="tuf"
                     /locus_tag="VFR82_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU4501232.1"
                     /translation="MAKAKYERKKPHVNIGTIGHVDHGKTTLTAALTKISADKGMAKF
                     ISYDEVAKASESQGRRDATKIMTIAISHVEYETDKRHYAHVDCPGHADYVKNMITGAA
                     QMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADKIDDKELLDLVELEVR
                     ELLAKYGFPGDKTPIIQGSALKAIEGDQGPLGVPSILKLLEAVDSYIPTPQRPIDKPF
                     LMPIEDVFTISGRGTVVTGRCERGIVKVGDEIEIVGLRPNQATVVTGVEMFRKVLDEG
                     QAGDNVGVLLRGTKKEDVERGMVLAKPKSITPHTKFKAEIYVLTKEEGGRHTPFFNGY
                     RPQFYFRTTDVTGVVSLNPGVEMVMPGDNVSVTAELISPIAMDQGLRFAVREGGKTVG
                     "
     gene            complement(1219..3300)
                     /gene="fusA"
                     /locus_tag="VFR82_06230"
     CDS             complement(1219..3300)
                     /gene="fusA"
                     /locus_tag="VFR82_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU4501233.1"
                     /translation="MARQAPLERTRNIGIMAHIDAGKTTTTERILYYTGMTHKMGEVH
                     EGAATMDWMEQERERGITITAAATTCFWKDHRINIIDTPGHVDFTIEVERSLRVLDGA
                     VAVFDSVQGVEPQSETVWRQADKYQVPRIAFMNKMDRIGADFYFSVQTIIDRLGANPV
                     PIQIPIGRETEYRGSIDLITMKAYVYDDETLGAKYKVEEIPADLAEKAKEYREKMLES
                     VAEFDDQVMEKYLNGQSLTEEEVRRVVRAGAIALKITPVLCGSAFKNKGVQQLLDGVV
                     DFLPSPLDIPAVKGIDPNTNKELERKPADTEPFSALAFKIMSDPFAGQLTYFRVYSGT
                     LKTGTPVLNVTKGTKDRIGRLLKMHANKREEIDAAHAGDIVAAVGLKGATTGDTLADE
                     KQPVLLEVMKFPEPVIAMAIEPKTKQDQEKMGFALQKLAQEDPSFRVRTDEETAQTII
                     AGMGELHLEIIVDRLLREFKVEANVGKPEVAFRETIRRKAEAESKYIKQTGGRGQYGH
                     VVMTVEPSESGKGLEFVNKVVGGAIPKEYIPAIEKGVKERMENGVIAGYPLRDIKVTV
                     IDGSYHEVDSNEMAFKIAASMGFSDACKKADAVLLEPIMKVEVLVPQEFMGDVIGNLN
                     GRRGKVQGMKVRAGAQAIEASVPLMEMFGYATDLRSRTQGRATYSMEFDRYEQVPKQI
                     SEAIIAKYRGE"
     gene            complement(3412..3882)
                     /gene="rpsG"
                     /locus_tag="VFR82_06235"
     CDS             complement(3412..3882)
                     /gene="rpsG"
                     /locus_tag="VFR82_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU4501234.1"
                     /translation="MPRSRFLGQREPLPDVRYRDKIVGKFLNILMEGGKKSTAERICY
                     GAFDVIQEKTGGDPLKVFRSAIDNVKPIVEVKSRRVGGASYQVPVEIRPARRMSLALR
                     WLSEYARTRGGKSMREKLAAELLDASNNTGAAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3919..4290)
                     /gene="rpsL"
                     /locus_tag="VFR82_06240"
     CDS             complement(3919..4290)
                     /gene="rpsL"
                     /locus_tag="VFR82_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU4501235.1"
                     /translation="MPTINQLVRKGRQLAHAKTKSPALKSCPQKRGVCLRVYTSTPKK
                     PNSALRKVARVRLTNGMEVTTYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGAL
                     DAVGVADRKQGRSKYGAKRPK"
     gene            complement(4405..8529)
                     /gene="rpoC"
                     /locus_tag="VFR82_06245"
     CDS             complement(4405..8529)
                     /gene="rpoC"
                     /locus_tag="VFR82_06245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU4501236.1"
                     /translation="MRIRIASPEKIRSWSYGEVKKPETINYRSFKPEKDGLFCAKIFG
                     PIKDWECNCGKYKRMKHRGIVCDKCGVEVIQSKVRRERMGHIELAAPVAHIWFLKGVP
                     SRIGTLLDMSLKQLEKILYFESYVCVDAGQTDLTEKDLVTEEKLRQLQSEYAPNAYKV
                     GIGADAIRELLRKIDINSKWDEIKAKAKVSTSAAMKKKFAKQLKVIEAFRKSGNKPEW
                     MIMDVIPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLMELKAPGVIIRN
                     EMRMLQEAVDALFDNGRRGRAIRGPNKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGR
                     TVIVVGPELRLHQCGLPKKMALELFKPFIFHKLEERGAATTIKSAKRLVEKERPEVWD
                     VLDEVIREHPVLLNRAPTLHRLGIQAFDPVLVEGKAIRLHPLVCAAFNADFDGDQMAV
                     HVPLSVEAQVEARVLMMSINNILSPANGKPIAVPSQDMVLGCYWLTKERVGVKGEGKV
                     FGSTEEVRIAFDAREVEEHARIKVRIEGNLVQTTVGRVILSEVLPAGMPFAYANKLMT
                     KKEVTKLIDSVYRQSGHRETVMFLDKIKDAGFTYATRAGVSICIDNMHIPSKKEELIG
                     KAQREVNEIERQYAEGLITNGERYNKVIDIWAHVTEQVAGEMMKELGAGGDPNKDEAF
                     NPIFMMADSGARGSSQQIRQLGGMRGLMAKPSGEIIETPITANFREGLTVLQYFISTH
                     GARKGLADTALKTANSGYLTRRLVDIAQDVIISEIDCGTTDGIFVSALVEGGEIIQPI
                     EERILGRLAAEDIRDPVTGEVIVSFNEEITEERTKDVVEAAVDRVKIRSVLTCQSRRG
                     VCRACYGRDLARGRLVEKGEPVGVIAAQSIGEPGTQLTMRTFHIGGTASKVVEQTVLE
                     AKHAGRMKFMSFDAKKNADVHNAGIAVRNKENEWVVMNRNAKIAIVDENGREREKYPV
                     VYGAKIKIKDGDRVELAQKLVEWDPYSLTILTEVGGKVAYGDIVEGVTMKEEFDEVTG
                     LSRKVIIEHSGATLRPRVSIKDEGGKTAKVSGGSNTVARYLLPVGAHIFVEKGAAVHP
                     GDVLAKIPRETTKTKDITGGLPRVAELFEARKPKEQAVISEIDGEVSYGGFVKGQRKV
                     LVDNKMGDVKEYFIPKGKHVNVHEGDWVRAGEPLMDGSANPHDILDVLGPNELQKYLV
                     DEVQDVYRLQGVSINDKHIEIIVRQMLRKVRIEDPGDTEFLPGSQVSKMLFEEENDRV
                     LKKDGKPALGKPVLLGITKAALTTDSFISAASFQETTRVLTEAAINGREDNLLGLKEN
                     VIVGRLIPAGSGFEEYRDTFVISPKPEPVVVGSTEPAALPREGAAAATGEGAGG"
     gene            complement(8629..12585)
                     /gene="rpoB"
                     /locus_tag="VFR82_06250"
     CDS             complement(8629..12585)
                     /gene="rpoB"
                     /locus_tag="VFR82_06250"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU4501237.1"
                     /translation="MPETTLQEFVERKDFSRIRSSIDIPDLIEIQKRSYEEFLQFEVE
                     PERRKDHGLQAALASVFPIPDYNNTAILEFTSYTLGTPKYDERECLEQGMTFAVPLKL
                     RVRLVVFDKEDKGPKKKVLDVREQEVYVGELPLMTERGTFIVNGTERVVVSQLHRSPG
                     ASFTHDKGRTHASGKVLYSARIIPYRGSWLDFEFDARDILYVRIDRRRKMPATILLKA
                     FGFSSDDLLKMYYPVEEIRVNKGKLMRKLDPEIHQGMRCSTEVVDKSGKEPLVREGGK
                     LTKGIIAKLKAAGVKEIPVAPSELVGRAVLSEVVDTKKNKLVEKNQRLTAEIIEKILE
                     SEIEEFKVIFFDAATSTPVILDTLEMERTVSKEEAMVEIYRRLRPGETPSVETARALF
                     DNLFLNSKRYDLSPVGRLKLNKKLLLDLPLEQRTLTSQDIVEVIRYLVNLKMGKGEID
                     DIDHLGNRRVRSVGELLENQFRLGLVRMERSIKERMNLLDMETVLPHDLINAKPVVAA
                     VKEFFSSSQLSQFMDQTNPLAEITHKRRLSALGPGGLTRERAGFEVRDVHPSHYSRIC
                     PIETPEGPNIGLITSLATYARINEFGFIEAPYRRVDKGRVTDQIEFLSAIEGDKFLIA
                     QANSKVDGSGRLVSETVSARSGGDFVTITPDRVEYMDVSPKQVVSVATALVPFLEHDD
                     ANRALMGSNMQRQAVPLLKSEAPLVGTGMEAVVARDSGYVVQARRSGVVESVDATRIV
                     VRSDSKEGRKRNDTGLDVYDLIKFQRSNQNTCITQTPVVRIGQPVKKGQVLADGPAID
                     QGELALGKNVLVAFMPWGGYNFEDAILLSEKLVREDAFTSIHIEEFEVEARDTKLGKE
                     DITRDIPNVGEEALRNLDESGIIRIGAEVKPGDILVGKVTPKGETQLTPEEKLLRAIF
                     GEKAGDVKDTSLTVPPGVEGIVVDVKIFSRKGLDKDERSKSIESEDTMRLQRDHHEEL
                     RIIDEEKTKKVRKLLLGKVVGRDLMDPESGDVILKKKGKLTAEILKRLSDESVRYIIL
                     SDPDEQKELEDVERRAKEQVEILQTLYDEKVGRLKRGDELPPGVIKLVKVYISMKRKI
                     QVGDKMAGRHGNKGVVSRVLPEEDMPYLPDGTPVEIVLNPLGVPSRMNVGQILETHLG
                     WAAKALGIKVASPVFDGASEKEIKDLLKKGKLPTSGQSLLMDGRTGEAFGSPVTVGYM
                     YVLKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWALQAYGAASTLQ
                     EFLTVKSDDVPGRSRMYEAIVKGEPFLEPGLPESFNVLVKELQSLGLDVELVKTQD"
     gene            complement(12662..13063)
                     /gene="rplL"
                     /locus_tag="VFR82_06255"
     CDS             complement(12662..13063)
                     /gene="rplL"
                     /locus_tag="VFR82_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU4501238.1"
                     /translation="MSATATKVSQEELIKAIESMSVLDLAELVKGLESRFGVTAAAPV
                     AVAAAPAAGGAAAAPAEEKTAFDVILASAPADKKIQVIKVVRELTNLGLKEAKDLVEG
                     APKPVKTGATKEESDAMKKKLEESGAKVEIK"
     gene            complement(13091..13597)
                     /gene="rplJ"
                     /locus_tag="VFR82_06260"
                     /pseudo
     CDS             complement(13091..13597)
                     /gene="rplJ"
                     /locus_tag="VFR82_06260"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
     gene            complement(13661..>14174)
                     /gene="rplA"
                     /locus_tag="VFR82_06265"
     CDS             complement(13661..>14174)
                     /gene="rplA"
                     /locus_tag="VFR82_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU4501239.1"
                     /translation="LPHGTGKKLRVLVFAKGEKEQEARQAGADFVGSDDLMEKIKGGW
                     MDFDCAIATPDLMASVGKLGKQLGPRGLMPNPKTGTVTFEVGKAVGEIRKGRVEYKVE
                     KAGIVQVPVGKVSFQPDQLYDNASAVLEAVVKAKPASCKGRYLKSVTISSTMGIGVKL
                     DAIALAKQWS"
BASE COUNT         2674 a         4458 c         3618 g         3424 t
ORIGIN      
        1 ccgaccgtct tgccgccctc acgcacggca aagcgcaggc cctggtccat cgcgatcggg
       61 ctgatcaatt cagccgtcac gctcacgttg tcgcccggca tcaccatctc cactcccgga
      121 ttcaacgaca ccacgcccgt cacgtcggtg gtccggaaat agaactgcgg ccgatacccg
      181 ttgaaaaacg gcgtatggcg gcccccttct tccttcgtca gcacgtagat ctccgccttg
      241 aacttcgtgt ggggggtaat gctcttgggt ttcgccagca ccatgccccg ctccacatct
      301 tcctttttgg ttccccgcaa cagcacgccc acattgtcgc cggcctgccc ttcatcgagc
      361 accttgcgaa acatttcaac gccggtgacg acggtggcct gattcggccg cagccccacg
      421 atctcgattt catcgccgac tttcacgatg cctcgctcac agcgccccgt caccaccgtg
      481 ccccggccgc tgatcgtgaa gacgtcttcg atcggcatca aaaacggctt atcaatgggg
      541 cgttgaggag tcgggatgta gctatccacg gcctccaaca acttgagaat cgagggcact
      601 cccaaggggc cctgatcgcc ttcgatggct tttaacgcac tgccctggat gatcggcgtc
      661 ttgtcccccg ggaagccata cttggccaac agctcccgca cttccagctc caccaggtcc
      721 aacagttcct tgtcgtcgat cttgtcggcc ttgtttaaaa acaccacgat gtagggcacc
      781 cccacctggc gggccaacag gatgtgctcc cgcgtctggg gcatcgggcc atcggcggca
      841 ctcaccacca aaatcgcccc gtccatctgc gccgcccccg tgatcatgtt cttcacgtag
      901 tccgcgtgcc ccgggcagtc cacgtgcgca tagtggcgct tatcggtttc gtattccacg
      961 tggctaatcg caatcgtcat gatcttggtg gcatcgcggc gcccctgact ctcgctcgcc
     1021 ttcgccactt cgtcgtagct gataaacttc gccatcccct tatccgcgct gatcttcgtc
     1081 aaggccgccg tcaacgtggt cttcccatgg tccacgtgcc cgatcgtccc gatattcaca
     1141 tgcggcttct tgcgctcata tttcgccttc gccatacctc aactccttct cagcaagtgt
     1201 cgtcagtcgg atcagccgtt actcgccacg gtacttggca ataatggctt ctgaaatctg
     1261 tttaggcacc tgttcgtatc gatcgaattc catactgtat gtggcgcgac cctgcgtgcg
     1321 ggaacgaagg tccgtcgcat agccgaacat ttccatgagc ggcaccgacg cttcgatcgc
     1381 ctgagcaccg gcacgcacct tcatgccttg aaccttgccg cgtctgccgt tgaggttacc
     1441 gatgacatcg cccatgaatt cctgaggtac gagcacctcg actttcatga tcggctcgag
     1501 aagcactgca tcagccttct tgcaggcatc ggaaaatccc atcgacgcag cgatcttgaa
     1561 ggccatttcg ttcgagtcga cctcgtggta cgacccgtca atgacggtca ccttgatatc
     1621 gcgcaacgga tagccggcaa tcacgccgtt ctccatacgc tctttcacgc ctttttcgat
     1681 cgccgggatg tattccttgg ggatagcccc tcctacgacc ttgttgacaa attccaaccc
     1741 cttaccagac tccgatggct cgaccgtcat caccacgtga ccatactggc cgcgaccgcc
     1801 ggtctgcttg atgtacttcg attctgcttc ggcttttcgc cggatggttt cgcggaaagc
     1861 aacttcgggc ttgcccacgt tcgcttcgac tttgaattct cgcagcagcc ggtccacgat
     1921 gatttccagg tgcaattcac ccatccccgc aatgatggtt tgcgccgttt cttcatcagt
     1981 gcgcacccgg aaggacgggt cttcctgggc cagcttttgc agagcaaagc ccatcttttc
     2041 ttgatcctgc ttggtcttcg gttcaatggc catggcaatg accggctccg gaaacttcat
     2101 cacttcgagc aggacaggct gcttttcatc ggccaacgtg tcaccggtgg tggcgccctt
     2161 cagcccgacc gcagccacaa tgtcacccgc atgggcagca tcaatttctt cacgtttgtt
     2221 cgcatgcatt ttcagcaggc gaccaatgcg atccttcgtg ccctttgtca cgttcaggac
     2281 cggcgttccg gttttcaacg tcccggagta cacccggaaa taggtgagct ggccagcaaa
     2341 tggatcggac atgattttga atgccaaggc ggaaaacggc tcggtatctg caggcttccg
     2401 ttccagttct ttgttcgtat tgggatcgat gcctttaacg gcggggatat ccagagggga
     2461 gggcagaaaa tccacaaccc cgtcaagcag ctgctgcacg cccttgttct taaaggccga
     2521 tccgcagagc acgggagtga tcttcagcgc gatcgcgccc gcccgtacca cccgccgaac
     2581 ctcctcttcg gtcaacgatt gaccgttcaa gtacttttcc atgacctggt cgtcgaattc
     2641 ggccaccgat tcgagcattt tctcgcggta ttctttggct ttctcggcga gatcggccgg
     2701 gatctcctcg accttgtatt tcgctccgag ggtttcgtcg tcgtacacgt acgccttcat
     2761 cgtgatcaga tcgatagagc cccggtattc cgtttcccgg ccgatcggaa tctgaatcgg
     2821 cacggggttc gcgcccaaac ggtcgatgat ggtctgcacg ctgaagtaaa aatctgcccc
     2881 gatacgatcc atcttgttca tgaatgcgat acgaggcacc tggtacttgt ccgcctgccg
     2941 ccagaccgtt tcggactgcg gctcgacgcc ttggaccgag tcgaacacgg cgacggcccc
     3001 gtccaggacg cgcaacgacc gttcgacctc gatcgtaaag tcgacgtgac ccggtgtatc
     3061 aatgatattg atgcggtggt ctttccaaaa acaggtcgtc gcggcggcgg taatcgtgat
     3121 cccgcgctcc cgttcctgtt ccatccagtc catcgtggct gcaccctcat ggacctcgcc
     3181 catcttgtgc gtcatcccgg tgtaatagag gatgcgctcc gtcgtcgtcg tttttccggc
     3241 gtcgatgtgc gccatgatgc cgatgtttct ggttcgctcc agtggtgctt gccttgccat
     3301 gttatccctt taaaaacaca cgtgctgcag gatgagccga aacaccgctg cgattcaggg
     3361 gcacccgcgt ctgcggatcg cagcacggct caactgcagc acgaaacgac gttaccagcg
     3421 atagtgcgcg aaggccttgt tcgcctccgc catccgatgc acgtcttccc gtttcttgac
     3481 cgcggcccct gtattgttcg acgcgtccaa cagctcggcc gcgagctttt cccgcatgct
     3541 cttgccgccc cgcgtcctcg catactctga caaccaccga agggcgagag acatccggcg
     3601 cgccggacga atttcgaccg gcacttggta cgaagcaccg ccgactcggc gggatttcac
     3661 ttcgacgatc ggtttcacgt tgtcgatagc cgaccgaaat actttcagtg gatctccccc
     3721 ggttttttcc tgaatgacgt cgaaggcacc gtaacaaatc cgctcagccg tactcttctt
     3781 gccgccttcc atgaggatgt tgagaaactt ccccacaatt ttatcgcggt atcgaacatc
     3841 tggcagcggc tctctctgtc ctaaaaatct gcttcgtggc atagttgatt actttcgtga
     3901 gcctagtaaa gtggggcctt atttcggacg cttggcgcca tatttggagc ggccctgctt
     3961 ccgatcagca accccgacgg catcgagcgc gcctcgcaca atgtgatagc gcacgcccgg
     4021 caagtccttg acgcggccac cgcgaaccag cacgattgaa tgttcctgta agttatgccc
     4081 gacgccggga atgtaggtcg taacctccat tccgttggtc aatcgtacgc gtgccacttt
     4141 gcgaagcgcg gaattcggct tcttcggtgt cgatgtgtat acgcgcaggc agacgcctcg
     4201 tttttgcgga caagacttga gcgccggact tttggttttg gcgtgggcca attgacggcc
     4261 ctttctcact aactgattga tcgttggcat cgttcctgtc tttcagcttc aacgcgatag
     4321 gttcctgaca gccccttcaa gggcaaagcc cgcggattat agtgatgccc gcgggcgctg
     4381 tcaagcgacg aaacccctga ccggttaccc gcctgcacct tcgccggtcg ctgcagccgc
     4441 cccttcacgc gggagcgcag caggctcagt cgaaccgact acaaccggct caggcttcgg
     4501 gctgatgacg aaggtgtcgc gatattcctc aaacccgctg cccgcaggga tgaggcgacc
     4561 gacgatcacg ttctccttca atccgagaag attgtcctct ctgccattga ttgccgcctc
     4621 ggtcagcaca cgggtagtct cctggaatga cgccgcagag atgaaactgt cggtcgtcag
     4681 tgccgccttc gtgatgccaa ggagaacggg tttgccgagc gccggctttc cgtccttctt
     4741 caacacgcga tcattttctt cttcaaatag catcttgctg acctgactgc ccggcaagaa
     4801 ctccgtgtcg ccgggatctt caatccgcac cttgcgcagc atttgacgca cgatgatctc
     4861 aatgtgcttg tcgttgatcg acacaccctg gaggcggtag acgtcctgca cttcgtcgac
     4921 caggtacttt tgcagttcgt tgggtccgag cacgtcgaga atgtcgtgcg gattcgcgga
     4981 gccgtccatc aacggctctc cggcccgcac ccaatcgccc tcgtgcacgt tgacgtgctt
     5041 ccccttggga atgaaatact ctttgacgtc gcccattttg ttgtcgacca ggaccttgcg
     5101 ttgacctttg acgaatccac cgtacgacac ttcgccgtcg atttcgctga tgacggcctg
     5161 ttctttcggc tttctcgcct cgaataactc ggccacacgc ggcaaaccgc cggtgatgtc
     5221 cttcgtcttg gtcgtttccc tgggaatctt cgccagcaca tcccccggat ggacggccgc
     5281 gcccttctcg acaaagatgt gtgcgccaac gggcaacaga tagcgggcca cggtgttgct
     5341 gccgcccgat acctttgccg tttttccgcc ttcgtccttg atcgacacgc gaggacgcag
     5401 cgtcgcgcct gaatgctcga tgatgacctt gcgtgagaga cccgtcacct catcgaactc
     5461 ctccttcatg gtgacgccct cgacgatgtc gccgtaagcc actttcccgc cgacttcggt
     5521 caaaatcgtc agtgagtacg ggtcccattc gacgagcttc tgcgcgagtt caacgcggtc
     5581 tccatccttg atcttgatct tggcgccgta gacaaccggg tacttctcgc gctcgcggcc
     5641 gttttcgtcc acgatggcga ttttcgcgtt gcggttcatc acgacccatt cattttcctt
     5701 gttacgcacg gcaatgccgg cgttgtgcac gtccgcattc ttcttcgcgt caaagctcat
     5761 gaacttcatg cgtccggcat gtttggcttc caagacggtt tgttccacga ccttgctcgc
     5821 cgtaccaccg atatggaatg ttcgcatcgt cagctgggta cccggctcgc cgatagattg
     5881 cgcagcaatg acaccgaccg gctcaccttt ctcgacgaga cgcccgcgcg ccagatcgcg
     5941 cccatagcaa gcccggcaga cgccacgacg ggactggcac gtcagcacgg accggatctt
     6001 gacccgatcc accgcggctt cgaccacatc cttcgtgcgc tcttcggtga tctcctcatt
     6061 gaaggagaca atcacctcac ccgtgaccgg atcacggatg tcctcagcgg ccaaacgtcc
     6121 caggatacgc tcttcgatag gctggatgat ttccccacct tcgaccaatg cgctgacaaa
     6181 aatgccgtcc gtcgtcccgc aatcgatttc actgatgatg acatcctgag caatgtcgac
     6241 cagccggcga gtgagatagc ccgagttcgc cgtcttcagt gcggtgtcgg ccaacccttt
     6301 gcgggcgccg tgcgtagaga tgaagtattg caacacggtc aagccttcac ggaaattcgc
     6361 cgtaatcggc gtctcaatga tttcgcctga cggcttagcc atcagtccgc gcataccgcc
     6421 caactggcga atctgctggg aactgcctcg cgctcccgag tcggccatca tgaagatggg
     6481 gttgaaggct tcatccttgt tcggatctcc gccagcaccc aactccttca tcatctcacc
     6541 cgccacctgc tcggtgacgt gcgcccagat gtcgatgacc ttgttgtagc gttctccgtt
     6601 ggtaatcaat ccttcggcat actgccgttc aatctcgttg acttcccgct gcgccttgcc
     6661 gatcagttct tccttcttac tggggatatg catattgtcg atgcagatcg aaacgccggc
     6721 gcgagtcgca taggtgaagc cggcgtcctt gattttgtcc aagaacatga cggtttcccg
     6781 atgccctgac tgacgataga cagagtcgat gagcttggtc acttccttct tcgtcattaa
     6841 tttattggca tacgcgaacg gcatccccgc aggcaacacc tcggagagta tgacgcggcc
     6901 gaccgttgtt tgaaccaggt tcccctcgat gcgaaccttg atgcgggcgt gctcctccac
     6961 ttctcgcgcg tcgaatgcaa tccgcacttc ctcggtggag ccgaagacct tgccctcgcc
     7021 cttgacccct acccgctctt tggtcaacca gtagcacccc agcaccatat cctgcgacgg
     7081 cacggcaata ggcttgccgt ttgccggcga gagaatgttg ttgatcgaca tcatcaatac
     7141 ccgcgcttca acctgcgctt ccacggacaa cggcacatgc acggccatct gatcgccgtc
     7201 gaagtcggca ttgaacgccg cacagaccag gggatgcaac cgaatggctt tgccttctac
     7261 cagcacggga tcgaaggctt ggatgcccaa tcgatggagc gtcggcgcac gattgagcaa
     7321 cacggggtgc tcacgaatca cttcgtcgag cacatcccag acttccggtc gctctttctc
     7381 aaccaaacgc ttggcgctct tgatcgtcgt cgcagcaccg cgctcttcca atttgtggaa
     7441 gatgaacggc ttgaacagtt ccagcgccat cttcttcgga agaccgcatt gatgcagccg
     7501 tagctctgga ccgaccacga tgaccgtgcg gccggaatag tccacgcgtt taccgagaag
     7561 attttgacgg aaccgccctt gctttccttt gagcatgtcg ctcaaggact tgagcggacg
     7621 cttgttgggt ccacgaatcg cccggccacg ccgaccgttg tcgaacaacg catcgaccgc
     7681 ttcctgtaac atgcgcattt catttcggat aatgacgccc ggtgccttca actccatcag
     7741 acgcttcaac cggttgttcc ggttgatgac gcgccgatac agatcgttca agtccgatgt
     7801 cgcaaagcgg ccgccgtcca gcgggacgag cggacgtaat tccggcggca gaaccgggat
     7861 cacatccatg atcatccact cgggcttgtt gccggacttg cggaaggctt cgatgacttt
     7921 cagctgtttc gcaaattttt tcttcatcgc cgcggaagtg gacaccttgg ctttcgcctt
     7981 gatctcatcc cacttcgagt tgatgtcgat cttccgcagc agctcgcgga tcgcatcagc
     8041 accgatgccg actttatagg cattgggggc atactcggac tgcagctggc gcagcttctc
     8101 ctcggtcacc aggtccttct ccgtcaaatc cgtctgcccg gcatcaacgc atacgtagct
     8161 ttcgaaatag agaattttct cgagctgctt caggctcatg tcgagtaacg ttccgatgcg
     8221 gctcggtacc ccttttaaaa accagatatg ggccaccggc gcggccagtt cgatatgacc
     8281 catccgctcg cggcggactt ttgactgaat gacttccacc ccgcacttgt cgcagacgat
     8341 gccgcgatgt ttcatccgct tatacttacc gcaattgcat tcccagtcct taatgggacc
     8401 gaagatcttg gcgcagaaca aaccgtcctt ttccggctta aacgatcgat aattgatcgt
     8461 ttccggcttc ttcacttcgc cgtaagacca ggatcggatt ttttccggcg atgcaatgcg
     8521 aatgcgcatt gaatcgaacg agaccgaatc ccgcggcttt tcaaataatg tgtatacgcc
     8581 ttccaaagta gtgacctcct ctgataccgg cagtgccggc tcaagtggtt agtcttgcgt
     8641 cttcaccaac tcgacgtcca atcccaaact ctgcagctcc ttgaccaaga cgttgaacga
     8701 ctccggcaat cccggctcga ggaacggctc gcctttcacg atcgcttcat acattcgcga
     8761 tcgcccgggc acgtcgtcgg atttcaccgt caaaaattcc tgcagggtgg aggcggctcc
     8821 atatgcctgc aacgcccaca cttccatttc gcccaaccgc tggccgccga actgagcctt
     8881 gccgcccaac ggctgctgcg taacgagcga ataaggcccg atcgatcgcg cgtgaatctt
     8941 gtcgtcgacc agatggtgga gcttcaacac atacatgtag cccaccgtca ccgggctgcc
     9001 gaaggcttca cccgtccgcc catccatgag cagcgattgt ccgctggtcg gtagtttgcc
     9061 ctttttcagc agatccttga tttccttttc agaggcccca tcgaacaccg gactcgccac
     9121 tttaatgcca agcgctttcg ccgcccaacc caagtgcgtc tccaagatct gtccgacgtt
     9181 catgcgtgac ggaacgccca gcggattcaa tacgatctcc accggcgtcc catcgggcaa
     9241 atatggcatg tcctcttcgg gcaacacgcg tgacacgacg cctttgttgc cgtggcggcc
     9301 ggccattttg tcgccgacct gaattttccg cttcatcgag atgtagacct tcacgagctt
     9361 gatgacgccg ggcggtaatt cgtcgccccg tttcaatcgc ccgacttttt cgtcgtaaag
     9421 cgtctggaga atttcgacct gttctttggc cctgcgctcg acatcctcca gttccttttg
     9481 ttcgtccgga tcgctgagaa tgatgtagcg aacggactcg tcggagaggc gcttcaagat
     9541 ctcagcggtg agcttgccct tcttcttcag aatcacatca ccgctctcag gatccatcag
     9601 atctcgcccg acgaccttgc cgagcaacag ctttcggact ttcttggtct tctcttcatc
     9661 gataatccga agctcctcgt gatggtcgcg ctgaagccgc atggtatctt cgctctcgat
     9721 gctcttcgac cgttcgtcct tatcaaggcc ctttcgggaa aagatcttca cgtcaacgac
     9781 gataccttct acccctggcg ggaccgtcaa ggatgtgtcc ttaacgtcac cggctttctc
     9841 accgaaaatg gcgcgcaaga gtttttcttc cggcgtgagc tgcgtttcgc ccttgggcgt
     9901 gacctttccg accaggatgt cgcctggttt cacttcggcg ccgatgcgaa tgatgccgct
     9961 ctcgtcgaga ttgcgcagtg cctcttcgcc gacgttcgga atatcacgcg taatatcttc
    10021 ctttccaagc ttggtatcgc gggcttcgac ttcgaactct tcaatatgga tggatgtgaa
    10081 cgcatcctct cgaacgagtt tctcactgag gagaatggcg tcttcgaagt tgtacccgcc
    10141 ccacggcatg aacgcgacga ggacgttctt ccccaatgct aattcgcctt gatcaatcgc
    10201 cggcccgtcg gcaagaacct gccccttctt gaccggttga ccgatccgca cgaccggcgt
    10261 ctgtgtgatg caggtattct ggttggatcg ctgaaacttg atcagatcgt acacatccaa
    10321 acccgtatcg ttccgtttcc gcccctcctt gctgtcggag cgcacaacga tccgcgtcgc
    10381 atcaacgctt tcgacgacgc ccgatcgcct ggcctggacg acgtagcccg agtcgcgcgc
    10441 cacgaccgct tccatgccgg tgccgactaa cggcgcttcc gatttgagta gcggtaccgc
    10501 ttgccgttgc atgttggagc ccatcagtgc gcggttcgcg tcatcgtgct cgaggaacgg
    10561 cacgagggcc gtcgccacgc tgaccacctg cttcggtgac acgtccatat attccacacg
    10621 gtccggggtg atcgtcacga aatcgccgcc ggatctcgct gaaaccgtct ctgataccaa
    10681 ccggccggag ccgtccacct tggagttggc ctgagcgatc aggaacttgt ctccttcgat
    10741 cgcggagaga aattcgatct gatcggtgac acggcccttg tccacgcgcc tgtagggcgc
    10801 ttcgatgaag ccgaactcgt tgatcctggc gtaggtcgcc agcgaggtga tcaagccgat
    10861 attcggacct tccggcgttt cgatcgggca gatccggctg tagtgagatg ggtgcacgtc
    10921 tcgcacttcg aatcccgcgc gttctctggt caagccgcct ggacccagtg ccgagaggcg
    10981 ccgtttgtgt gtgatttcag ccaacggatt cgtttggtcc atgaactgcg ataattggct
    11041 gctgctgaaa aactctttga ccgcagccac caccggcttg gcattgatca agtcgtgcgg
    11101 cagcaccgtc tccatatcga ggaggttcat gcgctctttg atgctgcgct ccatcctgac
    11161 aagacccaga cggaactggt tttccaacaa ctcgccgacc gaccggaccc gtcgatttcc
    11221 caagtggtcg atgtcgtcga tttctccttt tcccattttc aggttcacga ggtagcggat
    11281 aacttcgacg atatcctgcg aagtaagcgt ccgttgttcg agcggcaggt cgagcagcaa
    11341 cttcttgttg agtttcaatc gtccgaccgg agacaggtcg taccgcttgg agttcaaaaa
    11401 caaattgtcg aacagcgctc gagcggtttc gaccgaaggc gtttcccctg gtcgcaaacg
    11461 gcgatagatc tcgaccatgg cctcttcttt cgacactgtc cgttccattt cgagtgtgtc
    11521 gagaatcacc ggggtcgagg tggcagcgtc gaagaagatc actttaaatt cctcgatttc
    11581 gctctcgagg atcttctcga tgatctcggc ggtgaggcgc tggttttttt ccaccagctt
    11641 attcttcttg gtgtcgacca cttcgctcaa taccgcccgt ccgaccaatt cgcttggggc
    11701 aaccgggatc tctttgacgc cggccgcttt gagcttggcg atgatgcctt ttgtcagttt
    11761 gccgccttcc cgtaccagcg gttccttccc gctcttgtcg acgacctccg tcgaacagcg
    11821 catgccctga tggatttcgg gatcaagctt gcgcatgagc ttgcctttat tgactcggat
    11881 ttcttcgacg gggtagtaca ttttcagcag atcgtcgctc gaaaagccga atgccttcag
    11941 caatatcgtc gcgggcattt ttcgtcggcg gtcgatgcgg acgtacagga tgtcgcgtgc
    12001 atcgaattcg aaatccaacc atgaaccgcg atagggaatg atgcgggcgc tgtagagtac
    12061 tttgccgctg gcgtgcgtcc tgcccttgtc gtgcgtgaat gaagcacccg gcgaccggtg
    12121 caactggctg acgacgacgc gttctgttcc attgacgata aacgtcccgc gttcagtcat
    12181 gagcggcaat tcgccgacat agacctcctg ctcgcgcacg tcgaggacct tcttcttcgg
    12241 gcctttgtct tccttatcga acacgacaag ccttactcgc agtttgagcg gcacggcaaa
    12301 ggtcatgccc tgctcgaggc attcccgttc gtcatacttt ggcgtcccaa gtgtgtaact
    12361 tgtgaactcc aggatggctg tattgttgta gtccggaatc ggaaacacgc tggccaaggc
    12421 cgcttgcagt ccgtgatcct tccgccgttc gggctcgact tcgaattgaa ggaactcctc
    12481 gtacgaacgc ttctgaatct cgatcaaatc cggaatgtcg atgctggatc gaatacggga
    12541 aaaatccttc cgttcgacga actcttgtag agtcgtttcg ggcattccgc aatcctccac
    12601 tacatatggt tgggtgggca cccgtttgcg acgggtgccc gcagaactga acagaccgag
    12661 cttacttgat ctcgactttc gcgccgctct cctcgagctt tttcttcatg gcatcggact
    12721 cttccttggt tgcccccgtc ttgaccggct tgggagcgcc ttccacgaga tccttggctt
    12781 ccttgagtcc gaggttggtg agttcgcgca ccactttgat gacttgaatc ttcttgtcgg
    12841 caggtgccga cgcgaggatg acgtcaaatg ccgttttttc ttctgccgga gcggcagcgg
    12901 ctcccccggc ggcaggcgcc gcggcgacgg caaccggagc ggcagccgtg acgccgaacc
    12961 gagactccaa accttttacc agctcggcca aatccagcac gctcatgctt tcaatggctt
    13021 tgatcaattc ctcttgcgac accttcgttg cagtggctga catgtcccct tctcctttcc
    13081 gtttatcctg aatggctgca atgactctca caaacttggt tagcacggca ttcaacgtat
    13141 agaccacgcc gcgaatcgga ccttgcatgg ccgacaacag catggcaatc agcacttctt
    13201 ttttggggag tttggcaaca gcagccaatt ccgccggctg caggattttg ccctccaaca
    13261 cacctagcat gatacggatc ttttcttcgc gcttctccgc cccgataaaa tccctgagga
    13321 tcttcgtggg caacactgga tcgtcatagc cgatgacgac gcccgttggt ccttttaaat
    13381 gggtcttcag cccggccagt gtcgttccct cggatgcccg gatcgccagg gtgttcttca
    13441 tgaccttgta ttcggctttg gctccacgca attgtttacg gagctcggtc acctggttga
    13501 ccggaagccc ggcagattca gtgacaattg ctaatcgagc acggccgaat ttttccgtca
    13561 attccgcaac cgctgtggcc ttctcttccc ttttcatctc atcctctctt cttctttctc
    13621 tgtggctgcc gcccgacttc caatggatat agggatcgga tcaactccac tgctttgcca
    13681 gggcaatggc gtccaacttg accccgatgc ccatcgtgct cgaaatcgtc acgctcttga
    13741 gatagcgccc cttgcaggaa gcaggctttg ccttgacgac cgcctcaagg accgccgagg
    13801 cattgtcgta gagttgatcg ggctggaatg acactttccc caccggtacc tgaacaattc
    13861 cggccttttc aaccttatac tcaacacgac ccttcctgat ctcgcccacg gccttgccta
    13921 cttcgaaggt cacggttccg gttttcggat tgggcatgag cccacggggc cccaactgct
    13981 tcccaagctt accgaccgat gccatcaaat ccggcgtcgc aatggcacaa tcaaaatcca
    14041 tccaaccgcc tttgatcttt tccatgagat cgtctgatcc gacaaaatcg gcaccggcct
    14101 ggcgtgcctc ctgttccttt tcccctttgg caaacacgag cacccgcaat ttcttgcccg
    14161 tgccgtgagg gaga
//
LOCUS       DASTQY010000189         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_838828, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000189 DASTQY010000000
VERSION     DASTQY010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838828"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..366)
                     /locus_tag="VFR82_06270"
     CDS             complement(34..366)
                     /locus_tag="VFR82_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU4501240.1"
                     /translation="MRNGDYEYYEDDAMGWSAFVAGAFIGAGLALLFAPQTGTELRGM
                     LRNYAARAKDEAWERGRTAWDSAVERGKEYYEKGQEAVQDAGRSAREYVQSGKESIRE
                     GREGSHRG"
     gene            727..>2122
                     /locus_tag="VFR82_06275"
     CDS             727..>2122
                     /locus_tag="VFR82_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4501241.1"
                     /translation="MPSKRMYSADNRRSSVYLLQRAWPFIAPFRGQLTCLCFVVLLSI
                     PLSLLTPLPLTLAVDSVLGTKPLPVWLQAWLPPTVSSSPGSLLFFVCAAYIGVALLIH
                     VQSMVLWLLSSYTGERLIYAFRQRLFEHLQRICASYHEAHGPTDSVYRIQHDAASVKQ
                     IPIDAFLPFVKACCLLSGLAALMLLIDAEVAAVALTMLPVLFWLTHRCGRRLRQQWTD
                     VKSTETATVACAQEVLSASRLVKAFGREEHEQRRFLAHAMNWVRQHNALASICSGFDF
                     LFGMTITIGTAIALFIGISHVKGGRLSLGDLLLLMAYMAQLAGPLDTAAKKLTELQSH
                     LVGFRRALAILDTPPLVTDRPASTPLNKAKGHVAFRGVSFAYSSSAAVLREVSFHLPA
                     GTRVGIVGPSGAGKSTIVNLLTRFHDPTSGAVLLDGTDLREYRLTDLRKQFAIVPQDP
                     QLFSTTIAENIRYGD"
BASE COUNT          376 a          652 c          568 g          526 t
ORIGIN      
        1 gtgttcaagg tcgggacgga acggttggcg acttcagccg cgatgggagc cctctcggcc
       61 ctcgcgaata gattctttgc cgctttgcac gtactctcgt gccgagcggc cggcgtcctg
      121 gaccgcttcc tgacccttct cgtagtattc ctttccccgc tcaacggcac tgtcccaggc
      181 agtccgtcca cgttcccagg cttcatcctt ggctcgcgct gcgtagttcc gcagcatgcc
      241 tctcagttca gttccagttt ggggtgcgaa gagcaacgcc agtccagccc caatgaaggc
      301 gccggccacg aaagctgacc agcccatggc gtcatcttca taatattcgt aatcaccatt
      361 ccgcatcgtt tgcctccttt gttatgtggg ttcttgaaat gtgggttatc ggtctgctct
      421 gcgcattcct ttcccgtcgg aatgaagtgc aatccgcgga ccgcctcgac gccaggacgc
      481 tgggcctgat ttcaagaggt ttaagcgtcc aattcgatcc agcaggttgg gggaacgcgc
      541 cggcgcgaca gcatgtccgt caggtcggac agtatgtccg agctctaatt gtgactcgtg
      601 agtgcggaat gagaatcgtt tccaaatttt tacatcatat ttacgctacg tattcttcca
      661 acgattgcac gtccgagaac cggcgactac tatgcaccgt cctttgattg tgaacccact
      721 ttctcgatgc catcaaaacg gatgtattca gcagacaatc gccgctcttc cgtgtatctc
      781 ttgcaacggg catggccttt catcgcgcca tttcgcggac aactgacttg cctgtgtttc
      841 gtcgtcctcc tgtccattcc tctcagcctg ttgacacccc tgcccctcac cttagctgtt
      901 gacagcgtcc tcgggactaa gcctcttccg gtctggctgc aagcctggct tccacccacc
      961 gtctcttcat cgccggggag cttgctgttt ttcgtatgcg cggcctacat cggcgtcgcc
     1021 ctgctgattc acgtacagag catggtgctg tggcttttgt catcctacac cggggaacgc
     1081 ttgatctatg cttttcgtca gcgattgttt gaacatcttc agcgcatctg cgcctcttat
     1141 catgaggcac atggacctac cgactcggtc tatcgcatcc agcatgacgc ggcctcagtc
     1201 aaacaaatcc cgattgatgc gtttttgccg ttcgtcaagg cctgctgcct cttgagcgga
     1261 ctggcggcgt taatgttgct gatcgatgcg gaagtcgccg cggtcgcttt gacgatgctg
     1321 ccggtgcttt tttggctcac ccaccgctgc ggccgccggt tgcggcaaca atggaccgac
     1381 gtcaagtcga cggagacagc gacagtggcg tgcgcccagg aggtcttgag cgcttcgcgt
     1441 ctagtcaaag cgttcggacg agaagagcat gagcagcgac ggtttctggc tcacgccatg
     1501 aactgggttc ggcaacacaa tgctctcgcg tccatctgca gcgggtttga tttcttattc
     1561 ggcatgacga tcacaatcgg aaccgccatc gcgctgttca ttgggatcag tcacgtgaag
     1621 ggcgggcgtt tgagcttggg cgatctcttg ctgctgatgg cctatatggc gcaactggcc
     1681 ggcccgttgg acacagcggc caaaaaattg actgaattac agtcgcattt ggttggcttt
     1741 cggcgggcgc tcgccattct cgacacgccg cctctcgtca cagaccggcc tgcctccaca
     1801 cccttgaaca aggccaaagg gcacgtcgcg ttccgcggcg tgtctttcgc ctattcctct
     1861 tcagccgccg tgctcaggga ggtgtccttt caccttcccg ccggcacgag agtgggaatt
     1921 gtggggccga gtggcgccgg caaatctacg atcgtcaatc ttctcactcg attccatgat
     1981 ccgacctccg gcgccgtgct ccttgacggc accgacctgc gagagtaccg gttgaccgac
     2041 ctgcgcaagc agtttgcgat cgtccctcag gatccgcaat tgttttccac gacgatcgcc
     2101 gagaacatcc gttacggcga cc
//
LOCUS       DASTQY010000190         7114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1305335, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000190 DASTQY010000000
VERSION     DASTQY010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7114
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305335"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VFR82_06280"
     CDS             <1..188
                     /locus_tag="VFR82_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501242.1"
                     /translation="WLVATVEGYTTMTLNPRVLRLPLMKKTNEMRLVSSKAIPSVSHT
                     DPNCCRANVDKTPVDVS"
     gene            348..833
                     /locus_tag="VFR82_06285"
     CDS             348..833
                     /locus_tag="VFR82_06285"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4501243.1"
                     /translation="MTKPAKNTICLWYDRDAEEAARFYARTFPDSSVGAVLKAPGDFP
                     SGKKGDVLTVEFIVLGIPCLGLNGGPAFKHNEAFSFQVATVDQAETDRYWNAIVGNGG
                     EESACGWCKDKWGLSWQITPVALTKAITDPDPAAAKRAFDAMMEMKKIDIAAIEAARR
                     G"
     gene            complement(970..1296)
                     /locus_tag="VFR82_06290"
     CDS             complement(970..1296)
                     /locus_tag="VFR82_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501244.1"
                     /translation="MGRDEEGEILRAYGESLALVRLHHQLLQLSPYDAASREAWFDAA
                     KTLLATATNPSEGAGWLMTLAWAYKKHLGQGLPVSIIVGKLLGKRDRAALGLLPMVAN
                     MAGFAS"
     gene            1624..1917
                     /locus_tag="VFR82_06295"
     CDS             1624..1917
                     /locus_tag="VFR82_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501245.1"
                     /translation="MSRSNLDPLITFPDGSHLVISTQCSRQGEFSCALYTAIIAPDDS
                     ASFQMVSSHLEASTCLSAQEHAYGHAMQLYPRAVERMKKPPYLIWQGPPLKAG"
     gene            complement(2353..2724)
                     /locus_tag="VFR82_06300"
     CDS             complement(2353..2724)
                     /locus_tag="VFR82_06300"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4501246.1"
                     /translation="MKERYRKRVPLKAWVMFSNDGHCGQGRVLDFTVPGCQIESTEKV
                     IRGEYLELRILLPGQTALFTVRLAAVRWTKGTRFGAEFIRMDKSEQQTLDVFIADHFQ
                     RSTPDRHLRLTKEISERLQEG"
     gene            complement(2788..3441)
                     /locus_tag="VFR82_06305"
     CDS             complement(2788..3441)
                     /locus_tag="VFR82_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501247.1"
                     /translation="MGFLRSLFPLSLVLTGCLWGCSTVPPSLPPVRDSLSSPLFAPTA
                     EDAKRIVTLAHELDNRAQHCREASICEEVQFARGLVSLFESQEAARASFRRVIEENPS
                     SPLAGSSMLWLQLIGEAGSESAVEGPQKPSLHLMRQFVRDWMARELAEYTKPWQSITT
                     TQKTRVEPVEAVQALQKQVRERDRHIATLESKLEALKVIDQDDERRKRTIKVPATLP"
     gene            3664..4104
                     /locus_tag="VFR82_06310"
     CDS             3664..4104
                     /locus_tag="VFR82_06310"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4501248.1"
                     /translation="MASFKERHHDAPPLIVHWPLLYANKEFVGQGTLLNVSPWGCQVA
                     GTMPVARGMVLKLWISPAHRGDALYVKEARVLWARSNEFGIELREVDVQDHQWLMNFF
                     KECGHIFGQVARTYPEDGRASYIAPRFYGPEWSCPGGSMADDLT"
     gene            4196..4891
                     /locus_tag="VFR82_06315"
     CDS             4196..4891
                     /locus_tag="VFR82_06315"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HEU4501249.1"
                     /translation="MIPIALLLIFLGLTTGCASTLQAKPEHQSHAFKESAKGRIVTEA
                     VVPHIHGPSKVNWSGYDKVQLKPVIISNDFLSELSDEDREELGSLANSFHDMLTQRLS
                     ESYDLVDHAMPGSMLIEVAITHAERSWVAPAVLSKVSWQLQLLNGVLTYFRGKPAFAG
                     EITIEFTVHDAQTGVLLFTGTDRRVGGQNLFNREVFNSWGDVQNSFEYWTEQSTYQLC
                     LARRGTSCVQPKA"
     gene            complement(4935..5570)
                     /locus_tag="VFR82_06320"
     CDS             complement(4935..5570)
                     /locus_tag="VFR82_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4501250.1"
                     /translation="MSNSTTHSQDPSKRVQAVEQEATGTISERPAFLSDLFARESDFR
                     SFLRRRVADEALAEDLLQQSFMRAVQQEHQGERPEQVVAWFYRILRNATVDYYRARAA
                     DDRKMEAFIQELAVTGTDKTPALDELRPTVCACLQRLLPNLRPAYADLLNRIDLEGES
                     PVSVAKQLGVTPNNLTVRLHRARQALRKRLEESCGICTKHGCLHCTCDEAS"
     gene            5732..6046
                     /locus_tag="VFR82_06325"
     CDS             5732..6046
                     /locus_tag="VFR82_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501251.1"
                     /translation="MNKWIVSSLLSALSIFSAIGLQAAEQEEKPTPAIVETTGTRVTG
                     QLMHIEGWHYTIKDTRGEEVSFGVTSVTKDPDKVKEGDYIVAMIGDDRIALSVKNMKK
                     KN"
     gene            6305..6838
                     /locus_tag="VFR82_06330"
     CDS             6305..6838
                     /locus_tag="VFR82_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HEU4501252.1"
                     /translation="MTQQISDKHGREESHNHHNIHGTHSSHHQESASLNRTALMATVH
                     CLTGCTIGEILGMVIGTVLGWGNWPTVALAVVLAFFFGYTMTLWPLRKAGMAWGAALG
                     LAFASDTLSMTTMEIVDNAIMVVIPGAMDAGLGTALFWGSLILSLVVAGIAAFPVNRW
                     LIARGKGHALVHAHHGC"
     gene            6819..>7114
                     /locus_tag="VFR82_06335"
     CDS             6819..>7114
                     /locus_tag="VFR82_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501253.1"
                     /translation="MRIMDAEARMHQFKKYVAIVAMATIAFTLSGCSQFEQRDKRFYY
                     RALWNFALREQLKELDIEFNGVDFGHSNLYEHLLLTGAKDVEAIETNARKETLQ"
BASE COUNT         1678 a         1856 c         1930 g         1650 t
ORIGIN      
        1 cctggttggt ggcaacggtt gaaggctaca ccactatgac tctaaaccct cgcgttttaa
       61 gactgccact gatgaagaaa accaatgaaa tgcgtcttgt ttcttcaaag gcgataccat
      121 cagtttcgca caccgatcca aactgttgcc gggccaatgt tgacaagaca ccagttgatg
      181 tttcctgacc ttgtggtgta actgtcctgt tggttggcct ttctaggtca gattcaatcg
      241 agtaggcttc agtcataagc ataatgcaat cgatggcaag ctaatgtccg actgcaatcg
      301 cctggtttac tgcgtcgtca ggcggctcat cacgaggggg aagtgatatg accaagccag
      361 caaagaacac aatttgtctt tggtacgata gagacgccga ggaggcggcg cgattttacg
      421 ccaggacgtt tcccgattca tccgttggcg cggtactcaa ggcaccggga gattttccgt
      481 ccggcaagaa aggcgatgtg ttgacggtcg agtttatcgt attgggaatt ccctgcctcg
      541 gactcaacgg tggccccgcg ttcaagcaca acgaagcatt ctcgtttcag gtcgcaaccg
      601 tcgaccaggc tgaaaccgat cggtactgga acgcgatcgt gggtaacggc ggcgaagaga
      661 gtgcgtgcgg ctggtgtaag gacaaatggg gcttgtcctg gcagattacc ccggtggctc
      721 tgacgaaagc gataaccgat cccgatcccg ccgctgccaa gcgcgcgttc gatgcgatga
      781 tggagatgaa aaagatcgac atcgctgcga tcgaggcggc acgccgcggt taagatcaca
      841 tctcgaaacg tatattgcgg aagcgcagtg ggaacatcac tggaggacca aagccggcga
      901 ctcgtctgga ggagcttagc aactgtctag cgccacggag tccgcaggac ggagagggcc
      961 ttcggatagt caggacgcaa acccggccat gttagccacc atgggaagca atcccaatgc
     1021 cgccctatct ctttttccaa ggagtttgcc gacaataatg gagacgggaa ggccttgtcc
     1081 aaggtgtttc ttataagccc atgcgagcgt catcagccaa cccgctcctt ctgaagggtt
     1141 cgtggcggtg gccagaaggg ttttggcggc atcaaaccag gcttctcttg acgcagcatc
     1201 gtacggcgag agttgaagca attggtgatg tagccgaacc agggccaggg attctccata
     1261 tgcacgtaag atttcacctt cttcgtctct tcccatgagg gcctccctac ggagtagtaa
     1321 agcaagtcca ctgccgaccc catggcagta tgatcagcgc gggcttagta ctgtggtgtg
     1381 cagacagaac tgacgagatt gggatggaac gaccattttt tggcacggag actttatcgg
     1441 atgtccctcg tcgtctcttg catgtttgaa ccatccatcg aaaggctctt tgtcaccaga
     1501 tgatgacgaa tgcttgcgct cgcggagaga gtcatacacc atgcagagtc attactagac
     1561 gagtcggcta gaatgtacgg ataggttgtc actgcccgca tcgtatggca taagcataag
     1621 tctatgagtc gctctaatct ggaccctctc atcaccttcc cggacggctc acatctagtc
     1681 attagtaccc agtgttccag gcaaggcgag ttttcctgtg ccctttacac agccatcatc
     1741 gcgcccgacg acagtgcgtc ttttcagatg gtctcaagtc acctcgaagc atcaacctgt
     1801 ctcagcgctc aagaacatgc ctacggccat gccatgcaac tctacccgcg tgcggttgaa
     1861 cggatgaaaa agccgcccta tctgatatgg caaggtccgc ccctcaaggc ggggtgactt
     1921 ctatcctctc ggaaccttat tgacgcgcca agtcatgctg tgcgtccact gccctgccgg
     1981 acatctacgc tgaagggaaa agcttccagt ctcgtgcgat gggcgtgggc cacgagccaa
     2041 cggcgaatcg ctgctcctgc atgaaactga ctgcaacttg tggagatcac cgcatgctga
     2101 ggagcagcat gttgtaacga atgagtgctt ctctcgagaa gtgaagacga tagagattct
     2161 actcaaagac gcggtcgctg cgtaattgat gtgaccatgt atagctagcc catatatcgc
     2221 tcaatgcaca taataactaa ggcagctccg cccacctgga gaaacattgt ggttaaagaa
     2281 gaaatacgca tgctgcacct gcataccttt ctgtagatta agtcacccct gattgcctca
     2341 ttttgcatgg cttcatccct cctgaaggcg ttcggaaatt tcttttgtga gcctcaaatg
     2401 cctatcgggc gttgaccgct gaaaatgatc agcaatgaag acatcaagcg tctgctgttc
     2461 tgatttatcc atccgaataa actctgcacc gaatcgagtt cctttcgtcc atcggacagc
     2521 cgctaacctt acggtgaaaa gcgccgtttg accgggcagc aagatccgga gttctagata
     2581 ttcccccctt atgactttct ctgtgctttc gatctgacag cccggaactg tgaaatcaag
     2641 tacacggcct tggccacagt gtccgtcatt ggaaaacatg acccaggctt ttagtggaac
     2701 acgctttctg taacgctctt tcatatgcct cctcgctccc aacaggttcg gccatgagca
     2761 ctttcgacaa acgtggcagt agcaaggtta cggcaatgtc gcagggacct tgatggtgcg
     2821 ctttctcctc tcatcgtcct gatcaatgac cttcagagct tccagtttgg actcgagcgt
     2881 ggcaatgtgc cgatctctct cccgcacttg cttttgcagc gcctgcaccg cctcgacagg
     2941 ttcgaccctc gtcttctggg tcgtggtgat gctttgccac ggtttcgtat attcagccaa
     3001 ctcgcgtgcc atccaatcgc gaacaaactg cctcataaga tgaagtgagg gcttctgcgg
     3061 gccttcaacg gctgactcgc ttcctgcctc tccaatcagt tgcagccaca acatgctgga
     3121 gccggccaat ggactcgacg ggttttcctc gatcacccgc cggaaggatg cacgggcagc
     3181 ttcctgactt tcaaaaaggc tgacaagccc cctggcgaac tgcacttctt cacagattga
     3241 cgcttcacga cagtgctgcg cgcggttatc cagttcatgc gccaatgtga caatgcgttt
     3301 ggcgtcttct gccgttgggg cgaagagagg ggacgatagg gagtcccgga ctggtggtag
     3361 tgacggaggc actgtgctgc atccccagag acagccggta agtacgagcg aaagcggaaa
     3421 caagcttcgt agaaagccca tcatggcctc cacttggatt tgcagatttg ttaattcaag
     3481 cctagcagtc gaggaaatac acgccttcct caaaagtagg ggatcgaccc caccaggatg
     3541 ggggaggaaa gtgtctgcca gcctcctttg gagtacctcc ttgtgggctg gtgtgtccgc
     3601 agtcaccgct ctagatttag acaagaggct ctgactggac tggcttcacg aaaggagctc
     3661 aacatggctt cgtttaagga acgccaccat gacgcgccgc ccctaatcgt ccactggcca
     3721 ctgctctacg ccaataaaga atttgtgggt caaggaacgc tgctgaatgt ctccccctgg
     3781 gggtgccagg ttgccgggac catgcctgtc gccaggggca tggtgctgaa gctgtggatc
     3841 tcaccagcac atcgaggtga cgctctctac gtgaaagaag ccagggtact ctgggcacgg
     3901 tcgaacgagt ttggaattga acttcgcgag gtcgacgtcc aagaccatca atggctcatg
     3961 aactttttta aggagtgcgg tcatattttc ggacaggtag ctcgtaccta ccccgaagat
     4021 ggccgcgcct cctacatcgc tcctcgcttt tatgggcctg agtggtcctg tcccggtggc
     4081 agcatggcag atgacctgac ctagcccttc ccgactgtaa taatccctac cgacctccgt
     4141 ctctaggtta aacgaagcag tttgcaaccc ccaaaccagc aggaggaagt acgacatgat
     4201 ccccattgcg ttgttgctaa tctttctagg tctgaccaca ggttgcgcct ctacactaca
     4261 ggcgaaaccc gagcatcagt ctcatgcatt taaggagagc gcgaaaggca ggatcgtgac
     4321 cgaagcggtg gttcctcata ttcatggacc ttcgaaggtc aattggtccg gatacgataa
     4381 agttcagctg aaaccagtca tcatctccaa tgacttcctg tcggagttga gcgacgaaga
     4441 cagggaggaa cttggtagct tggcgaactc ctttcacgac atgcttactc agaggctttc
     4501 tgaaagctat gacctggtag accatgccat gccaggatcc atgctcatcg aggtggccat
     4561 cacgcatgcc gaacggtcat gggtcgcacc tgcggtcttg tccaaggtga gttggcaact
     4621 gcagctactg aatggcgtcc taacctattt cagaggaaag cctgcgtttg caggggaaat
     4681 cacgatcgaa tttaccgtcc acgatgcaca gacaggagta ttgctcttta caggaacaga
     4741 ccgacgagta ggtggacaga acctatttaa ccgcgaagtt tttaactctt ggggagatgt
     4801 tcagaatagc ttcgagtact ggacagagca gtcgacctat caattatgcc tggcacgccg
     4861 aggaacgagc tgtgttcaac cgaaagccta actatctgcg ataggtgaaa tgccacgacg
     4921 gggcctgcaa gttgctatga ggcctcgtcg caggtgcagt gaagacatcc gtgttttgtg
     4981 cagatgccac aggattcttc aagacgtttt cggagcgcct gtcgcgcccg atgcaggcga
     5041 accgtcagat tgtttggcgt cacgcctaat tgctttgcga cagagactgg cgattcccct
     5101 tcgagatcga tccgatttag aagatctgca taggcaggcc tcaggtttgg aagaagccgt
     5161 tgcaggcagg cacagacggt gggacggagt tcatcaagag cgggtgtctt atcagtcccc
     5221 gtgactgcta actcttggat gaaggcttcc atcttccggt cgtctgctgc gcgagcgcga
     5281 tagtaatcca ccgtggcatt ccgcaggatt cgataaaacc aggccacaac ctgctctgga
     5341 cgctcaccct gatgctcttg ctggacggct cgcataaaac tttgctgtag caggtcttcc
     5401 gcgagggctt cgtcggcaac tcggcgtcga agaaaagagc ggaaatctga ctcgcgagcg
     5461 aacaagtcag aaagaaaggc cggacgctca gagatggtgc ccgttgcttc ctgttccaca
     5521 gcctgaaccc gcttgcttgg gtcctgactg tgagtggtgc tgttgctcat ggggtgtcat
     5581 tctaccatca cagcctgcta gatcccatgt tctagactag gtctctgcag ggtattgtac
     5641 gcttttgaag tgcaccagaa tgttgccttg atagacgtcg aatgctagga caggggaatc
     5701 aaccaagagg attggagaaa ggaggaaggc catgaacaag tggatcgtgt catcattgtt
     5761 gagtgcgtta tccatttttt cagcaatagg gctgcaagct gccgaacagg aagagaagcc
     5821 gacccccgct attgtagaga caacggggac gcgggtgaca ggacaattga tgcacattga
     5881 agggtggcat tacacgatca aggacaccag aggagaagaa gtgagctttg gcgtcacatc
     5941 tgtcaccaaa gatccggata aggtgaaaga aggtgactat atcgtggcta tgatcggtga
     6001 tgataggatc gccttaagcg tgaagaatat gaaaaaaaag aattgaccta acagtgtgat
     6061 cgaaacaagt ttgctctcgg accaccgggc tttgcctcag tacaccggca ttgaccggac
     6121 tgcccacagc cctcctaaca ctctgtaaat caacgcatta aagcactgta agaattgaag
     6181 ccctcattcg tctgtagagg tgagcgttgg atggtccaac cctcacacac ggaggtctca
     6241 tatgatgcac gccgaatcag tttctgccac tctaccccgt ctgcaagatg aggaggcatg
     6301 ttgcatgaca caacagatca gcgacaagca cgggcgggaa gaatctcaca atcaccataa
     6361 tatacacggc acgcatagta gccatcatca ggaaagcgct tccctgaacc gcacggcgct
     6421 tatggctacg gtccactgtc tcaccggttg cacgatcggg gaaatccttg gaatggtgat
     6481 tggcacagtg cttgggtggg ggaactggcc aacagttgca ttggccgtcg tcctggcttt
     6541 cttttttggc tacacaatga ccctctggcc gcttcggaaa gctggcatgg cgtggggtgc
     6601 agcccttggg ttggcgtttg cctccgacac tttatcgatg acgacgatgg agatcgtcga
     6661 taatgccatt atggtggtga tccccggcgc gatggatgcc ggcctaggca cggcgctgtt
     6721 ttggggaagc ctgatccttt cgctggttgt agcaggcatt gcggcatttc cggtgaatcg
     6781 atggcttatt gcgagaggga aaggacacgc cctggtgcat gcgcatcatg gatgctgaag
     6841 ccaggatgca tcaattcaag aagtacgtgg cgatcgtggc gatggcgacg atcgccttca
     6901 ctctttccgg ctgcagccag tttgagcagc gtgataagcg cttctattac cgagcgctat
     6961 ggaatttcgc actccgcgag cagttgaaag aattagatat tgagtttaac ggggtcgatt
     7021 ttgggcattc gaatctctat gaacatttgc tcttgactgg tgctaaggat gtcgaggcaa
     7081 tcgaaacaaa cgctcggaaa gagacgcttc aatt
//
LOCUS       DASTQY010000191         3702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1047515, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000191 DASTQY010000000
VERSION     DASTQY010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047515"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1978
                     /locus_tag="VFR82_06340"
     CDS             <1..1978
                     /locus_tag="VFR82_06340"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU4501254.1"
                     /translation="RIDIVTAPNRTPEEVAASLRAVLSPSVVVQRPTQRTAQIEKMVG
                     AFQLNLAVLSWVGLLVGIFLVYNTMSFSVAQRRREIGIYRALGMTERRVAGLFLLEAG
                     LLGCLGGLLGGVAGLWMARSMVVLVSRTISDLYVPLGVTGTFSIVDAQSATAVAQGVV
                     VGIVVSMAGAVAPSIDASRTMTTKALAPGDYETSVALRSQRYLLGSLTLLIAGALCTL
                     GPPFRGLPILGYAATLCLLAGFALLAPVCILALRWSTQHPKQAPSLGTNLRRMAADHA
                     ARHPGRNAVTVSALLVGLAIMIGVVIMVRSFRETVEAWVNETVMADLIIAPPAWLHNK
                     QSGQASRTLPGTWLPLLQALNGVAAVDTYRDVEAEVEGQPVALISRDLRLHAERGRYL
                     MVEGEGATVLRRAADTGGVLVSEVLANRLGLQPGSLVSITTPKGPHTVSIEGVFYDYA
                     TDGGKMVMERSVYRRFWDDDRVTVFAIYVSPDADEERVVHSITEGFTGQELSVAPPII
                     IRNKDLRREILTIFDRTFVLTYVLEAIAVFVAVLGIVNTLVTAVMERRRELATLLAIG
                     ASRAQVERLVLWEAIYLGLIGALLGVMAGLALAWVLIAVVNKQSFGWTIRMTIPLEVL
                     VQAVLLAIVAAGVAGYLPARWAARQPLGEGLRDE"
     gene            complement(1986..3308)
                     /locus_tag="VFR82_06345"
     CDS             complement(1986..3308)
                     /locus_tag="VFR82_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU4501255.1"
                     /translation="MKHLTTGMFRRVVRGLSPTGFDLTVCVLLFSSWVAGCASTPTPL
                     TLPSMRIEDPAFRTSLEAFAGAPILGDNRVDILLNGEETFPALLDAIRSAHHTITFEA
                     YIFHEGKVADQIVGAFVERCKAGVRVAILLDAHGSDGLPERYIQNLRDAGCMLVSDFR
                     PLRFWSLERTNKRNHRRIMVIDGRVGFTGGYGVDDTWSGNGRTEGRWRETNVRLQGPI
                     VQSLQEAFVEHWREATSVLLGGKDYFPHPSVTVKDVPVLAQVVRSSPLQGNDAMYRVF
                     LQAISSARTSILISTPYLLPGEQLTEALLDAVRRGVRVRVLVPSVVKSSGVEFVTQAS
                     QREAFGALLEGGVQLHEYSPALLHTKMMIIDGTWATVGSTNFDNRSMAMNDELNVMFY
                     DRTIAKRLEEIFAADIAYSHKVSREQLENQEWLHRALGVLLSPFHAWF"
     gene            3352..>3702
                     /locus_tag="VFR82_06350"
     CDS             3352..>3702
                     /locus_tag="VFR82_06350"
                     /inference="COORDINATES: protein motif:HMM:NF021511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEU4501256.1"
                     /translation="MTASNDSLTTASKSLIPQVMLAWYALLFFSLAISPFDSRIWWGS
                     NILPVAFVAVLILTHRTFPLSNTSYVLITLWLTLHTVAVHYTYPKVPVGDWLDRWFDF
                     HRNHFDRIVHFSFGF"
BASE COUNT          724 a         1020 c         1133 g          825 t
ORIGIN      
        1 tcggattgac atcgtcaccg cacccaatcg aacgcccgag gaagtggccg cttcgttgcg
       61 agcggtgctt tcgccttccg tcgtggtcca gcggccgact cagcgtacgg cgcagatcga
      121 gaagatggtg ggggcgtttc aattgaacct ggcggtgctg agctgggtag gattattggt
      181 gggcatcttt ttggtgtaca acacgatgag tttctcggtg gcccagcggc ggcgggagat
      241 cggtatctat cgggcattgg ggatgacgga gcgacgagtt gccggtctgt tcctcctcga
      301 agccggactg ctcggctgtc tggggggact cttgggcggg gttgctggcc tctggatggc
      361 acgcagcatg gtggttctgg ttagtcgcac catcagtgac ctctatgtgc cgttgggggt
      421 gaccggaacg ttttcaattg ttgatgctca aagcgcgacg gccgtggctc agggtgtggt
      481 ggtcgggatc gtggtgtcga tggcaggcgc agtggcaccg agtatcgacg ccagccgaac
      541 gatgacaacc aaggcactgg cgccagggga ctatgagacg tctgtagcct tacgaagcca
      601 acgctaccta ttgggaagcc tgacgctctt gatagcggga gcgctgtgca ccttaggtcc
      661 tcccttcagg ggcctaccca ttttagggta tgccgcaacc ttatgtctct tagccgggtt
      721 tgcactcctg gctcccgtgt gcatcctggc actgcgatgg tcaacgcagc atccgaagca
      781 ggccccgtcg ctgggcacca atcttaggcg catggcggct gatcatgccg cgcgtcatcc
      841 cggccgaaat gccgtgaccg tatcggcatt gttggtggga ctggccatca tgatcggggt
      901 tgtcatcatg gtgcggagtt ttcgagagac ggtcgaggcc tgggtcaatg aaacggtcat
      961 ggcggatctc atcattgcgc caccggcttg gctgcataac aagcaatcgg gacaggcttc
     1021 gcgaaccttg cccggtactt ggttgcctct cctccaggcg ctcaatgggg tggcggcggt
     1081 tgacacgtat cgtgatgtag aggccgaagt ggagggccag cccgtggcac tcatatcacg
     1141 agatttgcgt ctccatgccg agcgcggccg gtatctgatg gtggaagggg agggggcgac
     1201 ggtgctcagg cgagccgcgg acacaggcgg cgtgctcgtg tcagaagtcc tagcgaaccg
     1261 gttaggcctt cagccaggca gccttgtcag catcaccacg cccaaaggac ctcacaccgt
     1321 ttccatcgaa ggcgtgtttt acgactatgc gaccgacggg ggaaagatgg tgatggaacg
     1381 aagcgtgtac cgccgttttt gggacgacga tagagtgacc gttttcgcga tctatgtctc
     1441 tccggacgcg gacgaagagc gggttgtcca ctcgatcacg gaaggattca ccggccagga
     1501 gttgtcggtg gcaccgccca tcatcatcag gaacaaggac cttcgccgag aaatcctcac
     1561 gatatttgac cgcacctttg ttttgacgta tgtgctggag gcgattgctg tcttcgtggc
     1621 cgtacttggc atcgtcaata ccctcgtaac cgccgtgatg gagaggcgcc gtgagctcgc
     1681 caccttgctg gcgatcgggg ccagcagggc gcaagtggaa cggctggttc tgtgggaggc
     1741 catctaccta ggattaatcg gcgcgctgtt gggtgtcatg gccggattgg cgctggcctg
     1801 ggtgctgatc gcggtggtca ataaacagtc gttcggatgg acgatccgca tgacgatccc
     1861 acttgaagta ttggtccaag ccgtgttgct ggcgatcgtg gcggccgggg ttgccggcta
     1921 cctacctgcg cgctgggcag ccagacagcc gctcggtgag ggcttgcgcg atgaataagc
     1981 ctgccttaaa accaggcatg aaacggactg agcaggacac ccagcgcacg atggagccat
     2041 tcctggttct ccaattgttc gcgcgagact ttgtgtgaat aggcgatgtc cgcagcgaaa
     2101 atttcctcta gccgcttcgc aatcgtgcga tcataaaaca tgacgttcag ctcgtcgttc
     2161 atggccattg aacggttgtc gaagttggtg ctcccgacag ttgcccacgt cccgtcgata
     2221 atcatcattt tcgtgtgcaa tagggctggg gaatactcgt gcagctgaac gccaccctcc
     2281 agcaaggctc cgaaggcttc tcgttgcgat gcctgcgtca cgaattcgac cccggagctt
     2341 ttcaccacac tcggaaccaa tacgcgaacg cgaacgccgc gtcggacggc gtccaacagg
     2401 gcctcggtca actgctcgcc cggcaagaga tagggcgtcg aaatcagaat agacgtgcgg
     2461 gctgaggaga tggcctgcaa gaatacacgg tacatggcat cgttgccttg cagcggtgag
     2521 cttcggacga cctgagccag aaccgggacg tctttgaccg tcacggacgg atgaggaaag
     2581 taatcttttc ctcccagcag cacgctagtg gcctcgcgcc agtgctcgac aaaggcttcc
     2641 tgcaggcttt ggacgatcgg cccttgcagc cgtacgttgg tttctcgcca ccgtccttcg
     2701 gttcgtccgt taccgctcca ggtatcgtcc actccatagc ccccagtaaa tccgacgcgg
     2761 ccatcaatga ccatgatccg gcgatggttc cgtttgttcg tccgttcgag gctccagaac
     2821 cgtaagggac ggaaatcgga tacgagcatg cagcctgcgt cacgaaggtt ctggatatac
     2881 cgttccggca gcccatcgga accatgggca tcgagcagta tggcgacacg aacaccggcc
     2941 ttgcagcgtt cgacgaaagc gccgacgatc tgatctgcca ccttgccctc gtgaaaaatg
     3001 taggcttcga acgtgattgt gtggtgagct gaccggatgg catcgagcaa ggctggaaac
     3061 gtctcttctc cgttgagaag gatatcgacg cggttgtcac cgaggatggg tgcccccgcg
     3121 aaggcttcca acgaggtgcg aaacgccgga tcctcgatgc gcatggacgg cagtgtgagc
     3181 ggagtcggtg tggatgcaca accggcaacc caagagctga agagcaagac acacaccgtc
     3241 aggtcaaatc ccgtaggtga aaggcccctc actacacgtc ggaacatccc tgtggtaagg
     3301 tgtttcatcc ccattccatg acctgtttgt ctgaaagtga ggcgtatggc tatgactgca
     3361 tcgaatgact cgttgacgac tgcatcaaag tccctgatcc cacaggttat gctggcctgg
     3421 tatgcgctgt tatttttctc attggcaatt tcgcccttcg attcaagaat ttggtggggt
     3481 tcgaatattc tgccagttgc gtttgtcgct gtgctgattc tgacccatcg tacgttccct
     3541 ttatcgaata catcctatgt cctcatcacc ctgtggctga cgctgcacac ggtcgcagtg
     3601 cactatacct atcccaaggt acccgtcgga gactggctcg accggtggtt tgactttcac
     3661 cgcaatcatt tcgatcggat cgtgcatttc agcttcggct tt
//
LOCUS       DASTQY010000192         7730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_733569, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000192 DASTQY010000000
VERSION     DASTQY010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733569"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFR82_06355"
     CDS             <1..340
                     /locus_tag="VFR82_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HEU4501257.1"
                     /translation="LYLSSERSLFRKAREALITRSLEHELTKERILELYLNVAEWGTG
                     VYGAEAASRHHFNKPARDLTEDEAAWLAAILPSPRRYDPLRKTHALVKRYERVLHRMA
                     QNSRHQIRMD"
     gene            348..2393
                     /locus_tag="VFR82_06360"
     CDS             348..2393
                     /locus_tag="VFR82_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4501258.1"
                     /translation="MGWRFIVCLSALCLLMSLPLAGDAAENPDADASFYTTDEVVISA
                     ARTPVAVGRSAAAVTVLTQEDILRSPYPGGHQLDDLLRSVPGVQPATLSSRYNHPTAQ
                     SVTVNGLGSRRALVLLDGIPLNDGFGGWINWGLIPNEIDRVEIVPGGASNLYGTWAMG
                     GLIHVITKRPSAGTGLTLDSQAGTLSTYQQALNARYGTDRFRFSVGYRWFHTNGIIPV
                     PSYQRGPVDRTNDSRHELVNGSLAWMPTQATTLDLSGSLFREDRTFGTTLSIASRTTG
                     NVAVGFRSDQDRAGLWEGRVFGQWQTFRNLTSQIIPSALNRQGEFQNDIQTIPSNDFG
                     GSLQWTGRLLDRDRLVLGTDARTIIAQSTDQLFSQTAFLGQTLARGQQLGWGLFGEWI
                     LPLTDHWTVTPGVRADWWKNYNARIETPAGTTVPRDNVVSVVNPKLSSLYQLSDTLRL
                     GASVYQAFRVPTLNELYRGFASGGFVFLPNDQLRPERLTGADVKLEGELLADRTLHWR
                     ISGHVDQVKDQILFVSQGPLAAQRQNVGKTLATGGTLELSYQLHPLVKLDLGYAYVDS
                     VIRNFPGSPDREGKRVPNVSRSQVTMGLKAGRVEFLEVTILGRYLSRQYADDANTQPV
                     ADFFVVDAALRHELRKGIQLMLNGENLTDRQYIATQTGPVKTLGSPLLIMGGVRAEF"
     gene            complement(2396..2836)
                     /locus_tag="VFR82_06365"
     CDS             complement(2396..2836)
                     /locus_tag="VFR82_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4501259.1"
                     /translation="MNDILFHLAFPIHDVNQAKRFYVEGLGCKLGRESATAVTFGLAD
                     HQLVAHVVEGSPSEQKGIYPRHFGLVFLSKHDWQALADRAKANGLRFFQQPRVRFPGT
                     AIEHHTFFLQDPSENLLEFKHYTRESAIFGERDFRAVGDASAAD"
     gene            complement(2833..3717)
                     /locus_tag="VFR82_06370"
     CDS             complement(2833..3717)
                     /locus_tag="VFR82_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HEU4501260.1"
                     /translation="MTRPHPLLLVHGGAGRRAMTRRQSSCLADALATGYAILEQGGPA
                     VVAVEVTIGFLEQSGLFNAGRGAKRQLDGVQRMDASIMEGAGLRAGAVASLEGFVHPI
                     TAARLVMEETDHVLLAGPLAGRFAQHFALERQRQPARRRQLEYERILSSKRRAKNRHG
                     TVGAVALDRSRTVAAGASTGGIEVMVPGRVGDTPLIGCGVYADNRCGAVSMTGQGEGI
                     IRLAVAKAICERLAAGKSPSTAAKMVLRELVSRIAGAAGTLVVAPDGRFTIAHVTLHM
                     AAGWWDGSTRPTVKGCWT"
     gene            complement(3714..4280)
                     /locus_tag="VFR82_06375"
     CDS             complement(3714..4280)
                     /locus_tag="VFR82_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501261.1"
                     /translation="MGTSIPARHPLRQLFGALTEKTFTEHLGWPDLNVTTYVTNLLVE
                     FTRTDELFKIRNRQGQPVDSVVDLLFESEVLLEAQSLDRERDVHQHIGDFTLFMAGLF
                     PEYLRRLKTAGLVYHKDFLVDYVKTGKRSYGIVAQMAAQPAQDERPLFEKLSDNFELC
                     VTGLGFVRSDLDRMKNPAYQQAKDLLLN"
     gene            complement(4344..4793)
                     /gene="dtd"
                     /locus_tag="VFR82_06380"
     CDS             complement(4344..4793)
                     /gene="dtd"
                     /locus_tag="VFR82_06380"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEU4501262.1"
                     /translation="MKAVIQRVTRASVEVEGRVVGSIGPGLLVLLGVAKGDGDADCRC
                     MVEKLRGLRIFADEAGKMNRSLAETGGGILLVSQFTLLGNTKSGRRPGFEDAAPPDEA
                     RQWYERVSEELKVQGTQVETGVFAAHMKVELVNDGPVTFLLDSRSGF"
     gene            complement(4790..5320)
                     /locus_tag="VFR82_06385"
     CDS             complement(4790..5320)
                     /locus_tag="VFR82_06385"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU4501263.1"
                     /translation="MTRALIFKSSWGWMGVAESKEGLSAIVLPQTSKASVTAALEKGG
                     AGFDASSSSSLLREARKQLMEYLAGRRTSFDLPLDLSRGTAFQQRVWKKLGAIPYGQL
                     WSYRGLASRVGGVQYARAVGGAVGANPLPIVLPCHRIVAQDTGLGGFSCGLPAKRKLL
                     ALEGSLPRVRHADRGR"
     gene            complement(5330..6940)
                     /gene="murJ"
                     /locus_tag="VFR82_06390"
     CDS             complement(5330..6940)
                     /gene="murJ"
                     /locus_tag="VFR82_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEU4501264.1"
                     /translation="MSDTATALPKTSQSENHSLVKAAGVIGVATFSSRILGFIRDMVL
                     ARLFGATPAADAFYVAFRIPSLLRELFAEGSMSAAFIPVFTEYHSLKSRREAWELASA
                     VFTTLLTIVTLVTLLGILIAPWLVQALAPGFHASPEKLALTTVLARIMFPYLLFISLA
                     SLAMGILNSVRAFAVPAYAALFLNVCIIGCALFLSPQLEEPIVGVAIGVVAGGAAQFI
                     MQIPSLKARGFLFGIRFEPGHPGVKQIGRLMLPSLLGLSVTQVNLTISTILASFITGA
                     PTYLFYGMRLVQFPLGIFGVALAMAILPTLSAQAARGAIEELRTTLGFGLRMIVFIMF
                     PAMLGLIFLRTPIVHVFFEHGTFTAHDTAETAMALLCYAVGLWAFGGVRIIVAAFYSL
                     QDTKTPAISGAIAVAANILFSLLLMSPLGAAGLALATALAGMVNGIILVALLNRRLGG
                     VEWESVGRSAGRVLVACIPLVLSCLWVAGAQVWTHEGEWAAKSVMLTVAIGLSVSGYL
                     GVHALMRSEELDLVWSMVKKRFGRLNGR"
     gene            complement(6990..>7730)
                     /locus_tag="VFR82_06395"
     CDS             complement(6990..>7730)
                     /locus_tag="VFR82_06395"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU4501265.1"
                     /translation="GADGAAVRLTPEDSVVAAVISVEEVPVATGRLVPLTDEEKGRIS
                     DAVRAAERLTSAEIVPMLVARSGLYHDAQHRAGLALALVVLTGLLMSEAFWLSWGWHA
                     ANAAWLIVSTLVGYVIGSWLGTFDPVVRAVTSTDRLRSKVQLRAERAFAQHSLFRTRD
                     RTAVLLLVSLMERHVYVLPDSGIGPRIAAAQWNEVVEAVVTRLKAEDIAGGFCAGIER
                     CGAFLALAYPASSNDNPDELSNRLVQEP"
BASE COUNT         1668 a         2424 c         2222 g         1416 t
ORIGIN      
        1 tctgtatctc tcttccgagc ggtcgctctt ccgaaaggct cgcgaagcgc tgatcacgcg
       61 ctcactggaa catgagctga cgaaagagcg catcctcgag ttgtacctca acgtggccga
      121 gtggggcacc ggtgtctacg gtgccgaagc ggcctcccgg caccacttca ataaaccggc
      181 acgtgacctc accgaagacg aggccgcttg gctggccgcc attcttccgt caccgcgccg
      241 ctacgatccg cttcggaaaa cacatgcgct cgtcaagcgc tacgagcggg tgctccatcg
      301 aatggcccag aactcacggc atcagatccg gatggactag ggcctgcatg gggtggcggt
      361 tcatcgtgtg cctgtcagcc ctatgcctgc tcatgagtct gcctctcgcc ggtgacgccg
      421 ccgagaaccc cgacgctgat gcttccttct ataccaccga cgaggtcgta atcagtgcgg
      481 ctaggacgcc ggtagccgtt gggcgcagcg ccgccgccgt gacagtgctc acccaagagg
      541 acattctgcg gtctccatat ccgggcggcc atcaactcga tgatctgctg cgttcggtgc
      601 ccggcgtaca gccagccacg ctgagcagcc ggtacaacca ccccacggcg caatccgtca
      661 ccgtcaatgg actcggctcc cggagggcac tcgtcttgct cgatggcata ccgctcaacg
      721 acgggtttgg agggtggatc aattggggcc tgatcccgaa cgaaatcgac cgggtggaaa
      781 tcgtgcccgg aggcgcgtcg aacctgtacg gcacgtgggc gatgggcggg ctcattcatg
      841 tcatcacaaa gaggccgagc gccgggaccg gattgacgct ggacagccag gctgggacgc
      901 tcagcacgta tcaacaagcc ctgaacgcgc ggtacggcac cgatcgtttc cgtttttccg
      961 tcggataccg gtggtttcat acaaacggca ttatccccgt tccctcatac cagcgaggac
     1021 cagtggatcg cacgaacgat tcccgccatg agttagtcaa tggcagcctg gcctggatgc
     1081 cgacgcaggc caccaccctc gacctgtcgg gtagcctatt tcgggaggac cggacgttcg
     1141 gcacgacgct cagcatagct tcgcgcacga ccggcaacgt cgccgtcgga tttcgcagcg
     1201 atcaggaccg tgcaggactg tgggaaggca gagtcttcgg tcaatggcag acctttcgga
     1261 acctgacctc ccagatcatt ccatcggcgc tcaaccgtca gggggaattt caaaacgaca
     1321 ttcagacgat tcccagcaac gacttcggcg gatcgctcca atggacggga cggctgttgg
     1381 accgggatcg actcgtattg ggaaccgacg cacgcacgat catcgctcaa tccaccgacc
     1441 agctgttctc gcaaacggcg tttctcggtc aaacgctcgc ccgtggccag cagcttggtt
     1501 ggggcctctt cggcgaatgg attcttccgc tcaccgacca ttggaccgtg accccgggag
     1561 tgcgcgccga ttggtggaaa aactacaatg cccgcattga gactccggcc ggtacgaccg
     1621 ttccccgcga caacgtcgtc agcgtcgtta atccgaaatt gtcttctctg tatcaactta
     1681 gtgacacgct gcgactgggt gcttccgttt atcaagcgtt ccgagtgccg accttgaatg
     1741 agctgtatcg gggatttgcc tcgggcggat ttgtctttct cccaaacgat caactccgtc
     1801 cagaacggct gaccggagcg gacgtgaagc ttgaaggaga gctcctcgcc gaccggacgt
     1861 tgcactggcg catctccggc catgtggatc aagtcaaaga tcagattctg ttcgtgtctc
     1921 aaggcccgct ggcggcgcaa cgtcaaaacg tcggcaagac gctcgccacc ggagggacac
     1981 tggagctgtc ctaccagctt catcccctag tcaagcttga tctcggctat gcttatgtcg
     2041 attccgtgat ccgcaatttt cccggcagtc ctgatcggga aggaaagcgg gttcccaacg
     2101 tctcgcgaag ccaagtcacg atgggactga aggcgggtcg tgtcgagttt ttggaagtga
     2161 cgatcctggg ccgttacctt tcgcgtcaat atgcggacga tgccaatact cagccggtgg
     2221 ccgatttctt cgtggtggac gccgcgttgc gacacgagct ccgcaagggc attcagctga
     2281 tgttgaatgg agaaaatctc accgaccgac agtacatcgc cacgcagacc ggtcccgtga
     2341 agacgctcgg cagtccgttg ctgatcatgg gcggggtacg agccgagttc taagctcagt
     2401 cggcggcgga ggcatcgccc acagcacgaa aatcacgctc gccgaaaata gcggactcgc
     2461 gggtgtaatg tttgaactcg aggaggttct cggacgggtc ctgaaggaag aacgtatggt
     2521 gctcgatggc agtacccgga aaccggactc gcggctgttg aaagaaccgc agcccgttgg
     2581 ctttcgcccg atccgccaat gcctgccaat cgtgtttcga gagaaacacc aagccgaaat
     2641 gacgaggata aatacctttc tgttcgctgg gagacccttc tacaacatgg gcgaccagtt
     2701 ggtggtcggc cagtccgaac gtcacggcag tggcggattc gcgtccgagc ttgcacccga
     2761 gtccctcgac atagaaccgc ttggcctggt tgacgtcgtg gattgggaaa gccaagtgaa
     2821 acaggatgtc attcatgtcc aacaaccttt gactgtcggt cgcgtcgacc cgtcccacca
     2881 tccggcagcc atgtggaggg tcacatgggc gatcgtgaag cgcccgtccg gtgcaactac
     2941 caatgtacca gccgcgcccg cgatccgcga gaccagttcg cgcaagacca tcttggccgc
     3001 cgtcgacggc gactttcctg ccgcgagccg ctcacagatc gccttggcaa ccgccaaacg
     3061 gatgatgcct tcgccttggc ccgtcatcga caccgcgccg cagcgattat ccgcatagac
     3121 cccgcaaccg atcaagggcg tgtctcccac ccggccgggc accatgacct cgatccctcc
     3181 agtcgaagct ccagccgcga cagtgcgaga gcggtccagg gcaacggcac cgacggtccc
     3241 atgacgattc ttcgcgcgtc gcttgctgga aagtattcgt tcatactcga gttgacgacg
     3301 tcttgcaggc tgacgctgtc tctcgagagc aaaatgctgc gcaaatctgc cggcgagggg
     3361 ccccgctaac aacacgtggt ccgtctcctc catcaccagc ctggctgctg tgattggatg
     3421 gacgaagcct tccagcgaag ccaccgcacc ggccctgaga ccggccccct ccattataga
     3481 ggcgtccatt cgctggacgc cgtcgagttg ccgcttcgcg ccgcggcccg cattgaagag
     3541 accggattgc tcaagaaagc cgatcgtgac ttcgaccgcc actactgccg ggcctccctg
     3601 ctcaagaatc gcatagccgg tggcaagcgc gtcggcaagg catgacgact ggcgcctggt
     3661 cattgcgcgc cggccggcac cgccgtgtac cagaagaagg ggatgaggac gggtcaattg
     3721 aggaggagat ccttggcctg ttggtaggcc ggatttttca tacgatcaag gtcggaacga
     3781 acaaatccca gccccgtgac gcaaagctca aagttgtcag aaagtttttc gaaaagaggc
     3841 ctttcgtcct gggcaggctg ggccgccatc tgcgcaacga tgccgtagga acgtttcccc
     3901 gtcttcacgt aatccaccag aaaatcctta tggtagacca agccggcggt cttcagccgg
     3961 cgcaggtatt ccggaaagag cccggccatg aacaaagtga agtcgccgat gtgctgatgg
     4021 acgtcccgtt cccgatccaa tgactgcgcc tcaaggagca cctcggattc gaacagcagg
     4081 tcaacgacag aatcgaccgg ttggccctgg cggtttctaa tcttaaacag ttcatcggtc
     4141 cgggtgaact caacgagcag gttggtcacg taggtggtca cattgagatc gggccagccg
     4201 agatgttccg tgaaggtttt ctcggtcaag gcgccgaaaa gctgacgcag cggatgtcga
     4261 gcggggattg acgttcccat ctctcctctc actggatctc aggcagtcaa acctattata
     4321 gcagagtctg atccgaagac cgcttaaaag ccgctccgac tgtctaacag aaaggtgacg
     4381 gggccgtcgt tcaccaactc gaccttcatg tgggcggcaa atacgcccgt ctcgacctgg
     4441 gtcccctgta ccttcaattc ctctgaaaca cgctcatacc attgtctggc ttcatcgggc
     4501 ggcgcggcat cctcaaagcc agggcgccgt ccgcttttcg tattgcccaa gagggtgaat
     4561 tgggacacca ggaggatgcc gcctcccgtc tcagccagcg accggttcat tttgcccgct
     4621 tcatcggcaa agatacgcag gccacgcagt ttctcgacca tgcaccggca atctgcatct
     4681 ccgtcgccct tcgccacacc aagcaggact aacagtcccg gcccaattga gccgacgacc
     4741 cgcccctcca cttccaccga tgcgcgagtc accctctgga tgaccgcttt catcggcctc
     4801 gatccgcatg tcgaacccgg ggcagcgatc cttccagggc caataacttg cgtttggccg
     4861 gaagcccaca tgaaaatccg ccgagcccgg tgtcctgggc aacgatccga tggcacggta
     4921 agacgatcgg caagggattc gcgccgaccg ccccacccac cgctctcgca tactgtaccc
     4981 cgccgacccg tgaggccaga cctcggtaag accatagttg accatagggg atcgccccta
     5041 gcttcttcca tacgcgctgc tggaaggcgg ttcctcgcga gaggtcaagt ggaagatcga
     5101 aggacgtccg cctcccggcc agatactcca tcaattgctt gcgtgcttcc cgtagaagag
     5161 aagacgaaga tgaggcgtcg aagccggcac cccccttttc caaggccgcc gtcacggacg
     5221 cctttgaggt ctgcggcagc acgatggcgg aaagtccttc ttttgattcg gccacgccca
     5281 tccaccccca gctcgactta aagattaacg cgcgtgtcat cagatcctcc tagcggccgt
     5341 tgagacggcc gaaccttttc ttgaccatgc tccacacgag atcgagttct tcggaccgca
     5401 tcagcgcatg gacgccgaga tagccgctca cactcagtcc gatggcgacc gtcagcatca
     5461 cggattttgc cgcccactcg ccctcatgcg tccagacctg ggcgccggcc acccaaaggc
     5521 aggacagcac cagaggaatg caagctacga gcacgcgtcc cgccgaacgg cctaccgatt
     5581 cccactcgac accaccgaga cgacggttga gcaaggcgac caaaatgatc ccgttcacca
     5641 tcccggccag cgcagtagct aacgctagac cagcggcacc caagggagac atcagaagca
     5701 aggagaagag aatgttggca gctacggcaa tggcgccaga gatggcagga gttttggtgt
     5761 cctgaagcga gtagaacgcc gccacaatga tgcgtacgcc tccgaatgcc cagaggccca
     5821 cggcataaca caacaaagcc atggccgttt cggcggtgtc atgagccgta aacgtcccgt
     5881 gctcgaagaa cacgtgcacg atcggcgtcc gcagaaaaat cagcccgagc atcgccggaa
     5941 acatgatgaa cacaatcata cgcaatccga atccgagcgt ggtccggagt tcctcgatgg
     6001 cgccgcgagc cgcctgggcc gagagcgtag gcagaatggc catggcgagc gccacgccga
     6061 agatgcccaa cggaaactgg accaaccgca tgccgtagaa caaatacgtc ggcgcgcctg
     6121 tgatgaagga tgcgaggatc gtactaatgg tcaaattgac ctgtgtcacc gacaggccga
     6181 gcagcgacgg cagcatcaac cggccgatct gcttcacgcc gggatggcct ggctcaaacc
     6241 ggatgccgaa caagaatcct ctcgctttaa ggctggggat ttgcatgatg aactgagcag
     6301 ctcctccggc cacgacgccg atcgccacac cgacaatcgg ttcctctaat tgcggggaca
     6361 gaaaaagcgc gcacccgatg atgcacacgt tcaggaagag cgccgcataa gcggggacgg
     6421 caaaggcccg gactgaattc aagatcccca tcgccaacga ggcgagactg atgaaaagga
     6481 gataaggaaa catgattctt gccagtaccg tcgtgagcgc cagtttctcc ggcgacgcat
     6541 ggaagcccgg tgcgagagcc tggacgagcc agggcgcgat gagaatgccg agcaatgtca
     6601 ccagggtcac gatcgtcaac agcgtggtga agaccgcgct cgccaattcc cacgcctcac
     6661 gtctggattt gagggagtgg tattccgtaa acaccggaat aaaggcggcg gacatcgacc
     6721 cttccgcgaa tagttcgcgc agcagacttg gaatacggaa ggcgacgtag aatgcgtcag
     6781 cggccggcgt ggcaccgaac aagcgcgcga gaaccatgtc acggatgaaa ccgaggatgc
     6841 ggctcgaaaa ggtggcaacc ccaatgactc ccgcggcttt caccagtgaa tggttttcgc
     6901 tctgggatgt tttcgggaga gccgtggcgg tatcagacat ctgggcggat tctaacggaa
     6961 cgatggcgcg cagtctattg aacagcgcgt tatggttcct gcacgagtcg gtttgagagt
     7021 tcgtcgggat tgtcattgga cgaggcagga tacgcgagcg ccaggaaagc cccgcagcgt
     7081 tcgatgccgg cgcagaatcc gccggcgatg tcctccgctt tcagcctcgt cacgacggct
     7141 tccaccacct cgttccattg tgccgccgcg atgcgcgggc cgattccgga atccggcaat
     7201 acatacacat gccgttccat caacgagacc aggagcagca cggccgtgcg gtcccgagtc
     7261 cgaaagaggc tgtgttgtgc gaaagcgcgc tccgccctca gttgcacttt gctgcgaagc
     7321 cggtcggtcg aagtgaccgc ccggacgacc ggatcgaacg tgccgagcca cgagccgatg
     7381 acatacccca ccagggtcga gacgatgagc cacgcggcat tggctgcatg ccaaccccat
     7441 gacaaccaga aagcctcgct catcagcaag ccggtgagca ccaccagcgc caaggccagg
     7501 ccagcgcgat gttgcgcatc atgataaagt ccggagcgtg ccacgagcat cggcacgatc
     7561 tctgcgctcg tgagccgttc agcggcacga actgcatcgc tgatgcggcc cttctcctcg
     7621 tcggttaatg gcaccagcct accagtcgcc actggcacct cctccaccga aatcaccgcc
     7681 gccaccactg aatcctccgg ggtcaaacga accgccgctc catccgcccc
//
LOCUS       DASTQY010000193         7201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1225230, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000193 DASTQY010000000
VERSION     DASTQY010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225230"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFR82_06400"
     CDS             complement(<1..307)
                     /locus_tag="VFR82_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501266.1"
                     /translation="MNEKTIRRSLKTIGTLYGLYGAVALMTLAGSIWTGMKLGREGIS
                     QFIDIANIPWTLVLLGHLLLPFMAFVCFGIAYAFLNLKNWGHQLAIALNFVMCVLLMC
                     "
     gene            complement(559..636)
                     /locus_tag="VFR82_06405"
     tRNA            complement(559..636)
                     /locus_tag="VFR82_06405"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(598..600),aa:Glu,seq:ttc)
     gene            769..1251
                     /locus_tag="VFR82_06410"
     CDS             769..1251
                     /locus_tag="VFR82_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501267.1"
                     /translation="MQGRNLNWFVTNVICCFLLTACAHDTFQQRADVIKDHTEAFYSH
                     LKANRVESAVRENEQIEAMASEMGRTVRKRASLQGATPVEREFALMKTAKEAAATNWL
                     ALGQYFAIKKQYPQARATYQRILNTYDHQTDRPYREQAARAIEDLNILDPPSASATNP
                     "
     gene            1248..1796
                     /locus_tag="VFR82_06415"
     CDS             1248..1796
                     /locus_tag="VFR82_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501268.1"
                     /translation="MSVQNSRYVLSHVFALSLLFLGGCAHSIHVAPVAPSVSTSPIDH
                     ALQVQVPLLALQGADRMPGVIMLVWPVKDLRQAVLEYARQRRTFTEVLDEHGDFTLTV
                     RTWLWLRSREAYRYIVHLEADLGPTGQPPIKSYVVEEETVGSRIRWVTASDQAPIAAA
                     VQAVLDELFDHIEQDAALFRKK"
     gene            complement(1812..2024)
                     /locus_tag="VFR82_06420"
     CDS             complement(1812..2024)
                     /locus_tag="VFR82_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501269.1"
                     /translation="MKRFPWTSSVASEEWLGAESHLPNLKHELRKQNHRGWNTAEVVV
                     AGALFTAGLTLGTLAALWLTRSRQTR"
     gene            complement(2079..2774)
                     /locus_tag="VFR82_06425"
     CDS             complement(2079..2774)
                     /locus_tag="VFR82_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEU4501270.1"
                     /translation="MAELSVLNQPAKPLPKDRLPVPVQLAAGLEKISLALKSRTWRRE
                     GQAGLGPLQRQVLTLLRSKADHSAQVSAIAQELVVRLPTASEAVATLERKRLVKRFRS
                     HRDGRIVNVELTARGARACAPAVGIPNLLAEAASALTSTEQVAFLKGLMKVIRTLQQQ
                     GEISVARMCVSCRFFRPHQYDDAGQPHHCDYVNAPFGDYALRLDCAEYESASISQAKE
                     SWDRFNQGRTAIG"
     gene            complement(3277..4107)
                     /locus_tag="VFR82_06430"
     CDS             complement(3277..4107)
                     /locus_tag="VFR82_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4501271.1"
                     /translation="MTGDYSAADLCRILRLDGSQLRACLRAALLPLPGVNRSCTYSFQ
                     NLVVLRTTKGLTEAGVSVAKIRKVLECLERQLEEDTSIASVKIYASGKRVVVWDGASR
                     WQPDSGQFLLNFETAQMLPPRSLRPAPQVQRNERETAERWFQRALTLVEDSPEEARQA
                     YQEAIRLHPGFTDAHINLGLLNHNLGLLADAESCYRRALEHEPKLALAHFNLGVLLED
                     RHQKSAAISAYKEALLHEPDFFEAHCNLAQLYEKLGRKRDAFRHYAAANRLRGRGISQ
                     "
     gene            complement(4104..4958)
                     /locus_tag="VFR82_06435"
     CDS             complement(4104..4958)
                     /locus_tag="VFR82_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEU4501272.1"
                     /translation="MGRRLETAHISFGLVTIPVGIYSAIDEQDIHFNQLHAVCGSRIK
                     QQRFCPVCQREVKYEELTKGYEITKDEYVRIDQEELDRLQAAESEAMQILEFVPLSAV
                     DPIYFESTYYLGVEKQGEKAYQLLAQAMEDMQRVALAKYVWRGKDSLHVIRSVDGRLL
                     LHRMYYHDEIRPFIEVPKSDQKPASAELKLATQLIDSISTDRFDAGAYQDEYRQRVIE
                     LIEQKSKGKTITLSPKAAAPVTDVVDLMQRLKESLANTAQKKTARSRALPAQTAREPA
                     KKASSGRR"
     gene            complement(5126..5656)
                     /locus_tag="VFR82_06440"
     CDS             complement(5126..5656)
                     /locus_tag="VFR82_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU4501273.1"
                     /translation="MSLFGSSNPVFEMLEKDHAKVKDLFEQFEDAQDARSRERIIQET
                     LFELEVHAKLEESLIYPAIRKKLDEEDIMDEALEEHHVAHVLMNELKRMRPSDDRYHA
                     KFKVLGENIKHHVKEEEGTMFPKAEKADLDWERLEQKAREQKEALMARKINGTKRDRA
                     KKPRRRTARRTLRRAA"
     gene            complement(6081..6156)
                     /locus_tag="VFR82_06445"
     tRNA            complement(6081..6156)
                     /locus_tag="VFR82_06445"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6121..6123),aa:Cys,seq:gca)
     gene            complement(6664..6828)
                     /locus_tag="VFR82_06450"
     CDS             complement(6664..6828)
                     /locus_tag="VFR82_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501274.1"
                     /translation="MRNVPTGIAVWGLRVEDEDWSNDLHAERELFERDQSQFLKEYRV
                     MSIDRIRVLL"
BASE COUNT         1689 a         1971 c         1841 g         1700 t
ORIGIN      
        1 ggcacatcaa aagtacacac atgacaaaat tcaacgctat cgccagctgg tgtccccaat
       61 tttttaaatt tagaaacgca tatgcgatac cgaagcagac gaaggccata aacggcagca
      121 acagatgtcc gagcagcaca agcgtccaag ggatattcgc tatgtctatg aattgtgaaa
      181 tcccctctct cccaagcttc atcccagtcc aaatagaacc tgcaagcgtc atcagggcaa
      241 cagctccgta taacccatac aaagtcccga tggttttaag tgatcgacga atggtctttt
      301 cgttcataag ccctggatag atggatgcca gactataaga gaagtataga cggggaaggc
      361 aggaacgcca ggaaaactga ggtctttgtg tctcgccagg aagtcgaaag attgacctgc
      421 ggtttatcag tccgcttcgc ttccgacttc tgttaacaga gcaagccgct aagaaagtcc
      481 cgtcatttac acggcaacca gtaggttttg atcaagagtg gagctagaag ctgcaggaat
      541 gttctacgta gttctacctg gtgtccccaa cgggattcga acccgtgttg ccggcttgaa
      601 aggccggcgt cctaggccag gctagacgat ggggacctga ggtgagcaag aagggaaaca
      661 cataccacac tcaagacggg catgtcacta caacccacac gaaggtcagt cgatatttca
      721 tgtttgatca ggtaggatgg gggcctcgtc ggctgagggt cgagggtcat gcaaggaaga
      781 aacctgaact ggtttgtcac caatgtgatc tgctgcttcc tgctcactgc ctgcgcacat
      841 gacacgtttc agcagcgtgc cgatgtcatc aaggatcata ccgaggcctt ctactcccat
      901 ttgaaagcca accgtgtcga gtcagctgtg cgggaaaacg aacagattga agccatggca
      961 agcgagatgg gccgtacggt tcgaaagcgc gcctcgctgc aaggggcgac tccagtcgag
     1021 cgagagtttg ccttaatgaa gacagccaaa gaagctgccg caaccaattg gctcgctctc
     1081 ggccagtact tcgccattaa aaagcagtac ccacaggccc gagcgaccta ccaacgcatc
     1141 cttaatacct acgatcatca gactgaccgc ccctaccgcg agcaagcagc ccgcgcaatt
     1201 gaagacctta atattctcga tccaccgtcc gcctcagcga caaacccgtg agtgtgcaga
     1261 acagtcggta tgtgctgagc catgtgtttg ccttgtctct gctgtttctg ggcggctgtg
     1321 cccacagcat tcatgtcgca cccgttgcgc cttccgtgtc gacctcgccc atcgaccacg
     1381 cgctgcaggt acaggtcccg ttgcttgcac tgcaaggcgc agaccgaatg ccaggagtca
     1441 tcatgctcgt ctggcctgtg aaagaccttc gtcaggctgt gcttgagtat gcgcggcaac
     1501 gtcgcacgtt caccgaggtt cttgatgaac acggggattt cacgttgaca gtccggacat
     1561 ggctctggct gcgttcccga gaggcatacc gctatatcgt ccatctcgag gcagacctcg
     1621 gaccgaccgg tcagccaccc atcaaatcct atgtggtcga ggaagaaaca gtgggttctc
     1681 gcatccggtg ggtcacggca tcggatcaag ctccaatcgc agccgccgtg caggcagtcc
     1741 ttgacgagct attcgatcat atcgagcagg atgcggcgct gttcagaaag aagtaacgag
     1801 cgagagcagc gctagcgagt ttgtcgcgat cgagtcaacc agagcgcggc aagagttccc
     1861 aaggtgagtc ccgctgtgaa caaggccccc gccaccacga cctcggcggt attccagcca
     1921 cgatgattct gttttcgcaa ttcatgcttg aggttcggca aatgcgattc agcgccgagc
     1981 cattcttctg aagcgacgga agatgtccag gggaaacgtt tcattcctcg gtccttttcc
     2041 atttgatgaa ccacaacttt tctcatacag cctagcgatt agccgatcgc tgtgcggccc
     2101 tggttgaatc ggtcccacga ctccttcgct tgcgagatgg aggcggactc atactccgca
     2161 cagtccagcc gcaacgcgta gtcgccgaag ggcgcattca catagtcgca gtgatgaggc
     2221 tgacctgcat cgtcgtactg atgcggacgg aaaaaccgac atgacacgca catccgtgcc
     2281 acagagattt ctccttgttg ttgcaatgtc cggatgactt tcatgaggcc ttttaaaaag
     2341 gccacctgct ccgtggacgt taacgcgctg gccgcctcgg caagaagatt ggggattccc
     2401 actgccgggg cacaagcccg ggcgcctctg gccgtcaact caacattgac gatccgacca
     2461 tctctatgcg aacggaagcg cttgaccaat cgcttccgtt cgagggttgc gactgcctcg
     2521 gacgccgtcg gtaagcgcac cactagttct tgagcaatgg ccgagacttg ggcgctgtga
     2581 tcggccttgc ttcggagcaa ggtgagcact tggcgctgca gaggaccaag accggcttgc
     2641 ccttcacgcc gccaagtcct gctttttagc gccaggctga ttttttcgag cccggccgca
     2701 agttgaaccg gtacgggcag ccgatctttc ggcaacggct tcgccggttg gttcagtacg
     2761 ctcagttcag ccatggcagc cccttagaga acctcttagc gagtgtagtg ggtcgaggaa
     2821 actccgtcaa gccccaattg ccacgaggaa atctcccggc gagcgctcag tgagcaacat
     2881 caatgaaatt gcacacagaa ctgaacaata acggccgttt taattgacaa tgaaacgtgc
     2941 agaaacaaac taagcggctt tgagaggatc ggcagattcg ctgaaacagt ccggacagta
     3001 ggtataggtt aggcgggatt tcgtagggtc gacgccattc gccatcctat aaacgtcccg
     3061 agtgacccag cgctcatagg cggtttcccc atcgtcatgg aagagttgac agaggctgca
     3121 gacacggatc aagacagctg gatcagaagc catgcgacat ccttttacag tccagcatat
     3181 aggacagcct gccgttcgat gattgggatt tcccctagga agtggtccga ggcaacctag
     3241 tgacacgcgg gaacgatcct agaacagtgg cgcacttcac tggctgatgc cccgaccccg
     3301 tagcctgttg gcggcagcat aatggcgaaa ggcatcccgt ttccgcccga gcttttcata
     3361 cagctgggcc aagttacagt gcgcctcaaa aaagtccggc tcatgcaaca aggcctcttt
     3421 gtacgccgaa atagccgcac ttttctggtg acgatcttcc aacaacacgc caaggttgaa
     3481 gtgagccaga gccagtttgg gttcatgctc cagggcgcga cgataacagg attcagcatc
     3541 ggccaacaga ccgagattgt ggttcaggag acccagatta atatgcgcgt cggtaaaacc
     3601 agggtgtaac cgaatggctt cctgataggc ctgtcgggcc tcctcgggag aatcctctac
     3661 caaggtcaat gctcgctgaa accacctctc ggccgtttct cgttcattcc gctggacctg
     3721 tggtgctggc cgcagcgagc gaggtgggag catctgtgcg gtttcgaaat tcagaagaaa
     3781 ttggccagaa tccggttgcc agcgcgaggc accatcccat acaaccacgc gtttccccga
     3841 ggcataaatt tttacgctgg cgatggaagt gtcctcctcc agttgccgtt ccaggcattc
     3901 cagaactttt cggatcttgg cgaccgacac gcccgcttcg gtaaggcctt tcgtcgttcg
     3961 cagcacgacg agattctgaa atgagtacgt gcatgaccgg tttacgcctg gtagaggcag
     4021 aagagcggca cggagacatg cccgcaattg ggacccatcg agacggagaa tacggcagag
     4081 gtcggctgcg gagtagtctc ccgtcatcgt ctcccggagg aagccttctt ggctggttca
     4141 cgagcagttt gagccggcag agcgcgcgat cgagcggttt ttttctgcgc agtgttcgcg
     4201 agactttcct taagccgctg catcagatcc acgacgtcag tgacgggcgc ggcagccttg
     4261 ggagacagtg tgatcgtctt ccctttggat ttctgttcaa tgagctcaat gactcgctga
     4321 cgatattcgt cttgataagc cccagcgtcg aatctatcgg tggaaatcga gtcaatcaat
     4381 tgtgtggcga gcttaagctc ggcagaagcg ggcttctggt cactcttcgg cacttctata
     4441 aatggccgta tttcatcatg gtaatacatc ctgtgcagca gtaagcgacc atccactgaa
     4501 cggatgacat gcaggctgtc ctttcctcgc cagacgtatt tcgcgagagc gacccgctgc
     4561 atatcttcca tcgcttgcgc caataattga tatgcctttt ccccctgctt ctccactcct
     4621 agataatagg tgctttcgaa atagatcgga tcgacggcgc ttaacggaac aaattccaag
     4681 atttgcatgg cctcactttc cgccgcctgc aatcggtcca gctcttcctg atcgatacga
     4741 acatattcgt ccttcgtaat ctcataacct tttgtgagtt cctcgtactt cacctcccgt
     4801 tgacagacag gacagaaccg ctgttgcttg atgcggctgc cacagactgc atgcaactga
     4861 ttgaaatgga tgtcttgctc gtcgatggcg gaataaatcc caacgggaat ggtcacaagc
     4921 ccgaacgaaa tgtgtgccgt ttcaagccga cgtcccattg tccgtagagt gcgtcgcctc
     4981 aaatgcgggg ggtactagga gaaaccctag cttatccagc cttgcgtcaa ggtcttgaat
     5041 cgagatcgac gacgggtgct tgcttgtgct gcgtgtgccg actatgacca taggctatgc
     5101 cgactcagcg tagcgcattc aacctttacg ccgcccgtcg gagagtcctc cgagccgttc
     5161 ttcttctcgg ctttttcgcg cgatcgcgct tcgtgccgtt gatcttcctg gccataagag
     5221 cttccttctg ctctctcgct ttctgctcta atctctccca atccaggtca gccttttctg
     5281 ccttcggaaa catcgtacct tcttcttctt tgacgtggtg tttgatgttc tctcctaaga
     5341 ctttaaactt cgcgtggtat cgatcatcgc tggggcgcat cctctttaat tcgttcatta
     5401 atacatgggc gacgtggtgc tcctccaagg cttcatccat gatgtcttct tcatccaatt
     5461 tctttcgaat tgcaggatag atgaggcttt cctctaattt tgcatggact tctaactcaa
     5521 aaagcgtttc ttgaatgatc cgttctctcg aacgggcatc ctgcgcgtct tcgaattgtt
     5581 caaataagtc tttgaccttt gcatggtcct tctccaacat ttcaaacaca ggatttgacg
     5641 atccgaataa agacatagtc cctccttgct atgctgacaa tggacattca ctctgcttaa
     5701 ggaagaccac tagttcaacc atcctatgcg tcaacgaagt cacaccgaac tagtgagccc
     5761 ccttgctaca aagagagagg caccactact ctgacaggca gctagaaaga tgaggccgat
     5821 gatcctcctg ccctacggcg tctttagagg gacaaggaag aggcaaggtg gtttccagaa
     5881 gccctatggc ggacagagtt tagttgccca tggaagcgag ttaatcggtg aattcgatgg
     5941 caaccgtcga agtaaggaac cttcgtgaga gagaaggttg gaagttttct atctgcacga
     6001 cactctgctt ccgacctgca tcggcctttc cagagaacaa aggtgagtgc ctctggcccc
     6061 tcgaaacgtt ccttctggtg tggaggcgcc gagcggaatt gaaccgctga atcacagcgt
     6121 tgcaagctga gcccttggcc acttaggtac ggcgccgggg tcagaataag aggccgccca
     6181 tcgctgccgg tactagttgt tacccgagct actagtaaaa ttaccatttc gagaggaaaa
     6241 agcagagctg cggacgagca gaagtttatc ctctcaattt cattttacct cgagagaatt
     6301 taacgtgaaa ttaacaggtt gatgaacact ttgtcatttg tagaagaata gaataccgcg
     6361 gactctacag aatgttatga cgaggtgtac aaatggaatt aagaatggac gttcatcagt
     6421 ccttgctacg cacgcatggt ctctcgcatg aatccgccac cataattctc tcgtctcgga
     6481 tggaggtctt atatgcctct cagcaagcgc aagacattct caaagaacta aaaggaatca
     6541 ttcttcgccc ggatgtctcg attcctcctg ccatactcgc ctatattggg cagcgggtga
     6601 tgaaacagat tcagatgcag cgcatccaag ccaattttgc tcctattttt gaccaatgct
     6661 caatcaaagc aagacacgga tcctatcaat gctcatcact cggtattcct tcaaaaactg
     6721 cgattgatca cgctcaaata attctcgttc tgcgtgaaga tcgttggacc agtcttcgtc
     6781 ttcgacccgt aaaccccaca cggcgatccc tgtgggcaca tttctcacga ttgtgttcct
     6841 taaccagagt tccctcccct gcgattcagc aaaggaacta gacgagcttt cccttcccgt
     6901 ttagtttggt ccgcccggtt aactgcttgg actagatgcg accaaaaagg tagctcgcag
     6961 gcaatgagtc cctgagatag taaagcttcc cgaaatgcaa aggggccagt ctttcgaccg
     7021 accccttcaa tcctgagtat tatgttcggc tcaaatcagc gagtcagagt cgccgcttcg
     7081 attgttccct caagggttcg ttctcaagca acttcagtca ttgtctgact gattgggtgt
     7141 ctgcttgagg tggtgcctct cccaagattt cgactacgtc agacgtcaac gtaatggttg
     7201 g
//
LOCUS       DASTQY010000194        13799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1197372, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000194 DASTQY010000000
VERSION     DASTQY010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 13799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13799
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197372"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFR82_06455"
     CDS             complement(<1..551)
                     /locus_tag="VFR82_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501275.1"
                     /translation="MISSRRVLILIFLLGVTFLIAHTINAVIAEALYVPSGVALPRAV
                     DQDSAASRSPAALAEQVRMSGLFPLPADPIAAMGGTITGGPTGVPMRQTLNLASKIKV
                     LGVVVGDRDGISAIIEELGSKRQLFLRLHDEIPDIGELSEIRRNGIVVRQGNQQEFLE
                     LSTGQEKPPLPQPVSTGPATSGGG"
     gene            755..1765
                     /gene="gmd"
                     /locus_tag="VFR82_06460"
     CDS             755..1765
                     /gene="gmd"
                     /locus_tag="VFR82_06460"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEU4501276.1"
                     /translation="MLVKKALLTGITGQDGSYLSEFLLKKGYEVYGIIRRSSSFNTGR
                     IDPIYEDPHVPQRRLHLVYGDLNDASSLNRILRTVQPHEIYNLGAQSHVRVSFDIPEY
                     TGEVTGLGTIRLLEAIRESGLKPKFYQASSSEMFGKVLEVPQRETTPFYPRSPYGAAK
                     VYSYWITVNYREAYNLFACNGILFNHESPRRGETFVTRKITKAAARIKLGVQQDLYLG
                     NLDAKRDWGYAGDYIEAMWLMLQAERPDDYVIATGETHSVREMLELAFDRLQLDWKKH
                     VKIDEKYYRPTEVDLLIGDASKAKRQLGWAPKVGFHELITRMVDADLAAERERLDGIR
                     GK"
     gene            1768..2730
                     /locus_tag="VFR82_06465"
     CDS             1768..2730
                     /locus_tag="VFR82_06465"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEU4501277.1"
                     /translation="MAEFWSDKRVVVTGGAGFLGSFVVEQLRTKGCRAVVVPSSKDYD
                     LVEMDAVRRLYADANPDVVVHLAARVGGIGANQANPGRFFYDNLMMGSQLIEVGRQLG
                     IKKFVAIGTICAYPKYAPIPFKEDDLWAGYPEETNAPYGLAKKMMLVQSQAYRQQYGF
                     NSIVLFPVNLYGPRDNFDLETSHVIPALIRKCAEAQQNGKPEIVLWGDGTPTREFLYV
                     EDAAEGILLAAEQYNESRPLNLGTGEEISIKNLAGMIAAELGFHGRILWDHTKPNGQP
                     RRCLDVSRIKQTIGFQAAHSLRDGLKKTIQWYRANRDALREVRF"
     gene            2923..3387
                     /locus_tag="VFR82_06470"
     CDS             2923..3387
                     /locus_tag="VFR82_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501278.1"
                     /translation="METRVDAAEKYERGVTLRKAGLFKQAIQEFEQAAEDRTYALKAH
                     AQIGLCHKSLGRHEEAVVSFRNALKSPQSSTKETVQILYVLGRSLELLGRTGETLEVY
                     RWIRREDPAYRDVESRIQQLSARRTSIRSRQSSASWIGGILRSCQDLLRMPR"
     gene            complement(3391..7083)
                     /locus_tag="VFR82_06475"
     CDS             complement(3391..7083)
                     /locus_tag="VFR82_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU4501279.1"
                     /translation="MSTSTPQRKPNRLVHETSPYLLQHAYNPVDWYPWGTEALDTARQ
                     RNCPILLSIGYSACHWCHVMERESFENESIAAVMNRCFVCVKVDREERPDLDEIYMAA
                     TVAMNHGQGGWPMTVFLTPEQQPFFAGTYFPPAGRWGRPGFPDLLQQIADVWNKDPNG
                     VRSQAQDMTARLRREWQTPAPISVGEEVLDVVVEEFKQEFDARFGGFGGAPKFPPAAA
                     LSLLLRCYRRSGNPMALQMVRTTLDAMAAGGIYDHIGGGFARYSTDERWLVPHFEKML
                     YDNALLGKVYLEAFQVTANESYRQVVTETLDYVLHEMTDAQGAFYSATDADSEGEEGK
                     FFVWTPEQVAEALDNEEDRRRFCAVYDITKQGNWEHKNIPNRMRPLDVIAQELHVPIE
                     ELRNTLQRGRALLYEARTRRVPPGLDDKIITAWNGMMISAMAEAGRVLGRAEYMEAAR
                     RSAEYLLRMHYTKDNRLLRTSRKGRAQHNAVLEDYAYLGEGLVDLYEASGEERFLERA
                     GALAVSLLEAFQDHDLGGFYTTAKDHETLIVRGREGADGATPSGNAVAATLLARLSFH
                     LDRPEWREAAVAAMRAYGKQMTRYPRGFAKSLSLVDFLTEGPVELALIGAEDDPAFSA
                     LHDTVRSVYLPNRVLATSHRTDGASSHPLLAGKKPVDGRTALYICRDFSCQEPLVDPV
                     SAAEALQRLSTRSTSGVGGKKLRGRILGGSATVEGTARYAARIVNQPRTGGRFAHGYT
                     PFGSTGLTASRLGFGGYRVDENDPEHRRALMHALETGVNVIDTSTNYMDGGSERMIGT
                     VLRELVNKRAVSRDELIVVTKIGYVQGQNLEEAEARERTGRPYPDMVKYGDGIWHCIH
                     PEFLADQLDGSLDRLGLATVDVCLLHNPEYALSEAARSGAVSLVESRARFYHRIQQAF
                     AYFESQVAAGRIKWYGVSSNTIGRDKEDPEAVSLFELSSAASSAAASVGMTRHHFAVL
                     QCPMNLFESDVATRPNTGPQHGQSVLQFAQQGQIAVLVNRPLNAMPGTGAGMVRLAEF
                     QLEGESGNLEETFQAVDELEDEYRSTLAAVIPHGGKGLEPKDYFNWAKELAPLRTRLQ
                     GLEHWEHVEHQMIAPHVNQVLQTIPRVLRGTSAEQQWESWRDRYVPRLLALLGLLRRT
                     AAERSRARTQQIAASIDPLLPESKRMDSLSRKALWVIASTPGVTCVLNGMRTAQYVED
                     STAILGWNRLSDPEAVYKAIANLSAL"
     gene            complement(7209..7436)
                     /locus_tag="VFR82_06480"
     CDS             complement(7209..7436)
                     /locus_tag="VFR82_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501280.1"
                     /translation="MKRALMTIMAVAVAVAFSAPSFAGEENKDKKDEKKGGHVLVYGD
                     ENKDKKDEKKGGQMDTFGEKKEEKKDEKKGK"
     gene            7610..9052
                     /locus_tag="VFR82_06485"
     CDS             7610..9052
                     /locus_tag="VFR82_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEU4501281.1"
                     /translation="MNDIGPYSNYRLTVRLELANKPGIFAKVASALAEEGANLGAVDL
                     ASATADRMVRDVTFDVRDEAHGERVLAKLNDIPDVTVLSASDRIFLLHLGGKIQVKSK
                     LPLTTRNVLSMVYTPGVGRVAQAIAKDKSKAYAFTTKSNTVAVVTDGSAVLGLGNLGP
                     EAALPVMEGKAMLFQELAGIDAWPICLATQDPDEIVRSIQAIAPGFGAINLEDISAPR
                     CFEIERRLKASLDIPIMHDDQHGTAVVLLAALTNALTVVGKHIQQVRVVVNGLGAAGT
                     ACCRILLAAGVSHLLGCDKEGIVLYGDAAQLRACRTDLTACLTPERPHGSLRDALRNA
                     DVFIGLSVGNLLDAEDLDLMAPDRIVFAMANPDPEVPPQLALSHCRIFATGRSDFPNQ
                     INNALAFPGIFRGALDVQASEINEPMKLAAARALAETIPRAALSDDYIVPSLFDKDVV
                     PRVARAVAAAARQSGAARRRSKPFDERPLI"
     gene            9162..9794
                     /locus_tag="VFR82_06490"
     CDS             9162..9794
                     /locus_tag="VFR82_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEU4501282.1"
                     /translation="MPFSAAKYMKFRNGMNKRIGSLRSKTEDSMEIAVDTMMEERVDS
                     YFRIEQGLDEIIKSLLQIEEELEQICDLSGAMRLESRLEFVEDRWDDLDSEIRERPRR
                     RRRRISLADMLKAAGGGGGDLSQSSGSVNNAVDAYAIMGVEFGSSLAEVTAAFRQKAK
                     LLHPDANHGDRSSEPELRRMLDAYQFLKEYLSLSNTEPMQPPKRPYSPAE"
     gene            9802..11016
                     /gene="rnd"
                     /locus_tag="VFR82_06495"
     CDS             9802..11016
                     /gene="rnd"
                     /locus_tag="VFR82_06495"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEU4501283.1"
                     /translation="MTTPPEERYITDSAALEALCKQLRSSRRVGLDTEFVGEDSFIPK
                     LELIQVATHDLAAVIDFPAVQAKGSLAPLWDVICDPHIEKIVHAGRQDLDLFAIHAGQ
                     IPQPFFDTQIAAAMLGFGAQVAYANLVQRIHGTKLAKAHTFTNWSARPLSSDQIAYAA
                     EDVQFLLPIHTYLQRRLQALGRTEWVREEFSRLETSVGEKNKDPLDRYQRIRGWDTLK
                     PRHAAILRDLAAWREAEARRRNVPRGRVIRDEVLLQLARHPPRSVDELRGLRGLHGSE
                     IERNGEQLITLITTALELPPSAWPEVPRERKPEPESVGLVELLQAVLKARSAEMEIAP
                     TLLATTADLQALVEAKEKKRQADLPLLRGWRRQLIGDLLTQILDGSVTVSVDPAKGVL
                     RMSGDTVTTSLS"
     gene            11217..12515
                     /locus_tag="VFR82_06500"
     CDS             11217..12515
                     /locus_tag="VFR82_06500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU4501284.1"
                     /translation="MLLIVHNILKAILAFVVPGGLLFLLALGFLRPSGLPLWCQSPVA
                     ALPYLALVFGLVFGWYFVSARMLFTLVVLTFADRALLAFPFQKDPDPVNQIVFSTSAF
                     LVPMNILGFSILREGRAGTIWHAMAAMLLLVQPLVVLWLCQPGQQDLALALVTSGLTR
                     WPSGWTPIPQAALLAFFIAVTLQMSRFALRRNPMDAGAAWALGTVLVAYHGMHFGWNP
                     THYFSVAALIVFVSLVQASYQETYRDELTGISGRLAYEESTAQLGSNFALAVLAIDQL
                     KSYAGSHGRPVVEQVLKLMSQKVESACHGGRVFRVSGEELTLLFPHRSAMEALVALNE
                     VRKTVESASLFLRGRDHVWDNTGGTKSPGPRDRALPVTVSIGVAEKCGDAASLELIVK
                     SAYRALYEAKAGGGNAVKRGAVTRQSLHRSSGKIVTASEY"
     gene            complement(12520..12870)
                     /gene="arsC"
                     /locus_tag="VFR82_06505"
     CDS             complement(12520..12870)
                     /gene="arsC"
                     /locus_tag="VFR82_06505"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248978.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEU4501285.1"
                     /translation="MSKITIYHKPTCSTCRQAVQLLKESGKPFTAINYYEQRFTASQL
                     KMLLKKANLSARDVLRTKEDAYKQLGLARTSLTDDELIEVMVRHPDLIQRPLVEQGEK
                     ALLARPAEKVSDLL"
     gene            12918..13319
                     /locus_tag="VFR82_06510"
     CDS             12918..13319
                     /locus_tag="VFR82_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501286.1"
                     /translation="MLLSTPYLFLTMPTVGTSSSGQFSCATPTQHTLRDLRTKRKGQP
                     VFVMGHVLERKGQEATFEVFNDRLAVVKFIDGVAIGYDPYELLLPTDIDPDGTAYFEI
                     RSCAQCQQLFPLTTDECDSDPEPTACPECRG"
     gene            complement(13338..>13799)
                     /locus_tag="VFR82_06515"
     CDS             complement(13338..>13799)
                     /locus_tag="VFR82_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501287.1"
                     /translation="LARRVNASPQLVYALGLTLGQTARTGMKCYWALAVAVSILIGAG
                     RLGYADDELLIFAVVSEVPRDKSRVPVRASVNDVAVDTKLLASDAILNNLVWKKLEIC
                     HAMRVEGTKAPDGYRVVTVRIIDAGMLPMSLQSFAGDCLIKKAIEVAPLVD"
BASE COUNT         2959 a         4058 c         3862 g         2920 t
ORIGIN      
        1 cctccgccag acgtggccgg tccggtcgac acgggctgag gcagtggagg tttctcttgt
       61 ccagtcgaca gttccaggaa ttcctgctga ttgccttgcc ggaccacgat gccgtttcgc
      121 cggatctcac tcaactctcc gatgtcggga atctcgtcat ggagccggag aaacagctgg
      181 cgcttgctgc caagctcttc gatgatcgcc gatatgccat cacggtctcc aaccactacg
      241 ccaagcacct tgatcttgga cgcaaggttt aaggtctggc gcataggtac gccggttggt
      301 ccgcctgtta tggtccctcc catcgcggca atcgggtctg ccggaagtgg aaacaagcca
      361 ctcatgcgca cctgttcggc gagcgcagcg ggggaacgag aggcggcact gtcctgatcg
      421 acggctcgcg gcagtgccac gccggaggga acatacagtg cctccgcaat gacggcgttg
      481 atcgtatgag cgatcaagaa tgtcacgccg aggagaaaga tcaatatgag cactcgacga
      541 gaggagatca taggagaggg cagagctccg caaacattgt cggttattgt aatgtgcctc
      601 gctaggaggc gcaatagata gcggcttttg gtcacggttg cgctaagagc gtcagtagga
      661 atgccctgct cccggcatct gtcgcgaccg catgagatgg ctgactcagt ggacaatcga
      721 tacggttcga tccataattt ctcaaccaaa ggagatgctc gtgaaaaaag cgctgcttac
      781 cggcataacc ggtcaggacg ggtcatacct ttctgaattt ctcctgaaga aggggtatga
      841 agtgtacggc atcatccgcc gctcgagttc gttcaatacc ggccgtatcg atcccatcta
      901 tgaagaccca cacgttccgc agcgccgcct gcacttagtg tacggcgacc tgaacgatgc
      961 cagttcgctc aatcgtattc tgcgaaccgt gcaaccgcac gaaatctaca acctcggcgc
     1021 gcagagtcac gttcgagtca gcttcgacat tccggaatat acgggagagg ttaccggcct
     1081 gggcacgatc cgcctcctgg aagccattcg agaatccggc ctcaaaccca agttctatca
     1141 agcgtcctcc agcgaaatgt tcgggaaagt actcgaggtg ccccaacgcg agaccacgcc
     1201 tttttacccg cgcagtcctt acggcgctgc gaaggtgtac tcctactgga tcacggtcaa
     1261 ctaccgcgaa gcctacaatc tgttcgcgtg caacggcatt ttgttcaacc atgaatcgcc
     1321 acgccggggc gaaaccttcg tcaccagaaa aattaccaag gcggccgcac gcatcaagct
     1381 gggcgtgcag caggacctct acctcggcaa tctcgatgcg aagcgggact ggggatacgc
     1441 cggcgactac atcgaggcga tgtggctgat gctgcaagct gaacgccccg acgactacgt
     1501 catcgcaacc ggtgagacgc atagcgtacg cgaaatgctt gaactcgcat ttgaccggct
     1561 ccagctggat tggaagaaac acgtcaagat cgacgagaag tactatcgtc ctaccgaagt
     1621 ggatctgctg atcggggatg ccagcaaagc taagcggcaa ctcggatggg ctccaaaggt
     1681 cggcttccat gaattgatca cgaggatggt cgacgcagac cttgccgccg aacgggaacg
     1741 gctggacgga ataagaggaa agtaacggtg gcggaattct ggtccgacaa gcgcgtcgta
     1801 gtgaccggcg gggctgggtt cttgggctcg tttgtcgtcg agcagctccg gacaaaaggc
     1861 tgccgcgccg tcgtggtccc gagcagcaag gactatgatc tcgtggagat ggacgccgtc
     1921 cgtcgtctct atgcggatgc gaatcccgat gtggtggtcc atctggctgc ccgggtcggc
     1981 ggaatcggag cgaatcaggc gaatcctgga cggtttttct atgacaatct catgatgggg
     2041 agtcagctca ttgaagtcgg ccgtcagctg ggcattaaga aatttgttgc catcggaacg
     2101 atctgcgcct acccgaaata tgctccaatt ccgttcaagg aagacgatct gtgggccgga
     2161 tatccggagg aaaccaacgc gccctacggc cttgccaaaa aaatgatgct cgtccaatca
     2221 caagcttatc ggcagcagta cggattcaac tcaatcgtgc tgtttccggt caatctgtac
     2281 gggcctcgtg ataactttga tctcgaaact tcacacgtga ttccagcact cattagaaaa
     2341 tgcgcagagg ctcaacagaa tgggaaaccc gagatcgtgc tttggggcga cggtaccccg
     2401 accagagagt tcctctatgt cgaagacgcg gcggagggca ttctcttggc ggccgagcaa
     2461 tacaatgagt cacgtcccct gaatcttgga acaggggagg aaatctcaat caagaatctc
     2521 gccggcatga tcgcggctga actcggcttt cacggacgga tcctgtggga ccacaccaag
     2581 cccaacggac agccccgccg ctgcttggat gtcagccgca tcaagcagac catcggcttt
     2641 caagccgcgc attcacttcg tgatggcctg aagaagacga tccagtggta tcgagccaac
     2701 cgcgatgcgt tacgagaagt ccgcttctaa ttctaaggct gtacgactcg ggcatttatg
     2761 cgcgtcggat tacagaaagt ggcgcgacga ccgtccatcc tttttggctg cttactaacc
     2821 ctttgaccca taaagacaga ttatagggtc tttgtggtcc ggcttttgct caaatccgga
     2881 atggaaggca caactttcac caccagcctt gtgaaaggtt ccatggaaac cagggtagat
     2941 gccgctgaaa aatatgaacg gggagtgacg ctgagaaaag cagggttgtt caaacaggcc
     3001 atccaagaat ttgaacaggc ggcggaagac cgaacctatg cgctcaaagc acacgcccag
     3061 atcggcctgt gccacaaatc actgggacga cacgaagaag cggtggtgtc ttttcgcaat
     3121 gcgctgaaat cgcctcagtc atcaacgaag gaaaccgtcc agattcttta tgtcctcggc
     3181 cgctccctgg aattgttggg gcgcactggt gaaaccctcg aagtgtatcg gtggatcaga
     3241 cgcgaggatc cggcttaccg ggatgtagaa tccaggatcc aacagttgag cgcgagacgg
     3301 acctccatca ggagcagaca gtcgtctgcc tcctggatcg gagggattct caggtcatgt
     3361 caagatttgt tgcgcatgcc aagatagccg ttacaacgcc gagagattgg caatcgcctt
     3421 gtacactgcc tccgggtcgc tcaaccgatt ccatcccaat atcgcggtcg agtcttcaac
     3481 atactgggca gtcctcatcc cgttgagcac gcaggtaacg ccaggcgtgc tcgcaatcac
     3541 ccacaaggcc tttcgtgata gagagtccat tcgtttcgat tcaggaagaa gcggatcaat
     3601 cgaagcggct atctgttgcg tccgggcgcg acttctctcg gccgccgttc gacgcagcaa
     3661 tcccaggagc gcaaggagtc gtggaacgta ccggtctctc caactctccc actgttgctc
     3721 ggcagacgtc ccgcgaagca cccgaggaat cgtctgcaag acttggttca cgtgcggcgc
     3781 gatcatctga tgctcgacgt gttcccagtg ctccaatccc tgcaaccggg tgcgtagcgg
     3841 cgccagctct ttggcccagt taaaatagtc cttgggctcc aggccttttc cgccgtgcgg
     3901 aatcaccgca gcgagcgttg accgatactc gtcctctaac tcgtcgaccg cttggaatgt
     3961 ctcctcaagg tttcctgatt caccttccaa ctgaaactcc gccaagcgca ccatgcccgc
     4021 gccggtgccc ggcatggcat tgagggggcg attcaccagc accgcgatct gtccttgctg
     4081 agcaaattgc aagacgctct gaccatgctg aggccccgta ttgggccgtg tggcaacatc
     4141 gctctcgaat aggttcatgg gacattgcaa aacggcaaag tgatgtcgcg tcattcccac
     4201 tgaggcggcc gcgctcgacg ccgcagacga caactcaaac agggagacgg cttcaggatc
     4261 ctccttgtca cgaccgatcg tgttcgaaga gacgccatac cacttaatgc ggccggccgc
     4321 cacttgcgat tcgaaatagg caaacgcctg ttgaatccgg tggtaaaagc gcgcacggga
     4381 ctccaccagg ctcaccgcgc cgcttcgggc cgcttccgag agagcgtatt ccggattatg
     4441 caaaaggcaa acatccactg tcgccaagcc caaacggtcc agcgatccat cgagttggtc
     4501 tgcgagaaac tccgggtgaa tgcaatgcca aattccatcc ccatacttga ccatgtcagg
     4561 gtagggacgc cccgtccgtt cccgcgcctc tgcctcctcg agattctggc cctggacata
     4621 gccgattttg gtcacgacga ttagttcatc tctggaaacg gcgcgcttat tcaccagctc
     4681 acgtagaacc gttccgatca tgcgctcgct tccgccatcc atgtagttgg tagaggtgtc
     4741 gatcacattc acgccggttt ccagtgcatg catcagcgcc cggcgatgtt ctgggtcgtt
     4801 ctcgtcgaca cggtaacctc cgaagcccaa tctcgaagcg gtgaggccgg tcgatccgaa
     4861 tggcgtatag ccatgggcga accgaccacc tgtccgaggc tgattcacga ttcgggcggc
     4921 gtatcgtgcc gtaccttcga cggtggctga accaccgagg attcggcccc gtaatttctt
     4981 gccgcccaca ccggatgtcg accgggtcga gaggcgctga agagcttcgg ccgccgacac
     5041 gggatcgacc agcggctctt ggcaggaaaa atcgcggcag atgtacagtg cagtccggcc
     5101 atcgacgggc ttctttccag caagcaaggg gtgtgatgag gctccatcag tgcgatggct
     5161 ggtggcgagg acgcggttcg gcagatacac gctccgtacg gtgtcgtgta aagcagaaaa
     5221 cgccggatcg tcttcggcac cgatcaatgc cagttcgacc ggcccttcag tcagaaaatc
     5281 aaccaaagac aagctcttgg caaatccccg ggggtatcgc gtcatctgct tgccatacgc
     5341 acgcatggcc gcgacggcgg cctcgcgcca ctcgggacgg tccaggtgaa atgacagacg
     5401 ggctaaaagc gttgcggcca cggcattgcc actcggcgtc gcgccatcag caccttctcg
     5461 accgcgcaca atcagtgtct cgtgatcctt ggccgtggtg taaaaccctc cgaggtcatg
     5521 atcttgaaac gcctccagga gggacaccgc aagcgcgccc gctctctcga ggaaccgctc
     5581 ttcgccgctg gcttcataga gatcgaccaa cccttctcca agataggcat agtcttctaa
     5641 gactgcgttg tgttgtgcac ggccctttcg tgaggttcga agcaggcgat tgtccttcgt
     5701 gtaatgcatc cggaggagat actcggcaga ccgtcgggcc gcctccatgt actcagctcg
     5761 accgagcaca cgtccggctt ccgccatggc agagatcatc atcccgttcc aggcggtgat
     5821 gatcttgtcg tccagacccg gcggcacccg acgggtgcgt gcctcataga gcagcgccct
     5881 gccgcgttgc agagtgttac gcagttcctc gatcgggacg tgtaactctt gtgcaatgac
     5941 gtcgagcggt cgcatccggt tggggatgtt cttatgctcc caattgcctt gtttcgtaat
     6001 atcgtacacg gcgcagaagc ggcggcggtc ttcttcgttg tcgagcgctt cggcgacctg
     6061 ctcgggcgtc cacacgaaga actttccttc ctcaccttcc gaatctgcat cggtggcgga
     6121 atagaacgct ccttgagcat ccgtcatttc gtgaagcacg taatccagtg tttcggtgac
     6181 cacctggcgg taagactcat tcgcggtaac ctgaaacgcc tcgagataga ccttccccag
     6241 caaagcgttg tcgtaaagca ttttctcgaa atgagggaca agccagcgtt cgtcggtcga
     6301 ataccgtgca aacccgcctc cgatatggtc atagattcct cctgcagcca tggcgtcaag
     6361 ggtggtcctg accatctgca gcgccatagg attgccgctc ctccggtagc atcgaagcag
     6421 gagagagagg gcggcagccg gggggaattt cggagcacct ccgaatccac cgaagcgcgc
     6481 atcaaactcc tgcttgaatt cttccacgac cacatcaagg acttcctcac cgacggagat
     6541 cggagccggg gtttgccact cacgccgtaa tcgggcagtc atgtcctggg cctgagatcg
     6601 gactccgttc ggatctttgt tccagacgtc cgcgatctgt tgcaacagat ccggaaagcc
     6661 cggacgcccc caccgaccag cgggcgggaa ataggttcct gcaaagaacg gctgctgttc
     6721 aggagtcaga aagacggtca tcggccagcc accctggcca tgattcatcg cgaccgtagc
     6781 ggccatataa atctcatcga gatctggtcg ctcctcacga tcgactttga cgcagacaaa
     6841 gcaccgattc atcacagctg cgatcgattc gttttcaaac gactcacgtt ccatgacatg
     6901 gcaccagtga caagcggaat agccaatgga cagtaggatc ggacaattcc tctgtcgtgc
     6961 ggtgtctaag gcttccgttc cccacgggta ccagtcaacc ggattataag catgctgaag
     7021 gagataggga ctcgtctcgt gaaccagtcg attgggcttg cgttgaggcg ttgaagtaga
     7081 cataggtcac ggtagcaccg gcttctgaaa gcggtcaatg cgtcaaaaaa aaggtctatg
     7141 gccgtaagac catagacctt gcgggacact caacaggacc agacagtcgg ggaggagccg
     7201 cgactccttt acttgccctt cttctcgtcc ttcttctctt cctttttctc tccaaaggtg
     7261 tccatctggc cacccttctt ctcatccttc ttgtccttat tctcatcgcc gtacacgagg
     7321 acgtggccac ccttcttctc gtccttcttg tccttattct cctcgcccgc gaacgaagga
     7381 gcgctgaaag caaccgccac cgccactgcc atgatagtca ttaacgcgcg cttcataggt
     7441 atcccccctt agactgttcg ttggcctgcc catcaggcaa acgggcgcct accgtagcca
     7501 atgggtcagg gatttgtcaa tgaccacgat acccagacca ttggaatcaa tcgccggtcg
     7561 ctctcctctg tggtaaagtt aagagacagt atttcggccc ctcgctccca tgaatgacat
     7621 cggcccatac tcaaactatc gcctcaccgt tcgactggaa cttgctaaca aaccaggaat
     7681 atttgccaag gttgcctcgg ctctcgcaga agaaggcgcc aacctcggtg ctgtggatct
     7741 ggcgtcggcg accgcggacc ggatggtcag ggatgtgacc tttgatgtcc gggacgaagc
     7801 ccatggcgaa cgcgttctgg ctaaattgaa cgacattcca gacgtgaccg tgctctcggc
     7861 ctctgatcgt atttttctcc tccacctggg agggaagatc caggtgaaga gcaagctgcc
     7921 actgactacc cgcaacgtac tgtcgatggt ctatacgcct ggcgtgggcc gtgtagctca
     7981 agcgatcgcc aaagacaaat ccaaagccta tgccttcacg acgaagagta acactgtcgc
     8041 ggtggtgaca gacggatcag ccgtgttagg cctgggaaat ctcgggccgg aagcggccct
     8101 tccggtgatg gaaggcaaag cgatgctctt ccaggaattg gccggaatcg atgcctggcc
     8161 catttgcctg gcgactcagg atccggatga gattgtgcgc tccatccaag cgattgcccc
     8221 aggattcggg gcaatcaacc tcgaggacat cagcgcacct cgctgctttg agatcgaacg
     8281 tcgactcaag gcgtcgctgg acattccgat catgcatgac gaccagcatg gtaccgcagt
     8341 ggtcttgctc gccgcattga ccaatgcctt gaccgtagta ggtaagcata tccaacaggt
     8401 tcgtgtcgtg gtcaatgggc ttggcgctgc gggcactgcc tgctgccgga tcctccttgc
     8461 agctggcgtg tcccatctcc tcggttgcga taaggaaggg atcgttctgt acggagacgc
     8521 cgcccaactg cgggcctgcc gtaccgatct gacggcgtgc ctgacgcccg agcggccaca
     8581 cggcagcttg cgcgacgctt tgcgcaatgc cgatgtgttc atcggactgt ccgtgggcaa
     8641 cctgttggac gcggaggacc ttgacctgat ggcgccggat agaattgtct tcgccatggc
     8701 caatcccgac cccgaggtgc cgcctcagtt ggcgctttct cactgccgca tcttcgcgac
     8761 cggccgctcg gattttccga accagatcaa caacgcgttg gcgttccccg gtattttccg
     8821 tggcgcgctc gatgtgcagg ccagcgagat caacgaaccg atgaaacttg cggcagcccg
     8881 ggctctggcc gaaacgattc cgagggccgc cctcagcgat gactacatcg tgcccagcct
     8941 gttcgacaaa gatgtcgttc cccgggtggc acgagcagtc gctgcagctg cgcgtcagag
     9001 tggtgcagcg cgtcgaagat cgaagccttt cgacgaacgg cccctgattt gatcccgact
     9061 gagcaagcgc cgtgtttctc ctgagaaatg cacgtgtcct cccttcagcg ccactgtcct
     9121 cctgtgctac aatgcccgga attccctgga gcgtctgcgc catgcccttt tccgccgcca
     9181 aatacatgaa gtttcgaaac ggcatgaaca agcggatcgg ctccctgcgt tcaaagacgg
     9241 aagacagcat ggaaattgcc gtagacacca tgatggaaga gcgggtggac agctacttcc
     9301 ggatcgagca gggcctggat gagatcatca agtctttgct gcagatagaa gaagagctgg
     9361 aacagatctg cgatctatct ggcgccatgc gcctcgaatc aaggctggag ttcgtggaag
     9421 accgttggga cgatctcgac agcgaaattc gcgaacggcc caggcgccgc aggcggcgca
     9481 taagcctcgc tgatatgctg aaggcggccg gcggaggcgg cggtgatctc tcccaaagct
     9541 caggaagtgt caataacgcc gtagacgcct atgccattat gggagtagag ttcggcagct
     9601 ctttggccga ggtgacagcg gcgttcagac aaaaggcgaa gctgttgcat cccgatgcca
     9661 atcacggcga tcgcagctcc gagccggaac tgcggcggat gttggatgcc tatcagttct
     9721 tgaaggaata cctgagcctc agtaacacgg aaccgatgca gccgccgaag cgcccctaca
     9781 gcccagccga gtaatgccac agtgaccacc cctcctgagg aacggtacat caccgacagc
     9841 gcggcactcg aggctctgtg caagcaatta cggtccagtc gccgggtggg gctggatacg
     9901 gaattcgtcg gcgaagacag cttcatcccg aagctggaac tgatccaagt cgccacgcat
     9961 gaccttgctg ccgtcatcga ttttcccgcc gtccaagcca agggatcgct tgccccgttg
    10021 tgggacgtga tctgcgatcc tcatatcgag aagatcgtgc atgcgggtcg ccaagatctt
    10081 gacctctttg cgattcacgc cgggcagatt ccccagccgt ttttcgacac gcaaatcgct
    10141 gcggccatgc tcggcttcgg agcgcaagtc gcctacgcaa atttggttca gcggattcat
    10201 gggacgaagt tggcaaaggc tcatacgttt accaattgga gtgctcggcc actttcatct
    10261 gatcaaatcg cctacgccgc tgaagatgtg cagtttctgc tgcctattca tacttacctg
    10321 cagaggcggc tacaggcgct cggccggact gaatgggtcc gagaagaatt ttcacgccta
    10381 gagacaagcg taggggagaa gaataaagac ccgctcgatc gatatcaacg catcagggga
    10441 tgggataccc tgaagcccag acacgctgcc atcctacgag acctggccgc ctggcgcgaa
    10501 gccgaagccc gccggcgtaa cgtacccagg gggcgcgtga ttcgtgacga agtgctgtta
    10561 caattggcgc ggcatccgcc gcgcagtgtt gatgaactcc gtgggctccg cggcttgcac
    10621 ggatctgaaa tcgaacggaa cggcgagcag ttgatcaccc tgatcacaac ggcgctggaa
    10681 cttccgcctt ctgcctggcc tgaggtgccc cgtgagcgca aacccgaacc ggagtccgtc
    10741 ggattggtgg aactccttca agcggtattg aaggcacgct cggcagagat ggaaattgct
    10801 cccacattgc tcgcaacgac agccgatctc caggcgctcg ttgaagccaa ggaaaagaag
    10861 agacaggcgg accttccact attgcgagga tggcgacgcc aattaatcgg cgatctactt
    10921 acgcagatcc tcgatggcag cgtgacggtc tccgtagacc cggcaaaggg agtcctgcgg
    10981 atgtccggag acacggtgac caccagtctg tcataagcct gccgcaccct ttctcgttac
    11041 ctccgcactg ttcaaaaact gccttaggcc atccacggcg tgcatcacca tggcgccacc
    11101 ggtccgtgcg ggggtccgca acccagtgat ttcggcgtct tgcgcaatcc tccacacacc
    11161 cggctgcagc ctcatctggt acagaactgg cacctttggg ctcgacccca tctccgatgc
    11221 tgctcatagt acacaacata ctgaaggcca tcctcgcctt tgttgttccg ggggggttgc
    11281 tctttttact cgcgttaggc ttcctccgcc ccagtggact cccattgtgg tgtcagagtc
    11341 cggttgccgc attgccctat ctcgcgctgg tattcggcct tgtgttcggc tggtactttg
    11401 tcagcgcgag aatgctcttc acgctggtcg tcctcacatt cgccgaccgg gcactcctgg
    11461 catttccttt tcaaaaggat cccgacccag tgaatcagat cgtgttttca acatccgcct
    11521 tcttggtgcc gatgaacatc ttgggattct cgatcttgcg agaaggacga gcggggacca
    11581 tctggcacgc gatggccgcg atgctgcttc ttgttcagcc tcttgtcgtc ctgtggctgt
    11641 gccaaccggg acaacaggat ctagccctcg cccttgtcac ctccggactt acacggtggc
    11701 cgagcggttg gacaccgatt ccacaggcgg cgctcctggc gtttttcatt gccgtgacgc
    11761 tgcagatgag ccgctttgcg ttgaggcgga atcctatgga tgcgggagcg gcctgggcat
    11821 tgggtaccgt gttggtcgcg tatcacggaa tgcatttcgg atggaacccg acccattact
    11881 tctcggttgc agcactgatc gtatttgtgt cactcgtgca agcttcctat caggaaacgt
    11941 accgtgacga actgacgggg atctccggac gcctggcgta tgaagagtcc accgcccagc
    12001 ttggcagcaa ctttgccctc gctgtgctgg cgatcgatca actgaagtcc tatgcgggaa
    12061 gccatggaag gccggtcgtg gaacaggttc tgaaactcat gtcccaaaaa gtcgaatctg
    12121 cctgccacgg agggcgggtg ttccgtgtca gcggggagga attaaccttg ctgttccccc
    12181 accggtccgc aatggaagct ctggtcgcac tgaatgaggt gcgcaaaacc gtcgagtccg
    12241 cctctctgtt tcttcgaggc cgcgaccatg tgtgggacaa tactggcggg acaaaatcgc
    12301 cgggaccgag ggatcgagca ctgccggtga ccgtcagtat cggagtggct gaaaagtgcg
    12361 gagacgcggc ttcgctcgag ctaatcgtca agtctgccta ccgcgccctc tatgaggcga
    12421 aggccggagg gggaaatgca gtaaaacgag gggcagtgac acgccaatcc ctccatcgct
    12481 cgtccggtaa gattgtaact gcgagcgaat actaacgcct tagagtagat cgctgacttt
    12541 ttctgcaggt cgggccaaca acgccttttc accttgctcg accagcggac gctgaatcaa
    12601 atccggatgc ctgaccatca cctcgatgag ttcatcatcg gtcagggatg ttcgggcgag
    12661 gccgagctgc ttatacgcgt cttctttggt tctcaagaca tccctcgccg agaggttggc
    12721 tttcttcaac agcatcttga gttgtgaggc ggtgaaccgc tgctcgtagt agttaatcgc
    12781 tgtgaatggt ttgccactct ccttaaggag ttggacggcc tgccgacagg tcgaacaagt
    12841 gggcttgtgg tatatggtaa ttttagacat acgtgctcca cactcgttgc ttgacgcctt
    12901 tttttcgctt gtggtacatg cttctcagca ctccttatct cttccttacc atgccgacgg
    12961 tcggaaccag ctccagcggt cagttctcct gcgcaacccc cacgcagcat acattacgcg
    13021 accttcgcac gaagcggaag ggccagccgg tgttcgtgat gggtcatgtc ctcgagcgga
    13081 aaggacagga agctaccttc gaggttttca atgaccgtct ggcagtcgtg aaattcatcg
    13141 acggggtggc gattgggtac gacccctacg aactcctcct tcccaccgat atcgatccgg
    13201 atggcacggc ctactttgaa atccgttctt gcgctcagtg ccagcaactc ttccctttga
    13261 ctaccgacga gtgcgactcc gaccccgaac cgaccgcttg ccctgaatgc cgcggctaag
    13321 cagtgggcag tgccgtgtca atcaaccaaa ggcgcaactt caatcgcttt cttgatcaag
    13381 cagtcgcctg caaacgattg cagagacatg ggcagcatcc ccgcgtcgat gatgcggacg
    13441 gtgacaaccc ggtaaccgtc gggcgctttg gtgccctcga cgcgcatcgc atgacagatt
    13501 tccaattttt tccacacgag attgttgagg atggcgtctg aggcgagcag cttggtatcc
    13561 acggcaacgt cattcacaga agccctgaca ggcacgcgtg acttatctct cggcacttca
    13621 ctgacgaccg cgaaaatcaa caactcgtcg tcggcatacc caagcctccc tgctccgata
    13681 aggatgctga ctgccacagc caaggcccaa tagcacttca tgcctgtcct cgctgtctgt
    13741 cccagggtca agccgagcgc gtagaccagt tgtggcgatg cgttaacgcg ccgggccag
//
LOCUS       DASTQY010000195         7814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1422528, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000195 DASTQY010000000
VERSION     DASTQY010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422528"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2631
                     /locus_tag="VFR82_06520"
     CDS             <1..2631
                     /locus_tag="VFR82_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU4501288.1"
                     /translation="FGQQNYSMRIILNPLMMARLSLTPTDIATIIREQNRDYPAGLIG
                     REPAMKGTELTIPVITKGRLTDVKDFEELIVRALPDGSLVRLKDVARIELGAQSYALE
                     GRWNSRPTTFIVTFLAPGANALDTVRATRTVMDKLAKDFPPGVSYDIPYDTTRFIEVS
                     IKEVVKTLVEAMALVILVVYLFLQSWRTTIIPTVAVPVSLIGTFAGLYALGFSINTIT
                     LFGMVLAIGIVVDDAIVVVENVERHMREDRVSAKEAAKRAMSEVTAPVIAIVLVLVSV
                     FVPVGFIGGITGALYKQFAATIAISVTVSGFVALTLSPALCSMFLVHREGERGGFWKW
                     FDRAFGGTQRGYTSIVSKLVARPIRVMLVFGLVVLLSSGMFVSLPRSFLPEEDQGYFI
                     VVAQLPDGASKQRTDAVLERIEKFFQANPAVHSTDAMSGQNFVFGTRGPNAATMFVPL
                     HLWDERTEPQYHVQSLIGMAYAEFAKIPEALLLAFNAPSLRGVGTVGGFSAQLQDPSG
                     GDFKKFAAVAQQFVERAKKEPAIGQVGTNFRVSAPRLYAHVNRERAKALGIPISDAFD
                     TLQAYFGTFYINDFLKFGRIYHVQTEADAEYRSKPQDISKIYIRAQNGQGASMIPLDT
                     VVTTEYQSGPDPVNHFNGYNTALVLGSAARGYSSGQALEALERAANEVLLPQGYDIDW
                     SNISFQERRVGSQSNVVFLIGLLMVFLVLAAQFESWAVPFAVILAVPFAIFGALSAVW
                     LRGFSNDIYFQIGMVTLIGLSAKNAILIVEFANTRYEAGMPLIDAAIEAARLRFRPII
                     MTSMAFIFGMFPLVIATGAGAASRQSIGTGVLGGMLAATFLAIFFVPLFYVLIRKLTG
                     RMAVPSHQVHKATLLPEGEG"
     gene            2633..4048
                     /locus_tag="VFR82_06525"
     CDS             2633..4048
                     /locus_tag="VFR82_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEU4501289.1"
                     /translation="MRPYLLMTIAVLMASCALGPDYERPAADSEDRFRMAEAGGDAPS
                     MANLPWWELLKDEQLQHLVRTALAENRNLQKAAATVEEFQARAWVAKSDFLPNMIASG
                     NAPSFGRKTVFLFPGFPNPFNYYLQANLSWEVDVWGRIRRANEAARADLFAKEESRRA
                     VVLELVSAVAESYFNLLQFDTQLAIAKRTLQSWEESVRIAQARLRQGMTSKLDADQFE
                     AERANAAARIAELERQMIQAENHLSVLLGRKPFEISRGRSLVEQTLPPTIPPGLPSEL
                     LQRRPDILQADRALNAATARIGAAKADRFPKISLTGLLGAADPRTGSIFSDVSSFGAA
                     GAGFTLPLFNAQALGFQQDAVEAQAKESLAEYQQTVLTAFREVEDALVAARTARQQND
                     AQQQQVNALQSALRLAELRYKGGLANYLDVLVARRNLFEAELAMTGSRRLHLVSVVQL
                     YKALGGGWSPEMEQSQEKKDS"
     gene            4054..4656
                     /locus_tag="VFR82_06530"
     CDS             4054..4656
                     /locus_tag="VFR82_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU4501290.1"
                     /translation="MEKAAQAKHRIEDVIAHRWSPRAFHERPVEPEKLKSLFEAARWA
                     PSSNNEQPWRFLAANKEQRSDFDRLFNCLSEGNSKWVFRAPVLILSVASLFFEDDGKP
                     NRHAMHDTGLAVENLVLQATALGLQAHQMAGYDVDKARKECQIPSGFEPVAMIAVGYP
                     GDPAILPEYLRERELKPRERQPIEDFVFSASWGRPSPLLR"
     gene            4752..5630
                     /locus_tag="VFR82_06535"
     CDS             4752..5630
                     /locus_tag="VFR82_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4501291.1"
                     /translation="MADPRQRLDTNVQGNFFVDTTCINCDTCRQLAPDSFQEVGEYSA
                     VTIQPESDERLHQAYQALLACPVGSIGTERSDKTRLQQAMSSFPILLDGGVFYCGFNS
                     EKSFGANSYFIHHSEGNWLVDSPRYIKHLVDAIERMGGLRYIFLTHEDDVADAARYAA
                     RFGATRIIHRADAEAMPDAEWIVDGSDVIPLTPQFDLIPVPGHTPGSSALLYDQRFLF
                     TGDHLWWEPETRSLQAPRRLVWRSRAMVLSLERLLDYRFEWVLPGHGDRIKLTPEDMR
                     NHLRLLVDHRLAALAS"
     gene            5699..7276
                     /locus_tag="VFR82_06540"
     CDS             5699..7276
                     /locus_tag="VFR82_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501292.1"
                     /translation="MRLSYLPPLMVYAAYGISGLTGVVGTFFVKDYLSLSASFLAALG
                     FWAGIPWALKMPIGHLVDLLWRWKSWLVFVGAGLLAVSLGIMAALIGHREAMTTVFSA
                     EVWFVLSALLAPIGYVIQDAVADAMTVEAVPRVNDRGQPFEEQTKKLMHTTMQTLGRV
                     AIIGGGIVVALINVYIFTGAEQLPQSEMVRLYERIYLMALAIPFISVLGIGLAWILQR
                     RHRNRLRREGFTTDQANQMVKVRNDSSAPTHPNWWILGGSVVFVFFTLTVGLGNVPYS
                     EEVVFAGSMAIVLFLMFRLTQELEPDKRFTLVGTAVVVFIFRAIPGPGPGTTWWMIDE
                     LRFDQQFLSVLSLIGSTLTLVGMFVFRRFMAERSIAYVVGFLTIVGTILSLPILGMYY
                     GLHEWTARLTGGVIDARFIALIDTALESPLGQISMIPMLAWIANSAPAHLKATFFAVM
                     ASFTNLALSLSQLGTKYLNEIYVVTREVRDPAAGAIRTPADYSQLGDLLIAQLLLGLA
                     LPLAAIMFARITRFKSA"
     gene            complement(7301..>7814)
                     /locus_tag="VFR82_06545"
     CDS             complement(7301..>7814)
                     /locus_tag="VFR82_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501293.1"
                     /translation="LVLAQLAFVLWLGWFSQWSELLAELPQYILVGLQCFRIPVELLL
                     ATLATRKLLAIEMTYYGRNFDVLEGITAVGLAIWLKRRGENSLRHVVLIWNLMGLSLV
                     TVVLVHGLLSVPYPSQLLHLSIPTFIIAYFPVVWLLTVLVPMAYLLHLVSIRRCLAKA
                     LASSETDQDS"
BASE COUNT         1571 a         2182 c         2300 g         1761 t
ORIGIN      
        1 ttcggccagc agaactacag catgcggatc atcctgaatc cgctgatgat ggcccggttg
       61 agtctgaccc ctacggacat cgccacgatt atccgcgaac agaaccggga ctatccggct
      121 ggactgatcg gccgggaacc ggccatgaag ggcactgagt tgacgatccc cgtcattaca
      181 aaaggccggc tcacggacgt gaaggatttt gaagagttga tcgttcgcgc attgccggac
      241 ggatccctgg tgcgtttgaa ggacgtggct cgaattgaat tgggagcaca atcgtatgcg
      301 ctggaaggcc ggtggaacag ccgccctacg acgttcatcg tgacctttct cgcgccgggt
      361 gcgaatgcgt tagataccgt ccgtgcgact cgtacggtca tggacaagct cgccaaagac
      421 tttcccccgg gcgtgtccta cgacattcct tatgacacaa cccggtttat cgaagtgtcc
      481 atcaaagaag tcgtcaaaac ccttgtggag gccatggctc tggtcatcct ggtcgtctat
      541 ctcttccttc agagttggcg gacaacgatt attccgactg ttgccgtgcc ggtgtccttg
      601 atcgggactt tcgccggtct ctatgcgctg ggcttttcga tcaacaccat cactttgttt
      661 ggaatggtat tggcgatcgg tatcgtggtc gacgacgcca tcgtcgtcgt ggaaaacgtc
      721 gagcggcaca tgcgggagga tcgcgtctcg gccaaagagg ccgccaaacg agccatgtcc
      781 gaagtcaccg cgcccgtgat cgctatcgtg ctggtgttgg tctcggtctt cgtcccggtg
      841 ggcttcattg gcgggattac cggagcgctg tataaacaat tcgccgcgac cattgcgatt
      901 tcggtcacgg tttcagggtt tgtggccctc accctcagcc ctgcgctctg tagcatgttt
      961 ctcgtgcacc gcgaaggaga gcgcggcggg ttttggaaat ggtttgaccg cgccttcggc
     1021 ggtactcagc gaggctatac cagcatcgtg agcaaactcg tcgcgaggcc catccgcgtc
     1081 atgctcgtct tcggtctcgt cgtgttgtta tcgtcaggca tgttcgtcag cctgccgcgc
     1141 agttttctgc ccgaggagga tcaaggctac tttattgtgg tggcgcagct tccggatggt
     1201 gcgtcgaagc agcgcacgga cgcggtgctc gaacggatcg aaaagttttt ccaagccaat
     1261 ccggcggttc attcgaccga tgccatgtcc gggcagaatt ttgtcttcgg cacgagaggt
     1321 ccaaacgctg caacgatgtt tgtgcctctg catctatggg atgaacgcac agaacctcag
     1381 tatcacgttc aatcgctgat tgggatggcg tatgcagaat ttgccaagat acctgaggcg
     1441 ctgcttctgg cctttaatgc cccgtctctt cgcggcgttg gcacagtcgg cggattttca
     1501 gctcagttgc aagatcccag cggcggggac ttcaaaaaat ttgcggctgt ggcccagcaa
     1561 tttgtcgagc gcgcgaaaaa ggaaccggcc atcggccaag tgggtacgaa ctttcgggtg
     1621 tctgcgccgc gtctgtatgc ccatgtcaat cgagaacgag ccaaagccct cggcataccg
     1681 atctccgatg cctttgacac cttacaggcc tacttcggca ccttctacat caatgacttc
     1741 ctcaagttcg gtcgcatcta tcacgtgcag actgaagccg atgccgaata ccggtccaag
     1801 ccgcaggata tttccaagat ctacatccgc gcgcagaatg gacagggcgc cagcatgatc
     1861 cccctggaca cggtcgtcac gacggaatat caaagcgggc ccgatcccgt aaaccacttc
     1921 aacgggtaca acacggcgct ggttcttgga tcggcggcac gaggctatag ttctgggcag
     1981 gcgctggaag ccttggagcg ggccgccaat gaggtattgc tgccgcaagg ctatgacatt
     2041 gattggagca acatttcctt tcaagagcgt cgggtgggaa gccaatccaa cgttgtgttt
     2101 ctgatcggtt tgttgatggt cttcctggtt ctcgcggcgc agttcgaaag ctgggcggtc
     2161 ccgtttgcgg ttattctcgc ggtcccgttt gcaatctttg gcgccctgtc cgccgtatgg
     2221 ttgcgcggtt tctcaaacga catctatttt cagattggca tggtcacgct catcgggctg
     2281 tccgcgaaga acgccattct gatcgtcgaa ttcgccaata ctcggtatga agcgggcatg
     2341 ccactcattg acgcggcgat tgaagcggca aggctgcgtt tccgtccgat catcatgacg
     2401 tcgatggcct tcatcttcgg catgttcccg ttggtcatag cgacgggtgc aggcgccgcc
     2461 agccggcaat ctatcggcac cggggtgttg ggcggcatgc tcgccgccac gtttttggcg
     2521 atttttttcg ttccgttgtt ttacgtgttg atcaggaagc tgactggccg gatggcggtc
     2581 ccctcgcacc aggtgcacaa ggccacgctg cttccggaag gagaaggttg atatgcgtcc
     2641 ctatctgttg atgacgatcg cagtccttat ggcgtcctgt gcgctaggac ccgactatga
     2701 gcggcccgct gccgacagcg aagatcgctt ccggatggcg gaagcgggcg gcgacgcgcc
     2761 ttcaatggcg aacctcccat ggtgggaact tttgaaagac gagcagcttc aacacttggt
     2821 ccgcactgcg ctggctgaaa atagaaatct gcaaaaagcc gcggccaccg tcgaggagtt
     2881 tcaggccagg gcctgggtgg ccaagtcgga ttttcttccc aacatgatcg cctccggcaa
     2941 cgctccgtcg ttcggccgga agaccgtttt cctgtttccg gggttcccga atccattcaa
     3001 ctattacctc caggccaatc tgtcttggga ggtggatgta tggggacgga tccggcgtgc
     3061 caatgaagcg gcgcgcgccg acctcttcgc gaaagaagaa agccggcggg cggtggtatt
     3121 ggaactggtc agcgcggtgg cggaatccta ctttaatctc ttgcagttcg acacgcagct
     3181 ggcgatcgcc aagcgcaccc tgcagtcctg ggaggaatcc gttcgtattg cgcaggcgcg
     3241 attacgccag gggatgacgt caaaactgga tgccgatcaa tttgaggccg agcgagcgaa
     3301 tgccgcggca aggatcgccg aattggagcg gcaaatgatc caggcagaga atcatctgag
     3361 cgtcctgctt ggcagaaaac cctttgagat ttctcgcggg aggtcgctcg tggaacagac
     3421 gctgccaccc acgattccgc cgggcctgcc ttccgaactg ctccagcggc gcccggatat
     3481 cctgcaagcc gatcgtgcgt taaacgccgc gacggcccgg atcggcgccg ctaaggcaga
     3541 ccgctttccg aaaatttcgc tcacgggcct actcggtgcg gccgatccta gaaccggtag
     3601 catcttttca gatgtgtcgt cgttcggcgc ggccggagcc ggcttcacgc taccgttatt
     3661 caacgcgcag gcactgggat tccagcagga cgcggtggag gcccaagcca aggaatcgct
     3721 ggcggagtat caacaaacgg tgctcacggc gtttcgcgaa gtcgaggatg ccttggtggc
     3781 cgccaggact gcccggcagc agaacgacgc gcagcagcag caggtgaatg cgttgcaatc
     3841 tgctctccgt ttggccgaac tccgttataa aggcggactc gcgaactacc tggatgtgct
     3901 ggtcgcgcga cgaaaccttt tcgaggcgga attggccatg accggctctc gccggctgca
     3961 cctggtttcg gtcgtccagt tgtacaaggc gctcggtggc ggatggtcac ctgagatgga
     4021 gcagagccag gaaaagaaag atagctgatc gccatggaaa aggcggctca ggcgaagcac
     4081 cgtattgaag acgtgattgc acatcgctgg agtcctcgtg cgttccacga acgtccggtg
     4141 gagccggaga aattgaaaag cctgttcgag gcggcgcgct gggcgccttc ttcgaataac
     4201 gaacagccgt ggcggtttct ggctgcgaac aaagagcagc gttccgattt cgacagattg
     4261 tttaattgtc tctctgaggg gaattcgaag tgggtatttc gtgcaccggt attgatcctc
     4321 tctgtggcga gcctgttttt tgaagacgac ggaaaaccca accgtcatgc tatgcatgac
     4381 actggcttag ccgtcgagaa cctcgtcctg caagccaccg cccttgggtt gcaggcgcac
     4441 cagatggccg gatacgatgt ggataaggcg cgcaaagaat gtcagatccc gtctggattc
     4501 gagcccgtgg ccatgattgc ggtaggatat ccgggtgatc ccgccattct gccggagtat
     4561 ctgcgcgagc gtgagctgaa gccgcgcgag cggcaaccga ttgaggattt cgtgttctca
     4621 gccagctggg gccggccgtc gcctctcctg cggtgaaccg gtggctcact cgtcccccgc
     4681 catcgcgcat ctttcgggcg gcggaggaga cgaagcggaa gcgatcgatc ccacggatca
     4741 agggttaatc aatggcggat ccaagacagc gtctcgacac aaatgttcag ggcaatttct
     4801 ttgtcgatac gacatgcatc aactgcgata cctgccggca gctggcaccg gacagctttc
     4861 aggaagtggg cgaatattcc gccgtgacga tccagcctga gagcgatgaa cgtctgcatc
     4921 aggcgtatca ggcgttgctg gcctgtccgg tagggtcaat cggcaccgag cgtagcgata
     4981 agacacggtt acaacaggcg atgtccagct ttcccatcct cctcgatgga ggggtgtttt
     5041 actgcgggtt taattctgaa aagtccttcg gtgcgaacag ttatttcatc caccattccg
     5101 agggaaactg gctggtagat tcgcctcgct acattaaaca tctggtcgat gccatcgagc
     5161 gcatgggagg tctcaggtat attttcctta cccatgaaga cgatgttgcc gatgccgccc
     5221 gatacgcagc gcggttcggc gcaacccgca tcatccatcg tgccgacgcc gaagcgatgc
     5281 cggatgcgga atggatcgtg gatggttcag acgtcattcc gttgactccg cagtttgatc
     5341 tcatcccggt gcccggacat acacccggca gtagtgcgtt gctctacgac caacggtttc
     5401 tgttcaccgg cgaccatctc tggtgggagc ccgaaacccg atcgctccaa gcgccacggc
     5461 ggctggtctg gcgatcgcgt gcaatggtgc tgtcccttga aaggctgctc gactatcggt
     5521 tcgaatgggt gttgcccgga cacggcgatc gtatcaagct cactccggag gacatgcgaa
     5581 accaccttcg cttactggtc gatcatcgtc tcgcggccct tgcctcctaa tcccatccct
     5641 gatgctcctg tcgatatacc aatggactga ccgcaacatc ctggcgctag gacgtgagat
     5701 gcgcctgtcc tacctcccgc cgcttatggt ctacgcggcc tacggtattt cgggtttgac
     5761 cggcgttgtc gggacgttct ttgtcaaaga ctatctgagc ctttccgctt cgtttctcgc
     5821 cgcgctcgga ttctgggccg gcattccctg ggcactcaaa atgccgattg gtcatctcgt
     5881 cgatttgtta tggcggtgga aaagttggct cgtgttcgtg ggcgcggggt tgcttgccgt
     5941 cagcctgggg atcatggccg cactgatcgg ccaccgggaa gccatgacga cagtgttttc
     6001 ggccgaagtg tggtttgttc tgagcgctct cctggccccc atcgggtatg tcatccaaga
     6061 tgcggtggcc gacgccatga cggtcgaagc ggttccacga gtgaatgatc gtggtcagcc
     6121 gtttgaggag cagaccaaga agctcatgca taccaccatg cagacactcg ggcgtgttgc
     6181 gatcatcggc gggggcatcg tcgtggcgct gatcaatgtg tacatcttca cgggtgctga
     6241 acagctgccg caaagtgaga tggtccggct ctacgaacga atctatctca tggcgctggc
     6301 gattccattc atctcggtgc tcggcatcgg attggcttgg atactccagc gccgtcatag
     6361 gaatcgactg cgacgcgagg gattcaccac cgatcaggca aatcaaatgg tcaaggtgcg
     6421 caatgattcg agcgcaccga cacatcccaa ctggtggatt ctcggcggga gcgtcgtgtt
     6481 cgtgttcttc acactgaccg tcgggttagg taacgttccc tatagcgaag aagtggtctt
     6541 tgccgggtcc atggccatcg tgctgtttct gatgttcagg ctgacacagg aactggaacc
     6601 ggacaagcga ttcaccttgg ttggtacagc agtcgtcgtt ttcatctttc gcgccatccc
     6661 gggccccgga ccgggcacga cctggtggat gattgacgag ttgagattcg atcaacagtt
     6721 tctttccgtg ttatcgctca tcgggagtac gttgaccttg gtcggcatgt ttgtctttcg
     6781 ccgcttcatg gcagaacggt cgatcgccta tgtcgtcggc tttctcacaa tcgtcggcac
     6841 gattctctcg cttcccattc tcggcatgta ttacggcttg cacgaatgga cagcgcgtct
     6901 cacgggcggc gtcatcgatg cgcggtttat cgcgttgatc gataccgctc tcgaatcccc
     6961 gctcggacag atctccatga ttccgatgct ggcttggatc gccaattccg ctccggctca
     7021 cttaaaagca acattctttg ccgtgatggc ctcgtttacg aaccttgccc tctccttgag
     7081 ccagctcggg acgaaatatc tcaacgagat ctatgtcgtt acccgggagg tcagggatcc
     7141 ggcagcgggg gccatccgaa cgcccgccga ctatagtcaa cttggcgatt tgctgattgc
     7201 gcagttgttg ctgggtttgg ccctgcctct tgccgcgatt atgttcgcac ggatcacccg
     7261 cttcaagagc gcttagatcc agagagcaag attgtctcga tcaagaatcc tggtctgttt
     7321 cggaagaggc caacgccttt gccaaacatc tcctaatgga gacaagatga agaagatagg
     7381 ccatcgggac gagcaccgtg agcagccaga cgacagggaa gtaggcaatg ataaacgtgg
     7441 gaatggagag gtgaagaagc tgagacggat agggaaccga cagcaatcca tgcacgagaa
     7501 cgaccgtcac gagacttaag cccatcaggt tccagatgag cacgacgtgc cgcagagagt
     7561 tctcgccgcg gcgcttgagc caaatcgcca agccgacggc ggtaattcct tctaggacat
     7621 caaagttcct tccgtagtac gtcatttcaa tggcgagcag cttccttgtg gccagggttg
     7681 cgagaaggag ttcgaccgga atgcgaaagc attgaagacc caccagtatg tattgcggta
     7741 gctccgccaa caattcgctc cactgtgaaa accaaccgag ccaaaggacg aaggcaagtt
     7801 gcgccaggac aagt
//
LOCUS       DASTQY010000196         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1223293, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000196 DASTQY010000000
VERSION     DASTQY010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223293"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFR82_06550"
     CDS             complement(<1..396)
                     /locus_tag="VFR82_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU4501294.1"
                     /translation="MGLSDNFYRLKRLPPYVFAQVQSLKLEARHRGEDIIDFGMGNPD
                     QPTPTHIVDKMIEAARKGKNHRYSASRGITKLRHAIAAWYQRNYDVDLDPETEAIGTI
                     GSKEGLAHLSLAMIGPGDVVLTPTPTYPIH"
     gene            674..1387
                     /locus_tag="VFR82_06555"
     CDS             674..1387
                     /locus_tag="VFR82_06555"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEU4501295.1"
                     /translation="MARLGVNIDHVATLRQARGGQDPDPLAAAVLVELAGADGVVVHL
                     REDRRHIQDRDLHLLREIIKTKLDMEMAADETMAKIALALKPDLVTLVPEKRQELTTE
                     GGLDVLTHKERVQQIITLMHDSGIPVSLFIEPDLGQIKTAHKVAADFVELHTGRYANA
                     KRSKEADAEFEAITQAAKLAYKLGMGVNAGHGLNYQNVKRLTHIPEIVEYNIGHSIIA
                     RAVLVGLDQAVREMKALLG"
     gene            1446..>2009
                     /locus_tag="VFR82_06560"
     CDS             1446..>2009
                     /locus_tag="VFR82_06560"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEU4501296.1"
                     /translation="MKIVTGIQMQALDRRTILEARVPSLILMERAGIGLADFIEGRFG
                     PLRGKRVAVVCGKGNNGGDGFVAARRLRQHHAAVTVLLLSAATDLSRDAAAMFRRWRR
                     IAGPSATKPFRCSEQIESILRTSDVIVDALLGTGLSTAVTGVYGEAILMMNRAGKPVI
                     AVDIPSGIGADTGAVLGQAVRATATVSF"
BASE COUNT          421 a          553 c          590 g          445 t
ORIGIN      
        1 gtggatggga taggtcggtg tcggcgtcag cacaacgtcc cccggaccga tcatggccag
       61 cgacaaatgg gcgagacctt ctttggatcc gatcgtcccg atcgcttccg tctccgggtc
      121 gagatcgaca tcatagttgc gctggtacca ggcagcaatg gcgtgacgga gttttgtgat
      181 gcctcgtgag gccgaatagc ggtgattctt gcctttcctg gcggcctcga tcattttgtc
      241 cacgatatga gtcggcgtgg gttgatcggg attgcccatc ccgaagtcga tgatgtcttc
      301 gccacgatgc cgcgcttcca gcttcaggct ttgtacctga gcgaacacgt agggcgggag
      361 ccgcttgagc cggtagaaat tatctgacaa tcccatggct gctgctggcg aacggctcac
      421 cggggaggac aaaccgctct gacgttaaga tggaggccta ttgtaattgc gaggaagttg
      481 ctgagtcaaa ttatcttgtg attctgtggg gttccgcaca gcaatctcgc gaaggttgtg
      541 accaagactt gccccatcgc cctcacgcag ccttgcccct ctaggcggct ttctgttaca
      601 acatcggcat ccgcaccatt tcgaattgtt ctgcctgtga cgctcctcac cagatgactt
      661 caacgggagg gttgtggccc gactcggtgt gaacattgat cacgtggcca ccctgcggca
      721 agcccgcggc gggcaggatc ccgatccgct ggccgcagcc gtgcttgtgg agttagccgg
      781 cgccgacggt gtcgtcgttc atttgagaga agatcgccgc catattcagg atcgtgatct
      841 tcacctgctg cgcgaaatca tcaagacaaa actcgatatg gaaatggctg ccgatgaaac
      901 aatggccaag attgccctgg ccctgaagcc ggatctggtg actctcgttc ctgaaaagcg
      961 tcaagaattg acgactgaag gcggactgga cgtgctgacc cacaaggaac gcgtgcagca
     1021 gatcattacg ttgatgcatg acagtggcat tcccgtaagt ctgttcatcg agccggatct
     1081 cgggcaaatt aaaacagcgc acaaggtcgc cgctgatttt gtagaattgc atacgggtcg
     1141 atatgccaat gccaagcgct cgaaagaagc agacgcggaa ttcgaagcca tcacgcaggc
     1201 cgccaaactt gcctacaagc tgggaatggg ggtcaacgcc ggccatgggt tgaattatca
     1261 gaatgtcaag cgattgaccc acatccctga gatcgtggaa tacaatatcg gccacagtat
     1321 tattgctcga gctgtcctcg tcggccttga tcaggctgtg cgcgagatga aagcactgct
     1381 cggctgaccc caagacgtgc tcttcggaag accgcaggtc tcctcaatga ttgcttgagc
     1441 accccatgaa aatcgtcacc gggatccaga tgcaggcctt ggatcgccga accattctag
     1501 aggcacgcgt gccgagcctc atcctcatgg aacgcgcggg aattggattg gccgatttca
     1561 ttgaaggccg gtttggaccg cttcgcggga agcgtgttgc tgtcgtctgc gggaaaggca
     1621 acaacggcgg ggatggcttt gtcgcggctc gccggttacg ccagcatcat gcggcagtga
     1681 cagtattgct actgtcagcg gcgacagatt tgagtcgaga tgccgccgcg atgtttcgac
     1741 gctggcggcg catcgccgga ccttccgcga ccaaaccctt ccgatgctca gagcagattg
     1801 aatcgatcct ccggacaagt gacgtcattg tggatgcgtt gcttggaaca ggcctctcca
     1861 ctgctgttac tggcgtctat ggcgaggcca tcctgatgat gaaccgagcc gggaaacccg
     1921 tcattgccgt cgatatccct tccggtattg gcgcagacac gggcgccgtt ttggggcagg
     1981 ccgttcgtgc gacggctacc gtatcattc
//
LOCUS       DASTQY010000197         4290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1449734, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000197 DASTQY010000000
VERSION     DASTQY010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449734"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1477
                     /locus_tag="VFR82_06565"
     CDS             218..1477
                     /locus_tag="VFR82_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HEU4501297.1"
                     /translation="MEIIDAATWGGNGNQLNAGNGGDPLNHNGTSTGTGINGNGGRLG
                     VRAAYMLVRNFAGIQGLSVKAGRQYVIFGNHSLFGHFDWANTGYSHDGIMLQYSTKAW
                     DVYGGWFRNSEGDLSQAAPVGSLAGPIAGANTNGQDGGRDADMFIFYNQIKSVPGFLI
                     EPFYVYYSNRYASEDNRAQGLGTAKKSNQSRHMVGNRIEMRKGNFDFINETAYQFGAM
                     GDTGGTNAVQNGYGQQRNLHINAWATRNWIGYTHYQSSWKPRFAVNFDYASGDGRANC
                     NISAAGGPCSSANTFENFFPTNHIHMGYMDIQAWKNMMSPSGNFQARPTKDDHIEIWY
                     THLNLANARDNWYRGAQGAYIFSKAGNTARHIGQEVDFTWTHMFMDGKLAFQATYGHL
                     FSGSYIEQNLGTGNDQQWAYVQLWMNF"
     gene            complement(1587..2207)
                     /gene="thrH"
                     /locus_tag="VFR82_06570"
     CDS             complement(1587..2207)
                     /gene="thrH"
                     /locus_tag="VFR82_06570"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HEU4501298.1"
                     /translation="MQKPVITCLDLEGVLVPEIWINVALKTGIEELKMTTREMPEYDA
                     LMKRRLAILDQHKLTIADIQEVIAGMGPLEGASAFVTWLRERCQVIILSDTFYQFALP
                     LMRQLGFPTLFCNQLEIDGKGKIVSHHMRMQNQKKHSVAALKSLNFFTMAAGDAYNDT
                     AMLAEADAGFFFRPPDHLPKEFPQFPVTQTYQELQKRFAEAGKLNG"
     gene            complement(2247..3047)
                     /locus_tag="VFR82_06575"
     CDS             complement(2247..3047)
                     /locus_tag="VFR82_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEU4501299.1"
                     /translation="MAAPSAERFDVALNTPAGRLNTVLDVPTGFVPITSIVPLTRRLG
                     EDMLKLEEHQSREAGQSISCRMGCAACCRMLVPLSPPEAFALRAYVEQLPQDRQAALM
                     QRMAASKAALASQGLLDRLEAVANATTPIPDEDLEPLNRAYYALRHPCPFLENEICSI
                     YEARPAACRELLVTSPPELCDNLAENPVQPIPVAVRIGTVLGLLWASLTNTSARLIPL
                     PLALDWAAQHRGDTTRTWAGSYLIDQMLDKMWRVLSQEFERRGQSPRL"
     gene            3343..3522
                     /locus_tag="VFR82_06580"
     CDS             3343..3522
                     /locus_tag="VFR82_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501300.1"
                     /translation="MGLPEDVLHEMWSLCNEARDHLTDQVWLDRNVSDQPASKAKVRE
                     LTYHALIKFLEAKQR"
     gene            3519..4202
                     /locus_tag="VFR82_06585"
     CDS             3519..4202
                     /locus_tag="VFR82_06585"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU4501301.1"
                     /translation="MRGRLHAPKIPAFRLRALLSLLAIGATALVAYTASRPSEAGVEL
                     VPVATNGLQLPLFLTHGGDGSGHLFVVEQPGTIRVIDQGVLREAPFLDLRDRVWTKGN
                     EQGLLGLAFHPEHRGNGRFFVNYNRREDGATVVAEFTRNGRSLDVSAETERILLVVPQ
                     PYLNHNGGMMAFGPDGMLYIGRGDGGSKGDPQNRAQNPQELLGKILRIDVNRGQPYAV
                     PRDNPFVTG"
BASE COUNT          926 a         1153 c         1305 g          906 t
ORIGIN      
        1 gatgcgcgcg gacctccgtg tgcggccgga aatgcggaac aacgtttgct tcggcggcgg
       61 tgcgccggtt ggcggagcgt gtaacgggcc ggcggccatc gctgcagcac cgggagctcg
      121 caccgcgaat gcaggcacaa aagccaatga tgcgtatgtc caacagtggg ttcggttggg
      181 cttgggctat gatctgtcac cagacgtgaa cttttacatg gaaatcatcg atgccgccac
      241 ctggggaggg aacggcaacc agctcaacgc gggcaacggc ggtgatccac tcaaccacaa
      301 cggtacatcg acaggaaccg gtatcaacgg aaacggtgga cgtcttgggg ttcgtgcggc
      361 atacatgctg gtccgtaatt tcgccggcat ccagggcctc agcgtgaagg ccggccggca
      421 atacgtgatc ttcggcaacc actcgctgtt cggccacttc gattgggcca acacaggtta
      481 cagtcatgac ggtatcatgc tgcaatacag taccaaggcc tgggatgtgt acggaggatg
      541 gttccgcaat tcagagggag atctaagcca ggcagctcct gtaggcagct tggcagggcc
      601 aatcgccggt gctaacacga acggccagga cggcggtcgg gatgccgaca tgttcatttt
      661 ctacaaccaa atcaagtcgg tgcctggttt cttgatcgaa ccgttctatg tgtactattc
      721 gaaccggtat gcatcagaag acaaccgtgc acaaggtctg ggaacggcaa agaagtcgaa
      781 tcaaagccgc cacatggttg gtaaccgcat tgagatgcgg aagggcaact ttgacttcat
      841 caatgagacg gcctaccagt tcggagcgat gggcgataca ggtggtacga acgctgttca
      901 aaacggttac gggcagcagc ggaacctgca catcaacgct tgggcgacca gaaactggat
      961 cggctacacg cattaccagt catcctggaa gccacgcttt gccgtcaact ttgattacgc
     1021 ctccggcgat ggccgcgcca actgtaacat cagcgcagcc ggtggaccct gtagctcggc
     1081 gaacacgttc gaaaacttct ttccgacaaa ccacatccac atgggttaca tggatatcca
     1141 agcctggaag aacatgatga gcccatcagg caacttccaa gcgcgtccga cgaaagatga
     1201 tcatatcgag atctggtaca cgcacctgaa cttggcgaac gccagggaca actggtatcg
     1261 tggcgcccag ggtgcgtata ttttctcgaa ggccggcaat actgcgcggc acatcggtca
     1321 ggaagtcgac ttcacctgga cccatatgtt tatggatggc aaactggcct tccaggccac
     1381 ctatggccat ctgttctcag gctcgtacat cgagcagaac ttgggaacgg gcaacgacca
     1441 gcagtgggcc tatgtccagc tttggatgaa cttctaaaga gagttgcagc tacgctgtag
     1501 gcgtaaggtg cgatccgcct gatgcctcaa aagaaagcct cgtggaggga ccctccatga
     1561 ggctttctct ttggttggtt tgcccgctac ccgttgagtt tgcccgcctc agcaaagcgt
     1621 ttctgcagtt cctgataggt ttgcgttacg ggaaactgag ggaattcttt gggcagatga
     1681 tcgggcgggc gaaagaagaa gccggcgtct gcctcggcaa gcatggccgt atcgttgtag
     1741 gcatccccgg cggccatggt aaaaaaattc agagacttca gggcggcaac cgagtgcttc
     1801 ttctggttct gcatgcgcat gtgatgagac acaattttgc cttttccgtc aatctccaat
     1861 tgattacaga acagggttgg aaagccgagt tgacgcatga gcggcaacgc aaactgatag
     1921 aaggtgtctg acaggatgat gacctgacaa cgctcacgaa gccaggtgac aaacgcgctg
     1981 gcaccctcca aaggtcccat gccggcgatc acctcctgga tatcggcgat ggtgagcttg
     2041 tgctgatcca ggatggccag ccggcgcttc atcagcgcat cgtactctgg catttcgcgt
     2101 gttgtcatct tcaattcttc aatgccggtt ttcagcgcaa cattgatcca aatctcggga
     2161 actaagacgc cttccaggtc caagcacgta atcaccggct tctgcattga cgattctcct
     2221 ttgtttgcaa tcacggcagg atggctttaa agcctgggcg actggccgcg tcgctcgaat
     2281 tcctgactga ggacacgcca cattttatcc agcatttggt cgatcaggta tgagccggcc
     2341 caggtgcgtg tcgtgtcgcc ccgatgctgt gcggcccagt ccaacgcgag cgggagcgga
     2401 atgagtcgag cggaagtgtt ggtcagactg gcccatagca ggcccagcac cgtcccgatg
     2461 cgaacagcga cggggatggg ttgcacggga ttctccgcca ggttgtcaca gagttccggg
     2521 ggggaggtga cgagcagctc ccggcaggca gccggtctcg cttcatagat tgagcagatc
     2581 tcattctcca gaaatggaca gggatggcgc aaagcatagt aagcccggtt gagcggctct
     2641 aagtcttcgt ccggaatcgg tgtcgtggca ttggccaccg cttccagtcg atccagcaat
     2701 ccctgtgagg ctagtgcagc tttggaagct gccattctct gcatgagggc ggcttgccgg
     2761 tcctgtggaa gttgttcgac gtacgctctg agtgcgaagg cctcaggggg ggagagggga
     2821 acgagcatcc ggcagcacgc ggcgcacccc attcggcagg aaattgactg ccctgcctcg
     2881 cgcgactgat gctcctccag tttcagcatg tcttcgccga ggcgccgcgt gagcgggacg
     2941 atcgaggtaa ttggcacgaa acccgtgggg acgtctagca ccgtattgag acggcccgca
     3001 ggggtgttca gcgcaacgtc aaaacgttcg gcggagggtg ctgccatcgg tgcatcatag
     3061 aaaaccgcca accggttggt caattgaaca ctggtgcgtg cgtctgggtt gctctccctt
     3121 cggcttcgcc gcataatgcg ccagaggaga atgggcatgg cgctctcggg ccacgtcgtg
     3181 ggccttttga aggaatatat gcaggatctt gtcgtccagg ccgagcagga ggcggcagcc
     3241 caccagtcgt ttggctttta gcagacatcc tatcgaccgg accaggccat ctcagatctg
     3301 ctggccattc tcgacgatcg gatcgaatct gaaggcgtcc aggtgggact gcccgaagat
     3361 gtgttgcatg aaatgtggag cctgtgcaac gaggcgcggg atcacctgac ggaccaggta
     3421 tggctcgatc gaaatgtgag cgaccaaccg gcttcgaagg ccaaggtccg tgaattaacc
     3481 tatcacgcac ttataaagtt tctcgaggct aaacagcgat gaggggacgc cttcacgcgc
     3541 ccaagatccc tgccttccgt ttgcgggctc tcctgtcgct attggcaatt ggcgcgaccg
     3601 cgcttgtggc gtatacggcc agccgaccct ctgaagccgg ggtcgaactc gtccccgtgg
     3661 cgacgaatgg cttacaattg ccattgtttt tgacgcatgg gggagatggc tcggggcacc
     3721 tgtttgtcgt cgaacagcct ggcacaattc gggtcatcga tcagggtgtt ctcagggaag
     3781 cgccctttct ggatcttcgg gaccgtgtgt ggacaaaagg caatgagcag ggtctcttag
     3841 gtttggcgtt tcatcctgaa catcgcggca atggccggtt ctttgtgaac tacaaccggc
     3901 gtgaggacgg tgcgacggtt gtggcagagt ttactcgcaa tggacggagt ttggacgtgt
     3961 cggccgagac cgaacgcatt ctcctggtcg tgccgcaacc ctatctcaac cataacggag
     4021 gaatgatggc gttcgggcca gatgggatgc tgtacatcgg tcgcggagac gggggctcga
     4081 aaggcgatcc gcaaaatcgg gctcagaacc ctcaggagtt gctggggaag atcctgcgga
     4141 tcgacgtgaa tcggggccag ccctatgcgg tccctcggga taatcccttt gtcactggat
     4201 agggtcgacc ggaggtgttt gccttgggag tcaggaaccc ctggcgattt tcatttgatc
     4261 gggaaaccgg cctgctctgg ctggccgatg
//
LOCUS       DASTQY010000198         9072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_444742, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000198 DASTQY010000000
VERSION     DASTQY010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444742"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VFR82_06590"
     CDS             complement(<1..502)
                     /locus_tag="VFR82_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501302.1"
                     /translation="MKDAEARSDWVFGLSLVQVILLLFFAATLIYVTQNVDVSGQEPP
                     SGSGETNHQDISVQARLDAATKKNNALEQELGDMSGLVDELKFMVGAKVSSKEGFREA
                     IETLKRGYALCQKDDNTLIEATIRQGAENVQVIGEIPSDLFVNLAKGDSTSDLDQIVS
                     FIQDVYE"
     gene            complement(489..1736)
                     /locus_tag="VFR82_06595"
     CDS             complement(489..1736)
                     /locus_tag="VFR82_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEU4501303.1"
                     /translation="MHRLPTIPLERPMPVEDELEDLPPSSDDSALTHLKLATAWLCAT
                     LAGVAAIAYATISQTSPPLTMVALAVLLVAYTGYGYFIPRKNTIQFADSLYYMGFLWA
                     IFALIATFVIWPAPKLTTEAILTSFGYALGTTFCGMFLRLLLIQFRDDTAPDRLEVAE
                     KKIERRVAALIQEINEATMEMATFRDRAAGDLGGTMRDLVQSLTAVREKIAEQHRTMA
                     KAMSDGFESSLKEILGRLSAIQIPQDCLTGEVAKLISTLGKQGESFQKAAQRLETSLL
                     RAAETVTSFGDSLHGSEGAKQVGIAVNDLSMKIKERSEQFLKMTTAFEQSRTELDSQL
                     SSLQSLRSAASMVATRLSAFENELVELSSASISAEVKTGLMSVQQAIRSSLEGSKAIE
                     SMMRDVLLLMRQRVTEEHSGERR"
     gene            complement(2062..2265)
                     /locus_tag="VFR82_06600"
     CDS             complement(2062..2265)
                     /locus_tag="VFR82_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501304.1"
                     /translation="MNDTDLSRVLSLIITVLTDPYDQFIEKLRADGRITDEEVQELHL
                     ALKEQIPKLSELIKPHPDQSAET"
     gene            complement(2306..2629)
                     /locus_tag="VFR82_06605"
     CDS             complement(2306..2629)
                     /locus_tag="VFR82_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501305.1"
                     /translation="MTPNQTTKSWKRIPDGTRVRLRENAQEGVIDGLTELVIGPARNP
                     DGRTQYRVNLGDPERMLVSEDALLIVVDKEGLVFILKEKAEYRRLITEQLRGGFSADR
                     FVPLN"
     gene            complement(2664..3920)
                     /locus_tag="VFR82_06610"
     CDS             complement(2664..3920)
                     /locus_tag="VFR82_06610"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4501306.1"
                     /translation="MDTSVQLSFREIGVSEALIKDLAKAGLSSPTPIQAQAIPAALVG
                     RDVIGCAQTGTGKTAAFVIPVIERLARLPKGEPQALILAPTRELALQILKTIETLGHR
                     LGISATVIVGGADMQAQIRGLRQRPDILVATPGRLLDHMWNGTVNLLPIKILVLDEAD
                     RMLDMGFAPQISQILTALPEERQTLLFSATLPSDLTQFVQASVNDPVQVMVTPSATTA
                     DGVTQALHYTTHTGKQELLLSLLNQEKETVLIFTRTKHRAEQVGHVLSRAGHRVGILH
                     GDRSLSQRRSALDGFKRGTFRVLVATDIAARGIDVANIGHVINFDLPYCPEDYVHRIG
                     RTARMKATGRATSFITSEDPQQLRAIERLLGHELPLASGSPQPASSQPGQTRSRRHGR
                     ASSGRPVRSRSHVHDNHPMTQGNVLQ"
     gene            4305..4643
                     /locus_tag="VFR82_06615"
     CDS             4305..4643
                     /locus_tag="VFR82_06615"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU4501307.1"
                     /translation="MPNKQASLDRIFYALADTTRRSVLQRLSTRQAPVSELAEPFDMA
                     LPSFMQHLDVLEDCGLVRSQKAGRVRTYQLRPYSLKAAEKWLAKHRTMWETRLEQLDD
                     YLTTMEEQTR"
     gene            4640..5158
                     /locus_tag="VFR82_06620"
     CDS             4640..5158
                     /locus_tag="VFR82_06620"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU4501308.1"
                     /translation="MTRPIVQPDPRLDLVFERIVDVPQELVWKAWTTPEHLKPWFTPS
                     PWKTVDCEIDLRPGGLFRTVMRSPEGQEFPNVGCYLEIVKNEKLVWTNALLPGYRPAP
                     PLAADAPTCDSFPFTAILMLERHQKGTKYTALVIHRDEAGRKQHEEMGFREGWGKALD
                     QLVEYSKGQLTL"
     gene            complement(5612..6316)
                     /locus_tag="VFR82_06625"
     CDS             complement(5612..6316)
                     /locus_tag="VFR82_06625"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEU4501309.1"
                     /translation="MDVPQQTATPVKVHVSEAVGDVEALLLRPTDARSLLVLAHGAGA
                     GMHHSFMESLACELAGFGVATFRYQFPYMQQRRGRPDAPALLTATVQAAVKAASDAAS
                     DLLLLAGGKSLGGRMTSLAFADGRSTVRPTAPVRGLVFFGFPLHPPGRPGTQRADHLT
                     AVQMPMLFLQGTRDAFADLTLLRPVCTKLAPHATLHVVAAADHSFHVLKRSGTTDADV
                     IAELARTVASWANTLA"
     gene            complement(6401..7117)
                     /locus_tag="VFR82_06630"
     CDS             complement(6401..7117)
                     /locus_tag="VFR82_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501310.1"
                     /translation="MPYGQNLSGCGLVSVLLFVSLANTNPLVWAGQIETSDRNPSVLV
                     LDPPSLKQPPVADGIVDRYLLLPRGDVSGLLLRDGAQMHVTNRAAQELIKAVQPGDHV
                     RVHGRRASDSPLVKPDVIVNVTDGTSFTVPYRLDLPIPPSEDRPTVNEMKAAGRITVL
                     LYDPLKGVVNGVVLSDGTQVRLPPDVGERFHASLQPDMEVEVEGFGTSTRHGNALEAT
                     AIGQKGRPLTHLDASIQQLR"
     gene            7281..7817
                     /locus_tag="VFR82_06635"
     CDS             7281..7817
                     /locus_tag="VFR82_06635"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HEU4501311.1"
                     /translation="MRMCNCLQRRISYLALALMITVTLWGCSAVPRRYIWIAEPGVTL
                     TMLSTHQQSYVGKVVLLGGTIIEEEEHEQFVLLRLKNRPLDQDYKPHRPADTGSSEGG
                     SYWVMVSKGQVPLKYRDWARATVVGRVTGEHRLRSEPVLMLLYMRGWGASGNHAGVWE
                     NIDPNYVPGVPGSIDMRN"
     gene            8096..8557
                     /locus_tag="VFR82_06640"
     CDS             8096..8557
                     /locus_tag="VFR82_06640"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU4501312.1"
                     /translation="MTALELKIPPVALMLLFALMMWLMSTYETSLSFLLPWRRAIAVF
                     VACAGLAIGLGGVVAFRRAKTTVNPVTPEATTTMVTSGLYRFSRNPMYLGLLVVLIGW
                     AIFLSHPLAFALLPLFVLYMNRFQIIPEERILSAKFAHQFTAYKETVRRWV"
     gene            8645..>9072
                     /locus_tag="VFR82_06645"
     CDS             8645..>9072
                     /locus_tag="VFR82_06645"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HEU4501313.1"
                     /translation="MTWLHGMLFITLALSISGCAATKQARTVERLGFLKDLYPRMTEG
                     DEHAGESLLIYKNPKVPNIPPNSYTKIMLDPVLFFRGPQSKMQGISQHQAQLMADTFY
                     AVIYQEVSKDYEMVDKPGPGTLRVQVAMTHLEESWPMLDVV"
BASE COUNT         2003 a         2587 c         2477 g         2005 t
ORIGIN      
        1 attcatacac atcttggatg aacgacacaa tttggtcgag atctgacgtg ctgtccccct
       61 tggccaaatt cacaaataga tcggaaggaa tttctccgat cacctgaacg ttttccgctc
      121 cttgacgaat tgtcgcctca atcagcgtgt tatcatcttt ctgacacagg gcatacccgc
      181 gcttcagggt ttcaatcgcc tctcgaaagc cttccttaga tgaaactttg gcgcccacca
      241 tgaactttaa ctcgtcaacc aacccgctca tatccccaag ctcttgctcc aaagcattgt
      301 tcttcttcgt tgccgcatcg agccttgcct gaacactgat atcttgatga ttcgtctcac
      361 cggatcctga aggcggctct tgcccgctga cgtccacatt ttgggtcaca taaatcaacg
      421 tcgcggcaaa gaacaggagt aggatcactt gaaccagtga caggccgaac acccagtctg
      481 atcgcgcttc agcgtctttc accggaatgc tcctcggtca ctcgttgtct catcaagagc
      541 agtacatcac gcatcatcga ctcgatggct ttgctccctt caaggctcga acggatagcc
      601 tgctgcacac tcatcaggcc ggttttgact tcagccgata tcgaagcaga cgataactca
      661 accagttcat tttcaaatgc cgacaaacgt gtggcgacca ttgaagccgc cgatcgcagc
      721 gattgcaggc tgctgagctg actgtccagc tcagtccggc tctgctcgaa ggcggtggtc
      781 attttgagaa actgctcgct cctttctttg atcttcatag acagatcatt gacggcgatt
      841 ccgacttgct tcgccccctc cgatccatgc aacgagtcac caaatgacgt cacggtttcg
      901 gctgcgcgca acaaactggt ctcgagtctc tgcgccgcct tttggaagct ttccccctgc
      961 ttccctaacg tcgagatgag cttggccacc tctccagtaa gacagtcttg aggaatttgt
     1021 attgccgaga gcctgccgag aatctctttc agagacgact caaagccatc gctcattgcc
     1081 ttggccatcg tccggtgttg ctcagcaatc ttttcgcgga cagcggtcaa tgactgcacc
     1141 aggtcccgca tcgtgccacc gagatcaccg gctgcgcgat ctctgaacgt cgccatttcc
     1201 atcgtcgctt cgttgatctc ttgaatgaga gctgccacgc ggcgctcgat cttcttttcg
     1261 gcaacctcaa gccgatccgg agccgtgtcg tcccgaaatt ggatcagcag cagccgaaga
     1321 aacattccac agaatgtagt ccccagcgca tatccgaagc tcgtaagaat cgcctcggtc
     1381 gtcaactttg gagctggcca gattacaaac gtggcgatca gggcaaagat cgcccagagg
     1441 aaccccatgt agtagagcga gtctgcaaat tgaatggtgt tcttccgtgg gatgaaatag
     1501 ccgtaaccgg tgtaggccac caacagcacg gcaagcgcca ccatggttaa tgggggcgag
     1561 gtttgtgaaa tcgtcgcata ggcgatggcg gcaacaccag ccaaggtcgc gcataaccat
     1621 gccgttgcga gtttaagatg agtgagagcc gaatcgtcgc ttgatggcgg aaggtcttcc
     1681 agttcgtcct cgaccggcat tggcctttcg agaggtatgg tgggaagccg atgcataact
     1741 ccctccccat ggttatacgg aagctctctg ttcggcctta tcttctggcc tagccctagc
     1801 tcctataact ggggattccc ctagcagcgc ctgcgccttg ttgcagctac cagtaccggc
     1861 cgatacgtaa agtgtggcta aatttcaatt ggttaattaa tattaattaa gttattgttt
     1921 atgttctgcg agcgcgcgcg gttttgtcgt cgccgtcttc ccgatgtctc ttcggcaagt
     1981 cggcacaatg atggttaagc atgtggtgct gggatagtcc ctgctaccct gagcggcgag
     2041 aacacaactt gccgctcgtt gtcaggtttc tgccgattgg tccgggtggg gttttattaa
     2101 ctcggacaac ttaggaattt gctctttgag ggccaggtga agctcttgca cttcctcgtc
     2161 cgtgattctg ccgtcagcac gcagtttctc aatgaactga tcatagggat ctgtcagcac
     2221 cgtgatgatc aaactgagaa cgcgactgag gtccgtatca ttcatgcatc tggttctcga
     2281 tcgtccggag ttgctgaagc tccgctcaat ttaaggggac aaacctgtcc gctgaaaacc
     2341 ccccgcgcag ctgctcggtg atgaggcgtc ggtactcggc cttttccttc aggatgaaga
     2401 ccagcccttc cttgtctacc acgatgagca gcgcatcttc cgacacgagc atccgttccg
     2461 gatctccgag attgactcga tactgcgtcc gcccatcagg atttcttgca ggtccgatca
     2521 ctaactccgt cagtccatcg attacgccct cttgggcgtt ctccctgaga cgtacccggg
     2581 ttccgtcggg aattcttttc caactcttcg tcgtctgatt tggcgtcatg atgccccctt
     2641 tcacgatgta ttacgctgat ccgctattgg agaacgttgc cctgagtcat gggatggttg
     2701 tcgtgaacgt gcgagcggct ccgtactggc cgccctgagg aggcccttcc atgtcgccga
     2761 gaccttgtct ggcccggctg actggaagca ggctgcggac ttccggaggc cagtggcaac
     2821 tcatggccaa gaaggcgttc gatcgcacgg agctgctgcg gatcttcgga cgtaataaaa
     2881 ctcgtggccc gtccggtcgc cttcatccgg gccgtccggc cgattcgatg gacgtagtcc
     2941 tcaggacaat agggtaaatc gaaattgatc acgtggccga tattggccac gtcgatgccg
     3001 cgtgccgcaa tatcagtcgc gacgagcacg cgaaacgtcc cccgcttaaa gccatcaagg
     3061 gcacttcggc gctgtgacag actccgatcg ccatgcagga tgccaacacg atgtcctgca
     3121 cgactcagca catgccccac ctgctctgcc cgatgcttgg tccgagtaaa gatcaggaca
     3181 gtctccttct cctggttcag cagagaaagg agtaactcct gcttgcccgt atgcgtcgta
     3241 tagtgcaacg cttgcgtgac accgtccgct gttgtggccg acggcgtcac catgacctgc
     3301 accgggtcat tgacgctcgc ctgtacgaac tgcgtcagat cggatggcaa tgtggcggag
     3361 aacagcagtg tctgccgttc ctccggtagc gcagtgagga tttgactaat ctgcggagca
     3421 aaccccatgt ccagcatcct gtcggcttcg tcaagaacca ggatcttgat aggaagcaaa
     3481 ttgacagtgc cattccacat gtgatcgagc agccgcccgg gcgttgcaac aagaatatcc
     3541 ggccgctgac ggagccctcg tatctgggct tgcatgtcgg cgccgcccac aatgacagtc
     3601 gcggaaattc caagacggtg accgagtgtt tcgatggtct tgagtatctg gagcgcgagc
     3661 tctcgagtcg gagccaatat caaggcttgc ggctcccctt tcggcaatcg agccaaccgt
     3721 tctatgaccg ggatgacaaa tgcggcggtt ttcccggtcc cggtttgagc gcacccgatc
     3781 acgtcacgcc cgaccagcgc cgcaggaatc gcctgggctt gaatcggagt gggggatgaa
     3841 agccctgcct tggcaagatc cttgatcaat gcctcggaca caccaatctc gcgaaaactt
     3901 aattgaacag acgtatccac tacacaatcc tttcagtgtg atcgcatgat gacgggatca
     3961 gagaaccgag gggagaaaca accaggaagg gtgccgctcc agactggacc tggtcgcgat
     4021 gcgatcgcga agtccgtgat gctctgcttg tcccatgccg gactcatacg agggagcagc
     4081 cggacacagc atacaggacc ctctcgtcgc agtcaatgta ttgcgaactt ctccgagcct
     4141 ctcaggactg ctcatcctga cgttggtctg aatccgccca tgcgcaagct cagcgaggaa
     4201 cgttcacgaa tggggcagag gttaagcccg tatagctgtt cctgcccggt tccctccttg
     4261 ctcggccatt gacttcccgt tcgtgaaaca ctacagttag gcacatgcct aacaaacaag
     4321 cttctcttga tcggattttt tatgccttgg ctgacacgac cagacgctcc gtacttcaac
     4381 ggctcagtac gagacaggct ccggtcagcg agctcgccga accgttcgat atggcgctgc
     4441 cgtcattcat gcagcacctg gacgtcttag aagactgtgg tttggtccgc tcgcagaagg
     4501 caggccgcgt gcggacctac caactgagac catactcgct gaaggcagct gaaaaatggc
     4561 tggcgaagca tcgcacaatg tgggaaaccc gccttgagca attagacgac tatctcacca
     4621 ccatggagga gcagacacga tgactcgccc gattgtgcaa cccgatcccc ggttggacct
     4681 cgtcttcgaa cgcattgtcg acgtcccgca agaactggtc tggaaagcct ggaccacgcc
     4741 cgagcatctg aaaccctggt ttaccccgtc cccgtggaaa acggtcgatt gcgaaatcga
     4801 ccttcgtccg ggcgggctgt ttcgaacggt catgcgttct ccggaaggac aggagtttcc
     4861 caacgtcggc tgttatttgg agatcgtcaa gaacgagaag cttgtctgga cgaatgcgtt
     4921 attgcccggt taccggcccg ctcctccact cgcagcagat gcgcctacct gcgactcttt
     4981 tccgttcacg gcaattctga tgcttgaacg ccatcagaaa ggaacgaaat acacggcgct
     5041 ggtgattcat agggatgagg ccggccgtaa gcagcatgag gagatgggat tccgggaagg
     5101 ctggggcaag gctctcgatc agctggttga atattcaaag ggacagctca ccttatgact
     5161 ggcggccttt tcgcgtaagc ttgccagcgc caatcacgac ggttgccaag gagctcgcaa
     5221 tggcgaaacc gcaaatcggg cgcgtagggt tgctgattgc tctcgtggcc atgctgagta
     5281 ctgaaatggc attggcgcgg ccgaatgaca gccccgccgg tagcctgcca ttgcctgatg
     5341 agacgggtgc aatcgttcat ggaagattga ccgagtgcgg cgcagttaca agaccggtgt
     5401 tccaacagga cagcgacgag cagcgaggtc cggtcgacgg cgtcatcgtg gccgaagaca
     5461 tgaggtgccg gcaatgccat gggaaatgct cagccgacag tttaagatgc cggagtcagt
     5521 gcgcaagcga cagcccctgt ttggttcact gtgaagagag aacgcgaaac tgcgagtcta
     5581 tgtgcaagca gattttccaa tgcgagtgag cttaagcgag cgtattagcc cacgaagcca
     5641 ccgtgcgcgc gagctcagcg atgacatcag cgtcggtcgt ccccgatcgt ttcagcacat
     5701 gaaacgaatg gtctgcggca gccaccacat gtaaggtggc atgaggtgcc agcttcgtgc
     5761 agacaggacg taacagcgtg agatcagcaa acgcatctcg cgtgccctgg agaaacagca
     5821 tgggcatctg caccgctgtt aaatggtcgg cgcgttgcgt gccgggacgg cctggtggat
     5881 gtagagggaa cccgaagaag acgaggccac gcaccggcgc cgttggccgc acagttgatc
     5941 gtccatcggc aaacgccagc gaagtcatcc ggccccctaa cgatttacct ccagccaata
     6001 agagtaaatc ggatgcggca tctgaggctg ctttcactgc cgcctgtacc gtcgccgtca
     6061 ataatgccgg agcgtcgggc ctgccccgtc gctgctgcat gtacggaaac tgatagcgaa
     6121 aggtcgccac accgaagccg gcgagttcgc acgcgagact ctccatgaag gagtgatgca
     6181 tccccgcccc tgccccgtgt gctaagacca gaagcgatct cgcatcggtg gggcggagca
     6241 gcaacgcctc gacgtcgccg accgcctcgg acacatgcac tttcactggt gtcgctgtct
     6301 gttgcgggac atccattccc ttttcttggc tttttgaaag acagagcgtt agccggttgg
     6361 cgcacgagcg tctatcacag atttaaccca tcgagaccac ttagcgaagc tgttgaatag
     6421 aggcatcaag atgagtaagc ggccggcctt tctgaccgat tgccgttgcc tccagtgcgt
     6481 tcccatggcg ggttgaggtg ccgaagcctt ccacttcgac ctccatatca ggctgtaacg
     6541 acgcgtgaaa tcgctccccg acatccggag gtaaacggac ctgtgtccca tccgacaaca
     6601 ccacgccatt cacgacgcct ttcaagggat catagagtaa cactgtaatt cttccggcag
     6661 ctttcatttc attgacggtt ggtcgatctt ccgatggagg aatgggcaga tcgagccgat
     6721 agggcaccgt gaatgaggtg ccgtcggtga cattgacgat cacgtcgggt ttcacgagcg
     6781 gtgaatccga ggcacgccgt ccatgcaccc gcacatgatc accaggctga accgctttga
     6841 tgagttcttg ggcagcccga ttcgtgacgt gcatttgcgc gccgtcgcgc aacaacaagc
     6901 cactgacatc accgcgaggc agcaacaaat agcggtccac aatcccatcg gccacgggag
     6961 gctgtttcag ggagggagga tcgagcacga gaaccgatgg gttccgatca gaagtctcaa
     7021 tctgtccggc ccaaacgagc ggatttgtat tagcaaggct gacaaacagt aggacagaaa
     7081 ccaaaccgca ccctgacaga ttttgtccat atggcatgtg atcacctacc agccttgagg
     7141 tagcatgacc attgtggagc aagatgctgg aaggcatcaa gaggaagcac ctcctcccct
     7201 cccgtaggca aactagaccg cctgcctaca aacacgtaag atgcgcccag cgcgtagcca
     7261 ccccaacgag gacagttcct atgaggatgt gcaattgcct gcaacgacga atctcctacc
     7321 ttgcattggc cttgatgatc acagtcacgc tttggggatg ctcggctgtc ccgaggcggt
     7381 atatctggat cgctgagcct ggcgtcacgc tcaccatgtt gagtacgcat caacaatcat
     7441 atgtcggaaa ggtcgtgctg ctggggggaa ctattatcga agaggaagaa catgaacagt
     7501 ttgtcttatt gcggctcaag aaccgcccac tggatcagga ctacaagccg caccgccccg
     7561 cagacacagg aagctcagaa ggcggttcct actgggtcat ggtgtcgaaa gggcaggtcc
     7621 ctctcaagta ccgagactgg gcacgcgcaa cggtagtcgg gcgggtgacc ggcgaacacc
     7681 gactccggtc cgaaccggtg ctcatgctgc tttacatgcg aggatggggc gcgagcggga
     7741 atcacgcggg agtgtgggag aacatcgacc ccaattatgt accgggggtt ccaggcagca
     7801 tcgacatgag aaattaggcg cgttcgtatt cgagagggtg cgtgtactgc ggtgcgtcac
     7861 ggtcatgtga tggacggcgc cgccattaga tgatgacgca ctcgacacca ggacagagga
     7921 aacgggcgag tgcacgtcag cacccaacca gtcccgttgt ggttcatgcc ccatcagtac
     7981 atgagcgaga cgcattcttc ctcatcactc ttcacaggca cttgtgccag tagacggcag
     8041 agaaatgcgg tggcagagac cgccttatcc ccgctcttag cgtagagggc cgttgatgac
     8101 agctttagaa ctcaaaatcc cgccggtggc gctcatgctg ctcttcgctc tgatgatgtg
     8161 gctcatgtca acatacgaaa cttcactttc tttcctcttg ccctggcgac gtgccatcgc
     8221 cgtctttgtt gcctgtgccg ggctggcgat cggcttgggc ggagtcgtgg cttttcgccg
     8281 ggcaaaaacc acagtcaacc cggtcacccc agaagcgacg acaacaatgg tcacttccgg
     8341 tctgtaccgc ttcagccgca atccgatgta tctgggcctt ctcgtagtcc ttatcggatg
     8401 ggcgattttt ttgtcccacc ccctggcctt tgcactgctt cctctgttcg tgctgtacat
     8461 gaatcgcttt caaatcattc cggaggaacg gatcttatct gccaagtttg cgcatcaatt
     8521 cacggcatac aaggagaccg ttcgccggtg ggtatgatgc tcaagagcac gactgacgac
     8581 ggccatgaat gatgtgctaa tcacgtagac aatagagccc taacagagac ccgaggagga
     8641 attcatgaca tggctgcatg ggatgttatt catcactctg gctctctcga tatccggatg
     8701 cgcggccacc aaacaagcac gcacggtgga gcggttagga tttctgaagg atctctaccc
     8761 tcgcatgacg gaaggcgatg agcatgcggg agagtcgttg ctgatttata agaatcccaa
     8821 ggtgcccaat atcccaccaa acagctatac caagatcatg ctcgatccgg tgttgttctt
     8881 tcgcggcccg caatcgaaaa tgcagggcat ctctcagcat caggctcagc tcatggccga
     8941 cacgttttat gcggtgatct atcaggaagt ctcaaaggat tatgagatgg tcgacaaacc
     9001 tggcccgggc acgctgcggg tgcaggtcgc catgacgcat ctggaagagt cttggcctat
     9061 gttggatgtc gt
//
LOCUS       DASTQY010000199         7106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1079429, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000199 DASTQY010000000
VERSION     DASTQY010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079429"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..597
                     /gene="rpsI"
                     /locus_tag="VFR82_06650"
     CDS             205..597
                     /gene="rpsI"
                     /locus_tag="VFR82_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU4501314.1"
                     /translation="MAVATEYATGRRKCAIARAWVTGTAGDITVNEQPLEKAFPRLTL
                     RQIIQLPLEMAGVSGKFSIKATVHGGGPTGQAGALRHAIARALVALSQPLRAPLKKEG
                     LLTRDSRVKERKKYGQKGARKRFQYSKR"
     gene            818..1891
                     /gene="argC"
                     /locus_tag="VFR82_06655"
     CDS             818..1891
                     /gene="argC"
                     /locus_tag="VFR82_06655"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEU4501315.1"
                     /translation="MRKIRIAIAGASGYAGAELVRLAALHPYFEVVTVTSEKSAGTPV
                     ATVFPSLAGTVALTFQSLAPEALAQQADAVFLALPHTKSLAPVAACAETGTFVVDLSA
                     DYRLKDPAAYERWYQTAHTQAHLLKSAVYGLPELHREAIAKAKLVASPGCYPTAAILQ
                     LAPLFAQDLVQPHTVVIDAKSGISGAGRSPALPYHFPEAHESLEPYKIGQHRHIPEIE
                     QELQALLQRHAGSTKVDGDSSTVTFTPHLVPMNRGILSTAYCRLKKPLPLEDVRALYH
                     TFYKNDRFIRLSEDIVPNPRYIKGSNYCDIGVYADQRAGWLVTVAALDNLVKGAAGQA
                     IQAMNLMLGLPEEIGLTAPAAYP"
     gene            1924..3132
                     /gene="argJ"
                     /locus_tag="VFR82_06660"
     CDS             1924..3132
                     /gene="argJ"
                     /locus_tag="VFR82_06660"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEU4501316.1"
                     /translation="MSQNDSRGITAPQGFRAAGLHCGIKQAGLLDLALVVSDRSGPIA
                     GVFTTNQVVAAPVTLDRLHLRHGVGRAILINSGNANACTGRPGMAAAQRTAAVVARIL
                     GTPIHEVFIGSTGVIGRPLPVDRIVKAVPDLFKRLSSLGGLSAARAILTTDLKPKAIA
                     RRARINGRTVIIGGMAKGSGMIHPAMATMLGYLTTDAAITRTALQRALTQAVDASFNC
                     ISVDGDTSTNDTVLCLANGLAGNRLIREGTPPFRQFVRLLTEACQPLALAICRDGEGV
                     TKVVRIDVEGAGNTLQARQVATTIGTSNLVKTALFGEDANWGRVMAAIGRAGVAVDPA
                     KIDVSFGGVPMVRKGIGLGPAAERRIAKVFHRKEFTIQVKLRQGRHRSHLWTTDLSYD
                     YVRINASYRS"
     gene            3357..4130
                     /gene="rpsB"
                     /locus_tag="VFR82_06665"
     CDS             3357..4130
                     /gene="rpsB"
                     /locus_tag="VFR82_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU4501317.1"
                     /translation="MGVVSIKELLEAGVHFGHQTNRWNPKMKKYLFGERNGIYIIDLQ
                     QTVARMEQAYAFVRDTVASGQSVLFVGTKRQAAEILQEEAVRSNMFYVNQRWLGGMLT
                     NFQTIRRSIDKMKKLEGMLADPAQHGLTKKEIAQKQKEIVKLQKFLTGIKNMRGLPGA
                     VFVLDTRVERIAVQEATRLEIPVIAIIDSNCDPDNITYPIPGNDDAIRSIKLITSRIA
                     DACIEGAHLRTQRDEAEFHSSPAAEDKAATVSLAGAPAS"
     gene            4188..4784
                     /gene="tsf"
                     /locus_tag="VFR82_06670"
     CDS             4188..4784
                     /gene="tsf"
                     /locus_tag="VFR82_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEU4501318.1"
                     /translation="MAGASQLVKELREKTGAGILDCQKALQESGDDIEKAIDYLRQKG
                     LAAAQKKSGRETNQGLIHAYIHMGGKIGVLIEVNCETDFVARNEEFKAFVNDLALQVA
                     AAKPSYVKREDIPKEVVDKERAIYEGQAKEMGKPPAAWPKIVDGKLEKFYQESCLMEQ
                     AFIKDPAVMVKDLLAQKIAKIGENMNIRRFTRYQLGEA"
     gene            4781..5506
                     /gene="pyrH"
                     /locus_tag="VFR82_06675"
     CDS             4781..5506
                     /gene="pyrH"
                     /locus_tag="VFR82_06675"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU4501319.1"
                     /translation="MSSAKYRRILLKVSGEMLAGEQGYGIQPSVLEGLAAEIAEVVSL
                     DIQVAVVIGGGNIFRGIAASASGMERASADYMGMLATVLNALALQNALERSGVMTRVQ
                     SAIEMRQLAEGYIRRRAIRHLEKKRVVIFAGGTGNPYFSTDTAAALRAMEIGAQVIMK
                     GTKVDGIYDADPAKNPGAKKYTEISFLSILNQHLAVMDSTAISLCMDNQLPLVVFNLK
                     IPGNFKRVALGEQIGTIVTVDKR"
     gene            5524..6087
                     /gene="frr"
                     /locus_tag="VFR82_06680"
     CDS             5524..6087
                     /gene="frr"
                     /locus_tag="VFR82_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU4501320.1"
                     /translation="MSNSAAIRQSVTGKMEGALEHLRRDLAGLRTGRASVALLDNVRV
                     DYYGTMTPLRQVANLSTPEARLITIQPWEPQLIKEVEKALSISGLGITPSNDGKIIRV
                     PLPPLTEERRKELSKICKKHGEDTKVHIRGFRRDANEELKKLQKDAKLTEDELRKSEA
                     EIQKLTDQYVQKIDDAIKKKEQEILEV"
     gene            6292..>7106
                     /gene="alr"
                     /locus_tag="VFR82_06685"
     CDS             6292..>7106
                     /gene="alr"
                     /locus_tag="VFR82_06685"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU4501321.1"
                     /translation="MHTTYASIDLAALGRNLSGLQTRLHRDCGVMAVVKANAYGHGAV
                     EISRALMERGIARLAVASVQEGVALRHAGITADIVVLADLFDEHCEELLAYRLKPVIT
                     DLRLLPTLAKKAETATHPFPIHLKIDTGMGRLGFPPSEFEKVLDALPSWRSLTIEGLM
                     THLADSDGEDTGHTERQLELFRTVLDGCKRRSMTVPMIHAANSAALIRYPHSHFSLVR
                     PGIALYGYHTLPEAVQYNELNPILSLRSTVIQLRTIKPGESISYNRTFIAKRPS"
BASE COUNT         1616 a         2121 c         2038 g         1331 t
ORIGIN      
        1 gtgggcgcca cccatccgca ccaggcacag aatccggaac cgatcacgct caattgattt
       61 gagcgaggca agaacgagcc cggcgaaagg cgcgtcccgg gaaatcgatt gcaaagcaat
      121 cggtgggctg ggcgggtgaa aacagtcgcc ggggcgggcg ggtgaaaagc acgattttca
      181 gcatccttta aaggaggaga gagcatggca gtagcaaccg aatacgcgac aggacgaagg
      241 aaatgcgcaa tcgcccgcgc ttgggtgacc ggcacggcgg gtgacattac cgtgaatgaa
      301 cagccgctcg aaaaggcatt tcctcggctc acccttcgcc agatcattca gctcccgctc
      361 gagatggccg gagtctctgg caaattttca atcaaggcga ccgtgcacgg cggtggcccg
      421 acgggacaag ccggtgcgct gcggcatgca atcgcccgag ccctggtggc cttgagccaa
      481 ccgcttcgcg ctcctctcaa gaaggaaggt ctgctcaccc gcgactcgcg cgtaaaagag
      541 cggaaaaagt acggccaaaa gggagcgaga aagcggttcc agtactcgaa gcgctaagcg
      601 tcggagccag catttggaaa aagggaaggc ttccggcctt cccttttttt atgtctacag
      661 ctataaacac actgcgttat ataaggagga tgatgaaaca gttcttccag caaggccaca
      721 cgacgcgaac cccgagaacg cagcctggtg agaggcgcgt ctcggcgcga cggggtcggg
      781 cgggtgagga cattggcttt tacgtcatcc gtactccatg agaaaaattc gcattgccat
      841 cgcaggagcc agcggctatg ccggagccga actcgtccgc cttgccgcgc tccatccata
      901 ctttgaagtc gtcaccgtga cctcggagaa atccgcagga acccccgtcg ccaccgtctt
      961 tcccagcctg gcgggcacgg ttgccttgac ctttcaatcg ctcgctccag aggccttggc
     1021 tcaacaggcc gatgccgtgt ttctcgccct tccacatacc aagtccttgg ctccggtcgc
     1081 ggcctgtgcg gaaaccggca ccttcgtcgt cgacttgagt gcggattatc gcttgaaaga
     1141 tccggccgcc tacgaacgct ggtatcaaac cgctcatacc caggctcatc ttctgaaatc
     1201 cgcggtctac ggactaccgg aattgcatcg cgaggccatt gccaaggcaa agctggtcgc
     1261 atcgcccggg tgctacccga cagcggcaat tttacaattg gcgccgctct ttgcccagga
     1321 tctcgtgcag ccgcacacgg tcgtcattga cgccaagtca ggcatctccg gcgccggaag
     1381 aagcccggcg ctgccctatc attttcccga ggcgcatgag tcgcttgagc cctacaagat
     1441 cggtcagcat cgccatattc cggaaatcga gcaggaactt caggcgctgc tgcaacgcca
     1501 cgccggctcc accaaggtgg atggcgattc cagtactgtg acctttacgc cgcatctcgt
     1561 cccgatgaat cgcggcattc tcagcacagc gtattgccgc ttgaagaagc ccctgccgct
     1621 cgaggacgta cgcgcgctct atcacacgtt ctataaaaac gatcgtttca tccggctctc
     1681 tgaagacatt gtgcccaatc cccgctacat caaagggtcg aactattgcg acatcggtgt
     1741 gtatgccgat caacgggccg gctggctcgt gaccgtcgcg gccctcgaca atctggtcaa
     1801 aggtgccgcc gggcaggcga ttcaagcgat gaacttgatg ctgggacttc ccgaagagat
     1861 cggcctgacc gcgcccgccg catatcctta accctccggt catccttcaa tgcgcaggtc
     1921 tccatgagcc aaaacgactc tcgtggcatt acagcgcctc aaggctttcg tgccgccggc
     1981 ctgcattgcg gcatcaagca ggcggggtta cttgatctcg cgctcgttgt ttctgatcgc
     2041 agcggtccga tcgccggcgt gttcacgaca aatcaggtcg tcgcggcacc ggtcacgctt
     2101 gaccgcctgc atctgcgcca cggcgtcggg cgtgccattc tgatcaacag cggcaatgcg
     2161 aacgcctgca caggccgtcc gggcatggct gcggcccaac gcactgcggc ggtcgttgca
     2221 cgaatattgg ggactcccat tcacgaagtc ttcatcggct cgacaggagt catcggacgc
     2281 ccgcttcccg tggaccgcat cgtcaaggcg gtccccgatt tattcaagcg cctcagcagc
     2341 ctaggaggac tgagtgcggc ccgtgccatt ctcacgaccg atctcaagcc gaaagccatc
     2401 gcccggcgag cgcgcatcaa cggccgaacg gtcatcatcg gagggatggc gaagggctcg
     2461 gggatgatcc atcccgctat ggccacgatg ctgggctacc tcacgaccga tgcggcgatc
     2521 acccgtaccg ccctgcaacg ggcgctgaca caggcggtcg atgcatcctt caattgcatc
     2581 tccgtcgacg gagacaccag caccaatgac acggtgctgt gtctcgccaa cggcctggcc
     2641 ggcaatcggc tgattaggga gggaaccccg cccttccgcc agtttgtgcg cctgttaact
     2701 gaggcctgtc agccgttagc cctggccatt tgccgtgacg gtgaaggcgt caccaaagtc
     2761 gtcagaatcg acgtcgaggg ggccggcaat accctccagg ccaggcaggt cgccaccacg
     2821 atcggaacgt cgaacctggt caagaccgcc ctgttcgggg aagacgccaa ctggggtcgc
     2881 gtcatggcgg ctatcggcag ggcgggagtc gcagtggatc ccgctaagat cgacgtgtcc
     2941 tttggaggcg tgccgatggt caggaaggga atcggtcttg gaccggccgc agaacgaaga
     3001 atcgccaagg tctttcatcg caaggagttc acgatacagg tgaagttgag gcagggccgt
     3061 caccggtccc atctgtggac gacggacttg tcctacgact acgtgcggat caatgcaagt
     3121 tatcgatcat aaggccactt gccttgacct gaaaccttgt aaactccggg gcattgtgct
     3181 aacgttccaa ggcttttggg ccgacgtatc cagccccctc ttccgcctcg cggcgagtac
     3241 gggggaaatt attaaaatcc acatcggcgt cttcgacgtc gcttggcttg agaataaggg
     3301 agcgtactcc ctcgccgtca cacggcgctc tgcaggcctt gaggggaggt gaaggtatgg
     3361 gagtcgtgtc aatcaaggaa ttgttggaag ccggtgtcca tttcggacac cagaccaatc
     3421 ggtggaaccc gaagatgaag aaatacctct tcggcgaacg caacggcatt tacatcatcg
     3481 atctgcagca gacggtcgcc agaatggaac aggcctatgc cttcgtccgt gataccgtcg
     3541 cctcagggca gtctgtgctc tttgtcggca ccaagcgtca agcggcggaa atcctccagg
     3601 aagaagcggt ccgctccaac atgttctacg tgaaccagcg gtggctcggt ggaatgctca
     3661 ccaatttcca gaccatccgg cgcagcatcg acaaaatgaa aaaattggaa ggcatgctgg
     3721 ccgatccggc ccaacacggc ctgaccaaga aagagatcgc acaaaaacag aaggagatcg
     3781 tcaagctgca gaagtttctc accggcatca agaacatgcg aggccttccg ggcgccgtct
     3841 tcgtgctcga cacgcgggtc gaacggattg cggtccagga ggcaacccgg ttggagattc
     3901 cggtcatcgc catcatcgac agcaactgcg atcccgacaa catcacatac ccgattcccg
     3961 gcaacgacga cgcgattcgc tcgatcaagc tcatcacgtc gcgcatcgcg gacgcctgca
     4021 tcgaaggcgc ccatttgcgc acgcaacgcg acgaggccga attccactcg agtcccgccg
     4081 cagaagacaa agccgcgacg gtatcgctgg ctggcgcccc cgcgtcataa ccatcgtttc
     4141 gagcgaccgc acacatccat cgattccacg aagaaggacg attgcgcatg gctggagcaa
     4201 gtcagttggt caaagaattg agagagaaaa cgggcgccgg cattctggat tgtcaaaaag
     4261 cgctgcagga gtccggcgac gacatcgaaa aggccattga ttacctcaga caaaagggcc
     4321 tggccgctgc ccaaaagaag tccggccggg aaaccaacca ggggttgatt cacgcctata
     4381 ttcatatggg cggcaaaatc ggcgtgctca tcgaagtcaa ctgtgagacg gacttcgtgg
     4441 ccaggaacga agaattcaag gccttcgtca atgacctggc cttgcaggtt gccgctgcca
     4501 agccctcata cgtgaagcgc gaggacatcc ccaaggaggt cgtcgacaaa gaacgcgcca
     4561 tttacgaagg tcaggccaaa gagatgggca agccgccggc cgcctggccg aagattgtgg
     4621 acgggaagct ggaaaagttt taccaagaaa gctgcctgat ggaacaggcg ttcatcaaag
     4681 accccgccgt catggtcaaa gacctgctcg cccagaagat tgccaagatc ggcgagaaca
     4741 tgaatattcg ccgcttcaca cgctatcaac taggcgaagc atgagctctg cgaaataccg
     4801 gcgcatcctc ctcaaagtca gtggagagat gttggccggt gagcagggct acggtatcca
     4861 gccctccgtc ctcgaaggcc tcgccgccga aatcgccgag gtggtctccc tcgacattca
     4921 agtggccgtc gtcatcggcg ggggtaacat cttccgcggc attgccgcca gcgcctccgg
     4981 gatggaacgg gcttccgccg actacatggg catgctggcc accgtgctca atgcgttggc
     5041 gctccagaac gcgttggaac gctctggcgt gatgacgcga gttcaatccg cgatcgagat
     5101 gcggcagctg gccgaagggt atattcgccg cagggccatc cgccatctcg aaaaaaagcg
     5161 ggtcgtgatt ttcgccggag gcacgggcaa tccttacttt tcgacggaca ccgccgccgc
     5221 gcttcgcgcc atggagatcg gggcccaggt catcatgaaa ggcaccaagg ttgacggcat
     5281 ttacgacgca gacccggcga agaatcccgg ggcgaagaaa tatacggaga tctcgttcct
     5341 ttcgatcctg aaccaacatc tcgcagtcat ggactcgaca gccatcagtc tctgcatgga
     5401 taatcaactg ccgctggtcg tcttcaatct gaagatcccc ggcaatttca agcgggtagc
     5461 cctcggtgaa cagatcggga cgatcgtcac cgtggataaa cgctaatttc ttgcggagtc
     5521 gttatgtcaa actcagctgc cattcgacaa tctgtgacgg gaaagatgga aggcgcgctg
     5581 gagcacttga gacgggactt ggccgggttg cgaacgggac gggcatcggt ggcgctgctt
     5641 gacaatgtgc gtgtggatta ttacggcacg atgacgccgt tgaggcaggt tgcgaatctc
     5701 tcgacgccgg aagcgcggct tattacgatc caaccctggg agccgcaact tatcaaggaa
     5761 gtcgagaaag cgctctccat atcgggattg ggcattacgc catccaacga cggcaagatc
     5821 attcgcgtgc cgctgcctcc cctcaccgaa gaacgccgca aagaactcag caagatctgc
     5881 aaaaagcacg gcgaggacac caaggttcac atccggggct ttcgtcgcga tgccaacgaa
     5941 gagttgaaga aactccaaaa agatgccaaa ttgaccgaag acgaattacg aaaatcggaa
     6001 gcggaaattc aaaagctgac cgatcagtac gtgcagaaga tcgacgatgc catcaaaaag
     6061 aaggaacagg aaatcctcga agtctgatgg gacggccccg attcacaatc tgatagtctg
     6121 gtaaaaaggc tacttgttca cccgcccgac ctcgaagcgc ccagatgcta cattccgcgg
     6181 tcggccgcag ttaagcgatg cgagaacgaa tccccggtga agggcgcgtt gcgacgcgcc
     6241 ggggtgggcg ggtaaaaaca agaccttttt catcagtctg caaaaagtac cttgcacacc
     6301 acctacgcta gcatcgatct cgccgccctc ggccggaatc tttcaggact tcagactcgc
     6361 ctgcaccgtg actgcggggt catggctgtt gtcaaagcga acgcctacgg ccatggcgcc
     6421 gtagagatct cgcgagcgct gatggaacgc gggattgcga ggctggctgt ggcgtcggtg
     6481 caagaaggtg tggcgttacg tcacgccggc atcaccgccg acatcgttgt ccttgcggat
     6541 ctgttcgacg aacactgcga agaacttctc gcctaccggc tcaagcccgt catcaccgat
     6601 ctccgtctgc ttccgaccct cgccaagaag gccgagacag ccacgcatcc ctttccgatt
     6661 catctgaaga tcgataccgg gatgggacgt ctcggatttc cgccgtcgga attcgagaag
     6721 gtgctggatg ccttgccttc gtggagatcg ctcaccattg aaggcctgat gacccatttg
     6781 gcggacagcg acggtgagga caccggccat acggaacggc agttagaact gttccgcacc
     6841 gtgctggacg gatgcaagcg gcgaagcatg acggtgccca tgattcatgc ggccaatagc
     6901 gccgccctca ttcggtatcc gcactcacac ttctcgctcg tcaggcctgg cattgcgctg
     6961 tacggatatc acacgctgcc tgaagccgtt cagtacaacg aactgaatcc cattctctcg
     7021 cttcgcagca cggtcataca attgcggaca atcaagcctg gcgaaagcat cagctataat
     7081 cgcaccttta tcgccaagag gccgtc
//
LOCUS       DASTQY010000200        11995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_830045, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000200 DASTQY010000000
VERSION     DASTQY010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11995
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830045"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1337
                     /locus_tag="VFR82_06690"
     CDS             57..1337
                     /locus_tag="VFR82_06690"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU4501322.1"
                     /translation="MDQKRIVIIGGGFGGLTAARSLRRADVVLIDRTNHHLFQPLLYQ
                     VATAALSPSDIAWPLRTVFRSQRNVRVMMDEVLSIDRADRSICLRESPPVSFDVLIVA
                     PGSRHAYFGHEEWEEFAPGLKTMTDAVRLRQKMLSAFEEAERRLVSTGKHEPLAFVIV
                     GGGPTGVELAGALAEIGRKAMGPDFPNLRLDDLCVLLVEGGPRILPGFSPSLSAKAAA
                     ALSRMGVTIRLNSLVSAVHEEGVRIGQDFIRSVGIIWAAGNRASRLLDTLEAAQDGAG
                     RVMVNPDLTVPGDPWIFVIGDAANCRGQDGKALPGLAPVAIQQGRYVAKLIEQGAAPS
                     VRSPFIYADRGMLATIGRAKAVAQIGRIHVSGLLAWLLWCIVHIFFLIGLRSRLRVMS
                     EWIWYYVTFKPGARLLFEQPEPAHRKSPGPHPGG"
     gene            1459..1881
                     /locus_tag="VFR82_06695"
     CDS             1459..1881
                     /locus_tag="VFR82_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4501323.1"
                     /translation="MKPRVTIITIGVDDLDRSLRFYRDGLGFPTRGIVGTEYEHGAVA
                     FFDLTNGQKLAIWARKDLAHEAKVPLGPRSPSEFSLGHNVGSKAEVDAVIGQAQRAGA
                     MITDPPHEAFWGGYTGSFQDPDGHLWEIAWNPEMNPPD"
     gene            1937..2554
                     /locus_tag="VFR82_06700"
     CDS             1937..2554
                     /locus_tag="VFR82_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501324.1"
                     /translation="MRSIMVAWTLLWMGWFALSAVAAKGAGKTEVMPMVKAAPAAVAI
                     ERTGTEQDLRNQLRVKNGVTELVAARALFSQAPTGTYGFIAPQPLGMALVTQSPDLML
                     ERSAPSSNAYEIHKLADGSGMVVGFIDKALIPQVTAENRPHHIRISLSSNGSDKMPYI
                     AAVPLTKLMVDRMPGRLDAKNPDSPVVLDMDLQGTANRKSVPGLQ"
     gene            complement(2568..3767)
                     /locus_tag="VFR82_06705"
     CDS             complement(2568..3767)
                     /locus_tag="VFR82_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501325.1"
                     /translation="MKGPSIVLAGFITGTLLITSTFRPAHAQPASASSPPLSPKTETV
                     KKYLESCRNRPAQDAGCEKLRKDAVEILQEDLRTLGSTADRGYLLSIVPVFQSEEVEL
                     RIAAADAVGMIGPQDRDAEFLAPVANDPVPDVRAAVGQAISRGKGPALTLLGQRVRAV
                     NKTGRTPEIPPDASKFAMPVVPNSTYLFDSSSPELGRVSYVTRGGDAAGFFKGKAKKG
                     PFKLEEFQDKFRYQLQDEHQAFNQAQDAASKQLEAEKPDPKNMQAFAEYMKRIVSTQS
                     RSMTRTSLDLYQPNLYGLPTVYVLEERQIGQRTYPTRYVVTYQDQALRQPGYRLVWMT
                     VPDDALKSAQVASLKEEQQEAGRKKEEEAAHKRQEAMDDLIKKKDDADKKKFKKGQAD
                     LEKELGF"
     gene            complement(3808..4575)
                     /locus_tag="VFR82_06710"
     CDS             complement(3808..4575)
                     /locus_tag="VFR82_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501326.1"
                     /translation="MSYGLWHGRNLFEAMRHRQPPSDPGAMARALSVTSLCGALLLIV
                     FNTTAMGAGPCTCEDIDKMQKHLDRVNDAGEAWKEIFAWARELYRDVDMPQSNDDLNQ
                     KFVQLTSAPKSQWYDLIKQGPVKEKKTIKKVAGVSPEGEPVVDDDFKKTNCDDIIEAE
                     HVHERAHRDFYLSFPKVLEVPMTSRLLRLRAESEVESYRAHKAFLEKKLAELKLKCIT
                     KLDNSKKRELEQAAAQRQRMNEAEIRVRLLGTAQSGK"
     gene            4634..6043
                     /locus_tag="VFR82_06715"
     CDS             4634..6043
                     /locus_tag="VFR82_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4501327.1"
                     /translation="MRSAFARQSSELLLLVPLAILAYANAWSGGFQFDDFSAILENPH
                     LADAGTFIGHLDHMVRPVLYATFLFDRTLYGMNPTGYHLLNLLLHLGSGMLIYFILIR
                     AVREKTRQVPIWTALLFLVHPIHTETVTYISGRASGLMSFFYLLALFFYIKSSEAREA
                     PTMRGLYQSGAIVAFVLSIGAKETAITFPFVLLLWDLLIHRVRGGASLRAGILSRHLP
                     FWVVSLLAAAWAWSHPRYSALAHVSYNLRPLVDNGLSEAHAVIYALWLFFCPWNQNFD
                     HDLPEFHLMSQWPLPFDLLALSVLIAVAVGMAHRLPPVSFGIGWFLLQLLPTSLIPRA
                     DLLSERNLYLASIGLSLALVVLVATLLARSDEMVKHPSLVRSVGAGIACVLVVELYLM
                     TVQRNTLYRDQLSLWSDSVKKSPNKARPHNNLGHAYALHGEWDRAIDEFRIAAQLDSD
                     YVLAQENLRDAYLHRVGRR"
     gene            complement(6071..6910)
                     /locus_tag="VFR82_06720"
     CDS             complement(6071..6910)
                     /locus_tag="VFR82_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU4501328.1"
                     /translation="MDRRLLRRALVIAWVMMAHALWPGAGLTDNSLLHLPEQPVINAA
                     ALYMVELKSGRVLLEKNATLRLPPASLTKIMTALIALRSAPLQEVVKIDPRAVIHHSS
                     YSFRPGEEFLLRDLVTAMLVVSANDACEAVAWHVGGDDRRFVSMMNEHALLLGLKNTH
                     FSNACGFDAPGHYSTAADLAKLTERALQEPIFSMMVRTLVRDISTVNGTRRMSLHSTN
                     ELLADPEVNGVKTGYTSKAGRCLIATMFKDGHSLMLVGLNLRDQWKQASRLLRYGHEI
                     LQE"
     gene            complement(6923..7729)
                     /locus_tag="VFR82_06725"
     CDS             complement(6923..7729)
                     /locus_tag="VFR82_06725"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HEU4501329.1"
                     /translation="MSRLIPIVIGLVSLLSTVSADAGPPNRPQAEYSADATIQSEEGS
                     MQQRVYATPTKERKEMLTGAGDGAVQIFRYDTKVMWQVMPSERMYMEHSMMGGQGQGS
                     DPSQWTYEDTAMGEETLNGVKVTKYKTIATSTDGKKFGGFSWRTKEGINVKQDLLYKE
                     GNDKKRMMTELSNLKIGKQDPKLFEIPEGYTKLDMGGMMGMMSGAGRQGMGQPMGRPD
                     MTGTTQPSMRKQGTKPEVVTPQETPAPETETAPESEVDKATNFMKGLFGQ"
     gene            complement(7899..10580)
                     /locus_tag="VFR82_06730"
     CDS             complement(7899..10580)
                     /locus_tag="VFR82_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEU4501330.1"
                     /translation="MASRRQATPTTGPSVTAEQQRLQEDARREQHWKRWGPYLSERAW
                     GTVREDYSPFGTAWEEFPHEHARSRVYRWNEDGLAGLCDRHQYICFALALWNGHDPIL
                     KERLFGLTGHEGNHGEDVKEYYFYLDSTPTHSYMKYLYKYPQTAFPYARLVEENRRRT
                     RLEPEFELVHTGCFDENRYFDVFVEYAKASPEDVCIRVQVVNRGPDNADLTVLPTIWY
                     RNTWSWGSDIRRPRFQAGKAVDHATVIETIHDYYGYRRLLCEKQPTLLFTENETNTKR
                     LYGDVEGAPYVKDGFHDYVVNGDTAAINPDHVGSKAAAHYVFTLPSGASRSIRLRFTA
                     DASLTTFVEEDIDAVFEARIHEANEFYETLEHRVLSEDARRIQRQAFAGLLLSKQFYH
                     YDVTRWLKGDPMGPEPPPERQHGRNADWTHLYNADVVSMPDTWEYPWYAAWDLAFHCI
                     PLALVDPTFAKEQLVLMLREWYMHPNGQIPAYEWAFGDVNPPVHAWAAWRVYKIEKKR
                     TGVGDRLFLERVFHKLLLNFTWWVNRKDAEGKNIFQGGFLGLDNIGVFDRSSPLPTGG
                     RLEQSDATSWMGMYCLNMLTIALELARENRAYEDVASKFFEHFVYICRAVNNMGGKHI
                     ELWDRKDGFFYDVLHLPNGETTHVKVRSIVGLIPLFAVETLDSALIDTLPRFKHRMQW
                     FIENRPEFSQHVETQTYDGGVRRFLSLVNRHRLRSVMRYLLDEEEFLSPCGIRALSRY
                     HKDHPYVLNVMGHEHRVDYEPGESSTPQFGGNSNWRGPIWFPVNYLLIESLQKFHFFL
                     GNHYKVEYPFGSGHQCTLWQVARELSRRLISIFLRDAGGRRPVFDGTQLFQQDPHWRD
                     HLLFYEYFHGDTGAGIGASHQTGWTGLVAKLIQQSGE"
     gene            complement(10606..>11995)
                     /locus_tag="VFR82_06735"
     CDS             complement(10606..>11995)
                     /locus_tag="VFR82_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4501331.1"
                     /translation="MNDVTVIQSVVTETPIDTAKQLVTFVGGIAFLLAMNWRLCLLIL
                     VLLPLLVVVAKLFGRRLKSLSTSIQDQTAALSTLLEEVISGIRIVKSFVQTQREEARF
                     GQQVDTTLSLTMRRAGIMALFIPVISLLTFSAAAAVLWYGGRQVIDGTVSPGELFAFV
                     LFAGILIGPFSSAARVFAQIREAQGATERVFEILDTRPEVRDDPNALVLHTVSGQLTA
                     EHVSFAYDPRQTVLTDVSFEVKPGECVAIVGPTGAGKTTLMNLIHRFYDPTDGRVTID
                     GRNLRDVTLESWYRQIALVPQETILFGGTILDNIRYGNGDADEEAVKEASRAANAHEF
                     IMGFPDGYHTVVGEKGINISGGQRQRIAIARALLKNPRILLLDEATSALDSESERLIQ
                     EALERLMRGRTTFVIAHRLTTIHSADRILVLDKGRLVETGTHDELMARQGLYHYLYTV
                     RVVEAPSKTAGV"
BASE COUNT         2360 a         3485 c         3358 g         2792 t
ORIGIN      
        1 tccctgggcc tgcagcttcc ctcgtcctga gctggcgcct gcgttgagct ccaacaatgg
       61 atcagaagag gatcgtcatc atcggaggcg gctttggcgg actcacagcc gcccgatcac
      121 tccgtcgggc cgacgtcgtg ctcatcgacc gcacgaatca tcacctgttc caaccgctgc
      181 tctatcaggt ggcgaccgct gccttgtcgc cgagtgatat tgcatggcca ttgcgcaccg
      241 tttttcgctc gcaacgcaac gtccgcgtca tgatggatga agtgctgtcg atcgatcgtg
      301 ctgatcgctc gatctgtttg cgtgagagcc cgcccgtctc gttcgacgtc ctcatcgtgg
      361 cgcctggctc gcggcatgcc tattttggcc acgaagagtg ggaagaattt gcgcccgggc
      421 tcaagaccat gaccgatgcc gtaaggctac gtcagaaaat gctgtcggcc tttgaagaag
      481 cggaacgccg gctcgtctcg actggcaagc atgaaccgct cgcgtttgtg attgtcggag
      541 gcggtcccac gggcgtggaa ctggccggcg ccctggcgga gatcggacga aaggctatgg
      601 gcccggattt tcccaacttg cggctcgatg atctctgcgt gttactcgtc gagggcggtc
      661 cgcgcattct acccggtttc agtccaagtc tttcagctaa agccgcggcg gccttgtcaa
      721 ggatgggggt gaccattagg ctcaacagtc tcgtaagcgc cgtccacgaa gagggcgtga
      781 ggattggaca ggactttatt cgttcggtcg gtatcatctg ggcggcagga aacagagctt
      841 ctcgtctcct tgatacgctg gaggcggccc aagatggcgc aggtcgggtc atggtcaacc
      901 ctgatctgac tgtccctggc gatccatgga tcttcgtcat cggcgatgcg gcgaattgtc
      961 gggggcagga cggaaaagca ctgccagggc tggctcccgt cgcgatacag cagggccgct
     1021 acgtggccaa gctgatcgag cagggtgcgg ctccgagcgt ccgctcgccg tttatctatg
     1081 ctgaccgagg gatgcttgcg accattgggc gggccaaggc ggtggcacaa atcggccgca
     1141 ttcatgtctc aggactcctg gcttggctgc tctggtgcat tgtccacatt tttttcctca
     1201 tcggtcttcg cagccgccta cgcgtgatgt cggaatggat ctggtactac gtgaccttca
     1261 agccgggggc gagattactc ttcgaacagc cggagccggc gcaccggaaa tccccaggac
     1321 ctcaccccgg tggctgaccc tcctcctggt tttccggacc ggttcacgct ggccattcga
     1381 atcgcaatgg atactccatt gacaccacgt ttagtgccct cgtagcctat ccacagctgc
     1441 cagcggctgg aggaccacat gaagccacgc gtcacgatca tcacaatcgg cgttgacgat
     1501 cttgaccgct cactacggtt ctaccgggac gggctgggtt ttcctactcg aggaatcgtc
     1561 gggactgagt atgaacacgg agccgtcgcc ttctttgacc tgacgaacgg ccagaaactg
     1621 gcgatctggg ctcggaagga tctcgcccat gaggccaagg ttccactggg acctcgcagc
     1681 ccaagcgaat tctctctcgg acacaatgtc ggcagcaaag cggaagtcga tgcggtcatc
     1741 ggtcaggccc agcgcgccgg agcaatgatt accgatccgc ctcacgaagc gttttggggt
     1801 gggtacaccg gatcgttcca agatcctgac gggcatctgt gggagatcgc gtggaatccg
     1861 gaaatgaatc cgcctgatta attgttccgc acgatattcc ggtgcaaact gcagctgttg
     1921 tcggaggtgt tatctgatgc gctcaattat ggtggcatgg acactattgt ggatgggttg
     1981 gtttgccctg tcagcagtag cggcaaaggg ggcgggaaag accgaagtga tgccgatggt
     2041 aaaggccgcc ccggcggcgg tcgcgatcga gagaaccggc acggaacagg acctccgcaa
     2101 ccaattgcgg gtgaagaacg gtgtgactga gctcgtcgca gcccgtgcgc tattttcaca
     2161 ggctcccacc ggaacctacg gctttattgc cccgcaaccg ctcggcatgg ccctcgtgac
     2221 gcagagtcct gatctcatgc tggagcgcag cgctccttct tccaatgcct acgagatcca
     2281 taaacttgcc gacggcagcg gcatggttgt ggggttcatc gacaaggctc tcattccgca
     2341 ggtcaccgcg gagaaccggc ctcatcatat ccggatttct ctgagctcca atggttcaga
     2401 caaaatgccc tacattgccg ctgtgccatt gactaagctg atggtcgatc gaatgccggg
     2461 tcggttggat gcgaagaatc cggatagtcc tgtggtgctc gatatggatc tgcaggggac
     2521 ggccaaccgt aagtccgtgc caggtctgca atagcctcct gcgacgacta aaatcccaac
     2581 tccttttcaa ggtcggcctg ccccttctta aacttcttct tgtccgcgtc gtcctttttc
     2641 ttgatcaagt catccatagc ttcttggcgt ttgtgtgctg cctcttcttc ctttttgcga
     2701 ccagcttctt gctgctcttc tttcaatgac gcgacttggg ccgacttgag cgcgtcgtcc
     2761 ggcactgtca tccacacgag tcgatatccc ggctgcctga gtgcttgatc ctgataggtg
     2821 acgacgtagc gggtggggta ggtgcgctga ccgatttgcc gctcttcaag cacgtagacg
     2881 gtcggcaaac catacagatt tggctgatac aaatccaagg aggtcctcgt catgctccgg
     2941 ctctgcgtcg agacaatccg tttcatgtac tcggcaaacg cctgcatgtt tttgggatca
     3001 ggtttctcgg cttccagttg cttgcttgcg gcatcctgcg cttggttgaa cgcctggtgt
     3061 tcatcttgaa gctgatagcg gaatttgtcc tggaactcct ccagcttgaa gggacctttc
     3121 ttggccttgc ctttgaaaaa tccggcggca tcgccgcccc tagtgacata ggagacacga
     3181 ccaagctcgg gactgctcga atcaaagaga taggtactgt tgggcacgac cggcattgca
     3241 aacttactgg catcaggcgg aatctccggt gtacggcccg tcttattcac tgccctgacg
     3301 cgctgtccca acaacgtgag cgcaggtcct ttcccgcggc tgatcgcctg cccaaccgcg
     3361 gctctcacat ccggaaccgg atcattggca acgggggcca ggaactccgc atcacggtct
     3421 tgtggtccga tcatgccgac ggcgtctgct gcggcaatcc gcaactcgac ttcttcgctc
     3481 tggaagaccg gcacgatgct gagtagatag cctcgatctg ccgtggaccc gagtgtacgc
     3541 aagtcttctt gcagaatttc aacggcgtct tttctgagtt tctcgcatcc tgcgtcttga
     3601 gccggtctgt tccgacaact ctcgagatac ttcttgacgg tttcagtttt gggggaaagc
     3661 gggggggagg atgcggatgc gggctgagca tgagccggac ggaacgtgga cgtgatcagc
     3721 aacgttcctg tgatgaaacc tgcaaggacg atcgagggac ccttcatgat tgttcttcct
     3781 cctaacagtg ccgaacctcg gtgtctgtca ctttccagat tgtgccgttc ccagcaaccg
     3841 gactcggatc tccgcttcat tcatccgctg tcgctgtgcc gctgcctgct ccaattctct
     3901 tttcttggaa ttgtcgagtt tggtgatgca tttcaacttc agctcggcaa gtttcttctc
     3961 aagaaacgcc ttgtgggccc ggtaggattc cacttcagac tccgcacgta atcggaggag
     4021 ccgggaggtc atcggcactt ccaagacctt tggaaaactc agatagaagt ctctgtgcgc
     4081 tcgttcatga acgtgctccg cttcgatgat gtcgtcgcaa ttggtctttt tgaagtcgtc
     4141 gtcgacaacg ggttcgcctt ccggactgac cccggcgact ttctttatgg ttttcttctc
     4201 cttcacgggg ccttgcttga tgaggtcata ccattgagac ttcggcgcgc tggtcagttg
     4261 gacgaatttt tggttcaggt cgtcgttgga ttgtggcatg tcgacatccc ggtacagctc
     4321 tcgcgcccag gcgaagattt ctttccaggc ttctcctgcg tcgttaaccc gatccagatg
     4381 cttctgcatt ttgtcaatat cctcgcatgt gcaggggcct gctcccatgg ccgtcgtatt
     4441 gaagacgatc agcaggagcg cgccgcacag ggacgtcacg ctcagagcac gcgccatcgc
     4501 cccgggatct gaaggcggtt gcctgtgccg catggcctcg aaaagatttc gcccatgcca
     4561 aagcccatag gacatgatcc ttcctcctct ctgtgtactc atctgttaga agagcgtatt
     4621 cttgtgccca cccatgagat ctgccttcgc gcgtcagagt tccgaactgc tcctgcttgt
     4681 tcccctggct attctggcat atgcgaatgc gtggtctgga gggttccaat tcgacgattt
     4741 ctccgccatc cttgaaaatc ctcatctcgc cgatgccggc acgtttatcg gccaccttga
     4801 tcacatggtt cgacccgtcc tgtacgcgac atttttgttt gatcgtacgt tgtatgggat
     4861 gaaccccact gggtatcacc tgttgaacct gctgctccac ctcgggtcgg ggatgctgat
     4921 ctatttcata ctcatccgcg cggtgagaga gaaaacccga caggtcccga tctggactgc
     4981 gctgctcttt ctcgtccacc ccattcacac tgaaaccgtc acctacattt ccgggcgggc
     5041 ctcgggcctg atgtccttct tctacctcct ggcgctcttc ttctacatca aatcgtcgga
     5101 ggctcgagag gcgcccacaa tgcgcggctt gtatcaatca ggcgcaatcg tcgcctttgt
     5161 tttgtcaatc ggggcaaagg aaacggccat aacctttccc tttgtgcttc tgctctggga
     5221 tctcctcatc catcgagtga gaggcggcgc gtcactccga gcggggattc tctcacgcca
     5281 tctcccgttc tgggtcgtgt cgctgcttgc tgcggcatgg gcgtggagcc atcctcgtta
     5341 ctccgccctc gcacatgtga gttacaattt gcgcccgctc gtggataacg gcttgagcga
     5401 ggcccacgct gttatctatg cgctgtggct gttcttttgc ccatggaacc agaacttcga
     5461 ccatgacttg cctgagtttc acttgatgag tcaatggccg ctaccgttcg atcttctcgc
     5521 tctcagcgta ttgatcgcgg tcgccgtcgg catggctcac cgtcttcccc cagtatcgtt
     5581 cggtatcggc tggttcttgc ttcagctcct tcctaccagt cttattcccc gagccgacct
     5641 cttaagcgaa cgcaacctct acttggcatc gatcggattg tcgctggctc ttgttgtgct
     5701 cgttgccaca ctattggccc ggagtgacga aatggtgaag catccttccc tcgtccgatc
     5761 tgtcggagcg ggcatagcgt gtgttctggt ggttgaactc tacctgatga ccgtccaacg
     5821 caacacgctt tatcgagatc aactctctct ctggtccgac tcggtgaaaa aatccccgaa
     5881 caaggcgagg ccgcacaaca acctggggca cgcctacgcg ctccacggag agtgggaccg
     5941 agccatcgac gaatttcgca ttgccgctca attggattcc gattacgttc tcgcccaaga
     6001 aaatctacgt gatgcctacc tgcatcgcgt gggccgccgg tagccatcat ggtgcgtctg
     6061 ccgtagtcgc tcactcctgc aggatttcgt ggccatagcg caggagccgt gaggcctgct
     6121 tccactgatc cctgagattc agccccacca gcatcaagct gtgcccgtcc ttaaacatag
     6181 tggcgatgag acagcgtccc gctttacttg tatagcctgt tttcacgccg ttcacctcag
     6241 ggtcagcgag cagttcatta gtactgtgta gcgacatgcg cctggtgccg ttcaccgtgg
     6301 atatgtcacg gaccagcgtc cggaccatca tggagaagat cggctcctgc agcgcccgct
     6361 cggtcaactt ggccagatct gccgctgtcg aatagtgtcc cggcgcgtca aatccacagg
     6421 cgttcgagaa gtgcgtgttc tttaatccca agagcagcgc gtgttcgttc atcatcgaga
     6481 caaaccgccg gtcatcaccg ccgacatgcc aggcgacggc ctcgcaggca tcattcgcgc
     6541 tgactacgag catggccgtc accagatcac gaaggaggaa ttcttcgccg ggccgaaagc
     6601 tgtaggagga atgatggatg accgcgcgcg ggtcgatctt caccacttct tggagtgggg
     6661 cagatctaag cgcgatcagc gcagtcatga ttttcgtgag actggcaggc ggaagccgga
     6721 gggtcgcatt tttttcgagt aggacgcgtc cggacttgag ttctaccatg tacagagcgg
     6781 ccgcattgat aactggctgt tctggcaaat ggagaagaga gttatcagta agaccggcac
     6841 caggccacag cgcgtgggcc atcatcaccc acgcgatcac cagcgccctg cggagcagcc
     6901 gtctgtccat cccgtcgttc gcctactgac cgaacaatcc cttcatgaaa ttcgtggcct
     6961 tgtcgacctc tgattctggc gccgtttcag tttccggcgc cggagtttct tgtggcgtga
     7021 caacttcagg tttcgtaccc tgtttccgca tgctgggctg agtcgtcccc gtcatatcag
     7081 gacggcccat cggctgaccc atcccctgac gtcccgcccc gctcatcatc cccatcatgc
     7141 cgcccatgtc caatttcgtg tatccctctg ggatctcaaa caacttgggg tcctgcttcc
     7201 cgatcttgag attgctgagc tccgtcatca tccgcttttt gtcgttacct tctttgtaga
     7261 gaagatcctg cttgacgttg atgccctcct tcgtccgcca ggaaaagccg ccgaattttt
     7321 ttccgtccgt gctcgtcgcg atggtcttgt acttcgtcac cttgaccccg ttcagtgttt
     7381 cttctcccat cgccgtgtcc tcataggtcc attgggaagg atcactaccc tgaccctggc
     7441 ctcccatcat cgagtgctcc atgtacatgc gttcagacgg catcacctgc cacatcacct
     7501 tggtgtcgta ccggaaaatc tgaaccgccc catcgcctgc gcctgtcaac atttctttgc
     7561 gctctttggt cggcgtcgcg tagacgcgtt gctgcatact gccttcctca ctctgaatcg
     7621 ttgcgtcagc cgaatattcc gcttgaggac gattcggtgg accggcgtcc gccgacacag
     7681 tgctcagcaa actgacaaga ccgatgacga tgggaatcag gcgtgacatg gcaatctcct
     7741 cccagggtgt gcagcgaggt gtttctatga ggtgctgtgc gtggaaatca gaagtcaaca
     7801 aatatggctc gatccgcata caagccaacg ccggtatagg actaaggcta cagcggtgca
     7861 agggtagcgt gtcgctctgt gggaagtaaa gagaaccgct attctcccga ttgctggata
     7921 agtttggcga ctaaacccgt ccatccggtc tggtggctgg cgccaatacc ggcgccagtg
     7981 tctccatgaa agtattcata aaatagtaga tggtctcgcc aatgcggatc ctgttgaaag
     8041 agctgcgtcc cgtcaaatac ggggcgcctt cccccggcgt cgcgaaggaa aatactgatg
     8101 agacggcggg agagctctct cgccacttgc cagagggtgc actgatgtcc tgacccgaat
     8161 ggatactcca ccttgtagtg attgccgaga aagaaatgga acttttgtag cgattcgatc
     8221 aagagatagt tgacgggaaa ccagatcggt ccccgccagt tcgaattgcc gccgaactga
     8281 ggggtgctcg actccccggg ctcataatcc acgcggtgtt catggcccat gacgttgaga
     8341 acatacggat gatccttgtg gtatcgggac agcgcgcgaa tgccgcatgg ggaaaggaac
     8401 tcctcttcat cgagcagata cctcatgacc gagcgcaggc gatgccggtt gacgagggag
     8461 agaaaacgcc gcaccccgcc gtcataggtc tgcgtttcca cgtgttgcga gaattccggc
     8521 cggttctcga tgaaccactg catccggtgt ttgaagcgag gcagggtgtc gatgagggcc
     8581 gaatcgagtg tttcgaccgc aaacaacgga atcaatccca caatggaacg gaccttcacg
     8641 tgggtcgtct ctccgttcgg cagatgcagc acgtcgtaga aaaacccgtc tttgcggtcc
     8701 cacagctcga tgtgtttccc gcccatgttg ttcaccgcac ggcaaatgta tacgaaatgc
     8761 tcgaagaatt tactggcgac gtcctcatag gcacgatttt cacgggcaag ctccagtgcg
     8821 atggtcaaca tgttgaggca gtacatgccc atccagctgg tcgcgtcgga ttgttcgagg
     8881 cgccctccgg tcggaagcgg agagctccga tcgaagaccc cgatgttgtc caaaccgaga
     8941 aagccgcctt ggaagatatt cttcccctcc gcgtccttcc ggttgaccca ccatgtaaag
     9001 ttcaggagga gtttgtgaaa gacccgttcg aggaagagcc gatccccgac gcctgtccgt
     9061 tttttctcaa tcttatacac acgccatgcc gcccaggcgt gaacgggcgg gttcacatcg
     9121 ccgaaggccc attcataggc cgggatttgt ccgttcgggt gcatgtacca ttcgcgcagc
     9181 atcaggacca gctgttcctt ggcaaatgtg ggatcgacga gcgcgagggg gatgcaatga
     9241 aacgccaagt cccaggcggc gtaccaggga tactcccaag tgtcaggcat ggagaccacg
     9301 tccgcattgt agagatgcgt ccagtcggca ttgcggccat gctgtcgctc tggcgggggc
     9361 tccggtccca tgggatcgcc cttgagccac cgggtaacat cgtagtgata gaactgcttg
     9421 ctcaagagca gcccggcgaa ggcctgtcgc tggatacggc gggcatcttc agaaagaaca
     9481 cgatgctcca gagtctcgta aaattcgttc gcctcatgaa tgcgcgcttc gaagaccgca
     9541 tcaatatcct cttcaacgaa ggtggtcaga gacgcatcgg cggtgaaacg tagccggatg
     9601 ctgcggcttg ctccggaagg caacgtgaaa acgtagtggg ctgcggcctt gctgccgacg
     9661 tgatcggggt tgatcgccgc cgtgtcacca ttcaccacat agtcgtgaaa gccatccttt
     9721 acatagggcg ccccttccac gtcgccatac aggcgtttgg tgttcgtctc gttttccgtg
     9781 aataggagtg tcggctgttt ttcgcaaagc aaccggcggt atccgtagta gtcatggatg
     9841 gtttcgatca ccgtggcgtg atccactgcc ttacccgctt ggaagcgcgg acgtcggata
     9901 tcggaacccc atgaccaggt attgcggtac cagatggtgg gtagcactgt caggtcagca
     9961 ttgtcagggc ctcggttcac tacctgtacg cggatgcaca catcctccgg cgaggctttg
    10021 gcgtattcga cgaatacatc aaagtaccgg ttttcgtcga agcagccggt gtggacgagt
    10081 tcaaactcag gctcaaggcg agtgcgacgt cgattctctt ccaccagccg cgcataggga
    10141 aaggccgtct gtggatactt gtacaggtac ttcatgtaag aatgagtggg cgtggaatcc
    10201 agataaaagt aatactcttt gacgtcctca ccgtggttgc cttcatgccc ggtgaggccg
    10261 aagagtcgct ctttcaagat tgggtcgtgg ccgttccaga gcgcaagcgc gaagcatatg
    10321 tattggtgac gatcacaaag gccggcgaga ccgtcttcgt tccaccgata aacgcgtgag
    10381 cgggcgtgtt catgaggaaa ctcttcccag gccgttccga agggactgta atcttcacgt
    10441 acggttcccc aggcccgttc gctcagatag ggtccccagc gtttccaatg ttgttcgcgg
    10501 cgggcgtcct cttgaagacg ttgctgctcc gccgtcactg agggccctgt cgtgggagtg
    10561 gcctgtcggc gtgaagccat gcgaatcctc gagtcgcgcg cccgatcaga caccggccgt
    10621 tttcgaagga gcttcaacga cgcggaccgt atagaggtag tgatacagtc cctgccgagc
    10681 cattagctcg tcatgcgttc ccgtttctac gaggcggcct ttgtccaaaa ccagaatccg
    10741 atcggcgcta tggatggtgg tcaagcgatg ggcgatgacg aaggtcgtgc gtcccctcat
    10801 cagccgttcg agcgcttcct gaatcagccg ttcggactca ctgtccagtg ctgaggtcgc
    10861 ttcatcaagc aggagtatgc gcggattctt gagtagagct cgagcaatcg cgatacgctg
    10921 gcgttgtcca ccagagatat tgatgccctt ttctcccaca acggtgtggt aaccatcggg
    10981 aaaacccatg atgaactcat gggcattggc tgcccggctg gcttccttca cggcctcttc
    11041 gtcggcgtca ccgttgccat accgaatgtt gtcgagaatc gtccccccaa acaggatggt
    11101 ctcctgcggc accagcgcga tttgcctata ccaactctct aaggtgacgt cacggagatt
    11161 ccgcccatcg atggtcacac gcccgtcggt cggatcataa aaccgatgga tcaaattcat
    11221 cagggtagtc ttgcctgcgc cggtcggacc gacgattgcc acacattccc ccggtttcac
    11281 ttcgaacgac acatctgtca gcaccgtctg gcgtggatca tacgcaaagc tcacgtgctc
    11341 tgctgtgagt tgccccgata cggtgtgaag cacaagggcg tttgggtcat cgcggacctc
    11401 gggccgggta tccagaattt caaatacccg ctcagtcgcg ccctgagctt cacgaatctg
    11461 tgcaaacaca cgcgcggcgg agctgaacgg accgatgaga atgccggcaa agagcacgaa
    11521 ggcgaacaat tcaccaggcg agaccgtgcc gtcaatcacc tgacggcctc cgtaccataa
    11581 gacggctgcc gcagcggaaa atgtcagcag actgatgaca ggaatgaaca gcgccatgat
    11641 cccggcccgc cgcatggtga gcgagagcgt tgtgtcgacc tgctgcccga agcgtgcttc
    11701 ttcccgctga gtttgaacga aggacttgac gatccggatg ccggagatga cttcttcgag
    11761 aagcgtgctc agggcggcgg tctggtcttg aatggaggtg gaaagcgatt taagccggcg
    11821 accgaacagc ttggccacca caaccagcaa gggcagcagc acgagaatca gcagacagag
    11881 gcgccaattc atcgcgagga ggaacgcaat gccgcccacg aatgtcacga gctgtttggc
    11941 tgtgtcgatt ggagtttccg tcaccaccga ttgaatcacc gtgacgtcgt tcatg
//
LOCUS       DASTQY010000201        14187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_214940, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000201 DASTQY010000000
VERSION     DASTQY010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 14187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14187
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214940"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2867
                     /locus_tag="VFR82_06740"
     CDS             <1..2867
                     /locus_tag="VFR82_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4501332.1"
                     /translation="WQQRWKQRDKRSEFPLFINRIVTNYLIRWHDIRKSDYVGILVTD
                     QQGALVVSSIPQVEYFYGKAPWWQALVKGASQQPYVSEITFDPSFGTHVVVVAAPILD
                     DLQKTVIGAVTILLRRDTLFHSIADVTIGSTGHAMLFTSDGSPVICPILAPEEHSVKP
                     ELINVLGKLKSGWTVAEDDSHGSKDALVGFAPVRFSQSLAAGSIGGKHWVTVVRQDPK
                     ETFAPLAELVAKVLLYGLGVLAVLWGTGVLAARRIARPIQLLQGGVQEIGSGRLERRL
                     ELKTGDEIEELAGAFNEMASNLQRSFGQIEQRMREVRALEEKYRDLIEHSPEMIYQLD
                     RSGQFVHVNKTGLDKLGYQLSEMLMMKLWDVVPSGQAARVLQYLERLVSQGQTTMETV
                     LIAKDGRPIDVEIHATALFDEDRGGLVHSRAFVRDITERHRLEQELQHYTTGLEQAVS
                     ERTQELVASQARYKALFDLVADSVFMVNAAGTVVAVNKREEQALGYAESKVVGRNMHD
                     VVVPSHREALTRWLAEIGTGQRQVPTQEITVYNAAGLETPVEMDLIRVAGAGQVLVMV
                     QMRDITDRKKLERQLQIYREELEVKVRERTREIEETKQYLENLLENANDVIYTLDIEQ
                     RFTYVNSKVNVWGYRKDDLLGRPYLSLLSRRHRGRRLKNTLDIGAKQVYEVEVMTRMG
                     EARTVMVSVSPLQGVDGEILGVLGIARDMTDTKKLEQQIRNSEKLASVGKLAAGVAHE
                     INNPLAGILNCLYNLRKGTLSPARQEEYWVSMEDGVRRVQKIVRQLLDFSQQHEPEFS
                     ATDINQIVDRVLVLTNHLFAPNLIRLETVLGPALPTLMVDRHMIEQVLMNLILNAVQA
                     MKGGGVLTIRTTVAEGVCRVEVSDTGSGIPPSVLPRIFDPFFTTKSEGEGTGLGLSVS
                     LGIAERHGGKILVESEVGKGTTFTLCLPLSRERSLVERVS"
     gene            2819..4318
                     /locus_tag="VFR82_06745"
     CDS             2819..4318
                     /locus_tag="VFR82_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU4501333.1"
                     /translation="MSSAVERAVIGGKGVMKGMAILLVDDEPLSRLSMVDALEAVGYD
                     VHAVASGTEGLNVLREKSFDLVITDLRLPGADGLTVLKATKDRSPETEVVMITAHGSV
                     ESAVGAMKLGAFDYITKPFQMDELLLIVERVGRVIALRRENRDLKETLESKFCFNGIL
                     GANSQMRGVLDKIKTVAGTDSTILILGESGTGKELVANAIHQNSARNQHPLIKVSCAA
                     LPETLLEAELFGHEKGAFTGALRQRRGRFELAHRGTLFLDEIGEISPVVQVKLLRVLQ
                     ERKFERVGGNETIDVDVRVVCATQKDLRKEVSVGRFREDLFYRLNVVQVVIPPLRKRQ
                     EDIMVIADHVLQTCSVKMNKKLKGFSTVSRELLLRYSFPGNVRELENMVERAVALGRD
                     REPVQPQDLCGFQSCPFTGGTPQDACGFCSEGLTGQKRPERPLNSLISAREEFEKDYI
                     LSVLEKVEGSRTTASKILGLSRKALWEKCKRYGIPSAKGEIEEEEEKGD"
     gene            complement(4653..5747)
                     /locus_tag="VFR82_06750"
     CDS             complement(4653..5747)
                     /locus_tag="VFR82_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEU4501334.1"
                     /translation="MTQPTASTPVPAQPLPASAGMTTPIDSLEYWTAAAREVRTFRGH
                     SHGVWSVAFAPDGLTLASAGVDRLVRIWDIETGRLLRSLRGHTNDIRAVLYTPDGQAL
                     ATGSEDRTIRLWNPKTGEPLKLLFTRYDHNICSLSLSPDGLMLARGSHNKDIKIWEVT
                     TGTELMTLLGKDQYDHHWSVCVAFSPDDRHLASGMDIGKIKVWEVLPSGEEKLLHNGH
                     WQETEEDSTETRGYFIEDDGGFQKPMDYWIGAMTFTPDAKYLITGSRDQTIKMFEMPE
                     ALEHRTLKGHGGWIRALVVTPDGKVLISSGDDNTIRFWDLKSGRCFRTIKSHTGGVRG
                     LSLSADGLRLASASWDRTVKLWEGGAEPAE"
     gene            5954..6952
                     /locus_tag="VFR82_06755"
     CDS             5954..6952
                     /locus_tag="VFR82_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501335.1"
                     /translation="MELRFQPALLQEVIDSFVEKTEREGDPTYYKEFHELADPIYEKF
                     TLDDRESEFKKLYQYLFGTWGFSDIIRDAFNEYPLVKDKVGIVLVKGVLKEDQEGVDI
                     LRKWGSVEQDLARQFAEKGLKGIGIKLIPRRFYDPALTRYCRHELMHISDMLDPAFAY
                     DPDTKVGQNPGEETLILHRYRILWSLSVDSRLTAAGKEPMLRKEDRYKEFRSWYRKIP
                     AVQLKSVFEGLWQTGFFTHSELIEMATDTLRVMDRAVDVEGGEVPETENKVMLMPGFP
                     CPLCRFPTYTWVEDMGTKIETYVLDFIRENHPGWDVEFGACDRCVEVYKLRADGVM"
     gene            7037..7630
                     /locus_tag="VFR82_06760"
     CDS             7037..7630
                     /locus_tag="VFR82_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU4501336.1"
                     /translation="MATDPKYGRRDFFKDSVISVAKAAKEFSSHADAVPEKPAPALRT
                     DWLRPPGAAGEATFLERCTKCSDCLKACPHESIVIHQDGTPIIFPDQMPCYLCDDTPC
                     IAACATEALLPVGSIQEARMGVAVVNHRLCTAGQGCHACVSKCPTDALTMDFDTQRLV
                     VAVERCVGCGMCEHVCRTVNDHIAIRVTPFRLMESPR"
     gene            7852..8820
                     /locus_tag="VFR82_06765"
     CDS             7852..8820
                     /locus_tag="VFR82_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEU4501337.1"
                     /translation="MTSKQSVQGSTQTSAAVLSKSPIKDSPAVERALNRSKLYLLVSW
                     SLLYPEDDEFLDYLQCGEFVEDGRAALEALETALDGIGGDRAKQKLALMRKQFDQIEK
                     LIASECVNWQLSELQAEHRRVFSNVITLDCPPYETLFGNDHVFAQSHVMGDIAGFYKA
                     FGVELSKDIHERLDHLSVEFEFMHFLAYKESYSRCHDGAEKTQIVVDAQKKFVKNHIG
                     RWVPLFCRMLIKKADSGLFKHVADMTSDWMDFEAAFLGVTPQTYSETDYRPATFNSPE
                     GQTYECGAQDQGNELNLLLNETGAQNFMDQKEKDKDKEEDGPVGTA"
     gene            8914..9867
                     /locus_tag="VFR82_06770"
     CDS             8914..9867
                     /locus_tag="VFR82_06770"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HEU4501338.1"
                     /translation="MRVVQTHNKSVVFGILLSALIVGVMLTIGQVPLAVSQPVTIPAK
                     AVKGPIPMDGANPVWESVPGVIVPLSGQLITTPMHPNISVKSVFVKAMTNGKEVGLRV
                     EWSDQTKNDTTIGPQDFRDQAAIMFPVNTSGAPPFQCMGQSGGTTNIWRWNAEWQKDL
                     GKDSAGLWDVDDQYPGIFWDYYFEEPAGGVTYPDRIGRSLGPFNPGIWSGNIMSDPTL
                     RVSSVEDLNANGFSTLTTQAHQDVIGNGVWEPAGSVKGGGYSGPTWRVVVKRTLETGD
                     ANDVQFKGGMSVPIAFAVWDGNNIERNGMKALSTWFTLKLP"
     gene            10081..11862
                     /locus_tag="VFR82_06775"
     CDS             10081..11862
                     /locus_tag="VFR82_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU4501339.1"
                     /translation="MKVSLLFPPTWHPSQPYLSLPSLTGFLAQAGVTDVSQRDLGIEL
                     LDEVVTKSYGAGLYQDLLEKKQTLERQRTGDTGPGSAEHLARIIESLDRFPYLIDRIE
                     LAKETLRGEGFYDIEAYRESLFLIDKWLEVISTLYFPTRLTVVDNQFSTYSIYSSKDL
                     MKVIRDEQQNPYIKLFRERFLPSIIAERPGLIGVSITATSQIIPGLTLCRLIKEAAPD
                     IHLTIGGSIFTRLVDNLRRCPNLFDITDDIVVFEGETALLELVNQMAGKKDFSKVPNL
                     IYRQNGKITVNQPFYSENVNQLPAPNYDGFPLDRYLSPEPVLPVQFSRGCYYKDCAFC
                     ALTLDHQNFRQKDPGRTIEELEWLKQRYGVRNFFFTDECFALSPTKRLCQQMIERRVD
                     VKWTCEMRFEKHLTRELLTSMRDAGCLKIVFGLESFNQRIMDFMKKGIQQEWVRRIAS
                     DCVDLGIAVHCYIIVGFPTEKEEEALETMNFIVENKKLNESYGFSCQPCLFDLEKEAP
                     IMSDPGGYGIRRIMRPAAEDLSLGFFYEVQEGMTPDQAERLYQHVYEKISEVVCELPF
                     NYSMADGLLYIARAKAEERRAVTAAQT"
     gene            11888..13003
                     /locus_tag="VFR82_06780"
     CDS             11888..13003
                     /locus_tag="VFR82_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501340.1"
                     /translation="MPATATTIERITGKLSDQPLLFQYTIKLVLLVAFLELILYRLVS
                     RLGMHLSKLAADHQWIIPTFTALTEVGQWLLNVVAILLFLGLTVGMVNRVAQRGFTGL
                     TRLVVPCIALLLLLTVGFLLVPPSMLGSAIYNTVALAAIVLLMSEYLATHHDRSHRVL
                     GLTYLLGISGWLYYQIVSTFFSFSGSVAAPPMVYESHRAGEAFMVLASILVFWAYGRG
                     VSFRSRNQRQRHRAIWFWSTAAAIFLTMFFLDYLLNQYNPGLASNVRKAADGIGWIFQ
                     FGMGYTFYLPFALYMAGLLCWSYTVIKLLTMGRLAGYGLGLMFIAGYALLYSNLTLMV
                     VLGVMLMTMDRYRRDAADSVADRAVLRTTDSLVGGHV"
     gene            13026..13826
                     /locus_tag="VFR82_06785"
     CDS             13026..13826
                     /locus_tag="VFR82_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4501341.1"
                     /translation="MEETTSSEQSRAAVDPGDQPLNPDEEIAEIEKLLAAEPDDFQAR
                     CRLGELYFSKGRLDDALVEVKKSIEMAEGIRAEMNRSLAMYYSNLGTIYATKNMADEA
                     EAEFKRALDVFQYDVLALFNLGRLCADKKKYMDAKGFYERLVEITPDDPMAWYNLAGV
                     YVELDNPQVSDYNTIDAAIQCYLRTLELDPKHLEASFKLMELALNHKKSDLAIKVMED
                     AVEHNADEPLAYYNLISVYDKCKMFEQAEQARQRLKERFAKRSKTSAS"
     gene            13854..14123
                     /locus_tag="VFR82_06790"
     CDS             13854..14123
                     /locus_tag="VFR82_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU4501342.1"
                     /translation="MFGSMGITELILILFIVLVIFGAGKLPQLGEGLGKAIKGFKKSV
                     HEADAIEAEAEAAAAQQQMASPQPSISTAPAPGVEQPAAQSATRA"
BASE COUNT         3078 a         3846 c         4244 g         3019 t
ORIGIN      
        1 actggcaaca gcgctggaag cagcgggaca agcgcagcga gtttcccctg ttcatcaacc
       61 gcatcgtcac caattatctg atccgttggc acgacatccg gaagtcggac tatgtgggaa
      121 ttctggtgac tgaccagcaa ggggcactgg tcgtgagctc gataccccag gtcgaatact
      181 tctacgggaa agccccctgg tggcaggcct tggtcaaggg agccagccaa cagccgtatg
      241 tcagtgaaat caccttcgac ccctcgttcg gaacccacgt cgtggtggtc gccgcgccga
      301 tcctagacga tctccagaag acagtcatcg gagccgtgac gattttgctc cgacgggaca
      361 cgttgtttca ttcgattgca gacgtcacca tcggatcgac cggtcacgcg atgctcttca
      421 catcggacgg gagtcccgtc atctgtccca tcttggctcc ggaagaacat tcggtgaagc
      481 cagaattgat caatgtgctg gggaagctga agtccggctg gacggttgcg gaggacgatt
      541 cgcacggaag caaggatgcc ctggtgggct ttgccccggt gcggttcagt cagtcgctcg
      601 cggcgggaag catcgggggc aaacattggg tgaccgttgt ccgacaggat cctaaagaaa
      661 cgttcgcgcc gctggcggag ctcgtggcca aggtcctgct ctatggtctc ggtgtcttgg
      721 ccgtcttgtg gggtaccggg gtgctcgcag cccggcggat tgcccgaccg atacaattgt
      781 tgcagggcgg agtccaggaa atcggcagcg ggcggttgga gcgtcgactg gagctcaaga
      841 ccggggatga aatcgaggaa ttggccggtg cattcaacga gatggcttcg aatctgcagc
      901 gctcattcgg gcagatcgag cagcggatgc gggaagtgcg ggcactagaa gagaagtacc
      961 gggatctgat cgagcattcg cccgagatga tctaccagtt ggaccgcagc ggacagtttg
     1021 tgcacgtcaa taagactggt ctcgacaagc tgggctatca gctgagcgaa atgttgatga
     1081 tgaagctctg ggatgttgtc ccaagcggac aggcggcacg cgtgctgcag tatctcgagc
     1141 ggctcgtctc tcaaggtcag accacgatgg aaaccgtcct gatcgcgaaa gacggccgtc
     1201 cgatcgacgt ggagattcat gccaccgccc tcttcgacga ggatcgcggc ggactggtgc
     1261 attcccgtgc gtttgtgcgc gatatcacgg aacggcatcg cctggagcag gaactgcagc
     1321 attacaccac cggcttggag caggcggttt ccgagcgcac gcaggaactg gtcgcatctc
     1381 aggcccgtta caaagctctg ttcgacctgg tcgcagactc ggtgttcatg gtgaatgccg
     1441 ccgggactgt tgtcgccgtc aataaacgcg aagagcaagc gcttggttac gctgagtcca
     1501 aggtggtcgg acgcaacatg cacgacgtcg tcgtgccgtc gcatcgtgag gctctgaccc
     1561 gctggctggc cgaaatcggc accggtcaac ggcaggtccc gacccaggaa attaccgtct
     1621 acaacgcggc gggtttggaa acgccggtcg agatggacct gatccgtgtt gccggagccg
     1681 ggcaagtgct ggtgatggtg cagatgcgcg acatcaccga ccggaagaaa ctcgaacgac
     1741 agctgcaaat ctatcgcgaa gaactcgaag tcaaagttcg tgagcggacc agggaaatcg
     1801 aggaaacgaa gcaatatctg gaaaacctgc tcgagaacgc caacgacgtg atctatacgc
     1861 tcgacatcga acaacggttt acgtacgtca acagcaaggt gaatgtgtgg gggtatcgca
     1921 aagacgatct gcttggacgt ccgtatctct cgctgctgtc acgccggcat cgcgggcgcc
     1981 gtctcaagaa caccttggat atcggcgcca aacaggtcta tgaggtcgaa gtcatgacgc
     2041 ggatgggcga agcacggacg gtcatggtca gcgtctcccc gttgcaaggt gtagatggag
     2101 agatcctggg cgtgctcggc atcgcccgcg acatgacgga cacaaaaaag ctcgaacaac
     2161 agatcaggaa ctcagagaag ctcgcatccg tcggaaagct ggccgcaggc gttgcccacg
     2221 aaatcaacaa cccgctcgcc ggcatattga actgtctcta caacctccgc aaaggtacgc
     2281 tttcgccagc acgccaggaa gagtactggg tttcgatgga agatggtgtc cggcgtgtgc
     2341 aaaagatcgt ccggcaactc ctggattttt ctcagcagca cgagccggag ttcagcgcca
     2401 ccgatatcaa ccagatcgtc gaccgggtgc tggtcctcac aaatcatttg ttcgctccca
     2461 atctgatccg gctggaaacc gtcctgggtc ctgccttgcc gacccttatg gtcgatcggc
     2521 acatgatcga gcaggtgctg atgaatttga tcttgaacgc cgtgcaggcg atgaaaggcg
     2581 gcggagtgtt gacgatacgg accaccgtcg ccgagggcgt ctgccgggtc gaggtgagtg
     2641 ataccgggtc aggcattccc ccgtcagtat tgccgcgcat ttttgatccc ttcttcacca
     2701 cgaagagcga gggcgaaggg accggcctgg gtttgtcggt cagtctgggt attgctgagc
     2761 gacatggtgg taagatcctg gtagagagcg aggtcgggaa aggaacgacg tttacactat
     2821 gtcttccgct gtcgagagag cggtcattgg tggaaagggt gtcatgaaag ggatggccat
     2881 cctgttggtc gacgatgagc cgctgagtcg gctttcgatg gtcgatgcgc ttgaggcggt
     2941 ggggtacgac gtgcatgccg tggcgtccgg caccgagggt cttaacgtgc tgcgcgagaa
     3001 atcgttcgac ctggtcatca ccgacctgcg gttgccaggc gccgacgggc tgacggtcct
     3061 caaagccacg aaagacagat cgcctgagac ggaagtcgtg atgatcaccg cgcacgggtc
     3121 agtggaatcg gcggtgggcg ccatgaagct gggggcgttc gattacatta ccaaaccgtt
     3181 tcagatggac gagttgctct tgatcgtcga gcgtgtcggg cgcgtcatag ccttgcggcg
     3241 tgagaatcgc gacctgaaag aaacgctgga gagtaaattc tgcttcaacg gcatcctcgg
     3301 cgccaatagc cagatgcggg gggtgctgga caagatcaaa accgtggccg ggaccgattc
     3361 gaccattctg atcctgggcg aaagcgggac gggaaaggaa ttggtcgcca atgccattca
     3421 ccagaacagc gcgcggaacc aacacccgct catcaaggtc agctgcgcgg ccttgcccga
     3481 gactctgctc gaggcggaac tgttcggcca tgaaaagggt gccttcacgg gagcattgcg
     3541 gcaacgtcgc ggacggtttg aactggccca tcggggcaca ctctttctcg acgaaatcgg
     3601 cgagatatcg cccgtcgtac aagtcaagct gctgcgtgtg ctgcaggaac gaaagttcga
     3661 gcgggtaggc ggcaatgaga cgatcgacgt cgacgtccga gtggtctgcg ccacgcagaa
     3721 ggacctccgc aaggaagtgt ccgtcggacg gttccgcgaa gatctattct atcgattgaa
     3781 cgtcgttcag gtggtgatac ctccgctacg gaagcggcag gaggatatca tggtcatcgc
     3841 cgaccatgtt ttgcagacct gctcggtaaa gatgaataag aaactcaaag gattttccac
     3901 cgtctcacgg gagctcctgc tgcgctattc gtttccgggc aatgtgagag agcttgaaaa
     3961 catggtcgag cgggcggtgg cgctgggacg cgaccgtgaa ccggttcagc cgcaggactt
     4021 gtgcggcttc caatcctgtc cgtttacggg cggcacgcct caggacgcgt gcggattttg
     4081 tagcgagggt ctcactgggc agaagcggcc cgagcggccg ctcaattcat tgatatcggc
     4141 acgggaagaa ttcgagaaag actatattct atcagtgttg gagaaagtgg agggcagccg
     4201 cacgaccgcg tcgaagattc tcggactctc acgaaaggcg ctctgggaga agtgtaagcg
     4261 ctacgggatc ccctcggcga agggagaaat cgaagaggaa gaggagaagg gtgattagac
     4321 ttccaatctc agcttagcaa ttgttaacgc tgagattgaa aatttctgat gcggctctca
     4381 aaggcgacag gcttgaagtg gcctgtcgcc ttttcatttc tcttcttctc tatatctacg
     4441 tgagggtcct ggcgaaggcg tgtgtccttc ctgcggcttg cccgaaggcg cgggtaccac
     4501 acttgttggt cagatgcgac caccagctgg ccatgccgat gttcaaaggt ccattcgtgc
     4561 gaagcacgga tgaaacaagt ttcggcattg agcagacgag cgacttcgtg tggtcgcgcc
     4621 gaagtgctgc agcaggaagg acaccggctt cgctattctg ccggctctgc cccgccttcc
     4681 caaagcttca ccgtccggtc ccatgatgca ctggcgagtc tgagtccgtc cgccgagagt
     4741 gagaggccgc ggacgccccc ggtgtgagac ttgatcgtcc ggaaacaccg gccggatttg
     4801 aggtcccaga agcgaatcgt gttgtcgtct ccggagctga tcagaacttt gccgtccgga
     4861 gtcacgacga gcgcacgaat ccagccgccg tgccctttca gcgttcgatg ctcgagcgcc
     4921 tccggcattt cgaacatctt gatcgtctga tcacggctgc cggtgatgag gtacttggcg
     4981 tcgggggtga acgtcatggc gccgatccaa tagtccatgg gcttttgaaa accgccgtca
     5041 tcctcgatga aatacccgcg cgtttcggtc gagtcttctt cggtctcctg ccaatgaccg
     5101 ttgtgcagga gtttctcttc gccgctgggc aggacctccc agactttgat tttaccgatg
     5161 tccattccgc ttgcgagatg tctgtcgtcg ggtgaaaaag ccacgcacac ggaccagtgg
     5221 tgatcgtatt gatcctttcc gagcaacgtc atcaactccg ttcccgtcgt gacctcccaa
     5281 atcttgatgt ccttattatg gctgccccgt gccagcatca agccgtccgg agacagcgac
     5341 aaactgcaga tattgtggtc gtatcgggta aacagcagct tcaggggttc gccggtcttc
     5401 ggattccaca agcggatcgt tcgatcttcg ctgccagtgg ccaatgcttg gccgtccggc
     5461 gtgtagagca ccgcccggat gtcattggtg tggccgcgaa gcgagcggag cagtcggccg
     5521 gtttcaatat cccagatccg gacgagacga tcgacgccgg cgctggccaa cgtcaggcca
     5581 tcgggcgcaa aggcgacgga ccagacgccg tgcgaatgtc cacggaacgt tcgcacttca
     5641 cgggcggccg cggtccaata ttcaagagaa tcgatgggag tcgtcatgcc ggctgaagcc
     5701 ggcagtggct gcgcaggcac gggagtggat gcggttggtt gtgtcatgag tcgtaccttg
     5761 cccgaatcgt aagtggtgaa gcggccgatg acggtggctt gattgcaaaa cgccgtttcg
     5821 gcccagtata atttgcggtc gtttccgtga tcgtaagaga agcaacggcg ccaagtcaag
     5881 ccgctccgag cgccgccaaa ccgcatcacg actggggaaa gcggcccctg gtaccggaaa
     5941 aatcgaggct gatatggaac tccgttttca acctgcactg ctgcaggaag tgatcgactc
     6001 gttcgtggaa aaaaccgagc gggagggtga cccgacctac tataaggagt tccacgagct
     6061 ggccgacccg atttacgaaa agttcaccct cgacgatcgg gaaagtgagt ttaaaaaact
     6121 ttaccagtac ctctttggca cgtgggggtt ttccgacatc atccgtgacg cgttcaatga
     6181 gtatccgctg gtgaaggaca aggtcggcat cgttctcgtg aaaggcgtgc tgaaggaaga
     6241 ccaggaaggg gtcgatattt tacggaaatg gggatcggtc gagcaggacc tggccaggca
     6301 attcgcagaa aagggcctga aggggatcgg catcaagctc atccctcgcc ggttttacga
     6361 tccggcgctc acgcggtatt gccggcatga gctcatgcac atctcagata tgctcgatcc
     6421 ggccttcgcc tacgatcccg ataccaaagt gggacagaat cctggggaag aaaccctcat
     6481 cctgcaccgg tatcgcattt tatggagtct cagcgtcgat agccggctca ctgccgctgg
     6541 caaagagccg atgctgcgca aggaagaccg gtataaagaa tttcgttcgt ggtaccggaa
     6601 aattcccgcg gtgcagttga agtccgtgtt cgagggcttg tggcaaacgg gtttcttcac
     6661 ccactctgaa ctgatcgaaa tggcgaccga cacgttgcga gtgatggacc gcgcggtgga
     6721 tgtggaaggc ggtgaagtac cggagacgga aaacaaggtc atgttgatgc ccgggtttcc
     6781 atgccctctg tgtcggttcc ctacctatac atgggtggag gacatgggga ccaagatcga
     6841 aacctatgtg ctcgacttca tccgcgagaa tcatcccggc tgggacgtgg aattcggtgc
     6901 ctgcgaccgc tgcgtcgagg tctacaagct ccgggccgac ggcgtcatgt agaagcgaat
     6961 cggaaaaagg tcgggagcgt catttcgctt cggatgatga atggaagaaa atctgtgaaa
     7021 cagaagaaca gcacctgtgg ctactgatcc taaatacggt cggcgcgact ttttcaagga
     7081 ctcggtcatc tcggtcgcaa aagccgcgaa agagttttct tcccatgcgg atgcagttcc
     7141 tgaaaagccg gctccggcct tgcgcacgga ctggctgcgt cctcctggtg ccgccggcga
     7201 ggcgaccttt ctcgagcgct gtacaaaatg cagtgactgc ctgaaggcct gtccacacga
     7261 gtcgattgtc attcatcagg acggcacacc cataattttc cccgaccaga tgccgtgcta
     7321 tctctgcgac gatacgcctt gtatcgccgc ctgcgcgacc gaagccctgt tgcctgtggg
     7381 cagtattcaa gaggcccgga tgggagtggc tgtggtcaac catcgtctct gcaccgccgg
     7441 ccaggggtgc catgcctgtg tgtcaaaatg ccccaccgat gcattgacca tggatttcga
     7501 tacccaacgg cttgtagtcg cagttgagcg ttgtgtagga tgcggaatgt gcgagcacgt
     7561 ttgtcgcacc gtgaacgatc acatcgccat tagggtcacg ccctttcgtc taatggaatc
     7621 gccccgctga ttaagtgtag aaagcagcaa ctttaggtcg caccagccgt atctctgagc
     7681 gccagaattg tcgggaaacg agtcacgtct gacagatgtt tcgacggatc agaatcgtgc
     7741 ttgacaagcc ctcaaccttt acctatcata cccaggcttt cgccaggcgt ttgcccgccg
     7801 ttacccaaga aatgtctggg tcaaccgggt cgaagcgagg agtattttct catgacgagt
     7861 aagcagtcgg tacaaggttc tacacagacc tcagccgccg tcctttccaa atctcccatc
     7921 aaagattccc ctgcagtcga acgcgccctc aatcgcagca agctctatct cttggtttcc
     7981 tggagcttgc tctaccctga agacgacgaa tttttggact atttgcagtg cggagagttc
     8041 gtggaagacg gccgcgccgc gttggaggcg ctggaaaccg cactcgacgg catcggcggc
     8101 gatcgggcga agcaaaaact ggctttaatg cgcaagcaat tcgatcagat cgaaaagctc
     8161 attgcgtccg aatgcgtgaa ctggcaattg agcgaattgc aggccgaaca ccggcgcgtg
     8221 ttcagcaacg tcatcacgct cgactgtcct ccctacgaaa ctctctttgg caacgatcac
     8281 gtcttcgcgc aatcccacgt catgggcgac attgccggat tctataaggc cttcggtgtc
     8341 gaactctcca aagacatcca cgaacggctc gaccacctga gcgtcgaatt cgagttcatg
     8401 catttcctgg cttacaaaga atcctattcc cgctgtcatg acggcgccga aaaaacccaa
     8461 atcgtggtcg atgcccaaaa gaaattcgtc aagaatcaca tcggtcgttg ggtgccgttg
     8521 ttctgccgca tgctgatcaa gaaggccgac tcgggtctct tcaagcacgt cgcggacatg
     8581 acctccgatt ggatggattt cgaagcggcg ttcctcggtg tcactcctca gacctattca
     8641 gaaactgatt accggccggc gacgttcaat tcacctgaag gccagaccta cgagtgtggc
     8701 gcgcaggatc aaggcaacga gctcaacctt ttattgaacg aaacaggcgc acagaatttc
     8761 atggatcaaa aggaaaagga caaggacaag gaggaggacg ggccggtcgg gaccgcctaa
     8821 cgatccgttc agcgtcggtg tttcctttta taccgtttga tgcagctatt ttgggtcagt
     8881 cttaatcatt taattgagag gagggcatag tccatgagag tagtgcagac acacaacaag
     8941 tctgtggtgt ttggcattct tctctctgcc ctgatcgttg gcgtgatgtt gacgattggg
     9001 caggtgccgt tggccgtcag ccaaccggtg accatcccgg cgaaggcggt caagggaccc
     9061 atcccgatgg atggagcgaa ccctgtgtgg gagagcgttc caggggtcat tgtgccattg
     9121 agcggccagt tgatcaccac cccgatgcat ccgaatatct cggtcaaatc ggtattcgtc
     9181 aaggccatga cgaatggcaa ggaagtcggg ttgcgcgtcg agtggagcga tcagaccaag
     9241 aatgacacga cgatcggtcc ccaggacttc cgtgaccagg ccgcaatcat gttcccggtc
     9301 aacacgtccg gtgcgccgcc atttcagtgc atgggtcaat ccggcgggac caccaacatt
     9361 tggcgctgga atgcagagtg gcagaaggac ctcggcaagg atagcgctgg actctgggac
     9421 gtagacgatc aataccccgg cattttctgg gactactatt ttgaagagcc tgccggcggc
     9481 gtgacctatc cagaccggat cggccgcagc cttgggccct tcaaccccgg catttggtcg
     9541 ggcaacatta tgtctgaccc gacactgcgt gtgagctcgg tcgaggatct gaatgccaac
     9601 ggcttcagca ccttgacgac ccaggctcac caggacgtga tcggcaacgg tgtgtgggaa
     9661 ccggccggtt ccgtcaaggg cggtggttat agcggcccga cttggcgcgt ggtcgtgaag
     9721 cggaccctgg agacaggcga tgctaacgac gtgcagttca agggtggcat gtccgtgccc
     9781 atcgcgtttg cggtgtggga cggcaacaac atcgagcgta atggtatgaa ggcgctctcc
     9841 acctggttca cgctcaagct tccgtgagct aacaggtctg agtgaccatg ttccaccttc
     9901 ccccggtgga acagacagcc aaagaaaagc cccagttctc ttgagctggg gcttttctat
     9961 ttatgggggc tgttgacgac cgggggtggg ttgcatttga gcgggggcgg agtgtataaa
    10021 acaaagtcct tatctttcga gatttatctt atttttcaat tctctcggta gagatttggc
    10081 atgaaagttt cactgctttt cccaccgacc tggcacccgt cgcagcccta tctgagtctt
    10141 ccttcgctta ctgggttttt ggctcaagcc ggcgtcaccg atgtgagtca acgcgacttg
    10201 ggcatcgagc tcctcgacga ggtcgtcacg aagtcctatg gcgccggtct ctatcaggat
    10261 ctcctggaaa aaaagcagac acttgagcga cagcgaaccg gcgataccgg tcccggcagc
    10321 gccgagcatc tggccagaat catcgagtca ctcgaccggt ttccctatct gatcgatcgg
    10381 atcgagctcg ccaaggagac gctccgtggc gagggcttct acgacatcga agcctatcgg
    10441 gaaagcctct ttttgatcga taagtggctg gaagtcattt ccactttgta ttttccgacc
    10501 agactgaccg tggtcgacaa tcaattcagt acctattcca tttactcgtc caaggatttg
    10561 atgaaagtca ttcgggacga gcagcagaat ccgtacatca aactgtttcg cgaacgcttt
    10621 ctgccttcca tcatcgccga gcgccctggc ctgatcgggg tctcgatcac ggcgacttca
    10681 cagatcattc caggcctgac gctctgccgg ctgatcaagg aggctgctcc cgacatccat
    10741 ttgacgattg gcggcagcat cttcacacgg ttggttgata atttgcgtcg gtgtcccaac
    10801 ctctttgata tcaccgacga catcgtcgta ttcgaagggg agacggcctt gctcgagctc
    10861 gtcaatcaga tggccgggaa aaaggatttt agcaaggtgc cgaatctgat ttatcgccag
    10921 aacggcaaga tcaccgtcaa tcagccattc tattccgaga acgtcaacca actgccggca
    10981 cccaattacg acggctttcc gctggaccgc tatctctcgc ccgagccggt cctgccggtg
    11041 cagttttccc ggggttgcta ctacaaggac tgcgcgttct gcgccttgac gctcgatcac
    11101 cagaatttcc ggcaaaaaga tccgggccgg acgatcgagg agctggaatg gctcaaacag
    11161 cgttacggcg tgcggaactt cttctttacg gatgaatgct ttgccctgtc gccgacgaaa
    11221 cgcctctgcc agcaaatgat cgagcgccgg gtggacgtga aatggacctg cgaaatgcgt
    11281 ttcgagaagc atctcacgcg cgagttgctg acgtcgatgc gagacgccgg atgtttgaaa
    11341 atcgtcttcg gcctcgagtc gttcaatcag cggatcatgg atttcatgaa gaaaggcatt
    11401 cagcaggagt gggtccgacg tatcgcgagc gattgcgtgg atctcggcat cgctgttcac
    11461 tgctacatca ttgtcggctt cccgacggaa aaggaagagg aagcgctgga gacgatgaat
    11521 ttcatcgtcg agaataaaaa gctgaacgag tcctacggct tctcctgcca gccctgcctg
    11581 tttgatctgg aaaaggaagc tccgatcatg agcgatccgg gaggctacgg catcagacgg
    11641 atcatgcggc ccgcggccga agatttgagc cttggatttt tctatgaagt gcaggaaggg
    11701 atgacgcccg atcaagcaga gcggctctat cagcatgtgt acgaaaaaat cagtgaggtc
    11761 gtgtgcgagc tgccgttcaa ctactcgatg gcggacgggc ttctttacat cgcccgggcc
    11821 aaagcggaag aacggcgcgc ggtgaccgcc gcgcagacat agcagacaca agggtgagga
    11881 gacaggcgtg cccgcaactg caacaaccat cgagcgcatc accggaaagc tgagcgacca
    11941 gccgctcctg ttccaataca cgatcaagct tgtgctgttg gtcgcgtttc tggaactgat
    12001 cctgtaccgc ctggtttccc ggttgggcat gcacctgagc aagctggctg ccgatcatca
    12061 gtggatcatt ccaaccttta cggccctcac ggaagtcggc cagtggctct tgaatgtggt
    12121 cgcaattctc ttgttcctcg gcttgacggt ggggatggtt aaccgcgtgg cccagcgagg
    12181 ctttacgggt ttaacccggc tcgtcgtgcc ctgtatcgcc ttactgttgc tgctgaccgt
    12241 cggctttcta ttggtgccgc cgtcaatgct cgggtcggcg atctataaca ccgtcgccct
    12301 ggcggcgatc gtgttgctga tgagcgagta cctggcgacg catcacgatc gttcgcaccg
    12361 tgtgctcggc ctcacgtacc tcttgggcat ttccggctgg ctgtactatc agatcgtgtc
    12421 gacgttcttc agtttttcag gcagcgtggc ggcgccgccg atggtgtacg aatcgcaccg
    12481 cgcgggtgaa gcattcatgg tgctcgcgag cattcttgtg ttttgggcct atgggagagg
    12541 cgtttcgttc cggtcgcgca atcagcgcca gcggcaccgg gccatttggt tctggagcac
    12601 ggcggcggcc attttcctga cgatgttttt tctggattat ctgctcaacc agtataatcc
    12661 ggggctcgcg agcaatgtgc gcaaggccgc cgacggcatc ggatggattt ttcagttcgg
    12721 gatgggctac accttttatc tcccattcgc gctctatatg gccggattgc tctgctggtc
    12781 ctacacggtg atcaagttgt tgaccatggg gcggctggcc ggctacggcc tggggctgat
    12841 gtttatcgcc gggtatgcgc tgttgtattc caatctgaca ctgatggtcg tgctgggcgt
    12901 gatgctgatg acgatggatc gctaccgacg tgacgccgcg gactcggtcg cggatcgggc
    12961 ggtactacga acgaccgact cgttggtcgg cggacatgtc taatcttttg ccataggggc
    13021 ttgccatgga agaaacgacg tcttcggagc agagtcgggc ggcggtggat ccgggcgatc
    13081 agccgttgaa cccggatgag gaaattgccg aaattgagaa gctcttggcc gccgagccgg
    13141 atgattttca agcccggtgc cggctgggtg aactctattt cagcaagggc cggttggacg
    13201 acgccttggt cgaggtcaag aaatcaatcg agatggccga gggcattcgc gcagaaatga
    13261 atcgttcgct ggcaatgtac tactcgaatc tcggcacgat ttacgcgacg aagaacatgg
    13321 cggacgaggc ggaagccgag ttcaagcgcg cgttggatgt gtttcaatac gacgtgctgg
    13381 cgcttttcaa tttggggcgt ctctgtgccg acaagaaaaa gtacatggat gcgaaggggt
    13441 tttatgagcg gctcgtcgaa atcacgccgg acgatccgat ggcctggtac aatctcgccg
    13501 gcgtctacgt ggaactcgac aacccgcagg tgtcggacta caacacgatc gacgccgcga
    13561 ttcaatgcta tctccggacg ctcgaattgg accccaagca tctggaagcg agcttcaagc
    13621 tgatggagct ggctctcaat cacaagaaat ccgatctcgc catcaaagtg atggaagacg
    13681 ccgtcgaaca caatgcggac gagccgctgg cctactacaa cctgatcagc gtctacgaca
    13741 agtgcaagat gttcgagcag gccgagcagg cccggcagcg gttgaaagaa cgatttgcga
    13801 agcggagtaa aacgagcgca tcctgattca cccttatcgc ggaggaacac accatgtttg
    13861 ggagtatggg tatcacagag ctgattctga tcctcttcat cgtgctggtt atttttggtg
    13921 ccggcaaact gccgcaattg ggagaaggac tcggaaaagc catcaaggga ttcaagaagt
    13981 cggtgcatga agcggatgcc atcgaagccg aagctgaggc cgcagcggcc cagcagcaaa
    14041 tggcgtcgcc tcagccgtcc atttcgacgg cgccggcccc cggcgtggaa caaccggccg
    14101 cgcaatccgc gactcgcgca tagcgcgacg atcggccgaa cgccatggac acagaacttc
    14161 agctcgccgt cggctacatc gagacct
//
LOCUS       DASTQY010000202         5357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_302349, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000202 DASTQY010000000
VERSION     DASTQY010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302349"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="VFR82_06795"
     CDS             complement(<1..1280)
                     /locus_tag="VFR82_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501343.1"
                     /translation="MRFTYIIGFVSTVLLASAAPSWGTESAVAPGKNTSNTVPLYTNL
                     GSHHKSISTQVPATQQYFDQGLRFIYGFNHAEAIRSFTRAAELDPTCAMCFWGIALAY
                     GPNVNAPMDSASGVAAYAAAQKAITLKSHATGPERAYIEAVAQRYEAVPTTDRVRLDT
                     LYSRAMERVVNTYPDDLDAATLYAESLMDLRPWNYWQPDGSPYPGTNEIVRQLQRAIS
                     RNPNHPGACHYYIHAVEAVNPEAAMPCAERLARLMPGEGHMVHMPAHIYIRVGRWNDA
                     VQANQHAIHTDEVFIEGQHPKGVYPLAYYPHNIHFLAFASTMAGRGTQAIEASNTLTS
                     KVNLDAARQVGMLQEMLPYHALTLTTFGKWDEVLVEPLPPADIPFSHAMAQYARGVAH
                     AAKGQWTEARAALDTVTAINATTLEGAEGKTALS"
     gene            complement(1630..2187)
                     /locus_tag="VFR82_06800"
     CDS             complement(1630..2187)
                     /locus_tag="VFR82_06800"
                     /inference="COORDINATES: protein motif:HMM:NF019819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEU4501344.1"
                     /translation="MKTLQSLGIGSVFLAVAACGGMAQREPLTTVPSVDLSRYSGTWH
                     EIARLPMWFQRHCVDSKAIYTSRPDGTIGVHNECETDTGGLDQVEGVATVVDQKTNAR
                     LTVVFDNFFARLFGSSRQGNYWILDLDPDYRMAMVGTPDRRYLWILSRTPRLDEPTYE
                     RLVAKAQGLGFPVRDLIRTRRSSLP"
     gene            complement(2540..3463)
                     /locus_tag="VFR82_06805"
     CDS             complement(2540..3463)
                     /locus_tag="VFR82_06805"
                     /inference="COORDINATES: protein motif:HMM:NF021640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HEU4501345.1"
                     /translation="MKEIFIGSSKEGLEQAMQVAAALSQLKDVQPLLWTDVFKLGDIT
                     FMGIENIASRVAGAVFLATPDDDSVIRERRVRTPRANVLFEYGYLTAMLTRRRVALCL
                     YSSVELPSDFAGLTYVPMGNFEPTKPLDHQAIVKLKSWATELPTVQIGFSPTCQLHGY
                     SGLWQTEIVYQVWRRIQFKDPDYAALSGKTILQIPPDGDSGTGCFFGNFQVQIGSCYA
                     EFEISDRVIDVKVLGDGSMKIRNGLQSRQRIRLEGDPPQRDGFEPDLRGAREIDLFIH
                     CSTDEPGVLRGHLSSEVGGNIYSKATGKWYR"
     gene            3723..4268
                     /locus_tag="VFR82_06810"
     CDS             3723..4268
                     /locus_tag="VFR82_06810"
                     /inference="COORDINATES: protein motif:HMM:NF018580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HEU4501346.1"
                     /translation="MIKAHSVLSFCFILAMAAGLSQAQEAPPASPAAPETAAKATPAD
                     GHVIHVTAPHLIDGKVRGPFHHYCKIVSPEPFIECLLYETEDNNAKLIGVEYIVAKTL
                     TRDEKVLPKKAWKKVWHDHSVEGVIGNIKMLDMAPDKAKEFADTVAKTDGIIFSLWPE
                     GAKLPTGKTSIGMMVGHPIHQ"
     gene            complement(4578..4928)
                     /locus_tag="VFR82_06815"
     CDS             complement(4578..4928)
                     /locus_tag="VFR82_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4501347.1"
                     /translation="MKVKRIVANVKAKNIGDAKRFYEKVLGLDELMNLGWIATYGSTT
                     QSIVQMSFMKEGGSGAAVPDISIEVDKVDEAYKRMKEEKFTIEYDITDEPWGVRRFFV
                     RDPFGKLVNILSHM"
     gene            complement(5118..>5357)
                     /locus_tag="VFR82_06820"
     CDS             complement(5118..>5357)
                     /locus_tag="VFR82_06820"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEU4501348.1"
                     /translation="TWNYNQNYNHSPPLRGGNPMGKIIECAKVDPSSGCKHVVRGNTE
                     EEVLKNAMEHAKQHGIREVTPELKAKVKAAIRDEK"
BASE COUNT         1167 a         1398 c         1502 g         1290 t
ORIGIN      
        1 atcgagagcg ccgtcttgcc ctcggcacct tcgagcgtcg tggcgttgat cgctgtcact
       61 gtgtcgagtg cggctcgcgc ctccgtccac tgtcccttgg cggcgtgtgc cacaccgcgg
      121 gcgtattgcg ccatggcatg cgagaaggga atgtcagccg gcggaagcgg ttcgacaagc
      181 acttcatccc attttccgaa cgtcgtgagc gtgagcgcgt ggtacggcag catctcctgc
      241 agcatcccga cctggcgcgc ggcgtcgagg ttcaccttgg aggtgagtgt attggacgcc
      301 tcgatcgctt gggtaccgcg gcccgccatc gtggaggcaa aggcgaggaa gtggatgttg
      361 tgcgggtagt acgccagtgg atagacgccc ttcgggtgtt gcccttcaat gaacacctcg
      421 tcggtgtgga tggcatgttg gttggcctgt acggcgtcgt tccaccgtcc aacgcggata
      481 taaatatgtg cgggcatgtg gaccatgtgt ccttcacccg gcatgagccg agcgagccgc
      541 tcggcgcatg gcatcgcggc ctccgggttt accgcttcga ccgcgtggat gtagtaatgg
      601 catgcgccgg ggtggttcgg gttgcgcgag atggctctct ggagttggcg tacgatttca
      661 ttggtgccag ggtagggact accatcgggt tgccagtagt tccacgggcg gaggtccatc
      721 agcgattcgg catacaaggt cgccgcgtcg aggtcgtcag gatacgtgtt gaccacccgc
      781 tccattgcgc gcgagtacaa tgtgtccaaa cggacacgat cggtcgtcgg aactgcttcg
      841 taacgttgcg caaccgcctc aatgtaggct cgctcaggtc ctgtggcgtg ggatttgagc
      901 gtgatcgctt tctgggccgc cgcgtaagca gcaactccgc ttgccgagtc cattggagcg
      961 ttgacgttcg ggccgtaggc aagcgcgatt ccccagaagc acatggcgca ggtgggatcg
     1021 agttctgcgg cgcgcgtgaa cgaccgaatc gcttctgcgt gattgaatcc gtagatgaat
     1081 cgcaagccct ggtcgaagta ttgctgtgtg gctgggactt gagtggagat ggatttgtga
     1141 tgggagccga ggtttgtgta gagggggact gtgttagaag tgttcttccc gggcgccacc
     1201 gccgactctg tgccccaact aggggcggca ctggcgagca aaacagtcga gacgaatccg
     1261 atgatgtagg taaacctcat gacgtgcctc cttccgaacg cggaatgcaa ttggtttgac
     1321 cgttgggtca aagtagtgag gtgcattaac ccatagcaga gtgatctgga gatgaacgtt
     1381 gaaattcgat taggacgagt ctttagagtt tccacgtcgg atgataatac gctggtgccc
     1441 atatctgttc tcctttatat cgtgacggct ctcgcttcaa atcatggctc gcactataag
     1501 ggctatttgt atggatgtct tatccgggag tctgattctc ttaacagagg cgtgctaggc
     1561 tgcgtgtaat tgcttcggcg tccggattcg tttgccgaaa cgccgatcct gagagtggag
     1621 cgaaacggat tatggaagcg aagatcgcct cgtccgaatc aggtccctta cgggaaagcc
     1681 tagcccttga gcctttgcaa ccagacgctc gtatgtgggc tcgtcaaggc ggggtgtgcg
     1741 agacagaatc cagagatacc ggcgatcggg agtgcccacc atcgccatgc gatagtccgg
     1801 atcaagatcg agtatccagt agtttccttg acgtgaggaa ccgaagagcc gcgcgaagaa
     1861 attgtcgaag accacggtga gccgggcatt cgtcttctga tcgactacag tcgcaacgcc
     1921 ctccacttgg tcaagtcctc cggtatcggt ctcacattcg ttgtgcacgc cgatcgtgcc
     1981 gtcgggacga ctcgtgtaga tcgcctttga atccacacag tgccgctgaa accacatggg
     2041 cagccgcgca atctcatgcc aggtaccgga atatcgggag agatcgaccg agggaaccgt
     2101 tgtgagcggt tctcgctgcg ccatgccgcc acaagcagcg accgcaagaa agacgctacc
     2161 tatcccgagt gattggagag tcttcatgaa ggatttccga gtgtcttttt tccaggcgat
     2221 gacataggag atatggcttc aaccggcacg agccggcccg tcaagcgccg caaaactcag
     2281 aatgtctcca tttcaattat ttcatagcat ctccccagtt tcttcaaaat gctttaactc
     2341 ttgaatactc ttcttaaaga gcggcctcca gtcgggttgt gaggcatagt gggagggctc
     2401 gctaagggac atgacataga caactgctgt tttcttagcc cattgcttgg cgtgctccag
     2461 cccatccctt gatatcagag tttcaaggcg aaggcgttcg ctcatcctca aagcctagct
     2521 gtacatccat acgcgagcat tacctgtacc attttccggt tgccttggag taaatattgc
     2581 cgccgacctc acttgaaagg tgtccacgga ggacgccagg ctcatcggtt gaacagtgaa
     2641 tgaacaaatc gatctcccgc gcgcctcgca aatcgggctc gaaaccgtcc ctctgtggag
     2701 ggtcaccttc caatctgatg cgctggcgac tctgcagccc gttgcggatt ttcatgcttc
     2761 catcccccaa aactttcaca tcgatcactc ggtcgctgat ctcaaactca gcgtagcaac
     2821 taccaatctg gacttgaaag ttgccgaaaa agcagccggt gccactatcg ccatcgggtg
     2881 gaatctgcag aatcgtcttt ccactgagcg cggcgtaatc tgggtctttg aattggatac
     2941 ggcgccacac ttggtacacg atctccgttt gccataaccc tgaataacca tgcagttgac
     3001 acgtcggcga aaacccaatt tggacagtag ggagctcagt ggcccatgat ttgagcttca
     3061 caatcgcttg atgatcgagg ggcttggttg gctcaaaatt acccatagga acgtaggtca
     3121 gccccgcgaa gtccgatgga agttcaacgc tggagtaaag gcaaagagca actctcctcc
     3181 gagtcagcat ggcagtgaga tacccatatt caaataagac gttcgcccgg ggcgtcctaa
     3241 cgcgcctctc gcgaatgacg gaatcatcgt ccggtgtcgc aaggaaaaca gcaccggcga
     3301 ctcgactcgc aatgttttcg atgcccatga aggtgatgtc gccgagcttg aagacgtctg
     3361 tccatagcaa aggctgtacg tctttgagct gcgagagagc ggccgccacc tgcatggctt
     3421 gctcaagccc ttctttgctg cttccaataa agatctcctt caatgtggct cctttaggaa
     3481 gtcgcgactt gcctgccaag actttctcga gactttctac cgagaaacag tcgcatctta
     3541 cctggctgcc tttcggcaaa gaactgtcaa atgtaacagg gcgtaagcgt ggatttttaa
     3601 ataggggagg gtgggctcgc cgtgcaccgc gaaagggttg acggatgcca tttcttcagg
     3661 cctttcctca cttatcttgt tttcgtaagc tataggaact tgtcttagaa aaggaggccc
     3721 ccatgataaa ggcacatagc gttctaagtt tttgtttcat ccttgccatg gcggcagggc
     3781 tttcacaagc tcaagaggca cctcctgcct cgcccgctgc accggagact gccgcgaaag
     3841 cgactcccgc agacggccat gtcatccacg ttacggcacc gcacttgatc gatggaaagg
     3901 tgcggggacc tttccatcac tattgtaaga tcgtttctcc tgagccgttc attgagtgcc
     3961 ttctctacga gacggaagac aacaacgcta aactcatcgg tgtggagtac attgtcgcca
     4021 agactcttac tcgcgacgag aaggtccttc cgaagaaggc ttggaaaaag gtttggcatg
     4081 atcattccgt ggaaggagtc atagggaaca tcaagatgct ggatatggct cccgataagg
     4141 ccaaggagtt tgcggacacg gttgccaaga ctgacgggat catcttttca ctatggcccg
     4201 agggggccaa gttgccgacc gggaaaacta gcattggcat gatggttggg catcccatac
     4261 accaatgaca tggaaccata atacctcccc acgtctcggg tcgaccgttt gttcagtcgt
     4321 ctgggacagg ctactcccga acgatcgcgc atgatgttcg ccgttcggaa agaggagagt
     4381 gaaggagcgg taaaatcgcc gataagagtc agcggatttg tcctgtccgt ctgggatggg
     4441 tgacgtagac ggagattcga gtctgacttc ggccgaacaa accaggacat tttactttgt
     4501 cacgtggggg gtctctcacg gtgtgaggtg cgatgagcac tgagggtgtg aggcgaggga
     4561 ggattctcgt tctctactta catgtgagaa agaatattaa ctagctttcc aaatgggtct
     4621 cgaacaaaaa aacgacgaac tccccaaggc tcatcagtta tgtcgtactc gattgtaaac
     4681 ttttcctctt tcattcgctt gtaggcttcg tcaactttat cgacttcgat agaaatgtca
     4741 ggaactgccg ctcctgatcc tccctctttc ataaagctca tctgcacaat tgattgtgtt
     4801 gtggaaccgt aggtagcaat ccacccgaga ttcattaatt catctaagcc taaaactttt
     4861 tcataaaagc gttttgcatc gccaatgttt ttggctttga cattggccac tattcgtttc
     4921 accttcatgg gtttcctttt atgaaatgga ccaagaaacc agaacattct acgttttcac
     4981 ggggacgtgg tctatcgcgg tgtgaggggc ggtaagcgat gagggaacgt gaacgcgagg
     5041 aacgagccgg cccgtcccca atcctccggt agcgaggcgg gccggatcaa gctttgataa
     5101 agagcctgaa ggatcagtta cttctcgtct cgaattgctg cctttacttt ggccttgagt
     5161 tccggggtga cctcccgaat cccatgctgc ttggcgtgtt ccatggcgtt cttcaggact
     5221 tcctcttcag tgttcccgcg aacgacatgc ttgcatccgg acgacggatc aaccttggca
     5281 cactcgatga ttttacccat tgggtttcct cctcttaggg gcggggagtg gttgtagttc
     5341 tggttgtagt tccaagt
//
LOCUS       DASTQY010000203         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_974252, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000203 DASTQY010000000
VERSION     DASTQY010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974252"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2493
                     /locus_tag="VFR82_06825"
     CDS             <1..2493
                     /locus_tag="VFR82_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4501349.1"
                     /translation="KGDRIQNFETVRRRKDGRLIDIDLTVSPVRDGNGTIIGASKIAR
                     DITERKQTKQAITEAQVRLATTLESIGDAVLATDTESRISYLNPVAERLLGCSIGEII
                     GQPLDKFFTIVNAETRQAVPSPVERVLREGTVAGLANHTVLLRRDGTEVPIDDIAAPI
                     KDADGRLLGVVLVFRDVSDRYEAARQAVLLSSIVASSDDAIVSKDLAGTITSWNRGAE
                     RMFGYTAEEMIGSSILRIIPTDRHSEEVAIVSKLRRGERVDHYETIRCKKDGRLFDVS
                     ITISPLTDDDGNVIGASKIARDISEQKRTQRALHESEERWRVTLESIGDAVVATDASA
                     VVTFVNPIAVDLLGMPAAEIMGRRLAEVFTIVNEETRQAVDNPVERVLRDGLVVGLAN
                     HTVLVRPDGTEVPIDDSAAPIRDHDGRLIGAVLVFRDISQRKSSELQVQRWNAELEAR
                     VRERTGELVNSQERLRALASQLNLTEQRERRRLAGDLHDYLAQLLALIRIKLGQALQR
                     LNDRAPEVRSALTEMDALLQQSLQYARTLMAQLSPSVLQDLGLIPAIHWLAEQMVQQG
                     LTVDVRILTDEQPGFSEHQSDLLFQALRELLTNVVKHAGVSSAVVTVNKQDRDTWLIT
                     VQDTGAGFDLNAVQYLPSGEHFGLFSIQERMEAMSGWCRIHSLPGEGTTVELGLAAMP
                     ADASRKPHVSGTAVGVADAPRAAVPSRWRVLLVDDHAMVRQGLRSILETYSDLEIIGE
                     AADGFEAVESAMSTQPDVVVMDINLPKLNGIDATKQIKNHAPNAVVIGLSVQHSAQTL
                     NAVLDAGASALLSKEQATVDLYRTITHFLKVR"
BASE COUNT          568 a          729 c          816 g          532 t
ORIGIN      
        1 aagggcgatc gcatacagaa tttcgagacc gtgcggcggc gaaaggatgg ccgtctcatt
       61 gatatcgatt tgacggtgtc tcctgtgcgc gacggcaacg ggaccatcat cggagcctcc
      121 aaaatcgccc gcgacattac cgagcggaag cagacgaaac aggccatcac tgaagcgcag
      181 gtgcggctgg caacgaccct ggaaagcatc ggagatgccg tgctggccac cgacaccgaa
      241 tcccgaatct cctatctcaa tcctgtggcg gagcgattgc tgggctgttc catcggggaa
      301 atcatagggc agccgcttga taagtttttc accatagtga atgcagagac gcgacaagcg
      361 gttccctctc ctgtcgagcg ggtcctgcgc gaaggtacgg ttgccggctt ggccaatcac
      421 acggtcctgt tgcggcggga tggaacggaa gttcccatcg atgacattgc ggccccaatt
      481 aaggatgcag atggccggct gctcggcgtg gtgctcgtgt tccgagacgt gtcggaccga
      541 tatgaggctg cgcggcaggc ggtactgctt tcctcgatcg tggcttcctc agacgacgca
      601 atcgtgagca aagatctcgc aggcacgatc acgagttgga accgaggcgc cgagcgcatg
      661 ttcggctata ccgccgaaga aatgatcgga tcctcaattc tgcgcattat tccgacggac
      721 cgtcattcag aagaagtcgc gatcgtgtca aagcttcggc gcggcgaacg cgtggaccat
      781 tatgaaacga tcagatgcaa aaaagatgga cgacttttcg atgtgtcgat cacgatctcg
      841 cctttgaccg atgacgacgg caacgtcatc ggtgcctcaa aaatcgctcg cgatatctcg
      901 gaacagaaac gcacccaacg ggcgcttcat gaaagcgaag agcgttggcg ggtgacactg
      961 gagagcatcg gcgatgctgt cgtcgccacc gacgcctccg cggtcgtcac ctttgttaac
     1021 ccgatcgctg tcgacctgtt gggcatgccc gctgcggaga tcatgggacg ccgattggca
     1081 gaggtgttca ctattgtgaa tgaagagacc cggcaggcgg tagacaaccc tgttgaacgg
     1141 gtcctacggg atggcctggt cgtgggcttg gcaaaccaca ctgtgctcgt gcgccctgat
     1201 ggcacagaag tccccatcga cgacagcgct gcacccatcc gggatcatga cggacggtta
     1261 attggtgccg tgctcgtctt cagagacatc tcgcagcgca agagttcgga actgcaggtg
     1321 cagcgttgga acgctgagct tgaagccagg gtgcgggagc ggacgggaga gctcgtcaat
     1381 tcccaagagc gacttcgtgc gctggcatca cagctgaatc tgacggagca acgggaacgc
     1441 cgccggttgg ccggcgacct gcatgattat ctggcgcaac tcttggcctt aattcgaatt
     1501 aagttgggtc aggctctcca acgtctcaac gacagggcgc cggaggtccg gtctgcgctc
     1561 accgagatgg acgcactgtt gcagcagtcc ctacaatatg cgcggacgct gatggctcag
     1621 ctcagcccgt cggtcttgca ggacctgggg cttatcccgg ccattcattg gttggccgaa
     1681 cagatggtgc agcaagggct cacggtggat gtccgcattc tcaccgatga gcagccgggt
     1741 ttcagcgaac atcagtccga tttgctgttc caagccctac gggaattgct caccaacgtt
     1801 gtgaagcacg caggcgtttc ctcggccgtg gtgacggtaa acaagcaaga tcgggatacc
     1861 tggttgatta cggtgcagga cacaggcgcg ggtttcgatc tcaatgccgt gcagtacctg
     1921 ccctcgggtg aacatttcgg tcttttcagc attcaggaac gcatggaggc gatgtccgga
     1981 tggtgccgga ttcactcgct gcctggcgag gggaccaccg ttgaactggg gttggcggcg
     2041 atgccggcag atgccagtcg gaaaccgcat gtttccggaa ccgctgtggg agttgccgat
     2101 gctcccagag ccgccgtgcc gtcgcgttgg cgtgtgttat tggtggatga tcatgccatg
     2161 gtccggcagg gtctgcgaag cattctggag acatattccg acttggaaat catcggggag
     2221 gcggccgacg gttttgaggc ggtggaatcg gcgatgagca cacagccgga cgttgtcgtc
     2281 atggacatca accttccgaa actcaacggg atcgatgcga cgaagcaaat caagaatcat
     2341 gcgccgaatg ctgtggtgat aggattgtcc gtccaacatt cagcccaaac cctcaatgcc
     2401 gtgctggatg cgggcgcttc ggccctgctg agcaaagagc aagcgaccgt ggacctgtat
     2461 cgaaccatca cgcatttcct caaggttcga tagaacatca cgctgccaac ctatggatct
     2521 tagtgccgct accagcatag agatagatgg acctgccggg cggagttggc accgccatcc
     2581 gctggcgttg aagccccgca ctcccagacg gagagccgta gctgttacgg ggcaatcatg
     2641 aagtc
//
LOCUS       DASTQY010000204         4224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_901390, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000204 DASTQY010000000
VERSION     DASTQY010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901390"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VFR82_06830"
     CDS             complement(<1..911)
                     /locus_tag="VFR82_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501350.1"
                     /translation="MWIRAVLCALFCLLTACGGSDSSSETPPPGQQPPPPSGPAGPWP
                     LADLTVYGSAQSLTGTIIDASPDDAQNIWAVTPDALYVLRPGQTTFRRFTAADGLHVQ
                     TYTDPSNGQQTVSNITAMAGGIANEVFVGYRGYEAPIPPPAPPPCCVANADFSDPRWH
                     LGQADKVTLDPGGGIQVRRYEFLCDITVNCWEERSARRMIFGHSGTAAGHLFIGFDHG
                     VAHVFNDVFGDHTHIVTFWHTADGRNVMRMGEHYGLALYPNGEMIDGTGYGIGSHEWN
                     ADPRAWVNGRFVWGFTTYGPAEPYNNG"
     gene            1072..1716
                     /locus_tag="VFR82_06835"
     CDS             1072..1716
                     /locus_tag="VFR82_06835"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEU4501351.1"
                     /translation="MKPLTMSVFVAMFACSVAAYTVRAAEPSFELTARYILEVVKAFR
                     TAYVLKVVEHARDGGVKPHENWQSDAHFIPLPAQFVKAAADQLDNFEIGLIGLTPVNQ
                     ANYPRTQAESDALIQLMKNRERSVTTFVDGDQFKAISADLALVQSCVDCHNHHPKATR
                     RDFQRWDVMGGLVVRLKRDARPEGSPIGPEPPGRPSGPIERLTPPLTAPPPWVR"
     gene            2024..2974
                     /locus_tag="VFR82_06840"
     CDS             2024..2974
                     /locus_tag="VFR82_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501352.1"
                     /translation="MSYRYSSVDIIVGVGVCAIVFGALLMFVATTGTFLVGNPFTAMA
                     QGSLATTESAWLQPALGQAIVERALLQIQTDRITASAASEWRQALQSHRSLQIVIDNP
                     FAMVISRAKNIPSEHEARVQAVMGRSIVNFTQRGIRSGVFSADLYLSDYNQRMIGATE
                     QRGKRMHDGFASTWQSVLGKGIVDASQEHLQRAGVVQEQLGSAILHVAQARMFLDDAW
                     SSNQYQLGSLMAAFDRTGGMNEPVTRPVSAVAGQVEVATSSVGMPGIPDIPMSYLMAA
                     ALALFSIFFGGLILSAAGREAKALTEARRNTGRWVYRMAS"
     gene            3245..3640
                     /locus_tag="VFR82_06845"
     CDS             3245..3640
                     /locus_tag="VFR82_06845"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4501353.1"
                     /translation="MQGHPVGLDGRRPSALNRFRRHPRVRINTPFSCALSPVRARGWL
                     RKPIINVGLVYDLSVRGARVSTEAPFKPGDQVALSLRLPRQIRSAEIAVATVRWTKDQ
                     FYGLAFRRLSLDAYDRLKKFVAIASRAVA"
     gene            3645..4022
                     /locus_tag="VFR82_06850"
     CDS             3645..4022
                     /locus_tag="VFR82_06850"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4501354.1"
                     /translation="MAQLERQPQPSVELRRYPRHRVSAPFPCAFSLLGLKRWTAVDRN
                     GLGVVLDVSLGGAKVMSTAAMNPGDHLAVSFRMPDQASALQLDANVKWVNDQLFGLEF
                     LSVPLSAESRLKKFLSRSPHTCS"
BASE COUNT          844 a         1234 c         1181 g          965 t
ORIGIN      
        1 tctccattgt tatacggctc ggctggccca taggtggtga agccccaaac aaaccgaccg
       61 ttaacccagg ctctcggatc agcattccac tcatgtgaac cgatgccata tccggtgccg
      121 tcaatcatct caccattcgg atacagagcc aggccatagt gctcacccat tcgcataacg
      181 tttcttccgt cggcagtatg ccagaatgtc actatatgcg tgtgatcccc gaacacgtcg
      241 ttgaagacgt gcgcaacccc atgatcgaat ccgatgaaca aatgtccagc tgcggtgcca
      301 ctatgcccaa agatcatccg ccgtgcagag cgttcttccc agcaattaac ggtaatatcg
      361 cagagaaact cataccgacg aacttgaatc cccccgccgg gatcgagcgt caccttgtcc
      421 gcctgaccta agtgccaacg aggatcgctg aaatccgcat tcgcgacaca gcagggtggt
      481 ggcgccggcg gtggaatagg tgcttcgtat cctctgtagc cgacgaacac ttcattggcg
      541 attccgcccg ccatcgcagt gatgttgctc accgtttgct ggccattgga cggatcggta
      601 taggtttgga cgtggaggcc gtcagccgcg gtaaaccgcc tgaacgttgt ttgtcccggt
      661 cgtaggacat acagggcatc cggcgtcacc gcccaaatat tctgtgcatc gtcaggactt
      721 gcgtcgatga tcgtgccggt taggctttga gccgaaccgt acaccgtgag atctgccagg
      781 ggccacgggc cagctgggcc ggaaggcggc ggaggctgct gtcctggcgg tggtgtttcg
      841 gatgagctat ctgaaccgcc gcatgcggtc agcagacaga aaagagcaca gaggactgcc
      901 cggatccaca tgtgagctcc tttgatttat tccttatcaa tgtctctact aaaagtccta
      961 ccgctgttcc gagattcccc tagctaggaa agacccctgc agaggcggtt atgacccctt
     1021 gaccggctgc gtgggcgcag gctactctcc cattttttta gaggagctga catgaagccg
     1081 ctcacgatgt ccgtgttcgt tgcgatgttt gcatgctccg tggccgccta cacagtgagg
     1141 gcagccgagc cttcgtttga acttactgct cgatacatat tagaggtcgt caaagcgttc
     1201 cggaccgcct acgtgctgaa ggtcgttgag catgcaaggg acgggggtgt gaaaccgcat
     1261 gaaaactggc agagcgatgc gcatttcatt ccgctgcctg ctcaatttgt caaagccgct
     1321 gcagaccagc tcgacaattt tgagatcggc ctgattggcc tgacgcccgt caatcaagcg
     1381 aattatccac ggactcaagc ggagagcgat gccttgattc agctcatgaa gaaccgtgag
     1441 cggtccgtca cgacgttcgt cgatggagat cagttcaagg cgatatcagc ggatcttgct
     1501 ctcgtgcagt cctgtgtgga ttgccacaac caccacccca aagccaccag acgtgatttc
     1561 caacgatggg acgttatggg tggtctcgtc gtacggttga agcgggatgc ccgtccggag
     1621 ggatcaccga tcggaccgga gcctccgggc cgtccctctg gtccgattga gagactgacg
     1681 cctccgctca ctgctccgcc accgtgggtt cggtgagtcg ccccttgacg ctccgtgcgc
     1741 ccggggcgta ggctttgaat agtttttttg ctgtcacata ccatttgggt acccccaatg
     1801 gtcctccgtg ggattcagaa ccgacggcct ggacctgccc ggtgcttctc accagacggg
     1861 atcgctctca tcttattgcc ttccgcacac cacggatcgc cgttgctgcg cgatcttttg
     1921 gctcaccttg agcacatcac agtttcacca tgtatgaccg agtcggccag gtatccctca
     1981 tgccggaggc tgaggtcttg cccgacgcgg aaaggaggat ctcatgtcct accggtattc
     2041 gagcgtcgat atcattgtag gcgttggtgt gtgcgccata gtcttcgggg cattgttgat
     2101 gtttgtcgcg accacgggaa catttcttgt gggcaatccg ttcacggcga tggcccaagg
     2161 atctctcgcg accactgagt cggcatggct tcagcctgct ttaggacaag ccattgtcga
     2221 gcgggcgctg ctccaaatac agactgatcg cattacggct tcggccgcgt ccgaatggag
     2281 acaggccctg cagtcgcatc gcagcctgca aatcgtcatc gataatccgt ttgccatggt
     2341 catcagccgc gcgaagaaca ttccaagcga gcatgaggcg cgagtgcagg ccgtaatggg
     2401 acgctcgatc gtgaatttca cacagcgggg cattcgaagc ggcgtcttct cggcggattt
     2461 gtatctctcg gattacaacc agaggatgat cggcgccaca gaacaacgag ggaaacggat
     2521 gcacgatggc tttgcctcga cctggcaatc cgtactcggc aaagggattg tggacgccag
     2581 tcaggaacac cttcaacgcg ccggggtagt tcaggagcaa ttggggtcgg ccattctaca
     2641 tgtggctcaa gcgagaatgt ttttagatga cgcgtggtcg tccaatcaat atcaactcgg
     2701 cagtctcatg gccgcattcg atcgcaccgg cggcatgaac gagcctgtca cgcgaccagt
     2761 ttctgctgtg gctgggcagg tggaagttgc gacgagttca gtcggcatgc cgggcattcc
     2821 cgacattcca atgagctacc tgatggcagc cgccctcgcg ctgttcagca tcttcttcgg
     2881 agggctcatt ctttctgccg ccggccgtga ggccaaggcc ttgactgagg caagacgaaa
     2941 taccggcagg tgggtgtatc gcatggcgtc gtgacgcact gcgatgttgg aaccactgga
     3001 ccacaggcag gcctgtggtc cagtgtctcg agcaagcact cgtttctgcc cctattgtct
     3061 ccccaagacc aacagcctcc cacaccccat ttgttccggt gtgggcgttg ggcgagcaca
     3121 agagaccaac gggcctagcc taactccttc accgagcaca gaccgatctc cctttcttct
     3181 atgcgcactg tcctttcttt gaaatcattt ttcgatccat gcgtccttgt tgtagagtag
     3241 ccgtatgcaa ggacaccccg tagggctgga tgggcgtcga ccgtctgccc tgaatcgctt
     3301 tcgacgacat ccaagagtcc gcatcaatac gcccttttct tgtgctctct ccccggtccg
     3361 ggcgcgaggc tggctgagaa aacccatcat caacgtcggg ttggtctatg acctttctgt
     3421 tcggggcgcg cgggtcagca ccgaagctcc cttcaagccc ggggatcagg tggccttgag
     3481 ccttcgcctc ccgagacaaa tccgttccgc cgagattgcc gtggcaacgg tgcggtggac
     3541 aaaggatcaa ttctatggac tggcatttcg ccgcctatcg ctcgatgcgt atgatcgatt
     3601 gaagaaattc gtggccattg cctcgcgagc agtcgcgtaa ctccatggcg caactggaac
     3661 gacagcctca gccgagcgtg gagctgcgac gctatcctcg tcatcgagtc tccgctccct
     3721 tcccttgcgc gttctccctc ctcgggctaa aacgctggac cgctgtcgac cgcaacgggc
     3781 tgggcgttgt gctggacgtg tcgttgggag gcgcgaaggt catgagcacc gcggcaatga
     3841 accccggcga tcatctggcg gtcagctttc gaatgcctga tcaagcctcc gccctgcagc
     3901 tcgacgcgaa tgtcaaatgg gttaatgatc agctatttgg actggagttt ctatcagtcc
     3961 ctctttcggc agagtctcgc ctcaagaagt tcctttcacg ctcgccacac acctgctcct
     4021 gacgtttccc gtactctgga tcagtaatcg tcccgtcctg taaggccgag accggttcgt
     4081 tcacgtgtgt ggttcgctcg gaagaagagg gaagaggatc tcagccgtgg agttgagatc
     4141 ctctccttac ttgatggcga cggctttcac ttctttgaac atcgtgagtc cctgtaaaca
     4201 cttctggatg ccgtcctgca tgag
//
LOCUS       DASTQY010000205        13242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_424489, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000205 DASTQY010000000
VERSION     DASTQY010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 13242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13242
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424489"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VFR82_06855"
     CDS             <1..633
                     /locus_tag="VFR82_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4501355.1"
                     /translation="IEDFDESAEELISQVFGAASDEAEAYFYAKNGESALLPEEAQEN
                     GTHNIERESLQQRRQVLESCMADLEMRRLIQSAKQSNGKGMLQAKVEDYMSHDVRSIH
                     RAATIKEAGLLLQKYKVGSLIVDDGSRYIGIVTDSDLSRKAVAKGLDPNTTTVTTCMS
                     KSLVTIEEDELLSEAMNLMKKQGIRHLPVTADGTIIGVLSLADLLRAFMD"
     gene            complement(623..1702)
                     /locus_tag="VFR82_06860"
     CDS             complement(623..1702)
                     /locus_tag="VFR82_06860"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU4501356.1"
                     /translation="MIPQIAWKAVLEHDSRFDETFVYAVASTRVYCRPSCPSRRPDRH
                     RVTFFPSSEQAEAAGFRACLRCIPGSVRGTQTERLVEQVRQHLDGHSDEPATLTSLAA
                     LVGMSPFRLQRAFTRVFGFSPKTYQNAKRIQQFRQALERGAGITDASYQAGFGSSSRA
                     WEQAWRHMGMTPSAFRSGGKGVIMHYASMRTPVGFLLIAATERGVTSVMLGGREPLLV
                     TKLRRQYPNALLRRNPKRLVPYRQALARCLNGMPAGAGLRFDVKSTAFQFQVWTALQQ
                     IPAGSTRTYREIASTIGRPAVSRAVARACATNPLALLIPCHRVVGQDGRLAGYRWGIR
                     RKKELLRLEAARRQQAAPQTGRPNP"
     gene            2429..3625
                     /locus_tag="VFR82_06865"
     CDS             2429..3625
                     /locus_tag="VFR82_06865"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HEU4501357.1"
                     /translation="MSRVYSAGVVLVLALFALACNDGDDSGSGTPNQPPSFNNTVLML
                     APLADGSGDVYVGGDFTTYDGAQAIRIVRLNGDGTIDPGFATGLGFNGSVRSIVPAGT
                     QGATIYVGGDFTTYNGIQRSRIVRLTANGAFDPTFAIGTGFDNTVHVIASTGDGTGDI
                     YVGGAFTNYNGIVANNLVRLNADGTVDSTFFMGIGFNNTVFTIMPTTDGSGDLYVGGA
                     FTSYKQVLANNLVRLNEDGSLDATFLTGMGFDNAVQTIAPADSGGLYVGGAFTNYNGT
                     LANGLVRLNSNGVINPFLATGKGFNNTVFHVVPAADASGDLYVGGSFTTYNDMQANQL
                     VRLNQNGTLDLPFATATGFGNTVLRVLPVGDASGDVYAGGQFVQYQSTPIGRFVRLTS
                     TGAFVH"
     gene            complement(3943..4533)
                     /locus_tag="VFR82_06870"
     CDS             complement(3943..4533)
                     /locus_tag="VFR82_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEU4501358.1"
                     /translation="MIDTSQALFLFEVLGVLALPGPTNSLLFVSGVSRGFQASLTLVL
                     AELGAYLLSLSALVFVLVPASQSHSAISHLLHLLCSTYLVYVAYWLWRSDERGVNASH
                     PITFPRVFLTTLVNPKNLIFAFGVFPVPSAPFEGMIPYFASFSVICAGVACCWIAAGM
                     VLHSTGAHKPHLNWFYRGEACLLVGFAVLILFSAYY"
     gene            4802..6619
                     /locus_tag="VFR82_06875"
     CDS             4802..6619
                     /locus_tag="VFR82_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU4501359.1"
                     /translation="MLLSCESISKSYGVKPLFANLSLGLSEGDHVGLIGPNGSGKSTL
                     LKILAGLDEPDCGTRSVRRQVRIGYVPQEPSFSVSHTVEDALAQVLLDEGLDPHEHGG
                     RIAKALSLGGFVRADQAVATLSGGWKKRLAIARALMLEPDVLLMDEPTNHLDVEGILW
                     LEGVLQAEPHAFLVVSHDRKFLESVASRIWELNRCYPNGVFQANGRYSDLLEQREAAL
                     QAQADYQASLANRVRREVEWLRRGPKARTTKAKARVDAAGQLIDELQTLESRQGQSSA
                     GIDFTASGRKSKQLLVATGLGKSLGGRPIVTSLDFQLGPGERIGLLGPNGSGKTTLLK
                     LLAGTLQPDSGTIARADRLRVVTFEQHRESLDQQATLRHSLAPAGDTIVYQDRSLHLI
                     SWAKRFLFKPEQLDLPVSRLSGGEQARLLIARLMLQPADLLILDEPTNDLDIPTLDVL
                     EDSLLEFTGALVLVTHDRWLLDRVSTRLLALDGTGRAEWFADYAQWEAAQARSAGDAR
                     RPATALAASTVKTPLKRKGLSYREQQEWEKIEEQILKAEAVVAACQSAVNDPGIASSA
                     EALQERYNALHAAQADVERLYARWAELDEKRTQAVKSAP"
     gene            complement(6735..7061)
                     /locus_tag="VFR82_06880"
     CDS             complement(6735..7061)
                     /locus_tag="VFR82_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501360.1"
                     /translation="MRIVLICLLTLILSGCINISTELQPSNTDVKGVLLGEDCTPIVL
                     GMGLGTNTIEGAKRDGRPMGNVGEFRSRVKLNERTVINRIRTIQITSFYVLLVGSSCI
                     QVVGEP"
     gene            complement(7522..9795)
                     /locus_tag="VFR82_06885"
     CDS             complement(7522..9795)
                     /locus_tag="VFR82_06885"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501361.1"
                     /translation="MASSDREAILSSLTSLSTDVPSDVLRDFVTRMDHEYLRRVPLQT
                     IAQHVRLAARLTPEHLCECTVAELGDGHFDLTVVAYDYFAEFATICGLLSAFGLNIEE
                     GQIYTFTETPVSNPATRPAYGEGRSQRPKLRPGLSRKKIVDVFRVQPVGGASFTPADQ
                     ARFTRELRTLIGLLDDDKFEQARHAVNRQLVEQLGKRRGSFSGLLHPVQITFDNSQSP
                     SDTIMDIRSDDTPAFLYAFANALAMRNVYIARAQITLEGGKLHDRFAVRNRHGQKLTD
                     HVDQQQLRLTAVLIKQFTHALTWAPDPTKALEAFDQFLDLTVQETKGKAREKALAFLS
                     DKKTFPLLARLLGASDFLWEDFLRRQHDHLLPLLQDYRDAPLIKPQTAIRKALDRVVE
                     RHKDEAGRKEALNRFKDQELFRIDMKHIVEPDSSLADFSLALTDLAEVIVQRSLKDCQ
                     AKLGKQYGAPRLANRKPCPFAIMGAGKFGGRELGYASDIEVLFIYGGAGRTDGKHRIE
                     NSEYFERLSQELLQWIEAKQEGIFHLDVRLRPHGGKGTLANPLDEITAYYSDNGLAAP
                     FERQAMIKLRHVAGDEALGKQVEAHRDRYVYSGAPWDLVAALDLRRQQLKQLVERGTV
                     NAKLSHGGVVDIEYAIQYLQLMHGHRHAVLRTPNAMQALAGLVESGTVSRHDGENLRK
                     AYLFLRMLIDGLRMVRGHAKDLVIPPADSEEFIFLARRVGYTTDDWQAGARHLKTDID
                     EHMGRVREFWERTFGKL"
     gene            10147..11838
                     /locus_tag="VFR82_06890"
     CDS             10147..11838
                     /locus_tag="VFR82_06890"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501362.1"
                     /translation="MESEIGKPDPTPLLLASERAPQDIRVILSAYGLTDIEQADRNLQ
                     AMAGQPHERHQLVGILPILMESVARTADPDQALNHWERLLESVTRSTFLDYLRSSPRM
                     LELLSTIFGNSDSLAFTIIRDPTLVYWLAEEEVLSRPSTRSGLERALRKQLSRLTVKE
                     LKLAVLRRFRRREMLRIGVRDLLRLAKVPETTASLSDLAALLIEAAYEIVDADLGRQY
                     GKPMHKNRQGRWVETGFAVMGMGKLGGHELNYSSDVDLIYVYASHEGETRAVPARSVK
                     AVAAGISNEEYFEILSRELTKALSEQTREGYVFRVDLRLRAEGSVGQLARSVDDYRKY
                     YFTRGRIWERLALLKAWPVAGSREVGKAFLRTVRPFILGQGTRKSGLAGAMAVVRDVR
                     TVKEMIDAKMADRGHEHRNVKLGTGGIREIEFLAQTIQVLAGQQDPALLHRSTLDSLK
                     AFARKKLLSTKERDALSAAYLFLRDIEHKLQMVHDLQTHSLPDDALELERCAIRSGYQ
                     GTNRPVIASRFQADYRSHTNVVHDLFRSFFYETEVSPLLKATLKAMAVRRPAQGA"
     gene            complement(11826..12182)
                     /locus_tag="VFR82_06895"
     CDS             complement(11826..12182)
                     /locus_tag="VFR82_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501363.1"
                     /translation="MIVEVEANENCEASLFARFKGAGQPRQVHQVRLFDRNPSGEWYW
                     VTGWSNNAEAPASAAYAQLVEDSGSGLAHLVYGGIYGLRFKPVAVDEPWSLDSPHQWG
                     ETHLSLATDRDLRYAP"
     gene            complement(12306..12917)
                     /locus_tag="VFR82_06900"
     CDS             complement(12306..12917)
                     /locus_tag="VFR82_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEU4501364.1"
                     /translation="MKQIKAQIEETTSDYDREKLQERLAKIVGGVAVINVGAATETEM
                     KEKKARVEDALHATKAAVEEGIVPGGGTAYLRCIKALDTIKDVPAEQKVGLDIIRRAL
                     EEPIRQIVNNAGGEASVVVGKVREDKNVNAGYNAATEEYVDMIKAGIIDPTKVSRTAL
                     QNAASVAALMLTTEVMVTDIPEEKKEPMGGGGHGHNHGMEGMY"
     gene            12918..13242
                     /gene="groEL"
                     /locus_tag="VFR82_06905"
                     /pseudo
     CDS             12918..13242
                     /gene="groEL"
                     /locus_tag="VFR82_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; many bacteria have multiple
                     copies of the groEL gene which are active under different
                     environmental conditions; the B.japonicum protein in this
                     cluster is expressed constitutively; in Rhodobacter,
                     Corynebacterium and Rhizobium this protein is essential
                     for growth; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
BASE COUNT         2827 a         3785 c         3924 g         2706 t
ORIGIN      
        1 atcgaagact tcgatgagtc ggctgaagag ctgatcagtc aggtgttcgg agccgcttcg
       61 gatgaagccg aggcctattt ctatgccaag aacggcgagt cggccctgtt gccggaggaa
      121 gcgcaggaga acggaacgca caatatcgag cgtgaaagcc tgcaacagcg ccggcaagtg
      181 ctggaaagct gcatggccga tctggaaatg cgtcgcctga tacagtcagc caaacagtca
      241 aacggaaagg gcatgttgca ggcgaaagtg gaagactaca tgtcccacga tgtgcgcagt
      301 atccatcgag ccgccactat caaggaagcc ggtctgttgc ttcagaaata caaggtggga
      361 tcgctgatcg tggacgacgg gtcgcgctac atcggcatcg tcacggattc cgatctcagc
      421 aggaaagccg tggccaaagg gcttgatccg aacaccacca ccgtgacgac ctgcatgagc
      481 aaatcgcttg tgacgataga agaagacgaa ttgttgtcag aagcgatgaa cctcatgaaa
      541 aaacagggca tacgccattt gcctgtcacg gctgacggca ccatcatcgg tgtgctttcg
      601 cttgccgacc tccttcgcgc cttcatggat taggacgacc tgtttggggg gcggcctgct
      661 gacggcgggc tgcctccagt ctcagtaatt ccttttttcg tctgatgccc caccgataac
      721 ccgcaaggcg gccgtcttgg ccgacgaccc gatggcaggg aatgagcagg gcgaggggat
      781 tggttgcgca ggcacgggcc acggcacgcg aaactgccgg tcggccgatc gtgctggcaa
      841 tctcccgata cgttctggta gacccagcag ggatctgttg cagcgccgtc catacttgga
      901 attgaaaggc tgtcgatttc acgtcaaatc tcaggccggc tcctgcaggc attccgttca
      961 ggcaccgagc caaagcttgc cggtagggga ccaatcgctt gggatttcgg cgcaacaacg
     1021 cattcggata ctgtcgtctc agtttagtca ccaacaaggg ctcccttcct ccaagcatga
     1081 ccgaggtgac accgcgctca gtggcagcga tcagcaagaa gccgactggt gtgcgcatgc
     1141 ttgcataatg cattatgact ccctttccgc cagaccgaaa cgccgacggc gtcatgccca
     1201 tgtgccgcca tgcctgctcc cacgcccggc tgcttgatcc aaacccggcc tggtatgagg
     1261 catcggtgat tccggctccg cgctctaagg cttgcctgaa ctgctgtatt cttttggcat
     1321 tttgataggt cttaggcgag aacccgaaga ctcgggtgaa ggcgcgttgc agtcgaaagg
     1381 gactcattcc gacgagcgcc gccaggctag tgagagtcgc cggctcatcc gagtgcccgt
     1441 ccaaatgttg ccggacctgt tcaaccagcc gctcggtctg agtgccgcgt actgaacctg
     1501 ggatacagcg taggcaggcg cgaaagcctg cggcctcagc ctgttccgag gaaggaaaaa
     1561 atgtgacgcg atggcgatca gggcgccgtg agggacagga agggcggcaa tagacgcggg
     1621 tcgaagccac cgcatagacg aacgtctcat caaatcggga atcatgctcg agcaccgctt
     1681 tccaggctat ctgcggaatc atcggtattc gtcctcctcg tggcactgat gataccgttc
     1741 gtcgagggcg ggcgctatcc gattcttgcg ctcaaagtct gacgaagcac gatcactggc
     1801 cggggaaacg atgaaagggc tgagcgaagg aagatcagct ccccgaacga ttccgcaggt
     1861 aaaatcgcgc gccgcatcga ttgcagcgcc aagggaacat tcccaagagg cgaaacaaca
     1921 aatcctgcca cccgtgccgg gtcgtgcgat ggcactcttc tgaagaacag taccgacaat
     1981 gcatccggga cgctgggctg tatccgacac cttgctccag catgagccac cggctggcac
     2041 tcactgccct cgtgccgtcg ctaggatcgg cacgagatga aaagatctaa gacgagtctt
     2101 gattcatcgc cctgcgggac agcagccatt tggccatcta cttttccagg accgagagag
     2161 tagggcctgc atgcgccatt ggtggaccgc cgccagacgc tgtcacgacg atggaaacga
     2221 attaatctgt tgtctaccgc tgttcatatg ccggaaggct agtcttcggc agaagccgag
     2281 tcaacggggt aagtgcaagg gataggcgaa cttgtgcccc ctgttcgagg ggtagacggc
     2341 tggcagactg cttacataga cttcccaggc gtcgtcggcc tattgctgca aagccgcttc
     2401 tgtggctcct cgggaggttg tcgaaagaat gtcacgcgta tatagcgcag gcgtcgtctt
     2461 ggtcttggcg ctgtttgccc tggcttgcaa cgacggcgac gatagcggta gtggcacccc
     2521 caaccaaccg ccctcattta ataacacggt cttgatgctc gctcctctcg ctgatggcag
     2581 cggggatgtg tacgtgggag gcgactttac gacatacgat ggcgcacagg cgattcgtat
     2641 cgtgcgtctc aatggggatg gcaccatcga tccagggttt gcaacaggcc tcggctttaa
     2701 cggcagcgtg cgcagtattg tgccggctgg gactcaaggc gcgacgatct atgtgggagg
     2761 agactttacg acctacaacg ggatacagag gtctcggatt gtgcgcctca cagccaatgg
     2821 ggcgtttgac ccgacctttg ccataggaac cggattcgac aacacggttc atgtcatcgc
     2881 ttctacagga gacggaaccg gagatatcta tgtcggtgga gccttcacca actataacgg
     2941 gatcgtagcg aataaccttg tgcgtctgaa tgccgacggg acggtggatt cgaccttttt
     3001 catggggatc ggtttcaaca ataccgtttt caccatcatg ccgacgacgg atggaagtgg
     3061 agatctctac gtgggagggg cctttaccag ctacaagcag gtattagcca acaacctcgt
     3121 acggctcaac gaagatggat cccttgatgc gacgtttttg acagggatgg gatttgacaa
     3181 tgctgtccaa acgattgctc cggcagacag cgggggtctg tatgtcggcg gcgccttcac
     3241 gaactataac ggcacgctgg caaatggact cgtacggctg aactcaaatg gggtgatcaa
     3301 cccgttcctt gcgacgggga aaggcttcaa caatacggtc tttcatgtcg tgcctgccgc
     3361 cgatgcaagc ggcgatctct acgtcggagg gtccttcacg acctacaatg acatgcaggc
     3421 caaccagctg gtacggctca atcagaatgg gacgctcgat ttaccctttg ccacagcgac
     3481 aggcttcggc aatacggtgc tccgcgtgtt gcccgttgga gacgcaagcg gggatgtgta
     3541 tgccggaggc cagttcgtgc agtaccagtc cacgcccatc ggcagatttg tgcgcctcac
     3601 atccactgga gccttcgtcc actgagagat ttctcgcaaa cgtgcgctgc ggcggctccg
     3661 aaccccaact taagactccc accccaaacg gcgcagattt taaaaatgac agcctcgatc
     3721 gaggtttcga agattccgag tctatggtcg gagtttggct tgtctccggc atgcctgttt
     3781 tcggcagagt ccctcaaaag cgtacgattt gaaaataaac cttgggaatt ggacaaggga
     3841 tttagaaaga gagacacgcg ccgacaaagc tttctcgacg agcacgcgca ttaatcgccg
     3901 cctaggaacg tctctagcag aacaccacct gcctctctcg atttaatagt acgcggagaa
     3961 gaggatcaag accgcgaatc ccacgagcag gcacgcctcg ccgcggtaaa accaattcag
     4021 gtggggcttg tgcgcgccgg ttgaatgaag caccatgcct gctgcaatcc agcagcacgc
     4081 gactcctgcg cagataaccg aaaagctagc aaaatagggg atcattccct cgaacggcgc
     4141 actgggaaca ggaaagacgc caaaggcaaa aatcaggttt ttggggttga ccagtgttgt
     4201 caaaaaaacc cggggaaagg tgatgggatg cgaagcattg actcctcgct cgtccgatcg
     4261 ccagagccag taggccacat agacaaggta ggtgctgcac aacaggtgga gcaagtgact
     4321 gatagccgag tggctctgag acgcaggcac aaggacgaag accagcgcag atagcgaaag
     4381 aaggtacgcg cccagttcgg cgagcacgag agtgaggctt gcttgaaaac cacgtgacac
     4441 tccggagacg aacagaagcg agttcgtcgg ccccggaagg gccaggacac ccaagacttc
     4501 gaagagaaag agcgcctggc ttgtgtcgat cacaagaaaa gcatgtatcc gagaacgcgc
     4561 ttggagtcaa cacggtcgtt tgtgttgagg gaatccgtgc gcgcgaaatg attcctgacg
     4621 ccgtttcttt tacgccttcg cgagcgtgaa gctccagatg catgttgtgc caattgactt
     4681 ctcgtgatca ggcacgtaga agaaaggcct ggcaccactg cccgccctgg cttgagtcac
     4741 cctgtctgtg cgttcatcac ctgatcggag aacctgttcg ggtcagacta tgccgccgac
     4801 catgctgctc agctgtgagt ccatcagcaa aagctatggg gtgaaaccgc tcttcgccaa
     4861 cctgtccctc ggcctctccg aaggcgatca cgtcgggctg atcggtccca acgggtccgg
     4921 caaatcgacg ttgctcaaga tattggcagg actcgatgag ccggattgcg gcacccggtc
     4981 ggttcggcgc caagtccgca tcggctatgt tccgcaggaa ccgtcgtttt ccgtgtctca
     5041 cacggttgaa gacgcgcttg cccaagtcct cctcgacgaa ggtctggacc cccacgagca
     5101 cggcggtcga atagccaaag ccctcagcct cggaggcttt gtacgtgccg accaggcagt
     5161 cgcgacactc tccggagggt ggaagaagcg tctagcgatc gcgcgggcgt tgatgctgga
     5221 gccggatgtg ttgctgatgg atgaaccgac caaccatctg gacgtcgagg gaattctgtg
     5281 gctggaagga gtgttgcagg ccgaacccca tgcgtttctc gtcgtcagcc atgacaggaa
     5341 gtttctggaa tctgtggcct cccgcatctg ggaattaaac cgctgttacc cgaacggcgt
     5401 gtttcaggca aacggccggt atagtgattt gttggaacag cgcgaggcgg ctctgcaggc
     5461 gcaggccgat tatcaagcct ccctggcaaa tcgcgttcgg cgtgaagtgg aatggctccg
     5521 gcgtgggccc aaagcccgta cgacgaaggc caaagcgcgg gtagacgcag ccggacagct
     5581 gatcgacgag ttgcagacac tcgaatcgcg gcagggacag agttcagcag ggatcgactt
     5641 cacggcgtcg ggacgaaagt ccaaacagct tctggtggct accgggctcg gcaaatcgct
     5701 tggcgggcgg cctatcgtca cgagcctgga ctttcagctt ggccccggcg aacgtatcgg
     5761 gctccttggc cccaacggca gcggtaagac cacactgctg aagttgctgg cgggcactct
     5821 gcaaccggat agcggcacca tcgcgcgtgc cgaccggctg cgcgtcgtga cattcgagca
     5881 acaccgcgaa tcgctggacc agcaggccac gctgcggcac tcccttgcgc cggccggtga
     5941 cacaatcgtt tatcaagacc ggtcccttca tttgatctca tgggcgaagc gttttctctt
     6001 caaaccggaa caactggacc taccggtctc ccgcctgtcc ggcggagagc aggctcgact
     6061 gctcatcgca cgactgatgc tccagccggc cgaccttctg atcctggacg agccaacgaa
     6121 cgatttggat attccgacgc tcgacgtgct ggaagacagc ctgttggaat ttaccggagc
     6181 gctcgtcctc gtcacgcacg accggtggct gttggaccgc gtctccaccc ggctgctcgc
     6241 gctggacggc acgggccgcg ccgaatggtt cgccgactat gcccaatggg aggccgcaca
     6301 ggctcggagt gcaggcgacg cccggcgacc cgcaactgca ctcgccgcga gcacagtgaa
     6361 gactcctctc aaacggaaag ggttgtccta tcgggagcaa caggagtggg aaaagataga
     6421 agagcagatt ctcaaggccg aggcggttgt cgctgcttgt cagtcggcgg tgaacgatcc
     6481 tggtatcgct tcctccgcgg aagccctcca ggagcggtac aacgcgctcc acgctgcaca
     6541 agccgacgtc gaacgtctct acgcacgctg ggccgaactc gatgagaagc gcacccaggc
     6601 cgtgaagagc gcgccatagt accggcgcca atggactcgg cgaaacgtga tgcgaaaccg
     6661 atcacctcac agaaagtgtc cgttatggat gccgaagtcg gcgaaagtcg cacagacgaa
     6721 tcaggcaggg acgatcatgg ctcgccgact acttgaatgc agctggaacc aaccagcaat
     6781 acataaaaac ttgtgatctg aattgtgcgg attcggttga taaccgttcg ttcattgagt
     6841 tttacacgag agcggaactc gcccacgtta cccattggcc tgccatccct tttcgcgcct
     6901 tcaatggtgt ttgtgccgag tcccatgccg aggacaatag gcgtgcagtc ttcaccaagc
     6961 aggacgcctt tgacgtcggt attgctcggt tgtagttccg tggagatatt gatgcaaccc
     7021 gacaggatca gcgtcaacag acagatgagc acaattcgca tcagaccgac tccttcctaa
     7081 gatgggaagc ctgaaaagcc agacagggta ctggtgtgtg aagggatgtg tcaatccagg
     7141 caatgctaat ctttcctgcc ggacatcacc taatgctttg ttcaactgtg agtattgtgc
     7201 gagttgcgat ggcggattga agtctgaggg gaaggtttat ctagtttagt acagcttcca
     7261 tccaaattta cctcgacgac ccgaccagtc aagcgccgct tgcttaatca atctcggaaa
     7321 tctataccga cttgccgatg tctggccggc taaaatccgc tgtgcattat gacatttcga
     7381 gaacgttcaa taagtgggga ggattcgggg cgaccggcga ctcgagtgcg cgcctggacg
     7441 aacacctccc gctatgtgaa catccaaatg gttctcgtgt gtcgcgttcg gcgagcacag
     7501 ggaccgacca ggttagcttc ttcataattt tccgaacgtc cgctcccaaa actccctcac
     7561 ccttcccata tgctcgtcta tatccgtctt caaatgcctg gccccagcct gccaatcatc
     7621 ggtggtgtac ccgacgcggc gtgccaggaa gataaactct tctgaatcgg caggcgggat
     7681 gaccaggtcc ttggcatgac cccgcaccat ccggagtccg tcgatcaaca tgcggaggaa
     7741 caaataggcc tttcgaagat tctcgccgtc gtgccggctg acggtcccgg attccacgag
     7801 ccccgccaga gcctgcatgg cattcggcgt gcgcaacacc gcatgccggt ggccatgcat
     7861 cagttggaga tactggatgg catactcaat atccaccacc ccgccgtggc tgagtttcgc
     7921 gttcactgtg ccgcgttcga cgagctgttt cagttgctgt cggcgcaaat cgagtgctgc
     7981 cacaagatcc cacggcgcgc cgctgtagac ataccggtcc cgatgggctt cgacctgttt
     8041 gccgagtgcc tcatcgccgg ccacatggcg cagcttgatc atggcctgcc gttcgaatgg
     8101 cgcagccagt ccgttgtcac tgtaatatgc cgtaatttcg tccagcggat tggcgagagt
     8161 gcctttgcct ccgtgaggcc ggaggcgcac atcgagatgg aagatgcctt cctgtttggc
     8221 ctcgatccat tgcagcaatt cctgactgag ccgttcgaag tattcactgt tctcaatgcg
     8281 gtgctttccg tccgtgcgcc cggcaccgcc atagatgaac aagacttcga tgtcggaggc
     8341 atagcccaac tcacgaccgc cgaatttgcc tgcgcccata atcgcaaaag gacagggctt
     8401 cctgttcgca agccgcgggg cgccgtattg tttacccaac ttggcctgac aatctttcag
     8461 actgcgttgg acgatgacct ccgccaagtc ggtcagggcg agcgaaaagt cggccaggct
     8521 gctatctggc tcgacgatgt gtttcatatc gatgcggaac aattcctgat ccttgaagcg
     8581 gttcaacgcc tccttacgcc cggcttcatc tttgtgccgt tccacgacac ggtcgagcgc
     8641 cttccggatc gccgtctgcg gtttgatgag cggcgcatca cgataatcct gaaggagggg
     8701 caacagatga tcgtgctgcc gccggagaaa atcctcccag agaaaatcgc tggcgcccaa
     8761 cagccgggcg aggaggggaa aggttttctt gtcgctgaga aaagcgaggg ctttctctct
     8821 cgcttttcct ttggtttcct gaacggtgag gtcgagaaac tggtcgaacg cttccaaggc
     8881 ctttgtgggg tccggcgccc aggtcagagc atgcgtgaat tgcttgatca aaacggcggt
     8941 caggcgcaac tgctgctggt ccacgtggtc ggttagcttc tgcccgtggc ggttcctgac
     9001 ggcaaagcgg tcatgcagct ttccgccttc cagcgtgatc tgtgccctgg cgatatacac
     9061 attgcgcatg gccagtgcgt tagcgaaggc ataaagaaac gccggcgtgt cgtccgaccg
     9121 gatgtccata atggtgtcgc tcggcgattg gctgttatcg aatgtaattt gaaccggatg
     9181 gagcaggccg ctaaaggagc cgcgccgttt gccgagctgt tcaaccagct gccgattgac
     9241 cgcgtgccgg gcttgctcga acttgtcgtc atccagcaag ccgatcaagg tgcggagttc
     9301 gcgtgtaaaa cgggcttggt ccgctggggt aaacgacgcg ccgccgaccg gttgaactcg
     9361 aaaaacgtcc acgatctttt ttcggctcaa accggggcgc agcttgggcc gttggctccg
     9421 cccttctccg tatgcaggcc tcgttgctgg gttggaaacc ggcgtctcgg tgaacgtata
     9481 aatctgccct tcctcgatgt tcaacccgaa tgccgacagc agtccgcaga tggtcgcgaa
     9541 ttcggcaaaa tagtcatagg cgacgaccgt cagatcgaag tgcccgtcgc caagttcggc
     9601 gactgtgcat tcgcaaagat gctccggcgt caatcttgcc gccaaccgca catgctgcgc
     9661 gatcgtctgc agcgggaccc gccgaagata ttcatggtcc atccgggtga cgaaatcacg
     9721 aagcacatct gatggcacgt ctgtagagag cgaggtgaga gacgatagaa tagcttcgcg
     9781 gtcggaggag gccattgggg cgtcagtata tcgcaggacc gttcattgtg cgtaatctgg
     9841 gctgacagta taatgccgcg ccgacaggat ggtgaaaaag gacgaccggt ttcgttctcg
     9901 cgtcccagcg ctcgatatac gcaccggaaa gagctgctcc ggcagctcgg ggtgggcggg
     9961 tgagccggga aagagctgct ccggcagctc ggggtgggtg ggtgagtgag ataaataccc
    10021 ttttgataat cctgcgggac gttgacattc atcaaccaac gtagtgctga ggcggttttg
    10081 gggactaaaa aaggagagcg tccggcacta agccggagaa caccttcgaa gtcggcacat
    10141 gtgtcgatgg agtcggagat tggaaagcct gatcccacac cgttgttgct ggcatctgaa
    10201 cgcgcccccc aggacatacg cgtcatcctg tcggcctacg ggctgacgga tatcgaacag
    10261 gccgaccgca atctgcaggc gatggccggc cagccgcatg agcggcacca gctcgttggc
    10321 attctgccga tcctgatgga gtcggttgcg cgcaccgccg acccggacca ggcgttgaat
    10381 cattgggagc ggctcctcga gagcgtgacc cggtcgacct ttctcgatta tctgcgcagc
    10441 tcgccgcgca tgctcgagct gctctctacc attttcggca atagcgattc actcgccttc
    10501 acgattattc gcgatcccac gctggtgtac tggctggcgg aagaagaggt cctctcgagg
    10561 ccgtcgacgc ggtcgggctt ggagcgcgcg cttcggaagc aattgtcccg cctcaccgtg
    10621 aaagaactga agctggccgt gctgcgccgc tttcgacgac gcgagatgct tcgaatcggc
    10681 gtgcgggatc tgttgcggct ggcgaaggta ccggagacga cggcttcact gtcggatctg
    10741 gccgcgctgc tgatcgaggc cgcctatgaa attgttgatg ccgatttagg ccggcagtac
    10801 ggcaagccca tgcacaagaa ccggcagggc agatgggtcg agacaggatt tgctgtcatg
    10861 gggatgggca agctcggcgg gcacgagctg aactacagct ctgacgtgga tttgatctat
    10921 gtgtacgcct cgcatgaagg ggagaccagg gcagtgccgg caagatccgt caaagcggtg
    10981 gctgcaggca tctccaacga agagtatttc gaaattctct cgcgcgagct cactaaggcg
    11041 ttgtccgagc aaacccggga gggctacgtc tttcgcgtgg acttgcgctt gcgcgcggaa
    11101 gggtcggtcg gacaactggc ccgatcggtg gacgactatc ggaaatatta tttcaccagg
    11161 ggacggatct gggagcgact cgcactattg aaagcctggc cggtagccgg atctcgggaa
    11221 gtggggaagg ccttcttaag aactgtcagg ccgtttattt tgggacaggg cacccgcaaa
    11281 tccgggctgg caggcgcaat ggccgtcgtc cgggatgtgc ggacggtcaa ggaaatgatc
    11341 gatgcgaaga tggcggatcg ggggcacgag catcgcaacg tcaagctcgg caccggtggc
    11401 attcgggaaa tcgagttcct cgcgcaaacg atccaggtgc tggctggcca gcaggatccg
    11461 gcgttgttgc accgcagcac cctcgactcg ttgaaggcgt ttgctcgaaa aaagctcctg
    11521 tcgacgaagg agcgcgacgc gctgtctgcc gcctatctgt ttcttcgaga catcgaacac
    11581 aaactgcaga tggtccatga tctacagacc cactccctcc cggatgatgc cctggagctt
    11641 gaacgttgcg ccatccgttc gggatatcag gggaccaatc gtccggtcat cgccagcagg
    11701 tttcaagccg actatcgttc gcacacgaat gtcgtccatg atctgttccg gtcgtttttc
    11761 tacgagaccg aggtgtcgcc actgctcaag gcgacgctga aggcgatggc ggtacgccga
    11821 ccggctcagg gggcatagcg caaatcacgg tctgtggcca gcgacagatg cgtctctccc
    11881 cattgatgcg gactatcgag gctccacggc tcatcgacgg cgacaggctt gaaacgtagc
    11941 ccatagatcc cgccatagac gagatgggcc agccctgagc ctgaatcttc gaccagctgc
    12001 gcgtaggcgg cggaagcggg cgcctccgcg ttgttgctcc agccggtgac ccaataccac
    12061 tcgcccgagg gattccgatc gaagagccgc acctgatgga cttgccgcgg ttggccggca
    12121 cctttgaacc gcgcgaacag gctggcttcg cagttctcgt tggcttcgac ttcgacgatc
    12181 atgctcgcgg ttcctacggt cacgttcatt ctagcgcagc aatctcaaca aagaaaaagg
    12241 gcccggcggg aaccgccgga ccctttattc aaccagcagc tatcagacga tagctgtgtt
    12301 ggctcttagt acatcccttc catgccgtgg ttgtggccat gcccgccgcc gcccatgggt
    12361 tccttcttct cttcagggat gtcggtgacc atgacttcgg tcgtcagcat caacgccgcc
    12421 actgacgccg cgttctgcaa cgcagtccgc gacactttgg tcggatcgat gataccggcc
    12481 ttgatcatgt cgacatactc ttcggtcgcc gcattgtagc cggcgttcac gttcttgtcc
    12541 tcgcggacct tgccgaccac gaccgaggct tcaccgccgg cattgttcac gatctggcgg
    12601 atcggctctt cgagcgcgcg ccggatgatg tccaagccga ccttctgctc cgccggaaca
    12661 tccttgatgg tatccagcgc cttgatgcag cgcagataag ctgttccgcc gcccggaacg
    12721 atgccctctt ccaccgccgc cttggtcgcg tggagggcgt cctcgactcg ggctttcttt
    12781 tccttcattt ccgtctccgt cgcggcgccg acattgatca ccgccacacc gccgacgatc
    12841 ttggccagcc gctcttgcag cttctcgcga tcgtagtcgg aggtcgtttc ctcgatctgg
    12901 gccttgatct gcttcacccg ggcttcggag accggcgcaa ggccatgctg caagacattg
    12961 ccgttctcac cggcggtgag atgatctccg aggatctcgg catcaagcta gagaatgtaa
    13021 cggtcgagat gcttgggcac gctaagcggg tctcaatcac taaagatgac accacgatcg
    13081 tcgatggcgc cggcggcaag aaggatatcg aggcccgcat tgcccagatc aagcaacaga
    13141 tcgaggatac cacatccgac tacgatcgcg agaagctgca ggagcggctt gccaagctcg
    13201 ccggtggcgt tgctgtgatt aaggtcggcg gatcgacaga aa
//
LOCUS       DASTQY010000206         2932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_853531, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000206 DASTQY010000000
VERSION     DASTQY010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853531"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFR82_06910"
     CDS             complement(<1..655)
                     /locus_tag="VFR82_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEU4501365.1"
                     /translation="MGQSPSRQHFPLAPTVVLYHAECADGFGAAWALWRRFADARFVP
                     VKHGSPPPDGLQGQRVVIVDFSYGRETLEMMAGDTDRLLILDHHITAEKALAGLPYAY
                     FDMKKSGAVLAWEWAQDHPGPWLLDYIQDKDLWTWALPMSREINAAVASHPFDFHLWS
                     TFKQKDLEQEGRAILRYEHELVNKLSGQAVLVEFRGETVPSVQSAILTSQIGERLSAE
                     "
     gene            complement(804..1847)
                     /locus_tag="VFR82_06915"
     CDS             complement(804..1847)
                     /locus_tag="VFR82_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEU4501366.1"
                     /translation="MKTTPSRSRTILSNDEILRQIRSLLECPEDDLSAGLLKELMAGI
                     IKLHDAHLEPLDLKIVNRAIKELRHAFRVFRDYHHRKKVSIFGSARTTADDPNYRIAF
                     DFCRSVVEAGFMVITGGADGIMRAAQEGAGRENSFGVNIMLPFEQGPNNTIADDPKLV
                     TFKYFFTRKLIFQKEANAIALFPGGFGTHDEGFEILTLAQTGKSEPQPIICLQAEGCD
                     YWDDWYGFIKRQMLDRRLISPEDLGLFKITSSVQEAIDEIRTFYTRFHSIRYVGRQLA
                     MRLAAPLSAEQVAAIHEQFADLLLEGTFELRGALPEELDEPDLKDLPRLVFASNRRSA
                     ARLRQLIDHVNRL"
     gene            complement(1936..2709)
                     /locus_tag="VFR82_06920"
     CDS             complement(1936..2709)
                     /locus_tag="VFR82_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4501367.1"
                     /translation="MLGPNEPSPQAHHPTPIVVAHMMTPGVVQVPGDVSVSEAALLLE
                     REHAPCLLVKETETVFGLMTPHDIVKKVVAQGLEPHDIEVRSIMTRPVQFIEYDELLE
                     EASTMMVATGASLLIVTKQNQPVGVLSAQDLMLTPARRLINIPTVVTIVDEAVPQRQA
                     HNATIVQLSHVSARIESASSLPPKTRVVLAFSLPGQHTPLTIHGKVLSSETLPSTTSH
                     ESAERTVAVEIQFSDLSASDLTRLKAWTLQAIPSSLDRP"
BASE COUNT          628 a          781 c          873 g          650 t
ORIGIN      
        1 cctcggcgga gagtcgctcg ccgatctggc tggtcaaaat ggcactttgc acagaaggga
       61 cggtttcacc ccggaattcg acgagcaccg cttgtccgga aagcttgttc accagttcat
      121 gttcgtaccg taaaatagca cggccttcct gctccaagtc cttctgcttg aaagtgctcc
      181 agagatgaaa gtcaaacgga tgcgatgcca cggctgcgtt gatctcgcgg ctcatcggca
      241 gggcccacgt ccacaaatcc ttgtcctgga tatagtccag aagccaggga ccaggatggt
      301 cttgcgccca ctcccaagcc aggacagccc cggacttttt catgtcgaaa taggcatacg
      361 ggagacccgc caacgctttt tcggcggtaa tatgatgatc gaggatgagc aaccgatctg
      421 tgtctcctgc catcatttcc agcgtctccc ggccgtaact gaaatccacg atgacgaccc
      481 gctgtccctg tagtccgtcc ggtggtggag acccgtgttt gactggaacg aatcgcgcat
      541 ctgcaaagcg ccgccacagg gcccaggcag cgccaaaacc atccgcacat tccgcatggt
      601 acaagacgac ggttggcgca agcggaaaat gttgtcgaga gggggattgg cccatggagt
      661 tcgagcatat cacgggattc tgggagacta aaggctcatt tgtggagaag attaaggaag
      721 aaagagcagg cgactggcag gaagcctttc ccctgccttg gtcgcggcag acagtcgcgc
      781 ggtctttggg attttgaggt cggttacagg cggtttacgt ggtcgatcaa ctgtcgcagg
      841 cgtgcggcgc tgcggcggtt ggaggcgaag accagacgcg gtaaatcctt cagatccggc
      901 tcatcgagtt cctctggcag cgcgccgcgc agctcaaatg tgccttcaag caacagatcg
      961 gcaaactgct catgaatggc ggccacctgc tccgcggata gcggagccgc aagacgcatg
     1021 gcgagttggc ggcccacata tcggatcgag tggaatcgcg tataaaacgt ccgaatttca
     1081 tcgatggctt cttgaaccga ggaggtgatt ttgaaaaggc cgaggtcttc cgggctgatc
     1141 aagcgacgat ccagcatctg ccgcttgatg aaaccgtacc agtcgtccca gtaatcgcag
     1201 ccctccgcct gaaggcagat gatcggctgg ggctcacttt tgccggtctg ggcgagcgtc
     1261 aggatttcaa acccttcgtc atgggtgccg aagccgccgg gaaagagcgc aatggcgttg
     1321 gcctctttct gaaaaattaa ttttctggtg aaaaagtatt tgaaggtcac caatttcgga
     1381 tcatcagcaa tcgtgttgtt ggggccctgt tcaaacggca gcatgatatt gacgccaaag
     1441 ctgttctcac ggccggcgcc ttcctgcgcc gcccgcatga tgccgtcggc tccgccggtg
     1501 atgaccatga agcctgcttc gacaacgctg cggcaaaagt cgaaggcgat ccgatagttc
     1561 ggatcgtctg cggtcgttct cgctgatccg aaaatgctga cttttttccg gtgatgatag
     1621 tctcgaaaga cgcggaacgc gtgacgcagc tctttaatgg cgcggttgac aattttaaga
     1681 tcgagcggct cgagatgggc gtcgtgaagc ttgatgattc cggccatgag ttctttgagc
     1741 aatccggccg aaaggtcgtc ctccggacat tcaagcagcg aacggatttg cctcaagatc
     1801 tcgtcgttcg acaggatcgt gcgagaacgg ctcggtgtgg ttttcatgaa atggcgtcca
     1861 atcggtgcat agtgagtgag agaaaaatct tatcagttga tggtcatttg gtcaaagaag
     1921 ttgcgtcgaa tgcgatcagg gccgatcaag agaggaaggg atcgcttgca gggtccacgc
     1981 tttgaggcga gtcaggtcgc tggccgagag gtcagagaat tggatttcaa cggcgacggt
     2041 tcgttctgct gattcatgtg acgtggtcga tggaagtgtc tctgacgaca agacttttcc
     2101 gtgaatggtc aacggcgtgt gctgcccagg cagggagaac gccaggacga cacgcgtctt
     2161 cgggggcaag ctggaagccg attcgatcct ggcgctcacg tggctcagtt gcacgatggt
     2221 cgcattatgc gcctgtcgtt gaggcaccgc ctcatcgacg atggtgacga cggtgggaat
     2281 atttattaga cgccgcgccg gcgtcagcat cagatcttga gcgctgagca cgccgaccgg
     2341 ctggttctgc ttcgtcacga tcaagagtga cgcgcctgtc gccaccatca tggtggacgc
     2401 ctcttccagc agttcatcat attcgatgaa ttggaccgga cgggtcatga tgctgcgcac
     2461 ttcaatgtcg tgcggttcca ggccctgagc cacgaccttt ttcacaatgt catggggggt
     2521 catcaatcca aagaccgtct cggtttcctt caccagcagg caaggggcat gctcacgttc
     2581 gagcagcagt gccgcttcgc tgacagagac gtctcccggc acttgtacga ctcccggagt
     2641 catcatatgg gctaccacga ttggtgtcgg atgatgggcc tgtggggacg gttcattcgg
     2701 accgagcatc gatagaccct ctccgatcgt gcctcattga cgattcgtga ggggccggcc
     2761 ccctgcggcg taagccaagt atagcagaca gggtttcctg gcccccagcg gctcttccct
     2821 tgtcaaacaa ggggttccat gactagacta ggccaattat tgtctcagca caagtggcga
     2881 ccatgggttg tgaacgctct gtgaatcagg tgtttcagcg tcgtgccgag ga
//
LOCUS       DASTQY010000207         2902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1423451, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000207 DASTQY010000000
VERSION     DASTQY010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423451"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2872)
                     /locus_tag="VFR82_06925"
     CDS             complement(<1..2872)
                     /locus_tag="VFR82_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501368.1"
                     /translation="MVVASTGQPATTTNSDSIAPQGKTVELEWYIPPTQQEGGRQFHS
                     YSHDRELTVMGLFGTFVIEPKGSEYLDPIGTGEPKSMTSGWQAIIKNGAGPDFREFVI
                     IYHEVGDEAFRPLNKKGDFIPQRDPLTDVYRPVGRALNYRSEPFGVDNMQTQHEYFAF
                     EDESMAYSAYTFGDPATTVPRSYLGDPAKFRLVHGGSEVFHSHHPHGGSIRWPRSPRA
                     IDEMPLWHAAKNGPVKYPVIRMKSDRVDVEVIGPSETMDLETECGSGLCQQLAGDFLF
                     HCHVAHHYIAGMWGYWRVYNTLQQGEYHNDVMPDLRELPDRAGRIKNGVTSDQLIGKT
                     VDWFGKQFRIIDKGKSDWKADPAVVTIKDWVEMQLPPQGKPGHTDNEMNQTRAYDASV
                     LNWAWDGHRAMTEPENTVPNPRYQSANPGKRLPIVFESATGKIAWPHLKPHFGRRVPF
                     SQNHNPAPWLDMIHLDEDGLPSSYPAKPGENGRWSMCPENAGSKKYNVHFIQTPMTLT
                     DKQGQTPAIRDKDGLIYVLHEEEEQVRKSDIKYPLVVRANIYDCIDWMLTSEWEDDDH
                     INFQSSKINTHWHFLQFDNQSSDGVITGFSYEQSVRPFTMLEKKVKKGLPLPMNTTFT
                     KAAKKGERVITVKNALQYHPNVELLIGADNVKGNEIGRVKSIKGNEITLYRPLKNDHP
                     VKDIVTVEFVRQRFWVDSDVGTVFWHDHALGRVTWPHGGFGTIVIEPVGSTYHDPKTG
                     KQVRSGPLVDIRTAEPVGFGVNGSFRELVVQLNDTVPHTVNIVTEGNPPGQPIEVALE
                     AGKTVSFPMPEKIPMSPMAFLNGGTHTTGGGLNFKVEPISSRLAVNPDTSKLFSSEVH
                     GDPYTPMVRAYLGDTVVFRLLQTMANETMVWTLSGHTYLTERYAGDANRKNSIHIGIA
                     ERYDLVVPQAGGPRLQPGDYIHLNGRVSKFSEGAWGIMRVLDK"
BASE COUNT          566 a          844 c          801 g          691 t
ORIGIN      
        1 ccttgtccaa cacacgcatg atgccccaag caccttccga gaacttcgac acccgaccgt
       61 tcaaatggat gtagtcgccg ggctgcagcc gcgggccccc tgcctgtggc acgacgaggt
      121 cgtaccgttc ggcaatgccg atgtggatgg aatttttccg attggcatca ccggcatacc
      181 gctcggtgag ataggtatgg cctgacagcg tccagaccat cgtctcgttg gccatggtct
      241 ggagcagacg aaatacgaca gtgtcgccca agtaggcgcg caccattggc gtgtagggat
      301 cgccgtgcac ctcgctgctg aacaacttgg acgtatccgg gttgaccgcc aggcggctcg
      361 agatcggctc gaccttgaag ttcaacccgc ctccggtggt atgggtccct ccgtttagaa
      421 aggccatcgg cgacatcgga atcttttccg gcatggggaa cgacaccgtc tttcccgcct
      481 cgagcgcaac ttcaatcggc tgtccgggtg ggttgccttc cgtgacaatg ttcacggtat
      541 gcggcacggt atcgttgagc tgtacgacca actcgcggaa actgccgttc acgccaaacc
      601 cgaccggctc tgcggtccgg atgtcgacga gtggcccgct cctgacttgc ttgcccgttt
      661 tgggatcgtg gtacgtcgag ccgacaggct cgatcacgat cgtgccgaag ccaccgtgcg
      721 gccatgtcac gcgtccgagc gcatgatcgt gccagaacac cgtcccgaca tctgagtcca
      781 cccagaaccg ttgacgaacg aattccaccg tcacgatgtc tttcacggga tgatcattct
      841 tcagcggccg gtagagcgtg atttcattgc ccttgatgga cttgactcgg ccgatttcgt
      901 tacctttgac attatcggca ccgatgagga gctccacgtt cggatggtat tgcaaggcat
      961 tttttacggt gatgacccgc tcgccttttt tggccgcctt ggtgaacgtg gtattcatcg
     1021 gcaacggcag accttttttc accttctttt ccagcatggt gaaggggcgt acggattgtt
     1081 cataagagaa cccggtgatc acgccatccg atgactgatt gtcgaactgc aggaaatgcc
     1141 agtgcgtgtt gatcttcgac gattggaaat tgatatggtc gtcatcttcc cattcgctgg
     1201 tcagcatcca gtcgatgcaa tcgtaaatat ttgcccggac taccagcgga tacttgatgt
     1261 cgcttttccg cacctgctct tcttcttcgt gcagcacata gatgaggccg tccttgtccc
     1321 tgatagccgg cgtctgacct tgcttgtcgg tcaacgtcat tggagtttgg atgaaatgca
     1381 cgttgtactt cttcgagccg gcattttccg gacacatgct ccaccgtccg ttttcccctg
     1441 gtttggcagg ataggaactg ggcagtccgt cctcatccaa atggatcata tccagccagg
     1501 gagccggatt gtggttctgt gaaaacggca ctcggcgtcc gaaatgcggc tttaaatgcg
     1561 gccaggcaat cttgccggtg gcgctttcga agacgatcgg tagccgcttg ccagggttcg
     1621 cggactgata acggggattg ggaaccgtat tctccggctc ggtcatggcg cgatgaccgt
     1681 cccacgccca attcaggacg ctggcatcgt aggcgcgcgt ctgattcatt tcgttatccg
     1741 tgtggcccgg ctttccttga ggaggcagct gcatttcaac ccaatccttg atcgtcacca
     1801 ctgccggatc ggctttccaa tccgacttcc ccttgtcgat gatgcggaac tgcttcccga
     1861 accagtccac ggtcttgccg atcaactgat cagaggttac cccgttttta atgcggccgg
     1921 cgcggtcggg caattcgcga agatccggca tgacgtcgtt gtggtattca ccctgctgca
     1981 gcgtgttgta cacccgccaa tatccccaca tgcccgcgat atagtgatgc gcgacgtgac
     2041 agtgaaacag gaaatcgccc gcaagctgtt ggcacaaacc ggacccgcac tcggtctcga
     2101 gatccatggt ctctgacgga ccgatgacct ccacatcgac acgatcggac ttcatccgaa
     2161 tgacaggata tttgacagga ccgttcttcg cggcatgcca taatggcatt tcatcgatgg
     2221 cccgcgggct ccggggccaa cgaatggatc caccgtgagg atggtgcgaa tgaaagactt
     2281 ctgacccgcc gtgcacaaga cggaatttgg cgggatcgcc caggtagctt cgcggcactg
     2341 tggtcgccgg gtctccaaac gtataggcgc tgtacgccat cgattcgtct tcaaacgcaa
     2401 agtactcatg ctgcgtttgc atattgtcga cgccgaaggg ctcgctgcgg taattcagtg
     2461 cccgtcccac cggcctgtag acgtcggtca agggatcgcg ttgaggaatg aaatcgcctt
     2521 tcttattgag tgggcgaaat gcctcatccc ccacttcatg gtaaatgatg acaaactcgc
     2581 ggaagtccgg acctgcgccg ttcttaatga tcgcctgcca tccgctggtc atcgactttg
     2641 gctcgcccgt gccgatggga tccaaatatt cggagccttt tggttcgatc acaaaggtgc
     2701 cgaacagacc catcacagtt agttcccgat cgtgactgta agaatggaac tgccgcccgc
     2761 cctcttgttg cgtcggcgga atgtaccatt ccaactctac ggttttccct tgcggggcga
     2821 tgctgtcgga attcgtcgtc gtcgcaggct gcccggtaga tgccaccacc atgctggagc
     2881 cgtgtatgtt gaggctgact tc
//
LOCUS       DASTQY010000208         9642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_544783, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000208 DASTQY010000000
VERSION     DASTQY010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9642
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544783"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..2617)
                     /gene="clpB"
                     /locus_tag="VFR82_06930"
     CDS             complement(20..2617)
                     /gene="clpB"
                     /locus_tag="VFR82_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEU4501369.1"
                     /translation="MDMNRMTIKLQEALQTASAHAMRRSHQGMDVEHLLLAFLEQEGG
                     IVPALCEQAGISVPAVRQAAEQALSKLPQVQGTGGGPGQLHLTSRLNQVLTKAEDEQR
                     TLKDDYLSVEHVLLAMVHEGGIFKKLGLSKDKLLAALQQVRGHQRVTSQDPEGTYQSL
                     EKYGRDLTKLAGQGKLDPVIGRDDEIRRVIQILSRRTKNNPVLIGEPGVGKTAIVEGL
                     AIRIVKGDVPEGLKQKRVVALDMGALVAGAKFRGEFEERLKAVLKEIQASQGQVLLFI
                     DELHTVVGAGAAEGAMDAANLLKPMLARGELHLIGATTLDEYRKHIEKDAALERRFQT
                     VFVDQPSVENTISILRGLKERYEVHHGVRIKDAALVAAAKLSHRYIADRFLPDKAIDL
                     MDEAAARLRTEIDSLPADLDEVSRKVLQLEIEREALRKEKDPVSQARLTVLEKELSER
                     QRDLQTLKTRWDSEKASVARLRKTREAIEEVKLAIEKAERAYDLNRVAELRYGELPRL
                     ERELSIEQQQLGKKQGESRLVKEEVDEDDIAAIVSRWTGIPVSRLMEGETEKLLRLEE
                     LLHQRVVGQDEAVRAVADAVLRARSGIKDPNKPIGSFLFLGPTGVGKTELARALATTL
                     FDDETNMIRIDMSEYMEKHTVARLIGAPPGYVGYEEGGQLTEAVRRRPFSVILFDEIE
                     KAHHDVFNVLLQVLDDGRLTDSQGRTVDFKNTVLIMTSNIGSPQILEAQQKGSSYDDV
                     RTVVMGELRRHFRPEFLNRVDEIVVFHPLETDQLTKIVEIQLERLRGRLAERRITLTM
                     SPGALQHLAARGYDPVYGARPLKRLIQQYLETPMARQLVKGELRDGDTAMVDVKGDGI
                     VIVPTVTKA"
     gene            2737..3567
                     /locus_tag="VFR82_06935"
     CDS             2737..3567
                     /locus_tag="VFR82_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501370.1"
                     /translation="MVNHLVGALDLYRQALRKTWQSLQRGWITILAVIGFLFLLILAQ
                     QIAAPLGIAGGFLLGAVNALLVGVTLSLIEQSISYARTLTIKDVVESFGHYFWDVIGV
                     GFVLWLPIMALDMGTRQNPNGPLISYAVMLLMFILLNPAPEVIYQVRHDSPLDVFKRA
                     YDFVLENWIEWFLPMALLLLPLLAAPDGLRTFFLLSQRVGRGAGLDFSQLLIMPFTIL
                     GSWLASVGIDPELSQYLILFFTPPLAVSILLFRGHLFALLSRSSHRQRQFASQVQGKD
                     "
     gene            3739..5211
                     /locus_tag="VFR82_06940"
     CDS             3739..5211
                     /locus_tag="VFR82_06940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU4501371.1"
                     /translation="MRLSIFWRIVLTSLLIIVVMAGVNFYALFQLRQLSALSTTLASQ
                     HYPAIEQAKHLQAVAYNELNSEKKYLAVRDPTFLQHFDEEVEEFHRGVENLLRQDSSE
                     EGHQFLEQVRRYQQDRLALFHAELENAANAPGSVSPEYESRRNEVMDHMTATLDHYVD
                     YHEGVISAGVSRSRESSAQAEAITEQLVLLALVFGVGLAGIASYTILRPLRQLQGHIK
                     QIGQGNFRASLNIRAPSELRDLVDTVNWMGGKLQELDDMKGEFLAHVSHELRTPMASI
                     QEGTHLLLDEIPGPVTLEQRTTLRIMADSSRRLITLISTILDLSKMDAGMMEYRIVPT
                     DLKRITDMSVNKIRLLADAKHVQLVVEAPAQRVWVKADAIRIEQVLDNLLSNALKFSP
                     EGGIVKTQMKSDHKAGVLEVAVSDVGPGIPADELPHIFERFYQGSTKSRHSTPGSGLG
                     LALAKKVVEAHGGRIWIESEVRKGTTVRFILRLTKAGAAS"
     gene            5208..5987
                     /locus_tag="VFR82_06945"
     CDS             5208..5987
                     /locus_tag="VFR82_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501372.1"
                     /translation="MKAMRMASGGLALILMTGGGCGSWSSPPQLSQPYFVVDPSDAKT
                     FHMIGKKQDAVAAKCQEHNSCDHAYFTRALIGLYESRDVAIRYFEKVIAVAPRGQLAA
                     SSRLWLEQIQQHPAPVERSWFTSVMSAPAISVSQVTLAQTSDRLVRDLLDREMMIQQL
                     RASKDADAQTLDQLQRELADRDRKVDAPAGKKDPGKGGTESTNVQVLQRQLSERDKKI
                     DELSNQLEALKRIDQEMRDKVRPIRPPSTMVPPPSPEPINP"
     gene            6011..7429
                     /locus_tag="VFR82_06950"
     CDS             6011..7429
                     /locus_tag="VFR82_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU4501373.1"
                     /translation="MEQEHILVIDDEEGLLQLVKMRLNAMGFTVTACTSGREAVNAAK
                     TNRFDLAITDLRLRGEDGLDVTEELLRIHPGLPVIILTAHGSIPNAVEAVQRGAFGYL
                     TKPFDDKELKAKIEEGLSPQRMSREIQRLKSLVNELYGMENVVARSPAMQRLLQQVIQ
                     VADSDATMLLFGETGTGKEVFARVTHANSRRSKGPFVALNCAAIPETLFESELFGHVR
                     GAFTSAHGPKKGLFQSANGGTLFLDEIGEMPLSMQVKLLRAVQEREVREVGAEHPTKV
                     DVRIIAATNKDLGEAVKNGSFRNDLYYRISVVPLFIPPLRDRRDDIPILAQYFLTSSA
                     KRANKDVRGFTPAALHRLVTNLWPGNVRELENAIEKAVVMSRQDMITPDLLPSIGTAP
                     DSQLKPLTEAKEEFEKTYLKNVLQLTGGNISRAAQFAGRYRADFYKMLKKYGLHPSTN
                     KGKPGPGIEDLEEEDHLTEAER"
     gene            complement(7575..8885)
                     /locus_tag="VFR82_06955"
     CDS             complement(7575..8885)
                     /locus_tag="VFR82_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501374.1"
                     /translation="MNDHRLRWTLAVITAAATGALYAEGVLISGWIEVVLCIPIAILL
                     FGTRSESSLDRHLQNGLITITAVSLSLVCLDLILRPTFGHKLHYTPTNITSHKMPELP
                     IVGRWDPNLTIDAEGYGDLAAMAGDPAFQERRQIVFRTDQYGFRNIPRSDTTDLLILG
                     DSFPAGGGTSDEEIFARLLETRYGFHTYNLSFPGGPYDQFINFAIEWRRLNVASHPRM
                     IWTFYTGNDMDDDGGEVWDISALPWKQGFSAWRVKFKSYRNRSPLRQWIEGIRMKLRG
                     WPKYVITRSLPDGHPVLFLENQERWGKASRHDVEQHPNYKKLLRTMTAMQKLTAERHV
                     DVTGLILPTKGEVYRWLLEARDSQPDDESASGFAEAVLEACRTTGIVCHDTKPYLISE
                     AKRLYKQEGKLLWWRDDTHIGKYGHAAIAAFIAGSVLNKHAMVQ"
     gene            9274..9534
                     /locus_tag="VFR82_06960"
     CDS             9274..9534
                     /locus_tag="VFR82_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501375.1"
                     /translation="MESTNAGYALQFDKHTCSVIENMKPGDERTVLVVPEINSKGEPK
                     AARARRSQDGEEFVVIMPDGVEAKWTYDDLKSTIIPHYKPLA"
BASE COUNT         2122 a         2725 c         2620 g         2175 t
ORIGIN      
        1 gtgggtgggt aagtctaggt catgccttgg tcaccgtcgg cacgatgacg atgccgtcgc
       61 cctttacgtc caccatcgcc gtatctccgt cccgcaactc gcctttcacc aattgtctgg
      121 ccatcggagt ttcgaggtac tgctgaatca gccgctttag cggccgggcg ccgtacacag
      181 gatcatatcc gcgtgctgcc agatgttgaa gcgcaccggg cgacatcgtg agcgtgatgc
      241 gtcgttcggc cagtcgtccc cgcaaccgtt ccaattggat ttccacgatc ttcgtcaact
      301 ggtccgtctc cagcggatgg aacacgacga tctcgtccac gcgattcaag aactcaggac
      361 gaaaatgccg gcgtaattcg cccatgacga ctgtgcggac gtcgtcgtag ctggaacctt
      421 tctgctgcgc ctcgaggatt tgcggacttc cgatgttgga cgtcatgatc agcaccgtgt
      481 ttttgaagtc cacggtccgt ccttgagaat cggtaaggcg tccgtcgtcc aagacctgaa
      541 gcagcacgtt gaagacgtcg tgatgggcct tctcgatttc atcgaagagg ataacggaga
      601 aggggcgccg ccgcaccgcc tcggtcaact gcccgccttc ctcataaccg acatagccgg
      661 gcggagctcc gatgagccgg gcgaccgtgt gcttctccat gtactccgac atatcgattc
      721 gaatcatgtt ggtttcgtca tcgaacagcg tcgtagcgag cgctctggcc aattcagtct
      781 tgccgacgcc cgtcggcccc agaaagagga aggagccgat tggcttgttg ggatccttga
      841 tgcctgatcg cgcgcgcagg acggcgtcgg ccacggcccg aaccgcctcg tcctgaccga
      901 ccacccgttg atggagcaac tcttccaacc taagaagttt ttccgtctcg ccctccatca
      961 accgcgagac cgggatgcca gtccaacggc tcacgatggc agcgatatcg tcttcatcca
     1021 cctcttcctt gaccaagcgg ctctctccct gttttttccc caactgctgt tgctcgatcg
     1081 acaattcacg ttcgagacgt ggcagttcac cgtaccgaag ttcggccact cggttgagat
     1141 cgtatgcgcg ctctgccttc tcgatcgcca gcttgacctc ttcgatggct tcacgtgttt
     1201 ttctgagccg tgccaccgag gctttctcgg aatcccatct ggtcttcagg gtttgcaagt
     1261 cgcgttgccg ttcactcagc tctttctcga gcaccgtcaa ccgcgcctga ctgaccggat
     1321 ctttttcctt gcgtaatgct tcgcgctcga tttcgagctg cagcaccttg cgcgacacct
     1381 cgtcaagatc ggcggggagg ctgtcgattt ccgtccgaag gcgcgccgcg gcttcatcca
     1441 taagatcgat cgccttgtca ggcaggaacc gatcggcaat gtagcgatgc gagagcttcg
     1501 ccgccgccac gagcgcagca tccttgatcc gcacgccatg atgcacttcg taccgttcct
     1561 tcaggcctcg caggatggaa atcgtattct cgacggaggg ttgatctacg aacacggttt
     1621 gaaaccgccg ctccagcgcc gcatcctttt cgatatgttt gcggtactcg tcgagcgtcg
     1681 ttgcgccgat cagatgcaat tcgccgcggg ctagcatggg cttgagcagg ttcgcggcat
     1741 ccatggcacc ctcagcggct cctgccccga cgaccgtatg caattcatcg ataaacagca
     1801 gtacctggcc ctgagaggct tgaatctctt tcaagacggc tttcaaccgc tcttcaaact
     1861 cgccgcggaa ttttgccccg gcgaccaacg cgcccatgtc gagagctacc acgcgtttct
     1921 gcttcaatcc ttccggtacg tctcccttca cgattcggat ggcgagccct tcgacgatgg
     1981 cggttttgcc gacgccgggc tccccaatga ggacgggatt gttttttgtt cgacgggaca
     2041 gaatctggat gacacggcga atctcgtcgt cgcgcccgat cacgggatcc agtttgccct
     2101 ggccggccag tttggtgagg tcccggccgt acttctcgag cgattgatac gtcccttccg
     2161 gatcctgact ggtgacgcgc tgatggccac ggacctgctg caaggccgca aggagcttgt
     2221 ctttggacaa tccgagtttc ttgaaaattc cgccttcatg caccatggcg agcagcacat
     2281 gctccacgct gagatagtcg tctttcagcg ttcgttgttc gtcctctgct ttagtaagca
     2341 cctgattgag acgtgaggta aggtgaagct gccccgggcc gccgcccgta ccctgcacct
     2401 gcggcaattt gctcaaggct tgctccgccg cctggcggac agcggggacc gaaatcccgg
     2461 cttgctcgca tagggcgggc acgataccgc cttcctgttc gagaaaagcc aggaggagat
     2521 gctcgacatc catgccttga tgactgcggc gcatagcgtg ggccgatgca gtctgcaggg
     2581 cttcctgtag tttaatggtc atccggttca tatccatgat gatggctcca gattcagtct
     2641 actgactcgt ataccgacat aatcacaagg agtcgaaagt caacttggga cggtgactgc
     2701 cttgaccggc cggacgggac cagactattg tcattcatgg tcaatcacct tgtgggggct
     2761 ttggacctct atcgccaggc gctgcgtaag acgtggcaat ccctgcagcg aggttggatc
     2821 acaattctgg cagtcatcgg attcttgttt ctcctcatct tggcccaaca aatcgcagct
     2881 cctctgggaa ttgcgggcgg ctttctgctg ggcgccgtga atgccttgct cgtcggggtg
     2941 accttgagtc tgatcgagca gtcgatttcc tatgctcgga cgttgacgat caaagacgtc
     3001 gtcgagagtt ttggccatta tttttgggat gtcattggcg tgggcttcgt cctctggctt
     3061 cccatcatgg cactcgacat gggcaccagg cagaatccga acggtcccct gatttcctat
     3121 gccgtgatgc tgttgatgtt cattctgttg aatcctgctc ctgaagtcat ctatcaggtg
     3181 cgtcatgatt caccgctgga tgtgttcaag cgggcctatg actttgtctt agaaaactgg
     3241 attgaatggt tccttccgat ggccctgttg ctgctacccc tcctggcagc ccctgacgga
     3301 ctgagaacct tctttctgtt gtcccagcga gtaggcagag gcgcaggtct cgacttctcc
     3361 caacttctca tcatgccctt tacgattctg gggagctggt tggcttctgt gggaattgat
     3421 cctgaactgt ctcaatacct catccttttt ttcactcccc ctctcgctgt gtcgatcctc
     3481 ctgtttcgtg gccacctctt tgcattactg tctcggtctt cccatcgcca gcgtcaattt
     3541 gcatcgcagg ttcaagggaa ggactaggac gtaactcttg acggcctacc ccggaatctc
     3601 aatgtcttat aggtaagagg acggggttca tcgctggtgg acccatgttc gctcttgagt
     3661 gacaagtgaa tttagtgtat ggatttacct acacacagag ccccctcgtt tctcgataca
     3721 tcaaagggag ctgcgctcgt gcgtctgtca attttctggc gcattgtcct cacatcactg
     3781 ctcatcattg tcgtgatggc cggagtgaac ttctatgcgc tgtttcagct tcgtcaactg
     3841 tccgcattga gcacaacgct cgcctctcaa cattacccag ccatcgagca ggccaagcat
     3901 ctccaagcgg tggcctacaa cgagctcaac agcgagaaaa aatacctggc tgtccgtgat
     3961 ccgacatttc ttcagcactt tgacgaagag gtcgaggaat tccaccgagg cgttgagaat
     4021 ttattacgcc aggattcatc ggaggaaggc catcaattcc tcgagcaggt gcggcgctat
     4081 caacaagacc ggctcgccct gtttcatgcc gaactggaaa atgccgccaa cgcgccgggt
     4141 tccgtgtctc ccgagtatga gagccgccgc aatgaagtca tggatcatat gacagccacg
     4201 ctggatcact atgtggatta tcacgaaggg gtcatcagcg ccggggtcag ccgatcccgc
     4261 gaaagttcgg cgcaggccga agcgatcacc gagcagctgg tcttgctcgc actcgtcttc
     4321 ggagtcggct tggccggaat cgccagctat accatccttc gtccgctacg ccagctgcaa
     4381 ggccacatca aacaaatcgg acagggtaat ttccgggcgt ccttgaacat ccgcgcaccg
     4441 agcgaactcc gagatttggt ggacacggtc aactggatgg ggggaaagct ccaagagctg
     4501 gacgacatga agggcgaatt cctggcccac gtttcacacg agctgcgcac tcccatggct
     4561 tccatccagg aagggacgca cctgctcctc gatgaaattc ctggcccagt gacgctggag
     4621 caacggacga cgctccgcat catggccgac agtagccgac gcctgatcac gctgatctca
     4681 accatcctgg atctctccaa aatggacgca ggcatgatgg aatatcgcat cgtgccgacc
     4741 gatctgaaac ggatcaccga catgtccgtc aataaaattc gtctcttggc cgacgcgaaa
     4801 cacgtgcaac tcgtcgtcga agcaccggcg caacgcgtgt gggtgaaggc cgacgcgatt
     4861 cgtattgagc aggtcttgga taatttgcta tctaatgcct tgaaattcag tcccgaagga
     4921 ggcattgtca aaacgcagat gaaatcggac cacaaggcgg gtgtccttga ggtcgcggtc
     4981 tccgacgtcg ggcctggaat tcccgcagac gagctgccac atattttcga gcgcttttat
     5041 cagggcagta cgaaatcccg acattctacg ccaggaagcg ggttgggatt ggccttggcg
     5101 aaaaaagtcg tcgaagccca cggaggcagg atttggatcg aaagcgaagt gaggaaaggc
     5161 acgactgtcc ggtttatcct gcgcctcacc aaagcgggtg cggcctcatg aaggccatga
     5221 ggatggcgag cggtggactg gcgctgatcc tcatgacggg aggaggttgt ggttcgtggt
     5281 cctctccccc ccagctgtca caaccgtatt tcgtcgtgga cccatcggat gccaaaactt
     5341 tccacatgat tgggaagaaa caggatgcgg tcgctgcaaa gtgccaggag cacaattcgt
     5401 gcgaccatgc gtattttacc cgtgctttga taggcctgta tgaaagccgg gacgtggcta
     5461 tacgctactt tgaaaaggtg attgccgtgg cgccgagagg ccaattggcc gcatcaagcc
     5521 ggctgtggct cgagcaaata cagcagcatc cagcccctgt ggaacggtcc tggtttacct
     5581 cggtcatgtc ggcaccagcc atatcggtca gccaagtcac gctggcacaa acatctgatc
     5641 gtctggtgcg ggacttgctt gatcgggaga tgatgattca gcagcttcgc gccagcaagg
     5701 acgccgatgc ccagacactg gaccagctcc agcgggaact ggccgatcgt gaccggaaag
     5761 ttgatgcccc ggcgggcaag aaggatccag gaaagggggg aaccgagagt acgaacgttc
     5821 aggtgctcca aaggcagcta tcggaacggg ataaaaagat cgacgagctc tcaaaccagc
     5881 tggaagccct caaacggatc gatcaggaaa tgcgcgataa agttcgcccg atccgtccgc
     5941 cgtccaccat ggtgccaccc ccgtcaccag agccaatcaa tccataacgt acaaaaggat
     6001 cagcactgtc atggaacaag agcacatcct ggtcatcgac gacgaagaag gtctcttaca
     6061 gctagtcaag atgcggttga acgcgatggg ctttaccgtc accgcttgca cttccggccg
     6121 agaagccgtc aatgcggcaa aaaccaatcg attcgacttg gccatcactg acctccgcct
     6181 gcgcggggaa gacggattgg atgtgacaga agaattgttg cgcatccatc ccgggctgcc
     6241 cgtcatcatt ttgaccgctc acggaagcat tcccaatgca gtcgaagcgg tgcagcgggg
     6301 agccttcggc tatttgacaa agccatttga cgacaaggag ctcaaagcca agatcgagga
     6361 gggtctgtcg ccacagcgaa tgagccgcga gatacaacgg cttaaatctc tcgtcaatga
     6421 gctctatggc atggagaacg tcgtcgcacg gagtcctgcc atgcagcgcc tgttgcagca
     6481 ggttattcag gtcgcggatt cagacgccac tatgctgctg ttcggagaga cgggaacggg
     6541 caaagaggtg tttgcccgcg tcacgcacgc caacagtcga agaagcaagg gccctttcgt
     6601 cgcgttgaac tgtgcggcca ttccagagac gctcttcgag tccgagctgt tcggtcatgt
     6661 tcgcggcgcc ttcaccagcg cgcacggtcc caagaaaggt ttgttccaaa gtgcgaacgg
     6721 aggcacactg tttctagatg aaatcggaga gatgccgctg tctatgcagg taaaactcct
     6781 gcgcgctgtg caggagcgag aagtgcggga agtcggtgcc gaacacccga ccaaagtcga
     6841 cgtgcggatc atcgcggcga ccaataagga cttgggcgaa gccgtaaaaa atgggagctt
     6901 ccgcaacgat ctgtattacc gcatctcggt cgtgccgttg ttcattccgc cgcttcgaga
     6961 ccgccgcgac gacattccga tccttgccca gtacttctta accagcagtg cgaaacgggc
     7021 caataaagac gtgcgcggtt tcacgccggc cgcgttacac cggctggtga ccaatttgtg
     7081 gcctggaaat gtccgcgaac tcgagaatgc catcgaaaaa gccgtcgtca tgtcgagaca
     7141 ggacatgatt acgcctgatc tgctgccgtc catcggcacg gctccggact cgcaactgaa
     7201 gcctctcacc gaagcgaagg aagaatttga gaagacctat ctgaagaatg ttctgcaatt
     7261 gacaggcggc aatatttcac gcgctgccca attcgccggg cgctatcgag cggacttcta
     7321 caaaatgctc aagaagtacg ggttgcatcc ttcgaccaac aagggcaagc ccggacccgg
     7381 aatcgaagat ctcgaagagg aagatcatct cacggaggca gagcggtaac ccgccgtcac
     7441 tcacgccgtc cgcccaggcg tcgttccctg ttgattctca tcctaacacc gtttccggtg
     7501 acttgcgatc acaccgtata tcttacgaaa gactgcactt tcatcagtca atgcagctat
     7561 cgtgatagca caatttattg aaccatcgca tgcttgttca gcacgcttcc agcaataaag
     7621 gccgcgatcg ctgcatgacc atatttccca atatgcgtat cgtctcgcca ccacaagagc
     7681 ttgccttctt gcttgtatag tcgctttgcc tctgagatta gataaggttt cgtatcgtgg
     7741 cacacgattc ccgtcgttct gcacgcttca aggactgcct cagcaaaacc cgaagcgctt
     7801 tcgtcgtcag gttgcgaatc acgggcctct aacagccaac gatagacctc gccctttgtg
     7861 ggaagaatca agccggtgac gtcgacgtga cgttcagctg tgagcttttg cattgcggtc
     7921 atcgtccgta acaacttctt gtagttggga tgttgctcga cgtcatgacg ggatgctttc
     7981 ccccatcttt cttgattttc taggaacagg acgggatgac catccggtag tgaacgagta
     8041 atcacatact tgggccatcc acgtagcttc atgcgtatac cttcgatcca ttgcctgagt
     8101 ggcgagcgat ttctatagct tttaaacttc acccgccaag cagagaagcc ttgtttccac
     8161 gggagagctg agatgtccca gacctcacct ccgtcatcgt ccatatcgtt ccccgtgtag
     8221 aaagtccaga tcatccgagg atgggaggcg acattcaatc ttctccactc gatggcgaaa
     8281 ttgatgaact gatcatacgg tccaccaggg aaggaaagat tgtaggtgtg gaagccatac
     8341 cttgtctcca acaagcgagc gaagatttcc tcgtcgcttg ttccgccccc agcaggaaaa
     8401 gagtcgccca gtatcaacag atccgtcgta tccgagcggg gaatgttccg aaagccgtat
     8461 tgatctgtac gaaacacaat ttggcgacgc tcttggaaag ccggatcacc ggccatggcg
     8521 gccaaatcac cgtagccttc ggcgtctatg gtgagattgg ggtcccaccg accgacaatg
     8581 ggcagttctg gcattttatg ggatgtgata ttggtgggag tgtaatgcaa tttatgacca
     8641 aaggttggtc gcaggataag atccaaacac acgagcgaga ggctgactgc cgtaatggtg
     8701 atgagcccgt tttgtaggtg acgatccagt gatgactctg accgcgtccc aaacaataaa
     8761 atggcgatcg gaatacagag cacgacctcg atccaccctg aaatgagaac cccctcagca
     8821 taaagagccc ccgtcgcggc agcagtaatc actgccaagg tccaacgtag tcggtggtca
     8881 ttcacaccat gctccttcac gctcgatgtt gctggaggtt gtgatttggt cggggacatg
     8941 caggttacag gacaacgcca gctttgcgca acagcaacta gcacacgttg cgcgaattga
     9001 ccctcaaggt cggtttctag cagaaagatg acaggagaag cggcccgcag cgcgacgtgc
     9061 catgaagatc tcagaatgaa agcgtggccc cgctgggcta ggaccgtcct gcctagccaa
     9121 tcgtaggtat gtgctcgttg cagctggcac gaaggcaaga ggtaagactg gcgcgcaggt
     9181 ggtccagttt tgattgcttg accttttctg cgccattggt aagactaaga agacctaaaa
     9241 ctagacattc gaatctggct gggaggcact ggaatggaat cgacaaatgc gggctatgct
     9301 ctgcaatttg acaagcacac atgttcggta attgagaata tgaaacctgg tgacgaacgg
     9361 acggttctgg ttgttccaga aatcaattca aagggcgaac ctaaagcagc tagggcaaga
     9421 aggagtcagg acggggaaga gttcgtcgtc atcatgccgg atggtgttga ggccaagtgg
     9481 acttacgatg acctcaagag cacgatcatc ccacactata aaccattggc ctaaagatac
     9541 cctccggtca gtgtgtttga taagcagcgc acactcatga atttgagtag cgatttacct
     9601 tcgtaaacat tcaaccgcat gggttctcta gccgcctacc gt
//
LOCUS       DASTQY010000209         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_23373, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000209 DASTQY010000000
VERSION     DASTQY010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23373"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VFR82_06965"
     CDS             <1..762
                     /locus_tag="VFR82_06965"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEU4501376.1"
                     /translation="LFGYLYGYTLFDWALFAILYVCTGLGITVGYHRLISHRSFECHP
                     WVKALLLIAGGWALQNSALRWSADHLRHHARTDQEEDPYNAQKGFWHSHCIWLFINTS
                     HRTENYERQLRKDPIVMWQHRHYHAVVISGLAFPLLLGSLHGGLIGGIGCFFLAGMFR
                     MFMVLNSTFAINSLCHMFGTQPHGTGDSSRDNWLVSFVSFGEGYHNYHHTYARDYRNG
                     PRWYNFDPSKWTIYSLSMLGLATNLRRLDSTVMSE"
     gene            974..1837
                     /locus_tag="VFR82_06970"
     CDS             974..1837
                     /locus_tag="VFR82_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEU4501377.1"
                     /translation="MADEGQAAQPKENLIPGVQHVLAISSGKGGVGKSTVAANLACAL
                     ALTGAKVGLMDADLYGPNIPMMMGSTTGPEQRDGKIVPVENHRVKLISMAYLVPEEAP
                     LVWRGPMVHQYLQAFFRDVLWGDLDYLLIDLPPGTGDVQLSLSQMVPLAGAITVTTPQ
                     EVALYDVRKGMAMFQKVNVPLLGIIENMSFFVCGHCGERTDIFSHGGGERAASKLGVP
                     FLGSIPIDPIIREGGDTGHPIVVADPGSPQAAAFREIARKIMESLAGEAKGGASIDSL
                     LKKIKQPFTNQ"
     gene            1869..2174
                     /locus_tag="VFR82_06975"
     CDS             1869..2174
                     /locus_tag="VFR82_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEU4501378.1"
                     /translation="MGEFVRVAAIDDVKPGQGLVAEVNGKALAVFNIDGHIHVIDNTC
                     AHRGGPLGEGELEASVVTCPWHGWQFDVTTGACVNNPSARVQTYPVKVEGSDVKVQF"
     gene            2201..2566
                     /locus_tag="VFR82_06980"
     CDS             2201..2566
                     /locus_tag="VFR82_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501379.1"
                     /translation="MPATAKDQIEIAAALVRLYVFLSQYLDRCVDDVVRTSYPDAELE
                     AHLTETRRTLMSILSVNPVVKRKLTEECDRILALGTSCVKAGTGNAQLHERLVAERNV
                     LKQKTLALSDLVAVFRALD"
BASE COUNT          564 a          754 c          746 g          562 t
ORIGIN      
        1 cttttcggct acctgtatgg ctatacgctg tttgactggg ctctcttcgc catcctctac
       61 gtgtgtacgg gcctgggcat tacggtcggc tatcatcggt tgatttcaca tcgcagcttc
      121 gagtgccatc cctgggtgaa agcgctgctg ttaatcgccg gcggatgggc actgcaaaat
      181 tcggccctcc gctggagtgc cgatcatctt cgccatcacg cgaggacgga ccaggaagaa
      241 gacccatata atgcccagaa aggcttttgg catagccact gcatttggct ctttatcaac
      301 acctcgcatc ggacggaaaa ctacgaacgg caattgcgca aggatcccat cgtcatgtgg
      361 cagcaccggc attatcatgc ggtcgtgatc tccgggttag cgtttccctt gctgctcggc
      421 tccctccacg gcggactgat cggcgggatc ggatgttttt ttctcgctgg aatgttccgg
      481 atgttcatgg tgctcaattc gacctttgcc atcaattccc tctgtcatat gttcgggacg
      541 caaccgcacg ggaccgggga cagcagccgc gacaactggc tggtctcttt tgtcagcttc
      601 ggtgaggggt accacaatta ccaccacacc tacgcgcgcg attaccgaaa tggccctcgc
      661 tggtacaact ttgatccgtc caagtggacg atttactccc tctccatgct cggactggcg
      721 accaatcttc gccgccttga ttcaaccgtc atgtccgaat aagggcctcg agcggtcctg
      781 ccagggccaa tgggcccatt tccttccggc ttctgtatcc cttatgatgc agtagtatgg
      841 agtgatcggt agaccctcgg attctcaaca cgcgaacaag caagagagcc atctccagtc
      901 cgactcatga cgacaggagc acacccgcaa ttgtacggca cagcgtgtga catcgcgcga
      961 caaggagtcc tgtatggctg acgaagggca agctgcccag cccaaagaaa acctgatccc
     1021 gggcgtccaa catgtcctcg ccatcagcag cgggaagggc ggcgtcggta aatcgacagt
     1081 tgccgcgaac ctcgcctgtg cgctggctct gacgggcgca aaggtcggcc tgatggacgc
     1141 cgatctctat ggaccgaaca ttcccatgat gatgggcagt acgaccgggc ctgagcaacg
     1201 agacgggaag attgttccgg tagagaacca tcgcgtcaaa ctcatctcga tggcgtatct
     1261 cgtccctgaa gaagcgccgc ttgtgtggcg tggcccgatg gtgcatcaat atctccaagc
     1321 gtttttccgc gacgtgctct ggggagacct cgactatctc ctgatcgatc tgccgccggg
     1381 aaccggcgat gtccaactgt ccttgtccca gatggtaccg ttggccgggg cgattacagt
     1441 caccacgcct caggaagtcg ctctctacga tgtccgcaag ggcatggcca tgttccaaaa
     1501 ggtcaatgtg ccgctgttgg gcattatcga aaacatgagc ttttttgtgt gcggccactg
     1561 cggcgagcgg acggacattt tctctcacgg aggaggagag cgcgccgcat ccaaactagg
     1621 agtcccattt ctagggagca ttccaattga ccctatcatt cgggaaggcg gggacacggg
     1681 gcatccaatc gtcgtcgctg accccggctc tccccaggct gccgccttcc gggagatcgc
     1741 cagaaagatc atggagtcat tggcgggtga ggcaaagggc ggcgcctcga tcgacagctt
     1801 gctcaaaaag atcaagcaac catttacgaa tcagtgacga ctggtcacga ggagggaggc
     1861 agggccccat gggagagttc gttcgcgttg ctgcaatcga cgatgtgaag ccgggacagg
     1921 gcttggtggc ggaggtcaac ggaaaagcac tggccgtctt caatatcgac gggcacattc
     1981 atgtcataga caatacctgc gcccaccgtg gaggtcccct tggtgaagga gagcttgaag
     2041 cgtctgtcgt gacctgccca tggcatggat ggcaattcga tgtgaccacg ggcgcctgcg
     2101 tgaacaaccc gtccgcccga gtccagactt acccggtcaa ggtcgagggc tctgatgtaa
     2161 aagtccaatt ttagcagttc ctcgtgaaca caggacctcg atgcctgcca ccgcaaaaga
     2221 ccagattgaa atcgccgccg ctctcgtgcg tctctatgtc tttctctcac aatacctcga
     2281 tcgctgcgta gacgacgtgg tgcgaacaag ctatccggac gccgaacttg aggctcattt
     2341 gacggaaact cgccgcacat tgatgagcat cctttccgtc aatccagtcg ttaagaggaa
     2401 attgaccgaa gaatgtgatc gaatccttgc cctgggcacc tcgtgcgtga aagccggcac
     2461 tggcaatgcc caattgcacg aaagactggt cgccgagcgc aatgtgctca agcagaagac
     2521 cctcgcgctg agcgatctgg ttgccgtctt ccgggcgctg gattagcggg aatcgtatgc
     2581 acagcaggct gttgagaagg gctgcagggg accgccgcag gagaaa
//
LOCUS       DASTQY010000210         3607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_426260, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000210 DASTQY010000000
VERSION     DASTQY010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426260"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..643
                     /locus_tag="VFR82_06985"
     CDS             212..643
                     /locus_tag="VFR82_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4501380.1"
                     /translation="MSQVSRRFNRKSTRTDVIRVLLVDDVESVRETLRSILATYPNFE
                     VVGEACNGEEALESVQLLKPSVVVMDINMPRLDGIKATTLIKRVYPHIVVVGLSVYAN
                     DDTRKAMTAAGATSVISKEAAVEQLRDEIIESVNRRSAAIH"
     gene            1087..1947
                     /locus_tag="VFR82_06990"
     CDS             1087..1947
                     /locus_tag="VFR82_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU4501381.1"
                     /translation="MGNPGLIPRALALFVAGALTVAAEPSPFAKEAQPPLVETVKGKD
                     GAPMILIPAGPFAMGSNEGLPNERPEHIVELDAYYIDQYEVTLALYRKFLDAGKHDSP
                     PTWDDEAATTVGDRPAIGMKWADADAYCKWAGKRLPTEAEWEKAARGTDARRYPWGSM
                     QPFVDIANYNRGLWVSEAVTLAAVTSGLEGMSVRHGLKEGGKSPYGLNHMAGNAAEWV
                     ADWYDRDYYQNSPKKSPTGPAQGEKRVLRGGSWADLPLALRVTARISAEPEFEDRTIG
                     FRCALPATGQ"
     gene            2029..2511
                     /locus_tag="VFR82_06995"
     CDS             2029..2511
                     /locus_tag="VFR82_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501382.1"
                     /translation="MSDRKVNVLVMGILLIGLVWAYFSARNPAVQTARPVSLLAPEMV
                     PLVSGEEPIATIFTRAGCPVCHQIPGIPGAEGRVGPPLLLGTFGPQRLRDPTYGGRAK
                     TVHEYVVESVLEPGVFVVPGYPPQTMPTWYGAKLSALALEKIATYLEQQMTAKDTQQQ
                     "
     gene            complement(2539..>3607)
                     /locus_tag="VFR82_07000"
     CDS             complement(2539..>3607)
                     /locus_tag="VFR82_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEU4501383.1"
                     /translation="ANTPPFTNEQLEALALALMRGQQRSEFEEKMEMNLALYYKELGR
                     FRVNIFRQRGNVGLVFRHIKAEIQTVEQLRLPQIMKDIAMTKRGLVLVVGATGSGKST
                     TLAAMIDHRNSVHPGHIVTVEDPIEFVHNHKKSLITQREVGFDTLSFQNALKNTLRQA
                     PDVILIGEIRDTETMEAAITFAETGHLCIGTLHSNNANQAIERIMNFFPVERHAQIYL
                     QLSLNLRAIVSQRLVPSTDGKRIPALEIMMDTPRIKDLIKKAEVDTLKEAMEQGIEEG
                     CQTFDYVLLQLYKENKITLEQALINADSANNLRLKIKLEGLKGDDAINALLDKGPHAT
                     GESPFKIQGGGAGNVRPIRKL"
BASE COUNT          739 a          981 c         1069 g          818 t
ORIGIN      
        1 ccagggttca gcgtagtagt aaacattact gaaggctggc ttggaaatag aaaggctcat
       61 ttggcgtatc ccagagtgat aggctttcgt cagccggctc atccgcctag gcactgtcct
      121 aggtggcgtg ctttacaaac tccgataaca ctagtggttc cacccgcagg ccggcgttta
      181 cgagattcac cataataaac gggggcgttc aatgtctcag gtgtcgcgtc gttttaatcg
      241 caaatccacg cgaacagacg ttatccgggt gcttctcgtc gacgatgttg agagtgtccg
      301 tgagacgctc cggagcatcc tcgccaccta tccaaacttt gaagttgtcg gggaagcctg
      361 caatggagag gaagcgttgg aatccgtgca gctgctgaag ccatcggtgg tcgtaatgga
      421 catcaacatg cctcgactag acggcattaa ggcgaccacc ctgattaaga gagtctatcc
      481 acacattgtc gtagtgggac tgtcggtcta cgccaatgac gacacgcgaa aagccatgac
      541 ggcggccggc gccaccagcg taatatcgaa ggaggcggcc gtcgaacaac tgcgtgatga
      601 aatcatcgaa tcggtcaacc ggcgttccgc agcgattcat taatcgagtt ggtcagggag
      661 acctacggtc tggttggtac cgctgaaact cagcgaggtg ttcagttttc tagttcgctc
      721 ctggggatgt tccgagcctg gtcactgatt catcgctttc tcaattgcct ggttcattcc
      781 catcacatct tcagcctgcg cgcaatattc gtacttaagg catgctgatg ctggcgccga
      841 tctgacgggc tcgccgacat taatcggcgc ttgaacccct cgccaaaata cgctcgccga
      901 cattcaccca ttacctttcg catatatgaa cttcttgcct cgggcactcc cgcaaatcga
      961 tcctctgtcc tccccatgag agacccggag tcggcggtgg tcccgtttcc attgagtttg
     1021 gctcctaagc tcggtagact tacctaccgt cgttttgcaa tggacggaga cattcatgga
     1081 gggagcatgg gaaatccggg cttgatccca cgggcgctcg ctctttttgt tgcgggcgcg
     1141 ctgacagttg ctgccgagcc gtctcccttt gccaaagagg cgcagccgcc cctcgtcgaa
     1201 acggtcaaag gaaaagacgg cgcgccgatg atcctgatcc ctgcaggtcc gtttgcgatg
     1261 ggtagcaacg agggcttacc gaacgagcgc ccagaacata tcgtcgaact cgatgcgtat
     1321 tacatcgatc agtatgaagt gaccttggcg ctataccgga agtttctcga cgcgggaaag
     1381 catgactcgc ccccgacttg ggacgacgaa gccgcgacga ctgtggggga ccggccggcc
     1441 atcggcatga aatgggcaga cgcagacgcg tactgtaaat gggccggcaa gcgcttgccg
     1501 acagaagccg agtgggaaaa agccgcacga ggcacggacg ccaggcgtta tccctgggga
     1561 tcgatgcagc ctttcgttga catcgccaat tacaaccgcg gcctctgggt gagcgaagcg
     1621 gtcacattag cggcggtgac cagcggtctc gaagggatga gtgtgcgtca tggcctgaaa
     1681 gagggaggca agagcccata cggattgaat cacatggccg gcaatgccgc cgagtgggtc
     1741 gcggattggt atgatcggga ttactatcaa aacagcccaa agaagagtcc gaccggcccg
     1801 gcacaaggtg aaaaacgagt actccgtggc ggctcttggg cggacttacc gctggccctg
     1861 cgtgtgacgg cgagaatttc ggctgagccc gagttcgagg accgaacgat cggttttcga
     1921 tgtgccctgc cggccaccgg ccagtgaccc ggtccgttac acggtcgctg gcactcacgc
     1981 gagagctctg cttcttcggg tctcacctgt ttgattgagc accatcacat gtctgatcga
     2041 aaggttaatg tcctggtgat gggtattcta ctcatcggtc tggtatgggc ctacttttct
     2101 gcgcgcaatc cagccgtgca gacggctcgg ccggtatctc tgctggcacc agagatggtt
     2161 ccacttgtga gcggcgagga gccgatcgcc acgattttta ctcgtgcggg ttgcccagta
     2221 tgccatcaga ttccaggtat tccgggagcg gagggacggg tcgggccgcc gctactactc
     2281 ggaactttcg gaccgcaacg gttacgggat cccacgtacg gaggaagggc gaagaccgtg
     2341 cacgaatatg tggtcgagtc ggtgcttgag ccgggcgtgt ttgtggtgcc gggttatcca
     2401 cctcagacga tgccgacctg gtatggcgcc aagctaagcg cgttggctct cgaaaagata
     2461 gcgacctatc ttgaacaaca aatgacggcg aaagacaccc agcagcagta gggaagagac
     2521 cgctccggcg ggatctggtt acagtttgcg aatcggcctg acgttgcccg cccctccgcc
     2581 ctggattttg aacggacttt cgcccgtcgc gtgagggccc ttgtccagaa gggcattaat
     2641 ggcgtcatct cctttgaggc cttcgagctt gatcttcagg cgcaggttgt tggcgctgtc
     2701 ggcgttgatg agggcctgtt ccaaggtgat cttgttttcc ttgtagagtt gcaacaagac
     2761 atagtcgaat gtttggcagc cctcctcgat gccttgttcc atcgcttcct tgagcgtgtc
     2821 cacctcggcc ttcttgatca aatccttgat gcgcggcgta tccatcatga tttccagcgc
     2881 agggatgcgc ttgccgtctg tggacgggac cagccgctgg gacacgattg cgcggagatt
     2941 gagggagagt tggagataga tctgcgcatg acgctcaaca ggaaaaaagt tcatgatacg
     3001 ctcgattgcc tggttggcgt tgttggagtg caaggtgccg atacagagat ggccggtttc
     3061 ggcaaacgtg atggctgcct ccatggtttc cgtatcccgg atttcaccga tgaggatcac
     3121 gtcgggcgcc tggcggagcg tgttcttgag cgcgttttgg aatgacagcg tatcgaagcc
     3181 gacttccctc tgagtgatca gtgatttttt gtgattgtgg acgaactcga tcgggtcttc
     3241 gactgtgaca atgtggccgg ggtggacgga gttccgatgg tcgatcatgg cggcgagagt
     3301 cgtcgatttt cccgaccccg tggcgccgac gacgagcacg agaccgcgct tcgtcatggc
     3361 aatgtctttc atgatctgtg gcaggcggag ctgctccacc gtctgaattt ccgctttgat
     3421 atgccggaac accagcccga cgttgccccg ttgccggaat atattcaccc ggaaccggcc
     3481 caactcctta tagtagagag ccagattcat ctccatcttc tcttcgaact cgctgcgttg
     3541 ctggccacgc atcagtgcca atgccaacgc ttccaattgt tcattggtga acggcggtgt
     3601 gttggcc
//
LOCUS       DASTQY010000211         4521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_12807, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000211 DASTQY010000000
VERSION     DASTQY010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12807"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..210
                     /gene="atpE"
                     /locus_tag="VFR82_07005"
     CDS             4..210
                     /gene="atpE"
                     /locus_tag="VFR82_07005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEU4501384.1"
                     /translation="MGMGLAAAGFAGAGVGIGYIFGKMIEAVARQPEAEGRVGKYMWI
                     GFALVEAIALYGLVIAFIIMGLRK"
     gene            353..856
                     /gene="atpF"
                     /locus_tag="VFR82_07010"
     CDS             353..856
                     /gene="atpF"
                     /locus_tag="VFR82_07010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU4501385.1"
                     /translation="MPQFESHFFSSLIFWEVVSFAILLFVLYKFAFPGILSVLEEREK
                     KIKNSLDEAEAHRLEAERRLKEYEARLAGASKEAEGILAAAKERTQRLMEENEQRLTA
                     EAERIKGEATREIDHERRKAIQEIRTQTTDLALMVAEKVVERSLTDADHRRLADEALD
                     ALSRSSH"
     gene            complement(927..1736)
                     /gene="larE"
                     /locus_tag="VFR82_07015"
     CDS             complement(927..1736)
                     /gene="larE"
                     /locus_tag="VFR82_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEU4501386.1"
                     /translation="MAMETLPDKVERLRHLVADMQSVLIAYSGGIDSTLVLKIAHDEL
                     GARAVGVTAVSPTYPAAELDTATRTAQEIGAEHAVVQTDQLAIPAFVQNDGARCFHCK
                     TDLYQLMERLRVARSMQWVLDGTNLDDLGDDRPGIKAAREWKVRSPLVEAALTKADVR
                     AAAHQLGLSSWDKPAAACLSSRIPRGVPITMEKLRRVEAAEAVLQEEGFSHFRVRDHG
                     DIARIEITPEEFERLNQPGRRSTLSTRLREIGFRFVCVDLEGYRPGGISLA"
     gene            complement(1749..3122)
                     /locus_tag="VFR82_07020"
     CDS             complement(1749..3122)
                     /locus_tag="VFR82_07020"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU4501387.1"
                     /translation="MIRTRVRPTLGRLLQLCLGTSVLALVFATWPSSPSGAAEIVPTK
                     FVTPNGLTVVVLEQHFLPIVEVHALVKSGSAQDPPEKAGLANLVASLLDEGTTSRTSK
                     QLAEQIDFVGGALEVKAAEDFTTASVRVLKKDVELGFTLLADILQHPAFLKPEFERIR
                     SQILGEMASDNDDPGHVAMKAFNQLVFHGHPYRWPLNGTEESLNKITHADVVGFYARE
                     YAPSQIILTIVGDVTAEQVTAFVQTHFGGWKKASPQPRSAKNAPPIEKKTVQLIEKDL
                     TQSNVILGHGGISRTNPDFYAVTVMNYILGAGGFSSRLMDSIRDKQGLVYGIMSHFDA
                     RLMPGSFWVNFQTRTEATNQAIQGVLAELKSIREAPVTDQELSEAKSFLIGSFPLRLD
                     STAKLAQVLAQVEFYGLGFEYFSQYPKWIERVTKEDVQRVAKQYLYPQRYALVVVGNV
                     AKAKLKQ"
     gene            complement(3119..>4521)
                     /locus_tag="VFR82_07025"
     CDS             complement(3119..>4521)
                     /locus_tag="VFR82_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU4501388.1"
                     /translation="ASFRFSPPTMIRLHFRHIPFWLLTVSTLALSVQVAAAEPHEYLL
                     SNGMKVLLVEMPKAPVATVQVWYKVGSRNEVMGRAGLSHMLEHMMFKGTAKYPKGSFS
                     RLIRKNGGMDNAFTSQDFTAYFENLAADRVQLALEMEADRMQGLILDQNELKTEREVV
                     KEERRLRTEDDPQGALVEALFAQAYLSHPYHWPVIGWFGDLDAMTLDDLQRHYDTYYS
                     PNNATLIVVGDIKAEQLLPTIKHLFEPIPRGPEPKPVATMEAEQKGERRFLLKREAQV
                     PFVMMGYRVPNYTSEDSYALDVLESILSHGKSSRLYQSLVYDQKLSLAVGAEYGLMQT
                     DPGLFYFYALVSPGQKVEVVEEALHKEIKRLQSEAPTEQELQRAKNQVEAARTFEQDS
                     NFRHAMLLGQAESVGAGWRKVDQFLERIRAVTAKDVQRVAGQYLLEDSRTVGSLIPVT
                     PKPAESPTASVQQGKP"
BASE COUNT          918 a         1265 c         1344 g          994 t
ORIGIN      
        1 cttttgggca tgggacttgc cgccgccggg tttgccggag cgggcgttgg cataggctat
       61 atctttggaa agatgatcga ggcagtggca cgacagccgg aagcggaagg ccgggtcggc
      121 aagtatatgt ggatcggatt cgcgctggtg gaagccatcg cgctatacgg gttggtcata
      181 gccttcatca tcatgggcct ccgcaaataa catgatgccg aagaaacagc ctcagacggc
      241 gttcgctgcc gggtgcaatc ctcgctgccg ggtctgagtg gttcacccgt ccgcagctgg
      301 ttggaaagct gttttcagta ttcctgaatc tttaggaaag tgagcgtgag atatgccgca
      361 gtttgaatcg catttttttt cctccctgat cttttgggaa gtggtgtcgt tcgcgattct
      421 cttgttcgtc ctctacaagt ttgcctttcc cggtatttta agcgttcttg aagagcgaga
      481 gaagaaaatc aagaacagcc tggacgaagc ggaagctcat cgcttggaag cagagcgcag
      541 gctgaaagaa tacgaagcga ggctcgctgg ggcgtcgaag gaagctgaag gcattctggc
      601 ggcggcgaag gagcggacac agcggctcat ggaagaaaat gagcagcggt tgacggccga
      661 ggcggagcgg atcaaaggcg aggccacccg cgaaatcgac catgaacgcc gaaaggcgat
      721 tcaagaaatc cgcacgcaga cgaccgacct tgccctcatg gtcgccgaaa aagtggtcga
      781 gcgcagtctt accgacgcgg atcatcgacg cttggccgac gaagcgctcg acgccctttc
      841 aaggtcgtcg cactaatcag gcagacccac agtccccggc gcttgttccc cccgagtgca
      901 gaggggtgct gtaacaatgt tcttggctag gccaagctga tcccgccggg tcgatatcct
      961 tccaaatcca cacaaacgaa gcgaaagccg atctctcgca ggcgggtact gagtgttgag
     1021 cgtcgcccgg gttgattgag tcgttcgaac tcctcggggg ttatttcgat tcgggcaatg
     1081 tcgccatgat ccctgacccg aaagtggctg aatccttcct cctgcaagac ggcttcggcg
     1141 gcctcgactc gtcggagttt ctccatggtg atggggacgc ctcgtgggat gcgcgacgac
     1201 agacaggcgg cggccggctt gtcccagctt gacagaccaa gttgatgcgc cgctgcacgg
     1261 acgtccgctt ttgtcagggc ggcttcaacg agcgggctcc gcactttcca ttcacgggcg
     1321 gccttaatgc ccggccgatc atcccccaaa tcgtcgaggt tggtcccgtc gagcacccac
     1381 tgcatggagc gggcgactcg gagtcgttcc atcagttgat acaaatcggt cttgcaatga
     1441 aagcagcgtg caccatcgtt ttgcacgaag gccggtatcg ccagctgatc cgtctggacg
     1501 acggcatgct cggcgccgat ttcttgggcg gtacgcgtgg cggtgtccaa ctcagcggct
     1561 gggtaggtcg gcgagacggc cgtgactcct actgcgcgtg ctccaagttc gtcatgcgcg
     1621 attttcagga caagggtgct gtcgatgccg ccggaatagg cgatgaggac ggactgcatg
     1681 tcggcgacaa ggtggcggag gcgttcgact ttatcgggta aggtttccat ggccatggag
     1741 cgtgtccttc actgcttcag ctttgccttt gccacgttgc cgacgaccac gagcgcatat
     1801 cgttgcgggt agaggtactg cttcgccacc cgctggacgt cttcctttgt cacccgctcg
     1861 atccatttag gatattgcga gaaatactca aaccccaggc cgtaaaattc tacctgagcc
     1921 aacacttggg cgagttttgc ggtggagtca agccgcagtg ggaagctgcc gatcaaaaag
     1981 gatttcgcct cggagagttc ctgatcggtt accggtgctt cgcggatact cttcaattca
     2041 gcaagcactc cctggatcgc ctgattcgtg gcctcagtgc gcgtttgaaa attgacccaa
     2101 aaggaaccgg gcatcagccg cgcatcgaaa tgactcatga tgccgtacac caacccttgt
     2161 ttgtcccgta tggaatccat cagccgggag gagaacccgc cggcgcctaa aatatagttc
     2221 atcaccgtga cggcgtaaaa atcgggattg gtccggctga tcccgccatg cccgagaatg
     2281 acgttcgatt gggtcaggtc tttttcaatg agctgcaccg tctttttctc gatcggcgga
     2341 gcgttcttgg cgcttcgtgg ctggggtgag gcctttttcc accctccgaa gtgcgtctgc
     2401 acgaatgccg taacctgttc cgccgttacg tcgcccacga tcgtgagaat tatctgactt
     2461 ggggcgtatt cacgggcgta gaatcccaca acgtcggcat gggtaatctt gttgagcgac
     2521 tcttcagttc cgttgagcgg ccagcgatag ggatggccat gaaagaccag ctgattgaag
     2581 gctttcatgg cgacatggcc tggatcgtcg ttgtcgctcg ccatttcgcc aagaatctgt
     2641 gagcgaatcc gttcaaactc aggcttgagg aatgccgggt gttgcaaaat atcggcaagc
     2701 agggtgaacc ccagctccac atcttttttc agcactcgga cggaggcggt cgtaaagtct
     2761 tcagcagcct tgacctccaa tgctccgccg acaaagtcga tctgctccgc aagctgcttg
     2821 gaggtgcggc tggtcgtgcc ttcatcaagc aagctggcca ccagattggc caagcccgcc
     2881 ttctcgggag ggtcctgcgc cgatccgctt tttaccaatg catgaacttc aacgataggc
     2941 aggaaatgct gttccaatac cacgacggtc aagccattcg gtgtgacgaa ctttgtggga
     3001 acgatctcgg cagcgccgga cggagagctg ggccacgtcg cgaagacgag ggctaatacc
     3061 gatgttccaa ggcacaactg cagcagacgg ccgagagtcg ggcgcacgcg tgttctgatc
     3121 acggctttcc ctgctgcact gaggcggtcg gcgattcggc cggtttgggt gtgactggaa
     3181 tcaagctgcc aactgttcgg ctgtcttcga gcaaatactg tccggcgacg cgctgcacat
     3241 ccttggctgt gaccgctcgt atccgttcga gaaactgatc gaccttgcgc cagcctgctc
     3301 cgacggactc tgcctggccc agcaacatgg catgccggaa gttggagtcc tgttcaaagg
     3361 tccgggccgc ttccacctgg ttcttggcgc gttgcagttc ctgctcagtc ggagcctcgg
     3421 actggagccg tttgatctcc ttgtgcagcg cctcctcgac aacctctacc ttttgcccag
     3481 gactgacgag cgcgtagaaa tagaagagac cgggatccgt ctgcatcaac ccgtactctg
     3541 ccccgacggc cagcgaaagc ttctggtcgt acaccaagct ctgatacagc cgcgaactct
     3601 ttccgtgcga gagaatcgat tccaggacgt ccaacgcata ggagtcctcg ctggtgtaat
     3661 tggggacgcg ataccccatc atcacaaacg gcacctgtgc ttcacgcttg aggagaaagc
     3721 ggcgctcccc tttctgctcg gcctccatgg tcgcaaccgg tttgggctca gggccacggg
     3781 gtataggctc gaacagatgt ttgattgtcg gcagcaattg ctccgctttg atgtcgccga
     3841 cgacaatcag cgtggcattg ttaggcgagt agtacgtgtc atagtgccgc tgcagatcgt
     3901 cgagcgtcat ggcgtccaag tcgccgaacc agccgatcac gggccagtgg tagggatggc
     3961 tgagataggc ctgcgcaaac agggcctcga ccagcgcgcc ctgcggatcg tcttcggtcc
     4021 taaggcggcg ctcttccttg acgacttcgc gctcggtttt gagctcattc tgatccagta
     4081 tgaggccttg catcctgtcg gcctccattt ccaaggcgag ctgcacgcga tcggcagcga
     4141 gattctcaaa ataagccgta aaatcttgac tggtgaatgc gttgtccatc cccccgtttt
     4201 tgcggatcag acgcgagaac gaacccttcg gatacttcgc ggtgcctttg aacatcatgt
     4261 gttcgagcat gtgcgagagg ccggctcgcc ccatcacttc attgcgcgat ccgaccttgt
     4321 accaaacctg aaccgtcgcg acgggagctt tgggcatctc gacgagcagc actttcatgc
     4381 cgttcgagag caggtactca tgcggttctg cggcggcgac ttgaacggaa agtgcgaggg
     4441 tgctgacggt gagcaaccaa aaggggatgt gccggaagtg cagcctgatc atggtcggtg
     4501 gtgagaaacg gaacgatgca t
//
LOCUS       DASTQY010000212         6397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1600463, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000212 DASTQY010000000
VERSION     DASTQY010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600463"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="VFR82_07030"
     CDS             complement(<1..1126)
                     /locus_tag="VFR82_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501389.1"
                     /translation="MNESLAILAGPVARSWWRETGAPPTIGQFRNQYSSSISWLAGIF
                     LVAAFTAVLLMASTTSKIDRIDAPEQALSLMVGRTMDAQEGLSRAPAWEQRMMRWVSG
                     DGEAERAQAIAWYQELVARSDDPAVSLQQAILQAESGQLSQALLSAHEWRTREEPLPA
                     YGDIIRVAYGAERSPDRQTVEALQSRMADVLPVGWFYDRLMMRLAQRAGHAELAAVIN
                     EEFASRGDRLYHYSRRLTVMELVVMLAGTLLLFLIWQKHRAGMNVVRLHDPGLPPPWP
                     GSVGTAVLLRGGAIGAIIMAVCLLYLPPENASLRALAIPLTNVPVLALAYYHLFRPAG
                     MTFEDGFGLSLPWSHIGRLMTAVLAVVAAGLWGEWLMDRVA"
     gene            1275..2468
                     /locus_tag="VFR82_07035"
     CDS             1275..2468
                     /locus_tag="VFR82_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEU4501390.1"
                     /translation="MTIPEFTPPRRLLLGPGPSLVHPRVLRALSSPLLGHLDPVFLSV
                     MNDIQALLREVFQTANRFTIAISGTGSAGMEASIVNVVEPGDCVVVGINGVFGSRMAS
                     VVERCGGKVIRIEAAWGTCIEPDAIEQALVRSGPVKAVAIVHAETSTGAWQPLEDIGS
                     LCRRHEALFIVDAVTSLGGIPVEVDRLGIDVCYSGSQKCLSCPPGLSPFTMNDRALGI
                     MKSRRSQCPSWYLDMGLIDGYWSEGMRAYHHTPPVSMLYALREALRLVDEEGLPARYK
                     RHQMNSDALVAGLKELGLVPLPPAGRALPMLNCMMLPAHIPEADIRAKLLSYYGIDIG
                     AGLGPLKGKVWRIGLMGESSTEAHVLTLLNALESLFIEGGWLGTPGVALQAASRIYSR
                     LPDRR"
     gene            2476..3072
                     /locus_tag="VFR82_07040"
     CDS             2476..3072
                     /locus_tag="VFR82_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEU4501391.1"
                     /translation="MDNKPLLWVLGGGAFAFTAVVAYWIFAITLANQLKSDQVSPQKT
                     AEFVHAILEANRNNYTDNVVVKLSRDGIEAHEHWRDERGVPLPAQFLLESGRLVAVKD
                     LGFSFRLASLTPIYAWNGANSDLERVGLAAVQQDPSQPHYGIVRRGELRLFQAVYADR
                     AVSQACVDCHNRHPNSPRRDFKLNDVMGGIIITFPIGS"
     gene            3077..4288
                     /locus_tag="VFR82_07045"
     CDS             3077..4288
                     /locus_tag="VFR82_07045"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU4501392.1"
                     /translation="MTIAIHNVRLLDGSGHAIERATVIVQDTRIHAAGPSRTVNIPRG
                     ASRIDGRGLTLLPGLIDCHVHFSLGAEPDVVKAIEQETPSETLLKAAQSARQTLEGGV
                     TTVRDVGSRDHSIFTLKRAIDKGLTEGPRIIGAGLAICMIGGHARFIGQEVEGVEQVR
                     KVVREQIEAGAQVIKVIASGGVLTPGTSPDQAQMTMEELRTAVDEATLAGRKVAAHAH
                     AAAGMKNAIRAGVHSIEHATLMDQEAAALMLDHGVYMVPTLSALATTAACRRGCGIPD
                     SALEKAKTMTKRHETSFRQAHRRGLLIAMGTDAGTPFNRHGDNAQELERMVAFGMSPM
                     EAIIASTSAAARLIGLDDQVGMIARGKEADLLLIDGNPLRKVELLRDRSRITGVMKAG
                     RFVAGPLRQPA"
     gene            complement(4310..4948)
                     /locus_tag="VFR82_07050"
     CDS             complement(4310..4948)
                     /locus_tag="VFR82_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU4501393.1"
                     /translation="MPRVDFYRILGVSREASEDVIKKAYRKLVFEHHPDRNPDSKQAE
                     DKIREINAAYEVIGDAEARRNYDRLNWGDEFAPDVVDVAVLLEEMERKLFDEGRKELF
                     ALLVKDVTKIKAELALIRDRVVKEQGYDSFKETLVHHRATEVMPELVTDEMSGRKTRL
                     VEVATQMMVSQGVVARNDEGGIRSLRHRLDEQFNRGRLHGFASALELFYERR"
     gene            5134..6372
                     /locus_tag="VFR82_07055"
     CDS             5134..6372
                     /locus_tag="VFR82_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4501394.1"
                     /translation="MHRDQWLVLFVAWLGWVFDAMDATIYAIVLHPALHELLHTSSGP
                     PTADSIGWYGGIIFSIFLIGWAIGGITFGILADRFGRTKVLIATILIYAVFTGAAALS
                     QEWWHLALYRFLTALGIGGEWAAGAALVAESWPEDKRAKAAGILQSAWAVGFFLAALC
                     NLALKDAYGWRGLFLIGILPAFVALLIRWWVKEPPRWAHAHAQRIVPLSAIFEGDLKR
                     ATLVGSALAFVAVFGLWGSTNWAPTLVRELPEFQGADSATLTRSVSYAIMALNAGAIF
                     GYLGFGPLADRFGRRAVFAFMCVGSFIMLPTTYLLPSHYSGVLLLLPVLGFFNNGIFS
                     GFPIYLPELYPTRLRATGAGFCFNAGRILASVSPFMTGWLVTTLGTFGRAASTVALIY
                     LAGLVVLWFAPETKGKPLTD"
BASE COUNT         1261 a         1933 c         1859 g         1344 t
ORIGIN      
        1 ccgccacgcg atccatcaac cactcacccc agaggccggc agcgacgacg gcgagcaccg
       61 ccgtcatgag tcggccaatg tgtgaccagg gcaggctgag accgaatcca tcctcgaacg
      121 tcatacccgc cggccggaag aggtggtaat aggccagcgc gaggaccggt acattcgtta
      181 atgggatggc tagggctcgc agagacgcat tctcaggagg cagatagagg aggcacaccg
      241 ccatgattat cgcaccaatc gcaccgccgc gcagcaagac agccgtgcca acactgcccg
      301 gccacggcgg tgggaggccg ggatcgtgca atcggacgac gttcattcct gcgcggtgct
      361 tctgccagat caggaagagt aaaagggttc ccgcgagcat gaccaccaac tccatcacgg
      421 tcaatctgcg ggagtagtga tacaggcgat cgccgcgcga ggcgaactct tcgtttatga
      481 cggctgccaa ctccgcatgc cccgctcgtt gggctaatct catcatgaga cggtcgtaaa
      541 accatccgac cggcagcaca tccgccatgc ggctttgcag cgcctcgacg gtttgacgat
      601 ctggagatct ctccgcgcca taggcgaccc gaatgatgtc tccataagca ggaagcggtt
      661 cttcgcgagt gcgccactca tgtgctgaca agagcgcctg ggagagttgt ccgctttccg
      721 cttgcagaat ggcctgctgc aaggaaacgg ccggatcatc ggagcgagca accagctcct
      781 ggtaccatgc gatggcctgt gcccgctcgg cttctccatc gcccgacacc cagcgcatca
      841 ttcgttgctc ccaggcagga gcccttgaga gcccctcctg agcatccatc gtgcgcccga
      901 ccatcaagct aagtgcctgt tccggtgcgt cgatccgatc gattttcgag gtggtcgaag
      961 ccatgaggag gactgccgta aacgcagcga cgagaaaaat cccagccagc cagctgatcg
     1021 aggaggaata ctggttgcgg aattgtccga tagtcggtgg cgcccccgtc tcccgccacc
     1081 aggaacgagc gaccgggcca gccaagattg caagtgattc gttcatctcg cctggactat
     1141 aacatggcta tttttgaccc agaaacccct caaaagtaac gcgctgtcct cctcgcagaa
     1201 cgcgaactgg ctgaacgaag cgagagtccg ctatactgcc gagccaagat gctcttccca
     1261 tcaagcagcc agatatgact attccggaat ttacccctcc gcgacgcctt cttctcggcc
     1321 cgggcccgag cctcgtgcat ccccgcgtct tgcgcgccct ttcatccccg ctgttggggc
     1381 acttggatcc agtttttctc agcgtcatga acgacattca ggccctgttg cgcgaagtgt
     1441 ttcaaaccgc caaccgcttc acaatcgcta tttcaggaac cggctctgcc ggcatggaag
     1501 cgtcgatcgt gaacgtcgtc gagcccggtg attgcgttgt cgttggcatc aacggcgtct
     1561 ttggaagcag gatggcctcc gtggttgaac gatgcggtgg aaaagtgatt cgaattgaag
     1621 ctgcgtgggg gacctgcatc gagccggacg caatcgaaca ggcgctggtc cgttcgggac
     1681 ctgtcaaggc ggtcgcgatt gtgcatgcgg aaacgtcaac aggagcctgg cagccactcg
     1741 aggacatcgg gtccctgtgt cggcgtcacg aggcgctgtt cattgtcgat gccgtcacgt
     1801 ctcttggcgg cattcccgtc gaggtggacc ggctcggcat cgatgtatgc tacagcgggt
     1861 cgcaaaaatg cttgagctgt ccgcccggat tgtcgccgtt taccatgaat gaccgggctt
     1921 tgggcatcat gaaatcgcga cggagtcagt gccctagttg gtatctggac atgggattga
     1981 tcgacggcta ctggtcggag ggtatgaggg cctatcacca caccccgcct gtctcgatgc
     2041 tctatgcgct gcgtgaagcg ttgcgactcg tcgacgaaga gggcttacca gcccgctaca
     2101 agcgtcatca gatgaacagc gatgcgctgg tggccggact taaggaactc ggattggtgc
     2161 cgctcccacc tgcgggtcga gcgttgccca tgttgaactg catgatgctc cctgcccata
     2221 tccctgaagc ggatatccgc gcgaagttgc tctcctatta tggaatcgac atcggagccg
     2281 gtcttggtcc gttgaagggc aaggtgtggc gtatcggtct catgggggaa tcttcaacgg
     2341 aggcccatgt cctcacgctt ttgaacgccc tcgaatcgct cttcattgaa ggaggatggc
     2401 ttggcacgcc cggtgtcgcg ctgcaagccg cctcgcgaat ctatagccgt cttcctgacc
     2461 ggaggtaacg ttcgtatgga caacaaaccc ttgctttggg tgcttggcgg cggggccttt
     2521 gccttcaccg ctgtcgtcgc ttactggatc ttcgcgatca cgttggcgaa ccagctgaag
     2581 tcggaccagg tctcaccgca aaagaccgct gagttcgtcc atgcgatcct ggaagccaat
     2641 cgcaacaact acacggacaa cgtcgtggtc aaactcagcc gcgacggcat cgaggcgcac
     2701 gagcattgga gagacgaacg tggagtcccg cttccggctc agttcctctt agaatctgga
     2761 cggctggtcg ccgtcaaaga ccttggcttt tccttccgtc tggccagctt gacgccgatc
     2821 tacgcctgga acggtgcgaa tagcgatctc gaacgcgtcg gattggcggc cgttcaacag
     2881 gatccttcgc agcctcacta tggtatcgtg cgacgcgggg agctgcgttt gtttcaagcg
     2941 gtctatgccg atcgcgccgt ctcccaagct tgcgtcgatt gccacaaccg acatcccaac
     3001 agcccccgcc gggacttcaa gctcaacgac gtcatgggcg gaatcatcat cacatttccg
     3061 atcggaagct gatcccgtga ccatagcgat ccacaacgtt cggctgctcg acgggagcgg
     3121 tcatgcgatc gaacgggcca cagtcatcgt gcaggacacg cggattcatg ccgctggtcc
     3181 cagtcgcacc gtgaacattc cacgcggcgc ctcccgcatc gatggccgcg gactcaccct
     3241 gcttccaggt ttaatcgact gtcatgttca tttttctctg ggggccgaac ctgacgtggt
     3301 caaggcgatc gaacaggaaa caccgtctga aacattgctt aaggccgctc agtcggcgag
     3361 gcaaacgctt gaaggcggcg tgaccacggt tcgcgatgtc ggctcgcgtg atcactcgat
     3421 ttttacgctc aaacgggcga tcgacaaagg cttgactgag ggacctcgca ttataggcgc
     3481 gggactcgcg atctgcatga tcggtggcca tgcacgattt atcggccagg aagtggaagg
     3541 cgtcgaacaa gtccggaaag tcgtgcgcga gcaaatcgag gccggtgccc aggtcatcaa
     3601 ggtgatcgcc tcaggcggcg tcctcactcc cggcacgtcc cccgatcaag cgcaaatgac
     3661 gatggaagaa ctgcggaccg ccgtggacga agcgaccctc gcgggacgaa aagtcgcggc
     3721 gcatgcgcat gcagcagcgg ggatgaagaa tgcgatccgc gccggcgtgc attcgatcga
     3781 gcatgccacc ttgatggacc aggaagccgc cgcgctcatg ctggaccatg gcgtctatat
     3841 ggttccgaca ctttcagcgc tcgccacgac ggccgcctgc cgccgaggtt gcggcatccc
     3901 tgatagtgcc ctggagaagg ccaagaccat gacgaagcgt cacgaaacca gcttccggca
     3961 ggcgcacagg cgtggactgc tgatcgccat gggcaccgat gccggaacac ccttcaacag
     4021 gcacggagat aatgcccagg agctggagcg catggtcgca ttcggtatga gcccgatgga
     4081 agcgatcatc gcctcaacat cggccgccgc gcgattgatc ggcttggacg atcaggtcgg
     4141 aatgatcgca cgtgggaagg aggccgatct cctcctcatc gacggaaatc cgctgcggaa
     4201 agtggaactg ctgcgcgacc ggagtcgcat taccggcgtg atgaaggccg gacgcttcgt
     4261 ggccggcccg ctccgccagc ccgcgtgaga cgcatggaat tgcatcgcct catcgccgtt
     4321 catagaacag ttccaacgcc gacgcaaagc cgtgcaggcg ccctctattg aattgctcgt
     4381 cgagtcgatg gcgtaacgaa cgaatgccgc cttcgtcgtt gcgagcgacc acgccttgag
     4441 acaccatcat ctgggtagcc acttccacca ggcgtgtttt gcggccgctc atttcatcgg
     4501 tcaccagttc cggcatgacc tccgtcgcgc gatggtggac gagcgtctcc ttgaatgagt
     4561 cgtatccttg ctctttgacc acacgatccc ggatgagcgc caattccgcc ttgatcttcg
     4621 tcacgtcctt caccagcagc gcaaagagtt ccttccgtcc ttcatcgaac agtttccttt
     4681 ccatctcctc aaggagaacg gcgacatcaa ccacatccgg cgcgaattca tcgccccagt
     4741 tcagccggtc gtagttgcgc cgcgcttccg catcaccgat gacctcgtaa gcggcattga
     4801 tctcgcggat cttgtcttcc gcctgcttgc tgtcaggatt acgatcagga tgatgctcga
     4861 aaaccagttt ccggtaagcc ttcttgatga cgtcttccga cgcttcccgg gacacgccca
     4921 aaatccgata gaaatccacg cgcggcatgg gcgcgactat aacaggatgt cgaaaagagc
     4981 ttccagcttc accttgactc cgccagggaa gatccttagc cttgcgcagc ccatgtcccc
     5041 tttgtggact catccgagga cactcagccc ccaacgatcg ttgcagttca ccccgaaagg
     5101 caatcggtga gcaggcaatc ctggtggcgg ggtgtgcatc gagatcaatg gctcgtgttg
     5161 tttgtcgctt ggctcggctg ggtcttcgat gccatggatg ccacgatcta tgcaatcgtt
     5221 ctgcatccgg ccttgcacga gttattgcac acctcgagcg gcccaccgac cgccgacagc
     5281 atcggctggt acggcgggat tatcttttcc attttcttga tcggctgggc aatcggcggg
     5341 attacctttg gaattctggc ggaccgcttt ggacgaacca aggttctcat agcgacgatc
     5401 ctgatctatg ctgtgttcac cggcgcagcg gccctatcac aggaatggtg gcatctcgcg
     5461 ctctaccgct ttcttacggc actgggaatc ggcggagagt gggccgcggg ggcggccctt
     5521 gtcgctgaat cgtggccgga agacaagcgg gccaaggcgg caggtatttt acagtccgcc
     5581 tgggcagtgg ggttttttct ggcggccctc tgcaatcttg cactgaaaga cgcgtacgga
     5641 tggcggggtc ttttcctcat cggcatcttg ccggccttcg tcgccttact gatcaggtgg
     5701 tgggtgaagg agccaccacg ctgggcgcac gcccacgcac agcgaatcgt cccattgtcg
     5761 gccattttcg aaggagattt gaaacgagca acactggtgg gttctgccct ggctttcgtg
     5821 gcggtgttcg gtctttgggg atcgacgaac tgggcaccga cgctcgtgcg cgagttaccg
     5881 gaatttcagg gagcagactc cgcaacactc acacgatcgg tcagttatgc cattatggcg
     5941 ctgaatgcgg gagcgatctt cgggtatctc ggctttggcc cgttagctga ccgattcgga
     6001 cgccgagcgg tctttgcgtt catgtgtgtc gggagcttta tcatgttgcc tacgacgtat
     6061 cttctgccgt ctcattacag cggcgtcttg ctgcttttgc cggtcctcgg tttcttcaac
     6121 aacggcattt ttagcggatt ccccatctac ctgccggagc tttatccgac gaggctccgc
     6181 gccaccggcg ccggcttttg cttcaacgcg ggccggatcc ttgcctcagt ctcccccttc
     6241 atgaccggct ggctggtcac gacgcttggt acattcggcc gagcagccag tactgtggca
     6301 ttgatatatc tggccggctt ggtcgtcctg tggttcgcgc cagaaaccaa aggcaaaccg
     6361 ctgacagact aagactaatt gtgctgtacc cttgact
//
LOCUS       DASTQY010000213         3334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1253857, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000213 DASTQY010000000
VERSION     DASTQY010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253857"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFR82_07060"
     CDS             complement(<1..192)
                     /locus_tag="VFR82_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501395.1"
                     /translation="MRSVLKALVPDGMKPALRRVEQDLTGWKDHWQLDRIRGLSFAAK
                     PGTLLPCGPYKVRINDGPNF"
     gene            complement(194..1372)
                     /locus_tag="VFR82_07065"
     CDS             complement(194..1372)
                     /locus_tag="VFR82_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4501396.1"
                     /translation="MKILIANTDYFEFLSGLYQGVPGLSQKGYDEQLQVRYDSRFGVA
                     DFYSRHMRALGHEAHDIYVNNLAMQRAWMREHSTEARDAQARTSPGNLLESLLEYADR
                     SVLGRVSSRIRSLLQRRDDEGCRILAAQVAYHRPDVFLNQAMDGIDSAWMHRIKPWVR
                     LLVGQHAATTLPDDDDYRCYDLVVSSFPPTVEFFARKGIPAVLHRLGFDPDVLATHPE
                     PVRVYPVTFVGSLSDVHTTRIEWLEHLCVRIPELRIWGPGIERLKASSPLRQRHMGLV
                     WGNQMYDVLRRSQITLNHHGDVAPYANNCRLFEATGMGAMLLTDWKANLQDMFQIGKE
                     VAAYRTTEECAEMIRYYLKHDEARMAVARAGQTRTLRDHTYRSRMQEFVELTQKRMVA
                     "
     gene            complement(1369..2286)
                     /locus_tag="VFR82_07070"
     CDS             complement(1369..2286)
                     /locus_tag="VFR82_07070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4501397.1"
                     /translation="MVHVLIPAHNNKPEVIAVLACLERQRSVELEVLLVDDGSTDGTR
                     EEVRRLFPLVRILSGDGSLWWTGANVIGVAHILGTAQDRDFILLLNNDVSMEDDYVSR
                     LVECSESLGRALVGSTIVDDQRRDRLMAGIRLDRRLRINQNSDRAAIEQSPHDVHVDV
                     LPGRGTLVPVEVFRRIGTFNRRRLPHYGADYEFSIRAKRAGFRLAVSHDARVFAKLGI
                     TGFYPSDRPKISVRECVRLLCSKKSTANLRYYLNYVWMCSESGYRLRNTISHGVGLLL
                     DTLGKTAVGVPLQICMRLWLRADRTVKAS"
     gene            complement(2286..>3334)
                     /locus_tag="VFR82_07075"
     CDS             complement(2286..>3334)
                     /locus_tag="VFR82_07075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEU4501398.1"
                     /translation="KPVGVRPVRKMIKNPAISVVVCTHNRAALLEQALESLVAQTLDR
                     SQFEIIIVNNCSTDDTAGVVERYKAHRAGDELLRSVDEPVLGLGHARNTGWRAAQADY
                     VAFMDDDAKADARWLELALDLIRQHPTTPVAIGGQIRPLYVAPKPAWYKDEYEVRSWG
                     QRSRHLVTGESFSGSNMIVSKAILRLLNGFDASVGMRGERVSMGEETVLFGKIWHALG
                     DQALLVYSPDLVVYHAVGKHNMHLRYHLRRWFVAGQVACRLDAPSGVHNRIARLRANV
                     TTIRMLIRSAVTERKHFSDYRPWIVERLGPVALELGRLLACLGLHIPVRRANLSAELK
                     AAGKSGRGHSIEAV"
BASE COUNT          726 a         1007 c          905 g          696 t
ORIGIN      
        1 aaaattggga ccgtcgttga tacgcacctt gtagggcccg cagggcagca gcgtcccggg
       61 cttggcagcg aacgagaggc ctcgaatccg atccagctgc caatggtcct tccagccggt
      121 gagatcctgt tccacgcgtc gcagggccgg cttcataccg tcgggaacga gcgctttcaa
      181 gacggatcgc atatcaggcc accattcgct tttgagtgag ctccacgaac tcctgcattc
      241 ggcttctata ggtatggtcg cgtagtgttc gcgtctggcc cgcccgggcg acggccatac
      301 gagcctcgtc gtgcttgaga tagtaacgaa tcatctccgc acactcctcg gtcgtacgat
      361 atgccgcgac ttcctttccg atctgaaaca tgtcctgcag attggctttc caatcggtga
      421 gcagcatcgc tcccatgccg gtcgcctcga acagacggca gttgttggca tagggggcca
      481 cgtcgccgtg atgattcagc gtgatctgtg atcgtcgaag cacgtcgtac atctggttgc
      541 cccacacgag tcccatatgg cgctgtcgaa gcggagagga cgccttcagc cgctcgatac
      601 caggccccca gatgcgaagc tcgggtatcc tgacacagag atgttccagc cattcaatcc
      661 gggtcgtgtg gacgtccgac agactgccga caaaggttac cggatacacg cgaaccggct
      721 cggggtgcgt tgccagcaca tcgggatcga agcccaatcg atggaggacg gcgggaatac
      781 ccttcctcgc aaaaaattcc accgtcggcg gaaacgaaga gacgacgaga tcatagcagc
      841 ggtagtcgtc gtcatccggt aaagtcgtcg ccgcatgctg cccgaccagt aaccgaaccc
      901 acggtttgat cctatgcatc caggcgctgt cgatgccgtc catcgcctgg ttcaggaaca
      961 cgtccggccg atgataggcc acctgcgcgg caaggattcg gcaaccttcg tcgtcccgac
     1021 gctgaaggag ggagcggata cgagagctca cgcgcccaag gactgaccga tcggcatatt
     1081 ccagtagcga ctccaacagg tttcccggcg acgttcgcgc ctgtgcgtct ctggcttccg
     1141 tgctgtgctc cctcatccat gcccgttgca tagccagatt gttgacatag atatcgtgcg
     1201 cctcgtgtcc taacgctcgc atatgtcttg agtaaaagtc ggctacccca aaccggctgt
     1261 cataccgaac ctggagctgc tcgtcgtagc ccttttggct caacccgggg accccctgat
     1321 agaggccact caggaattcg aagtaatccg tattcgcgat caagattttc acgaggcttt
     1381 caccgtccgg tccgccctca accagagccg catacaaatc tgcagtggca cacccactgc
     1441 tgttttaccg agcgtatcca acagcaatcc aacgccgtgg gaaattgtgt tgcgcagtct
     1501 gtaaccggac tctgaacaca tccacacata attcaggtag tagcgaagat tggcagtgga
     1561 tttcttcgaa cacagcaatc gcacgcattc tctgaccgaa attttcggtc ggtccgacgg
     1621 atagaagccg gtgatcccta atttggcaaa cactcttgcg tcgtgagaaa cggccaacct
     1681 gaatcctgcg cgcttggctc tgatgctgaa ttcataatct gcgccatagt gaggaagccg
     1741 ccgccggttg aacgtgccga tgcgacgaaa cacctcgacc ggcaccaacg ttccccgtcc
     1801 aggcagaacg tcgacatgga cgtcatgtgg agattgttca atggctgctc tgtcggagtt
     1861 ttgattgatg cgaagccgtc gatcgagtcg gatgccggcc atgagacgat cgcgtcgttg
     1921 gtcgtccacg atcgtggacc cgacgagcgc tcggccaagg gattcgctgc attccaccag
     1981 gcggctgaca tagtcgtctt ccatagagac gtcattgttg agaagtagaa tgaagtcccg
     2041 atcctgcgct gttccaagaa tatgggcgac tccgatgacg ttggcgcctg tccaccacaa
     2101 gctgccgtcg cctgagagaa tgcgaaccaa gggaaagagt cgacgtacct cctcccgggt
     2161 gccgtcggtc gaaccatcat ccaccaacag cacctcgagc tcgacggatc gttgtcgctc
     2221 aaggcaagcg agaactgcta tgacttccgg cttgttattg tgcgcaggaa tcaagacgtg
     2281 aaccatcaca ccgcctcaat cgaatgacct ctgccactct tccccgccgc tttcagttcc
     2341 gccgacaggt tcgctcttcg cacggggatg tgcagcccta ggcaggccag taatcgccct
     2401 aactccaacg cgaccggtcc taagcgttcg acgatccatg ggcgataatc ggaaaagtgc
     2461 ttccgctcgg tgaccgctga ccgaatcagc atgcgtatgg tggtgacgtt tgcacgaagg
     2521 cgagcaatcc gattgtgcac gccggagggc gcatccagcc ggcaagcaac ctgccccgcg
     2581 acgaaccaac ggcggagatg atagcgcaga tgcatgttgt gtttgccgac tgcatggtag
     2641 acaacgagat ccggtgaata caccaacagg gcctgatcgc ccagggcatg ccagattttt
     2701 ccaaaaagaa cggtttcctc tcccatcgac acccgttctc cgcgcatacc gaccgacgcg
     2761 tcgaaaccat tcaacaggcg aagaatcgcc ttcgatacaa tcatgtttga acctgagaac
     2821 gactcacctg tcaccaaatg tctgctccgc tgcccccaag accgtacttc gtattcatcc
     2881 ttgtaccagg caggcttcgg cgcaacgtac aacggccgta tttgaccgcc tatggcaacg
     2941 ggggttgttg gatgttgtcg aatgagatcc agtgcaagct caagccagcg ggcatcggcc
     3001 ttggcgtcat cgtccatgaa ggcaacatag tctgcctgag cagcacgcca gcccgtgttt
     3061 cttgcgtgac ccaatcccaa caccggttcg tccaccgatc gcaacagttc gtccccggct
     3121 cggtgagcct tgtagcgttc aaccacgccg gcggtgtcat cagttgagca gttgttcaca
     3181 atgatgattt caaactgact gcggtccagg gtctgggcaa ccagactctc taaggcctgt
     3241 tccaacagcg ccgcccggtt gtgggtgcac acgacgacag agattgcagg gttcttgatc
     3301 atcttcctta cagggcgcac gccaactggt ttga
//
LOCUS       DASTQY010000214        12679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_657590, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000214 DASTQY010000000
VERSION     DASTQY010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 12679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12679
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657590"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(529..1005)
                     /locus_tag="VFR82_07080"
     CDS             complement(529..1005)
                     /locus_tag="VFR82_07080"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEU4501399.1"
                     /translation="MRRQTRTPISILRSARVGALILIALAGMIGSAQASQPGLHVEIL
                     GIRNSIGAVACALFQGPEGFPTEFLRFATNIMMVQVRATKATCDFADIAPGTYALAVI
                     HDENRDGELATNWMGKPSEGYGFSNDAKGTLGAPSFEAASFSYNGERLNMTIALQY"
     gene            1410..2378
                     /locus_tag="VFR82_07085"
     CDS             1410..2378
                     /locus_tag="VFR82_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501400.1"
                     /translation="MQSTYEQWSQGRSHIQWGAIVAGLAVGLAVQMLFTLLGLAIGAW
                     SIDLREAQPAGGIPLATGLWTGMSMLMSAFVGGYAASRLSGASNRTEGLYHGAVLWGV
                     TSVVFAWLGTTALSFMIGGLFTTVGGGLQMLSQGVGTAVSAASAKIPGTGITNLTAAD
                     LRNQIESVLKATEKPELQPSTVSKDIDKKSTQAREGQSVHKLSDSTIGEIRDKLVAMD
                     RDAAVNVMVNKLGMSEKQAQEVAQTAIGMVASVKETGKEIKEKSMDIGNATMTGVGSA
                     AWWLFVLALLSLGASLTGGALGTGDEILSRLEGKAYPLEVKPAMSN"
     gene            2532..2879
                     /locus_tag="VFR82_07090"
     CDS             2532..2879
                     /locus_tag="VFR82_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501401.1"
                     /translation="MTPLFTKQTIWSLTILLSGFLAAAPIQAQDREPATGAPGSNAIT
                     ESDIAAGSVEDTIEACKARIPKDASIGQVMIAEQSCWRDENERNPAQAIPGARSTSLK
                     GGGSVAMGTEGRH"
     gene            3158..5083
                     /locus_tag="VFR82_07095"
     CDS             3158..5083
                     /locus_tag="VFR82_07095"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU4501402.1"
                     /translation="MDLIRPVRPSRSTESPPRRLLLVDPYPLNNPYHLTASERRAVWF
                     PKLSLPVIAAYTPESWSVDLVDEAVQEVDFDNPCDLVGISVMTCYAPRAYEIATEFRK
                     RGKTVVLGGVHPTYCPEEALQFCDAVVCGEAEDLWPEVIADFMAGSLQRKYQMTAFPA
                     LENYKSPRVELLSPDAYMTRQCSFTTRGCHFDCEFCSVSPFNGKTTRRRPVEEVIKEL
                     TQVKRWVCGELVDRMSNDTLWNAFLTGLRIRLGIEDGSIVAFVDDLHNSNRAYCRELW
                     AALKPLNIKWGCQSTLFLGDDEDMVKLAAESGCVSVFVGMESLDEGALEETNKPFNRV
                     DKFAQEIKMFHDHGIMVNPGIVFGFDHDDESVFERAVEFLTKNQVELAYFNVLTPLPG
                     TALFDRYTAAGRIFDHDWGHYDGKHVVYRPSRMTPERLQEGFYWANHQFYSLPSIWKR
                     LSGTSQRFVPRLLMNREFRKLVKRSCPPGALSVVTTALKILQAKLPTLQAGHLIPNAL
                     HVNVMKEENKESEVSRTGSRWLSIKAKQHDQFAALFVDLEGTLDQATTKELVKRITEA
                     AKRAKLDIIINFEHLKDATPEALRALGNSELWRGVVPYAKIHYRKFKSAFEASLADLS
                     INLLCEDDSQSKAKACD"
     gene            complement(5080..5295)
                     /locus_tag="VFR82_07100"
     CDS             complement(5080..5295)
                     /locus_tag="VFR82_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501403.1"
                     /translation="MKPDNIIIDHILNVIALAPGARIEEVAKRTPELTLREVFYTLCY
                     LSQKGRLRLIVDGQGGFAVTTIVRSFN"
     gene            complement(6001..6921)
                     /locus_tag="VFR82_07105"
     CDS             complement(6001..6921)
                     /locus_tag="VFR82_07105"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4501404.1"
                     /translation="MSEETSGVDHYERGLLLKKVQIFDSALQEFQQATRDQQQAGKAF
                     AQAALCLKSLKRHDEAVTAFRQALETGAFSTRERVHILYLLGQTLESLNRDFEALVVY
                     RRIRREEPHFQDVDARIEELSSNPFDPGAGPQSPVDQDGDVANLWRQLKPQLTSLMNQ
                     TWQKLAGYRETIELARWGTRTAVNRGNSPRSINRACPGVNVRKEKRRHSRVAVHLSSQ
                     FSSKSQIVGGEGELRDLSPSGCRISSPVGVPLGTTLECWIYPQEGNPFTVEEATVQWS
                     GYREFGLAFTKLRPSVQRQIADMCRKIAPI"
     gene            7528..7947
                     /locus_tag="VFR82_07110"
     CDS             7528..7947
                     /locus_tag="VFR82_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4501405.1"
                     /translation="MSDATTILLIDGNTQEREYYAHYLHAASATYEVAQAAMGGSGLD
                     LCARQPIDCVVLEIDLPDMSGFEVLARLVPRTYHPEIAVIVLTRLSNPFLLELAIKNG
                     ALGAFHKKTVSGDILDKTIRNAITTVQTLNSVSMLAR"
     gene            8154..8735
                     /locus_tag="VFR82_07115"
     CDS             8154..8735
                     /locus_tag="VFR82_07115"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU4501406.1"
                     /translation="MHENNHSTPCSDAQLPIDEFICHASEHVEQMLKAGVPRCEILTR
                     LARAGEVLAGPDTSVSILVIDEDGLLRNGSSPNLPLDYLNAIDRLKPDAKVGTCAAAA
                     ATGSVVITPDFFADDKWAELRHLPSSLGYVGAWSMPILAPGGPVLGTFGTYFRARRTP
                     TTVEKKGVELLAAAAALVLTKVESEQSGDSYRP"
     gene            10024..10434
                     /locus_tag="VFR82_07120"
     CDS             10024..10434
                     /locus_tag="VFR82_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4501407.1"
                     /translation="MINVLLVDDYPIMQQLLRDILQRYPDICVVGEAQDGEDAVLQSM
                     KLKPTVVIIDINLPTISGMEATRLIKRQRPSTTVIGLTAGAPEQIVTAMQDAGAAAVL
                     RKEDLLSVLYPTMIEAIRSSAKPAFASASASPKH"
     gene            10902..12443
                     /locus_tag="VFR82_07125"
     CDS             10902..12443
                     /locus_tag="VFR82_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4501408.1"
                     /translation="MRLMPFASSGKETFLGKRPQGRKYLVGSLVLSALAIRMLLAQRK
                     QAEGELRAIRWLLREGGKPFQMPKPQPYGDLTELNTELTVLRRVGKELLINVAGDALH
                     LIGTSGAVCEKNGDYASCLVASDWCRFLDQASREQCGTVDNRAALTSGRWHCRESGRE
                     ASILAMETGKPVDIPCKGGINLYAAPIMVDGETVGSIAIGYGDPPRDHQRLTEVAERY
                     GVEVERLRELSVNMPSRPQFIVAVAKARLRTEAVLLGEIIKRKGAEEALADLTKNLET
                     RIAKRTQQLRALATELNLAEQQERKRLAVELHDHLQHLLVLGKLRIGHGKRYAPPIPV
                     LTEALAQVDQVLSDALRYTRTLVAELSPAVLRDHGLLAALKWLSEYLQKLSVTVAVSM
                     PEELHITLPEDQAVLLFQSVRELLINSSKYAGTSAATVTVECRDDQLRIQVRDDGAGF
                     DVAAVTAAGSASTGGQSYKFGLSSILERMQALGGSFDILSAPGQGVTATLTLPLSQRE
                     TRPAA"
BASE COUNT         2965 a         3331 c         3444 g         2939 t
ORIGIN      
        1 caagcatcgc gaaggggatc aatggggttc atatgaaata ctctctggat gtcattccga
       61 ttgaagttct tgcactcgcc ctcattgagt ttatcttcgt gagtacgtat gccgcagtgc
      121 tttggtacgt gttaagtcac aaatgacccc gtggtattca tccgaaaggt ttagcgtgga
      181 caatttcctt ccatcggttt tcctatggtt cgcgcatcat cctttcggtg cggccacgat
      241 ggtgtatgca gccagcatcc tcggcgtctt gctgtatgcc tatttcgagc ctagggaccg
      301 tgtgtaagag cccctcaagc atcgctatta tcatggaaat gtgggaacag aggagagtca
      361 acctaagccg tatatgatgc ttccggttta tttctagcgg tcgtgagcac ttcgcggtca
      421 gtcccggcgt gagccgtcaa tcaatgatct ctctgggaga aaggacaggc aacaaggcct
      481 gatcgtacac cagtgtgaag gcaagggtct gtggcggcgc tgctcgcatc agtactgtag
      541 cgcgatcgtc atgtttaagc gttctccatt ataggagaaa cttgctgcct caaacgaggg
      601 cgctcccaac gtgcctttcg catcgttgga aaatccgtat ccctcactgg gcttccccat
      661 ccaattggtt gcgagttcgc catcccgatt ttcgtcatga atcaccgcaa gtgcatatgt
      721 gccaggcgct atgtctgcaa agtcgcacgt ggctttggtc gcgcggacct gaaccatcat
      781 aatattggtt gcaaaacgca agaactcagt aggaaaccct tccggccctt ggaacaatgc
      841 gcatgccaca gctcctatgc tattccggat gccaagaatc tccacgtgta accccggctg
      901 agatgcctga gcggaaccga tcatacccgc gagagcgatc aatatgagtg cccccacgcg
      961 ggctgatcgt agaatggata tcggcgtgcg ggtctgtctg cgcatacgat aacttgtcca
     1021 ggtcaagaat attgtcttga ccctcttctc cctggtccac cctgtataac cctaggtaaa
     1081 cgatggttct agcggacagg cagctgagtc acacgatgga acgcggcctc ggaggagggg
     1141 gcttacgatc tatcaagtta aacctcactg aaggcctcgc cgcgattgag gaccgcacct
     1201 ggtcctcgat gcacactcct cagtaaaaga ccttctgttc tgctccatcc cagcacgctg
     1261 gaatgtcaaa actaaacgtt aatgccctct tacctgctag ttcagtgagc tattttgacc
     1321 aacttgccac gggccagccg cccatactgt agatcatcga acgtttagaa catgtgctcc
     1381 attcgcttag gaaggaagag gtgattcaca tgcagtcaac ctatgaacag tggtcgcaag
     1441 gcaggagtca tatacagtgg ggagcgatcg ttgccggtct ggccgtcggt cttgccgtgc
     1501 aaatgctctt taccttgctc ggactggcca tcggagcgtg gtcaatcgat ttacgagaag
     1561 ctcaacccgc aggcggtatc ccattagcga ccggtctctg gaccggtatg tccatgttga
     1621 tgtccgcctt cgtcggaggc tatgccgctt cgcgtctgtc cggagcctcg aacaggacgg
     1681 agggcctgta tcatggcgcc gtactctggg gcgtcacgtc agtagttttc gcatggcttg
     1741 gcacgacggc gctgtcattt atgatcggag gactctttac tacggtgggt ggtgggttac
     1801 agatgctaag ccaaggcgtc ggcacggcag tctctgcggc ctcggcgaaa ataccgggta
     1861 ccggcatcac caacctcaca gcggccgacc ttcgcaatca gatcgaatct gttttgaaag
     1921 cgactgagaa gccagaattg cagcccagca cagtgagcaa ggatatagac aagaaaagta
     1981 ctcaggcgcg agagggccaa tcggttcaca aattgagcga ttcgaccatc ggggaaattc
     2041 gggacaaatt agtggccatg gaccgcgacg cggccgtaaa cgtcatggtg aacaaactag
     2101 gcatgtcgga gaaacaggct caagaagtgg cccaaaccgc catcggtatg gtggcttccg
     2161 tcaaagagac tgggaaggaa ataaaagaga aatccatgga tatcggtaat gccacgatga
     2221 cgggtgttgg atctgcagcc tggtggcttt ttgttttggc gttgctttcg cttggcgcat
     2281 cccttacggg cggtgcgctg gggactggcg acgaaatttt aagccgcctg gaaggtaagg
     2341 catatccgct cgaggtaaaa cccgcgatga gtaactgatc catgaaccag cggacggcct
     2401 cggtcggcgg aaccgtcgtt gctctgccgg ccaggtcgtc cgcgagcggt agggcatgca
     2461 aagcgttact taaaaagata gacaggccga aggctcacaa gcgccacttt taaatttgta
     2521 gaggagggca tatgacacct cttttcacca agcagacaat ttggagcctc acaatattgt
     2581 tgagcggctt ccttgcagcg gcgcctattc aggctcagga ccgagagccc gcaaccggcg
     2641 ctccaggatc caacgcgatt acggagtcgg acatcgcagc tggttcagtt gaggacacta
     2701 tcgaggcctg caaggccagg atacccaaag atgccagtat cggtcaggtc atgattgcgg
     2761 aacaaagttg ttggcgcgat gaaaatgaac gcaatcctgc gcaggcaatt cccggcgcac
     2821 gcagcaccag cctgaagggt ggtggctcgg tggcgatggg taccgagggt cgtcactgac
     2881 ggaggacggg ctcgatcgcg tagaggataa agagcagccg caaaaacaaa cggagtgcta
     2941 ttgcattttg cagtacatct tcagtctcag gcgatctctc ttttgagcaa ttttgaccag
     3001 actcgcatcc cacgtatctg gtacgtctat tctcgcgcct gcttcatcca tgacatatga
     3061 aggcggccac tgatgtgggg ccgttgtcct agagccagca aggaccctgc ctcgctttgt
     3121 accaatatag gtgcctgttg gaccacggag agtgatcatg gatcttattc gaccagtacg
     3181 accatcccga tcaacagaaa gtccgccacg acgcctactc ctggtggatc cctatccgct
     3241 caataatccc tatcacctga cggcatcgga acgccgagcc gtatggttcc caaaactgag
     3301 tttgccagtc atcgctgcct atacacctga gtcgtggtcc gtcgatctcg tggacgaagc
     3361 tgtccaggag gtcgattttg ataatccctg tgacctcgtg gggatatccg tgatgacctg
     3421 ttacgctcct cgcgcctacg agatcgcaac ggagttcaga aagcggggca aaacggttgt
     3481 gttaggcggc gtccatccca cctattgccc cgaagaagcc ctgcagttct gcgatgccgt
     3541 agtctgtggt gaggcggagg acctgtggcc tgaggtgata gccgacttca tggcagggtc
     3601 gctgcagcgc aaataccaga tgacagcgtt tcccgcgttg gagaattaca agagtccgag
     3661 ggtggagctg ttgagccccg atgcctacat gacccgtcag tgcagtttta cgacacgcgg
     3721 ctgtcatttc gattgcgagt tttgcagcgt gtcccctttt aacggcaaaa ccacccggcg
     3781 ccggccggtc gaagaagtca tcaaagaatt aacccaggta aagcggtggg tctgcggaga
     3841 gttggtagac cgcatgtcga atgacacact ctggaacgcc tttctcacgg gattacgcat
     3901 ccgattgggc atcgaagacg gcagcatcgt ggcctttgtg gatgatctcc ataacagcaa
     3961 tcgggcctac tgccgcgagc tgtgggccgc tctgaaaccc ttgaatatta aatggggctg
     4021 tcaatccaca ttgtttttgg gcgacgacga ggacatggtg aagctggcag cggagagcgg
     4081 ttgcgtgtcg gtgttcgtcg gaatggaatc actcgatgaa ggcgccttag aggaaaccaa
     4141 caagcctttt aatcgagtgg acaagttcgc acaggagatc aagatgtttc atgatcacgg
     4201 cattatggtg aatccgggga tcgtcttcgg attcgatcat gacgacgaat cggtgttcga
     4261 gcgagccgtg gagtttctta ccaagaacca ggtggagttg gcatatttca atgtgctgac
     4321 accgttgccg gggacggcgc tcttcgatcg ctacactgca gccggccgta tctttgatca
     4381 cgactggggc cactatgacg ggaagcatgt cgtgtaccgt cccagtcgga tgacgcccga
     4441 gcgattacag gagggatttt attgggcgaa ccatcagttc tattccctgc cctccatttg
     4501 gaaacgcctt tcaggcacca gtcagcggtt tgtcccacga ctgctcatga atcgagaatt
     4561 tcgcaagtta gtgaaacgat catgtccgcc gggggcctta tcggtagtca cgacagctct
     4621 caaaatattg caggcaaagc tgccgacgtt acaagccgga catcttattc cgaacgcctt
     4681 gcatgtgaat gtgatgaaag aggagaacaa ggaaagtgaa gtctcacgga ccggaagtcg
     4741 ctggttgagc attaaggcaa aacagcatga tcaatttgcg gctctgtttg tagatctcga
     4801 gggaacgttg gatcaggcga ccacgaaaga attggtcaag cgcatcacgg aggcggcaaa
     4861 gcgtgcaaaa ctcgacatca tcatcaattt tgagcatctg aaggatgcga caccagaagc
     4921 tctgcgagcg ttgggcaata gtgagttgtg gcgaggtgtc gtgccgtacg ccaaaataca
     4981 ctaccgaaag ttcaagtccg cgttcgaggc atcgcttgcc gacctctcca tcaatctact
     5041 gtgtgaggac gattcccaat caaaagccaa ggcctgtgat tagttgaacg agcgcacaat
     5101 tgtcgttacc gcgaagccgc cttggccatc tacaatcagg cgcaatcgac ccttttgact
     5161 tagatagcac aaggtgtaaa agacctctct caaggtcaac tccggggtgc gtttggccac
     5221 ctcctcaatg cgggcaccag gagccagggc tattacgtta aggatgtggt caatgatgat
     5281 gttatccggt ttcatgctgt gcaatatcgc ttgcgcttaa actgttccta gatccagtgg
     5341 ggccgctgag catgacatcg gcacaaggag ggttgccgta gtcccttgct tcggtgccga
     5401 ttcaaaatcg aactgaccgt tcaaggtttt cattcgttcc ggatcgcgtg aaggccgaat
     5461 ctcgttcact tgcgctcggt tcatttaatt ccgcctgtga gaggacaaat gctaagagaa
     5521 gaactctaaa gatgtcgtat tagatctcag atgttaggac aatgctatga ggaaaactgt
     5581 tcagaataga tcagtccgat catctggttt tctgcacttt atctgctacc acgctgctac
     5641 aaggatagga caaaccaagg caaaccatag ccacttgagg agcgttgtgt tctgttattc
     5701 ctcatggggt tagattgtct gaaagaggct atccagattt tttaaacggc gggtcggtgg
     5761 gctaccgaga gtgtggagac tcgagaccgc gagagttgag gaagcgaagg acgagaccca
     5821 cttccgacag cccatcgatt attcgctcca tcgacggcgg ttcttgcttg gcttgcaaaa
     5881 ccctcatcac ggttcttgtt ccttttaaac tgcttgtgtt tcaagagtga taggagcggt
     5941 ccgatgttcg gcgttatgag aacgaaagac gtgtgactgc atgtcgggtt gaaggagcgc
     6001 ttagatcggt gcgatttttc tgcacatatc ggcaatctgg cgctggacac tcggccgcag
     6061 cttggtaaaa gccagcccaa actcgcgata accactccac tgcactgtgg cttcctccac
     6121 ggtaaaagga tttccttcct gaggatagat ccagcactcg agcgtggtgc caagaggaac
     6181 tcccacagga ctggagatgc ggcagcccga gggcgaaagg tctcggagct cgccctcacc
     6241 accgacgatc tggctcttcg aagaaaattg actcgagagg tggacggcca cgcgactatg
     6301 acgccgcttc tcttttcgaa cgttcacacc aggacatgcc cgattgattg accttggcga
     6361 atttccgcgg ttcaccgcgg tcctcgtccc ccaccgggct agctcgatcg tctctctata
     6421 cccagcaagt ttttgccacg tctggttcat caaagaagtc agctgcggct tgagttgtcg
     6481 ccataggttg gcaacgtccc cgtcctgatc aacaggcgat tgcggtccgg ctcccggatc
     6541 aaagggattc gaactcaatt cttcaattcg ggcatcgacg tcctgaaaat gcggctcctc
     6601 tcgtcgaatt cgacgataga ctaccaaggc ctcgaaatcc cgattgagtg attccaaagt
     6661 ttggccgagc aaatacagta tgtgcacgcg ttctctggtc gaaaaggcac ctgtttccaa
     6721 tgcctgacga aaagccgtga ccgcttcatc gtgacgcttc agacttttta ggcatagcgc
     6781 tgcctgcgca aaggcttttc ccgcttgttg ctgatctctc gtcgcttgtt ggaattcctg
     6841 aagcgccgaa tcaaaaatct ggactttctt cagcaacagc cctcgctcat aatgatcgac
     6901 acccgaggtt tcttctgaca tgctgacctc cgtagtgctg cgttaaaagg aaagtcagca
     6961 agaataaagc ccgggtacga atcctagtga gagcccaaaa acaatgagtt ggccttcacg
     7021 aacaaaaaac ggctgaccgc gaagtccaac aaaatggccg gaaacgtacg cgagccgagt
     7081 aacacagcac ttgcttcctc cgaacgcgca ggatgtctgc ccatcttctc aaaaacgttc
     7141 aagtcttcaa ctcatccggc caatcattag aaacttcggc gtctaagact taatcgtgac
     7201 cagtcgcttc actttttcct tatgtacatg atggctggca tgtggtcagt acgcgtccaa
     7261 gggtggcgta ggtgaggcga cgtactattg cttcgcctta ttctgcgcgt tagggcatga
     7321 acggcctctt gcgcaaacat gtcgaggggt gtgggtcatt gccaatgagc cgtcgtttcg
     7381 ccattgatgg ccgcaagccg gctctcaatg cggatcttca gccgattgac aaatgttgtt
     7441 atggcgccta gcctaacacc cccacacaac agatgccggc gcatcattga aggcatgccc
     7501 gccgacatcc tgtagcgggt ggttgtcatg tcggacgcca ctaccatact tctcatcgac
     7561 ggaaataccc aagagcgaga atactatgcg cattacctcc atgcggcatc agccacctat
     7621 gaggtggccc aagccgcaat gggtgggtct ggcctagacc tctgcgcgag gcaaccaatt
     7681 gattgtgtcg ttcttgaaat cgacttgccg gatatgtctg gctttgaagt gctggcaagg
     7741 ttagttccac gtacctatca tccggagata gccgtcatcg tacttactcg gctctctaat
     7801 cctttcttgt tggaactggc cataaagaac ggtgcattgg gcgcgtttca taagaaaacg
     7861 gtaagcgggg acatactcga caagaccatt cgtaacgcca ttacgaccgt gcaaacgctc
     7921 aacagtgtct caatgttagc tcgataacaa tccttctgat cattccccaa ccatatatgt
     7981 tccagccgac tggcagtccg cgctgtctgg caaccatccc gtccatgggg ccaccgaatc
     8041 ggtgaggtaa tattcaccgt cgaaccgata aactgcaggc cgctcctcga ggttggggac
     8101 ggcgtttttc ggcgatagaa tgagactcca ctgagaggat ggaaatttct ataatgcatg
     8161 aaaacaatca cagtactcct tgttcagacg ctcaactgcc aatcgacgaa ttcatctgtc
     8221 acgcatcaga gcatgtggag cagatgttaa aggcaggggt gccgcggtgc gaaattctca
     8281 cccgtcttgc aagggctggt gaggtcctgg ccggtccaga cacatcggtt tcaattctgg
     8341 tcatcgatga ggatggtttg ttgcgcaatg ggtcttcacc aaatcttcct ttggattatc
     8401 tgaacgcgat cgatcgtctg aaacctgatg cgaaggtagg cacgtgcgca gccgcggcag
     8461 caacgggatc ggtggtcatt acacccgatt tttttgccga cgataagtgg gcggagctgc
     8521 gtcacctgcc cagctcacta ggctatgttg gggcgtggag catgccgata ttggctcctg
     8581 gaggaccggt gcttggaacc tttggtacat atttccgagc ccgccggact ccgaccacag
     8641 tagagaaaaa gggcgtggaa cttctcgccg ctgccgccgc actggtactt acaaaagttg
     8701 aatcagagca aagtggcgac agctatcgtc catagaacgc gcgatccaac ctgcttgctg
     8761 ctcatcctcc tcccggttaa taaaacgtcc cagtcgctga gtcgttgatg gattcgactt
     8821 ctggtgcatc cgccgcaagc cggtgctgaa ccaggctgag ctgttcgacg gaccgcaatg
     8881 gctacctgag aagcgtgaag ccgatgctgg ttccaaggcg gcgatatgaa ctccgaaccg
     8941 gctcttttgt gacggcgaat gacctatctg aggtgtgcac ttttgtccaa aatacgtggc
     9001 gcaaatcctt gagttggcgt ccctcctttg tattcgtact agtttggcca gtgtgtttgc
     9061 catgaggatc accattttga ggggttgagc agcaagcaaa tgattcgtac agtaaatctt
     9121 gttcactgga gattcttcaa ccgatcgaaa tggcctctag accggaggga ccatcgatga
     9181 gcacaccata ttcacgcgat gcgctcaatt cccgtttggc agagcggttg cccgcccatt
     9241 atacggttca gttcgcgtat ccggaatacg cgggtgaggg cgtgacgctt gatgtgtcgg
     9301 ccggcggatt atgcgttatc accaaccgag aaattgcacc tggcgttgaa ttgtatgccc
     9361 gcctcgtgct cccagacaaa acccacttag atgttcagac cgctacggtg aaatggtgtg
     9421 aaaacgggca agttggtctc gaaattgctg agatggaacg acaagatgca ctaggcttac
     9481 tggagctctt gtctcgctta ggcggatcag caattaccta ttctaggaga taccatccgg
     9541 ggtctgtcac cataggatca ggtaaagaac ttggcagcta cattgtaggg gcaatggaag
     9601 gaagcccact gataaatgcg tttacctcaa tctcccgaca gttgcgggcg cagttaagtc
     9661 gacacgatgc acatgtgccc ggaatgtccc ggtagccgag tgcgcttgaa acccttatct
     9721 gccgaatagc gacccatcgt tttcacaata cccaggcact aaaattccca gcagtgaggt
     9781 tccggtcttt tggccgccgg caatgtgact gaattctcag gtggtacgaa tctaccaaac
     9841 cgcaggtgag cttccatccg accgaaatct ttgccccctt gcatatatag tagatacgac
     9901 aaaacacatg ggatgggaac gatgtctgct caaggttaga aatacctgaa caacctctaa
     9961 cccttcaggg gggattgagc ttcaatcatt caggcgccta tgcttgcttc ctcagaggct
    10021 cgcatgatca atgttctgct ggttgatgac tatcctatta tgcagcaact gctgcgtgac
    10081 attttgcaac ggtaccccga tatctgcgta gtcggtgaag cgcaagatgg tgaagatgcc
    10141 gttctccaaa gcatgaaact caaacctacg gtggtcatca ttgacatcaa tttgccgacg
    10201 ataagcggta tggaggcgac gaggttgatt aagcggcaaa gaccatccac cactgtcatc
    10261 gggctgacag ccggagcacc agagcaaatt gtgactgcca tgcaggacgc tggagcagcg
    10321 gccgtgctga gaaaagaaga tttgttaagt gtgctgtatc ccaccatgat agaagccatc
    10381 agaagctcag cgaaacccgc ctttgcatct gcttcggcca gccctaagca ctgatccttc
    10441 acacccgctg tcgtaccgcc atgtccatgt gaatctgacc ttcatgtgtg cgtgtccagc
    10501 acttcccacg tttgccatcg gtgaaccctt cagcaagatc aaaatttccg ctgtcgccaa
    10561 accaccatat cgtgaaaaag ctgtcttagc gatggcgttc tccagcggtt gtggaaaaaa
    10621 cagatcccat gtaggacgga tcctctagca ctaatcccag taggagatac ggcctctgtt
    10681 ctatattttt gagccctcca tcatgaagac ctcgggaacc atctgatcct caaggagtgg
    10741 aggagtgctt gccggccaaa atgcgccgaa gcagtacgac gctacttctg attgttttct
    10801 tcagcactcc gtttcgcttt ggctccgtcc attctctagt cgtgtgcctt ttgatagtgg
    10861 caggaatggt ttgattgtct accgaacgtt gaggtgttga gatgcgactc atgcctttcg
    10921 cgtccagcgg taaggaaacc ttccttggga aacgtccgca agggcgaaaa tatttggtgg
    10981 gttctctggt gctctcggcg cttgccatcc gcatgctgct cgcacaacga aaacaggcag
    11041 aaggggaact tagggctatc aggtggctgc tgcgggaagg gggaaagccc tttcaaatgc
    11101 caaagccgca gccctatggg gatctgactg agctcaacac tgaactgacc gttcttcgca
    11161 gggtcggaaa agaactcctc ataaatgtgg ccggtgatgc gctccacctc attggtacct
    11221 cgggtgccgt atgcgaaaag aacggagact acgcttcctg tcttgtggca tccgactggt
    11281 gtcgctttct agaccaggcg tcgcgcgagc agtgtgggac ggtagacaat cgggctgcct
    11341 tgaccagcgg ccggtggcac tgtcgagaat cagggcgaga ggcgtcgatc ctcgcgatgg
    11401 agacagggaa accggtcgac ataccctgca aaggtggaat caatttgtat gcggcaccaa
    11461 tcatggtcga tggagaaacg gtcggaagca tcgccattgg ctatggggac ccgcctcgag
    11521 atcatcagcg gctgacggaa gtggctgagc ggtacggcgt tgaagtggag aggctccgcg
    11581 aactgagcgt caacatgcct tcccgcccgc aattcatcgt ggcggtagca aaagcccgat
    11641 tgcgcactga ggctgttttg ctgggggaaa tcatcaaacg gaaaggcgct gaagaagcct
    11701 tagccgattt gacgaaaaac ctggaaacac gcattgcaaa gcgcacacag caactccggg
    11761 ccctggcgac tgaattgaat cttgccgagc aacaggagcg caagcggttg gctgtggaac
    11821 tacacgatca cctgcaacat ctcctcgtgc tgggaaaact gaggattgga cacgggaagc
    11881 ggtatgcccc gccgattcct gttttaaccg aggcgttggc gcaagtcgac caagtgttgt
    11941 ccgacgccct tcgctacacc aggaccttag tcgccgaatt gagtcctgcc gtactgaggg
    12001 atcacggact gctagcggct ctcaaatggc tgagcgagta tctgcaaaaa ctctctgtga
    12061 ctgtcgctgt tagcatgccg gaagaactcc acatcacctt gccagaggat caagccgtgc
    12121 tgctctttca gtctgtgcgt gaacttctca tcaactcctc gaaatatgca gggaccagcg
    12181 cagccacggt cacagtcgaa tgccgcgacg atcaattacg aatacaagtg cgcgacgacg
    12241 gcgccggctt cgacgtggct gccgtgactg ctgctgggtc ggcctcgacc ggtgggcagt
    12301 catacaaatt cggcctgtct agtatcctgg aacggatgca ggctttggga ggatcgtttg
    12361 acattctatc tgctcctggg cagggcgtca cggcgacgct gaccctcccg ctgagccaaa
    12421 gggagacgag acctgccgct tgactggttt gacgagagag cttgcggtgt tgaaaaattt
    12481 tgagcgaccg tggccttggt ggagcttagt ttcatccgac ctgttcccct cacgttgagt
    12541 ctcgacctca ctgttaacca gcacatgaca cctgagaatg ctcggccctg agacgagttc
    12601 ctaatgtctc gttctgctga acaaagccgt ctaacttttc agccgctctc aaacagtccg
    12661 acatgcgccg ttgtcccct
//
LOCUS       DASTQY010000215         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1433219, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000215 DASTQY010000000
VERSION     DASTQY010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433219"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VFR82_07130"
     CDS             <1..819
                     /locus_tag="VFR82_07130"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU4501409.1"
                     /translation="GHERSRKVDDILSEAATLATAGHHELVLTGVNIGQFSQSGVTLA
                     GLIDRLEGIHGIQRIRLSSIEPTTVTDDLLERMGASRKLCPYLHVPLQSGDDTILNAM
                     NRPYDIKAFISLVERATARVPDLTWGTDVMVGFPGESDSAFEHTLDVCRQLPFSYFHV
                     FTYSRRPGTAAAKMAGTVPVNVARLRAKTLADLSRDKRFAFAERWIGSSVAVLFESGT
                     IDGLRLGTTRHFLRVAVSSEGPLANQIHPVIITGASDRWAVGYILDRKGLDHES"
     gene            809..2215
                     /gene="miaB"
                     /locus_tag="VFR82_07135"
     CDS             809..2215
                     /gene="miaB"
                     /locus_tag="VFR82_07135"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEU4501410.1"
                     /translation="MNHKPGLRQIHIETFGCQMNEYDTELVRALLKKAGYVFTEDREC
                     ADVILMNTCAIREHAHTKVYTHLSDLRALKKSRPLVVGVLGCMAQNLKAELTKQEPMV
                     DLLAGPDSYRQLPILIDHALQAQEEGAHVKRLAVDLSEYETYEDIVPARVPGVNAWIA
                     VMRGCDNFCSFCVVPYTRGRERSREPEGILEEARRIAAEGFKQITLLGQNVNSYRSGN
                     WDFARLLAAVADIPGIERVRFTSPHPKDFPPALLEAVAFHPKICKHIHLPVQSGNDRI
                     LGLMNRSYTRRDYLALVEQIRSINPSIVLTTDIIAGFCSETEGEFTDTYRLLEEVRYH
                     SAFIFVYSERGNTIAARKFPDDVAAEVKSQRVTALVETQRRISSERNREYIDMTLPVL
                     VEGDAKKSPLQAMGKTDGHITVIWNKRDLPSRPGQVVSCSIYDASASTLYAKAPALSD
                     LHRPENEHSCTNSDHDVA"
BASE COUNT          544 a          684 c          684 g          531 t
ORIGIN      
        1 ggccacgagc gaagccgcaa ggtcgatgac atcctttcag aagccgccac actggctacc
       61 gccggccatc atgagctcgt tctcacaggt gtgaacatcg ggcaattttc tcagagtggg
      121 gttaccctcg ccggtttaat tgacaggttg gagggaatac acggcatcca gcgcatcagg
      181 ctttcgtcta tcgaaccgac gacggtgacg gacgatctgt tggagcgcat gggcgcgtcc
      241 cggaagctgt gtccctatct ccacgttccg cttcaaagcg gagacgacac gattcttaat
      301 gccatgaacc gtccctatga cataaaagca tttatctcgc tcgtcgagcg ggcgacggca
      361 agggttccgg atctgacatg gggaaccgat gtgatggtag gatttcccgg cgaatccgac
      421 agtgcttttg agcatacgct tgatgtgtgc cgtcagctgc ccttctccta tttccacgtg
      481 ttcacctact caagacggcc gggcactgcg gcggcaaaaa tggctggcac ggttcctgtc
      541 aacgtcgctc gattgagggc gaagacgctg gctgatctat cgcgcgacaa gcggtttgcc
      601 ttcgccgaac gatggattgg atcgtcggtg gccgtgctgt tcgaaagcgg cacaatcgac
      661 gggctccgcc ttgggacaac caggcatttc ttacgagtgg ctgtgtcgtc cgagggccca
      721 ttggcgaatc agatccatcc agtcatcatc acgggggcgt cagaccggtg ggccgtggga
      781 tacattctcg accggaaggg gctcgaccat gaatcataaa cccggcttgc gtcaaatcca
      841 tattgagacc ttcggctgtc aaatgaacga gtatgacacc gagttggtcc gcgccctgct
      901 taagaaggcg ggctatgtgt tcactgagga tcgagaatgc gcagacgtga ttctcatgaa
      961 cacctgcgca attcgggaac acgcgcatac caaggtgtac acgcacttgt cggatctcag
     1021 ggcactgaag aagagcagac ccctggtggt gggggtcctg ggctgcatgg ctcaaaatct
     1081 caaagcggaa ctcaccaaac aagagccgat ggtcgacctc ctggctggac cggactccta
     1141 ccgtcagttg ccgatactga tcgatcacgc cctgcaagcc caagaagaag gtgcgcatgt
     1201 gaagcgcctg gcggtggatt tatcggaata cgagacctat gaggacatcg ttccagcgcg
     1261 tgttccaggg gtcaacgcct ggattgctgt gatgcgcggc tgcgacaact tctgcagctt
     1321 ctgcgtcgtg ccctacactc gcggcagaga acgatcgcgc gagccggaag gcatacttga
     1381 ggaagcaagg cgtatagcag cggaaggttt caaacagatc accttactcg ggcagaacgt
     1441 caactcttac cggtcgggta attgggattt cgcacggttg ctggccgccg tggcagacat
     1501 ccccggaatc gaacgcgtga ggtttacatc tccccatccc aaagatttcc cgccggcctt
     1561 gctggaggct gtggccttcc atccaaaaat ttgcaaacac attcacctac cggtccagtc
     1621 aggaaacgat cgcattctgg gcctgatgaa ccgctcctat acgaggaggg actatcttgc
     1681 gctcgtcgag cagatccgat cgataaatcc gagcattgtc ctgacgacgg atattattgc
     1741 cggcttttgc tcggaaaccg agggggaatt tacagatacc tatcgcttgc tcgaagaggt
     1801 tcgctatcat tccgccttca tcttcgtgta ttcggaacgc ggcaatacca tcgcggcgcg
     1861 aaagtttccg gatgacgttg ccgctgaggt caaatcccaa cgtgtcacgg cgctcgtgga
     1921 gacgcagcgc cggatctcca gcgaacgcaa ccgagaatat atcgacatga ccctgccggt
     1981 gcttgtcgaa ggcgatgcca agaagtcacc actgcaagcc atgggaaaaa cggacgggca
     2041 catcacggtg atatggaaca agcgtgacct gccttctcgc cccggccaag tggtttcgtg
     2101 ctccatctac gacgcatcgg cttcgacgct ttacgccaaa gctccagcgc tttctgatct
     2161 gcaccggcca gaaaacgaac actcctgcac caatagtgac cacgacgtag cttaatgcat
     2221 gcggtgcgta tttcttctcg agcgccgctg gacgcttgcc tcagcagggc gaatctgccg
     2281 gtgcctcggc aactgagtat aaaacttctg ccgcttcttt catttacggt caattagatc
     2341 aaagtcttat acagcgtccg tttgcgcggc ggatcgacag gcggtcctcc acttgtaaga
     2401 ggattcgttt tccttttaac cgttcaaggt aaggattgcg cat
//
LOCUS       DASTQY010000216         3859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1435048, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000216 DASTQY010000000
VERSION     DASTQY010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435048"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="VFR82_07140"
     CDS             <1..1025
                     /locus_tag="VFR82_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4501411.1"
                     /translation="FKATDSQAILMQRTDELVSKYVQTRQTRFGILIRQYLGSVGWQA
                     IAHGGLIATAAWLLSIGQLTLGQLVAAEVVVSGLLASFDEVVKRMGHIFYFMTGLREL
                     DLVLSLPKDQGSSALSVPLPDPTVHGIRLTCKDLAVHHAGVPAIFEQFNVEVTPGEKI
                     GIYASTTAAKTALARVLAGLEMPTGGVIRYNGVDLRHIDMNAINRCRGFMIDSQLTLF
                     EGTIEDNILLGRSYIPYSDVRWALRFAELEEDVDALPQGLKTHIRAPGKILAPTHMMR
                     ILLARAILGRPQILIFDGILHTLQPVLRETLLRRLCSKDEPWSVIFVSNDPNLTPHVD
                     RRLILE"
     gene            1036..1821
                     /locus_tag="VFR82_07145"
     CDS             1036..1821
                     /locus_tag="VFR82_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HEU4501412.1"
                     /translation="MLLLRWLRSFRPSASQVLISLLIAGLGWLSGGALSRVDQDLRIM
                     YTEYTLGAADLAHISADVMRYRNTIIRALEAGSRKDFERITESLPGQRARVQLAVDRY
                     AAAGLRVSRSGRSEPEDIEAVRKSLDQYFSAASTTIDLLSREWNASSPSERDRLRRNA
                     EEHAADNAGPKMIQVSLALDRLLDTVADVAKDMRDEGTKAIQSTSVMLVGGSFFIAVL
                     NLFLTRPRTPGGQEAGRSPESLAEPLRPTLPIRSGNQHHPLRS"
     gene            complement(1845..2561)
                     /locus_tag="VFR82_07150"
     CDS             complement(1845..2561)
                     /locus_tag="VFR82_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU4501413.1"
                     /translation="MDKIRINKFFTEHGICSRREADRLIEAGRVTINATVAKLGDQVS
                     TGDVIACDGRVISWGQTRVYIKYHKPVGVTTTSESHIPRNIISEIGHPERIFPIGRLD
                     KDSSGLILLTNDGDIVNEILRTEFGHEREYEVGLDRPYDQSFLDRLAKGVVILGRKTQ
                     PCRVQRLDPKRFKIILTEGRNRQIRRMCQALGYRVVALHRVRIMHVTLTGLPSGRWKP
                     LTPEEQRELFRAVRRSSARD"
     gene            complement(2705..3637)
                     /gene="speB"
                     /locus_tag="VFR82_07155"
     CDS             complement(2705..3637)
                     /gene="speB"
                     /locus_tag="VFR82_07155"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEU4501414.1"
                     /translation="MTLPAGWEDSQHNFLGLEEPWCHPDQAGVYVLPAPYEHTSSYIL
                     GSDEGPSAIIEASHQVECYDETLRCEPFREWGGIATVAPLELKGKIDREAVDAIEQFV
                     HSHIGNGRFLVTLTGEHTGALGAIRAHAKRYPNMSVVQIDAHGDLRHAYQDNLYSHAS
                     VMARVVDDGLPLVQVGIRSICPEEIERIRCTDRIATFFAAELLDPSGPYEGKASRWIP
                     QVVKACQGPVYLTFDCDGLDASLVPALGTPEPGGLGWYDTLNLITALSNGPGILGMDI
                     SEIAPIEGFVAPQFCVARLIYRILGRIKAGRRVH"
BASE COUNT          800 a         1163 c         1092 g          804 t
ORIGIN      
        1 acttcaaagc cacggatagt caggccatcc tgatgcagcg gaccgacgaa ctcgtcagta
       61 agtacgtcca gacaagacag acccgattcg gcattctcat ccggcagtac ctggggtcgg
      121 tgggctggca ggccatcgca cacggcggat tgattgccac tgcggcttgg ttgctgtcca
      181 tcggccaact caccttgggg caacttgtgg cagccgaagt ggtcgtcagc ggcctattgg
      241 ccagttttga cgaagtcgtc aagcgaatgg ggcacatatt ctacttcatg acgggattga
      301 gggaactgga tctagtgctg tcgctcccga aggaccaagg ctcgtccgcc ttatcggtcc
      361 cgcttcccga tccgaccgtg cacggcatcc ggttaacgtg caaggatctc gcggttcatc
      421 acgcgggcgt cccggcgatc ttcgagcagt tcaatgtcga agtcactccc ggcgaaaaga
      481 ttggcatcta cgcgagcacg acggcggcga aaacggccct ggcgcgcgtc ctagccggcc
      541 ttgagatgcc gactggcggc gtgatccgct acaatggcgt ggacctgcgg catatcgaca
      601 tgaatgccat caaccggtgc cgcggcttca tgatcgattc ccagttgacg ctgttcgaag
      661 ggacaatcga ggacaatatc ctgctcggcc gttcctatat tccctacagt gatgtccggt
      721 gggcattgcg ctttgccgaa ctggaagagg acgttgacgc cctcccgcaa ggcctcaaaa
      781 cgcacatccg ggcgcctgga aaaattctgg cccccacaca catgatgcgc atcttattgg
      841 ctcgtgcgat tctagggcgc ccgcagatcc tgattttcga cgggattctt cacacactcc
      901 aacctgtcct gcgtgagacc ttgctccggc ggctctgttc caaagatgag ccctggtcgg
      961 taattttcgt gtccaatgat cccaacttga cgccgcatgt cgatcggcgg cttattttgg
     1021 agtaaggaga caaccgtgtt gctcttgcgg tggctacgct cgttccgtcc cagcgcttcg
     1081 caggtcttga tcagcttgct gattgcaggg ctcggctggt tgagcggcgg cgcgctcagt
     1141 cgggtggatc aagacctgag gatcatgtat accgaataca cgctcggcgc cgccgatctg
     1201 gcacacatct cggcagatgt catgcggtac cgcaacacca tcattcgagc attggaagcc
     1261 ggcagtcgaa aagacttcga acgcatcacc gagtcacttc ccggtcagcg ggctcgggtg
     1321 caactcgctg tcgatcgcta tgcggccgca ggccttcgtg tttctcgcag cgggaggagc
     1381 gagccggaag acatcgaagc cgttcgaaaa agcctggatc agtacttttc tgcggccagc
     1441 accacgatcg acttgctgtc cagagaatgg aatgcctcct cacccagcga acgggatcgg
     1501 ttacgccgca atgcagagga acatgccgca gacaatgccg gccctaagat gatccaggtc
     1561 agtctggcac ttgaccgatt gctggacacg gtcgccgatg tggccaaaga catgcgtgac
     1621 gagggcacaa aagcgattca atcgacgagc gtgatgctcg ttggcgggag cttcttcatc
     1681 gcggtgctga acctcttctt gacccgcccc cggactcccg gcggacagga ggccggccgg
     1741 tcgccagaat cgcttgctga accgttgcga cccacgttgc cgattcgaag cgggaatcaa
     1801 caccatccgc tccgctccta gggcggttca cgcccatcat cctcctagtc gcgtgcggaa
     1861 ctccgccgga ccgcacgaaa gagttcccgt tgttcttcgg gggtgagggg tttccacctc
     1921 cccgacggca gaccggtcag cgtcacgtgc atgatcctga ctcgatgcag tgccaccacc
     1981 cggtacccca gcgcctgaca cattcgccgt atttgcctgt ttcggccttc agttaatatg
     2041 atcttgaatc gtttcggatc caggcgctgg acgcgacaag gctgggtctt ccttccaaga
     2101 atgacgacac ccttcgccag gcgatcgaga aaagactgat cgtagggccg gtcgagcccc
     2161 acctcgtact cgcgctcgtg accgaactcg gtgcgcaaaa tctcgttgac gatgtcacca
     2221 tcgttggtca gtagaatcaa gccggaggaa tccttgtcca gccggcctat ggggaaaatc
     2281 cgttccggat ggccgatctc actgatgata tttcgtggta tgtgactttc actcgtcgtg
     2341 gtgacaccca ccggcttgtg atacttaata tatactcggg tctgccccca ggagatcacc
     2401 cgcccatcgc aggcaatcac atctccggtg ctgacctggt cgcccagctt ggcgacagtg
     2461 gcattgatgg tgacccgtcc cgcctcgatc aatcgatcgg cttcgcgccg agagcagatg
     2521 ccgtgctcgg tgaagaactt gttgatgcgg atcttatcca caacccaatg gaagactatt
     2581 gcgcaggatg ctcacaacga ccgctcagca aggccgcaac gggcgactga cccgaggcgt
     2641 acttttgttc ggtaggttga gggtcgagcg atgtgaggat gcagctggca ttttcagcat
     2701 cctgtcagtg gactcgacgg cccgctttga tgcggccgag gatgcgataa atgagtctcg
     2761 cgacacagaa ttgcggggcg acgaaccctt caatcggagc aatttcgctg atgtccatgc
     2821 caagaatccc cggcccgttg gaaagtgccg tgatgagatt caacgtgtca taccagccca
     2881 acccgccggg ctccggtgtg ccgagcgccg gcaccaacga cgcatcgagg ccatcacagt
     2941 caaacgtcag atacaccggc ccctggcagg ctttcacgac ctgaggaatc cagcgactcg
     3001 ctttcccttc gtacggaccg gacggatcca acaattcggc agcaaaaaaa gtggcaatcc
     3061 gatcggtgca tctgatgcgc tcgatttctt ccggacagat cgatcggatg ccaacttgca
     3121 cgagcggcaa tccatcatcc acgacgcgcg ccatgacgct ggcatgactg tacaaattat
     3181 cctgataggc atgccgtaga tcgccgtgcg cgtcgatttg aacaacgctc atgttcggat
     3241 accgcttggc atgcgcacga atcgccccca gcgctccggt gtgctcgccg gtgagtgtca
     3301 ccaaaaaacg gccgttgccg atgtgggaat gcacaaactg ctcgatcgca tccaccgcct
     3361 cgcggtcgat tttgcctttg agctcaagcg gtgcgaccgt ggcgattccg ccccactccc
     3421 gaaagggctc gcagcgaagc gtttcgtcgt aacactctac ttggtgagaa gcttcaatga
     3481 tggccgaagg accttcatca gagcccagaa tgtagctgga cgtatgctcg tagggtgcag
     3541 ggaggacgta cacacctgcc tggtctggat gacaccaggg ctcttcaaga ccgaggaaat
     3601 tatgttggga gtcttcccat cctgcgggaa gcgtcatgga tatgtactcg caggccgcga
     3661 gagaacccac tgcgccgccg gaccttggca gtcccgatca acgacgggcc cgcttcggca
     3721 gattctccag agggatgaac acagccaacg ccacgacggt tgtccatcgc ccgggctttc
     3781 caagtgccga ctgcgtgatg ttcagagtcc ggacgatctt tccacccatc ttaaacactt
     3841 gttctcgttc tttccaagc
//
LOCUS       DASTQY010000217         3593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1123647, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000217 DASTQY010000000
VERSION     DASTQY010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123647"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1631)
                     /locus_tag="VFR82_07160"
     CDS             complement(123..1631)
                     /locus_tag="VFR82_07160"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4501415.1"
                     /translation="MESATQDHPATTPSSPSPVSDPAPTGPAPTARIRLSEQARRLAT
                     WIGHWSIEQRVLAGFGLVFLGILVISVMSYRNTAVVVKNSQLDTASHELIQLLGSIEE
                     ALVAAESGHRRYLVTGDDSYLKAYRGIMEQMPEFSRYLHALAGASPEQRERVSQLEQL
                     IKQQLDIETNAIRERDTRGAEAVRQLALPGVAKRELDNIHRLIADFESTAQQATGARV
                     LQSAGTTRSTIALLALGAVLQLVLLGSVYYLIHHDITNRRRVAAELRRQGELLQAANK
                     ELEAFSYSVSHDLRAPLRHIDGYAALLSKAAGQALDEKAQRYLQTISDSAKQMGQLID
                     DLLVFSRMGRQEMLRTTVNLNQLIKAVIQDLRLDLQGRTISWRIGALPDVPGDPAMLR
                     QVFMNLLTNALKFTATRQNGQIEIGAKREVPGEVTVFVRDNGVGFDMQYVHKLFGVFQ
                     RLHRNDEFEGTGIGLANVRRIVHRHGGRTWAEGALDQGATFYVALPLKRTSS"
     gene            complement(1751..3253)
                     /locus_tag="VFR82_07165"
     CDS             complement(1751..3253)
                     /locus_tag="VFR82_07165"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEU4501416.1"
                     /translation="MTFTLTMSPSDLLLLLPEIFLTCWLCLVLIVDFSFPRLPKEHLA
                     YLSIAGLLGTLGCLAWFDFSGISGALFANMFVVDRMALFFKIFIVAATILVILASIEF
                     IHRFTFFRGEYYFLVIMSALGMMFMASANDLLSVFVTLEFSTFGFYVLVSYLREDVAS
                     NEAGLKFFILGVFAAGLLAYGISLVYGETGKLVFSDMKGAQATPGLVIGFLLIFGALG
                     FKIGAVPFHSWIPDTYHGSPTPVTAFLSIAPKGAAFAILLRMFFVALGTFKPTWVLLL
                     VAASILSMTYGNIVAIAQRNMKRLLAYSGIAQIGNVLIGLAAGTKMGNDAILFYLLTY
                     LFANLGAFAIIIAVSHAIGSEEIDDYSGLGRRSPFLAFSMLIFLLSLAGVPPLAGFIG
                     KLYIFIAAVKEGLYTLITVGLINIVISMYYYLIVVKKMYITEPHDPSRVTVSGPMKAV
                     IYVGLAGTLIIGIYPQPVIDWVVAATMMFADLGGQAAGVPNMTSLLASGH"
     gene            complement(3250..>3593)
                     /locus_tag="VFR82_07170"
     CDS             complement(3250..>3593)
                     /locus_tag="VFR82_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501417.1"
                     /translation="AYLFKMMRGLFYGPMDQKYSHSHDAVAVVDRLPLLIMIVVSIGF
                     GLFPMHMYNVVRSGVDPLVAKITLVVPVADARDEQAVPRERTSPATISPSPLVATKPG
                     QAPPQAVTGLP"
BASE COUNT          861 a          988 c         1048 g          696 t
ORIGIN      
        1 cacctctgcg ccgtcatgac agacgaccac tttgtcggca atgtgatgct cctccatggc
       61 ggcgagcgcc aattcggcat cccgaggatt gtcttccgcc agcaggatcg gtttcacagg
      121 agtcatgagg acgtccgttt caagggaagg gccacataaa atgtcgcacc ctgatcgagg
      181 gcgccttcgg cccaggtccg gccgccgtga cgatgcacga tccgcctgac attcgccagc
      241 ccgattcctg ttccctcgaa ttcatcgttt cgatggaggc gctgaaatac gccgaacaat
      301 ttgtgcacgt attgcatatc gaagcccacg ccgttatcgc ggacaaacac cgtcacttcg
      361 ccagggacct cccgcttcgc gccgatctca atttgaccgt tctgcctcgt cgcggtgaat
      421 ttcagggcat tcgtcagcaa gttcataaac acctgtcgca acatggccgg gtcgcccggc
      481 acgtccggca atgcgccaat tctccaagag atcgtccttc cttgcaagtc aagacgtaaa
      541 tcttgaatta cggccttaat cagctggtta agattgacgg tcgtgcgcaa catttcttgg
      601 cggcccatcc gcgaaaatac aaggagatcg tcaatcaact ggcccatctg cttcgcggag
      661 tcggaaatgg tttgcaggta gcgctgggcc ttctcatcca aggcttggcc cgccgccttt
      721 gacagcagcg ccgcataccc gtcgatatgc cgtagcggcg cgcgaagatc gtgtgagacc
      781 gaataactaa aggcttccaa ctccttgttc gcagcctgaa gcagctcacc ctggcgtcgc
      841 aattcggccg ccacgcggcg tcgattcgta atatcgtgat gaatgagata gtagactgac
      901 cccaggagaa cgagttggag cacggcgccg agtgcaagca gtgcaatggt gctgcgggta
      961 gttcccgccg actgcaatac ccgggcaccg gtcgcttgct gcgccgtcga ttcgaaatct
     1021 gcgatgaggc gatggatatt gtcgagttcg cgttttgcca caccgggcaa tgccagctga
     1081 cggacggcct cagcaccccg ggtgtcccgc tccctgatgg cattcgtctc aatgtccagt
     1141 tgctgcttga tgagctgttc cagctgagag acgcgctccc gctgctcagg cgaggctccg
     1201 gcgagggcat gcagataccg cgaaaattcg ggcatctgct ccatgattcc gcggtaggcc
     1261 ttgaggtagg aatcgtcgcc ggtgaccagg tagcggcgat gaccgctttc ggccgcgacc
     1321 agcgcttcct caatagaccc cagcaattgg atgagttcat ggctggccgt atccagctgg
     1381 ctgttcttca cgacgacggc ggtattgcga tacgacatca cgctgatgac gagtattcct
     1441 aggaagacca gtccgaaacc agccaggacg cgctgttcga tcgaccaatg accgatccag
     1501 gtcgctagcc gtctagcctg ttcggatagg cgaatgcgcg cggtcggtgc cggtccggtc
     1561 ggtgccgggt cggagacggg ggaaggagat gacggcgtgg ttgccggatg gtcctgggtg
     1621 gcggattcca tgggttcgtg gcagcacgaa ggtcgaaata gtatgaaaac ctaacaaact
     1681 ctactctggc cgattcagcg ctgcaagcaa tatttcagat gactaacggg ggaagggaag
     1741 gaaaaacaga tcagtggccc gacgccaaga gcgaggtcat atttggcact ccggctgctt
     1801 gacctcccaa atcagcgaac atcatggtgg cggccacaac ccagtcgatc acgggctgag
     1861 gataaatacc gatgatgagc gttccggcca agccgacata gatcaccgct ttcataggac
     1921 cggagacggt cacccgggat ggatcgtgag gctccgtaat gtacattttt ttgacgacga
     1981 tgaggtagta atacatcgag atcacgatgt tgatgagccc caccgtaatg agggtataca
     2041 gtccctcctt gacggctgca atgaagatat acagtttacc gatgaagccc gccaaggggg
     2101 gaacgcccgc cagagacaac aggaagatca gcatcgagaa ggccagaaac ggcgatcggc
     2161 gtcccaaacc gctgtagtcg tcaatttctt cactgccgat ggcatgactg acggcgataa
     2221 tgatggcaaa ggctccgaga ttggcgaaga ggtaggtcag tagatagaac agaatcgcat
     2281 cgttgcccat cttcgtcccg gcggccagac cgatgaggac attgccgatt tgcgcgatgc
     2341 ctgagtaggc caacaatcgc ttcatattcc tttgcgcgat ggccacgata ttgccgtagg
     2401 tcatggagag gattgatgcc gcaaccaaca gcagaaccca cgtgggtttg aacgtgccaa
     2461 gggcgacaaa gaacatgcgc aacaagatgg cgaacgctgc gcctttcggg gcgatggata
     2521 agaaggccgt gaccggtgtc ggtgatccgt gataggtgtc gggaatccag gaatgaaacg
     2581 ggacggcgcc gatcttgaat cccagcgcgc cgaagatgag caggaacccg atgacgagtc
     2641 ccggcgtggc ctgggcgcct ttcatgtccg agaagaccag cttgccagtc tctccatata
     2701 ccaagctgat gccgtaggcc agcagacctg ccgcgaacac tcccagaatg aagaatttca
     2761 gtccggcctc attgctggcc acatcctcgc gcaaatagga aaccaggaca taaaagccga
     2821 acgtcgagaa ttcgagcgta acaaatacgg acaggagatc attagccgag gccatgaaca
     2881 tcatgcccaa ggccgacatg atcacgagga agtaatattc accacggaaa aaggtaaacc
     2941 gatggataaa ttcgattgaa gccaagatga cgaggattgt ggcggcgaca atgaagattt
     3001 tgaaaaacag cgccattcga tcgactacga acatgttggc gaacagcgcg ccggaaatgc
     3061 cggaaaagtc gaaccatgcc aggcagccga gtgttcccag gagacccgct atgctcaaat
     3121 acgccaaatg ctccttgggg agccgtggga aggaaaagtc gacaatcaag accagacaca
     3181 gccagcaagt taggaatatc tcaggcagca acaggaggag gtcggagggg gacatggtca
     3241 gcgtaaaggt catggcaatc ctgtcacagc ctgaggcgga gcctgccctg gtttggtggc
     3301 gacgagggga ctcggcgata tcgtcgccgg gcttgtcctc tctcgcggca cggcctgctc
     3361 gtcgcgcgcg tcagcgaccg gcacgaccaa ggtgatcttg gcgaccagtg ggtcgacgcc
     3421 ggaacgcacg acgttataca tgtgcatggg aaagagaccg aagcctatgc tcaccacaat
     3481 catgattaat aatgggagtc gatcgaccac tgcgacggcg tcgtgcgagt ggctgtattt
     3541 ttgatccatc ggcccgtaga agagcccgcg catcatcttg aacaaatacg cca
//
LOCUS       DASTQY010000218         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_216773, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000218 DASTQY010000000
VERSION     DASTQY010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216773"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFR82_07175"
     CDS             <1..522
                     /locus_tag="VFR82_07175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4501418.1"
                     /translation="LESLDINQVATLVVESLRTVAAEKQVEIRVEAPEQLPMIHADRD
                     KIEQILWNLIGNAVKFTPPAGLVTVRFDALPDGLIQTCVSDTGCGIPSDHLDRVFHEF
                     SKVPSTMPASQGAQLGLFITKSFITMHKGEIRVDSELGVGSRFFFTMPAVAPGAGGIS
                     PQIVSPGSSAKTA"
     gene            616..2076
                     /locus_tag="VFR82_07180"
     CDS             616..2076
                     /locus_tag="VFR82_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU4501419.1"
                     /translation="MRARILIVDDDPDIVLALENRVTWMGHEPLTAANGKEALRLIQS
                     EQPDLVLLDIQLPVFSGLEILQQMHDSVQKPTPSDPAGSSMPPTVMLTAFGTIELAVK
                     AMQLGAVDFITKPFTGDHITVVINKALDAIALRRQVGVLKQELEDRYGPIVGSNVKFT
                     TQLALAEQAAASNVTVLILGETGTGKEVVARAIHGWSPRKQKPFVAVNCAAIPKDLLE
                     NELFGHEKGAFTGAIKREPGKIEIAEGGTVFLDEIGDMSLSLQSHLLRVLQDQTFYRV
                     GGTQSVHTDVRFIAATNKDLKQAIRQGTFREDLFYRLAVISVILPPLRDRIDDIPALA
                     GYFLKRAVKLGLSKQCNMSDAALHTLAQYNWPGNIRELENVLTRAYILSPGKTIEPEH
                     LCLSESVIACDPIEAQPLQHRPMAGNGYHEMMEAYSRWVLEEALKKNDWNQTRAAEAL
                     GLQRTYFTKLLRQKQIPGRQPKPASPSSSSQADMNH"
     gene            2158..2233
                     /locus_tag="VFR82_07185"
     tRNA            2158..2233
                     /locus_tag="VFR82_07185"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2191..2193,aa:Met,seq:cat)
BASE COUNT          657 a          805 c          800 g          605 t
ORIGIN      
        1 ctcgagtcat tagacatcaa ccaggtcgcc acgctggtcg ttgaaagctt gcgaacggtg
       61 gcagcagaaa agcaagtgga aatccgcgtg gaggcgcccg agcagctgcc catgattcat
      121 gctgatcgcg acaagataga gcaaatcctc tggaacctca ttggaaacgc tgtgaagttc
      181 acgccacccg cgggattggt gacagtccgg ttcgacgcct tgccagacgg cctgatccaa
      241 acctgcgtgt cggacacggg gtgcggaatc ccgtccgatc atctggatcg ggtgtttcat
      301 gagttctcaa aggtgccttc tacgatgccg gcgtcgcaag gcgcccaact tgggctgttt
      361 attaccaaga gcttcatcac gatgcataag ggagagatcc gggtcgatag cgaattaggg
      421 gtaggttcgc gctttttctt taccatgccg gctgtggcgc caggggcagg tggcatctcg
      481 ccccaaatcg tctctccagg ctcgagcgcg aagactgcct aggcgcgtcg aggggacacc
      541 tgtctatgct ttgtcggaga cactcggcat gttgacgtcc aggacttttc agtcatctgc
      601 tgaaaggaac gaaccatgcg cgctcgaata ctgatcgtcg acgacgatcc ggacattgtg
      661 ctggcattgg agaaccgcgt cacctggatg ggacacgaac ctttgacggc cgccaacggc
      721 aaggaggccc tgcgcctcat tcaaagcgaa cagcccgatc tggtgctcct ggacattcag
      781 cttccggtct tctcggggct tgagattctc cagcaaatgc acgactccgt gcaaaaacct
      841 acgccatcgg accctgccgg ctcttcgatg ccaccgaccg tcatgctgac cgccttcggg
      901 acgattgagt tggcggtcaa ggcgatgcag ttgggtgcgg tggatttcat caccaaaccg
      961 ttcaccggcg atcacatcac ggtcgtgatc aacaaggcgc tggatgccat tgccttgcgc
     1021 cgacaagtgg gcgtcctcaa gcaggaactg gaggaccgct atggtcccat cgtgggcagc
     1081 aatgtgaaat tcacgacgca gcttgcgttg gctgaacaag ccgccgcgtc caacgtcacc
     1141 gttctcatcc tgggagagac gggcaccggc aaggaggtcg tggcgcgcgc catccatgga
     1201 tggagccccc ggaagcaaaa accatttgtt gccgtaaact gtgcggcaat cccgaaagac
     1261 ttgctcgaaa atgaactgtt cggccatgaa aagggcgcgt tcacgggagc catcaagcgg
     1321 gaaccaggga aaatagaaat cgcagagggc gggactgtct ttttggatga aatcggcgac
     1381 atgtcgctgt ccttacaaag ccacttgttg cgcgtgctgc aggatcaaac gttttatcga
     1441 gtgggcggta cgcaatcagt tcataccgac gtgaggttca tcgctgcaac gaataaggat
     1501 ctgaagcagg ccatccgaca ggggacattc agggaagacc tcttctatcg gctcgcggtc
     1561 atttccgtca tcctcccgcc gcttcgagac cgcatcgacg atatcccggc tttagcaggg
     1621 tatttcctca agcgagccgt gaaattgggt ctcagcaaac agtgcaacat gagcgatgcc
     1681 gcgctgcaca cattggcgca gtacaactgg cccggcaaca ttcgcgaatt ggagaatgtg
     1741 ctgacccgcg cctatattct gtcgccagga aagacgatcg aacctgagca cttatgtctc
     1801 tcagaatcgg tcattgcctg cgacccaatc gaggcacagc cccttcaaca ccggccgatg
     1861 gccggaaacg gctatcatga aatgatggaa gcctacagcc gttgggtgct cgaggaagcg
     1921 ttgaagaaaa acgactggaa tcagaccagg gcagccgaag cgctgggcct tcagcgaacc
     1981 tacttcacga agctgcttcg tcaaaaacag attcccgggc ggcagcccaa acctgcatcg
     2041 ccgtcgtcgt catcacaagc tgacatgaac cattgagaga cgccggcgct cctccgtgga
     2101 cagagagatc ctacctcggg taaaattgtg ctcctcgcat gccagctcct tcgacgtggc
     2161 ggtgtagctc agttggcaga gcagcggact cataagccgc gggtcacccg ttcgatccgg
     2221 gtcaccgcca ccaaaaaccg tcagcggttc cgcaacttat gagtcggctg ttcattggcc
     2281 atcttccttc acattcattt ttccggttcc aggtccggtt ggaatagcgg cgacgatttg
     2341 ccctgttgcg acaccacggt caacttgacc ttacccacct ggatatagaa taccgcctcg
     2401 gctgcctccc cttgcgaaaa cagcgtctgt ttctttaagg gcaggagatt ggttttgcct
     2461 tgccgctttg gtcagaaaca gctgagggtt gaagaggggc gttcgtgagc gtgccgtcgg
     2521 tcagcctttt ctaaattgga gtgtttccgt ttaagcatta cgagatggca gctgcacttt
     2581 cggtatcaag aagcgcatga ggcgagcgac cgacaatctc tcatctcgtg tggccgcgtt
     2641 ctacgtacta actgacagcg cgacaacagg acaagaagtc gaggaaaact atggtaggtg
     2701 tgaatgaccg atcccgaatg gtcaaaatcg ctcacattag aaagaccgtt ttgcagctcg
     2761 aaaagactcc ctcttgatcc gccattcctt catataaaag agcgcaactg caggttttct
     2821 ttccagaact cgctggcctc cagaccggaa tgggccaagt ctgccca
//
LOCUS       DASTQY010000219         6908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1219887, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000219 DASTQY010000000
VERSION     DASTQY010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219887"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..636
                     /locus_tag="VFR82_07190"
     CDS             268..636
                     /locus_tag="VFR82_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501420.1"
                     /translation="MKLLFGTVAMVSGVLFAVSMASANPALLPKHEGYPMKNSGSPVN
                     GQPTANDPGQSDARGASTLTKAAGFDDNHVSQNLKKAENDRVTASEGAGRLPKVQGPQ
                     IQIAPPVNSATKITGDRKIE"
     gene            complement(709..1251)
                     /locus_tag="VFR82_07195"
     CDS             complement(709..1251)
                     /locus_tag="VFR82_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEU4501421.1"
                     /translation="MRNTSPGKILTRAYFNRSTLVVARSLLGKYVVRENGKGMFAGKI
                     VEVEAYVGPHDLACHASKGRTLRTDVLFGPPGISYVYLIYGMYHCLNVVTEEAEFPAA
                     VLIRAAEINGSLIDGPGRLCRALDIDRSLNRLDMTAGRHLWFEDRGEKIHRGQVGTFP
                     RIGVAYAGVWAEKLYRFRIT"
     gene            complement(1382..3493)
                     /locus_tag="VFR82_07200"
     CDS             complement(1382..3493)
                     /locus_tag="VFR82_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4501422.1"
                     /translation="MNEMPEDRRGRLIAFPGFWTGCDVTYRSLMIVGFSVLLSAGRAL
                     GHDPDAEVLQVPEVSVTGERPVAASSQQFIPDKEYLLQPQGRPAQVLRLIPGFVAVEH
                     SGGAGKADQYFLRGFDADHGTDVAFFSDGMPINFRSHAHGQGYADLNFIIPETIEGLD
                     VYKGAYLPEYGDFATAGAANFRTRQVVKEGMLQAAGGEFDTQRYVLMFSPTKDKVRTL
                     FAGEGYYTNGAFLNDNRYFRANVLGKMTTNFSSRDELSLTATFHKAQWNASGEIPLRA
                     VADGTLDRFGAIDPSEGGKTLRSTARLNYHYDTRSGGQFFANAYAQYYKFDLYTNFTF
                     FLNDSINGDGFQQHDNRVMYGGDLGYTHRAELFGMPSIGTIGFQTRVDDIHARLATQV
                     RRNPLGTTTDSDILEASYAPYLRAEVQPLSWVRLAGGLRAESFTFDVKNRCADCAEQP
                     TGQTTSSIVLPKANVILGPWFHTEFFANYGEGYHSNDARSVVVPGSSPLARARTYEVG
                     LRSKPWGPEGIEFITTLWAIDLKSELVFAGDEGTTEIRGATRRRGIEAAVRGQVWGPI
                     YVNGSITWSKAEFVNGAAIPLAPELTAYGAILVQWPEGLRSQLQATYLGVRPLIEDRS
                     AKAPSWIDLDLSERYILPVKLPHGRLEAFLFIQNLLNTKWEQATFYFASRLRNEAAGI
                     NDIHFVPGNPRFFMGGLAWYF"
     gene            complement(3699..4121)
                     /gene="nikR"
                     /locus_tag="VFR82_07205"
     CDS             complement(3699..4121)
                     /gene="nikR"
                     /locus_tag="VFR82_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HEU4501423.1"
                     /translation="MKKLVRFGVSLDHHLLDEFDDVVRRRKYATRSEALRDLIRDQLV
                     NQEWSGNTNREVVATITFVYDHHVRDLTRKLTHIQHDFQGHVMAGMHVHLDHDHCLEV
                     LVVKGKVAEIREVADALVSVKGVKHGKLTMTTTGKGLV"
     gene            complement(4212..5624)
                     /locus_tag="VFR82_07210"
     CDS             complement(4212..5624)
                     /locus_tag="VFR82_07210"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEU4501424.1"
                     /translation="MAASVSFRVRVFAAAVLTLAAPTWGEAAGRLFPVASSTDLQNQV
                     KATASKVIPAVVSIASTVMVRDQAFSDEALPFGLFKEPPARRQYGQGSGVIVSSDGYI
                     ITNNHVVADAVDVEVILADRRQFKGKVVSTDPKTDVAVVKIQTTNLPTVPWGDSSNLA
                     VGDFVLAIGNPLGLSRTVTFGIVSAVGRADVGVADFEDFIQTDAPINPGNSGGALVNT
                     SGELVGINTAIASPTGGSVGVGFAIPSNMARAAMQSLIKTGRVVRGFLGASTQDVTPL
                     LGKIFRLPDVKGAIVTDLQAKGSAEKAGLKRGDVIERFDGRDVMDSGHLRNLIAAAAI
                     GSKHRLDLVREGKMIQVDLTVQEAPRERAKKAQAAATTASGTHPLSGVVFDDVTPPLA
                     RQMDLPVSSGVVVTDIEEGSSAESSGLQPGDVILELNRQPIPNFSTFQRLADPMKSSD
                     LALLLVNRQGTILYIPVRGE"
     gene            complement(5702..>6908)
                     /locus_tag="VFR82_07215"
     CDS             complement(5702..>6908)
                     /locus_tag="VFR82_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU4501425.1"
                     /translation="GYFVPVVVAISIVTAVAWAMWGPEPKLAFALVNAVAVLIIACPC
                     ALGLATPMSIMVGTGRGATAGVLVRKAETLEVLGKVDTLVFDKTGTLTEGKPKLVSIR
                     FVPPWNDVEILRLSATLERQSEHPVASAVIAGAEERQLTPGLVEQFESITGHGVMGVV
                     DRHRVAVGTTQFLRERLKVTDPGLAVLEEEAARLRAEGQTTVFVAVDDKAAGLLGVAD
                     PIKPSTAEAVRVLKEQGVRLVMVTGDHQLTADSVARRLGISEVHAGVLPDHKGRIIAG
                     LKSQGRVVAMAGDGINDAPALALADVGIAMGTGTDIAMESAGMTLVKGDLRALVRAVR
                     LSRATMANIRQNLFFAFVYNMVGVPIAAGVLYPFWGLLLNPMIASAAMTFSSVSVIAN
                     ALRLRHVDL"
BASE COUNT         1523 a         2045 c         1869 g         1471 t
ORIGIN      
        1 ctggcaacat cagatgtgca attctcccaa gcgcacaccg cccttatgta acaatatgat
       61 agatcctgcc tgcgcgcgcg gtgaacacag ccttctcacc cgctcactct cctggtgccg
      121 agacgccgtt cttcccagcc ggaaccggcc tctttgtgct tgacaggcct cattccctct
      181 ggctatcatc ccgcccactt gattcgctgt cgatatcgga acgcaactac ggcattcttc
      241 agccaccatt attcaaagga gattggtatg aagctcttat tcggcactgt tgcgatggtt
      301 tccggtgtct tgtttgcggt ttccatggcc tcggccaacc cagcgctgct gcccaagcat
      361 gagggctatc caatgaagaa cagcggcagc ccggtgaacg gacagccgac ggcgaacgat
      421 ccgggccagt ccgacgccag aggcgcatca acgttgacga aagccgcggg cttcgacgac
      481 aaccatgtgt ctcagaattt gaaaaaagct gagaacgacc gggttacggc gagcgagggc
      541 gctggccggc ttccaaaagt acaaggtccg cagatccaga ttgccccacc cgtcaactct
      601 gcgacgaaga ttacgggaga tcgcaagatc gagtaatcgc ttctgccgag catgaaaaag
      661 ggagtagccg tttgctggct gctccctttt ttcttttcaa ccctcttctc aggtgattct
      721 gaaccgatag agcttctcgg cccaaactcc tgcataggcg acgccgattc gtgggaacgt
      781 gcccacttga cctcgatgaa tcttctcgcc tcgatcttca aaccagagat gccgacccgc
      841 ggtcatgtct aatcggttca acgatcgatc gatgtccaat gcacggcaca accggccagg
      901 accgtcgatc agcgatccgt tgatttcggc tgccctgatc aacactgcgg caggaaattc
      961 agcttcctcc gtcacgacat tcaagcaatg gtacatgccg tagattagat acacatatga
     1021 gattcccggc ggtccgaaaa ggacatcagt cctcagggtt cggcccttcg atgcgtgaca
     1081 tgccaaatca tgcggcccga cgtatgcctc gacctctaca atcttgcccg caaacatccc
     1141 cttgccgttt tctcgcacca cgtactttcc taacagcgac cgggcaacga cgagtgtcga
     1201 ccgattgaaa taggcgcgcg tgagaatctt tcctggcgat gtatttctca agagtactct
     1261 ttaatggggc tgctcaaaac gacgtccaac aagcccgcag tgtcatagga ggcgtacttt
     1321 accacgtacg tggagggcct gagtaaccga gaacgcagtt ggaattgttt tcagccgccg
     1381 attagaagta ccaggccagg ccgcccatga agaatcgagg gttgccaggc acaaagtgaa
     1441 tatcgttgat cccggctgct tcattccgta accgtgatgc aaaataaaac gtagcctgtt
     1501 cccatttagt gttgaggaga ttttgaataa agaggaaggc ctccagccgt ccatgcggaa
     1561 gcttgacagg caggatatat cgttcagata gatcaaggtc gatccaggac ggcgccttgg
     1621 cgctccgatc ctcaatcaac gggcggacgc caagataagt cgcttggagt tgtgagcgca
     1681 agccttccgg ccattgaacg agaatggccc cataggcggt gagttcaggc gccagcggaa
     1741 tcgcggcccc attcacgaat tccgctttgg accatgtgat gctcccgttg acgtagatcg
     1801 gtccccatac ctggccacgc actgcggctt ctataccgcg gcgccgagtg gcgccacgaa
     1861 tttcagtcgt cccttcatca ccggcgaaca ccaattccga tttgagatcg atcgcccaca
     1921 aggtcgtgat gaattcgata ccctctggtc cccagggttt tgagcgcaac ccgacctcat
     1981 aggtgcgtgc acgagcgagt ggagaggaac cgggcaccac caccgagcgt gcatcgttgc
     2041 tatggtatcc ctcgccatag ttggcaaaga actccgtatg gaaccagggt cccaggatca
     2101 cattggcctt cggaagcacg atgcttgagg tcgtctgtcc agtcggctgt tccgcacaat
     2161 cagcgcaacg gtttttgaca tcgaacgtga aagattctgc acgaagccca cccgccaatc
     2221 gtacccacga cagaggttgg acttcagctc tgagataggg tgcgtaggat gcctccagga
     2281 tatcgctatc ggtcgtcgtg ccaagcggat tgcgtctcac ctgtgtagcc agccgcgcat
     2341 ggatatcatc caccctcgtt tggaaaccga tcgtgccaat actgggcatc ccgaagagct
     2401 ccgcccgatg tgtgtaaccg agatctccgc catacatcac tcgattatcg tgttgctgaa
     2461 agccgtcgcc gttgatcgag tcgttgagga agaaagtgaa attggtatac aggtcgaact
     2521 tataatactg ggcgtaggcg ttcgcgaaaa attgacctcc ggaccgtgta tcgtagtgat
     2581 agttcagccg agccgtactg cggagcgtct tgccgccttc cgacggatcg atggcgccga
     2641 agcgatcgag agtgccgtcc gcgaccgccc gcaacgggat ttctcccgac gcgttccatt
     2701 gagctttatg aaaggtcgcg gtcaggctta attcgtcacg gctggagaaa ttcgtagtca
     2761 tctttccaag aacattcgct cgaaaataac gattgtcgtt caaaaaagcg ccgttggtgt
     2821 agtacccctc tccggcgaac agggtgcgca ctttgtcttt tgtgggtgaa aacatgagaa
     2881 catagcgttg cgtatcgaac tctcctcctg ccgcctgtag cattccctct ttgacaacct
     2941 gccgtgttcg gaagttcgcc gccccggcgg tagcgaaatc gccatactcc gggagatagg
     3001 cgcctttgta cacatcaagt ccttcgatcg tttctggaat gataaagttc aggtcggcat
     3061 acccctgtcc atgcgcatga gagcgaaagt tgatcggcat gccgtccgaa aaaaacgcaa
     3121 catcggtgcc atgatcggca tcgaagccgc gcaggaaata ttggtcggct tttcccgcac
     3181 caccggaatg ttccactgcg acaaaaccgg ggattaggcg taggacctga gccggtcggc
     3241 cttgcggctg aagcagatat tctttatccg gaatgaattg ttgagacgag gccgcaacgg
     3301 gccgttcccc cgtgaccgat acctcgggga cttggaggac ctccgcatcc gggtcatggc
     3361 ccagcgcccg gcctgcgctc agcaggacac tgaaacccac aatcatcagc gagcggtatg
     3421 tcacgtcgca ccccgtccag aagcctggga acgcgatgag acgtccacga cggtcttccg
     3481 gcatctcatt catcgagccg ggccgacctc gtgacctggc gacaattgac tggagctgtt
     3541 gaaggtaact cacctaagcc gggatcagac agcgcgctac acccgacggc cttcagaaac
     3601 tttatcgagt ggcaggagct aatcctggcc ttctcggaaa catgtccggg agtccccgtc
     3661 gagcatcatc gctggcaatg ggcaatcacg gggcgcaatc acacgagtcc tttgccggtc
     3721 gtggtcatgg tcagcttgcc gtgtttgacc ccctttacgc taaccaacgc atcggcgacc
     3781 tctctgatct ctgcaacctt tcccttgacg accaacactt caagacagtg atcatgatcg
     3841 agatgaacgt gcatgccggc catgacatgc ccctgaaagt cgtgctggat atgggtgagt
     3901 ttcctggtga gatcgcgtac atgatggtcg tagacgaatg tgatcgtggc gaccacctca
     3961 cgattcgtat tgccgctcca ttcctgattc accaactgat cgcgaataag atcgcgcagc
     4021 gcctctgacc gcgtggcata tttccggcgc cggacgacgt cgtcaaactc atcgagcaga
     4081 tggtgatcta aagagacgcc aaaccggacg agcttcttca tgctccgatg ctacgattat
     4141 agaattcgtg ctacgcagta tagcggcacg aaagaagcgc gtcaattttc gagagtgcag
     4201 atgaggacgt gctattcccc tcgaacgggg atgtagagaa tggtcccttg ccggttgacg
     4261 agaagcagtg cgagatcgct tgatttcatg ggatcggcga gtcgctgaaa tgtggaaaaa
     4321 ttcggaatcg gctgacggtt taattccaaa atgacgtccc caggctgcag accggatgat
     4381 tccgcggaac tgccctcttc gatatcggtc acgaccaccc cgctgctgac cggtaaatcc
     4441 atttgccggg ccaggggagg agtgacatcg tcgaatacga cgccggacaa ggggtgggtg
     4501 cccgacgcgg tcgttgccgc cgcttgcgcc ttcttcgctc gctcgcgtgg cgcttcctgc
     4561 acggtgagat cgacttgtat cattttgcct tctcgtacga gatcaagccg gtgcttgctg
     4621 ccgatggccg ccgcggcgat caaattgcgg agatgcccgc tgtccatgac gtcccgcccg
     4681 tcgaaccgct caatgacgtc tcctcgcttc agcccggctt tctccgcgga cccctttgcc
     4741 tgtaaatccg tgacaattgc tcctttcacg tcaggcagcc gaaagatttt tcccaatagc
     4801 ggggtgacgt cctgtgtgga agcgcccaaa aatccacgta cgacgcgccc cgttttgatc
     4861 aggctctgca tggcggctcg agccatatta ctcgggatgg cgaagcccac ccccacgctg
     4921 ccccccgtgg ggctggcgat ggccgtattg atgccaacca gctcgccgct cgtgtttacg
     4981 agcgcgccac cggaatttcc cggattgatc ggcgcatccg tttgaataaa gtcctcgaag
     5041 tccgccacac cgacatctgc gcgcccgact gcgctgacaa tgccgaatgt caccgtgcgg
     5101 ctcaatccca acggattgcc gattgccaat acgaagtctc cgacggccaa attgctggaa
     5161 tctccccacg gaacagtagg caaattcgtg gtttggattt tgacgacggc gacgtcggtt
     5221 ttagggtcag tcgaaacgac cttgcctttg aattgtcgcc ggtcggctag aattacttcc
     5281 acatccacgg catcggccac cacgtgattg ttggtgatga tgtagccatc cgaagagacg
     5341 atgactcctg aaccttgccc atactgtcgt cgtgctggag gttccttgaa caatccgaag
     5401 ggaagcgctt catcgctgaa tgcctgatca cgcaccatga ccgtggaggc gatgctcacg
     5461 acggcaggaa tgactttgga tgcggtggcc ttgacctgat tctggagatc ggtcgagcta
     5521 gcgaccggaa acaggcgacc cgcggcctct ccccaagtcg gtgcggccag tgtgaggacg
     5581 gcggcagcga atactcgcac acggaaagaa accgaagctg ccataccagc ctttctcatg
     5641 gacatatgat gtgagcggtt caagaatgcc gtagcctagc atggaggcgg agaggacgca
     5701 ctcataaatc cacatgacgc agacggagcg cgttggcaat cacggaaacg gaactgaagg
     5761 tcatcgcggc gctcgcaatc atgggattaa gcagcagacc ccagaacgga tacagcactc
     5821 ccgccgcaat gggcacaccg accatattgt agacgaatgc aaaaaagagg ttttgtcgga
     5881 tgtttgccat ggtggctcgg ctcaagcgga cggcgcggac gagcgcccgg agatcgccct
     5941 ttacgagcgt catccctgca ctctccatcg cgatatctgt ccccgttccc attgcgattc
     6001 ccacatcagc gagcgcaagg gcgggcgcat cgttgatacc atcgccggcc atcgccacca
     6061 cccggccctg cgatttgagt cctgcgataa tccgaccttt gtgatccggc aatacaccgg
     6121 catggacttc tgaaattccc agccgtcgcg cgacagaatc tgccgtgagc tggtgatcac
     6181 cggtgaccat caccaagcgt acgccctgtt ccttcaagac tcggaccgct tccgcagtcg
     6241 acggctttat cggatcggcg acgcccaaca agccggcggc cttgtcgtcg acggcgacaa
     6301 agacggtcgt ctgaccctct gctcttaagc gtgcggcctc ttcttctagg accgctaagc
     6361 cgggatccgt caccttgaga cgctctctta aaaattgtgt ggtacccacg gccacccgat
     6421 gccggtcaac cactcccatc accccatgcc ctgtgatcga ctcaaattgc tcgaccaagc
     6481 ccggggtgag ttgtcgttct tcggcgccgg ctatgaccgc tgacgcaacc ggatgttcgc
     6541 tctggcgctc aagagtggcc gacagccgca gaatctcaac atcgttccag ggaggtacga
     6601 agcggatgga aactagttta ggtttgcctt cggtgagggt gccggttttg tcaaagacga
     6661 gggtatccac ttttcccagc acctcgagcg tttctgcttt gcggaccaag accccggccg
     6721 ttgccccgcg tcccgttccg accatgatcg acatgggtgt cgccaggccc agggcacaag
     6781 ggcacgcaat gatcaacact gccacggcgt tcaccaacgc aaacgccaac ttcggttcag
     6841 ggccccacat tgcccacgcc acagccgtga cgatcgatat ggcgacgaca accggaacaa
     6901 aatatcct
//
LOCUS       DASTQY010000220         4439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_2060, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000220 DASTQY010000000
VERSION     DASTQY010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2060"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /gene="glgX"
                     /locus_tag="VFR82_07220"
     CDS             complement(<1..1471)
                     /gene="glgX"
                     /locus_tag="VFR82_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEU4501426.1"
                     /translation="MPRVTTRPGHPYPLGATWDGEGVNFALFSENATGVELCLFDRPD
                     QEKETHSIRIEERTNQVWHVFLPEARPGQLYGYRVNGPYEPQAGHRFNANKLLIDPYA
                     KALHGSLRWSDSMFGYSIGDADADLSFDERNNASLVPKSVVIEQAFTWGDDKPLRRAW
                     SETVIYEMHVRGFTVKHPLIHEALRGTYAGLATRPVIEYLQRLGVTAIELLPVHAFVA
                     DKHLIDKGLTNYWGYNTIGFFAPELRYAAHPEHAVNQFKTMVKTLHSAGIEVILDVVY
                     NHTAEGNHLGPTLSFRGIDNAAYYRLVPDQPRYYVDYTGTGNTLNVQHQRTLQLIMDS
                     LRYWALEMHVDGFRFDLASALARELHDVDRLGGFLDIIHQDPVLSQLKLIAEPWDIGE
                     GGYQVGNFPAGWAEWNGKYRDTIRRYWKGDGGQASELAYRLSGSSDLYEGSGRRPFAS
                     INFVVAHDGFTLRDLVSYDQKHNEANKEDNKDGSDDNQSW"
     gene            complement(1539..2072)
                     /locus_tag="VFR82_07225"
     CDS             complement(1539..2072)
                     /locus_tag="VFR82_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU4501427.1"
                     /translation="MQEQQFVLDVKTIRDRARRHIENGAMTPGYKAKAPVVLRLLNDA
                     LATEIVCTLRYKRHYFMAKGLSSESVKSEFLEHAEEEQAHADAIAERIVQLGGEPNLS
                     PEGLLDRSHSQYAEGTSLEDMIKEDLVAERIAIDSYRQMIEYIGGDDPTTRRLLEEVT
                     AKEEEHAEDLASLLAKS"
     gene            2491..3150
                     /locus_tag="VFR82_07230"
     CDS             2491..3150
                     /locus_tag="VFR82_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4501428.1"
                     /translation="MSLHTHTAKVLLVDDHPLVLESIRQLLDSTFNIVGMVQDSGEII
                     SRALEHRPDVILLDACMPGLSGFAATKQLKKLLPSVKIILVTMLTEAISISEAFRAGA
                     SGYVLKQSASDELHAAIESVLANKRFLSKKIDPEVREAVEHEWFRPEGYSSDLTGRQR
                     EILVLLAEGRSTRHIAQQLNISMKTVEFHKANITRKLGVHTTSDLIKFALAQGITTLQ
                     F"
     gene            complement(3196..3861)
                     /locus_tag="VFR82_07235"
     CDS             complement(3196..3861)
                     /locus_tag="VFR82_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4501429.1"
                     /translation="MSDTYRILLADDHVLVAEGIQKLLEPEYELVGIVADGRSLVTAA
                     AKLQPDIVVVDISLPLLNGLDASQQLKKNNPNLKIIVLTMHSEPNFVTQAFRVGVSGY
                     VLKQSVGSELVQAIREVVKGRTFVSPMVAQSLVDQAVNPSPTGAPGEVKVGFAQTLSA
                     RQREVLQLVAEGKATKEIASILNVSVKTVEFHKTRIMKELRLRTAAELTKYAIAAGLT
                     SIH"
BASE COUNT          978 a         1186 c         1207 g         1068 t
ORIGIN      
        1 tccaactctg gttatcgtcc gatccgtcct tgttatcttc cttattcgct tcattgtgct
       61 tctgatcgta cgagacgagg tcacgcaagg tgaagccatc atgggcgacg acaaagttga
      121 tgctcgcgaa tggacggcgt ccgctgcctt catagaggtc gctgctgccc gacaggcgat
      181 aggccaattc gctggcttgg ccgccgtcgc ctttccaata gcgtcggatg gtgtcccggt
      241 atttgccatt ccattccgcc cagccggcgg gaaagtttcc cacctgatat ccgccttcgc
      301 caatgtccca cggttcagcg atcagcttaa gctgtgaaag gacggggtct tggtgaatga
      361 tgtccaggaa gccacccagg cggtcgacat cgtgtagttc acgggcgaga gcggaagcca
      421 gatcgaatcg aaatccgtcg acgtgcatct cgagcgccca gtaccgcaag ctgtccataa
      481 tgagctgaag tgtgcgttga tgttgcacgt tgagcgtgtt gccggtgccc gtgtaatcga
      541 cgtagtagcg gggctgatcc ggcacgagac ggtagtaggc ggcattgtcg ataccacgaa
      601 aagacaaggt agggcccagg tggttgcctt cagcggtgtg gttatagacg acatcgagaa
      661 tcacctcgat gcccgcgctg tgcaacgtct tgaccatggt tttgaattgg ttcacggcat
      721 gttccggatg ggctgcatag cgcaactccg gcgcaaagaa gccgatggtg ttgtagcccc
      781 aatagttggt caagcctttg tcaatcagat gcttatccgc gacaaaagca tgcacaggga
      841 gcagttctat ggcggtcacg ccgagccgtt ggaggtattc gatgactgga cgcgtcgcca
      901 aaccggcata ggtcccgcgg agtgcctcat ggatcagcgg gtgtttgaca gtgaatcccc
      961 ggacatgcat ttcataaatg accgtctcgc tccatgcacg tcgcagaggc ttatcgtctc
     1021 cccacgtgaa cgcctgctcg atcaccactg acttcggcac cagcgaggca ttgttccgtt
     1081 cgtcgaagga gaggtcggcg tccgcgtctc cgatgctgta gccaaacatc gaatcagacc
     1141 atcgtagtga cccatgcaag gctttggcat agggatcgat caacaacttg ttcgcgttga
     1201 agcggtggcc ggcttgcggt tcataaggac cgttgacgcg atagccgtaa agttgccctg
     1261 gccgcgcttc agggaggaac acatgccata cttgattcgt ccgctcctcg atcctgatag
     1321 aatgggtttc tttttcctga tccgggcggt caaacagaca gagttccact cccgtggcat
     1381 tttccgagaa cagggcgaaa ttgacacctt caccatccca tgtcgcgcct aacggatagg
     1441 gatgaccggg acgagtagtg acacgcggca tagcgcactc cttctctaac aatttactgc
     1501 gacaaataac tcacgagggc ccacactctc tgatcgactc acgattttgc aagtagactg
     1561 gcgagatcct ccgcatgctc ctcttctttg gcagtcactt cttcaaggag ccggcgcgta
     1621 gttggatcgt ctccgccgat atattcgatc atctggcgat aactgtcgat agcaatccgt
     1681 tcggcaacca gatcttcctt aatcatgtcc tccaacgatg tcccctcggc atattgcgag
     1741 tgactacgat ccagcaggcc ctcgggggac aaattcggct cgccgcctaa ctgtacgatg
     1801 cgctcggcga tggcatccgc atgggcttgt tcttcttcgg catgttcgag aaattcgctc
     1861 ttgacacttt ctgaactgag accttttgcc ataaaatagt ggcgtttgta tcgaagcgta
     1921 cagacaattt ccgtcgcgag cgcatcgttg agcagccgga gcacgacggg cgctttggct
     1981 ttatagcctg gcgtcatggc gccgttctcg atatgacggc gtgccctgtc acgaatggtt
     2041 ttgacatcga gcacgaattg ttgctcttgc attacatcct ccttgtggca tcggttacga
     2101 ctgtgttcgt tgatgagtgg gatggaattc gcgagaggag gggacgcaca gactgagcct
     2161 accttactct gcggcggtgc cgtagtgtgt attgtgccag gcggcaccgg ttcattccga
     2221 agcgacgaaa tccagtgcgg tggaaagcgt gataggggaa cgctccccgc cggatgtctt
     2281 accagatctt caaataacct aaagctgagt agggccttgc gcacactcgg agcaccccta
     2341 gggtttccct cttagcgcta ggggatggcc cagttcatgc ttttcctcag ttgaggtaaa
     2401 acaaatcaac ggtttattta cttgaaccga ggggctcatt tgtaccatga tagggggcgg
     2461 gggagtttcc gctaaaacga gacgaacaga atgtcgttac acacccatac cgccaaagtg
     2521 ctcttggtcg atgatcatcc cctcgttctc gagagtattc gccagcttct cgattctact
     2581 tttaatatag tcgggatggt tcaggattcc ggggaaatca tcagccgcgc gctggaacac
     2641 cgcccggatg tcattttatt ggacgcgtgc atgcccggac tcagcgggtt cgcggccacc
     2701 aagcagctga agaaactgct tccctcggtc aagatcatcc tggtgaccat gttgacggaa
     2761 gcgatatcga tcagcgaggc tttccgggcc ggcgccagtg ggtacgtctt gaagcagtcc
     2821 gcctccgatg agctgcatgc agccattgag agcgtgctcg cgaacaagcg ctttctctcg
     2881 aaaaagattg atccagaggt tcgggaagcg gttgagcacg aatggtttag accggaaggt
     2941 tattccagcg atttgaccgg tcggcaacga gaaattttgg tgctactggc agaagggcgg
     3001 tcgaccaggc atattgctca acagctgaat atttccatga agaccgtgga attccacaaa
     3061 gccaacatta cgagaaaact cggcgtccat accacttctg atctgatcaa gtttgctctg
     3121 gcccaaggga tcaccacact gcagttctaa accctatcct tccgacttca gctcgaattt
     3181 caactcaagc aacgttcagt gtattgaggt taagcctgcc gcgatggcat acttcgtcag
     3241 ctcagccgcc gtgcgcaggc gcaattcctt cataattcgg gttttgtgaa attctacggt
     3301 tttgacggac acgttgagga tggaagcaat ctctttggtc gccttgccct ctgccaccag
     3361 ctgaagcact tcccgttggc gagcgcttaa cgtttgtgca aatcctacct ttacttctcc
     3421 cggagccccg gttggagagg gatttacagc ctggtcaact agactttgcg cgaccatggg
     3481 agacacgaac gtgcgcccct tgaccacttc acggatcgcc tgcaccaact cagaaccgac
     3541 cgattgcttc aagacataac cggatactcc gacgcggaag gcttgcgtca cgaaattcgg
     3601 ctcagaatgc atggtgagca cgatgatctt cagattaggg ttatttttct tgagttgctg
     3661 ggacgcatcg aggccgttca agagcggcaa ggagatatcc accacaacga tgtccggctg
     3721 aagcttcgct gccgccgtga cgagtgagcg tccgtcggcg acgatgccga cgagctcgta
     3781 ttccggctcg agcagtttct gaatgccttc tgccaccagc acatggtcat cagccaggag
     3841 tatccgatat gtgtctgaca tagggtgtac cctcgaatat agtgtgggag acgctagtgt
     3901 cttgctggtg tatgaaagaa gtgtacctgg attgtaagtg ccctcacaag ggtatgcacg
     3961 aggggaggaa tctaggggtt ttccgagtgc ggcaactaac tcgatgtcgg cagaaacaat
     4021 aaaaaatctt cgcgttgcgg tattgcccta atagctaatc aatgcgctgg caagaacccc
     4081 tgcctttgtt gcaaatcgca ggcagtaggc cagtttcagt ccacagagct agggatgtga
     4141 ggacacgaat tgaatgttgg gctaatcgcc gctatcagtc atgccatcgt ccctgggaca
     4201 tctttatcca tggcaagctc ctggtcagtc ttcccaggcc gcgttggcgc tagatccagg
     4261 gctgttggct cttcatggaa tgttcacata atcgttttcg atgatcccat gctcgaagta
     4321 agaaattaca accagtgctc acaagagcat ttggaagttt tctttcacaa ctccgctcga
     4381 ttcgtacgct ccgcgacatc tttctgaaca cggcctcttt tcagtctcgg caagggtga
//
LOCUS       DASTQY010000221        10719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_557454, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000221 DASTQY010000000
VERSION     DASTQY010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10719
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557454"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFR82_07240"
     CDS             <1..286
                     /locus_tag="VFR82_07240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4501430.1"
                     /translation="DVESLRQHYALTLDAWGQRLERHHQDALRAVGEVTYRVWRVYMA
                     MAAYQFRLGRLSLHHTVCVKAVDGQTELPLTRKDWYESRISEARVHQEAA"
     gene            396..1700
                     /locus_tag="VFR82_07245"
     CDS             396..1700
                     /locus_tag="VFR82_07245"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4501431.1"
                     /translation="MPKPATRGAKSAGWRQLLYDRSVRWLYAGQIISQVGDGVSKVAL
                     LWFVYAMTDSALKMSLIGVLQTIPPLLFGPFAGVLLDRIDKRRAMIVIDLVRTGLLSS
                     IPILYAMGLLSLPVLYVLVFVIAMFSMAFGPALNATLPLIVKKDQLTRINALMQSAMT
                     IGQLLGPALSGILIAAIGAQNALYVNAGAFLISALCKLPLRLPDIRPHRNSHNVWQQA
                     VKDLQDGIRFVFVKQRLLFLLMIVASIFTLGSTAFVYLLPVIGDRLLHVGSVALGWLW
                     SSLSVGILMTTAWLLWRPQQSICARIWMIAVAAGVAAFAVPALLLSESVLVAAGLIAG
                     IGATSGLITPLVSASLQERTPKDLMARVFGLFNTGTMVFSMIGMTLFGWLADSFGPGV
                     SLTTIGVVNGAAAIVSFVLLPWCYRLAKETSEQSAQSIRRAS"
     gene            1829..2458
                     /locus_tag="VFR82_07250"
     CDS             1829..2458
                     /locus_tag="VFR82_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4501432.1"
                     /translation="MRTRAVWCVLLAGMAWSFALSGHAQAEYYVAGQVGVNFADRLKN
                     IEGTGSLLNLRAPDFDLKNSVSYGGKLGYFPGNGPMGVELDVFNSQPNIKNLDDVPGI
                     HLGVTNVGVNLLMRYPGQTFQPYVGIGGALLIARLSESATTRSDTDVGFGLNVLAGVR
                     AFITPYVAIFTEYKYTHGTLTFDDAFGPVGGFSGDYQVQNLMFGVSYHF"
     gene            2471..3055
                     /locus_tag="VFR82_07255"
     CDS             2471..3055
                     /locus_tag="VFR82_07255"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HEU4501433.1"
                     /translation="MSTVMGLTFFRRLAACGLVCTATACSGPQVIPSELEQQIDSSVS
                     FRDIQASPQSMQGKTVLLGGEVLAARRVQDGTELEILQLPVTTDDPPAERRSESQGRF
                     LALDRTLTDPASLPPGTRLTLVGAVTGEETRRLDESTYRYPTVEAKHMYVWEPDAYLE
                     RSRRPVGLFGGVGFGFGSGGRSGSFGGVGIGTGF"
     gene            complement(3141..4502)
                     /locus_tag="VFR82_07260"
     CDS             complement(3141..4502)
                     /locus_tag="VFR82_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501434.1"
                     /translation="MRLFGRFFIFILLSTYAATLLTSCAGEDAGSSSALGTFTAAGGF
                     IEQVPGDLRPRWSAAELRTFLPTTRGKFLFPAPYNTEAFRITDASDCGGTDCVTPVGY
                     SYWRNSNAHAGSNDMWIFLSLNSQKGGSGPTLFRLDKASDTITKVGPLFPTTSKFSGR
                     SGDGWYFSASRANQLYINDGPKLLRYDVTTHLFETVFDITGQFGSDREVWQMHSSNDD
                     LVHSATLRAKGTGSFLGCLVFSEATGQFSFFPKKGTYDECHIDKSGHYLMILENTDGR
                     NGSENVIVDLQTGVETVIYDEKGGVGHADMGYNYVVGADGYNALPNAFITWSFAPSLL
                     KGPTVFHSSSSSIPRVNHVSHQNAKPGLPMHQQFACGSSADRGAAQNEILCFLLDGTE
                     RELVVAPVMTDLNASGGGSEYYKLPKGNLDITGQYFIWTTNMGGNRIDAFLVKVPAQL
                     LLS"
     gene            4946..5680
                     /locus_tag="VFR82_07265"
     CDS             4946..5680
                     /locus_tag="VFR82_07265"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HEU4501435.1"
                     /translation="MNRTQAIIALSVCIVMLGSSCAKSAHQTGEDQLQRLIPSAVLSQ
                     VDETVSFSDLLSEPGNYVGRTVMFSGLALKSRRAKDGTEIEVLQLPTDRGMSPSDRKA
                     RSEGRFLAVQTNGFLDPAVIEKDSPVTVVGEVKGATTKPLDEGEYQYPVLDIKQLVDW
                     NEVRTRERDTYYDSPYAGRYGGYYGPWSSWYYGFGSPFAGPYGFYPYSYYGPYYGPYY
                     GFPRGYSVPAPAPPPASSIPPQFQKD"
     gene            5687..8332
                     /gene="ligD"
                     /locus_tag="VFR82_07270"
     CDS             5687..8332
                     /gene="ligD"
                     /locus_tag="VFR82_07270"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEU4501436.1"
                     /translation="MKRATVASKLQRYRHKRNFVKTPEPAGHPGVIRQTAGPLYVVQK
                     HAATRLHYDFRLELDGTLKSWAVPKGPSLDPSQKRLAVHVEDHPLEYAKFEGVIPPKQ
                     YGAGTVLIWDRGTWQPQGNPQDGYRQGVLKFRLDGRKLHGAWTLVRMQGRRDARDNDK
                     ENWLLIKEQDADARKGAAGEIVESLTKSVESGRDLKDVATKDARVWQSNRSARASTAQ
                     AAAYSSRPPSSSRPVRSAQDFSSVAGAVKARQPDWIAPQLATLVDRMPTDEGWLHEIK
                     FDGYRLLCRVRNGEARLFTRNGNDWTAKLQNQADAIERLGMKEAWLDGEAVVFAEDGK
                     SSFQALQNAFDSRFTGTIVYCVFDLLYANGYDLRQTPLIERKRLLASLLNGRRGDSVL
                     RYSDHIIGDNQASFEEACRQGLEGLIVKRMDSTYKAGRGRSWIKVKCEQRQEFVIGGF
                     TEPAGSRAGFGALLIGYYENGQLYYAGKVGTGFTDNLLQKLYRMLGALEQPDPPFANP
                     PTGYEAKGAHWVTPKLVAEVRFAEWTQEGILRQASFQGLRTDKPAKAIGRERPQQSPG
                     ERRTVPVDKEQRSQETTGKRPLKGLRLTNPDRVLYPDIGLTKAGLVRYYEQIGDWMLP
                     HVKGRPLTLVRCPEGYQKCFYQKHVNEKVPPAVGRIEIEEENGRDTYMLAESLEALLG
                     LVQMGVLEIHTWGSLKDKVERPDRLIFDLDPDPSVPWRQVIEAAQLTKALLEELGFVT
                     FLKTTGGKGLHIVTPIQRTVDWEEAKGFAKMVADHLVSTIPQRFTSNMSKRARKEKIF
                     IDYLRNARGATAIAAFSTRAKPGAPVSVPIAWEELSETLPSDHFTVANLPVRLQHLRQ
                     DPWKTYDKSARRVTADMKARLKSGL"
     gene            8535..9668
                     /gene="tal"
                     /locus_tag="VFR82_07275"
     CDS             8535..9668
                     /gene="tal"
                     /locus_tag="VFR82_07275"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEU4501437.1"
                     /translation="MSRLLELQRCGQSYWLDNLTRGMMRSGDLKRRIDEEGLRGVTAN
                     PSTFALALRSGDYDADIERLARQDHSTEQIYEELLVRDVQEACDLFTGINHASDRLDG
                     YVSLEVSPHLAHDATGTMREARRFRELVDRPNLLVKVPGTTAGFLAIEQLLYEGISIN
                     VTLLFSLPAYEAVIEAYLSALERRSAEKRSLKDVTSVASFFLSRIDRLVDQLLLQLDR
                     PSEGRPMSAALRGRAAVASAKLAYRSFRRAFGGERWDRLAVKGATLQRPLWASTGTKD
                     PSYRDVMYVEPLIGRDTVNTMPEQTIRAFADHGQVKPDQIEQEVEAAARTIQGLSETG
                     IDLRCVAWQLEHEGIRQFTEAFDGAHREVEEKRRLAVSRPAAA"
     gene            9710..>10719
                     /locus_tag="VFR82_07280"
     CDS             9710..>10719
                     /locus_tag="VFR82_07280"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU4501438.1"
                     /translation="MKVAFNGMGRIGRATFKILATNPELDVVAINDVSPPDHLAYLLN
                     HDTVYGRYANRVTVRNGSLTMADRSYPVFAEPDPARLPWGDLEVDLVFECTGQFNQRH
                     DLSKHLDAGAKRVILSAPSKDNDIPTVVFGVNVYDEKNGAILSCGSCTTNCVAPIVEI
                     IERRLGLRKAVMNTVHAYTASQGIVDGSKENMRRGRAGAANLVPSSSGAATATARALP
                     SLQGRFEATAVRAPVPICSIADVVMLTARATSVEEVNALFLEEARSERYEGVVGVTED
                     PVVSTDIIQDPRASIIDREMTQVVDGDLVKVMSWYDNEWGYASQMVRQAVSMAQIVPV
                     EPA"
BASE COUNT         2263 a         2967 c         3234 g         2255 t
ORIGIN      
        1 cgatgtcgag agtttacggc aacactatgc cctcacgctc gacgcctggg gacagcgact
       61 agaacggcat catcaggacg ccctgcgcgc cgtgggcgag gtgacctatc gcgtctggcg
      121 ggtctatatg gccatggcgg cctatcagtt ccgcctaggg cgtctcagcc ttcaccatac
      181 ggtgtgtgtg aaagccgtgg acgggcagac ggagcttccg cttacccgga aggattggta
      241 cgagagtcgc atctccgaag ctcgagtgca ccaggaagct gcataagtgc ccgcaccaca
      301 cgtcgggcgg cgtccagagc tgcaaccgaa acaaccgtct tggcctgcgg tcggtccacg
      361 gtattgccaa acgcgaaagg cgatcacggg tttccatgcc taaacctgcc accagaggag
      421 ccaaatcggc cggatggcga caactgctct atgaccggag cgtgcgatgg ctctacgccg
      481 ggcaaatcat ctctcaagta ggcgacggcg tcagcaaggt ggcgttgctg tggttcgtgt
      541 atgccatgac cgattccgcg ctgaagatgt cgctgatcgg cgtgctccaa acgatcccgc
      601 ctttactctt cggtcccttt gccggcgtgc tgttggatcg catcgacaaa cggcgcgcca
      661 tgattgtgat cgatctcgta cgaacgggct tgctgtcgtc tattcccatt ttgtacgcca
      721 tgggcttgtt gtctttgcct gtactctatg tgctggtatt cgtgatcgcc atgttctcca
      781 tggccttcgg accggcgttg aacgccacgt tgccgctcat cgtaaaaaaa gaccagctca
      841 cgcgtatcaa tgcgttaatg cagagcgcca tgaccatcgg acagctcctc ggcccagcgc
      901 tcagcggcat attgatcgcg gcgatcggcg cccagaatgc gttgtatgtg aacgccgggg
      961 cattccttat ttcagcgctg tgcaagctgc cgctacgcct gcccgatatc cgtccacacc
     1021 gcaatagtca caatgtctgg cagcaagccg tgaaggacct ccaagatggc atcagattcg
     1081 tcttcgtcaa gcagcgcctg ctgtttctcc ttatgatcgt cgcgtcgatc tttacgttgg
     1141 ggtcgacggc gtttgtctat ctcttaccgg tcatcggcga tcggctgctg cacgtcggat
     1201 cggtcgcact cggatggctg tggtcatcgc tgagcgtggg aatcttaatg acgaccgcgt
     1261 ggttgctctg gagaccgcag cagtcgattt gcgcacgtat ctggatgatt gccgttgcgg
     1321 ctggtgtggc ggccttcgcc gtgccggcat tgctgttgag cgaatcggta ctggtggccg
     1381 ctggcctcat cgccggcatt ggggcgacgt cgggtctcat cacgccgctg gtgtccgctt
     1441 cgctacaaga aaggacgccg aaggatttga tggcacgggt gttcggcctg tttaacaccg
     1501 gcacgatggt gttttccatg atcggcatga ccctgttcgg ctggctcgcg gattcattcg
     1561 ggccgggtgt cagcctgact acgatcggcg tcgtgaacgg tgccgcagcc atcgtgtcgt
     1621 tcgtgctgct tccctggtgc tatcgcctcg cgaaggaaac gtcggagcaa tcggcgcaat
     1681 ccattcgacg cgcctcgtaa gtggacagtt tgtctccctc tcgagacggc atggcattgt
     1741 tcgaccgaga attcggggaa cgcgccctgt gtgtctgccc acccaatcca cttcgggtct
     1801 ggccctggaa ttgcaaaggt ttcaacacat gagaacgaga gcggtctggt gtgtcttgct
     1861 ggcggggatg gcctggtcat ttgcgctcag cggccatgca caagcggaat actatgtggc
     1921 aggacaagtc ggcgtgaatt ttgccgaccg gctcaaaaac atcgaaggaa ccggcagtct
     1981 cctgaatcta cgggcacccg acttcgatct gaagaattct gtctcctacg ggggaaagct
     2041 ggggtatttt cctgggaacg gtccgatggg tgttgagctc gatgtattca atagtcaacc
     2101 gaacatcaag aatctcgatg acgttcccgg aattcacctc ggcgtcacga acgtcggggt
     2161 gaatttgctc atgcggtatc ccgggcagac gtttcaaccg tatgtcggca ttggtggcgc
     2221 gttactgatt gcacggctat cggaaagtgc gacgaccaga agcgatacgg atgtcggatt
     2281 cgggttaaac gtccttgcgg gagtgcgggc attcatcacg ccctatgtgg cgatcttcac
     2341 cgagtacaaa tacactcatg gtaccctgac ttttgatgat gcctttggtc cggtaggagg
     2401 attcagcggc gattaccagg ttcagaacct catgttcggc gtctcatacc atttctaagc
     2461 gagaagggac atgtcaaccg tcatgggtct cacattcttc cgacgcctgg cggcttgcgg
     2521 gttggtttgc acggcgacgg cctgctcggg tcctcaggtg atcccctccg agctggagca
     2581 acaaatcgat tcgtccgtat cttttcgcga cattcaggct tctccgcaaa gcatgcaagg
     2641 caaaaccgta ctgctgggtg gagaagtgtt ggccgccagg cgtgtgcaag acgggaccga
     2701 actggaaatt ctccagctcc ccgtgaccac cgacgacccg cccgccgaac gacgaagcga
     2761 atcccaaggg aggtttctcg cgcttgaccg gaccctgacc gatccggcct ccttaccgcc
     2821 aggcacgcgc ctcacgcttg tcggagctgt gaccggcgaa gagacccgcc gccttgacga
     2881 gtccacttat cgatacccta ccgtcgaggc caagcacatg tacgtgtggg agccggacgc
     2941 ctatctcgaa agatcgcgcc ggccagtcgg actgttcggc ggggtgggat tcggctttgg
     3001 aagcggcggc agaagcggga gctttggggg agtcgggatc ggcacgggtt tctaatcact
     3061 ctgcactttg caacccgccc cgattgcgag cgtgatcagc tggtcagcat gcctgcagga
     3121 ctttcgcgca cctgaacgag tcaactgagc aggagttgag caggaacctt caccaagaac
     3181 gcgtcgatcc ggttgcctcc catattcgtc gtccagatga aatactgccc ggtgatgtcg
     3241 agattgccct tcggcaattt atagtactcg ctccccccgc cggacgcatt gagatcggtc
     3301 atgaccggcg ccaccacgag ctcccgctca gtcccgtcga gcaggaaaca caaaatttcg
     3361 ttttgtgccg cccctcgatc ggcgctgctg ccgcaggcga attgttggtg catcggcaac
     3421 cccggcttgg cgttttgatg ggagacgtga ttcacgcggg ggatactcga gctcgaacta
     3481 tgaaagacag tagggccctt gagcaaagac ggagcgaagc tccacgtgat gaaggcgttg
     3541 ggcagagcat tgtacccgtc ggctccgacc acataattgt agcccatatc cgcatgcccc
     3601 acgccaccct tctcatcata aatcacggtc tcgacaccgg tttgtaaatc cacgatgacg
     3661 ttctccgacc cgttgcggcc gtcggtattt tcgagaatca tgagatagtg gccgcttttg
     3721 tcgatatggc actcgtcgta ggtgcctttc ttcggaaaaa atgaaaactg ccccgtcgct
     3781 tcgctgaaga ccagacagcc caaaaatgaa ccagtcccct tggcgcgcaa ggtggcgctg
     3841 tggacgaggt cgtcgttgct ggagtgcatt tgccagacct cgcgatcgct tccgaattga
     3901 cccgtgatgt cgaagaccgt ttcaaagaga tgcgtggtga cgtcgtatcg caacagcttc
     3961 ggtccgtcat tgatgtacag ctggttggcc ctgctggcgc tgaaatacca cccatcacca
     4021 cttctgccgg aaaacttgct ggttgtggga aagagcggcc cgacttttgt aatggtgtcg
     4081 ctggccttgt ctaatcggaa cagtgtcggg ccagagccac ctttctggct gttgaggctc
     4141 aggaaaatcc acatgtcgtt gctgcctgcg tgcgcgttgc tgttccgcca gtaagaatag
     4201 ccgacagggg tcacgcagtc cgtgcctccg caatccgatg cgtcggtgat gcggaaggcc
     4261 tcggtattgt agggtgcggg aaagaggaat ttcccccggg tggtcggaag gaatgtgcgt
     4321 aactccgctg cactccagcg aggtcgaagg tctccaggta cctgttcgat gaaaccacct
     4381 gcagccgtaa atgtcccgag cgccgaagac gagcccgcat cctcgccagc gcaggaagta
     4441 aggagggtcg cggcgtaagt cgacaagagg ataaagatga agaaccggcc gaacaacctc
     4501 ataggcaacc tcgtcaggga ttggcgattg gattggcgtt cttgaatgcc gcgacgcgac
     4561 acgtactggc ggcgcaacca tatcgcgacc cttttcggca gttgcaaaga accggggcgt
     4621 gctatgtaaa aaatcagcga agagagtgtt gagaggcggt aaaaagatcg attctcagct
     4681 gatctaccta ggtggactca accactattc gtgcgacatg cccatcggtg gcatcactac
     4741 gcgtggtatg gaaggaaaat gcactcgtgc tccgtcgggc ttcttgcccg tctgaggatt
     4801 gactggtgag gagatggtcc taggcagacg catgggcatg acgacctgac ctggaggaaa
     4861 cgccagcttc attggtggtc accccttggt aaattcaatt ggggggtggt gtaataaagg
     4921 acaacgtcat gtatccatcc ggtttatgaa ccgaacacaa gcaatcattg ccctgtctgt
     4981 ctgcatcgtg atgctgggtt caagctgcgc aaaatccgcc catcaaacgg gagaggatca
     5041 gttgcagcgc ttgatcccat ccgccgtgtt atcccaagtg gatgagaccg tgtcgttctc
     5101 cgatcttctc tccgaacccg gtaactatgt cggccgcacg gtcatgttca gcgggttggc
     5161 tctcaaatcc cggcgggcaa aagacgggac ggagattgag gttctgcaac tcccaacgga
     5221 tcgtggcatg tcgccgtcgg accgaaaggc aagatcggag ggacgctttt tggcggtgca
     5281 gacgaacgga tttttagatc ccgccgtcat tgagaaggac tcgccggtga ccgtggtggg
     5341 agaggtcaag ggtgcgacga ccaaaccgct tgacgaaggc gagtatcagt atccggtcct
     5401 cgatatcaag cagctggtcg actggaacga ggtgagaact cgggagcgcg acacatacta
     5461 tgactcgccg tacgctggcc gttatggcgg atactacggg ccctggtcat cgtggtatta
     5521 cggcttcgga agtcctttcg ccggccccta cggattctat ccctattctt actatggacc
     5581 ctattacggt ccatattatg ggttcccccg cggctattcg gttccggctc ccgctcctcc
     5641 tccggcttcg tccattccac cccaattcca aaaagactag gggcctatga agcgcgcgac
     5701 ggttgccagc aagctccaac gctatcgcca taaacgcaat ttcgtgaaga cgccggaacc
     5761 cgctggacat cccggcgtga tccgtcagac cgccggtccc ctatatgtgg tgcagaaaca
     5821 tgccgccaca cgtctgcact atgattttcg gctggaactc gacggcacac tgaagagttg
     5881 ggctgttccc aaaggcccca gcctcgatcc ttctcagaag cggctggcgg ttcatgtcga
     5941 agatcatccg ctggaatatg ccaagttcga aggcgtgatt ccaccaaaac aatacggtgc
     6001 agggacggtg ctgatctggg atcgggggac ctggcaaccg cagggcaatc cgcaagacgg
     6061 ctatcgccag ggagtgctta agtttcgatt ggacggccga aagctccacg gcgcctggac
     6121 acttgtgcgc atgcagggtc gacgggatgc gcgcgacaat gacaaggaga actggctgct
     6181 gatcaaagaa caggatgcgg acgctcgaaa aggcgcagcc ggtgaaattg tggagagctt
     6241 gacgaaaagt gtcgagagcg ggcgagatct gaaagacgtc gccacgaagg atgctcgcgt
     6301 ttggcagtct aaccgaagtg cacgtgcttc caccgctcaa gcggccgctt acagctcccg
     6361 ccctccatcc tcttcgcgtc cggtgcggag cgctcaagac ttttcgtcag tggccggagc
     6421 cgtgaaggca cgccaaccgg attggatcgc gccgcaactg gcgacgttgg ttgaccgcat
     6481 gccgacggat gaggggtggt tgcatgaaat taaatttgac gggtaccgcc tgctctgtcg
     6541 agtgagaaac ggcgaggcgc gcctgttcac caggaatggc aatgattgga cagctaagct
     6601 gcagaatcag gccgatgcca ttgaacgttt ggggatgaag gaagcctggc tcgacggcga
     6661 ggcagtcgtc tttgccgagg acggaaaatc cagcttccag gccttgcaga atgccttcga
     6721 ttcccgattc accggcacca ttgtgtactg cgtgttcgac ctgttgtatg cgaacggata
     6781 tgacttacgc cagactccac ttattgagcg gaaacgtctc ctggcttccc tcttgaacgg
     6841 tcgacggggg gattccgtgc tcaggtatag cgatcatatt atcggagaca accaggcctc
     6901 gtttgaggaa gcctgtcggc aggggcttga aggtttgatc gtcaaacgaa tggattccac
     6961 atacaaggcg ggccggggcc gcagctggat caaagtgaag tgcgaacagc ggcaggagtt
     7021 cgtaatcgga ggttttactg agcccgcggg atcacgggca ggattcggcg cgttgctcat
     7081 cggctactac gagaacggcc agttatacta tgccggcaag gtcggcacag gttttactga
     7141 caacctgctg cagaagctct atcgcatgct gggtgcgctt gaacagcctg atccaccgtt
     7201 cgccaatccg ccgacggggt acgaggcaaa gggcgctcac tgggtcactc cgaagctggt
     7261 cgctgaggtc cggttcgcag aatggactca ggagggcatt ctgcggcagg cgagtttcca
     7321 gggtctcagg acggacaagc cggccaaagc catcggtcgg gaacgcccgc agcaatctcc
     7381 gggggagagg aggactgtgc cagtcgacaa ggagcagagg agtcaggaga caaccggcaa
     7441 gcggcccctc aagggactac gactgacgaa tcccgatcgg gtcttgtatc cggacatcgg
     7501 tctcaccaaa gcaggactcg tccggtacta cgaacagatc ggggactgga tgctccccca
     7561 cgtgaagggt cggccgctga cgctggtccg ttgcccggag ggataccaaa aatgctttta
     7621 ccaaaagcat gtgaatgaaa aggtgcctcc ggccgtcggt cgcatcgaga tcgaagagga
     7681 gaacggccgc gacacctaca tgctggccga gtcgctggag gcgctgttgg gccttgtgca
     7741 gatgggcgtc ttggagatcc atacttgggg ttccctcaag gataaggtgg aacgtcccga
     7801 tcgcctgatc ttcgatcttg acccggaccc ttcggtacct tggagacaag tgattgaagc
     7861 agcccagttg accaaagcac tgctggagga actcggtttc gtcacgttcc tcaagacgac
     7921 gggtggcaag ggtctgcata tcgtcacccc catccagcgt acggtggatt gggaggaggc
     7981 gaaggggttt gcgaaaatgg tagccgatca tctggtctcg acgattcccc agcgcttcac
     8041 ctcgaacatg tcgaaacgtg cccggaaaga aaaaatattt attgattatc ttcgcaatgc
     8101 ccggggggcg acggcgatcg ccgcattctc gaccagggcg aagccgggag ctccggtttc
     8161 tgtgcccatc gcctgggaag agctttccga gacgctgccg tccgatcatt tcacggtggc
     8221 caacttgccg gtccgcctcc agcatcttcg ccaagatcca tggaaaacgt acgacaaatc
     8281 ggcccgtcgt gtgacggctg acatgaaggc acggctcaaa tcggggctgt gatgctgctc
     8341 atgatttctt tcgggttcag cttccgaaac ctgtacgacc attgagttga gtcctccgcg
     8401 cttcacggac cgttcgtgga tacattcaat cacacggaag gaagggcaac taaaacggaa
     8461 ggagaggggc tagggagcct ctgagtgtac agcgccgatt cttcaaacca gaatcaacac
     8521 ttgctggagg gatgatgtca cgcctgttag agcttcaaag atgcggccag agctattggc
     8581 tggataatct gacgcgcggc atgatgcgca gtggagactt gaagcgccga atcgacgaag
     8641 aaggcttgcg aggtgttacg gccaatccgt ccacttttgc ccttgctctt agaagcggtg
     8701 actacgatgc cgacatcgag cgtctggcac gacaagatca tagcaccgag cagatttatg
     8761 aggagctcct cgtccgcgac gttcaggaag cctgtgatct tttcacgggc atcaatcatg
     8821 cctcggatcg cctcgatgga tatgtgagtc tcgaagtctc accgcacctt gcgcacgatg
     8881 cgaccggcac catgcgcgaa gcgcgccgat tccgcgagtt ggtcgatcgt ccaaacttgc
     8941 tcgtcaaagt cccagggacg acggccgggt tcctggctat cgagcagctg ctctatgaag
     9001 gcatttctat caatgtgacg ttgctgtttt cgttgccggc ctacgaagcg gtgatcgaag
     9061 cgtacctatc cgcattggaa cgtcgctcgg ctgaaaaacg atcgttgaaa gacgtcacat
     9121 cggtggcgag tttcttcctc agccgcatcg accgacttgt cgaccaattg cttctacaat
     9181 tggaccgccc gagcgaaggg cgtccgatgt ctgcggcgct gcgaggaagg gcggccgtgg
     9241 catctgccaa gctcgcttac cgatcattcc ggcgggcgtt cggcggcgaa cgatgggacc
     9301 gcttggcggt gaagggagca acgctgcaac gcccactctg ggccagtacg gggaccaaag
     9361 acccatcgta tcgtgacgtc atgtacgttg agccccttat cggtcgcgac accgtgaaca
     9421 cgatgccgga acaaaccatt cgtgcctttg cagaccacgg acaggtgaag ccggatcaaa
     9481 ttgagcaaga ggttgaagcg gcggctcgga caattcaggg cctatcggaa acgggcatcg
     9541 atctgcgctg tgtagcatgg caactcgagc atgaagggat ccggcaattt acggaggcat
     9601 tcgatggggc gcaccgcgaa gtggaggaga aaaggcggtt ggccgtgagc cgaccggctg
     9661 cggcctgatc ctcgatctgc tcatcctgga tggagaaggg gagcgagtga tgaaagttgc
     9721 cttcaatggt atgggacgga tcggacgggc cacgtttaaa atcttggcga ccaatccgga
     9781 gcttgatgtg gtcgcgatca atgacgtgag tccacccgac cacctggcct acctcttgaa
     9841 tcatgacacc gtctacggcc ggtatgcgaa tcgggtcaca gttcgaaacg gctcgttgac
     9901 gatggcggac cggtcgtacc ctgtgttcgc ggaaccagac ccggcgcggt tgccatgggg
     9961 cgacctcgaa gtcgatctgg tgttcgaatg cacgggacaa ttcaatcagc ggcacgacct
    10021 ctccaagcac ttggatgccg gcgcgaaacg agtcatctta tcggccccgt ccaaggacaa
    10081 cgatattccc acggtcgttt ttggcgtcaa tgtctatgat gaaaaaaacg gcgccatcct
    10141 ctcgtgcggc agttgcacga ccaactgcgt cgctccgatt gtagaaatta tcgagcggcg
    10201 actcggactc cgcaaagccg tcatgaatac cgttcatgcg tataccgcca gccagggaat
    10261 tgtcgacggc tccaaggaga acatgcggag gggtcgtgcc ggtgcggcga accttgtgcc
    10321 ctcgagcagc ggggcggcta cggcgactgc acgggcgttg ccgtcgttgc agggacgatt
    10381 cgaggcaacg gcagtccgag cgccggtccc gatctgcagc attgcggatg tggtcatgct
    10441 caccgcacgc gctacttcgg tcgaggaagt caacgcgctg tttctcgaag aggcgaggag
    10501 tgaacgttat gagggcgtcg tgggcgtgac ggaggacccg gtagtgtcta ccgacatcat
    10561 ccaagacccg cgggcgtcca ttatcgaccg cgagatgaca caggtggtcg acggtgatct
    10621 tgtgaaggtg atgagttggt atgacaatga gtggggttac gccagccaaa tggtgcggca
    10681 ggccgtcagc atggcccaaa tcgttcctgt cgaacccgc
//
LOCUS       DASTQY010000222         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1686605, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000222 DASTQY010000000
VERSION     DASTQY010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686605"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..462)
                     /locus_tag="VFR82_07285"
     CDS             complement(109..462)
                     /locus_tag="VFR82_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501439.1"
                     /translation="MRPHLLMAISGTRITSPFKLATAKSRLRSQTLDARQNGELPEAV
                     VLLIDGDNQVADVVVYPGQNSGQSAVGGVGLKVSSPVPLSGCLDCEGSDSMKQTTVTD
                     TAVARLLGRFRVVVS"
     gene            complement(574..1317)
                     /locus_tag="VFR82_07290"
     CDS             complement(574..1317)
                     /locus_tag="VFR82_07290"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU4501440.1"
                     /translation="MTSASKSRERIDTRRKNQRFHTDISWLNSYHSFSFADHYDPQNT
                     HHGLLLVSNDDIVKPNMGFRTHPHQDMEIVTWVLDGELEHKDSEGNTGIIYPGLAQRM
                     SAGTGIWHSEMNPQKDKNVHFIQMWVPPDTEGTSPSYEQLDINRELEKGSLVPIASGR
                     GHEAAISIRQKDAILWGGRLKAGYSMQIPDAPYVHLFIAKGRAELEGAGSVEEGDAVR
                     LTAAGSRRLRADSTGAEVLVWETARELAA"
     gene            complement(1344..2018)
                     /locus_tag="VFR82_07295"
     CDS             complement(1344..2018)
                     /locus_tag="VFR82_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4501441.1"
                     /translation="MGMEGKVVLMTGGSGALGQTVIPAFVSAGARVIAGDLQPLSRLP
                     AEVTAIKADVTDEADVHRLVDEAIRTSGHIDALINLVGGFAEGRVVETDISTWQRMLT
                     LNLTSAFTLSKAILPHMLERRMGRILHIAARAAVEPFPGAAAYIVSKSGLVALVRTLS
                     IELSGSGVTVNGILPNTIDTPGNRKAMPNADPSKWVRPEIIAHTLVFLASDEAVQING
                     ALIPIG"
BASE COUNT          459 a          628 c          546 g          516 t
ORIGIN      
        1 cgctgtggcg ttgcgtctgt tgtcccatta tcgtcacgga gatctcctct gcctcatgct
       61 caatagccgc cggagtttac gcatgagacc cggtttccaa aatagaaatc agctcacaac
      121 tacgcgaaag cggcctagaa gcctagccac tgcggtgtcg gtcacagtcg tctgcttcat
      181 gctgtcgctt ccttcgcaat ccaagcagcc ggatagcgga acaggagagg acactttgag
      241 accaacgccg ccgaccgctg actgcccgct attttgccca ggataaacaa ctacatcggc
      301 cacctggttg tctccatcga ttaaaaggac gaccgcttcg ggaagctcgc cattttgcct
      361 cgcatcaagg gtctgacttc gaagcctgct cttggccgtc gcgagtttga atggtgatgt
      421 gattctcgtg cctgaaattg ccattaaaag atgtggtctc atacctctct taatacttat
      481 ttcggcaagt agtggatgta ttgccgacat ctccggtgct ggatttcatt gctctggcga
      541 cttgacccga agtcagacac ctctctgttc aattcacgcc gcaagctctc tagcagtttc
      601 ccagacgagg acctcagccc cggtggaatc agctcgcagt cgtcgggaac cagccgcagt
      661 aagacggacg gcatcgcctt cttctactga accagcgcct tcgagctccg cccttccttt
      721 agcgatgaaa agatgcacgt acggcgcatc gggaatttgc atcgaatacc cggctttgag
      781 tcgcccgccc cagaggatgg catccttttg cctgatagaa attgcggcct cgtggcctcg
      841 cccggacgca atcggaacta ggctcccttt ctccaattcg cgattgatat ccaattgctc
      901 gtaactgggg ctggtgcctt cggtatccgg agggacccac atctgaatga agtgcacgtt
      961 cttgtctttc tgcgggttca tttctgaatg ccaaatgcca gtgccagcgc tcatacgctg
     1021 agccagccct ggataaataa tgccggtgtt gccctcggaa tccttgtgct cgagttcacc
     1081 gtcgagcacc cacgtcacaa tctccatatc ttgatgggga tgcgtgcgga atcccatgtt
     1141 gggtttcacg atgtcatcat tgctcaccag gaggagccca tgatgagtat tttgaggatc
     1201 gtaatgatcg gcaaagctga agctatgata ggaattgagc caactgatgt cggtgtgaaa
     1261 ccgctgattc ttccgtctag tatcgatccg ctcgcgactc tttgaggcac tagtcatagg
     1321 atgtattcct tctccttccc tcgctagccg attggaatca gcgcgccatt aatctgcacg
     1381 gcttcgtcgg atgcaagaaa gaccagcgtg tgggcgataa tctccggtct aacccatttc
     1441 gacggatcgg cgttcggcat ggcttttcta ttcccaggcg tatcgatcgt gtttggcaag
     1501 attccattga ccgttacgcc tgacccgctc agctctatgg agagggtacg gaccaacgcg
     1561 accagcccag attttgacac aatgtaagcc gccgccccgg gaaacggctc gactgccgct
     1621 cgcgcggcaa tgtgcaggat gcggcccatt cgccgttcca gcatatgagg taagatcgcc
     1681 tttgaaagcg taaaggccga cgtgagattt agggtcaaca ttcgctgcca agtggagata
     1741 tcagtttcca ccacacgacc ctcagcaaag ccgccgacaa gattgattag cgcatcgatg
     1801 tggccggagg ttcggatcgc ctcatccacc aggcgatgga cgtcagcctc gtctgtgaca
     1861 tcggccttga ttgccgtgac ttcagcgggc agcctggaca gcggctgaag atctccagcg
     1921 ataacccgcg cacctgccga cacaaacgct ggaattaccg tctgtcccaa ggcaccggag
     1981 cctccggtca taaggacgac tttaccttcc attcccatgg ttcccatatc catctaccta
     2041 cctaatagcc tgctccagcg ttgttcgaac atcggggtca tccccggttt atacgttcgg
     2101 aatcaatatc ccaccgtgaa ccgctgccgg atatgcgtcg gcttctccg
//
LOCUS       DASTQY010000223         3199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1218196, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000223 DASTQY010000000
VERSION     DASTQY010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218196"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VFR82_07300"
     CDS             <1..260
                     /locus_tag="VFR82_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU4501442.1"
                     /translation="GTTFLATRPKDNASYTALLEALSDARAEGNLGVPLHLRNAVTSM
                     MRDLGYGKGYRYVHDDPQAQREQEHLPEALKGRKYYKPKGK"
     gene            312..701
                     /locus_tag="VFR82_07305"
     CDS             312..701
                     /locus_tag="VFR82_07305"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4501443.1"
                     /translation="MKRAQSKNQSSSAAANERRKLVRATLVGSALVSPKAGAKAITAV
                     LDNVNRVGAGLHAKDKLVNGEKVTVSLAFLDSDRVEQQERLDGTVAWVKTWEKGYLIG
                     VVWDEIVTKEKNKWLYYYLEETIKSSA"
     gene            complement(726..2564)
                     /locus_tag="VFR82_07310"
     CDS             complement(726..2564)
                     /locus_tag="VFR82_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU4501444.1"
                     /translation="MLQIESVHKQFSTKVLFDGASAHLRPHTRVGLVGPNGTGKTTLF
                     KMILGEESPDEGAIRKRPRLRIGYLPQELETISGKTALDAAHRDEYPEHEAKRILSGL
                     GFSEADFSRPVDKLSGGYRMRVALAHLLLSNPDVLLLDEPTNHLDKPTQRWFEEFLLD
                     SKLTLLVISHDTAFLDRIATHIWELRDRTIQEFRGNYTKFRELRAARDAQLHAAANRQ
                     AKEVARVQQFVDRFRYQANKARQVQSRIKQLEKVKLIELQRDGKRVKFKFPLPSTSGR
                     HVLDLQGVSKRYGEKVVYTSLDFSVERGQRIALVGENGAGKSTLLKMLAGVLPPDKGT
                     RTVGHGVSLHYFAQHQAETLNPEHSILESLGEVSTTAETNFLRGLAGAFLFSGADQKK
                     PIKALSGGERNRVALARMLVEPANTLLLDEPTNHLDPASVDVLTDAMIDFPGTIIFIS
                     HDPTFLTRISTRVVEIEDGKARNFFGDYEYYLWKKAQEFEPIKESSDELGVKQGKAGG
                     PTKAMASQTAPKSSAGDRRDLTKTQARLEKQVARAEAEIAEQEAKIKERDQMLADPAL
                     YQEFSKWNGLHQEQDTWKQELERLTARWESLSSDLADVKQKLAAIA"
     gene            complement(2717..2792)
                     /locus_tag="VFR82_07315"
     tRNA            complement(2717..2792)
                     /locus_tag="VFR82_07315"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2757..2759),aa:Ala,seq:ggc)
BASE COUNT          677 a          867 c          950 g          705 t
ORIGIN      
        1 atggcacaac ctttctggcg actcgaccaa aggacaatgc gtcgtacacc gcactcctcg
       61 aggcgctgtc agatgcgagg gcggagggaa atctgggcgt tcctctgcat ctccgaaatg
      121 ccgtcacgtc catgatgcgc gatttgggtt acggcaaagg ctaccggtat gtccatgacg
      181 atccgcaggc acagcgggaa caggagcatt tgcccgaggc gctgaaagga cgcaagtact
      241 acaagccaaa ggggaaatag actggactgc cggcgttgga caaagatttc tattcggtta
      301 tacttagccc catgaagcgt gcccaatcta agaatcaatc gtcgtcagca gccgccaacg
      361 agcggcggaa gctcgtgcgg gccacgttag tcggttccgc attggtgtca cccaaggccg
      421 gtgccaaggc catcacggcg gtgctggata acgtgaatcg ggtgggtgcc gggctgcatg
      481 caaaggacaa gctcgtgaat ggcgaaaaag tcaccgtgtc gctcgcattt cttgactcgg
      541 atcgggttga gcaacaggaa aggctggatg ggaccgtggc ctgggtgaaa acgtgggaaa
      601 agggctatct tatcggtgtg gtatgggatg aaatcgtcac gaaagaaaaa aataaatggt
      661 tgtactacta tctcgaagaa acgattaaat ccagcgcata gaagatgaag tgagccgcct
      721 gctttctaag cgatcgccgc cagtttctgt ttcacatcag ccaagtccga ggacagcgat
      781 tcccatctgg ccgtgagccg ttcgagctcc tgcttccagg tgtcttgttc ttgatgcagt
      841 ccgttccact ttgagaattc ctgatacaac gccggatccg ccagcatctg atcgcgttcc
      901 ttgatttttg cctcctgctc ggcgatttcg gcttccgccc tagccacttg tttttcgagc
      961 cgtgcctggg tcttggtcaa gtcacggcgg tctccggctg aagacttagg cgctgtttgc
     1021 gacgccatgg ctttcgtcgg gccgccggcc tttccctgct tcacacccaa ctcgtcgctg
     1081 gattccttga ttggttcgaa ctcctgtgcc ttcttccaca agtaatattc atagtctccg
     1141 aaaaagtttc gcgcttttcc atcttcgatc tccaccacgc gagttgagat tcttgtgaga
     1201 aaggtgggat cgtgggagat gaaaatgatt gtgccgggga agtcgatcat cgcatccgtg
     1261 agcacgtcta ccgaggccgg atccagatga ttcgtcggct catcgagcaa caatgtattg
     1321 gctggctcta ccagcattct tgcgagtgcc acacggttgc gctcgccgcc gctgagcgct
     1381 ttgatcggct ttttctggtc ggcgccggaa aaaagaaagg caccggccag cccgcgcaga
     1441 aaattggttt ccgcggtggt cgagacctcc ccgagcgatt cgagaataga atgttccggg
     1501 ttcagcgtct ccgcctggtg ttgggcaaag tagtgcaagc tcacgccgtg gccgactgtt
     1561 ctcgtgccct tgtcgggcgg caggacgccg gccagcatct ttaaaagcgt gctcttgccc
     1621 gcgccgtttt cccccacgag cgcgatgcgt tgaccacgtt ccacggagaa atccagggac
     1681 gtatacacga ccttttcgcc ataccgtttg ctcacgcctt gcaggtccag cacgtggcgt
     1741 ccgctcgtgg aaggcagcgg aaatttgaac tttacacgct tgccatcacg ctgcagctcg
     1801 atcagcttca ctttctccaa ctgcttgatc cgtgattgga cctgacgggc cttgttggct
     1861 tggtagcgaa accgatcgac gaactgctgg accctcgcca cttccttggc ttgacggttt
     1921 gcggcggcat gcagttgcgc atcgcgtgcc gcgcggagct cgcggaactt ggtgtagttg
     1981 ccgcggaact cctggatcgt ccggtcccgc agttcccaga tgtgcgtggc gatgcgatcg
     2041 agaaaggctg tgtcatggct gatcaccagc agtgtcagct tggagtccag cagaaattct
     2101 tcgaaccagc gctgtgtggg cttatcgaga tggttggtcg gctcgtcgag caacagcacg
     2161 tcgggattgg acaagagcag atgggccaac gcgacccgca tccgatagcc acccgagagc
     2221 ttgtcaaccg gacgagagaa atctgcctcg ctgaatccca gcccggagag gatgcgtttg
     2281 gcttcgtgct ccggatactc gtcgcgatgg gccgcatcga gcgccgtttt gccgctgatg
     2341 gtttcgagtt cctgcggcaa atagccgatg cggagacgag gccgttttcg tatcgcacct
     2401 tcatcaggcg attcctcgcc caggatcatt ttgaacagcg ttgtcttgcc tgttccgttg
     2461 ggacccacaa ggccgacccg agtgtgtgga cgcaggtggg cggaagctcc gtcgaacaac
     2521 accttggtag aaaactgttt gtgaacagac tcgatttgta gcatgagccg tcaggtcaaa
     2581 attgtgaaac agagaagtgt aagaaatgga ctaaagcgaa ggaggaaggc gcatcagcag
     2641 cgggaaggcc cggtctcttc gcacccctga actgatcaac gtgagacgat ccagcatggt
     2701 atcagtgagc ttggtttggt ggagctggcg gggattgaac ccgcgacctc gtgaatgcca
     2761 ttcacgcgcg ctcccagctg cgccacagcc ccacttggtt ttctgaacaa gctgaaaaat
     2821 catgtaaaaa cgaacgtcat gctaacactg ccgtctgtac tcagtcaaga aattgggcgg
     2881 gtaacccgcg accggctttt gtgctcgcgc gacgagggcg gagggctgtt gatctcctct
     2941 gctcgctcag cgcgcacacc agatccatcg catttaatag agagggcggt gctcgtcgag
     3001 gcgcgcagta ggcgatcaat cagccctcgc cctgataagc acgaacacgc ttgaaaagaa
     3061 cgttctggta agggcggtgt ccttgggaag atggcgcgtc tcggcgcgcc cgggttgggc
     3121 gggtgagaaa gggacgcgcg cagtgcaagg cagtcgtagg ttacgcctga agggctaagg
     3181 aaagagatga gagtccagg
//
LOCUS       DASTQY010000224         4423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1066610, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000224 DASTQY010000000
VERSION     DASTQY010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066610"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..2452
                     /locus_tag="VFR82_07320"
     CDS             221..2452
                     /locus_tag="VFR82_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEU4501445.1"
                     /translation="MRHRTCPLFRGLIVVFLASMSFVQPAAAAEADWSGSWGTKWKAG
                     GARLDLNQSGDQVTGHYPLFGGEVVGKAAGTTLEGRWIEGARSGSFVFTMSPDGRTFT
                     GRYGNGEWWTGERTRLAAPTARVDRSDVRETFRTFIKGGNLAKSGLDEEIGTSVAVLD
                     FRNRPDTILQGQKVLAARELFELVSLTTFQIWELPGKLLETDIFTAKLQQAGTDVVLP
                     LSFVRGSDGQWAILMPDESAFANVRKALLARYGGRPPAPSAYLKLRTARDTMMAFQAA
                     FATWDRGGRQRVLDTLDSSQLFAATRDYEADLAAQYIKAVLDRVSNLEPQESPDDPQD
                     SQPFIVFSHPAGNIVIAPTEQPDKTAIWRFTPETLSSIRRVHAAVEPMPPLAAGTLEG
                     PHTTFFARRSWVRSVAPGLMRPIGPVEGWQILAGILGVLVCSLAAAVGTAFFLKIFQS
                     SRKRSTEAQRSLRWPLLLTTAAVLFKLLIPLYGWPEEVRQITAPIHALIIGVFGAWAA
                     WELINMTTQGLLSRARRTHSYLDDMTITLVGGAARICVLFVAAAYVATQLAIPTNGIL
                     AGLGLGGLAVAIASKETLSNLFGAGILVADRPFNKGDLITVGDIQGTVEHVGIRSTRI
                     RTAEDTQMIIPNGKLSDASINNWGHRKYRLFETKVRVPYGTTASKVEAFQAKLRRLFA
                     EDEAVAADRTEVGISSLSDTGMVIDVSTYFRAAPTERAVKNRLMLAVIQLAEQENVVI
                     AGT"
     gene            complement(2724..2909)
                     /locus_tag="VFR82_07325"
     CDS             complement(2724..2909)
                     /locus_tag="VFR82_07325"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEU4501446.1"
                     /translation="MATAVSVTVVGAMILVSGIAEIVSAFRVRTWGKFLLEMLLGALY
                     VIADLLTFQNPSWLPAS"
     gene            3241..3942
                     /locus_tag="VFR82_07330"
     CDS             3241..3942
                     /locus_tag="VFR82_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501447.1"
                     /translation="MAQQAGVSRARLRTPKAAAVAGVIFSILLIAIFWLLRSSLPADP
                     MQPGTWLAGNTAPATLALNLIPFAGIAFLWFIGVLRDRLGQLEDRFFATVFFGSSLLF
                     IGLLFVAATLVGALVVLSQFHEPHDAMSITTFRFGRAAAYILVNIYAIKMGAVFMIST
                     STVIIYTRIVPRWIAVTGYALALLLLIGSYGPNWSVLVLPVWVLLVSVHILLDEFRRT
                     GEPPTSAGPADQTRP"
BASE COUNT          903 a         1286 c         1307 g          927 t
ORIGIN      
        1 catcccgctc gcggatccca aggaagcccc cggcgccgat tacgatggaa ttttttcatc
       61 cgggccctgt aggacccgct cgacatcgcc gagctcatcg ccgcggtcgt tatagaggtt
      121 caacgccgat ttgattgtgg gcggtgggcg ggctcagcca tgcgtttgcc ttcttctctc
      181 gaaatacgag ccaggagtag atttgctggg agtgctgccg atgaggcacc gaacctgtcc
      241 tcttttccgc ggactgatcg tagtctttct cgcctccatg agctttgtgc agcccgccgc
      301 tgcggctgag gctgactggt cgggctcgtg gggcaccaaa tggaaagccg gcggcgctcg
      361 gctcgatctt aaccagtcgg gcgatcaggt cacaggacat tatcccctct tcgggggcga
      421 ggtggtgggc aaagcggcag gaacaacact ggagggtcgt tggattgaag gggcacgctc
      481 aggctcattc gtcttcacga tgagccctga cgggcgcacg ttcacgggac gctatggcaa
      541 tggcgaatgg tggactggcg agagaacgcg ccttgcggcg cctaccgcgc gggttgatcg
      601 atccgacgtc cgagagacat ttcgcacctt catcaaaggc gggaaccttg caaaatctgg
      661 tctcgatgaa gaaatcggga cgtcggtcgc cgttctcgat tttcgtaacc gtcctgatac
      721 gatcttgcag ggccaaaagg tcctcgccgc tcgcgagctt tttgagcttg tcagcctcac
      781 gacatttcag atctgggagt tacccggcaa gctacttgag accgacatat ttacggccaa
      841 gctccagcaa gcgggaaccg acgttgtcct acccttgagc ttcgtgcgag ggtcggatgg
      901 ccaatgggcg atcctcatgc ccgatgaaag cgctttcgcg aacgttcgga aagcacttct
      961 tgcgcgctat ggcggccgac caccagcacc ctcggcctac ctgaagctac gcaccgctcg
     1021 cgatacgatg atggcctttc aggccgcctt cgcgacctgg gatcgcggcg ggcggcaacg
     1081 tgtgctcgac acgctggata gctcacagct gttcgcggca acgcgagatt atgaggctga
     1141 tctcgccgcg caatacatca aagcggttct cgaccgcgtc agcaacctgg aaccccagga
     1201 gagcccggac gatccgcaag acagtcagcc cttcatcgtc ttctctcacc cggcgggcaa
     1261 catcgtcatc gctccaactg agcaaccaga taaaaccgct atctggcgct tcacccctga
     1321 gacgttgagt tccatccgaa gggtacatgc ggcagttgaa ccaatgccgc cgttggctgc
     1381 gggaactctt gagggaccgc acacgacctt ctttgcgaga cgctcctggg tgcgctctgt
     1441 agcacccggg ctgatgagac cgatcggacc ggttgaggga tggcaaatcc tggcaggtat
     1501 tcttggtgtt ctcgtgtgct ctcttgccgc ggccgtagga actgccttct tcctgaagat
     1561 tttccaatcg agccgcaagc gatccaccga agctcaacga tcgctgcgtt ggccgctcct
     1621 tctgacgact gccgctgtgt tgttcaagtt gctcatcccg ctctacgggt ggcccgaaga
     1681 ggtgcggcag atcacggcgc cgattcacgc actgatcatc ggtgtgttcg gggcatgggc
     1741 agcttgggag ctgatcaaca tgaccactca gggcctgctt tcccgggcaa ggcgtaccca
     1801 ttcatacctc gacgacatga cgatcacact agttggggga gccgctcgga tctgcgtgtt
     1861 gttcgttgct gccgcctatg ttgcaacgca attggcgatc ccgacaaacg ggattctcgc
     1921 gggcttggga ctcggcggcc tcgcggttgc tatcgcctcg aaagagactt tatcaaatct
     1981 gttcggcgcc ggaattttgg ttgcggaccg gccattcaat aagggcgatt tgatcaccgt
     2041 cggggacatc caggggactg tcgaacacgt cggcatccgc tctactcgca tccgcacagc
     2101 ggaggacacg cagatgatca tccccaacgg gaagctctca gacgcttcga tcaacaattg
     2161 gggacaccga aagtatcgcc tgttcgagac caaggtccgg gtcccgtacg gtacgacagc
     2221 gtccaaggtc gaggcctttc aggctaaact gcgccgcctc ttcgccgagg acgaggccgt
     2281 tgcggctgat cggacagagg ttggcatcag cagtctgagc gatacgggga tggtgatcga
     2341 cgtctcgacc tacttccggg cagcacccac tgagcgagca gtgaagaatc ggttgatgct
     2401 tgctgtcatc cagctagccg agcaagaaaa cgtcgtcatt gccggaacgt gacttctatc
     2461 cgctcagtac gaacgagtcg acgggctgtt gtggttgaga gggggttggc atcaggacga
     2521 aagcatccag cgatcgaaac gcgattgcga tgtgaccgac cctcctgatc aatctaccgc
     2581 cgcgcaggga gccgaacacg atgccaagcg tgatgatgcc ggagagcgca acgcaccccc
     2641 aaaggcgtcc ccctcattca tttgcatcgt cagcaagatg cgcacgaaac cggaaacgat
     2701 cagggcaaat cggagcatca gtgtcaggat gccggcagcc aggatgggtt ctggaacgtc
     2761 agcaggtcag cgatgacgta aagggcaccg agcagcattt ccaggaggaa cttgccccag
     2821 gtcctcacgc ggaaggcact gacgatctca gcaattccac tgaccagtat catggcgccc
     2881 acgacagtaa ctgaaacagc ggtggccatc gccccactgg gacggcaata aggccggcga
     2941 caaggaaagg cgcgcgtggg tcggagacat tgcgagttga tcggagcagg tcacgcacgt
     3001 ggtgtcgaac atgacctggg cagttcggga cctcggcagc gcgagaccga ccagtaccat
     3061 ttcctaccag ctacggacaa agccgacgat tgtcgctcca tgaatgctga gcacgtcgta
     3121 gcagccaatg ggggatatcc cgcctaagcc gccaacttga cattaatcaa gttggtcgcc
     3181 ggtaggcgcc ttagtttcgc aaagaaagtc tggccttcgt taggcacaac tgacagttcg
     3241 atggcacagc aggcaggcgt gtcccgcgca aggctgcgaa ctccgaaggc tgctgctgtt
     3301 gcgggggtca tcttctcgat tcttcttatt gccatcttct ggttgctccg gagctccttg
     3361 ccggctgacc cgatgcaacc tggcacgtgg ctggctggga acaccgcgcc agcgactcta
     3421 gcacttaatc tcatcccctt tgctggcatt gcgttcctct ggttcatcgg cgtgttgcgc
     3481 gatcggctcg ggcagctgga ggatcgcttc tttgcgacgg tgttttttgg cagcagtctc
     3541 ctctttatag ggctgctatt cgtcgctgca acgctcgtcg gggcactggt ggttctgtcg
     3601 caattccacg agccacatga tgcgatgagc atcacgactt tccgtttcgg acgtgcggcc
     3661 gcctacatct tagtcaacat atacgcaatc aagatggggg ctgtcttcat gatctctacc
     3721 tccacggtga tcatctacac ccgaattgtc ccacgctgga tcgcagtgac agggtatgcg
     3781 ttggcattgc tccttctcat cggcagctac ggcccaaact ggagcgtctt agttttgcct
     3841 gtatgggtct tgctggtcag cgtgcacatc ttgctcgatg agtttcgacg cactggggaa
     3901 ccgccaacct cggctgggcc tgccgaccaa actcgccctt gacggccgga ctcgcaccct
     3961 accacggcaa gagcaaaggc atgagccgga cgctgccctt gcgcgagggc gagcgtccta
     4021 gcgacaacga aactgtcccc tgcttcgact gcgcttttgg agggtggctg ccatgtggcg
     4081 aatgcggtct cggtgagaga gcttttgaca aaagtcaata tgctcacccg cccctactgc
     4141 tttcgacgtt cagcaattat ggagaatagg gatctccgca gtgattgggg ccgtgtcttt
     4201 gataatgtgg tacaaacagc gcgtaaccat gtgagtagga agacggaaaa tgtccgccca
     4261 ctgcgcagtc aatgacgtgt cgcaagaagg caagagccta aggttgcttc aaagcgaaaa
     4321 gcgcgtgctc ggccaggtcg cagctggcgt agccctctca aaggcgctcg aagagttact
     4381 cctcgccgtt gaagcagaat ccaacggcga gatgtgtggg tcc
//
LOCUS       DASTQY010000225        10080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1344073, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000225 DASTQY010000000
VERSION     DASTQY010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10080
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344073"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2887..3138)
                     /locus_tag="VFR82_07335"
     CDS             complement(2887..3138)
                     /locus_tag="VFR82_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501448.1"
                     /translation="MTVGMAGTRPTKVELSRDDQPPFAVWPTGTFFTLSADGQSLTGN
                     WCITSSCWGNVAGEHVLNVVATYATGERATAAAALNVAD"
     gene            complement(3820..4341)
                     /locus_tag="VFR82_07340"
     CDS             complement(3820..4341)
                     /locus_tag="VFR82_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEU4501449.1"
                     /translation="MGLIVKVSQTASGFSAFFLLCEILWFVVDSTATEFSSLVVSVID
                     GDTIEVLHYEKPERIHLNGVDCPEKGQTFGQRAKQATSELVFGKDVRLQTHGTDKHGR
                     TIADVFLPDGRKVNQILVKNGWCWWYQKNAPRDVILEELERRARAERSGLWGDPHPVP
                     PWLYRQLTADAYP"
     gene            4821..5267
                     /locus_tag="VFR82_07345"
     CDS             4821..5267
                     /locus_tag="VFR82_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501450.1"
                     /translation="MTAPQFQMMEQPVTIAKENITLNGFLSVPHLATGIVIFAHGSGS
                     GRFSPRNRFVAHHLQQERIATLVVDLLTTDEAQDRRNVFDIDLLANRVLMASMWLRED
                     ARTKVLALGYVGASTVQPPRSKRHRLSFHKLHDPKPPLDCRIPLTA"
     gene            complement(5775..6131)
                     /locus_tag="VFR82_07350"
     CDS             complement(5775..6131)
                     /locus_tag="VFR82_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501451.1"
                     /translation="MTLVKSTGAVMAVMSLTVATTLFAATIEAPSKSGSPTSKTVRGE
                     VSVVEGEFHMAKNSQGEDILQMVDKSYVITTPTGQEIPLKLSRDTKVPTRANPGDRVE
                     AKISDRGQTLSVMLVE"
     gene            complement(6444..6821)
                     /locus_tag="VFR82_07355"
                     /pseudo
     CDS             complement(6444..6821)
                     /locus_tag="VFR82_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            7355..8992
                     /locus_tag="VFR82_07360"
     CDS             7355..8992
                     /locus_tag="VFR82_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEU4501452.1"
                     /translation="MLSSVSSINRGLCLIFIISLTGCGGGDESVPNGALSNGPSTGVG
                     APTPLVPRCDATASGTSTVEKPELLTKLADRYHEGWLASPAVADLNGDGTNEIIILRA
                     ERVETYGPKGNLLWTYSFTGRAWASPIVANLVGDSALEIAFAARDKVFVLDKAGQVLP
                     GFPVTWQNEMRALAAGDVDGDGKLDLVASVGHPAPITDVVNAWHTDGTHVPGFPPNAR
                     GVSGCDAHCYLAGGYDQNVALGDFDGDGKADVIVPQDNAYVSIHKDTGEAFNANSMFK
                     PQKVIGVRFMHDLALAIQGYANNENTDLQAHFTNTAPAIADINGDGAYEAVFTGSVQN
                     ASQSDRFKGVGLWAIRSDASRIHGWEAPFHAPHYLSGLWDSGNNIVGLTNQVTIADIN
                     ATKPGLEMIFAGFDGRIHAVAADRTELWQMQYTTSTGVFTPGIVVADLSGDGIPEIVF
                     ATYSSAENGSALFILDATGKKLFEEPLPNRGSMAVPTLADVDGDGTVDIVISLKDAVD
                     KVELARIYRVPGSKTNCLVWPTGRANNLRNAWVPKKN"
     gene            complement(8989..9240)
                     /locus_tag="VFR82_07365"
     CDS             complement(8989..9240)
                     /locus_tag="VFR82_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501453.1"
                     /translation="MRGPQQIQAWSFSGRLCVHRSIARISGYTVSHQPSGRAVLTRIR
                     DKAAAFDLVRRLLALGEIDWHADRPLENPAALDAMLAVS"
     gene            complement(9265..9678)
                     /locus_tag="VFR82_07370"
     CDS             complement(9265..9678)
                     /locus_tag="VFR82_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501454.1"
                     /translation="MGEMKQRMRYFFVDEDEVIHKIPFAKYERIFRRAQPIMLFAGRS
                     VRFIEAHVEVDEGGVEHLSNAFFRRHGFDEQGLWDKREKQKSMLGAMEMLSHRGDADW
                     TEFYRSEHLDPFTWKPTPELIERLKAAVRDRKIHS"
BASE COUNT         2413 a         2652 c         2639 g         2376 t
ORIGIN      
        1 tctactagct caacgtcaac gtcgagtggc tcaacctcaa cgagtacgag tacttccagt
       61 agtacatcga acacgaccgc gaacctaagc tggtatccca acgctgagac gaatcttgcg
      121 ggttataaag tccacatcgg cactgctgcc ggaatttata attctacagt ggatgtgcgc
      181 aatgtcacat cgtataatct gtccaatctt gctataggga ccacgtatta ttttgcggtg
      241 agcgcctacg ataccgctgg cactgagagt gctttctcga atgaagtgag caagagcatc
      301 tattaaagca gctccgttgg gcccttccca ccctattagc acacggactc agtaagtaag
      361 ggcactgacc cgggtacctt tgtcgtgttc cccggccctt cttctttatc ctctgtttat
      421 gtgactgtta aatctagaag agatcgtgcg gaaccacatc gataggtaaa cactgtctcg
      481 agggaagatt ggcctctttc acgtcggcgt agtattcggc tcgcccacac ccagtgcagt
      541 gccatgcgga cactgtttta agtccttcca acccattcga aagatcgaaa acggttttgt
      601 gcataccgcc ctggcagcgt tcgcatgtca tggacactat cccccggtga gtcggcctac
      661 actcagccac gcaaggccca tgccgtctct gctgtcacaa catcgcgaaa caacaagccc
      721 tacattggat gagcgcttct aaacggatat cttgtccaac aatgtcggac gaagtgtccc
      781 ttcgcatgat ggacactccg ctcgttgatt taatcacgcg cggtcagtga taggtcgaag
      841 ggagaaattg accgcaagcg aacatttgag acgtataaat gactatcatc tcgacaatct
      901 caggacggta gcgtgttggg ctcgtcagct tagctatcga gaagaatcta atgacgactg
      961 cggaaataaa aacggccgtg gcaccgtacg cactgccagg gataccatcc tacccagctc
     1021 agcgcatgat cggtccatcc atacctgctc acccgttggc atctgcaccg acacgaaggg
     1081 tgggatttag taggaattgt ggagaaacga ggaagccgtg cacgagcgtc cgtcagggca
     1141 cgatcaatca aggaagttag aaaagaggtc gatgtaataa gaaagtcacc agccaatttc
     1201 atgccggaga atatactgtc ctccatgagt aatcgtgaat tggggagcct acgtagacgg
     1261 gtcgtgtcaa ctctcttaca ggagctggcg gtctaagggc tgcggctgcg tcatgcgctc
     1321 gtctccgaat tcacaggccc agatcgaaag agtctcgcac atagttggac caactgcttt
     1381 cttgcaaacg gcttggccaa atacgcattg gcaccgctgc tcaaggcctc gtcgaacatc
     1441 agtctgtcgt gagaggccga aatcattaag atcggaagat ccggatactg ctttcgcaac
     1501 caccggagca cctcaatgcc atgcacaacg ggcatttcca tgtccaagat taccagtgat
     1561 ggggcttggg cgggcttcca aagaaacgag cgcaagcgct gtttgaccgt cgtgggcggc
     1621 aatcacatcg taaccttccg cctccagcga attttgtaca atcgagccca ccataccggt
     1681 gctctcatcc gatgggtacg ccgattcgct gggtcaatgc cacggcttct gtccatagtg
     1741 ttcgacaaga cgcatgcaat atcgatttgc ttggtcctgt ttttgcttcc taatattcca
     1801 gttaaaagga ctttcgactg ggtaacaaag tactaggcca gttgtccagg caaggaactg
     1861 gatggcgtac cttatgcagg cctatgcagc tactaacggt tcttctcata gacgacaatt
     1921 gtgaggacag agcgtactgg tcttcctcgc taaaaaaccc tcctattggg ttctcggtgt
     1981 tggaggccgc ttgcggagag tctggattca agctgtgtca aagccagcat gttgattgtg
     2041 tcgtcttaga tctcgaaatg cctgagagcg ggttgtttac cttgctcaaa ctcattcctg
     2101 atccaacgcg gcgtcgtgtc cccgtggtca tcctcacgaa gctcgcacag ccagccttaa
     2161 ttaaattgat gaaaagtctt ggagcttatg agtgtctggt caaagaatat tgttcggcgg
     2221 aaaaccttgc gaatacaatt cggcaggcaa tagccgttgt caaatctgag cgtgctatgt
     2281 aaagtcagtc gagctcaaca aacctacccc ccttagagca ccagaactcc agagcgtttc
     2341 aggtattata agggcgggca agataacgac ctcggccaat gcacaatcga acttcacaca
     2401 agttgtaatt ctcttaagat tctgatcctc acctccagcg gcatctcgta gtccgtaatt
     2461 gccgtcttta taaacacaca attagggagg ttgtgcttcg cgagtaacag ccggccttct
     2521 tcatttgatc gacagcctcc tgcgagccgc cgagagacgg aaagtggatt tgcagcaaag
     2581 cctttaaggc ctggcacacg agtggatgat catcaacgac ataataattt gatggcgatg
     2641 catcgaggcg cagtgcacaa taaacacggt attgcggatc gtcgtcgatg aagaagaacg
     2701 atagtggcgt cagacacgac aacccctttc cggtaaattg ggcttgatcg tcccttcacc
     2761 ggaaccttga agtgaaaacg cctaacctat gcgtctaata agtaggtgcc aattagcgga
     2821 gaagtagcga tggcagccat tttgcggcaa acgtgcagtt ttgtcgccag gcgggtggcc
     2881 ccgccattaa tcagccacgt tgagcgcagc ggcagcggtg gctctctctc ctgtggcata
     2941 ggtcgcgacg acgttcagca cgtgttcgcc tgcgacgtta ccccaacacg aacttgtgat
     3001 gcaccagttc cctgttaacg actggccatc tgccgacagc gtaaaaaatg tgcccgtggg
     3061 ccacacggca aagggaggct ggtcgtctcg tgatagctcc accttcgtcg gtctcgtgcc
     3121 ggccattcca acggtcacac gggcggtcgt cccattgaat gggagattcg gcgccccagc
     3181 gacccctaga ctcaccactg cagcgggttg tggcgaagcg ctaggagggt aatacgtcag
     3241 gacattgctc ggcgcggatt cgttttgcgc gaagtcgtac gaagtaacca catagtgttg
     3301 aatgactgag ggtgttccga tattgaaact cgtaactttc cccagcgtag caagcagtgt
     3361 cgctgttcca aataatttcg aacagggtag ttgactgcac tggtagacct gataccccgc
     3421 tagatcggac gagctattgg catcccatgt cagcgaggcc ccccatacag tcgtagatca
     3481 caaggctaga acggcagcgc cggcgttttg ccacttcata tggatactcc ttgatgttct
     3541 gctcttagcc ctcagaaggc ggtgaaagcg tgtatctgat aaaaaacgga gacgaaagta
     3601 gacacaccct gatgacctag gtatctggca cagcacggac tgttgagaga tccaaggtaa
     3661 aggcggcaag cggaccacaa tctttaccgg ttaagctgta atccgtcggt tcagggtgtg
     3721 tgagatacgg gattaaggat ctacggcgaa ccgagcgcag tgctgaagtt tttcagccca
     3781 gagggcagcg gctattgctt gagcaatagc cgcagggatc tacggatatg cgtctgcggt
     3841 aagctgacgg tataaccacg gcgggactgg atgaggatca ccccaaagcc cggatcgttc
     3901 tgctcgcgct cttcgttcca actcttctaa gataacatct cttggcgcat ttttctgata
     3961 ccaccagcac cagccattct tgacgagaat ttggtttact tttcttccat ctggtaggaa
     4021 cacatccgcg atcgtgcgac catgtttgtc cgtgccgtgc gtctggagcc tcacatcctt
     4081 gccgaacacg agctccgatg ttgcctgctt cgctcgttgg ccgaacgtct ggcctttttc
     4141 cgggcagtcg actccattca ggtggatgcg ttcaggcttt tcgtagtgca acacttcgat
     4201 ggtgtcgccg tcaatcacgg agacgacgag gctggagaat tcggtcgccg tactgtcaac
     4261 tacgaaccat aagatttcgc ataacaggaa gaacgcgcta aatccacttg ccgtttgtga
     4321 taccttcaca atcagaccca tgtcccccgg atgaagcgac tcgcacacta gtcattcaat
     4381 tcatagttgt agcggagccg aaatcatcac tttccaggcc ttggtttatc accagagaca
     4441 aagactgcaa tgcatgggcc actcgcgctc attcttcgaa gggacttgat ccgttgaatc
     4501 tgcaaggctg cgtagggggt ttggcattgc tcaattcaac cgggggtgga aatggtgatt
     4561 tctaaggcgg atactgactc gaaatcgatt ctctctgaat gtgaaatgat ccaggagaag
     4621 ccctcggggc taaaagagtg gtttggaaga gggaacaagc gtgaacccaa ggggacggac
     4681 aggacaggaa aaacttacgg attgcagata cgagatgaag tctctgggct ggccgtcagg
     4741 atcaggccgt aagaaagata ggtaaggtta gataatacag cgatgagcta aacttgtgac
     4801 gcagatatcc ctgttgaaat atgacagccc cacaattcca aatgatggag cagcctgtca
     4861 caattgcgaa ggagaatatt acgctcaatg gtttcctcag cgttccacat ctggcgacgg
     4921 gaatcgtcat cttcgcgcac ggcagcggaa gcgggcggtt cagtcctcgc aaccgcttcg
     4981 tggcccatca cctccagcag gaaagaattg ccacgttagt agtggatctg ttaacaacgg
     5041 acgaagcgca agatcgccgc aacgtattcg acatcgatct gttggcgaat cgcgtcctca
     5101 tggctagtat gtggctgcga gaggatgcaa ggacgaaggt gctagcactc ggctacgtcg
     5161 gcgccagcac ggtgcagccg ccgcgctcca agcgacatcg actgagtttt cataaactgc
     5221 atgatccaaa gcctccgctt gattgtcgga ttccgttgac ggcgtgatag cgaaggtttt
     5281 aggagcaagg ttccgtacga gtaaaagcaa cgttagcaag agctgtgtcg atcgtactgg
     5341 ctagttcatc accagagcta cgagctttaa acaaatacgc gtgtgctcca ttccttaaag
     5401 caaggtcctt catgggtgca agggtgagcc gtgtaaaaaa gatgaaggct atttctggat
     5461 tgcgggggtt aagaaccatc tgggagagta cttcgaaacc cgacatgtca gggagcgtca
     5521 attccgagac aatgcactca acccgctgcg accggcaaat cgctagccct gcctttccag
     5581 tggaagcttc gagaatcaca taatcgggtg aggacattct cagccgatct acccaatact
     5641 gccggtcgtc gtcatggtga tcgatcaaga gaatggttgt ggagagatga tcgagtggca
     5701 tgaaggcacc tccctccaag aaactagtga ggtttgcgat tgaagccttg tattgcactt
     5761 gctcaggatc gactttactc gaccagcatg accgataggg tttggcctct gtcggaaatc
     5821 ttggcctcga ctcggtcacc aggattggct ctggttggga cttttgtgtc acgagagagc
     5881 ttcaacggga tctcttggcc cgttggagtg gtgatgacat aagatttgtc taccatctgg
     5941 agaatgtctt caccttgaga attcttcgcc atatggaact ctccctccac cacagagact
     6001 tctcctctga cagttttcga ggttggcgat cctgatttag acggggcctc gatcgtcgca
     6061 gcgaacaggg tcgtcgcaac agtcagactc atcactgcca tcacggcacc ggttgatttg
     6121 acgagtgtca taatcacctc ccggcttgag gctaccaata gcggcttaac tcaaatgcag
     6181 cttgaatgcc aaggaagtat gtaggtggtc ttattagata actgaatcag atatgattag
     6241 aaaaaccgca agatggtgtt ttgtttctca tacaaaatgt aggcgacaga ggctagtgaa
     6301 acctctgggt gacggttttc gagtattcag tgtaattacc taaataatat tgctgttgaa
     6361 tgtcaacttg atgtaaagtc taggaccccg cattcaagtt agagctgtag cacgagcgcc
     6421 agtttggttt tgtttggtgg tcttcagtcc acttttttat ttagctcgat tgagacccca
     6481 tggtgacggt ggtaagtgtg atagtatgca agcacgttct ggatgtagac cttcgtttcc
     6541 cgaatcctcg gtacgtcgcg cttcacgcga tggactcctg cattatacgc cgcgagtgcc
     6601 agccgcagat ttccattgta gaaattcaat agataccgta agtgcttcgc gcctccttgg
     6661 atattttgaa gtggatcgtg gatgttagcc acctgtagcc tagccgccgt tggcggcata
     6721 agttgcatga gacccacggc tcctcgtcgc gagatggcat cctgccgaaa attcgactcc
     6781 accttgatga cggctcgcag cagtgcggga tctaaaccat agagcttagc gtatacggtg
     6841 agaaacgctg gcgcggagta tctggcgctg cgaaattccg ggtccgaaaa ccatggccgc
     6901 gctgattcga cgtctgcaag ctgacgtccc gccttttacg gtccgggtgt acagacattg
     6961 caagaaaatt cgccgcccga ggcacaatta ttacgcaaga ccaagcatca aactattaac
     7021 tccgactttg agcgttcgtc ttttgacacc ctcttccaga gatatttcaa tataaagcca
     7081 gggttacttc cagtgcagcg tcgcctgatg tccgtttatt taatctaccc acagtttgct
     7141 tacacgttct cgatcgtttt tagaggcgct acagttttag gaccttacat gccgatccga
     7201 cacgattgcc gctacacatc gagcccctcg caaactgatc cccccgctcc ttccttgtaa
     7261 tcgtacagcc gctctgcaag cccatttttc aaccttaacg ctaggcgtgt ggcagtcttt
     7321 ctttgccaac cctgacgagg aagtagctaa gactatgctc tcttctgtgt cttcaatcaa
     7381 ccgcggactc tgccttatct tcatcatctc gctgaccgga tgcggaggag gcgatgaaag
     7441 cgtcccaaat ggagccctca gcaatggccc atcgacgggt gtcggcgcac cgactccatt
     7501 ggtacccagg tgcgatgcga cggccagcgg cacatccacg gtggagaagc ccgagctctt
     7561 gaccaagctc gcggatcgct accacgaggg atggctcgct tcaccggcgg tcgcggatct
     7621 gaatggcgac gggacaaacg aaatcatcat cctccgcgcg gaaagagttg aaacctacgg
     7681 accaaaaggc aacctgctct ggacttattc attcacaggg cgagcatggg caagtcccat
     7741 cgtggccaac ctcgtcggtg attcggcatt ggaaatagct tttgccgcgc gggacaaggt
     7801 gtttgtgctt gacaaggccg gtcaagtatt gcctggtttt ccggtcactt ggcaaaatga
     7861 aatgcgcgcc ctcgcggccg gtgatgtcga tggcgacggc aaactcgatt tggttgcgtc
     7921 agtcggtcac cctgctccca tcacggatgt cgtcaatgcg tggcataccg acgggaccca
     7981 cgtgcccgga tttcctccca atgcgcgagg cgtgagcggt tgcgacgctc actgctatct
     8041 cgccgggggt tacgatcaga atgttgctct cggcgatttc gacggtgatg gcaaggccga
     8101 tgtcatcgtt ccgcaggaca atgcttatgt cagcattcac aaggacacgg gcgaagcatt
     8161 caatgccaat tcgatgttca agccccaaaa agtcatcggc gtgcgcttca tgcacgacct
     8221 cgcgctggcc attcaaggtt atgccaacaa cgaaaacacg gatctgcagg cgcatttcac
     8281 caataccgcc ccggccatcg ccgatatcaa tggggatggc gcttatgagg cggttttcac
     8341 gggcagcgtg cagaatgcgt cccaatccga tcgcttcaaa ggcgtggggc tctgggcaat
     8401 acgatcggat gcaagtcgaa ttcacggctg ggaagcgccc ttccatgcgc ctcattatct
     8461 cagtggctta tgggattcgg gcaacaacat tgtgggtctg acgaatcagg tgacgattgc
     8521 cgacatcaat gccaccaaac cgggcctcga aatgatcttt gccggattcg acgggcgaat
     8581 tcatgccgtt gccgcagacc gcaccgagtt atggcaaatg caatacacca cgagcaccgg
     8641 ggtctttacg ccaggaatcg tcgtggccga tttgtctggc gacggcattc ccgaaattgt
     8701 ctttgcgacc tattcgagtg cggaaaacgg atcggcgttg ttcattttgg acgccacggg
     8761 caaaaagctc ttcgaggaac cactgcccaa tcgcggatcc atggccgttc ccacgctcgc
     8821 cgatgtggat ggtgacggca cggtcgatat cgtcatcagc ttgaaagatg cggtcgacaa
     8881 ggtggagctg gcgcggattt accgagttcc cggctcgaag accaattgct tggtctggcc
     8941 gactggacga gcgaacaatt tacgtaatgc gtgggtgccg aagaaaaatt agctgaccgc
     9001 gagcatggcg tcgagcgcgg ccggattctc gagcggccgg tccgcgtgcc agtcgatctc
     9061 gccgagggcg agcagcctcc ggaccaggtc gaaggctgcc gccttgtcac ggattctggt
     9121 caacaccgca cggccggacg gctgatggct gaccgtgtag cctgagatcc tcgcgatgga
     9181 gcggtgcacg cagagccggc cgctgaagga ccatgcctgg atctgctgcg gacctctcac
     9241 ggacagaaca cggacacgat gttctcagct gtgtatcttc ctatcgcgta cagctgcttt
     9301 cagccgctct attaattcag gtgttggctt ccacgtgaat gggtcaagat gttcagatct
     9361 gtaaaactct gtccagtccg catctccacg atgggacagc atttccatag cgcctaacat
     9421 gcttttttgt ttttcccttt tatcccacaa accttgttca tcaaaaccat gtcgcctgaa
     9481 aaacgcatta gatagatgct caacaccgcc ctcgtcaacc tcaacatgtg cctcgataaa
     9541 tcgaacagac cgtccggcaa agagcattat tggctgagcc cttctaaaga tcctttcgta
     9601 cttggcgaac gggattttat ggattacttc gtcttcatcg acgaagaagt accgcattct
     9661 ctgcttcatt tcacccatga gtatcgccgt tcgtgacgcg tttcttcgct tcatctttat
     9721 ccaccctagg gtttcaaaca atccgtcgaa ggtaaggcgg cagagcagcc gcactcgatc
     9781 gcttacgcaa attgcctagc aaagctttca ctcggctgtc cgaagggcag gaattgagga
     9841 cttgcgcttc cacgacctgc ggcactgtgc agccacgaat ctaagacgcg ctgggataga
     9901 tacagtaacc gcaatgaaga tcgtgggaca taagtcggag aagatgcacc gccgctataa
     9961 ttcggtctct gaggaggatt taaaaaggca gcaggaaggc tgaacgtgta tctttctaac
    10021 acgctaataa caccaaccca atcatcccac caacccactt ctgtaagtct ttgattaatt
//
LOCUS       DASTQY010000226         9574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_902291, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000226 DASTQY010000000
VERSION     DASTQY010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902291"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /gene="rmuC"
                     /locus_tag="VFR82_07375"
     CDS             <1..359
                     /gene="rmuC"
                     /locus_tag="VFR82_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU4501455.1"
                     /translation="DGFAQGVVLATPTTLMALLRAVAYGWRQERLTAHAEEAGRLGKD
                     LYERMALLAEYVNDVGLSLGKSVLAYNKAVGSLESRILPAARRFKDLGVSSDKDISSL
                     SPIEVIPRKALSYEQE"
     gene            346..798
                     /locus_tag="VFR82_07380"
     CDS             346..798
                     /locus_tag="VFR82_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEU4501456.1"
                     /translation="MSKNEQTAPPTAQSMVEEFHRLFSIVVHQTPTVIDEQTRILRER
                     LIHEEFEELKEAMAKNDLFAVAKELADLLYVVYGTAVSYGIDMEPVFREVHRSNMSKV
                     GGYKREDGKWVKPATYSPAMIEPILTEQQAQPGREQTSQPLAEMKSGA"
     gene            795..1304
                     /locus_tag="VFR82_07385"
     CDS             795..1304
                     /locus_tag="VFR82_07385"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4501457.1"
                     /translation="MKTLHCPNCGTPFVRVAPQSGVVERVLHHLNVVPFRCQLCMNKF
                     HAFSREATRSAQASDRRQYKRLPTSIQAQFLADNRIRSTNRITDISMDGCTLQTTGLP
                     RGTFLGLVLKPEQEDEAIRIETAMVCSVRPTSVGLRFLEIPLPDQRRLSQVVLGLLVS
                     QGHHPVSNA"
     gene            complement(1432..2289)
                     /gene="panC"
                     /locus_tag="VFR82_07390"
     CDS             complement(1432..2289)
                     /gene="panC"
                     /locus_tag="VFR82_07390"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEU4501458.1"
                     /translation="MKTIRSPSAMTAWSNQLHREGVVIGLVPTMGALHDGHRALIRAG
                     RRQCDALVVSIFVNPTQFAPTEDLATYPRPIEKDRALCRHEGVDVCFEPAADAMYPKG
                     FQTLVTVPSIARRWEGESRPHHFTGVATVVAKLFNVVRPGIALFGQKDYQQCGVVRQL
                     VKDLNFPTRVVVCPTVRERDGLAMSSRNVFLSTEDRRIAPIFFHTLQAGRKAIENGLH
                     DAASVEALMRKVIGGESAIRIDYLAVCDPTTLEPLIHIERQAIILGAIRLGSVRLIDN
                     VPARRKNGS"
     gene            complement(2286..2843)
                     /gene="folK"
                     /locus_tag="VFR82_07395"
     CDS             complement(2286..2843)
                     /gene="folK"
                     /locus_tag="VFR82_07395"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU4501459.1"
                     /translation="MECETAYIGFGSNVGDRIDFCDRALTLLSLLPHSQVTGVSLLYE
                     TEPVKDGANPGVGWFLNGVVQLETAITPESLLSMLREIERSLGRDDDNRSGPRTIDLD
                     LLFYGQRVIDQPGLKVPHPRLHMRRFVLMPLSELAPLLVHPTRLRSVNQMLSETTDPS
                     EVRLLFPQPSTRYGSRPTCSPRMDS"
     gene            complement(2851..4026)
                     /locus_tag="VFR82_07400"
     CDS             complement(2851..4026)
                     /locus_tag="VFR82_07400"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEU4501460.1"
                     /translation="MAGFPIEVATRIKTLPPYLFAAIDKMKQAAIARGVDIINLGIGD
                     PDLPTPETIVESLAQAAKNPKHHQYPSYEGMLSFRQAVAEWYRRRFGVKLDPADEVLT
                     LIGSKEGIGHIHLAFVNPGDVVLVPSPGYPVYPVGTSFSGGVSHLMPLTKANGFLPEL
                     EAIPKDVAKQAKLMWLNSPNNPTSVVMTKDYFKRVVEFAHRHQIIVCHDAAYSEIFYD
                     GKRPASFMEVDGAKEVGVEFHSLSKTYNMTGWRLGFVVGNKDVLAGLGKVKSNLDSGV
                     FEAVQAAGITALKLDDAVTDAIRRIYQERRDTLVPGLKTVGLDVDTPPAAFYIWVAVP
                     KGYSSASFTEHLLEKAGIVTTPGNGFGAPGEGYVRMTVCTSKERLGEAVERIKKAGF"
     gene            4257..4643
                     /locus_tag="VFR82_07405"
     CDS             4257..4643
                     /locus_tag="VFR82_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501461.1"
                     /translation="MWHRIMLTPIWCAIWLMSAAYTGCVAESKDAAKRDVAGPYQSWD
                     EVITRWVGGQVSDLYVELGPPNLHPHQREDGTIEMTWDFAIDRMPGQADAFNLLPLYG
                     GNVNCQIHFFADLTGKVLKGERVGCE"
     gene            4743..5576
                     /locus_tag="VFR82_07410"
     CDS             4743..5576
                     /locus_tag="VFR82_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501462.1"
                     /translation="MQFTIKDVEDLRWLLGHTGGFRGGYVTNVQMAKRRLFDEGSGHE
                     VLADTVVTVVVRYHLRQMERVAKLTMKGVTDFSIFEQEGADCSSLGVIHAECTAGKLR
                     FWFDPQGELYVVCDEAQLDEVAMPSSDDCSREPVARWTFQSPTPEWPTIEWLLEELDG
                     AGLPCTWKKAKTPNEGRSVIQWEGTLLPAQGADTIEEAAVHVMLYGPLDGSGFGILLH
                     VDGSHVSAANRILSCLSDVIVSRFSGQCLVGNTIIPGGEWLNWKSLEQLRWRRAGKLE
                     E"
     gene            complement(5630..6361)
                     /locus_tag="VFR82_07415"
     CDS             complement(5630..6361)
                     /locus_tag="VFR82_07415"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4501463.1"
                     /translation="MDLHKVERVYTSYAGIYDKIFGKVFHEGRESAIRNLNVQPQERI
                     LEVGVGTGIALPMYPRHCEIVAIDFSEGMLEIAKKRTAEHQMDHVTLHRMDAGAMEFE
                     DSTFDTVVAAYVVTAVPDYRKVVSEMIRVCRPGGRIIMLNHFSNENKMVAAVEKVLSP
                     LTKHLGWRTDLSLDNVLEGTELQVARKQNVNPLRFWALVECVNCKDSEGVAHKKSAAY
                     ANGTTVYTPTPSSLSAGATNEEALA"
     gene            6637..7257
                     /locus_tag="VFR82_07420"
     CDS             6637..7257
                     /locus_tag="VFR82_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501464.1"
                     /translation="MWLAGLFACVLLIGLILGDWLYFVRLTPDAGRYGCGIARSFDRL
                     SVNSLARLQAQSDVNGALTLPHGIARYFPDLQQIALRPQYHLFSTSFRTAWPIKGLIH
                     LQLGEEVVHAVCIKRIPWSSAIVTLVWFLLVALGTTGFMIAYAAQGGFDSLSGVLMGI
                     GILGIGLLVLAFGVVTVMFSYRLESSRLAKVYEELKAMVNDTTGID"
     gene            complement(7303..7647)
                     /locus_tag="VFR82_07425"
     CDS             complement(7303..7647)
                     /locus_tag="VFR82_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501465.1"
                     /translation="MSHPASSQPDITQTLREEFRFHLETFYAQLKLAPPYESVEKAIR
                     ALTTALHALPKPQQSRIASDPVLKWDQFRQAFETSGLSRKHRGIITGLAKDQTSRTLP
                     PEYDHFLKLFQS"
     gene            7730..8194
                     /locus_tag="VFR82_07430"
     CDS             7730..8194
                     /locus_tag="VFR82_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501466.1"
                     /translation="MKRKWVALALFFAVGGCSSPHHHGMMGDKGDRYWQGQQELASLI
                     DQTVKDPVKAQEVKKLAGEIVTELKTGREQERAYHRQLYTLNTSYTATPEDFMKIIDE
                     GNHQRMRTAAKILNLRFKMKEQMTADEWQALSDRMRAYSGRYQHGGEPQAGY"
     gene            complement(8219..9256)
                     /locus_tag="VFR82_07435"
     CDS             complement(8219..9256)
                     /locus_tag="VFR82_07435"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501467.1"
                     /translation="MWSARVWQFAYPALIGLTLLSVGCQKDSEAIVSIALHPTNSKIL
                     YVATNDAVYKTRDGGQTWEKFPSFSARRVTTVAIDPQLPATVYAGTMGDAVYKSPDGG
                     QHWLPHNVGLKEHVSFVNQFVFHPTLSERIYAATTVGAFYTKDAGREWEERMNGMKEV
                     HIVTSVAIDPRDPSVLYGGTTGGIYRSEDGAMTWKKINTGLIPDSELMASMALGVNAI
                     EIDRTNPRVVYAGTTKGLYRTANRGEEWHRIGQSIPEHFVSSIVIHPSDSGVLYIGGP
                     AGVWKSADSGHTWQAINEGLGSLNIRALAMEPKNARTLYAGTNGSGLYRSVDAGATWT
                     AMPLRAAAPPS"
BASE COUNT         2026 a         2656 c         2780 g         2112 t
ORIGIN      
        1 aggacgggtt tgcgcaaggt gtcgtcctcg ccacgcccac caccctgatg gcgctcctca
       61 gagccgtagc ctacggctgg cgacaggagc ggttgactgc tcacgcggag gaggccggtc
      121 gcttgggaaa ggatctctat gaacgcatgg ccctgctcgc cgaatatgtg aacgacgtgg
      181 gactgtcgct cggcaagagc gtgctagcct ataacaaggc ggtcggctcc ttggagagca
      241 gaattcttcc cgcggcacgc cggttcaaag atttgggcgt gtcgtccgac aaagacattt
      301 catccttgag tcctatagaa gtcattccgc gaaaggcctt gtcgtatgag caagaatgag
      361 caaacagccc caccgacggc ccaatcgatg gtcgaagagt tccatcgttt gttcagcatc
      421 gtggtccatc agacgccgac ggtcatcgat gagcagaccc gtatcttgcg agaacgcctc
      481 atccatgagg aatttgagga actgaaagag gccatggcca agaacgactt gttcgcggtt
      541 gcgaaggagc ttgcggatct cttgtatgtc gtctatggga ccgccgtatc gtatggtatc
      601 gacatggagc cggtctttcg cgaagtgcac cggtccaata tgagcaaagt cggaggctat
      661 aaacgcgagg acggtaaatg ggtcaagccg gcaacctact cgcccgctat gatcgaaccg
      721 atattgacag aacaacaagc gcaacccggg agggaacaaa cttcccagcc gctggcggag
      781 atgaagtcgg gagcatgaag actctccatt gtccgaactg cggaacgccg ttcgtccgcg
      841 tcgcacctca atcgggggtg gtggaacgtg tgctgcacca cctcaatgtg gttccgttcc
      901 gctgccaact ctgcatgaac aagtttcatg ccttcagccg cgaggcgacc cgcagtgctc
      961 aagcgtccga tcgccggcag tacaaacggc ttcccacctc tatccaggcg cagttcctgg
     1021 cagacaatcg gatacggtcg accaatcgga ttacggacat ttcgatggat ggctgcaccc
     1081 tccagacgac cggcctgccg cggggaacgt tcctgggtct cgtgctcaag cccgaacagg
     1141 aagacgaggc gatccgaatt gaaacggcga tggtctgttc cgtgcgtccg acgtctgtag
     1201 ggctgcgctt tctcgagatt ccgttgcctg atcagcggcg cctcagccaa gtggtcctgg
     1261 gcttgcttgt gagccaaggc caccatccgg tctccaacgc ttagactgac aggccgccag
     1321 cgacgttctc ccttaactca cgggccgacg ttccgcaaca ccggatctct ctctcagctc
     1381 gatgccaacg tggtttagcc tccggtgagc agcgaggcgc ttccgatgct gtcatgaacc
     1441 attctttctc ctcgcaggca cgttgtcgat gaggcgtaca gacccaaggc gaatcgcacc
     1501 gaggatgatc gcctggcgct cgatgtggat gagaggctcc agcgtcgttg gatcgcaaac
     1561 agccaaataa tcgatccgaa tggcggactc ccctccgatc accttccgca tcagtgcctc
     1621 cactgacgcg gcatcgtgga ggccgttctc aattgctttg cggccggctt gtaaggtgtg
     1681 gaaaaagatc ggcgcaatgc ggcgatcttc cgttgacaga aagacgttgc gggaactcat
     1741 cgccaggccg tctcgttccc tcacagtagg gcagacgaca acgcgggtag gaaaattcaa
     1801 atcctttacc aattgccgta cgaccccaca ctgctgatag tctttttgtc cgaagagcgc
     1861 gatgccgggg cggacgacgt tgaaaagctt ggccacgacg gtcgctaccc cggtaaaatg
     1921 atggggccgg gattcccctt cccaccggcg tgcgatggac ggaaccgtca caagggtctg
     1981 aaaacctttg ggatacatgg cgtcagctgc aggctcgaaa cagacatcga cgccttcgtg
     2041 ccggcataac gctcgatctt tctcgattgg tctgggatag gtcgccaaat cttcggttgg
     2101 agcaaactgt gtcgggttca caaaaatact gacgacgagc gcgtcacact gccgtcttcc
     2161 ggcgcggatc aatgcccgat gcccgtcgtg aagcgcgccc atcgttggca ccaggccaat
     2221 gacgacccct tctcgatgta gctgattgct ccatgccgtc atcgccgaag gcgatcggat
     2281 cgtcttcatg aatccatgcg ggggctgcag gtcgggcgtg aaccgtagcg ggtcgacggt
     2341 tgtggaaaga ggagacggac ttcggatgga tcggtggtct ccgagagcat ctgattcacc
     2401 gaacgaagac gtgtaggatg tacgaggagc ggtgccagtt cgctcaaggg catcagaaca
     2461 aagcgtcgca tgtggaggcg tggatgggga accttcaagc cgggctggtc aatgacgcgc
     2521 tgcccataaa acagcagatc aagatcgatc gtccgtgggc ccgaacggtt gtcgtcgtcc
     2581 cggccaaggg aacgctcgat ttccctcaac atgctcaaaa ggctctccgg cgtgatggct
     2641 gtctctagtt gtacgacgcc attgaggaac catccaacgc cggggttggc tccgtctttc
     2701 accggctccg tctcgtagag cagcgaaacg ccggtaacct gcgagtgtgg caacagactc
     2761 aagagggtca gggcacgatc acaaaaatca atccgatcgc cgacgttgga gccaaaacca
     2821 atataggccg tctcgcattc catcctggcg ttaaaacccc gctttcttta ttcgctcgac
     2881 cgcttcaccc agccgctctt tcgaggtaca gacggtcatt cgaacgtacc cttcacccgg
     2941 cgcaccaaat ccgttaccgg gagtggtcac gattccggcc ttctccagta aatgttccgt
     3001 aaacgatgcg gagctataac ccttcggaac cgccacccag atataaaatg cggcgggggg
     3061 tgtgtcgaca tcaagtccca ccgtcttcaa tccgggaacc agcgtatccc tccgttcctg
     3121 gtagatgcgc ctgatggcgt cagtgaccgc atcatcaagt ttcaacgccg tgatacccgc
     3181 cgcttgcacg gcctcgaata cgcctgaatc caggttgctt ttcactttgc cgagaccagc
     3241 gagcacgtcc ttgttcccca cgacgaatcc cagtcgccac ccggtcatat tgtatgtctt
     3301 tgacagtgaa tgaaactcca ctccgacttc cttggcgccg tcgacttcca tgaaactggc
     3361 cggccgcttg ccgtcataga aaatctcgga gtaggccgca tcatggcaca cgataatctg
     3421 gtgccgatgc gcaaattcga cgacgcgctt gaaatagtcc ttggtcatga ccaccgacgt
     3481 gggattgttc ggggaattga gccacatgag cttggcctgt tttgccacgt ccttagggat
     3541 cgcctccagc tctggcagga acccgttggc cttcgtgagc ggcatgagat gcgagactcc
     3601 gccgctgaaa ctggtgccga ccggatagac gggatagcct ggactcggga ccagcaccac
     3661 gtcccccgga ttcacaaagg ccaaatggat gtgtccgatg ccttccttcg aaccgatgag
     3721 ggtcagcact tcgtctgccg gatccaactt cacgccaaac cggcgccgat accactcggc
     3781 caccgcttgg cggaatgaca gcatcccctc atatgaggga tactgatggt gtttgggatt
     3841 cttggcggct tgtgcgaggc tctcgactat tgtctcgggc gtcggcaggt ccggatcgcc
     3901 gatgcccagg ttgatgatat ccactccacg ggcgatcgcc gcctgcttca ttttgtcaat
     3961 ggcagcgaac aaataggggg gcaatgtctt gatgcgcgtt gcgacttcga tgggaaaacc
     4021 ggccatgatc gagggatctc ctttcgctca agaatgctgg cttcggcacc aagcctctag
     4081 gctacttcag gaataagccg gtaatcaatg taagagacta gctccggttc ttaacgagcg
     4141 tcttctgtgg cctgagtgaa gcgaatcctt tgaattgact cgttatccgc tgcccaatta
     4201 tgttgcgcca ggattccggt ctgcttcgcg gtgcatctct accgaaaggt ttggctgtgt
     4261 ggcatcgaat catgttgacg ccgatctggt gtgctatctg gctaatgagc gctgcctata
     4321 ccggctgcgt ggcggaaagc aaagacgcag cgaagcgcga tgtggccggc ccctatcaga
     4381 gctgggatga ggtcatcacc cggtgggttg gagggcaggt gtcggacctc tacgtcgaac
     4441 ttggtccgcc caatctacat ccgcatcaaa gagaagacgg gaccatcgag atgacgtggg
     4501 attttgcgat cgatcgcatg ccaggtcaag ccgacgcgtt caatctcctg cctctgtacg
     4561 gtggcaatgt gaactgccag atccattttt tcgccgatct gacgggaaaa gttctcaagg
     4621 gagaacgtgt gggctgcgag tgacaggcgc tccatccggg gctgtttctc attaagtggt
     4681 gggtgtctga cgaccggcga tcgttctggc ctccggcgtc atcctgcgtt acaatcgtga
     4741 ctatgcaatt cacgattaag gatgtggagg atctccgttg gctactgggc cacacaggtg
     4801 gcttccgcgg cggatatgtt acgaacgtgc agatggcgaa acgccggttg ttcgatgaag
     4861 gatccggcca cgaagttctg gctgataccg ttgtgacggt cgtggtgcgg taccatcttc
     4921 gtcaaatgga gcgcgtggca aaactgacca tgaaaggtgt cacggatttt tctatttttg
     4981 aacaggaagg cgctgactgt tccagcttag gcgtgatcca tgccgagtgt acggccggca
     5041 agcttcgatt ctggttcgat ccacagggcg agctgtatgt cgtctgtgac gaggcacagt
     5101 tggacgaagt ggcgatgccg tcgtctgacg attgctcgcg cgagccggtt gcccgctgga
     5161 ccttccaaag cccgactccc gaatggccaa caatagagtg gctgctagaa gagctggacg
     5221 gagcgggttt gccatgcaca tggaagaaag ccaaaacacc gaatgaagga aggtcggtca
     5281 tccaatggga aggcacgctg cttccggcgc aaggcgcgga caccatcgaa gaggccgccg
     5341 tgcatgtcat gctgtatgga ccgctcgatg ggtccggctt cggaatcctg ctgcatgtag
     5401 acggctccca cgtctcagcc gcaaaccgca tcctgtcctg cctgtctgat gtcattgtca
     5461 gtcgattttc gggtcaatgc ttggtcggca acaccatcat ccccggtggg gaatggttga
     5521 attggaaatc gttggagcag ttacggtgga ggagagcagg gaagctcgaa gaataatcag
     5581 agtgccgttg ccggcaccgc acacatggac caagagagtg ttattgagat caggcgaggg
     5641 cttcttcgtt cgtggctcca gcgctgaggc tgctaggagt cggagtgtag acggtcgtgc
     5701 cgttcgcgta ggcggcggat tttttgtgcg ccacgccctc gctgtcctta caattcacgc
     5761 attcaactag agcccaaaat cgtagcggat tcacattctg cttccgggca acctgcaact
     5821 cggttccttc cagcacattg tccaacgaca ggtccgtgcg ccagcccaga tgcttggtca
     5881 gtggcgacag cactttttct actgcagcaa ccattttatt ctcattgctg aaatgattga
     5941 gcatgatgat gcggccgccg ggtcggcaga cccgaatcat ctcgctgacg actttgcgat
     6001 aatcgggcac cgctgtcacc acgtacgccg cgacgaccgt atcaaatgtg ctgtcttcga
     6061 attccattgc gccggcgtcc atgcggtgca gcgtcacatg gtccatttga tgctctgccg
     6121 ttcgcttttt tgcgatttcc agcatgcctt ccgaaaaatc gatcgcgacg atttcgcaat
     6181 gacgcgggta catggggagg gcgatgccgg tgccgacgcc gacttccaag atgcgttctt
     6241 gcggttggac gttcaagttg cggatagcgg actcacgccc ttcgtgaaag accttgccga
     6301 agatcttgtc gtagataccg gcataggacg tgtagacgcg ctcgacctta tgcaaatcca
     6361 tatgacctcc aaccgcacgt gagacttctg cttctgcccg acagcaggga tgaaccgaag
     6421 atcaattcca atggggagaa tccgtaccgg cggagcgcct aaatggagcg aggaacccgc
     6481 gcctaacctg acacaagggc tacaataacc aagtcccgat atcgagtcaa tacaatagat
     6541 ttggacctag ggaaatccta gagggccaca gcgcaggaaa ctgccaagag tgttcccagc
     6601 ttgattcgag gcttcggcta agcatagggt acgaccatgt ggctggccgg tctcttcgca
     6661 tgcgtgctgc tcatcggttt gattctcgga gattggctct acttcgtacg cttgaccccg
     6721 gatgccggac ggtatggttg cggtattgca cgcagtttcg accgattgtc cgtgaacagc
     6781 cttgcacgcc ttcaggcgca gtccgatgtt aacggcgcgc tgacgctgcc ccatggcatt
     6841 gcccggtatt tcccggatct tcaacaaatc gcgctgcgcc cgcaatatca cttgttctcg
     6901 acgagtttcc ggaccgcctg gccaatcaag ggtctgattc atttgcagtt gggcgaggag
     6961 gtggtccatg ccgtctgcat caagcgaatt ccctggtcct ccgccatcgt caccctggtc
     7021 tggttccttt tagtcgcttt gggaacgacg ggtttcatga tcgcctacgc ggcacaaggt
     7081 gggttcgatt ctttgagcgg tgtgctgatg ggcatcggga ttctcggaat cggcttgctc
     7141 gtgttggcct ttggtgtggt cacggtcatg ttctcctacc ggctggaaag cagccggctg
     7201 gcgaaggtat acgaggaact gaaagccatg gtgaatgaca cgacaggaat agactgaatg
     7261 actcactcta tgcccgggtg gtaaccgcac agggaccggt ggtcaggact gaaagagctt
     7321 caggaagtgg tcgtattccg gggggagagt tctggaggtt tggtctttgg ccaggccggt
     7381 gatgatcccg cgatgttttc tgctcagccc cgaggtttcg aacgcctgac ggaattggtc
     7441 ccatttcaga acggggtctg aggcaatgcg ggactgctgc ggtttcggca gcgcatgcaa
     7501 agctgtggtc aatgcgcgga tcgctttctc gacgctttcg tagggagggg ccaatttgag
     7561 ctgggcgtaa aaggtttcca aatgaaaccg gaattcctcg cggagcgtct gagtgatatc
     7621 cggctgggat gaagcggggt gactcatcgc acgatcgatg atacgggaag atgattcccg
     7681 ctgacaatgg ggcctagcgg gattgatgtg gtgtagcaag gaggttcgca tgaaaagaaa
     7741 gtgggtggcg ttggcactgt tctttgccgt cggaggatgc tcctcccccc atcaccatgg
     7801 gatgatgggc gacaaaggag atcggtattg gcaagggcag caggagttgg ccagcctgat
     7861 tgaccaaacg gtgaaagatc ctgtgaaggc tcaagaagtc aagaaactcg cgggtgagat
     7921 cgtcacagaa ctgaaaacgg gccgtgaaca ggaacgcgcg taccaccgtc agctgtatac
     7981 actcaacacc agttatactg ccacgcctga agatttcatg aagattatcg acgagggaaa
     8041 tcatcaacgg atgcggactg cggcaaaaat tttgaacctg cgttttaaga tgaaggaaca
     8101 gatgactgcc gatgagtggc aggctctttc cgaccgcatg cgggcctaca gcggtagata
     8161 ccagcatgga ggcgaaccac aagcgggcta ttagagagga aagggatcac atctcaggct
     8221 agcttggcgg agcggcggct cggagaggca tggccgtcca tgttgcgccg gcgtcaaccg
     8281 agcggtagag cccgcttccg ttggtcccgg catagagtgt cctggcgttt ttcggttcca
     8341 tcgcgagcgc tcggatgttt aacgaaccga gtccctcgtt gatggcctgc catgtatgtc
     8401 cgctgtcggc gctcttccag acccctgccg gccctccgat atacaagacc ccagaatcgg
     8461 aaggatggat cacgatactg ctcacgaagt gttctggaat cgattgcccg attcggtgcc
     8521 actcttctcc cctgttcgcc gtccggtaga gacccttggt tgtgccggcg tacaccacgc
     8581 ggggatttgt cctatcgatc tcaatggcgt tgacgcccaa agccatggag gccatcaatt
     8641 cgctatccgg aatgagaccg gtgttgattt ttttccaggt catcgctccg tcctctgacc
     8701 ggtatattcc tcctgtcgtt ccaccgtaca gcacggatgg atctcttggg tcaatggcga
     8761 ccgacgtgac aatgtggact tccttcatgc cattcatccg ctcttcccat tcacgcccgg
     8821 catctttggt gtagaaagct cccacggtgg tcgcggcata aatccgttcg ctcagtgttg
     8881 gatggaaaac aaattgattc acaaaggaca catgctcctt caaccccacg ttatgcggca
     8941 gccaatgctg ccctccgtcg ggactcttat agacagcatc tcccatggtg cctgcgtaca
     9001 ccgtggcggg tagctgcgga tcgatggcca cggtcgtcac ccgtctggcg ctgaagctgg
     9061 gaaatttttc ccaggtttgg ccgccgtcgc gcgttttgta caccgcgtcg ttggtcgcga
     9121 catagaggat cttcgaattc gtgggatgca gagcgatgga gacaatcgct tcgctgtctt
     9181 tttgacagcc gacgctgaga agcgtcagcc cgatcagcgc ggggtaggcg aactgccata
     9241 cacgtgcact ccacatctgc cgaccctttt tgccctttct tccctcagtt tggggaggtg
     9301 tctcacatca ccactcgcgc tgagatgcgg gtaccacact tgtcaatcag atgcgaccaa
     9361 ccagcttggc gatgccgatg tttgaagttc cgttcatgcg aagcgcggaa cagaacaagt
     9421 ttcggcatcg agaagatgaa agacttagtg tgcctgcggc gaagcgccgg agcggctggg
     9481 tacctccgcg agtaactcta caatttcttc gagaagcgct ccgcataacc ggtcagctcc
     9541 atgtagccgc ggcccgcgat cggctttcct ccca
//
LOCUS       DASTQY010000227         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1136202, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000227 DASTQY010000000
VERSION     DASTQY010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136202"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFR82_07440"
     CDS             complement(<1..437)
                     /locus_tag="VFR82_07440"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HEU4501468.1"
                     /translation="MCAIAGILKLSRRERAEPSRLGRMSAVQQHRGPDGSGVWLDGPV
                     GLAHRRLAIIDVDGGHQPMTNEDDSLWIVFNGEIYNHAVLRLEMERAGHRYRTRSDTE
                     TILHLYETYGPRCVDRLQGMFAFAVWDRRERRLLLARDRLGIKP"
     gene            complement(440..1543)
                     /locus_tag="VFR82_07445"
     CDS             complement(440..1543)
                     /locus_tag="VFR82_07445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4501469.1"
                     /translation="MIKTQRPPNIAMVAPTFGSVAGQAVQAKVLADHLRLDRYTVEVV
                     PINPPFPRGARWLKRLRYVRTVANEGLYLPTLRKLRRADVVHVTAASYWSFLLAPLPA
                     IVAAHRWGKPILLHYSSGEADDHLANWGSLVHPWLKMVDAIVASSVFLRDVFARHGYE
                     AQVIHNVIDTGQFRYRRRDPLLPEFLSVRNFEPHHGVENTLVAFALIQTACPDASLTM
                     VGQGSQEAELKHLGQALALRNVRFLGSVEPASMPALYDAHSIFLNSSFVDNQPLSVLE
                     SMASGMPVVSTGVGDIPNMIEDGESGTLVPAADPSAMAKAAMLLLEQPDRALLMAQRA
                     KESLVQYDWSTVGQAWAETYRRLATRVSKREVA"
     gene            complement(1566..>2459)
                     /locus_tag="VFR82_07450"
     CDS             complement(1566..>2459)
                     /locus_tag="VFR82_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HEU4501470.1"
                     /translation="MAAQHVKVVLSGDGGDELFAGYTEYAVERRERWARFLPGVARQA
                     LGMISEAMPDGVRGKNWLRHFSLSDAERYLDANTMFKRADRYRLLTKETQAQLLDCQT
                     ERSRLERLTNGVGHWLSTLQSLDLDHYLPLDILTKVDRMSMAHSLEARVPLLDHSFVE
                     FAATIPPSWKMRRGRTKHIFVKALRGWLPDEILDRPKQGFAVPLDRWFRGPLRPLFRE
                     ILLSDTCRRRGIFDEQFLHRLFARQEQGRQLDPHIWTLMSFELWCRTFLDGPRTPCIR
                     PASHSTVIAASRMGRPLATSS"
BASE COUNT          523 a          744 c          719 g          473 t
ORIGIN      
        1 ggcttgatcc cgagccgatc gcgagcgagc agcaaccggc gctcgcggcg atcccagaca
       61 gcaaacgcga acataccttg caaccggtcg acgcaacggg gtccgtaggt ttcgtacaga
      121 tgaagaatcg tctcggtatc gctccgggtc cggtaacgat ggcccgctcg ttccatttcc
      181 agacggagca ccgcgtggtt gtagatttca ccgttgaaga caatccacag gctgtcgtct
      241 tcattggtca tcggctgatg tccgccgtcg acatcgatga tggccaggcg acgatgcgcc
      301 agacctaccg gcccgtccag ccacaccccc gatccgtccg gaccacggtg ctgctgcacc
      361 gccgacatgc gtcctaggcg cgatggctct gcccgctctc ggcgactgag tttcaagatt
      421 ccggctatgg cgcacatcgt caggccacct cccgtttgga gactcttgtt gcgaggcgac
      481 gatacgtctc agcccatgcc tgccccacgg ttgaccaatc gtattgcaca agggactcct
      541 ttgcgcgctg cgccatgagc agtgcgcgat ccggttgctc cagcagcaac atcgcggcct
      601 tcgccatcgc ggatggatcg gcggcaggga ccagtgtccc ggactcacca tcctcaatca
      661 tgttgggaat gtcgccgact cctgtgctca ccacgggcat acccgatgcc atcgactcca
      721 gcaccgacaa cggctggttg tccacgaacg aagagttcag gaatatcgaa tgggcatcat
      781 acagggccgg cattgaggcc ggctccacag agccgaggaa acgcacgttt cgaagagcga
      841 gtgcttgacc gagatgtttg agctccgctt cctgcgaacc ttggccgacc atcgtcaagc
      901 tggcatcagg acaggcggtc tggatgaggg caaaggccac gagtgtattt tcgaccccgt
      961 gatggggttc gaaatttcgt accgacagaa attccggcag cagggggtcg cgccgccgat
     1021 accgaaactg tcccgtatcg atcacattgt gaatgacctg cgcctcgtag ccgtgacggg
     1081 caaacacgtc gcgcaagaac accgaagacg cgacgatggc gtcgaccatc ttcaaccacg
     1141 gatgcacgag agatccccaa ttggcaaggt gatcgtccgc ttcaccgctg gaatagtgta
     1201 agaggatcgg cttcccccat cgatgcgctg caacgattgc ggggagcggg gccaggagaa
     1261 acgaccaata ggatgcggcc gtgacatgca cgacatcggc acgtcgcagc tttcgcagag
     1321 tgggcagata cagtccttcg tttgccactg ttcggacgta ccgtaggcgc ttgagccagc
     1381 gcgctccgcg cggaaacggt gggttgatag gcaccacttc gacggtatat cggtccagtc
     1441 gtaaatgatc tgccaacacc ttggcttgaa cggcttgtcc agcgacactg ccgaaagtcg
     1501 gcgcaaccat ggcgatgtta ggaggccgct gggtcttgat catggtctgt gtttagtgag
     1561 tctgcctacg acgatgtcgc tagcggcctg cccatacgtg aggcggcgat cacggtcgaa
     1621 tgcgaggcgg gccgaatgca gggcgtgcgt ggcccatcca ggaacgtgcg gcaccagagc
     1681 tcaaacgaca tgagcgtcca gatgtgagga tccaattggc gcccctgttc ctgccgggcg
     1741 aataatcgat ggaggaactg ctcgtcgaag atcccccgcc gacgacaagt gtcagacaac
     1801 agaatctccc ggaatagcgg acgcagcggg ccacgaaacc accggtcgag cggtacagcg
     1861 aagccttgtt tgggacgatc gagaatttcg tcgggaagcc acccccgcaa ggccttgacg
     1921 aagatatgct tggtcctccc ccgacgcatt ttccaactgg gcggaatcgt ggccgcgaac
     1981 tccacaaagc tgtggtctag caacgggacg cgcgcctcca gggaatgggc catgctcatc
     2041 cgatcgactt ttgtcagaat gtcgagcggg agataatggt ccaaatccag cgattgtagg
     2101 gtagacagcc aatgccctac gccgtttgtg agccgctcga gacgtgatcg ttcggtttga
     2161 caatccagca gctgagcttg cgtctctttc gtcaacaggc ggtaccggtc ggctctcttg
     2221 aacatcgtat tggcatccag atatcgttcc gcgtcggaaa gggagaaatg acgcagccag
     2281 tttttccctc gtaccccgtc cggcatggct tcagaaatca tcccaagtgc ctgtcgggca
     2341 acaccgggaa gaaatcgtgc ccagcgttcc ctgcgctcaa cggcatattc ggtgtacccg
     2401 gcgaacaatt catcgcctcc atcaccggac agcaccacct tcacatgctg cgccgccat
//
LOCUS       DASTQY010000228        11259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1676822, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000228 DASTQY010000000
VERSION     DASTQY010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11259
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676822"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFR82_07455"
     CDS             complement(<1..450)
                     /locus_tag="VFR82_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU4501471.1"
                     /translation="MTKRLTNQRMAELGQSEIRAMTQACVAAGGLNMAQGVCDTPVPP
                     VVLKGAAEAMVQGRNSYSRFDGLPELRQALAVKLGRDNGIQADPETDITVSAGATGAF
                     HSACVALLNPGDEVILFEPYYQYHITALMAVEARPVIVPTTGPDWTVI"
     gene            742..1032
                     /locus_tag="VFR82_07460"
                     /pseudo
     CDS             742..1032
                     /locus_tag="VFR82_07460"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
     gene            complement(1029..1649)
                     /locus_tag="VFR82_07465"
     CDS             complement(1029..1649)
                     /locus_tag="VFR82_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU4501472.1"
                     /translation="MLTWLSGMTTCHTDMLRRVGQASCTVLMAMLIVLGVPTATNSDF
                     DSRQHYSQQEIRRAIVFYARRYRLDPALLRAVMKAESGYRQHVVSKKGAIGLMQLTPD
                     TAATLRVANVYDSMENIRGGAKQLRHLLNLYGSDLPLALAAYNAGVHRVKGRTVPRIR
                     ETRAYVRKVLRYYRALKGEHRLLRQVHPQPKPIGGEHQLVKSAGGR"
     gene            complement(1795..2421)
                     /gene="msrA"
                     /locus_tag="VFR82_07470"
     CDS             complement(1795..2421)
                     /gene="msrA"
                     /locus_tag="VFR82_07470"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU4501473.1"
                     /translation="MTALTWAMPFLLAVIGIVAGEQSSVREGQAAESGTMAKAYFAGG
                     CFWCMEEAFEKVDGVIDVLSGYMGGSVKNPSYEDVSAGRTGHAESVEVRYDPRKVTYS
                     QLLEVFWRNVDPVTPEAQFCDHGNQYRAAIFYQGEEEKRLAEESKRAIEQSKRFSTPI
                     ATQVTAASEFYPAEEYHQDFYKKNPFRYKYYKFTCGRAQRLEALWGKS"
     gene            complement(2512..3045)
                     /locus_tag="VFR82_07475"
     CDS             complement(2512..3045)
                     /locus_tag="VFR82_07475"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501474.1"
                     /translation="MRYVVGVMGPAKARRKDVENARVLGELIARREWVLLTGGRDVGV
                     MHAASQGAKKVARSLTVGVLPTTRDRISKYVDVAIVTEMGSARNNICVLSSQVIVACG
                     LGGSGTVSEVALALKAGKEVVLLGATPVEEKFFKNLGGRLVSSVQTPEDAINVILRRY
                     EQSDGDAPHPAQSWGHF"
     gene            complement(3171..4814)
                     /locus_tag="VFR82_07480"
     CDS             complement(3171..4814)
                     /locus_tag="VFR82_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501475.1"
                     /translation="MTSDREFEAGWLTRLAAALLGLLLSSPPSYAERPLYDNLGSLHH
                     PISTTSELAQRYFDQGLRLVYAFNHEEAIRAFEEAAKQDPNAPMPYWGVGLALGPNIN
                     AAMSKEEERRAIEAVKQARNRLAAATPAERAYIDALTKRYVSTRRASRASLDKAYADA
                     MRTVWRTYADDPDAGVLFAEALMDLRPWDFWTAEGKPKPGTEEIVSTLEAVIKTHPDH
                     PGACHYYIHAVEASRHPERALACADRLPGLMPGAGHLVHMPAHIYARVGKYHESAVRN
                     AEAAQVDEHYLTSARLTGDYADGYYSHNLHFLWSSLMMEGRSAEALQAAATLLSTITD
                     EEARSNRGKEAYLTTKLFALIRFGRWEELLREPPPSKGLHLVEGIWRLGRGLALVGTG
                     RFPGAEGERQALSTLTKQFRRSKNQQDKLERDLLKIAERLLAGQIAAQRQRYDEAIRA
                     LREAVSLDDSLQGAEPPHWPLPVRQYLGPVLLLSSRASEAEVEYRADLKWYPENGWSL
                     FGLLQSLRAQRKESEADEIAGRFEKAWTHADVTLAASRF"
     gene            complement(4825..6168)
                     /locus_tag="VFR82_07485"
     CDS             complement(4825..6168)
                     /locus_tag="VFR82_07485"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEU4501476.1"
                     /translation="MTIANVKIWPRMTGRDEFRFLADLVLKRSSADQTVVNLHDQHSG
                     TTRFASSRIIQNVDTRRGTLTVTVAFGGHRGSASTTDFTAGSVQDALARAERIARVSP
                     EDPEYLPPPDPQVFPTLQTSRPETAAAGPPRRLEYANEAIGQCRIENLEAAGIVSSSV
                     ASVGVAASNGLFAQEERTEARFSLTVQAEDATGWSAAAHRSIDHLKIQDRTLTAINKA
                     KRSRDVQELAPGRYRVILEPAAVAGLWSWMLWTLDAKSYEKGTSPFADQLGRQILDER
                     LTLQNVPGHPDLLGTGFTHEGLPSEEALWIEKGVLKQLVYDRFTAKQQRIKRIATLEA
                     PCLSASGPTAFKTQELIKQCERAILVTNFWYIRTVNPTDLTLTGMTRDGTFLVEDGQI
                     TSAVRNFRFHESPLRVFKHVDACTEPAEAVTSETGKMLVPAVILRDFNFSSVTRF"
     gene            complement(6176..7621)
                     /locus_tag="VFR82_07490"
     CDS             complement(6176..7621)
                     /locus_tag="VFR82_07490"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEU4501477.1"
                     /translation="MTLLDWDEMAELALKRVRESGAEYGDIRLMQSMSQTISGEDRRI
                     ASVRDVADSGFGVRVLYHGGWGFAASSILSIEEVPRVADLAIEIAKGSASLASERARL
                     VDEPIHRDRVVTGNRIDPFTVPLDQKTMLLQDVMEYVQRQPEVVRSHASLWARGDKKL
                     FVSTEGSRLEFDLLAVQGEFTATARHDGRFASRTFTTPHLRTGYELIESANFMAEAPR
                     IASQAVEKVKAPAVRPGAYDLVLDPEHLSLTMHESCGHPSELDRALGYEANYAGTSFL
                     TPEKRGSFHYGSSQVNLVADNTEPDTLAATGYDDDGVACQKWDIVREGVFVGYCTNRE
                     VATKIGEARSKGSNRADSWGSVPIVRIANIGLEPGQASLEDLLADVKRGVYIEGHGSY
                     SIDQRRYNFQFGGDAFWLIENGKRTHMLRDVIYHGITPEFWNSCDGVADRRYRRRYGF
                     ITCGKGQPGQSGWMTHAAPHARFRRVAVIKG"
     gene            7696..8001
                     /locus_tag="VFR82_07495"
     CDS             7696..8001
                     /locus_tag="VFR82_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501478.1"
                     /translation="MLPAPRTARQRMIELLSGTRLSAYQLAQMLGISERQVEDHLVHV
                     VRTIARDRERHFILEPSTCPECHFIFQDRTKLTRPSRCPRCRGESITAPRYGIDGPS"
     gene            8086..9672
                     /locus_tag="VFR82_07500"
     CDS             8086..9672
                     /locus_tag="VFR82_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEU4501479.1"
                     /translation="MNSILGIAAPSSFSALLGGLKHDGLASIVVFLVALPLCIGITIA
                     SGAPTGSGIVTGIVAGIVVGWLSGCPLQVSGPAAGLTVIVYELIREQGLEAFAVIVIA
                     AGFIQILWSWLQLGQWFRAVSPAVIHGMLAGIGALIFASQFHIMIDDLPKGSGLENIL
                     SLPAAVWKGLIDDDSTPINHHLAARIGVLTIGTMVLWNVVLPKRFHVMPAVLLGVATA
                     TAASALLDIPIQRIDVQDNLLSIVHFPTPSMLSHFFDTSILAEALGLAFIASVETLLS
                     ASAVDQLHTGPRTRYNRELMAQGVGNMICGFLGSLPMTGVIVRSSANVEAGARTRASA
                     ILHGVWLLLFVSLLPSVLRFIPTASLGAVLVFTGYKLMNFGILPELKKHGRGEVVIFF
                     VTMGTIVFVNLLTGVVLGLALALAKLLHTTQNLEAFYAHHPDSGKLTLHLQGIATFVS
                     LPRLAATLETAPPHAEIHLDFASLRHIDHACLNLLQNWQQQHEAHDGRVVADWDKMRG
                     ASYARRQTLRKTSWWQHWLE"
     gene            9731..9943
                     /locus_tag="VFR82_07505"
     CDS             9731..9943
                     /locus_tag="VFR82_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501480.1"
                     /translation="MTRLLTAILLILVMACTGDKAKDLLETAEFEERQMNIPHAKQLY
                     EEVIRLYPSSQEAEKARARLAKLSQT"
     gene            complement(9930..10736)
                     /locus_tag="VFR82_07510"
     CDS             complement(9930..10736)
                     /locus_tag="VFR82_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEU4501481.1"
                     /translation="MNSDPLFLVMVGYGAMVVLMLVLWGGQVVLRNASIADVGFCAGL
                     IVIVLWYTRYGFGEPERKLLVAFMVCVYAGRLGLFLLFNRVIGKAEDARYQEMRRSWG
                     ASEQAKMFGYFQLQAAAVAAFSLPFLVLIQNPRPPFGFVELAGFLMWIAAISGEAAAD
                     RQLARFRAQPWNRDRVCRDGLWYYSRHPNYFFEWLHWWSYVVMAIGAPGWPLTLIGPA
                     GMGWALLRVTGVPLAEQQALKTRGAEYRHYQQTTNAFVPWLPKRLPIKSD"
     gene            complement(10733..>11259)
                     /locus_tag="VFR82_07515"
     CDS             complement(10733..>11259)
                     /locus_tag="VFR82_07515"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEU4501482.1"
                     /translation="DAPAPINVYGATKLAAERIVLQNVRHSVVRIVLTAGTSLRRDRS
                     FVEDMCRAAKAGSSITLYADEFRCPLPAGVVARAIWELICSGRTGLYHLGGRERLSRW
                     EIGQLLLEWFPELQGRLVKGSSRDHTGAPRPPDLSLSTTKIQHVLSFPIPGMQSWLGE
                     RSCRGHDLWDYPLP"
BASE COUNT         2262 a         3440 c         3173 g         2384 t
ORIGIN      
        1 gatcaccgtc cagtcgggcc cggtcgttgg tacgataaca ggtcgcgctt ccaccgccat
       61 caaggcggtg atgtgatact gatagtacgg ctcgaacaga atgacttcgt ctcccggatt
      121 tagcagcgcc acacaagccg aatgaaaggc cccggtcgcc ccggcgctta ccgtgatgtc
      181 tgtttccgga tccgcctgta ttccgttgtc tcggcctaac ttgaccgcga gggcctgacg
      241 taactcgggc agtccatcga accgcgaata actgttgcgc ccttgcacca tagcctctgc
      301 ggcgcctttt aagacaaccg ggggcaccgg cgtatcgcac acgccctgag ccatattgag
      361 accaccggct gcgacgcaag cctgagtcat ggcccgaatc tcggattgtc ccagctcggc
      421 catccgttga tttgtcagtc tcttggtcac ttgatgctcc ctcccctttt gtcgcagagc
      481 tcattaaggc ccgtcaaggg ttggaatggt gccatacatt tcttacgtat ttactcgaat
      541 gttgcggggg catcatggta cagtcgcgac tagttcgttc gagttgattc tggcggggat
      601 tgcgagcaga gagcaatcct ctatactcag catgagagct agggaaacta ccagaggtga
      661 aagcatgggg taggtaaggg aggaccctct caaacggacc gaaagcctga cggtccgcag
      721 gacggtgaac ggcggggtcg ccgacttgaa atgaaccgcc gtgtgttctt cttcggagac
      781 agcgatttcg aaggtgaagc gaccttactg gatatctcga cgaacggatg cagggtgact
      841 tcgttgacgg aagtgcaaca gggacagtct ctcaagttgt ccatctttct tcaagacgtg
      901 gagtggccgt tacgcatcga tcatgcgatc gtccggtggg tggacggcca gtctttcggc
      961 atggaattca ccggcatcag gccggctcag cgtgaacgcc tacgatcgat tttgatgaag
     1021 gccaaggact aacgcccacc cgcactcttc acaagttgat gctctcctcc gatgggtttg
     1081 ggctgaggat gcacctggcg aagcaaccga tgctctccct ttagggctcg ataatacctg
     1141 agcactttcc tgacgtacgc acgcgtctcg cgtatccgcg gcaccgtgcg gcccttcacc
     1201 cgatgcactc ccgcattata ggcagcgagc gccagaggaa ggtcgcttcc gtataggttc
     1261 agaagatggc gaagttgttt ggcccctccg cggatgttct ccatcgaatc gtacacgttg
     1321 gcgacacgca acgtcgcagc ggtatcaggc gtcagctgca tcaatccaat tgcgcccttt
     1381 tttgaaacca cgtgctgacg atacccggac tcggccttca tcactgcacg cagcaacgcc
     1441 ggatcaaggc gatagcgtct ggcatagaag acgatcgcac gacggatctc ctgctgggaa
     1501 tagtgctggc ggctgtcgaa atcggaattt gtcgcggtgg gtacgccgag aacgatgagc
     1561 atcgccatca gtacagtgca ggaggcttgc ccgacgcgcc gcagcatatc ggtgtgacat
     1621 gtggtcatcc cgcttaacca ggtcaacatt catgccgcac tctcgtttat gtaaggctgc
     1681 gaaaaggtag ggagaaaccg ttggtgcctc ggtggtaaca acggaacaga actgttcaaa
     1741 gtacgacagg ctgcgtcatt tttgatgcag tatatgaaat gacgtggccc gtcgttacga
     1801 cttcccccat agcgcttcaa ggcgttgcgc ccggccgcag gtgaacttgt agtatttata
     1861 gcgaaaggga ttcttcttgt agaaatcctg atggtactcc tcggcaggat agaactcgga
     1921 agcagctgtc acttgcgtcg caatcggcgt cgaaaaccgc tttgattgtt cgatagcgcg
     1981 ttttgattcc tctgccaggc gcttctcctc ttcgccctga taaaaaatgg cggctcggta
     2041 ctgattccca tgatcgcaaa attgcgcctc cggtgtaacc ggatcgacat tgcgccagaa
     2101 gacttcgagc aattgtgaat acgtgacttt tctcggatca tagcgcactt cgaccgattc
     2161 agcgtgacct gttcgtcccg ccgatacgtc ttcatagctc ggattcttga cgcttcctcc
     2221 catataaccg gacagcacgt cgatgacccc gtccaccttt tcgaaggctt cctccatgca
     2281 ccagaagcac ccgcctgcaa aataggcttt tgccatagtg ccggattcgg ccgcctgccc
     2341 ttcacggaca ctcgactgtt cgcctgctac gatcccgatg actgctaata ggaaaggcat
     2401 tgcccacgtg agagccgtca tggttatcct catttcgcgt gcccagatcg cgatgctccg
     2461 gtcaatcaat ctacggccga tgcttcgcct cggatgcatc tgttcaacca gctaaaaatg
     2521 cccccaggat tgtgccggat gcggagcgtc tccatcgctc tgttcatacc gccggagaat
     2581 gacattaatg gcatcctccg gagtttgtac actcgagacc agccggcccc cgagattttt
     2641 gaagaatttt tcctcgaccg gcgtcgcccc aagcagtacg acttcctttc ccgccttgag
     2701 tgccagagca acctcggaaa cggtccctga tccacccaac ccgcaggcta caatgacctg
     2761 acttgacaac acgcagatgt tgtttctcgc gctgcccatt tccgtgacga tcgccacatc
     2821 cacgtacttg gaaatcctat cccgtgtcgt gggcaagaca cccaccgtca agctcctggc
     2881 cacttttttt gccccctgac tggcggcgtg catgacgccc acatcgcggc cgcccgtcag
     2941 cagcacccac tcgcgccgag caatgagttc cccgagcact cgtgcattct ccacatcttt
     3001 tcttctcgcc ttggcaggcc ccatgacgcc cacgacatat cgcacatgcc cctcctgttc
     3061 ggaatgaggc gattgtagcc ggtttgagtc caggcttgtc aaaagagtac cgctcggtgg
     3121 aaaacgcgtg gtctgaacgg ggggcccggt acgatatacg tcacgtcctg ttagaatcgg
     3181 gacgcagcga gtgtgacatc ggcatgcgtc caggccttct cgaaccgccc ggcgatctca
     3241 tcagcctccg actccttccg ttgggccctt agactctgaa ggaggccgaa cagactccat
     3301 ccgttttcgg ggtaccattt caaatccgcc ctgtattcca cttcggcttc ggaagcacga
     3361 ctcgacaaca agaggacggg accgagatac tgccgcacag gaagcggcca atgcggcggt
     3421 tccgcaccct gcaacgagtc atcaaggctg actgcctccc tcaatgccct gatcgcttcg
     3481 tcgtagcgct gccgctgggc ggcaatttgc ccggcgagca gccgttcagc gattttcagt
     3541 aggtctctct cgagcttgtc ctgttggttc ttgctccgtc ggaactgctt cgtcagcgtg
     3601 ctgagcgcct gtcgctcccc ttctgcgccc gggaatcgcc cggtgccgac tagggccagg
     3661 ccacgtccca accgccagat cccttcgaca aggtgcaggc ctttcgatgg cggcggctcg
     3721 cgtaagagct cttcccagcg tccgaaacgg atcaaggcaa agagtttcgt tgtcagatag
     3781 gcctctttgc cgcggttgct tcgagcttcc tcatccgtga tcgtggagag aagcgtggcg
     3841 gcggcctgca aggcctccgc gcttcgtccc tccatcatga gcgaggacca caaaaaatgc
     3901 aggttgtgac tgtagtagcc gtccgcataa tcgccggtca gacgagcact cgtgaggtag
     3961 tgttcatcga cctgcgccgc ctcggcattt ctgaccgccg attcgtgata cttcccgacg
     4021 cgggcataaa tatgcgccgg catatggaca aggtggccag cacctggcat caagccgggc
     4081 aagcgatctg cgcaagccag cgcgcgctcc ggatgccgtg aagcttcgac ggcatggatg
     4141 tagtaatgac aggcgccagg atggtcaggg tgcgtcttga tcaccgcctc gagtgtcgac
     4201 acgatctctt ccgtccccgg cttgggtttt ccctccgccg tccaaaaatc ccagggccgc
     4261 aggtccatga gggcttcggc gaacaggaca ccggcgtccg gatcgtcggc ataagttcgc
     4321 cacaccgtgc gcatggcgtc cgcataggct ttatcgagag acgcgcgcga cgcgcggcgt
     4381 gtggacacat accgcttggt tagggcgtcg atgtaggccc gctcggccgg ggtggcggcg
     4441 gcaaggcggt tccgtgcttg tttcaccgct tcgatggccc tgcgctcctc ctctttggac
     4501 attgccgcat tgatgttcgg tcccagagcc aatccgacgc cccagtaggg catcggggcg
     4561 ttcggatcct gcttcgccgc ttcttcgaac gcgcgaatgg cttcttcgtg attgaatgca
     4621 tagaccagcc gcaagccttg atcaaaatac cgctgagcca gctcggacgt ggtactgatc
     4681 gggtgatgca gggagccgag attatcgtag aggggtctct cggcgtacga aggtgggctc
     4741 gacagtagaa gaccgagcag ggcagcggcc aaacgcgtga gccatcctgc ttcgaattct
     4801 cgatcactcg tcattcatgg ttcctcaaaa tctggtgacg cttgaaaaat tgaagtcccg
     4861 gaggatgacg gctggcacca acattttacc tgtttctgag gtgacggctt cagccggttc
     4921 ggtgcacgcg tccacgtgct tgaacacgcg caatgggctc tcgtgaaaac ggaagttgcg
     4981 cacggcggag gtaatctggc cgtcctcgac caagaatgtg ccgtctcgcg tcataccagt
     5041 cagcgtgagg tccgttggat tgacggttct gatgtaccag aaattcgtca caaggatcgc
     5101 acgctcgcac tgcttgatca gttcctgtgt cttgaacgcc gtcgggccgg acgcggacaa
     5161 gcacggcgct tccagcgtcg cgatgcgctt gattcgctgt tgtttggcgg tgaatcggtc
     5221 atacactaat tgcttcaaca cgcccttctc tatccacagc gcctcttcgc tcggcagtcc
     5281 ttcatgggtg aaccccgtac ccagcaggtc gggatgcccg gggacgtttt gtagcgtaag
     5341 ccgttcgtcc agaatctgac ggccgagctg gtctgcaaag ggactggtcc ccttctcata
     5401 ggatttggcg tccagcgtcc agagcatcca gctccacagt cccgccacgg cggccggctc
     5461 caagatgacc cggtaccgac caggggccag ttcttgaacg tcccgactcc gcttggcttt
     5521 gttgatcgcc gtcaaggtcc ggtcctggat cttcaaatga tcgatcgagc ggtgagctgc
     5581 cgcgctccag ccggtggcat cttctgcttg cacggtgaga ctgaaacgcg cctccgtacg
     5641 ctcttcctgt gcaaacaagc cgttgctggc ggcgacacct accgacgcga ctgaagaaga
     5701 gacaataccg gcggcttcaa gattttcaat acggcactgt ccgatggcct cgttcgcata
     5761 ttccaaacgt cgcggcggac ccgccgcggc cgtctccggc cggctcgttt gaagcgtcgg
     5821 aaagacttgc gggtccgggg gcggcagata ttccggatcc tcgggagaga cgcgggcaat
     5881 acgttcggcg cgggcgagcg catcttgcac ggatcctgca gtgaaatccg tcgtgcttgc
     5941 ggatccccga tggcctccga acgccacggt gacggtcaac gtcccccgcc tcgtatcgac
     6001 attctggatg atacggctgc ttgcgaagcg agttgtcccg ctatgctgat catgcagatt
     6061 gacgaccgtt tgatccgcag acgaacgctt gagcacaagg tcggccagaa atcggaattc
     6121 atcacgcccg gtcatcctgg gccagatttt gacattcgcg atggtcacga tcggttcatc
     6181 ctttgatgac ggccacacgg cgaaaccgcg catggggcgc agcgtgcgtc atccagcccg
     6241 attggccagg ctggcccttt ccacacgtga tgaagccata tcgccgacgg taccgccgat
     6301 cggcgacacc atcgcagctg ttccaaaact ccggggtgat gccgtgataa atgacgtccc
     6361 ggagcatgtg ggtgcgtttg ccgttttcaa tgagccagaa cgcatcgccc ccgaactgaa
     6421 agttatagcg gcgctggtca atgctgtaag acccatgtcc ttcaatgtag actccccgtt
     6481 tcacgtccgc gagcaggtct tcaagcgaag cctggccggg ctccaagccg atgttggcaa
     6541 tccgcacgat cggcacgctc ccccagctat cggcccgatt cgaacctttc gatcgggcct
     6601 cgccgatttt tgtggccacc tcgcggttcg tgcaatagcc gacgaatacg ccttcgcgga
     6661 caatgtccca cttctgacag gccacgccgt cgtcgtcgta accggtagca gccagcgtgt
     6721 ccggttctgt gttgtccgcg acgagattga cttgcgaaga cccgtagtga aatgagccac
     6781 gcttctcggg cgtgagaaag ctggtcccgg catagttcgc ctcatacccc aacgcccggt
     6841 cgagctcgct cggatggcca caggattcgt gcatagtcaa tgaaaggtgc tccggatcga
     6901 gcacgagatc gtacgcgccg ggtcgcacgg ccggcgcttt gaccttttcc accgcctgag
     6961 atgcgatacg cggagcctcg gccatgaaat ttgccgactc gatgagctcg taccccgtac
     7021 gcaagtgcgg cgtggtgaac gttcgcgagg cgaagcggcc atcgtgccgg gcggtcgccg
     7081 tgaattcccc ctgtactgcg agcaagtcaa attcgagacg tgagccctcg gtcgacacga
     7141 acagtttctt gtcgcccctt gcccacagac ttgcatggct gcgcacaact tcaggttgac
     7201 gctgcacgta ctccatcacg tcctgaagca gcatggtctt ttggtcgagc gggacggtga
     7261 agggatcgat ccggttgccg gtgaccactc gatcacggtg tatcggctca tccacaagtc
     7321 gcgccctctc gctggccaga gaggccgatc ctttggcgat ctcgatcgcc aaatcggcca
     7381 cgcgcggcac ttcctcgatg gacaaaatcg aactcgcggc aaatccccaa cccccgtgat
     7441 aaaggacgcg cacgccaaac ccgctgtcgg ccacatcgcg gaccgaagca atacgccgat
     7501 cttccccgct gatcgtttgg ctcatgctct gcatgaggcg gatgtctccg tattctgcgc
     7561 cggactccct cacgcgcttc aatgccagtt ccgccatttc atcccagtcc aaaagcgtca
     7621 tcgtgccttc atcctgccat tgtcacgtga gccattcaac ttggaattca ccgaattctt
     7681 ccctaggatg gcaggatgtt gccggcacca cgaacggcac gacagcgcat gatcgagctg
     7741 ttgagcggaa cgagactttc cgcctaccaa ctcgcgcaga tgctcggcat ctcggagcgg
     7801 caagtcgagg accatctcgt ccatgtggtg aggacgatcg cccgagatcg ggagaggcat
     7861 ttcattctgg aaccctccac ctgtccggag tgccacttca tttttcagga ccgcacgaaa
     7921 ctgacgagac caagccgctg tccgcgctgc cgtggcgaaa gcatcaccgc accccgctat
     7981 ggcatcgatg gaccctcctg agaggctact actgcggctt ttcagcattt gcttggggga
     8041 tcatccttcg tcgactagag tgagcctaca agcatcgaga cccctatgaa cagcatcctc
     8101 ggaatcgctg cgccatcctc attctccgcc ctgttaggcg gcctgaaaca cgatgggttg
     8161 gcctccatcg tggtcttctt ggtggccctg cccttgtgca tcgggatcac gatcgcgtca
     8221 ggggccccga ccggttcggg catcgtcacg ggtatcgtcg ccggcatcgt cgtcgggtgg
     8281 ctttccggct gtcccttgca ggtgagcgga cccgccgccg ggttgaccgt cattgtctat
     8341 gaactcattc gcgaacaggg gctggaagca ttcgcggtga tcgtgatcgc cgccggcttc
     8401 attcaaattc tctggtcgtg gctgcagttg gggcagtggt ttcgcgctgt ttcgccggcc
     8461 gttattcatg gcatgctggc gggcatcggc gcccttatct ttgccagtca atttcacatc
     8521 atgatcgacg acctgcccaa ggggtcaggg ctcgagaaca tcctttccct tccggcggcg
     8581 gtctggaaag gcctgattga cgacgacagc acgccgatta accatcatct cgcggcgcgt
     8641 attggcgtcc tgactatcgg tacgatggta ctgtggaacg tcgttcttcc caagcggttc
     8701 catgtcatgc cggccgtgct cctgggcgtg gccactgcca ccgccgcttc cgcactcctg
     8761 gatatcccga ttcagcgcat cgacgtacag gacaatctcc tatccatcgt gcactttccc
     8821 actcccagca tgttgtctca ttttttcgat acctcgattc tcgcggaggc actcggtctc
     8881 gccttcatcg cgagcgtgga aacgcttctc tccgcctcgg cggtagatca gctgcacacc
     8941 ggtccgcgga cccgatacaa ccgggaactg atggcacagg gcgtggggaa catgatttgt
     9001 ggtttcctcg gctcattgcc gatgacgggg gtcattgttc gcagctccgc caacgtggag
     9061 gccggcgccc gcacgagggc atcggcgatc ttgcacggcg tctggctact gctcttcgtc
     9121 tccttgctgc cgtcggttct ccggttcatt ccgacggcga gtctgggcgc tgtgctcgtc
     9181 tttacggggt ataagctcat gaacttcggg attctgccgg aactgaagaa gcatggccgg
     9241 ggcgaagtcg tcattttctt tgtcaccatg ggcacgatcg tcttcgtcaa tttactgacc
     9301 ggcgtcgtgc ttggcttggc cctcgccctt gcgaaactgc tgcacacgac ccagaatctg
     9361 gaagcgtttt atgcgcatca tcctgattca ggaaagctga ccctccacct gcagggaatc
     9421 gcgacattcg tcagtctccc ccgattggcc gccacgctgg agactgcgcc gccgcatgct
     9481 gaaatccacc tggacttcgc atcgctgcgg cacatcgatc acgcctgcct gaatctgctc
     9541 caaaactggc aacagcagca tgaggcgcac gatggcaggg tcgtcgcgga ctgggataaa
     9601 atgcgagggg cctcctatgc gaggaggcag actctccgga agacatcctg gtggcagcat
     9661 tggctcgagt agccgcacgg tgcttgtccc cggctgtgga ctcagtggcc cccccaacaa
     9721 tggagcacac atgacccgct tgctcaccgc catcttgctg attttggtca tggcctgcac
     9781 gggcgacaag gcgaaggacc tgttggaaac ggcagaattc gaggagcgcc agatgaatat
     9841 tcctcacgcc aagcagctgt atgaagaagt gattcgtctg tatccctcga gtcaggaagc
     9901 ggagaaggcg agggcgaggc tggcaaagct cagtcagact tgataggtaa gcgtttcggc
     9961 agccacggca caaacgcatt cgtcgtttgc tgataatggc ggtactccgc cccacgggtc
    10021 ttcagagcct gctgctccgc caagggaaca ccagttacac gcaggagcgc ccatcccata
    10081 ccagcgggac caatgagcgt caggggccaa ccgggcgctc caatcgccat cacgacatag
    10141 ctccaccagt gaagccattc gaagaaataa ttgggatggc gcgagtaata ccaaagacca
    10201 tcccggcaga cccgatcacg attccacggt tgtgcacgga accgcgccaa ttgccggtcg
    10261 gctgcggcct cgcccgaaat ggccgcgatc cacatcaaga aacctgcgag ctcgacgaat
    10321 ccgaacgggg gcctgggatt ttgaatgagc accagaaatg ggagcgaaaa ggcggccacc
    10381 gctgcggctt gcaactggaa gtacccgaac atcttggctt gctccgaagc cccccagcta
    10441 cgacgcatct cttggtaccg ggcgtcttcg gctttaccga tcacacgatt gaaaaggagg
    10501 aagaggccga gccggccggc atagacgcag accatgaagg cgaccagcag cttccgttcc
    10561 ggttccccga atccataccg ggtgtaccag agcacgatca cgatcaatcc ggcacaaaag
    10621 ccgacatcgg cgatcgaggc attgcgaagg accacctgac ccccccagag gacaagcatg
    10681 aggacgacca tggccccata gccaaccatg acgaggaaaa gggggtcgga attcatggca
    10741 acgggtaatc ccaaagatca tgtccccggc aacttcgttc gcccagccag gattgcattc
    10801 ccggaatcgg aaaagacaga acatgctgga tctttgtggt ggacaatgag agatcgggcg
    10861 gacgtggagc acccgtatga tcacgcgaag agcctttgac caaccggccc tgcagctccg
    10921 ggaaccactc gaggagaagc tggccgattt cccagcgcga cagacgctcg cggccaccga
    10981 gatgatagag gcctgttctc ccgctacaga tcagttccca tatcgcacgg gcaacaactc
    11041 cggcgggcag cggacagcga aattcatccg catacagcgt aatggaactg ccagccttgg
    11101 ccgcccggca catgtcttcc acaaaacttc ggtcacgccg caatgaggtt cccgcggtga
    11161 gcacgatccg aacgacgctg tgacggacat tctgcaaaac gatacgctcg gcggcaagct
    11221 tcgtcgcacc atacacattg atcggagctg gtgcatcgg
//
LOCUS       DASTQY010000229         8365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1121108, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000229 DASTQY010000000
VERSION     DASTQY010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121108"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /gene="pyrG"
                     /locus_tag="VFR82_07520"
     CDS             complement(<1..325)
                     /gene="pyrG"
                     /locus_tag="VFR82_07520"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448957.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HEU4501483.1"
                     /translation="MGKYIFVTGGVVSSLGKGLASASIGNLLESRGLKITFLKLDPYI
                     NVDPGTMNPYQHGEVYVTDDGAETDLDLGHYERYTSLTLTKENNYTTGRIYNSVITKE
                     RRGDYL"
     gene            complement(372..1157)
                     /gene="kdsB"
                     /locus_tag="VFR82_07525"
     CDS             complement(372..1157)
                     /gene="kdsB"
                     /locus_tag="VFR82_07525"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEU4501484.1"
                     /translation="MSKQKHYVTVVIPARYGSSRFPGKPIAMLGQKPIIQHVYEQAAA
                     CRAVSEVVVATDDRRIQETVVQFGGQAVLTMGDFRSGTDRVAAVARLRTGQFILNLQG
                     DEIPVDPDLLTDLIEPFVESGAEMGTLKRALHTTEDLQNPAVVKVVTDRHGRALYFSR
                     APIPLVRDDPSRQAVKGLHYIHLGVYIYTRETLLRLTDLPTGMLEDSEKLEQLRALDH
                     GITIHVWETGHESLRIDTPADATSAGESLRRMALNKLETLNQL"
     gene            complement(1150..2214)
                     /gene="rfaE1"
                     /locus_tag="VFR82_07530"
     CDS             complement(1150..2214)
                     /gene="rfaE1"
                     /locus_tag="VFR82_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEU4501485.1"
                     /translation="MGAVREGDSVNKPARDSTGAAGSSVRSDKPSANSLRRHIQRFPH
                     ASVLVIGDLILDHYIWGRVSRISPEAPVPVVHVESESMRLGGAANVFNNILALGGQAD
                     LCGIIGSDEAGRLLLKELGTKRSGHRGIVVDGNRPTIKKSRVIAHNQQVVRYDVERKG
                     EPKPQLQKKMLRYVESCLREVTCLVVSDYAKGVVTAGLMADLVRLAALRKVPVIVDPK
                     VEHFGFYKGVTVITPNHLEATQAAGLHGDGDQTIQEAGETIRQRLGCQAVLVTRGERG
                     MSLFEGNKDAWHIPTQARQVYDVTGAGDTVVGTLALALSIGASMREAAMLANHAAGIV
                     VGMVGTATVSTKQLSEALGE"
     gene            complement(2184..2939)
                     /gene="lepB"
                     /locus_tag="VFR82_07535"
     CDS             complement(2184..2939)
                     /gene="lepB"
                     /locus_tag="VFR82_07535"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU4501486.1"
                     /translation="MSVDPNLPSSDELASRASDATTGVEVSTSVQPVRKSVFREYAEA
                     IVVAMLLAFAIRVFVVQAFKIPSGSMIPTLLIGDHILVSKLAYGIQWPSDCKFQVAFP
                     PVNCYASHALIEFGKPQRGDIIVFRFPEDEEKDFIKRIVGTPGDTVQLKNKVVVVNGT
                     PLDDKAFTQRIDSGVIDGTINPRDNFGPVTVPPDSYFVMGDNRDQSLDSRFWGFVREE
                     KIRGKAFRIYWSWSGQGQWQEWVRWGRFGKAIQ"
     gene            complement(2936..4735)
                     /gene="lepA"
                     /locus_tag="VFR82_07540"
     CDS             complement(2936..4735)
                     /gene="lepA"
                     /locus_tag="VFR82_07540"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEU4501487.1"
                     /translation="MQSLIRNFSIIAHIDHGKSTLADRFLEATGAVTAREAKDQILDA
                     MDLERERGITIKAHAVAIRYKAKDGNTYALHLIDTPGHVDFTYEVSRSLAACEGSLLL
                     VDATQGVQAQTIANVNLAMANNHVIIPVINKIDLASADVENTKHSISEVLMLEASHAI
                     PISAKEGRGVPEVLEAIIERIPPPSGDPQAPLKALIFDSWFDNYQGVIVLTRVVDGAM
                     RPGMKIKVMSNGRTFEVMEVGHFTPKRIKKAELLTGEVGYLCANMKEVADVKIGDTIT
                     DAVQPTAEPFPGYKEVKPLVFCGLYSTDTAKYEDLRDALIKLRLNDSSFVYEPETSLA
                     LGFGFRCGFLGLLHMEIIQERLEREYGLTLITTAPTVVYRVTTTKGEVLEIDNPAELP
                     PPNSIERFEEPFILATVITPERYMGAILQLCQERRGTQRSIHFLDPTRVLISYEMPLN
                     EVILDFYDKLKSRTQGYASLDYELLGYRESDLVKLDILLNGEAVDALSFITHKERSYP
                     RGRQLAEKMKELIPRQMFEIAIQAAIGNKVIARETIGAMKKNVTAKCYGGDITRKRKL
                     LEKQKEGKKRMKAVGSVEVPQEAFLAILKVGEE"
     gene            4890..6260
                     /gene="bioA"
                     /locus_tag="VFR82_07545"
     CDS             4890..6260
                     /gene="bioA"
                     /locus_tag="VFR82_07545"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEU4501488.1"
                     /translation="MTRKKQAPSLGVWDRQYLWHPFTQMQEWEQEEPVIIERGKGSYL
                     FDTEGNSYLDGTSSIWVNLHGHRHPVLDRALDDQRSKVAHTTLLGLSNPPAILLAREL
                     VRIAPRGLARVFFSDNGSTAVEVALKMAVQYWKQARPEAGPKHSFVHLRLAYHGDTIG
                     AVSVGNIDLFHARFKPLLFPTHTVEPPYCYRCPLKLTFPECRLACLDPIEQVLRDHRG
                     ELAGFIIEPLMQAAAGMIAQPPGYLKRIRELCTKYEVLLIADEVATGFGRTGAMFACH
                     QEGVTPDLMAISKGLTGGYLPLAATLTTEDIYRAFLGKYEEFKTFFHGHSYTGNPLGC
                     AVALANLQVFRKEKTLGKLRSKITLLTRLLKPLHQLPHIGQVRQQGFMVGIELVQDRS
                     TKEPYPLESRIGHRVAQEARRRGLLLRPLGHIIVLMPPLSVSVDELTRMVSIVSRSIA
                     AVTQHE"
     gene            6401..7639
                     /locus_tag="VFR82_07550"
     CDS             6401..7639
                     /locus_tag="VFR82_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501489.1"
                     /translation="MKRSLTILLVCLTLNLLTSLSWGSEEQPGERSGQRWSTLLTEPV
                     TSPALLSSGHRSGQVFETTVTSTFMVIPPTSTRLRDVSPETEGPRVKGLVAKMSWLKG
                     RFVTESEVANSYGGNDWWEHKIPGDNRVDASRQMVRLGVTGTTGALSYGLNYRKAGQA
                     FLNAPDQSSREMWGEWRANWVTMRTVVGQLWNNVAEDISRPRQVQTYGRMSMGFRRAS
                     WPELSLTYGRNSFSSALEPAGILPQRTQSHTLEGAVAYQSLRWNVRLSSTYALASDLL
                     HGGTESNVRMQLLSASFRPLNTFTISPMLAYREEIQDASGMRIDGPSASLALHYKQSR
                     RLLVSAIGNYANCRSNDRQIDNENVGGKGIVAWDVQQSTRWSTLVALEAGYTRVSNRV
                     TPAADTEDISGLLRIVLAAL"
     gene            complement(7655..>8365)
                     /locus_tag="VFR82_07555"
     CDS             complement(7655..>8365)
                     /locus_tag="VFR82_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEU4501490.1"
                     /translation="INPQATIVDLSHRVTSHDVQDGAYLWKCCYRYFPEGTIHVAIVD
                     PGVGSERRPLVARSSRYYFFAPDNGLLTHVFDQEREVEVREVENKQYRLDSEGHTFDG
                     RDVFAPGAAWLTKNLPVSSFGRLVTDARRFGVTQPHWEHMALVGEVAYVDAFGNLITN
                     VSSGHVKEVREITKRPNPLIRIVGHSIDGLVRCYAEGDRTMPHALINSDGQLEIFCKE
                     TSASSLLKVGRNEPVKLS"
BASE COUNT         1785 a         2470 c         2275 g         1835 t
ORIGIN      
        1 caagatagtc gccacgccgt tccttcgtga tgacggaatt gtagatacgg ccggtcgtgt
       61 agttattctc tttcgtcaac gtgagcgagg tgtaccgttc gtaatgacca agatccaaat
      121 ctgtctctgc gccgtcgtcg gtgacataca cctcgccgtg ctgatatgga ttcatcgtgc
      181 cgggatcgac gttgatgtag ggatcaagtt taagaaacgt gatcttgagg ccgcggcttt
      241 ccagtaaatt gccgatcgag gccgaggcta gccccttgcc aagggatgac acgaccccgc
      301 ccgtgacgaa aatgtatttg cccattatgg tctccatccg tggcgatcga gcgaacctaa
      361 tccgcattca gtcataactg attcaacgtt tcaagcttgt tgagcgccat tcgtcgcaag
      421 ctttcaccgg cactcgtcgc gtcggccggc gtatcgatgc gcagggactc atgtccggtt
      481 tcccagacat ggatcgtgat gccatgatcc agagcacgca attgctccaa tttttcagag
      541 tcttccagca tcccggtcgg caagtcagtc agccggagca gcgtctcgcg ggtatagatg
      601 tagaccccca agtggatata gtgcaacccc ttcacggcct gccgggaggg atcgtcgcga
      661 accaagggaa tcggagcacg tgaaaaatac agtgcgcggc catgccgatc ggttacgacc
      721 ttgaccacgg cggggttctg caggtcctca gtggtgtgga gcgcgcgctt caacgtgccc
      781 atctcggcgc cagactcgac gaacggctcg atcaaatccg tcaggaggtc gggatcgacc
      841 ggaatttcat cgccttgcag attgaggata aactgaccag tgcgtagccg tgccacggca
      901 gcgacacgat ccgtcccgct ccgaaaatcg cccatggtta acacggcctg tcccccgaat
      961 tgcacgactg tctcttgaat ccgacgatcg tcggtggcaa cgacgacctc agagactgca
     1021 cggcacgccg ccgcttgctc ataaacatgc tggatgatcg gtttttgccc aagcatggca
     1081 atcggtttcc ccggaaaccg ggacgagccg taccgggctg gaatcaccac agtgacgtaa
     1141 tgtttctgct tactcaccaa gtgcctccga taactgcttc gtcgacacgg tcgcggttcc
     1201 aaccatgccg accacgatcc ccgcagcatg attggccaac attgcggcct ctcgcatcga
     1261 ggcgccgatt gacaatgcca acgccagggt cccaacgacc gtatcgccgg caccggtcac
     1321 atcatatacc tggcgcgctt gcgtggggat atgccatgca tccttgttgc cttcgaacag
     1381 actcatgccc ctttcacctc gcgtgacgag caccgcctgg caacccaagc gctggcgaat
     1441 cgtttcgccg gcctcctgaa tcgtttgatc gccatcaccg tggagtccgg cggcctgggt
     1501 cgcttccaga tgattgggcg tgatcaccgt cacccctttg taaaagccga aatgctcgac
     1561 cttcggatcg acaatgacag ggaccttccg cagcgccgcg agccgaacga ggtctgccat
     1621 gagccccgcc gtcaccaccc ctttcgcata gtcggacacc accaaacagg tgacttcccg
     1681 gagacacgat tccacatacc gcaacatctt tttttgcaat tgcggcttgg gctcgccctt
     1741 gcgctcgacg tcatagcgaa caacttgctg attatgggcg atcacccggg actttttgat
     1801 cgtaggccgg ttcccatcga cgacgatgcc tcgatgcccg gaacgcttcg tccccaattc
     1861 cttcaacaac aggcgtccag cttcatccga gccgatgatg ccgcagaggt ccgcttggcc
     1921 gcccaaggcc agaatattgt taaagacatt ggcggctccc ccgagccgca tggattccga
     1981 ttcgacatga accacgggca ccggcgcctc gggggaaatc cggctcacgc ggccccaaat
     2041 gtaatgatca agaataagat cgccgatgac gaggactgaa gcgtgtggaa atcgttggat
     2101 gtgtcgacga agactgttgg ccgagggctt gtcagagcgg actgaagatc ccgctgcccc
     2161 ggtcgagtcc cgcgccggct tgtttactga atcgccttcc cgaaccgccc ccatcttacc
     2221 cactcctgcc attgcccttg gccgctccag gaccagtaaa tgcggaaggc cttccccctg
     2281 atcttttcct cgcggacaaa tccccaaaac cggctgtcca gactttgatc acgattgtct
     2341 cccatgacga aataggaatc gggcggcacc gtcaccgggc cgaagttatc gcgtgggttg
     2401 atcgtgccgt caatcactcc tgaatcgatg cgctgtgtaa acgccttgtc gtccagcggc
     2461 gtcccattga ccacaaccac tttgttcttg agctggaccg tatctccagg agtgccgaca
     2521 atccgtttga tgaaatcctt ttcttcatct tccgggaacc ggaagacgat gatgtcaccc
     2581 cgctgcggtt ttccgaactc aatgagtgca tgagaggcgt aacaattcac cggagggaag
     2641 gcgacttgga atttgcagtc gctcggccat tggatgccat aggccagttt actgaccagg
     2701 atgtgatcgc cgatcaagag cgtgggaatc atcgaaccgg aaggaatctt gaatgcctgg
     2761 acgacaaaga ctcggatcgc aaaggcgagc agcatcgcga cgacgatggc ttccgcgtat
     2821 tctcggaaaa cggacttccg gaccggctgc acgctggttg acacctctac tcctgtcgtg
     2881 gcgtcggatg cacgggacgc gagctcgtcg gagctgggaa gattcggatc gacgctcatt
     2941 cttcaccgac tttcaagatc gcgagaaagg cttcctgcgg aacttcaacg ctgccgacgg
     3001 ctttcatccg tttctttcct tctttttgtt tttcgagcag tttgcgcttg cgcgtaatgt
     3061 caccgccgta acactttgcc gtgacgtttt tcttcatcgc gccgatcgtc tcgcgtgcga
     3121 tgaccttgtt gccgatcgcc gcttgaatcg caatctcaaa catttgcctg ggaatcagct
     3181 ccttcatctt ttccgccagc tgacgccccc gcggataaga gcgctctttg tgcgtaataa
     3241 aagacagcgc gtctaccgcc tctccgttta ataaaatgtc gagcttgacc agatccgatt
     3301 cacgatagcc gagcaactca tagtccagcg acgcataccc ttgcgtgcgg gacttcagct
     3361 tgtcgtagaa atctaagatc acttcgttca agggcatttc gtagctgatc aggacgcgag
     3421 tgggatcgag aaaatggatg ctgcgttgcg tcccacgccg ctcctgacac agctgaagaa
     3481 tggctcccat gtaacgctcc ggcgtgatca ccgtcgccag gatgaacggt tcttcaaacc
     3541 gctcgatact gttggggggc ggcagctcgg ccggattgtc tatttccagg acctcaccct
     3601 tagtggtcgt gacacggtac acgaccgtgg gagccgtcgt gatcaaggtc agcccatact
     3661 cccgctcgag tcgctcctgg atgatttcca tgtgcagtag accgagaaag ccgcagcgga
     3721 acccgaaccc gagggccagc gaggtttccg gctcatagac aaacgacgaa tcgttgagcc
     3781 gcagcttgat gagcgcatcg cgcaaatctt cgtatttggc cgtgtcagtg gaatacaggc
     3841 cgcagaagac cagtggcttc acttccttgt agccggggaa cggctcggca gtcggctgga
     3901 cagcgtcggt gatggtgtcg ccgattttga catcggccac ctccttcatg ttggcgcaga
     3961 gatagccgac ttctcccgtc agcagttccg ctttcttgat tcgcttcggc gtgaagtggc
     4021 ccacttccat gacctcgaag gtccggccgt tcgacatgac tttgattttc atcccaggac
     4081 gcatcgcccc gtccaccacg cgggtcaaca cgatcacgcc ctggtaattg tcgaaccagg
     4141 aatcgaagat cagggccttc aacggtgcct gcggatcacc tgagggggga ggaattcgct
     4201 cgatgatggc ttcgagtacc tcggggactc cacggccctc cttcgcactg atcgggatgg
     4261 catggcttgc ttccagcatc agcacttccg agatggaatg tttggtgttc tccacgtccg
     4321 cactggccag atcaatttta ttgatgaccg ggatgatgac atggttgttg gccatcgcca
     4381 gattcacatt ggcgatcgtc tgagcctgga cgccttgggt cgcatcgacc agaagcaacg
     4441 acccctcgca ggccgccagg cttctggaca cttcataggt gaaatcgacg tggccgggcg
     4501 tgtcgatcag gtgcaaggcg taggtgttcc cgtctttggc cttgtaacgg atcgccacgg
     4561 cgtgcgcctt gatggtaatt ccccgctcac gttccagatc catggcatcg aggatctggt
     4621 ccttggcctc tcgggccgtg acagcgcctg tggcttcgag gaaccggtca gcgagggttg
     4681 atttaccgtg atcgatatgg gcaataatcg agaaattgcg tatgaggctt tgcaaatctg
     4741 tcgctcattt cttcaataaa atcggttcat tatagtaact gcctcccagg ttgtcaaagt
     4801 aaagagcccc tcgtataatc cgcgccgcgc ccgcactagc actaggtttt ccggcatcac
     4861 cttcttacgg tttccgccga gcaactggca tgacccgcaa aaaacaggcg ccctcgctgg
     4921 gagtgtggga ccggcaatat ctgtggcacc cttttaccca aatgcaggag tgggaacagg
     4981 aggagccggt cattatcgaa cgcggcaaag gatcgtatct cttcgacacc gagggtaaca
     5041 gttatttgga tggcacctcc tccatttggg tcaaccttca tggtcatcga catccggttc
     5101 tcgaccgtgc cctcgatgac cagcgaagca aggtggcgca cacaactctc ctcggtctct
     5161 cgaatcctcc cgccattcta ttagcacgcg aactggtgcg gatagccccg cgaggactgg
     5221 cacgggtatt tttctcggac aatggttcga ccgcggttga ggtggccctc aaaatggccg
     5281 tgcaatactg gaagcaggcg cgcccggagg caggacccaa acacagtttc gtgcacttaa
     5341 gactggccta tcacggcgac acgatcggag ccgtcagcgt cggaaacatc gacctatttc
     5401 atgcgcgatt caaaccctta ctcttcccca cccatacggt cgaacctcct tactgctacc
     5461 gctgcccgct caagctgacg tttccggagt gccgccttgc ctgcctcgac cctatcgaac
     5521 aagtgttgcg tgatcatcga ggtgaattgg ctggcttcat cattgaaccg ctcatgcagg
     5581 cggcagcagg catgattgca cagccaccgg ggtacttgaa acgcatcaga gagctctgca
     5641 cgaaatacga ggtcttgttg attgccgacg aagtcgcgac aggattcggc cggaccgggg
     5701 ccatgtttgc gtgtcaccag gaaggagtta cccctgatct gatggcgatc agcaaagggc
     5761 taacgggagg ctacttgccg cttgccgcga cgttaacgac ggaggatatc tatcgtgcct
     5821 ttttagggaa gtatgaagag ttcaaaacat ttttccatgg ccatagttac accggcaatc
     5881 cgctcgggtg cgcggtggcg ttagccaacc tgcaagtgtt ccggaaagag aaaactttgg
     5941 ggaaattgcg ctccaaaatc accctgctga cccgactgct caagcctctc catcaactcc
     6001 ctcatatcgg gcaggttcga cagcagggct tcatggtcgg aattgaactg gtacaagacc
     6061 gttcgacaaa agaaccctat ccactcgaaa gcagaatcgg acaccgagtg gcgcaggagg
     6121 ccagaaggcg aggtcttctt ctcagaccgc tcggccacat tattgtgttg atgccacccc
     6181 tgtccgtcag cgttgatgag ctgacccgca tggtcagcat agtatcccga tcaatcgccg
     6241 cggtgaccca gcacgagtag cccctcgttc ctcccccgca tcgtttcgcc cgctccgaca
     6301 gacgcaattc cacaccaaaa tcacatctgt tttccggcat ttagacccta atcttcgact
     6361 gctaaatttt atagtgttta cggctcttca aggggtcggc atgaagcgtt cgctgacaat
     6421 cctgttggtg tgtctgacgc tcaacctcct cacttccctc agctggggca gcgaagagca
     6481 accaggcgaa cgaagcgggc agcgctggag tactcttctg acggaacccg tgacgtcacc
     6541 cgccctgctc tcaagtggtc accgctccgg gcaggtcttt gaaacgacgg tcacctcgac
     6601 ttttatggta attccaccga cgtccacacg cttgcgcgat gtctcacctg agacagaggg
     6661 tccccgagtc aagggacttg tggccaaaat gagctggctc aagggaaggt ttgtgactga
     6721 atcggaggtc gcgaacagtt atggaggaaa cgactggtgg gagcacaaga tcccgggcga
     6781 caaccgggtc gatgcctcga gacaaatggt tcggcttggc gtcaccggga ctacaggcgc
     6841 tctcagctac ggcttgaact acagaaaggc cggtcaagcc ttcttgaatg ccccggacca
     6901 aagcagtcgg gagatgtggg gagaatggag agcgaactgg gtcacgatgc gcaccgtggt
     6961 gggacagttg tggaacaatg tcgcagaaga tatttcacgc ccacgacagg tccaaaccta
     7021 tgggcgaatg agcatgggtt ttagacgggc gtcgtggcct gagttgagcc tcacttatgg
     7081 acggaactcc ttctctagtg cgctcgaacc ggcaggcatt ctcccgcagc gtactcagag
     7141 tcatacgctc gaaggcgcgg tggcctatca gagtctgcgg tggaacgtga gactcagctc
     7201 cacctatgct ctcgccagcg acctgctcca cggcggaact gaaagcaacg tccgcatgca
     7261 actcctcagc gcctcgtttc gaccactcaa tacgtttacg atctcgccca tgctcgcgta
     7321 cagggaggag atccaggacg cttccggcat gcgcatcgat ggcccttctg cttcgctcgc
     7381 gctccattat aaacaaagtc gtcggctctt ggtcagcgcc atcggcaact acgccaattg
     7441 ccgttcgaac gaccgacaga tcgacaatga aaatgtggga ggtaagggca ttgtagcctg
     7501 ggacgtgcag caatcgacaa gatggagcac cttggttgcc ctcgaagcgg ggtacactcg
     7561 cgtgagcaac cgggttacgc ccgccgctga cactgaggat atttcaggac tgctacgtat
     7621 cgtcctcgcc gctctctaga gcactgcggc gacactacga cagcttgacg ggttcgtttc
     7681 gtcccacctt gagcagcgaa ctcgcgctcg tttccttgca gaagatttct aactggccat
     7741 cactattgat caaggcatgg ggcatcgtgc ggtccccttc ggcgtagcat cgcacaagtc
     7801 catcaatgga gtgccctacg atcctgatca gcgggttcgg ccgtttggtg atttcccgca
     7861 cctccttcac gtggcctgac gagacattcg tgatcagatt gccgaatgca tccacgtagg
     7921 caacctctcc aacgagcgcc atgtgttccc aatgcggctg agttaccccg aacctgcgcg
     7981 cgtccgtcac cagccgccca aacgaagaca ccggtagatt cttggtgagc catgcggctc
     8041 ccggggcaaa gacatcacgc ccgtcgaatg tgtgaccctc ggaatcaagg cgatattgct
     8101 tgttttccac ttctcggact tcgacttccc gttcctggtc gaacacatgc gtgagcaatc
     8161 cgttgtccgg cgcaaaaaag taataccgtg aactccttgc cacaagggga cgtcgctcgc
     8221 ttcccacacc aggatcaacg attgcgacat ggatggtgcc ttcggggaaa tatcgatagc
     8281 agcacttcca gaggtaagcg ccatcttgga cgtcgtgaga ggtcacccga tggctcaggt
     8341 ccacgatggt cgcctgaggg ttgat
//
LOCUS       DASTQY010000230         4006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1335773, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000230 DASTQY010000000
VERSION     DASTQY010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335773"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1043..>4006
                     /locus_tag="VFR82_07560"
     CDS             1043..>4006
                     /locus_tag="VFR82_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ndvB"
                     /protein_id="HEU4501491.1"
                     /translation="MTNYPELEEPIRAELFGVERLEQHAESLAAAQIITGDLRRGRLL
                     TPRVLENGRILVKSYRTIARAIREEKTITPAAEWLVDNFHIVDEQLREIIDDLPPGYY
                     RQLPKLASGHLENYPRVFGVAWAFVAHTDSRFDPEMLRRFVAAYQRVQPLTIGELWAV
                     AISLRVVLVENLRRLAERIVHSRAARLEADELADGLLGTGLSDVDTSATALRRFEHRP
                     LATAFAVQLVQRLRDLDPKVRPVLLWLDDRLAAQGTTANDIVHAEHQQQAAMSVTVRN
                     IITSMRFTSAFDWADFFERVSLVDDILHREPRYTEMDFVTRDTYRHAIEDLSRGSGFS
                     EIDVTTRVVDRARRAVMEPHASEQVERHSDPGYYLISQGRRAFERELDYRVSWKRRFL
                     RWYVRASVPGYLGSLAVVTAIIMALTLWRSVEAVETTAGLVLLGLLALVPASDLAMAL
                     INRTVMAILGPRPLPRMALRNGIPKELRTIVAIPTLLTNQQGIEEQVERLEVHYLANS
                     DDDLRFALLSDWRDAPSESIAGDAELLATAVEGIARLNKRYGPASGGGSRFVLFHRRR
                     VWNKSEHTWMGWERKRGKLHELNQLLRGSTHTTFMSVGGQAPEGIPSVRYVITLDADT
                     RLPRGAARRLIGTMAHPLNRPRLDPLAGRVVEGYGVVQPRITPSLPTDGEGSCFQRTF
                     SGPSGIDPYASAVSDVYQDLFREGSYTGKGIYEIDAFEAALADKVPDNAMLSHDLFEG
                     LFARTALATDVELFEAFPSHYEAAAARQHRWARGDWQLLPWLLGRGHTGSEPHRAVMI
                     PAIGRWKILDNLRRSLSAPAAFLALLAGWLLPQGAAWVWSSFILATIAIPSLLSFVLG
                     LYPRRRGISIRSHVRGAVTDLKLAATQIALTVTFLAYQAWLMSDAILRTLSRLVITRT
                     HLLEWVTAAQAEDAYTCNLWGMYRRMAGGIVLTVMAACGVALFGWNDSWAAAAPFILL
                     WMAAPAVARWVSLPPR"
BASE COUNT          768 a         1182 c         1166 g          890 t
ORIGIN      
        1 tgcgtcagag atgagggctc caggctgcgc ctaaatcatt cttcaattgg tagtcaggca
       61 gccgcctttg aatgaagacg tgcagtccaa tcgtattctt atcttcttcc agtgcctgag
      121 caattttgac cacccccagc agtttccctc gtactattat tccgctcggt ctacccgttc
      181 ctcctcaatt cactcttgta aacagatggt ttcagtgccg taggctcttc tatgctattg
      241 gaagacaatt tacctgggac cggccaacaa gcgcgaacac gcgaaaccaa gatccgcgcg
      301 ctgcacacgc ccgcatgccg gtgcgcatgg caacgcctgg ctcgacagcg atggttggat
      361 catctgctca gctgggtggg atggtaccca tggcagtgca ggcggtgcca taagcgctcc
      421 tacgtccgtt ttcgattctg agctctccct tgcttccgcc aggcacggac cacatccaaa
      481 gcgcacgtga gcagttttga ccacctactc ggacctctat tcagcgagaa tgtgtgtctg
      541 ccttcatcac aggctctaca cacgatttcg cgcgttaatg gaggggcgtg aacaccgctg
      601 gctgtctctg ctatcaggtt gcaccaatcg gctatggggg gagtgaccta tggaactgtc
      661 ccagctgtaa gcaaacctac caccgacaat acgaggaaga ttctggcatg tggatctggg
      721 cggtatcttc tacacgacga actgcctctc tacataccaa ctgccgacgg cctcttgtaa
      781 ggccgtaccg ggcgacatac tgaccaagac ggttgccaat ctatctactg atcgacgaag
      841 caaaatgtct acgccacatc ttgcttggaa caaaaaagat cgcggttcga gcgcggaagc
      901 atgatccgta tacggaatta tagtgaatcc ggggatcgtc ttcggattcg atcacgacga
      961 gttctattgg cctcgcgctt tcaataaatt agcgttcaag ccctctatct ggcagcgact
     1021 tcctaatcag gacggttcat ctatgaccaa ttatccagag ctggaagagc cgattcgggc
     1081 agagcttttc ggtgtcgagc ggctcgaaca gcatgctgaa agtctcgcgg ctgcccagat
     1141 cattaccggc gacttacggc ggggccggtt actcacgcca cgagtcttgg agaatggacg
     1201 gatcttggtg aagtcctacc ggaccatcgc ccgcgcgatt cgggaagaga aaacgatcac
     1261 ccctgccgcc gaatggttgg tcgacaactt tcacatcgtg gacgagcagc ttcgcgagat
     1321 catcgatgat ttacctcccg ggtattacag gcaattgccc aaactggcat caggccacct
     1381 ggagaattat ccacgggtct tcggagtagc ctgggcattc gtcgcccaca cggacagtcg
     1441 attcgacccc gaaatgctgc gccgatttgt ggcggcctac cagcgcgtgc aacctctgac
     1501 gatcggggaa ttatgggcgg tggccatttc gctgcgtgtc gtactggtgg aaaaccttcg
     1561 cagactggca gagcggatcg ttcacagccg cgcggcccgg ctggaagccg acgaactcgc
     1621 cgacggtttg ctgggtaccg ggttatcgga cgtagacact tcggcaacgg ctctccgtcg
     1681 ctttgagcac agaccgttgg caacggcgtt tgccgtgcag cttgtccagc gactccgtga
     1741 tctggatccg aaggtgcgcc ccgtcctcct gtggctcgat gaccgccttg ccgcgcaggg
     1801 tacgaccgcc aacgacatcg tgcacgccga gcaccagcag caggcggcta tgagcgtgac
     1861 tgtgcggaac atcatcacca gcatgcgatt cacgtcggca tttgattggg cggacttctt
     1921 cgagcgcgtc agcctggtgg atgacattct tcatcgcgag cctcgataca ccgaaatgga
     1981 ttttgtcacc cgggatacct accgccacgc catcgaggat ttgtcccgtg gctcgggttt
     2041 ctcagaaatc gacgtgacga cccgggttgt ggatcgcgcc aggcgggcgg tgatggagcc
     2101 gcatgcgagt gaacaggtgg agcggcattc agaccccggc tattacctca tctcgcaagg
     2161 acgccgggcg tttgagcgtg aactcgacta cagagtgtcc tggaagcgcc gattcctgcg
     2221 ttggtatgtc cgggcttccg tgccgggcta cctcggatcc ctcgcagtgg tgaccgccat
     2281 aatcatggcc ttgacgctgt ggcgatctgt ggaagccgtc gagacaacag cgggccttgt
     2341 gctccttggt ctccttgcgc ttgtgccggc ctcggatctc gccatggcgc tgatcaatcg
     2401 cacggtcatg gcgatcctcg gaccgcggcc attgcctcga atggcattgc gcaacggtat
     2461 tcctaaagag ttgcgaacga tcgtggccat tcccacgttg ctcacgaacc agcagggtat
     2521 cgaggagcag gtggagcgat tggaagtcca ttatctggcg aattcggatg atgacctacg
     2581 cttcgccctg ctctcagact ggagagatgc cccaagcgaa agtattgcag gcgatgcgga
     2641 gctactcgcg acggcagtcg aagggatcgc gcgcctgaat aaacggtacg gccctgcgtc
     2701 cggcggtggt tcgcgttttg tgctgtttca ccgcaggcgc gtctggaaca agtccgagca
     2761 cacctggatg gggtgggaac ggaagcgagg caagctgcat gagttgaatc agttgttgcg
     2821 tggctcgaca cacacgacgt tcatgtccgt cggcgggcag gcccccgaag gcattccttc
     2881 tgtgcggtac gtcatcacgc ttgatgcgga cacacggttg ccgcgcggag ccgcacgccg
     2941 cttgatcggt accatggccc atcctctgaa ccggcctcgg ctcgaccctc tcgctggccg
     3001 cgtcgttgaa gggtacggag tcgtgcagcc acgaattacg ccctcgctac cgaccgatgg
     3061 tgagggctcc tgttttcaac ggacgttttc gggccccagc gggatcgatc cctatgcatc
     3121 cgccgtgtcg gatgtgtacc aggatctgtt ccgtgaaggg tcttataccg gcaaaggcat
     3181 ttacgagatc gacgctttcg aggccgcctt ggctgacaaa gtgccggaca atgcaatgct
     3241 cagccacgat ctctttgaag ggctcttcgc gcgcacggcg ctcgcgaccg acgtcgaact
     3301 gttcgaagcc tttccttccc attacgaagc cgcggcagcc aggcagcatc gatgggcacg
     3361 cggcgattgg cagttgttgc cctggcttct cgggcgcgga cataccggat ctgagccgca
     3421 tcgcgcggtg atgattcccg ccatcggtcg ttggaagatt ctcgacaacc tccgccgtag
     3481 cctgtcggct cccgccgcat tcctggcctt gttagccggg tggctgttac cacaaggggc
     3541 tgcctgggtc tggtcctcct ttattctggc cacgatcgcg attccatcgc tcttgtcttt
     3601 tgtcctcgga ctctatccac gccggcgcgg catttccata cgcagtcacg ttcgtggtgc
     3661 agttaccgat ctgaagttag ccgcgactca aatcgcgttg acggtcactt tcctggcata
     3721 ccaggcctgg cttatgtcgg atgcgattct tcggaccctc agtcggctcg tcatcacacg
     3781 gacgcacttg ttggagtggg taaccgcggc acaggctgag gatgcctaca catgcaacct
     3841 gtggggcatg tacaggcgca tggctggcgg tatcgtcctt acagtcatgg ctgcttgcgg
     3901 agtcgcactg tttggatgga acgactcctg ggctgcagcg gccccgttca tcctcctatg
     3961 gatggcggct ccggcggtgg cgcgctgggt cagcctgcct ccgcgg
//
LOCUS       DASTQY010000231         9065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_806923, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000231 DASTQY010000000
VERSION     DASTQY010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9065
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806923"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VFR82_07565"
     CDS             <1..575
                     /locus_tag="VFR82_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4501492.1"
                     /translation="TAGRVLIDGVDIQSFTLASLRAQIGIVSQEVVLFDDTVLNNIAF
                     GKPHATEAEIVQAAKLAYAHDFVERLPQGYRTMVGEKGVKLSGGERQRLAIARAILRD
                     PPLLILDEATSALDTESERVVQLALSNLMEHRTTLVIAHRLSTIQRADRIVVLDRGAI
                     VEIGTHDELLIRDGHYKRLHAMQFQDVPNE"
     gene            568..1200
                     /locus_tag="VFR82_07570"
     CDS             568..1200
                     /locus_tag="VFR82_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU4501493.1"
                     /translation="MSKGLLPSLEAWIKLSVLPPIGAAVIRTLARSMHIETRGQEYVD
                     GLYRRGQRVIIAFWHARQLMMPLTYRGTLAHILISQHQDGEIIARIVERFGFKAVRGS
                     STRGGVEALRELIRLGRAGVDLVVTPDGPRGPAQIAKMGVIQLARASGLPIVPLAFAC
                     SKKNCSPVGIDSWFHTLCRADFFFGGRRFGCRGTVMQQSWNLNGWSCKNC"
     gene            1261..2604
                     /locus_tag="VFR82_07575"
     CDS             1261..2604
                     /locus_tag="VFR82_07575"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEU4501494.1"
                     /translation="MWNLLYNGLLIITAPVILVILLAKPRCRRGLSQRFGFSGPRITP
                     DGRPLVWLHAVSLGEVVAITPLVKELHRRHPTHRFVVSTATETGRDAVEQRLAGIAEH
                     FYAPIDFPWAVSRTIAWLRPVLYVFIETELWPNLLRHLSTLGVPTIMINGRLSSASFA
                     RLRWRPVKSFYRSVLRSMRLCLMQSDRDAERIICLGADPGSVRRTGNIKFDQPLPGEA
                     ERRVTRATLGIEEGAPLLIGGSTHPIEEEQLVEAFVAVLSRHPTTRLLLAPRHIERAE
                     AVEAMVRGRGLAVSRRSAIASEAANGGVRPQVIILDSRGELALIYQEATVAFVGGTLV
                     PIGGHNLLEPASWAKPVLFGPYTDHCVEIADLLTQAGGGRRVTSVGELTKELLRLIEQ
                     HDERVCMGQAAQSVVRQNQGALQATVEAIDGQLSSVETTRSSARSQHSFPVMAGR"
     gene            2613..3695
                     /gene="lpxK"
                     /locus_tag="VFR82_07580"
     CDS             2613..3695
                     /gene="lpxK"
                     /locus_tag="VFR82_07580"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEU4501495.1"
                     /translation="MLTPGQRPTWWLAWAAFPYRILARLRAFGYEAGWLRRHKLLVPV
                     VSIGNLTVGGTGKTPMVIELAERLLRDGKRVAVLSRGYRRTTRAPMLLVSDGVRILAG
                     PIEAGDEPYLIARRCPQAVVAVGVDRYRLGRWVLSQFAVDCFLLDDGFQHMGLHRDID
                     LLLIDATDPSGLDQVLPAGRLREPLEAAGRATALIVTRAEQQTDVEEVLGRLRSAVKS
                     LPMTAQVTFQPQALQRVMTGSGRSLESCEGKSAMLVSGVGHAASFRATAGRLGLKVLE
                     EVVNADHHCYNWHEVARLRQLAADLRVDLVLTTEKDAWKLQPYLTEDDHQWWAVRLMV
                     VWMAGEGALHRTIMHELSRARGGAGD"
     gene            3658..5397
                     /gene="waaF"
                     /locus_tag="VFR82_07585"
     CDS             3658..5397
                     /gene="waaF"
                     /locus_tag="VFR82_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEU4501496.1"
                     /translation="MSSLVLEGEPVIKNPVQRILVRGPNWLGDAVMCEPALRSLKRLF
                     PQAAVTLLVKPSVAGLFERHPSVDRLMLYEDNRRHAGLAGKWRLAGELRRGGFDLAVL
                     FQNAFEAALLTAVAGIPRRYGYATDGRTLLLSEPVAKPDRNKAVHQVQYYWDMLRPLG
                     LTGVPVSPELIVSPEEEKAVAERLKQAGITTEDVLIGVNPGSTYGGAKRWFPERFADT
                     AERLCRRLNESGRRAAVVLLGAKGEEPLGHAIASHLTAPTAVLSGSTTIRDLMAATKR
                     CALLVTNDTGPMHIAAALGVPVVAIFGPTDWRTTSPFGVTHALVRHPVECAPCMLREC
                     PIDHRCMAGVTVQDVYAAANKLLTVPESTAEQGRSSQILRPSPHGILEGYTVFLDRDG
                     TLNEDPGYLKSAADLKLLPGVGQALARLKSAGARLVVVTNQSGIGRGFLTLKDLEAIH
                     ARLEGLLEQNEAALDAIYFCPHHPDDRCRCRKPARGMVDRAASELQLDLRRSYLIGDH
                     VSDMQLAKSVGAKPVLVTTGKVDAPAVERLRAAQAMPDKAVPSMAEAVEWILNDAAAR
                     ASKPAAADAVERS"
     gene            5428..6537
                     /locus_tag="VFR82_07590"
     CDS             5428..6537
                     /locus_tag="VFR82_07590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4501497.1"
                     /translation="MKIFIAESSTAVGGQELAVILHAEGLLKRGHDLRLLLEPQSPIA
                     KMAMDKQLPVTLMTMRKSRYPAAIVSLRALLTHHRPAILQVNSSRDSWIGAMAARLVR
                     PRPKLLRIRHISAPLNRNLMTRILYRHLFDMVVVTGADKTRRELVERDGLAPHRVAAF
                     PIGLDVEYFRPAPPDHDLRLEFGLPKGHLLVGLISYLRSYKGHEYFIEAAEIISGQRD
                     DVTFIIAGEGPEEQSIRTRIERLGLSSKIRLLGFREDLLNVFRSCDVFAIPSVEGDTI
                     PQVLMQALAIGIPVVSTTVGSIPDVVIEGKTGFVVPPRDAGALAERIARLLDDATLRT
                     RMGVEGRSLVQRSYSLEKMLDRMEGVYHRLVKDPA"
     gene            6653..7426
                     /locus_tag="VFR82_07595"
     CDS             6653..7426
                     /locus_tag="VFR82_07595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4501498.1"
                     /translation="MTAGPLSLSIYVIAYNDEPNMRACLESVAGWAAELIVVDSHSTD
                     RTAAIAQEFTDKVYQVDFTGFGALRNQAVAYCSHEWIFSLDTDERMTPELKREIAQVV
                     ELPSAADAYFVPRKNYFLGRWIKHCGWYPDYRQPQLFRKNRLRYREELVHESFDCDGK
                     VGRLSQPALQYPFRDIDHYIAKQDRYSDLMARRMVEQGCRFAAHQLVTHPLAAFAKMY
                     GLRRGFLDGMPGLILSGLYGYYTFMKYAKFWELTKESGR"
     gene            7446..8291
                     /locus_tag="VFR82_07600"
     CDS             7446..8291
                     /locus_tag="VFR82_07600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4501499.1"
                     /translation="MKISGFTFCRNAVKYDYPIVESITSALPLCDEFIVNVGGCGDGT
                     LEVIRSIRDPKIKIVESVWDESLRQGGLIYSQQTNIALAHCTGDWAFYIQADEVIHED
                     DLPVLRRSMEQHAGNPSVKGLLFRYLHFIADYWSTNPWFYHKAVRVIRNNGEIESCGD
                     AVGFHFKPTQQYLQSGPREWLAYSGARMFHYGWVKDPKTMLEKKREQASVYHGANLPP
                     AAAKELTGDAFRFEDYGILKEFRDSHPAVMKTRISSARRWGRRRTRWLNWRFYREVAR
                     RGFRG"
     gene            8307..>9065
                     /locus_tag="VFR82_07605"
     CDS             8307..>9065
                     /locus_tag="VFR82_07605"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4501500.1"
                     /translation="MKRLSWWRRPYFSRRVLEIGPGHNPFAGVTHVLERNVADGRERG
                     GHALIVPAAAHLIVGEATVLPFAAGSFDFVYASHVLEHVEDPEAACREIMRVAAAGYI
                     ETPSPFLEQGLALKDETSPDHWFHRWFVFSLSPGRLVFERKSVEDVRQFCSCADGQFL
                     REFYESLDFRYAQHFFRRSAKTTVFYWKGPFQVEARDHTVDCRQEGRPCRFVGMRRAL
                     IANCNDLLRAGRVRRLPQRFPKSRDVFRKYGQYSL"
BASE COUNT         1740 a         2543 c         2841 g         1941 t
ORIGIN      
        1 caaccgcagg gagagtcttg attgacggag tcgacatcca atcctttacg ctggcctcgt
       61 tgcgggcgca gatcggcatc gtgtctcagg aggtggtgct attcgatgac accgtgctca
      121 acaacatcgc cttcgggaaa ccgcacgcga cggaggccga gattgtgcag gcggcgaagc
      181 tggcctatgc ccacgatttt gtcgaacggc tgccgcaggg gtaccggacg atggtggggg
      241 agaaaggcgt caagctgtct ggcggcgagc ggcagcggtt ggcaattgcc cgtgccatcc
      301 tgcgagatcc acccttactc atcctcgacg aagcgacctc agcgctggat accgaatccg
      361 aacgggttgt gcagctggcg ttaagcaatc tgatggagca tcgcacgaca ctggtgatcg
      421 ctcatcgact gtctaccatc cagcgtgccg accggattgt cgtcttggac cgcggcgcca
      481 tcgtggagat aggaacccat gacgagctct tgattcggga cggccactac aaacggctcc
      541 acgcgatgca attccaagat gtgccgaatg agtaaggggt tgcttccatc gctcgaggcc
      601 tggatcaagc tctcggtctt gccgccgata ggtgcggcgg tcattcggac cctggccagg
      661 tcgatgcaca tcgagacccg gggacaggag tatgtcgacg gactgtatcg aaggggacag
      721 cgcgtcatta tcgcgttctg gcacgcgcgc cagctcatga tgccgctgac ctaccgtggc
      781 acgttggcgc acattctcat cagccaacac caagatgggg agatcatcgc gcgcatcgtg
      841 gaacgattcg gcttcaaggc agtgcggggg tctagcacca ggggaggcgt ggaagcgtta
      901 cgggaactca ttcgactggg acgggccgga gtggatctgg tcgtgacgcc ggatggaccc
      961 agaggtccgg cacaaatcgc gaaaatgggt gtgattcagc tcgcgcgggc ttcggggctg
     1021 ccgatcgtcc cgcttgcctt cgcctgctca aaaaaaaact gttcgccagt tgggatcgat
     1081 tcatggttcc ataccctttg tcgcgcggac tttttctttg ggggccgccg ctttgggtgc
     1141 cgcgggacgg tgatgcaaca gagttggaac ttaaacgggt ggagttgcaa gaactgttga
     1201 atcatctgac tgcgcaggcc gacgaggcag taagccgcag gtcgtgagag cactcgcacc
     1261 atgtggaacc tcttatacaa cggactgttg atcatcaccg caccggtgat cctggtgatc
     1321 ttattggcca agccgcgttg tcgccgggga ttatcacagc ggttcggatt cagcggccct
     1381 cggatcactc cggacgggag accgcttgtc tggctccatg ccgtatcgct cggcgaagtt
     1441 gtcgcgatta ctccgttggt caaggaactc caccgccggc atccaaccca tcgcttcgtg
     1501 gtctccaccg ccacggagac cggacgggac gcggtggaac agcggctcgc gggcatcgca
     1561 gagcatttct atgccccgat cgattttccc tgggcagttt cccgcacgat cgcatggctg
     1621 cgccctgtgc tgtacgtgtt catcgaaacc gagttgtggc cgaacttact gcggcatttg
     1681 tcgacgctgg gcgtgccgac cattatgatc aacgggcgtc tctcttccgc ctcatttgcc
     1741 cgtctccggt ggcggccggt gaaatcgttc taccggagcg tgctccggtc gatgcgactg
     1801 tgcttgatgc agtccgatcg cgatgccgag cggatcattt gccttggcgc cgatcccggg
     1861 tcagttcgcc ggacggggaa cattaagttc gatcagccct tgccgggcga ggccgagcgc
     1921 agagtcacgc gggcgacgct tgggatcgaa gagggcgctc ctctcttaat cggcggcagt
     1981 acgcacccaa ttgaagagga gcagctcgtt gaggccttcg tcgccgtgct ttcgcgtcat
     2041 ccgacgacgc gtctgttgct cgcgcctcgt cacatcgagc gagccgaggc ggtggaggcc
     2101 atggttcgcg gtcgcgggtt ggccgtctca agacgcagcg ccatcgcttc cgaggctgcc
     2161 aacggtggag tgaggccaca ggtgattatc ctcgattcgc gaggagaatt ggctctgatc
     2221 tatcaagagg ccacggtggc ttttgtgggg ggcacgttgg ttcctatcgg gggccataat
     2281 ctattggagc cggcatcatg ggccaagccg gtgctcttcg gcccatacac cgaccattgc
     2341 gtggagatcg ccgatctcct gacgcaggca ggtggcgggc gacgggtgac ctctgtcggc
     2401 gaactgacca aggagcttct gcgtctcatc gaacaacatg atgagcgagt ctgcatgggg
     2461 caggcggccc agtcggtggt ccgtcaaaat caaggggcgc tgcaggcaac cgttgaggcg
     2521 atcgacggtc agctgtcttc ggtggaaact actcgttctt cagcacggtc gcaacactcc
     2581 tttccggtca tggctggacg ctgacgagac ctgtgctcac tcctggacag aggccgacgt
     2641 ggtggttggc ttgggcggcg ttcccgtacc gtattctcgc tcgtcttcgg gccttcgggt
     2701 atgaagccgg gtggttgcgg cggcacaaac tgcttgttcc ggtcgtcagt atcggaaatc
     2761 tcacggttgg cggaaccgga aaaacgccga tggtcatcga gcttgccgag cggctcctgc
     2821 gcgacggaaa acgagtcgcg gtgctgagtc gtggctatcg ccgaaccacc cgggcaccga
     2881 tgctcttggt gtcggacggt gtcagaatat tggctgggcc gatagaggcc ggtgatgagc
     2941 cgtatctgat tgcacgccgc tgtcctcagg cggtcgtcgc ggtcggggtg gatcgatacc
     3001 ggcttgggcg gtgggtactg agccagtttg ccgtcgattg tttccttctg gacgatggat
     3061 ttcaacatat ggggcttcac cgcgatatag atttgctgtt aattgatgcc accgacccct
     3121 ccggcttaga tcaagtctta ccggctggcc gcttgcgcga gcctctcgaa gcggccggtc
     3181 gggcgaccgc gctgatcgtg acgcgagcag agcaacagac cgatgtggaa gaggtgctcg
     3241 gccggttacg gtctgccgtc aagtccttgc ccatgaccgc acaggtcacg tttcagccgc
     3301 aagccttgca gagggtcatg acagggagtg ggcggtccct tgaatcgtgt gagggaaagt
     3361 cggccatgct ggtgagcggt gttggccatg cggcctcatt tcgtgctacc gccggacggc
     3421 ttggcctcaa agttctggag gaagtggtca atgctgatca tcactgctac aactggcatg
     3481 aggtcgctcg tcttcgtcag cttgcagccg atctccgtgt cgatctcgtg ctcacgactg
     3541 agaaagatgc atggaaatta cagccctacc tgactgagga cgatcaccaa tggtgggcgg
     3601 ttaggctgat ggttgtgtgg atggcggggg aaggcgctct gcatcgcacg atcatgcatg
     3661 agctctctcg tgctcgaggg ggagccggtg attaaaaacc cagtgcagcg gattctggta
     3721 cgcggtccga actggctcgg cgacgccgtg atgtgtgagc cggcgctacg cagcctcaaa
     3781 agactctttc ctcaggccgc ggtcacgtta ttggtcaagc cgtcggttgc cgggctgttt
     3841 gagcgccatc cctccgtcga tcgtctcatg ctgtatgagg acaacagacg tcacgcgggg
     3901 ctggcgggca aatggcggct ggccggtgaa ttacggcgcg gtgggttcga tctggcggtg
     3961 ctgtttcaaa acgcgttcga agcggcgttg ctgacagcag tagccgggat tccccggcgg
     4021 tatggctatg cgacggatgg gcgcaccttg ttgttatcgg agccggttgc gaagcctgat
     4081 cggaacaagg cggtccacca agtgcagtat tactgggata tgttaagacc gctgggcctg
     4141 accggtgtgc cggtttctcc cgaattgatc gtctcgccgg aagaagaaaa ggccgtggca
     4201 gaacgcctga agcaggccgg cattaccacg gaagatgtgc tcatcggcgt aaaccctggc
     4261 tcgacatacg gcggagcgaa gcgttggttt ccggaacggt ttgccgatac agctgaacgc
     4321 ttatgccggc ggctcaatga gtccggccgg cgagcagcgg tcgtcttgct cggcgccaaa
     4381 ggggaagagc cgttgggaca tgccatcgct tcacacctga ccgcgccgac ggcggtcctt
     4441 tctggatcca cgacgatccg tgacctcatg gccgcgacca agcgatgtgc cctgctcgtg
     4501 acgaatgaca ccggcccgat gcatatcgca gcggcgcttg gggtcccggt ggtggccatc
     4561 tttggtccga ccgactggcg cacgacgtcg ccgttcggcg tgacgcatgc actcgttcgt
     4621 catccagtcg agtgcgctcc ctgcatgctg cgtgaatgcc cgatcgatca tcggtgtatg
     4681 gccggcgtga cggtacagga cgtctatgca gcggcaaaca aactgctcac tgtaccggag
     4741 tcaaccgccg aacagggacg tagttcacag atcctccgac cttcgccgca cggaattctt
     4801 gaagggtata cagtgttcct tgatcgcgac ggcaccttga acgaagatcc cggctatctc
     4861 aaatcagcgg cggatctgaa actgcttccg ggagtcggcc aggccttggc acgtcttaaa
     4921 tcggcaggag ctcggctggt agtggtcacg aatcagtccg gcattggacg gggattcctg
     4981 acgctcaagg atctcgaggc gattcacgca cgcctggaag gcctgctcga gcaaaacgaa
     5041 gctgcgctcg atgccattta cttttgcccg caccatcccg atgaccgctg ccgatgtcgg
     5101 aagccggctc gagggatggt cgatcgtgcg gcgtccgaat tgcaactgga tcttcgacgg
     5161 tcctacctca tcggcgacca cgtcagcgat atgcaattgg ccaagtcggt tggagccaaa
     5221 ccggtcctgg tcacaaccgg caaagtggac gcgccggccg tcgagaggtt acgggccgcg
     5281 caggccatgc ctgacaaggc cgtgccgtcg atggctgagg cggtggaatg gattctcaac
     5341 gatgccgcgg cccgagcgtc caagcccgcg gccgccgatg ctgttgaacg gtcatgatgt
     5401 tgtgtccctg attctgagca gacctgcgtg aaaatcttca tcgcagaatc cagcacggcg
     5461 gtcggtgggc aggagcttgc ggtcatcctg catgcagagg ggctcttgaa gcgcggccat
     5521 gatctgcgct tgcttctgga accgcagagc ccgatcgcca aaatggcgat ggataagcag
     5581 ctcccggtca cacttatgac gatgcgaaaa tcccgctatc ccgcggccat agtctcgcta
     5641 cgagccttat tgacgcatca ccgtcccgcg attctccagg tcaatagctc acgggacagt
     5701 tggatcggcg cgatggccgc acgattggtc cggcccaggc ctaagctgct tcggatccgg
     5761 cacatctctg cccccttgaa tcgcaatctc atgacccgga ttctctaccg ccatctgttt
     5821 gatatggtgg tggtgacggg agcggacaaa acgaggcgcg agctcgtgga acgagatgga
     5881 ctcgctccac atcgtgtcgc agcttttccc attggtctgg acgtcgaata ctttcgccct
     5941 gctccacctg accatgatct gcgcctcgaa ttcggcctac cgaaagggca cttgctcgtc
     6001 ggcctgattt cttacctccg gtcctataag ggacatgaat atttcatcga ggcggcagaa
     6061 atcatctcgg gccaacggga tgacgtcacc ttcatcattg cgggggaagg ccccgaagag
     6121 cagtcaatca ggacccggat cgagcggctt ggcctctctt ccaaaattcg gctgctgggc
     6181 tttcgcgagg atctcttgaa cgtatttcgc tcgtgcgacg tgtttgctat tccgtctgtg
     6241 gagggagaca ccattccgca agttctcatg caggcgctgg ccatcggaat tccggtcgtc
     6301 tcgacgacgg tcgggtcgat ccctgacgtg gtgatcgagg ggaaaacagg ctttgtcgtt
     6361 cccccgcggg acgcaggcgc gctggcggag cggattgcca ggctgttgga cgacgcgacg
     6421 ctccggacac gaatgggcgt ggaagggcgc tcgttggtcc agcgttctta ttcgctcgaa
     6481 aagatgctcg accggatgga aggcgtctat catcgccttg tcaaggatcc ggcctgatgc
     6541 ggcaggagga catgatcgga ggacgtacgc cgaggactcg aatgtctctc accgccagta
     6601 cgatcctcaa tcatcacaag atgtggtatt ggaaggaact cggatcaaca ctgtgacggc
     6661 cggtcctctc tcattatcga tttatgtcat cgcctataac gatgagccga acatgcgcgc
     6721 ttgcctcgaa tcggtagccg gttgggctgc tgaactgatc gtcgtcgact ctcacagcac
     6781 cgatcggacg gcggcgatcg cccaggagtt caccgacaag gtgtatcaag tcgatttcac
     6841 gggcttcggc gcgctccgca accaggccgt ggcctactgt tcccatgaat ggattttcag
     6901 tctcgatacc gatgagcgga tgactcccga attgaagcgg gaaatagcac aggtagtcga
     6961 actcccgagc gctgccgatg cctattttgt tccgagaaag aattactttc tgggacgctg
     7021 gatcaagcac tgtggctggt atccggatta tcggcagccg caacttttcc ggaagaaccg
     7081 cttgcgttac cgcgaggaac tcgtgcatga aagctttgac tgtgacggca aggtcggccg
     7141 tctgtcacaa ccagcgctgc aatacccctt tcgggacatt gaccattaca tcgccaagca
     7201 ggatcggtat tccgatctca tggcgcggcg catggttgaa caggggtgcc ggtttgccgc
     7261 acatcagctg gtgacgcatc cactggcggc cttcgccaag atgtatggtc tgcggcgggg
     7321 ctttctcgac ggtatgccgg ggttgattct ttccggactc tatggctatt acacgttcat
     7381 gaagtatgca aagttctggg agcttaccaa agaaagcgga aggtagacgt cagccgtcgc
     7441 atgtcatgaa gatcagtgga ttcacctttt gccgcaatgc cgtgaagtat gattatccga
     7501 tcgtggaatc aatcacatcg gcactgcctc tgtgcgatga attcattgtc aacgtcggcg
     7561 ggtgcgggga cggcaccttg gaggtcattc ggtcgattcg agatccaaag atcaaaatcg
     7621 tcgagtccgt ctgggatgaa tcgctccgac aaggcggcct gatctattcg cagcagacca
     7681 atatcgcact tgcccactgt acgggcgatt gggccttcta catccaggcc gatgaagtga
     7741 ttcatgaaga cgacctgccg gtccttcggc gatcgatgga gcagcatgcc gggaacccca
     7801 gcgtgaaggg gctgctgttc cggtacctcc acttcatcgc tgattactgg tcgacgaatc
     7861 cctggttcta tcacaaggcc gtccgggtga tccggaacaa cggggagatt gagtcgtgtg
     7921 gcgatgcggt cggatttcat ttcaagccga ctcagcaata tttgcaaagc ggtccgaggg
     7981 agtggctcgc ctattcaggc gcaaggatgt ttcattacgg ctgggtgaag gatccgaaaa
     8041 ccatgctcga gaaaaagcgg gaacaggcgt cagtctatca tggcgcgaat cttcctcccg
     8101 ccgccgccaa agagttgacg ggggacgctt tccgattcga ggactacggc attttgaaag
     8161 agtttcgcga cagccacccc gccgtcatga agacgcggat ttcctccgcc cgccggtggg
     8221 gtcgcaggcg aacccgatgg ctgaactggc ggttctaccg cgaagtggca cgcagagggt
     8281 tccgcggata gaccgattcg actgtcatga agcgattgtc ttggtggaga aggccatatt
     8341 tctcgcgtcg cgtgttggag atcggtccgg ggcacaatcc ctttgccggc gtcacgcatg
     8401 tgttggaaag gaatgttgcg gacggacggg aacgcggcgg acatgccttg atcgtgccgg
     8461 ccgctgccca cctgattgtc ggggaagcga cagtgcttcc ttttgccgcc ggttcattcg
     8521 acttcgtcta cgcgtcgcac gtcctggagc atgtagaaga tccggaagcg gcttgccggg
     8581 aaatcatgcg agttgcagca gccggctaca tcgagacacc ttcgccgttc ctcgagcagg
     8641 ggctggccct gaaagacgag acgtctccgg accattggtt tcaccggtgg ttcgtgtttt
     8701 ccctgtcgcc cggccgcctc gtctttgagc gcaagtcggt tgaggacgtg agacaattct
     8761 gttcctgtgc ggatggacag ttcctccggg aattttatga atcgctggac tttcggtacg
     8821 cgcagcattt ctttcgacgg tcggcgaaga ccaccgtgtt ctactggaag gggccctttc
     8881 aggtggaggc gcgcgatcac acggttgatt gccggcaaga gggaagacca tgccgcttcg
     8941 tcgggatgag gcgggcgctt atcgcgaatt gcaacgatct gctgcgcgcg ggtagagttc
     9001 gccgtctccc acagcggttt cccaagtcga gggatgtctt tcgcaagtat ggacaatact
     9061 ctctc
//
LOCUS       DASTQY010000232         6711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_849517, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000232 DASTQY010000000
VERSION     DASTQY010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849517"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFR82_07610"
     CDS             complement(<1..228)
                     /locus_tag="VFR82_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU4501501.1"
                     /translation="MPRVDFYRILGVSREASEDVIKKAYRKLVFEHHPDRNPHSKQAE
                     EKIREINAAYEVIGDAEARRNYDRLNWGDEFA"
     gene            414..1652
                     /locus_tag="VFR82_07615"
     CDS             414..1652
                     /locus_tag="VFR82_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4501502.1"
                     /translation="MRRDQWLVLFVAWLGWVFDAMDATIYAIVLHPALHELLHTSSGR
                     PTAEHIGWYGGIIFSIFLIGWAIGGITFGVLADRFGRTKILIATILMYAVFTGAAALS
                     QEWWHLALYRFLTALGIGGEWAAGAALVAESWPEDKRAKAAGILQSAWAVGFFLAALC
                     NLALKDAYGWRGLFLIGILPAFVALLIRWWVKEPPRWRHAHAQGIVPLSAIFKGDLKR
                     ATLVGSALAFVAVFGLWGSTNWAPTLVRELPEFQGADSAALTRSVSYAIMALNTGAIF
                     GYLAFGPLADRFGRRAVFAFMCIGSFIMLPTTYLLPSHYSGVLLLLPVLGFFNNGIFS
                     GFPIYLPELYPTRLRATGAGFCFNAGRVLASVSPFMTGWLVTTLGTFGRAASTVALIY
                     LAGLAVLWFAPETKGKPLTD"
     gene            1700..2332
                     /locus_tag="VFR82_07620"
     CDS             1700..2332
                     /locus_tag="VFR82_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501503.1"
                     /translation="MDDQHTPRTLSYDEKHAAEAAFSGRPFNPRWSARARVVYDGILN
                     SLPASARWEEALPSSTGFVTESASTEHIEDPPADRGDALPASIPFQKAIDSGALIDVT
                     PTAKQLGLAFSVTVTKPLWDTGIAPIHTLTSEEQAARLRDVLMAFRLRLATQPTLSPV
                     IDFPALLNFPPNTIPQPFPLFALVQPDEQNQAMITLLLPSEMATTIIPMN"
     gene            complement(2316..2852)
                     /locus_tag="VFR82_07625"
     CDS             complement(2316..2852)
                     /locus_tag="VFR82_07625"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEU4501504.1"
                     /translation="MASPLTHALVGGTLAAAWHFPWKLWLVGVASALLPDLDAAGYWL
                     GVPYGSFWGHRGFTHSIVFAVLVSGLFARWIRWSGITVVRVWTYCFVAMLSHGILDAM
                     TDGGLGVAFFSPMDQNRYFLPIRPIHVASMSLHDLLGPHGLDVLASECVWVWIPCAVV
                     MPLLWWFGRTRRPVSSLE"
     gene            2944..3537
                     /locus_tag="VFR82_07630"
     CDS             2944..3537
                     /locus_tag="VFR82_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501505.1"
                     /translation="MLSARIRPASRAAFLLPLTLFVACSSFVYEPAPVVPTTLPLPYS
                     ATFHLAELEAYNVEPGATMVADPRIENKVRGVRQPLSKAKKEWERSIADYVVARKTFT
                     YLSADSQTDLDLSIRLNLYIDPSVNSEFARIYVARIDATLAEGSTKRPLSKYLGFGKA
                     AGEDDDRGPVNLAVQAALNDLFGKIENDSRLRRPGLL"
     gene            complement(3476..4747)
                     /locus_tag="VFR82_07635"
     CDS             complement(3476..4747)
                     /locus_tag="VFR82_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501506.1"
                     /translation="MGLYLVGGVVRDLLLKRSNWDLDLTVEGDGIAFARLVASRYRAG
                     VVLFERFATARLTMSDSTKVDIASTRRESYADPAALPDVRPARLEDDLLRRDFTINAM
                     AIELNGAHWGCLHDPYGGREDIKAKIVRVLHAKSFVDDPTRIFRAIRFAERFGFRLDA
                     TTHRLLKQAAETNIVDQLSGPRLANEIFMLLRERYPERALAALARLQLLRFLHPRLTY
                     SRQARLLMTVLPRGIGWWERQCPGNPIDRSLVWFMALLEGAGSATIAAIAQRLRLSSL
                     QIRTLEWAGTTTGHASDTISGSNLRPSQVYRLLKDMPNEALVLAMSKTLLSHDRVRVG
                     RAGRRLVRFLTRDRQVPMTVDGEDLKQFGLRPGPQFKRILDRLLTERIDGTIKTAVEE
                     RALARRLVAATLRAGQVGANGNRSRSFRTGR"
     gene            5230..5625
                     /locus_tag="VFR82_07640"
     CDS             5230..5625
                     /locus_tag="VFR82_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501507.1"
                     /translation="MNAKRKTILPVVIAMVSILAIAVAPALAKDSKKKAPPSHKSAAA
                     AAASMGHVPQVSEAPKRGHHDEKPGPAWKTVGGTVKDIQDNAYTVEDYEGNQVKLYVG
                     QGTKHIKHKKVGDTVRAEITRGGFANSIQ"
     gene            complement(5725..6477)
                     /locus_tag="VFR82_07645"
     CDS             complement(5725..6477)
                     /locus_tag="VFR82_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEU4501508.1"
                     /translation="MDRTFFRRNLWLAAVWLLSPSVSTAAEPSPVLEVGRFASSHAGA
                     PLTDGWKPLRFKKVPNHTVYELVEDAGAVVVRATSEASASGLIKEIKIDPSAFPVVRW
                     RWKIENLLKHSDVSRKSGDDYPARLYVTFEYDPDKVSFPKKLKYKAGQALLGDIPIAA
                     LNYIWDTKAPIGTVVDNAFTDFAKMIVVESGPAKVGMWVEESRNIYEDYKTAFGEEPP
                     KINGVAIMSDTDNTKERAIAYYGNIQFVPAGR"
     gene            complement(6470..>6711)
                     /locus_tag="VFR82_07650"
     CDS             complement(6470..>6711)
                     /locus_tag="VFR82_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU4501509.1"
                     /translation="GSDKILGATIVAAHAGDLINEFSVIMNAGKGARTIAGTIHPYPT
                     QAEVNKKVVNLWRKAHFTPATKNKLMRLFAWMRRG"
BASE COUNT         1395 a         2012 c         1855 g         1449 t
ORIGIN      
        1 cgcgaattca tcgccccagt tcagccggtc gtagttgcgc cgcgcttccg catcaccgat
       61 gacctcgtag gcggcattga tctcgcgaat cttctcctcg gcctgcttgc tgtgcggatt
      121 gcggtcagga tgatgctcga agaccagttt ccggtaggcc ttcttgatga cgtcttccga
      181 cgcctcccgg gacacgccca gaatccgata gaaatccacg cgcggcatgg gcgcgactat
      241 aacaggatgt cgaaaagagc ttccagcttc accttgactc cacaagggaa gatccttagc
      301 cttgcgcagc ccatgtcccc ttcgtggatt catccgagga cactcagcca acaacgatcg
      361 gtgcagtccc tccccagagg caattggtga gcaggcagtc ctggtggcag ggtgtgcgtc
      421 gagatcaatg gctcgtgttg tttgtcgcat ggctcggctg ggtcttcgat gccatggatg
      481 ccacgatcta tgcaatcgtt ctgcatccgg ccctgcatga attgttacac acctcgagcg
      541 gccgaccgac cgccgaacac atcggctggt acggtgggat tatcttttcc atttttctga
      601 tcggctgggc aatcggcggg atcacgtttg gagtcctggc ggatcgcttt ggacgcacca
      661 agatcctcat agcgacaatc ctcatgtatg ctgtgttcac cggcgcggcc gccctctcac
      721 aggagtggtg gcatctggcg ctctaccggt ttctcacggc actgggaatc ggcggagagt
      781 gggccgcggg agcggccctt gtcgcggaat cctggccgga agacaaacgg gccaaggcgg
      841 caggaatttt acagtccgcc tgggccgtgg gattttttct ggcggctctc tgcaatcttg
      901 ccctgaaaga cgcgtacgga tggcggggtc ttttcctcat cggcatcttg ccggccttcg
      961 tcgccttact gatcaggtgg tgggtgaagg agccaccacg ctggaggcac gcccatgcac
     1021 agggaatcgt cccattgtcg gccattttta aaggggatct gaaacgagcg acgctcgtgg
     1081 gatctgccct ggctttcgtc gcggtgttcg gtctttgggg atcgacgaac tgggccccga
     1141 cgctcgtgcg ggagctgccg gaatttcagg gagcggactc cgcggcactc acacgatcgg
     1201 tcagctatgc cattatggcg ctgaataccg gagcaatctt cggatatctt gccttcggcc
     1261 cattagctga ccgattcgga cgccgagcgg tctttgcgtt catgtgtatc ggcagcttta
     1321 tcatgttgcc cacgacgtat cttctgccgt ctcattacag cggcgtcttg ctgcttttac
     1381 cggttctcgg tttcttcaac aacgggattt ttagcggatt cccgatctac ctgccggagc
     1441 tgtatccgac gcggctccgc gccaccgggg ccggcttttg cttcaacgcg ggcagggtcc
     1501 ttgcctcagt ctcgcccttc atgaccggct ggctggtcac gacgcttggc accttcggcc
     1561 gcgcagccag tactgtggcc ttgatttatc tggccggctt ggccgttctg tggttcgcgc
     1621 cggaaaccaa aggcaaaccg ctgacagact aattgtgcca aacccttgac ttccgccagg
     1681 tccgatttat ttgagtctta tggatgatca acacacccct cgtacgctct cctacgacga
     1741 gaagcatgca gcagaagcgg ccttcagcgg ccgtccgttt aatccacggt ggtccgcccg
     1801 agccagggtc gtctatgacg ggattctcaa ttccctgccg gcctccgcga ggtgggaaga
     1861 agccttgccc tcatcgaccg gctttgtcac ggaatcggcg tcaacggagc atatagaaga
     1921 cccgcctgct gatcggggcg atgctctacc tgcgtccata cctttccaga aggcgattga
     1981 ctcaggggcg ttaatcgatg tcacgccgac cgcaaaacag cttggcttgg ctttctcggt
     2041 aaccgtcacc aagccgctct gggacaccgg tattgcaccc atacataccc tcacgtcgga
     2101 ggagcaggcc gcccgattgc gcgacgtgtt gatggctttt aggcttcgcc tggcgaccca
     2161 acctaccctg tcaccggtga ttgacttccc ggcattgttg aattttccgc ccaacacgat
     2221 accacagcct tttccactgt tcgctctcgt acaaccggac gagcagaatc aagccatgat
     2281 cacgttgttg ctgccaagtg aaatggcgac gacgatcatt ccaatgaact gaccggacgt
     2341 ctagttcgac caaaccacca cagcagtggc atcacgacag cgcagggaat ccatacccat
     2401 acgcattcac tcgccagcac atcgagccca tgagggccta aaagatcgtg cagagacatc
     2461 gaggcgacat ggatcggacg aatcggcaag aagtatcggt tctgatccat cggcgagaag
     2521 aatgccactc ccagcccacc atccgtcata gcatccagaa tgccatggga aagcatggcg
     2581 acaaaacagt aggtccagac tcggacgacg gtgatgccgc tccagcgaat ccatcgcgcg
     2641 aacagcccac tgaccaggac agcaaagacg atggagtggg tgaagcctcg atgaccccaa
     2701 aacgagccgt agggcacgcc gagccaatac cctgcggcat cgagatcggg caaaagggca
     2761 gaagccacgc ctaccagcca cagtttccaa gggaagtgcc aggcggcggc gagagtacca
     2821 ccgaccagtg catgcgtaag gggagaagcc atggcgatcc tagtttattc ggaccccttg
     2881 caaccgcgca taccgagttg tagtgtgatg catcgtcaga ccacgaaaga caagggagca
     2941 agcatgcttt cagcgcggat ccgtcctgcc tctcgcgccg cctttttgct gcccctaacg
     3001 ctgttcgtcg cctgctccag ttttgtctac gagccggccc cagtggttcc aaccacgttg
     3061 ccccttccct attcagccac cttccatctg gcggaactcg aagcctataa tgtggaaccg
     3121 ggcgccacga tggtcgctga tcctcggatc gaaaacaagg tcagaggcgt tcgtcaacca
     3181 ctgagtaagg caaaaaagga atgggaacgg tcgatcgcgg attatgtggt ggcgaggaaa
     3241 acgtttacct acctgtcagc cgatagccag accgatctcg atctgtccat tcggctcaac
     3301 ctgtatatcg acccaagtgt caattccgag ttcgctcgca tctacgtggc ccgtatcgac
     3361 gcgacattgg cggaagggtc caccaaacgt cccctctcga aatatctggg tttcgggaaa
     3421 gctgcaggag aggacgacga ccgcgggccg gtcaatctcg ccgtccaagc cgcgctcaac
     3481 gacctgttcg gaaagatcga gaacgattcc cgtttgcgcc gacctggcct gctctaagcg
     3541 tggcggcaac taggcgccgc gccagtgccc gctcttcgac cgccgtcttg atcgtcccgt
     3601 cgattcgttc ggttagcaac cgatccagaa ttctcttgaa ttgcgggcca ggccggagtc
     3661 cgaactgttt aagatcttcg ccgtcgacgg tcatcggcac ctgccgatcc cttgtaagaa
     3721 atcgcaccaa tcgtctgccg gctcttccca ccctcacccg gtcatgactg agcaacgtct
     3781 tggacatggc caaaaccaac gcttcattcg gcatgtcctt caacagtcga tacacctgag
     3841 agggccgtag gttcgagccg gaaatcgtgt cacttgcgtg gcctgtggtg gttcctgccc
     3901 attcgagggt tcgtatctga agactagata atcgaagccg ttgagcgatt gccgcaattg
     3961 tcgcggaacc ggcaccctcc agcagtgcca tgaaccacac caacgagcga tcaatcggat
     4021 ttcccgggca ctgccgttcc caccagccaa tccctcgggg aagcacagtc ataagaagcc
     4081 gcgcctgcct gctatacgtc agacgggggt gcaggaatcg cagcagctgc aaccgggcca
     4141 acgcggcaag cgctcgctcg ggatagcgtt ccctcaagag catgaagatc tcgttggcca
     4201 ggcggggacc ggaaagctga tcgacgatgt tggtctccgc agcctgcttc aagagacggt
     4261 gagtcgtggc gtccaggcga aagccgaatc gttcggcgaa acgaatggcc cgaaaaatcc
     4321 ttgtcggatc gtctacaaaa cttttggcat gcagcactcg cacgattttg gctttgatat
     4381 cctcccgccc gccgtacgga tcgtgaaggc atccccaatg tgcgccattc aactcgatcg
     4441 ccatggcatt gattgtgaag tcgcggcgaa ggagatcatc ttcgaggcgg gccggacgca
     4501 cgtccggcaa agccgccgga tcggcatagg actctcgacg ggtgctagca atgtccacct
     4561 tggtgctgtc ggacatcgtc aatcgtgcgg tcgcgaagcg ctcgaacagc accacaccgg
     4621 cacggtagcg actggccaca aggcgagcaa atgcgatgcc gtcaccctcg acggtcaggt
     4681 cgagatccca attgctccgt ttgagcaaga gatccctcac gacaccgccg acaagataca
     4741 gacccactcg ttgctcgtcc gccattctcc cgagatcgcg caacaacaga gaggcgtgtc
     4801 gtggcaatcg gcgcaaaaga tattctgcac ccctccgcgg caacatctcg cgcattgtag
     4861 cggtttccta catgtctcgt aacggcctac ctagacaggt tcaaggacgg agttggagca
     4921 cggatcacgt tccagaggga attatgccca gtgatcttac tggcattatt ttctggcgat
     4981 cgtgctgttc gcgttagatg cgcaaccttt tgccctgcca tcagtgttag tcatatcggc
     5041 acagagggtc atatttggac agatttctgc cgtttttttt ccattcgtga cagggtgacg
     5101 gtggcttggt taattgggag ctacaaccta ctgaatttcc acgagccggt agccactcca
     5161 gcgggcacat gccttgctga aagggtcagg caaggctatg acccctatgt tgtcttaagg
     5221 aggtttccca tgaatgcaaa gaggaagaca atactcccag tcgtcatcgc aatggtctcg
     5281 atactggcca tcgccgtcgc accggccctg gccaaagatt cgaaaaagaa agcgccgccc
     5341 tcacacaagt cggccgctgc cgcggcagcg tccatgggac atgtgccgca ggtctcggaa
     5401 gcccccaagc gtggccatca cgatgaaaaa ccagggcctg cctggaagac tgtcgggggc
     5461 accgtcaagg atatccaaga caacgcctat acggtcgagg actatgaagg caaccaggtg
     5521 aagctgtacg ttgggcaggg cacgaagcac atcaaacaca agaaggttgg agacaccgtc
     5581 cgcgctgaga ttacgcgggg cggcttcgcg aattccatcc agtaatctcg ttcgaatccg
     5641 ccacccgctc cccgcgtggt ggtgtctgcg tagaggccga ccctgaacag gtcggccttt
     5701 ttacctctca ggactttctc ttgttcaacg gccggcaggt acaaactgga tgttgccgta
     5761 atacgcgatc gctcgctctt tggtattgtc ggtgtcgctc atgatcgcta cgccgttgat
     5821 cttcggtggt tcttctccaa acgccgtctt gtagtcctcg tagatgttac gcgattcttc
     5881 gacccacatg ccaacctttg ccggcccgct ctccaccacg atcatcttgg cgaaatcggt
     5941 gaaggcattg tcgacgacgg ttccgatcgg cgctttcgtg tcccaaatgt aattgagggc
     6001 tgcgatagga atgtcgccca agagcgcttg tccggctttg tacttgagct tcttgggaaa
     6061 gctgactttg tccgggtcat attcgaacgt cacgtacagc ctggcaggat agtcgtcccc
     6121 ggatttccgg ctcacatcac tgtgcttgag aagattctca atcttccacc gccaacgcac
     6181 gacgggaaat gccgacggat cgatcttgat ctctttgatc agacctgagg ccgacgcctc
     6241 gctcgtcgcc cgtacgacca ccgcccctgc gtcttccacc agctcataga ccgtgtggtt
     6301 ggggactttc ttgaatctga gcggtttcca gccatctgtc agcggcgcac cggcgtgact
     6361 tgaggcaaac cttccaactt cgagcacggg agaaggctcg gccgccgtcg atactgaagg
     6421 ggacaagagc cacactgccg ctaaccagag gttcctccgg aagaatgtcc tatccacgcc
     6481 gcatccatgc gaacagccgc atcagcttgt tcttcgtcgc cggggtgaaa tgtgccttcc
     6541 tccataaatt gacgactttc ttgtttactt cggcttgagt cggataagga tggatggtgc
     6601 cggcgatagt cctcgcgccc ttgcctgcgt tcatgattac cgaaaactca ttgatgaggt
     6661 ctccggcatg ggcagcgacg atcgtcgcac ccaggatctt gtcgctgccc t
//
LOCUS       DASTQY010000233         5294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1458439, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000233 DASTQY010000000
VERSION     DASTQY010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458439"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1100)
                     /locus_tag="VFR82_07655"
     CDS             complement(180..1100)
                     /locus_tag="VFR82_07655"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU4501510.1"
                     /translation="MSLKRKDLLSLAPLSAEEIKLILETADSFKEVTGREIKKVPALR
                     GRTVVNLFFEPSTRTRTSFELAAKRLSADVINFSPSSSSVVKGETLLDTARNIEAMQA
                     DIIVLRHSSAGAAETLARGVKSSVINAGDGWHEHPTQALLDLYTIRERGLSFQGLRVA
                     IVGDVSHSRVARSNIHALAKLGAEVRVVGPPTMIPMGIDKLGAKAFYNLDDALRGVQV
                     IMMLRLQLERQGRALFPTIREYARLYGLTAERVKMAEPGAIVMHPGPINRGVEIAPEV
                     ADSLSSVILEQVANGVAVRMGILYLMSGAN"
     gene            complement(1097..1690)
                     /gene="pyrR"
                     /locus_tag="VFR82_07660"
     CDS             complement(1097..1690)
                     /gene="pyrR"
                     /locus_tag="VFR82_07660"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU4501511.1"
                     /translation="MSEFPIKASMTPNRQQERLVMEAGDMARAMTRIAHEILERNKGV
                     KDLGLVGIRTGGVHLAHRLVRRIQDIEGVAVPIGELDITLYRDDLSLRKEQPVLRKTS
                     VPFDISGKIIVLVDDVLFTGRTIRAAMDGLIDLGRPAEIQLAVMVDRGHRQLPIKATY
                     IGKNIPTSRDENIQVMLEESGEDDRVVILKGVKEIER"
     gene            complement(1856..3640)
                     /locus_tag="VFR82_07665"
     CDS             complement(1856..3640)
                     /locus_tag="VFR82_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501512.1"
                     /translation="MPADPVLTALSTPGHLTFAENLPFREHFEAAGGRVVRQPTGHLV
                     FYRPDGRRILATDPSGHVLHESAWGLNDEGKIALLRARMKLDWGAWVGLKPSGLINET
                     TLNLATKPGWQRLTADDLRAMAAQALHVPIEDIRWFYRDEDLVIGPSGAATIRHRKDA
                     WYVLKEGSFEGARFMSCMGAMRWESIDFLPVVELFKSLLPGTGSAAFEFIRGLYDDQN
                     DGKAPPPPLRYRGIPAYPSEAAFRLFSSCFVPQAPPGKDALSLFMDPTKARQVEWFPS
                     PTPPRRYFDPSAGSCLTMRGGRLEKVTLAGDSAGLPYLNSKGRAFPTCDRAAKIINGQ
                     VVLSDREQDTILSTPLPPGSGDEPVSSPPISPVDWRSVFIQGLPAISPHQAYGAVLLY
                     PQDDATIGELAAQPFVADYLQDLAEQDREIGSLLSRAENVLIENGDAVIATCVPFDRP
                     RNYTVGVHAPAFAQKQAQQLWSTCAQLQRWDWLKRIQFVPVMSRRHRQSSVTTFDVAY
                     YWISSGLNDNRIELAAAIEDLRRALCAGGHAFVTGPGSLMEHWPKVRFEVRWKESVDQ
                     LPTFRMHKTILPQSRLEAGLTVFHVAAT"
     gene            complement(3709..5028)
                     /locus_tag="VFR82_07670"
     CDS             complement(3709..5028)
                     /locus_tag="VFR82_07670"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU4501513.1"
                     /translation="MSEQSGEADRSQDISPEASPATQSIPEYVEELVTRAREAANRLV
                     TLSTSVKNQALLAMADALEAQSERILDANGQDIDAFATRDKMAMADRLRLTDVRIRDM
                     ADGIREVAKLPDPLGEMAKMWTRPNGMQVGRVRVPIGVIGIIYESRPNVTADSATLCL
                     KSGNACVLRGGSEAITSNSAIAAILSETAEKCGIPAGAISFVARPDRELVPLLLKKDR
                     SIDLIIPRGGESLMRIVAEHSTIPVIKHDAGVCHIYVDDDADPVMAERVCLNAKVQRP
                     STCNAMETLLVHQSLARTWLTGFVDKLVAAGVEVRGCQKTCALSSAAKPASDSDYGKE
                     FLDLTLAIKVVKHLDEAMEHIVRYGSRHTEAIVTSDYKKAMRFLREVDASAVLVNAST
                     RLNDGYQFGLGAEIGISTSRIHARGPMGLEELTCSKFIVFGSGQVRE"
BASE COUNT         1123 a         1566 c         1483 g         1122 t
ORIGIN      
        1 gaacgacgag acgtccggtg gcagagaccc tggtcgcgcc cggcggggct ttgacgcccc
       61 gaccgatcgc gacaattttc ccttcgtcga tgaccagatc gccgatggca ttataccggc
      121 cggggtcgat gatgtgtccg ccttcgatca gaagcgccat tatgtatagt ttccttgaac
      181 tagttagccc ctgacatgag atagagaatt cccatcctga cggccacacc gttggcgacc
      241 tgttccaaaa tcacggagga caggctgtcg gcaacctctg gagcgatttc cacaccgcgg
      301 ttaattggtc cgggatgcat cacaatcgcc ccgggttcgg ccattttgac tcgttccgcc
      361 gtcagcccgt acagtcgagc atattcccga atcgtcggga acagcgcacg cccctgacgt
      421 tccaattgca gccggagcat catgatcacc tggacgccgc ggagggcgtc atcgagattg
      481 taaaaggcct tcgcacccaa tttgtcgatg cccataggaa tcatagtcgg aggaccgacg
      541 acccttactt ccgcgccgag ttttgcgagc gcatggatgt ttgagcgggc aacccggcta
      601 tgcgacacgt ccccgacgat ggcaacgcga aggccctgga acgacagtcc ccgctcgcga
      661 atcgtgtaga ggtccagcaa agcctgcgtc ggatgttcgt gccatccatc cccggcgttg
      721 atcaccgaac tcttgacgcc gcgggccaat gtttcggccg cgccagccga ggaatggcgc
      781 agcacgatga tatccgcctg catggcttcg atattacgcg ccgtatccag cagcgtttca
      841 cctttcacga cgctgctcga cgacggagaa aaattgatca cgtcggcgct caaacgcttg
      901 gccgcgagct cgaaggatgt cctcgttcgc gtactcggct cgaaaaagag attgacgacc
      961 gttcggcccc gcagggcggg cactttcttg atttcccgtc cggtcacctc cttaaaagag
     1021 tcggccgttt cgagaatcag tttgatttcc tccgccgaca aaggggctaa gctgaggagg
     1081 tcctttcgtt tcaggctcat cgctctatct ccttcacgcc tttgagaatg acgacccggt
     1141 catcctcacc cgattcttcc agcatgacct gaatgttctc gtcccgtgag gtcggaatat
     1201 tcttaccaat gtaggtcgct ttgatcggca actgccggtg tccccggtca accatgaccg
     1261 ccagctggat ttctgcggga cgccccagat cgatgagtcc gtccatggca gcgcgaatcg
     1321 tccgtcctgt aaacaacacg tcatcgacca gcacgatgat tttcccagaa atgtcgaacg
     1381 gcacagacgt ctttcgaagg accggctgct ctttccgcag ggacaagtcg tcacggtaaa
     1441 gcgtgatgtc caactcgccg atgggaacgg cgacgccctc aatatcttga atccgccgca
     1501 caagccgatg agccaaatgc acgccgcccg tccggatccc gaccagtccc aagtccttca
     1561 cgcccttgtt ccgttcaaga atttcatggg caatcctcgt catggcgcgc gccatgtcgc
     1621 cagcctccat cacgagtcgc tcctgctgcc ggttcggtgt catcgacgcc tttatcggga
     1681 attcggacat aaaaaaacct cctcatcagc gtcgatgaga aggtcgttgg ttgtcgccag
     1741 acagcggcgg cccatatgca ctccttgctc acctcgcagg atgagcatta aaggtttcct
     1801 gatcttactg gggtgcaaca aagactgtca accccgtaag gaatattggc ctggcttacg
     1861 tggctgcaac gtggaagacg gtgagaccgg cttcaagtcg cgactgaggc aagatggttt
     1921 tatgcatgcg aaaggtgggt agctggtcga ccgattcctt ccatcggacc tcgaaccgga
     1981 cctttggcca gtgttccatg agacttcccg gtccggttac aaacgcgtgt cctcctgcac
     2041 aaagtgcccg tcgcaggtcc tcaatggcag cggcgagctc aatgcgattg tcgttgagcc
     2101 cactggaaat ccagtaatag gccacatcaa aagtggtgac ggaagactga cgatgccgtc
     2161 ggctcatgac cggaacaaat tgaatccgct tgagccagtc ccagcgctgc aattgcgcac
     2221 acgtgctcca gagctgttgc gcttgttttt gcgcaaaagc cggtgcgtgg actcccaccg
     2281 tataattccg agggcgatca aatgggacgc aggtggcaat gaccgcatct ccgttttcga
     2341 tcaggacgtt ctccgcacgc gaaagcaggc tgccgatctc tcgatcttgt tcggccaaat
     2401 cttgaaggta atccgcaacg aacggctggg ccgccagctc gccgatcgtg gcgtcgtcct
     2461 gtgggtacag taatacagct ccataggcct gatggggtga gatcgcaggc aggccctgga
     2521 taaacactga tcgccaatcc accggactga tcggcggtga tgaaaccggt tcgtcaccag
     2581 agccgggcgg cagcggcgtg gagagaatcg tgtcctgctc gcgatcgctc agcacgacct
     2641 gtccgttgat gatcttcgcg gctcgatcac aagtggggaa tgctctgccc ttcgagttca
     2701 ggtaaggcaa ccccgcggag tcgccggcga gggtcacctt ttcaagcctg ccaccccgca
     2761 tcgtgaggca ggaaccagcc gagggatcaa agtatcggcg aggcggagtt ggtgacggga
     2821 accactccac ctgacgagct ttcgttgggt ccatgaataa ggaaagggca tccttcccgg
     2881 gtggggcctg aggcacgaaa caactgctga agaggcgaaa ggccgcttca gatggatagg
     2941 cagggattcc acgataccga agcggcgggg gtggggcctt gccatcattt tgatcgtcgt
     3001 aaaggccacg gatgaattcg aacgctgccg agccggtacc cggcaataag gatttgaaca
     3061 gctcgacgac cggaagaaaa tcgatcgact cccagcgcat cgcgcccatg caggacatga
     3121 agcgcgcccc ctcaaaactt ccttctttga ggacatacca tgcatccttg cgatggcgga
     3181 tcgttgcggc cccacttggg ccgatgacga gatcttcgtc ccggtaaaac cagcgtatgt
     3241 cctcgatcgg gacgtgcaac gcttgcgcgg ccatggctct cagatcgtcg gctgtcagcc
     3301 gttgccatcc gggcttggtc gcgagattca aggtggtttc gttgatgaga ccggacggtt
     3361 tgaggccgac ccacgctccc caatccagct tcatccttgc gcgaagaagg gcgatctttc
     3421 cctcatcatt cagcccccat gcgctttcat gcaggacatg accggaagga tcggtcgcaa
     3481 ggatgcgccg tccatccgga cgataaaaga ccagatggcc cgtcggttgc cggaccactc
     3541 gtccgccggc cgcttcgaaa tgttctctga aaggcaggtt ttcggcaaac gtcaagtgac
     3601 ctggcgtcga gagggctgtg agaaccggat cggcgggcat ctaaaaggag tagtgaagat
     3661 tatggtgaac caagaagagg gatgccggcg ggttttttca gcatctctct attcacggac
     3721 ctgcccgctt ccgaacacga tgaacttgga gcaggtcagc tcttccaatc ccatcggtcc
     3781 gcgcgcatgg atacgagagg tactgatgcc gatttctgca cccaagccga actggtatcc
     3841 atcgttgagt ctggtcgacg cattgaccag cacggcgctt gcatccacct cacgcagaaa
     3901 tcgcatggct ttcttatagt cggaagtgac gatggcttcc gtgtgacgag acccgtagcg
     3961 aacaatgtgc tccatggctt catcgagatg cttcacgacc ttgatcgcca acgtcaaatc
     4021 caaaaactcc ttcccatagt cactgtcact tgcaggtttg gctgccgacg ataaggcgca
     4081 ggtcttctgg cagccgcgta cttccacgcc ggctgccacc agtttgtcaa caaaacccgt
     4141 cagccaggtt cttgctagac tttgatggac taacagcgtc tccatggcgt tgcacgtcga
     4201 cggccgctgg accttggcat tgagacacac acgctcggcc atgacgggat cggcatcgtc
     4261 gtccacatag atatggcaca cgcccgcatc gtgcttgatg acagggatcg tcgagtgttc
     4321 ggcgacgatc cgcatgaggg attctccccc tcgtggaata atcagatcga tcgaccggtc
     4381 tttcttcaag agcagcggta cgagctctcg gtccggtctg gccacaaagg agatcgcacc
     4441 ggcaggaata ccgcacttct ctgcagtctc agacagaatc gcggcgatcg cgctgttcga
     4501 tgtaatcgcc tcactcccac cccgcaacac gcaagcattg ccggacttca ggcacaatgt
     4561 cgccgaatca gccgtgacat tgggacgcga ttcataaatg atgccgatca cgccaatggg
     4621 cacgcgaacg cgtccaacct gcatgccgtt gggcctggtc cacatcttcg ccatttcacc
     4681 gagtggatcg ggcaatttgg ccacctctcg gatgccatca gccatgtccc ggattcgtac
     4741 atcggtgaga cgcagacgat cagccatcgc catcttgtcc cgcgtggcaa aggcatcgat
     4801 gtcttgcccg ttcgcgtcca ggatgcgttc cgattgcgct tctaacgcat cggccatggc
     4861 cagtaacgcc tgattcttaa ccgatgtgga caacgtcacc aatcgattgg ccgcttcgcg
     4921 cgcgcgcgtg acgagctcct cgacatactc agggattgat tgcgtagctg gggacgcttc
     4981 tggagaaatg tcttgcgaac gatcggcctc gccggactgc tcagacatga atcggtgctc
     5041 ctcttgtggt cgctgggact ctacaggata cagtggggat ttccgagggg tcaaggcatc
     5101 acgagtgacg cctcgcacca aggtcgttac tgagagagcg tttttcgaaa atacgcgatt
     5161 gtacgagtca atgcttcctc gagagggacc tgcggctccc atttgagcaa tgcgttcgct
     5221 ctgcttatat cgggccgccg gaccttggga tcatcggtgg gcaggggatg atagctgatg
     5281 tcgctgcaag aacc
//
LOCUS       DASTQY010000234         6182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1399075, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000234 DASTQY010000000
VERSION     DASTQY010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399075"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VFR82_07675"
     CDS             <1..1047
                     /locus_tag="VFR82_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU4501514.1"
                     /translation="RTDVEEAKAGEIVAVAGLDQVSIGDTIADAVQPSPLPRVTIDEP
                     TVQMTFSVNNSPFAGREGKFLTSRHLRERLFKELETNVSLRAQETDSPDRFLVAGRGE
                     LHLAVLIEQMRREGYELQVSQPEVILHRDGGNVTEPYEELTIQVPETYQGAVIEEIGK
                     RRGEMRHMKLVHSDAGPSEMHLEYHIPTRGIMGLKNILLAKTRGTVIMHHVFKAYETA
                     NEQDLLVAPHGSLCAFEDGTSTAYALFMTQERGVLFIGPGVEVYRGMVVGENSRDEDL
                     DVNVCKEKHLTNMRASGSDEALVLTPPQEMSLEFALEYIGQDELVEVTPNNLRIRKRL
                     LHPEDRRKAKKSGK"
     gene            1129..1881
                     /locus_tag="VFR82_07680"
     CDS             1129..1881
                     /locus_tag="VFR82_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501515.1"
                     /translation="MRIKKKIPKPSAKRPPLYFIGYRGPAPQKEELIFWYNSQYGGPL
                     TIEHEGGAPESWHIRHGPWTAHVVLPLPSTHVVGLKDQLAWEHDQIGAIAPSVMPPRD
                     MPDTILFAARLARGLALLTQGTAHDLATQQYLNPSDWQDRSLSEFVADDHVLVAQDDQ
                     AKSDMIWCYSLGLSKFGLDELETFLPRGMSDRTAKELLVESIREIIRIGQSPKVGASF
                     DLPSLNRTITIANHRTAAPVGRMLGFREVQAS"
     gene            complement(1893..3395)
                     /locus_tag="VFR82_07685"
     CDS             complement(1893..3395)
                     /locus_tag="VFR82_07685"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501516.1"
                     /translation="MNTRPKTIFHPALGLVLMALLAGCGSENNAQTVRVNLSLVVNSQ
                     HVQHGPVSRFFAWIERWFPGATPAWAQSVSEIATIQVQVSGPGIPAPATSTVPVSNPT
                     SGQEIPVSIQAPVGPNRTITVVASNAANQKIFGGTLAGVTLTAGAPIDLEITLVRLFT
                     VTVQKQGDGSGTVTSTPIGIDCGSTCSSQAGQFEEGTTVSLNPVPAPGSAFVGWSGDC
                     SGTAPCRVAGNATVIAGFNVPTSTDRLTVTLGGDGAGAVTSDSSGISCPGTCAADFAT
                     GTIVTLTATPTNGSTFIGFSGAGCSGPASSCTVAMNGDQAVTATFTAAIPIPMSTLAV
                     SRVGSGSGTVTSTNQAGINCGSTCSASFPTGSLVTLTATPATGSTFIGWSNGCTGTGS
                     CDVTLNADQTIVAQFDLVPQMVTLSVNTSGPGDGTVTSDQGGISCPNACSASFIQGTT
                     VVTLIPTPVGNSIFAGWRGGPCEAFGTGPCMITMDDNRSANARFERFGGG"
     gene            complement(3438..4382)
                     /locus_tag="VFR82_07690"
     CDS             complement(3438..4382)
                     /locus_tag="VFR82_07690"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEU4501517.1"
                     /translation="MNFRALVLSVCGAVSLGYGIAAAADVPDIAGIVLAIQGTVTMQP
                     ATMTAPVKLRPFDEIGPLAILDSQTGSRCKVLYADDSLITVGDNSRLEVTEQTYQAGS
                     DQRAFVARLIRGSVRALVARSFEGDNSTFVIDAGTSAVTARGTYFVTWLNGPSARKGK
                     GDEEEGLEMEGATGVANIGRNGNVAFTSGGATVLVLPGQFAIAMPGAPPSMPASIQTH
                     IVPVATAISGTMLPDVPKQESPREALAEAGIIGGGTRGQTLAGPVMAKSGSLGGQAPS
                     GSYMLPDWPFPVTPVTPPAVASGAAISNTTVNLTIRLP"
     gene            complement(4391..6034)
                     /locus_tag="VFR82_07695"
     CDS             complement(4391..6034)
                     /locus_tag="VFR82_07695"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4501518.1"
                     /translation="MSKASSADYDRSASEVNRILSRYPEVIRNLGCCLFLLLIGALIL
                     LGAAEAAEDQREFDFDLSKGIYFYNQARYGEAERHLRDAHNARPTDPTAAYYLGLNAI
                     KLQRYTEAEERFREVLRRKPDDARARMGLGMALYHQNRYPEASAELSVAERTIKNDPL
                     LYYYAGFSAARQQDDELASDKFLKAGKLDPELANNAHYLRGAGLYSQGDYKQATAEFR
                     SAIEGAPAAAAPPSKPAVAPSTQQPSKRWSGNYGLSLQYDSNVVLLPGGSTLPGGISH
                     KDDFVTALTGNVEYRFLQTDTWTIGAGGGLFTNFHARLSDFNVLDFAPTLYVQRQLGS
                     AQLRFQYTLDYVTVGGDAYLLSNALQSTLTIPESERTYTQAFFRYQNKDFKTFRDDEL
                     GVPVNQTRDANNFMLGALQYWRFAEDRGYLRGGYTFDTDVTGGGDTPTTLGLPSKADW
                     SYTGHRLSVGAAYQPLTATTLLLGLDYYRQNYNNPNSFSSDGAIRKDNVYQLTGTVVR
                     DLQSWLWLAFQYSLTRDQSNVEVFDYTRHVISLTLGGAF"
BASE COUNT         1369 a         1801 c         1734 g         1278 t
ORIGIN      
        1 cgcacggatg ttgaagaagc caaagccggc gagattgttg cggtcgcggg attggaccaa
       61 gtgagcatcg gggacacgat tgcggatgcc gtacaaccct caccgctgcc aagggtcacc
      121 atcgacgagc ccaccgttca aatgactttt tcggtgaaca acagcccgtt cgccggccgt
      181 gaaggcaaat tcttgacgtc ccgacacctc cgcgagcgcc tcttcaaaga acttgaaacg
      241 aacgtctctc tccgcgcaca ggaaacggac agtccggatc gattcctcgt ggcgggacgc
      301 ggtgaattgc acctcgcggt cttgattgag caaatgcggc gtgaaggcta cgaactgcaa
      361 gtctcccaac cggaagtcat tctgcatcgc gacggcggca acgtcaccga gccgtacgaa
      421 gaactcacga ttcaagtgcc ggagacgtat caaggggccg tcatcgagga gatcggcaag
      481 cgccgcggag aaatgcggca tatgaagctg gtgcattctg acgccggccc gagtgaaatg
      541 catcttgagt atcatattcc tacacggggg atcatgggcc tgaagaacat ccttctcgcc
      601 aagacccgtg gaacggtgat catgcatcac gtcttcaagg cctatgaaac ggccaacgaa
      661 caggatttgc ttgtcgcgcc ccatggctcg ctctgtgcgt tcgaggacgg cacgagcacc
      721 gcctatgcgt tgttcatgac gcaggagcgc ggcgtcctgt ttatcgggcc aggcgtcgaa
      781 gtctatcggg gcatggtcgt cggggaaaac agccgggacg aagatctgga cgtcaatgtt
      841 tgcaaagaaa agcacctcac gaacatgcgc gcctccggtt cagacgaggc cttggtgctg
      901 actccaccgc aggaaatgtc tttggaattt gccctcgagt atatcgggca ggatgagcta
      961 gtcgaagtca cgccgaacaa tctgcgtatt cgtaaacggc ttctccatcc ggaagatcgg
     1021 cgcaaagcta aaaaatcggg gaagtaaccg tcgtgccagc ataacctgag atccgtgctt
     1081 tcctagcttc gattaccgtt cagcaaaagc accagcgccg cccttccaat gcgtatcaag
     1141 aagaaaatcc ctaagccctc tgccaagcga ccgccattgt atttcatcgg ctatcgcggg
     1201 ccggcgccgc aaaaagagga gctcatattc tggtacaaca gccaatatgg tgggcccttg
     1261 acgatcgagc acgaaggcgg ggcgcctgag tcctggcata tcagacacgg cccttggacc
     1321 gcgcatgtcg tccttccgct cccctcaacg cacgtggtcg gcctgaaaga ccaattggca
     1381 tgggagcatg atcagatcgg cgccattgcc ccgtccgtta tgccaccacg agacatgccg
     1441 gataccatct tgttcgcggc gcgtctcgcc agaggcctgg cactcctcac acagggcacc
     1501 gctcatgacc tggcgaccca gcagtatctc aatccttccg attggcagga tcgtagtctc
     1561 agcgaattcg tcgccgatga tcacgtcctt gtcgcacagg acgatcaagc gaagtcggat
     1621 atgatctggt gctacagcct cggcctctcc aaatttggcc ttgacgaatt agaaacgttc
     1681 cttcctcgag gaatgtccga tcgaacggcc aaggaacttc tcgtcgagtc gatccgggaa
     1741 ataatccgaa tcggtcaatc ccccaaggtt ggcgcatcat ttgacctgcc ttctctgaac
     1801 cgcaccatta cgatcgcaaa ccaccggacc gccgctcctg taggacgcat gctgggtttt
     1861 cgcgaagtgc aggcttcttg aagacgcagt tctcaaccac ccccaaatcg ttcgaatcgg
     1921 gcattcgccg accgattatc atccatagtg atcatgcatg gccccgttcc aaatgcttca
     1981 caaggacctc cacgccatcc cgcaaaaatc gagtttccga ccggtgttgg aatcaaagtc
     2041 acgacagtcg tcccttggat gaaagaagcc gaacaggcgt taggacaaga aatgccgccc
     2101 tgatcgctcg ttaccgtacc gtcgcctggt ccggatgtat tcacagacaa tgtaaccatc
     2161 tgaggcacga gatcgaattg cgcgacgata gtttgatcgg cgttcagcgt cacgtcgcag
     2221 gagccggtcc cagtacatcc attgctccag ccgatgaagg tcgagcccgt tgctggggtc
     2281 gcagtcaatg tgaccaggct tcccgtgggg aaactggcgc tgcaggtgct tccgcaatta
     2341 atgcccgctt ggttcgtgct cgtcacggtc cccgatcccg atccaaccct gctcactgca
     2401 agagtagaca tgggaattgg gatagctgcc gtaaaggtcg ccgtgactgc ctggtcgccg
     2461 ttcatagcga cggtacacga cgaggcgggt ccgctacagc cggcgccact aaatccaatg
     2521 aatgtggatc cattagtggg tgtggcggtg agtgtcacga ttgtcccggt ggcaaagtcg
     2581 gcagcgcagg tacccgggca ggaaataccg ctggagtcac tagtgacggc gcctgccccg
     2641 tccccaccca gggtcaccgt cagccgatcg gtggaagtcg ggacattgaa gccggctatc
     2701 acagtagcgt tccccgctac gcggcagggt gccgtgccgg aacaatcgcc actccaaccg
     2761 acaaacgcag aaccaggtgc cggaaccgga ttcagagaca ccgtcgttcc ctcttcaaac
     2821 tgaccggcct gactggaaca ggtcgatcca caatcgatcc ctatgggcgt ggaggtcaca
     2881 gttccgctgc catctccctg cttttgcacg gtgacggtaa agagtcgaac gagggttatt
     2941 tccaaatcga tcggcgcgcc cgcggttagg gtcacaccgg ccagcgtgcc tccaaagatt
     3001 ttctggtttg ccgcattgga ggccacgacc gtgatcgtcc ggttgggccc gaccggtgct
     3061 tggatgctca caggaatttc ttgaccactt gtgggattgg acacaggaac ggtcgaagtc
     3121 gccggagccg gtatccctgg tccagaaacc tggacttgga tcgtggcgat ctcagatacg
     3181 ctttgcgccc acgcgggcgt cgcaccaggg aaccagcgct cgatccatgc aaaaaatcgg
     3241 gagaccggcc cgtgctgtac atgctgggaa ttgacgacta aagacaaatt cacgcgtaca
     3301 gtctgagcat tattctcgga tccgcagccc gcgagaagtg ccatgagtac gagacccagt
     3361 gccgggtgga aaatggtttt gggtctggta ttcattgaac cacctcaaaa tcgcccaaaa
     3421 tggctccggt tgtaaggcta tgggagtctt atcgtcaaat tcaccgtcgt gttactgatc
     3481 gctgcaccag aggcaaccgc gggaggggtc accggcgtca cggggaaggg ccaatccggc
     3541 agcatataac ttcccgatgg agcctggcct ccaagcgacc cactcttcgc catgacggga
     3601 cccgccagtg tctgccctct ggtgccacca cctataatcc ctgcctccgc caatgcctcc
     3661 cggggcgact cctgcttcgg cacatcgggc agcatggtcc ctgagatcgc cgtcgcaacc
     3721 ggaacgatat gggtctggat ggaggctggc atactggggg gcgccccggg catggcaata
     3781 gcgaattggc cggggagaac caagaccgtg gcacccccgg aggtgaaggc cacattgccg
     3841 tttcgcccga tattcgccac tccggtggct ccttccattt ccaacccttc ttcctcatca
     3901 cctttgccct tccgcgctga aggcccattt aaccaggtca cgaaataggt ccctctcgcg
     3961 gtgacggcag atgtgcccgc atcgatcaca aaggtcgagt tgtctccttc gaacgagcgt
     4021 gcgaccagcg cgcggaccga tcctcgaatc aggcgggcga cgaatgcccg ttgatcgctg
     4081 cccgcctggt aggtctgttc cgtcacttcc agacgactgt tgtcaccgac cgtgatcagg
     4141 ctgtcatcgg catagagcac cttgcagcgt gagccggtct ggctatctaa aatggcaagc
     4201 ggcccgattt cgtcgaatgg acggagcttc actggtgccg tcatcgtcgc cggctgcatt
     4261 gtcacggtcc cctggatggc cagtacaatg cctgcaatat cggggacatc ggcagcagcg
     4321 gctattccat aacccagcga cacggcacca catacggata ggacgagggc acgaaagttc
     4381 atgatgcctc ctaaaatgcg cctccgagcg tgagtgaaat gacatgcctc gtgtaatcga
     4441 atacttcgac gttcgattga tcccgtgtca gcgaatattg aaaagccagc cacagccagg
     4501 attgcaggtc gcgaacgacc gttccggtta gctgatagac gttgtctttc cggatggccc
     4561 catctgaaga gaaggagttt ggattattgt aattctgacg gtagtaatcc aaccccagca
     4621 gcaacgttgt ggccgtcaat ggttggtatg cagctcccac cgacaggcgg tgaccggtat
     4681 aggaccaatc ggctttactc ggcaggccta gagtcgtcgg cgtatcaccg ccgcccgtga
     4741 cgtcggtatc aaaagtgtag ccccctcgca gataaccacg gtcttcggcg aagcgccaat
     4801 attgcaacgc gccgagcatg aagttgttcg catcgcgcgt ctgattcaca ggcacgccca
     4861 gttcatcatc tcggaacgtt ttaaagtcct tgttctggta gcggaaaaat gcctgcgtat
     4921 acgtgcgctc gctttccgga atagtcagcg tggactggag cgcgttcgag aggagatagg
     4981 cgtcgccgcc caccgtcaca taatccaacg tgtactggaa ccggagctgg gccgagccca
     5041 gttgccgctg gacatagagg gtcggagcaa agtccagaac gttgaaatcg ctcaggcggg
     5101 cgtgaaagtt tgtaaataat ccccctccgg caccgatcgt ccatgtatcg gtttgcagaa
     5161 accgatactc aacatttccc gtcaatgccg tgacaaagtc gtccttgtgg gagattcctc
     5221 ctggcagcgt ggaaccacct ggaagcagga cgacattgga atcatattgg aggctcagtc
     5281 cgtaattgcc gctccatcgc ttcgatggct gttgggttga tggggcaacc gccggctttg
     5341 acggcggtgc ggcggcggca ggagcaccct cgatcgctga ccggaattcc gccgtcgcct
     5401 gcttatagtc accctgtgaa tagagtcccg ctccgcggag gtaatgcgca ttattggcca
     5461 actccggatc aagctttcct gcttttaaga atttgtctga ggcgagttcg tcgtcttgct
     5521 gacgcgctgc ggaaaaccct gcgtaataat agagaagcgg atcatttttg atcgtgcgct
     5581 cggcgacact cagttcggcc gaagcttcgg ggtagcggtt ttggtgatag agagccatgc
     5641 ccaaccccat gcgggcgcgg gcatcgtccg gctttcgccg cagtacttcc ctgaaccgct
     5701 cttctgcctc cgtataccgc tgtagtttga tggcattcag tccaaggtag tacgctgccg
     5761 ttggatctgt tggcctggca ttatgggcgt cgcggagatg ccgctcagcc tccccatacc
     5821 gggcttgatt gtaaaagtag atccctttgg agagatcgaa atcaaactca cgctgatctt
     5881 ccgccgcctc cgcggcccca agcagaatga gtgctccgat caacaaaagg aacaggcagc
     5941 atcctagatt ccgaatgacc tcaggatatc gtgacaagat acgattcact tcggatgcac
     6001 tccggtcgta gtcggcggac gaggccttac tcaagaatcg gacctatacc ggaatgagaa
     6061 aagaaggaac gattacaagc ctttgaagtt gattgcatga agcgcccagg acaatctgtc
     6121 ccgtgggcgt cgacttgggg acatcctgtc cctcaaacaa gggtgacgta aggctccaaa
     6181 ct
//
LOCUS       DASTQY010000235         6124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_488071, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000235 DASTQY010000000
VERSION     DASTQY010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488071"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2133
                     /locus_tag="VFR82_07700"
     CDS             <1..2133
                     /locus_tag="VFR82_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEU4501519.1"
                     /translation="IMRGLAEVGLTTREACANTVRNVTGCHLAGVCQGEVFDITPYAK
                     TVAYHLLRNPLNQSLPRKFKIAFSGCKQDCALTPIHDIGLLAVKAEDGTIGFRIVVGG
                     GLGSAPRIAQVLREFTPMDEVIPTIEAVIKVFDTLGNRKNRNKARMKFVIEKLGFAEF
                     KRRWEEAYLSMGHARPTQQPIRLLSHQDEPVSLIMPTSNGVKSGVAQTNGHPNGDHQE
                     TPFDMWKRTNVVRQKQAGYTAAVIKLFMGDLTASQMMHVADFAERYSNGNLRTTINQN
                     LIIRWIPESRLTDLYHDLASQGLSDPGAELVEDIIACPGTDTCGLGITSSKGLARALA
                     EVFPAGRVPDDLTDVSVKISGCHNSCAQHHIATIGLHGVGKRIGEHTAPFYELHLGGK
                     VNGTAKIGQMTVKLPAKAVSAAITHLIDVYRRDRQTGEGLPAFIDRVGKNALKDELIP
                     YTIVPPFAEDPTFYYDWEAEEEFVLEDLGPGECAGGALEMIENGILEADQELYQAKLL
                     MDNHQYSVSVNKSYRAVLAAAKALLVTEGLDPSTDADTFREFDGRIAQNGVVPAVYRD
                     LVKKVGDLGPKDTTAESAQEKMAFAKGFVDACRAATEQMGKDLKLTTDKATAASTVAE
                     PEAATQVPTGAPVYDLRGVACPLNYVKTKLKLEMMDAGEKLEVWLDAGEPIKNVPLSL
                     KNDGHLVHLQEALEPEAAHYRILVEKVE"
     gene            2185..2895
                     /locus_tag="VFR82_07705"
     CDS             2185..2895
                     /locus_tag="VFR82_07705"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEU4501520.1"
                     /translation="MHTKQPTGDMLAWAESLEGNQPQEVLAAAIKRYGPRIVLACSFG
                     AEDVVLVDMMHRIDPSVGLFYLDTDFLFPETYATRDRIIERYQLKPAQVHQVTSLLTP
                     ERQAEQYGPALWTSDPDQCCKLRKVEPLTRVLSGFDAWITGIRREQAPTRAHAKPIEW
                     DHIFQLVKVNPLVRWTWADVWTYIKVHEVPYNELHDRNYPSIGCIQCTKPVMPGDDPR
                     SGRWEGREKTECGLHKTS"
     gene            2901..3947
                     /gene="trpD"
                     /locus_tag="VFR82_07710"
     CDS             2901..3947
                     /gene="trpD"
                     /locus_tag="VFR82_07710"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU4501521.1"
                     /translation="MQELIAKIAKGPKASKDLTWDEAKRAIMTLIEGDATPAQAGAFL
                     IAMRFKMESVTELAAFTAATRSYVAPLPIPKELGVVDLPSYAGKQDTFHALAGAAIVA
                     AAAGAAILMHGYDGVPGRPGNAALLKALGIPTDLESKSVAALVSQQGFAYLDIALYHP
                     PLYRFLEMRQELGVRNVFHPVARLLNPARAASQVIGLTHPPHFEKTAEAVRMLGMPRA
                     LVIRGVEGDPELSIAMATKVLELRDERIAPLTLAPKDAGLPLATSRDMAGFPNEHREK
                     EADLLRRILHNRISGGQRDWVLLNAGLLLYAAGKGASLGEGVSLARRTLEDGTAARKL
                     DSLSHATMAVAQQT"
     gene            3965..4762
                     /gene="cysD"
                     /locus_tag="VFR82_07715"
     CDS             3965..4762
                     /gene="cysD"
                     /locus_tag="VFR82_07715"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEU4501522.1"
                     /translation="MKHLRQLEDQSVYILREAYKHFDNLAMLWSMGKDSTVLLWLARK
                     AFFGHVPFPLLHVDTSFKIPAMIEYRDRLAREWHLNLVVGQNKDALAAGMNHTLGRVT
                     CCTALKTNGLKQLLEQKGYTGVILGVRADEEGTRAKERYFSPRDKHGDWDFRDQPPEL
                     WDQFKTTFPPGTHIRIHPLLDWTEINIWEYIKLENIPFIDLYLNKGDGTRYRSLGCAP
                     CTMPIKSTAKTVDEIIEELRHTTVAERSGRAQDEGRGMELLRKDGYM"
     gene            4827..>6124
                     /locus_tag="VFR82_07720"
     CDS             4827..>6124
                     /locus_tag="VFR82_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU4501523.1"
                     /translation="MSTAKSSENLNIVIVGHVDHGKSTLLGRLYADTGSLPDGKLEKV
                     QAICRQQGKEFEYAFLFDAFLEEQEQGITIDTARTFFMWRGRQYIIIDAPGHKEFLKN
                     MISGAARAEAALLLIDALEGVKEQSKKHGYLLSLLGVRQFAVVVNKMDLVGYRQDVFE
                     GIEKEYREFLAQFGAVPERIIPVSAKLGDNIANRSGTMPWYQGPTVLDTLSLFKKETA
                     RSEQPLRFPVQDVYKFDARRIIAGRITAGRLKIGDHLVFSPSNKRANIRSIEAFNVEP
                     VLTGAEAGQSVGITLDEQIFVERGEVATHQEQLPLVSTAFKANLFWLGKQPLEQGRRY
                     LLRVATREVECEVAAIHRIIDTMDLNQQSGSNKVARNQVGELTLRTKAPVAFDLSASF
                     EATGRFVLVDDYDIAGGGIVTEMIRDDQEFLREEARRRDFA"
BASE COUNT         1417 a         1632 c         1828 g         1247 t
ORIGIN      
        1 atcatgcggg gtctggctga agttggtctg acgactagag aagcctgtgc caacacagtc
       61 cgaaacgtca ctggctgcca cttggcaggg gtctgccagg gcgaagtgtt tgatattacc
      121 ccctatgcca agacggtggc ctatcatctc ctgcggaacc ctcttaatca gagcctcccc
      181 cgcaagttca aaattgcctt ttccgggtgc aaacaggact gcgctctcac tccaattcac
      241 gatatcggcc tgcttgccgt gaaagccgag gacggcacga tcggtttccg catagttgtc
      301 ggtggcggac tgggttcagc gccccgcatc gcgcaggtgc tgcgtgaatt taccccgatg
      361 gacgaagtga tcccgacgat cgaagccgtc atcaaggtgt tcgacaccct gggcaacaga
      421 aagaatcgca acaaggcgcg aatgaagttc gtcattgaaa agctgggatt cgcggaattc
      481 aagaggcgtt gggaagaggc gtatctctca atgggccatg cgaggccgac gcaacagccg
      541 atcaggttgc tgtcgcatca ggatgagcct gtttcgttga ttatgcccac gtccaatggg
      601 gtgaagtccg gcgtcgccca gaccaacggt catccgaacg gcgaccacca ggagacgccc
      661 tttgacatgt ggaagcggac caacgttgtg cgccagaagc aggccggcta tacggcggct
      721 gtcattaaat tgttcatggg agacctgacc gcaagtcaaa tgatgcatgt ggcagacttc
      781 gccgagcgct attcaaacgg gaacctccgg acaacaatca atcagaacct gattatccgg
      841 tggattcccg aatctcgttt gaccgatctt tatcacgacc tcgcttcaca ggggctgtcg
      901 gatcccggcg ccgaactcgt cgaagacatt atcgcctgcc ccggcaccga tacctgcggc
      961 cttggtatta cctcgtccaa ggggctggca agggccctgg cggaagtgtt tcccgccggg
     1021 cgtgtgcccg atgaccttac ggacgtgagc gtcaagatca gcggatgcca caactcgtgc
     1081 gcacagcacc atatcgcgac aatcggcttg cacggtgtcg ggaagcgcat cggtgagcat
     1141 acggcgccgt tctacgagct tcatcttggc ggtaaggtga acggcactgc caaaatcggt
     1201 cagatgacgg tcaagctgcc tgcgaaagcc gtatcggcgg ccattacgca ccttatcgac
     1261 gtctataggc gcgatcgtca aaccggcgaa gggctgccag ccttcattga tcgagtgggg
     1321 aaaaatgcgc tgaaagatga actgattcct tatacgattg tgccgccgtt tgccgaggac
     1381 cccacgtttt attacgactg ggaggcggaa gaagagtttg tcctcgaaga ccttggtccc
     1441 ggtgaatgtg ccggcggcgc cctggagatg atcgagaatg gaatcttgga agccgatcaa
     1501 gagctgtatc aggcaaagct gttgatggac aaccatcaat actccgtctc ggtgaacaag
     1561 tcgtatcgcg cggtcctcgc ggcagcgaaa gcgctgttgg tgacggaggg tctcgatccc
     1621 tcgacggacg ccgatacgtt ccgtgagttc gacggccgga tcgctcaaaa cggcgtcgtg
     1681 ccggccgtct atcgtgacct cgtcaagaag gtgggggact tggggccgaa agatacgacg
     1741 gcagaatcgg ctcaagagaa aatggccttt gcaaaaggct ttgttgatgc gtgccgggcc
     1801 gcgacagagc agatggggaa ggatctcaag ctcacaactg acaaagcgac ggcggcctct
     1861 acggtcgctg agccggaagc ggccacacag gttcccaccg gcgctccggt ctatgatctc
     1921 cggggtgtcg cgtgccccct caattatgtg aaaacgaaat tgaaactcga gatgatggat
     1981 gctggtgaaa aattggaagt atggttggac gccggcgagc cgatcaagaa tgtgccactc
     2041 agtttgaaga atgacggaca tctcgtgcac ttacaggagg cgctggaacc ggaagcggcg
     2101 cactaccgaa tcctggtcga aaaagtggaa tgagttgagt gcgcatctca gtgccgcatg
     2161 gatagggtgg aaagaaaagg gccaatgcac acaaagcaac caactggcga catgctggct
     2221 tgggccgagt cgctcgaggg aaaccaacca caagaggtgc tcgccgccgc catcaagcgc
     2281 tatggcccgc gaattgtgct cgcctgcagc ttcggtgcgg aggacgtggt cttagtcgat
     2341 atgatgcatc ggattgaccc gtcagtgggg ctgttctatc tcgatacgga tttcctcttc
     2401 cccgagacgt acgcgacgcg cgaccgaatc atcgagcgat accagcttaa accggcccaa
     2461 gtgcatcaag tgacgtctct gctgacaccg gaacggcagg cggaacagta tggccctgcc
     2521 ctgtggacga gcgacccgga ccagtgctgc aagttgagaa aagtcgaacc cttgacgcgg
     2581 gtgctgagtg gattcgatgc gtggattacc ggcatccgac gggaacaagc gccgaccaga
     2641 gcccatgcca agccaatcga gtgggatcac atttttcaac tcgtcaaggt gaatccactc
     2701 gtccggtgga cgtgggccga cgtctggacc tatatcaaag tgcacgaagt gccctataac
     2761 gaattgcatg accgcaatta tccaagcatc ggctgtattc agtgcacgaa gccggtcatg
     2821 ccaggcgatg atccacggtc cggccggtgg gaaggtcgcg agaagaccga atgcggcctt
     2881 cataagacat catgatgggc atgcaagaac tcattgcgaa aatcgccaag ggacccaagg
     2941 cttccaagga tctcacgtgg gacgaagcca agcgggccat catgaccttg atagagggcg
     3001 acgccacgcc cgcgcaggcc ggagcatttc tcattgccat gcgtttcaag atggagtccg
     3061 tgacagagtt ggccgccttc actgccgcga cgcgctccta tgtggcgccg cttccaatac
     3121 ccaaggaact cggtgtggtt gatctgccgt cttatgccgg taagcaggac accttccatg
     3181 cactggccgg cgccgcgatt gtggcagctg cggccggtgc tgccattctc atgcatggat
     3241 acgatggcgt tccaggtcgt ccgggaaatg ccgctctgct gaaggccttg ggaataccca
     3301 ctgatcttga gtccaaatcc gtggccgcac ttgtatcgca acaggggttc gcctacctcg
     3361 acattgcgct ctatcatcct ccgctctacc ggtttctgga aatgaggcag gaactggggg
     3421 tacggaacgt gttccatcct gtcgcgcggc tcttgaatcc tgcacgcgcc gcctcacagg
     3481 ttatcggcct tactcatcca cctcatttcg aaaagacggc cgaggccgtt cggatgttgg
     3541 gcatgccgag agctcttgtg atacgcggcg tcgagggcga tcccgagctg tcgatcgcca
     3601 tggcgaccaa ggtgttggaa ttgcgcgacg aacggatcgc accattgact ttagcgccca
     3661 aggacgccgg actgccattg gcaacgtcca gagacatggc tggctttccc aacgaacatc
     3721 gagaaaagga ggcggatctg ctcagacgga ttcttcataa ccggatttcc ggcggccagc
     3781 gggactgggt cctgctcaac gccggcctgt tgctctatgc ggcagggaaa ggggcatcgt
     3841 taggagaagg tgtatcgttg gcgcggcgga cgcttgagga tggaactgcc gcgagaaaac
     3901 tggatagcct gagtcacgca accatggctg ttgctcaaca gacttagagg taagaggttc
     3961 atcagtgaaa catctgcgcc aattggaaga ccagagcgtg tacattttga gggaagccta
     4021 caaacatttc gacaatctgg ccatgctgtg gtcgatgggg aaagattcga cggtgctgct
     4081 atggctggct cgaaaggcgt ttttcgggca tgtaccattc ccgctgctgc atgtagacac
     4141 cagtttcaaa attcccgcta tgatcgaata ccgggacagg ctcgcaaggg aatggcatct
     4201 gaatctcgtc gtcggccaaa acaaagacgc tctggccgcc ggtatgaacc atacattggg
     4261 ccgcgtcacc tgttgcacgg cgttgaaaac caacgggctc aaacaattgc tggaacagaa
     4321 gggctacacc ggagtcattt tgggggttcg ggcggatgag gaaggcacga gggcgaaaga
     4381 acgttatttt tccccccgcg ataagcacgg cgattgggat tttcgcgacc aacctccgga
     4441 actctgggat caattcaaga ccacgttccc tcccgggaca cacatcagga tccatccgtt
     4501 gctggactgg acggaaatta atatctggga atacatcaag ctcgagaata ttcccttcat
     4561 cgacctctac ctgaacaagg gagatgggac tcgttaccgc agtctgggct gtgctccctg
     4621 caccatgccc atcaaatcca cggcgaaaac cgtcgatgag attatcgaag agctgcgtca
     4681 tacgactgtg gccgagcgct cagggcgtgc gcaggacgaa ggtcgtggga tggagttgtt
     4741 acgaaaagat ggatacatgt agcgccaaag tgttctgccg ctgctggtta tcggttggga
     4801 ttggctgatc aggttcgagg agcattatga gtacggcgaa atcgtccgag aatctcaata
     4861 tcgttatcgt ggggcacgtt gaccacggca agtcgacgct gctcggacgg ctctacgccg
     4921 ataccggctc tctgccggac ggaaaattgg aaaaggtaca agccatctgc cggcagcaag
     4981 gaaaggaatt cgaatatgcc tttttgttcg atgcctttct ggaagagcag gagcaaggca
     5041 tcacgattga caccgcgcgg acgttcttca tgtggagagg acggcaatac atcattatcg
     5101 atgcaccagg gcacaaagaa tttctcaaaa atatgatctc cggcgcggca agggcggagg
     5161 ctgcgctgct gctgatcgac gcgttggagg gagtaaaaga gcaatccaaa aagcatggct
     5221 atctcttgtc tctcttgggc gtccggcaat ttgcagtcgt ggtcaacaag atggatctcg
     5281 ttggataccg gcaggatgtc ttcgagggca tcgagaagga gtatcgcgaa tttctggctc
     5341 agttcggtgc ggtgccggag cgtattatcc ccgtgagtgc aaaattgggt gacaacatcg
     5401 ccaaccggag cggcaccatg ccctggtatc aggggccaac ggtattagac acgcttagcc
     5461 tcttcaagaa ggagacggcg cgatcggagc agccgttgcg gtttccggtg caggacgtct
     5521 acaaattcga tgcccggcgg atcatcgccg gccgcattac agccggacgg ctcaagatcg
     5581 gcgaccatct cgtattttca ccgtcaaata aacgagccaa catccgctcg atcgaagcgt
     5641 ttaatgtcga accggttctc accggagccg aggcgggaca gtctgttggc atcactcttg
     5701 acgaacagat atttgtcgag cgaggagaag ttgccacgca tcaggagcag ttgccgctgg
     5761 tatcgacggc atttaaggcc aacctctttt ggctggggaa gcagccgctg gaacaaggtc
     5821 gcaggtatct cctgcgagtg gccacgaggg aagtcgagtg cgaggtggcg gccatccacc
     5881 ggatcatcga caccatggac ttgaatcagc agtcaggcag caacaaggta gctcgtaacc
     5941 aagtcggaga attgaccttg cgaaccaagg ctccggtcgc attcgacttg tctgcctcat
     6001 tcgaagcgac aggtcgtttt gttctggttg acgattatga tattgccggc ggcgggatcg
     6061 tgacggagat gatcagagac gatcaggaat ttctccggga agaggcgcgc cggcgagact
     6121 tcgc
//
LOCUS       DASTQY010000236        11171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1770202, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000236 DASTQY010000000
VERSION     DASTQY010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11171
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770202"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..882)
                     /locus_tag="VFR82_07725"
     CDS             complement(4..882)
                     /locus_tag="VFR82_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU4501524.1"
                     /translation="MDSSTLSLPFRIPNRKFQALSAYVSPLMAAGFVLATAFPAFVQA
                     EVSGNLVIVGNGPELTTIESLSRAFEKANPRAYVDVIWDDHSKPVEMVKAGDAHIAVT
                     GSEDSGLAATQIAWDGIGILVHLSNFTKEVTKQQVADMFSGKVKEWSELGGPETKILL
                     IDRPKNQNIRDAFERQLGIDGKIPDNAKIIAKDDKVIKTVVGTLPPMSAVAYISLTQG
                     LSVVTSGVAVRLLPVDRVEPEIPTVKDGRYPLRRPVLLLSKKDPNPLVEAFGQFALSP
                     AGRQIIAESYIPFERK"
     gene            complement(892..1221)
                     /locus_tag="VFR82_07730"
     CDS             complement(892..1221)
                     /locus_tag="VFR82_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU4501525.1"
                     /translation="MPLYEYRCGRCEKHFDVTQSVHARVEDTVCPFCQAQEATRLLSS
                     FVSTVKGDHKPGFKEMKAYEMHNKRASSFSKLPPLFGARSAPPPIQDVFSGSESQPET
                     PPEKTGS"
     gene            1531..1707
                     /locus_tag="VFR82_07735"
     CDS             1531..1707
                     /locus_tag="VFR82_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501526.1"
                     /translation="MAVLIRRYKGHNGMMQEERIDDDDRIERYFRLFEKEDVKKLETG
                     VKVFIEKDEWQLLP"
     gene            2256..3116
                     /locus_tag="VFR82_07740"
     CDS             2256..3116
                     /locus_tag="VFR82_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4501527.1"
                     /translation="MPRLALLLTTFIWGATFPATKAALEQMPPLTFLCLRFLLGTLLV
                     LAWFALMRHRLRREGGVLSASAIATIFLFFGYVLQTVGLYYTTASNSAFLTALYVIIV
                     PLMLRRFDRRVIAGTTIATVGLWLLVKPSAVVNVGDVMTVGCAVAFAGHIVCLERYTR
                     LFDAPSLLLWQVSAMTLLFLPMMAWEQPPAHAFTPSTVLMIGLAVTGIFATGAFAVQM
                     WAQRLIPAQQVALIFASEPAYAAWLSWYFLGETLDLQGWIGSGLILIAVLIGAFASSP
                     QEAAAVVEPA"
     gene            3130..3600
                     /locus_tag="VFR82_07745"
     CDS             3130..3600
                     /locus_tag="VFR82_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501528.1"
                     /translation="MAQKFGNGRWVQEGFLDNRIQGTVVGRIVFAAIGPVDLYLRGDF
                     KLDIAGQVIQFRNTHFEDDELAGHVIGDIENPQIGTVNLISFDPHPNMVPHPYVEWFS
                     TRSDHYRFELDPEDAWLVPTSDWEIIDQQSRQIRAALAAQTIERPKREESDWMM"
     gene            complement(3613..4608)
                     /locus_tag="VFR82_07750"
     CDS             complement(3613..4608)
                     /locus_tag="VFR82_07750"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEU4501529.1"
                     /translation="MSDVRTLQPTKAQDLWFTILRWFDSWAGLEHMKTDGVPKMDWLR
                     CIPIIAVHAMCLGVIWVGTSWTAVAVAVVFYFVRMFAITGWYHRYFSHRTFKTSRAVQ
                     FAFAVLGGTCAQRGPLWWAGHHRHHHIASDSADDVHSPRQGGFLWSHIGWFTSQTHFA
                     PRLKGIADFAKYPELRFLDRFDILVPTIAGFAMFGFGKVLEIYAPHLGTNGPQMLIWG
                     FFISTVALIHGTCTINSLSHVYGSQRYETGDDSRNNFLLALITMGEGWHNNHHFYPAS
                     TRQGFYWWEIDMTYYCLKLMEWLGLVWDVRGVPVYVREQKRKEDTIGVPLTAVES"
     gene            5004..6029
                     /gene="hemE"
                     /locus_tag="VFR82_07755"
     CDS             5004..6029
                     /gene="hemE"
                     /locus_tag="VFR82_07755"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEU4501530.1"
                     /translation="MNDRFLKACRREPVDCTPVWFMRQAGRYMAEYRSLRVKHSMLSL
                     CKTPELAAQVTLQPIDRFPLDAAIIFADILLPLEAMGLNLEFAEGEGPVIHNPVRTRA
                     DVDRLKAIDGTELEYVAEAVRQARKALNGRVPLIGFAGAPFTLASYAIEGGGSRNYVL
                     TKSLMYREPDVWHQLMDKLARVVTGYLRRQIRAGAQAIQLFDSWVGCLSAGDYAEYVK
                     PHVQLIFAGLEREGVPMIHFGTGTTAILRQMREAGGDVIGVDWRIHIDEAWAVIGHDR
                     AVQGNLDPVALYAPLHEIERRVEDILRRAGGRNGHVFNLGHGILPTTPVEHVEAAINI
                     IHKLSMR"
     gene            6107..7048
                     /gene="hemH"
                     /locus_tag="VFR82_07760"
     CDS             6107..7048
                     /gene="hemH"
                     /locus_tag="VFR82_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU4501531.1"
                     /translation="MPKPLAVLLMAMGGPDCLENVEPFLLDVRGGRPTSPELVAEIRE
                     RYRATGGKSPAVGITTKVATKLERRLNESGRAQYRVYVGLRHWHPFIKETYARLMKDE
                     PDHVIGLPMAPQQSSLSTGAYRKKVEEAQAGLSDHRSVSFVGSWNRHPRLIAAIAENV
                     QEGLRKFSSPVRADVTVLFTAHSLPERIVTMNDPYPDEVKGTVAAVTALLGKQATAFA
                     YQSQGRSGESWLGPTVEATVEELARAGRRHVLVAPIGFLCDHVETLYDIDIELKQSAA
                     RSDVQLERIAMLNDGPALIETLADVVAAHESSLAIPS"
     gene            7045..8493
                     /gene="hemG"
                     /locus_tag="VFR82_07765"
     CDS             7045..8493
                     /gene="hemG"
                     /locus_tag="VFR82_07765"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEU4501532.1"
                     /translation="MKNPSTVAIVGGGISGLATAHALMERAEAKGVALRCTVLESGAA
                     WGGKIVTHTIGDLVTEAGPDSFLSSKPAGLQLVEKLGLNDQLINTNETGKKAFVYLGG
                     RLRELPEGLIAIAPGQIRPFLKSGLLSFGGFARMGMDLVLPAKRSSVDESLASFVRRR
                     FGQQAFDRMMEPLMAGIYAGDAEELSVRATFPRFVELEQEHGSVLRGMMSVRARQNGA
                     GRTEAKRTMFVTLKRGLQELVTALVRRLTDQGVLLRSGMTVESLRVRSNQVGRWTYDM
                     VLQDRSSLSADSLVLATPAYVAADLLRPLTPIAGGLLETISYASTATIALAYPASVIG
                     GAVQGFGFVVPRIEKRDLIAATWTSLKWPHRAPPNQLMVRCYVGGAGRESILALDDPA
                     LVGRVKTELREICGIVAEPTYTEVNRWMHAMPQYRLGHLERLAQAEVALSRYGGVFLT
                     GSAYRGVGIPDCIRDGAVAADKVMRYLSGERL"
     gene            8506..8919
                     /locus_tag="VFR82_07770"
     CDS             8506..8919
                     /locus_tag="VFR82_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501533.1"
                     /translation="MPATNALKILGVAGPFREPREPAFSYDYSIQRPQWPMPHAVRVK
                     VAIAEELDVLRGKILGPVSGSPGQQLLVSQLLTRRIADEKLRIADQDGLLSGRADVVV
                     APFAGPLAHLFPRLESWLDQHGEAVRIEIKERVKI"
     gene            complement(8981..9598)
                     /locus_tag="VFR82_07775"
     CDS             complement(8981..9598)
                     /locus_tag="VFR82_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501534.1"
                     /translation="MPQEEGPYQEGKRAGLHPLVVVFGVLLGLWLFVALIVPSSRNKQ
                     ATGTEGPAGPGVEDPEAAPVIFKVQATVPEMNAVSLVVPPQATESQVVGLLKRLKKER
                     LAGTLTDLIPATTPGHKLGDHAVAEVYVLSDPIYAEADVIRTLSRGAHAPGGLYPDRV
                     PFEVAMEEIRGHYRIDLNDTGAPDAGSIGFADESGVHSKHFRKIF"
     gene            complement(9598..10446)
                     /gene="mazG"
                     /locus_tag="VFR82_07780"
     CDS             complement(9598..10446)
                     /gene="mazG"
                     /locus_tag="VFR82_07780"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEU4501535.1"
                     /translation="MSQRFQKLMDLMAALRAPNGCPWDRKQTHESLKPYLLEETYEAL
                     ETIDRGDHKKLAEELGDVLLQVLFHSQIAAESGTFTIDDVLNRLADKLVRRHPHVFPN
                     GADDPTPTNAEQVLAKWEEIKRAERQAEGRPASAIDGVPQTLPALLRAYQVQARASRV
                     GFDWSHDQAGFDQVLTKIEEEVRELRAALRPPPEEGDTGARQREITAEFGDVLFSLVN
                     LSRFVKVNPEDALRQAVNRFAERFHYIERQADQAGRGVADLSFDEMNRWWEEAKQQES
                     QRDARR"
     gene            10564..10950
                     /locus_tag="VFR82_07785"
     CDS             10564..10950
                     /locus_tag="VFR82_07785"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEU4501536.1"
                     /translation="MAGEKEEGFVIRDRRGSGAGSEQPAPPSAAAQEARSSPTPGTET
                     GSGVPVTFASFVISLGSSSLMLMGEQLDPQQPAMPVNLPQAKEIIDLLSVLDAKTKGN
                     LSTEEQTVLRDMLYALRMKYVSLTSK"
BASE COUNT         2263 a         3152 c         3352 g         2404 t
ORIGIN      
        1 taatcacttc cgctcgaacg gaatatagga ttcggcgata atttgtcgcc cagcaggcga
       61 taaggcgaat tgcccaaagg cttcgaccaa cggattcgga tccttctttg acagcagcaa
      121 gacgggacga cgaagcggat aacgtccgtc tttgacagtg ggtatttccg gttccactcg
      181 gtcaacgggc agtaaccgga cggcgacgcc gctggtgacc acagataagc cctgggtcag
      241 ggaaatatat gccacggcag acatgggagg aagtgtgccg acaacggtct tgattacttt
      301 gtcgtctttc gcgatgattt tggcgttgtc cggaattttt ccatcaatgc ccagttggcg
      361 ctcgaacgcg tcgcggatgt tctgattttt cgggcgatcg atcaacagaa tcttcgtttc
      421 cgggccgccc agctccgacc attccttgac tttgcctgag aacatgtcgg ccacctgctg
      481 tttcgtgact tctttcgtga agttcgagag gtggacgaga atgccgatgc cgtcccaggc
      541 aatttgtgtc gcagcaaggc ctgagtcttc ggatccggtc accgcgatat gggcatcacc
      601 cgccttgacc atctcaaccg gcttggaatg atcatcccag atcacatcga catacgcccg
      661 ggggttcgct ttctcaaagg cgcgggagag tgattcgatg gtggtgagct ccggtccatt
      721 tccaacgatc acgagattgc ccgacacctc cgcctgaaca aaggcaggga atgcggtcgc
      781 gagtacgaac cctgcggcca tgagcggcga cacataagca gaaagagctt ggaatttgcg
      841 gttgggaatc cggaagggga gcgaaagagt cgacgagtcc atgcggcaaa atcagcttcc
      901 ggtcttttcc ggaggagtct cgggctgcga ttcggaacca gagaagacgt cctggattgg
      961 tggaggcgcg cttcgcgccc cgaacaacgg tggcagtttc gaaaaactgg atgcccgctt
     1021 gttgtgcatc tcgtacgcct tcatttcctt gaagccgggc ttgtgatcgc ccttgacggt
     1081 cgagacgaaa gaggaaagca gacgtgtcgc ctcttgggcc tggcagaagg gacagaccgt
     1141 atcttccact cgagcatgga cggattgggt gacatcgaag tgtttttcac agcgtccgca
     1201 tcggtactcg tagagtggca tatcgtttcc ccgccagatt tgacaatcac cgggcggatg
     1261 cgcccttgac catccgttaa agtttgcctt attgtaacac acctgcctgg ttccaaaact
     1321 agcagggatt taacagggtt ggaacgttaa ccggcggaat atcatctaga gtggcctcgt
     1381 gcagggagtt tagaaagctc gcacagatac gtgcgccggt tcggaggagg cacgtggtac
     1441 gtacgtgggg cctgttgaca cggtaaaaaa ggcgggcgga ctgtttcaac tttcctgaag
     1501 ggaccgagtc atgatgaggg aggatgtgcg atggcagtgc tgattcgacg atacaaaggt
     1561 cacaacggca tgatgcagga agagcggatc gatgacgatg atcgaatcga gcgctatttt
     1621 cggctcttcg agaaagagga cgtcaaaaaa ctggaaaccg gtgtcaaagt gtttatcgaa
     1681 aaggacgaat ggcagctgct tccctaatgg acgggcggtc ccacaccatc cgctattgac
     1741 aacacttgca tgatttattg gatacacgtc gcgcactcca tcgatcgggt gacgttgcac
     1801 aaagatcgat gctctgaagt gccatcttcg cagacctcaa ctgactattg ggggggaggc
     1861 tggtacgatt atccggacaa ggagcgcgcg ctcgcggcga tggaacaagc tggaccgacg
     1921 gacctacgcc gctgcccgct ctgcaaacct tagcgggatg ttgaaaacgg catcattctc
     1981 acccgccgga acgcgtcttg acccggactt tgtcctcgcg tcgcttagag cctcaacata
     2041 ccgatttcac tggaggccaa gtgcttctcc gctcgacttt gatcgaagcg agcggctcaa
     2101 gggaagcttg gtatgtacct tatcggctct gtgcttgctg cggcctcggt tgcggaggga
     2161 ggcgtcccgg cgcgtcgggg tcgggcgggt gagaccagcg gcctgtttga acatccctga
     2221 agagccctca tacaactcac acttctccgg attccatgcc tcgactcgca ctgctgctca
     2281 ccacctttat ttggggggca acgtttcctg cgaccaaggc ggctctggag cagatgccgc
     2341 cattgacctt tctgtgcctg cgtttcctgc ttggcacgct gctcgtcttg gcctggtttg
     2401 cgctgatgcg tcatcgcctt cgccgggaag gcggtgtgtt atctgcaagc gcgatcgcca
     2461 caatcttcct gtttttcggg tacgtgcttc agacggtcgg cttgtattac accaccgcct
     2521 cgaacagcgc atttttgact gcgttgtatg tcattattgt gcctctcatg ttgcgccgct
     2581 tcgatcgccg tgtgatcgcg ggcacgacga ttgccacggt gggattgtgg ctgctggtga
     2641 agccgagtgc tgttgtgaac gtgggcgacg tcatgacagt cggatgtgcc gtcgcctttg
     2701 ccgggcatat cgtctgtctg gaacggtata cccgtctctt tgatgcgccg tcgttactct
     2761 tgtggcaggt cagcgcgatg acgctgctgt ttttgccgat gatggcgtgg gagcagcccc
     2821 cggcccatgc ctttacgccc tcaacggtgc tcatgatcgg tcttgcagtc acgggcattt
     2881 ttgccacagg ggcgtttgcc gtgcaaatgt gggcgcagcg actgattccg gcccagcaag
     2941 tcgcgttgat ttttgcgtcc gagcctgcgt atgcggcctg gctctcatgg tattttctcg
     3001 gggaaacact cgatcttcag ggatggattg gaagcggatt gattttgatc gcggtgctga
     3061 tcggcgcctt tgcatcaagc ccgcaagagg ccgccgcagt cgttgaaccg gcgtagcggg
     3121 agagtgtcga tggctcagaa gttcggcaat gggcggtggg tgcaggaagg gtttctggat
     3181 aatcggattc aagggaccgt cgtgggccgt atcgtgttcg ctgccattgg tccggtcgat
     3241 ctgtatctgc gaggggattt caagctcgat attgccggcc aggtcatcca gtttcggaac
     3301 acccatttcg aggatgatga gttagcgggt cacgtgatcg gcgatatcga gaatccacag
     3361 atcggaaccg tcaacctcat ttcattcgat ccccacccga atatggtccc gcatccttac
     3421 gtggaatggt tttccacgcg atccgatcat tatcgatttg aactggatcc ggaagacgcc
     3481 tggcttgtgc cgaccagtga ctgggaaatc attgatcagc aaagccggca gattcgagcg
     3541 gcattggccg cacagactat cgagcggcca aagcgggaag aatcggactg gatgatgtaa
     3601 gcgtcggacc gtctaactct ctactgcggt gagcggaacc ccgatcgtgt cctcttttcg
     3661 tttctgctct cgaacgtaga caggcacacc tcgaacatcc cacactagcc ctaaccactc
     3721 catgagcttc aggcagtagt aggtcatgtc gatttcccac cagtagaatc cctgccgcgt
     3781 cgaagcagga tagaaatggt gattgttgtg ccacccttcg cccatcgtga tcagtgccag
     3841 gagaaaattg ttgcggctgt cgtcgcctgt ctcatagcgc tgcgacccgt acacatgaga
     3901 gagggagtta atcgtgcagg tgccgtgtat gagggcaacg gtcgaaatga agaaccccca
     3961 aatgagcatt tgcggcccat tcgttccgag atggggggca tatatctcca acacttttcc
     4021 gaaaccgaac atcgcaaaac cggcgatcgt gggcacgagg atgtcaaatc ggtccagaaa
     4081 acgcagttct ggatacttgg caaagtcggc aatgcctttt aaccgcgggg caaagtgcgt
     4141 ctgcgaggta aaccatccga tatgagacca cagaaagcct ccctgacgag gcgagtgcac
     4201 atcatcggca gaatctgagg cgatatgatg atggcgatga tgaccggccc accagagggg
     4261 cccgcgctgc gcgcaggttc cgccgagcac ggcgaatgca aactgcacgg cacgggacgt
     4321 cttgaacgtg cggtgtgaga aataccggtg ataccaaccc gtgatcgcaa acattctcac
     4381 gaaataaaac acgaccgcga ccgccactgc cgtccagctg gttcctaccc agatgacgcc
     4441 caggcacatg gcatgtacgg cgataatggg aatgcaccgc agccagtcca ttttggggac
     4501 gccgtccgtt ttcatgtgct cgaggccggc ccaggaatcg aaccatcgca aaatcgtaaa
     4561 ccaaaggtct tgcgccttgg tcggttgaag agttcgcaca tcggacattc ggattacctc
     4621 caattaatcc cggcgcattc cgccggacac acacagcgtt ctgaatctca gtaaggcctc
     4681 ggcacttgat ggtgtttcac gaggcgggct gcacaatcca aggcgctata gacgctccat
     4741 caccagccct ggacgacggg attgcattat acatcaaatt tctccaaata gtaggcttgg
     4801 tcggattctt taattgtacc ggatagttcc gctctgtcta ctcaagcgta aaatcacgaa
     4861 attggtgggc gggaagtggc gaaaattgac cagctattgc tcttcagtct gaggtctgtg
     4921 cgagggaaca ccgacctttt gaacgcgaac tcgatcaggc atcaatagag actttaatcc
     4981 catcctggta gaatgccgcg cgtatgaatg accgctttct gaaagcctgc aggcgggagc
     5041 cggtcgattg cacgcccgtt tggttcatgc ggcaggccgg tcggtacatg gccgagtacc
     5101 gctcgcttcg cgtgaagcac tccatgctct ctctttgcaa aaccccggag ttggcggcac
     5161 aagttacgct tcaaccgatt gaccgctttc ccctggacgc cgcaattatc tttgccgaca
     5221 ttctcctacc cttagaggcg atgggattaa acctggaatt tgccgagggc gagggaccgg
     5281 tcatccataa tccggttcgg acgcgcgccg atgttgaccg cctgaaagcg attgacggaa
     5341 cagaattaga gtatgtggct gaggccgtga gacaggcgcg gaaggcattg aacggccggg
     5401 tgccgctcat cggatttgcc ggggcgccgt tcacgcttgc cagctatgcg atcgaaggcg
     5461 gcgggtcccg caactacgtc ctgacgaaaa gcctaatgta tcgtgaaccg gacgtctggc
     5521 atcagctgat ggacaagctg gccagggtcg tcaccggcta tctgaggcgg cagatcaggg
     5581 cgggagctca agccattcaa ctgttcgata gctgggtcgg ttgtctctcg gccggtgatt
     5641 atgccgagta tgtcaaaccg cacgtgcagt tgatttttgc cggattggag cgcgaaggcg
     5701 tacccatgat ccatttcggc accggcacga cggcgatttt gcggcagatg cgtgaagcgg
     5761 gcggagatgt catcggcgtg gattggcgca ttcacattga tgaggcctgg gccgtgatcg
     5821 gacacgatcg cgccgtccag gggaacctcg atccggtcgc gctctacgca cctctccatg
     5881 aaatagaacg tcgcgtcgag gacatcttgc ggcgcgcggg gggccgcaac ggacatgtgt
     5941 tcaatctcgg gcacggtatc ctgccgacca cacccgtcga acatgttgag gccgccatca
     6001 acatcatcca caaactgagc atgagatagc agcgcggccg ctagctggcc cgtacatgag
     6061 tcccgccgcc tgctactgag ctgacacgag aggattgatc gcccaaatgc cgaagccact
     6121 cgcagtgctg ttgatggcta tgggaggccc cgactgtctg gagaacgtcg agccattcct
     6181 cctcgacgtg cgcggcggtc gtcccacttc gccggagctc gtggctgaga tacgggagcg
     6241 ctaccgggcg accggtggaa agtctcccgc agtcggcatc acgacgaagg tggcgacgaa
     6301 gctcgaacgg cggttgaacg agtccggccg tgcccaatat cgggtctacg ttggcctgcg
     6361 gcattggcac ccgttcatca aagagaccta cgcccggttg atgaaggacg aacccgacca
     6421 tgtcatcgga ttgcctatgg ccccgcagca gtcgtcgtta agcacgggtg cctaccgcaa
     6481 gaaggtggaa gaggcccagg ccggcttgag cgatcatcgc tcggtgagtt ttgtcggaag
     6541 ctggaaccgg cacccccggt tgatcgccgc catcgccgag aacgtccaag aggggctccg
     6601 caagttttct tccccggtac gggcggacgt gacagtcctc ttcactgccc atagcctgcc
     6661 ggaacggata gtgaccatga acgatccgta tccggatgag gtgaagggca cggtcgcagc
     6721 ggtgaccgct ctattgggca aacaggccac tgcgtttgcc taccaaagcc agggacggtc
     6781 aggcgagtcc tggcttgggc ccacggtcga ggcgactgtt gaagagttgg ctcgggcggg
     6841 acgacggcat gtgctcgtcg ccccgatcgg tttcctgtgc gatcatgtcg agacgctata
     6901 cgatatcgat atagaattga agcaatcggc ggcgcgaagc gacgttcagc ttgagcgcat
     6961 cgccatgttg aacgatggtc cagccttgat cgaaaccctc gcggatgtcg tcgccgccca
     7021 tgaaagttcg ttagccattc cctcgtgaag aatccctcga cagtcgcgat tgtcggtgga
     7081 ggcatttcag gcctggcgac ggcccatgcg ctcatggagc gggccgaggc gaagggagtg
     7141 gcgcttcgtt gtacagtgct ggaaagcggt gcggcctggg gcgggaagat cgtcacccac
     7201 accatcggcg atctcgtcac cgaagccggc cccgattctt ttctgtccag caaacccgcc
     7261 ggtttgcagc tcgtcgaaaa gctcggcttg aacgatcagc tcatcaatac gaacgaaacc
     7321 ggcaagaagg cgtttgtcta tctcggtggc cgactccgag aattgcccga aggcctcatc
     7381 gccatcgcgc cgggacaaat ccggccgttc ctgaaaagcg gcttgttgtc cttcggaggc
     7441 ttcgcccgaa tgggaatgga cttggtcttg cccgccaagc ggtcctcggt tgatgaatcg
     7501 ctcgcctcgt ttgtccgccg gcggttcgga cagcaggcgt tcgaccgcat gatggaaccg
     7561 ttgatggcag gcatctacgc cggcgacgcc gaagaattga gtgtccgcgc gacgtttccg
     7621 cgctttgtcg agctggagca ggaacatgga agtgtcctgc ggggcatgat gagcgtgcgt
     7681 gcgaggcaga atggcgccgg gcggactgag gctaagcgga cgatgttcgt cactctgaag
     7741 cgtggtctcc aagagttggt gacggcgttg gtccgccggt tgaccgatca gggtgtcctg
     7801 ctgagaagcg gcatgaccgt ggagtccttg cgagtacgct cgaatcaagt cgggcggtgg
     7861 acctacgaca tggtgctcca agataggtcg tctctttcgg cggatagtct cgtgctggcg
     7921 accccggcct acgtagctgc ggatctgtta cggccgctga cgccgatcgc ggggggcctc
     7981 cttgagacga tctcctatgc gtcgaccgcg accatcgcac ttgcgtatcc ggcctccgtc
     8041 attggcggag ccgtacaagg gtttgggttt gttgttccgc gcattgagaa gcgggacctc
     8101 atcgccgcca cctggacgtc cctcaagtgg ccgcatcgtg cgccacccaa ccagctcatg
     8161 gtccgttgct acgtgggcgg ggcgggacgg gaatccattc tcgcgctgga tgatccggcg
     8221 ctcgtcgggc gcgttaaaac ggaactgaga gagatctgcg gtatcgtggc cgaaccgact
     8281 tacacggaag tgaatcgttg gatgcacgcg atgcctcaat accggctcgg acacctggaa
     8341 cgtctcgcgc aagctgaggt tgcattgagc cggtacggcg gggtatttct cacgggctcg
     8401 gcttatcgcg gcgtcgggat tcctgattgt atccgcgatg gcgcggtcgc ggcagacaag
     8461 gtcatgcgat acctctcggg agaacgtcta taaaggatag gtacgatgcc ggcgacgaat
     8521 gcgttgaaga ttctcggagt ggctggtcct tttcgtgaac cgcgcgaacc ggcattttcc
     8581 tacgactatt ccatccagcg gccccaatgg ccgatgcccc atgccgtgcg cgtgaaggtg
     8641 gccattgcgg aagagctgga cgtgttgaga ggcaaaatac tcggccccgt cagcggctct
     8701 cctgggcaac agctgctcgt gtcgcagctc ctcacacgaa ggattgccga cgaaaagtta
     8761 cggatcgctg atcaggacgg cctgctgtcg gggcgtgccg atgtcgtcgt cgcccccttt
     8821 gctggtccgc tcgcgcattt atttccccga cttgaatcct ggctcgacca gcacggcgag
     8881 gccgtgcgta tcgaaatcaa ggagcgggtc aaaatttaga acgctgctgg aagagtctgg
     8941 atgggtagtg cccagctctc gaacactcca gaggaaccgt ctagaagatt ttcctaaaat
     9001 gcttggaatg aacccccgac tcgtctgcaa acccgatcga gccggcatcc ggtgcgccgg
     9061 tatcgttgag atcgatgcgg tagtgcccgc ggatttcttc catggccact tcgaagggaa
     9121 cgcgatcggg ataaagaccc ccgggcgcgt gggcgccccg agaaagtgtc cgaatgacgt
     9181 ctgcctcggc gtagatcgga tccgaaagca catagacttc cgccacggca tggtcaccca
     9241 gcttgtggcc gggagtcgtt gcgggaatca gatccgtgag tgtcccggcc aggcgttctt
     9301 tcttcagccg tttcagtagc cccactacct gactttctgt tgcctgcggc ggcacgacca
     9361 ggctcacggc attcatctcg ggaacagtcg cctggacctt gaagatcact ggagcggcct
     9421 caggatcctc aacgccgggg ccggcggggc cttccgtccc ggtggcctgc ttgttccgcg
     9481 aactcgggac gataagggcg acaaacagcc agagccccag caggacgccg aagacgacga
     9541 ccaggggatg caggcccgca cgcttgcctt cctgataagg accttcctct tgtggcatta
     9601 ccgcctcgcg tcgcgttgcg actcctgctg tttcgcttct tcccaccacc ggttcatctc
     9661 gtcgaatgac aggtcagcga caccgcggcc tgcttggtcc gcttgtcgtt cgatatagtg
     9721 aaatcgttcc gcaaaacgat tgaccgcctg gcgcaacgca tcttccggat tcactttgac
     9781 gaagcgggaa agattgacga gcgaaaacag gacatctccg aactctgcag tgatctcccg
     9841 ttgtctggcg cccgtgtctc cttcctctgg cggtggcctc aaagccgcgc ggagttcgcg
     9901 aacctcctcc tcgatcttgg tcaacacctg atcgaacccc gcttggtcgt gactccaatc
     9961 gaaccccacc cgtgatgccc gtgcttgtac ttgatatgcc cgcaagagcg ccggaagggt
    10021 ctgcggcact ccgtcgatcg cggatgccgg gcggccctct gcctgacgct ccgcgcgttt
    10081 gatttcctcc catttggcga ggacctgttc ggcattcgtt ggggtgggat cgtcggcccc
    10141 gttagggaaa acatgggggt gccgcctcac aagcttgtcg gccagtcggt tcagcacatc
    10201 gtcgatggta aaagtgccgg actccgccgc aatttgacta tgaaaaagga cctgaagcag
    10261 gacgtcgccc agttcttcag caagcttttt gtgatcgcct cgatcgatgg tttcaagggc
    10321 ttcgtaggtt tcttcgagca ggtaaggctt caacgactca tgcgtctgtt tccggtccca
    10381 tgggcagccg tttggcgcac gcaacgccgc catcagatcc attaatttct gaaatcgctg
    10441 ggacaacagc ccggctcctt gtcgttgccg aaggcgctat tataccccgt tcctcatggg
    10501 cttcaacgag ttgcgtgcct gcagggccta tggtaggctc gaaaatcctt catggaggca
    10561 gccgtggcag gcgaaaaaga ggaagggttc gtcattcggg atcgtcgagg cagcggcgct
    10621 gggtccgagc aaccggctcc gccctccgcg gcagcgcaag aggcccgttc ttcgccgaca
    10681 ccaggcaccg aaaccggcag cggagtgccc gtgaccttcg cttccttcgt gatctcgctc
    10741 ggaagctcgt ccctcatgct catgggagag caattggatc cgcagcagcc cgccatgccg
    10801 gtcaatttgc ctcaggcgaa agagatcatt gatctgctct cggtcttgga tgccaagacg
    10861 aagggtaatc tctcgaccga agaacaaacg gtcttgcgcg atatgctcta cgccctacgc
    10921 atgaaatatg tcagtctgac ttcgaagtaa acctgcttgt cctgcactcc tgctcctcac
    10981 cggagcctag cagccttttg agcctggcct ctatttccgc tatagtgggt gctcttaccc
    11041 gtcatacgat cgagccacgg agggggctcc cggggtgact gaacagcgga aaagcattgt
    11101 gactcccgtc tggaccggtc ccgagcgccg ggcggcgccg gacgggccgc gcggcggatc
    11161 aacttctgag g
//
LOCUS       DASTQY010000237         6690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1027884, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000237 DASTQY010000000
VERSION     DASTQY010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027884"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFR82_07790"
     CDS             <1..604
                     /locus_tag="VFR82_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501537.1"
                     /translation="YFHRTTRAIDIHLRDIFGDTMKLLFPHDPRKKMDAYLTLTDSSL
                     LETVRGWKTSRHNGERRLGEEWARILGRDVKWKMAYSTVLKEKGQERGMDFPSHSHFE
                     KQIRKELPRHLQQVEFRVDMAPLDPRPDPKDQRGTPLYVYDPGTRTVSTEPLEEFLDL
                     LPTRLIQFRIYSLDHRHDAVLSRAAGTVLNKTPSSIESQY"
     gene            complement(690..1691)
                     /locus_tag="VFR82_07795"
     CDS             complement(690..1691)
                     /locus_tag="VFR82_07795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU4501538.1"
                     /translation="MILPALKQVSQRGSRLDRMLITTIVLLTSVFPVELFPNAPPLPK
                     GIDGQYSGRQGQVVLVKVAVREEPTDVKGIFLDRTIPFFREMRTGEPSGYLGLVGIDM
                     QDAPGTYELAVTVKYGEKVQRLSYHVLVTKEKFAVEHLKLPKDKVDLDEKTLTRWKSE
                     QEQVRRALADNSALRLWQSGFVEPVNGKRTGIFGSVRIMNGQPRNPHNGEDIGAPLGA
                     DVAATNDGVVRLVVDHVFSGRGVFIDHGLGFYSMYFHLSEVLVKEGDLIQAGQIIGKV
                     GATGRATGPHLHWGVKLNGARVNPYALLQLPFPSRSLPGASPPSSSEISPEALPVKE"
     gene            complement(1775..3169)
                     /gene="pafA"
                     /locus_tag="VFR82_07800"
     CDS             complement(1775..3169)
                     /gene="pafA"
                     /locus_tag="VFR82_07800"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HEU4501539.1"
                     /translation="MPSSGPMKQRIFGLENEYGLIFSPNGRIYLPMEKVLGYIFEGLI
                     PNSWPSNAFLVNGARFYQDTGCHPEYSTPECDNILDLVVHDKAGERLLEACLPAAEER
                     LREEGLSGEIYIFKNNTDSLGNTYGCHENFLMRRDVDFWKVTEQLIPFFVTRQIYSGA
                     GKVLKVSGKPQFFISQRAQHIHEKTSSSTTSSRSIINTRDEPHADAERYRRLHIIVGD
                     SNMSELVTYLKVGTATLVLSMIEEGFSVHGMELEDPVKAIREISRDPTVKRKVKLDDG
                     RQMTAVEIQRVYLERAHDYLTQQDHDPILDDVWKRWATILDKLEEDPMQLVREIDWIT
                     KRHLIQSYVDKKGCSWDDPRVFLLDLQFHDVKRTRGLYYLMESRGLVERVVEEDAVQR
                     AMSTPPQTTRAKVRGDFIRCARAKNRSYTVDWTYLKLNGYWEETILCMDPFSAVNRRV
                     DELVSQVSGLRFYR"
     gene            complement(3244..3927)
                     /gene="prcA"
                     /locus_tag="VFR82_07805"
     CDS             complement(3244..3927)
                     /gene="prcA"
                     /locus_tag="VFR82_07805"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HEU4501540.1"
                     /translation="MPMPYYVSPEQMMQDKAEYAKKGIAKGRSIIAMEYVDGILLAAE
                     NPSASLHKISEIYDSIAFAGAGKYSEFENLRKAGIRHADLKGFMYSREDVTARSLANG
                     YSQSLGTIFSQEMKPLEVEILVVQIGHNGHTNEMYRISFDGSIIDEKQFAVIGGKSEV
                     VQAALKEKVPAQPPDLKTALNLSILALEQTGNQKLSLETLEVAVLDRTRENRKFRRYT
                     PAETKQFLA"
     gene            complement(3930..4760)
                     /gene="prcB"
                     /locus_tag="VFR82_07810"
     CDS             complement(3930..4760)
                     /gene="prcB"
                     /locus_tag="VFR82_07810"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEU4501541.1"
                     /translation="MKLPFLPNHDDASFFDFLTRQHPELTPGGRRPTEPPLPVHHELP
                     RSVSPVTVPQATTVLAVKSQEGVVIAGDRRATEGFQIAERRIEKVFKIDDYSAMAIAG
                     AAGPCIEMAKLFQTELEHYEKLEGMQLSCEGKANKLGQMVKANLPMVFQGLVVMPLYV
                     GYDLKRGEGRIFKYDLAGGRYEESDYHAIGSGGKDARNTMREHFKKAMTEQEALKLAL
                     LALYNAADDDVGTGGPDLIRGIYPTAKIVNAQGITDVSESDIRSVYEAMLATRIAKER
                     "
     gene            complement(4760..6259)
                     /gene="dop"
                     /locus_tag="VFR82_07815"
     CDS             complement(4760..6259)
                     /gene="dop"
                     /locus_tag="VFR82_07815"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HEU4501542.1"
                     /translation="MRDSTPPTTPRVLGTETEFGIASRDASAVDPISNSLAVIGHYPG
                     ISVPHAVWDYENENPLLDARGFEVEGERERPNPDYNRQLNKVLANGGRLYVDGAHPEY
                     STPECTNPREVVAYERISERILGQCLDNMARLRGRDQFVLYKNNSDGKGNSYGYHENY
                     MLSRSVPFERIVKTLAPFFVTRLIYAGAGKVGAENQTSFTEFQISQRADFFECLVDLN
                     TMVKRPIVNSRDEPHADYGKYRRLHVIVGDANMAELSTYLKVGTLGIVLDLLEAGVDV
                     PEIELDDPVMGIKNVSRDLTMKGTLKLTDGRSTTAVEIQRAYLKSAMNFYACHELDQV
                     TKDVLVRWEDVLDKLERDPRSLAGELDWAAKRHMIESYMDRKGCGWDDPRVRLMDLQY
                     HDIRPDRGLYYTLERSHLIERVVQDVEVIRAEFTPPSGTRAYFRGRCIGKFSKSIYGA
                     SWTSVLFDVGNTTIKKVPLMDPLRGTESLTAELLDQSDTAEILLSKLKA"
     gene            complement(6266..>6690)
                     /locus_tag="VFR82_07820"
     CDS             complement(6266..>6690)
                     /locus_tag="VFR82_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HEU4501543.1"
                     /translation="HGGDTKALVEHLTDLTVGAMYATSEENKFIEVTYANGEKEILYF
                     KDFASGALIEGIVSRAKKYAVKRALAQEGRGLRSDDLIRAIREEFKEHEDLPNTTNPD
                     DWAKVAGKKGEKIVHIRTISGGPAESRQIETITTGHYL"
BASE COUNT         1389 a         1978 c         1809 g         1514 t
ORIGIN      
        1 gtactttcac cggacgacga gagccatcga catccatctt cgcgacattt tcggggacac
       61 tatgaagctg ctgtttcccc acgatccgcg caagaagatg gatgcgtatc tgacgctgac
      121 tgattcctcc ttgctcgaaa cggtacgcgg gtggaaaaca tcccgtcaca acggcgaacg
      181 gcgtctcggc gaggaatggg cacgcatttt aggacgtgac gtgaagtgga aaatggcgta
      241 cagcacggtg ttgaaggaaa aggggcaaga acggggaatg gacttcccga gccattcgca
      301 cttcgaaaag cagattcgaa aggaactgcc caggcacctc cagcaggtcg agttccgcgt
      361 agacatggcg ccgctcgatc cacgcccgga ccccaaggac cagcggggca ctcctctgta
      421 cgtatacgac cccggaaccc gtaccgtttc caccgagcca ctcgaagaat tcctcgattt
      481 gttgccgacc aggctgatcc aattccgcat ctattcgctg gatcaccggc atgacgcagt
      541 gctttcccgc gccgctggga cagtgctcaa caaaacacca tcgagcatcg aatcacagta
      601 ttaaacgagc gtggcaattc gtcagcggga tcgtctagaa caggcccccc tgaaggcttg
      661 tgtttccaca gagcgtcgtc accgacacgc tactccttga ctggaagcgc ttcgggggag
      721 atttcggagg aagacggcgg actggcgccg ggcagagatc ggctcggaaa cggaagctgg
      781 agcagtgcgt aggggttcac ccgggcaccg ttgagtttca cgccccagtg caaatgcggg
      841 cctgtggcgc ggccggtggc ccccaccttg ccgatgatct gccccgcctg tatgagatct
      901 ccctctttta cgagaacttc ggagagatgg aaatacatac tgtaaaaccc caacccatgg
      961 tcgatgaaga caccccggcc tgaaaatacg tgatccacga ccaggcgcac cacgccatcg
     1021 ttggtcgccg ccacgtcagc tcccagcggg gcgccgatat cctctccatt gtgggggttt
     1081 cgaggctggc cgttcatgat ccggacactg ccaaaaatac ccgtgcgctt cccattcacc
     1141 ggctccacga aaccgctttg ccagaggcgc aatgccgaat tgtccgcaag agcccggcga
     1201 acctgctcct gctcagactt ccagcgtgtc agggtcttct cgtccagatc caccttgtcc
     1261 ttcggtaatt tcaggtgttc aaccgcgaat ttttccttgg tcaccagcac atggtagctg
     1321 aggcgttgta ctttctcgcc atacttcacg gtgacagcga gctcgtaggt gccgggggca
     1381 tcctgcatat cgatgccgac gagcccgaga tagccggacg gctcgcccgt tctcatttcg
     1441 cggaaaaaag gaattgtccg gtcgagaaag atgcctttga cgtcagtcgg ctcttcccgg
     1501 accgcaacct tcaccagcac cacctgacct tggcgcccgc tgtattgccc gtcaatgccc
     1561 ttgggcaagg gcggcgcatt cggaaacagt tcgaccggaa acacactagt cagcaacaca
     1621 atggtggtga tgagcatccg gtccagccga gaaccccgtt gactgacctg tttcaacgcg
     1681 ggaaggatca tctttccctc gaagctacga ctcggatgga ctacacgcta cacggtttgc
     1741 aacaagcccg gttgacggct tttcaacagc tctctcaccg gtaaaaccgc agaccggaca
     1801 cctgtgacac caactcatcg acccgtcgat tgaccgcact gaacggatcc atgcagagaa
     1861 tcgtttcctc ccagtagccg tttagcttca aataggtcca atccactgtg taggaccgat
     1921 tcttcgcccg ggcacatcta atgaagtccc cacgcacttt tgcccgcgtt gtttgcggcg
     1981 gtgtcgacat ggcccgctgc acggcgtctt cttcgaccac ccgctcgacc aatccacgcg
     2041 actccatgag gtagtacaac cccctcgtac gtttgacgtc atggaactgc aaatcaagga
     2101 gaaagacccg gggatcgtcc cagctgcagc ctttcttatc gacatatgac tggatcaggt
     2161 gccgtttcgt gatccaatct atttcacgca cgagctgcat gggatcctct tcgagcttgt
     2221 caagaatggt cgcccaccgc ttccagacat cgtccaaaat cgggtcatgg tcttgctgcg
     2281 tcaggtagtc atgcgcccgc tccagataca cgcgttggat ctcgaccgcc gtcatctgac
     2341 ggccgtcgtc aagcttgact ttgcgtttca ccgttgggtc gcgggagatt tcgcggatcg
     2401 ccttgacggg atcttccagc tccatgccgt gcacggaaaa accttcctcg atcatcgaga
     2461 gcacgagcgt tgcggtcccc accttgaggt aggtaaccag ttccgacatg ttggaatctc
     2521 cgacgatgat atgcagccgc cgatagcgtt ccgcatccgc gtgcggttca tcgcgcgtgt
     2581 tgatgatgct gcgcgaggac gtggtcgagg acgaggtttt ttcgtgtata tgttgcgcgc
     2641 gctgagaaat gaaaaactga ggtttgccgg aaaccttcag cactttccct gcgccgctgt
     2701 agatctgccg ggtcacaaaa aatgggataa gctgctcggt cactttccag aaatccacat
     2761 ctcgacgcat gagaaaattt tcatggcacc cgtaggtgtt tccgagtgaa tccgtgttgt
     2821 ttttgaaaat gtagatctct ccggaaagcc cttcttcacg cagccgttct tctgccgccg
     2881 gtaaacaggc ctccagcagg cgctcaccgg ccttatcgtg gacgacgaga tcgagaatgt
     2941 tgtcgcattc aggggtggaa tactccggat ggcagccggt gtcctggtag aaacgcgccc
     3001 cgttcaccag gaacgcattg gagggccaac tgttgggaat cagaccctca aagatatagc
     3061 caaggacttt ctccatgggc agatagatcc ggccgttcgg cgagaaaatg aggccatact
     3121 cattctcgag accgaagatc cgttgcttca ttggaccgga agatggcata agcggctcga
     3181 gacagaatac acggcctccc gctcatcttc aacagagcgg aaggattcag catgcaggat
     3241 tgattatgcc aggaactgct tcgtttcggc gggggtgtac cgacggaact ttcggttttc
     3301 tcgcgtgcgg tccagcacgg ccacttccaa cgtctcgagc gacagttttt gattccccgt
     3361 ctgctcaaga gccaggatgg agagattcag cgcagttttc agatcaggcg gctgagctgg
     3421 cactttttcc ttgagtgccg cctgcacaac ctccgatttt ccaccgatga cggcaaactg
     3481 cttctcatcg atgatgctgc cgtcgaaaga aatccggtac atctcgttgg tatgcccgtt
     3541 gtgcccgatc tggaccacga gaatctcgac ctccagtggc ttcatctcct ggctaaaaat
     3601 cgtcccaaga ctttgcgagt agccattggc cagcgagcgg gccgtgacgt cctcacggct
     3661 atacatgaag cccttgaggt ccgcatgcct gattccggcc ttgcgcaaat tttcaaattc
     3721 gctgtatttg ccggcaccgg cgaaggcaat gctgtcataa atttcagaaa ttttgtgcaa
     3781 actcgcgctc gggttctccg ccgcgagcaa aatgccgtcc acatattcca tggcaatgat
     3841 cgaacgcccc ttggcgatgc ctttcttggc atactccgcc ttgtcctgca tcatctgctc
     3901 gggcgagacg tagtagggca tcggcatgtc tatcgctcct tcgcgatgcg ggtcgccagc
     3961 atcgcctcgt atacacttcg gatgtcggat tcggacacgt cggtaatccc ctgagcgttc
     4021 acgatctttg cagtgggata aatccctctg atcaaatcgg ggccacccgt cccgacgtcg
     4081 tcatccgccg cattatagag ggcaaggagt gccaacttga gggcctcttg ttcggtcatg
     4141 gccttcttga agtgctcgcg catcgtgttg cgggcatcct ttccgccgga cccgatcgcg
     4201 tggtagtccg attcttcgta gcgcccgccg gccagatcgt atttgaagat gcgtccctcg
     4261 ccccgcttga ggtcgtatcc gacataaagc ggcatcacca ccagtccttg aaagaccatc
     4321 ggaaggttag ccttgaccat ctgcccaagc ttgttggcct tcccttcaca agacagttgc
     4381 atgccttcca gcttctcgta atgctcaagc tcggtttgga atagtttcgc catctcgatg
     4441 cacggccccg cggcaccggc gatcgccatg gctgagtagt cgtcgatctt gaagactttt
     4501 tcgatccggc gttcggcgat ctgaaatcct tctgttgcac ggcgatcccc ggcgatgaca
     4561 acgccttctt gtgatttgac cgccaacacg gtcgtcgcct gaggtacggt cacagggctc
     4621 accgaccgcg ggagctcgtg gtggaccggg agcggcggtt cggtcggccg gcgtcctccg
     4681 ggcgtcagtt cagggtgctg cctggtcagg aaatcaaaaa aactcgcgtc gtcgtggttg
     4741 ggaagaaagg gaagcttcat tatgctttca acttggagag caatatctct gccgtgtccg
     4801 attggtcgag caattcggcg gtcagagatt ctgtgccccg caagggatcc atcaacggca
     4861 ccttcttgat cgtggtattt cccacatcga agagcactga cgtccagctg gcgccataga
     4921 tggatttcga aaacttgccg atacaacggc cccggaagta ggcgcgggtg cccgaaggcg
     4981 gcgtgaattc ggcgcggatg acttcgacgt cctgcaccac ccgctcgatc agatggctcc
     5041 gctccagcgt atagtaaagt cctctgtcag gccggatgtc atgatactgc agatccataa
     5101 ggcgcacgcg tggatcgtcc cacccgcatc cctttcggtc catatacgac tcgatcatgt
     5161 gccgcttggc cgcccaatcg agttctccgg ccagcgaacg cggatctcgt tccagcttgt
     5221 ccagcacgtc ttcccaccgg acgagaacat ccttggtgac ctgatccaat tcatggcagg
     5281 cgtagaaatt catggcggac ttgagatacg cgcgctgaat ttccacggcg gtcgtcgaac
     5341 gcccgtccgt cagttttaac gtccccttca tcgtcaaatc gcgcgagacg ttcttgatcc
     5401 ccatgacagg atcgtccagt tcaatctcag ggacatccac cccggcctcg agaagatcca
     5461 ggacgattcc cagggtccca accttgagat acgtggacaa ctccgccatg ttcgcatcgc
     5521 caacgatgac gtgaagccgg cggtacttgc catagtcggc atgtggctca tcccgcgagt
     5581 tgacgatcgg ccgcttgacc atcgtattga ggtcaaccag gcattcgaag aaatcggccc
     5641 gctgagaaat ctgaaattca gtgaaactcg tttgattttc tgcgccgact ttgccggcgc
     5701 ctgcatagat cagtcgggtg acgaagaagg gagccagcgt cttcacgatc cgttcgaatg
     5761 gcaccgaccg tgagagcata tagttttcat gataaccgta actgtttccc ttgccgtcgg
     5821 aattgttctt gtacagcacg aattggtctc gacctctgag tcgtgccatg ttgtcgaggc
     5881 attgaccgag aatccgctcg ctaatacgct cgtacgccac cacttcgcga gggtttgtgc
     5941 attcgggcgt ggagtattcc ggatgagcgc catcgacata cagtcggccg ccgttggcaa
     6001 gcaccttgtt caattgccga ttgtaatctg ggttcggccg ttcccgctcg ccctccactt
     6061 caaaccctcg cgcgtcgagc agcgggttct cgttctcata gtcccacacc gcatgaggaa
     6121 ctgaaatgcc ggggtaatgt ccgatgaccg cgagtgaatt tgatatcgga tcgacggcag
     6181 aggcatcccg tgaggcaatg ccgaattccg tttcggttcc cagtacgcga ggcgtcgtcg
     6241 ggggtgtgga gtcccgcatg gggggttaga gataatggcc ggtggtgata gtttcgatct
     6301 gacgggactc ggccgggccg ccgctgatcg tcctgatgtg cacgatcttc tcacctttct
     6361 taccggctac tttcgcccag tcatccgggt tagtcgtatt gggcagatct tcgtgctctt
     6421 tgaactcttc gcgaatcgcc cggatcaggt cgtccgaccg aaggccacgg ccttcctggg
     6481 caagagcccg cttgaccgca tacttcttgg cgcgcgacac gatgccttca atcaaggcgc
     6541 cgctggcaaa atccttgaaa tatagaatct ccttctcgcc attggcgtag gtcacttcga
     6601 taaatttgtt ttcttcggag gtcgcataca tggctcccac cgtcaggtcg gtcaaatgtt
     6661 cgaccaacgc cttggtatcg cctccgtgac
//
LOCUS       DASTQY010000238         9256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_432274, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000238 DASTQY010000000
VERSION     DASTQY010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432274"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1432)
                     /locus_tag="VFR82_07825"
     CDS             complement(47..1432)
                     /locus_tag="VFR82_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU4501544.1"
                     /translation="MLHIRFHDGLLVRLLKFQAGFLGLLLCFAPAPGLSATLTESDTA
                     SQLAEAPPVINSTDEPAVDPADEPDANLVPVETELPPDSAASAASQPESRVPRAEDLL
                     VVTPPQQSSTARFTDAFDAPAETAETVPSLGGLMEPSELYNVPIVMDSSVQSHIRYFN
                     TSIRNRFEQWLLRLSRYRPLVENIFAEFHLPSDLVFLSLVESGFNPYAYSRAKATGPW
                     QFMKGTAKVYGLRVDAYVDERRDPIKSTVAAARYLRDLYDLFGAWPLAMAAYNAGEGK
                     VMRALQKAQAESFTDISKTRLIRRETKEYVPRFMAATIIAKNPNRYGFPQETSAPHQF
                     EEVVVTRPLHFHAIANATGIPFSELRLLNPELRRDATPPGDDAYLLKIPVGTKAKVEQ
                     LLDRVPTYKFPREAKPQFASAGSRWYKVRVGDTLEKVSRRFRIPLKTLKTRNNLTGPT
                     IRPGDFLVIGR"
     gene            1676..2818
                     /locus_tag="VFR82_07830"
     CDS             1676..2818
                     /locus_tag="VFR82_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEU4501545.1"
                     /translation="MLKRYLLAPGPTPVPPEVLLAMARPMIHHRAPEFDPIFAEAREG
                     LKWLFQTRNDVLMLAASGTGGMEGSVSNFLSPGDKALCINGGKFGERWGKICKTFGVQ
                     AIELKVEWGHAINPQLVKDALKKDPAIKAVYVQASETSTGVSHDVKALGEIVKAHDQA
                     ILVVDAITALGVFDIKTDAWGLDVVITGSQKALMLPPGLAFVSVSDKAWALADKAKNA
                     AFYFNFKKERENQVKNQTAFTPTVSLIIGLQEVIKMLKAEGLDKIFARQGQLAQAMRE
                     GMKAAGLALFPKESPSDALTAVSAPDGVDGQAVYKNLRVQYGITAAGGQDHLKGKIFR
                     LSHMGYADRFDVITAVAATEMVLKGLGHPLKLGSGVAKAQEILMAK"
     gene            2828..4411
                     /gene="serA"
                     /locus_tag="VFR82_07835"
     CDS             2828..4411
                     /gene="serA"
                     /locus_tag="VFR82_07835"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEU4501546.1"
                     /translation="MKILVSDSLSTQGVELLERAGFTVVVKSKMPKDELLREIKDADG
                     LIVRSGTKVTEELIIAAEKLKVVGRAGSGLDNVDTPAATRRGIVVMNTPGGNTVTTAE
                     HTMSMICAMSRRIPQATTSVKAGKWEKDKFMGVELFNKVLGIVGVGQIGSHLTKLAQG
                     VGMRVIAYDPYLAADRAEKMGVEMVSIAELFRRADIISVHTPLTPETKNLINAQAIGT
                     MKPGVMIVNCARGGIIHEGDLYEALKSKRVAAAAFDVFEEEPVKADHPLLSLDNFICT
                     PHIGAQTTEAQENVAVGIAEQIVDYFTKGIARGAVNIPSVSPELLPRLQPYLSLAEQL
                     GRLETQLCEGGLERVTVEYSGDVTTLSIAPLTIAVLKGLLTPIMEAPVNYVNAPIVAK
                     ERGIEVKEVKSTDAGDFTSLIRVRVEAGKQSRLVAGTLYHKKEPRVVEIDQFKVEVVP
                     EGHMLFIHNIDRPGVIGMVGKVLGDNNINIVRMQCALEKRGGNALLIIGSDQAFPAHV
                     LNTIQSSKDIVSVKVAALS"
     gene            4468..5868
                     /gene="hisZ"
                     /locus_tag="VFR82_07840"
     CDS             4468..5868
                     /gene="hisZ"
                     /locus_tag="VFR82_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEU4501547.1"
                     /translation="MVTAPKSRLSSGHSTSSRERSLVPVGMATILPHAAKQVRRIEAQ
                     LLAQISSWGYDEIILPTFEYLDVLAPGLEAELLETCYQFIDRTTGRTLLLRPDATAQV
                     ARTIGMGLTGSTVPQRLSYRTSVFRYEPEHAGRGREIFQVGAELVGVDDVTGDYEIIS
                     LLIECLQAIGLRSFTIALGHVGFIKGLLMRSGLSPQGQKRAEQAAAKKDLPRLEELLA
                     SERIAKAAAKAIREAPELTGQEEVLERGRILAAGEPTLTAPLDRLAQVYDLLCAAGHR
                     DSLLLDLGEFRGFDYYDGVVFDVFASGVGAELGGGGRYDHLIGRFGRDLPSTGFALDV
                     DHLFRAVEMPQSGVDSPRLDYVVAGLRRSMRTVIEIADTLRRTQRRVVQALIKGSEHT
                     LLSNALEAAQAQGAKALIVIGAPGMAQDHVAYFEKLGGADGRRNGRPHRPAMKKLTRA
                     ALLAGERSRRRRKESK"
     gene            5865..7220
                     /gene="dnaB"
                     /locus_tag="VFR82_07845"
     CDS             5865..7220
                     /gene="dnaB"
                     /locus_tag="VFR82_07845"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU4501548.1"
                     /translation="MKSMPGIDLSQPKLPPQNVEAEQSVLGAILLDNQAMAKAMELVV
                     EENFYRTAHRKIYRAMLELSETGEVIDQITLTERLKAQGELEAIGGAAYLAELVQMVP
                     SSANIRYHSKIVRDKALLRELLHTSTEVLTRGYEGTAPIDELLDFAERSVFSIAQGKL
                     DRSFSPINAIIKESLDLVDKLSKRKEQVTGVPTGFYDLDDLTAGLQPSDLVVIAGRPS
                     MGKTSLVLGMAAHAALHAGTSVGIFSLEMSKPQIVLRMLSSEARVDSHALRTGKLQKE
                     DWWRLAEAAGRLEQAPIYIDDTGGITVQQMRGKARRLKAERGLDLLVVDYLQLMQGRS
                     DAESRQQEISDISRSLKALAKELNVPVVALSQLSRAVEARKPPIPMLADLRESGAIEQ
                     DADVVMFIYREDVYDQNSERKGIADILVSKHRNGPIGKKELFFHDRFAKFESLDNREV
                     V"
     gene            7354..9102
                     /locus_tag="VFR82_07850"
     CDS             7354..9102
                     /locus_tag="VFR82_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4501549.1"
                     /translation="MPLSFLAAAACASPSKSPERPVQTAQPAKPASSARPADASAAYH
                     FMLGYQAELSQNSEQALKEYEAALKADPNALSVKARLASLHFSLGDMPNALRYAEEVA
                     DGRAEDARMLTHMAGILAGGGKGDKALSVLDRAVELDPESGDAYFSKGLLLLNLKRPA
                     EAEQAMRAGIARSPDSAVGHYQLGRVLLEAGKVEEATTSFERAITVNYSFEPAYLALA
                     AIYESRHEKERAVGVLKRYLQQVNSRNRDIRHQLVRLYIDAKDYSGARKELEAMIAED
                     PSDLDAQLRMALIHGEQKEYPQAIERLTEILKVRPAELKVRDYLGYVYEESKDTQKAL
                     ETYTFNVQLEPTFFEGHLHLGVLYYRLKKFPEATEHLGRAIALNPKQPESHIVQGLAY
                     LQQDQYDKSAEVFQEGIRHNPKNADLHFNLGTAYDKLNRFDDVVRAMETAIRLDPHHA
                     DALNYLGYSYADRGIKIDQAISLTKQAVALKPENGYYVDSLGWAFFKSGLLTEALVEL
                     KRAAALVGDDPVIYEHLGDIYAKQQRLSDAREAWLHALELDPSNHKLMDRFREQGMGD
                     PAQEERIQQAIRRVAG"
BASE COUNT         2061 a         2557 c         2774 g         1864 t
ORIGIN      
        1 tgcgtcggag acacacccaa tgccgttcaa gagggcgcgc gcctttttag cggccgatga
       61 cgagaaagtc gcccggtctg atcgtcgggc cggtgagatt gtttctggtt ttgagggtct
      121 tcagcggaat gcgaaagcgg cgggagactt tctccagggt gtcccccacg cgaactttgt
      181 accagcgaga gccggccgaa gcaaactgcg gtttcgcctc ccgggggaac ttataggtgg
      241 gaacgcgatc gaggagttgt tctactttgg ccttcgttcc gactggaatt ttgagcagat
      301 acgcatcgtc gccgggcgga gtcgcatcac gacgaagctc aggattgagt agtcggagct
      361 cggagaacgg gatacctgtg gcgttcgcga tggcatggaa gtgcagcggc ctggtgacca
      421 cgacttcctc aaattgatgg ggcgcgctgg tctcttgggg gaaaccgtag cggttcggat
      481 tttttgcaat gatcgttgcg gccatgaacc ggggcacata ctctttggtc tcacgacgga
      541 tcaaccgggt ctttgagatg tcggtaaagc tctcggcttg agccttctgc agcgcccgca
      601 tgaccttccc ctcgccggca ttgtaggccg ccatggccaa cggccaggcg ccgaacaaat
      661 cgtacaaatc gcgaagatag cgggctgccg cgacggtcga cttaatggga tcacgccgtt
      721 cgtcgacgta cgcatccacg cgcaagccat agactttcgc cgtgcctttc atgaactgcc
      781 atggtcctgt ggctttcgcg cgcgagtagg catacggatt aaacccgctt tccacgagcg
      841 acaagaagac gagatcgctc gggaggtgga attcggcgaa aatattctcg accaagggtc
      901 gataccggct cagccggagc agccattgct caaaacggtt tcggatggag gtgttgaaat
      961 accgaatgtg actttgcacc gatgaatcca tgacgatggg cacattgtag agttcgcttg
     1021 gctccatcag gccgccgagg gacggcaccg tttccgccgt ttcagcgggg gcatcgaatg
     1081 cgtcggtgaa gcgcgccgtg gatgactgct gtggcggcgt gacgaccagc agatcttcag
     1141 cccgcggcac gcggctttcc ggctgcgatg ctgctgatgc tgcactgtca gggggcaatt
     1201 cggtttcgac ggggacgaga ttggcgtccg gttcgtctgc aggatcaacg gccggctcgt
     1261 cggttgaatt gatgaccggc ggcgcttcgg cgagttgaga tgccgtgtcg gattccgtca
     1321 gcgtcgcgga gagtccagga gccggtgcaa aacagagtag gaggccgaga aaacctgctt
     1381 gaaacttgag cagcctgacc aagagaccat cgtggaatcg aatatgcaac atgtgtgaag
     1441 ctagactatc cataggcggg aaagttgtca aggaattgcg gcgggaacga ggccatcctt
     1501 tcgtaggacg cctccccgca gccgttgcat cctcacatcg ccgtagattc ctcaacacat
     1561 cgaaatcacg atcgtcagcg tcggtcgttg ccttgacagt cttcggaggg cggtgttatc
     1621 gtcggccctt cccagagccg cattccccgc atttccattc caggaggagt cgtcaatgct
     1681 caagcggtac ctgctagccc ccggtccaac tcctgttcca ccagaagtgt tgctggccat
     1741 ggccaggccg atgattcacc accgggcacc cgagttcgac ccgatctttg ccgaggcgcg
     1801 ggaggggcta aaatggctgt tccaaacgag aaacgacgtg ctcatgctgg ccgcttccgg
     1861 cacaggtgga atggaaggct ctgtttcgaa tttcctgtcg ccaggtgaca aggcactctg
     1921 catcaacggt ggaaaatttg gagagcgctg gggaaagatc tgcaaaacat tcggcgttca
     1981 ggccatcgaa cttaaagttg agtgggggca tgcgatcaat ccgcaactgg tgaaagatgc
     2041 cctcaaaaag gatcccgcaa tcaaagctgt ttatgtgcag gcaagtgaga cctcgacggg
     2101 cgtctcgcac gacgttaagg cattgggcga gatcgtgaag gcccatgatc aggccattct
     2161 ggttgtcgat gcgatcaccg cgttgggcgt gttcgatatc aagaccgatg cctggggact
     2221 cgatgtcgtt attactgggt cacagaaggc attgatgctg ccgcccggcc ttgccttcgt
     2281 gagtgtgagc gacaaagcat gggcccttgc ggacaaggcc aaaaatgcgg ccttctattt
     2341 caatttcaag aaggaacgag agaatcaagt caaaaatcag actgccttca ccccgacggt
     2401 ctcattgatc atcgggcttc aggaagtgat caagatgctg aaagccgagg gcctcgacaa
     2461 gattttcgcc cgccagggac aattggccca agcgatgcgg gaaggcatga aagcggcagg
     2521 ccttgccctg tttcccaaag aatcacccag cgatgcctta acggccgtgt cagcgcctga
     2581 cggcgtggat ggacaggcgg tctataagaa tctaagggtc cagtatggca ttactgccgc
     2641 cggtggacag gaccatctta aaggtaagat ttttcggttg tcccacatgg gatacgccga
     2701 ccggttcgac gtcattacag cagttgccgc cacggaaatg gtcctgaaag gactcgggca
     2761 tccgctgaaa ctcggaagtg gcgtggccaa ggcgcaagaa attttgatgg ccaaataggc
     2821 aggcacgatg aaaattctgg tcagcgacag tctctcgacg caaggcgtcg aacttctcga
     2881 acgggcaggc tttactgtcg tggtgaagtc caagatgccg aaagacgaac tgctcaggga
     2941 gatcaaggat gccgatggac tgatcgtgcg ctcgggcacg aaagtgaccg aggaactgat
     3001 tatcgccgct gagaagctga aggtcgtcgg ccgcgctggc tctggcctgg acaacgtcga
     3061 tacgccggca gcgacccgtc gcgggatcgt cgtcatgaac acaccgggcg gtaacaccgt
     3121 gacgacggct gaacatacga tgtccatgat ctgcgcgatg agccgccgca tcccgcaagc
     3181 gacgacgtcc gtcaaggcgg gcaagtggga aaaagataaa tttatgggtg tcgagctctt
     3241 caataaagtg ctcggcatcg tcggtgtcgg gcaaattggc agccacctga cgaaattagc
     3301 ccagggcgtc gggatgcgcg tcatcgccta tgatccctat ctcgcggccg accgcgcaga
     3361 gaagatggga gtcgagatgg tgtcgattgc cgaactgttt cgacgggccg acatcatctc
     3421 cgtccatacg ccgttaacgc ctgaaacgaa aaatctcatt aacgcgcagg cgatcgggac
     3481 gatgaagccc ggggtcatga tcgtcaactg cgcacgaggc gggatcatcc acgaaggcga
     3541 tttgtacgag gcgctcaaga gcaagcgtgt ggcggcggct gcattcgatg tgttcgagga
     3601 agaaccggtc aaggcagacc accccctgct gtccctcgac aatttcatct gcacgcctca
     3661 catcggcgca cagacgaccg aagcgcagga gaacgtggcg gtgggcatcg cagaacagat
     3721 cgtcgactat tttacaaagg gcattgcccg cggagcggtc aacattccgt cggtgtcgcc
     3781 ggaattgctg cccaggctgc agccgtatct ctcgctggca gagcaactcg gcaggctgga
     3841 aacgcaactc tgcgaaggcg ggcttgaacg ggtcacggtg gaatacagcg gcgatgtgac
     3901 gactctctcc attgcgcccc tgacgattgc cgtcttgaag ggtttgttga cgccgatcat
     3961 ggaggcgccg gtaaattacg tcaatgcacc catcgtcgca aaagagcggg gtatcgaagt
     4021 caaggaagtg aaaagcaccg atgcgggcga tttcacgagc ctgatccgcg ttcgcgtgga
     4081 agccggcaag caatcccgcc tcgtcgccgg caccctctac cataagaagg aaccgcgggt
     4141 cgtcgaaatt gaccagttca aagtcgaagt cgtcccggaa gggcacatgc tgttcatcca
     4201 taatatcgac cgcccggggg tcattggcat ggtcgggaag gttctcggtg acaacaacat
     4261 caacattgtc cgtatgcaat gtgcgcttga gaagcgtggc ggcaacgcct tgttgatcat
     4321 cggctcggat caagcgttcc cggcgcatgt gctgaacacc attcagtcga gtaaagatat
     4381 cgtgtccgtc aaagttgcag ctctgtcctg aacgttccaa gtctgaatgt tccaagccgc
     4441 cacctacacg cgctccgtcg gttaatcatg gtcactgctc cgaagtcgcg cctctcttca
     4501 gggcattcga cgtcctcccg tgaacgttcg ctcgtccctg tcgggatggc aaccattctg
     4561 ccgcatgccg ccaagcaggt ccgccgcatc gaggctcaac tacttgcgca gatttccagc
     4621 tgggggtacg acgagatcat tctgccgaca ttcgagtacc tggatgtgct ggctcctggt
     4681 ctcgaagccg agctcctgga aacctgttat cagtttattg accgcacaac cggccggacg
     4741 ctcttgttgc ggccggacgc gacggcgcag gtggctcgaa cgatcggcat gggcctgaca
     4801 ggaagcacgg tccctcagcg gctctcctac cgaacgtcgg tattccgcta tgaaccggaa
     4861 catgcgggga ggggtcggga aatttttcaa gtgggagccg agctggtcgg agtcgacgat
     4921 gtcaccggtg attacgaaat catcagtctc ctcattgaat gtttgcaagc gatcggcctg
     4981 cgttcattta ccatcgccct cggtcacgtc ggattcatca agggactgct catgaggtcg
     5041 gggctctctc ctcaggggca aaaacgtgct gaacaggcgg cggcgaagaa agacttgccg
     5101 cggctcgagg aactgttggc gagcgaacgg atcgccaagg ctgcggccaa ggccattcgg
     5161 gaagcgcctg aactgaccgg acaagaggag gtgttggagc ggggacgcat tctcgctgcc
     5221 ggcgagccga cccttacggc gccgctcgat cgactggcac aggtgtatga cctcttgtgc
     5281 gctgcaggcc atcgcgattc cttattgctc gatcttggcg aattcagggg gttcgactac
     5341 tacgacggcg tcgtgttcga cgtgtttgct tccggcgtag gcgctgaact gggcggaggc
     5401 gggcgctacg atcatttgat cggcagattc gggcgcgatc tcccctcgac cggattcgcc
     5461 ttggacgtgg atcatctctt ccgcgccgtc gagatgccgc aatcgggagt cgacagtccg
     5521 cgccttgatt atgttgtggc tggtcttcgt cgatccatgc gcacggtgat cgagattgcc
     5581 gacaccttac gccgcacgca gcgccgagtg gtccaagcgt tgatcaaagg cagcgagcac
     5641 acccttctgt cgaatgcgct tgaggccgca caggcccaag gcgccaaggc actgattgtc
     5701 atcggtgcgc cagggatggc tcaggatcat gtggcatact tcgaaaaact cggcggggcg
     5761 gatggacggc gcaacggacg tccgcatcgt ccggccatga agaagctcac gcgggccgct
     5821 ctattggcag gtgaacggtc gcggcgacgg cggaaagaat cgaaatgaaa tccatgccgg
     5881 gcatcgacct ctcgcagccg aaattgccgc cgcaaaacgt cgaggcggag caatcggtcc
     5941 ttggcgctat cctgttggac aaccaagcca tggccaaggc catggagttg gtggtcgaag
     6001 aaaatttcta tcgcacggct caccgcaaaa tctatcgtgc catgctcgag ctctctgaga
     6061 ccggagaggt catcgatcaa atcacactaa cggaacgact gaaggcccag ggggaacttg
     6121 aagcgatcgg cggcgcggcc tacttggccg agttggtgca gatggttcca agttcggcca
     6181 acatccgtta tcacagcaag atcgttcgcg acaaggcgtt gctccgtgaa cttctgcata
     6241 cgtcgacaga agtcctaacc agagggtacg aaggaacagc gcccatcgat gaactgctgg
     6301 actttgccga gcgctcggtc tttagtatcg cccagggaaa acttgatcgg tcattcagtc
     6361 ccatcaacgc gattatcaag gaaagccttg atctggtcga taagctttcc aagagaaaag
     6421 agcaggtcac gggcgtcccg acggggttct atgatctcga cgatctgacc gcgggactgc
     6481 aaccttctga tctggtggtg atcgccggcc ggcccagcat gggaaagacc agtctcgtcc
     6541 tgggtatggc cgcacatgcg gcgctccatg ccgggacatc ggttggcatc ttcagcctgg
     6601 aaatgtcgaa gccccagatc gtgcttcgca tgctcagttc cgaagcgcgc gtcgattccc
     6661 atgcgctccg caccgggaaa ctgcaaaaag aggactggtg gcgactcgct gaagctgcgg
     6721 gacgtcttga gcaagccccg atctatattg atgataccgg aggcattacc gtgcagcaga
     6781 tgagggggaa ggcccggcgg ttaaaagccg aacggggatt ggatctcttg gtcgtcgatt
     6841 atctgcagct gatgcaggga cgaagcgatg ccgaatcgcg tcagcaggag atttcggaca
     6901 tctccaggtc gctgaaggcc ctggccaagg agttgaacgt gccggtcgtt gccctctcac
     6961 agctcagtcg ggcagtcgag gccaggaagc cgccgattcc aatgctcgcg gatctgcgtg
     7021 agagtggtgc gatcgagcag gacgccgatg tcgtgatgtt tatctatcgc gaagacgtct
     7081 atgatcagaa ctcagaacgc aaaggcatcg ccgacattct cgtcagcaag catcgcaatg
     7141 gtcctatagg gaaaaaggaa ctttttttcc acgaccgatt cgcaaagttc gaaagcctcg
     7201 acaatcgtga agtcgtttga ccctgccatg accgcttcgt ataatcggtg agtgtttgcg
     7261 agtcctctca ggattgctgt tgatcgtccg atgagacggt tgaaattgta tagcactaca
     7321 acagcctggt cgcttcgatg tctttgcgcg ttgatgccgc tctccttcct ggcggctgcg
     7381 gcctgcgctt cgccgtcgaa atcacccgaa cggccggtcc aaaccgccca acctgccaag
     7441 ccggcatcgt cagcccgtcc agctgacgcg tctgcggcct accacttcat gctcggctat
     7501 caagccgaac tgtcgcaaaa ttccgaacaa gccctcaagg agtatgaggc ggcattgaaa
     7561 gccgacccca atgctctttc cgtgaaagca cggctggcca gcctgcattt ttcgctcggc
     7621 gacatgccaa atgccttgcg ttatgcggag gaggtggccg acggccgggc cgaggacgcg
     7681 cgcatgttga cgcacatggc gggcattctt gccggaggcg gcaaaggcga taaggcgttg
     7741 agcgttttgg atcgcgcagt ggaactcgat cccgaatcgg gagacgcgta tttctccaaa
     7801 ggcctcttac tcctcaatct taagcgcccg gcggaagcgg agcaggcgat gcgggccggg
     7861 atcgccagat cgcccgatag cgccgtgggg cattatcagc tgggacgggt attgctggaa
     7921 gcggggaaag tggaagaggc gaccaccagc tttgagcggg ccatcaccgt caattactcg
     7981 ttcgagccgg catacctggc gctggccgcc atttacgagt cgcgccatga aaaagagcgc
     8041 gcggttggag tgctgaagag atatctccaa caagtcaact cccgcaaccg agacatccgt
     8101 caccagcttg tgcggctcta tatcgacgcc aaggactatt cgggtgccag aaaagaactt
     8161 gaagcgatga ttgccgaaga tccgtctgat ctggatgcgc agctacgaat ggccctcatc
     8221 cacggcgaac agaaagaata ccctcaagct atcgagcggt tgacggagat tctcaaagtc
     8281 cgtccggcag aactgaaagt gagagactat ctcggctacg tctacgagga gtcgaaagac
     8341 actcaaaaag cgctcgagac ctatacattc aatgtgcagc tcgagcccac gtttttcgaa
     8401 gggcatttgc atctaggcgt gttgtactac cggctcaaaa agttccctga agcgacggaa
     8461 cacctgggga gggcgatcgc cctgaatccc aaacagccgg aatcccacat cgtgcaagga
     8521 ttggcctacc tgcagcagga tcaatacgac aaatctgccg aagtgtttca agagggcatc
     8581 cgtcataatc ccaagaatgc tgatttgcat tttaatctcg gaacggcgta cgacaagctg
     8641 aaccggttcg acgatgtggt gcgagcgatg gaaactgcca tccggcttga tccgcatcac
     8701 gccgatgcgc tcaactacct cggatacagc tacgcagatc ggggaattaa aatcgaccag
     8761 gcgatttcac tgacgaaaca agcggtcgcg ctcaaacctg agaacggcta ctatgtggac
     8821 agcctcggct gggcattctt caaatcaggg ttattgaccg aggcccttgt agaactcaag
     8881 cgggcggccg cgctggtagg agatgatcca gtgatctacg agcatctcgg cgacatctac
     8941 gccaaacaac agcgtctctc cgatgcccgc gaagcctggc ttcacgccct ggaactggat
     9001 ccgagcaatc acaagttgat ggaccgcttc cgtgagcaag gcatgggtga tccggcacaa
     9061 gaagaacgaa tccagcaggc cattcgcaga gtcgctggct agaaatcctc cctgacggtc
     9121 gcgcaataac gagcgaacca gtcattttct tgcgatcatc ttcaatcctg ctagactcct
     9181 gccgttgtgg tgcaccacca tgccattacg gagcccgacg agatatcggg cggccagcgc
     9241 cctgcaggtg cgctcg
//
LOCUS       DASTQY010000239        16297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1411774, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000239 DASTQY010000000
VERSION     DASTQY010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 16297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16297
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411774"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1164)
                     /locus_tag="VFR82_07855"
     CDS             complement(70..1164)
                     /locus_tag="VFR82_07855"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU4501550.1"
                     /translation="MDVTGIMKPTSSRVYLVLLHVMFLVLYGYHLVWPMTRVQGGLII
                     ALSVLTLLVLTVPRTQLGTGWFLGLLTLGNASILFTTTMIDKVGTGPWILCALMLLLA
                     MASYVTCLFHFALLNGLVIGSYALLLHQMSVLQTENVLLLPAFLCLTLVFLSKISITQ
                     AEIRRLTETEEYTRNKSMCDALTGLPNRAQFLERVGRAVQCLQQNRDLHFALLFVDLD
                     GFKPINDRLGHKAGDTVLRQTARAFQGCLRKGDIVARYGGDEFTFLLNNVKGPSDAIR
                     VAERILAKVHTPIDVGEPVQVGASIGIALSTNMHERPEELIRDADGAMYRAKARGKNC
                     FVLSDQVSDVSKSELKERWKRIAHLGLNRV"
     gene            1426..1782
                     /locus_tag="VFR82_07860"
     CDS             1426..1782
                     /locus_tag="VFR82_07860"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4501551.1"
                     /translation="MQNKQTKAGPVPDQDKAERRGRRLPINRRLFFFGEEEFEGEATL
                     LDISTNGCRATSLTEVKIGILLKLSLFLQDQQWPLRVDQAVVRWVEGQCFGLEFISIR
                     PAQRERLRAILMKAKA"
     gene            2697..3104
                     /locus_tag="VFR82_07865"
     CDS             2697..3104
                     /locus_tag="VFR82_07865"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU4501552.1"
                     /translation="MAEFMHPYASQTYALMRIVTGFLFLWHGAQKLLSIPTPPPPQVP
                     SFVIAIAGPIELIGGTLVMIGLFTGWAAFLCSGLMAFAYWMGHGTQALLPIQNMGELA
                     ALYCFVFLYISSRGSGIWSVDAARKQPSGVSKH"
     gene            4381..4791
                     /locus_tag="VFR82_07870"
     CDS             4381..4791
                     /locus_tag="VFR82_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4501553.1"
                     /translation="MLHAKTPSIRIILVDDHPSFRQGLRSLMDSYPDIEVVGEAENGE
                     EVVHSMPYICPSAIVMDINMPKLNGIEATRTIKAHYPHVVIVGLSVHADSLYQTAMVA
                     AGAKSVVSKGMAGLFLYREIKAGVENSPPVPNAA"
     gene            5084..6919
                     /gene="nhaA"
                     /locus_tag="VFR82_07875"
     CDS             5084..6919
                     /gene="nhaA"
                     /locus_tag="VFR82_07875"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEU4501554.1"
                     /translation="MTDHARPVKLDRPVDAKQDHVLGNPHAEITLVEYGSYACRHCSA
                     MHEVIADLRDRMGDRMRYVFRHRPIAGSEDSQRAAELAKYVSTTTGQFWGAHDALMKQ
                     GTTFSDGDFERIASQFGLPLRDEKDEKGSSTAAANVAEDIESARRSGVLETPTFFINN
                     RRYEGPWDESSLAEAMLGSLGHRLHAATVDFVRWAPSAGFSLLLMSLLALLLVNSPIG
                     PAFQSWWDKSLAVQLDGRTFGLPLLQWVNDGLLSVFFLVVGLEIKREFTVGRLATRHA
                     AALPVVAAFGGMTVPALIYLLVVPSGPLQAGWGMTMATDTAFAVALMVLLGTRVPVEL
                     RVFLTAAVIIDDLVAILVIALFYTDHLDVSYLVASGAVTALLAALNQWGVYRSLPYAV
                     LGVVLWICLHGSGLHATLAGVILAIVTPTRPPANLHALLAQAEAVIRVETKRRDEGVM
                     QHGPSEPALQALDVIHDRIESPASKLLRSVEPWSSYVVLPVFALANAGVVWSLAILEG
                     HGRLMLGIILGLVIGKPIGMFTTAWLAAHLGIAVKPAAYSWRQLFGAGALAGIGFTMS
                     LFIAGQAFSHPEDYAAAKVAVFVASLMAGVAGVLILWPRTTEEAG"
     gene            complement(7023..8492)
                     /gene="cls"
                     /locus_tag="VFR82_07880"
     CDS             complement(7023..8492)
                     /gene="cls"
                     /locus_tag="VFR82_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU4501555.1"
                     /translation="MYSAWIMDHLGTILTAALALYALGTGAFLILENRRPQATLAWML
                     AFIFAPGIGLLAYMLFGRDRKAFSKQSELLRQDLETNALPLLNPMLSRQDAEIARLER
                     ASTGRRKLMRLVRHNSYSALTKRNCVEIQQDAAVFYPSLIRDMESAQHSIHLQYFIWG
                     VDPFTDGLKGIMKRKAKAGVEVRLLYDPIGSQAHIGRAYVRDMRAAGIRMAPTSPLYR
                     FHTISYRNHRKITVIDGAIGYTGGMNIGQEHIDGGQGFDSWRDTQVRIVGESVALLQT
                     VFMVDWYNAVKENLFAAAYFPADAMACSTGDVPVQILTSGPDSQWAGIRQLYSFMIVS
                     AQRHVRIQSPYFIPDATLAEALKTAALSGVDIKIMLTARPSGNRLPDWAGNTYIADIV
                     ASGVRVFLYEKGYLHAKTISIDSEICSIGSTNIDIRSFSINYELNAVLYDEQLAKQLE
                     QDFERDLTHCSEFDRTSYEKRNVAVRFRDSVARLLSPLL"
     gene            9130..9813
                     /locus_tag="VFR82_07885"
     CDS             9130..9813
                     /locus_tag="VFR82_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4501556.1"
                     /translation="MRILLVEDDLNLAQFILKGLREEGYAVDSAADGEEGLELAFANP
                     YDLVILDIMLPKLDGLTLCRNLRTAGNMTPVLLLTARDTIEDKVSGFDIGADQFLTKP
                     FAFAELLARVRSLLRRGGAQQPTRLTAADLHLDPASRKVTRSGKDIILTNKEYALLEF
                     LLRNKNRVLTRTAIIEHVWDISYDPMTNIVDAHIRALRAKIDREFSPPLIVTVRGAGY
                     MLEEPKPVE"
     gene            9810..11249
                     /locus_tag="VFR82_07890"
     CDS             9810..11249
                     /locus_tag="VFR82_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4501557.1"
                     /translation="MKSLRGLIRQSALILIAGLLLVFSILIYAGGDALLRRFVDARLL
                     GMAETVAKIVEQYPNFIKSPVEDGVVSAEVSRSEKEQLQLSGVTHSLRVFSPEGVLVW
                     KGSDAEPPRPITEQVFVQARLGHVVVETVESVDGTPVRRLFLPIVQEGKVQYVLQAEA
                     SLRLYRETLKGLVLLLTMGSATILLVAWLGSSWLAKKVLNPLDVLSTEAETMSESDLG
                     KRLILGSPYQEFRRLTQAFNSVMDRFQRSVESQRRFVDHAAHEMQTPLTVLQGNLEVA
                     LLKARTQEEYREALINNLEQVGSLISLTRSLLTLAKFTGDTPPVQLAPLPLGPLIRDI
                     LAELSILFDERRIILSFESQPVPNVYGDAQWIKQAVINLLDNALRYTPSGGKVTVRLQ
                     TVGKWVAVIVEDTGHGIEPEHLPHLFERFYRTDWARAKDSGGTGLGLPIVKGIAAAHG
                     GRIAVTSQVNKGSIFTLRLPIPIQETVPA"
     gene            11541..12185
                     /locus_tag="VFR82_07895"
     CDS             11541..12185
                     /locus_tag="VFR82_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HEU4501558.1"
                     /translation="MNLKGLVHRELGEGVTEKELAAAAGVSVRTIGNILADKLPQDPE
                     IWETFARYFRIDADVLRAGGSPLPEGVFELADSNHRVRVGSMRTVPLLNCHQVDQMVT
                     SNEPPRVVHAEAMLETDVPGKRTFAVTVKDDSMQPLFSEGEIIFVNPDLLSAPGHYVM
                     VGSEDGNPEEALLRQLKDIGGQVVLHALNRRYEDLPFTKQHRIWGRVVRLRKNL"
     gene            12238..13047
                     /locus_tag="VFR82_07900"
     CDS             12238..13047
                     /locus_tag="VFR82_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501559.1"
                     /translation="MRKKMIWQLAVLVLAIGGVSSASVAGPPSGSEQGHPQLLPGDRI
                     LIGTVVEVRGDQARIDTGEGQPRFVPMGVREVKGLPDLKTGDGVEITVNEQNLLVDVH
                     LIGEGSHHRVVEGHLVQPLVTRHDRAVLRTTQGKEESHAIRPVARSKMASVSPGVIAV
                     FLIDESDRIVDVTFGNMEAVRRAAELWGKKTPLKGNFDRVIGTILTPLENNRIAIRTE
                     EGEEQPYEVRPLAQLGVVKLSYGDAVILLVDDEGKVTGVAIPPRSSESDRG"
     gene            13244..13375
                     /locus_tag="VFR82_07905"
     CDS             13244..13375
                     /locus_tag="VFR82_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501560.1"
                     /translation="MGDTIILIVSFPLIIAGMILTITWGMSAAGAGGQGGRKQTKEA"
     gene            13443..14081
                     /locus_tag="VFR82_07910"
     CDS             13443..14081
                     /locus_tag="VFR82_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501561.1"
                     /translation="MSLIHHSIAALALAVVGLAGCAHDTRAVGEANPVHVADTKAAMR
                     DLWTGHIFWIRNVVSDNAVHNAAARDAAEQEVVANARSIANSITPFYGEKASEKLFSL
                     LAGHYGAVKEYSEATVSGNRLHQDAALTHLASNTDDIAVFLSGANPHLPKDTVRGLIA
                     AHGAHHVLQITQFKERDYVHEGETWKVMKQHVYVIADALTMALATQFPSKFS"
     gene            14721..15524
                     /locus_tag="VFR82_07915"
     CDS             14721..15524
                     /locus_tag="VFR82_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501562.1"
                     /translation="MRRNRFLRLAILTVCWVLPVSVGHGASGSDHADSHTFPGNRVLL
                     GTVEEVRSEQARIDTGEMSPRFIPMGVRMAKDLPALKKGDRVEIIVNDQNLLVDVHLV
                     GESSYHRVVEGQLVQVLVTGHGRAVFRTTRGQEESHAIRPVARSKMASIPVGVDAVFL
                     IDEMDRIVDVTFGNKEAVHRAAELWQKKTPLKGNFDRVTGMFLTPLDNNRITIRTEEG
                     SNQLYEVRTLAQPKLAKLAKGDAVVLLVDDEGKVTDVAIPPRSSESDQR"
     gene            15588..16019
                     /locus_tag="VFR82_07920"
     CDS             15588..16019
                     /locus_tag="VFR82_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4501563.1"
                     /translation="MNTTGVPVGGLKTVGQIVATNTLTFQAGQNGLAIAVELLSTRTA
                     GAPVVDSEGKYLGFINEFDVMKALSLGRELSSLVAEDLMRIGPIAVHSSTTIADAAQR
                     MDEQCVLNLPVEKDGRVAYSVSRHDLLRAWIGLGLGMGLDP"
BASE COUNT         3759 a         4229 c         4583 g         3726 t
ORIGIN      
        1 tgtttaacag ttctggagcc ggacgatgca gtttccataa tccgcaaaga atactcgcgt
       61 ccttcctgac tagacccggt tcaaccccag atgcgcgatc ctcttccagc gttctttgag
      121 ctcacttttt gaaacgtccg agacttgatc gctgaggaca aaacaatttt tcccccgagc
      181 ctttgcacgg tacattgcgc cgtcggcatc acggatcaat tcttcaggac gttcatgcat
      241 gttcgtgctc agggcaatac cgatgctcgc gccgacttgc accggttcgc cgacgtcaat
      301 aggcgtgtgc acttttgcga ggatgcgctc agcaacgcga atcgcatccg agggaccttt
      361 gacgttgttc agtaagaacg tgaattcgtc tccaccatac cgtgcgacga tgtcgccctt
      421 cctcaagcac ccttgaaacg cccttgccgt ctgtcggagc acggtgtcgc cagctttatg
      481 ccccagcctg tcgttaatag gtttgaaccc gtccaagtcc acaaacagga gcgcaaagtg
      541 caaatcgcga ttttgctgca gacattgcac ggcccgaccg acacgctcca ggaattgcgc
      601 gcgattcggc aaccccgtga gcgcatcgca catggattta ttgcgagtgt attcctccgt
      661 ttcagtcaga cgccgtattt ctgcttgcgt aatgctgatc ttgctcaaga acaccagcgt
      721 gagacacaag aaggcaggca ggaggagcac attctcggtc tgcagcacgg acatctgatg
      781 cagcagcaac gcatagctgc cgatgaccaa accattcaac aaagcaaaat ggaacagaca
      841 cgtgacatag gaggccatcg cgagcaagag catcaaggca cagaggatcc aggggccggt
      901 gcccacctta tcgatcatcg tggtggtaaa aagaatgctc gcattgccga gggtcagaag
      961 accaagaaac catcccgtgc ccagctgagt tctggggact gtgaggacca aaagggtcag
     1021 aacggacagc gcgataatca gtcctccctg tacccgtgtc attggccaca caagatgata
     1081 gccgtagagc acgaggaaca tgacgtggag cagtacgaga tagactcgtg agctggttgg
     1141 cttcatgatt cccgtcacgt ccataatccc cctcccgcct cgtacacaaa gcctacccgg
     1201 tgctcccgga agagacattc ccaggaaggg taggaagaag gcgtactaac gtagggagga
     1261 ggggatatga gaaagtactt agcttcggtg cgctgactgg tcgtcctcgt gtgtctgctg
     1321 cgaccaggcc attggaatta gtactcttga ggaatgctta taatggattg atcgaatacc
     1381 attgagtgag aacagtgagt ctcgtggctg gggaatgaag acactatgca gaacaagcaa
     1441 acgaaagcgg gtccggtgcc tgatcaagac aaagcggaaa ggcgtggtcg ccggctgccg
     1501 atcaatcgcc gactcttctt tttcggcgag gaggaattcg agggcgaagc caccttatta
     1561 gatatctcaa ccaacggctg ccgtgccacg tcactcactg aagtcaagat cggcatactg
     1621 ctcaaactgt cgctatttct tcaagaccag caatggcctt tgcgcgtcga tcaggcggtg
     1681 gtgcgatggg tagagggcca gtgctttggt ctggaattca tcagcattag gcccgcccaa
     1741 cgcgagcgat tacgggccat cctcatgaag gcaaaagcct gacagtgctc gccatgacct
     1801 cctccggctc ttttctcctg ctggatgatg gggacataaa ttaatccgtt ggttcaatcc
     1861 ccggatcatt tcggcctcat gcggctcaac gacaggccgc tctcttcggc cgaagccgca
     1921 actatcggct atcaattcac catcctgcgc gtaccgtgtg gccggctctg ctcaggtcaa
     1981 cgctgcaggc gaaaaccgtc tgggtttcca gacctccgct cctcaagtcg ccgctgtgaa
     2041 tccgcgagct tggcgaccgc gttggcgtaa aagcagattc gtttgagatc ctcgttcgag
     2101 aacggcatat ggtcagacgc ttgtgcgcta tctaatagcc gctcgcagta acggaaaaaa
     2161 ccatgaaggt cttcgcggat ggacggaggt ggcgagtgag acataggacg accgcctttc
     2221 acggtgtctg gcggtcgggc cttcccagga ggccaggcac aggtatcggg tggaatgaat
     2281 caatctaggc tctcttgaag accgggtcaa gcggtcattc gaactggagt aatagtgatt
     2341 gttgaaagga cgagttcgaa tcccgtccac catgaataca ttcgccttat aggtgaagtt
     2401 gaaaccgact ccgcagtctt gacgcacact gaccgcaaaa tttgaagccc tccaaacaac
     2461 ctcgccaatc gcctcgtcgg gcttaccagc ttctttacgg tatctaccca gcgctgtatg
     2521 gcgggcctga ccatgtgtgg gatttatcta gtacaagatt caaaagggga aaggcacgcg
     2581 gccatattgg gcaatggccc gaagcgtgtt gacagcctgg attgaaccca gtagtcttcc
     2641 ctagttctga gtgccacgtc cttcatgggc atccacctgt gtagaaggag gattcgatgg
     2701 ccgaattcat gcatccgtat gcctcccaga cctacgcgct catgcgaatc gtgacgggat
     2761 tcctcttcct gtggcatgga gcccaaaaac tgctgagcat tcccacccca ccaccgcctc
     2821 aagttcccag cttcgtgatt gcgattgcag gtccgatcga actcatcggc ggaacgttgg
     2881 tcatgatcgg gttgtttaca ggatgggcgg catttttatg cagcggcctc atggcgttcg
     2941 cctattggat gggtcatggc acgcaggcgc ttctccctat tcagaacatg ggtgagctgg
     3001 ccgctctgta ctgcttcgtc ttcttgtata tttcttcgcg aggctcaggc atctggagcg
     3061 tggacgccgc acgaaagcag ccttctggcg tctcaaagca ctagatagca tctggagata
     3121 acctgggagt aaaccccgcg ctggggacgc caggctttcg ctcgacggca tacaaaaatc
     3181 actgaataaa gaagccccga tccttttcca gcgtcccgct atagcgaccg tgccggctac
     3241 ggctttaaaa aatcactgta tggatctgac gcaggctggc gttcgtcgaa acgatactgt
     3301 ggcccgactg gtcgttgaaa gcagacttac aaggaggcga aataccggtt aacgacgact
     3361 cgtgattgcc gtcgcttttg ctagctcgat tagtggaaac gacctttgag taggcatcag
     3421 attccatacc gtgtggagcc cgatgaaggg gaggattggt cctcattgag ccatgtaaag
     3481 gccgcttcaa aatcacggaa ggctttcagt tgatatcctc tattcatcgc gcaaagctca
     3541 agaaacgctc ctctatcaaa atcgtccttc ggcctattca ccactgcgac ctgagccctg
     3601 agccctgagt tcgtcagctt ccttgccaga gtccacacgt ccagcatgct ggcattcgaa
     3661 gtcgcttctc gagtatcgat gagaatccgg tctacattgt gctttttgca ttcttccacc
     3721 aaatcacgca gtatgatgag actcctctcc aaatccaggt tgccggacac atctgttcgc
     3781 aggaattctt tgatagggat tattctcact tcgacaggca tagttcaccc cggtgttgta
     3841 cagtcgatcg ccgccacggt aaatcatcga ttcgccgcag catagtagat agcaaacagt
     3901 ctgggatccc aacgaatgaa tgatgtctct atagctgatt caacaggggg accgcaacct
     3961 cgtgaagcta atcctgttaa agctaataag gcacgcttgt cgcatcccca ctactgggca
     4021 agaccgataa gaagatcaag cccttctcca taaagccaaa agacagcgaa agcattctat
     4081 tttacccgtc cgacatacac tccactcttg ttccagggtc acgtgcatgg cggttgcccg
     4141 gtcaggtaaa ggcagcctaa aatccctagt agaaaaaggt ccctcattcc agggagggac
     4201 gaggctgatt aaggagaagg ggtcatgcta aagttgcaga aactgggcca caccgaaaat
     4261 cagccgctgg tcagatcgtt ctgaagaaga ttggcggttt taaaccctgc ggagaataca
     4321 ttgtagatcg catcaccaga attctccaac gatcctcacg ctcgcgaagg atgtgtgaga
     4381 atgctgcacg ctaaaacacc ttccatccga attatccttg tcgacgatca cccgtcattt
     4441 aggcaaggtt tacgatcttt gatggactca tatccggaca ttgaagtcgt cggcgaggcc
     4501 gagaatggtg aggaagtggt ccattcaatg ccctatattt gtccttccgc gattgtcatg
     4561 gacatcaaca tgcccaagct gaatggcatt gaagctaccc gtacaatcaa ggctcattat
     4621 cctcacgtgg tgatcgtggg gttatctgtt catgcggaca gcttgtatca aactgccatg
     4681 gtcgcggccg gcgcgaaaag cgtcgtctcg aaagggatgg cgggtctatt tctgtatcgg
     4741 gagatcaaag ccggggtaga gaattcaccg ccggtgccca acgctgccta aaatagtcat
     4801 ttcttcgtct ttgctgatac agcaaggagc cctcccaagt gcattcaaga gtagactggc
     4861 tatagcacga gcacactttc aacatccctc tcagtgggtt catcaagcaa aaagcggact
     4921 catttcaata aactgaaacg cgggccttac agttggacat tgtcgttgat cgaccccttg
     4981 aatcggcaag gcataagtcc caccatatga ggaaatcctg tagcgaagag ttttaccgag
     5041 tgcgcgcctg attttccgag ggatcactca ctgtcaaacg ccgatgacgg accacgccag
     5101 accagtcaaa ctagatcggc cggttgatgc caagcaggac catgttctag gcaatcctca
     5161 tgccgagatt accttggtgg agtatggtag ttacgcctgc cgtcactgtt ctgccatgca
     5221 tgaagttatt gccgatctgc gcgaccgcat gggggaccgt atgcggtatg tgtttcggca
     5281 tcgaccgatt gcaggcagtg aggattccca gcgagcagcg gaattggcga aatacgtgtc
     5341 gacaacgacc ggccaattct ggggggccca cgacgccctc atgaaacaag ggacgacgtt
     5401 cagcgacggg gatttcgagc ggatcgcgtc acagttcggc ctcccgcttc gcgacgagaa
     5461 ggacgagaaa ggtagttcca ccgctgcggc gaatgtggcg gaggacatcg aaagcgcacg
     5521 ccgcagcggc gtgttggaga cgccgacctt cttcatcaac aaccgccggt atgaggggcc
     5581 gtgggacgag agctcgctgg ccgaggcgat gttgggttcg ctcggacacc ggctgcacgc
     5641 tgcgacggtg gatttcgttc gatgggcgcc atcggccggc ttttccctgt tgctgatgtc
     5701 gctcctcgcc ctcctgttgg tcaattcgcc aatcggcccg gcgttccagt catggtggga
     5761 caagagtctt gccgttcaat tggacgggag gacgttcggt ttgcccttgc tgcaatgggt
     5821 gaacgacgga ttgctgagcg tttttttcct cgtcgttggc ctggagatta agcgcgagtt
     5881 cacggtgggc cgcctggcga cgcgccacgc ggcggcatta ccggttgttg cggcattcgg
     5941 cggcatgacc gtcccggcgc tcatctactt gctcgtggtc ccatcgggac cgctccaagc
     6001 gggctgggga atgaccatgg ccacggatac tgcctttgcc gtcgcgctga tggtcctgct
     6061 cggaacgcgc gttccggtag aattgcgcgt ttttttgacc gcggcagtca tcatagacga
     6121 tctcgtggca atcctcgtga tcgctttgtt ctacacggac cacctcgacg tctcttatct
     6181 ggttgcctcg ggtgccgtga cggccttgct ggcggcgctc aaccagtggg gggtgtatcg
     6241 ttcattgccc tatgcagtac tcggtgtcgt gctctggatc tgtctgcatg gctcgggact
     6301 tcatgcgacg ttggccggag tgatcctcgc cattgttacg ccgacgcgtc ctccggcaaa
     6361 cttgcatgcc ctgttggcgc aggccgaagc cgtgattcgg gtcgagacca agcggaggga
     6421 cgaaggcgtc atgcagcatg gaccgtcgga accggccctt caggccttgg atgtcataca
     6481 tgaccggatt gagtctccgg caagcaagct gttgcgttct gtcgaaccgt ggtccagcta
     6541 tgtcgtgctg cctgttttcg ccctggccaa cgccggtgtg gtctggtctc tcgctatact
     6601 cgaagggcac ggacggttga tgctgggtat tattctggga cttgttattg ggaaacccat
     6661 tgggatgttt accactgcct ggctcgccgc tcatctcggc attgccgtga aaccggcggc
     6721 ctattcatgg cgacagctgt ttggcgccgg cgcacttgcg ggaatcggct ttacgatgtc
     6781 gctcttcatc gcaggtcagg ccttctctca cccagaggac tatgctgccg caaaggtcgc
     6841 cgtctttgtc gcttcattga tggcaggagt cgcgggcgtc ttaattctct ggccgcgaac
     6901 gactgaagaa gccggttagc aggcggtcct cacttctggg ctgtaccagc gatggctgtg
     6961 acggtcaagc agaggaaccg cagcagttct gagcgtgagg gcggatcgcg gatcaatccg
     7021 attcacagta acggcgagag aagcctggcg accgagtcac ggaatcgaac ggccacattg
     7081 cgcttctcat aggaggtgcg gtcgaattcc gaacagtgag taagatcccg ctcgaagtcc
     7141 tgttcaagtt gtttggcgag ctgctcgtcg tacaataccg cgttcagttc gtaattgatg
     7201 ctgaagcttc ggatgtcgat attggtcgag ccgatggagc agatctcgga atcgatactg
     7261 atcgtctttg cgtggaggta gcctttctcg tagagaaata cgcgtacgcc gcttgcgacg
     7321 atatcggcga tatacgtgtt gcccgcccaa tccggcaacc ggttgcccga tggccgcgcc
     7381 gtcagcataa tcttgatatc gacgccagac agggcggcgg ttttgagagc ttcggccaga
     7441 gttgcgtcgg ggatgaagta cggcgattga atgcgtacat ggcgctgggc cgagacgatc
     7501 atgaaggagt agagctgcct gatccctgcc cactgtgaat ccggtcccga cgtcaaaatc
     7561 tggacgggga catcgcctgt cgaacaggcc atagcgtccg cggggaaata ggcggcagca
     7621 aataagtttt cctttacggc attgtaccaa tcgaccatga acaccgtctg taagagcgcc
     7681 acgctttccc cgacaatgcg cacctgggtg tcgcgccacg aatcgaaacc ctgcccaccg
     7741 tcgatgtgct cctgcccaat attcatgcca cccgtgtagc cgattgcgcc atcgatgacg
     7801 gtgatcttgc ggtggttgcg atagcttatc gtgtggaacc gatagagggg tgacgtgggc
     7861 gccattcgga ttcccgccgc tcgcatgtct ctgacatagg ctcggccgat atgggcctgg
     7921 gagcctatcg gatcatagag cagacggacc tctacacccg ccttcgcttt cctcttcatg
     7981 attcctttca agccgtcggt aaatgggtca accccccaga taaagtactg gagatggatg
     8041 gaatgctgtg cagattccat gtccctgatc aggctcggat agaacaccgc cgcgtcttgc
     8101 tggatctcca cgcaatttcg cttggtcagc gcggaatacg aattgtgccg caccagacgc
     8161 atgagttttc ggcggccggt gctcgctcgt tcgagccgag cgatctccgc atcctgacgg
     8221 gacagcatgg gattgagaag gggaagggcg ttcgtttcca gatcctgtcg aaggagctcg
     8281 ctctgcttac tgaaggcttt ccggtctcta ccgaacagca tgtaggccag caagcctatg
     8341 ccgggagcga agatgaacgc gagcatccag gctaaggtcg cttgtggccg ccggttttcc
     8401 aaaataagga acgccccagt gccgagcgcg taaagagcca gcgcggcggt cagaatcgta
     8461 ccgagatgat ccataatcca tgcgctatac atcgctaaag gtccgtgcgg gatagaccat
     8521 attctgcatc attcaaaacg agttacggct aagcttcaag tactagcgac tgcgtctgaa
     8581 tacctctgat catcaatcag attagaacat cttcacgatc tcttcatagt atgttcatgc
     8641 agcgtgagca taatacggtc atcacaaaaa gcgctggcag aaagggcaac gcatattcga
     8701 acgtggagac cttgatgatg agaagaggga acgacacaat gatggaaacg attgcgatat
     8761 atctccttgc ttttatggtt ctgttctcgc tcgtattgct gatggtgtgg gggttctcaa
     8821 tcacggatat gtcgtcgtcg actttcaact cctagttaac aatcgaaggg gagtcgatga
     8881 tgatcggagt gggcatgaat tgtcggaacg gttcgggcag gtttacacta ctcctcctta
     8941 acaatttgcg atgagagata aagggagcag tcaatcattg cacctgctgc aagcttggat
     9001 gtgtattctc ctgacggtgc aataatcaag gctggaacgg cgtcattgtt aagcttgatt
     9061 ctcaatccga ttcgtggaca tgattcacct agtactgttc ccagaaatag cccatggggt
     9121 gaagcggtca tgcgaatcct ccttgtcgaa gacgatctca atttggctca attcattctt
     9181 aaggggttga gggaagaggg ctatgcagtc gactccgcgg cagatggaga agaggggctc
     9241 gagttagcct tcgcgaaccc ctatgatctt gtgatcctcg atatcatgtt gccaaaactt
     9301 gatggcctca cactctgccg gaatctacgc actgccggaa acatgacccc ggtgttgtta
     9361 ctcacagccc gggatactat agaagacaag gtatctggat tcgatattgg tgcggaccaa
     9421 ttcttgacca agccttttgc ctttgcagaa ctgctagccc gagtccgctc tttgctcagg
     9481 cgaggcgggg ctcaacagcc gacgcgtctt acggccgcag atctccacct ggatccggct
     9541 tcacgcaagg tgactcgctc tggcaaagac atcatactca ccaacaaaga atatgcctta
     9601 ctggagttcc tgttacgaaa caagaaccgg gtgttaaccc gcacggcgat catcgaacac
     9661 gtgtgggaca ttagctatga tcccatgacc aatatcgtgg atgcacatat ccgcgccctg
     9721 cgcgccaaaa ttgaccgaga gttttcgccc ccactgatcg tgaccgtacg cggcgcgggg
     9781 tacatgctag aggaaccgaa acccgtcgaa tgaaatctct gcgtggtctg atccggcaat
     9841 ctgcgctgat tctgatagca ggactcctcc tcgtcttttc cattttgatc tatgccggcg
     9901 gcgacgcctt attgcgccgc ttcgtcgatg ccaggctgct gggaatggcc gagacagtgg
     9961 ccaagattgt tgagcaatat cccaacttta ttaagagtcc cgtagaggat ggtgtcgttt
    10021 ctgccgaggt gagccgaagc gagaaagaac agcttcaact gtctggagtt acccattctc
    10081 ttcgcgtttt ctcgccagaa ggcgtgctcg tgtggaaagg gtccgacgca gagccaccgc
    10141 ggccgattac cgagcaggtg tttgtacaag cccgtcttgg gcatgtcgta gttgaaaccg
    10201 ttgagtcggt cgatggcact cctgttcgtc gcctgttctt acccattgtc caggaaggaa
    10261 aggtgcagta cgtgctgcag gccgaagcat ccctgcgact gtaccgagag acactcaagg
    10321 gactcgtgct tctattgacg atgggatcgg caaccattct tcttgtggcc tggctaggta
    10381 gcagttggct ggccaagaaa gttctcaacc ccctcgatgt gctaagcacc gaagcggaaa
    10441 cgatgtccga atctgatctc gggaaacgac tgattctcgg ttcaccctat caagaatttc
    10501 ggcggcttac ccaagcattc aactccgtga tggaccgatt ccagcggagc gtcgaaagtc
    10561 aacggcgatt tgtcgaccat gccgctcacg aaatgcagac gcccttgacc gtgttacagg
    10621 gcaatttaga ggtcgcgctt ctcaaagcca ggactcagga agaatatcgt gaagcactga
    10681 tcaacaatct cgagcaagtg gggagtctga tctcgttaac ccgttcactc ctaacgctgg
    10741 cgaagttcac gggcgacacc cctcccgttc aactcgcgcc gctcccgctc ggacctttga
    10801 tccgcgacat cttggctgaa ttgagcatcc ttttcgatga gcggcgaatc atcttatcgt
    10861 ttgaatccca accggtgccc aatgtctacg gtgatgcgca gtggatcaaa caggcagtga
    10921 ttaatctgct cgacaatgct ctccgctata caccttctgg tggaaaggtg accgtccggt
    10981 tgcagacggt cggaaaatgg gtcgctgtaa ttgttgaaga cacgggtcat gggatcgagc
    11041 ctgagcatct tcctcacctc ttcgagcggt tttatcggac cgattgggcc cgcgcaaaag
    11101 actcgggtgg tacgggtctc ggactcccta tcgtgaaggg aatcgcggca gcccacggcg
    11161 gaaggattgc ggttaccagt caagtcaata aaggttccat ctttaccctg cgtttgccca
    11221 tccccattca ggaaacggtt cctgcctagt tcggcagcac tccgagcttc ttaacctctt
    11281 cctttctctt cttctatgaa aaaatcttca tgctccgttc atggaacaat catgtgcacc
    11341 agttagagtc tcatcaacga atgaatctcg tatccgacaa ggtgaattat ggattgagac
    11401 aacgcggagc gaatcgtgtc ggcctcgaga aaatcggtaa atggccaggt tgcccaagat
    11461 tcgctcccgt ttgtgtatgt ggggtgaagg actggtggga cctctaagaa gtcggcttta
    11521 ggtaaagaag gaagaacgtt gtgaatctga aaggtcttgt acatcgagag ctcggcgagg
    11581 gagttacgga aaaagaattg gccgctgcag ctggagtctc cgtgcggacg atcggcaaca
    11641 ttctggcgga caagcttccg caagatccgg agatctggga aacctttgct cggtattttc
    11701 gcatcgatgc ggatgtgctt cgagccggcg gttcgccgct cccagaggga gtgttcgaac
    11761 tagcagacag caatcatcgc gtacgagttg gctcgatgag gacagtgccg ctcttgaatt
    11821 gtcatcaggt tgatcaaatg gtcacgagca atgaaccgcc tcgtgtcgtc catgcggaag
    11881 ccatgcttga gacagatgtt ccggggaagc gcacgtttgc cgtgacggtc aaggacgact
    11941 ccatgcagcc cctttttagc gaaggggaga ttatttttgt gaatccggat ctcctgagtg
    12001 ctcctggtca ttacgtcatg gtgggaagcg aggacggcaa cccggaagag gcacttctcc
    12061 gacagcttaa ggatatcggc gggcaggtag ttcttcatgc gctgaaccgg cggtatgaag
    12121 atctcccctt cactaaacag catcgtattt ggggcagagt cgtacgcctg cggaagaatc
    12181 tttgaacatc agacaggcat tgggtttctc agaatcgaga attgaaagga gaagcacgtg
    12241 agaaaaaaga tgatatggca actggctgtc ttggtattgg caattggagg agtctcatcg
    12301 gcatccgttg ctgggcctcc ttccggttcc gagcaagggc accctcaact acttcccggg
    12361 gatcgcattc tcataggcac cgtagtggaa gtacgagggg atcaggcacg gattgatact
    12421 ggtgaagggc aaccgcgatt tgttcctatg ggagtgcgag aggttaaggg gttgccggac
    12481 ttgaaaacag gagatggggt ggaaatcacc gtcaatgaac aaaatctgct ggttgatgtg
    12541 catctgatcg gtgagggaag ccaccatcgt gtcgttgaag gccaccttgt tcaaccgttg
    12601 gtgacgcgac atgatcgggc ggtgctgcgc acgacccaag gcaaagaaga gtctcacgcc
    12661 atccggccgg ttgcacgaag taagatggct tctgtttcac cgggtgtgat tgccgtgttt
    12721 ctcatcgatg agtcggacag aattgtggac gtgaccttcg gcaatatgga agcggtgcgt
    12781 cgcgcagcag aactctgggg gaagaaaaca cccctgaagg ggaacttcga cagagtgatc
    12841 ggtacgatcc tgacgccatt ggagaacaac cggattgcta tccgaaccga agaaggggaa
    12901 gagcaaccgt atgaggtgcg tccacttgct caattgggcg tggtgaagct gtcctatggt
    12961 gatgcggtga tacttttggt ggacgatgaa ggcaaagtga cgggcgttgc gattccaccc
    13021 aggtcaagcg agagcgatcg agggtagagg ttggctcacg gcataacaat tcgtctaaag
    13081 gctcttaatg cgaatcaccg gtttgcgggc aagacggttc ggaagatgaa ggtttcttca
    13141 tggcccgttc acggccattt catcttacgc tgttacattg accgtgaaac agcgagtggc
    13201 tggttagaac aggatttcaa gaccgaggag gggaggtgcc accatgggcg ataccataat
    13261 tctgatcgtg tcgtttccgc ttatcattgc agggatgatt ctgacgatta cgtggggcat
    13321 gagcgctgca ggtgcgggcg ggcagggcgg gcgcaagcag accaaagaag cgtgagaacc
    13381 gacaccttct aggagaaccc cgctgcccca tatcaatgag gtccaatata aagggagggt
    13441 ttatgagcct gatacatcac tctatcgcag cactagcact cgcggtcgtt ggcctggcgg
    13501 gctgtgcaca cgacacgcgg gcagttggag aagccaatcc ggtccacgtc gctgacacga
    13561 aagcagcgat gcgcgatctg tggacgggac atattttctg gattcgcaat gtcgtttcgg
    13621 acaatgcggt acacaatgct gcggcccggg atgctgcgga gcaggaagta gtggccaatg
    13681 ccagaagtat tgcaaatagc atcactccgt tctatggcga gaaagcatcc gagaaactct
    13741 ttagtttact cgccggccat tatggtgcag tgaaggagta ttcggaagcg accgtctcag
    13801 gaaacaggct acatcaagat gccgcactga cccacttggc ttccaatacc gacgatatcg
    13861 cagtgtttct tagcggagct aatccccacc ttccaaagga taccgttcgc ggtctgattg
    13921 ctgcacacgg agcacatcat gtcctccaga tcacgcagtt caaggagagg gattatgtgc
    13981 acgaagggga aacgtggaag gtcatgaaac aacatgtcta tgtgatcgcg gacgcgctga
    14041 caatggcgct ggcaacccag ttcccaagca agttttcata actgagcgag gaatcgcaaa
    14101 ggctcgcctt cgtcgaaaag ctaaaggaag gcccgtggtg ccaacagccc gcgggccttc
    14161 gtattcgagc ccgtaaaatg acacgaagcc ctacgaatgc gatcgatgaa gaattcttca
    14221 tgatccattc attgcttcat catctgactg gactatagtt aggagcgaag ggaggttgaa
    14281 tatgaaacgc accatggact caccaaagcg aaactggatt aatgcttgga ctgaaagtgg
    14341 taatggccct agcgtgtggt tgcttcccgg aggtggccag ttctacacaa gatcgtgcat
    14401 tcgttgtgcc cgtttacttc ctaagacatc gagccatgat cggagagaca accgcgtcaa
    14461 ttctgagagg atgcgctgcc tccattgtgg ggatcctatt gattcgacga tccttcgaac
    14521 cagaacgcgc cgcgcgattc gttcttcatt agaaccatga accatccgcc tccttttctc
    14581 cgctttgctg actggttgaa atgaacctaa tccgtggggg gaaatgaggg acttatctag
    14641 cagaccgcac gaatagacag gagcagccta ttacaaggag tagaacctct aagaattgct
    14701 caaatcgaaa ggatagcaac atgagacgca acagattctt acgcttggcc attctgacag
    14761 tctgctgggt tctgccggta tctgtcggtc atggggcatc cggttctgac cacgctgaca
    14821 gtcacacatt tcccggcaat cgggttcttc tcggcacggt agaggaagtt cgaagtgaac
    14881 aagcgagaat tgacactggc gaaatgtcgc cccggtttat tcccatggga gtacgaatgg
    14941 cgaaggatct gccggcactc aaaaaagggg atcgcgttga gatcatcgtc aatgatcaaa
    15001 atttgctggt cgatgttcat ttggtcgggg aatcaagcta ccatcgcgtc gtcgagggcc
    15061 aactcgtcca agtgctcgtg accggccatg gtcgtgctgt gtttcgcacg actcgaggcc
    15121 aagaagagtc tcacgctatt cggccggtcg cgaggagcaa gatggcgtcc attccggtcg
    15181 gtgttgacgc ggtgttcctg atcgacgaga tggatcgaat cgtggacgtg accttcggta
    15241 acaaggaagc cgttcatcgc gcagcggaac tgtggcaaaa gaaaactcct ctgaagggca
    15301 acttcgacag ggtgaccggc atgtttctaa caccattgga caacaatcgg atcaccattc
    15361 gaaccgaaga gggtagtaat caactatatg aggtgcgtac gctcgctcaa ccgaagctcg
    15421 caaaattggc gaagggtgat gcggtggtgc ttttggtgga cgatgaaggc aaagtgaccg
    15481 acgtggcgat tccgcctcgg tcgagcgaga gcgatcaaag ataatggccg cggactcttc
    15541 cgcgactttg acataacgag agttggaatt cacttgaagg aggattcatg aacacgacgg
    15601 gtgtaccagt tggtggacta aaaacagtag gtcaaattgt ggccacgaac acgctgacgt
    15661 ttcaggcggg gcagaatggg ttggcgattg cggtggagtt gttgtctaca cgcacggcgg
    15721 gggctccggt ggtggattcc gagggtaagt atctgggttt catcaatgag tttgatgtga
    15781 tgaaagcgct ttcgttaggc agggagctaa gcagcctcgt agccgaagac ctcatgcgga
    15841 tcgggcctat tgcggtacat tcatccacga ccatcgcaga cgctgcccaa agaatggatg
    15901 agcagtgtgt gctgaatctt cctgtcgaga aggatgggag agtggcctat tcggtgagtc
    15961 gccacgatct gctgcgagcg tggatcggac tggggttggg aatgggcctc gacccgtaaa
    16021 gaaaatgccg tgctaggacc ggcccagaca gcttccattt ccgcctgtca tggtttgatg
    16081 tagagccgga atgccaccga catcgcggac gcaaaattgt gactctttct aaccatgtta
    16141 caagtccttc ggcctcgcta tgggtcatgc ctcttggccg gacaccgcca taccttgatc
    16201 cgcagagcca tgaagccgta tatgaggtcg atgtgagtgg gatgcggatt atgttctctt
    16261 ctcatcggca tcagagttaa ccaaggaatg gatgagt
//
LOCUS       DASTQY010000240         5454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_571452, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000240 DASTQY010000000
VERSION     DASTQY010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571452"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFR82_07925"
     CDS             complement(<1..200)
                     /locus_tag="VFR82_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501564.1"
                     /translation="MARTIVILHPGSLGDLLLAVPAIRRLRLRYPHHRLVLCAHGQAA
                     ALLGATRLVDEWIAFQGAGCAAL"
     gene            complement(206..823)
                     /locus_tag="VFR82_07930"
     CDS             complement(206..823)
                     /locus_tag="VFR82_07930"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEU4501565.1"
                     /translation="MIADVTGVLLAGGKSQRMGEDKRFLSIGEETLYAHSLTVLRSVF
                     QTVVVVIAQDSPPLESEVPVWRDLIPGCGSLGGLYTGLKQALTPSVFAVACDMPFLDP
                     VTVRYFLTVQGEADLVMAKLENRLQPMHALYHRRCLPVMDSLIQAHELKIQQLADHPS
                     LRVRLVTADELVGIDPAGRSFYNVNTPADLLAARSLHEKILPESS"
     gene            complement(914..2689)
                     /locus_tag="VFR82_07935"
     CDS             complement(914..2689)
                     /locus_tag="VFR82_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU4501566.1"
                     /translation="MMNSSSGKAGVVAVAAFGIILASGSVSVPLIGAEDMPEGFKKGE
                     LAPEPSAEMIEAGKRVYFTKCVWCHGVEGAGDGPGADRLWPRPRNFNQGTFKIRHTAS
                     GELPLFDSRKPVPGQNDLFETVTHGLPGSAMPSWEGILTEEQRLQVLSFVTTQLVKDR
                     KFTDKQSEAQTVLQLGDIKPIAATEESLKKGSELIVEKKCVECHGAEGRGDGNAFNLK
                     DDWGFAIQPADWHKCWNFRGSRQDPYNVRNIFRTFSTGVNGTPMPSFADNTTVEERWH
                     IANWVNFLCERDTDGKPLPIDPLTDKPKINFVVPSDPVEGEIPTDMENEAWQKASKRY
                     VAMGGQITHKPRNFVNRIDDIWVRSLYNDKFIVYLLEWDDRTKSVAEGKLPWAPTQVN
                     LDIKEQAPKTGEEGSIASNQNNYKVYNDAIAIETPVKWKELPAPIKPRYLFGTNEQFP
                     VDIVKWEADGSIRAFKGTGWDKDFEERDTYEENLKVLKGEWKNGRWYVMIQRPLGNKK
                     DQDYDEDTFFEVGQYVPTVFFAWDGHNGDAGRKMAVSAFYYTFLNPPIPKETYIYPAI
                     IAFGVVLLEGWVMTRRANKKKGKTL"
     gene            complement(2741..3778)
                     /locus_tag="VFR82_07940"
     CDS             complement(2741..3778)
                     /locus_tag="VFR82_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU4501567.1"
                     /translation="MKALMSVGALVGVVGLLLLVGMIFDVVPSNTVRLVEGYMPMQML
                     FELTLFVAGFAGLSYMMSSMGMPLPRFWQGIGFWAFILLYLKYRVYPPIPFSVRAMYG
                     TVSLVAVFMWVSSNEEDWKKFKQPILNVLDAQSGGNKVLRYAYLILLPILIGGFSYNA
                     MKPKSEEPIELRTVHPAPPASTKVHGKTYVLQTSQNPYRVNPEGKYDQEFTNANIVEQ
                     GMGRLMKPNANPWDEKSTGYLKYVREGGEIFFQNCHFCHGDNLNGRGLHAFAFNPIPA
                     NFTDPGTIAQLQETFIFWRVAKGGIGLPNEGFPWASVMPPWEQHLTVDEIWKVILFEY
                     WHTGYYPRTWD"
     gene            complement(3817..5130)
                     /locus_tag="VFR82_07945"
     CDS             complement(3817..5130)
                     /locus_tag="VFR82_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU4501568.1"
                     /translation="MGNLINEALSMGWVVLALLAGLLIYFQVSISDPAAKKRATFKTF
                     IGIIAAILMFIAVANYKNNFYGENRLLPVSLVMITVTTFVMAIYFTNLAALLKIGGFM
                     FFVAAFLSGYGNWLPQVEGGFPPKEEKLDFNSMSPQQLADEGEKIIFGGIGQNKVQGA
                     IGKGQCPLCHAFHAGMLGERAPNLLGLPERAGKERLEDPKYSKGNPAKREYAEKESCP
                     GCGTAENGQEYIAESHSCPSCYVVSGYGVKGTNDKESPMPKIHKPPISLSLPELAAVD
                     TWLYVREGREAPAFEEITKSYEKFIPEAERPKQQEDKPAGAASALLADGSEPVDQIFA
                     KAQCVVCHTIPGIPGATGTQGPKLEEATNAPQRIKDPAYKGTAHSTPEYIMESVVAPS
                     TYVVKGFPDNLMPKIFGQKLSAGALKKIVDYLSQVKAGAPPPKIS"
     gene            complement(5208..>5454)
                     /locus_tag="VFR82_07950"
     CDS             complement(5208..>5454)
                     /locus_tag="VFR82_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501569.1"
                     /translation="MGYIRSSVRLFWHVNEIMRDNSPWAYTHTVGFAANMISFNVLFF
                     WVSILFVFWLGSLTAKKVPVGEKVGVPGGAPQPAGSH"
BASE COUNT         1160 a         1600 c         1477 g         1217 t
ORIGIN      
        1 agcgcggcgc agcccgctcc ctgaaaggca atccactcat ctaccaggcg agtggcaccc
       61 agcaatgccg cagcctgtcc gtgtgcgcag aggacgagcc ggtggtgtgg atagcgtaag
      121 cggagcctgc ggatggccgg cacggcgagg aggaggtcac cgagcgatcc ggggtgcaag
      181 atgacgatcg tgcgtgccat gccgctcaag acgattcagg aaggatcttc tcatggagcg
      241 agcgggccgc cagaagatcg gcgggagtgt tgacgttata gaaggatcgc ccagccggat
      301 cgatcccaac caattcatcc gccgtcacca gacgcacgcg cagcgacgga tgatcggcca
      361 attgctgaat tttcaattcg tgcgcctgaa tgagactatc catgacgggc agacagcgtc
      421 gatgatagag ggcgtgcatc ggttgcaatc tattctcaag tttggccatg accagatccg
      481 cttcaccctg tacggtgaga aaataccgga cggtgacggg gtcaagaaag ggcatgtcac
      541 aggctacggc aaacaccgat ggcgtcagtg cttgcttcag acccgtatag agaccgccca
      601 agcttccgca gcccgggatg agatcacgcc agaccggcac ctcagattcg agaggaggac
      661 tgtcctgggc aatgacgacg acgaccgttt gaaagaccga acgcagaacg gtcagactat
      721 gcgcatagag tgtttcctct ccgatggaca aaaagcgctt atcctccccc attctttggc
      781 ttttgccgcc cgccaggagc acgcctgtca catcggcaat cataggttgt tcacgcttct
      841 gttgacaaaa aacaagaggg ggtgggtttc cccaccccct ccgtggtgtg tcacatcgag
      901 atcctgacgc aaactagagc gtcttcccct tcttcttatt cgcgcgtcgg gtcatgaccc
      961 atccttccag cagtaccacc ccgaaggcaa tgattgccgg atagatgtaa gtttccttcg
     1021 ggatcggggg attcaggaac gtgtagtaga aggccgatac ggccatcttc cgtcccgcat
     1081 cgccattgtg cccgtcccag gcgaagaaca cggtcggcac atattgcccg acttcgaaga
     1141 aggtatcttc gtcgtaatcc tgatccttct tgttgccgag cgggcgttga atcattacat
     1201 accagcggcc gttcttccat tcacccttga ggaccttcag gttctcctca taggtatcac
     1261 gctcctcgaa gtctttgtcc catcccgtgc ccttgaacgc ccggatggat ccgtcggctt
     1321 cccactttac gatgtccacc gggaactgct cgttggtccc gaacagatac cgcggcttga
     1381 tcggagcggg caattccttc catttcacag gtgtttcgat cgcgatggcg tcgttgtaca
     1441 ccttgtagtt gttttgattc gacgcgatgg atccttcctc gccggtcttc ggagcctgct
     1501 ccttgatatc caggtttact tgcgtgggag cccagggcag cttgccctcg gccacgctct
     1561 tggtgcggtc atcccactcc agaagataga cgataaattt gtcgttgtag agcgaacgaa
     1621 cccagatgtc gtcgatccgg ttcacgaagt tgcgtggctt gtgcgtgatc tgtccaccca
     1681 tggccacgta ccgtttggat gccttctgcc acgcttcatt ctccatatcc gtcggaattt
     1741 cgccctcgac cgggtcggac ggcaccacga agttgatctt cggcttatcc gtcaacggat
     1801 cgatggggag aggctttcca tccgtatctc gctcgcacag aaaattgacc caattcgcaa
     1861 tgtgccaacg ttcctcaacc gtggtgttgt ccgcgaatga gggcatcgga gttccgttca
     1921 caccagtcga gaatgtccgg aaaatgtttc gcacgttata ggggtcttga cggctgccac
     1981 gaaagttcca gcacttgtgc cagtcggccg gctgaatggc aaaaccccag tcgtctttca
     2041 ggttgaaggc gtttccgtca ccccggcctt cagcaccatg acactcaacg cactttttct
     2101 cgacaatgag ctccgatccc ttcttcaggc tttcctccgt cgcagcgatc ggcttgatat
     2161 cgcctagctg gagaacagtc tgggcctcgg actgcttgtc cgtaaacttc cggtccttga
     2221 ccaactgggt tgtcacgaat gagagcactt gcaacctttg ctcttccgtc agaataccct
     2281 cccacgaagg catggcggag cccggtaaac catgcgtgac ggtctcgaac aggtcgttct
     2341 gtcccggaac tggcttccgg gaatcgaaca agggaagctc tccactcgcg gtatgccgga
     2401 ttttgaacgt gccttgattg aagttgcgcg ggcggggcca gagacgatcg gcgccaggcc
     2461 catcgcctgc gccctcgacg ccgtgacacc acacacactt ggtaaagtag acgcgcttgc
     2521 ccgcctcgat catctcggca gaaggctccg gggccagttc gcccttcttg aacccttcgg
     2581 gcatgtcttc ggctccgatc agcggaacgc tgacgcttcc agacgccagg atgattccga
     2641 aggcggcgac tgcgaccact cctgccttcc cacttgaact gttcatcatg tttaccctca
     2701 tctcaatatg tagatccagg ttatgaatcg ccttagacga ttagtcccag gtacgtgggt
     2761 agtagcccgt gtgccaatat tcgaacagga tcaccttcca gatttcatcg actgtcaggt
     2821 gctgttccca aggcggcatg acagacgccc atggaaagcc ttcgttcggc aatccgatgc
     2881 cgcccttggc cacgcgccag aagatgaatg tctcttgcaa ctgggcgatg gttcccggat
     2941 cggtgaaatt ggccgggatg ggattaaagg caaaggcgtg caacccacgg ccgttcaagt
     3001 tatcgccgtg gcagaaatgg cagttctgga agaaaatctc cccgccctca cggacatact
     3061 tcagatatcc cgtgctcttt tcatcccagg ggttggcgtt gggcttcatc aaccggccca
     3121 taccctgttc cacgatgttg gcatttgtaa attcctgatc gtactttccc tcgggattga
     3181 cgcgatacgg attctgtgac gtctgaagca cataagtctt gccatgcacc ttcgtgctgg
     3241 ccggcggcgc cggatgtacg gtacgcaact caatcggctc ctcagacttc ggtttcatcg
     3301 cgttgtacga gaatccgccg atgagaatcg ggagcaggat gagataggca taccgcagca
     3361 ccttgttgcc gccgctctgc gcatcaagga cgttcaaaat cggctgcttg aatttcttcc
     3421 agtcttcctc gttcgaggac acccacatga agacggcgac aagggacacc gtgccataca
     3481 tggcacgcac gctgaacggg atcggaggat aaacccggta cttcaggtac agcagaatga
     3541 acgcccagaa tccgataccc tgccagaacc tcgggagcgg catccccatc gaactcatca
     3601 tgtaactgag cccggcaaag cccgccacga agagcgttaa ctcaaatagc atctgcatcg
     3661 gcatatagcc ttcgaccaag cgcacggtat tggagggaac gacatcgaag atcatcccga
     3721 ccagcaggag aagtccgacg actcccacca aggcgcccac tgacatcaat gctttcatgg
     3781 gtaaactccc ttatgtctac agatagacgg ccaagattat gaaatcttcg gtggcggtgc
     3841 gcccgccttc acctgcgaca aatagtcgac aatctttttc aacgctccgg cgctcagctt
     3901 ttgtccgaaa atcttcggca tcaggttgtc ggggaatccc ttcaccacat aggtgctggg
     3961 agcgaccacc gattccatga tgtactcggg agtgctatgt gccgttccct tgtacgccgg
     4021 atccttgatc cgctgaggtg cgttcgtggc ctcttccagt ttcggaccct gcgtgccggt
     4081 cgcgcccggg atgcccggaa tcgtgtggca gacgacgcat tgcgccttgg caaaaatctg
     4141 gtcaaccggc tccgacccgt cggccagtaa cgcgctggct gcccccgcag gcttgtcttc
     4201 ttgctgcttg ggacgctcgg cttccggaat gaatttttcg taggacttcg tgatctcctc
     4261 gaatgccggc gcttcccgtc cttcgcggac atacaaccac gtatcgaccg ccgccaactc
     4321 aggtagcgac agcgatatcg gtggcttgtg aatcttgggc atggggcttt ctttatcgtt
     4381 agtgcccttc acaccgtaac ccgacactac gtagcaactg ggacaggaat gtgactcggc
     4441 gatgtattcc tggccgtttt cggcggtacc gcagcctgga cacgattcct tttcggcgta
     4501 ctcgcgtttg gccgggttac cctttgaata cttcggatct tcgagccgtt ccttgcctgc
     4561 ccgctctggg agaccgagca agttgggagc ccgttcgccg agcatgccgg catggaaggc
     4621 atggcaaagc gggcactgcc ctttaccgat tgccccttgt actttatttt gcccgatgcc
     4681 accgaaaatg atcttttcac cttcatctgc gagctgctgc gggctcatcg aattgaagtc
     4741 gagcttttcc tctttgggag gaaagccgcc ctcgacttga gggagccaat tcccgtaacc
     4801 ggagagaaag gcggcaacga agaacatgaa cccgccgatc ttcagcagcg ccgcgagatt
     4861 cgtaaaatat atcgccatga cgaaggtcgt gacggtaatc atcacgagag agaccggcaa
     4921 caaccggttt tctccataga agttattctt gtagttggcg acggcgatga acatcaagat
     4981 cgccgcgatg ataccaatga aggttttgaa ggtcgcccgt ttcttggcgg caggatcgct
     5041 gatggagact tgaaagtaga tgagtaaacc cgctagcagg gccaggacca cccagcccat
     5101 tgagagagct tcattgatca gattacccac ggtttaaacc tcctgcggct acaccggtgg
     5161 gagacgcgag catgtctccc accgcgagcc aaactcaata gggatggtta gtggctgcca
     5221 gcgggttgcg gcgcgccgcc aggaactccc actttttcgc cgaccggcac tttcttggcc
     5281 gtcaaactcc cgagccagaa cacgaagagg atgctgaccc agaagaacag cacgttaaac
     5341 gaaatcatgt tcgcggcgaa tcccaccgtg tgggtgtagg cccagggtga gttgtcccgc
     5401 atgatctcgt tgacgtgcca gaacagcctt accgaggagc ggatatagcc catc
//
LOCUS       DASTQY010000241        10011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1157616, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000241 DASTQY010000000
VERSION     DASTQY010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10011
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157616"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFR82_07955"
     CDS             <1..288
                     /locus_tag="VFR82_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HEU4501570.1"
                     /translation="FFGAQFYLSQRNYGESPLYSFLFSTFTSTCFEYFIESWSERPSI
                     TDLIVTPIVGSILGEVVYQATRHMRKDGFTTAEKIIITVINPLYVMQNGYH"
     gene            complement(445..1275)
                     /gene="smbP"
                     /locus_tag="VFR82_07960"
     CDS             complement(445..1275)
                     /gene="smbP"
                     /locus_tag="VFR82_07960"
                     /inference="COORDINATES: protein motif:HMM:NF028095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HEU4501571.1"
                     /translation="MKHKRVVLSCAGSLLLVFASATHAADTPVETTPTDAAAAPQTEA
                     AVNSEATITPQTDAAVNSESAVTPQAEERMSVAAETSPDTRIDPAAERMATSSGPCAD
                     QSGSASTSMDCERLKRRLGENRTPGNLTAACSSRMDDKGNVMYDDPTCPGRIHPVVTD
                     ARSVYLTRENPFFNLHIEKAVDHARAAEIAGNQGQAIDLLEHAQMSLDRAKEAQRAGN
                     VPGLNEAIMSLREALRLPEASSVREATAYVRDARKNLSQAGGMKYAEIHPKGVVAVSK
                     "
     gene            complement(1571..2614)
                     /locus_tag="VFR82_07965"
     CDS             complement(1571..2614)
                     /locus_tag="VFR82_07965"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEU4501572.1"
                     /translation="MNRISTLILTIVASGVVVLLGGLFTLWLLALPSKGEALAHDAGL
                     AHPTLLAHRGASYLAPEETRPAYLMARELGVDYLELDLQRTRDGILVAIHDDILSRTS
                     NIDEVFPERKGDSVDTFTFAELQQLDIGDWFNRAFPERARKSFHGLRVLRLQEILEIA
                     EATSPRIGLCIETKRAHRFSGIERQLVEELRGREWIQHQGAPGRASLIFQSFDPDSLE
                     RLKVLAPEISRLLLVDEALLKRLAWEGVLRVAERLATGIGPWGYGWAFGPEWSSGVGR
                     RYVPTWPWYTRAAHQAGLIVFPWTIDDRWEMWMVRLAGADGIFTNRGELAMEVFGRSP
                     RPDIEALWQKIGY"
     gene            complement(2731..3579)
                     /locus_tag="VFR82_07970"
     CDS             complement(2731..3579)
                     /locus_tag="VFR82_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501573.1"
                     /translation="MRSSIYSSMMVFMVLASAPAWADDLQHAAALPGDHAPVVFVAHD
                     YGFTGPDQLPSGLTTVRIVNEGQDLHHVQFLRLLQGKSAADFRAAIAENPNRLPGWVQ
                     YAGGPNAHLPGSRASATVNLTEGDYVLMCWIPDKNGVPHVALGMQKALFVRGGKPTRV
                     STPKPSLTVKQVDYQFVLSKRIESGFRTIEVMNHGTQVHELVVVKLASGATIKDVIAS
                     FEPGAAGPPPGELVGGITGIEKGERVQFTTEFQPGRYGLICFVPDAITGQPHFLHGMT
                     TEFTVE"
     gene            3717..4178
                     /locus_tag="VFR82_07975"
     CDS             3717..4178
                     /locus_tag="VFR82_07975"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU4501574.1"
                     /translation="MIPDSILNLLQTGVSVMVGTRDASLMPECTRAWGIHVGADRGTV
                     IVLLSEAFAGQTLDNLRENGRIAVTCTRPTDHITCQLKGRVRSMKPVTSANRETSRQW
                     HREFIAELRAIGVPSALGEAWIVEPTVAVEMAVTDVFDQTPGPGAGKKISR"
     gene            4175..5557
                     /locus_tag="VFR82_07980"
     CDS             4175..5557
                     /locus_tag="VFR82_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU4501575.1"
                     /translation="MKKTCLQLEDLWACFQGTVPAALSTCAKDGTPNITFISQVYYVD
                     PKHVAISFQFFNKTHSNVRENPFACVVLLDPRTLHAYRFRLRFDHSESTGALFESMSL
                     QLQAVASYSGMADVFRLLAADVYEVLGVERLEGFTRLPAPPPSERSSRLFVDSGLVSL
                     RLIAERLNKTELLDEVLQVSLQLLDEQYGFQYSMILLMDERNAKLLTIASHGYQENGA
                     GSEVGMGEGLIGMVARARRTLYLSAMDHSLRYARAVRDRAEAIGQAEAVAREIPLPGL
                     PDAASQIAVPLVTRGRLVGVLAVESKDPLAFLPRENTLLTAIGSHLATAIDQLSRETD
                     DVPVERPRRAPVPASRPASTRRFTFFQKDDCICVDGGYLIRNVPARILWRLLRQYRDD
                     GRVEFTNRELRMDTWLGLPEIKDNLETRLILLRRRLEQKCPDVRLVQRGRGRFALEVD
                     VAIDLTEKES"
     gene            complement(5597..6157)
                     /locus_tag="VFR82_07985"
     CDS             complement(5597..6157)
                     /locus_tag="VFR82_07985"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU4501576.1"
                     /translation="MKEDQARSACAVFVGVSLDGYLARPNGDLDWLMGEGGGDSAEYG
                     YNEFIAGIDAIVMGRKTFEKVVMFEKWYYGNKRVVVLSHHPLDLSVARARGGVVEQLE
                     GSPAEIVSKLAASGACRLYVDGGLTIQEFLRAGLIRRLIISRLPVLIGEGIPLFGSLP
                     HDIHLNHIATRTYPGGMVQTEYSIAS"
     gene            complement(6218..6664)
                     /locus_tag="VFR82_07990"
     CDS             complement(6218..6664)
                     /locus_tag="VFR82_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU4501577.1"
                     /translation="MGTNTIRLHRVFRTAPERIYRAFLEADAMVKWLPPNGFTGKVHH
                     MDAKVGGTFKMSFSNFTTGKSHSFGGQYLELVPHERLRYTDNFDDPNLPGEIRVTVAL
                     KKVSCGTEVNIVQEGVPDVIPAEACYLGWQESLILLGKLVEAEIPD"
     gene            complement(6776..7312)
                     /locus_tag="VFR82_07995"
     CDS             complement(6776..7312)
                     /locus_tag="VFR82_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501578.1"
                     /translation="MNPRIKVWAALLLALWLLPAVIEAQTPRQQAEKELERKAPNAGV
                     EACSLITRADVKTATGSDPFVDPEPAGQGGWICNVGTGELKVYSGPKSWEAWESTLKS
                     FKLDKEPRTPASGFGEQAYFVYLKPANKHQSNVAVLVAKSGSHTLALSLDASDGNPAE
                     SMRPALESLMKTILSRLP"
     gene            complement(7440..7817)
                     /locus_tag="VFR82_08000"
     CDS             complement(7440..7817)
                     /locus_tag="VFR82_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU4501579.1"
                     /translation="MKYMLLCCHEEKKVDSMSKSDVDALMEETMAYCEALKKSGHLLA
                     VEALEPIQAAMSVTVRNGKLAVTDGPFAETKEQLGGFFLISARDLNEAIQVASKFPSV
                     RFGTMEVRPVRDLPSSGRLAAAS"
     gene            7994..8701
                     /locus_tag="VFR82_08005"
     CDS             7994..8701
                     /locus_tag="VFR82_08005"
                     /inference="COORDINATES: protein motif:HMM:NF021385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEU4501580.1"
                     /translation="MTIQDRLAELDWKAIEASLWQRGYAKTDALLTVEECDALVALYS
                     MDQLFRSRIDMKRFRFGEGEYKYFTYPLPPLVQTLRENIYPRLAVIANAWAKSLGQPE
                     MFPLSHDKLLAFCRLKGQTKPTPLLLRYAAGDYNCLHQDLYGAVAFPLQLTAFLSRPD
                     RDFSGGEFLLVEQRPRAQSRGEVLAPEQGEIVIFATRHRPVQGSRGYYRVNLRHGVST
                     VRSGSRFTLGVIFHDAK"
     gene            8742..9623
                     /locus_tag="VFR82_08010"
     CDS             8742..9623
                     /locus_tag="VFR82_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU4501581.1"
                     /translation="MRIATWNVNSLKARLEKVMWWLDRAKPDVLLMQETKLVDADAPV
                     KTFNEAGYELVHHGEGRWNGVAVASRCGIGDVITNFGEPLRPPRTSETGDDEPLAEAR
                     MLAAECAGLRIISVYAPNGRLVGSPFYEAKLVWFDKLARWINKAVTRETPAVIGGDFN
                     VAPEDIDVWDPRACHGGTHVSDRERTAFARLSSAGFVDAYRLHHPEPGRYTWWDYRAG
                     NFYKNFGMRIDHLLVTTSLKHRVVWAEIDREARKGKPVPSDHAPLVIDIDKPGHPFDA
                     GWASAESRIASRRHEQR"
     gene            9669..>10011
                     /locus_tag="VFR82_08015"
     CDS             9669..>10011
                     /locus_tag="VFR82_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501582.1"
                     /translation="MLARIVLVLASAALLSCGGGDKHFPSSNPPEYDPTKSHAPPVTP
                     PTSKPVVQDSKPAEEPALAVLPLEPAPNEVGQWRKLESEKLYPRKRAESSCEALTQLL
                     QGLGSTHLLNGR"
BASE COUNT         2121 a         2968 c         2756 g         2166 t
ORIGIN      
        1 ttctttggag cgcaattcta tctatcccag aggaactatg gtgagagtcc cttgtatagc
       61 tttctgttct caaccttcac atccacctgt ttcgaatatt tcatcgaaag ctggtccgag
      121 cgcccaagca ttaccgacct catcgtcaca cccattgtcg gctcgatcct tggcgaagtg
      181 gtctaccagg ctactcgaca catgcgcaag gacggcttca ccaccgctga aaagattatc
      241 atcacggtga ttaaccccct ctatgttatg cagaacggct atcactgaca gaaccggttt
      301 tataagacgg gaccgacgag gcagttctcg aatacgattt cagcagagtc gagggatcgg
      361 ttgagatgcc cactgcaatc acagggcaat tcacaattga ggggtgagca aggcagcgcc
      421 tcactcaccc tctcgacgat tggatcattt agaaaccgcg acgactccct tggggtggat
      481 ttccgcatat ttcatcccac ccgcctgtga caaattcttt cgcgcatctc gaacataggc
      541 ggtcgcttcc cgcacagaag aggcttcggg cagcctcaag gcttctctca aagacatgat
      601 ggcttcattc agcccaggca cattcccggc ccgctgcgcc tctttggctc gatccagcga
      661 catctgagcg tgttcaagta ggtcgatggc ttgcccctgg ttccctgcga tctcggcagc
      721 acgggcatgg tctactgcct tctcaatgtg gaggttgaag aatgggtttt ccctggtcaa
      781 gtacacgctt cgagcatcgg tcacgaccgg atgaatccga ccagggcacg tcggatcgtc
      841 atacatcaca ttgcccttat catccattcg ggagctgcag gccgccgtca aattcccggg
      901 cgtccgattc tccccaagtc gacgtttcaa tctttcacaa tccatggatg tcgaggcact
      961 tcccgattga tcggcacaag gcccggaact ggtcgccatc cgctcggccg ctggatcgat
     1021 cctcgtatcg ggagatgttt ccgccgcaac agacatgcgt tcctcagctt ggggcgtaac
     1081 ggctgactcg gaattgacgg ccgcgtcggt ttgaggagtt atggtggcct cagaattgac
     1141 ggctgcctcg gtttgaggag cggcggcggc atccgtgggc gttgtctcaa cgggcgtgtc
     1201 ggccgcatgg gtggctgagg caaataccag gagaagactt cccgcacaac tgagcacgac
     1261 acgtttatgt ttcatggccg cctcctttcg gtttactcat cacgaggttt tccttacgga
     1321 agtgccagtc tgttgtaatt cgatcaaagt caatactggg catacccctg gcggccggta
     1381 attttactaa gctgaaacga tcagtctggg tgccttgtcg ctggctggtt ttgggtgaca
     1441 gtccaacgcc ccgtctcgcc ggttcgttcc gagtcaaggc ccattacgat aaactgaccc
     1501 ctaatacgga tcgatgcctg cccacctcca cgccggcgct tcgtggggcc agggcccatt
     1561 catctctcgg tcagtacccg attttctgcc acaaggcttc gatatccggc ctcggcgagc
     1621 ggccaaagac ctccatggca agctcgccgc gattcgtaaa gattccatcg gctcctgcca
     1681 gtctcaccat ccacatttcc cagcggtcat caatcgtcca gggaaagacg atgagtcccg
     1741 cctgatgtgc cgccctcgta taccagggcc aggtgggcac atagcgtctc ccaaccccgc
     1801 ttgaccattc cggtccaaat gcccagccgt acccccacgg tccgatacca gtggcaagtc
     1861 tctcagcgac tctcagcact ccttcccacg caagtcgttt cagcagtgcc tcgtccacga
     1921 gaaggagccg ggagatttcc ggcgccaaca ctttcaagcg ttccagactg tcggggtcaa
     1981 acgactgaaa gatcagagac gcgcgacctg gtgcgccctg atgctgaatc cattcacgtc
     2041 ctctcagctc ctcgacaagt tggcgttcga tacctgaaaa ccgatgcgct cgttttgttt
     2101 caatgcaaag accgattcga ggggaggtcg cctcggcaat ctccaagatt tcttggagcc
     2161 gcaggactcg gaggccatgg aaactcttgc gagcccgctc ggggaatgcc cgattaaacc
     2221 aatcaccgat atccaactgc tggagttcgg caaacgtgaa cgtatcgacg ctatcgcctt
     2281 tacgttcagg aaagacctca tcaatattcg atgtccgaga gaggatatca tcgtgtatcg
     2341 cgaccaatat gccgtcccga gtacgttgta ggtccagttc caggtagtcc actccgagtt
     2401 ctcgcgccat caagtacgcg ggtcgagtct cttcaggcgc cagataggag gcgcctcgat
     2461 gagcaagaag cgtcggatgc gccagacccg cgtcatgtgc caaggcctcg cctttggacg
     2521 gcaaggccag aagccacaat gtgaataacc cgccgagcaa gaccacgaca ccgctcgcca
     2581 cgatggtgag gatcaatgtc gatatccggt tcatgacagg gacttctcca gtctgatggt
     2641 gtagcgggtt ataaagaaaa agccggtcag gttctagacc ctgaccggct ttttcctctc
     2701 cagccgcgcc cgcgtgcttt gttacagccc ttactccaca gtgaattcgg tcgtcattcc
     2761 gtgcaggaag tgcggttgcc cggtaatcgc gtccgggaca aagcaaatca gtccatatcg
     2821 gccaggctga aattcggtgg taaattgcac gcgctcccct ttctcgattc cagtaatccc
     2881 tcccaccaat tctccgggag gaggacccgc cgcgcctggc tcgaacgagg cgatgacatc
     2941 tttgatggtc gcgcccgatg ccagcttcac gacaacaagc tcatggactt gggtgccatg
     3001 gttcatcact tcgatggtgc gaaacccgga ttctattcgc tttgagagaa cgaactgata
     3061 atcaacttgc ttcacggtga gcgacggttt gggcgtggag accctcgtcg gctttccgcc
     3121 tcggacgaac agcgccttct gcatccccag cgcaacgtgc ggtacgccat tcttatcggg
     3181 aatccagcac atcaacacat agtctccttc ggtcagattt accgtggcag acgcgcgact
     3241 gcccggcaga tgggcattcg gtcccccagc atattgcacc catcccggca gccgatttgg
     3301 gttctcagca atggcggcac ggaagtccgc cgccgatttt ccctgcaaca acctcaaaaa
     3361 ctggacatgg tgaagatcct gcccttcgtt cacaatcctc acggtcgtga gaccggaggg
     3421 gagctggtct ggaccggtga agccgtaatc atgggccaca aacactaccg gtgcatggtc
     3481 gccgggcagt gcagcggcat gctggagatc atccgcccag gccggcgcag acgccaggac
     3541 cataaacacc atcatcgaac tgtatattga gcttcgcatt ggaccctccc tctgttaacg
     3601 cgctccacgt gaacgcggtt gtgtcatgag ttgcgagggt gacagtaaca cggaggtccg
     3661 cgacatggtt cctaaaacta tgctaaggtt tccctaaatt tttcctaaca gaccacatga
     3721 ttcccgactc gatcctcaat ctcctccaga caggtgtctc ggtcatggtc ggtacgcggg
     3781 atgcgtccct catgcccgaa tgcacgcgcg catggggcat ccacgttggt gcggatcgtg
     3841 gcacagtcat cgtccttttg tccgaagcct tcgccggcca gacactcgat aacctgcgtg
     3901 agaacggtag gatcgcggtc acctgcactc gaccgacgga ccacatcacc tgccagctca
     3961 aaggccgagt ccggagcatg aaaccagtca cctctgccaa tcgagagaca agccggcagt
     4021 ggcaccgtga gttcatcgcg gaattgagag ccattggcgt gccctcggca ttgggtgaag
     4081 cctggatcgt cgaaccaacc gtggccgtgg aaatggcggt caccgatgtc ttcgatcaaa
     4141 ccccggggcc tggtgcgggg aaaaagatca gtcgatgaaa aagacgtgcc tgcaactgga
     4201 ggatttatgg gcatgctttc aaggcaccgt tcccgccgcc ttgagtacct gtgcgaaaga
     4261 tggcactcca aacatcacct ttatcagtca ggtctattat gtggacccga agcacgtggc
     4321 gatttcgttt caattcttca ataagacgca tagtaacgtg cgcgaaaatc cgttcgcatg
     4381 cgtcgtcctc ctcgacccgc gaacattgca cgcctatcgc tttcgtctcc gcttcgatca
     4441 ttccgaatca acgggagcgc tgttcgaatc gatgtcgctt caactccagg ccgtggcatc
     4501 gtactcggga atggccgacg tttttcgact gctcgccgcc gacgtctatg aggtgctcgg
     4561 cgtcgaacgc ttggagggtt tcacccggct gcctgctcca ccaccatccg aacgctcttc
     4621 acggcttttt gtcgactcgg gactcgtctc cctgaggctc atcgccgaac gcctcaacaa
     4681 gacagagctg ctcgatgagg tccttcaagt ctcgctccag cttcttgatg agcaatacgg
     4741 cttccagtat tcgatgattc tgctcatgga tgagcggaat gcaaagttgc tgaccattgc
     4801 cagccacggg taccaggaga acggagccgg ctcagaagta ggaatgggcg aagggctgat
     4861 cggcatggtc gcgcgtgcgc ggcgaaccct ctatttgtcg gccatggatc acagtttacg
     4921 atacgcgcga gccgtgcgag accgcgccga agccatagga caagcggaag ccgtcgcccg
     4981 tgagattccg ctcccgggtc tgccggacgc agccagccag attgccgtgc cgctcgtgac
     5041 tcgtggccgg ttggtgggtg tcctggctgt cgaaagcaag gatccgctcg cttttctccc
     5101 ccgcgaaaac acgctgctga ccgcaatagg ctcgcatctc gcaaccgcga tcgaccaact
     5161 cagccgcgag acggacgatg tgcctgtgga gcggcccaga cgtgccccgg tacctgcgtc
     5221 tcggccggcg tctacacgac gcttcacctt ttttcagaag gacgactgca tctgcgtaga
     5281 tggcggctat ctcattcgga atgtgccggc gaggatcctc tggcggttgc tgaggcagta
     5341 tcgagatgac ggccgagtgg agttcaccaa tcgtgaactg cggatggaca cctggctcgg
     5401 actacccgag ataaaggata acctcgagac tcggctgatt ctgcttcgtc gacggcttga
     5461 gcagaaatgc ccggacgtac gattggtcca gagaggacgc ggccgcttcg cattagaagt
     5521 cgatgtcgcg atcgacttaa cggaaaaaga atcgtaaacc cctcgatgcg ctcccctact
     5581 tcctcgaagg cgggctttat gaagcgatag agtactcagt ctgcaccatg ccgccaggat
     5641 aggtacgtgt ggcgatatga ttgagatgga tatcgtgcgg gagggagccg aagagtggaa
     5701 tgccttctcc gataagcacg ggtagacggc tgatgatgag acggcgtatg agtcctgccc
     5761 gtaggaattc ttgaatggtg aggccaccgt caacatacag acggcacgca ccggacgcag
     5821 ccagcttgga cacaatctcc gccggggatc cctccaactg ctcaacgaca cctccgcgcg
     5881 cacgcgccac ggatagatca agcggatgat ggctcaagac gaccactcgc ttgttgccgt
     5941 aataccactt ctcgaacatc acaactttct cgaacgtttt tctccccatg acgatcgcat
     6001 caataccggc aatgaattcg ttatagccgt actctgcgct gtcaccgcca ccctcaccca
     6061 tgagccagtc gagatcgccg ttcggccgcg cgaggtatcc atcgaggctc acgccgacaa
     6121 agaccgcaca tgcggatcta gcctggtctt ctttcatgcc cccaccctta cactctacta
     6181 gaacagcgag atcacgccca cgacgatctt tagaatccta atccggaatc tccgcctcga
     6241 cgagtttccc aagaaggatg agcgattcct gccagccgag atagcacgct tcggctggaa
     6301 tcacgtcagg cacgccttcc tgcacaatat tgacctccgt tccacaagag accttcttca
     6361 aagcgaccgt cacacgaatt tctccaggca ggttcggatc gtcgaagttg tccgtgtatc
     6421 gaaggcgttc gtgaggaacg agttccagat actggccgcc aaacgagtgg ctcttgcccg
     6481 tcgtaaagtt cgaaaatgac atcttgaacg tgccgccgac cttggcatcc atgtgatgaa
     6541 ccttgcctgt aaatccattt ggcggcagcc acttcaccat ggcatcggcc tccagaaacg
     6601 ctcggtaaat ccgttctggt gctgtgcgga atacgcgatg gagtcgaatg gtattggttc
     6661 ccacgattgt ttctcctttc tctagtggaa gcataacccg attgggcatc gcttgttgct
     6721 ttgtagtcga acgggctcgc acaaatcgac aggagcccaa tgggattcca ccttctcacg
     6781 gcaaccggga gaggatcgtc ttcataagcg attcgagcgc tgggcgcatg gactcggccg
     6841 gattgccatc ggacgcgtcg agtgatagcg cgagcgtatg gctgcccgac ttcgcaacca
     6901 agaccgcgac gttgctctgg tgcttgttag ccggctttag gtaaacaaag tacgcttgct
     6961 cgccgaagcc cgaagccggg gtccgcggct ccttgtctag cttgaaactc ttcagtgtgg
     7021 actcccatgc ctcccacgat ttggggcccg aatagacctt caattcgcct gtgcccacgt
     7081 tgcagatcca gccgccctgg ccggcgggct ccggatcgac aaatgggtcg ctgccggtgg
     7141 cagtcttgac gtcggcccgc gtaatcaacg agcaggcctc cacgcccgcg ttcggcgcct
     7201 ttcgctccag ttccttctcg gcctgttgac gtggcgtttg tgcctctatt actgcgggaa
     7261 gcagccacag cgcgagcaac aatgcggccc acactttgat cctggggttc atcgcatttc
     7321 cttaggtttc agattacggc tagcataagc agtgtccgcg tcaaggaact gtaacggcaa
     7381 actttagcga atgcatcctc ttttctatgg tctagattca gactcttcta tgtcaatcct
     7441 tatgaagcag ccgccaagcg gccgctgctg gggaggtccc tgaccggccg tacttccatg
     7501 gtgccgaacc gcaccgacgg aaacttagag gccacctgga tcgcctcgtt caggtcccgg
     7561 gcgctgatga gaaaaaaccc tccgagctgc tctttcgttt cagcaaaagg accgtcggtt
     7621 acggctaatt ttccgtttcg gactgtcaca ctcatggccg cctgaatggg ttcgagtgcc
     7681 tctaccgcaa ggagatgacc gctcttcttc agcgcctcgc aataggccat ggtctcttcc
     7741 ataagggcgt caacgtcgct cttggacatt gaatccacct tcttctcttc atggcaacac
     7801 agcagcatat atttcatggc ttagaccctc caatttgact gtgaaacgct gttcttgaca
     7861 gatagtcgtt cgcgatgacc acaatcgaca tcgagtctag gcacaattca gtccattatt
     7921 taggacgaat tggctgaagt ggtcaacgag cctccaaacg aattgctcct ctgggacggg
     7981 aagcgaggac tgaatgacca tccaagacag actggccgag ctagactgga aagcgatcga
     8041 agcatcgcta tggcaacgcg gttatgccaa aactgatgcg ttgcttacgg tcgaagagtg
     8101 tgacgctctc gttgccctgt acagcatgga ccaattgttc cgaagccgga tcgacatgaa
     8161 acgctttcgg tttggtgagg gggagtacaa atacttcaca tatcccctgc caccgctggt
     8221 ccaaaccctt cgcgaaaaca tctatccccg tcttgcggtg atcgccaacg cctgggcaaa
     8281 gtcactgggt cagccggaaa tgtttccgct gagccacgat aagctgctgg cattctgtcg
     8341 tctgaaggga cagacgaagc ccacaccgtt gttacttcgt tacgctgctg gggattacaa
     8401 ctgccttcat caggatctct atggagcggt cgcgttcccg ctgcagctca ccgccttcct
     8461 cagtcggccc gaccgcgatt tttccggtgg tgagtttctg ctcgtagaac aacgaccgcg
     8521 ggcacaatca cgcggtgagg tcttggcccc ggagcagggg gaaatcgtca tctttgcgac
     8581 gcggcaccgt cccgtacaag gaagtcgcgg ctattaccga gtcaacctgc ggcacggcgt
     8641 gagcacggtt cgctccggtt cacgctttac tctcggcgtc atcttccatg atgccaagtg
     8701 atgaccgtca agagacgcgg cccagatagg agaatcgttg catgcggatt gccacgtgga
     8761 acgtcaactc gctgaaggct cgcctggaaa aagtcatgtg gtggttggac cgggcgaagc
     8821 cggacgtact tctgatgcag gaaacgaagt tggtggacgc ggatgcacct gtcaagacgt
     8881 tcaatgaggc cggctatgag ctggtgcacc atggcgaggg ccgatggaac ggcgttgcgg
     8941 tcgcgagtcg gtgcggcatt ggcgacgtga tcacgaattt tggagagcca ttacggccac
     9001 cgcggacgtc tgaaaccggt gacgatgaac cgctggccga ggcgcggatg ctcgctgccg
     9061 aatgcgccgg actgcgcatc atttcagtat acgcgccgaa cggccggctc gtgggctcgc
     9121 cgttttatga agcgaagctg gtgtggtttg acaagctggc ccgctggatc aacaaggccg
     9181 tcacgcggga gacacccgcc gtcatcggcg gtgatttcaa tgttgcgccc gaagatatcg
     9241 atgtgtggga cccgcgcgcc tgtcatggcg ggacacacgt ttcggatcgc gagcggacgg
     9301 cgtttgcgcg gctttcgagc gcgggattcg tcgatgcgta ccgattgcat cacccggaac
     9361 cagggcggta cacgtggtgg gactaccgcg ccggcaattt ttataagaac ttcggcatgc
     9421 gcatcgacca cttattggtg accacctcct taaaacaccg cgttgtctgg gcagagatcg
     9481 atcgtgaggc gcggaaggga aaacctgtac cctcggatca cgcaccgctg gtcatcgaca
     9541 ttgataagcc cggccatccg ttcgatgccg gctgggcttc ggcggagtcg cgcattgcgt
     9601 cgcgccggca cgagcagcga tgaatccgtt gcaggttcac agaccgatcg gctagcatgg
     9661 cgctcgccat gttagcccga attgtgctgg ttctcgcgtc agccgccctt ctgagctgcg
     9721 gcgggggcga caagcatttt ccctcgtcca atcctccgga atacgacccg acaaagagcc
     9781 acgcgccgcc ggttacccca ccaacgtcca agccggtggt gcaggactcg aaaccggcag
     9841 aggaacccgc tcttgccgtg ctgccgcttg agccggcccc gaatgaggtg ggccaatggc
     9901 ggaagctcga atccgagaaa ctgtacccgc gcaagagggc ggaatcgagc tgtgaagcac
     9961 tgacgcagct cctgcaggga cttggcagca cgcacctact gaatggacgt g
//
LOCUS       DASTQY010000242         7610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_479033, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000242 DASTQY010000000
VERSION     DASTQY010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479033"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2854
                     /gene="gcvP"
                     /locus_tag="VFR82_08020"
     CDS             <1..2854
                     /gene="gcvP"
                     /locus_tag="VFR82_08020"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEU4501583.1"
                     /translation="FVHRHLGPTEADVREMLALLGLQSLQALTDATVPRDIQLGRPLD
                     IPQHRGEQAVLQELRDLAQENRIYRSYLGMGYYDCITPPVIQRNILENPGWYTQYTPY
                     QAEIAQGRLEALVNFQTMVADLTGLPLANASLLDEATAAAEAMAMCLAIARGTRQEKK
                     EFFVSQDCHPQTIAVLQTRAEPLDVTVHVGRTTTINFDNPQLSGMLLQYPTTDGYVGD
                     YQALVQQAHKAGMLVVVATDLLALTLLRPPGEFGADIAVGSSQRFGVPMGFGGPHAAF
                     LAAKEEYKRQMPGRIAGVSKDRSGQVAYRLSLQTREQHIRREKATSNICTAQVLLAII
                     AGMYAVYHGPDGLRRIAERVHGLAAVLAAGLQQLGFDIGSQEFFDTIHVRLTKTQCEQ
                     ILVRADDQALNLRRHEDDSLGVSLDETTEEGDVRRLLEVFVGHQRLPFTIQELSQTVR
                     TAYPANLARTTPYLTHEVFNRYHAEHEILRYLYRLQARDLSLTHSMIPLGSCTMKLNA
                     TAEMLPVTWPEFARLHPFAPAEQSRGYQELFRQLEAHLAEITGFAAVSLQPNAGSQGE
                     YAGLMVIRAYHRSQGEFHRDVCLIPVSAHGTNPASAAMVGMTVVPVACDKQGNVDLTD
                     LEAKAAEHRVRLAALMLTYPSTHGVFETDVRRICQIVHTHGGQVYMDGANMNAQVGLC
                     RPGDIGADVCHLNLHKTFCIPHGGGGPGMGPIAVARHLATFLPGHPITGLGGTQSIGP
                     VSAAPYGSPSILTIPWVYIALMGRDGLMKATQVAILNANYMAKRLEKHYPVLYTGTGG
                     FVAHEFILDLRQFKDTAGIEAMDVAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKAE
                     LDRFCHAMILIRAEIQDIIDGRQPRTNNVLKNAPHTAQAVTASQWDRSYSRELAAYPA
                     PWVKDHKFWPHVGRIDEAYGDRNLICTCPPMEQYST"
     gene            2923..4242
                     /locus_tag="VFR82_08025"
     CDS             2923..4242
                     /locus_tag="VFR82_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU4501584.1"
                     /translation="MDVIDRRNFLVRSGLALGTSLLASELPFLSRSGALSAPPSRSLD
                     WQAVRDQFPLSRDLIHLAGFFLASHPTPVREAIERHRRGLDADPIGYWLAHEKEQEAA
                     VLRTAADYLGVDPTEIALTDSTTMGLGLLYGGLALQQDQEILTTTHDHYSTDVSLRLR
                     AERTGATVRRISLYEQLGTISRESLVEKLVGQVRDQTRIVAVTWVHSSTGLKLPIAEM
                     ARALEKINASRVEKDRIIFCVDGVHALGVEDFRLSDIGCDFFIAGTHKWMFGPRGTGF
                     VWGHERAWPFANAIIPTFHSDAYDMWMRVIPPRPLPKAIYMTPGGFHSFEHRWALDQA
                     FVFHQAIGKPRVTQRIHDLNDHLKQGLAKMRRVTLRTPLSRDLSAGIVCFEVAGLEPD
                     QVVRRLRERGIVASVTPYATQYARVAPGLLNSPAEIETVLKAIDELK"
     gene            4362..4586
                     /locus_tag="VFR82_08030"
     CDS             4362..4586
                     /locus_tag="VFR82_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501585.1"
                     /translation="MSQILNMSEHTTDEELHRLTTLLLFHLVERCGGQVQFKLEDAQR
                     VRTSLTTKMVQMQVGDEVRLRIIDRLPELK"
     gene            4732..5142
                     /gene="arfB"
                     /locus_tag="VFR82_08035"
     CDS             4732..5142
                     /gene="arfB"
                     /locus_tag="VFR82_08035"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEU4501586.1"
                     /translation="MLHISAHVTIPDSEIELHAMRAQGAGGQHVNKVSSAVHLRFDVG
                     ASSLPPFFKEELLKLRDSRISREGVITIKAQQFRSQEQNREDALERLRALIQSVAIPR
                     KKRRPTKPTRSSQHKRLESKKRQSRLKTLRQSIE"
     gene            complement(5420..6199)
                     /locus_tag="VFR82_08040"
     CDS             complement(5420..6199)
                     /locus_tag="VFR82_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEU4501587.1"
                     /translation="MLLSGKKGLIIGVANKHSIAWAIAQSASSQGAQLFFNYQNDRLK
                     ENVEELVATLPGSKAFPCDAADDGQIETLMVHVGKEAGALDFLVHSIAFAPREELTGQ
                     FVNTTRKGFATALDVSAYSLVALTRAAVPLMTQGGSVVTLTYLGAERVVPHYNVMGVA
                     KAALEATVRYLAHDLGPRNIRVNAISAGPIKTLAARGVSGISKMVDHHKEFAPLRRAT
                     EQGEVGDTALFLVSSLGRGITGEVIYVDGGYNILGSLASVE"
     gene            complement(6794..7327)
                     /locus_tag="VFR82_08045"
     CDS             complement(6794..7327)
                     /locus_tag="VFR82_08045"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HEU4501588.1"
                     /translation="MAKQRTGPRLRDHLRPDLHVIFVGINPGLRSAAVGHHFAGHSNR
                     FWKLLFDSGMVDEPLSFREDWRLPEWRLGLTNIIDRPSAGIDDLDPGEYRARASALER
                     KIRRYQPRTIALLGVTIFRILFPDITRSSRLALGPTPARLAGIPVFLLPNPSGRNAHY
                     PYHVMLDAFALLRRQAH"
BASE COUNT         1668 a         2154 c         2205 g         1583 t
ORIGIN      
        1 cttcgtgcat cgtcatctcg gccccacgga agccgatgtg cgagaaatgc tggccctgct
       61 gggtttgcaa tcactgcaag ccctcaccga cgcgacggtg cctcgagaca tccaactcgg
      121 tcgccccttg gacatcccgc agcatcgcgg tgagcaagcg gttctacaag agcttcgcga
      181 cctcgcccag gagaatcgga tctatcgctc ctacctgggg atgggctact acgattgcat
      241 tactcccccg gtcattcaac ggaacattct cgagaatccc ggctggtaca cgcaatacac
      301 accctatcag gcggagatcg cccagggacg actggaagcc ttggtcaatt ttcagacgat
      361 ggtcgctgac ttgaccggcc tgccccttgc caacgcctct ctgctcgacg aggcgactgc
      421 cgcggccgaa gccatggcca tgtgtctggc aatcgcccgg gggacgcgac aggagaagaa
      481 agaattcttc gtctcgcagg actgtcatcc gcaaactatc gccgtcttac agaccagggc
      541 ggaaccattg gacgtcaccg tccatgtggg acggacgaca acgatcaact ttgataatcc
      601 gcaattgagc ggaatgttgc tgcaatatcc gaccaccgat ggttatgtcg gcgactacca
      661 ggcgctcgtt caacaggctc acaaagcagg aatgctggtt gtcgtggcga cggacctctt
      721 agcccttaca ctgttgcgcc cgcccggtga atttggtgcg gacatcgcgg tgggatcgag
      781 ccagcggttt ggcgtcccga tgggattcgg cgggccgcat gcagcctttc tcgcggctaa
      841 agaagaatac aagcggcaga tgccggggcg catcgccggc gtttccaagg atcgcagtgg
      901 gcaggtggcc tatcggttat ccctccaaac ccgtgagcag cacatcagaa gggaaaaggc
      961 gacgagcaac atttgcaccg cgcaagtcct tcttgccatc atcgcgggga tgtacgccgt
     1021 ctatcatgga ccggacggcc tccggcggat tgccgaacgg gtccatggct tggcggctgt
     1081 acttgccgca ggcctgcaac aactcggttt tgacatcggg agtcaggaat tcttcgatac
     1141 catccacgtg cgtctgacga agacgcagtg tgaacaaatt ctcgtacggg ccgatgacca
     1201 ggccctcaat ctccgccgcc acgaagacga ctcactgggc gtctcactcg atgaaacgac
     1261 cgaggaaggt gatgttcgac gcctgctgga agtattcgtc ggacatcaac ggttgccatt
     1321 tacgattcag gaactgagtc aaaccgtccg gacggcgtac cccgcaaacc ttgcccggac
     1381 gactccctat cttacccacg aagtgttcaa tcggtatcat gccgaacatg agattctccg
     1441 ctatctctat cggcttcaag cacgcgacct ctccctgacg cactccatga ttccactggg
     1501 ctcttgcacg atgaagttga acgcgacggc ggaaatgctt ccggtgacct ggcctgaatt
     1561 tgcccggctg catccgttcg ctccggcgga acagagccga ggatatcagg agctgttccg
     1621 tcaactggaa gcccatttag ccgagattac cggattcgcg gccgtgtcgt tacagcccaa
     1681 cgccggctcg caaggcgagt atgcggggtt gatggtcatc cgggcgtatc atcggagtca
     1741 gggagaattt catcgcgatg tctgccttat tccggtgtcg gcgcacggta ccaatccagc
     1801 cagcgcggca atggtcggga tgacggtcgt gccggtggca tgtgacaaac aaggcaacgt
     1861 agatttgaca gacctcgaag caaaggcggc tgaacaccgc gtgcgcttgg ccgccctgat
     1921 gttgacctac ccatcgacgc acggggtatt cgaaaccgat gtcagacgca tctgtcagat
     1981 cgtccataca cacggtgggc aggtctatat ggacggtgca aatatgaatg cgcaggtggg
     2041 actctgccgt cccggcgaca tcggcgcaga cgtgtgccat ttgaatctgc acaagacatt
     2101 ctgcattccg catggaggcg gtgggccggg catggggccg atcgccgtcg ctcgtcatct
     2161 cgcgacgttc ctccccggtc atcccatcac aggcctcggc gggactcagt caatcggtcc
     2221 ggtctcagca gctccttatg gaagtccaag cattttgacg atcccgtggg tgtacatcgc
     2281 gctgatggga agggacggcc tgatgaaggc gacacaagtg gccattctga acgccaacta
     2341 catggccaag cggctggaaa agcattatcc ggtcctgtat accggaaccg gcggattcgt
     2401 cgcccacgaa ttcattctcg acctgcgcca attcaaagac accgcaggta ttgaagcgat
     2461 ggacgtggcc aagcggttga tggactatgg attccatgcc ccgacggtgt cgttcccggt
     2521 cgccggtact ctcatgatcg agccaacgga aagcgaatcc aaagccgagc tggaccggtt
     2581 ctgccatgcc atgatcctta tccgcgccga aattcaagac atcatagacg gacgtcagcc
     2641 tcgcaccaac aatgtgctca aaaacgctcc acacaccgct caggcggtga cggcctcgca
     2701 atgggatcgc tcttacagcc gggagctggc agcctatccc gctccatggg tgaaggacca
     2761 taagttctgg ccgcatgtcg ggcgcatcga cgaagcctat ggcgaccgca atttgatctg
     2821 tacctgtccg ccgatggaac agtactcgac ctaggttgct ggtcccttgt cctacgtcag
     2881 ggacggcagt agagtagaca tcacgtcaat ggaatcttgc tcatggacgt gatcgatcgt
     2941 cgaaatttcc tcgtccgctc cggtttggct ctcgggactt cgctcctcgc aagcgagttg
     3001 cctttcctct ctcgatcggg tgcgctctct gccccgccct ctcgatcact cgattggcag
     3061 gcggtgcgag atcaattccc gctttcccgt gacctcattc acctcgcagg gttttttctt
     3121 gcctcacatc ccacgccggt acgcgaggcc atcgagcgtc atcgccgcgg attggatgct
     3181 gatccgatcg ggtattggct ggctcacgaa aaagaacaag aggcggccgt gctccggaca
     3241 gcggcggact atcttggcgt cgatccaacc gagatcgccc tgaccgacag cacgactatg
     3301 ggactcggat tgctttatgg cggtttagcg cttcaacaag accaggaaat actcaccacc
     3361 acccacgatc attactcgac cgacgtgtcg ttgcggctga gagcagagcg caccggcgcc
     3421 acggtgcgcc ggatctccct ctatgaacag ctgggcacca tctcgcgaga gagtttggtg
     3481 gagaaacttg tggggcaggt acgcgatcaa accaggatcg tggcggtcac atgggtccat
     3541 tcgagcaccg gcctgaagct gcccatcgcc gagatggccc gggcgttgga gaaaatcaat
     3601 gcgtcccggg tcgaaaagga ccggatcatt ttttgcgtcg acggagtcca tgcactgggc
     3661 gtcgaagatt tccgtctcag cgacatcggg tgcgatttct ttatcgcagg gacacacaag
     3721 tggatgttcg gtcctcgagg aaccggattc gtgtgggggc atgaacgggc ctggccattc
     3781 gccaacgcca ttattccgac ctttcattcg gacgcctacg atatgtggat gagggtcatc
     3841 ccgccgcgac cgttgccgaa ggcaatctac atgacacccg gcggattcca ttcattcgag
     3901 caccggtggg cactggacca ggccttcgta tttcaccaag cgatcggcaa gccgcgtgtg
     3961 acccagcgga tccatgacct gaacgatcat ttaaagcaag ggcttgcgaa gatgcggcga
     4021 gtcaccctgc gtacgccgct gtctcgagat ctgtcagcag ggattgtgtg tttcgaagtg
     4081 gcgggactgg agcctgacca agtcgtccgt cgattgcgtg aacggggcat tgtcgccagc
     4141 gtcaccccat acgccacaca gtacgcgcgc gtggcgcccg gacttctcaa ttcgccggcg
     4201 gagatcgaga cggtcctgaa agcgatcgat gagttgaagt aagtcatgga aaaagctgac
     4261 atattagatc ggcatgcgaa ccccttcttt cggggcattg tagagcaccc attcattgag
     4321 cgataagggg cttgcacatc cgcagaagga aggctgatcg catgtctcaa atcctcaaca
     4381 tgagtgaaca cactaccgat gaggagttgc acaggttgac cacactgctg ctgtttcatt
     4441 tggtcgagcg ctgcggaggt caggtccagt tcaaactgga agacgcccaa cgtgtgcgca
     4501 ccagtctcac gaccaagatg gtgcaaatgc aggtcggtga tgaagtgagg cttcgcatca
     4561 tcgatcgcct gccagaactc aagtaatcgc ccaatctttt gtggctccat tgtatctggc
     4621 tctctgtgac ctgcctgcgg tgttgggatc atcctgactg acgcttgtcc gcttcatcgt
     4681 catgtgaaag aatgccgtcg aaggtcgcca ctccgcgaga cgagctggca tatgctgcac
     4741 atctccgcac acgtcaccat tccggactca gaaatcgagc tccatgcgat gcgggctcag
     4801 ggagctggcg ggcaacacgt caacaaggtt tcctcggctg tccacttgcg gttcgatgta
     4861 ggtgcctcgt ccttaccgcc cttctttaaa gaagagctgt tgaaactgcg ggacagccgt
     4921 atctcccgcg agggcgtgat taccatcaaa gcacagcaat tcagaagtca ggagcagaat
     4981 cgtgaggatg cgttggaacg gctcagggca ttaattcaaa gcgtcgcaat cccacgaaag
     5041 aaacgacgac ccacgaagcc gacgaggagt tcccagcata agaggcttga gagcaagaag
     5101 cggcagagtc gactgaagac gctgcgtcaa tcgattgaat aaccagctgt tcagtttgtg
     5161 ttcacccgcc ccaccccgaa gcatctagaa ggttcatcca cggtcggctg cagcgggcga
     5221 gaaggtggat gaccggctcg cttcaatcac atgcattcag agacgtccta tacctgagcg
     5281 tggtctttca tgattgatcc gttgaccggc aggtaaggtg gtgcgccccg gcaaactcga
     5341 tgacaaacca gtccagggtc gggcggatgt caggaatttc caggatacgg taggtcagaa
     5401 cgggatttct caatagccgt tattctaccg aggcgagaga tcccaggatg ttgtagcccc
     5461 cgtcgacgta aatcacttcc cccgttatgc ctcggcccag cgagctgaca agaaacagcg
     5521 ccgtgtcgcc gacttcccct tgttcggttg ctcggcgaag cggggcgaac tccttgtgat
     5581 gatcgaccat tttgctgatg ccggatacgc cacgggcggc gagcgttttg atcggtccag
     5641 ccgagatggc attgactcgg atgttgcggg gtccgagatc atgggccaaa taccggacgg
     5701 tggcctcgag cgcggccttg gcgacgccca tcacgttgta gtgaggcacc acccgttcgg
     5761 ccccaagata ggtcaaggtc acgactgagc cgccctgggt catcaagggg acggccgctc
     5821 tggtcagggc aactaaggaa taggcgctga cgtcgagcgc agtagcaaaa cccttgcgcg
     5881 tcgtgttgac aaactggccg gtcagctcct cccgtggcgc gaacgcgatt gagtggacca
     5941 gaaaatcaag cgccccggct tccttgccga cgtggaccat gagcgtttca atctgaccgt
     6001 cgtctgcggc gtcacaaggg aacgccttcg agcccggcag agtcgcaacc agttcttcca
     6061 cattttcttt gaggcggtcg ttttgataat tgaagaagag ctgcgcaccc tgactggacg
     6121 cggattgagc gatggcccag gcgatactgt gcttattggc cacgccgatg atcaatcctt
     6181 ttttgccgct cagtaacata ctgttctgct ccaggcggga tgcgagaata tattcctgtc
     6241 gcgtccgatg attactcagg gggaagatag catgattggc cgggactgtt ccaggcgaga
     6301 gaatggaggg cacagagccc gtgcgtgtca atctgtgcga tcaccagagt gctgttttgg
     6361 attggtatgg atttgacctg aggggagagg gagtttgaag gtttctcggt tgtgcgacgt
     6421 gtcgaacagc tgatgcacga cccggtcgaa cgtggatgaa ggacttggaa gcgaaccgat
     6481 cttgccggca cttggcttga gacggacatt gcagtcacgc gcgtcaatat tccgtccagc
     6541 tgaaggaaac agtttcccca tacatgcagg cacgaagttg gccgccggcc aagttgacta
     6601 gcaagtcttt cagcgcagat agaactggtg tcggtggtcg catcgaaatg ctggtggtag
     6661 gacagacaca cctcttgaac gtctagggtg ttttgtactt ggccatgata attccacaac
     6721 gagcacactc gaccggcctg cagatgacag tcagaaaccg cgcaaagtca tcctggaaaa
     6781 gactaccgga cagttaatga gcctgcctgc gcagtagagc aaatgcatcg agcatcacat
     6841 ggtaggggta atgggcatta cgtccgctcg gattgggcaa gagaaacacc ggaatcccgg
     6901 caagtcgcgc cggagtagga cccaacgcca ggcggctcga acgggtgatg tccgggaata
     6961 ggatgcgaaa tatggtgacg cccagcaacg cgatcgttcg aggctggtat cgccgaattt
     7021 ttcgttcgag ggcggaggcc cttgcccgat actcgcccgg gtccaggtca tcgattccgg
     7081 cacttggtcg atcgatgatg ttagtgaggc ccagacgcca ttcgggaagt cgccaatcct
     7141 cgcgaaaact cagtggttcg tccaccatgc ccgagtcaaa gagcagcttc cagaagcggt
     7201 tagaatgacc ggcgaagtga tgccccaccg ctgcagatct caaccccggg ttaatgccta
     7261 caaagatgac atgcaagtcc ggtcgtaaat ggtcacggag cctgggtccc gttctctgtt
     7321 tggccatcat ggtactcgtg tcggagctga ggcaatttag cgtgatccgg ctattctgcc
     7381 acagtgttgc tgagagatga gagaccccta ccgtttcctg tagaacgcat aaacgactga
     7441 aattgcaagg agggcaaggc ccgatgggat cgggcaccaa gcttgctgaa ccctgtcacg
     7501 atgcccgctg aagggcatac atttcacaac cagaggaggt gacacagtat gaaacgcatg
     7561 ttgttcgcaa ttatggcagt ggcagtggcg atttctttca gcgctccttc
//
LOCUS       DASTQY010000243         4178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_847943, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000243 DASTQY010000000
VERSION     DASTQY010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847943"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VFR82_08050"
     CDS             complement(<1..621)
                     /locus_tag="VFR82_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501589.1"
                     /translation="MNWTQGIGRGVLSMLFVGICAGYTFAEEQSTGVGKGEASQQKSQ
                     QGIGVEGGGSSHVLLGGPEIIIGKIEQVQGEEYSIQGDHGQSVKLRLTKDTNMVCAGG
                     EGAKMSTGKQDPKEGHEIPISPATEGQMQSHDASKAQQQMQALNDPNQQQTEHQMPAP
                     SKDPSQLKDVVGSTDEAANKDIARGSGFSVGGSSGCQFKQGDMVRVE"
     gene            1027..1326
                     /locus_tag="VFR82_08055"
     CDS             1027..1326
                     /locus_tag="VFR82_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501590.1"
                     /translation="MVRSSKAIFFLLFLAVCAVQITGAACSDDCLRQSSSTPHPAQDS
                     QTEPDGAEGESALELALAQGDGLPVIHLISRCVLDTSLCPRSAFLSDWFRPPTGI"
     gene            1408..2667
                     /locus_tag="VFR82_08060"
     CDS             1408..2667
                     /locus_tag="VFR82_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU4501591.1"
                     /translation="MRRWFIVLFCNMLLVVLSARAGADVSLPTYALADIIGIAVERNP
                     LVAGALAVIDQSTGQRTAAGAYPNPTLHGDGGKGYLRDAGPLTNEIPRSANEYMAGFS
                     QPIEWPAKRAARQRAAEAGLAGASVGLVETRLNLTADVKVAFYDLLLAQRDLDLAKNN
                     LTIVEDVRRIVGARVRLGEAPQFELIKAEVEVLKANQAVTRAENTVRVNRVILDTFTA
                     GALGPAYAVSGDFLVFPTGLDLGQLAARSIHEHPSIQRLARSIDQADRTVEFERQSRV
                     PNVNLNTSYAREYGREGFDVGLSLPAPLWYQRQGEIAGSLGAKRREEAELLRTRNELL
                     RQVNQYFQDAQTTAKLIEVLEKGLLRQAEEALRIAKFSFQQGAASLLEVLDAQRVQRQ
                     TLFDYAQARFELSVALARLERAVGGLL"
     gene            2739..3935
                     /locus_tag="VFR82_08065"
     CDS             2739..3935
                     /locus_tag="VFR82_08065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU4501592.1"
                     /translation="MFNLISRFTQYGCLLMVMFSAGCNSAAEESSGQHSTRKPHETGI
                     VTLTAEEIRSGSVVVEPAVRGEFKLYRDFPATVVPDHHATADITALVRGRVVDVYADL
                     GQQVKGGDLLAMMYSSELGMAQSAYLKAVAKLYVAERAYERATMLLREKVIGLAESQR
                     RQGEMLSLRAEKRESEDRLRLLGMSEEQIAKLNKEQKILSYVPITAPFDGRVITRNLT
                     KGEVVEVTEKLFTVADLSEVWVLANIPEKDITFIRADAGSEKSGVEQLVEVLVTAYPG
                     EVFHGKVTYVGDVLDVATRTMNLRLELPNPQRKLKPEMYATIRVYSLPEPGVLIVPEA
                     AVQRERDRRFVFVQRDAHTFEARDVKLGDGNGEVVKVLEGLQEGEPVVVKGAYVLKSE
                     LLSDQI"
     gene            4015..>4178
                     /locus_tag="VFR82_08070"
     CDS             4015..>4178
                     /locus_tag="VFR82_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU4501593.1"
                     /translation="MSAFLELALRQRVLVLALACLMAAGGIYSFRTIAIDAFPDVTTV
                     LVQVVTEAQGL"
BASE COUNT          885 a         1196 c         1171 g          926 t
ORIGIN      
        1 ttccactctg accatgtcgc cttgcttaaa ttgacatccg ctggagcccc cgactgaaaa
       61 gccggaacca cgagcgatgt ccttgttggc cgcctcatcg gtcgatccga caacgtcctt
      121 caactgtgat ggatccttgg acggcgcggg catttgatgc tcagtttgtt gctggttggg
      181 atcgttcaac gcctgcatct gctgttgagc ttttgaggcg tcatggcttt gcatctgtcc
      241 ttcggtcgca ggggaaattg gaatttcgtg cccttctttc ggatcctgct tgccggtgga
      301 catcttggca ccctctccgc ccgcacacac catgttggtg tctttcgtga gacgaagttt
      361 cactgattgt ccatgatcgc cctggatgga atactcctcg ccctgcacct gctcgatttt
      421 accaataatg atctcagggc cacccaacag aacatgtgac gaaccccctc cttctacccc
      481 aatcccctgt tggctcttct gttgagatgc ttctcctttt cccaccccgg tcgattgttc
      541 ctcggcaaag gtataccctg cacaaatacc cacaaacagc atgctcaaca caccccgtcc
      601 tatcccttgt gtccagttca tcggctccct cctttcgaaa gtgacattgt ggtttctttg
      661 ccaggttgcc ccgtcagact atgctgcctt aaggcaaggg actcgcaaaa cacgggcctt
      721 acgaagtagc attggaagcg tggataatgt gagagaaaaa tgccgttcag tcgctggatg
      781 ctggtcagac tgtcttacgt aggacatcgc gtcctagcat ctggttcgat caatcattcg
      841 atcaatgata ggacgcagca acaagtccaa cgactcggga tacccccagt atagaaagcc
      901 tagacgacat gggatcttta gatactcccc tgtgtttccg cttggtttga atggataacc
      961 gaggcaattg cggatgtggt actcatcatg gtacggtgag gccgacttct gtggggccag
     1021 atgtccatgg tgcgttcctc taaggctatt ttctttctcc tctttctcgc ggtttgcgcc
     1081 gttcaaatca cgggagcggc gtgctccgat gattgccttc gccaatcgtc ctccacgccc
     1141 catcccgcac aggacagcca gacagagccg gatggcgcgg aaggggaaag cgcgttggag
     1201 ttggccctgg cccagggaga cggcttgccc gttattcacc tcatcagccg ttgcgtcttg
     1261 gatacgtcat tgtgtccgcg aagcgctttt ctgtcagact ggtttcgccc tccgacgggc
     1321 atctgaccgc acactcggat accatcttca acattttcaa cattctgagg cgccgctgtt
     1381 ccactctgga acggagggag gcttgcgatg agacgttggt tcattgtcct gttctgcaat
     1441 atgctcctcg tcgtactcag tgcacgcgca ggggcagacg tatcacttcc cacatacgcc
     1501 ctcgctgaca tcatcggtat tgcagtcgag cgcaatccgc tcgttgcggg cgccctagcg
     1561 gtaattgatc agagcaccgg ccagcggaca gcggccggtg cctacccgaa cccgaccctc
     1621 cacggcgatg gtggaaaggg ctatttgcgt gacgccggtc ccttgacgaa cgaaataccg
     1681 agatcggcga acgagtatat ggccggattc agtcagccga ttgaatggcc ggccaaaagg
     1741 gctgcccggc agcgggcggc agaggcagga ctggccggcg catcggtcgg tctcgtcgag
     1801 acccgcttaa atttgaccgc cgatgtgaag gtggccttct atgaccttct tttggcgcaa
     1861 cgcgatttgg atctcgcaaa gaacaacctc acgatcgtcg aggatgtgcg tcggatcgtc
     1921 ggcgcacgtg tgaggttagg tgaagcgccg caatttgagc tcatcaaagc ggaagtagaa
     1981 gtccttaagg caaatcaggc cgtgacgcgc gcagaaaata cggtccgtgt gaaccgcgtc
     2041 atcctggaca ctttcactgc gggcgcgctc ggtcctgcct acgccgtttc tggagatttt
     2101 ttagtgtttc cgaccggcct cgatctcggg cagctcgccg ctcggtcgat ccacgaacac
     2161 ccttccatcc agcgtctcgc ccgctcgatt gaccaggcag atcggacggt ggaattcgag
     2221 cgtcagtcac gggtacccaa cgtgaatttg aacacgagct atgcccgcga atacggccgt
     2281 gaaggcttcg acgtaggact cagccttccg gcgcctctct ggtatcaacg ccagggagaa
     2341 atcgccggct cgctgggtgc aaaacgaagg gaagaggcgg aactccttcg cacgcgcaat
     2401 gaattgctta gacaggtgaa ccagtacttt caggatgcac agacaactgc caagctgatt
     2461 gaagtcttgg agaaggggtt gttacggcag gcggaagaag ccttgcgcat cgccaaattc
     2521 agttttcagc agggcgccgc gagtctgctg gaagtcttgg acgctcagcg ggtgcagcgg
     2581 caaaccctgt tcgactatgc gcaggcacga ttcgagctgt ctgtagcctt ggcgagattg
     2641 gaacgtgcag tgggaggcct tctctaaatc gatgtgagtg tggaccgctg agtcatccac
     2701 tcggcagcct gcactcaagc gtaggactta ggaatcatat gttcaacttg atctcacgct
     2761 tcacacagta cgggtgcctg ctcatggtga tgttttcagc aggctgcaac agcgcggcgg
     2821 aggaatcgtc cggccaacac tctacacgca aacctcatga aacgggaatc gtcacgctca
     2881 cggccgagga aatacggagc ggatctgtcg tcgttgaacc ggccgtccgt ggcgagttca
     2941 agctgtaccg agatttcccg gccacggtgg tgccagatca tcatgccacc gcggatatca
     3001 ctgcgctggt ccggggtcgc gtcgtggacg tttatgccga tttgggacaa caggtcaagg
     3061 gcggcgacct cctcgccatg atgtacagca gcgaacttgg gatggcgcag tcggcctatc
     3121 tcaaggccgt cgccaaacta tacgtcgccg aacgcgcata cgagcgcgcg accatgctct
     3181 tacgagaaaa ggtgatcggg ctcgccgaat ctcaacgtcg tcaaggggaa atgctcagct
     3241 tacgcgcgga aaaacgggag agtgaagatc ggctgcgcct cttgggcatg agcgaagagc
     3301 aaattgccaa gttgaacaag gaacaaaaga tcctctccta cgtgccgatt accgcaccgt
     3361 tcgatggcag agtgatcaca cgcaatttga ctaagggaga agtcgtcgaa gtcaccgaga
     3421 agttgttcac ggtcgccgat ctttcagaag tctgggtgct cgcgaatatc ccggaaaagg
     3481 acatcacctt catccgcgcc gacgccggaa gcgagaagtc aggagtggag cagctggtag
     3541 aagtactggt gaccgcatat cccggtgaag tcttccacgg aaaagtcact tacgtcggcg
     3601 acgtcctgga cgtggccacg cgcacgatga acctgcgcct ggaattaccc aacccacagc
     3661 gtaagctgaa gccggaaatg tatgccacga tccgtgtcta ctccttaccg gagccaggcg
     3721 tgctgatcgt gcccgaggca gcggtgcaac gcgaacggga ccggcggttt gtattcgtcc
     3781 aacgcgacgc ccataccttc gaagcacgcg acgtcaagct gggcgacggc aacggcgaag
     3841 tcgtcaaggt gcttgagggc ctccaagagg gcgaaccggt tgtcgtcaaa ggggcctatg
     3901 tgctgaaatc cgaactgctt tccgatcaaa tctagcgagt ccgaaaccgc ttcatgcgag
     3961 acactgtgtt caacgcccac gctccttgta tgttttccac cggcctaatc acccatgtcg
     4021 gcttttcttg aattagccct tcgacagcgg gtgttggtcc ttgctctggc ctgccttatg
     4081 gccgccggcg gcatctattc gtttcgaacg atcgcgatag acgcctttcc ggacgtcacc
     4141 acggtcctcg tccaagtcgt gacggaggca caaggact
//
LOCUS       DASTQY010000244        20153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_405575, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000244 DASTQY010000000
VERSION     DASTQY010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 20153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20153
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405575"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFR82_08075"
     CDS             <1..370
                     /locus_tag="VFR82_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501594.1"
                     /translation="FFYPRAGDYAAQAKGATSLDTMINLTNMMEVTAAKAKGGKGKDA
                     ALDDYHNQFHALLDSYCDVPKEQTKTPAYDMAVTHKKELTAIFWRLWKFKDDQPQRDQ
                     HLDLSIAELKELRETLQTIK"
     gene            398..820
                     /locus_tag="VFR82_08080"
     CDS             398..820
                     /locus_tag="VFR82_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4501595.1"
                     /translation="MATNFGHIELITQDLDKAKDFYQKLFGWKARDLPDLPYSLFNEH
                     LFPTGGMMKSPQPGSESFWIPYIVTDDISGVTEKARSLGATIHREVMVAGQYGWLSII
                     NDPTGATFGLWQPKMEPPPPRKKPTAKRTKTKSKRRRR"
     gene            1052..1534
                     /locus_tag="VFR82_08085"
     CDS             1052..1534
                     /locus_tag="VFR82_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501596.1"
                     /translation="MKQLLAGIALFLSMAVAVGSTPVAAESETKNLWQLAPPQPDPPA
                     SQRKPWVLRERDITLDMQLLRILKDPTARPHPRVTLEFFDGHRYEVDITSTVLRINDT
                     AVIRGTFRPPSQGEFTLVASGSLLVGSLQVGSRLYRTEHVANGRLRLMELDPSKAPPE
                     "
     gene            1597..2151
                     /locus_tag="VFR82_08090"
     CDS             1597..2151
                     /locus_tag="VFR82_08090"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEU4501597.1"
                     /translation="MATALPEPQLWTIGHSTKRIEDFLELLRSHRIHRLVDVRTIPRS
                     RHNPQYDTEELAAHLKKVKLTYLHMPQLGGLRKAKKDSVNIGWKNASFRGYADYMQSE
                     QFQLALEQLMAESRERPTAVMCAEAVPWRCHRSLIADALLIRGWAVRHIMTKTKADTH
                     RLTRFAVVEADRITYPSVSFSAES"
     gene            2157..2579
                     /locus_tag="VFR82_08095"
     CDS             2157..2579
                     /locus_tag="VFR82_08095"
                     /inference="COORDINATES: protein motif:HMM:NF022317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEU4501598.1"
                     /translation="MGYGLLADVIALVHLLFVLFVMFGALFALRWPGAIWVHAPALIW
                     GLIVEFADLVCPLTPLENRLRVLSGEAGYREDFLSHWLLTVLYPEFLTRDLQFFLGGS
                     LLFMNIGLYAYVLRKRSNRRPTKRKEAYPCTGDPAGPR"
     gene            2689..3726
                     /gene="arsS"
                     /locus_tag="VFR82_08100"
     CDS             2689..3726
                     /gene="arsS"
                     /locus_tag="VFR82_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_274671339.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HEU4501599.1"
                     /translation="MPSLLARRSPLAPTTAQLQLLEATTACEPFDVPMEQAGLRPLKA
                     TGITVLQLNVGKLCNQTCRHCHVDAAPDRTESMSRETAALCIEALAKTEIPTVDITGG
                     APELNQNFRWLVEQARSLQRHVMDRCNLSVLLLPAQADLAEFLAAHHVEIVASLPYYK
                     MHQTDAQRGDGVFKKSIEGLQRLNRLGYGRPNSGLVLNLVYNPVGAFLPPKQDAIESQ
                     FRKELHKDHGIEFNRLYTITNMPISRFLEFLVESGNYDGYMERLANAFNPAAAAGVMC
                     RYTLSVAWDGTLYDCDFNQMLNLPVDQRAPRHIRDFNPLALNDRSIVTGNHCYGCTAG
                     PGSSCGGSVTV"
     gene            3832..4866
                     /gene="hflK"
                     /locus_tag="VFR82_08105"
     CDS             3832..4866
                     /gene="hflK"
                     /locus_tag="VFR82_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEU4501600.1"
                     /translation="MVWDPKDPWSRKSDPLEDALKQAQLQFKNLLPSGGFRNILLAGA
                     LIFLAWQSAFIIGPDEEGIVKRFGIPVRSVGPGPHVKIPLVESVLQPKVAKLHRVEIG
                     FRTDRQSRQQMIPQEALMLTGDMNILAIEFIVQYKIKEAANFLFNVADIHDTIGKAAE
                     ASMREVIGKSKIDEALTTGKAQIQQDTQDLLQKILDQYQAGVQIAAVQLQDVDPPEAV
                     AAAFKDVTNAKEDREKLINQSIGYRNDILPKAKGEAAQIVNQAKGYAQARLNRAQGEG
                     NRFLATLHEYNQARDTISKRIYIETMEEILPKMEKIIIDGKAAERLLPYLPIDRFSKK
                     PPVSAEGRQP"
     gene            4863..5723
                     /gene="hflC"
                     /locus_tag="VFR82_08110"
     CDS             4863..5723
                     /gene="hflC"
                     /locus_tag="VFR82_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEU4501601.1"
                     /translation="MSKQGLTIAVIAVFIGLLVLGASPLFVVDVTQTAIVVQLGKPVR
                     TVMEPGLYVKVPFVQEVTYFDKRLLDYDSDAQDVITQDKKTLLLDNFAKWLINDPLKV
                     YQNFQSQRGALQRLHDIIYSELRVELGRHDLAEIVSTARAELMKVVTQRANEKASAYG
                     ITIQDVRIKRADLPQENEKTVFQRMQAEREQQAKQYRAEGAEEAQKIRSDAEKDREII
                     LAQAYKESEELRGSGDAKAFRIYADAYRQDPKFFDFTRSMEAYKKSFDDKSTVVMTPD
                     SEFFRYLKQR"
     gene            complement(5728..7410)
                     /locus_tag="VFR82_08115"
     CDS             complement(5728..7410)
                     /locus_tag="VFR82_08115"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU4501602.1"
                     /translation="MTDLEIARAYRPLPIQDIAKRLGLSPDELHPYGRYKGKVALQAL
                     DRLKGKPEGKYILVTAINPTPLGEGKTTTSIGLAMGLCRVGTRAVVTLRQPSLGPVFG
                     IKGGGTGGGRAQVFPMEEINLHLTGDAHAVSASHNLLSAFIDNHLFHGNALGIDPARL
                     SWPRSLNVSDRALRHISLDESARCRPASFVITEASEVMAILALSSSQADLRRRLGQIV
                     VGVSTGGRPVRAEDLSCAGSMALLLKEALLPNLVQTLEGTPAFVHTGPFGNIAHGNCS
                     VISDAMALRCADYVVTEAGFGSDLGAEKFFNIKCRLSGFVPAAAVVVATLRALKLHGG
                     GGVVKAGSPVPVSLSGPNQEALARGFANLEQHIANVKAHGIPVVVAVNAFKDDPPSEL
                     EWLRAQACKAGAAEAAISTHYADGGKGSEELARAVIRAAELRASFSCLYDQAWPTKKK
                     IETIATKMYGASGVSFEPQAEEDMELATQLGYERLPVCMAKTPLSLSHDPALKGRPSG
                     FTLPIKEVRLLAGAGFLTAVCSGIQLMPGLPKKPAGERIGLDPQTGETVGLS"
     gene            complement(7536..8555)
                     /locus_tag="VFR82_08120"
     CDS             complement(7536..8555)
                     /locus_tag="VFR82_08120"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU4501603.1"
                     /translation="MGQVSGVESNDSYTVVIFRGSTAKPLRFSFPRKFVRKILILAAI
                     LLAADVLVISHYVVRTGEVWELSAFRSEALSARQQTAAFSTAVDDLKKRLTSMKEVNQ
                     RLRVMLGIEMPKTGDQLNGRGGEETPLPEGSTLNSPDKLIRSGAVPQQPNAVDGLIEL
                     NTSKSTDLDEQELVAAVREGIVWLAKEADVQEHTLQELALAAEQKSSRWAATPSIWPV
                     KGWVTSGFGPRVSPFTEKPAWHDGLDIGAAANAPVQAPAQGRVTNVGFDSKLGNVVKL
                     DHGFGIETLYGHLAKALVKEGQRVKRGDVVGLVGSSGLATGPHLHYMVKVNGQTFDPS
                     KYILD"
     gene            9382..9702
                     /locus_tag="VFR82_08125"
     CDS             9382..9702
                     /locus_tag="VFR82_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU4501604.1"
                     /translation="MGYTITPTELKSRLDKGEQLVLLDVREQWEFDLAKLNGSTLIPL
                     ATLPQSLGKLNRETEIVAICHHGMRSADATNFLLQQGFSNVKNLVGGIDAWSAQVDRS
                     VPRY"
     gene            complement(9737..10660)
                     /locus_tag="VFR82_08130"
     CDS             complement(9737..10660)
                     /locus_tag="VFR82_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU4501605.1"
                     /translation="MKVLVTGGAGFIGSHLVDRLVMEGHEAVVVDNLITGKRRNINRA
                     ARFYKMDIQSWRLERVFRNERPNVVMHLAAQMDVRKSVEDPMFDAQVNILGTLNVLQQ
                     AVKHGVRKVIFSSSGGAIYGEQETYPAPETHVLKPLSPYGLSKLCGEQYLSYFQRVSG
                     LQAVSLRYANVYGPRQDPEGEAGVVAIFIQKMLNNEQPVINGNGRQTRDFVFVDDVVE
                     ANLAMIGQETQGTYNVGTGVETSINDLFRILVQHTGSVCKEIHGPAKKGEQARSVIDN
                     TKLRQEQSWEPKADLVEGLKKTVEYFQSRAH"
     gene            complement(10689..11456)
                     /locus_tag="VFR82_08135"
     CDS             complement(10689..11456)
                     /locus_tag="VFR82_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU4501606.1"
                     /translation="MNSKAPVGKERLDRLLVSHGLVSSREVAVRTILAGGVLVDGTVV
                     DKPARLVPRGARIEIERAAPFVSRSGEKLAAGLDAFSADPRHLVGLDVGCSTGGFTDC
                     LLQRGIKRVYAVDVGYGLIDWKLRQDSRVILLERTNIRYVDRSLIPEPIDLAVIDVSF
                     ISLTVVLPSVVPLLREGARVIALIKPQFEVGKGQVGAGGIVRDDGQRRAVTERVIECA
                     ARLGLHSTGTLDSPVKGRKGNREILVSFLRRGIIASS"
     gene            complement(11522..11782)
                     /gene="xseB"
                     /locus_tag="VFR82_08140"
     CDS             complement(11522..11782)
                     /gene="xseB"
                     /locus_tag="VFR82_08140"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU4501607.1"
                     /translation="MGGVKFEQAMARLEAIVGELEKGDLPLDESLKIFEEGIRLSKSC
                     LKILEDAERKVEVLVQDRNGKKRLHAFSPDEEAEESADESSC"
     gene            complement(11839..13266)
                     /gene="xseA"
                     /locus_tag="VFR82_08145"
     CDS             complement(11839..13266)
                     /gene="xseA"
                     /locus_tag="VFR82_08145"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU4501608.1"
                     /translation="MLESARRCQSNGSGSLTRMSATRQQTLFHPSKSVLTVSQLTGLL
                     RTTVEGQFSDVWLEGEVSNLRMPGSGHIYCTLKDESSQIRAVLFRSSALRLKFTLQEG
                     MCIIVRGRLTVYEPRGEYQIVLETVEPKGIGALQLAFEQLKARLAAEGLFDEARKKPL
                     PAFPSSIGVVTSPTGAAIRDMLSVLHRRWPILHIILVPVPVQGEGAGQHVADAVALLN
                     EQAVADVIIVGRGGGSMEDLWSFNEEVVVRAVAASRIPIVSAVGHETDVTLTDFAADR
                     RAATPSAAAETVVPVLADVVERLRALTVRNGHAMARYCVLEQRRLDASINGLAQVQLR
                     IRREWQRTDGTIGRLKTLVCERVAVSRDLVRQHQRELAGLNPMFYVKRGLSLIPTLVH
                     RLERQILVLNETRKRRIAAIAAQLTHLSPLAILGRGYSILMRARDGTILRSALDVEVD
                     EELVARLAQGELDCTVKRVVPDPLV"
     gene            complement(13214..13993)
                     /locus_tag="VFR82_08150"
     CDS             complement(13214..13993)
                     /locus_tag="VFR82_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEU4501609.1"
                     /translation="MKVLYIGDIMGEPGRRAVARAVPRLVSQRQIDVVVGNGENVAGG
                     FGITPDLADELFGMGLSAITTGNHAWDKKEILDYFPRERRLLRPANYPEGVPGQGSVV
                     IRTKNGEEVAVLQLMGRAYMPTLDCPFQVAKREMSRLRKLVAAVLVDMHAEATSEKMA
                     MGHYLDGEATAVVGTHTHVQTADEQILPRGTAYLTDIGMTGPLHGVIGVKKELAIEKF
                     LTGMPRRFEVAGGPTVFCAALIELDARIGKALSIERIRFTD"
     gene            complement(13990..15558)
                     /gene="rny"
                     /locus_tag="VFR82_08155"
     CDS             complement(13990..15558)
                     /gene="rny"
                     /locus_tag="VFR82_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU4501610.1"
                     /translation="MSTSIVMYILVGLVGALLGAGLFELARRSSATAKRAQEEDQARQ
                     TIQNAQREAENIVKEAKLEAKDLVFQSKAELEKEQKAKLLELSITEKRVVQREEAIDK
                     RISVLDKRDADCQKRDQELIRKEEKLIAKEAACAQAEREHREALERVAGMTADEAKRH
                     LLQEMESQARLDAAGIAKRTLEEARENAEREAREIITTSIQRVVRDYVSESTISVVPI
                     PNDAMKGRIIGREGRNIRAIEAATGIDLIIDETPEAVIISGFDPLRREIAKVSLERLM
                     HDGRIHPTRIEEIVEKVKVDIDKLMYEEAEKIIFELGLSDFHPELIKVLGRLKYRTSY
                     GQNNLYHAREASYICGIMASELGLDVRLARRGALLHDIGKAVSHEEEGPHAMLGAEIA
                     KKYGESEKIVNAIAAHHEQVEPICPESVLVAAAEALSAARPGARREALESYVKRLEKL
                     ESLAHAQKGVQKAYAIQAGREIRVIVRQEDVTDSESFQLSRELAKKIEQELTYPGQIK
                     VTVIRESRYIEYAK"
     gene            complement(16130..16426)
                     /locus_tag="VFR82_08160"
     CDS             complement(16130..16426)
                     /locus_tag="VFR82_08160"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU4501611.1"
                     /translation="MTKTTEVEIYGQRYSIRGEADETYIRRLAGFVDGQMRQLAEGMN
                     TTTPSRLAVLTALNLAHQLLEAEKKRAQGEADVERRMTSLMESIEEQVPTSLFR"
     gene            complement(16419..16673)
                     /gene="zapB"
                     /locus_tag="VFR82_08165"
     CDS             complement(16419..16673)
                     /gene="zapB"
                     /locus_tag="VFR82_08165"
                     /inference="COORDINATES: protein motif:HMM:NF017786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HEU4501612.1"
                     /translation="MALDRLDALETRIKDLVKLIQELKKRNAGLEDDLRSTRQRLADE
                     SDSNRRWARERSDIKARVEKVLGDIELLEAFEERKEVAFD"
     gene            complement(16748..18973)
                     /locus_tag="VFR82_08170"
     CDS             complement(16748..18973)
                     /locus_tag="VFR82_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU4501613.1"
                     /translation="MVAVLVRFCLCGLLLLINGADGTAALPGEADPCEQPELCFQSAA
                     FPKERLGKTMNKEQVLTLKLERLQHLADRYSGTLWGKRAGLLSGVLLIERDPASAMQF
                     LRTAQRDFSVLDDYIRLWIAESQLNLGQAREAAVLLESIPMAVPDSNILGRAAYKTGE
                     AWYQAAACAESITWFGRAVAMNDKDTGASQAYLRRGACRLRESNVVEGRELLQQVWVR
                     FPYTREAKEAETLLTSNLGGETWTVQPADRLNRAQAFLGQAYHAEAIEELKKYLLADP
                     HSPRRAEAKLKLGIAQVRLKLYDQARETFRGLTKDGGPESREAAVWLARVYLRQGQGE
                     KLLEAARALASSSLSPEQKGQITLFAGMWLEDQKQFDEAIGKYVHVAQHGEPAAQRAE
                     GQWRVGWVYYRTGRYREAGESLRALADQHDSEFEPQALYWIGRAGEMNNQPLEGQDFY
                     RKLCQRHPFTYYCQLARERMKIPPVADAPTEAMVLSSDTAKTGDPARPTVTRADIEQH
                     TAYRRAIELKTLGLDADAAHEVAVLTERYSRDPDVLIALSTMLNEVGAYHQALRLARA
                     RFRDQLERTGGAFTPSLWNVAYPTGLLPTIKLQGAKGVDPYLIAAIIREESQYDVKAV
                     SRVGAIGLMQVMPATANNVAQRVGLPALGREDLFDQETNIRIGVRYVEQLLEQFSGNL
                     VYTVASYNAGPLAVGNWIAQYRGHNQDEFVELIPYQETRQYVKRVLRSYREYVRLNGL
                     S"
     gene            19096..>20153
                     /gene="rlmN"
                     /locus_tag="VFR82_08175"
     CDS             19096..>20153
                     /gene="rlmN"
                     /locus_tag="VFR82_08175"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU4501614.1"
                     /translation="MRGVKTMAPLTTINLLTLDESETAALVRSLGWPSYRNGQILRWL
                     YQRRVRSISQMTDLSQADRARLGSEARIGRALGCTVFQSADGTRKLILKLEDDLEVEA
                     VLIPDDERLTLCVSTQVGCTLDCGFCLTGTMGLKRNLKAHEIIEQVLTAHDHLNPGER
                     LSNMVFMGMGEPLANLAALSEAVRRLTNTKWGLGWSPRRITVSTAGLASKLHEVAPLG
                     VNLAISLNATTEEQRARLMPAVHGIASLKTLLSACRRFPLARQRRLTFEYVLLAGVND
                     RELDAKRLVALIKGINCKVNLIPFNPFPGSPFRRPSDRDILTFQSIVRQAGVDVFIRK
                     SRGRDVLGACGQLGNLPPS"
BASE COUNT         4398 a         6032 c         5396 g         4327 t
ORIGIN      
        1 tttcttttat ccacgcgcag gagattatgc ggcacaggcg aaaggcgcga ccagcctcga
       61 cacgatgatc aacctgacca atatgatgga ggtgacagcg gcaaaagcga agggcgggaa
      121 aggcaaagat gctgccctcg acgactacca taaccagttc catgccctcc tggactccta
      181 ttgcgatgtg ccgaaagaac aaacaaagac tcccgcctac gatatggcgg taacccacaa
      241 gaaggaattg accgccatct tttggcgtct ctggaaattc aaagacgatc aaccgcaacg
      301 cgatcagcat ctggatctct cgatcgcgga actgaaagag ctgcgggaga cgttgcaaac
      361 gatcaagtaa gcggctgagc agcgcgggag gcaactcatg gcgaccaatt tcgggcacat
      421 cgagctgatc acccaggacc tcgacaaagc gaaagatttc tatcagaagc tgttcggctg
      481 gaaagcccgc gatctaccgg atctgcctta ctcgctcttt aatgagcacc tgtttcccac
      541 gggcggcatg atgaagagcc cgcaaccagg atcagaatcc ttttggatcc cctacatcgt
      601 aacggacgac atcagcggtg tgaccgagaa ggcccggagc ctcggtgcca cgatccatcg
      661 cgaggtcatg gttgcgggac agtacggctg gctgagcatc atcaacgatc ccacaggagc
      721 cacatttggg ctttggcaac ccaaaatgga gccgccgcct ccgagaaaaa agccaacggc
      781 caagcgcacg aaaacaaaat cgaaacgccg acggcgctaa gcacggcaca acctgacacc
      841 gcagtccgca gacctctcaa cggtctcact tgccttgcca tgctggaagc catgactcgc
      901 agcgcaagga atgctctttg ggattacgag gcgccggttg agatccttga aatcgtcgga
      961 gcatttggtc gaatgggcga tccaggttct cgcgccagct gcacgatgag ccctgcatca
     1021 ttaacgtgat gatgtttttc aggaaggctc aatgaaacaa ctgcttgctg gtatcgcatt
     1081 gttcctcagt atggccgtcg ctgtcggatc cacaccggtc gctgccgagt cggaaaccaa
     1141 gaatctctgg caacttgcgc cgccacagcc ggatccgcca gcatcgcaac gaaagccatg
     1201 ggtgctccgt gagcgagaca tcaccctcga catgcagctt cttcgaatat tgaaagatcc
     1261 aacagcgaga ccgcacccgc gggtgaccct cgaattcttc gacggacatc gctatgaggt
     1321 ggacattacc tcgaccgtgt tacgcatcaa cgataccgcg gtcatccgcg ggacgtttag
     1381 accgccttcc caaggcgagt ttacgttggt ggccagcggc agtctgctgg tcggcagtct
     1441 gcaagtggga agccgcttgt acagaacgga gcacgtggcc aacggcaggc tcaggcttat
     1501 ggaactggac ccgagtaaag ctccgcccga atagcacccg ttcactatct tgatctggtc
     1561 tgaattcttc atccagaatg gagaggggtg caggcaatgg cgaccgcgtt acccgaaccg
     1621 cagctctgga ccatcggcca ttcgacgaaa cgcatcgaag acttcctgga gctgctgaga
     1681 tcccaccgca ttcaccgtct cgtcgatgtg cgaacgattc cccgttcgcg acataatccg
     1741 caatatgata ccgaggaatt agcggcgcat ctgaagaagg tgaagctgac ctaccttcac
     1801 atgcctcagc tcggcggttt gaggaaagcc aagaaagatt ccgtgaacat tggctggaag
     1861 aacgccagct ttcgagggta tgccgactac atgcagtcgg aacagttcca gctcgccctc
     1921 gagcaactga tggcggaaag tcgtgaaagg ccgaccgccg tcatgtgcgc ggaagctgtg
     1981 ccatggcgct gtcaccgatc gttgattgcc gatgccttgc ttatccgggg atgggcagtt
     2041 cggcacatca tgacgaagac gaaggctgac acccatcggt tgacgcggtt tgccgttgtc
     2101 gaagcagatc gcatcacgta tccatctgtc tcattttccg cagagtcttg aagcgtatgg
     2161 ggtatggcct cctcgccgat gtcatcgcgc tagtacatct tctcttcgta ttgtttgtga
     2221 tgtttggtgc tctattcgca cttcgatggc ccggcgctat ctgggtgcat gctccggcgc
     2281 tgatctgggg actcatcgtc gagtttgccg atctcgtctg tccactcaca ccgctggaaa
     2341 accggctacg tgtcctatca ggtgaagccg gctacaggga ggattttctc tcgcattggc
     2401 tcttgactgt cctctatcct gaatttctga ctcgggatct ccagttcttt ctcggtggat
     2461 cactcctatt catgaatatc ggcctctatg cctatgtttt gaggaagaga tcgaatcggc
     2521 gaccaacgaa gagaaaagaa gcctacccct gcaccggtga tccggctgga ccgcgctaag
     2581 ctgaaaggct ctgacgattg ttgacctgcg cgtgaatctc tggctagatg agtaaggcga
     2641 actgcgtatg gaaaggcatc tctcggacca cattgacaga gccagctcat gccatcactc
     2701 cttgcacgac gaagccctct agctcctacg acagcgcagc tgcagcttct ggaggcgact
     2761 accgcttgcg aaccatttga tgtcccgatg gagcaggccg gcctccgccc actcaaagcg
     2821 acgggcatta ccgtccttca gctcaatgtc ggaaaactgt gcaatcagac ctgtaggcat
     2881 tgccatgtag acgcggcacc cgaccgcacg gagagcatgt cccgtgagac ggcagcactc
     2941 tgcatcgagg cgttggccaa aaccgagata cctaccgtcg acattaccgg aggagcgcca
     3001 gaactcaacc agaatttccg ctggctggtc gaacaggccc ggagcctaca gcgacatgtg
     3061 atggaccgct gcaacctttc ggttctcctg ctgccggctc aggcggattt agccgaattc
     3121 ttggcggctc accacgtcga gatcgtggcg tcccttccct attacaaaat gcaccagaca
     3181 gacgcccagc gcggagacgg cgttttcaag aagtcgatcg aggggctcca gcgactgaat
     3241 cgactcggct acggacgacc caatagcggg ttggttttga atctagtgta caatccggtc
     3301 ggcgcgtttt tgcctccaaa acaggacgcc attgaatcgc agtttcgcaa agagttacac
     3361 aaggatcacg gtattgagtt caatcgtctc tatacgatca ccaacatgcc gatcagccgc
     3421 ttcttagaat ttctggttga gagtgggaac tatgacggct acatggagcg ccttgctaac
     3481 gccttcaatc cagccgccgc agcaggcgtc atgtgccgct atacgttatc cgtcgcgtgg
     3541 gacggcacgc tgtacgattg cgattttaat cagatgttga acctccccgt cgatcaacgg
     3601 gcgcccaggc acatacgaga tttcaatcct ctggcgttga atgaccgatc aatcgtcaca
     3661 gggaatcatt gttatggctg caccgccggc cccggctcgt cgtgcggagg ctctgtgacc
     3721 gtctagccgc gctgcacccc gctccgtcag cactcagcgt gttgcgaagg cacgggcact
     3781 ctgctatggt gtcgcccgtt ctcaggtagt aaaccttccg caaatcgccg catggtctgg
     3841 gatcccaaag acccgtggag cagaaaaagc gacccgcttg aagatgcgct caagcaggcg
     3901 caactgcaat tcaagaacct gcttcccagc ggcggattca gaaacatcct cttggctggg
     3961 gccctcatct ttctagcctg gcaaagcgcc ttcatcattg gaccggatga agaaggcatt
     4021 gtgaagcgct tcggtattcc ggtgcggtca gttggtcctg gccctcatgt caagattcca
     4081 ctcgtcgagt cggtgctgca gccaaaagtc gcgaaacttc accgcgtgga aatcggcttt
     4141 cgcacggatc gccaaagccg gcaacagatg attccacagg aagccctcat gctcaccggt
     4201 gatatgaaca ttctagccat cgaattcatc gtccagtaca agatcaaaga agccgcaaac
     4261 ttcctattca acgtcgccga tatccatgac accatcggca aggcggctga agcgtccatg
     4321 cgagaagtca tcggcaagag caagatcgat gaggccctga cgaccgggaa agcgcagatc
     4381 cagcaggaca cccaggatct gctccagaag attctggatc agtatcaggc cggtgtgcag
     4441 atcgccgccg tgcaactcca ggatgtggat ccacctgaag ccgtggccgc cgcctttaag
     4501 gatgtcacca atgcgaaaga agaccgtgaa aagctgatca atcagtcgat cggctatcgc
     4561 aatgacatcc tgccgaaagc gaaaggtgaa gcagcacaga tagtgaacca ggccaagggc
     4621 tatgcacaag cgcgcttgaa tcgagcccag ggagaaggca accggttcct ggccaccctg
     4681 cacgaataca atcaagcgcg ggataccatc agcaaacgca tttacatcga gaccatggaa
     4741 gaaatcctgc cgaagatgga aaagattatc atcgatggaa aggccgccga gcggttgctc
     4801 ccctacttac ctattgaccg gttctccaag aagccccccg tatcggcgga aggacgccag
     4861 ccatgagcaa gcaggggctg accattgcgg tgatcgccgt ctttattggt ctcttggtgc
     4921 tgggcgcctc gccgctgttt gtggttgatg tcacccagac ggccatcgtc gtgcaactag
     4981 gcaagccggt tcgcaccgtc atggaacccg gcctctacgt caaggtgcct ttcgttcagg
     5041 aagtgaccta cttcgacaag cgccttctgg attacgattc ggatgcccaa gacgtcatca
     5101 cgcaggacaa gaaaacgttg ctgctcgaca atttcgccaa gtggctgatt aacgatccat
     5161 tgaaggtcta tcagaatttt caaagccagc gaggggcgtt gcagcggctt cacgacatca
     5221 tttattccga gctgcgggtg gaattgggcc gacatgatct agccgagatc gtatccacgg
     5281 cgagagccga gctgatgaag gtcgtcaccc agcgggccaa cgaaaaggcc tcggcctatg
     5341 gcatcacgat tcaggacgtg cggatcaaga gggcggatct gccgcaagaa aatgaaaaaa
     5401 cggtctttca acggatgcag gcggaacggg agcaacaagc caagcaatat cgtgctgaag
     5461 gcgcggagga agcccaaaag attcggtcgg acgcggaaaa ggaccgtgaa atcattctgg
     5521 cgcaagccta taaggaatcg gaagagcttc gcggttccgg cgacgcgaaa gccttccgga
     5581 tttatgccga cgcctaccga caggatccta agttttttga ctttaccaga tctatggaag
     5641 cgtacaagaa gagcttcgat gacaagtcca cggtcgtcat gaccccagac tcagagtttt
     5701 tccgctatct caagcagcgc tgatccgtca ggagagcccg accgtttcac cggtttgcgg
     5761 atcgaggcca atgcgttctc cggccggttt cttcggcaac cccggcatca actggatgcc
     5821 ggagcagaca gccgtgagaa acccggcgcc tgcaagcaat cgcacctctt tgatgggcaa
     5881 ggtaaacccg ctgggacgac ctttcagcgc cgggtcgtgc gaaagcgaca gcggcgtctt
     5941 ggccatacac acaggcagtc gctcgtatcc caactgcgtg gccagttcca tgtcctcttc
     6001 agcctgcggc tcgaatgaga cccctgacgc gccgtacatc tttgtggcta tagtttcgat
     6061 cttcttcttt gtcggccaag cctgatcata taagcaggaa aatgatgctc gcagctcagc
     6121 ggcgcggatc accgcccgcg cgagttcttc cgaccctttt ccgccgtccg cgtaatgggt
     6181 ggaaatcgcc gcttcggccg cgccggcctt acatgcctgt gctcgcagcc attccaattc
     6241 tgaaggcgga tcgtccttga acgcgttgac ggccacgacg accggaatac catgtgcctt
     6301 gacgttggca atatgttgtt ccagatttgc gaatcctcgc gcgagcgctt cctggttggg
     6361 tccggaaagg ctgaccggga cgggggagcc tgctttaacc acgcctcccc caccatggag
     6421 tttgagcgcg cgtaacgtcg caacgaccac ggccgccgcc gggacgaacc ccgacagtcg
     6481 gcacttgatg ttgaagaatt tctcagcgcc gagatcgctc ccaaacccgg cttctgtgac
     6541 cacataatcg gcgcagcgca aagccatggc gtcagagatg accgaacaat taccatgcgc
     6601 gatattgcca aacggaccgg tgtgaacgaa tgccggtgtc ccttcgagcg tctgaaccag
     6661 gttaggcagt agggcctcct tcaggagcaa ggccatggag cccgcgcacg ataagtcctc
     6721 cgctcgaacc ggtcttcccc ccgtgctcac cccaaccaca atttggccaa gccggcgtct
     6781 caagtcagcc tgactcgacg agagggccag gatggccatg acctccgagg cttcagtgat
     6841 cacaaagctc gccggacgac accgggcgct ctcgtcgagc gagatgtggc ggagcgcccg
     6901 gtcgctcaca ttcaacgatc gtggccagct caggcgagcc ggatcgatgc cgagtgcgtt
     6961 gccatggaac aggtggttgt cgatgaacgc cgacaacaaa ttgtgactgg ctgaaacggc
     7021 gtgagcatcc cccgtcaggt gcaggttaat ctcttccatc gggaagactt gagcgcggcc
     7081 accgccggtt ccgcctcctt tgattccgaa taccggtcca agcgacggtt ggcgcaatgt
     7141 caccacagca cgtgtgccga ctcggcagag tcccatcgcc aatccgatcg aagtcgtcgt
     7201 tttcccttct cccaacggcg tcggattgat cgccgtcacc aaaatgtatt ttccctccgg
     7261 cttgcctttc agccgatcca gcgcctggag agccaccttc cccttgtatc gtccataggg
     7321 atgcagttca tccggggaga gaccaagtcg tttggcaatg tcctggatag ggagaggtcg
     7381 gtacgctctc gcgatctcaa gatcggtcat ggggttggtg agatggaagg tgcggccagt
     7441 gtcgtgtata ccacggcatc cctgcgcaga ccggacaaaa aaatacgcgc tatgtcagac
     7501 gactagcgcg taatcaggct cgctggaaga accatttagt cgagaatgta tttcgaagga
     7561 tcaaatgtct ggccgttcac cttgaccatg taatgaagat gcgggccagt ggcgaggccg
     7621 ctgcttccta ccaatcccac cacatcgccg cgcttcacac gctgcccttc cttcacaaga
     7681 gccttggcca gatgtccgta gagcgtctcg ataccaaatc catgatcgag tttgaccaca
     7741 tttcccagct tcgaatcaaa ccctacattc gtgacacgac cctgtgcggg cgcctgcact
     7801 ggcgcgttgg cggcagcccc gatgtccagc ccatcgtgcc acgccggctt ttctgtaaat
     7861 ggggacacgc gcggtccgaa tcctgaagtc acccagcctt tgaccggcca aatggaaggg
     7921 gtggcagccc agcgggagga tttctgttcg gcagcgagcg cgagttcctg aagggtatgt
     7981 tcttgcacat cggcttcttt ggcaagccag acaatacctt ccctgacagc agccacgagc
     8041 tcttgttcgt caagatccgt tgactttgag gtgttcagct caattaggcc atcgacggca
     8101 ttcggttgct gaggcacggc accactgcga atgagcttgt ccggggagtt cagcgtactg
     8161 ccctcgggaa gcggggtttc ttcaccaccg cgcccgttta actggtcacc cgtctttggc
     8221 atttcaatac ccagcatgac tcgaaggcgt tggttgacct ccttcatgct ggtcagacgc
     8281 tttttcaaat catcgactgc tgtggagaag gccgcagtct gttgacgagc gctgagcgct
     8341 tcgctacgga atgccgacaa ctcccagact tcgcctgtcc taaccacgta gtgggagatc
     8401 actagaacat cggccgcaag gagaattgca gctaaaatca atatcttacg gacgaatttc
     8461 cttggaaagc taaaccggag tggcttggcg gtggaaccac ggaagattac caccgtatag
     8521 gaatcgttgg actcaacgcc gctgacttgt cccatatccc tcaccccctg ctgctaaggg
     8581 tgcccctccg catgcccaat cataattgcg agtgtaccca tctaggcagc gttggtcaac
     8641 ccctcaattg gtcaagaacc acgcatccaa atctcaatca ctagcggttt cccgatggac
     8701 ccacgctata tatcgaggta acccttttgt cactttcaga gcaatgattt ctggaacctc
     8761 gtatggatgc atcgagccta ttgtttttgc cagggcagcg tacctgcggc cagtcgtttt
     8821 caggatcagc agtgcctccc ggcttttctc aatgcggccc ttccatctaa aaatcgaggt
     8881 taccggcgaa aggacgttcg cacaggctgc cagcttcttc ttcacaactg cttgccctat
     8941 acgtaccgct tctcgccggc tgggcgtcgt gatcaagatg acgagaatct gagcgtccgt
     9001 gttcctcatg ggccgacctc cctaaaatcc caattggcgg caacggccac gaggaccttc
     9061 agggccattg cctgagcaaa taccgtgccc tacctagaag caaaaaagat acatgactaa
     9121 atatttcaat gccttaggtc gtgttattgg acttcatgct ctaggatagc caaggaaaga
     9181 ctcgtaggaa gcgactgatg gtgcagtatt acgtaacaaa aatgaacaat tattgtcctc
     9241 aggtgaactg cgtaccacat cccagtcgaa cagcacagcg ggcagcaaca tgactgggtc
     9301 gggccgtgtt gagtggactg gattgacagc cttttcgagg ctcacttatg atgcctcaaa
     9361 gaggaaaaac cggaggcgat catgggttac accatcactc ccactgaatt gaaaagccgg
     9421 ctcgataaag gcgaacagct cgtcctcttg gacgtccgag aacaatggga attcgatctg
     9481 gccaagctga acggttcgac gctcatcccc ttggcaacgc ttccccaatc gctgggtaaa
     9541 ctcaaccgcg aaaccgagat cgttgcgatt tgccatcatg gaatgagaag cgccgacgcg
     9601 accaatttcc tcctccaaca aggattttcc aacgtgaaga acctggtggg agggatcgat
     9661 gcttggtcag cgcaagtaga tcgatcagtt ccgagatatt aatgtccgcc tgcgtgacgc
     9721 agtgacgcgc catccgtcag tgtgcgcgag actggaagta ctcaaccgtc ttcttcaggc
     9781 cttcgaccag atcggctttc ggctcccacg attgttcttg acgcagtttg gtgttgtcga
     9841 tgacactgcg cgcctgctca cccttcttgg caggaccgtg aatctccttg caaaccgagc
     9901 ccgtatgctg gactaagatc ctaaacagat cgttgatgga cgtttcgacg cccgtaccta
     9961 cgttatacgt gccctgcgtt tcctgaccga tcatggccaa attggcttca acgacgtcat
    10021 cgacgaagac aaaatcgcgc gtttgtcgtc cgttcccatt gatgacaggt tgctcattgt
    10081 tcagcatctt ctgaatgaag atcgccacga cccctgcttc tccctccgga tcctgccgcg
    10141 gtccatacac attggcatac cgaaggctga cggcttgaag cccactgacc cgttggaagt
    10201 acgagaggta ctgctcgccg cacagtttgc tcaagccgta aggagacaac ggcttcagca
    10261 cgtgagtttc gggagcagga tacgtttcct gttcgccgta aatcgctcct cccgaagacg
    10321 aaaaaatgac tttccgtaca ccgtgtttga cggcttgctg caggacattc aacgttccga
    10381 ggatattgac ctgcgcgtcg aacatgggat cttccaccga cttgcgcaca tccatttgtg
    10441 ccgctaagtg catgacgacg ttcggccgtt cgttgcgaaa gacccgttcc agccgccagc
    10501 tttgaatgtc catcttgtaa aaacgggccg cacgattgat gtttctgcgt tttcccgtta
    10561 tcagattgtc gacaacgacc gcctcatgtc cctccatgac aagccggtcg accagatgcg
    10621 acccgataaa tcccgcaccg cccgttacca acaccttcat gcttcctccc gtccttcgcg
    10681 atcaccgcct atgaagacgc gattataccc cgacgcagaa agctgacaag gatttcccga
    10741 ttcccttttc ggcctttaac cggagagtcc aatgtaccgg tactgtggag tccaagccga
    10801 gcggcgcatt caatcacccg ctccgtcacg gcgcggcgct gcccgtcatc tcgtacgatg
    10861 cccccagctc caacctggcc tttgccgact tcgaattgcg gcttgatgag cgcgataacg
    10921 cgggcacctt cacgcaacaa aggcacgaca ctcggcaaca ccaccgtaag cgagataaag
    10981 gatacgtcga tgacggcaag gtcaattggt tctggtatga gcgaccggtc cacataccgg
    11041 atattcgtgc gctcaagtaa aatgacgcgg gagtcctgac gcaacttcca gtcgataagc
    11101 ccatacccga catccaccgc atagactcgt tttataccgc gttggagaag acaatcggtg
    11161 aatccaccgg tggagcagcc gacgtctagt ccgactaggt gccgcgggtc ggccgagaag
    11221 gcatcgagac ccgccgcaag tttctcgccg gaccggctga caaagggagc agctcgttcg
    11281 atttcaatgc gcgcgccacg cggcacgagc cttgcgggct tatcgactac cgtgccatcg
    11341 accagcaccc ctccagcgag gatggtcctg acagccacct cacggctgct gaccaggcca
    11401 tggcttacca gcagacgatc caaccgctct tttccaacgg gcgctttcga attcatcaga
    11461 tatgaagagg gaatcgaaac agaaccagga gggcacacga ctgaaaggac gtatcgcgaa
    11521 atcaacaaga cgactcgtcg gcggattctt cggcttcttc gtcgggtgag aacgcatgga
    11581 gtcgtttctt cccgtttcta tcctgaacaa gcacttccac tttgcgttcg gcatcctcca
    11641 agatctttag gcagctcttg gagagacgaa tgccctcctc gaaaatcttg agggattcat
    11701 ccaggggaag atcccctttt tctaattcgc cgacaatggc ttccaatcgc gccatcgcct
    11761 gttcgaactt cactccgccc acaatgatac cgtcctctca ccttgtgaag gcccattata
    11821 ccgagcccac cagtggattt acaccagtgg atcgggcacc acgcgcttga ccgtacagtc
    11881 gagctcaccc tgtgccaagc gcgccacgag ttcttcgtcc acttccacat ccagcgcgct
    11941 gcgaagaatc gtgccatcgc gggcgcgcat gagtatgctg tatccgcgac ccaaaatcgc
    12001 cagcgggctc aggtgtgtca actgcgccgc tatcgcagcg atccgtcgtt ttcgtgtttc
    12061 gttcaacacg aggatctggc gttccagacg gtggaccagt gttggaatga gcgagaggcc
    12121 acgcttcaca taaaacatcg gattgaggcc ggctagttct cgttgatgtt gacggaccag
    12181 atcccgactc accgccaccc gctcgcatac aagcgttttc agacggccga tggttccgtc
    12241 ggtccgttgc cactctcgcc ggatgcgtag ctggacttgc gcgaggccgt tgatggaggc
    12301 gtccaaccgc cgctgctcca gcacacaata cctggccatc gcatggccgt tgcgcaccgt
    12361 caaagcccgc aaccgctcaa ccacgtcagc caaaacgggc acgacagtct ccgcagctgc
    12421 agaaggggtc gccgcccggc ggtcggcggc gaaatcggtc agcgtcacat ctgtctcgtg
    12481 tcctaccgca gagacgatcg ggatacgaga ggcagccacg gcgcggacaa cgacctcttc
    12541 gttgaaactc cagagatcct ccatggaacc tccaccgcga cccacaataa tcacatccgc
    12601 cacagcctgt tcattcaaca atgccaccgc atccgcgaca tgttgaccag cgccttctcc
    12661 ctggacaggc accggaacca gaatgatatg gagaatcggc caccgccgat ggaggaccga
    12721 gagcatgtcc cgaatggccg ccccggtcgg tgaagtcacc accccaatcg aggacggaaa
    12781 tgcgggcaag ggtttcttcc tggcttcatc gaacaggcct tcagcagcaa gtcgcgcctt
    12841 gagttgctcg aacgccaact gcaatgcgcc gatgcctttc ggctcaaccg tttccagaac
    12901 gatttgatac tctccccgtg gctcgtagac ggtcaaccta ccacggacga tgatgcacat
    12961 gccttcttga agcgtaaact tgaggcgtaa ggcgcttgat cgaaacagca ccgctcggat
    13021 ctgactggat tcgtccttga gcgtgcaata gatgtgacca gaacctggca tgcgaaggtt
    13081 cgacacctct ccttcgagcc atacgtccga gaattgacct tcgacggtgg tgcggagtaa
    13141 gccggtgagt tgggaaacgg tcagcaccga ctttgagggg tggaataaag tctgttgcct
    13201 tgtggccgac atcctagtca gtgaaccgga tccgttcgat tgacagcgcc ttgccgattc
    13261 gagcatccaa ctcgatcaag gcagcgcaaa atacggtggg gccgcccgca acctcgaagc
    13321 gccggggcat gcccgtcaga aatttttcga tggcaagctc tttcttcaca ccaatcacgc
    13381 catgcagagg ccctgtcatg ccgatgtcgg tgagatacgc ggtgcctctg gggagaatct
    13441 gctcgtctgc cgtctgcacg tgggtgtggg ttccgaccac cgccgtcgcc tccccgtcga
    13501 gataatggcc catcgccatt ttttccgagg tggcttccgc atgcatgtca acgagcactg
    13561 cagcgaccag tttcctcaat cgcgacatct cgcgcttagc gacttggaac ggacaatcga
    13621 gcgtaggcat atacgcgcgc cccatcaact gtaagacagc gacttcttca ccgttcttgg
    13681 ttcgaatcac gacgctgccc tgccctggta cgccttcggg atagttggcc ggtcgaagca
    13741 gccgccgctc gcgcgggaaa taatcgagaa tctccttttt atcccaggcg tgattgccgg
    13801 tcgtgatggc agacaggccc atcccgaaca gttcatcggc caggtcaggc gtgatcccga
    13861 acccgccggc cacgttctcg ccgttaccaa cgaccacatc aatctgacgc tgggatacca
    13921 gacgcggaac ggcccgtgcc actgcacgcc gtccaggctc tcccatgatg tcgccgatat
    13981 acaagacttt catttggcgt actcgatgta ccggctctcg cggatgacgg tcactttgat
    14041 ctgaccggga taagtcaact cttgttcgat tttcttggcc aattcccttg aaagctgaaa
    14101 ggactcactg tcagtcacgt cctcctgccg gacaatcacc cggatctctc gtcccgcctg
    14161 gatggcgtac gccttttgga cgcccttctg ggcgtgagca agcgattcca acttttccag
    14221 ccgcttgacg taggattcga gcgcctcacg ccttgccccc gggcgagcgg ccgacagcgc
    14281 ctcagcggcg gccaccaaca ccgattccgg acagatcggc tcgacctgtt cgtgatgcgc
    14341 agcaatggca ttgacgatct tttcactctc gccatatttc tttgctatct ccgccccgag
    14401 catggcgtgg ggcccctctt cctcgtggct taccgctttt ccgatgtcgt gcaagagcgc
    14461 acctcgcctg gccagccgta cgtccaagcc aagctcggag gccatgatgc cacaaatata
    14521 ggaagcttcc cgagcatggt agaggttgtt ttgtccgtag ctggtccggt acttcaaccg
    14581 gcccaggact ttgatcagct cgggatgaaa atccgaaagg cccaactcga agatgatctt
    14641 ctcggcttct tcgtacatca gcttgtcgat atcgactttc accttttcga cgatttcttc
    14701 gatccgcgtc ggatggatgc gtccatcatg catcaaccgc tcaagcgaga ccttggctat
    14761 ttcccgccgc aaaggatcga atcctgagat gatgaccgcc tcgggcgtct catcgatgat
    14821 cagatcgatt ccggtcgccg cctcgatggc acgaatgttg cggccttccc ggccgatgat
    14881 gcgtcccttc atggcgtcgt taggaatggg cacgactgaa atcgtggact cagagacata
    14941 gtcccgcacc acgcgctgaa tcgaagtcgt gatgatttcg cgcgcttccc tttcggcatt
    15001 ctccctggct tcttccaatg tccgtttggc aatgccggcc gcatcgagac gcgcctgact
    15061 ttccatttcc tgcagcagat gtctcttggc ttcgtcggcg gtcatgccgg ccacacgctc
    15121 aagcgcctcg cgatgctctc tttcggcctg ggcgcaggcg gcttcctttg cgataagttt
    15181 ctcttctttc cttatcaatt cctggtcacg tttttggcag tctgcgtctc gcttgtccag
    15241 cacgctgatg cgcttatcaa tggcttcttc acgttgcacc acgcgcttct cggttattga
    15301 aagttccagc agcttggcct tctgttcctt ttccaattcc gcttttgact gaaacaccaa
    15361 atccttggct tcgagtttgg cttccttgac gatgttttca gcttcgcgtt gggcgttttg
    15421 gatcgtttga cgggcctggt cttcttcctg agcccgtttt gcggtcgccg atgaacggcg
    15481 cgccagttca aacagaccag ccccgagtaa cgctcctacc aatccgacga gtatgtacat
    15541 cacaatggag gtcgacatag gacggcccct ttcacgattg aggcgccagg cagtctcgcc
    15601 tcacacaagc tatagtcgag aagggtatag ggaaggcagg aaggagacga ttgaaacgaa
    15661 agacactaga ccggaccggc taccccgagt accgactccg caaggatgaa cgtggtgttg
    15721 gcagaacgga acgcgcctgt ttccatccgg acacactccc gtccgctctg ggataacggc
    15781 tcactcgtgt gaacctcgag cgcaccaggc gggggggatc tgaaacggaa accagcgccg
    15841 gcctactccg gctcaaagcc ggttcggtcg gcagcacgtg ctccactagc ctgtccagta
    15901 tggcaggcag ggctgataga agaagcacga acccttgaaa aacgctccac tctctcaacg
    15961 gcaccagcat cgaaaaaccc acatccatct ttccagtgac gcaatcggat cccggatttc
    16021 ttaccggttg tagttacctc gtcctcatcc caatcaataa acctgcttcc gcaaatacca
    16081 ctaccacggc accacgatag caaaggcctt ccaggctttg caagcgcatt caacgaaaca
    16141 gcgaggtggg cacctgctct tcgattgact ccataagaga cgtcattcgg cgttcgacat
    16201 ccgcttcgcc ctgagcacgc ttcttttccg cttccaacag ttgatgggcc agattgagag
    16261 ccgtgaggac cgccagcctc gacggagtcg tcgtattcat gccttctgcg agctgccgca
    16321 tttgcccgtc cacaaaccct gccagccggc ggatatatgt ctcgtccgcc tctcccctga
    16381 tcgaataacg ctggccatag atttcgacct ccgtcgtctt agtcaaaggc cacctccttg
    16441 cgttcttcaa atgcctccaa cagctcgata tctccgagca ctttttcgac acgggcttta
    16501 atgtccgaac gttctcgcgc ccaccggcga ttggaatcac tttcgtccgc caatcgctgc
    16561 cgagtcgatc gcaaatcgtc ctcgaggcca gcatttcgct ttttcagctc ttgaatcaac
    16621 ttgaccaggt ctttgatgcg tgtttcaagc gcatccaaac gatccaacgc catatgcgtg
    16681 tacctacttt gtagccacta ccgattgtgg cgcactatag gaatttaccc agggcttgtc
    16741 aacgccttca ggacagcccg ttgagccgta cgtactcgcg gtaactgcgc aacacccgtt
    16801 ttacatactg tctggtttcc tgatacggaa tcagctcgac gaactcgtcc tggttgtgac
    16861 cgcggtactg agcaatccag ttgccgaccg ccaatggacc cgcattatac gaggccacgg
    16921 tgtagaccag gtttcctgaa aactgttcca gcaattgctc gacatagcga acgccgatac
    16981 gaatattggt ttcttgatca aacaggtctt cacgtccgag ggcgggaagg cccacccgct
    17041 gcgcaacgtt gttggctgtg gccggcataa cttgcattag accgatggcg ccgactcgcg
    17101 aaaccgcttt cacatcgtac tggctttctt cccggataat ggcggcaatc agataggggt
    17161 cgacgccttt ggcgccctgc agtttaatgg tgggcagcag gccggtggga taggccacgt
    17221 tccagagtga gggggtaaac gccccgccag tccgctccaa ttgatcgcgg aatctggcgc
    17281 gggcgagccg cagggcctgg tgataagcac cgacctcgtt caacatggtg gagagcgcga
    17341 tcaagacatc cggatcccgg ctataccgct ccgttaggac ggcgacttca tgggcggcat
    17401 ccgcatccaa tcccaaggtc ttgagttcaa tcgcccgacg gtaggccgtg tgctgttcga
    17461 tgtctgcccg cgtcaccgtg ggtcgggccg gatcaccagt cttggcggtg tcggaagaaa
    17521 gcaccatcgc ctcggtaggc gcatcagcaa cgggagggat cttcatccgt tctctcgcca
    17581 actggcaata gtaggtgaac gggtggcgct gacagagctt gcggtagaaa tcctgccctt
    17641 cgagcggctg attgttcatt tccccggccc gtcctatcca atagagcgct tggggttcga
    17701 attcactgtc atgttgatcg gcgagcgccc gtaagctctc tcccgcctcg cgatatcggc
    17761 cagttcgata atagacccat ccgacccgcc attggccctc ggcccgctgt gcggcgggct
    17821 ctccatgctg tgcgacatgc acatatttac caatcgcttc gtcgaattgc ttctgatctt
    17881 ccagccacat accggcaaac aacgtgatct ggcccttctg ttcgggggag agggaggaag
    17941 aggccagggc gcgggccgct tccaaaagct tttcgccttg tccttggcgc aaatagacgc
    18001 gcgccagcca caccgctgct tcgcgcgact ctggtcctcc atctttcgtc aagccgcgga
    18061 acgtttcacg cgcttgatca tagagcttga gtcgcacctg tgcgattcct agcttcaact
    18121 tggcctcggc tcgtcgaggc gagtgcggat cggccaggag atattttttc agctcttcaa
    18181 tcgcctcggc gtgataggcc tgtcccaaga aggcctgggc tctgttgagc cgatcggcgg
    18241 gttgcacggt ccacgtttca ccgccaagat tcgacgtcaa taaggtttcc gcctccttgg
    18301 cttcacgcgt atagggaaac cgtacccata cctgctgcag cagctcgcgc ccttccacga
    18361 cgttgctttc ccgtaggcgg cacgcgccac gccgcaggta ggcctgggat gcgccggtat
    18421 ccttatcgtt catcgcgacg gccctgccga accaggtgat cgactccgcg caagcggccg
    18481 cctgatacca cgcttcaccc gtcttgtacg ccgccctgcc cagaatgttg gaatcgggca
    18541 ctgccatggg gatgctttcc aacaacaccg ccgcctctct tgcttgtcct agattcaact
    18601 gtgattcagc gatccacaga cggatgtagt cgtccaggac ggaaaaatcc cgctgcgcag
    18661 tgcggagaaa ttgcatggcc gaagcggggt cgcgctcgat caacaacacg cccgacagga
    18721 gccccgcccg ttttccccac aaggtgcccg aataccgatc cgccaagtgc tgaagccgtt
    18781 cgagcttcag cgtgagcacc tgctccttgt tcatggtttt cccaagccgt tctttgggga
    18841 atgccgctga ttgaaaacac agctcaggct gctcgcaggg atccgcttcg cctggaaggg
    18901 cagccgtgcc gtccgctccg ttaatcagga gcaggaggcc gcaaagacag aacctgacca
    18961 ggacagcaac cacgaccaac ctcatcatga agcttgatgg ccctatcata tataacagtg
    19021 tccttcgatc catgccagaa gaagacatcc tgcggattcc ttgttccact ttttctgcgt
    19081 gtgttaggct gcgccatgcg tggcgtgaag acgatggctc ccctgacgac gatcaatcta
    19141 cttactctcg acgagtccga gacggctgcg ctcgttcgga gtctcggctg gccttcctat
    19201 cgcaacggcc agattcttcg ttggctctac caacggcggg tacggtcgat tagccaaatg
    19261 accgacctca gtcaagcaga ccgggccaga ctgggaagcg aagccaggat cggacgagcg
    19321 cttggatgca cggtgtttca atcagcagat ggcaccagaa aactgatctt gaagttggaa
    19381 gacgacctcg aggtggaagc cgttctgata cccgacgacg agcggctgac gctctgcgtg
    19441 tccacgcagg ttggctgcac attggactgc ggcttctgcc taacgggcac aatggggttg
    19501 aagcgcaatc tgaaggcgca tgaaatcatt gaacaggtgt tgacggccca cgatcacctg
    19561 aatccggggg aacgcctctc gaacatggtg ttcatgggaa tgggtgaacc gttggcaaat
    19621 ctcgccgcgt tgtctgaggc ggttcgccgg ctgaccaaca cgaaatgggg actcggctgg
    19681 tcgccgcggc ggatcaccgt ctcgaccgcc ggattggcct caaaactaca cgaagtggcc
    19741 ccgcttggcg tgaatctggc gatttcgttg aacgccacga ccgaagagca gcgggctcgg
    19801 cttatgccgg cggttcatgg catcgcctca ctcaaaacgc tgctctccgc atgccgtcgc
    19861 tttccgttgg ctcgccaacg acggttgacc ttcgagtacg tgctgctggc gggtgtgaac
    19921 gaccgagaac tcgatgcgaa gcggctagtt gccttgatca agggcatcaa ctgcaaggtc
    19981 aacctcattc cattcaatcc gtttcccggc agtccgtttc gtcgtccctc cgaccgagac
    20041 atcctcacgt ttcaatcgat tgtgcgacag gccggcgtgg acgtgttcat tcggaaaagc
    20101 cggggccggg acgttctggg tgcatgcggc caactgggaa atctccctcc gtc
//
LOCUS       DASTQY010000245         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1097001, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000245 DASTQY010000000
VERSION     DASTQY010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097001"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..119
                     /locus_tag="VFR82_08180"
     CDS             <1..119
                     /locus_tag="VFR82_08180"
                     /inference="COORDINATES: protein motif:HMM:NF017024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEU4501615.1"
                     /translation="THRASSRDTFARGALRAARWVVKQPPGLYDMMDVLNLK"
     gene            complement(895..1209)
                     /locus_tag="VFR82_08185"
     CDS             complement(895..1209)
                     /locus_tag="VFR82_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501616.1"
                     /translation="MTRRWLLALIGCLLLSACGGNNPYLEQSLKPAELQGKDKSWFVK
                     NWGEPSGKAPRFFGGETWTYFRIAGGKSGPPLFNFSPNQCEITLKFDKEEKLSDYSYS
                     GC"
     gene            1429..2076
                     /gene="fsa"
                     /locus_tag="VFR82_08190"
     CDS             1429..2076
                     /gene="fsa"
                     /locus_tag="VFR82_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU4501617.1"
                     /translation="MKFYLDTASVKEIQEAASLGLLDGVTTNPSLVAKEGRVFREVLL
                     EICNIVDGPISAEVVSIEADAMVKEGRELAKIHKNIVVKVPLIAEGLKATKRLTAEGI
                     RVNVTLCFSPTQALLAAKAGAWCVSPFIGRLDDISSSGMELIRQIVTIYKNYEYKTYI
                     LVASVRHPQHVVEAALAGGHICTMPFSIFQQMVKHPLTDIGLKKFLADWDAQSKK"
     gene            complement(2139..2843)
                     /locus_tag="VFR82_08195"
     CDS             complement(2139..2843)
                     /locus_tag="VFR82_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU4501618.1"
                     /translation="MLHFGAMRSLTVLNQHIITCTACPRLVTYRQAVAQEKRRQYRDW
                     TYWGRPVPGFGDPAARLYVLGLAPAAHGGNRTGRIFTGDRSGDWLYEALHHFGFANQA
                     TSTHKDDGLALSDCYIGATVRCAPPDNKPMPEEFERCRPFVREELRLLQKMRVVVVLG
                     KIAFDHYLKACREEGRMLPAPLPTFGHGTVYRLPWHVTLVGSYHPSQQNTFTGKLTKP
                     MFHAVFTMARSAIERG"
BASE COUNT          681 a          831 c          886 g          662 t
ORIGIN      
        1 tcacacaccg ggcgagcagc cgagacacct ttgcccgcgg cgcactgcgt gcggcccgtt
       61 gggtagtgaa gcaacccccc ggcctctatg acatgatgga tgtgttgaat ctcaaatgac
      121 aagtgagcgg ataggctcgc gagacaggtg ttaatttaaa cgacgaggga aaagaggaga
      181 ggcgaaacag agcgaccagt cacaagccgt agcacgaccc cggagactca tgctcgtgag
      241 agcgggtcga acgatcgttc atcgtgtcaa tgtctagccg taagaggacg aggttcttga
      301 tccgctgcct tccgacgacc acagtttaag caggcaaata ccatgatcgc aagcccggca
      361 tccaaatcca ccgcccgttc cggcaacatt gttccgcgac acttgtcaca ggtcttcatg
      421 cggtcactct aacactgcga ccgaagggtg cctatccatc agaagtactg gggtcgtgag
      481 ggaattaagg cctggtcgtc ggggtagaag ctaggccatt ggagctatag gagttcgaac
      541 tgaccgttcg tggacgttca catcggccgt tgacacactt tcgacgctcc cataggattg
      601 tggcagccga ctcgtacctc tactatgtat agaatcatct tcattcttat cctgctgatc
      661 atcttgtact tcctgctccg aagcgccttc cgggagtttg ggactcgaac acaggaacgt
      721 ctgccgaaac ccaaagatca gatggtgcag gatcccgtat gccgtgtatt tgttcccaga
      781 gggtcggcgg tgaccgaagt cattggtggt cagacttatt gtttttgcag ccagggctgc
      841 gcgacaacct ttcaaaggca gatgaccggg taaccgggca tggtgtggaa ggggtcagca
      901 gccggaataa ctatagtccg acagtttttc ttctttgtcg aacttcaagg taatctcgca
      961 ttgattaggt gagaaattga aaagtggcgg ccccgatttt cccccggcga tgcggaagta
     1021 ggtccatgtt tcgccgccga agaagcgagg ggccttgccg cttggctccc cccaattctt
     1081 gacgaaccag gatttgtcct tcccctgcag ctccgctggt tttaaggatt gttcaaggta
     1141 gggattattt ccgccgcagg cagacagcaa caggcatccg atcagcgcca acagccatcg
     1201 tcgagtcatg gaagtctctc ctcgcagtga tcagcactgc atctttctac cagaccggcg
     1261 cgggagggat tctaaccgcg ggtcatgggg gtgtcaaaca ggaatggcct tggcgccaac
     1321 gcggcgtctc ggcgccgcgg ggtgggggtg agacctagcc tgtttgcatc accaggtcat
     1381 tctttttaca atgtgggtat gagtgcgcag acatgaaagg acacggctat gaagttctat
     1441 cttgatacgg ccagtgtaaa agaaattcag gaagcggcaa gcctgggctt gctggacggt
     1501 gtcaccacca atccttctct cgtcgcgaag gaaggacgtg tgtttcgcga ggtgcttctc
     1561 gaaatctgta atatcgtgga cggcccgatt agcgccgaag tggtcagcat cgaggccgac
     1621 gccatggtca aggaagggcg tgaattggcc aaaattcaca agaatatcgt ggtcaaagtg
     1681 ccgttgattg ccgaagggct taaggcgacg aaacgcctga ctgccgaagg aatcagggtg
     1741 aacgtcaccc tctgcttttc gccgacacaa gcgttgctgg cagccaaggc aggcgcctgg
     1801 tgtgtctcac cgttcatcgg tcggctcgat gacatcagct ccagcggcat ggagctgatc
     1861 cgccaaattg tcacgatcta taaaaactat gagtacaaaa cctatatcct ggttgcgagc
     1921 gtacggcatc cccagcacgt ggtcgaagct gcattagccg gcgggcatat ttgcacgatg
     1981 ccattttcaa tcttccagca gatggtaaag cacccgttga ccgatatcgg cttgaagaag
     2041 tttctcgcgg attgggacgc gcagagcaag aagtaaaggg gtggttaggc ctgctctatt
     2101 ctcacccgcc cgacccggcg cacgccgaga cgtgcgcttt acccacgctc gatggcgctt
     2161 ctggccatcg tgaataccgc gtggaacatc ggcttcgtca gtttcccggt gaaggtattc
     2221 tgttgactgg gatgatagga gccaaccagc gtcacgtgcc agggaaggcg gtagaccgtt
     2281 ccatgaccga acgtgggaag gggcgcagga agcatgcgcc cctcctcgcg gcacgctttc
     2341 agatagtgat caaaggcgat tttccccagg accacgacca cccgcatttt ttgcaagagt
     2401 cgtaactcct ccctcacaaa cggacggcac cgctcgaact cttccggcat aggcttattg
     2461 tccggcgggg cgcagcgaac ggtggcgccg atgtaacaat ccgacaatgc aaggccgtcg
     2521 tctttgtgcg tcgaggtcgc ctggttcgca aatccgaagt gatggagcgc ctcgtaaagc
     2581 cagtctccgc ttctatcgcc ggtaaagatt ctccccgttc ggttgccgcc atgggccgcc
     2641 ggcgccagtc ccaaaacata cagacgcgcc gccggatcgc cgaatccagg aacgggtcgt
     2701 ccccagtagg tccagtctcg atactgccgt cgcttttctt gggcaacggc ttgccgataa
     2761 gtcaccagac gagggcaggc agtgcaggtg atgatgtgct gattgaggac ggtgagcgat
     2821 cgcatggccc cgaagtgtag cacgttagtg aaagccgatc ctgcttgccg actctgcggc
     2881 ttgctgctag catgcggact gaaagagatc aggtcaaaca actccgagga taggtgcgta
     2941 tggtcgactg gtggttgcgc cgtttactga ccgtcacagt gatcttggcg tccctctttc
     3001 caattcccac aggggcgcag ttggatccct tgaaagacat accggctcaa tccggagcga
//
LOCUS       DASTQY010000246         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1240105, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000246 DASTQY010000000
VERSION     DASTQY010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240105"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2083
                     /locus_tag="VFR82_08200"
     CDS             <1..2083
                     /locus_tag="VFR82_08200"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEU4501619.1"
                     /translation="LASESRTKLPAYFNRIIQDERLFAIGFCDLRNRLLYKTHSYPDA
                     IACEGNETIRAGETALLQLPRGAVHVSAVGIEGNGGPVGRLMLVHDMSWVQRRSTDTK
                     WYLFYLFAIIGAVISSVTVLVAHLSWRGWVTGVRSMLRGEGLIALIKDKHHGPELQPV
                     AKDLRALIHHLEADKRMKDESQVSWKPATLKSTLHDHLAGDEVLIVSNRQPYIHNRKG
                     EAIEVQVPASGVVTALEPIMRACSGVWIAHGNGSADRDVVDARAHVRVPPEAPSFDIR
                     RVWITPEEEAGYYYGFANEGIWPLCHLAHVRPVFRSSDWQQYQAINERFALAVVEEAK
                     TDNPVVLIQDFHLALVPKLVRDHLPHATIITFWHIPWPNPERYAICPWYVEILEGLLG
                     SSILGFHTRFHCSNFINTVDRSLETRIDWDSSTISYGGKLTAVNPYPISIEWPGRMLA
                     SQPSVFECRKRIREIYALPLDHVVGLGIERLDYTKGILERFLAVERLLELEPQWIGRF
                     SFVQIAAPSRSKIEEYAQFRQQVENLAERINARFGREGYQPIQLLIRHHDVRDVMMHY
                     RGADLCVVSSLHDGMNLVAKEFVAARDDEQGVLILSQFTGAATELPDALVVNPYNIDQ
                     CAAAMHLALTMPPTEQRARMRSMRGIVQEFNVYRWAGRMLMDAARMRQRARMERQTSG
                     RNARTTTGGQA"
     gene            2080..>2781
                     /gene="otsB"
                     /locus_tag="VFR82_08205"
     CDS             2080..>2781
                     /gene="otsB"
                     /locus_tag="VFR82_08205"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEU4501620.1"
                     /translation="MIHALSEAGFRALRDAASRPLLYAFDFDGTLAPISSDRHAVTLT
                     LGVAEWLRELAKRVPCAIVSGRALADVSLRTNGIVPHVIGNHGIESPLTSRSTLTEAE
                     RICADWKRELERTLAPMILLGAEIEDKRYSLTVHVRLAQDPADAVRTSLTWLRRLSPE
                     PRLIEGKYSINALPPGLGGKGEAALALMNHLGRTGLFYIGDEETDETVFSLPDGVTMG
                     VRVGRQAESRARFFLH"
BASE COUNT          552 a          820 c          849 g          560 t
ORIGIN      
        1 gctcgcatca gaatccagga cgaagctgcc cgcctatttc aatcggatca ttcaggacga
       61 acgactattt gccatcggat tctgcgacct ccgcaaccgc ctgctctaca agacgcattc
      121 gtacccggac gcgatcgcct gcgaaggtaa tgagacgatc cgcgctggcg aaacggccct
      181 cttgcagtta ccgcggggag ccgtgcatgt gtcggcagtc ggcatcgaag gcaacggcgg
      241 tcccgtcggc cgtttgatgc tggttcatga catgagctgg gtgcagcgtc gaagcacgga
      301 tacgaagtgg tatctcttct acttgtttgc catcatcggc gcggtgatct catcggtcac
      361 cgtgctcgta gcacatctga gttggcgtgg ctgggtcacc ggcgtccgtt cgatgctgcg
      421 tggcgaaggg ctgatcgcac tgatcaaaga caagcaccat ggtcccgagt tgcaaccggt
      481 cgccaaagac ctccgtgcgc tcatccacca tcttgaggca gacaagcgga tgaaagacga
      541 aagccaggtg agctggaagc ccgccacgct gaagtccacg cttcacgacc acttggccgg
      601 cgatgaagtg ctcattgtct cgaaccgcca gccatacatt cacaatcgga agggcgaggc
      661 gatcgaagtc caggttccgg ccagtggagt cgtcacagcc ttggagccga tcatgcgggc
      721 ctgttcaggc gtgtggatcg cccatggaaa cggcagcgcc gatcgtgacg tcgtcgatgc
      781 acgcgctcat gtccgcgtgc cgcccgaggc gccctcgttc gacatccgga gggtctggat
      841 cacgccggag gaagaagcgg ggtattacta tggattcgcg aacgaaggca tctggcctct
      901 ttgtcatctc gcccacgtcc gtcccgtgtt tcgatcgtct gattggcagc agtatcaagc
      961 gatcaatgaa cgattcgcct tggcagtcgt ggaggaggcc aagacggata atccggtcgt
     1021 actgatccag gattttcacc tcgctctggt gccgaaactc gtccgcgacc acttgccgca
     1081 tgcgacgatt atcacgtttt ggcacattcc ctggccgaac cccgaacggt atgcgatctg
     1141 cccctggtat gtcgaaatcc tcgaaggtct gctgggcagc agcatcttgg gattccacac
     1201 ccgcttccat tgcagtaact tcatcaacac ggtggatcgg tctctggaaa cgaggatcga
     1261 ctgggacagc tcgacgattt cctatggggg taaactcacg gccgtcaatc cgtatccgat
     1321 ctcgatcgaa tggcctggcc gcatgctcgc ttcccaacca tcggtcttcg agtgccgcaa
     1381 gcggatcagg gagatctatg cactgcccct cgatcatgtc gtgggattgg gcatcgaacg
     1441 tctcgattat acgaagggca ttctggaacg ctttttggcg gtcgaacggc tgctggaact
     1501 ggagccccag tggatcgggc ggttctcctt cgttcagatc gccgccccca gccgttcgaa
     1561 gatcgaggaa tatgcgcagt tccggcagca ggtcgagaat ctcgccgagc gtatcaacgc
     1621 gcgattcggc cgggaggggt accaaccaat ccagttgctg attcggcatc atgacgtccg
     1681 agatgtcatg atgcattatc gtggggccga cttgtgcgtc gtcagcagcc ttcatgacgg
     1741 gatgaatctg gtggcgaaag aattcgtggc cgctcgcgac gatgagcagg gcgtcctcat
     1801 tctcagccaa ttcaccggtg cggcgacaga attgccggac gcactcgtgg tcaatccgta
     1861 caacatcgac cagtgcgcgg cggccatgca tcttgcactg acgatgcctc cgacggaaca
     1921 acgggcgcgg atgcgaagca tgaggggcat cgtgcaagag ttcaacgtct accggtgggc
     1981 gggccgaatg ctcatggatg ccgcccgcat gcgccagcgg gcccgtatgg agcgacaaac
     2041 cagcgggcgg aatgccagaa ccacgacggg aggccaggcg tgatccatgc cctctcggaa
     2101 gccggttttc gtgcattgcg cgacgccgcg tccagaccgc ttctttatgc cttcgacttc
     2161 gacggcacgc ttgcgccgat ctcaagcgat cgccatgccg ttactctaac gcttggcgtg
     2221 gcagaatggt tgcgtgagtt ggccaagcgg gtgccgtgcg ccatcgtgtc gggaagggcg
     2281 ctcgccgacg tgtcgctgcg gacgaacgga atcgtgcctc atgtgatcgg caaccacggc
     2341 atcgaaagtc cgttgacgtc tcgcagcacg ctgacggaag ccgagcgaat ctgtgccgac
     2401 tggaagcgcg aactcgagcg cacgctcgcg ccgatgatac tgctcggtgc ggagatcgag
     2461 gacaagcgct attcattgac ggttcatgtg cgccttgccc aagacccggc agacgccgta
     2521 aggacttcac tcacctggct ccggcggctg tctccggagc cgagactgat cgaaggcaag
     2581 tattcgatca acgcgttgcc tcccggcctg ggcgggaagg gtgaagccgc gttggccctg
     2641 atgaaccatc tcggccgaac cggactcttt tatattggag atgaggaaac ggatgaaacc
     2701 gtgttctcgt tgccggacgg ggtcacgatg ggcgtccgtg tcggcagaca ggccgagtcc
     2761 cgcgcgcgct ttttcctcca c
//
LOCUS       DASTQY010000247         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1584440, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000247 DASTQY010000000
VERSION     DASTQY010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584440"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..752)
                     /locus_tag="VFR82_08210"
     CDS             complement(12..752)
                     /locus_tag="VFR82_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEU4501621.1"
                     /translation="MIGAMVFDFNGVIVDDETPHLLCFQQALAEAGLTLTTQDYYGAY
                     LGMDERTCTRLLLEQRDGMCDEARILRITDRKADLFRAHSAHHRPPLFAGAADFVLAA
                     KGCYRLAIASGGRRAQIEDALAGSAIEQAFEVMITAEDCPIGKPDPAIYLLACDRLNA
                     NRGSAERITPSQCLVIEDSLAGIRSARAAGMRVLAVSTTYPLEMLNEAELVFPGLHDM
                     TPQTAIDALALASSRCQPAAFSTPSKDE"
     gene            931..>2523
                     /locus_tag="VFR82_08215"
     CDS             931..>2523
                     /locus_tag="VFR82_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU4501622.1"
                     /translation="MTDYGVLGNLLVIFSVSIAVVFMFHQFRLPSIAGFLVAGALIGP
                     HGLNLIADIGTVQVLAEIGVVLLLFTIGIEFSLVQLASLRRLLFIAGPIQVGTVLAVA
                     WVAAMSVGMTWQQGIFWGFLLSLSSTAIVLKTLAERGDSDSIHGRTTIGILIFQDLAV
                     VPMMLLMPILATRAEGSLTTILFTLAESVFVVGLIIAAAWYAVPKLLEHIVRSRSREL
                     FLLTVIVLCLGIAWLTSLGGLSLALGAFIAGLVISESEYSHQAMADVLPFRDSFNSLF
                     FVSIGILMDWRVLIDHPVLVFGLLLAILFVKFLAGAAASFALKIPPRSAIMVGVALAQ
                     VGEFSFLLAQQAQESGLLRGDPYQVFLSVSVLSMIVTPFLMQWSPAMARRAEAIQRLR
                     RWLPTRTEAHVLQTEGSHIRLKDHVIIVGYGLNGRNLARVLSETEIPHLALDLDGDTV
                     RRESRHGVPIYYGDATNPNVLRHVKIEEAKVLVVAISDPFITRRTVQVAKVLNPKIRV
                     VVRTRYLRELEELHDIGADDVVP"
BASE COUNT          448 a          726 c          759 g          590 t
ORIGIN      
        1 gaggaatgcg tctattcatc tttggagggg gtcgaaaagg ctgccggttg gcatctggag
       61 ctggcgagcg ccaacgcatc gatggctgtc tggggcgtca tgtcgtggag gcctgggaac
      121 accaattcgg cttcatttag catttcaagg ggataggttg tgctcaccgc caacacgcgc
      181 atgccggctg ctctggccga tcggatgccc gcaagcgaat cttcgatgac cagacactga
      241 gaaggggtga tcctctcggc ggaaccgcga ttggcgttga gccgatcgca ggcgaggaga
      301 tagatcgccg ggtctggctt tccgatcgga caatcctcgg ccgtgatcat cacctcaaat
      361 gcctgttcga tcgcactacc ggccaacgcg tcctcgatct gcgcccgacg tccgcccgag
      421 gcgatcgcca atcggtagca gccttttgcc gcaaggacaa aatccgctgc gcccgcgaac
      481 agcggtggtc tgtgatgggc actgtgcgcc cgaaagaggt ccgctttccg gtcggtgata
      541 cggaggattc gggcttcatc gcacatgccg tcgcgctgtt cgagcagcag ccttgtgcag
      601 gtgcgctcgt ccattcccaa atacgctcca tagtagtctt gtgtggtcaa tgtcaggccg
      661 gcctcggcca gtgcctgctg aaagcaaagg aggtgaggcg tctcgtcatc gacgatcaca
      721 ccgttgaagt cgaacaccat ggcgccaatc acgccggcac tctacgcgag ccttccaacc
      781 ggcacaagtg gcaagacctt gttcgtgtcg ttgcactggg gtattctccc ccgacaggaa
      841 gcccaaccac ctgaccgtct cattcatcaa gcggcgtgac taggcacgcc attcgccaga
      901 cggcttttct atcctgacgc cactgctgct atgactgatt acggcgtgct cggcaatctc
      961 ttggtcattt tttccgtctc gattgccgtt gtcttcatgt tccatcagtt ccgactgccg
     1021 tccatcgccg gtttcctggt cgccggcgcg ctcatcggcc ctcacggctt gaacctcatc
     1081 gctgacatcg gcacggttca agtgctcgcc gaaatcggcg tcgtccttct cttgttcacg
     1141 atcggcattg aattttctct cgtccagctc gcgtcactgc gccggctgct gttcattgcc
     1201 ggacccattc aggtgggcac cgtgctggcg gtcgcgtggg tggccgccat gtcggtgggc
     1261 atgacgtggc aacaaggaat tttttgggga tttcttctct cgctcagcag caccgcgatc
     1321 gtgctgaaaa cgttggccga gaggggggac agcgattcga ttcacggtcg cacgaccatc
     1381 gggatcctga tttttcagga tctcgcagtc gtgccgatga tgctgctcat gcccattctt
     1441 gccactcgag ccgaggggtc gctgacgacg atcctgttca cgctggctga gtcggttttc
     1501 gtggtgggat tgatcattgc cgcagcctgg tacgccgttc cgaagctact cgagcacatc
     1561 gtgcgcagca gaagccgcga gctgtttttg ctgacggtca tcgtgctctg tctgggcatc
     1621 gcctggttga cctctctcgg agggttgtct ctcgcgctcg gggcatttat tgccggactc
     1681 gtgatatccg aatcggaata tagtcaccag gccatggccg atgtgctgcc gtttcgcgac
     1741 agtttcaaca gcctattttt tgtgtccatc ggcattctga tggactggcg tgtgttgatc
     1801 gaccacccgg tactggtgtt tggcctgttg ctggcgatcc tcttcgtgaa gttcctcgcc
     1861 ggcgcagcgg cgtctttcgc cctgaagatt ccaccccgat cggcgatcat ggtcggcgtc
     1921 gcgttggcac aggtcggaga attcagtttc ttgttggcgc agcaggcgca ggagagcggg
     1981 ttgttgcgcg gtgatccgta ccaagtgttt ctgtccgtgt ccgttctgtc gatgatcgtg
     2041 acgccctttt tgatgcaatg gtcgccggca atggcgcgtc gggcggaagc cattcagcgg
     2101 ctccggcgat ggctaccgac aaggaccgaa gcgcatgtcc tccaaacgga aggaagccac
     2161 atacgtctaa aggaccatgt gatcattgtc ggctatggct tgaacggccg gaatctggcg
     2221 cgcgttctga gcgaaacgga aattccccac ctggcgctcg atctggatgg tgacactgtc
     2281 cgtcgagagt cccgccacgg ggtgcctata tattatgggg atgcgacgaa tcccaacgtg
     2341 ctacggcatg tcaagatcga agaggccaag gtattggttg tggcaatctc ggatccgttc
     2401 attacgaggc ggacggtaca ggtagccaag gtgctcaatc caaagatccg cgttgtggtc
     2461 aggacgcgct acctgcgcga gttggaagag ctgcacgata tcggcgccga cgatgtggtg
     2521 ccc
//
LOCUS       DASTQY010000248         9918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1232725, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000248 DASTQY010000000
VERSION     DASTQY010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9918
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232725"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /gene="tilS"
                     /locus_tag="VFR82_08220"
     CDS             <1..729
                     /gene="tilS"
                     /locus_tag="VFR82_08220"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU4501623.1"
                     /translation="VDNLYRSTVIVEPGGNQRFVRQAFTRLPAALQRRLVRRSLRAGD
                     PEGRAPNLRIVESVLRFAAGESKGMLSIRGFDVITEREWIVLRLTSQRTASSTSGQDL
                     PAVTVPVSLPATVYWPGTRQQIHVQVMTKQAAASWLNQRRPECAVFDADRLSAPLIIR
                     AWQAGDRIHPRGMRGKRKKLQDLFTDLKVSREERDRIPLIVASEGIVWVVGHRQHERC
                     AVGKNTRRCLVVTVQSKAGSEGAG"
     gene            731..1270
                     /gene="hpt"
                     /locus_tag="VFR82_08225"
     CDS             731..1270
                     /gene="hpt"
                     /locus_tag="VFR82_08225"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU4501624.1"
                     /translation="MERIFGRPIVTQEEMRARIKELGKQIANDYAGKDLVLVGVLKGA
                     YAFYADLARAIRIPMRVDFLIVASYGSQSKTSGKVKVITELTEQIKGKDVLLVEDIVD
                     SGLTVEYLIKTLSKQKPKSIKVCTLLSKPERRTIDVPLDYVGFKIPNKYVVGYGLDYQ
                     QKYRNLPYLAELDMEGEQE"
     gene            1373..3184
                     /gene="ftsH"
                     /locus_tag="VFR82_08230"
     CDS             1373..3184
                     /gene="ftsH"
                     /locus_tag="VFR82_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU4501625.1"
                     /translation="MNSRAKNLLFWVVVGLFMILLFNLFSVPTHAPEEEVIFSDFMAK
                     LDKGEIEKVIIKSSHVSAVLKDKTRIRTFTAEYPDFVKVLREKGVQIEAKPPDESPWY
                     ITFLVTWGPFVLFLGLWFFLMRQMQIGGNKALSFGKSRARMLTEERKKVTFSDVAGID
                     EAKEEVLEIIEFLKDPRKFQKLGGRIPKGVLIVGPPGTGKTLLAKAIAGEAGVPFFSI
                     SGSDFVEMFVGVGASRVRDLFEQGKKHAPCIIFIDEIDAVGRLRGAGLGGGHDEREQT
                     LNQLLVEMDGFDTTEGVILIAATNRPDVLDPALLRPGRFDRQVVVNRPDLRGRSEILK
                     VHTKKVPIATNVELEKIARGTPGFSGADLENLVNEAALWAARQNKKEVEVIDFEMAKD
                     KVLMGAERKSMILSDEEKRTTAYHEAGHALMAKLLPGTDPVHKVTIIPRGRALGVTMQ
                     LPTDDRHNYSKEFLYNNLAILMGGRVAEELVLKHITTGAGNDIERATDLARKMVCEWG
                     MSEKLGPLTFGRKEEEIFLGRELTTKRDFSDQVALEIDLEIKRLVTENYERAKRLLTE
                     HMRSLKALAEALLEKEVLDAPEIDQILMQNSSQTVPA"
     gene            3387..4247
                     /gene="folP"
                     /locus_tag="VFR82_08235"
     CDS             3387..4247
                     /gene="folP"
                     /locus_tag="VFR82_08235"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU4501626.1"
                     /translation="MELNFLERRLSLDRPLLMGIVNVTPDSFFDGGRFDDPRTAVEHG
                     RRLVEEGADLLDIGAESTRPGSVPIDQEEEQRRLLPVVTALAQAVSVPISVDTSKAAV
                     ARAAIKAGAVMVNDVTALRGDPAMVDVVAQTGVALVLMHMQGIPQTMQQAPTYRDVVE
                     EVADFLAERARFAMERGVAQSRIVLDPGIGFGKVLTHNLDLLAHLRMLTTLGFPVLVG
                     PSRKGFIGQLTKQSVEARAWGTAGVVALAVEQGANILRVHDVAAMRDVVHVAAAISGR
                     MPASLREQHA"
     gene            4240..5589
                     /gene="glmM"
                     /locus_tag="VFR82_08240"
     CDS             4240..5589
                     /gene="glmM"
                     /locus_tag="VFR82_08240"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU4501627.1"
                     /translation="MRKLFGTDGVRGTANLEPMTSETAMQLGRAAAHIFMRRAGRHQI
                     VIGKDTRVSGYMLEAALMSGICSMGVDVLLVGPMPTPAIAFLARSLRADAGVMISASH
                     NPYQDNGIKFFSNDGLKLPDEIEARIEELIVSNEIAHLRPTADAIGKAFRIDDAEGRY
                     IEFVKRSLPKELDFQGIKLVVDCANGAAYKVAPTVLRELGAKVDVIGNKPDGMNINAG
                     CGAVHPTLLQRAVKEYGAHLGIALDGDADRAIFVCEQGNVIDGDHIMAALGLDLHRKG
                     QLAKQTVVGTVMSNFGLELALNRAGLRLLRTAVGDRYLLERMLGDGYNFGGEQSGHFI
                     FLDHNTTGDGLISALQVLSLMKRTEQPLSELAKAMTAVPQVLLNVKVSKKPHLDSIPD
                     LQQAIAESERRFNGSGRVLVRYSGTEPVLRIMIEGEHDSLIREEADRLARIVTAHLS"
     gene            5638..8175
                     /locus_tag="VFR82_08245"
     CDS             5638..8175
                     /locus_tag="VFR82_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU4501628.1"
                     /translation="MLPSLTLAFIGGLALGSLVPYFPLSIALGLVGAVLGSAVLQVRG
                     RSTAGMTTAHYGCLLAGILYWFVSVEASSGPAIVENEPETFETRSGRIISPVQLAPDR
                     MSMVIRCDPTPASAGQPLAVRLTWRAPDRPLFEGDRIRLRARLRAPSGSSNPGGFDYA
                     AYLERQGIDAVATVTGVEAIEFLESGGDSPHWVVWNQFDRWRASIRRSALESLSQPAL
                     GLYLGVIIGERGYLEPEVRDQFMVTGTVHLLSISGSHLGLVAMITFVAVRRGLLLLPA
                     SWLLALSRRITPTKVAAVATVVPVTAYACLAGAEVATVRSLLMVLVALMAKWLGYERR
                     MFHALSAAALIIVAHDPQAIYDISFQLSFLSVSAVAWWLAKTQGAEDHNAPPRSKRAL
                     IGQWISDALLMSAVVTVVTIPLVALYFNQFPWLGLLTNLAAVPLMGGILVPMGLAAVL
                     WQGVFGGGHLPLAEVIQWSMDALVSALRWCSVLPGAEWHGASPSLPSLVLFYGCLAAL
                     TIWSDRKSIRWIAGSGLLMLLVWWLWSPRLMLDGDRFRITFLDVSQGDSAVLELPDGE
                     VVLIDGGASYERFDMGRSVVAPYLWNRGIRTIDYVIATHPQLDHVGGLTYVLGHFNVR
                     HVWGSGDARQELFYQRLTAALAQEGLTEHLVRAGQDTATLGDCALKVLNPFSPAMQSP
                     ASSRRLEGHRLNNRSVVTQLTCGTHKMIFAGDVEHEALSRMSQDGQSERVDVVKVPHH
                     GAGSSLQPKWLDRINPAYAVISVGRHNSYGHPADAVLQAYRARGISVFRTDRDGGVWL
                     TGRRSQPALKIHRMKDERIQPLSFPACSWACELSNWERLLHRWMEAS"
     gene            8348..8548
                     /locus_tag="VFR82_08250"
     CDS             8348..8548
                     /locus_tag="VFR82_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501629.1"
                     /translation="MAKLVRIVIQLPLDLKTRLDGLKQQGYTTSGFIRAMLERELAKI
                     DEQVPEQKSLTLVPTIKRVNSR"
     gene            8627..9655
                     /gene="mtnA"
                     /locus_tag="VFR82_08255"
     CDS             8627..9655
                     /gene="mtnA"
                     /locus_tag="VFR82_08255"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEU4501630.1"
                     /translation="MVPTVEWHKGAVRLLDQSRLPETVAFLDCRDVQAVASAIRELKV
                     RGAPAIGVTAAMGVALGAQAIRVTEYDAFVKALLPICDQLAATRPTAVNLFWAIDRMK
                     RKLSALGSESVSSIKAALLCESQSILDEDIALCKAMGRHGADLIQGGQTILTHCNAGA
                     LATAGYGTALGVIRAAWEQGKKIEVIADETRPVLQGARLTAWELMQDNIPVTLITDNM
                     AGSLMRQGKIHLCVVGADRIAANGDVANKIGTYSVAVLAKAHGIPFYVAAPYSTIDLN
                     TKSGADIPIEQRNALEVTTIHGSQPVAPTGVKVYNPAFDVTPAELISGIITERGVFKP
                     QDLAQRFG"
     gene            9815..>9918
                     /locus_tag="VFR82_08260"
     CDS             9815..>9918
                     /locus_tag="VFR82_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU4501631.1"
                     /translation="MSERTLAIIKPDAVNKHVIGDIVSRYEKAGLKPVA"
BASE COUNT         2051 a         2751 c         3011 g         2105 t
ORIGIN      
        1 gtggataact tgtaccggtc gacggtgatc gtcgaacccg gtgggaatca gcggtttgta
       61 cgacaggcgt ttacccgcct tcctgcggca ctgcagcgac gcctggtgcg gcggagtctt
      121 cgcgcgggcg accccgaagg acgggcgccg aatctgcgta tagtcgaaag cgtgctgcgg
      181 ttcgcggccg gtgagtccaa ggggatgctt tcgatccgag ggtttgacgt gatcacagag
      241 cgtgagtgga tcgtcctccg gctgacttcg caacgaacgg cctccagcac gtccgggcaa
      301 gacctcccgg cagtgactgt gccggtttcc cttcctgcca cggtgtattg gcctggtacg
      361 cgacaacaaa ttcatgtaca agtgatgacc aagcaggcgg ccgcgtcgtg gttgaaccag
      421 cgaaggccgg agtgcgcggt gttcgatgcc gaccggttgt ccgcgccgct gatcatcagg
      481 gcgtggcagg cgggcgatcg gatccatccg cgtggtatgc gtgggaaacg caagaaactg
      541 caggatctct tcaccgactt gaaggtgagc cgggaagagc gcgatcgtat tccgctcatc
      601 gtcgcctcag aaggcatcgt ctgggtggtc ggccatcgac agcatgagcg gtgtgccgtc
      661 gggaagaaca cccgccgctg tcttgtcgtt accgttcaat cgaaagcagg cagtgaagga
      721 gccggataag atggagcgca tcttcggacg gccgatcgtg acgcaggagg aaatgcgtgc
      781 gcgcatcaag gagctcggca aacagattgc caatgactat gccggcaaag atctcgtgct
      841 cgtcggcgtc ctcaaaggcg cctacgcctt ctatgcggat ctcgctcggg ccattcggat
      901 tcccatgcgc gttgattttc tcatcgtcgc gagttatggc agtcaatcga aaacgtcggg
      961 aaaagtaaaa gtgatcaccg agctgacgga gcaaatcaag gggaaggatg tgttgctcgt
     1021 cgaagacatt gtcgattcgg gattgactgt ggagtacttg atcaagacct tgagcaaaca
     1081 aaagcctaaa tccattaaag tctgcacctt gttgagcaag cctgaacggc gaacgatcga
     1141 cgtcccgctt gattatgtgg gattcaagat tcccaacaaa tatgtggtgg gctacgggct
     1201 tgattatcaa cagaaatacc gaaaccttcc ttacctcgcc gagctggaca tggaaggcga
     1261 gcaggagtaa ccgggagccg cacggttgtc agttcgaaga gggctgtggt aacatcactc
     1321 aagctcgcgt atttcctggc gacttgagga agccaggcat aaggagaagt ggatgaattc
     1381 tcgggcaaaa aatctgctct tctgggtcgt tgtcggcctg ttcatgattt tactgttcaa
     1441 cctgttcagt gtaccgactc atgccccgga ggaagaagta attttcagcg atttcatggc
     1501 caagctcgat aagggtgaaa tcgaaaaagt catcatcaag tccagccacg tcagcgccgt
     1561 cttgaaggac aagacccgca tccgcacctt caccgccgaa tacccggatt ttgtgaaggt
     1621 gttgcgcgaa aaaggcgtgc agatcgaagc aaagccccca gacgaaagtc cgtggtacat
     1681 cacgttcctc gtgacgtggg gacccttcgt gctcttcctg ggtctctggt tcttcttgat
     1741 gcggcagatg caaatcggcg gtaacaaggc tttgtccttt ggcaagagcc gcgcgcgtat
     1801 gttgaccgaa gaacggaaaa aagtgacgtt ctcggatgtg gccggcattg atgaagcgaa
     1861 ggaagaagtc ctcgagatca tcgaattctt aaaagatccc cgcaagtttc aaaagctggg
     1921 cgggcgcatt ccgaaaggtg tcttgatcgt cggtccccca ggaacgggaa aaaccctgct
     1981 ggccaaagct attgccggcg aagcaggggt gcctttcttc agcatcagtg gatcggactt
     2041 cgtggaaatg tttgtcgggg tgggtgcatc gcgagtccgc gatctcttcg aacagggcaa
     2101 aaagcatgcg ccctgcatca ttttcatcga cgagatcgat gcagtgggcc gtctccgagg
     2161 cgccggtttg ggtggcggac acgatgaacg cgaacaaacg ctgaaccagt tgctcgtcga
     2221 gatggacggg ttcgacacga cggaaggtgt gatcttgatc gcggccacca accggcccga
     2281 cgtgcttgac cctgcgttgc tgcgcccggg acgcttcgat cggcaagtgg tcgtcaatcg
     2341 gccggatttg cgcggccgct ccgaaatctt gaaggtgcat acgaagaaag tgccgattgc
     2401 gacgaacgtc gaactggaaa agatcgcacg cgggacaccg ggcttctcgg gcgcggacct
     2461 ggaaaacctt gtgaacgaag cggcattgtg ggcggcgcgt caaaacaaaa aggaagttga
     2521 agtcatcgat ttcgagatgg ccaaagataa ggtgctgatg ggcgccgaac ggaagagcat
     2581 gatcctcagc gacgaagaaa agcggacgac tgcgtatcac gaggcgggtc atgccttaat
     2641 ggccaagctc ctgcccggca ccgatcccgt acacaaagtc acgattatcc cccgcggacg
     2701 cgcattgggc gtcacgatgc agttgccgac cgacgaccgg cacaactatt caaaagaatt
     2761 cctgtacaac aacctcgcca ttctcatggg cgggcgcgtg gcggaggagc ttgtgttgaa
     2821 acacatcacc accggtgcgg gaaacgacat cgagcgcgcg acggatcttg ccagaaaaat
     2881 ggtctgtgaa tggggcatga gcgaaaagct tgggccgttg accttcggca ggaaagagga
     2941 ggaaattttc ctcggccggg agttgaccac aaaacgggat ttcagcgatc aagtcgcatt
     3001 ggaaatcgat cttgagatca agcggctggt taccgaaaat tacgaacggg ccaaacggct
     3061 cctcactgag cacatgagaa gcctgaaagc gcttgcggaa gccctgttgg aaaaggaagt
     3121 gttggatgct ccggagatcg atcagatcct gatgcagaac tcctcgcaaa ccgtgcctgc
     3181 ctaaattgct gccagcctcg gtgcagcttc agggcactgg agttgcagag gatcttacct
     3241 cgtgcacttt agaaacctag tctgctgcac tgccccgata tttcaacgag gtaggtgagt
     3301 atgacccgtg tcatgaaaga tacaacactg tatcttgatt ccaacagcat acttaatctc
     3361 gccgcatcgt actcgtaact gtaaccatgg aactcaactt cctagagcga cgcctttccc
     3421 tcgatcgccc cttgctgatg ggcattgtca atgtgacgcc tgattcattc ttcgatggcg
     3481 gacgttttga tgatcctcgg acggcggtgg agcatggacg gcgtttggtc gaggaaggcg
     3541 ctgatctcct cgacatcggt gccgaatcca ccaggcctgg ctcagttcca atcgaccaag
     3601 aggaagagca gcgtcgactc cttccagtcg tcacggcgct tgcacaggcc gtgtccgttc
     3661 ccatctccgt cgatacctcg aaagccgctg tcgccagggc cgcaatcaag gcgggcgccg
     3721 tcatggtgaa tgatgtgact gcgttacgtg gcgacccggc catggtggac gtcgtggcgc
     3781 agacgggagt cgcccttgtt ctgatgcaca tgcaggggat tcctcaaacg atgcagcagg
     3841 cgcccaccta cagggacgtt gttgaggaag tcgctgattt tttagccgag cgagcgcgct
     3901 tcgccatgga gcgcggagtc gcgcaatcac ggattgtgct tgatccgggc atcggatttg
     3961 gtaaagtcct cacgcacaac cttgacctcc tcgcgcatct gcgcatgttg accaccttgg
     4021 ggtttccggt gttggtcggt ccatcaagaa aaggtttcat cgggcagttg actaaacaat
     4081 cggtcgaggc acgggcctgg ggaaccgctg gcgtcgttgc cctggcagta gaacaagggg
     4141 cgaacattct gcgtgtgcac gacgtggcgg cgatgcggga cgtcgtgcac gtggcagcgg
     4201 cgatttcagg tcgaatgccg gcttcattac gggaacagca tgcgtaaact gttcggaaca
     4261 gacggcgtac gtgggacggc gaatctcgag ccgatgacca gtgaaaccgc catgcaactg
     4321 gggcgtgctg cggctcacat tttcatgcga cgagccgggc gccatcaaat cgtgatcggc
     4381 aaggacacgc gcgtctcggg ctacatgctg gaagctgcct tgatgtcggg catctgctcg
     4441 atgggcgtcg acgtgctgct ggtcgggccg atgccgaccc cggctatcgc gtttttggcc
     4501 agaagtctgc gggccgacgc cggcgtcatg attagcgcct cacacaatcc gtatcaagac
     4561 aacggcatca agtttttttc caacgacggg ctcaagctgc cggatgaaat cgaagcgaga
     4621 atcgaggagc tcatcgtctc gaatgaaatc gcccatctcc gtccgacggc cgatgccatc
     4681 gggaaggcct tccgcatcga tgacgcggag gggagataca tcgaattcgt gaaacgctcc
     4741 ctccccaaag agctcgattt ccagggcatc aagctcgtgg tcgattgtgc caatggcgct
     4801 gcctacaagg tcgcaccgac cgttctgcgc gagttgggcg cgaaggtgga cgtgatcggt
     4861 aataagccgg acgggatgaa catcaatgcc ggctgcggcg cggtgcaccc gacgctgctt
     4921 cagcgggccg tgaaggaata cggcgctcac ttaggtattg ccctcgacgg cgatgccgac
     4981 cgcgccatct tcgtgtgcga gcagggcaac gtcatcgacg gcgatcacat tatggctgcg
     5041 ctgggactcg atctccaccg caagggtcag ctggcgaagc agaccgtcgt gggaacggtc
     5101 atgagcaatt ttggcttgga gctggcgctc aaccgggcgg gcctccgatt gctccgcacc
     5161 gccgtcggcg accgatactt gctggagcgg atgttgggag atgggtataa cttcggcggg
     5221 gagcaatccg gtcatttcat ttttctcgat cacaatacga ccggcgacgg gctgatttct
     5281 gcgctgcaag tgctctcgct catgaaacgg actgaacagc ccctgtcgga gctggcgaaa
     5341 gccatgacgg cagtccctca agtcttgcta aacgtcaaag tgtcgaagaa accacatttg
     5401 gacagcatcc ccgatttgca gcaagcaatt gccgaaagcg aacgccgttt caacggtagc
     5461 ggtcgtgtcc tggtccgtta ttccggcacc gaacccgtgc tgcgcatcat gatcgaaggc
     5521 gaacacgata gtctcatccg cgaagaggcc gatcggttgg cacgaatcgt cacggcccac
     5581 ctcagttagg ttcctgggtt cagccacatc acctccatca ccgctggaac ggaagcgatg
     5641 ctgccgtccc ttacgcttgc cttcatcggc ggccttgcgc tgggctctct cgttccctat
     5701 tttccgttgt cgattgctct cggattggta ggggccgtcc tcggatcggc cgtgctccaa
     5761 gtgcgtggac gctctacggc cggtatgacg actgcccact atggctgctt gttggcgggg
     5821 atcctctatt ggttcgtgag cgtcgaagcc tcctcaggtc cggccatcgt cgagaatgag
     5881 ccggagacgt tcgaaacccg ctcagggcgg attatttcac ccgtccaact ggctcccgat
     5941 cggatgagca tggtgatcag gtgtgatccg actcctgcca gcgctggtca gccgctcgcg
     6001 gtccgtctga cgtggcgtgc cccggatcgg ccgctcttcg agggtgatcg catccgactt
     6061 cgagcacgat tacgggcgcc gagcggctcg tccaaccctg ggggattcga ttatgccgcg
     6121 tatctcgagc gtcaaggcat cgatgccgtc gcgacagtta ccggcgtgga agccatcgag
     6181 ttcctggaat cgggagggga cagcccgcac tgggttgtgt ggaatcaatt cgaccgatgg
     6241 cgggcatcga ttcgtcggtc cgctttggag agtctgtctc agcctgccct cggcctttat
     6301 ctcggggtca tcatcggcga gcgaggatat ctggagcccg aggtccggga ccaattcatg
     6361 gtgacgggga ccgtacacct cttatcgata tccgggagcc atcttggctt ggtcgcgatg
     6421 atcaccttcg tggcggtcag gcgcggcctg ctgctgcttc ctgcctcgtg gctcctggcg
     6481 ttgtcacgac ggatcacgcc aacgaaggtt gccgctgtcg cgactgtcgt gccggtgacg
     6541 gcgtatgcct gtttagccgg agcggaggtg gcgactgttc gctcgcttct catggtactc
     6601 gtcgccttga tggccaaatg gctgggctat gaacggcgga tgttccatgc cttgtccgcg
     6661 gcggccttga tcatcgttgc gcatgatccc caagccattt acgacatctc attccagttg
     6721 tcgtttcttt cagtgagtgc tgtcgcctgg tggcttgcga aaacccaggg cgctgaagac
     6781 cacaatgcgc cgcctcggtc gaagcgtgcg ttgatagggc agtggatatc agatgcgctc
     6841 ctcatgagcg cggtcgtaac cgtggtgacg atcccgctgg tagcgctgta cttcaatcaa
     6901 ttcccatggc tcggcctgct cacgaatctc gctgcggtgc cgttgatggg gggcattttg
     6961 gttccgatgg gacttgccgc cgtgctctgg caaggcgtat tcggaggagg gcacctcccg
     7021 ttagcagaag tgatccagtg gtccatggac gcgctggtct cagccctgcg ttggtgctcg
     7081 gtcctgcccg gcgccgagtg gcacggggcc tcgccatccc tcccgtctct cgtgctcttt
     7141 tacggatgtc tggccgcact gaccatctgg tccgatcgaa aaagtatccg gtggatcgcc
     7201 ggaagcggtc tcctgatgct cctcgtctgg tggctgtggt caccaagact catgctcgat
     7261 ggcgatcgct ttcgcatcac gtttttggat gtgtcgcagg gcgatagtgc ggtgcttgaa
     7321 ctaccagacg gtgaggtcgt gctgatcgat ggcggagcca gttatgagcg gttcgatatg
     7381 ggacgcagtg tggtggcacc ctacctatgg aatcggggaa tcaggacgat cgactatgtg
     7441 attgcgacgc acccccaatt ggaccatgtc ggcgggctca cgtatgtgct tgggcatttc
     7501 aacgtgcggc acgtgtgggg aagtggagat gcgcgccagg agctttttta ccagcggctc
     7561 acggcggcgc tggcgcagga aggcttgacc gagcacctgg tccgtgccgg ccaggacacg
     7621 gccacgctgg gcgattgcgc cttgaaggtc ttgaatccgt tctctccggc tatgcagagc
     7681 ccggcgtcgt ctcgccgtct cgaggggcat aggctcaaca atcgctctgt cgtgacgcag
     7741 ttgacctgcg gcacccacaa gatgatcttt gccggcgatg ttgaacatga agccttgtcg
     7801 cgcatgagcc aggacgggca gtctgaacgg gtggatgtgg tgaaagttcc gcaccatggc
     7861 gcaggaagtt cgttgcagcc caagtggttg gatcggatca atcccgccta tgccgtcatt
     7921 tcagttgggc ggcacaattc ctatggccac ccggctgacg ccgtcctgca ggcctatcgc
     7981 gctcgcggga tttcagtctt tcgcacggac cgggacggcg gcgtctggtt gacgggcagg
     8041 cgctcgcagc cggcgctaaa aatccatcgt atgaaggacg aacgcattca accactgtct
     8101 tttcctgcct gctcatgggc ctgtgaacta tcaaactggg aacgcctgct ccaccggtgg
     8161 atggaggcat cgtgaccgga tcttgtacat ttgagggcac gtgcagacga aattaggcaa
     8221 acaagtcgag ggcaggcatc tttttatcgc taagtcctca attctacaca gcgccatatt
     8281 cgttccagca aggcatagcg tgtgcaaacg cccctacctg gatcagcttc tgtataaggg
     8341 gtctcacatg gcaaagcttg tccgtatcgt gatccagctt ccgctagatc taaaaacccg
     8401 gttggacggc ctcaaacagc agggttatac gaccagcggg ttcattcgtg ccatgctgga
     8461 gcgtgaactc gcaaaaatcg acgagcaagt ccctgagcag aagtccttga cgctcgttcc
     8521 aacgatcaag cgggtcaaca gtcgataatc ctctctcatt tccccggttc atcctgaacc
     8581 cagcctcttg cgctgcccat gcgaatcccg ttacatagtg tcgcctatgg ttccgaccgt
     8641 tgaatggcat aagggggctg tacgcctgct cgaccagagt cgccttccgg agacggttgc
     8701 cttcctggac tgtcgagatg tccaagccgt ggcatcggcc attcgggaat tgaaagtgcg
     8761 cggcgcgccg gcgattggtg tgactgccgc catgggagtg gcgctgggag cccaagccat
     8821 ccgcgtcacc gagtacgatg ccttcgtcaa ggcgctgcta ccgatttgcg atcagctggc
     8881 cgctacacgg ccgacagccg tcaatctttt ctgggctatc gaccggatga aacgcaaact
     8941 ctctgccctg ggttccgagt cggtctcgtc gatcaaagcg gccttattgt gcgagtcgca
     9001 atccatactc gacgaagata tcgccttgtg taaagcaatg ggtcgccatg gcgccgacct
     9061 cattcaggga ggccaaacca tcctcactca ctgcaatgcc ggcgccttgg ccacggcggg
     9121 gtacgggacg gcgttgggtg tcatccgcgc ggcgtgggaa cagggaaaaa aaattgaggt
     9181 cattgccgac gaaacgcgac cggtcttaca aggagcgcgc ctcacggcat gggagctcat
     9241 gcaggataac attccggtta cgctcatcac ggacaatatg gcagggtcct tgatgcgcca
     9301 gggcaagatt catctgtgtg tggtcggggc ggaccgaatt gccgcgaacg gggacgtggc
     9361 gaacaagatc ggtacttact cggtggcggt cttggccaag gcgcacggca taccgttcta
     9421 tgttgcagca ccgtattcaa caatcgattt gaacacgaaa tccggcgccg acatcccgat
     9481 cgaacagcgc aatgccttag aagtcaccac cattcacggt agtcagcctg tcgcgccgac
     9541 cggcgtcaaa gtgtacaatc ccgcgttcga tgtgacgccc gctgagctca tttccggcat
     9601 cattaccgag cgcggcgtgt tcaaaccgca agacctcgcg cagcgctttg gatgatacct
     9661 atggcagcag gagccgcgct cgcgaaggtg cgggtttgcg gtgcaggagc gatgcggtcg
     9721 ctgtccctcc cttgaaactc cgcattccaa gcccctataa tgccgcgaca ttttttgcac
     9781 tttttgtctc gagattggag gaagacttca agcaatgagt gaacgcacgc tcgcgatcat
     9841 caagccagac gccgtgaaca aacatgtgat cggtgacatc gttagccggt acgaaaaggc
     9901 cggtttgaag cctgtggc
//
LOCUS       DASTQY010000249         3748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_207164, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000249 DASTQY010000000
VERSION     DASTQY010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207164"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..114)
                     /locus_tag="VFR82_08265"
     tRNA            complement(38..114)
                     /locus_tag="VFR82_08265"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78..80),aa:Arg,seq:tcg)
     gene            complement(174..677)
                     /locus_tag="VFR82_08270"
     CDS             complement(174..677)
                     /locus_tag="VFR82_08270"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU4501632.1"
                     /translation="MNIVLIGYRGTGKSAIGKILAAQLGRQLVSTDEEIIRRAGKSIP
                     DIVAAHGWDYFRDLETAVCQDLAGRDNLVIDTGGGAILRHENVKVLKGNGRLFWLTAS
                     VETIAARIGGDSQRPSLTGTKSHVAEIRDVLSERTPKYQAAADQSIPTDGRSVEELAR
                     TILKQVS"
     gene            complement(751..1605)
                     /locus_tag="VFR82_08275"
     CDS             complement(751..1605)
                     /locus_tag="VFR82_08275"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU4501633.1"
                     /translation="MDINTATQFCGLIGNPIEHSLSPAIHNAAFQKLALNFVYLAWKV
                     DKIGDAVCGLRALGNFRGASVTIPHKVAVLPFLDEIDATARHIGAVNTIVAEQGKLLG
                     MNTDATGALRALREGNAPLKGASVVIVGSGGAARAIAFALAAEAEVRCLHLLGIEVDE
                     RRNLATDLRDKTGVSVGEADLDEGRLKDVLPEARVLVHCTPVGMAPKIGRSCVPPKFL
                     HRELTVMDVVYNPRETQLLTDAKTAGCRTISGLEMFLYQAVAQFERWTGRSAPIQVMR
                     AVLESRFP"
     gene            complement(1645..2406)
                     /locus_tag="VFR82_08280"
     CDS             complement(1645..2406)
                     /locus_tag="VFR82_08280"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4501634.1"
                     /translation="MNRLDGKVAIVTGGNAGIGEAVAKRFADEGAAVVVTGRRQVELD
                     RVISGIRHNKGKALAVTGSVTDEAHVRDVVQRALDSFGRIDILVNNAGIGDFGKRLHE
                     TDDSAWATVVDVNLTGVFRITRAVVPHMLKQGRGAIVNISSVASLVGIPGAPAYAASK
                     GGIDALTRAMAIDYAQDGIRCNVVNPGLVDTPMAAPLMANPEMLRPILAHYAIRRPGT
                     SEEVANMVLYLSSDEAGWVTGGTFTIDGGMTVSKG"
     gene            complement(2387..3280)
                     /locus_tag="VFR82_08285"
     CDS             complement(2387..3280)
                     /locus_tag="VFR82_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEU4501635.1"
                     /translation="MSSATLQNGDRIHLVDRKGRQYALTLKAGDIYQISGHKLAHDDL
                     INKPDGSVVHLSGGKSMLAMRPTFGEYVLKMPRGAQVLYPKDLALITMWADVYPGARV
                     FEAGTGSGALTMALLRAIGPRGVVVTYEAREDFARTARSNIERYVGPVPNLIALRKNA
                     YEGIDLLEDGVPFDRLVLDLPEPWQVIPHAARALRSGGIYLSFVPTVPQVQQTVTALQ
                     RAAVFGMVETFETLLRTWSVEGRSVRPDHRMVAHSGFLTVARKVEPGFCTPAQGLQMT
                     TDEDMQGETDGKDQGHESSGR"
BASE COUNT          825 a         1129 c          963 g          831 t
ORIGIN      
        1 gcgagaagga gggcccaatc taaccatttg atttctttgg tgcgcccggc aggaattgaa
       61 cctgcgacct gcgggttcga agcccgacgc tctatccaac tgagctacgg gcgcactcag
      121 ctacttcaag cactttgctt gagacattgt caaggttatg ccgagggcac caactaggac
      181 acttgcttca gaatcgttct ggcaagttct tcgaccgaac gcccgtccgt cgggattgac
      241 tggtccgctg cagcttggta cttgggtgtt cgctcgctca atacgtcgcg gatctcagcc
      301 acatgggatt ttgtccccgt cagcgacggc cgttggctat cgccaccgat ccgagcagca
      361 atggtttcca cggaggccgt caaccagaac agtcggccgt tgcctttcaa tactttgaca
      421 ttttcgtgac gcaagatcgc ccctccccca gtgtcgatca ccaaattgtc ccggccggca
      481 agatcctgac ataccgctgt ttcgagatcg cggaagtaat cccagccgtg tgctgcaaca
      541 atgtcgggaa tcgatttccc ggcgcggcgg ataatctcct catcggtcga cacgagctgt
      601 cgccccagtt gcgcagcgag gatcttcccg atcgcgcttt ttcccgtccc tcgatacccg
      661 atcaagacga tgttcattgc attcactcgt gatatccgtg tgaggacgca tgagaatgct
      721 cgtaggtgag ggcgcctcac agcacgaaag tcatgggaat cgagattcta agacagcgcg
      781 catgacttga ataggagccg atcgacccgt ccagcgctcg aactgtgcca ccgcttgata
      841 gagaaacatt tcgagccccg agatcgtgcg acacccggct gtttttgcgt cggtgagcag
      901 ctgcgtttcg cgcgggttgt acacgacgtc catgaccgtc aattctcgat ggagaaactt
      961 gggtgggaca caactcctgc cgatctttgg cgccatgccg accggtgtac agtggacaag
     1021 cacacgagcc tctggcaaaa cgtccttgag gcgaccctca tcgaggtccg cctctccgac
     1081 gctcactcct gtcttgtccc tcaagtccgt tgcaagattc ctgcgctcgt ccacttcaat
     1141 tcccaacaaa tgcaagcaac gcacctcagc ttccgccgca agggcaaaag caatcgcgcg
     1201 cgcggctccg cccgagccga caatgacgac ggatgctccc ttcaatggag cgttgccttc
     1261 ccttaatgca cggagcgccc ccgtcgcatc cgtgttcatg ccgagcaact ttccttgttc
     1321 agcaacaatc gtgttgaccg cgccaatgtg ccgcgccgtc gcgtcaattt catcgagaaa
     1381 gggtaagacg gcgactttgt gagggatggt gacgctggcg ccgcgaaaat tccccaaggc
     1441 acgtagaccg caaacggcat caccaatttt atctaccttc cacgccagat aaacgaaatt
     1501 gagtgccaac ttctgaaatg cggcattgtg aatggcaggg gagagagagt gctcgatggg
     1561 attcccaatg agtccgcaaa actgtgtggc ggtgttgatg tccatgacgc tcgtgagact
     1621 gaacgacgta tcagcgtaga atactcatcc cttggaaact gtcatgcccc cgtcaatcgt
     1681 gaacgtccct cccgtaaccc aaccggcttc atcagaagac aggtacaaca ccatgttcgc
     1741 gacctcctcc gaagtccccg gtcttcgaat ggcgtagtga gccaaaatcg ggcgtagcat
     1801 ttcaggattc gccatcaatg gagcggccat cggtgtatcg acgagaccgg gattgaccac
     1861 gttgcagcgg atgccatcct gggcataatc aatcgccatg gcgcgggtga gcgcatcaat
     1921 cccgcctttt gatgccgcat aagccggcgc tcctggaatc ccaaccaggc ttgcaaccga
     1981 ggaaatattg acaattgctc cccgtccctg tttgagcatg tgaggaacga cggcccgtgt
     2041 gatgcgaaag acccccgtca aattcacatc aacgaccgtt gcccaggcag aatcgtctgt
     2101 ctcgtgaagt cgcttgccaa aatcgccgat gccagcgttg ttcaccagga tatcaatgcg
     2161 cccaaaactg tcgagcgccc gctgcaccac atcccgcaca tgcgcttcgt cagtcactga
     2221 gccagtgacc gccaatgcct ttcccttgtt gtgcctgatt ccactgatga cgcgatccaa
     2281 ttccacttgt cttctgccag tgacgacgac ggcggcccct tcatcggcga agcgctttgc
     2341 aaccgcttct cctatgccgg cgttgccgcc ggtcacaatc gccaccttac cgtccagacg
     2401 attcatgtcc ctggtccttt ccatccgttt ccccttgcat gtcctcgtca gtcgtcatct
     2461 ggagaccttg agcgggcgtg cagaatcctg gttccacttt cctggccacg gtgagaaatc
     2521 ccgagtgcgc aaccatgcga tgatcagggc gtacactgcg accttcaacc gaccacgttc
     2581 gcaacagcgt ttcaaaagtt tcaaccatac cgaagacggc cgcccgttgg agtgccgtca
     2641 cggtctgctg tacctgtggc acggttggga caaagctcag atagattcct ccggatcgca
     2701 aggcacgcgc ggcatgggga atcacttgcc acggctcagg aagatccagc acgagccgat
     2761 caaatggcac gccatcctcg agaaggtcga tcccttcata ggcattcttg cggagtgcga
     2821 tgaggttcgg caccggcccc acataccgct caatgttgct cctggcggtc ctcgcaaaat
     2881 cctcccgtgc ctcatacgtg acgaccacgc ctcgagggcc tatcgctcgc aagagcgcca
     2941 tcgttaacgc gccagacccg gtgcctgctt cgaagacccg agcgcctgga tagacgtcgg
     3001 cccacatcgt aatgagagcg aggtccttgg gatacaggac ctgcgccccc cgtggcatct
     3061 tcaggacgta ctctccaaaa gtgggacgca tagccaacat gctcttaccg ccggaaagat
     3121 gtacgactga gccatctggt ttattgatca gatcatcatg tgcgagtttg tgcccgctta
     3181 tctgatagat atcgccggcc ttcagagtca acgcatactg gcgtcctttc ctgtcgacga
     3241 ggtgtatccg atcgccattt tgcagagttg cggaggacat gacgcgccat tctagaagcg
     3301 cgacactggc ttttcaacaa ccgcagcttc tccgatacca gggttatccc tgatccttcg
     3361 gaatgagaca aatcgaccca aacgctatct caaagcctgc acattcggag caggattgcc
     3421 tcagcctcct ccaagattcc tcattaagcc gctctaatag tgatgtattt tcactgtgat
     3481 cgcgccgtgc agttctcatg atccatccag catgggactt gctgcgtaga atattcgcag
     3541 taacaatcgt gtgaatcttt tgtgctggga ttgcctctca cctaaagaga cttatttgta
     3601 tgtgatcatt gaaaccgacg acatttcata cgagtagtta ggaggaggac gcttacatga
     3661 aaaacgacct gttctctgcc gatggtccag ggctgaaatc gccggagcta aaagatgcag
     3721 atacggatca ctccgaggac agcgattt
//
LOCUS       DASTQY010000250         8268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_606640, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000250 DASTQY010000000
VERSION     DASTQY010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8268
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606640"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="VFR82_08290"
     CDS             <1..1103
                     /locus_tag="VFR82_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEU4501636.1"
                     /translation="GAGTAEALLALVADDMAAQSKRLTRHGLTLGSSREGLTLTMSPV
                     EENVLIAGTSGSGKSTLAHGILEQLIDKGYQTCVIDPEGDYQSIEKMVMFGNSQRGPT
                     VAEVLTAMDNPSTQVIVNLVGLPLKDRPPFFFELLPQLQERRNRTGRPHRVLVDETHH
                     LLPTKWRATVASMRDLKGMLFVTVHPDQLSPHVLDTVDIAVALGERPGSTLQTVANHK
                     DLSLPHVPNAELKPGEALLWRVAAGQSPETFMMTVSRMERRRHRRKYAEGELPPERSF
                     YFRGPEGKLNLRAQNLIMFRQLADGVDDATWLHHLRNRDYSHWMETAIKDPSLAQVVH
                     DVEDMPDLSAHESRQRVASAIEERYTLPATGV"
     gene            1125..1406
                     /locus_tag="VFR82_08295"
     CDS             1125..1406
                     /locus_tag="VFR82_08295"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEU4501637.1"
                     /translation="MRVLSGMIVGSLALLVSACAETEQGKSYWLRHAVVTLAQAAEIA
                     ETNGPGRAVGVELGQSGNRVFYDVEIIDTMNKSRRLRVDAETGKIVKGM"
     gene            complement(1703..2677)
                     /locus_tag="VFR82_08300"
     CDS             complement(1703..2677)
                     /locus_tag="VFR82_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501638.1"
                     /translation="MEIDRTFSHWSIGTPRIKWSAIFAGLAVGLAVQMILTLAGLGLG
                     AWAVDLHEPSPAEGVPLGTAVWTGLTMLVSAFFGAYMTARLSGTAERTDGVYHAVVLW
                     GVNWLIFAWITTTAMATLIGGAFSVFGTTLQTVQTVGQGLSITASTAVSRTAGSLHLS
                     VDDLRREIESVLQATGKPDLQGGEIPRDADRRAERARQRQPMKEISDQAVIELRDKLT
                     ALDREAAMNILVNKFGMYPAPARDVVQSSIGMLGPIQDTIRGDKQRSTAVGTEAVERL
                     GMLALWLSGLALVSLAVSALGGLIGTPEESLVESTTSTETYRDIRRAS"
     gene            complement(2726..3157)
                     /locus_tag="VFR82_08305"
     CDS             complement(2726..3157)
                     /locus_tag="VFR82_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4501639.1"
                     /translation="MDGYGKRVLVVEDDDDVRTLMSVILRETGYNVYEACDGLEAIDA
                     LKKRRYDVLLTDYHMPKMDGLELLDLAQAMWPELPVILATSDVLLTERTPNSLFESAY
                     AIVEKPFDRAVLLTLIRSAIDRLPKPTLHAMRSHPAYQAFA"
     gene            complement(3321..4676)
                     /locus_tag="VFR82_08310"
     CDS             complement(3321..4676)
                     /locus_tag="VFR82_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU4501640.1"
                     /translation="MDDPANILVVDDDEETVTLLREILSKEGYNVDTAADGKIALDKI
                     NSRPPDLVISDIHMPDLDGMSLLAELRVRYQDVPVILMTAYGSLKTAVDGLRSGAFDY
                     LSKPFIVDDIRLVVRRALEHKNLSRQNQALKEQLKHRYRFDNLVGSSPGIVSVYKSIA
                     RVANTESTVLLQGESGTGKELIARAIHANSARSAGPFVAVDGGSLAETLLESELFGHE
                     RGSFTGAVTSKKGLLEKAHLGTCFLDEVADLSPVLQGKLLRVIQEREIRRVGSTTPMT
                     IDVRMVAASKKDLEQLVKAGSFREDLYYRLNVVTINIPPLRERAEDIPLLTQYFIQMY
                     GGKKSPPVTGISPEAMALLTRYWWPGNIRELEHAIERALALTPHPVIFPEDLPQSIQS
                     ATVQAAAQARGWVTLAELEKDHILRVLEAHNQDLGRAATILGIHRKTLLRKLRYFGFP
                     C"
     gene            complement(4676..6274)
                     /locus_tag="VFR82_08315"
     CDS             complement(4676..6274)
                     /locus_tag="VFR82_08315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4501641.1"
                     /translation="MIDEYPGQALDKRGRLLITIVLLLTVTMATFAADRLLPLGVAVW
                     MLYLVPVVLTTQLLPQYTGLALAISSGLLLVGVLDSKEGISYELAVINRGLGLIVLLF
                     TGLFLLQHRRMAEALRESEGRYRLLLEQHGRARAEAALHEVQERFTSIFRSSHDAIAY
                     ATFDGRLLEVNDAFVRLSGRSREALLSPEPPPSLWASLGLPPAQFEELRETGKPLVYE
                     TISRQDGLSAEWLNVHAFAVRGSDGAISGLAVVVRNISDRKQAEAELRSTSEELRAKN
                     EALARTNELLSAAQHAAARAQRLSAIGQFAATVAHKIGTPLTALSGHVQLLMEDLSLP
                     SKVRGRLQTVEAQIERTSRIIQDLLLYTRRAPPVRTKIDINECLRECASLFRTECERQ
                     HVACITELAPELPPVEADRQHMLEAFNHLIENALQAMPNGGSLCLRTASIEPVTVGRG
                     IHHAGGVAVEIADTGHGIKPDHSAQIFQPFFTTKQAGRGTGLGLAIVQETVRAHDGRI
                     TVESEPGKGTTFTIHLPAVDKGTP"
     gene            complement(6859..8121)
                     /locus_tag="VFR82_08320"
     CDS             complement(6859..8121)
                     /locus_tag="VFR82_08320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4501642.1"
                     /translation="MGSTQLAPQWLPHRVVGRRVTLLASEMGPMNSAQPQAKTDDLDL
                     EEQPRVHVGCGLCGSRRSRLVCSREDVLSQQRFLERFYRCRWSQQNAATATDRVNFTQ
                     DYATAIVACMDCGLLFRNPRPRADTVTQAYETEAYDETYLQAEFTAQQTWARRKADIL
                     ARHLEKVGRRGRPRVLEIGSFVGGFLFEGQLRGWDMLGVDPGHDVASFCRRHRLPVFQ
                     GTLEAAQLPPASFDAVVIWNTFDQLPDPRKLLQRLLPLMRNGGLLVVRVPNGACFEWG
                     IAMRPRLPKVFRRLLEGAMACNNLLTFPYLYGYSAEQLERLTESFGFRLLACHADQLV
                     SPPPGHLRRWALWEERAVKTFCRMLAVLWPDNRSLRFRSAPWLDCIFERACTDEERPA
                     PEKLGLGVIPVYSPLVFEDTGLDCSVNR"
BASE COUNT         1790 a         2372 c         2389 g         1717 t
ORIGIN      
        1 acggggccgg aactgctgaa gcgttgctcg ccctggtcgc cgacgatatg gccgctcaat
       61 ccaagcgact gacccgccat ggcctcacac tcggatcatc acgcgagggc ctcaccttga
      121 ccatgtcgcc ggtggaagaa aatgtgttga tagccggaac atcgggaagc ggaaaatcca
      181 cgctcgctca tggaattctc gagcaactca tcgacaaggg gtaccagact tgtgtcattg
      241 atccagaggg cgactaccag tcgatcgaaa agatggtcat gttcgggaac tcccagcgcg
      301 gccccactgt tgccgaggtg ctgacagcca tggacaatcc atcgacgcaa gtcatagtca
      361 acctggttgg cttgccgcta aaagaccgtc cgcccttctt ttttgaactg ctgccccaac
      421 ttcaggaacg gagaaatcga acgggaagac cccatcgcgt cctggttgac gaaacccatc
      481 acttgttgcc gacgaaatgg cgtgcgactg tggcgtcaat gcgtgatctg aagggcatgc
      541 tgttcgtcac cgtgcatcct gatcaattgt ctcctcatgt gcttgacacg gtggacattg
      601 cggtcgcact cggagaacgg ccgggcagca cgctacagac ggtggcgaac cataaggact
      661 tgagcttgcc tcacgttccc aacgctgaac tcaagcctgg tgaagcacta ttgtggcgag
      721 tggcggcagg acaatctccg gaaacgttta tgatgacagt ttctcgcatg gagcgccgac
      781 ggcatcggcg gaagtacgcc gagggcgaac ttccgcctga acgcagtttt tactttcgcg
      841 gcccggaggg aaaactgaat ttgcgcgctc agaacctgat catgtttagg cagctcgcgg
      901 acggcgttga tgatgcaacc tggctgcatc acttgcgtaa cagagactat tcccattgga
      961 tggagacggc catcaaggat ccgtcgttgg ctcaggttgt ccacgatgta gaggacatgc
     1021 cggatctatc cgcacacgag agccggcagc gcgtggcaag cgcaattgaa gagcggtata
     1081 cattgccggc cacgggagtg tgatgctgtg cagaaaggcg gaacatgcgt gtgctctcag
     1141 gcatgatcgt tggctcgctg gcccttctgg tgagtgcttg tgcggagact gagcaaggga
     1201 agagttactg gctgcgtcat gcggtcgtga cgctcgctca ggcagcggaa atcgctgaaa
     1261 cgaacggtcc agggcgcgca gtcggggttg agcttggtca gtcgggcaat cgcgtgtttt
     1321 acgatgtgga aatcatcgac acgatgaaca agtcacgacg tcttcgggtc gacgctgaaa
     1381 ccggcaagat cgtcaaagga atgtaaccct ctcatgctca ccgaatcggc gccggtggga
     1441 tgggttccgg ttgcggagga ggattggggg ttggtggaat tggaacaccc ggcgggcgtg
     1501 gaataggggc gggtggaatc ggttgcggat caacctgcaa cgccggcggc ctccacgaac
     1561 tcgctggctc actccacgac ggcacaatca gcatggcgcc cagggacaga aggacaccag
     1621 gaatctgcct caactggggc catgccttca tggttcgatc cattttgccc ccgatgatcc
     1681 cgcctgcgtt gctaactgtc ggtcagcttg cgcgccgaat gtcgcgatag gtctctgtac
     1741 tcgtggtcga ctcgactagc gattcttccg gtgtgccaat caggccgccg agtgctgata
     1801 ccgccagact aacgagagcc aggccggaca accagagcgc gagcattccg agacgctcca
     1861 cggcttctgt tccgactgcc gtggaacgct gtttgtcgcc tcggatcgta tcttggattg
     1921 gcccgagcat gccgatagac gattgcacga cgtctcgcgc cggcgccgga tacatgccaa
     1981 acttattcac gagaatgttc atggcggctt cccgatcgag tgccgtcagc ttgtcacgta
     2041 gttcgatcac cgcctgatcg ctgatctctt tcatgggctg cctctgcctg gcacgttccg
     2101 ctctgcgatc ggcgtcccgt gggatctccc ctccctgcaa gtccggcttg cctgtcgcct
     2161 gaagtaccga ctctatctct cggcggagat cgtccacgga aaggtgaaga ctgcccgctg
     2221 tccgcgacac ggcagttgaa gctgtgatcg agagaccctg gcccacggtc tgcacggtct
     2281 gcagagtcgt tccaaagact gaaaacgctc caccgatcag ggtggccatg gccgtcgttg
     2341 tgatccacgc gaatatcaac cagttgacgc cccaaagcac caccgcgtga tagaccccgt
     2401 ccgtgcgttc ggcagttcca gacaagcggg cagtcatgta cgcgccaaaa aatgcggaaa
     2461 caagcatcgt gagaccagtc cagactgctg tcccaagggg cacgccttcg gcgggacttg
     2521 gctcgtgaag gtcaacagcc caggcaccta acccgagacc agccagggtc aagatcattt
     2581 gcacagccag accgaccgcc aatccggcaa atatggcgct ccatttaatt cggggagtcc
     2641 ctatggacca gtgggaaaag gttcgatcaa tttccatggt acgttcacct gccgcaaatc
     2701 caagaccgcg gatcaggtgt ccccttcatg cgaacgcctg gtacgctggg tgcgaacgca
     2761 tcgcgtgaag cgtcggtttg ggcagcctat ctatagccga acgtatcaac gtcaacaaca
     2821 cggcccggtc aaagggcttt tcgacgatgg cataggccga ttcgaataaa ctgttcggcg
     2881 tccgttcggt caataagaca tcactggtcg cgaggatcac cggcaattcg ggccacatcg
     2941 cttgggcaag gtccaagagc tccaagccgt ccatcttggg catatgataa tcggtgagca
     3001 ggacgtcgta gcgtcgcttc ttcagggcat cgatggcttc caatccgtcg catgcttcgt
     3061 agacgttgta gcctgtttcc ctcagaatca cactcatgag cgtccgaaca tcgtcgtcat
     3121 cctcaacgac gagcacccgc ttgccgtacc catccattgc agagcctcct tacaggcatg
     3181 ttgatgattc gattgagcga agcaaggtcc ggaccgagat ctgggcatgt cgaaagcgaa
     3241 atcgcaaata ttcttgtgac ttcaggaaga gaagtgaggt cgtgccggac gccctgtccg
     3301 ggcgtggaca acgtgtccgt ctaacaaggg aagccgaaat aacgaagctt tctgaggaga
     3361 gttttgcgat ggatgccgag aatcgttgca gcgcggccta aatcctggtt gtgggcctcc
     3421 agtactcgaa ggatgtggtc cttttccaat tcggccagcg tcacccagcc gcgggcttgg
     3481 gcagcggctt gtaccgtcgc gctttgaatc gattgcggca aatcttcagg gaagatgacc
     3541 ggatgaggcg tcagggccaa ggcgcgttcg atggcatgtt cgagctcgcg gatgttgccc
     3601 ggccaccaat accgggtcag gagggccatg gcctccgggg aaatgcctgt gacgggagga
     3661 gattttttcc caccatacat ctggatgaag tactgcgtca gcagggggat atcttccgcc
     3721 cgttcacgca ggggaggaat gttgatcgtg acgacattca agcggtaata caggtcttcg
     3781 cgaaagcttc ccgcctttac cagttgctct aaatccttct ttgaagcggc gaccatgcgc
     3841 acgtcgattg tcatgggcgt ggtgctcccg acgcgccgga tctcccgttc ctggattacg
     3901 cgtagcagct tgccctgaag aacgggcgag agatccgcaa cttcgtccag gaaacacgtc
     3961 cccaaatgcg ccttctcaag caagcctttt tttgaggtga cggctccggt aaacgaccct
     4021 cgctcgtgac cgaatagctc ggattccagc agcgtttccg caagtgagcc gccgtcgacc
     4081 gcaacgaaag gaccagcact tcgcgcactg ttggcatgga tggcgcgggc gattaattcc
     4141 tttcctgttc cgctttcgcc ttggagcagg accgtgcttt cagtattggc gacgcgtgcg
     4201 attgatttat agaccgacac gattcccgga ctgctgccta ccagattgtc gaagcggtag
     4261 cgatgtttga gctgttcctt cagcgcctgg ttttggcgag aaaggttctt gtgctcgagg
     4321 gcccgacgaa ccaccaggcg aatgtcgtcg acgataaatg gtttgctcag atagtcgaac
     4381 gctccggaac gaaggccgtc gacagcggtt ttcaaagagc cgtaagccgt catcaaaatg
     4441 acgggcacgt cttgataccg cacgcgcagc tctgccagaa ggctcatccc atcaagatcg
     4501 ggcatatgaa tgtccgaaat aacgagatca ggcggccggc tgttaatctt atccagggca
     4561 atttttccgt cggcggcggt gtcgacattg tagccttcct tggagaggat ctcccgaagc
     4621 aaggtcaccg tttcctcatc gtcatcgacg actagaatgt tggcagggtc gtccatcaag
     4681 gtgttccttt atcaacggcc ggtaaatgaa tagtgaacgt cgtccctttg cccggttcgc
     4741 tctcgacggt gatacggcca tcatgcgctc gcaccgtttc ctgcacaata gccagaccaa
     4801 gacccgttcc gcgaccagcc tgcttcgttg tgaagaacgg ttggaagatt tgcgctgagt
     4861 ggtccggctt gatgccgtga ccggtatcgg cgatttcgac ggccactccg cccgcgtgat
     4921 gtatgcctcg gccgacggtg accggttcga tactcgccgt ccgaagacac agcgagccgc
     4981 cgttaggcat agcttgaagc gcattttcaa tgaggtggtt aaaggcttcg agcatgtgtt
     5041 ggcgatccgc ctcaaccggc ggaagttcgg gagccaattc cgtaatgcac gccacgtgct
     5101 ggcgttcgca ttcagtccga aagagactgg cacactcccg caggcattcg ttgatgtcga
     5161 ttttcgtccg taccggtgga gcacgtcgcg tatagagcag cagatcctgg atgatccgag
     5221 acgtgcgctc gatttgcgct tcaaccgtct gcagtcgccc ccggactttt gaggggagtg
     5281 ataaatcttc catgagcagc tgcacgtgcc ccgacaacgc ggtcagcggc gtgcctatct
     5341 tatgagccac cgtcgcggca aattgtccga tcgccgaaag gcgctgcgcc ctcgccgccg
     5401 cgtgttgggc ggcagacagt aattcattgg tgcgggcgag agcttcgttc ttcgcgcgca
     5461 gctcttcgct cgtacttcgc agttccgcct cagcctgttt ccggtcgctg atattgcgaa
     5521 ccacgaccgc taagccggaa atggcgccat cggaccccct gacggcaaat gcatggacat
     5581 tcaaccattc agctgacagt ccatcctgac gggaaattgt ctcgtagacc aacggtttac
     5641 ctgtttcccg gagctcttcg aattgcgcag gcggcaaccc cagagatgcc cagagcgatg
     5701 ggggcggctc gggactcagc aaggcctccc gcgaacgacc ggataagcga acgaacgcgt
     5761 cgttgacctc gaggagcctg ccgtcgaacg tcgcataggc gatggcatcg tgtgacgaac
     5821 ggaagatgct cgtgaagcgc tcttgcacct cgtgcagcgc cgcttctgct cgagcgcgtc
     5881 cgtgctgctc gagaagcagg cggtagcgac cttcgctctc ccgtaaagcc tctgccatcc
     5941 gacggtgctg aaggagaaac aaccccgtga acagcagcac gatgaggccc aagcctcggt
     6001 tgatcaccgc caattcgtat gaaatcccct ccttcgaatc aagcacgcct acaagcagca
     6061 gtccgctgct aatggccaac gccaggccgg tgtactgcgg cagtagctgg gtcgtcagca
     6121 cgacgggaac cagatacagc atccacaccg ctaccccgag tggcaagagc cggtcggcgg
     6181 cgaacgtcgc cattgtcacg gtgagaagga gcacaatcgt gatcaagagt ctgccgcgtt
     6241 tatcgagggc ttgtcctggg tactcgtcaa tcatgttcct gtctcgttcg ggacaagttg
     6301 tcccgtggct cgctctggtt ccgcagtctc gtacactgac tcaatcggaa ttcttcgcca
     6361 tattttcaac catgtaggag gaaatttcat gcgtggcagc ctttgtgctt ctaacactga
     6421 aacaggaagg gcggaaaccg acgccgcctg acaaccaacc tctatccaag gagatgcgag
     6481 catgagttgc gagcgatgcg gaggtttcaa ggtattcgac tacttcgacg gcccttttca
     6541 ctgcagcggc tatcgttgca tcaattgcgg ggcaatcacc gatatgcgga tcgccatgtc
     6601 cacgcaaact cggacagtcg tgcctcccag gcgggtgaag accgctcgtc gtccttcatt
     6661 tgtcgcgccc gagtctgggg ctgctcatcc gggtcagatt gaaaaccagc catgaatcta
     6721 caccgcgacc cgaccgtgcc ggttgctgtc ttgcgccggc acgacggagg gtccccgagc
     6781 tgaacagttt tgaaaccagt aaagacgtga aagaagtctt cggaatcatc gctttcatgt
     6841 gaagcctcct ttccgatcct accgattaac cgaacaatct aggccagtgt cctcgaagac
     6901 taggggggag tatactggga taacccctag gcctaacttc tcaggtgcag gacgctcttc
     6961 gtcagtgcaa gccctttcaa aaatgcagtc caaccacggc gcggaacgga atcgaagcga
     7021 tcgattgtcc ggccagagaa ctgcgagcat tctgcaaaag gttttcaccg cccgctcctc
     7081 ccataaggcc caccgacgca gatgacccgg gggaggtgaa acaagctgat cggcatgaca
     7141 ggccagcagc cggaagccga acgactctgt gaggcgttcg agctgctccg cagagtaccc
     7201 atacaggtag ggaaatgtca ggagattgtt acaggccatt gcgccttcta gcagtcggcg
     7261 gaatactttc ggcaaacgag gccgcatagc tataccccac tcgaaacatg ccccgttggg
     7321 gacccgcacg acgagcaggc cgccgttgcg catcaacggt aacaaccgtt gaagcagttt
     7381 ccgagggtcg gggagctggt caaatgtgtt ccagattaca actgcgtcga atgaggccgg
     7441 aggcagttgg gccgcctcga gcgttccttg gaacaccggt agccgatgcc gacggcaaaa
     7501 tgaagcgacg tcatgacccg gatccacacc gagcatgtcc caccctcgca gctgtccttc
     7561 aaacaggaag ccaccgacaa agctgccgat ttccaacacg cggggcctgc ccctgcggcc
     7621 aaccttttca agatgccgcg caaggatgtc tgctttgcga cgtgcccaag tttgctgtgc
     7681 cgtaaactct gcctgcaaat aggtctcgtc ataggcttct gtttcatatg cctgagtgac
     7741 tgtatcggcg cgaggacgag gatttcggaa gaggagcccg caatccatgc aggcaacgat
     7801 ggcggttgca taatcctggg tgaagtttac gcgatccgtg gcagtggcag cgttttgttg
     7861 ggaccagcga cagcggtaga agcgttcgag aaacctctgc tgcgagagaa catcttcccg
     7921 agagcacacc agacggctgc gtctggaccc gcaaagcccg caccctacgt gcacccgcgg
     7981 ctgttcctcg agatcgaggt catcggtctt ggcctggggt tgagcgctgt tcattggtcc
     8041 catctccgac gcgaggagcg tcacgcgtct tcccacaact ctatgaggca gccactgggg
     8101 cgccaactgg gtagaaccca agatgaactc cagtgaaagg aacgctgcag cttcgaaaaa
     8161 aaagatcatg tgaggaaaga gtcgttcaaa tgaaaactag ggtactacct agtaggctcc
     8221 ccaaagaact cggcgtacgg tcctggcttg gctaatctgt cccatcga
//
LOCUS       DASTQY010000251        18827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_844470, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000251 DASTQY010000000
VERSION     DASTQY010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 18827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18827
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844470"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1268
                     /gene="ispG"
                     /locus_tag="VFR82_08325"
                     /gene_synonym="gcpE"
     CDS             90..1268
                     /gene="ispG"
                     /locus_tag="VFR82_08325"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEU4501643.1"
                     /translation="MHITRRLTRQIQVGKVKIGGDAPVSVQSMCSTDTRDVTATIEQI
                     QQLEAVGCEIIRVAVPDVEAAAALPRIKAAMTVPLIADIHFDHRLALKAAEIVDCVRI
                     NPGNIGAWWKVEEVIKAVNARGIPLRVGVNGGSLERPLLDKYGWPSPEALAESALNAV
                     HALEDVGFTNLKVSLKASDVHHAIDAYWLFAHQSNYPLHIGITEAGTAMTGAVKSSIG
                     LGYLLSQGIGDTLRVSLAADPVEEVKVGFEILKSLELRHRGINVIACPTCGRVEIDVV
                     RMANELEKKLSHIKTPLNVSVLGCVVNGIGEGKEADIGIAGGEGKGILFKKGKLVRKV
                     PMEELMDTLIHEVELLAKEKEAESSGEPLSASSEGWEALPAQPDRPAGKEIPVLSNKR
                     "
     gene            1417..1492
                     /locus_tag="VFR82_08330"
     tRNA            1417..1492
                     /locus_tag="VFR82_08330"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1450..1452,aa:Cys,seq:gca)
     gene            1882..2898
                     /gene="hpnH"
                     /locus_tag="VFR82_08335"
     CDS             1882..2898
                     /gene="hpnH"
                     /locus_tag="VFR82_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEU4501644.1"
                     /translation="MAVPLSQMYTVASYVLTQKFKGVKRYPLVLMLEPLFRCNLACAG
                     CGKIQYPDHVLDKRLTPEQCWAAAQECGAPIVSIPGGEPLIHPDMAKIVKGLVAQKRY
                     VYLCTNAILLERKLDEYQPSKYLTFSIHMDGLKDEHDLAVCRDGVYEVAVRAIRAALK
                     RGHRVTTNTTLFDDANPERVRAFFDEMMALGVEGMMISPGYSYQKAPDQQHFLKRART
                     RELFSKILRKPKRGWQFNQSPLFLDFLMGRRDYQCTPWGNPTYNVFGWQKPCYLLQEG
                     YAKTFRELIETTPWQDYGTNKNEKCADCMVHCGYEASAVEDTFGSLSGFARTAKLTLL
                     PTSR"
     gene            2974..3366
                     /locus_tag="VFR82_08340"
     CDS             2974..3366
                     /locus_tag="VFR82_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501645.1"
                     /translation="MTDTKDHTPAGDGSLEGRVFRPSSSAETAEAVELAFDYRGDVTL
                     ELKSGETLTGYLFNRDHVAADPWIEVFPAADPDPRRIAYRDILSIEFTGMDTANGKSW
                     ETWVSKRQSDRLAEAARVEADARTRGHL"
     gene            3646..4131
                     /locus_tag="VFR82_08345"
     CDS             3646..4131
                     /locus_tag="VFR82_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501646.1"
                     /translation="MCLAAAFPSVGSATHEADHRFTVEGFVCGSDGKAISDTDVLVKD
                     TKISYGQVVKTDGDGYYKATFHLHNENLGDALLVEVKGEQQNHRVQFDPKDLESERKV
                     QIHFGTGCVRDVNNPPTWVWWGLGGTAAIAIGAVGFSKIARTRRKQGQKRGKSQGKRK
                     H"
     gene            4305..4811
                     /locus_tag="VFR82_08350"
     CDS             4305..4811
                     /locus_tag="VFR82_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU4501647.1"
                     /translation="MTQSRTLQSLLMGLGLIMSGCGESGEGPVVPPPPAPAEYADKHM
                     PADWWGDAAKVEEGRKLFIGETNPDVNCASCHGKDGKPVKAGARDFRNGERMKLYSDS
                     VWFWRISEGVPNTKMKAWKSKLSDEDRWKLVLFERNFGLAGKKWDDGKKEWADAGNIA
                     VAQVNAAK"
     gene            4864..6567
                     /locus_tag="VFR82_08355"
     CDS             4864..6567
                     /locus_tag="VFR82_08355"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEU4501648.1"
                     /translation="MKTWQRGLLACCALLALFGGAAFAQTSGAPPVEFPYTGNRTAVW
                     IVAQLHILFAAFILGAPIFVVISEWLGYRKHDPRYDRLAKEVMKVTVILYSMTALTGG
                     LFIFVLLATYPQFTTWLINHFFLVFAVIYPLLFIGETILLYMYFYSWDAWKGDKKARH
                     IALGVLLNLIGTITLFVIDGPTSFMNTPVKAEGISPAEFLATASLWDKIYNYSWMPMN
                     LHRLVGNVTFGGFITGLVAAYMYMASKKEEDRAYYDWMGFVGNLIGVGALLFLPFMGY
                     LLAYELCDYDASICPYMMADQLSMFFEMQGAMVGLLFLASNYYIWLSMKRIEGVERVR
                     MTIFAPVVMVALPFVMTWAWTMFPVPDPRSLAILVPLALLPVVLGRFIPLTVSSRTVI
                     KIGFLMVVVGDAIWLTPHGFVPTGAKLVAELELPSDWNFLALMPAKNSAAFTLVFVTV
                     VNYIIYNRAINQGTIVWGKIDFVSQFVLVFLAFTSIWTMGLMGAVRSLLRKYFHTYNL
                     LPDFTSESFTPTLSYSAWWITAITVAFYVVVSFAIFVTLRPTEAKSHAPEGTPAPAGA
                     K"
     gene            6613..7410
                     /locus_tag="VFR82_08360"
     CDS             6613..7410
                     /locus_tag="VFR82_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU4501649.1"
                     /translation="MIKKVVVGLIVGGVLVGITRALEFPTVFQWMFFAYAILGTAVFV
                     LLDAPSLKPVGGVKALGALVVFYIVLCVVYIAGASLWPQYDPEDEKGKIEKILKPKRA
                     ATEQGKAEELLARAKALDEQAKALAARIKALGGDQAPGAQAAGGAGTPPSTTGAAGGD
                     LLKIGEDQWQLQECYNCHKLRGEGGKKRGPELDNIGNLLTVDDIKQKILDPKSWMAEG
                     FEKEYEKGKMPDKYKDLMEEQDVVALASWLVTFKNTSVNTPKPIKKK"
     gene            7421..8353
                     /locus_tag="VFR82_08365"
     CDS             7421..8353
                     /locus_tag="VFR82_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEU4501650.1"
                     /translation="MQPQLKSKVRCTDREIGEVTKVIVDPLSHQISHIVVSMNGSGER
                     QVAMALVQTVTDSTVQLRNTSSEIVALPPFNRAEYVSTHEVEIAHLEDKLHVTPGEVL
                     VPFPELEKNVKRRTFFMNFTHAIGFLIGLPIAFPILRYLMKPMYAPFDNAWLKIGNVG
                     KIKSDDIGVQFKYKKKVKEAYLPEAEVEKNIWILKATPAVLEKVYQGKDMEFRDATGK
                     PVWTNKKDIPYIAFSGKCPHLGCGYKWRKHKVLGQVFLCPCHLSIYDASGKVLDGPAP
                     RPLDALPIKVSASGEVQIIDMEFKAGTKSQIRIV"
     gene            8354..9466
                     /locus_tag="VFR82_08370"
     CDS             8354..9466
                     /locus_tag="VFR82_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEU4501651.1"
                     /translation="MAQSSLPSTEPTVIEKVVAFVDERIGLKQMQAKMLNEPVPGGSR
                     WAYVFGSVLLFIFIMQAVTGILLMFYYVPTADHAYASTQYIIHDVDYGWFLLSYHFWG
                     STAMVVCVFAHMSQVFLWGAYKPPREMIWLVGLALFGIVMGFGFTGYLLPWDQRAYWA
                     TTVGVEIMDKTPLVGDFMARFLKGGPTPGQMTLSRFFVIHVMILPAALMGLAGLHLFL
                     FRAAGPAGPFRGTPEEIRAKTDYFFPRQVWKDIVGMAVVFASICALAFWEPVVLLEEA
                     TPDPGEYHPEPEWYFLFLFQLLRLKVFAGEFGQFLGAIALPGAFMALLAALPFIDRDP
                     ERNIFKRPIALISWIAVMTAILLFTVSAIINREFLD"
     gene            9496..10470
                     /locus_tag="VFR82_08375"
     CDS             9496..10470
                     /locus_tag="VFR82_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501652.1"
                     /translation="MSDDSLSPTKIGLGILWSAFWTALPIKLAFAIVFMAMDLMHLEN
                     RLGVAFLMLLASPVTVFALPIISMGLESHIGEGAGIAILFLLAIPVDIWALGLVGRTY
                     FLEKFRKEPPDGLGLTLWWKSAAVGAIVLPIIWFLVSTVTSTAISMAHSLLDMEIFKG
                     IPVAERISVELTLWGSVSAIVLLVLLLIGVSLVGRIIRGTAEAARPVAENYQSIVTRW
                     DLMRVPADQGLTLTALTLGGVALSVLFWASLPVTTPHPHECCKKPDVKAEPPFKPVEA
                     LNKDEKMMAQLELQVSALEKQMAQAEKDKGKGKPKAAKEAAPPAPKKP"
     gene            10499..11371
                     /locus_tag="VFR82_08380"
     CDS             10499..11371
                     /locus_tag="VFR82_08380"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEU4501653.1"
                     /translation="MRHQGGHLQRLLRALRRQGGWLPAWVFAAGLLAVPALGLAAEGT
                     SAGPAEYRDIPGIGSRNIVWVIAQLHLLLAGFVLGVPIFAWLCEIVGWKSGEKRYDKL
                     AKEFTKLLTSSYATTALFGGILLFLLIGFYPKLMNYLTDIFFPSFVFYCILFLLETAT
                     LYLYWYGWDAMQDGGKKTFHIFLGFLLNLFAFFIMIVPNSWATFQASPVVIAEGTDIQ
                     RAWAATWNPTWWPVNIHRLIANVVLGGYICGAYAGIRYLAARNAEERDHYDWMGYVGN
                     FIGVFGLLPLPFAG"
     gene            11378..12352
                     /locus_tag="VFR82_08385"
     CDS             11378..12352
                     /locus_tag="VFR82_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501654.1"
                     /translation="MREIYQYNQQMGITLMGGFLSWLFILQAMLIGVLFLGSNYYFWL
                     GITHRIPGSEGQYKKPIMTMLVILLLCLGVWMTPHSLVASLEEARKMGGTHHPLLGVF
                     GVMSAKMTVANLMILVTFMSFIMYWRAGKQETATWAKVARSVMGAVLVLAGIAVIILG
                     VWGYFVPAIIRINYFSTSQVLIVLFVMLTITPLTALLLKSARTTTEMVWGRMPPRAGY
                     ALVLNAVMVILLMTLMGYARSSSRVHWHIYGVMRDSSQYAFSPALGYAAAFMALNTFL
                     FCMLVAFIFWVATMGDKGKAGHGAPKGELPHGMPAMAGGAPHHMDQRS"
     gene            12393..13016
                     /locus_tag="VFR82_08390"
     CDS             12393..13016
                     /locus_tag="VFR82_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU4501655.1"
                     /translation="MSEVVVLQLIGLTVVGMGIVILLFVRAQFARVIGFVAIVLGLFA
                     LVALAVPQMASLPPAEEKFDIATVKTPTDMAAIGQKIFFSKGQCALCHSIGPSESARC
                     PDLKGIGSKLSREFIFESLTQPQAYIYLDYRHEGPPKEYPARMPYINKNPIGLTKNEI
                     LSVIAFLQQMSGEPISVSPSELEVPGQAPKAPVKAEDAAPVAVAHAR"
     gene            13027..13743
                     /locus_tag="VFR82_08395"
     CDS             13027..13743
                     /locus_tag="VFR82_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU4501656.1"
                     /translation="MGSLTKPLILVVVIYLLLKFVVPNIPGSAPLPSSLIFLYLMLTV
                     AGIVIYYTMSGESKDAFWGPIQRFLTGENIGALQTARYAVLILFPLLVGWQTYGSTAV
                     SDAPPPENRTIHPAPPGEYTGLSNPVAKTPDNIMQGKGFYAAFCSPCHGGNFDGKGPA
                     ARGFNPPPANFSDPTTIAMLQESYLFWRIKKGGVGLPIEGMPWKSAMPRWEVELPDEW
                     IWKIILGEYDGAHQSPRTWE"
     gene            13767..14594
                     /locus_tag="VFR82_08400"
     CDS             13767..14594
                     /locus_tag="VFR82_08400"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HEU4501657.1"
                     /translation="MIRVSQRHSTIAAAGVALALLVAVCAPAYAQESVAVRAVMTKDP
                     MPLDDPNAAVWSSAPQAQFPMSPQVHWQNRIQEVTVKDLKVRALNDGKQVAVLLEYAD
                     PTEDPADAAALEFMVGDTKAHFAHGQPMAQVEGGPVNIWFWKNKDGKGVDMSAKGFGT
                     LKAQSHQDVTAKGIYANGTWKVVFSRPLTTEHPNEDMQLKPGEFINIAFAVWDGRKDA
                     AGDLVEKGSQKAVSSWWYFRAEAPPDYSGYIYAALAMGLALAFQFVLIRKLKKGQST"
     gene            14591..15616
                     /locus_tag="VFR82_08405"
     CDS             14591..15616
                     /locus_tag="VFR82_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU4501658.1"
                     /translation="MKAANLRVLGVVLGIAGIWGLLAGGCASEQEKRGKELYAHYCMH
                     CHGENGRQNEGFNWSSMPDPKPKDLSNKSEMSTFKDEDVFNTISRDMKDTSPGGDKIG
                     DDDFAVPTMPTFKYTLSEDEIWAIVGYVRTLHGMKLEFNVEERKKQLADAVKTAQEKF
                     EQAKQAYEAAEKRASEEAEKKSAALKKDVDVDESAYAKEQAAMGQAKKELDAAQATLA
                     YFSTRPGKGLNVARPDLTLKPEQSTALAEVGKRLYANKYGCNGCHSLAGEGGKVGPAL
                     DRAGFRLNGTWIYRWVKNPQGMKPETRMPALGLSDADAKAVTMYLATLRAPRAEEPVP
                     ATAKPAS"
     gene            complement(15666..16544)
                     /gene="ubiA"
                     /locus_tag="VFR82_08410"
     CDS             complement(15666..16544)
                     /gene="ubiA"
                     /locus_tag="VFR82_08410"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR01474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEU4501659.1"
                     /translation="MSSAESGPQLTWSNVSRLIRLPNQTGTWLLLLPTLWALVLAHGG
                     WPPWQLLVIFILGSFLMRSAGVVVNDLADRPFDRHVARTRERPLASGELTAGHALVVL
                     ATLLILAALLVLQLNPLTIRLSPIALLLAALYPFAKRVVHLPQAMLGIAFGWGTIMAW
                     TASRGTIDSAAWLLFSATICWAVGYDTIYALQDVEDDRRIGVKSSALYFRDRTWLAVA
                     CAFAMMLALLGIAGSIAGADWSFYGVLGVVGLWCGKQVNEIRTPILPARAFKLFQEHT
                     WIGSAILMGFFLGFVR"
     gene            16634..18568
                     /locus_tag="VFR82_08415"
     CDS             16634..18568
                     /locus_tag="VFR82_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501660.1"
                     /translation="MIVRPFFSRLSGRRRRTLRGCIGLLTLSLTGCGLFSSRDGAQIG
                     YYLPLTVQLRQAPSVPTAQFTYQDACGQTQTVSFGKPLADAIARKSGRVFQKVVTEGS
                     AVSSPIDGYEDVSVGMTTFDLYIPGKATKSYPATLAIGLDFAYTAADGNVLFSKKLQS
                     VGQGDVEVKATSCDVKGLEKIVQEAISKVTDGMAENLGMSVKIREAASAGHAGSVGPS
                     ASPPSPLVQAVPVVPAVVQAPAQPEAVATAVTNEPSVLVFRAIIRDENRNQLLHQGEN
                     VSVEIEVKNEGPGTAPGVEILVSGSPALVEMIPAVLAVGDLAAGDVKRLSVNGKVGNV
                     PEAVQGELVFALRAKSATVRLPSVKKFIVAVKPADAPDAVMVPVDVDDLAKIPGKLRQ
                     PKAVGIAIGIGQFRESGLARVKYAHQDAEMMAKYWSAVGGIPGERIRRLFDSRALKSD
                     LAETFEEWLPKQVDPTTVVYVFIAGRGLVDPATGAVLLIPFDGAGTSSARLFSLRRLQ
                     EALSKLPSQRAIVILDLSLEHLPSENGATKAAPLWPEEERGKERIMWMVGNRAIQDAH
                     EFDLGQHGLFTYQLLRGLGGSADVDRDGTILAGELCTYTKGQVLKMAREQFGNEQEPL
                     CVPGPGQGAIVRLQPVAKLK"
     gene            18686..18761
                     /locus_tag="VFR82_08420"
     tRNA            18686..18761
                     /locus_tag="VFR82_08420"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18719..18721,aa:Thr,seq:cgt)
BASE COUNT         3968 a         5243 c         5659 g         3957 t
ORIGIN      
        1 agtgaagacg tgaaccgtta accgtgaatt ccaagaaaga catattgcgg ttcacgactc
       61 acgattaacg actaacgact aacgagccaa tgcatatcac cagacgcctc accagacaga
      121 tacaagtcgg caaagtgaag atcggcggcg acgcgcccgt ctccgtccag tcgatgtgtt
      181 cgaccgatac gagagatgtg acggcgacga tcgagcagat ccagcagctc gaggccgtcg
      241 gttgcgaaat aattcgagtg gcggtgccgg atgtggaagc cgccgcggcc cttccccgca
      301 tcaaagcggc gatgacggtg ccgctcatcg cggacatcca tttcgaccat cgcttggcct
      361 tgaaggccgc tgaaatcgtc gattgtgtcc gcatcaaccc cggcaatatc ggcgcgtggt
      421 ggaaggtaga ggaggtcatt aaggcggtga atgcacgtgg catcccgctg cgggtcggtg
      481 taaacggcgg atcgctcgag cgccctctgc tcgacaaata tggctggccc tcccctgaag
      541 cgcttgccga atcggcgctc aatgcggtgc acgcattgga agatgtaggt tttaccaatc
      601 taaaagtgtc tctcaaggcg tctgacgtgc atcacgcgat cgatgcctac tggcttttcg
      661 cccatcaatc caattatccg ttgcatatcg gcatcacgga ggccgggacg gcgatgacgg
      721 gtgcggtgaa atcgtctatc ggccttggct acctgctttc gcagggaatc ggcgacacgc
      781 tgcgtgtgtc gttggcggca gatccggtgg aagaagtgaa ggtcggattt gaaattctca
      841 aatcgctgga gctgcgccat cgcggcatca atgtgattgc ctgcccgacc tgcggccgcg
      901 tcgaaatcga tgtcgtgcga atggccaatg agttggagaa gaagctgagt cacattaaga
      961 cgccactgaa tgtctccgtg ctcgggtgcg tcgtcaacgg catcggtgaa ggcaaagaag
     1021 ccgatatcgg cattgcgggt ggtgagggca aaggcattct gttcaagaag ggcaagctcg
     1081 tccggaaagt gcccatggaa gaattaatgg acactctcat ccacgaggtg gaactgctcg
     1141 ccaaagagaa agaagctgaa agctccggag agccgctctc cgcctcgtcc gaaggttggg
     1201 aagcgttgcc cgcacagcct gaccggccag caggaaaaga gatccctgtt ctgtccaaca
     1261 agcgctgacg acttgtggta attgcgtagg tagaggctct agacggcgtc tatggcgtca
     1321 atgtggatcc ggcgggagcc gccacgaggc cgtaagtctc gggcgtgtcc gctctagcca
     1381 cccatcccat cgccagacta taatgatttc ccccggggcg ccgtacccaa gtggctaagg
     1441 gagcagtctg caaaactgcg atgcagcggt tcgaacccgc tcggcgcctc caaaccgcac
     1501 ttcgtgcgtg ccggcggctc ttcaggcgca gccttcttca ttgagccgac ggatgacacc
     1561 gcaagtgttg cttgtttgta tgtgtgcagt taaatgacag agcttgctcg ttccgctgcg
     1621 ttcgatcaaa tcgcagcgga gagggtactt tgccacaagt aggctctaaa tcttcaattg
     1681 gtgcgtgccg gcggctcttc aggcgcagcc tccttcattg agccgacgga taacactgcg
     1741 ggcgtctgtc tttgaggacg ccatcttcga aaaattgacg acccgatgat ggagtgttag
     1801 aatccgcagc gtttacaatc aaccagccca ccggcggctc gccgtcgtga ccgttcctat
     1861 ctaaagcgtt ggagttcagt catggctgtt ccgctttcgc aaatgtacac ggttgccagc
     1921 tatgtcctca cccagaaatt caaaggggtg aagcgatatc ccttggtcct catgctggag
     1981 cctctcttcc gttgcaattt ggcctgcgcc ggatgcggca agatccaata ccccgaccac
     2041 gtcctcgata aacgtctgac acccgaacaa tgttgggcgg cagcccagga atgcggcgca
     2101 ccgatcgtca gtattcccgg cggcgagccg ttgatccatc ccgatatggc caagatcgtc
     2161 aagggcctcg tcgcccagaa acgctatgtc tatctctgta ccaatgcgat tttgctcgag
     2221 cgcaagctcg atgagtatca gccctccaag tatctcacgt tcagtattca catggacggc
     2281 ttgaaagacg aacatgatct cgccgtgtgt agagatggcg tgtatgaagt cgccgtgcgg
     2341 gcgatccgag cggcactcaa gcgcgggcac cgggtcacga ccaacaccac cttattcgac
     2401 gacgcgaatc cggagcgtgt gagagcgttc ttcgacgaaa tgatggcgtt gggtgtcgaa
     2461 ggcatgatga tctcgcccgg gtacagctac caaaaggcgc cagaccagca acacttcctc
     2521 aagcgcgcca ggacgagaga gcttttctcg aagattctcc gtaagcccaa gcggggttgg
     2581 cagttcaacc aatcgccgct ctttctggat ttcctgatgg gacgacggga ctatcaatgc
     2641 acgccgtggg ggaaccctac ctataacgta tttggatggc aaaagccgtg ctatctcctg
     2701 caggagggct acgccaagac gttccgtgaa ctgatcgaga ccaccccgtg gcaggactac
     2761 ggcacgaaca agaacgagaa atgcgctgac tgcatggtgc attgcggcta tgaggcgtcg
     2821 gccgtcgagg atacatttgg aagcctgtca ggcttcgcgc gaacggccaa gttgaccctc
     2881 ctgcccactt cccgataacc ctcgagcagc agcgttcaac tttcgaacat cgtgctttta
     2941 tggcaacgct tcacgggcgg agaggtaaga gctatgaccg atacaaaaga tcacactccg
     3001 gcaggcgacg gttcactgga aggacgggtt tttcgaccat cgtcctctgc cgaaactgcc
     3061 gaagccgtgg aactggcgtt tgattacagg ggcgatgtga cgcttgagct caaatccggt
     3121 gagacgctga cgggctattt gtttaatcgc gaccatgtgg ctgcagatcc gtggatcgaa
     3181 gttttccccg cggccgatcc tgacccccgc cgaattgcct atcgcgatat tctgtcaatt
     3241 gaatttacgg gcatggacac cgccaatggt aaatcgtggg agacctgggt ctccaagaga
     3301 cagtccgacc gactggcaga agccgcacga gtagaagcag acgctcgcac ccggggccat
     3361 ctatagcacg gagatgccgg tcccactgag cacgatttct cccgcgtctc ctctttctct
     3421 ccgcacttat cctggcgcat ctcaaggctt gcgtcgtccg tccggccggg cgctctcgtt
     3481 gacaaccagg aggccctatg ctagaaacat cggcctcaaa actggggaag cctggcgtgg
     3541 tgaggcgttt tgttgtttga aaccattgcg cgagcggcag gaacgcgttc cagtttgagg
     3601 gatgcagggc caggacggag aagactgagg gtcgtagcgg tcgtcgtgtg tctggctgct
     3661 gcgtttccat cggtcggttc ggcgacgcat gaagccgatc atcgttttac cgtcgaagga
     3721 ttcgtctgtg gctctgacgg aaaggcgatt tccgataccg acgtccttgt caaagacacc
     3781 aagatctctt atggccaggt cgtgaagacc gacggagacg ggtactacaa ggcgacgttc
     3841 catctgcaca acgaaaatct cggagatgcg ctcctggttg aggtcaaggg tgaacagcag
     3901 aatcataggg tccagttcga cccgaaggac ctggagtctg agcgtaaagt tcaaattcat
     3961 tttggaaccg gctgtgtccg cgatgtgaac aacccgccta cgtgggtgtg gtggggatta
     4021 ggaggaacgg cagccatcgc gataggggcc gtcgggttca gcaaaatcgc aaggacacgg
     4081 cgcaaacagg ggcagaaacg aggcaagtcg cagggcaaaa gaaagcatta acaccgatgg
     4141 cggtccgtgg ggctgatggc acctcgccgg atcggtcaag gatgtggcga gggtccgtgc
     4201 gcgtagcttc cgggatcttg aggtatcggc gctttgctgg agtccaggca ctcccgttaa
     4261 agcgtttttt ttcgtctgaa atcatataag gagtgaaaac cgttatgacg caatcgagaa
     4321 cgctgcagtc attgctaatg gggctggggt tgatcatgag cgggtgtggg gaaagcggcg
     4381 agggaccggt ggttcctccc cccccggcgc ctgcggaata tgccgataag cacatgcccg
     4441 ccgactggtg gggggatgcg gccaaggtgg aagaaggccg caagctgttc attggtgaaa
     4501 cgaatcctga cgtgaactgt gccagctgtc atggcaagga tggcaagcct gtcaaggccg
     4561 gagcccgcga tttccgcaat ggagagcgca tgaaactcta ctcggattcc gtgtggttct
     4621 ggcggatttc cgaaggcgta cccaatacca agatgaaggc ctggaagagc aagctctcgg
     4681 atgaagaccg ctggaagttg gtcctgttcg agcgcaattt cggattggcc gggaaaaaat
     4741 gggacgacgg aaaaaaggaa tgggcggacg caggcaacat tgcagtggcc caagtcaacg
     4801 cggccaagta acggctcgac tgcattatcc taaccggatt cgtggaggag ggcggcgaga
     4861 cttatgaaaa catggcagcg cggtttgttg gcttgctgtg cgcttctggc gctgttcggg
     4921 ggagcggcct ttgcgcagac ttctggcgcg ccgccggttg agtttccgta tacgggcaac
     4981 cggactgcgg tctggatcgt tgctcaactc cacatcttgt ttgccgcatt catcctcggc
     5041 gcaccgatct tcgtcgtcat ttccgaatgg ctcggatatc ggaagcatga tcctcgctac
     5101 gaccgcctgg ccaaggaggt catgaaggtt acggtcattc tttacagcat gaccgccctg
     5161 accggtggct tattcatctt cgtcttgctg gcgacctacc cgcagttcac gacctggctg
     5221 atcaatcact tctttctggt attcgcagtc atttatccgc tcctcttcat cggagagacc
     5281 atccttctct acatgtattt ctattcgtgg gatgcctgga aaggtgacaa gaaggccagg
     5341 catatcgcgc ttggcgtcct tctgaatttg atcggcacga ttactctgtt cgtgatcgac
     5401 ggtccgacgt cattcatgaa cacgccggtc aaggccgaag gcatttcgcc cgcagagttt
     5461 ctcgctacgg caagcctctg ggacaagatc tataactaca gttggatgcc gatgaatttg
     5521 caccggctcg tcggcaacgt gacgttcggc ggcttcatca ccggtctcgt ggcggcctat
     5581 atgtacatgg cgtcgaagaa agaagaggat cgggcatatt acgattggat gggatttgtc
     5641 ggaaatctga tcggcgtcgg cgccctcttg tttctgccct ttatgggcta tttactggcg
     5701 tacgagctgt gcgattacga cgcgtcgatt tgtccgtata tgatggcgga ccagctctca
     5761 atgtttttcg aaatgcaagg cgcaatggtc gggcttctct tcctggcgag caattactac
     5821 atctggctca gcatgaaacg gatcgaggga gtagaacggg tccggatgac catttttgcg
     5881 cccgtagtca tggtggcgct cccctttgtc atgacgtggg catggaccat gttccccgtg
     5941 ccggatcctc gttcgcttgc catcctggtc ccactggctc tcttgccggt cgtgctcggc
     6001 cggttcattc cgctgaccgt gtcgtcccga accgtcatca aaatcgggtt cttgatggtc
     6061 gtggtcggcg acgccatctg gttgaccccc cacggattcg ttcccacggg cgctaagctg
     6121 gtggctgagc tggaactgcc ctcggattgg aacttcctgg cgctcatgcc ggcgaagaac
     6181 tcggcggcct tcaccttggt gtttgtgacg gtcgtgaact acattatcta taaccgggcc
     6241 atcaatcagg gcacgatcgt ctgggggaag attgatttcg tatcacaatt cgtcctggtg
     6301 tttctcgcgt tcacgtcaat ttggacaatg ggcttgatgg gggcagtccg gtcactgtta
     6361 cgaaaatatt tccatactta caatctactg ccggatttca cctcagagtc cttcaccccg
     6421 acgttatcgt attccgcctg gtggatcacc gcgattactg tggcctttta cgtggtggtg
     6481 agcttcgcca tcttcgtcac gctgagaccg acagaggcga agagccatgc accagaaggc
     6541 actccagccc ccgctggagc caagtaagtc gggtcgaacc ttcatgcaag caaaaggatt
     6601 tctatgggga acatgatcaa gaaggtggtc gtgggattga tcgtcggcgg ggtgctcgtc
     6661 ggcatcacga gggccctgga attcccgaca gtgtttcagt ggatgttctt tgcctatgcg
     6721 atcctcggga ccgccgtgtt tgtcctcctc gatgcgccgt ctctcaagcc ggtcggcggg
     6781 gtcaaagccc ttggcgcgct cgtagtgttt tacatcgtgc tgtgcgtcgt ctacattgca
     6841 ggcgcgtctc tctggccgca gtacgatccg gaagacgaaa aaggaaagat tgaaaaaatt
     6901 ctgaagccta aacgcgcggc cacggaacag ggaaaggccg aagaattgct ggcccgggcg
     6961 aaggcgctcg atgagcaggc aaaagccctg gcagcgagga tcaaggcgtt gggcggtgat
     7021 caggcacctg gagcgcaggc cgcgggcggt gccggtaccc cgccatccac gaccggcgcc
     7081 gccggcgggg acctcctcaa gattggggaa gaccaatggc agctccagga gtgctacaac
     7141 tgccacaagc ttcgcggcga gggtgggaaa aaacgcggtc cggagctcga caatatcggc
     7201 aacctgctga ccgtcgacga catcaagcag aagatcctcg atcccaagag ctggatggcg
     7261 gaaggcttcg agaaagagta cgaaaagggc aagatgcccg acaagtacaa ggatctcatg
     7321 gaagagcagg acgtggttgc gctcgcctcg tggctggtca ctttcaagaa cacctcggtc
     7381 aacacgccga agccgatcaa gaagaagtaa gcagaggcat atgcaaccgc aactgaaatc
     7441 gaaggtccgc tgcacagacc gcgaaatcgg agaggtgacg aaggtcatcg tcgatccgct
     7501 ctcgcatcag atcagccaca ttgtcgtctc catgaacggg agtggcgagc ggcaggtcgc
     7561 gatggctctc gtacagaccg tgaccgacag tacggtgcag ctacgcaaca cctcctcgga
     7621 aatcgtggcg ctcccgccgt tcaaccgggc cgaatatgtc agtacgcacg aagtcgaaat
     7681 cgcccatttg gaagacaagc tgcatgtgac cccgggcgaa gtcctggtgc ccttccctga
     7741 attggaaaag aacgtcaagc gccgtacgtt cttcatgaat tttacccatg ccatcggatt
     7801 cttgattggg ctgccgatcg cgtttccgat cctccgttat ctcatgaagc ccatgtatgc
     7861 accgtttgac aacgcatggc ttaagattgg taacgtcggg aagatcaagt ccgatgacat
     7921 cggcgtccag ttcaaataca agaagaaggt caaagaagcg tatctgcccg aagccgaggt
     7981 cgaaaagaac atctggattt tgaaagccac ccccgcggtg ctcgagaagg tctaccaggg
     8041 caaggatatg gaattccgtg acgcgacggg gaaaccggtc tggacgaata agaaagacat
     8101 cccctatatc gccttttcgg gcaagtgccc gcatctgggc tgcgggtaca agtggcggaa
     8161 gcataaagtg ctcggccagg tctttctctg cccctgccac ttgagcattt acgatgcgtc
     8221 cggcaaggtg ctcgatggcc cggcgcctcg accgcttgac gcgctgccga tcaaagtctc
     8281 cgcaagcggt gaagttcaaa tcatcgacat ggaattcaaa gccggcacga agtcccaaat
     8341 acggattgtt taaatggcac agtcgtcgct ccctagtacc gaacccaccg tgatcgagaa
     8401 agtcgtggcc ttcgtcgacg agcgcatcgg cttgaagcag atgcaggcga agatgctcaa
     8461 cgagccggtc ccaggcggct cccgttgggc ctacgtgttt ggctccgtgc tgttgttcat
     8521 tttcatcatg caggcggtca cgggcattct cttgatgttc tattacgtgc cgaccgccga
     8581 tcacgcgtat gcgagcacgc agtacatcat tcatgacgtg gattacggct ggttcctcct
     8641 gagttaccac ttctgggggt ccaccgccat ggtcgtctgt gtctttgccc acatgtcgca
     8701 ggtctttctc tggggcgcct acaaacctcc tcgcgaaatg atctggctgg tcggcctggc
     8761 gctgttcggc atcgtcatgg ggttcgggtt caccggctac ctgcttccgt gggatcagcg
     8821 cgcctactgg gccacaaccg tcggcgtgga gatcatggac aagaccccgc tggtcgggga
     8881 tttcatggcc cgattcctca agggcggtcc gacgccgggc caaatgaccc tcagccggtt
     8941 ctttgtcatc cacgtcatga tcctgccggc tgccctcatg gggcttgcgg gccttcatct
     9001 gttcctgttc cgagcggccg gcccggccgg tccctttcgc gggacgccgg aagaaatccg
     9061 cgcgaagacc gattactttt tcccacggca agtctggaaa gacatcgtcg gaatggccgt
     9121 ggtcttcgcc agcatctgtg ccttggcctt ttgggaaccg gtcgtgctgc tggaagaagc
     9181 gacacccgac cctggcgaat accatccgga acccgaatgg tacttcttgt tcttgttcca
     9241 gttgttgcgc ttgaaagtat tcgccggtga gttcggccag ttccttggcg ccatcgcgtt
     9301 gcccggcgca tttatggccc tgctggccgc gttgccgttt atcgatcgcg atcccgaacg
     9361 gaacatcttc aagcgtccga ttgccctcat tagctggatt gcggtgatga cggcgattct
     9421 tctgtttacg gtgtccgcga tcattaaccg cgaatttctg gattaagtgc ggcgcgagcc
     9481 ggccttatca atcatatgag cgacgactct ctctctccca cgaaaatcgg cctgggtatt
     9541 ctctggtccg cgttttggac ggctctgccg atcaagctgg cctttgccat cgtgttcatg
     9601 gctatggatt taatgcattt ggaaaacagg ctgggtgtgg ccttccttat gctattggcg
     9661 agtcccgtga ccgtctttgc gctgccgatc atctccatgg gtttggagtc gcacatcggc
     9721 gaaggcgcgg gcatcgccat cctttttctg ctggcgattc ccgtcgatat ctgggctctt
     9781 ggactcgtgg gtcgcaccta tttcctcgag aaattccgca aggagccgcc cgatgggctc
     9841 ggactgacgc tgtggtggaa gtccgctgct gtcggcgcga ttgtcttgcc catcatctgg
     9901 ttcctcgtca gtacggtgac ctccacggcg atttcgatgg cccattcgct tttggatatg
     9961 gagatcttca agggtattcc ggtggccgag cggattagcg tcgagctgac cctgtggggg
    10021 agcgtgtcgg ccattgtttt gctcgtgctg ttgctcatcg gcgtgtcgct cgtcggccgg
    10081 atcattcgcg gcaccgcaga agccgcccgc ccggtggccg aaaactatca gagcatcgtc
    10141 acacgctggg atttgatgcg tgtaccggca gatcaaggcc tgacgctgac ggcgctgacg
    10201 ctcggcggcg tggctctctc ggtcctgttt tgggcgtccc tcccggtgac cactccgcat
    10261 ccgcacgagt gctgcaagaa accggacgtc aaggccgagc cgccgttcaa gccggtagag
    10321 gccctcaaca aagatgaaaa gatgatggca cagttggaac ttcaagtaag cgcattggag
    10381 aagcagatgg cccaagccga gaaggacaaa ggcaagggaa agccgaaagc cgcgaaagag
    10441 gccgctcctc ctgcgcccaa gaaaccgtaa tcgtgcttca caggatgagg tgagtaccat
    10501 gcgacaccag ggcggacatc tacagcgatt gctccgggct cttcgccgtc aggggggatg
    10561 gcttccagcg tgggtgtttg cagcgggcct gctggccgtg cccgcactcg ggctcgcggc
    10621 ggaggggacg tcggccggtc cggcggagta tcgagatatt ccaggcatcg gtagccggaa
    10681 cattgtgtgg gtcatcgctc agctccattt gctgttggcg ggtttcgtcc tcggcgttcc
    10741 catcttcgcc tggctctgcg aaatcgtcgg ttggaaaagc ggcgagaaac ggtacgacaa
    10801 gctggccaaa gagtttacga aactgctgac ctcctcctac gcgacgaccg cactcttcgg
    10861 cggaatcctg ttgttcctgt tgatcggctt ctatcccaaa ctcatgaact atctgaccga
    10921 tatctttttc ccctccttcg tcttttactg catcctcttc ctgctcgaga cggccacgct
    10981 ctatctttat tggtatggct gggacgccat gcaggatgga gggaaaaaga cctttcacat
    11041 ttttctcggg ttcctactga atctcttcgc ctttttcatc atgatcgtgc cgaactcctg
    11101 ggcgacattc caagccagtc ctgtcgtcat tgcggaagga acggatatcc agcgagcctg
    11161 ggcggcgacc tggaatccga cctggtggcc ggtgaacatc catcgcctga ttgccaacgt
    11221 cgtcctcggc ggctacatct gcggcgccta tgccggcatc cggtatctgg ccgcccgaaa
    11281 cgccgaggaa cgggatcact acgactggat gggctatgtc ggaaacttca tcggcgtgtt
    11341 tggtctcctg ccgcttccct ttgccgggta gtggttgatg cgggagatct accagtacaa
    11401 ccagcagatg ggcatcacgc tcatgggtgg atttctctcc tggctgttca ttttgcaagc
    11461 gatgctgatc ggggtgctgt tccttggttc gaactattat ttttggctcg gcattaccca
    11521 ccggataccc ggttccgaag gacagtacaa aaaacccatc atgacgatgt tggtgatctt
    11581 gttgttatgt cttggtgtct ggatgacgcc gcactcgctg gtggcgagct tagaggaagc
    11641 ccgcaagatg ggcggcacgc accatccgct gctcggggtg ttcggcgtca tgtccgccaa
    11701 gatgaccgtg gccaacctga tgatcctggt gacgttcatg agcttcatca tgtattggcg
    11761 ggccggcaag caggaaacgg cgacctgggc caaggtcgcg cggtcggtca tgggggcggt
    11821 gctcgtgctg gcgggcatcg cggtgatcat ccttggcgtg tggggttact tcgtccccgc
    11881 catcatccgc atcaattact tctcgacttc ccaagtgttg atcgtcctgt ttgtgatgtt
    11941 gacgatcacg ccgctcacgg cgctgttact caagagcgcg agaacgacca ccgaaatggt
    12001 gtggggccga atgcctccgc gtgccggcta tgcgctcgtg ctcaacgcgg tcatggtgat
    12061 cctgctgatg accctgatgg ggtatgcccg ctcgtcgtcg cgcgtccact ggcatattta
    12121 cggagtcatg cgtgactcct cgcaatatgc tttttcgccg gcgctcggct atgcggcggc
    12181 cttcatggcg ctcaatacgt tcttgttttg catgctggtg gcgttcatct tctgggtcgc
    12241 cacgatggga gacaagggca aagcggggca cggcgcgccc aaaggggaat tgccacacgg
    12301 catgccggcc atggccggcg gggctccgca tcacatggac cagcggtcat gagggtgatg
    12361 gggtcataca ccactactaa ctcggggtca ctatgagtga agtcgtcgtg ctgcagttga
    12421 tcggtctgac ggtcgtcggg atgggcatcg tcattttgct gttcgtccgg gctcagtttg
    12481 cccgggtcat cggattcgta gccatcgtgc tgggactctt cgcgctggtg gccctggccg
    12541 ttccccagat ggcatcgctg ccgcccgccg aagaaaaatt cgatatcgcg accgtcaaga
    12601 cgccgacgga catggcagcg attggtcaga aaatcttctt cagtaaggga caatgtgcgc
    12661 tctgccattc catcggcccg agcgagtcgg ccaggtgccc ggatttgaag gggattggct
    12721 ccaagctcag ccgcgaattc attttcgaaa gtctgacgca accgcaggcc tacatctatc
    12781 tggattaccg tcatgaaggg ccgcccaagg aatatcctgc tcggatgccc tacattaata
    12841 agaaccctat cggactcaca aagaacgaaa tcctgtcggt catcgccttt ctccagcaga
    12901 tgagcggtga gccgatctcg gtgagtcctt cagagcttga agtgccggga caggcgccga
    12961 aggcgccggt caaggccgag gacgctgcgc cggtcgcggt cgcacacgca cgttgaggag
    13021 gcgcccatgg gttcgttgac caaaccactc attcttgtcg tcgtcattta tctgttgttg
    13081 aagttcgtcg tgccgaacat ccccggatcg gcaccgttgc cctcgagcct gatttttctg
    13141 tacctgatgc tgacggtggc gggcatcgtc atctactaca cgatgagcgg cgaatcgaag
    13201 gacgcattct ggggaccgat ccaacgattc ctcaccggcg agaacatcgg cgcgctgcag
    13261 accgcacgct acgccgtact cattctgttt cctctcctgg tcggctggca aacctacggc
    13321 agcaccgccg tgagtgatgc tccacccccc gagaatcgca ccatccatcc ggcgccgccg
    13381 ggcgaataca caggtctctc gaatccggtc gccaaaaccc cggacaatat catgcagggc
    13441 aaagggtttt acgcagcgtt ctgctcgccc tgtcacggcg gcaatttcga cggcaagggg
    13501 cccgcggcgc gaggctttaa tccgccgcct gcaaactttt cggatccgac gaccatcgca
    13561 atgctgcagg agagctatct tttctggcgc atcaaaaagg ggggagtcgg gctcccgatc
    13621 gaaggcatgc cctggaaatc cgcgatgccg cggtgggaag tggaactgcc ggatgaatgg
    13681 atctggaaaa tcattttggg cgagtacgac ggggcccatc aatccccgcg cacctgggag
    13741 taaacctccg tttgaatgag gcacgtatga tacgggtgag ccagcgacat tcaacaattg
    13801 cggcggcggg cgtagcgctt gcgttgcttg tggccgtctg cgcgccggca tatgcccagg
    13861 aaagcgtggc cgttcgcgct gtgatgacca aagacccgat gccgctggac gatccgaacg
    13921 ccgccgtgtg gagcagcgcc cctcaggccc agttcccgat gtcgcctcaa gtccattggc
    13981 agaatcgcat tcaggaagtc accgtcaagg atttgaaagt gcgcgcgttg aatgacggca
    14041 agcaggtagc cgtcctcctg gaatatgcgg acccgaccga agatccggcc gacgccgcag
    14101 cactcgagtt catggtcggg gacacaaagg cgcattttgc ccatgggcag ccgatggccc
    14161 aggtcgaagg cgggccggtg aacatctggt tttggaaaaa caaggatggc aaaggcgtcg
    14221 acatgagtgc caagggcttc ggcacgctca aggcgcaaag ccatcaagac gtgacggcca
    14281 agggcattta tgcgaacggg acctggaagg tcgtgttttc ccgtccgctg accaccgagc
    14341 accccaatga agacatgcag ttgaagccgg gcgaattcat caacatcgcg tttgccgtgt
    14401 gggacggccg caaagatgct gcgggcgatt tggtggaaaa agggtcgcag aaagcggtgt
    14461 cctcctggtg gtatttccgc gctgaagcac ctccggacta ttccgggtac atctatgccg
    14521 cgctggccat gggcctcgcg ctggcctttc agttcgtgtt gattcgcaaa ctgaagaaag
    14581 ggcagtcaac atgaaggccg caaacctccg ggtactggga gtggtgttgg gcattgccgg
    14641 gatctggggt ctgttggccg gcgggtgcgc cagcgagcag gaaaagcggg gcaaggaact
    14701 gtacgcccat tattgcatgc actgtcacgg cgagaatggc cgccagaacg aaggattcaa
    14761 ctggtcttcg atgcccgatc ccaaaccgaa ggacttgtcc aacaaatccg agatgagcac
    14821 cttcaaagac gaagacgtgt tcaacacgat ctcacgcgac atgaaggata cgagtccagg
    14881 cggagacaag atcggtgacg atgattttgc cgttcccacg atgccgacgt tcaagtatac
    14941 cctctcggag gatgaaatct gggcgatcgt cggctacgtg cgcacgctgc acggcatgaa
    15001 gctggaattc aacgttgagg aacgcaagaa gcaactggcc gatgccgtca agacggccca
    15061 ggaaaagttc gagcaagcca agcaagccta tgaagcagct gagaagcgag ccagcgagga
    15121 ggcggaaaag aagagtgccg cgctgaaaaa agatgtggac gtggacgaat ccgcctatgc
    15181 gaaggaacag gcggccatgg gccaggcgaa gaaagaactc gatgccgctc aggcgacgct
    15241 cgcatatttt tcgacgaggc ctggcaaggg tctcaacgtg gcaaggcctg atctgaccct
    15301 gaaaccggag cagtcgaccg ctttggcgga agtcggcaaa cgcctgtatg cgaacaagta
    15361 cggttgcaac gggtgtcaca gcctggcggg agagggcgga aaggtgggac cggcgctgga
    15421 tcgagctggc ttccgtctca atggcacgtg gatttatcgt tgggtgaaga acccccaggg
    15481 catgaagccc gaaaccagaa tgccggcatt gggcttgagc gatgccgatg cgaaggccgt
    15541 gacgatgtac ctggcaacgc tcagggcgcc aagggccgag gagccggtgc ccgcgaccgc
    15601 gaagccggcg agctgagtcg cgaccaccga gcgtggcgta ttcgttcgtt cgacctgtca
    15661 ttccttcagc ggacaaatcc caggaagaag cccatgagga tcgccgatcc gatccacgtg
    15721 tgttcctgaa acagtttgaa tgcgcgcgca gggaggatgg gcgttctgat ttcgttgacc
    15781 tgcttgccgc accacaggcc cacgacaccg agtacgccgt agaagctcca atcggccccc
    15841 gcgatcgatc cggcgattcc gagcagagcg agcatcatgg cgaatgcgca tgccacggcc
    15901 agccaggtcc gatcccgaaa gtacagtgcc gacgacttca cgccaatccg ccgatcatcc
    15961 tcgacatctt gtaacgcata gatggtgtcg tagccgaccg cccagcagat cgtggcgcta
    16021 aagagcagcc aggcggcgct gtcgatcgta ccccgtgaag cagtccacgc catgatcgtt
    16081 ccccacccaa atgcgatgcc caacatggcc tggggaaggt gaacaacacg cttggcaaag
    16141 ggatagaggg ctgcaaggag aagggcaatg ggacttaatc gaatagtcag gggattgagc
    16201 tgcagtacta agagcgcagc gagaatcagg agagtggcca ggaccaccag cgcgtgtcct
    16261 gctgtgagct cgcccgaggc caacgggcgc tcgcgcgtcc tcgcgacgtg acgatcgaac
    16321 ggccgatccg ccaagtcatt caccaccacg ccggcactcc gcatgaggaa tgacccgagg
    16381 atgaagatga cgagcagctg ccagggaggc catcccccgt gtgcgagaac gagagcccag
    16441 agcgtcggta acagcagcag ccatgtgcct gtttgattgg gcaatcgaat cagccggctg
    16501 acattggacc aggtgagctg cggcccactt tcagcgctcg acatgaaggg aacctagccg
    16561 accgagctgg cactgtcaat tcgacctacg ggaggcctct gttgtgtcgc accgagtgat
    16621 ttcggtataa gggatgattg tgcggccatt tttttcacgc ctttccggcc gtcgacgccg
    16681 gacactccgg ggatgcattg gtctcctgac gctctcgctg actgggtgcg gcctcttcag
    16741 cagtcgtgac ggcgctcaga tcggctatta tctgccgctg acggttcagc tcaggcaagc
    16801 accgagcgtg cctaccgctc aattcaccta tcaggatgcg tgcggtcaga ctcaaaccgt
    16861 gtcattcggc aagccgctcg ccgacgcgat cgcgcgaaag tccggccggg tgttccaaaa
    16921 agtggtgacg gaggggagcg ccgtctcctc tcccatcgac ggatacgagg acgtctcggt
    16981 cggcatgacc acattcgact tgtacatccc cgggaaagcc accaagagtt atccggcgac
    17041 attggccatc ggactggatt ttgcctacac ggcggcagac ggcaacgtgc tgtttagcaa
    17101 gaagctccaa agtgtgggac agggcgatgt cgaggtgaag gcaacctctt gcgacgtgaa
    17161 gggcctggag aagatcgtcc aagaagccat ctcgaaagtc accgatggca tggcggaaaa
    17221 tctggggatg tcggtgaaaa ttcgcgaagc tgccagcgcc gggcatgccg gatcggtcgg
    17281 tccgagcgca tctccgccgt cgcctcttgt ccaggccgtt cctgtcgttc cagcggtagt
    17341 ccaggctccg gctcagccag aagctgtggc cacggccgta acgaatgagc cgtcggtgct
    17401 ggtatttcgt gccatcatcc gtgatgaaaa tcgcaatcag ctccttcatc agggcgaaaa
    17461 cgtgtcggtc gaaatcgagg tgaagaacga aggccccggc accgcccctg gagtggagat
    17521 tctagtcagc gggagtcctg cgctggttga gatgatcccg gcagtactcg cagtgggcga
    17581 cctcgccgcc ggagacgtca aacggctgtc agtaaacgga aaggtcggca acgtaccgga
    17641 ggcggtccaa ggcgaactcg tatttgcatt gcgtgcaaag tctgcgacgg tgcgattgcc
    17701 atcggtaaaa aaattcattg tggctgtcaa gcccgccgac gctccggacg cggtcatggt
    17761 cccggttgat gtcgacgatc tggcgaagat cccaggcaag ctcaggcagc cgaaagcagt
    17821 aggcatcgcg atcggcattg ggcaattccg cgaaagcggc ctcgcgcggg tgaaatatgc
    17881 gcatcaagat gcggaaatga tggcgaaata ttggagtgcc gtcgggggaa tcccgggtga
    17941 gaggatacgc cggttgttcg acagcagggc gctcaaaagc gacctcgctg aaacatttga
    18001 ggaatggctg cccaaacagg tcgatcccac gacggtcgtc tacgtgttca tcgccggtcg
    18061 cggtctcgtg gatccagcca ctggagccgt gttgttgatc ccgttcgatg gggctggtac
    18121 ttcttcggcc aggctctttt cactccggcg gttgcaagag gcattgagca aattgccgtc
    18181 tcagcgtgcg atcgtcatcc tcgatctctc actggagcat ctgcccagcg agaacggggc
    18241 aacaaaggct gcgccacttt ggccggaaga agagcggggt aaggagcgta ttatgtggat
    18301 ggtcggcaat cgcgccatcc aggacgccca tgagttcgac ctcggccaac acggcctgtt
    18361 cacctatcag ttgctccgag ggcttggtgg gtcggcggac gtggacagag atgggacgat
    18421 tctcgctgga gaactctgca cctacacgaa aggacaagtc ctcaaaatgg cccgtgaaca
    18481 gttcggcaac gagcaggagc ctctctgcgt gcccggtcca gggcagggag caatagtgag
    18541 actgcagccg gtagccaaat tgaaataagt tgcatctcag attccgatta atactgcaca
    18601 cccttccaaa ggtgctctca gccgagattc ctcttgagcc ctaagaaaaa cccgtgatag
    18661 ggtagggcct cttctttccg aaaaagccgg cgtagctcag ttggtagagc agcggtttcg
    18721 taaaccgcag gtcgctggtt cgattccagt cgccggctcc aggtggcgaa gccagctgga
    18781 gccttaccaa tacctaagaa ccagcgaact gaggggattc gaagggg
//
LOCUS       DASTQY010000252         6573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_915923, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000252 DASTQY010000000
VERSION     DASTQY010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915923"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2290
                     /locus_tag="VFR82_08425"
     CDS             <1..2290
                     /locus_tag="VFR82_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4501661.1"
                     /translation="KDRDGRFTLVNQAVADCYGTTVEELLGKTDADFNSNVEEVEQFR
                     RRDREVIDGLEELFIPEEVITDASGSVRWLQTVKRPILDERGQATHVLGVATDITQRR
                     LAEEALRRNEELLRLVTDSSPIAMAYCDEQQRLRFVNRAYVDRFRFEPDQVIDKPIAE
                     VIGSQAYDTFHAYIDAALRGTRSDFEAAVPYERIGTRYMRCAYVPHLDHQGRAIGFTA
                     VMMDITDRRRMEEALRESEERLRLALAAGQMGAWDVDLATDVTRWDRKEFALLGLAEG
                     STTPSTTEFYRHVHPDDLPLVEESVQRALRGSTGAMEHEFRVVRPDGSIRWLEAKGQV
                     LNDHEGRPVRMVGVNFDVTERKRTEERLRSFTHELEARVAERTHELTQLHERLRALAT
                     ELNLTEQRERKRLATDLHDYLTQLLALVCMKLSQIKRKAPAPTQADIAQTEGVVNEAL
                     VYTRTLVTQLSPPVLHEFGLPVALRWLADQMIRQELTVDVCQSVSDQVSLPEDQAVLL
                     FQSVRELLINVRKHARTNRARMTIEQTGANLCITVRDEGAGMELQAAQTSQPSATSSK
                     FGLFSIRERMLAIGGCLEIESAPGRGMTSRLILPLARPESVSQGDAGQLQTLCQSRED
                     ESLQENERESAGKDGRIRVLLVDDHTIVREELRVLLYVHEDLLVVGEAGDGEQAILLA
                     DKLRPDCVIMDVNMPRMDGMEATRRIKAAFAETAVIGISVNTSQEMMAAMRRAGADAF
                     LSKEAPAEDIHHTIVTMTRKCA"
     gene            complement(2483..2878)
                     /locus_tag="VFR82_08430"
     CDS             complement(2483..2878)
                     /locus_tag="VFR82_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501662.1"
                     /translation="MKLLPSLVLGVWTLLSHSAAESAMQEAKHFVVDIISINGEEFVV
                     KDENGKEANIHVGTETEKFGHLQVGDRIDAWIYPNGQAKTIMILRSASVIKQDQAQKN
                     QQEAQQKQQESQQRAEAPQQVQPEEGALR"
     gene            complement(2955..3122)
                     /locus_tag="VFR82_08435"
     CDS             complement(2955..3122)
                     /locus_tag="VFR82_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEU4501663.1"
                     /translation="MLSWAVTFLVVAIIAGILGLSGVAGTATQIAYVLFVVFLIFAAV
                     SFVMGRRPPVT"
     gene            complement(3208..3936)
                     /locus_tag="VFR82_08440"
     CDS             complement(3208..3936)
                     /locus_tag="VFR82_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4501664.1"
                     /translation="MHEYSFMRELTRTCLLLALLLVIGLPLPPASQAQDTLWNTWRQG
                     GLQALHEGRLADAERLLTAALEQAEKYGLDDLRVADAANDLAVVYANSGKPLEAELLF
                     SRALAIGEKGLGADHPGVGATIQNLGILYATQERYREAEPLLKRSLAINLKEFGTNHA
                     RTALSLKTLSSFYAIQGQLGEAERLIHKSLAILEESRVRQGDPQLISTLEVLAAILRN
                     TNREDEAQEIEGRLKTAQDTTVYR"
     gene            complement(3929..4144)
                     /locus_tag="VFR82_08445"
     CDS             complement(3929..4144)
                     /locus_tag="VFR82_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501665.1"
                     /translation="MCASCWRVQPLSAELGVELQEWSDPTTFMASGQVSSGKYDIVDG
                     FCDPCLIEMALRDQLALARAEGERLNA"
     gene            complement(4321..5415)
                     /locus_tag="VFR82_08450"
     CDS             complement(4321..5415)
                     /locus_tag="VFR82_08450"
                     /inference="COORDINATES: protein motif:HMM:NF018439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HEU4501666.1"
                     /translation="MGAKIGLVSFLCTMVCALCGTVPAWASDEFIAGYASAILQHEFN
                     VTDAAVEVHDGVVLVTTKTLTNIDRGKVVAAIKQVPGVTEVEIRTADPSTISQEDQAA
                     HASIPEPGAKWLPRGTLFAPIHADPRWPQFGASYRQFTEGLNLAGVFAANFGETFAIY
                     RNKAFLDGEWEFGVQAGVFSIFDVSAQSIDLVNADYRVGILSSYRNGPFSGFIRLQHQ
                     SSHLGDEFLLSNPGITRVNLSYEELDVKLSYQLFQWLRVYGGGGFIVHRFPEDLGRGT
                     TQWGAELVSTKTFLGGRLRPVAYADFQCNERTRWQVGHSIMAGVQFENARIGDRQIQL
                     LGEYYSGPSPDGQFYVQRAAWYGIGIHLYF"
     gene            complement(5482..6069)
                     /locus_tag="VFR82_08455"
     CDS             complement(5482..6069)
                     /locus_tag="VFR82_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4501667.1"
                     /translation="MRSHLPVWSGCLLGLVVWAVVVVVPAAGADGLFEQVKIGPVSIG
                     GRATYYDPKDASGEWYGGAQVRLHAFRFLAIEGSADYRRNDFGNTRVHSYPVQVSALI
                     YPFGTMRLAPFVLGGGGWYYSTVKGPGNFDDTQNRFGFHAGGGLQFFLNNHWSIDSTY
                     RYIWLEKIESKDQNVVDKKFNDNGHMITIGLNFHF"
     gene            complement(6169..6450)
                     /locus_tag="VFR82_08460"
     CDS             complement(6169..6450)
                     /locus_tag="VFR82_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEU4501668.1"
                     /translation="MLRVVISSIMFGVLAAGPAWALFESNKELVAGAKIPLEEAVKSA
                     IKAVPGKAAEADLGKDDGRTIWKIEVIDHNNKSQTVYVDAQTGMAKMNK"
BASE COUNT         1460 a         1872 c         1802 g         1439 t
ORIGIN      
        1 caaagatcgt gacggtcgct ttacgctggt gaatcaagcc gttgccgatt gctacggtac
       61 tacagtggag gaattgttag ggaaaaccga tgcggatttt aattcgaatg tcgaagaagt
      121 cgagcagttc cgacgcaggg acagagaagt catcgatggt ctcgaagagc tgttcatccc
      181 tgaggaggtg atcacggacg cctccgggag cgtgcgatgg ctccagactg tcaaacgacc
      241 tatccttgac gagcgtgggc aagcaactca tgtccttggc gtggccaccg atatcacgca
      301 gcggcggctg gctgaggagg ccttgcggcg taatgaagaa ctactccgct tggtcaccga
      361 tagttctccg atcgccatgg catactgtga tgaacaacag cggttgcgct tcgtcaatcg
      421 cgcctatgtt gaccggtttc gattcgagcc cgaccaggtc attgataagc cgattgccga
      481 ggtcatcggc tctcaagcct acgacacgtt tcatgcctac atcgatgccg cgttgcgcgg
      541 gacccgttct gatttcgaag cggccgtgcc gtatgagcgt atcggtacac gatacatgcg
      601 ctgcgcctat gtgccgcatc ttgatcacca gggccgggcc atcggtttca ccgccgtcat
      661 gatggatatc accgaccgac ggcggatgga ggaagcgttg cgcgaaagtg aggagcgctt
      721 gcgactggcc cttgcggccg ggcaaatggg tgcgtgggac gttgatctcg ctaccgacgt
      781 gacgcgatgg gatcgcaagg agttcgcctt gttgggtctg gcggagggca gcaccacacc
      841 ctcgacgacc gaattctacc ggcatgtcca cccggatgac ctgccgctgg tcgaggagtc
      901 ggttcaacgc gccttgcgcg gatcgacggg agctatggag catgagtttc gtgtggtccg
      961 accagacgga tcaatccgat ggctcgaggc aaagggtcaa gtgttgaacg accacgaggg
     1021 acgtccggtg cggatggtgg gtgtgaactt cgatgtgacg gaacgcaagc ggaccgaaga
     1081 gcggctgcgt tcattcaccc atgaactaga agccagggtt gcggagcgaa cacacgaact
     1141 cacccaactg catgaacgcc ttcgcgccct cgccacggag ttgaacctca ccgaacagcg
     1201 ggagcggaag cggctggcga cggatcttca cgactacctg acccagcttc tggctctcgt
     1261 gtgcatgaag cttagtcaga tcaagcgaaa ggcaccggca ccaactcagg cggacatcgc
     1321 tcagaccgaa ggagttgtca acgaggcgct tgtgtataca cggacgctcg tcactcaatt
     1381 gagtccgcct gtccttcatg agtttgggct acccgtggcg ctccgatggt tggccgatca
     1441 aatgatccgg caggaactta ccgtcgacgt gtgtcaatcc gtctcggacc aggtgtcact
     1501 acccgaagac caagcggtac tgttatttca atcggtacgc gagttgctga tcaatgtccg
     1561 taagcatgcc cgcaccaatc gcgcccgcat gacgatcgag caaacagggg cgaacctgtg
     1621 catcacagtc cgggatgaag gggccggcat ggagcttcag gccgcccaga cgagccagcc
     1681 ctcagccacc tcgtcgaagt ttggcctctt cagcatccgg gagcgcatgt tggcaatcgg
     1741 cggttgcctg gagatcgaat ccgctccggg ccgggggatg acgtcgaggc tcatactacc
     1801 gctggcgcga cccgagagtg tgtctcaggg agacgctggt caactccaaa cgttgtgtca
     1861 aagccgggag gacgagagcc tgcaagagaa tgagcgtgaa tccgcaggaa aggacgggcg
     1921 aatcagggtg cttctcgtcg atgaccatac cattgtgcgt gaagagttga gggtgctcct
     1981 gtacgtccat gaagacctgc tcgtcgttgg agaagccggc gacggcgaac aagccatttt
     2041 gcttgcggat aagctgcggc ctgattgcgt gatcatggat gtcaatatgc cacgcatgga
     2101 tggaatggag gcaaccagac gcatcaaagc ggcgtttgcg gaaactgccg tgatcggtat
     2161 ttctgtgaat acatcacagg agatgatggc tgcgatgcgt cgggcggggg cagatgcctt
     2221 cctctcgaag gaagcgcctg ctgaggatat tcatcacact atcgtcacga tgacgaggaa
     2281 gtgtgcatga ccttcacgtt ctaggtcatt atgaaacccg agcaagtgtg tgtcgcctac
     2341 aggactactt atggcgtctt gtccgttcaa cgaccaaaag caccgcgctg acgtatccct
     2401 cgccctgccc cctgaggaaa aaacgggacc gaatcgtata cccccatcaa gcaggccaca
     2461 agatagccga aagtcatgtc gactatctta atgcgccttc ttccggctga acctgctgag
     2521 gggcttcggc ccgttgctgc gactcctgtt gtttctgctg agcctcctgt tgattcttct
     2581 gcgcctggtc ctgcttaatc actgaggcgc tccgaagtat catgatggtc ttggcctgac
     2641 cattcgggta gatccaggca tcgatgcgat ccccgacctg caagtggccg aatttctccg
     2701 tttcggtacc gacatggata ttcgcctcct tgccattctc gtccttgacg acgaactctt
     2761 caccgttgat ggagatgatg tccacgacaa agtgtttggc ttcctgcatc gcactttccg
     2821 ccgcggagtg cgacaacaga gtccatacgc ccaggactaa actgggtaac aacttcatag
     2881 cgcctcccta cgcaacactc ctgtcgagca ttgctactcg aggaaagggg aacgtaccac
     2941 ggatcagttg tcgatcatgt taccggcggc cgacgtccca tgacgaaact taccgcagca
     3001 aaaatcaaaa acacgacgaa aagcacatag gcgatctgag tagctgttcc ggccacaccg
     3061 gataggccca agatccctgc aatgatcgcg acgactaaaa atgtgacagc ccagctgagc
     3121 atagtgaact cctttcgtca ggcaccgtga agcgtccctt gtagattgcc gttcgtaata
     3181 cagcacgaac ggcgggtacg cattggacta ccgatacacc gtagtgtctt gagcagtttt
     3241 gagccgtcct tcgatttctt gagcctcgtc ctcccgattc gtattgcgaa gaatggccgc
     3301 cagcacttcc aatgtcgaaa tcaactgcgg gtcgccctgt cgaacccgac tttcttccaa
     3361 gatcgcaagc gacttgtgta tcagccgttc ggcttctccg agctgccctt gaatggcata
     3421 gaaacttgag agggtcttca gcgacaaggc cgttcgcgca tggttggtcc cgaattcctt
     3481 gagattgatg gccagcgacc gcttcaaaag cggctcggcc tcacgatacc gttcctgagt
     3541 ggcgtataag ataccgaggt tttgaatggt tgcccccact ccggggtggt ctgcccccaa
     3601 gcctttttct cctatggcca acgcgcggct gaagagcagc tctgcttcga gcggctttcc
     3661 tgaattggca tagacgaccg ccaagtcatt cgctgcatcg gccacacgca aatcatccaa
     3721 accgtatttt tcagcttgtt ccagcgcagc cgtcaacagt cgctcagcat cggctaaccg
     3781 tccctcatgt agggcctgta accctccctg cctccaggta ttccacagcg tatcctgcgc
     3841 ctgagaggcg ggaggcaagg gtagtccaat gaccaacaac agggctaaca gtaagcacgt
     3901 ccttgtcagc tcacgcatga aggagtactc atgcattgag tcgctctcct tcggccctgg
     3961 cgagggcaag ttgatcgcgg agggccattt cgatgaggca gggatcgcaa aatccatcaa
     4021 cgatgtcata tttgcccgaa ctgacctggc cggacgccat aaacgttgtg ggatcactcc
     4081 actcctgcag ctcaacgccg agctctgctg aaaggggttg cacacgccag cacgacgcac
     4141 acatcagaat ttccatactt cacggtacac tgggtctccg gtgagcccta actaggacca
     4201 gtcccagaat tgcctggcag tccaccccag acccccagct gtttgatgct gatccaataa
     4261 gaagcaacct gatgatttag aagcgaaagt gtcgagatag gaactcgcgc gaaaaccttg
     4321 tcaaaagtag aggtggatgc cgatgccgta ccatgcagcc cgttgcacat aaaattgccc
     4381 gtccggtgag ggcccggaat aatattctcc gagaagttga atctggcggt caccaatgcg
     4441 ggcgttctca aactggacac ctgccatgat ggaatggccg acctgccacc tggtccgctc
     4501 gttacattga aagtctgcat aggcgaccgg acggaggcgt ccccccagaa acgtcttggt
     4561 actgaccagc tctgcgcccc attgagtagt gcctcgccct aagtcttccg ggaagcgatg
     4621 tacgatgaac cctccgccgc catagacccg aagccattgg aataactggt aggacagttt
     4681 cacatccaat tcttcgtaac tgagattcac tcgcgtgata cccgggttgc tcagcaagaa
     4741 ctcgtctcca agatgagaac tttgatgctg cagccggatg aatccggaaa agggtccatt
     4801 ccggtagctg ctcaagattc ctactcggta atccgcattg acgagatcga tcgactgggc
     4861 gctgacgtca aagatgctga agacaccggc ctgaacgccg aattcccatt ctccgtccag
     4921 gaaggcttta ttgcgataga tcgcaaacgt ctcgccaaaa ttcgcggcaa agacaccggc
     4981 aaggttcaga ccttctgtga actgtctgta gcttgctcca aactgaggcc agcgcgggtc
     5041 ggcgtggatt ggcgcgaata gtgtgccccg cggcagccat ttggcccccg gctctggaat
     5101 agacgcgtgg gcagcttgat cttcctgcga aatggtggac ggatcggcgg tgcggatctc
     5161 gacctctgtg actccgggaa cctgtttgat ggcggcgacg actttccctc gatcgatgtt
     5221 tgtcagagtt ttcgtcgtca ccaatacgac gccatcgtgc acttcgactg cggcgtccgt
     5281 gacgttgaac tcatgttgta aaattgccga ggcatagccg gcgatgaact cgtctgaagc
     5341 ccatgcgggg acggttccgc agagggcgca aaccatcgtg caaagaaacg ataccaaccc
     5401 gattttagca cccatgccat gctctaactc gatgtgtatc gtatcccgta tctgaacttt
     5461 cgcgagatac gcttcgtcgc ttcaaaagtg gaagttcagt ccgatcgtga tcatatgacc
     5521 gttatcatta aactttttgt cgacgacgtt ctgatctttg gactcgattt tctccagcca
     5581 gatataccga taggtgctgt caatggacca atggttgttc aaaaagaatt gcagaccgcc
     5641 tccggcgtgg aatccgaacc ggttttgcgt atcgtcaaag ttacccggtc ccttgactgt
     5701 tgaatagtac caaccgcctc caccgagaac aaaaggcgcg agtctcatcg tgccgaacgg
     5761 atagatcaac gcggagacct gtacgggata gctatgaact ctggtatttc cgaaatcgtt
     5821 tcgcctgtaa tcggcagaac cctcgatggc cagaaagcga aacgcatgca accggacctg
     5881 agcgcctcca taccactcac cgcttgcgtc cttcggatcg taatacgtcg ctctgcctcc
     5941 gatggaaacc ggtccgatct tgacttgttc gaatagtccg tccgcgcctg cggcaggaac
     6001 tacgaccacc acagcccaca cgacaaggcc caacagacat ccggaccaga cgggtagatg
     6061 tgagcgcacg gctgtcctcc ttcgtggtgc atgtccgcga tcattcattt ggccacaaaa
     6121 aaagcggacc gaacgcggtg gcccggtccg cttctatgcc gtggttcgtt acttgttcat
     6181 cttggccatg ccggtctgtg catcgacgta gaccgtttgg cttttgttgt tgtgatcaat
     6241 aacttcgatc ttccaaatcg ttcgtccgtc atccttgccc aggtcggcct ccgccgcctt
     6301 acccgggaca gctttgatgg cgctcttgac ggcttcctcc aaggggatct tggcacccgc
     6361 cacgagctct ttattggatt cgaaaagtgc ccacgccggt ccggccgcga gcactccgaa
     6421 catgatgctg gaaattacga ctcttaacat ggtgacctcc tcttcagacg agttacctga
     6481 caggctcgcg ttcaacctgt tcgggcttgt tgccctgaaa ccacttatcc gcccaacctg
     6541 aaatatcgtc cttctgggcg ccgtaacgtt ctt
//
LOCUS       DASTQY010000253         3721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_820997, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000253 DASTQY010000000
VERSION     DASTQY010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820997"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1048)
                     /locus_tag="VFR82_08465"
     CDS             complement(41..1048)
                     /locus_tag="VFR82_08465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4501669.1"
                     /translation="MTPDVTVVMSVYNGQPFLREAIESILSQTCRDFEFILIDDGSAD
                     ETAAIIASYRDPRIRMVRHGRNAGLAASLNEGFALASGRYIARMDADDVSRPERLASQ
                     VRFMDAQPSLGACGTWVEVSGEGIQQRWEYPATHNAIHARLLFDCSMAHPTVMFNRSR
                     LHKARLSYDSAYPCAQDYELWCRAVHGLTLANIPEVLLTRRLHASQVGRRDANAQQIW
                     SGKIRRRQLEQLGFSPSAEQIALHEAISTWTWPRTEWWIERAEDWLRGLRQANGTRAL
                     YPEPEFSAVVGERWMAICQSIQGRLDRRFWRSTLSDALDLGWRANFRGRAWRGLALLG
                     I"
     gene            complement(1045..1800)
                     /locus_tag="VFR82_08470"
     CDS             complement(1045..1800)
                     /locus_tag="VFR82_08470"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4501670.1"
                     /translation="MTGRFDRWLAWILRYGSDRLGLSQVESLLIDIIARRTRTLPADE
                     ALRLLFRLQAALYPTLGRKAVEYGGGTHTKHRHTRYHDFFTSRVRTGERVLDIGCGIG
                     AVAFDMAERSGAWVVAVDLSADNIEAARKRFPHSRVEYRVQDGRSASPDGPFDVVVLS
                     NILEHLPERSVFLRALHRAARPSRFLIRVPLFERDWQVPLKRELGVEWRLDTTHETEY
                     TLESFADEIREAGCRIAYQEVRWGEIWAEVHAA"
     gene            complement(1800..3008)
                     /locus_tag="VFR82_08475"
     CDS             complement(1800..3008)
                     /locus_tag="VFR82_08475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4501671.1"
                     /translation="MADPLANVCLALFLTRGMSLRAWDDIGLLDREAAPYRRLEPMLR
                     RVMFVTYGDRQDLTYAPRLGGIRVHCNRWRVSPQLYERYLTKAMPWSWRGPVVVKSNQ
                     VQGAEIALEAARRRGKPFIARCGYLPSDNMERSRGADSPEAKRAQALEHHVFSEADRV
                     VVTTTTMREKVIRRYQLRADRVRVIPNYVDTSLFAPSSDESRRPNQLLYVGRLDEEKN
                     PRALLAAIEGMDVELVMVGKGSQGEALHREASERRLRVRFLGNVPNEELPQLLNSSAA
                     LVMPSLIEGHPKALLEAMACGVPVIGTNVPGIRELLIHRVTGLLCEPCPESLRTAIKE
                     VLDHRQLGVRMGGAARSYVADRFSLERVVDMELALLDEMAMEIRTTHLLASGSEPTTL
                     VRSQGSRKLL"
     gene            complement(3001..>3721)
                     /locus_tag="VFR82_08480"
     CDS             complement(3001..>3721)
                     /locus_tag="VFR82_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4501672.1"
                     /translation="TEWLIEIVTRVGVCLGMYETDFHYTDRKTKARYVWLKYRSILEG
                     RILDVGADACHLREHLPPETHYCGIGLGAGVDQIVNLEEQLIPFPSGSFDCVLCLDVL
                     EHIENIHAVFDEVCRVTRKFAVVSLPNAWLDFYNMLRHGEYRAGQPMKFYGLPLEPPA
                     DRHKWFFSADEAKAFVTHRASLNKVRIVQMDHEAWGTEGYGWSGVVRTWARNLLLRPS
                     LNVRNLYAGTLWVVLEKESHG"
BASE COUNT          770 a         1181 c         1027 g          743 t
ORIGIN      
        1 ttccagggcg cacgccaact ggtttgaagg agccgcacct ttatatcccg agaagcgcaa
       61 gcccccgcca agcccgtcca cggaagttcg ctctccatcc cagatcgagt gcgtcgctga
      121 gtgtggaccg ccaaaatcgt cgatcgagcc tcccttggat ggactgacag atggccatcc
      181 accgttctcc gaccaccgct gaaaactccg gttctggata caaggcacga gttccgttcg
      241 cttgtctgag cccgcggagc cagtcctctg cccgctcgat ccaccattcg gttcgcggcc
      301 atgtccaggt actgatcgcc tcgtgcaggg caatctgttc ggcgctgggc gaaaatccga
      361 gctgctcaag ttggcggcgc cggattttcc ccgaccatat ctgctgagcg ttcgcgtccc
      421 gacggccaac ttgcgaagcg tgcagccggc gcgtcaataa gacctcgggt atgttggcga
      481 gcgtcaggcc atgcaccgcg cgacaccaca actcataatc ttgcgcacag ggataggcag
      541 aatcatagga caatcgcgct ttgtgcaggc ggctccgatt gaacatcacg gtcggatgag
      601 ccatcgaaca gtcgaacaag agtcgggcat ggatggcatt atgcgtggcc ggatattccc
      661 accgttgctg gatcccctct ccggaaactt ccacccacgt cccacaggcg ccaagagaag
      721 gctgtgcgtc catgaagcgg acttgggacg ccaaccgctc agggcggctc acgtcatcgg
      781 catccattcg cgcgatgtac cgccccgagg ccagggcaaa tccttcattc aacgaggcgg
      841 ccaagccggc attcctgcca tgcctgacca tgcgaattcg tggatcacga tacgaggcga
      901 taatggcagc ggtttcatcc gccgatccat cgtcaatgag gataaactcg aagtcacggc
      961 aggtctggct caaaatgctc tcgatcgcct ctctgagaaa aggttggcca ttgtacacgg
     1021 acatgacgac ggtgacatcg ggagtcatgc ggcgtgcacc tctgcccaga tctcgcccca
     1081 gcgaacttct tgatacgcaa tccggcatcc ggcttccctg atttcatcag caaaggattc
     1141 cagcgtgtat tccgtttcat gtgtggtatc cagcctccac tcgactccca gttcccgttt
     1201 gagcggtact tgccaatccc gctcgaacag tggcacccgg atgaggaatc tggaaggtcg
     1261 ggccgcacgg tgcagtgccc tcagaaacac ggaccgctcg ggcaaatgct cgagaatgtt
     1321 ggaaagcaca acgacgtcga acgggccgtc cggacttgcg ctccggccat cctggacccg
     1381 gtattcaact ctggagtgtg gaaaccgctt cctggccgct tcgatgttgt cggcgctcag
     1441 gtcgaccgcg acgacccagg cgcctgatcg ttccgccatg tcgaaggcca cagcgcctat
     1501 gccgcatccg atatctagga cccgttctcc cgtccggacg cggctggtga aaaaatcatg
     1561 gtagcgcgta tgccgatgct tcgtatgggt tccaccgccg tactccacgg ccttcctgcc
     1621 gagagtcgga tacaacgctg cctgcagccg aaacagcagc cgtagcgcct cgtccgctgg
     1681 aagggtccga gttcttcggg cgatgatgtc gatcagcagc gactccacct gtgaaaggcc
     1741 tagtcggtcg gacccgtatc tcagaatcca agccagccac ctgtcgaagc gtccggtcat
     1801 cacagcaatt tcctgctccc ttgggaacgc accaaggtcg ttggttcaga tccgctcgcc
     1861 agcagatgcg tcgttcgaat ctccatcgcc atctcatcga gcaaggccag ctccatgtct
     1921 acaacccttt ccagagagaa ccggtcggcg acgtaggatc gtgcggcgcc tcccattctg
     1981 acgcccaact gacgatggtc cagcacctcc ttgatcgccg tccgcagact ctcgggacac
     2041 ggctcgcaga gcagccctgt gacacggtgt atgagcagtt cacggatgcc cgggacattc
     2101 gttccgatga cggggacgcc gcaggccatg gcctcgagca acgctttggg atggccttcg
     2161 atcagtgagg gcatcaccaa cgcagccgac gagttgagga gttgcggtaa ttcttcgttg
     2221 ggcacattgc cgagaaagcg aactcgcagt cgccgctcgg acgcttcgcg gtgcaacgcc
     2281 tccccttgtg agcccttccc taccatgacc aactccacat ccatcccctc aatggccgcg
     2341 agcagcgccc gtggattttt ttcctcgtcc agtcggccga cgtagagcaa ttggttgggc
     2401 cgtctcgact cgtcgctgct cggcgcaaag agggacgtgt cgacataatt tgggatgaca
     2461 cgcacgcgat ccgctcggag ctggtagcgg cgaatcacct tctcgcgcat cgtggtagtc
     2521 gtgacgacga ctcgatctgc ctcggagaat acgtggtgtt ccagtgcctg cgcgcgtttc
     2581 gcttccggag aatccgctcc acgactcctc tccatgttgt ccgaaggaag gtatccgcag
     2641 cgcgcaatga acggcttccc tctgcggcga gcggcctcca aagcaatctc cgctccttgg
     2701 acctgattgc ttttcaccac caccggcccg cgccatgacc agggcatggc cttggtcaga
     2761 taccgctcat atagctgcgg cgacactcgc caacggttgc aatgcacacg aatcccgcct
     2821 agacgcgggg cataggtcag atcctgtcga tccccatagg tgacaaacat cactcgccgt
     2881 agcatcggct ccagccggcg atagggcgcc gcttcgcggt ccaacagtcc gatatcgtcc
     2941 caggctcgaa gggacattcc gcgagtcaag aacaaggcca ggcacacatt tgccaaaggg
     3001 tcagccatgc gactcctttt cgagcacgac ccagagtgtt ccggcataca ggttacgaac
     3061 gttgagcgac ggccggagca gtagatttct ggcccacgtt cgcacgacgc cgctccagcc
     3121 atagccttcg gtgccccacg cttcatgatc catttggacg atccgaacct tgttgaggga
     3181 ggcccgatgt gtgacgaagg ctttggcctc atccgcggag aaaaaccact tatgcctatc
     3241 ggccggaggc tcgagcggca gcccgtagaa cttcatcggt tgccccgcgc gatactcgcc
     3301 gtggcgcagc atgttgtaaa aatcgagcca ggcattgggg agagacacca cggcaaattt
     3361 cctggtcacc cggcagacct cgtcgaagac ggcatgaata ttctcgatgt gctcaaggac
     3421 gtccaagcac agcacgcaat cgaaagatcc gctgggaaag ggaatcaatt gctcttcgag
     3481 gttcacgatc tggtcaacac ctgcaccgag accgatgcca caatagtgtg tttcaggcgg
     3541 caagtgctct ctcaggtggc aggcatcagc cccgacgtca agaatccgcc cttctaggat
     3601 cgatcggtac ttgagccaca cataccgggc cttcgtcttc cgatcggtgt aatgaaaatc
     3661 cgtttcgtac atgccgaggc agactcctac tctcgtcaca atctcaatca gccattcggt
     3721 t
//
LOCUS       DASTQY010000254         4810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_810471, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000254 DASTQY010000000
VERSION     DASTQY010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810471"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFR82_08485"
     CDS             complement(<1..184)
                     /locus_tag="VFR82_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501673.1"
                     /translation="MPAQTMPTSIELLIIIFSALVILSILTIKLSVKLGIPSLTLFLV
                     IGMLAGSDGVGGLYFDN"
     gene            complement(194..1378)
                     /locus_tag="VFR82_08490"
     CDS             complement(194..1378)
                     /locus_tag="VFR82_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4501674.1"
                     /translation="MTTQSVDQSLHDHLAHWGLRRFSSDEAYARWQRQELSSDELSAL
                     HQHVERKRHGSPADEMAFYDLSAAPRILPVLYSQRYEYCVAIAPVVEAHLGEAKTVLD
                     FGCGPGILTTFYARRFPQAEVTGFDRSAACIAQARRKADEWGLKNLRFVCADLVEGPA
                     IGKYERVVATHALVQSEQDLGLPSISWRTFERAPDAQAQAAFEERTGLGRRLDRLAAA
                     MVPSGRLVVFEKTRQLSRRIPLQRAFAARGLKPFEPPRPIRYSLIEEVSDDGPLFVLA
                     LGKESACAWNEDPEPDEGLPFDRVGLTSRSSSPEAPLYENHWPSAQRVWEQLNDRKVL
                     KEATAEESDGRQLHVELGTAEGYRYLYCANTFDRRQLVIVEPAQAAMLHTYYQEIIQG
                     SA"
     gene            complement(1507..2871)
                     /locus_tag="VFR82_08495"
     CDS             complement(1507..2871)
                     /locus_tag="VFR82_08495"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU4501675.1"
                     /translation="MPEVPIKLYLDKLLKLCRNAVRKTSVIPGPVKDRALRAMAERVA
                     EDEAAILSANERDVDALGKSFGSEVSKDRLKAAVARVRMTADDVKEMAERLRAIADQP
                     DPVGAVTGRWERPDGLQVSRVRVSIGVIGIISELPPLVTVESIALCLKSGNVSVFRGA
                     PEWSQTHEVIGKGLREAAGQHGIPPDSWVFIDRPEKDVAVELIRSGRALDAIIPRGGT
                     GLRKAVLEGARIPVLCHDGGQTYLFVDADVDLPLVQNVVINSKVQRPAATNALDTLLV
                     QQGVARQFLPALINRLLVEFKIDVRGCPKTLALMGQMPISGHTSIIPAAEEDWSRQFQ
                     SPTLAVKMVADMDEALTHIAEHGLCMTAVIATSNYSSALRFTREVDASAVFVNASSRL
                     HDAEGFGFGSDIGLSSARLHAKGPIGLEQLTCEKYVVFGNGQLRMPHPVPETYFDAIM
                     LKRP"
     gene            complement(2957..4240)
                     /locus_tag="VFR82_08500"
     CDS             complement(2957..4240)
                     /locus_tag="VFR82_08500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4501676.1"
                     /translation="MARRSRTTIASLTGAESEELVPLCWSFLYFFCLLCGYYMLRPVR
                     DEMAIQGGVQHLPWMMTATFVTLLTATPFFGYLSARLPRERLLLAVYAFFGSHLIGFF
                     LIMNSHVAPQWIARLFFVWLSVFNLFVVSVFWSFMADIFTPDQGSRLFSVIAAGGSTG
                     AVVGPLLTTVLTYAVPIPVLMLVSTGFLAICWICIRALDGWSKQRPAQDIDRGGDAIG
                     GGTWAGMRLVLSSPYLLGICGYLFCLTTTATFLYLEQTRLIGEQVVSPQGRTRLFAVM
                     DLAVNVLTFATQFFITGPMIRRFGLASALVVLPAVSIAGFALVGVMPLLLVFILFTVV
                     RRVGEYAIAKPTREVLFTVLTREEKYKAKNFIDTAVSRGGDAATGWMVSMVKTLGVGV
                     TQMALVLVPLAGLWAWLGWSLGKQEEDIRIARPKG"
     gene            4335..>4810
                     /locus_tag="VFR82_08505"
     CDS             4335..>4810
                     /locus_tag="VFR82_08505"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEU4501677.1"
                     /translation="MRIIDLRSDTVTKPTEGMREAMARAEVGDDVFGDDPTVNALQQR
                     IAALLGKDAALFVPSGTQSNLVATMTHCRRGDEAIVGEMAHLYRWEAGGAGVLGSVVQ
                     LPLPHQADGSLALDDIRAAIKPDDPHFAVTRLLCLENTLGGKAVPLAWTAQATALAR"
BASE COUNT         1047 a         1488 c         1385 g          890 t
ORIGIN      
        1 gattgtcgaa gtacagcccg cccaccccat cggaaccggc cagcatgccg atcactaaaa
       61 agagggtcag cgacggaatc cccaacttga cggacagctt gatcgtgaga atactgagaa
      121 tgaccaacgc ggaaaatatg atgatcaaca gctcaatcga tgtcggcatg gtctgtgctg
      181 gcatcacact ccgtcaagca ctgccttgaa tgatttcctg ataataggta tgaagcatcg
      241 cggcttgcgc cggctcgacg attaccaact gacgccggtc gaatgtgttg gcgcaataca
      301 aatagcgata cccttcggcg gtgcccagtt cgacatggag ttgacggccg tctgattctt
      361 cggcagtagc ttccttgagc actttcctat cgtttagttg ctcccacacc cgctgcgccg
      421 acggccaatg gttttcatag agcggcgcct ccggacttga agaacgggat gtcagcccga
      481 cacgatcaaa cggcagcccc tcatctggct ccgggtcctc attccaggca cacgcacttt
      541 ccttcccgag cgcgagcaca aagagcggcc cgtcgtcgga gacctcttca atcaacgaat
      601 agcggatcgg acgcggcggc tcgaacggct tcaagccgcg tgccgcaaac gcgcgctgca
      661 gtggaatccg tcgcgacagt tgacgtgtct tttcgaacac gacgagacgt ccactaggaa
      721 ccatagcggc ggccagtcga tcgagacgcc gaccgagccc tgttcgctcc tcaaacgccg
      781 cttgcgcttg cgcatccggc gcccgttcga atgtcctcca actgatgctc ggcagcccga
      841 gatcctgctc cgattgaacg agtgcatggg tggccacaac ccgttcgtat ttcccgatgg
      901 ctggtccttc caccagatcg gcacacacaa accgaaggtt cttcagaccc cattcgtcgg
      961 ccttgcgccg ggcctgagca atgcaggcag ctgaccgatc gaacccggta acttcggcct
     1021 gcggaaaccg ccgggcataa aacgttgtga ggattccggg accgcatccg aaatcaagaa
     1081 ccgtcttggc ctcgcccaga tgggcctcca cgactggcgc aatcgcgaca cagtactcgt
     1141 aacgctggct gtaaaggacc ggaagaatgc gtggcgcggc gctgagatcg tagaaggcca
     1201 tttcatcagc aggagagcca tgccgctttc gttcgacatg ttgatgcaag gctgaaagtt
     1261 cgtccgagga caactcctgg cgctgccagc gagcatacgc ctcgtcgctc gaaaaccgcc
     1321 gcaagcccca gtgggcaaga tgatcgtgaa gggattggtc tactgattgt gtcgtcatag
     1381 tgcttagaca cctcactcaa cacgcgctgc ggcgaggccg cggtgaccac aaggcgtacg
     1441 cgctgagggt acgttgagtg gttcgagcaa ctgagaacga agctgtagag cgttttcagt
     1501 gggcgtttac gggcgtttga gcatgatggc gtcaaagtac gtctctggta ccggatgagg
     1561 catgcgcagc tggccgtttc cgaatacgac gtatttctca caggtcaact gctctaaccc
     1621 aatcggaccc ttggcatgga gccgcgcact gctaagcccg atatcgctgc cgaatccgaa
     1681 tccttccgca tcgtgaagac gcgacgaggc gttcacaaag acggcactgg catccacttc
     1741 gcgcgtgaag cgcagagcgg acgaatagtt agaggtagcg atcacggccg tcatgcagag
     1801 cccgtgctct gcaatatgtg tcagcgcttc gtccatatcg gcaaccattt tcacggccag
     1861 cgtgggggac tggaactgtc gtgaccaatc ttcctccgcg gctggaatga tggacgtgtg
     1921 gccgctgatc ggcatttggc ccatcagtgc cagcgttttc ggacacccgc gcacgtcgat
     1981 tttgaactcg acgagtagac ggttgatcaa ggcgggcagg aattgccggg cgacgccctg
     2041 ctggaccagc aacgtatcca acgcattggt ggcggccggt cgctgcactt ttgaattgat
     2101 cacaacgttc tgtaccaacg gcagatccac gtcggcatcc acaaagaggt acgtctgacc
     2161 gccgtcgtgg cagaggaccg gaatccgggc tccttcgagc accgccttgc gcagcccggt
     2221 gcccccccgt ggaatgatgg catccagcgc cctgccggac ctgatcagtt ctaccgccac
     2281 gtctttctcg ggccggtcga tgaataccca ggagtctgga ggaatgccat gctgtccggc
     2341 tgcttcccgt aatcccttgc cgatgacctc gtgcgtttgg ctccactcgg gtgcgccgcg
     2401 aaaaacgctc acattgccgg acttgaggca caaggcgatc gactcgaccg tgaccagcgg
     2461 tggcaattcg gaaatgatgc cgatgacccc gatggaaacc cgtacacgcg acacttgaag
     2521 cccatcagga cgctcccatc gtcctgtcac ggctcccact ggatcgggct ggtcggcgat
     2581 ggctcgaagc cgctcggcca tctctttgac gtcgtccgcc gtcatacgga cccgggcaac
     2641 ggcagccttc agccggtcct tactgacttc actgccaaac gatttgccga gcgcgtccac
     2701 atctctctcg ttcgccgaga gaatggctgc ctcatcctcg gccacgcgct cggccatggc
     2761 tcgcagagct cggtccttga ctggcccggg tatcacggag gttttgcgca cagcatttct
     2821 acacaacttc aatagcttgt ctaagtaaag cttaattggc acttcaggca ttacgaccct
     2881 cgtgatgtgc gaagaatttt cctcagacgc ttgagtgccg gactataaca tgccgttttt
     2941 cggacagtaa acagccttat cccttgggcc tcgcgatgcg tatgtcttcc tcctgcttgc
     3001 cgagcgacca accaagccat gcccacaaac cggcgagcgg gaccagcacc aaggccatct
     3061 gtgtcacccc aacgccaagg gtttttacca tgctgaccat ccaaccggtc gccgcgtcgc
     3121 cgcctcgaga gaccgctgta tcgataaagt tttttgcttt gtacttctct tcgcgggtca
     3181 gcacggtgaa caacacctcc cgagtcggct tggcaatcgc atattcccca acgcggcgga
     3241 caaccgtaaa gaggatgaac acaagcaaca acggcatgac gccgaccaat gcgaagccgg
     3301 ctatgctgac agccggcaac acaaccagcg ctgaggccaa cccgaaccgt ctgatcatgg
     3361 ggccggtgat aaagaactgc gtggcaaatg tcagcacatt caccgcaagg tccatcactg
     3421 caaacagtct cgttcgaccc tggggcgaaa caacctgttc gccgatcagt ctcgtctgtt
     3481 ccagatagag aaacgtagcc gtcgtggtga ggcaaaacag atatccgcag atacctagga
     3541 gatagggcga ggaaagaact aagcgcattc ccgcccatgt acctccacca atggcgtcgc
     3601 ctcctcggtc gatatcctgt gcaggccgtt gtttcgacca gccatcgagc gctcggatac
     3661 agatccagca gatggccagg aatcccgtcg acaccagcat gagcacagga atcgggacgg
     3721 cataggtcag taccgtcgtg agcaaggggc ctaccacggc accggtgctc ccgcctgccg
     3781 caatgacgct gaacagccgg ctgccctggt ccggcgtaaa aatatcggcc atgaaactcc
     3841 agaagacgga aacaacaaac aggttgaaca ccgacagcca gacgaagaac aggcgcgcga
     3901 tccactgcgg cgcgacatga ctgttcatga tgagaaaaaa cccgatcaag tggctgccaa
     3961 aaaacgcata gacggccagc agcagtcgtt cgcgcggcaa gcgggctgac aaatagccga
     4021 agaatggtgt cgctgtcagc agcgtgacga aggtcgccgt catcatccag ggtaaatgct
     4081 ggacgccgcc ctgaatcgcc atttcatcgc gaaccgggcg cagcatgtaa tacccgcaga
     4141 gcaggcagaa aaaatacaga aacgaccaac agagtggcac gagttcttcc gactcggcac
     4201 cggtgagcga ggcgatggtc gttcgagagc ggcgggccac ggcggcatcc tgaccggccc
     4261 ttcgcgcgtt cgcaagcaaa atgcgccgga gagttttgga cttttttccc gtaccccgtt
     4321 atggtagtgg cgctatgcga atcatcgact tacgcagcga cacggtgacc aaaccgacgg
     4381 aggggatgcg cgaggcgatg gcgcgcgccg aggtcggcga cgacgtgttc ggcgacgacc
     4441 cgacggtcaa cgcgctgcag cagcgcatcg cggcgctgct cggcaaggac gcggcgctgt
     4501 tcgtgcccag cggcacgcag agcaacctgg tcgcgacgat gacgcactgc cgccgcggcg
     4561 acgaggcgat cgtcggcgag atggcgcacc tgtaccgctg ggaggccggc ggcgccggcg
     4621 tgctcggcag cgtcgtgcag ctgccgctgc cccatcaggc cgacggctcg ctggcgctcg
     4681 acgacatccg tgcggcgatc aagcccgacg acccgcactt cgcggtcaca cgcctgctgt
     4741 gcctcgagaa cacgctcggc ggcaaggcgg tgccgttggc ctggaccgcg caggccaccg
     4801 cgctggcgcg
//
LOCUS       DASTQY010000255         8544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_350038, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000255 DASTQY010000000
VERSION     DASTQY010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350038"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /gene="aroA"
                     /locus_tag="VFR82_08510"
     CDS             <1..1034
                     /gene="aroA"
                     /locus_tag="VFR82_08510"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU4501678.1"
                     /translation="LTGLLAGQDFFSVLTGDGSIRRRPMGRIVKPLREMGATIAGRKG
                     GELAPLAVTGTTLRAITYTSPVASAQIKSSLLFAALFAKGTSRFSEPRLSRDHTERLF
                     QFYGLPLTRDGLTLTMEGRPSVGWTAASELTVPGDFSAAAFFIVGGTIVPDSDVTITG
                     VGINPTRTGLLEILRRMGGDIQLQNRRDEAGEPVADIHVRSAPLHGVTIGPEWIPQTI
                     DEFPILCVAAAVASGQTVVTGAEELRVKESDRIATMSAELRAMGAQVHEKPDGLAIEG
                     LGHAQGNGRLRGTAGKSHGDHRVAMSIAVAGLTALDETRVHDADCIETSFPDFDRVLL
                     QLTSQKKGV"
     gene            1040..1699
                     /gene="cmk"
                     /locus_tag="VFR82_08515"
     CDS             1040..1699
                     /gene="cmk"
                     /locus_tag="VFR82_08515"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU4501679.1"
                     /translation="MIVAIDGPAGVGKSTVAKLLASRLGFVYLDTGALYRSVGLAVLH
                     HGIQPGDEKAVGGLLPKLSLKIEVTNNTVHVSLNGKDVTGELRTPAVSAAASIVSAIP
                     AVRAWLLPVQRQIGQKGSVVAEGRDVGTKVFPAAPVKFFLEADPTVRAERRHRELVAA
                     GHSGAIEQTSAELAGRDNRDRSRSAAPLVAADDAHHIDTSRLSAEDVVNRMLAVVTAK
                     L"
     gene            1696..2346
                     /locus_tag="VFR82_08520"
     CDS             1696..2346
                     /locus_tag="VFR82_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU4501680.1"
                     /translation="MSSVAYGILWVIVRAIAWLCFRFRVEGAVPKQGGVLVAANHASY
                     FDIPLLGCGMFRRAWYLGRSDLFIPGLKAILRWLGWIPLKLGRLDRKAIDQAIALIKA
                     GKVVVIFPEGGRSLDGRLRQAKAGLGMIVAQTGCSVVPAYLKGTFDVLPAGAKRPRFH
                     PVSVSFGAPLSFSATGNGDGKQFYQNVSRVVMERIAALGGVAPPQHHASAGARNAE"
     gene            2405..4117
                     /locus_tag="VFR82_08525"
     CDS             2405..4117
                     /locus_tag="VFR82_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU4501681.1"
                     /translation="MSTVSKTNEPQLDREALAALYEETFKNLEEGTITEGRVVALTKD
                     KVVVDIGYKSEGMIPSDQFSHDDLQHLKIGDRIQVYIEECEDADGNLILSKEKADKMK
                     IWEELEKLYKEEKSIDGKVVSRIKGGMMVDIGVKAFLPGSQIDLHPVRDLDGLVGRTF
                     PMKIIKINHRRGNVVVSRRVLLEETRDKKRQTTLATLKEGQLIQGMVKNITDYGAFID
                     LGGIDGLLHITDMSWGRVGHPSEMFNVGDRVEVSVLKYDRETGRISLGLKQKSADPWT
                     NVAGKYPIGTRVKGRVVSLTDYGAFVELEPGVEGLVHVSEMSWTHEVRHPSRVVAIGD
                     QVEAAVLNVDPASRKISLGMKQTAPNPWDMVEGKYPVGTRIEGKVKSLTDFGAFIGLD
                     EGIDGLIHISDMSWTKHIKHPSEIFKKSQKVEAVVLRIDKEKERLSLGYKQLSRDPWD
                     DEIPNRYRVGDPAVGKVSKVADFGIFVELDGGVEGLIHVSESGLDQSAKLEEKFKLQD
                     EVTAKIIKVDREERKIALSLRDHQLDSDRRHVDEYHATQGAPDQSLGRAAKQNRKRGA
                     DDEK"
     gene            4236..5072
                     /gene="sppA"
                     /locus_tag="VFR82_08530"
     CDS             4236..5072
                     /gene="sppA"
                     /locus_tag="VFR82_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEU4501682.1"
                     /translation="MAGIGIALLLLLNLLFPDLDLSTEDRVALIRVEGVILDAQSTVT
                     ELKRFGENPSIKAIVLRIDSPGGGVVPSQEIYDAVQRVRNKHNKAVIASMGTVAASGG
                     YYIAAATDRIIANPGTLTGSIGVIMETANVEGLLKKIGVEGIVVKSGKFKDVGSPLRK
                     MTEEEQKLLQSVMDDVHQQFIDAVAEGRALEPAAVQALADGRIFTGRQAKEAKLVDEL
                     GNLDDAIQLAADLAGIEGEPKVIEPRRRFSIREFLESRFSALFPKLDFYSGVSLKYLM
                     AF"
     gene            5093..5401
                     /locus_tag="VFR82_08535"
     CDS             5093..5401
                     /locus_tag="VFR82_08535"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU4501683.1"
                     /translation="MLVQAKGESDMTKAQIIERVSEQVTTLTKRQAEIVVNTIFDCVR
                     DSLKNGDKTEIRGFGSFRLRSRRMKEGRNPKTGATVAVPAKKVPFFKAGKELKELLNQ
                     "
     gene            5423..6424
                     /locus_tag="VFR82_08540"
     CDS             5423..6424
                     /locus_tag="VFR82_08540"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HEU4501684.1"
                     /translation="MAASKSNDTPAAEVRWTEGALKRMERAPVFLRGMVRRLAEKKAR
                     ELGYAEITEATLEQFKSQMMGGMGGASGMAQAVEDMAQGRLPWTAAAKERLNTVPEFM
                     RGMIRQIAEEVAKEGGHMEVNVDLFERVEAMGDVREAAAPPLAWTEGALARLQDKLKQ
                     SPPIAVEFVTDMLKRDTEDLAREKGLTQIDEQVLIQLWDTPQERVAWTDEAWKRLQTS
                     PDFVRSGIRKAAERRARKLGLKEIDSEHLTGFRNQAMMKAVKRIRSFGYNELTFDAFD
                     TALEKTKRLQGNEQAEKRLQEIRSHFADPDTKKPEGGTLGAELMGRFRRYLKGEGSL"
     gene            complement(6606..6926)
                     /locus_tag="VFR82_08545"
     CDS             complement(6606..6926)
                     /locus_tag="VFR82_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501685.1"
                     /translation="MGKTVKIDTTMYGIAEVLHWCHDRNKGRIPGVDTAGFEKMKALL
                     AEKPQSGDYFTLDQFWKKKVALDLTEEEVALIDRCLYDIPNLDNEPLPQIRHKFWPQQ
                     VAAH"
     gene            7139..8335
                     /locus_tag="VFR82_08550"
     CDS             7139..8335
                     /locus_tag="VFR82_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEU4501686.1"
                     /translation="MRGFTMRLSCTCLISGMLAVMTGACSKQDPYVPPDLFYYFASYK
                     VGKNPTTITPFDINQDGVTDLMTTNVSSNTLSLLIGNGDGTFHDQVQLHVCQEPRSLA
                     TGAFNADPYPDIALACAGGDQIAVLFGRADGKFQEGPQYPVHRTPVAIASDDVNGDGM
                     ADLVVALRNDKIKVFLGQGNGEFRHGAQYEYGDTPTSVALSDLNGDGKTDLVVTNGGP
                     MSNAVSIWLGNGDGSFQSPTDYRSGRRPLGVSFADFNNDRIRDLLVINGERDSFSTFL
                     GNGNATFQTAHDSGANAGPNFGLARDFNGDHRTDVAIVNLQSSDLSILFGRGDGTFEY
                     PPRNYRTKAGPFAIALYRVTSNEVEEPGLVTADNGDGSVSIFLHRGLKGGSTPKTAAS
                     SSQSTR"
BASE COUNT         1878 a         2216 c         2671 g         1779 t
ORIGIN      
        1 tgcttactgg gctcctggcc ggtcaggatt tttttagtgt cctcacggga gatgggtcca
       61 ttcggcgtcg tcccatggga cgaatcgtca aacctctacg cgagatgggc gccacgatcg
      121 ccgggcgcaa aggtggagaa ctggcgccgt tggccgtgac agggacaacg cttcgtgcca
      181 tcacctatac gtctcctgtc gccagtgccc agatcaaatc ctcactcctg ttcgcggcac
      241 tcttcgccaa aggcactagc cgtttctcgg agcccaggct ctcgcgtgat catactgagc
      301 ggctgttcca gttctatggg ttgccgctga cgagggacgg cctgactttg acaatggagg
      361 ggcgaccttc tgtcggctgg actgccgcat ctgaattgac cgtgccgggg gatttctcgg
      421 ccgcggcctt tttcattgtg ggcggtacca tcgtgcctga ttccgatgtc acgattaccg
      481 gcgttgggat caatccgacg aggaccggac tcctcgagat actccggcgc atgggcggcg
      541 acattcaatt gcaaaaccgc cgggacgagg ctggcgaacc ggtcgcggac atccatgtgc
      601 ggtccgctcc gctgcatggg gtgacgattg gtccagagtg gattcctcag accattgatg
      661 aatttccgat tctctgcgta gcggccgccg ttgcgtcggg tcaaaccgtc gtgaccggag
      721 ccgaggaatt acgggtaaaa gaaagcgacc ggattgcaac catgagtgct gagctccgcg
      781 cgatgggcgc acaggtacat gagaagcctg acggactggc gattgagggc ctggggcacg
      841 ctcaaggcaa tggacggctg aggggcacgg ccgggaagag tcatggggat catcgggttg
      901 cgatgtcgat tgccgttgct gggttgacgg cgctggacga aacgcgtgtg cacgacgcgg
      961 attgtattga aacgtcgttt cccgatttcg atcgtgtgct tctgcaacta acgtcgcaga
     1021 agaaaggcgt ttgagttcaa tgattgtggc aattgatggc ccagctggtg tcgggaaaag
     1081 cacggtagca aagttgctgg cctcgaggct gggatttgtg tatctcgaca cgggcgccct
     1141 gtaccggtcc gtaggcttgg cggttctgca tcatggtatc cagcctggcg acgagaaagc
     1201 agtgggcggg cttctgccga agctgtctct caagattgag gtcacgaaca acaccgtgca
     1261 cgtctcatta aatgggaaag atgtcaccgg agaattgcga acgccggcag tctctgcagc
     1321 cgcttcgatc gtctcggcca ttcccgcagt cagggcctgg cttttgccgg tccagcgaca
     1381 gatcggacaa aaaggctccg tcgtggcaga agggcgtgac gtcggcacga aagtgttccc
     1441 ggccgcaccg gtaaaatttt ttctcgaagc agatccgacc gttcgcgccg aacgccggca
     1501 tcgcgaactg gtggccgccg gtcacagcgg ggccatcgag cagacgagtg ccgagttggc
     1561 cgggcgggac aaccgagacc ggtcacgctc cgcggcgccg cttgtggcgg ctgacgatgc
     1621 ccatcatatt gatacgtccc gtttgtcggc cgaagacgtc gtcaaccgga tgttggcggt
     1681 ggtgacggcc aaattgtgag ctcggttgca tatgggatct tatgggtcat cgtacgggcg
     1741 atcgcgtggc tctgtttccg gtttcgcgtc gagggcgcgg tgccgaagca aggtggtgtg
     1801 ctcgttgccg ccaaccatgc cagctatttc gacattccgt tgctgggctg tggcatgttc
     1861 aggcgcgcgt ggtatctggg tcgaagcgat ttgtttatcc cgggcttgaa ggcgattttg
     1921 cgatggcttg ggtggattcc gctcaagctg gggaggctgg atcgcaaagc gatagatcag
     1981 gcgattgccc tgatcaaggc ggggaaggtt gtggtgattt ttccagaggg aggccggagt
     2041 ctcgatggtc gtctgcgaca ggccaaggct ggtttaggca tgatcgtcgc tcagaccggg
     2101 tgctctgtcg tgccggccta tctcaagggc acctttgacg tgttgcccgc cggtgccaaa
     2161 cggccacggt tccacccggt gtctgtgtct ttcggtgcac cgctgtcatt ctcggctacc
     2221 ggaaatggag acgggaagca gttttatcag aacgtgagtc gagtcgtgat ggagcggatt
     2281 gccgcactcg gcggggtggc tccgccgcag catcatgcaa gtgccggcgc tcgcaacgct
     2341 gagtgagcac cggtgttatt gatcatcagc acccgacagt cgtcggaaga gtaggatcgt
     2401 ccccatgagc acggtttcga aaacgaatga gccgcagttg gatcgtgaag cactagccgc
     2461 tctctacgag gaaacgttta aaaacctcga agaaggcacc atcacggaag gtcgcgttgt
     2521 cgccttaacc aaggacaagg ttgtggtcga tatcgggtac aagtctgaag ggatgatccc
     2581 tagcgaccag ttctcccatg acgacttgca gcacctaaag atcggcgatc ggattcaagt
     2641 ctatatcgag gaatgcgagg acgcggacgg caatctgatt ctttccaaag aaaaagccga
     2701 taagatgaag atttgggaag aattggaaaa gctgtataag gaagagaaga gcatcgacgg
     2761 taaagtggtt tctcgcatca agggcgggat gatggtggat atcggcgtga aggcgttcct
     2821 gcccgggtcg cagatcgatc tccatccggt gcgcgatctg gatggattgg tcggccgaac
     2881 gttcccaatg aagatcatca agatcaatca ccgccgtggg aacgtcgttg tgtctcgccg
     2941 cgtgctgctc gaagaaacca gagacaagaa acgccagacg actctcgcca ccttaaagga
     3001 agggcagttg attcagggca tggtcaagaa catcaccgac tatggagcct tcatcgacct
     3061 gggtgggatc gacggcttgc tccacatcac ggacatgtcc tggggtcgtg tcggacatcc
     3121 ttcggagatg ttcaacgtcg gcgatcgagt cgaagtgagt gtgctcaagt acgatcgtga
     3181 aaccggcaga atttcgctgg gcctcaagca aaaatccgcc gatccgtgga cgaacgtggc
     3241 cgggaagtat ccgatcggca cgcgcgtcaa gggtcgtgtc gtcagcctga ccgattacgg
     3301 ggcgttcgtt gagcttgaac cgggagtaga aggcctggtg catgtttcgg agatgtcgtg
     3361 gacccacgaa gtgcgccatc cgtcccgtgt ggtggccatc ggcgatcagg ttgaagccgc
     3421 cgtgctcaat gtggatcccg caagccgaaa aatttcgctc ggcatgaaac agacggcgcc
     3481 gaacccctgg gacatggtcg aaggaaagta tcccgtcggc acccgcatcg aaggaaaggt
     3541 gaagagcctg acggatttcg gcgccttcat cgggctcgac gaagggatcg acggattgat
     3601 ccacatttcc gacatgtctt ggaccaagca tatcaagcac ccgtcggaga tttttaagaa
     3661 aagccagaaa gtagaggccg tcgtcctgcg gattgacaag gaaaaggaac ggctgtcgct
     3721 cggctacaag cagctctcgc gtgatccctg ggacgacgaa attcccaatc gctatcgtgt
     3781 gggagatccg gccgtcggta aggtgagtaa agtggcggat ttcggaattt tcgtggaact
     3841 agacggaggc gtagaaggat tgattcatgt cagcgagtcg ggattggatc aaagcgcgaa
     3901 gctcgaagag aagttcaagt tgcaggatga agtgacggcg aagatcatca aggtggaccg
     3961 tgaagagcgg aagattgcgc tcagtctccg cgatcatcag ttggactccg accgtcggca
     4021 tgtggatgag taccacgcaa ctcaaggtgc tccggaccag agtctgggac gggcggccaa
     4081 acaaaaccgc aaacgcggag ctgacgacga aaaatagatt gtgaattctc ggcgccggag
     4141 cgtgctgctc ctgcgccggt tatccgtgga atcctatgga gcaggagacg acaggaaacc
     4201 cgcaaaagcg atcgtggctt cgccggatac ttctcatggc aggcattggt atcgctctgc
     4261 tgcttctcct caatttattg tttcctgatt tggatctttc cacggaggac cgtgtggctt
     4321 tgattcgtgt cgaaggggtc atcctggatg ctcaatccac ggttacggaa ttgaaacggt
     4381 ttggagaaaa tccatctatt aaagccattg tcttgcggat cgacagcccg ggcggcgggg
     4441 tcgttccgtc tcaggagatc tatgatgccg tgcagcgggt gcggaacaag cacaacaagg
     4501 cggtcatcgc ctcgatgggc accgttgcgg cttcgggtgg atattacatc gccgcggcaa
     4561 cggatcgtat catcgcgaat cccggcacct tgactgggag catcggggtg atcatggaaa
     4621 cggcgaatgt cgaagggttg ctcaagaaga ttggggtcga gggtattgtc gtgaagagtg
     4681 ggaaatttaa agacgtgggc tcgccgctca ggaagatgac cgaggaagag cagaaactgc
     4741 ttcaatcggt gatggacgac gtccaccagc aattcatcga tgcagtggcg gaaggacggg
     4801 cgctggaacc agcagcggtg caggcattgg ctgatggtcg aatctttact ggtcggcagg
     4861 ccaaggaagc aaagcttgtt gatgagttgg ggaatctaga tgacgcgatt cagttggcgg
     4921 ccgatttggc ggggattgaa ggtgagccca aggtcatcga gccacgccgg cgtttctcca
     4981 tccgggaatt tctcgagtcg cggttctcgg cgctgttccc gaagttggac ttctattcgg
     5041 gggttagcct caaatacctc atggcgtttt aatcggtcag agcggctggc cgctgctcgt
     5101 ccaagcgaaa ggggaatccg atatgaccaa ggcgcagatt attgagcggg tctctgagca
     5161 agtcacgacg ctgacaaaac gccaagcgga gatcgtcgtg aacacgattt tcgattgcgt
     5221 cagagactcg ctgaagaacg gcgataaaac ggaaatacgg gggtttggaa gctttcgcct
     5281 ccgatcacgg cggatgaagg aaggacgcaa tcccaaaacc ggtgccaccg tcgcagtgcc
     5341 tgcgaagaaa gttcccttct tcaaggcggg gaaagagctc aaggaattgc tcaatcagta
     5401 agggtaaata aaggaagatt taatggctgc atcaaaatcc aacgacacgc ctgccgcaga
     5461 agtccgatgg accgaaggcg cactgaagcg catggagcgc gctccggtat ttttgcgcgg
     5521 gatggtccgc cgtctggcag aaaaaaaggc acgggagctt ggctatgctg agattaccga
     5581 agccactctc gaacagttca agagccagat gatgggcggg atgggcggtg catcgggcat
     5641 ggcccaggcg gtggaagaca tggcccaggg ccggttgcca tggacggcgg cggcgaaaga
     5701 gcggttgaat accgtaccgg agttcatgcg cggcatgatc aggcaaatcg ccgaagaggt
     5761 cgccaaggaa ggtggccaca tggaagtcaa cgtcgacctc tttgaacggg tcgaggccat
     5821 gggggatgtg cgcgaggcgg ccgccccccc gcttgcctgg acggaaggag cccttgcgcg
     5881 tttgcaggac aagctgaagc agtcgcctcc cattgcagtg gaattcgtca ccgacatgct
     5941 caagcgtgat accgaagatc tcgctcgtga gaagggcttg acccagatag atgagcaggt
     6001 gctcatccaa ttatgggaca ctccacaaga acgagtcgcc tggaccgacg aagcctggaa
     6061 acggctccag acctctcctg acttcgtgcg aagcgggatt cgcaaggccg ccgaacgacg
     6121 cgcgaggaag ctcggtttga aggaaattga ctcggaacac ctgacggggt ttcgaaatca
     6181 agcgatgatg aaagccgtga aacggattcg ttcgttcggg tacaacgagt tgacattcga
     6241 tgcctttgat accgcgctag agaaaaccaa acggttacaa ggcaatgaac aggccgaaaa
     6301 gcgtctccag gaaattcgca gccactttgc cgatcctgac acgaagaagc ccgagggggg
     6361 aacgctgggt gctgaattga tgggccgctt ccgacgctat ctcaaggggg aaggaagttt
     6421 gtaacagtgg cctagagggg ttcctctctc gggaggttgg ccttcactct atcctgcgct
     6481 catccgtaat catgtcgcaa accgcctagt gctccctccc cagctctggc aacgcgaaac
     6541 cattcggctc taggccatgg ccagcgggcc aagccaagta agaaagtaag aatggcgggt
     6601 tggtctcagt gcgcggcaac ctgctgcggc caaaatttgt gtcgaatctg tgggagcggc
     6661 tcgttgtcga ggttgggaat gtcgtaaagg caacggtcga tcaatgccac ttcctcttcg
     6721 gtcaggtcca gggcgacctt tttcttccag aactgatcga gggtgaaata gtcgccggac
     6781 tgcggcttct ccgccaacag cgctttcatt ttctcaaacc cggcggtatc cacgccaggg
     6841 atgcggccct tattccggtc gtgacaccaa tgcaggactt ctgcaatccc atacatcgtc
     6901 gtatcgatct tcacggtctt acccatggat tcctccttgc gaaaggagtg tagtctaacc
     6961 cagattcgaa cggagtttca aaggccatct taccggcgca aatatgccgg ctcgaggcct
     7021 gttcgttgcc agaagaatgg cgccgccggt atactagcga gcctgcgaag cgcaggctac
     7081 gccggcatgc gggtcacgcc tgcgagccgt ggagggacgg gaggcggtcg tgtgggaaat
     7141 gcgtgggttc acaatgagat tgagctgcac gtgtctcatc tcgggcatgc tcgccgtgat
     7201 gaccggtgcc tgctcgaagc aggacccgta cgttccgccc gacctcttct attacttcgc
     7261 gagctataaa gtcggcaaga accccacgac gattaccccc ttcgacatca accaggacgg
     7321 cgtgaccgat ctcatgacga ccaatgtgag cagcaacacg ctgtcacttc tgatcggtaa
     7381 cggagacgga acgttccacg atcaagtgca gcttcacgta tgccaggaac ccaggtcgtt
     7441 agccaccggg gcattcaatg ccgaccctta tccagacatc gcgttggcat gcgccggtgg
     7501 ggatcagatc gctgtcttgt ttgggcgagc agacggcaaa tttcaagaag ggccgcaata
     7561 tcctgtccat cggacacccg tggcgattgc gagtgatgac gtgaacggcg acggcatggc
     7621 cgatcttgtg gtcgcactgc gcaacgacaa aatcaaggtg ttcttggggc aggggaacgg
     7681 tgagtttcga cacggggcgc agtatgagta cggcgataca cctacgtcgg tcgccttgtc
     7741 tgatctgaac ggcgacggga agaccgacct ggtcgtgaca aacggaggac cgatgtccaa
     7801 tgcggtttcc atttggcttg gaaatggaga cggcagcttt caatccccga cggattatcg
     7861 ctccggtcga cgccccttgg gcgtgagctt tgccgatttt aacaacgacc gcatccgtga
     7921 tcttcttgtg atcaatggtg agcgcgatag tttttccaca tttctgggaa atggcaatgc
     7981 cactttccag acagcacatg attccggcgc aaatgccggc ccaaactttg ggctggcccg
     8041 ggatttcaac ggggatcatc ggaccgatgt cgcgatcgtc aacctgcagt cgagcgatct
     8101 ctcaatcctt ttcggccgtg gagatggcac cttcgaatat ccaccgagga actatcggac
     8161 aaaagcaggt ccgttcgcta tcgccctcta ccgagtcacg agcaatgaag tcgaagagcc
     8221 tgggctcgtg accgccgaca atggcgacgg ctcagtctcg attttccttc atcgcggctt
     8281 gaaaggcggg tctacgccga agaccgccgc ctcctcatcc cagtcgactc gataggatat
     8341 tattgtggag tggcgaaaca gcgcagtcag cttgacagtc ctggacccct cggctacagt
     8401 ggccgacagg cgccgtttgg gtattagctg gctgcctcgt acggggacag cggtgagatc
     8461 cttttcgtgg tggttcggaa tgggcgcact cctgactctg tccgttctga tggttcaagg
     8521 aggcgtgcgc gacttgtttg acgg
//
LOCUS       DASTQY010000256         3452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_879505, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000256 DASTQY010000000
VERSION     DASTQY010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879505"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..2379)
                     /gene="bamA"
                     /locus_tag="VFR82_08555"
     CDS             complement(82..2379)
                     /gene="bamA"
                     /locus_tag="VFR82_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEU4501687.1"
                     /translation="MQRTRWLLVVFGLILLVAGGMDEAPAQTPIAKVASIDIRGNQKI
                     ELPAIQGRLTLKPGDNYSPENVRGQIKILYDTGYFEDVQVETESTPDGIALVFVVREK
                     PFITEIVFDGNEELSDDKLKEKITIKSQAFLDQQQAKESAEKIRLAYQEDGYFSAQVI
                     PIVQTLDVDRKRLTFFIKEGEKAKVKSVNFEGMRAASKSEIFKVTATREWVPWYGLVT
                     QLKLPSFLSDAGVLKREEMNNDVERIREVLLNKGFLNAQVGLPSVELSEDKKWFVVTY
                     AVVEGEPFTVAEIGFRGNTVFEDPELRDKMKIKPGEIFQRQKIRDEISRLTDLYGSKG
                     YAFAEVIPNVNPNAEERTATIILNIKEGEMMRIRQINVHGNDKTKDNVIRRELRVDEQ
                     DVIDTPSLKRSFQRLNNLNFFETVEILPQQVDADKVDLNVRVKEKPTGQFSIGGGFST
                     LDKLVAIADITEGNLGGNGWMGRIRGQLGQQRTLGLITFRNPYLNDSLTSLQLDIYRS
                     MTNYITYFEKKSGASITFGRWLSEYVTGSISFVAEELNFSDPTIDAPEIILSQLGSQT
                     TTGFRTTLARDTRDYFLDPRSGLRTAVGFDFGTPYLGGTNNFYKYYLDAIKYTPLPFD
                     TRFSIHARYGVADGIDGKPIPLTERFFVGGINTMRGFVFGRAGPVTPSGSLIGAAKEL
                     IFNNDFIFTISSEAKLNGVIFFDWGKGFDDNEPISFGALRKAAGLEGRWISPFGPLRA
                     AYGINLDPREGERKGVFEFTIGSLF"
     gene            2722..>3452
                     /gene="galU"
                     /locus_tag="VFR82_08560"
     CDS             2722..>3452
                     /gene="galU"
                     /locus_tag="VFR82_08560"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU4501688.1"
                     /translation="MSRADVRKAILPAAGLGTRFLPATKASPKEMLPLVDKPLIQYAV
                     EEAVASGIEDIIVITGRGKRAIEDHFDRSVELEENLKGNGKGQLLSQIRHISNLANFC
                     YVRQSEALGLGHAVLCAQHLIGDEPFAVMLGDEIIDASVPALAQLIHTYKKRHGAVLG
                     IQEVPHQEVNRYGIISPDRLSDGLYKVTDLVEKPAPSDAPSNLAVIGRYVLPPEIFPI
                     LRKTKPGKNGEIQLTDALRELAKSSP"
BASE COUNT          770 a         1027 c          870 g          785 t
ORIGIN      
        1 tgggatgacc cacagccttt gcgccaacac caacagtcct tgaactcgca gtcctagact
       61 cacccctcac ctcgacacgt attagaacaa cgacccgatc gtgaactcaa acacgccctt
      121 ccgctcgcct tcgcgaggat caaggttaat accgtaggca gcgcgcagcg ggccgaacgg
      181 cgagatccac cgtccctcca aacccgccgc tttgcgcaat gcgccgaacg agatcggctc
      241 gttgtcatca aatcctttcc cccagtcgaa aaaaatcacg ccgttcaatt ttgcctcgga
      301 tgagatcgtg aaaatgaaat cgttgttgaa aatgagctcc ttggcggcac cgatcagcga
      361 accgctcggc gtcacgggcc ccgcccggcc gaagacgaat ccgcgcatcg tattgatgcc
      421 gccgacaaag aaccgttctg tcaagggaat gggcttcccg tctattccgt ccgctacgcc
      481 atagcgggca tggatggaga atctcgtatc gaacggcagc ggcgtatatt taatcgcatc
      541 gaggtaatat ttatagaagt tgttcgtgcc ccctaaatag ggcgtcccga agtcgaaacc
      601 gacggcagtc cgcagaccgc ttcgcgggtc cagaaaataa tcccgcgtat cgcgggccag
      661 cgtcgtacgg aatccggtcg tggtttgact gcccaattgg ctcagaataa tctcaggcgc
      721 atcgatcgtc ggatcgctga aattcaactc ttccgcgacg aaactgatgc ttcccgtcac
      781 atattcggac agccaccggc cgaaggtaat gctcgcgccc gatttctttt cgaagtacgt
      841 gatgtagttg gtcatgctgc gataaatgtc caactgcaag gaggtcaggg agtcgttcaa
      901 gtacggattt cgaaacgtga tcaagcccaa agtccgctgc tggccgagtt gtccacgaat
      961 tcggcccatc catccgttgc ccccgagatt tccttcggtg atatcggcga tggccaccag
     1021 tttgtccagg gtgctgaacc cgcccccgat actgaactgc cctgtcggtt tttcttttac
     1081 ccgaacgttc aagtccacct tgtccgcgtc cacctgctgc ggcaggattt cgaccgtttc
     1141 gaagaagttg agattattga gccgttgaaa gctccgctta agcgacggcg tgtcgatgac
     1201 gtcctgttca tcgacgcgca gctcgcgtcg gatgacgttg tctttggtct tgtcattgcc
     1261 atgaacattg atctgccgga tgcgcatcat ctcgccttct ttgatattga gaatgatggt
     1321 cgcagtgcgc tcttcggcat tcggattcac attgggaatg acctccgcga aggcatagcc
     1381 cttgctgcca tagagatcgg tcagtcggct gatttcatcg cggatcttct gccgttgaaa
     1441 aatctctccc ggcttgatct tcattttgtc ccgtaactcc gggtcttcga agaccgtatt
     1501 gccgcgaaac ccaatctcgg ccacggtaaa cggctctcct tccactacgg cataggtgac
     1561 gacgaaccat ttcttatcct cactcaattc cactgagggt aagccgacct gggcgttcag
     1621 aaaccctttg ttgagcaaaa cctccctgat ccgctcgacg tcattgttca tttcctcgcg
     1681 tttcaggacg ccggcatccg agagaaaaga gggcagcttc agctgcgtca ccagcccata
     1741 ccagggaacc cattcgcgag tcgcggtaac cttgaatatc tcggacttgg acgcggcccg
     1801 catcccctca aagttcaccg atttgacctt ggccttttcg ccttccttga tgaaaaaggt
     1861 gagacgctta cgatcgacgt ccaaggtctg gacgatcgga atgacttgtg cgctgaaata
     1921 gccgtcctcc tgataggcaa ggcgaatttt ttcagcgctt tccttcgcct gctgctgatc
     1981 caaaaacgcc tggctcttga tcgtgatctt ttccttcaac ttgtcgtcgc tcaattcctc
     2041 attgccatcg aaaacgattt cggtaataaa gggtttctct cggaccacga acaccagagc
     2101 aataccgtcc ggagtcgact cggtctctac ctgcacgtcc tcaaagtatc cggtgtcata
     2161 gaggattttg atctggcccc gcacgttttc gggggagtag ttatcgccag gtttcaaggt
     2221 caatcgaccc tgaatggcgg gaagctcgat tttctggtta cctcgaatat cgatcgaggc
     2281 taccttggct atcggggtct gagccggcgc ctcatccatc ccacccgcca ccaggaggat
     2341 caggccgaac accaccagca accagcgagt gcgttgcacc aacgacaact ctctcactcg
     2401 caccagactc ccgaacgaac cacagagaat acagcttcca tctcaaggcc tcgatcagtg
     2461 gagacatgac gcccttcacc tgggcactgt cgccgcgcat ccgaagccgt cgatgacagc
     2521 agcaaggaag gttgctgacg tgagatgcct acgcgtgcgg cgttgcagca tcagccatga
     2581 acccgtgtga aaaactgtcg gattatatgg ggcaagtatt tcaatgtaaa gccgatctgg
     2641 tccgaaactc cgctatgacg taaccgtcga gccgattggc gattccttga catcacctac
     2701 cccatcacat actatcgccg tatgtctcga gccgatgttc gtaaagccat tctgcccgcc
     2761 gccggtctcg gcaccagatt tctgcccgcc acgaaagcct ctccgaaaga aatgcttccg
     2821 ttggtcgaca aaccgctcat tcaatatgcg gtagaagagg ccgtcgcctc cggcatcgag
     2881 gacatcatcg tgatcaccgg ccgaggcaag cgcgcgatcg aagatcattt tgaccgttcg
     2941 gtcgagctcg aagaaaatct caagggcaac ggcaaagggc agctgctcag tcaaatccgg
     3001 catatttcca acttggccaa cttctgttac gtgcgacagt ccgaggcgct gggactggga
     3061 catgccgtgc tctgcgctca gcatttgatc ggcgatgaac cctttgccgt catgttgggt
     3121 gacgaaatca tcgacgcatc agttcccgcg ctggctcaat tgatccacac atataagaag
     3181 cgtcacggcg ccgtcctggg cattcaggaa gtcccgcatc aggaagtgaa ccgctacggc
     3241 atcatttcac cggatcgact ctcggacggt ctttacaaag tgacggattt ggtcgaaaag
     3301 ccggccccat ccgatgcgcc ctcgaatctg gccgtcatcg gccgctacgt cttgcctccg
     3361 gagattttcc cgattctccg gaagaccaag cccgggaaga acggtgaaat ccaactgacc
     3421 gatgcgctga gagagttggc caagagttcg cc
//
LOCUS       DASTQY010000257         2802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_829963, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000257 DASTQY010000000
VERSION     DASTQY010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829963"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1788)
                     /locus_tag="VFR82_08565"
     CDS             complement(<1..1788)
                     /locus_tag="VFR82_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEU4501689.1"
                     /translation="MCVLKVGLWAFITLLGAGALAIVTGLLSPHEKVNGLWLVIAAAC
                     IYVLAYRFYGRWLACRVASLDNHRLTPAVRLNDGVNFHPTNRVVLFGHHFAAIAGAGP
                     LLGPVLAAQFGFLPGFLWLVIGAVLAGAVQDFIILVASMRRNGRSLPEIAHDELGTLT
                     GTATAVAVLFIVVVALAGLGFAVVNALYRNAWGTFTIAMTIPIGVFMGLYLQKFRPRA
                     VAEVSIVGVTLLIMTVLFGRVVAQSSFAPWFEYDKPTLVWLLAGYGFLASILPAWMLL
                     VPRGYLSTFMKLGVVLLLGLGVVLMAPTIEMPRVTIFSAGGGPIIPGSLFPFLFITIA
                     CGAVSGFHALVSSGTTPKMIEQESQAVVGYAAMLLESFVGVMALIAATVLVPGDYLAI
                     NTHLSTDVLTTMGFPPGRIAELSQLVEVDIAGRPGGAVSLAVGMASIFSALPGMAGLM
                     AYWYQFALVFEALFILTTIDTGTRVARYLIQEMAGRIYPPFRRFNWLPGVLASSGFVV
                     GAWAYLIGTGSISTIWPMFGAANQLLGTLALCIGTTVLIKMGKSSYLWITALPMVFIG
                     VVTLTGSYEMFWMFVRKAAASAAEDAVALY"
     gene            complement(1952..2386)
                     /locus_tag="VFR82_08570"
     CDS             complement(1952..2386)
                     /locus_tag="VFR82_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU4501690.1"
                     /translation="MSTYLPAVFSTVRTDGFDRQIDRMFDEALRTFGTAEGAWVPACN
                     AWEDSNGFYVQMALPGWEPKDVTLEVNNQMLSVKGEKTEDSSGSRKHLVREIADGRFV
                     RIFKLPTIVDHDKASANYKHGLLTISFPKREEAKCRRIVIEG"
BASE COUNT          704 a          817 c          742 g          539 t
ORIGIN      
        1 gtagagcgcg accgcatcct cggccgccga cgccgcggcc ttcctcacga acatccagaa
       61 catctcgtac gatcccgtca gcgtcacgac gccgataaac accatcggca gcgccgtgat
      121 ccacagatag gaggacttcc ccattttgat caacaccgtc gtcccgatgc aaagtgcaag
      181 cgttcccagt agttgattcg cagcaccgaa catcggccag atggtcgaaa tgctgcctgt
      241 cccaatgaga tatgcccagg ctccaacgac aaagccgcta ctggccagca cgcccggcag
      301 ccagttaaac cgacgaaacg gcggataaat acggccagcc atttcctgaa tgagataacg
      361 agccacgcga gtccccgtat caatggttgt cagaatgaat aacgcctcga ataccaacgc
      421 aaactggtac caataagcca taagtccagc catgccaggc aaggctgaga agatcgaggc
      481 cattccgaca gcaagagata ccgctccgcc tggacgtcct gcgatatcca cctcaaccaa
      541 ttgagacagc tctgcaattc gtcccggagg aaaccccatc gtggtgagga catcggtgga
      601 aaggtgtgtg ttgattgcga gatagtcgcc aggcactagc actgtggccg cgatcagcgc
      661 catgacgccg acgaaacttt ccaagagcat tgcggcatag cccaccactg cttgtgattc
      721 ctgctcaatc atcttgggcg tcgtccccga cgacacgagc gcatggaagc cagagacagc
      781 tccgcaggcg atggtgataa agagaaacgg gaagaggctg ccgggtatga tcggcccccc
      841 acctgcggaa aatatggtca ctcgtggcat ctcaatcgtt ggtgccatca gaacgacgcc
      901 caggcccagc aacagcacga cgccgagttt catgaaggtc gagagatacc cacggggcac
      961 caacaacatc catgcgggca gaatggaagc gagaaatcca tatccagcca gcagccatac
     1021 gagggtgggc ttgtcgtatt cgaaccacgg cgcaaaactc gactgtgcca cgactcggcc
     1081 gaataggacg gtcatgatta gtaacgtcac gcctacgatt gaaacttcgg cgaccgctcg
     1141 cggacggaac ttttgaagat acaaccccat gaatacgccg atgggaatcg tcatcgcaat
     1201 ggtaaatgtc ccccaggcat tgcgataaag tgcattgaca acggcgaaac ccaacccggc
     1261 caaggcgacg accacgatga acaagaccgc tacggccgtc gcagtccctg tgagagttcc
     1321 aagttcatca tgcgcgatct cgggtagcga gcggccattg cgtcgcatag atgccaccaa
     1381 aatgatgaag tcctgcacgg ctccagccag cactgcgccg atcaccagcc acagaaaccc
     1441 agggagaaat ccgaattgcg ccgctagcac tggcccgagc aagggaccgg ccccggcgat
     1501 ggcggcaaaa tggtggccga acagcaccac tctattcgtg ggatgaaagt tcactccgtc
     1561 attcaatcgc accgcgggag tgagccggtg attgtccaga gaagcgacgc gacaagcgag
     1621 ccagcggcca tagaagcggt aggccagcac atagatgcag gcggcggcta taaccaacca
     1681 gagcccattc accttttcat gcggagataa caggccggtc actatggcaa gcgcaccggc
     1741 accgagcaga gtgatgaatg cccacagtcc cactttcaac acgcacatgg gggcaatcct
     1801 agcacaatag cggatgatcc gacgctggcg atgtgtggcg atttgagaga gacaggctaa
     1861 tctgtggttg gatgagctgt aagtgtgccg aggaagtcgc cccgcacggc cggatgcatc
     1921 ggccggcgag gcgttcactc agtcaccggc attatccctc gatgacaatc cgtcggcact
     1981 tcgcttcttc gcgcttgggg aatgaaatcg tcaagagacc atgtttatag ttagccgacg
     2041 ctttatcatg atccacgatc gtcggcaact tgaagatccg cacgaagcgg ccatcggcaa
     2101 tttcacgcac gaggtgtttg cgcgaaccgc tgctgtcctc cgtcttctcg cccttgacgg
     2161 aaagcatctg attgttcact tccaaggtga cgtctttcgg ctcccaaccg ggaagggcca
     2221 tctgaacata aaatccgttt gagtcttccc aggcattaca tgctgggacc caggctcctt
     2281 cagccgtgcc aaacgtacga agagcttcgt caaacatccg atcgatttgc cgatcgaacc
     2341 catccgtccg aacggtcgaa aacacagcgg gcaaataggt gctcataact ctccctcctc
     2401 ctctctatga gaggtctggt tatgggttaa aggtttggag attggcactg ctggcgaagc
     2461 gcatacccgt tcaggcccct gttcagatct atgccgtagt accaatcagc tgcgtctaac
     2521 ggctttacag gtaattttgc gatgcgaaaa gtcaagaggg cagaactcca agcgatgttt
     2581 atagcggctg gatatccttt taaggaaaaa gaaacctccc gttaactgat cacctctgaa
     2641 gagggaggaa attctcggca gaaacggggg aaattgtggt ctgctttttc atcctgcgtc
     2701 tcttttgccc ttccatctcg gctcacagtg cgacatatcc gccaaggccc tgtatctgcg
     2761 gcgaaatcca atgcccttct ttgtaggtat ttctctggcc cc
//
LOCUS       DASTQY010000258         8170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_870537, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000258 DASTQY010000000
VERSION     DASTQY010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8170
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870537"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..284)
                     /locus_tag="VFR82_08575"
     CDS             complement(15..284)
                     /locus_tag="VFR82_08575"
                     /inference="COORDINATES: protein motif:HMM:NF039620.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEU4501691.1"
                     /translation="MVIAPIVIYGLCMLTALLCAWLLLQAYHKTRYRLLFWCSVFFCI
                     AAMNNVFLVVDKVVFPDVDLLVYRYGVALTGLMILLPALILEKEV"
     gene            complement(285..707)
                     /locus_tag="VFR82_08580"
     CDS             complement(285..707)
                     /locus_tag="VFR82_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501692.1"
                     /translation="MEGEVSRKHPMDEIVPAEVQRFVLDRIDSIAQLEAILMMRNAPD
                     TWWPSSTLAERLYIADKTCRAELDALCQRGLVLGKEQDIGWSYRYAPSNGELREFVDR
                     LVYYYAHHLVPISNLIHAKPRTRIHEFADAFALKKKDG"
     gene            complement(710..3133)
                     /locus_tag="VFR82_08585"
     CDS             complement(710..3133)
                     /locus_tag="VFR82_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4501693.1"
                     /translation="MPKANILIVEDEAVVAADIAGKLERAGYRSIAIASDGEDAIETA
                     KALAPDLVLMDIRLAGSLDGIKTAERIQAFRSIPIVYLTAHSDMATLRRAAATEPFGY
                     ILKPFDERDVTTQIEIALYKHQAERRLRESEERYRKLVETAMDSIITFDHAGVIHSCN
                     PATEQMFGYRVEEMIGRNISMLIPAWFAQGTEESSADAANRLMRLIGLWRELNGQRRQ
                     GDAFPVEIAVSESFSGGEIRYTGILRDVSERKRTEAELRWRAGLLAQTHDAVVVWRMG
                     GGVIYWNHGAEELYGWKVAEAAGQPIHQLLSTALPLSEEEFHRQLIEDGRWEGEIRQL
                     TKGGQSVVVESRMVTLTEDYGGILVLETNRDVTERRAMHDQVCRLAEGLEDRVKERTK
                     ELVQSQSLLRQLTSELTIAEQRERRRIATDLHDYLAQLLVCARLKVSQSRGRMAEPEV
                     EGWLGESDDILQQALTYTRSLVAQLTPMALHEFGLVAALKWLGDQMRQQYRLSVEVDV
                     QAGLSVKLPEDQAVLIFQSIRELLINVAKHARVDQATIRIQLKDGQLKIAVVDEGLGS
                     DTLTSSSGARSSKFGLFSIGERMRALGGTFDFTSSPGKGTIATLTMPLSPADAQSDVP
                     PVKTGETMTDRRRPSDLEYSQQSTAQRSAFRDTSELVSSASPLEERRIRVLLVDDHAL
                     VRSGLSSVLRYNTDLQVVGEAADGQEAVALAAALKPDVVIMDVNMPKMDGVQASRLIK
                     QDLPSTVVIGLSMHEGGHHEAAMRESGAAAYLTKDAAAERLIETILRCRAASLTGSSA
                     SFSSSALTE"
     gene            complement(3105..4250)
                     /locus_tag="VFR82_08590"
     CDS             complement(3105..4250)
                     /locus_tag="VFR82_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEU4501694.1"
                     /translation="MTTLKEPANILLVDDDSRNLDVLDSILACPDYRLFRARTADEAL
                     LALIAEEFAVIVLDVRMPEVSGYELAQLIKQRKRTQHIPILFLTAYYREDDDVLQGYG
                     AGAVDYLSKPVNPLILKSKVSVFVDLFRKTRALATMNRAMEVEIEERLKQLKASLHEK
                     DILLKEVHHRVKNNLQVISSLIDLQAERLPDPATRTLFRDTRDRVRSMALVHEKLYQS
                     ADLAHTELGAYIRNVMKELFLAHGEVSSKVTLQLQLEPVFLPVDMAIPCGLILNELAT
                     NSLKHAFVGREHGTIEIQLTSVRNESVRLRFRDDGWGLPAGLNLDAADSLGLRLIRML
                     SKQLRGEVKTHSGEGTTFELHFQLQNTRGGSPDEKAVLDAQGEHTHR"
     gene            complement(4370..>8170)
                     /locus_tag="VFR82_08595"
     CDS             complement(4370..>8170)
                     /locus_tag="VFR82_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4501695.1"
                     /translation="LGGQALVPGVAGTWKDLTDNVNSMASNLTAQVRNIAEVTIAVAN
                     GDLSRKITVDVRGEILQLKEAINTMVEQLRSFASEVTRVAREVGTEGRLGVQAVVPGV
                     AGTWKDLTDSVNAMGANLTAQVRNIAEVTTAVARGDLSRKITVDVKGEILELKNTINT
                     MVDQLNAFAAEVTRVAREVGTEGKLGGQAVVQGVAGTWKDLTDSVNVMATNLTDQVRG
                     IVKVVTSVANGNLRQRLTVEAKGEVAALGETINNMTDTLATFADQVTNVAREVGVEGR
                     LGGQANVPGAAGTWKDLTGNVNLLAANLTTQVRAIAEVATAVTKGDLTRSIQVETRGE
                     VAELKDNINTMIDNLRVTTERNQEQDWLKTNVAKFTRMLQGQRDLFTVGQMLLSELAP
                     LVEAQEGTIYQMDIVEGQRPSLRLLASYASHREPPERVGVGEGLIGQCAVEKQRILLT
                     DVPPSYTVIHSSLGEALPANIVVLPVLFEGQTKAVIELASLHSFRVSHITFLEQLTQS
                     IGVVLNTIEATMRTENLLQQSQQLTSELQSQQKELTQTNEELERKAKQLAERNTEVER
                     KNKEVEQARRALEEKAAELALTSKYKSEFLANMSHELRTPLNSILILGQQLGDNTTGN
                     LTQKQVEFAKNIHSAGSDLLNLINDILDLSKIESGTVTVEAEEMTFSALRESVERNFK
                     HIAEQKNLPFHLEFESGLPRAFTTDPKRLQQILKNLLSNAFKFTSHGHVSMNVRIATE
                     GWTMEHPVLSRSHTVMALAIADTGIGIAPEKQKLIFEAFQQADAGTSRKYGGTGLGLA
                     ISRELATLLGGEIRLTSEPKQGSTFTLFLPLVYTGPSSAGVRPSAGTVAIPGQAPSLA
                     ILPVVGEEKIADDRDTVQNGDLVVLIVEDDPHYARVLLGLARDKGFKGIVATRGKQAL
                     DLALEYVPAAITLDIFLPDMLGWTVLNNLKLNPKTRHIPVQIISLEEERQHGLAHGAF
                     SYMVKPVTTEGLESCFDRIRNFITPHLKRLLVVEDNELESRSITELLLHDDIEIINVG
                     TGAAALDALRTQSFDCCVLDLRLPDMSGFELLDQVKEDPTLGNVPIVVFTGKDLSTEE
                     ERRLKAVAKSVVLKDVQSPERLFDETALFLHRVVADLPEPKQQLLERLHQSNEALHGH
                     KVLVVDDDARNIFALTTVLENQEMDVMSATNGRHAIELIENTPDLSVVLMDIMMPEMD
                     GYTTIREIRKKPEFRSLPILALTAKAMKGDREKCLEAGASDYVAKPVNTEQLLSMLRV
                     WLYR"
BASE COUNT         1589 a         2490 c         2297 g         1794 t
ORIGIN      
        1 atgtgccagc ggtgtcatac ttccttttcc agaatcagtg ccggcaacag aatcatcaga
       61 ccggtcaggg caacaccgta gcggtacacc aacaagtcga cgtctggaaa cacgactttg
      121 tccacgacaa gaaagacatt gttcatcgcg gcaatgcaga aaaatacact gcaccagaac
      181 aataagcgat accgcgtctt gtggtatgcc tgcagtagga gccacgcgca gagcaacgca
      241 gtgagcatgc agaggccata gatgacgatc ggggcgataa ccatctatcc gtccttcttt
      301 ttcagcgcga aggcatcggc gaactcatgg attcgcgttc gaggttttgc atgaatgagg
      361 tttgagatgg gcacgagatg atgagcgtag taatacacga gccggtcgac gaattcacgc
      421 agctctccat tcgatggggc gtagcggtac gaccagccga tgtcctgttc ctttccaagc
      481 accagtcctc gctgacagag cgcatccagt tcagcccggc aggtcttatc ggcgatgtac
      541 aaacgctcgg ccaacgtgct cgacggccac caggtatctg gagcgttgcg catcatgagg
      601 atcgcttcga gttgggcgat ggagtcaatg cggtccagca cgaatcgctg aacctcagca
      661 ggaacgatct catccatggg atgcttgcgg gatacttcgc cttccacgtt tactccgtga
      721 gagcggacga cgaaaaggac gcactgctcc ccgttaaact tgccgcacgg catcgtaaaa
      781 tggtttcgat caatcgctca gccgcggcat ccttcgtcaa ataggcggcc gcccccgact
      841 ctctcatggc ggcctcgtgg tgtccgccct catgcatgga caggccaatg accacggtcg
      901 aagggaggtc ttgcttgatg agtcgtgagg cctgcacccc gtccatcttg ggcatgttga
      961 cgtccatgat cacgacgtca ggcttcaacg ctgcggctaa cgcaacggcc tcctggccgt
     1021 cggccgcctc cccgaccacc tgcaggtccg tgttgtaccg tagcacgctc gagaggccgc
     1081 ttctcacgag cgcatgatca tccaccagca gcacccggat gcggcgttct tcgaggggtg
     1141 atgccgacga aaccaattcc gaagtgtccc gaaacgcaga tcgttgggcc gtcgactgtt
     1201 gagaatactc caggtcgctc ggccgccggc ggtccgtcat ggtctcacct gttttaaccg
     1261 gtggaacgtc ggattgtgcg tcggccggcg atagaggcat ggtgagcgtc gcgatcgtgc
     1321 cttttccggg tgaggaagtg aagtcaaatg tccctcccag cgcacgcatc cgttcgccga
     1381 tgctgaataa accgaatttt gaagaccgtg cgccggatga ggacgtgagc gtatcggaac
     1441 ccaggccctc gtcgacgacc gcaatcttga gctgtccatc cttcagctgt atgcggatcg
     1501 tcgcctgatc aacgcgagca tgttttgcca cgttaatgag cagctctcga atggattgaa
     1561 agatgagaac tgcctggtct tcaggcagct tgacggacag gcctgcctga acatcaacct
     1621 ccaccgacag gcggtactgc tggcgcatct gatcgcccag ccatttcagg gcagccacca
     1681 ggccaaactc atgcaacgcc atcggcgtca gctgggcaac cagcgagcga gtatacgtca
     1741 gggcctgttg gaggatgtcg tccgattccc cgagccatcc ttccacctcc ggctcagcca
     1801 tccggcctcg agactgggat actttgaggc gcgcacatac cagcaattgc gcgagataat
     1861 cgtgcaaatc ggtggcaatc ctccgtcgtt cccgctgttc ggcaatcgtc aactcggatg
     1921 tcagctgacg cagcaaactc tgggactgga ccaattcctt ggtccgctcc ttgacgcgat
     1981 cctcgagacc ttccgccaag cggcacactt gatcatgcat cgccctccgc tcggtgacgt
     2041 cacgattcgt ctccagcacg agaatgccgc cgtaatcctc ggtgagagtc accatgcgac
     2101 tttccacgac gacggactgc ccgcctttgg tcagctgcct gatctctccc tcccagcggc
     2161 catcttcgat caattgccgg tggaactcct cctcggaaag cggcaaagcc gttgagagaa
     2221 gctggtggat cggctggcca gccgcttcag cgaccttcca cccgtacagt tcctccgcgc
     2281 cgtgattcca ataaatgaca ccgccgccca tccgccacac gacaaccgca tcgtgcgtct
     2341 gcgccagaag gccggcgcgc caccgcaatt ccgcttccgt tcgcttgcgt tcggagacat
     2401 cacgcaaaat acctgtatag cggatttcgc cgcccgaaaa actttcgctg acggcaatct
     2461 ccacagggaa ggcatcgccc tggcggcgtt gcccgtttaa ttcgcgccag aggccgatca
     2521 gccgcatcaa ccggttcgcc gcgtcggcgg aagattcctc agtcccctgc gcgaaccagg
     2581 cggggatgag catcgagata ttgcgcccga tcatttcttc gacccggtac ccgaacatct
     2641 gttcggtagc cggattgcac gagtggatga cgccggcgtg atcgaacgtg atgatgctgt
     2701 ccatggcggt ttcgaccagc ttgcgatacc gctcctcgct ttcccgcaag cgccgctccg
     2761 cctgatgctt gtagagcgcg atttcaatct gagtggtgac atcgcgttcg tcgaagggct
     2821 tgagaatgta cccgaatggc tccgttgctg ctgcgcgccg aagagtggcc atgtctgaat
     2881 gggcggtgag atagacaatc ggaatactgc ggaacgcctg gatgcgttcg gcggtcttga
     2941 tcccgtccag cgagccggcc aaccggatat ccatgaggac gaggtccggc gccagcgcct
     3001 tcgccgtttc gatggcatct tcaccgtcag atgcgatcgc gatggagcgg tagccggcgc
     3061 gttccagttt gcccgctata tcggccgcga cgaccgcttc atcttcaacg atgagtatgt
     3121 tcgccttggg catcgagcac cgccttttcg tctggggaac cgccacgtgt gttttgaagc
     3181 tggaagtgca attcaaacgt cgtgccttca cccgaatgcg tcttcacttc cccccgtagc
     3241 tgcttgctca acatgcggat caggcgcaag cccaacgaat cggccgcgtc caggttaaga
     3301 ccggcaggaa ggccccaccc gtcgtcccgg aaacgcagac gcaccgattc attccgcaca
     3361 ctggtcaatt gaatttcgat cgtgccgtgt tctcgtccta cgaacgcatg cttgagactg
     3421 ttggtggcca actcgttcag aatcaatccg cacggaatgg ccatgtcgac tggaaggaag
     3481 actggttcga gctgcagctg caatgtgacc tttgacgaca cttctccatg cgccaggaac
     3541 agttccttca tgacgttccg aatgtaggcg ccgagttccg tatgggcaag atcggcggat
     3601 tggtacagtt tctcgtgtac cagcgccatc gaccggaccc gatcgcgcgt gtcgcgaaag
     3661 agcgtccgcg tggcagggtc cggcaggcgt tcagcctgca aatcaatgag actggaaatg
     3721 acctggagat tattcttgac gcgatggtga acctccttga gcaaaatatc cttctcatgg
     3781 agcgaggctt tgagctgctt gagccgctcc tcgatctcga cttccatcgc ccgattcatc
     3841 gtagcgagag cccgggtctt acgaaagagg tccacgaaga cagagacctt cgatttcagg
     3901 atgagtggat tgacgggctt gctcagatag tccactgctc ctgccccata accttggagc
     3961 acgtcatcat cttcgcggta gtaggcggtc aggaatagga ttggaatatg ctgtgtccgt
     4021 tttcgctgct tgatgagttg ggccagttca tacccgctga cctccggcat ccggacgtcg
     4081 aggacgatca cggcaaactc ttcggcaatc agcgccagca aggcttcgtc agcagtgcgg
     4141 gcgcggaaca agcgataatc gggacaggct agaatgctgt ccagaacgtc gaggtttctg
     4201 gaatcgtcgt ccaccagcaa gatattcgcg ggctctttca aagtggtcat ggcgtcagta
     4261 atctgttcct ccgttccatg taagaacatc ccctatgact ctgacccgct gaggctgagt
     4321 gtacgtgcca agtttttaca atttcaaccc ggccctcgtg ctacctcctc taccggtaca
     4381 accaaacccg tagcatggac agcagttgct cggtattgac cggtttcgcg acgtaatcgg
     4441 aggcaccggc ctccaagcac ttctcacggt cgcccttcat ggccttggcg gtcaaggcga
     4501 ggatgggcag tgaccgaaac tccggctttt tccggatttc tcgtatcgtc gtatacccgt
     4561 ccatctcggg catcatgatg tccatcaaca ccacgctgag atcaggcgtg ttctcaatga
     4621 gctcgatggc atgacgaccg ttcgtggcgc tcatcacgtc catttcctgg ttttccagaa
     4681 cggtggtcag ggcgaaaatg ttgcgcgcgt cgtcgtccac cacgagaact ttatggccat
     4741 gaagggcttc attggattgg tgcagtcgct ccagcaattg ttgcttcggc tccggcagat
     4801 ccgccacgac ccggtgcagg aacaacgccg tctcgtcgaa caggcgctcg ggtgattgaa
     4861 cgtccttcaa caccacgctc ttcgccacgg ccttgagacg gcgttcttct tccgtgctca
     4921 aatcttttcc ggtgaacacg acgatgggca cgttaccgag cgtcggatcc tccttgacct
     4981 gatcaagcag ttcaaagccg ctcatgtccg gcaggcgcag atcaagtacg caacagtcga
     5041 acgattgggt gcggagcgca tcgagcgctg ccgctccggt gcccacattg atgatctcga
     5101 tgtcgtcgtg caggagcagc tccgtgatgc tgcggctttc cagctcgttg tcctcaacca
     5161 caagcaaccg cttgagatgt ggcgtgatga agttccggat ccggtcgaag caggactcga
     5221 gtccctcggt cgtgaccggt ttgaccatgt aggaaaaggc gccgtgggcc agtccgtgct
     5281 gacgctcttc ctccaatgaa atgatctgaa cgggaatgtg tcgtgtcttg ggattcagct
     5341 tgaggttgtt caagaccgtc caaccgagca tgtcgggcag aaagatgtcc aaagtaatcg
     5401 cagcagggac gtactcgagt gcaagatcca gcgcctgctt gcctctcgtc gccacgatcc
     5461 ctttgaagcc tttgtcccgg gccagcccta agaggacccg ggcgtaatgt ggatcgtctt
     5521 cgacaatgag gaccacaaga tcgccgttct gtaccgtatc cctgtcgtct gcgatctttt
     5581 cttccccaac cacgggcaag atcgccagcg acggcgcttg acccgggata gcgacagtgc
     5641 cggccgatgg acggacacct gcgctcgagg gacccgtata gaccagcggc agaaacaagg
     5701 tgaacgtgct gccttgcttg ggttcgcttg tcagccggat ctcgccgccc aacagggtcg
     5761 ccaactcgcg gctgatcgcc agacccaggc cggttccccc atatttgcgg ctcgttcccg
     5821 catccgcctg ttggaacgcc tcgaagatca atttctgttt ctcgggcgca atgccgatgc
     5881 ccgtgtcggc aatcgcgagg gccatgaccg tatgcgaccg gctcaacacc ggatgctcca
     5941 tcgtccatcc ttccgttgca atccggacgt tcatcgacac atgcccgtga gacgtgaact
     6001 tgaaggcgtt cgacaggagg tttttgagga tctgctgcag gcgtttgggg tcggtcgtaa
     6061 atgcgcgagg caatccgctc tcgaactcca gatggaacgg cagattcttt tgttcggcga
     6121 tgtgcttgaa gttgcgctcg acgctttccc ggagcgcgct gaacgtcatc tcttcggcct
     6181 cgaccgtaac cgtcccggac tcgatcttcg acaggtccaa gatatcgttg atcagattga
     6241 gcaggtctga accggcgctg tggatgtttt tcgcgaattc gacctgcttc tgcgtcaaat
     6301 tgcccgtcgt attgtcgcca agctgctgcc cgagaatcag aatgctgttc agcggggtcc
     6361 gcaactcgtg cgacatgttc gccaaaaatt ccgatttgta tttcgaggtg agcgcgagct
     6421 ccgccgcctt ttcctccagc gcgcggcgtg cctgctcgac ctctttgttc ttccgttcga
     6481 cttccgtatt gcgctcggcc agctgcttgg ccttgcgctc gagctcctcg ttcgtttgag
     6541 tcaactcttt ctgctgcgat tgcagttccg acgtgagctg ctgcgactgt tgcagcaggt
     6601 tttccgttcg catcgtggct tcaatggtgt tgagcacgac gccgatactt tgcgtgagct
     6661 gctcgaggaa ggtgatgtgg gaaaccctga acgaatgcaa cgacgccaat tcgatgaccg
     6721 ccttcgtctg tccttcgaac aacacgggca gcaccacaat attggccggc aacgcttcgc
     6781 cgaggctaga gtggatcacg gtgtaggagg gcggcacgtc ggtcaagaga atccgctgct
     6841 tttccacggc gcattggccg atcaatccct cgcccactcc cacacgctcc ggtggttcgc
     6901 gatgactcgc gtaggacgcc agcagacgca aagagggcct ctgcccttcc acgatgtcca
     6961 tctgatagat ggtgccttcc tgcgcttcga ccagcggggc cagttccgaa agcagcattt
     7021 ggccaaccgt aaaaagatcc cgctgcccct gcaacatacg ggtgaacttt gccacgttcg
     7081 tcttcagcca gtcctgctcc tggttgcgct ccgtcgtcac gcgcaggttg tcaatcatcg
     7141 tattgatgtt gtccttgagc tctgcgacct ctccgcgcgt ttccacctga atggaccgcg
     7201 tcaggtcgcc ttttgtcacc gcagtcgcca cctccgcgat ggcgcgcacc tgcgtggtga
     7261 gattggccgc caataggttc acgttgccgg tcaagtcttt ccacgtcccg gcggcaccgg
     7321 gcacgttggc ctggccgccc agccgtcctt cgacgccgac ctcacgggcg acgttcgtga
     7381 cttgatcggc gaaggttgcg agcgtgtcgg tcatgttgtt gatggtttct cccaaggcag
     7441 ccacttcgcc cttggcttcc acggtcaacc gctgtctcaa attgccgttc gccaccgacg
     7501 tcacgacctt cacgatgccg cgcacctggt cggtcaagtt tgtcgccatg acgttgacgg
     7561 agtctgtcaa atccttccag gttccagcga cgccctggac gacggcctgg ccgcccagct
     7621 tgccctccgt accgacttcg cgcgcgacac gcgtcacctc ggcggcgaac gcgttcaact
     7681 ggtccaccat cgtattaatg gtgttcttta actcgagaat ctcgcctttg acatcgaccg
     7741 tgatcttgcg cgagaggtcg ccgcgggcga cggccgtcgt gacctcggcg atgttgcgca
     7801 cctgcgccgt caggttggcg cccatggcgt tcaccgagtc ggtgaggtct ttccaggtac
     7861 cggcgacgcc tggcaccacg gcttgaacgc ccaaccggcc ttctgttccc acttcgcgcg
     7921 cgacacgagt gacctccgag gcaaaggacc gcaattgctc gaccatcgtg ttgatggcct
     7981 cctttaactg gaggatttcg ccacgcacgt cgaccgtgat cttgcgagac aagtcgccgt
     8041 tggccacggc aatggtgacc tcggcgatgt tccggacctg ggcggtcagg tttgatgcca
     8101 tcgagttcac gttgtcggtc aggtccttcc aggtgccggc gacaccgggc acgagtgcct
     8161 ggccgccgag
//
LOCUS       DASTQY010000259         7576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1586049, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000259 DASTQY010000000
VERSION     DASTQY010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7576
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586049"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFR82_08600"
     CDS             complement(<1..317)
                     /locus_tag="VFR82_08600"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4501696.1"
                     /translation="MNRSHMPSQSCAGFVAPLMLAVALVGACATSDEMKQKSKGYYQE
                     GMASLEHDRQKAYVSFQKAVQLDPQNKEARYGLGHILVYQGKLSQAEREFREAIKIDE
                     NYS"
     gene            complement(314..1228)
                     /locus_tag="VFR82_08605"
     CDS             complement(314..1228)
                     /locus_tag="VFR82_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU4501697.1"
                     /translation="MGKLLVVGSVALDTVKTPFGEATEVLGGSATYFSTSASYFTAVD
                     LIAVVGEDFPSQHLAFLKSRNIDLAGLERRSGATFRWRGEYTHQLNEAHTLDTKLNVF
                     ETFRPKIPESYRSPDVLFLGNIDPELQLDVLNKLPRPPLVACDTMNFWISGKRDALWR
                     VLEKVDILIINDGEARALGGDSNLVKVAASVLARGPKHLIIKRGEYGVLMFNENQVFG
                     APAFPLEDVRDPTGAGDTFAGGFLGYLAATGNRSVESFKQAIIFGSVMASFTVEAFSL
                     DRLRVLDYKEIQDRFRAFKRLTHFEDLP"
     gene            complement(1232..2131)
                     /gene="mtnP"
                     /locus_tag="VFR82_08610"
     CDS             complement(1232..2131)
                     /gene="mtnP"
                     /locus_tag="VFR82_08610"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEU4501698.1"
                     /translation="MAKFTRASRADIGIIGGSGLYDMEGLHQVKRVDVRTPFGSPSST
                     IVLGELDGVRVAFLSRHGIGHRYNPSEINYRANIYALKAIGVRRVMSVSAVGSMKESI
                     KPGDVVLPHQFIDLTKRRLSTFFDGGLVAHVAFGEPVCASLSASLLGAGRSVGATVHQ
                     GGTYVCMEGPQFSTKAESHLYRQWGVSVIGMTNLPEAKLAREAELCYATVALVTDFDC
                     WHETEECVSVEAILATLCQNVALAKRLLRAALKSAASAATCECQRALQHAIVTAPDKI
                     PPALRRRLALLIERVLPPVKGKR"
     gene            complement(2156..3175)
                     /gene="miaA"
                     /locus_tag="VFR82_08615"
     CDS             complement(2156..3175)
                     /gene="miaA"
                     /locus_tag="VFR82_08615"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU4501699.1"
                     /translation="MTTCPHLIMPSLSERQLRRKPLVVILGPTAVGKSRIAVEVAKYL
                     GSEILTADSRQVYRGMDVGTDKPTLIARQGIGHHLIDLVDPDEPFNAGLFRRHAAAAI
                     EECYRRRMLPLLVGGTGLYVRTLLQGLCEAPPSDPLVRARLRHDACEQGREQLFARLV
                     LVDPLTASKLHPRDTSKILRALEVYLQSGLPLSSFQSKHGFSERPFSALVVGLNRGRD
                     HLYRRIEERIDWQLTHGLIDETQTLLNRGYRRDSPAMKGLGYRQVASYLAGDCNWDEM
                     VSEFKRDTRRFAKRQMTWFRREPELTWLMIEESEPAEQTVSRVIDMIHPFLEGLDGDI
                     ETHQS"
     gene            complement(3172..4953)
                     /gene="mutL"
                     /locus_tag="VFR82_08620"
     CDS             complement(3172..4953)
                     /gene="mutL"
                     /locus_tag="VFR82_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU4501700.1"
                     /translation="MPAARCSPTIRVLPADVISRIAAGEVIERPAAVVKELIENSLDA
                     GSRHITVDIKDGGTSLIRVADDGQGITRSDLPLAFERHATSKLKSDAELSSIGTMGFR
                     GEALPSIASVSKVLVATATNEEHIGAQLSLIGGVPQQLVDAPAVIGTRIEVSDLFYNQ
                     PARRKFLKSIPTEFSHISTVVQQAALAWPFVHFRLTHNGQEVLNHAAVASGRDRILQV
                     YRQPFLERTVEVQGRIAGCTISGVMIDPVQARSSRAPQDLFVNRRPVRNAAVFHAVSE
                     GYGSLLAKGHHPTYVLFLDLDPERIDVNVHPAKREVRFADQEGIHRFVRQSVRHAFSG
                     VERKAVLRLADDPATATVMAPVGDGGSSIESSGWRHPEQTDQRLASMPGSLFAAERTV
                     AYSASQDVTITPLGQMLHTYLIAQVGRELHVIDQHTAHERVLFQRLWRTWMTRDMVTQ
                     PLLIPEPIDLTVAQYTLMQKYTGDLEQLGLVLEPFGANTVVLRAVPVGVGSVDGASVV
                     RDVLEDLAEWASISSLDARVQAVLATLACKSAVQAGRIMALPEIEQLVRDWVDEGVMS
                     TCPHGRRTAFRLSADELDTLFGRTGWS"
     gene            complement(4981..5748)
                     /gene="ybgF"
                     /locus_tag="VFR82_08625"
     CDS             complement(4981..5748)
                     /gene="ybgF"
                     /locus_tag="VFR82_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEU4501701.1"
                     /translation="MHDGMMMVAVGFVTVTALTVSGCAKHADFVELRDHLATVAKSQD
                     QDQKRLDALQRRMESLERVKDVEAPKTKLEDFNARLQKLEARVAKLDEGATASPTLKS
                     EAASADPPKTIKPQRAGSEGAPIIPNVPAITPTSAFNLSYNDYLNGKYDLAVSGFQRF
                     VKDFPGTSLTPNAHYWLGESYYQQKDYARAMQAFEYVSTEFPGNEKVPAALYKLGLAA
                     AETGDLVKSKKNLKRVLEEFPTSDEAKLAKNKLADIR"
     gene            complement(5751..7313)
                     /gene="ybgF"
                     /locus_tag="VFR82_08630"
     CDS             complement(5751..7313)
                     /gene="ybgF"
                     /locus_tag="VFR82_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEU4501702.1"
                     /translation="MNIGWNVASVGTVVCVFSAALLHGCVAQQADLKSAERRFKESSD
                     ALAQRGAQQRQELEELKSQELPKLRGDLERAHHQAQEIQRAQDDLKQRSAVLEQQTRK
                     LEQLSGKLDADSANRFAQVRESLNAQDLKNKADRDQLRVDVNTRLDDVNHQMEVLRKD
                     IIDAVQKTNTAFAKSLDVKLEEQRKALADNQARTEQLSAKFAQFNQALTGFRESLSSL
                     GDRIAQEEQAGKALATKVEADTKASTAHINETTRSMSGHLGEVNKSVNAVAQKLAARI
                     DEQMGEINKNVTKIDGRVAEQDRRIDGLTKSLDHVTQEVHTRGTTPKAGQRQQPAGTK
                     ATQRSSSVGPAEGVSHHEAEPVAAARQDPETVNHVAERTVAADEQAESVALAASARPV
                     ERSDKAEYERVLAFFKEGNLEGSREGFSAFLTEYPNSELAPNARYWLGESHYGKRDYK
                     QAIDSYDRVELDYPQSEKVPAAILKKGFAYLALKDKKRASSAFKQVVTLYPKSPEAGK
                     AYDKLNQLKELR"
     gene            complement(7339..>7576)
                     /locus_tag="VFR82_08635"
     CDS             complement(7339..>7576)
                     /locus_tag="VFR82_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4501703.1"
                     /translation="VAMVKVEGHCDERGTSAYNLVLGEKRAKAVRNFLVELGISANRL
                     SVVSYGKERPSCAEHAESCYQQNRRGHLVVKSGK"
BASE COUNT         1531 a         2289 c         2091 g         1665 t
ORIGIN      
        1 tccgaataat tctcgtcaat cttaatcgct tcccgaaatt ccctttcagc ctgggacaac
       61 ttgccttgat acaccagaat atgaccaagc ccgtaccgcg cttccttgtt ctgggggtcg
      121 agctgcaccg ccttttgaaa cgagacgtag gctttttgac ggtcatgttc cagactcgcc
      181 atcccttcct gataatagcc cttcgacttt tgcttcatct catcggaggt cgcgcaggct
      241 ccgacgagtg caaccgccag catgagtggc gcaacaaatc cggcacacga ctgggacggc
      301 atgtgcgacc ggttcacggt aggtcctcaa aatgggtcag acgcttgaaa gcccggaagc
      361 gatcctggat ctctttgtaa tccaggactc gcaaacggtc aagactaaag gcttctaccg
      421 taaacgaggc catcacgctt ccaaaaatga tggcctgctt gaacgactca acggaccggt
      481 ttcctgtagc ggccagatag cccaggaagc cgcccgcaaa ggtgtcccct gcgccagtcg
      541 gatcacggac gtcttccagc ggaaacgcgg gagccccgaa cacctggttc tcgttgaaca
      601 tcagaacccc gtactcgcct cgcttgatga tcagatgttt gggccctcgt gccaggacgc
      661 tagcggccac cttgacgagg ttggagtccc cccccagggc ccgtgcttca ccatcgttga
      721 tgatgagaat gtctaccttc tcgagcaccc gccacagagc gtcccgcttg ccgctgatcc
      781 agaaattcat cgtatcgcag gccactaagg gcggccgtgg caacttgttc agtacatcca
      841 gctgcaactc agggtcgatg ttgcccagaa acaacacgtc gggagatcga taggattccg
      901 ggatttttgg acggaaggtt tcaaacacgt tcaacttcgt atcgagcgtg tgagcctcgt
      961 tgagctggtg tgtgtattcc cccctccacc taaatgtcgc gccggaacgc cgttcgagtc
     1021 cggcaagatc aatgtttcgg cttttgagga acgcgaggtg ctgagaggga aagtcctcgc
     1081 ctaccacggc aatgaggtca accgcagtga aatagctcgc ggacgtggaa aaatatgtgg
     1141 ccgatcctcc gagcacctcc gtggcctcgc cgaacggtgt ttttacggtg tccagtgcga
     1201 ccgatccgac gaccaaaagt ttgcccatga ttcaccgttt tcctttcaca ggtggaagga
     1261 cccgttcgat caagagcgcc aatctccggc ggagggcggg gggaatcttg tccggagcag
     1321 tgacgatcgc atgctgcaag gcacgctgac attcgcaggt cgctgctgag gcagccgatt
     1381 tcagggccgc acgcagcagc cgtttggcca gcgcaacatt ttgacacaag gtggcaagaa
     1441 tggcctccac gctgacgcac tcctcggtct catgccaaca atcgaagtca gtcaccagag
     1501 ccaccgtggc ataacatagc tcagcttcgc gggcgagctt ggcttcgggc agattcgtca
     1561 tgccgatcac gctcacgccc cactgacgat ataaatgtga ttcggctttt gtcgaaaatt
     1621 gcgggccttc catgcagacg taggtgccgc cctggtggac cgtggctccg accgaccgac
     1681 cggctccgag cagcgaagcg ctgagtgagg cgcacaccgg ctcgccgaag gcaacatgcg
     1741 caacgaggcc cccgtcaaaa aatgtcgaga gtcgtcgctt cgtcaaatcg ataaattgat
     1801 gcggtaggac cacgtcgccc ggcttaatag attccttcat gctgcctacc gcgctcacgg
     1861 acataactcg ccggacaccg atcgccttca aggcatagat gtttgcccga taattgattt
     1921 cggagggatt atatcggtgg ccgataccat gccgcgacaa gaatgcaacc cgaactccgt
     1981 caagctcgcc gagcacgatg gtactcgaag gcgatccgaa tggtgttcgc acgtcgacgc
     2041 gtttgacttg atgcagccct tccatgtcgt ataagccgct tccgccgatg atgccgatgt
     2101 ccgcacgaga cgcccttgtg aacttggcca cgatgccgcc tttccctacc aagaattagg
     2161 actggtgtgt ttcaatatct ccgtccagtc cttcgagaaa aggatggatc atgtcgatca
     2221 cccggctcac cgtctgctcg gctggctcgg attcttctat catgagccag gtcagctccg
     2281 gttcccggcg gaaccaggtc atctgccgct tggcaaaccg cctggtgtcg cgcttgaact
     2341 cgctcaccat ttcatcccag ttgcagtcac cggcaagata gctggccacc tgacggtatc
     2401 ccagcccttt catcgccggg gagtctcgcc tgtaccccct attaagcaat gtctgtgttt
     2461 cgtctatcaa cccgtgggtg agttgccaat caatgcgctc ctcgatacga cgataaaggt
     2521 ggtctcgccc ccggttcaat ccaacgacta gagcagaaaa cggtcgctcg ctaaatccat
     2581 gcttcgattg gaaggacgat agaggcaagc cggattggag atacacttcc agggcccgca
     2641 ggatctttga ggtgtcccgg gggtgcagct tggacgctgt caggggatcg accagcacca
     2701 ggcgggcaaa cagctgctca cgcccctgtt cacacgcgtc gtggcggagc cgcgcccgca
     2761 caagggggtc gctcggcggc gcttcacaaa gcccttgcaa gagcgtccgc acgtagaggc
     2821 cggtgccgcc gaccagcaag ggaagcatcc gccgtcggta acactcttcg attgcggcag
     2881 cagcatggcg ccggaaaaga ccggcattga agggttcgtc gggatccaca agatcgatga
     2941 gatgatggcc aattccttgg cgggcaatga gcgtgggctt gtcagtgcct acatccattc
     3001 cgcgatagac ctggcgcgaa tccgccgtca gaatttcgct tcccagatat tttgccactt
     3061 cgacggcaat ccggctcttg ccgacggcgg tgggtccgag aatcaccacg aggggcttcc
     3121 ggcgcagttg tcgctcggag agtgagggca tgatgagatg agggcaggtc gtcacgacca
     3181 tccggtccgg ccgaagagcg tgtccagttc gtccgccgag aggcggaagg ccgtgcgcct
     3241 tccatgcggg cacgtggaca tcaccccttc gtctacccag tcgcgcacca gctgttcgat
     3301 ttcgggaagt gccatgatcc gtccggcttg gacggcactc ttgcaggcca gcgtcgcgag
     3361 aaccgcctgc acacgtgcgt ccagcgagga aatactcgcc cattcagcca agtcctcgag
     3421 gacatcgcgt acgactgacg cgccgtcgac ggaccctacg ccgacaggca ccgccctcag
     3481 gacaacggta ttggcaccga acggctcaag aaccagaccc agctgctcaa gatcgcctgt
     3541 atatttctgc atcagcgtat attgggcgac ggtcaaatca atcggctctg gtatcagcag
     3601 cggctgggtc accatgtcac gcgtcatcca tgtgcgccac aagcgttgaa acaacactcg
     3661 ttcatgagcc gtgtgctggt caatcacgtg caactcgcgc ccgacctgcg cgatcaaata
     3721 ggtgtggagc atctgtccca aaggcgtaat cgtaacatcc tgcgacgctg aataggcgac
     3781 cgtgcgttcc gccgcaaaca gtgagcccgg catagacgcc aggcgctggt ccgtttgctc
     3841 cgggtgccgc catccggaag attcaatgga agagccgccg tccccaacgg gcgccatcac
     3901 cgtggcagtc gcagggtcgt cggccaagcg aaggaccgct ttgcgctcga ctccactgaa
     3961 ggcatgtcgc acgctctggc gtacgaaccg gtgtatgcct tcctgatctg caaaccggac
     4021 ttcgcgcttt gcaggatgca cgttcacgtc gatgcgctcg ggatcgagat cgagaaacag
     4081 cacataggtc ggatggtggc cttttgcgag caaggagcca tacccttcac tgaccgcatg
     4141 gaacacggcc gcgttgcgga ccggacgtcg attcacgaaa agatcttgcg gcgccctcga
     4201 ggagcgcgcc tggacaggat cgatcatgac gccgctgatc gtgcatccgg caattcggcc
     4261 ctgcacctca actgtcctct cgagaaacgg ctgccgatac acctgcagaa tccgatctcg
     4321 accggaagca acggccgcat gattcagtac ttcctgcccg ttatgggtca gtcgaaaatg
     4381 tacgaacggc caggcgagtg ccgcctgttg aaccacggtg ctgatatgtg agaattccgt
     4441 tggaattgac ttgagaaatt ttcttcgagc cggctggtta taaaagaggt ccgagacttc
     4501 aatcctggtt ccgatcaccg ctggagcatc gaccagctgt tgaggcaccc cgccgatcaa
     4561 tgacagctgc gccccgatgt gttcctcgtt tgtggccgta gccaccagca ctttggaaac
     4621 ggaggcgatg ctgggcagtg cttccccccg gaatcccatc gtgccgatgg aggagagctc
     4681 cgcatcggac ttcagcttgc tcgtggcgtg acgctcgaag gcgaggggaa ggtcggaccg
     4741 tgtgatgccc tggccatcgt cggccacacg gatgagtgaa gtacccccat ccttgatgtc
     4801 gacggtgatg tgacggctgc cggcatcgag actgttttcg atgagttctt tgacgacggc
     4861 ggcaggccgc tcgatcacct ctccggctgc aatccggctg atgacatcgg ccggaagcac
     4921 tcggatcgtc ggagaacagc gtgcggcggg caccagctta ggctcccaac acccatccca
     4981 tcatcgaatg tctgccagct tgttcttggc aagcttggct tcgtccgagg tgggaaattc
     5041 ctcaaggacc cgcttcagat ttttcttgga cttcacgaga tcgccggtct ccgctgctgc
     5101 cagccctaac ttgtagaggg cagccggtac cttttcattg cccggaaatt cggttgagac
     5161 atattcgaac gcctgcatcg cgcgcgcata atccttctgc tggtaatagg actctccaag
     5221 ccaatagtgg gcattcggtg taagcgaggt ccccgggaag tccttgacga aacgctgaaa
     5281 gcccgaaacg gccagatcgt atttgccgtt caggtaatcg ttatacgaca ggttgaacgc
     5341 tgaagtggga gtaatagcag gaacgttggg gataatgggt gccccttctg atccagcccg
     5401 ttgaggcttg atcgttttgg gggggtcggc cgaagcggct tcagatttaa gagtcggaga
     5461 agcggtcgct ccctcgtcca atttggctac acgggcttcc agtttctgca gccgagcgtt
     5521 gaaatcctcg agtttggttt tcggcgcttc cacatccttg actcgttcca gcgactccat
     5581 gcggcgctgc agcgcgtcta accgcttttg atcctggtct tgggacttgg ctacagtcgc
     5641 cagatggtcg cgcaactcca caaaatccgc gtgcttggca catcccgaga ccgtcaaggc
     5701 cgtcacggtt acgaatccca ccgccaccat catcatgccg tcgtgcaccg ttatcgtaat
     5761 tcctttagtt ggttgagttt gtcataggct ttgccggctt ccggactctt gggatacaga
     5821 gtcacgacct gcttgaaagc cgaggaagcc cgcttcttgt ctttcagcgc aagataagcg
     5881 aagccttttt tgagaatagc cgccggcact ttctcgctct gaggatagtc cagttcgact
     5941 cggtcgtagg aatcgatcgc ctgcttataa tctcgtttac cataatgaga ttcacctaac
     6001 caatagcggg cattgggtgc caactctgaa ttcggatatt cggtcaagaa tgccgagaac
     6061 ccctcgcgag atccttctaa gttaccttcc ttaaaaaagg ccaacacgcg ttcgtattca
     6121 gccttatctg accgttcgac cggacgtgca gaagcagcga gggcaacgga ttccgcctgt
     6181 tcatctgccg caaccgtccg ctctgcgaca tgattcaccg tctccggatc ctgccgtgcc
     6241 gcggcaaccg gttcggcttc atgatgagat acgccttccg ccgggccaac cgaggaggac
     6301 cgctgcgtgg cctttgttcc cgctggctgc tgacgctggc cggctttggg agtcgtgccg
     6361 cgcgtatgaa cttcctgcgt cacatgatcg agcgactttg tgagtccatc gatccgacgg
     6421 tcctgctcgg ccacccgccc gtcgatcttc gtgacgttct tgttaatctc gcccatctgt
     6481 tcatcaatac gggcagcgag tttctgagcg accgcattga cactcttatt gacctcaccg
     6541 agatgtcccg acatcgacct cgtggtctca ttgatgtgag cagtggatgc cttcgtgtcg
     6601 gcttcgacct tggtcgccaa ggccttgccg gcctgttctt cctgagcgat ccgatctcct
     6661 aacgaggaca aggactctcg aaatcccgtt aaggcctgat tgaactgcgc aaatttcgcc
     6721 gacagttgct cggtacgggc ctgattgtcc gccaacgcct tgcgctgttc ctccaatttc
     6781 acatctaagc tcttggcgaa cgccgtatta gtcttttgga ccgcatcgat gatgtctttg
     6841 cgcaacactt ccatctggtg attgacgtcg tcgagtcgcg tattgacgtc cacccggagc
     6901 tgatcgcggt ccgccttgtt cttcaggtct tgcgcattga ggctttcgcg cacctgcgca
     6961 aaacgattgg cgctgtcagc atccagcttt ccagacaact gttcgagctt cctggtctgc
     7021 tgttcgagga cggcggatcg ctgcttcagg tcatcctgtg cccgttgtat ttcctgagcc
     7081 tgatggtgtg cccgttcgag gtcgccccga agtttcggca gctcctggct cttcagctcc
     7141 tcgagttctt gccgctgttg cgcgccccgc tgcgccaagg cgtcacttga ttccttgaac
     7201 cggcgttcgg cgcttttcaa atcggcttgc tgggccacgc agccatgcaa gagcgcggcg
     7261 gaaaacacac agaccacagt cccgacactt gcgacattcc acccgatgtt catgtgttac
     7321 ctacgtccgc ctcgttggtt acttgcctga cttaacaacc agatgtcccc gccggttctg
     7381 ctgatagcag gattccgcat gctctgcgca ggacgggcgc tcttttccat aggatacgac
     7441 tgacaaccgg ttcgcgctga tgcccaactc caccagaaaa tttcgcacgg ccttcgcgcg
     7501 cttctcgccc aacaccagat tgtacgccga cgtcccccgc tcgtcgcaat ggccttccac
     7561 tttgaccatg gccact
//
LOCUS       DASTQY010000260         5754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_30844, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000260 DASTQY010000000
VERSION     DASTQY010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30844"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..973)
                     /locus_tag="VFR82_08640"
     CDS             complement(242..973)
                     /locus_tag="VFR82_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU4501704.1"
                     /translation="MKSPASMGLNRTGIDTSPEQFQEMLTGLEEFPPTTTGDEETLEV
                     SRAAYIRDADPIGTVPPPGTVKGVVKDGMQKLMGRNAEVFIDALGGRLAFERTGTRLY
                     DAFLEKCEVRSEEAAILPIDRLRQFRQEEAAHLEMIWDALRQLGADPTCVTPLADANG
                     VASIGVMQLLADPRTSIAQSLHAILIAELVDNDGWQQLIKLALTLGQNAMAEQFRRAM
                     AEEDQHLTVIRQLVEQLTAAQAGAD"
     gene            complement(970..2148)
                     /locus_tag="VFR82_08645"
     CDS             complement(970..2148)
                     /locus_tag="VFR82_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501705.1"
                     /translation="MGLNLLRDKGTPIDQQKFTWKDMVQKPVSKLDIDAFTRVRIILM
                     NGIESEAVRFSHACARMNKTMQAPLATVRRKEQHQQTLINWLLPADQSPLETTIGYEQ
                     VAIEVTAAMARVEPDPYIAQVLRFGLLEDFDHLYRYSALLDRLQGIDANTITQSYTDI
                     LPGRPTVDEHRDPLDDLREHYDKEKAQPLTKLHALTIVAGEHQTHDYYMHYGPWFADP
                     LARQLYAEIASIEEQHVTQYESIIDPTESWIEKWLLHEANEVYNYYSCVSQESDPRIK
                     AIWERFLDYELGHLQYAITVCKDMKRRDPEEFLPAKLPEPIAYQSNREYVRQVLRDEL
                     DLRAAGVQFVNKSEEPERSLAYRHQMNADGSPSETVADGWIWTPGGELASNIRMKEAA
                     "
     gene            complement(2373..2879)
                     /locus_tag="VFR82_08650"
     CDS             complement(2373..2879)
                     /locus_tag="VFR82_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501706.1"
                     /translation="MPNLDVTSILLWSGATAGIAVIYYSFLLAREWFRQQHAAGKGVF
                     RKIAMEPETSLNAHIASQWTDGRLRLDSILTLIKSLANLLEKEVKTLRGLDRSDPQYE
                     GEVRQMVAPTLRCFTFDKHLRDECLVLIPLLRFCAQDLAQSQGLDMTQQLTALDEIQA
                     AIETANLH"
     gene            3488..4342
                     /locus_tag="VFR82_08655"
     CDS             3488..4342
                     /locus_tag="VFR82_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4501707.1"
                     /translation="MKHLSQDLFRVGLSAILTSAGLLAVPCSLMAQGSLMAQGPPTPE
                     EQRAREAESAADTQRAERQREAARADKRNERDEPGEFYVAGFGGYTIGHGFNDVEGTG
                     ALSGTNLGDIDQNDSGVYGAKVGYFMPRRLNWLGVEMEAFNTTPHLEETALTTGAHLR
                     VTTLAFNVIARAKMMCERHDDHDRTGRPAGDRHDFCRLQPYAGVGLGVFFARAKDATG
                     SASDNGVPGLNALAGVRYYFTERVALFAEYKYNRATFNFDDIGGTGAGVKGDYSASHI
                     VGGLSLHF"
     gene            4758..5249
                     /locus_tag="VFR82_08660"
     CDS             4758..5249
                     /locus_tag="VFR82_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501708.1"
                     /translation="MYPFATCVIALGMFMGLSDNQAYGQTDEASIFLVQGGTGAGSGS
                     QGMGTGKSGTGKETDAGRGAGKKSPFGADQPTSGDFSSNPSNATTSDSKPEDLDKTIA
                     GSEQPAGIGEKGSGQTRSTTERSGGPQPSASESISKSGKEHGEGKTERAMKELQSQKP
                     KNE"
     gene            5465..>5754
                     /locus_tag="VFR82_08665"
     CDS             5465..>5754
                     /locus_tag="VFR82_08665"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEU4501709.1"
                     /translation="MVERKLPRTMSDANIVSVINTIDQGELDTAYLALVKASVPVRQY
                     AAHLVHEHTARLDKNRTLVHSMNLEPDTARLSSVLRDINQAQIAVLRTKSGL"
BASE COUNT         1314 a         1521 c         1540 g         1379 t
ORIGIN      
        1 ccaaatagaa cccatcctat aatcgacatg gcggcacctc ctcgttatgg gcggcgctgg
       61 ttaccctaaa ctgatgactt ccggtcagcg ccacttttct tgctggctcc cacctgctca
      121 gcccagctta taaggtcgtt cttcttctcg gcgtatccct atacacaagg tctcaggctg
      181 agggtgaaaa taactatctc tgtcgatgat atttcacgtt ctttctgaaa attgatcgtt
      241 atcaatctgc gccagcctga gctgcagtca gttgttctac aagttgtctt attacggtca
      301 agtgttggtc ttcttccgcc atagcccgcc gaaattgttc ggccatcgca ttttgaccca
      361 gcgtgagagc cagctttata agctgttgcc agccatcatt gtccaccaat tcggcaataa
      421 ggattgcgtg cagactttga gcaatggacg tccgtggatc ggctagcaac tgcatcaccc
      481 cgatggaggc tacaccgttt gcgtcggcca ggggagtcac acatgttggg tccgctccca
      541 actgacgcaa ggcgtcccat atcatttcca ggtgggcggc ctcctcttgt cgaaactgcc
      601 gtaaccggtc gatggggagt atggcggctt cctccgatcg gacctcacac ttctcgagga
      661 aggcatcgta tagccttgta cccgtgcgct caaaggccag gcgtccccca agtgcatcga
      721 taaacacttc cgcattgcgt cccatcaatt tttgcatgcc atctttgacc acacctttta
      781 cggttcccgg cggtgggacc gttccgatcg ggtcagcgtc gcgaatgtac gcggcgcgag
      841 aaacctcgag cgtttcctcg tctccggtgg tcgtcggagg gaattcctca agacctgtca
      901 gcatctcttg aaattgttcg ggcgaggtat caataccggt gcgattcaaa cccatcgaag
      961 cgggggattt catgctgcct ccttcatgcg gatgttcgaa gccaattcac ctccaggtgt
     1021 ccagatccat ccatcggcga ccgtctctga cggcgaaccg tctgcattca tttggtgccg
     1081 gtacgccaac gaacgctcag gctcttcact cttattcaca aattgaacgc cagcggctcg
     1141 gagatcgagc tcatcacgaa gaacttgccg gacatattcc cgattgcttt ggtatgcgat
     1201 gggctccggg agcttcgccg gtaagaattc ttccggatcc cgacgcttca tgtctttaca
     1261 aacagtgatg gcatattgga gatgtcccag ttcatagtcg agaaagcgtt cccaaatggc
     1321 tttgatgcgc ggatcacttt cttggctcac gcaggagtag tagttataaa cttcgttcgc
     1381 ttcatgtagc agccacttct caatccagct ttccgtcgga tcgatgatgg actcgtattg
     1441 cgtcacatgc tgctcctcga tcgaggcaat ttctgcgtac agctgacggg ccaatgggtc
     1501 cgcaaaccac ggaccgtagt gcatgtagta gtcatgtgtc tgatgttcgc ctgccacgat
     1561 cgtgagtgca tgaagtttgg ttaaaggctg cgccttttcc ttgtcatagt gctcgcgcaa
     1621 gtcgtccaaa ggatcgcgat gttcatccac agtcggtcga ccgggcaaga tgtctgtata
     1681 actttgcgtg atggtgttgg catcgatccc ttgtaatcgg tccaggaggg ctgaataccg
     1741 gtagagatgg tcgaagtctt caagtaaccc aaagcgcagg acttgcgcaa tgtagggatc
     1801 cggttcaaca cgggccattg ctgcagttac ttcgatagcc acttgctcgt agccgattgt
     1861 cgtctcaaga ggagattggt cggcgggcaa aagccaattg atcagtgtct gctgatgctg
     1921 ctccttccgt ctaacggtgg ctaagggagc ctgcatggtt ttattcatcc gcgcacaagc
     1981 atgactaaac cgcacggctt cagattcaat accgttcatc aagatgattc gcacccgggt
     2041 gaaggcatcg atatcgagtt tgctgaccgg cttttgaacc atgtccttcc aggtgaactt
     2101 ttgctgatcg ataggcgtgc ctttgtcgcg caataaattt aatcccattt gaagtcccct
     2161 ttagtggtcg gatgtggtgg tgcgataccg ccgtgtggga gtatttctct acactaacag
     2221 gcgaacacgt aattccaaca ctagcagtcc ccctcagaaa accatttgag gtattccagt
     2281 ttgctgagat gcctagggcg gctctcagtg tatagattcc ccctcacttt actgatcaca
     2341 acagcacaag agacggaatg acggaatgag tgtcaatgca ggttggccgt ctcgatggca
     2401 gcttggatct cgtcaagggc ggtcaattgt tgtgtcatgt ccaggccctg cgattgtgcg
     2461 aggtcctgag cgcagaatcg caggagcgga atcagcacaa ggcactcatc tcgcaaatgc
     2521 ttatcgaagg tgaagcagcg cagggtgggt gctaccattt gacggacttc cccttcatac
     2581 tgcggatctg accgatcgag tccacgcagt gtcttcactt ccttctctaa caagttcgca
     2641 agtgatttga ttagagttag gatcgagtcc aaccgaagac gaccgtcagt ccattggctg
     2701 gcaatgtgtg cgttgagaga cgtttcgggc tccattgcaa ttttgcggaa gacgcctttg
     2761 ccggcagcat gctgttgtcg aaaccattcg cgcgcgagta agaacgaata atagataacg
     2821 gcgatgcctg ccgtagcgcc cgaccacaat aaaatggagg taacgtccag attcggcatt
     2881 acagcttgtc cctcgtcgta aatccggaag cgcgtttcgg gagtcttcgc tatacactcg
     2941 gccctctcgg tgcctccaat tgcaccggcc cctatcacga gaacaatttg tcccggaaag
     3001 tgccgcggtc ccgagacttc gcattcgatg ctaaacccct ccccaagggt aaagctgacc
     3061 aagactgctc aaacgccgaa atacgtgcca tctgttttca taggaccgga cggatccatg
     3121 catgaccggg aagcggtcgt tgcgaccgac cgctttcacc aatgggtgca gtttcaactc
     3181 tcttcatcgc tgttcgcttc agtttcgttc tcatcgacga ggatcctgtg atcacggaat
     3241 gaggcagacg gaagcgaagc caagtttcct cgcgctgatt ctcgcttact caggatagaa
     3301 cgtcttcaaa tggacattta gttcagtccg gcgattcagt gagactggta actcactgag
     3361 gcctgtctag ttatttctca cctgaaccct agaggcgctt tcctgaaact aggttggcgg
     3421 ctcacagcat cacggcggaa ttgttcttgc acaaatgcaa gtcacaactt tcaactggag
     3481 ggaatgtatg aagcacctgt ctcaagattt gtttcgggtc ggtttaagcg caatcctcac
     3541 gtccgctggc ttgctggccg tcccatgctc cttgatggcc caaggctcac tgatggccca
     3601 agggcctccg acgcctgagg aacaacgggc gagggaggcc gaaagcgccg ccgacacgca
     3661 gcgggcagag cggcagcgag aagcggcgcg agcggacaag cgaaatgagc gggacgagcc
     3721 aggagaattt tacgttgccg gcttcggtgg atacaccatc ggtcatggtt ttaacgatgt
     3781 ggaggggacc ggggcgctat ccggcactaa tctcggcgac attgaccaga atgattccgg
     3841 agtatacgga gccaaggttg gctatttcat gccgcgccga ttgaattggc ttggagttga
     3901 aatggaagcc ttcaacacca cgccgcatct agaagagacc gctctgacca caggtgcaca
     3961 cctccgggtg acgaccttgg cctttaacgt gatcgcccgc gccaagatga tgtgcgagcg
     4021 gcatgacgat cacgatcgca ccggacgacc cgccggcgat cgccatgatt tctgtcggct
     4081 gcaaccgtac gccggagtcg ggttaggggt atttttcgcc cgcgcaaagg atgcaaccgg
     4141 aagtgcgtcc gacaacggcg ttccaggcct taatgcgttg gccggtgtcc ggtactactt
     4201 cactgaacgg gtggcactat tcgccgaata caagtacaac cgcgctacat ttaattttga
     4261 tgacattggc gggaccggtg ccggagtgaa aggcgactat tctgccagtc atatcgttgg
     4321 aggtctgtca ctccatttct agcaatactt ctccggtcag gatgatcgat attctggact
     4381 taggtttgaa gggcggtatt tcctgttgaa cccgttcaat tgttcttggc ggagcataaa
     4441 ggtctcccta aaaaaggtga gaactgttgt gatggttggt cgatagtggt ggaggattga
     4501 cagcgagaac catagacttt gcctcccgcc ttcctcacag tcgacgggtt ggcctcggaa
     4561 cctgcagctc gcacctgagc aatgtcgcga aatctatcca gtcagaggac ggcggtctgg
     4621 tcaaaattgc tcactaggca ataatgtatg aagagggcgg ctgttgctgt gttttcttgg
     4681 ggttgcgcct aggttctgct ggatactttc gcttgtaccc ttaatctaag gaacgaaacc
     4741 cacagaagga ggtcaatatg tatccgtttg ccacatgcgt aatcgccctc ggcatgttca
     4801 tgggcttaag cgataaccag gcttatggtc aaaccgatga ggcgtctata ttcttggttc
     4861 aaggtggaac tggcgctggg tctggtagtc aaggcatggg aactggaaaa tctggcaccg
     4921 gcaaagaaac tgacgcggga cgaggcgcgg ggaaaaagag tccgttcggc gcggaccaac
     4981 ccaccagtgg cgatttctcg tccaatccat ccaacgcgac aaccagcgac tccaagcctg
     5041 aggatttgga taagacgatc gccggttcgg aacagccagc cggaatcgga gaaaagggta
     5101 gtggacaaac acgatccact acagaacgat ccggcggtcc gcagcccagt gcgtcggaaa
     5161 gcatttcgaa atcgggcaaa gaacatggag aggggaaaac cgaacgcgct atgaaagaat
     5221 tacagtcgca aaaaccgaaa aacgagtaag cgcaaaaggc cgggcgggga tacttataaa
     5281 gttccccgcc gactcggtac ccattcttgc tatgccggtc tttccaacgg atgcaggcaa
     5341 gcttctttgg gccccgatct cgcagcggtg cgaattgcaa tcaattaacg gtcaaagccc
     5401 catgacaacg atacagatgc tactaatgtc cttggttgcc ggtgcgagca taatcagttg
     5461 cgcgatggtg gaacgtaagc tgcctcgtac tatgtccgat gcgaatatcg tatctgtgat
     5521 caatacaatc gatcagggcg aactcgacac tgcctactta gcactggtca aggcgtcagt
     5581 tcctgttcgt cagtacgctg ctcatctcgt gcacgaacat accgctagat tggacaagaa
     5641 tcggaccctc gtccatagta tgaatctgga acctgacaca gctagactct cctcggtttt
     5701 gcgggatatc aatcaagctc agatcgctgt cttgcgcaca aaatctggtt tgga
//
LOCUS       DASTQY010000261         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_621975, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000261 DASTQY010000000
VERSION     DASTQY010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621975"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VFR82_08670"
     CDS             <1..766
                     /locus_tag="VFR82_08670"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEU4501710.1"
                     /translation="AHNEAGRILPYLLQITQYFREHKRTYELLVVDDGSTDTTASVVE
                     NLGRANPAVRLLRLPRCRGKGAAVRHGMQSAVGSLQLFADADGATPIEEFARLEKALA
                     DDVDMAIGSRALASRLPGFAVQARFHRTLLGLLFNAAVRRSGIRGIADTQCGFKLFRR
                     VIAQDLFAHASVDGFGFDLEVLYVAQQRGYRIAEVPVNWSDQPGSKVRVLKDGVAMLR
                     ELATIKRNDHRGRYSLKPNSTGWGHVSADRLRIPVR"
     gene            complement(732..>2229)
                     /locus_tag="VFR82_08675"
     CDS             complement(732..>2229)
                     /locus_tag="VFR82_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501711.1"
                     /translation="FLLLMVIVNFALQPLTEPDFGWHLRTGLDLLHNGFSLPHSDPYS
                     HTMPDWAWVEHAWLTDVFIGAIYSLGGGLSIILLFACVTTGAWLIGSATSRAGVVFRR
                     LACAMSLWVALPYLGARTQLVTLLGLAVLLFLLKRWQDGVPSARWWIPPLFFGWANLH
                     GGFLAGLMVLGLIIVTTAILRWLSERRLVSARKYDEPLLSWDDLKRLTLLTILSAAVT
                     LINPYGWRLHGEIVDSLSDRFMLDTLQEWQPLSMSGVAGRRYALYLAGLTLSMMLWYR
                     RTEPVRWVIGGVFLALSLRHMRNIPFFLICSLSTCVDVMAQGFHTLQRRWPGRRLTAR
                     QGNLAAAVAMGAILFGLGADHLPQVAFSGTRPAEYFRSTSYPIEAVEWIRKNRDVIGR
                     RLYNDYAYGGFLLWWLPETRIFIDGRMPAWRRGERRIFPDYMALTGADPDLTILTKYS
                     IDWAMVRRQTQLDGALAQGPGWTRVYGDEKVAIYRLLPIELESSGDPR"
BASE COUNT          525 a          651 c          637 g          416 t
ORIGIN      
        1 agctcataac gaggctggtc gcatcctccc ttacctcctt cagatcaccc aatattttag
       61 agagcacaaa cggacgtatg agctgttggt cgttgatgac ggcagtaccg atacgacagc
      121 atcggttgtc gaaaatctcg gtcgtgccaa tccggccgtg cggttgcttc gactgccaag
      181 gtgtcggggc aagggggcgg cggtgcgaca tggaatgcaa tcagcagttg gttctctgca
      241 attgttcgcc gacgctgacg gagcgactcc tatagaggag ttcgctcgcc tcgaaaaagc
      301 cctcgccgac gatgtggaca tggcgatagg ctctcgagcg ctggcctcgc gactccccgg
      361 cttcgccgtc caggcgcggt tccatcgaac gttgcttggc ctgctgttca atgccgcagt
      421 gcgtcgcagc ggcatacgag gaatcgcaga cacacagtgt gggttcaagt tgttccgccg
      481 cgtcatcgca caagacttat ttgcccacgc ctcggtcgat ggttttggat tcgatctgga
      541 agtgttatac gtggcccaac agcggggtta tcgcatcgcc gaggtgccgg tgaattggtc
      601 tgatcagcca ggctcaaagg ttcgtgtgct caaggacggt gtcgccatgc tacgcgaact
      661 tgccacgatc aaacgcaatg atcaccgtgg gcgttatagc ctcaagccga actcgacagg
      721 atgggggcac gtcagcgcgg atcgcctgag gattccagtt cgataggcaa cagccgatag
      781 atcgcaacct tttcgtctcc atagactcgc gtccaaccag gcccctgggc cagggctcca
      841 tccaactgcg tctgtctcct caccatggcc cagtcgatcg aatattttgt gagtatggtg
      901 agatccggat ccgctccggt caatgccatg tagtccggaa atatgcggcg ctctccgcgg
      961 cgccaggcag gcatccggcc atcaataaag attcgggtct caggtagcca ccagagtaag
     1021 aatcctccat acgcgtaatc attgtataga cgcctgccta tcacatcccg gttctttcgt
     1081 atccattcaa ccgcttcgat gggataagaa gtgcttcgga aatactcggc cggcctggta
     1141 cccgaaaagg cgacttgtgg cagatgatca gcgcctaacc caaagaggat ggcacccatt
     1201 gcgaccgccg cggcgaggtt gccctgcctc gccgtcagac gacggcccgg ccagcgacgc
     1261 tggagcgtgt gaaatccttg ggccatgacg tcaacacaag tcgagaggct gcaaatcaga
     1321 aaaaatggaa tatttcgcat gtgccgcaac gagagcgcga ggaacacgcc cccaatgacc
     1381 catcggaccg gttcagtccg ccggtaccaa agcatcatgg acaacgtgag tcctgcaaga
     1441 tagagcgcat atctgcggcc ggctacaccg ctcatcgaca gaggctgcca ctcctgcagc
     1501 gtgtcgagca taaaccggtc cgacaacgaa tccacaatct caccgtgcaa gcgccagccg
     1561 tagggattga tcaacgtgac agcagccgag agaatcgtga gcaaggtcag tcgcttgaga
     1621 tcatcccagg ataacagcgg ctcatcatac ttccgcgcgg agacgaggcg tcgttcggac
     1681 aaccatctga gtattgcggt cgtgacgatg atcagtccca agaccataag ccctgccaga
     1741 aagccgccgt ggagattcgc ccagccaaaa aacaacggcg gaatccacca tcgggcagac
     1801 ggtacgccgt cctgccaacg tttgagcaga aacaagagca cggccaaccc gagcagcgtc
     1861 accaattgcg tccttgctcc gagatagggc aatgccaccc agagagacat ggcgcaggcg
     1921 agccgacgga agacaacccc ggcacgggag gttgcgctgc ctatcagcca tgcgccggtc
     1981 gtgacgcacg caaacaatag gatgatgcta aggcctccac caagcgaata gatggccccg
     2041 atgaacacgt cggtcaacca ggcatgttcg acccatgccc aatccggcat cgtatgagaa
     2101 taggggtcgc tgtggggcag cgagaatccg ttgtgcagaa ggtccaaacc ggtccggaga
     2161 tgccagccga aatcgggctc cgtcagcggt tgcagggcaa aattcacgat caccatgagg
     2221 aggaggaaa
//
LOCUS       DASTQY010000262         9082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1806054, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000262 DASTQY010000000
VERSION     DASTQY010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9082
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806054"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VFR82_08680"
     CDS             complement(<1..560)
                     /locus_tag="VFR82_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501712.1"
                     /translation="MKPSYRLRFLVLALAISPVACAAARQGATVPEPSGSPMQRVTVL
                     SYNTLHGLEPSGLTVKPSESNEARQARLHLQFEQLAVARPELILLQEVNPLPEMAETY
                     VAAMKRVGVEYSQVHQVDACGVRLAPGLAVVPYLNNGLVVLAKAPLKLRKVAGLKLSG
                     GLGRCDDYMGLQTGELRYALVAEVENP"
     gene            complement(570..1217)
                     /locus_tag="VFR82_08685"
     CDS             complement(570..1217)
                     /locus_tag="VFR82_08685"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU4501713.1"
                     /translation="MNLYSISQPIERSSQTHFTAFVSGLIVVLALNGCAGDRYTVMME
                     AAEERLNPLSAADDHASEVRLHQALVDHFGFSALGLATYVFMNRGYVVGYVDSPEQAQ
                     AVFETAKTVKGLQSIEAVLPVKQRPSADAGKVTADSVLKSQIESALTGGIGMVSGRVH
                     VEVLNGRVVLLGVVSGGEDRERAERAAAGTMGVTRVTNWLLLPDTEYMAIRSQVF"
     gene            complement(1396..1833)
                     /locus_tag="VFR82_08690"
     CDS             complement(1396..1833)
                     /locus_tag="VFR82_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501714.1"
                     /translation="MNHPFSLKKSWMTCPLVLMVSAMVACAGGPSQSDPSWKSVPITD
                     VSMVVGEWDGTVTKDEAMFPEGAVRLIIRPNSTYLFAGETLSQSAVGSGGVEPREGRL
                     IGDTEHRAVTFTLYDHKGARVLVVDTTNKENGTRYHGQFTKVR"
     gene            complement(2023..2457)
                     /locus_tag="VFR82_08695"
     CDS             complement(2023..2457)
                     /locus_tag="VFR82_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501715.1"
                     /translation="MNPLSKAVRWVRFYGGAFLVGALAMSIVRLLHDQTSWKFLDITA
                     PDMVTGTWIGTMQTDSGTLPEGALSLMIDDDLHYALRGQTASQMAVESGSLDLCGGRL
                     IGATARWVLTLALYDCRGTAVIFLQSTNRETGDRYYGEFVRS"
     gene            2791..3807
                     /locus_tag="VFR82_08700"
     CDS             2791..3807
                     /locus_tag="VFR82_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU4501716.1"
                     /translation="MRLSRRTLLQATGLGALAGMVGGCDAVGGVFGRMFAVPPRETTY
                     FTPNSKFYVVNYADSAVSVSREINIEQWKLTVKGEVKTPMSLGWRDILNRDSYDQVST
                     LMCIDTLPGGDSLGNATWRGISLKKLLQDAGVDEDTARDVVFRGIDGYDDSIPFKRAM
                     QDDVMLAFLMNGEKLPKEHGFPLRAIVPGLYGIKNVKWIHEIEVYPGDYLGYWQRKGW
                     TDDGTIKIFSRIDSPGHYQTLRGGQQTFRGIAFGGPNSIGKVELSFDSGRTWNECTIA
                     PPMSPYSWVLWSYTWQPPKPGKYQTVVRATDTKGQLQIAELVRPQPAGASGYHTIISE
                     VSEV"
     gene            3883..4032
                     /locus_tag="VFR82_08705"
     CDS             3883..4032
                     /locus_tag="VFR82_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501717.1"
                     /translation="MIRKLSSGSYRLYSRKKDSKTGKRRNLGTFKTRAAAEKHELEVQ
                     YFKRH"
     gene            complement(4072..4695)
                     /locus_tag="VFR82_08710"
     CDS             complement(4072..4695)
                     /locus_tag="VFR82_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501718.1"
                     /translation="MKRIFWVLVMTVAFAGCAVEQEYSRTPRTAVEQLLLTQAIERSF
                     HNLQLELPPGATLELQATSLDSDRTLVRINGPELASAKRPALETTYIRDAVAAVLGRH
                     GYRIPIQDASYLVRVMVESLGTMQGLTLIGLPPIQSTVIPFSLPELALFKFQKQSGYA
                     RLHVDMFDNRTGGFVGSSATIIGRTYYDQYTVLFYVTWAATDLLAPP"
     gene            4888..5712
                     /locus_tag="VFR82_08715"
     CDS             4888..5712
                     /locus_tag="VFR82_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501719.1"
                     /translation="MSEQTFTDPLASEFYRQGESELESAQSAEAVLRKAEAFGQKDAR
                     TEIMQSAFYFLAAAHFLEGRNAGDSAQAYHQAAQQLHRIGYFTQAGRAYSNAGRLAEH
                     AAQVAASPSSRHDLQHLAVRSYSRANHCFAEVGELDWSQTEYLNERNARVTWAKMEGK
                     HPWAQLAWKATSNYGTSFSRWGLWVIGTIGLFSLLYELFFQLRWLQPMETDAVAPWIP
                     FWSAVYYSVNVTAALGLVDYQPTHVLSQGVVVVNVLAGYILLGIGIGIIGRMIRTR"
     gene            complement(6243..6836)
                     /locus_tag="VFR82_08720"
     CDS             complement(6243..6836)
                     /locus_tag="VFR82_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU4501720.1"
                     /translation="MFHKPYDVLPCFKDPDGRPTLADYITVPGVYAAGRLDRDSEGLM
                     ILTSDGALVHRITDPQHKLPKVYWAQVERVPEEEALAQLRRGVMLRGIRTRPAQVRLL
                     RQEPLLWDRPVPIRVRLRVPTAWLELVIHEGKNRQVRRMTAAVGHPTLRLIRVAIGAI
                     RLDNLQPGQWRDVSSAELRTLCQQRRLDPPARPPRRR"
     gene            7169..7348
                     /locus_tag="VFR82_08725"
     CDS             7169..7348
                     /locus_tag="VFR82_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501721.1"
                     /translation="MTRFVHLIVLAALLAVGIGAPVCFSNEPAPAEQKEKKDTSGSKA
                     DDENKQDDKKGVGGK"
     gene            7685..>9082
                     /locus_tag="VFR82_08730"
     CDS             7685..>9082
                     /locus_tag="VFR82_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4501722.1"
                     /translation="MTPHSIRHHRILIQTSLITSVLLMGSGCVSTDTHQKALEELEKN
                     KKLSASQAAELDALKKKSKAESDQLQQQLAGLQHNLDEETIQRKNAQQQAASLEKERE
                     ALTARSAELQSKLDGLERDKNQLGTELSDVRGQIATLEKKLADETAQVSALESDKQQL
                     ASGTATAQSEIIKLQQRAAELEAESARVAKEREQLRQEQSRLSAALDQERQRLKTEAE
                     EKGRLAKEREQLQQEQSQLSASLDEERKRLKAEAEEKERLAKEREQLQQEQSQLSASL
                     DEERKRLKAEAEDKARLEQERAAKEDEIRRLTKTQEELSRSLKDEIAKGNITIQQVRD
                     RLTINMVDRVLFDSGRADIKPAGLKVLKQVSDVLNKISDKQIRIEGHTDNVPISTKLQ
                     DKFKTNWELSTARATIVVRYLIDQGGVQREVLSAVGYADTHPIASNDSEQGRSSNRRI
                     EIVLHPKDLSQIAGGL"
BASE COUNT         2085 a         2644 c         2525 g         1828 t
ORIGIN      
        1 gggttttcga cttcggcgac caaggcatag cgaagttcgc cggtctgcag acccatgtag
       61 tcatcgcaac gtccgagacc tccgcttagc ttgagccccg caacttttct gagtttcaaa
      121 ggagctttgg caaggaccac caacccattg ttcagatacg gaaccacagc gagccctggc
      181 gcaagacgca caccgcaggc atcaacttga tgcacttggc tatactccac accaacacgt
      241 ttcatcgcgg cgacatacgt ctcggccatc tcaggcagag gattgacctc ctgcaggagg
      301 atgagctcag gtcgggccac cgctagttgc tcgaattgca aatgaagcct cgcctggcgt
      361 gcctcattag attcgctcgg tttaaccgtc aggccgctgg gctccaggcc atgcagcgtg
      421 ttgtaggaca gcacggtgac ccgttgcatc ggcgaaccgg atggctccgg tacggttgcc
      481 ccctgtctcg ctgcagcaca agcgacgggc gagatcgcaa gggccaagac aaggaagcgg
      541 agacgataag atggcttcat caggcgctcc tagaagactt gagaccggat cgccatatac
      601 tctgtgtccg gcagcaacag ccaattggtg actcgtgtga ctcccatcgt ccccgccgcc
      661 gcccgttctg cccgttccct atcttcgccg ccagagacga cgcccaagag caccacacgg
      721 ccgttcaata cctccacatg cacccggccc gacaccatac ctataccgcc tgtgagggca
      781 gactcgatct gagacttcag aactgaatct gcagtcacct ttccagcatc cgcagatggc
      841 cgctgtttca ctggaagcac ggcctcaatg gattgaaggc ctttgacagt tttcgcagtc
      901 tcgaacaccg cctgagcctg ctcagggctg tcgacgtatc cgacgacgta tcccctgttc
      961 atgaagacat aggtcgcaag accgagggcg gagaatccga aatgatcgac cagggcttga
     1021 tgcaggcgaa cctcgcttgc atggtcgtcg gccgctgaaa gggggttcag cctctcctcc
     1081 gccgcctcca tcatgactgt atatcgatct ccggcgcagc catttagggc cagcaccacg
     1141 atcagcccac tcacaaaagc agtgaagtgg gtctgactac tccgctcaat cggttgcgaa
     1201 atgctgtaca gattcataga gatgcaccca acgcatctcg aattggatac ggcgactatc
     1261 gagatgtgtg gggtaccatg tctgggcaaa cagggaaact gtcaaaaata acaggagcag
     1321 cgtgagaccc taatggatgc ggggtggtcc ctgtgatgat agagagttac ctgcgagttc
     1381 ccataggtcg gatcactatc gaacctttgt gaattgtcca tgatatcgtg taccgttttc
     1441 tttgttggtc gtatccacga cgagcactcg cgcccctttg tggtcataaa gggtaaacgt
     1501 cacagcgcga tgctccgtgt caccgatcaa tcgcccctcg cgcggttcga ctcctccgga
     1561 acctacggcc gattgggaca aggtttcgcc ggcgaacaga taggtgctgt tggggcggat
     1621 gatcaggcga accgcaccct caggaaacat ggcctcgtcc tttgtcacgg ttccgtccca
     1681 ctcccccacc accatcgaaa catcagtaat cggcacggac ttccacgagg gatcagattg
     1741 agacggtcct ccggcgcatg cgaccattgc actgaccatg aggacgagcg ggcaggtcat
     1801 ccacgacttt ttcaggctga aagggtgatt catacagagc ctcctgcagt ctgaaggcca
     1861 ttgtcactcg ccttggatgt cttgtcatcc atgaaacgcg tccccttcgg ggcagcggct
     1921 acagtcggct gctgagcgac gaacttgcaa tatggcaggc aaccgaggct catgtgattc
     1981 atacatcgcg acaccctctg tgatcaatga ggctgacaac tgttacgatc gcacgaactc
     2041 tccataatac cggtctccgg tttcgcgatt ggtcgattgc aggaaaatga cggcagtgcc
     2101 tcgacaatca tagagtgcca gagtcaggac ccaacgggcc gtagctccta tcaaccgccc
     2161 tccacacaag tccagtgatc cgctctcaac ggccatctgt gaagcagtct gccctctaag
     2221 ggcatagtga agatcgtcat caatcatcag agatagtgcc ccctctggaa gagtgccgga
     2281 gtcagtttgc attgtgccta tccaggttcc cgtcaccata tcgggagcgg tgatgtccaa
     2341 gaatttccaa gacgtctggt cgtggagcag tcgaacaata ctcatcgcca gcgcccctac
     2401 caagaaggcg cccccgtaga accggaccca acgtaccgcc ttcgatagcg gattcatatc
     2461 atgacatttg cgtcgaagat gtggcgcgat tcgaggtcca ggaccaaccg accgtccaca
     2521 gaaccctggc agtctggcga tttcgaagcc actggcattg gtgtcgcggt cgactcacga
     2581 gttctcctta ctgggtatcc ttgggtcact gtaagccaca acgcgtgcag gagaaactgt
     2641 caaaaatgac aggcgacacg atccgcacat ggtgatggtg ttagaagccc cctccatccg
     2701 cccttcatcc gccgcagccc aatgtaagag ggcgctggag ttcatggtca ctcgttgcgg
     2761 tcccttgctt ccatgtacga taatgagcac atgcggctct cgcgacggac attgctccag
     2821 gcgacgggat tgggggcgtt ggccggcatg gtcggcggct gtgacgccgt tggcggtgtc
     2881 ttcggtcgca tgttcgcggt tccccctcgc gagacgacct atttcacacc caactccaaa
     2941 ttctatgtcg taaattatgc cgacagcgcc gtgtccgtat cgcgcgagat caacatcgag
     3001 caatggaaat tgaccgtcaa gggcgaggtc aaaaccccga tgtcactggg ctggagggac
     3061 atcctgaatc gtgattcgta cgatcaggtc tccacgctga tgtgcatcga caccttgccg
     3121 ggcggcgaca gtcttggcaa tgccacctgg cggggcattt cattgaagaa actccttcag
     3181 gacgccggcg tcgatgaaga cacggcacgg gacgtggtgt tccgcggcat cgatggatac
     3241 gacgacagca ttccctttaa gcgagcgatg caggatgacg tgatgctcgc ttttctcatg
     3301 aatggggaga agctgcccaa ggaacacggg tttccactcc gcgccatcgt gccgggccta
     3361 tacggcatca agaacgtgaa atggatccat gagatcgaag tgtatccggg cgactacctg
     3421 ggatactggc agcgcaaagg atggacggac gacggcacca tcaagatctt ttcgcgaatc
     3481 gactcgccag ggcactatca aacgctgcga ggaggccagc agacgttccg aggcattgcg
     3541 ttcggtggac ccaacagcat cggcaaggtc gaattgagtt tcgattccgg ccgcacgtgg
     3601 aacgagtgca cgatcgcccc gcccatgtca ccctattcct gggtcctgtg gagctatacc
     3661 tggcagccgc cgaagcccgg aaagtaccag accgtcgtcc gggcaactga cacgaaggga
     3721 caactgcaga ttgctgagct ggtccgacca caaccagcgg gagcgtcagg ctatcacacg
     3781 atcatctccg aggtatcgga agtctgattg tgggcgtccc gctgctctca cgcctgcttc
     3841 agtcgtgact tcgtatcgcg gatccctaga ggaccaccat ccatgatacg aaaactatcg
     3901 tcggggtcct accggctcta ttctcgcaag aaagattcca aaacaggcaa gcggcgcaac
     3961 cttggtacgt tcaagacccg ggcggcggct gagaagcatg aactggaggt gcagtatttc
     4021 aagcgccatt aattggcagt cagtagaaac ggtctgcctt tacgctgcgg cttatggtgg
     4081 agcgagcaga tccgtcgcgg cccaggttac atagaatagc actgtgtact gatcataata
     4141 agtccgtccg atgatcgttg cggacgaccc gacgaatccc cccgtgcgat tgtcaaacat
     4201 gtccacgtgc agcctggcat atccgctttg cttctggaat ttgaatagcg caagttccgg
     4261 aagcgaaaag gggatgaccg tgctctggat aggaggaagg ccgatgaggg tcaatccctg
     4321 catggttccg agcgattcca ccatcacgcg gaccagatag ctcgcatctt ggatcggtat
     4381 ccggtaccca tgccgtccca agacagcggc caccgcatcc cgaatatagg ttgtctcgag
     4441 cgcaggacgt ttcgctgaag ccaattcagg cccgttaatc cgtaccaagg tccgatcgct
     4501 gtcgaggctg gtcgcctgga gctcgagggt tgcgccggga ggcaattcca gttgtaggtt
     4561 gtggaatgac cgttcaatcg cctgtgtcaa gagcagttgc tccactgcag tcctgggggt
     4621 gcgagaatat tcttgctcca ccgcgcaccc ggcaaaggca actgtcatca cgagcaccca
     4681 gaaaattctt ttcatagggt caagcactta ggccgagaag ggtacagcct aatcgaaaga
     4741 catactcacc gcaaggcgaa atggggcggt accacaatga gagcggccct tcagatcgaa
     4801 tcgctcctac ctggcctgga tgggctttcc tgcggggtcg tctttcggtt aacatggcgt
     4861 cccacgacca ccgcaccatc gaatcagatg tctgagcaaa cttttaccga tcctctagcc
     4921 agtgaatttt accgacaggg agagagtgag ctggaatcgg cgcagtccgc ggaggcagtc
     4981 ctccgaaaag ccgaagcgtt cggacaaaag gacgccagga cggaaatcat gcaatcggcc
     5041 ttctattttc tggctgcggc gcactttctt gaagggcgaa atgcaggcga ttccgcccaa
     5101 gcttatcatc aggccgccca gcaactgcac cggatcggat acttcacgca ggcgggccgc
     5161 gcctactcca atgccggacg actggcagaa cacgccgctc aagtggcggc gagcccttca
     5221 tcgagacatg atctgcagca cttagccgtc cgctcatact cgcgtgccaa tcattgcttc
     5281 gccgaagtcg gcgagttgga ctggtcgcag accgaatatc tgaacgagcg caatgcccga
     5341 gtgacgtggg ccaagatgga ggggaaacat ccctgggctc agctggcgtg gaaagccacg
     5401 agtaactacg gcaccagctt ctcgcgttgg ggcttgtggg tcatcggcac gatcggactg
     5461 ttcagcctgt tgtacgagct gttcttccag ctacgctggc tgcaaccgat ggaaacggat
     5521 gccgtcgctc cctggattcc attctggtcg gccgtctatt attcggtgaa tgtgacggca
     5581 gcactgggat tggtggacta tcaacccacg catgtcctca gccaaggggt cgtcgttgtc
     5641 aatgtcctgg cgggatatat cctgctgggc atcggcatcg gcattatcgg ccgcatgatc
     5701 cgcacccgtt agcgctttcc cttccgttag gacaaatgat ccttccacgc ttgctcgttt
     5761 gcttcctcag aacggcgcgc atgtcgcttc tactgcgccc gtcgggggag cacctccttt
     5821 gatcccaaaa tgcgagtaat cccgtgcgcc gccctccggg agcacaggag cgacatgcgt
     5881 ggttgttcgc ccctagcgcc taagcagccc cgcgattgag atccgatatt cacagggttt
     5941 gacccgactc cgagaggtcc tccctatccg actcagtcgg tccaccgaca aaaacacctg
     6001 ggcccacccg attcccgtca aggatgacag cccttccata gtttgtgtcc caccccgttt
     6061 aagcgccagc agtatcgtcc gatctacgca ccctaaagtt gtgcattgcg cctgaattcc
     6121 catggtcccc tccgttcaac cgaaaggtga gaagcatgtg gtggctactt acgttgttca
     6181 gcgtaagtat gagccgcaaa tggcgcaatg caacgatttc gtgattttgc gcaggccctg
     6241 ggttaacgac gccgaggagg gcgtgcaggc gggtccaggc gacgctgttg gcacaaagtc
     6301 cgaagttcag cgctggatac gtcgcgccat tggcccggtt gcaggttatc gagccggatg
     6361 gcgccgatgg cgactcggat caaacgcagt gtcggatggc cgactgctgc agtcatgcgt
     6421 ctcacctgcc gattcttccc ttcatggatc acgagctcaa gccatgccgt aggcaccctc
     6481 aggcggaccc tgatcggtac cggccggtcc cataggagcg gttcctgcct gagaagacgc
     6541 acttgggcgg gtctcgtgcg aatccctctc aacatcacgc cgcggcgcaa ttgggccaag
     6601 gcttcttcct cgggaacgcg ctcaacctgg gcccaataca ccttggggag tttgtgctgc
     6661 ggatcagtga tgcgatgcac cagtgcgcca tcggaagtaa ggatcatcag cccttcgctg
     6721 tcgcggtcca agcgtcctgc ggcatagact ccgggtacgg tgatatagtc ggcgagggtt
     6781 ggccgcccat ctggatcctt aaaacagggc agaacatcgt atggcttatg aaacactata
     6841 gttcgtgaca tagctcaaca gtaatacact tgtacgcgtg gccggtgagc tcaatccccg
     6901 tcgtcatgta ggttttatgc gtttcccttc acacgtctat cgccgtccct tcatacgtat
     6961 tcccccttcg gcttactttc agagactcaa acgacccgcc tgacccattc cgaacagtga
     7021 atttccctag ctttgagccc tgagggatag acattcaata cggcatcgca attgctcacc
     7081 gcgagatcgc tgcctcgctg atcactttaa cccgaatgga tgtcaccgga tgacacccgt
     7141 tcttttccca tcccaaggag gttctatcat gacccgtttt gtacacctta tcgtattggc
     7201 ggcgctcctg gcagtgggca tcggtgcgcc ggtatgcttc tcaaatgagc ctgctccggc
     7261 ggaacagaaa gagaagaagg acacgagcgg aagcaaggcg gacgacgaga acaagcagga
     7321 cgacaagaaa ggtgtaggcg gcaagtagtc gctttccggg agcgcagcac gtgctgcgct
     7381 cccttttctc ccccctccct gcatccgatt tccagaacgc tcgcaaccgt cttgattgac
     7441 ccgcacattc agtgcaccat agggctccgt gtgactgtac gtgggtccct acaggccgtg
     7501 ttccccgtgt ccactcgggt cgaaacggtg gtgattggag cgtcgttgat tgaggccatt
     7561 gaagtcattg agtagccagc atttgttctc gctttccccc ctcatcggct catgttaaat
     7621 aagggcaccg actttgctcg tacctgattc ggcatacatc tacaccttca taacaggagg
     7681 tcttatgaca ccgcacagca ttcgccatca tcggatcctc atccagacca gcctgatcac
     7741 gtcagtcttg ctgatgggat ccggctgcgt ctcaaccgat acccaccaga aggcattaga
     7801 agaacttgag aaaaacaaga agctatcggc cagccaagca gcggaattgg acgcgctgaa
     7861 aaagaaatcc aaagccgaat cggatcaact gcaacaacaa ctggcagggc tccagcacaa
     7921 tctcgatgag gaaacgatcc agagaaaaaa cgcgcagcag caagcggctt cgctcgagaa
     7981 ggaacgcgaa gccttgaccg ccagatcagc ggaactccaa agcaagctgg acggcctcga
     8041 gcgcgacaaa aaccagttgg ggaccgagtt gagtgacgtg cgcggacaaa ttgcgactct
     8101 ggaaaagaaa ctggccgacg agaccgcgca ggtatccgcc ctcgaaagcg acaagcaaca
     8161 gctcgccagc ggaacggcca cggctcaaag cgagatcatc aagcttcaac agcgggcggc
     8221 cgaattggag gccgaatcgg cacgggtcgc gaaagagcgt gaacaattgc gccaagagca
     8281 gtccaggctt tcagctgctc tggaccagga acgccagcgt ttgaagaccg aagcagagga
     8341 aaaggggagg ctcgccaaag agcgcgagca gctccagcag gagcaatctc aactgtcggc
     8401 cagtctggac gaagagcgca agcgcctgaa ggccgaagcg gaggagaagg agaggctcgc
     8461 caaagagcgc gagcagctcc agcaggagca atctcaactg tcggccagtc tggacgagga
     8521 gcgcaagcgc ctgaaggccg aagcggaaga caaggctcgt cttgaacagg aacgagcagc
     8581 caaggaagac gagatccgtc gtctcacgaa gacccaggag gagctctcga gatccctcaa
     8641 ggacgaaatc gcgaagggaa acattacgat ccaacaggtc cgcgatcgtc tcacgatcaa
     8701 catggtcgat cgtgtgctgt tcgactccgg ccgcgccgac atcaagccgg cgggattaaa
     8761 agttctgaaa caggtcagcg acgtgttgaa taagatctcg gacaagcaaa tcagaatcga
     8821 agggcatacc gacaacgttc ctatcagcac gaagcttcag gataagttca agacgaattg
     8881 ggaactctca accgcccgtg cgacaatcgt ggtgcgctac ctcattgatc agggaggtgt
     8941 acagcgagaa gtgctctcag cggtgggata tgccgatacg catcccattg caagcaatga
     9001 ctcagagcag gggcgctcct ccaaccggcg catcgagatc gtgcttcacc cgaaagatct
     9061 ctcgcaaatc gcgggcggcc tg
//
LOCUS       DASTQY010000263         6964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_969638, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000263 DASTQY010000000
VERSION     DASTQY010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969638"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VFR82_08735"
     CDS             complement(<1..1070)
                     /locus_tag="VFR82_08735"
                     /inference="COORDINATES: protein motif:HMM:NF039196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEU4501723.1"
                     /translation="MKHQNDEQETTALEDAPVAANGGTRRRHDINEQLGEILNVMVAY
                     RNGDFSVRLPATWTGVLGKMSDTMNDILSVSQRRAEEVVRISRVVGKEGRLRQRLSVP
                     GVTGGWSDEIESLNTLIDDLASPTTEVTRAVGAVGKGDLSQAMALEKDGRPLEGEFLR
                     SAKLVNKMIEQLAVFTSEVTRVAREVGTEGKLGGQAQVKGASGVWKELTDSVNQMAGN
                     LTAQVRNIAEVTIAVANGDLSKKITVDVRGEILQLKEAINTMVEQLRSFASEVTRVAR
                     EVGTEGKLGGQAVVPGVAGTWKDLTDNVNSMASNLTAQVRNIVEVTVAVARGDLSRKI
                     TVDVRGEILQLKETINTMVEQLR"
     gene            complement(1545..2534)
                     /locus_tag="VFR82_08740"
     CDS             complement(1545..2534)
                     /locus_tag="VFR82_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501724.1"
                     /translation="MRSTPLCLVAVSSLLLTACASTLPPGKATSFDRRCLPASMDLRR
                     MPLSEMDKPAATSLSAERQEAVKLYSTIAVHVADVMDLLPLLNHLARLENQHASSVEI
                     ERARRKLTTRLQLANMEVSSLVAEIECEVQRADEVQDRLKQVQTTRTTTQTILGIIAG
                     GLANILSGGIGMATRAGDAADIASVAGGTLEVLFGTSANFTKVRQEFTHPHNHLQAFW
                     NGEGKEKEFFPPGIWRFLTEPDIRDLEGHSLRDVLIQTWNEGRLGTPGSHQEKERKAL
                     LFGEGGLYDSEDLHVREAMLHQLESSIQLMHQDLETLLREVLLRQALEEDGVS"
     gene            complement(2553..3686)
                     /locus_tag="VFR82_08745"
     CDS             complement(2553..3686)
                     /locus_tag="VFR82_08745"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501725.1"
                     /translation="MSERERTIGRKSVQSPHIGGHAVADGKSVARSAGLHYVDDRRPG
                     IARVRQRHGMRYLWPDGRPVRDGETLTRIRSLAVPPAWRDVWICLDADGHVQATGRDA
                     RGRKQYRYHPRWREVRDGNKYGRMIAFGRALPLIRKRVEKDLARKGLPREKILATVVR
                     LLEVTLIRVGNEEYAKKNDSFGLATMRDRHVRVNGSALRFQFHGKSGILHAVDLQDRR
                     LASIVRQSRDLPGFELFQYLDETGQRRTIDAADVNAYLKTIAGDEFTAKDFRTWAGTV
                     LAAYALREFSAVDSQAQAKRNVLRAIETVAKRLGNTKAVCRKCYVHPDIVNAYFDGTL
                     AASLNRRTGRELRSHVRDLPPEEAAVLALLHRRLGQDARRAAA"
     gene            complement(3800..4666)
                     /locus_tag="VFR82_08750"
     CDS             complement(3800..4666)
                     /locus_tag="VFR82_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEU4501726.1"
                     /translation="MPRVVWKGAISFGLVHIPVGLYSAEKRNNFDLTMLDRRNMKPVG
                     FKRYNKENGKEVTWDDIVKGYEYEKDRYVVLTDEDFRRANVEATQTVDILTFVEQEEI
                     EPIYFETPYYLAPDKRGHKGYALLRETLKQTGKTGIANVVIRTRQYVAALIPVGDVIV
                     MNTLRYSNELRDTAELELPSSNLKAVGVNPREIEMARRLVEGMSAAWKPQEYKDSYHE
                     DLLALIEKRIKAGQTEVVMEPAPEEKKRSAKGEVIDLMALLKKSVESKGKARMPAAPA
                     KARGRKSGRRAA"
     gene            complement(4755..5687)
                     /locus_tag="VFR82_08755"
     CDS             complement(4755..5687)
                     /locus_tag="VFR82_08755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU4501727.1"
                     /translation="MSRTAGASTAIRRTVSHPWTLGGMSLAELGRRLWRKSQEHELLG
                     RAAQLSYYALLALFPALIFLTALMGLFSVQSFMPELMSYLRNVLPEDALSMVERFLTQ
                     VAEGSGANILSLGALGALWASSSGVTAIMDALNVVFGVKEDRRQFWRVRLTALVLTIG
                     LAGFVILSMALVLYGPTIGRWIANLFGLGVAFTWLWNVLQWPIIVGLMLVVVAAIYHI
                     CPDTREKRWHWITPGSVFAVCMWLIVSLGFKAYVDNFGDYNKVYGSIAGVIVLMLWFY
                     WSGMVLLLGGEINAEIAQASREREGHKGEVRQAA"
     gene            complement(5687..>6964)
                     /locus_tag="VFR82_08760"
     CDS             complement(5687..>6964)
                     /locus_tag="VFR82_08760"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEU4501728.1"
                     /translation="KIGAELRVEEPVFVVTQKHVDGFLRKGDKEVVKEAAEAWQDRLR
                     ESMAFRGAFYVTNGHMTYDAGKAASEPLRVERFDIEATNITNRPEENDEYPAKLRVHA
                     NFPDQSQINLEGRADWFAIPVPKVDADLKIERLQLKNLLPVAGRFNVQCREGVLDVNG
                     HVQYSETSSVVAINQFQLDDAKIDYVHSAVTKPKEVQRVKKAAETVKEAQHGSTMRVK
                     VEQGKIRNSEIGFVNKATSPDYRVFLTDMEVELQNLSNRLEEGTGVVKITGKFMGSGP
                     TVMNATFRPEKPRPDFSLNAKIVKTNVSAFNDVLRAHGDMDIHQGTFAFFSEMTVKDN
                     QIRGYVKPMLKDVEVYDAEQDKDKALSKKVWEAVAGGVLGIFENKPRDEAATVTNLSG
                     PVENPQANTWQVVAKLVQNAFFKAILPGFERVA"
BASE COUNT         1430 a         2085 c         1868 g         1581 t
ORIGIN      
        1 cgcagctgct ccaccatcgt attaatcgtt tctttgagtt gaagaatttc cccccgcacg
       61 tccacggtaa ttttgcggga gaggtcgcct cgggcgacgg caacagtgac ttcgacgatg
      121 tttcgaactt gggcggtgag gtttgacgcc atcgagttca cgttgtcggt caggtccttc
      181 caggtgccgg cgacaccggg cacgaccgcc tgcccgccga gtttgccttc ggtgcccacc
      241 tcacgcgcga cacgggtcac ttccgaggca aaggaccgca gctgctccac catcgtatta
      301 atggcttctt tcaattggag gatttcgccc cggacgtcga cggtaatttt cttcgagagg
      361 tcgccgttgg ccacggcgat ggtgacttcc gcgatatttc gcacttgtgc ggtcagattg
      421 ccggccattt gattgaccga atcggtgagc tctttccaca cgccgctggc acctttgact
      481 tgggcctggc cgccgagctt gccttccgtc ccgacttcgc gtgcgacgcg tgtgacctcg
      541 gaagtaaaga ccgccagctg ctcgatcatc ttattgacga gttttgcact gcgaaggaat
      601 tcgccttcga gcggccgccc gtctttctcc agtgccatag cctgactgag gtcccctttt
      661 ccgactgcgc cgacggcacg ggtgacctcg gtcgttggag atgcgaggtc gtcgattaac
      721 gtgttcagtg actcgatctc atccgaccac cctccagtga caccaggcac cgacagccgc
      781 tgccgcaacc gcccttcctt cccgacaacg cgagaaatac ggacgacttc ttctgcccgc
      841 ctttggctga cagaaagaat atcgttcatc gtgtcggaca ttttcccgag cacacccgtc
      901 cacgtggccg gcaaccgcac ggagaaatct ccgttccggt acgccaccat gacgttaaga
      961 atttcgccaa gttgctcgtt gatgtcatga cggcggcggg taccaccatt ggcggcaacc
     1021 ggcgcatcct cgagagccgt ggtttcttgc tcatcgttct ggtgtttcat cgtgcctccc
     1081 ggcgggcaaa atcggtagaa cggtaacccg ttggtctata tgaaccgagg caacgtaccg
     1141 cgagggaaga gaccttgcta actagggaga cccctagtac ctaagtgcat acagtcatgc
     1201 acgttctacc ccgccgcggg acgcgtaaag tacaagtcag tacagcagga ttttcaccgt
     1261 cgcaagcaag agggacagct agccgtaacc cgagaaaccg tccccagccc tatacatgga
     1321 atgagctgtt tcgctgtaca gggtgggggt tgcaatgtcc ttcgagcttt caaagacttg
     1381 catcgaagag agaaggcagg ttgagtcagc atcaacgaag tccgggccat aggttggcgg
     1441 cgacactgag atttcaatcg agagcaagcg gacaccacat ggcgtttgga atggaaaact
     1501 gaaaacaaga ccgttgtccc tgcaacgcga ccaggatcga ctgttcacga aaccccgtct
     1561 tcctccaacg cttggcgaag cagaacttca cgcagcaacg tttccaggtc ttgatgcatc
     1621 aactgaatgc tggattccaa ttgatgcagc atcgcctcgc gcacatgcaa atcttcgctg
     1681 tcatagagcc cgccttctcc gaataggaga gccttgcgct ccttctcctg atgcgaaccg
     1741 ggcgttccca atcgtccttc gttccacgtc tgaataagca catcgcgcag gctgtgcccc
     1801 tccagatcac ggatgtccgg ttcagtcagg aaccgccaga tgccgggagg aaaaaattcc
     1861 ttttccttgc cctcgccgtt ccaaaatgcc tggagatgat tgtgggggtg ggtgaattcc
     1921 tgccggacct tcgtgaaatt cgcggacgta ccgaaaagaa cttccaatgt cccgccggcg
     1981 acggatgcga tgtctgcggc atccccggct cttgttgcca tgccgatacc tccagacagg
     2041 atgttcgcta atccgcccgc gatgatgccg agaatcgtct gggtcgtcgt tcgtgtggtc
     2101 tgcacttgct tgaggcggtc ttgtacctca tcagcacgtt gaacttcaca ttctatctcg
     2161 gcgactagac tcgacacttc catgttggcg agctggagcc gtgtcgtcaa ttttcgacgg
     2221 gcccgttcga tctctacaga ggaggcatgc tgattttcca gacgcgcgag atggttgagc
     2281 agcggcagca gatccatgac gtcggccaca tgcacggcga tggtcgagta gagtttgacg
     2341 gcctcttgcc gttcggccga caggctggtt gcagccggct tatccatttc gctgagcggc
     2401 atgcggcgaa gatccatcga agccggcaga cagcggcgat caaacgaggt cgctttcccc
     2461 ggtggcaatg tcgaggcaca ggcggtgagg agaagagaag aaaccgcgac cagacagagc
     2521 ggggtggatc gcatggcgcg tcgatcgctc cttcatgccg ccgcccggcg agcgtcctgc
     2581 cccaaccggc gatgaagcag ggccagcacc gctgcttctt ccggcggaag gtcccgcacg
     2641 tgtgagcgaa gctcccggcc ggttcgtcga ttgagcgacg ccgcgagcgt cccgtcgaaa
     2701 tacgcgttga cgatgtcggg atggacatag cacttccggc agacggcctt ggtgttgcca
     2761 aggcgtttgg ctacggtttc gatggcccta agcacgttac gtttcgcttg cgcctgggaa
     2821 tcaaccgcac tgaattcccg gagcgcgtaa gccgcaagga cggtgccggc ccacgtccga
     2881 aaatctttgg cggtgaactc atcgcctgcg atggtcttca aataggcatt gacgtcggcc
     2941 gcgtcgatcg tccgtctctg cccagtctca tcgaggtact gaaatagttc gaatccagga
     3001 aggtcccggc tttggcgcac aattgacgcc aagcggcgat cctgcagatc gacggcatga
     3061 aggatgccgc ttttgccgtg gaactgaaac cgaagggcgg aaccgttgac ccggacatga
     3121 cggtcacgca tggtggcgag accgaatgaa tcattcttct tggcgtattc ctcgttcccg
     3181 acgcgaataa gcgtgacctc caacaaacgc acgacggtgg cgaggatctt ctctctggga
     3241 aggccttttc gagcgaggtc cttctccacg cgttttcgga tcagaggaag cgcgcgcccg
     3301 aatgcgatca tccgtccata tttgtttccg tctcggactt cacgccatcg tggatggtac
     3361 cgatattgtt tgcggccccg cgcatcccgg cccgtcgcct gtacgtgccc gtcggcgtct
     3421 agacaaatcc agacatcccg ccaggcggga ggtacggcaa gggaacggat cctggtcaac
     3481 gtttccccgt cgcggaccgg ccggccgtcc ggccacagat accgcatacc atgtcgctgc
     3541 cggaccctcg caatgcccgg tcggcgatca tccacgtaat gcaaaccggc ggagcgggct
     3601 acagattttc cgtcggcaac ggcgtgccct ccgatatgtg gactctgaac ggactttcgc
     3661 ccgatggtgc gctccctctc gctcatatct caaaaactgg gattcgccat ggccataccc
     3721 ccttttacat ggcgaaacgg acatttatct atggccgctc accgacgttt cggtcttcaa
     3781 tggcttatcc atcatattat taagccgctc gacggcctga cttccgcccg cgggctttgg
     3841 ctggtgccgc cggcatgcga gctttccctt tggactcgac actcttcttc aaaagagcca
     3901 tgagatcgat gacttcccct tttgccgacc gcttcttttc ctcgggtgcc ggttccatca
     3961 cgacttcggt ttgtccagcc ttaatgcgct tctcgattaa tgccaagaga tcttcatgat
     4021 acgagtcctt gtattcctga ggtttccagg cggcgctcat gccttcaacc agccggcgag
     4081 ccatctcgat ttcgcgagga ttgacaccga ctgccttgag attactcgac ggcagctcaa
     4141 gctcagccgt atcccgcaac tcgttactgt agcgaagcgt gttcatgaca atcacgtcac
     4201 ctactggaat gagagcggca acgtactgcc tggtgcggat gacgacgttc gcaatgccgg
     4261 tcttcccggt ttgcttcagc gtttcgcgta acagcgcata tcctttgtgc ccacgtttgt
     4321 ccggcgccag ataatagggc gtctcaaaat atatcggttc gatctcttcc tgttcgacaa
     4381 aggtcaggat gtcgacagtc tgcgtggcct ccacgttcgc gcgcctgaaa tcttcatccg
     4441 tcagcaccac gtaccggtcc ttttcgtatt cgtatccttt aacgatatcg tcccatgtca
     4501 cctctttgcc gttctctttg ttgtaccgct tgaatccgac cggtttcata ttgcgacggt
     4561 caagcatggt gaggtcaaaa ttattgcgtt tctcggcgga gtacagtccc accgggatat
     4621 gaaccaatcc gaaactaatc gcacctttcc agactacccg cggcataagc actccctatt
     4681 ataattggca gaatctacga tcccgcccgc tgagtgatcc attcacgtct cggaagcgtg
     4741 aatcattcaa cgcattaggc tgcctgcctc acctcacctt tgtgcccttc ccgttccctc
     4801 gacgcctgtg caatctccgc gttgatttca ccgccaagca acagcaccat accgctccaa
     4861 tagaaccaga gcatcaggac aatgaccccc gcgatcgagc cgtatacttt gttgtaatcg
     4921 ccaaaattgt cgacatacgc tttgaaaccg agagatacga tcagccacat acagacggcg
     4981 aacacggaac caggcgtgat ccaatgccag cgtttctctc gggtgtccgg gcagatgtga
     5041 tagatcgcgg cgacgactac gagcatgagg ccgacgatga tcggccactg caagacattc
     5101 cacaaccagg tgaaggccac acccagcccg aacaggttcg cgatccagcg accgatcgtc
     5161 ggcccgtaca ggacgagtgc catcgagaga atgacgaaac cagcaagacc tatggtgagc
     5221 accagcgcgg tcagtcggac acgccaaaac tgacgacggt cctccttcac accaaatacg
     5281 acgttgagcg cgtccatgat cgccgtgaca ccgctggacg atgcccacaa agctcccaac
     5341 gcgccgaggg agaggatgtt ggccccgctg ccttctgcga cctgggtcag gaatcgctcg
     5401 accatggaga gcgcatcctc gggcagcacg ttacgcaaat agctcatcag ttcaggcatg
     5461 aagctctgga ctgaaaagag gcccatcaga gccgtcaaga aaattaaggc cggaaacagt
     5521 gcaagcaggg cgtaatagga gagttgggcg gcgcggccaa gcagttcgtg ttcttggctc
     5581 ttgcgccaga gccgccgacc aagttcggcg agactcatac cgcccagcgt ccatgggtgg
     5641 gaaaccgtgc gacggatagc ggtcgacgcg ccggcagtgc gactcattat gcgactcgtt
     5701 cgaagccggg gaggatcgct ttgaagaagg cgttttgcac caacttcgcg acgacctgcc
     5761 acgtattggc ctgcggattt tcaaccggtc ccgacagatt tgtgacggtg gctgcttcat
     5821 cacgaggttt attttcgaag atgccgagca cgccgccggc gaccgcttcc cacacctttt
     5881 tcgacaaggc tttatcctta tcctgttcgg catcatagac ttcaacgtct ttgagcatcg
     5941 gctttacata gccgcggatc tgattatctt tcaccgtcat ctcactgaag aatgcaaacg
     6001 tgccttggtg gatatccata tccccatgcg cacgcaggac gtcattgaag gcgctgacat
     6061 ttgttttgac gattttggcg ttgagactga agtccggccg gggcttctcc ggacggaacg
     6121 tggcattcat cacggtggga ccgctcccca tgaatttgcc cgtgatcttg acgactcctg
     6181 ttccttcttc gagccgatta ctgaggtttt gtagttcgac ctccatgtcg gtgaggaaga
     6241 cacgatagtc cggcgacgtg gccttattga cgaagccgat ttcgctgttg cgaatcttcc
     6301 cctgctcgac tttgacccgc attgttgaac cgtgttgcgc ttctttaacg gtttccgccg
     6361 ccttcttcac ccgttggacc tctttcggct tcgtcacggc tgagtgcaca taatcaattt
     6421 tggcatcgtc caactggaac tgattgatgg ccacgacgga ggacgtttcc gaatattgca
     6481 cgtggccgtt cacatcaagc actccctcac gacattgcac gttgaagcgt ccagccaccg
     6541 gcagcaagtt cttgagttgc agccgctcga ttttcagatc cgcatccact tttggaactg
     6601 ggatggcgaa ccaatcggca cggccctcaa gattaatctg tgattggtcg gggaagttcg
     6661 catgcacacg gagcttcgca ggatattcgt cgttctcttc cggtctgttg gtaatattcg
     6721 tcgcttcgat atcaaatcgt tcgacgcgga gcggttcaga agccgccttg cccgcatcat
     6781 aggtcatatg accgttcgtc acgtaaaacg cgccgcgaaa ggccatgctc tcccgtaagc
     6841 ggtcttgcca ggcctccgcc gcctccttca ccacttcttt gtcccctttc cgtaaaaatc
     6901 cgtccacatg cttttgggtg acgacaaaca ctggttcctc cacacgaagc tcggccccga
     6961 tttt
//
LOCUS       DASTQY010000264         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1034510, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000264 DASTQY010000000
VERSION     DASTQY010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034510"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VFR82_08765"
     CDS             <1..446
                     /locus_tag="VFR82_08765"
                     /inference="COORDINATES: protein motif:HMM:NF033083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAC(3)-I family aminoglycoside
                     N-acetyltransferase"
                     /protein_id="HEU4501729.1"
                     /translation="DLQRMRDLLAVFAEAFDEPNTYLSAQPGDSYLRNLLNQNHFIAV
                     TAVNGDEVVGGLTAYVLDKFEQERSEIYIYDLAVAKAHRRKGMATALIRFLQNIAKSY
                     NAWVIYVQADTSDKSAMTLYASLGRREDVHHFDIPVRIGLARPDV"
     gene            607..852
                     /locus_tag="VFR82_08770"
     CDS             607..852
                     /locus_tag="VFR82_08770"
                     /inference="COORDINATES: protein motif:HMM:NF022397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HEU4501730.1"
                     /translation="MENQVGDQPVLNYIQRLVGEEQRLYQQGKLPQADRKRLAEVQVE
                     LDQCWDLLRQRRALRDVGLDPEDAQVRPPQVVENYEQ"
     gene            complement(993..1502)
                     /locus_tag="VFR82_08775"
     CDS             complement(993..1502)
                     /locus_tag="VFR82_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4501731.1"
                     /translation="MPRRPRLAAGDLAYHVLNRRVGRLGLFEKPTSKEGIVLDHIFLT
                     VGDIELSIAFYSAALQPLGITQRLDYDGKDGPPGHPDLKGFGAQGRMFFWLRAGQPSP
                     GAVHIGFVADSEAMVNATHAAAIAAGGRQIHPPGPQLHYDPRYYAAQVADPDGYSLEF
                     VFKSWQHGS"
     gene            complement(1570..2574)
                     /locus_tag="VFR82_08780"
     CDS             complement(1570..2574)
                     /locus_tag="VFR82_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HEU4501732.1"
                     /translation="MWTLLAAAGTVLLILTTPAYVGAQKESGSQKSQAEQLVDAFNKV
                     FGAHPGIRANHSKGVVLDGAFTPSGAAASLSKAAHLQKRKTPIPVTVRFSAGSGVPTV
                     PDTDAMPRGMAVKFSLPDGTKTDLVVLSVNSFPVATADEFRDFLLAIAASGPEASKPT
                     EFEQFLGAHPAAKAFVETPKPPPVSYATLPYFGINAFKFTNAKGTHTHIRYQLQPVAG
                     AQYLPKEQVSAMGPDYLTTEIHERVRRGPIKFKLLAQVAEADDKVDDPTIVWPDTRKK
                     VELGTITITKAVADSQAAEKKLLFMPGSVVPGIEAADPMIAARSASYIVSLSRRAQAQ
                     "
BASE COUNT          552 a          752 c          780 g          571 t
ORIGIN      
        1 atgaccttca acgcatgaga gatcttctcg cagtcttcgc ggaagccttc gacgaaccta
       61 acacatacct ttcagcccaa cctggcgact catacctgcg gaacctcctg aatcagaacc
      121 atttcatcgc cgtcaccgca gtgaatggcg atgaggtcgt cggtggctta acagcttatg
      181 tgctcgacaa attcgaacag gagcgcagcg aaatctacat atatgatttg gctgtggcga
      241 aggcgcaccg gcgaaaaggc atggccactg ccctcattcg gttcttgcag aatattgcga
      301 agtcttacaa cgcgtgggtg atctacgttc aggccgatac gagtgacaaa tcggcgatga
      361 cactgtacgc ctccctggga cgtagggagg acgttcacca ttttgacatt ccggtcagaa
      421 tagggttggc acgtcccgac gtctagggtg agagttggct cgccacgcgc cgcgaaattc
      481 agaatgtccc gcttccctgt tcactgggta catcatgttg ctcagagctt cggttgaccc
      541 cagtttcggc ggagcgtaac ataaaatcgt gccgttccga tcatatccgc gtgagaggag
      601 actctcgtgg agaatcaagt cggggatcag ccagtcctca attatattca gcgccttgtc
      661 ggagaggaac aacgattgta ccagcaaggg aagcttcccc aagccgatcg taagcggctc
      721 gcggaagtcc aagtcgaact ggatcagtgc tgggacctct tgcgccagcg acgtgccctt
      781 cgcgatgtcg ggctcgaccc ggaggacgcg caagttcgcc cgccgcaggt cgtcgagaat
      841 tacgagcagt agggattcct cgtcaacgag cgcacacagc cggacaatct ggcccgccac
      901 gcctcgcgaa atggttggtg gcaccgcttc gaaactcttc aagcacttag aaaagtagcc
      961 tgtccccttt tttatcctct tttttatccc gctcaactcc cgtgttgcca gctcttgaag
     1021 acaaactcga gggagtagcc atcaggatct gcgacctgcg ccgcatagta gcggggatcg
     1081 tagtgaagct gcggacccgg cggatgtatc tggcgaccgc ccgccgcgat ggcggcggcg
     1141 tgcgtggcat tgaccatcgc ctcagagtcg gcgacgaacc cgatgtgaac cgcccccggc
     1201 gaaggctggc ccgccctgag ccagaagaac atgcgcccct gtgccccgaa gcccttgagg
     1261 tccggatggc cgggagggcc gtccttcccg tcgtaatcca agcgctgcgt gatgccgagt
     1321 ggctgtaggg cggcactgta gaaagcgatc gaaagttcga tgtcgccgac cgtcaggaag
     1381 atgtgatcga gcacgatgcc ctccttagac gtcggcttct cgaacaaccc gagtcggccg
     1441 acacgccgat tcaagacatg ataagcgagg tccccagctg cgaggcgagg ccggcgtggc
     1501 atggcagggc ttgtttcgaa accagaattt tgctggcaag aaaagactcc cgaccccttg
     1561 tctggtgcct cattgggctt gagcgcggcg tgacaacgag acaatgtatg acgccgaccg
     1621 cgcggcgatc atcggatcgg ctgcctcgat cccgggaacc acagatccgg gcataaaaag
     1681 gagtttcttt tctgcggctt ggctgtctgc caccgccttg gtgatggtga tcgtgcccaa
     1741 ttctactttt tttcgggtat cgggccatac gatcgtggga tcgtcgacct tgtcgtccgc
     1801 ctcggccact tgcgccaaca gtttgaattt gatcggcccg cgccgcactc tttcgtgaat
     1861 ctctgtggtc agataatcgg ggcccatggc cgacacctgc tctttgggca ggtattgcgc
     1921 accggcgacc ggttgcagtt gataacgaat gtgggtgtga gtcccctttg cgtttgtgaa
     1981 cttgaaggca ttgatcccga aatagggaag ggtggcgtaa cttaccggtg ggggcttagg
     2041 tgtctccacg aaggctttcg cagccgggtg ggcgccgaga aattgttcga actcagtagg
     2101 ttttgacgcc tctggtccgc tcgcagcaat cgcgagcagg aagtccctga actcgtccgc
     2161 agtcgcaacg ggaaagctgt tgacagacag gacaacgaga tccgtcttgg tcccatcggg
     2221 aagagaaaat ttcactgcca tgccgcgtgg cattgcgtct gtgtcaggaa cggtcggcac
     2281 tcctgagcct gcggagaaac gcacggtaac gggaattggg gtttttcgct tctggaggtg
     2341 agccgccttg ctcaaggatg ccgccgcccc actcggcgtg aaagccccat ccagtacgac
     2401 gcccttcgag tgattagccc gtatgccggg atgagcgccg aagaccttgt tgaacgcatc
     2461 gacgagttgt tcagcctggg atttctgact accggactcc ttctgagccc ccacatacgc
     2521 gggagttgta aggatgagaa gaactgttcc tgccgctgcg agtaatgtcc acatcgtggt
     2581 tcctcctgag agctgcattc gagtctgccg gtcgctgagt tgggaagcgg tggaaactct
     2641 actccttcat accag
//
LOCUS       DASTQY010000265         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1025537, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000265 DASTQY010000000
VERSION     DASTQY010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025537"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..404
                     /locus_tag="VFR82_08785"
     tRNA            316..404
                     /locus_tag="VFR82_08785"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Leu,seq:taa)
     gene            complement(449..823)
                     /locus_tag="VFR82_08790"
     CDS             complement(449..823)
                     /locus_tag="VFR82_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501733.1"
                     /translation="MAIGKQQACWATPTTTINCVLVRVDRQIEAEGCVLKVSESSSQL
                     TAPDFLVPGDFVKVRLWLEGENTSIHIGLAEVRRIQNHWITLEVVQVSRQERSRLQRY
                     VESRQTMLRNQPVFIDHLLIRA"
     gene            921..1304
                     /locus_tag="VFR82_08795"
     CDS             921..1304
                     /locus_tag="VFR82_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEU4501734.1"
                     /translation="MTSRRFFVLGAFFAGTAVAAGAFGAHLLKPVLDAPMLAAFETAA
                     RYQMYHALGLCLVSSADGRYPNLNCTTVGWLFTTGILLFSGSLYALSLVGIPWLGALT
                     PFGGAAFMAGWVLLAWGVLRSDRPS"
     gene            complement(1307..2218)
                     /locus_tag="VFR82_08800"
     CDS             complement(1307..2218)
                     /locus_tag="VFR82_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501735.1"
                     /translation="MLVSVTSRSLQALRALLMIACFVLVPTFLSAASEERASSSPPAW
                     HKFINLHHDRLAQVETDQAALDLFVAAIGRAAEVNDVAATIAAKGLSPQVTKDLLVPE
                     ITKSAQRLVAALVAWDIGDRMMRGLEEKSARTDVLTTPSQVAWLTANGPFTSLQEVVR
                     QWDSGDATAAAGVAAGQLAQDAAQRTMEEWWRLKTWKDRVRTARGQSRLCGTWLWVVH
                     NHQRHHEERKLSLVFPPPGMGTAIPGLTETVVLGDAVYLRWETEGRIQEDSLLFSKEG
                     QRLEGSFVNSQGGWGSITGKRTATCTP"
     gene            complement(2256..>2650)
                     /locus_tag="VFR82_08805"
     CDS             complement(2256..>2650)
                     /locus_tag="VFR82_08805"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU4501736.1"
                     /translation="TREFIKRNIHGVVGYGMGGTLAMKFACQRKRLRGAVSYYGRMIQ
                     PDSALENALCPILYHYAEQDPWVSGQDVQRLRELAAKYNKPIAIQSYSNAPHAFSDET
                     RPESYREAAATEAWATTAMFLKKHLQGS"
BASE COUNT          519 a          779 c          763 g          589 t
ORIGIN      
        1 ggttgagccg ggcggttcag tcgggagtga aggaagcaat ggaccgcacg aagcggcctc
       61 tggtcgaagc tgtctaggtc cctcattctt cagatccgca aataaccacc gacgcattca
      121 gtctgtcgcc gcagtggcta caggctgttt gcgtgcgttg tgctccttga ccgtcctcca
      181 cctcgatgct agagtccgcc tgctccctca ttcagtcggt tagtccaaac agctctccag
      241 gccagcctga acctgtccgg ctctcggtcg acagacagca ggtgagcgta aggctccgct
      301 tgtgtgactg atcctgcagg agtggcgaaa ctggcatacg cacaggactt aaaatcctgt
      361 ggcccgaaag ggccgtacgg gttcgatccc cgtctcctgc accagcgctc cggtgtttca
      421 gcgcgacttc gttccgtttg aaggcggatc aggcccgaat gaggagatgg tcgataaaga
      481 caggctgatt gcggagcatc gtttggcgtg actcgacgta tcgctgcaac cggcttcgtt
      541 cttgccggct gacttgcacc acctcgaggg tgatccaatg attctggatt cgcctcacct
      601 cggcaagccc gatgtggata ctagtgtttt ccccttccag ccacagacgc actttgacaa
      661 agtcgcctgg aaccaggaag tcaggggccg tgagctggga actggactcg gagaccttca
      721 gcacacaccc ctcggcttca atttggcggt ccacacggac cagcacgcaa ttgatcgtcg
      781 tcgtgggagt ggcccaacaa gcctgttgtt tacctatagc cataggctga ggctagccta
      841 gagccgatga ggcgtggttg gctatccctc aagaggaggg gatagatgga ctgtaagatg
      901 aaaggtcagg cttgaagaac atgacatccc gccggttttt cgttctggga gcgttctttg
      961 cgggtacggc cgtcgcggct ggtgcctttg gcgcgcacct gttgaaaccc gttctggatg
     1021 cgccgatgtt agccgctttc gaaaccgccg cacgctacca gatgtaccat gccttgggac
     1081 tctgcctcgt ctcatcggca gacggacgct accccaactt gaactgcacg acggtggggt
     1141 ggctttttac aacaggaatc ctgctgtttt ccggaagtct atacgctctc tcgctggtcg
     1201 gaattccatg gctgggtgcg ctcacaccct tcggtggagc cgcctttatg gcaggctggg
     1261 tcttattggc gtggggagtc ttgcgttccg atcggccttc ctaacgttat ggcgtgcaag
     1321 tcgccgtgcg ctttcctgta atggaacccc acccaccttg ggaattgacg aaactccctt
     1381 ccaaccgctg accctctttg gaaaaaagca ggctgtcctc ctgaatccgt ccctctgttt
     1441 cccatcggag atagacggca tcacccagta cgaccgtttc agtgagacca gggatagccg
     1501 tgcccatccc gggtggcggg aacacgagcg acagcttacg ctcttcgtga tgccgctgat
     1561 ggttgtggac gacccaaagc catgtcccgc agaggcgaga ctgtccgcgc gccgtgcgca
     1621 cgcgatcttt ccacgtttta agccgccacc actcttccat cgttcgttga gcggcgtctt
     1681 gcgccagctg tccggccgca acgccggcgg cggccgtcgc gtccccgcta tcccattgcc
     1741 tcaccacctc ctgcaaagat gtaaatgggc cgtttgccgt cagccatgca acttgggatg
     1801 gcgtcgtgag gacatcggta cgggcgcttt tctcttccaa accccgcatc atccggtccc
     1861 cgatgtccca ggcaaccaac gccgcaacca atcgctgcgc ggatttcgtg atttctggca
     1921 ccagcagatc tttggtgact tgtggagaga gtcccttggc ggcgatcgtc gccgccacgt
     1981 cgttcacctc agcagctcgg ccgatcgcag cgacgaacag gtcgagtgcc gcttgatcag
     2041 tttcaacttg agcaagccga tcatggtgca ggttgatgaa cttgtgccaa gccggcggag
     2101 acgaggaagc gcgttcctca gaggcagcgg aaaggaaagt cgggacgagg acgaagcacg
     2161 cgatcatgag caacgcccgt aaggcttgca gactgcgcga tgtgacggag acgagcatgg
     2221 tgacggacag atgattcgcg gcagagggac ggtcgttatg atccttgaag gtgctttttc
     2281 aagaacatgg cggttgtcgc ccatgcttca gttgctgcgg cttcccggta gctctcaggt
     2341 ctcgtttcgt cactgaaagc atgcggcgca ttggagtagg actgaattgc tatcggcttg
     2401 ttgtatttgg cagcgagctc ccgcagacgt tgaacatcct gtcctgacac ccagggatcc
     2461 tgttccgcat aatgatagag aatgggacag agcgcgtttt ccaaagcgga gtccggctga
     2521 atcatgcgtc cataataaga gaccgctccc cgcaggcgct tccgctgaca ggcaaatttc
     2581 atcgccagcg tcccacccat tccgtatccc accacaccgt gaatgttccg cttaatgaac
     2641 tcacgggtgt
//
LOCUS       DASTQY010000266         6164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_795459, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000266 DASTQY010000000
VERSION     DASTQY010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795459"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VFR82_08810"
     CDS             <1..1065
                     /locus_tag="VFR82_08810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4501737.1"
                     /translation="ESRKESISFYRDVIQIAVRNAWLRLYFLKVGGARIAVRIALLFQ
                     NKLYMLKAGYDPQYASYAPGHVLSMKILEEAWRLRFDEVDFLGDAERWKLDWATDVHR
                     HVWLFVFPGHLKSRLLYHLKVRVMPRIKATRRAVVRHGVRGCLTLAARHAKQNVLTSL
                     YRHERHVWYVAAPGEVHARPLPDGLKLRRSRREHLDLLTQSNLYGWSAAESYLGEGGD
                     LWMVRDSQRPVFCCWVFRKRMPTVAARGGWKDLPAKTVCFEGVVTDAEYRGRGIAPAA
                     WALVAQSLNDEGIHTIAIKVEEKNRSMRRAVEKAGFHEMAITDFRQVAGHSRMQVVPV
                     NGMSGRGADILAEVQKLAKN"
     gene            1084..2256
                     /locus_tag="VFR82_08815"
     CDS             1084..2256
                     /locus_tag="VFR82_08815"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU4501738.1"
                     /translation="MYLPAWPGLGFRQLSGMTGCHRLPFPLEAQDTTFFHTARSAIYY
                     LFKELVKSGRGVVLVPDYHMGNEVRAIRASGAQIAWYPATRRFEIDLEGLRRSCQRAR
                     AQVLFVIHYAGWPQPIEALRTFCEEYGLILVEDCAMALLTEREGRPVGTSGDYAIFCL
                     YKTLPLPDGAVLVQNRGRFDQLTALPLRRSGRIFTAGRIAELGLERIRSRWPQMGRHL
                     MDWKLAAGRLLTRLRVERVPVGDTGFDVANVDIGMASVSRRLLTRLDYEDIKKRRREH
                     FHLLAEEFTAEAVRTDLDPGVCPLFFPLLVADKGRAAQALWSRGVMATPLWNEGDVSL
                     GLHEGEASRCLRRHVLELPIHQDLGQDHLTYMARQVRNLGIALRSGSIREPEFQPV"
     gene            2365..3600
                     /locus_tag="VFR82_08820"
     CDS             2365..3600
                     /locus_tag="VFR82_08820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4501739.1"
                     /translation="MQTAAISPVEEQESVQPHERIGTGPPEEVPVKELSVDVVRDYES
                     FVALKPVWNRLVTEAGIDHPFIRHEWVRTWWECFEHHGTLHILVVREGSEPIAIAPLM
                     FDHGRLFGCPVRRLRGIANVYTERFDIILTRRAEEASRAIWKCLAADASTWDLLELRQ
                     IPNGARGVEHLRFWAFESNFLLGQWHSSDGPFIPIDRPWEAYAKTLSKKHLSNLRGRA
                     KGLNRIGEVQHEIVTGGEHLKRALDEAFLLEAAAWKGKAGTAIMNRGDRLAFYRTLLT
                     RASEAGWLQLHFLAVNGKRIAVQITLLIQNKLYILKSGYDPHFAPFAPSLLLCELMLR
                     DAWKHGLAEVDFLGNSERWKLEWAHESRPHSWVFIFPNRPPLRLLHRLKFSVLPRLQK
                     YAAYRVLRSGICGLKVDED"
     gene            3846..5231
                     /locus_tag="VFR82_08825"
     CDS             3846..5231
                     /locus_tag="VFR82_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4501740.1"
                     /translation="MVTNTLTLVNHASVLIKGKKQSILTDPWYDGEAFHRGWRLLYEN
                     KENEIEYVLNNTKFIWISHEHPDHFSIGFFRKYKSLIIDNKIVVMFQHTKDKRVISFL
                     KKGGFKSMELASGEPFALEDKFTVRVVKDEFYDSALLVNVAGINIFNLNDCAMHSQKR
                     ITAFKNQYGCCDVLLTQFSYAAWKGGRDHIEWRRSAAQDKLTSLVRQGQTLQAKTVIP
                     FASFMYFANVLNSYLNDGVNTPRRVREFCRASDADFRCVFLKPMESLDLDDNDRPKQQ
                     ETSLSFWENAFTSNKTYIQYEESYSIDQLSRLFKTYCERLKKKNAWWLIRVCRYLRIA
                     FRPITMKLVDTGEVVRVDLARRKIGPAVVDPEIALHSESLAFIFKFPYGFDTLGVNGT
                     FEELKQGGFDKFTKTFAIENLNNIGYSFKLALAFDLNVILIFIGRLMRASRKLNAGKT
                     VAREVVRARAA"
     gene            5345..>6164
                     /gene="asnB"
                     /locus_tag="VFR82_08830"
     CDS             5345..>6164
                     /gene="asnB"
                     /locus_tag="VFR82_08830"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEU4501741.1"
                     /translation="MCGIAGIMSANGRPVLEDELRAMCAAMIARGPDDEGVYLDRGIG
                     LAMRRLSIIDLQTGHQPVSNEDGSIWVVLNGEIYNFQELREELSKRGHTFSTATDTEV
                     IVHLYEEFGDRCVDRLRGMFAFALWDERRCRLLIARDRLGIKPLYYAMVQGRLLFASE
                     LKAILQLPEVDRTVNVTALDHLLAYKTTPERHSIVSAVRKLEPGHCLTLTGSGEPRVE
                     RYWSLRFEPETGRTEDDVAEELRQRLMESVRLHMVSDVPVGAFLSGGIDSSAVVA"
BASE COUNT         1323 a         1575 c         1872 g         1394 t
ORIGIN      
        1 gagagccgga aggagagtat ctctttttat cgtgatgtca tacagatcgc ggtgcgcaat
       61 gcttggcttc ggctgtattt tttaaaggtg ggcggcgctc gtatcgcggt gcgaatcgcg
      121 ctgttgttcc agaacaaact atacatgctg aaagctggat acgatccgca atacgcctct
      181 tatgctccgg gacatgtttt gtccatgaag atcctcgaag aagcctggcg attacgcttc
      241 gacgaagtgg actttctggg cgacgcagaa cgatggaagc tcgattgggc aaccgatgtg
      301 caccggcatg tctggctttt tgtgttcccc ggccatctca aatcccggct cttataccat
      361 cttaaggtcc gtgtgatgcc gcgcatcaaa gctacaagga gggcagtggt acgacacggc
      421 gtccgtggct gcctcacact cgccgcacgt catgcgaaac aaaatgtact cacgagcttg
      481 tatcggcatg agcggcatgt ctggtatgtg gctgcgccgg gcgaagttca cgcgcgtccg
      541 ttgccggacg gcttgaagct tcgccggtcc cgtcgtgaac atctcgacct tctgactcag
      601 tccaatctgt atggctggag cgcagcggaa tcgtatctgg gagaaggcgg agatctttgg
      661 atggtccggg acagccagcg gccggtgttt tgttgctggg tctttcgcaa acgcatgccg
      721 acggtcgcag cgcgaggcgg ctggaaggac ctgcccgcga agaccgtctg ttttgaaggc
      781 gtggtgacgg atgccgaata tcgcggacgc ggaatcgcgc cggcggcctg ggccttggtc
      841 gctcaaagtc tgaatgatga agggatacat accatcgcca ttaaagtgga agagaagaac
      901 cggtcaatgc gacgagcggt cgaaaaggcc gggtttcacg aaatggcgat taccgacttc
      961 cgtcaggtgg cgggacactc ccgcatgcag gtggtgcctg tcaatgggat gtccggtcgc
     1021 ggggcggaca tcctcgccga agttcaaaag cttgcaaaga attgatgggg gaggcgcaga
     1081 gcaatgtatc tgcccgcatg gccaggcctg ggatttaggc agttgtccgg catgactggc
     1141 tgccatcgtc tgccgtttcc tctcgaagca caagacacca ccttttttca cacggcccgg
     1201 agcgccatct attacctgtt taaggaactg gtgaagagcg gtcgtggcgt agtgctggtg
     1261 cccgactacc acatgggcaa cgaagtgcgg gcgattcgtg cgtccggagc gcagatcgcc
     1321 tggtatccgg ctacaaggcg gttcgagatt gacttggagg ggctgcgccg gtcctgtcag
     1381 cgcgcgcgtg cccaagtgct gtttgtcata cactatgccg gttggccgca accgatcgaa
     1441 gccctgagaa ctttctgcga agagtacggg ttgatattgg tggaggattg cgcgatggct
     1501 ctgctcacgg agcgtgaggg ccggccggtc ggcacctcgg gcgactatgc gatcttctgt
     1561 ctctataaga cgcttccgct tcccgatgga gccgtactcg tccaaaatcg agggcgtttc
     1621 gaccagttga ccgctctgcc attgcggcga agcggcagaa tcttcaccgc cggccgcatc
     1681 gctgaactcg gtttggaacg gattcgcagc cgctggccgc aaatggggcg gcacttgatg
     1741 gattggaaac tagctgccgg ccgcttgctg acacggctcc gtgttgaacg ggttccggtc
     1801 ggggataccg gattcgatgt cgcgaatgtc gatatcggga tggcgtctgt gtcgcggcga
     1861 ttgctgactc ggttggacta tgaggatatc aagaaacggc ggcgcgaaca ttttcatctc
     1921 ttggctgagg agttcactgc agaagcggtt cgcacggatc tggatccagg agtctgcccg
     1981 ttgttctttc cattgctcgt ggcagacaag gggagagcgg cacaggcact ctggagccgt
     2041 ggcgtgatgg ccacgccgct ctggaatgag ggtgatgtgt ccctcggatt gcatgaaggg
     2101 gaagcgtcac gctgtcttcg ccgtcatgtg ttggaactgc cgattcatca ggacctgggt
     2161 caggaccacc tcacatacat ggcacgacag gtaaggaatc tgggcatcgc gcttcgttcc
     2221 gggtccattc gcgagccgga attccagccg gtgtgatcca tgcgatggcg ctgaacactt
     2281 attatggatc cacgcagaaa acggatgagc cattctatgc cagcatcttc gggtcgtacc
     2341 cgggggaaaa aggagggaag tggcatgcaa acggcggcga taagtccagt ggaggagcag
     2401 gagtcggtgc agccacatga acgaatcggg accggcccgc cggaggaggt gccggtcaaa
     2461 gagttatcgg tcgatgtggt tcgggattac gaatcctttg tcgccctgaa accggtttgg
     2521 aatcgactcg tcactgaggc cggcatcgat catcctttca tccggcatga gtgggttcga
     2581 acctggtggg agtgtttcga acaccacggc accttgcaca tactggtcgt ccgggaaggg
     2641 agtgagccga tagccatcgc gccgctgatg ttcgaccatg gccgcctatt tggttgtccg
     2701 gtccgccggt tacggggcat cgccaacgtc tatacggagc ggtttgacat cattctcacc
     2761 cgtcgggcgg aagaggcctc cagagccatt tggaagtgtc ttgctgccga tgcctccaca
     2821 tgggacctgt tggaactgcg gcaaatcccg aacggcgcgc ggggcgtgga acatctccgc
     2881 ttctgggcct tcgaaagcaa ttttctgcta ggccaatggc attcatcaga cggtccattc
     2941 attccgatcg atcggccatg ggaagcctat gcgaaaaccc tgtccaagaa acatttatcc
     3001 aacctgcgcg gtcgcgcaaa gggattgaat cgaatcggag aagtccagca cgagattgtg
     3061 acaggcggtg aacacttgaa acgcgcgttg gacgaagcct tcctgttgga ggcggccgct
     3121 tggaagggga aagccggtac cgcgatcatg aatcggggcg atcgccttgc gttttaccgt
     3181 acactcttga cgcgggcgtc ggaggcagga tggctgcaac tgcatttttt ggcggtcaat
     3241 gggaagcgga tcgcggtgca aattactctg ctcattcaga acaagctcta tatcctcaaa
     3301 tcaggctatg atcctcactt tgcgcctttt gcgccctctt tgctgctgtg tgaattgatg
     3361 ctgcgcgatg cctggaaaca cggcttggcc gaggtggatt ttctcggcaa ttccgaacgc
     3421 tggaagctcg agtgggccca cgagagcagg ccgcattcct gggtctttat atttccgaat
     3481 cgcccgccgc tgcgtctatt gcatcgactg aaattctcgg tcttgccgcg gctgcagaaa
     3541 tatgccgcct atcgcgtcct ccgaagcggg atttgtggac tgaaagtcga cgaggattag
     3601 actacttcga gctcgcggca tagggtctct cagcctcttt ccggtccaac tcagtctgtg
     3661 tttgtgcttg ggcgtggtca tccctcgagc aacaggacgg cgctgacgaa cagacagatc
     3721 gtctcgcatg gccgaggcac tgccgacacc agaggcacag ggccggagag agcaggttta
     3781 cgatacgacg tgtaatgaaa gacaccggtc aaagccggtg gtgtccttga tcgttggaga
     3841 agattatggt cacgaacacg ctgacgttag tcaaccatgc atcggtactg atcaaaggta
     3901 aaaagcaatc catattgacg gatccctggt acgacgggga ggcttttcat cggggatggc
     3961 gcttgctcta tgaaaacaaa gagaacgaga ttgagtatgt gctgaataac accaagttta
     4021 tttggatttc gcatgagcat ccagaccact tttccatagg cttttttaga aagtacaaga
     4081 gtttgatcat tgacaacaag atcgtcgtga tgtttcagca cacgaaagat aagcgtgtca
     4141 tcagctttct caaaaaaggc ggcttcaaaa gtatggaact tgcttctgga gagccgttcg
     4201 ctctggaaga caagttcacc gttcgcgtgg tcaaagacga attttacgac tccgcactac
     4261 ttgtcaatgt ggcaggaatt aacattttca atctgaacga ttgtgccatg cattcccaga
     4321 aacgcattac ggcttttaag aaccaatatg gttgttgtga tgtattgctc acccagttca
     4381 gttacgcggc gtggaagggg gggcgggacc atattgagtg gcggcggtca gcggcgcagg
     4441 ataaattgac gagcctagtc agacaagggc aaacgttgca ggccaagacc gtcattccct
     4501 tcgcaagctt tatgtacttt gccaacgtgt tgaacagcta cctgaatgat ggcgtgaaca
     4561 cgcctcgacg cgtccgggaa ttctgtcgcg cttcggacgc cgactttcgg tgtgtctttc
     4621 tcaaaccgat ggagtccctc gatttggacg acaacgaccg tccgaagcaa caggagacgt
     4681 ccctgtcgtt ttgggagaat gcgttcacat ccaataaaac atacattcaa tatgaggaat
     4741 cctactcgat tgatcaactg tctagattgt tcaagaccta ctgcgagagg ctgaaaaaga
     4801 agaatgcctg gtggttgatt cgggtgtgcc ggtatctgcg gatcgcattc aggccaatca
     4861 cgatgaagct tgtggatacg ggagaagtcg tccgtgtgga tttagcgaga cgcaagattg
     4921 gtccggccgt agttgacccg gaaattgcgc tgcattctga atcgttggcg ttcattttca
     4981 agtttcctta cgggttcgat acgcttgggg tgaacgggac atttgaagaa ctgaaacagg
     5041 gtgggttcga taagttcacc aagacctttg cgatcgaaaa cctgaacaac atcgggtatt
     5101 ccttcaaatt agcactcgca ttcgatctga atgtgatact gattttcatc ggaaggctca
     5161 tgcgggcatc gcgcaagctg aacgcgggta agacagtggc ccgtgaagtc gtcagagcca
     5221 gagcagcctg acttgacctg cgttcgcgat cggaccaatt cctcctcatc gtgtctcatc
     5281 ggtgagggag ggctcatcct gcttgagccc ttccaattca caacttgcgg aaggacgatt
     5341 cggtatgtgc ggtattgccg gcatcatgag tgcgaacggt cgtccggttc tcgaggacga
     5401 gttgcgggcc atgtgcgcgg cgatgattgc gcgtggacca gacgacgagg gggtgtatct
     5461 cgaccgagga atcggtctcg caatgcgccg cctgagcatt atcgacttgc aaacgggaca
     5521 tcagcccgta tccaacgagg atggctcgat ctgggtggtg ttgaacgggg aaatctataa
     5581 ctttcaggaa ctgcgggagg aattgagcaa gcgcggccat acgttttcga ccgcaactga
     5641 caccgaagtc atcgtgcatc tgtacgaaga gttcggagat cgatgcgttg accgtctccg
     5701 tgggatgttc gccttcgcgc tctgggacga acgccgatgc cggctgctga ttgcccgcga
     5761 ccggctgggc atcaagccgt tgtattacgc aatggttcaa ggccggttgc tgttcgcgtc
     5821 tgaactgaaa gccatcttgc agctgcccga ggtggaccgg accgtgaatg tcaccgcgct
     5881 cgatcatctg ctggcgtaca aaacgacgcc ggagcggcac agcatcgtgt cagccgttcg
     5941 gaagctggag cccggccatt gcctcaccct cactgggtca ggcgaaccac gtgtcgagcg
     6001 ctattggtcg ttgcgctttg aaccggagac gggacggacg gaagacgacg tcgcagagga
     6061 attgcggcag cgtctcatgg aatcggtcag gctgcatatg gtcagcgatg tccccgtggg
     6121 cgcgtttctg agcggcggca tcgattccag cgcggtcgtc gcgg
//
LOCUS       DASTQY010000267        12526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1162635, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000267 DASTQY010000000
VERSION     DASTQY010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 12526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12526
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162635"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFR82_08835"
     CDS             <1..257
                     /locus_tag="VFR82_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501742.1"
                     /translation="AFLDQLRPGDLFIITGDHGRDPNKPHAVPTREWVPLLITGPKLA
                     QGVNFGLRPTASDLGQTVVEALKGESLTVGESFLDVLRPG"
     gene            315..773
                     /locus_tag="VFR82_08840"
     CDS             315..773
                     /locus_tag="VFR82_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU4501743.1"
                     /translation="MTYEEKLKSLGLELPEAPKPVATYVPAVRTGNLLFLSGVLPMRN
                     GQLAFTGKLGRELTVLEGIEAAKVAILNALAIAKQELGSLDRITRVVKVVGHVASADG
                     FTEQPQVLNGASDLLVAIFGDAGRHARVATGAAELPRRAAVEIEVILAVS"
     gene            931..2628
                     /locus_tag="VFR82_08845"
     CDS             931..2628
                     /locus_tag="VFR82_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEU4501744.1"
                     /translation="MMAKTYLSRWIIVFGTVLLAALGSYQGTEAAPSSPLELQPATGV
                     PGATVSIVGKGLGRFKSAQVNRVLFGGVPALIQRWESDVVEVKVPFKAQTGPVQVLIG
                     KKKLAAGTFSVVYPKIVSIHPAAIEPGHAVEIVGEHFGITAGPRDPNTMFGVNDVIVG
                     NLVVRPRRWKDDKLEVDVPANATSGDIAVRLASSDPLPDGSCCAPVQHTVSNAVSLKL
                     IPTVQVDPLTGPVGTKVVLFGQGFGQERTAGDTITIGGHEAPVAQWSDRAIVVHVPVN
                     AETGPIVLSRRGQDRTLGTFTVQIPKATGLSPASAPIGTLVRIQGEHFGAYSESGATP
                     YNYTDFDTGQNRVEIGGAPAVIYRWHDDRIDIWVPFSAKSGPVVVTRGAPKPAADGSC
                     CRERGTVSTTAGAFTLVVPTIESFTPASAGLDEVVTIKGSGFGSFIKTTEATQPGLGE
                     GAFKRNQIELGENVSRTEVLFNNIGAIVVSWTDTEIKARVPHRHLFGIGKPGQFNADL
                     STGPLIIRRGSWDLLPDGTCCTPKKWLTLEAGTFTIEAKGMPDQSFFQSRPEGSTND"
     gene            2741..3703
                     /locus_tag="VFR82_08850"
     CDS             2741..3703
                     /locus_tag="VFR82_08850"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEU4501745.1"
                     /translation="MTILLFVPLSLLLSTFIISPTRAAESQLKAAPQKTGTEVTLMSV
                     YFRDANLGWAVGSGGTVVKTVDGGQKWKKQASGTSVQLTGVFFVDDKQGWIIGANGTI
                     RHTMDGGANWRAQTIDTVAPMYGLSFVSPSEGWVVGGNGTILHTTDGGAHWTDQSSPT
                     TAALYAVQFLTPKRGTAVGAVGTVLATEDGGTSWLAQETQGSVTLFDVYFSDPLTGWV
                     VGNAGAMFQTTDGGGRWVDRTLPCSRTCTRLTDLLRIRFTDPKTGWIVGERGMAYHTA
                     DSGFTWEEQGAVANVSLFGLSFPTTLHGWASGEKGTVVHLKLAK"
     gene            complement(3706..4449)
                     /locus_tag="VFR82_08855"
     CDS             complement(3706..4449)
                     /locus_tag="VFR82_08855"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU4501746.1"
                     /translation="MPTFFVATDSVVPPTVRITGPLLHHLRQSLRVQPGEPLTVTDDS
                     GRRYRTEVGEITARHLTARILETTTAPPKTSPSIVLAQALLKGEKMDWIIQKATELGV
                     DRIAPVVTSHGVVKPRAERLEHQRARWQRIALEAAQQCERWSVPTIDEPATVSQLMTA
                     YDTAGAIKLFLAERSSGASLADIAAGLGAEQTVLLVVGPEGGWDKEELRLAQERGCQA
                     ITMGKNILRAETAALAAISVLQARFGELG"
     gene            complement(4480..5607)
                     /gene="dnaJ"
                     /locus_tag="VFR82_08860"
     CDS             complement(4480..5607)
                     /gene="dnaJ"
                     /locus_tag="VFR82_08860"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU4501747.1"
                     /translation="MASVSKRDFYETLGVERTASEDDLKKAYRKLARQYHPDLQTGEQ
                     QKKHAEEKFKEINEAYEHLSDQEKRKRYDMFGHAGTQAGSGFEGFDFGRGGGFGDVFN
                     DIFEDFFGGQRGGNRAERGNDLQYNLEITFEESVYGKEAKLKIPRWETCSDCKGSGAK
                     SASSVKTCPSCKGAGQIRLQQGFFSVSRPCGQCEGSGHIITEPCTTCQGRQRTYRERT
                     ISVHIPAGIETGMRLRLSNEGEHGINGGPAGDLYVAITVKAHPIFQRKGNDIVCDVPI
                     NFVTAALGGKIEVPTLKGTTVMKVPAGTQPDKVLRLKGLGIPSLKNQTTGDQLFTIKV
                     HIPTKLTAKQRELLTEFAKESGMSMEADGDGFFDKMKTFFE"
     gene            complement(5686..7614)
                     /gene="dnaK"
                     /locus_tag="VFR82_08865"
     CDS             complement(5686..7614)
                     /gene="dnaK"
                     /locus_tag="VFR82_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU4501748.1"
                     /translation="MGKVIGIDLGTTNSCVAVMSGGDPVVIANAEGARTTPSVVAITD
                     KGERLVGQIAKRQAITNPENTIFSVKRLMGRKFRSKEVQEAIKRLPYKVVEADNGDAH
                     VELRGKRYSPPEISAMILQKMRQTAEDYLGEKVTEAVVTVPAYFDDSQRQATKDAGQI
                     AGLNVLRIINEPTAASLSYGLDKKKDERISVYDLGGGTFDVSVLEIGEGVFEVKSTNG
                     DTYLGGDDFDLRVMDWLVDEFRKDQGIDLRKDRMALQRLKEAAERAKIELSSSQETEI
                     NLPFITADASGPKHMVIKLTRARLEQLVDDLIQRTVEPCRKALADASVSARDINEVVL
                     VGGMTRMPKVIQVVKDFFGKEPHRGVNPDEVVAIGAAIQGGVLKGDVKDVLLLDVTPL
                     SLGIETLGGVFTKLIERNTTVPTKKSQVFSTAADNQTAVTIRVFQGEREMANDNKLLG
                     QFDLVGIPSAPRGMPQIEVAFDIDANGIVHVSAKDLATQKEQSIKITASSGLSKDEVE
                     KLVRDAQTHTEEDKKRRKAAEAKNQADNLLYQTEKNLKEHGDKISGDDRTKIEEAVTA
                     LKKAMEGTDPDAIESATQALTTASHKLAEEMYKKASASAATGAGTETGGGAESPNGAK
                     TDEKVVDAEFEEVDKDKK"
     gene            complement(7650..8225)
                     /gene="grpE"
                     /locus_tag="VFR82_08870"
     CDS             complement(7650..8225)
                     /gene="grpE"
                     /locus_tag="VFR82_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU4501749.1"
                     /translation="MADESLNTNTFDDLDSDSGGAASSGDSLADSAAKDGELKTLNDK
                     YLRLAAEFDNYKRLAQRDQRDQIRFGNEQLLKELLPVVDNLERAIKAAKDSKTAQGLV
                     QGVDLTLKQLEGVLGKFGVQSIPTTGQRFDPSGHQAVASVPSTQVPDQHVVEEFQRGY
                     RLHDRILRPAMVTVSSGGNSASNGASDESDS"
     gene            complement(8315..9103)
                     /locus_tag="VFR82_08875"
     CDS             complement(8315..9103)
                     /locus_tag="VFR82_08875"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEU4501750.1"
                     /translation="MLLVADIGNTNVVLGIYDGQVLKAHWRLATDAKTTADEYGILFT
                     NLLASAGILPRQVSGAIMSSVVPTLTATFETLIEQYFHQRPLIVTSDTDTGLTIRYPN
                     PKEIGSDRLVNAAAAYHKYRRDMIIVDFGTATTFCAVTKDAEYLGGVIAPGLGISAEA
                     LFTRAAKLSKVELARPKTVIGTDTGSSIQSGLLFGYAGLVDTLVKRIEQELGRSTYVI
                     ATGGLSSIIASEATSIQIIEPLLTLDGLALLYRRANGSAPTAWV"
     gene            complement(9149..9982)
                     /locus_tag="VFR82_08880"
     CDS             complement(9149..9982)
                     /locus_tag="VFR82_08880"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEU4501751.1"
                     /translation="MKHTSPLTLGGIESTLATRSLGRRIELRDRIDSTNREAMVLGQA
                     DAEHGTLVLADSQTAGRGRLGRVWFSPPGINLYASIVIRRTLDAQRLSQWLSWLPLMT
                     ALATAEAIEAAGTARVSLKWPNDLLIGNRKVGGILCESGASIRSGPFQVIGIGINVNG
                     ERASFPRGLRDVATSIRQETGQEVDRHRLLVRLLEELEVCLEEFLGHGIERLALAYRR
                     RCTTLGKTVKAVLADGNEYVGTADGIGDDGSLTVLQEPARPGVPFEVRHLRAADITHL
                     R"
     gene            complement(9986..10861)
                     /gene="nadC"
                     /locus_tag="VFR82_08885"
     CDS             complement(9986..10861)
                     /gene="nadC"
                     /locus_tag="VFR82_08885"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU4501752.1"
                     /translation="MPELPAADIRRAVRLALEEDLGQGDVTTSALFPSAVPARARIVA
                     QQPLVVAGMAAAVQTFLTVDASLTLAVLKKDGAQALNRETILRIEGDGRSILMAERVA
                     LNFLQQLSGIATLTQRFRQAVDGRVAILDTRKTIPGWRSIQKWAVALGGGTNHRRSLA
                     DGILVKDNHLKLLRHKRAVLEACKLARHRGPSGRQVIVEAESLKEVAQAVAGQADIIL
                     LDNMTPAMVKTAIKLIDGRALVEVSGGITLDNVRAMAAAKPDRISIGALTHSAPAATL
                     TLELERAVQGRQKKR"
     gene            complement(10864..>12526)
                     /locus_tag="VFR82_08890"
     CDS             complement(10864..>12526)
                     /locus_tag="VFR82_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU4501753.1"
                     /translation="YLSPQWFVEIKPLAEPAIRAVEEGRIRIIPEGWINNYLGWMRDI
                     KDWCISRQIWWGHQIPAWYCEHCNERFIHRQTSTNQAGDVVTRITLLQGATPVVARAT
                     PAKCPQCGGEQWLRDPDVLDTWFSSGLWPFSTLGWPEQTPELKTYYPTSTLVTGLDIL
                     FFWVARMIMLGLRFMNDVPFRDVYIHALVRDAEGQKMSKSKGNVIDPLHVMDQFGTDA
                     LRFTLASMASPGRDLKLAEERIEGYRNFANKIWNAARFGLMYLDGARTAARPSERPFS
                     DRWILSRLNHAIITVTSELEAYRFDRAASALYHFIWHEFCDWYVELIKPVLQNPSHAD
                     GPVTRQTLVETLEVTMRLLHPFMPFITEEIWQTIPHEGESIVIRPYPTSDQTWDDAEA
                     EEQFLLLEQTVSLVRTGRVLLNYPPGQPIEFSMTHDDPAKLRQLEHLQPHVAHLSRGT
                     ADMLSKPVSPAAQRLRLVTGGLSIALIVSGDVDLKKALDRIVKQREQQEKDMMRLEGK
                     LNNHEFRGKAPADVLAEHESRLRSLQNELALLASSERQLRALLGP"
BASE COUNT         2386 a         3677 c         3686 g         2777 t
ORIGIN      
        1 cggcattctt ggatcaattg cgccccggcg acctatttat catcaccggc gaccatggac
       61 gggatccaaa caagcctcat gcggttccga cccgggagtg ggtacctctc ctcattaccg
      121 gccccaaact ggcccagggc gtaaacttcg gcttgagacc cacggcctct gacctcggtc
      181 aaaccgtcgt cgaagcgctg aaaggggaat ccttgaccgt cggagagagt tttctcgacg
      241 tgctgaggcc gggatgacca gcatgacacc tgtgatctga aaatccggag cattgagcgg
      301 agtggacttt ctgaatgacg tatgaagaaa agttgaaatc cctgggatta gaactgccgg
      361 aggcgccgaa gcctgtggcg acctatgtcc ctgcggtgcg gaccggcaat ctgttattcc
      421 tgtcgggcgt cctgccgatg cgaaacggac aattggcatt caccggcaaa ctcggacgtg
      481 aattgaccgt tctcgaaggg atcgaggcag ccaaggtcgc gatcctcaat gcgctagcca
      541 ttgcgaaaca ggaactggga agtctcgacc gtatcacgcg cgtcgtgaaa gtggtcggtc
      601 atgtcgcctc ggcagacgga tttaccgagc agccgcaggt gttaaacggc gcctccgatc
      661 tgctcgtcgc gatctttgga gacgcgggac gccatgcgcg cgtcgccact ggagccgccg
      721 aacttccgcg tcgcgccgcc gtggaaatcg aagtcatcct ggcagtttcc tgaataaaat
      781 gaaggcccta gaggcaggtt ccgcgtcctc tgcggcttga cacccgaaaa aaccgcttgt
      841 tatagtccgt cgtccctcgc ttcacgctgt accccggtta ccctccagac tcgtgcgtgg
      901 ggttctcgtg ccacgcaggg ctctcaacac atgatggcca agacctatct cagtcgctgg
      961 atcattgtct tcggtactgt cttgctggcg gccttgggca gttaccaggg aacggaggct
     1021 gcgccctctt cccctctcga actccagcct gcgaccggcg tgcccggcgc gacggtatcc
     1081 atcgtcggca aggggttggg tcgcttcaag tctgctcaag tgaaccgcgt gttgttcgga
     1141 ggagtgccag cgctgatcca acgctgggag tcagacgtcg tggaagtgaa agtgcccttc
     1201 aaagcacaga ccggtccggt tcaggtgctg atcggcaaga agaaactcgc ggccggcacc
     1261 ttttcggtcg tctatccgaa aattgtttcc attcaccctg ccgccatcga acctggccat
     1321 gcggtcgaaa ttgtgggcga gcattttggt atcacagccg gaccccggga tcccaatacg
     1381 atgttcggcg tcaacgacgt gatcgtgggg aacttggtgg tcaggccgag acgctggaag
     1441 gacgacaaac tcgaagtgga cgtgccggcc aatgcgacgt ctggagacat cgcggttcgc
     1501 ttggcttctt ccgatcctct gccagacgga tcctgttgcg cgccggttca acataccgtc
     1561 agcaacgctg tatcgctgaa actgattccg actgtgcagg tcgatccgct gaccggcccc
     1621 gtgggcacga aagtcgtgct gttcggtcag ggatttggtc aagaaaggac ggccggcgat
     1681 accattacga tcgggggaca tgaggccccc gttgctcagt ggagcgaccg cgccatcgtc
     1741 gtgcatgtgc ctgtcaacgc ggaaacagga cccatcgtct tatcccgtcg gggacaagat
     1801 cggacactcg gaacctttac cgtccagatc cctaaagcca ccggtctctc tcctgcgagc
     1861 gcccccatcg gcacgctggt cagaatccaa ggagagcact ttggcgcgta ttcagagagc
     1921 ggcgcgacgc cctacaacta tacagacttc gataccggtc aaaatcgcgt ggaaatcggc
     1981 ggtgcaccgg cggtgattta ccgttggcat gacgatcgga tcgacatctg ggtgccattc
     2041 agcgccaaaa gcggtccagt cgtcgtcaca cgcggtgcgc ccaagccggc ggcggatgga
     2101 tcctgctgtc gtgaacgagg gacggtatcg acaaccgccg gagcattcac gctcgttgtg
     2161 ccgaccatcg agtccttcac gcctgcttca gccgggttgg acgaagtggt gaccatcaaa
     2221 gggtctggat tcggctcgtt tatcaagacg acagaagcca ctcagccggg acttggagag
     2281 ggcgccttca agcgcaatca gatcgaactc ggcgaaaacg tctcgcgaac cgaggtcttg
     2341 ttcaataaca tcggcgccat tgtggtgtcc tggaccgata cggaaatcaa ggcgcgcgtg
     2401 ccgcatcgtc atttgttcgg gatcggcaag ccgggacagt tcaacgccga tctctcgacc
     2461 ggtcctctaa tcattcggcg gggctcctgg gacctgcttc cggacggcac ctgctgtacc
     2521 cccaaaaagt ggctgacgct cgaagccggc acattcacga tcgaagccaa gggcatgccc
     2581 gaccaaagct ttttccagtc caggcccgaa ggcagcacca acgactagca ggatggtcca
     2641 aaatcccgcc agcttcgttc tcgcatcgct tagaggctca acgtacaact caaagtacgc
     2701 ttcgcctctg cgctcgctgc ggccttgctg gacgagattt ttgaccatcc tgcttttcgt
     2761 ccccctcagt ctgcttttaa gcacattcat tatttcaccc acaagagctg ccgagtcgca
     2821 gctgaaagcc gctcctcaaa aaacggggac ggaagtgacg ctcatgagcg tctattttcg
     2881 cgatgccaac ctgggatggg cggtcggctc cgggggaacg gtcgtcaaga ccgtggacgg
     2941 ggggcaaaaa tggaagaaac aggccagcgg cacctcggta caactgacgg gagtattttt
     3001 tgtcgacgac aagcagggat ggatcatcgg cgctaatggg acgattcgcc atacaatgga
     3061 cggaggtgcc aattggcggg cgcagacgat cgatactgtg gcgcccatgt atggtttgtc
     3121 ctttgtttcg ccatccgagg ggtgggtcgt aggaggcaac ggaacgattc ttcatacgac
     3181 cgatggagga gcccactgga cggatcagtc cagccccacc actgctgccc tttatgccgt
     3241 gcaattcctg acgccgaagc ggggaactgc cgtgggcgct gttgggaccg tcctggccac
     3301 ggaagacggc ggcacttcat ggctggccca ggaaacccag ggatccgtca cgctgttcga
     3361 tgtctatttt tccgatccgt tgaccggctg ggtggttggc aatgcaggcg ccatgtttca
     3421 gacgaccgac ggagggggcc gctgggtcga tcggaccttg ccttgttcac ggacctgtac
     3481 caggctgacg gacttgcttc gcattcggtt caccgacccc aaaaccggct ggatcgtggg
     3541 cgaacgcggg atggcttatc acactgcgga cagcggattt acctgggaag aacagggagc
     3601 ggtcgcgaat gtctcgctct tcggcttgtc ttttcccacg actctgcacg gctgggccag
     3661 cggagaaaag ggcaccgtcg tgcatctcaa attggccaag taagcctatc ccaattcgcc
     3721 gaacctcgcc tgtagcacgc taatggcggc gagtgccgcc gtttccgctc gaaggatatt
     3781 tttccccatc gtgatcgcct gacagccccg ttcttgagcg aggcgcaatt cttctttgtc
     3841 ccatccacct tccggtccaa cgacgagtag gaccgtttgc tctgcaccca ggccggctgc
     3901 aatgtcggcc aacgaggctc cgctcgatcg ttcggctaga aacagcttga tggcaccggc
     3961 ggtgtcatag gcggtcatta actgggagac tgtagccggt tcatcgatcg tcggcacaga
     4021 ccaccgttca cactgctgcg cggcttcgag ggcgatgcgc tgccatcgcg ctcgttgatg
     4081 ttcgaggcgt tcggcgcgag gcttgacgac gccgtgagac gtcaccaccg gcgcgatccg
     4141 atcgacaccc agttccgtgg ccttctggat gatccaatcc atcttttccc ctttgaggag
     4201 ggcttgagcc aggacgatcg acggactggt tttcggcgga gcggttgttg tttcaagaat
     4261 gcgcgcagtg aggtgacggg ccgtgatctc acccacctcc gtgcggtagc gtcgcccaga
     4321 gtcgtcggta accgtgagcg gttcacctgg ctgcacgcgg aggctctgcc ggagatgatg
     4381 gagcagcggg cccgtgatac gcacggtcgg cgggacgacc gaatcggttg ccacgaagaa
     4441 cgtcggcatg cggaaacgga atctcgagtg ggttgcgcgt tactcgaaaa aggtcttcat
     4501 cttgtcgaag aagccgtcgc cgtcggcctc catagacatg ccgctctctt tggcgaattc
     4561 ggtcagcagt tctcgctgct tggcagtcag tttggtagga atatggacct tgatcgtgaa
     4621 caattgatcg ccggtcgtct ggttcttgag actgggaatg ccgagtccct tcagccgtaa
     4681 cactttatcc ggctgcgtgc ctgcgggcac cttcatcacg gtcgtgccct tgagggtcgg
     4741 cacttcaatc tttcctccga gcgccgccgt gacaaagttg atcggcacgt cacagacgat
     4801 gtcgttgcct ttgcgctgga aaataggatg cgctttgacg gtaatagcga cgtataaatc
     4861 tcccgccggc cccccgttga tgccatgttc gccttcgttc gacagacgca agcgcatgcc
     4921 ggtttcaatg ccggccggaa tgtggacgga aatcgtgcgc tcgcgatagg tccgttgacg
     4981 gccctggcag gtcgtgcagg gttccgtaat gatatgtccg gaaccttcac attggccgca
     5041 aggccggctg acgctgaaga aaccctgttg aagccggatc tggcctgcgc ccttacaact
     5101 ggggcaggtc ttcaccgacg aggccgattt ggcaccgctg cctttgcaat cgctgcacgt
     5161 ctcccagcgg gggatcttga gtttggcttc tttgccgtag actgactctt caaacgtgat
     5221 ttcgagattg tactgaagat cgttgccgcg ctctgcacgg ttgccgccgc gctggccgcc
     5281 gaaaaaatct tcaaagatgt cgttgaaaac atccccgaag ccgccgcccc ggccaaaatc
     5341 aaagccttca aagcccgagc ccgcctgggt gccggcatgg ccgaacatat cgtagcgctt
     5401 gcgcttttcc tgatcgctca ggtgttcata ggcttcgttg atctctttga acttttcctc
     5461 ggcatgcttc ttctgctgct cgccggtctg caggtcggga tgatattggc gggcgagttt
     5521 ccgataggct ttcttgaggt catcctcgga cgccgtccgt tcgacgccca gtgtttcgta
     5581 gaaatctcgt tttgacacgg aagccattgt ccctttcagt ggttgcgcag aaagaccgag
     5641 tcccgtgatc gacgaggctc ggtccctgag tagccgtatt ctagcttact ttttatcttt
     5701 gtctacttct tcaaactctg cgtccaccac tttttcatcg gtctttgcgc cgttcggact
     5761 ctcggccccg ccgccggtct ctgtgccggc accggtcgct gcggaggccg acgccttctt
     5821 atacatctcc tcagccagct tatgcgaggc agtcgtcaac gcctgcgtgg ccgactcgat
     5881 cgcatcggga tcggtgcctt ccatggcttt cttcagcgca gtaaccgctt cctcgatctt
     5941 ggtcctgtca tcgccggaga tcttgtcgcc gtgctccttg agattcttct ccgtttgata
     6001 gagcaggttg tccgcctgat ttttcgcttc cgccgctttc cggcgtttct tgtcctcttc
     6061 ggtatgcgtc tgcgcatccc tgacgagttt ttccacttcg tccttgctca ggccgctgga
     6121 tgcggtgatc ttgatggact gttctttttg tgtcgccagg tccttggccg acacgtgcac
     6181 aatcccgttc gcatcgatat cgaacgccac ttcgatctgg ggcatgccgc gcggggcgga
     6241 gggaatgccg acgagatcga attggccaag caatttattg tcgttggcca tctcacgttc
     6301 tccttggaac acgcggatgg tcaccgctgt ctggttgtcg gccgccgtcg aaaacacctg
     6361 actcttcttc gtgggaacgg tcgtattgcg ctcgatgagc ttggtgaaga cgccgcccaa
     6421 cgtttcgatg ccgagtgaca acggcgtgac gtcgagcaag agcacgtcct tgacgtcgcc
     6481 tttgaggacg ccgccctgga tggctgctcc gatggccacc acttcgtctg gattcacgcc
     6541 gcgatgcggc tctttcccga aaaagtcttt caccacttga atgactttcg gcatgcgggt
     6601 catcccgccg acgagcacga cttcgttgat gtcgcgggcc gacacgctgg cgtcagcgag
     6661 ggctttccgg cagggttcca cggtccgctg gatcaagtcg tccaccagtt gttccagccg
     6721 cgcgcgggtc agcttgatca ccatgtgttt gggaccgctg gcatcggcgg ttataaatgg
     6781 gagattgatt tccgtttctt gtgaggatga cagctcgatc ttggcacgtt cggccgcttc
     6841 tttcagccgt tgcagcgcca tgcgatcctt gcgtagatcg attccctgat cctttctaaa
     6901 ctcgtcgacg agccaatcca tgacgcgcag gtcgaaatcg tcgccgccaa ggtaggtgtc
     6961 cccgttcgtc gatttgacct caaacacccc ttcgccgatt tccaggaccg agacgtcgaa
     7021 cgttccgccg cccaggtcat agactgaaat ccgctcgtct ttctttttat cgagaccata
     7081 ggacaatgac gccgcagtgg gctcgttgat gatgcgcagg acgttcaatc cggcgatctg
     7141 accggcgtcc ttggtggctt gccgctggct gtcgtcgaag tatgccggga cggtcacgac
     7201 ggcttccgtg actttctcgc ccaggtagtc ctctgccgtc tgcctcattt tttgcaggat
     7261 catggcagag atttccggcg gactgtagcg tttaccacgc agttccacat gggcatcgcc
     7321 gttgtcggct tcgacgacct tgtacggcag ccgcttgatg gcttcctgca cttccttgct
     7381 gcggaacttg cgccccatca ggcgtttgac ggagaaaatg gtgttttcgg gattggtaat
     7441 ggcctggcgc ttggcgattt gaccgacgag ccgttcgccc ttgtcggtga tcgccacgac
     7501 ggatggcgtt gtgcgggccc cttccgcatt cgcaatcaca accggatcgc cgccgctcat
     7561 gacggcgacg catgagttgg ttgtccctaa atcgatgccg atgactttac ccatgatgtg
     7621 ctccttcctg gactaagaat cttcgatagt catgaatcgg attcgtcgct ggctccattc
     7681 gacgccgaat tcccgcctga tgacacggtc accatcgcgg gtctgaggat ccggtcatgc
     7741 agccggtatc cacgctgaaa ctcttccaca acatgctggt ccggtacctg ggtcgaaggc
     7801 accgaagcga ccgcttgatg accggacggg tcaaagcgtt gccccgtggt cgggatggac
     7861 tggactccga acttgcccag cacaccttcc agttgcttga gcgtaagatc gacgccctga
     7921 accagtccct gtgcggtctt gctgtccttc gctgccttga tggcccgttc tagattatcg
     7981 acgacaggca ggagttcctt cagcagttgc tcgttgccga accggatttg gtcccgctgg
     8041 tcgcgctggg caagccgctt atagttgtcg aactcggcgg caagccggag gtacttgtcg
     8101 ttcagcgtct tcagctcgcc gtccttcgcc gccgaatcag ccagcgagtc accggaagaa
     8161 gcagctccac cagaatcaga gtctaagtcg tcgaatgtat tggtgtttaa tgattcgtct
     8221 gccacgatca atcctgttac agacgcgaga tagtcaccac gaccaataag tcaacagcac
     8281 cgagaaaaat taattacttg tgagccacag agactcaaac ccaggcagtc ggagcggagc
     8341 cgttggcgcg gcgatagagc agtgccagcc cgtcaagagt gagcaagggt tcgatgatct
     8401 gaatcgaggt ggcctcagag gcaataatgg aacttaaccc accggtggca ataacgtagg
     8461 ttgaacggcc gagctcttgt tcgatgcgct tgaccagcgt atcgacaagg ccggcgtagc
     8521 caaagagcaa gccagactgg atgctactgc cggtgtcggt gccgataact gttttcggcc
     8581 tggccaattc aactttcgac agcttggctg cccgcgtaaa gagcgcctcg gcagaaattc
     8641 ccagccccgg cgcgatgact ccgcccaaat attcagcgtc ttttgtcact gcacagaacg
     8701 tcgtggccgt cccgaagtcc acgataatca tgtcccgccg atacttgtga taggcggcgg
     8761 ctgcattgac gagccggtcg ctgccgatct ctttggggtt cggataccgg atcgtcaacc
     8821 ctgtatccgt atccgacgtg acgatcagcg gccgttgatg aaagtattgc tcgatcagcg
     8881 tttcgaacgt ggcggtcagc gtcggtacga cgctggacat gatcgcacca gacacctgcc
     8941 gtggaagtat gccagcggaa gccagcaggt tggtgaacag aatcccgtac tcatcggcgg
     9001 tggttttcgc atctgtggcc agccgccaat gcgccttcag cacctgacca tcgtagatgc
     9061 ccaaaaccac gtttgtattt ccgatgtcgg cgaccaacag catgcggcgt attctacggc
     9121 cttggcaatg gtttcgccac cgaaagcttc atcggaggtg cgtgatatcc gcggcgcgca
     9181 gatgccggac ttcgaacgga actccgggtc gcgcgggttc ttgaagcacc gtcaacgagc
     9241 cgtcatcacc gatgccgtcg gctgtgccga catattcatt cccatccgcc agcaccgcct
     9301 tgaccgtttt cccgagtgtc gtacaccggc gccggtacgc caatgcgagt cgttcgattc
     9361 cgtgccccag gaactcctcg aggcatactt ccagttcctc cagcagccgg acgaggagtc
     9421 ggtggcggtc gacctcttgt ccggtctcct gtcggatgga cgtagcgacg tctcgaagcc
     9481 cgcgaggaaa actcgctcgt tctccattga cattgatgcc gatgccgatc acttgaaatg
     9541 gccccgaacg tatcgaagca ccgctttcac acagaatgcc cccgactttg cgattgccta
     9601 tgaggagatc gttcggccat ttgaggctga ctctggctgt gccagcggct tcaatcgcct
     9661 cagccgtagc caacgcggtc atcagcggta gccaagagag ccattgagac agacgttggg
     9721 catccagcgt tctcctgatc acgattgagg catagaggtt gatccctggt ggagaaaacc
     9781 atacacggcc gagccggcct cgtcctgccg tttgtgagtc cgcgagcacc agcgtgccat
     9841 gttcggcatc tgcctggcct agcaccatcg cttcccggtt cgtcgagtca atgcgatccc
     9901 gcaattcgat tcggcgtccc agggatcggg tcgcgagagt gctttcaata ccgccgagag
     9961 tcaagggcga ggtgtgtttc actggttagc gtttcttctg gcggccttgg actgcgcgtt
    10021 ccagttcgag cgtcagggtt gcggcaggag ccgagtgagt cagtgcgccg atcgaaatcc
    10081 ggtcgggctt cgccgcagcc atggcccgca cgttgtctag ggtaatgcct cctgagactt
    10141 cgacgagcgc gcgtccgtcg atcaatttaa tggccgtctt gaccatggcg ggcgtcatgt
    10201 tgtcgaggag aataatgtcc gcctgtcccg cgaccgcctg ggcgacctcc ttgagagatt
    10261 ccgcctccac gatcacctgt ctgccggacg ggcccctgtg tcgtgccagt ttgcaggctt
    10321 cgaggaccgc acgtttgtgt ctcaatagtt tcagatgatt gtctttgacc agaatcccat
    10381 cggcaagcga gcgtcggtga ttcgttcctc caccaagggc cacggcccat ttttgaatcg
    10441 accgccatcc ggggatcgtt ttcctcgtat cgagaatggc gacgcggccg tccacggcct
    10501 ggcgaaatcg ctgtgtgagc gtggcgatgc cggaaagctg ctgcaggaag ttcagcgcga
    10561 cccgttccgc catcaatatt gatcggccgt ctccttcaat gcgcaggatt gtctcgcggt
    10621 tcaacgcctg cgcgccgtcc ttcttgagca cggcgagtgt gagcgaggca tcaaccgtca
    10681 ggaaagtctg gacggcggca gccatgccgg ccacgacgag cggctgttgg gcgacgatgc
    10741 gtgcccgggc gggaactgca gatggaaaaa gggccgatgt ggtcacgtcg ccttggccga
    10801 ggtcttcttc gagagcgagc cggacggcgc gccgaatgtc ggcagctggc agctctggca
    10861 tggtcacggc ccgagcaggg cgcgcagttg tcgctcgctg ctggcaagca gcgccagctc
    10921 gttctgcagg ctccgcagtc ggctctcgtg ttctgcaagg acgtccgccg gagctttgcc
    10981 ccggaactca tggttattca gtttcccctc gaggcgcatc atgtcctttt cctgctgttc
    11041 ccgttgcttg acgatgcggt ccaacgcttt cttgagatcg acgtcaccag acacgatcaa
    11101 ggcgatcgaa agccctccgg taacgaggcg aaggcgctgt gcggcaggac tgaccggctt
    11161 ggaaagcatg tcagccgttc cgcggctgag atgagccaca tgcggctgaa ggtgttcgag
    11221 ttgccgcagc ttggcgggat cgtcatgcgt cattgaaaac tcgatcggct ggcccggcgg
    11281 atagttcagc aacactcgcc cagtccggac caagctgact gtttgctcga gcaggagaaa
    11341 ttgctcttcc gcttcggcat cgtcccacgt ctgatccgat gtgggatagg gtcgaatgac
    11401 gatgctctcg ccttcatgtg gaatggtctg ccagatttct tccgtgatga atggcataaa
    11461 cggatgcaag agccgcatgg tgacttcgag cgtctcaacg agggtctgcc gcgtcaccgg
    11521 tccgtccgcg tgggaggggt tctgcaagac cggtttgatc aattctacat accagtcgca
    11581 gaactcgtgc cagatgaaat gatagagcgc gctcgcagcg cggtcgaagc gataggcctc
    11641 gagctcggag gtgacggtga tgatcgcgtg attaaggcgg ctcaagatcc atcggtctga
    11701 aaacggtcgt tctgacggac gcgccgccgt tcgtgctcca tcgaggtaca tcaatccgaa
    11761 gcgggctgca ttccaaattt tgttggcaaa attacgatag ccttcgatgc gttcttctgc
    11821 cagtttgaga tcccggccgg gcgacgccat cgaggccagt gtgaaccgca aggcatcggt
    11881 gccgaactgg tccatcacat gtaacggatc aatgacgttg ccttttgact tgctcatctt
    11941 ctgcccctcg gcgtcccgca cgagggcatg gatgtaaacg tcgcggaatg gtacgtcgtt
    12001 cataaagcgg aggccgagca tgatcatgcg ggcgacccaa aagaacagga tgtcgagtcc
    12061 ggtcacgagc gttgatgtcg gatagtaggt cttgagctca ggagtctgct cgggccatcc
    12121 cagagtggaa aacggccaca gtccggagga gaaccaggta tccagcacgt caggatcgcg
    12181 cagccactgc tccccgccgc actgcggaca cttggccggc gtggcccgtg cgacgaccgg
    12241 tgtggcacct tgaaggaggg tgatgcgtgt gacgacatcg ccggcttggt tggtggacgt
    12301 ctgccgatgg atgaatcgct cgttgcagtg ctcgcagtac caggcgggga tctggtggcc
    12361 ccaccatatt tggcgtgaga tgcaccagtc cttgatgtca cgcatccagc cgagataatt
    12421 gttaatccat ccctcgggaa tgatgcggat gcggccttcc tcgacggctc ggatcgccgg
    12481 ctctgccagc ggcttgatct cgacgaacca ttgaggcgag agatag
//
LOCUS       DASTQY010000268         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1630569, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000268 DASTQY010000000
VERSION     DASTQY010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630569"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VFR82_08895"
     CDS             complement(<1..1178)
                     /locus_tag="VFR82_08895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEU4501754.1"
                     /translation="MTDSPTIRSAMVFFEELLHGCETPNIAVRFWDGSVWRLREDQRI
                     EATLILRHPDSLSRMLRWPVQLALGEAYIYDDIDVLGSLDALLPLADHLMKRRWSLMD
                     WLRCGVATWRQRSLDRLPEETRNLALHGRRHEKPRDKAAIRFHYDVPVEFYRLWLDSR
                     LLYSCGYYQHEDDDLEQAQTQKLEYLCRKLRLKPGDRILDIGCGWGGFALYAAQTHGA
                     LVHGITLSRRQAEVANHRIAAAGMSRRCRIEVRDFRDVHGDQLYDKIISIGMVEHIGK
                     NQLAPYYERASGLLSPGGVFLNQGIGTRDGEPGLGAFANRYVFPDAELVPIQDIVRAA
                     ECSGFEVCDVESLRQHYALTLDAWGQRLERHHQDALRAVGEVTYRVWRAYMAMAAYLF
                     R"
     gene            complement(1269..>2964)
                     /locus_tag="VFR82_08900"
     CDS             complement(1269..>2964)
                     /locus_tag="VFR82_08900"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEU4501755.1"
                     /translation="PYWKDKCLYFSYEGGDRIRRSLAVHFYPLPTQVKGNSVQFDITL
                     KSNETKQIRISLALAESEEQPNRLFAQTSVYRDLHAITQGIKASCEQWMRRETEFHSD
                     SLLLNRVMRRSLMDLHMLKTSIGGDEFFAAGVPWYVTLFGRDCLITSLQTLAFWSDTA
                     AATLRLLARYQGAEVNDWRDEEPGKILHELRVGELARMNKIPHTPYYGTIDATPLFLI
                     LMSRYAHWTGDLALFEELRIPIERALHWIDKYGDIAGDGYVSYVGNTNKGLLNQGWKD
                     SGDAIVTEDGRLAEPPIALVEVQSYVYMAKMEIADLYERVGEREHAARLRAEAGALAA
                     RFNHDFWVEEEGCFALALEAERRPCRVMSSNAGHALWTGIADKQKAERVVHRLMRPDM
                     FNGWGIRTLSYKDRRYNPMGYHLGTVWPHDNSLIAAGFRRYGYQEEASRIFVGLLEAA
                     MEFEDYRLPELFTGFGREEYGQPVRYPVACHPQAWAAGAIPFLLETFLGIIPDAFNTR
                     LKVVKPFLPEFINQVELRHLRIGKASVDLRFERRQDGTLHAHVGQVTGDLRVEVEG"
BASE COUNT          622 a          915 c          839 g          588 t
ORIGIN      
        1 cggaacagat aggccgccat ggccatatag gcccgccaga cgcgataggt cacctcgccc
       61 acggcgcgca gggcgtcctg atgatgccgt tccagtcgct gtccccaggc gtcgagcgtg
      121 agggcatagt gctgccgtaa actctcgaca tcgcacactt cgaagccgga gcactcagcc
      181 gcacgcacga tgtcctgaat aggcactaac tccgcgtccg gaaacacgta ccgattggca
      241 aacgcaccga ggcccggttc tccatctctg gttccaatac cctgattcag aaacacgccg
      301 cccggtgaga gtaatccaga agcacgctca taatagggcg ccagctggtt cttcccgata
      361 tgctccacca tgccgatgct aatgatcttg tcgtacaatt gatcgccatg cacgtcccga
      421 aagtcgcgta cttcgatgcg gcagcgccgg gacatcccgg cagccgcgat ccgatggttg
      481 gccacctcgg cttggcgccg gctcaacgtg attccatgta ccagcgcccc gtgggtttga
      541 gccgcataga gtgcgaagcc tccccagccg cagccgatgt ccagaatccg atctccgggt
      601 ttgagccgca acttccggca caggtattcc agcttttggg tttgtgcctg ctccaggtcg
      661 tcatcctcat gctgataata gccacaggag tacagcagtc ggctgtccaa ccagagccga
      721 tagaactcca ccggcacatc gtagtgaaac cgtatggccg ccttgtccct aggcttctca
      781 tggcggcggc catgcaaggc gaggtttcgc gtttcttccg gcaggcgatc gaggctccgt
      841 tgacgccacg tagccacgcc gcatcgtagc cagtccatca gcgaccagcg gcgcttcatg
      901 agatggtctg ccaggggcaa gagcgcatcg agcgagccga gcacgtcgat gtcgtcatag
      961 atgtaggctt cgcccagcgc gagttgaacg ggccagcgga gcatgcggct caaggaatcc
     1021 ggatgacgca agatcagtgt cgcctcaatc cgctggtctt cccgcagccg ccagaccgac
     1081 ccatcccaga aacggacggc aatgttcggc gtctcacagc cgtgaagcag ttcctcgaag
     1141 aagaccatcg ctgaccggat ggtgggcgaa tcggtcatgt ccggagcctc atcctcatcc
     1201 gggggagaat ccttcatatg ggatagcata gtgacaacct ccgggccggc ggtgcgacgc
     1261 acctgtcctc agccctcgac ctccacgcgt aaatcgcccg tcacttgtcc aacatgcgcg
     1321 tggagagtgc catcctgcct gcgttcaaac cgcagatcta cggaggcctt gcctattcgt
     1381 agatggcgga gctccacctg gttgatgaac tcaggcagga aaggcttcac caccttcaat
     1441 cgcgtgttga aggcatcagg aatgatgcct aagaatgtct caaggagaaa cggaatcgct
     1501 ccggccgccc atgcctgcgg atggcatgcg acgggatagc ggacaggttg gccgtattcc
     1561 tcgcgcccaa accccgtaaa caactccgga agacgataat cctcgaactc catggccgct
     1621 tcgagaagtc ccacaaagat acggctggcc tcctcctgat atccataacg tcggaacccg
     1681 gctgcgatga gggaattatc gtggggccag acggtgccca ggtgataccc catcgggtta
     1741 tagcggcgat ctttgtagga caacgtgcgg atcccccacc cgttgaacat atcgggcctc
     1801 atgaggcggt gaacgactcg ctcggccttc tgcttgtccg caatgccggt ccacagcgca
     1861 tgtccggcat tggaggacat cacccggcaa ggacggcgct ccgcttccag cgccagcgca
     1921 aaacagcctt cctcctccac ccaaaagtca tgattgaagc gtgcagccag ggcaccggct
     1981 tccgcccgca aacgggcagc gtgttcgcgc tcaccgaccc gttcatagag atcggcaatt
     2041 tccatcttgg ccatgtagac atagctttgc acttccacca gggcaatggg cggctcggcc
     2101 aatcgaccat cctcggtcac gatggcatcc ccggagtcct tccacccttg attgagcagg
     2161 cccttgttcg tgttgcccac atagctgaca tacccgtcgc cggcaatgtc gccatacttg
     2221 tcgatccaat gcagcgcgcg ttctatggga atgcgcaact cttcaaacag ggcgagatcc
     2281 ccggtccaat gtgcgtagcg gctcatgagg atgagaaaga gcggcgtggc atcgatggtg
     2341 ccgtagtacg gcgtgtgcgg gatcttattc atccgcgcca actcgccgac gcgcagctcg
     2401 tggaggatct ttccgggttc ctcatcccgc cagtcattga cctctgctcc ctgatagcgc
     2461 gcgagcaagc ggagtgtggc cgcggccgtg tcagaccaga aggccagtgt ctgcagcgac
     2521 gtaatgaggc agtcccgtcc gaataacgtg acgtaccagg gcacgccggc ggcaaaaaat
     2581 tcatcgccgc caattgatgt tttcagcatg tgcagatcca tcagcgatcg gcgcatgacg
     2641 cggttgagca acagactgtc gctgtgaaat tccgtttcac gcctcatcca ttgctcgcag
     2701 gacgctttga tgccctgagt gatcgcgtgc aaatctcgat acacgctggt ctgtgcgaac
     2761 agccggtttg gctgttcctc cgattctgcg agggcaaggg agatacggat ctgcttggtc
     2821 tcattcgact tgagcgtgat atcaaactga acggagttgc ctttgacctg ggtgggaagg
     2881 ggataaaagt gcacggcgag actcctgcgg atgcgatcgc ctccttcata tgaaaagtag
     2941 agacacttgt ccttccagta gggc
//
LOCUS       DASTQY010000269         4106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_841570, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000269 DASTQY010000000
VERSION     DASTQY010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841570"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VFR82_08905"
     CDS             <1..906
                     /locus_tag="VFR82_08905"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU4501756.1"
                     /translation="VLIGSIIFVFGSFILMIGMMLWESNKAYKMKELARTVQTDSRPA
                     ASTLPAQDYSMYKTKMGDEGREMVQIPEGPFTMGSQDGDPDETPEHQVFLKGFFLDRK
                     EVTQEEYMRFAKMTKRGVPRIEVFEDDQSKLLKPEFAAMSVSWDDALAYCRWAGKRLP
                     TEAEWEKAGRGEGKRKYPWGDKFISNGANVDGSEDGYKYLAPPGSFDAGRSPYGLYDM
                     TGNVAEWVNDTYAENYYKKSPYRDPKGPEGGGDLRVVRGGSWRETEANARLSKRFTAK
                     HWRTDITIGIRCASDLDQGGTAPTS"
     gene            916..1818
                     /locus_tag="VFR82_08910"
     CDS             916..1818
                     /locus_tag="VFR82_08910"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU4501757.1"
                     /translation="MLETKFKLALLLIVLAFAALPVMGILRGTTTTPFEDAPPGTDES
                     SLSELESTSSQEEPIQDDMVTIPAGPFLRGTTSGGFDEQPQRSIDLDTFSIDRHEVTN
                     HHYQQFALATGHRKAGPPSRYAKSIGRMRGANQPVVYVSWDDAAEYCRWKGKRLPTEA
                     EWEKAMRGPDGRLWPWGDQEKPNGANWARVQDGYEVTARVGTFQTDRSPYGVMDGAGN
                     VMEWVADWYQEAYYKESPDKNPPGPEYGTYRVMRGGAYTTSGVDLRITSRSKMVSDFR
                     DETIGFRCATSGVFPGVRSTSERS"
     gene            2051..2182
                     /locus_tag="VFR82_08915"
     CDS             2051..2182
                     /locus_tag="VFR82_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501758.1"
                     /translation="MATATATAPDSEIKIKIGKMIFYITCAIGLWFFYWFAGIQCPC"
     gene            2251..2373
                     /locus_tag="VFR82_08920"
     CDS             2251..2373
                     /locus_tag="VFR82_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501759.1"
                     /translation="MGQTIFYILISTVICVAYFTLVDHYLMDMQGLDYWYLFRK"
     gene            2452..>4106
                     /locus_tag="VFR82_08925"
     CDS             2452..>4106
                     /locus_tag="VFR82_08925"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEU4501760.1"
                     /translation="MGPVTAKKVLSIMALCAMVTLLVLPVVVSLPSPAAGEDAAAVDG
                     AKKDGDKVEKGRDVYYKTEGIVVGAPAPKTTDGPKDYPRYNFESRVLLWFANQQHLYY
                     GSFVLAVPIFCMVIEFMGVVSKDKALAKRYDQLAYDFVKISLTAYSLTAILGGILIFT
                     FLTLYPAFFGYLSSIFRPVMHIYALMFVAESGTLYIYYYGWDKMKEGFLKWIHLSMSV
                     VLNIIGTLLMFLANSWIGFMMSPAGVDEQGRFLGNIWHVIHTALWNPLNVHRILGNMA
                     FGGGVVAAYAAYKFLASKTDEDRAHYDWMGYIAMAIGVAFLIPLPFAGYWLMREVYAY
                     RQQMGITLMGGLLAWLFIIQATMIGILFLSSNYYLWQALGRMRGAEKYQRYIKYLVFL
                     LVCGFLVFITPHTMVMSPAELKAMGGQQHPVLGNYGVMSAKNGGINVIITTTVLSFVW
                     YMRGNKVSTVSWSKFGNIFMGCFFFWAYVNIIWLAIYGYYIPANVRVGLSVPQVATTL
                     SCLFFMFALNSAMMKGAKQMGPIEWGKISARSQYALIMLATAFT"
BASE COUNT          937 a         1056 c         1174 g          939 t
ORIGIN      
        1 gtgttaatag gatcgatcat cttcgtgttc ggttccttca tcctcatgat cgggatgatg
       61 ctgtgggaat cgaacaaagc ctataagatg aaagaactcg cgcgaacggt gcaaacggat
      121 agccgtccgg ctgcgagcac ccttccggca caggattatt cgatgtacaa gaccaaaatg
      181 ggagatgaag gccgcgaaat ggttcaaatt cccgaaggac ccttcacgat gggcagccaa
      241 gacggcgatc ccgatgagac gccggagcat caagtgtttc tgaagggctt tttcctcgat
      301 cgaaaggaag tgacgcagga agaatacatg cggttcgcca agatgactaa gcgtggagtt
      361 cccaggatcg aggtgtttga agacgaccag tccaagctgc tgaaaccgga atttgcagcc
      421 atgagcgtct cctgggatga cgcgctggcc tattgccggt gggcagggaa gcgtcttcca
      481 accgaagccg agtgggaaaa ggccggacgc ggtgaaggga aaagaaagta tccttggggc
      541 gacaagttca tcagcaacgg tgcgaacgtg gacggcagcg aagacggata caagtatctg
      601 gccccgcctg gctcgttcga tgccggccgg agtccgtatg ggttgtatga catgacgggc
      661 aacgtcgccg aatgggtgaa cgacacctac gccgaaaatt attataaaaa atccccctat
      721 cgggacccga agggcccgga aggcggggga gatctgcgag tagtccgcgg cgggtcgtgg
      781 cgtgaaaccg aggccaatgc acggttgtcc aagcgattta ctgcgaaaca ctggcgcacg
      841 gatattacca tcggcattcg ttgcgcgagc gatctcgatc agggaggcac tgctcccact
      901 tcataatcag ccggcatgct cgaaaccaaa ttcaagctcg cccttctgct aatcgttctc
      961 gccttcgcag cgctcccagt gatgggcatt cttcgcggaa cgacaaccac accctttgag
     1021 gacgccccgc ctggcacgga cgaatcaagc ctctcggagc ttgagtcgac atcgtcacag
     1081 gaagaaccga tccaagacga catggtgacc attccagcag gaccatttct tcgtgggacg
     1141 acgagcggcg gatttgacga acaaccgcag cgaagcatcg acctcgatac gttctccatt
     1201 gaccggcatg aagtcacgaa ccaccactat cagcagtttg cccttgccac cggtcatcgc
     1261 aaagccggac cgccttctcg ctatgcgaag agcatcggtc gtatgcgcgg ggcaaaccag
     1321 cctgtcgtct atgtgtcctg ggatgatgcc gccgagtatt gccgctggaa aggcaagcgg
     1381 cttccgacgg aagcggaatg ggaaaaggca atgcgaggcc cggatggccg gctgtggccg
     1441 tggggagacc aggagaagcc gaacggagcg aactgggctc gggtgcagga tggatatgag
     1501 gtgacagcgc gggtcggcac gtttcaaacg gataggagcc cgtatggtgt gatggatggc
     1561 gccgggaacg tcatggaatg ggtggccgat tggtaccagg aagcctatta caaggaatct
     1621 cctgacaaaa atcctcctgg tcctgaatac gggacttata gggtcatgag agggggagct
     1681 tacaccacct ccggagtcga ccttcgcatt acgagtcgca gcaaaatggt gtcggatttt
     1741 cgggatgaaa ctataggatt tcgctgtgcg acttcagggg ttttcccggg ggtccggagc
     1801 acgagcgaga ggagctaaac tctacaaaaa atcaaagtaa tagaggatca gaaacacggc
     1861 caaaatgata ttgacaacca tgccagccaa aactataatg tcgaacactt ttgagttttt
     1921 attcatgctt ttcctgtgtt tccacgccca acgacaaaat ttgaataaca cacgcatggg
     1981 tgggtgtcaa gaaagagcct gatgtaattt cacgtatttt tcttattctt caagtagaga
     2041 aggagacacg atggcaacgg caaccgcaac cgctccagac agtgagatca aaatcaagat
     2101 cggcaagatg attttctaca tcacctgcgc gatcggtctg tggtttttct attggttcgc
     2161 cggtattcag tgcccctgct gaacagccag ggccgccgaa cgctctttag tcacttactg
     2221 tttggttact gagttgagga ggagatgact atgggtcaga cgatttttta tattctaatt
     2281 tcgaccgtga tctgcgtcgc ctacttcacg ctggtcgatc attaccttat ggatatgcag
     2341 gggctggatt actggtatct cttccgcaag taatagcagc ggagtcgggc ctcacgcttg
     2401 agttcctgga atgttgcaga tttttatcgg aatgcatcaa ggaggtatcc catgggtcct
     2461 gtaaccgcca agaaggtgtt gtcaatcatg gcgctctgcg cgatggtgac ccttctcgtg
     2521 cttcctgtgg tcgtctcgct tccctcgccg gcagccggtg aggacgcggc ggctgtggat
     2581 ggagcaaaga aggatgggga caaggtcgag aaggggcgcg atgtgtatta caaaactgag
     2641 ggcatcgtgg tcggtgcccc ggccccgaag acgacggatg gtcccaaaga ctatccgagg
     2701 tataacttcg agagccgcgt gttgctctgg tttgcgaacc agcagcacct ctactacggc
     2761 agcttcgtgc tggccgtgcc gatcttctgt atggtgatcg agttcatggg ggtcgtgagc
     2821 aaggacaagg cgctcgcgaa acgatatgat cagctggcct acgactttgt gaagatcagt
     2881 ctcacggcct actctctgac ggcgatcctg ggcggcatcc tgatcttcac cttcctgact
     2941 ctctatcccg cgttcttcgg gtatctatcc agcatcttcc gacctgtgat gcatatttat
     3001 gcattgatgt tcgtagccga gagcgggacc ctttacatct actattacgg ttgggacaag
     3061 atgaaagagg ggtttttgaa gtggatccat ttgagcatgt cggtcgtctt aaacatcatc
     3121 ggaactctgc tgatgttcct agcgaattcc tggatcggtt ttatgatgtc gccggccggt
     3181 gtcgatgagc agggaaggtt tttgggcaat atctggcacg tcatccatac cgcgctatgg
     3241 aatcctctca acgtccaccg cattctcggc aatatggcgt tcggtggcgg tgtcgtggcc
     3301 gcctatgccg cctataaatt cctcgcatca aagaccgatg aggatcgggc ccactacgac
     3361 tggatgggct atatcgccat ggcgatcggt gtcgcgtttc tgattccctt gcccttcgcc
     3421 ggctactggc tgatgcgaga ggtgtatgcc tatcgtcagc agatgggcat caccctgatg
     3481 ggtgggttgc tggcctggtt gttcatcatc caagccacca tgatcgggat cctcttcctc
     3541 agttccaact attacctctg gcaagcactc ggccggatgc gcggtgcgga gaaatatcaa
     3601 cggtacatta agtatctcgt ctttctgctc gtctgcggct tcctggtgtt tatcaccccc
     3661 catacgatgg tcatgagtcc cgccgagcta aaggccatgg gcggccaaca gcatccggtg
     3721 ctcgggaact atggtgtgat gtccgccaaa aacggcggca tcaacgtcat catcacgacc
     3781 acggtgttga gtttcgtgtg gtacatgcgg ggcaacaaag tgtcgacggt gtcgtggtcg
     3841 aagtttggaa acatcttcat gggatgtttc ttcttctggg cctacgtcaa cattatttgg
     3901 ctcgcgatct acggctatta catcccggcc aacgtccgcg tcggactctc cgttccacaa
     3961 gtcgcaacca ccctgtcgtg cctgttcttc atgttcgcgc tcaatagcgc catgatgaaa
     4021 ggcgccaagc aaatggggcc aatcgagtgg ggcaagattt ccgcccgttc gcaatacgcc
     4081 ttgatcatgc tcgcgacggc ctttac
//
LOCUS       DASTQY010000270         3341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_376550, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000270 DASTQY010000000
VERSION     DASTQY010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376550"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VFR82_08930"
     CDS             <1..705
                     /locus_tag="VFR82_08930"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEU4501761.1"
                     /translation="VVPRSFAIKVKTGQSLNQIRFVRGEAAASDAALQHLHRQEPLLY
                     HNLSDRKPLRQREFRTDRGLFLRIDLKGDERTDSKVIGYRAKKNSHVIDLSKIGYYAA
                     TDFWEPLYRHRQDSLLLEPEEFYILASKERIRVPPDYAAEMVAYEAACGELRTHYAGF
                     FDPGFGYGADGELKGTQVVLEVRPHDVPFLIHDGQTFFKVVYDRMLDVPERLYGVGLG
                     SSYQQQALTLSKHFKA"
     gene            805..1221
                     /locus_tag="VFR82_08935"
     CDS             805..1221
                     /locus_tag="VFR82_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501762.1"
                     /translation="MAPTDLPTPQDPQRSPPKPPPSPSDSQESSGDHQMRVIGLMVGT
                     ALMFIGFLNVFLSISGGFEINVVPFLIYFGGLAVWANAAIEVPAVRYGVMAAAIVLAL
                     GFFHYGEVLFWHKQVVFWATVVIVMYFMFNEPKKPG"
     gene            1248..2024
                     /locus_tag="VFR82_08940"
     CDS             1248..2024
                     /locus_tag="VFR82_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEU4501763.1"
                     /translation="MSIPQTSSSSRWEQAAHLGHRGDTVTIAVIIPTLNEASVIERTL
                     QLTRQLGFEEIIVVDGGSTDRTNVVVDTIAGHPNAGLTPVRRLTAPAGRSRQLNAGAR
                     SCRQDVLLFLHADSHLPVDAKWLIECALADPEVAGGRFDLRFDRPSMWGRIISLFMNL
                     RSRLSRISTGDQAMFVRRHVFQQLDGFPDIPIMEDIEFSARLKRTGHTVALRSYVTTS
                     FRRWERQGPLRTILLMWALRFLYWIGISPHRLARLYADVR"
     gene            2044..2781
                     /locus_tag="VFR82_08945"
     CDS             2044..2781
                     /locus_tag="VFR82_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEU4501764.1"
                     /translation="MTTQPQPARTALVIFAKAPVPGQVKTRLCPPLTADEAATIHGTF
                     VLDTLERTKAAVTKLKLHADRYLACAPSSTLAFFRILEERHAVDLIEQEGDDLGARMN
                     RACETLFQRGYQQVLIVGTDVPSLPLDYYRQALVQLERHDVVVGPALDGGYYMIGLKK
                     IAPALFHGIPWSTDRVLTMTREKAIDMGLLVALLPEWRDVDTIDDLRLLIEASILDAG
                     KPKQEQSFSNRTAGALQLIAKRLRSRT"
     gene            2799..>3341
                     /locus_tag="VFR82_08950"
     CDS             2799..>3341
                     /locus_tag="VFR82_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4501765.1"
                     /translation="MTTKVALITGGARGIGRGIALDLASQQWHIAFCYRTSEAEANDT
                     AAAIIQRGGQASAIRCDVSDPVAAKEMVALVEKQWGHIDALINGAGPYHRVNLFEETI
                     TGWNDMFEGNLHPIFYLAQAAAPGMKARRSGRIINFSMANADHMIAQPDVTAHYIAKA
                     GVLILTRTLAKLLAPHGITVN"
BASE COUNT          720 a         1015 c          905 g          701 t
ORIGIN      
        1 gtcgtcccgc gttccttcgc catcaaggtc aagacgggtc aatcgctcaa tcaaatccga
       61 tttgtgcgcg gcgaagcagc agcctcggat gccgccctgc agcatctgca tcgtcaagag
      121 cccctcctct atcacaacct gtcggaccgc aagcctcttc gccaaaggga attccgtacg
      181 gatcgcggtc ttttcctgcg catcgacctc aagggcgacg agcgcaccga ttcgaaagtc
      241 atcgggtacc gagccaagaa aaacagtcac gtgatcgacc tctccaaaat cggatactat
      301 gccgccacag atttttggga accgctctac cggcatcggc aggacagtct gctcctcgaa
      361 ccggaagaat tttacatcct tgcgtcgaaa gaacggattc gtgtgccgcc ggattacgcc
      421 gctgagatgg ttgcctatga agcggcctgc ggcgaactcc gaacgcatta cgccggattc
      481 ttcgatccgg gattcggcta cggagccgac ggcgaactga agggcaccca agtggtgctc
      541 gaagtacgcc cgcacgacgt ccccttcctc atccatgacg gtcagacgtt ttttaaagtc
      601 gtctatgata ggatgctcga cgtgcctgag cggctctacg gcgtcggtct cggctcttcc
      661 tatcaacaac aagccctgac tctcagcaag catttcaagg catgaagcag tctgttttcg
      721 agcctcagca atctggaatg cggcgcaccc ttgtatgcgc ctctggcgaa tgaccggttg
      781 accagcgact cacggtaacc ccccatggca ccgaccgatc tccccactcc ccaagacccg
      841 caacgctccc cccccaagcc accgccgtca ccgtcagaca gccaagagag cagcggtgat
      901 catcaaatgc gcgtcatcgg cctgatggtc ggaacggcac tgatgttcat cgggtttctc
      961 aatgtatttc tgtcgatcag cggtgggttc gagatcaacg tcgtcccgtt tctcatttat
     1021 ttcggcggtc tcgcggtatg ggccaatgcc gcgatcgaag tgcctgccgt gcggtacggc
     1081 gtcatggctg ccgccatcgt cctggccctg ggattttttc actatggcga agtgctgttc
     1141 tggcataagc aagtagtctt ctgggcaact gttgtcatcg tcatgtactt catgttcaac
     1201 gagccaaaga agccgggcta aacgatcgat cggccggcct ggaggcgatg tccatacccc
     1261 aaacgtcctc ctcatcccga tgggagcaag ccgcccacct tggtcatcgt ggtgacacgg
     1321 tgacaatcgc cgtcatcatc cccactctca acgaagcttc ggtcatcgag aggaccctcc
     1381 aactgacgag acagcttggc ttcgaggaga tcatcgttgt agacggcggg agcaccgatc
     1441 ggaccaacgt cgttgtcgac acgatcgccg gccacccgaa tgcaggctta acgccggttc
     1501 ggcgtctcac cgctcccgca gggaggtcga ggcaattaaa tgcaggcgcg cggtcctgcc
     1561 ggcaagacgt cttgttgttt cttcacgcag acagccatct gccggttgat gccaaatggc
     1621 tcatcgaatg cgcgctggcc gatccggagg tcgcgggtgg acggttcgac cttcgatttg
     1681 accgtccgtc gatgtggggt cgtatcatca gcctattcat gaacctgcgt tctcggctca
     1741 gtcgcatctc gaccggcgac caagccatgt tcgttcgccg tcacgtcttc caacagctcg
     1801 acggctttcc cgatattccc atcatggaag atatagagtt cagcgcccgt ctgaaacgga
     1861 ccggccatac agtcgcgctc agaagttacg tcaccacctc gttccgtcga tgggagcggc
     1921 agggcccttt gcgaacgatc ctcttgatgt gggctttgcg cttcctgtat tggatcggca
     1981 tcagtcccca ccgactggcg agactttatg ccgatgtgag atgaacaacg gtacatggac
     2041 cgcatgacca cccagcccca accagcccgc acggcattag tcattttcgc taaggcgccg
     2101 gtgcccggcc aggtcaagac gcgcctttgt cctccgctca cggcagatga agcagcgacc
     2161 atccatggga ccttcgtgct cgatacgctc gaaagaacga aagccgccgt cacgaaatta
     2221 aagcttcacg ctgatcggta cctggcctgt gccccatcgt cgacattggc attcttccgg
     2281 atcttggaag agcggcacgc ggtggactta atcgagcaag aaggagacga cttaggcgcc
     2341 cggatgaatc gcgcctgcga gacacttttt cagcggggct atcagcaggt cttgatcgtc
     2401 ggcacagacg tgccctcgct tcctctcgac tattaccggc aggctctcgt tcagttggaa
     2461 cgccacgatg tggtcgtagg cccggccctc gacggcggct actacatgat tggactcaag
     2521 aaaatcgcgc ccgcgctgtt tcacggcatc ccctggtcga ctgatcgcgt gctcacaatg
     2581 acgcgtgaaa aggcgataga catgggactt ctggttgcgt tgcttccgga atggcgcgat
     2641 gtcgatacca tcgatgatct acgcttgctg atcgaggcaa gcatccttga cgctggaaag
     2701 ccaaaacagg aacaatcgtt ctccaaccgg acagccggag cattacaact cattgccaaa
     2761 cgactgcggt caagaacgta aacgtaaagg gatgacctat gactacgaag gtcgcattga
     2821 ttacaggcgg agcgagaggc ataggacgag gaatcgcttt ggatttggcc tcacagcaat
     2881 ggcacattgc gttttgctac cgaaccagtg aagccgaggc caacgacacg gccgctgcca
     2941 ttatccagcg aggcggccag gcctcggcga tccgctgtga cgtatcagac cccgtcgcgg
     3001 ccaaagagat ggtcgccctg gttgaaaagc aatggggtca catcgatgca ttaatcaacg
     3061 gtgcggggcc ataccatcga gtcaacctgt ttgaggaaac catcaccggc tggaatgaca
     3121 tgtttgaggg gaacttgcat cccatttttt atctcgcgca ggcagccgca cctggtatga
     3181 aggctcgccg gtccggccgt atcatcaatt tcagcatggc caatgccgat cacatgatcg
     3241 cccaaccgga tgtgaccgcc cactatatcg caaaagccgg cgtgctgatt ctgacacgca
     3301 cgctcgcgaa gctgctggca cctcatggca tcaccgtcaa t
//
LOCUS       DASTQY010000271         5910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1683640, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000271 DASTQY010000000
VERSION     DASTQY010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683640"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..2788)
                     /locus_tag="VFR82_08955"
     CDS             complement(122..2788)
                     /locus_tag="VFR82_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU4501766.1"
                     /translation="MTEKQFVTLVRRLEVYSQHYPIRYKIKVALLGVAGYAYVLFVLG
                     LVTGLSFGCFLLIRDGHSGGGALFKVGAGLVILATVIARALWVRLTPPTGIELHREDA
                     PRLFLLIEKLTTALQTPPFHHVLIVNELNAAVVQTPRLGIFGWQRNYLLLGMPLMLTF
                     TPAQFRAVLAHEFGHLGRQHGRFFGWIYRARVTWWRLLQEMTARQHWASFVFTKFLGW
                     YAPFFNAYSFVMARAQEYEADRNAALLVGPHCMKDTLASLAVIGRMLQESFWPSLDQK
                     TLESETPPSSYLTELTLTVGSEAQRDRASSFFAEEINAKTGYADTHPSLGDRLAALNK
                     LPTKKPSSSQSSIPQLLGPGETHAAQYYLGSNCGTLCEQLDREWVESVKDTWKEKHEE
                     CVAAQARLHALTGKAATESPTVGEWWEYAQLTERFEGGRAALAIIDDMLAHFPEHAPA
                     LFAKGRLLLSQGDQKGVTFLDQATTLDQEALLSACDIAERFYERAGRHKEAAAYRNRA
                     IERRDMMVAAEQERQNISPKDSFFRHRLPEEATTALAHELSAFSEIHEAYLVQKRVQV
                     LPDVPAHVLLVVPRYRWYQSEDARNETLLSRLTQEVTLPSSTYVLVLGSRLKRRWKKT
                     LRGLQPLALSGGSEKNVLSKNQSAADHRSTMSRREVWSSWLRRRAPEFLVVAIVIAIF
                     LNYTGHPEPNCTDHPTGITAKPVGSRTLYLVPIGEFPLATLSRLSAHYAHAWHVPITT
                     VRPLPLDESLWDRTRDQLVAERIVGKMKRQYADLLAQSDTILIGLTAHDMYRQQSSVR
                     YSFSHREDRRYAVVSSARMHPRTLPLHLPHGSLFSLDQRDEDLAQCRLTKMVGKNVGM
                     LYYHLAMNDDPTSLLYNNIRGPGDLDRVTEQF"
     gene            3130..3888
                     /locus_tag="VFR82_08960"
     CDS             3130..3888
                     /locus_tag="VFR82_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501767.1"
                     /translation="MENAYKNFFEAECRELADLETFDPDAFVGDDAVPQSVCNFVLSL
                     ALIFNDLRDLYYANFLVGGCKPTGSVLKNKQWGYYSGLYLHIVRLILSAIHELLNLIE
                     KQRADMDHPIFADVLKALPKDKRKEWFDVVGIALGKSSADSRLEKLLLRIRNNVSFHY
                     DAKALYGGFRHHFLVHQPEERAYVSRGNTVQSTRFYFADAAVDGLLNEISGTNGPDLA
                     ASFEKLIDGVYQPVMLIISGFIQKRGCAFRQEPC"
     gene            4074..4454
                     /locus_tag="VFR82_08965"
     CDS             4074..4454
                     /locus_tag="VFR82_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4501768.1"
                     /translation="MYPFGHIDLRARDLQAVYPFYSRILPELGFWKEWRGEGAFSFQA
                     EGKLPKLSWFGVIEDHHHIPNATRLAFAVESRKEVDRLADIVRAAGAINIEGPEDMPY
                     ARIYYAVYFEDPSGNRLEVYYCEE"
     gene            complement(4666..>5910)
                     /locus_tag="VFR82_08970"
     CDS             complement(4666..>5910)
                     /locus_tag="VFR82_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501769.1"
                     /translation="FFQATDGGQRTVHMGQRVHATLKGHVGDDGKLQYIDVEAVVVIM
                     RGGSGLPTTMRRARAVFRFTPDRNLPVLPTNIVQQTAEAWNFEDADPRDIDLSQTLLA
                     SVMAYGGPNMLQAETKWNDPKTGCAEIRFAPVTKQQRVGRNQSTKVRAEVWAKESPAA
                     VPGQFRYAIALPEGLGQVSPKQIDTKPDTVAPFTYTAPATRVRHRGFKVGAISRAGAA
                     EAQWEATDLSYVLEFRSTVVGKDDIEPAQSQASATIRLEPQPDTPAGSAAKYAGDGQV
                     AYQTSPLPNWDSCKPLIRGQGTVPIRVHQALIHVEDDARTAKIEMVYALLGVSHETNT
                     GWAYMLNFKCVLNPAEFHPYWTSMYVSGRAEAGASAEKMFTLKDWMYVGRDVVVATKT
                     LRSTCGGECADEVATFTLKEED"
BASE COUNT         1305 a         1571 c         1691 g         1343 t
ORIGIN      
        1 agctagcgac ggctgtaaat agcaccttct tcacgagggc ttctcctgcc aagctggccg
       61 ctgtagtagc gacgacgcgt acgtgacatg atgaggcagc agaaatgacg ccggcatttg
      121 gctagaattg ctctgtgacc cggtcgaggt cgccgggtcc gcggatattg ttgtatagaa
      181 gactcgtcgg gtcgtcgttc atggccaagt ggtaatacaa cattcccacg tttttcccga
      241 ccatttttgt aagtcggcac tgtgccagat cctcatcacg ctgatccaac gagaagagag
      301 agccgtgcgg taggtgaagg ggaagcgtcc gaggatgcat ccgggcactg gacactacgg
      361 cgtatcgtct gtcctctcga tgcgaaaagg aataccgtac tgacgattgt tgccgataca
      421 tgtcatgagc cgtcaggcca atcaggatcg tatcggattg agcaagcaag tcagcatact
      481 gccgcttcat ttttccgacg atgcgctcag cgacaagttg atcacgcgtc cgatcccaga
      541 ggctctcatc caagggaagc ggacggacag tggtgattgg aacatgccag gcatgggcgt
      601 aatgagcgct caatcgcgac agcgttgcta gcgggaactc accgatagga accaggtaga
      661 gtgtacgaga tccaaccggc ttggcggtga ttccggtagg gtgatcagta cagtttggtt
      721 ctggatgacc cgtatagttt agaaaaatcg cgattacgat ggccacgact agaaattcag
      781 gggcgcgacg cctgagccaa gaactccaga cttcgcgtcg agacatggtc gatctgtgat
      841 cggcggctga ctggttcttg cttaagacat tcttttccga accgccggat agcgccaatg
      901 gttgcaggcc acggagtgtt ttcttccatc tccgtttcaa acgggatcca aggactagta
      961 cataagtgct gcttggaagc gtaacctctt gtgtcaatcg gctgagtagg gtttcgtttc
     1021 tcgcatcctc tgattgatac caccggtatc gcggaacaac caggagcaca tgcgccggga
     1081 cgtccggcaa gacctgcaca cgtttttgga cgagatacgc ctcgtggatt tctgaaaatg
     1141 cagaaagttc atgagcgagc gctgtagtgg cttcttcagg caatcggtgc cggaaaaagg
     1201 agtctttggg agaaatgttt tgtcgttcct gctccgcggc gaccatcatg tctcgccgtt
     1261 cgatggcacg gtttctgtag gcggcagctt ccttgtgacg gcctgcccgc tcataaaagc
     1321 gttccgcgat gtcgcaggca gaaagcagcg cctcctgatc cagggtcgtg gcttgatcga
     1381 gaaaggtgac gcctttctgg tcaccttggc tcaaaagcag acgacctttt gcgaatagtg
     1441 ccggtgcatg ctcgggaaag tgagcgagca tgtcatcgat gatcgccagt gccgctcgcc
     1501 ccccctcaaa tctctcggtc aattgggcgt actcccacca ttccccaact gtgggcgatt
     1561 cagtagcggc tttgccagtc aaggcatgga gacgcgcctg ggcagcaacg cactcttcat
     1621 gtttctcctt ccatgtatct ttgaccgatt ccacccattc gcggtctagc tgttcgcaca
     1681 gtgtcccgca gttgctcccc agatagtact gcgcggcatg agtctctccc gggccgagca
     1741 gttgagggat tgaagattga gaagagctgg gcttctttgt cggcaattta ttcaaggctg
     1801 ccaggcgatc tccgagggac gggtgcgtgt cggcgtagcc tgttttcgca ttgatctctt
     1861 ccgcaaagaa cgacgatgcc cggtccctct gcgcttctga tcccactgtc aacgtcaatt
     1921 ccgtcaaata tgaggacgga ggcgtttcgc tctcaagcgt cttctgatcc agggaaggcc
     1981 aaaatgattc ttgaagcatc cgcccaatga cagccaacga ggcaagcgtg tctttcatgc
     2041 aatggggccc tacaaggagg gcggcattgc gatcggcttc atattcttgt gcgcgggcca
     2101 tcacgaacga gtaggcattg aagaatggcg cgtaccagcc aagaaactta gtaaagacaa
     2161 acgaagccca gtgttggcga gcggtcattt cctgcaacag tcgccaccac gtgactcgcg
     2221 cgcgatagat ccagccgaag aagcgcccat gttggcgtcc aagatgtccg aattcgtgtg
     2281 cgaggacggc gcggaactgg gccggcgtaa aggtcagcat taatggcatg ccgagcagga
     2341 gataattacg ctgccaaccg aagatgccca gacggggagt ttgaactacg gcggcattca
     2401 gttcattcac gatcaacaca tggtgaaatg ggggagtttg caatgcagta gtcagctttt
     2461 cgattaataa gaaaagtcga ggcgcatcct cgcgatgcaa ttcgatgccg gtcggaggcg
     2521 tgaggcgcac ccagagggca cgcgcgatga ctgtggccag aatcacgaga cccgctccga
     2581 ccttaaagag tgcgccgccg ccggagtggc cgtcacgtat cagaagaaag cagccgaaag
     2641 acagaccggt gaccagtccc aagacgaaga gaacataggc gtagccagca acgccgagga
     2701 gggcaacttt gatcttgtaa cggatcggat agtgttggga ataaacttcc aaacgtcgga
     2761 cgagtgtgac gaactgcttt tcggtcatcg atcgttccgc cagagcacag gagaagaagt
     2821 cgtgttgttg aggaatccgt agctagttta ggagaggagg ccaaattctc aaaatggatt
     2881 gtaaattatg gctcatgggg gtttgagaat ggggtcagga accatatgat tatccgctct
     2941 tactgaccgc catctaatcg gcagtccggt acccgaacct ccgcattcgc gacgcgaggt
     3001 ccactatccc gctggacagc acgcttcagc taaacgagac gaagggggga gcgctgcaat
     3061 cgcttgtaga ccccagcgct atagctggat atattctggc cagcagtgtg ccaaatcgga
     3121 ggagtggaga tggaaaatgc gtacaagaac ttttttgagg cagaatgccg ggagttggct
     3181 gacctggaga ccttcgatcc ggatgcattc gtcggggatg acgctgtgcc acaatcggtc
     3241 tgcaattttg ttctttccct ggctctcata ttcaatgatc tgagggatct ctactacgct
     3301 aactttctcg tagggggctg caaaccgacg gggtcagtct taaaaaataa gcagtggggc
     3361 tattactctg gcctctacct gcatatcgta cggcttattc tgagcgccat ccatgaactc
     3421 ctaaatctga tcgaaaagca gagagcagac atggaccatc ctatatttgc ggatgtactg
     3481 aaagccttac caaaagataa gcgcaaagag tggttcgatg ttgtaggaat tgcgctgggt
     3541 aaatcgtcgg ctgactcgag actagaaaaa cttcttctca ggatacggaa caacgtttcg
     3601 ttccactatg acgcaaaggc cctatatggc ggcttccgac atcacttctt ggtccatcag
     3661 cctgaagagc gcgcctatgt ttcgagagga aacacggtac aatccactcg cttttacttt
     3721 gcggatgctg ccgttgatgg cttattgaat gaaatatctg gaaccaacgg gccggatctc
     3781 gcggccagct ttgaaaaact gatcgatggc gtctatcagc ctgtaatgtt gattattagc
     3841 ggattcattc agaagagagg ttgcgcattt cggcaggagc catgttaatt aggagcagaa
     3901 aaggttcgac tgttccatag cgaaccggcc cccgagttca tcatgccgaa gtgtgctatg
     3961 tcggctggca agaatcgctg aggcttcttg aaaatcgtcg agcgaggatc caaactgggc
     4021 aactgtgtta tgccgtaggg ggtgtctgca atacttaggt cggcaaaaaa agcatgtacc
     4081 ctttcggtca cattgattta agagcacgcg atttacaggc cgtttatcca ttctattcca
     4141 gaattctccc tgaattggga ttttggaaag aatggcgagg agagggcgcg ttttcgtttc
     4201 aggctgaggg gaagctccca aagttgtcat ggtttggagt gatcgaagac catcatcaca
     4261 ttcccaatgc cacgaggctt gcttttgcag ttgaaagtcg taaggaagta gatcggttgg
     4321 ctgacattgt tcgagctgcc ggagccatca atattgaagg gccagaggac atgccctacg
     4381 caagaatata ctatgcagtg tatttcgaag atccttctgg caatcggctc gaagtttatt
     4441 actgcgaaga gtaagtcatc aaaaaggcgc cccgtcagga cggctcatcg ctgctcccgc
     4501 aggcgcgccg caatttcgtg aatccgccgt agcccagccg gcatcgaacg gatggccggg
     4561 cttatcaatg tcgatgacca gcggtgcgtg gtcaaaaggt gtataggttt tcccttccgc
     4621 gcttcagacg cagccactgt tggcaccgaa gcggcttatg gccatttagt cctcttcttt
     4681 cagcgtgaac gtggccacct cgtcggcgca ttctcccccg cacgtgctcc tcagcgtctt
     4741 cgtcgccact actacgtctc gacctacata catccagtcc ttgagcgtga acatcttctc
     4801 cgctgatgct cccgcttcgg cccggccgct gacgtacatg ctggtccaat acggatggaa
     4861 ctcggcgggg ttaaggacac atttgaagtt cagcatgtat gcccacccgg tgttggtttc
     4921 gtggctgacg ccgaggaggg catacaccat ctcgatcttt gccgtacgcg cgtcgtcctc
     4981 cacgtgaatg agtgcttgat ggacgcgaat gggaactgtg ccttgtccac ggatcaacgg
     5041 cttgcaggaa tcccagtttg ggagcggact agtctggtat gcgacctgac cgtcgccggc
     5101 atatttcgcg gcagatccgg ccggagtgtc aggctgtggt tccagccgga tcgtggcgga
     5161 ggcttgcgat tgcgccggct cgatatcgtc cttgccgacg acggtcgatc ggaattccag
     5221 gacgtaggag agatcggtcg cttcccattg cgcttcggca gcgccggccc gcgagatggc
     5281 gccgaccttg aatccgcgat gccggaccct cgtggccggc gccgtgtacg tgaacggcgc
     5341 gacggtgtcc ggctttgtgt cgatctgctt gggcgagacc tggccaagcc cctccggcag
     5401 cgcgatcgcg tatctgaact gcccgggcac cgcggccgga ctttccttcg cccaaacctc
     5461 cgcccgaacc ttcgtggatt ggttgcggcc cacgcgctgc tgcttcgtga ccggtgcaaa
     5521 tcggatctcc gcgcagccgg tcttcgggtc gttccacttc gtttcggcct ggagcatatt
     5581 cggaccgccg tacgccatga ccgaggccaa aagcgtctgc gagagatcga tatccctggg
     5641 gtccgcgtcc tcgaagttcc acgcttcggc cgtctgctgc acgatgttcg tgggcaacac
     5701 cggcaggtta cgatcgggcg tgaatcggaa taccgcgcgc gcacggcgca tcgtcgtggg
     5761 cagtccactc ccgccgcgca tgatcaccac gaccgcctcc acatcaatgt attgcagctt
     5821 cccgtcatcg cccacgtggc ctttcagcgt ggcatggacc cgctgtccca tgtggacggt
     5881 ccgctggccg ccgtcggttg cttgaaagaa
//
LOCUS       DASTQY010000272         3176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1643986, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000272 DASTQY010000000
VERSION     DASTQY010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643986"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VFR82_08975"
     CDS             <1..416
                     /locus_tag="VFR82_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEU4501770.1"
                     /translation="SNLTIAQIARAYKSKGIGSIVVGDENYGEGSSREHAAMSPRFLN
                     VRAVITKSFARIHETNLKKQGILPLTFADPKDYDKIEMNDRLSVVDLANLAPGKPVTV
                     IIHKASGDVKIQANHSMTAQQITWFKAGSALNALN"
     gene            443..2671
                     /locus_tag="VFR82_08980"
                     /pseudo
     CDS             443..2671
                     /locus_tag="VFR82_08980"
                     /inference="COORDINATES: protein motif:HMM:NF015904.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
     gene            2703..>3176
                     /locus_tag="VFR82_08985"
     CDS             2703..>3176
                     /locus_tag="VFR82_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU4501771.1"
                     /translation="MATESTDTQNIIDQPEVLKPRMVTIEIAGKRVEVPEGITVIKAM
                     WYAGQEVVRGVGCLGGFCGACATYYRTKDDPKVRTCLACQTAVQDGMSFTMMPPFPAR
                     KATYDIQKLQDPKQDLFNLYPETPLCRNCNACTEACPQQIDVREGVWKAVFGDFKS"
BASE COUNT          776 a          878 c          949 g          573 t
ORIGIN      
        1 aatcgaacct cacgatcgcg cagattgccc gtgcgtacaa atccaaagga atcgggtcga
       61 tcgttgttgg tgacgaaaat tacggcgagg gcagcagccg ggaacatgcc gcgatgtcgc
      121 cgcgcttttt gaacgtccgc gcggtcatca cgaaaagttt cgcgcgcatt cacgagacca
      181 atttgaagaa gcagggcatt ctcccgttga ctttcgccga tcccaaggac tacgacaaga
      241 tcgagatgaa cgaccggctg agcgtcgttg atctggccaa cctggcgccg gggaagccgg
      301 tcacggtcat tattcacaag gcgagcggtg atgtgaagat tcaagccaac cacagcatga
      361 ccgcacagca aatcacctgg ttcaaggccg gttcagcgtt gaacgcgctg aactgaaacc
      421 agcgactaaa gagggacata tcatggcaaa agcagacaag atcatctata cgaagaccga
      481 cgaagcgccg atgctggcga cctattcctt tctcccgatt atcaatgcct tttcgaaggc
      541 ggcgggtgtg accgtggaat tgcgtgacat ctcactcgcc ggccgcgtcc tcgccgtctt
      601 tcctgactat ttgacgccgg agcagaagca gcctgatgcg ctggcagagc tcggggaact
      661 ggccaagacg cccgaagcca acattatcaa gctgccgaac atcagcgcct cgatgccgca
      721 gctggtggcc acgatcaagg aattgcagag ccaggggtac aagctaccgg actacccgga
      781 gaatccaaag gacgataagg aaaaagagat caaggcccgc tatgacaagg tgaagggcag
      841 cgcggtcaat cccgtgctcc gcgaaggcaa ctccgatcgc cgcgccccgc tttccgtgaa
      901 ggctcatacc cggaaacatc cgcacaagat gggagcttgg acctccgatt ccaagaccca
      961 tgtttcgcac atgacgggcg gcgatttccg gtcgaacgag aagtccgtga caatcccggc
     1021 agcgaccacg gccaagatcg agttcgtggg agcagatggc aaggtcaccc tgttgaaaga
     1081 caaagttgcc ttgcaggccg gtgaagttct cgacgccaca ttcatgagcg tgaaggcgct
     1141 gcgcaagttc atcgaggagc agatcgagga tgccaaaaag aagggcgtcc tgttttctct
     1201 gcacatgaag gccacgatga tgaaggtctc cgatccgaag atcttcggcc atgcggtaac
     1261 cgtcttctac aaggacgtat ttgaaaagca tggtgagacg ttcaagaagc tcggcgtgga
     1321 tccggacaac gggatcggcg acctgtatgc gaagatcaag tcactgcctg aagagcagcg
     1381 caaggcgatc gaggccgaca ttcaggaggt gtacaagaag cggccaccga tggccatggt
     1441 caactcggac aagggcatta cgaatctgca cgtgccgagc gacgtcatca tcgacgcatc
     1501 gatgccgccg gtcatccgcg actctggcaa aatgtggaat ccggaaggca agctgcagga
     1561 cgtgaaatgc gtgattccag actccagtta tgcgcccgtc taccatgaag tgatcgagtt
     1621 ttgtaagaag catggcgcct tcgatccccg cacaatgggc agcgtgccca acgtcggctt
     1681 gatggcccag gcagcggaag aatacggctc ccacgacaag accttcaagg cgcccggcaa
     1741 cggaaccatc cggatcgtcg atgcctcagg caagacgttg ctggagcaca aggtggaaga
     1801 aggcgatatc tggcgcgcct gtcaggtcaa agatactccg attcaggact gggtcaaact
     1861 ggcggtcacc cgcgccaagg cgaccggcgc tcccgccgtc ttctggctga acaaggaccg
     1921 ggcgcacgat gccgagttga tcaagaaagt gaatgcctat ctcccgaagc atgatacggg
     1981 cggactcgaa atcaagatca tgtctccggc tgatgcctgc cgcttttccc tggagcggat
     2041 gaaggacggc aaggacacaa tttcctgcac cggtaacgtg cttcgtgact acttgaccga
     2101 cttgttcccg attttggaaa tcggcacgag cgccaagatg ctctcaatcg ttccgcttct
     2161 caacggcggc ggcttgtttg aaacgggcgc gggtggttcg gcgccgaagc acgttcagca
     2221 gttccaagag gagggctatc tgcgatggga ctcgctgggc gagttcctgg cgctcgcggc
     2281 ctcgctcgag cacctggcga aggtgggaaa caaccaggtg gccaagatcc tcgccgatac
     2341 gctggaccaa gccaacgcaa agttcctcga gagcaacaag tcgccggctc gtaaagtagg
     2401 tgagatcgac aaccggggca gccattttta cctggcgctg tactgggcgt aagctttggc
     2461 cgcacagacg gccgataaaa agatcgcaga gaggtttacg aagatcgcca aagacctgag
     2521 cgacaatgtt gcaaagatcg atcaagagtt gctggcggcg caaggcaagc ctcaggatgt
     2581 cggcgggtac tatcatccga acgatgagaa ggcggccaag gccatgcgtc ctagtgccac
     2641 gttgaatcgg atcattgacg caattgcata acactcgccg agcggcgggg ctgtgagagt
     2701 gtatggcaac agaaagcacc gatacgcaaa acatcatcga ccaacccgag gtgttgaagc
     2761 cccgcatggt gacgatcgag atcgccggga agagggtgga agtgccggag ggcatcaccg
     2821 tcatcaaggc catgtggtat gccggtcagg aggtcgtgcg gggtgtcggc tgtctcggcg
     2881 gcttctgcgg ggcctgtgcg acctactatc ggacgaaaga cgatccgaag gtgcgcacct
     2941 gtctcgcttg tcagacggca gtgcaggacg gcatgtcatt caccatgatg ccgccgttcc
     3001 ccgcccgcaa agccacgtac gacattcaaa aacttcagga ccccaaacaa gatctcttca
     3061 atctctatcc tgagacgccg ctctgccgga attgcaatgc ctgcacggaa gcctgcccgc
     3121 agcagatcga cgtgcgggag ggggtatgga aggcggtgtt cggcgatttt aagagc
//
LOCUS       DASTQY010000273         4035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1641968, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000273 DASTQY010000000
VERSION     DASTQY010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641968"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="VFR82_08990"
     CDS             <1..1298
                     /locus_tag="VFR82_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501772.1"
                     /translation="DTDLLAGILKDLAAAGNTVVVVEHDRQMIEAADHIVELGPQSGE
                     KGGEVICAAPAGEFLRDRRAITARYLRGEEHIPLPASRRSGTGKVLVIAGASEHNLKD
                     LVVRFPLGMLICVTGVSGSGKSTLVEDTLYAALARAFRTKTLPMGRFKAIRGIEHLKG
                     VRLIDQQPIGRTPRSNPVTYLKAFDEIRRLFSGEREALRQGFTPGHFSFNAEGGRCER
                     CEGAGVEKLEMYFFEDIYAPCERCDGRRFKPEILAIRYRGKTISDVLALTVDEARSFF
                     SGTPKLQERLHLLSSIGLGYLRLGQPATTLSGGEAQRLKIAAELKDASAKDLLYIMDE
                     PTTGLHMDDVKKLLATLQKLVNTGNTLVVVEHNLDVIKSADWVIDLGPEGGQAGGQIV
                     AEGRPEQVVKCDASYTGRCLAQAMAEAADRLILEPHPIA"
     gene            complement(1270..2646)
                     /locus_tag="VFR82_08995"
     CDS             complement(1270..2646)
                     /locus_tag="VFR82_08995"
                     /inference="COORDINATES: protein motif:HMM:NF023330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 family protein"
                     /protein_id="HEU4501773.1"
                     /translation="MDSSRAQSGADALIKKLHERPHIPLADWLRAPAHVHYKAFRMAD
                     PPAERKASREEFQSCLTAFGVPKDETGFHETFGYGVKQAGNGERLIIIWQAHTEYYNY
                     QIWHLLDGAQKDIAFGPLTFPDYKFPISPLGTEVCRLDILLSSYSLPPREHMRSMMPG
                     PVLYGSKILDEETSLVTSFTHDEFERERYWVALGPTRAQASRLKEIVDAIVRIETYYH
                     LLLMQKPLFSAAIDQVYKFEQVHLKQREIISGHIAHADPQTLQRWLNSLTQDLLKTNR
                     IAGKLHFELSASVPYDKIVHTTLASLNEKALDTYRPVSDYVLSGVTGVAEGYQQLLKR
                     IDTLRAGFEGIISIIRARVDLILEAQNLALLQSVDKTTKSQVLLQHTVEGLSVIVIAY
                     YVAGLGGYIFKGLQEIGWLSNANLTSAIFVPVAIGLAFGITTFSKKFLHRKLSREQPP
                     TQSGAAQE"
     gene            complement(2673..3929)
                     /locus_tag="VFR82_09000"
     CDS             complement(2673..3929)
                     /locus_tag="VFR82_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEU4501774.1"
                     /translation="MLQPPEEACFRFTRSCIFILALIAPTLFFGCGVAYTIVKSESKL
                     DNLSVPMTKGQVIDEIGRPDRVLRDDGRMLVWEYSLTARRQWLYELGLCPISVWFGGC
                     IIYPFTNVAMEHEREYPHHVVLVNEELCAWGTPSAILQRRRTCESTGVQRGWQPGAVG
                     KPEPVVTGLGPINRDTVDRYQTMAVMLLEDAPGVAGSGSRVAGIVTTLLLDLDINMVE
                     RAKLDEVLKEQVIQLTHADDANVLKVGKLVGAQAIIVGGVQEWERHEQSRMNKVSLSF
                     RMIDVETGQLLFNGEGHLTDFTTDDPESSARLIVHRILARFGSQTGLLGSGRIGVNWE
                     LLEEKGARFYMVRELRSGLPAERAGLKVGDHIVACNGSSLSGVRSDREAKHLCQVQAG
                     ATLQLDVSRANSPVRLSVTAEKRPGL"
BASE COUNT          856 a         1161 c         1165 g          853 t
ORIGIN      
        1 gcgatacgga tttgctggcg gggattctca aagaccttgc cgcagccggc aacacggtgg
       61 tcgtcgtgga acatgaccga cagatgatcg aagcggccga ccatattgtc gagctggggc
      121 cacaatccgg cgagaagggc ggtgaagtca tctgcgccgc gccggctggc gaatttctgc
      181 gcgatcgccg tgcgattacc gcccgctatc tcaggggcga agagcacatt cccctccccg
      241 cctccaggcg atccgggact ggcaaagtcc ttgtgatcgc cggcgcgagc gaacataacc
      301 tgaaagacct ggtcgtgcgg tttcccctgg gcatgttgat ctgcgtcaca ggggtctccg
      361 gctccggcaa gagcacgctc gtggaagata cgctgtatgc cgcattggcg cgcgcgtttc
      421 ggacgaaaac tctgccgatg gggcgcttca aggcgatcag ggggatcgag catttgaaag
      481 gtgtacgatt gatcgaccaa caaccgattg gccgaacacc ccgctccaat ccggtgacct
      541 acctgaaagc cttcgacgaa atcaggcgcc ttttttcggg agagcgtgag gcgttacggc
      601 agggtttcac gcctggacat ttttctttca acgccgaagg cggccggtgc gagcgctgtg
      661 aaggagccgg cgtcgagaaa ttggaaatgt attttttcga ggatatttat gcgccgtgcg
      721 agcgctgcga cggcagacgg ttcaaaccgg agattttggc cattcggtat cggggcaaga
      781 cgatttcaga cgtgctggca ttgacagtcg acgaggcgcg ctcgtttttt tccggaacgc
      841 cgaagctgca ggagcggctc catcttctgt cgtccatcgg cctgggctat ctgcggctcg
      901 gccaaccggc gacgacgctg tccggcggcg aagcccagcg gttgaagatc gcggccgagt
      961 taaaagatgc cagcgcaaag gatctcctct atatcatgga tgaacccacg accggtctgc
     1021 acatggatga cgtaaaaaag ctgctcgcca cactgcaaaa gttggtgaat acaggcaata
     1081 cgctcgtcgt ggttgaacac aacctggatg tgatcaagtc ggctgactgg gtcatcgacc
     1141 tggggccgga aggaggccag gcaggcggtc aaatcgtcgc tgaaggcaga cctgagcagg
     1201 tggtaaagtg cgatgcgtca tacacagggc gctgcttggc tcaagccatg gcggaagccg
     1261 ctgaccgact tattcttgag ccgcacccga ttgcgtagga ggctgctcgc gcgacagctt
     1321 cctgtgtaaa aacttcttgc tgaacgtcgt gatgccgaag gccaagccga tggcgaccgg
     1381 aacaaaaatc gccgaggtca gattggcgtt gctcagccat cctatttctt gcagaccctt
     1441 aaaaatatag ccaccgagac cggcgacata ataagcaatc acgatgaccg acaatccttc
     1501 caccgtatgt tgcaacaata cctggctctt cgtggtttta tcgacgctct gcagcagcgc
     1561 caaattctgt gcttccagaa tcaaatcgac gcgggcccgg atgatcgaaa tgatgccttc
     1621 aaaaccggct cgcagcgtgt cgatgcgctt gagcaactgt tgatagcctt cagcaacacc
     1681 cgtcacgccg ctcagcacgt aatcggagac aggtcgatag gtgtccagcg ccttttcgtt
     1741 caatgacgcc agagttgtat gcacaatctt gtcgtacggc accgaggctg acagctcgaa
     1801 gtgcagcttc cccgcaatcc ggttcgtctt cagcaagtcc tgggtgaggc tattgagcca
     1861 gcgttggagc gtctgcgggt cagcgtgcgc gatgtgcccg ctgataatct cgcgttgttt
     1921 caaatggacc tgctcaaact tataaacctg atcgatcgcg gccgagaaca gcggcttttg
     1981 catgagcagc agatggtaat aggtctcaat gcggacgatc gcgtcgacga tttctttcaa
     2041 gcgcgaggcc tgtgcgcggg tcgggccgag ggccacccaa tagcgctctc gttcaaattc
     2101 gtcatgggta aaactggtga ccaggctggt ctcttcatcg agaattttac tcccatacag
     2161 cacggggcct ggcatcatcg atcgcatgtg ttctcgcggc ggcagcgaat aggatgatag
     2221 caggatgtcg agccggcaaa cttccgtacc gagcggcgag atgggaaatt tataatccgg
     2281 gaaggtcaac ggcccgaatg cgatgtcttt ttgcgcaccg tcgagcaaat gccagatctg
     2341 atagttgtag tattccgtat gcgcctgcca gatgataatc aatcgttcgc cgttgccggc
     2401 ttgtttgact ccatacccga acgtctcgtg aaacccggtc tcatccttcg ggacgccgaa
     2461 cgccgttaag caggattgaa actcctctct gcttgccttt cgttccgccg gcggatcggc
     2521 catgcgaaaa gccttgtagt ggacgtgtgc gggcgcacgg agccagtcgg ccagcgggat
     2581 gtgcggccgc tcatgcaatt tcttgatcaa cgcatcggca cccgactggg ctcgcgagct
     2641 gtccatgcga ttcctctcct accctcctgg ggttacaatc cgggtcgctt ctcagccgtg
     2701 accgacagcc gcactggact gtttgcccgg ctgacgtcca actgcaatgt ggccccggct
     2761 tgtacctgac agagatgttt ggcttcacgg tcgcttctga ctccggacag cgaagatccg
     2821 ttgcaagcca caatgtggtc gccgactttg agtccggccc gttcagcggg aagcccgctc
     2881 cggagctccc gtaccatgta aaaccgcgct cccttctcct ccagtaattc ccaattgacg
     2941 ccgatacgtc cggatcccag caagccggtc tgggaaccaa aacgggcgag aatacgatgg
     3001 acgatgaggc gggccgaact ctcgggatcg tcggtggtaa aatctgtcag gtggccttcg
     3061 ccattgaaca gcaactgccc tgtctcaacg tcgatcattc ggaacgaaag ggacactttg
     3121 ttcatgcggc tctgctcatg acgttcccat tcttgcacgc cgcctacaat gatggcctgt
     3181 gcccccacga gtttgccaac cttgagcacg tttgcatcgt ccgcgtgggt cagttgaatg
     3241 acctgttctt tcaacacttc gtcgagtttt gcccgctcca ccatgttgat gtcgagatcg
     3301 agcaacaacg tcgtgacgat gcccgccacg cgggagcctg atcctgctac gcccggcgca
     3361 tcttccaaca acatcactgc catggtctga tagcggtcga cggtgtctcg attgattggg
     3421 ccgagccctg tcacgacagg ctcgggcttc cccacagcac ccggctgcca gccgcgctgc
     3481 acaccggtcg attcgcaggt tcttcggcgc tgaaggatcg ctgagggcgt gccccaggcg
     3541 catagctctt cattcacgag cacaacatga tgcggatact ccctctcatg ttccatggcg
     3601 acattggtaa atggatagat gatgcatccg ccgaaccaga ctgaaatcgg acagagaccg
     3661 agttcgtaga gccattgccg gcgggccgtc aaagaatatt cccagaccag catgcggccg
     3721 tcatcacgca gcacccgatc cggccggccg atttcgtcga tcacctgccc cttcgtcatt
     3781 gggacggaca ggttgtcgag cttcgattca gatttgacga tggtgtaagc gacaccgcag
     3841 ccgaagaaca gagtgggtgc gatcagcgca agaatgaaga tgcaggagcg ggtgaaccga
     3901 aagcacgcct cttccggtgg ctgcaacatc gcctggagag taacactcca tacgcaccgc
     3961 cgcaatggat gcatcccgcg acgctagcct gatgagtttg gagaccgcgc ttgcgatgaa
     4021 ccgctggtgt cgatt
//
LOCUS       DASTQY010000274         3865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_329402, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000274 DASTQY010000000
VERSION     DASTQY010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329402"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFR82_09005"
     CDS             <1..345
                     /locus_tag="VFR82_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501775.1"
                     /translation="TQQLTLNIKVKRHDKFAALFMDLEGAIDRLNVHELINRIREAAE
                     KAKMDIIVNFEHLKLAAPDALKALADSEAIKAAVPNVKVRYRKFKDAFETSMQELSLS
                     GVEMLTEDMQDA"
     gene            338..1600
                     /gene="lysA"
                     /locus_tag="VFR82_09010"
     CDS             338..1600
                     /gene="lysA"
                     /locus_tag="VFR82_09010"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU4501776.1"
                     /translation="MHNFEYRQGELYCEQVPVSQIAKEIGTPCYIYSHATLTRHIRAY
                     DSAFKSIPHLIAFAMKANSNLAILRLMAKEGSGVDIVSGGELFRAIKAGVPPSKIVFA
                     GVGKNAEEIRDALKAEILMFNIESSAEMHAINDVAASIGRKARVALRINPDIDPKTHP
                     YISTGLKKSKFGIAADRALEEFRIASSLRHIEVVGVHAHIGSQLTEVTPFVESLKKVI
                     ALIEALKGQGTNIRYLNIGGGLGITYSDEKPPLPQELADAVSPLVRNLDLTLVMEPGR
                     VIVGNAGILVTKTLYEKIGESKRFIIVDAAMNDLIRPSLYNAYHDIRPVSEALLHRPK
                     HAVDVVGPVCESGDFLAKDRTFPEVKPGDLLAVMSAGAYGFVMASNYNSRPRVPEVLV
                     KDGEVHVIRTRESYDDLVKGESIPAFLN"
     gene            1637..2509
                     /gene="dapA"
                     /locus_tag="VFR82_09015"
     CDS             1637..2509
                     /gene="dapA"
                     /locus_tag="VFR82_09015"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU4501777.1"
                     /translation="MFTGSLVAIVTPFRKGKVDERAFGDLIEWQIANGTNGIVPCGTT
                     GESATLTHEEHHRVVRLTVEAVRRRVPVIAGSGSNSTDEAISLTRHAKEAGADGALLI
                     TPYYNKPTQEGLYRHYKAVAEAVDLPQILYNIPGRTGVNMLPATIARLAAMKNIVGVK
                     EGSGSVQQASEIAQTCGERITVLAGDDALTLPMMAVGGKGVITVTANVVPRDMAQLVA
                     TFVAGRVDEARQIHFRLSPLFAALFYETNPIPVKEALGMMGRIDPELRLPLCPMGNDN
                     RNQLARVMKEMRLI"
     gene            2539..3342
                     /gene="dapB"
                     /locus_tag="VFR82_09020"
     CDS             2539..3342
                     /gene="dapB"
                     /locus_tag="VFR82_09020"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU4501778.1"
                     /translation="MIKVIVAGAAGRMGSRLIALIKDSTALTLAGAIESKGHHALGED
                     AGEAAGSGKTGMPITDDLAGLLEKGEVVIDFSVPEATLSHLRAVAQQRRAMVIGTTGF
                     NAAELEELRSLARQVPCVFSPNMSVGVNLLYKVIGEMAKTLGDDYDIEIIEAHHRLKK
                     DAPSGTALKIAEVLARAVNRDLNQVGVYARKGLLGERKKQEIGIQTIRAGDIVGDHTV
                     LFGGMGERIEVTHRASSRDTFARGALRAARWVVKQPPGLYDMMDVLNLK"
BASE COUNT          907 a         1035 c         1141 g          782 t
ORIGIN      
        1 actcaacagt tgacgctcaa catcaaagtc aagcggcacg acaagtttgc ggctctgttt
       61 atggatctcg agggcgcaat cgaccggttg aacgtccatg aactcatcaa tagaatcagg
      121 gaagcggccg aaaaagccaa aatggacatc atcgtcaact tcgagcattt gaagctcgcg
      181 gcgccggatg cgctcaaggc actcgccgat tccgaagcga ttaaagctgc tgtgccgaac
      241 gtgaaagtcc gatatcggaa gtttaaggat gccttcgaaa cctcgatgca ggagttgtca
      301 ctgtcgggcg tcgaaatgct gaccgaggat atgcaggatg cataatttcg aatatcgcca
      361 gggtgagctg tattgtgagc aggtgccggt cagccagatc gccaaagaaa tcggcacgcc
      421 ctgttacatc tatagccacg cgacattgac ccggcatatc cgtgcgtatg acagtgcgtt
      481 caagagcatt ccccacctca ttgcgtttgc catgaaggcc aattccaatc tcgccattct
      541 gcgtctaatg gcgaaggaag gcagcggagt cgacatcgta tcgggcggcg aattgttccg
      601 cgccatcaaa gcgggtgtac caccgtcaaa aattgtcttt gccggggtcg gaaaaaatgc
      661 ggaagaaatc cgtgatgcgc tcaaggccga gattttgatg ttcaacatcg aatcctccgc
      721 cgaaatgcac gccatcaatg acgtagccgc ctcgatcggc cggaaggccc gcgtggcctt
      781 gcgaatcaat ccagacatcg acccgaaaac gcatccctac atttccaccg gcctcaagaa
      841 aagcaagttc ggcatagcgg ccgatcgtgc cctggaggaa tttcggatcg cctcctccct
      901 ccggcacatt gaggtggtgg gcgtccatgc gcacatcgga tcgcaactga ccgaagtcac
      961 ccccttcgtg gaatctttga aaaaggtgat tgcgctgatc gaagcgctca agggtcaggg
     1021 gaccaacatc cggtacctca acatcggcgg cggattgggg attacctatt cggacgaaaa
     1081 gccacccttg ccgcaagagt tggccgatgc cgtgtcgcct ctcgtccgga acttggatct
     1141 gaccctggtc atggagcccg gccgtgtcat cgtcggcaac gctgggatcc tggtgaccaa
     1201 gacgttgtat gaaaaaatcg gagaatcgaa gcgcttcatc atcgttgatg cagccatgaa
     1261 cgaccttatt cgcccgagcc tctataatgc ctatcacgac atccgtccgg tttccgaagc
     1321 gttactgcac cgccccaagc acgcggtcga tgtcgtgggg ccggtgtgcg aatcgggtga
     1381 ttttctcgca aaagaccgca cgtttccgga agtcaagccg ggagatctgc tggccgtcat
     1441 gagcgcaggc gcctatggct ttgtcatggc ctccaactac aattcccggc cccgcgttcc
     1501 ggaggtgctg gtgaaagatg gtgaagtgca tgtcatccgc acccgggaaa gttatgacga
     1561 tctggtcaaa ggggaaagca ttccagcgtt cttgaactga ccgcggtgtg agtcaacctt
     1621 cgtctgccga ggaatgatgt ttactggttc actcgtagcg attgtgacgc cgttccgcaa
     1681 gggtaaggtc gacgagcgag cattcggcga tctcattgag tggcaaatcg caaacggcac
     1741 aaacggcatc gtgccttgcg ggacgaccgg cgagtcggcc acattgacgc acgaggaaca
     1801 tcaccgcgtc gtgaggctca cggtcgaagc cgtcaggcgc cgggtccccg tgattgccgg
     1861 cagcggttcc aacagcaccg atgaagccat ttcgctgacc cgccacgcga aagaagcagg
     1921 ggcggatgga gcactgctca tcactcctta ttacaacaag ccgacacagg aagggctata
     1981 ccgtcactat aaggcggtgg cagaggccgt cgatcttccc cagatcctct acaacatacc
     2041 cggcaggaca ggcgtcaata tgctgcctgc cacaatcgcg cgcctggcgg ccatgaagaa
     2101 tattgtggga gtgaaggagg ggagcgggtc ggtgcagcag gcgtccgaga ttgcccagac
     2161 ctgcggcgag cgcatcaccg tgctcgcagg ggacgatgcg ttgacgttgc ccatgatggc
     2221 cgttggaggc aaaggtgtga tcacggtcac cgccaatgtc gtgcccaggg acatggccca
     2281 gctcgtggca acgttcgtgg ccggccgagt ggatgaggct cgccagattc atttccgctt
     2341 atcgcctctt tttgcggcgt tattctacga aaccaatcca atccccgtga aggaagcgct
     2401 gggaatgatg ggcagaattg atcctgaact gcgattaccc ctgtgcccga tgggaaacga
     2461 caaccgcaat cagttagccc gcgtcatgaa agaaatgcgg ctgatctagt cattgcacag
     2521 ctcaagaatt cacgtctcat gatcaaagtc attgtagcgg gagccgccgg ccgaatggga
     2581 agccgtttaa tcgcgttgat caaggattcg actgccttga cgttggccgg cgcgattgaa
     2641 agcaagggac atcatgccct tggcgaagat gccggagaag cggccggttc aggaaagacc
     2701 ggcatgccta tcaccgacga tctcgccggt ctactggaaa agggtgaagt cgtcatcgat
     2761 ttttccgtcc ccgaggcgac cctcagccat cttcgagcag tggctcaaca acgccgagcg
     2821 atggtcattg gcacgaccgg tttcaatgcc gccgaattag aagagttgag atcgttggcg
     2881 cggcaggtgc cttgcgtctt ctcgcccaac atgagcgtcg gagtcaatct tctttataag
     2941 gtgattggag aaatggcgaa aactctggga gatgattacg acattgagat cattgaggcg
     3001 caccatcggc ttaaaaagga tgcgccgagc gggaccgccc tgaagattgc ggaagtgctg
     3061 gctcgggcgg ttaatcgaga cctcaatcag gtgggcgtct atgcacgcaa agggctgctg
     3121 ggagaacgga agaagcagga gatcgggatt cagacgatac gcgccggcga catcgttgga
     3181 gatcatacgg tgctctttgg tggaatgggc gagaggattg aagtcacgca ccgggcgagc
     3241 agccgagaca ccttcgcacg cggggcattg cgtgcggctc gttgggtggt gaagcaaccc
     3301 ccgggcctgt atgacatgat ggacgtgctg aatctcaaat gacgagatca gcgagcagtc
     3361 tcgctaggca ggtatgtaat gaatatggga gtaggggaga agcgagagaa gaggacgtgc
     3421 tgacagagtg aacagtcgca agccgtagca cgaccctggc ggctcatgct cgtcagacgg
     3481 ggtcggaaaa attgttcatc gtgtcaatgt ctggccgtaa gaggacgagg ttcttgatcc
     3541 gctgccttcc gacgaccaca gttcaagcag gcaaatacca tgatcgcaag cccggcatcc
     3601 aaatccactg cccgttccgg caacatcgtt ccgcgacact tgtcacaggt cttcatagcg
     3661 gtcactctaa cactgtgacc gaggagtgcc tatccatcat aagtactggg gcttcggggg
     3721 aatttaggcc tggtagatgg ggtagaagct aggccattcg agctatacgg ttcgaggccg
     3781 accgttggtg aaggttcaca tcgggcgttg acagcatctc aacgctccca taggattgtg
     3841 gcagccgacc tgacctctac tatgt
//
LOCUS       DASTQY010000275         5063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1430866, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000275 DASTQY010000000
VERSION     DASTQY010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430866"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="VFR82_09025"
     CDS             <1..1408
                     /locus_tag="VFR82_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU4501779.1"
                     /translation="RIQAAEDISFQAMRQKLTEIVEIIRSDQAVESVTAFSGGGTNNV
                     GRSFVALKPLQERKVTTDEVVTRLRPKLAKVPGASTYLQTIQDLRIGGRASSAQYQYT
                     LQSVDLAELNTWAPRVEQQLRTLPEIADVNSDQQDKGLQSLVVFDRSTASRLGISPQL
                     IDDTLYDAFGQRLVSVMYRPLNQYHVVMEAAPQYWQNPATLHDIYVRSPSGAQVPLSA
                     VARYEPTSTLLAVNHQGQFPAVTLSFNMVPGISLGQAVAAIERELQQMALPAGIRGSF
                     QGTARAFRASSDNQVWLILAALVTVYIVLGILYESYIHPITILSTLPSAGVGALLALL
                     LFKTELSVIAMVAIILLIGIVKKNAILMIDFALEAERKEGKGPAEAIYEACLLRFRPI
                     MMTTMAALFGALPLALGTGVGSELRRPLGIAIVGGLLVSQLLTLYTTPVVYLYLDRMR
                     LRFLRRMPGSIEPSPLPH"
     gene            1503..2057
                     /locus_tag="VFR82_09030"
     CDS             1503..2057
                     /locus_tag="VFR82_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501780.1"
                     /translation="MMSRLSTSQRISTVFLAVTVLTGCPRVLYLDYHPSASITGSGPI
                     RVDTFIYAGHPTGLMKQKEVESGNYDPERLFLSQDIGEFFANALRKELTSAGYEARPD
                     STRAISGTIEQFFLDYVGGQEQRFQIQTLFQVSRDGGPSFMTSCRTERQQAKDWMKSG
                     SLIEHGVRDCIEAFVKEAQAAGAL"
     gene            2092..3276
                     /locus_tag="VFR82_09035"
     CDS             2092..3276
                     /locus_tag="VFR82_09035"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU4501781.1"
                     /translation="MPGFEYIGEEEHRQVLEVMASGVLMRYGFDGPRKGQWKCRELEQ
                     KLAMRFGARRAHVCSSGTSALSTALAVCGVGAGDEVILPPFTFVADLETVLLAGAVPV
                     FAEIDETLCLDPRSVAAAMTPRTKAVLVVHMCGAMARIDAIMDLCRTHHVTLIEDAAQ
                     AAGATFHGKALGTFGRVGCLSFDYVKTITCGEGGAVLTDDEEAYRLAQAYTDHGHDHV
                     GADRGADGHPHIGANFRLSELHAAIGVAQLARLDDILKVQRRHKAMLKDGLAGIRGLQ
                     FRELPDPAGDSATFLSLLLPSEHDARQAAKALAEASVDGCFYWYDNNWHYHRKWDHIK
                     AFTSPGRLAIDRFGYGSDLTKITLLQSDAVIGRTISMLIKLRWTEEQVRDRLARMKSV
                     LA"
     gene            3308..4066
                     /gene="kdsB"
                     /locus_tag="VFR82_09040"
     CDS             3308..4066
                     /gene="kdsB"
                     /locus_tag="VFR82_09040"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEU4501782.1"
                     /translation="MNVAIIVPARIGSTRFPKKLLHRIKDKPVIVWTADRIKKEAPDL
                     PLYFAVDHESLADVLRGGGYEAIMTDPAHACGTDRIAEANRRLKAKYVINVQADEPLV
                     TGGQIRQLVELIQSDTEMATLGTPVRYDKDYRNPNHVKLVCDEKGYAVYFSRAPIPYL
                     RDTHGAFDAEVAAGIPVLIHLGLYAYTASFLELFCTLAPGKLEQVEKLEMLRAMERGH
                     RIKVGVTKETLVEIDTPEQAVEFEQVVTERFPAH"
     gene            4107..>5063
                     /locus_tag="VFR82_09045"
     CDS             4107..>5063
                     /locus_tag="VFR82_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU4501783.1"
                     /translation="MYRNLRLVPQLIYGRGSINQLGDILTKQRTDAGSPVVFLIDAVH
                     EKRAFIQKLPLRANDLVILVDVGTEPKTQYVDELTERIKAFSNRQPDAIVGIGGGSAM
                     DLAKAVSIMLTNPGSAADYQGWDLVKYPAIYHLGIPTLAGSGAEVSRTAVLTGPIKKL
                     GINSDFTPFDQIVMDPDLIATVPQNQRFFTAMDCYIHSCESLQGTFINEFSRAFAESA
                     RDICRDVFLSHCEDVVADERLMVASYFGGLSIAYSQVGVCHALSYGLSYVLGVRHGVG
                     NCIVFNHLEEYYGQDVREFQQMIQQNRIDLPSGVTNGIDEASM"
BASE COUNT         1081 a         1477 c         1445 g         1060 t
ORIGIN      
        1 caggattcag gccgcggaag acatttcttt tcaggcgatg cgacagaagc tcaccgagat
       61 tgtggagatt atccgaagtg accaggccgt ggagtccgtg accgcgttca gcggcggagg
      121 cactaataac gtcggccgct cgtttgtcgc cctgaaaccc ctgcaggagc gcaaagttac
      181 cacggacgag gtggtaacga gactccgtcc caagctcgcc aaggtcccgg gcgcttcgac
      241 atatctgcag accatccagg atttgagaat cggaggccga gcgagcagcg cacaatatca
      301 atacacactt cagagcgttg atctcgctga actcaacacg tgggcaccac gcgtcgagca
      361 acaacttcgc acactcccag agatcgccga cgtcaacagc gaccaacagg acaagggcct
      421 gcaatccctg gtggtgttcg atcgcagcac cgcctcccgg ctcggcatca gcccccagct
      481 cattgacgac acgctgtacg acgcgtttgg tcagcgactg gtttcagtca tgtacagacc
      541 cttgaaccaa taccatgtcg tgatggaagc cgcccctcaa tactggcaga acccggcaac
      601 gctccacgat atctatgtgc gctcgccgag cggtgcccaa gtccctttga gcgcggtggc
      661 gcgctatgaa cccacctcga cattgctggc ggtgaaccat caggggcagt ttccagccgt
      721 cacgctttcg ttcaatatgg tgccgggaat ctccctgggt caggcggtag cggcgattga
      781 gcgcgagctt cagcagatgg ctctgccggc cggcattcga ggcagctttc aggggacagc
      841 cagagcgttc cgggcttcgt cggacaacca ggtatggttg atcctggcgg ccctcgtcac
      901 ggtctatatc gtgctcggga ttctgtacga gagctacata catccaatca caatcctctc
      961 cacgctgccg tccgcgggtg tgggcgcgct cctggcgctg ctcctgttta agacggaatt
     1021 gagcgtgatc gcgatggtcg ccatcattct gctgattggc atcgtgaaga agaacgcgat
     1081 tctgatgatt gacttcgcgt tggaggcgga gcggaaagaa gggaagggac cagcggaagc
     1141 gatttatgaa gcctgcctcc tgcgcttccg gccgatcatg atgacgacga tggccgcgct
     1201 ttttggggcc ttgccgctgg cgcttggaac cggcgtcggc tccgaactac ggcgtccgct
     1261 cggcattgcc attgtcggcg gcctcttggt aagccagctt ctgacactct atacgacccc
     1321 cgtggtgtat ctctatctgg accgcatgcg cctgcgtttc ctcagacgga tgcccggatc
     1381 aatcgaaccc agtccacttc cgcattagac cgttcgcgct gacgctgccc tgttctcacc
     1441 ggctccattt acgctattga cagtcgtcct cactctccgt aggctgccca gagcctgaac
     1501 gcatgatgtc ccggctttcg acttcccaaa ggatttcgac tgtttttctg gccgtcacgg
     1561 ttctgaccgg ctgcccgcgg gtcttgtact tggattatca tccatccgct tcgatcacgg
     1621 gaagcggacc aatccgtgtt gataccttca tctatgcagg tcatcccacg gggctgatga
     1681 agcaaaagga agttgaatcc ggtaactatg atcccgaacg gttgttcctt tcgcaggaca
     1741 tcggcgaatt tttcgccaat gcgctccgga aggagttgac gtcggcaggc tacgaggcca
     1801 ggcccgactc gacccgagcc atctcaggta ccatcgaaca gttcttcctc gattatgtcg
     1861 gcggtcagga gcagcggttc caaatacaga cgcttttcca ggtgtcgcgc gacggcggcc
     1921 cctcgttcat gacatcctgc cgaacggagc gacaacaagc caaagactgg atgaaaagcg
     1981 gttcactcat cgaacacggc gtccgcgact gtattgaagc gttcgtcaaa gaggcgcaag
     2041 ctgcaggagc gctgtagttt cctgcgacga gcatggcgtc gcgagggttc aatgccagga
     2101 tttgaataca tcggcgaaga agaacatcgg caagtgctcg aggtcatggc atcgggcgtc
     2161 ttgatgcgct acggatttga tgggcctcga aaagggcaat ggaaatgccg tgagctcgaa
     2221 caaaagttgg caatgcgttt cggagcccgg cgtgcgcatg tgtgttccag tggtacctcc
     2281 gctctcagta cggcattggc cgtgtgtgga gtcggtgcag gcgatgaggt catccttcct
     2341 ccctttacgt tcgttgccga tcttgagaca gtgctgctgg ccggcgcggt gcccgtcttc
     2401 gcggaaatcg acgagacgct ctgtctcgat ccccgttcag tcgcggcggc gatgacaccg
     2461 agaacgaaag ccgtgctggt cgtgcacatg tgtggggcaa tggccaggat tgatgcgatc
     2521 atggaccttt gccgaacaca ccacgtgacc ctcattgagg acgcagcaca ggcagccggc
     2581 gcaacctttc atggaaaagc gttaggaact ttcggcaggg tgggctgctt atctttcgac
     2641 tatgtgaaaa cgatcacctg cggggaaggt ggagcagtcc tcaccgacga cgaggaggcc
     2701 taccgcttgg cacaggccta taccgaccat ggacatgatc atgtgggtgc cgatcgaggt
     2761 gccgatggac acccgcacat cggcgcgaac tttcggctca gcgaactgca tgcagcaatt
     2821 ggtgtggcgc aactggcacg actcgacgat atcctgaagg tacaacgtcg tcacaaagcc
     2881 atgctgaagg acggattagc tggcattcgc ggtctgcagt tccgagaact gcccgacccg
     2941 gctggagatt cagccacctt tctgagtctg ctgttgccat ctgagcacga tgctcgacaa
     3001 gcggccaaag cacttgcaga ggccagcgtc gacggctgct tctactggta cgacaacaat
     3061 tggcactacc accggaaatg ggaccatatc aaagcattca catcgccggg ccgtctggcc
     3121 atcgaccgct tcggatacgg cagcgatctc accaagatca ccctgctgca atccgacgca
     3181 gttatcggtc ggacgatctc catgctgatt aaactgcggt ggaccgagga gcaagtgcgt
     3241 gatagactcg cccggatgaa atccgtgttg gcctgaactc tgcccgttcg tccaacttga
     3301 gccgcccatg aacgtcgcaa tcatcgtccc ggcccgaatc ggctccacgc ggtttccgaa
     3361 gaaactactt caccggatca aggacaaacc ggtgatcgtt tggaccgccg accgcatcaa
     3421 gaaagaagcg cccgacctcc cgctctactt tgcggtggat cacgagtctt tggcagacgt
     3481 gctccgcggg ggcggttatg aggcgatcat gacggatccg gcccacgcct gcggcaccga
     3541 ccggattgcc gaggccaatc gtcgcctcaa ggccaagtat gtcatcaacg tacaggccga
     3601 cgaaccgctg gtgaccggtg ggcagattcg tcagctggtc gagcttattc aatctgatac
     3661 ggagatggcg acgttgggaa cccccgttcg atacgacaag gattaccgca atccgaacca
     3721 cgttaagctg gtctgtgatg agaagggcta tgcggtatat ttttcgcgtg cacccatccc
     3781 gtatctacgc gatacacacg gcgcattcga cgccgaggtg gcggccggca ttccagtcct
     3841 cattcatctc gggctctatg cctacacagc atcatttttg gaactattct gcacgctagc
     3901 gcccggcaaa ctggaacagg tcgagaagct cgagatgctg cgggcaatgg aacgcggcca
     3961 ccgtatcaag gtcggagtca ccaaggaaac gttggtcgag atcgatacgc ccgagcaggc
     4021 cgtggaattc gaacaggtgg tgaccgagcg atttcccgct cattagccaa acgctggcga
     4081 cttcgacaac cggagaaggg acgatcatgt atcgtaatct gcgcctggtc ccccagctca
     4141 tctacggacg aggcagcatt aatcagctcg gcgacatcct gaccaagcaa cgcaccgacg
     4201 ccggatcgcc cgtcgtcttc ctgatcgatg cggtgcacga aaaacgagcg ttcattcaga
     4261 aactgccgtt acgcgccaac gacctggtca tcctcgtaga cgtcggaacg gagcctaaaa
     4321 cccaatacgt ggatgagctc accgaacgaa tcaaggcgtt ttccaaccgg cagccggatg
     4381 cgattgtcgg gatcggaggc ggcagtgcca tggatctggc caaggccgtc tccatcatgc
     4441 tgaccaatcc aggttcggct gcggattatc agggttggga cctggtgaag tatcccgcga
     4501 tctatcatct cggcattccg accttggccg gaagtggtgc ggaggtgtcc cgcacggctg
     4561 tccttaccgg ccccatcaag aagctcggta ttaattctga ttttacccct ttcgatcaaa
     4621 tcgtcatgga cccggatttg atcgcgaccg tcccgcaaaa tcagcggttc ttcaccgcaa
     4681 tggattgcta cattcactcg tgcgaatcgc tgcaaggcac cttcatcaac gaatttagcc
     4741 gggcctttgc cgaaagtgcc cgggacatct gccgggatgt atttctcagc cactgtgagg
     4801 acgtcgtcgc cgacgaacgc ttgatggtgg cctcctattt tggaggcctc agcatcgcct
     4861 actcacaggt cggcgtctgc catgcgctgt cctacggact gtcctatgtt ttgggcgtgc
     4921 gtcatggcgt aggcaattgc atcgtcttca accatcttga agaatattat gggcaggatg
     4981 tacgagagtt tcagcagatg atccaacaga accggatcga tctgccgtcc ggggtgacca
     5041 atggaatcga cgaagccagc atg
//
LOCUS       DASTQY010000276         6815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1028024, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000276 DASTQY010000000
VERSION     DASTQY010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028024"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VFR82_09050"
     CDS             <1..1142
                     /locus_tag="VFR82_09050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU4501784.1"
                     /translation="ALLLWSLGCQPEEPASKAQRSPLQADTQLVEVQVTKPTRRDLAY
                     TVKLPANIAPLYQTTLYAKVSGYLKWIGPDKGDHVKKGQVVSIIDAPEVEEQYLQAAA
                     DYKIKKITFERLKNVWKESPDVIAKQDVDVAEAAYEGARTLMQQRAVLRDYTKVRAPY
                     DGTVTARFADPGALIQVATSSATSAIPLFTIMDLSTVRIYANVSQDDSPWVTPGKTHA
                     TVTVPELPDRSFSGTITRSTLALDPSTRSLLVEIDLPNPDHLLRPGTFAEMAIALREI
                     PNALVVPPQAVSSGGKGTSLFVVEQGKAKSVPVQTGITDGKWIEITSGLKGDEEVVAV
                     GKRQLLDGSPVRASPFQLPDAKFSQQRYERRSPGVQEAETPAVPR"
     gene            1173..2546
                     /locus_tag="VFR82_09055"
     CDS             1173..2546
                     /locus_tag="VFR82_09055"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU4501785.1"
                     /translation="MSDILRRKSLFILLITLLIPSVTEAQPPGRTSEPPPRGSFLGLQ
                     QAIELALEKHPLLQEAAATLKAATARTEQARAIYYPHVDANFDTAAGAGRLNPRFLVG
                     GFLVQPNLSQYVGGVSVSQRIFDFGFSQNTVGSVQLAERAQEIDINARQALAVLNVQA
                     SYLTSLKHRRLVQIAEETVRERGIIKGQIEALYRQQLKSKLDVDLVQVELTNAESLLV
                     KARNGLKSSFANLNRAMGVMGAEDYVLEDLPIEVRPQRPLDMLISESLSHPEFRKTKE
                     LTASAEAKITAAKRQYLPTVSAYASGGDFQVYDSSRSQANQTGGWWAAGGFVSMPLFT
                     GFMIENQVREAKAQKAAAEAASMNIEQALTQQVTNSYLDTISFAQQIKLGEEQVKTSQ
                     EALNLAKQRYKLGLGSIVEVTQAEVALTAAQTRLAEAQYDYKIAEVTLAYASAGAAQL
                     EVDPTIR"
     gene            complement(2571..3317)
                     /locus_tag="VFR82_09060"
     CDS             complement(2571..3317)
                     /locus_tag="VFR82_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501786.1"
                     /translation="MSRKSGTERTWRQLVGLGLLSAFHIPIPALLLFAGCAIPHVPYR
                     TIYEDPVNYVRLEEDDAVLPEWPPGHHAHPKTITTGEMRVLLKGMTVQEHRIWLQKWL
                     QGDAPLVPAFKDEETELLAPQLTEAFASANYNERVTFYLSLPQTSVKRVITSGGMYVR
                     GDELHVILGNWQIVYGIPTYGMIYDRRYPMRPTAAKGFDLFFQPSDAVVTQRSSVWDT
                     LFANTKDELVIDMVRWQGAVTPYSALTHLP"
     gene            complement(3335..3844)
                     /locus_tag="VFR82_09065"
     CDS             complement(3335..3844)
                     /locus_tag="VFR82_09065"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU4501787.1"
                     /translation="MAELTDKTRATISVTSKGQAVGDIVVRFFHDVAPGHVKNFIDLA
                     TKGFYNGTTFHRVIPKFMIQGGDPNSKNADRSSHGMGGPGYKIKAEFNSKPHKRGVVS
                     MARANDPDSAGSQFFICVADSHFLDWQYTAFGEVVSGMDVADTVVNMKRDGRDNPLER
                     VEMTVSISE"
     gene            complement(3926..4954)
                     /locus_tag="VFR82_09070"
     CDS             complement(3926..4954)
                     /locus_tag="VFR82_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501788.1"
                     /translation="MLMNRSLLLLGMTLLTVSLVGSAFAQQLSSSPFESQAASIQALT
                     VVGSDILYAGSFGLGVFRSDDRGATWTKSSEGVSDPFILSLATAKDGTVYVGTFRGGV
                     FRTTDQGKTWVPVNAGLKRKEVKTLLAVGDLLYAGTADGAYRLNLSDDRWGVVTSGLD
                     EILVHALAQSADGTLYAGTSGKGVLKFKANTTGWVRLHDGLKDHEGMVENFIRVLTID
                     REGGIYAGTFDGGVFRSPDGGSTWRPISRALPNDSIRGIIFNSRGLFVATGHGIFKTT
                     DKGRQWVPLNNGLTSMAVQVLVESSSGSLYAGTNAGAFRSDDDGKSWSPINEGLEGGL
                     APPPFLFR"
     gene            complement(4964..5737)
                     /gene="tatC"
                     /locus_tag="VFR82_09075"
     CDS             complement(4964..5737)
                     /gene="tatC"
                     /locus_tag="VFR82_09075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU4501789.1"
                     /translation="MPPMTNPLAAHIQTIKRRLLIIAATLLVFLMVAFTFSAEMVDWL
                     NRPFPNPLVFYGPTEALFASIKVSFLAAVLASLPVVFYQFWKFVQPALLPKEQRWGIP
                     IIALAGVLFALGLLFCNLVILPLVIDFFVSFGMDRDITPELSVGTYIDFNVKFLLTFG
                     CAFELPLVLSILARVGVVTAEQLAKYRKHAILSALIISAVVTPDATLFTMMLMAVPLM
                     VLYEIGILGARLFGRNHPTDMNLPLDPDLPVGTAGHRVR"
     gene            complement(5774..6013)
                     /locus_tag="VFR82_09080"
     CDS             complement(5774..6013)
                     /locus_tag="VFR82_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEU4501790.1"
                     /translation="MLTDNVIVERLRQSNTEFRELEESHHRLDLELNELQKRHVLTPT
                     EELTKKQMQKEKLAKKDKMAELIRTYRDHEHATAH"
     gene            complement(6038..6559)
                     /gene="rimI"
                     /locus_tag="VFR82_09085"
     CDS             complement(6038..6559)
                     /gene="rimI"
                     /locus_tag="VFR82_09085"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU4501791.1"
                     /translation="MMPPDWTIEAASLESLSEILAVEEACFSAPWTRKMLEGELSGNP
                     FAHFLVARPRRAEEASKQAILGYLCYWIVFEEVRLMNLAVIESMRHRGIARSLVSTAL
                     QTGVSQSAHRAVLEVRASNRAAQTLYQGFGFSQTAVRPKYYTNPVEDAVLMELDPIFV
                     GGAGHTGGSTPPR"
     gene            complement(6556..>6815)
                     /locus_tag="VFR82_09090"
     CDS             complement(6556..>6815)
                     /locus_tag="VFR82_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501792.1"
                     /translation="RSVRIIEAEEHMRPSAVSIGMAGIARLRKGERAGVGISPLYVQR
                     PEAEIQYEESGEISPVSRRQQKLARKLSRSPTAATRTRRQT"
BASE COUNT         1534 a         1994 c         1881 g         1406 t
ORIGIN      
        1 tggctcttct gctatggtcc ctcggatgcc agcccgagga accagcaagc aaggctcagc
       61 gatcccccct ccaggccgat acccaactag tcgaggtaca agtcacgaag ccgactagga
      121 gggatctcgc ctacacggtg aagcttccgg cgaacatcgc tccgctgtat cagaccaccc
      181 tgtatgcgaa ggtgtcgggc tacttgaaat ggatcggtcc ggataaaggt gaccatgtca
      241 agaaaggcca agtcgtgtcg attatcgacg cgccagaagt cgaggagcaa tatctccagg
      301 ctgccgcaga ttacaagatc aagaagatca cgttcgaacg actgaagaat gtctggaagg
      361 agtctccgga tgtcattgct aaacaggatg tggatgtggc tgaagcggcg tatgaaggcg
      421 ccaggactct catgcaacag cgggcggtct tgcgagacta cacgaaggtg cgggccccct
      481 acgacggcac ggtcactgcc cgttttgccg atcccggggc cctgatccaa gtcgccacgt
      541 cttcggccac gagcgccata cccttgttta cgattatgga tctcagcaca gttcgcatct
      601 atgccaacgt gtcgcaggac gattccccgt gggtgacgcc cggcaagacc cacgccaccg
      661 tgacggtacc tgaacttccc gaccgttcat tcagcggaac catcactcga tcgaccttgg
      721 cgctggatcc ttcgacacgc agtctcttag tcgaaatcga cctgccgaat cccgaccatc
      781 ttctccggcc cggaacattc gccgagatgg caatcgcctt gcgagaaatc cccaatgcgc
      841 tcgtcgtgcc gccacaagcc gtcagcagtg gcggcaaggg tacgtcgctc ttcgttgtcg
      901 agcaaggcaa agccaagtcg gtgccggtcc aaacaggaat caccgacggc aagtggatcg
      961 agattacaag cggactcaag ggggacgaag aagtcgtcgc tgtcggaaaa cgtcaactgc
     1021 tggatggaag ccccgtgcgc gcatcccctt tccaacttcc cgacgcaaag ttctctcaac
     1081 aaagatacga gcgacgttca cccggagtcc aggaagcgga gacgccggcg gttccacgtt
     1141 aaccctcaca tgttgcacgt aggaggagcc ttatgtctga catccttcga cgaaaatcgc
     1201 tctttatatt gctgatcacg ctgctgatcc cttcggtcac ggaagcgcaa ccgccaggac
     1261 gcacctccga accaccgccc cggggcagtt ttttaggtct gcagcaagcc attgaactag
     1321 cgctcgagaa gcatccactc ttgcaggaag ccgccgccac cctgaaagcg gccaccgcta
     1381 gaacagaaca ggcccgagcc atttactacc cgcatgtgga tgcgaacttc gacaccgcgg
     1441 caggcgccgg acgactgaat ccccgctttc ttgttggcgg attcctggtt caacccaacc
     1501 tcagtcaata tgtcggaggc gtgtcggtca gccagcggat tttcgacttc gggttcagcc
     1561 agaacaccgt gggttcggta caactggcgg aacgggctca ggaaattgac atcaatgccc
     1621 gccaagcgct cgcagtgttg aacgtacagg cctcctatct cacaagtttg aaacaccggc
     1681 ggttggtaca gatcgcagaa gaaacagtcc gcgagcgagg catcatcaag ggccagatcg
     1741 aagcgctcta tcgccagcag ttgaaatcga agctcgatgt ggatttggtg caggtcgaac
     1801 tgacgaacgc cgaatcgctt ctcgtcaagg ctcgcaacgg actcaaatcc agttttgcca
     1861 atttgaaccg cgccatgggt gtgatgggag cagaggatta tgtccttgag gacctgccta
     1921 tcgaggtccg tccccagcgt cctctcgaca tgctcatcag cgagagcctg tcacaccccg
     1981 aatttcgcaa gacaaaagaa ctgacggcat cggccgaagc aaaaattacg gccgccaaac
     2041 gtcaatacct cccgactgtc tccgcctatg caagcggagg cgatttccag gtctacgata
     2101 gcagccgttc ccaggccaac caaacaggag gctggtgggc agcgggggga ttcgtatcca
     2161 tgccgctctt taccggtttc atgatcgaga atcaagttcg cgaagccaag gcacagaagg
     2221 cggctgccga agccgccagc atgaacattg aacaggcgtt gacgcaacaa gtgaccaatt
     2281 cgtatctcga caccatctca ttcgctcaac agattaaact gggggaagag caggtcaaaa
     2341 cgtcacaaga ggcattgaat ctggccaagc aacggtacaa gttgggtctt ggatcgatcg
     2401 tagaggtgac gcaagcggaa gtcgcgctga ccgccgcaca gacgagattg gctgaggcgc
     2461 agtacgacta caagatcgcg gaggtgacgc ttgcctatgc ctcggcaggc gccgcacaac
     2521 tggaggtcga tccgactata cgatagacac gcatcctcgg tggatcatcg ctatgggaga
     2581 tgcgtgaggg cggaataggg cgtgactgct ccctgccagc ggaccatgtc gatgacaagc
     2641 tcgtcctttg tattggcgaa gagtgtgtcc cagacgctac tacgctgcgt caccactgca
     2701 tctgacggct gaaaaaacag gtcaaaaccc ttcgccgccg tggggcgcat cgggtagcga
     2761 cggtcgtaaa tcatgccata cgtcggaatc ccatacacaa tttgccaatt accgagaatc
     2821 acatgcaact cgtcgccacg cacgtacatg cctcccgagg tgatcacgcg cttgaccgag
     2881 gtttgaggca gactcaggta aaacgtcacc cgctcattgt agttggcgct agcaaaggcc
     2941 tcggtcagct ggggcgccag aagctccgtc tcttcgtctt tgaatgccgg caccaaaggt
     3001 gcatcacctt gcagccattt ttgaagccaa atgcgatgct cttgaacggt catccctttg
     3061 agcagcaccc tcatttcccc cgtggtgatc gtcttgggat gcgcatggtg cccaggaggc
     3121 cattcaggga gtaccgcatc atcttcttcc aatcggacgt aattgaccgg gtcctcatag
     3181 atggttcggt agggcacgtg tggaatcgcg cagccggcga aaaggagtag cgctggaatg
     3241 ggaatgtgga aggccgacag taacccaagc cctaccagct gccgccacgt gcgttccgtt
     3301 ccggacttgc gactcatcgc tcggcactca tacgtcattc actgatgctg actgtcatct
     3361 cgacgcgctc gagggggttg tcgcggccat cacgcttcat gttcacgacc gtatcggcca
     3421 cgtccatgcc gctcacaact tcgccgaagg cggtatattg ccaatccagg aaatgtgaat
     3481 cggccacgca aatgaaaaac tgcgacccgg cgctgtcagg atcgttggca cgtgccatcg
     3541 agaccactcc tcgcttgtgg ggcttgctgt tgaattcggc tttgatctta tatcctggtc
     3601 cacccatgcc gtgggaggat cggtcggcat tcttgctgtt agggtcgccc ccctgaatca
     3661 tgaacttggg gatgacacga tggaacgtcg tgccgttgta aaaacctttt gtggctaagt
     3721 cgatgaaatt cttgacgtgg cctggtgcga cgtcatgaaa gaacctcacc actatatccc
     3781 cgaccgcttg tcccttgctg gtaacagaaa tggtggccct ggttttgtca gtcaactcag
     3841 ccatctcgcc gttcctttct tgttggctat gatcaatcgc cctgcttttg ccgttgagaa
     3901 tgccggcgcc ttccgcttca ggaaactacc gaaataagaa aggcggcgga gccaggcctc
     3961 cctctagccc ttcgttgatc gggctccagc tcttgccatc gtcgtcgctc cggaatgccc
     4021 cggcgttcgt ccccgcatac aatgatccgg aagaagactc gaccagcacc tgcaccgcca
     4081 tgctggtgag tccgttgttc agcggtaccc actgccggcc cttgtcggtg gttttgaaaa
     4141 tgccgtgccc tgtcgcaacg aacaggccac gactgttgaa aatgattccg cgtatggaat
     4201 cgttcggcaa ggcgcggctg atggggcgcc atgtactgcc accgtccggg cttcggaata
     4261 cgccgccgtc aaacgtccct gcatatatcc cgccttcgcg gtcgatggta aggacccgaa
     4321 taaaattctc caccatacct tcgtgatcct tcagcccatc atgaaggcgc acccagccag
     4381 tcgtgttcgc tttgaatttc aggactccct tcccggacgt cccagcatag agcgtgccgt
     4441 cggctgattg tgccaaggca tgaaccagaa tttcatcgag gccgctcgtc acgacccccc
     4501 aacgatcgtc gctcagattc aagcgatagg caccgtcagc ggtccccgca tacagcagat
     4561 cacccaccgc caacaacgtc ttcacttcct tccgcttcaa tcctgcgttg actggaaccc
     4621 aggtcttgcc ttgatcggtg gttcggaaca ctcctcctcg aaaggtcccc acatacacag
     4681 ttccgtcttt tgccgttgcg agagataata tgaacgggtc actcacccct tcactggatt
     4741 tcgtccaggt cgctccgcga tcgtcgcttc gaaagacgcc gagcccgaac gagccggcat
     4801 acaggatatc cgaccccacc acagtgagcg cctgaatact ggccgcttgc gattcgaagg
     4861 ggctcgatga cagttgctga gcgaacgccg aaccgaccag cgacacggta agcagggtca
     4921 tgccaagaag aagcaatgat cggttcatca gcatgagcgt atcttaccga acccgatggc
     4981 ccgctgtccc aactggtagg tcagggtcca gtggaaggtt catgtccgta gggtggttgc
     5041 ggccgaacag acgggcgccg aggatgccga tttcatagag caccatcaag ggcacggcca
     5101 tgagcatcat ggtgaaaagc gtggcatcag gagtcaccac agcggagatg atcaaggcgc
     5161 tcaagatagc gtgcttgcga tatttagcca actgctccgc cgtgacaacg ccgacacgag
     5221 cgagaatgct caacacgagg ggcaattcga atgcacatcc aaaagtcagc agaaatttga
     5281 cgttaaaatc aatgtacgtg ccgacgctca gctctggggt aatatcccgg tccatgccga
     5341 agctgacgaa aaagtcgatg acgaggggaa ggatgacgag gttgcaaaac agaaggccca
     5401 gggcaaatag cacccccgcg agcgcgataa tggggatgcc ccaacgctgc tccttcggga
     5461 gcagcgctgg ttggacgaac ttccagaact gataaaaaac gacaggcagg ctagccagca
     5521 ccgctgccag gaaagagact ttgatcgagg cgaacaaggc ttccgtcggg ccgtaaaaga
     5581 ccagcgggtt gggaaacggc cgattcaacc agtccaccat ctcggctgaa aacgtaaagg
     5641 ccaccatgag gaacacgagc agggtggctg cgataatgag caggcgccgt ttgatggtct
     5701 gtatgtgggc ggccagggga ttggtcatcg gcggcatgta cggactttcg gcttggactc
     5761 acggccacac ccctcaatgt gccgtagcgt gttcgtgatc gcgatacgtg cggatgagtt
     5821 cggccatttt atctttcttg gcaagttttt ctttctgcat ctgcttcttg gttaattctt
     5881 ctgtcggcgt caagacatga cgtttctgca attcgttcaa ctccagatcg aggcgatggt
     5941 gggactcttc cagttcgcgg aattctgtgt tggattggcg aagccgctcc acaatcacat
     6001 tgtccgtcag cattgtcgta cctcctggtt gagtgaatta tcgcggtggt gtcgatcctc
     6061 ccgtatggcc ggcacccccc acgaatatgg ggtccaactc catgagcacc gcatcttcca
     6121 cgggattggt gtagtacttc ggccggacgg ccgtttggga aaacccgaat ccttgataca
     6181 acgtttgggc ggcacggttg gaggctcgta cctcaaggac ggcccgatgc gcggattgcg
     6241 acacgcctgt ctgtagcgcg gtagaaacta gggatctggc gatcccccga tgccgcatcg
     6301 actcgatgac cgccaggttc atcagccgga cttcctcgaa gacgatccag taacagagat
     6361 agccgagaat cgcctgtttc gacgcttcct ccgcgcgcct cggccttgca accagaaagt
     6421 gggcgaaagg atttccgctc aactcccctt ccagcatttt gcgcgtccag ggggccgaga
     6481 agcaagcttc ttctactgct aaaatttccg aaagagattc aaggcttgcg gcctcaatcg
     6541 tccagtcagg gggcatcatg tctggcgcct ggttctggtc gccgctgtgg gcgagcgact
     6601 gagtttccgt gccagttttt gttgccgtct tgacacgggt gagatctcac cggactcctc
     6661 atactgaatc tcagcctctg ggcgttggac gtacaaaggg ctgatgccca caccggcgcg
     6721 ctcacccttt ctgagccgag cgatgccggc catcccgatg gagaccgccg agggtcgcat
     6781 gtgctcctca gcttcgataa tcctgactga acgcc
//
LOCUS       DASTQY010000277         8765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1612355, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000277 DASTQY010000000
VERSION     DASTQY010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8765
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612355"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /gene="lnt"
                     /locus_tag="VFR82_09095"
     CDS             <1..1281
                     /gene="lnt"
                     /locus_tag="VFR82_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEU4501793.1"
                     /translation="RMLVPRYGLFAAPCLWVALELLRTYLLTGLPWSLLGYSQYRQLD
                     VIQIADHLGVYGVSFLIVLANVALAELYLWLMPLFRGFRPARLPWELVTTSAMLVGLS
                     WAYSTSLIVSDGMEQLKASLRVGVVQPNIDQAVKWDQAYRDETLRRYDRLTESFGYGV
                     DLGVWPEAATPFIYEREPVYQLQLVSMASRASAPLLFGSPAVRFDADRKPFLLNSAYL
                     LSPQGQLLGRYDKQHLVPFGEYIPLKSSLLFFLDKLVEGIGDFQAGPGPIVLSFQPSE
                     DSGFSRSVKFGVVICYEVIFPDLVRRMALGGAEFLVTITNDAWFGDSSAPFQHFAMVV
                     FRSVENHLAFARAANTGISGFIDPFGRIIEASPLFTEAALQATIPVRQTQTFYSRHGD
                     VFAYGCMLISLLLSLYGVFGTKGAEPDAIAATPV"
     gene            1299..2442
                     /gene="prfB"
                     /locus_tag="VFR82_09100"
     CDS             join(1299..1367,1369..2442)
                     /gene="prfB"
                     /locus_tag="VFR82_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_155970262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU4501794.1"
                     /translation="MLDEIRVRVRAVDEQVSELRGHLDLSRMTAQLEELEAKMGQADF
                     WNDARAAAGVSRHKVRLERELQQWRDIEAKLSDVTALLELAQESGDAGLERELASELD
                     QFEPRLAALRVEHLLSGELDQNNAIVAIHPGAGGTESQDWAQMLLRMYVRWAEGKGFK
                     VDTLDLQAGEEAGIKSATVSVAGPYAYGYLKAEAGVHRLVRISPFDANKRRHTSFASV
                     FVYPELNDDIEVAIEEKDLRIDTFRAGGAGGQNVNKVETAIRITHLPTGIVVQCQNER
                     SQLQNRNGAMKILKARLYEQEQKKKEAEFNAIVGEKKDIAWGNQIRSYVFQPYQMVKD
                     HRTGHEVGQVSAVMDGDVDGFIEAYLKQKLIRGSDQLSPAHGSDDV"
     gene            2472..3953
                     /gene="lysS"
                     /locus_tag="VFR82_09105"
     CDS             2472..3953
                     /gene="lysS"
                     /locus_tag="VFR82_09105"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU4501795.1"
                     /translation="MDEANEQRQQRIKKLDQLRAAGVAPYGTRFEAKDRAGEIIKLHG
                     QKTKEALESEKIACTIAGRIVALRRFGKAAFAVLQDGSDRLQTYLKKDLLSERAYTVT
                     EQLDLGDWIGVTGTLFRTKTNEFTVEVHDLSFLSKALRPLPEKWHGLTDVETRYRQRY
                     VDLIANPEVHDIFVARSRIITAIRAFLTQRGFLEVETPMMHPIPGGAAAKPFVTHHNA
                     LGVDLYLRIAPELYLKRLIVGGFPRVFEINRNFRNEGISTIHNPEFTMLEFYVAYADY
                     QDLIRLTEELISSLTQQLFGKTVIEYQGQVINLTPPWRRWSYREAILEVNRLAPAVLT
                     NREQAVAAAKELHVPVDPKWPLFNIVNEIFEETVEPNLQQPTFITDYPIEISPLARRK
                     DADPSLTDRFELYIAGREIANAFSELNDPLDQRERFEGQAAQRAAGDEEAHVVDEDFL
                     RALEYGMPPTAGEGIGIDRLAMLFTNQASIRDVVLFPQLRPEK"
     gene            3958..5235
                     /locus_tag="VFR82_09110"
     CDS             3958..5235
                     /locus_tag="VFR82_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEU4501796.1"
                     /translation="MALPYEIFVGLRYLRAKRRNRTISLNTFVSVAGITLGVAALIGT
                     VGIMTGFKEDIQAKILGTTAHIIVQDRMKDGMSDYDGVAKQLESVPDVVAVTPFILKQ
                     VMLTTQSSAQGVVLRGIDPGREGYVTALAHNMVSGQLEELSQPIAVKLPPPDNPSGPL
                     QSSDKPGIILGKELALRLGAFVGDTVSVVSPTGTISAMGMVPKIRIFAVAGIFQSGMY
                     EYDSSLAYIDLGEAQKFFSMGETVTGIEIKVTDVFRAGDIARRVEQALGFSYGARDWM
                     QMNRNLFSALKLEKTMMFLLLVLITIVASFNIVSTLTMIVTEKQKEIAILKAMGATKK
                     SIRRIFMLNGLIIGLSGTAIGIPLGYAFLWLIERLWTFDPTVYYISKIPVHVLAEDVF
                     LVAGSAILISFAATVYPSLQAARLEPVAALRYE"
     gene            5281..5943
                     /locus_tag="VFR82_09115"
     CDS             5281..5943
                     /locus_tag="VFR82_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4501797.1"
                     /translation="MITVKDLHKSFPMGEQTLTVLRGIDLTIERGELIAVVGASGAGK
                     STLLHILGLLDRPSRGTVHFEGQDLFQLSEAAQAEFRNRRIGFVFQFHHLLPEFTALE
                     NACMPALIQRREPEEVRQEAIGILKDVGLGPRLQHKPGELSGGEQQRVAVARALLQKP
                     DLVLADEPTGNLDTHTGDALFALMRELNKARGTTFVIVTHNDKLSAQADRILHMQDGQ
                     II"
     gene            6170..7336
                     /gene="traF"
                     /locus_tag="VFR82_09120"
     CDS             6170..7336
                     /gene="traF"
                     /locus_tag="VFR82_09120"
                     /inference="COORDINATES: protein motif:HMM:NF025106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HEU4501798.1"
                     /translation="MRLGLSFGAIVFLVFLLLHGKALGAEFVIVGPRAMGMGGAGVAI
                     TTDALATYWNPAGLAMTQTVDIRIVGSAQAIDRLGIADAVSDLENFDTTDTSPANVAK
                     AQDIANRINRPGANTSFNGAAGLYLKGHFGEHAFGFNISNVPTGGGFVSVPVGVSCSP
                     NPNCAAPGPISSVSLNGAMAVRALEARQAAFSYAYAFADKTFAIGVTAKIIQGAAYNG
                     THSLVGGQEIKLSDSLGEATITTTYGIDVGAIYRPSSWLRFGVVAKDLNQPEFDAPDG
                     TKIKLGPQVRGGMALNPYSSLTLSADVDVTSNKTLVPGVKSQVLSLGAEQTILTEFLS
                     FRLGAFKNMQDAGTPFTPTAGLGVRIFMVRLDVGGGYDFREKGALVSGSLSLTF"
     gene            7372..8013
                     /locus_tag="VFR82_09125"
     CDS             7372..8013
                     /locus_tag="VFR82_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501799.1"
                     /translation="MSIRAQHWVRVASCCLTFMIAGCGGLQEVWEGPGAKQFRPQAIA
                     VLPPMASQYDSAREDIQDVLSGALTKTARIERVVTPESVTDIFQGSKEAFDSLVFYFS
                     RLEMTGQSDKESAIKLGKALNVDSFLVVRVNSWEYIRKEGDNVGRVGLSLRLVDATTG
                     TTVWKARHEKSSSYMFYRPNLKDVANELAKEMIAYMPPQYQNRSSLSQGLASK"
     gene            complement(8039..>8765)
                     /gene="trpA"
                     /locus_tag="VFR82_09130"
     CDS             complement(8039..>8765)
                     /gene="trpA"
                     /locus_tag="VFR82_09130"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU4501800.1"
                     /translation="PGLAETEELVLALEKAGADIIELGVPFSDPIADGPVIQQAAERA
                     LRSGTSLRKILLMVAAIRTKSQVPLVLMAYYNSIHAYGPDRFCQDATAAGVDGLIVPD
                     MPPDEAGPLRGPACASGLHLIFLLAPTSTPQRRAFVARESKGFVYYVSLTGITGAKLA
                     DMADVGANVEKIRELSDTPIAVGFGVATPQDAARLSRYADGVVVGSAIVRQIAAHQNG
                     GMVSEVSHFVHALKTAMVPRMAP"
BASE COUNT         1825 a         2493 c         2536 g         1911 t
ORIGIN      
        1 cggatgctgg tgccgcgata tggcctgttc gccgcaccct gtttgtgggt ggcgctggaa
       61 ttgctgcgca cctatctcct cacggggctt ccctggtcgc ttctcgggta ttcacagtat
      121 cgtcagctcg acgtgattca gatcgcagat cacttgggcg tgtacggcgt gtcctttctg
      181 attgtgctgg ccaatgttgc cctggcggaa ttgtatctgt ggctgatgcc gctctttcgc
      241 ggcttcagac cagcgcggct tccatgggaa ttagtgacga cctcggcgat gctggtcggc
      301 ctgtcctggg cctacagcac ctccttgatc gtcagcgatg ggatggagca gctaaaggcc
      361 tcattgcgag tcggcgtggt gcagccaaac attgaccagg cggtgaaatg ggatcaggcc
      421 taccgggatg agacgcttcg gcgctatgat cgactgacgg aatcgttcgg ctacggcgtc
      481 gatctcgggg tgtggcctga ggcagcgacc cccttcatct acgaacgcga gccggtgtat
      541 cagctgcaac tcgtgtccat ggccagccgt gcttcagccc cgctcctctt tggcagtcct
      601 gcagtgcgtt ttgatgcgga ccgtaaaccg ttcttgctga acagtgccta cctgttatcc
      661 cctcagggcc aactgcttgg ccgttatgac aagcagcatc tggttccctt cggcgagtac
      721 attccattga aatcgtctct gctgttcttt ctcgacaagc tggtggaagg catcggcgat
      781 tttcaggccg gacccggtcc gatcgtgctg tcgttccaac cttccgagga ttcgggcttc
      841 tctcgctcgg tcaaattcgg cgtggtcatc tgttatgaag tcatttttcc cgaccttgtc
      901 cgccggatgg cgcttggcgg tgccgagttt ctcgtgacga ttacgaacga tgcctggttt
      961 ggggattcgt ctgccccctt ccagcatttc gcgatggtcg tgttccggtc ggtcgaaaat
     1021 catctggcct tcgcgcgggc cgcaaacacc ggcatttcgg gttttatcga tccattcggg
     1081 cgcatcatcg aggcctctcc cctttttacc gaagctgccc tgcaggcgac gattccggtt
     1141 cggcaaacgc agacctttta cagccgccat ggagatgtct ttgcctacgg ctgtatgtta
     1201 atctccctgc tgctctcgct gtacggcgtg ttcggcacca aaggagccga accggacgcg
     1261 atcgcggcta ctcccgtcta aaggaaagga tcgttttcat gctggacgaa attcgtgttc
     1321 gcgtacgtgc ggttgatgaa caagtatcgg aacttcgggg gcatctttga cctctcccgc
     1381 atgaccgccc aactggaaga actcgaagcc aaaatgggcc aggctgattt ttggaacgac
     1441 gcgcgcgcgg cggccggcgt gagccgtcat aaagtaaggc tggagcgcga actgcagcaa
     1501 tggcgtgata tcgaggcgaa actcagcgac gtgacggcgc tcctggaatt ggcgcaggaa
     1561 agcggcgatg caggcctgga gcgggaactc gcctctgagc tcgaccaatt cgaaccccgt
     1621 ctggccgcac ttcgggtgga gcatttgctg tcgggcgagc tcgatcaaaa caatgcgatc
     1681 gtcgccatac atccgggcgc gggtggcacg gagtcacagg actgggctca gatgctgctg
     1741 cgcatgtacg tccgctgggc agagggaaaa gggttcaaag tagacacgct ggatttgcag
     1801 gcgggcgaag aagccgggat caagagcgcg acggtgtccg ttgccgggcc ctatgcgtac
     1861 ggttatttga aagcggaagc tggcgtccat cggctcgtac gcatctcgcc gttcgatgcc
     1921 aacaagcggc gtcacacgtc ctttgcctcc gtctttgtct accccgagct caacgacgac
     1981 attgaagtgg cgatcgagga gaaggatctg aggatcgata cgtttcgggc gggcggcgcg
     2041 ggcggccaga atgtgaacaa agtcgagacg gcgatcagga tcacgcattt gccgaccggc
     2101 attgtcgtgc aatgtcagaa cgagcggtcg caactccaga accgcaacgg tgcgatgaaa
     2161 attctgaaag cccgcctgta cgagcaggaa cagaagaaaa aggaagccga gttcaacgcc
     2221 attgttggcg agaaaaaaga tattgcgtgg gggaatcaaa tccgttctta cgtgttccag
     2281 ccctatcaga tggtcaagga ccatcggacc ggccacgagg tcggacaagt ctccgcggtc
     2341 atggacggcg atgttgacgg cttcattgaa gcctatttga agcagaagct catcagaggg
     2401 agcgatcaac tctctccggc tcacggatcc gatgacgtgt agttgcggct agcggtacga
     2461 caggtttgag catggatgaa gcgaacgagc agcggcagca gcggatcaaa aagctcgatc
     2521 agctgcgtgc ggcgggcgtg gccccctacg ggacacgctt cgaggcgaag gatcgggcgg
     2581 gcgagatcat caagctccat ggccaaaaga cgaaagaagc gctggagagt gagaagatcg
     2641 cctgtaccat cgctggacgt attgtcgcgt tgcgtcgatt cggcaaggcg gctttcgccg
     2701 tgctccagga cgggtccgac cgcttgcaga cctatttgaa gaaggatctg ctgtcggaaa
     2761 gggcctacac ggtgaccgaa caattggacc tcggcgattg gatcggggtg acagggacgt
     2821 tgtttcgaac gaagaccaac gagttcactg tcgaggtcca cgacctatcg tttctcagca
     2881 aggcgcttcg tcctctgccg gagaaatggc acggcttgac cgatgtcgaa acccggtacc
     2941 gtcagcgcta tgtcgatctc atcgcaaacc cggaagtgca cgacattttc gtggctagga
     3001 gccgcattat cacggccatc agagcgttct tgactcaacg ggggtttctc gaagtcgaga
     3061 cgccgatgat gcatccgatc cccggtggcg cggccgcgaa gccctttgtc acccatcaca
     3121 atgcgctggg cgttgatctc tatcttcgca tcgcgccgga attatatttg aaacggctta
     3181 tcgtcggcgg ttttccacgt gtctttgaga tcaataggaa cttcaggaac gaagggattt
     3241 cgaccatcca caatcccgaa ttcacgatgc tcgagttcta tgtggcctat gccgactacc
     3301 aggacctcat ccgtctcacc gaggaactga tttcatcgtt gacccaacag ctgttcggta
     3361 agacggtcat agagtaccag ggccaggtga tcaatttgac tcctccttgg cggcgctggt
     3421 cttatcgtga agctatcctg gaagtaaacc ggctggctcc ggccgtgctc acgaatcgtg
     3481 agcaggcggt ggctgcagca aaggaattac acgtgccggt cgatccgaag tggccgctgt
     3541 tcaacattgt gaatgagatt tttgaggaaa ccgtcgagcc gaatctgcag cagccgactt
     3601 tcattaccga ttatccgatc gagatctctc cgctggccag gcgaaaggat gccgacccct
     3661 ctctcaccga tcgattcgag ctctacatcg cgggacggga gattgccaat gcgttttcgg
     3721 agctgaacga cccgctggat caacgggaac gttttgaggg gcaagcggcg cagcgcgccg
     3781 ccggtgacga ggaagcgcac gtagtcgatg aggatttcct tcgggccttg gaatacggca
     3841 tgccgccgac cgcgggcgag ggcatcggca ttgaccggct ggccatgctc ttcaccaatc
     3901 aagcttcgat ccgcgatgtg gtgctctttc cgcagctgcg tccggagaaa taacttcttg
     3961 gcacttcctt acgagatctt cgtcgggctt cggtacttgc gcgccaagcg gcgtaatcga
     4021 accatttcat tgaatacgtt cgtctcggtg gcgggaatca ccttgggcgt cgctgcgtta
     4081 atcggaaccg tcgggatcat gaccggcttt aaagaagata ttcaggccaa aatactcggc
     4141 acgaccgcac acattatcgt tcaggatcgc atgaaggacg gcatgagcga ctacgatggg
     4201 gtcgccaaac agctggaatc cgttcctgat gtcgtggcgg tgacgccgtt catcctcaag
     4261 caggtaatgc tcacgactca aagcagcgct caaggcgtcg tccttcgcgg catcgatccc
     4321 ggacgtgaag gttacgtgac ggcgctcgcc cacaatatgg tgtcgggaca attggaagag
     4381 ctgagtcaac cgattgccgt gaagctgcct ccgcctgaca acccgtctgg tccccttcaa
     4441 tcgtcagaca aaccgggcat cattttgggc aaagagctgg cgcttcgact cggcgccttt
     4501 gtcggagata cggtcagtgt cgtgtcgcca accggcacga tcagtgcgat gggcatggtc
     4561 cctaaaatca gaatcttcgc agtggcaggt atattccagt ccgggatgta tgaatacgac
     4621 tcatccctcg cctatatcga cctcggggaa gcgcagaagt ttttcagcat gggagagacc
     4681 gttacgggca tcgaaattaa agtgaccgat gttttccgag ccggcgacat cgcccgccgt
     4741 gtggagcaag cgctcggctt ttcctatggc gccagggact ggatgcagat gaaccgtaac
     4801 ctgttttccg ccctcaagct cgaaaagacg atgatgttcc ttcttctcgt gctcatcacg
     4861 atcgtcgcct ctttcaatat cgtgagtacg ctgacgatga tcgtcacgga aaaacagaag
     4921 gagattgcca ttctgaaggc catgggtgcg actaaaaaga gtatccgacg gattttcatg
     4981 ctcaacggat tgatcatcgg tctttcgggc acagcgatcg gcattccgct ggggtatgcc
     5041 tttttgtggc tcatcgaacg actatggacc ttcgatccga cggtctacta catctcaaaa
     5101 atcccggtac acgtcttggc cgaggacgtg ttcttggtcg ccggctctgc catcctcatc
     5161 agttttgcag cgaccgtcta tccctcgctg caggccgcaa gactcgaacc ggtcgccgcc
     5221 cttcgctatg aatagcttgc gaagccatat tccgtatcgg cctcgacacg gctcgtgcgc
     5281 atgatcaccg tcaaagacct tcacaaatcg tttcccatgg gcgaacagac cttgacggtg
     5341 ctcagaggga tcgatctcac cattgagcgc ggcgaactca tagccgttgt cggcgcgtcc
     5401 ggcgccggga aaagcactct tctgcacatt ctcgggctgc tcgaccgtcc gagcagaggc
     5461 acggtgcatt tcgagggcca ggatttgttt caactgtccg aagcggcgca agccgaattc
     5521 cgcaacagac gcatcggttt cgtcttccag tttcatcacc tgctgccgga atttaccgcc
     5581 ttggaaaatg cctgcatgcc ggccttgatc cagcgccgtg agcccgagga agtgagacag
     5641 gaggccattg gtatcttgaa ggacgtagga ttgggtcctc gcctgcaaca caagcccggc
     5701 gaattatcag gaggggaaca gcaacgggtg gccgtggccc gtgctctact gcaaaagccc
     5761 gacctcgtgc tggctgatga gccgaccggc aatttagata cccacaccgg agacgccctc
     5821 ttcgctctca tgcgtgagct caataaagct cgcggcacca ccttcgtcat cgtcacccac
     5881 aatgacaagc tctcggctca ggctgatcgg atcctgcaca tgcaagacgg ccagatcatc
     5941 tgacaggctc cttgcgcgca gtgcgaggag ttatcctcgt ctcagacttg ccccttgctt
     6001 ccatacggaa ctgcaactcg gtactacacg gcgacaggat ggtgctcttc gctcgatctc
     6061 atgtctcggc agcgttgtca tcgcttggag atcgctgaga aatccttgac ggccgccggt
     6121 tcatccaata gactcagcga catctgcgtc catacggagc gttgacgcca tgcggcttgg
     6181 actgtcattt ggcgctattg tcttcctcgt cttcttgctt cttcacggca aagcgctcgg
     6241 cgctgaattc gtgatcgtgg gtccgcgtgc catgggaatg ggcggcgctg gcgtcgcaat
     6301 taccaccgat gccttggcga cctattggaa tcccgccggc ctggccatga cacaaacggt
     6361 cgatatccgg attgtgggta gtgcgcaggc gatcgaccgc ctgggcattg ctgacgcggt
     6421 gagtgacctt gaaaactttg atacgactga cacttcaccg gccaacgtag ccaaggcgca
     6481 ggatattgca aaccgcatca atcggcctgg cgccaacacc tcgttcaacg gcgctgcggg
     6541 tctctatttg aaggggcatt tcggcgagca cgcgtttggc ttcaatattt ccaacgtacc
     6601 caccggcggc ggatttgtgt cagttccggt cggcgtcagt tgcagtccca atccgaattg
     6661 cgccgcacca ggacccatct catccgtctc tctcaacggt gccatggcgg ttcgggcgct
     6721 ggaggccagg caagccgcgt tctcctatgc gtatgcgttt gcagacaaaa cgttcgcgat
     6781 cggagtcacg gctaaaatca ttcaaggagc ggcctacaac ggaacccaca gtcttgtggg
     6841 aggacaagag atcaaactct cggatagttt gggtgaggct acgatcacga ccacgtacgg
     6901 catcgacgtg ggtgctatct atcgaccctc atcctggctg cgttttggcg ttgtggcgaa
     6961 ggacctcaac caaccggaat tcgatgcgcc ggacggcaca aaaatcaagc tcggccctca
     7021 ggtccgtggc ggcatggcac tcaaccccta ttcttcgctg accttgtcgg ccgacgtcga
     7081 cgtgacatcg aacaagacgt tggtgccagg ggttaagagt caagtcttga gcttgggagc
     7141 ggagcagacg atcctgacgg aatttctctc attccgcctc ggcgcgttca agaacatgca
     7201 ggatgccggc actcccttca cgccgacggc aggacttggt gtacggatct ttatggtacg
     7261 cctcgatgtg ggaggcggat atgatttccg tgagaaggga gccttggtct ccggttctct
     7321 gtcgctcacc ttctaacccc tatcgtgatt cactaatggt atactgagct catgtccatt
     7381 cgcgcacagc attgggttcg tgtggcgtcc tgttgcctca cgttcatgat cgccggctgc
     7441 ggcggtttgc aggaagtctg ggaaggcccg ggtgccaaac aatttcggcc gcaagccatc
     7501 gccgttctgc ctccaatggc aagccaatat gacagtgccc gtgaggacat acaggatgtc
     7561 ctctcaggag ccctcacgaa aacggccaga atcgagcgtg tcgtgacccc tgaaagcgtg
     7621 accgatattt ttcagggttc aaaagaggca ttcgactccc tggtgttcta tttctcacgc
     7681 ctcgagatga cggggcaatc agacaaggag tcggccatca aactggggaa ggccttgaac
     7741 gtcgattcat tcctagttgt tcgggtcaac tcgtgggagt atatacgcaa agaaggagac
     7801 aacgtgggtc gggttgggct cagtctgcgg ctcgtcgatg caacgaccgg gacaaccgtc
     7861 tggaaggcgc gccacgaaaa gtcgagtagt tatatgttct accgtcctaa tttgaaggat
     7921 gtcgccaacg agctcgccaa agagatgatc gcttacatgc ctccccagta ccaaaaccgc
     7981 tcctcgttgt cacaagggct cgcaagcaag tagatcgtcc cgcacagtcg atcgcgcgct
     8041 acggtgccat acgcggaacc atcgccgtct tgagcgcatg cacgaagtga gagacttccg
     8101 acaccatgcc gccgttctga tgcgcggcaa tctgccgtac gatggcgctt ccaacgacga
     8161 cgccgtcggc ataccgagac agacgcgccg catcctgtgg cgtcgccacc ccgaatccca
     8221 ccgcaatcgg agtatccgac agttcccgga tcttttccac gtttgcgccg acgtccgcca
     8281 tgtctgcgag ctttgcgccg gtaattcccg tcaaggaaac gtagtacaca aagcccttgg
     8341 attcccgcgc gacgaacgcc cggcgttgcg gcgtgctggt cggcgcgagt aaaaagatca
     8401 gatgcaaccc ggaagcgcag gccggtcccc gaagcggacc agcctcatca ggaggcatgt
     8461 ccgggacgat caaaccgtcc actccagccg ccgtcgcgtc ctggcaaaat cggtccggtc
     8521 cgtacgcatg gatgctgttg taataggcca tcagcacaag gggaacctgt gatttggttc
     8581 tgatggccgc gaccatcaaa aggatttttc gcaaggacgt gccgctccgg agcgcccgtt
     8641 ccgctgcctg ttggatgacc ggaccatccg cgatgggatc agaaaacggg acgcccaact
     8701 cgatgatatc agcgccggct ttttccaatg cgagaaccag ctcttcggtt tcggccaacc
     8761 cggga
//
LOCUS       DASTQY010000278         5465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_118195, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000278 DASTQY010000000
VERSION     DASTQY010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118195"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VFR82_09135"
     CDS             complement(<1..962)
                     /locus_tag="VFR82_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU4501801.1"
                     /translation="MKLGGSGLVPYLLLAFGAVIGVVVASDLGWMPSGSAGPDSPANG
                     VVYPVATAPQQPVTGGTAKSFVEIAKTVKPAVVNIAASRSGKAGDGPQSSPFDDPFFR
                     RFFGDEFFKRDQPHRERKERGQGSGVIVDPNGLIITNNHVVNKADDIRVILSDKREFK
                     GKLIGTDTKTDVAIVKIEATGLSTIPLADSDQLEVGEFVLAVGSPFGLTQSVTMGIVS
                     AVGRASMGIAEYEDFIQTDAAINPGNSGGALVNIRGELVGINTAIFSQSGGNMGIGFA
                     VPSNLARSIMDQLVKNGKVVRGWLGVAIQELTPELASQFGLADT"
     gene            complement(972..1895)
                     /locus_tag="VFR82_09140"
     CDS             complement(972..1895)
                     /locus_tag="VFR82_09140"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEU4501802.1"
                     /translation="MADHVQEILSYYTSDNPGTKTTIARLLRSGKLAGTGKLVILPVD
                     QGFEHGPARSFAPNPPGYDPLYHFQLAIDAGCNAYAAPLGFLEAGASQFAGQIPLILK
                     LNNHDVLHDDKDPLPSVTGSVKDALRLGCAAVGYTIYPGSAHCNLMYEQLRAIAEEAK
                     DSGLAVVVWSYPRGSAISKEGETAMDVVAYAAQIAAQLGAHVIKVKLPSAHLEQAAAK
                     KVYESAQISIKTLSERVKHVVQSAFDGRRIVIFSGGAKSEDKNVFEEARAIRDGGGFG
                     SIIGRNSFQRPKAEAVKFLQTIMGIYAGEIQ"
     gene            complement(1928..2941)
                     /gene="fbp"
                     /locus_tag="VFR82_09145"
     CDS             complement(1928..2941)
                     /gene="fbp"
                     /locus_tag="VFR82_09145"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEU4501803.1"
                     /translation="MAHQPLTVEQFLMVNPPASLGGAGSLTNLLSAIGLVGKTLARDL
                     RIAGLLNMLGMTGETNVQGESVKKLDQIANETFVEVLQRDGVAGALASEEMEKPVFVA
                     SPGRPAPYMVLFDPLDGSSNTDVNMPLGSIFSIVAPKKADQLPGESDLLQKGTDQLAA
                     GYFLFGPSTMLVFTTGKGVYGFTLDPNVGEYQLSHPGIKIPSRGKVYAVNEGNYQKWP
                     DGTKRYIDSLKKSDKAVGRPYSSRYSGCLVADVHRLLLGGGIYLYPGEVNKPEGKLRL
                     LYEANPMAMVVEQAGGRASTGTVRILDIEPKKLHQRVPLIIGSTDDVRDAEQYMQGTA
                     VLS"
     gene            complement(3074..3841)
                     /locus_tag="VFR82_09150"
     CDS             complement(3074..3841)
                     /locus_tag="VFR82_09150"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEU4501804.1"
                     /translation="MPAILARCTLTCLLLLVDQMATATGVETAPTQAVQNGERLTYDI
                     SWLNVLAGTAVMEVQPDGAGSRARLVTTAQSRPAISKVFPVDNRVESEFDLSVQLPQH
                     MVFRRREGKNKEDIEYTFNHREGTVTAVRHGTTETLPIPAGTQDLISCLYYVRNQLPM
                     KPGASLTLNVYHDKRNRQVQVRVENIETVEGVWGKAETAQVVVIMPFKGLFINKGDIR
                     VWFTTDERRIPVRMKAKVIIGSIVADLVDGFSSKSSP"
     gene            complement(3874..5274)
                     /locus_tag="VFR82_09155"
     CDS             complement(3874..5274)
                     /locus_tag="VFR82_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEU4501805.1"
                     /translation="MGLFREYDLRGIVGEELTEAIAEQVGRAYCTYVKKRGVQTISVG
                     RDGRLSSPALFKSLVSGLLAGGVNVIDIGICPSPLLYFSLFQLPVQGGIMITGSHNAA
                     EYNGFKVCVGKEAIHGETIVELRRILETGSFTSGQGRLSEHTIIPDYLDYLRTSFKGV
                     NAGRLHVVIDCGNGAAALVAKEALEILGCRVTGLYCDLDGRFPNHHPDPTVLENLDDL
                     VKAVKQHKADVGIGYDGDADRIGAIDEQGRVLWGDRLMVVYARDILRTIPGSTIISEV
                     KASQCLYDDIAKQGGRAIMWKTGHSLIKAKMKSEKAVLAGEMSGHMFFADRYFGYDDA
                     IYASCRLVEILAKTGKPLSTLVADLPATSVTPEIRVDTPDSIKFELVKKVQDRLAGYA
                     KSSRPIGPNNDVVRDVVTIDGVRAIFEDGWGLIRASNTQPALVLRFEATTADRLSAIR
                     ALVEGELAEVKRSMLS"
     gene            complement(5288..5461)
                     /locus_tag="VFR82_09160"
     CDS             complement(5288..5461)
                     /locus_tag="VFR82_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEU4501806.1"
                     /translation="MFELHVGQSTAIPVETKHRLENFGQDTLNIIEVQNGPYLGEDDI
                     VRFKDDYGRTTAR"
BASE COUNT         1140 a         1636 c         1487 g         1202 t
ORIGIN      
        1 ttggtgtctg caagaccgaa ttgcgatgcc aattcgggag tcaattcctg tatggcgact
       61 cccagccagc ctcggacaac cttgccgttt ttgaccagtt gatccatgat ggagcgggcc
      121 aggttgctcg gtacggcaaa accgattccc atgttgccgc cgctttgact gaagatcgcc
      181 gtattgatac cgaccaactc gccgcggatg ttgaccaacg caccgcctga attaccgggg
      241 ttgatcgctg catcggtttg gataaaatct tcatactccg caatgcccat gctcgcgcgt
      301 ccgaccgcgc tgacgattcc catcgtgaca ctctgcgtca ggccaaacgg gctgccgaca
      361 gccaggacga actccccgac ctcgagttga tcggagtctg ccaagggaat ggttgacaag
      421 ccggttgctt cgatcttgac gatcgccaca tcagtcttgg tatcggtccc gatcaactta
      481 cccttaaact cccgtttgtc cgagagaatc acccgaatgt cgtcggcttt gttgacgacg
      541 tgattattgg tgatgatcaa gccgttcgga tcgacaatca cgccggatcc ctgcccgcgc
      601 tccttccgct cgcgatgcgg ctggtcacgc ttaaaaaact cgtcgccgaa aaaccttcgg
      661 aaaaacgggt cgtcgaatgg agagctttgg ggaccgtcgc ctgccttgcc gctccggctg
      721 gcagcgatgt taacgaccgc gggctttacc gtcttggcga tctcgacgaa actcttggcg
      781 gtgccccccg tcaccggttg ctgcggcgcg gtcgcaacgg gatagactac gccgttagcc
      841 ggcgaatcgg ggcccgcgct gcccgaaggc atccagccca gatccgaagc cacgacgacg
      901 ccgatgactg caccgaacgc caataacaga tacggaacca atcctgaacc acccaatttc
      961 atcgctgttc cttattgtat ttcgccggca tagatgccca tgatcgtctg caaaaacttc
     1021 accgcctccg ccttcggacg ttggaaggag tttcgcccga tgatgctgcc aaatcctccg
     1081 ccgtcccgga tggcgcgggc ttcctcaaac acattcttat cctcgctttt ggcaccgccc
     1141 gagaaaatga cgatgcggcg cccgtcgaat gcgctttgca ccacatgttt gacgcgctcg
     1201 ctcagggtct tgatggaaat ctgtgccgac tcataaactt tcttagccgc cgcttgctca
     1261 aggtgggcag aggggagttt gaccttgatg acatgcgccc ctagttgcgc ggcaatttgc
     1321 gccgcatagg ccaccacatc catggcggtt tccccttcct tgctgatcgc cgacccacgg
     1381 ggataggacc acacgacgac ggcgagaccg ctgtcttttg cttcttcggc gatcgcgcgt
     1441 aattgctcat acatcagatt gcagtgggcc gatcccgggt aaatggtgta tccaacggcg
     1501 gcacaaccca aacgcaacgc gtccttcacg ctcccggtca cggacggcag tggatccttg
     1561 tcgtcatgca gcacgtcatg attgttcaac ttcaagataa gagggatttg cccggcaaac
     1621 tggctggcgc cagcttcgag gaaacccaga ggagccgcgt aagcattgca tccggcgtcg
     1681 atggccagtt gaaaatggta caaggggtcg taccccggcg ggttcggagc gaagctgcgg
     1741 gcgggcccat gttcaaagcc ttgatccact ggcaggatga cgagtttccc ggtccccgct
     1801 aatttcccgc tccggagcaa ccgggcgatg gtggttttgg ttccgggatt gtcgctggtg
     1861 tagtaactca ggatttcttg tacatgatcg gccatgccat tcccccgctg gtttcaacac
     1921 aaactggtta tgaaagtact gctgtcccct gcatgtactg ttcggcgtcc cgcacatcgt
     1981 cggtgctgcc gatgatcaga ggcactcgtt gatgcaactt cttcggctcg atgtcgagaa
     2041 tgcgcacggt accggtgctg gccctcccgc cggcctgttc gacgaccatt gccatcgggt
     2101 tggcctcata aagcagccgt agctttccct ccggcttgtt cacctcaccc ggatacaagt
     2161 agatgccgcc acccaagagc aaccggtgta catccgcgac aagacagcct gaataccggc
     2221 tgctatatgg ccggccgaca gccttgtccg acttcttgag cgagtcaatg taccgcttcg
     2281 tcccatcggg ccacttttga tagttaccct cattgacggc atacactttc cctcgcgagg
     2341 gtatcttgat gcctggatga gacagctgat actcgccgac gttgggatcc agtgtgaacc
     2401 cgtagacgcc cttgcctgtc gtgaacacga gcatggtgct cggtccgaac aagaaatagc
     2461 cagctgccag ctggtcggtt cccttctgca gcaagtcact ctcaccgggc aattgatcgg
     2521 ccttcttcgg cgccacgatg gaaaagatcg atcctagcgg catattgacg tccgtgttcg
     2581 acgagccatc caaggggtcg aaaagcacca tgtagggggc gggcctcccc ggagaggcga
     2641 cgaagaccgg cttttccatt tcttctgaag ccagtgcgcc ggccacacca tcacgctgga
     2701 gcacttcaac aaaagtctca ttggcaatct gatccaattt cttgaccgat tccccctgga
     2761 cattcgtttc tcccgtcatc cccaacatgt tgaggaggcc ggcaatccga aggtctcgag
     2821 ccagtgtctt tccgaccagg ccgatggctg aaagaaggtt ggtcagggat ccagcccctc
     2881 cgagtgaggc cggtggatta accatgagaa attgttcgac cgtgagtggc tggtgcgcca
     2941 tgagggagta ggtccgtatg aagtcgaaga gacccttctt cggaacaccc gaagcgtggg
     3001 cccccggtat gacaatgaat gtgagcgatt atagcccgtc tggggagcac gggcaacgaa
     3061 tctccgccct cttctatgga gaagactttg aggagaagcc atcgaccaaa tcggcgacga
     3121 tggagccaat aatgacctta gccttcatcc ggacagggat gcgccgctcg tcagtagtga
     3181 accagacgcg aatatccccc ttattgatga ataggccttt aaacggcatg atcacaacga
     3241 cttgcgcagt ctccgccttt ccccagaccc cctccactgt ctcgatgttc tcgacacgca
     3301 cctgcacctg ccggtttctt ttgtcgtgat agacattgag cgtcagagag gccccaggtt
     3361 tcatgggtag ttgattgcgt acgtaataca aacaggaaat gagatcttga gttccggcgg
     3421 ggatcgggag agtttcggta gtcccatgcc ggacggcggt cacggtcccc tcccgatggt
     3481 tgaacgtgta ttcgatatcc tccttattct tgccttcccg gcgtctgaag accatgtgct
     3541 gtggtagctg gacgctcagg tcgaactcgg attccacgcg gttgtcgaca ggaaaaacct
     3601 tactgatggc cggccgtgat tgagcggtcg tgaccagtct ggcgcgcgac cccgctccat
     3661 ccggctggac ttccatgacg gcggtccctg caaggacgtt caaccaggat atgtcgtaag
     3721 tgagccgctc accattctga accgcttgcg tgggtgcagt ctccacacct gtcgctgtcg
     3781 ccatctggtc gacgagcaac aacaggcacg tcagcgtaca ccgagcgaga atggctggca
     3841 tgttgacggt ctagagagag acagggaatg gaactaggag agcatggagc gcttcacttc
     3901 ggccaattcg ccctcaacga gggcccggat cgcggacaag cggtcggctg ttgtggcttc
     3961 gaatcggagg accaatgccg gctgcgtatt cgacgcccgg atcaaccccc acccgtcctc
     4021 aaagatggcc cgcactccgt caatcgtgac cacgtcacga acgacgtcgt tgtttgggcc
     4081 gatgggccga ctggacttcg catagccggc gagccggtcc tgaacctttt tgaccagctc
     4141 gaatttgatc gaatccggag tatccactcg aatttccggc gtgacggatg tggcgggcag
     4201 gtctgcgacc aatgtggaaa gcggtttgcc ggtttttgcc aatatctcaa cgaggcggca
     4261 ggaggcataa atcgcatcat catagccaaa gtatcgatca gcaaaaaaca tatgcccaga
     4321 catttctccc gccagcaccg ccttctcgct cttcattttc gctttgatga gggaatgacc
     4381 ggtcttccac atgatcgcgc ggccgccttg cttcgcgatg tcatcataga gacattgcga
     4441 cgccttgacc tcggaaataa tcgtgctgcc cggaatcgtc cgtaagatat cccgggcata
     4501 gaccaccata agacgatcgc cccacaagac gcggccttgc tcgtcaatgg cgccgatccg
     4561 atcggcgtct ccgtcgtagc cgatcccgac atccgccttg tgctgtttga ccgctttgac
     4621 caagtcgtcg agattttcca gcaccgtcgg atcaggatga tggttcggaa accggccgtc
     4681 cagatcacag tagagaccgg tcacgcggca gcccaaaatt tcgagcgctt ccttggcgac
     4741 caatgccgcc gcaccgttcc cacaatcgat gaccacatgc aaccgcccgg cattgacgcc
     4801 cttgaagctg gttcggaggt agtccagata gtcaggtatg atcgtatgct cgctgagccg
     4861 cccttgaccg gacgtgaaac ttcccgtttc caggatccgt cgaagctcca cgatggtctc
     4921 gccatggatg gcttccttgc ccacacagac tttaaatccg ttgtattcgg cggcattgtg
     4981 gctgcccgtg atcatgatgc cgccttggac cggaagctgg aacagcgaga aatagagcag
     5041 tggagacgga caaatgccga tgtcgatgac gttcacgccg ccggctagca agccgctgac
     5101 cagggatttg aacaaggcgg gagagctcag ccggccatcg cggccgacgg agatggtctg
     5161 aacaccacgt ttcttgacat acgtgcagta ggcgcgaccg acctgttcgg caatcgcctc
     5221 tgtcagttcc tcccccacaa tgccccgaag atcgtattct cgaaacagtc ccatcaatgc
     5281 tccctggcta tctggccgtc gttcgcccat aatcatcttt gaaacgcacg atgtcatctt
     5341 ccccaaggta aggaccgttc tgaacttcaa tgatgttcag cgtgtcctgt ccgaagtttt
     5401 caaggcggtg tttcgtctct acgggaatcg ccgtgctttg ccccacgtgc agttcgaaca
     5461 cctca
//
LOCUS       DASTQY010000279        28753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1028381, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000279 DASTQY010000000
VERSION     DASTQY010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 28753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28753
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028381"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFR82_09165"
     CDS             <1..308
                     /locus_tag="VFR82_09165"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEU4501807.1"
                     /translation="GPSAGAAMAVGFIAVFKGDRIQRGTALTGTLEPGGHIGQVGGIP
                     DKIRAAKREGYHTVLVPRGQLHTPEWNLIELGFQLNISVKEVDTVDEAYQLMTGNRI"
     gene            complement(346..816)
                     /locus_tag="VFR82_09170"
     CDS             complement(346..816)
                     /locus_tag="VFR82_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501808.1"
                     /translation="MSHFLFIAAALRERTSEESAELQLSHGLWGLCTALIRSNLQTYL
                     TPESCGLVYVLKAGLCAEFTIVPPPLDFAKLDEFLKDDLRTEARFGFVRVQGIKRWTW
                     SPQASQALLQHVLQIPDQAELARRLALGMHKLTAEEFKAIVEGVRIGTGSSYKM"
     gene            complement(916..1344)
                     /locus_tag="VFR82_09175"
     CDS             complement(916..1344)
                     /locus_tag="VFR82_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501809.1"
                     /translation="MAKMKKRPAKKQTAKTKTKKAAKKTARKPAKKQSAAKPKATPAK
                     KAGGAPVPSKPPVVKKVGPDEEIRKTTAKGAPAMTPKEPDLDEEEMGEEEIDLSGESE
                     SESEIDEEIEEDLSLDDDEEDVDYIEKSDDLIDDSDDYRH"
     gene            complement(1510..2634)
                     /locus_tag="VFR82_09180"
     CDS             complement(1510..2634)
                     /locus_tag="VFR82_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501810.1"
                     /translation="MFFTPDNHSTTQVKQGSSRQQGDILALPGLTQRLDCTILPPHSG
                     GGQMCRSIAIVLFLVVLSAVSGQSVGALTLTSPSQGATLQAGRPVTVEVFVGKEFNLR
                     TIHYYWYRPDEEPLASHQAHPAPFKPMTGGSPFAGTVLVPSDALGTMRLLAVGEVVRG
                     RLDSHEEFDEVVVAVETTAELQTIEFAVEKPWKMQQAGRRIVVPVIGQFSDGVWRPLS
                     GSSTGSRFHSSDERIVSVDATGVLQVIGGGKAIVTVENSGKAGAVEVIVQGDEEPNRP
                     PIAEVAKELHARSGELVLLDGLRSRDPDGDPLHFEWKQILGHRVILSNVNEAKATFIA
                     PKVSERKQYQFSLVVTDMAGPDVMKGADSVPAVVTVWITP"
     gene            2633..3019
                     /locus_tag="VFR82_09185"
     CDS             2633..3019
                     /locus_tag="VFR82_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501811.1"
                     /translation="MTIAFFRRTLVAMVAMLSLTAWPTTATPPYTAIIIDSGSPYFVP
                     KSATVSTGAPIRWENPTPTEHTVTHVGCLEDGAGCAFDSGLVLPNDSYTHPGLAPGKY
                     QYICRIHPIMHGTIIVTDSTPGPSQL"
     gene            3095..4585
                     /locus_tag="VFR82_09190"
     CDS             3095..4585
                     /locus_tag="VFR82_09190"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4501812.1"
                     /translation="MKPAVAVHEPRHLSGETLNLLAWHIPDLIAYQHHADEWWFAPLD
                     DNLPIVRLNRTGLEMLKAMNGHTTVRTLIDRYGSRICGPDGQPGQWHLERWALPNFSL
                     CYYGTEPPGGHRHKAKWDVLLQQIREGWSGQEGFEGEEHLQDFHVHDLKDSAGDDGHF
                     DLIETTVSHLFREPNEALNGLTYGRLVMQKLRRLGWFNPKPQVIVEVGGGLGYVAREL
                     GKELLPFERQSIKYCSVDITAPFLQLQLSRARAGGWQCAGTQANGEWLPFKDNSVDLV
                     IDNENMADMTPVKLTKQELLTGTGATAPHQEALDWIRRVRLPIGKDLPDDVIFNLGPF
                     RFVGELWRVLKPGGRAFLTEFGIEDGWPAPVKLPGHTEYEVQYSHLRQAVRWLGFQEQ
                     YVPLPQFLAMKPDTKVLCTGAAYTIQRFCQAMNKPFVVRAYTEQELKHALGDLLPKFQ
                     GTHYHDVADPAWFGLIDFKVLLLEKPGGAPKPTFTETKGYRWYSQR"
     gene            complement(4785..5726)
                     /locus_tag="VFR82_09195"
     CDS             complement(4785..5726)
                     /locus_tag="VFR82_09195"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEU4501813.1"
                     /translation="MTSKRLAGIDIGTLTCRLLIADLPANNHLNELCSERRILRLGEG
                     VDQSKRLKREAVDRVIQCLKDWRATIERYSVDAQVAVATSAVRDAANKQEFLNRVISE
                     AGLEVEILSGDEEARRTMLGIRSGLPEDIKDVLALDIGGGSTEFILARQGQPPVVDSI
                     DIGVVRLSERLLHHDPPTRHEIEQARAWIRRETTVVLAAMPRGTGLTFVGTAGTVTSL
                     AAMALQLPAYDAARIHNYRLGLNTITDLEQHLLSRSKAARIGMPGLEKNREDVIAAGA
                     IILRTVMDILGERECLVSDRGLREGVLIELATRHSRD"
     gene            complement(5723..6046)
                     /locus_tag="VFR82_09200"
     CDS             complement(5723..6046)
                     /locus_tag="VFR82_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501814.1"
                     /translation="MGLLQRLKHDLKTGLTTLRLGTAQAANRALEESELLRLRLEVRR
                     IDQQLKELYRDVGERAVSLREAGEPVERVLYDTEIARLVKDIHQLKATSGKLEAEMQE
                     IRDAE"
     gene            complement(6198..8735)
                     /gene="recG"
                     /locus_tag="VFR82_09205"
     CDS             complement(6198..8735)
                     /gene="recG"
                     /locus_tag="VFR82_09205"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU4501815.1"
                     /translation="MVFHRSAEAMAQFQQWLDRLARPIEFASRDAFARLPTVKNLSTF
                     VSSQILHALSHQVYPKSIQSALLKLRALFEEDHQRFTLEEQQRRLREAAAILRALREA
                     AGDQSRTWEELEPLTVPQPPPSKATRDIWAVPIRFAKGVGPQRGATLQRLGIETVEDA
                     LWTVPWRYEDRSVMTPIGQLVPGMVAAVFGTVVKNSAKRTRNRRFTVLEIGLEDQTGR
                     LQAVFFNQPYLEQTFAIGKSIMMSGRVIAGRQGWMVPRLDVSQYEVVGEGTESTLHVG
                     RIVPIYHETKGWTSRQMRVLMRGLVDEHAADLKDILPVPVRARQRLMPVQEAVAQVHF
                     PKAGADLAALDQGISPVHRRLAFEELFLLQVALAARQRSVQVEKKGLHFNPRSPLLDK
                     LGRVLPFKLTTAQDQVIREIFRDMIAPRPMNRLVQGDVGSGKTVVALHAMVLACGSGY
                     QTALMAPTEILAEQHYRNLSPLLRTLGVNTALLRGSDKAATKKNQIAGLASGHIQVAI
                     GTHALIQKGVAFKSLGLAVVDEQHKFGVLQRKTLIEKGYGPDVVVLTATPIPRTLAMT
                     VYGDLDVSIIHTLPPGRKPVRTFLFGEAQRRRAYQILRDELQNKRQAYVVYPLVEESE
                     KTDLQAAIQGAEQLQEGELKDYRVGVLHGRMKQADKESVMADFTRGKIQVLVATTVIE
                     VGVDVPNATVMMIEHAERFGLAQLHQLRGRVGRAGYQSYCLLMAATMGRNKTLPGKRS
                     TGNQESASLVKERLEALVRSNDGFVIAEEDLRIRGPGEFFGFRQWGMPEFRVANIVRD
                     GDLLQQARLEAFSLLKQDPQLGASAHQGIKEAMLRKWEKKLELGSVS"
     gene            9153..10589
                     /locus_tag="VFR82_09210"
     CDS             9153..10589
                     /locus_tag="VFR82_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501816.1"
                     /translation="MYRRNISYVLYPIAAVLGLVIVYQSIVKPTYISPLAAPHRDQGP
                     PETEKSSPVPLVEPLIEEPHVPAPKQLRVLEAPGVPEPKHRGSLETILEEIEKGHLKE
                     AEAALIHLPTTLAHDSRIYAAVLWNNLGIEQEKLEGTKGSLKTFKKAASLDPKNSTIQ
                     LNLAHAYWEQRDPAMSQDFLERLIALAPQEPFPHLALAELLHERGRLSEAARHLDQAA
                     ERLARDPSMQSYLQTVTSKMRRTDEMDSRLSSRDGAHFTVKYDGETDQATWTVVLDVL
                     EEAYREIGQKFGHFPAKTIVVVLHPRATFQGATGSPAWADGLFDPVLGRIHVPTQSAL
                     EDRAWLKRVLRHEFVHALLHDQQGLNHHAIPTWLNEGLAMQLSSDHWEDVDQLHQQQL
                     SIIPLTALEGSWGEFPSEAASVAYLEAESAAGYLINRYGMHQVQQLLARLKQKQSLPA
                     AIHAQLSVSYEQFQSRWMAHLHEGRPRG"
     gene            complement(10477..11394)
                     /locus_tag="VFR82_09215"
     CDS             complement(10477..11394)
                     /locus_tag="VFR82_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501817.1"
                     /translation="MRSTSRLSIRACLQHLFSLAIFLTFFEVGPVPATAGTTENPSMA
                     AGHARTVVAAGFGFQDDETSSITVKTYDAQSGEVLTAETYELDIKDDGPPTSHPKTRI
                     FAGGVGLGAGSLSEFTLRVYDAANGHFLWEGRLNLTIGDHPEATTHQVMAHVQPRAAV
                     TRIASSTKVTGQPYFVLRARHPETGQLMWSDEFSTEASTVRIERIGRSVIGVTGAAPR
                     DIDFRIMMPDQAGRRLLWEDKVVPEEEIEQSAVPERSDETAGRTPQGGRSAIPSVVPR
                     ADAPSNGIETARRTPTVEHESPRASFVFA"
     gene            complement(11567..11971)
                     /locus_tag="VFR82_09220"
     CDS             complement(11567..11971)
                     /locus_tag="VFR82_09220"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEU4501818.1"
                     /translation="MTEPFSKQSGQSLLVYDGQCRMCVAAKNGLEQLREESQADEVRL
                     IPYQSEEAKQVLGAHYRPGRPPVAFLVHPDRTIATGLDAFLPLLPGLKGGHPLARFLS
                     FPLVKPFAYLAYRLVARYRYRLFGEVPLKRPD"
     gene            complement(12318..13385)
                     /locus_tag="VFR82_09225"
     CDS             complement(12318..13385)
                     /locus_tag="VFR82_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU4501819.1"
                     /translation="MRQPIKTFDEFRDALSAYRLPRVILAALELKVFTVIGNTTWLLP
                     DLAREMKVSERGLSILCRNLAMAGLLYKQGNRYRNSPLAATALNADDPNYRGSYLDLI
                     TNHWSDWLRLLESVKTGEPLDKDEPEEPDYRRRFTWAMHYRTLETAPMIAAQLPLKGV
                     NTLLDLGGGPGTYAMAFLAKHPRLRATVCDRPAALEVAKEIAATHKAGARLSYMPLDL
                     LTDDIPGPFDVIWYSNVLHIYSPEQNQDVFRRAKAALADGGRLVIQDAFLHDREGLFP
                     EEATLFAVSMLLFTEGGNTYTVGETSAWLKAAGFDSVRPVKMKKGKEDWEGGILEAAV
                     LRGRPKRHGHRTRSATNSRSR"
     gene            complement(13382..13819)
                     /locus_tag="VFR82_09230"
     CDS             complement(13382..13819)
                     /locus_tag="VFR82_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501820.1"
                     /translation="MNDMKSPRWTEARSTLSKAFPQLYELEPDGPLALDLGQDGWLLE
                     ITPEGKLLCQYGVAMDDVMSLMSEGTPEDLGTDEVAKQAKYFIQPAVSKYRPLLLQSG
                     FSEQTEMNEEYVAVTFERQVDLENLSKVQDLMRWCCRQIGGAA"
     gene            complement(13816..14643)
                     /locus_tag="VFR82_09235"
     CDS             complement(13816..14643)
                     /locus_tag="VFR82_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4501821.1"
                     /translation="MILKNGLSEELNRQGNEHFGRGHYTEAYTCYAKALECDRLTGDQ
                     RALAATLGNLGNICAVSGRREAAQNYYQEVLELQKIFGDEKGIGTTLANLGNLRADAG
                     EWDRARAYYLEALDLMTKTQDEASKAVLFSDLGLIAREKGEFNEAIAHYEKSLVLMRR
                     LGNDGGVADAWRMLARTFLLQQRFDDAIACCQTSQSIAERSQDELRTGGARYVLADCY
                     EAMGRFQEAAQLLERVVEMDRKYHLPKLGENTKRLEGLRQRLSAEKPTTAARKESHG"
     gene            complement(14717..15310)
                     /locus_tag="VFR82_09240"
     CDS             complement(14717..15310)
                     /locus_tag="VFR82_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501822.1"
                     /translation="MKPICPGIWQWSWFSEEKQLNFNGLFLTVGEHRILVDPPPLTAE
                     ATTVIRREGRLDYIIVTNRDHAREAQVCRGDFGCQLFVPDADAPQMDLKPTRTFKDGE
                     LLPGGIWVIHLLDQKSAGESALFIQQGKGVLIVGDALIGKPPGALTMLPGEKYHDVVK
                     AKEGLRRLLKYNFDAVLVGDGTSIITGAKAAVEKALA"
     gene            complement(15353..15592)
                     /locus_tag="VFR82_09245"
     CDS             complement(15353..15592)
                     /locus_tag="VFR82_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501823.1"
                     /translation="MDEIEEGKQKFLELVKGIDQQVQVVIPVTPSNSMFLISLTKGAN
                     RKFITVPEDDIIDLPHESSVLTKVTKTIKDAVTAL"
     gene            complement(15748..16284)
                     /locus_tag="VFR82_09250"
     CDS             complement(15748..16284)
                     /locus_tag="VFR82_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU4501824.1"
                     /translation="MKRRWVILSVLLCPVLTAHLLLAAGFFKVGERAPSFALNAITGE
                     PVSLEANKGKVIVLGLFHICDPCMMQGTNLQKVYEATQGKNVAVIGVNSSGNSKKDVG
                     EFLSAFPVKVTYPYLLDPGKVTDKMYGGGKFIPNVYVIDQQGVIRWQRVGNMELAGSD
                     VIIAEVEKLLAADGGNKM"
     gene            complement(16591..17649)
                     /locus_tag="VFR82_09255"
     CDS             complement(16591..17649)
                     /locus_tag="VFR82_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501825.1"
                     /translation="MSQLVMGLRAESTTTVDSREYRFSFHGITFRFCSSSASLSSSLA
                     AWIRTVDCGDVKGDVSLTAHFEQVSSREAVPVSLSAGARRVFSGTMPALGNSLRALWQ
                     CDVYRDGERLVVDVRDYGVLLIDRNRSAASGHFYCPETMGPDRLETFFHYTMIELLKS
                     KNTFPLPAAALEFRGTGVLLHGPNGCGKTTAMLSLLRSGYRYLGDDISLLQDHGPMQL
                     LLGASVNIDVTDHTIEMFPELRTAGLGLLRQGAYRKSFHTEDVYPGSRGRSCEPAMIL
                     YLQVTNMSHSCLEPLAKSLALEALVRREAYRHDADTAAQEFQVLSKLVQRASCYRLHF
                     GRDVVDLPRLITPLLERH"
     gene            complement(17671..18057)
                     /locus_tag="VFR82_09260"
     CDS             complement(17671..18057)
                     /locus_tag="VFR82_09260"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEU4501826.1"
                     /translation="MSTPPLLPFMEGEGPQNWIPVLSSDGDHKPDWSRILHLAPTKNP
                     NVQGVVLEGETVLLDLVTGQSFRLNAVGTALWEHCTGTATLEQIHHVVSARLESHVGD
                     LREQMIACVVQWSHDGLLHRSDGPGR"
     gene            complement(18222..19652)
                     /gene="purF"
                     /locus_tag="VFR82_09265"
     CDS             complement(18222..19652)
                     /gene="purF"
                     /locus_tag="VFR82_09265"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU4501827.1"
                     /translation="MRKELPIVGSDKFHDECAVFGIYGHKEAANLTYLGLYALQHRGQ
                     EASGIVSADGDHFHVHKGMGLVADIYNTQTLKNLTGSMAIGHNRYSTAGGQDLKNVQP
                     LTVNFAFGNLALAHNGNLINAQVLRNELEAYGAIFQSTSDSEVIIHLIAHSRADTLLA
                     RVIDALSQVRGAFSVVLLTDEGIIAARDPHGFRPLSLGRLRDTYLVASETCAFDLLDA
                     EIVREIEPGELVVLNENGVKSYKPFSAVQPAMCIFEYVYFARPDSKIFGANAVYATRK
                     ALGRQLAQESWVPADLVIPVPDSGVPAALGYSEGAGIPFETGLIRNHYVGRTFIEPEQ
                     SIRHFGVKVKLNAVPEVLHGKRVVVVDDSLVRGTTSRKIVKMIRQAGAKEVHMRISSP
                     PIISPCFYGIDTPTKKELIASSHSAEEIRKYITADSLAYLSLDGMLKSAPGAPDHYCN
                     ACFTEQYPISFTRAEEFQLGLFERSS"
     gene            complement(19661..21910)
                     /gene="purL"
                     /locus_tag="VFR82_09270"
     CDS             complement(19661..21910)
                     /gene="purL"
                     /locus_tag="VFR82_09270"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEU4501828.1"
                     /translation="MHTNTADIKDLLAQHNLSEDEFKKIVDILGREPNLTELGMFSVM
                     WSEHCSYKSSRLHLKKLPTTGPRVVQGPGENAGAVDIGDGLCVVFKMESHNHPSFIEP
                     YQGAATGVGGILRDVFTMGARPIALLDSLRFGSLDSAKNRHLMRGVVAGIAGYGNCMG
                     VPTVGGEVRFDDIYSLNPLVNAFCLGIAHKDKIFRGTAAGVGNPVIYFGSKTGRDGIH
                     GATMASDAFDDESDQKRPTVQVGDPFQEKLLLEACLELMANDLLVGIQDMGAAGLTSS
                     SCEMASRAGNGMELDLTHVPRREQGMTPYELMLSESQERMLMVAQAGKEEECIRICRK
                     WDLDVAVVGKVTSDGLLRVIDQGKVVAEIPAKSLADDGPRYERPYSPPAYQDMLTNLT
                     YDSMPDVKDANAALLTVLASPTIASKRWVYEQYDHMVRTNTLVRPGSDAAVVRIKGTN
                     KAVAMTVDCNGRYCLLHPYEGTRLAVAEAARNLVCSGAEPIGLTDCLNFGNPERPDIM
                     WQFVLAIEGLKDACEHFQIPIVSGNVSFYNETNGLSIYPTPMLGMVGLIDKAEQAMTQ
                     WFRHDEDVIILLGKTRDDLGGSEYLKVMQHREQGSPPFLNLETEKALQDFVLKVIREG
                     LVESAHDCSDGGLAVALAESCVSGPDVRRGAVVKLELGSLRRDALLFGESPSRVILSI
                     RPDTVDTLLSRAADAGIPAGRIGTVGGDRFVIDVPKGRLSEGCRIDLPIDQVRDRWAN
                     ALEEQLSHE"
     gene            complement(21985..22701)
                     /gene="purQ"
                     /locus_tag="VFR82_09275"
     CDS             complement(21985..22701)
                     /gene="purQ"
                     /locus_tag="VFR82_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEU4501829.1"
                     /translation="MNIGVIVFPGSNCDHDCEHVFKDVLDQKVTMVWHKETSLAGLDG
                     VILPGGFSYGDYLRTGAIARFSPVMPAIKDFAKSGGLVIGVCNGFQILLEAGLLPGAM
                     LRNRSLHFVCKDVHVRVENAATPFTGACKPGQVLKIPIAHAEGNYYTDPVTLAGLQAN
                     AQIVFRYCTPEGQVTPEANPNGSLDNIAGILSAEGNVLGMMPHPERSAEELLGNADGR
                     MILASMVESLKKRPLKMVGA"
     gene            complement(22698..22943)
                     /gene="purS"
                     /locus_tag="VFR82_09280"
     CDS             complement(22698..22943)
                     /gene="purS"
                     /locus_tag="VFR82_09280"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEU4501830.1"
                     /translation="MKAKIHVTLKQGILDPQGKAIEHALDSLGFKNAANVRVGKYMEL
                     DVSEADKAKAEAQVKSMCEKLLANTIIEEYRYELVST"
     gene            complement(22940..23158)
                     /locus_tag="VFR82_09285"
     CDS             complement(22940..23158)
                     /locus_tag="VFR82_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501831.1"
                     /translation="MLKAFLNYGLVAAVLVWAGVVAMMAYRLDDSPWRWAFAALAVGG
                     IATVAVIFKIRRYVDGLNKQSGEQETKS"
     gene            complement(23412..24116)
                     /gene="purC"
                     /locus_tag="VFR82_09290"
     CDS             complement(23412..24116)
                     /gene="purC"
                     /locus_tag="VFR82_09290"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU4501832.1"
                     /translation="MTAGTLIYEGKAKKIFTTGHPDEVLQYFKDDATAFNAQKRGTIA
                     EKGIINNKVSERLFRLLEQNGVATHFVERKSDREMLTKKVTIVPVEVVVRNVIAGSLA
                     KRLGLKEGEGIQPPIVEWYYKNDALGDPLIADEHVRLLKLATPEQMAEIKRLALKVNS
                     VLQPFFAERRMVLVDFKLEFGVHNGRLILADEISPDTCRFWDQATRESMDKDRFRKDL
                     GKIEEAYQEVLKRVCG"
     gene            complement(24132..24926)
                     /locus_tag="VFR82_09295"
     CDS             complement(24132..24926)
                     /locus_tag="VFR82_09295"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEU4501833.1"
                     /translation="MTAQRAGFVSLITMSASLWAVLCFAAADLEKLLTEPGVYGTFAA
                     YSFDEEWAKEDEATRIAHLTVFKGVVEQHREKIAIDLYLMEGLSDHADILFRTHATEL
                     RDTQKFLVDLQSSVFGKHLKAAGVMHGLTKKANYVPGFPDQLKTDLKVTSDPGPKPYV
                     VIIPIRKSADWWALDQDKRAAMMKEHTEASLPYHKTVKRKLYHSTGLDDVDFITYFET
                     SKLEDFHGLILSLGKVKEFQYTRRFGHPTWLGTAKGLDEIIEILAQ"
     gene            complement(24923..26248)
                     /gene="purB"
                     /locus_tag="VFR82_09300"
     CDS             complement(24923..26248)
                     /gene="purB"
                     /locus_tag="VFR82_09300"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU4501834.1"
                     /translation="MIDRYTRPQMKAIWDLKHKYEIWLEVELQACAAFEKAGQAPRGT
                     SAKIRKKAKINVSRIAEIEKVTKHDVIAFLESLADTVGPEHRFLHMGLTSSDIVDTSL
                     AIQMTEALDLILEGVEGLLAVLKRQAFRYKSQVMVGRSHGIHGEPISFGFKLAIWHEE
                     VGRHLARLRQVRREIAVGKLSGAMGTFAHQGPDIEAYVCAKLGLTVDPVSNQVVQRDR
                     HAAYATSLALLAATIEKIATEIRHLQRTEVLEAEEYFSAGQKGSSAMPHKRNPIVSEN
                     LCGLARLVRANSFAAMENVALWHERDISHSSVERVIMPDSTILVDYMLAKVTDLIKHL
                     VVYPANMKRNLELTGGLVYSQRLLLALIDKGAQRKESYEAVQRNAMASWNGSGNLQEL
                     VSKDPFIAQHLKKSEIAACFNPKYYLRHVDEIFKRVFRSVGHAPQKGRT"
     gene            complement(26264..26722)
                     /gene="nusB"
                     /locus_tag="VFR82_09305"
     CDS             complement(26264..26722)
                     /gene="nusB"
                     /locus_tag="VFR82_09305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU4501835.1"
                     /translation="MGSRHQSRERALQILFQYDIHGKPGVWLDEFWNQFQATEDVRSF
                     AEQLVSGVLGHKNDLDALIGKYAMNWKVSRMQIVDRNILRLGAYELLWLDEVPAKVTV
                     NEAIELAKDFGDEEAAKFVNGILDKVLTTEGKLALKRQKAVRQPQGGREE"
     gene            complement(26725..27270)
                     /gene="ribH"
                     /locus_tag="VFR82_09310"
     CDS             complement(26725..27270)
                     /gene="ribH"
                     /locus_tag="VFR82_09310"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU4501836.1"
                     /translation="MKLRKGSHNATGLKFGIVVAKFNKFVTSKLLSSCIDGLTKHGVV
                     PEDIEVVRVPGAFEIPLVARTLAGTKRFDAVICLGAVIRGDTPHFDYISAEASRGIGQ
                     AALDADVPIIFGVLTTDTVAQAIVRAEPTKFNRGGEAAKSAIEMATVMRMIRGTETTA
                     PPKETAGKNGAARRPARKHKT"
     gene            complement(27343..28551)
                     /locus_tag="VFR82_09315"
     CDS             complement(27343..28551)
                     /locus_tag="VFR82_09315"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU4501837.1"
                     /translation="MQTPFDDIEDAIRDIKKGRFVILVDDEDRENEGDLVIAAEKVTP
                     HAINFMARHARGLICLALTPERVEELQLPQQAVENTAAFGTAFTVSIDARKDITTGIS
                     AADRATTIHVAIDPKSKPSDLARPGHIFPLKAQRGGVLRRAGQTEGSVDLARLAGLLP
                     AGVICEIMNEDGTMARVPELTKFAKTHKLKMVTVKALIEYRMRRETFIKRMASARLPT
                     IFGEFDAVAFENEIDHITHIALVKGRVDNGAPTLVRVHSGCLTADALGSLRCDCRDQL
                     HAAMEVIQKEGRGVLLYLNQEGRGIGLLNKIRAYGLQDKGSDTVEANLKLGFKADLRD
                     YGVGAQILANLGLHQIKLITNNPRKIVGIEGYGLKVVERVPIEIQPHAGNVKYLKTKK
                     KKLGHILKKV"
BASE COUNT         6018 a         8687 c         7687 g         6361 t
ORIGIN      
        1 atggtccaag cgccggtgcg gccatggccg taggatttat tgcagtgttc aagggagatc
       61 gcatccagcg cggcacggcg ctaaccggaa ctctcgagcc aggtgggcac atcggccaag
      121 tcggcggcat tcccgacaag atccgtgcgg ccaagcggga ggggtaccat acggtgctcg
      181 tgccacgggg acaacttcac acgcccgaat ggaatcttat cgaactcggg tttcaactca
      241 acatatccgt caaggaagtg gacacggtcg atgaggcgta tcagctcatg acgggcaata
      301 gaatttagcg gaatggagaa tcgttgcgtg agtactccct gctccctaca tcttgtaact
      361 tgatcccgtg ccgattcgga ctccttccac gatcgccttg aattcttccg ccgtcagctt
      421 atgcatgccc aaggcgagtc gtcgagccaa ttcagcctga tctggaatct gaagcacatg
      481 ctgtagcaat gcctgactcg cctgcggaga ccaggtccac cgcttgatgc cctggactcg
      541 gacaaagccg aatcgcgcct cggtccgcag atcatctttg agaaattcat ccagtttggc
      601 aaagtccaac ggcggaggca cgatcgtaaa ctccgcacac aagccggcct tcagcacata
      661 caccaaacca catgattcag gcgtgagata cgtttgcaga ttgcttcgaa tcagcgcggt
      721 acacagcccc cataggccat gagaaagctg cagctccgca ctctcctccg acgtccgttc
      781 gcgcagcgcg gccgcgatga acagaaaatg gctcacgaga tgaccctttc catatgagag
      841 tccgcgagcg cggcgaccgc agcgatgggg tgaaggggaa gctgtcaggc cggatgtgag
      901 gtgaggctac atgactcagt gacggtagtc gtctgaatcg tcaatcaggt cgtcggattt
      961 ttcgatataa tcgacgtctt cttcatcgtc gtcgagcgag agatcctctt caatctcttc
     1021 gtcgatttca ctttcactct cactctcgcc gctgagatcg atctcttcct cacccatctc
     1081 ctcttcatcc agatccggct cttttggcgt catcgccgga gcgcccttcg ccgtcgtctt
     1141 cctgatttcc tcgtcaggac caaccttttt tacgaccggg ggcttggatg gaacgggcgc
     1201 tccaccggcc ttctttgcgg gagtcgcctt cggcttggca gcggactgct tcttcgccgg
     1261 ctttcttgct gtcttcttcg cggccttttt ggtcttcgtc ttggcggttt gtttcttcgc
     1321 cggacgcttt ttcatcttcg ccatcgatct ctctcctctc tcatccactg acacttgatg
     1381 taatgcggcc tccatctagc atgaacgtac gtagtcttgc aaccaggggt ctgccgccgc
     1441 gtcgttcggc gtgagagcag ctctggtcgg ccttgggagg gcgctcgagt ccttctctct
     1501 gtacggctct cacggtgtga tccacacggt cacgaccgcc ggcacactgt ccgctccctt
     1561 catgacgtcc ggaccggcca tatcggtcac gacgagagaa aattgatact gtttccgttc
     1621 cgagaccttc ggcgcgataa atgtcgcctt ggcttcgttc acattgctca aaatcacacg
     1681 atggcccagg atctgtttcc attcgaaatg caaaggatcg ccgtccggat cccggcttcg
     1741 aagaccgtcg agcaggacca gctcgccgga tctggcgtgc agctccttag ccacttcagc
     1801 gataggcggg cgattgggtt cttcatcgcc ttgaacgatc acctcgaccg cgccggcctt
     1861 tccactattt tccacagtga cgatcgcctt gccgccgcca ataacctgca agactcccgt
     1921 ggcatcaacg gacacgattc gttcatccga agaatgaaac cggctgcctg tgctcgatcc
     1981 ggagagcgga cgccacactc cgtcgctgaa ctgcccaatg accggaacga cgatccgcct
     2041 gccggcttgt tgcattttcc aaggtttctc gactgcaaat tcgatcgttt gcagttcagc
     2101 agtcgtctcc accgcgacga ccacttcgtc gaactcttcg tgactgtcta accggcctct
     2161 gacgacctct cccaccgcca gtagccgcat cgtgcccagc gcatcggagg gcacaaggac
     2221 cgttcctgca aaaggagagc cacctgtcat cggcttaaac ggagcaggat gagcttgatg
     2281 ggacgccaga ggttcttcat ccgggcggta ccaataatag tggatcgttc gcagattgaa
     2341 ctccttcccc acaaacacct caacagtgac aggtcggcct gcctgcaatg tcgcgccctg
     2401 ggaaggcgat gtgagggtga gcgccccaac tgattgtccg cttaccgcgc tcaagaccac
     2461 aagaaacaag acgatcgcga tcgatcggca catctggcca cctccggaat gaggcggaag
     2521 aatcgtacaa tccagccgct gcgtcaagcc tggcaacgca agaatatcgc cctgttgacg
     2581 actacttccc tgcttgacct gcgtggtaga atgattatca ggagtaaaga acatgacaat
     2641 cgccttcttc agacgcaccc tcgttgcaat ggtggccatg ctgtccctga ctgcctggcc
     2701 cactaccgcc acgcctccct acacggccat catcatcgat agcggttcac catattttgt
     2761 tcccaaatca gccacagtct cgaccggtgc gcccattcga tgggagaatc ccacgccgac
     2821 cgagcatacc gtcacgcacg tcggctgttt agaggacgga gcgggttgtg cctttgattc
     2881 aggtcttgtt ctcccgaacg attcctatac gcatccgggc ctcgcccccg ggaagtatca
     2941 atacatctgc cgcatccatc caatcatgca cggaaccatc atcgtgacgg actccactcc
     3001 agggccttct caactataat cccgccacgg cgcctgggtt cttgcacgaa aacgatccgc
     3061 tcgtgtacgc ttcccgcgcg tatcgaggac caccatgaag ccagctgtcg ctgtccacga
     3121 acctcgccat ctaagtggag aaaccctcaa cctgctggcc tggcacattc ccgatttgat
     3181 tgcctatcaa catcatgccg atgaatggtg gtttgcccct ctcgacgaca atcttcccat
     3241 tgtgcggttg aaccgcactg ggctcgagat gctcaaggcc atgaacggcc acacgaccgt
     3301 ccgcaccctg atcgaccggt acggctcaag aatctgcggg ccggacggcc aaccaggaca
     3361 gtggcatctc gaacggtggg ccttacctaa cttttcgttg tgctattacg ggacagagcc
     3421 tcccggcggg caccggcata aggccaaatg ggatgtcctc ctgcagcaga tacgcgaagg
     3481 gtggtccgga caagaggggt tcgagggtga agaacatttg caagatttcc atgtgcacga
     3541 cctgaaagac agcgctgggg atgacgggca tttcgacctg attgaaacga ccgtgtccca
     3601 cctgtttcgc gaacccaacg aagccttgaa cggcctgacc tatggcaggc tggtgatgca
     3661 aaaactccgg cgcctcggtt ggtttaatcc gaaaccgcag gtcatcgtcg aagtgggcgg
     3721 cggcctcggt tatgtcgccc gtgaactcgg caaagaattg ttgccgtttg aaaggcagag
     3781 tatcaagtac tgttcggttg atattacggc accattttta cagctccaac tctcccgcgc
     3841 gagagcgggt ggctggcagt gcgccggcac ccaggcgaac ggagaatggc tgcctttcaa
     3901 ggataattcc gtcgatctgg tcatcgacaa cgaaaacatg gccgacatga cgcctgtcaa
     3961 actgacgaag caagaattac tgacaggcac cggcgctacc gcgccacatc aggaggcgct
     4021 ggattggatc aggcgcgttc gtcttcccat cggcaaggac ctgccggacg acgtcatctt
     4081 caacctgggt ccttttcgat tcgtcggtga gctgtggcgc gtgctgaaac cgggcggacg
     4141 agctttcctg accgagtttg gcatcgaaga tggctggccg gcgccggtca aactccccgg
     4201 acataccgaa tatgaagtcc aatacagcca tctgcggcag gctgtccgat ggctcgggtt
     4261 tcaagaacag tatgtaccgc tcccacaatt cttggctatg aaaccggaca cgaaagtctt
     4321 gtgcaccggg gccgcgtata cgatccaacg gttttgccag gcgatgaaca agcctttcgt
     4381 cgtgcgcgcc tatacggaac aggaactgaa gcatgcgttg ggcgatctgc ttcctaagtt
     4441 tcaaggcaca cattaccatg acgtggcaga cccggcctgg ttcggcctca tcgatttcaa
     4501 agtgctgtta ttggagaaac caggcggggc gccaaaacca acctttaccg aaaccaaagg
     4561 ttatcgctgg tacagtcagc ggtgatgcgg accaactgcc cttcctgctg gggaatagcc
     4621 ccgtatctga agactgtacc tcctttagtc gcaccaagaa agggagggtc agtctggcca
     4681 gtgcttgtgg gaacgcggca cagcagacca atcgtatatc cagggaggcg ggtggtcgtt
     4741 catgcgcgta cgttcgaact catccgcggt ccattgaagc acgtttaatc tctcgaatgt
     4801 cgcgtagcca gttcgatgag taccccttca cgtaatccac gatcactcac gagacattcg
     4861 cgttcgccca ggatgtccat caccgtccgg aggatgatgg ctccggccgc aatcacatct
     4921 tcgcggtttt tttccaaccc cggcatgccg attcgggctg ctttgcttcg gctcagcagg
     4981 tgctgctcga ggtcggtgat tgtattcaac cccagccgat aattatgaat ccttgctgca
     5041 tcataggcag gaagctgcag cgccatggcc gccaaggagg tgaccgtccc ggcggttcca
     5101 acaaacgtga ggccagtccc tcgcggcatt gcagccaaca ccacggttgt ttctcttcgt
     5161 atccaagcac gtgcctgctc tatctcgtgt ctagtcggcg gatcatgatg caagagacgt
     5221 tcggaaagcc ggacgacgcc gatatcgatc gagtccacga cgggcggctg cccctgccgt
     5281 gccaggatga attctgtact tccgccgccg atatccagcg cgaggacgtc tttgatgtct
     5341 tcgggcaggc ccgaacggat gccgagcatc gtccggcgcg cttcttcatc gccgctgaga
     5401 atttcgactt ccaagcctgc ttcggatatg actcgattca gaaactcctg cttgttagca
     5461 gcatctcgga cagcgctcgt cgccaccgca acttgtgcat cgacactgta gcgctcgata
     5521 gtggcccgcc agtctttcag acattgaatg acccgatcta cggcctcacg tttcaatcgt
     5581 ttcgattgat cgacgccttc tcccaatcgg agaattcggc gctcggagca cagttcattc
     5641 aggtggttat tggcaggcag gtcggcaatc aagagtcggc aggtcagcgt tccgatatcg
     5701 atgccggcga gacgcttcga ggtcattctg cgtcgcggat ttcctgcatc tcagcttcca
     5761 gtttgccgct cgttgctttc agctgatgga tgtctttgac gagtcgggca atctccgtgt
     5821 cataaaggac gcgttccacc ggttcacccg cctcacgaag actgaccgcg cgctcgccga
     5881 catcacgata caactccttc aactgctggt cgatccttct gacctcgagg cgcaggcgca
     5941 acagttcgct ctcttcaagc gcgcggttgg ccgcttgcgc ggttcccaac cgcagtgtgg
     6001 tcaacccggt tttcaagtca tgttttagtc gttgcagcaa ccccatcttc gcttcctctc
     6061 atacacaggg atcggttctc ttttcaggat gataatagga tgcccaagac gaagctcgga
     6121 acagaggaga caccgtctcc ccccttcaag gtctaccatc tagggagccg gcgaccatcc
     6181 accttgtctg cccatgctca actcaccgat cccaactcca actttttctc ccacttccgg
     6241 agcatcgcct ccttgatgcc ttgatgagcg gacgcaccga gctgtggatc ctgcttcaac
     6301 aaagagaagg cttccagcct cgcctgctgc aagaggtcgc catcccgtac gatgttggca
     6361 acgcgaaatt cgggcattcc ccattggcga aaaccaaaaa attcccccgg cccccggatc
     6421 cgtaaatcct cttctgcgat cacgaaccca tcgttcgaac gcaccaacgc ctcgagccgc
     6481 tccttgacca gtgacgccga ctcttgattc cccgtcgaac gcttaccggg caatgtcttg
     6541 ttgcgtccca tcgtagccgc catcagcagg caataggatt ggtaaccagc gcgtcctact
     6601 cgtcctcgca attgatgcag ctgcgcaagc ccaaatcgct cggcgtgctc gatcatcatg
     6661 acggttgcat tcggcacgtc aacaccgacc tcaatgacag tcgtcgccac gagtacttga
     6721 atcttaccgc gggtaaagtc ggccatgacc gattccttgt ccgcttgctt catccgccca
     6781 tgcaggacgc caactcgata atctttgagt tctccctcct gcaattgctc cgctccctgg
     6841 atggcggcct gcaaatcggt tttctccgac tcctcgacga gcggatacac cacgtacgct
     6901 tgccgtttat tctgcagctc gtcccggagg atctggtaag cgcggcgccg ttgcgcctcg
     6961 ccgaaaagaa acgtacgcac gggcttccgg ccgggaggca gcgtgtggat aatcgacacg
     7021 tcaaggtccc catagacggt cattgccagc gtgcgaggaa tgggggtggc ggtcaacaca
     7081 accacgtccg gcccgtagcc cttctcgatc agcgttttgc gttgcagcac gccgaactta
     7141 tgttgttcat ccacgaccgc caatcccaaa cttttgaacg cgacgccctt ctgaatcaag
     7201 gcgtgcgtgc cgatagcgac ttggatgtgc ccagatgcca ggcccgcgat ttggtttttt
     7261 ttcgtcgccg ctttgtcact gcctcgaagc agcgccgtgt tgacaccaag agtgcgcaaa
     7321 agagggctga gattccggta gtgctgttca gcgagaattt ctgtgggggc catcagtgcc
     7381 gtctgatacc ccgatccgca ggcgagaacc atcgcatgca gggccacaac tgttttccca
     7441 gaccctacgt cgccttgcac caagcgattc atcggacgcg gcgcaatcat gtcgcggaag
     7501 atttcccgga tgacttggtc ctgagcggtg gtcagcttaa agggaagaac acgtcccagc
     7561 ttgtccaaaa gtggagagcg aggattaaaa tgcagcccct tcttttctac ttggacggac
     7621 cgttgtctcg cagccaaagc cacctgcagc agaaacagtt cctcgaacgc cagtcggcga
     7681 tggaccggtg atattccctg gtcgagcgcg gccaggtcgg cgccggcctt tggaaaatgg
     7741 acctgggcta ccgcctcctg aacaggcatc aatcgttggc gggcgcggac aggcaccggc
     7801 aagatatctt tcaggtcggc cgcatgctcg tccaccaatc ctctcatgag cacgcgcatc
     7861 tgacgggacg tccacccttt ggtttcatgg taaatgggca caatgcgccc cacgtgcaac
     7921 gtcgactcgg tgccttctcc aacgacttcg tactgcgaaa cgtccaacct cggcaccatc
     7981 cagccctgcc ggccggcaat gacgcgcccg ctcatcatga tcgactttcc gatggcaaat
     8041 gtctgctcca ggtacggctg attgaaaaac accgcctgca ggcgcccagt ttgatcctcc
     8101 aagcctattt ccaagactgt aaagcgtctg ttccttgtcc gtttggctga attcttgacc
     8161 accgtgccga aaaccgctgc caccatgccc ggaaccaatt ggccgatggg cgtcatcacc
     8221 gaacgatctt catagcgcca cggcacagtc cagagcgcat cttcgacggt ctcgatgcct
     8281 aaccgttgca aggtcgcgcc gcgttgtggg ccgacgccct tcgcgaatcg aatcggcaca
     8341 gcccagatat ctcgtgtcgc tttggaaggt ggcggttgcg gcaccgtaag cggttccagc
     8401 tcctcccagg tgcggctctg atctcccgcc gcctcccgca acgccctcaa aatagcggca
     8461 gcctctcgta accggcgctg ctgctcctcg agagtgaagc gctggtgatc ctcctcgaac
     8521 agtgcgcgca gttttagcag cgcggattgt atagatttcg gatagacctg atgtgacagc
     8581 gcatggagaa tttgcgatga gacgaaggta ctgagattct tgacggtggg caggcgggca
     8641 aacgcgtcgc gactggcgaa ttcgattgga cgtgcgagac ggtccaacca ttgctgaaac
     8701 tgggccatgg cttcagccga ccggtgaaag accattgctg tgcgttgatg acgtgaattg
     8761 gagcagtatc gcaggggtag aacttgcttt aaggacagtc acaaggccca accgtctcac
     8821 gcgcccaacc ccgacccgcc aagatccgtg cctcccgcgc gaggcagcag catctgcgaa
     8881 tatggcgcca ttctccttca ggggacgagg cgagaacgag gctaggtgaa aagcgcctct
     8941 cgtgcctggg ttggtgggcg agaagaacgg ctgtttcact attctgctcg gctcggagga
     9001 gcgccacatt gggcgagatg gtatcacagc ccctcttccc cgctcaacga gagccgtgcg
     9061 tggctgcgat cccgaaccct gtagaattcc ttacttttct ttggcgtgcg ttcagcctgg
     9121 tgctagactg gcgacaaggt taaagacgcc ccatgtaccg ccggaatatt tcctacgtcc
     9181 tctatccgat tgcagccgta ctcggtttgg ttattgtcta tcagtcgatc gtcaagccga
     9241 cgtatatctc tccgctggcg gcgccgcacc gggatcaggg accgcctgag acggaaaaat
     9301 cgtctcccgt gccgttggtg gaaccgctga tcgaggagcc tcacgtccct gctccaaaac
     9361 aattgcgagt cctcgaggcg cccggcgtgc cggagcccaa acaccggggc tcgttggaaa
     9421 ccatcctgga ggaaatcgaa aaaggccacc tcaaagaagc tgaggccgca ttaatccacc
     9481 ttcccaccac gcttgcgcac gattcccgca tctacgcagc agtcctctgg aacaatctcg
     9541 gcatcgaaca agaaaaactc gagggcacta agggctcgct caagacattc aaaaaagccg
     9601 catccctgga tccgaagaat tccaccatcc agttaaacct ggctcacgcc tattgggaac
     9661 agcgcgaccc ggccatgagc caggatttct tggagcggct gatcgccctc gcgccgcagg
     9721 aaccttttcc gcacttggcc ttagcggagt tgcttcatga acggggccgt ctgagtgaag
     9781 cggcgcgaca cctcgaccag gctgctgaac ggctggcaag ggatccgtcc atgcaatcgt
     9841 atctccagac ggtgacatcg aagatgcggc gcaccgacga aatggatagc cggctgtcga
     9901 gccgcgacgg cgctcatttc acggtcaagt atgacggaga aacggatcaa gccacctgga
     9961 cggtggtcct ggatgtactg gaggaggcct accgtgagat cggtcaaaag tttggtcact
    10021 tccctgcaaa gaccattgtg gtggtcctgc atccgagagc gacctttcaa ggcgcgacgg
    10081 gaagtcccgc ctgggccgat ggactgtttg atccggtgct cgggcgcatt catgtaccga
    10141 cacaaagtgc gctggaggat cgcgcctggc tcaagcgggt gctccggcat gaatttgttc
    10201 atgcactact gcacgatcaa cagggactca accaccatgc cattccaacc tggctcaatg
    10261 aaggactggc catgcagctt tccagcgacc attgggagga cgtggaccag cttcatcagc
    10321 aacagctgtc gatcattccc ttaacggcgt tagaaggcag ttggggggaa tttccgagtg
    10381 aggcggcgtc ggtggcttat ctcgaggctg agtcggcagc aggctatttg atcaatcgct
    10441 atggcatgca ccaggtgcaa cagttgctgg ccaggctcaa gcaaaaacaa agcttgcccg
    10501 cggcgattca tgctcaactg tcggtgtcct acgagcagtt tcaatcccgt tggatggcgc
    10561 atctgcacga gggacgaccg aggggatagc agatcgacct ccctgaggcg tcctacctgc
    10621 cgtttcatcc gagcgttccg gcacggcgga ctgctcgatt tcctcttccg gaacgacctt
    10681 gtcttcccac aataatcggc ggccggcctg atcaggcatc atgatccgga aatcgatatc
    10741 tcgaggtgcg gcaccggtca caccgatgac gctccggccg atgcgctcga tccgaactgt
    10801 cgacgcctcg gtggaaaatt catcggacca catgagttgt cccgtttcgg gatgacgcgc
    10861 tcgcagcacg aagtacggct gtccggtcac ctttgtagag ctcgcgatcc tcgtcactgc
    10921 agctcgtggc tgaacgtgcg ccattacttg atgtgtggtt gcttctggat gatctccaat
    10981 ggtcagattc aggcggcctt cccacagaaa atgcccgttc gcggcatcat agacacgaag
    11041 agtgaactcg gaaagactcc ctgccccaag gccgaccccc ccggcaaaga tgcgcgtttt
    11101 agggtggctg gtgggtggtc cgtcatcttt gatgtccagt tcgtaggttt ctgctgtgag
    11161 gacttcgcca gactgtgcat cataagtctt gacggtgatt gatgaggtct cgtcgtcctg
    11221 aaagccaaat cccgccgcaa cgaccgtccg ggcatgcccc gctgccatgg acggattttc
    11281 cgtcgtccca gccgtagccg gaacaggtcc cacctcaaag aatgtcagga aaatcgcgag
    11341 agaaaacagg tgctgcaggc aagctcgaat ggagagacgg gaggtagacc gcatgcgtgt
    11401 tctcctgacg cgtggtctcg ttatctgggc cgactatgac aggatccctg gcaaggtgac
    11461 attcctctct gtggggagac aatcccccaa aagtgggtgt ccccaggatg gatgatggag
    11521 gacatggatc ggcggagctt cgagacgact gagtggaatt cgtcgtctaa tccggcctct
    11581 tcaaaggcac ttcgccgaag agacggtatc gatagcgtgc gacgagccgg taggcaaggt
    11641 aggcaaaagg cttcacaagt ggaaaggaaa ggaacctggc cagcggatgc ccccccttta
    11701 atccgggcag caaaggaaga aacgcatcga gtcctgtggc aatggttcga tcaggatgga
    11761 cgagaaacgc gacaggtggc cggcccggcc tgtagtgggc tcccaggact tgcttggctt
    11821 cttcgctctg atacgggatc agacgaactt cgtcggcttg gctctcctca cggagttgct
    11881 ccaggccgtt cttggccgcc acgcacatcc ggcattgacc gtcatagaca agcaggctct
    11941 ggcctgactg cttcgagaag ggttctgtca taggcccctc agcgaccccg ttgcccgttc
    12001 gatgcgcgac cggtatgatc cggtcatgcg ccttcgggtg atcctggcgg ccacgctcct
    12061 gtctgtcagc gttattggcg tatcaacaat agggttggct ttctcgcccg aactgccatc
    12121 aacggcttac ctactatggt ggctctttct cgtgttgatt ccgctggttc tcgccagctt
    12181 tgtgatgctg ggatggactt ggccctccat ggcttgcgtg gtctacggca ccattggatt
    12241 ggctatcgat ctgtccacgc tcgtgccgct cctcggaagg ctgcacgaat cgagtctcaa
    12301 ggtcgtcctc agtacactca gcgggagcgc gaatttgttg ctgatcgtgt tcggtggccg
    12361 tgccttttgg gccgccctcg aagaactgcg gcctccagaa tcccgccctc ccagtcctcc
    12421 ttccccttct tcatcttgac cggcctgacc gaatcgaatc ctgcggcctt cagccaggcg
    12481 ctcgtttcgc ccaccgtgta ggtattcccc ccctcggtga agagcagcat ggacacggca
    12541 aacagagtgg cttcttcggg aaacaatccc tcccggtcgt gcaggaaggc atcttgaatc
    12601 acgagacggc cgccatcggc cagggccgcc ttggcgcggc gaaacacatc ctgattctgc
    12661 tcgggcgaat aaatgtgcag gacattcgaa taccaaatca catcgaatgg acccggaata
    12721 tcgtctgtca acaaatccag cggcatgtac gacagccggg cgccggcctt gtgggtcgct
    12781 gcgatttcct ttgccacttc caacgctgca ggacggtcgc acacagtcgc acgaaggcgg
    12841 ggatgcttcg ccaaaaaggc catggcatag gttcctggcc ctcctcccaa atcgagtaac
    12901 gtgttgacac ctttcaaggg aagttgggcg gcaatcatag gtgccgtttc caacgtgcga
    12961 taatgcatgg cccatgtaaa ccgccggcgg taatcgggct cttccggttc atccttgtcc
    13021 agaggttcac cggtcttgac cgactcgagc agccgcaacc aatcagacca gtgattcgtg
    13081 atgaggtcga gataactgcc gcgataattg ggatcatcgg cattcagcgc ggttgccgcc
    13141 aaagggctat tgcgatacct attgccctgt ttgtacagca atcccgccat ggcgagattt
    13201 cggcagagga tggacagtcc ccgctcgctc actttcatct cgcgcgcgag atcggggagc
    13261 agccaggtgg tgttgccgat tacggtaaac accttcagtt caagagcagc caggatgaca
    13321 cgcggcaagc gatacgccga caaggcgtcc cgaaactcat cgaacgtttt gatcggttgt
    13381 ctcacgccgc tccgccgatc tgccggcagc accagcgcat gaggtcctga accttggaca
    13441 agttctcgag atcaacctgg cgctcgaagg tgaccgccac gtactcctca ttcatctcgg
    13501 tttgctcgga aaaaccggac tggagcagca agggacggta ttttgaaact gccggttgga
    13561 tgaagtactt cgcttgcttg gcaacttcgt ccgtgcccaa atcttccggc gtgccctccg
    13621 acatcaagct catcacatcg tccatcgcga cgccatactg acagagcagc ttgccttccg
    13681 gcgtgatctc gagcagccag ccgtcctggc cgagatcgag agcgaggggg ccgtctggtt
    13741 cgagctcgta gagctgtgga aaggccttcg acaacgtgct gcgtgcctcg gtccatcgag
    13801 gcgacttcat gtcattcatc cgtgagattc cttacgagcg gcagtcgtcg gtttttcggc
    13861 ggaaagccgt tggcggagac cctcgagccg tttcgtattc tctccgagtt taggcaaatg
    13921 atacttgcgg tccatctcca caacccgctc gagcaattgc gccgcttcct ggaatcggcc
    13981 catcgcctca tagcagtcgg caagaacata ccgcgcacca ccggtacgca gttcatcctg
    14041 cgagcgttcg gcaatagact ggctggtctg acagcaagcg atcgcgtcgt caaagcgttg
    14101 ttgcagcaaa aaggtccgcg ccaacatgcg ccaggcatcc gcgacgcccc catcgttgcc
    14161 cagtctccgc atcaagacca acgacttctc gtaatgggca atcgcctcgt taaactctcc
    14221 tttttccctc gcaatcaacc ctaaatccga aaacaggaca gctttcgagg cctcgtcctg
    14281 cgtcttggtc atgagatcga gtgcttccag gtagtacgct cgggcccggt cccactcccc
    14341 tgcatctgcg cgcagatttc ccaagttggc cagggttgtc ccgataccct tctcatcgcc
    14401 gaaaattttt tgtagctcga ggacttcctg gtaataattc tgtgccgcct cgcgacgacc
    14461 gctcacggca caaatattac ccaaattgcc cagcgttgcc gccagcgcac gttgatcacc
    14521 ggtgaggcgg tcgcattcaa gagccttggc gtagcacgta taggcctcgg tgtagtgacc
    14581 gcggccgaaa tgctcattgc cctggcggtt caattcttcg gacagaccat ttttcagtat
    14641 catgacttgc cggaggcggt ccctgcgaga ctttcagcca ttatcctttc ggcatccaga
    14701 ggttgcagaa tcaactctag gccagggctt tttcaacggc cgccttggcg cctgtgatga
    14761 tggacgtccc atcgcccacc aacacagcgt cgaaattata cttgaggaga cgtcggagcc
    14821 cttctttcgc tttgacgaca tcgtggtatt tttctccagg caacatggtg agcgctcccg
    14881 gaggcttgcc aatcaatgca tcaccgacga tgagcacccc cttgccctgc tgaatgaaca
    14941 gggccgattc gccggccgac ttttgatcca ataaatgaat gacccagatc ccgccgggga
    15001 gtagctcgcc gtctttgaat gttctagtcg gcttcagatc catctgggga gcatcagcat
    15061 ccggcacgaa caactggcac ccgaaatctc cccgacacac ttgagcctct cgtgcgtgat
    15121 cacgattcgt cacgataatg tagtcgagcc ggccctccct acggatcact gtcgtggcct
    15181 ccgccgtcaa aggcggcggg tcaaccagaa tcctgtgttc tccgacggtg agaaacaggc
    15241 cattgaaatt gagctgcttc tcttcggaaa accaggacca ttgccagatg ccaggacaaa
    15301 ttggtttcat gaaggatgtt cgccgatagg ccgaatcaag gacacaggcg ttttacaaag
    15361 ctgtcacggc gtctttgatc gtcttcgtaa cttttgtgag gacgctggat tcatgaggca
    15421 gatcgatgat gtcgtcctcc gggaccgtga tgaattttcg attggctccc ttggtcagag
    15481 aaatcaggaa catgctgttc gagggcgtga cagggatcac gacttgcacc tgctgatcta
    15541 tacccttcac gagttcaaga aacttctgtt ttccctcttc aatctcatcc attctttctc
    15601 ttccttttct ccgtcatcct cggccgccca tgcgcgatgg ctgagaactc tcaacctgcg
    15661 cgcctgaccg agcaccaacc ttaacaattc gatagtgttt ggtgtgccgc ggtcggcgag
    15721 cagagaacgt cccagccgtc acgctcctca catcttgtta ccgccatccg ccgccaacaa
    15781 cttctcgacc tcagcaataa tgacgtctga accggccagc tccatattgc cgacccgctg
    15841 ccagcggatg acgccctgct gatcgatgac atacacattg ggaatgaact tccccccgcc
    15901 gtacattttg tctgtgacct tgccggggtc cagaagatac ggatacgtca ccttcacagg
    15961 gaacgcggaa agaaactcgc cgacgtcttt tttggaattt ccggatgaat tcactccgat
    16021 gactgccaca ttcttaccct gcgtcgcctc gtacaccttt tggagattcg tgccctgcat
    16081 catgcaggga tcgcagatgt ggaagaggcc aaggacgatc acctttccct tgttcgcttc
    16141 caaggagacc ggctcgccgg taatggcatt cagggcaaac gaaggcgctc tctccccgac
    16201 cttgaaaaac ccggctgcca acagcagatg agcggtgagc acaggacata aaaggacact
    16261 taatatgacc caacgacgtt tcatgcggac ctccattctc gaatcctgca ggagtgtcga
    16321 gttcgactgc acagcattct tctcgccggc tatcctacca tgcgattttt ttctggagca
    16381 acggcagtgg tccggcaaga atgccttgcc taccggatgg attgcaggtg ttcaacaata
    16441 ccaacagcaa ggcagcgggg agcgcggagc cgaagcatac tcttttcggt atgttgagta
    16501 tccaagcgag gcgagaacgt gagccggtga aaggcgcgtc tcggcgcgcg ggggctgggt
    16561 gggtgagaaa gcggtcgttt tcaacacctg ctagtgacgt tcgagcaagg gggtgatcaa
    16621 gcggggcagg tcgacgacgt ccctgccaaa atgcaagcga tagcaggaag cccgctgcac
    16681 cagcttcgaa agaacctgaa actcctgtgc cgccgtgtcg gcatcgtgcc tgtaggcctc
    16741 acgtcgaacg agcgcttcga gcgccaggct tttggccagg ggctcgaggc aactgtgaga
    16801 catgttcgtg acttgaagat acagaatcat ggccggctcg catgagcgtc cccgggaccc
    16861 cggatacaca tcttccgtat gaaatgattt tcggtatgca ccctgccgta agagcccaag
    16921 cccggcggtc cgtaactcgg gaaacatctc gatggtgtgg tccgtcacat cgatgttcac
    16981 agaagccccc agcaatagtt gcattggacc gtgatcctgc aacagcgaaa tatcgtcccc
    17041 caggtagcgg tatccggaac ggagcaaaga cagcatggcg gtcgtctttc cgcatccatt
    17101 gggcccgtga agcagcaccc ctgtgccccg aaattctagc gccgccgcag gcagtggaaa
    17161 tgtattcttg ctcttcagca actcgatcat cgtgtaatgg aaaaatgttt ccagtcggtc
    17221 aggccccatc gtctcgggac aataaaaatg gccggatgcc gcactgcggt ttcggtctat
    17281 tagcagtacg ccgtaatccc ggacatccac caccagacgc tccccgtcgc gatacacgtc
    17341 gcattgccag agagcgcgga gtgaattgcc cagcgctggc atggtaccgg aaaacactcg
    17401 tctggcgccg gccgagagag agacgggaac ggcttcgcgg ctgcttacct gctcaaagtg
    17461 ggcggtcaat gagacgtccc ctttcacgtc cccacagtcg acagtgcgaa tccatgcggc
    17521 gagtgaggac gacaaggacg ccgaggagct gcagaagcgg aaagtgatgc catgaaatga
    17581 gaaccggtat tcacgtgaat cgaccgtggt cgtactctcg gctctgagac ccatcacaag
    17641 ctgcgacacg tagcctcctt ccattggact ctaccggcct ggcccatccg atcgatgaag
    17701 aagcccgtca tggctccatt gcacgacaca ggcgatcatc tgttcacgca ggtcaccaac
    17761 atgcgattcc aatcgtgcag agaccacatg atggatctgc tcgagagtag ccgttcccgt
    17821 acagtgctcc cacagggcgg tcccgaccgc gttgagccgg aaggattggc ccgttaccag
    17881 atctaacagc acagtctcac cctccagaac cacgccctgg acattcggat ttttcgtcgg
    17941 cgcgaggtgc aagattctgc tccaatccgg cttgtggtct ccatcgctcg agaggactgg
    18001 tatccagttt tgggggcctt ctccctccat gaacggcaac aggggtggcg tcgacatatg
    18061 cctgggcggc ctgcaccaca tactgattct ctccgacggt tctcgacggc ccagactaat
    18121 cctctagatg ctatattcac agctccctaa aagctgggcg caatccccta ctttcgtaga
    18181 ggcccatgtg ctgagaaaga aaaatgaggc tggcgactct cttacgagga gcgctcgaaa
    18241 aggccgagtt ggaattcttc agcccgggta aaggaaatgg ggtactgctc ggtgaaacaa
    18301 gcgttgcagt aatgatcggg ggcaccgggg gcggatttaa gcatcccgtc gagactgaga
    18361 taggctaagc tgtccgccgt gatgtacttg cgtatttcct cggcggaatg actggaagcg
    18421 atgagttcct ttttcgtcgg cgtgtcgatc ccgtagaagc atggtgaaat gatcggtgga
    18481 gaactgatcc gcatgtgcac ttccttcgcg ccggcctggc ggatcatctt cacaatcttg
    18541 cggctggtgg tcccccgtac cagggaatcg tcaacgacga cgacccgctt gccgtggagc
    18601 acctccggca ccgcattcag cttgaccttg acgccgaaat gcctgatgga ctgctccggt
    18661 tcgatgaacg tgcgtccaac gtaatggttt cggattaatc cggtctcaaa cggaatgccc
    18721 gccccttcgc tataacctaa cgcggccggc acgccggagt ccggcaccgg tatcacgagg
    18781 tcagccggca cccatgactc ctgagccagt tgacgtccca acgctttccg agtcgcatac
    18841 accgcgttgg cgccgaaaat tttgctatca ggcctggcga aatagacgta ctcgaagatg
    18901 cacattgccg gctgcaccgc ggaaaacggt ttgtaactct tcacaccatt ttcattaagg
    18961 acgacaagtt cacccggctc gatttcccgg acgatctccg catccaataa atcgaatgca
    19021 caggtctccg aggcaaccaa gtacgtgtcc cgcagtcgcc ccagcgatag cggcctgaag
    19081 ccatgtggat cccgcgccgc gatgattccc tcgtccgtca gcagtacaac ggagaatgcg
    19141 ccgcggactt ggctcagagc gtcgatgacg cgggccaaca acgtgtcggc tcgtgaatgg
    19201 gcgatgaggt gaatgatgac ttcgctgtct gaggttgatt ggaagatcgc gccgtatgct
    19261 tcgagttcgt tgcgaagtac ctgggcattg atcagattgc cgttgtgcgc caaggcgaga
    19321 ttgccgaacg cgaaattcac cgtaagcggc tgaacgttct tgaggtcctg gccgccggcc
    19381 gtggaatatc gattgtggcc aatcgccatc gatccggtga gatttttgag tgtctgcgtg
    19441 ttgtagatgt cggccacgag gcccatgccc ttgtgcacat gaaaatggtc gccgtccgca
    19501 gacacgatgc cggaggcctc ttgcccgcgg tgttgcagag cgtagagacc aagatacgtc
    19561 agattcgccg cttccttgtg cccgtaaatg ccgaacacgg cgcactcgtc gtgaaactta
    19621 tcggacccga cgatgggaag ttccttacgc ataggcaacg ctattcatgg cttaactgtt
    19681 cttcaagcgc attggcccaa cggtcccgga cctggtcgat cggaagatcg atgcgacagc
    19741 cctcgctcaa tcggcccttc ggcacgtcaa tgacgaatcg atctccgccg acggtgccaa
    19801 tgcgccctgc cggtattccc gcatctgctg cacgactcaa cagggtatcc acggtatccg
    19861 gcctgataga gaggatgacg cgagacggac tttcaccgaa caagagagca tcccgccgta
    19921 acgaaccgag ctccaatttt accacagcgc cccgccttac gtccggaccc gacacgcagc
    19981 tttccgccaa cgcaacagca agacccccgt ccgagcagtc gtgtgcagac tcaacgagtc
    20041 cttcacgaat tacttttaag acaaagtcct gcaacgcctt ttcggtttcg aggttcaaaa
    20101 agggaggaga gccctgttcc cgatgctgca tgaccttaag gtactcactg ccgcccagat
    20161 catccctcgt cttaccgagt aaaataatga catcttcatc atggcggaac cattgggtca
    20221 tggcctgctc ggccttatcg atcaacccga ccatgccgag catcggcgtc ggataaattg
    20281 agaggccgtt cgtctcgtta taaaaactca cgttcccgct cacgatggga atttggaaat
    20341 gctcgcacgc gtcctttaac ccttcgatcg ccagtacgaa ctgccacatg atgtccggcc
    20401 gctcaggatt cccgaaattg agacaatccg tcagcccgat cggctcagcg ccggaacaaa
    20461 ccagattgcg cgcggcctcg gcaacagcca ggcgggttcc ttcatacgga tgcaacagac
    20521 aatagcggcc attgcagtcc actgtcatgg ccaccgcttt gttcgttccc ttgatgcgga
    20581 cgacggcggc atcggaaccg ggccggacca gggtattcgt ccgtaccatg tggtcatact
    20641 gctcatacac ccaccgctta ctcgcgatcg tcggagaagc caatacggtc aacaaagccg
    20701 cattcgcgtc cttcacgtca ggcatcgaat cgtaggtcag gttggtcagc atatcctgat
    20761 acgccggtgg agagtatggc cgctcatatc tcggcccgtc gtccgccaac gatttagcgg
    20821 ggatctctgc cacgaccttt ccttggtcga tgacacgcaa gagaccgtcg ctggtcactt
    20881 taccgacaac cgccacatcg aggtcccact ttcgacaaat ccggatgcac tcttcctctt
    20941 ttccggcttg tgcaaccatc aacatgcgct cttgcgattc ggacagcatc aattcatacg
    21001 gagtcatgcc ctgttcacgc cgcggcacat gagtgagatc caattccatg ccgttgccgg
    21061 cgcgcgacgc catctcacag gacgagctcg tcagaccggc ggctcccatg tcctggatac
    21121 cgacaaggag atcattcgcc atcaattcca gacaggcttc cagaagtaat ttttcttgaa
    21181 agggatcacc cacttgcacc gtcggccgtt tctggtcgga ttcgtcgtcg aaggcgtctg
    21241 acgccatcgt ggccccgtga atgccgtctc gacctgtctt tgatccgaaa taaatgaccg
    21301 gattcccgac ccctgcagcg gtaccgcgga agattttatc cttgtgcgca ataccgaggc
    21361 agaaggcgtt cacgagcggg ttcagcgagt agatgtcgtc gaagcgcact tccccgccga
    21421 ccgtgggcac tcccatgcag ttcccataac cggcaatgcc cgcaacaaca cccctcatga
    21481 gatggcggtt cttggcgctg tccagactac cgaaacggag ggagtcgaga agcgcaatcg
    21541 gcctggcgcc catcgtgaac acatcacgga ggattccgcc gacgcccgtg gctgcaccct
    21601 gatacggctc aatgaaggac ggatgattgt gcgactccat tttaaagacc acgcacagcc
    21661 cgtcaccaat atcgacagca cccgcatttt cgccaggccc ctgcacgacg cgcggtcctg
    21721 tcgtaggcag cttcttcaga tgcagcctgg aacttttata cgagcaatgt tcggaccaca
    21781 tgaccgagaa catgcccagt tcagtcagat tgggctcgcg gcccaaaatg tcgacgatct
    21841 tcttgaactc gtcctcactc agattgtgct gtgcaagaag gtctttgatg tcagccgtgt
    21901 tcgtgtgcat aggtcctata cattacagtg acaacccttg gccctcaaaa ggtcgagctg
    21961 ctcttttctg gtgtgaaccg gccgtcacgc tcctaccatc tttaacggcc gcttcttcaa
    22021 cgattccacc atcgacgcaa gaatcattcg gccgtccgca ttccccaaca gctcctctgc
    22081 ggagcgctcg ggatggggca tcatgccaag tacatttccc tcggcgctaa gaatgccggc
    22141 gatattgtcg agcgagccgt tgggattggc ttcaggtgtc acctgcccct caggggtgca
    22201 ataccgaaac acgatttgag cgttggcctg taatccggcc agcgtaaccg ggtcggtata
    22261 atagttccct tccgcatggg caattggaat cttcagtacc tggccgggct tgcacgcgcc
    22321 cgtgaacggc gtggccgcgt tctccacacg cacatgcacg tccttacaga caaagtggag
    22381 cgaacggttc ctcaacatgg cgccgggcaa cagtccggct tcgaggagaa tctggaaccc
    22441 gttgcagaca ccgatgacca agccgccgct tttggcaaag tctttgattg cgggcatgac
    22501 cggtgagaat cgggcaatgg cccccgtacg gagatagtct ccatatgaaa acccccccgg
    22561 caaaatgaca ccgtcgagac cggccaagga cgtttcctta tgccagacca tcgtcacttt
    22621 ctggtccagc acgtccttga agacatgctc gcaatcgtga tcacaattcg agcccgggaa
    22681 tacgatcacg ccgatattca tgtcgagacc aattcgtacc ggtattcttc gatgatggtg
    22741 ttggccagaa gcttctcgca catcgacttc acctgagctt cagccttcgc cttatccgcc
    22801 tcgctcacat ccaattccat atacttgccg acccgcacgt tcgcggcgtt cttaaagcca
    22861 agagaatcga gcgcatgctc gatcgccttg ccttgcggat ccagaatgcc ttgcttcaac
    22921 gttacatgaa tcttcgcctt cacgatttag tctcctgctc acccgattgc ttgtttaaac
    22981 cgtcaacata tcgtcgaatc ttgaagatca ctgcgactgt ggcgatcccc cccacggcaa
    23041 gcgcggcaaa agcccagcgc cagggtgaat cgtccaaccg atacgccatc atcgccacga
    23101 ctcccgccca gaccagcacc gccgccacca agccataatt caaaaacgcc ttcagcatcg
    23161 ctttatcctc ctcccccttc gccctgtgga ggaaggatgg gactgacttc ctggcgacgt
    23221 gcacgtaggc tcgggctacc gcacactctg aaccggcacg gtccgcaacg cggatgcccg
    23281 acgctgttcg acgttccgtt taagccgtgc ttgaaacgga acaagttgcg gcgggcaagt
    23341 ggcgtgaaga gttcgcgcgg tcgagccgaa gtgcagaagc ccggaagtca gaccagccag
    23401 catcctttga cctacccgca tacccgcttc agcacctcct gatacgcctc ttctatcttg
    23461 cccaagtcct tccgaaaccg gtccttatcc atcgattctc tcgtcgcctg atcccaaaac
    23521 cggcacgtgt caggggaaat ctcatcggcc agaataaggc ggccgttatg gacgccaaac
    23581 tcgagcttga aatcgacaag gaccatccga cgttctgcaa agaacggctg cagcacgcta
    23641 tttactttta atgcgagccg cttgatctct gccatctgtt ccggcgtcgc gagcttgagc
    23701 aggcggacgt gttcatcggc gatcaacgga tcccccaagg cgtcgttctt gtagtaccac
    23761 tcgacgatgg gcggttgaat cccctcacct tctttgagtc ccaatcgctt ggcaagactg
    23821 cctgcgatca catttcgcac caccacttcg accggcacga tcgtcacttt cttcgtgagc
    23881 atttctcgat cgctctttcg ctcaacgaaa tgagtggcca ccccgttctg ctccaggagc
    23941 cggaacaatc gctccgagac cttattgttg atgatgccct tttcggcgat ggttccgcgc
    24001 ttctgcgcgt tgaacgccgt cgcgtcgtcc ttgaaatact gcagcacttc atccggatgt
    24061 ccggttgtga atattttttt ggctttgccc tcgtatatca gagtgcctgc cgtcatgttt
    24121 gtctccgact cttactgcgc caggatttcg atgatttcat ccagaccctt ggccgtcccc
    24181 agccaggtcg gatgaccaaa gcgcctggtg tattgaaact ctttcacttt tcccaacgat
    24241 agaatcaagc cgtgaaagtc ctcgagcttg gaggtttcga aataggtgat gaagtccacg
    24301 tcgtccagac ccgtcgaatg gtagagctta cgcttgacgg tcttatgata gggtaaagag
    24361 gcctcggtgt gctccttcat catcgccgcc cgcttgtcct gatccagcgc ccaccagtcg
    24421 gcgctctttc gaatcggaat gatgaccaca tagggtttgg gacctggatc actcgtgacc
    24481 ttgaggtctg ttttcaattg gtcgggaaaa cccggcacgt aattggcctt tttggtcaag
    24541 ccatgcatca cgccggcggc cttcaagtgt ttaccgaaca cgctgctctg taagtcaacg
    24601 agaaatttct gcgtatcgcg aagttcggtt gcgtgcgtcc taaacaggat atcggcatga
    24661 tcggatagcc cctccatgag atacaaatcg atggcaatct tttcccgatg ctgctcgaca
    24721 acccctttga acacggtcag gtgcgcgata cgggtcgctt cgtcctcctt cgcccattct
    24781 tcgtcgaaac tgtacgcggc aaaggtgccg tatacgcccg gctcggtcaa caacttttcc
    24841 agatcggctg ccgcaaaaca gagtacggcc cataatgacg cactcatcgt aatcagactc
    24901 acgaagccgg cgcgctgcgc tgtcatgtcc ttcctttctg aggggcatgc cccacgctgc
    24961 ggaacactct cttgaagatc tcatcgacat gccgcagata atacttgggg ttaaaacagg
    25021 cggcgatttc gcttttctta agatgttgtg cgatgaacgg gtccttgctg accagttcct
    25081 gcaggtttcc cgacccgttc catgaagcca tggcgttgcg ctgcacggct tcatacgatt
    25141 cctttcgctg cgcacccttg tcaatgagcg ccagcagtag gcgttgggag tagaccaatc
    25201 cgccggtcag ctcgagatta cgcttcatgt tggcgggata gacgaccaga tgtttgatca
    25261 ggtccgtgac tttcgccagc atgtagtcaa cgaggatggt gctgtccggc atgatgacgc
    25321 gctcgaccga ggaatggctg atgtcccgtt cgtgccacag agccacgttt tccatggctg
    25381 cgaaactatt ggcccgcacc agccgcgcca atccgcagag attctccgac acgatcggat
    25441 tgcgtttgtg cggcatggcg gacgagccct tttgtcctgc cgaaaaatat tcttcggcct
    25501 cgagcacctc agtccgttga agatgccgga tctcagtcgc gatcttttcg attgtggccg
    25561 caagcagcgc cagagatgtc gcataggcag catggcggtc ccgttgcacg acttggttgg
    25621 aaaccggatc cacggtgagg ccgagcttgg cacacacgta ggcttcgata tccggtccct
    25681 gatgtgcaaa cgtccccatc gcgccggaca gcttgccgac tgcgatctca cggcgcacct
    25741 gacgcagccg ggccaaatgt cggccgactt cttcgtgcca aatggcgagc ttgaacccga
    25801 acgagatcgg ctccccatgg ataccatgtg aacgccccac catcacctgg ctcttgtagc
    25861 gaaaagcctg ccgcttcaac accgccagca aaccctccac cccttccaaa atgagatcaa
    25921 gggcttcggt catctggatg gccagcgacg tatcgacgat atcggatgag gtcaacccca
    25981 tgtgcaaaaa ccgatgctcc ggtcctacag tgtccgccag tgattcgagg aacgcgatca
    26041 cgtcgtgttt cgtgactttc tcgatttcgg cgatccggga cacattgatc ttggcctttt
    26101 tcctgatttt tgcagacgtg ccgcggggcg cctgcccggc cttttcgaat gctgcacagg
    26161 cctgcagttc cacctccagc cagatctcat acttgtgctt gagatcccag atggctttca
    26221 tctgtggacg cgtatagcgg tcaatcattg gacgtccggg cgctcactcc tcacgcccgc
    26281 cctgaggctg acgaacggcc ttttgtctct tgagtgccaa cttcccttcc gttgtcagca
    26341 ccttgtccaa aatgccgttc acaaatttcg cggcttcctc atcaccgaaa tccttcgcca
    26401 gctcgatcgc ttcgttgacg gtgacttttg ccggcacttc atccagccac aacagttcat
    26461 aggctccgag ccggaggata ttgcgatcga cgatctgcat gcggctgacc ttccaattca
    26521 tcgcatattt gccgatgagc gcatcgagat cgtttttgtg tccaagcacg ccgctgacga
    26581 gctgttcggc gaacgatctg acgtcttctg tggcttggaa ctgattccag aactcgtcca
    26641 gccagacgcc gggtttgccg tggatgtcgt attgaaacaa gatttgcagc gcgcgctcgc
    26701 gcgactgatg gcgagatccc ataatcaggt cttgtgcttt cgtgccggac gcctggctgc
    26761 cccgtttttt ccggcggtct cctttggcgg agctgttgtc tcagtgcccc gtatcatccg
    26821 catgaccgtc gccatctcaa tcgccgactt cgccgcctcg ccaccccgat tgaacttggt
    26881 cggctccgct cgcacgatcg cctgggccac ggtgtcggtc gtcagcacac caaagataat
    26941 cggcacgtcc gcatccaagg ccgcctgacc gatgccgcgg ctggcctctg cactgatata
    27001 gtcgaaatga ggtgtatcgc ctcgaatgac ggcgccgagg cagatcacgg cgtcgaaccg
    27061 tttggttccc gccagagtcc tcgcgacaag cgggatttca aaggcacccg gcacacgcac
    27121 gacttcgata tcttcgggca ccacgccgtg tttggtcagc ccatcaatac aactgctcaa
    27181 cagcttactc gtgacgaatt tattgaattt cgcgacgacg atcccgaact tcagcccagt
    27241 cgcgttatgt gagcctttgc gaagtttcat gctaatgagg ttacgtttgc gatctcgatt
    27301 gatcaggtca gcagtaaagt aactgcacac taccacttgc cgtcacacct ttttcaggat
    27361 gtgcccgagc ttcttttttt tcgtcttgag atatttgaca ttgcccgcat gcggctgaat
    27421 ttcaatcggc acgcgctcaa cgactttcaa gccgtacccc tcgattccga caatcttccg
    27481 cgggttgttg gtaatcaact tgatttggtg caggccgaga tttgcgagga tctgcgcgcc
    27541 gaccccgtaa tcccgcagat cggccttgaa gcctagcttg aggttggcct ccaccgtgtc
    27601 gctcccctta tcttggagtc cgtatgccct gattttgttc agcagcccga ttccccgtcc
    27661 ctcctggttc aggtagagga gcacaccccg gccttccttc tggatgactt ccatggccgc
    27721 gtgcagttga tcccggcaat cgcaacgcag agatcccaac gcgtcggcgg tgagacagcc
    27781 ggagtgcacc cggaccagcg tcggcgctcc gttatccacc cgccccttga ccagcgcaat
    27841 atgcgtgatg tgatcaatct cattttcaaa ggcgacggca tcgaattccc cgaagatcgt
    27901 cggcagcctg gcgctcgcca tgcgcttgat aaaggtttcc ctgcgcatcc ggtattcgat
    27961 cagtgccttg accgttacca tcttgagctt gtgtgtcttg gcaaacttgg tcagctcagg
    28021 cacccgtgcc attgtcccgt cctcgttcat gatctcgcag atgacgcctg cggggaggag
    28081 accggccaac cgcgcgagat cgacggaccc ttccgtttgc cctgcccgtc gcagcacacc
    28141 gccccgttgg gccttcaatg gaaaaatatg gccgggtcgg gcgaggtcgc tcggcttcga
    28201 cttgggatca attgccacat gaatggtcgt ggcacggtct gcggcagaaa taccggtcgt
    28261 gatgtccttg cgagcgtcga tcgagacggt gaaggcggtg ccgaatgccg cggtattttc
    28321 aacggcttgc tgtggcaact gcaattcttc gacgcgctcg ggcgtcagcg cgaggcagat
    28381 cagcccgcgc gcgtgcctgg ccataaaatt gatggcgtga ggcgtaacct tttctgcagc
    28441 aatgacaaga tcgccttcgt tctcccggtc ttcatcgtcg accaagatga cgaacctgcc
    28501 cttcttgatg tcccggattg cgtcttcgat gtcgtcaaaa ggggtttgca tactttaact
    28561 gacaggcgtg gcctttccgg tcgttaagga cgatcaattt atcattgaac atggaccatt
    28621 aacatgggtt tttttaagcc gcttcctcga gtttacgtgt ttatgacgaa gagccagtga
    28681 ccgcctcatg gcgtttgcgc aacgcagcga tcacggtgcc ccatgcacac atgacaaagc
    28741 cggcgaagag cca
//
LOCUS       DASTQY010000280         4962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1301355, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000280 DASTQY010000000
VERSION     DASTQY010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301355"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="VFR82_09320"
     CDS             <1..1262
                     /locus_tag="VFR82_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501838.1"
                     /translation="PGFAGAIAGDIAPQFSLQQLTNLGKGNYLFKLPTNVQLDFFKQI
                     TDAKTLAAPKVRVVNNKKAEINIGDKQPILLSTTNVLPGQAATGAVPTTSTVTSIEFR
                     DTGVKLTVEPVIHLGNELSLKMKIEVIRLGEQVTLQANPPITQFKFGNRSAETMLNVK
                     DGETIVLGGLLQEEDRRTRVTIPWLGDLPLIGNLISSFKTQRVTTEVILTLTPHIVQA
                     MTPPSLSRQAFWSGTDSNYSNSTMFTTQQHRATPTRMKQTQVSSLAPSPMSQRGIAAK
                     APSGSLTKMLTVGPVASIKPNDSIVEVGKEFKIAIQDDRLRPEKGGLFHLQYDPNVLD
                     LKNLIDAEQIQVDPAELPPSAVAEAIVAFRVGASAPRASTGGRTVTARFRAKAPGVSP
                     IRVALVNANGDLAADASPEGRGIVRVR"
     gene            1259..1765
                     /locus_tag="VFR82_09325"
     CDS             1259..1765
                     /locus_tag="VFR82_09325"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU4501839.1"
                     /translation="MREEGVTLLELLVTLTIVMILASVAMPLSRVSTKRSHEIELRQH
                     LRIMRAAIDTFKIEWNRDGDVLIGPLCVKNKLSCKDVTSIYGYPKSLEMLLGVKLTSE
                     EATVRGTTTRRYLRSLPLDPLTGEANWQFRCYKDAPNASSWCGDDVYDVMTVSKETAL
                     DGTKYRDW"
     gene            1740..2141
                     /locus_tag="VFR82_09330"
     CDS             1740..2141
                     /locus_tag="VFR82_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU4501840.1"
                     /translation="MERNTGIGEFRLAGAPGFTLIELLIVVSIIGILATLAAPSYQTS
                     VVKAKEAALRQDLFTLRDVLDQHRADQGKYPASLGALVSAGYLRAIPKDPFTGSTTSW
                     QEMTDAMEGGTFDVFSGSELVGTNGTPYNRW"
     gene            2144..2905
                     /locus_tag="VFR82_09335"
     CDS             2144..2905
                     /locus_tag="VFR82_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU4501841.1"
                     /translation="MHLSPYDTVLHGLRGNDVRTDRSAVRAITLCVLAVVALAASACG
                     ELEPMIEPEMVDLQLTLDTLRTQVRDAQRNLAEVRTELESRRQELADAQVARAQLEGR
                     VREAERRVTEARQVIELQREELIAAKTERERVFRSSSQQLKQLQRRGAKSGGLDSAER
                     GASAVPTDATLPWFFTNPSRSATPAINGLSSKGTATAVAVRRQPPLTEIVVKQGDTLY
                     SLARKYRMTLSRLRSLNHVSDDQIIVGQILWVTEN"
     gene            2956..4167
                     /locus_tag="VFR82_09340"
     CDS             2956..4167
                     /locus_tag="VFR82_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU4501842.1"
                     /translation="MAVFAYRVARPDGSTLEGHIEGDEEPLVRAKLEGQGFLIFQLKR
                     RGVASSRPAIDLSGFRGLPISEFLVFNQELLALVKAGLPVLRVWDLLIERARHRGFQQ
                     ALRMVRQDIRGGASASEALTRHPGYFSELYVATIRAGEQSGNLVEVLKRFIGYLKLMI
                     GLRQKVGKALAYPAFLVLTGIAVVGFLLSYVMPTFISVYAETAKSLPFATQMLIDVVS
                     GSRLYVIPVAALLVGLLLLGRAYYSTPHGQIRVDQLLLRAPVLGAVFVKHHTIQLART
                     LGTILAGGTPLVEALHITKSAVSNRYIAVGLTEAVSEIREGTTLASALERPKVFPKLA
                     IEMLSVGEETGSLEAMLHDVAEFYEADLDLRLTQLTTWIEPVLLLVMGILVGGIVIVM
                     YLPVFQMAGNV"
     gene            4235..>4962
                     /locus_tag="VFR82_09345"
     CDS             4235..>4962
                     /locus_tag="VFR82_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU4501843.1"
                     /translation="MTRPTLADVLVAQGVVTQSTVEHTLNRLGGVSADLGATLLGEGA
                     LSEDQLARALASQFGLPFDPLVDFRVDQSFYERMSVKLMQRHPFVPIAECEGRLKVAV
                     PDPQDLLGIDELELLLGRELELVVSPKSAILAALEHSEGSSQALRELEAEYRSVLVKE
                     DERGEEILTLDRTGEDQSPAVKLLDSIFLSAMQRRASDIHIEAADRSTRVKFRVDGIL
                     VQAMEPLDIRLHAPLVSRLKVMSDL"
BASE COUNT         1051 a         1335 c         1520 g         1056 t
ORIGIN      
        1 cgccgggctt cgctggagct attgccggcg atattgctcc tcagttttcg ctgcagcagc
       61 tcacgaattt ggggaaaggg aactatctct tcaagctccc aaccaacgtg cagctggatt
      121 tcttcaagca gatcacggat gcgaagacgt tggcggctcc caaagttcgc gtggtgaaca
      181 ataagaaggc cgaaatcaac atcggggaca agcagcctat tctcctctcg actaccaatg
      241 tgctccctgg tcaggccgcc accggcgcgg tacccacgac ctccaccgtg acatccattg
      301 aattccgaga caccggcgtc aagttgacgg tggagcccgt tatccacctg ggaaatgaac
      361 tgtcgctcaa gatgaaaatc gaggtcattc gtctgggcga gcaggtgacg ctgcaggcca
      421 atccgccaat cacccaattc aagtttggta atcgctcggc tgaaacgatg ttgaatgtca
      481 aagacggtga aactatcgtg ctgggcggat tgcttcagga ggaagaccgc cgcacgcgcg
      541 tcacaatccc atggctcggt gatctcccct taatcggcaa tctgatcagc tcgttcaaga
      601 cccagcgggt cacgaccgag gtgattttga cgttgacccc acatatcgtc caggccatga
      661 cccctccaag tttgagtcga caggctttct ggtctgggac tgactcaaat tactcgaatt
      721 cgaccatgtt cacgacgcaa caacacaggg ctacgccgac aaggatgaag caaacgcagg
      781 tgtcgtcgct tgcgccttca cccatgtcgc aacgaggaat tgccgcgaag gcgccttccg
      841 gttccctgac aaagatgttg acggtgggtc cggtcgcttc aattaagccc aatgattcaa
      901 tcgtagaggt tggaaaggaa ttcaagattg ccattcaaga cgacagattg cggccagaga
      961 agggggggct cttccacctt cagtatgatc ccaacgtgct ggacctgaaa aacttgatcg
     1021 atgcagaaca gattcaggtt gacccggcag aactgcctcc ctcggccgtt gccgaagcca
     1081 ttgtcgcgtt tcgggtcggc gcctctgctc cacgtgcctc gactggcgga agaacagtca
     1141 ccgcaaggtt tagggcaaag gcgcccggcg tctctcccat tcgtgttgct ctagtgaatg
     1201 cgaacggaga cctggccgct gatgcgtcac cggaggggag aggcattgtg agggtgcggt
     1261 gagagaggaa ggagttacgc tccttgagct tctcgttacg ttaacaattg tcatgatcct
     1321 ggcctcggta gcgatgccat tgagccgggt ctcgactaaa cgatcacacg agatcgagtt
     1381 gcggcagcat ctacggatca tgcgggcggc catcgacacg ttcaagatcg aatggaatcg
     1441 ggacggcgac gtcctcattg gtccgttatg cgtgaaaaac aagctgtcgt gcaaagatgt
     1501 cacgagcatc tatggctatc caaaatcgct ggagatgctt ttgggcgtca aactgacgag
     1561 cgaggaagcc accgtacgag gcacgacgac caggcggtac cttcgaagcc tgccccttga
     1621 cccgttgacc ggtgaggcga attggcaatt ccgctgttat aaggacgcgc ccaatgcatc
     1681 gagttggtgc ggggacgacg tctacgacgt catgactgtc agtaaggaaa cggcgttgga
     1741 tggaacgaaa taccgggatt ggtgaattcc gtctcgccgg ggcgccaggt ttcacgttga
     1801 tcgagctgct gattgtcgtg tcgatcatcg gtatcttggc gacgttggcc gctccgagtt
     1861 atcagacgtc cgtggtcaaa gccaaggagg cggcattgcg gcaagacctc ttcacgctgc
     1921 gcgatgtgct ggatcaacat cgggcggacc aggggaaata tcctgcttct ctgggagcgc
     1981 ttgtaagcgc cggttacctg cgggcgattc ccaaggaccc cttcacgggc tccactacat
     2041 cgtggcagga aatgaccgat gcgatggaag gcggaacctt tgatgtgttt tcaggctcag
     2101 aattagtcgg gacgaacgga accccgtaca accgctggtg agcatgcacc tgtctcctta
     2161 cgatacagtg ctccatggcc tgagagggaa tgacgtccga accgaccgct ccgcagttcg
     2221 tgcgattacg ctttgtgtcc tcgctgtggt cgctcttgcc gccagcgcat gtggcgagtt
     2281 ggagccgatg attgaaccgg agatggtaga tctccagctc accttggata cgctgagaac
     2341 ccaggtgcgt gacgcccaac ggaatttggc cgaagtgcgg actgaactgg agtcgcgacg
     2401 ccaagagttg gctgatgccc aggtggcgcg tgcccaactt gaaggtcggg tgcgagaggc
     2461 ggaacgtcgg gtcacggaag cccgtcaggt catcgaactg cagcgagaag aactgatcgc
     2521 cgctaaaaca gagcgggagc gtgtcttccg cagtagctcg cagcagctga agcaactcca
     2581 acgaagaggg gcaaaatccg gcggtctgga cagtgccgaa aggggtgcct cggcagttcc
     2641 aactgacgca acgttgccgt ggtttttcac gaatccgtca cggtcggcga caccggcgat
     2701 taacgggcta tcgtcgaagg gaacggccac ggcggtggcc gtgaggcggc aacctcccct
     2761 cacagagatc gtggtaaagc agggtgacac gctgtatagc cttgcgcgca aatatcggat
     2821 gacactcagc cgcctgcggt cgctcaatca cgtgagcgac gaccagatca tcgtcggtca
     2881 gatcctgtgg gtgacggaga attgaccgtg cctcagggct gcgatcggca agcccctcgt
     2941 tcatgagtat ttcttatggc tgtattcgcg taccgcgttg cgcgtcctga tggatcgacg
     3001 ctcgaaggcc acatcgaagg cgatgaggag ccgctcgttc gggcgaagct cgaggggcag
     3061 ggatttctca tctttcaact caagcgtcgg ggcgtcgcct ccagccggcc ggcgattgat
     3121 ctcagcggat ttcgaggtct tccaatcagc gagtttctgg tcttcaacca ggaactgttg
     3181 gcgctggtga aggcaggcct tccggtcctc cgcgtgtggg atctgttgat cgagcgcgcg
     3241 cgccatcggg gttttcagca ggcgttgcga atggtgcgtc aagacatccg cggcggagca
     3301 tcggcttccg aagccctcac caggcacccg ggatattttt cggagctgta tgtcgccacg
     3361 atacgggcgg gggagcagtc gggaaatttg gtggaagtgt tgaaacggtt catcggctat
     3421 ttgaagttga tgatcggcct tcgccagaaa gtgggcaagg cactcgccta tccggccttc
     3481 ttggtgttga ccggcattgc cgtcgtcgga tttctgttga gttacgtcat gccgaccttc
     3541 atttccgtct atgcagaaac cgccaaatca ttgcccttcg cgacccaaat gttgatcgat
     3601 gtggtgtcag gctccagact gtacgtcata cccgtcgccg cgctgcttgt cggtctgctg
     3661 ttgctcgggc gagcctacta ttcaacacct catggccaaa tccgggtgga tcaattgctg
     3721 ctgcgtgcac ccgtgctggg agccgtcttt gtcaagcatc acaccattca gttagcgcga
     3781 acattgggga cgatccttgc cggagggacg cctctcgtcg aggcgttgca tatcacgaaa
     3841 agcgcggtct ccaaccgata cattgcagtg ggattgaccg aggcagtgag tgagattcgt
     3901 gagggcacga cgctcgcctc ggcactggag cgaccaaagg tttttccgaa gctcgccatc
     3961 gaaatgttgt ccgtcggcga ggaaaccggc tcgctcgaag cgatgttgca cgatgtggcc
     4021 gagttttatg aagccgatct cgaccttcgt ttgacacaac tgacaacgtg gatcgagccg
     4081 gtgctcctgc tggtgatggg aattctagta ggaggaatcg tgattgtgat gtatctgccg
     4141 gtctttcaaa tggcgggaaa cgtgtgagcg attcatgacc tatccgagcg ggcgtcttcc
     4201 gagtcccaag agaggtgcgg tggcagctcg aagcatgacg agaccgacac tggccgatgt
     4261 gctggtggca cagggggtgg tgacacaatc gacggtggag cacaccctga accggctcgg
     4321 cggcgtctcg gcggacttgg gagccacatt gttaggcgaa ggggcgctgt cggaggatca
     4381 gttggctcgt gcactcgcca gccaattcgg actgcccttc gatccgctgg ttgatttcag
     4441 ggtggatcaa tccttctacg agcgcatgtc ggtcaaactc atgcagcggc atccatttgt
     4501 cccgattgca gagtgtgaag gccgcctcaa agtcgcagtt ccggaccccc aggacctgct
     4561 gggcattgat gagctggaat tgttgctggg ccgggagctg gagctggttg tgagcccgaa
     4621 gtcggcgatc cttgctgcat tggaacacag cgaagggtcc agtcaggcgc tgcgggaact
     4681 cgaggcggaa tatcggtccg tgctggtgaa ggaggatgaa cggggagaag aaatcctcac
     4741 gctcgaccgc acgggggagg atcaaagtcc cgccgtcaaa ctgctggatt caatattctt
     4801 aagcgcgatg cagcggcgtg ccagcgatat tcatattgaa gcggccgatc gttcgacgcg
     4861 cgtgaagttc cgcgtggacg gcattctggt ccaggcgatg gagccgctgg acatccggct
     4921 acatgcgcca ctggtttctc gattgaaggt catgtcagat ct
//
LOCUS       DASTQY010000281        11844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1247290, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000281 DASTQY010000000
VERSION     DASTQY010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11844
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247290"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="VFR82_09350"
     CDS             complement(<1..1277)
                     /locus_tag="VFR82_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4501844.1"
                     /translation="MDALRARLQWLMGLRVTFVTLMLGLSLIFQATKGERVDTFYTLI
                     VLTYALTIPSAVFLRTLKTPSALTTFFWIQVGIDFLLETVLVARTGGVESPFAVLYVI
                     TVAVASLIPQRRVGLFAACTCIIVFGVVTNAQLYGFASSWGSVAVGHVSAAEVFQTFG
                     VYALALLVVGLLSGTLADQLYRVDQSLREKEQGLNRLQAFHENIVRSISSGIFTADAT
                     GVLTSFNPAAQELTGYTAGQVVGRAWREIFNWHPNQPSDEATAGALPAMTRFEVECKN
                     ANGNRLVLGMTVSPLYEQREEHGLVGVFKDLTQIRELEAEMRRKEWLANLGEMSAGMA
                     HEIRNPLGALAGAMQMLRKESTADATDRRLMDIAIREATRLDAIITEFLQYARPPELD
                     LAEHNINKILAETMDLVQHEARIRKRITIATSLA"
     gene            complement(1351..2565)
                     /locus_tag="VFR82_09355"
     CDS             complement(1351..2565)
                     /locus_tag="VFR82_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU4501845.1"
                     /translation="MATFAYVGRSRSGAVKKGELTAKTKDEAVDQLRKQSVVVTSLEE
                     KTGKGGLKLSFGSGLTDKDLVVFTRQFGTMINAGLPLVQCLEILSTQSENKVLRETVG
                     EVRGQVEAGSTFSDALRRHPKVFDDLYVNMVHAGEVGGLLDTILTRLAKHIEKAMKLK
                     GQIKSAMIYPSAILGVAGIVITVLMIFVIPIFAKMFEELSGGKVGLPGPTQLVIDISN
                     AFQSFWYIAVGLIIGAVMLVKKYYATPKGRMAIDKFLLKTPIFGDLIRKASVAKFTRT
                     LGTLLSSGVPLLDGLSICAKTSGNKVIEEALLNARVSISGGKTIADPLTQSQVFPKMV
                     THMIAVGESTGALDAMLGKIADFYEDEVDQAVASLTSLLEPMMMVFLGVVIGFIVIAM
                     YLPIFKMASAIG"
     gene            complement(2718..3254)
                     /locus_tag="VFR82_09360"
     CDS             complement(2718..3254)
                     /locus_tag="VFR82_09360"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU4501846.1"
                     /translation="MNIPNSLTILRILLIPVYVGLLIYERFDGAMIVLLVAGVTDALD
                     GTIARAANQRTRLGAFLDPLADKLLLTSGFVTLSAIHLIPSWVTILVVSRDAMLLLGT
                     AVAQFTESNIDIAPTFFGKGATFLQLSYVLLVIFLSSRHLDLSVLNPLVIAMVAFTVS
                     SGCHYLYRGYRSVGASPT"
     gene            3306..3701
                     /locus_tag="VFR82_09365"
     CDS             3306..3701
                     /locus_tag="VFR82_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501847.1"
                     /translation="MSNEVNGLQSAVDRPILQLRLHEEMTMNVLASLFKRDGLVEKHQ
                     LEGVDPSDRYFNRAVLVNRTASGYSAKIMYEALTVEGQSHPTIAAAITELVEKLQSLG
                     FSRLRTRANFKGTKYLAEKETWIEYQDLA"
     gene            complement(3703..4827)
                     /locus_tag="VFR82_09370"
     CDS             complement(3703..4827)
                     /locus_tag="VFR82_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU4501848.1"
                     /translation="MKRPSVIQAAMDQAVRDGVFPGGVLAVRLAGELKSLVVAGCLSS
                     DPDDPVQISTVYDLASLTKPLATVTSILLLVQSGKLDLDAPVKHTLGDLEGAPIGAAT
                     LRDLLQHRSGLPGWRPLYEGLTAKGIAWGRSESVRQTVLHLIGEEPLMYRRGEKSEYS
                     DLGFILLGLTVERASGQPLDEYFYESVAKPLGAQPLCFNPTATDCHPARPAVDVSSIA
                     PTEWDEWRRQLLKGQVHDENAAAMGGVAGHAGLFGTAEAVLAVSGVWLRSYEGQRSML
                     EQRLVREFTRRSSPCSHFSWALGWDTPTAPSSSGSHLSPESFGHLGYTGTSLWIDPRR
                     KLEVLLLTNRVHPTRKNEGIRAFRPLIHDMVFQEIVSGTR"
     gene            complement(4987..5499)
                     /locus_tag="VFR82_09375"
     CDS             complement(4987..5499)
                     /locus_tag="VFR82_09375"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEU4501849.1"
                     /translation="MKITPLDIQQMVFKTGFRGYDKEEVNRFLEEIAETVEELNRENA
                     VQREKITFLEQQLIELKRTEVTLSNTLVSAQSLAEDVKRSAQRESELVIKEAELKAGE
                     MIRQARVEMSNTQRDLSALQKQRLLMVERLRATLRTFERMLEVEEQEAFQDGASSAEH
                     KLEGESSPAL"
     gene            complement(5521..5817)
                     /locus_tag="VFR82_09380"
     CDS             complement(5521..5817)
                     /locus_tag="VFR82_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU4501850.1"
                     /translation="MFVVENFFTALASIVYYLLELYMYVVIARAVISWVNPDPWNPIV
                     QFLEKVTEPALAPIRRMIGWRLGVDVSPIILILILIFLQRFIVPSLHQMATVIG"
     gene            complement(5817..6632)
                     /gene="proC"
                     /locus_tag="VFR82_09385"
     CDS             complement(5817..6632)
                     /gene="proC"
                     /locus_tag="VFR82_09385"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU4501851.1"
                     /translation="MTSLMVKKIAFIGSGHMAEAMIGGLLAAGVCGSDALWASDPVAT
                     RRDRLKSLFSIRVGAENRQAVTWANIVILAVKPQSLPTVLSEIGADLNGVLVISIAAG
                     VTIQSITSQVSPGTRIIRAMPNTPALVREGITALSWGREVTEEDSLVARTIFEAVGRV
                     IPVEERLMDAVTGLSGSGPAYVFLALEALADGGVKMGLPRQTAELLAAQTVLGAARMV
                     LETGTHPATLKDQVASPGGTTIAGLHQLEQGKFRATLMGAVEAATKRSQELAR"
     gene            complement(6625..7335)
                     /locus_tag="VFR82_09390"
     CDS             complement(6625..7335)
                     /locus_tag="VFR82_09390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU4501852.1"
                     /translation="MDAEAGDSIENRVAVAFNQICIATRKAGRADGSVQLVGATKSVP
                     VGDIRRAVRAGLTLAGENRLQEALPKIEALKDTGVIWHFIGRLQRRKVRSVVGIFDLI
                     HSVDSLELAREIDRRAKAAGLRQKILLEVNVGEESSKAGFLGDQVERVLPQLGGLDHV
                     AVCGLMAIPPLMREPERARRYFRALRDLAARLIQLKISRISMDELSMGMSHDYEVAIE
                     EGATLVRLGTALFGERRD"
     gene            complement(7392..8126)
                     /gene="pgeF"
                     /locus_tag="VFR82_09395"
     CDS             complement(7392..8126)
                     /gene="pgeF"
                     /locus_tag="VFR82_09395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEU4501853.1"
                     /translation="MTASVITVPAFGLPHDGVRHFFGTRHHTEAVVYETGVPARTRDV
                     SGQSWMLSVKQVHGTDALIVDRPLTGSDRFQGGWDALITDQPSVTVAVRTADCVPVLV
                     YDRRRKVAAAIHAGWRGALAGIVPKTIQLMISRFSTDEADVRVSIGPSAGSCCYEVDE
                     AVLEPLRRGPTECSSLIKDRRGNRAKLDLKALVKRQATQIGIDREHITTVNLCTICHP
                     HLFFSYRREGRVNGTMLNGITLMPHR"
     gene            complement(8165..9343)
                     /gene="ftsZ"
                     /locus_tag="VFR82_09400"
     CDS             complement(8165..9343)
                     /gene="ftsZ"
                     /locus_tag="VFR82_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU4501854.1"
                     /translation="MFSFQEDCLSPVRIKVIGVGGAGCNAVNTMITSGLARVDFIAAN
                     TDLQALDRSRAAYKVQLGPERTRGLGAGAKPEIGKESALESKDQIRECLEGADMVFVT
                     AGMGGGTGTGAAPIVASIARELGILTVGVVTKPFSYEGHRRMTHADEGIRDLRRHVDT
                     LLVIPNQRLLGIVDKATPLLDAFKVADDVLRQAIQGIADVITTTGHVNVDFADVRTVM
                     SHTGRAVMGMGVSRGTNRAIEAAQKAICSPLLEEGSVEGARGVLLNISGGPNMSLHEI
                     EEAASIIQQTADPEANIIVGQVINPDMGDDLVVTVIATGFERDDQPMPVGVASQARMA
                     RAAQPPFNGVAVADRPHKDLDRPTYLRRMGDHRESMERLAVVADDEWDVPTFLRKQAD
                     "
     gene            complement(9388..10623)
                     /gene="ftsA"
                     /locus_tag="VFR82_09405"
     CDS             complement(9388..10623)
                     /gene="ftsA"
                     /locus_tag="VFR82_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU4501855.1"
                     /translation="MAKRDQILVGLDIGTTKICAIVSEVTEEGTLNIIGVGSSPSRGL
                     RKGVVVDIESTVESIKKAVEEAELMAAVQINSVYTGIAGSHISAENCKGVVALKKSEV
                     TRDDIHRAIESARTLAVIPHERRILHVLPREFMVDGQEGVREPLGLSGNRLEVNVHVI
                     TGAVTSAQNIIKSVNRAGLDVVDIILQPLASSEAVLSQEERDLGVAMVDLGGGTTDLA
                     IFLDGSIRHSAVLPIGGQNLTKDLAIGLLTSQTEAEKIKVQHGVTRTELVQGHETVEV
                     PSVGDRPARTFSRRDIAEILEPRVEEMFELVRREITRAGYEGMLGAGVVVTGGTSLLE
                     GMPDAAEHVLNLPARRGVPTGIGGLRDIVSNPMHATGVGLLLHARRHTEETATVGLRS
                     GRPIGKMLDRMKSWMFEFF"
     gene            complement(10628..11497)
                     /locus_tag="VFR82_09410"
     CDS             complement(10628..11497)
                     /locus_tag="VFR82_09410"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEU4501856.1"
                     /translation="MRLLRRKPPAKAAGHRSNEWKGPRASQSMDHRQRARRIERLTRC
                     RRALRLSGWLGGAVAVCWMIVMLSQQLGPMLHRGLEIKQVSVEGTHQVTKQEVIDRLA
                     LKKGLAQYQVSLSYLAERVRIHPWIKEATVERLPLHELRVTVVERTPAAIVRLESDQV
                     LIDSEGVILARLGTRDEATLPLLTGVDGTLLAQGDLRIKQRIQSAVELARWMAESVDG
                     RIELDVAHPVNLVASARGVRFHFGSDALKDQWERFMKVKAAFRMPALDGRKHEGHDVD
                     LRYDNRVIVRERG"
     gene            complement(11494..>11844)
                     /locus_tag="VFR82_09415"
     CDS             complement(11494..>11844)
                     /locus_tag="VFR82_09415"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU4501857.1"
                     /translation="VEIVAPGGFYDFSAKYEKGKTRYLCPAPLPAAVTKEIGVLALRA
                     FEVLGCAGAARVDFRITPRGRPYVLELNTVPGMTETSLLPMAAVQAGIGYEELTERIL
                     ESALPRMKNKEHAA"
BASE COUNT         2561 a         3612 c         3173 g         2498 t
ORIGIN      
        1 gccaatgagg tcgcgatcgt gatccgtttt ctgatccgcg cttcatgttg tactagatcc
       61 atcgtctcgg caagaatttt gtttatattg tgttcggcca aatcgagctc cggcggcctc
      121 gcgtactgaa gaaactcggt aataatggcg tccagtctcg tcgcctcccg gatggcgatg
      181 tccatgagcc gccggtcggt cgcatcggca gtcgattcct tccgtagcat ctgcatcgcg
      241 ccggccaagg cgcccaatgg atttctgatc tcatgggcca tcccggcgga catttcaccc
      301 aggttggcca gccattcctt gcgccgcatt tctgcttcga gctctcggat ttgcgttaag
      361 tccttgaaca caccgaccaa cccgtgctcc tcccgttgtt cataaagcgg cgagacggtc
      421 atgccgagca cgagacggtt gccgttcgca ttcttacact ccacctcaaa ccgcgtcatg
      481 gcaggcagcg ccccggcggt tgcctcgtcg gacggctgat tcggatgcca gttgaaaatt
      541 tctcgccaag cccgcccgac gacctgacca gctgtgtagc cggtcagttc ttgcgccgcg
      601 ggattgaaag acgtgaggac gcctgtggcg tcggcggtaa agatcccgct gctgatgctc
      661 cggacgatgt tttcgtgaaa ggcctgcagc cggttcaacc cttgttcttt ttcgcggagc
      721 gattgatcga cgcgatagag ttgatcggcc agcgtgccgc tcagcaatcc cacgaccaga
      781 agcgccaacg catacacacc gaacgtctgg aacacttctg ccgcgctgac gtgccccacc
      841 gccacactgc cccagcttga cgcaaagcca tagagctggg catttgtcac cacaccgaag
      901 acaatgatgc aggtgcatgc cgcaaacagg ccgacgcggc gctgtgggat gaggctggcg
      961 acggcaaccg tgatcacata gaggaccgcg aagggactct cgactcctcc agtgcgcgcg
     1021 accagcactg tttcgaggag gaaatcgatg ccgacttgga tccagaagaa cgttgtcagg
     1081 gcggaagggg tctttagcgt acgaaggaag accgcggagg gaatggtcag tgcataggta
     1141 aggacgatga gtgtataaaa ggtgtcaaca cgttcgcctt tcgtcgcttg aaagatgagc
     1201 gacaagccca gcatcagggt gacaaaggtc acccgaagcc ccatcaacca ctgaagcctg
     1261 gctcgcagag catccatgcg ttgttacagt tcgaagaggc ccacccggac cgggcgtgtg
     1321 gggaaaaccg gcgaagatag gcgcaagagc ctacccaatc gcggaggcca tcttgaagat
     1381 cggaagatac atcgcgatga caatgaatcc gatcacgacg cccaggaaca ccatcatcat
     1441 gggttcgagt aatgacgtca gtgaggccac cgcctgatcg acctcatctt cgtagaaatc
     1501 ggcgatcttt ccgagcatgg catcgagtgc gcccgtggat tctcccactg caatcatgtg
     1561 ggtgaccatc ttggggaaca cctgactctg cgtcaacgga tccgcaatcg tttttcctcc
     1621 gctgatgctg acgcgtgcat tcaacagcgc ttcctcgatg accttgttgc cggaggtttt
     1681 ggcacaaatg gacaatccgt cgagaagagg aacgccgctg gaaagcaacg ttcccagcgt
     1741 acgggtgaac ttggcaacgg acgctttccg aatcaggtcg ccaaatattg gcgtcttcag
     1801 cagaaacttg tcgattgcca tcctaccctt tggcgtagcg tagtatttct tcacgagcat
     1861 caccgctccg atgatgagcc cgacggcaat gtaccagaac gattgaaagg cattgctgat
     1921 gtcgatgacg agctgcgttg gccccggcaa accgactttg ccgccggaga gctcttcgaa
     1981 catcttcgcg aaaatgggaa tcacgaagat catcaggacg gtgatcacga tgcccgcgac
     2041 gccgagaatt gccgacggat agatcatggc cgacttgatc tgccccttga gcttcatggc
     2101 cttttcaata tgctttgcga ggcgagttaa aatggtgtcg agcaagccgc ccacctcacc
     2161 tgcatgcacc atgttcacgt acagatcgtc gaatactttg ggatgccgcc gcaatgcgtc
     2221 ggaaaaggtg gacccggctt ccacttggcc tctcacttca cccaccgttt cgcgcagcac
     2281 tttattttca gattgcgtgg aaaggatttc gagacattgc acaagcggaa gaccggcatt
     2341 gatcatggtt ccgaactgcc gtgtgaaaac caccaggtct ttgtcggtca ggccggaccc
     2401 gaagctgagc ttgagccctc ccttcccggt tttttcttcc aaactcgtga cgacaacgct
     2461 ttgcttgcgg agctgatcga ccgcctcatc tttcgtcttg gcagtcagtt cacctttctt
     2521 gaccgctccg gaccgtgaac gcccgacata tgcaaaggtc gccataagtc actctcacct
     2581 tttttgagag tccgctcaac aaccgaattt tcgatggaaa tacctacgtg gcgagtctac
     2641 tggtgacctg aaaaactgtc aaacaaatgc acaatttgtg cggagtagcg gtgcagagag
     2701 caccggctaa ctagctatca ggttggagag gcaccaaccg atcggtaccc acgataaaga
     2761 taatgacagc cggaggagac cgtaaatgcg accatggcga ttacgagcgg attcagcact
     2821 gacagatcca agtggcgcga gctcaaaaaa atcacaagca gcacgtagga gagttgcaag
     2881 aaggtggccc cttttccgaa aaatgtcggg gctatatcaa tattcgactc tgtgaactgc
     2941 gccacggcgg ttcccagcag aagcatcgca tcgcgactga ccacaagaat ggtgacccag
     3001 gaaggaatga gatggatcgc ggaaagcgtc acgaaccctg acgtcagcag cagtttatcg
     3061 gccaacggat ccaggaacgc gcccaagcga gtccgttgat tcgccgcgcg agcgatggtt
     3121 ccatcgagcg catccgtcac gccggctacc agcaggacga tcatggcccc atcgaaccgt
     3181 tcgtaaatca ataacccgac gtagaccgga atcagcaaaa tacgaagaat ggtcagactg
     3241 ttggggatat tcatgcggtg taggaagcat ccggcgccgc gctgagcaac gaaataatag
     3301 gaggggtgag caatgaagtc aacggcttgc agtcggcggt cgatcgccct atactgcaac
     3361 tccgcctaca cgaggaaatg acgatgaatg tgctggcctc gctgtttaaa agagacggac
     3421 tagtcgaaaa acatcagttg gaaggggttg atcccagcga tcgttatttc aaccgagccg
     3481 ttctcgtcaa ccgcaccgct tccggctact cggccaagat catgtacgag gcactcaccg
     3541 tcgaaggcca atcacatccc acgattgccg ccgccattac tgagcttgtt gaaaaattgc
     3601 agtcgcttgg cttcagccga ctcaggacca gggccaactt caaaggcacc aaatatctcg
     3661 cggaaaaaga aacctggatc gagtatcagg acctcgcttg attcacctcg tcccgctcac
     3721 gatttcctga aacaccatat catgaatcag cgggcggaag gcccggatcc cttcattctt
     3781 ccgggttgga tggacgcgat tggtgagtaa cagtacttcc aattttctcc ggggatcaat
     3841 ccataacgag gtccccgtat atcccagatg gccaaaggat tccggagaca agtggctgcc
     3901 ggatgacgaa ggcgccgtcg gcgtatccca tcccaaagcc caactgaagt gcgaacaagg
     3961 tgacgacctt cgcgtgaact cgcgaacgag tctctgctca agcatcgacc gctgtccttc
     4021 ataggagcga agccagacac cagagacagc cagcacggct tccgcagtgc cgaacaaccc
     4081 tgcgtgccct gcgacgccgc ccatggcagc ggcattctcg tcatgcactt gtcctttcag
     4141 caattgccgc ctccactcat cccattccgt cggggcgatg ctcgagacat ccaccgccgg
     4201 tcgagccgga tggcaatccg tcgcagtagg attgaagcac agtggttgcg ctccaagcgg
     4261 cttggccacg gactcgtaaa aatactcgtc cagcggctgc ccactcgcgc gttcgaccgt
     4321 taagcccagc agaataaatc caaggtcgct gtactcactc ttctcgcctc tccggtacat
     4381 caatggttct tcgccgatga ggtgaagcac tgtttgtcgc actgattcag atcggcccca
     4441 tgcgatgcct ttggctgtga gtccctcata caacggacgc catccgggaa gaccagagcg
     4501 atgttgaaga agatctcgta gggtagctgc cccgatggga gcgccctcaa gatcgccgag
     4561 agtgtgtttt acgggtgcat cgaggtccag ctttcctgat tgcacaagga gaagaatcga
     4621 ggtcacggtg gccaacggct tagtcagcga tgccaggtcg tagactgtgg aaatctgtac
     4681 gggatcgtcc ggatccgacg agagacagcc ggctacgaca aggctcttaa gctcaccggc
     4741 caaccgcacc gccaacaccc cccctgggaa tacgccgtcc cgcaccgcct gatccattgc
     4801 ggcctgaatg acacttggtc ttttcatcga caatggccgc aggcgactga agaagcgact
     4861 tgaaagagcc gtggatccct ccccgtggac cggaagccca acaatgcgcg aggcgttcgc
     4921 aaggggagag gttcggaaac acgcgaagaa gaaggggtgc gaaggaagga tcgctttaac
     4981 atcctctcac aatgcagggc tcgattctcc ctcgagctta tgctcggcac tcgaggcacc
     5041 gtcttggaat gcctcctgtt cctccacttc caacatccgc tcgaacgtgc gcaacgtcgc
     5101 acgcaaccgt tcgaccatta agagccgctg cttttgcagc gcagagagat cacgctgggt
     5161 gttagacatc tcgacccgag cctgacgtat catctcgccg gccttgagct cagcttcttt
     5221 gatgacgagc tccgactccc gttgcgcgct gcgctttaca tcctctgcga gcgactgcgc
     5281 agacacgagt gtgttggaca gggtaacctc ggtccgcttt aattcgatca actgctgctc
     5341 gagaaacgtg attttttctc tctgcactgc attctcacgg ttcagctctt cgaccgtctc
     5401 ggcaatttct tccagaaatc gattgacttc ttccttgtcg tagccgcgga aaccggtctt
     5461 gaagaccatt tgttgaatgt ccagcggtgt gattttcatg gctgccctcc tctaggatgc
     5521 ttatccaatc acagttgcca tttgatgaag agaaggcaca ataaatctct gtaaaaatat
     5581 gagaataagg ataaggataa tcggagacac atctacgcca agcctccaac cgatcatcct
     5641 tcggatggga gccaatgccg gttcagtgac tttttcaaga aactgcacga tcggattcca
     5701 cggatcaggg ttgacccagg aaatcaccgc acgggcgatg acgacataca tatacaactc
     5761 caagagataa tagacgatcg aggccaatgc cgtgaagaaa ttttcgacga caaacatcag
     5821 cgcgcaagct cctgcgaacg ttttgtcgcg gcttcgacag cccccatcag tgtagcacgg
     5881 aacttgccct gttcaagctg atgcagtccg gcgatcgtcg tgccgccggg cgaggcgact
     5941 tggtccttca gtgttgccgg atgggtgccg gtctcaagaa ccattctcgc tgcgcccaga
     6001 acagtttgag cggccaataa ctcagccgtc tgacgaggca atcccatctt gactccgccg
     6061 tctgccaagg cttcaagggc taggaagaca tatgcagggc cgcttccact gagcccggtc
     6121 acggcatcca taagacgctc ctcaacggga atcacgcgcc cgacggcttc gaatatcgtg
     6181 cgggcgacga gactgtcctc ttccgttact tctctacccc atgacaacgc cgtgatgccc
     6241 tcacggacga gggcgggcgt attcggcatc gcacggatta tgcgtgtacc gggcgacacc
     6301 tggctagtaa ttgactgaat cgtcacaccg gcggcaatgg aaatgaccag caccccattg
     6361 agatcggcgc cgatctcgct caacactgtc ggcagcgatt gaggcttgac agccaggatc
     6421 acaatgtttg cccatgtcac ggcctgccgg ttctccgctc cgacacgaat tgaaaacaga
     6481 ctcttcaacc gatcacggcg agtggccacc gggtcggacg cccagagtgc atccgagcca
     6541 cagacgcctg cggcgagcag gccgccgatc atggcctcgg ccatgtggcc actgccaata
     6601 aaggcaatct ttttcaccat caaactagtc acgtcgctct ccgaacagtg ctgtgccaag
     6661 gcgaaccaaa gttgcccctt cttctatcgc aacctcgtaa tcatgcgaca tgcccattga
     6721 gagttcatcc atcgagatcc gagaaatctt cagttgtatt aggcgtgccg ccaagtcccg
     6781 gagtgctcga aaatagcgcc tggctcgctc tggctcgcgc atcaatggtg gaatcgccat
     6841 caatccgcac accgccacat gatcaagtcc ccccagctgg ggaaggactc tctccacttg
     6901 atcacctaga aatccggctt tgctgctttc ttcgccgaca ttcacttcca gcaggatctt
     6961 ttgccgtaat ccggccgctt tcgcccgccg atcgatctca cgagccagct ccaggctgtc
     7021 gacggaatga atcagatcaa aaatcccgac gacagagcgg acctttcgtc gctggagccg
     7081 gccgataaag tgccagatga cgcccgtgtc tttcaacgcc tcgatttttg gaagggcctc
     7141 ttggagccgg ttctctccgg caagtgtcag tcccgctctc acagcccttc ggatgtcacc
     7201 aaccgggact gatttcgtgg cgccgaccaa ttgcacactg ccgtccgccc gaccggcctt
     7261 gcgcgtcgct atacagattt ggttgaaggc gactgcgacg cgattctcga ttgagtcacc
     7321 ggcttcggca tccatctttg cccgatcaag cccactgctc cttggaatgc gtcgccgtat
     7381 tgtacaccgg cctaccggtg aggcatcaac gtgattccgt tgagcatggt gccattcacg
     7441 cgcccttcac gccggtagga gaaaaataga tgaggatggc agatcgtgca gagattgacc
     7501 gtcgtaatgt gttcacggtc aatgccgatt tgggtggcct gtctcttcac caaggctttc
     7561 aagtcgagct tggcgcggtt accgcgacga tccttgatga gcgaagagca ctcggttggg
     7621 cccctgcgta acggctcgag caccgcttca tcgacctcgt agcaacagga tcctgccgac
     7681 gggcctatac tgacgcgcac atcggcctca tcggtcgaga atcgggagat catcaactgg
     7741 atggtcttag gtacgatgcc ggccaacgcc cctcgccatc cggcatggat ggctgctgcc
     7801 actttccggc gccggtcgta gacgagtacg ggaacacaat ccgctgtacg aactgcgacc
     7861 gtcacactag gctgatccgt gatcaacgcg tcccatccac cttggaatcg gtcggatccg
     7921 gtcagcggac gatcaacgat cagtgcatcc gtgccatgca cctgtttcac ggagagcatc
     7981 cagctctgcc cgctgacatc acgagttcgt gcaggcacgc cggtttcata cacgaccgcc
     8041 tcagtatggt gccgcgtgcc gaaaaaatgc ctcacgccgt catgcggcaa gccaaaggca
     8101 ggcacggtga tcaccgaggc ggtcacgctg ctgctccctc atccgaccaa cggcgaatct
     8161 tcgcttagtc ggcctgcttt ctcaaaaacg tcggcacatc ccactcgtca tccgccacca
     8221 cggccagtcg ctccatggat tcacgatgat ctcccatccg gcgcagatag gtcggacgat
     8281 ccaaatcctt atgcggccgg tcggcgaccg ccacgccatt gaaaggcggt tgcgcagctc
     8341 ttgccatgcg agcttggctt gcaactccca ccggcatcgg ctgatcgtct cgctcaaagc
     8401 cggtcgcaat cacggtgacg accaggtcgt cgcccatgtc gggattgatg acctgtccga
     8461 caatgatgtt cgcctcagga tccgcggtct gttggatgat ggaagccgcc tcttcaattt
     8521 catgcagcga catattcggc ccaccgctga tattgagaag cacgccgcga gcaccttcca
     8581 cgctgccttc ctccagcagc gggctgcaaa ttgccttttg cgccgcttcg atcgctcgat
     8641 tcgtgcctcg agaaacaccc atgcccatga cggcccggcc ggtatgagac atgacggtac
     8701 gcacgtctgc aaagtccacg ttgacatgac cggtcgtcgt aatcacgtct gcgatgcctt
     8761 gaatcgcctg gcgcagtaca tcgtcggcga ctttgaaggc gtccaacaaa ggcgtcgcct
     8821 tgtcaacgat gcccagcaaa cgttggttgg gaatgacaag caatgtatcg acgtgacgtc
     8881 ggagatcgcg aatgccttca tcggcgtgag tcattcgtcg atggccctca tacgaaaacg
     8941 gcttggtgac gacgcccacg gtcagaatcc ccagttcgcg tgcgatgctc gcgacgatgg
     9001 gtgcggcccc agttcccgtt cctcctccca ttcctgccgt cacgaaaacc atgtcggcgc
     9061 cctccaggca ttctcgaatc tgatctttgc tctcaagcgc cgattctttt ccgatttctg
     9121 gcttcgctcc ggctccgagt cctcttgtcc gctcaggtcc aagttgaacc ttatacgcgg
     9181 cacgggaccg atccagcgcc tgcaggtccg tattcgcggc aatgaaatcg acgcgggcca
     9241 agcccgacgt gatcatcgta ttgaccgcat tgcagcccgc gccccccact ccaatgactt
     9301 taatgcggac gggcgacaga caatcttctt ggaatgagaa catcaggaca cctccattgg
     9361 cgcgagggag tccgcgcgcc ggttaagtta aaagaactcg aacatccagg acttcattcg
     9421 atctaacatt ttgccgattg gccgcccgct cctgagtccc accgtggcgg tttcttcggt
     9481 atgtcgtcgc gcgtgcaata gtaatcccac ccccgtcgca tgcatgggat tgctgacgat
     9541 gtcccgcagt ccaccaatgc cggtaggcac tcctcgacgg gcgggaaggt tcagcacgtg
     9601 ctcagcggca tccggcatgc cttccagtaa ggaagtgccg cccgtcacga cgactccggc
     9661 gcccaacatc ccttcatagc cggcacgggt gatctctcgc ctgaccaact cgaacatctc
     9721 ctcgacccgc ggctcgagaa tctcggcaat atcgcgccgc gagaatgttc gtgctgggcg
     9781 gtctccgact gaaggcactt ccaccgtttc atgaccctgc accaattccg tcctcgtcac
     9841 gccgtgctgc actttgatct tctcggcttc ggtctgagag gtgagcaacc cgatggccag
     9901 atccttggtc aggttttgtc ccccgatcgg cagtacggcc gaatgtcgga tgcttccatc
     9961 cagaaaaatg gccaggtcgg tcgtgccgcc tcccagatcc accatggcca cgccaaggtc
    10021 tcgctcttct tggctgagca ccgcttcgct cgaggcgaga ggctgcagga tgatgtccac
    10081 gacgtcgagc ccggcgcggt tcacgctctt gatgatgttc tgtgccgacg tgacggcacc
    10141 ggtgatcaca tggacgttca cctccaggcg attgcccgat agtcccaacg gctcgcggac
    10201 gccctcctgc ccatccacca tgaattcacg gggcaacacg tggagaatcc ggcgctcgtg
    10261 aggaatgacg gccaacgttc tggcgctttc gatcgcccga tggatgtcgt cgcgcgtcac
    10321 ctcagacttc ttgagcgcca cgacgccttt gcagttttca gcagagatgt ggctgccggc
    10381 gataccggtg tagacggaat tgatctgtac cgccgccatc aattccgctt cttcgaccgc
    10441 cttcttgatg gactccacgg tgctctcgat atcgacgacc acgcctttgc gaagtccgcg
    10501 cgacggactc gaacccacgc caatgatgtt caaagtccct tcctcggtca cttcggaaac
    10561 gatcgcgcag atcttcgtcg taccgatatc caatcccacg agaatttgat cccgcttcgc
    10621 cacagcctca ccccctttcc ctgacgatca cccggttgtc atagcgcaga tctacgtcgt
    10681 gaccctcatg tttccgtcca tcgagagccg gcatcctgaa cgccgctttg actttcatga
    10741 agcgttccca ctgatccttg agcgcgtcgc taccgaaatg aaatcgtact cctctggcgg
    10801 aggcgacaag atttacgggg tgagccacgt cgagctcgat acgtccatct acggattcgg
    10861 ccatccatct tgccagctcg actgcggact gaatccgctg cttgattcga agatcacctt
    10921 gcgccaagag cgtgccgtcc acgcccgtca agagtggcag ggtcgcctcg tcgcgggtgc
    10981 caaggcgggc gaggatgacg ccttcgctat cgatgaggac ctggtcggac tctagacgga
    11041 caatggctgc gggcgtccgc tcaacgaccg tcacgcgcag ctcatgcaat ggcaaccgct
    11101 cgacggtcgc ttccttgatc cagggatgaa tccggacacg ctcggcgaga taagacaggc
    11161 tgacctgata ttgagcgagc cctttcttca aggccaaccg gtcgatcacc tcctgcttgg
    11221 tgacttggtg cgtcccttcc actgatacct gtttgatctc gagccctcgg tgaagcatcg
    11281 gtccgagctg ttgcgagagc atgacgatca tccagcacac ggcaactgct ccccccagcc
    11341 atccggagag gcggagggct cttcggcacc gtgtcagccg ctcgatccgt ctggcccgct
    11401 gccggtgatc catcgactga gacgctcgtg ggcctttcca ttcattactg cgatggccgg
    11461 ctgcttttgc gggcggcttg cgcctaagaa gtctcatgcc gcgtgctctt tatttttcat
    11521 cctggggagc gcggattcga gaatccgctc cgtcaattct tcgtagccaa tgccggcctg
    11581 aaccgcggcc attggcaaaa ggctggtttc cgtcatgcct ggtacggtat tgagttccag
    11641 cacatacggg cgtccgcggg gcgtaatccg aaaatccacg cgggcagccc ctgcacagcc
    11701 caagacttcg aatgcccgca acgccaagac gccgatttcc ttcgtgacgg cagccggaag
    11761 aggtgccggg cacaggtatc gggtcttccc tttttcatac tttgctgaga aatcgtaaaa
    11821 cccgcccgga gcgacgatct ccac
//
LOCUS       DASTQY010000282         7343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_777980, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000282 DASTQY010000000
VERSION     DASTQY010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777980"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFR82_09420"
     CDS             complement(<1..242)
                     /locus_tag="VFR82_09420"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEU4501858.1"
                     /translation="MPVIDVITEYAVRYGFQALGAAIVFAAGALVARWGGKLTRQWLQ
                     RQEMEPPVRMLLVKTVQVVILLFAVMAALGQLGVQIA"
     gene            complement(480..1127)
                     /locus_tag="VFR82_09425"
     CDS             complement(480..1127)
                     /locus_tag="VFR82_09425"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4501859.1"
                     /translation="MKRPTSQLLEWSMILGLMLWLAPVTPAVAALPQTPTESVRSTIE
                     DVIRVLTNEQLKQPDRLAERRQEIERIVRDRVSYEDMAKQSLGLPWLNLEAEERQEFV
                     DLFVQLLRDSFAGKIDTYADEQVLYLSEQRDDRMAEVRTKLAGRKTHSLLNFRLTDRA
                     GTWRVYDVVIDGASLVHNYRAQFARIIRDVSYPGLVKLMREKTLVVKAFEPGHTD"
     gene            1576..3645
                     /locus_tag="VFR82_09430"
     CDS             1576..3645
                     /locus_tag="VFR82_09430"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEU4501860.1"
                     /translation="MPALSYHHPAVKVTAWVLIACAYFLAGKIGLGFASVNVSASPVW
                     APAGIAVALFLLLGFSIWPAILAGAFLVNITTAGTVLTSANIAVGNTLEGMLGAWLLQ
                     RFAHGIRAFEAPPDILRAALLAGVLAPTVSATIGVTSLTLAHYASPHDYADIWLTWWL
                     GDATGILIVAPAILLWARHPRIEWTSRERLELGLLLLVLVVTCTVMFGPFIYTGDRYS
                     LRVLLVPILIWAAFRFGPRETATLAVLLAGWVTWGTINGRGLFTGDSENEAFLFGQAF
                     LGVNTVMALALAAAVQERKEAEVRFQATAERLVQKRTEELLHTQARLRTMAQALTLTE
                     QRERKRMAGELHDYLAQLLVLSKMKLSRVHAQIQTIGGPVAAVVEEIDHTLAKALDYT
                     RTLIAELSPPVLHELGLPMALQWLADQMKKHQLTVDVQLGTSQLPLTENQAILLFHSV
                     RELLINAAKHAGTNKATLTLDAADNMLTIAVQDQGKGFKTDRIEPQQPGQHFGLFSVK
                     ERMEELGGWLRVESAPGVGTTVTLGLPLTPAASSPAPSGNERQVPPPAKATGVRRVLL
                     VDDHAMVRQGLRAVLDAYPDLFIIGEAADGREAVSIAKKRTPDVIIMDINMPRMDGIE
                     ATRRIKKEQPGTVIIAVSVNDTPDLRESLQKAGASAFVSKDEAGERLYETIITMALPQ
                     QEARGLT"
     gene            3659..4273
                     /locus_tag="VFR82_09435"
     CDS             3659..4273
                     /locus_tag="VFR82_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501861.1"
                     /translation="MRSLFVLHSALLLLMLVSPAPSYDQSFRLGPEDEWPASQQRHAP
                     FHTVREKNDARPDPSNEMLRDGEYGPAREIKRFTGPTIQLRGTAEEIGLDGRTLASAL
                     EVKFLQDFAFLQANFAFDKTYETWEIGLFDCEAWTVGHTYPIAFHLQCVAGSMDEPRH
                     WRYATLGYGPKEKIAETIRKALDAIIVEYASFVRKAHGKKDGGT"
     gene            4818..5342
                     /locus_tag="VFR82_09440"
     CDS             4818..5342
                     /locus_tag="VFR82_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEU4501862.1"
                     /translation="MTQHKTRWIACLSLMLVCACSPNIAVDYDKSTDFSRYRTYGWGK
                     GTPSKNPELDRQIVEAIDDQIGHKGLQKTNGDPDLVVTYHAATHEEIDYNEASYSSGS
                     GPAYGAPINSSSADAPMRVRVGTIVVDMYDTTKKRNVWHGVGTDLVGEDPAKTSEEIR
                     KGATKMFENFPPRQ"
     gene            complement(5399..7006)
                     /locus_tag="VFR82_09445"
     CDS             complement(5399..7006)
                     /locus_tag="VFR82_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501863.1"
                     /translation="MLQTDHAHATQPHRPRWFVRQDVDGLLGLALDNLIQILLIVGLC
                     RGLLGYSSELVYGRILPAVALSLIVGNVYYSWLAYRLGVKEGRDDRTALPYGINTVSL
                     FAHVFLVMLPVKLIALDRGATMDEAIALSWQAGLVACLGSGVIELIGSVLADPLRRWL
                     PRAALLSTLAGIGLTFIALGFLFRTYEYPVVALVPLGIILLTYFGRVQFPIPGGLLAV
                     LVGTALAWMTGLVRPDAAMFSQALEPLGWHPPGLFIEALWQSREILFTQLSVILPMGL
                     FNLVGSLQNLESAEAAGDAYPTAPCLAANGVGSVVAAVCGSCFPTTIYIGHPGWKAMG
                     ARIGYSWLNGVVMALLCVTGAAAVVAAIIPIEAGMAIVLWIGIIIGAQSFQATPSKHA
                     PAVIMGLLPGIAGWGAQLIKTSLRAAGLATVDRPFSPDLLPIFARADIYLGGAFALEQ
                     GQILSAMLLAAMTVFIIERQFAWAGACMLVATAFSWVGLMHSYRWMPSDTVLQPGWGS
                     GSPWATGYLLLALLFYWAAWKERSKAC"
BASE COUNT         1685 a         2124 c         2141 g         1393 t
ORIGIN      
        1 gcgatctgca cgcccaactg gcccagcgcg gccatgacgg cgaacagtaa aatcaccacc
       61 tgcacggttt tgaccagcag catccgaacc ggcggctcca tctcctggcg ttgaagccac
      121 tgtcgcgtca gcttgccgcc ccagcgagca accagcgctc cagccgcaaa gacaatcgcc
      181 gcccccaacg cctgaaaccc atagcgaacg gcatattcag tgatcacgtc tatgacgggc
      241 atcgcacacc tcctcaatta aacagaattt ttaaatgcca agtatgacgg cgggttgaat
      301 cagccgctca tccacggtcg cgacagacct taaggaatag ccggagcata catctggacg
      361 cgcaaacttg aaaagcagcg aggagcactc aggaagggtg tattccaaac acgaagagag
      421 caagcggttc agcggcggtc gtaggtgaac ctctcgagag cgtaaccttg tttattttcc
      481 taatcagtgt gccccggttc gaaggctttg acgacgagcg ttttctctcg catgagtttc
      541 accaggccgg ggtaggacac atcgcggatg atgcgcgcga actgcgcgcg gtagttgtgc
      601 acaaggctcg cgccgtcgat caccacgtca tacacacgcc acgtgccggc gcgatcggtc
      661 agccggaagt ttaacaagct gtgggtcttg cggccggcga gcttcgtccg gacctcggcc
      721 atgcggtcgt ctcgctgttc ggagagatag agcacctgct catcggcata ggtatcgatc
      781 ttcccggcaa acgaatcgcg cagcaattgc acgaacaaat cgacgaactc ctgccgctct
      841 tctgcctcga gattcagcca tggcaggccc aacgattgtt tcgccatgtc ttcataactg
      901 acgcggtctc gcacgatccg ttcgatctcc tgacgccgct cggccaaacg gtcaggctgt
      961 ttcaactgct cgttcgtgag aacacgaatc acgtcctcaa tggtacttcg caccgattcc
     1021 gtcggcgttt ggggtaacgc ggcgacagcc ggcgtcaccg gcgcaagcca cagcatcagg
     1081 cccaggatca tgctccattc caagagctga gatgtcggtc gtttcatcga attcatgagc
     1141 cccttaggtt aagacgtcat ccggttcgcc ggttgcaaga gcgcaagatc agtgccgaaa
     1201 tggcgatcta tcgtgagcta taaagaatct aaaaagcgcc acaaatccgc gatgctatcg
     1261 gctcggaaac aattctgggc ctaaaggctc agaaggaaaa aagcttcagg tgtggtagca
     1321 aacactcaga ttaaggcctc gaagaaaggc gcgatcacca gtggccgttt gcgaatgcca
     1381 cccgcttttt tctctcccaa cgttccaatg ctcacccaac catttgtgag tgtgcaggcc
     1441 aatgttcatg tgaagtagtc gaagcggtga agaggaacac cttcgctgag gacgattgag
     1501 ccgcgccacg tcaatgccaa tttgaactag gggtgccctt cctttatagt gagtgtttcg
     1561 tcaccccaca tcaacatgcc agctctgtcc tatcatcacc cggccgtgaa agtgacggcc
     1621 tgggtgctca tcgcctgcgc gtatttcttg gccggaaaga tcgggctcgg cttcgcctcg
     1681 gtgaatgtca gtgcatcgcc ggtatgggcc ccggccggca tcgctgtcgc cctcttcctg
     1741 ctcttggggt tctccatctg gccggcaatc ttagccgggg cgtttctggt caatatcacg
     1801 acagccggaa ccgtcctcac gtccgcgaac atcgccgtcg gaaataccct ggaagggatg
     1861 cttggcgcgt ggctcctgca acggtttgcc catggcattc gcgcgtttga agctccgccg
     1921 gatattctaa gggcggcgct gctcgctgga gtgctcgcgc cgaccgtgag cgccaccatt
     1981 ggcgtcacca gcctgacgtt ggcgcactac gcgtccccac atgactatgc ggacatctgg
     2041 ctcacttggt ggctcggcga tgcaacgggg attctgattg tcgcaccggc aatattattg
     2101 tgggcccgcc acccgcgcat tgagtggacc agtcgagaac gactcgagct tggcctctta
     2161 ttgctcgtgc ttgtggtgac ctgtacggtg atgtttggtc ctttcatcta tacgggagac
     2221 aggtattcgc ttcgggtcct gctcgtgccc atcctgatat gggcggcgtt ccgattcggt
     2281 ccccgagaga ccgctacact ggcagtgctc ctggctggat gggtcacctg gggtaccatc
     2341 aatggccgag gcctcttcac cggcgactca gaaaatgagg ccttcttatt cgggcaagcc
     2401 tttctcgggg tgaatacggt catggccttg gcactcgccg cggccgtgca ggaacgcaag
     2461 gaggctgagg ttcgattcca ggccaccgct gaacggttgg tacagaagcg caccgaagag
     2521 ctgctgcaca cccaggcccg attgcgcacc atggctcagg ctctcacctt gaccgaacag
     2581 cgagagcgca aacgcatggc cggcgaactg cacgattatc tcgcccaact gctcgtgctc
     2641 agcaaaatga agttgtcgcg agttcatgca caaatccaga ccattggcgg tccggtggct
     2701 gccgtggtcg aagagatcga ccacacactg gccaaagcac tcgactacac gcgcacgctg
     2761 atcgcggagt tgagcccgcc ggtcttacac gaattgggtc tgccgatggc gctccaatgg
     2821 ctggcggatc aaatgaagaa acaccagctg accgtggacg ttcaactggg aacatcccag
     2881 ctgccattaa cggaaaatca ggcgatcttg ctgttccatt ccgtccgcga gctgcttatc
     2941 aatgccgcga aacatgccgg gacaaacaag gcaaccttga cgctcgatgc ggcggacaac
     3001 atgttgacga ttgcggtcca ggaccaggga aaaggattca agaccgacag gatcgagccg
     3061 caacagcccg gccagcactt tggcctcttc agcgtgaaag aacgcatgga ggaactggga
     3121 ggatggctgc gagtcgagag cgcaccaggt gtgggaacga ccgtcacgtt gggactgcca
     3181 ttgacaccgg cggcgtcatc tcccgcccct tcggggaatg aacgacaggt gcctccaccg
     3241 gcaaaggcga ccggcgtgcg gcgggtgtta ctggtggacg atcatgccat ggtccgccaa
     3301 ggacttcgag ccgtgctgga cgcctatcct gatctcttta tcatcggcga ggcggcagat
     3361 ggccgggaag ccgtgtccat tgccaagaag cggacaccgg acgttatcat catggacatc
     3421 aatatgccac gcatggacgg gatcgaagcc acaaggcgga tcaagaagga acaacccggg
     3481 acggtcatca ttgccgtctc ggtcaatgat acgcctgacc tccgtgaatc gttgcaaaaa
     3541 gccggcgcct ccgcgtttgt atcgaaagat gaagccggtg aacgactgta cgagacgatc
     3601 ataacgatgg cacttccgca gcaagaggcc cggggattaa cctgatacgg ataattggat
     3661 gagatcattg ttcgtccttc atagtgcgct cctgctcctg atgttggttt ctccagctcc
     3721 aagctatgat cagtcgtttc ggctgggccc tgaagatgaa tggccggcat cgcaacagcg
     3781 acatgctcca tttcacacgg ttcgcgaaaa gaacgacgca cgacctgatc cctcaaatga
     3841 aatgctgcga gatggtgagt atggaccagc acgcgagatc aaaagattca ccgggccaac
     3901 aattcaattg agagggacgg cagaagagat tggactcgac ggccgcacac tcgcaagtgc
     3961 gcttgaagtg aagttcctgc aagattttgc ctttctccaa gccaattttg ccttcgacaa
     4021 gacctacgag acgtgggaga tcgggctatt tgattgtgaa gcgtggacag tgggccatac
     4081 gtacccgatc gctttccatc ttcaatgcgt agctgggtcg atggatgaac cccggcactg
     4141 gcgctatgcc acactgggct atgggccaaa ggagaaaatc gccgagacaa tcaggaaagc
     4201 gttagatgct attattgtcg aatacgcctc ctttgtgcgg aaagcgcatg gcaaaaaaga
     4261 tgggggcacg tgagggcggt tggtctcctg tgctcgccga aggcgcagac cggggatggc
     4321 ggttattcgt ctcccttgct cgcccaaggc gcacaccaga agaagggatc agcctggtca
     4381 gtccttgtgc tcgcggaacg cggcacacga gattgatcgg atttttagat aggcggtgct
     4441 cgttcacgcg cgcacgtttg gactgaacca ggctgctcct gagaggacga acaagctagc
     4501 tcggatggat cactggtaac gtggtgctcc ttgaagcgcg cagtcggaga cgaattaacc
     4561 accatcttca agaaacaaca agctagcctg ggaggaacat taagcgcgca attcgagacc
     4621 aatcagccct catcccccga gaacgcagcg agcgagcttg gaaggagcat tttaatggcg
     4681 ctcctcgcct gtgaacggct cgtcgccggg aaatcgtgag ggcaatcggt ggttggctgc
     4741 gtggaagagc aaggattgcg agcttaggca agacagagta gcatgcacta cctgtattga
     4801 agacaagagg tagtcaggtg acacaacaca agacacgatg gatcgcatgc ctcagtctca
     4861 tgctggtgtg cgcctgctcc ccgaacattg ccgtcgacta tgacaaaagc acagacttct
     4921 cccgctaccg cacctacgga tggggcaagg gcacaccatc gaaaaacccg gagctggatc
     4981 gccagattgt ggaggccatt gacgaccaga tcggtcacaa gggcttgcag aaaacgaacg
     5041 gcgatcccga tctagtcgtc acctatcacg ccgcgacgca cgaagaaatt gactacaacg
     5101 aagcgtcgta cagttccggc tctgggccag cctatggggc gcccatcaac tcgtcgagtg
     5161 ccgacgcgcc tatgagagtt cgagtgggca caattgtcgt cgatatgtat gatacgacaa
     5221 aaaaacgcaa tgtgtggcat ggcgtcggaa ccgatcttgt tggggaagac cccgcgaaga
     5281 cttccgagga gatccggaaa ggtgcaacaa agatgttcga gaatttccca cctcgacaat
     5341 aatctccaag ccctcgcttc cccgtcttct cgattcgtcc agtgcctatc tcggcagatc
     5401 aacaggcctt actccgctct ttccaagccg cccaatagaa gagcagcgcc aagaggagat
     5461 atccggtggc ccaggggctc ccgcttcccc agcccggttg caggaccgta tccgacggca
     5521 tccagcgata gctgtgcatg agacctaccc acgagaaggc cgttgccacg agcatgcagg
     5581 cacccgccca cgcaaattgc cgctcgatga tgaacaccgt catggcggcg agcagcatgg
     5641 cggagagaat ctggccctgc tcgagtgcga aggcgccgcc aagatagatg tccgctcgcg
     5701 cgaagatcgg caggaggtcc ggactgaacg gccgatcgac ggtggccagc cccgccgccc
     5761 ggagactggt cttgatcaac tgggcgcccc accctgcaat gccaggcaag agtcccatga
     5821 tcacggccgg cgcatgtttc gagggcgtgg cctggaaact ttgtgccccg atgatgatgc
     5881 cgatccacag gacgatggcc atgccggctt cgattggaat gatcgcggcg acgaccgcag
     5941 ccgcgccggt cacgcagagc agtgccatca ccacgccgtt gagccacgaa tagccgatgc
     6001 gagcgcccat cgccttccat cccggatggc cgatatagat ggtggtcgga aaacaggagc
     6061 cgcagaccgc cgccacgacg gacccgactc cattagccgc aagacaggga gccgtcgggt
     6121 acgcatctcc agcggcttcg gcgctttcca aattctgaag actccccacg agattgaata
     6181 gacccatcgg caggatgacg ctgagttgcg tgaacaggat ttcgcgcgat tgccatagcg
     6241 cttcaatgaa taagccaggt gggtgccatc cgagcggctc gagggcctgc gagaacatgg
     6301 ctgcatccgg acggaccaat cccgtcatcc aggcgagcgc ggtcccgacc aggaccgcaa
     6361 gcaggcctcc cgggatcgga aattggaccc ggccgaaata cgtgagcagt atgatgccga
     6421 gcggcacgag cgctacgacc ggatattcat aggtgcgaaa gaggaagcca agcgcgatga
     6481 aggtcaatcc gatgccggcg agcgtcgaaa ggagcgccgc gcgcggcagc caccggcgaa
     6541 gcggatcggc gagcacgctg ccgatcagtt cgatcacgcc cgatcccaga caggcgacga
     6601 gcccagcctg ccaggacaag gcgatcgcct catccatggt ggcgccccgg tcgagcgcga
     6661 tcaatttcac cggcagcatc acaagaaaga catgcgcgaa gaggctgacc gtgttgatgc
     6721 cgtagggcaa ggcggtgcga tcgtcgcgtc cttctttgac gcccaagcga tacgcgagcc
     6781 aactgtagta cacgttgccg acaatcaagc tcagggcgac ggctggaagg atgcgtccgt
     6841 agaccagctc cgaagaatag cccagtagtc cccggcaaag tccaacgatc aggagaatct
     6901 gtatgagatt gtcgagcgcc aacccgagca ggccgtcgac gtcttgtcgc acgaaccagc
     6961 ggggccgatg cggctgagtg gcgtgagcat gatctgtttg cagcatgaga ggagttcgag
     7021 cggggctaca gtactcgaac cgaatgaaaa tgaaaagaag aagtatagga ggtcaatggt
     7081 gcctctgctc ccgcgatggg tgaaccgcag ggccctctcg ttgcttggct ccgcaaaagt
     7141 ggatgggtag gctaggtgct tccttgctcg ccgaacgcgc acaccaaacc aatcgtatgt
     7201 ttagggtggc ggtgcttgtc caagcgcgca gttggaagca acctagccca ccctgaggaa
     7261 ggacatacaa gcaagcttgg aagatcattg gaacggacct tcgaacactg cgtctcgcca
     7321 tcgccgcctg ggaaaagggc gcg
//
LOCUS       DASTQY010000283         3654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1614815, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000283 DASTQY010000000
VERSION     DASTQY010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614815"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1527)
                     /gene="zwf"
                     /locus_tag="VFR82_09450"
     CDS             complement(61..1527)
                     /gene="zwf"
                     /locus_tag="VFR82_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU4501864.1"
                     /translation="MAEPSLEPHLFIILGATGDLTRRKLLPALFHLRTYGELEKQNTV
                     IVGAAQPEMSEEAFRLWAYEGLNNSVARHAPDLRKWCHEYLHYQTVRRGGPEDYQQLA
                     QYIRQLEVDRNLPENRVFYLALPPTVVPPTVEFLDQAGLLKSRGWVRVVFEKPFGHDF
                     HSARALNTLLHRYLDESQIYRIDHYLGKETVQNLLAFRFANPIFESLWNRDSVENVQV
                     TVAEDIGVEHRGAYYQEAGALRDMVQNHLTQLVTVVAMEVPVSFEAGAIQAEKLKVLH
                     SISPIPLSEVVFGQYQAWQIADQRIPGYREEKGVLPDSMTETFVALRMEIHNWRWRGV
                     PFYLRTGKRLPRKITQVGVTFRPAPIQVFRSLEPGSLDANKLVITLQPSEGFALGFSV
                     KTPGRPFTFTGRALQFDYKQAFGGELPEAYETLLRDIMIGDQTLFVTADFTETAWRLY
                     DPLLAGKREVHPYTAGTWGPVEADQLLERYGHRWQLGW"
     gene            complement(1702..2100)
                     /locus_tag="VFR82_09455"
     CDS             complement(1702..2100)
                     /locus_tag="VFR82_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEU4501865.1"
                     /translation="MAEKLQVKTGSLKAVVNLTDRLQSLIGHAKMRDGLCTLFVTHTT
                     AALTTGEIGEGTEQDLLEVVQQVIPSIRFRHAHDPSHAWSHMAASILGPSLTIPVSDG
                     QLTLGTWQSVLLVELDGPRERTVYVTLLAS"
     gene            complement(2438..2797)
                     /locus_tag="VFR82_09460"
     CDS             complement(2438..2797)
                     /locus_tag="VFR82_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501866.1"
                     /translation="MGPTKAIIKDQALYEAATGKCIKDGFASRRDIEDYVNHHYLALP
                     VVDNAGNAWLLDGKPIYCFRGSQYETLDDQRVHLARCPDCGGMGIRSDEFTVESDCIR
                     CISCGHEFDARLEMMET"
     gene            complement(2892..>3654)
                     /locus_tag="VFR82_09465"
     CDS             complement(2892..>3654)
                     /locus_tag="VFR82_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4501867.1"
                     /translation="RIASKKPRPSLDRSIERMRKHLAELAVYVKKGKASRTLEEFDSD
                     SERLIAELLGETSALLDAYQYAQLGEAAGLVNMTEEAPESIGLDSERQSLLQRTRVLE
                     SCVSELEARRADEPKKFQSGRHMLTGPQVAEHMSSDLRSVPQTATLREAGRLMQKWKT
                     GSLLVTDSQTYVGFITDSALARDVVANGLDANTTPVQSCVRKPLVAIHGDRPLIEAVR
                     LMKEQATRHLAVAQDGEIIGVISVSNILRYYSGVV"
BASE COUNT          771 a         1075 c         1018 g          790 t
ORIGIN      
        1 caagcgatcc atccgctgtg gaatatcggt cacgatgaag gggggcggtt cgaccatcat
       61 tcaccatccc aattgccagc gatgaccgta tcgctccagc agttggtccg cctcgaccgg
      121 tccccatgtg ccggccgtgt acggatgcac ctcgcgcttg ccggccaaca gagggtcgta
      181 cagtcgccaa gccgtttccg tgaaatcggc cgttacgaat agggtttgat cgccgatcat
      241 aatgtcccgc agcaacgtct cgtaggcctc aggcaattct cccccgaacg cctgcttgta
      301 atcgaactgc agtgcacggc ccgtaaaggt gaagggacgg ccaggcgtct tgacggaaaa
      361 cccgagtgcg aatccttcac tgggttgaag cgtaatcacc agcttattgg catccagact
      421 gcccggctcc agagagcgga agacctggat gggcgccggt cgaaacgtaa cgccgacttg
      481 cgtgattttg cggggcagtc gttttccagt tcgcaggtaa aacgggacgc ctcgccagcg
      541 ccagttgtgg atttccattc gcagtgccac aaacgtttca gtcatcgagt ccgggagcac
      601 tcctttctct tctcgatagc cgggaatcct ctgatcggcg atctgccagg cctggtattg
      661 accgaagacg acttcgctca atggaatggg agaaatcgaa tgcaatacct tgagtttctc
      721 cgcttggatc gccccggctt caaacgacac cggcacttcc atggcgacca cggtgaccaa
      781 ctgcgtgaga tgattttgca ccatgtcgcg taaggcaccc gcctcttgat agtacgcgcc
      841 gcgatgctcg actccgatat cctcggcgac cgtcacctgc acgttctcca cagagtcccg
      901 attccacagc gattcaaaga tgggatttgc gaaacgaaac gccaacagat tttggaccgt
      961 ctctttgccc aaatagtggt cgatccgata gatctgagat tcatctagat aacgatgcag
     1021 gagcgtattc aatgcgcggg cagaatggaa gtcgtgaccg aacggcttct cgaagaccac
     1081 ccgcacccac ccccgacttt ttaagagacc cgcctgatcg agaaattcga ccgttggcgg
     1141 taccaccgtc ggagggagcg ccagatagaa gacccggttt tccgggagat tgcggtccac
     1201 ttccagttgt cgaatgtatt gggcgagttg ctggtaatct tcgggcccac cgcggcggac
     1261 ggtctgatag tgaagatatt catggcacca tttacgcaga tccggcgcgt gccgagcgac
     1321 ggaattgttg aggccttcgt aggcccagag gcggaaagcc tcctcgctca tttcgggctg
     1381 cgcggccccg acaatgaccg tgttttgttt ttccaattcc ccgtacgtcc gaagatgaaa
     1441 taaggccggc aacagcttgc gcctcgtcag atcgccggtc gcgccgagaa tgataaaaag
     1501 atgcggttca agcgaaggtt cagccatcgt ctccctttca cgtctacggg tctctttgac
     1561 cgtacgattt agtggttgat caggtcaacc caccaaaccc ctgaacgtcc tagatggatc
     1621 ttaccgaagg aaccggacca atggagggag tgaagcgcgt atgtggcgga gaaacaaccc
     1681 acagggtgac gtcacgcgga atcaagaggc gagtaacgtc acatagacgg tgcgttcacg
     1741 tgggccgtct aattcgacca acagcacgga ttgccatgtg cccaaagtca attgtccgtc
     1801 cgacacagga atcgtgagcg aaggaccaag aatcgacgcc gccatgtgag accaggcatg
     1861 cgacggatca tgcgcatgac ggaagcggat ggaaggtatc acctgctgca ccacctcgag
     1921 caaatcctgc tcagtcccct cgccaatctc ccctgtcgtc aaggccgcgg tggtatgggt
     1981 gacaaagagc gtgcacaagc cgtcccgcat ttttgcatga ccgatcagcg attgaagccg
     2041 atcggtgaga ttgacgactg ctttcagact cccagtcttg acctggagct tctccgccat
     2101 tcccttcatc cgttcgccag atcatgatca tcgacaagcg ggctcggaag aagaagcccc
     2161 caacgcacgc aatcaacgag tcataggttt actggagtca caagagtggg ccaggcactt
     2221 taccgctgaa ggcgtgaggg cgcccgatgc tggtccgact gcgcccgcgg gctcggctct
     2281 atcacactgc tccctccaag ctcgctcgtt tctcctccta agggcggtct ggatctatcc
     2341 ctcactgcgc gcttgaccga gcaccgcctt tctgaaaatc tgattggtcc ggtgtgccgc
     2401 ggtcggcgag caagagggat agactagacc gctcttctca cgtttccatc atctccaacc
     2461 gtgcatcgaa ctcgtgtccg caggagatgc aacgaatgca atcggattcc accgtaaatt
     2521 cgtccgaccg gatccccatg cctccgcagt ccggacagcg ggccagatgc acccgttgat
     2581 cgtcgagcgt ttcgtactga ctgccccgga aacaatagat tggtttgccg tccaagagcc
     2641 acgcgttgcc tgcattatcg acgacgggta gcgccaggta atgatggttg acgtagtctt
     2701 cgatgtcccg gcggctggca aagccatcct tgatacattt tcccgtcgcg gcttcatata
     2761 aagcctgatc tttgataatt gctttggtag gtcccatgag ctttcctcct gctttagaga
     2821 gttcttgctc ttctgcgtac atttctcgcg gtgccgaacg cttcgagcct cgttcccacg
     2881 agggcacgaa ttcaaacgac gccggagtaa taccgcagaa tgttggacac ggaaatcacg
     2941 ccgatgatct ccccgtcttg ggcgacagcg agatgcctcg tggcctgctc cttcatcaac
     3001 cgcaccgcct caatgagcgg ccggtctccg tgaatcgcca cgagcggctt ccgcacacag
     3061 gactgtaccg gggtggtgtt ggcgtctaga ccattcgcga cgacgtcacg ggccagagcg
     3121 gagtcggtga tgaagccgac ataggtttga ctgtctgtca cgagaagcga gccggttttc
     3181 catttctgca tcaaccggcc ggcttcccgc aaggtggcgg tttgcgggac gctacgcaaa
     3241 tcagatgaca tgtgctcggc aacctgaggg cccgtcagca tgtgtctgcc tgactggaac
     3301 ttcttcggtt cgtcggcgcg gcgggcctcc agttctgaga cacagctttc caggacgcga
     3361 gtgcgctgta gaagactttg gcgctcgcta tcgaggccga tgctttccgg cgcttcctcc
     3421 gtcatattga cgagtcccgc ggcctcgcct aactgcgcat actggtaggc atcgagcagc
     3481 gcagatgttt cgccgagcaa ttcggcgatc aagcgttccg aatccgaatc gaattcttca
     3541 agcgtgcggc tggccttgcc cttcttcaca tagacggcca gctccgcaag gtgtttgcgc
     3601 atgcgctcga tgctacggtc gagggacggt cgcggtttct tggaagcgat gcgt
//
LOCUS       DASTQY010000284         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1207678, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000284 DASTQY010000000
VERSION     DASTQY010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207678"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFR82_09470"
     CDS             <1..312
                     /locus_tag="VFR82_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501868.1"
                     /translation="HDASKAQQQMQALNDPNQQQAEHQTAAPSKDPSQLKDVVGSTDQ
                     AANKDIARGSGFSVGGSGGCQFKQGDMVRVEASDMGTITTIKALTDHPGSAIAGKGQE
                     Q"
     gene            complement(726..>2464)
                     /locus_tag="VFR82_09475"
     CDS             complement(726..>2464)
                     /locus_tag="VFR82_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4501869.1"
                     /translation="RAWPFIAPFRGQLACLCLVVLLSIPLSLLTPLPLTLAVDSVLGT
                     KPLPALLQAWLPTNVSSSSGSLLFFVCAAYVGIALLIHVQSMVLWLLSSYTGERLIYA
                     FRQRLFEHLQRICASYHEAHGPTDSVYRIQHDAASVKQIPIDAFLPFVKACCLLSGLA
                     ALMLIIDVEFAAVGLTMLPLLFWLTHRCGRRLRRQWTDVKSTETATVACAQEVLGASR
                     LVKAFGREEHEQRRFLGHAMNWVRQHNALATICSGFDFTFGMTITIGTAIALFIGISH
                     VKAGRLSLGDLLLLMAYMAQLAAPLDTAAKKLTELQSHLVGFRRALAILDTPPLVTDR
                     PASTPLNKAKGHVAFRGVSFTYPSSAAVLRDVSFHIPAGTRVGIVGPSGAGKSTIVNL
                     LTRFHDPTSGAVFLDGNDLREYRLTDLRKQFAIVPQDPQLFSTTIAENIRYGNLLATD
                     MQVEAAAKAAHATGFIRSLPQGYETPVGERGSRLSGGERQRIALARAFLRDAPIVILD
                     EPTSALDAGIEKDLIEVMERLTAGRTTLMIAHRLNTLRHCDFQLVLHQGRVIVQAPDW
                     ALQQPAGGLVSV"
BASE COUNT          607 a          670 c          755 g          432 t
ORIGIN      
        1 cacgatgcct caaaggctca gcagcagatg caggcgttga acgatccaaa ccaacaacag
       61 gccgagcatc aaacggccgc gccgtccaag gatccatcac aattgaaaga cgttgtcggg
      121 tcgaccgatc aggcggccaa caaggacatc gctcgtggtt ccggattttc ggtcgggggc
      181 tcaggcggat gtcaatttaa gcaaggcgac atggtgcgag tggaagcctc tgacatggga
      241 acgatcacca ccatcaaagc cttgacagac cacccagggt ccgctattgc aggaaagggc
      301 caagaacaat aatctcaacc acagatctca aggtgagacc tcatgtgtag gtctcacctt
      361 ggaaactctg aaacgagtga agggaaactg ggggtaagga tgactgaacc agagagcatg
      421 actcaggcaa ggggcaagag cgggaaagga aaagccgtat ttaaacgcag caagtcagaa
      481 gcgacgtccg aaacggcgtc tcgagaacgg caggaacctg gcaggagtcc gtcaacggac
      541 ccacgtcggg aggagacgtc gagtggtcaa gatccgcaaa tcatcgcacg aatccggcag
      601 cgcgcctact tgttgtatga agcgagcgga ttcgaagagg gccgcgctct ggaacactgg
      661 ctggaagcgg agcgacaggt gactggctca ccccagagac cgatagagaa gccgtcgaca
      721 gagacttaga ccgacaccaa gccgccggct ggttgctgga gtgcccaatc aggcgcctgg
      781 acaatgacac gtccctggtg caagacgagc tgaaagtcgc aatgacgcaa ggtgttcagc
      841 cgatgggcga tcatcagcgt cgtgcgcccg gcggtcagtc gttccatcac ttcgattaaa
      901 tccttctcaa ttcccgcatc cagcgcgctc gtcggctcgt caagaatcac gatgggtgca
      961 tcgcgcaaga acgctcgcgc aagcgcgatc cgctggcgtt cgcctccgga gagccgagag
     1021 ccacgctcgc ctaccggcgt ctcatagcct tgaggcaatg accgaataaa tccggtggca
     1081 tgagcggctt tggctgcggc ctcaacctgc atgtccgtcg caagcaggtt gccgtaacgg
     1141 atgttttcgg cgattgtcgt ggaaaacaat tgcgggtcct gagggacgat cgcaaattgc
     1201 ttgcggaggt cggtcaaccg gtactctcgc aggtcgttgc cgtccaggaa cacggcgccg
     1261 gaggtcggat catggaatcg agtgaggaga ttgacgatcg ttgatttgcc agcgccactc
     1321 gggcccacaa tccccactct ggtgccggca ggaatatgaa aagatacgtc ccggagcacg
     1381 gcggctgaag agggataggt aaaagacacg ccgcgaaacg ctacgtgccc tttggccttg
     1441 ttcaatggtg tggaggcagg ccggtctgtg accagtggcg gcgtgtcgag aatggcgagc
     1501 gcccgtcgaa atcccaccag gtgggattgt aattcagtca attttttggc cgcggtgtcc
     1561 aacggggcgg ccagctgcgc catataggcc atcagcagca gtaggtcacc caagctcaaa
     1621 cgtccggcct tcacgtggct gatgccgatg aacagcgcga tggcggttcc gattgtgatt
     1681 gtcatcccga acgtgaaatc aaatccgctg cagatggtcg cgagagcatt gtgttgccgt
     1741 acccagttca ttgcgtgacc gagaaaccgc ctctgctcat gttcttctcg tccgaacgct
     1801 ttgaccagac gcgaagcgcc caagacctcc tgggcgcacg ctactgtcgc tgtctccgtc
     1861 gacttgacgt cggtccattg ccgccgcaaa cgccggccgc agcggtgggt gagccagaaa
     1921 agcagcggca gcatcgtcaa accgaccgca gcgaattcca catcgatgat caacatcaac
     1981 gccgccagtc cgctcaagag gcagcaggcc ttgacgaacg gcaaaaacgc atcaatggga
     2041 atttgtttga ctgaggccgc gtcatgctga atgcggtaga ccgagtcggt ggggccatgt
     2101 gcctcatggt aagaggcgca gatgcgctga agatgttcaa acaatcgctg acgaaaggca
     2161 tagatcagtc gctcaccggt ataggatgac agaagccaca gcaccatgct ctggacgtga
     2221 atcagcaggg cgatgccgac gtaggccgcg catacgaaaa acagcaagct tcccgacgat
     2281 gaagagacgt tggtggggag ccaagcttgc agcaaggccg gaagaggctt ggtcccgagg
     2341 acgctatcaa ctgccaaggt aaggggcaag ggtgtcaata ggcttagggg aatggacagt
     2401 aggacgacga gacaaaggca agccagttgt cctcgaaatg gcgcgatgaa tggccatgcc
     2461 cgtt
//
LOCUS       DASTQY010000285         3821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1118432, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000285 DASTQY010000000
VERSION     DASTQY010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118432"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VFR82_09480"
     CDS             complement(<1..957)
                     /locus_tag="VFR82_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU4501870.1"
                     /translation="MTAPETSASSHRALHPWWQSAVFYQIYPWSFQDSNHDGIGDLRG
                     IQSRLEYLRSNTAASLGIDAIWLSPVYPSPMRDFGYDVSDYVNIDPRFGTLNDFDALV
                     QEAHQRGIRVIMDLVLNHTSDQHEWFRDSRRSRSASKRDWYCWADGKAFGRPPSNWNA
                     RFGGSSWCWDAGSSQYYLHSFLKEQPDLNWQNVAVRAAMWDVVKFWLDRGVDGFRLDA
                     INWLGKDMRWPDNPFRFGLRGYTRQHHIHDRDKEVAHQIMRELREVVSAYRDVVLIGE
                     AAADTPGGPASFYGTGDDELHLVFDFRVMKSPWGAESFRRFLS"
     gene            complement(1086..1814)
                     /locus_tag="VFR82_09485"
     CDS             complement(1086..1814)
                     /locus_tag="VFR82_09485"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEU4501871.1"
                     /translation="MLAIMIYAALSFTWGRAQDHEQERETSRLLAILFDSGRLVVGMN
                     QELINDASKGDKGFTPDVFEKQLRTVFEQRTGINLSDSDGKIPAIARPLLDRLVDESK
                     RTIAGYQTPINIPGIRYKGLIPATFGTETAARFTTWSGIYLRQIAPERFLRNKKNQPD
                     KYEADVLKALAEQASTSDESRPNWQVTDGGKTLRLVLPLYYSKACLDCHGEPKGQRDI
                     SGYPREGGKEGELGGAISVKLPIK"
     gene            2211..2507
                     /locus_tag="VFR82_09490"
     CDS             2211..2507
                     /locus_tag="VFR82_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501872.1"
                     /translation="MDDEMKALVQQYIDRRKNQRQDLVKELGLSEVEADQAVSTLELY
                     FHIEASEDQWAQAWTAAKRDFLTDGERTGVRLADLVVKYVDIARLGGGPESRWT"
     gene            complement(2704..3282)
                     /locus_tag="VFR82_09495"
     CDS             complement(2704..3282)
                     /locus_tag="VFR82_09495"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU4501873.1"
                     /translation="MKPTHHTSTIWRSSGGFTLIELTICLAIVAVLATLALPIHLEAV
                     EGAKAVEATSALTEVVRLERLRFAETGTYTADLQELGFQSVHSLQYTELFVEVYKDAN
                     GWSYRAFAMPKGRQHSNQIWGVTSRADGSFDMNFPGTLKSGGGSACSVWSGWGSMEGG
                     RIEGEETLSASSSTGGSGSPCGGRRVVDHGKK"
     gene            complement(3348..>3821)
                     /locus_tag="VFR82_09500"
     CDS             complement(3348..>3821)
                     /locus_tag="VFR82_09500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU4501874.1"
                     /translation="MHFALLFVDLDGFKPINDRLGHKAGDAVLRQTARVFQGCLRKGD
                     IVARYGGDEFTFLLNHVKGPSDAIRVAERILAKVQTPIDVGEPVQVGASIGIALSTNL
                     HERPEDLIRDADGAMYRAKAQGKQCFVLSDQAADVSTNELKARWKRLTHLGWNRV"
BASE COUNT          852 a         1018 c         1066 g          885 t
ORIGIN      
        1 tgacaggaac cgacggaagc tttcggcacc ccacggggac ttcatgaccc gaaaatcaaa
       61 cacgagatgg agttcgtcgt ctcccgtgcc gtagaacgag gctggtcccc cgggagtgtc
      121 ggcagctgct tctccgatca acacaacgtc tcgataggcc gatacgacct cgcgtaactc
      181 gcgcatgatc tggtgcgcga cttccttgtc ccgatcatga atgtggtgct gtcttgtgta
      241 gccgcgaagg ccgaaccgaa acggattatc gggccaccgc atgtccttgc ccagccaatt
      301 gatcgcatca agccgaaacc catccacccc gcgatcgagc cagaatttga ccacgtccca
      361 catggccgca cggacggcga cattctgcca gttgagatcc ggttgttcct tcaggaagga
      421 atggagatag tattggctgc ttccagcatc ccaacaccag gatgagccgc cgaatctggc
      481 attccagtta cttggtggcc gcccgaaggc cttgccgtct gcccaacaat accaatcgcg
      541 tttggaggcg gaacgggacc tccgcgagtc gcggaaccac tcatgctgat cggaggtgtg
      601 gttcagcacg agatccatga tgacgcggat tccccgttgg tgagcctcct gcaccagcgc
      661 gtcgaagtcg ttgagcgtgc cgaaccgtgg gtcaatgttg acgtaatcac tgacatcgta
      721 gccgaaatct cgcattggag aagggtagac gggcgagagc cagatggcgt cgatgccgag
      781 cgatgcagcg gtgttgctgc ggaggtactc cagccttgat tgaatgcctc gcagatcgcc
      841 aatgccatca tgattggagt cctgaaaact ccatggatag atctgataaa aaacggccga
      901 ttgccaccag ggatgaagcg cacgatggga cgaagcggat gtttctggcg cggtcataga
      961 gacaaggcag agtctctacc ctttggagag agtattgtca aatttgttaa tcactttgcg
     1021 gcgttcccgg agagtctatc attccagggt gagtgaggcg gtagtgcttg gtgcgattgc
     1081 gttgattact ttatcgggag tttcacgctg attgctcccc caagctcacc ctcctttccg
     1141 ccctcgcgcg ggtatcccga aatatcacgc tggccttttg gctcaccgtg gcaatcgaga
     1201 caggccttgc tgtaatacaa tggcagcacg agacggagag tcttgccgcc atctgtgacc
     1261 tgccagtttg gccgtgattc gtcagaggtc gaggcctgtt cggccagggc tttgaggaca
     1321 tcagcttcat acttatcggg ttggttcttc ttgtttctca agaatctttc cggtgcgatt
     1381 tgtcgaaggt agatgccgga ccatgtcgta aagcgggcgg cagtctccgt gccaaaagtc
     1441 gccggaatga gtcccttata gcgaattcct ggaatattga tgggggtttg gtacccggca
     1501 atagttctct tactttcatc tacaagacga tcaaggagag gacgggcaat agccggaatc
     1561 ttaccgtccg agtccgacag attgatgccc gttcgttgtt caaagacagt gcggagctgc
     1621 ttttcaaaaa catcgggagt aaaacctttg tctcccttgg aggcatcgtt gataagctcc
     1681 tggttcattc cgactacaag acggccggaa tcaaacagga tggccaaaag acgtgaagtt
     1741 tctcgctcct gctcatgatc ctgtgcacgt ccccaggtga aggaaagggc ggcgtatatc
     1801 atgatcgcca gcatggccac catgatgaag cgaggcgatc gaagtcttaa caggtgtgtg
     1861 aaatgaggca tgaaatgggt ggcttttctt tccgcgcttt ttcaagctgg ttgcgccggt
     1921 gggccattct agctatggcc ctggttttag cgcaagagca gattgtcgtt cagggatagc
     1981 agagggataa gtatgagcga tctcattacg tgggttggag ggaaggtctg cggccttctg
     2041 caagttatct gacgaagagc ctcgattctc cattgcttag gctgttttcc cagattgtga
     2101 attcattcga agcgaggtac acagacgatc agacatcgaa tggtccgata cattcgtctt
     2161 gccgcaggat acgatccttg catcagagac aggcagcaaa aggaggggtg atggatgatg
     2221 agatgaaggc cctcgtgcag cagtacattg atcggaggaa aaatcagaga caggacctcg
     2281 taaaggagtt gggactctcc gaggtggaag cagaccaggc cgtctccaca ctagaattgt
     2341 atttccacat cgaggcatct gaggatcagt gggcacaggc ttggacagcg gccaaacgcg
     2401 attttctgac cgatggcgag cgcactggtg tacggctggc tgatcttgtc gtcaaatatg
     2461 tcgacatcgc acgcctgggt ggagggccag agagtcgatg gacgtaaaga atcggtgagg
     2521 tttcgacaag aagagaaggg tttattcgcc gggctccacg aagtgtggct tggtgatcgt
     2581 gacccggatc gaacgggtga gccgtggaac tatcccatgc gtgggcttca ttggatgttt
     2641 agctaaaacc agtgagagag aagcaaactc attaaccgta ggtcaacaag gcgtcactca
     2701 cttttatttt ttcccgtgat ccaccaccct ccttcctcca caaggcgacc ccgatccgcc
     2761 tgttgaggaa ctggcactta gcgtttcttc gccctcaatt cgccccccct ccatggatcc
     2821 ccagccgctc cagacgctac aagcggatcc accgccggat ttcaatgttc ctgggaagtt
     2881 catgtcgaaa ctaccatcag cccgactcgt cacaccccaa atttggttag agtgttgtct
     2941 acctttgggc atggcaaagg ctcgataact ccagccattg gcatctttgt atacttcaac
     3001 aaagagctca gtgtattgga gggaatgaac cgactggaat cccaattctt ggagatcggc
     3061 ggtgtatgtt ccagtctctg caaaacgcaa gcgctccagt cgaaccacct cagtcagggc
     3121 tgacgtggcc tcgacagctt tggctccctc cacggcctcc agatgaatcg ggagagccag
     3181 ggtggccagc accgcaacaa tggccagaca gatggtcaac tcgatcaggg tgaatccgcc
     3241 actgctccgc catattgtgg atgtgtggtg agtcggtttc attggtggtt ccatgctgtt
     3301 tctgttgcgg tcttcgaaac cgcaatgaat tcttcgggtc ctcctgccta gacccggttc
     3361 catcccaggt gcgtgagtct cttccaccgc gctttgagtt cattggttga cacgtccgcg
     3421 gcttgatcgc tgaggacaaa acactgtttg ccttgcgcct tcgcccggta catggcgccg
     3481 tcagcgtcac gaatcaaatc ttcaggacgt tcgtgcaggt tcgtgctcag ggcaatgccg
     3541 atgctcgcgc cgacctgtac cggttcaccg acatcaatcg gcgtctgcac tttggcgagg
     3601 atgcgctccg ccacacgtat cgcatccgag ggacctttga cgtggttcag taagaaggtg
     3661 aattcgtctc caccatagcg tgcaacgatg tcgcccttcc tcaaacaccc ttgaaacact
     3721 cgtgccgtct gtcggagcac ggcgtcgccg gctttatgtc ccagccggtc attgatcggc
     3781 ttgaacccat ccaagtcgac aaacaagagc gcaaagtgca t
//
LOCUS       DASTQY010000286         2700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_625643, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000286 DASTQY010000000
VERSION     DASTQY010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625643"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VFR82_09505"
     CDS             complement(<1..815)
                     /locus_tag="VFR82_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HEU4501875.1"
                     /translation="MAKQELLVEVKKLISIGKEKGFLTYDELNSTLPAEVVSSEQFGS
                     IMTMFGEMDIEIVDAPDGERHQKNRSAAPDQEEAGEDTEDSEAEDNEKEIDLTPGALS
                     RTDDPVRLYLKEMGSVALLSREGEIEIAKRIEEGKRDIAFVIYGMPMTIEFILALRDQ
                     LKNGKIDVREIVPIQETEEDFEEEEVERDYEELRLKTLEGLNTVRKVSTSLKGLYEKS
                     RQAGKDPAKLKKIKKQIDAIREQVVDKMEAVNLHGVLKDRMVQRVREMAIQIRT"
     gene            complement(818..2641)
                     /gene="dnaG"
                     /locus_tag="VFR82_09510"
     CDS             complement(818..2641)
                     /gene="dnaG"
                     /locus_tag="VFR82_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU4501876.1"
                     /translation="MGRGLISDDVINQIKDRIDIAEIVGQHVSLTRAGQNLKGLCPFH
                     QEKTPSFTVSSSRQIFHCFGCGAGGNVFTFLTRITGASFPETVRDLGRKVGIEVQDIV
                     SASGPQIAQGPRLEQLNQMAAQWFQQNLQNDRSGAEARAYLAGRGILEATIERFGIGV
                     APAEWDGLIRWLGKQGYTQQDMAAAGLVIARDNGTGYYDRFRSRVMFTITDLRKRVVG
                     FGGRVLGDGTPKYLNSPDTPLFKKGQTLFALDVAREAVSRTKTVIVVEGYFDAIALHQ
                     AGLTHTVATLGTALTLEHVQVLRRFASKVVLLFDPDAAGVRAALRGLDLFVNSGLGVK
                     VVTLPAGEDPDTYVRKEGIDAFARLEQQAPSLLDFALEQSVKTAESSTMEGRIQGVDE
                     ILRILQKSEHPIEREERIRLVAERFGINQQRLIDRYPALREKHPPRSAGSKSEAPPVI
                     SSKGAPEERDLIYLLLHGQLSAADVRRLRPEAFSMPACRRIVETALVHLEPDGRVGLR
                     RLLDVVVDDPDCGPLATELSMQEQHFDDARAHIQGCLETIDRKRAEAVLRDLITQLKA
                     AEREGRIEDARVLNARVNDMRLQKAGRPVGVLSMVKGVSKE"
BASE COUNT          526 a          837 c          710 g          627 t
ORIGIN      
        1 gtccggatct ggatcgccat ctcacggacc cgctggacca ttcgatcctt caggaccccg
       61 tgcaggttca cggcttccat tttgtcgaca acctgttcac ggatcgcgtc gatctgcttt
      121 ttgatcttct tgagctttgc gggatctttt cccgcctggc gggacttttc gtacagcccc
      181 ttcagcgacg tcgacacctt ccggacggta ttcaagcctt ccagggtctt gagccgcaat
      241 tcctcgtagt cccgctcgac ttcctcttct tcgaagtcct cttccgtttc ttgtatcggc
      301 acgatctcgc ggacgtcgat cttgccgttc ttaagctgat cgcggagcgc gaggatgaat
      361 tcgatcgtca tcggcatgcc gtagatgaca aatgcgatgt cccgttttcc ctcctcgatg
      421 cgcttggcga tttcaatctc gccttcgcgg ctgagcagtg caacgctccc catctccttg
      481 aggtagagcc tcaccggatc gtccgtccga ctcagtgccc ccggtgtcag gtcaatttcc
      541 ttttcattgt cctccgcttc actgtcctcc gtgtcttcgc cggcctcctc ctgatcgggg
      601 gcggcactcc ggtttttttg atgacgttca ccgtccgggg catcgacgat ttcgatgtcc
      661 atttcgccaa acatcgtcat aatgctgccg aactgctcgg aagagaccac ttcggcgggc
      721 aacgtgctgt tcaactcgtc gtaagtcagg aaccccttct ccttaccgat cgagatcagc
      781 ttctttactt ccacgagcaa ttcttgtttc gccatgctta ctccttactt actcccttca
      841 ccatagaaag cactccgaca ggacgaccgg ccttttgcag acgcatgtca ttgacacggg
      901 cattcaacac cctggcatct tcgatccgtc cttcacgctc tgcggccttg agctgcgtga
      961 tcaggtcacg cagcacggct tctgcccgct ttcgatcgat cgtctcgaga cacccctgta
     1021 tatgcgcgcg ggcgtcgtca aaatgttgtt cttgcataga caattctgtg gccaggggcc
     1081 cacagtccgg gtcatcgacg acaacatcga gcaatcgacg aagtcctacg cgaccgtccg
     1141 gctcaagatg gaccagtgcg gtttcaacga ttcggcggca ggctggcatc gaaaacgctt
     1201 caggacgcaa acgacggacg tcggcggcgg acaactggcc atgcagcaac agatagatca
     1261 agtcccgttc ctccggagcc cccttcgacg agatcacggg cggtgcctcg cttttggacc
     1321 cagccgaacg cggcggatgt ttttcccgca aggccggata ccgatcaatg agccgctgct
     1381 gattgatgcc gaagcgttcg gcgaccagac gaatccgttc ttcccgttcg atgggatgct
     1441 cgctcttttg caagatgcgc aagatttcgt ccacgccttg gatccgccct tccatggtac
     1501 tgctctccgc agtcttgacg ctctgctcaa gcgcaaaatc cagtaaactc ggagcctgct
     1561 gttccaggcg ggcaaaggcg tctattcctt cttttcgaac gtaggtatcg ggatcttcac
     1621 cggccggcaa tgtcaccacc ttcacgccca atccgctgtt gacgaagaga tccaaccctc
     1681 gcaaggcagc ccggacaccg gccgcatcgg ggtcgaacag caacacgact ttcgaggcga
     1741 accgccggag cacctgcaca tgttcgaggg tgagggcagt cccaagggtc gccactgtgt
     1801 gagtcaaccc cgcctggtgc agagcgatcg catcgaaata gccttcgacg acaatgaccg
     1861 tcttcgtgcg gctgacggcc tcgcgggcga catcaagggc aaagagcgtt tgccctttct
     1921 tgaacagcgg cgtatccggc gaattcaaat atttaggtgt cccgtcgcca aggacgcggc
     1981 caccgaatcc gacgacgcgc ttgcggaggt cggtgatggt gaacatgact cgactgcgga
     2041 aacggtcgta atacccggta ccattgtccc gagcaattac caagccggct gcggccatgt
     2101 cttgctgggt gtacccctgc ttgccgagcc acctgattaa tccatcccat tccgcggggg
     2161 cgacaccgat gccgaatcgc tcgatcgtcg cctccagaat gcctcgccct gccagatagg
     2221 ctcgcgcctc agctccggag cgatcgttct gtaaattttg ctgaaaccat tgtgcagcca
     2281 tctggttgag ttgttcgagg cgagggcctt gagcgatctg gggtcctgaa gcggagacga
     2341 tgtcctgaac ctcaatcccg actttgcgac cgagatccct gaccgtttct ggaaaacttg
     2401 ctccggtaat tctggtcaaa aacgtgaaca cattgcctcc cgcgccacag ccaaaacagt
     2461 gaaagatctg ccggctggag ctgacggtga acgaaggagt tttttcttgg tggaaggggc
     2521 aaagaccctt caaattctgt ccggcacggg tcaagctgac atgctgccca acaatctcgg
     2581 cgatgtcgat ccgatctttg atctgattga tgacatcgtc agaaatcagg cctcgaccca
     2641 tgggagacgc agctcctcac tcccccaaac gcggtggggc tggtatgcgt aagtgatcgg
//
LOCUS       DASTQY010000287         3724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_886499, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000287 DASTQY010000000
VERSION     DASTQY010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886499"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VFR82_09515"
     CDS             <1..726
                     /locus_tag="VFR82_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEU4501877.1"
                     /translation="VIRQIVHEITAVSAALKVPLPLDMPDRVVKWTQEIRDIHTSMYD
                     DWKAGRQTEVRNLNGYIVERGRELGIPTPVNEALTAMIKTITEKPRTGPGIIEIDGAV
                     VQPVAFDRMALRQLPAECQVNDVNTRMSSMRGRAVTVKGLLEIPALAIAADHVTFHSH
                     DGKYAATLTLQQARDFGLLLYEVDDQPLPEAKGGPYRLITPGMGDLCANVKAVRRIEV
                     RIGSGKDTRPSERPSVCTEDKRS"
     gene            complement(727..2052)
                     /locus_tag="VFR82_09520"
     CDS             complement(727..2052)
                     /locus_tag="VFR82_09520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU4501878.1"
                     /translation="MANGVTQIRRSVLALALPVTVSSLLQRTEGIVAVFLVGGLGAIP
                     IAAVGLGQLLAFIASTLVSGLSVGTNVIVAQLWGARRHHDAGQAARHFLALSLAVSLV
                     LMAGGLLLNRLAMRLLGAAPEVIALSLPYSTLIFLVIPFTVLLQVLSSILQGTGDTKT
                     PMYAMIVVNVLHILIAYPLIYGHWGFPAIGVQGAAIAVGIAESAGTLYLGLRCLPLLH
                     RSQHVRWDLFRTMWQVGAPVSGERIVQQAGILAYTKIVLLYGTISYAAHQVGLSIESL
                     SFLPGYGFAIAAATMVGQSIGAGKYTRAKLENWEANRLAIVIMSGMGLVFFFFPYALL
                     RAFTSDESVIELGTLFLKIVALIQIPLALTMVLAGSLRGAGDTHFIMVATTIGMWAIR
                     VPIALITGVWLHLGIFYVWLAMIADWTLRMGLMLWRYRSERWKAIQVIR"
     gene            2125..3675
                     /locus_tag="VFR82_09525"
     CDS             2125..3675
                     /locus_tag="VFR82_09525"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HEU4501879.1"
                     /translation="MTTSPEPDHLPLIAPRLSKSKFLSGLQCHKRLYLEIMRPELATP
                     PDPSTQAILAMGTEIGVLAQRRFPGGVVVGPAVRQREAAILQTAKLIEDSRIPAIFEG
                     AFEYEGVVVRVDILERISVADGTSPAWRLIEVKSSSRVKDVHLDDLAIQRHVLLGVGL
                     NVAETCLMHIDTGYLYQGGEIDLQALFAVDEVSALVIDRQRKVPEQLIAMKAVLSEPH
                     PPAIEPDARCHSPYECRFWVHCTQDKSPRWIYRLPGAKKVVEGLVQNGITTIDDIPDD
                     VNLSITQRRVKDNVEWVSTGLGSMLRAVAYPIHHLDFETVMLAVPRFPAMRPYQSIPV
                     QWSNHIELESGQIIHQEFIHGDATEPRKRWAETLIESLGDAGTIVVYSSYEEAMMRQV
                     AEAFPEFQRALSAITRRLWDLLPVIREHYYHPGFQGSYSIKFVLPAIVPGMSYRDLLI
                     QDGGQAASEYYRMVFVEEDWIEQAKIREALLQYCARDTMAMVELRRVLMEKAAPLSSQ
                     AGSKVELS"
BASE COUNT          866 a         1005 c         1134 g          719 t
ORIGIN      
        1 gtcattagac agattgttca tgaaattacg gccgtgtccg ccgcgctcaa agtgccgctg
       61 cccctggaca tgccggatcg ggtggtgaag tggacccaag agattcgaga cattcacacc
      121 tcgatgtacg acgattggaa agccggacgg caaaccgagg tcaggaattt aaacggctac
      181 atcgtggagc ggggccgtga attgggaatt ccgacaccgg tcaacgaagc gttgacagcg
      241 atgatcaaga caataacgga gaagccgcgg accggtccgg gcatcatcga gatcgacggc
      301 gcggtcgttc agccggtggc ctttgaccga atggcgctgc gtcagctgcc ggctgagtgt
      361 caggtcaatg acgtcaatac gcgcatgtcc agcatgagag gacgggccgt tacagtgaag
      421 ggcttgctgg aaattcctgc gctggccatc gccgccgatc acgtgacctt tcattcgcat
      481 gacgggaagt acgccgcgac gctgacgctt caacaggcca gggactttgg gttgctattg
      541 tatgaagtgg atgatcagcc actgcccgag gcaaagggcg ggccgtaccg cttgatcact
      601 ccgggcatgg gcgatctctg tgccaatgtc aaagcagtcc gtcgtatcga agtgcggatc
      661 gggtccggga aagatacccg cccgtccgaa cggccgtccg tctgtaccga agacaagcgc
      721 tcctgattat cgaatcacct gaatggcctt ccatcgttcc gatctataac gccacaacat
      781 caagcccatt ctcagcgtcc agtctgcaat catggccagc cacacgtaga agatgcccaa
      841 gtggagccag acccctgtga tcaaggcaat gggaacccgg atagcccaca tgccgatggt
      901 ggttgcgacc atgatgaaat gcgtgtcgcc ggcgccgcgc agtgagccgg ccagcaccat
      961 ggtcagagcg agcggaattt ggatgagggc gacgatcttt aaaaagaggg tgcccagctc
     1021 gatcactgat tcatcgctcg tgaacgcgcg gaggagcgcg taggggaaaa agaaaaacac
     1081 caagcccatt cccgacatga tcacgatcgc cagccggttg gcctcccagt tctcgagctt
     1141 cgctctcgta tattttccgg cgccgatgct ttgacctacc atcgttgcgg cggcgatggc
     1201 gaatccataa ccggggagga atgacaagga ttcgattgac aggccgacct gatgagcggc
     1261 ataggagatc gtgccgtaga gcagcacgat tttggtgtag gcgagaatcc cggcctgctg
     1321 aacgatgcgt tcgccggaga cgggcgcacc aacctgccac atggttcgaa agagatccca
     1381 ccggacgtgc tgcgagcgat gcagcagggg aagacagcga aggcccaaat acaaggtgcc
     1441 ggcgctctcc gcgatgccga cggcgatggc tgcgccttgc acgccaatcg ccgggaaacc
     1501 ccaatggccg taaatcaagg gataggcgat gaggatgtgc aggacgttca caacgatcat
     1561 ggcatacatc ggcgttttcg tgtccccggt gccttggagg attgaagaca gcacttggag
     1621 caacacggtg aacggaatga ccagaaagat gagcgtcgaa tagggcagtg aaagtgcgat
     1681 gacctccgga gcggctccca ggagccgcat ggccaagcgg ttcagcaaga ggcccccggc
     1741 catcaagacg agcgacacgg cgagtgagag ggcgaggaaa tgacgtgcgg cctgacccgc
     1801 gtcatgatgc cgacgggcac cccacaattg cgcgacgatg acgttggtcc cgaccgagag
     1861 cccggagacc aaggtgctgg cgataaaagc caaaagctgg ccgagaccca ccgcggcaat
     1921 cggaatggcg cccaacccac cgacaagaaa cacggcgaca atcccttccg tgcgttggag
     1981 cagactgctg acagtgacgg gaagtgccag tgccaggacg gagcgccgga tctgagtgac
     2041 accattggcc atcaacccat cctaacagaa gcggtactga gcggcctgga caaacaaggc
     2101 gagcatcaat tagggtttgc tgacatgacg acatctcctg agccggacca cttgcctctc
     2161 atcgcacctc gcctttccaa atcgaaattc ctgtcaggat tgcaatgtca caagcggttg
     2221 tatctcgaaa tcatgcggcc tgaactggcc acgcctcccg atccttcgac gcaagccatc
     2281 cttgccatgg gaacggaaat tggtgtgctg gcacagcgac gattccctgg cggagttgtg
     2341 gtcgggccag cggttcggca acgagaagcg gcgatcctcc aaactgccaa gctgatcgaa
     2401 gactctcgga tacccgccat tttcgaaggc gcgttcgaat atgaaggggt cgtggtgcgt
     2461 gttgatattc tcgaacggat ctctgtcgcg gacgggacat ctccggcttg gcggctgatc
     2521 gaagtgaaat cgtccagcag agtgaaggat gtgcacctcg atgatctcgc gattcaacga
     2581 cacgtgctac tgggggtcgg cctcaatgtg gccgagacct gcctcatgca tatcgacacc
     2641 ggttatctgt accagggcgg tgaaatcgat ctgcaggctc tgtttgccgt cgacgaggtg
     2701 tcggcgctcg tcatcgatcg gcaaagaaag gtgcctgagc aactcatcgc aatgaaggcc
     2761 gtgttgtccg aaccgcaccc gccggcgatt gagccggacg cccgttgtca tagcccctat
     2821 gaatgccgat tttgggttca ctgcacacaa gacaagtctc ctcggtggat ttatcgctta
     2881 cccggtgcga aaaaggtcgt ggagggatta gtccaaaacg gtattacgac aatcgacgac
     2941 attcccgatg acgtgaattt gtccatcacc cagcgtcggg tgaaagacaa tgtggaatgg
     3001 gtctcgaccg ggcttggtag catgctaagg gccgtggctt atcccattca tcatctcgac
     3061 tttgaaaccg tcatgttggc cgttccgagg tttcctgcga tgaggccgta ccagtcgata
     3121 ccggtgcaat ggtccaatca cattgagctc gaatccggtc agatcattca tcaggagttc
     3181 attcatggag acgcaacgga accacgtaaa cgatgggcgg agacgttaat tgaatcgctc
     3241 ggcgatgcgg gcacgatcgt ggtgtactcc tcctatgaag aagcgatgat gcgacaagtg
     3301 gcggaagcct ttcccgaatt tcagcgggcg ttgtccgcaa ttaccaggcg gttgtgggat
     3361 ctgcttcctg tcatcaggga gcattattat catcccgggt ttcagggctc ctactcgatc
     3421 aaattcgtat tgccggcgat tgtgccaggg atgagttatc gagacctctt gattcaggac
     3481 ggcgggcagg cggccagtga gtactatcgg atggtctttg tcgaagaaga ctggatcgaa
     3541 caggcgaaaa tcagagaagc gctgctgcaa tattgtgcga gggatacgat ggcgatggtc
     3601 gaactgcggc gtgtgttgat ggaaaaggcg gcgccattgt cctcgcaggc cggcagcaag
     3661 gtcgagctga gctaaaaagg atggagtggt tgggaagggc cagttccgac tcgccggcgt
     3721 ccgc
//
LOCUS       DASTQY010000288        11169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1230172, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000288 DASTQY010000000
VERSION     DASTQY010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11169
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230172"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /gene="ispG"
                     /locus_tag="VFR82_09530"
                     /gene_synonym="gcpE"
     CDS             complement(<1..774)
                     /gene="ispG"
                     /locus_tag="VFR82_09530"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEU4501880.1"
                     /translation="MHISRRKTRQIQVGTVKIGGDAAISVQSMCSTDTRDVAATVEQI
                     RHLESAGCELIRVAVPDDEAAAALPKIKAAMTVPLIADIHFDHRLALKAAEIVDCVRI
                     NPGNIGAWWKVEEVIKAVNARGIPLRVGVNGGSLERPLLDKYGWPSPEALAESALNAV
                     HALEDVGFTNLKVSLKASDVHHAIDAYWLFAHQSNYPLHIGITEAGTAMTGAVKSSIG
                     LGYLLSQGIGDTLRVSLAADPVEEVKVGFEILKSLELRHR"
     gene            complement(931..2880)
                     /gene="dxs"
                     /locus_tag="VFR82_09535"
     CDS             complement(931..2880)
                     /gene="dxs"
                     /locus_tag="VFR82_09535"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEU4501881.1"
                     /translation="MSILKTIHGPADLKRLKPAQFLKLCQELREQIIGAVSNVGGHLA
                     SNLGVVELTVALHYLLDTPQDRIVWDTSNQAYAHKLLTGRREQFHTLRQYGGLSGFCK
                     REESVYDTFNAGHAGTGVSAAYGMVEAREQQGQKHKVVCVVGDGAMTAGMTLEGLHHA
                     GGLGKDFLVVLNDNQMSISKNVGAISSYLNRTFTGEFFTKMREETGQLLRKIPHIGYD
                     VEKLARRAEELAKGAILPGLLFEELGFRYAGPIDGHNFEHLLPTLENVLKMRGPVLLH
                     VITKKGLGYAPAEKNPVWFHACPPFVRETGAPAKKAVRPSYTQIAIDSLIRLAREDRR
                     LVAITAAMCEGTGLNAFEKEFPDRLYDVGIAEQHAVTFAAGLAAQGMRPVVAMYATFL
                     QRAYDQVVHDVATQNLPVTFCIDRGGLVAEDGTTHHGAFDYAYLRHMPNMVVMAPKDE
                     NELQHMLKTALHYDGPASVRYPRGVSLGVKMDRELSELPIGKAELLRDGTDVAIIAIG
                     VSVWPAVKAAEQLSQEGLSTAVVNARFAKPLDQELIVEVAKRVRYIVTVEEGCRMGGF
                     GSAVLEALSDAGVTGVRTKILGLPDWYIEQGPQDLLRERYGLTTDGIARSVKELVSKS
                     PAVSREAEYFSAAIDHLPHGDEQGS"
     gene            complement(3063..4091)
                     /locus_tag="VFR82_09540"
     CDS             complement(3063..4091)
                     /locus_tag="VFR82_09540"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HEU4501882.1"
                     /translation="MLCSDDRRGGSTCRALVAGLIGVACLHPSSASAEGFGPFPVRNF
                     NPLQQLVLNMPGDRAAVLEPGALDVRLELAETAAIYSEAPPGAAASVKFETLRNGLFL
                     RYGATRKLEVALEVPVLYRYTGFMSGAIEAVERATTGISPARQALSQVSYAYSISRNG
                     QQIVTGTKGAVGLGDSTLQAKYQLLTETSSMPALSLRTAIKLPTGNEREFFGSGSPDF
                     GVGVAAEKRLGGRWILYGNLSTVLPTGRIAGLSLYPTISGMAATEYLWSDNLSLTLQF
                     DYYTTPFRHVGLEVFDKGATELVAGFSYRMAEHWLWQMYAVENVDFITGGAADFTVAV
                     LFTYRYRT"
     gene            complement(4093..5295)
                     /locus_tag="VFR82_09545"
     CDS             complement(4093..5295)
                     /locus_tag="VFR82_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEU4501883.1"
                     /translation="MTTPRGIAIFLVCLAVGMTAVWVRLAAADTQIFPIPSASTSKND
                     GSDAGLIVPILITDPDGELKYLLAPMVIKNSIVGTRGTFNLFRYDTGGRQMQFIASLT
                     EKIERKLVFNYIDPAFSDGRYFLNFGGSFFKNATSRFFGLGQTTVEADETNYTAREAR
                     ANWRFGVYANEVTQIAVGQRVRQVSLQRGATDLPFTGDRYPTVDGVQGETIIIGHRAT
                     FYYDTRNNLISPTDGMAVTAYAEVNQNLRNSDFPVFSRYELEVKKLFPSESKRAILIV
                     RADLQATLGSQVPFFEQSSLGGQNNLRGYGVDRFIDKHLIAASVEERIHLVRAKIAGV
                     AADFEMATFLDTGQVFTDFKDVSFKDYRMTPGIGFRGIVRPNVVGRIDYGYSKEGGAI
                     FAGLDFPY"
     gene            complement(5316..5957)
                     /locus_tag="VFR82_09550"
     CDS             complement(5316..5957)
                     /locus_tag="VFR82_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4501884.1"
                     /translation="MRMSAPVQRNFGRTVCAGLMVAALGLAGVVPDTARAGAATEAMK
                     GTIEEVLKILADKDLKQPAKANERRQLLEKVVGERFDYQEMSRRSLGAPWANLSDKDK
                     QEFVSLFQTLLVNTYADKVESYSGEGVQYVNERTEKEYAEVRTKVLTGKTEIPLDYRL
                     LNKGSTWRVYDVVVDGVSLVNNYRGQFSKILRNGSYAELIEQLRKKSDKIKAP"
     gene            complement(6009..6452)
                     /gene="mlaD"
                     /locus_tag="VFR82_09555"
     CDS             complement(6009..6452)
                     /gene="mlaD"
                     /locus_tag="VFR82_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEU4501885.1"
                     /translation="MEKTKLEFIVGVFVLVGIVCLGYLSIKLGKLELIGGDVYEVIAQ
                     FNSASGLKAGSAIEIAGVEVGRVRAIRLNEDRAAVVLAVDKTVKLYTDTIASIKTRGI
                     IGEKFLALSPGGGGDPLKPGDTIRDTESGLDLEELVSQYVHGNVK"
     gene            complement(6472..7227)
                     /locus_tag="VFR82_09560"
     CDS             complement(6472..7227)
                     /locus_tag="VFR82_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4501886.1"
                     /translation="MIRLVGVEKTLGGQPVLQGMDFSVPAGKLTTVIGKSGEGKSVLL
                     KHMIGLLQPDRGQVWVGDVEISRLRGKALNEVRKRFAMLFQGAALFDSLSVFENVAFP
                     LREKLRLKGTEVARRVDEKLEQVGLAGMGHKFPAELSGGMKKRAGLARALVMEPEIML
                     FDEPTTGLDPLMAKSIHDLIVTMQRRFKFTAVMVSHEIPEIFAISDWVAMLRKGKIAL
                     MAPAADFVKTTDPEIQEFISVSGPIRLSGVAAG"
     gene            complement(7231..7995)
                     /locus_tag="VFR82_09565"
     CDS             complement(7231..7995)
                     /locus_tag="VFR82_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4501887.1"
                     /translation="MMVKRLGAWALDSIQEMGRMLLFVLSSFVWLVRPPLRFVQIVKQ
                     LHFVGYKSTFVVVLTAGFTGMVLALQGYYTLRKFGSEGLLGSAVALSMIRELGPVLAA
                     LMVTARAGSAMTAEIGIMRITEQIDALDTMAINPLQYLIAPKLVAGLVGVPLLVAIFD
                     VVGIYGGYLVGVELLGGNGGAYWNSIESAVEWKDVYGGILKSISFGLIVSWVCCYKGF
                     FTKHSAEGLGTATTEAVVLSSVLILVWDYFLTSVLL"
     gene            complement(7992..8840)
                     /locus_tag="VFR82_09570"
     CDS             complement(7992..8840)
                     /locus_tag="VFR82_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501888.1"
                     /translation="MLTQGRPTSFTDGHSLGGSAASPVALFVATRWELAAVRRALPVD
                     HRIDIGGVGCFIGRQAARSYWLIHTGIGPEAAHAAVSAVLNREPMALAISVGFAGALA
                     PGAAIGDVIVGTSVVSGTFDDGWHESGQPVLCHEGAADAARAAAADIGSKAHRGALVS
                     VATVICRAAEKQRIARLTGAIAVDMESAALGTAALRRGVPFAVCRTVSDIVTEDLPLD
                     FNLFLTRSGWMRGITAVVMDPSSLIGLNRLRRQSRVAADRLTALCSAYAARGFGLAPA
                     TNIGRA"
     gene            complement(8834..11005)
                     /gene="shc"
                     /locus_tag="VFR82_09575"
     CDS             complement(8834..11005)
                     /gene="shc"
                     /locus_tag="VFR82_09575"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEU4501889.1"
                     /translation="MPMNLIRTILDRLSDSLFISVPDHLTADPLAGPSLRLVSDKSTV
                     VADATSRRLSSQAGSQLDTIDDAIRRSQAWFLSRQHPTEGYWVAELEADTTLTAEYLM
                     LRRLLDQVDPERERKAVSYLRAAQLPDGGWPIYYGGPSEISASVKAYFALKLSGIAGD
                     EPFMLRARNCILGKGGLVQANVFTKITLALFDQYDWEGIPHMPVELMFLPKWFYFNIY
                     AISYWSRAVLIPLLIVFAHRPVCRISKEQGIEELYASPRAEIRYWKFPPFNKDQKWLT
                     PHNVFVALDALLKIYDHMPLLWLREKALHRAATWMLEHIKGSGGLGAIYPAMANSIMA
                     LQCLGYQVDDPLVRKALREIEDLEVYDTVSIGDQRVEAMHLQPCHSPIWDTSLLMNAF
                     IEAGMPQDHPALQRGAAYLMSRQTKTVGDWKVSSPNAEPGGWYFQHENELYPDVDDSA
                     VVLMALAKVKMADPAELKDSIRRGAAWVVAMQGSDGGWGAYDKDNNRVVFNYIPFADH
                     HALLDPSTADLTGRCLEMLAALGYDRSHPAVAPALAFLKHEQEPDGSWYGRWGVNYIY
                     GTWSVLAGLRAIGEDLSRPYIRRAVAWLESKQNPDGGWGESCLSYADPDCAGRGPSAP
                     SQTAWALLGLMSAGMCDSFSVARGIQFLLRHQMKDGSWEEVRHTGTGFPRVFYLRYHW
                     YCQYFPLWALAMYRNLRTRNKMRADEVRNQILAAGCYRFDC"
BASE COUNT         2391 a         3409 c         3065 g         2304 t
ORIGIN      
        1 gcgatggcgc agctccagcg atttgagaat ttcaaacccg actttcactt cttccaccgg
       61 atctgccgcc aacgacacac gcagcgtgtc gccgattccc tgggagagca ggtagccaag
      121 gccgatagac gatttcacgg caccggtcat cgccgtcccg gcctccgtga tgccgatatg
      181 caacggataa ttggattgat gggcgaaaag ccagtaggca tcgatcgcgt gatgcacgtc
      241 ggacgccttg agcgacacct ttagattggt aaaccccacg tcttccaatg cgtgcaccgc
      301 attgagcgcc gattcggcaa gcgcttcggg ggacggccag ccatacttgt cgagcagcgg
      361 gcgctcgagc gatccgccgt tcacaccgac ccgcagcgga atgccacgtg cattcaccgc
      421 cttaatgacc tcttcaacct tccaccacgc accgatgttg ccgggattga tgcggacaca
      481 atcaacgatt tctgcggcct tcaaggccaa ccgatggtcg aagtgaatgt cggcgatcaa
      541 aggaacggtc atagccgcct tgatctttgg aagcgcggct gccgcctcgt catccggaac
      601 ggcaacgcga atcagctcgc agcccgcgct ctcgagatgt cggatctgct cgaccgtcgc
      661 cgcgacgtcc ctcgtatcgg tcgagcacat cgactgaact gagatcgcgg cgtcgccgcc
      721 gatctttacc gtgccgactt ggatctgcct ggttttacga cgtgagatgt gcatcgtagt
      781 cgatagtgaa cagagcgccc atcttcgatt ctttcgctta aactcttaac atgccaaggc
      841 ccgcgccacg accgatcgct taccgcggct tgccggcggc tgctgtgaca gcgcgccaac
      901 ttcaggcttt aacatatccc ctctccctgc ttagctcccc tgttcatccc cgtgcggcag
      961 gtgatcaatt gccgctgaaa agtattctgc ttcgcgcgac acggcggggc tcttgctgac
     1021 cagttccttg acgctacggg cgattccgtc cgtcgtcaat ccatagcgct cgcgcaagag
     1081 atcttgcggc ccttgttcga tgtaccaatc tggcagaccc aggatcttcg ttctgacgcc
     1141 ggtcacaccg gcatcggaga gtgcctcgag cactgccgag ccgaaaccgc ccatcctgca
     1201 gccttcttcg acggtcacaa tgtagcggac gcgcttggcc acctccacga taagctcttg
     1261 atccaagggc ttggcaaagc gggcattaac caccgccgtc gacaacccct cctgactgag
     1321 ctgttctgcc gccttcaccg ccggccacac ggacacgcca atagcaatga tggcgacatc
     1381 ggtgccatcg cgcaggagct cagccttgcc gatgggcagt tctgagagtt cccggtccat
     1441 cttcacaccc aggctcactc ctcgtggata ccgaacagag gccggaccgt catagtggag
     1501 cgcggtcttg agcatatgct gaagctcatt ttcatctttg ggggccatga cgaccatgtt
     1561 gggcatgtgg cgcaaatagg cataatcgaa tgcgccatga tgcgtcgtgc cgtcttccgc
     1621 gacgagcccg ccgcggtcaa tgcagaacgt cactggaaga ttctgggtgg caacgtcgtg
     1681 gaccacttga tcgtacgcgc gttgcaggaa tgtcgcatac atcgccacga ccggccgcat
     1741 gccctgcgcg gccaagccgg ctgcaaaggt cacggcatgc tgctcggcga tgccgacatc
     1801 atagaggcgg tcggggaatt ccttttcaaa ggcattcaag cctgttccct cgcacatcgc
     1861 tgcggtaatg gcgacgagcc gacggtcctc ccgggctagc cggatgagcg agtcgatagc
     1921 gatctgagta taggagggtc gtacggcttt ctttgccgga gcccctgttt cccgaacaaa
     1981 gggcggacag gcatggaacc acaccgggtt cttttctgcc ggcgcatagc ctaagccctt
     2041 tttcgtgatc acgtgaagta ggactggtcc ccgcatcttg agcacatttt ccagtgtcgg
     2101 aagcagatgc tcgaagttgt gcccgtcgat cggaccggca tagcggaatc ccaattcttc
     2161 gaacagcaag cccggcagaa tcgctccctt ggccaattct tctgcacgac gggcgagctt
     2221 ttcgacgtca tagccgatat gcgggatctt ccggagcagc tggccggttt cttcacgcat
     2281 cttggtaaaa aactctccgg tgaacgttcg gttcaggtag gaagagatgg cccccacgtt
     2341 cttggaaatc gacatctgat tgtcgttcaa aaccacgaga aagtctttgc cgaggcctcc
     2401 ggcatggtgc aacccttcga gggtcattcc ggccgtcatc gcgccgtcgc cgaccacgca
     2461 gaccactttg tgcttctgac cttgctgctc cctcgcctcg accatcccgt aggcggccga
     2521 gacgccggtg ccggcatggc ccgcattgaa agtatcgtag acgctctctt cacgcttgca
     2581 aaacccgctg agcccgccat attgccggag ggtatgaaac tgctcccgcc gcccggtcag
     2641 cagcttatgg gcatacgcct ggttgctcgt gtcccacaca atccggtctt gaggcgtatc
     2701 gagcaggtaa tggagcgcca cggttaattc aacgacccct agatttgagg ctagatggcc
     2761 gcccacattg gatacggccc cgatgatctg ctctctaagc tcttggcata atttaagaaa
     2821 ttgcgcaggc ttcagccgct tgagatcagc cgggccgtga atggttttca agatcgacat
     2881 ggcaaaactc ctttgcgagt gtcctgcccc agtatcagcg ggccgagttc agataggctt
     2941 ggcgaagagt gaagcggtta gcataccggc tcgagcagtt tcacacaaga cctccatgaa
     3001 gtcaagcggt taggccattt tcttccttgg ggtaggtcct ttccccttat ggacgacgcc
     3061 catcatgttc ggtaccggta ggtaaagagg actgccacgg tgaagtccgc ggcccctccg
     3121 gtaatgaaat cgacgttttc gacggcatac atctgccaga gccaatgctc ggccatgcga
     3181 tagctgaagc cggccacgag ttccgtggct cccttgtcga aaacctcgag acctacatga
     3241 cggaaggggg tggtgtaata gtcgaactgt agcgtcagtg acaggttgtc cgaccacaga
     3301 tattcagtgg ccgccatccc ggagatcgtc ggataaaggg acaatccggc gatacggccc
     3361 gtcggcagga cggtgctcaa gttgccgtat agtatccatc gccctccgag ccgcttctcg
     3421 gctgcgactc caaccccgaa atccggactg ccgcttccaa aaaattcccg ctcgttgcct
     3481 gtggggagtt tgattgccgt ccgaagcgac agcgccggca tcgaggacgt ttcggtcaaa
     3541 agctgatact tcgcctggag cgtgctatcg ccgagtccga ccgctccctt ggttccagtg
     3601 acgatctgtt gcccattgcg tgaaatacta taggcgtagc tgacttggct gagcgcctgg
     3661 cgtgccggcg atatgccggt cgttgctcgt tcgacggctt caatcgcccc cgacatgaaa
     3721 cccgtgtacc ggtacaacac gggcacttcg agagcgacct ccaacttacg ggtcgcgcca
     3781 tagcgcaaaa aaaggccatt ccgcagcgtt tcaaatttta cgctggctgc ggcgccgggc
     3841 ggggcttcgc tgtaaatggc agcagtttca gcgagctcga ggcgcacgtc caatgcgccg
     3901 ggctccaata ctgccgcccg gtcacccggc atattgagga ccagctgctg cagaggattg
     3961 aaattccgca caggaaaggg gccgaagcct tcagcggacg ctgaggacgg gtgcaggcag
     4021 gccacgccga tgaggccggc cacaagcgcc cggcaggttg aaccccctcg gcgatcatcg
     4081 gagcaaagca tgtcagtatg ggaaatccag gccggcgaag atggcgcctc cctctttgct
     4141 atatccatag tcgattcgac cgacgacatt gggtcggacg atgccccgga acccgatacc
     4201 aggcgtcatg cggtagtcct taaaactgac gtccttaaaa tcggtaaaca cctgacccgt
     4261 gtccaagaag gtggccattt caaaatccgc tgccacgccg gcgatctttg ctctgacgag
     4321 atgtatacgt tcttccacgc tggccgcgat cagatgctta tcgataaaac gatcgacgcc
     4381 gtagccgcgg agattgtttt gtcctccgag cgaactctgt tcaaaaaacg gcacctgtga
     4441 gccgagggtc gcctgcaggt cggctcggac gatcaggatg gcgcgcttgg attcgctggg
     4501 gaagagcttt tttacctcca actcataccg tgaaaacacc gggaaatcgc tgttcctcag
     4561 gttctgattg acttccgcat aagccgtcac ggccatgccg tccgtcggcg agatgaggtt
     4621 gttcctcgta tcgtagtaga aggtcgcccg gtgcccgatg atgattgttt ccccttgcac
     4681 gccgtccacc gtggggtatc gatcccccgt aaagggaaga tcggtggcgc ctctctgcag
     4741 gctgacttgg cgcacgcgtt ggccgacggc gatttgcgtc acctcattgg cgtagacgcc
     4801 aaaccgccag ttcgcccgcg cttcgcgcgc ggtatagttg gtttcatccg cctcgacggt
     4861 ggtttgcccg agcccgaaga atcgagaggt cgcatttttg aagaacgaac cgccgaaatt
     4921 taagaagtac cggccgtcgc tgaaggccgg atcaatgtag ttgaagacca acttgcgctc
     4981 aatcttctca gtgagcgacg cgataaactg catctgcctg ccaccggtgt cgtaacgaaa
     5041 cagattgaat gtccctcgcg tgccaacgat cgagttctta atcaccatcg gggccaacaa
     5101 atacttcaac tctccgtctg gatcggtgat gaggatgggg acgatcaatc cggcgtcgct
     5161 gccgtcattt tttgacgttg acgcggacgg gatcgggaat atctgggtat ccgctgcggc
     5221 gagtctcacc cacacagcgg tcattcctac ggccaggcat acaagaaaga tcgcgatgcc
     5281 gcgcggggtc gtcatgaagg atcggctcga aggtgttacg gggctttgat tttgtcggat
     5341 ttcttgcgga gttgctcgat caattcggca tagctgccgt tacgaaggat tttgctgaac
     5401 tgtcctcggt aattgttcac caggctgacg ccgtccacga ccacatcgta gacccgccag
     5461 gtggaaccct tgttgaggag acgataatcc agtggaatct cggtcttccc tgtgaggacc
     5521 ttcgtccgga cctcggcata ttccttttcg gtgcgttcgt tcacatactg cacgccctcc
     5581 ccggaatagg attcgacctt gtcggcgtag gtgttgacga gcagagtctg gaagagcgag
     5641 acaaactcct gcttgtcttt gtctgacaga ttcgcccagg gcgctcccaa tgaccgacgg
     5701 gacatttcct gataatcaaa gcgctctccc accacctttt ccaataattg tcggcgctcg
     5761 ttcgctttgg ccggctgctt caggtcttta tcggccagga ttttcagcac ttcttcgatg
     5821 gtgcccttca tagcctcggt cgccgcgccg gcccgggcgg tgtccgggac cacgccagcc
     5881 agtcccagag ctgccaccat cagccctgca catacggttc ggccgaaatt cctctgaacg
     5941 ggtgcggaca ttctcatagc tcgactccct catgctcgta tgtctcgatc cggcgcaacg
     6001 gcgccaggtc atttcacatt accatggacg tactgactga ccaactcttc taaatccaag
     6061 ccggactccg tgtcccgaat ggtatcgccg ggttttaacg gatccccccc tccacccggt
     6121 gaaagggcaa gaaatttctc cccgataatg ccacgtgttt tgattgacgc gatcgtgtcc
     6181 gtatagagct tgacagtttt gtccactgcc agaaccacgg ccgctcgatc ctcgttgagt
     6241 cgaatcgccc ggacgcggcc gacttccacg ccagctattt cgatcgccga gccggccttc
     6301 aggcccgagg cgctattgaa ctgggcgatg acttcataga cgtcgccacc gatcagttcg
     6361 agcttaccga gcttaatcga gagatacccg agacagacaa tgcccacaag cacgaacacg
     6421 ccaacgatga attccagttt ggtcttttcc atccgtgcac tccttgccgt ctcagcctgc
     6481 ggctactccg ctcagcctga tcggaccgct cacagaaata aactcctgga tttcaggatc
     6541 agttgtcttc acgaagtcgg cggccggcgc catgagggca atcttgccct tgcgcaacat
     6601 cgccacccaa tcactgatgg cgaaaatttc ggggatctca tggctcacca tcacggcggt
     6661 aaacttgaac cgccgctgca tcgtgacaat taaatcgtga atcgacttcg ccatcaacgg
     6721 atccaggccg gtggtgggct cgtcaaagag catgatttcc ggctccatga ccagcgcacg
     6781 ggcgaggccc gcccgtttct tcatgccgcc gctcaattcg gccggaaact tatgccccat
     6841 cccggccaac cccacctgct cgagcttctc gtcaacccgc ctcgcgacct cggtcccctt
     6901 cagtctcaat ttctcccgca aagggaacgc gacattttcg aagacgctca gcgaatcgaa
     6961 caatgccgct ccttgaaaca gcatggcaaa ccgcttgcga acctcattga gggccttgcc
     7021 gcgcaaccgg ctgatctcga cgtcgccgac ccaaacttgt ccgcggtccg gctgcagcag
     7081 gccgatcata tgcttcaaca gcacgctttt cccctcaccg ctcttgccga tcacggtcgt
     7141 cagttttccg gccgggactg aaaaatccat gccctgtaac accggctgtc cgcccaaggt
     7201 tttttccact cccacgagtc taatcatgac ttacagcaag acagaggtga gaaagtaatc
     7261 ccagaccaag atcaataccg aggacagcac caccgcttcg gttgtggctg tgccgagtcc
     7321 ttcggcgctg tgcttggtaa aaaatccttt atagcagcag acccagctga caatgagccc
     7381 gaaactgatc gacttgagaa taccgccata cacatccttc cattccacag ccgattcgat
     7441 cgagttccaa taggccccgc cgttgccgcc caacagctcg actcccacta gatagccgcc
     7501 atagataccg acgacatcga aaatcgcgac gagcagcggc acgccgacca gacccgccac
     7561 gagtttggga gcaatcaagt actgcaacgg attaatcgcc atcgtatcca gcgcatcgat
     7621 ctgctccgtg atgcgcatga ttccgatttc agccgtcatg gcggaaccgg cccgagccgt
     7681 caccatcaac gctgccagca ccggtcctaa ctcccgaatc atgctcaagg cgaccgcaga
     7741 gcccagaaga ccttcggacc cgaactttct gagcgtgtag tagccctgca gtgcgaggac
     7801 catgccggta aagccggccg tgagcacgac aacgaaggtg gatttgtacc cgacgaaatg
     7861 cagttgtttg acaatctgca caaaccggag cggcggacgg accaaccaga caaacgatga
     7921 gagcacaaat aagagcatcc gccccatctc ttgaatcgaa tccaaggccc atgcgcctag
     7981 tcgtttgacc atcatgctct tccaatatta gtggctggcg ccaagccgaa ccctcgtgcc
     8041 gcataggcgg aacacaaggc cgtcaaccgg tcggcagcca cacggctctg gcgccgtagg
     8101 cggttcagtc caatcaggct tgatgggtcc atgacgactg cggtaatccc tcgcatccag
     8161 cccgatcgcg tgagaaacag attgaaatcc aggggcagat cttcggtgac gatgtctgac
     8221 acggtccggc aaacggcaaa gggaacgcct ctcctgaggg cggccgttcc tagcgccgcg
     8281 ctttccatgt ccacggcgat ggcgcccgtg agacgcgcga tgcgttgctt ctctgcggcc
     8341 cggcagatga ctgtcgccac agacactagg gcgccgcgat gcgctttcga tccaatgtcg
     8401 gcagccgccg ctcgtgccgc gtccgctgcc ccctcatggc agagcaccgg ctgtcccgac
     8461 tcgtgccagc cgtcgtcgaa cgtccctgag accacgctcg tgccaacgat cacgtcacca
     8521 atggcagcac ctggggccag cgcgccagca aagccgacgg aaattgccag ggccatgggt
     8581 tcgcgattca gcacggcact cacagcggca tgagccgctt caggcccgat tccggtgtga
     8641 atcagccaat acgaccgagc ggcctgccgg ccgatgaaac agcccacacc gccgatgtca
     8701 atccggtggt cgaccggtaa ggcacgccgc acggctgcca attcccatcg cgtcgccacg
     8761 aacagtgcga ccggactggc agcagatccg ccgagtgaat gtccgtcagt gaatgaggtc
     8821 ggccggcctt gagtcaacaa tcaaaccgat agcatcccgc cgccagaatc tggttccgca
     8881 cttcgtcagc tcgcatcttg ttgcgcgttc tcagattccg atacatcgcg agtgcccaca
     8941 gtggaaaata ctggcagtac cagtgataac ggagatagaa cacgcgtgga aacccggtgc
     9001 cggtgtgccg aacctcttcc cacgaaccat ccttcatttg atgacgcaga agaaattgga
     9061 tacctcgcgc cacactgaac gaatcgcaca tgccggccga catcagccca agaagcgccc
     9121 aggcagtttg cgacggggca cttggacccc tgccggcaca atcgggatcc gcataggaaa
     9181 gacacgactc tccccatccg ccgtccggat tctgcttcga ttcgagccaa gccaccgcac
     9241 gacgaatgta aggccgtgac agatcttctc caatggcgcg gaggcctgcg agaacggacc
     9301 acgtgccgta aatataattc actccccacc ggccgtacca actgccgtct ggttcttgtt
     9361 cgtgtttgag aaaggccagc gcgggcgcca ccgccgggtg cgaccgatca tatccaagag
     9421 cagcaagcat ttcgagacag cgtccggtca ggtccgccgt gctcggatcg agcaacgcat
     9481 gatggtcggc gaacgggatg taattgaaga caacgcgatt gttgtctttg tcgtacgcac
     9541 cccagccgcc gtccgagccc tgcatggcca cgacccaggc ggcccctcgt cgaattgagt
     9601 ccttcagctc cgcagggtct gccattttga cctttgcgag cgccattagc acgacggccg
     9661 aatcatccac gtcgggatac agttcattct cgtgctggaa ataccatcca ccaggttcag
     9721 cattgggaga ggacaccttc caatcgccga cagttttcgt ttgacgggac atcagatagg
     9781 ctgcgcctcg ctgcagcgcc gggtggtcct gtggcatccc cgcctcgatg aatgcattca
     9841 tcaacagcga agtatcccaa atgggcgagt gacaaggctg taagtgcatg gcctcaaccc
     9901 gttgatcacc gatcgacacc gtgtcgtaga cttccaggtc ttcgatttct ctgagcgctt
     9961 ttcggaccaa gggatcgtcg acctgatatc ccaaacactg gagcgccata attgagttgg
    10021 ccatagcagg ataaatcgcc ccgagaccac cgctgccttt gatatgttcc agcatccagg
    10081 tggcggcacg atgcagcgcc ttttcacgca accacagaag cggcatgtgg tcataaatct
    10141 tcagtaacgc atccaaggcg acgaatacat tatgaggcgt cagccacttt tggtctttgt
    10201 taaacggtgg aaatttccaa tagcgaattt cagcgcgggg gctcgcatag agttcctcga
    10261 tgccctgttc cttggaaatg cggcaaaccg gacgatgcgc gaagacgatc aacaggggaa
    10321 tcaacacggc gcgagaccaa tatgaaatcg cgtaaatatt gaaatagaac cacttgggaa
    10381 ggaacatgag ttccacgggc atgtgcggaa tgccttccca gtcgtactga tcgaagaggg
    10441 ccagcgtaat cttcgtaaag acattggcct gaacgaggcc gcctttgccg agaatgcaat
    10501 tgcgcgcgcg cagcatgaac ggttcgtctc ccgcaatgcc gctcaacttg agagcgaaat
    10561 acgctttcac cgacgcgctg atttccgatg gcccgccgta gtagatgggc caccctccgt
    10621 cgggcaattg ggcggcgcgc aaatagctga cggccttgcg ttcccgttca ggatcgactt
    10681 gatccagaag ccgtctcagc atcaggtact cagccgtgag ggtggtatcg gcttccagtt
    10741 cggcgaccca atagccttcg gtcggatgtt gtcgcgacag aaaccaggct tggcttcgcc
    10801 gtatggcatc atcgatggtg tcgagttggc tgcctgcctg agatgaaagg cggcgtgacg
    10861 tagcatcggc aacgacagtt gacttgtctg acaccagccg gagtgagggc ccggcaaggg
    10921 ggtcggcggt gaggtggtct ggaaccgaaa tgaacaagct atcggaaagg cggtccagga
    10981 tagttcgaat cagattcatc ggcattgaag gaacacaccg gcactgtgaa tgcgggtgat
    11041 cagcggtgcc actgacctcg cgacaagaga agccctggac cccatcgctg cgggactagt
    11101 ccgtggggat ataacagacg gtgtggtcga agtcaagcaa ggggatgcgg aagtcctctg
    11161 aactacggg
//
LOCUS       DASTQY010000289         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_138374, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000289 DASTQY010000000
VERSION     DASTQY010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138374"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2197
                     /locus_tag="VFR82_09580"
     CDS             <1..>2197
                     /locus_tag="VFR82_09580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEU4501890.1"
                     /translation="GALRGMVEAFQSQLDVIKQGGQEDPSVSAHWKKEIMTLLPVSSN
                     ASAEGSSEELGTDYLVPTIDAEVLSYFAPEADEYLQTLEVLTNRLKENPGDAETIQAL
                     YRTAHTLKGSAHTIGFKVIGDIAHPIESCMIAVRDGHIPVSTALLATIGDAATLIRLV
                     LRRDESTLAQLRQDVTGVTQTLHQIESGQPVGAPASAVARASEPPVAHVATAVLEPPA
                     ATDQPATAVAAPSLTDEYLMPQLDPEVLSYFAPEAQEYLESLEAQLLRLEKEPQNPDL
                     INQLFRTAHTLKGSAYTVGFQSIGDLTHYIEDFMGAVRESRVKVLPGHTDVLLRSVDV
                     VRLLMRRDPTLTGTVRQRFAAAMTALKQLEQTKTVETLVKAAPVQSKPEPVVEEAAQQ
                     PEPEQVKVRDAAAEEREVIRVSRDRLERLLNLVGELVIGRGRLEQRLSVLEQLSQQVL
                     ACRVRLIDSVRSFEEKHTFTLPTSTGGAADQSKGTPGAAMFQGLSDFGSLEFDKYDDF
                     NILARRISEVTADISESMTQLSGSIRRSHEDMSQLQQLTLGMRDEIARARMVPIGTPF
                     TRFRRATREMARATGKEVTLVTSGEHTEVDTGVVERLVDPLVHLVRNAVYHGIEPAAL
                     RVSKGKPAAGTIYLHASHRGNAVLIEVEDDGAGLDIDKIRAKAVERGLVRSDIAKNLP
                     EAEAIKFIFLPGFSTAEQVGDQAGRGVGMDVVKRVIESMNGHIDVESIRG"
BASE COUNT          452 a          646 c          673 g          426 t
ORIGIN      
        1 cggtgcattg cgcggcatgg ttgaagcctt tcaatcgcaa ctggatgtca tcaagcaggg
       61 gggccaggaa gatccgtccg tgagtgccca ttggaagaaa gagatcatga ctctgttgcc
      121 ggtttcctca aatgcctccg cggagggtag ttcggaagag ctgggaacag actacctggt
      181 cccgaccatc gatgcagaag tgctttccta ttttgcccct gaagccgacg aatatctcca
      241 gaccttggag gtcctgacca accgtctcaa agaaaatccc ggggacgcgg aaaccatcca
      301 ggcgctgtac cggaccgccc atacgcttaa agggtcagcg cacaccatcg ggtttaaggt
      361 catcggcgac atcgctcatc ctattgagtc ctgcatgatt gcggtgcgcg atggacacat
      421 tcccgtgtcg acggcgctcc tggcgacgat tggagatgct gcgaccctga ttcgcctggt
      481 gctgcgcagg gacgaaagca cgctcgcgca attgcgtcag gacgtcacgg gtgtcaccca
      541 gaccttgcac cagatcgaaa gcggtcagcc cgttggtgca ccagcgtcgg ccgtggcgcg
      601 tgcgtcggaa ccgcccgtcg cccacgtcgc gacggccgtg ctggagccgc cggctgccac
      661 ggatcagccc gctactgcgg tggctgcacc ctcgttgacc gatgagtatc tgatgccgca
      721 actcgacccg gaggtcttgt cctatttcgc gcctgaggcg caggagtatc tggagtcatt
      781 ggaagcgcag ttgcttcgcc ttgaaaaaga accgcagaat ccggatttga tcaatcaatt
      841 gtttcgcacc gcgcatacgc tgaaaggttc tgcctatacc gtcggcttcc agtcgatcgg
      901 cgacctgacg cattacatcg aagatttcat gggcgcagta cgtgaaagtc gggtcaaggt
      961 cctgccgggg cacaccgacg tattgctgag atcggtggac gtcgtgcgtc tattgatgcg
     1021 ccgtgatccc acgctgacgg gaactgttcg tcagcggttt gccgcggcga tgaccgcgct
     1081 caaacaactt gaacagacga aaacggtcga gactcttgtc aaggcggccc ccgttcagtc
     1141 aaagcccgag ccggtcgtcg aagaggctgc gcagcagcct gagcctgaac aggtcaaggt
     1201 gagagatgcc gccgccgagg agcgggaagt cattcgcgtc agccgtgatc gtttggaacg
     1261 gttgctcaac ctggtcggcg aattggtcat cggtcgtggg cggctcgagc aacgtctcag
     1321 tgtcttggaa caactttcac agcaagtgtt ggcctgccgc gtgcgactca tcgactcagt
     1381 acggtcgttc gaagaaaagc atacgttcac cctgccgacc tccactggcg gtgcggccga
     1441 tcagtcgaaa ggcacgccgg gagcggcgat gttccaggga ctgagcgatt tcggcagtct
     1501 cgaattcgac aagtatgacg acttcaacat cctggcccgg cgcatcagcg aagtcacggc
     1561 ggacatctcc gagtccatga cgcagttgag cggatccatc cggcggtcgc atgaggacat
     1621 gagccaactg cagcaactta cactcggcat gcgggatgag atcgcccgcg cgcgtatggt
     1681 gccgatcggc acgccgttca cacgcttccg ccgggcgact cgggaaatgg caagggcgac
     1741 gggtaaggaa gtcaccctgg tcacctccgg agaacacacg gaagtggaca ccggcgtcgt
     1801 cgagcgtctc gtcgatccgt tggtccatct ggttcgcaat gccgtctacc atgggatcga
     1861 gccggcggcg ttacgcgtct ccaagggaaa gcccgcggcc ggcacgatct atctccacgc
     1921 ctctcatcga ggcaatgccg ttctgatcga agtggaagac gatggggccg gtctcgatat
     1981 cgacaaaatc cgcgcgaagg cggtcgagcg cggccttgtg cgttctgaca tcgccaaaaa
     2041 ccttcccgaa gccgaggcca tcaagttcat ctttcttccc ggcttctcga ccgccgagca
     2101 ggtgggtgat caggcgggcc gtggcgtcgg catggacgtc gtcaaacggg tcatcgagag
     2161 catgaatggc catatcgacg tagaatccat ccgcggt
//
LOCUS       DASTQY010000290         4624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_143102, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000290 DASTQY010000000
VERSION     DASTQY010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143102"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFR82_09585"
     CDS             <1..370
                     /locus_tag="VFR82_09585"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEU4501891.1"
                     /translation="GAATRGNYVTLDASTTLGLFMRNGWPDEGLCLKVIGQIIQSAEG
                     SKPVRIYGEMVAVLWAEGNALAAIHLERLWNKLAAQRNFTLLCGYPSSAFHGADMDFA
                     FEDVCACHSQIKGFRSSARV"
     gene            complement(442..855)
                     /locus_tag="VFR82_09590"
     CDS             complement(442..855)
                     /locus_tag="VFR82_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4501892.1"
                     /translation="MATQAGGIRVLLVDDHRMVREAIRSLLKSVQSIDVVGEADNGAE
                     AIASIGKLSPQVVVMDINMPKMDGLVATKLVRRHYPNVAVLGLSVTEDSYHKSAMEKA
                     GAFRVMTKGTHGLDDLCLEIEKAAATMQFLSRARR"
     gene            1185..1892
                     /locus_tag="VFR82_09595"
     CDS             1185..1892
                     /locus_tag="VFR82_09595"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU4501893.1"
                     /translation="MNIHFVRFVVIAGVIVAIGGCAVSSDRPSGEEAQKASPTARPLD
                     PQQAERLKTVMIPLLAKMDKPLDPSQASVSFLDSPDINAANAGGGKFFVTRGLLEKAN
                     DEQLRGVMAHEIAHEDKGHVTELQTIGAGVGIASMLLNQINPIAGAIVPVAGDLLVAK
                     PHGRQAEYEADAHAVTILRRSGLDGKAIMTNTLTWLKQTAGSSKGGFFSTHLGSDDRI
                     QKIQEIAMGVVRTPPIP"
     gene            complement(2328..2513)
                     /locus_tag="VFR82_09600"
     CDS             complement(2328..2513)
                     /locus_tag="VFR82_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501894.1"
                     /translation="MARIDRAPTLVEQQPFFQDCLWVLNGAFVDGDRLRFELGVRALV
                     DFCNEITNTGDVEQWWD"
     gene            3291..3689
                     /locus_tag="VFR82_09605"
     CDS             3291..3689
                     /locus_tag="VFR82_09605"
                     /inference="COORDINATES: protein motif:HMM:NF018228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4501895.1"
                     /translation="MALLATVKRLLVVEDDPDQSRAVRERLELYGYAVSCAQDGQNAM
                     DMLQAEAAFDGVLLDLNLPHVPGLQVLAATRQALPHVPVLVMSASSSRLQAAKESNLA
                     CGYIAKPFGADQFKQAVRGCFGPAYQPTAL"
     gene            3721..>4624
                     /locus_tag="VFR82_09610"
     CDS             3721..>4624
                     /locus_tag="VFR82_09610"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEU4501896.1"
                     /translation="MEDDTKETHDEETFRLLFESIQDYAIFVMNPEGIVKTWNPGAEM
                     MKGYRSDEIVGQSFSRFYLPSDIEAGKPHMLLSHAARAGRVTDEGWRVRKDGSHFWAS
                     IVITALYDQKGAIKGYAKITQDMTERKQMEDDLQDTLFLLGQANKKLEELDQLKSLSI
                     SVATHELRSPLTAVKAYVDNLFEGVAGELPEKVFYYLTRIGYNTDRVIRLVNMLLDIS
                     RIEAGHMHLELDRVSVSEVIRDVVKDFECMARTKEIAIRASAVMDAPVRADRDRVEQV
                     LHNLLHNALKFTPPGGEIVVQSQLT"
BASE COUNT         1094 a         1227 c         1193 g         1110 t
ORIGIN      
        1 tggtgcagca acaaggggta attatgtgac cttggacgcc tcaacgacgt taggtctgtt
       61 catgcggaac ggttggccgg acgaggggtt gtgtttaaaa gtaatcggtc aaattatcca
      121 atccgcggag ggcagcaagc ccgtacgcat ttatggcgaa atggtcgcag tcctctgggc
      181 tgaagggaac gctcttgctg cgatacacct tgaaagactg tggaataaat tagcggctca
      241 gcggaacttt acgctgcttt gcgggtatcc ttcttccgcc tttcacgggg cagacatgga
      301 ttttgccttc gaggatgttt gtgcctgtca ttctcaaata aaagggttca gatcgtccgc
      361 ccgagtgtga tccctgccat aaccccgcca ggagctgctg gtgtcgatgc ctgcgaggac
      421 tctgtcagcc gcgacgcaaa tctatctcct cgcacgcgaa agaaattgca tggttgcggc
      481 agccttttca atttctagac ataagtcatc taagccgtgg gtgccctttg tcatcacccg
      541 gaaggcccct gctttctcca tcgccgattt gtggtagcta tcttctgtga cggataaacc
      601 caatacagct acgtttggat aatgtctccg caccagctta gtcgccacta gaccgtccat
      661 tttcggcata ttgatatcca tcaccacaac ctgagggcta agctttccaa tagaagcgat
      721 agcctctgca ccattatcgg cttcacctac gacatcgata ctttgaacgc ttttcagcaa
      781 actacgaatg gcttctcgca ccatgcggtg atcatccacg agtaagactc ggattccacc
      841 cgcctgtgtt gccatgagcg gccccctttc gatacataag tatatctagc tagtaaccct
      901 agggcatcca actgtagcga agtcatgtgt ccagatcgcc taggcaaacc cctagcgact
      961 atctccccgc ctttctgaaa gcctccgttc attaactgcc tttctatttg cgcggttaag
     1021 ataagatagg caggggtact gacagaaatt gtcattccat cggtgtatac cttcaaacct
     1081 gatttaggat ttattgaccc taaccctagg gtaattgcca tcaagcaggg atggttcaaa
     1141 cggtagactt ggttgtcaaa catatgtggc caggagaggg aatcatgaac attcattttg
     1201 tcaggtttgt tgtgatagct ggtgtcattg tcgcgatagg cggttgcgcc gtctcgtcag
     1261 accgtccttc aggcgaggag gcgcaaaaag cgagtccaac tgcgcgcccc cttgatccac
     1321 agcaggccga acgtctcaaa accgtcatga taccgctcct tgccaagatg gataagccac
     1381 ttgatccctc ccaggcgtct gtcagctttt tggatagtcc tgatatcaat gccgcgaatg
     1441 ccggcggagg gaagtttttc gtcacacggg ggctgctaga aaaagcgaat gatgaacaac
     1501 ttcggggcgt catggctcat gaaatcgctc atgaggacaa aggtcatgtg actgaattac
     1561 agacgatcgg cgctggtgtt ggaatcgcct ccatgctgct aaatcagatc aatccgattg
     1621 ctggcgccat tgtgcctgta gcgggagatt tgctggttgc gaagccacac gggcgccaag
     1681 ccgagtacga ggccgatgca cacgcagtca ccattcttcg tcgatccggc cttgatggaa
     1741 aagcgatcat gacgaatacg ctcacctggc tcaagcaaac ggcgggatcc agcaaaggcg
     1801 gctttttctc tactcatctc ggtagcgacg atcggattca gaaaatccaa gagatcgcga
     1861 tgggtgtcgt tcgaacgccg ccaatcccct aatccgtcca gctgaaaggc cctggattca
     1921 gttgacaccc tgccgcaggg gtggcctcag cagagcaacc cacccttacc ctacgcgatc
     1981 agcaaggtca atctagtaac gacgccggaa ataaaagcag gtatagcact ctatgcactg
     2041 ccatggatac catccaagcc agctcaaggc atgatccatc cacccgcagc gcgtcacccg
     2101 ttggcacctg cactgacaga gagggttgga tttgagagca gcggtggaaa aacgcgaaag
     2161 ccgtacgtgg acgtccgtca gggcacgccc aatgaaggcg gtgagatgag tagttgaagt
     2221 aactagaaag tcaccggtca gtttcatgat cgggtacata ctgctctcaa agaaatactt
     2281 ggaattgggg agcctacgtg cgtcgtggca tccctttccc tcttgctcta atcccaccat
     2341 tgttccacgt cgccggtgtt cgttatttcg ttgcagaagt ccactaacgc tctcactccc
     2401 agctcgaatc tcaatcgatc tccatctaca aacgctccat tcaagaccca caggcaatcc
     2461 tgaaaaaatg gctgctgctc taccagcgtg ggggcacgat ctattctagc catcgctagg
     2521 tgccatcctt gttcccattc atagaaagtc tctttggctc gccgtctcac gataatgcgt
     2581 cttttcctca tcattgattc cccattggtt atggcttcgt cgtgtccagg tgtacggtcg
     2641 agcgcatcag gtccagacga cttgttctcg attcaggagg tactcaagca cctccatggt
     2701 gtcgcgcatt tgagttcgag cacgctctga cataggaagg acagctccac aaaccttgcc
     2761 aggctcaata tcgtggagtg aggggatgcc tccttgcctg cgtcgttcgc cttcttcttt
     2821 gtgacttgcc atatttcgat ctcccttgtt aggccgtccg atatagaagc atatgactgg
     2881 aacgcgtcct agtcacgtat ttcccgctta atgacgttac cttctgactc tttctggttt
     2941 tccgcgaagc gaagtctctg cctttacagg cggttgaata gccgcattcg atgcttcaag
     3001 agatcagtgc gaactgaaaa tgctgctatt gttgtatcgc tttgcagcgc tatcggaatg
     3061 cactttgcac aggaaggagt aatcgcacac acgcgtagcg gagggaagaa acatggagta
     3121 cctcatggca gtgtcggccg ccgtaagtgt atccggagcg ctacttttca tcacgtacgt
     3181 ttggcgagat tagcgtgcat caattccggc ttgctgctgg atcagaatga atacagatgg
     3241 tccgaaagtg atccatatgc gaatgcccgt cactctctat gaggtgcggt atggcactcc
     3301 tagcaacagt gaagcgactc ttggtggtcg aagacgatcc agatcaatct cgggctgtcc
     3361 gtgagcgttt ggaactgtac ggatacgccg tgtcgtgtgc acaggatggc cagaacgcta
     3421 tggacatgtt acaggcagag gccgctttcg acggtgtgct cttggatttg aacctgccac
     3481 atgttcccgg ccttcaggtc cttgccgcca cacgccaagc cctgcctcat gtacctgtgc
     3541 ttgtgatgtc tgcctcctcg tcccgtcttc aagccgcgaa ggaatccaat ctggcgtgcg
     3601 gatatatcgc aaagccattt ggagccgatc aattcaagca ggcggtgcga gggtgtttcg
     3661 gtcccgcata ccagccgact gccttgtagc ggaagcggat ccaacaagga gcctagcagc
     3721 atggaagatg acacaaagga aacccatgat gaagagacgt ttcggttgct gtttgaaagc
     3781 atccaggact atgccatttt cgttatgaat cctgaaggaa tcgtcaaaac ttggaatccc
     3841 ggagccgaga tgatgaaagg atatcgatcg gatgaaatcg tgggacagtc cttctctcgt
     3901 ttctatttgc cgtctgacat cgaagcggga aagccacaca tgctgttatc ccacgccgca
     3961 cgagccggtc gagtcaccga tgaaggctgg cgcgtccgaa aagatgggtc ccacttttgg
     4021 gcttctatag tcattacggc gctgtacgat caaaagggag caataaaggg gtatgccaaa
     4081 atcacacagg acatgaccga gcgaaaacaa atggaggatg acttacaaga cactctgttt
     4141 ctcctgggac aggccaataa aaaactggaa gaactggatc aactcaaatc cctctctatt
     4201 tcggtcgcga cacacgaatt gcgctctccg cttaccgccg tcaaggcgta cgttgacaac
     4261 ctattcgagg gagtagcggg cgaattgccc gagaaggtct tctactacct cacccgcatc
     4321 ggctacaaca cggaccgcgt catccggttg gtcaatatgt tgttggacat ctcgcgcata
     4381 gaggcggggc atatgcattt agaactcgat agagtgtcag tgtctgaggt catccgcgac
     4441 gtggtaaagg attttgagtg catggcgagg actaaagaga tcgccattcg cgcctcggcc
     4501 gtaatggacg cgcccgtgag agctgatcgc gacagagttg agcaagtgtt gcataacctc
     4561 ctccacaatg cgctcaagtt cactcctcca ggcggcgaga ttgtcgtgca gtcgcaactg
     4621 accg
//
LOCUS       DASTQY010000291         4785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_473285, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000291 DASTQY010000000
VERSION     DASTQY010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473285"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            467..1615
                     /gene="metK"
                     /locus_tag="VFR82_09615"
     CDS             467..1615
                     /gene="metK"
                     /locus_tag="VFR82_09615"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU4501897.1"
                     /translation="MRNNYLFTSESVTEGHPDKIADQISDGILDAMIAQDKYSRVACE
                     TILTTGIAFVAGEISTKAYVEIPDIIRDVIKDVGYCDASWGFDCNTCSVLTSIHQQSG
                     DIAMGVDSGGAGDQGLMFGYATNETAELMPMPIVLAHRLTRRLAEVRKKGILPWVRPD
                     GKSQVTVEYRNGRPIRIDTIVVSTQHSPDITSKQIERSIMEKVIKPVMPKGLYDPTSV
                     KHHINPTGRFVVGGPMGDTGLTGRKIIVDTYGGHGSHGGGAFSGKDPTKVDRSASYMA
                     RYIAKNIVGAGLADKCEVQLAYAIGVADPVSVLVDTKNTEKVSVDNLDKLVRKHFPMT
                     PRGIIEHLKLRRPIFKKTAAYGHFGRNEPEFTWEKTDKAKVLRKDAGL"
     gene            1744..2991
                     /gene="ahcY"
                     /locus_tag="VFR82_09620"
     CDS             1744..2991
                     /gene="ahcY"
                     /locus_tag="VFR82_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEU4501898.1"
                     /translation="MRDIKLADQGRLKIEWAEATMPVLRLIRKRFKRQQPLKGVRVTA
                     CLHVTTETANLAITLKAGGADVRLCASNPLSTQDDVAAALVQHEGIPTFAIKGEDNAT
                     YYRHIESAIAHRPHVSMDDGADVVSHLHSKRKDLLKNVIGGTEETTTGVIRLRSMAEK
                     KVLRFPVISVNDADTKHMFDNRYGTGQSTMDGIVRATNRLVCGSVVVVVGYGWCGRGI
                     AMRAKGMGADVIVTEIDPLKGLEAVMDGFRVMPMELAAPIGDFFVTVTGNIHVIRSEH
                     FAVMKDGAIVCNSGHFNVELDIPSLEKQAKKRRLVRPGVEEFTLRKNGRRVSLLGEGR
                     LVNLATAEGHPSSVMDMSFANQALGAEYIVKNYKHLEKKVYPVPPTIDKEIARLKLAG
                     MGVAIDTLTSEQKKYLASWEMGT"
     gene            4247..>4785
                     /locus_tag="VFR82_09625"
     CDS             4247..>4785
                     /locus_tag="VFR82_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU4501899.1"
                     /translation="MLLLVLFMPLLNAYATEPAPVDPRKMTFDAVGYLPSEPDRLVFR
                     NGLVVYLLEDHELPLITIGALLRTGGWLDPPDKVGLAALTGTVMRTGGSGGLSAAEVE
                     DELAQFAGRMSIAIGRHSGSATLDVLKKDLKRGLQLFAGALRTPAFDPAHVELAKLQA
                     IERIRRREDEPESIASREFV"
BASE COUNT         1113 a         1395 c         1344 g          933 t
ORIGIN      
        1 attcgaccag ttcgcgcgaa aattcttccc atcccacccc gggggcggga gtttgggggg
       61 tatcctgctc agacaaagtt tccggccggc tgttcatact ggtgatggtc cgtcttccat
      121 agccagacac acgatctcta cggcccgaga accacggcag attgggcggc gaggggcaat
      181 cctgcgctgg taggcgatcc ttatcccgac aatgcgtgta ccttgccgta gatcagggag
      241 gaattcctcg aaagcacagc atcttacaag cgccggaaaa ggactgtcaa gcaagcgcgt
      301 cgatggccgt ttggtcgtac cccactatcg aactaataga caaatcaagc gattatcgct
      361 atgatctgcc ccgacttcca catcaaggct tccgtctcac gcagggccgt tacccccgac
      421 tgagatcgag caaccatcgt ccgctgaaca tttttgagga gtggcaatga gaaacaatta
      481 tctctttacg tccgaatcgg tgaccgaagg gcatccggat aaaatcgccg accagatttc
      541 cgatggaatc ctggatgcca tgatcgcaca agataaatac agccgcgtgg cctgcgagac
      601 catattgacc accgggatcg cgttcgtcgc cggtgaaatt tcaaccaaag cttacgtcga
      661 aattccggac attatcagag atgtcatcaa ggatgtcggc tattgcgacg cctcctgggg
      721 gttcgattgc aatacctgct ccgtcctcac ctccatccat caacaatccg gtgatatcgc
      781 catgggagta gattcgggcg gggcaggtga ccagggactg atgttcggtt acgccaccaa
      841 cgaaacagca gagctcatgc cgatgccgat cgtgctcgcg catcgactca cccgccggct
      901 ggctgaagtg cggaagaagg gcattcttcc ctgggtccgt cccgacggga aatctcaggt
      961 caccgtcgag taccggaacg ggaggccgat ccgcatcgat accatcgtcg tctcgaccca
     1021 gcacagcccc gacatcacga gtaagcaaat tgagcgcagc atcatggaga aggtcatcaa
     1081 gccggttatg cccaaagggc tgtacgatcc gacgagcgtg aagcaccaca tcaatccgac
     1141 gggccgtttt gtagttggcg gtccgatggg tgatacgggc ctcacgggtc gcaagatcat
     1201 cgtcgatacc tacggtggcc acggcagcca cggcggtggc gcattctccg gcaaggatcc
     1261 caccaaggtg gaccgttctg cgtcgtacat ggcccggtac attgcgaaaa atatcgtggg
     1321 cgccggcctc gcggacaagt gtgaagtgca actggcctac gcgattgggg tggccgatcc
     1381 agtctccgta ctcgtcgata ccaagaatac cgagaaagta tcggtcgaca acctggataa
     1441 acttgtgcgc aagcacttcc cgatgacgcc gcgcggaatc attgagcatc tcaaactccg
     1501 ccggccgatt ttcaaaaaga ccgccgccta cggccatttc ggccgcaatg aaccggaatt
     1561 cacctgggag aagaccgaca aagcgaaggt cttgcgaaag gacgcgggac tctgatcgca
     1621 ttcggcaacg gttgatccga agatcaaggg gacggatcga gcccgtcccc tttttttctg
     1681 gcaacttgac catccattac ccgcactcat cactgcaacc ggaggttttt tgtggattac
     1741 gatgtgagag acatcaagct ggccgaccaa ggaagactca agatcgaatg ggccgaagcg
     1801 accatgccgg tcctgcggct cattcgaaaa cggttcaaac ggcaacagcc cctcaagggc
     1861 gttcgcgtca cggcctgctt gcacgtgacg acggaaaccg ccaacctggc catcacgttg
     1921 aaagccggcg gggctgacgt ccgactgtgc gcttcaaatc cgctcagtac ccaggatgac
     1981 gtggctgccg ccctcgtcca gcatgaaggc atcccgactt ttgccatcaa gggcgaggac
     2041 aatgccacct actatcgtca catcgagtcg gcgattgccc accgcccgca tgtgtccatg
     2101 gacgacggcg ccgacgtcgt ctcgcacctc cactccaagc gaaaagatct gctgaagaac
     2161 gtgatcgggg gcacggagga aaccaccaca ggtgtgatcc ggcttcggag catggcggaa
     2221 aagaaagtgc tcagattccc cgtcatctcg gtcaatgatg ccgataccaa gcacatgttc
     2281 gacaaccgct acggcaccgg ccagtcgacg atggacggga ttgtgcgggc gaccaatcgt
     2341 ctcgtgtgcg gctcggtagt tgtcgtcgta gggtacggct ggtgcggacg cgggatcgcc
     2401 atgcgcgcga agggcatggg ggctgatgtc atcgtcacgg aaatcgaccc gttgaagggc
     2461 cttgaagccg tcatggacgg cttccgggtg atgcccatgg agttggccgc tccgatcggc
     2521 gatttcttcg tgactgtcac aggcaacatt catgtcatta gaagcgaaca cttcgcagtc
     2581 atgaaagacg gcgccattgt ctgcaacagc gggcatttca acgtggaact cgacattccg
     2641 agccttgaaa aacaggcgaa aaagcgccgg ctggtcaggc ctggcgtaga agaatttact
     2701 ctccgtaaaa atggtcgccg agtcagcctg ctgggcgaag ggcggctggt taatttggct
     2761 acagccgaag gtcacccctc tagcgtgatg gacatgagct tcgccaacca ggcacttggc
     2821 gcggaataca tcgtcaagaa ctacaagcac ctcgagaaaa aggtctatcc ggttcctccg
     2881 acgatcgaca aagaaattgc gcggctgaag ttggccggca tgggcgtggc gatcgatacg
     2941 ttaacgtctg agcaaaagaa atatttggcg agttgggaga tggggacgta ggggatggcc
     3001 cggcctgcct ggctcaggca ctcggctcga ccgtgctaag cctcaggctg ctcagcgtcg
     3061 caaacttgtt cagatcacgc tttgctcgac ctgaacatca aacatgcgcc gcagtcctca
     3121 tgcttcggac cgcagccttt cggcaggcac ggcagcccga acctcacgca agtcgccagg
     3181 gcagaccaga tccatcccga cctcgccgac gaagggaagg cgaaagaagg ctggggttcc
     3241 tcggcagtca cacctccact gcgaaagaga gaaatcgagc ggtgacccag cgccgctctg
     3301 aggcttgctt caaaaacgtc cacctcgctc cacgacagag tgggaaaagc cgggcggtgc
     3361 tcgcttgggt aaacgcgcac gcctagacgt gcgcagggtt ggggcgggtg agaaagtggc
     3421 gggcacttga gagcgacccg catcacactc cgaaaaagtg gaggcaagtc agaaagcaac
     3481 cgcaggtcgc cagacagcca gaactgattc ccacactctg agcatgagaa agcgagagtg
     3541 gtaacacatc gccgctctga ttcccccttc aggaatactt cccgcctcgc tctaataaga
     3601 cgagaaaact tacccgcgta acggatcatc acgcacatag tcaggcaaac ggcaccctca
     3661 cgcctgagcc tctttatttc tatcagtcag cgtgaacggc aacccagcac cgccgatcct
     3721 caccaacgcc tgatgcaaga ccgcaactct tgccggcaca tgcaatcaaa atttgtcgtt
     3781 tcgcaacatt tttgttcatg agccagcagc ccatgtcctc gaatgggctg attaaggccg
     3841 gattttctgc agattgagct cagctgggtt caggcctaaa gtttgctcgt gacacacgac
     3901 agacgtgaag tgttcgcgaa gtctgcgtcg ttcgtctcga gaaacgcgca tgcacaacct
     3961 cttacgaacc tgttggcgct gctccggtct tactcacttt gcaaacaatc gtatgacgtc
     4021 aatgcaatag ctggcctctc ccacctgatg gggcaccatg gcggtacgga ttaggaaatc
     4081 gccgatcgga tcccacgaga ctgaacaccg agcattcgca tcccctctct ccctgacatc
     4141 ggatcgcacc acgacacgag gcgccctcca gccacgcttc gcaaccttta cgcgacggta
     4201 tcctcaaaca aaggcggcgg ctcaaggaaa cctgatacga gctggcgtgc tgctgcttgt
     4261 cctattcatg cctctcctca acgcctacgc cacagagcca gcaccggttg atccacggaa
     4321 gatgacgttc gatgcggttg gatatcttcc ctcggaacct gaccgcctgg tcttcagaaa
     4381 cgggctggtg gtctaccttc tcgaggacca tgaactaccg ctgatcacga ttggtgcatt
     4441 gttgcggacc ggcggttggc ttgatccccc ggacaaggtc ggcctcgccg cattaaccgg
     4501 aaccgttatg cggactggag ggagcggtgg cttgtctgcg gcagaagtcg aagacgagtt
     4561 ggcacagttc gcgggtcgta tgagcatcgc tatcgggcgg cactccggat cggcaacgct
     4621 tgatgtgctc aagaaagacc tgaagcgtgg cttacaactt tttgctggtg cacttcggac
     4681 accggccttc gatcctgcgc atgtggaact cgccaaacta caagcgatcg agcggatacg
     4741 cagacgtgag gacgagcctg agtccatcgc gagtcgtgaa ttcgt
//
LOCUS       DASTQY010000292        15484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_9414, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000292 DASTQY010000000
VERSION     DASTQY010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 15484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15484
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9414"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VFR82_09630"
     CDS             complement(<1..812)
                     /locus_tag="VFR82_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4501900.1"
                     /translation="MLAVDWLKRMERRTILAGGFFALTLLALVCIPRHLPSSASAPPL
                     VPANFHAHLEHGTLLLRGSLPDAGAREGILRQARAAYDPKQVRIVDQLTVDSRIASPS
                     WVGQLPAILPVLEPMQGARSVMIDGRFVVLSGAVPSEQTKAKILSTVSPLRGLGLELE
                     DHMTIAGPAASASLQARVDELLSRSQIEFDSNQATLTARGQAVLDRLIPILKEAPRTP
                     IEIGGHTDGFGAADYNKELSRRRADAVRQYLMAHGVAHDLTAVGYGASKPRAA"
     gene            962..2515
                     /gene="purH"
                     /locus_tag="VFR82_09635"
     CDS             962..2515
                     /gene="purH"
                     /locus_tag="VFR82_09635"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU4501901.1"
                     /translation="MASLKRALISVSDKTGVLDMAQGLSALGAEILSTGGTAKALRDA
                     GIMVTDVATYTGSPEILDGRVKTLHPKIHGGLLGRRSIPAHVEQMTQHNIGPIDVVVV
                     NLYPFEATIAKPNCHFDEAIENIDIGGPSMLRSAAKNHEVVLVIVDPADYQRVLEALK
                     DHTVTRALRRELAMKVFQHTARYDSLIAGYMEKQGQSGEAKFPTILSLQYELGEILRY
                     GENPHQQGAFYRERQSTEPSVSRGKILHGKAMSYNNYLDANAALELAKEYDETAVAII
                     KHNNPCGVALGATSVEAYVKARETDPTSAFGGVIAFNRPVDLPAAKEITSTFVEVVVA
                     PGFSEEALAELKRKKDLRLLDVGALTKVKQEGYDLKKLVGGLIVQDRDLGTLPDLRML
                     SVPTVRKPTDEEYAACAFAWKVCKHVKSNAIIYAKPGQTVGIGAGQMSRVDSVKLAAM
                     KAQMPVKGCVMASDAFFPFRDGLDAAAQAGITAVIQPGGSIRDAEVVKAADEHGLAMI
                     LTGMRHFRH"
     gene            2771..4042
                     /gene="purD"
                     /locus_tag="VFR82_09640"
     CDS             2771..4042
                     /gene="purD"
                     /locus_tag="VFR82_09640"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU4501902.1"
                     /translation="MKILVVGNGGREHALVWKLVQSPRQPQVFCAPGNAGIETLATCV
                     PVNASDLAALKTFVQREGIDLTVVGPEAPLALGIVDEFRRAQLKIFGPTKHAARLEAS
                     KIFSKAIMAQAHIRTARSKGFDKPADALAYLEDHELPVVIKADGLAQGKGVLIAPTRE
                     EARCAIVDSMERAVFGEAGKHVLIEQFLEGEELTIMAFTDGRSVAPMLPAQDHKRVGD
                     ADTGLNTGGMGAYCPAPLGAPALRAQVTKEILQPIVDAMSRLGSPFQGVLYAGLMVVD
                     GVPYVLEFNARMGDPETQVVLPLLKTDLLDVMEAVVEHRLDQLRVEWHDEHAVCVVLT
                     SAGYPGPYQSGARITGLDAPIEDAAMVFHAGTAKRDNDIVTAGGRVLGVTGRGRTLAE
                     ARALAYRTAERIAFAGCHYRRDIAHRALVTP"
     gene            4130..4780
                     /locus_tag="VFR82_09645"
     CDS             4130..4780
                     /locus_tag="VFR82_09645"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEU4501903.1"
                     /translation="MAPIESYSPATVERLSRQVALVIQRSGLVAIPTETFYGLGVNPF
                     DARAVDRLRAVKGREDAKPILVLVGSVNDLSSFVEHVPPVASILIEVFWPGPLTILFP
                     ARASLPLALTAGTGLVGVRLSSCQPLCELLTRVGPLTGTSANRAGAAPARTAEAVQQA
                     LGDAVDLILDAGPTPGGLPSTVVHAHDSLRIVREGVIARSVIEARLQQRGFSLKSS"
     gene            4826..5251
                     /locus_tag="VFR82_09650"
     CDS             4826..5251
                     /locus_tag="VFR82_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501904.1"
                     /translation="MPIRDMCRQRRAAGLVMAAAVLLAGCDYWPPALQTQIEQLRSEL
                     QTVTMEKAQLQNQMTSLTKAKDDLQMQVDDLSRTNRDKSTLIASLQSSVSALQERLAK
                     SSKPVAAKAPIAKVTPKPAVKTAQKAPVKKKTLTKSAVH"
     gene            complement(5270..5632)
                     /locus_tag="VFR82_09655"
     CDS             complement(5270..5632)
                     /locus_tag="VFR82_09655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEU4501905.1"
                     /translation="MPIYEYQCDGCSYQFEVKQSIKDAPLTTCERCGKSLRRLISSPG
                     IMFKGSGWYVTDYSNKLKPPSEGEKPATGEKKETPANSPSPASTTAAPAGNSATPAST
                     SSTSSPASTPPTTNTPKS"
     gene            complement(5692..6885)
                     /locus_tag="VFR82_09660"
     CDS             complement(5692..6885)
                     /locus_tag="VFR82_09660"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU4501906.1"
                     /translation="MKLAARVSRIAPSPTLAMAATAKAMAAQGIDVIDFSTGEPDFDT
                     PEPVKAAAEAAIRSGFTKYTPSSGIDELRNAIAEKLKIEQGLQYDKSQILVSCGAKHS
                     LYNLAEALLEAGDEIIIPTPYWVSYADQAFLNDATPVLLATSETDMYAIDSAALEQRI
                     TPRTKALIINSPGNPTGATYSRAMLERIAAIAVRHDLVVISDEIYEKVLYDGATHVSI
                     ASLGPEIAARTVVINGVSKAYAMTGWRIGYAAGPKDLITAMGNIQSQSTSNPCSISQK
                     AAVAALRFGGPFTKQMVTEFDRRRRAIVEGLNAIPGISCSMPAGAFYAFPNIQGVLGK
                     RGPTGPITSPTDLATYLLKSAHIAVVPGEPFGSQSHMRLSYATSMETIGRGLDRLRAA
                     LQKLG"
     gene            complement(6882..7370)
                     /gene="coaD"
                     /locus_tag="VFR82_09665"
     CDS             complement(6882..7370)
                     /gene="coaD"
                     /locus_tag="VFR82_09665"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU4501907.1"
                     /translation="MKIGIYPGTFDPITHGHTDIITRSLRVFDKVMVAVAPNPTKHPL
                     FNLAERLEMVQLVLKDIGQVEVVTFDGLLVDHVQRSGAHAIIRGLRAISDFEHEFQMA
                     LINRKLAKQVETVFLMPSEEYSYLSSTIIKDVANHGGTLTEFLHPEVARRLQARIRSA
                     KS"
     gene            complement(7367..7936)
                     /gene="rsmD"
                     /locus_tag="VFR82_09670"
     CDS             complement(7367..7936)
                     /gene="rsmD"
                     /locus_tag="VFR82_09670"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU4501908.1"
                     /translation="MRVIAGSHRGRRLSAPEGRSLRPTSDKVREALFSILGPRVSGSR
                     FLDLYAGTGAVGIEALSRGAVVVTFVESDPKVMHVMQNNLTSCGLLEQAELRREPTEM
                     FLRQRRWQHAPYEIVFADPPYADPEAVELVLAGWTIGLLAPDAVMVIEQDARAELPTA
                     TNGARFTRRYRYGDTALFLYGPAARGLPS"
     gene            complement(8507..8704)
                     /locus_tag="VFR82_09675"
     CDS             complement(8507..8704)
                     /locus_tag="VFR82_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501909.1"
                     /translation="MECPKCKGLMMLERFSDFFLIFYAWKCINCGAIIDRTISNNRRK
                     SLAARDAQTAQPAQPSVAATR"
     gene            8888..9604
                     /gene="radC"
                     /locus_tag="VFR82_09680"
     CDS             8888..9604
                     /gene="radC"
                     /locus_tag="VFR82_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU4501910.1"
                     /translation="MEQKATRQGIAYWPVTERPRERLLTQGAEALTDAQLLAILLRVG
                     RQRFSAVQVSMEILDCLGGVPGLARCGVEELCAVPGVGEAKAAQIKAAIELGKRSLAS
                     PLSKGMKIGSSRELFEHYHPTLRDLRHEIFKVVLLDAKHAIVRDATVSEGSLTLSIVH
                     PREVFTMAVKESAAAVIFLHNHPSGDPSPSQEDHVLTARLVSAGELLGIRVLDHIVVG
                     DGRYVSFADQGWLHHHAGPQ"
     gene            9686..11788
                     /gene="fusA"
                     /locus_tag="VFR82_09685"
     CDS             9686..11788
                     /gene="fusA"
                     /locus_tag="VFR82_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU4501911.1"
                     /translation="MNEPGARSLRNVAIIANRGAGKTSLAEAILLTTGAIPTLGSVTQ
                     GTTVSDFEPEEIHHRSSVSTSLLRCTWNQTTINLLDTPGALSLLGEAIAALHVADAVI
                     VVITPSSGIRSELLRLWSYVQALHLPCLIVVNELEKESASVDDVIAVCHRELEITPLP
                     MSCLWKDGAQCQGVIDFMGQQAIQSRPDSMKWQQQPIPSSAETIIGEARKRIQEAAAE
                     CDDQLLEQYLSAGELTPADVLRGLRAGVLAGTVVPLYVGSVLKNIGTVPLLNGVVDLL
                     PSPPDRAERQSLVKEHPDGHPDVSRHASPDAPFSAVIFKTIIDPFVGRLSYVRLYSGS
                     LHADSVLFNSTRRIKEKSGHLYQLLGKKHTAVSSASAGDIIAIGKLKDAQTGDTLCEE
                     AQPITHPWPTLPRPVLSFAIEPKSSADVDKVSLGLHKLIEEDPTLGFSRNADTKEMVL
                     SGTGQLHLDLALEKLQRKYGAEVVIHTPKIPYRETIRVTAQAQGKYKKQTGGHGQYGD
                     CWLEVTPSARGEGFAFTNRVVGGVIPRNFIPAVEKGVVEAMQHGTLAGFPVVDVRVAV
                     YDGSYHVVDSSEMSFKIAASMAFKKAMETAHPVLLEPMMTVEVEAPAAHIGAVIGDLN
                     ARRGRILHVEARGHAEIVKALVPLSEIMTYTTTLNSLTGGGGSYVMELARYDEVPREV
                     AAKIVEETKATKQAVGAH"
     gene            complement(11853..13244)
                     /locus_tag="VFR82_09690"
     CDS             complement(11853..13244)
                     /locus_tag="VFR82_09690"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEU4501912.1"
                     /translation="MYRRLIVLLSLLYWVAPASQAEPALAAEPAAAPAAGSNGLQLLE
                     EMQAVITELAETAKPSVVSLFPTNPLGKGRDFAPERVPNAPGSGAGVIITPDGHIITN
                     NHVVGDSAEIEVRLSDRSKMIAQVIGKDPDTDLAVLKVTADRPLPHARFGDSSTVRVG
                     QWVLAVGNPFGLERTVTLGVVSGIGRENVNLSRYENFIQTDASINPGNSGGPLFNLRG
                     EIIGINTAIINFAQGIGFAIPSNMAKQVLQQLLEQGRVVRGWLGVGIQPLTPELARKF
                     GVPEQEGVLVNEVFEKDPAAAAGIKAGDVITRIEGSVIDSPNRLSRIVAGLLPGAKAH
                     VEVVRNQERMVVEVALTERRDHATVASLPQSRSESKLGVDVQDLTAALAEKFKLRETR
                     GVLVTKVEPGSLAQSEGLREGDLIKEVNRADVGTVGDFTSAVAKVRRGDTVLLRVLRE
                     SRAFYVVLKPNEP"
     gene            complement(13316..13927)
                     /locus_tag="VFR82_09695"
     CDS             complement(13316..13927)
                     /locus_tag="VFR82_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEU4501913.1"
                     /translation="MSLEFIGRLQRELSLTGSAVYESVVAIAERVNRKVHVLRLHGQA
                     ASLLTQMETVQGELGQQLASLTPTRAGFSMTECERTLNHATDRVHRLKQTLVHVDAQI
                     RELKLEAIHGDLLTLQRDLSLRSAAIERLTVSHGCHVIGKEVCELTWPSTMRLVTVFR
                     GPFLIPPTDRFVFRADDIVIAIGSRADLDHIAPWFTVGPAGKA"
     gene            14088..>15484
                     /locus_tag="VFR82_09700"
     CDS             14088..>15484
                     /locus_tag="VFR82_09700"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248625.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HEU4501914.1"
                     /translation="MGLHPLAGQPAPESVLVDVDKLLDAYRHVPDPAVTGERVSFGTS
                     GHRGSSLKRSFNEHHILAIVQAICEYRHHQGTTGPLFLGIDTHALSRPAFDTALEVLA
                     GNGVEVMVDRHGGYTPTPVISHAILTYNRGRSSGHSDGIVITPSHNPPEDGGIKYNPP
                     QGGPADTTVTKWIEERANSILAEKLQGVTRRSGPSSKRSPLVHEHDYIGAYVADLEHV
                     IDFEPIRGSKLKLGVDPLGGSGVAYWEPIAERYRVNIEIVNASVDPTFRFMPLDWDGK
                     IRMDCSSPHAMANLIALKDRFDVAFGNDADNDRHGIVTRSAGLMNPNHYLAAAIAYLF
                     PHRPHWSAGARVGKTLVSSSLIDRVAQKLRRMLVEVPVGFKWFVDGLLDGSLGFGGEE
                     SAGASFLRRDGLVWSTDKDGIIMDLLAAEMTATTGQDPSHLYRTLTVELGEPVYARID
                     APATPQQKAALSKLSS"
BASE COUNT         3139 a         4409 c         4788 g         3148 t
ORIGIN      
        1 gcggcgcggg gtttgctggc gccgtagccg acagcggtca ggtcatgcgc gacaccatgt
       61 gccatcagat attgccgtac ggcatcggcg cgacggcggc tcaactcctt gttatagtcg
      121 gccgcgccga acccgtccgt atggccgccg atctcgatgg gcgtgcgtgg cgcctccttc
      181 aaaatgggaa tgagccgatc gagcaccgcc tgacctcggg ccgtcagggt cgcctggttg
      241 gaatcgaatt cgatctggct gcgggagagc agctcatcca ctcgggcttg gagtgaggca
      301 gaggccgccg gaccggcgat ggtcatatgg tcttcgagtt caaggccgag tccacgcagc
      361 ggcgacacgg tgctcaagat tttggccttg gtctgttcgg atggcacggc gccgctcagg
      421 acgacgaagc ggccatcgat catgaccgac cgagccccct gcatcggctc caaaacgggc
      481 aggatggccg gaagttgccc cacccaggac ggcgaggcga ttcggctatc tacggtgagc
      541 tgatcgacaa tacgcacctg cttgggatcg taggcggcac gcgcctgtcg gagaattccc
      601 tcacgggcgc ccgcatccgg gagcgaacct cgaaggagaa gcgtcccgtg ctcaaggtgg
      661 gcgtggaagt ttgccggcac cagcggggga gcagaggccg aggacgggag gtgacgaggg
      721 atacacacga gggccagcag tgtcagagcg aagaacccgc cagccagaat cgtcctgcgt
      781 tccatgcgct tcagccagtc aaccgcaagc atcacttgaa tggcaccgta tcataactga
      841 cggatgcaag ccagcccgga gtatggcccc ccgggaaagc tggtgtgttg aacttgggcc
      901 catcgtttgc tatcctccgc ccatccttcc aagccgccaa tcattctaaa ggagtgctct
      961 catggccagc ctgaagcggg cattgatcag tgtctcggat aagaccggcg tgctcgacat
     1021 ggcccagggg ctttcggccc ttggtgccga aattttgtct actggtggaa cagcgaaagc
     1081 gcttcgggat gccggcatca tggtgacgga cgtggcgacc tacaccggct cgcctgaaat
     1141 ccttgacggc cgtgtgaaga ccctccatcc gaaaattcac ggcggattgc tgggtcggcg
     1201 gtcaattccg gctcacgtgg agcagatgac gcagcacaac atcggcccga ttgatgtggt
     1261 cgtcgtcaac ctctatccat ttgaagcgac gatcgccaaa ccgaattgtc acttcgatga
     1321 ggcgattgag aacatcgata tcggcggccc ttcgatgctc cggtcggcag cgaaaaacca
     1381 tgaagtcgtg ctcgtcatcg tcgatccggc cgactaccag cgcgtgctcg aagccttgaa
     1441 ggaccacacg gtaaccaggg cgctccgccg tgaactggcc atgaaggtct ttcagcatac
     1501 ggcccgttac gacagcctga ttgctggcta catggaaaag caggggcaga gcggagaggc
     1561 gaagttcccg acaatcctgt ccctgcaata tgagttggga gaaatcctcc gctatggcga
     1621 gaatccacac cagcagggcg ccttctatcg agaacggcag tcgaccgagc cgtccgtttc
     1681 gcgcgggaaa atcctgcacg gcaaagcgat gtcgtacaac aattaccttg atgccaacgc
     1741 cgctttggag ttggcgaagg aatacgacga aactgcagta gccatcatca agcacaacaa
     1801 tccgtgcggt gtcgcgttgg gggcgacgtc cgttgaagca tacgtgaagg cgcgcgaaac
     1861 cgatccgacg tcggcgttcg gcggtgtgat tgccttcaat cgcccggtgg atcttccggc
     1921 agcgaaggaa atcacctcta ccttcgtcga ggtggtcgtg gccccgggat ttagtgaaga
     1981 ggcgctcgct gaattgaagc gaaaaaaaga tctaagactt ctggacgtcg gcgcgcttac
     2041 caaggtgaag caggaagggt acgatttgaa aaagctggtg ggcggactga tcgttcagga
     2101 tcgggatttg gggacgttgc cggacttgcg aatgctgtcg gtgcccaccg ttcgcaaacc
     2161 gacggatgag gagtatgccg cctgcgcctt tgcgtggaag gtgtgcaaac acgtcaaatc
     2221 gaacgcgatt atctatgcca agccgggtca gacggtcggc atcggagcgg gacagatgag
     2281 tcgggtggac agcgtgaagc tggctgcaat gaaggcccaa atgcccgtga aagggtgcgt
     2341 gatggcctcc gacgccttct tcccgttccg ggatggtctt gatgcggctg cgcaagccgg
     2401 gatcacagcc gtcatccaac cgggcgggtc gattcgcgac gcagaggtcg tgaaagcggc
     2461 ggatgaacat gggttggcga tgattctgac gggcatgcgt cacttccgcc attagtcggc
     2521 tgctgaaaat ggttcgtcgc gacgttctcg gtcgcctctc catccccaac gtaccgacga
     2581 atgtacgctt cggtgtcccg gcttcctgcg gccttgcgac aacccttttt gagcagcctc
     2641 accaacgatc gctggctgtg gccttgcaag ggaaagagcg cgtctcggcg cgcgagggtt
     2701 gggtgggtgg gatgagcaga tcgttcgacc atcgagatct gaccacgacg gccatcacca
     2761 aggttctcac gtgaagatcc ttgtcgttgg caacggcggc cgtgaacatg ccctcgtctg
     2821 gaagctggtg cagagtccgc ggcagccgca ggtcttttgt gcgccgggaa acgccggcat
     2881 tgagacgttg gcgacgtgtg taccggtcaa cgccagcgat ctggccgcct tgaaaacgtt
     2941 cgtgcaacgg gaaggaattg atctgaccgt cgtcggaccg gaagcgccgc tggctctggg
     3001 gatcgtcgac gaatttcgca gggcccagct caagattttc ggtcccacca agcacgcggc
     3061 ccggctcgaa gccagcaaga tcttttcaaa agcgatcatg gcccaggccc acattcgtac
     3121 ggcgcgttca aagggattcg acaagccggc cgatgcgctg gcctatctcg aggaccacga
     3181 gttgcccgtc gtcatcaagg ccgatggcct ggctcaaggc aagggggtcc tcattgcccc
     3241 cacgcgggag gaggcccgct gcgcgatcgt cgactcgatg gaacgtgcgg tctttggcga
     3301 agccgggaag cacgtgctca tcgaacagtt tctcgaaggc gaagaattga cgattatggc
     3361 cttcaccgac ggccggtccg tcgcaccgat gctgccagcg caagaccaca agcgcgttgg
     3421 ggacgcagac acggggttga atactggcgg catgggggcc tactgcccgg cgccgctcgg
     3481 cgcgccggcc cttcgcgctc aggtgacgaa agagattttg cagccgatcg tggacgcaat
     3541 gtcgcggctt ggatctcctt ttcaaggggt gttgtatgcg gggctgatgg tggtcgacgg
     3601 cgtgccgtat gtgttggagt ttaatgcccg aatgggtgat cccgaaacac aggtcgtgct
     3661 gccactcctg aaaactgacc tgctcgacgt catggaagcg gtcgtggagc accggctgga
     3721 tcagcttcgc gtcgagtggc acgatgagca tgctgtctgt gtcgtgctga cgtccgccgg
     3781 ctatcctggt ccctatcaga gtggggcgcg gattaccgga ttagatgcac ccatcgagga
     3841 cgctgccatg gtgttccacg cagggaccgc caaacgcgat aatgacattg tcacagccgg
     3901 cgggcgcgtt ctgggggtca ccggtcgagg ccgcacgtta gccgaggcgc gggcacttgc
     3961 ctatcgcaca gccgagcgaa tagccttcgc cgggtgtcat taccggcgtg acattgcgca
     4021 tcgtgcgctc gtcacacctt gactgattgg cagtggtgtc ctctggtcct ttccgattga
     4081 acaggtcact ttctgctcgg tctgaagccg gacgcactga ctcgtaaaca tggctccgat
     4141 tgagtcctat tcccctgcga cagtcgagcg tctttcgcga caggtcgctt tagtgatcca
     4201 acgcagtggt ctggtcgcga tccccacaga aaccttttac ggtcttggcg tgaatccttt
     4261 cgacgctcga gccgtggatc gcttgcgagc cgtgaaaggt cgagaagacg caaagcctat
     4321 tctcgtctta gttggctcgg tcaacgacct gtcatcgttc gtggaacacg tgccgcctgt
     4381 tgcctcaatt ctgatcgagg tgttttggcc tggcccgctg accatcctgt ttcccgcgcg
     4441 cgcctccctt ccgcttgctc tcacggccgg cacgggactg gtgggagtta gactcagttc
     4501 ctgccagccc ttgtgcgagc tgctcacgcg cgtcggccca ctgacgggca cgagtgcgaa
     4561 tcgtgcgggg gcggcaccgg cccggacggc ggaggctgtg caacaggcgt taggcgatgc
     4621 agtggatctg atcctcgacg ccggacccac gcccggtggt ttgccctcga ccgtggtgca
     4681 cgcacatgat tccctgcgca tcgtgagaga gggggtcatt gcgcgaagcg tgatagaggc
     4741 aaggcttcag cagcgaggat tttcgctcaa gagttcgtaa atgtggtata cagcaggcag
     4801 cggtgcccat ataaaggagg gatcgatgcc aatccgggac atgtgtcgac agcggcgagc
     4861 ggcagggttg gtgatggcag cggcggtgct gctggctggt tgcgattact ggccgccggc
     4921 actccagacg cagattgagc agcttcggtc tgagctccag acagtcacaa tggagaaggc
     4981 ccagttgcaa aatcagatga catcgctcac gaaggcgaag gacgatttgc aaatgcaagt
     5041 cgacgacctc agccgtacga atcgagacaa gagcactctg attgccagtc tgcagagcag
     5101 tgtcagtgca ctgcaggagc ggttggctaa atcatccaaa cccgtggccg ctaaggcgcc
     5161 cattgccaag gtgacgccga agccggccgt caagactgct caaaaagcgc cggtcaagaa
     5221 gaagacgctc acgaagtcgg ccgtgcacta gcacgaccga ccttatgcgc tagctcttcg
     5281 gcgtgttcgt cgtcgggggc gttgaggccg gtgaactggt cgaggaggtg ctggccggtg
     5341 ttgcgctgtt gccggccgga gctgctgtcg tcgaagcagg cgaaggagag ttggcaggcg
     5401 tttctttttt ctctccggtc gcaggcttct caccttctga tggcggtttc agcttgttcg
     5461 aataatcggt gacataccat ccacttccct tgaacatgat tcccggtgag gagatgagcc
     5521 ggcgaaggct tttcccgcac cgctcacagg tcgtcaacgg agcatcttta atgctctgtt
     5581 tgacttcgaa ttgatacgaa caaccgtcgc actgatattc ataaatcggc actggcggtt
     5641 cctcctccgg cggtgatacg gttcgctctt ctcacacggc cattgcagcg cttaaccgag
     5701 tttttgcagc gccgctcgca gacgatccag ccctctccca atcgtctcca tactggtggc
     5761 atacgacagt cgcatgtgtg actgactgcc gaacggttct cctggcacga cagcaatgtg
     5821 ggcgcttttc agcagatatg tcgcgaggtc cgtcggtgag gtgatcggac cggtcggtcc
     5881 ccgcttgccg agcaccccct ggatattggg gaatgcgtaa aaggccccgg ctggcatgga
     5941 acacgagatg cccggaatgg cattgagtcc ttcaacaatg gcccgccgcc ggcgatcgaa
     6001 ttcggtcacc atctgtttcg tgaatggccc gccgaaccgc agcgccgcca ccgccgcctt
     6061 ctgcgaaatg gagcagggat tagacgtact ctggctctga atgttcccca tcgcggtaat
     6121 gaggtctttg ggacctgccg catacccgat gcgccagccg gtcatcgcgt aggctttgga
     6181 gactccgttg atgacaacgg tgcgtgcggc gatctccggc cctaacgacg cgatgctgac
     6241 atgcgtcgcg ccgtcgtata aaactttttc ataaatctcg tcggagatca cgacaagatc
     6301 gtgtcggacg gcaatcgccg cgatccgctc cagcatcgca cggctgtacg tcgcaccggt
     6361 gggattgccc gggctgttga taatgagggc cttggtcctt ggcgtaatcc gttgctccag
     6421 ggcggcagaa tcgatggcat acatgtcggt ttcgctggtg gccagcaaca cgggggtggc
     6481 atcattcaag aaggcctgat ccgcatagga aacccagtag ggagtgggaa tgataatctc
     6541 atcgccggcc tcgagcaacg cctcggccaa attgtagagg gagtgcttcg cgccgcagga
     6601 caccaggatt tgcgatttat cgtattgcag gccttgctca attttgagct tctcggcgat
     6661 cgcgttgcgg agctcgtcga tgcccgatga cggcgtgtat ttggtgaagc ccgaacggat
     6721 ggcggcttcg gcggcggcct tgaccggctc cggtgtatcg aagtccggct ctccggtcga
     6781 aaaatcgatc acatcgatgc cttgcgccgc catagccttg gcggttgccg ccatggccaa
     6841 ggtcggggag ggagcaattc ggctgacgcg tgccgcaagc ttcatgactt ggcactccga
     6901 attctggctt ggagacgacg agcgacttcc ggatggagaa actcggtcag cgtcccgccg
     6961 tggttcgcca cgtccttgat gatcgtcgac gacaggtacg aatactcttc gctcggcatc
     7021 aggaataccg tttcaacttg tttggccagt ttgcggttga tgagagccat ttgaaattcg
     7081 tgctcaaaat ccgagatcgc ccgcagcccg cgaatgatgg catgggcgcc ggatcgctgc
     7141 acatggtcca ccaacaaccc gtcaaaggtg acaacttcca cctggccgat atccttcaag
     7201 accagctgga ccatctccag tcgctccgcc aagttgaaga gcggatgctt ggtcggattc
     7261 ggggccacag ccaccatcac tttatcaaat acccgcaggc tacgggtaat gatgtcggta
     7321 tggccatgag tgatgggatc aaaggtgccg ggataaatgc caattttcat gaaggcaacc
     7381 cgcgcgccgc cggcccgtaa agaaagagtg ccgtatctcc gtaccgatat cgacgtgtga
     7441 atcgagcgcc attggtggcg gttggcaact cagcccgcgc atcctgttcg atgaccatca
     7501 ccgcatcagg tgcaagaagt ccgatggtcc aaccggccaa caccagttcg accgcctcag
     7561 gatctgcata aggaggatcg gcgaagacga tctcatacgg agcgtgttgc catcgccgct
     7621 gccgaagaaa catttctgtc ggctccctgc gcaattccgc ctgctcgagc aggccacagc
     7681 tcgtcaggtt gttttgcatg acatgcatca ctttgggatc ggactcgacg aacgtgacga
     7741 caactgcgcc ccgactcaac gcttcaatgc ccaccgctcc cgtgccggca tacaaatcta
     7801 aaaagcggct gcctgatacg cgcgggccca aaattgaaaa caaggcttcc cgtaccttgt
     7861 cggatgtggg acgcagcgac cttccctctg gcgcactgag acgtcgaccg cggtgggaac
     7921 cggcgatgac tcgcatagac ttttaagacg ctactcaatc actcgcgctg actcgctcgc
     7981 gattatgacg atcatgacac tgcttgcgca ccgacgcatg ggtcggctca tgctccttgg
     8041 gcgcccagtc ccaatactca gcggcctgcg aataattcga catcaaacac tagccctggt
     8101 gtcgatcatc aaggtcgagc gagtatcgca acgacgcgca tctcggttca cctgggtctc
     8161 cggaacaggg caagtcccag gctgaccgtt gaaaaagaca ccgtccgggc tgtgtcggca
     8221 ttcttgtggg cgagaggcat tgctggcgtg tccgactgta acacagcgtt tttgcagggg
     8281 tcaaggtgac gtgaggaatg tcatcacgat cagttcacag agaagtggct agcaggccgg
     8341 cggcacaact gccacctgga ttgctggcat tttgacgatg gaagtgaggc tgactataat
     8401 tctgcaccgt gagggtgcca acgtgtgaga gggacttcat gcagccgaaa cgagacacac
     8461 tgcgggctgg ctccagcgct cgcgtccgga tgcgcgagcg agagggtcag cgtgtagcgg
     8521 cgaccgaagg ctgcgccggc tgggctgtct gtgcgtcgcg ggcggccaag cttttccggc
     8581 gattgttgga aatcgtgcga tcgataatcg cgccgcagtt aatgcacttc catgcataga
     8641 agatcagaaa gaagtcggag aaccgctcga gcatcatgag ccctttgcat ttcggacatt
     8701 ccatcgctgc ctccctggtt gcgatatgca cggctggggg cgaagcaaag caagagccga
     8761 accaattttt gtcgatacgt aatttcaaca tcttaggtgg taagtatttg ttacaagagg
     8821 ctcgtcagag gggtctctgc tagtacaaaa acgttcaacg tgtcatattt ttgtcaggag
     8881 aaatgcggtg gagcaaaagg cgacgcgaca aggcattgcc tactggcccg tgaccgaacg
     8941 tcctcgtgaa cggctgttga cgcagggcgc cgaagcctta accgatgcgc aactcctcgc
     9001 gattctcctg cgtgtcggcc gacaacgctt ttccgccgtg caggtcagca tggaaatcct
     9061 ggattgtctc ggaggagtgc cagggttggc ccgctgcggc gtcgaggagc tctgtgcggt
     9121 gcctggtgtc ggggaggcga aggcggccca aattaaagcc gcgattgaac ttggcaaacg
     9181 ctccctcgcc tcgccgctgt ccaaaggcat gaaaattggc tccagccgag agctattcga
     9241 gcactaccac ccaacattgc gagacctgcg tcatgagatt ttcaaggtgg tgctgttgga
     9301 cgcgaagcat gcgatcgttc gcgacgcgac ggtatccgaa ggcagcctga ccctcagtat
     9361 tgtgcatccg cgagaagtgt tcacgatggc ggtaaaggaa tcggctgccg ccgtgatttt
     9421 tctccacaac catccaagcg gcgatccgtc acccagccag gaggatcatg tactgacggc
     9481 gcggttggtg tcggcaggcg aactgctggg catccgcgtg ttggatcata tcgtcgtggg
     9541 cgacgggcgg tacgtcagtt ttgccgatca gggctggttg caccaccacg caggaccaca
     9601 atgagcagcg agaggggcga atgaccatgt aacctgcatg catgcggaac ggatacacgc
     9661 ttccgcacga aggaaggagg gtcctatgaa cgagcctggt gcgaggtctc tcagaaatgt
     9721 ggccatcatc gccaaccgag gggcggggaa gacgtccctg gctgaagcga tcctcttgac
     9781 taccggcgcc attccgaccc tgggctctgt gacgcagggc acgaccgtgt cggacttcga
     9841 acccgaagag atacaccacc gcagtagcgt cagcaccagc ctccttcgct gtacctggaa
     9901 tcagacgacg atcaacctct tggatacacc cggcgctctc agtttgctgg gagaagcgat
     9961 cgcggcgctg catgtggctg atgccgtcat tgtggtgatc acgccgtcca gcggcatccg
    10021 cagcgaactt cttcggctct ggagctacgt ccaagcgctc cacctgccct gtctcatcgt
    10081 cgtcaatgaa ctcgagaagg agtccgcgtc cgtcgacgac gtcatcgcgg tctgccatcg
    10141 cgagttggag atcacgcccc ttccgatgtc ctgcctgtgg aaagatggcg cgcagtgcca
    10201 gggcgtcatc gacttcatgg gacagcaggc catccagtcg cggccggata gcatgaaatg
    10261 gcagcagcaa ccgatcccct cgagcgcgga aaccatcatc ggggaagcga gaaagcggat
    10321 ccaggaggct gctgccgaat gcgatgatca attgctcgaa caatatttat ccgccggtga
    10381 gctgactccg gcggacgtac tgcgcgggct tcgggcgggc gtgctggcgg ggaccgtcgt
    10441 gccgctctac gtgggatcgg tgcttaagaa tatcggcacc gtgccgttgc tcaatggcgt
    10501 cgtcgatttg ctcccgtcac cccccgatcg cgccgaacgg cagtcgctcg tcaaagagca
    10561 tcccgatggt catcccgacg tctcgcgcca tgccagtccc gatgcaccgt tttccgccgt
    10621 catctttaag acgatcattg acccattcgt cgggcggttg tcgtacgtcc gcttgtactc
    10681 cggctcgctg cacgccgatt cggtgctctt caattccacc cgcaggatca aagagaaaag
    10741 cgggcacctg tatcagttgc ttggcaagaa acatacggcc gtgagctccg cgtcggccgg
    10801 ggacatcatc gcgattggca aactcaaaga tgcgcagacc ggcgatacgt tatgcgagga
    10861 ggcccagccg atcacccatc cttggccgac gctgcctcgg cccgtcctgt cgtttgccat
    10921 tgaacccaag tcgagtgcag atgtggataa ggtgagtctc ggattgcata aactgatcga
    10981 ggaggatccg acgttaggct tctcccgcaa tgccgatacg aaggaaatgg tgttgagcgg
    11041 caccggtcag ttgcatctgg atctggcgtt ggaaaagctt cagcggaagt acggggccga
    11101 agtcgtcatc cacacgccca aaattccgta ccgggaaacg atccgcgtga ctgcacaggc
    11161 gcaaggcaaa tataagaagc agactggcgg acacgggcag tatggggact gttggctgga
    11221 agtcacgcct tcggcacgcg gggaggggtt tgcgttcacg aaccgggtgg taggcggcgt
    11281 cattcctcgc aacttcattc cggccgtcga aaagggggtc gtggaggcga tgcagcatgg
    11341 gacgctggcc ggcttccccg tcgtggacgt ccgtgtggcc gtctatgacg ggtcgtatca
    11401 cgtcgtggat tcttcggaaa tgtccttcaa aattgcggcg tcgatggcct tcaagaaagc
    11461 gatggaaacg gcgcacccgg tgcttttgga acccatgatg acagtggagg tcgaggctcc
    11521 agcggctcac atcggggccg tgatcggcga tttgaatgct cggcgcgggc gcattttgca
    11581 tgtggaagcg cggggacatg cggaaattgt caaggcgctg gttccgctga gcgagatcat
    11641 gacctacacg acgacgctca attccttgac cggcggcgga gggagctatg tcatggaatt
    11701 ggcacgatac gatgaagtgc ctagagaggt ggcggcgaag attgtcgagg agacgaaagc
    11761 cacgaagcag gcggtggggg cgcactagcg attcgtggcg acgttggcat tgcgggcttc
    11821 gaaggcggcg tcacgacggg gcgtgcgaag gctcatggct cgttcggttt taacacgaca
    11881 taaaacgcac ggctttcacg caggacacgc aagagcacgg tgtcaccgcg ccggaccttc
    11941 gcgacggcgc tggtaaagtc gccgacggtt ccgacgtctg cacgattgac ctccttgatg
    12001 aggtcgccct cgcggaggcc ttcggattgc gcgaggctac ctggctcgac tttggtcacg
    12061 agcaccccgc gggtttcgcg aagtttgaat ttttcggcga gcgcagcggt caagtcttgc
    12121 acatcgacgc ccagtttcga ttcgctgcgg gattggggta acgaggcgac cgtggcgtga
    12181 tctcggcgtt cggtcaacgc cacttccacc accatgcgtt cttgattccg gacgacttcc
    12241 acgtgggcct tggcacctgg gagcagaccg gcaacgatcc gtgagagacg gttcggactg
    12301 tcgatcactg agccttcaat gcgggtaatc acatcaccgg ccttgatgcc tgctgcggcg
    12361 gccgggtcct tctcaaacac ttcgttgacg agcacgcctt cctgttccgg tacgccgaat
    12421 tttctggcca actccggcgt caagggttga atgccgactc cgagccagcc ccggacgaca
    12481 cgcccttgct cgagtaactg ttggaggacc tgcttggcca tgttggaggg aatggcaaag
    12541 ccgatgcctt gggcgaagtt gatgatggcg gtgttgattc cgatgatctc gccgcgaagg
    12601 ttgaagagcg gaccaccgga attgcctggg ttgatcgagg cgtcggtttg gatgaagttt
    12661 tcgtaacgcg aaagattcac gttttcccgc ccaatgccgc tgaccactcc caacgtcacg
    12721 gtgcgctcga gcccgaacgg attcccgacg gcgagcaccc actgaccaac acggacggtg
    12781 ctcgagtcgc cgaagcgcgc gtgcggcagg ggacgatcag ccgtgacctt caggaccgcg
    12841 agatccgtgt cggggtcttt accgatgacc tgcgcaatca tttttgatcg gtcggagagc
    12901 cggacttcaa tttccgcgga atctccgacg acgtgattgt tggtaataat gtggccgtcc
    12961 ggcgtgatga tgacgcccgc tccggacccg ggggcattcg gtacccgctc cggcgcaaag
    13021 tcacgtcctt tccccagggg attggtgggg aagaggctca cgacggaagg tttagctgtc
    13081 tcggccagtt ccgtgatgac ggcctgcatc tcctcgagaa gctgcaggcc atttgagcct
    13141 gcagccggtg cagcggctgg ttcggccgca agcgccggtt ctgcctgaga agccggtgca
    13201 acccaataga gcagggacaa caggacgatg agtcggcggt acatactttt agtgtacctc
    13261 aggtgactgg gaaaacctat caggtctgaa gcatggaatg gaaactcacg acgggtcagg
    13321 ctttgcccgc agggccgacg gtaaaccagg gggcgatgtg atccagatcg gcgcgggagc
    13381 cgatggcaat cacgatgtcg tcggctcgga agacgaaccg gtcggtaggc ggaatgagaa
    13441 agggaccacg aaataccgtg accaatcgca tggtcgacgg ccacgtcagc tcgcagactt
    13501 ctttgccgat cacatgacag ccgtgtgaga ctgtgaggcg ctcgatggcg gccgagcgga
    13561 gactcaggtc gcgctgcagc gtgagcagat cgccatggat cgcttccagc ttgagctccc
    13621 ggatctgggc atccacgtga acgagcgtct gctttagccg atggacacgg tcggtcgcgt
    13681 gattgagcgt gcgctcacac tcggtcatgg aaaagccagc gcgagtcggc gtgagactcg
    13741 ccagctgttg gcccagttcg ccctggacgg tttccatctg ggtcaacaag ctggctgctt
    13801 gtccgtgaag ccgcaagaca tggactttgc ggttcacacg ctcggcaatc gccacaacgg
    13861 actcgtagac ggcgctgccg gtcagcgaca gctctctttg gaggcggcca atgaattcta
    13921 gcgacatgga atggttaatg tctgataagg gatattatgt caacttgtac ttcttccctc
    13981 aatccagggc ctgcggctcc cgtacttact ctcttctccc cttccggtca atccaaccag
    14041 ctgctgtcgt agattgacaa gccgagcctg caaggctaca ccaaggcatg ggtcttcatc
    14101 ccttagccgg tcagcccgcg ccagagagcg ttcttgtcga tgtcgacaag ttgctggatg
    14161 cctatcggca cgtgcccgat ccggccgtca ccggcgaacg ggtcagtttt ggtacctcgg
    14221 ggcatcgcgg ttcatccctc aagcgatcct ttaacgagca tcacatactc gccatcgttc
    14281 aagcgatttg tgaataccgg catcaccaag ggactaccgg tcccttattc ctgggcattg
    14341 acacgcatgc gctctcgcga cccgcattcg acacggctct ggaggttttg gctggcaacg
    14401 gggtcgaagt gatggtggat cgtcatggcg gctatacgcc gaccccggtg atctcccatg
    14461 ccattcttac ttataatcgc ggccggtcat ccggacacag tgacggtatc gtgatcacgc
    14521 cgtctcacaa tccccctgaa gacggcggca tcaagtacaa ccctccacaa ggcggaccgg
    14581 ccgatacgac cgtaacgaaa tggatcgaag agcgagccaa tagcatcctg gccgaaaagc
    14641 tgcagggtgt gactcgtcga tccggaccat cgagcaagcg tagtccattg gtacatgagc
    14701 atgactatat cggggcatat gtcgcagatc tggagcacgt gatcgatttc gagccgatcc
    14761 gcggcagcaa acttaagttg ggcgttgatc cgctcggcgg ctcgggagtg gcctattggg
    14821 aacctattgc agaacgctat cgtgtgaaca tcgaaatcgt caatgcgagc gtcgatccga
    14881 cctttcggtt catgccgctg gattgggacg gaaaaatccg gatggattgc tcttctccgc
    14941 acgccatggc gaatctgatc gcgctcaaag atcgatttga cgtggcgttc ggcaatgatg
    15001 cggacaacga ccgtcatggc atcgtcacgc gctcggccgg tctcatgaat ccgaatcact
    15061 atttggccgc ggccattgcc tatctcttcc cccatcggcc gcactggagc gcgggtgctc
    15121 gcgtgggcaa gacgttggtc agcagcagct tgatcgatcg tgtcgcgcag aaactccgtc
    15181 gcatgctggt ggaagtgccg gtcggcttca agtggtttgt cgatggattg ttggatggtt
    15241 cgctcggttt cggaggggaa gaaagcgcgg gggcatcgtt cctgcggcgt gacggcctcg
    15301 tctggtctac cgacaaggac ggtatcatca tggatctgct ggcagctgaa atgacggcga
    15361 cgacgggaca ggatccttcc cacctgtatc gcacgctcac ggttgaattg ggtgagccgg
    15421 tgtatgcgcg tatcgacgca ccagccaccc ctcagcagaa agcggcgctc tcgaaacttt
    15481 cctc
//
LOCUS       DASTQY010000293         7048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_80545, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000293 DASTQY010000000
VERSION     DASTQY010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80545"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..2553)
                     /locus_tag="VFR82_09705"
     CDS             complement(145..2553)
                     /locus_tag="VFR82_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEU4501915.1"
                     /translation="MGNALSEKELKLIDAYWRAANYLSVGQIYLYDNPLLKKPLKKEH
                     VKPRLLGHWGTTPGLNFIYAHMNRVIKQRDLNMIYIIGPGHGGPGIVANAYMEGTYSE
                     VYPNISLDEEGMKRLFTQFSFPGGIPSHVAPETPGSIHEGGELGYAVSHAFGAAYDNP
                     DLIVTCVVGDGEAETGPLATSWHSNKFLNPLSDGVVLPILHLNGYKIANPCVLARISH
                     QELDQLFRGYGYTPHFVEGSDPEKMHQTMAATLDNIIEEIHHIKTEARRTEGKKRPLW
                     PMIILRSPKGWTCPKEIDGKRTEDYWRSHQVPMGEMHEKPEHIKILEKWMKSYKPEEL
                     FEKTGRLKPELAELPPKGDRRMSANPHANGGLLLKDLRLPDYRDYAVEVPKPGATTAE
                     STRLMGKFLRDTMKLNMEFKNFRLFSPDENNSNRWQDVLEVTNRTWMAESYPYDDHLA
                     PNGRVMEMLSEHQCQGWLEGYLLTGRHGFFSCYEAFIHIIDSMLNQHAKWLKVCNEIR
                     WRRPIASLNYLLSSHVWRQDHNGFSHQDPGFIDHVVNKKADVVRVYLPPDGNTLLSVT
                     DHCLRSRNYVNVIVAGKQPAPQWLTMDQAIKHCTAGIGIWEWASNDKGTDPDVVMACC
                     GDVPTLETLAAVDIIRQHVPALKIRVVNVVNLMKLQPPSEHPHGLSDFDFDALFTKDK
                     PIVFAFHGYPWLIHRLTYRRTNHKNLHVRGYKEEGTTSTPFDMVVMNDLDRFHLVSDV
                     VDRLPQLGSRAAYAKQAIKEKLLEHKQYINRYGEDMPEITNWQWGQGSEKTKRRSSTE
                     GDNV"
     gene            complement(2586..3824)
                     /locus_tag="VFR82_09710"
     CDS             complement(2586..3824)
                     /locus_tag="VFR82_09710"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU4501916.1"
                     /translation="MLVLVINSGSSSVKFRLVEVVEGENQGFTTRSPRLSGAVKSIGS
                     AATFEVTGQSPRSTTLKIRDHAEALRVVFESLSDSLEDIHAVGHRVVHGGDRYMEPTL
                     INDQVEAGIDALADLAPLHNPACLAGIRGAKAMLSSAVPMVAVFDTAFHQTMPEVAKR
                     YALPVELADRHRIRRYGFHGIAHASLAHGYAAIVGTPLKGLRLITLQLGNGCSIAAID
                     GGRSVETSMGFTPLEGLVMGTRSGDVDASIVSYLSEREKVEPGEVERWLNERSGLFGI
                     SGRSNDMKELLRASEQEHDKRAEFAIELFCYRVRKYLGAYLAVLGGADAIVFGGGIGE
                     NAPEVRRRICLGMDWCGWRLDSNRNRAAVGLPHGAGTKISTDDSKVMVYVVAADEETW
                     IAKETARCVRSGNTIAPNIN"
     gene            complement(3868..4770)
                     /locus_tag="VFR82_09715"
     CDS             complement(3868..4770)
                     /locus_tag="VFR82_09715"
                     /inference="COORDINATES: protein motif:HMM:NF003784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HEU4501917.1"
                     /translation="MKTLSAREQQQQKMRTHPGFIAALDQSGGSTPNALRLYGIKESE
                     WSNDKEMFDLVHQMRARLIISPSFSGERILAAILFENTMERDIEGRPTADYLWNVKRV
                     VPFLKVDKGLAPEKDGVQLMKPMPDLAALLDKANRKGIFGSKMRSLIKEANGAGIKNV
                     VSQQLDIARQIISAGLVPIVEPEVDIHSREKAKAEELLKEVLLKALNELPAGHLVMLK
                     LTLPERDDFYLECIRHPNVLRVVALSGGYTRDEANDRLRKNHGMVASFSRALVEGLTA
                     QQSDAEFNAMLDKYIQSIFDASAT"
     gene            5183..5767
                     /locus_tag="VFR82_09720"
     CDS             5183..5767
                     /locus_tag="VFR82_09720"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU4501918.1"
                     /translation="MTAPPQQVFIVRHGETEWSLSGQHTGTTDIPLTEHGRQVARLLR
                     PILAKESFALALTSPLQRATETCRLAGFEKAVKIEPDLVEWNYGLYEGFTPEQIHKTA
                     PGWLIFRDGCPGGEQPEEIGARVDRVIANVRAVEGNVILFAHGHVLRVFVARWLALPP
                     QAGQHFLLDTATTNILGYYRTVPAIKRWNAPVQL"
     gene            5913..6809
                     /gene="atpG"
                     /locus_tag="VFR82_09725"
     CDS             5913..6809
                     /gene="atpG"
                     /locus_tag="VFR82_09725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEU4501919.1"
                     /translation="MPSLQSVRRKIDSVKKTQKITKAMKMVAAAKLKRTQDRILAARP
                     YALKMRDTIRSLSRRVNRDAHPLLKKREERRIVVAVVTSDRGLCGAFNANILRTAAAA
                     LKEFESRGMQIEVAVIGRKGRDFFRRRSWKVRREVVDIFDKLSFEHGMLLGSELQAID
                     DYVTERIDAVYAIYNEFKSAIQQRVVVERLLPIDWVVEIADVKEVERTTEQMPGGYLY
                     EPSEDEVLQELLHRNFHVQAYRMLLESSAAEQGARMTAMDGATRNAGELIKKLTIYYN
                     KTRQAAITKELMDIVGGAEALK"
     gene            complement(6839..>7048)
                     /locus_tag="VFR82_09730"
     CDS             complement(6839..>7048)
                     /locus_tag="VFR82_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501920.1"
                     /translation="WWTVLQTIWQVDFERSGKSSMTMEFEAGRSLVGRFGAYVRPGVG
                     IWGRDVAGAYQWNIEVGVRYMFPSF"
BASE COUNT         1483 a         2081 c         1885 g         1599 t
ORIGIN      
        1 tgccgttcgt acagagcatc gctggtgccg gaaaattgta tgggtcccac cccatattgt
       61 tggagaatgt ctttgaggcg ttcctgtttc atacaagcct cttcgcaaag gttagaggaa
      121 gcgtggggcg tatgctgcaa gccactagac gttgtctcct tccgtcgaac tccggcgctt
      181 ggtcttctca ctcccctggc cccattgcca attggtgatc tccggcatat cctccccata
      241 ccgattgatg tattgcttgt gttcgagcag cttctccttg atcgcctgtt tggcataggc
      301 ggcccgtgac cccagctgcg gcaagcggtc cactacatcg ctgacgagat gaaagcggtc
      361 gaggtcgttc atgacgacca tgtcgaaggg cgtgctcgtc gtgccttcct ctttataccc
      421 ccggacatgc aggttcttgt gattggtcct tcgataggtg aggcggtgga taagccaggg
      481 atagccgtgg aacgcgaaga caatgggctt gtccttcgta aagagtgcat cgaaatcgaa
      541 gtccgacaga ccatgcggat gctcgctggg cggctgcaat ttcatgagat tcacgacgtt
      601 gacgaccctg atcttcaatg ccgggacgtg ctgtcgaata atgtccaccg ctgctaacgt
      661 ttcgagcgtg ggcacgtctc cgcagcaggc catcacgacg tctggatctg ttcccttgtc
      721 attgctcgcc cattcccaga taccgatgcc ggccgtgcaa tgcttgatcg cctgatccat
      781 cgtcaaccac tggggtgccg gttgcttgcc ggccacgatg acgttcacat agttccggct
      841 tcggagacag tgatcagtca cagacaacag tgtattgcca tcgggcggaa gatagacacg
      901 caccacgtcg gctttcttgt tcacgacgtg atcgataaac cctgggtcct ggtggctaaa
      961 cccgttgtgg tcctgtcgcc acacgtggga gctcaataaa taattgagcg aggcaatcgg
     1021 ccttctccaa cgaatctcat tgcacacttt cagccacttc gcatgttgat tcagcatcga
     1081 gtcgatgatg tgaatgaacg cctcgtaaca ggaaaaaaac ccgtgccggc cggtcaggag
     1141 gtaaccttcc agccaccctt ggcactggtg ttcgctcaac atttccatca cccggccatt
     1201 cggcgccagg tgatcgtcgt acggatagct ctcggccatc catgtccggt tggtcacctc
     1261 caagacatcc tgccatcggt ttgaattgtt ctcgtccgga ctgaacagac gaaagttttt
     1321 gaactccatg ttcagtttca tcgtatcgcg caggaatttg cccatgagcc gggtagattc
     1381 tgcggtggtg gcgcccggtt ttggcacttc gacggcatag tcgcgatagt caggcaaacg
     1441 caaatctttc aggagcaacc cgccattggc gtggggattg gcactcatgc ggcgatcgcc
     1501 cttcggcggc agttcggcca gctccggctt gaggcggccc gtcttctcga agagctcttc
     1561 cggcttgtaa ctcttcatcc atttctcgag gatcttaatg tgctcaggct tttcgtgcat
     1621 ttcacccatc ggcacttggt ggcttcgcca atagtcttcg gtccgtttcc cgtcgatttc
     1681 ctttgggcac gtccatcctt tcggtgagcg gaggatgatc atcggccaca gcggtcgttt
     1741 cttgccttcc gtccgtcttg cctcggtctt aatgtgatga atttcttcga tgatgttgtc
     1801 cagcgtggcc gccatggttt gatgcatctt ctccggatcg ctgccctcga caaagtgcgg
     1861 cgtatagcca tagccgcgga agagctgatc cagttcctga tgactgatgc gggccaagac
     1921 gcagggattg gcgatcttgt aaccgttgag gtgcaggatc gggagcacga cgccgtctga
     1981 aagcggattg agaaacttat tcgagtgcca ggaggtggcc agcgggccgg tttctgcctc
     2041 gccgtcccct acgacgcaag tgacgatcag atcgggattg tcatacgccg ctccaaacgc
     2101 atgcgacacc gcgtagccca attcgcctcc ttcatgtatg gaaccgggcg tctctggcgc
     2161 gacgtgactg ggaatgccgc cgggaaaaga aaactgagta aacaagcgct tcattccctc
     2221 ttcatccagg ctgatgttcg gatagacctc cgagtaggtc ccttccatat aggcattggc
     2281 gacaatgccg ggcccaccgt ggccagggcc gatgatgtaa atcatgttga ggtcccgctg
     2341 cttgatcacc cggttcatat gcgcgtagat gaaattcagg cccggtgtcg ttccccaatg
     2401 accgagcagg cgcggtttga cgtgttcttt tttcagcggc tttttcaaca gcggattgtc
     2461 atacaagtag atttggccga cggacaggta attcgccgcg cgccagtagg cgtcgatcag
     2521 cttcagctct ttctccgaca atgcatttcc catgttggac tccttccctg tgttgcagtc
     2581 ttccctcaat tgatgttcgg cgcgatcgtg ttaccggatc gcacgcagcg agccgtctct
     2641 tttgcaatcc acgtttcttc gtccgctgcc accacataga ccatcacctt cgagtcgtcc
     2701 gtgctgatct tcgttcctgc cccatgcggg agcccgactg cggctctatt gcgattggaa
     2761 tcaagcctcc acccgcacca gtccatgccc aggcagattc tccttctcac ctccggggcg
     2821 ttttctccga tcccacctcc aaacacgatc gcatccgcgc cgcccaagac ggcgaggtag
     2881 gcgccaagat acttcctcac gcgatagcaa aagagttcga tggcgaactc ggcccgcttg
     2941 tcgtgttcct gttctgacgc gcgcagcagc tctttcatat cgttcgatcg acccgatatc
     3001 ccaaacagtc ccgaccgctc attgagccac cgctcgactt ctcccggctc gaccttttcc
     3061 cgctcggaga ggtagctcac aatggaggca tccacatcac cagacctggt ccccatcacc
     3121 aacccttcga gcggcgtgaa gcccatcgac gtctcgacgg accggcctcc gtcaatcgct
     3181 gcaatggagc agccattgcc caattgcaag gtaatgaggc gcaggccttt cagcggcgtt
     3241 ccaacgatgg cagcatagcc atgcgcaagg gaagcatgcg cgatcccatg aaatccatag
     3301 cggcggatgc gatgtcggtc ggcgagttcc acaggcaacg cataccgttt ggcaacttcc
     3361 ggcatggtct gatgaaaggc ggtatcgaac acggccacca tcggcacagc cgaactcagc
     3421 atcgctttgg ctccacgaat cccggcaaga caggcaggat tgtgcagcgg cgccaagtcg
     3481 gcgagtgcgt cgattcctgc ctcgacctgg tcgttgatca acgtgggctc catgtaccga
     3541 tcgcccccat ggacgacgcg atggccgacc gcgtgaatgt cctcgagaga atcggaaaga
     3601 gactcgaaca cgacgcgtaa cgcctcggca tggtcgcgaa tcttcaacgt cgtcgaccgc
     3661 ggggattgtc ctgtgacctc aaaagttgct gcactcccga tgcttttcac cgcaccagag
     3721 agtcgcgggg aacgagttgt gaatccttga ttctccccct cgactacttc aacgagccga
     3781 aatttaacag acgaacttcc gctattgatc acgagaacca gcatgtcaac gccgaaccgg
     3841 caatcgtgac attagccttt gtcattccta cgtagcggac gcatcaaaga tgctctggat
     3901 gtatttatcc agcatcgcgt tgaattcggc atcggattgc tgggccgtca gcccttcgac
     3961 cagcgcccgc gaaaagctcg ccaccatccc gtgattcttg cgcaaacggt cgttggcttc
     4021 gtcccgcgta tatccccccg atagcgcgac cactctcaag acattcggat gtcggatgca
     4081 ctcaaggtag aaatcatctc gctcagggag tgtgagcttg agcatgacca aatgacccgc
     4141 cggcaattcg ttgagtgcct tcaaaagaac ctctttgagt agctcttctg ccttggcctt
     4201 ttcgcgagag tgaatgtcaa cttcaggctc cacgatcggc acgaggccag ccgagataat
     4261 ttgtcgggcg atatcgagct gctggctcac gacgttcttg atgccggctc cattggcttc
     4321 tttgatgagt gagcgcatct tgcttccaaa gatgcctttc ctgttggctt tgtcgagcag
     4381 tgccgcaagg tcgggcatag gcttcatcaa ctggaccccg tccttttcag gcgcgaggcc
     4441 tttgtccact ttgagaaaag gaaccactcg cttgacgttc cacagataat cggccgtagg
     4501 ccgtccctcg atgtcccttt ccatcgtgtt ctcgaacagg atggcggcaa gaatccgttc
     4561 gccgctaaaa ctcggactga taatgagacg cgcccgcatc tgatgcacga ggtcaaacat
     4621 ctccttgtcg ttggaccact cactttcttt gatcccgtag agacgcaacg cgtttggcgt
     4681 gctgcccccg ctttgatcta gtgccgcaat gaaaccggga tgcgtcctca ttttctgttg
     4741 ttgttgttcg cgagcgctga gagttttcat aacagtttct ccagggtgag cgtggtggaa
     4801 agagccgacg gctcgactcg aagtttacgc cattgttctt taattgttct tcaaaggcac
     4861 gcccagcctt tgaaaattcc acagctgtgc cgcttgtcat ctttagtcag ctgggaaagc
     4921 actttacaac gatcattagc agagcgaaca ctgtcaaaac tgacaggctg ccctcctcct
     4981 ggatgtgctc caccgcgtat tggcggccgg acactgtacc ccattgaggg gccaggagaa
     5041 ctagtagaag cccaagaaca gcgcaggaga agaatagttc gatgcacctt cccatcttcc
     5101 atccggcatg gaagtttgct agcatcccca tcaccctaac tatccgggac gcctcaccgt
     5161 ctgcgtaaca caagcacctc aaatgactgc cccaccacaa caggtcttca ttgtgcgcca
     5221 cggagaaacc gagtggagtc tcagcggaca acacacggga accactgaca tcccgttgac
     5281 tgaacacggc cgacaggtgg ccaggctgtt gcgaccgatc ctggctaagg aatcgtttgc
     5341 gctggctctg accagcccgc tgcagcgagc gacggagact tgccgcctgg ccggttttga
     5401 gaaggcggtg aaaattgagc ccgatcttgt cgagtggaat tatgggcttt acgaggggtt
     5461 cacgccggag cagattcaca agaccgcgcc cggttggctc atttttcgag atggctgccc
     5521 cggcggcgaa cagccggaag agatcggcgc gagggtcgat cgtgtcatcg ccaacgtgcg
     5581 tgcggtcgag ggcaatgtta tcttattcgc tcacggtcac gtcctccggg tcttcgtcgc
     5641 tcgctggctc gccctccctc ctcaagcagg gcagcatttt ctgctcgata cggcgacaac
     5701 caacatcctc ggctattatc gcaccgttcc ggcgatcaag cggtggaacg caccggttca
     5761 attgtgattg ggttcacaca gcatcggccc atcggacggt cgcgctcatg atccatagcg
     5821 tgcgtttcaa gcttgacgct cccagcacac aatgatagcg ttcgtctttc acttccgact
     5881 ccggttcctg atggacagga ccaataacgg ttatgccaag tctccaatca gttagacgga
     5941 agatcgactc cgtcaagaaa acccaaaaga tcaccaaagc gatgaaaatg gtcgcggcgg
     6001 ccaagctgaa acgcacgcaa gaccggattc ttgccgcacg accgtacgca ctgaagatgc
     6061 gcgataccat tcgctccctc agccggcgtg tgaatcggga cgcccatcct ctgctcaaga
     6121 agcgtgaaga gcgccgcatc gtggtggctg tagtcaccag cgaccgcgga ttgtgcggcg
     6181 cctttaatgc caacatcctt cgtacggcgg cagccgcgct gaaagagttc gagtcaaggg
     6241 gtatgcagat tgaagtagcg gttatcggca gaaagggccg cgattttttc cgtcgacgca
     6301 gctggaaagt gcgacgcgaa gtggtagata tcttcgataa actctcgttc gaacatggga
     6361 tgctcttagg gagcgagctt caagcgattg acgactatgt gacagagcgc atcgacgccg
     6421 tctatgcgat ctacaatgag ttcaagtccg ccatccaaca gcgtgtggtc gtagaacggc
     6481 tccttccgat cgattgggtc gtcgagattg cagacgtaaa ggaggttgaa cgcaccacgg
     6541 agcagatgcc cgggggctat ctctacgaac cgagcgaaga cgaggtgttg caggaactgc
     6601 tacaccgcaa tttccacgtc caagcctacc gcatgctact ggaatcgtcg gcagccgaac
     6661 agggagcccg catgacggcc atggacggag cgacgcgcaa tgccggcgag ctcatcaaga
     6721 agctgacgat ttattacaat aagacccggc aggctgcgat caccaaggaa ctgatggata
     6781 tcgtcggcgg cgcagaagcc ctgaaataac ccctcaggcc cccaccttcc tgttcacgtc
     6841 agaagctggg aaacatgtag cgaaccccga cttcaatgtt ccattggtac gccccggcga
     6901 catcacgccc ccaaattccc acccccggtc ggacgtaggc tccaaaccgc ccgaccagac
     6961 tcctaccggc ctcgaattcc atggtcatgc tgctcttccc gcttcgctcg aaatcgacct
     7021 gccagatggt ttggagaacg gtccacca
//
LOCUS       DASTQY010000294         5062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1028850, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000294 DASTQY010000000
VERSION     DASTQY010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028850"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VFR82_09735"
     CDS             complement(<1..720)
                     /locus_tag="VFR82_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU4501921.1"
                     /translation="MPNRPSMSDTAAREAAETTYDRNVVVVAGAGTGKTTLLVNRLVH
                     LLMKEPDPVGLIQVVALTYTNKAATEMKVRLRERLAMLAQAATPGGTGPDTGTIVIED
                     LRLRYGITVEEIAARAIAALQDLEKAQIGTVHSFAAHLLRLYPLESGVDPAFREDDGV
                     RFDEHFRMAWDIWIDRELSRQGTQHKLWRALLESTTVAQLRTLTSSLCSDLIDVEALT
                     RQLASTQRPWRAGRARCRAWPS"
     gene            complement(713..3871)
                     /locus_tag="VFR82_09740"
     CDS             complement(713..3871)
                     /locus_tag="VFR82_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEU4501922.1"
                     /translation="MLRVVTGRFQPSLESALVNHIQRIKGADVWSQIAIVVPSKPLLD
                     RIRWLLAIEHQLALVNVHFLTFHQLALRLAEETRREGTGHPPRVVDELFFEYLVRHLV
                     QRRLAELPALRQLGRSFGTWAGLWSTVRDLNDGGVDPATALRAAGEGYFGQDDADWLR
                     ALFSLHAAVREVGKGFHVVTADDLTEAVLPAVPASPFLTSLSHISYYGFYDLTQVQLS
                     MFQAVSGTVPTTLFFPLATDSSFNFARRFFDRHVQPLLKSNDDLVEAADSSEAFAHVD
                     VALRSVVGQEEELALACRTILDLVETHGYRFDEIGVVARTLDPYYSAARNIFDRHRIP
                     FSTPARRPLIHEPLCKTLLQLAALPANDFYRAAVLDLVTSPFYIVPLSSGRFVRCRSY
                     RPEQWKLVLDALHITHGVDEWKRLEQASQSALEWDVEDDAAGIIGSVKVAPDVIGLLW
                     ETVSELLNSYSTLPARGTIGSLIHAFRELMARHVHRAEEESAIWTAVDQACAAVGELD
                     AIGEDITWMDLAELLTHAFERAAIPMEPTPHQGVRLLDAMTARGLPFKALFVLGLNDK
                     VFPRYVREDAFLRDRHRRVLDTTLGFKVDEKLTGYDEERLLFSLICGSADRRLYLSFQ
                     RADDAGRMLAASPYIGGELRRLGLSDVEVEMIPRRLTDRIRQRPAISRFLPPEDLAQW
                     MAVQGEDPSELLHATGRDAALFHRGVEALGLIEDDRAALTAFDGVTGPLKNHWARIAE
                     RGIAPTPLERYASCPFRYFSTDVLRLDSVRVSPSDEPDARLLGTVCHAALRRCYELLL
                     PTGWPAKPVTDDTVDWCIKTAVEQAAEEQETLHRTGHYLLWELAKSQIIDVVTAAVDD
                     DTRAHLHEPFLPIGFEVDADGNMDDLRMNAPSPLRVRGRIDRLDRHRDSGALRVIDYK
                     FKIGNSMKPQDRNLLLSAIRGYRLQPPMYARLNVPAHGRVHEVQLFFLAPNWPTPVVR
                     STFKTDLWSSDSGTLLRTTFAQLVDGLRNGSFFILPDTYCETCDYRVACRREHTLTSW
                     RASRAAEAKALTLLRTSRVNDA"
     gene            complement(4247..4513)
                     /locus_tag="VFR82_09745"
     CDS             complement(4247..4513)
                     /locus_tag="VFR82_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU4501923.1"
                     /translation="MPNITLLISPSCGACPSAKSLWKQLRVKYSFSYREVDITTPDGQ
                     ELANRHAVRAVPATIIDGRLTFVGVPSRESAEKALQLKMRPREG"
     gene            complement(4699..>5062)
                     /locus_tag="VFR82_09750"
     CDS             complement(4699..>5062)
                     /locus_tag="VFR82_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501924.1"
                     /translation="HPATLAVTLRAFLQWSEARKLSKPLGRRADKYYVKGALMTRRTI
                     ITAAGAFMVLSLVSACNRDQDQTTPVGQQQGQGSAQDYPSTRNMEQPNPAIGGTNSAT
                     NKPAPSGNQEPKAGSATR"
BASE COUNT         1076 a         1444 c         1491 g         1051 t
ORIGIN      
        1 gctcggccag gctctgcagc gtgctcgccc agcgcgccac ggtcgctgcg tcgaagcgag
       61 ctgccgcgtg agcgcttcga catcgatcag atcactgcac agagatgagg tcagtgtgcg
      121 aagctgcgct accgtcgtcg attcgagtag tgctcgccag agcttgtgtt gtgtcccctg
      181 tcggctcagc tcccgatcga tccagatgtc ccacgccatt cgaaaatgtt cgtcgaaccg
      241 caccccatcg tcctctcgga atgcagggtc cactccgctc tccaacggat acaaacgaag
      301 gagatgagcg gcaaaactgt gcacagtgcc aatctgggct ttttccaaat cctgcagggc
      361 ggctattgcc cgggccgcaa tctcctcgac cgtgatgccg taccgaagcc gcagatcttc
      421 gatgacaata gttccggtat caggcccagt gcctcccggt gtggcggctt gagccagcat
      481 cgccaagcgc tcgcgcaggc ggactttcat ctctgtggct gccttattcg tataggtcaa
      541 ggcgacgacc tgaatgagtc cgaccggatc cggctccttc atgagcagat gaaccagtcg
      601 gttcaccagc aaagttgtct ttccagttcc tgccccggcc acgacgacca cattgcggtc
      661 gtacgtcgtt tctgccgctt ctcgagcggc cgtatcagac atgctcggtc ggttaggcat
      721 cgttgactcg cgaagttcgt aacaatgtca gcgccttcgc ttcggcagcg cgtgatgcac
      781 gccacgacgt cagcgtatgc tcacgtcggc aagccacgcg ataatcacat gtctcgcaat
      841 aagtgtccgg caagatgaag aaggagccgt tgcgcaggcc gtcgaccagt tgagcgaagg
      901 tggttcggag caaggtgccg ctgtcagaag accagagatc ggtcttgaat gttgagcgta
      961 cgacgggagt tggccaattc ggcgccaaaa agaaaagctg gacttcgtga accctgccgt
     1021 gagccgggac attcaaccga gcatacatgg gcggctggag tcgatagcct ctgatcgccg
     1081 ataatagaag gttacgatct tgcggtttca ttgagttgcc gattttgaat ttgtaatcga
     1141 tgaccctcag agcaccggaa tcgcgatgcc gatcgagtcg gtcgatgcgc ccccgcacgc
     1201 gtaaaggtga aggtgcgttc atgcgcagat cgtccatgtt gccgtctgca tcaacttcga
     1261 aaccgatggg tagaaagggc tcgtgaagat gggcgcgtgt atcgtcatca acagcggctg
     1321 tcaccacatc gatgatctgg ctctttgcca gttcccaaag gagatagtgt cctgttcggt
     1381 gaagggtctc ctgttcttcg gccgcctgtt caaccgccgt cttgatgcac cagtcgacgg
     1441 tgtcgtcagt gaccggtttc gccggccatc ctgtcggaag cagcaattca taacagcgtc
     1501 gcagagctgc gtggcacacg gtgccgagca aacgggcgtc cggctcatca gaaggagata
     1561 ctcggaccga atccaatcga agcacgtcgg ttgaaaagta cctgaagggg caactcgcgt
     1621 accgttcgag cggcgttggc gcgatcccac gttcggcgat gcgcgcccag tggtttttca
     1681 atggaccagt cacaccgtcg aaggctgtca aagccgcacg atcgtcttca atcagcccga
     1741 gcgcctcgac cccacggtgg aagagagcgg cgtcgcggcc tgtggcgtga agaagctcgg
     1801 agggatcttc cccctgcacc gccatccact gagccagatc ttcagggggg aggaaccggc
     1861 tgattgccgg ccgttgcctg attcgatcgg tcaggcgacg aggaatcatt tccacttcga
     1921 cgtcactgag tcccaaacgg cgcagttctc ctccgatgta tggagacgca gccaacatcc
     1981 gtcctgcatc atcggcacgc tggaacgaca gatatagccg acggtcggca gacccgcaga
     2041 tcaagctgaa gagcaggcgt tcctcgtcgt atcccgtcag tttttcatcg accttgaatc
     2101 cgagcgtggt gtccaatacc cgtcgatgac ggtcacgcag aaaagcgtct tcacgcacgt
     2161 atcggggaaa aaccttgtcg ttgaggccca ggacgaacaa ggctttgaat ggaagccctc
     2221 gggcggtcat cgcatccaat agcctcacac cctggtgcgg cgtaggctcc ataggaatgg
     2281 ccgcacgttc gaatgcatgt gttagcagtt ccgctaagtc catccatgtg atgtcttcac
     2341 caatcgcgtc gagttcgccg acggcggcgc aagcttgatc gacggcggtc catatcgcgc
     2401 tctcttcttc cgcacgatgc acatgcctgg ccatcaactc ccgaaacgca tgaatcaacg
     2461 acccaatcgt cccccgggca ggaagagtac tgtaagaatt caacagttct gagacggtct
     2521 cccataatag accgatgacg tcaggggcga ccttgaccga tccaatgatt ccggctgcgt
     2581 cgtcctcgac gtcccattcg agtgccgatt gactcgcttg ctccaatcgc ttccactcgt
     2641 caacgccatg ggtgatatgc agggcatcga gaaccagctt ccattgttca ggcctgtatg
     2701 agcgacaacg aacgaaccgg cctgaggaga ggggcacgat ataaaatggt gacgtcacga
     2761 gatcgagcac ggctgcacgg tagaagtcgt tggccggcag ggcagccagt tgaagcagcg
     2821 ttttacagag gggttcatga atgagcggcc gccgcgccgg agtggagaat ggaatccgat
     2881 gccgatcgaa gatgttccgc gcggcgctgt agtacggatc gagtgttcgg gctaccaccc
     2941 cgatctcatc aaaccgatac ccgtgcgttt cgaccagatc gaggatagtc cggcaggcaa
     3001 gtgcaagttc ctcttcctgg cctacgacgc ttcgcaatgc cacgtcgacg tgcgcaaagg
     3061 cctcgctcga atccgcagcc tcaaccaggt cgtcattcga tttgagcagc ggctgaacat
     3121 ggcggtcgaa gaagcgtcga gcaaaattga atgaagaatc tgtcgccaga ggaaaaaaga
     3181 gcgtggtggg aacggtcccg ctgaccgctt ggaacatgga gagttgcacc tgggttaagt
     3241 catagaagcc gtaataggag atgtgactca gcgacgtcag aaatggtgaa gcgggcactg
     3301 cagggagcac ggcttccgta aggtcatccg ccgtaacgac gtggaagccc tttcccactt
     3361 ccctgacagc agcgtgcagt gaaaacagcg cacgaagcca atccgcgtcg tcctgaccga
     3421 aatagccttc gcctgccgcg cggagggcgg tggccggatc taccccgccg tcgttcaagt
     3481 cccgcactgt cgaccaaagg cctgcccatg ttccgaacga acgtccgagt tgccgaagtg
     3541 caggcagctc cgccagccgc cgttgtacaa gatggcggac gaggtattcg aagaacagtt
     3601 cgtcgacgac acgcggtgga tgccctgtcc cttcgcgacg ggtctcctcc gctaatcgca
     3661 gagcgagctg atggaaggtc aaaaagtgga cgttgaccaa cgccagctgg tgctcaatgg
     3721 ccaacagcca tcggatccgg tcaagcagcg gcttcgatgg caccacaatg gcaatctgtg
     3781 accagacatc ggcgcctttg atccgttgga tatgattgac gagggcggac tcgagagagg
     3841 gctgaaaccg gccggtgacg acgcgtaaca ttcagcgacc gtgagggtgg cacatggcca
     3901 agtgggagag acccgtgcgg gcgtggtggg tcaacccacc acgcgctgac cgttgtgcac
     3961 tatcctgtct cgggacccag caattcgcca ttacagtcga cgcaggccca gtcaccgtcg
     4021 atgaaggaca tggggtcgcc gcagatcggg cagtcaggag gcggtccttt atcaattcga
     4081 ggcaggatcg gaagttcaat atcgtcttcg tccatcttta ccctgttgcg aatggcgaac
     4141 ttaacactac cctccctcgc aggaggttgt gtcttgcctt gtgtttgcct tgccggacgg
     4201 ccagttcgaa ctgccgccac catgtatgtg tttgaagtat ttcatactag ccttctcgcg
     4261 gccgcatttt cagttgcagg gccttttcgg cgctttctcg gctgggcacg ccgacgaacg
     4321 tcagccgccc atcgatgatc gtcgccggca cggcacgcac tgcatggcga ttggcaagtt
     4381 cctgtccgtc tggtgtcgta atgtccactt cgcggtacga aaaactgtac ttgacgcgga
     4441 gctgtttcca caggctcttt gccgacggac atgccccgca actgggagag atcaacaagg
     4501 tgatgttcgg catcgttgat tgggctcacg acgttcgagc atgaacacac tataaggact
     4561 cgcttcgaat gagacgctgg atcttacacc gtgggaaaag gcggtgcaag aagtgatgcg
     4621 agctggaagc ggctcagact agctggtcgc ccgacgaata cgcgaccagg acgcgcggaa
     4681 tgcgtgacgt gaatgatact aacgagtggc tgaaccggct ttcggctcct gatttccgga
     4741 tggagccggc ttgttcgttg cgctgttggt accgccaatc gcaggattgg gttgctccat
     4801 gttgcgagtc gacggatagt cctgagcgct gccttgtccc tgttgttgtc cgactggcgt
     4861 tgtttggtct tggtcacgat tacaagcgga cacgaggcta agtaccatga aagctcctgc
     4921 ggcggtgatg atcgttctac gagtcatgag tgctcctttg acataatatt tatcggccct
     4981 ccggccaagc ggtttgctga gtttccgtgc ctccgaccat tggaggaaag cccgaagggt
     5041 taccgccagg gttgccgggt gg
//
LOCUS       DASTQY010000295         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_303561, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000295 DASTQY010000000
VERSION     DASTQY010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303561"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VFR82_09755"
     CDS             <1..420
                     /locus_tag="VFR82_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU4501925.1"
                     /translation="STATIEYAMAVLKVPHIIVCGHTDCAVMKALLHPEEVQELPAVR
                     EWVGQAETTRRLMREHYTDLEGNARLIKTTQENVRAQLDHLRTHPSVALQLRRGKVDL
                     HGWVYSILTGDLWVYDFESEQFTPLVDEAPPRLKAAR"
     gene            515..1990
                     /locus_tag="VFR82_09760"
     CDS             515..1990
                     /locus_tag="VFR82_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEU4501926.1"
                     /translation="MGDRQLETLTFHNTYARLPEAFYAKLNPTPFSSSPYLVSFNAAA
                     ADLIDLDLREAERPEFTALFGGSMLTPAMEPLAMLYSGHQFGVYVPQLGDGRAILLGE
                     ATNARGERWDLHLKGAGLTPFSRDGDGRAVLRSTIREYLCSEAMHALSIPTTRALSII
                     GSDEKVYREQVESGAMLLRMAPSHVRFGTFEIFYYRRQHEHLRVLADYVIDYHYPHLR
                     ETPDKYAALFAQVVERTARLIARWQAVGWAHGVMNTDNMSILGLTLDYGPFGFMDDYD
                     AGFICNHSDHNGRYAFNQQPFIGLWNLSCLAQALLPLAEKDALKEGLDGYMPFFEQEY
                     RSLMRAKLGLADARDEDEELIREFLGLLQGSHADYTIVWRELGGFLSGRGMVNDRLRE
                     HFLNRERFDAWAVQYAERLRAERSRDDDRQQAMNRVNPKYVLRNYLAQAAIEKAQQKD
                     FAEIDRLLALLQHPYTDQPGMEGYAVPPPNWGKHLSVSCSS"
BASE COUNT          474 a          675 c          654 g          480 t
ORIGIN      
        1 agcaccgcga cgatcgagta tgcgatggcc gtactcaaag tgccacacat catcgtgtgc
       61 ggtcataccg attgcgccgt catgaaagcg ttgttgcacc cggaagaggt ccaggagttg
      121 cccgccgtga gggaatgggt cgggcaggcg gagaccacgc gacgcttgat gcgggagcac
      181 tataccgacc tagaaggcaa tgcccgtttg atcaaaacga cgcaggaaaa cgtccgcgcc
      241 cagttagatc atctcagaac gcatccctcc gtcgccttgc agctccgcag aggcaaggtc
      301 gatctccacg gttgggtgta ctccattctg accggtgacc tttgggtcta cgacttcgag
      361 agcgagcaat ttacgccgct cgtggacgag gcgcccccgc gcttgaaagc cgcccggtag
      421 aaccatccga cagcatcgtt ctcgcatcgc gccttccgac catgccaagg ctctccagtt
      481 gtaaactgcc cgtcagccct ggcatgctgg gtcgatgggc gatcgtcaac tcgaaaccct
      541 cacgtttcac aacacgtatg cccgtcttcc agaggcgttt tacgccaaac tgaatcccac
      601 ccccttctcg tcgtcacctt atctggtcag tttcaacgct gccgccgccg atctcatcga
      661 tctcgatctt cgagaagcgg agcggccgga gttcaccgcg ctcttcggtg gcagcatgtt
      721 gacgccggcg atggagccgc ttgccatgct gtattcgggt catcaattcg gcgtctacgt
      781 gccgcagctt ggcgacggtc gcgccatcct tctgggtgaa gccaccaatg cacggggcga
      841 gcgatgggat ctgcatctca aaggggcagg cctgacgccc ttctcccggg acggcgacgg
      901 ccgtgcagtg ctgcgttcga cgatacggga gtacctatgc agcgaggcga tgcatgcgct
      961 gagcattccc acgacgagag cgctctccat tatcggcagc gacgagaagg tgtaccggga
     1021 acaggtggag agcggcgcga tgctcttgcg tatggcaccc tcacatgtcc gattcggcac
     1081 cttcgagatc ttctattatc ggcgccaaca cgaacatctc agagtcttgg ccgactacgt
     1141 catcgactat cattaccctc acctccgaga gaccccggat aaatatgcgg cgttgtttgc
     1201 ccaagtcgtg gagcgcacgg ccaggttgat tgctcggtgg caagcagttg gctgggcgca
     1261 tggcgtgatg aacaccgaca acatgtctat ccttggtctg accctcgatt atggtccctt
     1321 cggcttcatg gatgactatg atgcgggatt tatctgcaat cactccgatc acaacggccg
     1381 ctatgccttc aaccagcagc cgtttatcgg attgtggaac ctgagctgtc ttgctcaagc
     1441 cttgttgccg ctcgcggaga aggacgcctt gaaggaggga ctcgatggct atatgccgtt
     1501 cttcgagcag gaataccgat cactcatgcg ggccaagctt gggctggcgg atgcacgaga
     1561 cgaagacgag gaactcatcc gtgaattcct gggtctgctc caaggcagcc atgcggacta
     1621 taccatcgtg tggcgagagt tgggcgggtt tctctccgga cgaggaatgg tcaatgacag
     1681 gttgcgcgaa cattttctca acagagaacg cttcgacgca tgggccgttc agtatgccga
     1741 gcggttgcga gccgagcgca gtcgggatga cgacaggcaa caagccatga accgtgtgaa
     1801 tccgaaatat gtgctccgca attacctggc ccaagcagcc attgaaaagg ctcagcaaaa
     1861 agacttcgct gagatcgacc gtctcctcgc cttgctgcaa cacccctata ctgaccagcc
     1921 cggaatggag ggatatgccg tccctccgcc caattggggc aaacacctca gcgtgagctg
     1981 ctcttcctaa atacggccct tcctccgttc tgtcagttgt gcatacattc cacggtcgag
     2041 tgccttgttg cagtccccct cattgtctgg tgtacagtgg tccgattttt gcgcgaataa
     2101 ggagaagcca tccgtggcca agcctcgagg agattcaaaa ctagcggtcg caggcgcact
     2161 cacgctggta ctggccattg ccggcgtgtt gttggtcaaa gagcccctgc gaagctcacg
     2221 acccgtgggt agcggactgg agatgaagca gtccaccgcc gagcagttag tcagggcgcg
     2281 ctt
//
LOCUS       DASTQY010000296         5063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1422130, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000296 DASTQY010000000
VERSION     DASTQY010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422130"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VFR82_09765"
     CDS             <1..474
                     /locus_tag="VFR82_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEU4501927.1"
                     /translation="HGGANEEVVQMLRDIGSAGQARHYVEEQVRKKKKLMGFGHRVYK
                     VKDPRAIILQNLCDMLFKTCGTSPLYDIALAVERAAGETLHGKAVYPNVDFYSGIIYD
                     KMGIDTDLFTPIFAMARVSGWLAHWLEQLRENKLFRPDQIYAGEHNRPYVPVDQR"
     gene            673..1119
                     /locus_tag="VFR82_09770"
     CDS             673..1119
                     /locus_tag="VFR82_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU4501928.1"
                     /translation="MTLVRWDPFRELEDMSERLNRVFSRPTLRNTGKENLTVADWMPT
                     VDISESESEYLIKAELPEVRKEDVKVTVENGVLTLQGERRQEKEEKGKRFHRVERSYG
                     SFVRSFTLPESVDEGGVRAEYKDGVLALHLPKSEKVKPKAIDVKVA"
     gene            complement(1179..1418)
                     /locus_tag="VFR82_09775"
     CDS             complement(1179..1418)
                     /locus_tag="VFR82_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501929.1"
                     /translation="MDSPHLTVDAFAGYKGEKTPRSFVLEGRILSVQHIVDRWYSETH
                     SYFRIKACDGQRYVLRLDLDEDVWELIMSEPIEEP"
     gene            1499..2938
                     /locus_tag="VFR82_09780"
     CDS             1499..2938
                     /locus_tag="VFR82_09780"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEU4501930.1"
                     /translation="MKNVASQKSSLPTRMERDTMGELAVPAEAYYGVQTARAIENFPI
                     SPLRIPRSVIRAMGMIKRAAASVNHSLGLLDKKQADAIVEAATEVIEGKLDAEFPVDI
                     FQTGSGTSTNMNTNEVISNRATELLGGVRGSKLVHPNDHVNLGQSSNDVIPTAIHIAA
                     SETIHRQLLPALSSLHSALDRKASEFDGIVKIGRTHLQDATPVRLGQEFGGYARQIEL
                     GIGRIKRAQEALSEVALGGTAVGTGLNCHPDFSSHVLTIISRETGCPFTEAGNHFEAQ
                     SSQDSLVEASGALRTLAVSLTKIANDIRWLGSGPRCGLGEIHLPETQPGSSIMPGKVN
                     PVIAESVIMVCAQVIGNDLTVTIGGQAGNFELIVMLPVMAYNLLQSIELLAAASNNFA
                     AKCIEGITANEERCKSLIEQSLAMCTALAPEIGYEAAAKLAKEAYRSGKTVREVAREH
                     QVLTETRLNELLDPWRMTEPGGPVGSAGG"
     gene            3034..4386
                     /locus_tag="VFR82_09785"
     CDS             3034..4386
                     /locus_tag="VFR82_09785"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4501931.1"
                     /translation="MSTQHKHVIIVAGAGPAGMAVAASLGKAGHEVIILNRDIKFGGL
                     AEYGIFPSKLKLRGGLRKQYWDLLQQPNVHYFGNVSIGKDKDLTVDDVRSLGASAVVF
                     SIGAQGTKAIGVEGDSAQGVFHAKDVVYHFNRLPGFGDRPFEMGKHVAVIGAGDVMVD
                     IAHWLTRYKKVERVTAIVRRGPAERKYNPKEIRAVCANMDLEGIKKEFDRIKDRLVAV
                     GQNPEEIQKGLLDEFTKCEPAASRTKMGFRFLASPKRILVNEHNRVRGLEMEDNKLEP
                     KGEDTAAVGLKQFYEFSCDSVVFAVGDKVDDTVGLPYKGGTFLTNPNKTGNDPDDALF
                     QAYDESSGKILEGLFLAGWARKASEGLVGVAKRDGDWCAEVVTRYLATKAAGNTVKAV
                     LEKLQALLKERKGRPVDIKGLRALEAAEKAHKDMTDCIGEFKYVTNQEMLSLIERGSR
                     "
     gene            complement(4397..>5063)
                     /locus_tag="VFR82_09790"
     CDS             complement(4397..>5063)
                     /locus_tag="VFR82_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEU4501932.1"
                     /translation="LGGDGTMLNAARLAAERGIPILGVNMGGLGFLTEVRLEHLFPSL
                     ERVFANEYVLDERLMISSHIHRHGETVAQGLVLNDVVISKGTLARMIELNIAIQGQFV
                     TTLRGDGLIVSTPTGSTAYSLSAGGPIISPAVRSLILSPICPHTLTHRPLIVPDNVEI
                     DVTLTSKDEGAMATLDGQVGVALTQGDTVLLKVSEHRTRLIRFPESSYYAVLREKLKW
                     GGG"
BASE COUNT         1230 a         1344 c         1469 g         1020 t
ORIGIN      
        1 catggaggag cgaacgaaga agtggttcag atgctgaggg acatcggatc ggctggtcag
       61 gctcgtcact atgtcgagga acaggtgcgt aagaaaaaga agctcatggg tttcggacac
      121 cgggtctata aggtgaagga cccccgggcg ataatcctgc aaaatctctg cgacatgctc
      181 ttcaagacct gcgggacgtc accactctac gacatcgctt tggcagtcga gcgtgccgcg
      241 ggcgagacat tgcacggaaa agcggtgtat ccgaatgtcg atttctattc cggcattatc
      301 tacgacaaga tgggaatcga caccgacttg ttcacgccga tcttcgcgat ggcccgggtg
      361 tccggatggc tcgcccactg gctggaacaa ttgcgggaaa ataagttatt ccgccctgat
      421 caaatctacg ccggagagca taaccgcccc tatgtgcccg tcgaccagcg ttgataattg
      481 ggcccatcca cccttgacac aattggtcac cggagaggca agccgtagat tttcagttca
      541 tcggggaagt ggagaagtcc gcttgcccgc ctcttgactc ggcgagctcc cgtcttatct
      601 gtgttctagg cgaaaaccgg cgcagcgcca aagctgaaca cagagtaaag cgacaggaca
      661 aggaggtgga ctatgacact cgtacggtgg gatccttttc gtgaattaga agatatgtct
      721 gagcgattga accgcgtgtt ctctcggccg acgttgagga ataccggcaa agaaaatctc
      781 acagttgccg actggatgcc gacagtggac atcagcgaaa gcgaatcaga atacctgatt
      841 aaagccgagc tgcccgaagt gaggaaggaa gacgtgaagg tcacggtgga gaatggggtg
      901 ctgaccttgc agggggaacg tcgacaggaa aaggaagaga aggggaagcg gtttcatcga
      961 gtggagcgct cgtacggaag ctttgtccgg agtttcacgc ttcccgaatc cgtggacgaa
     1021 ggcggcgtca gggccgagta caaggacggc gtcctcgcgt tgcatctgcc aaagtcggaa
     1081 aaggtgaaac caaaagcgat cgatgtaaag gtggcctaaa acgattcgcg agaaggcccg
     1141 ctccggcggg ccttcgtctt tacggagtaa gtccgccctc acggttcttc gatcggttcc
     1201 gacatgatca actcccaaac gtcctcatct aagtccaacc gcagtacata cctttgcccg
     1261 tcacaagcct tgatcctgaa atagctgtgc gtctcgctgt accagcgatc cacgatgtgt
     1321 tgaaccgata gaatccggcc ttcgagcacg aaagaacgcg gcgtcttttc gcctttatat
     1381 ccggcaaaag cgtcaacggt gagatgaggg ctatccatca ctgactcttg tagaagcagg
     1441 tgacctgagt ttatcatggc gcccgcagct tgaaagcctt ccaagcatgg agccgcgcat
     1501 gaagaacgta gcttctcaga agtcgtcact gcccacaaga atggagcgcg acacgatggg
     1561 cgaattggcc gttccggctg aggcctatta tggcgtacag acggcgcgcg caatcgaaaa
     1621 ttttcccatc agccccctgc gcatcccccg atctgtcatc agggcaatgg ggatgatcaa
     1681 gcgggctgcc gcttccgtga accattcgct ggggctgctc gacaagaaac aggccgacgc
     1741 gatcgtagag gcggcgaccg aagtcataga ggggaaactc gacgcagagt ttcctgtcga
     1801 tatttttcag accggctcag gcacctcgac gaacatgaat accaacgaag tgatttccaa
     1861 ccgtgcgacc gaattgctcg gtggcgtccg aggcagcaag ctcgtccatc ccaacgatca
     1921 cgtcaatctc ggccagtcta gcaacgatgt cattccaacg gccattcaca tcgctgcctc
     1981 tgaaacgatc catcgtcaat tgctgccggc gttgagcagc ctccatagtg ctctcgatcg
     2041 gaaggctagc gagttcgatg gtattgtcaa gattgggaga acccatttgc aggacgcgac
     2101 ccctgtgcgg cttggacagg aattcggcgg gtatgcccga cagattgaac ttggcattgg
     2161 acgtataaag cgtgcccaag aggccctgag cgaagtggcc ctcgggggta ccgccgtcgg
     2221 cactggattg aattgtcatc ctgacttctc ctcgcatgtg ctcaccatta tatcccgtga
     2281 aacagggtgt ccattcacag aggcggggaa tcatttcgaa gcccagtcgt cacaggactc
     2341 cctggttgag gcgagcgggg cattgcgaac gttggcggtg agcctcacaa aaatcgcaaa
     2401 tgatattcga tggctcggat ccggaccgcg atgtggcttg ggagaaatcc atttacccga
     2461 aacacaaccc ggatcctcca tcatgcccgg aaaggtcaat cccgtgatcg ccgaatcggt
     2521 gatcatggta tgcgctcaag tcatcggcaa cgacctcacg gttaccatcg gcggacaagc
     2581 cgggaacttc gagttgatcg tgatgctgcc agtgatggcg tacaacctcc tgcaatccat
     2641 cgaactactt gcggctgctt ccaacaattt cgccgccaaa tgcatcgaag gaatcaccgc
     2701 gaatgaagaa cgctgcaaaa gtctcatcga acaaagcctt gccatgtgta cggccttggc
     2761 tcctgaaatc ggctacgagg cggcagcgaa actggcgaag gaggcgtacc gatcaggcaa
     2821 gacggtgcgg gaggtagcga gagagcacca ggtcttgacc gagacacgcc tgaacgaact
     2881 attggacccg tggcggatga ccgaacccgg cgggccggtg ggaagcgcgg gcgggtgaaa
     2941 cgaccggggc tcggttttga cagcgtcgaa accgctatgc taaagtccgc gtaactgtta
     3001 gctttctcgt cattttttca caatcgggta ggtatgagca cacagcacaa gcatgtgatt
     3061 attgtcgccg gtgcaggtcc ggcgggtatg gccgtagctg cctcgctggg gaaagccggt
     3121 cacgaagtta tcatcttgaa tcgggatatt aaattcggtg gactcgccga gtatgggatc
     3181 ttccccagca agctgaaact tcgcggcggt ctccggaaac agtactggga cctcctgcag
     3241 caaccgaacg ttcattattt cggcaacgtg tcgatcggga aggacaaaga tctgactgtc
     3301 gatgatgtcc gctcactggg cgccagtgca gtggtgtttt caatcggcgc acaggggacc
     3361 aaagccatcg gcgtagaagg ggattctgca cagggcgtct tccatgccaa agacgtcgtc
     3421 taccatttca accggctccc gggctttggt gatcgtccgt tcgaaatggg caagcacgtc
     3481 gcggtcatcg gcgccggcga tgtcatggtg gacatcgcac actggctgac tcggtataaa
     3541 aaagtcgaac gcgtaaccgc gatcgtgcgg cgaggtccag cggagcggaa atacaatccg
     3601 aaggagatcc gtgcggtgtg cgccaatatg gatctggaag gcatcaagaa agagttcgac
     3661 cggatcaagg atcggctcgt ggccgtcggc caaaatccgg aagagataca aaaagggctt
     3721 cttgatgagt tcacgaaatg cgagcctgcg gcgagtcgga ccaaaatggg attcagattc
     3781 ctggcttcac ccaagcgcat ccttgtcaac gaacataatc gtgtccgtgg ccttgaaatg
     3841 gaagacaaca aactggaacc caaaggcgag gacacggccg ccgtgggact caaacagttt
     3901 tacgagttct cttgcgactc ggtcgtgttc gctgtcggcg acaaggtcga cgacacggta
     3961 ggattgccct ataaaggggg tacttttctg accaatccca acaagactgg caacgaccct
     4021 gatgatgcgc tctttcaagc ctatgacgaa tcatccggca aaatcttgga aggactcttc
     4081 cttgccggtt gggcaagaaa ggcgagtgaa ggcttggtgg gggttgcaaa gagggatgga
     4141 gattggtgcg ctgaagtcgt cacccggtat ctggcgacca aagctgcagg caacaccgtg
     4201 aaggctgtct tggagaaact gcaggcgttg ctcaaagaac ggaaggggcg gcccgtcgac
     4261 atcaaaggtc tccgtgccct cgaagcagcc gagaaagcac acaaagacat gacggactgc
     4321 attggagaat tcaagtacgt gacgaaccag gaaatgctta gcctgatcga gcgcggcagc
     4381 agataacctg acccgtttaa cctccgcccc atttcaactt ttcacgcagg actgcgtagt
     4441 agctgctttc cgggaaacga atcaaccgtg tccggtgttc agagactttc aaaagcaccg
     4501 tatccccttg ggtcagtgcc acacctacct gcccatcaag cgtggccatg gcgccttcat
     4561 ccttgctggt taatgtcacg tcaatctcga cgttatctgg aacgataagg ggccggtgag
     4621 tgagcgtatg gggacagatc ggcgagagga tcaaggagcg gaccgccgga ctgataatgg
     4681 gaccgccggc agagagggaa taggcggtgg atccggtggg tgtgctgaca atgagtccat
     4741 caccgcgaag cgtggtgacg aattgtcctt gaatggcaat gttgagctcg atcattcgcg
     4801 cgagcgttcc cttgctgatc accacatcat tcagcacaag gccctgagcc accgtttcac
     4861 catgccgatg aatgtgcgaa ctgatcatca gccgttcatc gagcacatat tcattagcaa
     4921 atacccgctc cagggaaggg aacaagtgtt cgagcctgac ttcagtcaga aacccgagtc
     4981 cacccatgtt gactcccagg atcggaatcc cccgttcggc ggctaaccgc gctgcattga
     5041 gcatggtgcc gtcgccgccc agg
//
LOCUS       DASTQY010000297         4208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1293509, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000297 DASTQY010000000
VERSION     DASTQY010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293509"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VFR82_09795"
     CDS             complement(<1..1232)
                     /locus_tag="VFR82_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEU4501933.1"
                     /translation="MASRIYEWLDSRLKIASLEKTLLDEPIPGGASWIYVFGSATLFL
                     FLLQGITGMFLAVYYTPSPDHAYDSVQFIEQQVTFGWFVRGLHHWGASGMVIAIGLHM
                     LQVFLYGAFKPPREMMWMVGVVLFLLVMAFAFTGYLLPWDQTAYWATQVGINMVGTVP
                     LVGDFLVRVMRGGETLGALTLSRFFAVHVLFLPALIVLGIVVHLFILRRVGPAGPWDE
                     RKAAAAREPFYPRQVYMDAVVMVGVFLVLVVLARFVPFPLADKANPADTSFVPIPEWY
                     FLFYYELLKYIHGPLEPLATWVLPAAVILVMLLWPFLDRQKHVRAPASRPVGMAIAAV
                     FLVTVFSLLGISLKNLYGMKRTDPAIAHGQALYANLGCAGCHRIHGNGGAIGPDLSYE
                     GDARSDREWHIKHFRDPQS"
     gene            complement(1634..3142)
                     /gene="nirK"
                     /locus_tag="VFR82_09800"
     CDS             complement(1634..3142)
                     /gene="nirK"
                     /locus_tag="VFR82_09800"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HEU4501934.1"
                     /translation="MTSATRRNAMIPDARLFSRWSLWTLSCAMAMTSTTVMTADAAPT
                     TEAHSAKAKGGSSQRKSTAQSGGTIPAILTDAPQVPPRITRRHPSKIIIELEVSEVNL
                     PMADGVEYTFWTFGGKVPGKFIRVRQGDTVEFHLMNHPESRFPHNIDLHAVTGPGGGA
                     ASSNTAPGRRTQFTFKALNPGLYVYHCATAPVGEHVANGMYGLILVEPPGGLAPVDRE
                     YYVVQSDFYTKGPHHEKGHQPFDMDKAIEEHPTYVVFNGAEGSLLGDNALKAKVGERV
                     RIFVGNGGPNLVSSFHVIGEIFDRVYAEAGTRYSEHIQTTLVPAGGAAIVEFKVDVPG
                     SYKLVDHSILRAFNKGALGELKVEGPEAKLIYSGKQAESEYQAKGTPAGPASTSPMKA
                     PGAAGETLYKTYCMGCHQAEGEGMPGTFPPLAKSDYLMADASRVIETVLNGLTGSIQV
                     NGQAYNGTMPPMGHLKDEEIANVLSYVMTSWGNAANPVSAAEVAAVRAKTSH"
     gene            complement(3183..3731)
                     /locus_tag="VFR82_09805"
     CDS             complement(3183..3731)
                     /locus_tag="VFR82_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEU4501935.1"
                     /translation="MTFSPEPHRAGGTSVSPDGARRRFFQWVIQASAGFIGLSLAIPF
                     VGYLIGPALKRRTQQWVDVASTLEFTTGVPKQLEYVATVQDGYLETKTQKAVWAVKQA
                     NGEVTVFAPMCPHLGCGYHWDGAGQQFKCPCHGSVFDISGRVVAGPAPRPLDVLPAKI
                     DNGRLLVVFKEFKSGVAKQIEL"
     gene            complement(3728..>4208)
                     /locus_tag="VFR82_09810"
     CDS             complement(3728..>4208)
                     /locus_tag="VFR82_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501936.1"
                     /translation="VAASNDPLDGARALEKASKSSLPKTQTTTVNGLPAARTQVGDSR
                     LTADLTWIAYGGMVYQIAGIAETRTFDAVRPVFQTVVQSFRPLSTDERNGIREKRLRL
                     VKAQAGETVEALTARSHSVWKASQVTVANGFKGSEVLKDGQLVKIAVEEPYAGKPQR"
BASE COUNT          895 a         1263 c         1166 g          884 t
ORIGIN      
        1 gattgcggat cacggaagtg cttaatgtgc cattccctgt ctgaccttgc gtccccctcg
       61 taggacagat ccggtccaat cgctcctccg ttgccatgaa tgcgatgaca tccggcacag
      121 ccgagattcg catagagcgc ctgaccatgt gcaatcgcag gatcggtgcg cttcatccca
      181 taaagattct tcaacgagat gcccaggagg gagaagacag taacgagaaa cacagcggct
      241 atggccatcc ccacggggcg cgaggccgga gcacggacat gtttttgccg gtcgagaaac
      301 ggccagagca gcatcaccag gatgacggcg gccggcaata cccacgtcgc caacggttcc
      361 agcggaccat ggatgtactt cagcagctca taatagaaga ggaaatacca ttccggaatc
      421 gggacaaagc tggtgtccgc tgggttggct ttgtcggcca gtggaaacgg cacgaaccgc
      481 gccaacacta ccaggacaag aaataccccg accatcacta ccgcatccat gtagacttgc
      541 cggggataaa aaggctccct ggctgccgct gccttccgct catcccaggg accggccggt
      601 ccgactcgcc gaaggatgaa cagatgcacg acaatcccca gtacaataag ggccggtaaa
      661 aacagcacat gcacggcaaa aaatctcgag agggtcaagg cgcccagcgt ctctccgcca
      721 cgcatcaccc tgacgagaaa atcgcccacg agcgggaccg tgcctaccat attgattccc
      781 acctgggtgg cccagtaagc agtttggtcc caaggaagta aatacccggt gaaggcaaac
      841 gccatcacca gcagaaacag cacgacgccc accatccaca tcatttctcg tggtggtttg
      901 aacgcgccgt agaggaacac ttgcagcatg tgcaggccga tggcaatcac catccccgat
      961 gcgccccagt gatgaagccc tcggacgaac cagccaaacg tcacctgctg ctcgatgaac
     1021 tgcacgctgt catacgcatg atcgggcgag ggtgtgtaat acacggcgag aaacatgccg
     1081 gtgataccct ggaggagaaa gagaaagagc gtggcggagc cgaagacata gatccagctg
     1141 gctccgccgg gaatgggctc gtccaacaat gttttttcga gagacgcgat tttcagccgg
     1201 ctatccagcc actcgtagat ccgggacgcc atcgcagcgc tcctaaatgc cgttgactga
     1261 cgttactttc tgaacgtcaa aacatagccc atgaggtcgc tcagctctgc ttgagacagc
     1321 tggctcttcc aagtcccatg gctgtgccag gttttccgat ttcgacgacc tgcctcaagt
     1381 cagcctccgg cttctttttg ctttcagcgc tcgtgaagtc ggcggctgga ggctagagca
     1441 tcctcccagc gggaccgtct ccctttccta gtgggcggtg atagacaagg cagtgcttct
     1501 catagaggac ctttcccttt tcacgatcac cagccgcatc gacggtacac aggtgagaca
     1561 acaccgatcc caagagcgcg ctcgtacata gcactgccgc ataccgaacg cctgtcatcg
     1621 tgtgttctac tcgttaatgt gaggtcttgg cccgcacggc cgccacttct gccgccgaga
     1681 cagggttcgc agcgttcccc caagaagtca tgacgtaact caagacgttg gcgatttcct
     1741 catctttgag atgccccatc ggcggcatgg tgccgttgta ggcttgcccg ttcacctgaa
     1801 tagatcccgt gaggccgtta aggacagtct cgatcacccg tgatgcgtcg gccatcaaat
     1861 aatccgattt tgcgagcggc ggaaacgttc ctggcatccc ctccccctca gcttgatggc
     1921 aacccatgca ataagtcttg tagagagttt cacccgcggc tcctggtgct ttcatcggcg
     1981 atgtggaggc cgggccggca ggggttccct tcgcctgata ttccgattcc gcctgcttcc
     2041 ccgaataaat cagtttggcc tccggccctt cgaccttcaa ctcaccaagc gcccctttgt
     2101 tgaacgcccg caggatggaa tggtccacga gtttgtagga accggggacg tccaccttaa
     2161 actccacgat agctgctcct cctgccggga caagcgtggt ttgaatgtgc tcagagtagc
     2221 gggtaccagc ttctgcatag acacggtcga agatctcgcc gatgacatgg aaactcgaga
     2281 ccagattcgg tccgccgttt cccacaaata tccggacacg ctccccgact ttggctttca
     2341 gcgcgttgtc acccaacaag gatccttcag cgccgttaaa tacaacatag gtcggatgtt
     2401 cctcgatggc tttgtccatg tcgaaaggtt ggtggccttt ctcgtggtgc ggtcccttcg
     2461 tataaaaatc gctttgcacc acatagtatt cacgatccac tggagccagc ccccctggcg
     2521 gctcaaccag gatcaggccg tacatgccat tggcgacatg ctcgccgact ggcgcagtgg
     2581 cgcagtgata cacatagaga cctgggttga gcgccttgaa tgtgaattgc gtgcggcggc
     2641 ccggcgcggt attgctgctg gcagctccac cgcctgggcc ggtcaccgca tggaggtcga
     2701 tgttatgtgg gaaccggctc tcgggatggt tcatgagatg gaattcgacc gtatcgcctt
     2761 gccgcactcg aatgaatttt ccggggactt ttccaccgaa ggtccagaag gtgtattcaa
     2821 caccgtctgc cataggcagg ttgacctcgc tcacctctag ttcgatgata attttgcttg
     2881 ggtgacggcg tgtaattcgc ggcggcactt ggggggcatc cgtcaggatg gccggaatcg
     2941 tgccgccgct ttgggcggtc gatttccttt gagacgaacc gcctttggct ttcgcactgt
     3001 gcgcctctgt cgttggagcg gcatcggccg tcatgacggt agtcgaggtc atggccatcg
     3061 cgcacgacag cgtccagaga gaccatcggc tgaacaagcg agcgtcaggg atcatggcgt
     3121 tccggcgcgt cgccgatgtc atccattggt cctctcatgc tagagcgggc tcactttgtc
     3181 cattacagtt cgatctgttt cgcaactccg gacttgaact ccttgaacac cacaaggaga
     3241 cgaccgttat cgatttttgc aggcaagaca tccagcggcc gaggcgccgg ccccgcaacc
     3301 acacgtcctg agatgtcgaa cacgctgcca tggcaggggc atttgaactg ttgccctgcg
     3361 ccatcccagt gatagccgca ccccaaatgc gggcacattg gagcaaacac cgtcacctcc
     3421 ccgttggctt gctttacggc ccacacggct ttttgcgtct tggtttcaag atagccgtcc
     3481 tgaaccgtcg ccacatactc caactgtttg gggacaccgg tcgtaaactc gagcgtggaa
     3541 gccacgtcca cccactgctg cgtccgtcgc ttgagcgctg gaccaatgag atagccgacg
     3601 aatggaatcg ccaggctgag gccgataaac ccggctgatg cctgaatgac ccattgaaaa
     3661 aagcgtcggc gcgcaccgtc ggggctgacg gatgtccccc cggcacggtg gggttcagga
     3721 gaaaatgtca tcgctgtggt tttccggcat agggttcttc gacggcaatc ttgaccaact
     3781 ggccgtcctt aagaacttcc gatcctttga agccattggc gacggtcacc tggctggcct
     3841 tccagacgga gtgggagcga gcggtcagcg cctccaccgt ttcgcctgct tgggccttca
     3901 cgagacgaag ccgtttttcc cggatgccgt tgcgttcgtc ggtagaaaga gggcgaaagc
     3961 tttgcacgac tgtttggaac accggtcgga ccgcgtcaaa cgtcctcgtc tctgcaattc
     4021 ccgcaatctg atagaccatg cccccgtagg caatccacgt gagatctgcg gtcagtcgcg
     4081 agtcaccgac ctgggttcgt gccgcaggaa gaccattgac agttgtcgtc tgcgtcttgg
     4141 ggagcgagga cttgctcgcc ttctccaagg cacgtgcgcc atcaagagga tcgttactcg
     4201 cggcgacg
//
LOCUS       DASTQY010000298         4609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_21648, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000298 DASTQY010000000
VERSION     DASTQY010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21648"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /gene="trpA"
                     /locus_tag="VFR82_09815"
     CDS             complement(<1..530)
                     /gene="trpA"
                     /locus_tag="VFR82_09815"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU4501937.1"
                     /translation="MRSTGRRARAPITNQSVRGTSRLDQTFARLKANREKALIAYLMA
                     GDPGLAETEELVLALEKAGADIIELGVPFSDPIADGPVIQQAAERALRSGTSLRKILL
                     MVAAIRIKSQIPLVLMAYYNSIHAYGPDRFCQEATAAGVDGLIVPDMPPDEAGPLRGP
                     ACSSGLQLIYLLAPTST"
     gene            complement(530..1744)
                     /gene="trpB"
                     /locus_tag="VFR82_09820"
     CDS             complement(530..1744)
                     /gene="trpB"
                     /locus_tag="VFR82_09820"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU4501938.1"
                     /translation="MNTKLPRPATIPDQQGRFGAYGGRYVPETLMPALLELEREYAKA
                     RQDRAFQGQFAYYLKEYVGRPTPLYYAERLTRRLGGAKIYLKREDLCHTGAHKINNAI
                     GQGLLAKRMKKPRIIAETGAGQHGVATATVAAMFGLQCEIYMGTEDMQRQALNVFRMR
                     LLGSTVTGVEAGSRTLKDAISEAMRDWTTNIRTTHYILGSVLGAHPYPMMIRDFQSII
                     GKEARKQILAAEGRLPDCLMACVGGGSNAIGLFHAFLHDKKVRMIGVEAGGLGLASGK
                     HAARFSGGKPGVLQGTMTYLLQDEDGQVNLTHSVSAGLDYAAVGPEHSYLREIGRTTY
                     TSVTDDEALAAFDLLAREEGIVPALESAHAIAHAVHIAPKMKRNQILLINLSGRGDKD
                     VQQVARMRGISL"
     gene            complement(1765..2406)
                     /locus_tag="VFR82_09825"
     CDS             complement(1765..2406)
                     /locus_tag="VFR82_09825"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU4501939.1"
                     /translation="MSMGLKVKICGITNAEDASVAVAAGADALGFIFYEKSPRYIVPA
                     VAARIIAGLPPLVTAVGVFVNEGLATVRSIMDTCGLALAQLHGNEDATYCRELSRPAM
                     KALRLKDRNSFLSLAEYQGRAGVRGFVIDTFSELAYGGTGQVADWTLASEAAKTASIL
                     LAGGLTPGNVADAIRAVRPYGVDVSSGVESIPGKKDHQKVRAFVDAVRVVSSS"
     gene            complement(2410..3231)
                     /gene="trpC"
                     /locus_tag="VFR82_09830"
     CDS             complement(2410..3231)
                     /gene="trpC"
                     /locus_tag="VFR82_09830"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU4501940.1"
                     /translation="MILARILEHKKAEVRHKQSRGYLADLRAEIRDISRPLGFSVALE
                     ATRSSVSPALIAEVKKASPSLGLLRPEFEDKFDHVAIARCYEESGASALSVLTDKDFF
                     QGSLDYLRAIKRSVALPMLNKEFMVEEIQFYEARAYGADAVLLIVAALERRQLVDFHA
                     LAGELLLDVLIEVHHERELETVLEWIPNARMIGINNRDLTTFSTDLTVTKRLAPRIPS
                     DRIIVSESGIQNRGHVEQLLEMGVHAMLVGESLIRADDVRTKIQELRGLRSAAQA"
     gene            complement(3228..4256)
                     /gene="trpD"
                     /locus_tag="VFR82_09835"
     CDS             complement(3228..4256)
                     /gene="trpD"
                     /locus_tag="VFR82_09835"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU4501941.1"
                     /translation="MAMIRDAIAKLADRLSLTEKEAEEVMNQIMEGIATPAQISAYLM
                     GLRLKGETVEEIAGSVRAMRARAVRIAVGAPLVIDTCGTGGDGRHTFNISTTTAFVVA
                     GAGLTVAKHGNRSVSSKSGSADVLSELGVNISLSPDLVSECINEVGIGFLFAPLYHGA
                     MRHCAGPRQEMGIRTMLNLLGPLTNPAGATVQVLGVYEPRLTELLGKVLMHLGSQHCF
                     VVHGMDGLDEITLTERTIVSEAKAGVLSNYVLTPHEFGLNRVSPKELAGGSPQDNARI
                     TREILQGRKGAKRDVVCLNAAPALVAGKKAKTLQEAYRLAGDLIDSGAAAEKLARLVS
                     FTSKGAHA"
     gene            complement(4265..>4609)
                     /locus_tag="VFR82_09840"
     CDS             complement(4265..>4609)
                     /locus_tag="VFR82_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248583.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU4501942.1"
                     /translation="PLLGVCLGHQSLAVAFGGEVVRAVRLMHGKTSPIQHDGRTIFRQ
                     LPNPFDATRYHSLIVNRSSLPACFEVSAETAEGEIMGMRHRSLGVEGVQFHPESILTA
                     AGKDLLRNFLKL"
BASE COUNT          945 a         1348 c         1326 g          990 t
ORIGIN      
        1 gtgctggttg gggcgagtaa atagatcagt tgcagtcccg acgagcaggc cggtcctcgc
       61 agcggacccg cttcatcggg aggcatgtcc gggacaatca aaccgtccac tccggccgcc
      121 gttgcttcct ggcaaaatcg gtccggcccg tacgcatgga tgctgttgta ataggccatc
      181 agaacaaggg gaatctgtga tttgattctg atggccgcaa ccatcaaaag gattttgcgc
      241 aaggatgtgc cgcttcggag cgcccgttct gctgcctgtt ggatgaccgg gccatccgcg
      301 atgggatcgg aaaacgggac gcccaactcg atgatatcgg cgccggcttt ttccaatgcg
      361 agaacgagct cttcggtttc ggccaatccg gggtcgccag ccatcaaata ggcaatcagg
      421 gccttctcac ggttagcctt cagccgcgcg aatgtctggt cgagccggct cgtgcccctc
      481 acagattgat tcgtgatcgg cgctcgagca cgacggccag tgcttctcat cacaaggata
      541 ttcctctcat gcgtgcgact tgctgcacgt ctttgtcccc tcgcccggag aggttgatca
      601 ggagaatctg atttcgtttc atcttgggag cgatgtgaac cgcatgcgcg atggcatggg
      661 cgctctcaag tgctgggacg ataccttcct ccctggcgag caagtcgaaa gcggccagcg
      721 cctcgtcatc ggtgactgaa gtataggtgg tcctgccaat ttcgcgtaaa tagctgtgtt
      781 ccggcccgac ggctgcataa tccagtcccg ccgacactga atgagtcagg ttgacctggc
      841 catcctcatc ctggaggaga tacgtcatgg ttccttgcag cacgcccggc ttacctccag
      901 aaaaacgggc cgcgtgcttt ccgctggcaa gcccaagccc tccggcctcc acgccgatca
      961 tccggacctt cttgtcgtgt aagaacgcat gaaacaatcc gatggcgttg ctgccgccac
     1021 cgacgcaggc catcaggcag tccggtaatc gcccttcggc tgccagaatt tgcttgcggg
     1081 cttccttgcc gatgatcgat tgaaaatccc ggatcatcat cggataggga tgtgcgccga
     1141 ggaccgaacc aagaatgtag tgcgtcgtgc ggatattcgt cgtccagtct cgcatcgctt
     1201 cgctgatggc gtctttcaac gtgcggctgc cggcctccac gccggtcacg gtcgacccaa
     1261 gaaggcgcat gcggaaaacg ttgagcgcct gacgctgcat gtcttccgtg cccatataaa
     1321 tttcacattg caatccgaac atcgcagcga cagtggcagt cgcgacgccg tgctggcctg
     1381 ctccagtctc ggcgataatg cgcggcttct tcatgcgctt ggccaatagg ccctgtccga
     1441 tggcattatt gattttatgg gcaccagtat gacaaagatc ctcccgcttc aaataaatct
     1501 tggcaccgcc tagacgtctg gtcagccgtt cggcatagta gagcggcgtc ggccgcccga
     1561 cgtattcctt gaggtaatag gcaaattgtc cctggaaggc gcgatcctgc cgcgcttttg
     1621 cgtattcccg ctccaattcc agcaaagcag gcatgagcgt ttcgggcaca tatcggccac
     1681 cataggcgcc gaagcggccc tgttgatcgg gaatcgttgc cgggcgggga agtttcgtat
     1741 tcacgtcggg cagtataaac agcctcagga agaagagaca accctcacgg cgtcgacaaa
     1801 ggcgcggact ttttgatggt ctttttttcc tggaatcgat tcgacaccgc tgctgacatc
     1861 cacaccataa ggtcggacgg cacgaatggc gtcggcgaca ttgccagggg tgaggccgcc
     1921 tgcgagcagg atggaggcgg tttttgcggc ttctgacgcg agggtccagt ctgccacctg
     1981 accggttcct ccgtacgcca gctctgaaaa ggtatcgatg acaaatcccc gtacgccggc
     2041 ccggccctga tattcagcca atgacagaaa gctgttccga tcctttaagc gcaatgcttt
     2101 catggccgga cgagatagtt cccggcagta ggtcgcgtct tcattcccat gcagctgagc
     2161 gagagcaagg ccgcaggtat ccatgatcga ccggaccgtc gcgagtccct cattgacgaa
     2221 gacgccgaca gcggtgacca acggaggcaa tccggcgatg atccgggcgg cgacggcagg
     2281 cacgatatac cgggggcttt tctcataaaa aatgaacccc agcgcgtcgg ccccagccgc
     2341 tacggcgaca gaggcatctt ccgcattggt gatgccgcaa atcttgactt tgagtcccat
     2401 agacatgaac taggcttgcg ccgctgaacg cagaccgcgg agttcttgaa ttttggtccg
     2461 gacatcgtcg gctctgatta gagattctcc gacgagcatg gcgtggactc ccatctcaag
     2521 aagctgttcg acatggccgc ggttctgtat gccgctttcg ctgacgatga tccggtccga
     2581 tggaatacgt ggagccagcc gtttggtaac ggtgagatcc gtcgaaaacg tggtgaggtc
     2641 tcggttgttg atcccgatca ttctggcgtt gggaatccat tcaaggacgg tttcgagttc
     2701 acgctcatgg tgaacttcta tcaagacgtc aagtaagagt tccccagcca aggcatgaaa
     2761 atctaccaac tgccgacgct cgagcgccgc gacgatcaac aggaccgcgt ccgcgccata
     2821 agcccgcgcc tcgtaaaact ggatctcttc aaccatgaat tccttgttta gcatcggtaa
     2881 tgcgactgac cgtttgatgg ctcggaggta gtcaaggctg ccttgaaaga aatccttgtc
     2941 ggtcaacacc gagagagccg aggcacccga ttcttcatag caccgggcga tggccacatg
     3001 gtcgaacttg tcttcaaatt cgggacgaag caggcccagg ctgggcgatg cttttttcac
     3061 ctcggcgatc agcgctggac taaccgagct tctggtggcc tcgagcgcga ctgagaatcc
     3121 caatgggcgg gagatgtcgc ggatttccgc tctaaggtcg gccaggtatc cgcggctctg
     3181 cttatgccgt acttcggcct tcttgtgttc gagaattctg gcgaggatca tgcgtgcgcg
     3241 cctttgctcg tgaaggacac tagacgggca agtttttctg cggccgcgcc cgagtcgatg
     3301 aggtcgccgg ccagccggta ggcctcctgt agcgttttcg cctttttccc cgccaccagc
     3361 gccggggcgg cgttcagaca gacgacatca cgcttggcgc cctttcttcc ctgcaagatc
     3421 tctcgcgtga tgcgggcatt atcttgtgga gatccgcctg ccagctcctt tggagatacc
     3481 cggttcagac cgaattcatg aggagtcagg acatagttcg agaggacgcc ggctttggct
     3541 tcagacacaa tggtgcgctc ggtaagggta atctcgtcga gcccgtccat gccgtgaacg
     3601 acaaaacaat gttgagaccc aagatgcatg agcaccttcc ccagcagttc cgtcaggcgg
     3661 ggctcgtaca cgccgagcac ttgaacggtc gcgccggcag gattggtcaa cggaccaagc
     3721 agattgagca tcgtacggat gcccatctcc tgcctgggcc ctgcgcaatg tcgcatggcg
     3781 ccgtgataga gaggcgcaaa caagaatccg attcccacct cattgatgca ctcggagacg
     3841 agatccggag aaagactgat attcacgccc agctcggaca gtacatcggc actgcctgac
     3901 ttggatgaca ccgaccggtt accgtgtttc gccaccgtca gtccggcccc cgccacgacg
     3961 aaggcagtcg tcgtcgagat attgaaggta tgacggccgt cacctccggt gccgcacgta
     4021 tcgatcacga gcggagcgcc gacggcaata cgtactgcac gagcccgcat cgcgcggacc
     4081 gacccagcga tttcctcgac ggtctcccct ttcagacgaa ggcccatgag gtaagccgaa
     4141 atctgcgccg gagtggcgat cccctccatg atctggttca tgacctcttc cgcttctttc
     4201 tctgtcagcg aaagccggtc ggccaatttt gcgatggcgt ctctgatcat ggccatgcgc
     4261 gcggttacag cttgaggaaa ttgcggagca ggtccttccc cgccgctgta agaatggatt
     4321 ctggatggaa ttgcacacct tccacgccga gagatctatg gcgcatcccc atgatttctc
     4381 cttcggcggt ttccgcggag acctcaaagc aggccggaag gcttgagcga ttgacaatga
     4441 gcgaatggta tcgcgtggcg tcgaagggat tgggtaattg ccggaaaatc gtccgcccgt
     4501 cgtgctgtat gggagatgtt ttgccgtgca tcaagcgcac ggctctgacg acttctccgc
     4561 caaatgcgac tgccagcgac tggtgaccaa ggcaaacgcc cagaagcgg
//
LOCUS       DASTQY010000299         7404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_85972, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000299 DASTQY010000000
VERSION     DASTQY010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85972"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFR82_09845"
     CDS             <1..213
                     /locus_tag="VFR82_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501943.1"
                     /translation="RATSGRRQHDAILLAWVGSSHHRLSTWSKLAENGISQTSLTVTL
                     LNEHLLGLKRFSPSSFLQEPDPSRYV"
     gene            395..538
                     /locus_tag="VFR82_09850"
     CDS             395..538
                     /locus_tag="VFR82_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501944.1"
                     /translation="MPTVTAAMEEQGERPDQFRVIEEVIVARGLYEEAFGKRFRLGEE
                     AMK"
     gene            complement(772..1989)
                     /locus_tag="VFR82_09855"
     CDS             complement(772..1989)
                     /locus_tag="VFR82_09855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEU4501945.1"
                     /translation="MSITLKSERLETEKRQAVIVSAVRTPMGSFNGVFSSVPATKLGS
                     ISIAEALKRVHVLPEHVDDVLMGCVLSAGLGQAPARQASIGAGIPDSIGATTVNKVCG
                     SSIKTVMMASQTIALGEANIVVAGGMENMTRAPYLLEKARQGYRLGHAEMVDSVIKDG
                     LWDVYNNFHMGNAGELCAAKYRLTRKELDDYALESYRRAQHAIDAGAFKPEIAPVDVP
                     QRKGAPLTITDDEEPKRIDLRKLREFKPVFQENGLLTVGNSPSCNDGAATLVVMAHEE
                     AARRQLVPMARIVGYAGAALAPEWLSIAPAEAIKLVLKKTGLSLGDIDLFEINEAFSA
                     VSLAINRELGLDPAKVNVNGGAIALGHPIGATGARIMTTLLYAMEARGVRRGLASLCI
                     GGGEALAIIFERI"
     gene            complement(1952..3919)
                     /locus_tag="VFR82_09860"
     CDS             complement(1952..3919)
                     /locus_tag="VFR82_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU4501946.1"
                     /translation="MSKPDIFQGSVAIAETARDRASYTGFLAGLFESDVRWKLFTSVE
                     IPPMSSPALDFLNRFRTTLLPLDPERVDSEGELPEAILKEFAQLGAFGIKIPHQYGGL
                     EFTQAEYQKVATLCGSVDASLTVLLSAAQSIGVPEPLRLFGTDKQKAKYLPRLAQGEI
                     SGFALTERNVGCDISKVETYAVRVLEQGKTVGYRLTGEKFFITNSAKRDGEFLSSMLV
                     VIARIVDRPEEVNDSRSQKRYGAFIVETQWPGCSVTRLRFEGVRAIYNGIPTFRDVYV
                     PIENRLGGEEDGLRIALATLTVGRLTLPAACLGGLKQCLSLMRWWGQSRVQWNRPIGE
                     HNLIGEKLCRVAAYTLALEAVMAFCGAWANKKGDLRLESAAAKIIGSEWYWEAVNELF
                     QVRGGRGFMTAEAQRKTGEPSLPVMRMLRDARINLVWEGTSEILRVWMAREALSPYID
                     RGLAFLQGTWTDKLAAPLYYAHMSLRSCLPFLRPTPTLFGNEYSRWVRFIEASARRVT
                     RSTLMATLRHQQRLHNKQLLLQHLVNDSLLLFPMAATLWYANQPEMRAKPGIQDLVDY
                     FCQDMADRLCPPSSLAGRIRRHKKDSVVYRLAKMLMSGQYDWLEEGIIPVLRNDTVAR
                     NMNSSTPTDLSSDLSAGQSNVDHTQVRTPGD"
     gene            4146..5471
                     /locus_tag="VFR82_09865"
     CDS             4146..5471
                     /locus_tag="VFR82_09865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEU4501947.1"
                     /translation="MRNVVVVDGFRTPLCKEGTDFRETDAEVLGAWVVREIITRLHRW
                     NLPLTTIDCVLGSNVATPMHAVNPTRVAAVTGGLPATIPADTVAGKNCGSGVTALYYS
                     SLRIRSGDADTVLAIGMEAMSRIPLVYDRTVADLLLQYGKARTLRERTAGVLALIPKL
                     LNLKRYPPRVGLISGLTDPMCDLIMGLTAENISKDPALGITREDQDAFAVRSHRNAAR
                     AWKNGLFADEVVPMYVPERSAYVGRDNGIREDANAQTFRDVKPVFDRHHGSVTPANAS
                     QITDAAAAMLLMEESKAKSLGLPILGYVKEYADFGYEPSCMGLAPVGAIATVLSRARL
                     TLKDFAVCEINEAFASVVLGIMRILDSSALMECKFGRYGWTERLGEIPHDDLNPHGGA
                     IALGHPVGVSGLRLAMTALFELKRRNAERALISMCVGGGQGVAMILERK"
     gene            5474..>7404
                     /locus_tag="VFR82_09870"
     CDS             5474..>7404
                     /locus_tag="VFR82_09870"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU4501948.1"
                     /translation="MSHEGSHSFSLPATLKHFKTAILDDGLLLVGFDYGGKSVNVLNV
                     ESMLEWQHIVQAVERSVAITGVVLMSVKEGNFCAGADLEQMHEAQRQGSFRSMEQLVE
                     IAHQLFDAMERSGKPFVSAVEGSCLGGGLELALACHRRIASTHPKTCFALPEVKLGIL
                     PGFGGTQRLPRLIGLPAALDLITSGRMVFPRQGLRMGLIDGMVSSIPSRMRTLEAIQK
                     ETLIQTAIAQARSLRSVPTRQRPVRLSQRLMGGPLVRVLLCGYARHQVKKRVRHFYPA
                     PLRAVDAMQRGMGMSVLNGSLIAEKPLLMELMASPVSRHLVSLFLAGEDVKRKAAAAH
                     NPVTRVGVLGAGLMGSQIAGQLAEKGYAVVLRDVASSILAGAMGRILKAQMTQVRKKI
                     ILPSELRYRLLRIAPTTTMKDVGPAPLVIEAVSEKLDVKRAVLAEFESEAGPDAIFAT
                     NTSSYTLADIALKAVHPQRCVGLHFFNPVAKMQLVEVVRAPFTSDQALTQACSIAKRL
                     GKFPLVVRDGPGFLVNRILSRYLAEAVILVGEGVPIRRIDSIAKDFGMAVDSGHPMGP
                     LELIDLIGLPVAIHVLASLAVLGSRIETSDALLRNLLGDSKTPVPFWKSGVENRQALE
                     VIADYRRVYPSEGGPLTDE"
BASE COUNT         1577 a         2029 c         2154 g         1644 t
ORIGIN      
        1 cgagcgacca gcggacgacg ccaacatgat gcgatactgc ttgcatgggt cggatcttcc
       61 catcatcggt tgagcacgtg gtcaaaactg gcagagaatg ggatcagtca gacaagtctt
      121 acagtcactt tgttgaacga acacctcctt ggattgaaac gattcagtcc cagtagtttt
      181 ctgcaagagc cggacccatc acgttatgtt taagtgcttc gccatccagg ataatttggt
      241 gatgggcttt tttgaaggac gttgtgttcc tcattaatgg acaaatgttc tcttacaggg
      301 aattgtggag ctaattgcgc cgaggccggc tataacggtc tcgctcagaa ctgtagtcgc
      361 tttgttgatg gggactgtag cgggatgcgt gaaggtgccg acggttactg cggctatgga
      421 agagcagggc gaaaggccgg atcagttccg agttatcgaa gaggtcattg ttgcgagggg
      481 cttatacgag gaggcctttg gcaagcggtt ccggttaggg gaggaggcga tgaaatagga
      541 agcgccctag gttcaagaag gcccaacacg aaacctctag ctcatctgca ttcactccac
      601 gatcagcctg ctctattttt tcctgcggtt caggttagct gaggtaggag tgcccagggt
      661 gcctgttatc aggcacattc aacgcagaat ccgatcgata agttgtccgc ccagcgcact
      721 tatgcgacag cgtgggattt gacgaagcgg gaagtcccat tccatccatc gttatatccg
      781 ctcgaatatg atcgccaacg cttcgccacc accgatacac aggctggcca atcctcgccg
      841 gacaccacgg gcctccattg cgtacagcaa cgtcgtcatg atgcgggcac cggtagcccc
      901 gatcggatgg cccagcgcga tggctccgcc gttcacgttg actttcgcgg gatcaagccc
      961 cagttcacgg ttgatagcga gagagacggc agaaaaggct tcgttgattt caaaaaggtc
     1021 gatgtcgcct agcgaaaggc cggttttctt gagcacgagt ttgatcgcct ccgccggtgc
     1081 gatggacaac cactccggtg ccagagcggc tccagcgtat cccacgatcc gcgccatcgg
     1141 aacgagctgt cgtcgtgctg cctcctcgtg cgccatcact accagcgttg ctgccccatc
     1201 attacaggaa ggggagttgc ccacggtgag caggccgttt tcctgaaata ccggcttgaa
     1261 ctcgcgcagt ttcctgaggt cgattcgttt gggctcttca tcatcggtga tggtcagagg
     1321 cgcgcccttc cgttgcggca cgtcgaccgg cgcgatctca ggcttgaaag caccggcgtc
     1381 aatcgcgtgt tgcgcgcggc ggtagctttc caatgcataa tcgtccagtt cctttctggt
     1441 aaggcggtac ttcgcggcgc acagttcgcc ggcgttgccc atatgaaagt tattgtagac
     1501 atcccacaga ccgtctttga tcacgctgtc caccatctcc gcatgaccca accggtatcc
     1561 ttgtcgtgcc ttctctaaga gataaggcgc tcgggtcatg ttttccatcc cgcctgccac
     1621 gacgatgttt gcctcgccaa gcgcgatggt ctgggaggcc atcatcaccg tcttaatgct
     1681 ggatccgcac accttgttga ctgtcgtggc gccaattgaa tcgggaattc cagctccgat
     1741 tgaggcttgc ctggcaggcg cctgacccag gcctgcactg aggacacaac ccatcagcac
     1801 atcatcgacg tgttcaggca gcacatgaac gcgcttgagg gcttccgcaa tcgagatact
     1861 gcccagcttg gttgcaggaa cggaactgaa cacaccgttg aagctgccca tcggcgttcg
     1921 cacggcgctg acgatgaccg cctgtctctt ctcagtctcc aggcgttctg acttgagtgt
     1981 gatcgacatt cgactgtcct gccgaaagat cggaagaaag atcggtaggg gtggatgaat
     2041 tcatgttacg agccaccgtg tcatttctca atacaggaat gatcccctct tcaagccagt
     2101 catattgacc gctcatgagc atcttcgcta ggcgatacac gacggaatct ttcttgtgcc
     2161 gccggattct tccggccaga gaggagggtg gacagagacg atccgccata tcctgacaaa
     2221 aataatcgac gaggtcttga atgcctggct tcgcccgcat ctccggctga ttggcatacc
     2281 agagggtggc ggccatggga aagagcaaga ggctgtcatt caccagatgt tgcaacagaa
     2341 gctgcttatt atggagacgt tgctgatgac ggagcgtcgc catgagtgtc gacctggtaa
     2401 cacggcgtgc agaggcttcg ataaaacgaa cccaccggct atactcgttt ccgaataaag
     2461 tgggtgtagg acggaggaat ggaaggcacg agcgcagaga catgtgcgca taatagagcg
     2521 gagcggcgag cttgtcggtc catgttcctt gtagaaaggc gaggccacgg tcgatataag
     2581 gagagagggc ttcgcgcgcc atccagacac gcagaatctc gctcgttcct tcccacacga
     2641 gattgatgcg agcgtcgcgc agcatgcgca taaccgggag agaaggctcg ccggtcttcc
     2701 gctgggcctc agcggtcata aatcctcgtc ctccgcgaac ttgaaacagc tcattgaccg
     2761 cttcccaata ccattcgctc ccgatgattt tggcggctgc cgactccagc cgaagatcgc
     2821 ccttcttgtt cgcccaggct ccgcagaagg ccatcacggc ttccagagcc agggtataag
     2881 ccgcgactcg gcagagtttt tcccctatca aattgtgttc accgatcggc ctgttccatt
     2941 ggacacggct ctgcccccac cagcgcatga gggacaggca ttgtttgaga ccgcctaaac
     3001 aggccgcggg caacgtcagc cgccccaccg tgagtgtggc aagggcgatc ctcagaccgt
     3061 cttcttcacc cccgagccgg ttttcaatcg ggacgtagac gtcacgaaat gtaggaatac
     3121 cattgtaaat cgctctgact ccctcgaatc ggaggcgggt gacagaacat cccggccatt
     3181 gtgtttccac aataaatgcg ccatagcgct tctgagaccg tgaatcgttc acctcctcgg
     3241 ggcgatctac aatccgcgca atgacgacca gcatggatga caaaaactca ccatcccgtt
     3301 tggcggagtt tgtaatgaaa aatttttctc cagtgagtcg gtagccgacg gttttcccct
     3361 gctccagaac acggaccgca taggtttcca ccttcgaaat gtcgcagccc acgttgcgtt
     3421 cggtcagtgc aaaaccggat atctctccct gcgccaatcg tggaagatac ttagctttct
     3481 gcttgtccgt tccgaagagg cgcaacggct caggaacacc gatcgattgg gcggccgaca
     3541 ggagcaccgt caacgacgcg tcgacgcttc cgcaaagggt ggcgactttc tgatattcgg
     3601 cttgggtaaa ttccagtcct ccatattggt ggggaatctt gatgccaaac gcgccgagtt
     3661 gggcgaactc ctttaagatc gcttcgggca attccccttc actgtcaacg cgttcggggt
     3721 ccagggggag cagggtggtc cggaatcggt tgagaaaatc aagggctggc gaactcatcg
     3781 gaggaatttc gacggaggta aagagcttcc aacgaacgtc ggattcgaag agtccagcga
     3841 gaaaaccggt ataggaggcc cgatcgcgag cggtttcagc gatcgccaca gaaccctgaa
     3901 atatgtccgg tttactcatg tgtagcgggt cctgttgagg actttcgaga ccagagccgc
     3961 cgtatccctc ctactttacc actgttttcc ggcgacagtg tgtaggcagg aggggttatt
     4021 cggtccctct aatgtaggac ccttaagaaa agcatggaaa acgccaatcc cgtggtacga
     4081 tatgggtagt cgggccgatg gcatcggcag tgatttcttc cgaatttgtg gatggagatt
     4141 gcacgatgcg caacgtggtc gtcgtcgacg ggtttagaac cccgctgtgt aaggaaggaa
     4201 cagactttcg cgagacggat gcggaagtac tgggtgcctg ggtcgtacgc gaaattatca
     4261 cccgtctcca ccgctggaat ctccctctca ccaccatcga ttgcgtgctc ggcagtaacg
     4321 ttgcaacgcc catgcatgcg gtgaacccga cccgcgtagc ggcggtgacc ggcggtcttc
     4381 ccgctacgat ccctgccgat acggtcgccg ggaaaaattg cggctcaggt gtgacggcac
     4441 tctactactc cagcctgcgt atcagaagcg gggatgccga tacggtgctc gcgattggca
     4501 tggaggcgat gagtcgaata cccctcgtgt atgaccggac tgtcgccgac ctgctcctcc
     4561 aatatgggaa agccagaacg cttcgcgaac gaaccgccgg cgtcctagct ctgatcccaa
     4621 agctgctcaa tctgaagcgc tatccgccac gagtcggatt gatctccggc ctcacggatc
     4681 cgatgtgcga cctcattatg gggctcacgg cggaaaatat ttcaaaggat cccgcccttg
     4741 gcattacccg ggaggaccag gatgcatttg cggtccgttc gcacaggaat gccgcccgtg
     4801 catggaagaa tggactgttc gccgacgagg tcgtgcccat gtatgtccca gagcgaagtg
     4861 cctatgttgg gcgggataat ggcattcgtg aggatgctaa tgcgcaaacg tttcgtgacg
     4921 tgaagccggt attcgacagg catcacggtt cggtcacgcc ggccaacgct tcgcaaatca
     4981 cggatgcggc tgccgccatg ctcctgatgg aggagagtaa ggccaaatcg ctcggcttgc
     5041 cgattctggg atatgtgaaa gagtatgccg attttggcta tgaaccttcc tgtatgggac
     5101 tggcccccgt gggggccatc gccaccgtat tatcccgcgc tcggttgacc ctcaaagatt
     5161 ttgcagtgtg tgaaatcaac gaggccttcg cttccgtggt gctggggatt atgcgaattc
     5221 ttgactcgtc cgctctaatg gaatgcaagt tcggccgata tgggtggact gagcggttgg
     5281 gcgaaatccc gcatgatgat ctcaaccctc acggcggtgc catcgctctc ggccatccgg
     5341 tcggggtatc aggattgcgc cttgcgatga ccgctctctt cgaactcaaa cgtcgaaatg
     5401 ctgagcgtgc cttgatcagc atgtgcgtcg gagggggaca gggtgtagcc atgatcctgg
     5461 agaggaagta ggtatgtcgc acgaaggctc tcattcattc tccctgccgg ccactctcaa
     5521 acacttcaag actgcaatcc ttgacgatgg acttttgctg gtcggatttg attacggcgg
     5581 gaaatccgtc aatgtgctga acgtcgagag catgttggag tggcagcaca tcgtgcaggc
     5641 agtcgagcga tcggtcgcca tcaccggcgt ggtgttgatg agtgtgaaag aagggaactt
     5701 ctgtgccggg gctgatctgg aacagatgca cgaggcccag cggcaggggt ccttccgctc
     5761 catggaacaa ctcgtagaga tcgcacatca actcttcgat gccatggaaa ggtcgggaaa
     5821 accatttgta tccgcggtag agggctcttg tctggggggt ggactcgaat tggcactggc
     5881 gtgtcataga cgcatcgcca gcactcaccc gaaaacctgt ttcgctttgc cggaagtcaa
     5941 actgggcata ctcccggggt tcggcggaac tcaacggctt ccgcgtttga ttggtctgcc
     6001 ggcagcgttg gacctgatta catcggggag aatggtgttt ccccggcaag ggctacggat
     6061 gggtcttatt gatgggatgg tcagctccat tccttctcgc atgagaacac tggaggccat
     6121 ccagaaagaa accctgattc agaccgcgat tgcgcaagcg cgttcgctcc gctccgttcc
     6181 gacccgacag cgccctgtga ggctaagcca gagactcatg ggtggtcctc tcgttcgtgt
     6241 gttgctctgc ggttatgcca gacatcaagt gaagaagcgc gttcggcatt tctatccggc
     6301 tccgcttcga gccgtcgacg ccatgcaacg cgggatggga atgagtgttc taaatggatc
     6361 gctgatcgcc gagaagccac tcctcatgga gctcatggcg agcccggttt cccggcatct
     6421 ggtcagcctg tttcttgccg gtgaagatgt gaaacgaaag gcggccgcgg ctcacaatcc
     6481 ggtgacccgc gtcggcgtgc tcggagcagg attgatgggc tcgcaaatcg cggggcaact
     6541 cgccgagaag gggtatgcgg tcgtattacg cgatgtcgcg tcgagcatcc ttgccggagc
     6601 gatggggcga attctgaaag ctcaaatgac ccaggtgcgc aagaaaatca ttctgccgtc
     6661 tgagctgcgc tatcggttgc tgcgaatcgc ccccacgaca acgatgaagg acgtaggtcc
     6721 agcgcctctg gtgattgagg cggtatctga gaagcttgac gtgaagcgag ccgtgcttgc
     6781 cgagtttgaa tcggaagcag ggcccgatgc gatattcgcc acgaacacgt cctcgtacac
     6841 attggcagac attgccctta aggcggtcca tcctcaacgg tgcgtggggc ttcacttctt
     6901 caaccccgtt gccaagatgc agttggtcga agttgtgcgg gctcccttta catcggacca
     6961 agccctgacc caggcgtgta gtatcgccaa gcgtctgggc aagtttcccc tcgtcgtacg
     7021 cgacgggcct ggatttctgg tcaacaggat cctgtcacga tatctggcgg aggctgtcat
     7081 tttggtcggg gagggcgtcc cgatccggcg catcgacagc attgccaagg acttcggcat
     7141 ggcagtggac agcggacacc ctatgggtcc actggaactc attgatttga tcggtctacc
     7201 agtcgcgata catgtgttgg cgtccctcgc ggtgttgggt tcgcgaatcg aaacgagtga
     7261 cgccctgttg cggaacttgc ttggagactc caagacacct gtgccatttt ggaagtcggg
     7321 agtggagaat agacaagcgc tcgaggtgat cgccgactat cgtcgggtct atccttcaga
     7381 aggagggccg cttaccgacg aaat
//
LOCUS       DASTQY010000300         3377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1724212, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000300 DASTQY010000000
VERSION     DASTQY010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724212"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VFR82_09875"
     CDS             <1..922
                     /locus_tag="VFR82_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU4501949.1"
                     /translation="TAQAYWSLQNNGVMERMTKADSADLALLQQVFHPSDFTPASEVA
                     FIHALKAALVAKAELTILHVSPKHELEWMEFPGVRSTLERWGLLPMNSSQRDVLKLGI
                     NIKKVQAKHDDPVESVTSYLSTHNTDLIVLATDQHKSKVQWLSQSIAGPIARKSRQMT
                     LFIPKGVEGFVSAGDGSISLSSILIPVAATPAAQPAVQAAARMVTRLECATGVFTLVH
                     VGEQADMPQVQLPDVPGWRWNTTVRPGDVVDVISETASTVSAGLIVLTTEGRNGFLDA
                     LRGSYSERILQKAPCPLLAIPASSFIASVV"
     gene            963..1736
                     /locus_tag="VFR82_09880"
     CDS             963..1736
                     /locus_tag="VFR82_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501950.1"
                     /translation="MMRQNALLGLAFILVCMTGCSSSIYGWQVRTSSTAFSPSFDPGT
                     LGQESVGVFGALTMPGLRGNEVGLDFMLGEVLHRVAPHIQIVSQQQTISKINQQGLAA
                     EYAQMRADAEQSHILNRDPLKKLGMAIGARYVFQPRLAAFTQTMYDRWTFPGFGVVVS
                     QTRQSNLRMSVQLWDAKTGELLWASMAEGTMQSEALAKDPVYLEDAVRVTLGSIVADF
                     LNRKTASTYTPLNKIVDELIQIPMPEAKSDSVKPDPPSQ"
     gene            1783..2211
                     /locus_tag="VFR82_09885"
     CDS             1783..2211
                     /locus_tag="VFR82_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501951.1"
                     /translation="MRTAAQWSVTSLMICLVISYGCQTVEHAEQPQWVQVPIADLSNV
                     EGKWDGLMRRVPPERRDDWLTVTIASNGRYQFSSLRTIGVFSGHGEFSVNEGKLQSRS
                     ERGTVEAVLYEAGSQRMLKAKGRAADGTEYTAEVKPVKVR"
     gene            2289..>3377
                     /locus_tag="VFR82_09890"
     CDS             2289..>3377
                     /locus_tag="VFR82_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU4501952.1"
                     /translation="MLKFLTFDQRAALRMTLVLIAVAVGACSINPVSNLPEVTLLTVE
                     QEKKLGGEEAKKVEQEMGLLDDAALNSYADALGQRLAKESPRQDIPYQFHVVDMVEPN
                     AFALPGGYVYVTRGLLALVNTEDELAGVVGHEIGHVAARHTVQRISRQGPFALITNLV
                     SGVTGFFVPVVGNIVGGVGNFAQSLVFSPYSRSQESQADEVGQEIAAKAGWDPLGLAV
                     FLDTLGREEVLLSDGPRKPSFFDSHPATPDRVKHTTKHAKDLKPASREPISASRDAFL
                     ARLDGLVVGQRAANGIVQGQTYRHPDLNFFIQFPPSWNVDNTPVQLASAPKDGGKAVV
                     VRAVAASNDPLDGARALEKASKSSLPKTQ"
BASE COUNT          731 a          905 c         1010 g          731 t
ORIGIN      
        1 gacagcccag gcctattgga gtctgcagaa taacggggta atggagcgta tgaccaaagc
       61 cgattccgct gatcttgctc tgttgcaaca agtatttcat ccttcagatt ttacgccggc
      121 gagcgaggtc gcctttattc acgcgctgaa agcggctctg gtcgccaaag cggagctcac
      181 cattctgcat gtctcaccca agcatgaatt ggagtggatg gagttccccg gtgtccgatc
      241 gacgcttgag cgctggggac ttctcccaat gaacagttct cagcgtgatg tgctcaagct
      301 aggcatcaac atcaagaagg ttcaggccaa gcacgatgat cctgtggaat cggtcacttc
      361 ctatttgagc acacacaata cggatctgat tgttctcgcg accgaccaac acaaaagcaa
      421 ggtccagtgg ctgagccagt caattgccgg tcctatcgca cgcaagtcgc gtcagatgac
      481 gctgttcatc cctaaaggcg tcgaaggatt tgtttcggcc ggggatgggt ccatctcgct
      541 atcgagtatt ctgattccgg tagcggcaac acctgcggcg cagccggcgg tccaggccgc
      601 tgctcggatg gtaaccaggc tcgagtgtgc gactggtgtt tttacgttag tccatgtggg
      661 agagcaggcc gatatgcctc aagtccagct cccggacgtg ccaggctggc gttggaacac
      721 taccgttcgg ccaggcgacg tcgtcgatgt catcagcgag acagcctcta cggtttccgc
      781 cggtctcatt gtgttgacga ccgagggccg aaacgggttt ctcgatgcgc tccgtgggag
      841 ttatagcgag cgaattttac aaaaggctcc ctgtccgcta ttggccatac ctgcgagcag
      901 cttcatcgcg tcggtcgtgt aggttgatat tcgtcgcgac tttgaagcat cggcggctac
      961 agatgatgcg gcagaatgcc ctgttgggtt tggctttcat tcttgtatgc atgacaggct
     1021 gctcgagttc gatttacggg tggcaggtgc ggacgagctc cacggcattc tcgccgtcgt
     1081 ttgatcctgg gacgctcgga caagaatcgg tcggcgtatt cggggcgctg accatgcccg
     1141 gactccgcgg gaatgaggtc gggctggact tcatgctcgg tgaagtgctg catcgagtgg
     1201 caccccatat acagatagtc agtcagcagc agaccatctc gaagatcaat cagcaaggtc
     1261 tggctgcaga atacgcacag atgagggcag atgcggagca gagccatatt ctcaatcgag
     1321 accctttaaa gaagctgggc atggccatcg gcgcgaggta tgtgtttcag ccgcggttag
     1381 ccgcctttac gcagaccatg tacgacagat ggacgttccc aggttttgga gtggtcgtct
     1441 ctcaaacgcg gcagagtaat ttgcgaatgt cggtccaact gtgggatgcg aagaccggtg
     1501 aactgctgtg ggcctcgatg gctgaaggaa ccatgcagag cgaggctctc gcgaaggatc
     1561 ctgtctacct tgaagatgcg gttcgagtga ccttgggcag catcgtcgcg gacttcttga
     1621 acaggaagac cgcgtcaacg tacacgccgc tgaacaagat cgtcgacgaa ctgatacaga
     1681 ttccgatgcc cgaagccaaa tccgactctg tgaaacctga tccgccgagc cagtaggatg
     1741 tcgttggtta aaccgcaccc atcgggtcaa acaggagcgc gtatgcgaac agctgctcaa
     1801 tggtcggtga catcgctcat gatctgcctc gtgatcagct acggctgcca aacggtggaa
     1861 catgcagagc agccgcaatg ggtgcaagtg cccatcgccg acttgtccaa cgtcgaggga
     1921 aagtgggacg gtctcatgcg gcgtgtgccg cctgaacgaa gagatgactg gctgacggtg
     1981 accattgcgt ccaatgggag atatcaattc agtagcctgc ggacaatcgg cgtcttcagt
     2041 ggtcatggcg agttctctgt gaatgagggt aaattgcagt cgcgctccga acgggggacc
     2101 gtcgaagcgg tactctatga agccgggtcg cagcgtatgc tgaaggccaa gggccgcgcg
     2161 gctgacggaa cggaatacac ggcagaagtg aagccggtca aagtgagatg aagggacgat
     2221 tcgaagctca aggcgaaacg tcgttgagcg gttccatctc ataacaccga cactatggag
     2281 atgatcgtat gctgaagttc ctcacttttg atcaacgagc tgcgctcaga atgactcttg
     2341 tgttgatcgc ggtggcagtg ggtgcatgct ctatcaatcc tgtcagcaat cttccagagg
     2401 tgactctcct cacggttgaa caagagaaaa aattgggcgg ggaggaagcg aagaaggttg
     2461 agcaggagat gggcttgctg gatgatgccg cattgaatag ttatgccgac gcgcttggtc
     2521 agcggctagc taaggagtcg ccacgccagg acatccccta tcagtttcat gttgtggata
     2581 tggtcgaacc gaacgccttt gctctcccag gcggatacgt atacgtcacg cgtggcttgc
     2641 tggccctggt gaatacagag gatgagttgg ccggggtggt cggccacgag atcggccacg
     2701 tggctgcccg acatactgtt cagagaattt cccgccaggg accgtttgcc ctcatcacca
     2761 atcttgtctc cggcgtcacc ggcttctttg ttccggtagt cggtaatatc gtgggtggtg
     2821 tcggcaattt tgcccaaagc ctcgtatttt ctccatacag ccggtcgcaa gaaagccagg
     2881 ccgacgaagt cgggcaggaa attgcggcca aggctggctg ggatcccctg ggactggccg
     2941 tatttttaga cacgctcgga cgcgaagaag tgctcttgag cgacggtccg cgcaagccca
     3001 gcttctttga ttcccacccc gcgacgcccg atcgggttaa acacaccacc aagcatgcga
     3061 aggaccttaa gcccgcctcc cgcgagccga tctcggcgtc ccgcgatgcg tttctggccc
     3121 ggctcgacgg tctcgtggtc ggacagcgtg ccgcgaacgg cattgtacaa gggcagacct
     3181 accgtcaccc tgatctgaat ttcttcatcc agttcccgcc atcctggaat gtggataaca
     3241 cgcccgttca attagcctcg gcaccgaaag atggaggcaa agcggtcgtg gtccgtgccg
     3301 tcgccgcgag taacgatcct cttgatggcg cacgtgcctt ggagaaggcg agcaagtcct
     3361 cgctccccaa gacgcag
//
LOCUS       DASTQY010000301         3877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1663523, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000301 DASTQY010000000
VERSION     DASTQY010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663523"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFR82_09895"
     CDS             complement(<1..373)
                     /locus_tag="VFR82_09895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4501953.1"
                     /translation="MKAVAIIPTYNERENIPTLIQRLLALPLDLDLFFIDDGSPDHTG
                     QLLDSMQARLPQLRVCHRCKKLGIGTAYRQAFHMLLREPYDRYIAMDADLSHPPESIP
                     SLLEATHHADLAIGSRYVADGG"
     gene            complement(657..1991)
                     /locus_tag="VFR82_09900"
     CDS             complement(657..1991)
                     /locus_tag="VFR82_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEU4501954.1"
                     /translation="MGRPKEKLPSEHVVLHDQRRAPRRTVDLRVNNGSRELETALLIS
                     QALSRHCTLEDLIVQTLDTALEAIKADSGSILIADASAQQLVLYRSVGEKPVLPGSAV
                     PWNHGIAGAVFQSAQHAIIVDADKDPSLPERIDKSTGLVIKNLVIVPLKRWQGEPIGV
                     IEILNKRDRPIDEDDVAVLTMISSLATPAIEEARLFEAKKLAEVAQMAGEMSHDIKNL
                     LMPVLCGAELLKDELEELFDKWSIRKTPESEKSRTMCHEVLAMVEEDVRRISDRVREL
                     ADCVKGMTSQPRFASCRVVGVVEAVFKTLDLMAKKKGVALKSTGLDDLPALEADEQRL
                     YNAFYNLVNNAISEVPPGGSVTILGAVAKTANVLDICVTDTGRGMPPEIRDSLFTAHA
                     ISRKAGGTGLGTKIVKDVVDAHKGHINVESTVGVGTTFRLILPLRQVKPGRS"
     gene            complement(2031..2435)
                     /locus_tag="VFR82_09905"
     CDS             complement(2031..2435)
                     /locus_tag="VFR82_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4501955.1"
                     /translation="MGLGRILVVDDEAHIRTTVRMALTKAGYDVVEAEDGEKGIQSIK
                     SGDNPLLVDMILCDMQMPKIDGVGAISYFLQQFPSVPVVVMTGHPDINTATKLMKQGV
                     KDYLVKPIEKEKLLAVVEDAVKHHGRKDRFHV"
     gene            complement(2706..3113)
                     /locus_tag="VFR82_09910"
     CDS             complement(2706..3113)
                     /locus_tag="VFR82_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501956.1"
                     /translation="MIRHIVVLGVIAIFVPSLSPASDQLDSSVYQCPGPSGDTLYTNQ
                     KRPGCEVMNLPELTVAPNRDTAILTYNARPYGLRSFPSDWFDYAGSVGSLRNRLTQGG
                     LYGMQDWLDYDAPVGSMRNSPANWPSPYLLYVW"
     gene            3628..>3877
                     /locus_tag="VFR82_09915"
     CDS             3628..>3877
                     /locus_tag="VFR82_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU4501957.1"
                     /translation="MGSKLYVGGLPYSATEQQLSDLFGAHGTVASARIISDKFTGQSR
                     GFGFVEMSSDSEAKAAITALNGSDMGGRSLTVNEAKPME"
BASE COUNT          885 a         1028 c         1061 g          903 t
ORIGIN      
        1 agccaccatc tgcaacatac cggctcccaa tggcgaggtc tgcatgatgt gtcgcttcga
       61 gcagacttgg aatggattcc ggtggatgcg acaaatcagc atccatggcg atgtaccggt
      121 cgtaaggttc gcgcaagagc atgtggaagg cttggcgata ggctgtccca attccgagtt
      181 tcttgcatcg atgacaaaca cgcagctgcg gcaatctcgc ctgcatcgaa tcgagcagct
      241 gcccggtgtg gtccggagac ccatcatcaa tgaagaacag gtcgagatcg agaggaagtg
      301 ccaataaacg ctgaatcagc gtgggaatat tctcacgctc attgtaggtg ggaatgatgg
      361 cgacggcttt caccgaagtc cttctaagcc tgcctaagaa aggaaacggg ccgtcaagcg
      421 accagaaatg gcccatgacc gtgtttcgat cagtttcgat caaaactgag cgtattctac
      481 tttttcgcgt gggcggggtc tatcgcggtg tgaggtgcga tgcacggtga tgtaggggag
      541 tcgaggaacg agccagcgtg cgatgcgtgg gaaatggctc ctgaaggttg ttcgattcct
      601 tgaatatctg aggacacgaa agggcgggag ggaaggaggc gagactatca agcttgctag
      661 gatcgtccgg gcttcacctg tctgagcggc aagattagac ggaaagttgt accgacccct
      721 acagtactct cgacgttgat gtggccttta tgggcatcga ccacatcttt aacgatcttg
      781 gtcccaagtc cggttccgcc ggcttttcta ctgatggcat gagcggtaaa gaggctatca
      841 cggatttctg gaggcatacc acggcctgta tcagtcacac aaatatcgag cacgttagcc
      901 gttttcgcta ccgctccgag tattgtcaca gagccgccgg gtggaacctc cgatatggca
      961 ttgttgacca ggttgtagaa ggcgttatac aaccgttgct cgtctgcttc taaggcgggc
     1021 agatcatcaa ggcccgtgct tttcagagcg actccttttt tcttcgccat caaatccagc
     1081 gttttgaaaa ccgcctcgac cacgccgaca acccgacacg acgcaaatct aggctgggaa
     1141 gtcatgccct tcacacagtc cgcgagctct cgaacccgat ccgagattct tcggacatcc
     1201 tcttccacca tcgcgagcac ttcgtggcac attgtacggc ttttttccga ctcaggggtt
     1261 ttccgaatgg accacttgtc gaacaactcc tcgagttcat ctttgagtaa ctcagctcca
     1321 cacaggaccg gcatgagcaa attcttgata tcatggctca tctccccggc catctgagct
     1381 acctcggcaa gtttcttggc ctcaaatagt ctcgcctcct cgatggcagg agtcgcaagc
     1441 gaagaaatca tggtcagaac ggcgacatca tcttcatcga tggggcgatc gcgcttattg
     1501 aggatttcaa tcaccccgat cggttcgccc tgccaccgct tcagcggcac gataaccaaa
     1561 tttttgatca ccagccctgt ggacttgtcg attctttcag ggagggaagg atctttgtcg
     1621 gcatccacga tgatggcgtg ttgggcggat tgaaaaacag cacccgcaat cccgtgattc
     1681 cacgggactg cagaaccagg gaggaccggt ttctcaccaa cggagcgata aagaacgagc
     1741 tgttgagctg acgcatcggc gatcagaatt gacccactgt cggctttaat cgcctctaaa
     1801 gcggtgtcca atgtctgcac aatcaagtct tcgagcgtac agtgacgtga aagtgcttga
     1861 gagatgagga gcgcggtttc taattctcta ctgccgttgt taacacgaag atcgaccgtg
     1921 cgtcgcgggg cgcgacgctg gtcatgaaga acaacgtgct ccgaaggcag tttctcctta
     1981 gggcgcccca tgccatccct ttcggcggcc tcggtccagt gcgggagaaa ctatacgtgg
     2041 aacctgtctt tgcgcccgtg gtgcttgact gcatcctcga cgacagcgag aagcttttcc
     2101 ttctcaatag gtttcacaag ataatccttg acgccctgct tcataagctt cgtggcggta
     2161 ttgatatccg gatggccggt catgacgacg acagggacag aaggaaattg ctgcagaaaa
     2221 taggagattg ccccgactcc gtctatcttt ggcatctgca tatcacacag aatcatgtct
     2281 accaggagcg gattgtcacc agacttgatc gactggattc ctttttcacc gtcctccgcc
     2341 tcgacgacgt catatccggc cttggtcaat gccattctga cggtcgttct gatatgcgcc
     2401 tcatcatcca ccacgagaat tctccccagt cccataatgc cctcctttac ggttgcggta
     2461 cacgacccaa aagtaatctc ggccatcctg caataaggct ttgtttttgg caagaaatag
     2521 tcgaattatg attggaaact taggcgagac ctgctcgtac agatgatatg actcacggga
     2581 gcaattgcgg catgaatcct attcggcctc cggttactaa gctgcaatac ccgacccgcc
     2641 acgtgccgcg aaatccagat gccccatgtt gttgtcgact caggattccc gttttcttcc
     2701 ccgtttcacc atacgtagag caagtaggga cttggccaat tggccggcga attacgcatc
     2761 gatcctaccg gcgcatcgta atcaagccaa tcctgcatgc cgtacagtcc tccctgtgtc
     2821 aggcggttcc gcaaggagcc gactgacccg gcatagtcga accagtcaga cgggaacgag
     2881 cgcaagccgt agggcctcgc gttgtatgta agtatggcgg tgtctcgatt gggcgcgacc
     2941 gtcagctctg gaaggttcat cacctcacaa cctggtcgct tctggttggt atacagggta
     3001 tcaccggaag gaccgggaca ttgatacacg ctggaatcga gctgatcgga cgcaggagag
     3061 agggagggga cgaagatcgc gatgacgccg agcaccacga tgtgtctgat catactgagc
     3121 tccgatcgtg agaggttcta tcttctagtc gattgacgac aagagtaggt tacatgaacg
     3181 acaagagcag ggtacatgac actttggtgg ggagagaaaa cagcaggcgc gggtcaaaga
     3241 ggtggttcct ggacctcgtt tcattctgac ttggttaaag aaaccaggac atacgcgcag
     3301 ggtctattgc ggtgcgatga acggggagac gaggaacgag ccgcccgccg cgcgccacaa
     3361 tggtccttta tgagtagaat gccccttttt cttgtcatca tgtgtaaggt tgtaaaccat
     3421 caccccctgt ggtacggttg ttttgtcgtc gtacgggcct cgcgacgaag ctggttcaat
     3481 gaggaaggtc cgtaacatcg acgttgcctt gtccgagaat cttgtcggaa gtcagaccta
     3541 agtcgccgca ttgtccgcgt cttcggcccg ctccttcccc cggcctcgtg ttcaagacaa
     3601 tgtcacattt ataaggagga accccacatg ggttcgaagc tttacgtcgg tgggttgccc
     3661 tattcggcga ccgaacagca attgagcgat ttgtttggtg cgcatggcac ggtggcttct
     3721 gcgagaatca tctcagataa attcacggga cagtcccgcg gatttggctt cgtggagatg
     3781 tcctcggatt ctgaggcgaa ggccgcgatc acggcgctca atggctcgga catgggtggc
     3841 cgctcgttaa cggtcaatga agcgaaaccg atggagc
//
LOCUS       DASTQY010000302         5541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_224471, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000302 DASTQY010000000
VERSION     DASTQY010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224471"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /gene="recR"
                     /locus_tag="VFR82_09920"
     CDS             complement(<1..495)
                     /gene="recR"
                     /locus_tag="VFR82_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEU4501958.1"
                     /translation="MAVDQQGLLSRLVRELVRLPGIGQKTAQRLAFHILKADRDDALR
                     LADAIRAVKDGLSFCRQCRNIAEGELCEYCLDPKRDQTRILVVEEPSTLYAVERAGAY
                     RGLYHVLLGALSPLDGVGPSDIRAEELVDRVKLGGIQEVILATNPTIEGEATAIYLTN
                     QLKPL"
     gene            complement(506..829)
                     /locus_tag="VFR82_09925"
     CDS             complement(506..829)
                     /locus_tag="VFR82_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU4501959.1"
                     /translation="MKNPLGNMANIMKQAQQMQAQMAKLQEEAATKTVTGSAGGGMVT
                     VTANGALEVVSVRIDPEALKDGDADMLQDVVLAATNEALKNARQLMADQMKSVTGGMN
                     IPGLF"
     gene            complement(826..2637)
                     /gene="dnaX"
                     /locus_tag="VFR82_09930"
     CDS             complement(826..2637)
                     /gene="dnaX"
                     /locus_tag="VFR82_09930"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU4501960.1"
                     /translation="MDYQVSARKYRPGTFDDVIGQPHVVQTLVNAVTSKRIAQAYLFS
                     GTRGVGKTTVARILAKALNCEQGPTGTPCSTCVNCTEITQGHSVDVIEIDGASNTSVD
                     DVREIRENVKFAPFHGTYRVYIIDEVHMLSNSAFNALLKTLEEPPPHVVFIFATTEIH
                     KIPATILSRCQHYNFRRIAKTEIIERLRHVADQDGITLEDRSLAALARASEGSMRDGL
                     SLLDQAVAFGGKSIVHADLEALLGAIPQEMVRSLIRAITSQDSAGALRTLSQLIEGGH
                     ELRAYCADVVEYVRHLLVVSVTPPQEWPALIEVSSDDLAQLAADKASLTAEHIQQIFT
                     LFLEAEDALRFSAHPRFVLETAAIKATRLLGPQSTTSDRPGRAVSAPGAPHPSVVRSD
                     KGSVTQVGGGSAHPARALQPQRAVTGTSSSSPGSGVPAASSMPRTDKTPATQPMRQQV
                     GQPPSTAPARPPQAIMLNWEQFQEEVGHTFPNIAPFLEMGRLVGIEGNVVTIGFAKQA
                     TVARGMLEKADNIQALASLCERMGGQPVRLRVIELTEADPPGPTMAQIRAAKEQEQKV
                     VLFEQAKAHPVVKQALEMFGAELADVRTVANQQEVQE"
     gene            complement(2769..2868)
                     /gene="ffs"
                     /locus_tag="VFR82_09935"
     ncRNA           complement(2769..2868)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFR82_09935"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3273..3362)
                     /locus_tag="VFR82_09940"
     tRNA            complement(3273..3362)
                     /locus_tag="VFR82_09940"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3326..3328),aa:Ser,seq:gga)
     gene            3471..5351
                     /locus_tag="VFR82_09945"
     CDS             3471..5351
                     /locus_tag="VFR82_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEU4501961.1"
                     /translation="MKILSVIVIVLALLIVALLSLPFLVDLASYQDHYKPLVEEALNR
                     KIQVQGIRLTIWPRIGARITGFTVMDDPSFSASPFASLSSLDVGVKLWPLLTKKIEVE
                     EITLRDPVITVIKSKAGVTNVSTIGPKAPVTADRREPDSPQPQGDPLEALALLAVDRL
                     SIDGGTVIYRDLSTAPETEYRIQDLVLGLKSVHLGETPTIHLSATTLPHNLPVTLDGS
                     FGPLVQALEIKQYQFTLAVGRMALALHGALLGGQLDAIVTAPSISTADVPVALPLIRP
                     VEIKDLRIVAKAPYPLKSGASPLALADVTDLTLAVVMGKSSLNVRGNAIGGHARVIVS
                     SPSVDTADLPVESGLKKPVEIRNVELNADLKGQEVRLSNLSLEVFNGQLKAQGGTSLG
                     TSTPPFNGKLTFQGVQIGQALAAVDPQSPLLVSGRAAADVAVAGRGFSWADLVRALEG
                     PGHVEIKDGKIEGVNLIGEAAALLKIAGLSFEQAKTTAFSIIESDFTIKQGRVNAQKI
                     HMDSHDFQATGHGTVGFDQTLNLAMNLNLSQALTQKIAGSSALVKAASQGGRLRLPLL
                     ITGTVQRPSFALDARELKGKVQEQVQEKVKDSIEGLMQGTTKPADLKKEGKELLKGIF
                     GR"
BASE COUNT         1171 a         1629 c         1533 g         1208 t
ORIGIN      
        1 gagcggcttc aactgattcg tcaggtaaat ggccgtggct tcgccctcga tcgtgggatt
       61 cgtcgccaag atcacttcct gaattcctcc gagtttgaca cggtcgacga gttcctcggc
      121 ccggatgtcc gatggcccga ctccatcgag aggagaaagt gcaccgagta ggacgtggta
      181 gagaccgcgg taggcgccag ctcgctcgac ggcgtacagc gtactgggtt cctcgaccac
      241 gaggatcctg gtttggtcgc gcttgggatc caagcaatac tcacatagct cgccttcggc
      301 gatgttccgg cattgacggc agaacgagag gccgtctttg acggcgcgaa tggcgtccgc
      361 caatcgcagc gcatcatctc gatccgcctt gaggatatga aaggccaagc gctgggcggt
      421 cttttggccg atgccaggga gacgaaccag ctctcgaact aggcgggaca gcaacccttg
      481 ttgatcaaca gccattggtg actcgttaga acaggccggg aatattcatg ccgccggtca
      541 ctgatttcat ttgatcggcc atcagctgac gcgcgttctt taaggcctca ttcgtggccg
      601 ccagcacgac atcctgtagc atatccgcat ctccatcttt cagggcctcg ggatcgatac
      661 ggacgctgac aacctccagc gcgccgttcg ctgtcaccgt gaccatgccg cctcccgccg
      721 agccggtcac cgttttggtc gcagcttcct cctggagctt agccatttgc gcctgcatct
      781 gctgcgcctg tttcatgatg ttcgccatat tgccgagagg atttttcatt cctgcacctc
      841 ttgttggttg gcgaccgtcc tgacatccgc aagttccgcc ccgaacattt ccaaggcctg
      901 cttgacgacc ggatgggcct tcgcctgttc aaagagtacg actttttgtt cctgctcttt
      961 cgctgctctg atctgggcca tcgtaggacc gggagggtct gcttcagtca gttcaatgac
     1021 acgaagccgg accggctggc cgcccatccg ctcgcataac gaggccagcg cctgaatatt
     1081 gtccgccttc tccaacatcc ctcgtgccac cgttgcttgc ttcgcaaatc cgattgtcac
     1141 gacattgccc tcgatcccaa ccaggcgtcc catttcgaga aagggtgcaa tatttggaaa
     1201 cgtatggccg acctcctctt gaaactgctc ccagttcagc ataatcgctt gcggagggcg
     1261 tgccggcgca gtcgaggggg gctgccccac ctgctgtctc atcggttgag tagccggtgt
     1321 tttatcggtg cgcggcatgg atgacgccgc cggaacgccg gagccgggag aagatgagga
     1381 ggttccagtc acagcgcgct ggggttggag tgcacgtgca ggatgggcgg agccgccgcc
     1441 aacttgtgtg accgatcctt tatcagaccg gacaacagat ggatgtggag caccaggagc
     1501 ggagaccgca cgtccaggcc ggtcacttgt tgtgctctga gggcccagca accgcgtcgc
     1561 ctttattgcg gccgtttcca gcacaaatct aggatgagca ctgaaccgca aggcgtcctc
     1621 cgcctcgaga aacagcgtaa agatctgctg aatgtgttcg gcggtcagcg acgccttgtc
     1681 cgccgcgagc tgtgcaagat cgtctgagga aacttcaatc agtgcgggcc attcttgcgg
     1741 gggcgtgacg gacacgacca gcagatgacg cacatactcc accacatcag cgcagtacgc
     1801 acgcagctcg tgcccacctt ctatcagttg agacaatgtc cgcagagctc ccgcgctgtc
     1861 ctgcgacgtg atcgcccgaa tcaaggatcg aaccatttcc tgcggaatgg cacccagcaa
     1921 agcttcgaga tcggcgtgga caatcgactt accaccaaaa gccacggctt gatccaggag
     1981 actcaaccca tccctcatac tcccttcgct ggctctggct aacgcagcca gactccggtc
     2041 ctccagcgtg atgccgtcct ggtcggctac atggcgtagc cgctcgatga tctccgtctt
     2101 ggcaatgcgg cgaaaattgt aatgctggca gcgggaaagg atcgtcgcgg ggatcttgtg
     2161 gatttccgtc gtggcgaaga tgaacacgac gtgaggaggc ggctcttcca gcgtcttgag
     2221 aagggcgttg aacgccgagt ttgacagcat gtggacttcg tcaataatat acacacgata
     2281 ggtgccgtga aacggggcga atttgacgtt ttcgcggatc tcacggacat catcgacgct
     2341 cgtattcgac gcgccgtcaa tttcgatcac atccacggaa tgaccctgcg tgatctcggt
     2401 gcaattgaca caagttgagc agggtgtccc cgtcggtcct tgctcacaat tcagcgcctt
     2461 ggcaagaatg cgtgcgacag tggtcttgcc cacgccgcgc gtccccgaaa acaaataggc
     2521 ctgagcgatc cgcttgctgg tcacggcgtt gacgagcgtt tggacgacat ggggctgacc
     2581 gatcacatcg tcaaacgttc ctggtcggta ttttctggcg gaaacttggt agtccataat
     2641 ggtgttcttt cacatgcagg atggtgaaga ggctatctaa cacatttgag catacagttt
     2701 cctgtacgtc gaggatggca catgtcggaa aacgatgtga gacgcctgtt tcaacatctt
     2761 aaaacaagcg gggccaggtg atcctgcggc acccaaaaca ttctgcttac cgttgcttcc
     2821 ttccggacct ggcggggttc acagggttct gttgcacagg gcccagcccc tagttcttgc
     2881 cgagagctga gtcaaagctc aatccagtac acctccggag caatcgcctc tgttcgaatc
     2941 ccactttcag catggaccac ttgaagtatt aatcccgtca cctttaactg acgccccgga
     3001 tgagcaagct cattagacct gagactgaac agcacttggg gtgtctatcc acccgtaaca
     3061 atcacgatag agcgctcgga gcgggcagtt cacacgaagt gtggtatggc ggagagggtg
     3121 ggcattcgaa gtcgcgctcg cctgacactg ctcgcgctcc tactgagggg gccagccccc
     3181 tcgtacgctc cccattgaag gggacacatc cccttcagaa cccctcttcg aatcccaccc
     3241 tttcagcacc gaagaactcg aggtgtcttg tatggcggag agggtgggat tcgaacccac
     3301 ggtcccgttg ccgagacgca tgctttccaa gcatgtcgat tcgtccactc tcgcacctct
     3361 ccgtcaaagc atcgaagagg gaagtttagc atgcactcct atgagcggca aggcgcgttg
     3421 accggctcac tcagctttct tatactgcgc cgagctgtca ggagacctgc atgaagatcc
     3481 tttcggtgat agtcatcgtc cttgccctcc tgatcgtcgc cttattgtcc cttccttttc
     3541 ttgtcgatct cgcctcctat caagaccact acaagcctct cgtcgaagaa gcgttgaacc
     3601 gcaaaatcca agtgcaaggg atcagactga cgatttggcc tcgcatcggc gcccggatta
     3661 ccggcttcac agtcatggac gatccgtctt ttagcgcgag cccgttcgcc tcgttgagct
     3721 cgctggatgt cggcgtgaag ttgtggccgc tcctcacaaa gaaaattgaa gttgaagaaa
     3781 tcaccttgcg cgatcccgtg atcaccgtga tcaagagcaa ggccggcgtc acgaacgtct
     3841 cgacgatcgg ccccaaagca cctgtgactg ccgaccgcag ggagcctgac tcgccacaac
     3901 cgcagggcga tccgttggaa gctctcgcac tcctggctgt cgaccgtctg tcgatcgacg
     3961 gaggcacggt aatctatcgc gacctctcga cagcgccgga aacggaatat cgaatccaag
     4021 atcttgtgct gggcctgaag tccgttcatc ttggcgagac gccgaccatt cacctcagcg
     4081 caaccactct cccgcacaat cttcccgtca cattggacgg cagctttggc ccgctggttc
     4141 aggcgcttga gatcaagcag taccagttta ctctcgcggt cggaagaatg gctctcgcat
     4201 tgcacggagc gttgcttgga gggcaattgg atgcgattgt gaccgctcca tccatcagca
     4261 ctgctgatgt cccggtagcg cttcctttga tcaggccggt cgaaattaaa gaccttcgta
     4321 ttgttgccaa agcgccgtac ccgctcaagt caggcgcctc gccactggca ctcgccgacg
     4381 tgacggacct gactctcgcg gtggttatgg gcaaatcctc gctgaacgtc agagggaatg
     4441 ccattggtgg ccacgccagg gtgatcgtct cgtcaccgtc cgtggatacg gccgaccttc
     4501 cggttgagag cggcctaaag aaacccgttg aaataagaaa tgtagagctg aacgctgatc
     4561 tgaagggcca agaggtacgg ctcagcaatc tctcactcga ggtcttcaac ggacagctta
     4621 aggcgcaggg cggcacgtcg ctggggactt ccacgccgcc cttcaacggc aagctgacct
     4681 ttcaaggtgt gcaaatagga caggcgcttg ccgcggtcga tccgcagagc ccgctcctgg
     4741 ttagcggaag agccgcggcc gacgtggctg tcgcaggacg cgggttttct tgggctgatc
     4801 tggtgagagc gctggaggga ccagggcatg tggaaatcaa agacggaaaa atcgagggcg
     4861 tgaatctcat cggtgaagcc gccgcattgc tgaaaatcgc cggcctttcg tttgagcagg
     4921 ccaagacgac ggcgttctct atcatcgaaa gcgacttcac gatcaaacaa ggtcgtgtta
     4981 acgcgcaaaa aatccacatg gacagtcatg actttcaggc gaccgggcat gggacagtcg
     5041 gcttcgatca gacgctcaat ctcgccatga atttgaatct ttcgcaagca ctgacccaaa
     5101 agatcgccgg ctcgtccgcg ctcgtgaagg cggcatcgca gggcggccgg ctgcgattgc
     5161 ccttgctcat caccgggact gttcaaaggc cttcattcgc actggatgcc cgagaactga
     5221 agggaaaagt ccaggaacag gttcaggaaa aagtcaaaga tagcatcgaa ggtctcatgc
     5281 aaggcacgac caaacccgcc gacctcaaga aagaagggaa agaactctta aaagggatct
     5341 tcggacgata gctgtgtttt cgtgtccgtc cttccactac cttattcgcc agcatctgca
     5401 cagccatctg tccttgcaac gatacacagc ctgttgttcc tgccgagaaa tcgctaagtc
     5461 ttttgtgttg gctatttgcc gccgctgcag cgtcttccgc taccgcacac cttatttcgt
     5521 gattgccaac ctctgagtat t
//
LOCUS       DASTQY010000303         3093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_244393, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000303 DASTQY010000000
VERSION     DASTQY010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244393"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            524..1096
                     /locus_tag="VFR82_09950"
     CDS             524..1096
                     /locus_tag="VFR82_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501962.1"
                     /translation="MNVTTVAKQEDWTRPAAMAIPQGGFPETEQGRYGPIFPKTPACY
                     GFSILAKIIPGREEVFYDYAKNIEKTVASQPDALAVLKLHYLRWILFPINGETYFMYQ
                     GIFDTDFDKYTEDAVALFSATGIDTVFENLEGFPLDWKTKPEEFVKFVREHQIPSFLE
                     YGEYPYVSADEIKKALKLKAAFSNMLDQMQ"
     gene            1121..2464
                     /locus_tag="VFR82_09955"
     CDS             1121..2464
                     /locus_tag="VFR82_09955"
                     /inference="COORDINATES: protein motif:HMM:NF044706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501963.1"
                     /translation="MSPGDPIAALEFDDIQHILLTRTPALTGRYEFLSFRSPSGGRAW
                     LKEILKTVQSAADVLDSVDEDKRWITVAFTWNGLKALGVDETSLATFPDEFQQGMPAR
                     AEMLGDTGANHPANWVGGLASADLHAIAILFARDEAERARCISEHQELLADCEGVEEL
                     SSLDLLAIPPFDTAHDHFGFRDRLSQPVIEGTDEVPTPGSGAPLKPGEFILGYPDENG
                     PPVDLPQPEILSRNGSYMAYRRLQEHVGSFRDFLRQHGETPEEQELVAAKLMGRWRSG
                     APLVLAPDKEDPVLGVDMQRNNDFNYKEMDPHGYAVPLGSHMRRMNPRDTAANINRRR
                     MIRRGATYGLPLPEDAPDDGTDRGIAAFIICASLIRQFEFAQNVWANDRNFHELGNER
                     DPIIGMQDGTLEFKIPKRPIRKTITGLPAFTTVRGGAYFFLPGLKALRWLADLKA"
     gene            2530..>3093
                     /locus_tag="VFR82_09960"
     CDS             2530..>3093
                     /locus_tag="VFR82_09960"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEU4501964.1"
                     /translation="MADHGAGPRATANPEIDITDLFAFPSPERPGHLVLIMNAYPFKH
                     IEATRLLFSDAIDYRIRVRPVRIAATGPGAAFAVGEQECFFSFTFAVPRQSGNQLTQT
                     GTCRGPLGEIAVRVGDEAGARGDGVHVFAGCRLDPFFVDQLFSGGLRVTRKIPKITGI
                     NSVEGQNVLSIVVEVEHAKLFGPDAGPL"
BASE COUNT          684 a          895 c          891 g          623 t
ORIGIN      
        1 tggactggcg aaagaagcag ctgaccggga aaaaaaatcc attcagtaat ggtcgtgacg
       61 aagcgcagcc tgctaccaag atggtcttga gctgatcctc gatgcagcct cagctggcaa
      121 atgtcgagac gcaagaacgt ccgcttttgg cacttagcgg acatccatcc cgaagccggg
      181 catatcgctc ttgggagtta agcggacatc cctgattgcc gctgtgatgt ccgctaatga
      241 cccaaagcgg acacggcgca gttcagcagc ggaagcgcct gatgacgctg aggtgtaagc
      301 tcgtgcaaca aatgtctttt ctacaaacgg ctctaaaatt gctagactaa gcgacgaagc
      361 ccctagcgga gggcaccgtg ggtggagtct cagtccagcg aacgaggctg acgacacgcg
      421 gacagactgc gctttgccat ttgctttatt gagttgtcac cggccgcggc cctccgggct
      481 gcgcaatgcc ttaggatcaa gtcttgtgta ggaggagacc acgatgaacg tgaccacagt
      541 cgcaaagcaa gaggattgga ccagaccggc ggcaatggcg attccccaag ggggcttccc
      601 tgagacagag cagggcagat acggcccaat ctttcctaag actcccgcgt gttatgggtt
      661 ctcgattctc gcgaagatca tcccgggtcg ggaggaggta ttctatgatt atgccaaaaa
      721 catcgagaag accgtagcgt ctcagcctga cgctctcgct gtgctcaagc tgcactacct
      781 gcgctggatc ctctttccga tcaacgggga gacttacttc atgtaccagg gcatcttcga
      841 taccgacttc gataaatata ctgaggatgc tgtggctctc ttctccgcaa ccggaattga
      901 taccgtgttc gaaaacctcg aaggttttcc cctggattgg aagaccaagc ccgaggagtt
      961 cgtgaagttc gtccgcgagc atcagatccc gagcttcctc gaatatgggg aataccccta
     1021 tgtgagtgcc gacgagatca agaaggccct aaagctaaag gccgctttct cgaacatgct
     1081 cgaccagatg caatgacctc ggaaagcggg aggcgcagac atgagcccag gggatccgat
     1141 tgcggcgctc gaattcgacg acattcagca catcctgctc acgcgtactc ccgcactcac
     1201 tggacgatat gagtttttgt cattccgcag cccttcgggc ggccgcgcgt ggctaaaaga
     1261 gattctcaaa acagtacaat cagcggctga tgttctggac tcggtcgatg aggacaagcg
     1321 ctggataact gtggcgttca cttggaacgg cctcaaggct ttaggagtgg acgaaacgtc
     1381 gctcgccaca ttcccagatg agtttcagca gggcatgccc gcacgcgcag aaatgctcgg
     1441 ggacactggc gctaatcatc ctgccaactg ggttggggga cttgcgagcg cggatcttca
     1501 cgccatcgcc atcttatttg cccgcgatga agccgagcgt gcgcgttgta tttcagagca
     1561 ccaggagctc ttagcggact gcgaaggcgt tgaggagctc tcatccctag acctcctagc
     1621 gatccctcca ttcgatactg ctcacgatca cttcggtttc cgcgaccggc tctcgcagcc
     1681 agtcatcgaa ggcaccgacg aggtgcccac gcccggctcc ggcgcgccac taaaaccagg
     1741 ggaattcatc ctcggctacc cggacgagaa cggaccgccc gtagacctac cgcaaccaga
     1801 aatcttgtct cgaaacggca gctacatggc ctatcgccgc ctgcaagagc atgtcggaag
     1861 ttttcgtgac tttctgcgcc agcatggcga gacgccagag gagcaggaac tcgtggcggc
     1921 aaaacttatg ggtcgatgga ggagcggtgc tccactcgtg ctggctccgg ataaggaaga
     1981 tcctgtgtta ggcgtcgaca tgcagcgaaa taacgacttc aactacaaag aaatggatcc
     2041 gcatggatat gcggtgcccc tcggctcgca catgcgtcgc atgaatcctc gcgacactgc
     2101 agcgaacata aaccggcgcc gcatgatccg ccgcggtgct acttatggtt tgcctcttcc
     2161 ggaagacgct cctgacgatg gtactgaccg aggcatagcg gctttcatca tctgtgcaag
     2221 cctaattcga cagttcgagt ttgcgcagaa cgtatgggcc aacgaccgca attttcatga
     2281 gctcggcaac gagcgcgatc cgatcatcgg catgcaggac ggcacgctcg aatttaagat
     2341 ccctaagcgg cccattcgca aaacgatcac cggcctgcct gccttcacta cggtgagggg
     2401 cggcgcgtac ttctttctgc ctgggctcaa ggcgctgcgc tggctggccg acttaaaggc
     2461 gtaaaataaa gcggggctcg tcgagttgtc agaccgacac gtcgcgtcca cgcatcgtag
     2521 gtgaaacaaa tggcggatca tggtgcagga ccgagggcta cagccaaccc cgagatcgac
     2581 atcaccgact tattcgcctt tccaagcccc gagcgtcccg gccacctggt gctgataatg
     2641 aacgcttacc cgttcaagca tatcgaagcc acacggctcc tgttctcgga cgccatcgac
     2701 taccggatcc gcgttcgccc ggtgcgcatc gcggctacgg ggccaggcgc cgcatttgcg
     2761 gtcggcgagc aggaatgctt cttcagcttc acgttcgccg ttccgaggca gagcggcaac
     2821 cagctgaccc agaccggcac ctgccgaggc ccgttgggcg agattgcggt tcgcgtgggc
     2881 gatgaagccg gcgcgcgggg tgacggcgtc catgtcttcg ctggttgccg gcttgacccg
     2941 ttctttgtcg accagctgtt ttcgggcggg ctcagagtga cacgcaagat ccccaagatc
     3001 acggggatca attcggtcga agggcagaac gtcctcagca ttgtcgtcga ggttgagcat
     3061 gcgaagctgt ttggacccga cgcggggccg ctg
//
LOCUS       DASTQY010000304         9230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1267326, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000304 DASTQY010000000
VERSION     DASTQY010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9230
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267326"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..190
                     /gene="rpsU"
                     /locus_tag="VFR82_09965"
     CDS             2..190
                     /gene="rpsU"
                     /locus_tag="VFR82_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU4501965.1"
                     /translation="MKVFNNNVEKALKVAKKKLAGEGLFRELKRRRYYEKPSVRKKAK
                     QREAQRRRQKWLSKRRPE"
     gene            245..904
                     /gene="nth"
                     /locus_tag="VFR82_09970"
     CDS             245..904
                     /gene="nth"
                     /locus_tag="VFR82_09970"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU4501966.1"
                     /translation="MRQDQIHEAIRILKREVRQWQEPVVGVIARTSARDPFRILISCL
                     LSLRTKDKTTSEASARLFALAYTPGRLLNLPRRTIERAIYPVGFYRTKAKAIHAICRR
                     LLDEYGGTVPDSIDELVTLPGVGRKTANLVVTVGYKKPGICVDIHVHRISNRWGYVKT
                     AKPEETEQALRVKLPKRYWIIFNDLLVPYGQNICQPVSPFCSRCKLAGFCDRVGVVRS
                     R"
     gene            complement(935..1357)
                     /locus_tag="VFR82_09975"
     CDS             complement(935..1357)
                     /locus_tag="VFR82_09975"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEU4501967.1"
                     /translation="MSLPSPKFVLFAHNATYDKLHQVATLGLTAAAMGKDVIVILLFW
                     TIKKLAEGKFDQIDFPPEYSASAETVGRLLKEKQVPTISQMFEEAKLVGKVRIIACSA
                     GLEYMGVDGEAVSKQVDEVMGLPAILSLTADAETKLFI"
     gene            complement(1360..1704)
                     /locus_tag="VFR82_09980"
     CDS             complement(1360..1704)
                     /locus_tag="VFR82_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501968.1"
                     /translation="MRATLLTSLLCWVLLWLIATVCLPPVLSAASDDLVIAISKKGLG
                     KEVVITQGPREWFMLVEVTPENTVIVRQEKERDTYLLDESETHDRPLAPTEVDAAIND
                     YINSVKARVPKQ"
     gene            1814..2245
                     /locus_tag="VFR82_09985"
     CDS             1814..2245
                     /locus_tag="VFR82_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEU4501969.1"
                     /translation="MDWLLSSVQQYVSTETLIWLTVLSIIFFVGTLIAIPFILVRLPA
                     DYFDVRVPRPWMQNHHPVLRVVGHIVKNALGAVFVFAGFLMLFLPGQGILTMLIGVSM
                     LDFPGKRKIEAKLIGQPTVLGVINGMRKRRGKPPLILAPDS"
     gene            2288..4942
                     /gene="polA"
                     /locus_tag="VFR82_09990"
     CDS             2288..4942
                     /gene="polA"
                     /locus_tag="VFR82_09990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU4501970.1"
                     /translation="MSEAAVSSRPTLYLIDGSAYVYRAFFALPPLNNSKGLQTNAVYG
                     FTTTLMKIIREHQPQGLAVAFDEPGPTLRHETYKAYKAQRPPMPEGMSGQIPYIHRIV
                     EAMNIPAVKLAGYEADDLIGTLACQAERAGYDVVIVTGDKDMFQLLAPHIRIYDPVKS
                     KWFGEAECRARFGVEPARVVEVMGLMGDSTDNIPGVRGIGEKTAIKLITEFGTIEELL
                     RRLDEVTPPRIKTLLVEQADSARLSRTLATIDTQSPVAFQADTYRVKSLHHDRFADLL
                     RELEFTTLLKNLQPAEGRVERKAVSVASLEDQASIRRFTHDLPPGVPLGLQCLLAGGS
                     GMQADLLGLALSVEGRTGFVPLKSDPLEHSLAALLRDSGRQKVIHDLKAALLACHRAG
                     LVLNGPYVDTMVADYLLNPNRRDHQLETIALEVLGERLETSKEKSEQSLFEVETASRE
                     DVAQSAAVLARLGPVMTQRIAEQGSGKLFAEVEMPLVPVLVEIERNGFLLDVEKLHAL
                     GKELDRELERMMDTIAGLAGGEFNINSPKQLATVLFEKLGLKPVRKTKTGYSTDEDTL
                     TQLALQHELPSQILNCRSMSKLKSTYVDALPQLVNPQTRRLHTSLNQTVAATGRLSST
                     EPNLQNIPVKGDFGLRIREAFVAPDGHALLCADYSQIEPRILAHLSQDQRLLAIFAQS
                     EDIHLATAMEIFSLPADQITKEMRRAAKTVVFGIVYGISPFGLSQNIGVPQAEAKKYI
                     ETFFERFPAVRALMDRNIAEGKVKGYTTTILGRRRPIPELQSGDPVQRSFGERMAVNS
                     PIQGSAADLIKVAMIRVHQALQEQLPQTKIILQVHDELIFEVPEANLEAAKQLVKTEM
                     EAVGQTLGLSVPLKVDLGVGKNWRAAHP"
     gene            5122..5988
                     /locus_tag="VFR82_09995"
     CDS             5122..5988
                     /locus_tag="VFR82_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501971.1"
                     /translation="MIHAVELDSQTSSFVRQLEEQRRIEKNLMVERVRGSKEGLESVF
                     HDIKERVDALERMTNSVSTSYIAPIGWSQLIFAGTDGLSAAPTIGFGDRDDSICKHDR
                     IRYLEDANYLRLSANPHYVERVLPNKSAHARAAANPSTWDNLGYVHVGYSTALVLPAS
                     LEPTTVSILSRVNTLFNWVEVNAVADDWYAHVAATARLWVAYDGEFRQTPPAQFVNLL
                     AMPKQVKTHQHIDSLAPSAALAMQLEVSPGSGTTLLMYETIELVATTPNAHAYIDGVF
                     AWEPVAVQLREG"
     gene            complement(6006..7271)
                     /locus_tag="VFR82_10000"
     CDS             complement(6006..7271)
                     /locus_tag="VFR82_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501972.1"
                     /translation="MPIENNFQHDELSRKHPGERLAAAEFTAGPPPDSPEWTAEMVRL
                     GSAMGRAGVRSVILVHGAIHGTDVFGTQRLDDAGGLKRGYSRGVSGLDALLAAMRQET
                     NGIPLLPGGLRPPLTNDEATKQLIDHHVGDAGNFTETALGMLQRALNKASTSIRCVRL
                     LWSSEHHHLGRAVAAVRVLEELRMHCELHQLASGDRILIQAHGQAGLVLALVSNLLCP
                     SAITGRPRLLAILTSYAEQTNQTDLAAVINRLAPLLSAGSLLNGTSLDIVTFGQPVRY
                     GWDPSGIGKLLHVVNHRNLRTDAKTWLAKMELPQITMEMPIAWGGDYVQEHAVAGSDA
                     LPETEQAKAANKAAWEMVEPFDGFERWLECARRAIRCPSEGRCLLVDYKDSTGSTNPR
                     DHYYGHAAYTRRDAMLFNTGHIVDTFYQS"
     gene            complement(7321..7860)
                     /locus_tag="VFR82_10005"
     CDS             complement(7321..7860)
                     /locus_tag="VFR82_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501973.1"
                     /translation="MAALALGTPSPSWMPPTLVSDSGSVRSHGEHSFQQLQQQQTGFI
                     TSNNELPGDSRNEAGFSPDEFVIGKGQGDMAKGVLVCQRVSELSARTDLAKQIRVLVK
                     EHMIDRVRERSGRSVDQDIELTREEIVQEYLQGVKIVDRQIDVEKNICSATAVMPKNK
                     ILQPGPLIDRQESTPVITR"
     gene            complement(7888..>9230)
                     /locus_tag="VFR82_10010"
     CDS             complement(7888..>9230)
                     /locus_tag="VFR82_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501974.1"
                     /translation="FAPRRSFLLLFSLPCFTILLLCLGEIASAEIRIVVSQGEYRMGD
                     RDTKEDAMRLATEAAKRNALEQVATYLESITVVEGIDVTKDEITTYTAGLVVVHDQQT
                     NLSLDGDSVIITVDLTAQIDTEEVAQAILTLRENEDARQQLAALKQDIDDLRKELELA
                     TQSLAVAVSVEQAQQASLQRQELLDRVQSNAMVSQAWTDWVMVGPVTYPSQWVGITPP
                     HMLLTMARELYPGSPHVPAAQRVIVTSTPAAATQTILPTTHLIPIQPGARTTPLTLNE
                     ISHTTPTMPVHLGNQPGGITQAPTRVGTRTFTDMRRLTPLSPPASGNAPAGGQTRPPA
                     SPSVQSLQQFLQAPTAMAPSRGSRDIQQGSRSIQSLRQFLQPPTNVPPTGQPPVTRAV
                     PTAPNHIQSPLFVPRVPYHIAPRFSEGPGRYGRGGRGGYGLGRAGGGRGGGKGR"
BASE COUNT         1959 a         2598 c         2669 g         2004 t
ORIGIN      
        1 aattaaagtc ttcaataaca acgtcgagaa agcgctcaaa gttgcgaaga aaaagcttgc
       61 cggagaagga ctctttcgag aactgaaacg tcggcggtat tacgaaaagc cgagtgtccg
      121 caagaaagct aaacagcggg aagcacagcg ccgccgtcaa aagtggctgt ccaagcgccg
      181 ccccgaatag tcctccgtct tatcagtcgc catccgccac caccagtctt gtttcgcttc
      241 tctcatgcgt caggaccaga tccatgaggc gatccggatc ctcaaacggg aggtccggca
      301 gtggcaggaa ccggtggttg gtgtgattgc cagaacctct gctcgggatc ccttccgcat
      361 tctcatttcc tgtttgttga gcttgagaac aaaagacaag acgacgagtg aagccagcgc
      421 ccggctcttc gctttggcat acacacccgg caggctcctg aacctgccgc ggagaaccat
      481 tgagcgcgca atttatccgg tgggatttta ccgaacgaag gcaaaagcta ttcatgcaat
      541 ttgccgtcgg ctcttggatg agtatggggg cacggtgccc gattcgatcg atgagctcgt
      601 cactctgcca ggcgtcggcc gtaagacggc caacctggtg gtcacggtgg gctacaagaa
      661 gccaggtatt tgcgtggaca tccacgttca ccgcatcagc aaccgctggg gatatgtgaa
      721 gaccgcgaag cccgaagaga ctgagcaggc gttacgcgtt aagctgccca agcggtattg
      781 gatcatcttc aacgacttgc ttgtgcccta tgggcaaaac atttgccagc cggtgtcccc
      841 cttctgcagt cgttgcaaac tggcggggtt ctgcgatcgc gttggagtgg tcaggtcgag
      901 gtaggactag agcgagcggc tcgtcacaag gcaatcaaat aaacagcttc gtttctgcgt
      961 ctgccgtcag tgagagaatc gcaggcaagc ccatcacttc gtccacctgc ttcgaaacgg
     1021 cctcgccgtc cactcccatg tattccagac cggcactgca agcgatgatg cgaacctttc
     1081 ccaccagctt ggcctcctcg aacatctgcg aaatcgtcgg cacttgcttc tctttgagca
     1141 aacgtcccac ggtttccgcc gaagcggaat attccggagg aaagtcgatc tgatcaaact
     1201 tgccttccgc cagctttttg atagtccaga acagcaggat gacgatgacg tccttcccca
     1261 tcgcggctgc ggtcagcccg agcgtcgcca cctggtgaag cttatcgtag gtggcgttat
     1321 gggcaaaaag aacgaacttc ggtgaaggga ggctcatgct cactgtttgg gaaccctcgc
     1381 tttgacgctg ttgatataat cattgatcgc cgcgtcgact tccgtagggg ccagcggccg
     1441 gtcatgtgtt tcactctcat cgaggagata ggtgtcccgc tctttttcct ggcggacgat
     1501 caccgtattc tcaggtgtga cctccaccaa catgaaccat tcgcgagggc cctgggtgat
     1561 cactacttcc ttacctaaac ccttcttgct gatcgcaatg acgagatcat cggacgccgc
     1621 agacaggact gggggaaggc atacggtcgc aatcaaccaa aggagcaccc aacacagtag
     1681 cgatgtgagc agggtcgcgc gcatggcgcg attatagctg cggccataat cccagtaaac
     1741 ctgccgtcga gttggtgatc tgcgtccccg taggtcgcat gttcgccagg tcgcgtgtta
     1801 cgatgccggc ccgatggatt ggctcctttc gtcagtgcag caatatgtgt ccactgagac
     1861 cttgatttgg cttactgtcc tgtcgattat ctttttcgtc ggtacattga ttgcgattcc
     1921 cttcattctg gtgcgcttgc ccgccgacta tttcgacgtg cgggtcccgc gtccttggat
     1981 gcagaatcac catccggtgc ttcgggttgt cggccacatt gtcaagaatg ctctgggcgc
     2041 ggtgtttgta tttgccggat tcttgatgtt gtttttgccc ggccagggca tcctgacgat
     2101 gctcatcgga gtctccatgc ttgactttcc tgggaagcgt aaaattgaag cgaaactgat
     2161 cggtcaaccg accgtattag gtgtcatcaa cggcatgcgg aagaggcgcg ggaaaccgcc
     2221 gctcatcctc gcaccggact cgtaatccga aacgggcctg aatccctatt ccgactcccc
     2281 gatatttatg tcagaagctg ctgtctccag ccgcccgaca ctctatttga ttgacgggag
     2341 cgcgtacgtc tatcgggcct tttttgcctt gccgccgctg aacaattcca aaggcctgca
     2401 aaccaatgcg gtctacggct tcaccacgac gttgatgaag atcatccgcg aacaccagcc
     2461 gcagggcctt gcagtcgcct tcgacgagcc cgggcccacc ttgcgacatg agacctacaa
     2521 ggcttacaag gcgcaacggc cgcccatgcc ggaagggatg agtgggcaaa tcccgtatat
     2581 tcatcgcatc gtcgaggcga tgaatattcc cgccgtcaag ctggccggct atgaagcaga
     2641 tgacctcata ggcaccttgg cctgccaggc agagcgggcg gggtacgacg tggtcattgt
     2701 gacgggagac aaggacatgt ttcaactttt ggcgccgcac atacggatct acgatccggt
     2761 gaagagcaaa tggttcggag aagcggaatg tcgtgctcgt ttcggtgtcg aaccggctcg
     2821 ggtcgtggaa gtcatgggcc tgatgggaga cagcaccgac aacattcccg gagtcagagg
     2881 gatcggcgag aaaaccgcca tcaaattgat cacggagttc ggcacgatcg aggaactgtt
     2941 gcgacgtctg gatgaggtca ccccgcctcg catcaaaacc ctcttggttg aacaggccga
     3001 tagtgcccga ctcagccgga cacttgccac catcgacaca caaagtccgg tcgcgtttca
     3061 ggccgacacg tatcgggtca aatctctgca tcatgaccgc ttcgccgacc tcctgcgcga
     3121 gttggaattt actaccctac tcaagaatct tcagccggcg gaaggccggg tcgaacggaa
     3181 agcggtgagc gtcgcgtctc ttgaagacca agcttcgatc cgtcgcttca cccacgatct
     3241 gcctccaggc gttccgctgg gccttcagtg cctgcttgca ggcggatccg gcatgcaagc
     3301 ggacttactg ggactcgcgc tttcagttga gggcaggaca gggttcgtac cgctcaaatc
     3361 cgacccactt gagcactcct tggcggcgct cttgcgcgac agcggtcggc agaaagtcat
     3421 tcatgatctc aaggccgcac tactggcttg tcaccgggcg ggactcgttc tgaacggtcc
     3481 ttacgtcgac accatggttg ccgattacct cctcaatccc aaccgccgcg accatcaact
     3541 tgaaacaatc gctctggagg tgttgggtga acgactggag acctcaaagg agaagtccga
     3601 gcagtccctg ttcgaagtgg aaacggcgtc gcgggaagac gtggcccaat ccgctgcagt
     3661 cctggcacgt ctcggccccg tcatgactca acggatagca gaacagggaa gcgggaagct
     3721 atttgccgag gtcgaaatgc cgctcgttcc ggtcttagtc gagatcgagc gaaacgggtt
     3781 cttgttggac gtcgaaaagc tgcatgcgct cgggaaagaa ttggatcgag aactcgaacg
     3841 catgatggac accattgccg gcttggccgg aggagaattc aacatcaatt cgccgaagca
     3901 actagccacc gtgctcttcg aaaagctcgg actcaaacct gtgcgaaaaa caaaaacggg
     3961 gtattcgacg gatgaagaca cgctgacaca actggccctt cagcacgaac tgccttccca
     4021 gatcctgaat tgccgcagca tgagtaagtt gaaatcaacg tacgtcgatg cgctgccgca
     4081 attggtcaac ccgcaaacac gaagactaca tacctccctg aatcaaaccg ttgcagcaac
     4141 cgggaggttg tcgtcgactg aaccgaatct ccagaacatt ccggtcaagg gtgacttcgg
     4201 gttgcggatc cgggaggcgt tcgtggcgcc ggacggacac gcattgctct gcgccgatta
     4261 cagccagatc gagccgcgaa ttctagcgca tttgtcgcag gatcaacggc tgctggccat
     4321 ttttgcgcag agcgaggata tccacttggc cacggcgatg gagatattca gtctgccggc
     4381 cgatcagatt acgaaagaga tgcggcgagc agcgaagacc gtcgtctttg gtattgtcta
     4441 cggaatcagc cccttcggct tgtcgcaaaa tatcggggtg ccacaagcag aggctaagaa
     4501 atatatagaa acgttctttg aacggtttcc tgcggtccgt gcgctcatgg atcggaacat
     4561 tgccgaaggc aaagtcaaag ggtacacgac aacgattctt ggccgtcgcc gaccaattcc
     4621 ggaattacaa agcggcgatc cggtccagcg cagcttcggc gagcgcatgg cggtgaacag
     4681 tccgatccag ggctctgcgg cggacctcat caaagtggcc atgatccgcg tccaccaggc
     4741 attgcaggaa caactgccgc agacaaagat catcttgcaa gtccatgacg aactgatctt
     4801 tgaggtgccc gaagccaacc tggaggctgc gaagcaactc gtcaaaacgg agatggaagc
     4861 ggtggggcag acgctcggcc tttccgtccc gctcaaggtc gatctcggag tcggcaaaaa
     4921 ttggcgcgcc gcgcatcctt agtgatctgc tctgtccgac ctcgcttctg acaccgacgt
     4981 tcaaacctgt gccgtatgtc cgaaatcccc ttcaggtcgc cctgtgcttt cactgctttg
     5041 cgcggagtta ggggtggcac tcctcttgcc atggctcttt cgtttcctcg tagagtgacc
     5101 tccagaaaga aagggactca catgatccat gctgtggaat tggacagcca gacctcttcg
     5161 tttgtgcgac aactggaaga gcagcggcga attgaaaaaa acctgatggt ggaacgcgtc
     5221 cggggctcaa aggaaggcct tgaatctgtc tttcacgaca tcaaagaacg ggtcgatgcg
     5281 cttgaacgga tgaccaactc ggtgagcacc agctatatcg cacccattgg ttggagccag
     5341 ttgatcttcg cagggacgga cggcttgtct gcagccccaa ccatcgggtt cggtgatcgc
     5401 gatgacagca tctgtaaaca cgatcggatt cggtatctcg aagacgcaaa ttatttgcgg
     5461 ctgagtgcca accctcatta cgtggagcgc gtgctaccca ataagtcggc gcatgcgcgc
     5521 gccgccgcta atccatcgac atgggacaac cttggctacg ttcacgtcgg ctattcgact
     5581 gcgctcgtcc taccggcctc tttggagccg acgacggtct cgatcctgtc gcgggtgaac
     5641 acgctgttca attgggtcga ggtcaatgcc gtggcggacg attggtatgc tcatgtggcg
     5701 gcgaccgctc gcctgtgggt ggcatatgat ggggaatttc gtcagacgcc gccagcgcaa
     5761 tttgtcaacc ttttggcgat gccgaagcag gtcaagactc accaacatat cgattcgctg
     5821 gcgccaagcg cagcgctggc catgcagttg gaggtgtcac ctgggagcgg gacgacactc
     5881 ctcatgtatg agaccattga attggtcgcg accactccca acgcccatgc ctacattgac
     5941 ggagtgtttg cctgggaacc ggtggccgtt cagttgcggg aagggtgacc agggtctgag
     6001 gaagactacg actgatagaa cgtatcgacg atgtgtccgg tgttgaacag catggcatcg
     6061 cgccttgtgt aggccgcgtg gccgtaataa tgatcgcggg gattcgtgga tccggttgag
     6121 tctttgtagt ccacaagcaa acagcggcct tcgctggggc aacggattgc gcgccgcgca
     6181 cattccagcc agcgctcgaa gccatcgaat ggctccacca tttcccaggc cgccttattg
     6241 gcggccttcg cctgctcggt ttccggaagc gcgtcgctgc cggccacggc atgttcctgc
     6301 acatagtcgc ccccccatgc gatcggcatc tccatcgtga tctgaggcaa ctccatcttt
     6361 gcaagccagg tcttggcgtc tgtacggaga ttgcgatggt tcacaacatg gagcagcttc
     6421 ccgattcccg aagggtccca tccatagcgc accggctgcc caaaggtcac aatgtccaat
     6481 gaagtgccgt tcagcagcga acccgcagat aggagcggag cgagtctgtt gatcaccgct
     6541 gccaagtccg tttggttggt ctgttcggcg taagatgtga ggatggcaag cagccttggt
     6601 ctcccggtaa tagcgctcgg gcagaggagg tttgatacca gtgcgaggac gaggccggcc
     6661 tgaccatgcg cttgaatgag gatgcgatct ccagacgcca gctgatgcaa ctcgcaatgc
     6721 atgcggagct cttcgagtac acgcactgcg gccacagccc tcccgaggtg atgatgctcg
     6781 gacgaccaca agaggcgcac gcatcttatg gatgtggacg ctttgttcag cgcgcgttgc
     6841 agcatgccaa gtgccgtctc tgtaaagttc ccggcatcgc cgacgtggtg gtcgatgagc
     6901 tgcttcgttg cttcgtcgtt ggtgagcggt ggtcggagcc cgccgggcaa aagcgggatc
     6961 ccattcgttt cttggcgcat ggcggcgagc agcgcatcga gtcccgaaac cccacgtgag
     7021 tagcctcgtt tgagtccgcc cgcgtcatcg aggcgctggg tcccgaagac atcggtgccg
     7081 tggattgcac cgtgtacgag tatgaccgag cgcacgccgg cacgacccat ggccgacccc
     7141 agtctgacca tttccgccgt ccactccggg gagtccggtg gcggaccggc cgtaaattcc
     7201 gcggcggcta accgctctcc cggatgtttt ctggacagct cgtcatgctg aaagttgttc
     7261 tcaatcggca tcgtatgatt tcatactcga tctcggtggc gcactgcaat ggaaggatgg
     7321 ttagcgggtg atgacaggtg tcgattcctg acggtcgatg aggggcccag gttgcaagat
     7381 tttatttttg ggcatcaccg cggtcgcaga gcagatgttc ttttctacat caatttgacg
     7441 atcgacgatc ttcacgcctt gaaggtattc ctgcacgatc tcttcacgag tcaattcgat
     7501 gtcctgatcg acgcttcttc ccgaccgttc ccggacacgg tcgatcatgt gctctttgac
     7561 taagacccta atttgtttgg ccagatcagt acgcgcagac aattcggaca cccgctggca
     7621 caccaggaca cctttcgcca tatcgccttg gcctttgcct atcacaaatt catctggtga
     7681 aaagccggct tcattccggc tgtcgccagg taattcattg ttggaggtaa tgaagccagt
     7741 ttgctgttgc tgaagttgct ggaacgagtg ctcgccgtgg ctcctgacgc tacctgaatc
     7801 tgagacgaga gtcggaggca tccacgatgg cgaaggggtt ccaagcgcca gagctgccac
     7861 caggagctgt aaaccgttcg tcaccgatta tcttcccttt ccgcccccac gaccgccgcc
     7921 tgctcggccg agtccgtacc caccacgtcc ccctcgtccg taacggcccg gaccttccga
     7981 aaaccgagga gcaatatggt agggcacacg cgggacgaac aggggcgatt gaatgtgatt
     8041 aggcgcggtc ggcactgcac gcgtgactgg cggctgtcct gtcggaggga cgttggtcgg
     8101 tggctggagg aactgtcgca gcgactgaat ggatcgggag ccctgctgaa tgtctcgtga
     8161 tcctcttgat ggcgccatgg ctgtcggggc ttgtaaaaat tgctggagtg actgaacgga
     8221 cggcgaggcg ggtggacgag tctgcccgcc tgccggtgca ttgccagaag cgggcgggga
     8281 gagcggggtc aaccgccgca tgtctgtgaa ggtccgggtg ccgactcgag tcggggcttg
     8341 agtaatgccg cctggctgat tgccgagatg cacgggcatc gttggtgtcg tatgggaaat
     8401 ctcgttgagt gtcaaaggcg tcgttcgagc gcccggctga atcgggatca aatgagtggt
     8461 tgggagaatg gtttgcgtgg ctgcggccgg cgtgcttgtc acgatgaccc gctgggcggc
     8521 cgggacatga ggactgcccg gatacagttc ccgcgccatg gtcagcagca tgtgtggagg
     8581 tgttatgccg acccactgag acggataggt caccggtccc accatgaccc agtcagtcca
     8641 tgcttgcgaa accattgcat tcgactgcac tcggtccaag agttcttgcc gctggagact
     8701 ggcttgctga gcctgctcga ccgagacggc gacggctagc gattgagtgg ccaattccag
     8761 ttccttacgc aagtcatcga tgtcttgttt caatgccgcg agttgctgtc gcgcgtcctc
     8821 attttcacga agggtaagga tcgcttgagc cacctcttca gtgtcaattt gggctgtaag
     8881 atcaacggtg atgatcacgg agtcaccgtc gagcgacagg ttcgtctgtt gatcgtgaac
     8941 cacaaccagt ccggcagtgt atgttgtgat ctcatccttg gtgacgtcga taccctccac
     9001 gaccgtaatg ctttccagat aggtcgccac ctgttccaac gcatttcgtt tggcggcttc
     9061 cgttgccaga cgcatggcat cttccttcgt atcgcggtca cccattcgat attcaccctg
     9121 ggacacgacg atacggatct cggcggatgc tatttcaccg aggcacaata gaaggatcgt
     9181 gaagcaggga agcgagaaga gaagaaggaa tgaccttcgc ggcgcaaatg
//
LOCUS       DASTQY010000305         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1635230, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000305 DASTQY010000000
VERSION     DASTQY010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635230"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /gene="rpsD"
                     /locus_tag="VFR82_10015"
     CDS             <1..539
                     /gene="rpsD"
                     /locus_tag="VFR82_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU4501975.1"
                     /translation="CAIERRSYPPGQHGQARPRISDYSTQLREKQKLRRIYGLQERQF
                     RGVFERAERQSGVTGEALLRLLECRLDNVAYRLGFGASRKEARQLVGHGHVTVNGRKN
                     NIPGSQVRPGDVVAVRERSRSLASIQTALESVEGRGIPEWLELDRAGFKGTVRALPTK
                     DQITLPVNEQMVVELYSR"
     gene            618..1616
                     /locus_tag="VFR82_10020"
     CDS             618..1616
                     /locus_tag="VFR82_10020"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU4501976.1"
                     /translation="MIKAMKDFQIPMRVEVDKETASATFGRFTTEAYERGFGTTVGNA
                     LRRVLLSSLTGAAVTTVKIEGVLHEFSTIPGVTEDVTSIILNVKGLRLASHTDKPKTI
                     RLKKKGPGEAKGSDIIHDADIAILTPNLHIATLDKDATLDMEMTVKHGRGYVPAERNK
                     EEGLPIGVIAIDSIFSPIKRVNFHVENARVGRMTDYDKLTLEIWTDGTISPSDALSTA
                     AGILRDHVDIFINPEARVEGRAELSGDDAQREINKNLFRSVNELELSVRAANCLKNAN
                     IKTIADLVQKSEAEMLKTKNFGKKSLNEIKEILTDMGLSLGVKLDAMASGSGSKNE"
     gene            1629..2045
                     /gene="rplQ"
                     /locus_tag="VFR82_10025"
     CDS             1629..2045
                     /gene="rplQ"
                     /locus_tag="VFR82_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU4501977.1"
                     /translation="MRHRKKGRQLGRQTKHRWALFRSLVTSLLEHERIETTEAKAKEI
                     RGFTDRMITLGKDGSLPARRRALSFLRSKDVVSKLFSDVAGRFKDRPGGYTRMVKTRR
                     RIGDAAELVAIELVARAEVSTSKKPGSTPAKTDKTE"
BASE COUNT          532 a          602 c          683 g          421 t
ORIGIN      
        1 agtgcgcgat tgagcgccgc agctatccgc ccggtcagca tgggcaggcg cggccgcgca
       61 tttcggacta cagcacgcag ttgcgtgaga agcagaaatt gcggcgcatt tatggccttc
      121 aagaaaggca gttccggggt gtcttcgagc gggccgagcg ccagtcgggc gtcaccggcg
      181 aggcgttgct gcgattgctc gaatgccgtc tggacaacgt ggcgtaccgc ctcggattcg
      241 gggcgtcccg caaggaggcg cgacagctcg tgggacacgg ccatgtgacc gtcaatggcc
      301 ggaagaacaa catcccaggg tcgcaagtgc gtccgggcga cgtggtagcg gtgcgggaac
      361 gcagccgcag cctggcgtcg attcaaaccg cgctggaatc ggttgaaggc cggggcattc
      421 ctgaatggtt ggaactggac agggccggtt tcaagggcac ggtgcgggca ttgccgacga
      481 aggaccagat cacgctcccg gttaacgaac agatggtcgt cgaattgtat tcgcggtaac
      541 ggcaacttca tacaacgccg gctttgtgca gagtgcatcg cagaaagtgc ggcggctgac
      601 aggatgaagg gggactcatg attaaagcga tgaaagattt tcaaatcccg atgcgggttg
      661 aagtggacaa ggaaaccgct tccgccacct ttgggagatt cactacggaa gcctacgagc
      721 gtggcttcgg cacgaccgtc ggcaatgccc tgcgccgtgt cctgctctca tccctcaccg
      781 gagcggcggt gacgaccgtg aagatcgaag gcgtgctgca cgagttttcg acgattcctg
      841 gtgtgacgga agacgtcacg tcaatcatcc tgaacgtcaa aggattacgg ttggcctcac
      901 acacggacaa gccgaaaacg atccgattga aaaagaaagg acccggtgaa gccaaggggt
      961 ccgatatcat tcatgatgcc gacatcgcca tcctgacacc caatcttcac attgcgacac
     1021 tcgacaagga tgccacgctc gacatggaaa tgacggtcaa gcatgggcgc ggctatgtac
     1081 cagcggaacg caacaaagaa gaagggttgc cgattggcgt tatcgccatc gattccattt
     1141 tttcgccgat caagcgcgtg aactttcacg ttgaaaatgc ccgtgtcggc cggatgaccg
     1201 actatgacaa actgaccctc gagatctgga cggacgggac catctcgccc agcgatgcct
     1261 tgtcgactgc cgccgggatc ttgcgtgacc atgtggacat cttcatcaat ccggaggcca
     1321 gagtggaagg ccgcgctgag ttgagcggcg acgacgccca gcgcgaaatc aacaagaatc
     1381 tcttccgaag cgtcaatgag ttggagttgt ccgtgcgcgc agcgaactgc ttaaagaacg
     1441 ccaacatcaa gaccatcgcg gatctcgtgc agaaatcaga ggccgagatg ctgaagacca
     1501 agaattttgg caagaagtcg ctgaatgaaa tcaaagaaat tttgacggat atgggattga
     1561 gcctcggggt caagctggat gcaatggcct caggcagcgg ttctaaaaac gaatagaaag
     1621 agggcaccgt gcgtcaccga aaaaaaggca gacagctggg gcgccagact aaacaccgct
     1681 gggccttatt cagaagcctc gtcacctcgc tactcgaaca tgagcggatt gagacgactg
     1741 aagccaaggc caaggagatt cgtggtttta cagaccgcat gatcacgctt ggaaaggacg
     1801 gctccctgcc ggcccgccgg cgtgcgttga gcttcctgcg cagcaaggac gtggtctcca
     1861 agctttttag cgatgtggcc ggacggttca aggatcgtcc cggcggatac accagaatgg
     1921 taaagaccag gcggcggatc ggcgatgcgg ctgaactagt cgcgattgaa ctcgtggcac
     1981 gcgccgaagt ctcgacctca aaaaagcccg ggtcgactcc agccaagaca gacaaaacgg
     2041 agtaggtctc gcctcgagac ctactcgcga cgcgcggctt gaccgccgtc gtgtgatcaa
     2101 tcagaacctc gcctcagcgc tgcatgtttt ttcgttgcta acggtgcgca gagtttactt
     2161 gccatcagtc ggatgctatt atcgtgccag gatttttctc cttgaggcac cggacggaaa
     2221 cattgtgtgg cagcgttg
//
LOCUS       DASTQY010000306         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1478373, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000306 DASTQY010000000
VERSION     DASTQY010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478373"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1032)
                     /locus_tag="VFR82_10030"
     CDS             complement(115..1032)
                     /locus_tag="VFR82_10030"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU4501978.1"
                     /translation="MPALLLLSAYLTGDVTAQAIAESENGVRPLRVVTYNLLHDGAGS
                     GFLDGDTHLEERLEMTIRELKTLDADIIAVQEASDSRRHGNVPERLAKALGLHVVFEP
                     ATEHVFGLWPLDSIVVGMMGFKEGSAILSRFPIAASHVYDLPRCKKWLEPRIMLHAEL
                     STVWGPLQVFSTHTGRGDECQMERVGEIVRDRLGAGPSLLMGDFNTAETSQVLTMLRN
                     EAGFMDAYRLANPEAVGPTVWQRIKSPEPTVSRRVDFILLLNGRQRAASVRSSRLVLD
                     HPGHLPDGTTLWPSDHYGVLAEMDIHSVP"
     gene            1081..1665
                     /locus_tag="VFR82_10035"
     CDS             1081..1665
                     /locus_tag="VFR82_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501979.1"
                     /translation="MRIPVIVLFSACLVCGWLPLGPCMAAHADADAHGMKAGQWTGGA
                     GLGFLGNTPDGVLEFGLNGHVEYVVAERFSIGPLAQYAGAGNDFLFGLSAQAKYWWDI
                     PGSEGATKFVLQGGLGFVRAGITDTDSGTANTYGSFLIPIGIGIDHAVSPGKALTADF
                     LLNFTSLGETVRAGGREFDLHTNIMPAFYLGVRF"
     gene            complement(1697..1993)
                     /locus_tag="VFR82_10040"
     CDS             complement(1697..1993)
                     /locus_tag="VFR82_10040"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEU4501980.1"
                     /translation="MARNAFLYLLTLRLVPIFPFFVVNLLSGLTRISVGTYVAATALG
                     IIPGSLAYTFAGRQLGTINSLAELASPRLLLAFTVLGLLFLMPVVYRKFVRPQP"
     gene            complement(1994..2422)
                     /locus_tag="VFR82_10045"
     CDS             complement(1994..2422)
                     /locus_tag="VFR82_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501981.1"
                     /translation="MSRDRHVPIPESSSRPSARGKFIVAAVFLAVICAFYFFDLMAYL
                     SLDMLKANRDKFLSFTEEHYAQAVLLFILIYIAQTAFSLPGATLLTLMGGFLFGTLLA
                     ALYVNIGATTGATLAFLAARYLFRDWVEQRVGDRLAAFQA"
     gene            complement(2485..>2666)
                     /locus_tag="VFR82_10050"
     CDS             complement(2485..>2666)
                     /locus_tag="VFR82_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501982.1"
                     /translation="RQLHGTSGQLSVRKWSIMMAAHPPLSIKAPAHLNVQKSDGDIPR
                     QTGNHWRAGGLHLTR"
BASE COUNT          575 a          726 c          826 g          539 t
ORIGIN      
        1 agcaaacgac acccgtgatc agaggctgga actgtgccgt tagcggactc taggtcgatg
       61 cacctcacgc gttgggcacg gaaaggaaag ataatctctt tggcacaggt ggccctacgg
      121 aaccgagtgg atgtccatct cggcgagcac gccgtaatgg tcagacggcc agagcgtcgt
      181 cccgtccggc aggtgtcccg gatgatcgag cactagacgg ctggatcgaa ctgaggcggc
      241 ccgctgccgg ccattcaaca gcaggatgaa atccacgcgc cgcgagaccg taggttccgg
      301 tgatttaatc cgctgccaca ccgttggtcc cactgcctcg ggattggcga gacggtatgc
      361 atccatgaac cctgcctcat ttctgagcat cgtgagaacc tgggaagtct ccgcggtgtt
      421 gaagtctccc atgagcagcg agggaccggc gccaagacgg tcgcggacga tctcgccgac
      481 gcgttccatc tggcattcgt cgccgcggcc tgtgtgtgtg gaaaacactt gcagcggtcc
      541 ccacaccgtg gaaagctcag cgtggagcat gatgcggggt tccagccatt tcttgcagcg
      601 cggcaaatca tagacatgag atgccgcgat gggaaatcga cttagaatgg ctgagccctc
      661 cttgaacccc atcatgccga cgacaatgct gtcgagcggc cacaggccga agacatgttc
      721 ggtggccggt tcaaagacga cgtggaggcc gagagccttg gcgagacgct ctggaacatt
      781 gccgtgtctg cggctgtccg atgcctcttg tacggcgatg atatccgcgt ccagtgtctt
      841 caattcgcgg atggtcatct cgagtcgttc ttcgagatgg gtatcgccgt cgaggaagcc
      901 tgagccggct ccatcgtgca aaagattgta cgtcacgacg cgcaacggac gaacgccgtt
      961 ctccgattcg gcaatagcct gggctgtcac atcaccagtc agatacgcac tcaggagaag
     1021 aagcgccggc aacaaccact gcatcatacg tggtgaaaga gcatggggga ggatgacgtc
     1081 atgcgcatcc ctgtgattgt actcttttct gcctgcctgg tttgcgggtg gcttccgctt
     1141 ggcccctgca tggctgctca tgccgatgcg gacgcgcatg gaatgaaagc gggccaatgg
     1201 acggggggag cggggttggg ctttctaggc aacactcccg acggcgtctt ggaattcggg
     1261 cttaacgggc atgtcgagta tgttgttgcc gagcgattct cgatcgggcc attggcccag
     1321 tatgcgggtg ccggcaacga ctttctgttc ggtctctcag ctcaagcaaa gtactggtgg
     1381 gacattccgg gatcggaagg cgccaccaag tttgtgctcc aaggcggtct cggatttgtc
     1441 cgtgccggca tcacagacac ggacagtgga acggcgaata cctatggatc gttcttgatt
     1501 ccgatcggta ttggcatcga tcatgccgtg agcccgggaa aggcgctgac agctgatttt
     1561 cttctcaact tcacgtccct cggtgaaacc gttcgcgccg gcgggcgtga attcgatctc
     1621 cacacgaata ttatgccggc gttctatctg ggtgtccgct tctgaggcca gttcccgcgg
     1681 ggcgcggaag gttttgtcat ggttgcggac gaacgaactt tcgatagaca accggcatca
     1741 gaaagagcaa tccgagtacg gtaaatgcca ggagcagccg gggcgaggcc agctcggcga
     1801 gcgagttaat ggtaccgagc tggcgcccgg caaaggtgta agcgaggctt cccggaatga
     1861 taccaagcgc agtggctgcc acataggtgc cgacgctgat tctcgtcagc ccggagagca
     1921 gattcaccac gaaaaaggga aatatcggaa ccaagcgcag cgtgagcagg tacagaaagg
     1981 cgttccgtgc caatcacgcc tggaatgccg cgagccggtc tccaacacgt tgttcgaccc
     2041 aatcacggaa caggtagcgg gcggcgagaa aggccagcgt cgctcctgtt gtagctccga
     2101 tgttgacata cagggcggcc aacagggttc cgaagaggaa tccacccatc agtgtgagta
     2161 gggttgcgcc gggaagcgag aaggctgttt gtgcaatgta tatcagtatg aacaataaga
     2221 cggcttgcgc gtagtgctcc tcggtaaagg acaaaaactt gtcgcggttc gctttcaaca
     2281 tgtccagcga gaggtacgcc atgagatcaa aaaaatagaa cgcgcagatg actgcaagga
     2341 agacggctgc gacgatgaac ttgccacgag cggagggccg gctcgaggat tcgggtatcg
     2401 gcacgtgccg gtcacgagac attgcaaccc tcctcaattg tccgtaccgt agtttaccca
     2461 gatgggtgct tcgggcatcg tacctcaccg tgtgaggtgc agaccgccgg cccgccaatg
     2521 attgccggtt tgccgcggga tgtcgccgtc acttttctga acattgaggt gtgctggtgc
     2581 cttgatggat aggggtggat gagccgccat cataatggac cacttcctga cactcaactg
     2641 ccccgaggta ccatgaagct gtcgtc
//
LOCUS       DASTQY010000307         3357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_955127, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000307 DASTQY010000000
VERSION     DASTQY010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955127"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFR82_10055"
     CDS             <1..267
                     /locus_tag="VFR82_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEU4501983.1"
                     /translation="PYIEISNMRLGLSVKPPRFQYGVRICPEPDYWGGLSGCTYQEGV
                     SWGKFVPPEEGGRFAEVLSDATVVWPLLMAGLLERTKAKQPART"
     gene            282..908
                     /locus_tag="VFR82_10060"
     CDS             282..908
                     /locus_tag="VFR82_10060"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU4501984.1"
                     /translation="MHSALLLLVWGLLLGASGTDARPVTDDGRVQGKPDAPLTLIEYS
                     DFTCGYCLKFFQETLPRLQAAYLDTGKVKFVYRDYPRSDRGVGVEAAVAARCAGAQGR
                     YWAMHDRLFGEGRRLDSGSFKRVAKSVGLDEMQFGKCFDERRYLESIFQDRQEANRWG
                     FHGTPGFILMQTVAGPTEKVPAVAIPGAFPFEAFAEEIDRMLSKVQGS"
     gene            977..1246
                     /locus_tag="VFR82_10065"
     CDS             977..1246
                     /locus_tag="VFR82_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501985.1"
                     /translation="MSDTQSFWPVPQRDGEPPFWVCMSCLSEAFYRKVPMPDCPNCHG
                     VSTYEAFAIEAIADWGTDDLVAKAKASEQAANVEPASASSVESST"
     gene            1273..2712
                     /locus_tag="VFR82_10070"
     CDS             1273..2712
                     /locus_tag="VFR82_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU4501986.1"
                     /translation="MQGPRPFLVGGQWRQSDTRAPVYDPFTGKVLAEVSQGSEADAED
                     AIQSTAAATQPMGALPAHARFHLLQRIAGALYDRREEFARVMTGEAGKPITDARREVA
                     RAVQTFTIAAEEAKRIPGEVIPLDWTPGMDSHLGILRRVPIGPVLGITPFNFPLNLVA
                     HKVAPALAAGNPILIKPAPQTPLTALLLAEVALEAGVPQGGLNVLPCDNRVAERLVVD
                     PRFKLLSFTGSAAVGWMLKAKCGKKKVVLELGGNAGVIVEQDADLDFAAQRCASGGFG
                     YAGQTCISVQRIFVHHSVVDLFTTKLLLQVARLKGGDPSDEATVVGPLIDQHAAHRVE
                     EWIGEAVAQGARVLLGGKRMGSVVEATVLTNVTPTMKVSCQEVFGPVVTVTPYRQFDE
                     ALQAMNRSEYGLQAGVFTQDVNKVFHAFRHLEVGAVLANEIPTFRAEHMPYGGVKDSG
                     LGREGLRVSIEEMTEPRFLVLNVRPPSSA"
     gene            2851..>3357
                     /locus_tag="VFR82_10075"
     CDS             2851..>3357
                     /locus_tag="VFR82_10075"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HEU4501987.1"
                     /translation="MVPTHMFLTRGVGVHREKLASFEQALRSAGVAYCNLVSVSSILP
                     PNCKIIPRKRGEKLLNPGEITHCVMARSETNERNRLISASIGLAIPTDRRTYGYLSEH
                     HAHGETDEETGEYTEDLAAQMLATTLGVEFDPNIAWKEREQVFKMGGKIVRTLNITQS
                     AIGKPGRWT"
BASE COUNT          706 a          915 c         1025 g          711 t
ORIGIN      
        1 ccctatatcg aaatcagcaa catgcggtta gggttgagcg tcaaaccgcc gcgctttcag
       61 tacggcgtgc gtatctgtcc tgagccggac tactggggag gattgagcgg ctgcacatac
      121 caggaaggag tgtcttgggg aaagttcgtg ccgcccgaag aaggcgggcg ttttgctgaa
      181 gtactgagcg atgccacggt cgtctggccg ttgctgatgg ccggcttgct cgagcggacc
      241 aaggcaaaac aaccggctcg gacatgaagg ggacgactgt tgtgcactcg gccttgctgt
      301 tgctagtctg gggactactg ctgggagcat ccggcacaga cgcccgaccg gtgaccgatg
      361 acggacgtgt acaaggaaaa cctgatgcgc cgctgacgtt gatcgaatat tctgacttta
      421 cctgcggcta ttgcttgaag ttttttcagg aaaccttgcc gcggttgcag gcggcttatc
      481 ttgatacggg gaaagtaaaa ttcgtttacc gcgactatcc tcgatccgat cgcggtgtcg
      541 gcgtcgaggc ggctgtcgca gcccgttgcg ccggagcgca ggggcgctat tgggccatgc
      601 atgatcggtt gttcggcgaa gggagacggt tggattccgg gtcctttaag cgggtagcca
      661 agtctgtggg attggacgag atgcagtttg gaaagtgttt cgatgagcgg cggtacctgg
      721 aatcaatttt tcaagaccgg caggaggcga atcgttgggg atttcacggt acaccgggat
      781 ttattttgat gcagaccgtc gctggcccga cagaaaaggt tcctgccgtc gcaataccgg
      841 gggcgtttcc gtttgaggcc tttgctgaag agatcgatcg tatgctgtcc aaggtacagg
      901 ggtcgtagtc gtcaccgcgc agccgagatc gaacgagaat tacccatcac gttctccata
      961 cacgaaggat taccgtatgt ctgatacaca gtctttctgg cctgtccctc agcgcgatgg
     1021 tgaacctccc ttctgggtgt gcatgtcgtg tctttcagaa gcgttctatc gcaaagtccc
     1081 gatgccggat tgccctaatt gtcacggggt gtccacctac gaagcatttg cgatcgaagc
     1141 catcgccgat tgggggaccg atgacttggt cgccaaagcc aaggcttctg aacaagcggc
     1201 gaatgtcgaa cctgcgtccg catcctcagt ggagtcttcg acgtaagccg atcgcccaag
     1261 cggagtgtct ttttgcaagg accacgtcca ttccttgtcg gcggccaatg gcgccaaagt
     1321 gacacacgcg ctccggtgta cgatcccttc accgggaaag tcctggctga ggtttcgcaa
     1381 ggcagcgagg ccgatgccga ggatgccatc caatcaacgg ccgctgcgac tcaaccgatg
     1441 ggcgcgctgc ctgcgcatgc ccgatttcac cttttgcaac gcattgccgg agcgctctac
     1501 gatcgacggg aagaatttgc cagagtcatg actggcgaag cgggaaagcc cattacggat
     1561 gcccgtcggg aagtcgcgcg tgccgttcaa accttcacca tcgcagcgga agaggccaag
     1621 cgaatacctg gcgaggtgat cccgctcgac tggacgcccg gcatggattc tcatctgggc
     1681 attcttcgtc gggttccgat cgggccggtg ctcggaatca cgcccttcaa ctttccgtta
     1741 aatcttgtgg cccataaggt cgcgcctgct ttggctgccg gcaatccgat attgatcaag
     1801 ccggcgccac aaactccttt gactgccctg ctgttggctg aggtggcact tgaggctgga
     1861 gttccgcaag gcggtctcaa cgtgttgccc tgtgacaacc gcgtcgctga gcgtctggtc
     1921 gtggatcctc gcttcaagct cttaagtttc accggcagtg cggcggtcgg atggatgttg
     1981 aaggccaagt gcggcaaaaa aaaagtcgtc ttggaactgg gaggcaatgc cggcgtcatc
     2041 gtggaacagg atgccgacct ggattttgcg gctcaacgct gtgcgagcgg aggattcggc
     2101 tatgcggggc aaacctgcat ttccgtccag cgcatttttg tgcaccactc ggtggttgat
     2161 ctcttcacga cgaagctgtt gctccaggtg gcccgtttga aggggggcga tcccagcgac
     2221 gaggcgacgg tcgttggccc gttgatcgat caacacgcgg cgcatcgcgt cgaagaatgg
     2281 atcggagaag cagtcgccca gggtgcacgg gtgctcttag gcggcaaacg catgggttcc
     2341 gtcgtggaag ccaccgtgct gaccaacgtc acgccgacaa tgaaggtctc ttgtcaggaa
     2401 gtgttcggtc cagtggtcac cgtgacgccc tatcggcagt ttgacgaagc tcttcaggcc
     2461 atgaaccgtt ccgagtatgg gctgcaggcc ggcgtcttca cgcaagacgt caacaaggtc
     2521 tttcatgcct tccgccattt ggaagtcggg gccgtgcttg ccaacgagat tccgacgttc
     2581 cgggccgaac atatgcctta cgggggcgtg aaggactccg gcctaggccg ggaggggttg
     2641 cgcgtatcga ttgaagagat gaccgaaccc cgattcctcg tcttgaacgt caggccgccg
     2701 tcgagtgcat agcgaaaaaa gcagtcagga tattgcgatc cgcggacatt cttgatacaa
     2761 cagcgaccct gtgcctagaa aactcgcgcc agccttgacg tttgtggccg actgcaatgc
     2821 cgaccggacg aagcaaggga gaaggagacg atggtaccta cgcacatgtt tctgacaaga
     2881 ggagtcggcg tccaccgaga aaagctggcg tccttcgagc aagcacttcg cagcgccggc
     2941 gtcgcctact gcaacctcgt cagcgtctcg tcgattctcc cgcccaattg caagattatc
     3001 ccccggaagc gcggcgaaaa actcctgaat cctggcgaaa tcacgcactg cgtcatggcg
     3061 cgttcagaga ccaacgagcg taaccgtctc atttccgcgt cgatcggatt agcgattccg
     3121 acggaccgcc gcacatacgg ctatttgtcc gagcatcacg ctcacggaga aaccgacgaa
     3181 gaaacggggg aatatacaga agacctcgct gcgcagatgc tggccacgac gttgggggtg
     3241 gaattcgatc ccaacatcgc ttggaaagaa cgagaacaag tgtttaagat gggtggaaag
     3301 atcgtgcgga ctctgaacat tacgcagtcg gccattggaa aacccggccg atggaca
//
LOCUS       DASTQY010000308         2304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_937085, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000308 DASTQY010000000
VERSION     DASTQY010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937085"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFR82_10080"
     CDS             <1..199
                     /locus_tag="VFR82_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501988.1"
                     /translation="TKLGLNAQLGLRYYLTRHVTMFAEGKYNHVRFNFPETPPGSSFN
                     LFGFNATYNMVHVAFGVSYHF"
     gene            262..612
                     /locus_tag="VFR82_10085"
     CDS             262..612
                     /locus_tag="VFR82_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501989.1"
                     /translation="MKVCPSCRREWPEIYRYCTHCGSQMDGTPRPIARASIVPEGLNL
                     HILYGMVAILVLALLFPPWETPPAQPPQFLGLHFILAPPTPDAVVSRLLITIELVTIA
                     IAGLYGAFLFRSGR"
     gene            complement(642..1442)
                     /locus_tag="VFR82_10090"
     CDS             complement(642..1442)
                     /locus_tag="VFR82_10090"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEU4501990.1"
                     /translation="MSRLLVCPPDYFCIDYEINPWMRRSNVVAPEQAIRQWHRLMEVL
                     EGDVGAVVERMKPVPGLPDLVFTANAGVVVGRKAVPSRFRYPERQREEAHYEGWFRDQ
                     GYEIITLDPDMFFEGAGDLLGFHDGWFGGYRQRSDIRAFPVLSELFGKEILPLELIDG
                     RFYHLDTCFCPLSGGDLLYYPAAFDRYAQAAIADRIPTASRLPVPEQEALQFACNAVC
                     VGKHVVLPDGCPETMTWLECRGYLPHPVPLDEFMKSGGSAKCLTLALD"
     gene            complement(1439..>2304)
                     /locus_tag="VFR82_10095"
     CDS             complement(1439..>2304)
                     /locus_tag="VFR82_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEU4501991.1"
                     /translation="VSKDRTKAFAVPMAGVKVGDEVVTGRTGIRVVPLQRPQERDVFS
                     FMESQVSAERPHGHIIADIAKRMEVLRDGHRGGKSGCKVLVAGGPAIIHAGGRDALTW
                     LIDQGFVHVLFCGNALPAHDMEADLYGTSLGYGLSAGRVVPHGHEHHLRTINRIRGIG
                     SIAKAVETGVIKQGIMASCVRQRVQTVMAGTIRDDGPLPDVITDSVAAQAAMRAAIPG
                     VGLALLVASTLHAVATGNLLPATIPTVCVDVNPAVPTKLADRGSFQAVGLVMDAASFL
                     RDVARELGWKG"
BASE COUNT          480 a          729 c          633 g          462 t
ORIGIN      
        1 cacgaaactt ggactcaacg cgcagctcgg actccgttat tacctcacca gacatgtcac
       61 gatgttcgcc gaaggaaaat acaaccacgt gcggttcaat ttcccggaaa ctccgccagg
      121 cagcagtttc aatctgttcg gcttcaacgc cacatacaac atggttcacg tcgccttcgg
      181 cgtcagctat catttttgat cgaagaccct ggttgtacag ttggaaaaga gacgggttcc
      241 gtgtagattg cacggatccc aatgaaagtc tgcccgtcat gccggcgtga gtggcctgag
      301 atctatcgct actgcaccca ttgcggcagc cagatggatg ggacccctcg accgatcgct
      361 cgcgcatcca tcgttccgga aggcctcaat cttcacatct tgtatggcat ggtggctatt
      421 ctagtcttgg ctctcctttt cccgccgtgg gagacacctc cggcacagcc tccccaattc
      481 ttggggctgc atttcatcct ggcgccgccg acgcctgatg ccgtcgtcag ccggctcctg
      541 atcacgatcg agctggtgac gattgccatc gcgggcctct atggagcgtt tctgtttcgc
      601 agcgggaggt aagaggatgc gtgtcctggt tcggttgacg ctcagtccag agctaatgta
      661 aggcacttcg cggaccctcc ggatttcata aattcatcca aaggaaccgg gtgtggcagg
      721 tacccgcggc actcgagcca ggtcatcgtc tctggacaac cgtccggcag caccacgtgc
      781 ttccccacgc aaacggcatt gcaggcaaat tggagcgctt cctgctcagg aacaggaagc
      841 ctcgacgccg tcgggatacg atcagcaatt gctgcctgcg catagcggtc aaacgccgcc
      901 ggataataga ggagatctcc cccgctcaaa ggacaaaagc aggtgtcgag atggtagaac
      961 cggccgtcaa tcagttccag agggaggatc tctttaccga acaattcgct gagaacaggg
     1021 aatgcgcgga tgtccgaacg ctgcctgtat ccgccgaacc agccatcgtg gaaccccagc
     1081 aggtcgccgg ccccttcgaa aaacatatcc gggtccagcg tgatgatttc atacccctgg
     1141 tcacggaacc agccttcgta atgcgcctct tcccgttgcc gctccgggta gcgaaaacgg
     1201 ctgggaacgg ccttgcggcc gaccacgacg cccgcattgg cggtaaagac cagatctgga
     1261 agcccgggaa ccggcttcat ccgttccacc accgcgccaa cgtccccctc gaggacctcc
     1321 atcagccggt gccactgtcg aatggcttgt tcaggagcga cgacatttga acgtcgcatc
     1381 cacggattga tctcatagtc aatgcagaaa tagtccggcg gacagacgag taaccggctc
     1441 atcctttcca ccctagttct ctcgccacat cgcgcagaaa tgaggcagca tccatcacaa
     1501 gtcctaccgc ttggaaactg ccgcgatcgg ccagcttcgt cggcactgcc ggattcacgt
     1561 cgacgcacac cgtcgggatg gtcgccggta ggagattgcc ggtggcgacc gcgtgaagcg
     1621 tagaggcgac cagcaaggcg aggcccacgc cggggatcgc tgcacgcatc gcagcctgcg
     1681 ccgcaacgga atcggtgatg acgtccggaa gcggcccatc gtctcgaatg gtaccggcca
     1741 ttaccgtctg cactcgctgt ctgacgcagg aggccatgat gccttgttta atcacgccgg
     1801 tctcgacggc tttcgcgatg ctgccgatgc cgcgaatgcg gttaatggtg cgcaaatggt
     1861 gctcatgtcc gtgcggcacg accctgccgg ccgaaagtcc ataccccaat gaggtgccat
     1921 acaaatcggc ctccatgtca tgtgccggaa gcgcattccc gcagaacagg acatggacaa
     1981 atccttggtc gatgagccag gtcagcgcat cgcgccctcc ggcatggatg atcgcaggcc
     2041 ctccggcgac caacaccttg cacccggatt ttccgccccg gtgaccgtca cgcagcacct
     2101 ccatccgttt cgctatgtcg gcgataatat ggccgtgggg ccgctcggcg gatacctgcg
     2161 actccatgaa actgaatacg tcacgctctt gaggccgttg caatgggacc acgcggattc
     2221 ccgttcggcc agtcaccact tcgtcaccga ctttgacgcc ggccatcggt acggcgaaag
     2281 ctttggtcct gtccttactc acca
//
LOCUS       DASTQY010000309         6625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_743212, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000309 DASTQY010000000
VERSION     DASTQY010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743212"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /locus_tag="VFR82_10100"
     CDS             <1..1639
                     /locus_tag="VFR82_10100"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU4501992.1"
                     /translation="SLRVFLIHVRDPQFYALPAKTRAKNGNIRVMGFPPIGIMSLSAV
                     VKAAGHECVMFDQANPDTPNDVIVEAIKQQKPALVGLSFLSTTSYPYAKMLARQIRAA
                     DATVKLAFGGVFASLNAGLVKLQCPEVDFVCRGDGEQLLLDLLAQMDAPETVGGLTWM
                     KDGQVMTNPGRPMERHLDQWPFPDRESLPLDFVESMPLDVPAVLSMERFTTMQTSRGC
                     PWPCVFCDIPIFNEGKWRARSAAHVVAELKYLEAHGYGAVYFVDDHFLLQPKRIEAIC
                     TGIQQEHLRIQWGIEGRVDSVAQHLFPAMAQAHCRTIMFGIESGSQRILDRLKKEQTL
                     DEVTQAVTNAKKAGIEIVHGFFTVGNPDETVEDMKATFEFAARLPLDTFGFNRLCVYR
                     GTPLWQEYVKRGLVQDATDWYKYFKCSEIDPTCLPGEVINRVRQAGLKRLFLYKFVHH
                     PRQTYRLLRRFLRFMPVKDVLYLLFKPFLGQKTGATKAEVLSRAVEHAEMKDAAAQLT
                     HLSDERLLDVLETARQERVRLQRDSEQRREIPMVPAR"
     gene            1898..2293
                     /locus_tag="VFR82_10105"
     CDS             1898..2293
                     /locus_tag="VFR82_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4501993.1"
                     /translation="MTKAPRIRVLLVDDHSLFRQTLRSLLAPYPNLELVGEASDGHEA
                     VESVARLQPSVVVMDIHLQRMMDGIAATHLITSQNPGVAVVGLSCDTRQYVVSAMQQA
                     GASEVLAKEQGADDVYGAIQRASASMPDE"
     gene            complement(2384..3052)
                     /locus_tag="VFR82_10110"
     CDS             complement(2384..3052)
                     /locus_tag="VFR82_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501994.1"
                     /translation="MQTLDLHRPHMPDLQFVLLVTALCTSRLPALNVPDGLRTTIFDR
                     CWMLIHDCPPPRKPEERVLDLRPWSEMTVEAMVETIRVTLIEAGIETLTWDHPSSEPT
                     RTSPPEAQMLIDRLERLYPHPSEGKDAPRGETLETTGVHPDPVENEVGQLIGEMATLM
                     AALAPAMKRRAADLAAEGKADEVVDKLLKGAEVMHGSGHMYLTWARHYASLSENSGEE
                     AQET"
     gene            complement(3072..3308)
                     /locus_tag="VFR82_10115"
     CDS             complement(3072..3308)
                     /locus_tag="VFR82_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501995.1"
                     /translation="MANHTEALTPVNSQYTLGYGMRLDKGTAPELAHPNVSVTLPDGS
                     EGHMTLHVINGTPDEIKARLLESVDAFFDIHAEL"
     gene            complement(3457..3612)
                     /locus_tag="VFR82_10120"
     CDS             complement(3457..3612)
                     /locus_tag="VFR82_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501996.1"
                     /translation="MSLDNVLFLPRLDAFMPKDHRDVVEHGPLGKLGRYGSQEFNQKP
                     AAAIGTA"
     gene            complement(3657..4559)
                     /locus_tag="VFR82_10125"
     CDS             complement(3657..4559)
                     /locus_tag="VFR82_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU4501997.1"
                     /translation="MSKERITISPDLYDIMPLDYQDLVNHATYGKEDRGWKDIGSSKE
                     LIEQHSLCAGCPESMAFRYVLAALPNPEDTVMVGSTGCTSLVFPHVAIHNIHSLFGNQ
                     NAVASGLKRALTVRFPNRIKDVVVLAGDGATVDIGLDMTLQAWFRQEKFTTICFDNEL
                     YANTGGQESGLMQKGFVAKMAPVGKLFDKVRLPEIARESGCHYIVNCTVSKPSVVEKV
                     IRNAVLIAREIGPTYVQLYTPCILEIGKDSMEGLQEMRDSEKPNERFAFKEYVSDPAK
                     ALLADLAAKAKERKAAAKAVLQEA"
     gene            5273..5440
                     /locus_tag="VFR82_10130"
     CDS             5273..5440
                     /locus_tag="VFR82_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4501998.1"
                     /translation="MLYYALVFLTVGLITGALTITGMSSLPVEIPLVLSLTGIVLVAT
                     YMFIKRTARIA"
     gene            5516..5755
                     /locus_tag="VFR82_10135"
     CDS             5516..5755
                     /locus_tag="VFR82_10135"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEU4501999.1"
                     /translation="MVKNKVSNPTTPGVSAGTSEGSQEDEQSDPPFQISRAGWEYERI
                     ARRAYELYEQRGHQEGRDVEDWMKAEQQLAREAGK"
     gene            5811..6293
                     /locus_tag="VFR82_10140"
     CDS             5811..6293
                     /locus_tag="VFR82_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU4502000.1"
                     /translation="MNTLLRWDPTSRTRWNPFKHRDQDELESRLATLLAGREATANGG
                     NEALTVTQWSPLVDITEDDKEYLIKAELPDMKKEDVRLTVEDGVLSIAGDRKFEKEEK
                     GRKYHRIERAYGSFVRSFSLSEDADGRNVTANFKDGVLQVHLPKSVKAKQKAVEIKVG
                     "
BASE COUNT         1381 a         1880 c         1945 g         1419 t
ORIGIN      
        1 ttctttgcga gtatttctga tccacgttcg cgacccgcag ttttacgcgc ttcctgcgaa
       61 gacccgcgcg aaaaacggca acatccgcgt gatgggcttt ccgcccatcg ggatcatgtc
      121 gttgtccgcc gtggtgaaag cggcgggaca cgaatgtgtg atgttcgatc aggcgaaccc
      181 ggacacaccc aacgacgtca tcgtcgaggc catcaaacag cagaaaccgg cgctcgtcgg
      241 cctcagcttc ctgagtacga ccagctatcc gtatgccaag atgctcgccc ggcagatccg
      301 cgccgccgat gccacggtca agctcgcgtt cggcggagtg ttcgccagtc tgaatgccgg
      361 cctcgtcaaa ctccaatgcc cggaagtcga tttcgtctgt cgcggggacg gcgaacaact
      421 gctgctcgat ctgctcgccc agatggatgc ccccgagacg gtcggcggcc tgacgtggat
      481 gaaggacggc caggtgatga ccaatcccgg ccgtcccatg gaacggcacc tcgatcagtg
      541 gccctttccg gatcgcgaaa gcctgccgct ggatttcgtc gagtcgatgc cgctggatgt
      601 cccggcggtg ctctcgatgg agcggtttac gaccatgcag acctcccgcg gctgcccctg
      661 gccgtgcgtg ttttgcgaca ttccgatttt taatgaaggc aaatggcggg cccgcagtgc
      721 cgcgcatgtg gtcgctgagc tcaagtatct cgaagcgcac ggctatggcg ccgtctattt
      781 tgtcgacgac cactttctcc tccagccgaa acgcattgaa gcgatctgca cggggatcca
      841 gcaggaacac ttgaggattc aatggggcat cgaaggccgg gtcgactccg tcgcgcagca
      901 tctcttcccg gcgatggccc aggcccattg ccggaccatc atgttcggga tcgagagcgg
      961 cagccagcgg attttggatc ggctcaaaaa ggaacaaacc ctggacgaag tgacccaggc
     1021 ggtgaccaat gccaaaaaag ccggcatcga aatcgtccac gggtttttta ccgtggggaa
     1081 ccccgatgaa accgtagagg acatgaaggc gacgttcgag tttgccgccc gcttgccgct
     1141 ggatacgttc ggctttaacc ggctgtgtgt gtaccgcggc accccgctct ggcaagagta
     1201 tgtgaaacgc gggctcgtcc aggatgcgac ggattggtac aagtacttca agtgctcgga
     1261 aatcgacccc acctgcctgc cgggggaagt gatcaatcgc gtccggcagg cagggttaaa
     1321 acggctgttt ctctataaat tcgtgcacca cccccggcag acctaccgcc tcctgcgccg
     1381 gtttctgcgg ttcatgcccg tcaaggacgt cctgtatctc ctgtttaagc cgttcctcgg
     1441 ccaaaagaca ggcgccacca aggcggaagt gctatcgcgg gcggtcgagc acgccgaaat
     1501 gaaagacgcg gcagcccagt tgacgcacct gagcgacgaa cggctgctgg atgtgctgga
     1561 aacggctcgg caggaacgcg tccgccttca acgggactcc gagcagcgca gagaaattcc
     1621 catggttccc gcccggtgat caccattcat gacgaaaatg tcgatccttt tcggccagag
     1681 tgtgcgtgat tccaatcgct tcccgaagga agccgccccg ggctgacgtc ccactcaccc
     1741 gagaaacact gaccatcatc tccaataagg gtggtgcatg ttgatttagg ttctttgcgt
     1801 actcactctg cataggcgaa cctcgccaag cagctgggcg acgatgaagc aattttgcaa
     1861 actcacggac ctgactctgt tttttcgagg gaacactatg accaaagcac cacgcattcg
     1921 agtactactt gtcgatgatc actcgctgtt tcgtcagacc ctgcgcagtc tattagctcc
     1981 gtaccccaac ctcgagcttg tcggcgaagc gagtgatggt cacgaagccg tcgagagtgt
     2041 ggcgcgacta caaccatctg ttgtcgtgat ggatatccat ctgcagcgga tgatggacgg
     2101 aatcgcggcg actcatctga tcaccagtca gaatccaggg gtggctgttg ttggcttgtc
     2161 ctgtgatacc agacagtatg tcgtctcggc gatgcagcaa gcaggggctt ccgaagtgct
     2221 cgcgaaagag cagggcgctg atgacgtgta tggtgcgatt cagagggcgt cagcgtcaat
     2281 gccggatgag taacttagtg tgctgttgtc cctccagagc tattacggga ctcgaaaaca
     2341 cgacaaactg aagtccgctg tgatcaatag cagtcgttca tgctcaggtt tcttgggcct
     2401 cctcgcccga gttctcactg agggaggcat agtggcgtgc ccacgtcaga tacatgtgtc
     2461 cgctgccgtg cattacctca gcgcccttca gcaacttgtc gaccacctcg tcagcttttc
     2521 cttccgccgc gaggtctgcg gcgcggcgtt tcattgcggg ggctaatgcg gccatcaatg
     2581 tggccatctc cccgatgagt tgccccacct cattttccac gggatccggg tggactcctg
     2641 ttgtctccaa cgtctctccc cttggtgcgt cttttccttc ggaggggtgc ggatacaggc
     2701 gctcgaggcg atcgatgagc atctgcgcct ccggcgggct ggtgcgtgtc ggttcgctgg
     2761 acgggtgatc ccaggtcagc gtttcgatgc ctgcttcgat gagtgtcacg cggatggtct
     2821 ccaccatcgc ctccacggtc atctccgacc acggccgcaa gtctaacacc cgctcctcgg
     2881 gcttgcgcgg cggcgggcag tcatggatga gcatccagca gcggtcgaaa atcgttgtgc
     2941 gaagcccgtc cgggacgttg agcgcgggca ggcgagaggt gcagagtgcc gtcacaagca
     3001 ggacgaactg aagatcaggc atgtggggac gatggagatc tagcgtctgc atggggtgtc
     3061 ccgtcttctt ctcagagctc ggcgtggatg tcgaagaacg cgtcaacgct ttcgagcaat
     3121 cgcgctttga tttcgtccgg cgtgccgtta atgacgtgta aggtcatgtg tccttccgat
     3181 ccgtccggta gtgtcacgga cacattcgga tgcgccagct ccggagccgt acccttgtcc
     3241 agccgcatgc catagcccag cgtgtattgc gagttgacgg gcgtgagagc ttctgtgtga
     3301 ttagccatgg atgctccttc tgccctatgg cagtgtgttg agacgggtga caacgccgtt
     3361 cattccattt cgtgtttgcg ctgtaggtca taccgaccct cctccaatct aacccgtgaa
     3421 agtgagaaag agggctgcag gggagccttc tcagactcat gccgttccaa tcgccgccgc
     3481 cggcttctga ttaaactcct gggatccata ccgccccagc ttgcccaagg gcccgtgctc
     3541 cacgacgtcg cggtggtcct tgggcatgaa cgcgtcgagt ctcggtaaaa acagcacatt
     3601 gtcgagactc atccgatacc tcgctctctc gctgagccgc aatcgcctcg gtggatttac
     3661 gcctcttgca acaccgcctt cgcggccgcc tttctctcct tcgccttggc ggcgagatcc
     3721 gccagcagcg ccttcgcggg atcgctgacg tactccttaa acgcaaaccg ctcgttaggc
     3781 ttttctgaat cacgcatttc ctgcaagcct tccatgctgt ccttgccgat ttcgaggatg
     3841 cacggggtat agagctggac gtaggtcggg ccgatttccc gggcaatcaa gaccgcattg
     3901 cggatgactt tctcgacgac cgagggcttg ctcaccgtgc aattcacgat gtagtgacac
     3961 ccggattccc gcgcgatctc ggggaggcgg actttatcga agagttttcc gacgggggcc
     4021 attttggcca cgaagccctt ctgcatcagg ccgctttcct gcccaccggt attggcgtac
     4081 aactcgttgt cgaagcaaat ggtcgtaaac ttttcctggc ggaaccaggc ttggagcgtc
     4141 atgtccaggc cgatgtccac cgtcgcgccg tctcctgcca gcacgaccac atccttgatg
     4201 cgattcggaa accggaccgt caaggcccgc ttcaggccgg aagccaccgc gttctgattg
     4261 ccgaacaatg aatggatgtt gtggatggcg acgtgcggaa agaccaggct ggtgcaaccc
     4321 gtcgagccga ccatgacggt atcttccggg ttgggaagtg cggccagcac ataacgaaac
     4381 gccatggact cgggacatcc ggcacagagt gagtgctgtt caatgagttc cttcgagctg
     4441 ccgatgtctt tccatcctcg gtcttccttg ccataggtgg catggttgac gaggtcctga
     4501 taatccaatg gcatgatgtc gtaaagatcc ggtgatatgg taatgcgttc cttgctcatg
     4561 gtgtctcctt gtagaagtcc gattgcgccg tcgctcggac aagtgtacga atgggctcta
     4621 ccgcgaagtg atgtatgccg cagccaccag cgctcctgac atgcatacca agaaaagtgc
     4681 gagcaagagt tccggtgcca tgtgacgaat aaagtccatc tgcgacaatc ctcgatcgac
     4741 ttgcgtgccg aggcagtgtc ctcgccagag gaatggcatt cgcctccggt catgcgaaag
     4801 cacggcacgc ggtgtaaaga tattcggcgt agaagcggcg gagaccagga aatcaatcgc
     4861 gcaggaggag gggggacgcg cgagggatag gacagtcggc gtggctcaat acgtcatacc
     4921 ttcacgctaa gcgctataga cacaggtgtc aagcggtata aagggtagct gcgttattag
     4981 ttcgtcattg tgatcgacta atccgactct ctcatattat agcgcttgac tccctgaccc
     5041 attgcgccca gggttgttgg tgaggtgtaa cactccgttg actgtcctat cctcccgctt
     5101 cctactcctt cccagcgaga ccgattcccc agtctccgcc gttttgatac accgggtgcc
     5161 ctgactcatt tgcctgtccg gtttcctgat ccgctgatgg aggcggggaa ggctccggcc
     5221 actgcgtcag aggcggtcta ttcacactcg cttccataga gaggagtgaa ccatgctgta
     5281 ctatgcacta gtctttctga cggtcggtct gataaccggc gccctgacta tcaccggaat
     5341 gtcttcgtta ccggtcgaaa ttccgttggt gttgtcgttg accgggatcg tgttggttgc
     5401 gacatatatg ttcatcaaac gcaccgctcg gattgcgtga cgaggctgcg gaagaggatg
     5461 aagctgatcg actcaatgcc accacgaacc atacgggagc gtgaggaggt gcacgatggt
     5521 caaaaacaag gtttccaacc caacaacgcc gggtgtatcg gccggcacat ccgaaggcag
     5581 tcaggaggat gaacagtccg atccgccctt tcagatatcg agggccggat gggaatacga
     5641 gcgcattgcc cggcgggcct acgagctgta cgaacaacga gggcaccagg aaggccggga
     5701 cgtggaggac tggatgaaag cagagcagca actggcccgc gaagcgggga agtaatcaca
     5761 tgaagcggtg atcgaatggg aactccattc gacattaaag gagacgagcc atgaacacac
     5821 tattacggtg ggaccccacc agcagaactc gatggaaccc gttcaaacat cgcgatcaag
     5881 atgaattgga aagccggttg gcgacgctgc ttgcgggccg ggaagcgacg gcgaacggag
     5941 ggaatgaggc cctcacggtc acccagtggt cgccgctggt ggacatcacc gaggatgaca
     6001 aggaatacct catcaaagcc gagttgccgg acatgaagaa agaggacgta cggctcacgg
     6061 tggaagacgg cgtactgtca attgccggcg atcggaaatt cgagaaggaa gaaaagggaa
     6121 gaaaatatca ccgcatcgaa cgcgcctacg ggagcttcgt tcgaagcttc tccctttcgg
     6181 aggatgcgga cgggcgcaat gtgaccgcca atttcaagga cggcgtgctc caggtgcatc
     6241 ttcccaaatc cgtcaaggct aaacagaaag ctgtggagat caaggttggc tagtggtgct
     6301 gattcgtggg cgtcggtgaa ggcttcgtac gcgcgtgcga cgatggagtc ttgctgacgc
     6361 tccacgcaga tcctgtccaa agggattgaa gagcacgctc atcctcggag gccgaccggg
     6421 gctggatgag cggcgtccac gggtgataag gaccatcatc tcccttcttg ccaaaaagga
     6481 atgtacacca tgactatcgt gagtgaaaat tgggctcttg cggcaacact ctttgccctt
     6541 atgatgggca tcctcggagc ctatttcgtg ctgctttggt atgtgttggg tagtcaataa
     6601 ggacgtagac tattcatcgt tgaat
//
LOCUS       DASTQY010000310        23811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1273433, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000310 DASTQY010000000
VERSION     DASTQY010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 23811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23811
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273433"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VFR82_10145"
     CDS             <1..788
                     /locus_tag="VFR82_10145"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU4502001.1"
                     /translation="YPQPCRRPTDGRYGENPNRMQHYYQYQVVLKPSPDNIQELYLDS
                     LARLGIDPKKHDIRFVQDDWESPTLGAWGLGWEVRLDGMEITQFTYFQEIGGIELSPI
                     TGEITYGTERIAMYLQQVNNVFDLTWTDGMSYGDIHHETEVQFSRYNFEQGDVAMLMN
                     AFQSYEAECKRLLSQSDQRLTLPAYDYCIKSSHAFNLLDARGAISVAERTGYIARVRA
                     LARQCAERYIEERASMGHPLIKHHQAARAGRRPTAGVGRSTRR"
     gene            785..2959
                     /gene="glyS"
                     /locus_tag="VFR82_10150"
     CDS             785..2959
                     /gene="glyS"
                     /locus_tag="VFR82_10150"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU4502002.1"
                     /translation="MTRRKAPTGRLQVSSASGAELLFEIGVEELPYQVIAPAMSALKE
                     VAGRLLEEARLTIESVNVYGTPRRLTLVMSGLALRQTSAKKEVMGPSKVVGFDSQGQP
                     TKAAIGFAAGQGLSVERLEVRRTAKGDYLFAVKEEAGRPTQAVLLELLPQLMGKLSFP
                     KAMKWNETATRFARPVRWLIALFNGALLPIEAAGIRAGDRTYGHRVMGQRKPLVVRNY
                     ESYRSGLERAGVLVDPVTRRQVIEQQLHQICSRAAVELNADESLMDQAVFTTEWPCAL
                     LGSFKPEYLSLPEEILMTSMKEHQGFFSVRDKTSGKLAPHFLAVANNRPKDMSLIRAG
                     NERVLAARLADAKFFFDEDRRTRLEDRVKKLSGVTFHQKLGTMGQKAERLVQLVALIA
                     SGIHRDSESQDFVNACRRAAHLSKADLLTGIVGEFPELQGIMGGEYAKHDGEPEAVSQ
                     AIREQYLPKALEGELPRSLPGLVLSLADRLDTTAAFFHVGMVPTGSEDPFALRRHATA
                     IVRIVLEKELRLDLGKYIDYARTLLVDSGIKGAPDSEQHGRRRVTDFVFERVRHYGRT
                     VHAFRDDVMDAVLRQVSAGPIDLVDLLLKLKAVQGVTAKADFDPLIVGFKRASRIIEK
                     EQWDRKPVDPGMFQEAGEHELHRQIRACQHDFQQHMAEGCYDRALDALVRLKPAIDAF
                     FEGVMVNVEDAALRSNRLSLLKDVSDCFTSFADFSRIVVQGG"
     gene            3077..3574
                     /locus_tag="VFR82_10155"
     CDS             3077..3574
                     /locus_tag="VFR82_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502003.1"
                     /translation="MIASSEYDLEEKRAAKIRRFASVVTVGLIVFCNVLLLFGVWVSG
                     VNLDELVKTPDLFDAKEDVCLRLSWERVSGAADPVRLCSEWIKLADPSGKPHQLAKDI
                     AVRRGPDGQYYVDRGVRPDFRLLGLALFVGVVLLFGMWARRYLVSRYRLQIGPADSRS
                     SSGLH"
     gene            3598..6375
                     /gene="ppdK"
                     /locus_tag="VFR82_10160"
     CDS             3598..6375
                     /gene="ppdK"
                     /locus_tag="VFR82_10160"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEU4502004.1"
                     /translation="MAKKYVYYFGDGKAEGTSNMKELLGGKGAGLAEMTNLGISVPPG
                     FTISTEACVEYYKLGKKYPPGMWEASLQALKRVERSMGMGFGDPERPLLVSVRSGARA
                     SMPGMMDTVLNVGLTTKTVEGLAAKTRNDRFAQDSYRRFVSMFGSIVMGVHREHFEAI
                     LKHKKAEVGVTHETHLDARHLRELVASFKALVKEETKKEFPDEPLDQLRMAISAVFSS
                     WFGARAITYRRLYGIPDSWGTAVNVVAMVFGNMGETSGTGVAFTRSPSSGDKAFFGEC
                     LMNAQGEDVVAGIRTPLPVNALAKNVPEAYRELEQTYKKLEKHYRDMLDLEFTIQEGK
                     LYMLQTRVGKRTGIAAVKIAVDMVKEGAISREEAVLRVGPDQLAQYMYPIFDMKEESK
                     ANPLGKGLPAGPGAAAGKIALTPDRAVDMKAAGSRVVLVRQETSPDDIHGMNAAGGFL
                     TARGGMTSHAAVVARQMGKVCVAGCDSVEVLDSQTVKIGAKTFREGDYLSINGSTGNV
                     YEGDIPVVDSEIIQVIQGKLDPKKSEKYQRFSTILSWADSLRRLRVRANADVPEQAKI
                     ARGFGAEGIGLCRTEHMFFAEDRIPLMQKMILARTTEEREKYLDQLRPLQKQDFIGLY
                     REMEGFPVTIRLLDPPLHEFLPKREDLMVEIAQLELTGKDAAKLEENRRLLARVEELH
                     EFNPMLGLRGCRLGITMPEITRMQARAVIEAACELAKEGKKIVPEIMIPLVGMVSEMK
                     SQKDLVREVAQETMKRYNVKLSYLVGTMIELPRAAVTADRIAEEAEFFSFGTNDLTQT
                     TFGFSRDDAAKFIDHYKTANLMESDPFAVLDREGVGCLMSQAIAGGRKTRTGIKLGIC
                     GEHGGDPSSVEFCHQLGLDYVSCSPYRVAIARLAAAHAALAEADARKAAKATLRKTTR
                     RKQSTARRKQR"
     gene            6497..7618
                     /gene="ribD"
                     /locus_tag="VFR82_10165"
     CDS             6497..7618
                     /gene="ribD"
                     /locus_tag="VFR82_10165"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU4502005.1"
                     /translation="MRAGHAQDLHYMKFALALAANGRGTASPNPMVGAVVVSHGRIVG
                     KGYHLRPGLPHAEILALKAAGPQARGGTLFVTLEPCCHLNKRTPPCVPAVIWAGVTRV
                     VIAQRDPNPLVNGKALKALRRAGVSVTVGVGKSEAIELNRAYNHYMRTRRPYVLLKAG
                     MTLDGQIATASGEAKWITSVPSRSDVHRLRSEVDAVLVGIGTVVSDDPSLTARLGPRF
                     KKLASEQPLRVVADSRLRIPFKAQILKRQHAAKTLIATTRSAPAAIRHALTKLGVEVV
                     VLPSLRGRVSLPALMTELGRRGVTSLLVEGGSEINAAMLQAKLVQHLRLYMAPALLGG
                     SNAKGVIGGKSPARLDETLKLKHVRTRWLGGDIVVEGDL"
     gene            7615..8901
                     /locus_tag="VFR82_10170"
     CDS             7615..8901
                     /locus_tag="VFR82_10170"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU4502006.1"
                     /translation="MSPWLAGLGILALAIGLETSLTLARVDPPESLSSLVFHYLDSAD
                     SEEAERLLQVLLNHPDTSVERVTRIIETERTYEAQPIGTVANEEVRVEGQTYAVALSI
                     PLTYRSANGYALVVCLHGAGFTGDVYLERWQARLGEEYLLACPTYPTGAWFTRRAEEV
                     VLATIEHVRRRYHVDPDRIFLTGMSNGGIGAWLIGMHHAPLFAGVVPMAGGLDDVLMP
                     FLANLRNTPIYMIHGAKDQVMPVSLSRSISRELTALGYAHVYREHQGEHPVAGGHYFP
                     REELSALRTWLDRQRRDPAPTKLTLVREASRFHPFSWVRVDATDVIAAFSDDLIDKRD
                     QRIKQREYAKLEAAIVGPNRIEVRTNHIQRYSLFLNDRLIDTAKPVSIWTNGQLSYDG
                     PVIVSIDLLLRQARLRHDPRQLFPIHLPVLVPRQAS"
     gene            8898..9707
                     /locus_tag="VFR82_10175"
     CDS             8898..9707
                     /locus_tag="VFR82_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502007.1"
                     /translation="MSIGDRLVHGNRSRCFWLILALLGSVSHTRAESCFVASEQSGIA
                     FPSEKIPGDWTCRLQFVIDHHVSASTVGPLKTAMAELLYLYLLDRPPVAAALINRLEL
                     ADYKSEERGPGRWWGSDGEGTEGIVQLVYQDRTTRVYYLEGTHHSRLLPNVSGKAVIF
                     LRMRPVKDARGSDAMESTMVAYTVLDSRVLSGFVSLLRPLVDVTVIRKLSKGVEVVNR
                     LGLEMSQRPDRVLFEATDPPPLPEQDVAFLTQVLSRRHTSSATPNSSRQAP"
     gene            9704..10870
                     /locus_tag="VFR82_10180"
     CDS             9704..10870
                     /locus_tag="VFR82_10180"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4502008.1"
                     /translation="MTTARSFTLLCTIGVFCFISYNMVRMPVLALFAESLGASPERIG
                     VIVSVSTLTGVLLKLPSGALSDIYGRRILLRVGVIAFGLPPFLYPFISDLNLLTLLRL
                     FHGLATAIFAPSALATVAELYRERRGAALGTYTACTQSGALLGPFIGGYLAFAAGFPS
                     AFLTAGFFGCIAIVLFYSLRLTPPPPRNQEKGVAPVVTEMWKGFSIVARNRKVLITSS
                     TDAAKMIANGALMAFLPLYGVGVGLNAGEVGLLFTVQSLTSFFSKPIMGRVSDRVGRQ
                     PLIMLGLVICAVTFVCIPHVASFAVLLLLTAGFGFGEAVVSSSSSAFVADSSEFKTLG
                     AGMGMQGTIGDIGHASGPLLAGVLIAHTSYASAFTTIAGLQMAAAGFFWLTMRK"
     gene            11220..11873
                     /locus_tag="VFR82_10185"
     CDS             11220..11873
                     /locus_tag="VFR82_10185"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU4502009.1"
                     /translation="MFTGIVEEMGAVTAIEKTLAGTRLTILASAVMGDLKVGDSVSIN
                     GTCLTVVTKGETTFVVDVSPETLSVTTLGELVGGMPVNLERAMKLNERIGGHLVAGHV
                     DAVGSIRSRHQEGNAIVLSIEAPPDVLRYCVSKGSVTVDGISLTINDVTDYSFSVAII
                     PHTATATTLGLKQVNDTVNLESDLIGKYVERLLQQRSQLPKTTPVIDKDYLKKRGLL"
     gene            11938..12501
                     /locus_tag="VFR82_10190"
     CDS             11938..12501
                     /locus_tag="VFR82_10190"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEU4502010.1"
                     /translation="MRWSLFPSTPPPGFHYRPDWITEEEEKPLLSQIEKLSFSEVRMH
                     GVTAKRRVVHFGWNYGYDSWTIEPTEPIPDWLMPLRLRAGALMGVSAEAVEEVLVTRY
                     EPGAGIGWHRDAPMFGPMVVGVSLSGVCRLRFQRKVEGERATAEAILAPRSSYLLAEE
                     ARFLWQHSIPPAKELRYSITFRTLQPR"
     gene            complement(12552..12764)
                     /locus_tag="VFR82_10195"
     CDS             complement(12552..12764)
                     /locus_tag="VFR82_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502011.1"
                     /translation="MRKPGEPIYLRRHVVALALAVGLPIIALQLYKIFVGPVHFVVQI
                     GLTALASVVAGLWLYVVYRSSAQNEP"
     gene            complement(12939..13415)
                     /locus_tag="VFR82_10200"
     CDS             complement(12939..13415)
                     /locus_tag="VFR82_10200"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEU4502012.1"
                     /translation="MVKRMGRMMMLIGTVGFLLVTGDPGLAPAADKEKPATKPKATPE
                     AKAPKPKAPKGDKPKYQTAEKAGAAKACFGEAPRITKLNPDEGKAGDKVTITGTNFGS
                     ADCLRGVSFGPGHAATFKVKDETTITATVPTAGRKGLAIVTVTTASGEDSKPFLMK"
     gene            complement(13467..15356)
                     /gene="acs"
                     /locus_tag="VFR82_10205"
     CDS             complement(13467..15356)
                     /gene="acs"
                     /locus_tag="VFR82_10205"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEU4502013.1"
                     /translation="MTENIETLLKESRVYRPTAKTIAAAHIKDYETDYQKSIADPEAF
                     WSGMAKELDWFSPWSTVLEWNYPWAKWFVGATCNITYNCLDRHVKTWRKNKVALIWVG
                     EDDRERIFTYGELYRQVNRCANALKKLGLNTGDRVTIYLPKIPEQLIAMLACARIGVI
                     HSVVYSGFSAPALASRINDAESRLVITADVGFDRTKVIALKPIVDEALKQCSSVERVV
                     VVRRLPSSPTPAAPKELDWTDWVKGEPAVCEAKPLDSETPLYILYTSGTTGKPKGVVH
                     VHGGYMVGTYTTTKYVFDMKDDDVYFCVADPGWVTGHSYIVYGPLLNGATVLTAEGKP
                     DFPNPGRWWDLIERYGVSIFYTTPTAIRLLMRHGEDWPKKYDLSSLRILGSVGEPINP
                     EAWEWFHRAAGGDKPIMDTWWQTETGSILITPLPTVALKPGSATRPFLGIEADVVDRQ
                     GNPLPANQGGFAVIKKPWPSMMRTIYKDPERYKTYWSIIPNCYTAGDVCHKDADGYMW
                     FMGRADDVIKVAGNRLGTAEVESALVSHHAVAEAAVIGKPHKTVGETIKAFIILKQGE
                     TANPALIESIKDQVRHELGKIAVPSEVDIVTSLPKTRSGKIMRRVLKAKELGQDPGDI
                     STIEE"
     gene            15599..16927
                     /locus_tag="VFR82_10210"
     CDS             15599..16927
                     /locus_tag="VFR82_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEU4502014.1"
                     /translation="MACLGPVAASAQMGKPEGLYYKSWAVVVGIENYLLAPKIPGVIE
                     DAKAVAEVFRKLGFEEVVELYDKDASSRRLQQTLVDFLPRKVGRQDRLVIYFSGHAGI
                     TQSGSGKDLGYLVPWDAQPGNVSKAVTFEQLKEFSHRSASKHTLFVFDTAVRGWEASA
                     AQALSLEGRSAPEDDTEKRAVQVLTAADKGEAAVRTQDRSLFVLALIKALTGEGDIDK
                     NGWLMASEVGRFVSQQVTEHTKGTQHPMFAQLEGDGDTIIIEGRKAAFRLGGEPQSPS
                     ERQQAARMQYEQAFALLQNGKAQEEALERLNKALEYDPTFGDAYVLKSYVRLEVIPDL
                     EEALKAGKLAAQYAPNNPDSHYTLGLIYEKRGQYAEAERALRAALAANPSYVDVYFTL
                     GSLYADQLKDQKKAVEAFERYLELGGTHNRAREAVAQSHAADTPPPPKNP"
     gene            complement(16924..17094)
                     /locus_tag="VFR82_10215"
     CDS             complement(16924..17094)
                     /locus_tag="VFR82_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502015.1"
                     /translation="MADVQIDDGIIKILNLDVQDPKAAAVLAEYPQVRWAEITRRALK
                     IGLGYLKGGARD"
     gene            17258..17824
                     /locus_tag="VFR82_10220"
     CDS             17258..17824
                     /locus_tag="VFR82_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502016.1"
                     /translation="MNQLRGYFSNAICSALTLILVLGLAACGGPPKWVKQGSGAFNEK
                     DTKAFYGVGSVTGVKNEPLAWDTAENRARAEIAKTFETYTGYLMRDYAASTTAGDFTR
                     NTEEQNVERAIKTVTTTTLSGVRPIERYKDEKTSTYYVLTKLNLEEMKNNLEQAKELN
                     AQVRDFVRKNADKLFERLEKEEDKRANH"
     gene            17849..18472
                     /locus_tag="VFR82_10225"
     CDS             17849..18472
                     /locus_tag="VFR82_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HEU4502017.1"
                     /translation="MNIMTQHNLWTKSAVLMGLLVGLAACGGETRVTRIDAGVVTDLS
                     GRWNDTDSRMVAESMVKEALEFPWLNTFSQSKRRQPVVVVGTVLNSSHEHINVHTFVT
                     DLERELTNSQKVTFVAGKNERDEVRSERKEQAMYAREDTQKAPGKEIGADYIMKGTIA
                     TILDEADGTKAMFYQVDLQMVDLESNAKVWFGQKKIKKVIEKKRTIF"
     gene            18546..19982
                     /locus_tag="VFR82_10230"
     CDS             18546..19982
                     /locus_tag="VFR82_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502018.1"
                     /translation="MSAQVLTFLCTVVLGGCGSSQSHYILVDKSLLTSDYQQADDLIR
                     QAEKDYGPKSRVLYGMDRGMTLQLAGEYEQSNQVLEQAEDEVDRLYTRTVRTETAAFM
                     TNDNILPYEGEPYEQVMINVLKALNYASLNRWQDALVEARRIDHRLNVLADRTANKAA
                     YRDDGFARYLSGILYESTGDVNNAFIAYRKAYETFDSSRGWSRTGVPAQLQADLLRTA
                     DALHLSQELSDYQQRFPDTVWTSLETNQQLAQVIVISYNGRAPRKEDQFLDLPISLDA
                     LQLVLLNRGIFQGNRQQYRTADTALYGLNGRVVRVALPMMVPQKTQVRTDTIRLLPAG
                     GAPLTVRTELVQNVTALASKALAERLPGITVKAVARAALKFSMAEAATRGAQQAAGKD
                     AAPLVGLAVGLLTKGFAVASEESDKRSWRTLPDEIHLARAWVSPGKYRMQGAAADVGF
                     RLIGPAPSEKTLSLQAGETVILIQRVMQ"
     gene            19979..21136
                     /locus_tag="VFR82_10235"
     CDS             19979..21136
                     /locus_tag="VFR82_10235"
                     /inference="COORDINATES: protein motif:HMM:NF014248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HEU4502019.1"
                     /translation="MNLALPYGAGHAAIVLTALLVGCSGWGGPSKPSWMDGASSEYPS
                     AQYLTGVGQGDNRAVADDQAYAALARIFKAEISAHSQDWESYLVVEQRGTSHDERRLT
                     LDQLTRVSTDKVLENAKILDRWYDAGKGLYYALGGMHRAQVETALIERMTELDRAVSS
                     DVDEAGQTSDRLAKVRALRRATRNVVLRETYNADLRVVRASGQGMPPRFRLQELSREL
                     EDYLASHLVLTVAITGDHVEPVQRALTEGLIKEGLRVAALPSGTETASGAGPRPIPEL
                     LIRGMVRVWPVDIRDPQFTYVRWCSDFDIVDLAGQQIIGAISKGGKEGHLSEREATAK
                     VIRVMQQDLSADVAKAIAAHVYGESELPAEPIRPAGCPREALKARPANATH"
     gene            21162..21425
                     /locus_tag="VFR82_10240"
     CDS             21162..21425
                     /locus_tag="VFR82_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502020.1"
                     /translation="MSRSKPGGRAPRSRGRGLTATQQRVPSRLAKRRLNERLKSDTAA
                     FNGGNLADKLRREGYEEVPLDELQDRLSKMPGPLAAVILKGRA"
     gene            21429..21872
                     /locus_tag="VFR82_10245"
     CDS             21429..21872
                     /locus_tag="VFR82_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU4502021.1"
                     /translation="MPYYYFDSTALVKRYSMERGTRIVNKLMVKRGKVAILPTWTVTE
                     LYSAFSNRAQQGELTRDDCYSVIHKFERESAQGLFQFILPTMQTYLSTKELMMEYPGL
                     RTQQVLHLALALELKPLRLTVVSADTQLLSASKTAGLHIINPEED"
     gene            complement(21896..22402)
                     /locus_tag="VFR82_10250"
     CDS             complement(21896..22402)
                     /locus_tag="VFR82_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502022.1"
                     /translation="MKLSPALIGIVTLAIACITDPAWTQTMGDEAELERLRVKAEEAI
                     ASDDPEGAAMNMGRAALMAKRLGLLNRDNHASVRLYQGAESLFRSQEHGYRAMALFRR
                     AGGQLPASSGVCGSLALAQGNVQRALLDLSSESLPVSLVERATRWRETADDWVIVVAA
                     MITDYQCP"
     gene            complement(22451..23524)
                     /locus_tag="VFR82_10255"
     CDS             complement(22451..23524)
                     /locus_tag="VFR82_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU4502023.1"
                     /translation="MRQLALSSLVLLVLLLSNSWARQESLTAEEKQKLEKIDRVLVEV
                     IAISDKGSTDAGPLTDVVVKRMKEFGYTVVTDPSQPHDVTLNVKCEQRKIWEGTTKMG
                     SDADLPDSPSRLWKGPACQLSYVLEGKKMPWRKEVRTDFTDAEQAAAVANAGDPSAFA
                     MSKLKERLEDYDFPALITAEWGQEERLFKLYDDPSTSSARKVRLIGLFGYLFETKAVP
                     RLLAGLKDKDIEIAKASALALGNIGQKDSVPTLIQAMTSGPAELRPSAAKALGVLGAL
                     HGDFTIVEPLLETLKTDDVALKTEVAWALGKLPDRKAYEPLYALQKSLHQVRDHDADP
                     KVVKLKEAVNWSIKQIDTWEYLQ"
     gene            23626..>23811
                     /locus_tag="VFR82_10260"
     CDS             23626..>23811
                     /locus_tag="VFR82_10260"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU4502024.1"
                     /translation="MKRFKERQYNRRMLSGLTVGFIGAGNMAEALISGLLHGRRIAPD
                     RLLASDINASKLARLREI"
BASE COUNT         5060 a         6541 c         7105 g         5105 t
ORIGIN      
        1 cctacccgca gccttgtcgt cgacctacgg acggccgcta cggcgaaaat ccaaaccgca
       61 tgcagcacta ctatcagtat caggtcgtgc tcaaaccatc tccggacaac attcaagagt
      121 tgtatctcga cagccttgcc cggcttggca ttgatcccaa aaagcacgac attcgatttg
      181 tccaggacga ctgggagtcg ccgacgctcg gggcctgggg attgggatgg gaagttcgtt
      241 tggatggaat ggaaattact caattcacgt atttccagga gataggtgga atcgagctga
      301 gtccgattac cggagaaatc acctacggca ccgagcgcat cgcgatgtac ctgcagcagg
      361 tcaacaacgt attcgatctg acttggacgg acggcatgag ctacggagac attcaccacg
      421 agaccgaagt gcagttctct cgctataact ttgagcaagg cgatgtcgcg atgttgatga
      481 acgccttcca atcatacgaa gccgaatgta aacggttact cagccagagc gatcagcggt
      541 tgacgttgcc ggcgtatgac tactgtataa aatcttcgca tgccttcaat ctgttggatg
      601 cgcggggagc gatcagtgtc gccgagcgca ccggctatat cgcccgcgtc cgggcgttgg
      661 cccggcaatg tgccgagcgg tatatcgagg agcgggcctc aatgggccat ccgctcatca
      721 aacaccatca ggcggctcgt gcggggaggc ggccgacagc cggggtgggc cggagcaccc
      781 gacgatgacg cgccgaaaag cgccaacggg tcggttgcag gtgtcttccg ccagcggcgc
      841 cgagctcttg ttcgaaatcg gcgtcgagga acttccatac caagtgattg ctcccgcgat
      901 gtcggcactg aaagaggtcg ccggccgtct tctcgaagaa gccaggctga cgatcgagtc
      961 ggtcaatgtg tacggcaccc ctcggagatt gaccctcgtc atgagcgggc ttgcgcttcg
     1021 tcagacgtcc gccaagaaag aagtgatggg tccttcaaaa gttgtggggt ttgattcgca
     1081 ggggcaacca acgaaagccg caattggatt cgccgctgga caaggcttgt ctgtcgagcg
     1141 cctcgaggta cggcgaaccg ctaaaggtga ctatctgttc gcggtgaaag aggaggcagg
     1201 gcgaccgacg caagccgtgt tgttagaact ccttcctcaa ctgatgggta agctctcatt
     1261 tccgaaggcg atgaaatgga atgaaactgc caccaggttt gccaggccag tgcgttggct
     1321 catcgctctg ttcaatgggg ccctcttgcc gatcgaagcg gcagggatac gagctggcga
     1381 ccggacctat gggcaccggg tgatggggca gcgcaagccg ctcgtggtgc gaaactatga
     1441 gtcctatcgc agcggtctgg aacgggcggg tgtgctcgtc gatccggtga cccggcgaca
     1501 agtcatcgag cagcagctcc accagatctg ctccagagcc gcagtcgagc tcaacgccga
     1561 cgaatcgctg atggatcagg ccgtttttac gaccgaatgg ccgtgcgccc tgctaggcag
     1621 ttttaaaccg gaatatctca gcctgccgga ggaaattttg atgacctcta tgaaagagca
     1681 tcagggattc ttctcggtgc gggacaaaac aagtgggaag ctcgcgccgc attttctagc
     1741 ggtggcgaac aaccggccta aagatatgtc gttgattcga gcaggaaacg agcgtgtgct
     1801 cgctgcccgg ctggccgatg ccaagttttt tttcgacgag gatcgcagaa cacgcttgga
     1861 agacagggtg aaaaagctca gcggtgtcac cttccatcaa aagctgggca cgatgggaca
     1921 gaaggcggaa cgcctggtcc aactggtggc cctcatagcc tcaggcatcc atcgcgactc
     1981 ggagagtcaa gactttgtga atgcatgccg aagagcggct catcttagta aagcggatct
     2041 cctcaccggc atcgtaggcg aatttcccga gcttcaaggc atcatgggtg gcgagtatgc
     2101 caagcatgat ggagagccgg aagccgtgag tcaggcgatt cgcgagcagt acctgccgaa
     2161 ggcgctggaa ggggaattgc cgcgatctct tcccggactg gtcttgtcgc ttgcggaccg
     2221 attggacacc accgccgcat tttttcatgt cggcatggtt ccaaccggct cggaagatcc
     2281 ttttgccctt cggcgtcacg caaccgcgat cgtgcgcatt gttctcgaaa aagagcttcg
     2341 cctggacctt gggaaataca tcgattacgc acggacactc cttgttgaca gtggcatcaa
     2401 gggagctccc gattccgaac agcacggacg tcggcgcgta actgattttg tcttcgagcg
     2461 cgtgcggcat tacgggcgca cagtccacgc gttccgcgac gacgttatgg atgccgtctt
     2521 gcggcaggtt tccgccggac cgatcgatct ggtcgacctc ctgctaaagc tgaaagcggt
     2581 gcaaggggtg acggccaagg cggatttcga cccactcatc gtcggtttta agcgcgcctc
     2641 tcggatcatt gagaaagaac agtgggatcg taagccggtc gatcctggaa tgttccagga
     2701 agcgggtgaa catgaattgc atcggcagat ccgtgcctgt cagcatgact tccaacagca
     2761 tatggcggag ggctgctacg acagggctct ggacgctctc gtccggctca aaccggcgat
     2821 cgatgcgttc tttgaaggcg tcatggtgaa tgtcgaagat gcggcactga gaagcaaccg
     2881 gttatcgctc ttgaaagacg tcagcgactg ttttacgtcg ttcgcggact tttctcggat
     2941 tgtggtacaa ggaggctaga agaaggcaaa cgcccggtgg tctccttcgc tcaccggaac
     3001 cccaggtgaa gtgcggccct tcgctggcgc aggtggacac cagccggctc ctcccgctgg
     3061 aagatgaagg tagaggatga tcgcttcatc tgagtacgac cttgaagaga agcgtgcggc
     3121 gaaaatccga cggtttgcca gcgtggtcac ggtcgggctg atcgtcttct gcaatgtcct
     3181 gctcttgttc ggagtctggg tgagcggcgt caacctcgac gaattggtga agacgcccga
     3241 tctgttcgat gcgaaagagg atgtctgcct ccggctgagt tgggagcgtg tctccggagc
     3301 ggcggatccg gtgcgactgt gttccgaatg gatcaaactg gctgatccat ccggtaaacc
     3361 gcatcagttg gccaaggaca tcgccgtccg gcgagggcct gacggccaat attacgtgga
     3421 tcgaggcgtc aggccggact ttcgtttgct gggattggcc ctgttcgtgg gtgtggtgtt
     3481 gttgttcggc atgtgggcga gacgatatct cgtgagccgc tatcggcttc agattgggcc
     3541 ggcggactcc cgcagctcgt cgggccttca ctgacaggtg aaggaaggag agagatcgtg
     3601 gctaagaaat acgtgtatta cttcggagac ggaaaggctg aaggcacctc caatatgaag
     3661 gagttgctgg ggggcaaagg ggcgggattg gctgagatga ccaacctggg catctcggtt
     3721 ccccccggtt tcaccatttc aacggaagcc tgtgtcgagt attacaagct cggcaagaaa
     3781 tatccgcccg gcatgtggga agcgtccctg caagcgctca agcgtgtgga acggtcaatg
     3841 ggcatgggtt ttggcgatcc cgaacggccg ctgctggtct cagtgcggtc gggagcccgt
     3901 gcgtcgatgc cgggcatgat ggatacagtc ttgaacgtcg gactgacgac gaagacggtc
     3961 gaaggtttgg ctgccaagac caggaacgat cggttcgcgc aggatagcta ccgccggttc
     4021 gtgtccatgt tcggcagcat cgtgatgggg gtgcatcgtg aacatttcga agccattttg
     4081 aaacacaaga aagccgaagt gggagtgacg catgaaaccc atctcgacgc ccgccatctg
     4141 cgcgagttag tcgccagctt caaggcgctg gtcaaggaag agacgaagaa agaatttccc
     4201 gacgaaccgc tcgatcagct gcgcatggcc atcagtgccg ttttctcgtc ctggttcggc
     4261 gcccgagcga tcacttatcg acggctctat ggtattccgg attcctgggg aacggcggtc
     4321 aatgtggtcg cgatggtgtt cggcaatatg ggagaaacca gtggaaccgg ggtggcgttt
     4381 acgcgcagcc caagctcagg ggacaaagcc ttttttggcg aatgccttat gaatgcgcag
     4441 ggggaagatg tcgttgccgg tatcagaaca ccgttgcccg tcaacgcatt ggccaaaaac
     4501 gttccggagg cctatcggga actcgagcag acctacaaaa agctcgagaa gcactaccgc
     4561 gacatgctcg acctggagtt cacgatccag gagggcaagc tctacatgct gcagacccgc
     4621 gttggaaagc gcaccgggat cgccgccgtc aagattgcgg tagacatggt gaaggaaggc
     4681 gccatttcca gggaagaagc tgttctacgc gtggggccgg atcagttggc gcagtatatg
     4741 tatcccattt tcgacatgaa ggaagagtcg aaggcgaatc cgcttggcaa gggcttgcct
     4801 gcaggccctg gcgcggcggc agggaaaatc gccctgacgc ccgatcgcgc cgttgatatg
     4861 aaggctgccg gcagtcgggt cgtgttggta cgccaggaaa caagtccgga cgatattcac
     4921 ggcatgaatg cggccggcgg cttcttgacc gcccgtggcg gtatgacgtc tcacgccgcg
     4981 gtcgtggcgc gccagatggg taaggtctgc gtcgcagggt gtgactcggt ggaagtgctc
     5041 gacagtcaaa ctgtgaagat cggggcgaaa acgttccgag aaggcgacta tctctccatc
     5101 aacggctcca cggggaacgt ctatgaaggg gatattccgg ttgttgattc cgaaatcatt
     5161 caagtgatcc aaggcaagtt ggatcccaag aaatccgaaa aatatcagcg cttctccacg
     5221 atcctctcct gggcagacag ccttcgccgc ttgcgcgtgc gggccaacgc ggatgtgccg
     5281 gagcaggcga agattgcccg cggcttcgga gcggaaggaa tcggactatg ccggactgag
     5341 cacatgtttt ttgccgaaga ccggattccc cttatgcaga aaatgattct ggcccggacg
     5401 acggaggagc gggaaaagta tctggatcaa ttgcggccgc tccaaaaaca ggatttcatc
     5461 ggcctgtacc gtgagatgga aggttttccg gtgacgattc ggttgctcga tccaccgctc
     5521 catgagtttc tccccaagcg ggaagacctg atggtggaaa ttgctcagct ggaactgacc
     5581 ggtaaggacg ccgcgaagct ggaggaaaac cgccgactcc tggcgcgtgt ggaggagttg
     5641 cacgagttca atcccatgtt gggtttgcga ggctgccgcc ttggcattac gatgccggag
     5701 attacgcgca tgcaggcgcg ggccgtcatc gaagcggcct gcgaattggc caaggaggga
     5761 aagaaaattg tgcccgagat catgattcct ttggtgggaa tggtttcaga aatgaaatct
     5821 caaaaggatc tcgttcgtga agtcgctcag gagacgatga agcgttacaa cgtcaaactg
     5881 tcctacctgg ttggaacgat gatcgagctg ccgcgggccg ccgtgacagc tgaccggatc
     5941 gccgaagagg ccgaattttt ctcgttcggt acgaacgacc tgactcaaac gacattcgga
     6001 ttctcacgtg acgacgcggc caagtttatc gatcactaca agaccgcgaa tctcatggaa
     6061 tccgatcctt tcgcggtgct ggaccgtgag ggtgtcggct gcctcatgag tcaggccatt
     6121 gcgggtggcc gcaagacgcg cacgggcatc aagcttggca tctgcggtga acatggcggg
     6181 gaccccagtt ccgtcgagtt ctgccaccag ctagggttgg attatgtcag ttgttcgcca
     6241 tatcgtgtcg ccatcgcgcg cctcgctgca gcacacgctg ccttggctga ggcagatgcc
     6301 cgcaaagccg ccaaggcgac gctccgaaag accacgaggc gaaagcagtc gaccgcgagg
     6361 cgaaagcagc gatagtcgac cgacatggca ctatgaaggt gtctcccacc acggcgtcta
     6421 gacttctcaa cgatcggctt cctgatgtgg ctggtgcttt tccgatcttg cgttgtgtga
     6481 cgaggcctcg tggataatgc gggctggcca cgcacaggat cttcattata tgaaattcgc
     6541 gctcgccctc gcggccaacg gccgaggtac ggcgagcccc aatccgatgg tcggggccgt
     6601 cgtcgtgagc catggacgga ttgtcggcaa gggatatcat ctccggcccg gtcttcccca
     6661 cgcagaaatt ctggcgctga aagcggcggg gcctcaggcc cgtggcggca ccctgttcgt
     6721 gacgctcgaa ccctgctgtc atctcaataa gcggacgcct ccctgtgtgc cggccgtcat
     6781 atgggcgggc gtgacacgcg tggtgattgc tcagcgcgat cccaatcctc tggtcaacgg
     6841 taaagctctg aaggcgcttc ggcgcgccgg agtatccgtt acggtcggcg tgggaaagtc
     6901 cgaggcgatt gagttgaatc gtgcgtataa tcactacatg cgtacgaggc gtccttatgt
     6961 gcttctcaaa gccggaatga cactcgacgg acagattgca acggcttctg gcgaggcgaa
     7021 gtggattacg agcgtgccgt cgcgaagcga cgtccaccgg cttcgttcgg aagtcgatgc
     7081 ggtattggtc ggaatcggga ctgttgtgag cgacgaccca tcgttgaccg cccggctcgg
     7141 tccgcgcttc aagaaattgg cgtcggagca accgctgcgc gtcgtcgctg acagtcgact
     7201 ccggattccc ttcaaagcgc agattctcaa gcggcaacat gctgccaaga cgctcattgc
     7261 gaccactcgc agcgctcctg cggcgattag gcatgccctc accaagctcg gagtggaggt
     7321 cgtcgtcctg ccgagtcttc gaggacgtgt ctccctgccc gccttgatga cggaactggg
     7381 gaggcgggga gtgacgtcgt tgctcgtgga gggtgggagt gaaatcaatg ccgccatgtt
     7441 gcaggccaag ctggtccagc accttcgcct gtacatggcg cctgcactgc ttggtggcag
     7501 caacgcaaag ggtgtgatcg gagggaaaag tccagcccgt ctcgacgaga ccttgaagct
     7561 caaacatgtg cgtacccgat ggctcggcgg cgacatcgtg gtcgaaggtg atctatgagt
     7621 ccatggctgg ccggcttagg cattcttgcg ctggctatcg gcttggaaac gagcttgacg
     7681 ctggctcgag tggatccgcc ggaaagtctc tcttcgcttg tctttcatta tctcgattca
     7741 gcagacagcg aagaggctga acggctcttg caagtgttgc tcaaccatcc tgatacctct
     7801 gtcgagcgcg taacccggat cattgaaacc gaacgcacct atgaggctca acccattgga
     7861 acggtagcca acgaagaggt acgagttgag ggccaaacct acgcagtggc actgtcgatt
     7921 ccgttgacgt atcgatccgc gaatgggtac gccctcgtcg tgtgcctgca cggcgccgga
     7981 tttaccggcg acgtctatct ggaacgttgg caggcacggc ttggcgaaga gtatcttctg
     8041 gcttgtccga cgtatccaac cggagcctgg tttacgcggc gtgcagagga agtcgtgctg
     8101 gcgacgatcg agcacgtccg ccggcgttat catgttgacc ccgatcgaat ttttcttaca
     8161 ggcatgtcga atggggggat cggcgcctgg ctcattggca tgcaccatgc gccgctcttt
     8221 gccggagttg tgccgatggc cggcgggctt gatgatgtgc tcatgccgtt cctcgcgaat
     8281 cttcgcaata cccccatcta catgattcat ggggcgaaag atcaagtgat gccggtgtca
     8341 ttaagccggt cgatctcacg cgaactcacg gcattggggt acgcacatgt ctaccgcgag
     8401 caccagggag agcatccggt cgcaggcggc cactattttc ccagagagga gttgtcggcg
     8461 ttgaggacat ggttggaccg tcagcgccgt gaccctgcgc cgacgaaatt gacgctcgtg
     8521 cgcgaagcca gccgcttcca tcctttcagc tgggtgcgcg tcgacgccac ggacgtgatc
     8581 gcagcgtttt cggatgatct gatcgacaag cgcgaccagc gaatcaaaca acgcgaatac
     8641 gcgaagcttg aagcggccat cgtagggccg aatcgtatcg aggtgaggac gaaccacata
     8701 cagcgctata gtctcttttt gaacgatcgc cttatcgata cggcaaaacc ggtcagcatc
     8761 tggacgaacg gacaactctc ctatgatggt cccgtgatag tgtcaattga cctactgtta
     8821 cgtcaggcac gactccgtca cgatccccgg cagctgtttc ccattcatct tccggtgttg
     8881 gttccgagac aggcctcatg agcatcgggg accggttggt acatggaaac cggtcaagat
     8941 gtttctggct cattcttgcg ttgctcggat cggtttctca tacgcgggcc gagtcctgtt
     9001 ttgtggccag tgaacagtcg ggaatcgcat ttccgtctga gaagattccc ggtgactgga
     9061 cttgccgcct ccaattcgtc atcgatcacc acgtcagtgc cagcaccgtt ggcccgttga
     9121 aaaccgcgat ggctgagcta ctgtatctgt acctgctcga ccggccaccg gtagcggcgg
     9181 ccctcatcaa tcggctggag ctggcagact acaagtccga ggagcggggt cctgggcggt
     9241 ggtggggcag cgatggggaa ggaacggagg gcatcgtgca gctggtatac caggatcgca
     9301 cgacgcgggt atattacctt gaagggacgc atcatagccg cttgcttccc aatgtaagcg
     9361 gcaaggccgt gatctttctt cggatgagac cggtgaagga cgcgcgcggc tccgacgcaa
     9421 tggaaagcac tatggtggcg tacacggtgt tggatagccg tgttctttcc ggcttcgtgt
     9481 cgcttctgcg cccgctcgtc gatgtaacgg tgatacgcaa attgtctaaa ggcgtcgaag
     9541 tggtgaaccg gttgggtctc gaaatgagcc aacgccctga ccgcgtcctg ttcgaggcca
     9601 cagatcctcc tcccttgcct gagcaggacg tggctttctt aacgcaggtt ttaagccgcc
     9661 ggcacacttc ctcggccact ccaaactcct cgcgtcaggc gccatgacga ccgctcgcag
     9721 ttttacgctc ctctgcacga tcggcgtgtt ctgttttatc agctataaca tggtacggat
     9781 gccggtcctg gcgttgttcg ctgagtcgct cggtgcaagc cccgagcgaa tcggcgtgat
     9841 cgtgtcagtg tccactctga caggcgtgtt gctcaagctt ccctccggcg ctctttcaga
     9901 catttacggc cgtcggatac tgctgcgcgt cggcgtcatc gcgttcggtc tacccccgtt
     9961 tctctatccc ttcatctctg atttgaatct gctcacattg ctacggctct ttcacggctt
    10021 ggcgacggcg atcttcgccc ccagcgcgct cgctaccgtc gccgaactgt atcgcgagcg
    10081 gcgcggggca gcgctcggca cctatacggc ctgtacacag tccggagcct tgttgggccc
    10141 gttcatcggc ggatacctcg cctttgcggc cggctttccc tcagcgtttc tcactgccgg
    10201 atttttcggg tgcatcgcta tcgtcctatt ctatagtttg cggctgaccc cgccgcctcc
    10261 acgcaatcag gaaaagggcg tggcgccggt ggtgacggaa atgtggaagg ggttcagtat
    10321 tgtggctcgc aaccgcaaag tcttgattac cagttctacg gatgcggcca aaatgatcgc
    10381 caacggagcg ttgatggcct ttttgcctct ctacggcgtt ggagtcggcc tcaacgctgg
    10441 cgaagtcgga ctcctattca cggtgcaatc cctgacgtcg ttcttctcaa aacccatcat
    10501 gggccgagtc tcagatcgag tgggcaggca gccattgatc atgctgggct tggtgatttg
    10561 tgccgtcacg ttcgtctgta ttccgcatgt ggcgagtttt gccgtgttgt tgcttctgac
    10621 ggccgggttt ggtttcgggg aggcggtggt ctcgtcttct tcctcggcct tcgtggcgga
    10681 cagctcggag ttcaagacat tgggcgcagg catggggatg caaggaacca ttggtgatat
    10741 cggccacgcc agtggtccgt tgctggcggg tgtgctcatt gcccacacga gttatgcctc
    10801 ggcgtttacg accattgccg gcctccagat ggctgccgcc ggattctttt ggctcaccat
    10861 gagaaaataa ataccgctat aatggagcgc catgcgagaa atcgcgactg aaaacattgt
    10921 gatcggtgtg cttgcgtcga tcggcgtcat ggtcctgatc gttgtgtttg tctatgtgtt
    10981 gaagaaactt tttggtcaga aagccgaata ggctttcctg gttcaccttc tcacaaatag
    11041 ccaggacgag aagcgggaat gaagcgggcg atagacgcgt tcctgggaat cgattgcaga
    11101 gtagtcagtg gggcgagaca aaggaatcgc cggggttggg cgggttagac tgggcagtcg
    11161 cggcgtctcg gcgccgcggg gctgggcggc tgagaaaggg ccattttcta aatcttgcta
    11221 tgttcacagg aatcgtcgaa gagatgggtg cggtgaccgc gatcgaaaaa actctcgccg
    11281 gcacacgact gacgatcctg gcttcggctg tcatggggga tctgaaggtc ggggacagtg
    11341 tcagcattaa cgggacctgt ctgacagtgg tgaccaaagg ggaaacaact ttcgttgtgg
    11401 acgtgtcccc agagaccttg tccgtgacga cccttggtga gctagtgggg ggtatgccgg
    11461 tcaatctcga gcgggccatg aagttgaatg aacggatcgg cggtcacctt gtggcaggtc
    11521 atgtggacgc ggtcgggagt atccgcagcc ggcatcagga agggaatgca atcgtcttgt
    11581 ccatcgaagc gccgcctgat gtgttgcgct attgcgtatc gaagggttcg gtcacagtcg
    11641 atgggatcag cctcacaatt aacgatgtga ccgactattc tttcagcgtc gccatcattc
    11701 cccacacggc cacagcgacg acgctgggat tgaagcaagt caatgacacc gtcaacctgg
    11761 aatcagacct catcggcaaa tacgtcgagc ggttgcttca gcaaagaagc cagcttccga
    11821 agactactcc tgtgatcgac aaggactacc tgaaaaagcg cggactcctg taggagtctg
    11881 ggtcttttgg tgtccccgcc cataccactt ccacctgtag gctcgataca atggttcatg
    11941 cgctggtcct tattcccttc cacaccaccg ccagggtttc actaccggcc tgactggatc
    12001 acagaggaag aggagaagcc gcttctgtcg cagatcgaaa agttgtcgtt ctcggaggtc
    12061 cgcatgcatg gcgtgaccgc gaagcgacgt gttgtccatt tcggatggaa ttacggctac
    12121 gattcgtgga cgatcgagcc gaccgagccg ataccggatt ggctgatgcc tttgcgcctg
    12181 cgggcgggtg ctctgatggg cgtctcagca gaagcggtgg aagaagtatt agtcactcgc
    12241 tatgagccgg gtgccggcat cggatggcat cgagatgccc cgatgttcgg tccgatggtc
    12301 gtcggcgtct ctctgtcagg ggtttgccgt ctacgttttc agcgcaaggt cgaaggtgaa
    12361 cgagcaacgg ccgaggcaat cctcgcgcca aggtcgtcat atctcttagc cgaggaagcc
    12421 cgtttcctgt ggcagcattc cataccgccc gccaaggagt tgcgctactc cattaccttt
    12481 cgcaccctgc agcctcgcta agccggggtg gtgtacacgc catttcatgg gcctagcgtt
    12541 ttggccatca ttcatggttc attctgtgcc gacgaccggt agacaacgta cagccagaga
    12601 ccggccacca ctgaagccaa ggctgtcagg ccaatctgga cgacgaagtg aacgggtccc
    12661 acaaagatct tgtagagctg taaggcaata atcggcagac caaccgctaa ggccagagcg
    12721 accacgtgcc gtctcagata aatgggttcg cccggctttc tcattcctcc tcgcacgatg
    12781 gtcgtctctc actcttgctt tcggctccac ccaggggcgg gatggattga gtctccattc
    12841 ctggtattga ccgagcacag cccgtaatct aaaacctgat aagtcttgtg tgtcgcggtc
    12901 tgcgagcagg gagactcaac cagatcgtcc tctcccactc atttcatcag aaacggtttt
    12961 gaatcctctc ctgatgccgt cgtcaccgtg acgatagcca gcccttttcg ccctgcagtg
    13021 ggaacagtgg cggtgatcgt ggtctcgtcc ttcactttga aagtcgccgc atgcccggga
    13081 ccaaaagaga cgccgcgtag acaatcggcc gaaccaaaat tcgtcccggt aatcgtgact
    13141 ttgtctccgg ctttcccttc gtccgggttc agtttggtaa ttctgggtgc ttcgccgaaa
    13201 caggctttcg ctgctcctgc tttctcagcc gtttgatatt tcggtttatc gcccttgggg
    13261 gctttgggtt tgggtgcctt ggcttcgggc gttgctttgg gcttcgtggc cggtttttcc
    13321 ttgtccgctg cgggtgccag cccagggtca ccagttacga gcaggaaacc gactgtacca
    13381 atcaacatca tcatacggcc cattcgcttc accatgcctg cctccttcgc cgcagcgcca
    13441 ccggcgctgc atggactttc ggtgcattat tcctcaatgg ttgagatgtc tcctgggtct
    13501 tgacccagtt ctttcgcttt tagcactcgg cgcatgatct tgccggaacg cgtcttcggc
    13561 agcgacgtga caatgtccac ttccgaggga acggcgattt tccccagttc atgccggacc
    13621 tgatctttta tggactcgat gagcgcaggg tttgcggttt caccctgctt gaggatgatg
    13681 aacgccttga tcgtctcgcc gacggtttta tggggtttcc cgatgacggc ggcttcagcc
    13741 acggcatggt ggctgacgag ggcgctctcg acctcagcgg ttcccaggcg attgcctgcg
    13801 accttgatca catcgtcggc acggcccatg aaccacatgt acccatcagc gtccttgtga
    13861 cagacgtcgc ccgctgtata gcagtttgga atgatgctcc aataggtttt ataccgctcg
    13921 ggatctttat agatcgtacg catcattgat ggccacggct tcttaatcac ggcgaaaccg
    13981 ccctggtttg cgggcaaagg attgccttgg cggtcgacca cgtcggcttc gatgcctaag
    14041 aatggtctcg tggcggaccc aggcttcaag gcgacggtag gcagcggcgt gatcagaatg
    14101 gatcccgttt ccgtctgcca ccaggtatcc ataatcggct tatcgccgcc tgcggctcga
    14161 tgaaaccatt cccacgcctc gggattaatc ggttcgccga cgctgccaag aatgcgcaag
    14221 ctggacagat cgtatttctt tggccagtcc tcgccatggc gcatcagcag tcgaatggca
    14281 gtcggcgtcg tgtaaaagat cgacacgcca tagcgttcga tcaggtccca ccaccggcct
    14341 ggattgggaa aatccggttt cccttcggcg gtgagcacgg tggctccatt taacagcggg
    14401 ccataaacga tatagctgtg gccggtgacc catccgggat cagcgacgca gaagtacacg
    14461 tcgtcgtctt tcatgtcgaa cacgtacttc gtcgtcgtgt aggtgccgac catgtacccg
    14521 ccgtggacgt gcacgacccc tttgggtttt cctgtcgtgc cggacgtgta cagaatgtac
    14581 aacggagttt ctgaatcaag tggcttcgct tcgcagacag ccggttctcc cttgacccaa
    14641 tccgtccagt cgagttcttt cggagccgct ggggtggggc ttgacggtag tcgccggacg
    14701 acgacgaccc gctcgaccga ggagcactgt ttcaaagcct cgtcaacgat cggtttcagg
    14761 gcaatgacct tggtccggtc gaatccgacg tccgctgtga tgaccaatct ggattcggcg
    14821 tcgttgatgc gactggccag agcgggcgcg ctgaatcccg aatacacgac gctgtggatc
    14881 accccgatgc gggcgcacgc caacatggca atgagctgtt ccggaatttt cgggagatag
    14941 atggtgacac ggtcgcctgt gttcaggccc agctttttca gcgcgttggc gcatcgattg
    15001 acctggcgat agagttcgcc gtaggtaaag atccgttcac ggtcgtcttc gccgacccag
    15061 atgagtgcaa ctttattctt gcgccaggtt ttgacgtgcc ggtcgaggca attgtacgtg
    15121 atattacacg tggctcccac gaaccatttg gcccaggggt agttccattc cagaactgtg
    15181 gaccagggcg aaaaccagtc caactccttc gccatgccgc tccaaaaggc ttcaggatcg
    15241 gcaatcgact tctggtaatc cgtctcgtag tccttgatgt gcgccgcggc gatggtcttg
    15301 gccgtcggcc gatagacacg gctctctttg aggagggttt caatgttctc cgtcatggct
    15361 gaacagctcc tttgggatgg accgtaccgg atgctgccat tatcggcaag aggttggggc
    15421 ggcgcaacca tacaacggta cggtcgccga tgcaattcct tagggcccgt ggttgacagc
    15481 cgctcagggc cagagtacgc tgatttcata gagggcttgt gacccgtcca ctatgatgct
    15541 tccctcctca cgcccatcgc tccagtcacg atttctcact atcctggtcg ttctcgctat
    15601 ggcatgcctt ggccccgtgg cggcgtccgc tcagatggga aagcctgagg ggctgtatta
    15661 caaatcgtgg gccgtcgtcg ttggaattga aaactatttg cttgcaccga aaattcccgg
    15721 tgtcatcgaa gacgccaaag ccgttgccga agtctttcgc aaactaggat tcgaagaggt
    15781 cgtcgaactc tacgacaagg acgcgagttc gcggcggttg cagcagacgc tggtggattt
    15841 tctgcctcgg aaggtgggcc gccaggatcg tctcgttatt tatttctccg gccatgccgg
    15901 gattacccag agtggctcgg ggaaggatct gggttatctc gtgccctggg acgcgcagcc
    15961 cgggaatgtc tcgaaggccg taacatttga acagctcaaa gagtttagtc accgtagcgc
    16021 ctcgaagcac acgctgttcg tattcgatac cgccgtccga gggtgggagg ccagtgcggc
    16081 acaggcgctt tcgcttgaag ggcgaagcgc cccagaggac gatacggaga aacgcgccgt
    16141 ccaggtcttg acagcggccg acaaaggaga ggccgcggtg agaacgcagg atcgcagttt
    16201 gttcgtactg gcgctgatca aagcgctcac cggggagggg gacatagata aaaatggttg
    16261 gctgatggcg tccgaagtcg ggcggttcgt cagtcagcag gtgacggagc acacgaaggg
    16321 tacccagcat ccgatgtttg cccagctgga aggcgatggc gataccatta tcatcgaagg
    16381 acgaaaggcg gcgttccgcc tgggcggcga gccccagtca ccttctgagc gtcagcaagc
    16441 agccaggatg caatacgagc aggcctttgc cctcctgcaa aatggaaaag cgcaggaaga
    16501 agcattggag cgcctcaaca aggcgttgga atacgatccg acatttggcg acgcctacgt
    16561 gctcaaaagc tacgtgcggc tcgaggtcat tcccgatctg gaggaagcgc tgaaggctgg
    16621 gaagttggcg gcgcagtatg caccaaacaa cccggattcc cactatacgt tgggattgat
    16681 ctacgagaaa cgcgggcagt atgcggaggc cgaacgagcc ttgcgcgcag cgttggccgc
    16741 caacccgagt tatgtcgacg tctatttcac gcttggctcg ctgtatgccg atcagttgaa
    16801 ggatcaaaag aaagcggtcg aagcgttcga gcggtacctt gaactggggg ggacccataa
    16861 ccgggcgcgg gaagccgtcg cgcagtcgca tgcagctgac acgccgcctc ctcccaaaaa
    16921 cccctaatct cgtgcgcctc ccttgagata gcccaaaccg attttcaagg cccgtctggt
    16981 gatttccgcc caccggacct gcggatattc ggcaagcaca gcggcagctt tcggatcttg
    17041 cacatcgaga ttcaaaatct tgatgatgcc gtcgtcgatc tgcacatcag ccacggattc
    17101 ctcctttcac atattgaacc tagggacctg tcgcgttgac gggatggaag gtgcatggta
    17161 tcataaaact gcgcttgcct cgaacttctg acgcatgtac acccacgttg acccgaccgc
    17221 catctacgct acccaagcta cgcaaggagg acatcgcatg aatcagctac gagggtattt
    17281 ctccaacgcg atctgttctg cgttgacctt gatactggtg ctcgggctag ccgcatgcgg
    17341 cggacctccc aaatgggtga agcaaggatc gggtgccttc aacgagaaag atacgaaagc
    17401 cttctatggc gtcggatccg tcaccggcgt caagaatgag cccttggcct gggacacggc
    17461 ggagaaccgc gcacgggccg aaattgccaa gacctttgaa acgtacaccg gctatctgat
    17521 gcgtgattat gcggcgtcca cgaccgccgg tgattttaca cgcaacacgg aagaacaaaa
    17581 cgtcgaacga gccatcaaga ccgtcaccac caccaccttg agcggggtgc gtccaattga
    17641 gcggtataag gacgagaaga ccagcacata ctacgtgttg accaaattga atctcgagga
    17701 aatgaagaac aatcttgagc aggccaagga gttgaacgcc caagtgaggg atttcgtccg
    17761 taaaaatgcg gacaagttgt tcgagcgttt ggagaaggaa gaagacaagc gggccaacca
    17821 ttaagcagta cgcatgaagg agcccgtgat gaacatcatg acgcagcaca acctttggac
    17881 caagtcggcg gtgctcatgg gcctgctggt gggtttggcg gcgtgtgggg gtgaaacccg
    17941 ggtgactcgt atcgatgctg gggttgtgac cgacttaagc gggcggtgga acgatacgga
    18001 ctcccgcatg gtggccgaat ccatggtcaa agaagcgctc gagtttccgt ggctgaatac
    18061 cttcagtcag tccaaacgcc ggcaaccggt ggtcgtcgtc ggcaccgtat tgaacagtag
    18121 tcatgaacat atcaacgtgc acacgttcgt gaccgatttg gagcgtgagt tgaccaattc
    18181 ccaaaaagtc acgttcgtgg cgggcaagaa tgagcgggac gaggtccgat cggagcgcaa
    18241 agaacaagcg atgtatgcca gagaagatac gcagaaggca ccaggcaagg aaatcggtgc
    18301 cgattacatc atgaaaggca caatcgccac gattctggac gaagcagacg gcaccaaggc
    18361 gatgttctat caggtcgatc tccagatggt ggatttggaa agcaatgcca aggtgtggtt
    18421 cgggcaaaag aagatcaaga aagtcatcga gaaaaagcga acaattttct aaggctccgc
    18481 gaactccgtt gagccctctt gtctcacgcc ccaccggtca cgcctctccg cggtggggcg
    18541 tttgtgtgtc tgctcaggta ctgaccttcc tatgcactgt cgtgcttggc ggctgcggct
    18601 catcccagag ccattacatc ctcgtcgata aaagtcttct caccagcgat taccagcaag
    18661 cggacgatct catccggcag gcagagaagg actatggccc gaagagccgt gtgctttatg
    18721 gcatggaccg gggcatgacc ctgcagctgg ccggcgaata cgaacagagc aaccaagtac
    18781 tggagcaagc tgaagatgaa gtggatcgtc tgtatacgag aacggttcgt acggaaaccg
    18841 ctgcgttcat gaccaacgat aatatcttgc cgtatgaggg tgaaccatat gaacaagtga
    18901 tgatcaatgt gctcaaggcc ttgaactatg cttcgttgaa ccgctggcag gatgcgctgg
    18961 ttgaggcccg ccgtatcgac catcggctga atgtcctcgc cgacagaacc gccaacaagg
    19021 cagcctaccg tgacgacgga tttgcacggt acctcagcgg tatcctctac gagagcaccg
    19081 gagacgtcaa taatgcgttc atcgcctacc gcaaggcgta tgagacgttt gactcatctc
    19141 gcggatggtc acgcacagga gtgccggctc aattgcaggc tgatctgttg cgaacggcgg
    19201 acgccctgca tttgagccag gaattatcag actatcagca gcggtttcca gatacggttt
    19261 ggaccagcct cgagaccaac caacaactcg ctcaagtgat tgtgatcagc tataatggcc
    19321 gcgccccgcg taaggaggac cagttccttg acctaccgat cagtcttgac gcgctccaac
    19381 tggtgctgct caatcggggg atcttccaag ggaaccgtca gcaatatcga acggctgata
    19441 ccgccttata tgggctcaac ggccgggtcg ttcgtgtggc gttaccgatg atggtcccgc
    19501 aaaaaacgca agtgcggaca gacacaatcc gactgctacc ggctgggggc gctccactga
    19561 cggtgcgaac agagttggtg cagaatgtca cggcactagc cagtaaggcg ttggctgagc
    19621 gcctccctgg catcacggta aaggccgtgg cccgtgccgc gttgaagttc agcatggccg
    19681 aagctgctac gaggggcgcg cagcaggcag cgggaaaaga tgccgcacct ttggtcggac
    19741 tggccgttgg cctcctgact aagggatttg ctgtggcgtc cgaagaatcg gacaagcgca
    19801 gctggcgtac actgccggat gagattcacc tcgcgcgggc ctgggtgtct cccgggaaat
    19861 atcgcatgca aggtgcagct gcggatgtgg gttttcgatt gatagggcca gctccatccg
    19921 agaaaacact gtcgcttcag gcaggggaaa cggtcatcct catccaacgg gtgatgcagt
    19981 gaatttggcc ttaccatatg gggctggcca cgcagcgatc gtgctcacgg cgctgctcgt
    20041 gggatgctct gggtggggag gcccctcaaa gccttcctgg atggacggtg ccagttcgga
    20101 gtatccctcg gcgcaatatc tcaccggcgt gggacagggg gacaatcggg ccgtggccga
    20161 cgatcaggcc tacgccgccc tggcccgcat cttcaaagcg gaaatctccg cgcactcaca
    20221 agactgggaa tcgtacttgg ttgttgagca gcgtggcacc agtcatgacg agcgacgact
    20281 cacgctcgac caactgacgc gcgtctcaac cgacaaggtg ttggaaaatg ccaaaattct
    20341 cgaccgctgg tacgatgcgg gaaaagggct gtattacgca ttgggaggga tgcatcgtgc
    20401 ccaggtggaa acggccttga tcgagcgcat gaccgagttg gatcgtgccg tatcgtccga
    20461 cgtcgatgag gcgggccaaa cgtcagacag gctggcaaag gtgcgtgcct tgaggcgggc
    20521 gacccgcaat gtggtcctgc gcgaaaccta taatgcggat cttcgtgtcg ttcgggccag
    20581 cggacagggc atgccaccgc gctttcgtct ccaggaatta agtcgcgagt tggaggacta
    20641 cctcgcctca catctcgtac tcaccgtggc catcaccggc gatcatgtgg agccggtgca
    20701 gcgggcattg acagaagggt tgatcaaaga aggactccgc gttgctgccc ttccatcagg
    20761 aacggaaacg gcgagtggag caggacctcg tccaataccg gaattactga ttcgcgggat
    20821 ggtgcgagtc tggcccgtgg acattcgtga cccacagttc acctatgttc gctggtgcag
    20881 cgattttgat atcgttgacc tcgcgggaca gcaaatcatc ggggcgattt cgaaaggcgg
    20941 aaaggaaggg catttatccg agcgggaagc cacggcaaaa gtgatccgag tcatgcagca
    21001 ggacctgtcg gcggatgtcg ccaaggccat cgccgcacat gtgtatggag aatccgaact
    21061 gccggctgag cccattcgcc cagccgggtg tccacgggag gcgttaaaag ccagaccggc
    21121 caatgcaact cattaactcg aaagggaggg agaagaccgg catgagcaga tcgaaaccag
    21181 gagggcgggc gccccgcagc cgcggcaggg gattgacggc aacgcaacaa cgcgttccca
    21241 gccggctggc gaagcggcgg ctgaacgagc gcttaaagag tgacacggca gcttttaatg
    21301 gaggcaatct ggccgataaa ctgcgccgtg aaggatacga agaagtaccc ttggacgaac
    21361 ttcaagaccg gctctcaaaa atgcccggac cattggccgc agtcattctc aaagggaggg
    21421 cctgatccat gccgtattat tatttcgatt cgacggctct cgtgaaacgc tacagcatgg
    21481 aacggggaac gagaatcgtt aataagctga tggtcaagcg aggcaaggtt gcaatcctcc
    21541 ctacctggac cgtcacggag ttgtattcgg ccttttcgaa ccgtgcccag caaggcgaat
    21601 tgacacggga tgattgctac tcggtcatcc ataaattcga acgggaatcg gcccaagggc
    21661 tcttccaatt catcctgcct accatgcaaa cgtatctgtc caccaaagaa ctgatgatgg
    21721 aataccccgg tttacggacg cagcaggtgt tgcatttagc gttggcattg gagttaaagc
    21781 ccctccgact cacggtagtg agcgccgaca cgcagctctt atccgcgagt aaaaccgccg
    21841 gcctgcatat catcaatccc gaggaggatt agccgaatgc cgagtgcgaa tctggctagg
    21901 gacattggta gtcagtaatc attgccgcca cgacaatgac ccaatcatcg gcggtttccc
    21961 gccaacgcgt tgcacgttca accagtgaaa cgggaaggga ttcgctgctg agatctaaca
    22021 gagctcgctg tacgttgccc tgggccaagg cgagacttcc acatacgccg gacgaggctg
    22081 gcaactggcc acccgcccga cggaacaatg ccatagcacg gtagccatgt tcttgtgagc
    22141 ggaacagcga ttccgctcct tggtaaagac ggacggacgc atggttgtcc cggttgagca
    22201 gacccagccg ctttgccatc agcgctgccc tccccatatt catggcggcg ccttcgggat
    22261 catcgctagc gatcgcttcc tccgccttga ctcgcagccg ttccagctcc gcctcgtctc
    22321 ccatcgtctg cgtccatgcc ggatctgtga tacaggcgat tgccaaagtc acgattccga
    22381 tgagagcagg agacagcttc acgaatcctc gcgggctgat aattgaaagc acgaaaggtc
    22441 ttaggccgcc ttattgaagg tactcccagg tgtcaatttg cttgatgctc cagttgacgg
    22501 cctcttttaa cttgaccacc ttgggatcgg catcatggtc gcgaacctga tgcagtgact
    22561 tttgtaacgc ataaagcggt tcatacgctt ttcggtcagg cagtttaccc agagcccacg
    22621 cgacttccgt tttcaacgcg acatcatcgg tcttcaacgt ttcgagcaac ggttcgacga
    22681 tcgtgaaatc cccatggagg gcgcccagga cacccaacgc tttcgctgct gacggtcgca
    22741 gttcggccgg gccgctcgtc atggcctgaa tcaaggtcgg aactgaatcc ttctgaccga
    22801 tatttccgag tgccaacgcg gaagccttgg ctatttctat gtctttgtct ttcaatccgg
    22861 cgaggaggcg gggcacggct ttcgtctcga agagatagcc aaacaagcca atgagccgca
    22921 ctttacgggc ggaagaggtc gaaggatcgt catagagctt gaacaaacgt tcttcctgtc
    22981 cccattcggc cgtgatgagc gctggaaagt cataatcctc cagacgctcc ttcaatttgc
    23041 tcatggcgaa cgccgacggg tcgccggcgt tcgcgactgc agctgcttgt tcggcgtccg
    23101 taaaatccgt tctcacctct ttccgccagg gcatcttttt accttctaac acgtaggaaa
    23161 gttgacaggc aggacctttc cacaagcgcg atggtgaatc cggcaggtcg gcatcgctgc
    23221 ccattttcgt cgtgccttcc caaattttgc gctgctcgca tttgacgttc aacgtcacat
    23281 catgcggttg ggacggatcg gtcaccacgg tatatccgaa ttccttcatc cgcttgacca
    23341 cgacgtccgt taaaggaccg gcatcggtcg atcccttgtc cgagatggca atgacctcaa
    23401 ccagcactcg atcaatcttt tcaagtttct gcttttcttc cgcggtgagc gattcctgac
    23461 gggcccaact gttcgagaga agcagtacga ggagcactag actcgaaagc gccagttgcc
    23521 tcatgtatgc ctccagcatc gatgatgatg agtgatggat aaaaaatgcc ctaaaacgac
    23581 cgataatact gtaccgaaga gccgaaggaa tcagcaagtt ccaagttgaa gcgcttcaaa
    23641 gagcgacagt ataatcgccg gatgttgtct ggcctcacag tcggcttcat cggcgctggg
    23701 aacatggccg aagcgctgat ctccggcctc ctacatggga ggaggatcgc accagatcga
    23761 ctcctcgcgt cagatatcaa cgcttcgaag ttggcccggc ttcgcgaaat c
//
LOCUS       DASTQY010000311         6415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_809568, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000311 DASTQY010000000
VERSION     DASTQY010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809568"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1956)
                     /gene="polX"
                     /locus_tag="VFR82_10265"
     CDS             complement(124..1956)
                     /gene="polX"
                     /locus_tag="VFR82_10265"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEU4502025.1"
                     /translation="MALSTGLAPVEKPREQSSEISSERTKIIHRVDMMMTKRDIAHIL
                     SEIAFYLRLKQDNPYKSLAYERAARALLTCLHEPHQLLAGDALTEIPGIGRGTAAVIR
                     ELLITGASSLHQTIKGSYPSSLVELGDVPGLRPTQIRRLYEEAGIRSVADLKAACRKN
                     QLLDLKGIGPKIQAKIQAALGEFQRGQGYELYANVLAEASKLEKDLKTLEGVETVAIA
                     GALRRKMEVINAFHFVISWPTGRQSAEFITGLKAIPNVSTVVSAESRFVTATSPTGLP
                     IRITLAASADQHFHLLQATGSEEHLEQVLVRFAAQGLDTWDKVRRRVKTLSEKGIYAS
                     AGLPFIPPELREGRGELEVNATKKPPKLIEPSQIEGFFHFHTDYSDGVATVEEMVEAA
                     RHRGYRYIGISDHSQSAFYANGLKEARIRQQWAEIETVQEKYPDIHIFKGIEADILPD
                     GTMDYADALLSEFDFVIASVHSRFNLSEADQTRRVCRALSNPYVTMFGHPTGRLLLSR
                     SGYRLDMRRVLDAAKEYGKVLEINGSRHRLDLDWRDVRLAKAHGIKFSINPDAHAVNE
                     LDNVTLGVNVARKGGLNVEDVINTQSLSAMKTFLRTTRSSETTP"
     gene            complement(2407..3858)
                     /gene="pyk"
                     /locus_tag="VFR82_10270"
     CDS             complement(2407..3858)
                     /gene="pyk"
                     /locus_tag="VFR82_10270"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU4502026.1"
                     /translation="MRKAKIVCTIGPASDHLESLDRLIEQGMNAARLNFSHGTHESHA
                     AAIKTIRQAADQRRAPVCIIQDLQGPRIRVGEVPEGGIRLAERQSVRLQTVLARSGGQ
                     LGHQSLAASGMIELPVTYQHLAQDVRPGARILINDGLIELAVERINGGTIDCKVLIGG
                     TVTSHKGINLPGTRVSAPTLTDKDREDIRFGVAEGVDYVALSFVRGPDDIRAAKKLIA
                     DCGGSTPVIAKIERQEAMMCLGAILEEADGVMIARGDLGVEMGPEAVPLLQKRIIAEA
                     NRRRRLVITATQMLESMTQHLRPTRAEASDVANAVFDGTDALMLSAETAIGAHPVETV
                     QVMDRIIRAAEEGCEPGVLPKRQGDFGNMTLPEAMCTAAASAAAATTASAIVAFSELG
                     STARLISKQRPAAPIIAFTPFEPVRRQMALYWGVQPHTMQQIAQTDERIHEAERRLKA
                     EGLVKPGERIVILSGTRIGQPGGTNLIKLQEVE"
     gene            3966..6275
                     /locus_tag="VFR82_10275"
     CDS             3966..6275
                     /locus_tag="VFR82_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU4502027.1"
                     /translation="MQITTNRLDFVTIEGLLAYAEEFAGQPAAANVHLPTPVQAGDRA
                     KRAHVAMDSLRRFVQDAQRGFSGAAAYRSARRTVIEDVCAGDELVFYAAWNRLLAEGG
                     LQSLLRAPIGSVLKPAHRRPVAIVPRAQLTPQLAEGRIVLDLGDDRFWLLPRDLADRT
                     LLFTMRHGISRVESKTHRVGRRLANQLDHERGVPRADAVGSALARMVGVVGQQLDFLH
                     LSNYLDPKRFLHFISHSPNTRQLYERVVRALSPNSEEHREPTFEHALESSDFGWITGV
                     AKTAEAEALAKAFGVDVSTAKRLTKDPLYCYPGGNSFFDLYVDVIDGLHHLGEAYRGQ
                     VACLYTHSSTLRALMIYLDPRPFHEAFTEFSDYKESQDNVVLLTYEQGRLSGYSTAVG
                     LSERERAARENWVTVERVRKDRVTLKPRQIKRIIALVSGGDFAGAGAALKELRVTGQR
                     HGLKVHFVRHGFLGLANNWIETVTEEDTRGMSSHASSPIGSSRFEDFKEEAVQQAAMR
                     NLKPYLDDAAVVVLGGDGSLRGARAVYEGFGVQVVGMPGTIDNNIEGTVSLGYHSAIA
                     LADQSIESLKATSAAMGSIFFVEVMGAGSGHLALGCAYQSRAEGLLVNEHPDPDAYID
                     DIILGTLKRTLGVPNKSHLFVVAEHTPHRHHREGGVHGLVDYVAGVIAQWPQRQAQSN
                     HYPLTLATKATILGHTLRGAPPVPEDKAIAQHLAYETIQRLVERPEEVVGCMVAYRER
                     GVIDNIPLHAVAPKQFDWDLFARMHGSVS"
BASE COUNT         1317 a         1778 c         1897 g         1423 t
ORIGIN      
        1 ttctacgaaa cacgaactat cagtggcaat ctcaatccag ttgcgatccc tggtgtcgtc
       61 aacggaactt atcagaccac tttccacgtt ggcatgatca acttccgggt gaacttttaa
      121 cattcagggc gttgtttctg aagaccgcgt ggtgcgcagg aaagtcttca ttgctgacag
      181 tgattgagtg ttgatgacat cttcgacgtt cagcccgcct tttcgtgcga cattcactcc
      241 cagtgtcacg ttgtccaatt cgttcactgc atgagcatcg ggattaatgc tgaacttgat
      301 accgtgcgct ttcgcgagac gcacatcgcg ccagtccaaa tccaagcggt gccggctgcc
      361 gttgatttcc aagaccttgc catattcctt ggccgcgtcc agaactcttc tcatatcaag
      421 gcggtaacca gatcgagaga gcagcagacg gcctgtggga tggccgaaca tggtcacata
      481 ggggtttgac aaggcgcgac acactcgtcg ggtctgatcg gcttcagaaa gattaaatcg
      541 actgtgtacg gaagcgatga cgaaatcgaa ttcgctcaac agcgcatcgg cataatccat
      601 ggttccatcg ggcaagatat cggcttcaat gcctttaaaa atatgtatat ccggatactt
      661 ctcttgtacc gtctcgattt cagcccattg ctgacggata cgagcctctt ttaaaccatt
      721 cgcgtaaaac gctgattggc tatgatcaga tatcccgatg tagcgataac ctcgatgccg
      781 agccgcttcc accatttcct ctacggttgc cactccgtct gagtaatccg tatggaaatg
      841 aaagaagccc tcgatctgac ttggttcgat aagcttcggt ggtttcttgg tcgcgttgac
      901 ttcaagctca cctcgacctt cacgtagctc aggagggatg aagggtaggc cagctgacgc
      961 atagatgccc ttttcactca aagttttcac tcgcctccga accttgtccc aggtgtcgag
     1021 gccctgcgct gcgaatcgta cgagaacctg ttcgagatgc tcctcagaac cggtggcctg
     1081 caacagatga aagtgctgat ccgctgaagc agccagtgtg atgcgtatcg gtaatcctgt
     1141 cggagatgtg gccgtcacga accttgattc ggccgatacg acagtactga cattgggaat
     1201 tgctttcagc ccggtgatga actcggccga ttgccgaccc gttggccagc taatcacgaa
     1261 atggaatgcg ttgatgacct ccattttgcg ccggagagcg ccggcgatgg cgactgtttc
     1321 tactccctcg agcgtcttca aatccttctc cagcttggaa gcttctgcta gtacattggc
     1381 gtacagctca tagccctgac cgcgttggaa ttcaccaaga gctgcttgga ttttcgcttg
     1441 aattttcggt ccgatccctt tgagatcaag taattgattt tttcggcagg cggccttgag
     1501 gtccgcgacc gaccggatcc cagcttcctc gtagagcctg cggatttgtg tgggtcgtaa
     1561 tccaggtaca tcgcccagtt ctaccaaaga agacgggtag gaacctttga tggtttgatg
     1621 gagactcgag gcgccagtta ttaacaattc acggatgacc gcggcggtac cgcgaccaat
     1681 gcctggaatc tccgtgagcg cgtcgcctgc taaaagttga tgaggctcat gaaggcaagt
     1741 gagcaaggca cgcgcagctc gttcatacgc cagagattta tacgggttgt cctgtttcag
     1801 gcggagatag aaggcgattt cagacaagat gtgggcgatg tcacgtttgg tcatcatcat
     1861 gtcgaccctg tgaattattt tggttcgttc ggaggagatc tcgctgctct gttctctggg
     1921 cttttcgacc ggtgcaaggc ccgtgctcaa tgccatgctg ctttatctcg aagaccgatt
     1981 gggggcatcc atggatatac gatgatggat tagacagtgt accaacgcca cagttttcga
     2041 ggcgaaggtc tcgctttgaa ggtctggctc tgtggataag attttacaaa gcggcgcgaa
     2101 ggtgaggaca acggaggtat gcctagggag gctaccaggc gggtgtttgc tcacaggcta
     2161 aatccacagg taacaggtgc tgacacaaga tatggtgtaa ttactaccga agacactatg
     2221 cctaaaaaaa aggcagtttg gcattcgtcg tacccttcgc tcgcgccgtc aatcttggat
     2281 gaagttatac acaagcttat ccacaaaacc tgtgagtcca tagttgactc gcgacgatag
     2341 gcgctggcca aactgttcaa gactagcgaa gactggtggc cctttacgaa acagggtacg
     2401 gctggactac tcgacttctt gaagtttgat aaggtttgtt ccgccgggct ggccgattct
     2461 cgttcccgac aggatgacga tgcgctcgcc tggtttgacc aatccttcgg ctttgaggcg
     2521 gcgttctgcc tcgtgaatcc gctcgtccgt ttgcgcgatc tgttgcatgg tgtgaggctg
     2581 cacgccccaa tagagcgcca tttgacggcg aaccggctca aaaggcgtga aagcaatgat
     2641 gggcgcggcc gggcgctgct tggagatcaa acgggccgtg cttcccaact cgctgaatgc
     2701 gacgatggcg ctggccgtag tggccgccgc tgccgaagcg gctgcggtgc acatggcttc
     2761 gggaagcgtc atattaccga aatcgccctg ccgtttcggg agcacaccgg gttcgcaacc
     2821 ttcctcagcg gctcgaataa tgcggtccat gacttggacg gtctcaaccg gatgagcacc
     2881 gattgccgtt tccgcggaca acatcagggc atctgtcccg tcaaagacgg cattggcgac
     2941 gtctgaggcc tctgctctcg tggggcgtaa atgctgtgtc atggattcca gcatttgcgt
     3001 ggccgtgatc acgagccggc ggcggcggtt ggcttccgct atgatgcgtt tttggagaag
     3061 cggcacggct tccggcccca tttccacgcc aagatcgccg cgcgcgatca tcacgccgtc
     3121 ggcctcctcc aagatggcac cgaggcacat cattgcttcc tgccgctcga tttttgcaat
     3181 gacgggtgtg cttcccccgc agtccgcaat gagttttttg gccgcccgga tgtcgtccgg
     3241 ccctctgacg aaagacagcg ccacgtagtc gaccccctct gccacgccaa accggatatc
     3301 ttcgcgatct ttgtcggtga gagtcggcgc gctcacgcga gtgccgggaa ggttgatgcc
     3361 cttgtgcgag gtaacagttc cgccgatgag caccttgcaa tcgatggtgc ccccgttgat
     3421 ccgttcgacg gccaactcga tcaggccgtc attgatgaga atcctagcgc ccggccgcac
     3481 gtcctgcgcc agatgttggt aggtgacggg cagttcaatc atcccggaag cagcgagcga
     3541 ttgatgaccg agctgacctc cggatcgagc cagcacagtc tgcagccgga ccgattgtcg
     3601 ttcggccagc ctgatgcctc cctcaggaac ctcccccacg cggattctgg ggccttgaag
     3661 gtcctggatg atgcagacag gggcccgtcg ttgatccgca gcctgtcgaa tagttttgat
     3721 ggcggcggcg tgagactcgt gcgtcccatg cgagaaattc agccgcgcgg cgttcatgcc
     3781 ctgctcgatc aagcggtcta atgactccaa gtgatcgctc gcaggaccga tcgtgcagac
     3841 gattttggct ttgcgcatgt aaatgcctca gcccttgaca agaattgaat agaggtccaa
     3901 aaaagacgct aaagaactct tcattcgccg gcgaatcaca gctcggcgta tcctaacgag
     3961 ggagcatgca gattaccaca aatcggctcg atttcgtgac aattgaagga cttttggcat
     4021 acgcagagga atttgccgga cagccggccg ccgcaaatgt acatctgccg acgcccgttc
     4081 aggccggcga ccgggcgaag cgggcccacg tggccatgga ctcgctgcgc cgctttgtcc
     4141 aggatgcgca gcggggattt tccggagctg cggcgtatcg ctcagccagg cgtaccgtga
     4201 tcgaggatgt ctgtgcaggc gatgagctgg tgttctatgc agcctggaac cgtttgctcg
     4261 cggaaggcgg gctccagtcg ctcttgcgtg cgccgatcgg atccgtcctg aagccggcgc
     4321 accggcgtcc cgtagcgatc gttccgcgcg cacagcttac accgcagctg gcggagggac
     4381 ggatcgtgct cgatctgggc gatgaccgat tctggctctt gccgcgggat ttggcggatc
     4441 ggacgctgct ctttacgatg cgtcatggca tatcgcgcgt ggagagcaag acccaccgtg
     4501 tcggccgccg gttggccaat cagctcgacc acgaacgagg cgttccacga gccgatgccg
     4561 tcgggtccgc cctggcgcgg atggtaggcg tcgtgggaca gcaactggat tttctgcatc
     4621 tgtcgaacta cctcgatccc aagcggttcc tgcacttcat cagccatagt cccaacacac
     4681 ggcagttgta tgaacgcgtt gtccgtgcgc tcagcccgaa cagtgaggag caccgggagc
     4741 cgactttcga gcatgcgttg gagtcctcgg acttcgggtg gatcacgggc gtcgccaaaa
     4801 ctgcggaggc agaggcgctg gccaaggcat tcggcgtcga tgtgtcgacc gccaagcgcc
     4861 tgacgaaaga cccgctgtat tgctatccgg gaggcaattc gttcttcgat ctgtacgtgg
     4921 acgtcattga cggactgcac cacctgggag aagcctatcg cggtcaggtc gcctgtctct
     4981 atacgcacag ctcgacgttg cgggcgttga tgatttatct ggatcccagg ccgttccatg
     5041 aggcgttcac ggaattcagc gactacaaag aaagccagga caacgtcgtg ctcctgacct
     5101 acgaacaggg ccggttgtcc ggttactcga ccgcagtcgg gttgtccgag cgggaacgcg
     5161 ctgcccgcga aaactgggtg acggttgaac gcgtgcgcaa agatcgggtg acgctgaaac
     5221 cgcgtcagat caaacggatt atcgccctcg tctcgggcgg cgattttgct ggggccggtg
     5281 cggcgctcaa agagttgcgg gtgacggggc aacgccatgg tctgaaagtg cacttcgtcc
     5341 ggcacggctt cctgggactg gcgaacaact ggattgaaac ggtgactgag gaagacaccc
     5401 gggggatgag cagtcatgcg agcagcccga tcgggagcag ccggttcgaa gattttaaag
     5461 aagaggccgt gcagcaggcg gcgatgcgta atctcaagcc gtatttggac gacgcggcgg
     5521 tggtcgtcct gggaggcgac ggaagtctcc gcggtgcccg agccgtgtat gaaggattcg
     5581 gggtccaagt cgtcgggatg cccggcacga tcgacaacaa tatcgaaggg accgtctcgc
     5641 tgggctatca ttcggccatt gccctggcgg accaatccat cgagtcactg aaggcgacga
     5701 gcgcggcgat gggcagcatc ttctttgtcg aagtcatggg cgccggctca ggccatctcg
     5761 ccctaggctg cgcctatcag tcgcgtgcgg aaggcttgtt ggtgaacgag catccggatc
     5821 cggatgccta catcgacgac atcatcctcg ggacgttgaa acggacgttg ggcgtgccga
     5881 acaaaagcca tttgttcgtc gtcgccgagc acacgccgca ccggcatcat cgggaaggcg
     5941 gcgtgcacgg gctcgtggat tacgtggccg gcgtgatcgc gcagtggccg cagcggcagg
     6001 cccagtccaa tcattatccg ctgacgctcg cgaccaaggc gacgattcta gggcacacct
     6061 tgcgaggcgc tcctcccgtc ccggaagata aggcgatcgc ccagcatctg gcctatgaaa
     6121 ccatccagcg gctcgtcgag cgtcccgagg aagtggtggg ctgtatggtg gcctatcggg
     6181 agcgaggggt cattgacaac attccccttc acgctgtggc gccgaaacag ttcgattggg
     6241 acctgtttgc gaggatgcac ggcagcgtgt cgtgaggggt ggcacaacga ggagcttcgc
     6301 ttgtccaacg tcagccatgc tgcgatgttt tctcagattt ccatcttgtc gtggcggcaa
     6361 acaataaccg taagctgagt atgagtgaca ctattggacc ttctcggcgc aacat
//
LOCUS       DASTQY010000312         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_868925, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000312 DASTQY010000000
VERSION     DASTQY010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868925"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /gene="hisF"
                     /locus_tag="VFR82_10280"
     CDS             <1..666
                     /gene="hisF"
                     /locus_tag="VFR82_10280"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEU4502028.1"
                     /translation="VAAVYDREGADELCFLDITASHENRKTIIDVVERTAARVFMPVT
                     VGGGVRTLDDIRHLLNAGADKVSINTAAVERPEFVREAAERFGTQCIVVAIDAKRTVS
                     ASWEVFTHGGRNASGLNAIDWAQRMERYGAGEILLTSMDEDGRQCGYDLDLTASVSAR
                     VSIPVIASGGVGSLEHLYDGFVTGKADAVLAASIFHFRTFTIPQAKAYLQHRGVPVRV
                     TEG"
     gene            766..1419
                     /gene="hisIE"
                     /locus_tag="VFR82_10285"
     CDS             766..1419
                     /gene="hisIE"
                     /locus_tag="VFR82_10285"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEU4502029.1"
                     /translation="MTTRDFKFDVQGLIPAIVQDWLDGTVLMLGYMNEAALTKTLSTK
                     SVHFWSRSRQTMWEKGETSGNKLLVKEVFIDCDRDTILIKAQPLGPTCHTGERACFFS
                     KLDGAAEEHKSSEAFGAILESVLRIIVDRRAHPQPDSYTTKLFEGGQDKILKKVAEEA
                     GEVLLAAKGGKRDEIIYEVADLFFHSLMVLGYHEVSLREVYQELGSRYGKSGLKRQQ"
     gene            1430..1774
                     /locus_tag="VFR82_10290"
     CDS             1430..1774
                     /locus_tag="VFR82_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEU4502030.1"
                     /translation="MNDCLFCQIIQKRIPAKIVHEDEHTLAFDDINPQAPVHTLIIPK
                     QHVQAVGDLRETDSGLLAQLLLACTKVAKHKGLTQSGYRIVTNTGPDAGQTVLHLHLH
                     VLGGRHMTWPPG"
     gene            1904..>2649
                     /gene="dnaG"
                     /locus_tag="VFR82_10295"
     CDS             1904..>2649
                     /gene="dnaG"
                     /locus_tag="VFR82_10295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU4502031.1"
                     /translation="MGRGLISDDVINQIKDRIDIAEIVGQHVSLTRAGQNLKGLCPFH
                     QEKTPSFTVSSSRQIFHCFGCGAGGNVFTFLTRITGASFPETVKDLGRKVGIEIQDVV
                     SASEPQIAQGHRLEQLNQMAAQWFQQNLQNDRSGAEARAYLAGRGIQEATIERFGIGV
                     APVEWDGLIRWLGKQGCTQQDMAAAGLIIARDNGTGYYDRFRARVMITITDLRKRIVG
                     FGGRVLGDGIPKYLNSPDTPLFKKGQTLFAL"
BASE COUNT          590 a          695 c          796 g          568 t
ORIGIN      
        1 gtggcggcgg tctatgatcg agagggtgca gacgagctgt gctttctcga cattacggcg
       61 tcccatgaaa atcggaagac gatcatcgac gtggtggagc ggacggctgc cagggtgttc
      121 atgccggtca cggttggcgg cggggtgcgg acgctcgacg acatccgtca cttgttgaat
      181 gccggggcag acaaggtcag tatcaatacc gctgccgtgg agcgaccgga atttgtccgg
      241 gaggcagccg aacggtttgg cacgcagtgc attgtcgtgg cgattgacgc caagcgaacg
      301 gtatcggcgt cctgggaggt gttcacgcac ggaggacgga atgcgagcgg tttgaatgcc
      361 atcgactggg cgcagagaat ggaacggtat ggggccggcg agattttatt gaccagtatg
      421 gatgaagatg gccggcagtg tggctacgat ctggacctga cggcttcagt gtctgcccgc
      481 gtgtcaatcc cagtgatcgc atccggaggc gtcggctcgc tggaacatct ctatgacggt
      541 ttcgtcaccg gtaaagcgga tgcagtgctc gcggcctcga tttttcattt taggaccttt
      601 acgattccgc aagcgaaggc ctatttgcag catcgcggag tgcctgtgcg tgtgacagag
      661 gggtgaacaa ccgttctcgc ggcccgtgag aggtacccct gacctctcgc atgcagcggc
      721 cgatcagggc gcctggctca aacaggcgaa ggatgtgtgg ctggcatgac cactcgcgat
      781 ttcaaattcg acgtgcaggg actgattccg gctatcgtgc aagactggct ggatggcacc
      841 gtcctcatgc tcggctacat gaacgaagca gcgctaacga aaacgctatc gacgaaatcg
      901 gtgcactttt ggagccgctc acggcagaca atgtgggaaa agggtgaaac gtccggcaac
      961 aagttgcttg tgaaagaggt gttcatcgac tgtgaccggg atacgatcct gatcaaggca
     1021 caaccgcttg gccccacgtg ccacacggga gagcgagcgt gctttttctc gaaactggat
     1081 ggagccgcgg aagagcacaa gtcatccgaa gcgttcgggg caatccttga aagtgtactg
     1141 cggatcattg tagatcgccg tgcccacccc caacccgatt cctataccac caaactgttc
     1201 gagggcggac aggataagat tctcaaaaaa gtggcagagg aagccggcga ggtcctcctc
     1261 gctgcaaaag ggggcaagcg agatgaaatt atctatgaag tcgccgattt gttcttccat
     1321 tccctgatgg tgcttggtta ccacgaggtc tctctgcgag aggtgtatca agagctgggt
     1381 tcacgatacg ggaagtccgg tctcaaacga caacaatgag gcaatcccga tgaacgactg
     1441 cctgttttgt cagatcatcc aaaaacgcat tcccgccaaa atcgttcatg aggacgagca
     1501 cacgttggcc tttgatgaca tcaatccgca ggcgcctgtt cataccctga tcattccgaa
     1561 acaacatgta caggcagtgg gagatttgag ggagaccgat tccggactct tggctcaact
     1621 gttgcttgct tgtaccaagg tcgcgaaaca caagggcctc acgcagtcag gctaccgcat
     1681 cgtgaccaat actggcccag atgcgggcca gacggtgtta cacctccacc tgcacgtgtt
     1741 aggcggacga cacatgactt ggccgccagg gtgattggcg tccctcatct ccctcgtgtt
     1801 tgacaggttt cccgtgtgtc tgttaacctg accggtcaat ttttccgatc acttacgcat
     1861 accagcccca ccgcgtttgg gggagtgagg agctgcgtct cccatgggtc gaggcctgat
     1921 ttctgacgat gtcatcaatc aaatcaagga tcggatcgac atcgccgaga ttgtcgggca
     1981 gcatgtcagc ttgacccgtg ctgggcagaa tttgaagggt ctctgcccct tccaccaaga
     2041 aaaaactcca tcgttcaccg tcagctccag ccgacagatc tttcactgtt ttggctgtgg
     2101 cgcgggaggc aatgtgttca cgtttttgac cagaatcacc ggagcaagtt tcccagaaac
     2161 ggtcaaggat ctcggtcgca aggtgggaat tgagattcag gacgtcgtct ccgcttcaga
     2221 accccagatc gcccaaggcc atcgtctcga acagctcaac cagatggctg cacaatggtt
     2281 tcagcaaaat ttgcagaacg atcggtccgg agctgaggcg cgagcctatc tggcagggcg
     2341 aggcattcag gaggccacga tcgagcgttt cggcatcggt gtcgcgccgg tggaatggga
     2401 tggattaatc aggtggctcg gtaagcaggg gtgtacccag caagacatgg cggcagccgg
     2461 cttgatcatc gcgcgggaca atgggaccgg gtattacgac cgatttcgcg cacgagtgat
     2521 gatcaccatc accgacctcc gtaagcgtat cgtgggattc ggtggccgtg ttcttggcga
     2581 cgggattccc aaatatttga attcgccgga tacgccgctg ttcaagaagg ggcaaacgct
     2641 ttttgccct
//
LOCUS       DASTQY010000313         6027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_665641, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000313 DASTQY010000000
VERSION     DASTQY010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665641"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..1118
                     /locus_tag="VFR82_10300"
     CDS             177..1118
                     /locus_tag="VFR82_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU4502032.1"
                     /translation="MRRAKLIGVMVAAMLMGTSGWVSALDVADVTREWTADGKKIAAE
                     RAKLPAHDEMVKIPAGTFLMGSDKKVDRNAYQPEFPQRRVYLDAYEIDKYEVTTVQFL
                     KFVLATDRKPLIDWQYEGGNFQETMANHPVMHVSWFEAEAYCQWAGKRLPTSAEWEKA
                     ARGEDGRIYPWGNEPAGLSRANFGRTGLSGPVRDRPERLLLYPPIISVDKYENAVSPY
                     GVFQLAGNVAEWTADWYDPHYYKTAPDRNPKGPEKGTQRAFRGGGWIDSTPSVRPAQR
                     NGTDPNTKMNWLGFRCARDAKEAGEASDGKPAQTSLQ"
     gene            complement(1133..2407)
                     /locus_tag="VFR82_10305"
     CDS             complement(1133..2407)
                     /locus_tag="VFR82_10305"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEU4502033.1"
                     /translation="MTGSEQTDQLRNKTKQFAIGILKLCGPLPKTPETLAIQTPLVKA
                     ATTLAANCRALARAWSEDDFHRNRNLASGDADDCVFWLELLAAAGTDQDHPIQALLDE
                     ASDIMALLSICGEPDSSAQARTEPAEPAAEPPTPSPPVVPTDFHGLRVLSFESRMGAE
                     MARLIQRHNGQPLVVPALREIPIPLQDNSAVFRFGVKLILNQLDILILMTGVGTKALL
                     EILQTRYPLAEIIEALKKTIVVTRGPKPLAVLKTFGVEANITVPEPNTWHDVIATLDY
                     YRPVQGLKIAIQEYGVSKPDMIQDLQKRGAEVFSVPVYRWALPEDTAPLHAAIAEVIN
                     GTIAAMLITNAAQVDHVMQLAEQDKKTELFREWCKKIVVASIGPTASEGLKHYDLPVD
                     FEPSHPKMGVLVKELSEQIHALRQVKLPTQPD"
     gene            complement(2454..3269)
                     /locus_tag="VFR82_10310"
     CDS             complement(2454..3269)
                     /locus_tag="VFR82_10310"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU4502034.1"
                     /translation="MSFLLPLVVIGVILFAPYDLMADNARLAEGDLTPMVLIPAGPFP
                     MGVPPGDRDGGRDEYPRHEVYLNAYMIDTYEVTNGRYLEFVKATGHRVPQNPTNPTRN
                     LWEGDRITESIADRPVVNVDWSDADAYCRWTGKRLPTEAEWEKAAKGNHDWRFPWGNI
                     EPTTKHLNYNQRWIGEKTLMPVGSYEAGKSPYGLYDMAGNVWEWVQDWYDPRYYEKSP
                     KNNPKGPETGTKRVLRGSGWQNETPTVRIFTRVDSDPTVRNESTGFRCARDAK"
     gene            3399..3818
                     /locus_tag="VFR82_10315"
     CDS             3399..3818
                     /locus_tag="VFR82_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4502035.1"
                     /translation="MGRAVHKGLRHVALRVTDLARSRAFYEALLDMRVVWEPDADNVY
                     LSSGTDNLALHQISPAELSAYRPPQAQLLDHVGVILEDREAVDRMYNQIETKVQSMGG
                     TIAKPPKQHRDGSYSFYFSDPDGNVIQALYEPTISQS"
     gene            3821..5449
                     /locus_tag="VFR82_10320"
     CDS             3821..5449
                     /locus_tag="VFR82_10320"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4502036.1"
                     /translation="MTTSWDSLPRDHYLSLVPWCWAQLESADPPGPFPFVAGLAPEVV
                     GSLHEAHSLLASSIDTAISDVFAKRAPLDDPLRSRRLEDAYAELVNARPYLSRHIKCG
                     RRSDGHFQWEIPFDPTKSATVTNGGLRIFHAVKRQAIPIGFDQRSLGPIVGRLLGMLD
                     GSHRMDEIKTVVLSSPREAQPVLSRLVESLHQYECLRSSSEASIVRRWFDVIQDRDTV
                     HLGHAALLYRQRDNAFLFDPWLLPWFAESSVPSLWTSLLPKPAAIFLTHDHDDHVDPR
                     TLLHLPKDTPVVVPSRKNRHRLYYDYPALLRELGFTRVVELAHGDAWPFEGGTVLSVP
                     FYGEDPCDLRMPRNCYLISDRGHNVLVHADSGPTNDGRSALKDHVIARLVEQYGPIDL
                     LFASQQQLLEIRSQAAHAALSHPGKWLDVGENGYLTNDYLADLCATAKARLFVSYATG
                     GADWYPDHLSFMFSERNPARTALLTAHWEPPENLKALVAPHGCGYHYARALDVFRETG
                     GRIEVQPAGEALMPLNLYRAVHGEPPFMKQGARP"
     gene            5482..>6027
                     /locus_tag="VFR82_10325"
     CDS             5482..>6027
                     /locus_tag="VFR82_10325"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEU4502037.1"
                     /translation="MRSLTLVTGVAGFIGFHVARRLIDRGDHIIGLDNVNDYYDVRLK
                     EARLAQLTALNGFQFVKLDLANRQGMKELFSEYPIRRVVHLAAQAGVRYSLINPHAYT
                     DSNIEGFMNILEGCRQVKAEHLVYASSSSVYGGNTRMPFSIHDNVDHPISLYAASKKA
                     NELMAHCYAHLYEIPCTGLRFF"
BASE COUNT         1264 a         1683 c         1753 g         1327 t
ORIGIN      
        1 tttcccatga agctggcgaa gaaggacggg agttggatcg tcgagtatca ccatgtcaaa
       61 atgcgctccg tgtccgatta tctgtttaat ctgccggtga attttcaacc acttgaggag
      121 ttcgacgcct ctccaccggt gtcgaaggaa caatgaccaa tcaaggaggg cgtctcgtgc
      181 ggagagcgaa actgataggg gtgatggtcg cggccatgct catgggaacg agcggatggg
      241 tgtcggcctt ggacgttgcg gatgtcacaa gagagtggac tgctgatgga aaaaagattg
      301 cggctgagcg ggcgaagcta ccggctcacg acgagatggt gaaaatcccc gccggcacct
      361 tcctcatggg cagcgacaag aaagtcgatc ggaacgcgta tcaacccgaa ttcccgcagc
      421 gaagagtcta tctggatgcc tacgaaatcg acaaatatga agtcaccacc gtgcaatttt
      481 tgaagtttgt ccttgccacc gataggaagc cgctcatcga ctggcaatac gagggaggaa
      541 actttcaaga gacgatggcc aatcatcccg tcatgcacgt gtcatggttt gaggcggagg
      601 cctattgcca atgggcgggc aagcgactcc cgacgtcggc ggagtgggaa aaagcggcac
      661 gcggtgagga tgggcgtatt tatccctggg gcaatgaacc ggcaggattg agccgtgcga
      721 acttcgggcg taccggattg tcggggccgg tgcgtgatcg gcctgagcgc ctactgttgt
      781 atccgcctat tatttccgtc gacaagtatg aaaatgcggt cagtccctac ggtgtctttc
      841 agctggccgg gaacgtggcc gaatggactg ccgattggta cgaccctcat tattacaaaa
      901 cggctccgga tcggaatccc aaaggtcctg aaaaggggac gcaacgagcc ttccgtggtg
      961 gcggctggat tgatagtacc ccttcggtca ggcctgccca gcgaaacggc acggatccca
     1021 ataccaaaat gaactggctg ggctttcgct gtgcacgtga tgcgaaggag gccggcgaag
     1081 catcggacgg gaagccggcc cagacaagtc ttcagtaagc ggtcgggtgg cgtcagtccg
     1141 gttgagtggg caacttgact tgccggagcg cgtgaatctg ctcgcttagc tctttgacca
     1201 gcacccccat cttgggatgt gacggctcga aatccacggg aaggtcatag tgtttcagac
     1261 cttcgctggc ggtcggtcca atcgaagcca ccactatctt cttgcaccat tccctgaaca
     1321 actccgtctt cttgtcttgc tcggcgagtt gcatgacgtg atcaacttgt gccgcattcg
     1381 tgatcaacat ggcggcaatt gtgccattga tcacttcggc gatggcggca tgtaagggag
     1441 cagtgtcttc cggcaaggcc caccgataca ccggcaccga gaagacctct gcgccgcgct
     1501 tttgtaagtc ttggatcatg tctggcttcg agacgccgta ttcctgaata gcgattttga
     1561 ggccctgcac cggtcgataa taatccaggg tggcgatgac atcgtgccag gtgttgggtt
     1621 ccggaacggt gatattggcc tccactccaa aggtcttgag aactgctagc ggctttggcc
     1681 cccgcgtaac aacgatcgtt ttttttaggg cttcaatgat ttccgccaat ggataacgag
     1741 tctgaaggat ttccaagaga gctttcgtgc ctacgcccgt catgagaatc aggatgtcga
     1801 gttgattgag gatgagcttc acaccgaaac gaaacaccgc agagttgtct tgaagaggga
     1861 taggaatctc gcgaagggcc ggtacgacca gaggttgccc gttgtggcgt tggatcaacc
     1921 gggccatctc cgcgcccatc cggctttcga aggagagcac gcgcaaacca tgaaaatctg
     1981 tggggaccac cggaggtgat ggagttggtg gctcggccgc cggttctgcg ggttccgtac
     2041 gagcctgcgc tgagctgtca ggctcaccgc aaatcgacag gagcgccatg atgtctgacg
     2101 cctcatccag caacgcttga atcgggtggt cctggtccgt gccggcggcg gcgagcagtt
     2161 ccagccaaaa aacacagtca tcggcgtctc cagaggcgag gttgcggttg cgatgaaaat
     2221 cgtcttcgga ccaggcccgc gccagtgcgc ggcagttggc cgcgagtgta gtggcggctt
     2281 tgacgagcgg ggtttgaatg gcgagggttt caggcgtctt cggaaggggg ccacacagtt
     2341 tgaggatgcc gatcgcgaac tgcttggtct tgttccggag ctgatctgtc tgttccgatc
     2401 cggtcatgac acgtttctat cacggaggcc gcggggaagc tcagcgcaaa cgcttatttt
     2461 gcatcgcgag cgcagcggaa accggtcgat tcgttcctga cggtggggtc gctgtccacc
     2521 cgtgtgaaaa tccggaccgt cggagtctcg ttctgccagc ccgatccgcg caatacgcgc
     2581 ttcgtgccgg tctctggccc tttcggattg ttcttggggc ttttctcgta ataccgggga
     2641 tcgtaccaat cctggaccca ctcccagaca ttgccggcca tatcatatag accatacgga
     2701 ctctttccag cctcgtaact gccgaccggc attaaggtct tttcgccgat ccaacgctga
     2761 ttgtaattca aatgtttcgt tgtaggctcg atattgcccc acgggaaacg ccaatcatgg
     2821 ttgcctttgg cagccttttc ccattctgcc tctgtgggca gccgcttgcc cgtccacctg
     2881 cagtaggcgt ccgcatccga ccagtccacg ttgaccaccg gacggtcggc gatcgattcc
     2941 gtgatccggt cgccctccca caggtttctc gtaggattgg tagggttctg cgggacacga
     3001 tggccggtgg ctttcacgaa ttccagatag cgaccgttcg tgacttcata ggtgtcaatc
     3061 atgtatgcat tgaggtacac ctcatgtcgc ggatattcgt cgcgtccccc gtcccggtca
     3121 ccaggcggga cacccatggg gaacgggcct gcagggatca ggaccatcgg agtcaagtct
     3181 ccttctgcga ggcgtgcatt gtccgccatg agatcgtaag gggcaaaaag gatcactcca
     3241 atgacgacta aaggcaacag gaaagacata aggcactcct gactgtgaag aggcatcgta
     3301 acacaatggc caagtgactc gcaggcttca acagccaaat tcaaattgac ggcgatccag
     3361 aagcccacct actatacgac tgcggaggat ctcgtactat gggtcgagcc gtccacaagg
     3421 ggctccggca tgtggcattg cgcgtgacgg atttggcacg ctcccgggcg ttttatgaag
     3481 cgctcctcga catgcgcgtt gtgtgggagc cggacgccga caacgtgtat ctcagctccg
     3541 ggacggataa ccttgcgctg catcaaatat cgcccgcaga gctgagcgcg tatcggcctc
     3601 cgcaggcgca gttgttggat cacgtggggg tcatcctgga ggatcgggag gcggtggacc
     3661 ggatgtacaa tcagattgag acgaaggtcc aatcgatggg agggacgatc gcgaaaccgc
     3721 cgaagcaaca ccgcgacggc agctactcgt tctatttttc ggatcccgac ggaaacgtga
     3781 ttcaagcgct ctacgagccg acaattagtc agtcatgatc atgactacga gttgggacag
     3841 cttgccgcgt gaccactatc tcagtctggt cccctggtgt tgggcgcaac tcgagagtgc
     3901 cgacccgccc ggtccttttc cgtttgtcgc cggccttgcc ccggaagtcg ttggctcgct
     3961 gcacgaagcg catagcttgc tcgcgagctc gatcgatacg gcaatcagcg acgtattcgc
     4021 aaagcgggcg ccgctcgacg acccgcttcg ttccaggcgg ctcgaagacg cgtatgcaga
     4081 attagtgaat gcccgtccgt acctgagccg gcacatcaaa tgcggtcgca ggagtgatgg
     4141 gcacttccag tgggagattc cattcgatcc aaccaagtcg gccaccgtta cgaacggcgg
     4201 tctccgcatt tttcatgcgg tcaagcggca agccattccg atcggcttcg accagagatc
     4261 gctggggccg atcgtcggca ggctgctcgg catgctggac ggttcgcatc gaatggacga
     4321 gatcaagacg gtggtgcttt cctcaccgcg agaggcccag ccggtgctct cgcgcttggt
     4381 cgaatcgttg caccaatatg aatgtttgcg aagctcgtcg gaagcttcga tagtccggcg
     4441 ttggtttgac gtgatccagg accgtgatac ggtccacctg gggcatgcag ccctcttata
     4501 ccgacaacgg gacaacgcgt ttctctttga tccctggctc ctcccgtggt ttgcggaatc
     4561 gtccgtcccg tcgctctgga cctcgctcct cccaaaaccc gcggcaatct ttcttacgca
     4621 cgaccacgac gatcacgttg atccgcgaac attgctgcat cttcctaaag acacgccagt
     4681 ggtcgtacca agccgcaaaa accgtcatcg cctgtactat gattacccgg cgctcctgcg
     4741 cgagttggga tttacgcgag tcgtcgagct agcgcatgga gatgcctggc ccttcgaggg
     4801 cgggactgtc ctctctgtgc cgttctatgg agaagatccc tgcgatctcc gcatgccgcg
     4861 gaactgttac ctcatcagcg atcgaggcca taacgtgctg gtccacgcgg acagcggacc
     4921 gacaaacgat gggcgatccg ctttaaagga tcacgtcatc gcccgcctgg tggaacagta
     4981 tgggcctatc gatctgctgt tcgcttccca acagcagttg cttgaaattc gcagccaagc
     5041 cgctcatgcc gcactctctc atcccggaaa atggctggac gtgggggaaa acggctatct
     5101 gaccaatgac tatctggcgg atctctgcgc cacggcgaag gcaagattgt tcgtctcgta
     5161 cgcaactggc ggtgcggact ggtatcctga tcatctctcc ttcatgttta gtgaacgcaa
     5221 tcccgctcga actgcactgc tgaccgccca ttgggaacct cctgaaaact tgaaagcgct
     5281 ggtggccccc cacggctgtg ggtatcatta cgcacgggcg ttggatgtat tcagggagac
     5341 cggcggacgg atcgaagtgc agccagccgg ggaggccctc atgccgttga atctctatcg
     5401 cgccgttcat ggcgaaccgc cctttatgaa gcaaggcgcg cgcccgtaac ctagacaacc
     5461 atatcgacaa ggaatgactc catgagatct ctcacgctcg tgaccggagt ggctggattc
     5521 atcggttttc atgttgcccg gcgtttgatc gatcgcggcg atcacatcat cggcctcgac
     5581 aatgtgaatg actactatga tgtgcgactg aaagaagctc gtctcgcgca actcacggcg
     5641 ttgaatgggt ttcagtttgt aaaattggac ttggcaaacc gccagggcat gaaggagtta
     5701 ttctctgaat atcccattcg gcgcgtcgtg cacctggccg ctcaggccgg cgttcggtat
     5761 tcgctcatca atccgcacgc gtacactgac agcaacattg aaggattcat gaacattctc
     5821 gaaggctgcc gtcaggtgaa agccgagcac ctggtctatg cctcctccag ttccgtctat
     5881 ggcggcaata cccgtatgcc gttttcaatt catgacaacg ttgaccaccc tatctcgctc
     5941 tatgcggcca gtaagaaagc caacgagtta atggcgcatt gctatgccca cttgtacgaa
     6001 attccgtgca ctggcctgag atttttt
//
LOCUS       DASTQY010000314         6026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1524699, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000314 DASTQY010000000
VERSION     DASTQY010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524699"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VFR82_10330"
     CDS             complement(<1..1236)
                     /locus_tag="VFR82_10330"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEU4502038.1"
                     /translation="MSKSGLMRLNTDMRVNRIADEVWEIPASEKAGMLVPARVYATQT
                     ILQSMDRGVFDQVTNVACLPGIQRYALCMPDGHWGYGFPIGGVAAFDARQGVISPGGV
                     GYDVNCGMRLIRTNLSLMDVQPRLERLMTELFRRVPAGVGAAGAVRLDRNELTRVMLK
                     GARWCVEHGYGWHQDLSRIEEGGCIGGADPSTVSDQAAGRGINQLGTLGSGNHYLEIQ
                     VASNERIFDAGTATALGITGQDQIVVMVHCGSRGFGHQIASDYLKIFEKAMRRYNITV
                     KDQQLACAPFRSPEGQDYFAAMNCAANMAFANRQVITHSVREAFAAVFSTSAEELGME
                     LVYDVAHNIAKVERYAEGDLLVHRKGATRAFGPSSAELAPEFRQTGQPVICGGSMETG
                     SYLLVGTDRAVQETFGSTMH"
     gene            complement(1229..1663)
                     /locus_tag="VFR82_10335"
     CDS             complement(1229..1663)
                     /locus_tag="VFR82_10335"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEU4502039.1"
                     /translation="MSHAFRFLDDIAIADIAFEAEGDSAEEVFVGATRALLEVLANPM
                     TIADSWKRQVLKTEGTLDDLLIEWLSEIVYWKDAAGVVFRDAPLTLRCENSGWTIKAT
                     LIGAPVDGGRQELRNDVKGITKHRYRMERNGSMWKVTVVVDV"
     gene            complement(1776..2921)
                     /locus_tag="VFR82_10340"
     CDS             complement(1776..2921)
                     /locus_tag="VFR82_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU4502040.1"
                     /translation="MSRSPSQSHKATVFIAASELDSNLYHATKFIAPDPFIYLEIKGE
                     RLLIMNDLEMDRAKSQAAVDRVLSYSEIERRARDHGVAAPGSVDIVHVVLREAKIKQV
                     LVPANFPYSHAARLQELGYQVHAKPDPFYEQRVVKTAEEVRHIEAAQRATEEAVAAAH
                     DRLRQALIKEGQLWLDDEPLTSERIKKLINVKLMERDCVAQHTIVAGGEQACDPHNEG
                     SGPLPAHRSIIFDVFPRSASSRYFADMSRTVVRGTPSAELKRLYQTVLDAQEEAIAQI
                     KDGADGMKIHRGICERFEKAGYKTGLVNGRMQGYFHGTGHGVGLDIHEAPRISRTGSL
                     LLEGHVVTVEPGLYYPGLGAVRIEDMVLVTGDGCRNLTNFSKTFELD"
     gene            complement(3082..6009)
                     /locus_tag="VFR82_10345"
     CDS             complement(3082..6009)
                     /locus_tag="VFR82_10345"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEU4502041.1"
                     /translation="MMTRIFRIREATILNGTVVMIDEGRPDGPRTVTLQSVEAALEIF
                     LERGQADLHVSAAHNGPQGRSALSLSGTFKKAEQQTLAVDEGKRLVFPVQFDGLIEAA
                     NLSIRDAADFLGPRPVPESLHGRVNARSAIKIFPGVAGFDAVLSDLTGNMEQFAVTGK
                     ASLSGLLAPQPTFAVTFSSSPVQISELLDKIPAVWIHPQLPSVMQDRQIKGLVEVVSA
                     TVTGSYVEGPQLAVTGEFHVQKGQALIGESRVPAKDLDAHVVIEAGRIRVTKLSGLYG
                     TIHMSDSKGQLSFLEQGPWLELEISGTMAAGDLLQFLSTTVKSELLSRVLASSRDVEG
                     MASPVFRLVGPPNAITFAGGEVKAQHVNLTSSYLPERLTGLQGRFILAEGETQFDQVT
                     GHLGDLLVQVQGGITGGSGSVFRDLAVRISGDAAHMANLVPAKAIPPGMMEGMASTIV
                     VITGASGAPHMRGELALTDSKVSLPGIMEKPTGAPVTVTFEGDVEQSKHMTLTRVEIA
                     APPIRLPIKGKIQLGDKFFIDASLATGTLSLSSVPEWIVKGGFEAGNLELSLDIKGRE
                     RDWRSWKTTGWLALSNGLMVTKGADGPIHDLYVRLLLTRDTAELKRLSFRLSDSDLTM
                     EGTVRNWATKPVMTAKLESNQMDIDLLIPKGQRAPIRDLLEWLAATSKVSATASIARG
                     RYKHLKFGAMSARMTIQDGVLDLDRMSGQSTNGDIAGRIVVQLPRGEPAEAEIALRAT
                     GLLVEDMYRLAGSKGGGITGEARITGTVRGHGRNPHGIYPTLNGRADLLLENGRIFKS
                     EERAIWKIISILNLPAVLQGKVDLEKEGLPYNKITATVMMRNGLFETENLIIDSPIVK
                     ITAAGNYDLPTDQVDMVWAVSPFGSYSQFLKTIPLFGRLFAGERKGFATAMFSVKGAI
                     EDPEVTYLPMKSFATGLTGLAQLAVDVLKNTVMLPIDLVTPDENKAVSKDVIPQEPAP
                     AVP"
BASE COUNT         1223 a         1849 c         1671 g         1283 t
ORIGIN      
        1 gtgcatggtc gacccgaacg tttcctgaac ggcgcggtcc gttccaacca acaggtacga
       61 ccccgtttcc attgaccccc cgcaaatcac cggctgtccc gtctgtcgaa actcgggtgc
      121 cagctcggcg ctcgacggcc cgaaggcacg cgtcgctccc ttgcgatgta cgagcaaatc
      181 gccttccgca tagcgttcaa ctttggcgat attgtgagcc acgtcataga ccaattccat
      241 gcccagttcc tcagcggacg tggaaaagac ggcggcaaac gcctcccgaa cggaatgcgt
      301 aatgacctgc cggttggcaa aggccatgtt ggccgcacaa ttcatcgcgg cgaagtaatc
      361 ctgcccttcc ggcgaacgga agggggcaca ggctagttgc tgatccttga cggtaatatt
      421 gtatcgtcgc atcgcctttt caaatatttt gagatagtcg ctggcgatct gatgaccaaa
      481 gccgcgggaa ccacagtgaa ccatgacgac gatctgatcc tggccggtga tcccgagcgc
      541 cgtggctgtc ccggcatcga aaatccgctc attggatgct acttggattt cgaggtaatg
      601 attgcccgaa cctagagtcc ccagttgatt gattccacgc cccgccgcct gatcggacac
      661 ggtcgacgga tcggctccgc cgatgcaacc gccttcttcg atccgcgaaa gatcctgatg
      721 ccatccatag ccgtgttcga cacaccatcg cgcgcctttc agcatgacac gtgtgagctc
      781 gttccggtcg agcctgactg ctccagctgc gccgacaccg gctggcacgc ggcggaaaag
      841 ctccgtcatc aggcgctcca agcgcggctg cacgtccatc aatgatagat tcgtgcggat
      901 caaccgcatg ccgcaattga cgtcatagcc cacaccgcca ggggaaatga ctccctgtct
      961 ggcatcaaac gccgcgacgc cgccgatcgg aaatccgtat ccccaatggc catccggcat
     1021 gcagagggca tagcgttgga tgccggggag acaagccacg ttggtgacct gatcaaacac
     1081 acctcgatcc atcgattgca aaatggtctg cgtggcgtac actctcgccg gcaccagcat
     1141 gcccgccttt tccgaggcag gaatttccca cacttcgtcg gcgatcctat tcacgcgcat
     1201 gtcggtattc aaccgcatca gaccactctt agacatcgac caccactgtg accttccaca
     1261 tcgaaccgtt gcgctccatc ctgtaccgat gcttcgtgat gcctttcacg tcattgcgga
     1321 gttcttgcct ccccccatcg acaggagcac cgatcagagt ggcctttatc gtccacccgc
     1381 tgttctcgca acggagggta agcggtgcgt cccggaacac gacgccggcc gcatctttcc
     1441 aataaactat ctccgatagc cattcaatca ggagatcgtc tagggttccc tccgttttca
     1501 gcacctggcg cttccaggag tccgcgatgg tcatgggatt cgccagaacc tcgagcaacg
     1561 cccgggtcgc tccgacaaag acctcctcgg cggaatcgcc ttcggcttcg aaggcaatgt
     1621 cggcaatggc aatatcgtca aggaagcgga aggcgtgcga catgggactg ctgagagaaa
     1681 cggcgtgaca ctcagtgtga ggtgaaggta ccggttatca gactaccgct ccctgcccag
     1741 cgcgagaaaa caagcggtgg acagggaacg gccggttaat cgagttcgaa cgtcttcgag
     1801 aaattcgtaa gattcctgca gccgtcgccc gtgacgagta ccatgtcttc gatccgcacg
     1861 gcgcccagcc caggataata gaggcccggc tcaaccgtga cgacgtgccc ttccagcaac
     1921 aatgatcctg tgcgactaat gcgcggcgct tcatgaatat ccagtccgac gccatggccg
     1981 gtgccatgga agtatccttg catgcggccg ttcaccagac ccgtcttgta tccggctttt
     2041 tcgaatcgct cgcagatgcc tcggtgaatt ttcatgccat cggcgccgtc cttgatttga
     2101 gcgatggctt cctcctgcgc atcgagcacc gtctggtaca accgcttgag ttcggcactc
     2161 ggcgttccgc gcacgaccgt gcgcgacata tctgcaaagt accgtgacga agccgatcgc
     2221 gggaagacat caaatatgat gctgcgatgg gccggcaatg gaccgctccc ttcgttgtgg
     2281 ggatcacacg cctgctcacc gccagcgacg atcgtatgtt gcgccacgca atcgcgttcc
     2341 atcagtttca cgttgatgag tttcttgatg cgttcggatg tcaacggttc gtcgtccagc
     2401 cataattgcc cttccttgat cagggcttgc cgtagccggt catgtgcagc agccacggcc
     2461 tcttctgtcg cgcgctgagc cgcttcaatg tgacggactt cttcggcagt cttgacgacg
     2521 cgttgctcgt agaatggatc cggtttggca tgaacctgat agccgagctc ttgcaatcgc
     2581 gcggcatgac tgtacggaaa attggctgga acgagcacct gtttgatttt cgcctcgcgc
     2641 aagaccacat gcacaatgtc cacacttccg ggtgccgcaa caccgtgatc cctcgcgcgg
     2701 cgttcgatct ccgagtagga caatacacga tcaacggcag cttggctctt cgcccgatcc
     2761 atctccagat cgttcataat gagcaagcgc tcacccttga tttccagata gatgaacgga
     2821 tccggcgcaa tgaatttcgt ggcgtgatag aggtttgaat cgagctcact ggctgcgatg
     2881 aaaaccgtgg ctttatggga ctgcgacggt gatcgtgaca tacgactgga ccccttactg
     2941 tactcaccga gcacgtggcc catccacgcg gcggtcctcc tcgcccggtt gtatgaccct
     3001 cgacgctgta ggcgtggcgg gaaacgaagc cggcgggtat cggcgtatcc tggcggaatt
     3061 taacacgggg tcggtagagg gtcaaggaac agccggagcc ggttcctgcg gaatgacgtc
     3121 cttcgagacg gccttatttt catccggcgt gaccaagtcg atcggtagca tgacggtgtt
     3181 cttcaacaca tcgacggcca attgcgccaa gcccgtaagg ccggtagcaa aagatttcat
     3241 cggcaaatac gtcacctccg gatcttcgat ggcgcctttt acggaaaaca tcgccgtggc
     3301 aaagcctttc cgttcgccgg cgaacagcct gccgaacaac ggaatcgtct tgagaaattg
     3361 cgaataggag ccgaaggggc tgacggccca caccatatcg acttgatcgg tcgggagatc
     3421 gtagtttccg gcagcggtga ttttcacgat aggactatcg atgatcaagt tctcggtttc
     3481 gaacaggccg ttgcgcatca tgaccgtcgc cgtgatcttg ttgtagggca acccttcctt
     3541 ctcaaggtcg accttacctt gcaggacggc cggcaggttc aaaatgctaa tgattttcca
     3601 aatcgcacgt tcctctgatt taaagatgcg gccgttctcg agcaagagat cggccctgcc
     3661 gttgagcgtg ggatagatcc cgtgaggatt gcgcccatgg cctcgtaccg ttccggttat
     3721 ccgcgcctct ccggtgatgc caccgccttt ggagcccgcc aatcgataca tgtcttcgac
     3781 cagcaatccg gtggcgcgta gcgcgatttc agcttccgcc ggctccccgc gcgggagttg
     3841 caccacgatg cgcccggcaa tatccccgtt ggtcgactgt ccggacatcc ggtccagatc
     3901 gaggacgccg tcttgaatgg tcattcgtgc cgacatggca ccgaatttca gatgcttgta
     3961 ccgcccgcgg gcaatggaag ccgtcgcact cactttgctg gtggccgcaa gccactcgag
     4021 taggtcacgg ataggggcac gctgaccttt cggaatcagc aggtcgatgt ccatctgatt
     4081 ggattccaac ttggctgtca tcacgggctt cgtcgcccaa ttgcgcaccg ttccctccat
     4141 tgtcaggtca ctgtcagaga gacggaagga gagccgcttg agttcggccg tatcgcgagt
     4201 gaggagcaac ctcacataca aatcatggat cggcccgtcg gcgcccttgg tgaccatcag
     4261 gccgttactc aacgccagcc accccgtcgt tttccaggaa cgccagtcac gctcacggcc
     4321 tttgatatcc agggacaatt ccagatttcc cgcttcgaac cctcctttga caatccattc
     4381 gggcaccgac gacaaggaca gcgtgccggt cgcaagggaa gcatcgatga aaaatttgtc
     4441 tcccagttga atctttcctt tgatcggcaa acggatcggt ggggcggcga tctctactcg
     4501 agtcagggtc atgtgcttcg attgctcaac atcgccttcg aacgtgacgg tgacgggtgc
     4561 accagtcggt ttctccatga tgccgggaag gctgaccttg gagtccgtga gcgcgagttc
     4621 tcctcgcata tgcggcgccc cgcttgcccc tgttatcacc acaatggtgc tcgccatccc
     4681 ttccatcatc cccggaggga tggctttcgc cggcaccagg ttcgccatgt gtgcggcgtc
     4741 gccactgatc cgtacggcca agtcgcggaa gacgctcccc gagccccctg taataccgcc
     4801 ctggacctgc accaacaagt cgcccagatg gccggtcacc tggtcaaatt gcgtttctcc
     4861 ctcggccagg atgaatcgcc cctggaggcc ggtcaaccgt tccggcaagt aactgcttgt
     4921 cagattcaca tgctgcgcct tcacttcgcc tcctgcgaac gtgatggcgt tgggcggccc
     4981 caccagacga aacaccggcg aggccatccc ttccacatca cgggatgacg ccaggacgcg
     5041 tgacagcagt tcggacttga cggtcgtcga aaggaattgc agcaggtctc ccgccgccat
     5101 tgttccgctg atttccagct ccagccaggg accttgctcg agaaaggaga gttgcccttt
     5161 actgtccgac atatggattg tcccgtagag tccgctcagt ttcgtgacgc ggattcttcc
     5221 ggcttcaatc acgacatgcg catccaaatc tttggcgggt acgcggcttt cgccaatgag
     5281 ggcctgtccc ttctggacat gaaactctcc ggtgacggcc agttgcggcc cttcgacata
     5341 cgagcccgtc accgtggcgg acaccacctc aaccagccct ttgatctgac gatcctgcat
     5401 gaccgacggc aattggggat gaatccacac ggcgggaatt ttatcgagga gctcgctgat
     5461 ttgaaccggc gacgacgaaa acgttaccgc gaaggtcggc tgaggagcga gaaggccgga
     5521 caggctggct ttgcccgtca cggcaaattg ctccatgttg cccgtcaggt ccgagagcac
     5581 ggcgtcgaat cctgccacac cggggaagat cttaatcgcg ctgcgcgcat tcacacgacc
     5641 atgcaacgat tcgggaacgg gacgtgggcc gaggaaatcc gcggcgtcgc ggatgctgag
     5701 gttggccgct tcaatcaggc cgtcgaattg caccggaaat acgagacgtt tgccttcgtc
     5761 aacggccagc gtttgctgtt cagccttttt gaaggtgccg gacagagata gagcggagcg
     5821 gccctggggg ccgttgtgcg cggcagaaac atgcaggtcc gcctgcccac gctcgaggaa
     5881 aatctcgagt gcggcctcca ccgactgcaa tgtcacggtg cggggtccat ccggcctgcc
     5941 ctcatcgatc atgacgaccg tgccgttcag aatcgtggct tcccggatgc ggaagattcg
     6001 cgtcatcatt tggagcgcct cgtcgt
//
LOCUS       DASTQY010000315         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1203448, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000315 DASTQY010000000
VERSION     DASTQY010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203448"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFR82_10350"
     CDS             complement(<1..693)
                     /locus_tag="VFR82_10350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU4502042.1"
                     /translation="MTKFPQAVLCVEADTARSEVTVQLAADRMLDVARFLHDAPEASF
                     DHLTDICSVDYPEDQQRFEVVYHLHSLTYRRRIRLKARVSEENPTIASVTGIWKGAEF
                     LEREVYDMMGINFAGHPDLRRILLPEDYGEGFPLRKDFPAEGRGWRSQFEFIPRLDEP
                     PVELTEGEVPELQKEAFRIDSPTGSRRREELLLNMGPQHPSTHGVLRVVLELDGERIV
                     KATPDLGYLHRGV"
     gene            complement(721..1200)
                     /locus_tag="VFR82_10355"
     CDS             complement(721..1200)
                     /locus_tag="VFR82_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEU4502043.1"
                     /translation="MSFLERQFEANIITTNLDAVVNWARKSALWPMTFGLACCAIEMI
                     ASVSSRYDIDRFGAGVFRASPRQSDLMIVAGTVTRKMAPVIRRIYDQMPEPRYVISMG
                     SCATSGNHYNSYAVVQGVDQIVPVDVYVPGCPPRPEALMDGLMKLQEKIQREKVFVR"
     gene            complement(1424..1795)
                     /gene="ndhC"
                     /locus_tag="VFR82_10360"
     CDS             complement(1424..1795)
                     /gene="ndhC"
                     /locus_tag="VFR82_10360"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU4502044.1"
                     /translation="MADEAVPLNYLPILLFIGITIAFGAIQLLAGRLVRPHRPYRAKL
                     NPYESGSPLFSDARVQFPMRYYIIAMLFVIFDIEVIFIIPWAVRFQSLGLLGLVEMLV
                     FIAILLVGLWYAWKKGALEWD"
     gene            complement(1808..>2254)
                     /locus_tag="VFR82_10365"
     CDS             complement(1808..>2254)
                     /locus_tag="VFR82_10365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU4502045.1"
                     /translation="SLEQGVVITIDYGHAAEDLYGPERSRGTLLCYYSQMAGEDPYVR
                     VGEQDITTHVDFTTLATVGEQAGLSVTGFTNQMSFLMGLGIEDMLAQLAPDSVEFRAA
                     IHLLKPEGMGRTFKILVQHKALDRPVLDGLTFRPFFGSALSRHAAA"
BASE COUNT          497 a          667 c          624 g          466 t
ORIGIN      
        1 gacaccacgg tgcaggtagc ccaggtccgg ggtcgctttg acgattcgct ctccatccaa
       61 ttcgagcacg acccgcagga ctccatgcgt gctgggatgc tgagggccca tgttgagcaa
      121 cagctcttct cggcggcggg aacccgtcgg tgaatcgatc ctgaacgctt ccttctggag
      181 ttcgggaacc tctccctcgg tcagttcaac cggcggttcg tccaatcgcg ggatgaactc
      241 gaattggctg cgccagcccc gaccttcggc agggaaatcc tttctcaacg ggaacccttc
      301 cccataatcc tccggcaaga ggatgcgtcg taaatcggga tgacccgcaa agttgatgcc
      361 catcatgtcg tagacttcgc gctcgagaaa ctcggcgcct ttccagatgc cggtcacaga
      421 ggcaatggtc ggattctcct cggacaccct ggccttgagg cgtatgcggc ggcggtaagt
      481 tagcgaatgc aggtgataga cgacctcgaa gcgctgctga tcttccgggt agtccacgga
      541 gcagatatcc gtgaggtggt cgaacgaagc ttccggcgca tcgtgaagaa agcgagccac
      601 atcgagcatc cgatcggcag ctagctgaac ggtcacctcg gaccgcgccg tgtccgcttc
      661 gacgcagagc accgcctggg gaaatttcgt catcaacgtt tcgatcaggg gctgcatgac
      721 ttatctgacg aagacctttt ctcgctggat cttttcctgt agcttcatga ggccgtccat
      781 gagagcctcg ggccgaggcg gacagccggg cacatacacg tcgacgggaa caatctgatc
      841 cacgccctgc acgaccgcat aactgttgta atggtttccg gaagtggcgc acgagcccat
      901 ggagatcaca taccgcggct caggcatttg atcgtaaatg cggcggatga cgggcgccat
      961 cttacgcgtc accgttccgg ccacgatcat caaatcggac tggcgcggag aggcgcggaa
     1021 aactcccgcg ccgaaccggt cgatgtcata gcgcgacgac acgctggcga tcatttcgat
     1081 cgcacaacag gccaggccga acgtcatcgg ccacagtgcc gattttctgg cccagttcac
     1141 gaccgcgtcc aggttagtcg tgatgatatt ggcctcaaac tgtctctcta agaaactcat
     1201 ctcgctctct cgctaatacg taccgttgtt tggcggtccg ccgaccctct ttccacatca
     1261 aacgccggct gtagcctcgc tcgctctcca tcagggctga ttgatctcgc actgcgcgct
     1321 tggacgagcg ccgcgtttct tacaatgcgc tttgatctgg tgtgcgcgtt cagcgagcaa
     1381 gggagatcaa cagccctgcc gcccgctttc ctgggtgcgc gtctcaatcc cattctaacg
     1441 cacccttctt ccatgcgtac cagagtccca ccagcagaat ggcgatgaac accaacattt
     1501 cgacgagtcc caacagacca agcgattgaa agcgcacggc ccacggaatg atgaaaatca
     1561 cttcgatatc gaaaatgacg aagagcatcg caatgatgta ataccgcatg gggaattgca
     1621 cccgggcatc ggaaaacaac gggcttccgc tttcgtaagg attgagtttg gcacgatacg
     1681 gacgatgggg acgaaccagg cgtccggcca agagttggat ggctccaaac gcaatcgtaa
     1741 tgccgatgaa aaggagaatc ggcaggtagt tcaagggaac ggcttcgtcc gccatcacat
     1801 ctcgattcta ggccgctgca tgccggctca acgccgagcc aaaaaacggc cggaaggtca
     1861 atccatcgag taccggtcga tccagtgcct tgtgttgcac aagaatctta aaagttcttc
     1921 ccataccctc gggcttgagc aggtggatag cggcccgaaa ttcaacactg tccggcgcca
     1981 actgggcaag catatcttct atgcccaagc ccattagaaa actcatttgg ttggtgaagc
     2041 cggtgacgga caagccggcc tgctcaccga ccgtagccaa ggtcgtaaaa tcaacgtgag
     2101 tggtgatgtc ttgctctccc acccggacgt agggatcttc gcctgccatt tgtgagtaat
     2161 agcagagtag tgtgcctcgc gatcgctccg gtccatagag gtcttcggcc gcatgtccgt
     2221 aatcgatcgt gatgacgacg ccctgctcga ggct
//
LOCUS       DASTQY010000316         3393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_627945, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000316 DASTQY010000000
VERSION     DASTQY010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627945"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VFR82_10370"
     CDS             <1..942
                     /locus_tag="VFR82_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU4502046.1"
                     /translation="AGSIHVVDIGIPAAFVDEIDSRTLLLTKEMVREALPARPASSHK
                     GTFGHAGILAGSVGKTGAAALAARAALRVGTGLVTVGVPASVNDVLEAKLLEAMTLPL
                     PETKARTLSRAGVDRILAFMQARTAIAIGPGLSTHPETVELVQSLMKHLDRPSVLDAD
                     ALNALASRASLLTACKIPPVLTPHPGEMARLEPDATSQSVNADRLGTAARFARERGVF
                     LVLKGARTVIARPDGLLAICPTGNPGMATAGTGDVLTGMLVGLLAQGIPSWEAACAAT
                     YLHGKAGDLAAEHLGSAGMIAGDLIEHIPYAFRAISE"
     gene            917..1492
                     /gene="tsaE"
                     /locus_tag="VFR82_10375"
     CDS             917..1492
                     /gene="tsaE"
                     /locus_tag="VFR82_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU4502047.1"
                     /translation="MHSEPSASNRKPRSERVWQLALPSPRHTDRLGRAIGSALQGGES
                     LALYGPLGSGKTALVRGIAAGLGATSSAVTSPTFVLLHEYRGRLPLAHVDLYRLSSPK
                     EVESIGLDDYLSGSTVVVIEWADKAPGILPPDRLEMQLRHRTVQSRSIRLMATGPLSA
                     ALLARTRTKRSSRRPRSGRPPSATAKGLSSR"
     gene            1489..1836
                     /locus_tag="VFR82_10380"
     CDS             1489..1836
                     /locus_tag="VFR82_10380"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEU4502048.1"
                     /translation="MIRVILVGTLFLLCLAFFLQNQEQEVTLRYFFGLFEASTPIYKP
                     ILAGFGIGLLVSLILLFPPWVKGRIELRRKTKALQEAEVDLERLRHSLEKLTGRPAPP
                     VPGDLQRREGVDE"
     gene            1829..>3393
                     /gene="mutS"
                     /locus_tag="VFR82_10385"
     CDS             1829..>3393
                     /gene="mutS"
                     /locus_tag="VFR82_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU4502049.1"
                     /translation="MSEEETTPLMRQYREIKRGYPESILFFRVGDFYEMFYEDAQDAS
                     KLLSIALTSRDKSSADPVPLCGVPYHAAQGYIAKLLKAGRTVALCEQVEDPKAAKGLV
                     RREVVRLYTPGTLVDTELLNPAESNFLAAVTRVPRRTSGEATRVGLAALDVTTGEFWL
                     MEWEGDDQQVADELARLQPRELLHSESDTVVTLSISQLQGIRLCARDQAWFDHEDASR
                     LLQSHFGVQSLDGFGCHDTTAGLGAAGAVWRYFRETQPGASPAHVRRLQRRKSGDAMH
                     LDGATIRNLELVRSLGEEPSSGRNTTLFSVLDRTGTAMGSRLLRDWLLRPLTDCHAID
                     ARLDGVDELKERLSERVAIRAALRTVQDIARLSTRVTLDVAGPRELLALKHSLGALPA
                     VRQQLERCRSTLLAERRDQWDDCRDLYDLIEQAIDPDAPLSARDGGIIRSGYHPRVDE
                     LRKARTEGKGWIAALEVKERERTGIDSLKIRYNHVFGYYIEVTKANLSRVPSDFMRKQ
                     TLVNAERFMTAGL"
BASE COUNT          665 a         1012 c         1018 g          698 t
ORIGIN      
        1 gcgggctcga ttcatgtcgt cgatattgga attccggccg cgtttgtgga cgagatcgac
       61 agccggacat tgctcctgac aaaagaaatg gtcagggagg cacttcccgc gcgtcctgcg
      121 tcatcgcaca agggcacatt cggccatgca ggtatcctcg cgggctcagt cgggaaaaca
      181 ggcgcggcag cgctcgccgc acgggcagcg ctccgcgttg gaaccggctt agtcaccgtc
      241 ggcgtgccgg ccagtgtgaa tgacgtcttg gaggcaaaac tcctggaagc catgacgctg
      301 cctcttcccg agacaaaagc tcgcaccctc agtcgggcgg gcgttgatcg gatactcgcc
      361 ttcatgcaag cccggacggc gatcgctatt ggcccgggac tctcgaccca tcctgagacc
      421 gttgagctcg tccagtcctt gatgaaacac cttgaccggc cttcggtgct ggatgccgac
      481 gccttgaatg cgctggcgag tcgtgcgtca ttgctgaccg cgtgcaaaat ccctcctgtt
      541 ctcactccac accctggtga aatggccagg ttggagccgg acgctacttc acagagcgtg
      601 aatgccgacc gcctgggcac cgccgcacgt tttgcccgcg aacgcggagt gtttctggtg
      661 ttgaagggtg cgcgaaccgt gattgcgcgt ccggatggcc ttctggcgat ttgtccaaca
      721 ggcaatcccg gcatggccac cgctggaacg ggagatgtgt tgactggcat gcttgtcggc
      781 cttctggctc aaggaatccc gtcctgggaa gcggcctgcg cggcgaccta tctgcacggc
      841 aaggcaggag acctggcagc tgagcacttg ggatcggcgg gtatgattgc cggcgacttg
      901 atcgaacaca ttccgtatgc attccgagcc atcagcgagt aatcgaaagc ccagatccga
      961 gcgggtatgg caactggcct taccttcacc gcgtcacacc gatcgcctgg gacgcgcgat
     1021 tggttcagcg ctgcagggag gtgaatcact ggcgctctac ggaccattgg gatctgggaa
     1081 aacagcgctt gttcgcggaa ttgctgccgg cctcggcgcc acatccagcg ccgtgaccag
     1141 cccgacattc gtgcttctcc acgagtaccg tggaagattg ccgctcgcac acgttgatct
     1201 gtatcgactc agttctccca aagaggtgga atcaatcggt ctcgatgatt atctctccgg
     1261 atcgacggtt gtcgtgatcg aatgggccga caaggctcct ggaattctcc ccccagaccg
     1321 tcttgaaatg cagttgcgcc atcgaacggt tcagagtaga agtatcagac tcatggctac
     1381 cggaccgctg tcggctgccc tgctggcccg cacgagaacg aagcgttcct ccagacgtcc
     1441 ccgctcggga cggccccctt cggccaccgc aaaaggactg tcctcccgat gattcgcgtg
     1501 atcctcgtcg gaacgctgtt cttgttgtgc ctggcctttt ttcttcaaaa tcaagaacaa
     1561 gaagtcacgc tccgctattt cttcggattg tttgaggcct ccacgccgat ctacaagccc
     1621 attttagcgg ggttcggaat tggccttttg gtatccctca tccttctgtt ccctccttgg
     1681 gtgaaaggcc gcatcgaact gcgtcgaaaa actaaggcgc tacaggaagc cgaggtggac
     1741 ctggaacgac ttcggcactc gctggaaaaa ctcacgggcc gacctgcgcc gcccgtgccc
     1801 ggggatttgc aacgacggga aggcgtcgat gagtgaggaa gaaacgaccc cgctgatgcg
     1861 gcagtaccgc gagatcaaac gcggctatcc tgaaagcatt ctatttttcc gcgtcgggga
     1921 cttttatgaa atgttctacg aggatgctca ggatgcttcg aaactcctgt ccatcgccct
     1981 gacgtcgcga gacaagtcca gcgccgatcc cgttcccctc tgcggcgttc cttaccacgc
     2041 agctcaaggc tacattgcaa aattactgaa ggccgggcgc accgtcgctc tctgcgagca
     2101 ggtggaagac cccaaggcgg caaagggtct ggtgcgtcgg gaggtcgtgc gcctttatac
     2161 acccggcact ctggtggata ccgaattgtt gaatcctgcc gagtcgaatt tcctcgcagc
     2221 cgtcacccgg gtgccacggc gaacctccgg ggaagccacg cgtgtgggct tggccgcatt
     2281 ggatgtgacc acgggggaat tctggctgat ggagtgggaa ggcgacgacc aacaggtcgc
     2341 cgatgagctg gctcgcctgc aaccgagaga attgcttcat tcggaatcgg acacggtcgt
     2401 gacgctgtct atctcgcaac ttcaaggcat tcggctctgc gcccgggacc aggcctggtt
     2461 cgatcatgag gacgcgtcgc gattgctgca aagccatttc ggcgttcagt cgctcgacgg
     2521 attcgggtgc cacgacacta ccgcaggttt gggcgcagcc ggtgctgtct ggcgctactt
     2581 ccgcgagaca cagcccggtg cttctccagc gcatgtccgg cgtctccagc gtcgcaagag
     2641 cggcgatgct atgcatctgg acggcgcgac gattcggaat ctggagttgg ttcgctcgct
     2701 tggagaggag ccgagcagcg gtcgtaacac gacgctattc tccgtgctgg accgaaccgg
     2761 tacggcgatg ggaagccggc tgctgcgcga ctggctcttg agaccgttga ccgactgtca
     2821 tgcgattgac gcaaggctgg atggagttga cgaactcaag gaacggctca gcgagcgtgt
     2881 ggccatccgt gcggcgctac gaaccgtcca ggacattgca cgcttgagca cccgagtgac
     2941 gctcgacgtt gcggggccgc gagaattgct agccttaaaa cactccttgg gcgctcttcc
     3001 ggccgtccgt cagcagcttg agcgctgtcg atccaccctc ctcgccgaac ggcgcgatca
     3061 atgggacgac tgtcgcgatc tttacgacct catcgaacag gctattgatc cggatgctcc
     3121 actctcggca cgggacggcg gcattatccg ttccggatat caccctcgtg tcgacgaact
     3181 gcggaaagca cgtacggaag gcaagggttg gatcgctgca ttggaggtga aggaacgcga
     3241 gcggacgggc atcgactctc tgaagattcg ctataaccac gtgttcggct actacatcga
     3301 ggtcaccaag gccaatcttt ccagggtgcc atccgatttc atgcgcaagc agacattggt
     3361 caatgcagaa cggttcatga cagccggcct caa
//
LOCUS       DASTQY010000317         8022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1587936, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000317 DASTQY010000000
VERSION     DASTQY010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587936"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VFR82_10390"
     CDS             <1..1182
                     /locus_tag="VFR82_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502050.1"
                     /translation="LIEVEDDGAGLDIDKIRAKAVERGLVRSDIAKNLPEAEAIKFIF
                     LPGFSTAEQVGDQAGRGVGMDVVKRVIESMNGHIDVESIRGVGTKFTLHLPLTLLIAT
                     ALMVRSGEERYGIPLPSVREVTMLTAGSLQQMGERSMLHLGDEAVDVQPLQHLMYRNG
                     STRVEVGKPVVIVRTGTGPVGLAVDELLGRQEIVIKPIGALKSLERSCFGGATIDPEG
                     RVVLVLDPARLISGGEQAAAPLEALSETATGTDTETPFQEAMPQSNVEQKILLIDDSL
                     SIRKFVGRMLETAGYEVDTAVDGEEGLRKASAKNYRLILTDLEMPKLNGYEVIQALRS
                     RSQTQQTPIIVMTTRAGDKHRQMALNVGASSYIAKPVEERALIQELERWIGQESPARK
                     V"
     gene            1237..1725
                     /locus_tag="VFR82_10395"
     CDS             1237..1725
                     /locus_tag="VFR82_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEU4502051.1"
                     /translation="MSLRGHHIATTAVVQTARFLVVRFDRILCALPSQGVRGVLTPAE
                     AGSGNQITAVGITYQDIDLASRLGVTLDPANPDMRVVLYSNGQTNGAVRVEAVIGMIE
                     VEREECRPLPSHFQHEERTWISGTTMCKDELVLILTPEWVLGELGEVVAAGTGPRSLS
                     AS"
     gene            1733..2200
                     /locus_tag="VFR82_10400"
     CDS             1733..2200
                     /locus_tag="VFR82_10400"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEU4502052.1"
                     /translation="MLRAAQKHDQAVSGKRSWNVVVFSVGGKKLAARTEDVGGVAPWI
                     EPLPVPSRTPFVGAMLKRDKDVMPVYDLGGRLSRHPEGDTLLCLVARHVDGPMAICID
                     GVIPSLHNVDVAQILPSKRLDLETLGSFSDEGQEIEIVALQRLGRNRQDHVGS"
     gene            2225..3541
                     /locus_tag="VFR82_10405"
     CDS             2225..3541
                     /locus_tag="VFR82_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502053.1"
                     /translation="MPKILVADDSIAVRKVAERLLTEAGLGVTLAANGEEALALLARE
                     VPDLIVSDVIMPDKSGYEVCAFVRGQAALANTPVLLISGIVNDEVTKQAESCHADGVL
                     KKPFQGTSLKDRVLELLGKKQSAPTPAPAKAAAAPAAPTSPANAQVPDEQGPAARQAV
                     EKLKQVEEELQKERRQTSLLTQRISQLEAQTGRIKELEAQSGRLKELEVQAARMKELE
                     LQSGRMRELEGMLVAEREQTAQAKKAAGELTATLQRLKELESVLKHEREQSAQFKKQV
                     ADLQSQAGRVSELEGALQKERALAVQQTQRITEMQKSVARVQELEAALNAERQAATQL
                     VQQMTAMEKAVNRSQELAQQFAREQQRVEELTRRLADHETGGTKAKEIQELLETERER
                     NAVLARRVSETEMAAQQATRRFEEMAKKLGEIAGLASQLGSGSKRA"
     gene            3689..4618
                     /locus_tag="VFR82_10410"
     CDS             3689..4618
                     /locus_tag="VFR82_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502054.1"
                     /translation="MSGDTSDEFQNELVDLFVQEAHEWLQNIHVALDEIQQGPPPERH
                     AALINTLTAGVTNLGGSAATINLPHVEQASFAALPFIEAIKDPTKSFSVQDFLSLCKQ
                     LGQIHTALTNATGVSFDSEAEGETVEAAPGLSPTEFVETLRKLQSKQATTGPLERNLI
                     KTMIDQMEGQVQAGVQHVGVDVIQGYLDRMSESEEAFVQVVDEQMPNLTAQLSSLADP
                     ANMPDNVLEASIREVTQLRAEAQQVNVTPAMTFFSGLHSLLSVLAQHRMSLAASRVQA
                     ITDRVSVVRKLIHQWVERGRTERAAIGQALPAR"
     gene            4717..6765
                     /locus_tag="VFR82_10415"
     CDS             4717..6765
                     /locus_tag="VFR82_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU4502055.1"
                     /translation="MDERRATDLYRRTEDTLTSVASAIQRQAPLDLTDLSALAQEIAE
                     SIARNDQLVVQALAGPSGSTLITNLINVSILATKVGSGLGYYGEELQRLTLAGLVHDI
                     GLFAVPQSVVTKSGKLTPEERTLIEQHPELGHQLVKKLGGEWQWLALVVRQAHERSNG
                     RGYPNRLKGRDIHEFAQIIGAVDVFDALVTPRPYRRRFFPHEAVRELLVAERSAFPRE
                     IIKALVEQLSAYPLGTLVRLTTGEVGSVVGINVQFPLRPIVEVMSDTIPGSGADKREI
                     DLSLSPLVSITETVESPDVGRVSFRSGALAERRSKTLPTVSDQFTSLLENLDALASAI
                     QGVVESRTSSASDAGPPVTRTDSESTGRSTEQDVLDASFEKEVIGLFALEAHEWLAQI
                     HSALKRLSEGSSGTTRSKLSGIMLQGLSNLARSAATVHLTGIEELALSLLPVLHDVGR
                     HEPRAMAAGFASLKSGLDRIAAAVRQAEGRPPTDELPESSGPEVTKTSPLVAPLRVTT
                     GASLLSALRDLQHVRSRSVQPTRDVLDGVIRLAEQEDGEVTVDDIRRILEELDRMDER
                     FVDEVGRRIPVVSRTLIDLQKDGGSDFVTSPQLAPVLEQVEALHDASETVQAGMITMF
                     LQGLRAFLMVAAYRKTPTLAKRLAAVESRIQALVPMAEQWVSIGRVERAAIAEILPVG
                     "
     gene            7071..7592
                     /locus_tag="VFR82_10420"
     CDS             7071..7592
                     /locus_tag="VFR82_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502056.1"
                     /translation="MPKLVKGWHSEDIHAQARHYVRTYILLPSGFLGLICVLGGIGTL
                     GYQLIASSTYTVATFMTSSALLLLGGLCGWIQTRYHRYLFETAPEVFAARMRSAVQRS
                     HRKTKAGPTIPPIVHRGRSLVPVAYVLGGGLLLSASGWAIVQGAMDAVPAILMPWAGF
                     YWSKLFFWRGIVN"
     gene            complement(7609..>8022)
                     /locus_tag="VFR82_10425"
     CDS             complement(7609..>8022)
                     /locus_tag="VFR82_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502057.1"
                     /translation="TIIKQGTKIDNLVQIAHNVTVGEHDIIVAQVGIAGSTTIGHHVM
                     IGGQAGLADHLTIGDQVMIAARAGVNRNIEPNQIVSGAPIMPHETWMKAQAVIPRLPE
                     LRHLVRNLEQRMEAIESRIKSQAAPKGKRKKRGRA"
BASE COUNT         1655 a         2278 c         2500 g         1589 t
ORIGIN      
        1 ctgatcgaag tggaagacga cggagccggt ctggatatcg acaaaatccg ggcgaaagcg
       61 gtcgagcgtg gcctcgtgcg ttccgacatc gcaaaaaatc ttccagaagc cgaggctatc
      121 aagttcatct ttcttcccgg cttctcgact gccgagcagg tgggtgacca ggcgggccgt
      181 ggcgtgggca tggacgttgt taaacgggtc atcgagagca tgaatggcca tatcgacgtc
      241 gaatccatcc gcggtgtcgg caccaaattt actctgcatt taccgctgac gctgcttatc
      301 gcgactgctt tgatggtccg ttccggagag gaacgctatg ggattccctt gccgagtgtc
      361 cgcgaggtga cgatgctcac ggccggttcc ctccaacaga tgggtgagcg gtcgatgctg
      421 catctcggag acgaagccgt tgacgtgcaa cctctgcaac acctgatgta tcggaacgga
      481 tcgacccggg tggaagtcgg aaaaccggtc gtcattgtgc ggaccggcac ggggccggtc
      541 ggattggctg tggacgagtt gctcggccgc caggaaatcg tcattaagcc gatcggcgcg
      601 cttaaatcat tggagcgctc ctgttttggc ggggccacca tcgatcccga ggggcgcgtg
      661 gtacttgtgc tcgatcctgc acggctgatc tccggcgggg agcaagccgc cgcgccgttg
      721 gaagcgctga gtgagacggc gacaggcacc gacacggaga cgccgttcca agaagcgatg
      781 ccccagtcga acgtcgagca aaagatcctc ctgatcgatg attcgttaag catccgcaag
      841 ttcgtggggc gcatgctcga aacagcaggc tatgaggtcg atactgccgt ggacggagag
      901 gaaggacttc gcaaggcgtc cgcgaagaat tatcggctga tcctgaccga tctcgagatg
      961 ccgaaactca acggatatga agtaattcag gcgctgcgca gcagatcgca gacacaacag
     1021 acgccgatta tcgtcatgac gacgcgcgcc ggtgacaagc accgccagat ggcgctgaac
     1081 gtcggcgcga gttcgtatat cgccaagccg gttgaagagc gggcgttgat ccaggagctg
     1141 gaacgctgga tcggacaaga gtcgcccgca cgaaaagtct agcgacctcc ccggccgccg
     1201 ggccgcgatg aatggaggga ggacagtgat acagttatga gtttgcgtgg ccatcatatc
     1261 gcaaccactg ccgtggtgca gacggccaga tttctggtcg tgcgtttcga caggatactg
     1321 tgcgccttgc cgtcgcaagg cgtccggggc gtcctcacgc cggctgaggc cggttcagga
     1381 aaccagatca ccgctgtcgg catcacctat caggatattg atttggcaag ccggctcggc
     1441 gtcacgctcg atcctgccaa tcctgacatg cgcgtcgtcc tgtattccaa cggtcagacc
     1501 aatggggcgg tgcgtgtcga ggccgtcatc ggcatgatcg aagtcgagcg ggaggagtgc
     1561 cggccgttgc cctcgcactt tcaacacgaa gagcgcacct ggatatcagg gacgacaatg
     1621 tgcaaggacg agttagtgtt gatcctgact ccggaatggg tgctgggtga actaggggaa
     1681 gtcgtggcgg caggcaccgg accacggtcc ctctccgcct cataggtgac gcgtgttacg
     1741 agcagcgcaa aaacatgacc aggcggtcag cggcaaacga tcctggaacg tcgtcgtgtt
     1801 ttccgtcggc gggaaaaagc tggcggcaag aaccgaggat gtcggcggtg tcgcgccgtg
     1861 gatcgagccc ttgccggttc ccagccgtac gccgtttgtg ggagccatgc tgaaacgcga
     1921 taaagatgtg atgccagtct atgatcttgg agggcggctc agccggcacc cggaaggcga
     1981 tacgctgctg tgtctggtcg cccgccacgt cgacgggccg atggcgattt gcatcgacgg
     2041 tgtgatccca tcgctgcaca atgtagatgt cgctcagatc cttcccagca agcgcctgga
     2101 tcttgaaacg ctcgggagtt ttagcgatga gggacaggag atcgagatcg tggcgcttca
     2161 aaggctgggc cgcaatcgcc aggaccacgt agggtcgtaa gttgaacagc gaaaggactg
     2221 atacatgccg aagatcctgg tcgcagatga tagcatcgcc gttcgcaagg tcgccgaacg
     2281 gctgctgacg gaggccgggt taggcgtaac attggctgcc aacggcgaag aagccttggc
     2341 gttgctggcg cgagaagtgc ctgatcttat cgtgtccgac gtcatcatgc ccgataaaag
     2401 cgggtatgag gtctgtgcgt tcgtccgggg acaggccgcc ctggccaata caccggtcct
     2461 gctcatctcc ggcatcgtca atgacgaagt gacgaagcag gccgagtcct gtcacgccga
     2521 tggcgtgctc aagaagccgt tccagggcac ctccctcaaa gaccgggtcc tcgaacttct
     2581 cggcaagaag cagagcgcac caacaccggc tccggccaaa gcggcagccg cacccgcagc
     2641 gcccacgtct ccagccaacg cccaagttcc ggacgagcag gggcccgcgg ctcgccaggc
     2701 ggttgagaag ctgaagcagg ttgaagagga acttcaaaag gagcgccggc aaacgtcgct
     2761 gctgacgcag cggatctcgc aactcgaggc tcagacagga cggatcaaag agctggaagc
     2821 gcagtctggc cgtctcaaag agctcgaggt ccaagcggca cgcatgaagg aacttgagct
     2881 gcagtcgggg cgcatgcggg agcttgaagg catgctcgtt gccgagcgtg agcagaccgc
     2941 tcaggcgaag aaagccgccg gggaactgac ggcaaccctg caacgcttga aagagctcga
     3001 atccgtgttg aagcatgagc gcgaacaaag cgcacaattc aagaaacaag tcgccgatct
     3061 acaaagtcag gccggccgag tttcggagct ggaaggggcg ctccagaaag agcgggcgct
     3121 ggccgttcag cagacgcagc gcatcaccga aatgcaaaaa tccgtcgccc gggtccaaga
     3181 gctcgaggcc gcgttgaatg ccgagcgtca agcggcgacg cagcttgtcc agcagatgac
     3241 ggccatggag aaggccgtga atcgctccca ggagctggcc cagcaattcg cgcgggaaca
     3301 gcagcgcgtc gaggaactca cccgccggct ggccgatcac gaaacgggag gcaccaaagc
     3361 gaaggaaatc caagagctcc tggagaccga gcgagagcgc aatgcggtgt tggcacgccg
     3421 ggtctcagag acggaaatgg ccgctcaaca agcgaccaga cgctttgaag aaatggccaa
     3481 gaagttgggc gagatcgcgg gcttggcttc ccagctgggg agtggatcca agcgcgcttg
     3541 aggcgtccgc gctcatcgta cgcaggcggg ccaaggcatg catgtaacaa gccgcccgat
     3601 ccttcctcca acatccgcac gtgacacgca tgaccgcccg atgtttcggc tcctccggct
     3661 ggtcttgcat aggtgaggga gtgcttccat gtcgggcgat acatcagatg aattccaaaa
     3721 tgaactcgtc gatctgttcg tccaggaggc gcatgagtgg ttgcagaaca ttcacgtcgc
     3781 gctcgacgag atacagcagg gaccgccgcc tgagcgtcac gccgccttga tcaatacgct
     3841 gaccgccggc gtgaccaatc tcggcggatc cgctgccacg atcaatcttc ctcatgtcga
     3901 acaggccagt tttgccgccc tgccttttat tgaagccatt aaagatccaa ccaaatcgtt
     3961 ttccgtgcag gattttctct cactttgcaa acagcttggg cagatccaca cggcattgac
     4021 gaacgccacc ggcgtgtcct tcgactcgga ggctgaaggg gaaacggtcg aggcggctcc
     4081 tggattgtcg ccgaccgagt tcgttgagac gttgcggaag cttcagtcga aacaggcgac
     4141 gacgggaccg ttggagcgaa atctcatcaa gaccatgatc gatcagatgg aaggacaggt
     4201 acaggctggt gtgcagcacg tcggcgtcga cgtgattcaa gggtatctcg accggatgtc
     4261 ggagtcggaa gaggcgtttg tccaggttgt ggacgagcag atgccgaact tgacggctca
     4321 gttgtcttca ctagccgacc cagcaaacat gcccgacaac gtgctcgaag cgtccattcg
     4381 agaggtcaca caattgagag ccgaagcgca acaggtcaat gtcactccgg ccatgacgtt
     4441 tttcagtgga ttgcacagcc tcttatcggt tctcgcacaa catcgcatga gcctggcggc
     4501 ctcgcgggtg caggccatta ccgacagggt gagcgtcgtc cggaagctga ttcatcagtg
     4561 ggtcgaacgg ggaagaaccg aacgggcggc gataggccag gcgctgccag cgagatgacc
     4621 tccgtcgtct gcttgtcttg acatgaccca cgccgattgc atgctgaatt gatccgtcat
     4681 gaaccgggtg ggcgggcctc aatccgcccc atagaaatgg acgaacgacg agctacagat
     4741 ctgtaccgcc ggacagagga cacgctgacg tccgttgcct cggcgattca acgccaagct
     4801 ccgcttgatc tcacagactt atctgcgctg gctcaagaga ttgcggagtc gatcgcccgt
     4861 aacgatcaac tggtcgtgca ggcgttggcg gggcctagcg gctccacgtt gatcaccaat
     4921 ctcatcaacg tgagcatctt ggcgacaaag gtggggtccg gcctcggata ctatggggaa
     4981 gaacttcagc gcctcacgtt ggccgggctg gttcacgaca tcgggttgtt tgcggttccg
     5041 caatccgtag ttacgaagtc ggggaagctg actcccgagg aacgcacctt gatcgagcag
     5101 cacccggaat tgggccatca gttggtcaaa aagctgggcg gggaatggca gtggctcgcc
     5161 ctggtcgttc ggcaggccca tgaacgctcc aatgggcgag gctatccaaa ccggctaaaa
     5221 ggtcgcgaca tccatgagtt tgctcagatc atcggcgccg tcgatgtgtt cgatgccctc
     5281 gtcacgccgc gtccgtatcg tcgccggttt tttccgcatg aggccgtccg cgagttgctg
     5341 gtcgcagagc ggtcggcctt tccgcgtgaa atcatcaaag cgctggttga acagctttcg
     5401 gcctatccgc tcggcacgct ggtgcggctc acgaccggcg aagtggggtc ggtcgtggga
     5461 atcaacgtgc agtttccact gcgtccaatc gtagaagtta tgtccgatac cattcccgga
     5521 agcggagcgg acaaacgcga gatcgatctc agcctgtcgc cgctcgtgtc cattaccgaa
     5581 acggtcgagt ccccggacgt tggacgggtg tcgtttcgct ccggcgctct tgctgaacga
     5641 cggtctaaaa cgcttccaac ggtatccgat caatttacgt ccttgctcga aaatctggat
     5701 gcgctggcca gcgccattca gggcgtagtc gagtctcgaa cgtcgtcggc atccgacgcg
     5761 gggccgcccg tgacgcgaac agacagtgag tctaccggtc gttcaaccga gcaagatgtc
     5821 ctggatgcca gctttgagaa agaagtgatc ggtctcttcg cgctcgaggc gcatgagtgg
     5881 ctggctcaga tacactcggc cttgaagcgg ctcagcgaag ggtcgagcgg aacgaccaga
     5941 tccaagcttt ccggcattat gctgcaggga ttgagcaatc tggcccgttc tgcagccacc
     6001 gtgcatctga ccggcatcga ggagctggcc ttgagtctcc ttccggtcct tcatgatgtg
     6061 ggacgccacg aacccagggc catggctgcc gggttcgcct cgctgaagtc gggccttgat
     6121 cggatcgccg cggcggttcg tcaggcagaa ggaaggccgc cgaccgatga gcttcccgaa
     6181 tccagcggac cggaagtgac caagacttcc cctcttgtcg ccccgctgcg agtgactacc
     6241 ggcgcatcat tattgagcgc attgcgcgac cttcagcatg ttcgatcccg atccgtgcaa
     6301 ccgactcggg atgtgctgga tggggtcatc cgcctggcgg aacaggaaga cggggaggtg
     6361 accgtcgacg atatccggcg cattttggaa gagctggatc ggatggatga acgatttgtc
     6421 gacgaagtcg gccgacggat tcctgtcgtg agccgcactc tgatagacct gcagaaagat
     6481 ggcggaagcg attttgtcac gtccccccag ctcgcgcccg tcctcgagca ggttgaagct
     6541 ctccatgatg cgtcagaaac cgtacaagcg ggcatgatca cgatgttcct acaaggcctt
     6601 cgagcattcc tgatggtggc tgcgtaccgg aaaactccga cgctggcgaa acggcttgca
     6661 gcggtcgagt ccagaatcca ggcgttggtg ccgatggctg agcagtgggt gagtatcggg
     6721 cgcgtcgaaa gagcggcgat tgcagagatc ttgccagtcg gctagtcacc gcgttccatc
     6781 ccgttcagcg gtttgttacg ttttgtcttt caccagctgc tacccggatc gatcctcttc
     6841 gccaatccag actctcttcg taccccgtca aggtgttttt gattgcactg ccgtgagatg
     6901 gcttgcgcag gtgattggcg atctgctatc cgaggcgtca tctggagtgc ggtcgccata
     6961 tccacatccg gtgtagatat cttgcagaca acgtgagcat cggcgatgtc tgcttcactc
     7021 tccgaagaat gctatgatgc aactccaagc ggtttctacg gcgagtcacg atgcccaaat
     7081 tggtgaaggg ctggcacagc gaggacattc atgcccaagc ccggcattac gtccggacgt
     7141 atattctgct cccttccgga ttcctgggac tgatttgcgt gctgggggga atcgggacgc
     7201 ttggttatca gctcattgcg tcgagcacct atacggtggc gacgttcatg acgagttccg
     7261 cgcttctgtt gcttggcgga ctctgcgggt ggatacagac gcgctaccat cgatatctct
     7321 tcgaaacggc tcccgaggtg ttcgccgctc ggatgcggag cgccgttcag cgatcacacc
     7381 gaaagaccaa agccggaccc acgattcctc ccatcgtcca tcgggggcgc agtcttgtcc
     7441 cagtggcata tgtgctggga ggcgggctgc tgttgtccgc gtcaggctgg gccatcgtgc
     7501 agggggcgat ggatgcggtg ccggcaatcc tgatgccctg ggccggattc tattggagca
     7561 aactgttttt ctggcgggga atcgtcaact aagcgggtga gatccttctc atgcccggcc
     7621 gcgctttttc cgtttacctt tcggcgctgc ctggctcttg atgcgggatt cgatcgcttc
     7681 catccgttgt tccaggtttc tgaccaagtg ccgcagctca gggagccggg gaatgacggc
     7741 ctgggctttc atccacgtct catgcggcat gatgggcgcc cctgacacga tctgattcgg
     7801 ctcgatattg cggttgacgc ctgcccgggc ggcaatcatc acctggtctc cgattgtcag
     7861 atgatccgct agtccggcct gccctccgat catgacgtgg tgaccgatcg tcgtactgcc
     7921 tgcgatcccc acttgagcca cgatgatgtc gtgctcgccc acggtgacgt tgtgtgcaat
     7981 ctgcacgaga ttgtcgatct ttgtgccctg tttgatgatc gt
//
LOCUS       DASTQY010000318         5994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_15951, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000318 DASTQY010000000
VERSION     DASTQY010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15951"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VFR82_10430"
     CDS             complement(<1..876)
                     /locus_tag="VFR82_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4502058.1"
                     /translation="MKKPGTAEPGGEDMASKYVALPKGVSEQTFAAAVKEFRRVLGED
                     NVLTDADHLAPYTKVMMPVPEANHTPSAAILATTVEQIQKIVGICNKYKVPIWTISTG
                     KNLGYGSAAPAERGQIVLDLHRMNRILEVDGDLCYALVEPGVTYRQLYDYFQEHKLPL
                     WLSVPAPSAIAGPTGNALDRGVGYTPYGEHFMNACGMEVVLANGEILRTAMGGLPKSN
                     TWQVFKWGYGPYLDGIFTQSNYGIVTKFGFWLQPAPPVYKPFMIQYQNEEDIVEIVEI
                     MRPMRLNGVIPKSGVI"
     gene            complement(884..1762)
                     /locus_tag="VFR82_10435"
     CDS             complement(884..1762)
                     /locus_tag="VFR82_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502059.1"
                     /translation="MDVDRRTLMKGMLASGALLALGVPSWALGDQSARRSKHCLLVLS
                     GTGADEAFARGTQAACAAMTYDGLRTVQLKGGLLAGMDQMVNLLGHSRGARMIAILDD
                     ASAVIFLELARSSGVRMLSMGTHACSTDGACRIRHELASTSQGHSAGGLLASQLRHQQ
                     SSFSIEESFLHDASPGLALTAWSAPGFCSYRSGESESIHLHCSGLSLPDGCGLLALDT
                     TEQWIPISPQAYDRDSTASPSENWVESVGYAVTASALGMDSVKESCSGRAFVHQATES
                     ECSGPQERFMSFVMDL"
     gene            complement(1774..2106)
                     /locus_tag="VFR82_10440"
     CDS             complement(1774..2106)
                     /locus_tag="VFR82_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU4502060.1"
                     /translation="MKVGRMELVMPMSGLIVMASLTAAMGAGQNDVSQPGFAWKDGAE
                     VYTKICALCHDASVGPPIRGRSLDPTYIQLIVRHGSRAMPSFRASEIDDQSLEQLAEY
                     VSKSAADK"
     gene            complement(2316..2663)
                     /locus_tag="VFR82_10445"
     CDS             complement(2316..2663)
                     /locus_tag="VFR82_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU4502061.1"
                     /translation="MRRQRLPSLLMLGLVMVAPMFIHAADVDAQRGRTIYEKNCLLCH
                     GPEGKGDGQFGKTISPPAADFTSAASKKKTEAQLLATIENGRPPTAMEAWKGQLSGRE
                     IQDVLAYVLTLRK"
     gene            complement(2705..3271)
                     /locus_tag="VFR82_10450"
     CDS             complement(2705..3271)
                     /locus_tag="VFR82_10450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU4502062.1"
                     /translation="MADEVKMLGFAGSLRRQSYNRAALGAAVKLVPSGITLNTFDLDG
                     IPPFNQDHEKKPPDAVRQFKAAILKADAILMVTPEYNYSVSGVLKNAIDWASRPYGES
                     AWDGKPVGVMGASVGMLGTCRAQYHLRQMFVFLNMLPLNQPEVMIANADEKFDEEGNL
                     KDEKTAQKIRELLEALADWTRRLKTAKA"
     gene            complement(3324..3686)
                     /locus_tag="VFR82_10455"
     CDS             complement(3324..3686)
                     /locus_tag="VFR82_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU4502063.1"
                     /translation="MMRHIFRAIVLGCVLVASGWCVTAQWVSAAAGDAAKGKTIFQSK
                     CVTCHGPEGKGDGPLGKKLKPPAGDFTSAESKKKPEAELLEVIQNGKPKTSMVAWSKQ
                     LNEAEIQDVLAYVLTLRK"
     gene            complement(3717..3911)
                     /locus_tag="VFR82_10460"
     CDS             complement(3717..3911)
                     /locus_tag="VFR82_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502064.1"
                     /translation="MVNDPGPVHSTTPAPPREIPGETAIYRHEPSGIMERSGSIPVWL
                     KVVAFGLIVWGVYYAWRYAY"
     gene            complement(3919..>5994)
                     /locus_tag="VFR82_10465"
     CDS             complement(3919..>5994)
                     /locus_tag="VFR82_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HEU4502065.1"
                     /translation="VAVVHSLWMIIPVITVIVNFFGTMLGRWGAILGGGGGDNYAAKF
                     LMIGVVYYLIGSVQGSIETLHRFQQLTHFNDFVVGHAHLTVFGAMIMWVVGGLYYVWP
                     RLTGRQLWSDRLASGHLWLTITGFTAMVLGLTAQGFIQGAMLDNGADFVDSIKEMQPW
                     WVARTIVGTIMDVALGFMAYNFYKTVREGKPVESEDRDPLGRAGSPIPMTTTKNWLEN
                     PSTVGIVAGVIFFVGSFFILGGLPWLQAATRTTMVADAVTGLSVQVKQYSPLEERGRQ
                     VYIHDGCWQCHSQYVRPVTGESLRWGPVSQVGESAFDRPHMFSTRRIGPDLTRVGRKY
                     GDDWHIAHLWKPQEVVPDSIMPRFTWLFEEPKGDAPPQLTEDGKALVAYLQRLGTAIG
                     DWRAGSVSWQQTSGAAMLRGSPEGREAVLSLGQAVYERRCAGCHGAKGDGNGPSGQFL
                     DPKPRDFTKGLFKFRSTKGKDSLPTDADLYVTITHGLWGTSMPSMHELPDRQRWAVIQ
                     FLKSFSDRWMKEPAGQPIAIPPEPPVTPISITKGSALFQSNCALCHGEQGKADGALSE
                     PGMLVDDWEHPIRPAHLSLPAGAPGGVKLGHESARLFQTIMAGIGGTPMPPFDSLTAE
                     EVWDMVHFVQSLRVTAHEAELVAVGLQEQDRKTARERIWAAMSGGAAGERTVAADAGR
                     AIASDMAQH"
BASE COUNT         1251 a         1839 c         1611 g         1293 t
ORIGIN      
        1 gatcacccct gatttgggaa ttacgccatt gagacgcatc ggacgcatga tctccacgat
       61 ctccacgatg tcctcttcat tctgatattg aatcatgaac ggtttgtaca ccggcggcgc
      121 tggctgaagc caaaaaccaa atttggtcac gatcccgtag ttggactgcg taaagatccc
      181 gtcgagatag ggcccgtagc cccacttgaa aacctgccag gtattggatt ttggcaagcc
      241 acccatggca gtgcgcagaa tctcgccgtt tgccaacacc acttccatgc cgcaggcatt
      301 catgaaatgt tcgccgtacg gcgtatagcc gacaccgcgg tccaacgcat tgccggttgg
      361 tccggcaatg gcggagggcg ccggcacgga gagccataag gggagtttgt gctcttggaa
      421 ataatcgtag agttgccgat aggtcacgcc cggttcgacc aaggcgtagc agaggtcgcc
      481 gtcgacctcg agtatgcgat tcatgcgatg cagatcgagg acgatctggc cacgctccgc
      541 cggagccgct gatccgtagc ccaggttctt gcccgtggag atcgtccaga tcgggacctt
      601 gtacttgttg cagatcccca cgatcttctg gatctgctcg acggtcgttg ccaagattgc
      661 ggcagagggg gtgtgattcg cctccggcac cggcatcatg acctttgtat agggtgcaag
      721 gtgatcggcg tcggtaagca cgttgtcctc acccaatacc cggcgaaact ctttcactgc
      781 ggcagcaaac gtctgttcag agactccctt cggcagtgcg acatatttac tggccatgtc
      841 ttctccccct ggttcggccg tcccgggctt tttcaacttc ctgctaaaga tccatcacaa
      901 atgacatgaa tcgttcctgt gggccgctgc actcgctctc agttgcttga tgcacgaacg
      961 cgcggccgga gcagctctcc ttgaccgaat ccataccaag cgccgacgca gtcacggcat
     1021 aaccgacaga ttccacccag ttctcggacg gcgatgcggt ggagtcgcgg tcgtacgcct
     1081 gcggcgagat gggaatccat tgttctgtgg tatcgagagc gagcagccca cacccgtccg
     1141 gaagggagag gcctgaacaa tgcaggtgaa tcgactccga ttcccccgat cgataggagc
     1201 agaatcccgg cgcagaccag gcggtgagag caagtcctgg cgatgcgtcg tggagaaaac
     1261 tttcttcgat cgaaaaactg ctctgttggt gtctcaattg tgatgccagg agaccacccg
     1321 cactgtgacc ttgcgaggtc gaggcgagct cgtgtcggat acgacaggcc ccatcggtgg
     1381 aacaagcatg cgtacccatg gatagcatcc gaactccaga cgacctcgcc agttccagaa
     1441 agatcactgc gctggcatcg tcgagaatgg cgatcatccg ggcccctcga gagtgaccca
     1501 gcagattgac catctggtcc atgccggcta acaacccacc tttcagttgc acagtccgca
     1561 acccgtcgta ggtcatggcg gcgcaggccg cctgcgtgcc gcgggcgaat gcctcatccg
     1621 caccggtgcc gctcaatacc aacagacagt gtttacttct tcgcgcagac tggtccccga
     1681 gcgcccatga cggaaccccc agagccagaa gcgccccact ggccaacatc cccttcatca
     1741 acgtacgccg atcaacgtcc atggtccgcc tccctacttg tcggctgctg atttcgacac
     1801 atactcggcg agttgttcca atgattggtc atcgatttcg gatgcgcgga aggaaggcat
     1861 ggcacgactt ccatgtcgca cgatcaattg gatgtacgtg ggatcgaggg agcgtccccg
     1921 aatgggtggc ccgaccgacg cgtcatgaca cagagcgcag atcttcgtat agacctcagc
     1981 cccgtctttc caggcgaatc ccggctgaga tacgtcgttc tgaccggctc ccatcgcggc
     2041 cgtgaggctt gccatcacga tgagtcctga cataggcatt accaattcca tccgtcctac
     2101 cttcatgagg acctcctcgt ttgacctttc atctttaaac cgccgattaa taggcccgcg
     2161 atcgcgataa cgacggaaag gcaattgcgg ccgcggcaca ccggcacagt gaaaaagctt
     2221 ttgcgtaccg cctccctcca aaaaaacaaa aatccgtaac catgtgcatc gcggtatagg
     2281 gcccgcactt tgccgctctg taaatcgatg tccactcact tccgcaacgt caacacataa
     2341 gccaagacat cctgaatctc acggccggat agttgtccct tccaggcttc catcgccgtc
     2401 ggcggtcggc cattctcgat cgtcgccagc aattgagcct cggtcttttt cttgctcgcc
     2461 gcgcttgtga aatcggcggc cggcggagat atggtcttgc cgaattggcc atcgcctttt
     2521 ccttcaggac cgtggcaaag gagacaattt ttttcgtaga tcgttcgccc ccgttgagca
     2581 tcgacatcgg ccgcgtgtat gaacatcggt gcgaccatga cgagacccag catcaacagg
     2641 ctcggcaggc gctgacgcct catcgatgtc tcttgacgat ccacccgcca tctttcctca
     2701 tcgctcaagc cttcgcagtt ttgagtcttc tcgtccagtc ggcaagggcc tcaagcagtt
     2761 ctcggatctt ttgagcagtc ttttcgtcct tcaaattacc ttcctcatcg aacttttcgt
     2821 cggcattggc gatcatcact tccggctggt tcagcggcaa catgttcaag aagacgaaca
     2881 tctgccgtag gtgatactgc gcgcggcatg tcccgagcat accgacagag gcgcccatga
     2941 cacccactgg cttcccgtcc caggcgcttt caccataggg acgggaggcc cagtcgatgg
     3001 cattcttgag cacgcccgaa actgaatagt tgtactccgg cgtcaccatc agaatcgcat
     3061 cagccttcaa tatcgccgcc ttaaattgcc gcaccgcgtc aggcggcttt ttctcatggt
     3121 cctggttgaa ggggggaatg ccgtcgagat cgaacgtgtt gagcgtgata ccggaaggaa
     3181 cgagtttgac tgcagccccg agtgcggctc ggttatagga ctgtcttctg agacttcctg
     3241 caaaacccag catttttact tcatcagcca tggccagttc tccccatcat gacggcaaac
     3301 gtcccatgct catcgtgttc tgactatttt ctcagtgtca gcacataggc cagcacgtcc
     3361 tggatttcag cttcgttgag ctgtttgctc cacgccacca tggaggtctt tggtttgccg
     3421 ttttgaatga cctcgagtag ctccgcctcg ggcttcttct tagattcagc gcttgtaaaa
     3481 tcgccggctg ggggcttcaa ctttttgcca agcggtccgt cgcctttacc ttccggtccg
     3541 tgacaggtca cgcatttgga ctgaaatatc gttttgcctt tggcagcatc gcctgcggca
     3601 gcggaaaccc actgcgctgt cacacaccag cctgatgcca cgagcacaca accgagcacg
     3661 atcgcccgaa agatatgacg catcatgtcc tccttcttag gatgttgttt catcgctcaa
     3721 tacgcatagc gccacgcgta atacacgccc catacaatca agccgaacgc gacaaccttg
     3781 agccagactg gaatggagcc gcttcgctcc ataattccag acggctcatg ccgatagatg
     3841 gcagtttcac ccggtatttc gcgtggcggt gcgggcgtcg tggaatggac tggaccggga
     3901 tcgttcacca tgacggcctc agtgctgtgc catgtcggat gctatggcgc gccctgcatc
     3961 ggctgcgacc gttctctcgc cggccgcgcc tccggacatc gccgcccaaa ttctctctcg
     4021 tgcggtcttc cggtcttgct cctgaaggcc gaccgcaaca agttcggctt cgtgagccgt
     4081 cactcgaaga gattgaacaa aatgcaccat gtcccagacc tcttccgcag tcagactgtc
     4141 gaatgggggc atcggtgttc cgccgatgcc ggccatgatg gtctggaata gacgcgcgct
     4201 ctcgtgcccg agtttcacgc cgcctggcgc accggccggc agtgacaggt gcgccggcct
     4261 aatgggatgc tcccaatcgt cgaccagcat gcctggttcg ctcagcgcac catcagcttt
     4321 gccctgttct ccgtggcaga gcgcgcagtt gctttgaaac agcgcgctgc ctttggtaat
     4381 ggatatgggt gtaaccggcg gctctggagg gatggctata ggctggcccg ctggctcctt
     4441 catccatcga tcggagaacg acttcaggaa ctggatcacc gcccaccgct gccggtcagg
     4501 aagttcgtgc atagagggca tcgatgtgcc ccaaagacca tgtgtgatgg tgacataaag
     4561 atcggcatcg gtcggtaagc tgtcctttcc cttggtggat cggaatttga acaggccttt
     4621 tgtgaagtct cgaggcttgg gatcaagaaa ttgccccgag ggaccgttac catctccctt
     4681 ggcgccgtga cagccggcgc aacgtcgttc gtagactgct tgaccgagag acagcactgc
     4741 ctctctgcct tcaggactcc cacgcagcat ggctgctccg gaggtctgct gccaggacac
     4801 tgagccggct cgccaatcgc caattgccgt cccgagccgc tgcaaatagg ccacaagagc
     4861 ctttccatct tcagtcagct gcggaggcgc atcccccttc ggctcttcaa agagccaggt
     4921 gaaccgcggc atgatggaat ccggcacgac ctcctgcggt ttccagagat gcgcaatatg
     4981 ccagtcgtcg ccatacttcc ttcccacacg agtcagatcc gggccgatgc ggcgcgtact
     5041 gaacatatgc ggccgatcaa aagctgactc acccacttga gatacaggtc cccatcggag
     5101 cgattctccc gttaccggac ggacgtattg cgaatgacat tgccaacagc cgtcatggat
     5161 gtacacctgc cgtccgcgct cctcgagggg agaatactgt ttcacctgga cggacaggcc
     5221 ggtgacggca tcggcgacca tggtcgtgcg tgttgcggcc tgaagccagg gaagaccgcc
     5281 aaggatgaaa aaggagccga caaagaaaat gacacctgcc acgatgccga ccgtcgaggg
     5341 attttccagc caattcttgg tcgtcgtcat ggggatcggt gagcccgccc tccccaaagg
     5401 gtcacgatct tcgctttcca ccggcttacc ctcgcgcacc gtcttataga agttgtacgc
     5461 catgaaccca agcgcgacat ccatgatggt gcccacaatc gttcgggcta cccaccacgg
     5521 ttgcatttct ttgatcgaat ccacgaagtc ggcgccgttg tccaacatcg ccccttgaat
     5581 aaagccctgc gccgtgagcc cgaggaccat cgccgtgaac ccggtgatgg tgagccacag
     5641 atgcccgctc gcaagtcggt cgctccagag ttgtctgccg gtcagccggg gccagacgta
     5701 atacagccct ccaaccaccc acatgatcat ggcgccaaac accgtcagat gggcatggcc
     5761 aaccacgaaa tcgttaaaat gagtcaattg ctgaaagcgg tgtagagttt cgatggagcc
     5821 ttgaacactg ccgatgaggt aatagaccac cccgatcatg agaaacttgg ccgcatagtt
     5881 gtcgcctccc ccaccgccca ggatggcgcc ccagcgtccc agcatggtgc cgaagaagtt
     5941 gacgatgacc gtgatgaccg ggatgatcat ccacaagctg tgcacgacgg cgac
//
LOCUS       DASTQY010000319         3063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1086266, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000319 DASTQY010000000
VERSION     DASTQY010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086266"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1761)
                     /locus_tag="VFR82_10470"
     CDS             complement(<1..1761)
                     /locus_tag="VFR82_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEU4502066.1"
                     /translation="MLTSLCPYLDRFTVGFLCTSAYRIALILILTVLGCTKQELPDLG
                     PRLPQTAAMEFSPSLLESKAEYTDNCGHIQIVDIGSTLQDVLLEAANRTFTSVVRPGS
                     GAKPDVVVRVNLVQSTFKLRMDGVYDRAETEIQLGGLVSYVDQAGTALGEQEVQVVQK
                     GRVRIMPVQKNCDYLLDSFIQDAARDFAQKFSVTTRAKLGAPSAPLAAAPAAIPVPVP
                     ARPVTPGVAPLSFKATVLDDNSNLIFEGGERIRVRVDVVNTGAQDLQNVTANLSGSPQ
                     VLAQFPSTTLSAGRLSPGQSRSLEFVATLPQTVQQQKAELNVTVFDPAAGAPPSQALS
                     LSIQPTGTNTDDVDQVPAMSVGFKRPHTYLISIGIGSYRDVQGGARKYAASDAELLTT
                     YFQSLGGLPASNVRLLQDWKALKPDIDEALLDWLPGHMNKDAVVIVYFAGLATVAPNG
                     DVYLVPYDGNTVSASRSYPLKDLDAALGRLKAKQTLFFFDGTLTRLGTDSRSKTVVPQ
                     WNPSGSSTVHLVAMNGTGKAVEDEGHRHGLFTYYLLRALRGESDVNRDGEVTLGEAIA
                     YVGQKVSWASRTQYGQEQRPA"
     gene            1840..>3063
                     /locus_tag="VFR82_10475"
     CDS             1840..>3063
                     /locus_tag="VFR82_10475"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU4502067.1"
                     /translation="MRILGLSNMRDAAAALVENGQIVAAAEEERFVRIKHVTALPVHA
                     VRFCLQTAGIRLADVDAVAVPWKYWQVGRRAALAIGSMLRSPQLFMAKGARSLERLTH
                     EWKELAFLRSSLSRIVDGTPCPAPVFLDHHLCHAASAMLVSPFERAAVLVVDGASESH
                     TTMLGQADRHHIRVLNRIPLPHSLGQFYAAVTAYLGFKPDFDEYIVMGLAAYGEPRVA
                     PVLREKVLRVLPAGEFQLNTRLLDFHLARRRVFSSEFVRLFGAARRPDEAITQRHQDF
                     AASAQLVLEDVLLHLARHLKAVTKEQQLCLAGGVAYNCVANSRIWREAGFQDLFIPPA
                     AGDSGAALGAALWLTHRKDRHFDRMVMRAASWGPKFSEEACRAALEGAGLMVERLADS
                     SLCEKVAGELVAGRLV"
BASE COUNT          593 a          875 c          929 g          666 t
ORIGIN      
        1 cgcaggacgc tgttcctgac catactgcgt tctcgaggcc cagctgactt tctgccccac
       61 ataggcaatc gcttcaccga gtgtcacttc cccatcccgg ttcacgtctg attccccgcg
      121 aagcgcacgc aacaagtagt aggtgaacag cccgtggcgg tgcccttcat cctcgacggc
      181 tttgcccgtg ccgttcatgg cgacaagatg gaccgtcgag cttccggatg gattccattg
      241 agggacgacg gtttttgatc ggctgtctgt tcccagcctg gtcagcgtgc cgtcaaagaa
      301 gaacagtgtc tgtttggctt tcaaacgacc aagagcggcg tcgaggtcct tgagcggata
      361 tgagcgcgac gcgctgacgg tattgccgtc ataaggaacg agatagacgt ctccattagg
      421 cgcgacggtg gcaagaccgg caaaatacac gataaccacc gcgtccttgt tcatgtgtcc
      481 aggcagccag tccaacaggg cttcgtcgat gtccggcttg agggccttcc aatcctgaag
      541 cagacgcacg ttcgatgccg gtaatccgcc caatgattgg aagtaggtcg tcagcagttc
      601 cgcatcggaa gccgcatact ttctcgcgcc tccctggaca tcgcggtatg acccgatgcc
      661 gattgagatg aggtaggtgt gaggccgttt gaaccccacg gacatggcgg gcacttgatc
      721 gacgtcatca gtattcgtgc cggtcggctg gatcgaaagt gaaagggcct gtgacggcgg
      781 tgcccccgcc gcagggtcga acacggtcac gttgagttcg gctttctgtt gctgcaccgt
      841 ctggggaagc gtcgcgacaa attcgagaga ccgggattgc ccgggtgaca accggccggc
      901 tgagagggtc gtactcggaa attgggcgag cacttgaggc gatccgctca ggttcgccgt
      961 gacgttctgc aggtcctgcg cacccgtatt aaccacatcg acgcgaacgc ggatcctctc
     1021 tccgccttcg aatatcaaat tactgttgtc gtccaacaca gtggccttga acgagagagg
     1081 cgccacgccc ggcgtgacag gccgtgccgg taccggaacg ggtatggctg ctggagcggc
     1141 ggcgaggggt gcactggggg caccgagttt cgccctggta gtgactgaaa atttttgggc
     1201 aaaatcgcgg gcggcgtcct gaataaagct gtccaagagg tagtcgcagt ttttttggac
     1261 gggcataatc cggacgcgcc ctttctggac gacttgtacc tcctgttctc caagcgccgt
     1321 gccggcttga tcgacatagg acacgagacc gccaagctga atctcggtct cagcccgatc
     1381 atacacaccg tccatccgca gtttaaatgt ggattgaacg agattcacgc ggactacgac
     1441 gtcaggcttg gcgccggagc cgggccggac cacggacgtg aacgtgcgat tggcggcttc
     1501 caggagcacg tcttgaagcg tgctgccgat atccacaatc tgaatatggc cacaattgtc
     1561 ggtatattcg gctttggact cgagcaacga tggcgagaac tccatagctg cagtctgtgg
     1621 aaggcggggt cccaggtcgg ggagctcctg tttggtacat cccaataccg tcaggatcaa
     1681 gatgagagcg atgcggtagg cactcgtaca gagaaatcca acagtgaacc tgtccagata
     1741 aggacacaga ctcgtgagca tggacgtggc tcaccgtacc ggaatcaatg tctgaaagca
     1801 acttgtcacg cacagccggc tgctttattc taccgcgcca tgcgcatcct cgggttgtcg
     1861 aacatgcgcg atgcggctgc tgctttggtc gaaaacggcc aaatcgttgc tgccgcggaa
     1921 gaggaacggt ttgttcggat caagcatgtc accgccttac cagtccatgc agttcgattc
     1981 tgccttcaaa cagccggaat tcgtcttgcg gacgtcgacg ccgtcgccgt tccatggaag
     2041 tattggcagg tcggccgacg cgctgcgctc gccatcggct ccatgcttcg gtctccgcag
     2101 ctcttcatgg cgaaaggggc caggagcctg gagcgtctga cgcatgagtg gaaagagctc
     2161 gcgtttttac ggtcctctct ctcacgcatc gtcgacggca ccccatgtcc ggctcccgta
     2221 ttccttgatc atcatctctg ccacgccgcc agcgccatgc tggtatcgcc gtttgagcgt
     2281 gctgcagttc tggtcgttga cggagcttct gaatcgcaca ccacgatgct cggacaagca
     2341 gaccgtcatc atattcgcgt tctcaatcgc atcccgcttc ctcactcgct gggccagttc
     2401 tatgctgcgg tcacggccta tctcgggttc aaaccggact tcgacgaata catcgtgatg
     2461 ggattggcgg cctatgggga gccacgcgtt gcccctgtcc tgcgagaaaa ggtcctgcgc
     2521 gtgttgccgg ccggagaatt ccagctgaac acgcgtctgt tggattttca tcttgcgcgc
     2581 cgtcgggtct tttcatcgga atttgtgcgc ctctttggtg cggcgcggcg acctgacgaa
     2641 gcgatcacac agcgtcatca agacttcgcc gccagtgcgc agctggtgtt ggaggatgtg
     2701 ctgcttcacc tggctcgaca cttgaaagcc gtgacgaagg aacagcagct gtgtctggcc
     2761 ggcggggtgg cctacaactg cgtcgccaac agtcgaattt ggcgagaggc cggatttcag
     2821 gacttgttca ttcctccggc ggcaggcgat tcgggcgcgg cgcttggcgc ggccttgtgg
     2881 ctcacacatc ggaaggatcg gcactttgat cgaatggtca tgcgtgcagc ctcgtgggga
     2941 ccgaaatttt cggaggaggc ttgccgggcc gcacttgaag gcgccgggct catggttgaa
     3001 cggctcgctg attcctcgct gtgtgaaaag gtggccggag agttggtcgc cgggcggtta
     3061 gtc
//
LOCUS       DASTQY010000320         5689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_713288, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000320 DASTQY010000000
VERSION     DASTQY010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713288"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..357)
                     /locus_tag="VFR82_10480"
     CDS             complement(76..357)
                     /locus_tag="VFR82_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502068.1"
                     /translation="MSRLNMEPLLSFHDGSHLVMSTQCSKEGNFSCALYTVLISANEG
                     AAFRIISSHSEAQTCLRAQAIAYEHALHLYPQRSDMKQPPYLIWHGPRL"
     gene            complement(1109..1372)
                     /locus_tag="VFR82_10485"
     CDS             complement(1109..1372)
                     /locus_tag="VFR82_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502069.1"
                     /translation="MNQEQFGQFWEQLRTPLKAKWEKVTMEDLDEIQGDLVKFGSVLQ
                     RRYGELHKEEVTTWANRRYSHWTGNYVGYRDAEEFLGLRKGAA"
     gene            3654..4142
                     /locus_tag="VFR82_10490"
     CDS             3654..4142
                     /locus_tag="VFR82_10490"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4502070.1"
                     /translation="MAPQVQPIPPGYHTVSPHLAIRGGVEAMEFYKKAFSAEERGRFM
                     TPDGKHLMHGEVKIGDSIVMLGEENPQKGCPGPQSVGATTVSLFLYVQDADRVFKQAM
                     AAGAKSVMPMADMFWGDRAGTVSDPFGHQWTIATHKEDVPPEEMKKRGQAFFEQMAGQ
                     KT"
     gene            4471..4842
                     /locus_tag="VFR82_10495"
     CDS             4471..4842
                     /locus_tag="VFR82_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502071.1"
                     /translation="MRLRWILPAGLLFLCTSFIAPQPSLADPMDKAAKETEKQKMEME
                     REAQKKRHGMERQAQKDKHATEREERKQKGAMLEDSEKLQREQRKHKEEMAREEEKHQ
                     TEMAREEKKHREEMERGVQSN"
BASE COUNT         1364 a         1471 c         1551 g         1303 t
ORIGIN      
        1 gccgcaatga aagtagctca cttgtatcag ttctatccag tattagcaac acgcatcttg
       61 gtgagagtcg aacggttata gacgtggtcc gtgccaaatg agatacgggg gctgcttcat
      121 gtcgcttctc tggggataca ggtgaagggc atgctcgtag gcgatcgctt gcgccctcag
      181 gcaggtctgc gcttctgaat gcgaggagat aatccgaaag gctgcgcctt cattggcgga
      241 gatgagaacc gtgtagaggg cacaggaaaa attaccctct tttgaacatt gcgtgctcat
      301 gacgagatgc gatccatcgt gaaagctaag cagcggctcc atgttcaagc gactcatagg
      361 tttccttcct gtgacagagg ctgaaagcct ttaacgaggc ggcttccgtt gtttcggcgg
      421 atcgatgcgc tcctcctccg tagattcccc tgccgagagc atatcttcca cctgcgcctc
      481 actgcacgtt agttcgcctg acggtggacg tgacaatctg ctttgctttt cttgtgcaac
      541 ggtttttgca gactttcgca tcgtattacc ctatcccagc aggagacttg acggtgattt
      601 tgaactttca gactggctgt agggagacca gcccgcatag tttacggtcc aatgtaccgg
      661 acgcagtcct gaagccgatc agggaaagaa tatgagaggg tgacatattg cgaccgcttt
      721 tctcacaatg tcggcggtca ggccttccag gaggccgtcg gcaggttgtg ttgagtgagg
      781 gacgtacctt aggcgcatca caggacaatg tcaatgagtt ccatcagtcg gccctactca
      841 tcaatctcca cgatgacatt cgcatcttcc ggaatatctg gagtctcgcc tcttcgagct
      901 tatacgcgac ttttccggct aacttcgccc ttcgattggg gagtttcgat agatggagtt
      961 tctgttgtta gaaacggctc cgcttcagtc gattcacctt gtgagagcac atcatccacc
     1021 tggcattcgc tacatgtcag tccaccactc cggggacgtg gcgagacctg gccctttcct
     1081 tgcgcaacag gcatagcgga atttcttctc atgctgcccc cttgcgcagg cctagaaatt
     1141 cctcggcatc tcggtaacca acataatttc ccgtccaatg agaataccgt cggttagccc
     1201 aggtcgtcac ttcctctttg tgcaattcgc catatcgccg ttgcagtacc gagccgaact
     1261 tgacaagatc gccctgaatt tcgtcgagat cctccatcgt caccttctcc cacttcgctt
     1321 tcagcggagt cctgagttgt tcccaaaact gcccgaattg ttcctgattc atgacgtgct
     1381 ccttggttag agaggttctt tcccggttcg cttgcgacgg accggcgtac tgtacgtggg
     1441 atctgcaaac cgctgagaag cgcctgagac tggtgtggag cagacgcgct aagagaactg
     1501 atggactgag tgagcgggcc agtcaaccga agtcaacagc atgccggtac ctaataaccc
     1561 cgaagcgcgt caagtcaatg agacagcaac gcgggcgaca ccctaaagcg atgaattagc
     1621 acgggtatat ttattattaa tagtctcttt ataaggggag gtcctttaca tgagcacatt
     1681 ctcctcttgg tcaaggtgct ttcacgcgcg ctgccggcga gacacatatc cagcgtccta
     1741 gtacttcgag acatgggctg aaagatcaga tactcgctcc gatgatggac atattcatat
     1801 gtcatataat ggaacagtca ccatttcccc attgaaggag ggttcatggc tcgattaaag
     1861 cggtcaaaaa aggcagggag tccgccgtcc cctgttcgtg gcttcaagct cccgaagata
     1921 gcgaagagaa cagaatcggc tccggacgtg cgagcgaacc gtcggctctc gcagagtcgc
     1981 gctaaaccga ttgctggtca cattcgcgca accacgaagc ggcgtcaggc aaaacgcgat
     2041 agtcgctgag ggactgttct ctattcacgt cgacggttta cttggtgcct ccaacgccag
     2101 gcaagtatca tagttgtggc cgtgagcacg catgagagaa tcatatggca gggatatccg
     2161 gcatggagcc agtttgcctg gctctatctc gcgagctttg tagcgggctc tcgtggtctc
     2221 cgcatcctgt ggcagagcga aacaggatgg gagggctggc tggccggatg cctagcattg
     2281 ctgatgtgcg cggcctgtct acgccaatgg gctcagtatc ttatcatctc gaaccgagtg
     2341 gtgatgcgga acggttatac gggcagagac attcagacgg ttacgctcga cgatattaga
     2401 gaggttaccc tatcgcaagg gccaatcgcg cggttcctca acatcggcac ccttgtagtc
     2461 cactctaaga gtggcagcag ccctttaatg ttgcaagggg tctgcgatcc ggagatcatc
     2521 aagactcgtc ttgaggcctg caggccttga tcggcattgc ctcagcaagc atctcccgag
     2581 tccgtgattg tgccgtgatc gtcttccttc ccttcttctg ctgtcggaag gtcgcgggtt
     2641 gcaacccgcg agagtggtgt ccatcctcga ggacgtcggc tctgagatag gcaaacattg
     2701 ccaatgtgct gggagcgacg tcgggcggat atgtgtcggt gggtcttcga cggtgagcgc
     2761 tccgatcgga tcgcgaacag aaagaaagaa atccccgctt aggtctggcc tggggaattg
     2821 tgccaaaggg tatatctaat tttcctaagg cgttttcagt cgcagtcgag atgctgaatt
     2881 tccgttatgc gcagaacgcg ggcgttccgg tcaagctgca taggaaaaaa ctcggggaat
     2941 acttcggcag gccgacggcc ggatgcgagc gagtgacatc tctctcgccc ttataaccat
     3001 ggacgttgtt cagcaacttt agtgtataat tgaattcggc gtgtgtcttc catccctggg
     3061 tcgattagcg gcatgaagga ggcttgatgt cccaacaaaa gcccatcact gaacgggtcc
     3121 tcgaatttct ccgaggtgcc ccacaatgtg aattcgaggg cttggtagcc cgctaccctg
     3181 aattcacctg gaacgagctc tatctagaag tatcccgctt gaaccggcat ggcttggtaa
     3241 aaattacgcg gggtgttggg atatttacga tccaagaaac ccccgcagta ccctagccct
     3301 tcccacctgc tccagtcgcc actggcgttc aattccgata cacgacagtc ccctgcacgt
     3361 gaggaacaat ctcacaatgg tggttgacca attggcatcg tttatacgat gtcgaatgta
     3421 ccttgtgacg gcacaggtca tcactaggca tgtattgagt tcgtggatac gggttcgtgg
     3481 cggacaggac acaatccatt tgcagtatcg ctgtccatat acctggggaa acccctatct
     3541 ccctatctgt agtttttgac agtacccttt catgagcgtc gctcatatac aattacaatt
     3601 cggtgttttg tccgtcgaat caaaagtcat ccacaatgat gatggaggat agcatggcac
     3661 cccaagtaca accgattcca ccgggctatc acacggtcag tccgcatctg gcgattcgcg
     3721 gtggcgtgga ggcaatggag ttctacaaaa aggcgttcag cgccgaggag cgtggccgct
     3781 tcatgacgcc agacggcaaa catctgatgc atggcgaggt caagattggc gattcgatcg
     3841 ttatgctcgg cgaggaaaat ccccagaagg ggtgcccagg gccacagagc gtgggtgcga
     3901 ccacggtcag cctttttctc tatgtacaag acgccgaccg agtctttaag caagcgatgg
     3961 cagcgggtgc aaaatcggtg atgccgatgg cggacatgtt ctggggcgat cgcgccggca
     4021 cggtgtcgga ccccttcggg caccagtgga cgatcgccac acacaaagag gatgttcctc
     4081 cagaggagat gaagaagcga ggtcaggcat tctttgaaca aatggctgga cagaagacat
     4141 aggttccgtc ctgaaacagc ctcttcgctc ctacgaagaa ggtcacctca agttcatgcc
     4201 atctccaata tatggaatga aacttgttcg gccactaata gcccaggtgc gtgaggcgtt
     4261 cgcgatgcca caaaccatct gtggtgctga aaagcgcgtt ccattttcat gagaattgct
     4321 ggtcctgctt tccactctgc cgtcatgaag gagttccacg aaagggacat tgtgacgaca
     4381 catacttcct agggttcccg ctagcttcac gctagggaac ctggaggtat ggtcgtatgt
     4441 gtgagtacgg acatttttga ggaggcgcat atgagattga gatggatatt gccagcaggt
     4501 ctgctcttcc tttgcacgtc attcatcgca ccacagcctt cccttgcaga cccaatggat
     4561 aaggccgcca aggaaaccga aaaacagaaa atggagatgg aacgggaggc tcaaaaaaag
     4621 cggcacggaa tggaacgtca ggcgcagaag gacaaacatg caacggagcg cgaggagcgg
     4681 aaacaaaaag gcgcaatgct ggaggactca gagaaactcc aacgtgagca gcgcaaacac
     4741 aaagaagaga tggcacggga agaagagaaa caccaaacgg aaatggcacg cgaggaaaag
     4801 aaacatagag aggaaatgga acgaggcgtg cagagtaatt gaacgtgagc tttagtcggc
     4861 gatctctaga ccattggttt cgtcgaggcg ctccgttgga tcaagcgcta attgacggtt
     4921 gtgatgcctc cgactgcaac ctgcattacg aatttcagaa agtccaacca tagacaacct
     4981 aaccgcacag ggtacgacaa accgggaaac tacgacacat gagacccggt ttgttcctgt
     5041 cgtgacgcga acctgcaaca ctcttaactg aatctaatta cttgttccaa tacagaaatg
     5101 tgcgccatga ccacatggtt tgcggtggaa ggcgaagata ttggagactg cataaccact
     5161 cgcgagcggt tgagtcgact cgatttcggt aataccactc ggcgctgcca tgctgccttc
     5221 cacatccgtc gcagtaacgc tcgaggctca tgtaactcct ttctgaacgg cattgatgcc
     5281 ggacagtaca gttcagatgc cgatagctga gagaagcttc cggaggggta ctcaagaact
     5341 gcacgctgta tgaaacgctg gcaagtgaca gcaattggaa aagcgagggt attgtgaggt
     5401 cacgcgaagg atcaaagcgc tcagcgccgt cccaagtgtc ttccagttgc ggccacatca
     5461 aagatggttg tacgtgctcg gcttggttca ggaaggacgg taagttttcg atgatggagg
     5521 attagcggat cgacccactc tccgcagacc atgcaatgca gtgcgtgcaa tggtactccc
     5581 acttcctcga gaacatcctg aagcaggacc ccaccacagc gcccgcattt ctgcataggc
     5641 tgctcttacc ataatggtcg agtgaacgct agatgcgcca cgttcggaa
//
LOCUS       DASTQY010000321         3726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1270315, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000321 DASTQY010000000
VERSION     DASTQY010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270315"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1630
                     /locus_tag="VFR82_10500"
     CDS             <1..1630
                     /locus_tag="VFR82_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502072.1"
                     /translation="ISGAFVLVIAGMMLLNHARPAWAVEEGQFVKKEGKWEYYSGEDP
                     GLKYLYLKGLITQEEYDKGLRVIETKERLSRPNFNVDVNNGLNVRVGDRFLLKLRLLA
                     QARYSYATYNSAWGTVGDSRNPEILGGQVEYRAVRYQSNSSSFSVPRTRLQFLGYAWD
                     PDFRYNVSWAFDQTAWDQEGGSGRARLLDAYIASWHIPWATIQLGQQRVWFNRSQISS
                     IATSTFADNMRVQNAFAANLISNRDVGISILSDEDQYRFNYALGIWNGAGPNLGRDGT
                     SVSQAVLPPTGGGGPPAGTTRTYNYNTRFLNGEMMYTARLLFKIAGNPGYGQGDILNS
                     RTPQAAIGFGYAYNPGQNYLSSIRSDIVDRAYRQGVAKNGNGRLLAGGVYDFQTYEVD
                     LIAKYHGWSLQSEGFYRHQRVRHTDSGTVPFDLSTIPAAQFLGPPVDLGQAWGWYVQA
                     GKYLIPRKLEVAVRYGMMDPSTKQQDDLTKEFGVAINYSFDGTYNNRLVVDYSNITMG
                     SGGRAPDRFPFESQPGFGRDLIENRINVQYQFYF"
     gene            1677..2483
                     /gene="modA"
                     /locus_tag="VFR82_10505"
     CDS             1677..2483
                     /gene="modA"
                     /locus_tag="VFR82_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4502073.1"
                     /translation="MKGLRLLLGLLTLTLLAPHGVLAQPETLTIAAANSVKDALRKML
                     PLFEAEHPHITVRVIYGPSQTLRKQIEEGAPVDVFLPSLFDEIEQLEKKGLVIQGTKR
                     VYAGTSLVLITGTVLPAPIRSIEDLHTVPVHHIAVGDPKTSSVGKVAAQFLKYSKLEP
                     ALKSQYVFGEHSRAVLDLVSKGEAEIGVVYRTDAVADDHVRIVDTAPAESHNPVRYGV
                     AVVWTAKNISGAGDFIEFMLSPPIQAQLKQYGFDQAVSDAGLVRRQEVKP"
     gene            2480..3214
                     /locus_tag="VFR82_10510"
     CDS             2480..3214
                     /locus_tag="VFR82_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502074.1"
                     /translation="MTNRTGINTQRAQWFAPLLFVLVASMTGTGCAGTSDSPPKTIYE
                     SGRNQVRLVKDPDSNSNAHPATLSAAEVGTLLRSVRSWERRNFVHRLFSGEADKTRAF
                     RNEEITVLAPALSKALAQAAPDERAYFHLSHATDQGDEESTTGWLSIRNGTLHLSLSE
                     VHDRHGPEPDISKYDRQMPNVRERSAEFDVTFEPEDYLIKVTSAGKLFAPDQREELQI
                     KYREALAALPTYPGLEPRQPSKPSQP"
     gene            3264..>3726
                     /locus_tag="VFR82_10515"
     CDS             3264..>3726
                     /locus_tag="VFR82_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEU4502075.1"
                     /translation="MEEFEPTGHHIGIVAGTADGASLCYRSLCYEAGRTGRRAQPEIT
                     MHTFPLEWYLNLIERDDWAGVAALMSRSAGKLVQAGAELIICPNNTLHRAFDLAVSAV
                     PWLHIVEPVTAEIRRMRLQRVGLLGTPAVMEGRIYHPQLTQAGIEVVLPEAQ"
BASE COUNT          848 a         1028 c         1047 g          803 t
ORIGIN      
        1 aatttctggt gcgttcgttc ttgtcattgc gggcatgatg ctgctgaacc atgccaggcc
       61 tgcctgggct gttgaggaag gacagtttgt caagaaagag ggaaagtggg agtactactc
      121 gggcgaagat cctggcctca aatatctcta tctcaaaggt cttattacgc aggaagaata
      181 tgacaagggt ctgcgagtca ttgaaaccaa agagcggctt tctcgaccaa atttcaatgt
      241 tgacgtcaac aacggcctta acgtccgagt cggagacagg ttcctgctca agcttcgcct
      301 ccttgctcag gcccgatata gttatgccac atacaattca gcgtggggaa ccgtcggcga
      361 ctcccgaaat cccgaaattc ttggtggaca ggtcgaatat cgcgccgtcc ggtatcagag
      421 caactccagt agcttcagtg tgccgcgaac gcggcttcaa ttcctgggat acgcatggga
      481 tccggatttc cggtacaacg tttcctgggc gtttgatcag acggcctggg atcaagaagg
      541 cggcagcgga agagcgcggc tcctcgatgc ctacatcgcc tcctggcata tcccatgggc
      601 gaccattcag ctcggccaac agcgcgtgtg gttcaaccgt tctcagatca gttcgatcgc
      661 gacctcgacg ttcgcggaca atatgagagt gcaaaacgcg tttgcggcaa acttaatcag
      721 caatcgggac gtgggcatca gcattctcag tgacgaggac cagtaccgtt tcaattatgc
      781 gttgggcatt tggaacggag ccggacctaa tcttgggagg gatggaacgt cggtcagcca
      841 ggccgtgctg cccccgaccg gtggtggcgg tccgcccgcg ggaacgacac gtacgtacaa
      901 ctataatacg cgctttctga acggtgagat gatgtatacc gccagactcc tattcaagat
      961 cgcaggcaac cccggctacg gccagggaga tattctgaat tcacggacgc ctcaggcagc
     1021 gatcgggttc ggctatgcgt ataatcccgg tcagaactat ttgagttcta ttcggtcaga
     1081 tattgtcgac cgcgcctacc gccaaggggt cgcgaaaaac ggcaacggca ggctcctcgc
     1141 cggcggcgtc tacgattttc aaacttatga agtggatctt atcgccaagt atcacgggtg
     1201 gtcccttcaa tcggaaggct tttatcggca tcagcgggtc agacatactg acagtggaac
     1261 cgtgccgttt gatctcagca cgatccccgc tgcccagttt cttggccctc ccgtggatct
     1321 tggccaggct tggggatggt atgtccaggc gggaaaatat ctcatccctc gaaaactcga
     1381 ggtggcggtc agatatggca tgatggatcc gtccaccaag caacaggacg acctgacgaa
     1441 agagttcggt gtggcgatca actatagctt tgacggcacc tataacaacc ggcttgttgt
     1501 cgattactcc aatatcacga tgggaagcgg cggacgggct ccggatcgat tcccgtttga
     1561 aagccagccc gggtttggtc gcgacttgat tgagaatcgc atcaacgtgc agtaccagtt
     1621 ttacttctaa ggatcccatg gccacccatc cacccctaca tgagacgagg acttccatga
     1681 aaggactacg attactgctc ggcttgttaa ccttgaccct tctggcaccg catggtgtgc
     1741 ttgcacagcc ggaaaccttg accattgccg ccgccaacag tgtcaaagac gccctgcgga
     1801 aaatgctgcc gctttttgag gccgaacacc ctcacattac ggttcgcgtg atttatggac
     1861 cttctcagac gcttcgcaag caaattgaag aaggcgctcc cgtggacgtc ttccttccct
     1921 ctctgtttga tgaaatcgaa cagctcgaga aaaaagggct cgtcattcaa ggcacaaagc
     1981 gggtgtatgc cggcacctca ctcgtcttga tcacaggcac cgttctcccc gcccccatta
     2041 gatcgattga ggacttgcac acggttcccg tccaccacat cgccgtcggc gatcccaaaa
     2101 cctcctcggt tggaaaggtg gcagctcagt tcttgaagta cagcaagctt gaaccggcac
     2161 tgaaatctca atacgtgttt ggggagcact cacgtgcggt tctcgacctt gtgtccaaag
     2221 gagaagccga aatcggtgtg gtctatcgca cagatgccgt tgctgacgac catgtgcgca
     2281 ttgttgacac cgcgccagca gaaagccata accccgttcg ttatggcgtg gcagtggtgt
     2341 ggacggctaa aaacatttcg ggggccggtg atttcatcga gttcatgctg tcccctccaa
     2401 tccaggctca actcaagcag tatggattcg atcaggcggt atccgatgcc ggtctagtcc
     2461 ggcgacagga ggtgaaacca tgacaaaccg gacgggaatc aacactcaaa gggcacagtg
     2521 gttcgccccg ctattattcg tcctggtcgc atcgatgacc ggaaccggtt gcgccggcac
     2581 ttccgactcg ccaccaaaaa cgatttatga gtcgggtcga aaccaggtcc gtctggtcaa
     2641 ggatcccgat tcaaactcca acgcgcatcc tgccacgctt tctgctgcgg aagttgggac
     2701 gttgcttcga agtgtccgga gttgggagcg gcgcaatttt gttcaccgcc tattttccgg
     2761 agaggccgat aaaacccggg cctttcgaaa tgaggagata acggtcctcg cgccggcact
     2821 ctcaaaggca ctagcgcagg ccgcacccga cgaacgcgcc tattttcatc tcagccatgc
     2881 caccgaccaa ggcgacgaag agagcacgac gggatggctg tcgattcgga atggaacgtt
     2941 acacctttct ttgagcgaag tgcacgatcg tcacgggcca gaaccggaca tcagcaagta
     3001 tgatcgccaa atgccgaacg tccgggaacg atcggcggag tttgacgtga cattcgagcc
     3061 ggaagattat ctgatcaagg tgacgtccgc tggaaaactg tttgctccgg accagcggga
     3121 agaactccaa atcaaatatc gggaagcgct tgctgcgctt ccgacctatc cggggttgga
     3181 accgcgtcag ccgagtaagc cctcacagcc ttgaatgtgg gcggacctga gacgttcgct
     3241 gtgcaggcta gaggagttac gacgtggaag aattcgagcc gaccggacat catatcggta
     3301 tcgtcgcagg aaccgccgac ggtgcttcgc tctgctaccg cagtctctgt tatgaggcag
     3361 ggcgtaccgg ccggcgtgct cagcctgaaa tcacgatgca caccttcccg ctagagtggt
     3421 atctgaatct catcgaacgg gacgattggg ccggcgtggc agcgctgatg tctcgatcgg
     3481 cggggaaatt ggtccaagca ggagctgagc tgatcatttg cccgaacaat acgttgcatc
     3541 gcgcgttcga cctggcggta tcggcagtcc cctggctgca cattgtcgag ccggtaacgg
     3601 cagagatcag gcggatgaga ctgcaacgtg tgggactgct cggcacaccg gcagtcatgg
     3661 aagggcgcat ctatcatcct caactcacgc aagcgggaat tgaggtcgtt ctgcctgagg
     3721 cgcagg
//
LOCUS       DASTQY010000322         3351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_535056, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000322 DASTQY010000000
VERSION     DASTQY010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535056"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1203)
                     /gene="tgt"
                     /locus_tag="VFR82_10520"
     CDS             complement(46..1203)
                     /gene="tgt"
                     /locus_tag="VFR82_10520"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU4502076.1"
                     /translation="MDFSVGLTDRLTRARLGTLTTAHGRIETPAFMPVGTLGPVKGID
                     PEDLEQLGFRLMLNNAYHLYLRPGHKVVAEMGGLHRFTGWKGAILTDSGGFQIFSLAK
                     LCNVTEAGVSFQSHIDGSAHFISPEVAMEIQEALGADIIMAFDHCVALPADRIAVEEA
                     VIRTTRWAARCQASRHRRAQALFGIVQGGVDEELRTRSARDLVKLEFDGYAVGGLSVG
                     ESKDDMYRMLDVTVPELPVTKPRYLMGVGMPENLIEAVGRGIDLFDCVVPSRHGRTGW
                     LFTTFGRVLIKQARYQRDESPIDPACGCTVCARYSRAYLHHLYNVKEMLASRLNTLHN
                     LWFFSDLMRRMRVAIAQGTFESFRESFYQQQSQEPAGSTASAKTGCDQSSC"
     gene            complement(1225..2976)
                     /gene="argS"
                     /locus_tag="VFR82_10525"
     CDS             complement(1225..2976)
                     /gene="argS"
                     /locus_tag="VFR82_10525"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU4502077.1"
                     /translation="MAHGVVQEKVTSALLGALNGARNKGQLKSTAWPALSLDAPKRPE
                     WGDLASTVAMSLASSEQRAPHDIAQIIVENLPQRDQLFDRVEIVRPGFLNLTVKPALW
                     QETLKEIEGQGALYGTSDLAKGRRALVEYVSANPTGPLHVGHGRGAAVGQALSRLLQA
                     VGYDVVSEYYINDAGRQMKLLGASVLARYREQSGQPGDFPEDGYHGAYISALAERLKP
                     RLDAEGGLSPEQLEERCRTLAYQELLALIRDDLQAFGIDLASWFSEASLLTSQSVERV
                     LDELKSRGLVFEQDGAWWFRSTAFGDEKDRVVKKQDGEYTYLASDIAYHRDKLQRGYD
                     LLIDVWGADHHGYIPRMQAVMQAYGHPKERLQVVLVQLVKLLRAGEEVKMSKRTGEFI
                     TMREVIDEVGADAAKFYFLMRDSKTHLEFDLELAKQRSADNPVYYVQYAHARIASLWR
                     VAASRGIDCPLPSQTNLSLLSDPDELGLIRKLAAYPSTLEGAAVGYEPHRMTYYLQQL
                     AALLHTFYNKHRILPPAADAEVFAAATAGLSADEGAKRESLPPERTAARLALMRGVQQ
                     VIKNGLAVLGISAPDQM"
     gene            complement(3060..>3351)
                     /locus_tag="VFR82_10530"
     CDS             complement(3060..>3351)
                     /locus_tag="VFR82_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU4502078.1"
                     /translation="AGVLTLADLFVGHDSGVTHLAALLGVPTVALFGPTDPARWAPRG
                     PVVTVLQAQPCHCTSWDMVKNCGDKPCLNFSPSAILTACQAARAAGINPRNT"
BASE COUNT          695 a         1032 c          940 g          684 t
ORIGIN      
        1 agggtcccgt cctatctgtt tttcactcac acgccgcatc aacattcagc agctcgactg
       61 gtcgcacccc gtctttgccg atgcggtact gccggccggc tcctgactct gctgttgata
      121 aaaggattcg cgaaacgact cgaatgtacc ctgtgcaatc gccacgcgca tccgccgcat
      181 aaggtcagag aaaaaccaca ggttatgaag ggtattgagt cgtgaagcga gcatctcctt
      241 cacgttgtac agatgatgca ggtacgcgcg agaatagcgc gcgcacacgg tgcagccgca
      301 ggccggatcg ataggcgatt cgtcgcgttg ataccgggct tgcttgatca acacccgtcc
      361 aaaagtggta aacagccatc ccgttctgcc atgccgcgac ggcacgacgc aatcgaacaa
      421 atcaatgccg cgacccaccg cttcaatcag attttcgggc atgcccacgc ccatcaaata
      481 gcgtggcttc gtcactggga gctccggaac cgtgacgtcc aacatccgat acatgtcgtc
      541 cttactttcg ccgaccgaga gccctccgac ggcatagccg tcgaattcca gcttcaccag
      601 atcgcgggcg gagcgagtcc gaagttcctc atccactcct ccctgcacga tgccgaacag
      661 cgcttgagct ctgcgatgcc ggctagcctg acagcgtgcc gcccaccgtg tcgtccgtat
      721 gaccgcctcc tcgacggcga tccggtcagc gggcaaggcg acgcaatgat cgaatgccat
      781 gatgatgtcg gcaccgagcg cttcttgaat ttccatcgcc acttccggcg agatgaaatg
      841 tgcggacccg tcgatgtgtg attggaaact cacacccgcc tccgtcacgt tgcagagctt
      901 ggcaaggctg aaaatctgga agcctccgct gtcggtcaga atggcgcctt tccaaccggt
      961 aaatcggtgg agaccgccca tttcggccac gaccttgtgg ccgggacgca aatacagatg
     1021 gtaggcattg ttcagcatga gccggaagcc cagctgctcc aggtcttcag ggtcgattcc
     1081 cttgacggga cctagcgttc caaccggcat gaatgccggc gtctcgattc gcccgtgagc
     1141 ggtggtgagc gtgccgagtc ttgcgcgcgt caaccgatcc gtcaggccaa ctgaaaaatc
     1201 cacaatccct cttctcgatt cggcttacat ctgatcaggg gcagagattc cgagaaccgc
     1261 aaggccgttc ttgatcactt gctgaactcc tcgcatcaag gctaagcgag cggcggtccg
     1321 ctctggaggc aacgattccc gtttcgcccc ttcatcggcg gagaggcccg ctgtcgccgc
     1381 agcgaagact tcggcgtcgg ccgcaggagg cagaattcgg tgcttgttat aaaacgtgtg
     1441 cagaagcgcg gcgagctgtt gcaggtagta cgtcatgcgg tgcggctcat atccgacagc
     1501 ggcgccttcg agcgtcgacg gataggcagc caacttcctg atcaacccca actcgtccgg
     1561 atcggacaac aatgagagat tcgtctggct gggcaacggg caatcaatgc cgcgcgaggc
     1621 ggcaacccgc cagagactcg cgatcctggc atgggcatat tgtacgtagt agacgggatt
     1681 gtctgccgat cgctgcttcg ccaactcgag atcgaattcc aggtgagtct tcgaatcacg
     1741 catgaggaaa taaaatttgg ccgcatcggc tcctacctcg tcgatgactt cgcgcatcgt
     1801 gatgaactcg ccggtgcgtt tcgacatttt cacttcttcg ccggctcgca ggagcttgac
     1861 cagctgcacc aacacgacct gcaggcgctc tttgggatgt ccataggcct gcatcactgc
     1921 ctgcatgcgc ggaatataac cgtggtgatc agcaccccag acatcgatca acaggtcgta
     1981 accgcgctgg agcttatcac ggtgatacgc gatgtcggag gcgaggtagg tgtattcgcc
     2041 gtcctgcttc ttgaccactc ggtctttctc gtctccaaaa gccgtggagc gaaaccacca
     2101 ggcgccgtcc tgttcgaaca ccaacccacg agatttcagt tcgtccagca cgcgctcgac
     2161 cgattgggaa gtcaacaagg aagcctcgct gaaccaggag gccaaatcta tcccgaacgc
     2221 ttgtaaatca tcgcggatca gtgccagcaa ttcctgataa gccagcgtcc ggcaccgctc
     2281 ctcaagctgt tcaggcgaca accccccttc cgcatcaagc cgcggtttca ggcgctcggc
     2341 cagcgctgaa atataggctc catggtagcc atcctcggga aagtcacctg gttggccgga
     2401 ttgttctcga taacgggcta agaccgacgc tcccaacagc ttcatctgac gtccggcgtc
     2461 gttgatgtag tattcgctga cgacgtcata gccgacggcc tgcagtaggc gagagagggc
     2521 ttgtcccacg gcggcaccgc ggccgtgtcc gacgtgcaat gggccggtcg ggttcgcgct
     2581 cacgtactcc accagcgccc gccgtccctt ggcgagatca cttgtgccgt acaacgcccc
     2641 ctgcccctct atctctttga gcgtttcctg ccagagcgca ggcttgacgg tgagattcag
     2701 gaatccgggg cgaacgattt ccacccggtc gaagagttga tcccgttggg gaagattttc
     2761 aacgatgatc tgcgcgatgt cgtgaggcgc tcgttgctca gaggacgcaa gggacatggc
     2821 gacggtcgag gcgaggtctc cccattcggg ccgcttcggc gcatcaaggc tgagcgccgg
     2881 ccaggccgtg cttttcaatt gtcctttgtt cctggctccg ttcagggcgc cgagcaacgc
     2941 actggtcacc ttttcctgca ctacgccgtg ggccaaaatg tctcctaagt cctagcattg
     3001 aaaagagaaa attaactcgg cactctatca tagggtggaa ttgcgagaca aggcacaggt
     3061 caggtatttc gtgggtttat gccggcagct cgagccgcct gacaggcggt taaaatcgcg
     3121 ctcggcgaaa agttcaggca cggcttgtct ccacagtttt tcaccatgtc ccatgacgtg
     3181 caatggcaag gctgtgcttg taagaccgtc accacaggcc ccctcggtgc ccatcgcgcc
     3241 ggatcggtcg gcccgaacag ggccacggtc ggcactccca gcaaggctgc gaggtgagtg
     3301 actcccgagt catgtccgac aaacagatca gccagcgtga gcaccccggc c
//
LOCUS       DASTQY010000323         3464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_629031, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000323 DASTQY010000000
VERSION     DASTQY010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629031"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFR82_10535"
     CDS             <1..310
                     /locus_tag="VFR82_10535"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEU4502079.1"
                     /translation="GALSAAEDTGLIATVVPNVALSGWRGFVSFDVGVGLGFLGREKY
                     GTQEFGGHVQFVLTTALQIHPISHAFAGFRLQHFSDAGIYGSDSLGFDLYIIEVGYRF
                     "
     gene            complement(612..1496)
                     /locus_tag="VFR82_10540"
     CDS             complement(612..1496)
                     /locus_tag="VFR82_10540"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU4502080.1"
                     /translation="MSTTIESIIRLLTTTATKWWDDNPWRLSAALSYYTLFSLAPLLT
                     LAVAVAAVVVDEHVVQGELRHQLEDLIGEHGANAMTPMLHSAGHPVHGTIATLISFST
                     LFIVSMGMFSELQDALNFIWRIPCRVRSAALWGLLRTRLFSFLLVIGTGFLLIAALVL
                     NVVMAAAGTFMKSALSQPQVLMMVIDIMGSPIVMAILFTLMFKMLPAGHIAWQDVWIG
                     GIATAALFTLGQWAIEIYLRGPAMTSMYGAASSLMAILVWVYYSALIFFLGAEFTYVY
                     AHQYGSRRPAQSRVSRPC"
     gene            1619..2095
                     /locus_tag="VFR82_10545"
     CDS             1619..2095
                     /locus_tag="VFR82_10545"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEU4502081.1"
                     /translation="MRTQSRTPISILQSAGVGGLILIALVGTAGSSQASQPGLHVEIL
                     GIRNSIGAVACALFQGPEGFPTEFLRFASKMMMVKVRATKATCDFADIAPGTFALAVI
                     HDENRDGKLATNVMGIPQEGYGFSNDAKGSGGAPSFEAASFSYNGQSLNMTIALQY"
     gene            complement(2405..3283)
                     /locus_tag="VFR82_10550"
     CDS             complement(2405..3283)
                     /locus_tag="VFR82_10550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4502082.1"
                     /translation="MNHLKNNGSMTADVHTAKEALLDSQDTGINAGRAADTTLQQWNR
                     LLEQQLSECTQELGRSRVDLRSLATALDAAEQRERTHLATELHDYLAQLLVLGRMKIG
                     QLQRLSVPSAGDAMVREVDEVLSQALQYCQTLMAELSPPILHEQGLVAGIRALATQMK
                     RHELEVRVEIDPMDECRLPISCTVLLFRSVRELLINTAKHAAVKQATVRLTCDQGLLQ
                     IVVRDESGFDLAVSQGPSLAGTGNILSSKIGLLTIRERMKALNGRFDFVSAPKQGTTA
                     TLTMPISPRNASMLSA"
BASE COUNT          780 a          951 c          945 g          788 t
ORIGIN      
        1 gggggccctc tccgccgcag aggacaccgg gctaattgcg actgtcgtcc ctaatgtggc
       61 cttgagcggc tggagagggt ttgtatcgtt tgatgtgggg gttggcttag ggttcctcgg
      121 tcgggagaaa tacggcactc aagaattcgg ggggcacgtc caatttgtgc tgaccactgc
      181 gcttcaaatc catccgatct cgcatgcgtt tgcagggttt cgccttcagc acttctcaga
      241 cgccggcatc tatggttctg attctctcgg tttcgacttg tacatcatcg aggtcggcta
      301 taggttttga gtgtttggcg catgggaatt cctaatgttc tatgctctcg gcgatcgttt
      361 gatcaacaat gggtgctcgt cattaaccac ggtgtttggc agaacttcat gtccgatcgg
      421 tttgaagcct ctgactcttc tgtaagatct gcaatgtttt aatacctgcc ttgataatat
      481 atgcaagcta gccctcttgg agtcgttgtc atgatctgtc ctccactctt tcaccgcagt
      541 gtcaccgagc acaggtcatc ctgccaaacc cagccgggca acgagtaaaa accgccggat
      601 atggtggaaa atcaacaggg tcggctaacc cgtgattgtg caggacggcg tgagccatac
      661 tgatgcgcat acacatacgt gaactcagct cccagaaaga aaatcaaggc agagtagtat
      721 acccacacca agattgccat caatgaagag gcggccccgt acatcgatgt catcgcgggc
      781 cctcgcagat agatttcgat tgcccattgt cccaacgtga acagcgcggc agtagcaatt
      841 cctccaatcc agacatcctg ccaggcgata tgtcctgccg gtaacatttt gaacatgagc
      901 gtgaacagaa tggccatgac aatgggtgat cccatgatgt cgatcaccat catcaagacc
      961 tgcggctggg acagcgcgct cttcatgaat gtccccgcag cggccataac gacgttcagt
     1021 accaaggcgg cgatcagaag gaaccctgtc ccgatgacca gcagaaatga gaagagtcgc
     1081 gttctgagca gtccccaaag ggcagcactc cgtacccggc acggaatccg ccatatgaaa
     1141 ttaagcgcat cctgcagttc actgaacatg cccatggaaa cgatgaagag ggtactgaaa
     1201 ctgatgaggg tggcgatggt gccatggaca gggtgaccgg cgctgtgcaa catgggcgtc
     1261 atcgcattgg ctccatgctc tccgataaga tcttccaact gatgacgcaa ctcaccttgc
     1321 acgacatgtt catccacgac aactgccgcc acggcgacgg ccagcgtcaa gagaggggcg
     1381 agggagaaca gcgtgtaata cgacagggcg gcactcaacc gccagggatt atcatcccac
     1441 cacttcgtcg ccgtggtcgt gagcaagcgg atgatcgact cgatggtcgt gctcattcaa
     1501 gtggatccta cggccagtgt cggcccgcca tcattgtacc tagggctatg cagggtggac
     1561 gggagtacac agtcatagag aatacttaga cggacgttac catgcacgag tcgccctgat
     1621 gcgcacacaa agccggacgc cgatatccat tctacagtca gctggtgtgg ggggacttat
     1681 actcatcgct cttgtcggta cggccggctc ctctcaggca tctcaaccgg ggttacatgt
     1741 ggagatcctc ggcatccgga atagtatagg agcagtggca tgcgcattgt tccaaggacc
     1801 ggaaggattt cctactgagt ttttgcgctt tgccagcaag atgatgatgg tcaaagtccg
     1861 tgctaccaag gccacttgtg actttgcaga catagcgccc ggcacatttg cacttgcggt
     1921 gattcatgat gaaaaccgag acggcaaact cgccacaaac gtgatgggga tacctcagga
     1981 aggatatggc ttttcgaacg atgcgaaagg ctcaggaggg gcgccctcgt ttgaggcggc
     2041 cagtttttca tacaatggac aaagcttaaa tatgacgatc gccttacagt actgattcgg
     2101 tccccagcgc agtcagggtc ctgctctttg gtgccaacct tttagttgtc gcaggaatac
     2161 gactgctcca atgtcggcca cgtgaggaac cgaccatttt ggcaggatgg cggaggcgat
     2221 tggcacgaac cacggagtcg tcaatgcacc cacaattaac gcatcgtgtt gccaggcaga
     2281 tgcgccctct ctcatctctc agctctccga aattttcggg gatgagcaga ccatggcagc
     2341 gttgacataa catagatgct tctcctctcc ttcatacctc cgcccaccgc ccgcaggcct
     2401 tctctcatgc ggagagcatt gaggcgttgc gcgggctgat cggcatggta agggtcgccg
     2461 tggtgccctg cttgggtgcc gatacaaaat caaagcgacc gtttaaggct ttcatgcgtt
     2521 cccggatcgt gaggagaccg atcttcgaag agaggatgtt ccccgttccg gcaagcgatg
     2581 gcccttgcga gaccgcgaga tcgaaaccgc tttcgtcacg caccacgatc tgcaggagcc
     2641 cctggtcgca ggtcaggcgg accgttgctt gttttacggc cgcatgcttc gccgtgttga
     2701 tcagcagctc ccgcaccgag cgaaagagca ggacggtaca actgatagga aggcggcact
     2761 catccattgg atcaatttcc actcttacct ctagctcgtg gcgcttcatc tgtgtcgcga
     2821 gcgctcgaat ccccgcgacc agtccttgtt cgtgcaggat aggtggactc aactcggcca
     2881 tcaacgtctg gcaatactgt aaggcttggc tcaacacctc atcgacttct cggaccatcg
     2941 catcgcctgc tgaaggcaca gagagtcgct gcagctgtcc gattttcata cggccgagca
     3001 cgagcaactg cgccagatag tcgtgcaatt ccgttgctaa gtgcgtgcgt tcacgctgct
     3061 ccgcggcatc gagcgcggtc gccagggacc ggagatccac ccgggaacgg cctaactcct
     3121 gagtgcactc gctgagttgc tgttctagca atcggttcca ctgttggagc gtggtatctg
     3181 ccgctcgccc cgcgttgatc ccggtgtcct gagagtcaag tagcgcttct ttcgccgtat
     3241 ggacgtctgc cgtcatgctt ccattattct ttagatggtt catatgcggc tcctttggta
     3301 tgtctcaact atcgagatat aacaaaacaa gtaaggatcg aagggaagca ctagttagaa
     3361 ccctcgtgat catgccagca tgttagagcg gcgggcgaag ccgaactgtc tcaaacagac
     3421 catacggttg gttcggataa atccgcctct taaataggtt tcac
//
LOCUS       DASTQY010000324         4484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1439209, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000324 DASTQY010000000
VERSION     DASTQY010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439209"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..3390)
                     /locus_tag="VFR82_10555"
     CDS             complement(124..3390)
                     /locus_tag="VFR82_10555"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU4502083.1"
                     /translation="MLTRSALKNPYAVFALCMIALVLGAVSYQKMRVDIFPEIKIPTI
                     LVTTFYRGMSPGEMEGAITLRMEQRFVEASYVEHIESQSLAGMSYIKVFFQPEYSIDA
                     AQSELTSLAYSIIRLLPPGVYPPSVYKFGVASLPVGFLSISSESLGAKDIRDLAYFTV
                     RNQIANIPGISFGPPLGGKVRQITVFMDQQRMLARGISPSDVVKAINAQSAIIPAGDI
                     KIGDLDYYVYSNSLVGVVDKINDIPIKVVNGTPILVRDIGTAADSAAIQTSIVRVNGR
                     EATYLPITRQEGANTLEVTDGVRAKLHKLTEIPAGTTVKFIYDQSLYIRQAIANLQKE
                     GLLGAGLAGLMIFLFLRSMKAALVVGLAIPLSLTAALVALFLSGQTVNIMTLGGLALV
                     IGTLLDNNIVVQENLHRHLEMGKDGRSAAEDSATELTLPIFVATLCILIVYLPIIFFA
                     GIVKYLFVPLAMTVAFAMLADYAVSMTVTPVVLARLYSRGHGNGSEDDASAAHGWFRF
                     VLSAYEMVLRAGLRLKWLVLILALTALLVASVTLLPRLHTEFFPKVDAGNFTMLVTAP
                     EGSRIEKTTAIVADIERLVQETVPRQELEEVVSNTGLYFGDAARFAPNTGNHTAFVLV
                     NLVSGHTGRTDSYIDVLREKARKSLPGVEITFQTGGIISDVLNFGLRAPIDIQIKGPS
                     LDLIRPVAEHIRQEVAQVANTTDVRIKQGKSYPELHVNVDRTKAAYYGLTQDKVIVDV
                     ITGISSNLALSPNYWLDPKTANGYFLLAQYPEQSLTKTEDLLNIPVIGARTPLNPTSA
                     LTTAGSSGSTIALQNTPFAGRNLDLSAGFFASDERRGPPVLLRDVASLEIKTGPDSVD
                     HYDLSRLLDVLVTPVGNDLGRVATDIEAVLQRIVLPKDVTIELKGEVANMRAAFSNFS
                     LALPLAVLLIYLVMVGLFRSMVDPLIILVAVPLGWIGTIVMLHLTDTSINVESMIGTL
                     MMMGIVVSNSILLVDFANRMVRAGASADRAVLEAGRRRIRPIVMTALATILGLLPLAL
                     GFGEGNETMVPLARAVVGGLAVSTVMTLLVVPVMHALVLSRRIHIERSLTPAASSEEI
                     "
     gene            complement(3384..3815)
                     /locus_tag="VFR82_10560"
     CDS             complement(3384..3815)
                     /locus_tag="VFR82_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502084.1"
                     /translation="MHRHASVLLIDDSPGERELFREALMRTDLDVALYSEQDAEAALH
                     FLKHRPELPSLILLDWHLRHERGDVFLKRLRSERRFAAIPVVAFTTSDEVADLSTAYA
                     NSVNGYVVKPDTFEDLVQCVEDICRYWLSRNRTPYPVETPC"
     gene            complement(4105..>4484)
                     /locus_tag="VFR82_10565"
     CDS             complement(4105..>4484)
                     /locus_tag="VFR82_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU4502085.1"
                     /translation="TQGFSPVTSQAAMSALGVGTQVGVLLPFSRAHESEADYIGLLLA
                     ADAGYDPQEAVRVWQRMQQSSDGKQPQEFLSTHPGHETRIKRLTEKMPEALALYNQVQ
                     KAPVAQLPPVGSPGTAAPLAQQQ"
BASE COUNT          983 a         1225 c         1368 g          908 t
ORIGIN      
        1 accggttgca tatcggcctg aagaggcggc cgctgggcct tgcctgatgg ttcttctggc
       61 tggcatccga tggaccagag cagcagagcc agaatgatca tgaaggcgcg tgtcggcatg
      121 agcctagatt tcctccgatg aagcggcggg agtcagtgaa cgttcgatgt ggatgcgccg
      181 ggacagcacc aacgcatgca tgacggggac aacgaggagc gtcataacgg tcgagacggc
      241 aaggccgccg accaccgcac gggccaacgg taccatcgtc tcattgcctt ccccgaatcc
      301 gagtgccaag ggaagcaggc cgaggatcgt cgcgagagca gtcatcacga tcggtcgaat
      361 acgccggcgt ccggcttcca gaacagcccg gtcggcggag gcccccgctc ggaccatccg
      421 gttcgcaaaa tccacaagca gaatgctatt cgagactacg atgcccatca tcatgagcgt
      481 gccaatcatc gattcgacgt tgattgacgt atcggtcaga tgcagcatga cgattgtgcc
      541 gatccaaccc aatgggactg cgacgagaat gatgagcgga tcgaccattg agcgaaacag
      601 gccgaccatc acaagataga tcaacaggac cgcaagaggc agcgcgagcg agaagttgct
      661 aaaggcggcg cgcatattgg ccacttcccc cttcagttcg atcgtcacgt ccttcggaag
      721 cacaattctc tgcagcaccg cctcgatatc tgtagcgaca cgcccgaggt cattgccgac
      781 cggtgtcacc aacacgtcga gcaaacgcga caaatcatag tggtccacac tgtccgggcc
      841 tgtcttgatc tccagcgagg ccacgtcgcg gaggagcacc ggtggccccc gacgttcgtc
      901 ggaggcaaag aaaccagcgg acaagtcgag attgcgcccg gcaaaaggcg tattttgaag
      961 cgcgatggtc gagccgctgg atccggcggt ggtgagggct gaagtcggat tgagaggggt
     1021 ccgggcgccg ataaccggaa tattgagcag atcttcggtc ttggtcagtg attgctcagg
     1081 gtactgggcc aagagaaagt agccgttggc cgtcttggga tcgagccagt agttaggact
     1141 cagggctaag tttgagctga tgcctgtgat cacatctacg atgaccttgt cctgcgtcag
     1201 tccgtaatag gcggcttttg tccgatccac gttcacgtga agctccggat agctcttgcc
     1261 ttgcttgatg cgcacgtccg tcgtattggc gacttgggcg acctcctgac ggatatgctc
     1321 tgcaaccgga cgaatgagat ccaaggacgg tcccttgatc tgaatgtcga ttggggcacg
     1381 caggccgaag ttcaacacgt cgctgatgat gccgcccgtt tgaaaagtga tttccacccc
     1441 gggaagtgac ttgcgggctt tttctcgcag gacgtcaatg tacgaatcgg tccggcctgt
     1501 atgcccggac accagattga cgagcacgaa ggccgtgtga tttcccgtgt tgggtgcaaa
     1561 acgggccgca tcgccaaaat agaggccggt attggagacg acttcttcaa gctcctgcct
     1621 gggaactgtc tcttgcacga gtcgttcaat gtcggccacg atggcggttg tcttctcaat
     1681 tcgagagccc tcgggggcgg tgacgagcat cgtaaagttt cctgcgtcga ccttcgggaa
     1741 aaattccgta tggaggcggg gcagcagagt gacgctcgcg accagcagag cagtcagcgc
     1801 caagatcagt accaaccact tcagccgcag gcccgcgcgg agtaccatct cgtatgccga
     1861 caagacgaag cgaaaccagc cgtgggcggc cgatgcgtcg tcctccgacc cgttcccatg
     1921 cccacgggaa tacagccggg ccagcacgac gggcgtcacc gtcatcgaca cggcgtagtc
     1981 tgcgagcatg gcaaacgcca ccgtcatggc caggggcacg aacagatact tgacgattcc
     2041 tgcaaagaag atgatcggca ggtagacgat cagaatacag agcgtggcga cgaaaatagg
     2101 aagcgtcagc tcggtggcgc tgtcctcggc ggctgaccgg ccgtcttttc ccatttccag
     2161 gtgacgatgg aggttctcct gaaccacgat attgttgtcg aggagggtgc cgatgacgag
     2221 ggcgagaccc ccgagcgtca tgatgttgac agtctggccg ctgagaaaga gggcgaccag
     2281 cgcagcggtc agcgagaggg gaatggcgag ccccaccacc aatgccgctt tcatgctgcg
     2341 caggaataaa aagatcatca agccggcaag cccggcaccc aacaatcctt ccttctggag
     2401 gttagcgatg gcttgccgga tgtacaacga ttggtcgtaa ataaatttca cggtggtgcc
     2461 ggcgggaatt tccgtcaact tgtgcagctt cgctcgaacg ccatccgtca cttccagagt
     2521 attcgccccc tcttgtctcg tgatcggcag atacgtcgct tcacggccat tgacgcggac
     2581 gattgaggtt tgaatggcgg cgctgtccgc cgcggtcccg atgtcgcgaa cgagaatcgg
     2641 cgtgccgttt acaactttga tcggaatgtc attaatcttg tcgaccacgc cgacgagact
     2701 gttcgaatac acgtagtaat ccaggtcccc gatcttgatg tctccggcag gaatgatcgc
     2761 gctttgcgcg ttaatggcct tcaccacgtc tgacggcgaa atgccccgag ccagcatccg
     2821 ctgctgatcc atgaagactg tgatctgcct gactttgccg cccaatggag gaccaaagga
     2881 gatgccggga atgttggcga tctggttgcg cacggtaaaa taggccaggt cgcgaatgtc
     2941 tttcgcgccg aggctctcgc tgctgatgga gagaaagccg accggtaggc tcgcgacgcc
     3001 gaacttgtag acggaaggcg gatagacgcc gggcggcagc agacgaatga tgctgtaggc
     3061 caaactggtc agttctgatt gcgcggcatc gatggaatac tccggttgaa agaagacttt
     3121 gatataactc atgccggcga gcgactggga ctcaatatgc tccacgtaac tggcttcgac
     3181 gaaccgttgt tccatccgga gcgtgatggc tccttccatc tcgccgggac tcatgccacg
     3241 gtagaaggtg gtgacaagga tcgttggtat cttgatttcc ggaaaaatgt cgacacgcat
     3301 tttttggtaa gagacggccc cgagcaccag agcgatcatg catagcgcaa aaacggcgta
     3361 agggtttttc aatgcggatc gtgtcagcat ggcgtttcca ctggataggg cgttcgattt
     3421 ctgctcaacc agtaccggca gatatcttcc acgcactgca ccaggtcttc gaaagtgtcc
     3481 ggcttcacca catagccatt gacggaattc gcgtaagcgg tggaaaggtc ggcgacttcg
     3541 tcggaagtcg taaaggcgac gacgggaata gctgcgaacc gtcgctcgga gcgaagacgt
     3601 ttgagaaaga cgtcgccccg ttcatgccgg aggtgccaat cgagcaggat caacgaaggt
     3661 agttcaggcc ggtgtttgag aaagtgcagc gctgcctcgg catcctgttc ggagtacagg
     3721 gcgacatcga ggtcggtgcg catgagcgcc tcgcggaaaa gttcgcgttc gccggggctg
     3781 tcgtcgatca ataagacgga ggcgtggcgg tgcatcgaga agcagttcag cagagatcgt
     3841 gccttggcgg gactgaagga agaagcgatg acttttttcg agagatcaat tacttaactg
     3901 cctcacctca tgaaactggg cgacaaggcc attgagacag gcctggagaa cttccagagc
     3961 cctctggaag gtttccatat cagcagcgga agaggaagcc cgccgatggg tgctgtgttg
     4021 aggcggtcat tctaatgctt caggttcggt cgaccgctgg gggcaggacg gcgccacgag
     4081 aatcccgacg cccgggactt cttcttactg ttgttgtgcg agtggggctg cagtccctgg
     4141 ggagccgacc ggtggaagct gcgcgaccgg cgccttttga acttgattgt agagcgccag
     4201 agcttcgggc atcttttcgg tgagccgttt gatcctggtc tcgtgtcccg ggtgggtcga
     4261 gagaaattct tgtggttgtt tcccgtcaga ggactgctgc attctttgcc acactcgcac
     4321 cgcctcctgc gggtcgtagc cggcatctgc cgccaacagc aacccgatat agtccgcttc
     4381 ggactcatgg gcacgactga acggtaagag cactcccacc tgtgtgccga cgcccaatgc
     4441 ggacatggca gcctgacttg tcaccggact gaatccttga gttc
//
LOCUS       DASTQY010000325         6215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1638664, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000325 DASTQY010000000
VERSION     DASTQY010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638664"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFR82_10570"
     CDS             <1..217
                     /locus_tag="VFR82_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502086.1"
                     /translation="VAVDSVPQPFMGTVSYISPRAEYTPPVIYSQESRDKLVFMIEVV
                     FDPQSAVNLNPGQPVDVRFESSSGGGR"
     gene            214..1143
                     /locus_tag="VFR82_10575"
     CDS             214..1143
                     /locus_tag="VFR82_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4502087.1"
                     /translation="MTGQALAIDVHGMTKRFGERTVVDHIGLQVRRGEIFGFLGPNGS
                     GKTTFIRMLCGLLRADEGSGTCLGYDVVRDSESIKAEVGYMTQRFSFYEDLSIAENLD
                     FVARMFGVKNRREAVARSIERLGLGGRQGQLAGQLSGGWKQRLALAACLIHQPQLLLL
                     DEPTAGVDPKARREFWEQIHELAADGLTFLITTHYMDEAERCHRLAYIAYGRLLTHGT
                     VTEVIAQARLTTWSVSGPNLHHLAEELRKHSGVRQAVAFGNRLHVSSDDAKALEAAIL
                     SFRKPPYEWRPISSGLEEVFIHLMNRTSDNFTS"
     gene            1155..2282
                     /locus_tag="VFR82_10580"
     CDS             1155..2282
                     /locus_tag="VFR82_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4502088.1"
                     /translation="MFSVPRLWAIVVKEFIQMRRDRVTFGMMIGVPLIQLVLFGYAIN
                     ADPKRLPTAVLMADYGPQGRTVLHAIRNSGYFEFKRELTSEQEGRDVLARGDVQFVVN
                     IPQGFTRDLLRGERPAILIEADATDPAATSNAIGSLRMVLARALQHDLKGPLAFLAGR
                     EDPIELRVHAQYNPEAITQYNIVPGLMGVVITMTMVMITGLAITRERERGTMENLLSM
                     PTRPFEVMVGKIIPYILVGYVQLGLILLAARVLFEVPMHGNLGLLLLVAFLFITANLA
                     MGITFSTLAENQLQAVQLSFFFFLPSLLLSGFMFPFRGMPEWAQAVGELLPLTHFLRV
                     VRGILLKGNGLYDIVLQLWQIALFAVVALAVGIKRYRQTLD"
     gene            2354..2803
                     /locus_tag="VFR82_10585"
     CDS             2354..2803
                     /locus_tag="VFR82_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU4502089.1"
                     /translation="MKAFFRTDEGWTGFILRTTLGLVMLPHGAQKLLGWYGGLGFSGT
                     LGLFTEKMGIPLSIAFLVIVAESFGSLALLAGFLTRFAAASLAVIMLGAIVMVHLPQG
                     FFMNWFGQQQGEGFEYHLLVLGIAAALLVTGGDRWSVDRILAERIAR"
     gene            2821..3720
                     /locus_tag="VFR82_10590"
     CDS             2821..3720
                     /locus_tag="VFR82_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU4502090.1"
                     /translation="MTTDVVTKKEVLGVYQPGSAHMVGDGFPVRNLFPSNELDREVDP
                     FLMLDYAGPRNFPPTEHPRGVGEHPHRGFETVTIMYEGAVAHRDSAGNAGVIGPGDVQ
                     WMTAASGIVHEEMHEAHWAKQGGTFRAIQLWVNLPQAHKMSAPAYQTLVNDDIPAVDL
                     EGGSGRLRVIAGTFRGHKGPARTFTPVELYDLQLRAGARFELTLPEGHNTSVFVLEGR
                     VSVNGSAAAGEAELVVSKGQGSLVRLEVIDDSRLLIMSGKPINEPIARYGPFVMNTRA
                     ELVQAVEDYQRGRMGHLSPLDAG"
     gene            3872..4537
                     /locus_tag="VFR82_10595"
     CDS             3872..4537
                     /locus_tag="VFR82_10595"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEU4502091.1"
                     /translation="MDHQNTRITVEVYSDVVCPWCYVGKRRFERAQSQLPTTATQVIW
                     RPFQLNPTMPCEGMDRRTYLEAKFGSQDSYHRLEEQVAAAGASEGISFAFERMRRTPN
                     TFVAHRLIWYAGQHERQNLVVDALFKAYFEQGADIGSHAVLTDLAASAGLDAASFLQT
                     EEGAADVKAQEAVGRRLGIRAVPCFVFNKSDGISGAQPVDVFVGAIKNAHARDAEDQL
                     TRK"
     gene            4541..4975
                     /locus_tag="VFR82_10600"
     CDS             4541..4975
                     /locus_tag="VFR82_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4502092.1"
                     /translation="MSVQVYGCNHVVIEVTDAKKAVQFYSDVFGLKMLRGGEGAAWCK
                     LGEHQFMAIFEVEKLQPDRVKHFGLMVRDPEQINTVRRKLTSKYKLKLHPDFRCDFRD
                     PWGNRIQVGDLSDESLVWLLPYQEVQKAGLIFRSASKRRRRD"
     gene            4979..5380
                     /locus_tag="VFR82_10605"
     CDS             4979..5380
                     /locus_tag="VFR82_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502093.1"
                     /translation="MTRRSHTATQLLHLSKALLRLHKTLLDGERAAYERLHGPISSNG
                     AFLQLVLGHPSFAWLRDLSRSMAHLDELAEAKEASPAEALHAQTASLRALLTPMKDGE
                     EFHRRYHDALERDPDAALAHAAVIELLSDTR"
     gene            5395..5841
                     /locus_tag="VFR82_10610"
     CDS             5395..5841
                     /locus_tag="VFR82_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4502094.1"
                     /translation="MKAQYLGHVVFYVKDLEQSLAFYRDLLGFKEVGRIFGGAAAALT
                     SGRTHHELLLIQVGDAPPPPSGRRRGLYHIGIKVGDSLDELRSAKRELDQAGIAIDGM
                     SDHTVSQSLYLRDPDGNEIELYVDADEAVWKADPAAVLSPIKSLRL"
     gene            5884..>6215
                     /locus_tag="VFR82_10615"
     CDS             5884..>6215
                     /locus_tag="VFR82_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU4502095.1"
                     /translation="MDQSASPCETLGERYQALLEVAEAISAHRDVHELFRDLARRLPR
                     VVQVNFVALSLHDPAGNRMRLQTIQANVPADLVGGHEEPLEETPAGSVWQSQQPILVP
                     DLDGELRWP"
BASE COUNT         1266 a         1732 c         1899 g         1318 t
ORIGIN      
        1 ggtcgccgtg gatagtgtgc cccaaccgtt catgggcacg gtcagctaca tctcgccgcg
       61 cgcagagtat acgccgccgg tcatttatag tcaggaaagt cgagacaaat tggtattcat
      121 gattgaagtg gtgtttgatc ctcagagcgc agtcaatctc aatccgggcc agccggtgga
      181 tgtccggttt gagtcttcct caggaggagg gagatgacgg gacaggcact cgcaatcgac
      241 gtgcatggca tgaccaaacg gttcggcgag cgcacagtgg tcgatcacat agggcttcaa
      301 gtgaggcgcg gggagatttt cggttttctc ggaccgaacg gcagcggcaa gaccacattc
      361 attcggatgc tctgcggtct cctgcgggcg gacgagggca gcggcacctg cttggggtac
      421 gacgtcgtac gcgacagcga atcaataaag gccgaagttg gttatatgac tcagcggttc
      481 agtttctacg aagacttgag catcgccgag aatcttgatt tcgtcgcacg gatgttcggc
      541 gtgaagaacc gtcgtgaggc cgtcgcccga agcatcgagc ggctcggtct cggcggccgc
      601 caggggcagc tggccggaca gttatcgggc ggctggaagc agcggcttgc actggcagcc
      661 tgcctgatcc atcaaccgca gctgctgttg ctcgatgaac cgactgccgg agtggatccg
      721 aaagcgcggc gggaattctg ggagcagatt cacgaactgg ccgccgacgg cctgacattc
      781 ctgattacga cccattatat ggacgaagca gaacgatgtc accgcctggc ctacatcgcc
      841 tacgggcgat tgctgacaca tgggacggtg acggaggtca tcgcccaagc gcgtctcacg
      901 acctggtcgg tcagcggacc aaatctgcac cacctcgccg aggaactgcg gaaacattcc
      961 ggcgtacgac aggcggtggc tttcggcaac cggttgcacg tcagcagcga tgatgccaag
     1021 gcgctcgagg ctgccatcct atcgtttcgc aagccaccct acgaatggcg tcctatcagc
     1081 tcaggactcg aggaagtgtt tatccatctg atgaatcgga cctctgacaa ctttacctcg
     1141 taaggaggtc gccgatgttc tcagttcccc gcctctgggc gatcgtcgtg aaagaattca
     1201 tccagatgcg ccgtgaccgt gtgacgttcg gcatgatgat cggcgtcccg ctgatccaac
     1261 tggtgctgtt tggatatgcc atcaatgccg atcccaagcg cctcccgaca gccgtcctga
     1321 tggccgacta tggcccacaa ggacggactg tactccatgc gattcggaac agcggctatt
     1381 ttgagttcaa gcgggagttg acgagcgaac aggaaggtcg tgacgtcctc gcccgggggg
     1441 acgtgcagtt cgtcgtgaac attccacagg gtttcacgcg cgatctgttg cgcggagaac
     1501 ggccggccat actgattgaa gccgacgcga ctgacccggc agccacgagt aacgccatcg
     1561 ggtccctacg gatggtgttg gcgagggcgc tccagcacga tctaaaaggt ccacttgcct
     1621 tcctggcggg gcgggaggat cccatcgagc tgcgcgtgca tgcgcagtac aatccggaag
     1681 ccatcacgca atacaatatc gtccccggtc taatgggggt ggtcattacg atgacgatgg
     1741 tgatgatcac aggcctggcg atcactcgag agcgcgagcg cggcacgatg gaaaacttgt
     1801 tgtcgatgcc gacccgaccg tttgaagtca tggttggcaa aatcatcccg tacatccttg
     1861 tcggctacgt acaactcggg ctcatcctgc tggcagcgcg cgttctgttc gaagtgccga
     1921 tgcatggcaa tctcggcctt ctgctgctgg tcgccttctt gttcattacg gcgaatcttg
     1981 ccatgggcat taccttttcc acgctggccg aaaaccagct tcaagccgta caattgtcat
     2041 ttttcttctt cttaccgtcc ttgctcttgt caggattcat gttcccgttc cgaggcatgc
     2101 ccgagtgggc gcaggctgtc ggtgagttgc tcccgttgac tcattttctc cgggtcgttc
     2161 ggggcatcct gttaaaaggg aacggcttgt acgacatcgt cctccagctc tggcaaatcg
     2221 ctctgtttgc ggtggtcgca ttagcggtgg gaataaagcg ctaccggcag acgctcgatt
     2281 agccccactt gactttggtt ctaactagaa ctatcttcgt tgatagcacc gcaacgttac
     2341 cggagaggcg gcgatgaaag cattctttcg aacagatgaa ggatggaccg gcttcattct
     2401 tcggacgacg ctggggctgg tgatgctgcc acacggcgcg cagaagctct tgggatggta
     2461 tggagggctt ggtttcagcg ggacgctggg cttatttacg gaaaagatgg gcataccgtt
     2521 gtcgatcgct tttctcgtta tcgtcgccga gtcattcgga agccttgcgc tgctcgccgg
     2581 gtttctcact aggttcgcgg cggctagtct agccgttatc atgctgggag cgattgtaat
     2641 ggtacacctg ccgcaggggt tctttatgaa ctggttcgga cagcagcagg gtgaaggatt
     2701 cgagtatcac cttctggtcc ttggcatcgc ggcagccctg ctggtgacgg gaggagaccg
     2761 ctggtctgtc gatcgcatcc ttgccgaaag gatcgcgcgt tgacaggaag ggaggacatc
     2821 atgacgacgg atgtggtgac gaaaaaggaa gtgctaggcg tctatcagcc aggatccgcg
     2881 catatggtcg gcgacgggtt tcccgtgcgc aatctctttc ccagcaatga gctggatcgg
     2941 gaggtcgatc ccttcctcat gctggactat gccggcccga gaaatttccc gccgaccgag
     3001 cacccgcgcg gcgtcggcga gcatccgcat agaggatttg aaaccgtgac gatcatgtat
     3061 gaaggcgcgg tcgcccatcg cgattcggca ggcaacgccg gcgtgattgg tccgggcgac
     3121 gtgcaatgga tgacggctgc ctcggggatc gtccatgagg aaatgcatga agcgcactgg
     3181 gcgaagcaag gcggcacgtt ccgagcgatt cagttgtggg tcaatcttcc gcaagctcac
     3241 aaaatgtcgg cgcctgccta ccaaacgctc gtgaatgatg atattccggc tgtcgatctt
     3301 gagggtgggt ctggccgact tcgtgtcatc gcgggcacgt ttcgaggcca caaaggaccg
     3361 gcgcgtacat ttacaccggt cgaactgtac gatcttcaat tacgggccgg agcgcggttc
     3421 gaattgacac tcccggaagg acacaatacc tccgtctttg tgcttgaagg ccgggtttcg
     3481 gtcaacggct ctgcagcggc cggtgaagcc gagttggttg tgagcaaggg gcagggatcg
     3541 ctggtaaggc ttgaggtcat tgacgacagc cggctcttga tcatgagcgg gaagccgatc
     3601 aacgagccaa tcgcccgcta tggccctttt gtgatgaaca ccagggctga gttggttcag
     3661 gcggtcgagg attatcagcg cgggaggatg ggacatttgt cccctctgga tgctggataa
     3721 gtccactgag cggagtttcg tgcggcgagc gtggacgggc gcgtcttggc gcgccggggc
     3781 tgggcgggtg aaatggacgg gcgcgtctcg gcgcgccggg gccggggtgg atgagaacct
     3841 gggacttaat catccgtgac aggaaattca gatggatcat cagaatacga gaataaccgt
     3901 cgaggtgtat tcggatgtgg tctgcccgtg gtgttacgtc ggcaagcggc ggttcgagcg
     3961 cgctcagtcc caactcccga cgactgcaac gcaggtcatc tggcggccgt tccaattaaa
     4021 cccaacgatg ccctgcgagg gcatggatcg gcgaacctat ctagaagcga agttcgggag
     4081 tcaagactct taccatcgtc tcgaagagca ggtcgcggcc gccggagctt cggagggaat
     4141 atctttcgca tttgagagaa tgcgtaggac gccgaatacc tttgtggccc atcgcctcat
     4201 ttggtatgcg gggcagcacg aacgacagaa tttggtcgtg gacgcgctgt tcaaagcata
     4261 ttttgaacag ggggcagaca tcggatcaca tgcagtcctg accgacctcg cggcatccgc
     4321 cggcctggac gccgcttcct ttctccagac ggaggaaggc gcggcggacg tcaaagccca
     4381 ggaggctgta ggacgacgtc tcggcatccg tgcagtcccc tgctttgtct tcaacaagag
     4441 cgacggcatc tcaggggcgc agccggtcga cgtgttcgtc ggcgccatca aaaacgctca
     4501 tgcgcgagac gccgaagatc aactgaccag aaagtaattc atgtctgtac aggtttacgg
     4561 ctgcaaccat gttgtgatcg aagtgactga tgcaaaaaaa gcggttcagt tctattcgga
     4621 cgtcttcggc ctcaaaatgc tgcgaggcgg cgaaggggcg gcctggtgca agttaggcga
     4681 acatcagttc atggccatct tcgaggtgga gaagctgcaa cccgaccgag tcaagcactt
     4741 cggtcttatg gtgcgggatc cggaacaaat caataccgtc cggcgtaagc tcacgagcaa
     4801 gtacaaactc aagttgcatc ccgatttccg ttgcgacttt cgggatccgt ggggaaaccg
     4861 cattcaagtt ggagacctct ccgatgaatc cttggtttgg ctccttccct atcaagaggt
     4921 tcaaaaggcc ggtctgattt tcagaagtgc atcgaaaagg cggcgccggg attgaaccat
     4981 gaccagacgc agccataccg ccacccaatt gcttcatctg tcgaaagctc tgctgcgttt
     5041 gcacaagacg ttgctcgatg gggagcgagc ggcgtacgaa cgtctgcacg gccccatctc
     5101 ttccaatgga gcgtttcttc agctcgtgct cggccatccg tcatttgcct ggctgcgcga
     5161 cctgtcgcgg tcgatggcac acttggacga actggccgaa gcgaaggagg cttctcctgc
     5221 cgaagcgctt cacgctcaaa cggcgtcgct ccgggcgctc ctgactccca tgaaggacgg
     5281 agaagaattc caccggcggt atcacgacgc cctcgaacgg gacccggacg ccgccctcgc
     5341 gcatgcggcg gtcatagaat tgctctctga tacccgctga aaaggagaag aggcatgaaa
     5401 gcccagtacc tcggccatgt ggttttttac gtcaaggatc tggaacaatc cctcgcattt
     5461 tatcgcgact tactgggctt caaagaagtc gggcggattt tcggcggagc cgccgccgca
     5521 ttgacctcag gacgcacgca tcatgagttg cttttgatcc aagtgggcga tgcgcctcct
     5581 cccccttcag gccgccgtcg tggtctctat catatcggta tcaaggtcgg ggacagtctc
     5641 gatgagctgc gctcggcgaa gcgcgaactc gatcaagccg gcatcgccat cgacgggatg
     5701 agcgaccaca ccgtcagcca gagcctgtac ctccgggacc ccgacggcaa cgaaatagag
     5761 ctctacgtgg atgccgatga ggcggtgtgg aaagcggatc ctgccgcggt gctatcgcca
     5821 atcaagtcac tgcggctcta gcctcttgag tcatcattgt tccttaggcc atactgagcg
     5881 gctatggacc aatctgcctc accgtgcgag accctgggag agcggtatca agcgctcctc
     5941 gaggttgccg aagccatttc cgcgcaccgc gacgtgcatg agctgtttcg ggatctcgcc
     6001 cgacgcctcc cccgcgtggt gcaggtgaat ttcgtcgcgc tctcgttgca tgatcctgcc
     6061 ggcaaccgga tgcgcctcca gactattcaa gcgaacgtcc cggctgatct cgtcggcggc
     6121 catgaagagc cgctcgagga aactccggct ggctcggtct ggcagtcgca acaaccgatc
     6181 ctcgtgcccg atcttgacgg agagctccgc tggcc
//
LOCUS       DASTQY010000326        10361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_681253, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000326 DASTQY010000000
VERSION     DASTQY010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10361
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681253"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFR82_10620"
     CDS             complement(<1..395)
                     /locus_tag="VFR82_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502096.1"
                     /translation="MNRRIMMSLVLCGLCLLAAAAGILESGAADVFLEATRPDFQKIP
                     LGVVSIENNGGPDWLGGRLEEVLKKDVKRSLVFELLDLPSLGIKVRDVGNGHGNGSKA
                     VFKQAAENGVSVLVWGKAGLKKGEKEDDV"
     gene            complement(392..1387)
                     /locus_tag="VFR82_10625"
     CDS             complement(392..1387)
                     /locus_tag="VFR82_10625"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU4502097.1"
                     /translation="MVARAQVHSAFWFDHDEQDRFLARLGRTLTISLVLHLVALLIAM
                     GIRLTPHGERPLASIQVNLVSLPTPVKPVEQSRPVESVKRVEPKPVPVSAPPVRMPAP
                     AAPSVSTPARTSVAKDILKDLELPPDAPKFGDLTPVKSVAQPRPQVQAKVHVPDLPRI
                     PDLMPDPVTKTPQRTSISEELDRELEEELKKVKEFKPAAKLDIPKEAPVKAVPHQEAA
                     IPQAKAPQTQLKTSGSSGTNPFWGRVEAIIRGQWEPPPIDVTGQTYSAVVRFRFYRNG
                     TVKDVVIQQTSGNSYFDMSGQRAILKPRQFPAFPAEMTDAYQDVEMVFRVGEIAG"
     gene            complement(1416..1868)
                     /locus_tag="VFR82_10630"
     CDS             complement(1416..1868)
                     /locus_tag="VFR82_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU4502098.1"
                     /translation="MMFESRQRRFMAEINVIPLVDVVLVLLVIFMVTAPMLYRGMDIN
                     LPKSASNTIKPEMRTVLTIEKDQRLYLDKDPVGVAQLEQKLKVLKHEHADVSLYLRAD
                     REVPYGIVVQVMDGVKKAGIEKIGMVTEPVGPEHIGGASIPRGPSRKS"
     gene            complement(1865..2557)
                     /locus_tag="VFR82_10635"
     CDS             complement(1865..2557)
                     /locus_tag="VFR82_10635"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEU4502099.1"
                     /translation="MFQIGLTGLLGSLGAVSKVVLLLLFVFSVVCWAIIFLKWRAFSA
                     ADRMDHQFMALLAKAKDLDDLCRQVRRMETSPAAILFEGVMDRVAGLRGQDGAGLADR
                     QLVERTAAHLSHSQLARLESYLPFLATTGNITPFIGLLGTVMGIIDAFREIGAQGTAS
                     IAAVAPGVAEALIATAAGLFAAIPAVIAYNYFLSRIRRTAFRLDTVTIELMTSLSIPT
                     IKAKPIPVGAQR"
     gene            complement(2756..2834)
                     /locus_tag="VFR82_10640"
     tRNA            complement(2756..2834)
                     /locus_tag="VFR82_10640"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2796..2798),aa:Asp,seq:gtc)
     gene            complement(2948..3023)
                     /locus_tag="VFR82_10645"
     tRNA            complement(2948..3023)
                     /locus_tag="VFR82_10645"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2988..2990),aa:Val,seq:tac)
     gene            complement(3108..4061)
                     /gene="xerD"
                     /locus_tag="VFR82_10650"
     CDS             complement(3108..4061)
                     /gene="xerD"
                     /locus_tag="VFR82_10650"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU4502100.1"
                     /translation="MAIIAEMETDALVERYLNHLRVEGGLARNTIEAYRRDLAKFQRY
                     LSERKIGLRTLLPPETVRSFMGSLHAARLSPVSSARCVSAVRGWLRFLRQERIIDDQT
                     AFDVAPLRRGIRLPKTLSPREVATLLDRPSQLTLEDQRDRVMLELLYATGLRVSELIS
                     VETLQVNFEVGYLRVTGKGSKQRIVPMGEPARRLLLEYRETIRPRFLRERASRYLFVS
                     RRGTPLSRQAFWKSLRARAQRAGLTKAVSPHMLRHSFATHLLGGGADLRSVQTMLGHA
                     DIATTQIYTHVERERLKEVHANFFPRQRRRKEGRAVTEARK"
     gene            4150..6147
                     /locus_tag="VFR82_10655"
     CDS             4150..6147
                     /locus_tag="VFR82_10655"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEU4502101.1"
                     /translation="MRTSHDRHFVRRALRLPDLHRIQLPVAACVALTLALVTFAMPER
                     HPVAAAETAKRAPASHPSLNQAKRLIDAGQAEEAITILRRFLGTNPKPEFLDDTYLLL
                     GAALYRGQQYPEALKYLQQLQTEFPSSEVIDRGKLMLARTHAAMGNPDLALPILTGLR
                     ALSQDDVTKREAVHLSGELYFQKKEPARAIQAWLDEIALSSDDQGEEIRTNITEVITE
                     SFDKKALERIREAYPKQFPGDLAALRLIDLYMGRGEDHQASRQIQQFLTHFPTHPQRN
                     RLSEMLEALQTKLKSSQYVMAAVLPLSGKLSSFANDVLSGVQLAVESGGERGGGPSIG
                     LLVKDLDASHASFLDDFSHLLFNDRPVAVIGPLLSKNLPVMAELAERSHTPLLTPTAM
                     LPNVRRLGSFTFSTAVTYQLQARKIAAYATGDLGFRRFCILHPDTAYGREMARLFAHE
                     ARQRDGEIIAIESYKEGDSDVGAQLKRMKAEDLKKYGLAVPVDPLKVGGKLTKLDKKV
                     LYTPGFDAVFIPGRAADVGLIAAQLNFHDMKVPFLGSNGWNAPDFARTADQSIDGAVF
                     VDGFFAESPNPSIQDFVDRYKKRFQSAPTLFAMQGYDAAKFVVDAIKKGATSGDAIRD
                     YLTSQQDLPALAGPAAFAQDGTLNRPLFLIQVKRGRFTQVD"
     gene            6252..6995
                     /locus_tag="VFR82_10660"
     CDS             6252..6995
                     /locus_tag="VFR82_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU4502102.1"
                     /translation="MNDVTQILHAVSYKALPLLFAIVLHEYAHGWVANKCGDSTAKLQ
                     GRLTMNPLAHIDPFGTIIVPLICLMLPGSFLLGWAKPVPVDPRNMRQPRRDMAIVAAA
                     GPGMNLTLAVASAGLLALLLSIDPLLMTSDSRIDTLEAAPSPAFRFLLPVAVMALYSV
                     LVNVFLAIFNLMPIPPLDGGRILLSLLPPRPALVLARLEPYGMMILVGLLVFDRELRV
                     IHTFTSTFASGLSGTILSTAIGFSAGTSP"
     gene            6992..7984
                     /gene="trpS"
                     /locus_tag="VFR82_10665"
     CDS             6992..7984
                     /gene="trpS"
                     /locus_tag="VFR82_10665"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU4502103.1"
                     /translation="MTSRRKRVLSGMQPSGLMHLGNYLGALQNWKHLQTEYECYFFVA
                     DWHALSTNYADTSRIKEFVRELLIDWLAAGIDPNRSIVFIQSRVPEHAILHLLFSMII
                     PVSWLERNPTYKEKQEEIKEKDLTTYGFLGYPVLQAADILLYKPDFVPVGKDQLPHLE
                     LTREVGRRFNDLYKQQVFPEPREHLTKFPKVPGTDGRKMSKSYGNTINLSDTEPVVRQ
                     KLKTMVTDPARVRRMDPGNPDLCPVFDFHKMFSALPIIEQVNRECRTAEIGCIDCKKL
                     VADRVVETLAPIWDARAALLKNPSGIEDLVQAGSQRAATVARATLHEVTEAMKI"
     gene            7981..8406
                     /locus_tag="VFR82_10670"
     CDS             7981..8406
                     /locus_tag="VFR82_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502104.1"
                     /translation="MRDDRPNESPVLAQRGLRRPSLDARSWRPIRAGHEEVRMESHGG
                     KKLVIAVALWQLTGVHAWAVDPPCDAFPPAKQLRCVEVWKDLYKEDGPAIAQFGLDQL
                     KRREEGKITAQQHLAQNMDFIKQATERRLARLNERMAKE"
     gene            complement(8477..8983)
                     /locus_tag="VFR82_10675"
     CDS             complement(8477..8983)
                     /locus_tag="VFR82_10675"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEU4502105.1"
                     /translation="MRTSARWLAIFVFFFAWGPPVVHAEAGSGSQSDQVTIADVKVRI
                     SDHGPVVLLQAEGKTIPIFVDLTVAVSIQGALNGEQLHRPLSHDLMHTILDTFGAKVT
                     QSVITLKGGTYYGALSVSFRGDVKVFDSRSSDSIALAIHFKAPIIVGRDLLNSAGVVP
                     EEPKPQDL"
     gene            complement(9002..9640)
                     /locus_tag="VFR82_10680"
     CDS             complement(9002..9640)
                     /locus_tag="VFR82_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4502106.1"
                     /translation="MAELIRKTFAVPPLGCNCSILGDPVTKLAVVVDPGGAAERILRE
                     VQDLGLTVTKILHTHAHFDHFLASGEIKKATAATLCLHKDDRELWDMLEIQCQAFGVP
                     YIPVPPPDYWLADEETIQIGQVSAIALHTPGHTPGSMSFHLPTARVVLTGDTLFRGSI
                     GRTDLWGGDFDAIERSILERLYTLEDATAVVTGHGPETEIGWEKDRNQFVRA"
     gene            complement(9757..>10361)
                     /locus_tag="VFR82_10685"
     CDS             complement(9757..>10361)
                     /locus_tag="VFR82_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502107.1"
                     /translation="GSVSPAPAETGPDTSAAVPAEDYPLYDLIVDEKFLTPETKLVVL
                     ERVTLTRLHPEQQGPLRPETFHQYEIFDGRLPGELVRDFLVKNQAPSKLDGRFGFGVR
                     YRFVSGDGVEQQETSLPSILVSESPRFMQEEPLDEPPAILDRLAFSRVAYTLGREQAL
                     IYVEYNRPTGAGAGFAVWLHRSGAHWGIFDSELVWAGRPQ"
BASE COUNT         2241 a         3107 c         2890 g         2123 t
ORIGIN      
        1 ttgacgtcgt cttctttctc gcctttcttg agacctgcct tgccccagac cagaacggac
       61 acgccgttct cagccgcctg cttgaacacg gcctttgacc cattgccatg cccatttccg
      121 acatcacgca ccttgatacc caagctggga aggtccagca gttcgaacac cagcgagcgc
      181 ttgacgtcct ttttcagcac ttcctcgagg cgtccgccga gccagtcagg cccgccattg
      241 ttttcgatgc tgaccacacc gagcggaatc ttctgaaagt ccggcctcgt cgcctcgaga
      301 aagacgtcgg ccgcaccaga ctccagaatg ccggcggctg cagccaacag gcaaagcccg
      361 cacaagacaa ggctcatcat gattcgtcga ttcatccggc gatctccccg actcgaaaca
      421 ccatttcaac gtcctgatag gcgtcggtca tctccgcggg aaacgcagga aattgacgcg
      481 gcttcaagat ggctcgctgt cccgacatgt cgaaatagct gttcccagac gtttgttgga
      541 ttaccacatc cttgacggtc ccattccgat agaacctgaa tcggacaact gcactgtacg
      601 tttgcccggt cacatcgatg ggaggcggct cccattgacc cctgatgatc gcctccaccc
      661 gcccccagaa cggattcgtg ccggatgacc ccgacgtttt caattgcgtt tggggtgcct
      721 tcgcctgcgg gattgcggct tcctggtgag gaacggcttt gacgggtgct tctttcggga
      781 tgtccagttt ggcggccggt ttgaattcct tgaccttctt gagctcctcc tcgagttcgc
      841 ggtcgagctc ttcgctgatc gacgttcgtt gcggagtctt cgtcacggga tccggcatga
      901 gatccggaat acgaggcagg tcgggaacat ggactttcgc ctgtacctgc ggacggggct
      961 gtgccaccga ttttaccggc gtcaaatctc cgaatttcgg cgcatccggc ggcaactcca
     1021 gatccttcag aatatctttg gcgacactgg tacgcgccgg cgtcgaaaca gagggagccg
     1081 cgggggcagg cattctgacg ggtggagcgg aaaccggaac cggcttgggt tccactcgtt
     1141 tgacagattc gaccggcctg ctctgctcca caggtttcac cggcgtgggt aaactgacca
     1201 ggttcacttg gattgaagcc aaaggccgct caccgtgagg agtcagccga attcccattg
     1261 cgatcagcag agcgaccaga tgcaaaacca gggagatggt gagcgttcga ccgaggcgag
     1321 caagaaacct gtcctgctcg tcatgatcga accagaaagc cgaatgcacc tgtgcgcgag
     1381 cgaccatccc aacatccctc tcagcagcga cgtgtttagc tcttacgaga gggtccgcgc
     1441 ggaatcgatg cccccccgat gtgctccggt cccaccggtt cggtcaccat tccgatcttc
     1501 tcgattccgg ctttcttcac cccatccatg acctggacga cgatgccgta tggcacctcg
     1561 cgatcggccc ggaggtagag cgacacatcc gcatgctcgt gtttcaagac tttcagtttt
     1621 tgctcaagct gggcgacgcc gaccggatcc ttgtccaagt atagccgttg atctttttcg
     1681 atggtcaaca ccgttcgcat ttccggcttg atcgtgttgc tcgcagattt cggcagatta
     1741 atgtccatac cgcggtacaa catcggcgcg gtgaccataa aaataacgag cagcaccagc
     1801 acgacgtcga ccagcgggat cacgttaatc tccgccatga agcggcgctg gcgcgactcg
     1861 aacatcatcg ttgcgctccg acgggaatcg gcttggcctt gatggttggg atcgagagtg
     1921 acgtcatcaa ctcgatggtc acggtgtcca atcgaaatgc ggtccggcgg atcctggaga
     1981 gaaaataatt gtatgcgatg accgccggga tcgccgcaaa caagccggca gccgtcgcaa
     2041 tcaaggcctc agcgacgccg ggagccacag ccgcaatgct ggccgtgcct tgagcaccga
     2101 tttcacggaa cgcatcgatg attcccatca ccgttcccaa caggccgata aaaggcgtga
     2161 tgttgcctgt ggtcgccagg aatgggaggt aggattccag tctcgccaac tggctgtggg
     2221 aaagatgggc ggcggttcgt tccacgagtt gacgatcggc gagacctgct ccgtcctgtc
     2281 cacgcaaccc cgcaacgcga tccattacgc cttcaaacaa gatggccgcg gggcttgttt
     2341 ccatgcgtcg cacctggcgg cacagatcgt ccagatcctt cgccttcgcc agaagagcca
     2401 tgaactgatg atccatgcgg tcagctgccg aaaacgcacg ccacttgagg aagatgatgg
     2461 cccagcacac gacggagaac acaaagagca gcagcagcac aactttcgaa actgcgccca
     2521 gtgagcccag aaggccggtc aagccgattt gaaacatggg tcgatacctt tcgtggtctc
     2581 gccgaagagt ccgtctaccc ttgggagtgt aacaaatgcg tgaggaaatg cggcgctggc
     2641 gcccgtgacc actggcctaa caggggcatc gctcaagacg gcctgactcc attgaaaccg
     2701 ctgaaaccgc ctgcggaggt gtatcccaat ggccatatat gcgggacgaa aaagatggcg
     2761 gggccgacgg gatttgaacc cgcgacctcc agattgacaa tctggcgtcc taaaccaggc
     2821 tgaacgacgg cccctcgtga ctctacctat tgcggatgat ttgattgatc agacctatat
     2881 atagcggatg gtcccgtctg tctagaatct ttgtccttac tgcatgaacg aaaccgcccc
     2941 gccgtggtgg taggcggaac agggatcgaa cctgtgacct ctgccttgta agggcagcgc
     3001 tcttccaact gagctatccg cccgatttcc ttaataagct ggcggatgct atcaagtccg
     3061 ttcattcttg tcaaccgaat ctcgcactgt tcttgcacat tcttcggcta cttccgagct
     3121 tctgtgacgg ccctaccctc cttccggcgt ctctgccgcg ggaagaaatt cgcatgtact
     3181 tccttgagcc gctcgcgttc gacgtgcgta tagatctgcg ttgtcgcaat gtcggcatgt
     3241 ccgagcatcg tttgcacaga acgaagatcc gcacctcccc ctaacaaatg cgtggcgaac
     3301 gaatgccgca acatgtgcgg ggacacggct ttcgtcaacc cggcccgttg agcccgtgct
     3361 cgcaaggatt tccaaaatgc ctggcgcgag agaggcgtcc ctcggcgcga gacaaacaaa
     3421 tatcgcgacg ccctctctct caagaagcgc ggcctgatcg tctcccgata ctccagcagt
     3481 aaccgccggg cgggctctcc catcggcacg atccgttgtt ttgaaccctt tcccgtcaca
     3541 cgcagatagc ccacctcgaa attcacctgc aacgtctcga cggagatcaa ctcggagaca
     3601 cgaagtcccg tcgcgtaaag caattccagc atcacgcggt cgcgctgatc ctccagcgtc
     3661 aattgagacg gcctgtccag caaggtcgca acctcgcgcg gggacagcgt cttgggcaga
     3721 cggattccgc gccgtagcgg agccacatcg aacgccgttt ggtcatcgat aatccgttct
     3781 tgacgcagga agcgaagcca tccgcgaacg gcggatacac aacgggcaga cgagacgggc
     3841 gacagccgcg cggcatgcag cgaacccatg aagctccgca cggtttcagg cgggagcagc
     3901 gttcgcaagc cgatcttccg ctcgctgaga tagcgctgaa atttcgcgag atcgcgccgg
     3961 taggcctcaa tggtattacg agcgaggccg ccttcgacac gaagatgatt gagataccgt
     4021 tcgaccagcg cgtcggtttc catttcggcg atgatagcca atcgttctta tgaacacaac
     4081 gcgacggcgg gtgcgtgaaa ccttgacacc ctccggggat tccgatacta gtcactcttg
     4141 acgatcttca tgcgtaccag ccacgatcgc cacttcgtcc gtcgcgccct acggctgccc
     4201 gacctccatc gtatacagct tccggtcgcc gcgtgtgtcg cgctcacgct ggcgcttgtt
     4261 acgtttgcca tgccggaacg ccatcccgtc gctgccgcag agacggccaa gcgtgcgccc
     4321 gccagccatc cctccctgaa tcaggccaaa cggttgatcg atgccggaca ggcagaagag
     4381 gccatcacga ttcttcggcg atttctcgga accaacccga agccggagtt ccttgacgac
     4441 acgtacctac tccttggggc ggccttgtac cgaggacagc aatatccgga ggcgctcaaa
     4501 tatctgcagc aacttcaaac agagtttccc tcctcggaag tgatcgaccg cggcaagttg
     4561 atgctggcac gtacccatgc cgcaatgggg aaccccgatc tggcgttgcc gattctcacc
     4621 gggctccgcg ccctctcaca ggatgatgtg acgaagcgcg aagccgtgca tttgagcgga
     4681 gagctgtact ttcagaaaaa ggagccggct cgggcgattc aggcgtggct cgacgagatc
     4741 gcgctgagct cggacgacca aggagaagaa atccggacca atattaccga ggtgatcacc
     4801 gaatcctttg acaaaaaagc gctcgagcgt atccgtgaag cgtatccgaa acagtttccg
     4861 ggggatctgg ccgcgcttcg gctgatcgac ctatacatgg gacgtggcga ggatcaccaa
     4921 gcgtcccgac aaattcagca atttcttacc catttcccta ctcatcccca gagaaacagg
     4981 ctctcggaga tgctggaggc gctgcaaacc aagctaaaat ccagccagta cgtaatggcg
     5041 gccgtactgc cgctgtcagg aaagctctcg tcgttcgcga atgatgtgct gagcggcgtc
     5101 caactcgccg tggaatcagg cggcgagcgt ggcggcggac cctccatcgg cttgctggtc
     5161 aaagatctcg atgcttcgca cgcctctttt ctcgacgatt tttcccatct actgtttaat
     5221 gaccgtccgg tcgcggtcat cggcccgctc ttgtccaaga accttccggt gatggccgaa
     5281 ctggccgaac gctcgcacac cccgctcctg acgcccaccg ctatgctgcc gaacgtccga
     5341 cggctgggca gctttacgtt cagcacggcg gtgacctacc aactgcaggc acggaaaatt
     5401 gccgcgtatg ccactggcga cctcggtttc cgccgatttt gcatccttca ccccgacacg
     5461 gcgtacggac gtgagatggc ccgcttgttt gcccacgaag cgcggcagcg cgatggagag
     5521 atcatcgcga tcgagtccta taaagaaggc gactccgatg taggtgcgca gttgaaacgg
     5581 atgaaagcgg aggacctcaa gaaatacggg ctcgccgtac cggtagaccc cctgaaggtg
     5641 ggcgggaaac tcacgaagct cgacaagaaa gtgctgtaca ctcccggctt cgacgccgtg
     5701 ttcattcctg ggcgagccgc ggacgtcggc ttgattgccg cgcaattaaa ctttcacgat
     5761 atgaaggtcc cgtttcttgg gagcaacggc tggaacgccc ccgattttgc aaggacagcg
     5821 gatcaatcca tcgacggtgc ggtgttcgtc gacgggtttt tcgccgaaag ccccaacccc
     5881 agcattcaag attttgtcga ccgctataag aagcgcttcc aaagtgctcc aacactcttc
     5941 gccatgcagg gatatgatgc ggctaaattc gtggtcgacg cgatcaagaa aggcgcgact
     6001 tctggcgacg caatacgcga ttatttgacg agccagcaag acctgcccgc gctggccgga
     6061 ccggccgcct tcgcgcaaga cggtacgctc aaccggcctc tctttttgat acaagtgaaa
     6121 cgtggacgtt tcacacaggt ggactaagca ggagtgaacg ataggccggc tgtatcggtc
     6181 gccttgagag agggctaccg aacaggtacc ctcgtcatga tcaatcctga agtcactcgc
     6241 gatctatttc catgaacgat gtcacgcaga tcctccatgc cgtctcgtat aaggcccttc
     6301 ccttgctctt cgcgatcgtc cttcatgaat acgcccatgg atgggtcgcc aataaatgcg
     6361 gcgattcaac agccaaactt caggggcgtc tcacgatgaa tccattggct catatcgatc
     6421 cattcggcac catcatcgtt ccactgattt gcctgatgct gccgggcagc tttcttcttg
     6481 ggtgggccaa gcccgttccg gtcgacccca gaaacatgag acagccgcga cgcgacatgg
     6541 ccatcgtcgc cgccgcggga cctggcatga atctcacgct ggcagtagcc agtgcgggac
     6601 tgctggcgct gctcttgtcg atcgatccgt tgctgatgac gagcgattcg aggatcgaca
     6661 cgctggaagc cgccccctcg cccgctttcc gattcttgct cccggtcgcc gtgatggctt
     6721 tgtattcggt cttggtgaat gtgtttctcg ccattttcaa tctcatgccc attccgccac
     6781 tggatggcgg gcgaatctta ttgagtctgc tgcctccgcg ccccgccctg gtgctcgcgc
     6841 gattggagcc ctatgggatg atgatcctgg ttggcctcct cgtctttgac cgggaactcc
     6901 gcgtcatcca cacctttacc agtacattcg cgtcggggct ttcaggtaca attctgtcga
     6961 cggcgatcgg atttagtgca ggaacctccc catgacaagc cgacggaaac gagtgctgag
     7021 cggtatgcag cccagcggcc tcatgcatct tggcaattac ctcggtgcac tccagaactg
     7081 gaagcacctt cagactgaat acgagtgtta ttttttcgtc gcagattggc atgcgctctc
     7141 cacgaattac gccgacacct cccggatcaa agaatttgtg cgggagctcc tgatcgactg
     7201 gttggcagcc ggaatcgatc ccaaccggtc gatcgtcttc attcagtcgc gtgtgccgga
     7261 acacgccatt ctgcacctgt tgttttccat gatcatcccg gtgtcatggc ttgagcgcaa
     7321 tccgacgtac aaggagaaac aggaggaaat caaggaaaag gatctgacga cttacggctt
     7381 tctcggctat cccgtgctgc aagcggccga tattctcttg tacaaacccg atttcgttcc
     7441 ggtgggaaaa gaccagctcc cacacttgga actgacgcgt gaagtggggc gacgattcaa
     7501 cgatctctac aaacagcagg tatttcccga gccgagagaa catctgacca agtttcccaa
     7561 agtgccgggc actgacggca ggaaaatgag caagagctac ggcaacacca tcaacctgtc
     7621 cgacactgaa ccggtcgttc ggcagaaatt gaagacgatg gtgaccgacc ccgctcgcgt
     7681 caggcggatg gaccctggaa atccagatct ctgcccggtt ttcgattttc acaaaatgtt
     7741 ctcagcgctg ccgatcatcg agcaggtcaa tcgagaatgc cgcacagcgg aaatcggctg
     7801 tattgattgc aagaaactgg ttgcggatcg agtggtggaa accttggcgc ctatatggga
     7861 cgcccgggcg gctctcctca agaatccttc cgggatcgaa gacctcgtgc aggcaggcag
     7921 ccaaagggcc gccaccgtcg caagggccac gttgcacgag gttacagagg cgatgaagat
     7981 atgagagatg accggcccaa tgagtctccc gtgctcgcgc aacgcgggct cagaagaccc
     8041 tcgttggacg cgcgcagttg gaggcccatt cgggccggtc atgaagaagt gaggatggaa
     8101 tcacatggag ggaagaagct ggtcattgca gttgcacttt ggcaactcac cggagtccac
     8161 gcgtgggcgg tggatcctcc ctgcgatgcc tttccgcctg caaagcagct ccgctgcgtg
     8221 gaggtctgga aggacctgta caaagaggac ggcccagcga tcgcccaatt cggacttgat
     8281 cagctgaagc gccgtgaaga aggcaagatt actgcacagc agcacctcgc ccaaaatatg
     8341 gacttcatca aacaagcgac ggaaagacgt ctcgcgcgat tgaacgagcg catggcgaag
     8401 gagtaagcgc ggtcgagtta tgcttcgcgt ctcgagacga cgaagcttgt cggcgacgga
     8461 caataacctc gggtgttcat agatcctgcg gcttcggttc ctctggaacg acccccgccg
     8521 agttcaagag atcccgcccc acaataatcg gcgccttgaa gtgaatggcc agcgcgatgg
     8581 aatccgaaga gcggctgtcg aacaccttga cgtcgccgcg gaacgaaacg ctcagcgctc
     8641 cgtaataggt gccgcccttc aaggtaatca ccgattgcgt caccttcgca ccgaacgtgt
     8701 caagaatggt atgcatgaga tcgtgagaca agggccgatg caactgctcc ccatttaatg
     8761 cgccctggat agagaccgcg acggtgagat cgacgaagat gggaatggtt ttgccttcgg
     8821 cctgcagaag caccaccggc ccatggtcgg aaatgcgcac cttcacgtcg gcgattgtga
     8881 cctgatccga ttgcgagccc gagcccgcct ctgcgtgcac gacgggcggc ccccatgcaa
     8941 aaaagaacac gaatatcgcc agccatcgag cacttgtcct catagcctcc tcctcatccg
     9001 ttcaggcgcg aacgaattgg ttcctgtctt tctcccatcc gatctccgtt tcgggcccat
     9061 gcccggtcac gacagccgtc gcatcttcga gcgtgtacaa ccgttcaaga atcgagcgct
     9121 cgatcgcatc aaaatcgccg ccccacagat cggtccgtcc gatgctgcca cgaaagaggg
     9181 tatctcccgt cagcacgaca cgcgcggtcg ggagatgaaa gctcatggat cctggcgtgt
     9241 gccctggcgt gtgaagggca atagcgctca cctgcccgat ttggattgtt tcttcgtctg
     9301 ccagccaata gtctggcggt ggaaccggaa tatacggaac tccaaacgcc tggcattgga
     9361 tttcaagcat gtcccacaac tcgcgatcgt ctttgtgcaa acacaatgtc gcagcggtag
     9421 ccttctttat ttcgccggat gccaaaaagt ggtcaaaatg tgcatgggta tgcaggatct
     9481 tcgtcaccgt cagtccgagg tcttgcacct cacgaagaat ccgctcggcc gcgccacccg
     9541 gatccacgac aacggccagt ttggtcacgg ggtcgccaag gatcgagcaa ttgcagccga
     9601 gcgggggtac agcaaacgtc ttgcggatga gttctgccat cgcaaaaggc tcttcaagta
     9661 tgctaacgcg cagaccgttc agctgcaact gaggggaagg aggtccgtga cggaacgatg
     9721 tggctggcgg cggcccagtc aggggaaggc agcccgttac tgaggccgac ctgcccagac
     9781 caattcgctg tcgaagatgc cccagtgcgc tccggaccgg tggagccaca ccgcaaaacc
     9841 ggctccagct ccagtcggtc ggttgtattc gacatagatc agggcctgtt cgcggccgag
     9901 cgtatacgcg acgcgtgaga aggccagccg gtcgaggata gccggcggtt cgtcaagcgg
     9961 ctcttcttgc atgaagcgag gactctccga cacaaggatg gacgggagag acgtttcctg
    10021 ttgctcgact ccgtccccag aaacgaaccg gtatcgcacc ccaaacccga aacgcccatc
    10081 gagcttcgaa ggcgcttggt ttttcacgag gaagtcacgt acgagctcgc ccggcagacg
    10141 accgtcgaaa atctcatact gatgaaacgt ttccggcctc agagggccct gttgctccgg
    10201 gtgcaatcgt gtcagtgtta cccgttcgag cacgacaagt tttgtttccg gcgtgaggaa
    10261 cttttcgtcg acgatgagat cgtacaaagg ataatcctcg gcgggtactg cggcagaagt
    10321 gtcagggcct gtctcagcag gcgcgggaga tacagagccc a
//
LOCUS       DASTQY010000327         4548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_39016, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000327 DASTQY010000000
VERSION     DASTQY010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39016"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFR82_10690"
     CDS             <1..479
                     /locus_tag="VFR82_10690"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEU4502108.1"
                     /translation="LWTWALPMSREINAAVASHPFDFQLWSSFKQKDLEHEGRAILRY
                     EHELVNKLSGQAVLVEFQGETVPSVQSAILTSQIGERLSAEAPFCIIWHDRDGRRYYS
                     MRSREDGTDVGAIAASFGGGGHTHAAGFSIPLGPDGSLPDNPALPRSIVDRRRNSA"
     gene            488..1195
                     /locus_tag="VFR82_10695"
     CDS             488..1195
                     /locus_tag="VFR82_10695"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU4502109.1"
                     /translation="MIPLQDDNPSERPPVVTIGFIIICVLVFFYQASLLPQPGEIFVF
                     QYGAIPAILFGQADLPPAMAVGIPAYATVITSMFLHGGWMHLIGNMLYLWIFGDNVED
                     VMGHVRFVVFYLTCGILAALSHAITDPTSTIPMVGASGAISGVLGAYLLLFPHAHVLV
                     FLPGIGMTRVAAGIVLGMWFVMQLLSGGMSIGGPGGGVAFFAHIGGFVAGMGLIGFFK
                     RPEVRFFSPAKTRRRDL"
     gene            complement(1273..3258)
                     /locus_tag="VFR82_10700"
     CDS             complement(1273..3258)
                     /locus_tag="VFR82_10700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4502110.1"
                     /translation="MTVPFCEVLYVQNHQTALELSKIKFLLDEGKGTGLARVLPGTAG
                     GGTMLGTLAFSQDSISSHDTDDQGAVSRPRYVILQLLVAIMLGYQLLFGPELIVSRTA
                     SEFIVGGLVVVIGCLLAVPFSVLQAAWFSGTLIAVDTALVTGTMYLAGQARSELYLSY
                     FILMLIAASLRRLSHVIAFSLLLCAGYGFVLYQGSVQTGVLSVGQLLGVPVLLVMAIF
                     YGMALETIGAERQQKMQLLHNIDALKKTEQALEASRDQLETRIKGLKEDLSKANALVQ
                     QEKSERQGLERQLVEAQKMEAIGRMTTGLANEFSHLFSVIGKQTGVVLSHLLPEDPLY
                     GAVDDIFRTGERAAALTAQLATLDIEHRQFRHVLSMRTLMEDLSETIRDLLPNRIECQ
                     ISAEGSPLDVEVDREGIEQMLLNLALNARDAMTGGGRLTISISSCEGGAAVKALASVN
                     RRASAVRIQVCDTGSGMTRDTQSRMFEPFFSTKETNVGLGLTSVYGVVKQHGGQVEVA
                     SQPGHGTTVRVYLPAVAAGMVPNEPAESSVAAKGQETILLVEPHELERKLAVSTLKRH
                     RYQVLEASSPVEAIMLAHQYSGAVHVTVSGLIMPEISGRELARRLLKQHPTMKALFVS
                     GYDDEAIISHRINRRFLLRRPYRQRGLVEKVRELIDA"
     gene            complement(3297..>4548)
                     /locus_tag="VFR82_10705"
     CDS             complement(3297..>4548)
                     /locus_tag="VFR82_10705"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU4502111.1"
                     /translation="DQCAAVSRGQRFRIGPQVIDASTPSPVNSQLVEPGSVLGVLGGG
                     QLGAMFAQAATRLDYRVAVWDPDPDAPAHRLAAHSFPSLFTDPQAFTQFADLVSVVTY
                     EWENVPAVLCERLERTTPVRPASSVLRLIQNRLVQKTFLAGQGFPVPRFAALTEPDKL
                     PEILEQLGYPAVCKTATAGYDGKGQWKIDEPSSVSSVKESLMASSHSTMEWIVESFVP
                     FERELSVLVARGIDGQTIVYPLVENVHEAGILRMTIVPAEVPFNVVERSADLARAVVA
                     RMEGIGLFCLELFLLPDQRVLINEVAPRPHNSGHYTLDACNVSQFEQQVRAVTGLPPQ
                     EVVLTNPAVMVNLIGEDAETIKRDVECRRLLGLPGAYVHDYGKRAIRPRRKMGHVTFL
                     AHDVQTARDRGSRLRQRLSLASRT"
BASE COUNT          972 a         1355 c         1253 g          968 t
ORIGIN      
        1 atttgtggac gtgggcccta ccgatgagcc gcgagatcaa cgcagcggtg gcctcgcatc
       61 cgtttgactt ccaactctgg agcagtttca aacaaaagga cttggagcat gaaggccgtg
      121 ccattttgcg gtacgaacac gaactggtga acaagctttc aggacaagcg gtgctcgtcg
      181 aattccaggg ggaaaccgtc ccttccgtgc aaagtgcgat cttgaccagc cagatcggcg
      241 agcgactctc cgccgaggcg ccattttgca tcatttggca tgatcgcgat ggacggcggt
      301 attacagcat gcggtctcgc gaagacggaa ccgacgtcgg tgcaatcgcg gcatcattcg
      361 gcggcggcgg ccatacccat gctgcgggct tctccattcc gttgggtccc gatggctcgc
      421 ttccggacaa tccggcgctc ccacggtcca tcgtcgaccg ccgtcgcaat tccgcttagg
      481 cggatccatg atcccgcttc aggacgacaa tccatccgaa cgccctccag tagtcacgat
      541 cggcttcatc atcatctgcg tcttagtctt tttctatcag gccagcctgc ttccgcaacc
      601 gggagagatc ttcgtctttc agtacggcgc gatccctgcc atattgttcg gccaagcgga
      661 tctcccgccc gccatggccg tcggcattcc cgcttatgcc actgtgatca cgagcatgtt
      721 cttgcatggt ggatggatgc atctgatcgg gaacatgctg tacttgtgga tctttggtga
      781 caatgtcgaa gacgtcatgg gacacgtccg gtttgtcgtg ttttacctca cgtgcggcat
      841 cttggcagcg ttgagtcacg ccatcacgga tccgacgtcc acgattccca tggtcggagc
      901 cagcggcgcc atttccggcg ttctcggcgc ttacctcctt ctctttcctc atgcccacgt
      961 actggtgttt ctacccggaa tcgggatgac acgagtggcc gccggtattg tcctgggcat
     1021 gtggttcgtc atgcagctgc taagcggcgg catgagtatc gggggaccgg gcggaggagt
     1081 cgcgttcttt gcgcacatcg gcggctttgt ggcgggaatg ggcctgatcg gcttcttcaa
     1141 acgtccggaa gtgcggttct tctcgccggc caagacccgc cgacgggatc tctagaacct
     1201 cggtaagaac gtctgatcaa tcggcccaga gcgaaaaata agcggctctg ccactacaat
     1261 cgcagtgacc tgctaggcat cgatcagctc acgcaccttt tccaccaacc ctcgttgccg
     1321 atacggccgc ctgagcagaa accgtcggtt gatgcggtga gaaatgatgg cttcatcgtc
     1381 gtatcccgat acgaacaagg ctttcatcgt cggatgctgc tttaacagcc gtctcgccaa
     1441 ctcacggccg ctgatttccg gcatgatcag cccgctgacg gtgacatgta cggctcccga
     1501 atattgatgc gcaagcatga ttgcttcgac gggcgaactc gcttcgagca cttgatatcg
     1561 atgccgcttc agggtcgaga ccgcgagttt ccgctcgagt tcatgaggct cgacgagcaa
     1621 aatggtctct tgccctttcg cggcgacgga ggattcagcc ggctcgttcg gaaccatccc
     1681 ggccgccacg gcgggaagat agacacgcac cgtcgtgcca tggcctggct ggctggcgac
     1741 ctccacctgt ccgccgtgct gcttcaccac tccatacact gacgtcaatc caagtccgac
     1801 gttggtctcc ttcgtcgaga aaaacggctc aaacatccgt gactgcgtat cccgtgtcat
     1861 cccgcttccg gtatcgcaga cctggattcg aacggccgac gcacgtctgt tcacgctggc
     1921 aagggccttg acggctgctc ctccttcgca cgaggaaatc gaaatggtca gacgcccccc
     1981 tcccgtcatc gcatcacggg cgttcaaagc cagatttaac aacatctgtt caatgccttc
     2041 gcgatccacc tcgacatcca gcgggctgcc ttcggccgaa atttggcact cgatccggtt
     2101 cggcaagagg tctcgaatcg tttcactgag atcttccatg agggtccgca tggacagcac
     2161 atgacggaat tgccgatgct cgatatcgag cgttgccaac tgagctgtca gcgcagctgc
     2221 ccgttctccg gtccgaaaga tatcatcgac cgccccatac aaaggatctt ccggaaggag
     2281 gtgcgacagg accactcccg tctgctttcc gatgaccgaa aagagatgac tgaactcgtt
     2341 ggccaggccg gtcgtcattc ggccaatggc ttccatcttt tgtgcctcga ccagctgccg
     2401 ctccaacccc tgccgctccg acttctcttg ctggaccagc gcattggcct tcgacaaatc
     2461 ttccttgagc cctttgatgc gggtctccaa ttggtcgcga gacgcctcca aagcctgttc
     2521 ggtctttttt aatgcgtcaa tgttgtgcag taactgcatt ttttgctgcc gctctgctcc
     2581 gatggtttca agcgccatac cgtaaaaaat tgccatcacc agtaacaccg gtacgcccaa
     2641 caattgaccg actgacagga cgcctgtctg gacgcttccc tggtacaaca caaatccgta
     2701 tcccgcacag agtaacaacg aaaaggcgat cacatgagac agccgacgca gggacgctgc
     2761 gatcagcatg aggatgaaat aggacaaata cagttctgat ctggcctgtc ccgcgagata
     2821 catggtgccg gtcaccagag ccgtgtcgac cgcgatgagt gtaccgctga accaggccgc
     2881 ttggagcacg gaaaagggaa cggccagtag gcaaccgatc acgaccacca atccacccac
     2941 gatgaactcg cttgccgtcc gactgacgat gagctccggc ccgaacaaca actggtagcc
     3001 cagcatgatg gcgaccagca actggaggat cacatagcgc ggccgtgaaa cggctccctg
     3061 atcgtctgtg tcgtgggatg aaatggagtc ttgagaaaag gccagcgtac cgagcattgt
     3121 tcctccccct gcagtgccgg gcaagacacg agcaagtcct gtacccttgc cctcgtccag
     3181 aaggaacttg attttgctga gctctagggc ggtttgatga ttttggacat acaggacctc
     3241 acaaaatgga acggtcacac ggttccagaa agtccaacta aattgaggag agccaattaa
     3301 gtgcggctgg cgagagaaag acgctgccga agacgcgagc ccctgtcccg agcggtctgt
     3361 acatcatggg ccagaaaggt cacgtgcccc attttgcgcc ggggacgaat cgcacgtttg
     3421 ccgtagtcat gtacgtaggc gccgggaagg ccgagcagcc ggcgacattc gacatccctc
     3481 ttgatcgttt ctgcgtcctc accaataaga ttcaccatga ctgctggatt cgtcagcacg
     3541 acttcttgcg gtggcaggcc agtgactgct cgaacctgtt gttcgaattg cgagacattg
     3601 caggcatcca gtgtgtaatg gccggaatta tgaggtcgcg gggcaacttc attaatgagc
     3661 actcgttgat caggcagaag aaatagctca agacaaaaca gtccgatccc ctccatacga
     3721 gcgaccaccg ccctcgcgag gtcggctgac cgttcaacca cattaaaagg cacctctgca
     3781 ggcacgatgg tcattcggag aattcccgcc tcatgcacgt tttcgactaa gggatagacg
     3841 attgtctgtc catcgattcc acgagctacg agaacggaaa gctcacgctc gaatggaaca
     3901 aagctctcga cgatccattc catcgtgctg tgagacgatg ccatcagaga ttccttgacg
     3961 gaagacacgg acgacggctc atcgatcttc cattggcctt ttccgtcgta gccggcagtc
     4021 gcggtcttgc ataccgcagg gtaaccgagt tgttccagaa tctcgggtaa cttgtcgggc
     4081 tcagtcaacg ccgcgaagcg gggaacgggg aagccctgac ccgcgagaaa agtcttctgg
     4141 acgaggcgat tctgtatcag ccgtaacacg ctgctggctg gccgcactgg tgttgtccgc
     4201 tcaagtcgct cgcaaagcac ggctggcaca ttctcccatt cgtacgtcac aacagatacc
     4261 aggtcggcaa actgcgtgaa cgcttgcgga tcggtgaaca gtgatggaaa ggaatgcgcg
     4321 gcgaggcgat gtgccggcgc atctgggtcg ggatcccaga cggcaacacg gtaatcaagg
     4381 cgtgtcgcgg cctgtgcgaa catggcgccc agttgcccgc ccccaagcac cccgagcacg
     4441 gatccaggct cgaccaattg agagttcaca ggcgacgggg ttgatgcatc gatgacctga
     4501 ggcccgatac ggaacctttg gcctcgggag actgcagcac attggtcg
//
LOCUS       DASTQY010000328        27152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_478449, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000328 DASTQY010000000
VERSION     DASTQY010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 27152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27152
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478449"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFR82_10710"
     CDS             <1..261
                     /locus_tag="VFR82_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502112.1"
                     /translation="EILHHRLFLPMVDEAVRCLHDHIVDQAWEVDFALTYGIGFPAFR
                     GGLLTWARQTMTPEQIARKLDELSHKFGKRFEPFPGLSHGGW"
     gene            298..2025
                     /locus_tag="VFR82_10715"
     CDS             298..2025
                     /locus_tag="VFR82_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEU4502113.1"
                     /translation="MHEQTCVHYPEGVPRELPLCSETLVDMLARSARRFSRRPALYFF
                     GKTVSYRECHDLVERFATGLATLGIRKGDRVALCLPNSPQAVIAYFGILRAGGIVVAC
                     NPTYTEHELSHQLRDAGARAIVVFDQMYQKIQHLDLELIIVTRITDFMTTIARYLYHH
                     RNGHRSPDIPTGGTTLWFHDVLHQATPVEPENLPVVVPDDIAVLQYTGGITGVSKGAE
                     LRHRNLTANIQQLISWFSPAERVESFLAALPLFHVFGMTVTQNICLAVGGCMVLVPDP
                     RKMPVLLTLLRKKQPTVITLVPAFVRKLLEHCDTKDFTATRFVICGGASLSYELLEKF
                     KERTGHMIVEGYGLSEASPVTHCNLPRGLSVKRSIGLPIANTESKIVDAAGQEVPDGE
                     VGELWVRGPQVMERYWNNPAETANVVKPGGWLATGDMARIDQDGFFYIVDRKKDMILS
                     TSGFNVYPAEIEHVLLLHPLVREAAVIGVQVTDGSEAIKAFIVLKAETVSVEDLLAHC
                     RHYLASYKMPKHIEFRTELPQSILGKMLRRVLRVEEGGRLQLNGDTPHRINGQILAGQ
                     NISGCKAGQ"
     gene            complement(1946..3130)
                     /locus_tag="VFR82_10720"
     CDS             complement(1946..3130)
                     /locus_tag="VFR82_10720"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU4502114.1"
                     /translation="METYDVCIVGAGVVGCAIARELGHKFPQKRLRVVVFEQRTLAGQ
                     ETSSRNSGVLHSGIHEYPNSLKARLAREGSALAVSYASQRGIPLLRSGMVIAFSWQDV
                     RRGLWREVSSLRRLWANAWKSNIQVSFVTPNRLKALEPNLRGCAGLIIPSVCVIDALA
                     FVQALRADAEDTGSAFAFNNRVIDIDADSTSYLVTTDRTKIRATCLINAAGLYADDIA
                     SLALRTTKYTIHPLRGEYYEVISPEKRGLIGRLVYPALPRQATGKGIHFSPRPNGQLF
                     LGPNEFAVLDKTDYTSRKTPPDMFIETLQKFLPALNERDLRWAYSGIRPCVMTGQGSK
                     SDFIVSADRENPPLVNLIGIESPGLSAALALARHVTDLPCIQKCFGPQGSGHLSDAVY
                     HR"
     gene            3187..4404
                     /locus_tag="VFR82_10725"
     CDS             3187..4404
                     /locus_tag="VFR82_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU4502115.1"
                     /translation="MSELTRKYGKILFVFCGGGCKAIIQAVAAAELVKAGLTPSHIIS
                     SSAGSCNALGFVENPGPVGAENTIRIWEDYITSPEAIYEVHPFFREKLTRLLGVVPRA
                     TQDWGPSGSILHDLRRAIKFLPLILSFCTRMPLRVAGRTVSFIFRLMDTFESQHKSFR
                     RLVQCPEIQEAFDEVDKFFEFKRMKAFLDPFPLLARLGEQIDLKKVLTSSTVWHILTE
                     RYEDGATVIFSNKDADVAMDANDDARSLKSKYDLFYKRIRASMALYPLFEMVDMEGYR
                     YLDADLANPLPVEEAFSFGCDTVFFFLNVPKKSVRVDPHPLRDLLELNDLLRLNERFI
                     HNRVMEAQLKAKTLGVNLFIVRPEEIPSGLGLLEIDNKVIQSVKMNERKRMKEYLSNL
                     DNHNLRFVPLSDV"
     gene            4445..5137
                     /locus_tag="VFR82_10730"
     CDS             4445..5137
                     /locus_tag="VFR82_10730"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEU4502116.1"
                     /translation="MRRILKIGHRGAAGHAPENTLAAIRKGIALGVDFVEIDVRCTAD
                     GVLVVLHDATVNRTTNGKGPIERLSLRDVRALDAGNGEYIPTLEEVLGVLAGQTGLMV
                     ELKIKGVAKKTVEVVQAAQFNKPVIYASFLHEELGIIRSIDAQASVMLLFGRLPRAPV
                     VHAMKYSPSHVGLRHDTISRRLVKAFHDEDMLVCVYTADRIRDIERAWSAGVDGIISN
                     VPERVDSHQIHV"
     gene            5475..5660
                     /locus_tag="VFR82_10735"
     CDS             5475..5660
                     /locus_tag="VFR82_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEU4502117.1"
                     /translation="MLRWSAVFLVIAIIAAALGYGGIAADAAEIAKVLFFIFLGIFVV
                     TLILGLIAGQKLFGQRR"
     gene            complement(8091..8756)
                     /locus_tag="VFR82_10740"
     CDS             complement(8091..8756)
                     /locus_tag="VFR82_10740"
                     /inference="COORDINATES: protein motif:HMM:NF042831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HEU4502118.1"
                     /translation="MRRFLYENSLSLAMLALFLFFLAGQSVAGWCVYNSDQQDHQQSP
                     IPYGAYLLSGHFFEAVFENWESEFLQMSAYVIFTVYLRQKGSPESRPLDSKDTHEDSV
                     SAARRRPHSPWAARQYGWIRKMYEYSLTLALFLLFIFSFFLHAVAGQQVFNEEQALHG
                     GHAVTLGGYIMVSQFWFESFQNWQSEFLSVGVLIVLSIFLRQKGSPQSKPVSHPHGET
                     GDE"
     gene            9502..10125
                     /locus_tag="VFR82_10745"
     CDS             9502..10125
                     /locus_tag="VFR82_10745"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEU4502119.1"
                     /translation="MTTLRRIGIPGTSTASWGEHLCAFFYTKAHLLDLLVPYIKTGLE
                     DNEYCLWITGESVMKEEALHALEGIMPDAATYLASNQLAIISYREWYFSSGVFNPQKS
                     FDNWAARAQCAKANGFSGTRTTGNPFWLQSEEDWTRFLDYEHIVNRAIETQRVISLCT
                     YPVDICNSKNMLSTLASHHAMLLSHGDEWKCLDLRGRGAMTQADHRN"
     gene            10828..11313
                     /locus_tag="VFR82_10750"
     CDS             10828..11313
                     /locus_tag="VFR82_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502120.1"
                     /translation="MQLGAIASRTLIIPLTAGALALIGTVSTPVVQAAAPEEKIEIAI
                     SDQGYNVKGHTTPGAVTAIVVRNQGSMTHGISSPLFKASVIKKEGDGVEIRGPKGKGF
                     RAYHLEPGQTMTLHFKKEGSTDPATGISETIQVPFWCDIHQHMKGEFLVIETKGEVGG
                     G"
     gene            complement(11767..12129)
                     /locus_tag="VFR82_10755"
     CDS             complement(11767..12129)
                     /locus_tag="VFR82_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU4502121.1"
                     /translation="MFLRNPLLAAIALPVFLMGQPSLIQASSADVAAGKRIYLSLCKA
                     CHGLDGKGPGVMKFNPPAADLTSPQVQTKLDSRLYNSIHEGRVNTAMGAWKYELSAKE
                     ILDVMAYVRTLSDVPSTP"
     gene            complement(12208..12627)
                     /locus_tag="VFR82_10760"
     CDS             complement(12208..12627)
                     /locus_tag="VFR82_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502122.1"
                     /translation="MSTRSKSAILMFLVLGPLALFTAPPVDSQSEQVVEVVIKGFSFI
                     TKQEVLRLGMPTVIKIVNEDTERHDFGSTMFEGIQARIEKDGVIVYGRGLGGVMLDRK
                     RDATIRFIMERPGRHTFRCSIHPDMKGELLLLSAEAV"
     gene            complement(12726..13790)
                     /locus_tag="VFR82_10765"
     CDS             complement(12726..13790)
                     /locus_tag="VFR82_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU4502123.1"
                     /translation="MSTMRANVFQAKNKFQVMEVPRPKVADGEVLIRITLTTICGTDL
                     HIVRGEYPVPAGLIIGHEPVGIIEDLGPGVAGYQVGDRVLVGAITPCGQCNACLSGHQ
                     AQCGHGSGYQAIGGWRFGNTINGAQADYLVVPYAQANLAKIPDDVTDEQVVLLADIAS
                     TGFSGAESGGVKIGDSVAIFAQGPIGLCATVGARLMGASLIIAIDGDESRLRMAKRLG
                     ADIVLNQRETNVVEDVKRLTDGGADVAIEALGLQETFENALRSLRPGGTLSSLGVYSG
                     KLQIPYEAFAAGLGDHRIVTTLCPGGKERMRRLIEMVRHHRIDLTPLLTHRVSLDNIH
                     EGYRIFGERLDGVLKVAVKP"
     gene            14117..15496
                     /locus_tag="VFR82_10770"
     CDS             14117..15496
                     /locus_tag="VFR82_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU4502124.1"
                     /translation="MLDIARNVLVVDDDHEMRALLRDVLEQHGYTVSLASNGQEALVN
                     LREAEYPVVLTDLRMKGMQGIELLAQIKQSWPETNVILMTAFGSVETAIQAMKQGAYD
                     YLMKPVKNDDLLRATERGFREALLRSEINRLRREVDKEYSFSQILGKSKAMREVFDLI
                     RRVANSPTNILITGESGTGKELVAKALHYNSERKDAPFVPVNCAAIPEHLLESELFGH
                     VRGAFTDARADKRGLFEEAEKGSILLDEISELPHMLQAKLLRAIQEKEIRRVGSTKST
                     AVDVRVIAATNLNLAEEVKAKRFREDLYYRLNVIEVKLPSLRERREDIPLLVDSFLKK
                     FGQARGKDVKGMSEAALAILMDYAWPGNVRELENVIERAVTLSRADRILPEDLPSTIQ
                     GVRGDRRVVDEGAEKMVPLHEVEKEYIKKILEKMGGNKYQAAQVLGIDRKTLYRKLAE
                     IEGTLPPDE"
     gene            15520..17079
                     /locus_tag="VFR82_10775"
     CDS             15520..17079
                     /locus_tag="VFR82_10775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4502125.1"
                     /translation="MKVVKEVDIRRVSWAIPVLIISVFVFDLAAPVGVAVSVLYVIPL
                     LWTVHTARQRDALYYCVAATALLWTALLLKPSALLLDFSVFNRMLGTFVLCLLTWGLL
                     HLKRTQHELLQSEMRRTKAVQDLMAERVERAHAEGLMEVAQEARAYAETAVTGAVAGR
                     QRAEEQYVISKLRLEGIIDSAMDAIITVDHEQKIVVFNHAAERMFKCPAQNAIGQPLD
                     RFIPAHLREAHRSHVNRFGQSGVTNRKMGQLGTVTGLRTDGEEFPIEAAISHVMVQGT
                     KFFTAILRDITERTQLEEQLRRTERIAELGTVASGMAHEIGTPMNVILGRAEYLLDRV
                     TDEPVKKGLQTIVAQVERITRVMNQLLSFARRKPPRPGPLRLQEVVENSLEMFHERLS
                     NHQVQVDLQVNAGSPNVLGDSDQMSQVMINLIMNALHAMPDGGMLRITLGSEGGMAKL
                     TVSDTGHGIPDDVVSKIFDPFFTTKEFGKGTGLGLTVVKGIIEEHHGSIEVESEPGKG
                     TTFTVLLPKSS"
     gene            17264..17671
                     /locus_tag="VFR82_10780"
     CDS             17264..17671
                     /locus_tag="VFR82_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502126.1"
                     /translation="MPTMAVHKKAVVLVVEDDADMRNLLCDAFWSAGYQLLEAKDGDE
                     AFLAVLQSVPDLILTDLRMPAGGADYISRLRTVAPHCPIVVMTAFGEPGLQTEVLSAG
                     ATAYFDKPVRISDLKLRIEALLTRSPVTDEERA"
     gene            17673..19061
                     /locus_tag="VFR82_10785"
     CDS             17673..19061
                     /locus_tag="VFR82_10785"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU4502127.1"
                     /translation="MVRSSSPDACSQRDPVLTARIETISQLGESLSDRIAIMDRQFNI
                     LYTNHTYRPAEASTRSPSEQTKCYHIFARRTDRCAVCPAAQAFENPSREGSSCAIGSE
                     TRACGIQRAVPLLGENRQVASMLILLEKPAPSNGLARMPASEEAAPPTVREQLDELIG
                     RSPAMQQLFDMITLIADSSATVLIQGESGTGKELVAKSIHRLSNRRRKPFVVVDCGSL
                     PETLLESELFGHVKGAFTGAVANKRGLFEEADGGTILLDEIANTSQTFQAKLLRVLQE
                     GEIRRVGSNQPIKIDARVISATNKNLTELVSVKQFRQDLYYRLAVLPLYLPPLRERKE
                     DIALLVERFLMQSCAKHGQPGRHVSDEMMCRLTESAWPGNVRELKHYIERAVITTIGP
                     ALTCQDIVAKRTDSPALDLRSVSRGAMRQVERAQIVQALALTHGNRLKAAKYLKISRA
                     SLYNKIRSYSIR"
     gene            19495..24435
                     /locus_tag="VFR82_10790"
     CDS             19495..24435
                     /locus_tag="VFR82_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502128.1"
                     /translation="MKSFIEQSQGKRRWGSLVLMLPTVTLTCGLTATPWLFAASTGEA
                     SHEGHATAVAMPGWTQQLKGQTVVENAIEGRAGNAEKMEMQHHRLMQKLEQQAQEDAK
                     AQQTSGAFNNMSMMHQYMGQDGSSFLLMTDSSKGEPVSALGGKCPSNVPVRMYDVSMI
                     NIEITLNRWLDYYPGYMYVLTEDVEKVRAEETRNKDARDKEGFDPGSVTTGLQGDMIQ
                     PLVLRANQGDCVRMTLRNQMESEDGSLFIQASSMIVSATGKPATTTNPESIISPGKLQ
                     EFEWYIHPQMQEGVRQFHSYSHDRELTVLGLFGAFVVEPKGSKYLDSIGTGSDKQVKT
                     GWQVNIDNGSGPDFREFVLFYHEVGDEAFRPLNKKGDFLPQRDPLTDAYRPGGRAINY
                     RSEPFGIDQMHLQHEYFGFEDESMAYSAYTFGDTPTTIARGYLGDPVKWRLVHGGSEV
                     FHSHHPHSGSIRWQRSPGTETNNMWAMGQDGPVKYPVVRTKSDRVDVEVIGPSEALDL
                     EPECGGGGCQHLAGEFLYHCHVAHHYVAGMWGYGRFYNTLQVGAAHTDTMPDLQELPD
                     RKGRMKVGVTSDKLIGTTVDWFGKSFKIVDKGQKTNWKSNPVIVNIKDWVEMFVPAQG
                     KPGHTDKEKDQILAYDSTVRDWKWDGNIARGERESTAQNPKYPWAAKWDDSTRPAILF
                     DPTTGKMAWPLFKPHFGKRVPFSANHSGAPWLEPIHQDANGERTSEPAKPGEQGRWSL
                     CPDNANQKFYNLHFLRMPITLAKQQGKEPPIVDKDGLIYVLHEEERLTRGNDDLKLPA
                     VIRANVYDCVDLVLTSEWDDDDYTNFQSSKINIHPHFFQFDTGNSDGVISGFEYEMSV
                     RPFTMWGKAKKHGLPAPMVAKIVGGAKAGASSIKIQLAPGATPFHVNTEVMVGVDCLE
                     KGNDPTASLPRDKSCSEVARIKDIKGDQVTFFKPLKHNHPASDLVSPEFVRYRWWVDV
                     DMGTVFWHDHAFGATTWPHGGFGVTIVEPFGSTYHNPKDGKLVYSGPVADIHSNEPIG
                     AGVSGSFRELMVSIHDTVPHTVNVIEAGNPPGQPVEVALEAGKTVSFQMPEKILNAPN
                     KYINGGTHTTGSGFNFRAEPFAQRLSNNPDTSKLFSSAIHGDPDTPLLRAYTGDTMVF
                     RLLHQLMNESHVWTIAGHTFLTERYAADANRKNSIHVGIAERYDLVTKAGGFQGMPGD
                     YIHFNGRSSHFAEGGWGIVRVLDKETSDLKPLPKGTNPLGIPTTPSGVCPADAPVKSF
                     TVVALDRPLKLHPKAPEAIEVDFERKIELTVPEGKIFALEEEATTVAGNVMPNPLTLR
                     ANLGDCIKVNLKNKMKASRASFFAPGLAFDPKDSQGLNVGNNGGDQTIGPGESRNYTY
                     YAHPSNKETTSLVWDGGNIVVNPRNGLYGAIIIGPKGSQYRDPVTGADLSQKNAWRAD
                     VVVDTSLPENVGKRNYRDVALFFQDEDNIIGTSFMPYVQNVAGLTSVNYRAEPYKYRE
                     EQGCSLGKIFQPCVVDKPEDPATPLIEAHAGDPIRIHVIGANSEQNGMFGVEGHEWPI
                     EPYMPGADMISVVEYAGSEVLDVFIQGGAGGPYRQTGDFVWSNNRLPYAQSGQWGYLR
                     VLPTGDSRIQPLGAASAGAKRADARPAPRATPTAMK"
     gene            24723..24938
                     /locus_tag="VFR82_10795"
     CDS             24723..24938
                     /locus_tag="VFR82_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502129.1"
                     /translation="MEKNEINKWRRWEMAVNVLLVAVALVTIANAAWGLQTPDVTAWA
                     PKGTPPVGSSHVLIAARRDEMPANRTP"
     gene            complement(24943..26340)
                     /locus_tag="VFR82_10800"
     CDS             complement(24943..26340)
                     /locus_tag="VFR82_10800"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4502130.1"
                     /translation="MKLTFHGAVRSVTGSRHLLETDGNRVLLDCGLFQGRREETERRN
                     RYLGFDPKEIDAVFLSHAHIDHSGALPALAKYGFSGKVFATRATAELSEIMLTDSAHI
                     QESDCVYVNRKERRTEGMCRQPFYTSDDVQTIKRQFVGLRYGDVVTPLPGMKATFLDA
                     GHILGSAAISLRIMEAGATKTILFSGDLGRRQMPILRDPQPPPSCDVLIIESTYGDRL
                     HSETADEAKQKASALIHHAIATRSKIIVPAFAVGRTQDLVMWVKDMVKEGRVPPVPIY
                     IDSPLALRATEVFRRHPECYDEETYRILTTEGDPFIAKYIRYVSSVHESQNLNTMRGP
                     CVIIAASGMCEGGRVLHHLRHAIQDETNVVAIVGFQAEHTLGRKLIEGWDPVPIFGVP
                     VPRRARVVHFNGLSAHADREDLLGYVRAINPLPSQIFIVHGEHRQAFSLAAAIAAEHP
                     HVDVRVPEANTAYEV"
     gene            complement(26337..>27152)
                     /locus_tag="VFR82_10805"
     CDS             complement(26337..>27152)
                     /locus_tag="VFR82_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEU4502131.1"
                     /translation="RADQDLAFLNGDEVRPIRLQLELLKTELIQRRENIRSTIVVFGS
                     ARIPEPAVSNRRLDEANKRLAGKPHDVSRQRSVAMAKRQVAMSKYYNEARAFGRLVTS
                     KYQSKRGCDYVVVTGGGPGIMEAANRGAADVKGKSIGLNISLPHEQQPNPYITPALCF
                     QFRYFALRKMHFLLRAKALVAFPGGFGTCDELFDALTLLQTKKVRGITVILVGREYWS
                     RLINWQLLEDNGFISARDRKLFQYADTAEEAWKLIARTQQTFRRRPPPPRWFQ"
BASE COUNT         6343 a         7276 c         7539 g         5994 t
ORIGIN      
        1 gaaatccttc accaccgtct cttccttccc atggtcgatg aagcggtgcg ctgcctgcat
       61 gaccatatcg tggaccaggc ctgggaggtg gatttcgccc tgacctatgg gatcgggttt
      121 cctgcgtttc gcggaggcct gctcacgtgg gctcggcaaa ccatgacgcc tgaacagatt
      181 gccaggaaat tggacgaact gagtcacaag ttcgggaagc gctttgagcc ttttccggga
      241 ctgtcgcacg gagggtggta acgacaagtc ttttcactga acgaaacgga gcgcgtcatg
      301 catgaacaaa cgtgcgtcca ctatcccgaa ggagttcctc gagagctccc tctctgttca
      361 gagactcttg tggacatgct cgcgcgcagc gcgcgacgat tttcacggcg accggcgctc
      421 tatttcttcg gtaagaccgt gtcgtaccgt gaatgccacg acctggtgga gcgtttcgca
      481 accggtcttg caaccttggg aattcgtaag ggagaccgcg tcgcgctgtg tcttccaaac
      541 tccccccaag ccgtgatcgc ctacttcggc attttgaggg cggggggcat cgtcgtcgcc
      601 tgtaacccga catacacgga gcacgagctc tcccatcagc tccgagatgc cggcgcgcgg
      661 gccattgtgg tctttgacca gatgtaccag aagatccaac atcttgatct tgagctgatc
      721 atcgtgacgc ggattaccga tttcatgacg acgatcgccc gctacctgta ccaccatcga
      781 aacggccatc ggtctcccga cattccgact ggaggaacca cgctctggtt tcatgatgtc
      841 cttcaccagg cgactcccgt ggagccagaa aaccttccgg tggtggtgcc tgacgatatc
      901 gctgtcttgc aatacaccgg cgggattacc ggcgtatcca agggagcgga gctccgtcat
      961 cgcaatctga cggccaacat tcaacagctg atcagttggt tttctcccgc tgagcgtgtg
     1021 gagagctttc ttgctgcgct gccgctcttt catgtgtttg ggatgaccgt cacacagaac
     1081 atctgtctgg cggttggtgg atgtatggtg ctggtccctg atcctcgcaa gatgccggtt
     1141 ctcctcacgc tgcttcgaaa gaaacagccc accgtgatca cgctggtgcc ggcctttgtg
     1201 cgaaagttgc tagaacactg cgataccaag gatttcaccg cgacccgctt tgtgatttgt
     1261 ggaggggcct cgctttccta tgagctgttg gagaagttca aggagcgaac cggtcatatg
     1321 attgtcgagg ggtatgggct cagcgaagct tctcctgtca cgcactgcaa ccttccacgc
     1381 ggtctctcgg tcaagcgatc catcggactt cctatcgcca atacagaatc gaaaattgtg
     1441 gacgcggccg gccaggaggt gccggatggg gaggtgggtg aattgtgggt gcgaggacca
     1501 caagtcatgg aacggtattg gaacaaccct gccgaaacgg cgaatgtggt gaaaccgggg
     1561 ggctggctcg cgactggcga catggcgcga atcgatcaag acgggttctt ctacatcgtg
     1621 gacagaaaga aggacatgat cctctcaacc tcgggtttca atgtgtatcc tgcagagatt
     1681 gaacacgtcc ttctcctcca cccgctcgtg cgcgaagccg ctgtgatcgg cgtgcaggtt
     1741 acagacgggt ccgaagcgat caaagcattt atcgtcttga aggccgaaac agtcagtgtg
     1801 gaagacctgc tcgcacactg tcggcactat ctggcgtcgt acaaaatgcc aaagcacata
     1861 gagtttcgca cggaactgcc ccaatcgatt ctcgggaaga tgctgcgtcg agtgctgcgg
     1921 gtggaagaag ggggacgact gcagcttaac ggtgatacac cgcatcggat aaatggccag
     1981 atccttgcgg gccaaaacat ttctggatgc aaggcaggtc agtaacatgt cgagccagag
     2041 ccaaggcggc ggagaggccg ggcgattcaa tcccgataag gttgacaagc ggtggattct
     2101 ctctgtctgc ggagacgatg aaatcgctct tgctaccctg tccagtcatg acacagggcc
     2161 tgattccgga ataggcccag cgaagatcgc gttcgttcag ggctggaagg aatttctgca
     2221 gggtttcaat gaacatgtcc ggcggcgtct tccgcgacgt atagtccgtt ttatccagga
     2281 ccgcgaactc gttcggtccc aggaacagct gcccgttggg gcggggactg aagtggatgc
     2341 cctttccggt cgcttgacga ggcaacgcgg gatagaccag acgcccaatc aggcctcttt
     2401 tttccgggga gatcacttcg taatactctc ctctaagcgg atggattgtg tactttgtgg
     2461 tgcgtaaggc gagggaagcg atgtcgtcgg cgtacagtcc ggcggcatta atgaggcagg
     2521 tcgctcgaat tttagtgcgg tcggtcgtga cgaggtacga ggtggaatct gcatcaatat
     2581 caatgacccg gttgttgaaa gcgaacgcag agccggtgtc ctccgcgtcg gccctcagtg
     2641 cttgcacgaa ggccagagca tcgatgacgc agacgctcgg aatgatgagg ccagcgcagc
     2701 cgcgtaaatt gggctccaat gccttgagcc tgtttggcgt gacaaatgag acttggatgt
     2761 tcgatttcca ggcattggcc caaagtcgtc gcagcgaaga cacctcacgc cataggccac
     2821 gacgtacatc ctgccatgag aacgcaatga ccattcccga tcgcagcaag gggatgccac
     2881 gttgtgaggc gtacgagacc gccagtgcgc ttccctctcg agccagccga gctttcagcg
     2941 agttgggata ttcgtggatt ccgctatgga gtactccact gttgcggctg ctggtctctt
     3001 gtcctgccag cgtacgttgt tcgaacacca cgacgcgaag ccgcttttgc gggaacttgt
     3061 gccccaactc ccttgcgatg gcgcatccaa caactccagc acccacgata cagacgtcgt
     3121 atgtttccat ctggcggggt cctttagggc aaagaacagg cccagccgac aaaccggagc
     3181 atgtgcatgt cggaactcac taggaagtat gggaaaatct tgtttgtgtt ttgcggaggc
     3241 ggctgtaagg ccattatcca agccgttgcc gccgcagaat tggtgaaagc cggcctgact
     3301 ccttcccaca tcatcagttc ctccgccggt agttgtaatg ccttgggatt tgttgaaaat
     3361 cccgggcctg tcggggcaga aaacactatc cgcatctggg aagactatat cacttccccg
     3421 gaggcgatct atgaagttca cccattcttt cgagagaagt taacgcgtct gttgggagtc
     3481 gttccgcgag ccacccaaga ttggggacca tccggatcga ttcttcacga cctccggcgg
     3541 gctattaagt ttttacccct catcctctct ttctgcacgc gcatgccgct tcgggtggcg
     3601 ggccgtaccg tgagcttcat ttttcgcctg atggacacat tcgaatcgca gcataaatcg
     3661 ttccgacgac tcgtccagtg tccggaaatt caggaagcgt tcgacgaagt ggataagttt
     3721 tttgaattca agaggatgaa ggcctttctc gacccgttcc cgcttctggc caggctcggc
     3781 gagcaaatcg atctaaagaa agtactaacc agttccacag tgtggcacat cctcactgaa
     3841 cggtatgaag atggcgcgac ggtcattttt tcgaataagg atgccgatgt cgcgatggac
     3901 gccaatgatg acgcgcgaag tctgaaaagc aagtacgatc tcttttataa acgtatccgt
     3961 gcgtccatgg ccctctatcc gctgttcgag atggttgaca tggaggggta tcgatacctt
     4021 gatgccgacc tcgccaaccc gctccccgtt gaggaggcgt tcagttttgg atgcgatacg
     4081 gtgtttttct tccttaatgt tccgaagaag agcgttcgag tcgacccaca tcccttgcga
     4141 gaccttctcg agttgaacga tctcttgcga ttgaatgagc gctttattca caaccgtgtc
     4201 atggaagcgc agctgaaagc caagacattg ggggtgaacc tgtttatcgt ccgtccggag
     4261 gagatccctt ccggcctggg gctgctggag attgacaata aggtcattca atctgtgaag
     4321 atgaacgaac gaaaacgtat gaaggaatat ctctccaatc tagataatca caatcttcga
     4381 ttcgttccac tttctgatgt gtagttctcg ctgggcttcc tcggtctcgg ctccaagtga
     4441 tccggtgcgt cgcatactca agatcggtca tcgaggagct gcgggacatg cgccggaaaa
     4501 caccttagca gcgattcgaa aaggaatagc tctcggcgtg gacttcgtag agattgacgt
     4561 gcgatgcacg gcggacggcg tcctcgtggt gcttcatgat gcgaccgtca atcgtacgac
     4621 aaacggaaaa gggcctatcg aacgcttatc gctccgagac gtcagggctc tcgatgcagg
     4681 gaatggcgaa tacatcccca ccctcgaaga agtcctcggt gtgcttgcag gacaaacagg
     4741 tctgatggtg gaactgaaaa ttaagggagt ggccaaaaag actgtagagg tcgtacaggc
     4801 cgcccagttc aacaagccgg tcatttatgc atccttcctg cacgaagagc tgggaattat
     4861 acgatcgatc gatgcacagg cctctgttat gttgctcttc ggcaggttgc ctcgtgcccc
     4921 tgtcgtccac gcgatgaaat acagcccttc acacgtcggt ctccgtcacg acaccatttc
     4981 ccgacgcctc gtcaaggcgt tccacgacga ggacatgctg gtatgtgttt atacagcgga
     5041 tagaatccgt gacatcgaac gtgcatggtc cgccggcgtt gacggaataa tatccaatgt
     5101 tcctgagcgg gtggacagtc atcaaattca tgtttgaatg cgggctcatc agtcacttgt
     5161 atcgttaacc tctactcctg ataactactc taatcttgtg cgccggtttc tacctcaggt
     5221 ggtcaatcct gccgctgact ctgttgcaag gtagccctgg ggccgtctca ataatgtccg
     5281 atgaggaaaa gagattccat gactagcatc ccaaggagtt catcaatggg tacttccata
     5341 gtgttgggtg ccaagttcaa gggaaaaccc taattaataa actgtgtccc agcgtttatg
     5401 ctaatccagt tgtagggggc agaggtgaca tcacttcctc cgacgatggc tcactcttaa
     5461 gaaaggagat ctatatgctt cgctggtcag cagtcttcct ggtgatagcg atcattgcgg
     5521 cggcgcttgg gtacggcggc atcgctgctg atgctgctga gattgcaaag gtgctgttct
     5581 tcattttctt ggggatcttc gtcgtgacac tcatactcgg attgatagct ggccagaaat
     5641 tattcggtca aaggaggtaa cagcactagg agtgattctg tagttttcgc ctctgaacga
     5701 ctccgaacga gccaccttcg cccttgtgtc cggagggcgg aggggctgtc tcggctcgct
     5761 tccgagtgag ccttcatttg taatgttgtt gccgcttact cgatctccta gctttccgag
     5821 cacaccgtca tccctgtggt attgcgagta atcttctccg taagccgagc cagtcttccg
     5881 cgttagatcc aaataggctc gatcaaattg tcgccgcgtt cgtgccgcag catcacggat
     5941 cacgcaagag aacgggtaaa gtgtgaggga ttgaagctat ggagggtgga aggagattag
     6001 gacgatgggg gatacagctt tcttggcccc agagacggta ggagagttac ccagagcgcg
     6061 cgagccacac tgcagaactt cattcgcggc ggtggaatga tcgaattctg ttcagccact
     6121 ccttcataat gtttcgccgt ttcataggac tgagggccga tcgagctttg catcgaggtc
     6181 gactcatgca gtccggtagg cggcaaggag gagattcgga attacgtggc cgatggttcg
     6241 actgccgttg cctctgggta ccgtaatcgc acagtcggct tgtctgagag atattctcga
     6301 gacagttcgt tcaggtagta ttcgagaagg tcgcgctcct cgtcagtcaa ccgctcgggg
     6361 agggaaagta aatgctcaca cgcacgtgtg taggctctta tttcttggcg gagattcata
     6421 acgcaccagc ctttcaaaac ggggttgatg ttgtgaaaga tggcggtgat gagcggatga
     6481 aaatatgcac tagtatgtct aacggtatgc agccggtcaa gggttttgcc cgtaggattc
     6541 tgctgacagt tgtgtcagtc aaaaagtaca acaaaccatt gaaaaattaa gatatattgg
     6601 cgagatggaa cctacggtca taaacaattt ttataaggcg acacaaggtc tccgcgaggc
     6661 gatggaatgc gatctgcctt tgaatgatct cgaacgcatt tccctagaaa attatattgc
     6721 cctgcttcaa atgacctaca tcgaatggaa gagaaggaac tgccagccgc ccgcctatag
     6781 aaaagcggct taaacgttcg tcctgccagc ccggtcgatt cccactcatt tgcaccgcca
     6841 tccccgtaag cctgtccttg aactgctaag cctcgaatac gcggcagtac gtacagaatt
     6901 tgtacgtaga agcaggtatt ttgttcattt ttgtctcacg gccgatccta agtagttgaa
     6961 actacgtcac tccggccagg ggtcaaaaat tgctatgtga ggtcatagct tttaacctgg
     7021 cccggaggat ttatggattg cccaaaatgc aatgggttaa tggatttaga gcggttctcg
     7081 gatttttttc tcgtcttcta tgcctggaag tgtattaact gcggcgccgt tatcgacaag
     7141 accattgcta tgaatcgaag aaaaagcttg gcagccaaac aagagctcac caaggtctgc
     7201 gttgatgcta cataggtaag ggttgctacc tcgctcttga gaaggaattc aggccggagg
     7261 tgctgcatgc ggtctcacaa caacaggcct ttcaggaatg tggagaaagt tgccgtcgcc
     7321 gcggagtttg tggtcgcggc tgaacggctg attctccatg atgtcaatcc tgagctcctt
     7381 ccagagcgtg aacgagcgac tctcaaatac taccttgatt gcctttcgaa gaagtttagt
     7441 gaaggccacg taaaggagcc aagctgatca cgaacagtat ttcatctctt aggaagacct
     7501 gcctgtcgga tcggtgtcgc tgagcacgac tcttccttca atgcttcatt ctctccttct
     7561 gctgaacaag aattagtttg ccgctcgcca tctttccatt gacacaggtt accatcgaca
     7621 ctatcaaccc cccgtatcga gagagcatcc gattgtaacc gggactgcca ggaacagcga
     7681 gtgtaaggat ctcgtagaag ggcagggaac caccgggatt tcatacttcc ggcaatcccc
     7741 gtctcaggcc tatccgcaag gccatgagca aagcaagggc cacaaagcct cccggatcta
     7801 tgcgacctgt cgtttgcttg tcggctggtc tttcgagtcg ctctgattgg ctacagggtg
     7861 cgagtcaccc tttctcggac gggctcggcg aaactcggcc acagccttcg ggcctacaaa
     7921 gagaacgccg atgatacagg ccagcagggc tacaattttg agcatgagaa aaacccgctt
     7981 ccaggtacgt atacaaggaa ttttacaact gcggcaaaga ttcgtcgcct aggaacccct
     8041 ctggcaatgc tgaccgaaag gcgtatgttg cgctgcttca ggggatcgtt ctattcatcc
     8101 cccgtctcgc catgtggatg actgacgggt ttagattgag gagatccttt ttggcgcagg
     8161 aaaatggata aaacgattag caccccgact gagagaaact cactctgcca attctggaat
     8221 gattcgaacc agaattgtga gaccatgatg taacccccca gcgtcacggc atgtcctccg
     8281 tggagggcct gctcctcatt gaacacttgt tgaccagcga cagcatgaag aaagaaagaa
     8341 aagataaaga gtaagaaaag cgcgagggta agagaatact catacatttt gcgtatccat
     8401 ccgtattggc gtgcggccca ggggctatgg ggacgccttc tggcagcaga aacactgtct
     8461 tcatgtgtat ctttactatc aagtggcctg gattcgggag atcctttctg acgaagataa
     8521 actgtgaaaa tcacataggc gctcatctgc agaaactcac tctcccagtt ttcaaaaaca
     8581 gcttcaaaga agtgaccgct taggaggtaa gccccatagg gtatagggga ctgctggtgg
     8641 tcctgttggt cactattgta gacacaccat ccagcaacgc tttgaccggc caagaagaac
     8701 aggaagagtg caagcatggc cagtgaaaga ctgttctcat acagaaatcg tcgcatggtg
     8761 cctcgacttg ttctatttat ggacgactcc ttagtttctt cagggtaaac tggttccgta
     8821 acacgcagca ctgggaaaac accagaatta gtaatggagc gccttagcgg ggtaatccgg
     8881 atccaagctc tccaagcgtg cctatgcgcc attggcgttg atgataacgg acgagatccc
     8941 attcaacaaa attgagccag cgctgtggat aagtcattga cacggtcctt accttcaccg
     9001 attccatcaa gtttttcttt ccaccgtctt tacattttac ttcgcttgct aaatccataa
     9061 gtcacaatgg ctgacacaac atcttgggtg gattgtttac agagcactat gcctaaaaat
     9121 aaggcaatct gtcagttagt agatctagct ccgcgtctca cactctgaca ggcaagctat
     9181 ccacaagctt atccacagat cctgtggtca gtgtttgctt tcaaagatgt ttcgggcgtc
     9241 ggaacgccta agatatcccg aggagggcag ttgacggcgg gcttttgagc acatagagga
     9301 gagtcttgcg tagctaacca cctgtctacg atgtaaaccg caacgcttgg atggacgtcc
     9361 agaatgcaat cactttgact gaccgtcatg tgtagcgtcc ttccaccatt gacagtcatt
     9421 gctcacgcgg gtaatgtgcc gcacatccaa cacgagatca ataggtggca ggtactctgc
     9481 aataagggag aacacagctt cgtgaccacc cttcgacgca ttggcatacc cgggaccagc
     9541 accgcgagtt ggggagaaca tctatgcgca tttttctaca ccaaagcgca ccttctcgat
     9601 ctacttgtgc cttatatcaa gacaggttta gaagataacg aatattgcct gtggatcacg
     9661 ggcgagagtg tgatgaaaga agaagctcta catgcgctgg aaggaatcat gccggatgcc
     9721 gcgacttatc tggccagcaa ccaactagcc atcatctcct atcgtgaatg gtacttttcc
     9781 tctggcgttt ttaacccgca gaaatctttt gataactggg ctgctcgggc acaatgcgcg
     9841 aaagcgaatg gcttcagcgg tacgcgaacc acgggcaatc cattctggct gcaatcggag
     9901 gaggactgga cacgctttct tgactacgag cacattgtga atcgtgcaat cgaaactcaa
     9961 cgtgtgattt ccctttgcac gtatcccgtc gatatttgta atagcaagaa catgttgagc
    10021 accctggcgt cacaccacgc catgcttctt agccacggcg acgaatggaa atgcctcgac
    10081 ctacgcggcc gaggcgcaat gacgcaagcc gaccatagaa actaacccca cgtttctcct
    10141 ccttccttca ggttcaagtc ccagacgaga ccttctgtaa atcaatcaag cgcctgtctg
    10201 ggagggagaa cgctaatcgt tccctctgat ggttgattga gaagtgaggc gaacgctgat
    10261 caccagatat ggtctgtcgc atttgacagt gcgcctgtat ttgacagtcc tcacttaaca
    10321 atagttctta tacaatctcc taccagcaat tgatcgcttg ttggtggctg ctttcccgtc
    10381 taatccgcaa cgaccctaag ctcacaacga ttgatgaccg atttacaggg gaatccataa
    10441 cttacggtga gtgctaggtc gatatgcatc ggcaccgacc tcagaatccg caggtccctc
    10501 ctttcatggg ttgagacatg gactacattc atattgagcg tgccattctc aggcatgaaa
    10561 agtaggacca tcgctggaag atcattgagc aacccgatgc aatgagtact ttcgctacaa
    10621 ttagtgaggc gttactcccc aattcccggg atgacaaatc tgtttgaagg cgttcgctca
    10681 tcacaaccaa gccgacttgg ggtgctcgaa gttattcttt gccgatacta cctccggcac
    10741 tgccattgca gtccagttac tccgccgagc agcctaacat ctgccggata aagattcgca
    10801 cttaaataac tacacaagga ggtcgtcatg cagctcggag caatcgcttc caggactctc
    10861 ataattccgt tgacggctgg tgcattggca ctcatcggga cagtttctac gccggtggtt
    10921 caagcagcag caccagaaga gaaaattgaa atcgccatct ctgatcaggg atacaatgta
    10981 aagggacata ctactcccgg ggcggtgacg gcgatcgtcg ttcgcaatca aggctccatg
    11041 actcacggca tcagctcgcc gctgttcaag gcgagcgtga tcaagaaaga gggcgatggc
    11101 gtcgagatca gaggcccaaa ggggaagggg tttcgagcct accacctaga accaggtcag
    11161 acgatgacct tacactttaa aaaagaaggc agcacggacc cggcgactgg aatcagtgaa
    11221 acgatacagg tccctttctg gtgcgacatt caccagcata tgaaaggcga attcttggtg
    11281 atcgaaacaa agggagaggt cgggggcggc tgagtagtcg cagttgctca tcctcagctg
    11341 ggcacgaggt cgtgtggctc gaatcgcttt acggcctcgt gcacgcgcta gtcgctttct
    11401 tcagcagatg cacatcacag gagcggctac tggccccgtg agcacttgcg ctgtaggcag
    11461 gacgccggac agccaacaca gggactgttc ctcgccgcag gacagcctcg gtcacgctcc
    11521 ctgccaacac atgtgagagg cctcggcgtc catgagtgcc catgacgatg aggtcacatc
    11581 aggatcttcg cgcgacttcg agaatcgtgt cagcaggagt gcctcctaac ggtgaggccg
    11641 gcacgccaag aggcagccaa cgcacaggca tagtcctctg cacggtgagc ccactcggaa
    11701 aatcagtggc gaggagtagg cgcttaatcg ctttcatttt cacaggacga tgtccgttct
    11761 cgatccttac ggtgttgacg gaacatcact gagggttcga acatacgcca tgacgtccaa
    11821 gatctctttg gctgacaact catacttcca cgcacccatt gctgtgttga ctcgcccctc
    11881 gtgaattgaa ttgtaaagcc tggagtcgag cttcgtttgc acttgtgggc tagtcaggtc
    11941 ggcggccggc gggttgaact tcatgacgcc gggtcccttt ccgtccagtc catggcaagc
    12001 cttacacagc gataaataga tacgcttgcc tgccgccacg tcggcactac ttgcttggat
    12061 gagtgacggc tgtcccatca gaaacactgg aagagcaatc gcggcaagca agggattcct
    12121 aagaaacatc ctgtcctctt tggctttgaa gtcaggtttg aagacggccg atcatcgtgt
    12181 tcgcttcctc gcgcaactgc ttgcgactta cacagcttcc gcactcagca gtaagagttc
    12241 acctttcata tcaggatgga tcgaacaacg aaacgtgtgc cgtccgggtc gttccatgat
    12301 gaaacgtatc gtggcatctc gctttcgatc taacatgacc cctccgagcc ctctcccata
    12361 gacaatcacg ccgtctttct caatccttgc ctgaataccc tcgaacatcg tggaaccaaa
    12421 atcatggcgc tcggtatctt cgttcacgat cttgatcacg gttggcatcc ccaaccgtag
    12481 gacttcttgt ttagtgataa aagagaagcc tttgatcacg acttcaacga cctgctcgga
    12541 ttgcgaatca acgggtggag ctgtgaagag ggcaagtggc cccaatacca ggaacatcaa
    12601 aatcgctgac tttgaacgtg tggacatgaa ggcctccctg tagctatgga cgcatatgat
    12661 ttgtgctgtt gctgacgatt accatacggc gctgtctcgt tgatcggatc ccgctacgag
    12721 ctgtattagg gtttgacggc cactttcaga acgccatcca gacgttcacc aaagattctg
    12781 tatccttcgt gaatattgtc cagagacacc cgatgagtga gcaacggcgt aaggtctatc
    12841 cgatgatgtc ggaccatctc gatgagacgt cgcatccgtt ccttgcctcc tggacacaga
    12901 gtcgtcacga tacgatgatc cccgagtccg gctgcaaatg cctcgtaggg gatctgaagt
    12961 tttcctgaat agacccctaa gcttgagaga gttcctcctg ggcggagaga gcgtagagca
    13021 ttctcgaatg tttcttgaag tcccaaagct tcgatggcca catcggctcc gccatcggta
    13081 agacgtttga cgtcttcaac gacgttcgtt tctcgctgat tgaggacgat atccgccccc
    13141 agacgtttgg ccatacgcag tctgctttca tcgccatcaa tcgcgatgat caaggatgca
    13201 cccatcagcc gggctcccac ggtcgcacac agtccaatcg gcccctgcgc aaaaatggcg
    13261 acactatcac cgattttcac gccgccagac tcggccccgc tgaaaccggt ggacgcgatg
    13321 tcggcgagca ggaccacttg ttcgtcggtg acgtcatccg gaatcttcgc gaggttggcc
    13381 tgcgcatacg gcactacgag ataatccgcc tgtgcgccgt tgatggtatt tccgaatcgc
    13441 caccccccga tggcttgata tccgctaccg tgaccacatt gtgcttgatg accggataga
    13501 catgcgttgc actggccgca aggtgtgatt gcaccgacca atacccgatc tccgacctga
    13561 tatccggcaa caccggggcc gagatcttca atgatcccga ccggttcgtg gcctatgatc
    13621 aatccagcgg gaaccggata ttccccacgc acaatgtgga gatctgtccc acaaatcgtg
    13681 gtcaaagtaa tccgaatgag cacctctcca tcggctacct ttggacgtgg aacctccatc
    13741 acctggaact tgtttttcgc ctggaacacg ttagcgcgca ttgtactcat gggcgcctcc
    13801 tgcagtgtga gatcccgcgc tctaaaattc agcaatatgc ataccggtgg ccgtaacgag
    13861 agagagacga cgcctatact actaagtatt gaatatgtac ggacaatgcc gtctcatgac
    13921 ttgaggacta agggagtttg gcccagcggt gggacacgaa gcaacactaa tcccttcggg
    13981 ctgccccgtt atgctacagc cagatacgat attggaacct cgctgcaaaa cgcatgttca
    14041 tcgaacctgt ggatccacat ctactagatt tgctttcctt gttttgatat ctgttgcggg
    14101 cctgccggag gtcgttatgc tggacattgc tcgaaatgtt cttgtcgttg atgatgatca
    14161 tgagatgcgg gcgttgcttc gggacgtgct cgagcagcac ggctataccg tgagtctcgc
    14221 ctccaatgga caggaagctc tcgtgaacct ccgtgaggca gagtatcccg tcgtgctcac
    14281 cgatctgagg atgaaaggga tgcaaggaat tgagctactc gcccagatca aacaatcgtg
    14341 gcccgaaacc aatgtaattc tcatgaccgc gttcggaagc gttgaaaccg ccattcaagc
    14401 catgaaacaa ggcgcctatg actatctgat gaagcccgtg aagaacgacg atctcttgcg
    14461 agccaccgaa cgcgggttcc gtgaagcact gctcagaagc gaaatcaacc ggctccggcg
    14521 agaagtagat aaggaataca gctttagtca aattcttgga aagagcaagg cgatgcgaga
    14581 ggtgttcgac ctgatccgcc gggtcgcaaa tagtccgacc aacatcctta tcacaggcga
    14641 gagcgggaca ggcaaggagc tggtggctaa ggctcttcac tacaatagtg agcggaagga
    14701 cgccccattc gttcccgtaa actgcgcagc gattcccgaa catttactgg aaagtgagct
    14761 gttcggtcac gttcgtggcg ccttcactga tgcaagagcg gacaagcgcg gtctgtttga
    14821 agaggcggag aaaggttcca ttttgctcga tgaaatcagt gagctgccac acatgttgca
    14881 ggcaaaactt ctccgagcca tccaagaaaa ggaaatcagg cgcgtggggt caactaagtc
    14941 aactgccgtt gatgtccgtg tgatcgcggc gacgaatttg aatcttgccg aagaagtgaa
    15001 agccaaacgg ttcagggagg acttgtacta ccgtctcaat gttattgagg tgaagcttcc
    15061 atcgttgcgt gaacggcgcg aggatattcc tctcctcgtc gattcgtttt tgaaaaagtt
    15121 cgggcaggcg cggggcaaag atgtcaaagg gatgagcgaa gccgcattgg ccatattgat
    15181 ggactatgct tggcccggga acgttcgaga attggaaaat gtcatcgagc gtgcggtcac
    15241 gctcagccgg gcagacagga ttctgccgga agatcttcca agcacgatac agggtgtgcg
    15301 cggtgaccgc cgcgttgttg acgagggagc ggagaagatg gttcctctac atgaagtgga
    15361 aaaagagtac atcaagaaaa tcctcgaaaa gatgggcgga aacaagtatc aggctgcaca
    15421 ggttctgggg atcgaccgca agacgttgta ccgtaagctc gcagagatcg aaggtacgct
    15481 tcctccggat gaatgaacgc cttaaatcgc cgtgctccta tgaaggtcgt caaggaagtg
    15541 gatatccgca gggtctcctg ggcgatcccg gttctgataa tcagcgtctt cgtttttgat
    15601 ctggccgctc cagtcggagt ggccgtctca gtgctctacg tgatcccctt gctatggacc
    15661 gttcatacag cgcgccagcg cgacgcattg tactattgtg ttgctgcaac ggcgctgctc
    15721 tggaccgccc tcttactaaa acccagtgct ttgctgttgg acttcagcgt gtttaaccgg
    15781 atgttgggaa cctttgtgct ctgtctcctc acctggggtt tgcttcactt gaagcggact
    15841 caacatgagt tactgcagtc tgaaatgcgc cggacgaaag cggtgcagga tttaatggcc
    15901 gagcgtgtgg agcgagccca tgctgaagga ctgatggagg tggctcaaga agcacgagct
    15961 tacgcagaga cggctgtcac cggagcggtg gccggccgcc agcgggccga agagcaatat
    16021 gtgatcagta aactcaggct cgaaggcatc atcgattccg ccatggacgc gatcatcacc
    16081 gtcgatcatg agcagaagat cgtcgtcttc aatcatgcag cggagcgcat gttcaaatgc
    16141 ccggcccaga atgcgattgg acagcccctc gacaggttca ttcccgctca cttacgggag
    16201 gcccatcggt cgcatgtgaa caggttcggg cagtctggcg tgacgaaccg aaaaatgggc
    16261 caactcggca ccgtgacagg tcttcgaacg gacggcgaag agttcccgat tgaagctgcg
    16321 atctcgcacg tcatggtcca aggaaccaag ttcttcactg ccatcctgcg cgacatcacc
    16381 gaacgtacgc aactggagga acagctgcga agaaccgaac ggattgccga actaggaacc
    16441 gtggcgtctg ggatggcgca cgaaattggt acgccgatga acgtcatcct ggggcgggcc
    16501 gaatatctgt tggaccgtgt caccgacgag ccggtaaaga aagggttgca aaccattgtt
    16561 gctcaggtcg agcggatcac gagagtcatg aatcaattgc tgtcgtttgc gcgtcgcaag
    16621 ccaccgcgac caggtcccct gcggctgcaa gaggtggtcg agaacagcct ggaaatgttt
    16681 catgaacgat tatccaacca tcaagtgcaa gtggatttgc aggtgaatgc cggctctccg
    16741 aacgtcctag gcgacagcga tcagatgagc caggtcatga tcaatctcat catgaacgcg
    16801 cttcacgcca tgccggatgg aggaatgctc cgcatcactc tcgggtcgga ggggggcatg
    16861 gcaaagctca cagtctcgga cacgggacac ggaattcccg acgacgttgt aagcaagatc
    16921 ttcgacccgt tctttacaac aaaagaattt ggaaagggga cgggccttgg cctgacggtg
    16981 gtcaagggaa tcatcgagga acatcatggg tcgatcgaag tggaaagtga gccgggaaaa
    17041 ggcacaacgt tcaccgtcct cctgccgaaa agctcgtaaa ctgctgcccc cggctgtggc
    17101 atggtgcgac agtgcaggga tatgactgta gtctttctcg ccaacccttt ggcttgaaat
    17161 cccctctgcc ggtcgatttc cagaataatg agcccttccc tcaccgattc ccgcggcatg
    17221 caccttgccc tttacgcagt tcaaagaaat ggattctccg gagatgccca caatggcggt
    17281 acataagaaa gcggtggtac ttgtcgtcga agacgatgca gatatgcgca atctactttg
    17341 tgacgcgttc tggagcgcag gatatcaatt acttgaggcc aaagacggcg atgaagcctt
    17401 cttggcggtt ttgcagtcgg tgccagacct catcctcact gatctccgca tgcctgccgg
    17461 gggagccgat tacattagcc ggttgcgcac agtggcaccc cactgtccca tcgtggtcat
    17521 gacggccttc ggcgaaccgg gattacaaac cgaagtcttg agcgcggggg cgacagcgta
    17581 ttttgacaaa cctgtccgaa tttctgatct caagctgaga attgaggcat tgctgacccg
    17641 atcaccagtg acggacgaag aacgagccta ggatggtcag atcttcatca ccggatgcgt
    17701 gttcgcagcg cgaccctgtt ttgacggctc gcattgagac aatcagtcaa ctcggcgaga
    17761 gcctctcgga tcggatcgcg atcatggacc gtcagttcaa catcctctat accaaccata
    17821 cgtatcgacc agcggaagcg tcaacccgat ccccttcaga gcagaccaag tgttatcaca
    17881 tctttgcacg tcggactgat cgatgcgccg tctgtccagc agcccaagcc ttcgagaacc
    17941 caagccgtga aggcagttcc tgtgcgatcg gttccgagac gagggcttgc ggtattcaac
    18001 gggcggttcc tctcttgggt gaaaatagac aggttgcgtc catgctcatc ttgttagaga
    18061 agcccgcacc atcgaacggt cttgccagaa tgcctgcctc cgaagaagcc gctcctccga
    18121 cggttcgaga acagttagac gagctgattg gccgcagtcc ggcgatgcag caactctttg
    18181 acatgatcac cctaatcgcc gacagtagcg ccacagtgct gatccaaggt gaaagcggca
    18241 caggaaaaga attggttgcc aaaagtattc accggttgag caatcggcga aggaagccct
    18301 ttgtcgtggt ggactgcgga tccctacccg aaaccctgct cgaaagcgaa ttgtttggac
    18361 acgtcaaagg agcctttacc ggcgccgtgg ccaacaagcg agggttgttc gaagaggcgg
    18421 acggtgggac gatcttgctc gacgaaatag ccaacaccag ccagaccttt caagccaaac
    18481 tgctccgcgt ccttcaagag ggtgaaatca ggcgcgttgg aagcaatcaa ccgatcaaga
    18541 tcgatgcacg cgtgatctcc gccacgaaca aaaatctcac cgagttggtc agtgtgaagc
    18601 agtttaggca ggatctctat taccgcttgg ccgtccttcc tctatatctc ccaccgttgc
    18661 gtgagcgcaa ggaggacatc gcgctattgg tcgaacgctt tcttatgcag tcctgcgcga
    18721 agcatggcca gccaggtcgc catgtctccg acgagatgat gtgcaggctt acagaatcgg
    18781 cctggcctgg aaatgtcagg gagttaaagc actacattga acgggccgtc atcaccacca
    18841 taggaccggc gctgacgtgc caggacatcg tcgccaagag aacggactca ccagcgctgg
    18901 acctccgttc tgtgtcgcgt ggtgccatga ggcaggtgga acgggcgcaa attgtacagg
    18961 ctcttgccct cactcatggg aatcgcttga aagccgccaa atacctcaag attagccggg
    19021 cgagcctcta caataagatc cggtcctact cgatccgata acacccgctt ctgcccctct
    19081 tttccccatt ttctgcgacc ggcatgtctc agcatttgga cattcgaaac cattgaaaca
    19141 atgtgaacgg tcttcgccgg cgcatgtcag tgtctagttc tgtagaaagt cgccgcactc
    19201 aggcgttgtg tcacggcggc tcgctttccc gcaaactgcc gcgccatcaa agcatttcgg
    19261 ctttttaccc ctgccacgca tcgaggcacg tctgttgcgc ttatgagaag aacgctggtg
    19321 aaggttcggg tgattaggac cggcctccga atgacccgct tcttcgagag aaggagccct
    19381 tcaccagctc tattcaggag gccgtgagag gtctttttga taacaaatcg cgagacggat
    19441 atggactttc acacaaccgc tcacattggc ggaaacaaga aggggaggcc gagtatgaag
    19501 tctttcatag agcaatcaca aggcaagcga cgatggggaa gcctggtgct gatgctcccc
    19561 actgtcacac tgacgtgtgg cctcacggca acaccatggc tattcgcggc aagtacggga
    19621 gaagcatccc atgagggcca tgcgacggcc gtcgccatgc ccggctggac gcaacaactc
    19681 aagggccaga cggtggtgga gaatgccatc gaaggccggg ccggcaatgc tgaaaagatg
    19741 gaaatgcagc atcatcggct gatgcagaag ctggagcaac aggcccaaga agacgccaag
    19801 gctcagcaaa cttcaggcgc attcaacaac atgtcgatga tgcatcagta catgggccag
    19861 gacggcagca gtttcctcct catgactgat tccagcaaag gagaaccggt gtccgcattg
    19921 ggcggcaagt gtccgtccaa tgttccggtg cggatgtacg atgtctcgat gatcaacatt
    19981 gagatcacct tgaatcgctg gctcgattac tatcccggtt atatgtacgt cttgactgaa
    20041 gacgtcgaga aggtccgcgc agaagaaacc agaaataaag acgcgcgtga caaagaggga
    20101 ttcgatcccg gctccgtcac gaccggcctg cagggggaca tgattcagcc gttggtgctt
    20161 cgagccaatc agggcgactg cgtgaggatg acgctccgca atcagatgga aagcgaggac
    20221 ggcagcctct tcatccaagc ttccagcatg attgtgagtg caacgggcaa gccagccacg
    20281 accaccaatc cggaatcgat catttcgccc ggcaaattgc aggaattcga atggtatatc
    20341 cacccgcaga tgcaggaagg cgtccggcaa tttcattcct atagccatga ccgcgaatta
    20401 acagtgctcg gtctcttcgg cgcgttcgtt gtagaaccga aggggtcgaa gtatctggat
    20461 tccatcggca ccgggtcgga taagcaagtc aagacgggct ggcaggtgaa cattgataat
    20521 ggatctggac cagactttcg cgaattcgtg ctgttctacc acgaggttgg cgatgaagcg
    20581 ttcaggccgt tgaacaagaa gggcgacttc ctgccgcagc gcgatccgct gacggatgcc
    20641 tatcggccag gcggccgggc gatcaattat cgcagcgagc cgttcggcat cgatcagatg
    20701 catctccaac atgagtattt cggctttgaa gacgaatcca tggcctacag cgcctacacc
    20761 ttcggcgata cgccgaccac catcgctcga ggctatttgg gagaccccgt gaagtggcgg
    20821 ctggttcatg gcggatcgga ggtgtttcac tcccatcatc cgcacagcgg atcgatccgc
    20881 tggcagcgca gtccgggtac cgagaccaac aacatgtggg cgatggggca ggacggtccg
    20941 gtgaagtatc cggtcgtgcg gaccaagtct gaccgcgtgg atgttgaagt gatcggtcct
    21001 tcggaggcct tggatcttga accggaatgc gggggaggcg gctgccagca tctggctggc
    21061 gagttcctgt accactgcca cgtggcgcac cactatgtgg cgggaatgtg gggctacggg
    21121 cgattttaca acacgttgca agtcggcgcg gcgcataccg atacgatgcc ggatctgcaa
    21181 gagctgcctg atcgcaaagg ccggatgaag gtcggcgtaa cgtccgataa gttgatcggc
    21241 acgaccgtcg actggttcgg caagtccttc aaaattgtgg acaaggggca gaagacgaat
    21301 tggaaatcca atccggtcat tgtgaacatc aaggattggg tcgaaatgtt cgtgcctgct
    21361 caaggcaagc cgggccacac ggacaaggag aaggatcaga tcctcgccta tgactcgacg
    21421 gtaagggatt ggaagtggga tggcaatatc gcccggggcg agcgcgaaag caccgcacag
    21481 aatccgaagt atccatgggc cgccaagtgg gacgacagca ccagacctgc cattctcttt
    21541 gatccgacca cgggcaagat ggcctggccg ttattcaagc cgcacttcgg caagcgggtg
    21601 cccttctcgg ccaatcacag cggcgcgccc tggcttgagc ccattcacca ggatgcgaac
    21661 ggcgaacgga ccagcgagcc ggccaagccc ggtgagcaag ggcgttggag tctctgcccg
    21721 gacaatgcca atcaaaagtt ttacaacctt cacttcctgc ggatgccgat caccttggcg
    21781 aaacagcagg ggaaagaacc gcccatcgtc gacaaggacg ggctgattta cgtgctccat
    21841 gaagaagagc gtctgacgag aggcaatgac gatctcaagc tcccggcggt catccgcgcc
    21901 aatgtctacg attgtgtcga cttggttctg accagcgaat gggacgacga cgattacacc
    21961 aatttccagt cgtccaagat caacatccat ccccacttct tccaattcga cacagggaac
    22021 tcggatggcg tgatttcagg gttcgaatac gaaatgtccg tgcgtccgtt caccatgtgg
    22081 ggcaaggcca agaaacacgg cttaccagcg ccgatggtag ccaagatcgt cggcggagct
    22141 aaggcgggag ccagctccat caagattcaa ctggcccccg gtgccacgcc attccatgtg
    22201 aacaccgaag tcatggtcgg cgtggactgt ttggaaaagg gaaacgatcc cacggcctcc
    22261 ctgccacggg ataagagctg ctccgaagtc gcccgcatca aggacatcaa gggcgatcag
    22321 gtcacattct tcaagccgct caagcacaac catccggcca gcgacctcgt gtcgccggag
    22381 ttcgtgcgct accgttggtg ggtggacgtg gatatgggga cagtgttctg gcatgaccac
    22441 gcgtttggcg cgacgacctg gccgcacggc gggttcggcg tgacgatcgt ggagccgttc
    22501 gggtctacgt accataatcc aaaggacggc aagctggtgt atagcggtcc ggttgcagat
    22561 attcactcga acgagccgat cggagccggc gtcagcggaa gcttccgcga actgatggtg
    22621 tcgatccacg atacggtgcc gcacaccgtc aacgtcatcg aggccggtaa tcctcccgga
    22681 cagcctgtcg aggtggcgct ggaagcggga aagacggtgt cgttccagat gcccgaaaag
    22741 atcttgaacg cgcccaacaa gtacatcaac ggcgggacac atacgacggg cagcgggttc
    22801 aatttccgtg cggaaccctt cgctcagcgg ctgtcgaata atccggatac atcgaagttg
    22861 ttcagcagcg caatccatgg tgatcccgat accccgttgt tacgggcgta taccggtgac
    22921 acgatggtct ttcggctgct gcatcagttg atgaacgagt cgcatgtgtg gacgatcgcc
    22981 ggccatacgt tcctcacgga gcgctatgcg gcggatgcca acaggaagaa ctccattcac
    23041 gtcgggatcg ccgagcggta cgatctcgtg acgaaggcgg gtgggttcca gggcatgccg
    23101 ggcgactaca tccacttcaa cggtcgaagc tcacacttcg ccgaaggcgg atggggcatc
    23161 gtgcgcgtgc tcgataagga aacgtccgac ctcaagccac tgccgaaggg cacgaatccg
    23221 ctgggcattc caacgactcc cagtggagtc tgtcccgccg atgcgccggt caagagcttc
    23281 accgtcgtgg cgttagatcg cccgctgaag ctgcatccga aagcgcctga agcaatcgaa
    23341 gttgatttcg agcgcaagat cgagctgacc gtgccggagg gcaagatctt cgcgcttgaa
    23401 gaggaagcga cgacggtggc gggcaacgtc atgccgaatc cgctgacact gcgcgcgaac
    23461 ctgggcgact gcatcaaagt caatctcaag aataagatga aggccagccg ggcatcgttc
    23521 tttgcgcccg gattagcctt tgatcccaaa gacagccagg gcttgaacgt cggcaacaac
    23581 ggcggcgatc agaccatcgg acccggtgag agccggaact acacctacta cgcgcatccg
    23641 tcgaacaagg aaacgacgtc gttggtgtgg gacggcggca acatcgtcgt caatccgcgc
    23701 aacggattgt atggcgctat tatcatcggc ccgaaaggat cgcaatatcg tgatccggtg
    23761 actggggccg atctctcgca gaaaaatgcc tggagagccg atgtcgtcgt cgataccagt
    23821 ttgccggaaa acgtcggcaa gcggaattat cgagacgtgg cattgttctt ccaggacgaa
    23881 gacaacatca tcgggacctc gttcatgcct tatgtgcaga acgtggctgg cctgacgtcg
    23941 gtgaattatc gcgccgaacc atacaagtat cgtgaagagc agggctgctc gctggggaag
    24001 attttccaac cctgcgtagt ggacaagccc gaggatccgg ccacgccgct gatcgaggcc
    24061 catgccggcg atccgatccg cattcacgtc atcggggcga acagcgagca aaacggaatg
    24121 ttcggcgttg agggccacga gtggccaatt gagccctaca tgccgggcgc cgacatgatc
    24181 agtgtggtgg agtatgccgg ttcggaagtg ctcgacgtct tcatccaggg cggggcggga
    24241 ggtccgtatc gccagactgg cgattttgtg tggtcgaaca atcggctgcc gtacgctcag
    24301 tcaggacagt ggggctacct gcgtgtgttg ccgacgggcg attcacggat ccaaccgttg
    24361 ggtgcagcca gcgcgggcgc caagcgggcg gacgcgcggc cggcgccgcg ggcgactcca
    24421 accgccatga aataggcacg ttggtcttgg tgtaaggtct gacagggagc tgccgccaac
    24481 cgggagctcc cctgttcctt ctgttaggca tgccggcaga gcgggcagaa gtgaatgctt
    24541 ccgattcgac gaatcattct agactacggt cgcagttgcg aacgtcttct gtcgagccgg
    24601 ttactcgagc ttccactaac gactgatgag atgcaactca tccgatatta cctgagagat
    24661 ggaacacaag ttcaattcag acatagccag ccatgaatct aaacgctcag aggactcacg
    24721 tcatggaaaa gaacgagatc aataagtggc gtagatggga gatggcggtc aacgtgctgt
    24781 tggtcgcggt ggcgctggtg accattgcca atgccgcttg gggcctgcag actccggacg
    24841 tcaccgcatg ggcaccgaaa ggcacgccgc cggttgggtc ttcccacgtc ctcatcgccg
    24901 ctcgacgcga cgaaatgccg gcgaaccgga cgccgtgacg tcttacactt cgtaggccgt
    24961 gttggcctca ggcaccctca catcgacatg cggatgctct gccgcaatcg cagcagccaa
    25021 tgaaaacgcc tgccggtgct ctccatgcac gatgaaaatc tgtgaaggaa gggggttgat
    25081 cgcgcgaacg tagcccagga gatcttcgcg atcagcatgt gcggataagc cgttaaaatg
    25141 gaccaccctg gcacgtcgag gcacggggac tccgaaaatc ggcacaggat cccatccttc
    25201 tatcaacttg cggcccagtg tgtgctctgc ttgaaatccg acaatggcta cgacgttggt
    25261 ttcatcctga atggcatgcc gtaagtggtg cagaacgcgg ccaccttcac acatgccaga
    25321 ggcggcaata atcacgcacg gtcccctcat ggtgttcagg ttttggcttt catggacgga
    25381 tgacacatag cgaatatact tcgcgatgaa aggatctccc tcggtcgtga gaatccgata
    25441 agtttcttca tcgtagcatt cgggatgccg acggaagact tcagtcgcac gcaatgccaa
    25501 cggcgaatct atatagatgg gcaccggcgg gacccggcct tctttcacca tatcctttac
    25561 ccacatgacg aggtcttggg tgcggcccac agcaaacgcc ggaacgataa ttttgcttcg
    25621 ggtagcgatg gcatggtgga tgagggcact ggctttttgt ttggcctcat cggccgtttc
    25681 gctgtgcagg cggtcgccat aggtagattc aatgatcaaa acgtcgcagg aaggcggggg
    25741 ttgcggatct cgtaatattg gcatttggcg acggcccaaa tctccggaaa acagaatcgt
    25801 tttcgtcgcg ccagcctcca tgatccgaag agagatggcc gctgatccaa gaatgtgtcc
    25861 ggcatcaagg aacgtcgctt tcataccagg cagcggcgtc acgacatcgc cgtaacggag
    25921 accgacgaat tgtcgtttaa tcgtttggac atcgtcactt gtgtagaacg gctgccggca
    25981 cattccctca gtgcggcgtt ccttgcgatt gacatagaca caatcgctct cctgaatgtg
    26041 cgccgagtcg gtcaacataa tttcgctcaa ctctgccgtc gccctcgtcg cgaagacctt
    26101 tcctgaaaag ccatacttgg ccaaagccgg cagggctccc gaatgatcaa tgtgggcatg
    26161 agagaggaag acggcgtcaa tttccttggg atcaaaaccg agatagcgat tgcggcgctc
    26221 ggtctcctcg cgccgtccct gaaaaagtcc gcaatccaag agcacacgat tcccatcggt
    26281 ctcaagcaag tgcctgcttc cagtgaccga tcggacagcg ccgtgaaagg tgagtttcat
    26341 tggaaccacc gcgggggcgg aggccggcgt cgaaacgtct gctgggtgcg ggcaatgagt
    26401 ttccaggctt cctcggccgt gtcggcgtac tggaatagct ttcgatcacg agcgctgatg
    26461 aagccgttgt cttccagcag ttgccagtta atcaaccggc tccaatactc ccggccgacc
    26521 agaataacgg tgatgccgcg gactttcttg gtttgcagta atgtcaacgc gtcgaagagc
    26581 tcatcgcagg tcccaaatcc tccaggaaag gcaacgagcg cctttgcccg caacaaaaaa
    26641 tgcatcttgc gcagcgcaaa atatcggaac tgaaaacata gtgcgggggt gatgtaggga
    26701 ttcggttgct gttcgtgtgg aagcgaaata ttcaggccaa tagatttgcc tttgacgtct
    26761 gcggctcctc gattggctgc ttccataatg cccgggccgc cgccggtcac caccacataa
    26821 tcgcagccac gctttgactg gtatttgctg gtaaccagcc gtccgaaagc ccgagcttcg
    26881 ttatagtatt tagacatagc gacttgccgt ttcgccatgg caactgaccg ctgacgggac
    26941 acatcgtgcg gtttgcctgc taaacgttta tttgcttcgt cgagacgccg gttgctgact
    27001 gcaggctctg gaattcttgc gcttccgaac accacgatgg tcgaacggat attctcccga
    27061 cgttggatca attccgtctt cagcaactct aattgaagac ggatcgggcg aacctcatca
    27121 ccgttgagaa atgcgagatc ttggtcggct cg
//
LOCUS       DASTQY010000329         5506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_91257, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000329 DASTQY010000000
VERSION     DASTQY010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91257"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VFR82_10810"
     CDS             <1..996
                     /locus_tag="VFR82_10810"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU4502132.1"
                     /translation="QLFFQAQQLFELAGKRGYRIESAGFGAQSAEAAFEDAVRQLGFT
                     VKDSYYRVQLAQRRLALAEENRDRFSRILEINTIRFKKGYIAEVDLIRIRLQMVDFQS
                     QVIQSLQEAESARGDLRQLLRLSPATTVELTTDLEFRRIDPEIAKLRLAAIDVRPDLR
                     SKRFTYSQREADLRLAQAYRVPDVTVGAGYAIQGSLGPDNPQQWALNLGLPVPLFNRN
                     QGGIRQAEVAVQTAGADLNKAVNVVENEVEVAYRNLLQSRRLVEAYVGGVLEDARATF
                     TIVERAYERGGATILDLLDAARTSRTIQQNYIEALFNYQRNVLQLESAVGQEIAS"
     gene            993..2216
                     /locus_tag="VFR82_10815"
     CDS             993..2216
                     /locus_tag="VFR82_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU4502133.1"
                     /translation="MTVQGRTESQVAMDQPLEEARAGLLSPRGAHVVCVLAITALVGC
                     GKGDQPANSDAATPPHTKNAPVLHESVSRVETGIVDYSGSQQDLALSGKMAYGEDRYS
                     KISSPLQGRVVEVRAHLGDRVKAGAILLVVDSPDIAQAYSEFVKEDSDLQYATRSYEL
                     AKDLYATKALPLKDLKQAENELVKARAEFRRAKERLLSLRIPAEEVNKPLDKQQITSR
                     FEMKSPLTGIVVERMVTPGQSVGGDANQVLFTVADLDMLQVVADVYERDLALVREGQF
                     AKVKVEAYPDVDFPATVAAVGDVVDPASRTIKLRAWVNNPDHRLKPEMFARLHIQVGD
                     STRILVVPKEAVLESDGKQFVFVVEEANRYIKREIKAANFTPDQMRVLDGLTPGQRIV
                     TKGAVLIKGQEVKGG"
     gene            2274..5342
                     /locus_tag="VFR82_10820"
     CDS             2274..5342
                     /locus_tag="VFR82_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEU4502134.1"
                     /translation="MIARLVEISLVQRILLCALGLTLLFGGLYSFHLLDIIAYPDPSP
                     PMVELITQNPGWSAEEIERQITIPMEVALTGMPGLTDIRSLSIFGLSDIKVYFDFGTD
                     IFRDRQEVLNRLASVDFPLGVQPVLSPWWAIAEIYRYELTGEPGVNLTELKTIQDWQV
                     RRAFRRIPGVIDVTAFGGTTKEFHVDIDPGRLISYGVSLSQVMTALQNSNANVGGNYL
                     TLGAQNYNVRGVGLINSIEDIENVMVAQKDGTPIYVKTLGQVKVGSRIRLGKVGIDER
                     DDVVEGVVLLQRGYKALPVLDKVREKMDELNEWKLPAGVKIRTFYDRTTLIHTTVETV
                     TDIMISGMVLVFVLLYAFLGNLRAAGIVALTIPLSLLFTFAMMVLVGQSANLISLGSI
                     DFGIIVDATLIMVESVFFHLAHAKTPGLTVHQQIVRAARQVGQPIFYSTAIIVVAFIP
                     LFTMTGVPGKIFAPMSITYGFALLGALLMAFTLAPVLCSFLLTGPITEHDTIVVKPLR
                     KLYQRTLEWALDHRKVVVALAASLLIVALVALQFLGGEFMPALEEGNLWVRATMPVDI
                     SFDQAARLTSDIRAMFRESPEVTTVVSQLGRPDDGTDPTSFFNAEFLANLKPQAHWRR
                     GLGKDDLVEEIESRLKTIPGVIFNFSQVIQDNVEEAMSGVKGENSIKLFGADLKTMET
                     LAGDIEKTMHQVRGVKDLGIFRLLGQPNLLIKVDRQASARYGLQVADVNAVVQAAIGG
                     QAVTQVYEGERLFDLVVRFLPEYRQDVEAISNILVSTPDGARIPLKQVATITSQTGAF
                     IIYRENNERYIPIKFSVRDRDLESTVEEAQALLAKRITLPERYRMEWAGQYDQLKDEQ
                     RRLAKIVPLSLLLILFLLYITFDSLKNALLVLSAVPFALIGGVIALVATHTNFSISAA
                     VGIISTLGVAILGGVLLISRIEDGRMAGLNLREAIIQAAAVQMRPILMATIGAAIGLL
                     PAAVATGIGSQAQKPLARVVVGGMLTAAFLILVVLPVLYEMVHRRTDQDE"
BASE COUNT         1147 a         1518 c         1634 g         1207 t
ORIGIN      
        1 cagctcttct ttcaagcgca acagttgttt gaattagccg gtaaacgagg gtatcgcatc
       61 gagagtgccg ggttcggagc gcagtccgcc gaagccgcgt tcgaggatgc ggtccgccaa
      121 ttgggcttca cagtaaaaga cagttactac cgggttcagt tggctcagcg gcggctcgcc
      181 ctggctgaag aaaaccgtga tcggttttcc cgcattctcg agatcaatac gatccgcttc
      241 aagaagggct atatcgccga agtggatttg attcggatca ggttgcagat ggttgatttc
      301 caatcacagg tgatccaatc cttgcaggag gcggaatcgg ctcgcggcga tctgcgacaa
      361 ctcttgcgcc tctctccggc gacgaccgtc gagttgacga ccgatttgga attccgtcgc
      421 atcgatccgg agattgcgaa actgcggctc gcggcgatcg atgtgcgtcc cgacttgcgt
      481 tcaaagcgct ttacctactc ccaacgggaa gccgatctcc ggctggccca agcctaccgt
      541 gtgccggatg tgacggtggg agccggctac gccattcaag gctcgctcgg tcctgataat
      601 ccgcagcaat gggcgctcaa cctcggattg cccgtgccgt tgttcaaccg aaatcagggc
      661 gggatccgac aggccgaagt cgcggtccag acggcaggag ccgacttgaa taaggctgtg
      721 aacgtggttg agaatgaagt ggaagtcgct tacaggaatc tactgcagag tcggcggctg
      781 gtcgaagcat acgtaggtgg tgtgttggag gatgcgcgcg ccaccttcac gatcgtggaa
      841 cgggcgtatg aacggggcgg tgccaccatt ctcgatctgc tcgatgccgc gcggacgtcg
      901 cggactattc aacaaaatta tatcgaagct ttgttcaatt atcagcggaa cgtgcttcag
      961 ctggaatcgg cggtcggcca agagattgcg tcatgactgt acaaggtagg actgagtctc
     1021 aggtcgcgat ggatcaacct ctggaagagg cgcgtgccgg actcctttct cccaggggcg
     1081 cacacgtcgt ctgcgtcctg gcgatcaccg ctctggttgg ctgcggaaag ggagatcagc
     1141 cggccaactc agacgccgcc actcctccgc acacgaaaaa cgcccccgtc ctccacgagt
     1201 cggtctcgcg cgtggaaacc ggcatcgtgg attacagcgg atcgcagcag gatcttgcct
     1261 tatccggcaa gatggcttat ggggaagacc gttattcgaa aatttcctcg ccgctgcagg
     1321 gtcgggtggt cgaggtccgt gcccatctcg gcgatcgggt caaggccgga gcgattcttc
     1381 tcgtggtcga cagtccggac atcgcgcagg cctactccga attcgtaaag gaagactctg
     1441 atctacagta tgccacccgt tcgtacgagc tggcaaaaga tctgtacgcg actaaggcgc
     1501 ttccgctcaa ggatctgaag caggctgaaa atgaattggt gaaggccagg gctgagttcc
     1561 gccgggcgaa ggaacgcttg ttgtccctgc gtattccggc cgaagaagtg aataagcctc
     1621 tcgacaaaca gcagatcacc tcccgttttg agatgaagag cccgttgacc gggatcgtcg
     1681 tcgaacgcat ggtgacgccg ggccagtcgg ttggagggga tgccaatcag gtgctgttta
     1741 ccgttgccga tcttgacatg ctgcaagtgg tggctgatgt ctatgaacgc gatctggcct
     1801 tagtcaggga agggcagttt gccaaagtca aggtcgaggc gtaccccgat gtcgactttc
     1861 ccgcgactgt ggcggcggtt ggggacgtgg tcgacccggc aagccgcacg atcaagctac
     1921 gggcctgggt gaacaatccg gaccatcgtt tgaagccgga aatgtttgcc cggttgcaca
     1981 ttcaggttgg cgactcgacg cgcatccttg tggtaccgaa agaagccgtc ttggagtccg
     2041 acggcaaaca atttgtcttc gtcgttgagg aggctaaccg ctacatcaag cgggagatca
     2101 aggccgccaa ctttacgccg gaccagatgc gcgtcctcga cggattgacg cctggccagc
     2161 ggatcgtgac caagggcgcc gtattgatca aaggccaaga agtcaaaggc ggctgacgga
     2221 gtgactcctt catctccttc ctatcgctgt agtcccccgg atccgatgag ttgatgatcg
     2281 cgcgtctcgt cgagatatcc ctcgtccagc gtattttgct ctgcgcctta gggctgacgc
     2341 tcctgtttgg cgggctctat tccttccatt tactcgacat cattgcctac cccgatccat
     2401 cgccgccaat ggtggagtta atcacccaga accccggatg gtcggcggag gaaattgaac
     2461 ggcaaattac gattccgatg gaagtggcgc ttaccggcat gcccggtctg accgacatcc
     2521 gctcgctgtc catcttcggc ttgagcgaca tcaaggtcta cttcgacttt ggcaccgata
     2581 tcttccgcga ccgtcaagaa gtgctcaacc gccttgcgtc tgtcgacttt cctctcggcg
     2641 ttcaaccggt gctttcaccc tggtgggcca tcgcggaaat ctatcggtat gagctcacgg
     2701 gcgaaccggg cgtgaacctg accgaactca agaccatcca ggattggcag gtgcggcgtg
     2761 cgttccggcg catccccggt gtcattgacg tcaccgcctt cggcggtacg acgaaagaat
     2821 tccacgtgga catcgacccg gggagattga tcagctacgg ggtcagcctt tcccaagtta
     2881 tgactgcctt acagaacagc aatgcaaacg tcggagggaa ctatctcacg ttgggagccc
     2941 agaactacaa cgtgcggggg gtcggtctga tcaatagcat cgaagacatt gaaaacgtga
     3001 tggtggcgca aaaggatggc acgcccattt atgtcaaaac gctcggccag gtcaaagtgg
     3061 gcagccgtat ccgactggga aaagtcggca tcgacgagcg ggatgacgtc gtggagggcg
     3121 tcgtgctgct ccaacgcggg tacaaggccc tcccggtgct cgacaaagtc cgcgagaaaa
     3181 tggacgagct caacgagtgg aaattacctg ccggggtcaa gatccggacg ttttatgatc
     3241 gaacgacgct gatccatacg accgtcgaaa cagtcacgga catcatgatc agcgggatgg
     3301 tgttggtctt cgtgctgctg tatgcgtttt taggcaatct tcgggccgcc ggcattgtcg
     3361 cgctgaccat cccgctgtcg ctcctcttta cctttgcaat gatggtgctg gtgggccagt
     3421 ccgcgaacct gatttccctt ggctcgattg atttcggcat catcgtggat gcgacactga
     3481 ttatggtaga gagcgtcttc ttccatttgg cgcatgcgaa aacaccaggc ctgaccgtgc
     3541 accagcaaat cgtgcgcgct gcacgccagg tcggccagcc catattctat tcgacggcca
     3601 tcattgtggt ggccttcatt ccgctgttca cgatgaccgg ggtgccggga aaaatttttg
     3661 cccccatgtc cattacctat gggtttgcct tgctcggcgc gctgctgatg gcctttacgc
     3721 ttgccccggt gctgtgctca ttcctcttga cgggtccgat tacagagcac gatacgatcg
     3781 tcgtcaagcc gctgaggaag ctgtatcaac gcacgctcga gtgggcgctc gatcaccgga
     3841 aagtcgtagt tgccctggcc gcctccctcc tcattgtggc cctcgtggcg ctgcagtttc
     3901 ttggcggtga atttatgccc gcgttagagg aaggtaatct ctgggtgcgc gccaccatgc
     3961 cggtcgatat ctcgttcgac caggcggcgc ggctcacgag cgacatcagg gccatgttcc
     4021 gggaatctcc ggaagtgacg acggtcgtct ctcagcttgg acggcccgat gacggcaccg
     4081 accccacgag ttttttcaat gctgaattcc ttgccaacct gaagcctcag gctcactggc
     4141 gcagagggct cggcaaggat gacctggttg aagagatcga gagccgactt aagacgatcc
     4201 caggggtcat cttcaatttt tcgcaggtca ttcaggacaa cgtcgaagag gcgatgtccg
     4261 gcgtcaaagg agaaaactcg atcaagttat tcggggcgga tctaaaaacg atggaaacgc
     4321 tggcggggga catcgaaaag accatgcatc aggttcgagg tgtgaaagac ttgggaattt
     4381 tccgtctgct cggccaaccc aatttattga tcaaggtcga tcggcaggcg agcgcccgct
     4441 acggcttgca ggtggccgat gtcaacgccg ttgtacaggc ggcaattgga ggtcaagccg
     4501 tgacgcaggt gtacgaaggc gagcgcttgt ttgatctggt cgttcggttt ctccctgaat
     4561 atcggcagga cgtcgaggcc atcagcaaca ttttggtgag tacaccggat ggcgcccgca
     4621 ttcccttaaa gcaggtcgcc acgatcacca gtcagaccgg agccttcatc atttatcgcg
     4681 aaaacaatga gcggtatatc cccatcaaat tcagcgttcg agatcgagat ctggaaagta
     4741 ccgtcgagga agcgcaggcg ctgctcgcta agcgcattac actgcctgaa cggtatcgca
     4801 tggagtgggc cggccaatac gaccaattga aggatgagca aaggcggttg gccaaaatcg
     4861 tgccgctgag cctcctgttg atcctcttct tgctctacat taccttcgac tcgctcaaga
     4921 atgccttgtt ggtcctttcg gcggtaccct tcgcgttgat cggaggcgtg atcgcgctgg
     4981 tcgccactca tacgaacttc agcatctcgg cggcggtggg tatcatttcc accttggggg
     5041 tcgcgatcct gggcggcgtc ttactgatct ctcgcatcga agacggccgt atggcaggac
     5101 tgaacctccg cgaagcgatc atccaggccg ctgccgtaca aatgagaccg attttaatgg
     5161 cgacgatcgg cgcggcgatc ggactgctgc ccgctgccgt ggcgacgggt atcggctctc
     5221 aggcccaaaa gcccctagcc agggtggtcg tcggtggaat gctcacagcc gccttcttga
     5281 ttctggtggt gttgccggtg ttatatgaaa tggtgcatcg gcgaacggac caagacgaat
     5341 aacgaaagag gacgatcgtg tatatgagat ggaaatggat gttggtcaca gtcgtcatga
     5401 tgggtttggg cctcagtccg ggcggacctg agttgtttgc acaatcaggc ctgcgcggcc
     5461 tgaccgttcc cgtgcaaatt ccctatgaaa tgccgcccca gtttca
//
LOCUS       DASTQY010000330         7297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_26900, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000330 DASTQY010000000
VERSION     DASTQY010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26900"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..853)
                     /locus_tag="VFR82_10825"
     CDS             complement(281..853)
                     /locus_tag="VFR82_10825"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEU4502135.1"
                     /translation="MQIPLKAQLGKGIFLVAAPNLRDPNFRQTVVLLCEHGAEGALGV
                     VVNRPTAMSVSEALPQMPVLEGQRHVLFSGGPVQPNQVMLLYRLDQLPDNSHHVFDGV
                     CLGGDMELVDKILTTDHGKEAFRAYVGYSGWGPGQLESEMKIGSWITVPADPATVFEK
                     DPTRVWSEILATLGEEFRVYADMPFDPSLN"
     gene            complement(919..1707)
                     /locus_tag="VFR82_10830"
     CDS             complement(919..1707)
                     /locus_tag="VFR82_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEU4502136.1"
                     /translation="MRVGYYQNNPEFGKVAENLDRIAAKLEDAEADLLVLPELCASGY
                     QFVSMDEVRALAEPIPAGQTTKRLLDLAKRRQMVIVAGLPEQAGTAYYNSAVVVAPQG
                     FIGSYRKTHLFFEETLYFTPGDTGFHVWDVGPAKIGVMICFDWYYPEAARVLALKGAD
                     IICHPSNLVLPNCPDSMPVRCLENRVFAVTCNRTGSEAREGKDRLTYIGNSEVVSPRG
                     AILQRAPRDQEALCVVDIDPAEARNKSLTPYNDLLRDRRETFYR"
     gene            complement(1875..2210)
                     /gene="smbP"
                     /locus_tag="VFR82_10835"
     CDS             complement(1875..2210)
                     /gene="smbP"
                     /locus_tag="VFR82_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HEU4502137.1"
                     /translation="MTMSIRRGAAAMAAVALLGLPLVAWADNKHVSEAVEHAKGAVNH
                     GKQGHADAVAQHAEEALKHAQAAGVKNPHLDEGIKHLKEAITHGKAGHADQATEHAEG
                     AVTHLSEVK"
     gene            complement(2302..3180)
                     /locus_tag="VFR82_10840"
     CDS             complement(2302..3180)
                     /locus_tag="VFR82_10840"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU4502138.1"
                     /translation="MNHPLLIDTETLHKQLGKPKLVVIDVRGNAAYEYGGHIPGAVHT
                     TWHHYSDPNAVAKGLLDPDMNRMEQKMRALGLNDDSEVVIYSNPFDNWGDEGRMFWML
                     EYFGHKNMRILDGGWVKWVTEQRPFEHGRVSPKPGNFTVRQATHTIIGKDELRAMVKH
                     PPSDMKILDARSLEEYLGKEVSGIPRPGHIPSAIHLSWNGFLNTNATVKDLAAIKAAL
                     EEKGLEPQHDIVCYCTGGVRSSWLYFVLKLAGFQKVRNYPGSWWEWSRDFACPVEKDF
                     QGLQKVLGIEAPARPS"
     gene            complement(3190..4482)
                     /locus_tag="VFR82_10845"
     CDS             complement(3190..4482)
                     /locus_tag="VFR82_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEU4502139.1"
                     /translation="MRRWKFMVIGLLVVFGATYLYYTEIKPVVIFGLRSDYAHAIPFQ
                     KVPDGLTSLKAESCGQCHREIYDEWKSSIHARAYEDPYFQAYWKKDKNVWICLNCHSP
                     LENQQPTVITDIPRGRVEKAVQEPNPHYDAEYQKESVTCAACHVRDGIIYGPFEDSAA
                     PHPTQFDPNFRTAQLCYRCHNVVSGPAQFYRVGPCGTYAEYEGKFFMQERGFICQSCH
                     MPEIDRPVATNSPIRRGRQHLWRGGHDPDMIKRAVAVEVKADPPSPKPGDRVAFTLTL
                     VNAGAGHKIPTGDPDRFFTVEFTVEDTLGHVLKEETSTMGRWIMWQPAILELYDNRLL
                     PLASREYQFAYQLPDSAEDLKVKTRVQYHIVTDKQHEMLQRRYGLTGNDPYRFVIYER
                     EFPLTDKLKAALDANVQPSVAGSSQHGSSCAVDTVGRG"
     gene            complement(4479..5420)
                     /locus_tag="VFR82_10850"
     CDS             complement(4479..5420)
                     /locus_tag="VFR82_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEU4502140.1"
                     /translation="MRTGLVYHPAYLEHDMGAGHPESPNRLRAIMQQLEQSGTAAHVT
                     RIEPRKAEDEWITQIHSPGYVAALNRHSPTSGRVSLDPDTSMSPGSLSAAYLAAGGAL
                     AGVDAIMQGQVDHVFCAVRPPGHHAEADRAMGFCLFNNVAIAARYLQKKYGLSRVLVV
                     DWDVHHGNGTQHSFEQDPSILFFSTHQYPHYPGTGRASEHGTGAGEGFTINVPMEAGE
                     GDDEYRATFLQALLPAAEEFKPDFVMISAGFDAHRDDPLAGMGLTEAGYADLTALVAG
                     VAKRHAQGRILSCLEGGYNLTALAASVDAHLKALLAA"
     gene            5736..6056
                     /locus_tag="VFR82_10855"
     CDS             5736..6056
                     /locus_tag="VFR82_10855"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4502141.1"
                     /translation="MPNPRIEPLKKVLGMDPNDEVAWFGLGKAYMDDGNFQEAANALR
                     QCITVKPTYSAAYYALAQSLHKLSLLDECRTVCSTGIEVSTKNGDAMVTKNLEQLRTS
                     LQSS"
     gene            complement(6044..6886)
                     /locus_tag="VFR82_10860"
     CDS             complement(6044..6886)
                     /locus_tag="VFR82_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502142.1"
                     /translation="MARFRPRKTFRLVSHLRDGHREPLPGWKREEQGLARDLEERWNT
                     LHRHCADQSSHLRLARLVRRLTHGDYGVRESALAELELATQLIRIGAKVTFLPESQAR
                     TADLECRVGRERFFVEVTAMVGSSGRQRIPRRQLPDDEGSEGMEPGAILIHRILARIQ
                     QKTKQLTDYADAVVLSISIPRADLRGERTVRSEALWLDLKALAGSVTLLLTKLRMLSA
                     VMISLWDVEPMPAQAGTRLANVELVERSKQQRGFPRVRILIRNPAAAVPLSERQQQGF
                     CQLL"
     gene            complement(6889..7218)
                     /locus_tag="VFR82_10865"
     CDS             complement(6889..7218)
                     /locus_tag="VFR82_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502143.1"
                     /translation="MNDHEINRAVQYVTASTCYGRETVATILKTGCGELTDLAVQSTR
                     QFERDALLEYVSQWTIRRTGQPEPLVREVLSCAGRWLDEVYDELSSTPPEPPGLPPQD
                     DEGAEPV"
BASE COUNT         1568 a         2169 c         2037 g         1523 t
ORIGIN      
        1 gcgaggccgc agccaaaaaa caaagtgggc acgatcagcg caagaatgaa ggtgcaggag
       61 cccgtgaacc gaaaccacgc ttcttccggt ggccgcgaca tcgcgtggag agtagcactc
      121 cgttcgcacg gccgcaatgg atgcatcccg cgactgtagc cggatcactt tggaggccaa
      181 cgcttgcgtt gaaccgctgg tgtggaatgg atggagacgg tgctggcgcc cagtacgtgg
      241 acgccgcctc ctttgcgggc acggtgtgta caggacccgt tcagttgagg gaggggtcga
      301 agggcatatc ggcgtacact cgaaactcct cgcccaacgt ggcgaggatt tcggaccaca
      361 cgcgagtggg atctttctca aagaccgtgg cgggatcagc cggaacggtg atccaagacc
      421 cgattttcat ctcagactcg agctgccccg gaccccagcc ggaatagccg acgtaggcgc
      481 ggaatgcttc tttgccgtga tcggttgtga gaatcttatc caccaattcc atatccccac
      541 ccaaacagac gccgtcaaag acatggtggg agttgtcagg caattgatcc aaccggtaca
      601 gcagcatgac ctgattgggc tgcaccggcc caccggaaaa caggacatga cgctgtcctt
      661 ccaacaccgg catctgtggt aaggcttcgg aaacagacat cgccgtcggc cggttcacga
      721 ccacgcccag ggcgccttcc gctccatgct cacagagcag gaccactgtc tgtctgaagt
      781 tgggatcgcg gagattcggc gcggccacca gaaagatgcc tttgccgagt tgcgccttca
      841 acggaatctg catgatgaga aatatcctat cctgccgatt cgccacacgc aacccagcaa
      901 aagacggggc ccataaactt accggtaaaa cgtttcgcgg cggtcccgga gcagatcgtt
      961 gtacggggtg agcgatttat tgcgggcttc agccggatcg atatcgacca cacacagcgc
     1021 ttcctgatca cgcggggcac gctggagaat ggcgccccgc ggagaaacga cttcgctgtt
     1081 gccgatgtag gtcagccggt ccttgccttc gcgcgcctcg ctgccggttc gattacatgt
     1141 cacggcaaaa acgcgattct cgagacagcg caccggcatg gaatccgggc agttcggcag
     1201 cacgagattg gaaggatgac agatgatatc cgcgccttta agcgccaaga cccttgcagc
     1261 ctcgggatag taccagtcga aacagatcat gacgccgatc ttggcgggtc cgacatccca
     1321 gacatgaaag cccgtgtctc ccggcgtgaa atacagcgtc tcctcgaaaa aaagatgcgt
     1381 cttacggtag ctcccgatga agccttgcgg agcaacaacg acggccgagt tgtaataggc
     1441 tgtacctgcc tgctcgggca acccggcgac aatcaccatc tgtctgcgct tcgcgagatc
     1501 cagcaggcgc ttcgtcgtct gtccggcggg aatcggctca gccagggcgc gcacttcgtc
     1561 catcgagaca aattgatacc cggaggcgca cagctcaggc aggacgagca ggtcggcttc
     1621 ggcgtcttcg agtttggccg caatgcggtc taagttctcg gcaaccttcc cgaactccgg
     1681 attgttttgg tagtacccaa cccgcattgc ttgctccgat tcacacgagg gttccaagac
     1741 gtggaagagg acttttctta tagatgcgag gatgagccga gaaacgttcc ctctcctgtc
     1801 cagcttacag acgacaacgg gcgggaaatc atcattcccg cccgtcatca gtacagcacg
     1861 gttggctgaa aacgttattt cacttcggac agatgagtca ccgcgccttc cgcatgttcg
     1921 gtcgcctggt cggcgtgacc ggccttgcca tgggtgatgg cttcctttaa gtgcttgatg
     1981 ccctcatcta aatgaggatt cttgacgccc gcggcctgcg cgtgcttcaa cgcttcttcc
     2041 gcatgctggg ccacagcatc ggcatggccc tgcttgccgt gattgacagc gcccttcgca
     2101 tgttcaacgg cttcgcttac gtgtttgttg tcggcccagg ccaccagcgg taggccaagc
     2161 agtgcgactg cggccatagc cgccgctcca cgtcggatgc tcatcgtcat gggttcctcc
     2221 tcatggtttg agtgccaagt cttgatttgt tgcaagaaat cgtacgtagc gaagtcacct
     2281 tacacagcca ctttttcact gtcaagaagg ccgggccggc gcctctattc ccaacacttt
     2341 ttgcaggcct tgaaagtcct tctcaacggg gcaggcaaaa tcgcggctcc actcccacca
     2401 ggagccgggg taattcctga ccttctgaaa tccggccagc ttcaggacaa aatacagcca
     2461 tgaagaccgt acgccgccgg tgcaataaca gacaatgtcg tgttggggct ccaagccttt
     2521 ttcttcaaga gcggctttga tggctgcgag gtctttcaca gtcgcattcg tattcaagaa
     2581 cccattccag gagagatgaa tcgcggaagg aatatggccc ggacgaggga tgccggacac
     2641 ttctttcccc aggtattctt cgagactcct cgcgtccagg atcttcatgt cggacggagg
     2701 atgcttgacc atcgcccgca actcgtcttt gccgatgatc gtgtgggtgg cctgcctgac
     2761 cgtaaagttt ccaggcttcg ggcttacccg accatgttcg aacggccgct gctctgttac
     2821 ccacttgacc caaccgccat ctagaatgcg catgttctta tgaccgaaat actcaagcat
     2881 ccagaacatt ctgccttcat cgccccagtt gtcgaacggg ttcgagtaga tcacgacttc
     2941 gctgtcgtcg ttcaagccaa gcgcgcgcat tttttgctcc atccggttca tatcgggatc
     3001 gagcaaccct ttggcgaccg cattagggtc actataatgg tgccaggtcg tgtgcactgc
     3061 cccgggaatg tggccgccat attcataagc ggcgttgccg cgcacgtcga tgacaacgag
     3121 cttcggtttt cccaactgct tgtgcaatgt ctcggtatca atgagtaacg gatgattcat
     3181 gagactcctt caaccccgcc cgacagtatc aaccgcgcag cttgacccat gctgcgaact
     3241 gccggcgacg gacggctgga cgttcgcatc cagggccgcc tttaatttat ctgtcaatgg
     3301 aaactcccgt tcgtagatga cgaagcggta gggatcgttc cccgtcagac cataccttcg
     3361 ttgcagcatc tcatgctgct tatccgtcac aatgtggtac tgcacgcgcg tcttcacttt
     3421 caagtcctcc gcgctgtcgg gcagctgata cgcaaactga tactcccggc tcgccaacgg
     3481 tagaaggcgg ttatcataca actccaggat ggccggctgc cacatgatcc agcgtcccat
     3541 tgtcgatgtt tcttccttga gcacatgacc aagcgtatct tcgaccgtga actcgacggt
     3601 gaaaaaccgg tcgggatcac ccgtcggaat tttgtgtccc gcgccggcat tgaccagcgt
     3661 caaggtaaag gccacacgat cgcccggctt gggcgacgga gggtccgcct tcacttcgac
     3721 agccaccgcc cgtttgatca tgtccggatc gtggccgccg cgccaaaggt gctgcctccc
     3781 acgtcggatg ggactgttcg ttgcgacagg cctgtcgatc tcaggcatgt gacagctctg
     3841 gcagataaat ccgcgctctt gcatgaagaa tttcccttca tactccgcgt aggtcccgca
     3901 aggacccacc cggtagaact gcgcagggcc ggagacgaca ttatggcagc gataacagag
     3961 ttgggcggtc cggaaattcg gatcaaattg ggtcgggtgc ggagcggcgg aatcctcgaa
     4021 tgggccgtaa atgatgccgt cccgcacgtg gcacgcagcg caggtgaccg attccttctg
     4081 atactccgcg tcgtaatgcg gattcggctc ctgaaccgct ttctcgactc gaccgcgagg
     4141 aatgtctgta atgacagtcg gctgctggtt ttccaacggt gaatgacaat tcaaacagat
     4201 ccaaacattc ttatccttct tccaataggc ttggaagtag ggatcctcgt acgcacgagc
     4261 gtgaatgctg gatttccatt cgtcataaat ttcgcggtgg cattggccgc aggactccgc
     4321 cttgaggctt gtcagtccgt ccggaacctt ctggaagggg atggcatggg cataatccga
     4381 gcgcagccca aaaatcacga ccggcttgat ttcggtgtaa tacaggtacg tcgcgccgaa
     4441 cacgaccagt agcccgatca ccatgaactt ccaacgcctc atgcagcaag caacgctttc
     4501 agatgagcat ccacggacgc ggcgagagcc gtgagattgt acccaccttc aaggcaggaa
     4561 aggatgcggc cttgagcatg gcgcttcgcc acgccggcca ccagagcggt caaatctgcg
     4621 taaccggctt cggtcaatcc catcccggcc agaggatcgt cccgatgagc atcgaagccg
     4681 gccgagatca tgacgaagtc aggcttgaac tcctcagcgg cgggaagtag agcctgcaag
     4741 aacgtggcac gatattcgtc gtcgccttct cccgcctcca tcggcacatt gatcgtaaac
     4801 ccttcgcccg cgccggtccc gtgctcggac gcccgaccgg tgcccggata atgcggatac
     4861 tgatgggtgc tgaaaaagag aatggaagga tcctgctcaa aactatgctg cgtgccgttt
     4921 ccatgatgaa catcccagtc gacgacgagg acccgactga gcccatattt tttctgtagg
     4981 tagcgtgcag cgatggccac gttgttgaac aagcagaatc ccatcgcccg gtcggcttcg
     5041 gcatgatggc cgggaggccg aacggcgcaa aacacatggt ccacctgccc ctgcatgatc
     5101 gcatcgaccc ccgcgagcgc gccgccggcg gccagatagg cggcagacaa ggaaccgggc
     5161 gacatcgacg tatccggatc gagtgaaacc cgcccactgg tgggggaatg tcgattcaac
     5221 gcggcaacat aaccggggct atggatctgt gtaatccatt cgtcctcggc ttttcgcggt
     5281 tcgatcctcg tcacgtgcgc cgccgtgcca ctctgctcca actgttgcat gattgcacgc
     5341 agacgattcg gggactcggg atggccggcg cccatgtcgt gctcaagata ggcgggatgg
     5401 tacacaaggc cggttcgcat aatacatcat cctcgcaaga cgctcaaaat ggcagccgtt
     5461 cttcccccac tcaattccgg cgtgaaacac gccttatccc gggcaaggcc gcagcgagtg
     5521 agctgccgag gagggacatg ccaatctttg cttgaaccgg tcgcttcgat cacatgcgag
     5581 cggatagtgc ttggcctccc tgcctcagct cgttgtggca ttcagcgatg cgagcacgaa
     5641 gctggcagct ttccagcgtc ttggaatgta gcatgcagga atcggcatag acaacgcgcg
     5701 tatggcactg ctatcgtcta cgctgaaagg aacacatgcc taatccgaga attgaaccgc
     5761 tcaaaaaagt cttggggatg gatccgaatg acgaagtggc ctggttcgga ttggggaaag
     5821 cctacatgga cgacggaaat ttccaagaag ctgccaatgc gctacggcaa tgcatcaccg
     5881 tgaagccgac ctactcagca gcctactatg cgcttgccca atcgctgcat aagctgagcc
     5941 tccttgacga atgccgaacc gtctgcagca cagggatcga ggtgtcgaca aagaacggcg
     6001 atgccatggt gaccaagaat ttggagcagt tgaggacctc acttcaaagc agctgacaaa
     6061 aaccttgctg ctgtcgttcg ctcaggggta cggcggcagc cggattccgg atgaggattc
     6121 gcacacgggg aaacccccgt tgttgtttcg accgttcgac cagctccacg ttggccaatc
     6181 tcgtaccagc ctgtgcaggc atcggctcga catcccagag tgagatcatc acggcgctga
     6241 gcatgcgcag tttcgtgagc aggagggtca ctgaccccgc cagggccttt agatccagcc
     6301 acaacgcttc tgatcgcacg gttcgctcgc cgcgcaaatc ggcgcgcggg atggaaatac
     6361 tcaagaccac cgcatcggcg tagtccgtga gctgtttggt cttttgctgg atgcgtgcaa
     6421 gaatgcgatg aatcagaatc gcacccggct ccatcccctc tgatccctcg tcatcgggaa
     6481 gctgacgccg tggaatccgc tgtcgtccgg acgatccaac catggcggtc acttcaacaa
     6541 aaaaacgctc gcggccaaca cggcattcca aatccgccgt tcgcgcctgc gattccggga
     6601 ggaaagtcac ctttgcgccg atacgaatca gctgggtagc caactccagt tcggcaagtg
     6661 ccgactcccg aacaccgtaa tctccatggg tgagccggcg gacgagacgg gccaagcgca
     6721 gatgactcga ttggtcggcg caatgccgat gcagggtgtt ccaacgctct tccagatcgc
     6781 gcgccaggcc ctgttcttcg cgcttccagc caggaagcgg ctcacgatgc ccatcacgca
     6841 gatgcgagac aagacggaag gtcttccgtg ggcgaaaccg tgccatatct agaccggctc
     6901 ggcgccctcg tcgtcttgtg gcgggagccc cggtggttca ggcggtgttg acgacaattc
     6961 gtcatagact tcatcgagcc accggccggc acaactcaac acttctcgga ccaacggttc
     7021 gggctgtcct gtccgtctga tcgtccactg tgagacatat tccagcaagg cgtcacgctc
     7081 gaactgccgt gtggattgaa cagccaaatc cgtcaattct ccacaacccg ttttgaggat
     7141 tgtggcgacg gtctctcgcc cgtagcaggt gctcgccgtc acatattgaa cggcgcggtt
     7201 aatttcgtgg tcgttcatag tatgacctct gaaggatttt cacatcctgc tcaacacgtg
     7261 tcaacgcgca aggcgggtcg actgggtttt tttctgc
//
LOCUS       DASTQY010000331         6036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_202127, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000331 DASTQY010000000
VERSION     DASTQY010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202127"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFR82_10870"
     CDS             <1..286
                     /locus_tag="VFR82_10870"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU4502144.1"
                     /translation="LVETAANILAFVPVGYLLVRSLSPNCGHPVFFACLLGFWSSVGI
                     ETYQLFCHDRVPSATDIFMNVAGTAVGVWLALAVDKAFTFCTFRIRRLST"
     gene            complement(684..1457)
                     /locus_tag="VFR82_10875"
     CDS             complement(684..1457)
                     /locus_tag="VFR82_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4502145.1"
                     /translation="MRQERIHIFDSQGEAYKQAFQIFLDHTDQKRNAKRWLQEVVDRL
                     PAHTVFIDAGAGNGELTKAFADVFKRTIAIEPNVYLLKQLQQSLPTVEVLGLPILAAQ
                     PAAQGDLVLCSHTFYYIPADEWLMHLERLVSWMAPTGVTIVVLQHCESGCMHMVRHFF
                     GHRFELRQVAEVFRAKHGDRYDVVTTLEPAHVETPSLDKAYAVAEFMLNLLDIKSAPA
                     REDVEGYVRTNFATEDGRYRIPVDQDFLQIRHRSPSCTT"
     gene            1633..2211
                     /gene="pyrE"
                     /locus_tag="VFR82_10880"
     CDS             1633..2211
                     /gene="pyrE"
                     /locus_tag="VFR82_10880"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU4502146.1"
                     /translation="MSALDELARSFHETHSFKWDREKGFTLASGYVSPFYVDCRALMA
                     HPSARRLVGQLAYEALTGVEIDCLGGLEIGAIAIATTISDYAFASSAPRQWRTFVVRK
                     QAKDHGLGKMIEGAVRSGDRALIVDDVLTSGGSLLKAITAAREAGLTVTGALVIVDRK
                     EQDGRAKVEQCGVKVTSLLTIDDLIAQQSRRS"
     gene            complement(2225..2536)
                     /locus_tag="VFR82_10885"
     CDS             complement(2225..2536)
                     /locus_tag="VFR82_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502147.1"
                     /translation="MKTWSSLVCVLVLCAAGCAEGAKLAQANEDGGVVTYPFKGDQGH
                     MLSSFRKDALALMKDTCGGGYLIVREGEARGRARVVGGGVEGGQEIVRERRWGIEFRC
                     K"
     gene            2589..3002
                     /locus_tag="VFR82_10890"
     CDS             2589..3002
                     /locus_tag="VFR82_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU4502148.1"
                     /translation="MIRRIAMTLLGLLLVTGLTTTVTAHHSYLLSVQQLRASLNKAPS
                     MAQKGFVLIDVRSPEEHAAGFIPGTDLNIDFREIKTRHREIGVSLDEHVVVYCQSGHR
                     SNIAAETLADLGYTHAYNVRGSMNAWVEAGYPVTR"
     gene            complement(3023..3889)
                     /locus_tag="VFR82_10895"
     CDS             complement(3023..3889)
                     /locus_tag="VFR82_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502149.1"
                     /translation="MKPLDDTLRVCALLIIVLCLSGFALAPAWAQTITWDRPSEGLAI
                     GVWNPSTSCPDVPPLLVSEIDPLRYRVAVHYFRNEPLSEPPDILEWQRRTGHDLVFNA
                     GLFRENFSYLGLLYGKGKPMGGKRHASWMGLFVAEPTAGGPSTAGILDLSIDSFDEQQ
                     PAYGEAAQSLMLLDRAGKVRVNQTGKIAQQTIVGELRNGHFLIMKTTEMTSLHAIGQC
                     LRDAYPTIRQAMAMDGGSSSDLMISPALRQAVQKTTGTHEWMTLVNSGPTGHVGLPAV
                     IGISPRHQSGRP"
     gene            complement(3944..5560)
                     /locus_tag="VFR82_10900"
     CDS             complement(3944..5560)
                     /locus_tag="VFR82_10900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502150.1"
                     /translation="MKTPLRLLLLEDDPVDAELVAATLSEAGVVCTAQRVDTREAFLN
                     ALRVGGYDLILADYSIPGFDGMTALGLARDKAPEIPFLFVSATIGEELAIDAMHQGAT
                     DYVLKQRLGRLVPSVLRALRERDERRERKKAEDALVQSEKQFRQAQKMEAVGRLAGGI
                     AHDFNNLLTVIMGYSHVLSTELGSEHPLHSKIEETQKAGEKAAMLVRQLLAFSRKQPL
                     EPKHLSVNNVVSNLEGMLRRLIGADIRLVIRLDPSNSQVLADQAQLEQVLMNLVVNAR
                     DAMPHGGTLTIETTQTELAKSPLYHVDPLPPGHYVKLSVTDTGSGMDRNTQAHIFEPF
                     FTTKEEGKGSGLGLSTVFGIVTQSGGGIDVNSRVGYGTKFDIFFPRVAADAQAASARR
                     AGEQPIGGTETILLVEDDASVRILLRDSLRKLGYRVIEAKHGIEACLLASQELANLHL
                     LLTDMVMPGMGGRELAQHLMTMKPDLRVLFMSGFTDDVGILAGHEQGTSGFLQKPFTP
                     ELLARTVRKILDAPSTNASQPTVNRASSSG"
     gene            complement(5622..>6036)
                     /locus_tag="VFR82_10905"
     CDS             complement(5622..>6036)
                     /locus_tag="VFR82_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502151.1"
                     /translation="EDNPRDAELALAAMEEHHIADKVVVCHDGAEVLDYLYCRGPFKS
                     RQWGNPVVVFLDLKMPKVDGIEVLRTIKNDANLKPIPVVMLTSSREERDLAQSYALGA
                     NAYVVKPVEFQQFISAVKELGVFWGVINEPPPEGS"
BASE COUNT         1263 a         1705 c         1713 g         1355 t
ORIGIN      
        1 cttggtcgag acggcggcaa acattctggc gtttgtcccc gtaggctatc tcctcgtacg
       61 aagcctttcg cccaactgcg ggcatccagt attcttcgcc tgcctcctgg gcttctggag
      121 ctcggttggc atcgagacct atcaactctt ttgtcatgat cgagtccctt cagcgaccga
      181 catattcatg aacgtcgcag gaacggccgt aggtgtctgg cttgctctcg ctgttgataa
      241 ggctttcacg ttttgtacct ttcggatccg ccgtctttcc acctaacccg ctgcctcaga
      301 taaatcgaga ggctgataca gcttcgcctt ctgacttaca tgagggcgcc tcttacctaa
      361 cggcgcttag gtcgtctgcc gtcaagctat ggcgcgtcgc tctacacgtc ggatgaccat
      421 gctccaacga ttcagaagac tgaaagacgg agcgatggct ctccgaagga caggactgat
      481 gccgtgatga tatggatcag gctactgttt catccggcga cgtagagtgg atcctagcag
      541 tggtgcggga aagcgcaatc aatgagaagt aaaaatgatg taaacatttg atgcgcgggg
      601 atacggtcag ctatgccgag gtgaaggcca ggcaggatga gctgaccgtc aaggtgtatc
      661 gcagaattgg agaaggtcca acttcacgtg gtgcaacttg gagatcggtg gcgaatctgg
      721 agaaaatctt gatccaccgg aatgcgatac cttccgtcct ccgtggcgaa gttcgtccgg
      781 acgtaccctt ccacgtcttc cctcgcaggc gcggatttaa tgtcgagcaa gttcagcatg
      841 aattcagcga cggcgtatgc cttatccaga cttggagtct cgacgtgcgc aggttccagc
      901 gttgtcacaa cgtcatagcg gtcgccgtgc ttagcccgaa acacctctgc gacctggcgc
      961 aactcaaacc ggtggccaaa aaagtgacgc accatgtgca tgcacccgct ctcgcaatgc
     1021 tgcaacacca ctatagtaac gccggttggc gccatccagg agacgagacg ttcaaggtgc
     1081 atcaaccact cgtccgcagg aatgtaatag aacgtatggg agcagagcac cagatcgccc
     1141 tgtgcagccg gttgagccgc caggatcggc aagccaagca cctcaaccgt cggtaggctt
     1201 tgttgcagct gcttgagaag gtagacgttt ggctcgatgg caatggtgcg cttaaagaca
     1261 tcggcaaacg ccttggtgag ttctccattc cccgcgcctg catcgatgaa gactgtgtgc
     1321 gccggtaacc tatcgaccac ctcctgcagc caacgcttgg cgttccgttt ttgatccgta
     1381 tgatcaagaa aaatttgaaa ggcctgctta taggcttctc cttgagagtc aaatatgtgg
     1441 atgcgttctt gcctcatgcg gcacgcctct taacagcaaa aattggcaat ggcgcggcta
     1501 ctatacgccc ttttgttccc ccaatcactt gccctcagac ggaacatgtc tccgtaggct
     1561 ggaccgtgct tatgcatgtg gtgtacagtc caacgccatt ctaaggggtt cgaaccagca
     1621 ggagtgaaca tcatgtcggc cttggatgaa cttgcccggt cctttcacga gacccactca
     1681 ttcaaatggg acagggaaaa gggattcacg ctcgcatccg gctacgtcag cccgttttac
     1741 gtggattgcc gtgccttgat ggcgcatccg tccgcacgtc ggctggttgg ccagcttgca
     1801 tatgaggcac tgactggagt ggagatcgat tgtctgggag ggcttgagat cggcgccatc
     1861 gccattgcga cgacgatctc ggattacgcc tttgcatcaa gtgcacccag acaatggcga
     1921 acgttcgtgg tccgcaagca ggcgaaggat catggcctgg gaaagatgat cgaaggtgcc
     1981 gtcaggtctg gcgatcgcgc cctcattgtc gatgatgtgt tgacgagcgg tggttcgctg
     2041 ctgaaagcga tcaccgcggc aagggaggcg gggctgacgg tcacgggggc gctggtgatc
     2101 gtggatcgga aagagcagga tgggcgggcc aaggtggagc aatgcggggt gaaggtcacc
     2161 agtttgttga cgatcgacga cttgatcgct caacaaagcc gcaggagctg agtacgaatt
     2221 tgtcctactt gcagcgaaac tcgatgcccc agcgacgctc tctgacgatc tcctgtcctc
     2281 cctcaacacc tcctccgacc acgcgcgctc gtcctctcgc ttctccttca cgcacaatca
     2341 gatagcctcc gccacacgta tccttcatca gcgcaagagc gtcttttcga aatgacgaca
     2401 gcatatggcc ctgatcgcct ttgaagggat aggtcaccac gccaccgtct tcgttggctt
     2461 gggctaactt tgccccctct gcgcatccgg ctgcgcagag cacaaggaca catacgagac
     2521 ttgaccaggt cttcattcgg ctcctatgat aatcattact tcagacacgt tgtatgtagg
     2581 agggcaccat gattcgtcga attgccatga cgttactcgg tctgttgttg gtcaccggcc
     2641 tgacgacgac ggtcaccgcc catcattcgt atttgctgtc ggttcaacaa ctgcgcgcga
     2701 gcctgaacaa agcaccgtcg atggcacaga agggcttcgt gctgatcgac gtccgttcac
     2761 cggaggagca tgccgcggga tttattcccg gcactgatct caacattgat tttcgcgaga
     2821 tcaagacgcg ccatcgcgaa atcggcgtct ctctggacga gcacgtcgtg gtctactgtc
     2881 agtccggtca ccggagcaat atcgcggcag aaacgctggc ggaccttggc tatacccacg
     2941 cgtataacgt ccgggggagt atgaatgcct gggtcgaggc cgggtatccg gtcactcgtt
     3001 aatacacctg caaacagtcc actcagggtc gtccgctctg atgacggggg ctgatgccga
     3061 tcacggccgg caggccgaca tggcccgtgg gtccggagtt caccagcgtc atccactcgt
     3121 gagttcctgt cgtcttctgt acggcttggc gaagagcggg actgatcatc aggtctgaag
     3181 aagaccctcc atccattgcc atcgcttgac gaatggtcgg gtacgcgtca cgcaggcatt
     3241 gcccgattgc atgaagcgag gtcatctctg tcgtcttcat gatgagaaaa tgcccgttac
     3301 gcagttctcc aacaatggtt tgttgggcga ttttgcctgt ttgattcaca cgtaccttcc
     3361 ccgctcggtc cagaagcatg agcgactggg ccgcctcccc gtacgccggc tgctgctcat
     3421 cgaacgaatc gatcgacaag tccaagatgc cggctgtcga tggaccaccc gccgtcggtt
     3481 ccgcgacaaa cagtcccatc catgacgcat ggcgcttgcc tcccatcggt ttgccctttc
     3541 catacaagag tccgagataa gaaaagttct cccggaagag gccggcattg aagaccaagt
     3601 catgaccggt ccttctttgc cactcgagaa tatcaggcgg ttcggagaga ggttcgtttc
     3661 gaaaatagtg gacagcgact cgatagcgaa ggggatcgat ctccgaaacc aggagcggcg
     3721 gcacgtcagg gcatgacgtc gacggattcc agaccccgat ggccaatcct tccgatgggc
     3781 ggtcccatgt aatggtctga gcccaggccg gcgccagtgc gaaacctgac agacagagga
     3841 cgataatcag cagtgcgcaa acgcggagag tgtcatccaa tggtttcatg cgcttcaacg
     3901 gagtataggg atggtccgat tcagcgtcaa actcggatgg cagttaccca gagctggagg
     3961 ctcggttgac agttggttgg gaggcgttgg tcgaaggcgc atcaagaatt tttctcactg
     4021 tgcgcgccaa gagttccggc gtaaacggct tttgcaggaa cccgctggtg ccttgctcgt
     4081 gaccggccag gatgccgacg tcgtcggtaa agcctgacat gaagagcacg cggagatccg
     4141 gtttcatggt catcaagtgt tgggcaagtt cccttccccc catcccgggc atcaccatat
     4201 cggtgagcag cagatgcagg tttgccaatt cctgcgacgc cagcaggcag gcttcgatcc
     4261 cgtgcttggc ctcgatgacc cgataaccga gctttctcag gctatcacgc aggagaatgc
     4321 gcacactcgc gtcgtcttcg accaacaaga tagtttctgt accgccgatg ggctgttcgc
     4381 ccgcgcgccg ggcggacgcc gcctgcgcat ccgcggcgac tctggggaaa aaaatgtcaa
     4441 acttggtgcc ataccctacc cgactgttga catcgatgcc gccaccggat tgagttacga
     4501 tcccgaacac cgtggaaaga ccaagcccgc ttcccttccc ttcctccttc gttgtgaaga
     4561 agggttcgaa gatatgggct tgagtgttcc ggtccatgcc actgccggtg tctgtgaccg
     4621 acaatttgac gtagtggcca ggcggcagag gatcaacatg gtagagcgga cttttggcta
     4681 actccgtctg cgtcgtctca atggtcagtg tgccgccatg cggcatcgca tcccgtgcgt
     4741 tcacgacgag gttcatgagt acttgttcca gttgggcttg atcggccagg acctgactgt
     4801 tggaaggatc gagcctgatg acgagacgaa tatcggcccc gatcaagcgt cgcagcatcc
     4861 cttcgagatt ggacactaca ttgttgacac tgagatgttt gggctctagc ggctgcttgc
     4921 ggctgaaggc cagcagttgc cggacgagca tggcagcctt ctcgcctgct ttctgggttt
     4981 cttcgatctt gctgtggaga gggtgctcgc ttcccaattc ggtcgagagg acgtggctgt
     5041 atcccatgat caccgttagg agattgttga aatcgtgtgc gatccctcct gcgagccgtc
     5101 ccaccgcctc cattttttgg gcttgcctga actgtttttc gctttgaacc agcgcgtctt
     5161 cggctttctt ccgttcccgt cgctcgtcgc gttcacgaag tgcgcgcagc acggaaggaa
     5221 cgagtcgtcc caaccgctgc ttcagcacgt agtccgtggc accttgatgc atggcatcga
     5281 tggctaattc ttcaccgatg gtcgctgaga caaagaggaa agggatctcc ggagccttat
     5341 cgcgggccag cccgagggcg gtcatcccgt caaagcccgg aattgagtag tcggcaagga
     5401 tgaggtcata cccgcccacc cgcaatgcgt tcaggaatgc ctcccttgtg tccacacgct
     5461 gggcggtaca gacaacgccc gcttcggaca gggtggcggc aacgagctcg gcatcgacgg
     5521 gatcgtcctc gagaagcagc agacggagcg gggttttcac aggtcctctc gggcaggctt
     5581 cgcgaatgtt gttaacgata gtggcgttgt gtgcagcggg tctagctccc ctcggggggt
     5641 ggttcgttga tcactcccca aaagacgccg agctccttga cggctgaaat aaattgctgg
     5701 aactcgaccg gtttaacgac ataggcgttg gcgccaagcg catagctttg ggcgagatct
     5761 cgttcttcac gtgacgatgt cagcatgacg accgggatcg gcttgagatt cgcgtcattc
     5821 ttgatggtcc tcaagacttc aatgccgtca acttttggca tcttgagatc aagaaacacc
     5881 acgacgggat tgccccattg ccggctctta aagggtcctc gacagtacag ataatccaac
     5941 acctctgcgc cgtcatgaca gacgaccact ttgtcggcaa tgtgatgctc ctccatggcg
     6001 gcgagcgcca attcggcatc ccgaggattg tcttcc
//
LOCUS       DASTQY010000332         6182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_207829, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000332 DASTQY010000000
VERSION     DASTQY010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207829"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VFR82_10910"
     CDS             <1..502
                     /locus_tag="VFR82_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEU4502152.1"
                     /translation="GLPEYDAAVLTASKDMADYFEAATQLFNQPKTVSNWVMGELTRE
                     LNNSGTTVAASPVSPHRLVTLLEMVEKGTISLKVARDIFPELYRSEKSPEQIVQEQGL
                     TQVSDEEALSTIIDEMLLNNPVQVAQFKEGKQQVLGYLVGQAMKASGGKANPSKVNEL
                     LKKKLG"
     gene            534..1823
                     /gene="eno"
                     /locus_tag="VFR82_10915"
     CDS             534..1823
                     /gene="eno"
                     /locus_tag="VFR82_10915"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU4502153.1"
                     /translation="MSTIREIKGRQVIDSRGNPTVEAEVTLESGAKGRAAVPSGASTG
                     EKEAIELRDGDKKRWMGKGVSKAVGNISKTIAPELLGRDALDQVGIDQAMIELDGTKT
                     KGKLGANAILGVSLAVAKAAAIETGQPLYRYLGGTNARVLPVPLMNIINGGAHADNRL
                     DLQEFMIVPIGASHFSEAVRMSTEVFHSLKALLKKKGLSTAVGDEGGFAPDLQSNEEA
                     LTLIMEAIEEAGYQPGQDMALALDCAASELYDKNRYVLEAEKTRERSSEEMIAYYGKL
                     LDRYPILSIEDGLSEVDWKGWRLLTEKLGERVQLVGDDIFVTNVEIFAKGIDEGIGNS
                     ILIKLNQIGTLTETLEAIELAKRSGYTAIISHRSGETEDTTIADVAVATNSGLIKTGS
                     LSRTDRVAKYNQLLRIEEELGAAAVYRGREAMPGRGR"
     gene            1830..2180
                     /locus_tag="VFR82_10920"
     CDS             1830..2180
                     /locus_tag="VFR82_10920"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU4502154.1"
                     /translation="MIVKRNRGREWLDWQRRMVTGAHYVAAGMCLLLLLALCFGEMGL
                     PRFFSMRDHAQELERDIQDLQRTTKMLRGDIERLERDPARIEQLARERLGYVRKGETV
                     YQLVPEDGQERSRP"
     gene            2177..3061
                     /gene="era"
                     /locus_tag="VFR82_10925"
     CDS             2177..3061
                     /gene="era"
                     /locus_tag="VFR82_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU4502155.1"
                     /translation="MKFGTVAIIGRSNVGKSTLLNRLLNEKIAIVSEKPQTTRTRILG
                     VVHAPGAQIALLDTPGLHKPQHLLNRRMVRTAVETLEEADVLFVIMDATSLPGPGDLL
                     VLDHVRGAVNKRGRPVLLVLNKIDLVNKIKLLPVIESYARLFAWTEVVPVSAQTGDNV
                     ERLLAVAVSQLPEGEAAYDDDTVTDQTMRTLAAEMIREKLLRQTHEEIPYSLAVEIDE
                     FIEEGKLARITASVLVERESHKAIVIGKHGERLKTVGTEARLDMERVFGMKVFLQLWV
                     KVREAWREDEHALIELGY"
     gene            3198..4658
                     /gene="mgtE"
                     /locus_tag="VFR82_10930"
     CDS             3198..4658
                     /gene="mgtE"
                     /locus_tag="VFR82_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEU4502156.1"
                     /translation="MQPTEKVPDVRPIEPKPRVGDKDVLRNAPKEQADRGHNKSDIVL
                     LSVQRLLRRGAITNLAKMLGRMHPADVAKIIVHLSSPKEKREVFELVRGDSKRGQVLS
                     ELDTDSITQVLSDLLQSDIAWLIKDLAPDDVAYILGVLPEERAKEILSLMRTEDSTEV
                     ADILKYPKDTAGGIMTTEFFALSEDATAQDAIRRLQQATDAEMVFYIYVTDKNDHLVG
                     VLSLRQLLTVPPATPLKNIATREVISVSVDMDQEEVARQVASYNLLAIPVVDKEGVLV
                     GIITVDDVVDVIREEATEDMLKMAGAVEEDAVSKSSSVAAAKVRLPWLFTNLVGSLLS
                     GAILWEFRYTIQEVVAIVSFIPVIAAMGGNVGLQSSTLIIRGLATGLVEVTDVWNVFF
                     REVKIGLLMGVACGVILSLVGWIWHQGFLGVVVGVSLITAFLVSTSMATFMPIVLKRM
                     GVDPAVAAGPFVTTANDITGITIYLTLATIFMEYLR"
     gene            4700..5452
                     /gene="recO"
                     /locus_tag="VFR82_10935"
     CDS             4700..5452
                     /gene="recO"
                     /locus_tag="VFR82_10935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU4502157.1"
                     /translation="MPLVKTPAITLKSRKWGEADRIVTFYAKHLGKIRAVARGARRLK
                     SRMGAALEPLTLCELNLFEKSGDSLYRVSQVDLVEPFVRFRENLTLMAAAARMTNVVA
                     AVTPDGDPDPHLFETLEQGLRTLVTSEDPALTALLFQIRLLGLTGFRPQTDHCAACGK
                     TSVIDEPQFSPVSGGLVCAACAARQRVPCLPLSRGSLAFLHQALQLAPIMLTRLKAAG
                     QVRGEVEDAIEGYVTVVAGRRIPPVDFLVQPL"
     gene            5483..5839
                     /locus_tag="VFR82_10940"
     CDS             5483..5839
                     /locus_tag="VFR82_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEU4502158.1"
                     /translation="MADTQLEPRDLSWLEKGVLGIAWSDGHNGVYPVRHLRLHCPCAA
                     CVDEWTGVRRLKADDVPLLIMLQDIESVGRYALQFKWSDGHDTGIYSYKLLRELCQCD
                     VCQPVKPNEPKSRRLL"
     gene            5836..>6182
                     /locus_tag="VFR82_10945"
     CDS             5836..>6182
                     /locus_tag="VFR82_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502159.1"
                     /translation="MTLRRRVLGVGLIAVYGLLVMSGCTGLSLAEQEQKIRNNDLILR
                     KLEPRAFVRAWGMPTYQRMEFMPFFGMKDGSLVPRSRLPVGASPPGWEAGVDAGEALF
                     LAYPDRGWLIVFL"
BASE COUNT         1369 a         1594 c         1933 g         1286 t
ORIGIN      
        1 cggtctgccc gagtatgacg ccgcagtgct cacggcctcc aaggacatgg cggactattt
       61 cgaggcagcc acccagctgt tcaatcagcc gaagacggtc agcaactggg tcatgggtga
      121 attgacgaga gagttgaaca atagcggcac gaccgtcgca gcctcgccgg tttctcctca
      181 tcgtctcgtc acgttattag agatggtgga gaaggggacg attagcttaa aggtcgcgcg
      241 tgacattttc cctgagctct atcgcagcga aaaatcgccc gagcagatcg tgcaagagca
      301 aggtctgacc caggtgtccg atgaagaggc gctttcaacg atcatcgacg agatgctgct
      361 gaacaatcct gtccaagtag cccaattcaa ggaaggcaag cagcaagtgc tgggatattt
      421 ggtcggtcag gcgatgaaag caagcggagg gaaggccaac ccaagcaagg tcaatgagtt
      481 attgaaaaag aagttgggat gaaggagcga gccagcaggg ggagaacgag agcatgagca
      541 cgatcagaga gatcaagggc aggcaggtca tcgattcgcg gggcaatccc accgttgaag
      601 cggaagtgac actggagagc ggggcgaaag gccgggcggc ggtgccgtct ggcgcatcaa
      661 cgggggaaaa ggaagcgatc gagctgcgcg acggagacaa aaagcgctgg atgggcaagg
      721 gcgtgtcgaa ggcggtgggg aacatcagca agaccatcgc accggaattg ttgggaagag
      781 acgcccttga tcaggtcgga atcgaccagg cgatgatcga gttggacggt accaagacga
      841 agggaaagct cggggccaat gcgatcctgg gcgtctcgct ggctgtggcc aaggccgctg
      901 ccattgaaac cggccagccg ttgtatcgct atctcggtgg aaccaatgcg cgggtgcttc
      961 cggttcccct catgaatatt atcaacggcg gagcccacgc cgacaatcgg ctggatcttc
     1021 aagagttcat gatcgtgccg atcggagcaa gtcatttcag cgaggcggtt cggatgtcga
     1081 ccgaagtgtt tcattctttg aaggccttat tgaaaaagaa aggcctcagt accgccgtcg
     1141 gcgatgaagg cgggtttgcg ccggaccttc aatccaatga ggaagccctc acgttgatca
     1201 tggaagcgat cgaggaagcg ggctatcaac cgggccagga tatggcgctg gcgttggact
     1261 gcgcagccag cgagctgtat gacaagaatc gctatgtgct ggaggcggag aaaacacggg
     1321 agcgctcgtc ggaagagatg attgcctatt atggcaagtt gctggatcgt tatccgatcc
     1381 tctcgatcga agacggcttg agcgaggtcg actggaaggg atggagactg ctcaccgaga
     1441 aactgggtga aagggtccaa ctcgttggcg acgatatctt tgtgaccaac gtcgagattt
     1501 tcgccaaagg catcgatgaa ggcatcggta attcgatcct gatcaagttg aatcagatcg
     1561 gcacattgac cgaaacgttg gaggcgatcg aactggccaa gcggtcgggc tatacggcga
     1621 ttatttcgca tcgatcagga gagaccgagg ataccacgat tgcagatgtt gcggtagcga
     1681 cgaacagcgg tttaatcaag acaggctctc tgtcccgcac tgatcgggtg gcaaaataca
     1741 accaactcct tcgaatcgag gaagaattgg gcgccgcagc cgtctatcgt ggacgtgagg
     1801 cgatgccggg tcgcggccga taggcacgta tgatcgtcaa gcgcaatcgc ggacgagagt
     1861 ggttggattg gcaacggcgg atggtgacgg gcgcccatta tgtcgccgcc ggcatgtgcc
     1921 tgctgttact cctcgccttg tgctttggtg agatgggtct gccgcgattt ttttccatgc
     1981 gcgaccatgc tcaagagctg gaacgggaca tccaggacct tcagaggacg acgaaaatgt
     2041 tgcgcggaga catcgagcgc ttagaacgtg atcccgcacg gatagaacag ttggcccggg
     2101 aacggctggg gtatgttcgc aaaggcgaga ccgtgtatca actcgttccg gaagacggac
     2161 aggagcgatc gcggccatga aattcggtac cgtggccatc atcgggcgat ctaatgtggg
     2221 caagtccacc ttgctaaatc gcttgctgaa cgagaagatt gccatagtct cagagaagcc
     2281 gcagacgacg agaaccagga ttctcggtgt cgtgcatgca ccgggagcgc agatagccct
     2341 gttagatacg cccgggcttc ataagccgca gcatctcttg aatcggcgta tggtccgcac
     2401 ggccgtggag acactcgagg aagccgatgt cttatttgtc atcatggatg cgacgagttt
     2461 gcccggaccg ggtgatctgc tggtgctgga tcatgtgagg ggcgcagtga acaagcgagg
     2521 gcgcccggtc ctcctcgtgc tcaataagat cgacctcgtg aacaagatca agctgctccc
     2581 cgtgatcgag agctacgcga gattgttcgc ttggacggaa gtcgtacctg tgtcggctca
     2641 aacgggcgat aatgtcgaac ggttgctggc cgtcgcggtt tcacaacttc ccgaaggaga
     2701 agcggcctac gatgacgaca ccgtcaccga ccaaacgatg cggaccttgg ctgccgaaat
     2761 gatccgggaa aaattattgc ggcaaactca tgaagagata ccctattcgc tggccgtaga
     2821 gatcgacgag ttcatcgaag aaggcaaact cgcacgcatt accgcttcag tgctggttga
     2881 acgagagtcg cataaagcca tcgtgatcgg gaagcatggc gaacgcctga aaacggttgg
     2941 cacggaagcg cggctcgata tggagcgggt gttcggcatg aaggtctttt tgcagctgtg
     3001 ggtcaaggtg cgggaagcgt ggcgtgaaga cgagcacgcg ctcatcgaac tgggctatta
     3061 acaggatgct gaaaaagccg cctttttcac ccgcccgctc cccggcgcgc cgagacgccc
     3121 ccttcgccaa gcttgtgctc gcgtcgcttc accctgtatg gcctgtcgag catagattgg
     3181 caacctaaag gcgaaacatg cagccaacag aaaaagttcc agacgtgcgt ccgatcgaac
     3241 cgaaaccccg tgtcggggac aaagacgttc tgcgcaatgc accgaaggag caggccgatc
     3301 gcgggcacaa taagtcggat atcgtgttgt tgtccgtcca acgcctgcta cgacgaggcg
     3361 ccatcacgaa tctggcgaag atgttgggcc ggatgcatcc ggccgacgtc gcaaagatca
     3421 tcgttcacct atcctctcca aaagagaagc gtgaagtctt cgagctagtc cgcggcgact
     3481 cgaagcgtgg ccaggtgttg tccgaactcg ataccgacag catcacgcag gtgttatccg
     3541 atttgcttca atcagacatt gcctggctga tcaaggatct cgctcccgac gacgtcgcct
     3601 acattctggg tgtcttgcct gaagaacgcg ccaaggaaat cctctcctta atgcgtaccg
     3661 aggattcgac cgaagttgcc gatatcctga agtatcccaa ggacacggcc ggcggcatta
     3721 tgaccaccga gttcttcgcc ttgtccgagg atgcgacggc gcaggatgcg attcggcgcc
     3781 ttcagcaggc gacagacgcc gagatggtct tttacattta tgtcaccgac aagaacgacc
     3841 atctggtcgg ggtcttgtct ctcagacagc tgttgacggt gcccccggcg acgccgctca
     3901 agaacattgc tactcgcgaa gtcattagtg tctcggtcga catggaccag gaggaagtcg
     3961 cccgccaggt tgcgagttat aacttattag ccatccccgt cgtcgataag gagggggttc
     4021 tggtcggcat cattaccgtt gacgacgtgg tggacgtcat ccgcgaggag gcgacggagg
     4081 acatgttgaa aatggccggt gccgtggaag aagacgcggt atccaagtcc tcgagcgtcg
     4141 ctgcggcaaa agtcaggctg ccctggttgt ttaccaatct ggtcggaagc ttactgtccg
     4201 gcgcgatcct ttgggaattc cgctatacga ttcaggaggt ggtggccatt gtgagcttca
     4261 tccctgtgat cgccgcaatg ggagggaacg tcgggttaca atcgtccacg ttgatcatcc
     4321 gcgggttggc gacagggctg gtggaggtga cggatgtctg gaatgtgttt ttccgcgagg
     4381 tcaagatcgg tctcttgatg ggtgtagcct gcggcgtcat cctctcgtta gttggatgga
     4441 tttggcatca gggctttctc ggggtcgtgg tcggagtgtc gctgatcacc gccttcctcg
     4501 tctccaccag catggctaca tttatgccga ttgtactcaa acggatgggc gttgatcctg
     4561 ccgttgcggc cggaccgttc gttacgacgg cgaacgatat cacaggcatc acaatctatc
     4621 tcacgctggc caccatattc atggaatatc tccgatagcg gtctcatcgc cttctgacac
     4681 attcgcacag ggacatggca tgccgctcgt aaagactccg gcaatcacct tgaaaagccg
     4741 caagtggggg gaggccgacc gcatcgtgac cttctatgcg aagcatctgg ggaagattcg
     4801 tgcagtggca cgcggtgcgc ggcgactcaa aagccggatg ggagctgccc ttgaaccgtt
     4861 gacgctatgc gaattgaatc tgttcgagaa gtcaggcgat tcgttgtatc gggtgtcgca
     4921 ggtggatttg gtcgaaccgt tcgtgaggtt ccgcgagaat ttgacgctga tggcggcggc
     4981 cgctcggatg acgaatgtag tggcagccgt cacgccggac ggcgatccgg acccgcatct
     5041 gttcgagaca ttggagcagg gacttcgcac attggtcacc agtgaggacc cagcgctcac
     5101 cgcgctgctg ttccagattc gactgctcgg tttgaccggc ttccgtccgc agaccgatca
     5161 ctgtgctgct tgtggaaaga cgagcgtgat tgacgagccg cagttttctc ctgtatccgg
     5221 ggggctggtg tgtgcggcct gtgccgcccg gcagcgggtt ccctgtctgc ccttatcccg
     5281 agggagtttg gcgtttctcc accaggcgtt gcagttggcg cctatcatgc tcacccgcct
     5341 caaggcagcc ggccaggtcc gtggtgaagt cgaggatgcc atcgaggggt acgtgacagt
     5401 ggtcgcagga agacggattc ctcctgtcga ttttcttgtg caaccgctgt gagatgtcgg
     5461 gctttcgagg ataccagtga acatggcaga cacccagctt gaaccgcgag acttgtcctg
     5521 gctcgagaaa ggagtattgg gaatcgcttg gagcgatgga cacaacggcg tgtatccagt
     5581 gcgccatctg cgcttgcatt gcccctgtgc ggcttgtgtc gatgaatgga caggagtgcg
     5641 gaggctcaaa gccgacgatg tgccgctgct catcatgttg caggatatcg aatcggtcgg
     5701 ccggtacgca ttgcagttca aatggagcga cggacacgat accggtattt actcctataa
     5761 gctgctgcgc gaactctgcc agtgcgacgt gtgccagccg gtgaagccga atgaaccaaa
     5821 aagccggaga ctcctatgac gctccgacgc cgtgttctcg gcgtgggatt gatcgccgtc
     5881 tatggcttgc tagtgatgtc aggatgtacc ggcctgtcgc tggcagaaca agaacaaaag
     5941 attcggaaca atgatctcat cctgagaaaa ttggagccgc gggcgtttgt tcgtgcctgg
     6001 ggaatgccaa cctatcagcg aatggagttc atgccatttt ttggtatgaa ggacgggtcg
     6061 ctcgtccctc gctcgagatt gccggttggg gcgtcaccgc ccggatggga agcgggggtt
     6121 gacgccgggg aggccttatt tttggcctat cctgaccgag gctggctgat cgtcttcctg
     6181 ga
//
LOCUS       DASTQY010000333         4095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1639663, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000333 DASTQY010000000
VERSION     DASTQY010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639663"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..656
                     /locus_tag="VFR82_10950"
     CDS             207..656
                     /locus_tag="VFR82_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU4502160.1"
                     /translation="MQSTTLSLILGVLSGLLFWGGSAIAEPSAEPSLKQSEGMQLSKG
                     VIGVSLDVSAERIGDPAILYVAMVNPEGPAHRAGLAHGDEIQAVDETPVAGKTYEQVV
                     KMIRGEPGTTVKLTVKGERGAREVSVTRVAADRLPVGPKGSHGSQAR"
     gene            662..1192
                     /locus_tag="VFR82_10955"
     CDS             662..1192
                     /locus_tag="VFR82_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502161.1"
                     /translation="MRLTAIILSLCVAINLVPAVPLSAEPARPSCGAVALMTKLDRYL
                     AAGDYRRALELAQREIDDAPSAASYIYLTYVYHAIDAYLAYLSQEESWTAVEHLYLNL
                     AYRDTEDLVDPPGGLARMAKEMIQTSVRQQSDVSAAMAARLDKTESDRLWLEQAGWRR
                     TNEKTWWQGVPSSWRG"
     gene            1202..1624
                     /locus_tag="VFR82_10960"
     CDS             1202..1624
                     /locus_tag="VFR82_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502162.1"
                     /translation="MKILSKGADIKLLGGLFIIVGMVDAIIIALFPDYALKLFGTAVT
                     GPVSLLTKLHSPLVHLLIGYGFVFLRPWGWGLAVAYSAFGLVSETMNQWSFGVTLIRS
                     GSMVAIALFASYLIWRRHRFVDEQPGEMSTTSVSQGSH"
     gene            1624..3069
                     /locus_tag="VFR82_10965"
     CDS             1624..3069
                     /locus_tag="VFR82_10965"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEU4502163.1"
                     /translation="MRGIVWFRRDLRLADQAALREACKDCREVIPLFIFDEPLLRSRE
                     FGSACVNFMLGCLKELASSLAASGMALQWRRGDPVEEMVRAAREWSADVVYWNRDYEP
                     DAIARDRNVSHRLGQAGIAVRTFKDHVVFETEEVRGVTGEPLQRYSAYRAHWWTRWHA
                     TKPALQGLPAVLSRKSGVPVPAFSPIPEAEELGYETVWPWIEPGERAAQARLRWFLEG
                     PIHRYVDGRNQPAMDGSSKLSPHFRFGTLSPLAAVHAALRVLAGGGHVSRPDVLTWVD
                     ELIWREFFQQVLASFPRVVDGPFRQASVPASRLPGLERDGLFQRWSEGRTGYPIVDAG
                     MRQLNQTGWMHNRVRMITASFLVKDLRIDWRSGERYFMNHLLDADVAANNGNWQWCAS
                     TGTDAMRGYRIFNPALQSKKFDPEGRYIREYVPELANVMNRYIHEPHLMSDEEQARVD
                     CRIGVDYPERIVDHRQAREDYLARGKAEAMK"
     gene            3066..4019
                     /locus_tag="VFR82_10970"
     CDS             3066..4019
                     /locus_tag="VFR82_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HEU4502164.1"
                     /translation="MTGQMLRLGISRCLLGDEVRFDGGHKHDHFLTDVLGRYVEWVPV
                     CPEVEAGLGTPREAMRLVGDPARPQLITVKSGIDHTEALERMSANRLQNLKRLSLSGY
                     IFKKDSPSCGIERVRLYNRSGMPSRQGVGIFARAFMNQFPLIPVEEEGRLCDPDLREN
                     FIERVFSYRRYQDLLVKSASRQSVVQFHTMHKYLLLSHSQPHYEALGRLVGQAHRYRP
                     KELAVKYGELFMKALAVKATVRKHVNVLQHIVGFFKTRLTTQEKRELLGVIDDYHRGL
                     TPLIAPLTLIRHYVTIFDVSYIHAQVYLNPHPKELMLRNQV"
BASE COUNT          864 a         1123 c         1272 g          836 t
ORIGIN      
        1 gggatcaata atgacaacgg ggcagagggt cgtcccggcc gttgcggaac gtcacgggta
       61 cagattccgc tttcgggaag ttcagcgagc gctacagacc atctttcccg caggggcata
      121 accatgaaaa aaagatcccc tatctcgacg gtctggcagc cggtattcta aaagaggaaa
      181 aacatttgaa tcgtaaggag ggagccatgc aatccaccac actatcattg attctgggcg
      241 tgctgtcagg actgttgttc tggggaggat ccgcgatcgc cgagccgtct gcggagccct
      301 ctttgaagca aagcgaaggc atgcagcttt ccaaaggagt gatcggcgtg tcgctcgatg
      361 tcagcgctga acgcatcggt gatccggcca tcctatatgt ggctatggtg aatccggaag
      421 gaccggctca ccgggcaggc ctggcgcacg gcgatgagat ccaagcggtt gatgagacgc
      481 cggtcgcggg caagacttac gagcaggtcg tcaaaatgat tcgtggagag ccgggcacga
      541 cggtcaagct cacagtcaaa ggtgagaggg gtgctcgcga agtgtcggtc acgcgggtcg
      601 ctgcagaccg tcttccagtg ggaccgaagg gctcgcacgg cagccaggcc cggtaggaat
      661 catgcgactc acggcgatca tcctgtccct ctgcgtggcg ataaatcttg tgccggctgt
      721 cccgttgtcg gcagaaccgg cgcgaccgtc atgcggcgcg gtcgccttga tgacgaagct
      781 cgatcgctat ctggcggcgg gcgattaccg tcgcgcactc gaactcgctc agcgcgaaat
      841 cgacgacgct ccttcggcgg ccagttatat ctatctgacc tacgtgtatc acgcgatcga
      901 cgcgtatctg gcgtacctct cgcaagaaga gagctggacc gccgttgaac acctgtactt
      961 gaacctggcg taccgcgaca ccgaggatct ggtggaccca ccgggcggcc tggcacggat
     1021 ggcaaaggag atgatccaga ccagtgtgcg tcaacagtcg gacgtcagcg ccgcgatggc
     1081 ggcacgcctc gacaaaacgg aatccgaccg cttgtggctg gagcaggcag gatggcggag
     1141 gacgaacgag aagacctggt ggcagggcgt gccgagttcc tggcgagggt aggaggtcac
     1201 catgaagatc ctctccaaag gcgccgacat caagttgctg gggggactgt tcatcatcgt
     1261 agggatggtg gatgcgatca tcattgctct gtttccggat tatgcgctga agctgtttgg
     1321 cacagcagta accggtcccg tgtctctgct gaccaagctg cattctcccc tggtgcatct
     1381 gctgatcgga tacggctttg tttttctgag gccctgggga tggggtctgg ccgtggccta
     1441 ctccgccttc ggactggtga gcgagaccat gaaccagtgg tccttcgggg tgactctcat
     1501 acggagcgga tccatggtag cgatcgcctt gtttgccagc tatctgatct ggcgacgcca
     1561 ccggtttgtc gacgagcagc caggcgagat gagcacaacg tctgtgtcgc aggggtcaca
     1621 ctgatgcgag gcatcgtttg gttcagacga gacctccgcc tcgccgatca agcagcactg
     1681 agagaagcct gcaaagactg tcgcgaagtc attccgctat tcatcttcga cgaaccgctg
     1741 ctgcgctcac gtgaattcgg ctcagcttgc gtcaatttta tgctggggtg cctcaaagaa
     1801 ctggcttcct cgctagccgc cagcggcatg gcgctgcagt ggcgccgagg cgatccggtc
     1861 gaagaaatgg ttcgtgccgc acgtgagtgg agcgccgatg tggtctattg gaaccgcgac
     1921 tacgagcctg atgccatcgc gcgtgaccga aacgtctcgc atcgactcgg ccaggcggga
     1981 attgcggtac gcacgttcaa ggaccacgtc gtgttcgaga ccgaggaagt gcgcggcgtg
     2041 actggtgaac ccttgcaaag gtacagtgcc taccgggccc attggtggac cagatggcat
     2101 gccactaagc cggctttaca gggtctccct gccgtactgt cacgaaagtc aggtgtaccg
     2161 gtccccgcct tcagtccgat tcccgaggcc gaagaactgg gttatgagac agtctggcca
     2221 tggattgaac cgggagagcg tgccgcgcag gcgcgattgc gctggtttct cgaggggccg
     2281 atacaccggt acgtggacgg ccgcaaccaa ccggcgatgg acggaagctc taagctgtcc
     2341 ccccatttcc ggtttggcac actgtcgcct ctcgcggcgg tacatgcggc cttaagggtc
     2401 ctggccggag gtggccacgt ctcacgcccc gatgtgctga cctgggtcga cgaactgatt
     2461 tggcgggagt tctttcagca ggtcttggcg tcgtttccgc gcgtcgtaga tggtccgttc
     2521 cgacaggcgt ctgtgcctgc ctctcgcctg ccagggttgg aacgtgacgg attgttccaa
     2581 cgctggagcg aaggcagaac cgggtatccg atcgtggatg caggaatgcg gcaactgaat
     2641 cagacaggct ggatgcacaa ccgggtgcgc atgatcacgg cctcttttct agttaaagat
     2701 cttcggattg actggcggag cggtgagcgg tacttcatga accatctgct ggatgccgat
     2761 gtggcggcca acaacggaaa ctggcagtgg tgtgcctcca ccggaacgga tgcgatgaga
     2821 ggataccgca tattcaatcc ggctcttcag agcaagaaat tcgacccgga aggccgatat
     2881 atccgagagt atgttccaga attggcgaat gtcatgaacc ggtatattca cgagccacat
     2941 ctgatgtccg acgaggaaca ggcacgtgtc gactgccgaa tcggcgtcga ctatcccgag
     3001 cggatcgtgg atcaccggca ggcccgtgaa gactatctcg cgcgaggaaa agctgaggcc
     3061 atgaaatgac cggccaaatg ctccgcctgg gaatcagccg atgtttgttg ggagatgaag
     3121 tccgctttga cggaggtcac aagcacgacc atttcctcac cgacgtgctc ggccgttacg
     3181 tcgaatgggt gccagtctgt cctgaagtag aagctggcct gggaactccc agggaggcca
     3241 tgcggctggt cggcgatccg gctcgtccgc aattgatcac ggtcaagagc gggattgatc
     3301 ataccgaagc gttagagcga atgtcggcga accgtctcca gaatctgaag aggttgagtc
     3361 tttccggcta tatcttcaag aaagattcac cgagctgcgg catcgaacgg gtgcggctgt
     3421 acaaccgatc tggcatgccg agccggcagg gcgtcgggat cttcgcgcgc gcctttatga
     3481 atcagttccc cttgatcccc gttgaggaag aaggacgact gtgcgatcca gacttgcgtg
     3541 aaaattttat cgagcgggtg ttctcctacc gacggtatca ggaccttcta gtgaagagcg
     3601 cctccaggca gagtgtcgtg cagtttcaca cgatgcataa atatttgctc ctatcccata
     3661 gccagcctca ttatgaggca ctgggccggc tcgtcggtca agcgcaccgc tatcgtccga
     3721 aagagttagc cgtgaaatat ggcgagctct tcatgaaggc gctggctgtg aaggccacgg
     3781 tgcggaaaca cgtgaatgtg cttcaacaca tcgttggatt ctttaagact cggctgacga
     3841 cccaagaaaa acgcgagttg ctcggcgtca tcgacgacta ccaccgaggg ctcacaccgc
     3901 tcattgcgcc gctgacactt atcaggcatt acgtgacgat cttcgatgtg agctacattc
     3961 acgcgcaggt ctacctcaac ccgcatccga aggaattgat gttacgcaac caggtctagg
     4021 gggacccaat ggtgacaggt aagaaaggcc ttgtgctcgt cggtcacggg ggaatcccta
     4081 aggactgtcc gcagg
//
LOCUS       DASTQY010000334         7268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1755242, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000334 DASTQY010000000
VERSION     DASTQY010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755242"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VFR82_10975"
     CDS             <1..1143
                     /locus_tag="VFR82_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502165.1"
                     /translation="TRADLVFTLSSDTTNSAWETDDPLHAFGKKEAPFRAAASQYQIL
                     LARYERWLGMPFDDWGFGRKVVPLGKHILAVHRLYVADGFSVGTMQGLERLEKELQSW
                     RTVLREAKTIDTKVLAQILITDDLQLLSRMLSKPVVDKAILAMGLQLTLPLSAAEYSL
                     RWPVRHQVALAVKDGSPGSRSDLQQTQRRSDHDWLTEAAHLPVDAFKSIEHPPARSTM
                     GVFQFGQASNVYAAYYETIIMASESHSQYLPRMQEVVGTLDRGRIESMLAPRLIEPNW
                     ETFHGELIETDTRLRLASLQIQLRRPSSQTAVPTRLAEVGSHYFDPFTGLPMLWSPTQ
                     QKLYSVGKDRLDDGGDPTFDISVPAAVAPTIVKATVKPATTHSTRR"
     gene            1266..1457
                     /gene="rpmB"
                     /locus_tag="VFR82_10980"
     CDS             1266..1457
                     /gene="rpmB"
                     /locus_tag="VFR82_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU4502166.1"
                     /translation="MAFVCDICSKKHQSGHNVSHANNKTKRTFNPNLQRVRALIDGSA
                     RRIRVCTRCLRSGKVKKAL"
     gene            complement(1536..1850)
                     /locus_tag="VFR82_10985"
     CDS             complement(1536..1850)
                     /locus_tag="VFR82_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502167.1"
                     /translation="MARQPLILLLITTFIGSLVGGMLGEWLYLSGEVHAQKTNSVNAE
                     EFLLIDQNGRARAGLGLDPNGEVGLVLSSRDGNRTLALSPDGPVAVKLTDRSGRILWS
                     AP"
     gene            1978..2052
                     /locus_tag="VFR82_10990"
     tRNA            1978..2052
                     /locus_tag="VFR82_10990"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2010..2012,aa:Gly,seq:ccc)
     gene            complement(2388..4145)
                     /locus_tag="VFR82_10995"
     CDS             complement(2388..4145)
                     /locus_tag="VFR82_10995"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEU4502168.1"
                     /translation="MRRNVPHRETARLVQRLAFGVCLLFLFPDPVSLAQTITSSGLNT
                     AVSGPISLPNGQNQVDITGGTRPGGGGNLFHSFGHFSVPSNTTANFLNNSGIPTSNIL
                     ARVTGGNVSNILGTIQTTGFGNANLFLMNPAGFLFGPNATLNVGGMVAFTSADYLRLA
                     DGGRFNANANTGSADILTAAPVAAFGFLGSNPAAISLQGSQLAVAEGTGISLVAGDIT
                     AQGVTLTAPSGQINLVGVKQLSNSNVPGEVLVNGSGQGATFTPIGFATSGLISLSQGS
                     TIDVTGLLGSGSEQAPGKVVIRGGQFVMDASSIRANAYSDAFGPPPGTIEVTVDHALL
                     EHSVIRASAGTEHHGGNIMFNVDTLSATNSFILANGVSRGDGPGGTVEIQAGTRIDLV
                     ATEVNAGTLTAQGGTITMASPLISMNGSAVNVSGGGLDGGPGGTINLVATQTVNLINA
                     RLDAGSGNESRLVPAGAILINGGELFTAQQSVISAEATLGGSILVQACNIALTDSQLI
                     TSTGGANPFTTAGKIALGATKNITLTNGQILSRAYGGSGGTIDITTPILRRNASSVID
                     ASSTLGTAGTVTINGVIQP"
     gene            complement(4959..5339)
                     /locus_tag="VFR82_11000"
     CDS             complement(4959..5339)
                     /locus_tag="VFR82_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4502169.1"
                     /translation="MANASQIRVLIADDHTMVRQALRNALQAFPNIEVVGEAGDGDEA
                     VVATAKLQPKVVVMDINMAKMDGITATRLIKAQHQEIAVIGLSVDLKDYQFHALKNAG
                     ASVVLSKENAALYDLYGAIQQAAA"
     gene            complement(5512..6723)
                     /gene="tyrS"
                     /locus_tag="VFR82_11005"
     CDS             complement(5512..6723)
                     /gene="tyrS"
                     /locus_tag="VFR82_11005"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU4502170.1"
                     /translation="MNELSHQLDLILRGVVEVIQQSELESKVTRSIKEHRPLRVKAGF
                     DPTAPDLHLGHTVLIHKLKHFQDLGHEVIFLIGDFTGTIGDPTGVSETRKVLTKAQVE
                     DNAKTYQRQIFKILDPQKTTIEFNSRWMGPMKADGLIELAAHYRVARMMERDDFQKRY
                     QEQKPISVHEFLYPLVQGYDSVALKADVELGGTDQKFNLLVGRDLQRDYGQEPQVVIT
                     MPLLEGTDGVKKMSKSLGNYVALEDVPSDMFGKIMSISDDLMIRYYELLTTENLSDIK
                     RTHPMEAKQRLASLVVERYHGEQAARQAGEGFQQRFQDRGFPSEPDMRLTLTKADLNT
                     DGAIALTDLIAKTGLVPSKSEARRLVIQGGLEVDGEKQTDANTVLRPIAGRAYQVKIG
                     RRKFALIQLQE"
     gene            complement(6720..7184)
                     /locus_tag="VFR82_11010"
     CDS             complement(6720..7184)
                     /locus_tag="VFR82_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU4502171.1"
                     /translation="MLFSMVIRLAMVAATAGVVFWIGWTLPSSRFLNSDEISLSDRLD
                     TNVHGSTDPLPPSQTGFEAVSKGPQTRSSRPSVSRTVDLNLASKRELEGLPGIGPILA
                     LRIVEYREARGAFQDVEQLRRVKGIGKKTFDRIRALVGVTSSLTMQPNRKAA"
BASE COUNT         1562 a         2054 c         1954 g         1698 t
ORIGIN      
        1 accagagcag acctcgtgtt cactctctca tctgatacga ccaactccgc atgggaaacc
       61 gacgatccgt tgcatgcgtt cggaaagaaa gaggctccct tccgagcggc agccagccaa
      121 tatcagattc ttctcgcgcg atatgagcgc tggctcggca tgccgttcga tgattggggt
      181 ttcggacgca aggtcgttcc actcggcaag cacattctgg ccgttcaccg gctgtatgtg
      241 gccgacgggt tttccgtcgg aacgatgcag ggtctcgagc ggctggaaaa agaactgcag
      301 tcatggcgca cggtccttcg tgaggcaaaa accatcgata cgaaggtgtt ggcgcagatt
      361 ctcatcaccg acgatttgca attgctgtcg cgaatgttat cgaaaccggt tgtcgacaag
      421 gccatccttg ccatgggctt gcaactcacg cttccgctat ccgccgccga gtattcgctc
      481 cgctggcccg tcagacacca agtggccctg gccgtgaagg atggttctcc gggaagcagg
      541 agcgatctcc agcagacaca gcgaaggtca gaccatgatt ggctcacaga ggcggcgcat
      601 cttccggtag atgcattcaa gagcatcgaa cacccaccgg cgcgttccac aatgggcgtc
      661 tttcaatttg gacaggcctc aaatgtgtat gccgcctatt acgaaaccat catcatggct
      721 tccgagagcc actcacaata tttgccacgg atgcaggaag tggtcgggac gctggatcgc
      781 ggacgaattg agagcatgct cgctccacgt ctgattgaac cgaactggga gacctttcat
      841 ggtgaactga tcgaaaccga tacgcgcctc cgcctggctt ctctgcagat ccaacttcgg
      901 cgaccgagct cgcaaacggc ggtccccacg cggttggcgg aagtcggttc acactatttc
      961 gatccgttta caggattgcc aatgctatgg agtccgactc agcagaaact gtatagcgta
     1021 gggaaagacc gcctcgatga cggtggcgat ccgacgttcg acatcagcgt tcccgccgcc
     1081 gtagcaccaa cgatcgtgaa agccactgtg aagccggcga ccacacactc cacacgtcga
     1141 taagtgtcca ttcccgactg ccgaacgcga gattctaaag gctttgaggg agcagtcaga
     1201 cttgtcaatg ctcgctaccg tatgctacag tcacgcgcaa tcacacttat gtgaaggagt
     1261 ctcccgtggc attcgtatgc gatatctgct caaaaaagca tcagtcgggg cacaacgtca
     1321 gtcacgccaa taataagaca aaacggacgt tcaaccctaa tctgcagcgt gtccgcgctc
     1381 tgattgacgg atcagccagg cgtatccgtg tttgcacccg ctgcctccga agcggaaaag
     1441 tcaaaaaagc tctctagcag actcccacac atctccgtta gcaggaaccc tgcgctacgc
     1501 aggcgaccta cttgtctgtc acaacgctgc cgtctttacg gcgccgacca caagatacgt
     1561 cccgaccggt cagtgagctt gacggcaacc ggcccatcgg gggacaaggc caacgttctg
     1621 ttgccgtccc ggctgctcaa gacgagtccg acctccccgt tcgggtccaa acccaagcct
     1681 gcccgcgctc gtccgttttg atcgatgagc aggaactctt cggcgttcac gctgttcgtt
     1741 ttctgggcgt gcacctctcc agacagatag agccactcac ccagcatccc gccaacgagg
     1801 ctgccaatga atgtagtgat cagcagcaga atcagcggct gtctcgccat gataattcct
     1861 ttcttgcacc cttcgaacgc atggtagtgc acttcaattt gggaacgcct ctattgggga
     1921 aacgtgttcc ttgacttgcc gctggagcca tcgtaggatt ctttcctcaa ttcccgagcg
     1981 ggcgtagctc agtggtagag tcctagcttc ccaagctagt tgtcgtgggt tcaaatccca
     2041 tcgcccgctc caaccaagct tcctcgtgcc ggcgcctcat cgagttagcc ctctgctttg
     2101 ggacaccggt agaaccgtgc agtgtgcgtc ttgttcctct atgcgaaccg gcggctcctc
     2161 aggcgaggcc tctcttcatg gtcgccgccg gaataacacc acctgtgtct atcctctcga
     2221 agcggcgcct tgctcctcta catagaaatc aactttacat tctcctgatc tgcacggcag
     2281 tcaaacggat cgcgtcgaag ataccatctc tcacggattt atttcagcat ctgtatgaga
     2341 ccctcgtcgg ggagcatggt ccatcagggc gttggaaccg actgtcccta aggttgaatg
     2401 accccgttaa tggtgaccgt gccggctgtg ccgagcgtgg aggaagcgtc tatcacgctg
     2461 ctggcattcc gacggaggat aggggtcgtg atgtcaatgg tgccgcccga cccaccatac
     2521 gccctgctaa gaatctgacc gttcgtcagc gtaatgttct tcgtcgcacc cagggcaatc
     2581 ttgcctgctg ttgtaaatgg gttggcgcca ccggtagagg tgatcaactg gctgtcagtg
     2641 agggcgatat tgcaggcctg caccaggatg ctgccgccga gtgtcgcttc tgctgatatg
     2701 acgctctgtt gagcggtaaa cagctctccg ccattgatga gaatggcacc agctggaacg
     2761 aggcggctct cgtttccact gcctgcgtcg agtctggcat tgatgagatt cacggtctgt
     2821 gttgccacca ggttgatcgt gccaccagga ccaccgtcca atccgcctcc cgacacgttg
     2881 accgcactac cattcatcga gataaggggc gatgccatgg taatcgttcc accttgagct
     2941 gtgagagtgc ccgcattcac ttccgtagcc acgaggtcta ttctggtccc tgcttgtatc
     3001 tcaaccgttc cgcctggtcc gtcccctctg gaaactccat tcgctaagat gaaggaattg
     3061 gtggcagaca aggtatcgac attgaacatg atgtttccgc catgatgctc ggtccctgcg
     3121 gaagccctga tgacgctatg ctccaataac gcgtgatcga ccgttacttc tatggtaccg
     3181 ggtggagggc caaaggcatc actgtatgcg ttggcccgaa tagaggaagc atccataacg
     3241 aattgtcctc cacgaatcac caccttgccg ggcgcttgct cactaccgct tcccaacaat
     3301 cccgtcacgt caatggtgct gccttgagag aggctgatga ggcctgaagt ggcgaatcca
     3361 atcggagtga atgtcgcccc ctgtcccgat ccatttacga ggacttctcc tgggacgttc
     3421 gagttcgata actgtttgac tccgaccagg ttgatctgcc cactcggagc cgtcagtgtg
     3481 acaccttggg ctgtgatatc gcccgccacc agcgagatcc ctgttccttc tgcgacggct
     3541 aactgactgc cctgaagtga aatggcagct ggatttgagc ctagaaagcc gaatgccgct
     3601 acgggcgcgg cggtgagaat atcggccgag ccagtgttcg cattcgcatt gaagcgtcct
     3661 ccgtctgcaa gccgtaagta gtcggcgctg gtgaaggcca ccatcccacc aacattcagg
     3721 gttgcattgg gaccaaacag aaatccagca ggattcatca ggaagaggtt cgcattcccg
     3781 aatcccgtgg tttggatggt gcccagaatg ttggagacat tgcctcccgt cactcgcgcg
     3841 aggatgttag atgtgggaat accggagtta ttcagaaagt ttgcggttgt attgctgggt
     3901 acactgaagt gaccgaaact gtgaaataaa ttcccgccgc caccgggtcg cgtcccgcct
     3961 gtgatgtcga cctggttttg gccgttaggg agagaaatcg ggccggacac tgcggtattc
     4021 agtccagaag atgtaatcgt ttgcgccagg gaaaccgggt cagggaatag aaaaaggaga
     4081 cagacaccga atgcgagacg ctgaaccaac ctggcagttt cacgatgggg tacgttgcgg
     4141 cgcatgtttg acctccccga cgctgttggg caccctgctg gcgtgatgcc agcattcatc
     4201 atgaggatgc cggttggacc gactgtgtag cttcgtgctt tggtactgac gaaacacgat
     4261 cgaaacggcg gtattggcag gatatggaat gggttttgtg taaacgaaag atctgccaga
     4321 ggcgtcgcct ataggcgacg tgcgttgact cgccgcccct tcttcgtagt gcaatcgcac
     4381 tcagagtcgt gcctcatttg agacacgaga gggtttctgg ccggtggcac gtccgtgcga
     4441 gccgagtgaa ggccagaaca tgcggtcgct tggtggaaaa tccatactgt tacctgcccc
     4501 tggatccctc taggccaaag tgactctatt cgcccacctc ccaaggcaag gcttatgccc
     4561 tttcttgctt cttgaatacc gcgaagaagc aagtatttat cctagacgag gttggttagt
     4621 gggatacctt gtccgcaaat gagggaagaa ctgtctctcc cgacagtgat tcgtcagaat
     4681 ttctcgtaat tggattcgct gtgtcaggac aagtcgtccg actcgctgaa gccgaagctc
     4741 gcttagggca ctcggagcat agataggttg aaagagggga aggaattaca agaggagtag
     4801 aggagatcct agacttcggg cttcacgttt gaggcatcac ctcatgaaac accttgagtc
     4861 ccagcattca ggaattatcc gagcgacgga agcatacctc gagcagcgag aaacctttcc
     4921 gatcggacca tatgagattc agcggcgtaa tcgcgagttc acgctgcggc ctgttggatc
     4981 gcaccgtaca agtcgtacaa ggcggcgttc tccttgctga ggaccactga ggctccggca
     5041 ttcttcagcg cgtgaaactg gtagtctttc aaatcgacgg agagtccaat cactgcaatc
     5101 tcttgatgct gggccttgat taaacgagtc gccgtaatac catccatctt ggccatgttg
     5161 atatccatca caacaacctt cggctgaagc tttgccgtag cgacaaccgc ttcgtccccg
     5221 tcacctgctt ctccaaccac ttcgatattg ggaaatgctt gcaacgcatt gcgtaacgct
     5281 tggcgaacca tggtgtgatc atcagctatg agcactcgaa tttgactggc attagccata
     5341 aagtccccta taagaatgaa aataatatgg ataaagagtt tagaagacgc ttatccgtgg
     5401 gtttcgccaa ctaggacctt ccccagtctt gatctgcgcc gtgggcatac gcgtcgttat
     5461 tcatcataaa ggagctgagc cattttactg aacgcgctgg caggttgctc tttattcctg
     5521 caattggatt agagcaaatt ttcttcggcc gatcttcacc tggtaggcgc gtccggcgat
     5581 cggtctcaac acggtgttcg catcggtctg cttctctccg tccacttcca atcctccctg
     5641 tatgaccagc cgccgtgcct cgctcttgct aggcacaagc cccgtcttgg caatgagatc
     5701 ggtaagggcg atggcgccgt cggtgttgag atcggccttg gtcagtgtga gtcgcatatc
     5761 cggttcgctc gggaagcctc tatcctggaa cctctgttgg aagccctcgc ccgcctgacg
     5821 tgccgcctgc tcgccgtgat agcgctcaac gacgaggctg gccaatcgct gcttggcttc
     5881 catgggatgc gtccgtttaa tgtcggacag attttcagtg gtcagcaatt cgtagtacct
     5941 gatcatgaga tcgtcgctga tggacatgat tttgccgaac atgtcacttg gaacatcctc
     6001 gagcgccacg taattcccga ggctcttgct cattttcttg acgccatcgg tcccctcaag
     6061 caaaggcatg gtgatgacga cttggggttc ctgtccataa tctctctgca ggtcacgtcc
     6121 caccaacagg ttgaactttt gatcggttcc gcccaattcc acgtcggcct ttagtgcgac
     6181 ggagtcatag ccctgtacca gcggatagag gaactcatgg acactgattg gcttctgctc
     6241 ctggtatcgc ttctgaaaat catcgcgctc catcatccgc gcgacgcgat aatgggcggc
     6301 aagttcgatg aggccgtccg ccttcattgg tcccatccat cggctgttga attcaatggt
     6361 ggtcttctgt gggtccaaaa tcttgaaaat ttgccgttgg taggtcttgg cgttgtcctc
     6421 cacttgtgcc ttggtaagga ccttccgcgt ttcggagacc cctgtcggat cgccgatcgt
     6481 gcccgtaaag tcgccgatca agaaaatgac ttcatggcca agatcctgga aatgtttgag
     6541 tttgtggata agaacggtat ggccgaggtg taagtcggga gccgtcggat caaacccggc
     6601 tttcacccgc aacggtcggt gctccttgat cgagcgcgtc accttggatt ccagctcgga
     6661 ttgctgaatc acctcgacca ctccgcggag gatcagatcc agttgatggg acagctcgtt
     6721 catgccgcct tcctattggg ttgcatggtc agtgacgagg tcacaccaac caaggcacgg
     6781 atgcggtcga atgtcttctt cccgattccc tttacacgac gcagttgttc tacatcttgg
     6841 aaggcacctc gtgcttcccg atactcgaca attcgcagcg ccagaatcgg cccgatgcct
     6901 ggcagccctt caagctcgcg cttagacgct agattcaaat cgacggttcg ggacactgat
     6961 gggcgtgacg atcgggtttg cggcccttta ctgactgcct caaatcctgt ttgggatggc
     7021 ggcaggggat ctgtggaacc atgcacgttc gtatcgagcc tgtctgatag cgagatctcg
     7081 tccgaattga gaaaccgcga tgagggaagc gtccatccga tccagaagac gacgccggcg
     7141 gttgcggcaa ccatagccag cctgatgacc attgagaaca gcatctacaa tccttctttg
     7201 caactaaccg gttccggttc cgttcccgtc ctctgctgcc tccattaagc cttcggtgga
     7261 cgcatgga
//
LOCUS       DASTQY010000335         4873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_836330, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000335 DASTQY010000000
VERSION     DASTQY010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836330"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..513)
                     /locus_tag="VFR82_11015"
     CDS             complement(208..513)
                     /locus_tag="VFR82_11015"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU4502172.1"
                     /translation="MGHERESGAATVLAFLGGAAVGAVAALLLAPQSGRTTREQLRGY
                     ARKAEDALREAANEAGQRLEEAVNEGKEYVDARKSVLREAFEAGREAMKREQTRGES"
     gene            complement(503..910)
                     /locus_tag="VFR82_11020"
     CDS             complement(503..910)
                     /locus_tag="VFR82_11020"
                     /inference="COORDINATES: protein motif:HMM:NF017876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU4502173.1"
                     /translation="MTLIELAACLAAAAFAVLVGFLVPVLINVRKTVAESERLVTKLN
                     ADLPALTSDLRSVTQNLNALADQARNGVEHASVLLHAVGEVGESVQEVHNVVRGSSGT
                     LLTNVASLVAGVKAATRVMRNRYRQEGEPHNGA"
     gene            complement(953..2419)
                     /gene="gatA"
                     /locus_tag="VFR82_11025"
     CDS             complement(953..2419)
                     /gene="gatA"
                     /locus_tag="VFR82_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU4502174.1"
                     /translation="MSLHKLSLCELQKKFTAGEVTATEIVRAYFLRIGQVEPKVKAYV
                     TQTKEAAIARASALDERLKGWRKTKPMTGMPLAIKDNLCTEGVRTTCSSRMLQQFIPP
                     YDATVIAKLRDQDYILLGKANLDEFAMGSSTEHSAFGPSRNPWNLQHVPGGSSGGAAA
                     AVAADECAAALGSDTGGSIRQPAAFCGVVGLKPTYGRVSRYGLVAFASSLDQVGPITK
                     DVPDAALLLGAIAGHDPLDSTSADLPVPDYMKALKKKDLKKLTVGVPVEYFADGLDPE
                     VELSVRAAIDEMKSLGGTIKEIHLPRTDAAVAVYYVIATAEASSNLARYDGVKFGLRA
                     KETKDLLDLYMKTRQEGFGPEVKRRIMLGTYALSAGYYDAYYGKAQAVRTLICEDFRE
                     AFKDVDLIVTPVTPTPAFRLGEKSEDPLQMYLSDIYTISVNLAGLPAIAVPCGFSKAG
                     LPIGVQLIGRAFEEETLLRAAHAYEQSTQWHLKKAIVR"
     gene            complement(2434..2778)
                     /gene="panD"
                     /locus_tag="VFR82_11030"
     CDS             complement(2434..2778)
                     /gene="panD"
                     /locus_tag="VFR82_11030"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEU4502175.1"
                     /translation="MLRSKIHRATVTGAHLEYEGSLTIDEDLMEAAAILPYEAIVCSN
                     LNNGERFMTYAIGGRRGSGDIVLNGPTARKAAVGDQIIIFCYEYYGDEEIKKHAPRIV
                     RVNEKNQIVESR"
     gene            complement(2823..3116)
                     /gene="gatC"
                     /locus_tag="VFR82_11035"
     CDS             complement(2823..3116)
                     /gene="gatC"
                     /locus_tag="VFR82_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU4502176.1"
                     /translation="MKITLEDVEKVAKLARLEVSPAEKDAFAKQLSQILTHVDKLREY
                     DTQGVEPTAAVLGQVNVFREDIVRPSLPVDKALANVPEREGDGFAVPKIIEDR"
     gene            complement(3347..>4873)
                     /gene="glmS"
                     /locus_tag="VFR82_11040"
     CDS             complement(3347..>4873)
                     /gene="glmS"
                     /locus_tag="VFR82_11040"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU4502177.1"
                     /translation="ENYQPLKQQLQREGYAFQSETDTEVVAHLIDKHLQRGLNLADAV
                     RAATKEVRGSYALAVICEKEPERLIAARSGCPLVVGRTKQASYVASDVMAMLAHTRDV
                     IYLEEGDVASVTQSEVQVIDAEGREVSRKTSRVTWDASAAEKSGYPHFMLKEIHEQPQ
                     TILDTMRGRYSYETGEADLADIGLTPSEFLAVDRIWIVACGTSWHAGLVGKYLLEEMV
                     RTPVQVDIGSEFRYRDPLVGKHDLFITISQSGETADTLAAAREAKEKGARVLSIVNVV
                     GSTLARESDGVLYTHCGPEIGVASTKAFTAQLTALYLLALHLARIRNVMNVADGKAWL
                     DRLVRLPSLVEGVLGREAEIVAIAKRYYKKRNFLFLGRGINYPIALEGSLKLKEVSYI
                     HAEGYAAGEMKHGPIALIDRDMPVVVLAPRDRLYEKTVSNLMEVKARRAPVIALVAEG
                     ERELGRTADAVFTIPDTHPLMSPILFTIPLQLLAYHIAVLRGADVDQPRNLAKSVTVE
                     "
BASE COUNT          972 a         1545 c         1287 g         1069 t
ORIGIN      
        1 tgggcatgga cttcgacacc gatcacgact tcgtagacgg cttgactatg actctgctgc
       61 agaatcatcg cagctccatt cgtatagacg ggcgtgtggc acaatatctc tcacgaagtc
      121 cggcgagtaa aggagagaag aaggagctat cctgcttgcc gtgatccctg caagcaggta
      181 cgtgtctcca gagacttgag ggcgccctta gctttcgccc cgcgtctgct ctcgcttcat
      241 cgcctcccga ccggcttcaa acgcctcacg caacaccgac ttcctcgcat ccacgtactc
      301 tttgccttca ttgaccgctt cttcaagccg ttgcccggcc tcgttggcgg cttcgcgcaa
      361 ggcgtcttcc gctttgcgcg catagccgcg cagctgctcc cgtgtggtgc gtcccgattg
      421 tggcgccaac agcaatgcgg caaccgctcc cactgccgcg ccccccagaa acgccaaaac
      481 tgtcgctgct cccgattccc gttcatgccc cattgtgtgg ttctccttcc tgacgataac
      541 gattacgcat cacccgggtc gcagccttga cgcctgcgac aagactcgca acattcgtca
      601 acagcgtgcc gctcgaacct cgcacgacat tgtgaacttc ctgcaccgac tcacccacct
      661 caccgaccgc atgcagcagc acagacgcat gctcgacgcc attcctggcc tgatcagcca
      721 atgcgttcaa gttttgggtg acggaacgaa gatccgaagt cagcgccgga agatccgcat
      781 tcagcttcgt caccagccgt tcggactcgg caaccgtttt acgtacgttg atcagcaccg
      841 gtaccaagaa accgaccagg acggcaaagg ccgcagcagc aagacaagca gccagttcaa
      901 tgagcgtcat accaacctcc gggctacagg cgagaagggc gttccgcgtg tttcatcgga
      961 caatggcttt cttgaggtgc cactgtgtgg actgctcgta ggcatgcgca gccctgagca
     1021 gcgtctcctc ctcaaaggcg cgcccgatca actgtacgcc gatgggaaga cccgccttgc
     1081 tgaatccgca tggcactgca atcgccggca atcccgcaag gttgaccgag atggtgtaaa
     1141 tgtccgacag atacatttgc agaggatctt cgctcttctc gcccagtctg aacgccggcg
     1201 tcggtgtgac cggcgtcaca atgagatcga cgtccttgaa ggcttcgcga aaatcctcgc
     1261 agatcagcgt tctgacagcc tgcgcttttc cgtaataggc gtcgtaatac ccggcgctta
     1321 aagcataggt gcccagcatg atgcggcgtt ttacttccgg tccgaaccct tcttgtctgg
     1381 ttttcatgta caggtcgagg aggtccttgg tttcctttgc acgcaggccg aatttcacac
     1441 cgtcatagcg tgccaggttc gaactggcct cggcggtagc gatcacgtaa tagacagcta
     1501 cggcggcatc cgtcctgggc aaatgaatct ccttgatcgt cccacccagg cttttcattt
     1561 cgtcgatcgc cgcacgaacg gacaactcga cttccggatc caggccgtcg gcgaaatatt
     1621 ccaccggcac accgacagtc aactttttga gatccttttt tttcagcgct ttcatgtaat
     1681 cgggaacggg gaggtcagca gaggtggaat ccaacgggtc gtgaccggcg atggcgccca
     1741 gcaagagcgc tgcatccggc acatctttgg tgatcggacc gacctgatcc aatgatgagg
     1801 caaacgccac gagaccataa cgcgataccc gtccatacgt gggcttcaga cccacaacgc
     1861 cgcagaacgc cgccggctgg cggatcgagc cgccggtatc tgagccgagc gccgccgcgc
     1921 actcatccgc cgccacggct gcggccgcgc ctccgctcga gccgccgggc acgtgctgga
     1981 gattccaggg gttccggctt ggaccgaagg cggagtgttc ggtggatgat cccatggcaa
     2041 attcatcgag gttcgccttg cccaagagga tatagtcttg gtcccgcaat ttggcgataa
     2101 ccgttgcatc atacggcggg atgaactgtt gcaacatccg ggacgagcaa gtcgtccgca
     2161 ctccctccgt acagagattg tccttaatgg ccaggggcat acccgtcatc ggcttggttt
     2221 tgcgccaccc cttcaagcgc tcgtccagtg cgctggcccg cgctatggcg gcttccttgg
     2281 tttgtgttac ataggctttg actttgggtt cgacctgtcc gatccggagg aaataagcgc
     2341 gcacgatttc cgtggccgtg acctcaccgg cagtaaattt cttctggagt tcacagagtg
     2401 aaagcttgtg cagagacatg accgtaatct cgattatctc gattcgacga tctgattctt
     2461 ttcgttaacg cgcacgatcc tcggcgcatg ttttttgatt tcctcatcgc cgtaatactc
     2521 gtaacaaaag atgatgatct ggtcccccac ggccgctttg cgcgccgtcg gcccgttcaa
     2581 cacgatatcg ccgctgcccc gacgtccgcc gatcgcatag gtcatgaagc gctcgccgtt
     2641 gttcagattc gagcatacaa tcgcctcata cgggaggatc gcggccgcct ccatgaggtc
     2701 ctcgtcgatg gttaaactgc cttcgtactc gagatgcgcg ccggtcactg tggcgcggtg
     2761 aatttttgat cgcaacattt gccggaacat cgttacttca ccttacctct cttgcgatat
     2821 cgttaccgat cctcaataat tttcggcacc gcgaacccgt ccccttcccg ttccggtacg
     2881 ttggccaagg ccttgtcaac aggcaacgac ggccggacga tgtcctcccg gaatacgttc
     2941 acctgcccaa gtacagcagc ggtcggttca acaccctgtg tgtcgtactc tctgagcttg
     3001 tcgacgtgcg tcaatatctg gctgagttgt ttggcaaagg cgtccttctc agctggggac
     3061 acctccagcc gcgccaactt cgccaccttc tcaacatcct ccagtgtgat cttcatcact
     3121 caatcattcc atagcgaagg ttcaaaatgg ctttccagca aggccgcagg aagtccggcg
     3181 gccgagacct atccttctca cccaccgccc cgagctgcca agacagctcg ttcaccggtg
     3241 ggtacgtcgc aggacgacgg acaaccgaga acgaagctgg aagccatttt cagcacgtct
     3301 tcttctcacc gcccaccccg gcgtcttaga agcacgccct tcccggctat tccaccgtca
     3361 cgctcttggc aagatttctc ggctgatcca cgtccgcccc ccgcagcacc gcaatatgat
     3421 aggcgaggag ctgcagagga atggtgaata agatcggcga catcaaggga tgcgtgtcgg
     3481 ggatggtaaa taccgcatca gccgtcctgc ctaattctcg ctccccttcg gccacgagcg
     3541 caatgaccgg tgctcgtcgc gccttgacct ccattaggtt gctcacagtc ttttcataca
     3601 ggcgatctcg cggtgccaac acgaccaccg gcatgtcccg gtcaatcagc gcgatcggcc
     3661 catgtttcat ctcaccggcc gcatatccct ccgcatgaat ataagacact tccttcaatt
     3721 tgagagaacc ttcgagcgcg atgggataat tgatgccgcg tccgagaaac agaaagttcc
     3781 gctttttata atagcgtttg gcgatcgcga cgatttccgc ttcgcgtccc aacactcctt
     3841 ccaccagcga gggcagccgc acgagccggt ccagccatgc cttcccgtcc gccacattca
     3901 tcacattgcg gatgcgggcc aggtggagcg ccagcagata gagtgcggtg agttgggccg
     3961 taaatgcctt agtagacgcg acaccgatct ccggcccgca atgggtgtag agcactccgt
     4021 cagactcgcg ggccaatgtg ctgccgacga cattgacgat cgacagaacc cgagcgcctt
     4081 tttccttggc ctcccgcgcc gcggcgagcg tgtcggctgt ttcgccggat tgggaaatcg
     4141 tgatgaagag atcgtgcttc cccacgagcg gatcgcggta ccgaaactca cttccgatgt
     4201 caacctgcac cggcgtccgg accatttcct ccaataaata tttgcccacc agtcccgcgt
     4261 gccacgacgt cccgcaggcg acgatccaga ttcgatccac ggccagaaac tcgcttggcg
     4321 tcagaccgat gtcggcgagg tccgcttcac cggtttcata cgaatagcgg ccgcgcatcg
     4381 tatcgagaat cgtctgcggc tgctcgtgga tctctttcaa catgaaatga ggatacccgc
     4441 ttttctcagc tgccgaggcg tcccatgtga cgcgtgaagt cttccgcgag acctctcgac
     4501 cttcagcgtc gattacctgc acctcggatt gggtcacgct cgccacgtcg ccttcttcga
     4561 gatagataac gtcccgggta tgcgccagca tcgccatgac atccgatgcc acgtaagatg
     4621 cctgcttcgt gcgccccacg acgagcggac agccggagcg cgctgcaatg agccgttccg
     4681 gctctttttc gcagatcacg gccagcgcgt agctgccgcg cacttccttg gtggctgcac
     4741 ggacggcatc ggccaagttc aaaccgcgtt ggagatgttt atcgatgagg tgggccacga
     4801 cctcggtgtc ggtttctgac tggaaggcat agccctctct ttgcaactgc tgcttgagcg
     4861 gttgataatt ttc
//
LOCUS       DASTQY010000336         3339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1038674, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000336 DASTQY010000000
VERSION     DASTQY010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038674"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFR82_11045"
     CDS             complement(<1..287)
                     /locus_tag="VFR82_11045"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEU4502178.1"
                     /translation="MRAILAQCTLACLLLLVDQTAMATMATDVATPPTQAVHTGERLT
                     YDISWLNVLAGTAVMEVQPDGTGSRARLVTTAQSRPAISKFFPVDNRVESE"
     gene            complement(327..1727)
                     /locus_tag="VFR82_11050"
     CDS             complement(327..1727)
                     /locus_tag="VFR82_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEU4502179.1"
                     /translation="MGLFREYDLRGIVGDDLTEAIAEQVGRAYCTYVKARGVQTISVG
                     RDGRLSSPALFKSLVSGLLAGGVNVIDIGICPSPLLYFSLFQLPVQGGIMITGSHNAA
                     EYNGFKVCVGKEAIHGETIMELRRILETASFTSGQGRLSEHTIIPDYLEYLRTSFKGV
                     NAGRLHVVIDCGNGAAALVAKEALEILGCRVTGLYCDLDGRFPNHHPDPTVLENLEDL
                     VNAVKQHKADVGIGYDGDADRIGAIDEQGHVLWGDRLMVVYSRDILRTIPGSTIISEV
                     KASQCLYDDIAKQGGRAIMWKTGHSLIKAKMKSEKAVLAGEMSGHMFFADRYFGYDDA
                     IYASCRLVEILAKTGQSLSSLVADLPATSVTPEIRVDTPDSIKFELVKRVQDRLAGYA
                     KSSRPIGPNNDVVRDVVTIDGVRAIFEDGWGLIRASNTQPALVLRFEASSADRLSAIR
                     AVVEGELAEVKRSMLS"
     gene            complement(1742..3193)
                     /locus_tag="VFR82_11055"
     CDS             complement(1742..3193)
                     /locus_tag="VFR82_11055"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEU4502180.1"
                     /translation="MGDKPTLYPVIMAGGSGTRFWPLSRQLFPKQLLKIIGDETLIQQ
                     TMRRVVKGAPPERVLISTNPAQAESIRVQLSEWKDALKDNFVLEPEGRNTAPAIALVA
                     LELVRRDPEAIMLVVPADHIVKGQRDFDAAVSVAASLALQGLLVTFGIKPIRPETGYG
                     YIKPNMKTVLARQGKLKGYRVGRFVEKPDAVKAAQYLKAGDYYWNSGMFVWRAATILE
                     EIRRHQPVLANGIDRIGRLINQGAAKAAIDEEYRLLPSISIDTGVMERSNKAAVVPVS
                     FSWSDVGSWGSLDEVAAKDKAGNVVGGRVVDIDSQRSVVYGDRRVVATIGLVDMVVVD
                     TPDATLVCPKSRAQDVKKIVDILKQQGAPEHLEHLTVHRPWGSYTVLEEGPGYKVKRV
                     TVHAGGRLSLQLHHKRSEHWVVIAGTARVTRGEEVFELHVGQSTAIPVETRHRLENFG
                     QDTLHIIEVQNGPYLGEDDIVRFKDDYGRTAAS"
BASE COUNT          686 a         1020 c          904 g          729 t
ORIGIN      
        1 aactcggatt ccacgcggtt gtcgacagga aaaaacttac taatggccgg ccgtgactga
       61 gcggtcgtga caagtctggc gcgcgagccc gttccatccg gttggacctc catgacggcg
      121 gtccctgcga ggacgttcaa ccaggagatg tcgtaggtga gccgctcacc ggtgtgaacc
      181 gcttgcgtcg gtggagtcgc cacatctgta gccattgtag ccatcgccgt ctggtcgacg
      241 agcaacagca ggcaggccaa ggtacattga gcgagtatgg cacgcatgat aacgatgact
      301 gtctcgggga gatggcagga atggaactag gagagcatgg agcgcttcac ttcggccaat
      361 tcgccctcaa cgacggcccg gattgcggac aagcggtcgg ctgaactggc ttcgaatcgg
      421 aggactaatg ccggctgcgt gttcgaggcc cggatcaagc cccatccgtc ttcaaagatg
      481 gcccgcaccc cgtcgatcgt caccacgtca cggacgacgt cgttgtttgg gccgataggc
      541 cgactggact tcgcatagcc ggctagccgg tcctgcaccc ttttgaccag ttcgaatttg
      601 atcgaatcag gagtatccac tcgaatttcc ggtgtgacgg atgtggcagg cagatctgcg
      661 actaggctgg aaagcgattg gccggtcttc gccaatatct cgacgaggcg gcaggaggca
      721 tagatcgcat catcatagcc aaagtatcgg tcggcaaaaa acatatggcc agacatttct
      781 ccagccagca cggctttctc gctcttcatt ttcgctttaa tgagggaatg accggtcttc
      841 cacatgatcg cgcggccgcc ttgcttcgcg atgtcatcat agaggcattg cgacgccttg
      901 acctcggaaa taattgtgct gcccggtatc gtcctcaaaa tatcccggga atagaccacc
      961 atcagacgat caccccacaa gacgtggcct tgctcgtcaa tggcgccgat tcgatcagcg
     1021 tctccgtcgt agccgatccc gacatccgcc ttgtgctgtt tgactgcgtt gaccaagtct
     1081 tccagatttt ccagcaccgt cggatcagga tgatggttcg gaaaccggcc gtccagatca
     1141 cagtagaggc cggtcacgcg gcagcccaaa atttcgagcg cttctttggc gaccaatgct
     1201 gccgcaccgt tcccgcagtc gatgaccaca tgcagccgcc cggcattgac gcccttgaag
     1261 cttgttcgga ggtactccag atagtcgggt atgatcgtat gctcgctgag ccgcccttga
     1321 ccggacgtga aactcgccgt ttccaggatc cgtcgaagct ccatgatggt ttcgccatgg
     1381 atggcttcct tgcccacaca gaccttaaat ccgttgtact cggcggcatt gtggctgccc
     1441 gtgatcatga tgccgccttg gaccggaagc tggaacagtg aaaaatagag caatggagac
     1501 ggacaaatgc cgatatcgat gacgttcacg ccaccggcta gcaagccgct gaccagggat
     1561 ttgaacaagg cgggagagct cagcctgcca tcgcgaccga cggaaatcgt ctggacacca
     1621 cgtgccttga cgtacgtgca gtaggcgcga ccgacctgtt ccgcaatcgc ctctgtcagg
     1681 tcatccccca caatgcctcg aagatcgtat tctcgaaaca gtcccatcaa cgctccctgt
     1741 gttaactggc agccgtccgc ccataatcat ctttgaaacg cacgatgtca tcttccccaa
     1801 ggtagggacc gttctggact tcaataatgt ggagggtgtc ttgaccgaag ttttcgaggc
     1861 ggtgtctcgt ttctaccgga atggccgtgc tttgccccac gtgcagttcg aacacctcct
     1921 cgcctctcgt cacacgggcc gtccccgcaa tcacgaccca atgctcgctc cgcttatgat
     1981 gcagctgcaa tgacagccga ccacccgcat gcaccgtgac tcgcttgacc ttgtatccgg
     2041 ggccctcttc gagaaccgta tacgatcccc aaggccgatg gaccgtcagg tgctcgagat
     2101 gctcgggggc tccttgctgc ttgagaatat cgacgatctt cttgacgtct tgggcacggg
     2161 acttaggaca gacgagcgtt gcatcggggg tatcaaccac aaccatgtcg actaacccaa
     2221 tcgttgccac gactcgacgg tctccataca ccacggaacg ttggctatcg atgtcgacga
     2281 cgcggccacc caccacgtta ccggccttat ctttcgcggc gacttcgtca aggctgcccc
     2341 aactcccgac gtcggaccaa ctgaacgaca ccggcacgac cgcggccttg ttggaccgct
     2401 ccatcactcc ggtatcaatt gagatggacg gcaacaggcg atactcttca tcgatggcag
     2461 cttttgccgc tccttggttg atcaagcggc cgatccggtc gattccattg gccagtacag
     2521 gctgatgccg gcggatttct tccaaaatcg tggcggctcg ccacacgaac atgccgctgt
     2581 tccaataata atcgccggct ttgaggtact gcgcagcttt aacggcgtcg ggtttttcga
     2641 caaaccggcc gacccgatac cccttcagtt ttccctgacg cgccaggact gtcttcatat
     2701 tcggcttgat atagccgtat ccggtttctg gtcgaatcgg cttgatgccg aaagtgacca
     2761 gcagaccttg gagtgcaaga gaggccgcca cagagaccgc tgcatcaaaa tccctctgtc
     2821 ccttcacaat gtggtcggcc ggcaccacca gcataatcgc ctcgggatct cgacggacca
     2881 gttccagcgc aaccagagca atcgccggcg cagtgttgcg gccttccggc tcgagcacga
     2941 aattgtcttt gagcgcatcc ttccattcac tgagctgcac tcggatcgat tcggcttggg
     3001 ctgggttcgt cgagatcagg acccgttcag gcggcgctcc cttcacgacc cgtcgcatcg
     3061 tttgttgaat cagcgtttcg tcgccgatga tcttcagcaa ctgcttggga aataattggc
     3121 ggctcagcgg ccagaatctg gtcccgctgc cgccggccat gatcaccggg taaagagtag
     3181 gcttgtcgcc catcgctcgc ctcgattgtt tagggggcct tctgctgagc cttgagaatc
     3241 atctcgatga tttcgcgcac cgcgccctca ccgccttttt tgacgcagac ataatccact
     3301 gcttcagcca ccacaggcat cccgtcggcc ggcgtggct
//
LOCUS       DASTQY010000337         7103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_471610, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000337 DASTQY010000000
VERSION     DASTQY010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471610"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /gene="glgP"
                     /locus_tag="VFR82_11060"
     CDS             complement(<1..1182)
                     /gene="glgP"
                     /locus_tag="VFR82_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEU4502181.1"
                     /translation="MNTTSIDPVRTHIPTSLARLPELSQNLWWSWTVEARQLFETIDP
                     TLWFYTHHNPVKLLADIQPERLAKLAEDPSYLRQYSAVLKLFDEYRSNGRSWFTIQHA
                     SETKKVIAYFSAEFGLHTSIPIYSGGLGILAGDHCKEASDLGVPLVGIGFMYPQGYFR
                     QRITAEGWQEAAYAPFNREDSPIHPALTPSGEACRFTVDMGGRNVTAVVWKVLVGRIP
                     LYLIDTDVPENSAENRALSARLYGGDQEMRLCQEILLGIGGVRLLRALDIAPSVWHAN
                     EGHSAFLTLERLRELVQKGLSHAEASDMVRQSTVFTTHTPVPAGHDVFPHYLMDRYFA
                     GYWEQLNLSRDEFLGLGETPESRGHGFNMTALVMRLSAHVNGVSREHGRVSREMWQHF
                     WP"
     gene            complement(1241..2083)
                     /locus_tag="VFR82_11065"
     CDS             complement(1241..2083)
                     /locus_tag="VFR82_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502182.1"
                     /translation="MSTANMSYSEQRRSLADLMLAVAGESVTVLKKNAPEQALKLKRQ
                     DEWKLYLEFLKVTFNLADRLSAFHIPVKDQPDFMSGLEEAVTARLRAVLEPAFGSGVD
                     QNEIMMTISGVVAESRKLYESYRFSITEDSKTKNEMFDAFGGRIADALGVADNPQITS
                     AARLCATAMIPAITAILKGEPPPLADTASQLSGSAASPGAPAQRGQTGNEIKLISVMS
                     DVSGDEIETRWGLHPRFRQDLTAGEIQQLTKLMNRVAKVLGERYAAVAFSEEWTSWHK
                     AGHA"
     gene            2277..3122
                     /locus_tag="VFR82_11070"
     CDS             2277..3122
                     /locus_tag="VFR82_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEU4502183.1"
                     /translation="MENLPLLFVQTVVLRPYVFVFLAAFLLIGGRLLGWRRTGVFFLA
                     SWITAFVCEYSSTRIGVPFGFYYYTGSTVGQELYIADVPFMDSLSFTFLLFSSYCLAL
                     VFLLPSVTRVGGRRLQFVLDPKIRTSWSSLLLTATFLTVIDVIIDPVALRGDRWFLGR
                     IYGYPDPGVYFGIPLMNFVGWAVVGLVGLGIYFYVDRSLPPLSLHSDRSVATTPLLLG
                     CTLYYGVLLFNLVITFWIGEPLLGLVGMMIYLPITIVFVLRLLNRIPTPRLAVGTTST
                     LAPDA"
     gene            3208..5229
                     /locus_tag="VFR82_11075"
     CDS             3208..5229
                     /locus_tag="VFR82_11075"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEU4502184.1"
                     /translation="MSRDITPYILKRQFEDAAPRNLSIDYAAALNPQQLAAVTATDGP
                     SLVIAGAGSGKTRTLVYRVAYLIDCGIDPSNILLLTFTRKSAQEMLQRAGELIGMRSE
                     RVCGGTFHSVANMLLRRYGRGVGLEPGFTILDRSDAEDLIALVRSQLGLNEKDKRFPR
                     KSTIAEIFSKSENTLRSLDDILMEEFSHFADHIDALGQLQTGYRAAKRQRQLVDYDDL
                     LVLLRQLLMQDPAARQSISRLYRYILVDEYQDTNRLQADVIRNLAVSHQNVMVVGDDS
                     QSIYAFRGATFKNIMEFPTLFPGTTIYKLEENYRSTQPILNLANSIIEQAAEKYTKHL
                     FTRKLDGPLPTLAEAAGENAQSRFIAQKILELREEGVPLNEVAVLFRSSFHSFDLEIE
                     LSRRGLPFIKRGGVKFIETAHVKDLLAHLRVVANPFDTVSWHRVLMLVEGVGPKKAQD
                     LLTTLVKSDKPYDTLRAVTGRSAQGLKNLAQSLETLSGAEDKRTAEQVNHIYEYYLPI
                     LKEQYDDYPKRIRDLDHLHTIAEGYPEVDAFLSDLALEPPDSAVDVDAPDRDEERMVL
                     STIHSAKGLEWQCVFVIWVVDGKFPSLYSFVTDEELEEERRLFYVAVTRAKRHLYLTY
                     PINVFDKGSGMLLSKPTRFLDPVSPDLFDQLALVEEGYQSDWGYGRGEF"
     gene            5276..6151
                     /locus_tag="VFR82_11080"
     CDS             5276..6151
                     /locus_tag="VFR82_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502185.1"
                     /translation="MKRSLLRAFATLSLCLSATAFAAEGLDQSASSAHLWKQITAEAE
                     GLGLPTKFLKMVPSDFVHFEFDDLQAFAAEYHLGEHRMVLNRSLTFNVAGRTLRPLDR
                     LTHAEMETLYHELFHAYMDYLMTLEAQGQPDSVLIFAREQQRCRYGAVLITPVVQRKS
                     ETEERFLSEHESWEALNEAWAVFVGWTVWSQLELRQGPDRSLRKPGKNRDAWLRRLEE
                     ADREGKLRGYYEPEEPAERSVTRKRFLAPQSRLSKEEIERLMAGPLEFSSEFVKQAVR
                     VLTRTQTSMRPSISC"
     gene            6486..6570
                     /locus_tag="VFR82_11085"
     tRNA            6486..6570
                     /locus_tag="VFR82_11085"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6520..6522,aa:Tyr,seq:gta)
     gene            6654..6728
                     /locus_tag="VFR82_11090"
     tRNA            6654..6728
                     /locus_tag="VFR82_11090"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6686..6688,aa:Gly,seq:tcc)
     gene            6830..6905
                     /locus_tag="VFR82_11095"
     tRNA            6830..6905
                     /locus_tag="VFR82_11095"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6863..6865,aa:Thr,seq:ggt)
     gene            6993..>7103
                     /locus_tag="VFR82_11100"
     CDS             6993..>7103
                     /locus_tag="VFR82_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU4502186.1"
                     /translation="MAKAKYERKKPHVNIGTIGHVDHGKTTLTAALTKISA"
BASE COUNT         1497 a         1911 c         2010 g         1685 t
ORIGIN      
        1 cggccaaaaa tgctgccaca tctcgcgaga gacgcggcca tgctcacggc tgaccccgtt
       61 gacatgggct gagaggcgca tcacgagggc cgtcatgttg aaaccgtgtc ctcgtgattc
      121 cggcgtttcc cccagtccca ggaattcatc ccgtgaaagg ttcagctgct cccaataccc
      181 ggcaaaatac cggtccatca agtaatgtgg aaacacgtca tgccccgcgg gcaccggtgt
      241 atgagtggta aatacagtgc tttgtcgcac catgtcgctg gcttcggcat gggagaggcc
      301 tttctgaacg agttccctca ggcgctctaa cgtgagaaat gccgaatggc cttcgttcgc
      361 atgccacacg gaaggcgcaa tatcgagcgc acgcagcagg cggacgccgc cgatacccaa
      421 caggatctct tggcacagcc gcatctcctg atcgcctccg tacaggcgag ccgagagcgc
      481 ccggttctcc gcgctgttct ccggcacatc ggtatcgatc aaatagaggg gaatccgtcc
      541 caccaacact ttccacacga ccgccgtcac attacgacca cccatatcga cggtgaagcg
      601 gcaggcctcg ccagaaggtg tcaacgctgg atgaattgga gagtcttcgc ggttaaacgg
      661 agcataggcg gcttcttgcc aaccctcggc cgtgatccgc tggcggaaat agccttgggg
      721 atacataaag ccgatgccca cgaggggaac gcccagatcg cttgcttctt tgcagtgatc
      781 ccccgccagg atgcccaagc ccccgctgta aatcgggatg gacgtatgca gaccgaattc
      841 cgccgaaaaa taggcaatga cttttttggt ttcgctggca tgctgtatgg tgaaccagct
      901 gcgtccattg gacctatact catcgaacaa tttaagcacg gcggagtatt gtctgagata
      961 cgaaggatcc tcggccagtt tggccaaacg ctccggctgt atgtcggcga gcaatttgac
     1021 gggattgtga tgggtataaa accagagtgt gggatcaatc gtctcgaaga gttggcgtgc
     1081 ttcgacggtc cagctccacc atagattctg agacagctca ggcaatcgtg ccagactggt
     1141 cgggatatgt gtgcgcacag gatcgatgct tgtggtattc acgcagtctc cttgtcaggt
     1201 gaaatcgaaa tagacgagaa gacggtccct gccgaagcga ttacgcgtgg ccggccttat
     1261 gccaagaagt ccactcctcg gagaatgcca cggcggcata ccgttcgcca agcactttcg
     1321 caactcgatt catgagtttt gtgagttgct ggatctcgcc tgcggtaaga tcctggcgga
     1381 accgcggatg caatccccat cgggtttcga tttcgtctcc ggacacatcg gacatgacac
     1441 tgatgagctt gatctcattg cccgtctgac ccctctgcgc tggcgcgccg ggcgacgccg
     1501 cgctgccgct tagctgggac gccgtgtcgg ctaagggcgg tggttctcct ttaaggatcg
     1561 ccgtgatcgc gggaatcatc gcggttgcac acagcctggc ggccgatgtg atttgcggat
     1621 tgtctgccac tcccagcgcg tcggcgatac gcccgccgaa ggcgtcgaac atctcgttct
     1681 tggtcttgct gtcttcagtg atggagaagc ggtagctctc atagagcttg cgactttccg
     1741 cgacaactcc gctgatcgtc atcatgattt cgttctgatc gacgcctgac ccgaaagccg
     1801 gctccagaac ggcgcgcaag cgcgccgtca cagcctcttc cagtccgctc atgaagtccg
     1861 gttggtcttt gaccggaatg tgaaacgccg acagccggtc ggcgaggttg aaggtcacct
     1921 tgaggaactc gagatacaac ttccattcat cttgccgttt gagtttgagc gcctgctcgg
     1981 gggcattttt tttcaagact gtcactgatt cgccggcaac cgccagcatc aggtcggcga
     2041 gcgagcgtcg ctgttcgcta taggacatgt tagccgtgga cataaacgac gtatgctcct
     2101 gagtgttgat agtggggcat tataactgag tgggaatctt ccctccaagg ggttcgagtc
     2161 gactcagaaa cagttgcgca cacgtcaggc gcgtgattgg ggattcctct tggcaaatac
     2221 ccgcgagtcg tccacaatgc ggcgcaaaaa gcgtgaaaag gggcttcgac ttagcgatgg
     2281 aaaatcttcc gctgctcttt gtgcaaacag tcgtactacg cccctacgtg tttgtgtttc
     2341 tcgccgcgtt ccttctcatc ggggggcggc tgctcgggtg gcggagaacg ggagtctttt
     2401 tcctggccag ctggatcacc gcctttgtgt gcgagtattc atccacgcgc attggcgttc
     2461 cgttcgggtt ttattattac accggttcga ccgtcggcca ggagctctac attgcagacg
     2521 taccgttcat ggattcgctc tcgttcacat ttttgctttt ttccagctat tgcctcgccc
     2581 tggtgtttct cttgccttcc gtgaccagag tgggtgggcg gcggctacag ttcgtcctcg
     2641 atccaaagat cagaacgtca tggtcatcgt tgctgttgac ggctacgttt ctcaccgtca
     2701 tcgacgtgat cattgatccc gtcgcgcttc gtggcgaccg ctggtttctt ggacgcatct
     2761 atggctatcc tgacccagga gtgtatttcg gaatcccgct catgaatttc gtgggctggg
     2821 ctgtcgtcgg gcttgtcgga cttggaattt acttttacgt tgatcggagc ctgcccccgc
     2881 tgtccttgca cagcgaccga tctgttgcga ccacgcccct attactgggg tgcacgctgt
     2941 attatggtgt cctgctcttt aatctggtca tcacattctg gatcggtgaa ccgttgctgg
     3001 gtctggttgg aatgatgatc tatctgccca ttacgattgt gtttgttctt cggctcctca
     3061 atcggatccc caccccgcgc ctcgcggttg gaactacatc cacgttggcg ccagacgcgt
     3121 aaccggttgc tctctcgatg tcttcgcgtg ccgcgttgaa acgatctcgt ttataatgtt
     3181 atacagcgca tcacttgtac ttcctccatg agtcgggaca tcacgccata cattctgaag
     3241 cgccaattcg aggacgccgc tcctcgcaat ctatctatcg attatgcggc agccttgaac
     3301 ccccagcagc tggcggccgt cacggcaacc gatgggccgt cgcttgtgat cgccggtgcc
     3361 ggaagcggca agacccgtac gctcgtctat cgtgtggcct atctgattga ctgtggcatt
     3421 gatccttcga acattcttct cctcacgttt acgcgcaagt cggcgcagga gatgttgcaa
     3481 cgcgccggcg agttgattgg aatgcgaagc gagcgggtgt gtggaggaac atttcactcc
     3541 gtcgccaata tgctgctgcg gcgctatggc aggggcgtcg gcttggagcc gggattcacc
     3601 attttagatc gaagcgatgc cgaagacctg atcgcgctcg tgcggtcgca actcgggctg
     3661 aatgaaaaag acaagcgatt tccgcggaag agcaccatcg cggaaatctt cagcaaatca
     3721 gagaacacgc tgcgcagctt ggatgacatt cttatggagg aatttagcca ctttgccgat
     3781 catattgatg cgcttggcca actccagaca ggctaccgag cagcgaaacg ccaacggcaa
     3841 ttagtggact atgacgatct cctcgtgctg ctccgccaac tgctcatgca ggatccggcg
     3901 gcgcggcaat cgatttcgcg gttgtatcgg tacattctgg ttgacgagta tcaagacacc
     3961 aatagactgc aggcggacgt cattaggaat ctggcggtat ctcatcagaa tgtgatggtg
     4021 gtcggagacg atagtcagtc gatttatgcg ttcagagggg cgacgtttaa gaacatcatg
     4081 gagttcccga ccctgtttcc cggcacgacg atctataaac tggaggaaaa ctaccgaagt
     4141 acgcagccga tattgaacct ggcaaattca atcatcgagc aggcagccga gaaatacacc
     4201 aaacatctct ttacgcgaaa gctcgatgga ccgctgccaa cgctggctga agccgccggg
     4261 gagaatgcgc agtctcgatt catcgctcag aaaatcctcg aattgcgaga ggaaggcgtg
     4321 cctctaaacg aggtggccgt gctgttccga tcgagctttc attcgttcga tctcgaaatc
     4381 gagttgtcgc ggcgggggtt gccgtttata aaacgcgggg gcgtcaagtt catcgagacg
     4441 gcccacgtca aagatttatt ggcgcacttg cgtgtcgtgg cgaatccttt cgacacggtg
     4501 agttggcatc gggtcttgat gctggttgaa ggggtaggcc ccaaaaaggc tcaagatctg
     4561 ttgacaacct tggtgaagtc ggacaagccg tacgacacgt tgcgcgccgt cactgggcgt
     4621 tcggcgcagg gtctgaaaaa tttggcccag agtctcgaaa ctctttccgg agcggaggac
     4681 aaacggaccg ctgagcaggt caatcatatt tatgagtact atctgccgat tctcaaagag
     4741 cagtacgacg attatccgaa gcggattcgg gacctcgatc atctgcatac cattgccgaa
     4801 gggtatcctg aagtcgatgc cttcttatcc gatcttgcgc ttgaacctcc agacagcgcg
     4861 gtggacgtag atgcaccgga tcgtgatgag gaacggatgg ttttgtcgac tattcactca
     4921 gccaaaggat tggaatggca atgtgtcttt gtcatttggg tggttgatgg aaaatttccc
     4981 tcgctctatt cgttcgttac cgacgaagag cttgaggaag aacggcgcct gttttatgtg
     5041 gccgtcaccc gcgcgaagcg gcatttgtac ctcacctatc ccatcaatgt gttcgataag
     5101 ggcagcggca tgttgctctc gaagccgacc cggttcctgg atccggtttc tcccgacctc
     5161 tttgatcagc ttgccttggt tgaagagggg tatcagagcg actggggtta tgggcggggt
     5221 gagttttagg cttgtttggc ctgtggagct gttcctggtt gcacctctcc agtcaatgaa
     5281 acgttccctc ttgcgcgcgt ttgccacgct gagtctttgt ctgtcggcaa cagcattcgc
     5341 cgcagaaggt ctcgatcagt cggcctcctc ggcgcatctc tggaagcaaa tcacagcaga
     5401 agctgaaggg ttgggactgc ccactaagtt tctcaaaatg gtgccgtctg acttcgttca
     5461 ctttgagttt gacgatctgc aggccttcgc ggcggaatat catctcggcg agcatcgcat
     5521 ggtgttgaat cgatccctca cctttaatgt ggcaggtcga acactgcgac ctctggatcg
     5581 actgacacat gcagagatgg aaacgctcta tcatgaactg tttcacgcct atatggacta
     5641 tctgatgact ttggaggcgc aagggcagcc cgactccgtg ctcatatttg ctcgcgagca
     5701 gcagcgctgt cgatacggcg ccgtcttgat cacgccggtc gtccagcgaa aatcggaaac
     5761 cgaggagcga tttctcagcg agcatgaatc ctgggaggcc ttgaatgaag catgggctgt
     5821 ttttgtcggg tggaccgtct ggagtcagct ggagctccga caaggaccgg accgctcact
     5881 tcgaaagccc ggcaaaaacc gcgacgcatg gcttcggcgt ttggaggagg cggacagaga
     5941 gggaaagtta cgcggatatt atgaaccaga ggagcctgcc gaacgtagtg tgacgagaaa
     6001 gcgttttttg gctcctcagt cgcggctctc gaaagaagaa attgaacggc tcatggcggg
     6061 acccttggag ttttcatcag agtttgtcaa gcaagcggtc cgtgttttga cacggacgca
     6121 aaccagcatg cgtccatcca tatcctgtta attaagcgta caactgcttg aatagcatgc
     6181 cttttatcga ttcacgccct aggcccagct ctccatacac ggaagtcatg cgacaggaaa
     6241 tgacttggct ccttgtacag agcccgctta aaattctttc ggggtcgtgg gtacttgact
     6301 taaagttact tcatcaatag aatcagccgt ttcctgaaca aggctgtatc cgctgctcat
     6361 cacttcctct ttaattttga ggacgggagg tgctgtgcgt ggaccagcct actcttcttt
     6421 tcccatcaat atactgatgc gaaacgagga caacttttcg attggaagta ttgcgcagga
     6481 gatttgggca ggtacccaag tggccaaagg gggcagactg taaatctgtt gccatcggct
     6541 tccaaggttc gaatccttgc ctgcccacca aagcaggcag ttgctgacgg cagtgcggag
     6601 tgtttgaaca agcgccgctc cggcgttcac gctcacatcg tgttgcaatc aaggcgggcg
     6661 tagctcagcg gtagagtccc agccttccaa gctggctgtc gtgggttcaa ctcccaccgc
     6721 ccgctccaag aaagacgtga gatgccatta aggcgagtac atcgagcatg gacgatcgaa
     6781 actgaccacc aacgggaccc cttcgcgaat ggcgtatttc tctggcatcg cccacgtagc
     6841 tcagttggta gagcacgtcc ttggtaagga cgaggtcacg cgttcgatcc gcgtcgtggg
     6901 ctccacgtcc ggctgcgctt cgggcatgag cctttcgtga cagggcgctc gacgggagcg
     6961 atctctttgg tttttagcaa aggagttgag gtatggcgaa ggcgaaatat gagcgcaaga
     7021 agccgcatgt gaatatcggg acgatcgggc acgtggacca tgggaagacc acgttgacgg
     7081 cggccttgac gaagatcagc gcg
//
LOCUS       DASTQY010000338        20698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1320878, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000338 DASTQY010000000
VERSION     DASTQY010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 20698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20698
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320878"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VFR82_11105"
     CDS             complement(<1..226)
                     /locus_tag="VFR82_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502187.1"
                     /translation="MALDAADSIQAKNSPEKMMAHQLAAVHRVVMDHLAFVPSQYDVA
                     AQAKRLNAVARCMSVYQQGLLTMRKLRQGGH"
     gene            complement(1107..3275)
                     /locus_tag="VFR82_11110"
     CDS             complement(1107..3275)
                     /locus_tag="VFR82_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEU4502188.1"
                     /translation="MSEVIRWNDQFYILASSSLAANRVEVLKHGETFAVFDRYGDIHQ
                     VMPGPQGLYHEGTRFLSQFELTLGTHRPLLLSMTVKEDNALLTVDLTNPDLTSDGQDL
                     VPRGTLHLCRTRFLWNGSCHERIRIHNYGNSEFPLAIKIAVDADYADLFEARGQQRER
                     RGQRLQTLSSREGLVLGYEGLDHVVRRTHVRCWPTPDHVAVGGLILESFVAPRSDVSW
                     ELAIACEIEPQHSQVCLSYDRARTESDCALKAARQQDCHIYTSNEHFNDWLNRSIADL
                     DMLISETPQGPYPYAGVPWFSTPFGRDGLITALELLWVNPRVARGVLGFLASTQATKD
                     IPERDAEVGKILHETRRGEMAALGEIPFGSYYGSVDATPLFVMLADAYYVRSGDLEYI
                     TTIWPNIERALDWIDRYGDPTGTGFVTYDRQSSNGLVHQGWKDSQDAVFHADGTAARG
                     PIALCEVQAYVYRAKKAAAGLARLMGDTDRAEMLILEADSLRERFERAFWCENIGTYA
                     LALDGDGHPCRVRSSNAGHCLLAGIAEPKRGLRTARTLLAEESFSGWGIRTIPTSEAR
                     YNPMSYHNGSVWPHDNALIAAGMAHYGDKQGTVKILTGLFDASLFLELRRLPELFCGF
                     PRRPGESPTRYPNACAPQAWASAAGFLLLQSCLGLQIDAPRRRLSFVRPVLPPFLERV
                     EIRNITIGDARVDLTLDRHPEDVGLAVTRREGEVEIMVIK"
     gene            complement(3272..4579)
                     /locus_tag="VFR82_11115"
     CDS             complement(3272..4579)
                     /locus_tag="VFR82_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4502189.1"
                     /translation="MQGRSSPAACHLPAVSEAELLATESRPLLLTRNFGLVWSGQLIS
                     QIGDGISRLALLWFVYAITGSALKTSMIGLLQTIPPILLGPVIGVYVDRLPKKYLLII
                     SDLLRAVLIGFIPCMVAVESFTVSMLYVLVLLHAIATAVFGPALTAAVPAIVPKTQFT
                     AANALLQSTTSLGIILGPALSGLGIAAYGSQEVLCLNAITYLVSALCLSLTCFTGSRT
                     TVGSQAARSTLMQDLIEGFRYSVVHRPAILLLTGTAALYTFGTAALTSLFPVFARKLL
                     DLGPVEIGYLWSALGGGLLLTSIGLLWATDWTVNDRSKLIFGTSLLSGVAVCALVWTP
                     HAYQAGILMGLIGGGMGAFTPIAWGIIQELTPGHLVGRVLGLYGTGAMTSAIAGISSF
                     GWITERYGPGPGLLGIGIVMLATALVALRMSHVSSPALRESRL"
     gene            complement(4579..5328)
                     /locus_tag="VFR82_11120"
     CDS             complement(4579..5328)
                     /locus_tag="VFR82_11120"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU4502190.1"
                     /translation="MMPSCRVLLRATMLVICLFPISADVALPEQAPALDNQPAAPKPS
                     SETVQPPDASTAKPEPPKLSTEPKPLPQSAPPAILEAPSERETHEKGKEKELRKKPMG
                     SLILTVKLALLADARLFHYEIEVGEDGQSITLGGRISSEEEKAAATEIARSVSDGKTV
                     VNKLEVDKGLLHALGKKQDEIISTLVKDRFAKSATLKAANFEVKTEEGVVSLSGAVRF
                     QVLALEAAEAARLVPGVRAVKTDRVRLGGES"
     gene            complement(5412..6740)
                     /locus_tag="VFR82_11125"
     CDS             complement(5412..6740)
                     /locus_tag="VFR82_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4502191.1"
                     /translation="MSDSRALNEKAQTGSRGWELLKTRDFGLLFAGQTISQIGDSLNK
                     VALLWFVYEMTGSALKMTVIGLLQTLPPLLLGPLIGVYLDRFRKKPVMITVDLLRTVM
                     ILLIPLLYLVEALTMDRLYLLVFANAIVSTVFGPALASAVPLIASKDRLVAANALMQS
                     TANIGLLVGPAVSGIGIALIGAQNVLYVDAATFFVSALCLMPIRTQETLQPNLSGKGW
                     TEGVASDLLAGFRFVFVEQKTVLLLMLTATLYSVGFSAFVFLLPVFAKDVLAVGPIQL
                     GWLWSALGVGMLIASFGLASIGQGDFPRRLHLISFALAVGGLAVGTLGFLEAPLAAGA
                     VIAVIGVSTAMFTPLVWTMLQELTPAPLLGRVFTSFATGGMAASMAGMAGFGWATDML
                     GPGVSLGGISCILLITAAAVGTLGWYRSQMQGLPAAEPGAFAPRTIVAAS"
     gene            complement(6733..7887)
                     /locus_tag="VFR82_11130"
     CDS             complement(6733..7887)
                     /locus_tag="VFR82_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4502192.1"
                     /translation="MRIAQVAPLWESVPPKLYGGTERIVSYITEELVQMGHDVTLFAS
                     GDSETAGRLQAVCPQALRLNTGIFNRDAPMIMLQERSLGARGDFDVIHSHLDFLGFPL
                     ARRNPQPVVTTLHGRLDLPELQPVFQEYAEMPLVSISDAQRRPLPWANWHATVHHGLP
                     RHLYTYHSQPQGYLAFLGRISPEKRPDHAIEVAKRTGLPLRIAAKVDPADIQYYRSEI
                     EPLLDHPLIEFIGEISDAEKDDFVGNAQALVCPYDWPEPFGLVLIEALACGTPVLAYR
                     RGSIPEVIDDGITGFVCENLSEMVAAVERLGEIDRQRCRASFEERFTADRMARDYVAL
                     YERIIEDRGAVTTPKIHALGSDLLFEGAFNGTSNGHSQDGPLHIEGGPHV"
     gene            9158..10219
                     /locus_tag="VFR82_11135"
     CDS             9158..10219
                     /locus_tag="VFR82_11135"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502193.1"
                     /translation="MKALLPVALAIKTTISVLLITFILFEAVLRLYHFVNPLFVFPDN
                     SDNRFRGKPYGDDYDFTLNSKGFKDIEFRSEKVNGTRRLVGIGDSFVFGTVPYKYNFL
                     TVFEEEWNRAQTASPIEVFNMGIPSTSPRQYLSVLIKDALPLQPDAALVFIYIGNDFI
                     EISKKPLLQHSFVIAAGKFVFDLIRHLEDGVTPHSRTSQYKDDAATLSVDKFLEIETG
                     RSDIYALDSKLLPERLPRVMYFVDEINRVCKEKDIALYIVLIPDEVQIDPKLQQDVLA
                     ALQLPTNMFDFDLPNKRLAQELQGRGIKHLDLLPAFRDASREERRLYIPRNTHWNIEG
                     NRLAAALLIEFLRHELSTS"
     gene            complement(10631..10918)
                     /locus_tag="VFR82_11140"
     CDS             complement(10631..10918)
                     /locus_tag="VFR82_11140"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEU4502194.1"
                     /translation="MRGLWKLLIGLSAITLWACAEEHGKGYWLRHATVTLAQAAQIAE
                     KNGPGRAVAAQLGQSGTRVFYDIEIIDAFNKSRRLRVDAETGKIIQGLGLP"
     gene            11511..11924
                     /locus_tag="VFR82_11145"
     CDS             11511..11924
                     /locus_tag="VFR82_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502195.1"
                     /translation="MADPKSILLIDGSARDRRYYGSVVQGALPDCLILEAENGEDGLK
                     LFQSKSIDCVILELALPDIHGTDLLKQLAVPACLPRRPIIVLTRLFYPTILTIARENG
                     ATATFVKKVTSEQALQDAVLAALSTIPASSSAEKG"
     gene            complement(12683..14218)
                     /locus_tag="VFR82_11150"
     CDS             complement(12683..14218)
                     /locus_tag="VFR82_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEU4502196.1"
                     /translation="MTFGLLVPLSVAAQSNGNSRVALDFNDVELSVFVRFISELTGKN
                     FVLDDAVKKAGGKITVFSPTKVTPDQAYSLFVSALEVSRLAVVPRGPVTQIVQMGELP
                     PERGVFVYKLKHANATDLAAVLTNLVARSQTVSQTTPGIRPPIRPLTEFEAPVQVFAD
                     KATNSIIISSTKSAYARLQSVIRDLDGRRKQVFVEAVILEVQVDRLRQIGTDPVQVLA
                     AGKSGQFQGIGSINRAPEDIATIAQTISGIAAGTSTGGAVSVLNTINIRAFLQLLLSL
                     TDTNVLSTPQVLAADNQKAKIVVGENRPFPTGQAQGITGGTLVTIERKDVGVTLELTP
                     QVLENDLIRLEIKQEITAIAEAVAQTIGTGVATVPVGPTTTKRSMETTTIAKDGQTLV
                     VGGLVRDNLTLSERKVPLLGDIPLLGWLFRFQSRQSEKLNLLVFLTPSLVRDEVDMVD
                     LNARKSAELNSLQRENRVEEPTRLKQDVIEKLQRPTTPPPPTHPTQPTEPLVLPPPVS
                     KPE"
     gene            14695..18372
                     /locus_tag="VFR82_11155"
     CDS             14695..18372
                     /locus_tag="VFR82_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502197.1"
                     /translation="MRIDKRSSQRTDKKAPSRVHKRGVVGYSKKTALLEEAITHMNTG
                     KYGRSSAALKELLALDPQNTEARRLFATLHLRLGSLVTARQAFESLANEAIGRQDYWL
                     AESLLGEYLAAGPRCVPFLEQLAYVYQEKGDELAAVGELGKAIEILRDDPDPDNPQKA
                     AQLYSKIRELAPASSVAMQLAPLFDVQTGEFLVCPSAQSPAHADASSSLADASAAAHV
                     DHPLPEVMPWEVTEETSPVPDSEASLAASYAASEEAALSTSCVIEDVGSLDQTVSEPV
                     QSTPVDEPPLSFTLRTEETLLEPSADASDTTTVPSESVLPQAWGGMPSHMPWEQMADA
                     SIRIIEPESPPPDPGLLESILSSLRTEDTSVASEGPVAEATASQVEAEEPMASAPAPV
                     TDGDIAFVSLSSPMPWEQVSDAAMQIPAEEPTPLPLVEDRPFHIATAEPEPSALSTAG
                     DSCPFQPATDQGASPSSDSNPPADSPPLPSADSAKPASFSWNSVFDKAWEFTSGTMAP
                     SPPVPSRIDPEPRSQGQPGEAAPLPVTADGPVSEQSIAVECSPPIETPLSPLEPAVSA
                     SSSAVVEEGAIHEYVVESTSPPAVPSEHWNTGEVAIQVHRPSKKHKRSRHGPEEAEPK
                     ASPAAAPTTESLSDELSQTKREWISASDEAAPLIEEPVSVSPDTRPDWMQATDAITFD
                     RPAPPPAATWSDAPAVTASPVEVEVAPSAAASAVDMLFARSGPIDDARPHVSLWSKPA
                     PRLIARLHRIRISIWSFIWSCFSTTRSLTFLVLMTAVAVVVVVAAGLAAIGLTWMAME
                     DAPSPLYQNLTISPPRTITDAKKNGYLLLLGFDAPAGLDPLQVGYERKPGLQDKTAAQ
                     LCSAGDEAKDTGSAGASGQVVRGWVRSGDFLSQVKGQSGSIRSFVSRESVTLARYQQW
                     LAMPFDDWGFGQSLSPNCSRVLLAHRLFVLEGFNQDLSSGIDRLETDMQSWRAALGQS
                     KTLAMKMLAVTAVQDDASVASGLLSRAELDGAALARLTKMVRPLDQAELSVRWPMHSQ
                     FVWATKSVSAELKDDQTRDRPWHATMASAMRLPIQRRANAYAEYYEAANKAVAGGRYT
                     NLPKLSSFVRTPPSGVMDYLVNPVEHIVGVEPLPSWDPYVMRIVEADAELRLAGLQAW
                     IRRGPQEGDVLTRLAKAGQASYDPFTGLPMLVNQRKGLIYSVGRDGKDQEGDNTRDLA
                     VPIPIVSSSDSRRAPLSPPVR"
     gene            18504..19973
                     /locus_tag="VFR82_11160"
     CDS             18504..19973
                     /locus_tag="VFR82_11160"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU4502198.1"
                     /translation="MKPHSTSRLRQGAHSTGPIPTQIVSNEEFTPWPRTPEQALVEEA
                     VVRTSRKAAVRLGMTRRHFLKTSGGMAAALLALNSVFGRFFEVFEAELFEPAAFAEQQ
                     GASYFVFDVQTHYVSTGYDPSDSEVRRRGAVSKEALLGLRRRARDSGLNPRLTADRGT
                     IDDLSWQNFVKEVFLDSETSVGLISTPPGPYPQEAVVPPKEMAHIRDELNRLAGSQRM
                     LAHGLATPQLGATDLEFMAQQAEQLKIDAWKCYTGSCPRGFDHGWWMDDEKIAYPMLE
                     QARRLHVPRVCVHKGLPLGPVPDYNHPRDLIKAAKDFPDIDFLVYHSGMKSAQSIDEV
                     FGKTGEIPWTTEFCRMKQAHPGLKNIYMELGSTFGQLVTSHPLVCAHLIGQILQAFGD
                     DHVLWGTDSIWYGTPQWQIEAFRRFQMPDHLIEQHHYPSLTREVKAKVFGLNAARLFA
                     VDPAATRQGIPKDYVGRLRLSYLEEGPEPSHRLYGWVAT"
     gene            20001..20684
                     /locus_tag="VFR82_11165"
     CDS             20001..20684
                     /locus_tag="VFR82_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEU4502199.1"
                     /translation="MKHEILYPGAFPMVRVELAAGETIKAESGAMVASSPTIDIESKM
                     EGGFLGALSRKLLTGEKFFFQTLRASRGAGEVLLAPTVPGEIVVMELDGLNEYMVQKD
                     GFLAGSESIKIESQMQSLSRGLLGGEGFFILKVSGKGSLILNSFGAIHKIELKPDQEY
                     IVDNSHLVAWSGTTSYHIERAASGWVASFTSGEGFVCRFRGPGVVYIQSRNPGSFGAW
                     VRQFIPVSE"
BASE COUNT         4585 a         5888 c         5886 g         4339 t
ORIGIN      
        1 gatgtccgcc ctgccgcagt tttctcattg taagtaaccc ctgctggtaa actgacatgc
       61 atctagccac cgcattaagg cgtttcgcct gggccgctac gtcatactga gacggcacaa
      121 acgccaggtg atccatgact actcgatgta ctgcagcaag ttggtgcgcc atcatcttct
      181 ctgggctgtt ttttgcttga atcgagtcag cggcatccaa ggccatggcc gctacgcccg
      241 ggccctgccg aaccagaagc tgtgtccttt caaaggatgc ttcgacagaa gcgaggtcag
      301 gaactgccag cgtgtcgtgg agaaacttct gtgcttccgt ggggagggct tctccattag
      361 ctagcccttt tctttagaag gtgcttcccc tcttttaaga attgtgcctg caatagactt
      421 ggcccatccg gcacggttcc gtggctcttc tgcattccgc ccttctgact caagatcaag
      481 ggcgtcccgg gactctcttc catattcgag catcttattc atcgacttga ggtgcagtcg
      541 ttgcctggca aggacttttc caaacaagaa aaacaacgcc acttgtcatc cggccagaga
      601 tattcaccaa ggacaggagg ttttttgcat tcgtcgcaag tagccggagg aggaaaccac
      661 agggatgcag aacaagagat gtgctgtact gagggacatt gtgacagatc gccgaagcgg
      721 gcgagatgta ccgtcgtatc gccaacacga atcggcgtta caaggacgcc gccttcctag
      781 cacagcttgt cgtggaaggt gagaaacctg accaatggcg aacggcctgc ggacaaccag
      841 tcgtggatcg gcaatgccat caagagtttc ccgcgctggc cccttctcgt ctcccgataa
      901 gatcaacgcc attccccgtt tgtcgaggta gcaggacatg aggtaggtgc gtcgacctcg
      961 aaaataaacg ttctggggaa accaaacaca ataacgagca cttaccaaga acggcctatt
     1021 tatcctttta atgcgaggac ctgagttcga gtcccaggcg gctaaccacc aattccatca
     1081 gagttcgtct gattctgttg atggaactac ttgattacca tgatctctac ttcaccttcg
     1141 cggcgcgtca ccgccagacc gacatcctca ggatggcggt cgagagtcag atcgacacgg
     1201 gcgtctccga tcgtgatgtt gcggatttcg actcgttcta gaaacggtgg cagaaccgga
     1261 cgtacaaagc tcaggcgacg gcgtggggcg tcgatctgca gaccgaggca cgattgcagc
     1321 aagagaaacc ccgcggcgga ggcccaagct tgcggagcgc aggcgttggg gtagcgggtc
     1381 gggctctcgc cgggtcgcct cgggaatccg cagaacaatt cgggcagccg gcgaagttcg
     1441 aggaacaagc tcgcgtcgaa cagtcctgtg agaatcttta ccgtgccctg cttgtcgcca
     1501 tagtgggcca taccggctgc aatcaatgca ttgtcgtgtg gccagaccga gccgttgtga
     1561 tacgacatcg gattatagcg cgcctccgat gttgggatcg tccggatgcc ccagccgctg
     1621 aaggactctt cggccaagag cgtccgagcc gtcctcagac cgcgcttggg ctctgcaatg
     1681 ccggccagca gacaatgacc ggcgttcgaa gagcgaacgc gacaaggatg accgtcgccg
     1741 tccaatgcca gtgcatacgt gccgatgttt tcgcaccaaa aagctcgttc gaaccgctcg
     1801 cgcagtgagt cggcctctag aatcagcatc tcagcccggt cagtgtcgcc catcagacgc
     1861 gcgaggccgg cagccgcctt tttggcgcga tacacgtacg cctgaacctc gcacagtgcg
     1921 atcggtccgc gtgcggctgt gccatccgca tggaacacgg catcctgaga atccttccag
     1981 ccctggtgca cgagtccatt ggaggactgc cggtcgtaag tcacgaagcc ggttcctgtt
     2041 ggatcgccgt agcgatcgat ccagtccagc gcgcgctcaa tgttgggcca gattgtcgtg
     2101 atgtactcaa gatcgccgct ccgcacataa taggcgtcgg cgagcatgac gaagagaggc
     2161 gtggcatcca cgctgccata gtagcttccg aaggggatct cccccagcgc ggccatttct
     2221 ccacggcggg tttcgtggag aatcttgccg acttcggcgt cccgttcggg aatatctttg
     2281 gtcgcctggg tggaggccag aaacccgagc acgcctcgag ccacacgcgg attcacccag
     2341 agcagctcca gcgccgtgat gagtccgtcg cgtccgaagg gggtgctaaa ccagggaacc
     2401 cctgcatacg ggtacggtcc ctgcggagtc tcagaaatta gcatgtcgag atcggcgatg
     2461 gaccgattca gccagtcgtt aaaatgttca ttagacgtgt agatgtggca atcctgctgc
     2521 ctagcggctt tcagcgcgca atccgattca gtgcgggcgc gatcatagga aagacacacc
     2581 tgtgaatgct ggggctcgat ttcgcaggca atggccagtt cccacgagac atcactgcgt
     2641 ggcgccacga atgattccag tatcagtcct ccgaccgcca catggtccgg ggtcggccag
     2701 caacggacat gagtccggcg aacgacgtga tcgagccctt cgtagcccag cacgagtcct
     2761 tcgcgggagg agagcgtctg gagccgttgg ccgcggcgct ctcgttgctg gcccctcgcc
     2821 tcgaacaggt cggcatagtc ggcgtccaca gcaatcttga tcgcgagagg gaactccgaa
     2881 ttgccatagt tatgaatgcg gatgcgctcg tggcaggacc cattccagag aaacctggtg
     2941 cggcagagat ggagggtgcc tcgcggtacg aggtcctgtc catcggaggt caggtccggg
     3001 ttggtgagat cgaccgtcag gagcgcgtta tcctccttca ctgtcatact gagcagaagg
     3061 ggcctgtgtg tgccgagcgt gagctcaaac tgcgaaagaa aacgcgtccc ctcatgatag
     3121 aggccttgtg ggcccggcat gacctggtgg atgtcaccgt agcgatcgaa gaccgcaaac
     3181 gtctctccgt gtttcaaaac ttccacgcgg ttcgccgcca gcgacgaaga cgcgaggatg
     3241 tagaactgat cgttccagcg gatgacctcg ctcataagcg actctccctt aatgccgggg
     3301 aactgacgtg gctcatcctc agggccacga gcgcagtggc caacatcacg atcccgatcc
     3361 cgagcaggcc gggtccggga ccatagcgct cggtaatcca accgaaggaa gagatgccgg
     3421 cgattgcgga ggtcatggcg ccggtcccat acagccccag taccctgccg accaggtgcc
     3481 cgggggtgag ttcctggatg atcccccacg cgatcggcgt gaacgctccc atgccgccac
     3541 cgatcaaacc catcaggata cctgcctgat aagcgtgggg cgtccagacc aaggcacaga
     3601 cagctacgcc gctgagcagg ctggtaccga agatgagttt gctgcggtcg ttgacggtcc
     3661 agtcagttgc ccacaggagc ccaatcgatg tgagcaataa tccgcctcct aatgccgacc
     3721 acaggtagcc gatctctacc ggacccagat cgagcaactt tctggcgaag actggaaaga
     3781 gcgatgtcag cgcggcagtg ccgaacgtat agagtgccgc ggtgccggtc aaaagcagga
     3841 tggcgggtcg gtgcaccaca ctgtagcgaa atccttcgat cagatcctgc atcagcgtcg
     3901 agcgggcagc ttgtgagcca acggttgtgc gggaaccggt gaagcacgtc agcgacaggc
     3961 agagggctga taccagatag gtgatggcat tgaggcacag cacttcctgg gaaccgtagg
     4021 cggcgatgcc caatccgctc agcgccggac cgaggataat acccagactc gtcgtgcttt
     4081 ggaggagcgc attggccgcg gtaaactggg ttttcggaac gatggccggt accgccgcgg
     4141 tcagcgcagg tccgaacacc gccgtggcga tggcgtggag caggacgagg acgtacagca
     4201 tggacacggt aaacgactcc accgcaacca tgcacgggat aaagcctatc agcacggccc
     4261 gaagaagatc actgataatc agcagatact tcttgggcaa gcggtcaacg tagactccga
     4321 tgaccgggcc gaggaggatc ggcggaatcg tttgcaggag gccgatcatc gatgtcttca
     4381 gcgccgagcc ggtgatggcg tacacgaacc agaggagcgc cagccgggag atgccgtcgc
     4441 cgatctgcga aatgagctga ccggaccata ccagtccgaa attgcgggtg agcaataggg
     4501 ggcgagactc ggtggcgagg agctcggctt ctgagaccgc gggaagatga cacgcggcgg
     4561 ggctggaccg gccttgcatc agctctcgcc cccaaggcga accctgtcgg tcttgaccgc
     4621 tctaacaccc ggcaccaagc gggccgcttc cgctgcttca agggccagga cctggaaacg
     4681 cacagcaccg ctgagcgaaa cgacgccctc ttcggttttc acctcgaagt ttgcagcctt
     4741 caacgtggcg ctcttggcaa accgatcctt cacgagagta ctaatgattt cgtcctgctt
     4801 cttccccagc gcatgcagca gacccttgtc cacctccagc ttgttgacga ctgtttttcc
     4861 gtcagacacc gatcgcgcga tctcggttgc agccgccttt tcctcttcac ttgagatgcg
     4921 tcctcccagc gtgatgcttt gcccgtcctc cccaacttcg atttcataat ggaataggcg
     4981 cgcgtcggcg aggagtgcta gcttgaccgt caatatcaat gaacccatcg gtttcttgcg
     5041 gagctctttc tcttttccct tctcgtgcgt ctcgcgttcg gacggcgcct ccaatatcgc
     5101 gggcggcgcg ctttggggca agggtttggg ctcagtcgac agcttcggcg gttccggctt
     5161 tgcggtcgat gcgtccggtg gctgcaccgt ttcactagaa ggtttcggcg ctgccggctg
     5221 attgtcgaga gctggagcct gctccgggag cgccacatcg gctgaaattg ggaacaggca
     5281 aatgaccaac atcgtggcac ggagcaggac tcgacaagag ggcatcatgc ggagcccctc
     5341 cccatggctt cggcaaccgt gacggatgac taccatcggc atctaaaatg tcaaggcagc
     5401 aacaacgtgg atcacgaagc ggcaacgatc gtccgcggag cgaacgctcc tggttcggcc
     5461 gcagggagac cctgcatctg cgacctgtac cagccaagcg taccgactgc ggccgccgtg
     5521 atcaacaaaa tgcagctaat ccctccgaga ctgactcccg gtcccagcat gtcggtggcc
     5581 catccgaacc ctgccattcc cgccattgag gcggccattc cgcccgtcgc gaagctcgtg
     5641 aagacacggc cgagcagggg agccggtgtc aactcctgca gcatggtcca gaccaacggc
     5701 gtgaacatcg cagtgctgac tccgattacg gcgatcacgg caccagctgc taaaggagcc
     5761 tccagaaatc cgagcgtgcc gacggcgagc ccgcccaccg ccagcgcgaa ggagatgagg
     5821 tgcagtcgtc gaggaaagtc tccctggccg atcgacgcaa ggccgaagga ggcgataagc
     5881 atgcccacac cgagtgcgga ccagagccac cccagttgga tcgggccaac ggcgagcacg
     5941 tccttcgcaa acaccggcag taagaatacg aacgcgctga agccgacgct gtacagcgtg
     6001 gcagtcagca tcaacagcag gacggtcttc tgttccacga acacaaaccg gaagcctgcg
     6061 agtagatccg aggcaacgcc ctcggtccag cctttgccgc tcaagttagg ctgaagcgtt
     6121 tcctgggttc gtatgggcat caggcagagg gctgaaacga aaaaggtcgc ggcgtccaca
     6181 tagagcacgt tttgggctcc gatcagcgcg atgccgatcc cgctcaccgc gggaccgaca
     6241 agcaagccga tgttagcggt ggattgcatc aaggcattgg cagcgacgag ccgatccttg
     6301 gaggcgatca gcggaaccgc cgaggccagg gccggtccga agacagtgga cacaatggca
     6361 ttggcaaaca ccagcaggta caggcgatcc atagtgagag cttccaccaa atagagcaat
     6421 gggatgagca gtatcatcac agtccgtaac aggtcgacgg tgatcatgac cggtttcttg
     6481 cggaaccggt cgagatagac accgatcagc ggccccagga gcagcggagg aagggtctgc
     6541 agtagtccga tcaccgtcat cttgagggcc gacccagtca tttcatagac aaaccagagc
     6601 agcgccacct tgttcaggct gtcgccgatc tgtgagatcg tttgtccggc gaataggaga
     6661 ccgaagtctc gcgtcttcag caactcccaa cctcgggaac cggtctgcgc tttttcattt
     6721 agggcccgac tgtcagacat gtggccctcc ctcaatgtgc aacggcccgt cctgcgagtg
     6781 tccgttggag gttccgttga aggctccttc gaagagcaag tcggatccga gagcgtggat
     6841 tttcggagtt gtcacggcac ctcgatcttc gatgatccgt tcatacaggg caacgtagtc
     6901 acgggccatc cggtccgcgg taaacctctc ttcgaaggag gcacggcagc gctggcggtc
     6961 gatttcccca agccgttcca ccgccgcgac catttcagaa agattctcac acacaaaacc
     7021 ggtgatgccg tcgtctatga cttccggaat ggacccgcgc cggtaggcca gcacgggtgt
     7081 cccacaggcc agggcttcga tgaggaccag accaaacggt tcgggccaat cgtacgggca
     7141 gactaatgcc tgagcattgc cgacgaagtc gtctttttct gcgtccgaaa tttctccgat
     7201 aaattcgatg agcggatgat cgagcagggg ctcgatctcg gaccggtaat attggatatc
     7261 agccgggtcc accttggccg caatgcgtag tggaaggccg gttcgcttgg ccacctcaat
     7321 tgcgtggtca ggccgtttct caggggagat tctccccagg aatgccagat atccctgcgg
     7381 ctgtgaatga taggtataga gatgtcgggg aagcccgtgg tggacggttg catgccagtt
     7441 tgcccagggc agcggccggc gctgagcgtc ggagatcgac accaacggca tctcagcata
     7501 ttcctgaaag acgggctgca gctccggcag atccaatcgt ccgtgaaggg tggttacaac
     7561 cggctgggga tttctccgtg ctaaaggaaa cccgagaaag tccaggtgcg agtggatcac
     7621 gtcgaaatcg ccccttgctc cgagtgagcg ttcctgcagc atgatcatcg gggcgtcacg
     7681 attgaagatt cccgtattga gccgcaacgc ctgaggacac accgcttgaa ggcgcccggc
     7741 ggtctcagaa tccccgctgg cgaagagggt gacgtcatgt cccatctgca ccagttcttc
     7801 ggtgatgtac gagacgatcc gttcagtgcc cccgtacagc ttagggggca cactttccca
     7861 taggggagcc acttgggcga ttctcatgag ttcttccttt cagaaagatg ttgaatgtaa
     7921 acgagaaaga atggctgacc aacagacctt gattgcggta ggaccgaaag gagcctgacg
     7981 cgatccttgt acgcgcaagg caaccgagaa gggccaaacc cataccgtat gtcgtatcac
     8041 tgcacggagc tacccggcac cctcacagcc cggttactag aaacataccc gaggtccggg
     8101 cctatgcacg catcgttctc ggcgaagtga ccgttcaagt gaggagacga agagggtatc
     8161 aatagctaga ttagttgcgg ggggggacgc ccggactcgc aatgagagcg gatttcgact
     8221 ggaacgagca cctaagagaa tggcagtacg cggaaggcgt agacggctga agccagctac
     8281 cacctccctt gtcgaacact atgggtgtat ccgaggccat acaggtcttt taggccagac
     8341 tgaataccta aattaatgaa cctagcttag tagcactaac gccgtgccgg tcacggcctc
     8401 gctctctata gatgtatgag agaccataca atcctgcagc gataagcttg ctctttagcc
     8461 cacatgaaag aggtctatgg ggcaatacaa cgaggcgcaa acggatcatt cgtcctacca
     8521 cgctgttacc aggacaggac aaatcacact gtgccaattt tgtgtcagaa caaagtggaa
     8581 gcaatagacc taagaaaaag gctggaacaa atagttcaga aaatcaacgt tttggggaac
     8641 caaacgcaat aacgagcact catcaaaaac ggtctattta tcctgccaag gcgatgacct
     8701 gagttcaagg cccaggcagt ttctcctaat cgcttgaatt gacagtgatc tagagggaac
     8761 tgctaccaca ttgctaccga tcagtacgat acacagtgtt ccctgaccgc gtgcagaact
     8821 ggggcttccc gtctccctca atcgcgggaa tcaagtgtcc aggtcgacgg tgatgctaat
     8881 ccttttatgg ccgagctatt tcgggggttt ccttttgagc ggttcctttt cagagtgcct
     8941 gttcactggc cggctataaa gcgatccttc tcgatgtaaa gaggcgagtc aacatcttga
     9001 taagccgtag gaactgctgc agcggtgggt gtactcatcc acgtatgagg caggcggact
     9061 atacatttgc aaattgtccc gatccttctg ataatccgta atctggaacc cacgggaatc
     9121 gttaattcag accgttctgc caatctaagc acaccccatg aaagcattac tgccggtcgc
     9181 gctagccata aaaacgacca tcagcgtttt actgatcact tttatcctgt tcgaagccgt
     9241 actgcgcctc tatcacttcg tcaatcccct ttttgttttt ccggacaact cggataatcg
     9301 ctttcgagga aagccctatg gcgatgatta tgatttcacc ctgaactcaa agggcttcaa
     9361 agacattgag tttcgcagcg agaaggtcaa tggaacccgc cgattagttg gaataggcga
     9421 ctcttttgtg ttcggtactg ttccatacaa atataatttt ctgaccgtgt tcgaggagga
     9481 atggaacagg gcgcaaaccg cgtctcctat cgaagtattc aatatgggca taccaagcac
     9541 cagcccacgc caatatctat cagtgctcat taaagacgct ctcccgctcc aaccagatgc
     9601 cgcactcgtg ttcatttata ttggtaacga cttcattgag atttctaaga agccgttgct
     9661 ccagcactca tttgtcatcg cagctggtaa atttgtattc gatctcatcc gccatttaga
     9721 agacggcgtg acgccccaca gtcgtacgtc acagtacaaa gatgatgcag ctacattatc
     9781 ggtggacaag tttctagaga ttgaaacagg tcgcagtgat atctatgcgc tagacagcaa
     9841 gctgctgccc gagcgtctgc ctcgtgtgat gtattttgtg gacgaaataa atagggtgtg
     9901 caaagagaaa gacattgcgt tgtatattgt gttgattcca gacgaggtgc agattgatcc
     9961 aaagttgcag caagatgtcc ttgcggctct tcaattgcca actaacatgt tcgattttga
    10021 cctgccaaac aaacggcttg cacaggagtt acagggtcgc ggcattaagc atctagatct
    10081 tctgccagca ttcagagatg ctagtagaga agaaagaaga ctttatattc ccaggaacac
    10141 ccattggaac attgaaggga accgcctggc agcggctctt ctcattgaat ttctccgtca
    10201 cgaattaagt acgtcgtgag cgcacaaaac ctcacgaaga ggacggacag gatctttccc
    10261 tttttcggca agaggatccc aaatgagtac ccgcctgcct gaaaagccgg cggtctttcc
    10321 acgatcgcat cttgttcaaa gttgcgataa cttgccgcgt ttttcacttc tttgcatcct
    10381 agagatttga tctgattgca atccgttggg tccgtgaaat tcgaaacccg ttgtccaccc
    10441 aagcacttct ctttgtaact tgtgcgtatc cctgtcgttt cgctaactca agtgccgaga
    10501 taggttggcc cttgcgccga ggtggtcaag aagcggcttg caagatggtc atgttgtgga
    10561 tctgccggcg aaagctaaac agaatttgca gaggtgtaaa gaagccgatc caacacgcct
    10621 gaccgggaaa ctagggcaga cccaagcctt ggatgatctt tccagtttca gcatccacac
    10681 gaaggcgcct ggacttattg aaggcatcga tgatctcgat atcataaaag acgcgggtcc
    10741 cagactgccc aagctgggct gccacggctc ttccaggtcc gttcttctcc gcaatttgcg
    10801 cggcttgcgc gagcgtcacc gtggcgtgac gcagccagta gccttttccg tgctcctccg
    10861 cacaagccca caacgttatg gcagacaatc cgatcagcag cttccagagt ccgcgcatgt
    10921 gttatccatt gtgcacagta tcagacgata tggtcggcgt gtatcgctcc tgtatcaggc
    10981 tcgacaagcc gccggcagtc ctgggcttgc agtgttatta gggcacttcg cagctcgggc
    11041 aggaggcaac atggcgagaa gcggtggagt cctcggagtc tacgaaggta ggaccggggc
    11101 gatcgataga gcatcgactt caccgccttt cgtggcactc gccatacgtc tgccatcccg
    11161 cggaggacaa accggaggaa gcctgaaggg aaggagccgc acctcgagat aagattgcag
    11221 tctgtccata actcgtgtgt cttatacggt tcgataagga ttcttggctt gtcatagcag
    11281 gtgtctcagc taaagaccaa tatttccgag aattcgaaaa caatgacgca tagtctgcta
    11341 gagacaaagc tcgtgagttt tcaacctgaa ggcccgataa gcgtgtaatg gcatgttttc
    11401 aattgacgcc agttcataca tgagtccaga atacagctca cttcttacct cagatgccgc
    11461 cggcctcctg gaaggcccga ccgccgacat actgacaagg gcggttgtgc atggcggacc
    11521 ctaaatccat tctcctgatc gacggtagtg cccgggatcg acggtactat ggcagtgtgg
    11581 tccaaggcgc gctccccgat tgccttatct tggaagctga gaacggagag gacgggctta
    11641 aacttttcca gtcgaagtcg attgattgcg tgattctcga acttgcgttg cccgacattc
    11701 atgggaccga tttgcttaaa cagctagccg ttccggcttg cttgcctcga aggccaatta
    11761 tcgtactgac tcgactcttt tatccgacta tcctaacaat cgctagggaa aatggcgcca
    11821 cagcaacgtt cgtcaagaag gtgacttctg aacaagcctt acaggatgct gtgctcgcgg
    11881 ccctctcgac tattcctgct tcatcaagcg ctgagaaagg ttgatgaaag agtagagtcc
    11941 tccaaaacat ttcccacttc tactattacg tggatcatgt acgacactcg ccagatttcc
    12001 tcatcgacga accgaacctt ttcacggcaa aggcaatccg ttccgctgac gtcaaagcat
    12061 aagggtcaat gcgtgctaaa acgtttggct cgtcatcgat ggagagacca catcctgagt
    12121 agcctggggt ggtatccttg gcagtgctgc cgatgccata ggacttcata tcttcgcttc
    12181 cgcttttgat cgtcgaacaa tttcctttca cagccgtcac tggactacta gctctgtcgg
    12241 gcgcgcattc ttacattcca ctcacgagcc aatttttgcg catcagcgat ttagacgatt
    12301 ccctttttac catcgtgata cccatgtgga gtggccaggt tgtacccaca tatgtgcttg
    12361 cacaaatatc ctgagtaaca ccatgcgctg tatgatagag acggccgatc atgaacaccg
    12421 caccgccatg atcagcggcg agactacacc agcataacgc ctctggtcgg agtccattcc
    12481 tcgaatcagc agcacgcgag atggttttca acgtctggag agctatgcgt gaactcactt
    12541 gccgaagaca acgttgcctt tgaaagcggg tgtcggtagt gaaactcctg ccagcttctt
    12601 gcacaagaca aatatccact gtagtccaaa agctggggga accaccggac gcagaataaa
    12661 ccacacgcgt gaccgagttg aattactccg gtttcgagac tggtggtggg agcactaatg
    12721 gttcggtcgg ttgagttgga tgagttgggg ggggtggagt agtgggtcgt tgaagctttt
    12781 caatgacgtc ttgtttcaat ctcgtcggct cttcgacgcg gttttcgcgc tgcagcgaat
    12841 tcagttcggc ggacttgcgc gcattgagat ccaccatgtc cacctcatct ctcaccaggg
    12901 agggagtcag gaacacgagc aaattgagtt tttcagattg ccggctttgg aatcggaaga
    12961 gccaccccaa cagcggaata tctcccaaca gcggaacctt gcgttcactg agggtcaagt
    13021 tatcccgcac taacccgccc acaaccaagg tctgaccgtc cttggctatg gtggtggtct
    13081 ccatagaacg tttggtcgtc gtcggtccca ccgggaccgt cgcaacccct gtgccgatgg
    13141 tctgggcgac ggcttccgca atggcggtga tttcctgctt gatttcgagg cggatcaagt
    13201 cattttccag tacttgtgga gtcaactcga gcgtgacgcc cacgtccttc cgttcaatcg
    13261 tcacgagcgt gccgccggtg atgccctgcg cctggcctgt cggaaagggt ctgttttcac
    13321 ccacgacaat cttggccttc tgattgtcgg ccgccaatac ctgtggagtc gaaagcacgt
    13381 tcgtgtccgt cagactcaga agaagttgta agaacgcccg gatgttgatg gtattgagta
    13441 ccgacactgc tccgcctgtc gaggtccccg ctgcgatacc actgatggtc tgtgcgatcg
    13501 tcgcaatatc ctcaggcgct cggttaatgc tgccgatgcc ttggaactgg ccgctctttc
    13561 cggccgccaa aacctgaacc gggtcggtgc cgatttgccg gaggcgatcg acttgcacct
    13621 ccaaaatcac ggcttcgacg aacacctgct ttcgtcggcc atcgaggtcg cgaatgaccg
    13681 attggagtct cgcgtaggcc gacttcgtcg aactgatgat gatggaatta gtcgccttgt
    13741 cggcaaatac ctggacggga gcttcaaatt cggtcaaggg cctgatcggc ggcctgattc
    13801 cgggcgttgt ctgtgagacc gtctgcgagc gggcgaccaa gttcgttaag accgccgcca
    13861 aatccgtggc attggcgtgt ttcagtttat agacgaagac gccgcgttcc ggtggaagtt
    13921 cgcccatctg cacaatctga gttacaggtc ctcgcggcac aacagccagc cggctgactt
    13981 cgagtgcgga cacgaagagg ctgtaagcct gatccggcgt caccttggtc ggagaaaaca
    14041 ccgtgatctt tccgcccgct ttcttgaccg catcgtctaa gacgaaattt ttccctgtga
    14101 gctcgctgat gaaccgcacg aaaacggaaa gttcgacgtc gttgaaatcc aacgccaccc
    14161 gagaattgcc gttggattgt gccgcaacag acagagggac cagcagtccg aatgtcacgc
    14221 agcacaccca gacccggaga ggtccgcacc acgaacgaga cggaatctgt gggaagaccc
    14281 gcagaaggcg gggtaggcgg gcgccggacc gaaactgtgt catccgctca gtcccgatag
    14341 tggatggagg aaaagcattt ggccagacgg taacacaagg cactgctgga cacaaccaat
    14401 tggggcttcc atactccggc cagaccgggc cgcgatctgt cgcacttgcc cattctcggt
    14461 cctgctgtgc aaatttgaag tggtcttgtc cgattccgtt gtcacgaccc gccgtaactt
    14521 aagagaaaca aagagaaaga gcagtgacag cgtggggcac gatggcatca ggattgcaga
    14581 tctgtcacca gtttccccca ttagtcatcc cggtacgtct ggcggaggcg caccacggct
    14641 gaaaccagcc gtcagagccg gtttgcattg aacctaatct gctaaggtta aaacgtgcgg
    14701 atagacaagc gctcctctca acggaccgat aaaaaggcac cctcacgagt gcataagcgg
    14761 ggtgtcgtcg gatacagcaa gaagaccgcg ctgctcgagg aagcgatcac gcacatgaac
    14821 accggcaagt acggccggtc gtccgctgct ctcaaagaac tgctcgcgct cgatccgcag
    14881 aacaccgaag cccgccgatt gttcgccacc ctccatcttc ggcttgggag tctggtcact
    14941 gccagacaag ccttcgagtc actcgccaac gaagcgattg ggcgccagga ttactggctg
    15001 gccgagtccc tgctgggaga atacttggcc gccggtcctc gttgcgtccc cttcctcgag
    15061 cagctcgcat acgtctatca ggaaaaaggt gacgaattgg cggccgtcgg cgagctcggc
    15121 aaagccattg aaatcctgcg agatgatcca gaccccgaca atccgcagaa agctgcgcaa
    15181 ctctactcga agatcaggga attggcgccc gcaagctcgg tggccatgca actggctccc
    15241 ttgttcgacg tgcagaccgg agagttcctg gtctgcccgt ccgctcagtc acctgcgcac
    15301 gctgatgcct cgtcgtcact tgctgatgca tcggctgcag cccacgtgga tcaccccctt
    15361 cccgaagtga tgccttggga agtgaccgag gaaacgtctc cggttcccga ctctgaagcg
    15421 agcctcgctg catcctacgc ggcaagtgaa gaggcggctc tatccacgtc ctgtgtaatc
    15481 gaagatgtcg gatccctgga ccaaacggtg tcggagcccg tgcagagcac gcctgtcgat
    15541 gaaccaccgc tctcgttcac gctgaggaca gaggagactc tccttgagcc ctctgccgac
    15601 gcttcggaca cgacgacggt gccgagcgaa tcggtcttgc cgcaagcctg gggcggtatg
    15661 ccctcgcaca tgccgtggga gcagatggct gacgcctcga tacggatcat tgaacctgaa
    15721 agtccccctc cagatccggg cttgctcgaa tcgatcctgt cctcattgcg aactgaagat
    15781 actagcgtcg cttctgaggg gccggttgca gaggcaacag cgtctcaagt ggaggccgag
    15841 gagccgatgg cgtcagcacc cgcaccggtc acggatggcg atattgcatt cgtctccctg
    15901 tcgtcaccga tgccttggga gcaagtttcc gatgcggcca tgcagattcc tgcagaggag
    15961 cctacgcctc ttcctcttgt cgaagaccgg ccgttccata tcgcgaccgc tgaacccgag
    16021 ccgtctgctt tatccaccgc gggtgactct tgcccgttcc agccggcgac agaccaaggc
    16081 gcgtctccgt cgtccgactc gaatcctcca gcagattctc cgccgctgcc gtcagcggac
    16141 agcgcaaagc ccgcctcctt ttcatggaat tcagtgtttg ataaggcgtg ggaattcaca
    16201 tcaggaacca tggcgccgag tcccccggtt ccttctcgaa tagatcccga gcctcgtagt
    16261 caagggcagc cgggcgaagc tgcgccgctt ccagtaacag cagatggccc tgtttcggaa
    16321 cagtcgatcg cagtcgaatg ctcgcctcct atagagacgc ccctctcacc actcgagccc
    16381 gcagtgtctg cttcgtcttc ggccgtcgtc gaagaaggcg ccatacatga atacgtcgtc
    16441 gagtcgacgt ctccaccggc agttccgtct gaacattgga acaccggcga ggtggcgata
    16501 caggtgcatc gaccttccaa gaagcacaaa cgttcgagac acggaccgga ggaggctgag
    16561 ccgaaggctt ccccagctgc tgctcccacg acggagtcct tatccgatga actcagtcag
    16621 accaaacggg aatggatatc ggcctccgac gaggcagcgc ccctcattga agagcctgtc
    16681 tcagtgtcgc cagatacccg tccggactgg atgcaggcca cggatgcaat tactttcgat
    16741 aggccggcgc cgccacctgc cgcaacatgg agcgacgcac cggccgtgac ggcgtcccct
    16801 gtagaggtag aggttgctcc atctgccgcc gcctcagccg tcgacatgtt gtttgcacgg
    16861 agcggaccca tcgatgacgc ccgtccgcat gtgtctttgt ggtcgaagcc ggctcctcgt
    16921 ttgatcgctc gcctgcatcg gatccggatc agcatctggt cgttcatctg gtcatgcttt
    16981 tccacgacgc ggtctctcac ctttctcgtg ttgatgactg ccgtggctgt cgtggtggtg
    17041 gtcgccgcag gccttgctgc gatagggttg acatggatgg ccatggaaga tgcgccaagt
    17101 ccgctgtatc agaacttgac cattagcccg ccacgaacga tcacggatgc gaaaaagaac
    17161 ggctatcttc tgttgttggg atttgacgcc ccggcaggat tggatcctct gcaagtcggg
    17221 tacgagcgca aacctggcct gcaggacaag acggcggcac agctctgctc ggcaggcgac
    17281 gaggcgaaag acacaggatc agccggcgct tcagggcagg tcgtcagagg atgggtccgc
    17341 agcggtgact tcttgtcgca agtcaaagga cagagcggga gcattcgctc gttcgtctcg
    17401 cgtgaatccg tcacgcttgc acgctaccag cagtggctcg ccatgccgtt cgatgattgg
    17461 ggatttggac aatcgctgag ccccaattgc tcacgggtct tgctggcgca ccgcctcttc
    17521 gtgctggaag gattcaatca agatctttcc agcggaatcg atcgactgga aaccgacatg
    17581 cagtcgtggc gagccgcgct gggacaatcc aagaccttag ccatgaaaat gcttgccgtc
    17641 acggcggtgc aggatgatgc ctcggtagcc tcgggattgt tgagccgcgc agagttggac
    17701 ggagcggccc tagcgcggct gacgaagatg gtgcgccctc tcgatcaggc tgaactctcg
    17761 gtacgctggc ccatgcacag ccaatttgtg tgggccacca aatcggtttc ggccgaactc
    17821 aaagacgacc agactcgaga ccgtccatgg catgcgacca tggcgtcggc aatgcgactg
    17881 cccattcagc gcagagcgaa tgcctacgcc gaatattatg aagcggcgaa caaggcggtg
    17941 gccggaggcc gttacacgaa cctcccgaaa ctctcatcct ttgtacggac gcctccttcg
    18001 ggggtaatgg attatctcgt caatccggtt gagcacattg tcggagtcga gcctcttcct
    18061 tcatgggacc cgtacgtcat gcggatagtc gaggccgatg ccgaactgcg tttggccgga
    18121 ctccaggcct ggattcggag aggtccgcaa gaaggcgatg ttctcacgcg gttggccaaa
    18181 gccggccaag cctcctacga ccctttcacc ggcctgccaa tgctggtcaa ccagcggaaa
    18241 ggactcatct acagcgtcgg gcgcgacggc aaggatcaag aaggggacaa cactcgtgac
    18301 ctggccgtac ctattcccat cgtttcatct tcagactcca gacgggctcc gctttctcct
    18361 ccggttcgat aaacacaacc tatctgcagc ctggttcacc tcggtgcgtc cgttcccggc
    18421 gtcggacggg cggatgtacg cttgacaggg tccggggtat tccacacaat cctgcggagc
    18481 cgttccgcct ccggagggat accatgaaac cacattcgac ctcgcgcctc cggcaaggag
    18541 cccattcaac cggaccgata cccacccaaa ttgtctcgaa cgaggagttc accccatggc
    18601 cacgtacgcc ggaacaagct ttagtcgagg aggccgtggt cagaacgagc cgcaaggcgg
    18661 ccgtgcgcct cggcatgact cgccggcatt ttctcaagac ctcgggcggg atggcggcgg
    18721 cattgctcgc cctgaattcg gtgttcggcc gtttcttcga agtcttcgag gccgagctgt
    18781 tcgagccggc agctttcgcc gagcaacagg gcgcttccta cttcgtcttc gatgtccaga
    18841 cccattatgt cagcaccggc tatgatccgt ctgattcgga agtgcgccgt cgcggggcgg
    18901 tgtctaaaga ggctctgctg ggattgaggc ggagagcgcg tgactcggga ctcaatcctc
    18961 gactcacggc cgaccgtggc acgatcgatg atctctcttg gcaaaacttc gtcaaggagg
    19021 tgttccttga cagcgaaacg tcggtggggc tgatcagcac ccctccagga ccctatccgc
    19081 aagaagcggt ggtgccgcca aaggaaatgg cgcatatcag agatgagctc aatcgcctcg
    19141 ccggatccca acggatgctc gcgcacgggt tggccacacc gcaattgggt gcgacggatt
    19201 tggagttcat ggcgcagcaa gcggagcaac tgaaaatcga tgcctggaaa tgctataccg
    19261 gctcgtgtcc gagaggcttc gaccacggct ggtggatgga cgatgagaag atcgcgtacc
    19321 cgatgctgga acaggcaaga aggctgcatg tgccacgtgt gtgcgtgcat aagggattac
    19381 cgctcggtcc ggtccctgat tacaatcacc cgagggatct catcaaggct gccaaggact
    19441 ttccggacat cgattttctc gtctaccatt ccggcatgaa gagcgctcaa tccattgatg
    19501 aggtgttcgg caagaccggc gaaataccct ggacgactga attctgtcga atgaagcaag
    19561 cgcatccggg cctgaaaaac atttatatgg agcttggctc gaccttcggt cagttggtca
    19621 ccagtcatcc actggtctgt gcgcacttga taggccagat attgcaagcg ttcggtgacg
    19681 atcacgtgct gtggggaacc gactcgatct ggtatggaac gcctcagtgg caaatcgaag
    19741 cgtttcgccg cttccagatg ccggaccact tgatcgaaca gcatcactac ccgtcgctca
    19801 cacgagaggt aaaggcgaag gttttcggat tgaatgccgc acgtctcttc gctgtggatc
    19861 cggcggcaac aaggcagggc attcccaaag actacgtcgg ccggcttcgt ctgagttatc
    19921 tggaggaggg accggaaccc agtcacaggt tgtacggatg ggtggcgacg taggaacacg
    19981 ggtgggatga ggagaaagag atgaagcacg aaattttgta tccaggggct ttcccgatgg
    20041 tgcgggtgga attggcagcc ggagagacga tcaaagccga gtcgggtgca atggtcgcct
    20101 cttccccgac gatcgacatc gaaagcaaaa tggaaggtgg ttttctcggc gcactctccc
    20161 ggaagctgct caccggcgaa aaattcttct ttcagacgtt gcgggccagt cgtggcgcgg
    20221 gggaagtgtt gctcgcgccg accgtcccgg gtgaaatagt tgtcatggaa ctggacggtc
    20281 tcaatgaata catggtgcaa aaagacgggt ttctcgccgg ctccgaatcg atcaagatcg
    20341 agagtcagat gcaaagcctc agccgaggtc tccttggtgg agagggattt ttcattctga
    20401 aagtcagcgg caaggggtcg ttgattttaa atagcttcgg cgccatccat aagatcgaac
    20461 tgaaacctga tcaggaatat atcgtcgata acagccatct cgttgcctgg tcggggacca
    20521 cctcctatca catagagcgc gcggcttcgg gctgggtggc gagcttcaca tcgggcgaag
    20581 gatttgtttg ccgttttcgc gggccggggg tcgtgtacat tcaaagccgt aatcccggta
    20641 gctttggcgc gtgggtgcgc caatttattc cagtgagcga ataacttggg ttcagcaa
//
LOCUS       DASTQY010000339         8301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1702177, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000339 DASTQY010000000
VERSION     DASTQY010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8301
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702177"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..514)
                     /locus_tag="VFR82_11170"
     CDS             complement(68..514)
                     /locus_tag="VFR82_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEU4502200.1"
                     /translation="MAIVHDDVGRRRFLSQAVMGFGMLFGIGTLGFRFMQFLLPTRTD
                     KQPETVLIGAETRIPLGDAVPMDLGGQKILVLKTQDGVAAFSRRCTDLGCLVSWNKER
                     EQFVCPCHQGTFDKNGVNIAGPPPRPLDRFQIIKRGEQLYVNIASA"
     gene            complement(517..1680)
                     /locus_tag="VFR82_11175"
     CDS             complement(517..1680)
                     /locus_tag="VFR82_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEU4502201.1"
                     /translation="MTQPADQILWLLISTTLLALVVPGVALFYGGMVRRKNVINTMAL
                     PFLALAVVSLAWGLVGNGLAVSGATGGSTGRLDALLSLHRGMMASVALALVAGGVVER
                     IRFSFFLIFGLCWVVAVYVPLAQWLWGGGWLTRLGCLDFAGGAVIHISAGVTALVMAI
                     LIGPRRGYGRSDMMPNHLPFSFCGAAFMWVGWFGFTGASADLSMAGVTNAFVTIQLSA
                     VAATLAWMTAEWLQRDKPTALGAMSGAVAGLVAIAPGAGYVSPLSAIVIGVGAGGLCY
                     MVVNYVKVILGYDDSLDVFGMHGVGGTWGMIATGLFASTEINASGSDGLFYGYPYQFF
                     VQVVAALAGWAVAGGVTWLLYHVLTWFIAPRVDEEAESMGLDLDQHGEKGYTG"
     gene            complement(1677..1904)
                     /locus_tag="VFR82_11180"
     CDS             complement(1677..1904)
                     /locus_tag="VFR82_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502202.1"
                     /translation="MIMTQEMRPDTSACKRRLLEVLRRDGPQTLDALCELPEFTWAQV
                     LMAVDHLSRSRDISLELIGPREYQVSLAVRK"
     gene            complement(1916..3238)
                     /locus_tag="VFR82_11185"
     CDS             complement(1916..3238)
                     /locus_tag="VFR82_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEU4502203.1"
                     /translation="MKTRIGSREWMSGLAVCAGIIVPNECSAETAAATVISGADTAWV
                     LLSAALVLAMIVPGLALFYGGLVRSKNVLATIMHSFVILCLVSLLWVLIGYSLAFGPD
                     VNGVIGSLAWAGLNGVGLTPHAVYGPTIPHQAFMVFQLMFAAITPALITGAFAERMKF
                     SALLCFSVLWSLAVYCPIAHWVWGGGWLAGLGALDFAGGAVVHISSGISALVCAIVLG
                     ARQGYGTDYMAPHNLPMVLLGTGLLWVGWFGFNAGSALGANETAVVAFVATHTAAVTA
                     ALSWMAVEWWHRKTPTVLGLASGAVAGLAMVTPGAGYVEPSSALFMGMAAGILSYLAI
                     MKKARFGYDDSLDVVGIHGVAGIGGILLTGLLATKAVNAGGVDGLLHGNAGFFAVQGL
                     TVIVVGLFSAGGTWAILKIVDRAVGLRVPAEEERMGLDISQHNERAYS"
     gene            complement(3434..3874)
                     /gene="cynS"
                     /locus_tag="VFR82_11190"
     CDS             complement(3434..3874)
                     /gene="cynS"
                     /locus_tag="VFR82_11190"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HEU4502204.1"
                     /translation="MEKDEIRKAIKAKRLAKKVTIAEVAKAVGRNPTFVGAVLQGTHK
                     LPPEEAKKVGDLLGLDAELTTSLSKFPVRTDFPNTTDPFKYRLMEIIGVYGDSMREQA
                     NEMFGDGIMSAIDFTLDMEKVTGSQGEARCKITLNGKWLEYKTF"
     gene            complement(3912..4772)
                     /locus_tag="VFR82_11195"
     CDS             complement(3912..4772)
                     /locus_tag="VFR82_11195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01184.1"
                     /note="This model describes the ATP binding subunits of
                     ATP-binding cassette (ABC) transporters for nitrate
                     transport, or for bicarbonate transport, in bacteria and
                     archaea; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter ATP-binding protein"
                     /protein_id="HEU4502205.1"
                     /translation="MSFLLIDHVTKYFSNPSGAGQVCIFKDATIKIEKGEFVTCIGHS
                     GCGKSTLLNIIAGLETPTEGGIILNGREASGPGLDRMVVFQNFALMPWMTVFENIALA
                     VRSAYPDWSREQVSAHVTKYIQLVGLKGAENKRPVALSGGMKQRVGLARAFSIEPKVL
                     LLDEPFAQIDALTRGVIQEELIQMWNNSRNTVFMVTHDVDEAILLSDRILLMTNGPEA
                     RIAEEVHVSISRPRSRETIIEHPHYYKIRNHIIHFLVRHAKHGGEGMMPGTNGTPVVV
                     NFEEDSLVTH"
     gene            complement(4776..5831)
                     /locus_tag="VFR82_11200"
     CDS             complement(4776..5831)
                     /locus_tag="VFR82_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU4502206.1"
                     /translation="MESTNRTMLPELTRTARVEASLKLPIHLFQAGIASTKRWLTRWR
                     SASHVQNQRGGGMAAQSQGNPWYMAGFVLVLFLLSWHFFTLRSSFDQRGLTEEQLQMM
                     EFNGDIVRAPDGSYAWNPEKEKVSGIPGPLAVLAKAREELSEAFVKKGTNDHGIAYLV
                     FYTVSRFAGGFLAASVIAIMLGVILGLNKVLFRAANPFIQIMKPISPLAWMPLLLYTV
                     KDPKWTAVLVVFMAAVWPTLATTAFGVSSLRKEYLHVAAIVQLSWVERLFKVILPGAA
                     PTIVNGLRISFGSALVAVVPAEMLLGELGVGYLSWIEWNNLDIAGVIFAILVVGVVGV
                     ILDSGFNKLAGYVTYQE"
     gene            complement(5841..7148)
                     /locus_tag="VFR82_11205"
     CDS             complement(5841..7148)
                     /locus_tag="VFR82_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU4502207.1"
                     /translation="MKDEQLSEDETNGTLSSEQYFERTLDQAVTAAVVEAIGPTRRQV
                     LLGLGAATLSALVADIFPLRTLKAFAADPVGKPEKKDVSIGFIPITCGTPIIMAEPLG
                     FYKKHGLNASVKRAAGWAMIRDWTVNKEVDAAHMLTPMPLAITLGAGSVPTPIYMPAV
                     ENINGQAITLHVKHKNVKTASEMKGFRFCVPFDFSMHNYLLRYFLAEGGVHPDKDVQI
                     RVVPPPEMVANLKAGNVDGYLGPDPFNQRAVYENVGFIYKLSREIWDRHPCCAFTVTK
                     EFATTYPNTFLAMWRAIVDATHYASDPAHRKEIASAIAPTNYLNQPVTVLEQVLTGTY
                     ADGLGNIKKEPNRIDFDPYPWHSMAIWILTQMKRWGHLKGDVNYKSVAEQVYRAADCD
                     RIAKELGYATHQSTTTRHVIMGKEFDSNSADAYVKSFKIHSMA"
     gene            complement(7571..>8301)
                     /gene="glnA"
                     /locus_tag="VFR82_11210"
     CDS             complement(7571..>8301)
                     /gene="glnA"
                     /locus_tag="VFR82_11210"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247938.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU4502208.1"
                     /translation="LRTADKMMMYKYIVKNVARRHGKTVTFMPKPIFGDNGSGMHTHQ
                     SIWKEGKPLFAGKEYAGVSQMCLHYIGGILKHAPALAAITNPTTNSYKRLTPGFEAPV
                     LLAYSSRNRSAGIRIPMYSPSPKAKRIEVRFPDPGANPYLAFSAMLMAGLDGIENKIN
                     PGEPAEKDLYDLEAKEAAKIRTMPGSLDEALSNLEKDHKFLLKGGVFTEDLIEAWIGY
                     KRSKEVDPMRLRPHPFEFFLYYDV"
BASE COUNT         1771 a         2614 c         2182 g         1734 t
ORIGIN      
        1 tcgatccacg aggtcttgcc ggcgctcttc gggtcaatgt tttgtgtggt cgttgagtcc
       61 atttcgctta tgcacttgcg atattgacgt acaactgctc tccccgtttg atgatctgaa
      121 accggtcgag cggccggggg ggtggaccgg caatgttgac gccgttcttg tcgaaggtgc
      181 cttgatggca gggacagacg aattgttcac gttctttgtt ccaggacacc aggcacccca
      241 gatccgtgca gcgccgggaa aacgctgcca cgccatcctg cgtcttgagc acgagtattt
      301 tttggccccc gagatccatt ggcaccgcat cgccgagcgg aatgcgtgtc tcagcgccga
      361 tcaacaccgt ctccggctgc ttgtcggtcc tggtgggcag cagaaactgc ataaaacgga
      421 accccagcgt gccgataccg aacagcatcc cgaaccccat gacggcctga ctgagaaaac
      481 gccgacggcc cacatcgtca tgaacaatgg ccatatctac cccgtgtaac ctttctcgcc
      541 atgctgatcg aggtccagtc ccatcgactc cgcttcctcg tcgacacgcg gtgctataaa
      601 ccacgtgaga acatgataga gcagccacgt cactccaccc gcaacggccc atcccgccag
      661 cgcagccacc acttggacaa agaattgata agggtatcca tagaacaacc cgtcgctgcc
      721 gctcgcattg atctcagtgg acgcaaacag tccggtggcg atcattcccc aggtgcctcc
      781 gaccccgtgc atcccgaaga cgtccaacga atcgtcgtaa cccaggatga ccttgacata
      841 attgaccacc atgtagcaga ggcctccggc tccgacgccg atgacgatcg ccgacaaagg
      901 actcacatag cccgcccccg gagcgattgc gaccaacccc gcgacggccc cgctcatggc
      961 ccccaacgcg gtcggcttgt cgcgttgcag ccattcggcc gtcatccatg cgagcgtcgc
     1021 agcaaccgcc gacaactgaa tggtcacgaa ggcattcgtc acgcccgcca tcgaaagatc
     1081 tgcggaggcg ccggtgaatc cgaaccagcc cacccacatg aacgctgccc cgcagaagga
     1141 aaacggcagg tgattcggca tcatgtcgga tcgtccatac cctctccgcg gtccgatcag
     1201 gattgccatt accagcgcgg tgacgccggc gctgatgtgg atcacggcgc cgccggcgaa
     1261 atccaggcat ccgagcctgg tcaaccaccc gcctccccac aaccattgcg ccaacggcac
     1321 gtacaccgct acgacccagc acaatccgaa aattagaaag aatgaaaagc gaatacgttc
     1381 aacgacccct cctgccacta gggccagcgc cacggacgcc atcatccccc gatgaagcga
     1441 gagcaatgca tctaaacgtc ctgttgagcc cccggttgcc cccgataccg ccagaccatt
     1501 tccgacgagg ccccaagcca gcgacaccac cgccagtgca aggaatggca gcgccatcgt
     1561 gttgatgacg tttttccttc tcaccatgcc gccgtaaaag agcgcgactc ctggcacgac
     1621 cagcgccaac agcgtcgtcg agatcaacag ccagaggatc tgatcggccg gttgcgtcat
     1681 ttgcgaactg ccaacgagac ttggtattca cggggtccga tgagctcaag cgagatgtcg
     1741 cgggaccggc tcaaatgatc gacggccatc aggacctgcg cccaggtgaa ttccggcagc
     1801 tcgcacaagg catcgagcgt ctgcggtcca tctcggcgca gcacctccag cagccgccgc
     1861 ttgcaggccg acgtgtcagg tcgcatttct tgggtcatga tcatgccttc ctcctttatg
     1921 aatacgcccg ctcattgtgt tgactgatgt ccaatcccat tcgctcttct tccgccggga
     1981 cacgtaatcc caccgcccga tccacgattt ttagaatggc ccaggtaccg cccgccgaga
     2041 aaagcccgac aacaatgacg gtcaacccct gcacggcaaa aaagccggca ttgccgtgca
     2101 acaggccatc gacaccacct gcgttgaccg ccttcgtcgc aagaagaccc gtgagcagga
     2161 tgccgcctat cccggccacc ccgtggatgc cgaccacgtc caacgaatcg tcatagccga
     2221 atcgtgcctt cttcatgatt gcgagatagg acaggattcc ggcggccatc cccatgaaga
     2281 gcgcggagga cggctccaca tatcccgctc caggcgtgac catggccaaa ccggcaacag
     2341 cgccgctcgc aagacccagg accgtgggag ttttccggtg ccaccattcg actgccatcc
     2401 agctcagtgc ggccgtgacc gccgccgtat gcgtcgccac aaatgccacg acagccgttt
     2461 catttgcccc tagggcgctg ccggcattga acccgaacca cccgacccac aacaggcccg
     2521 tgcccaacag gaccatcggc agattgtgcg gcgccatata gtctgtgccg tatccctgcc
     2581 gggcgcctaa gacgattgca cagaccaatg cgctgatacc ggagctgatg tgcacaaccg
     2641 cgccgccggc aaaatccaac gcaccgagcc ctgccagcca tcctccgccc cagacccaat
     2701 gcgcaatggg acagtacacg gcaagagacc acaggacgga gaagcacagc agcgcgctga
     2761 acttcatccg ctccgcaaag gcaccggtga tgagcgctgg tgtaattgcc gcaaacatca
     2821 actgaaagac catgaaggct tggtgcggga tggtgggacc atacacggca tgcggcgtta
     2881 atccgacgcc gttgagtccg gcccaggcga gactgccaat cactccgttc acatccggcc
     2941 caaatgcgag actgtagccg ataagcaccc acagaaggct tacgagacaa agaatgacaa
     3001 agctgtgcat gatcgtagcc aggacgttct tactgcgtac gagcccgcca tagaacagcg
     3061 ccaggcctgg gacgatcatg gccaggacga gggcggccga aagcaggacc cacgccgtgt
     3121 ccgctccgct aatcaccgtc gccgctgctg tctccgccga acattcattt ggaacgatga
     3181 tgccggcgca gacagcaaga ccgctcatcc attcgcgact cccgatccgt gtcttcatac
     3241 gtcgctcgcc tctccttacc atctcgctgc ccactgcaaa cacaaaggcg tccgccattc
     3301 ttcgggaatg acggacgcct ttgtccgttg acgtttgcac cggacggact caagacgaca
     3361 ttgtctcaat ccgtccgcaa cgcagaaatt gtcgatccga tgtcttcagt tcctcctgtc
     3421 cgccatcttc atcttaaaac gtcttgtact cgagccactt gccgttcagc gtgatcttgc
     3481 agcgcgcttc cccttggctt cccgtaactt tttccatatc gagcgtgaaa tcgatcgcgc
     3541 tcatgattcc gtcgccgaac atttcattcg cctgctcgcg catcgaatca ccgtacacac
     3601 cgataatctc catcagacgg tatttgaacg gatcggttgt gttcgggaaa tccgtccgaa
     3661 cgggaaactt gcttaacgat gtcgtcaatt ccgcatccaa tcccaggaga tcaccgacct
     3721 ttttggcttc ctcgggcggc agcttgtgcg tcccttgtag aaccgctcct acgaatgttg
     3781 gattccgacc gacggctttt gccacctcgg cgatcgtgac ttttttggca agtcgcttgg
     3841 ctttgatcgc cttccgaatc tcgtccttct ccatgcgccc ctcctgtgtg tgcaatcgag
     3901 cgtcgagccc ttcagtgagt tacgagagaa tcttcttcga aattgaccac cacgggggtg
     3961 ccattggtgc caggcatcat accctcgccg ccatgtttgg catgtcggac gaggaagtgg
     4021 atgatatggt ttctgatttt gtaataatgc ggatgttcaa tgatcgtttc tctcgatcgc
     4081 ggacgcgaaa tcgacacgtg cacttcctcg gcaatgcgcg cctcaggtcc gttggtcata
     4141 agcaggattc gatcggacag gaggatcgcc tcgtccacgt cgtgggtgac catgaagacg
     4201 gtatttctcg agttattcca catttgaatc aattcttcct gaatgactcc acgcgtcagg
     4261 gcgtcgatct gcgcgaacgg ctcgtccagc agcagaactt taggctcaat ggaaaatgcc
     4321 ctggcgagac ccacgcgctg tttcattcca cccgataacg ccacgggccg cttgttctcc
     4381 gctcccttga ggcccaccaa ctgaatgtac ttggtcacgt gggcgctcac ctgctcacgg
     4441 ctccagtcgg gataggcgga gcggacggcc aaggcaatgt tctcgaagac ggtcatccac
     4501 ggcatgagcg caaaattttg gaacaccacc atgcgatcga ggcccggtcc gctcgcctca
     4561 cggccattga ggataattcc gccttccgtc ggcgtctcca acccggcgat gatattgagc
     4621 aaggtgctct tgccgcagcc ggaatgcccg atgcaggtca cgaactcccc tttttcgatc
     4681 ttgattgtgg catctttaaa gatgcacacc tgccctgccc cggacgggtt cgagaaatat
     4741 ttggtcacgt ggtcgatgag caaaaatgac atggattact cctgataggt gacatatccg
     4801 gccagtttat tgaaaccgga atcgaggatg acgcccacga ctcccacgac caggatggcg
     4861 aaaatcacgc ctgcaatgtc gaggttgttc cattcgatcc aactcaaata tccgacaccg
     4921 agttcgccaa gcagcatttc tgcaggcacc acggccacca gtgcactgcc gaacgagatt
     4981 cgcagcccgt tcacgatagt cggagcggct cccggcagga tgactttaaa cagacgctcg
     5041 acccacgaca gctgcacgat cgccgcaacg tgcagatact cttttctcaa ggaactgacc
     5101 ccgaacgccg tcgttgccag ggtgggccat accgccgcca tgaagacgac cagcacggcg
     5161 gtccacttgg gatccttgac cgtgtagagc aggagcggca tccaggccaa tggagaaatc
     5221 ggcttcatga tctggatgaa tggattcgcg gcccggaaga gcaccttgtt gaggcccagg
     5281 atgacaccga gcatgatggc gatgacggac gccgccagaa atcccccggc gaaccgcgac
     5341 acggtgtaaa acaccaaata ggcgatccca tgatcgttcg tgcctttttt gacaaaagcc
     5401 tcggacagtt cctcgcgcgc ctttgcgagc acggccaaag gcccgggaat cccgctgacc
     5461 ttctccttct ccggattcca ggcatagctc ccatcgggag ctcgcacgat gtcgccgtta
     5521 aattccatca tctggagttg ctcttcggtc agccccctct gatcaaaaga tgaccgcaag
     5581 gtgaagaaat gccagctgag caggaacagc acgagcacga atcctgccat ataccacggg
     5641 tttccctgcg actgagcagc catccctcct cctcgctgat tctgaacgtg tgaggcgctt
     5701 cgccaccgcg tcagccatcg cttggtcgaa gcgatccccg cctggaacaa gtggatcgga
     5761 agcttcagcg acgcttcgac ccgtgcggtc cgtgtcagct caggcaacat cgtgcgattg
     5821 gtcgattcca ttatctctcc tcaggccata ctatgaattt tgaaactctt gacgtaggca
     5881 tccgcactgt tggaatcaaa ttccttcccc atgatcacat gtctggtggt cgtcgattga
     5941 tgggtcgcgt agccgagttc tttggcgatg cggtcgcaat ccgctgcccg atagacctgc
     6001 tcggccaccg acttatagtt cacgtcccct ttcaagtgtc cccaccgttt catttgagtg
     6061 aggatccata tcgccatcga atgccaggga tacggatcga agtcgatgcg gttcggttct
     6121 tttttaatgt tacccagccc atccgcatag gtgcccgtca acacctgctc cagcacggtg
     6181 accggttgat tcaggtagtt cgtcggcgcg atcgccgacg caatttcctt ccggtgcgcg
     6241 ggatcggacg cataatgcgt ggcgtcgacg atcgcccgcc acatcgcgag aaacgtgttg
     6301 ggatacgtcg tcgcgaattc tttggtcacc gtaaaagcgc agcaaggatg ccggtcccaa
     6361 atctcccgcg ataatttgta aatgaacccg acattttcat agaccgctcg ttggttgaac
     6421 ggatcgggac ccagataacc gtcgacgttg ccggccttga gattggcgac catctcgggc
     6481 ggcggaacga cccgaatctg cacgtcctta tcaggatgga cgccgccttc cgcgaggaaa
     6541 taccgtagga ggtagttatg catggaaaaa tcgaacggga cgcaaaaccg aaagcccttc
     6601 atctcggacg ccgttttcac gttcttgtgt ttgacgtgca aggtgatggc ttggccgttg
     6661 atattctcaa cagcgggcat gtagatcggc gtggggacag atcctgcacc caaggtgatg
     6721 gccagcggca tgggagtcag catgtgcgcg gcatccactt ccttgttcac ggtccaatcg
     6781 cggatcatcg cccagccggc cgcacgttta actgaagcgt tcagcccgtg ctttttgtaa
     6841 aaacccaagg gctcggccat gatgatcggc gtgccgcagg tgatgggaat gaagccgatg
     6901 ctcacatcct tcttttcggg cttgccgaca ggatccgccg caaacgcttt caacgtcctc
     6961 aaagggaata tgtcagcgac caacgcgctc agcgtcgcgg cgcccaggcc gaggaggact
     7021 tggcggcggg tcggtccgat ggcctcgacc accgcagcgg tcacggcctg atccaatgtc
     7081 cgctcgaagt actgttcgga cgacaacgtc ccgtttgtct cgtcctcgct caactgttcg
     7141 tccttcaagg ctcgctcctt tcgtcacgac cctttaccgc cgtcaagacc aaaacaaaag
     7201 gcgtccctct ctcgctcgag agaaggacgc ctttgtccgt cgtcagcagg tctacagcga
     7261 cctgcccatc gtctatttca gaaccaaaaa ggcgtccgcc attctttgga gaatgacgga
     7321 cgcctttgtc cgttgcctaa cttttcaagc cgtagcaggc gccattgcct tgcttagctt
     7381 gtaggaactt gtagcatggt tgttccgttg ccgtcaaccc tccgcgccgg atgctgaaaa
     7441 atcccgccag cttcgttctc ggatcgctta gagactcaac gtacggctca aagtacgctt
     7501 cgcctctgcg ctccctgcgg ccttgctgga cgggcttttt caccatcctg caaagctggg
     7561 tacccgtctc ttagacgtcg tagtacagga aaaactcgaa cggatgcggc cgtaaccgca
     7621 tgggatcgac ctctttgctc cgtttgtagc cgatccaggc ctcgatcaaa tcctccgtaa
     7681 atacaccgcc tttcaacagg aatttgtggt ccttttccag attgctcaac gcttcgtcca
     7741 gactgcccgg catcgtccgg attttggcgg cttccttggc ttccagatcg tacaggtctt
     7801 tctccgctgg ttcaccgggg ttgatcttgt tctcgatgcc gtccaggccg gccatcaaca
     7861 tggccgaaaa cgcgagatag ggattggcgc ccggatcggg gaaccggacc tcgatacgct
     7921 ttgccttggg actcggtgaa tacatcggaa tgcggatgcc cgcggaacgg ttgcggcttg
     7981 agtaagccag caagaccggg gcttcaaatc ctggcgtgag ccgcttgtac gaattcgtcg
     8041 tcggatttgt gatcgccgcc aatgcgggcg cgtgcttcag aatgccgccg atgtagtgca
     8101 ggcacatctg cgagacgccc gcgtactctt tccccgcaaa gagcggcttc ccctctttcc
     8161 agatactctg gtgcgtgtgc atgccgctgc cgttgtctcc aaagatcggc ttcggcatga
     8221 aggtgaccgt tttaccgtga cggcgtgcca cgttctttac gatgtacttg tacatcatca
     8281 tcttatcggc cgtgcgcagc a
//
LOCUS       DASTQY010000340         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1681342, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000340 DASTQY010000000
VERSION     DASTQY010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681342"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VFR82_11215"
     CDS             <1..1075
                     /locus_tag="VFR82_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502209.1"
                     /translation="DALYVLRHGEATFRRFTAADGLHVQTYTDPANGQQTVSNITAMA
                     GGISNEVFVGYRGYEAPIPPPAPPPCCVANADFSDPRWHLGQADKVTLDPGGGIQVRR
                     YEFLCDITVNCWEERSARRMIFGHNGTAAGHLFIGFDHGVAHVFNDVFGDHTHIVTFW
                     HYADGRNVMRMGEHYGLALYPNGEMIDGTGYGIGSHEWNADPRAWVNGRFVWGFTTYG
                     PAEPYNNGEHDLIVPEGYREDNRGAAVTPDGTAWWVSLHTGLSSYNHTIAQGNTSLIN
                     HYTAVAGLPAAGLMDIAADPDGSLWIVDNSGRLLRFTPSNQTVQVWPGVSSVRRIVMD
                     TTVTPRALYVSMGSQGLAVIRAR"
     gene            1300..1611
                     /locus_tag="VFR82_11220"
     CDS             1300..1611
                     /locus_tag="VFR82_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502210.1"
                     /translation="MIRYLMLLMIMITLSNCTSVKYNETDCGSLPSDTFWSGNVALQC
                     QEYRRLQAETAYRTEVANMLKSYRECVNKHSGSAAEAKEHCSAYTQLVNDPSSVSRPQ
                     K"
     gene            2012..>2724
                     /locus_tag="VFR82_11225"
     CDS             2012..>2724
                     /locus_tag="VFR82_11225"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HEU4502211.1"
                     /translation="MARTAFLVRDDMALRPPSRHLWLYHPRLVPLKDGESSVRMPPGE
                     LAYEDDAGHLRFRNPYTGLRDQPLLHRDTARAASSDPNIYDIGPPPKHTIKTFAGLTA
                     MSLMGIGVYALGPSSFTGASKEGEDVWGEARDHFKEAWTKPPVFDSDRAVVNYLGHPF
                     FGAQFYLSQRNYGESPLYSFLFSTFTSTCFEYFIESWSERPSITDLIVTPIVGSILGE
                     LVYQATRHMRKDGFTQAEK"
BASE COUNT          596 a          789 c          737 g          602 t
ORIGIN      
        1 ggatgccctc tacgtcctcc ggcacggaga agcgacgttc aggcggttta ccgcggctga
       61 cggtctccac gtccaaacct ataccgatcc ggccaatggc cagcaaacgg tgagcaacat
      121 caccgcgatg gcgggcggaa tctccaatga ggtgttcgtc ggctacagag gttatgaagc
      181 acctattccg cctccggcgc caccaccctg ctgtgtcgca aatgcggatt tcagcgatcc
      241 tcgttggcac ctcggccagg cggacaaggt gacgctcgat cccggcgggg ggattcaagt
      301 tcggcggtat gagtttctct gcgatatcac cgtcaattgc tgggaagaac gctctgcacg
      361 gcggatgatc tttgggcata atggtaccgc agctggacat ttgttcattg gattcgatca
      421 tggcgttgca cacgtgttca acgacgtgtt cggggatcac acgcatatcg tgacattctg
      481 gcattacgcc gacggcagaa acgtcatgcg aatgggcgag cactatggtc tggctctgta
      541 tccgaatggt gaaatgatcg acggcaccgg atatggcatc ggttcacatg agtggaatgc
      601 tgatccgaga gcctgggtta acggtcggtt tgtttggggc ttcaccacct atgggccagc
      661 cgagccgtat aacaatggag aacatgattt gatcgtcccg gaaggctacc gcgaagataa
      721 ccggggtgcg gcagtgacgc cggatggcac ggcatggtgg gtcagtcttc acacgggatt
      781 atcgagctac aatcacacga tcgcccaagg aaatacctcg ttgataaacc actacacggc
      841 tgtggcggga ttgcctgctg ccggcctcat ggacatcgct gcggatccgg atggatcgct
      901 ttggatcgtc gataacagcg gacgcttgtt gcgcttcact ccgtcgaatc aaactgtgca
      961 ggtctggccg ggagtttcca gcgtgcgccg catcgtgatg gatacgaccg tcactcctcg
     1021 tgccctatat gtctcaatgg gcagccaagg tttggcggta atccgggcaa gataatacca
     1081 tcctcatcgt cctgctctct acgacgcagg tggtagtctt tatcgacgac tacctgctga
     1141 ctcgcttgct caaagaccgt cctccgtatt tttctccgcc atcacgtgtg atgttcatct
     1201 tcctcacaac cgttgaccct cttattgctt gcatcgcgtt attctctctg gtacaagaac
     1261 ctacaaaaaa accggcaccc ctattgaaag aaggtgtaca tgattcggta tctcatgctg
     1321 ttgatgatca tgattaccct cagcaattgc accagcgtca aatataatga aacagactgt
     1381 ggcagccttc caagcgacac cttctggtcc ggcaacgtcg ccctccaatg ccaagagtat
     1441 cgccgtcttc aagcagaaac agcctatcga actgaagtgg cgaacatgct gaagagctac
     1501 cgggagtgcg tcaacaagca tagcggttca gcggctgaag ccaaagagca ttgttcagct
     1561 tacacacaac tggtgaacga cccttcttct gtatctcgcc cacagaaata accggaaggg
     1621 aaaggttttt catcccatac gcattttgat gccggctaca cacgcgggat tattttcctg
     1681 gcgcgcctct gtcacccttg cgttatgtgt gactgtttgc gcctctgcga tgggatgcgt
     1741 cgcgttcacg gatgacatgc ggctccccga gcagacgacg actgctccgg cctcgtcgca
     1801 caactccatc tcggcaacct cggacaccca caacgaggag ccacactccg tgacgtcgct
     1861 cgagatccca gggcaatcgt ttcttgccac gagcacggat gatccgactt ggcgttccct
     1921 cgtggggaac gcgctggcaa gcccatgctc aagtcatgtc gcgaggagag tggctcccgc
     1981 cgaggcggac acatgcggta aggaaacgct catggcacgg acggcgtttc tcgtcagaga
     2041 tgatatggcg ctgcgccctc cgagcaggca cctctggtta taccatccga gactggtccc
     2101 gcttaaggac ggtgaatctt ctgttcgcat gccgcccggc gagcttgcct acgaggacga
     2161 tgcggggcat ctcagattta gaaatcccta tacgggcctc cgcgaccaac cgcttttgca
     2221 tcgcgacacc gccagggccg cttcttcgga ccccaacatc tacgatatcg gcccgcctcc
     2281 gaagcacacc atcaagacct tcgcaggcct cacggcaatg agtcttatgg gcataggtgt
     2341 ctatgccctc ggaccgtcgt cgttcaccgg agccagcaaa gaaggtgaag atgtgtgggg
     2401 ggaggcgcgg gaccacttca aggaagcttg gaccaaacca ccggtattcg atagcgacag
     2461 ggcggtcgtc aattacttgg gccacccctt ctttggagcg cagttttatc tgtcccagag
     2521 gaattatggt gagagcccct tatacagctt tctgttctca accttcacat ccacctgctt
     2581 cgaatatttc atcgaaagct ggtcggagcg cccgagcatc acggacctca tcgtcacacc
     2641 cattgtcggg tcgatccttg gcgaactcgt ctaccaggcg actcgacaca tgcgcaagga
     2701 cggcttcacc caagcggaaa agat
//
LOCUS       DASTQY010000341         2730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1771250, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000341 DASTQY010000000
VERSION     DASTQY010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771250"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFR82_11230"
     CDS             complement(<1..223)
                     /locus_tag="VFR82_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEU4502212.1"
                     /translation="MPGQVVVTGLGIISPIGIGVSEFWKAALEGHSGVSTITSFDPFP
                     MDGYRSKVAGQIHGFAPERYLDSVQAERVD"
     gene            complement(330..1421)
                     /gene="lptG"
                     /locus_tag="VFR82_11235"
     CDS             complement(330..1421)
                     /gene="lptG"
                     /locus_tag="VFR82_11235"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEU4502213.1"
                     /translation="MPILFRYLLREYAKIFLMCFAGLMTIYLVIDFFEKVRRFLSFDA
                     TWLDVLSYFALKIPGVSYQIVPLAVLMATLLTLGLLSRNNEITAIRSCGISLPWITSP
                     FLTFAVIMSLVLLLFSSTVIPLATNKAEEIRVIRIEKKPPSAVLTLSQPWTRVDADSL
                     LHVSSLAPGGATLGGIQLFHFDPAFHLIDMTEAQEAHYAGNTWTLHRGFQRRFNRDGS
                     VTLVEFDRKPIHLAMIPSDFSSGLAGDSEMMTFQDIRESRGRLYQNGSQFTRLLTDYY
                     GRIAFPLVTIIMVLVGIALSLRRSGTRGGSMAIGIGQALLVGFSYWAAHSIAIAFGRG
                     GALPPMVAGWLTNVLFTSYGLYLMLKLKY"
     gene            complement(1428..2516)
                     /gene="lptF"
                     /locus_tag="VFR82_11240"
     CDS             complement(1428..2516)
                     /gene="lptF"
                     /locus_tag="VFR82_11240"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEU4502214.1"
                     /translation="MTLRTLLDRYIFTELLYPFSLSLGMLCFIMLTRELLRLVELLVT
                     KGVGFWAVLNVFAHLLPSFLVLTLPIAGIIASITAFGRLSLDKELVAMRAAGLSLLRL
                     ARPVMLFALLVCGMTLWLAQWGQPWSSINLKKVALSLLRDQLVLALERGSFTEPIPGM
                     TIYISDKDEQSSGGIFISDGRIASEPRVIVAQDYRVLIDSASDQVALRLKDGVIHTKP
                     DEIDEYQLASFSSYDLKLSLNQSGYASTEERPSYDQLVARLNNSQWRDPWALRRLTEY
                     YKDMAFPTASLILCMLGLPVGIVSKRSGRIGGFAVGVLVIIAYYVLNVACEFLVTATA
                     IPPFAGAWLPNSVFLVITLVSFYRMSKQ"
BASE COUNT          628 a          752 c          821 g          529 t
ORIGIN      
        1 ggtccacgcg ctcggcttgg acgctgtcga ggtaccgttc gggggcgaag ccgtgaatct
       61 ggccggcgac ctttgagcgg tacccgtcca tgggaaaggg gtcgaaactg gtgatggtgg
      121 agacgccgga atgaccctca agtgcggctt tccaaaattc gctcaccccg atcccgatcg
      181 ggctgatgat acccaatccg gtcacgacga cctgtcccgg cattctgtaa tccctcttgt
      241 gcttcgcgtc tttacttacc ggaagcgacg acgtgcgtca accgagtcgc gttaagtcga
      301 tgcctcggca gccgtgagtt cattgccctt caatacttga gtttcaacat gagatacagg
      361 ccatagcttg taaacaacac gttcgtcaac cagccggcga ccatcggtgg aagcgcgcct
      421 ccacgtccga atgcaatggc aattgagtga gcggcccaat aggagaaccc gaccagcaac
      481 gcttgtccga tgccgatcgc catgctgcct ccgcgggtgc cgcttcggcg aagactcagc
      541 gcgatgccaa ccaagaccat gatgatcgta accaacggga acgcgatacg cccgtaatag
      601 tcggtcagca accgggtaaa ttgcgatccg ttctgataga ggcgtcctcg ggattcacgg
      661 atgtcctgaa acgtcatcat ttccgaatcg ccggcaagtc cgctgctgaa gtctgaggga
      721 atcatcgcaa ggtgaatagg tttccggtcg aattccacga gcgtcacgga accgtcgcga
      781 ttgaagcggc gctgaaaccc gcggtgtaac gtccaggtgt tgccggcata atgagcctcc
      841 tgcgcttccg tcatatcgat gagatgaaac gctggatcaa aatggaagag ttggatgccg
      901 cctagagttg ctccgccggg agcaagactg ctgacgtgca gcaggctatc cgcatcgact
      961 cgtgtccatg gctgggagag cgtcagcacg gcagaaggcg gtttcttttc aatgcggatc
     1021 acacgaattt cttcggcctt gttcgtcgcg agcgggatca cggtggaact gaacagcaac
     1081 agcaccaagg acatgatcac ggcgaatgtc aggaagggcg atgtaatcca gggaaggctg
     1141 attccgcagc tgcggatggc ggtaatctca ttgttgcgcg agagcaaccc gagcgtcagc
     1201 aacgtggcca tcaggacggc gagtggaacg atctgatagg acacgccggg aattttgagc
     1261 gcgaagtagc tcagcacatc cagccacgtc gcatcgaagc tcaagaagcg gcggactttt
     1321 tcaaagaaat cgatgaccag atagatggtc atcaggcccg caaaacacat caggaaaatc
     1381 ttcgcgtatt cgcgcagcag gtatcgaaac aggatgggca tacgtggtta ttgtttgctc
     1441 atgcgataga agctcacgag cgtaatgacc aagaagacgc tgttcgggag ccaggccccg
     1501 gcgaatggcg ggatggccgt cgccgtcacc agaaactcac aggccacatt cagcacgtag
     1561 taggcgatga tgacgagcac acccacggcg aacccgccga tgcggcccga ccgttttgag
     1621 acgattccga cgggaagacc gagcatgcac aggatgagcg aagccgttgg gaaggccatg
     1681 tccttatagt actccgtgag gcgtcggagg gcccatgggt cgcgccactg tgagttgttc
     1741 aaccgggcta ccaactggtc gtacgaaggc cgttcctccg tcgacgcata tccgctctgg
     1801 ttgagagaga gtttgaggtc gtaactggaa aaggaggcca gttggtattc atcgatttca
     1861 tccggcttgg tatggatcac gccgtccttc agccgtaagg caacttggtc gctggcgctg
     1921 tcgatcagca cacggtagtc ctgagcgaca atgacgcgag gctctgaggc gattcgtccg
     1981 tccgaaatga agatgccgcc ggaagactgt tcgtctttgt ccgagatgta aatcgtcatg
     2041 cccgggatcg gttcggtaaa cgatccgcgc tccagtgcca atacgagttg atcccgcagc
     2101 agactcagcg ccaccttttt caggttgatc gaactccagg gctgtcccca ttgcgcgagc
     2161 cagagagtca tcccgcacac cagcagggcg aacagcatca caggacgtgc cagccgcagt
     2221 aggctcaagc cggcggcgcg catggccacc agctccttgt ccagcgacag ccggccaaag
     2281 gcggtgatgg acgcgatgat gccggcaatc gggagggtca gcacgaggaa cgacggaagc
     2341 aggtgggcaa aaacgttgag gactgcccag aaccccacgc ctttggtgac gaggagctcg
     2401 accagccgaa gcagctcccg ggtcagcatg atgaaacaca gcatgccgag gctgaggctg
     2461 aaggggtaga gcaattcggt gaaaatatag cggtctagca gcgtacgaag cgtcatgagc
     2521 ggcggctagc tcccgatatt gcgacactat aggggactgg taccggcttg tccacacggc
     2581 gggcaggacc cttttgcggt gagagcggct tgacaagatt agaatgccta tgttacatgc
     2641 tgcgcgcgcg atttgtaacc aagatgggtc tcgtgagtac gtcttttctt ctgcacagag
     2701 cccgcccatc cgtccccggt tctcatattc
//
LOCUS       DASTQY010000342         3031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1681514, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000342 DASTQY010000000
VERSION     DASTQY010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681514"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2300
                     /locus_tag="VFR82_11245"
     CDS             <1..2300
                     /locus_tag="VFR82_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEU4502215.1"
                     /translation="GIVLLLRGGNAREVVEAVKEKIDEIHEQKLLPAGLRIVPFYDRL
                     ELIVAALHTVYKALLEGIVLVILVLFLFLGNVRSALIVTATLVVAPLATFIVMDQVGL
                     TANLMSLGGLVIAIGMMVDGSVVVVENVYRHLSEQTHVSPTSRTAIVLKATKEVAQPV
                     VFGILIIILVFLPILALQGMEGKMFKPLAYTIMIALLVSLLLSLTLSPALCSLALTTA
                     TEEDTWLLRQAKRWYSPALTWALARRRVVLISAVTMLGLSLMLFPFLGGEFIPILNEA
                     AITPQTIRLPSISLEKSIEIELEMQRAVMEFPEVRMVVSKIGRSELGNDPQEPNASDP
                     VVSLKPMDEWTTAKTKPELDDAVRRRIERVPGANFLLSQPIQQRVDELLSGVRSEATV
                     KIIGEDLEQLRKTAEQVQAIMAGISGVKDVRVEQLFGQAYLSIDIDRGKIARYGINVA
                     HIREIISTAIGAEAATRVYEGQKRFDLILRYPKKYRDSVETISNILLRASTGALIPLG
                     ELAKVELREGPALISREGLQRRIYVGFNTLGRDIESIVGEAQAKIAKHVQLPTGYRLI
                     WGGSFENMQRAMARLKIIVPITIGLIFLLLFASFNSARHAALIIMNLPFALIGGIVAL
                     WVTGEYLSVPASVGFINLFGVAVLNGIVLVSYINQLRAEGLSDYDAIVRGCLLRLRPV
                     LMTALVAMLGLIPLAFSHGIGSEVQRPLAVVVIGGLVSSTLLTLIVLPTLYGVFAGSP
                     QTLMADSRARQRLSLPADRPAPSTH"
     gene            2528..>3031
                     /locus_tag="VFR82_11250"
     CDS             2528..>3031
                     /locus_tag="VFR82_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502216.1"
                     /translation="MPTSRAPLPSTFWVRLSLSALAGICLITQLQCGGGGDSNGGGSS
                     SPGVTGDVKAIARENTLPGTSAWRIQNPETGGEIAAYPDRESYPGGAEVRISVSANPP
                     GNFRWQVFRMGSYGRVGGRLYAEGGPHPAPRQPDPTFDPVTGLVVAGWPPTFSFPTRR
                     SDGTPWLT"
BASE COUNT          573 a          878 c          907 g          673 t
ORIGIN      
        1 caggcatcgt cttgctgttg cgcgggggca atgcccgcga agttgtggaa gccgtcaagg
       61 agaagatcga tgagattcac gagcagaagc tccttcccgc cggccttcgg atcgtgccct
      121 tctatgaccg tcttgaattg atcgtcgcgg cgcttcacac ggtgtacaag gcactcctcg
      181 aaggcatcgt gctcgtcatt cttgtgttgt ttctcttcct cggcaacgtc aggagcgcgt
      241 tgatcgtgac ggcgacactg gtggtggcgc cgctggcgac gttcatcgtc atggatcagg
      301 tgggcctgac ggccaatctc atgtcgctgg gagggcttgt gattgccatc ggcatgatgg
      361 tcgacgggtc ggtcgtcgtg gtagaaaatg tctatcgcca cttgagcgaa cagacgcatg
      421 tttcgcccac cagccgcact gccatcgtgc tgaaggcgac gaaagaggtc gcgcaacccg
      481 tcgttttcgg cattctcatc attattctcg tatttctgcc gatcctggcg ctgcagggca
      541 tggaagggaa gatgttcaag ccgctcgcct acacgattat gatcgcgctg ctggtgtcgc
      601 tcctgctgtc gcttacgctg tcgccggcgc tctgttcgtt ggcgctcacc actgcgacgg
      661 aggaagacac gtggttgttg cggcaggcca agcgatggta ctcaccggcg ctgacctggg
      721 cgctagcccg tcgccgcgtg gtcctgatct cggcggtgac gatgctgggc ctcagtctca
      781 tgctctttcc gtttctcggc ggcgaattca ttccgattct caacgaggcg gcgattacac
      841 cccagacaat ccggctgccc agtatttcct tggagaaatc gattgaaatc gagctcgaga
      901 tgcagcgcgc cgtcatggaa ttccctgaag tccggatggt ggtctccaag atcggtcgct
      961 cggaactggg caacgatccg caggagccca atgccagcga tccggtcgtt tcgcttaagc
     1021 cgatggacga atggacgacg gccaagacca agccggaact ggacgatgcg gtacgccgac
     1081 gcattgagcg tgtaccggga gcaaacttcc tgctcagtca acccatccag cagcgggtgg
     1141 acgaactgct gtccggtgta cggtcagagg cgacggtcaa gatcatcgga gaagacctcg
     1201 aacaacttcg gaagacggcc gaacaggttc aggccatcat ggcgggaatc agcggcgtca
     1261 aagacgtccg cgtcgagcaa ttgttcggac aggcctacct ctcaatcgac atcgaccggg
     1321 ggaagatcgc ccgatacggt atcaacgtcg cgcacatcag ggaaatcatc tctacggcca
     1381 tcggcgcgga ggccgcgaca cgtgtttatg aaggccagaa acgattcgac ctcattctcc
     1441 gctatcccaa gaagtatcgg gacagcgtgg agacgattag caacatcctg ctgagagcgt
     1501 ccacgggagc cctgattccc ctgggcgagc tggccaaggt cgaactgcgc gagggcccgg
     1561 cgttgatcag cagggaaggg cttcagcgca gaatctatgt cggcttcaat accttggggc
     1621 gcgatatcga aagtatcgtc ggagaggccc aggccaaaat tgccaaacat gtgcaactgc
     1681 cgacaggcta tcgtttgatc tggggcggat cgttcgaaaa tatgcagcga gccatggccc
     1741 gtctgaagat cattgtgccg attacgatcg gactcatttt tttgctgctg ttcgcttcgt
     1801 ttaattccgc tcgtcatgcg gcattgataa tcatgaatct cccgttcgct ttaatcgggg
     1861 gaatcgtggc gctctgggtc accggtgaat acttaagcgt gccggcgtct gtcggattca
     1921 tcaatctctt cggagtcgct gtgctcaatg gcatcgtcct cgtgtcctac atcaaccagc
     1981 tgcgagccga agggttgtcg gactacgacg cgatcgtgcg cggatgcctg cttcgactcc
     2041 ggcctgtgct gatgaccgct ctcgtggcaa tgttgggcct gatcccgctg gccttttcac
     2101 acggaatcgg ctcggaagtt caacgtccgc tggccgtcgt cgtgattggc gggctcgtca
     2161 gctcgacatt gttgacgctc atcgtgctac ccaccctcta tggagtattt gcaggctctc
     2221 ctcagacctt gatggcagac tctcgcgctc gccagcggct tagccttcca gccgatcggc
     2281 cggcgccttc cacacactga caagttgtcc caaaagtctc ctggtccgga caccttgtcc
     2341 ccaccttcct cgggcgacga tgggctaccc aggccctagg gaaggccatg gcttctcctc
     2401 aggggtggtg cgcttcttgc tgacggcttg gcgtgcaatg agtgcagaca tcgctcgctg
     2461 ataatggtcg ggcgagcggc tcttcgcacg ccatgagttc gttttggata ttgaggaggc
     2521 tttcgtcatg ccgacatccc gagcgccgct cccttcgact ttttgggtcc gtctcagctt
     2581 atctgctctt gccggcatct gcttgatcac gcagcttcag tgcggcggag gcggagacag
     2641 taatggaggt ggctcgagtt ctcccggcgt gaccggtgat gtcaaagcca tcgcacggga
     2701 aaataccctg ccgggaacct cggcttggcg cattcaaaac ccggagaccg ggggtgaaat
     2761 tgccgcctat ccggaccgtg aaagctatcc aggtggtgct gaggtccgta tttcagtctc
     2821 tgcgaatccc cccggaaatt ttagatggca agtgttcagg atggggagtt acggaagggt
     2881 ggggggtcgg ctctacgcgg aaggtggacc ccatcccgca ccgcgccagc cagaccccac
     2941 gtttgacccc gtgactggtc tcgtggtcgc gggatggcct cccacattct cctttccaac
     3001 aaggcgcagc gacggtacgc cgtggctcac t
//
LOCUS       DASTQY010000343         4926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_399303, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000343 DASTQY010000000
VERSION     DASTQY010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399303"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VFR82_11255"
     CDS             complement(<1..371)
                     /locus_tag="VFR82_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502217.1"
                     /translation="MAGLMSAEDVFEKTQEAAVAATAPDEKALQIEYPALRDKIRAAL
                     ADRKVALCHINKFLPEGYEEQGRFNVLLLTAGNVFFDMVIGDSYFRYDVVSVAQLDKV
                     QVIDAMWDNKEKRREEPFLSLR"
     gene            complement(424..690)
                     /locus_tag="VFR82_11260"
     CDS             complement(424..690)
                     /locus_tag="VFR82_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502218.1"
                     /translation="MFEKECRMLLQLMSVAGALMVLTAYGLNQGGVWRELHAGYLMLN
                     IVGSLMLGVVAIADQRAGFILLEFAWAGLGLLGVARVIRSRRQP"
     gene            complement(859..2256)
                     /locus_tag="VFR82_11265"
     CDS             complement(859..2256)
                     /locus_tag="VFR82_11265"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEU4502219.1"
                     /translation="MSPSREGLPPDSPFIDQAELEQPSLPDGTAVGDITSHSAVLWLK
                     TAGSKHVQVEWASVEAWKTMSAMASVRSAAARTPVLVTSVDTDFTLSILIDGLNPGSR
                     YRYYIVMVGPENSSKRHVAAEGEFGTLPDPATSAAVTFAWSGDLECRQGPEGYPIFDT
                     IRKQRPDFFIFLGDTIYADHLCPSPPNEPGANFVAKTLSEYRARHHYQRGAAALRRML
                     QTVPVYVMWDDHEVKNNFSGPFETQMPAGRQALRDYWPIQIDATDSHRLYRRVRYGSD
                     LEVFLLDTRQYRSRNADPDGPAKTMLGAAQLTWLLDGITRSTATWKVIVTSVPLSVLK
                     GGGLTVPGYDGWAGGPEGNGFERERRLIVDAILEKKLKNVVFLAGDLHWVQANVYDPN
                     EDGVADFHEFAVGPLSARPGWLTAPSATLHPTTLINEAGYENFGLVRVTKEAFGLSVL
                     DEAGVTRFFYRIVAR"
     gene            2451..2690
                     /locus_tag="VFR82_11270"
     CDS             2451..2690
                     /locus_tag="VFR82_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502220.1"
                     /translation="MNDLQCMIQGCQAKVYSPAGTQYVCKEHFLSFLTWRRRKGAQMF
                     VHYAGLSMDQRDPIVAEWSKTVSIIEAPSITASNP"
     gene            complement(2767..3159)
                     /locus_tag="VFR82_11275"
     CDS             complement(2767..3159)
                     /locus_tag="VFR82_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU4502221.1"
                     /translation="MLERRIEEVTKANQRFYEAFESLDIAKMDEVWAHLDYVTCVHPG
                     WTLRSDWPAVRDSWVLIFNNTFSMKFELTESMVQVAGDMAWVICIENITSQQSDEPQQ
                     ARVLATNLFERIGDEWLMIHHHGSAVMG"
     gene            complement(3307..4212)
                     /locus_tag="VFR82_11280"
     CDS             complement(3307..4212)
                     /locus_tag="VFR82_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4502222.1"
                     /translation="MAIQPAGFGYGAIVVAAVLWGGSIVAQKLALSGFSAVEASVLRD
                     IGGLIILMTTWWIQDGSLRGLTRQDYRTLALLGLGVLGNHLLILIGLKYVSGAVAGVI
                     IGALPVVTALLSAVLSQDVPLRLVWAGSVLSFFGVALVSVAGFNAAGEQPLLGGALVF
                     LGVASWALYSIGSRTIMERVPALTVNWTTLLAATVLQIPLLWTDQKMLKAGWASVALP
                     DWVALVYLVVFATAVAQQAWLFGVNRIGPSRASVLGNLTPVSAVALSAVVLKEPVGIV
                     EIIGILLILAGVWVVHRQTSSLPSS"
     gene            4363..>4926
                     /locus_tag="VFR82_11285"
     CDS             4363..>4926
                     /locus_tag="VFR82_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU4502223.1"
                     /translation="MWPNFTEFGAVLCDLDGVITQTARLHAAAWKRLFDDYLQEVAAR
                     TGAPFKSFDLGEDYRLYVDGKPRHEGVRDFLASRGVSLPSGTPSDGPDHETLYGLGQR
                     KDKHFKVALRETGVIPYPNTVKFLHLAHAMGLKMAVVSSSHHCAEIIETVGLSSLFDV
                     RVDGHEIDRLRLAGKPAPDSFLEAARRL"
BASE COUNT         1099 a         1487 c         1345 g          995 t
ORIGIN      
        1 cgcaggctga gaaaaggttc ctctctccgt ttctccttgt tgtcccacat ggcatcgatc
       61 acctgcacct tgtccagttg agcgaccgag accacgtcat agcgaaaata ggaatcgccg
      121 atgaccatgt cgaaaaacac attgcccgcc gtgaggagca ggacgttgaa ccgcccctgc
      181 tcttcgtaac cctccggcag aaatttgttg atatggcaga gcgccacctt gcgatcggca
      241 agggccgctc gaattttgtc gcgtaaggcc ggatactcga tctgcaaggc tttttcatcc
      301 ggagccgtcg cggcgactgc cgcctcctgc gtcttttcga atacgtcttc tgccgacatc
      361 aagccagcca taacgtcctc tctttctcta aggaaaaata tgcttactca cgcaccccta
      421 cggctagggc tgccgtcttg atcggatgac cctcgcgact ccgagtaggc cgaggccggc
      481 ccacgcgaac tccaacaaaa taaatccggc ccgttggtca gcaatggcca ccacacccag
      541 catcagagaa ccgacgatat tgagcatcag ataaccggca tgcagttcac gccagactcc
      601 cccctggttg agcccatagg ccgtcaagac catcaacgcc cccgcaacgg acatcagttg
      661 taagagcatt cggcattcct tctcaaacat ggtggcgctc cgtccataac gcgccccaac
      721 gacagcggtg ccagagcagc ggtgcaatgc gcgtggaacc atccagagca agccgggcgg
      781 gcgttttgca tcttcctaca aagccggtac ggtaaccggt ggacaagcag taatgcaagt
      841 ggcgagggga cggccgcgct agcgagctac gatgcggtag aaaaatcgtg tgactccggc
      901 ctcatcaagc acggacaggc caaatgcctc tttcgtcact cggaccaaac cgaagttctc
      961 ataccctgcc tcattgatga gcgtcgttgg atgcagcgtc gcactcggcg ccgtcaacca
     1021 gcccggtcgt gcggacaatg ggccgaccgc aaattcgtgg aagtccgcca cgccgtcttc
     1081 attggggtca tagacgttgg cctggaccca gtggaggtcg ccggcgagaa acacgacatt
     1141 tttcagtttc ttctcaagaa tggcatccac aatcagtcgg cgttctcgct cgaatccgtt
     1201 gccctctggc cccccagccc aaccgtcata acccggaacg gtcagcccgc ctcccttcag
     1261 gacggacaat ggcacactcg tcacgatgac tttccaggtc gcggtcgaac gggttatccc
     1321 atccagcagc cacgtaagtt gtgcagcgcc caacatcgtt ttggccggac cgtctggatc
     1381 tgcatttcga ctgcgatact gccgggtgtc cagaagaaac acctcgaggt cagagccgta
     1441 ccggacgcga cgatataagc ggtgtgaatc ggttgcgtca atctggatcg gccagtaatc
     1501 cctcagggcc tgccggccgg ccggcatttg ggtttcgaac ggtcctgaga aattattttt
     1561 tacctcatga tcgtcccaca tcacatagac agggaccgtc tggagcatcc ggcggagtgc
     1621 ggccgcgccg cgttggtaat ggtgcctggc acgatactcg gacaaggtct tggcgacgaa
     1681 gttcgcgccc ggctcattgg gtggagaggg acatagatgg tccgcataga tcgtgtcacc
     1741 gagaaagata aagaaatccg gcctctgttt gcggatcgtg tcgaaaatcg gatagccctc
     1801 agggccttgg cggcattcta gatcgccgct ccaggcgaag gtgacagcag ctgaggtcgc
     1861 aggatcgggc agcgtgccga actcgccctc agcagcgaca tgcctcttgg acgaattctc
     1921 cgggccaacc atcacaatgt aatagcggta ccgagaacct ggattgaggc cgtcgatgag
     1981 gatggataaa gtaaaatccg tgtcgacgct ggtcaccaac actggcgttc tggcagcggc
     2041 tgatcgcacc gacgccatcg ccgacatcgt tttccaggct tcgacggagg cccattccac
     2101 ttggacatgc tttgagccgg ctgttttcag ccaaagcacg gcagagtggc tcgtgatatc
     2161 tcccactgcc gtgccgtctg gcaagctcgg ctgctcgagc tcagcttggt caataaacgg
     2221 gctgtcgggc ggcaaccctt cgcgcgaggg tgacacgcat ccctggggga gcgcgaggac
     2281 agcgcacagt agaagcctta ccgtcacgtc cacggagtac tcaccgggag gcaaaagata
     2341 cgcgactgtc cgtctgaaca aagcaaggct ttgactgacc gtgtcctctc agggttgaaa
     2401 atcggatcca cggtcctgca tactgcttcc cgacaacgag gagccgcgac atgaacgatc
     2461 tccagtgcat gatccaggga tgccaggcga aagtctattc gccggcgggc acgcaatatg
     2521 tgtgcaagga acattttctg agcttcctca cctggcgtcg ccgcaaagga gcgcagatgt
     2581 tcgtccatta tgccggtctt agcatggacc agcgcgaccc catcgtcgcg gaatggtcga
     2641 aaaccgtatc aatcatcgaa gcgccttcca taactgcttc caacccttaa tcagcgcctt
     2701 catcgcctca ccgtctggac ggctctgaac gcacgtgagc ctcgttattt ttattcgtcg
     2761 ccttgttcac cccatgacgg cactgccgtg atggtggatc atcaaccact cgtcgccgat
     2821 gcgctcgaag aggttagtgg ccagcacccg ggcttgctgc ggttcgtctg attgctgact
     2881 tgtaatgttc tcgatgcaga tgacccaggc catatctccc gccacctgaa ccatcgactc
     2941 tgtcagttcg aatttcattg aaaatgtgtt gttgaagatc aagacccagg agtcgcgcac
     3001 agccggccag tctgaacgga gcgtccaacc gggatgcacg cacgtcacgt agtccagatg
     3061 cgcccatacc tcatccatct tcgcaatatc caggctctca aaggcttcgt aaaacctctg
     3121 atttgccttt gtaacttctt cgattcgtcg ttccagcatg cgcacactca acagcaaacg
     3181 atcgcttcac cggtaccggc cattgcatct cgagcacgca cctgagcgtt cagttcccag
     3241 acacgtcagt caagcgcgct atcatactgg aatccaccgc ggcagcgcga gagggtttgc
     3301 cgccggctac gaggaaggca ggctcgatgt ctgccgatgc acgacccaca cgcccgcgag
     3361 tatcaacaga ataccgatga tttcaacgat gccgacgggc tccttaagaa cgaccgcaga
     3421 gagcgcgact gcggaaacgg gcgtgagatt gccgagcacg gaggcccgtg atgggccgat
     3481 gcggttgacg ccgaacagcc aggcctgctg cgcgacggcg gtggcaaaca ccactagata
     3541 gacgagtgcg acccaatccg ggagcgccac cgatgcccac ccggccttca acatcttctg
     3601 gtctgtccag agtagcggaa tttgcagtac tgttgctgcc agaagcgtcg tccaattgac
     3661 ggtcagtgct ggaactcgtt ccatgatcgt tcgactgccg atgctgtaca gcgcccaact
     3721 cgcgacgccc agaaacacca gcgctccacc cagcaaaggc tgttcacccg cagcattgaa
     3781 tcctgccacg gaaaccaacg cgactccaaa gaacgacagg acactgcctg cccacacaag
     3841 ccgcaatgga acgtcttgac tcaagaccgc tgacaataag gctgtcacca ccggcagggc
     3901 cccgatgatt acacccgcca ccgccccgct gacatacttg aggccgataa ggattaggag
     3961 atggttgcca agcaccccca ggccgaggag agcaagtgtt cgataatctt gcctcgtcag
     4021 tccgcgcagt gatccatcct gaatccacca ggtggtcatt aggattatga gtccgccgat
     4081 atcccgaagc acggaggctt cgacggctga aaagccgctc agcgcaagct tttgcgcgac
     4141 gatggatccc ccccacaaga cagccgcgac gacgatcgct ccatagccga acccggcagg
     4201 ttgtatggcc atgccgtggg gatacaatcc ttccggccaa cggtcaagca tattcttgag
     4261 cgacatgcct ggatgcgctc gggaatttgc caggtgtttt tcggaagcca attccgtttg
     4321 atggaattga acaccggctc acatcggatc aaggcttcta acatgtggcc caacttcact
     4381 gagtttggcg cggtcttatg cgatctggat ggcgtgatca cccagacggc acgcttgcac
     4441 gctgctgcct ggaagcgcct gtttgacgac tatttacagg aagtggccgc ccgaacagga
     4501 gctccgttca agtcgttcga tctgggagag gactaccgac tgtatgtgga cgggaaacct
     4561 cgacatgagg gcgtacggga cttcttggcg tcccgtggtg tgagtcttcc gtcaggtacg
     4621 cccagtgacg ggccggacca tgaaacgctc tatggactgg ggcagagaaa ggacaaacat
     4681 ttcaaagtcg ccctgcgtga gaccggagtc atcccctatc ccaacacggt caaattcctt
     4741 catcttgcac atgcgatggg attgaaaatg gccgtcgtgt cttcgagcca tcattgcgcc
     4801 gaaatcattg aaaccgtcgg cttgagctcc ctgtttgatg tccgagtgga tggtcatgag
     4861 attgaccgtc ttcgtctggc aggaaaaccc gctccggatt ccttcctgga agcggcgaga
     4921 aggctg
//
LOCUS       DASTQY010000344         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1589036, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000344 DASTQY010000000
VERSION     DASTQY010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589036"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..598
                     /locus_tag="VFR82_11290"
     CDS             86..598
                     /locus_tag="VFR82_11290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4502224.1"
                     /translation="MSLPNQLTIRAANLDDVGSIITFSAAMARETENRRLDEARLREG
                     TLSLLNTPSYGFFVVAELRDGDQRKLIGQLMITYEWSDWRNGVFWWIQSVYVDPAWRR
                     QGVFRRMHDVIVGRAKEDPRACGIRLYVEQDNRTAQTVYERVGLSPSAYAVYEQDFVL
                     ARASRAKDSF"
     gene            611..997
                     /locus_tag="VFR82_11295"
     CDS             611..997
                     /locus_tag="VFR82_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502225.1"
                     /translation="MSICRPFVLLAALTLLAQGCAFSRGTLGDDIKTDAVGTIKKGVT
                     TRTEVLALLGAPDRILPLNGRDLYQYYRYDAKAGSLLLILVNFSRLSVKSDDLFVIVN
                     QAGVVEDVISSKRTESLEFRFWPFGE"
     gene            999..1424
                     /locus_tag="VFR82_11300"
     CDS             999..1424
                     /locus_tag="VFR82_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502226.1"
                     /translation="MLPRHPKIIAFFLLIIIGLLSSGCNVMRITLNTPITPKDVAFIV
                     PGQTTLPEVVERLGSPDSLNNTSTGIVSIYRFLDMKYARVNLGWIARFWSPVDPDLIF
                     SRTGFGTDALQVFYDSNGVVIEHSFLRHLKELPFHPYPF"
     gene            1711..2847
                     /locus_tag="VFR82_11305"
     CDS             1711..2847
                     /locus_tag="VFR82_11305"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU4502227.1"
                     /translation="MSCIAAHAGDSDEPRARARDLGIVIGSHASGPLNAITDVAGVKV
                     GHTTLISGDGPLKPGHGPVRTGVTVVIPREDVWHQKVPAGSFVLNGTGEMTGLAWVSE
                     AGFLEYPIALTNTLNVPRVANGVMTWMIGKYPEIGITDDTLTPVVAECDDGRLNDIQG
                     RHVSEADVMNALNGATGGPVKEGTVGAGTGMVSYGFKGGIGTASRALPEADGGYTVGV
                     LVNANHGRKPELVVSGIPVGRLYDGSDAGPPSRPGQSEGSIIIVVATDAPLDGRQLNR
                     LAKRAALGLARTGSTARHGSGDFIIAFSTANVIPHYPQERTYALTHLADTHLNPLITI
                     TVEATEEAVINALTAATTVIGRDNYKVEAISLSRLRTILGTSSK"
BASE COUNT          651 a          804 c          790 g          614 t
ORIGIN      
        1 acgccatgaa aatggaattc tctgccggct tctataagcc ggcagagggc tggctcggag
       61 cccctaaaat cacgacatag ccctcatgtc cctgccgaac caactgacca ttcgagcagc
      121 caatctggat gacgtgggat ccatcatcac gttcagcgca gccatggcgc gcgaaacgga
      181 gaacagacgg cttgatgagg cgaggctcag ggaaggaacc ctgtcgctgc tcaacacacc
      241 atcgtacgga ttcttcgtgg tcgcagaact ccgcgacggg gatcagcgta agttgatcgg
      301 ccaactcatg atcacgtacg agtggagcga ttggcgcaac ggtgtctttt ggtggattca
      361 aagcgtctat gtcgacccgg cgtggcggcg ccaaggtgta ttccgacgga tgcacgatgt
      421 catcgtcgga cgagcgaagg aagatccaag agcctgcggc atccgtctct acgtggagca
      481 ggacaaccgc acggcgcaaa ccgtctatga gagggtagga ctttctccat cagcttatgc
      541 ggtatatgaa caggatttcg tcctggctcg ggccagtcga gcgaaagatt cattctaaga
      601 aggagattct atgtctattt gtcgaccatt cgtgttgctt gcagcgctaa ccctactcgc
      661 tcaaggatgc gccttcagcc gaggcaccct cggtgacgat atcaagaccg acgcagtcgg
      721 tacgatcaag aagggcgtta cgacgagaac agaagtgctc gcgctcttgg gagcccccga
      781 tcggattctt cccctcaatg gacgggacct gtaccagtat taccgttatg acgccaaggc
      841 tggaagcttg ttgttgattt tggtcaattt ttcgcgtctg tccgtcaaga gcgatgatct
      901 gttcgtcatc gtcaatcaag ccggcgttgt ggaagacgtg atttcgtcta agcgaactga
      961 aagcctggag ttccgcttct ggccattcgg agaataagat gctgccgcgg caccccaaaa
     1021 tcatcgcttt cttcttgctc attatcatcg gcctgctgtc ttcaggctgc aacgtgatgc
     1081 gcattacctt gaataccccg atcaccccca aagacgtggc cttcattgtt ccagggcaaa
     1141 caacattacc tgaagtcgtc gaaagactcg gatcgcccga ttcattgaac aatacgagca
     1201 ccggtatcgt ttcgatctat cgcttccttg acatgaagta cgcgcgcgtc aatctcggct
     1261 ggatagctcg attttggtcc ccggtagacc ccgacttgat cttctcccgt accgggttcg
     1321 gcaccgacgc cctgcaagtc ttctatgatt caaatggggt ggtcattgag catagttttc
     1381 tgagacatct gaaagaactc ccatttcatc cttatccctt ttaagccgtt gccagttcaa
     1441 gctgcaggcg cgaattgccg cttccggccg gcctcgctat aatctggcca atatggatga
     1501 acgctggcac ccgtctcgcc cttttctccc tgcgcgtcac ggcattaaca cgtcatgaac
     1561 gagacaagcg acctgccttt tcccgggctc gaaccggcca cacctgagct aattcagaaa
     1621 gcacacactg tgcagcctat aatttgcctc aggactgatc gtcggctggt tggacagcag
     1681 gctcttctat tggtgctgtg ctgcctgctt atgtcctgca tcgcggccca tgcgggtgat
     1741 tcggacgaac cccgggcgcg ggcgcgagat cttggcatcg tgatcgggtc gcacgcgagt
     1801 ggtcccctca acgcaattac ggacgtcgcc ggcgttaaag tgggacatac gacgttgata
     1861 tcgggcgacg gaccattaaa gccaggccac ggccccgttc ggacaggcgt gacggtcgtc
     1921 attccgagag aagatgtgtg gcatcaaaaa gtccctgccg gctccttcgt actcaacggg
     1981 acgggggaga tgacgggact ggcctgggtg agtgaagcag gatttctgga atacccgatc
     2041 gccctcacta acacgttaaa cgtacccaga gtggccaacg gcgtgatgac atggatgatc
     2101 ggaaaatatc cggaaatcgg aatcaccgac gacacgttga ctccggtcgt ggcagaatgt
     2161 gatgacggcc gcctgaacga catacaaggc cgtcatgttt cggaagcaga cgtcatgaac
     2221 gccctcaacg gtgccaccgg cgggccggtg aaagaaggca ccgtcggagc ggggaccggc
     2281 atggtgtcat acggattcaa gggaggaatc ggcaccgcct ctcgggcgct gccggaggcc
     2341 gatggcggtt atacggtcgg cgtcctagtc aacgcgaacc acggtcggaa accggagctg
     2401 gtggtctcag ggattccggt cggccggctc tacgatgggt ctgatgcagg tccgcctagc
     2461 cgtccaggcc aaagcgaagg ctcaatcatc attgtcgtgg caacggacgc gccacttgat
     2521 ggaagacaac tgaatagact cgccaagcga gccgcattag gattagcaag gactggctca
     2581 acagcccgcc acggcagcgg tgacttcatt attgcgtttt caaccgctaa cgtcatcccc
     2641 cattatccac aagaacgaac ctatgccctc acgcaccttg cagatacgca tctcaatcct
     2701 ctgattacca taaccgtcga agcgacggaa gaagctgtca tcaacgcatt gaccgcggct
     2761 acgacggtca ttggacgcga caactataaa gtggaggcca tttcattgag ccgcttgcgc
     2821 acgattctcg gaaccagcag caaatagaag cgttgctta
//
LOCUS       DASTQY010000345         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_875618, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000345 DASTQY010000000
VERSION     DASTQY010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875618"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(381..758)
                     /locus_tag="VFR82_11310"
     CDS             complement(381..758)
                     /locus_tag="VFR82_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502228.1"
                     /translation="MAKILVIDDDAQDRGLIGAVLEERGYEVILAENGRTGLMACHRQ
                     HIDGVVLDLRMPEIDGRSVLQQLRTLHPSLPVVVFSGHGTEEVEQELLNRGATAFIQK
                     AFSLDKLGLVLQEVLPPPNKSDD"
     gene            1015..1320
                     /locus_tag="VFR82_11315"
     CDS             1015..1320
                     /locus_tag="VFR82_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502229.1"
                     /translation="MKMLTIVCREQFEEEVLAVFSNLKISGYTVVHGVGGSGETGEVS
                     VMHTSYDHNKLILVALDDDRINLLVKGVKQLLARLVEERFGDPVPLKAFVQPCEPVV"
     gene            1390..1566
                     /locus_tag="VFR82_11320"
     CDS             1390..1566
                     /locus_tag="VFR82_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502230.1"
                     /translation="MKNVEMTVEGTLLTIKVDLSKEFGPSSSGKTIIIASTEGNVTIP
                     NREEKVGLNVYRKK"
     gene            1743..2144
                     /locus_tag="VFR82_11325"
     CDS             1743..2144
                     /locus_tag="VFR82_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502231.1"
                     /translation="MKRRSAIIAIISVTCLLGLLSFSNPGEAGERTVGKIDPAALKNQ
                     VIGQEATLSLDSLTASKTFRDLPSISGNYTVKGKRIMPYIGAGFGHGYTSDVDRSLNG
                     SPSSQTDPGLRSLFGQGLTPSEFQMGLRIPF"
     gene            2208..2687
                     /locus_tag="VFR82_11330"
     CDS             2208..2687
                     /locus_tag="VFR82_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502232.1"
                     /translation="MRQRHRWWFILVLIVLLAINGCHTSRQSSTPLLTDNGRFMDMWG
                     IYTHCSRTEDLEAMRVDAQRLSLAGNMTDSAGDPIAPENEEPASVGPSVRLSADPSAM
                     AAACALRAGQVAQERGRRSFAREMFQMIVTNFPQPRYRYYVGQAREGLERLDAETSG"
BASE COUNT          661 a          759 c          845 g          632 t
ORIGIN      
        1 ggtcagtcct tgtgctcgcg aaaggcggca catcagaaaa agaagggcgt gagggctggt
       61 tgatctcctg tgctcgccga acgcgcgcac cagatccatc gaattcttag caagtcggtg
      121 ctcgttcaag cgcgcagtag gagatcaaac cagccccgcc ccggagagag tacgagcgag
      181 cttggaaggc catttgtatg ggcggtgctc gtccaagggc gcagtaggac cagcatggct
      241 accactccat caaggggagg cacaagccag cttggaagga gcatcgaaac gcgcgcacgt
      301 ttggactgat caaggctgtt ccaagcgagc ttggtagaaa catttggtgc aggttaggca
      361 gggattgtaa ccgctgatgt tcagtcgtca gacttattgg gaggggggag aacctcctgc
      421 aataccaatc ctaatttatc caacgaaaag gccttttgga taaaggccgt ggcgccgcgg
      481 ttcagcagct cttgctccac ttcttctgtg ccgtgtccac taaacacgac gactggtagg
      541 gaggggtgca gggtacgcaa ctgctgcagg acgctgcgac cgtctatttc aggcatgcgg
      601 aggtccaaca cgaccccatc aatatgttgc cggtgacagg ccatcaaccc cgttcgacca
      661 ttctctgcca aaatgacttc ataccctctt tcctccaaga cggcacctat gaggccacgg
      721 tcttgggcat cgtcatcaat gacgagaatc ttggccatat gagctctcat atcctcgttt
      781 gtcataccat tcgtccttgg acagaatagc agccttcgag tgtttcatct agaaaagttg
      841 gagttgaggg agcgcccacc atgctctgag catccgcctg gccggatggt ttggatacga
      901 cctggcgaag gcagtggcct caaggcgtca cggcggaatt gtgcggacga tacggcgtga
      961 cttcatccgt acgcgggagg cacaataagg cttcaacgat caggaggaat gctcgtgaag
     1021 atgcttacca ttgtatgtcg agagcagttc gaagaagagg tgctcgccgt gttcagcaat
     1081 ttgaagattt cagggtacac ggtggtgcac ggggtgggcg gcagcggcga aaccggcgag
     1141 gtctcggtca tgcacacatc gtatgatcac aacaagctga tccttgtggc gctcgacgat
     1201 gatcggatca acctcctcgt caaaggcgtg aagcagttgc tggcccggct ggtcgaagaa
     1261 cgtttcgggg atccggttcc gcttaaggct ttcgttcagc cttgcgaacc agtcgtgtaa
     1321 cgagcaaagc gtgcgctctt gtttgttagg ttaggcgttc agactacgaa cgaggaggaa
     1381 gataccatta tgaagaacgt tgagatgacc gtcgagggaa ccctgctgac gatcaaggtg
     1441 gatctctcca aagagttcgg gccctcttcg tctggaaaga ccatcatcat cgcctcgacg
     1501 gagggaaatg tgacgattcc gaatcgggag gaaaaggttg ggctcaacgt ctaccgaaag
     1561 aagtagggga ggcatcgtcc tcatcatccg atgacaccgt ttcagccgct gaaccgcccc
     1621 gctgaatact cctctcgagc gccgcttgtt gcccttcctt gaattaatgg gtggggtcgc
     1681 ggatcttgac tcctgagcat tgtgttttat atgttgaaaa ccatggaaag ggaccctggc
     1741 agatgaagcg ccgtagtgcg atcattgcga tcatatcggt gacgtgtttg ctgggcctct
     1801 tgtcattctc caatccggga gaggcaggtg agaggactgt cggcaaaatc gacccggccg
     1861 ctttgaagaa ccaggtgatt ggccaggaag ccaccctatc tctggactca ttgacggcct
     1921 cgaaaacgtt ccgggacctt ccctccatca gcggaaacta cacggtgaag gggaagcgga
     1981 tcatgcccta tatcggtgca ggttttggtc acggctatac gtcagatgtt gaccggtcgt
     2041 tgaatggcag cccttccagc caaactgatc ctggtcttcg aagcttgttt gggcagggtc
     2101 tcaccccgag tgaattccag atgggtctcc gcatcccgtt ctgatcagcc caaccccctg
     2161 acgacgttct gctgagaaca acaggaaaga aggaaagagt gcgtcttatg cgtcaaaggc
     2221 atcgctggtg gttcatcctc gtactgatcg tgctgctggc aatcaacgga tgccatacga
     2281 gcaggcaatc ctcaacacca ttgcttactg acaatggccg ctttatggat atgtggggca
     2341 tctacaccca ctgctcccga actgaagacc ttgaggcgat gcgtgtcgat gcacaacgat
     2401 tgagcctcgc cgggaacatg acggactccg ccggagatcc catagcccct gagaatgagg
     2461 aacccgcgtc cgtgggaccg agcgtccgcc tctcggcgga tccatcggcg atggctgcgg
     2521 cctgtgccct tcgtgccggt caggtcgcgc aggaaagggg acgtcggtcc tttgcacgag
     2581 agatgtttca gatgatcgtc acaaactttc cgcaaccccg ctatcgctat tatgtgggtc
     2641 aagcccgaga gggtctcgag cgactggacg ctgagacatc cggatagcga gaaaagatgt
     2701 cgggaccatt tctaccggct catactcacc aacattcacg ggatcaagaa aagcggacag
     2761 gctacttagt tggctgggcc gactcgcaaa tacagtcatt catgctgttg ctgcgggcag
     2821 tcaggaacct gctctgcgcg aggaattttc ggctgagcgg aggttgagac gaaggggaca
     2881 gactacgttt ctgtctt
//
LOCUS       DASTQY010000346         6521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1527905, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000346 DASTQY010000000
VERSION     DASTQY010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527905"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFR82_11335"
     CDS             complement(<1..183)
                     /locus_tag="VFR82_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502233.1"
                     /translation="MPVLQINGIGLHYEESGSLSQPTVVFAHSLMWGANTFTELLTEI
                     ARHARLVTFDLHGHGLS"
     gene            complement(396..899)
                     /locus_tag="VFR82_11340"
     CDS             complement(396..899)
                     /locus_tag="VFR82_11340"
                     /inference="COORDINATES: protein motif:HMM:NF022606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2938 domain-containing protein"
                     /protein_id="HEU4502234.1"
                     /translation="MNLEANVVVGAIAVGLGATLVMDLWNLFLKRAFRILSLNYCLLG
                     RWLRHMPEGRFRHANITAVPPKPFECTVGWIAHYTIGVVLAVAFVLASGEWLVRPTLL
                     PALLYGIGTVVFPFFLMQPSLGLGIAASRAPNPTQARLKSLATHTVFGIGLYLCALGV
                     SYLLLFT"
     gene            complement(998..1432)
                     /locus_tag="VFR82_11345"
     CDS             complement(998..1432)
                     /locus_tag="VFR82_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502235.1"
                     /translation="MTEYKSDLLFGALTGNGRILVIDDEPCVRNVVRMVLEKAGYDVL
                     EADNGETAIEAINTGENRLVLDAVICDIRMPKINGIQAIDYFKKQFPHVPIIVLTAYP
                     EAEMAVSFMRSGVVDYLVKPVEAQKLTAAVERAMERRQLTWA"
     gene            1804..2814
                     /locus_tag="VFR82_11350"
     CDS             1804..2814
                     /locus_tag="VFR82_11350"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006850.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEU4502236.1"
                     /translation="MKEELLRLARRMPKAELHLHIEGSLEPEMAFRLAKRNGVRLQYP
                     DVNSLRSAYAFNNLQEFLDIYYTGMSVLMKEEDFYDLTWAYLKRVHQDNVVHVEIFFD
                     AQGHLERGIPLDVQINGIRHALQDGKQQLGISSKLILSFLRHLSEEHAFKTLEMAQPF
                     LDLLDGFGLDSSEIGNPPEKFARVFARCRELGFKTTAHAGEEGPADYVSQALDLLQVD
                     RIDHGNHSLDDAALVQRLTTEGKTLTVCPLSNLKLCVVKAMDQHPLLTMLKGGLKATV
                     NSDDPAYFGGYVNDNYRALIEHLPVTREDLYQLARNSFNGAWVGQEEKTKHLNDLNRV
                     FA"
     gene            complement(2928..3386)
                     /locus_tag="VFR82_11355"
     CDS             complement(2928..3386)
                     /locus_tag="VFR82_11355"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU4502237.1"
                     /translation="MADIIHRVGIKAPISKVYAALSTVEGIAGWWTKHTSGSSKIGET
                     IGVRFHSADGKEIGSMNMEVKALDPNKKVHWRFTSGPEEWVGTDVVFTLSQEGDYTIV
                     LFGHKNWRDANEFTSHCSMKWATFLLSLRELVETGKGKPSPNDVKIDDWN"
     gene            3714..4037
                     /locus_tag="VFR82_11360"
     CDS             3714..4037
                     /locus_tag="VFR82_11360"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4502238.1"
                     /translation="MGFKRTSSRVEVGKPGRLHRGSFSAPCQVMNVSETGVRIQSRLF
                     VKNGDVLQLSIELDGGRQLGCEIQALNVGSRQFGAKILSISPDDRERLSHILDDHMQN
                     RFLLG"
     gene            4550..5083
                     /locus_tag="VFR82_11365"
     CDS             4550..5083
                     /locus_tag="VFR82_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502239.1"
                     /translation="MNNRRLNVLASIGLAVGGVLGMTGTFAPSAALRGLAWGIDGAAL
                     VMAGGILTVVFYRTGQDLVAAGFLVFAIGEGTILSSAAMDLDASVPSFAAGSILWAMA
                     LVLISLPPVFPFPVRTLGLTTASMFASTALQIFSGEHLTPLSSPLPFFAYPFFVATFV
                     GWIWTLLKGHGPLLPER"
     gene            complement(5097..5549)
                     /locus_tag="VFR82_11370"
     CDS             complement(5097..5549)
                     /locus_tag="VFR82_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502240.1"
                     /translation="MTRFIGCIVVLMLFNIAEVQAQGYIDGLMNRATESAKRKAQDRV
                     NQNIDQSIDKAIRKTEDTVKCVATDQECLRRAKEEGKQVEMVGKPVANDTMKCLITDS
                     GCMKQAKALHKKVEIVEEDQLDTLRCSTTDTDCLARAKTKGKKVEIID"
BASE COUNT         1459 a         1809 c         1668 g         1585 t
ORIGIN      
        1 acttaggcca tggccatgaa gatcgaacgt cacgagacgg gcatgtctcg caatttccgt
       61 cagcagctcg gtgaaggtgt ttgcgcccca catgagcgaa tgggcgaaca cgaccgtcgg
      121 ttggctcagg ctccccgact cttcataatg aagcccgatc ccgttgattt gaagaaccgg
      181 catacgctct ctcccgatag tggcaagcaa cgacttttcc agtgctcgcg cacgacacta
      241 ctgggaaaga gtctcttgat tcaagatatc agagacaaag gggggaaatg gggacaattc
      301 cggagaagag aagggaacag cttggggcat tcgctgcggg ttgacgtgcg tcgcagatgc
      361 accctccaga tggcttcgtc gcgggcaaca caatctcagg tgaacagcaa aagatagctt
      421 actccgagtg cacagagata cagtccaatt ccgaatacgg tatgtgtcgc aagacttttc
      481 agcctagctt gagtgggatt aggtgctcgc gaggctgcga tgccgaggcc aagcgacggc
      541 tgcatgagga aaaatgggaa cacgacggtg ccgatgccat agagcagcgc aggcagcaag
      601 gtcggtcgga caagccattc ccctgatgcc aggacgaacg caaccgccaa cacgactcca
      661 attgtatagt gggcgatcca gccgaccgtg cactcaaagg gtttcggggg cacggcggta
      721 atattcgcat gccgaaacct gccttcgggc atatggcgaa gccaacgccc gaggaggcag
      781 tagttgagcg agagaatcct gaacgcacgc ttcaggaata gattccacag gtccatgaca
      841 agagtcgccc caaggccaac ggcaatggct ccaacaacaa cgtttgcctc caggttcatg
      901 ccctaacccg atagggaagt cgctcgctca gtataattcg gcggtcggac acaagaccgc
      961 agtttctgga actatttcgc gctacgcagc caatgcctca tgcccaggtc agctgtcgtc
     1021 gttccatcgc tctttccacc gcagccgtga gcttctgcgc ttccaccggc ttgaccagat
     1081 aatccaccac cccactccgc atgaacgaga cggccatttc agcctccgga taggccgtga
     1141 gaacaatgat cgggacatgg gggaattgtt tcttaaaata atcgatggcc tggataccgt
     1201 tgatcttcgg catgcggatg tcgcagatca ccgcatccaa tactagtctg ttttctcctg
     1261 tattaatcgc ttcaatggct gtctctccat tgtccgcttc taacacgtcg tacccggcct
     1321 tttccagcac catccggact acgtttctga cacaaggttc atcatctatc acgagaattc
     1381 gtccgttccc ggttaacgcc ccgaacaata gatccgattt gtactcagtc atggtgttct
     1441 cccttcttta catccgtttt agtaccgctg ctcctaccat gacgctgagc aacgtccgtg
     1501 ccacttgaaa acactgtctc tagctaagat agttggtaga ggctcttact caaaggcatc
     1561 caaactgatg ctctgtggat gtaatccgat ccacattgct acaaaaggaa gacggcatca
     1621 gccattctcg agtaagccga gtgaggtcaa attcattgga tcaaacggcg catgatggcc
     1681 ggctatgagg ttacgattac cgatgcctag cacatgaggg tcggacgtaa agtggtatgc
     1741 ttctctcagt gggcttgtca ttcacatttc cggaatatcc ccctttatta tgaaggtata
     1801 tccatgaagg aagaattatt gcgactggcc cgccggatgc ccaaggccga gttgcatttg
     1861 catatcgaag gcagtcttga accggaaatg gccttccggc tcgccaaacg caatggcgtt
     1921 cggctgcaat atcccgacgt gaactccctg cgttcggctt acgctttcaa taatctgcag
     1981 gaattcctgg atatctatta caccggcatg tcagtcttga tgaaggaaga ggatttttac
     2041 gatctcacat gggcatacct gaagcgggtg catcaggaca acgtggtgca tgtggagatc
     2101 ttctttgatg cgcagggaca tctggagcgc ggcattccgc tggacgtgca gatcaacggc
     2161 atacgccatg cgctgcagga tggcaagcaa cagctaggta tcagctccaa gctgattttg
     2221 tcattcctac gccacctgtc ggaagagcat gcctttaaga ccttagagat ggcccaaccc
     2281 tttctggatc tgctggatgg cttcggcctg gattcctcgg aaataggcaa cccgccggaa
     2341 aagtttgcac gagtgttcgc gagatgcagg gaactcggtt tcaaaaccac cgcccatgcc
     2401 ggtgaggaag gcccggcgga ctatgtcagt caggcgctgg atttgctgca agtggatcgc
     2461 attgatcacg gcaaccactc gctggatgat gcggccctgg tgcaacgcct gaccaccgag
     2521 ggcaaaacgc tcaccgtctg tcctctgtcc aacctcaagc tctgtgtggt gaaggccatg
     2581 gatcagcacc cgctactgac tatgctcaaa gggggcctga aggcgaccgt caactcagat
     2641 gaccctgcct atttcggcgg gtatgtgaac gacaactacc gcgcgctcat tgaacacctc
     2701 ccagtcaccc gcgaagacct ctaccagctg gcacgcaatt cctttaacgg tgcctgggtc
     2761 ggtcaagaag agaaaacgaa gcacctgaat gatctcaacc gggtgtttgc atagccgcac
     2821 tgccttggca tgtcacggtg cgtacggcac ctccttctcc ggtgaaggca gaatgcccca
     2881 gttgcgacct gcattttgtc gcctcgatca attgatcgca cggcgctcta attccaatca
     2941 tcaattttta catcgttcgg cgagggcttc cctttaccgg tttccacaag ttctctgaga
     3001 ctcaacagaa atgttgccca tttcatgctg caatgagagg tgaactcgtt tgcgtcgcgc
     3061 cagttcttat gaccgaaaag cacgatcgtg taatcaccct cctgagaaag ggtgaatacc
     3121 acatcagtgc ccacccactc ttctggtccc gatgtaaagc gccaatgcac tttcttgttg
     3181 ggatcaagcg ctttgacttc catgttcatg ctgccgattt cttttccatc tgcagaatgg
     3241 aagcgaacgc caatcgtctc tccaattttt gaggagccgg aagtgtgttt tgtccaccag
     3301 ccggcgatgc cttcgactgt cgataacgcc gcatacacct tagagatcgg tgccttaatt
     3361 ccaactcgat gaatgatgtc tgccatgtgt cctccaatgg gttttggttt ctcaggtttg
     3421 cgttcgaact cagtggcgcg gctgagccaa ccactgaagc ggctctatct ccttgtcgaa
     3481 caacgcagac gaattcgaca cgggaactaa cttggcgata taacgatagt tccttgagag
     3541 acattgaccc ttcacgccca ataccgtcat ggtccaaaat ttgtcgtcga gcattcccat
     3601 cgtgcaccac aaggaagaac cattccaccc tcgcgaacga cgagcgaaca ttgtgaatgt
     3661 gctttgccgt tgcataccca actgctatcg ttcccgtggc aggagacaag tcgatgggtt
     3721 tcaaacggac atcgtcccga gtcgaggtgg gcaaaccagg cagattgcat cgtggctctt
     3781 tctcagcgcc ctgccaagtg atgaatgtca gcgagactgg agtgaggata caaagccgct
     3841 tattcgtgaa aaatggggat gtgcttcagc tttcgatcga actcgatggc ggccgtcaac
     3901 taggctgcga gatacaggca ctcaacgtcg gctcccgtca gtttggcgcg aagattctct
     3961 caatcagtcc cgacgaccga gagcggttgt cccatatcct cgacgaccac atgcagaacc
     4021 gctttttact cggatagaaa ccgagattct gccgccactt cccgtcatct cgggagtgtc
     4081 gacccgcttt tcttagcacc tccgaacttc aagccaattt gctctcagct gaggatggtc
     4141 acaacaagaa atggcacgag gttcaaaagt actatgccaa ttttatacag ggacataccc
     4201 gcgtagtgca gagcgtcgaa ttgttcgttg gacagatgga accatctacc gtgtaagtga
     4261 cgtggacggt catgtgcgag cacgaagact agaaactaca caagcaggat cgtgtaattc
     4321 atgacggtgc tccagaggag aaagttgcgg gtgagttcag cgctcatcgt tccccctccc
     4381 tcggcattgc agaaattgtg acaagcgatt cttgccccag ttcagacaat tgtcaacgct
     4441 acacactggc ttcctctctg atgtgatgga tacgcctttc ccctttccgc tacactgctg
     4501 cgacgctatc tactgagtgc accagtgccg ctatcgaggc gacgcaagca tgaataatcg
     4561 acggttgaac gtgctcgctt cgatcggtct ggctgtggga ggagtgctcg gcatgacggg
     4621 gacgtttgcc ccctctgcgg ctctccgcgg tctcgcgtgg ggaattgacg gcgctgctct
     4681 ggtgatggcc ggcgggattt tgactgtcgt gttctaccga acgggacaag atctcgtggc
     4741 ggcaggtttt ctcgttttcg ccataggaga aggcaccatc ctgtcgagcg ccgccatgga
     4801 tctcgacgcc agcgttccat catttgcagc cggaagcatt ctctgggcga tggcccttgt
     4861 tctcataagt ttaccgcctg tctttccttt tccagtgcga acgctggggc tgaccacggc
     4921 gagcatgttc gcatcgacag cgctgcaaat cttctccggc gagcatctca cccccctctc
     4981 gtcccctttg ccattcttcg cctatccctt cttcgttgcc acattcgtcg gctggatctg
     5041 gacgcttttg aagggtcacg gcccactcct cccagagaga tgacttccgg ccctctctag
     5101 tcgatgatct cgaccttctt cccctttgtc tttgcacgtg caaggcaatc ggtatcggtc
     5161 gttgagcaac gtagcgtgtc tagttgatct tcctcaacta tctcgacctt cttgtgcagt
     5221 gccttcgcct gcttcataca tccgctatct gtgatgagac atttcatggt gtcattggcg
     5281 actggctttc caaccatctc gacctgttta ccctcttctt ttgcgcgtct gaggcactct
     5341 tggtccgttg cgacacattt aaccgtgtct tcggttttcc tgatagcctt atcaatggat
     5401 tggtcgatgt tctgattgac gcgatcctgc gctttgcgct tggcgctttc ggtcgctcga
     5461 ttcatcagac catcgatata cccctgagcc tgtacctccg caatattgaa caacatcaac
     5521 acgacaatgc acccgatgaa acgtgtcatg atcagtggcc ctcccttcaa agcatttgta
     5581 aataagaata gcagatcgga gaaccccgcc tgccaaggag aaggaaaaga cgtcaggaac
     5641 cagcgtcggg gcacgggctt tatacaagaa atgtcttagg tgtagcctag cccagtcgca
     5701 ggcccgcttg gagagcaccg gcgtaaagcc gtagcacttt cttccccaaa gagattagaa
     5761 tgccacgtct ttttgggcga acttaaacaa gaaatgggga caggctactt tcccgagacc
     5821 cttgccagcc gccctcgcgt tctcaatgtc gattccagcc caagtacagc agcgattccc
     5881 tgtccggcac tgttctgata cgaacggctg tcaccggctt acaagcgtag gatcgtgagc
     5941 ttctggtcaa agagcagcag gtcaatgacc cagcctattc ctgtatggat gcttattttc
     6001 gttggatgga tcctcgtcat agtggttctt tgcagctttc cttggtgggt agaatcgccg
     6061 caatgggatc gcgtgagatg gattccactg cttgatgtgc tccgatcacc gcgtcggctc
     6121 cttcgggata ctattgccaa cggtctcctc tacattcctt tgggctttgc ttatgcgaga
     6181 atgcgagcgg tgcctgggtt taatttgatt tccgaggcgg cgtttgtcgg gttattgctg
     6241 tccattacgt gtgagcttta ccaagtattt tcacccgtcc ggtttccttc gatgactgac
     6301 gtcctgatga acaccattgg tgcgtcagca ggagcttcca tcgcagccaa gtctttggcg
     6361 agactgagcg cacgaaaagg gaatgggctc ctttcctgat gcctttggtt cccgtcccgg
     6421 tttctgggca ttggaagaga cgcgcgacac aggttcttcc ccttcaagtg gccatcacac
     6481 caggatgtcg cctcttgaaa aatacatgaa agttcttgac a
//
LOCUS       DASTQY010000347         4032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1595311, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000347 DASTQY010000000
VERSION     DASTQY010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595311"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VFR82_11375"
     CDS             complement(<1..832)
                     /locus_tag="VFR82_11375"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEU4502241.1"
                     /translation="MPKTPKKSAVSLKTSRSSKTRTSSKTKTSNTPAKIYDQKIKRGE
                     GGELHQTASGATPVLTTAQGGPVQDDHNSLKISTRGPVLLEDFHFREKIFHFDHERIP
                     ERVVHARGYGAHGFFESYASLSALTKADLFQRPGERTPAFVRFSTVAGNKGSADLARD
                     VRGFAVKLYTKEGNWDLVGNNIPVFFIQDAMKFPDLIHAAKQEPDRGFPQAQTAHDNF
                     WDFISLSPESMHMVTWIMSDRTIPRSFRFMEGFGVHTFRLVNAEGKSTFVKFHWKPKL
                     G"
     gene            1144..1743
                     /locus_tag="VFR82_11380"
     CDS             1144..1743
                     /locus_tag="VFR82_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502242.1"
                     /translation="MNVKINELLQQMLETELGGVKVYETALTCARNQDLKDEWTKYLQ
                     QTHRHVEIVQALMQELGAGEVEMPSRYVVRSIGQSLVAAMNQAKTLGDPAGAEIVACE
                     CVVFAETKDHQNWQLLSRCAESAEGPAAAALLKACGEVEDEEDEHIYHSKGWCRELWL
                     QALGLEAVLPPPEEVRDVKSAAEAAKVEQSRKKAVAARH"
     gene            complement(1894..2799)
                     /locus_tag="VFR82_11385"
     CDS             complement(1894..2799)
                     /locus_tag="VFR82_11385"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU4502243.1"
                     /translation="MIDCLIVGGGPAGLTAAIYLARFRRRIMMVDAGYSRALLIPHTN
                     NFPGFPEGISGAELLERLRHQVSRYEIEIVKDSVEAIEIASDHIFATTSSMTIRARKV
                     LLATGVIDITPPASACRKGIADGAVRFCPICDAYEIQGQRVAVLGPAQKAIREALFLK
                     TYTRDLTILATDGASGLASEQRAVLKLAGIEFVEFSDIDLAVADGEVRAFLADQDYLR
                     FDIVYSALGTDVRSGLALPLGASCSDAGCIVVDAHQRTSIPGIYAAGDVVHELSQISV
                     AIGHAAIAATDIHNSLAEASGERAL"
     gene            complement(2899..3648)
                     /locus_tag="VFR82_11390"
     CDS             complement(2899..3648)
                     /locus_tag="VFR82_11390"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU4502244.1"
                     /translation="MLESSAALAHSIDCRAIIRRQTDVTTSPAIVASGWACYARSRRK
                     GRRQILSFILPGDSIGLAEGPFAPGLSEIVALTGMVLLDASNIQQALAESHCANLRMA
                     FYFERGLQEARLLDLVVQLGQSSLERTAHLLLELHHRMDRAGLVRNEWFHLPLAQHQL
                     GAALGLSHIQMHRTLQTLRSRGLLVLNGREATLPHVDELEQAAGYEVTPGARWEIENP
                     NVPASSDDQSSHQWSHDDSAVVEPDTQNERA"
BASE COUNT          848 a         1145 c         1228 g          811 t
ORIGIN      
        1 tgcccagctt gggtttccag tggaatttga cgaaggttga cttcccctct gcattgacca
       61 ggcggaaggt atggacacca aatccctcca taaagcggaa ggagcgagga atcgtccggt
      121 ctgacataat ccacgtcacc atgtgcatgc tttcagggct cagggatata aagtcccaga
      181 agttgtcgtg cgccgtctgc gcctgcggga agccgcggtc gggctcctgc ttcgcggcat
      241 ggatcaggtc ggggaacttc atcgcgtcct ggatgaagaa gaccgggatg ttgttgccga
      301 ccaggtccca gttgccctcc ttcgtgtaca gcttgaccgc gaagcctcgc acgtcgcgcg
      361 ccaggtcggc ggagcccttg ttgcccgcca cggtcgagaa gcgcacgaag gcgggcgtgc
      421 gctcgcccgg ccgctggaac aggtcggcct tggtcagcgc gctcagcgac gcgtagctct
      481 cgaagaagcc gtgcgcgccg tagccgcgcg catgcaccac gcgctcgggt atgcgctcat
      541 ggtcgaagtg gaagatcttt tcccggaaat ggaagtcctc aagcagcacc gggccgcgcg
      601 tactgatctt tagagaatta tggtcatcct gaaccgggcc gccctgggcc gtcgtcaaca
      661 ccggcgtagc gccgctggcc gtctggtgca gctcgccgcc ttcaccccgt tttatctttt
      721 gatcataaat tttcgctggg gtgttggagg ttttggtctt ggacgaagtt ctggtcttgg
      781 acgagcggga ggttttcaac gagacggctg acttcttcgg cgttttcggc atggcttact
      841 ccagcgaacg ggtgggggct ccgttcgatc gcgaacaacc aaacaggcga ggtatgaacc
      901 ttatccgtgc ttgcgcgttc ccctctgcgg gcgctgtgag cgaacaacac gcaaaataag
      961 cattctgggg gccattcagg caggtccggg taagtcctcg cggaagcaga gcctggtagc
     1021 cgtggcaacc gcgcccgccg acgcagcact acagcttgtt gtcaaaaaac acgggaggaa
     1081 cgtactggac ccaacgcagt tcgcgtgtgg tctagtattc acgcgaaccc gaaaggcagt
     1141 gccatgaatg taaagatcaa tgaactcctt cagcagatgc tggagacaga actaggcggc
     1201 gttaaagtct atgaaaccgc gctgacctgc gcgcggaacc aggatctcaa ggacgaatgg
     1261 accaaatacc tccagcagac tcaccggcat gtcgagattg tgcaggccct gatgcaggaa
     1321 ctgggcgctg gcgaagtcga gatgcctagc cggtatgtcg tgcgaagcat cggtcagtcg
     1381 cttgtcgccg ccatgaatca ggctaagaca ctgggcgacc cggctggcgc cgagattgtt
     1441 gcgtgcgagt gcgtcgtgtt cgcagagacg aaggaccatc agaactggca gctcctgtcc
     1501 cgatgtgccg agtcggctga gggtcccgct gcggctgccc ttttgaaagc ctgcggcgag
     1561 gtcgaagacg aggaagacga gcacatctac cacagcaagg gctggtgcag ggagctttgg
     1621 cttcaggctc tcggcctgga ggcggttctg ccgccacctg aagaagtacg ggatgtgaag
     1681 tccgcggccg aagcggccaa agtcgagcag tcgagaaaga aagccgtggc tgcgcggcat
     1741 tagcggcggt tggtctgccg acgcgccggc agcggcgagt ttagtgggga gggctgtccg
     1801 cccgtcgggc ggccctcctg ggaggaaatc gttcgtagtt tggttgcatc tacgatacga
     1861 agctacggcg cgattcctcg acaggaagat gaactataga gcccgttccc ctgaggcctc
     1921 cgcaaggctg ttgtgaatgt ccgttgcggc gatggcggcg tggccgatcg ccacggaaat
     1981 ctggctgagt tcgtgaacga catccccggc agcgtagatc cctggtatcg aggtacgctg
     2041 atgggcgtcc accacgatac agccggcatc ggaacatgaa gcaccgaggg gaagcgccaa
     2101 ccccgaacgc acgtccgttc caagcgcgga gtaaacgata tcaaaccgga ggtagtcctg
     2161 gtcggcgaga aaagcgcgaa cctcgccatc cgcaaccgcg agatcgatat ctgagaactc
     2221 cacgaactca atacctgcca gcttaagcac tgcccgttgt tcagatgcca ggcctgaggc
     2281 gccatctgtg gcaaggattg tcaaatcccg cgtataagtt ttgaggaaca gcgcctccct
     2341 gattgccttc tgagcgggcc caaggactgc gacccgctgt ccctgtattt cgtatgcgtc
     2401 gcagatcggg caaaatcgca ctgcgccgtc tgcgattccc ttccggcaag ctgatgccgg
     2461 cggagtgata tcgattacgc ccgtcgccag gaggactttg cgcgccctta ttgtcattga
     2521 gctggtcgtt gcgaagatat gatcgctcgc tatctcgatt gcctctacgc tgtctttaac
     2581 tatctcaatc tcatagcgcg atacttgatg gcgcagtcgt tccagcaact ccgcaccgga
     2641 gatgccctct ggaaaaccgg gaaagttatt ggtgtgggga atcaggagcg cgcggctata
     2701 gccggcatcc accatcatta ttcttcgacg aaaacgcgcg aggtagatcg ccgccgtcaa
     2761 acccgcgggg ccgccaccta cgatcaggca gtcaatcaca gaatactttc tcgaggcatg
     2821 aatctagttc tccagcacta caaagaaacg gcgaggctgg tgagatctcg ctgttgacga
     2881 cgacgaccgg tctctcgatc aggcgcgctc gttctgggtg tctggttcga ccacagcgga
     2941 gtcgtcatgg gaccattggt gactagactg gtcatctgac gaagcaggga cattagggtt
     3001 ttcaatctcc caacgagctc ctggtgtaac ttcgtatcca gcagcctgtt ccagctcgtc
     3061 cacgtggggg agggtggctt ctcgaccatt taggacaagc agtccgcgac tacggagggt
     3121 ttgaagggtg cgatgcatct gaatatggct cagtccaagc gctgctccta gctggtgctg
     3181 cgcaagcggt aaatgaaacc actcattcct gacaagccca gctctatcca tccgatgatg
     3241 cagttccagc agcagatgcg ctgtacgctc caaggatgat tgcccgagct gcacgacgag
     3301 gtccaacaac cgagcttcct gcaggccccg ctcaaaatag aaagccatcc ggaggtttgc
     3361 acaatgggac tcggcaagcg cttgctggat gttcgatgcg tccagcaaga ccatgccggt
     3421 cagggctacg atttccgata atcccggcgc gaacggtcct tctgcaagac caatgctgtc
     3481 ccctggcaag atgaagctga ggatctgcct gcggcccttg cgcctcgagc gcgcatagca
     3541 ggcccagccc gaagcgacga tagctgggga ggtggttacg tcggtctgcc ggcgtatgat
     3601 cgccctacaa tcaatagaat gcgcgagggc ggcactgctt tcgagcaagg cgcgctcatt
     3661 ctcatcaaat gatactatcg atcgatgacg gtgcaggaag ggggcaatgt actctagcct
     3721 ctcagtctgt ggtctcgaat cggccttaag catactctcg ggcacaggat cttcctctct
     3781 gtcgcgcctc ggccgccgac gaagtgggtg gcggcgaaga ggccatcgag gccataccga
     3841 atggtacttc gccgcctgcg gccgatgcca taagcgaacg aaactcggcg acgggccaag
     3901 tgagtaacga tcccttgtga tgagagcgcc ttggtctagc gcatatccta ggcgggccgg
     3961 tgaagaggga ttctacgatc cagaggcccg ttgctgaaga ctttaggctc cgtacgtttt
     4021 cggacacaac aa
//
LOCUS       DASTQY010000348         8983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1389647, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000348 DASTQY010000000
VERSION     DASTQY010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389647"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VFR82_11395"
     CDS             <1..590
                     /locus_tag="VFR82_11395"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU4502245.1"
                     /translation="KPAVHSLLGIANDPNTIVRDRVVRALSAIALPPELVTRGLTQAL
                     QDPEWTVRYAAISPFSFGGVLNPESLAALRPLVHDPNEMVARLAQSAIAAADHPIPVS
                     VHIFSLDQGSDRTLPLLQLTKLGPRAAEAVPKLASLLTAERPLERYLAACTLESVGPG
                     ALQAVPALQRTLDDPDPIVREAAAEALHAIEARRR"
     gene            587..1075
                     /locus_tag="VFR82_11400"
     CDS             587..1075
                     /locus_tag="VFR82_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502246.1"
                     /translation="MRSPATDSPQRFTMLTVILYWICILSGLAIPWIATIAVDVAKHD
                     QSLAGAVRQLSLHLFAPGYNLFIIALINAIPFLIFAVFLLFHLGLSPSDNRTLRGRRS
                     AGVFLTVIGLVGVSLWTHLTTLWQADAQAALAYLFLPFLLLVMMPICYAFGRALSTLV
                     FR"
     gene            1132..1935
                     /gene="thiD"
                     /locus_tag="VFR82_11405"
     CDS             1132..1935
                     /gene="thiD"
                     /locus_tag="VFR82_11405"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU4502247.1"
                     /translation="MSRSLKQVLTIAGSDSGGGAGIQADIKAMSANGVFAMSVITAIT
                     AQNTEEVTEVFDLPTSIIAAQIDAVFDDFDVAAVKTGMLSSASIVETVAKMLKPQEVV
                     NLVVDPVMISKSGHSLLKPDAIEAVKKQLFPLAILVTPNVHEAQQLSGIQITSLADAR
                     RAAKVIHGLGCKHVLIKGGHLLAERATDLLYDGRFFNVFKGEFIDTPHTHGTGCTYAS
                     AIAAQLARGKTITDAVAAAKTYVTEAIRHSLAIGHGAGPTNHFYFLPES"
     gene            1932..3269
                     /locus_tag="VFR82_11410"
     CDS             1932..3269
                     /locus_tag="VFR82_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502248.1"
                     /translation="MKRSLLSSQSAGVERRPNARLARPSLSCTPLTFLLAGFTWLSLS
                     FLLGVALLLGLVNGTPLPHWLKPVHVHGALLGGLLQLIIGGFLFSRADDHGNAYAASH
                     SSLFWAFNGATVGLLVSFWLGHMTTAGLAALVLIGIVMWISPRAWLRIGNVYRQPAGA
                     GWIARTALVALLLGLIAAALMAFRVMEGYHSYLRLFHIHFLVLGFLTVTFILVLHQIL
                     PPLIQAPLASHALARISLWFLPVGFATLLGGFVTSTLWLEIAVGGLLVAAMALSTYNF
                     LAGWIKSGAPEHAVSDQLLIGVCFLFLTTIAGLAMGANYLRTPPVMPIGSLHLVAYTH
                     LAFIGFMTQVVCGTLATGVPDILAATRVPNSRKREVYRAQLDGVMNRWRTVQLAGMSL
                     GTMSLAVLAALTWSVPLGSSYVQITVWIGAGLLILSLTLFAVKLTWAVSLRPS"
     gene            3465..4703
                     /locus_tag="VFR82_11415"
     CDS             3465..4703
                     /locus_tag="VFR82_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502249.1"
                     /translation="MNDELTQIIPDPWRSLFHFLTIPLSWIPDMQHQVMGFVLASSAG
                     WTAGMYYLLLLLPAMLWIGAVWCTHLTLYTLPFRSGRIELLRMLLLAWWDTAFAMWMY
                     WVGLGRFLVVVLGWLFTLGRLGLNLAAEAMKQLVLLPFKMTGQMTQSYFQPGVPWIAF
                     LLLIFWCVLEATIFTYTLMPTVTEVLAGITGSDASVMTGTILYLFLLLLIMGSFACIQ
                     VLADSVKNQEYKFLVQMLLVELFVMTFEVMFLYRELVDALSPWIAQQTGEQFRPGIVF
                     TLSLATFGWMGVRGMTWFLFGQYGTPPLLSFISRQPMQQATAALDVSVLPTQPPVWWR
                     APLDDFKREVGWLHEKSGELMEFLMLPVLHVVAALLNFAMILVMGKPVFGIPFKSLKD
                     VVETRELLPTVRWQPKKVAS"
     gene            4700..5476
                     /locus_tag="VFR82_11420"
     CDS             4700..5476
                     /locus_tag="VFR82_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502250.1"
                     /translation="MNRKPMRPFTAVVISMLCLVLGCSQEAAQPYKPHNTLFIGVDAS
                     GSFKNSGYYDNALSFLAHYIYGHLHHLGGLEKPQALFVASVGGKNLQEPKSFHPIHDF
                     ENKGIPAIEAELRKWFPPGDRVTDFNPFFAEVARITKERNLVLAPINVIIITDGVPDA
                     TTNGAIAGTQGLYEKIDLKPLEYLSRNVTLRLAYISPKVGEQWRRHVPRERVRLWTVD
                     GEVMKNWKEKLQPDRDLAQQDRFWKWLQDTVDYRVRSSKM"
     gene            5539..6291
                     /locus_tag="VFR82_11425"
     CDS             5539..6291
                     /locus_tag="VFR82_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU4502251.1"
                     /translation="MTFHDDRGHRISAVLSRPEQPTTAISVLCHGFLSSKNSTTNKTL
                     TRMLNEKAIATFRFDFFGQGESGGHFEDITPSLAVGQTLAAVTLVQAQGFVKVGLVGS
                     SFGGLVSILTAAQRRDIACLALKCPVVDFAEELRLEFGPDELARWQVTGTIPNLMGGP
                     ERIRLKYGLYEDSLQQIAYGPAAQIVAPTLIVQGACDELVPLHQSQRLLDALRGPKRL
                     DILPHADHQFTRGEDFRTMTTAITDWLVGYLV"
     gene            6331..6780
                     /locus_tag="VFR82_11430"
     CDS             6331..6780
                     /locus_tag="VFR82_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502252.1"
                     /translation="MITEGVQQTLTVLYPLYKNEVYQRREHMIRLTAFGAFGLIAMIF
                     ALLLSPVKARLSPAESVLLALGGLTWCALFCGLVLQQQHRHHLAKQVLIQLEQALGFY
                     EDGLFVDHQTLYPAKWKTAWLTDRTGLHYYASLCGLTGIFLFALILV"
     gene            complement(7137..7487)
                     /locus_tag="VFR82_11435"
     CDS             complement(7137..7487)
                     /locus_tag="VFR82_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502253.1"
                     /translation="MRCSPRPPVPWLPEEIEETVPVFARPPTAALVIGAAFVNGSVVE
                     ALEAVAGALGTEEPVDARPVAGIDAWLSLPALVVSVVAGSAVVEVVCDGTAGRLASFR
                     GSGRGAGVDGAAAV"
     gene            7654..7884
                     /locus_tag="VFR82_11440"
     CDS             7654..7884
                     /locus_tag="VFR82_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502254.1"
                     /translation="MSWTSNSEADLAGYKLYVGTAPGHYTYAGSPFVIGLMGSYTVSG
                     LPMGQTYYFAISAFDSSGNESGLSSEVSKSIY"
     gene            complement(7925..8731)
                     /locus_tag="VFR82_11445"
     CDS             complement(7925..8731)
                     /locus_tag="VFR82_11445"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU4502255.1"
                     /translation="MLTIPIVFLLLCDATVSLAQLDRLRKSKSLMTSPIPDTPMVEVP
                     AGPFVMGSDGTQALEDERPAHRVWLDGFSIDAEEVTTAQYAAFLAATKHAAPWQWETV
                     DLMQHADRPVIGVDWHDADAFCRWKGKRLPTEAEWEKAARGTDGRLYPWGNQVPTPNL
                     ANFALGARFSYNQVLLPVRSHEQGKSPYGLYHMAGNAYEWVQDWYGTNYYEISPDRDP
                     PGPIQGQFKVLRGGSWSDLPKYLLTYGRFKLLPDTKNSYTGFRCAMSEKK"
     gene            8721..>8983
                     /locus_tag="VFR82_11450"
     CDS             8721..>8983
                     /locus_tag="VFR82_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502256.1"
                     /translation="MVNTINTLTHRRRRAGTRLLAGKSAGWISDEVSRGNLPQPKKRL
                     RTQLGQGCAAARGGWAGEKGQGRVSARPGWVGENKIVSTIPSV"
BASE COUNT         1727 a         2779 c         2490 g         1987 t
ORIGIN      
        1 cgaaaccagc ggtccattca ttgcttggaa tcgcaaacga tcccaacact atcgtacgcg
       61 accgagtggt acgggccttg tcggcgatcg cgcttcctcc cgaactggtc acgcgaggac
      121 tcacgcaggc gctgcaggat ccggaatgga ccgtgcgcta tgctgccatt agccccttct
      181 cgtttggcgg agtgctgaat cctgagtcgc tcgccgcctt gcggccgctc gttcacgacc
      241 ctaacgagat ggtcgctcgc ttagctcaat cggccatcgc ggcggcggat catccaattc
      301 ctgtctcggt ccatattttc tcactggatc agggaagcga ccgaacgctc cccttattgc
      361 aattgacgaa attggggccg cgtgcggccg aagccgtccc caagctggca tcgctgttaa
      421 cggcagagcg gccgctcgag cgttatctgg cggcctgtac actcgaatcc gtcgggcccg
      481 gcgcactgca ggcggtgccc gcgctgcagc gaacgctgga tgacccggat ccgatcgtgc
      541 gtgaggcagc ggcggaagca ttgcatgcca tcgaggcccg gcggcgatga gatctccggc
      601 gacggactca ccacaacgct tcaccatgtt gaccgtgatc ctgtactgga tctgtatcct
      661 gtcgggcctg gccatcccct ggattgccac catcgccgtc gacgtagcaa agcacgacca
      721 gtcactcgcc ggagccgttc gtcaactgtc cctgcatctc tttgcccccg gatacaacct
      781 attcatcatt gcgctgatca acgcgatacc tttcctgata ttcgcagtgt tcttgctgtt
      841 tcatctgggc ctctctccta gtgacaaccg aacccttcgc ggacgtcgca gcgccggtgt
      901 gttcttgacc gtgatcggcc tggtcggcgt cagcctgtgg acgcatctga cgacgctctg
      961 gcaggccgat gcccaagccg cgctcgccta tctgttcctg ccctttctgt tgctggtcat
     1021 gatgcccatt tgctatgcat tcggacgcgc gctctccacg ttggtctttc gctgacgctg
     1081 gacagacttc tgtcgcaaag tatatatatg cccactgatt tccactcacc gatgagccgg
     1141 tcactcaaac aagtgttgac gattgccgga tcggattccg gcggcggcgc tgggatccag
     1201 gccgacatca aagccatgtc ggcaaacgga gtcttcgcga tgtcggtgat tacggcgatc
     1261 accgctcaaa acacggaaga agtgacggag gtgttcgact tacccacttc cattatcgcg
     1321 gcacaaatcg acgccgtctt tgacgatttc gatgtggctg ccgtcaaaac tggcatgctg
     1381 tcgtccgctt cgattgtcga gacggtcgcg aagatgctga aacctcaaga ggtggtcaat
     1441 ctcgtagtcg atccggtcat gatttccaaa agcgggcatt cgctcttgaa accggatgcg
     1501 atcgaggcgg taaaaaagca gttgttcccg ctcgcgattc tcgtcacgcc caatgtccat
     1561 gaggcgcaac aactctccgg cattcagatc acctcactgg ccgatgcccg ccgcgcggca
     1621 aaggtgatac acggactcgg atgcaagcat gtgctcatca agggcgggca cctccttgcc
     1681 gagcgggcga ccgatcttct ttatgatgga aggttcttca atgtcttcaa aggcgaattc
     1741 atcgatacgc ctcatacgca tggtaccggg tgcacctacg cctcggcgat tgcagcgcaa
     1801 ctggcccgtg gcaagacgat cacggacgca gtcgcggcag ccaagaccta tgtgaccgaa
     1861 gcgatccgcc acagtttggc gatcggccac ggtgccggtc ccaccaatca tttctacttt
     1921 ctaccggaat catgaaacga tcactcctgt cctctcagtc agcgggcgtc gaacgccgtc
     1981 cgaacgcgcg tcttgctcgc ccctcgctgt cctgtacgcc gctcacgttc ctgctcgccg
     2041 gattcacgtg gctgtcactg tcgttcctgc tgggggtcgc cctgctgttg gggttagtca
     2101 atggtacgcc actgcctcac tggctcaagc cggtgcacgt acacggcgcg ctgctcggcg
     2161 gattgctgca actgatcatc ggtggcttcc tcttctctcg tgccgacgac cacggtaacg
     2221 cctatgcggc atctcactcg tctttatttt gggctttcaa tggtgcgacg gtcggccttc
     2281 tcgtcagttt ttggctcggc cacatgacca cggcaggcct ggcagcactc gtgctcatcg
     2341 ggattgtcat gtggatttca ccacgagcct ggctccggat cggcaatgtc taccgccaac
     2401 cggccggtgc aggatggatc gctcgcacag ccttggtcgc cttgctgctc ggcctgatcg
     2461 ccgccgccct gatggccttc cgcgtcatgg agggctatca cagttatctc cggctttttc
     2521 atatccattt cctcgtgctc ggatttctga cggtcacgtt catcctggtg ctgcatcaga
     2581 ttctgcctcc tctaatccag gcaccccttg ccagccatgc actggcccgc atctccctct
     2641 ggtttctgcc ggtcggtttc gcgaccttgc tcggcggatt tgtcacgtca acactctggc
     2701 tggagatcgc cgtcggaggg ctcttggttg ccgccatggc cttgtccacg tacaatttcc
     2761 tcgccggctg gatcaaatct ggcgcaccag aacatgccgt gtccgaccaa cttttaatcg
     2821 gtgtgtgctt tctctttctc accaccatcg ctggtctggc gatgggggcc aactacctgc
     2881 gcactccgcc ggtcatgccg attggatcgc tccatttggt cgcctatact cacctcgcat
     2941 tcatcgggtt tatgacgcaa gtcgtctgcg gcaccctcgc gacaggcgtg ccggatatct
     3001 tggcggcgac ccgcgtgccg aacagcagga aacgtgaggt ctaccgcgcg caactggacg
     3061 gcgtcatgaa ccggtggaga acggtgcaac tcgccggaat gagtcttggg acgatgtcac
     3121 tggcggtcct tgctgcgctg acttggagcg ttccgcttgg ctcttcatac gtgcagatca
     3181 ctgtctggat cggcgccgga ttgctgatcc tgagtctgac cttgtttgcc gtgaagctga
     3241 cctgggccgt cagcctacga ccttcctgac ttttcacgtc aaagacctcg tatcggtcct
     3301 gctccgtacc tcagagcctg caccccctgg acatttctgg ccaagcgatc cgggcgtaac
     3361 tggccgtctt tcaaccagtt gcagcctcat ctccccagcc tggttcttgc tcctatccaa
     3421 ggagaatcag gccgcacctg gccgactcac acaacgaggg actcatgaat gacgaactga
     3481 cgcaaatcat tcccgatccg tggcggagcc tctttcactt tctgacgatc cccctgtcct
     3541 ggatccctga catgcagcac caggtcatgg gattcgtgct ggcttcctca gcaggttgga
     3601 cagccggaat gtactacctg ctgctcctct tgccggcgat gctgtggatc ggagctgtgt
     3661 ggtgtaccca cttgacgctg tatacactgc cgtttcgctc cggccggatc gagttgctga
     3721 gaatgctgtt actggcatgg tgggacaccg ccttcgccat gtggatgtat tgggtgggcc
     3781 tcggacgctt cctcgtcgtc gtgctggggt ggctgttcac gctggggcga ctggggctca
     3841 atctcgctgc agaggcgatg aagcaactgg tgctcctgcc cttcaaaatg acgggacaaa
     3901 tgacgcaaag ctactttcaa cccggtgtgc cctggatcgc cttccttctg ttgatcttct
     3961 ggtgtgtgtt ggaagccacg atcttcactt acacgcttat gccgaccgtc acagaagtcc
     4021 tggccggcat taccggctcg gatgcttcgg tcatgacggg aacgattctc tatctgttcc
     4081 tgttgttgtt gatcatgggg agcttcgcct gtatccaggt gctcgcggac tcggtcaaaa
     4141 accaggagta taaattcctc gttcagatgc tcctcgtcga gctctttgtc atgacgttcg
     4201 aagtgatgtt tctctatcgc gagctcgtcg atgcactctc accctggatc gcgcagcaga
     4261 ctggcgaaca gttccgcccc ggtatcgtct tcacactctc cctggcgacg tttggctgga
     4321 tgggtgttcg aggcatgacc tggtttcttt tcggccagta cggcacccct ccgctgctgt
     4381 ccttcatctc acgccaaccc atgcagcaag ccacggccgc gctggacgtg agcgtgttgc
     4441 cgacgcagcc gccggtgtgg tggcgcgctc cgctcgatga cttcaagcgg gaagtcggat
     4501 ggctgcacga gaaatccggt gaactcatgg agtttctcat gctgccggtt ctccacgtgg
     4561 tcgccgcctt gttgaacttc gccatgatcc ttgtcatggg taaaccggtc ttcggtatcc
     4621 cgttcaagag cctgaaagac gtcgtggaaa cgcgggagct tctccccact gtcagatggc
     4681 agccgaaaaa ggtggcctca tgaaccgcaa gccgatgcgg ccattcactg ccgtggtcat
     4741 cagcatgttg tgccttgtct tgggctgctc gcaggaggcg gctcaaccct ataaaccgca
     4801 caacacgctc ttcatcgggg ttgatgcgag cggttcgttc aagaactccg gctactatga
     4861 caatgccctg tctttccttg cccactatat ttatgggcat ctgcatcact taggcgggct
     4921 ggagaaaccg caagccctct tcgtcgccag cgtcggcggc aagaacctac aggagcccaa
     4981 gagcttccat cccattcacg atttcgaaaa caaaggcatt ccggcgatcg aggccgaact
     5041 ccgcaagtgg tttccgcccg gcgatcgagt gaccgacttc aacccgttct ttgcagaggt
     5101 agctcgcatc accaaggagc gcaatctcgt cctcgcgccg atcaacgtca ttatcattac
     5161 cgatggggtg ccggacgcca ccaccaacgg cgcgatcgcc gggacgcagg gcctgtacga
     5221 aaaaatcgat ctcaagccgc tcgaatactt gtcccgcaat gtgacgcttc gcctggcgta
     5281 tatcagtccc aaagtcggcg aacagtggcg caggcatgtt ccccgtgaac gggtgcgcct
     5341 ctggacggtt gacggagagg tcatgaaaaa ctggaaagaa aagctgcagc ccgaccgtga
     5401 tctcgcacaa caagatcgat tctggaaatg gctgcaagat acggtcgact accgcgtgcg
     5461 atcgagtaaa atgtaaatcc ggcttcctcg acgtccgccc gaagatgtct gctacaatgc
     5521 ccctgcatgg aagagtcgtt gacgtttcac gacgatcgag ggcatcgcat ctcggcggtc
     5581 cttagtcgtc ccgaacagcc taccaccgcc atcagcgtgc tctgccacgg ctttctctcg
     5641 agcaaaaaca gtacaacgaa caagaccctg acgaggatgt tgaacgagaa ggccattgcg
     5701 acttttcgat tcgatttttt cggtcagggc gaaagcggcg ggcattttga agacattacg
     5761 ccatcgctgg cggttggcca aacccttgcg gccgtcaccc tggtgcaggc acagggtttc
     5821 gtaaaggtcg gcctcgtcgg atcgagcttc ggcgggctgg tgtcgatcct taccgctgca
     5881 caacggcggg acattgcctg cctagccttg aaatgcccgg tggtagattt tgccgaagaa
     5941 cttcgcttag aattcgggcc ggatgaactg gctcgctggc aagtcacggg cacgattccg
     6001 aacctcatgg gcggaccgga gcggatccga ttgaagtacg gcctgtatga agactccctt
     6061 caacagattg cctatgggcc ggccgctcag atcgtggcgc cgacattaat cgtccagggc
     6121 gcatgcgacg aactggtgcc gcttcatcaa agccagcgct tgttggacgc gctgcgaggt
     6181 ccgaagcggc tggacatctt gccgcacgcg gaccatcaat ttacgcgggg cgaggatttc
     6241 agaaccatga ccaccgccat aaccgactgg ctcgtcgggt atctggtatg atacggaccg
     6301 actgcccggg caggagttga aggaacggcc atgatcacag aaggcgttca acaaaccctc
     6361 accgttctct atcccctcta caaaaatgaa gtgtatcagc ggcgcgagca catgatccgg
     6421 ttgacggcct ttggtgcgtt cggtttgatc gcgatgatct ttgcgctgct tctgagtccc
     6481 gtcaaggcgc ggctctctcc tgcggaaagc gtactattgg ccctcggcgg cctgacatgg
     6541 tgtgcactgt tttgcgggct ggtcctacaa caacagcatc ggcaccacct ggcgaaacag
     6601 gtcctgattc aactcgaaca ggcgctcggg ttttatgaag acggcctgtt tgtcgatcac
     6661 cagacactgt acccggccaa atggaaaacg gcgtggctga ccgaccggac cggcctgcac
     6721 tactatgcct ccctgtgtgg attgaccggc atctttctct ttgctctgat cctggtttaa
     6781 ggccgctcac ggcgcgctcg acgcgtcgtg actgctcacc gtctcttccc agtcctcccg
     6841 cacaaaatcc actttttctg gcctattaaa ggcttcggat agagaatgcc cttaccgcgg
     6901 ctgatgggta cggacagagc cgggcacgat cacgttcagt caagaaatat acccgtgatg
     6961 ttcactcacc ccaaatttca tcgctccctc attattgtgg cgctcctcac aatagtgaat
     7021 ctgatcggca tcgccacgtc cgtgctcgcg gaggagcagc agccggcgcg acatcacagt
     7081 ttgatccatc gccaggcatc cgatcttcta ccgctcgagc cggcaacgag cttatctcag
     7141 acggctgcgg ccccatcgac acctgctcca cgcccgctgc ctcggaaact cgccaaacga
     7201 ccagctgtac cgtcgcagac gacttcgacc acggcgctgc cggcaaccac agagacaacc
     7261 aaagccggca gcgagagcca tgcatcgatg cctgcgaccg gcctggcatc gacaggctcc
     7321 tcggtgccga gcgcgcccgc gacagcttcg agtgcctcga ctacactgcc gttgacgaat
     7381 gccgccccaa tgaccaaagc cgctgtgggt ggcctcgcga acacaggaac ggtttcctcg
     7441 atttcttcag gaagccatgg gacgggcgga cgcggactgc aacgcatttc tggtcaacta
     7501 ccggtttctc aaatgattgc gccgtcggtg acggtctctt cacctccacc accatcagct
     7561 ccgcggcaat cctcggcccc ttcctcatca cagccatcga cggccttgcc gcctccgccg
     7621 gcgtcgacat cgcctggaac aggaagcgct accttgagct ggacatcgaa cagtgaagcc
     7681 gatctcgccg ggtataaact ctatgtgggg acggcgccag gccactatac ctacgccgga
     7741 tccccgttcg tcatcggcct catgggcagc tacacggtct caggccttcc gatggggcaa
     7801 acctactact tcgccatctc agccttcgac tcgtctggta atgaaagcgg actctcttcg
     7861 gaagtcagca agagcatcta ctagcgactg ctggaaccgc atccaccgca ctgtctctca
     7921 tcgattactt cttctcagac atcgcgcagc ggaagcctgt ataactgttt ttggtgtccg
     7981 gcaggagctt aaagcggccg taggtcagca gatacttggg caggtcggac caggatcctc
     8041 cgcgcaggac tttgaattgg ccttgaatcg ggcccggggg atcacggtcc ggtgaaatct
     8101 cataatagtt cgtgccatac caatcctgga cccattcata agcgttgcct gccatgtggt
     8161 agagcccata agggcttttg ccctgctcgt gcgagcggac cggcaacagg acttggttgt
     8221 aactaaagcg ggcgcccagc gcaaaatttg ccaggttcgg ggtcgggacc tggttgcccc
     8281 aaggatatag ccgaccatct gttcctctcg cggctttttc ccattcggcc tcggtcggaa
     8341 gtcgtttgcc cttccaccgg cagaaggcgt ccgcatcatg ccaatccact ccgataaccg
     8401 ggcggtcggc gtgctgcatc agatccaccg tctcccactg ccagggcgcg gcatgcttgg
     8461 tcgccgccag aaacgccgcg tactgagcgg tcgtcacttc ctcagcatca atggaaaacc
     8521 catcgagcca gacgcgatga gctgggcgct catcttcgag tgcctgggta ccgtccgacc
     8581 ccatcacgaa tggaccggct ggaacctcaa ccatcggcgt gtcaggtatc ggactcgtca
     8641 tcaatgattt ggattttctc agccggtcga gctgcgccag actgaccgtt gcgtcacaaa
     8701 ggagaaggaa gacaataggg atggtcaaca cgatcaatac actcacccac cgacgccggc
     8761 gcgccgggac gcgccttttg gcgggcaagt ccgcagggtg gatctctgac gaagtgtctc
     8821 gcgggaatct gccgcagcca aaaaaacgac tgagaacgca gctcgggcaa ggatgcgcag
     8881 ccgcgcgagg gggctgggcg ggtgagaagg ggcaagggcg cgtctcggcg cggccagggt
     8941 gggtgggtga gaacaaaatc gtttccacca tcccttcagt aga
//
LOCUS       DASTQY010000349         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1077627, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000349 DASTQY010000000
VERSION     DASTQY010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077627"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..373
                     /locus_tag="VFR82_11455"
     CDS             146..373
                     /locus_tag="VFR82_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502257.1"
                     /translation="MKVFALACAACVLMGCGTIGSPIPPEDVGLAPLIERQKRQDALD
                     AKKLDAEESQTFMEPQGQDVDLPPLRPVGTR"
     gene            426..>2054
                     /locus_tag="VFR82_11460"
     CDS             426..>2054
                     /locus_tag="VFR82_11460"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU4502258.1"
                     /translation="MALIQPMRQGQLLKPVRGKRLLLVDPYPRNSQYHLTASERRAVW
                     FPKLSLPTIAAYTPDSWDVDLVDEAVQDIDFDDPCDMVGLSIMTCYAPRAYEIAKEFR
                     KRGKTVVMGGVHPTYCPDEALQHADAIVCGEAEDLWPRLVADYEAGMMQRVYKMTNFP
                     SLEHYKAPRVELLSPDSYMTRQCSFTTRGCHFDCEFCSVSPFNGKTTRRRPVPEVITE
                     LQNVQRWIRSELVERIRDEPLWNAFLAALKIRIGLDDGSIVAFVDDLHNSNRAYCREL
                     WTALKPLKIKWGCQSTLFLGDDEEMVKLAAESGCVSVFVGMESLDEDSLDETNKPFNR
                     VQKFSQEIQMFHKYGIMVNPGIVFGFDNDDEAVFERAVDFLKKNQVELAYFNVLTPLP
                     GTALFDRYNNAGRIFDRDWSKYDGKHVVFQPSRMTPEQLQEGFYWANHQFYSLPNIWY
                     RLSGTKQRFIARWEMNREFRKLVKRSCPKGRLSPLASVLKNLQAKLPVLDTDHLIPSA
                     LHALKQRFDPKAPSTQQLTLNIKVKRHDKFAALFM"
BASE COUNT          477 a          545 c          593 g          439 t
ORIGIN      
        1 tgcgacagtt ttccgagcgg tttgaccagg gggtattcac ccggttaacc gtggaagccg
       61 cgatcgatcg taaagcgcaa atcggaggca cagctcgagg ccaggaagag cagcggataa
      121 gagaattgga gcggatgtta agctcatgaa ggtcttcgcg cttgcatgcg ccgcctgcgt
      181 cttgatgggc tgtggaacga tcgggtcgcc gattcctccc gaggacgtcg gtttggcgcc
      241 gttgattgaa cgccaaaaga gacaggacgc attggacgcc aagaagcttg acgctgagga
      301 atcgcagaca ttcatggaac cccagggtca agatgttgac ctcccgccat tgcggccggt
      361 ggggacgcgc tagggttacg taacacacat tttttgtact cattacagaa gtggaggagc
      421 gactgatggc actaatccaa ccaatgcgac aagggcaatt gctgaagccg gtgcgaggca
      481 aacggctcct gttggtcgat ccttacccgc gcaatagcca gtaccatctg acggcgtccg
      541 agcgacgagc tgtctggttc ccgaagctga gcctgccgac cattgcagcg tacacccccg
      601 actcctggga tgtagatctc gtcgatgaag ccgttcaaga catcgatttt gacgatccat
      661 gcgacatggt cggcctctcc atcatgacct gctatgcgcc acgagcctac gaaattgcaa
      721 aggaattccg gaagcggggg aaaacagtcg tgatgggcgg ggtccacccc acgtattgtc
      781 ctgacgaagc cctgcagcac gcggatgcga ttgtctgcgg tgaggccgag gacttgtggc
      841 ctcgccttgt tgccgattat gaagccggga tgatgcagcg cgtgtacaag atgacgaact
      901 tcccttctct cgagcactat aaggccccgc gtgtcgaatt gttgagtccg gattcctaca
      961 tgaccagaca atgcagcttt acgacgagag gctgccactt cgactgtgag ttttgcagcg
     1021 tgtcgccgtt taacggcaag acgacgaggc gccggccggt ccccgaggtc atcacggaac
     1081 tccagaacgt acaacggtgg atccggagtg agctcgttga acggatccgc gacgaaccgc
     1141 tctggaatgc gtttctcgct gccctgaaaa tacgaatagg gcttgatgat ggaagcatcg
     1201 tcgcgttcgt cgacgacctg cacaacagca atcgggcgta ctgccgggaa ctctggaccg
     1261 ctctgaagcc gctcaagatc aagtggggct gtcaatccac cctgttcttg ggggacgacg
     1321 aagagatggt aaagctggct gcggaaagcg gttgtgtctc tgtgttcgtt ggcatggaat
     1381 cgctcgacga agacagtcta gatgagacga acaagccgtt caatcgggtg cagaagtttt
     1441 ctcaggaaat tcagatgttc cataagtacg gcatcatggt gaaccccggt atcgtgtttg
     1501 ggttcgacaa tgatgatgag gcggtattcg aacgagccgt cgatttcctg aagaaaaacc
     1561 aggtggagct cgcctacttt aatgtgctga ctccgttgcc aggcacggcg ctgttcgatc
     1621 ggtataacaa tgcgggtcgc attttcgacc gtgattggtc aaagtatgac ggcaaacacg
     1681 tcgtgtttca gcccagccgg atgactcctg agcaacttca agagggattt tactgggcca
     1741 accaccaatt ttattcactg ccgaacattt ggtatcggct gtccggcacg aaacagcggt
     1801 tcatcgcacg atgggaaatg aaccgggaat tccgcaagtt ggtgaaacgc agctgtccca
     1861 aagggagatt gtctccgctc gcctccgtac tcaagaatct ccaggccaag ctgcccgtac
     1921 tggataccga tcacctgatt cccagcgcct tgcatgcgct caagcagcgg ttcgacccca
     1981 aggcaccgtc cactcaacag ttgacgctca acatcaaggt caaacgacat gataagtttg
     2041 ccgctctgtt catg
//
LOCUS       DASTQY010000350        17900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_668546, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000350 DASTQY010000000
VERSION     DASTQY010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 17900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17900
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668546"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VFR82_11465"
     CDS             complement(<1..566)
                     /locus_tag="VFR82_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502259.1"
                     /translation="MRRLLGRTRWASITAFFSAALIAAGLSFTAAPAQAAFELPEGEK
                     ITNLPAIPRAMPQKEAYELYDPKIGKNFDLKNFWMRADLRVRPEMRNNVCFGGGAPVG
                     GACNGVAAIGAAPGARTANPGTKANDAYVQQWVRLGLGYDVSPDVNFYMEIIDAAVWG
                     GNGNQLNAGNGGDPLNHNGTSTGTGING"
     gene            complement(888..1187)
                     /locus_tag="VFR82_11470"
     CDS             complement(888..1187)
                     /locus_tag="VFR82_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU4502260.1"
                     /translation="MFGLGAGEILIILVIAFLLFGPKQLPEVGRQVGKAVKGFKETAE
                     DLRKSVEPELNMIQQEVKMVEQDFQASMKEAEEEINQATTPADKPGGSSGTGNQA"
     gene            complement(1271..1534)
                     /gene="tatA"
                     /locus_tag="VFR82_11475"
     CDS             complement(1271..1534)
                     /gene="tatA"
                     /locus_tag="VFR82_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU4502261.1"
                     /translation="MFGSFGWMELLLILIIVLIIFGAGKIPQLGEGLGKAIKGFKKSV
                     HEADAIDVTPPPVEPVPGQPTPMAQGQAPQSAPAQPEQQVKQG"
     gene            complement(1703..3133)
                     /locus_tag="VFR82_11480"
     CDS             complement(1703..3133)
                     /locus_tag="VFR82_11480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU4502262.1"
                     /translation="MLQDADALPQLIGDWKAVDQWQAHINQLFYRFRGDQVRNFYQTF
                     ASADYRLAHALAADYFEQVTRRDKVSQPQQPAAGSRPLTVVELGPGNGNLAASFLSHL
                     HTLDREGRVYPRVRYVLVDWEQTVLDGAWNYPGLAEHRDRVQMVRSTIDHIEGLADGA
                     VDRIFCNELWNDLATKLMTRQGGDIEEEFLRPNLSDVLHAKIEDWAGFIRAFEAKDAD
                     QLKQFPPFFDDLVWEREYRPVEWKDVPFRKTITEFLRRIDDQVVVPVNIGAYLTMREA
                     KRLLAPDAVGFSSFDAGAADHKVLNDPDKPCSGQFGGQQSFMVNFALAEAVAKQIGGV
                     TASIEPQREFIGRSLGTNVLTLMDLLATHPTAGHQLEPWEQDRLILKTIKALNLTYES
                     PYATKMDFPIRANVAFEAREALQALAEELKPAGLPDTVSYLTEEELTRAARDLEDVGY
                     DPQMFHIALTAPPAPVDYYHLSIRPS"
     gene            3155..3799
                     /locus_tag="VFR82_11485"
     CDS             3155..3799
                     /locus_tag="VFR82_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502263.1"
                     /translation="MAEILADGFKWLQPALLLDSPRLAVYERFMGLCLAIWVPLLIWW
                     LASPPLLSAGESPMTDPSSELESAPQEQGGYDFDAPPQGLFRSITMAEGFEEEIGFRR
                     THEIVPVKPTERFGPDVSGIFIVFTLHQHYQAFQVFGRCFPESVSGLDPHTMVGQDVM
                     YIALEDESGYLKLSSPQGRWKPGRYKVEIHAGEQVNEVSLMGTMRFTVAASHEP"
     gene            3828..5531
                     /locus_tag="VFR82_11490"
     CDS             3828..5531
                     /locus_tag="VFR82_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4502264.1"
                     /translation="MTDPATHTSFPQYRLTNTVQDIITQLGLLIRLERKMFGIIASYA
                     VTIGMFMLCVPIAVQELVSTFSFAIEPIMILTLTLVVGSALTGAAAFRILQARAVETL
                     QQRIYARIAIAFTETLPKIREEAFLPQHAHRFAEADLLTRALVAMVADLFYVAVVGSV
                     GMSLLVFFHPYFLLYNVILVVGFVFLLTVFGKGGFLITLEMSRLNYEIYHWMQNIAAN
                     LPHLRAVGNSPYLIRKTDELTKAYAEVRQRRSDLLTGRQYKAAALWQAVGHAGLIGTA
                     GMLVSQGQLTVGQFAAAELVAGNLLLNMDTLARRMVHMFFAFVSFRELNAFFSLPQDE
                     TASRTVVPLSQFGLAGIKVTARNLSFAYAGAPLLFEHFHLDVAPGEKVVVLCQSNMQK
                     TALSRILAGLLAPSSGVVRYNDMNLVEVSLDSIDTCRSLMLDSHPTLLEGTIEDNITL
                     GRQSIGYEDVQWALEFVELDEEIDRMQEGLTTNVTGQSTQFTLSQILRLLLARAIVTR
                     PHVLIVEGTLHSMAPSLREVILRRLCSKDEPWSIIFVSNDPAFSSYVDRQITLEHLPS
                     V"
     gene            5607..6341
                     /locus_tag="VFR82_11495"
     CDS             5607..6341
                     /locus_tag="VFR82_11495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4502265.1"
                     /translation="MTHSSQPWISVVVPIKDERDNLVSLTDQLMKVLHARGESQHTPF
                     EIIYVDDGSTDGSSQMLDRIAAEHPSVIVLHFDRNYGQSSAFDAGFKRSTGMLVVTMD
                     GDLQNDPADIDTLLAYSDKYDLVCGWRTERHDSLTRKISSRVANAVRSAVTGDRVHDT
                     GCSLKVFRREVIDRLQLFVGMHRFFPALALMHGFTVTEVPVRHHPRMHGVSKYGVGNR
                     LFKGLYDLLAVRWMQDRVLKYRFRDK"
     gene            6345..6668
                     /locus_tag="VFR82_11500"
     CDS             6345..6668
                     /locus_tag="VFR82_11500"
                     /inference="COORDINATES: protein motif:HMM:NF019214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HEU4502266.1"
                     /translation="MLPDSMFSVEILWLAIGFLGQGLFFGRWLVQWIVSERKAESRMP
                     IAFWYLSLVGGLITLAYAIYRRDPVFIAGQGIGALVYIRNLMLIYRQGSHQHAGPVPS
                     VGKPQ"
     gene            6675..8459
                     /locus_tag="VFR82_11505"
     CDS             6675..8459
                     /locus_tag="VFR82_11505"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU4502267.1"
                     /translation="MHAPVSPISGHIVNLAILILLSGLLFFLGLGSMGLTDRDEGRNA
                     EAGREMLETGNWVSPTFNYEPRFAKPALVYWLMSLSYRWLGIDELAARLPSAAFGVGL
                     IVLQYLFISRFCGAVVGLLGALMLLLNIEIIGLSRMALTDSVLICFTTLSLYSFWLGF
                     HGRQRERGWRWIFYLAMGMATLAKGPVGFLVPLVTIALYLTVTKQWRRFWREGTPVAG
                     LLLFAVVALPWYLIMWSLHGTQYAASAQANTVGRFLSPMEGHGFTILFYVPVLLLGFF
                     PWSGWLPFAWYQAYRSWRERRGEISISSDAHQSTPTEPASAFHSTTVSLEWFAAAWVF
                     GVFMFFSLSSTRLAHYIGPLFPAAALLTATYWHRSLHNVQTRGRRASIHTMMIMGYLL
                     AIGFASLPSLYPSFAGRLVKEFPLATTMTLGSGPYVASAVLLVGMALVGYFGLSERRR
                     AGAFWAAGGSLALLALTIIQLVLPGLNRYFITPPQILSYAAGLNLMPTDRLIVYGSTR
                     PSNVFYARRKVLFVSEGEEATIREALSQPGQTMVVLPETFKSKLPAEASTLMPILRQH
                     GYLLLASRSMVVIPEGTTSPPARAVPGH"
     gene            complement(8462..9562)
                     /locus_tag="VFR82_11510"
     CDS             complement(8462..9562)
                     /locus_tag="VFR82_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU4502268.1"
                     /translation="MNDASDGAAAESSRLVPATYASMLSFTMCCTALALTLFRLPVFD
                     FPLTRYVRSLNDFHIDHLRHPWLAWLSDVGDQVGGSEFLLCVSALLLAVGYALKLKAW
                     TIAGWETLIVHAVAGLGSTTLKHVVGRARPKFMHVGVSEFSPFAGNGWDSFPSGHTTC
                     SFAIATVLAVRFPKLRWVVFGIALAISASRLFRGSHFLTDIIAGAVLGVLIGAVIPRR
                     QEWRRALATALYAVTPPLAVLFAVITAIGHPLSDEWTAKALTEAGLLLAWVALLILLL
                     MKVRPALFPIRATRAVILALIGLGLGMCSGSVWVTAVALLTGFAHWLRAGRQGRPDEP
                     PHFPWYREAALGLGLLLSLYTMLELRGALPLG"
     gene            complement(9562..10683)
                     /locus_tag="VFR82_11515"
     CDS             complement(9562..10683)
                     /locus_tag="VFR82_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502269.1"
                     /translation="MKLSLLTRALLLTVVFVATFLHCSSAGESSTKPTLTGGTERHLK
                     NIRQLTFGRQNAEAYFSFDGTKLIFQSTNNWMKDSFAATLKPAEEGLGCYQMYVMDLE
                     SGAIRLVSTGTGATTCGYFFPGDRRVLYSSTYATGPNCPPKPKRDGAYRWALDDYDVY
                     AVRVDGQEMQRLTSTPGYDAEATISPDGKTIVWTSLKDGDLDLYAMNLDGTKSRRLTE
                     EVGYDGGAFFSPDSKRIVYRASHPSDEVELSRYKELLGQRLIEPGQLEIFVMNADGSG
                     KRQVTTNGASNFSPFFHPDGKRIIFSSNVETRGEGGRPSFHLFLIADDGTGLERLTVE
                     GHFNSFPMFSPDGTRLVWVSDRHAKVPGEFNVFLADWIP"
     gene            complement(10661..12901)
                     /locus_tag="VFR82_11520"
     CDS             complement(10661..12901)
                     /locus_tag="VFR82_11520"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEU4502270.1"
                     /translation="MGKSCLRPAMAALWLFFCLSPSVVTTATPQPFAAPFISRHDLFV
                     QIDPERHALLAVDRLTINAIPGRPLRLSLAGTLHLDRLVLSRSATDDSGRDLPVQTEH
                     EAAAPFGQYITLPAALVTAGVMTVTAHYHGAIDDPPKEPRHLRFVTPSETAGHIGMEG
                     VYLSSETRWYLDVPESLSRYRLRVALPSGWTAVAQGQRRSSVPCPAELCRDESLTLAE
                     WETVEESEALTLVANRFVETTREWSSSSGQAVRLATYLFPEDAELADEYLDATARYLD
                     VYMSLLGPYPFESFAVVENFFASGLGMPSFTLLGSGVIKRHYVQPYALGHEIVHSWIG
                     NSVFNHVDRGNWVEGLTTYLANYYWHEWSGDHEQARDQRHLMLRGYNLHVPPERDYPL
                     GQFTRKQDERDNAIGYQKAAMVFHLLRQEVGEVSFWRSLKTLVAQYRGRYADWHDVER
                     IFAETAAKDLRWFFAQWVEGTGAPALTLKNVTAHPLARDADGRFTLSGSIAQTGPVFR
                     SPLPILVGMSNSSQHLLWAQLSGADTLFKSDLPFKPLTIELDPEAMVLRRLARRDLPP
                     VLNHYVTDTKRSIIVALAGSDTDSHPFQDVVKRIKSQDGQKAPAQRTAITTLAENALL
                     PGEGSVLILGSPASRLTLQPLVSTHCGTRVQLRDGGVTVAEKTYDGPGIALVASCHRE
                     DRPGSVVTSVYALTPQSATTVARLLFFYGWNSYVVFQDGKAIARGEWDAQHSQEVSFD
                     ETQSAH"
     gene            complement(12912..13694)
                     /locus_tag="VFR82_11525"
     CDS             complement(12912..13694)
                     /locus_tag="VFR82_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502271.1"
                     /translation="MVKILVIDDDRMNCDLLQAVLTRHGYDVYSATSGPEGLNLFRQQ
                     NPRVTILDLRMPGMDGLTVLKEIRAIDPHAPVIILGGGATEIQENQARTLRVTDFVRK
                     GLSLDVLVEGVNRVVQQPAKKTEGATAQPGNAVAETGETVLIVDDEQLVCDLLVQFLS
                     LRGYRALGVKDGAQALSMIEQTPPDLILLDLMLPGMNGVEVLRRLRTNNFAGAVIIVT
                     GSYDEELLQDAWSLRPQEVISKPIDLDRLLSIIQLVLVCREC"
     gene            complement(13798..14727)
                     /gene="ftsY"
                     /locus_tag="VFR82_11530"
     CDS             complement(13798..14727)
                     /gene="ftsY"
                     /locus_tag="VFR82_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU4502272.1"
                     /translation="MGWFQRLSEGLSKTRSVMRGSLDRLLGRAVDPALLDDFEAALIT
                     SDLGAAVAERVISRLKEALQGTDASQATHVHATLRQTLLEILTPASGPTFEQLIANGP
                     KPFVVLVVGVNGVGKTTSMAKLAQRLVQSKRVPLLVAADTFRAAAIDQLQVWADRIGV
                     EMIRHRHGADPAAVAYDGIAAAKARQADVVLIDTAGRLHTKSNLMEELRKVSRVIGQA
                     LPGAPHEVLLVLDATLGQNALSQARQFYQTVGVTGLALTKLDGTAKGGIVVAIADELK
                     LPVRLIGVGEAVDDLQDFQPEAFVDALLGAAID"
     gene            complement(14744..15250)
                     /gene="ybeY"
                     /locus_tag="VFR82_11535"
     CDS             complement(14744..15250)
                     /gene="ybeY"
                     /locus_tag="VFR82_11535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU4502273.1"
                     /translation="MGPAALMPVSLQVHVRSIRFGRHSILRLAQAVLNLIGESSADLG
                     LSFVGDRRMRRLNHRFRGKDRSTDVLAFALREARTPVFHVSRRVLGDIVISVPTALRQ
                     AKEGRRSLEEEIVALLIHGALHLCGYDHERGQAESRRMRRKERTVRLALGRIACFAGR
                     RPYVRKSS"
     gene            complement(15232..16287)
                     /locus_tag="VFR82_11540"
     CDS             complement(15232..16287)
                     /locus_tag="VFR82_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU4502274.1"
                     /translation="MRKLKLREGTNTAVLFGHHDRHLKLIEEDLGVRLSARGEELTLD
                     GNPEATRYAERVLTELAGLANDGMLIQPDDVTHALTALRQSPETPLKELLSNATTIVT
                     KKRFVAPKTPTQKTYIEAIETHDIVIGIGPAGTGKTYLAMAMAVSALMKREVSRIILA
                     RPAVEAGEKLGFLPGDMIAKVNPYLRPLYDALFDMMDMERATRAIERGDIEIAPLAFM
                     RGRTLNDSFVILDEAQNATAEQMKMFLTRLGFNSKAVVTGDITQVDLPPDRVSGLIEV
                     RDILRHVEGIRFVYFDERDVVRHKLVQDIIKAYDQHEQTEQAHPQAAQGGRRQSSHTR
                     PSSSLPSATKGSWGQPH"
     gene            complement(16521..>17900)
                     /locus_tag="VFR82_11545"
     CDS             complement(16521..>17900)
                     /locus_tag="VFR82_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEU4502275.1"
                     /translation="SFPGMTIRYSVGLRRDIEQFLGATMEQQGSASTIGTILSELEED
                     LPLDPVLERPSGPIDENDSLIVRLTNHLISEADRLGASDLHFEPSTSKREMNIRFRVD
                     GTCFSYTKVPTAYRRALISRLKIMANLDIAERRKPQDGKIRFKLDAQRSLELRVATLP
                     TAGGNEDAVLRLLTAKEPMPLEAMELTPSALQTVKAMAEKPHGIILCVGPTGSGKTTL
                     LHAILKHINTEERKIWTAEDPIEITQEGLRQLQIHQKIGLDFATAMRSFLRADPDVIM
                     IGEMRDKETADIAIEASLTGHLVLSTLHTNSAVETVIRLLDLGCDPFNFADAMLGVLA
                     TRLCKRICGVCKESYRPSPEEYQELVEGYGAEGWPLSHAAQDDLQLFRGRGCEYCRHS
                     GFKGRVPLHELLVGTDEIKQLIQSRSRTAAMLIKAVEDGMLTLLQDGIRKVLQGLTTY
                     RQVRAVASK"
BASE COUNT         3765 a         5233 c         4959 g         3943 t
ORIGIN      
        1 tttccattga taccggttcc tgtggatgta ccattgtggt tgagtggatc accgccgttg
       61 cccgcattga gctggttgcc attcccaccc catacggcgg catcgatgat ttccatataa
      121 aaattcacgt ccggcgagac gtcatagcct aagcccaacc gaacccattg ctggacatac
      181 gcatcgttag ctttggtccc aggattggcg gtacgggctc ctggagccgc accgatcgcc
      241 gccacaccgt tacaggcccc gcccactggc gcgccgccgc cgaagcagac gttgttgcgc
      301 atttcggggc gcacgcggag gtccgcgcgc atccagaagt tcttaagatc gaagtttttc
      361 ccgattttag gatcgtagag ctcgtaggct tctttttggg gcatcgcacg ggggatggcc
      421 gggaggttcg tgatcttttc tccctccggc aactcgaagg ccgcttgggc tggtgctgct
      481 gtgaacgaca ggcccgccgc tatgagggcg gcgctgaaga aggcggtgat gctggcccac
      541 cgtgtccgac ctagcaatct tctcataaca ttcctcctgt gaattggatt acagccttca
      601 ccgttccggg actctaccgg tcctggctgc cgacttgaca tccaactgcg ctcgcactga
      661 caaacttctt tttattcgtt tgccggtttg gaaagccgtg tgcccacgct ttcctggaac
      721 cttactgacc catgacacgt ggagtgtcta tcaatactcc ttgttttcct agtaattttg
      781 ctctttttaa atacgaccct tcgccctcac tgtcctgcca ccgccgatcg ggtcgagagg
      841 cggtcacccc cttccctgta tcatcgaaca gcccgactgc tcaggtccta cgcctgattg
      901 cccgttcccg aagatccccc cggcttgtca gccggcgtag tggcctggtt gatttcctcc
      961 tctgcttctt tcatcgaggc ctggaaatcc tgttcgacca tcttgacctc ctgctgaatc
     1021 atgttgagtt caggctcgac tgatttccga aggtcttcgg ccgtttcctt aaagcctttc
     1081 accgctttgc ccacttgacg cccgacttcg ggcaactgct tggggccaaa cagcaaaaac
     1141 gcgatgacca ggataatgag tatttcgccg gcacctaatc cgaacataag tggtcatacg
     1201 tcatgcgtga aacgtaatgc gtggatctcc ttcatccgct tcacagctga cgcacaacgc
     1261 ctcacgtcag ttatccttgc ttgacttgct gctccggttg agccggggcg gactggggag
     1321 cctgtccttg tgccataggt gtcggctggc ccggcaccgg ctcgaccggg ggcggcgtca
     1381 catcaatggc atcggcttca tggacactct tcttaaatcc cttaatggcc ttccctagtc
     1441 cttcgcccaa ttgaggaatt ttgccggctc cgaaaatgat gagcacgatg atcaggatga
     1501 gcagcagttc catccagccg aaagagccga acatcagtag atttctcttt cttccatgtc
     1561 tccccatgac acggcaaacg caacattacg taacccaaaa ggtggggatc cttctcaaat
     1621 tgggtcgaag gctatagaaa tacgtgagat tagtcaagtt taatttacgg aagaggccat
     1681 cgggaggggc ttgaacccca aatcaggaag ggcggatcga caagtgatag taatcgaccg
     1741 gagcgggagg ggcagtcaga gcgatgtgaa acatctgcgg atcatacccc acgtcttcaa
     1801 ggtctcttgc cgcacgtgtc aattcctcct cggtgaggta ggaaacagta tcgggtagtc
     1861 ccgccggctt gagctcttcg gccaatgcct gcagggcctc tcgggcctca aacgctacgt
     1921 ttgcgcggat tgggaaatcc atcttcgtcg cataaggact ttcatacgtg aggttgagcg
     1981 ccttgatagt cttgagaatc aggcgatcct gctcccatgg ctcgagctga tggcctgccg
     2041 tcggatgcgt ggctaggaga tccatgagcg tcaacacatt cgttcccaag ctccggccga
     2101 taaattcccg ctgaggttcg atcgaggccg tgacgccgcc aatttgcttt gcgactgcct
     2161 ccgccagagc gaaattcacc atgaagcttt gctgccctcc gaactgaccg gagcagggtt
     2221 tgtccgggtc gtttaggacc ttgtgatcgg cggctccggc gtcgaaactg ctgaatccaa
     2281 ccgcatccgg cgcgagaagg cgtttcgctt ctctcatggt gagatacgcg ccgatattga
     2341 caggtaccac cacctgatcg tcgatgcgac gcaggaactc tgtgatcgtt tttctaaacg
     2401 gaacatcttt ccattctacc ggccggtatt cgcgctccca cacaagatcg tcgaaaaagg
     2461 gaggaaattg cttcaattga tctgcatcct tggcttcgaa ggcacgaata aatcccgccc
     2521 agtcctcgat tttcgcatgc aagacatcac tcaaattggg gcgaagaaat tcctcttcaa
     2581 tgtcgccgcc ttgccgggtc atcagcttgg tggccaagtc gttccaaagt tcattacaaa
     2641 agatgcgatc cactgcccca tcggcgaggc cctcgatgtg atcgatcgtc gatctgacca
     2701 tctggaccct gtcacgatgt tctgccagcc ccggatagtt ccatgctccg tccaacaccg
     2761 tctgctccca atcgacaaga acgtatcgga ctcgcgggta cacccgccct tcgcggtcga
     2821 gtgtgtgaag atggctgaga aacgaagccg ccaaattgcc attgccgggg ccgagttcga
     2881 caaccgtcaa gggtcgggaa ccggctgcag gctgctgggg ctgtgagacc ttgtcacgcc
     2941 tggttacctg ttcaaagtag tctgccgcca acgcatgggc taggcggtaa tcggcggagg
     3001 cgaacgtctg ataaaaatta cgaacttgat cgcctcggaa gcggtaaaag agttgattga
     3061 tgtgcgcctg ccattggtcg acggccttcc aatcgccgat gagctgaggt aaagcgtccg
     3121 cgtcttgcag cataatccgt ggagattcct tgtcgtggcc gaaattctag cagatgggtt
     3181 taaatggctg caaccggcgc tgctccttga cagtcccagg ctggcagtgt acgaacgatt
     3241 tatgggtctc tgtctggcta tctgggttcc cctgcttatc tggtggctgg catctccccc
     3301 gctcctctct gctggcgaaa gccctatgac agatccgagt tctgaattag aatccgcgcc
     3361 acaagaacag ggcggttacg atttcgatgc gcctcctcaa gggctgttcc ggagcatcac
     3421 gatggctgaa gggtttgagg aagagatagg ctttcgccgc acgcacgaaa ttgtcccggt
     3481 caagccgacc gagcggttcg gcccagatgt gtcgggcatt ttcatcgttt tcactctgca
     3541 tcagcactac caggcctttc aggtcttcgg tcgctgcttt cccgaatcgg tttccgggct
     3601 cgatccgcac acgatggtcg gccaggatgt gatgtacatc gcccttgagg atgagtcagg
     3661 ctatttgaag ctatcgtccc cgcaaggccg ttggaagcca ggccggtaca aagtagagat
     3721 tcacgcaggg gaacaggtca atgaggtcag cctcatgggt accatgcgct tcactgtggc
     3781 cgcctctcac gaaccatgac aaagccgaag gccagaattg gccgaccatg acggatcccg
     3841 ctacgcacac ctcctttcca caatatcggc tcacgaacac cgtccaagat atcattactc
     3901 aactcggact cctcatccgc ctcgaacgga agatgttcgg catcattgcg tcgtatgccg
     3961 tcaccatcgg catgttcatg ctgtgcgtgc ccattgcggt acaggaactg gtcagtacat
     4021 tttcctttgc catcgaaccc attatgatcc tgacgcttac cctcgtggtc gggtcggcac
     4081 tcacaggcgc cgccgccttt cgtatcttgc aagcccgggc ggtcgagacc ttgcaacagc
     4141 gcatttacgc gcgtatcgcg atcgcattca cggagacgct tcccaaaatt cgggaagaag
     4201 cattccttcc gcagcatgcc catcggttcg ccgaggcgga cctgttgaca cgcgcgctcg
     4261 tcgcgatggt tgccgatctc ttctatgtgg cggtcgtggg ctccgttggc atgtcgctcc
     4321 tcgtcttttt ccatccgtat tttttgctct acaacgtcat cctcgttgtc ggattcgtct
     4381 ttcttctgac cgtgtttggg aaaggcgggt tcctcattac actggagatg tcccggctca
     4441 actacgagat ttatcattgg atgcagaata tcgccgccaa cctgccgcat ttgcgggcgg
     4501 tcggcaacag cccgtatcta attcggaaaa cggacgaact gacgaaggct tatgccgaag
     4561 ttcgccaacg gcggtcggat ctcttgaccg gacggcaata taaggccgca gccttgtggc
     4621 aggcggttgg acatgccggg ttgatcggaa ccgcaggcat gctggtatca caaggccagc
     4681 tcaccgtcgg ccagtttgcg gcggccgagc tggttgccgg caacctcctc ctcaacatgg
     4741 ataccctggc ccgccggatg gtccacatgt tttttgcttt cgtctcgttt cgagaactga
     4801 acgcattttt ttcgcttcct caagacgaga ctgcctctcg aaccgtcgtg cccctcagtc
     4861 aattcggcct ggcaggcatc aaggtgacgg ccagaaactt atcctttgcc tatgctggcg
     4921 ctcctctcct ctttgagcac tttcacctcg acgtcgcccc gggtgagaag gtggtggtgc
     4981 tgtgccagtc caacatgcaa aaaaccgcgc tctccaggat cctggccgga ctcctcgcgc
     5041 catcaagcgg tgtggtgcgg tacaacgaca tgaacctcgt cgaggtcagc ctcgactcga
     5101 tcgatacgtg ccgcagtctg atgctggatt cgcatcctac cttgctcgaa ggcacgatcg
     5161 aggacaacat cacgctcgga cggcaatcga ttgggtacga ggacgtccaa tgggcccttg
     5221 aattcgtgga actcgatgaa gaaatcgatc ggatgcagga agggctcacg accaacgtta
     5281 ctggccagag cacccagttc acgttgagcc agattctcag gctgctcctc gcgcgtgcca
     5341 tcgtcaccag accgcatgtc ctgatcgtcg aaggcactct gcacagcatg gcaccctctt
     5401 tgcgggaagt gattttacgc cgcctatgtt ccaaggatga accatggtcc atcattttcg
     5461 tctctaatga ccccgcgttc agcagttatg tcgaccgcca gatcacgctc gaacacctgc
     5521 cttcggtttg aatctcccaa aggccttctg tacaatgccg tggtgattcc tcctcatggt
     5581 gtacgtgctg cgcgtttcca ctcctcatga cccactcatc ccagccatgg atctcggtag
     5641 tcgttcctat caaggacgaa cgggacaatc ttgtctcatt gactgaccag ctcatgaagg
     5701 tgttgcacgc tcgaggggaa tcgcagcaca ccccgttcga gatcatctat gtcgacgacg
     5761 gcagcaccga tggcagcagc caaatgttgg atcgcatagc cgcggaacac ccgtctgtca
     5821 tcgtgctgca tttcgatcgc aactacggac aatcctcggc attcgatgca ggattcaagc
     5881 gatcgaccgg catgctggtc gtgacgatgg acggcgacct tcagaacgat ccagccgaca
     5941 tcgacacgct gctggcctat tcagacaagt acgatctggt ctgcgggtgg cggaccgaac
     6001 ggcatgatag tctcacacgg aaaatttcgt cccgagtggc aaacgccgtg cgaagcgctg
     6061 tcacgggtga ccgtgttcac gacaccggat gctcgctcaa ggtattccgc cgggaggtca
     6121 tcgaccggct gcaacttttt gtcggcatgc accggttctt tcccgccctt gccctcatgc
     6181 acggattcac tgtgactgaa gtccccgtcc gtcatcaccc gcgcatgcac ggcgtatcga
     6241 aatacggtgt cggcaaccgg ctcttcaaag gactctacga cctgttggcc gttcgctgga
     6301 tgcaggatcg tgtcttgaaa taccgcttcc gcgacaagtg atcgatgcta ccggattcca
     6361 tgttctcagt tgaaatcctt tggcttgcca ttggtttcct cgggcaaggg ctgtttttcg
     6421 gtcgatggct cgttcaatgg atcgtctcgg agcgaaaagc cgaaagccgg atgccgatcg
     6481 ccttctggta tctgagcctg gtcggagggt tgattacatt ggcctacgcg atttaccggc
     6541 gtgatccggt ctttatcgcc ggacagggaa ttggagcact ggtctacatt cgaaacctca
     6601 tgctgattta tcgtcaagga tcccaccagc acgcaggacc ggttccttcg gtcggaaaac
     6661 ctcagtaacg cctcatgcac gcgcccgtct cgccgatatc cggacacatt gtgaacctgg
     6721 ccatcctgat cctcttgtca ggtctcctgt tttttctcgg attaggctcg atgggattga
     6781 ctgatcggga tgaggggcgc aacgccgaag cggggcgaga gatgctggaa acgggcaatt
     6841 gggtcagccc tactttcaac tacgagccac gttttgccaa gcccgctctc gtgtattggc
     6901 tgatgagctt gtcttaccgc tggttgggaa tcgatgaatt agccgcccga cttccgtcag
     6961 ccgcctttgg cgtaggtctg attgtgctgc aatacctctt tatcagccga ttctgtgggg
     7021 cagtggtggg cttgctcggc gccttgatgt tgctgttaaa catcgagatt atcggcctaa
     7081 gccgcatggc cctgaccgac agcgtgttga tctgtttcac gacactgtcc ttgtacagct
     7141 tttggctggg gttccatgga cggcagcgcg aacgcgggtg gcggtggatc ttctatctcg
     7201 cgatggggat ggccacactg gctaaagggc cggtcggatt tcttgttccg ctcgtgacca
     7261 ttgcgctgta tttgacggtg accaaacaat ggcgtcggtt ttggcgcgag gggactccgg
     7321 tggcggggct cttgctgttt gccgttgtgg cactcccctg gtatctcatc atgtggtctc
     7381 ttcacgggac gcagtacgcg gcatctgcgc aggccaatac ggtcggccgc ttcctcagcc
     7441 cgatggaagg ccatggtttc acaatcctgt tttatgtccc cgtcctgttg ttggggtttt
     7501 ttccgtggag cggctggctg ccttttgcct ggtatcaagc ctatcgaagt tggcgagaga
     7561 gaagaggcga aataagcatc tcgagcgacg cccatcagag tactcctact gaacccgctt
     7621 cagcgtttca cagtacaacg gtaagcttgg aatggttcgc ggcggcgtgg gtcttcggcg
     7681 tgtttatgtt tttttcgttg tcctccacca ggctggctca ctacatcgga ccgctgtttc
     7741 cagctgccgc gctgctcacg gccacttact ggcaccgtag ccttcacaat gtccagacaa
     7801 gaggccgacg cgcatcaatc cataccatga tgatcatggg ctatcttttg gcaattggat
     7861 ttgcgtcatt gccatccctg tatccttcgt ttgctggacg cctggtgaag gagtttccgc
     7921 tcgcgacgac gatgactctc gggagcgggc cgtatgtcgc ctccgctgtc ctgttggtcg
     7981 gaatggcgct ggtcggctac tttgggctga gtgaacgaag acgggctggg gcattctggg
     8041 ctgccggggg ttcactggca ttgttggcat tgaccattat ccaactggta ttaccgggcc
     8101 tcaatcggta tttcattacg ccgccccaaa ttctttctta tgctgccggg ctcaatctga
     8161 tgccgaccga tcggttgatt gtgtatggat caaccagacc ttcaaacgtg ttttatgcga
     8221 gacgcaaagt gctcttcgtc tcggaagggg aggaggcaac gattcgtgag gcactcagcc
     8281 agcctggtca gactatggtg gtgctaccgg agaccttcaa atccaagctg ccggcggaag
     8341 cctcgaccct catgcctatc cttcgacaac acggctatct cttgctcgcc agtcggtcga
     8401 tggtcgtcat tcctgaaggc accacctcgc ctccggctcg agccgttccc ggccattaaa
     8461 cctatcccaa cggcagggcg cctctcaatt caagcatcgt atacaacgac agcaacagtc
     8521 ccagtcctag cgccgcctcg cgataccagg ggaagtgagg gggctcgtcc ggccgtcctt
     8581 gtcgaccagc gcgcaaccaa tgcgcgaagc cagtcagaag ggcgaccgcc gtcacccaga
     8641 cggaccctga gcacatcccg aggccaaggc cgatcagcgc gagaataacg gctcgagtcg
     8701 cacggatagg aaagagagca ggtcgcacct tcatcaagag aagtatgagc agcgccaccc
     8761 aagccagcag caatcccgct tcagtcaacg cctttgcagt ccactcatca gaaaggggat
     8821 gcccgatggc ggtgatgacc gcaaaaagga ctgcaagtgg cggcgtcacg gcataaagcg
     8881 ccgttgccag tgctctgcgc cattcttgtc ggcgcgggat aacggcgccg atcagcacgc
     8941 caagaacggc acctgcgatg atgtcggtca agaaatgtga cccccggaaa agccggctcg
     9001 cggaaatagc caaggcgatg ccaaacacga cccaacggag cttagggaat cgcaccgcca
     9061 gcaccgttgc gattgcgaag gagcacgtgg tatggccgga cggaaacgaa tcccacccat
     9121 tgccggcgaa cggggaaaac tcagagacac ccacatgcat gaacttaggc ctggcacgac
     9181 ccacaacatg cttgagggta gtgctaccta ggccggccac agcgtgaaca atgagcgttt
     9241 cccaaccggc gatcgtccaa gccttgagct tgagcgcgta tccaaccgcc aagagcagag
     9301 cgctcacaca caacagaaat tcactaccgc ccacttgatc gccaacgtca ctgagccatg
     9361 ccagccatgg atgacggaga tggtcaatgt ggaagtcgtt gagagagcgc acatagcgag
     9421 tgagcggaaa atcgaacaca ggcaggcgga ataaggtcag cgcgagggcg gtgcaacaca
     9481 tggtgaacga cagcatcgac gcgtaggttg ccggcaccag tctcgacgat tctgctgccg
     9541 caccgtcaga cgcgtcattc atcacggaat ccagtcggcg agaaacacat tgaattcacc
     9601 gggaaccttt gcatgacggt ccgacaccca caccagccgc gtcccatcgg gagaaaacat
     9661 gggaaagctg ttgaaatgtc cttcaaccgt caggcgttcc aagccggttc cgtcatcagc
     9721 gatgaggaac agatggaaac ttggtctgcc tccctcgcct ctagtctcga cgttggatga
     9781 gaagattatt ctcttcccgt ccggatgaaa aaagggtgaa aagttggagg ccccattcgt
     9841 ggtgacctga cgcttcccgc tcccatcggc attcatgaca aaaatttcca attggccagg
     9901 ttcgattaac cgctgtccga gcaactcttt gtaccgtgac agctcgacct catcgcttgg
     9961 atgagaggcc cggtagacga tgcgtttgct gtcaggcgaa aaaaacgcgc caccgtcata
    10021 cccgacctcc tcggtcagac gacgagattt cgttccgtcc agattcatgg catacagatc
    10081 aaggtctcca tccttcaagg aggtccacac gatggttttc ccgtctggcg agattgtcgc
    10141 ttccgcatcg tagcctggtg tcgaggtcag acgctgcatc tcctgcccgt caacgcgaac
    10201 cgcatacacg tcatagtcgt ccaacgccca tcggtaggcc ccgtctcgtt tgggcttcgg
    10261 cgggcagttc ggtcccgtcg cgtatgtgga agaatagaga acacgccgat ctccagggaa
    10321 aaaatacccg cacgtcgtgg cgccggttcc tgtgctgacc aaccgaatag caccgctctc
    10381 gagatccatc acatacatct gatagcaccc cagaccctct tcggccggct tgagcgtcgc
    10441 agcaaaagaa tccttcatcc aattattcgt ggactgaaaa atcagcttgg tgccgtcgaa
    10501 cgaaaaatag gcttccgcat tctggcggcc gaaggtgagt tgccgaatgt tcttcaaatg
    10561 tcgttccgtg cctccggtca aagtcggttt cgtcgaagac tcgccggcgg acgagcaatg
    10621 aaggaaggtc gccacgaaga cgactgtcag aagcagcgcc ctagtgagca gactgagttt
    10681 catcgaacga tacctcctgg ctgtgctggg cgtcccattc gccgcgagca atcgccttgc
    10741 catcctgaaa gactacatag ctgttccatc cataaaaaaa caacagccgg gcaacggtgg
    10801 tggcggattg cggcgtcagg gcatacaccg atgtgacgac actgccagga cgatcttctc
    10861 gatgacagct tgcgacaagg gctatgcccg gaccgtcata ggtcttctct gcgacggtca
    10921 cacctccatc ccgaagctgc acacgagtgc cgcagtgagt actgacgagc ggctgaagcg
    10981 tcaaacggga ggccggactc cccaatatta gcactgaacc ttcaccaggc aacagagcat
    11041 tttctgccag ggtggtgatc gctgtccgct gagccggcgc cttctgaccg tcctgactct
    11101 tgatccgttt caccacgtct tgaaacggat gactgtccgt gtccgatccg gcaagggcca
    11161 cgatgatcga acgtttggtg tctgtgacgt agtggttaag aaccggaggc agatcacgcc
    11221 gtgctaaccg acgcaggacc atcgcctcgg gatcgagttc gatcgtcagc ggcttgaatg
    11281 gcaagtcact cttgaacagc gtgtccgcac cggacagctg tgcccagagt aggtgctggc
    11341 tgctgttcga catcccgaca agaatcggaa gcggagatcg aaacactggg ccggtttgag
    11401 cgatgctgcc tgatagcgtg aagcgaccgt ccgcatcgcg ggcaagagga tgtgccgtga
    11461 cgttcttcaa ggttaatgca ggcgcgccgg tgccctcgac ccattgcgca aagaaccagc
    11521 ggagatcctt ggcagcggtc tctgcgaaga ttcgctcgac gtcatgccag tcggcatacc
    11581 ggccacgata ctgcgccaca agcgttttta acgagcgcca aaaggacact tcgccgactt
    11641 cctgccgcag caaatgaaag accatcgccg ctttttgata cccgatagcg ttgtcccgct
    11701 catcctgctt ccgggtaaat tgcccaagcg gatagtcacg ttcaggcggc acgtgcaggt
    11761 tatatccccg cagcatcaag tggcgctgat cccgcgcttg ttcatgatcg ccggaccact
    11821 cgtgccaata atagttggca agataggtcg tcaatccttc cacccaattg ccacgatcca
    11881 catggttgaa gaccgagttt ccgatccagg aatgcacgat ttcatgacct aaggcatatg
    11941 gctgcacata gtgtcgtttg atcaccccgc tccccaacag cgtaaacgag ggcataccca
    12001 gcccgctggc aaaaaagttc tcgaccacgg caaacgactc gaacggatag ggaccgagca
    12061 acgacatata cacgtcgaga tagcgagcag tggcatcaag atattcatca gccaactcgg
    12121 cgtcttctgg aaacagatac gtggccaacc ggaccgcctg accgctgctc gacgaccact
    12181 cgcgggtggt ctccacaaat cggttggcga ccagcgtcag cgcctcgctc tcttcgaccg
    12241 tctcccactc ggccagcgtc aggctctcgt cgcgacacag ctctgccggg caagggacgg
    12301 aggaccgacg ctgtccttgg gcgaccgccg tccatcccga gggcagagcc acacgaagcc
    12361 gataccggct gagggattcg gggacatcaa ggtaccaccg ggtttcgctg ctcaggtaaa
    12421 ccccttccat cccgatgtga ccggcggtct cactgggagt cacaaagcgc aaatgacgag
    12481 gctctttggg aggatcgtcg atcgctccat gatagtgcgc ggttacggtc atgacgcccg
    12541 ccgtgacaag ggcggcgggc agtgtgatgt actgcccgaa aggagcagct gcctcgtgct
    12601 cggtctgaac cgggagatcg cgaccactgt cgtcggtggc agatcgggaa agtaccaacc
    12661 gatcaaggtg aagcgtcccg gccagggaca gtcgaagtgg acgtccaggg atggcattga
    12721 tcgtgagacg gtctacggcc agaagcgcat gccgctccgg atcaatctgc acgaacagat
    12781 catggcgcga gatgaaagga gccgcaaagg gctgtggcgt cgccgtcgtc actacagacg
    12841 ggctgagaca gaagaatagc cagagggccg ccatggcggg tcgcaggcac gattttccca
    12901 tgcgttgggt tttagcactc gcgacagacc agcacaagct ggatgatgga gagcaaccta
    12961 tcgagatcga tgggcttact gatcacttct tggggacgca gcgaccacgc gtcttggagc
    13021 aattcttcgt cgtagctccc cgtaacgata atgaccgcgc ccgcgaaatt attggtgcgt
    13081 aggcgacgga gcacctcgac gccgttcatg cccggcagca tcagatccaa taaaatgaga
    13141 tcgggaggag tctgttctat catcgacagc gcctgcgcgc cgtctttgac tcccaaggct
    13201 cgataccccc gcagactcag aaactgcacg agcagatcgc aaaccagctg ctcgtcatcg
    13261 acgatgagca cggtttcccc cgtctctgcg acggcattgc caggttgagc cgtcgctcct
    13321 tccgtctttt tggcaggctg ctgaacgaca cgattgacac cttctaccaa cacatctagc
    13381 gacaaccctt tgcggacaaa atccgtcacg cgcaatgtac gagcctggtt ctcctgaatc
    13441 tccgtcgcac cgccgccgag aatgatgacc ggcgcgtgcg gatcgatggc tcgaatttct
    13501 ttgagaactg tgagcccgtc cattccaggc atccgcagat caagaatcgt gacgcgtgga
    13561 ttttgctggc ggaagaggtt caggccttcc gggccgctgg tcgcggaata cacatcatat
    13621 ccatgacgag tcaagacggc ctgcaggagg tcgcagttca tgcggtcgtc gtcgatgaca
    13681 agaatcttga ccattcccac ccttcaatgc gcaaacggcg tgctacgccg aggcacggac
    13741 cgagcctagc atgcggacta tggcttgaga agtaaaagag atataccggc cttcgagtta
    13801 gtcgatcgcg gcaccaagca aggcatcgac gaaagcctct ggctggaaat cctgcaaatc
    13861 atcgaccgct tctcctacac cgatcaggcg tacaggcaat ttgagctcat ccgcaatcgc
    13921 cacgacgatg cctcccttgg cggtcccatc cagtttggtc agagccaggc ctgtgactcc
    13981 cacggtctga taaaactggc gggcttggga cagcgcgttt tgccccaatg ttgcatctaa
    14041 aaccagtaag acttcatgcg gcgcgcctgg caaggcctgc ccgatcactc ttgacacctt
    14101 ccggagctct tccatcaaat tggacttcgt atgcagacgg ccggccgtat cgatgaggac
    14161 aacatcggcc tgcctagctt tcgccgctgc aatcccgtcg taagccactg ccgccggatc
    14221 cgcgccatgc cgatggcgga tcatctcgac accgatccga tcggcccaga cctgaagttg
    14281 gtcaatcgcg gcggcacgga aggtatcggc cgcgacgagc aacgggacgc gcttgctttg
    14341 gaccagtcgt tgggcgagct tggccatgct ggtggtcttt ccgactccat tgactccgac
    14401 cacgaggacg acaaacggtt tcggtccatt ggcgatcaac tgctcgaacg tgggccccga
    14461 cgctggggtc aaaatttcga gcaaggtctg acgcaatgtc gcatgaacat gcgtggcttg
    14521 cgaggcatcg gtgccctgga gggcctcttt cagacggctg atcacccgct cggccacagc
    14581 ggctcccagg tccgaggtga tgagcgccgc ttcaaaatcg tcgagcaaag cgggatccac
    14641 tgcccgaccc aacaagcgat cgagggaacc gcgcatcacg gagcgcgtct tactcagacc
    14701 ttcactcagc cgctggaacc atcccatgga tgtaactccg cccttatgat gatttccgaa
    14761 cgtaaggacg ccgaccggcg aaacacgcaa tcctgcccag ggcgagccgg accgtccgct
    14821 ctttccgtcg catccggcgg gattcggcct ggccacgctc atggtcatag ccgcataagt
    14881 gaagggctcc gtgtatcaat aaggccacga tttcttcctc caaggagcga cgaccttcct
    14941 tcgcttggcg aagagcagtt ggaacggaga tgacgatgtc tcccaacacc cgtctggaaa
    15001 catgaaagac aggtgttcga gcttctctca acgcaaacgc gagcacgtcg gtgctgcgat
    15061 cttttccacg gaagcggtga ttaagtctcc tcatccgccg atcacccaca aacgacaatc
    15121 cgagatcggc ggaggactcc ccgatcaggt tgagtacggc ctgtgccagt cgcaggatgc
    15181 tgtgacgacc aaaccgtatc gatcgtacat gaacctggag ggaaacgggc attaatgcgg
    15241 ctggccccaa gagcctttcg ttgcgctggg tagggaggag gacgggcgag tgtgggagga
    15301 ctgacggcgc cccccctgcg cggcctgtgg atgggcctgc tccgtctgct cgtgctgatc
    15361 atacgctttg atgatgtctt gtacgagctt atgccgcacg acatcacgct catcaaagta
    15421 cacgaagcga atgccttcca cgtggcgaag aatgtcgcgc acttcgatca accccgaaac
    15481 ccgatcgggc ggaagatcca cctgggtaat gtcaccggtg accacggctt tggagttgaa
    15541 gccaagccgc gtcaggaaca ttttcatctg ttcggccgtc gcgttttggg cttcgtcgag
    15601 aatcacgaag gaatcgttca acgtgcggcc tcgcataaaa gccaatggcg caatctcgat
    15661 gtcccctcgc tcgatggcac gcgttgcccg ctccatatcc atcatgtcga acagggcgtc
    15721 atagagggga cggagatagg gattgacttt ggcgatcatg tctccgggca gaaatccgag
    15781 tttctctccg gcttcgacgg caggcctggc cagaatgatg cggctgactt cacgtttcat
    15841 caacgcgctc acggccatgg ccatcgcaag atacgtcttt cctgttcccg cagggccgat
    15901 gccgatgacg atgtcgtgcg tttcgatcgc ttcgatgtag gttttctgcg tgggcgtctt
    15961 gggagcgacg aagcgttttt tggtgacgat cgtcgtcgca ttggagagca actcttttaa
    16021 cggggtctct ggactttggc gcaaagcggt taatgcatgc gtgacgtcat cgggctggat
    16081 cagcatgccg tcattggcca agcctgccag ttcggtgagt acgcgctccg catagcgcgt
    16141 cgcttctggg tttccgtcaa gggtgagttc ctcgccccgt gccgacaggc gcacgccgag
    16201 gtcttcttcg atcagcttga ggtgccggtc gtggtgtcca aacagaacgg cggtgttggt
    16261 accttctcgc agtttgagct ttcgcacgag gggccgaagc ccgatcctcc tctcagtact
    16321 gtcatgtgat tctaacaaag cggcggcaga gggacaagcg aaaggtcaga agaacagcat
    16381 cccggtgctc ctcggttggt tgaccaccac tggggtagtt gaccatgcat catgggccat
    16441 tgcctgccct ctaaggcagg accctgcgac gtgcgagggc tgtacagccg gtagtggccg
    16501 acacgatccg gtgcgacagg ctacttgctc gccacagctc tgacctgccg gtaggttgtc
    16561 aggccctgca ggactttgcg gataccatcc tgaagcagcg tcagcatccc gtcttcaacg
    16621 gctttgatca gcatagcggc cgttcgactt cttgactgaa tgagttgctt gatttcgtcg
    16681 gttccgacca gcaactcatg tagaggcact cgtcccttga atcctgaatg gcgacagtat
    16741 tcacatccgc gtcctcggaa cagttgaagg tcgtcttgcg cggcatggga taagggccac
    16801 ccttcagcgc cgtaaccctc gaccagttct tgatactcct caggagacgg acgataggat
    16861 tctttgcaga cgccgcagat tcgtttacac aatctggtgg cgagcacacc gagcatggcg
    16921 tcggcaaaat taaacggatc gcacccaagg tcaagcagac ggataacggt ttcgacggca
    16981 ctgttcgtgt gcagggtgct cagaacaagg tgtccggtca gagaagcttc aattgcaatg
    17041 tcggcagttt ctttatctcg catttccccg atcatgatga cgtcagggtc agctcgtaaa
    17101 aacgaacgca tggccgtcgc aaagtccaag ccgattttct ggtgaatctg cagttgccgc
    17161 aacccttctt gcgtgatctc gatcgggtct tcagcggtcc aaattttgcg ttcttccgtg
    17221 ttgatgtgct tcagaatagc atggagcaac gtcgttttcc ccgagccggt cggccccacg
    17281 cacaggatga tgccgtgcgg cttttccgcc atcgccttga ccgtctgcaa tgcgctcggg
    17341 gtcaactcca tagcttccaa aggcatcggt tccttcgcgg tcaaaaggcg cagcacggca
    17401 tcttcattgc caccggctgt tggcagggtg gccacacgca actcaaggct gcgttgggcg
    17461 tcaagcttga atcgaatctt cccatcttgc ggcttgcgcc gttccgcaat gtcgagattc
    17521 gccataatct tgaggcggga gatcagtgcc cggcggtaag cggttgggac tttcgtgtaa
    17581 ctaaagcacg tcccgtcgac ccggaaccgg atgttcatct ctctcttgct cgtgctcggc
    17641 tcaaaatgca gatcggaagc gccaagacga tccgcttcgc taatcaaatg gttggtgagc
    17701 cgtacgataa gagaatcgtt ttcgtcgata gggccggaag gcctctcgag caccggatca
    17761 agaggaagat cttcctccaa ttcacttaat atggttccga tggttgaagc gctcccctgc
    17821 tgctccatcg tggcgccaag aaactgttca atgtcacggc gaagcccgac ggaataccga
    17881 atggtcatgc caggaaaact
//
LOCUS       DASTQY010000351         6380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_473312, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000351 DASTQY010000000
VERSION     DASTQY010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473312"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /gene="dnaA"
                     /locus_tag="VFR82_11550"
     CDS             complement(<1..1155)
                     /gene="dnaA"
                     /locus_tag="VFR82_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU4502276.1"
                     /translation="MGESAGTMTVSTVWQEALAYIQGRVPKQVYDTWFMPVRLERIED
                     RNAQIGVPNKFFGDWLSQHYGPLLEEAVSTARGGGETAVSFVVFSRPPISQSDPAAVA
                     QAVRTATTPKSKRGIQLNPKYSFKNFVVGAGNQFAHAACMAVAEQPAKAYNPLFIYGG
                     VGLGKTHLLNAIANHVAERTDLRIAYLTTEQFTNEVINSIRYDKMMDLRKRYRHIDML
                     MIDDIQFLAGKERTQEEFFHTFNALYEAHKQIVLSSDRFPKDMPDIEERLRSRFEWGL
                     IADLQPPDVETRIAILRKKSEDEGVTLPEDVIQFLSTTMKSNIRELEGSLVRLGAYAS
                     LTGQSITLDMARNVLRDLIGDKKKIVAMEDIQEAVAACFHIKIADLKSRRR"
     gene            complement(1466..1696)
                     /locus_tag="VFR82_11555"
     CDS             complement(1466..1696)
                     /locus_tag="VFR82_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502277.1"
                     /translation="MKNTLYLLRKPVDRVDPALFIASHSKGDVVLLDTALVSFPHSSG
                     TVFSLGTQDGERTISYDVLVQKIFESDAAIVI"
     gene            complement(1693..2037)
                     /locus_tag="VFR82_11560"
     CDS             complement(1693..2037)
                     /locus_tag="VFR82_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502278.1"
                     /translation="MSKRVAVVIAEDPTKTHRPVEALRIALGLCAGDHETTIILLGNA
                     PLLVTDDTDDVVDVDILEKYRPSFKHLHVPFVVASGSRTQKFLDGFSVTSKPPDEIRT
                     LVQSLDRTLVFS"
     gene            complement(2052..2372)
                     /locus_tag="VFR82_11565"
     CDS             complement(2052..2372)
                     /locus_tag="VFR82_11565"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEU4502279.1"
                     /translation="MAQQKLGLLLSTAPSHPSVETVVRLAEAALRQGVEVYLYLIDEG
                     VKSYTDTRFRGLLDAGVRMSVCAYGCQQHGVSTEQVDPRVSLSGLVVLSGIIDGCDRF
                     LAFT"
     gene            complement(2486..3742)
                     /gene="hisS"
                     /locus_tag="VFR82_11570"
     CDS             complement(2486..3742)
                     /gene="hisS"
                     /locus_tag="VFR82_11570"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU4502280.1"
                     /translation="MIKGIKGVKDILPEETPRWRFIEETARQWADRYGFHEIRIPIFE
                     VTTLFARSIGATTDIVEKEMYTFPDRDGTSLTLRPEGTAGTVRAFIEHHREADPLPQK
                     YFYIGPMFRHERPQAGRLRQFHQFGVEYLGTQDPKADVDVIALLWRFLSDLRLPDLIL
                     EVNSLGTNTDRAAYLPVLVAFLQSRVGQLCENCRRRIDTNPLRVLDCKVPACRAATED
                     APHLTSYLSPEAKDHFDQVLSTLSALGIAFRLNPRLVRGLDYYCLTAFEITSSHLGAQ
                     NAVGAGGRYDGLVQLLGGQPTPAVGFAAGLERIAMMLPAERVPTVRPSIYVASFGTEG
                     SPAGVRLLDALRQVGLSALSDYRTTTLKSHLRQADRAGCRYAVLLGDDEARAGSAVVR
                     DMESKAQETIPLTDLPRYFVALSKTF"
     gene            3899..5386
                     /locus_tag="VFR82_11575"
     CDS             3899..5386
                     /locus_tag="VFR82_11575"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEU4502281.1"
                     /translation="MSSDRPTRRVIAVAPMPPEKSAYALARYSRSPDSIEQSIKWVHG
                     HSSEKFWEQFYFDYGHASIADLGHVIICFEEITELAAIRLEDEPLWDGQAKSSRYQNF
                     ASGGWYVPDAIHGSETEGTYHGILRSLGEIYRLLHEPLVTYLSEREPRPPSMKPETYQ
                     RTIAARAFDVTRYLLPLACKTNVGQVVSIRTLEKQITRLLSSQLPELQMIGEDLKDAC
                     QRSPMNVWGELCGQTAGLNEPMAPTLSRYARANDYQATVYTDLARYAKEALRGTGLDH
                     ADTWGTCESVELIDPHDPIDETVATLLYRVSHAPYRSILGVVRDWTDKQKQDTIAIAL
                     SRRGPYDELPKEFRSGYAFMFDVLMDIGGWRDMHRHRRCQQIQQNFTTAHGYDVPAPL
                     VEARLEGEYRQAMDAVRADIEQLKKVNGEAALYAIPFGFKVRCLFKMDFAEAEYIARL
                     RSGVKGHWSYRNIAWLMKQKLTERYPMLGVHVQATSPDIEDTLTR"
     gene            5403..>6380
                     /locus_tag="VFR82_11580"
     CDS             5403..>6380
                     /locus_tag="VFR82_11580"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4502282.1"
                     /translation="MTEHQINCEAALLDGDWEQAAREALAWARHPKSTDRRDPRPHFA
                     LNVVQLIKGQFAEAWSSHALSLQEPDDIAQVKSWLDGLVASHADRPSAHLVMGLFLSQ
                     SGQSEQSMQSYRTATKLAPQSAYPHYFLAQIHERANHVEMAIKEYREAVRLDPDYVPA
                     RTNLGVAYQEQGRLEMAIPQYREVIKLNPSDAVAHANLACALAEQGKMQAALLEYQEA
                     LRLNPRDAEVHFALGGLYENKNRPDLAMKEYRAAIESNRDLAPAHSALGWMLFDRGHR
                     EEAMECFNQALRADPQNARAYLGVAKCYASRGKVQLAIENYTKAIKYEQD"
BASE COUNT         1438 a         1777 c         1805 g         1360 t
ORIGIN      
        1 cctgcgccgc gacttgagat cggcgatctt aatgtggaag caggcggcga ccgcttcctg
       61 aatgtcctcc atggccacaa ttttcttctt gtcgcctatc aaatcacgca gcacgttcct
      121 cgccatatcg agagtaatgg actgtccggt caacgaggca tacgcgccga ggcgcaccag
      181 cgagccttcc agttctcgaa tgttgctctt catggttgtg gataagaact ggatgacgtc
      241 ttccggcagg gtgactcctt cgtcttcgga cttctttcgt aggatcgcga ttctggtttc
      301 cacatctggg ggctgtagat cggcgataag cccccactca aaccgcgacc gcaaccgttc
      361 ttcgatgtcg ggcatgtctt tcggaaaccg gtcgcttgaa aggacgatct gcttgtgtgc
      421 ctcatacaag gcattgaagg tatggaaaaa ctcctcctgt gttcgctcct tgcccgctaa
      481 gaattgaatg tcgtcgatca tcaacatgtc gatatgccga tatcgcttcc gcaggtccat
      541 catcttgtcg tagcggatgg agttaatgac ctcattcgtg aactgctcag tcgtcagata
      601 ggcgatgcgc agatctgtgc gctcagccac atggttggcg atcgcgttca acagatgggt
      661 cttaccgagg ccgactccgc catagataaa gaggggattg taggctttgg ctggctgttc
      721 tgcgaccgcc atacacgcgg cgtgcgcgaa ttgattgccg gctcccacga caaaattctt
      781 aaaagaatac tttgggttaa gctggattcc tcgcttggat ttaggggtcg tagcggtccg
      841 gaccgcttgc gccaccgctg cagggtcgct ctggctgatg ggaggtcggc taaagacgac
      901 aaaggacacg gccgtctctc cccctccgcg ggccgtcgac accgcctcct caaggagcgg
      961 tccataatgc tggctgagcc agtcaccaaa aaacttattc ggaacgccta tttgcgcgtt
     1021 tcgatcctcg attcgctcta aacggaccgg cataaaccag gtgtcatata cctgttttgg
     1081 aacccgtccc tgaatgtaag ctaaggcttc ctgccaaact gtagaaactg tcatagttcc
     1141 tgcgctctca cccatacaca ggcttattca cacctgtgga taagtatgct cgatgccttc
     1201 gcttctccgg acctcagcga tgtcatcagc cagtggtgga ttttactttc tctcttcatc
     1261 gacctctata aagaagttat gcacagtgca ccgtcctctc actccgagtc aatccccagg
     1321 tccgtcccca aggcgtctca cagtaaagtg cttccctaat caacccgtta tcaccaatcc
     1381 ccaccatcca ccggtcctac tccgaatcct acgacggcga ctgttttctt tctttggcac
     1441 atcatcttcg gagaagagcc ctttcctaaa tcacgatcgc cgcatcactc tcgaaaattt
     1501 tttgcacaag gacgtcatag gagatggtgc gctccccatc ctgagtgccc aatgagaaga
     1561 ctgttccgct ggaatgagga aatgaaacca gggcagtatc aaggagcacc acatctcctt
     1621 tcgagtgtga cgcgataaac aatgcaggat ccacacgatc cacgggtttt ctaaggagat
     1681 aaagcgtgtt cttcatgaaa agaccagggt gcggtcgagt gattgcacaa gcgtgcgaat
     1741 ctcgtccggc ggtttactcg tcacggaaaa tccgtcgaga aacttctgcg ttcgcgagcc
     1801 tgatgctacg acgaacggca cgtggagatg cttgaacgag ggacgatact tttcaagaat
     1861 atctacgtcg acgacgtcat cggtatcatc ggtcaccagg agaggagcat ttccgagaag
     1921 aatgatcgtc gtctcatggt ccccggcaca aaggccaagt gcaatgcgga gggcctcgac
     1981 gggccgatgg gttttagtag ggtcttcagc gatgacgacg gcaactcgtt tggacatgtc
     2041 gcccgttctt gttaggtgaa tgcgagaaac cgatcacatc cgtcaattat tcctgagagc
     2101 acgacaagcc cagatagcga gacacgcgga tcaacttgtt ccgtcgaaac gccatgctgt
     2161 tggcatccat aggcacagac ggacattctg acgccagcgt caaggaggcc tctgaagcgg
     2221 gtatccgtgt agctttttac gccctcatca atcagataca aatagacctc aaccccttgg
     2281 cgcaatgcag cttctgccag tcgcactacc gtctcgacgc tcggatgaga gggggctgtg
     2341 gataacagga gaccgagttt ctgttgtgcc atggaaatat ttgtgaattt aacacgaaga
     2401 tcttttaaga atcaattggt tatataaacc agtggtgccc gagactacga ggttttcgag
     2461 aggaagtcaa ttggaaaaaa gggtctcaaa aagtcttgct gagggccacg aaatagcgag
     2521 gaagatcggt caatggaatc gtctcctgcg ccttggattc catatcccgc acgacagcgg
     2581 atccagctct ggcttcatca tcacctaaga ggacggcata ccggcagcct gcacggtctg
     2641 cctgacgaag atgggatttt aatgtggtgg tccggtagtc ggacaaggcc gagaggccga
     2701 cttggcggag ggcatccagt aagcgcacgc ctgctggact gccttcggta ccgaacgagg
     2761 ccacatagat cgagggacga accgtcggaa cacgttcggc agggagcatc attgcgatgc
     2821 gttcgaggcc ggccgcgaat cccactgcag gcgtaggttg cccgcctaag agttgaacca
     2881 atccatcgta ccggcctcct gctcccacgg cattctgcgc tccaaggtgg gaggacgtaa
     2941 tctcgaaggc cgtcaggcaa tagtaatcga gtccgcgtac gagccgtgga ttcagacgga
     3001 acgcgatccc gagtgcggaa agtgtcgata ggacctgatc gaaatggtcc tttgcctcgg
     3061 gagacaagta tgaagtcagg tgaggggcat cctcggtcgc tgcccggcag gcaggcacct
     3121 tacagtcgag gacacgaagt gggttcgtgt cgatccgtcg ccggcaattc tcgcacagtt
     3181 ggcccacgcg gctctgcaaa aacgcgacga ggaccggaag gtaggccgct cgatcggtat
     3241 tcgttcccag gctgttgacc tcaagaataa gatcaggcaa acgcaaatct gagagaaatc
     3301 gccatagcag ggcgatgaca tccacgtccg ccttaggatc ctgggttccc aggtattcga
     3361 cgccgaactg atgaaattga cggagacgtc ctgcttgcgg ccgttcatgg cgaaacatgg
     3421 ggccgatgta aaaatatttt tgcgggagag gatcggcctc tcggtgatgt tcgatgaagg
     3481 cacggacggt tcccgcggtt ccttccggtc tgagcgtgag agacgttccg tcccggtcgg
     3541 ggaatgtgta catttccttt tccacgatgt cggtggtggc gccgatgctg cgggcaaaca
     3601 gcgtggtgac ttcaaaaatc gggatgcgga tttcgtgaaa gccataccga tccgcccact
     3661 gtcgggcggt ctcctcgata aaccgccagc gcggcgtctc ctcagggaga atatctttga
     3721 cgcccttgat gcctttgatc acctgtgcat ccagctttcg tgatcgcgtt cctaaaggtg
     3781 cgcgcgacta tagcgacgag tttgcgggca agtcaacgcg acgagggctt cttgcgccca
     3841 gcttcgagcg gcgctatagt tcgcaacctc ccggcgtcct taaaacagga gtctgtgtat
     3901 gagcagtgat cggccaaccc ggagggtcat cgccgtggcg cccatgccgc ccgagaaatc
     3961 cgcgtatgcg ctggcgcgct acagtcgctc tccggactcg atcgaacaga gcatcaaatg
     4021 ggtgcacggg cattcctctg aaaagttctg ggagcagttt tacttcgact acgggcacgc
     4081 ctcgatcgcc gatctcggcc acgtcatcat ctgttttgaa gagatcaccg agcttgccgc
     4141 catccggttg gaagatgaac cgctgtggga cgggcaggcc aagtcaagcc gctaccaaaa
     4201 ctttgcctcc ggcggctggt acgtgcctga cgccattcat ggaagcgaga ccgaaggcac
     4261 ctatcacggc attcttcgca gcctggggga aatctaccgc ctccttcacg aacccctcgt
     4321 gacctacctg tccgagcgag aaccgcggcc gccctcgatg aagccggaaa cgtatcagcg
     4381 gacgattgcc gctcgcgcat tcgacgtcac gcgctacctc ctgccgctcg cgtgcaaaac
     4441 gaatgtcggt caggtggtca gcattcgaac gctcgaaaag cagatcacga ggcttctttc
     4501 ctctcagctc ccggaactcc agatgatcgg ggaagacctt aaggatgctt gtcaacgttc
     4561 cccgatgaac gtctggggcg agctgtgcgg gcaaaccgcc ggcctgaatg aaccaatggc
     4621 tcccacgctc tctcgttacg cccgggcgaa cgactatcag gccaccgtct acacggatct
     4681 ggcccgctac gcgaaggaag cactccgcgg cacggggctc gaccatgcgg atacctgggg
     4741 aacctgcgag tccgtcgagc ttatcgatcc gcacgatccc atcgatgaaa cggtcgcgac
     4801 gctgctgtat cgtgtgagtc acgcgcccta ccgcagtatc ctcggtgtcg tgcgtgattg
     4861 gacggacaag caaaaacagg acacgatcgc catcgcactg agccgtcgcg ggccgtacga
     4921 tgaacttccg aaggagttcc gaagcgggta tgcattcatg ttcgatgtcc tcatggatat
     4981 cggcggctgg agggatatgc accgtcatcg gcgctgccag cagatccaac agaatttcac
     5041 cactgctcac gggtacgatg tcccggcgcc cctggtcgag gcgcgtcttg agggtgaata
     5101 tcggcaggcg atggatgcag tgagggcgga tattgagcag ctgaagaaag tgaacggaga
     5161 ggcggccctc tatgccatcc ccttcggatt caaggttcgc tgtctgttca agatggattt
     5221 tgcagaagcg gagtatattg ccaggcttcg ttcgggggtc aaagggcact ggtcctatcg
     5281 gaacattgcc tggctgatga aacagaaact caccgagcgc tatcccatgc tcggcgtgca
     5341 cgtacaagcg acttcgccgg atattgaaga tacgctgacc agatagcgcg tttccccctt
     5401 ccatgaccga gcaccaaatc aactgtgaag ccgccctgtt ggatggagac tgggaacagg
     5461 cagcgcggga ggcgcttgct tgggcgcgcc atccgaagtc gacggatcgg cgggatcctc
     5521 gaccacattt cgcgctcaac gtggtccagt tgatcaaagg gcaatttgct gaagcctgga
     5581 gcagccacgc tctctcgctc caggagccgg acgacattgc gcaagtgaaa agctggctcg
     5641 acggactcgt ggccagccat gccgatcgac ccagtgctca tctggtgatg ggattgttcc
     5701 tgtctcaatc cgggcaatct gaacaatcga tgcaatcata ccgaacggcg acgaagctgg
     5761 cgccgcaatc agcctacccg cattatttcc ttgcgcagat tcatgaacgc gcaaaccacg
     5821 tcgagatggc aatcaaagaa taccgggagg cggtgagact cgatccggac tatgttccgg
     5881 ccagaaccaa cctcggggtg gcgtaccagg agcaaggacg attggagatg gccatcccgc
     5941 aatatcgcga agtgatcaag ctcaacccga gcgatgccgt ggcccatgcc aatctggcct
     6001 gcgcgcttgc cgaacagggg aagatgcagg cggcgctctt ggaatatcag gaggcactcc
     6061 gattgaaccc tcgggatgcg gaagtgcatt ttgcgctggg agggttgtat gaaaacaaga
     6121 accgtcccga tctcgcgatg aaagaatatc gggccgcaat cgagtcgaat cgcgacctgg
     6181 cgcccgctca ttcggctctc ggctggatgc tgttcgatcg gggtcatcgg gaagaggcca
     6241 tggaatgttt caatcaggcc cttcgtgctg atccgcagaa tgcgcgagcc tatctggggg
     6301 tggcgaagtg ctatgcaagc cgaggaaaag tgcagctggc gattgagaac tacacgaagg
     6361 cgatcaagta cgaacaggac
//
LOCUS       DASTQY010000352         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_970584, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000352 DASTQY010000000
VERSION     DASTQY010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970584"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..725
                     /locus_tag="VFR82_11585"
     CDS             162..725
                     /locus_tag="VFR82_11585"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HEU4502283.1"
                     /translation="MRYATANARWEKPMGGLTNVIACCALVSACSPSVFPYKALDGVN
                     HDFDFLGWQLLQDQNPQQKVQLGGRIVQAEMKDDMVTITATHLSIPKDPTQEPEEAKP
                     SGEFLIQYSAVIDPLVLRSGNKLIVVGETGSPGQIEVEKKVRLLPRITALCIHFWNTD
                     GKGIALRGSSSSGTKFLAELTYCKTAL"
     gene            complement(2116..2382)
                     /locus_tag="VFR82_11590"
     CDS             complement(2116..2382)
                     /locus_tag="VFR82_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502284.1"
                     /translation="MARRKRVRTATVEWNGNDWRRAWKIALTRIHGAPAVVMEQMAFH
                     HAVDMLDCGFARGDAFQFQLGMLMMIDCCNEAILRGDCYQWWID"
BASE COUNT          733 a          722 c          772 g          726 t
ORIGIN      
        1 tggcccaggg ctactatgtt ccggcttatg cccgacgtga cagcttcacc tgtttgattc
       61 atgaggacat ttgaggacgc ggtctggtct tatattagct ttgcaataca aatgacttat
      121 gagctattca tttggcggga aggctcagtg gagcttcttg catgagatat gcgactgcaa
      181 acgccagatg ggaaaaaccg atgggcgggc tcaccaacgt cattgcatgt tgtgccctag
      241 tcagcgcctg ttcaccctcc gtcttccctt acaaagcgtt ggatggtgtg aatcacgact
      301 ttgacttttt gggttggcaa ttgcttcagg atcaaaatcc gcagcagaag gtgcaattgg
      361 gagggcgtat cgttcaggct gaaatgaagg atgatatggt caccatcaca gcaactcacc
      421 tgtcgatacc caaggatccg acgcaagagc cggaggaggc caaaccaagc ggtgagttcc
      481 tcatccagta tagcgctgtt attgatcccc tcgttcttcg ttcgggaaac aagctcattg
      541 tagtaggcga gaccggcagt ccaggacaga tcgaggtcga aaagaaagtt cgcctcttac
      601 caaggataac cgccttgtgt atccattttt ggaacaccga tggcaaaggg attgcgttgc
      661 gaggatcttc aagttctggc acgaagtttc tggctgaact gacctactgc aagacagcac
      721 tgtgagtgcc tgcatgcgac atcaaaaggt ttcaggacgc ttctgttgaa gcgctcatat
      781 caggcttaaa ggtgtcatga gagcccgact taccaagcaa gccatgaatt agagctgggc
      841 tctggaaaag cgataccgct gtcacattac atcattggcc gttcgcagaa accacctcga
      901 cagcattccc attcacgctt cgctcgtagc cgacttcgcc tcccggggtc atttctcata
      961 gttgaaccgc ctctattctt ctcgaataag ccacttttaa gctcgcttta atctgtacct
     1021 aatttcactg atttatttgt aagcgttgga ggaggccaat gagttggcga gcctattgtt
     1081 cagcgctggg aatggcgatc gcatcgctct ttgcgagaag cggagttttt cagcggccgc
     1141 tggaaaggaa gtagacgctc tcgacgggct acaacagctc ggaggaactc atgtcgcagc
     1201 ggattaagac taactacagc tacaaagggt ggaatataac cttgataatg aaccttgaag
     1261 gttacatgtg gacctgtcag tacataatca ggaagtcggg caggacggtg atggatggat
     1321 ttccaggaag cacttatctt tcgcgcgaag aagcggaaga gacggctttg aggactgcca
     1381 aaagcctgat cgatcaatgt aagctggaca agaatccact aggtacttaa gtcagttggt
     1441 gcgtcactgc gaattgaagt cggcccctct cgtgactcag ctgtggacag gtaggcacaa
     1501 cgaacatcac gcccgcctct cagacgaatg aacctctctt ctcttgtgag tactcaggag
     1561 tttcgccatt tgttccgtgt agaactcaag taatcggtgt tcttccttgg aaaacggtgg
     1621 gctgttgggc accgaagcaa cggagatgag atattcacag gagcgcacgt agttctctac
     1681 gtctttgcgg ggggctttct ggggtgggcg gagggacatc ggattctcac ctgatcatcg
     1741 attactccag agccgttgac acctgcaaat tactctagga ggttactgaa tagtgtgtca
     1801 acaggaatac tgtcggcatc aatccgtcag ctatttccta cattcttggc ggtgtctagc
     1861 ggccgcttca caaagtattg ttgaggatga ccgcacactc gctcgtgcgg tggatttccc
     1921 aaagcggtgg gagtatggtg aactaaatgt ggcaaagact gttgcaagcg acggccgtca
     1981 cgaccttgtt attttttctc aagcgcttgt tcatccggaa taaatcccca ttaccctacc
     2041 gccaccctgt ctctcggtgg ccgagtcgtt cttaccgcaa accataggga cacggcgggg
     2101 aggagcccgt atgggtcaat cgatccacca ctggtaacag tcgccccgga gaatcgcttc
     2161 attacaacaa tcgatcatca tgagcatgcc gagctgaaac tgaaacgcat cccctcgggc
     2221 aaagccacag tccagcatgt ccaccgcatg atggaaagcc atttgttcca ttactacggc
     2281 aggagcgccg tggatacgcg tgagggctat tttccatgct cgtcgccaat catttccgtt
     2341 ccactcgaca gtggccgtgc gtacccgttt ccgacgcgcc atctcccctc cgagtcacaa
     2401 aactaacata ctcagcagaa aggcttgtta gtggctgctc taggagaggt aaacttgtgc
     2461 agttgatcac gtaaagattc atgctagtgt cgaggtggcg acttgaagcc agttccgccc
     2521 caacgaaatg agagttacgg tattccacgg tatgctgaaa atggcgagtt tgttggatac
     2581 atcggttcgt gtatcgatgt cactgaacgc agaaaagcgg aggagacagt ccaagcaatg
     2641 gcattgctgc cggttcaaaa cccatcaccg gtcctgcgca tagatggaga tggcattctc
     2701 ctttataaaa atcccgcagt ggatcagtca ctccgagatt cggctttaac tgttggtagc
     2761 cccgtgtcgc cacatctgat ggccttagtt caaggtcatt acatgcacgt cttgcacaaa
     2821 tggtggaagt acatatgcat gggcgagtgt atctagtaaa cgtgaccccg atcgagaagg
     2881 ataattatgc aaacctatat tggacggata tcacagattg taaatgggcc gaaaatggga
     2941 cttagattta ggg
//
LOCUS       DASTQY010000353         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_698384, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000353 DASTQY010000000
VERSION     DASTQY010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698384"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..783)
                     /locus_tag="VFR82_11595"
     CDS             complement(361..783)
                     /locus_tag="VFR82_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502285.1"
                     /translation="METGNVLDAIGGLTSQLKTYAATLPAIVHLTVVPTGIKPKPEAV
                     DDYEVIVSRFRSQSAGTAYKGLNDAFVESLEAFEVGKLLGSVQPLLAVLDHLDRMQRD
                     GEILVEQGNEKRITEYRASLHKILPGNKPELDGAGRGL"
     gene            complement(793..1779)
                     /locus_tag="VFR82_11600"
     CDS             complement(793..1779)
                     /locus_tag="VFR82_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU4502286.1"
                     /translation="MITEFVVTAGEQPKRLDVFLVNRERDMSRSALQRLIELGRIRLN
                     DHIVRPSQKIKPGDKITMDVPKPEPLQLRGEPIPLEVLFEDEQVLVLNKPAGIVVHPA
                     PGNWTGTLVNALLHYFQTSGGTMSAVGGKERPGLVHRLDKDTSGVMVIAKTDQAHRHL
                     SSQFKQHTITRVYEALVWAIPKKGHGLIELAIGRDSKERKKFSSRTTRPKDAVTGYTV
                     SRRFGKAAAHLLLYPQTGRTHQLRVHLASLGHPILGDPTYGGRKVCCVNGLEVPRVML
                     HARTLGFLHPSTGELQLYERPSPPDMDFLMQQMEERLDIRPARILTEVKRLD"
     gene            1900..2478
                     /locus_tag="VFR82_11605"
     CDS             1900..2478
                     /locus_tag="VFR82_11605"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEU4502287.1"
                     /translation="MRWVLALGAMMLTAGYFLTASKAADQPGIAPETVADYLHSIIEA
                     DRTFYSIHVVERLQKGGGTKAAEDWRAKKNVLPLPAQFLIEASELAAKTGSKVRYRLI
                     SLWPINPKNAPDGPLERQGLEALREHPERSVTGTVKRGDTTYFQAMYADRAVSKTCIT
                     CHNGHRKSPKKDFTLHDVMGGLLSEIPLERAQ"
BASE COUNT          584 a          716 c          730 g          578 t
ORIGIN      
        1 caagttcttt cttctcggag aatgtaacag cggtcttgag ggcggcgggc atcttatact
       61 agagtatcct cgcaactcag gggatgatgg gccttagtgt acgaggaatg cgatggcaac
      121 gcaagtccgt agctgcccca aatgcttcca actcatgtgg ctcacacaga tccagtacga
      181 actgcttgac cacgaaaccg ttcgaacgaa gtgcccgcat tgcggagcga ccgttcgatt
      241 caagttggtg tcggaaggcg caaacgccac gggcccgaaa atgggacatt gatcggacat
      301 cgtacgccgg tcgtactgtc ctctgatgag gacggctcag gacgcaactt cgaccgcagg
      361 ttataaccca cgtcccgcac catccaattc aggcttgttc cccggcaaaa ttttgtggag
      421 gctggcgcga tactcagtga tgcgcttttc gttcccctgc tcgaccagga tctccccgtc
      481 tctctgcatc cggtccaggt gatcgaggac cgcaagtaac ggctggacgg aacccagaag
      541 ctttccgact tcaaaggctt ccaatgattc aacgaatgcg tcgttcagcc ctttatacgc
      601 cgttcccgcg ctttgactgc ggaaccggga gacgatcacc tcatagtcat cgaccgcttc
      661 tggcttgggc ttgatacctg ttggaacgac cgtcaaatgc acgatggcag gcaatgtggc
      721 cgcgtaggtc tttaactgcg acgtgagccc gccgatcgca tcaagaacat tgcctgtttc
      781 cataccatcg tcctaatcga gtctcttcac ttctgtcaag attcgggctg gcctgatatc
      841 gagacgttct tccatctgct gcataaggaa gtccatgtca ggcggggagg gtcgttcata
      901 cagctggagc tcgccggtcg aaggatgaag aaaccccaat gttctggcat gaagcatcac
      961 gcgaggcacc tccaatccat ttacacaaca aacctttcgg cctccgtagg ttggatcgcc
     1021 gagaatggga tggccgagcg aggcgaggtg cacgcgcaat tgatgcgtcc gtcctgtttg
     1081 aggatacaac agcagatggg cagcggcctt gccaaaacgc cggctcaccg tgtagccggt
     1141 gaccgcgtcc ttgggccgag tcgtgcggga ggaaaacttt ttgcgttcct tggaatcacg
     1201 tccaattgcc agctcaatga gaccatgtcc cttcttggga atcgcccaga ccagcgcttc
     1261 gtagacgcga gtaatcgtat gttgcttgaa ttggctagaa agatgccggt gcgcctgatc
     1321 ggttttggca ataaccatca caccggaggt gtccttatcc agtcgatgaa cgagaccagg
     1381 ccgctccttt ccgccgaccg cggacatggt gccgcctgaa gtctggaaat agtgaagaag
     1441 ggcgttgaca agtgtgcctg tccagttgcc tggcgccgga tgcacaacga ttcccgccgg
     1501 tttgttgagg accagaacct gttcgtcctc gaagaggact tcgagcggaa tgggttcacc
     1561 ccgcaattgg agcggttcgg gtttcgggac gtccatcgtg atcttatcgc cgggcttgat
     1621 cttttggctg ggtctcacga tatgatcgtt gaggcgtatc cgtccgagct cgatgaggcg
     1681 ttgtaaggca gaccgagaca tgtctcgctc acgattgacc aggaagacgt ccagccgctt
     1741 tggctgttca ccggctgtga ctacaaactc ggtaatcatg tgtggggcac acgctactaa
     1801 agtgccatcc tccattgcaa tcaggtctgt ttgcctcacg ctggccgcag gactatgcta
     1861 cagagaaagc cgtcttgaac ttgaaaggag attggaatca tgagatgggt ccttgcctta
     1921 ggcgcgatga tgctgaccgc cggctatttc ttgaccgctt ccaaagcggc cgatcagccg
     1981 ggcattgccc ctgaaaccgt tgccgactat ctgcattcga tcattgaagc tgaccggacc
     2041 ttttattcca ttcacgtcgt cgagcgcttg cagaaaggag gcgggacaaa agcggctgaa
     2101 gattggcgtg cgaagaagaa tgtgcttccg cttccagccc aattcctgat tgaggcgagc
     2161 gaactcgcag ccaagaccgg ttcgaaagtc cgatatcgtc tgatcagcct gtggccgatc
     2221 aatccaaaga atgcacccga cggtccattg gaaaggcaag gtctcgaggc cttacgagag
     2281 catccggaac ggtcggtcac aggaaccgtc aagcggggag acaccaccta ttttcaagcc
     2341 atgtatgccg atcgtgcggt gagcaaaacc tgcatcacct gccataatgg gcatcgaaag
     2401 agtcccaaga aagacttcac actgcacgat gtcatggggg gattactcag cgagatccct
     2461 ttggaacgag cccagtagac ggcgaagact ggcggatggc atgcaccaac acctgttgcc
     2521 tgcgataaat tgaaagtgcc tgctcttggg cttctcccgc ctcctgttga tgttggagcg
     2581 tccgatggag ggaggccaat aacgccca
//
LOCUS       DASTQY010000354         3447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_485687, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000354 DASTQY010000000
VERSION     DASTQY010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485687"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /gene="pgi"
                     /locus_tag="VFR82_11610"
     CDS             complement(<1..1037)
                     /gene="pgi"
                     /locus_tag="VFR82_11610"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU4502288.1"
                     /translation="MTTKTRKAPSTMARIKPLPRRKSWKALQAHYKAVRDVHLRTLFA
                     EDPQRGTRLTAEAVGLLLDYSKNRITDQTLELLLQLADESGLRERIEAMFRGEKINVT
                     EQRAVLHVALRAPREASIMVDGENVVPKVHEVLDQMTEFCRRIRNGTWKGHTGKAIRN
                     VVNIGIGGSDLGPVMAYEALKHYSERTMTFRFVSNVDGTDFSEAVHDLQAEETLFIIS
                     SKTFTTLETMTNANSAREWSLARLGGDPKSVAKHFVAVSTNAAEVAKFGIDTANMFQF
                     WDWVGGRYSMESAIGLSTMLAIGPEQFRAMLDGAHQMDEHFRRTPFAQNLPVLMGLLT
                     IWYVNFFGVQTV"
     gene            complement(1071..>3447)
                     /locus_tag="VFR82_11615"
     CDS             complement(1071..>3447)
                     /locus_tag="VFR82_11615"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEU4502289.1"
                     /translation="TFDNVVDVKQASVREQFEAAAHAVRDVVSQRWLQTERTYVEKNP
                     KRVYYLSMEFLLGRSFANNITNALLRPLAMERQKQLGLDPLAIQEMEPDAGLGNGGLG
                     RLAACFIDSMATMEIPGMGYGLRYEYGIFKQTIKDGWQCEQPDNWLRRPDPWEVARLE
                     EAVEVKLNCSFELRSGILRPIVGRPSVLLGIPFDRPVVGYGGKTINTLRLWAAGTPDY
                     FDFQLFSRGDFVGALAETLTAESLTRVLYPDDSTSMGQGLRFLQEYFLVACSLADLVR
                     RFRRGNPDWNALPEKAAIQLNDTHPTMAVPELMRILLDEAHLGWDHAWDLTQRTLAYT
                     NHTLLPEALEKWPFEWFEAMLPRQLEIILEINRRLIEVVRNRFPGDEGRVARVSLVEE
                     GARRKIRMANLAIVGSHSTNGVAAIHSKLLRTVTVKDLAELYPERFNNKTNGVTPRRW
                     LLMANPALSHAITDAIGDGWITDLGELGKLKRLANDRSFQDAFRKAKREAKLQFAQWL
                     KVTSGQSIDPNSIFDCQVKRIHEYKRQLLNALRVVVLYNRLRENSNMVMAPRTFFFAG
                     KAAPAYHLAKVIIKFLNNLAGTIDGDPIVRGRIKVVFLPEYCVSLAERLIPATDVSNQ
                     ISTAGYEASGTSNMKFMMNGALTLGTRDGATIEMAEEAGEENFFLFGLTAEQVASSRD
                     WYNPRWHYEQEPETRAALDLIFSNYFSRNEPGVFAALEQAVLTSDHYMHLADLTSYMK
                     ADRALCDLYADASGWAQKAILNVAGSGKFSSDRTIAEYAAGIWEVKPCPVP"
BASE COUNT          717 a         1039 c          931 g          760 t
ORIGIN      
        1 acggtctgca ctccaaagaa gttcacgtac cagatggtca gcaggcccat cagcaccggg
       61 aggttctgcg caaacggggt tcgccggaag tgctcgtcca tctgatgagc gccgtcaagc
      121 atggcacgga actgctccgg cccgatggcc aacattgtcg ataaaccaat ggcagactcc
      181 attgaatagc ggccgccgac ccagtcccag aactggaaca tattggccgt atcaatgccg
      241 aacttcgcca cttccgccgc attcgtggac acggcgacaa agtgcttcgc gactgatttg
      301 ggatcgccac ctaaccgtgc gagagaccac tcacgcgcgc tgttcgcatt ggtcatcgtt
      361 tccaaagtcg tgaacgtttt tgacgagatg atgaatagcg tttcttcggc ctggagatcg
      421 tggacagctt ccgagaagtc ggttccatcc acattcgaga cgaaccggaa ggtcatggtc
      481 cgctcgctgt aatgcttcag cgcttcatag gccatgacgg gtccgaggtc tgacccgccg
      541 atgccgatat tgacgacgtt tcggatcgcc ttgccggtgt ggcctttcca tgtgccgttc
      601 cggatgcggc gacaaaactc ggtcatctga tcaagcacct cgtgcacctt cgggaccacg
      661 ttctcgccgt cgaccatgat cgaggcttct cggggagccc ggagcgccac gtgcagcacg
      721 gctcgctgct cggtcacatt gatcttctcc cctcggaaca ttgcctcgat tcgctcgcgc
      781 agaccggact catccgccag ttgcagcaga agctccagcg tttggtcggt gatccggttc
      841 tttgagtagt ccagaagcag ccctacagct tccgctgtca gtcgagttcc tcgttgagga
      901 tcctctgcaa agagcgtccg cagatgcacg tcccggactg ctttataatg ggcttggagg
      961 gccttccacg atttacgcct cgggagcggc ttgatccgtg ccatcgtgga aggagccttg
     1021 cgtgttttcg tcgtcatgat cctctgattc tccaatcaaa ccgtcgcccg ttacggaacg
     1081 ggacagggtt ttacctccca aataccggcc gcatattccg cgatcgttcg atcgctggaa
     1141 aactttcctg atccggcgac gttcaggatt gccttctgcg cccaaccact cgcatccgca
     1201 tagagatcgc acagcgctcg gtcagccttc atgtacgagg tgagatctgc cagatgcatg
     1261 tagtggtcgc ttgtcagcac tgcctgttcc aatgcggcga acacgcccgg ttcattgcgg
     1321 ctaaagtaat tggagaagat caggtccagc gccgcccggg tttccggctc ctgttcatag
     1381 tgccagcgcg ggttatacca gtcgcgacta ctggccactt gctctgcggt caggccgaag
     1441 aggaagaaat tctcttctcc ggcttcttcc gccatttcaa tggtagcgcc gtcgcgggtc
     1501 cctaaggtca gggcgccgtt catcatgaat ttcatgttgc tcgtcccgct ggcctcatag
     1561 ccggccgtgg aaatctgatt ggagacatca gtggctggaa tcagtctttc ggctagcgac
     1621 acgcagtatt ccggcaggaa gaccactttg atcctgccac ggacgatcgg atctccatcg
     1681 atcgtgccag cgagattgtt gaggaacttg atgatgacct tggccaagtg ataggccggc
     1741 gccgcttttc cggcaaagaa gaaggtccgc ggagccatga ccatgttcga gttctcgcgc
     1801 agccggttat acagcacgac cacgcgaaga gcattcaaca gttgccgctt gtattcatgg
     1861 atccgcttca cctgacaatc gaagatactg ttcgggtcaa tgctctgccc cgatgtgact
     1921 ttgagccact gggcaaattg cagcttcgcc tcgcgttttg ccttgcgaaa agcgtcttga
     1981 aatgaccgat cgttggcaag ccttttgagc ttgcccagct cgccgagatc ggtaatccat
     2041 ccgtccccaa tggcatcggt gatcgcatgc gacagcgctg ggttggccat cagcaaccac
     2101 cgtcgagggg tgacgccgtt cgtcttgttg ttaaacctct cgggatagag ctcggccaag
     2161 tccttgaccg tcaccgtccg aagcagcttg gaatggatgg ccgccacccc gttggtgctg
     2221 tgggaaccca caatcgcgag attggccatc cggattttgc gcctcgcacc ttcttccaca
     2281 aggctgacac gtgccacgcg tccttcgtct cccgggaagc ggtttcgcac gacctcgatc
     2341 agacggcggt tgatctcaag aatgatctcc aactggcgtg ggagcatggc ctcaaaccat
     2401 tcgaacggcc atttctccaa cgcctcgggc aacagcgtgt ggttcgtgta ggccaacgtg
     2461 cgctgcgtga ggtcccatgc gtggtcccat ccaaggtgcg cttcatccaa tagaatccgc
     2521 atgagttccg gaaccgccat ggtgggatgt gtgtcattca actgaatggc cgctttctcc
     2581 ggcagtgcat tccaatcggg gtttccgcgc cggaagcgcc gcacgagatc ggccaaggag
     2641 caggccacca ggaaatactc ttgcaaaaag cgcaagccct gccccatgga tgtcgaatca
     2701 tccgggtaga gcacacgcgt gagcgattcc gccgtgagcg tttccgccaa cgcccccacg
     2761 aaatcaccac gactgaacaa ttgaaaatca aagtaatccg gagtgccggc tgcccacaga
     2821 cgcagtgtat tgatggtctt gccgccatag cccaccaccg gccggtcaaa ggggatgccg
     2881 agcagaacgg aaggacggcc cacaatggga cgcagaatgc cgctccgaag ctcgaacgaa
     2941 caattgagtt tcacctcgac cgcttcctcc aatcgtgcga cctcccaggg atcgggacgt
     3001 cgtaaccaat tgtccggctg ctcacattgc catccatctt taatggtttg cttaaagatt
     3061 ccgtactcat accgcaaacc atagcccatg cctggaattt ccatcgtcgc catcgaatca
     3121 atgaagcagg cggccagcct cccaagccct ccatttccca aaccggcatc aggctccatt
     3181 tcttgaatgg ccaacggatc gagtccaagc tgtttttgac gttccatcgc caatggccgg
     3241 agcaaggcat tggtgatgtt gttcgcaaac gaccgtccga gcaaaaattc catcgagagg
     3301 tagtacaccc gcttgggatt cttctccaca taggtgcgct cggtctgtag ccagcgttgc
     3361 gacacaacgt cgcggacggc atgtgccgcc gcttcaaact gctcccggac actggcctgc
     3421 ttgacatcaa cgacgttatc gaacgtg
//
LOCUS       DASTQY010000355         3664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_942264, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000355 DASTQY010000000
VERSION     DASTQY010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942264"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFR82_11620"
     CDS             complement(<1..301)
                     /locus_tag="VFR82_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4502290.1"
                     /translation="MMPQQVAYEVRSLFFRYGSAHDRTNWAVQDVSLQVKAGEILGIV
                     GPNGSGKTSLLKLLAKIVRPYTGEITLFGQSLELASHQEVARQVAFVPQDNQPAFS"
     gene            complement(338..1366)
                     /locus_tag="VFR82_11625"
     CDS             complement(338..1366)
                     /locus_tag="VFR82_11625"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU4502291.1"
                     /translation="MPRWLSVMAILSTAAIALTLLCLQMGTQYVSIAEVGRIIGRAIG
                     VGETEANGSSITDTIVLQLRMPRVLLGFLVGSSLSSVGVILQALLRNPLADPYILGVS
                     SGAALGVSLAVLFGLGGMAWLIPTLPIYGFFGGLLALMVLYRMSATYDRLPVHSVLLA
                     GVILNAIFSALIMFITSIMEPNRAFGMMAWLMGSLTATADHTLVALAIYLVLCLAWLF
                     KQVNVLNILTLGEEPARSLGVDTERAKRGILMTAALVTGAVVSVSGMIGFIGMVVPHA
                     VRLVLGADHRLLLPASALVGGMVLMTADTFARSFFAPSELPVGIMTALAGGPFFIYLL
                     VWRKDRLA"
     gene            complement(1480..2379)
                     /locus_tag="VFR82_11630"
     CDS             complement(1480..2379)
                     /locus_tag="VFR82_11630"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEU4502292.1"
                     /translation="MKRRQQGILTGMPFMANIAPRTFVDDSGRKLYFAKPPRRVLSLA
                     PSLTEMLFAIGLDEQIVGVTNFCNYPPAAAGKPKVGYTQPNLESLLALSPDMVAAPGE
                     LLRADILAKLEALKVPTFIFEAKSLEHVLAHIHTLGRIFERSSAADALTRHMRERIAE
                     VSRRVQTVERKRVLYVLSSHPLITVGPNSYIHQMIELAGGINVAAETSGAYPRLSMET
                     VLEKDPEVLVFPVGSTESVPEHEQQTWKRWTGLSAVQHQRLHVVSSDALNRPGPRVVD
                     GLEQLAQVIHPEAFSSDRTSSRP"
     regulatory      complement(2771..2966)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3174..>3664
                     /locus_tag="VFR82_11635"
     CDS             3174..>3664
                     /locus_tag="VFR82_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502293.1"
                     /translation="MKHLVLAIVILWAAFHFGGMFFSAKDRSPGVAFGRLLDAPQRSI
                     QNPYRNGYFYLIGLMAASSLDPGKTGYEIWVEETEEAGRGASGERKATRADLVFTLST
                     DTTNSAWEADDPLHAFGKKEAPFRAAASQYHILLARYERWLGMPFDDWGFGRKVVPLG
                     KHIL"
BASE COUNT          864 a         1058 c         1020 g          722 t
ORIGIN      
        1 aagaaaaagc cggctgattg tcttgaggaa caaacgcgac ctgccgtgcc acctcctgat
       61 ggctcgccaa ctccagagat tggccgaata acgtgatctc gcctgtgtac ggcctcacga
      121 tcttagccag aagcttgagc aaggacgtct taccagaccc gttcgggccc acgatgccca
      181 aaatctcccc ggccttgacc tgcaagctca catcctgaac agcccagttg gttcggtcat
      241 gcgcagaacc gtaacggaaa aacagtgacc ggacttcgta ggcaacctgt tgcggcatca
      301 tgaacactct cgtttcgatt tcctgttggc ggtcatgtca agccagccga tcttttctcc
      361 acacgaggag atagataaag aatggacctc cggccaatgc ggtcatgatt ccgacaggca
      421 gttctgaagg agcaaaaaaa cttctcgcga acgtatcggc cgtcatgagc accatgcctc
      481 ccacaagggc ggaggcgggc aacagcaagc gatggtcagc gccgagcacc aatcgcacgg
      541 catgaggaac aaccatcccg atgaagccaa tcatgccgct tactgaaacg accgccccgg
      601 tgacgagcgc ggcggtcatc aagatgcctc gtttcgcccg ttcggtatcg acacccagtg
      661 agcgggccgg ctcctctcct aatgtcaaga tgttcagcac gttgacctgc ttgaacaacc
      721 acgccagaca aagaaccaaa tagatggcga gggcaaccag cgtatgatcg gctgttgccg
      781 tcagcgatcc catcagccac gccatcatgc cgaaggcacg attgggctcc atgatcgacg
      841 tgatgaacat gatgagggcc gaaaaaatgg cattcaaaat gactcctgcc aggaggacgc
      901 tatggaccgg cagcctgtcg taggtcgctg acatgcggta gagcaccatg agagccagca
      961 gacccccgaa gaacccatag attggcaaag tcggaatcag ccaggccatg ccgccgagac
     1021 cgaacaggac ggccaaagaa acgcccagcg cggcgccgct cgagacgccg agaatgtagg
     1081 ggtcggctaa tgggttgcga agcagcgctt gtaaaattac gccgacggaa gacagggaac
     1141 tccccacgag aaatccgagc aacactcgcg gcattcgcag ctgcaagacg atcgtgtccg
     1201 tgatagacga tccattggcc tcagtctcac ccacaccgat ggctcgtccg ataatccgcc
     1261 cgacttctgc aatactcaca tactgggttc ccatttgaag acaaagcagc gtgagagcga
     1321 tggcggcggt tgacagaatg gccatcacgc taagccatcg gggcatggtg agaatggcct
     1381 tgcgaaacgt ggctgcatcg cagacagcgg ctgcgggtac gggtttcggc gatgacgctc
     1441 gatcggacag ggaggatgct ggctggctgg gcgaaattgt catggccgcg aagatgtgcg
     1501 gtcggaggag aacgcttccg gatgaatcac ctgcgccagt tgctccaagc cgtcgacgac
     1561 acgtggccct ggacgattca gcgcgtccga ggacacgaca tgaagtcgtt gatgttggac
     1621 ggccgacaaa ccggtccatc gcttccatgt ttgctgttca tgctcaggaa cactttcggt
     1681 tgatcccacg ggaaagacca gtacttcggg atctttctcc aacacggttt ccatgctgag
     1741 ccgaggatag gctcccgacg tttcagccgc gacgttgatt cctcccgcca gctcgatcat
     1801 ctgatgaatg taactattgg gaccgaccgt aattagcggg tggctgctta atacatataa
     1861 gacccgtttt cgttctacgg tctggacccg gcgcgagacc tcggcaatcc gttcccgcat
     1921 atggcgagtc agcgcatcgg cagcagacga acgttcgaaa atcctcccca acgtgtggat
     1981 gtgcgccagg acatgctcga gggatttcgc ttcaaagatg aacgtcggga ccttcaatgc
     2041 ttccagcttc gccaggatgt cagcacgcag aagttcaccg ggtgccgcta ccatatccgg
     2101 actgagggcc aacaatgatt caaggttggg ttgtgtgtag ccgactttag gcttgccggc
     2161 ggccgccgga ggataattgc aaaaattcgt gacgcccacg atctgttcat cgaggccgat
     2221 ggcaaacagc atttccgtaa ggctgggagc cagggagaga actcgacggg gaggcttggc
     2281 gaaatacaac tttcgacccg aatcatccac aaacgttcgc ggcgcgatgt tggccatgaa
     2341 cggcatgccg gtcaggatgc cttgttgacg tcgcttcata actgagccat caccttcatt
     2401 gcccagtgcg attccagcac aaaaaacccc tgttcccaaa cagctgatca accaagtagc
     2461 aagggctcgc tcaaacggat ttctgcgtaa ggccgcagcg agcaagtggc cgaggcgtac
     2521 tcttttctca cccgcccacc caccgcctgt cggaacaggc gtttccccgt cttgtacgcc
     2581 gcaggcgact ggcggagcga gaacgcagcg aagaatcagt ttcagcgagc ccaaataaaa
     2641 aatccccaag gcctggaaag accctgagga ttgcacccgc ttcttcatcc tcgcctgttc
     2701 cccagccctc gagggaacga tggtctaggg aaccgatgag aggatcacac gcgagtggcg
     2761 atagatccgc tgaaagattc ccgttccggg caggtcttct ggctcatggt tcatcctact
     2821 ccccgtgcct tcccatccgt cgaaccggac agtggcgtag gcgggtttcg tccccattga
     2881 cagcggcggg accgcgaggg atttgcaccc tcttccctta cccgaacatt acgattgtga
     2941 gcggcactct aggagagagt ggcatttttt gtcaagttca ataatgtccg attgcccagc
     3001 ctggcaacag tcagagacaa ggtcatagga acaacctgct gaaacggcac gactttttgg
     3061 gaccttgtgc ggcatagcgc ttgcctctta cagatgtatt gggagaacca ttcaagtcct
     3121 ccccttagag aagagccggt aaagaagccc ggcgtgatgc gtgggatgca aagatgaaac
     3181 acctcgtcct tgccatcgtc atactgtggg cggccttcca ctttggcggg atgttctttt
     3241 ctgcgaaaga ccgtagcccg ggtgtcgcct tcggccgcct gctcgatgcg ccgcagcggt
     3301 ctatccagaa cccataccgc aacgggtact tttatctcat tgggctcatg gcagcgtctt
     3361 cgctagaccc aggaaaaacc ggctatgaga tctgggtgga agaaaccgag gaagcgggcc
     3421 gaggcgcatc tggtgaacga aaagccacca gagccgacct cgtgttcact ctctcaactg
     3481 atacgaccaa ctccgcatgg gaagccgacg atccgttgca tgcgttcgga aagaaagagg
     3541 ctcctttccg agcggcagcc agccaatatc acattcttct cgcgcgatat gagcgctggc
     3601 tcggcatgcc gttcgatgat tggggtttcg gacgcaaggt cgttccactc ggcaagcaca
     3661 ttct
//
LOCUS       DASTQY010000356         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_772739, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000356 DASTQY010000000
VERSION     DASTQY010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772739"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFR82_11640"
     CDS             <1..482
                     /locus_tag="VFR82_11640"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502294.1"
                     /translation="TATFNGDGRHATEAALAGPSGLTIGTDGRLYIADTFNGRIRAVD
                     PATGMIETVVGDGGEYRYQGEMEAATPSLSRPCGIVVDLEGNVFMTDSDNHLVRRWER
                     VAGRIDRVAGIGIANYGGDGKTPLEASLNYPFGIVMDQQNHLFVADTFNHRIREIVL"
     gene            587..1609
                     /locus_tag="VFR82_11645"
     CDS             587..1609
                     /locus_tag="VFR82_11645"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU4502295.1"
                     /translation="MLKKKSSYVIAILGATGAVGEETLDILDERKFPLAGLRLFASKR
                     SAGEVLTCQGKEWTVEELTPEVSFAGVDIALISATDAISREYGARLGAAGVTVIDDSA
                     VFRMDLDVPLVVPEVNAQALGSMPRGIVAIPNCTTTPLVMALKPLRDAVGLKRVVVTT
                     FQSVSGTGAAAMEELITQTKDLMAFRDVKTEVYPYQIAFNLLPHIGSFSEEGDCSEEV
                     KIANETRKILDAPTLRVTATTVRVPVLRCHSEAINVELEGPLTSNEARAALAAMPGVI
                     VYDDPSRKLYPMPLDATGKDEVYIGRIREDVSVTNGLNLWVVSDNLRKGAALNAVQIA
                     ECLVKG"
     gene            1591..1863
                     /locus_tag="VFR82_11650"
     CDS             1591..1863
                     /locus_tag="VFR82_11650"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEU4502296.1"
                     /translation="MSGERLMIEWENVGKILIGVGTVVALLGVLFLLVDRVPALSHLL
                     SWLGRLPGDISIKRDNFSLYFPLATSIVLSVILSLLFYLLAWIFRR"
     gene            1896..>2837
                     /locus_tag="VFR82_11655"
     CDS             1896..>2837
                     /locus_tag="VFR82_11655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEU4502297.1"
                     /translation="MVVSVVTAPDAVLAAQSIRVLLSADVYRLDVQADGAVWLTDSSR
                     DGRAIRSDARITANGKGFLVNGTRIAHERLTLRAGERGLTLMLVKPNDKLPSVSKPIK
                     PVNDQRLAMPVSGLVHVVRKGKGFLVINQVDLEEYVKGVVPGEVNSSWHPEMLKAQAV
                     AARTYALYQQMLSASRDYDVVATVQDQVYKGKDGVDTAVLRAVDETRGLVLTYQQAPI
                     YAAFSSTAAGLTEDAVNVWSKDYPYLKGVECPFDLDSPFYQWKASFRMDKLEQNLRQQ
                     GYAVGTITSIEPLAFSRGGRVAKLRILHSEGELVLRGE"
BASE COUNT          592 a          747 c          888 g          610 t
ORIGIN      
        1 gcaccgccac attcaatgga gacggcaggc atgcgaccga ggccgcgctc gccggaccga
       61 gcggattgac cattgggaca gacgggaggc tctatattgc cgatacgttt aatggaagaa
      121 ttcgagccgt cgatccggcg accggcatga tcgagacagt ggttggtgac ggcggcgaat
      181 atcggtatca aggcgagatg gaagccgcaa cgcccagtct gtctcggcca tgcggcattg
      241 tggttgactt ggagggcaat gtgttcatga ctgattccga caaccatttg gttcgacgat
      301 gggagcgagt ggctggtcgg atcgatcgag tggccggcat tggcatcgcc aattatggag
      361 gagacggtaa aacgccgtta gaagccagtc tcaattatcc gttcggcatc gtgatggacc
      421 aacaaaacca tctattcgtg gcggatacat ttaatcatcg gattcgagaa atcgtactgt
      481 aaccagacgc ggcgttggca gccggccgac tgttcgctgg aagccgaggc gctcttggtt
      541 gagtcggtga aagacgcacg ttgcaacggg taggaacgcc tggtcaatgc tgaaaaaaaa
      601 gtcctcctat gtcatcgcca tcctcggcgc aaccggcgcg gtcggcgaag agacgctcga
      661 cattcttgac gagcgcaaat ttcccctcgc cggccttcgt ttgttcgcct cgaagcgttc
      721 agccggagag gtgttgactt gtcagggaaa ggaatggacg gtcgaagagt tgacgccaga
      781 ggtgtccttc gccggcgtcg atattgcctt gatttccgcg acggacgcta tcagtcggga
      841 atacggcgcc cgtttagggg ctgcgggggt cacggtcatc gacgacagcg cggtgttccg
      901 tatggacctg gatgtgccgt tggtcgttcc ggaggtgaac gcgcaggccc tcggttccat
      961 gcctcgcggc attgtggcga ttcctaactg caccacgacg cctttggtca tggcactcaa
     1021 gccactgcga gatgcggtcg gcctaaaacg ggtagtcgtg accacgtttc agtccgtctc
     1081 tggaactggt gcggcagcga tggaggaact gatcacccaa actaaggact tgatggcctt
     1141 tcgggatgtg aagaccgagg tctaccccta tcaaattgcc ttcaacctgt tgcctcatat
     1201 tggctccttt agcgaggaag gcgattgctc ggaggaagtg aagatcgcca acgaaacgag
     1261 gaagattctg gacgcaccga cgttgcgggt caccgccacc accgtgcggg taccggtgct
     1321 gcggtgtcac tcggaggcca tcaatgtgga attggaaggg ccgttgacgt ccaacgaggc
     1381 ccgggcagcc ttggccgcga tgccgggagt catcgtgtat gacgacccaa gccggaagct
     1441 ctatccgatg ccgctggacg cgacgggcaa agatgaggtg tacatcggac gcatcaggga
     1501 ggacgtatcc gtcacgaacg gcctgaatct gtgggtggta tccgacaatc ttcgaaaagg
     1561 cgccgcgctc aatgcagtgc agatcgcgga atgtctggtg aaaggctgat gatcgagtgg
     1621 gaaaacgtgg gcaaaatcct cattggtgtc ggaacggtgg ttgctctgct gggggtcctg
     1681 ttcctgcttg tcgaccgagt gccggccctc agccatttgt tgagctggct cggaaggctt
     1741 cccggcgata tttctattaa acgggacaac ttcagcctat attttccgct cgcgaccagt
     1801 atcgtcctca gcgtcatcct tagtctgcta ttctatttac tagcatggat cttccgccga
     1861 taacacgcct cacccgtctc agcctgtgga tagtcatggt cgtctctgta gtgacggcac
     1921 ccgatgcggt acttgcagcc caatcgatcc gcgtgctgct ctcggccgat gtgtaccggt
     1981 tagacgtcca agccgacggc gccgtatggc tgacggattc aagccgcgac gggcgtgcga
     2041 ttcgctcgga tgcccgcatt acggcaaatg gcaagggatt tctcgtcaac ggcacccgta
     2101 ttgcccacga acgcttgacc ctgcgggcag gagaacgagg gttgaccttg atgttggtga
     2161 agccgaacga caagctgccg tcggtgtcaa agccgatcaa gccggtgaac gatcaacgcc
     2221 tggccatgcc ggtgagcgga ttggtgcatg tggtacgaaa aggcaagggc ttcttggtca
     2281 tcaatcaggt ggacctcgag gaatacgtca aaggtgtggt cccgggtgag gtcaattctt
     2341 cctggcaccc ggagatgctg aaggcccagg ccgtggctgc acgtacgtat gcactgtatc
     2401 aacagatgtt gagcgcctca cgagactatg acgttgtggc aacggttcag gaccaagtct
     2461 acaaagggaa ggatggcgtc gatacggcgg tgttgcgtgc ggttgacgag acccggggtc
     2521 tcgtgttgac ctatcaacaa gcccctattt acgccgcctt ttcctccacc gccgctgggc
     2581 tgaccgaaga tgcggtgaac gtctggtcga aggactatcc ctatctcaaa ggcgtggaat
     2641 gtccgttcga tctggactct cccttctatc aatggaaggc gtcgttccgg atggataagt
     2701 tagagcagaa tctgcgccag caagggtatg cggtcgggac gatcacatcg atcgagccgc
     2761 tggcattcag tcgtggcggc cgtgttgcga agctgcgaat ccttcattcg gagggagagc
     2821 tcgttttgcg cggggag
//
LOCUS       DASTQY010000357         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_877033, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000357 DASTQY010000000
VERSION     DASTQY010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877033"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VFR82_11660"
     CDS             <1..589
                     /locus_tag="VFR82_11660"
                     /inference="COORDINATES: protein motif:HMM:NF013035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HEU4502298.1"
                     /translation="LSNRILAGALTFIGLGLIWVAIVSSLPSEQELAAKEAPIVPRAV
                     SKSLPEPTLDLSIPGVPATQPAAVPLTTVAAERPFVSKRLDPRVAQLSRLRCEAEVEQ
                     LCPESLDGTARKQCFEQRTPQLSVSCQHQVRQRVVKWKEERSRLMAACQVDIKRFCPM
                     KPGGGELLQCLQQHAQELSDGCYERLPKGALHLKY"
     gene            complement(596..1297)
                     /locus_tag="VFR82_11665"
     CDS             complement(596..1297)
                     /locus_tag="VFR82_11665"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU4502299.1"
                     /translation="MGTVTNSAKLLVKLQGQGSTLVELERDSFTIGRRPDNDLSIEDH
                     TVSSRHAKIIRVQSVYFLEDLKSTNGTAVNGQAIERAQLHDADVITIGQHRIIFQDSG
                     TIPACASSTSTGFDQTMAIPSRSVQGGPSITARILVTAGKTDRLEYSLTKPSNLIGSQ
                     DGATIRLTGWFAPKTAALISGRAGGFTVSPSQCNKPLLVNGHPVTVQQQLKDGDVIEV
                     AGVSLTFYVVQPNVR"
     gene            complement(1317..>2102)
                     /locus_tag="VFR82_11670"
     CDS             complement(1317..>2102)
                     /locus_tag="VFR82_11670"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU4502300.1"
                     /translation="GRKRSHNEDCYAADPALGLYVVCDGMGGGNAGEVASHMAIDAIL
                     AHVKAEAQRGSVAGRGTVNRNLSPVTNQLADAIRAANETVYRASWEQAKYAGMGTTVV
                     AARLTERHVSVAHVGDSRLYLIRNGAIQTLTSDHSWVAEQVEKGYMTEEEAERSPRRN
                     IVTRALGVESAVDIDLAEIPIANGDLLLLCSDGLTRGVRCRDILNTLEAPGDLCVKVQ
                     RLVSQANDAGGDDNITVLLVRVQESAAGRLWHRLAPQWLLNAS"
BASE COUNT          406 a          629 c          622 g          445 t
ORIGIN      
        1 gctttccaac cggattctgg ccggcgcgct caccttcatc gggctggggc tgatctgggt
       61 tgccatcgtc agcagcctgc cttcagagca ggagctcgct gcgaaggaag cgccgatcgt
      121 accgcgggca gtgtcgaaat cccttcctga acccacactc gatctgtcga ttcccggtgt
      181 gccggcaact cagcctgccg cagtgccgct gacgactgtc gccgcagaga ggccgttcgt
      241 ctccaagcgg cttgatcctc gtgttgccca actatctcga ctgcggtgtg aagccgaagt
      301 tgaacagctc tgccccgaat ctctggatgg aaccgctcgc aaacagtgct ttgagcagcg
      361 cacgccacaa ctgtcggtgt cttgtcagca tcaggtgcgc caacgcgtgg tcaaatggaa
      421 ggaagagcgg agccgcttga tggccgcctg tcaggtcgat atcaagcgat tttgtcccat
      481 gaaaccagga ggcggggaat tgctgcagtg tctccagcaa catgcccagg agctgtcgga
      541 cgggtgttat gagaggcttc cgaaaggggc gttgcacctc aagtactaga atccgctatc
      601 tgacattcgg ctgcacgaca tagaatgtca acgagacccc ggccacctcg atcacatcgc
      661 catccttcaa ctgctgttgc acggtcacgg gatgtccatt cacgagcagc ggtttgttgc
      721 actgcgacgg gctgacggta aaaccgcctg ctcgaccgga aatcaatgcg gccgttttgg
      781 gagcgaacca gccggtcaag cggatggttg cgccgtcctg cgacccgatc agattagagg
      841 gcttggtgag cgaatactct aaacggtctg tctttcccgc ggtgaccagg attcttgccg
      901 tgatcgacgg cccaccttgc acagatctgc taggaatggc catcgtttga tcgaaccccg
      961 tagaggtcga tgaggcacac gcagggatcg taccactgtc ctgaaaaatg atgcgatgct
     1021 gaccgatcgt gatgacgtcg gcgtcatgca gttgcgcacg ctcaatggct tggccgttga
     1081 ccgccgtgcc gttcgtgctc ttaagatctt ccaggaagta gacggattgc actcggataa
     1141 tcttcgcgtg gcggcttgac acagtgtgat cttcgatcga taaatcgtta tccggccttc
     1201 gaccgatcgt aaaggaatcc cgttcaagct cgaccaaagt ggagccttgg ccctggagtt
     1261 tgaccagaag tttcgccgag ttggtgacgg tgcccatcgt gcgctccgga tgaaggtcaa
     1321 gacgcgttca acaaccactg tggcgcgagg cggtgccaca gtcgacctgc cgcgctctcc
     1381 tggacgcgta ccaacaacac ggtgatgttg tcgtctccac cggcgtcgtt cgcctgcgag
     1441 accaatcgct gcactttgac gcacagatcc ccgggcgcct ctaaagtgtt gaggatgtcg
     1501 cggcagcgga cgccccgggt gagaccgtct gagcacagca gcaggaggtc accgttcgcg
     1561 atggggatct cggcaagatc gatgtcaacg gcgctctcga cgcccaacgc tcgagtgaca
     1621 atgttccggc gcggtgaacg ctccgcttcc tcttcggtca tgtagccttt ttccacttgt
     1681 tcagcgaccc acgaatggtc gctcgtcaag gtttgtattg caccgttgcg gatcaagtac
     1741 agccgactgt ctccgacgtg ggcgacggac acatgccgct cggtcaggcg ggcggcgacg
     1801 acggtggttc ccatcccggc gtacttcgct tgttcccaag aagctcgata caccgtctcg
     1861 ttggcggcgc ggatggcatc ggccagctgg ttggtcacgg gagataaatt gcggttgacc
     1921 gtgcctcgac cggcgacaga ccctctttgc gcctcggctt tcacgtgggc gaggatggcg
     1981 tcgatcgcca tgtggcttgc cacttcgccc gcattgccgc cccccatccc gtcacaaacg
     2041 acatagagac cgagtgccgg atcagccgca tagcagtctt cgttatgaga ccgcttgcgt
     2101 cc
//
LOCUS       DASTQY010000358         7674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1482010, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000358 DASTQY010000000
VERSION     DASTQY010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482010"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..346)
                     /locus_tag="VFR82_11675"
     CDS             complement(134..346)
                     /locus_tag="VFR82_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502301.1"
                     /translation="MSDNDALYAIRHPDGSVSLYIDEEYAAERGVDPATLTRVEIPRE
                     LFTSGTVQQVREYVAVFLEAQKTGTA"
     gene            complement(428..1057)
                     /locus_tag="VFR82_11680"
     CDS             complement(428..1057)
                     /locus_tag="VFR82_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502302.1"
                     /translation="MLSILIPGTGHVLQKRYPQALSACLLTLALIIASLALGRVSGRA
                     AEVFFFMVLALPWWALQGYDAYLGPPETGSTWRRTFRTAWRRGHDIRFLGVLLVISAL
                     NDTFIILANLDYLLPFYCTKPTGIPGFLTKAISPVLHLSVGYGFIRCRRWAFFLYLVY
                     AAYGFTNGMVNLTCFGPGRIRNTLLGAVVLSTIYVLFRRHVLLHQPPPP"
     gene            complement(1161..1883)
                     /locus_tag="VFR82_11685"
     CDS             complement(1161..1883)
                     /locus_tag="VFR82_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEU4502303.1"
                     /translation="MAAPEQQPAQQAPKRQYVNFAFYKLDPAWRRLPEDERTKGKQEF
                     VRAVEDYSGKVLVIPYTTVGIRGDCDFLLWRISYELELFQQMSSKILASGLGKYLSTP
                     YSYLSMTKRSIYVDHHTHEGQESKRLTVVPGKAKYIFVYPFLKTREWFLLTKAARQGM
                     MDEHIEVGHRFPSVKLNTTYSFGLDDQEWVVAFESDKPEDFLDLVMALRETEGSRYTL
                     RDTPIFTCIRGSLKETLDTLGG"
     gene            2027..2626
                     /locus_tag="VFR82_11690"
     CDS             2027..2626
                     /locus_tag="VFR82_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502304.1"
                     /translation="MKASVRATTGAAVACFVIGTAAGASAIETHPSQEQIQSALARGA
                     EAAKQHSPPDTFYTRFGAADDLHPSGFLITKLGALAVMATHMGLRGVEPAEADIAQSL
                     DGKTMLVSATIFGNVPNFAVDSYMVLDQSGKTIKPVTVRFDGQAHRSAAWPEPPRFKA
                     KVVASFAYGDFDPVAQTTITIFPANGGEVTFPIDFAQIQ"
     gene            2771..4054
                     /locus_tag="VFR82_11695"
     CDS             2771..4054
                     /locus_tag="VFR82_11695"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU4502305.1"
                     /translation="MRRARTSRTPPIAETIAVGSELLLGGRTDSNSLCITDELAMLGI
                     EVRFKSVVGDDRADIVHALNTAVQRARVIVMTGGLGPTVDDCTREAVAQATGRRLARR
                     KEAFEGMQARLAQWRRVPNKGQLRQALIPSGASVVPNPVGSAPGFWLSWRGALLISLP
                     GVPGEMAAMMRETVVPLLKSHVPQALGRSIRRLIFHTWGISEADVDAKLLGLIPKAVP
                     VDLGLLASPMGVLVSLTMKPRAAAGEETLVSLSNELRVRLQEWIFAEDQESMEEVVGQ
                     LLTKQHRTVALAESCTGGLIGHRLTQVPGSSIYMDRGVICYSNRAKVELLGVRPELIE
                     QHGAVSAEVAAAMAKGVRERSGVSVGLSVTGIAGPGGGTAAKPVGLVYIGLNADDGAS
                     QVKEHRFHGDRTVIKQRASQAALDLLRRWLLTGHP"
     gene            4153..4755
                     /gene="thpR"
                     /locus_tag="VFR82_11700"
     CDS             4153..4755
                     /gene="thpR"
                     /locus_tag="VFR82_11700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEU4502306.1"
                     /translation="MIRTFLAVELSEPLRTNLATIQSDLKQRIAKALTRDVSISWVRP
                     ASLHVTVKFLGDIDEELVPPLKEAITELLRAHGPIEVPVNRLGVFPRPQQPRILWVGP
                     SEQWESGEDAARLAALHRAIDACCGSLNLATDSRPLSAHLTLARVKAGERVVGQVLAQ
                     SGVMDRPFTCESLDINAIALMKSELKPSGSVYTRLWDVRL"
     gene            complement(4977..6062)
                     /locus_tag="VFR82_11705"
     CDS             complement(4977..6062)
                     /locus_tag="VFR82_11705"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502307.1"
                     /translation="MSHASVQETVDRKPTVLIVDDEAAPRAALTQILKEDFNILTAKD
                     GRHALAVLNDRGVDLITLDLKLPDTSGADILKEIKRTHREIEVIMVTAYGSLQSAMDC
                     VRHGAAGFLLKPFNAGELLSISLQTSQKKQRLDMLREAMATQEALWQSDPGCTEAWQA
                     LMARYARQVKSGLLEVSSREVTSPLIQLVSDLLEARDRHLVNHGNRVSFYATLVGNKL
                     QLPLPEQQYLSLGALAHDLDLICHDSSPVLHHDSTPTHHRPDLGARIGRAIGLPSEAV
                     QIIALHHERWDGTGYPFALKEERIPYLARLVSLAQTFDELTAERPGAVPVPFKEALEV
                     IEAQSGTAFDPSLVEVFCRTIREQPPK"
     gene            6181..6573
                     /locus_tag="VFR82_11710"
     CDS             6181..6573
                     /locus_tag="VFR82_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502308.1"
                     /translation="MKWLAVITVLVVALCTTAWAGESGGFDPDQPFKEAFSQRGLQSF
                     LNQALEALEDHFEITGNLNPDSDAADRTGKLQFKFYPEGKSKSNDHVTAEGWFGPSKD
                     SLQDEFHFRFAVPKSSSKPSAEPLSNVL"
     gene            6687..>7674
                     /gene="recA"
                     /locus_tag="VFR82_11715"
     CDS             6687..>7674
                     /gene="recA"
                     /locus_tag="VFR82_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU4502309.1"
                     /translation="MAEKDDKKRALDLALSQIEKQYGKGAIMKLGDAEAPADIPAISS
                     GSLGLDIALGVGGFPRGRVIEIFGPEASGKTTLTLHAIAEVQKTGGVAAFIDAEHALD
                     LTYARKLGVQADDLLVSQPDTGEQALEIAETLVRSGAIDVIVVDSVAALTPRVEIEGE
                     MGDAHMGLQARLMSQALRKLTGAISKSQTTVIFINQIRMKLGVMFGNPETTTGGNALK
                     FYSSVRLDIRRIESIKEGQEVVGSRVRVKVVKNKMAPPFKQAEFDILFSQGISKAGEL
                     VDLGVDKKVLEKSGAWYSYHGERLGQGRDAVRDFLLTNQPLAREIEGKLRDLA"
BASE COUNT         1623 a         2082 c         2373 g         1596 t
ORIGIN      
        1 aaatgctgaa gcaagatgag gcgcagcatg atccggcctt gaatcggcag gctgctgatg
       61 acggattcga tcaattccgg agtgagcggg gaggggctgg gagcggtcat ggttgccata
      121 aaaatagttc cagttacgcc gttccggttt tttgagcttc gaggaagacc gccacatatt
      181 cgcgcacttg ctgtacggtc ccgctcgtaa acagctctcg ggggatctcc actcgtgtca
      241 gcgtcgcagg atcgacccct cgctcagccg catactcctc gtcgatatag agactgaccg
      301 acccgtcggg atgacgtatg gcgtagaggg cgtcgttatc ggacatatca aggcgtctga
      361 ctgtttgctc tacaggtatc acaggggtct cgaagctgtc aaagcagggt gggtatgggt
      421 tggacgacta tggaggtggt ggttggtgta acaagacatg ccggcggaag agcacataga
      481 tggtcgagag gactacggca ccaagcaagg tgttgcggat gcgtccgggg ccgaagcagg
      541 tcaggttcac catcccgttc gtgaagccgt acgcggcata gacgaggtat aggaaaaagg
      601 cccatcgcct gcaccggata aagccgtatc ccactgacag gtgtaggacg ggagagatgg
      661 ctttcgtcaa gaagccgggg atgcccgttg gcttggtgca ataaaaggga agcaggtagt
      721 ccagatttgc gagaatgatg aaggtatcgt tcagcgcgct aatgaccagg agcacaccga
      781 ggaaccggat atcgtgcccg cgccgccaag ccgtacgaaa ggtgcgtctc caagtggagc
      841 cggtttcagg cggcccgaga taggcatcat agccttggag tgcccaccac gggagggcga
      901 gcaccatgaa gaagaacact tccgccgcac ggccggatac acgcccgaga gccaagctgg
      961 cgatgatgag tgcaagggtc agaaggcagg ccgaaagcgc ttgagggtag cgcttctgta
     1021 gaacatggcc cgtgcctgga atcaggattg aaagcacggc ggcgcctacg tcgcgacatt
     1081 cagacatgcc aaaaaagaag gggctgtcga tcgcacgatc gacagcccct catgtcaacg
     1141 agcctcacgt ccggacccgg ctacccgccg agcgtatcca gcgtttcttt caagctgccg
     1201 cggatacagg taaagatggg tgtgtcccgc agcgtatagc gcgagccttc ggtttctcgc
     1261 agcgccatga ccaaatccag aaaatcctcc ggtttgtcgc tctcgaatgc cacgacccac
     1321 tcttgatcgt ccaggccgaa tgagtaggtg gtgttgagct tcaccgaagg aaagcgatga
     1381 ccgacttcaa tatgttcgtc catcatgccc tggcgtgcgg ctttcgtgag gaggaaccat
     1441 tcacgcgttt tcagaaacgg atagacgaag atgtacttgg cctttcccgg gaccacggtc
     1501 aggcgtttac tttcctgtcc ttcgtgggtg tgatggtcga cataaatcga ccgcttcgtc
     1561 atggacaggt aggaataggg cgtcgaaaga tacttgccca ggccggaggc taggatcttg
     1621 gaactcattt gttggaagag ctcgagttca tagctgatgc gccacagcag gaaatcgcaa
     1681 tcaccacgga tgccgacagt cgtgtacggg atgaccaaaa ctttccccga ataatcttcc
     1741 acggcgcgaa cgaattcctg tttgcctttc gtccgttcat cctcgggaag gcgccgccag
     1801 gccggatcga gtttgtagaa tgcgaagttg acgtattgcc gttttggcgc ctgttgcgcc
     1861 ggttgttgtt cgggggctgc catgaactcc tccgtgtgat ggcttgatcg aaaaagtggc
     1921 gtgaagagcc aattctctag catttcgcct tttgctttgt caacggtcgc tggggcttga
     1981 gggccgctcg ttgacaggca aaaactgatc tgctactggc ttgcatatga aggcgtcagt
     2041 acgagcgacg acgggggcgg ctgtagcatg ttttgtcatc ggaacggcag ccggcgcttc
     2101 agcaatcgaa acccatccat cccaggaaca gattcagtcg gcactcgcgc gaggcgccga
     2161 agccgctaaa caacacagtc ctcctgatac gttttacacc cgattcggtg ccgcggatga
     2221 cctgcatccc agcggttttc tgatcacgaa gttgggcgca ctcgccgtga tggccacaca
     2281 catggggctg cgcggcgtcg agcccgctga ggccgacatt gcccagagcc tggatgggaa
     2341 gaccatgttg gtgagtgcga cgatcttcgg caacgttccc aattttgccg tcgacagtta
     2401 tatggtgctc gatcaaagcg gaaaaacgat caagcccgtg accgtgcggt ttgatggcca
     2461 agcccaccgc agtgccgcct ggccggaacc gccccgcttc aaggcaaagg tcgtcgcctc
     2521 cttcgcgtac ggcgatttcg acccggtggc acagaccacg atcacaattt ttcctgccaa
     2581 cggcggtgag gtgacctttc ccattgactt cgcccagatc caataacagg ttgggaattc
     2641 tcacgcaccc agccccgacc gtgtttttga cttttccccg tccgattgcc tggcaatcga
     2701 tttcgcggga cgcttcctac acagggcagc gttttcgcaa tccgttgaat gtccttctag
     2761 actgatatcc atgcgtcgag cacgtacctc caggacaccg cccattgccg aaacgattgc
     2821 tgtcggctcg gagttgcttc tgggcgggcg gacggacagc aattcgctgt gcattacgga
     2881 tgaattggcc atgctcggca tcgaagtgcg cttcaagtcg gtggtgggag atgatcgagc
     2941 cgacattgtc cacgctctca acactgcggt tcaacgggcg cgggtcatcg tcatgaccgg
     3001 cggcctcggg ccgacagtgg acgattgcac gagggaggca gtcgcacagg cgaccgggcg
     3061 gcggctcgcg aggcggaaag aagccttcga agggatgcag gcccgcttgg ctcagtggag
     3121 acgagtcccc aacaaaggac agttgcgaca ggcactgata ccgtccggag cgtcggttgt
     3181 gccgaatccc gtcggatctg cgcccggatt ctggctgtcc tggcgcggcg ccctgctgat
     3241 ttcgctgccg ggcgtgccgg gcgaaatggc cgcgatgatg cgggagacgg ttgtgccctt
     3301 gttgaagtct cacgtgcctc aggccctcgg ccgttcgatt cggcgtctga tctttcacac
     3361 atggggaatt tccgaagccg atgtcgatgc aaagttactg ggactcattc ctaaggcagt
     3421 ccctgtcgat ctcggactgt tggcttcgcc gatgggagtt ctggtctcac tcaccatgaa
     3481 gccgcgggcc gcagccgggg aagagactct ggtctcgtta tcgaatgagt tgcgcgtccg
     3541 tctgcaggaa tggatttttg ccgaagacca ggagtcgatg gaagaggtgg tcgggcagtt
     3601 gctcacgaag caacatcgga ccgtggctct ggcagaatcc tgcaccggcg ggctgattgg
     3661 tcatcgcctg acgcaggttc cggggtcctc catctatatg gaccgtggcg tgatctgtta
     3721 cagcaatcgc gcgaaggtcg aactgttggg tgtgaggccg gaactcatcg agcaacacgg
     3781 tgcggtgagc gccgaagtcg cggcggcgat ggcgaagggc gtgcgagagc gcagcggcgt
     3841 gtcggtcgga ttaagtgtca cgggcattgc cggaccgggc ggtggaacag cggcgaagcc
     3901 cgtcggactg gtctacatcg ggctaaatgc agacgacggc gcgtcgcagg taaaggaaca
     3961 tcgatttcat ggcgaccgga ctgtgatcaa acaacgcgct tcccaggcgg cgctggatct
     4021 gctgcggcgt tggttgctga cagggcatcc ataaacgccg ctggcggcga tctcccgtcg
     4081 ctgagcgtca accggaaaaa cccgtggaat agcagcgctt tcctagacaa cctgcagggg
     4141 gcatgagaga gcatgatacg cacgttttta gccgtcgaac tttcggaacc gctaagaacg
     4201 aatctcgcca ccatacaatc ggacttgaaa cagcgcatag ctaaagcact gactcgagac
     4261 gtgagcatct cgtgggttcg gccggcctcc ctgcatgtga cggtgaagtt tctcggcgat
     4321 atcgatgaag agttggtccc tcctctgaag gaagccatca cggaactgct gagagcgcat
     4381 ggtcccattg aggttcccgt gaaccgcctc ggcgtgtttc ctcgtcctca acagccgaga
     4441 atcttgtggg tagggccttc ggaacaatgg gagagcggag aggacgcggc gcgattggcg
     4501 gcgctgcacc gtgcgatcga tgcctgctgt ggatcgctga atctggctac cgacagccgg
     4561 ccattgagcg cgcatctgac cttggcccgt gtgaaggccg gtgagcgggt cgtcggacaa
     4621 gtcctcgcgc agagcggcgt gatggatcga ccgttcacct gcgagtcgct ggacatcaat
     4681 gcaattgcct tgatgaaaag cgagctgaag ccgagcgggt ccgtctatac acgattatgg
     4741 gacgtgaggc tgtgaggatg gtaagactgc ctgcttaagc gacgggttca aacagcatga
     4801 tctgtcactg cttggctcga caactcgttc cgtcccggct ttccgaacgg aacatcgaac
     4861 aagtctcgcc atcgcttcgg cgaacgcagt gacagcccat gcgacccatc gcagtcgcag
     4921 tccagcttta ccatcctctt cagtgaaaag aaaaataggg cggaaagacg gcaatcttat
     4981 ttgggtggct gttcgcgaat ggtgcggcag aacacctcta cgagggaagg atcgaatgcg
     5041 gtgccggatt gcgcttcgat gacttcaagc gcctctttaa agggtacagg cactgcgccg
     5101 ggccgttcgg cggtgagttc gtcgaacgtt tgggcaagtg agacgaggcg agccaagtag
     5161 ggtatccgtt cctctttgag cgcgaatgga tagccagtgc cgtcccagcg ttcatgatgg
     5221 agggcgataa tctgcacggc ttcagaaggc aggccgatgg cccgcccgat gcgcgcaccg
     5281 agatccggac ggtggtgcgt cggggtgctg tcatggtgca acacgggact gctgtcgtgg
     5341 cagatcaggt ccaagtcatg agccagagcc ccaagcgaaa ggtattgctg ctcaggcaac
     5401 ggcagctgca gcttattgcc cacgagtgtc gcgtagaagc tcacacgatt gccgtgattg
     5461 accaggtgtc gatccctcgc ctccagtaag tccgagacca gttggatcag gggtgacgtg
     5521 acttcccggc tcgacacttc gagcaagccg ctcttgacct gtctggcgta tcgggccatg
     5581 agggcctgcc aggcctccgt gcagccggga tccgattgcc acaaggcctc ctgcgtggcc
     5641 atcgcctccc gaagcatatc gagccgttgc ttcttctgcg aggtctgcaa gctgatagac
     5701 aacagctccc cggcattgaa gggtttgagg aggaagccgg cggcgccgtg acgcacacaa
     5761 tccattgcgg attggagcga accgtacgcg gtgaccataa tcacctcgat ctcacgatgt
     5821 gttcgcttga tttcctttaa gatgtcggct ccggaggtgt cgggaagttt caggtccagt
     5881 gtgatcaggt cgaccccgcg atcattgagc accgcaagcg catgacgccc gtctttggcc
     5941 gtcaggatat tgaagtcctc ttttaaaatc tgtgtcagag ccgcccgagg cgccgcttca
     6001 tcatcgacga tcaggaccgt cggtttccgg tcgacggtct cttggacgga agcgtgtgac
     6061 ataccttcag ctgtatgctg acaagccact ttcgtcaagg aaatgacgat tgggcgttgt
     6121 aggagaaagc ctgagcgagg cgtacttgac tgacccgact tcacattata gagtgtcacc
     6181 atgaagtggc tggcggtcat aacggtgctg gtcgtcgcac tctgcacaac agcctgggca
     6241 ggggaaagtg gcgggtttga tccggatcag cccttcaaag aagcgttcag tcagcgaggg
     6301 ctgcaatcat tcttgaacca agcgttggag gcacttgagg atcatttcga gatcaccggc
     6361 aatctcaatc cggattctga tgcggccgac cggaccggca agctgcaatt caagttctat
     6421 ccggaaggaa agtccaagtc gaatgaccat gtgaccgccg aagggtggtt tggtccctca
     6481 aaggattcgc ttcaggatga atttcacttc cggtttgccg tgcccaagtc gtccagcaag
     6541 ccatccgctg aaccgttgag caacgtgctg tagccctctc ctccgcattg ccaccatttt
     6601 ttcgtcccgt cgcattctct gctaggtttc tttccgctaa cttgggtata atccgccacg
     6661 cttcaccctg atgaggagga ttttccatgg cggaaaaaga cgacaagaaa cgcgcgttgg
     6721 atctcgctct ctcccagatc gagaagcagt atgggaaagg ggcgatcatg aagctcggcg
     6781 atgccgaggc gccggccgac atcccggcga tttcttcggg atcgctcggc ttggacatcg
     6841 cgcttggcgt cggtggattt ccgcgtggcc gcgtgattga gattttcggt cctgaagcct
     6901 cggggaagac gacgctgacc ctgcatgcga tcgccgaagt gcaaaagacc ggcggagtcg
     6961 ccgcgttcat cgatgcggag catgcgctcg atttgacgta cgcccgaaaa ctgggcgtgc
     7021 aagccgacga tctattggtc tcacagcccg acacgggcga gcaggcgttg gaaattgccg
     7081 agacgctggt tcgcagcggg gccatcgatg tgatcgtcgt ggattcggtg gctgccttga
     7141 ccccgcgggt ggaaatagaa ggcgaaatgg gcgatgcgca catgggcttg caggcgcgcc
     7201 tgatgtctca agcccttcga aaactgaccg gtgccatctc aaaatcacaa acgacggtaa
     7261 tcttcatcaa tcagattcgg atgaaacttg gggtgatgtt cggcaatccg gaaacgacga
     7321 cgggcggcaa tgcgctcaag ttctactcgt ccgtacgact ggacattcgc cggatcgaat
     7381 cgatcaagga agggcaggag gtggtcggta gcagagtccg cgtgaaggtt gtgaaaaaca
     7441 agatggctcc ccccttcaag caggcggagt tcgacatctt gttctcacag ggcatttcga
     7501 aagccggcga gctggtggat cttggcgtgg ataagaaagt gttggaaaag tccggcgcct
     7561 ggtattccta ccacggcgaa cgtctcggac agggccgtga tgcggtccgc gactttttgc
     7621 tgactaatca acccttggct cgcgagatcg aaggcaagct tcgggacctg gcgg
//
LOCUS       DASTQY010000359        11385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1736679, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000359 DASTQY010000000
VERSION     DASTQY010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11385
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736679"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFR82_11720"
     CDS             complement(<1..299)
                     /locus_tag="VFR82_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502310.1"
                     /translation="MCRNVMKGILLSLCSLAILSCSGAPKERKIEFVYMALGASDATG
                     VGAIPLTEGYVYLINRGLQQRIPGTVLINRGVPGARIDLIKEQVRLAKQLGTKAD"
     gene            complement(356..1009)
                     /locus_tag="VFR82_11725"
     CDS             complement(356..1009)
                     /locus_tag="VFR82_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4502311.1"
                     /translation="MPLDRQATRVMLVDDHPLVLESLTKLLDPHFMIVSTVQHSGDIV
                     SSALEHRPDVILLDACMPGLSGFEATRELKKLLPKLKVILVTMLTEAISVSEAFRAGA
                     NGYVLKQSASDELRTAIDTVLAHKRFLSNTIEPQVREAVEHEWFRPEGYSGDLTGRQR
                     EVLALLSEGRTTRNIAQQLNISRKTVEFHKANITHKLGVHTTSDLIKFALAQGITTL"
     gene            complement(1036..1398)
                     /locus_tag="VFR82_11730"
     CDS             complement(1036..1398)
                     /locus_tag="VFR82_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502312.1"
                     /translation="MTTMLATLVNEEDAVLDHSYGSSIGTTASGDINACLIARIGWCH
                     REAVKALTIAEAEEWRAEEQGLIDAFLGRDSGYEYQDRPIFKERYTMGLEDGRVLILT
                     ARIRPTCGHKYPSHVGQL"
     gene            1809..2210
                     /locus_tag="VFR82_11735"
     CDS             1809..2210
                     /locus_tag="VFR82_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4502313.1"
                     /translation="MELDQTPSNGSQAIRVLLVDDHAMLRQGLRNLLQNYGNVEVVGE
                     AADGEEAVTLATRLDPSVVVMDINMPKMNGIEATTRIHGQAPHLPIIGLSVNGIEHGN
                     AILRAGAKAFVSKEAAVDHLYVAILNAVNCS"
     gene            2653..2916
                     /locus_tag="VFR82_11740"
     CDS             2653..2916
                     /locus_tag="VFR82_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502314.1"
                     /translation="MRKRRIIVKRRPRSTAHEWERGWDLAMTIIDGVPPLVEKQPIFQ
                     DCLTVLDGAFADGNSLRFELGLSALIDFCNETVSRGECTQWWS"
     gene            complement(3153..3749)
                     /locus_tag="VFR82_11745"
     CDS             complement(3153..3749)
                     /locus_tag="VFR82_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502315.1"
                     /translation="MNRGFFHDSIERIVRLLPRPMMDGKLIAKSRNFWVIVEEYGEQD
                     GIVVLNKFTDHEGKIPYDSIRKWQEPDKVILSAQVTVGKNGLFDLNRFLDGPENEMIT
                     EEEEILPARMAHAVAALRGCTEEEIEVLKELLIKQSMEEGEIIVCCARHGIQSGYKFV
                     ATVNGRTSFLEMGERQKVWIKPAFVPILEKVLLRPIPS"
     gene            4005..4277
                     /locus_tag="VFR82_11750"
     CDS             4005..4277
                     /locus_tag="VFR82_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502316.1"
                     /translation="MNGRILLIAMLLPGVAGCESYSWSVYNNPSITDIKQGQDCRVLV
                     FGLGGTVDPTGIQAMRLGGVTKVRSTEYRVNTIQGVGNECIIAHGE"
     gene            complement(4372..4698)
                     /locus_tag="VFR82_11755"
     CDS             complement(4372..4698)
                     /locus_tag="VFR82_11755"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4502317.1"
                     /translation="MASIAWADMGKTAPALPAPSGSKAESAIKEGMMHYEQGHWDVAK
                     KHFEKAVQADDQSAEAHYDLALALDKSGDHQAAIQHFKAAQQYGKDNPEIQNSGILQA
                     HLKAKH"
     gene            complement(5037..6107)
                     /locus_tag="VFR82_11760"
     CDS             complement(5037..6107)
                     /locus_tag="VFR82_11760"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU4502318.1"
                     /translation="MAQSVKILSTSFLVLSVVLLLYLVFAIFAPFLTPIMWSLILARL
                     FYPVHERLARFLRGKRALSAILMTLAVMILVVLPVSYVAVLAVNESIHAYQAIRDWIQ
                     AGGMEELPTRLANLPFIGTLSQNVLGNLVLAYNVLRGPSADGSTMFHAIAGTVSGLAM
                     NLAEVATDFLITLFTLFFLFRDSPSLLRTVHKALPIDQNAKTEILGTLDQTVVAVVRG
                     TLLTAVAQGFVAGMTYWLLGISFPLLLGALSGLLSLLPVGGTAFVWGPIVVYLLFNGA
                     IVKAIVLLAVGAGIVGLMDNVLHPYLVGSGVDLPLIVLFFASLGGLAYFGFIGLFLGP
                     IVIGLARATFHIFQQNYQRASA"
     gene            complement(6324..6482)
                     /locus_tag="VFR82_11765"
     CDS             complement(6324..6482)
                     /locus_tag="VFR82_11765"
                     /inference="COORDINATES: protein motif:HMM:NF023180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEU4502319.1"
                     /translation="MTTILFIVLIVLLLGALPAWPYSTNWGYYPSGLLGLVLLVVVIM
                     LLTGRLPA"
     gene            complement(7369..7614)
                     /locus_tag="VFR82_11770"
     CDS             complement(7369..7614)
                     /locus_tag="VFR82_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502320.1"
                     /translation="MLKIAIKKRDPLECSRCDGALLPMRAMYLDTGVIEYLCIKCHRR
                     WLLVDRPTGLVFVSSPQNEKDRLEHAQATGCSTGGQS"
     gene            8630..8715
                     /locus_tag="VFR82_11775"
     tRNA            8630..8715
                     /locus_tag="VFR82_11775"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8663..8665,aa:Cys,seq:gca)
     gene            complement(8734..>11385)
                     /locus_tag="VFR82_11780"
     CDS             complement(8734..>11385)
                     /locus_tag="VFR82_11780"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU4502321.1"
                     /translation="WKKFLAQFHDVLVVLLLIASAISAVLWLYERESPWPYEAMAILV
                     VVLLNAVMGFVQQSRAEEAVAALRRMSAAQARVIREGLHKTVAATDLVPGDILLIEEG
                     DTIPADARLIWSAALQTAEAALTGESLPVSKDPAVLAGETSLGDQHNMVFSGTTATYG
                     HGRAVVTETGMYTQMGRIAGMLKRPQSERTALQKDLDRVGKRLGLIVVVIAALMITTV
                     ILIENVRGLSALFDVFILGVALAVAAVPEGLPAVVTAVLSLGVHRMAKRKAIVRRLAA
                     VETLGSATIIATDKTGTLTKNEMTVRRLITASGQHSFTGTGYAPEGEMRREDRSSAEA
                     PVRFEALRALAAADRANNAVLQDCGGHWVVRGDPTEGALIVAARKAGLDQEEVQERFL
                     RLAEVPFSSTRKLMSTIHTDADKQDRLIAFTKGAPDVLLSRCTHELVGTESRPLSDTR
                     RAEILRNNEELANQALRTLGVAFRSLPKNFEPEAADEGVEQQLVFLGLIGMMDPPREE
                     SSQAVARAKQSGISVIMITGDHPKTAVAIAKELGIIEKGEAITGAQLEQMPDEALERA
                     VQHASVYARVNPEHKLRIVKALQRRDAIVAMTGDGVNDAPALRTADIGVAMGMAGTDV
                     SREAADMVLADDNFATVVAAVEEGRAIFTNIRKFLRYLLSSNVGEVLTMFLGILLADF
                     IGLRQTGGGLVVPLAATQILWLNLVTDGAPALALGVDPPDPGLMTEPPRPRHEEVITG
                     SMWRGIFFIGIIMATGTLFVLDVSLPGGFFEGSGTIRHAQTMAFTTLVLFSLFTVFNA
                     RSDKRSAFKGLFSNLWLWGAVTLSLFLQAAVVYVPFLQQAFSTVSLSPIDWMYCALVA
                     SSVLWLRELSKILIGSEGGLGDCHKRF"
BASE COUNT         2666 a         3069 c         3044 g         2606 t
ORIGIN      
        1 aagtccgctt tcgtgcctaa ctgcttcgcc agacggacct gttctttgat caaatcgatg
       61 cgtgcaccgg gaacaccgcg attaattaag actgttcccg ggatgcgttg ttggagaccg
      121 cggttgatga gatagacgta gccctccgtt aaagggattg cccccacgcc ggtggcgtca
      181 ctggctccaa gcgccatata gacgaattca atttttcttt ccttgggggc tccactacag
      241 ctgaggatgg ccagcgaaca aagggagagg agaattcctt tcatgacatt cctgcacatg
      301 cggatctcct ttcgctcgag caataccggg aataaccctt acgtactcag ctagatcaga
      361 gggtggtaat tccctgggcc agagcaaact tgatgaggtc ggaggtcgta tgaactccca
      421 gtttgtgcgt aatgttggct ttgtggaatt ctaccgtctt cctcgaaata ttgagctgtt
      481 gggcgatgtt cctcgtcgtc ctcccttcgg acagcaaggc taatacctca cgttgccggc
      541 cggtgaggtc gccggaataa ccctcgggcc tgaaccactc gtgttccacc gcctcccgca
      601 cttgaggctc gatggtgttt gacaggaaac gtttatgggc aagcaccgta tcgattgccg
      661 tacgcagttc atccgaggcc gactgcttta agacgtaccc gttcgctcct gcccgaaacg
      721 cctcgctgac ggaaatggct tccgtcaaca tcgtcacgag gataactttc agcttgggaa
      781 ggagcttttt tagttctctg gttgcctcaa aaccgcttaa gcccggcatg caggcatcca
      841 acaggatcac gtcgggacga tgctcgagcg cgctactcac aatgtcgccg gagtgttgca
      901 cggtgctgac gatcatgaaa tgcgggtcta gtagtttcgt aagactttcg aggaccaggg
      961 gatggtcatc gaccagcatc actcgagtgg cctgtctgtc cagaggcatc cttttttcct
     1021 accaggtttg gcccattata gctgtccaac atggctggga tatttatgac cacacgtggg
     1081 tcgtatcctc gccgtaagaa ttaacacacg tccatcttcc agccccatcg tatatcgctc
     1141 cttgaaaatg ggtctgtcct ggtattcata tccggaatct cggccaagga aagcatctat
     1201 cagtccttgc tcttccgccc gccactcttc ggcttccgca atggtaagcg ccttcacggc
     1261 ctccctatga caccatccga tacgagcgat gaggcaggca ttgatatctc cgctggcggt
     1321 cgtgccgatg cttgacccat aactgtgatc taaaacggca tcttcttcat ttaccaaagt
     1381 ggctagcatg gtagtcatgg tgtatcctca caataagtct tgcagttaaa tcaaatcgca
     1441 gctcgaaaaa gacagagcaa gatccgtaaa tgtttaacgg ggtggatctg tttgagatgg
     1501 gctggctgtg tatcgatgat gttcttgagc ggccatgttg gctacaaacc aagcaagctt
     1561 tgagccaacc tcgtttagcg ggcaaaacac tcgcattgtt gcggctatgc accagttttc
     1621 aatcgagacg tctctatctt tgtacatttg tgtttttttg gaccaatgga agtgaaggct
     1681 gtcatcacta ggaggttctc tagggggagg caaatgcagg caaagacaag ggcttttaag
     1741 cgtgtaggaa aagacctgat gcgggtatgg tccgtgaata agactgcaca gcaaccctct
     1801 cggtaaaaat ggaattagac caaacacctt ccaacggatc gcaggccatt cgggtcttgc
     1861 ttgtcgatga tcatgcgatg ctgcggcaag gcctcagaaa cctcctccaa aattatggaa
     1921 atgttgaagt cgttggggag gcggccgatg gagaagaggc cgtaactctg gccacacggc
     1981 tcgatccctc ggttgtcgtc atggatatca acatgccaaa gatgaatggg attgaagcca
     2041 caactcgcat tcatggccaa gcgcctcatc ttccgatcat tgggttgtca gtgaatggca
     2101 tagaacacgg aaatgccatc ctccgggctg gcgccaaggc ctttgtgtcg aaagaagcgg
     2161 ccgtcgatca tctctacgtg gcgatcctca atgcggtgaa ctgtagctga ggaggccgcg
     2221 cgcagaacga gacatgacga gtttgtgaaa cgagcctcga tgagatatcc gttaacaaag
     2281 ggctttacta tccttgatta accttgccgt cagatctttt ttccactcgg ttactcgttt
     2341 cacgaaaagc ttagagggcc gcgttcaccg ttcactgcta gacactgact catcctcgtc
     2401 tctcgtggat ggcaaggtta atgcctatca cctctcatcc gcacctccaa ccatacaatt
     2461 ggtgatactg gactctagtc gcaacccacc ccaccggtca cccttgtagg acagggaatc
     2521 agccgtattg aggcgagaca gctatgcaat gccgcgcggc ataagacaga aatggcaaaa
     2581 acagtttgtc ggagaagagt caacgtatag actacgcctg agactgaagc gagccaccca
     2641 taggagctcc acatgaggaa aaggcgcatc atcgtgaaac ggcgtcctcg atccactgcc
     2701 catgagtggg aacgaggatg ggatttagca atgaccatta ttgatggtgt tccgccactg
     2761 gtggaaaagc agcctatttt tcaggactgc ctgactgtcc tggacggggc gtttgcagat
     2821 ggaaatagtc tgcgatttga gcttggactc agtgccctca ttgacttctg taacgaaacg
     2881 gtgagcagag gcgagtgcac gcagtggtgg agctagatag tggcctgcca gacccgtcaa
     2941 tcgtcctgca tgccattgca aacacttgct cagctttaag cccgcctaga ctattccacg
     3001 tttcgccggt ttccatccag ggcggcggca tctgaaactc ctcacaaacc gtaaagcagg
     3061 acgctgttga agtggcggca gagggagcct agtttgtcct gtgctggtag cactggagta
     3121 gcacatggtc aggtgacggg gatgagagaa ggttatgagg gtatgggacg gagtagtact
     3181 ttctccagta tcgggacaaa ggcaggcttg atccaaacct tctgcctctc gcccatttca
     3241 agaaaagagg tgcggccgtt aacggtggca acgaatttat acccactttg tatgccatgt
     3301 ctggcacagc acacaatgat ctctccctct tccatacttt gtttgatcaa aagttccttc
     3361 agaacctcta tctcctcttc ggtacacccg cgtagtgcgg ccacagcatg ggccatacgg
     3421 gccggcagaa tctcttcctc ctccgtgatc atttcattct caggcccatc gagaaaccgg
     3481 ttcaggtcga agagcccgtt tttgccgaca gtcacttgcg cagaaaggat aaccttgtcc
     3541 ggctcctgcc attttctgat gctgtcatac gggatcttgc cttcatgatc cgtgaacttg
     3601 ttcaagacga caatgccgtc ttgttcgccg tattcctcga caatcaccca aaagttgcga
     3661 gatttggcga tcagcttccc gtccatcatt ggccgaggca gcagcctcac aattctttca
     3721 atgctgtcat gaaagaatcc tcggttcatg aaccgatacg ctatcagagg cgcgggggaa
     3781 agccaattga tcccgcattt cctgaatcgg ggttggggta tgcggcgaga ggtcgagtga
     3841 cgaaagcagc ttcaatgcca atggatctgg gagggtgcct cgacgggtat tggccttttg
     3901 caccggcatg cctttagctc tatactttga tatcctgtcg gcctaggatg acatcgcgag
     3961 ttccgacgaa ctttcggctt cccgtcaggg caagaaaggt ggctatgaac ggacgaattc
     4021 tgctgatcgc aatgctctta ccgggggtcg ctgggtgcga gtcgtattca tggtctgtgt
     4081 acaacaatcc atcaattacg gatatcaaac aaggtcagga ctgtcgagtt ctggtgttcg
     4141 ggcttggtgg taccgttgat ccgaccggaa ttcaagccat gcgtctcggt ggtgtgacaa
     4201 aagttcggag caccgagtat cgcgtcaaca cgattcaagg cgtggggaac gagtgcatca
     4261 tcgctcacgg agagtaaccg acacgccacc tctccttccc ccggtaaagg tgccggggga
     4321 agacctctca gggttctttg cgtctagagg cttcgcgttg gttccgaccc tctagtgctt
     4381 cgccttcaaa tgggcctgca ggatgccgga attctggatc tcaggattgt cttttccata
     4441 ctgctgagcg gccttgaagt gctggatcgc cgcctggtga tctccagatt tatccagcgc
     4501 gagtgccaag tcatagtggg cctcggcgga ctgatcatca gcctgcaccg ccttctcgaa
     4561 atgctttttt gcgacgtccc agtgcccttg ctcataatgc atcatgcctt ccttgatggc
     4621 ggattccgct ttcgagcctg agggagccgg cagcgccggt gcggtcttac ccatatcggc
     4681 ccatgctatg gaggccatca atagcacccc aacacttccg atcgaacaca gcataatacg
     4741 tttgtgacct tgcttcataa gaacctccat agtgaattac gtgattccta acaccggttc
     4801 gctgccccat tgaactgggt gagccccttg ctgcacgaga gtgatgactg aatttggact
     4861 gctctgcatc aatggacacg aagtcggtgc ataataagct tggtgaatgt gtcgctgtgg
     4921 atgaaaacaa aggtggcatg ttctgagccc aattccactc tatcgggaga cagcaaggta
     4981 cgtagggggg aaaaaaccca gtccaattgg aagagctcac tggcaaacaa gtgaggtcag
     5041 gcggatgctc gctgataatt ctgttgaaag atgtgaaacg tggctcgtgc caggccgatg
     5101 acgatcggtc ccaggaaaag accgatgaag ccaaaatagg ccagccctcc aagagaagca
     5161 aaaaagagca cgatgagtgg caggtcaact cctgatccca ccaaatacgg atgaagcaca
     5221 ttatccatca gaccgacaat ccctgccccg accgcgagta atacaatagc tttcacgatc
     5281 gccccgttga acaacaaata gaccacgata ggtccccaga cgaaggccgt cccgcccact
     5341 gggaggagag agaggagtcc gctcagcgca ccaagcagca agggaaagga aattcccagg
     5401 agccaatacg tcattccggc gacgaaacct tgcgccaccg ctgtcagaag ggtgccacga
     5461 actacagcga caacggtctg gtcaagtgtg cccaagatct cagttttggc gttctgatcg
     5521 ataggcagcg ccttgtgaac ggtccgcaag agagagggac tgtcacgaaa caggaaaaac
     5581 agcgtaaaga gcgtaatgag aaagtccgtg gccacttcgg ccagattcat cgccagacca
     5641 ctgactgtgc ccgcgatcgc atggaacatt gtcgatccgt ctgcgcttgg gccacgaaga
     5701 acattatatg caaggacaag gttcccaagg acattttggg aaagcgtgcc gataaacgga
     5761 aggtttgcca gtcgtgtggg caactcttcc attccaccgg cttggatcca atccctgatg
     5821 gcttgatacg cgtggatgct ttcattcaca gccaacacgg ccacgtatga gaccggaagg
     5881 acgactaaaa tcatcacggc taatgtcatg agtatggccg aaagcgccct ctttcctctt
     5941 aagaaacgtg ccaacctctc atggactgga tagaaaagcc tcgccaggat aagagaccac
     6001 ataataggcg tcagaaatgg ggcgaaaata gcaaatacga ggtacaagag aagaacaaca
     6061 gacaggacga ggaaagaagt tgagagaatc tttacagatt gagccatgcg agcaacgtgt
     6121 tggtggaggc ggttggttgt acaggatgga tcaactggcg gtggctcctt tcccaggcag
     6181 gtcctttgcg acgcacttca tctgacaaga cgagtgtcaa acaccctttg tcagtgtgac
     6241 atgggaaata caatatttgc tagccggagc caaactcagt gtatgtcgct gaggctatga
     6301 agccaacagt gacctcccgt ctattatgca ggcagacggc cggtcaaaag catgatcact
     6361 acaacaagaa gcacgagacc caacaacccg ctggggtaat acccccagtt tgtactgtag
     6421 ggccatgccg gcagtgctcc cagaagcagc acgatcagca cgatgaacag aatggttgtc
     6481 ataaaatcct cccgtaaagg ttctccctta atttgagtgt gatccgtgtt ttcacctaat
     6541 tcatactagg actaccccta gacagttccc tgctgtcatt ccttggaggc ccggggtggt
     6601 ggccagttca gcgacgaagc cattcccttt ctagaaatgt acgggctact cgacacatta
     6661 caaccagcac gtgatagagg gagcacgagc gtttgcttga tgtgttcagc aagaaagagg
     6721 caatggaagg actgggactg ggtcgagaca gaaacgccgt gcgacgccgc gtggctcgca
     6781 ctcctttacg tcatccctga ggtgggcaga ctctctgtgg tggtgggaca gacgggttgt
     6841 ctgaaagaag ttgcaaagat agcttcactt acaaagtatg cgtgaaggaa atattacatc
     6901 aggggctcgc tgaaatgtgc cgcttaccag tacgacttcc tgaatgtact aggagccgtc
     6961 tgattccagg gataactaaa tccgtaggcg agtccctgtc tatcctaggg ctttagatag
     7021 gcgacgaacg gacgggttca atctagactg cgcgactgtc gtcttttcag tggattgtcg
     7081 ctcgaatgca gccctcgcaa agaagtcccg ggtggcgccc tgtccttgcg aagggcacaa
     7141 agtacaccac cgcaatcagc ggttcacctc ccgtcgactc ctccaagaag gacagccaga
     7201 tcctgccaca tttctgtctt cctcatgccg ttcgcgtcgg tcgagaaggt ttcgtcgatg
     7261 ctctgtgcag tattttcgga ttgtatccct ggcagtgcac tctttgccat cgctgttttc
     7321 atcgccataa gcgatgatca atcctcaacg cttgatgcgc ttggatagct agctttggcc
     7381 accggtggaa cagcctgtcg cctgggcatg ttcaaggcgg tccttttcat tttgaggtga
     7441 tgagacgaag acaagccctg tcggtcgatc gaccagcagc cagcggcgat ggcacttgat
     7501 gcacagatac tcgatcactc cagtgtccag atacatggcc cgcatgggca acagagcgcc
     7561 gtcgcatctg gaacattcca acggatccct ttttttgatg gcgatcttta gcatgtgatc
     7621 tgccctcaca acgcggtgca ctatggcaga cttgggggcg atccactagt gccggaaggc
     7681 cctagaaaag acatgcttgt gtagagtcga ctcattgctt gaaatctggc ctcagattca
     7741 tcacagacct ccagagctga tcatgaaaag cgtgcgagga ttttatttcg ctccacagcc
     7801 gggatgtgtg caagggcacg tcgctcctgg cgcaggtgcc agggccttct cacattcatc
     7861 actgcaaaat gagctgtgat ctcttgcctg acacttacag gcctgatgcc cacagttctt
     7921 tggatagttg attgtcggcc ttggacgtat attcatctag cccccttact ccctagttta
     7981 cgtgtgtctg atacttttcc cgacgaagtg gttaaaaaag cgcgattagg ttagcttggc
     8041 actacaagca ggttgatgga aaggtaatgg gttgggctta tcccgacaca agcatgttgg
     8101 cgcggttgac agtttaagcc ctgctacgaa tcgaacttcc accccctgag gggatggaaa
     8161 acagatagcg ctggagagta agaaaggtca agaaaggatc gctgctgact cggtacatca
     8221 catattagcg ctaagccgcc aatcttctcc aaaacgaggt aatgagcggc tgatactcgg
     8281 tatggccgca ctgtgggcac tccagcacaa tagtctgctc gttgttagcc tcttcgttct
     8341 ctcgcccggg gagccgttca atcatgaaag cgtggcactg ttcacacatc atggctgcct
     8401 ccctagctaa taacgatccc tcctctgatt aaggcaaact cctcccctct atatcaaagc
     8461 ctcttccttt ttttgccttc agggtaaccg atagcccccc agatcggtaa ctggggaagc
     8521 gtctaagaat agagatcaga taccctaaag cgttctcagg actttttacc ccccttatca
     8581 gggaaatgac cgatttgctt cccgaatgca atgggtagga ttgcagtacg gagaggtgcg
     8641 agagcggcta ttcgaagcgg ccgcaaggcc gctgccccag atgggccgta ggttcgaatc
     8701 ctaccctctc cgccatgcaa cacagacgtg tctctagaat cttttgtggc aatcgcccag
     8761 gccaccttct gaacctatga ggatcttgct cagttcacgt agccacagca cagaactggc
     8821 tacaagggcg cagtacatcc agtctatggg gctcaagctc acagtggaaa aggcctgctg
     8881 aagaaagggg acgtaaacca ccgcggcttg gaggaaaagg gacaaggtaa ctgcgcccca
     8941 cagccataag tttgagaaga ggcccttgaa tgcgcttctc ttgtccgaac gcgcattgaa
     9001 cactgtgaat agagaaaata gcacaagtgt ggtaaaggcc atcgtttgcg catgccgtat
     9061 ggttcccgat ccttcgaaga agcctccggg caggctgaca tcgagtacga acaaggtgcc
     9121 tgtcgccatg atgatgccga tgaagaaaat acctctccac attgaaccgg tgatgacctc
     9181 ttcgtgcctt ggccgaggcg gctcagtcat aaggcctggg tccggagggt ccaccccaag
     9241 cgcgagcgcg ggtgcgccgt cggtgacaag attgagccat aagatctgag ttgctgctaa
     9301 cgggaccacc aggcctcctc ccgtctgcct cagcccaatg aaatccgcga ggagtatccc
     9361 aagaaacatg gtgagcactt ctcccacgtt ggacgacaga aggtagcgca ggaatttgcg
     9421 gatattggtg aagatcgcgc ggccttcttc cacggcagcg acaactgtgg caaagttgtc
     9481 gtccgccagg accatgtccg cggcttctct ggagacatct gtaccggcca ttcccatggc
     9541 taccccgata tcggctgttc gcaatgcggg ggcatcgttg acaccgtcac cagtcatcgc
     9601 cactattgcg tctcgacgtt gtaatgcctt cacgattctg agcttgtgtt cgggattgac
     9661 acgcgcatag accgaagcgt gttgaacggc tcgttcgagg gcttcgtccg gcatctgctc
     9721 cagttgagcg ccagtgatgg cttctccctt ctcaatgatg ccgagctctt tagcaatggc
     9781 gacggcagtt ttcggatgat cccctgtgat catgatcacg cttatccccg actgctttgc
     9841 acgggccaca gcctggctgc tttcctcccg tggcggatcc atcatgccga tcaagccgag
     9901 aaaaacgagc tgctgctcga ccccctcatc agccgcctcc ggttcgaagt ttttcggcag
     9961 ggaccggaat gcaactccta gtgtccgtag cgcctgatta gccaattcct cattgttgcg
    10021 cagaatttct gcgcgacgcg tatcagacag cgggcggctc tccgttccga ccaactcatg
    10081 cgtgcagcgt gaaagcaaga cgtcaggtgc tcctttggta aaggcaatga ggcgatcctg
    10141 tttgtcggcg tccgtgtgaa tcgtgctcat cagcttacga gttgatgaga atggaacttc
    10201 ggcgagacgc aggaagcgct cctgaacctc ctcctgatcc aatccggcct tacgcgcggc
    10261 aacgatcagg gcaccttccg tgggatcacc ccgcacgacc cagtgtcccc cgcaatcttg
    10321 caggacggca ttgttggcgc gatcggcggc tgcaagcgcg cgcagggcct caaagcgtac
    10381 cggcgcttca gcggatgatc ggtcctctcg tcgcatctcg ccttcaggcg catagcctgt
    10441 gccggtaaag gagtgttgtc cactggcggt gatgagcctt cggacagtca tctcattctt
    10501 ggtcaaggtc ccggttttat cggtcgcgat gatcgtcgcc gatccgagcg tctcaactgc
    10561 cgctaaacga cggacaatgg ccttgcgctt ggccatacga tgtacgccaa gagaaaggac
    10621 ggcggttacc acggccggca gaccctccgg cacggctgca acggccagcg cgacgccgag
    10681 aatgaacacg tcgaagagcg ccgaaaggcc tcgcacgttt tcaatcagga tgacggtcgt
    10741 gatcatgagg gcggcgatca caaccacgat gagcccaagc cgcttgccga ctcgatcgag
    10801 atctttttgt aatgcagtgc gttcactctg ggggcgcttt aacatgccgg cgatgcgtcc
    10861 catctgcgta tacattcccg tttccgtcac gacagcacgc ccgtggccat aggttgccgt
    10921 tgtgccactg aacaccatat tgtgctggtc cccgagactg gtttcccccg cgaggaccgc
    10981 aggatctttt gaaacaggaa ggctctcgcc cgtcagcgca gcttccgctg tctgtagtgc
    11041 ggctgaccag atcagtcgag catcggcggg aatcgtgtcg ccttcttcaa tcagcaagat
    11101 gtcacccggc acgaggtcgg tcgcggcaac agttttatgc agtccctccc gaatgaccct
    11161 ggcctgtgcc gccgacatcc tgcgtagcgc cgccacggct tcttcggccc gggattgctg
    11221 gacaaatccc atcacggcat tgagcagcac gacgacaaga atggccatgg cttcataggg
    11281 ccatggcgat tcgcgttcgt acagccacag aactgcggaa atcgccgagg ctatcaataa
    11341 gagaacgacg agcacatcgt gaaattgagc tagaaacttc ttcca
//
LOCUS       DASTQY010000360         4369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_834834, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000360 DASTQY010000000
VERSION     DASTQY010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834834"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VFR82_11785"
     CDS             complement(<1..1062)
                     /locus_tag="VFR82_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU4502322.1"
                     /translation="MAEGNPEDRILILAPIGQDATAMASLLHTHGFLGTVCKSAADAC
                     QYLTAGAGALLVTEEALELPQMPELLQGLNGQPPWSELPMIVLTRGGESRLAELLHLV
                     AQAAGSLTLLERPLGEATLLRSVEVALRARRRQYQVRDLLEQQRIVHKELRESEEKYR
                     SLFESIDQGFCTIEVLFDERDEPVDYRFLSVNPCFERQTGIANAVGRRIREVVPLHED
                     HWFQTYGKIAKTGEAMRFEREAAQLRRHYDVFAWRLGEPAERKVAVLFNDISERKASQ
                     ARLERFTEELERQVSERTQELMSSQGQLRALATELNLAEQRERKRIAAEMHDHLAQML
                     VLVRLKLGQAKQGPAHRSLE"
     gene            complement(1049..2569)
                     /locus_tag="VFR82_11790"
     CDS             complement(1049..2569)
                     /locus_tag="VFR82_11790"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEU4502323.1"
                     /translation="MKQSTVSSPIDERCHTGVEGLDEILAEGLPCNCFYLIQGDPGSG
                     KTTLALQFLLEGVRRGEKALYITLSETKEELYKVAHSHGWSLDGIALFELSAIETLVR
                     PDAHTTVFHPSEIELTKVTDQLLTEIRNTRPSRIVFDSLSEFRLWAETALRYRRQLLN
                     LKQELAKYQSTVLLLDDKMDNRNIAGDPHVLSLTHGVIEMEQLSPDYGRSRRRLRVLK
                     LRGVRFREGFHDYTIEKGGLRVFPRIVAAEHRLEFRREPVESGYKDLDDLLGGGLDRG
                     TTTLIMGQAGTGKSTLALQYAVQMASRGERSILFAFDEVRGLVLARAASLGLRLAPHV
                     ETGVISVQQVDPAELSPGEFAMQIRRAVEEGCKLVVIDSLNGYMNAMPGERYLANQLH
                     ELSAYLNQQGVVTIFILALHGLVSQAEAPIDLSYLADTVVNVRFFEAAGAVRQALSVI
                     KKRSGRHEKTIREFQLESGTGLRVGKPLAEFQGVLSGAPVFHGGSEQMMRQPNGGG"
     gene            complement(2778..3170)
                     /locus_tag="VFR82_11795"
     CDS             complement(2778..3170)
                     /locus_tag="VFR82_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502324.1"
                     /translation="MPYLIDFDATNRILRARCIGRVTDDDLRDMYRFGQENVARFDPL
                     SGITDFSEVTEVAFSPQTMRDLARTKPIMSDPRRLVIFVAPTPDLFGMARMFELEGAE
                     ARPNLRVVHTAEEAYQILNVRDPQFSPF"
     gene            3335..3904
                     /locus_tag="VFR82_11800"
     CDS             3335..3904
                     /locus_tag="VFR82_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU4502325.1"
                     /translation="MAVESIMLPLGTTAPPFFLSDVVNRQVYSLDSFHGKDALLVMFI
                     CQHCPYVKHVEQEIARIGKDYQNKKLGIVGISSNDPATYPEDAPDRLKEMAKRLDFRF
                     PFCFDETQDVAKSYKATCTPDFYLFDAQRRLVYRGQLDDSRPGNNKPVTGHDLRAAID
                     AVLAGKPVDRNQQPSIGCSIKWKPGNAPS"
BASE COUNT          915 a         1359 c         1166 g          929 t
ORIGIN      
        1 ctccagcgag cgatgagcgg gcccttgttt cgcctgaccc agcttcaacc gcacgagcac
       61 caacatctgc gccaagtggt cgtgcatttc ggcagcgatc cgttttcgct cccgctgctc
      121 cgccaggttc aactccgtcg cgagggccct aagttggccc tgagaggaca tcagctcctg
      181 tgtgcgttca gacacctgtc gctccaattc ctccgtaaac cgctccaagc gagcctgtga
      241 ggctttgcgc tcgctgatgt cgttgaagag cacggccact ttccgttcgg ccggctctcc
      301 gaggcgccac gcaaacacgt cgtaatggcg tcggagctgc gccgcctctc gctcgaatcg
      361 catagcctcg cccgttttcg cgatcttccc gtacgtctga aaccagtggt cttcatgcag
      421 tggcaccacc tcgcggatac gccggcccac ggcattcgca atccccgtct gccgctcgaa
      481 gcaggggttg actgaaagga agcggtaatc caccggctca tcacgctcgt cgaataagac
      541 ttcaatggtg cagaacccct gatcgatgga ctcgaacaac gatcggtatt tttcttccga
      601 ttcgcgcaac tccttgtgaa cgatccgctg ctgctccagc aaatcccgca cctgatattg
      661 gcggcgtctc gctcgcaagg ccacttccac tgaacgcagc aaagtggcct ctccaagtgg
      721 ccgctcgagc aaggtgaggc tgcccgcggc ttgggccacg agatgtagca gttcggccaa
      781 ccgcgattcg ccgccgcgcg tcagtacgat catcggcaat tccgaccaag gcggctgtcc
      841 attcagccct tggagcagct cgggcatttg cggcaattcc agcgcttcct ccgtgaccag
      901 caaggcgccc gcgcccgctg tcagatattg acaggcgtca gcagcgctct tacacactgt
      961 tcccagaaat ccatgggtgt gcaacaggct cgccatggca gtcgcgtcct gcccgatcgg
     1021 cgcgagaatc agaatgcgat cttccggatt accctccgcc attaggctgc ctcatcatct
     1081 gttcagagcc gccgtgaaac accggcgcac cgctgaggac accctgaaat tctgccagtg
     1141 gcttgccgac acgcaagcca gtgcccgatt ccaattggaa ttcacgaatc gtcttctcat
     1201 gacgaccgct ccgcttcttg atcaccgaga gcgcctgcct gaccgcacca gcggcttcga
     1261 aaaagcgcac gttgaccacc gtatcggcga ggtaactgag gtctatcggg gcctcagcct
     1321 gactcaccag cccatgaagc gccaggatga aaatcgtcac gacgccctgc tggttgagat
     1381 acgccgacaa ttcgtgtaac tggttggcca ggtagcgctc gcctggcatc gcgttcatat
     1441 atccattcag actatcgatc acgaccagct tgcaaccctc ttccactgcc cgcctgatct
     1501 gcatggcaaa ttctcccgga gagagttcgg cgggatccac ttgctgcaca ctgatgacgc
     1561 cggtttcgac atgcggcgca agcctcagac ccagtgaagc ggctctggcc agcaccaggc
     1621 cccgcacttc atcgaacgcg aacagaatgc ttcgttcccc gcgcgaggcc atctgaaccg
     1681 catattgcag tgccagcgtt gattttcccg tgccggcctg gcccataatc aacgtcgtcg
     1741 tcccgcggtc cagaccgccg ccgagcaagt cgtccagatc cttgtacccg ctctcaaccg
     1801 gttccctgcg aaactccaag cgatgctccg ccgcgacgat gcggggaaag acgcgcaagc
     1861 cgcctttctc aatagtgtag tcgtgaaacc cttcccggaa tcggacgccg cgcagcttca
     1921 acacacgcaa ccgccggcgc gaccgaccgt aatccggaga cagttgttcc atttcgatga
     1981 cgccatgcgt cagactcaac acatggggat cgcccgcgat attcctgttg tccatcttgt
     2041 cgtccaacag caggaccgtg ctctggtact tggcaagctc ttgtttcaga ttcaacagct
     2101 gtcgccgata gcgcaacgcc gtttcggccc acagcctgaa ctccgaaagc gaatcgaaca
     2161 cgatccgcga agggcgggtg ttgcggattt cggtcagtaa ttgatcggtc accttggtca
     2221 actcgatttc ggagggatgg aacaccgtcg tgtgcgcgtc aggacgaacg agtgtttcga
     2281 tcgcggacaa ttcaaacaat gcgatgccgt cgagcgacca cccgtgtgag tgcgcgactt
     2341 tatacaactc ttctttcgtt tccgagagcg taatgtacag ggccttttct cctcggcgta
     2401 ctccctcaag aaggaactgc aaggccaggg tggtcttccc cgagcctggg tcgccctgaa
     2461 tcagatagaa gcaattgcag ggcaatccct cagccaatat ttcatcaagt ccttccacgc
     2521 cggtgtggca ccgttcgtcg atgggagagg agacggtcga ttgtttcata ctcgtccacc
     2581 agcctttctg gctttacctg agtcagatca aaagtatgag gtgttgcttt cagaggccga
     2641 tgggacctat tcataccgcg actctgtagg agccttgtcc taggggaacc cctagctttt
     2701 agggttgcag gaatgaaatc ggaagtccgt ataattagtg gcgcgcaggt caaggcctct
     2761 ttgccaccag gaaaaggtca gaaaggcgag aactgagggt cccggacgtt cagaatctga
     2821 taagcttcct cggccgtatg cacaacacgc aggttcggac gagcctctgc accctctaac
     2881 tcgaacattc gcgccatccc aaaaaggtca ggtgtcggcg ccacgaagat cacaagtcgg
     2941 cggggatcgg acataattgg cttggtccgg gcgaggtcgc gcatcgtctg cggagagaac
     3001 gccacctccg tcacctctga gaaatcggtg atgcccgaga gcgggtcgaa ccgggcgacg
     3061 ttctcttggc cgaatcgata catgtccctc aggtcgtcat ccgtcacccg ccctatacac
     3121 cgcgctcgaa ggatccgatt ggttgcatcg aagtcgatca gatagggcac ttggctcctt
     3181 ggacgtgcgt ttctagttgc cagcgagcaa gactggcgag acaatagccg acgtgggccc
     3241 gtacagtacc atgtttacgg gtgaaccgct gtacgaataa cggactcagc tactgtccta
     3301 cgtaagtgga tcatcgcgac gcaacgagga ctccatggcc gttgaatcca tcatgcttcc
     3361 gctcggaaca accgctccgc cgtttttctt gtccgacgtg gtaaaccggc aggtctattc
     3421 gctcgactcg tttcatggca aggatgcctt gctagtcatg ttcatctgcc agcactgtcc
     3481 ctatgtgaag cacgtcgagc aggaaatcgc ccgcattgga aaggactatc agaataaaaa
     3541 gcttggcatc gtgggcatca gcagcaatga cccggcgacg tatcccgagg acgctcccga
     3601 ccggctgaaa gagatggcca aacggctcga ctttcgtttc ccgttttgct ttgacgagac
     3661 gcaggacgtt gccaagagtt acaaggccac ctgcacgcct gacttttatc tctttgatgc
     3721 gcagcgtcga ttggtctatc gcgggcagct cgacgacagc cggccgggca acaacaagcc
     3781 ggtgacaggc cacgatctgc gtgccgcgat cgacgccgtg ctcgccggca agccggtgga
     3841 ccgcaatcaa caacccagca ttggctgctc catcaagtgg aagcccggca acgccccttc
     3901 ataaaaatct tttcatcact cgctgcgcgc tgctctatcc atgatccgtc taaatttgcc
     3961 cgttcctctt tctccagggg cggatgaaag agttgctgac ctgcgagcgg tgcaggggcc
     4021 tatcgctgcg gtcgccttgg gcgatggtat ttacgatgcg ccctcagagg ttgctgattc
     4081 tcttttttct tagaacagca cgcataacgg aactgtgttc ggccttggct aattgaactc
     4141 atgcttcagg gatggcctgg attggttcca tcgtgcgcgc gtcgaaggag cacctcccgc
     4201 aatctccaat tacgatggat cccgcgtgcc gccttccgcg agcacaggaa ccgatcaggc
     4261 cactgcccta cctcaccact ttgctttttc ccctattcat atataatgcg gccgttcatc
     4321 gcgacgcacc tcaaacgagg agaacaagca tgaaagacac aagccgtgc
//
LOCUS       DASTQY010000361         8631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1483642, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000361 DASTQY010000000
VERSION     DASTQY010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483642"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFR82_11805"
     CDS             <1..294
                     /locus_tag="VFR82_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU4502326.1"
                     /translation="DDFPKFQNANAELFGISCDSFFSHKAWADSLDLKHRLLSDVHRT
                     TAKAYGLYFEPLNCSKRATVIVDKNGKVAYVKVQEIKVAREDKDILSALSKLN"
     gene            366..698
                     /locus_tag="VFR82_11810"
     CDS             366..698
                     /locus_tag="VFR82_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU4502327.1"
                     /translation="MSGIVEDVNDGNYKEFTDSGGAVVAYGLATCEPCKTYDPILEDT
                     AARFPAIRVGKAKMHVPGRCREIKKTHTFETYPTTHFFANGKLLLTREGVVDQAELAA
                     LISDYLLK"
     gene            complement(761..1240)
                     /locus_tag="VFR82_11815"
     CDS             complement(761..1240)
                     /locus_tag="VFR82_11815"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU4502328.1"
                     /translation="MIRLCWVVIGSVMWVSTVQAGADEDAVERVLLAEAKSSATFAET
                     RDTQAVLKLYTNDYKGVQDGEAETRESIEKWLSEYGSALDQGSRVHFLGAVSNVSTHM
                     AGSMAWATYDYVFQAIRNGELDAQERGKCTSVLRKESSGWLIQHEHCSKTKPQGMER"
     gene            complement(1262..2017)
                     /locus_tag="VFR82_11820"
     CDS             complement(1262..2017)
                     /locus_tag="VFR82_11820"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEU4502329.1"
                     /translation="MPPVLLTKRIEFAAAHRYYKPEWDEAKNRAVFGRCFNPPAHGHN
                     YMLEVTVSGEVDPHTGMVINLFDLKRVLLAVLEEFDHKNLNLDMPYFESQIPTSENLA
                     RVLWAKLVTQQDIGVLHAIKLHEDEDLYAEVTAAAGLDVASVTRRYPFVAGNGHLEGR
                     EWDCFVTLSGPVDPVTGMVTDLGALDCLVQDAVVKKYDRQDLRTLFGDGRLVPGDTLV
                     KEIWHALQGGTKSGVLSGVRLVESRDLFYECSG"
     gene            complement(2046..2630)
                     /locus_tag="VFR82_11825"
     CDS             complement(2046..2630)
                     /locus_tag="VFR82_11825"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEU4502330.1"
                     /translation="MKPLTREDLLSLQDYERQRDSFRSRIIALKQRRRLSLGPLISVV
                     FENRDTLRFQVQEMMRAEHIQDPAKVQEELSVYNDLLPAEHELSATLFVEITDEATMK
                     EWLDRFMGLDRGHTVAINAAGETVYGQFESGHSHETKISAVHFVRFHPGREMTRAFAD
                     LHQPVCLMVDHNGYRETVAVPGSLREEWLADLTS"
     gene            complement(2627..3895)
                     /locus_tag="VFR82_11830"
     CDS             complement(2627..3895)
                     /locus_tag="VFR82_11830"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEU4502331.1"
                     /translation="MKGMTLVSSVDAAQLQKETLRIYEICDGCRRCFNLCPSFSTLLN
                     GIDKQEGDVAKLTADDHSRIVDECYYCKLCFNHCPYTPPHQYEIDFPHLMIARKKQLA
                     LERGVSWRDRWLNRTDLVGTIGSWTAPLSNWLIRSPIVRRLAEVIGGIHRDRQVLHFS
                     RETFPRWFAKRTKPARTSAKKVALFSTCLVNYQVTDVGKATVQVLEKNGVEVVVPEQR
                     CCGMPGFDTGDINAIQRAIDANVTSLHSWVAQGYDVVVPAASCSLMLKREYPELRPDE
                     PTKEVARNTFDVCEYLMKMKKDGQLSMDFVKKPGRVAYQIPCHLRDQNLGFKSKELME
                     CAGAQVELIEKCSGHDGAWSAKSEFFDLSMKIAEKAIRTIRQQPADVIASDCPLAGLQ
                     LDQAGAAAHVGGKASLHPIQIVRDAYGLGQ"
     gene            complement(3977..4402)
                     /locus_tag="VFR82_11835"
     CDS             complement(3977..4402)
                     /locus_tag="VFR82_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEU4502332.1"
                     /translation="MSKSLKGTKSHENLKGAFAGESQANRRYLYFARRADIEGYPDVG
                     GLFRDTSEAETGHAFGHLDFLKEVGDPATGVPIGNTEANLKSAIEGETYEYTQMYPGM
                     AKTARDEGFQELAEWFETLAKAERSHANRFQKGLDSLKG"
     gene            complement(4492..5055)
                     /gene="rfbC"
                     /locus_tag="VFR82_11840"
     CDS             complement(4492..5055)
                     /gene="rfbC"
                     /locus_tag="VFR82_11840"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEU4502333.1"
                     /translation="MKVTPLDIPGALLIEPSLFRDRRGLFCETFHAQRYVEAGLTDQF
                     VQDNFSRSVRGTLRGLHYQQPHAQGKLVMAVEGTIYDVVVDIRKGSPTYGTWQGFELS
                     SDNYRQLYIPPGCAHGFCVTSEEAAVLYKCTDFYSPSDERGVIWNDPALRISWPVSMP
                     LLSPKDQAYPSLATTEHELPVYSPSPR"
     gene            complement(5072..5494)
                     /locus_tag="VFR82_11845"
     CDS             complement(5072..5494)
                     /locus_tag="VFR82_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502334.1"
                     /translation="MPQLDVHNPGMPDLQFVLFVSALCTSNLEAINLPKDLRERIFDR
                     CWALINTDAPPVDPKQRVLDLREGTDLTLEACVSTIRSLLSEAGITKLVWDHPVSNPT
                     RDSSTAAQPLIDRLGQLYPADPDIVDPPPSRSDGRATG"
     gene            5561..5836
                     /locus_tag="VFR82_11850"
     CDS             5561..5836
                     /locus_tag="VFR82_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU4502335.1"
                     /translation="MVTISLMGQLQTADGERDVACELASPMTVRQVIQRQGVQLRHLL
                     QLIREKKVLVTINKKIASDDSLVHDGDAIRLVGHDGFGGSGLGPTHS"
     gene            complement(5972..6259)
                     /locus_tag="VFR82_11855"
     CDS             complement(5972..6259)
                     /locus_tag="VFR82_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU4502336.1"
                     /translation="MAKSMTKSQIADHVAQKAGLTKKAAVQLLDDFAALAYREAKNVF
                     VVPGIGKLVLANRKARMGRNPQTGEAIKIPAKRVVKFRVSKAAKDAILGKK"
     gene            6551..7111
                     /locus_tag="VFR82_11860"
     CDS             6551..7111
                     /locus_tag="VFR82_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502337.1"
                     /translation="MGKQLPILPATAQPAETAAEKFIYSRVFCQKENPSPLRLMIDFL
                     KSRGQLPILPPDMNDEALDEWAWVHVALQYSRARKPVQVFCFRDRGTFQEAFEQEKGQ
                     FLEALTSYDDVEASLVADTIKKARFILLTRHDPADITEEGYDFNGWILEFYQENCDGI
                     VQIDGQGFYSPKGSLIVDMSAPSPEE"
     gene            7117..7716
                     /locus_tag="VFR82_11865"
     CDS             7117..7716
                     /locus_tag="VFR82_11865"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEU4502338.1"
                     /translation="MPAQSRSHLARLARKTDEWFHRASAALLGQVPCRMGCSQCCLGL
                     FPVTRLDADLLHEGLAQLPVDKRTLIQQRASAQVSALETAYPNLLASSALDGWSDGEI
                     DHAVSRFDQDRCPALSDDGLCSLYAYRPLTCRSMGIPTQAGQMVEGACRVQTFVPIVR
                     LSESLRAEEQELASLESAALTVHGTSAQGEEILLPYAFL"
     gene            7792..8211
                     /locus_tag="VFR82_11870"
     CDS             7792..8211
                     /locus_tag="VFR82_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502339.1"
                     /translation="MHSILVVDDDEDIRNALQLVLRKAGYRPLLAGSGQQAINLMKGE
                     SLTSSVAVVLCDLEMPGMDGSAVIAHFRAHYPLIPIIVLSGATPNQFLDAVGQQGVGD
                     WIRKPATNEAVLEKVRGAVHLYEIRQADRRAKNLPPP"
     gene            8280..8356
                     /locus_tag="VFR82_11875"
     tRNA            8280..8356
                     /locus_tag="VFR82_11875"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8314..8316,aa:Arg,seq:ccg)
     gene            8416..8488
                     /locus_tag="VFR82_11880"
     tRNA            8416..8488
                     /locus_tag="VFR82_11880"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8449..8451,aa:Lys,seq:ctt)
BASE COUNT         1911 a         2488 c         2333 g         1899 t
ORIGIN      
        1 gatgatttcc cgaagtttca aaacgcgaac gcggaattgt tcggcattag ctgtgacagc
       61 tttttctccc acaaggcatg ggcggattct ttggacctca agcaccgcct gctgtcagac
      121 gtccaccgga ccaccgccaa ggcctatggg ctgtatttcg agccgctcaa ctgctcaaag
      181 cgcgcgacgg ttattgtgga caagaatggc aaggtcgcct acgtgaaggt gcaggagatt
      241 aaggtggcgc gggaagacaa agatattctt tccgcgctct caaaactcaa ctaggagcac
      301 ggctatgact gagggctgcc gatcgacacc agtggcagtc ctcagcatcg agccgtacca
      361 ctcgtatgtc aggcattgtc gaagacgtca acgacgggaa ttacaaagaa tttacagata
      421 gcggtggagc ggtggtggcc tatgggttgg cgacctgcga gccgtgcaag acctacgatc
      481 ccattctcga ggacacggcc gccaggttcc cggcaatcag ggttggaaag gcgaagatgc
      541 atgtccccgg ccgctgtcgg gaaatcaaaa agacccacac gtttgaaacc tatcctacga
      601 cgcatttttt cgccaacggg aagctgcttc tcacccggga aggcgtcgtc gatcaggcag
      661 aactggctgc gctgatttct gattatctcc tcaagtagcg ctcataacgt cgagcgcccg
      721 ccgggctctc accggaagta agccaactcg ccgacatccg ttaccgttcc atcccctgcg
      781 gcttcgtttt ggagcaatgt tcgtgttgga tgagccagcc cgaagactct ttccgtaaaa
      841 cgcttgtaca tttgccccgc tcttgcgcgt ctagctcgcc gttgcgaatg gcttggaaca
      901 cgtagtcata ggttgcccag gccatggagc cggccatatg agtgctgaca ttggagaccg
      961 ccccaagaaa atgcaccctg cttccttgat caagtgcgga tccatattca gacaaccact
     1021 tctcgatgga ctctcgggtt tcggcctccc cgtcttggac acctttgtag tcgtttgtgt
     1081 agagcttgag cacggcctgt gtatctctgg tttcggcaaa cgtcgcagat gattttgcct
     1141 cggcaagcag cacgcgctcg acagcatctt catcagcccc cgcctgcacg gtcgagaccc
     1201 acatcacact tccgatgacg acccaacaga ggcgtatcat gaaatcctcc gaaacatggg
     1261 gctaccccga gcactcataa aacaggtcgc gagactccac aagccgaacc ccggacagaa
     1321 caccggattt tgtgcccccc tgcagcgcgt gccagatctc tttgacgagt gtgtctccag
     1381 gaacaagcct gccatcacca aagagggttc gtaaatcctg acggtcatac ttcttcacga
     1441 ccgcatcctg caccaagcag tcgagcgcac cgagatcggt gaccatacct gtcacagggt
     1501 ctacgggtcc gctcagggtg acaaagcaat cccactcccg accctcaagg tgtccgttgc
     1561 ccgcgacgaa tggatagcgc cgcgtcacgc tggccacatc gagtcccgcg gccgccgtca
     1621 cctccgcata cagatcctcg tcctcatgca atttgatggc atgaagcact ccgatatctt
     1681 gttgtgtgac gagcttggcc cacaatacgc gggcgaggtt ttccgatgtg ggaatctgcg
     1741 actcaaagta cggcatgtcg agattcaaat tcttgtggtc aaattcttcg aggactgcca
     1801 gcaagacgcg cttcagatca aataaattga tgaccatgcc cgtgtgggga tccacctcgc
     1861 ctgaaaccgt tacctcgagc atgtagttgt gcccgtgggc aggcggattg aaacaccgtc
     1921 cgaacacggc acggtttttg gcctcatccc attccggctt gtagtaccgg tgggccgcgg
     1981 caaactcaat acgtttggta agtagaacgg gcggcataca cccctatggc gaccaaatgc
     2041 gctgcttagc tagtcaggtc tgccagccac tcctcacgaa ggctgccagg aacggccacc
     2101 gtttcccggt atccattgtg gtcaaccatc aggcacacag gctgatggag atcggcaaac
     2161 gctcttgtca tctcccggcc ggggtggaat cgcacgaaat gcacggcgct gattttggtc
     2221 tcgtgactgt gaccgctttc aaattgcccg tacactgtct cgcctgcagc gttgatggca
     2281 accgtgtggc cgcggtccaa tcccatgaat cgatcgagcc actccttcat agtggcctcg
     2341 tcggtaatct caacaaagag ggttgcgctc agctcgtgtt ccgccggtag cagatcatta
     2401 tagacgctca actcctcctg cactttcgca gggtcttgaa tatgctcggc tcgcatcatt
     2461 tcctgaacct ggaaccgcaa cgtgtcccga ttctcgaaca ccacgctgat gagcggcccg
     2521 agagacagcc gtcgccgttg tttcagggcg atgattcggg aacgaaatga atcgcgctga
     2581 cgctcatagt cctgcagcga caacagatct tctcgagtca acggtttcat tgacccagcc
     2641 cataggcatc acggacgatt tgaatgggat gaagagaggc cttcccgccc acgtgggccg
     2701 cggcgccggc ttgatccagc tgtaagcctg cgagcggaca gtcggacgcg atcacgtcgg
     2761 ccggctgctg ccggatggtg cgtatggctt tctcggcgat cttcatcgac aggtcgaaaa
     2821 actcggattt ggcagaccat gccccgtcgt gtccggagca cttttcgatg agctcgacct
     2881 gagctccggc acattccatc agttccttgg acttgaaccc gaggttttga tcacgaagat
     2941 gacaagggat ttgatatgcg acacggcccg gcttcttcac gaaatccatc gagagctgac
     3001 cgtctttttt catcttcatc aggtattcac agacatcgaa ggtgtttcgg gccacttcct
     3061 tcgtcggctc atcgggccgc agctccggat attcccgttt cagcatcagg ctacaactgg
     3121 cggccggcac cactacatca tacccttggg cgacccacga gtgcagcgag gttacatttg
     3181 catcgatggc ccgttgaatc gcattgatgt ctccggtatc aaacccgggc atgccgcaac
     3241 agcgctgttc tggaacgacc acctcgacgc cgtttttttc caacacctgc acggtagcct
     3301 tgcccacatc tgtcacctgg tagttcacga gacaggtaga aaacaatgcc actttcttcg
     3361 cagacgtgcg ggcgggcttc gtacgcttgg cgaaccagcg cggaaacgtt tcacgggaga
     3421 aatgcagcac ctgccggtct cgatgaatac cgcctatcac ttcggccagc cgccggacga
     3481 tagggctccg tatgagccaa ttgctgagcg gagctgtcca gctgccaatg gtacccacga
     3541 gatcggttcg attcagccac cggtcccgcc agctcacgcc gcgctcaagc gcaagttgct
     3601 tttttctggc aatcatcagg tgcggaaagt cgatttcgta ctgatgaggc ggcgtgtaag
     3661 ggcagtgatt aaagcagagc ttgcagtaat agcactcgtc gacgattcgg ctatggtcgt
     3721 ctgccgtcaa cttcgctacg tccccctcct gcttgtcgat gccattgagt aacgtactga
     3781 acgacgggca aaggttgaag caccgacgac atccgtcgca aatctcataa atgcgaaggg
     3841 tctctttctg cagctgcgcg gcgtccaccg aactgacgag agtcatccct ttcatctttt
     3901 cccaaccgct ccctcaaaca cgaagggtgg actaaatcgg attcagtcca cccctctgct
     3961 ccgcccgttg ccgctctcag cctttcaaac tgtccagccc tttctgaaaa cggtttgcat
     4021 gggatcgctc tgccttcgcc aacgtctcga accattctgc cagctcctga aagccttcgt
     4081 cgcgcgccgt tttggccatt ccaggataca tttgggtata ttcataggtc tcgccttcga
     4141 tggcagactt aagattggct tccgtattgc caatcggcac tccggttgcc ggatcgccca
     4201 cttccttcaa aaagtccaaa tggccgaagg catgaccggt ttccgcctcg gaggtatcgc
     4261 gaaacagacc acccacatcg ggataccctt cgatgtccgc ccgtcgagcg aaatagaggt
     4321 agcgtcggtt cgcctgcgac tcaccggcaa aggcgccctt cagattttca tggctcttcg
     4381 ttcccttcaa gctcttgctc atcgaatcct cctcgttaaa tccctgacta tcactctcag
     4441 ggatgtccac cattgtaggg gctataagat agcccagcga ttagttcagg atcaacgggg
     4501 tgatggactg tacaccggca actcatgctc tgtcgtcgcc aaactaggat aggcttggtc
     4561 tttaggggaa agcaacggca tggacaccgg ccacgaaatg cggagggccg gatcgttcca
     4621 gatcacgccc cgttcgtcgc tcggcgaata gaagtcggtg cacttgtaga gaacggccgc
     4681 ttcctcgctg gtcacgcaga aaccatgcgc acagcctgga gggatgtaca actgtcggta
     4741 gttgtccgag gacagctcga acccttgcca ggttccatac gtgggagaac ccttccgaat
     4801 atcgacgacg acatcataga tcgtgccttc gaccgccatc accaattttc cttgggcatg
     4861 cggctgttga tagtgaaggc cacgcaacgt cccccgcact gagcgagaaa agttatcttg
     4921 aacgaactgg tcggtgagac cggcttccac ataccgttgc gcatgaaatg tctcgcagaa
     4981 tagtccccgt cgatcgcgaa acaaactggg ttcgatcagt agtgcaccgg gaatatcgag
     5041 cggagtgacc ttcacggtgt cagtcctgga gtcatcccgt cgcacgacca tccgaccgag
     5101 atggcggggg atcgacgata tccggatcgg cggggtacaa ttgaccgagt cgatcgatca
     5161 acggctgagc cgctgttgag ctgtccctcg tcggattgct caccggatga tcccagacca
     5221 gttttgtaat gccggcctca gacaagagtg atcgaatcgt ggagacacag gcctcgagcg
     5281 tgagatcggt tccctccctg aggtcgagaa cccgttgttt cggatcgacg ggtggcgcat
     5341 cggtgttgat caatgcccag cagcggtcga aaattctctc gcggaggtct ttcggcagat
     5401 tgatggcttc aagattcgag gtgcagagcg ctgacacgaa gagcacgaac tgcagatcgg
     5461 gcatccccgg attatggaca tccaactgcg gcatggcctg gcattatcag gtggcgttca
     5521 gagggagtca accgggttta acggagagtt ggtaagcaat atggtgacca tatcccttat
     5581 gggacaactc caaacggccg acggtgagcg agacgtggcc tgtgaattgg cttcgccaat
     5641 gacggtgcgg caagtgatcc aacgccaggg tgtacagctc cggcacttgc tccaactcat
     5701 tcgcgaaaaa aaggtattgg tcacgatcaa caaaaaaatc gccagcgatg actcgttggt
     5761 gcatgacggc gatgcgatcc gactcgtcgg ccatgacggg ttcggcggga gcggcctggg
     5821 gccaacccat tcctgactcg gtgagagcgg gtcagttcac aaaaggtctt gcgtttcaac
     5881 aatgagaaag gccggcgccc tagagggagc cggcccttcg tctatagagg cgcaaggcgg
     5941 aaagctgatg agcttcgcgc ccgatggttg gttacttctt accgagaatg gcgtctttgg
     6001 cggccttcga cacgcggaac ttgaccaccc gcttagccgg aatcttgatc gcttcccccg
     6061 tctggggatt gcgtcccatt cgtgccttgc gattggcgag cacaagctta ccgatcccgg
     6121 gcaccacaaa aacattcttc gcttcccgat atgccaaggc ggcaaagtcg tccagcaatt
     6181 ggacggccgc tttcttggtc agtccggcct tctgagccac atgatcggct atctgcgact
     6241 tcgtcattga tttagccata cgagaaacct cccttaaaaa cagaattagg tgaattgcgg
     6301 gctgctgcct ctcagtggag acttcgtgcg atgaagtgac tcgtcaaaga gaccgtgatg
     6361 gatgtttcca gccaaaaacc tgcgggagtc tatccaagcg ttcttggcct gtcaaccgaa
     6421 aaacacgctc caggcgtagg gaaacgcctc cacggaggcc ttgcagtttc aagaccaagc
     6481 atcgtagagt aatggcacgc tgcggccgaa atccggtgtt gaacagcatc ggttattgag
     6541 gatttattgc atgggtaagc aacttcccat tctgccggcg acggcacaac ccgctgaaac
     6601 cgctgctgaa aaatttatct attcccgggt gttctgccaa aaggagaatc cctctcctct
     6661 ccgcctgatg atcgatttcc taaaatcccg tggtcagctc cctattctgc ctcccgatat
     6721 gaacgacgaa gcactggacg aatgggcatg ggtccacgtt gcactgcaat attctcgagc
     6781 aagaaaaccc gtacaggtgt tctgcttccg ggaccggggg acctttcagg aggcgttcga
     6841 gcaggaaaaa ggccagtttc tggaggccct cacctcctat gacgatgtag aagcttcgct
     6901 ggttgcagac acgatcaaga aagcacgttt catcctactg acccggcacg acccggccga
     6961 catcacggaa gaagggtacg acttcaacgg gtggatcctg gagttttatc aggaaaactg
     7021 cgacggaatc gtccagatcg acgggcaagg tttttactct ccaaagggct cgttgatcgt
     7081 tgatatgtct gccccatcgc cagaagaata gggtccatgc cagcccagag tcgttcccac
     7141 ctcgccagac tcgcgagaaa aaccgatgag tggtttcatc gggccagcgc cgcgttgctg
     7201 ggccaagtgc cctgtcgcat gggctgttct cagtgttgtc tcggcctctt ccctgtgaca
     7261 cgcctggacg ccgatctcct tcacgaagga ctcgctcaat tgccagtaga caaacggacg
     7321 ctcattcaac aaagagcaag tgcccaggtg tccgctctgg agaccgccta tccaaactta
     7381 ttggcctcga gcgctctcga tggctggtcc gatggagaaa ttgatcacgc cgtcagccgc
     7441 tttgatcagg atcgttgtcc cgccctaagc gatgacggcc tatgctcgct ttatgcttac
     7501 cgtccgctca cttgtcgttc gatgggaata ccaactcagg caggacagat ggtcgagggt
     7561 gcttgccgcg tgcaaacatt cgtcccgatc gttcgtctct cagagtcact gcgcgcagaa
     7621 gagcaagagc tggcgagctt ggaatcagca gcgctgactg tacatggaac atccgcgcaa
     7681 ggcgaggaaa tcctcctccc ttatgcgttc ctctgatacc cacaccctac ccgactacta
     7741 gcattttgcg tatcaacgcg ataccctctg gcgtaaaccg gagtaggatt cgtgcacagc
     7801 attctcgttg tcgatgatga tgaggacatt cgaaacgcgt tacagttggt gctgaggaaa
     7861 gccggctacc gccccttgtt ggccgggagc gggcaacaag ccatcaatct catgaagggg
     7921 gaaagcctta cctcgagcgt cgccgtagtt ttgtgcgacc tggagatgcc cggcatggac
     7981 ggatcggcgg tcatcgcgca ctttcgtgcg cattacccgc tgatccccat catcgtgctg
     8041 agcggtgcca ctccgaacca gttcctggac gcagtcggac agcaaggtgt gggtgactgg
     8101 atccgaaaac cggcgacgaa tgaggcggtc ttggaaaagg tacgaggggc agtgcatctt
     8161 tacgaaatac gtcaggctga cagaagagcg aaaaaccttc cccctcctta gcctctgcta
     8221 gatcaggccg gtggacagag cgtaaaatgc tgtgctatgg tgacggtcct tgtagcggag
     8281 cgcctgtagc tcagctggat agagcatcag cctccggagc tgagggccac aggttcaaat
     8341 cctgtcaggc gcaccaacag cacacactca aggaagttga cgtccgttag tcccctccat
     8401 gcatgcacac ccggtgggcc gttagctcag ttggtagagc agctgactct taatcagcgg
     8461 gccgtaggtt cgacccctac acggcccact aattaatcaa agacttacag aagtgggttg
     8521 gtgggatgat tgggttggtg ttattagcgt gttagaaaga tacacgttca gccttcctgc
     8581 tgccttttta aatcctcctc agagaccgaa ttatagcggc ggtgcatctt c
//
LOCUS       DASTQY010000362         4124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1543144, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000362 DASTQY010000000
VERSION     DASTQY010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543144"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="VFR82_11885"
     CDS             <1..1456
                     /locus_tag="VFR82_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU4502340.1"
                     /translation="TYPEPFYGHGTWSSTYLSKLNIETNKVSSAGARLPRGNYWKFVG
                     DGRGSAILAAGLEDGMAPKAWIAAARVSNSAPWIRLSRLAPHATGLRFEPVALAAGTT
                     DAFMLMDHQGRSALWRVDLSDQRDPILELWHKEFNVHDVLRDGDGALMGVRLATSAAG
                     PLYLDGQARKLDALLRAKWPDRRNEFLDATANLKEVVVRTWGDGSVPTYHVFSGGTES
                     TKLDFVGSSAPEMAKLSLVPSRSIMVSTDTGRHIFAQLTLPATSDEHKRPLVVLVSDW
                     DEDGLVFDPLAQLLASRGYAVVRTQAANSSADESWELAPLVDWDGKVYTDMLTVIRSL
                     LADDRLDAGRACLIGTGYGGYVAMLGNIRGDVQPACAIGINTVTDLASALANARNSGL
                     RRVVERPGRLDLLAKGADPIRKHAASAHGPTLLVNDTLIKEAKAFAAALSKENKNQKL
                     IITDLIGDGFVAQTNREIVAFLDEHLPIAERSGE"
     gene            complement(1500..3047)
                     /locus_tag="VFR82_11890"
     CDS             complement(1500..3047)
                     /locus_tag="VFR82_11890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4502341.1"
                     /translation="MKTLTMLRRREDEVLDAPLKRLDAATAQSSGRLVDEVNTASANI
                     RYEILLPVNIALAGRLRSMWHYDTQNAMRDARVFQAMNVFGRPGEPSMDTKSGNELPR
                     QHPSKGEEPEALRKLAESLAHERTVLRTMIDLIPAFIYAKDAHSRFTACNALVAKRLG
                     TIPSEMIGKTDFDFFPREMAEKFFAEEQALIASGQPLIDLEEVAFDKVRGENRVILTS
                     KVPLKDDNGRLTGIVGTGYDITERKAVDQKLMAMDRHESIGRLAAGLAHEINTPIQYL
                     SDSVRFIDEGVHELLEYLENPAGGAERPSDVEEEIGYLRQELPSALKRVADGLGRIAE
                     VVRSIKDFARADISEMAAVDINQSIKSTLVVARSEYQAVAEIQTDLGELPAVVCHGGQ
                     INIVILNLLVNAAHAITDVKGSSERGLITVRTRAEGDNVVISVSDTGGGIREEIRHRI
                     FDPFFTTKEVGRGSGQGLTMARNVVVKVHGGTIDCTTEIGEGTTFEIRLPTRPTEMAA
                     PRGGQSE"
     gene            complement(3186..>4124)
                     /locus_tag="VFR82_11895"
     CDS             complement(3186..>4124)
                     /locus_tag="VFR82_11895"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU4502342.1"
                     /translation="TRRNLGIARRLAENGIAVLTYDKRGVGDSQGEYSGPLSTNEVTL
                     NALAADASAALEFLSAQSEVSDAKLGFAGISQAGWVIPLAAVASDKEAFTVVWSGMVG
                     RISEEDIFSLYTSDRDGADLPGFEEIVAQRVRPYAWPPELGEDVNSVDSLAQLDKPGL
                     WVFGGRDGSIPVPLSIARLEELQRDEHAFEYVLFSSAGHDTVETSFPVVADWIKRVGA
                     ASGQAVASLTAPLETFVGNYVMDDPPVSVGVSAESKELVITSQGDSLPAVRTGDASFY
                     MLVPGEGFYYFDFDPREDSLVMNAQGRAFTLRRTSR"
BASE COUNT          818 a         1277 c         1189 g          840 t
ORIGIN      
        1 gacctatcct gagccattct acggtcatgg aacgtggagc agcacctatc tgtcgaagct
       61 gaatatcgaa actaacaagg tgtcgtcggc cggcgcccga ttgccccgtg gcaattactg
      121 gaagttcgtc ggcgacgggc gcggcagcgc gattctcgct gccggcttag aagatggtat
      181 ggcgcccaaa gcctggattg cggcggcgcg agtatcaaac tcggcgccgt ggatccggct
      241 ctcgcgcctc gcgccccatg ccaccggcct gcgcttcgag cccgttgcgc tcgctgccgg
      301 caccaccgat gctttcatgc tcatggatca ccagggccgc agcgcactct ggcgcgtgga
      361 cctctccgat caacgcgacc cgatcctcga actgtggcac aaggagttca acgtccatga
      421 cgtgctgaga gacggtgacg gcgccttaat gggcgtccgc cttgcgacgt ccgccgccgg
      481 gcctctgtac cttgacggcc aggctcgcaa actcgatgca ctgctgcgcg caaaatggcc
      541 cgaccggcgg aacgaattcc tcgacgcaac cgccaatctc aaggaagtcg tggttcgcac
      601 ttggggcgac ggaagcgtac ctacctatca tgtgtttagt ggcggcactg agagtaccaa
      661 actagacttt gtcggatcgt cggcacccga aatggcgaaa ctatcgttgg tgcctagccg
      721 gtcgatcatg gtgtcgacag atactggccg ccacattttt gcgcagctta cgctcccggc
      781 aacgtccgac gaacacaagc ggcctctggt agtactggtg agcgactggg atgaggatgg
      841 actggtattc gatccccttg cccagctact ggcgagccgg ggctacgctg tggtgcgtac
      901 ccaagcggcg aatagcagcg ccgatgagag ttgggaactc gcccctctcg ttgactggga
      961 cggcaaagtc tatacggata tgctgactgt aatacgctcg ctgctcgccg acgacaggct
     1021 ggatgcggga cgtgcgtgtc tgatcggcac cggctacggc ggctacgtag ccatgctcgg
     1081 aaacatccgc ggcgacgtgc aacccgcctg tgcgatcgga atcaacacgg tcacggacct
     1141 cgcaagcgcg ctggcgaacg cgcgcaacag tgggctgcgc agggtcgtcg aaaggccggg
     1201 tcgactcgac ctcctcgcga agggggccga tccaatccgc aaacacgctg catccgcgca
     1261 cggccctaca ctcttggtaa acgatactct catcaaggaa gccaaggcgt tcgcggccgc
     1321 actttcgaag gaaaacaaga accaaaagct gatcatcacc gacctgatag gcgacgggtt
     1381 cgttgcacag acaaataggg aaattgtggc gttccttgat gagcacctgc cgatcgcgga
     1441 gcgttcagga gagtgaagtc ttgatgcaga tgatttgaaa gtcgcctcgg gaaattggcc
     1501 tactccgatt gtccgccgcg cggggcagcc atctctgtgg gacgcgtcgg caaccttatc
     1561 tcgaaggtcg tcccctcgcc gatttccgtg gtgcagtcga tcgttccgcc gtgcaccttg
     1621 accaccacat tgcgcgccat cgtcagacct tgaccgctgc cgcggccgac ttctttcgtt
     1681 gtaaagaagg ggtcgaagat acggtgccgg atttcctcgc gtatgccgcc accggtgtcc
     1741 gacactgaga taaccacgtt atccccttcc gcgcgcgtgc gcactgtaat caggccacgc
     1801 tcactgcttc cctttacatc ggtgatcgca tgagcggcat ttaccagaag atttaggatg
     1861 acgatattga tctgcccacc atgacacacg acggcgggta gctcgccaag atcggtctgg
     1921 atctcggcaa ccgcctggta ctcgctgcgc gccaccacga gggtgctctt tatcgattga
     1981 ttgatatcca ccgccgccat ttcactgatg tcggcacggg cgaagtcttt gatggagcgg
     2041 acaacttcgg caatgcgccc gagcccgtcg gcaacgcgtt tcaacgcaga gggcaattct
     2101 tggcgtagat atccgatctc ctcttccaca tcgcttggcc gttcggcgcc accagccggg
     2161 ttttcgagat attcgagcag ctcgtgaact ccttcgtcga tgaatcgaac actgtcactg
     2221 agatactgga tgggggtatt gatctcatga gccaaaccgg cagcgaggcg accgatggac
     2281 tcgtggcgat ccattgccat cagcttctga tccaccgcct tgcgctcggt gatgtcgtac
     2341 cccgtgccaa cgattcctgt gagtctgccg ttgtcatcct tcagtggaac tttggacgtg
     2401 aggatcaccc gattttctcc gcgcaccttg tcaaaagcga cttcctcgag atcaatcaag
     2461 ggttgtccag aggcaatgag cgcctgctct tcagcgaaaa acttctccgc catctcccga
     2521 ggaaagaagt cgaagtctgt cttgccaatc atctctgacg gaattgttcc caatcgcttc
     2581 gcgaccagcg cattgcacgc cgtaaaccgg ctgtgcgcat ctttcgcata gatgaatgcc
     2641 ggaatgagat cgatcatcgt acgcaggaca gtgcgctcgt gggccaggct ttccgcaagc
     2701 ttgcgcagcg cctctggctc ttcccccttc gaaggatgct gcctaggcag ctcgttccca
     2761 gatttagtat ccatgcttgg ctctcctggc cgaccgaaaa cattcatggc ttggaacacc
     2821 cgcgcgtccc gcatagcatt ctgcgtatcg tagtgccaca ttgagcgtaa gcgtcctgcc
     2881 aaggcaatgt tgacggggag taagatttcg tagcggatgt tggccgatgc ggtattgact
     2941 tcgtcgacga ggcggccgga ggattgggcg gtggcggcat cgagccgctt gaggggcgcg
     3001 tcaaggacct cgtcttcccg acgacgcaac atggtgagag ttttcatgcc gccgtgcgcg
     3061 acggtggtct acgggatggg tttggcggca cccactatga tttgcccgct gcccttgagc
     3121 attccactga tgttgggtaa tgcggaggca ttcacgcagc ggcctcattc gctccccttc
     3181 gggttctagc gggaggtacg tcgaagtgtg aacgctctgc cctgcgcatt catgaccagt
     3241 gaatcctccc gcgggtcgaa gtcaaaatag tagaagcctt cgccaggcac aagcatgtaa
     3301 aacgacgcgt cgcccgtgcg caccgccggc agcgaatctc cctgtgatgt gataacgagt
     3361 tccttgctct ctgcgctgac acccacgctg actgggggat catccatcac gtaattgccg
     3421 acaaacgttt ccagcggcgc cgtgagcgaa gcaacagctt gccccgacgc cgcgcctact
     3481 cgcttgatcc aatctgcgac aacaggaaag gacgtttcga cggtgtcatg tcccgccgac
     3541 gagaacagca cgtactcgaa cgcatgctcg tcgcgctgca gttcttcgag ccgcgcgatc
     3601 gaaagcggaa cgggaatact gccgtcgcgc ccgccgaaca cccagagccc cggcttatcg
     3661 agctgagcca ggctgtccac tgaattcaca tcctcgccaa gttccggtgg ccacgcgtac
     3721 ggcctcacgc gctgcgcaac gatctcctcg aagcccggca agtcggcgcc atcccgatcg
     3781 ctcgtgtaga ggctgaagat gtcttcctcc gagatccggc ccaccatccc gctccagacc
     3841 acggtgaagg cttccttatc acttgccacg gcggcgagcg gaataaccca gccggcctgg
     3901 ctgatgccgg caaatccgag cttcgcatca ctgacctcgc tctgtgcaga cagaaactcg
     3961 agcgccgccg acgcatccgc agcaagcgcg ttgagcgtga cctcattggt gctgagcgga
     4021 ccggaatact cgccttgcga atcgcccacg ccgcgcttgt catacgtcag cacggctatg
     4081 ccgttttcgg cgaggcggcg cgcgatcccc aggttgcgcc gagt
//
LOCUS       DASTQY010000363         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_791312, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000363 DASTQY010000000
VERSION     DASTQY010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791312"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /gene="tpiA"
                     /locus_tag="VFR82_11900"
     CDS             <1..734
                     /gene="tpiA"
                     /locus_tag="VFR82_11900"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU4502343.1"
                     /translation="TASEAAGFVAELSRAIPNNPTVEIVIAPPFTALASVRTALRPAS
                     NIALGAQNMHWEQQGAFTGEISGAMLRDLGCTYVLVGHSERRTLFGEQDGDAHKKVRS
                     ALINGLRPILCVGESLDQRTAGQTESLVTGQLAAALTDLTSNDLERVTIAYEPVWAIG
                     TGHAATTEQAAKVHRTIRAHIAAGWNQATAARMRILYGGSVTPQNAESLLSESEIDGA
                     LVGGACLKTEAFATIALAALNRRSG"
     gene            755..1096
                     /gene="secG"
                     /locus_tag="VFR82_11905"
     CDS             755..1096
                     /gene="secG"
                     /locus_tag="VFR82_11905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU4502344.1"
                     /translation="MIIIHIIACFLMIGAILLQSGKGAEIGAAFGGSSQTVFGSRGPA
                     NFLSKFTVVVAAVFMLTSMGLAILAKEKTFSSTVIDLKKKDTSQEVPATAPAQKPDTD
                     AHSSSDSPTAH"
     gene            complement(1168..2079)
                     /locus_tag="VFR82_11910"
     CDS             complement(1168..2079)
                     /locus_tag="VFR82_11910"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEU4502345.1"
                     /translation="MDTQGKIWMDGSFVEWADANVHVLTHSLHYGLAAFEGLRCYKGK
                     SGSAIFQLQAHVDRLFESAHISMMNIPYDKKQVAEAILDTVRVNRLEACYIRPLLYIG
                     YGAMGVYPGDNPIKLAIAAWKWGSYLGDDALANGMRACVSSFTRHHVNVSMTRGKISG
                     YYVNSILAKREAKADGYDEAILLDTEGYVSEGTGENIFIVRQGRIKTTPLTSILEGIT
                     RNAVIELAREQGIVVVEERFTRDEMYIADEVFVTGTAAELTPVREIDNRRIGTGKPGV
                     VTAALQKRFFAIVRGEDPSHDSWLTRV"
     gene            complement(2149..2520)
                     /locus_tag="VFR82_11915"
     CDS             complement(2149..2520)
                     /locus_tag="VFR82_11915"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4502346.1"
                     /translation="MKRAGTSTPCGGFVVRPYRRIPTWYGSYYLSGSVIGKGVVMNVS
                     CTGLRVLGDHSLKPGTDVSIRVTVDSGPPLEISRASVRWVKEYEFGLKIDQVTSQTAN
                     RLARLIHDSIGGRPSPVRENA"
     gene            2666..2980
                     /gene="rplU"
                     /locus_tag="VFR82_11920"
     CDS             2666..2980
                     /gene="rplU"
                     /locus_tag="VFR82_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU4502347.1"
                     /translation="MYAIVETGGKQYRVENGTVVQVESLSGDVGGTVELSAVRLVHGD
                     NGVVVGQPLVKGAKVTAEIVDQGRTRSIMVFKKQRRKNYRRTKGHRQSFTKLRITGIH
                     TA"
     gene            3028..3291
                     /gene="rpmA"
                     /locus_tag="VFR82_11925"
     CDS             3028..3291
                     /gene="rpmA"
                     /locus_tag="VFR82_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU4502348.1"
                     /translation="MATNKGGGSSRNGRDSNPQYLGVKAYGGETVKAGAIIVRQRGTK
                     FFPGFNVGLGKDHTLFARVSGIVKFEGGRARQKVSVYPVSATS"
BASE COUNT          770 a          996 c          969 g          704 t
ORIGIN      
        1 agacggcctc ggaggccgcc ggcttcgttg cagaattgtc ccgtgccatt cccaataatc
       61 cgacggttga aatcgtgatc gccccgccct tcaccgcatt ggcgtccgta cggaccgcgc
      121 tgcgcccagc atccaacata gcgcttggcg cccaaaacat gcactgggaa cagcagggcg
      181 cctttacagg cgaaatttcc ggagcgatgt tgcgcgacct ggggtgcaca tacgtgctcg
      241 tcggacactc cgagcgacga acgctcttcg gcgagcagga cggggacgct cacaagaaag
      301 ttcgctcggc actgatcaac ggccttcgtc cgattctttg tgtgggtgaa tccctcgacc
      361 aacggacagc cggccaaacg gaatcgctcg tgactgggca gctcgccgca gcactgacag
      421 atctgacatc caacgatctt gaacgagtca ccatcgccta tgaacccgtg tgggccatcg
      481 gcacgggaca tgccgccaca accgagcagg ccgcgaaagt ccaccggact attcgcgcgc
      541 acattgcagc cggctggaac caggcgacgg cggcacgcat gaggattttg tatggtggaa
      601 gcgtgacacc gcagaatgcg gaatctctgc tgagcgaatc ggagatcgat ggcgcgctcg
      661 tcggcggggc ttgtctcaaa acagaggcat ttgctacaat tgcgctcgct gctttgaacc
      721 gacggagtgg gtgacagagt ctatgcatac tttggtgatc atcattcata tcattgcatg
      781 cttccttatg atcggagcga ttctcctcca gtcgggaaaa ggagcggaaa tcggcgcggc
      841 cttcggcgga tcgagccaga cggtattcgg tagccgcggc ccagcgaatt ttctcagtaa
      901 gttcacggtc gtggtcgcag cggtcttcat gttgacctcc atgggacttg cgatacttgc
      961 gaaggaaaag acgttttcgt ccaccgtcat cgatcttaag aagaaagaca cttctcaaga
     1021 agtgcccgcc actgcacccg cccaaaaacc ggacacggac gcacactcct cgagcgattc
     1081 ccctaccgcc cactgatcga cacgaagccc ggacaaattc ggtgtggcta gacttgggtg
     1141 ggattgaaga agtttctcag cgtctcgcta gactcgggtg agccaggaat catgcgaggg
     1201 atcttctccc cgcacgatcg cgaaaaaccg tttttgcaat gcggccgtca cgacccccgg
     1261 cttcccggtc ccgatccggc gattgtcgat ttctctgacc ggagtcagtt cggcagccgt
     1321 ccccgtcaca aacacttcgt cggcgatgta catttcatcg cgcgtgaacc gctcttcgac
     1381 cacgacgatg ccctgctccc gtgctagttc gatgacggca tttcgcgtaa tgccttcgag
     1441 gatggatgtc agcggcgtcg tcttgatgcg gccctgccgc acgatgaaga tattctcgcc
     1501 ggttccctca gacacgtacc cttccgtatc cagaagaatg gcctcgtcat agccgtcagc
     1561 cttggcttcg cgtttggcta agatcgagtt gacgtagtac ccggaaatct ttcctctcgt
     1621 catcgacacg ttgacgtggt gtctcgtaaa cgaggatacg caggcccgca tgccgttagc
     1681 caaggcgtca tcgccgagat acgaacccca cttccacgcg gcgatcgcca gtttgatcgg
     1741 attatcgccc ggataaacac ccattgcgcc atacccaatg taaaggaggg gacggatgta
     1801 acaggcctcc aggcgattga ctcggaccgt gtcgaggatg gcctcggcca cctgtttctt
     1861 gtcgtaggga atattcatca tgctgatatg ggccgactca aacaatcgat cgacatgtgc
     1921 ctgcagctgg aaaatggcag aaccggactt tcccttgtag caacggaggc cctcgaaggc
     1981 ggccagtcca taatgcaaag agtgggtcaa gacatgcacg tttgcatccg cccattcgac
     2041 gaacgaaccg tccatccaga tcttgccttg cgtgtccata ctggagcgga ctatagcagc
     2101 ggttttgaag gagcgtcaag caaacgagga agttgaaagc gcggtacgct atgcgttttc
     2161 ccgcacaggg gaagggcgac ctccgatgga atcgtggatg agtctggcta gtcgatttgc
     2221 agtctgagac gtgacctgat cgatcttcag cccgaattca tactccttga cccagcggac
     2281 cgaggctcga gaaatttcca gaggaggacc actgtccaca gtcacgcgga tagacacgtc
     2341 ggttcccggc ttgagagagt gatctccgag cacacgcagt ccggtgcaac tcacgttcat
     2401 gaccacgcct ttgccgatca cgcttccgct caggtagtag gaaccatacc aggtggggat
     2461 gcgccgatac ggacggacca caaaccctcc gcacggcgtc gatgttccgg ctctcttcat
     2521 agcttcactt tacgccgccc ctactggcca aaaacagcct acgaacaggg ggggacatgc
     2581 gactcgccgc caacgcgtcc tcttccttga cactcttgaa atcactgtga tacataacga
     2641 cgttctgtta gggaggcagg gactcatgta tgcaatagta gaaacaggcg gcaagcagta
     2701 tcgagtcgag aatggaactg tcgtgcaagt ggaaagtctg tccggcgatg tgggcgggac
     2761 cgtggaactg agcgctgtgc gactagtcca cggcgacaat ggagtggtcg tcgggcagcc
     2821 gttggtcaag ggcgccaagg tcaccgctga aatcgttgac cagggccgga cgcgttcgat
     2881 catggtgttt aagaaacaac gccgcaagaa ttatcgccgc accaagggac atcgtcaaag
     2941 tttcaccaaa ctacgcatca caggcatcca tacggcatag ttgtcggctc ataaagagcg
     3001 atatcgcacc cgaagcaagg atctgtcatg gcaacgaata aaggcggcgg ttcgtcaaga
     3061 aacgggcggg acagcaaccc tcaatacctc ggtgtgaaag cctatggcgg tgagaccgtg
     3121 aaagcgggcg ccatcatcgt gcgtcaacgg gggacgaagt tcttccctgg ttttaatgtc
     3181 ggcctcggca aagaccacac cctcttcgcc cgcgtgtcgg gaatcgtaaa attcgagggc
     3241 ggacgcgcgc gccagaaggt cagcgtctac cccgtgtccg cgacctctta acatcgcaca
     3301 cttctcctga gtcatcacgc agtccgtacg ggctgacggc gagtttgcgt gcagcggttc
     3361 tcgcgcagtc cgcatcgtca cgatttcttt ttgagtgtcc atgagtaggg catcatgttc
     3421 gtagacgagg tcaccatca
//
LOCUS       DASTQY010000364         4059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_416433, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000364 DASTQY010000000
VERSION     DASTQY010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416433"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFR82_11930"
     CDS             <1..203
                     /locus_tag="VFR82_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU4502349.1"
                     /translation="PITERILTGSRMDRSLYVMLTALIQSGEPKLLRAAEMSLKSLVK
                     QSKQSSVPWQNRPSGHFEPPSD"
     gene            complement(333..1526)
                     /locus_tag="VFR82_11935"
     CDS             complement(333..1526)
                     /locus_tag="VFR82_11935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU4502350.1"
                     /translation="MTGDDERSTGTVTLARAKEIVGSGHLWIYAGFVHAVAGQPVAGD
                     LVDVLMPNGRFYARGFYNPHSKIRIRILTFMDERIGPAFWHERIAQAVRLRKRIVSGA
                     TAYRVIYGEADRLPGLIVDRYGDVLVMQTLSNGMDRRKEMLADLLCKETGMSKVYLRN
                     DAKSRLLEGLRPVTGFLCGNGATTIEVQEGPARFLVDVAQGQKTGWYCDQRENRLAAA
                     RFAMGKAVLEVFCHTGAFGIQAALAGAQLVEGLDISEEALVVARNHARLNGVQDRCSY
                     RSADAFDEMRRLVQVRGQYDVVILDPPAFARSKQAVPRALAGYKDINLLGIRLTRSEG
                     ILVTSSCSHHVSEMDLWKVLAQAAQDANRPVRLLEQRGQASDHPVLFAMRETRYLKCF
                     ILQVL"
     gene            1566..3323
                     /locus_tag="VFR82_11940"
     CDS             1566..3323
                     /locus_tag="VFR82_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU4502351.1"
                     /translation="MASPARICLTGCLLYGLLERETLLAQHIELQTESMGIEATIGSA
                     VIDRLHQLGVRHIFGIPGDYVLSLYDLIERSPIRHIGTTREDCAGFAADAYARINGIG
                     AACVTYCVGGLNCVNAIACAYAERSPVVLLTGSPGLSERIRTPYMHHMVRDFSTQREV
                     FERMTVASISLDDPTTAEREMDRAFAALLRYRRPIYIEIPRDLVHTPLPHPLRPLCLN
                     EDPSDPAALEEAIGEVRAMLASAKRPAVLAGAEIGRFGLQDDLSRLVERLKIPIASTL
                     LGKSVIREDHPLYVGVYGGLIARDEVQQFINDSDCLLILGSILSDLEDLNADSPLRSE
                     GRTIHATADHVSIRHHRYDTIRFQDFVQALGTHGLSTQSSRPLPPPLSITGPPPAPDS
                     QVTLRGLFGHLDQKLDQTTLVIADVGESLFAAADLRVHRRFEFLSPAYYTSMGFAVPA
                     SIGASFADPTLRPIVLVGDGAFQMTGSELSTAVRYRQSPIVIVLNNRGYSTEREILEG
                     PFNDIHDWRYERICDLIGGGMGFVVRTQQEFERTLEKALADSGQLYVLNVLLDPADRS
                     PGMIRLAHRLAKRLSTDRP"
     gene            complement(3475..3681)
                     /locus_tag="VFR82_11945"
     CDS             complement(3475..3681)
                     /locus_tag="VFR82_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502352.1"
                     /translation="MHYWVKIVFVDNQELVVKDAVRHTISDDMEVLEVDSAKEVIIVP
                     MKQIKYLACDATVFASKGAHQPKG"
     gene            complement(3697..>4059)
                     /locus_tag="VFR82_11950"
     CDS             complement(3697..>4059)
                     /locus_tag="VFR82_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HEU4502353.1"
                     /translation="FESIGQIVKVEEGALDAVTGLSGGGPAYVYVMIEALADGGVKAG
                     LPRETAQLLAAQTVSGAARMVLESREHPGMLKDRVASPGGTTISGLHELERGGLRATL
                     ISAVEAATRRSQELGRAV"
BASE COUNT          844 a         1233 c         1058 g          924 t
ORIGIN      
        1 atcccatcac cgaacgaatc ctgaccggtt cgagaatgga tcggtcgctc tatgtcatgc
       61 tgacagcact gattcagagc ggggagccca aactgcttcg ggctgctgag atgagtttga
      121 aaagtctagt gaaacagtcc aaacaatcct cggtcccttg gcagaatcgt ccgtcaggtc
      181 actttgagcc accgagtgat tagtcgctac acaatgcggc ttgatccttg acctacctcg
      241 ctccccaaaa gttcaggctc catatctcat agcccctttt tcatattcgc atggcttcct
      301 cgctgctatc gtttgggaaa tagcgcgtga tttcaaagga cttgaaggat gaaacacttg
      361 aggtaacggg tttctcgcat ggcaaagagc acgggatgat cggacgcttg acctcgttgt
      421 tcaagcagac gcaccggacg attggcgtcc tgcgccgcct gtgcgaggac cttccacaaa
      481 tccatctcgg aaacatggtg ggagcaagaa ctcgtgacaa ggatgccttc ggatctggtc
      541 agtcgtattc ccaacagatt gatatcctta tagccggcga gggcccgggg gaccgcctgt
      601 ttgctgcggg caaaggcggg agggtcgaga atcacgacat cgtattgccc acgcacctgc
      661 accaggcgac gcatctcgtc aaaggcatcg gccgatcggt atgagcaccg gtcttgcacg
      721 ccgttcaatc tggcatgatt ccgtgccacg acaagcgctt cttcactgat gtccaaccct
      781 tcaactaact gggcccccgc gagggccgcc tgtatcccga aggcgcccgt gtggcaaaag
      841 acttccagca ccgccttccc cattgcaaac ctggcggccg caagccgatt ttcgcgttgg
      901 tcgcagtacc agccggtctt ctgaccttgc gcaacgtcga cgagaaaacg cgccggtcct
      961 tcctgaactt ctatcgtcgt ggcaccattc ccgcataaaa accccgtgac gggcctcaag
     1021 ccctctaaca gccggctctt ggcgtcattc cgaagataca ccttgctcat tccggtttcc
     1081 ttacacaaaa gatccgccag catctccttc cgacgatcca ttccgttcga cagcgtttgc
     1141 atgactaaca catcaccgta ccgatcaacg atcaaacccg gcaggcgatc agcctcgccg
     1201 tagatcaccc gataggccgt cgctccggaa acgatccgct ttcgaagccg cacggcttgg
     1261 gcgatgcgtt cgtgccagaa tgcaggtccg atccgttcat ccataaaggt caagattcga
     1321 atacggattt ttgagtgggg attgtagaat ccgcgggcgt agaatcgacc gttcggcatc
     1381 aggacatcga ccaagtcgcc ggcaaccggc tgtccggcga ccgcatgcac gaaccctgca
     1441 tagatccaga ggtggccaga gccgactatt tccttggccc gggccaaggt gactgttccg
     1501 gtcgaacgtt catcgtcccc tgtcatggaa tagttatcgg atgaggcgag tccaacgtca
     1561 aggccttggc ctcccctgct cgtatctgct tgacgggttg cctcctctat ggtttgctgg
     1621 aacgagagac tcttctcgca cagcatatcg aactgcagac ggaatcaatg gggatagaag
     1681 cgacaatagg ctcagccgtc atcgatcggc tgcatcagct cggtgtccgc catatatttg
     1741 gcattcccgg tgactacgtc ctcagcctgt acgaccttat cgagcgctca ccgatccgcc
     1801 acatcggcac gacccgagaa gactgcgcgg ggtttgctgc ggacgcctac gctcgcatca
     1861 acggcatcgg tgcggcctgt gtcacctatt gcgtcggcgg cctgaattgc gtcaatgcca
     1921 tcgcttgcgc ctatgctgaa cgttcacctg tcgtgctctt gaccggatca cccggcctgt
     1981 cggaacggat tcgtacgccg tacatgcacc atatggtgcg cgatttttct acgcaacgag
     2041 aagtctttga gcgaatgacg gttgcctcca tttcgctgga cgatcccacg acggcagagc
     2101 gtgaaatgga ccgggccttt gcggcgctcc tgcgctatcg ccggccaatc tatatcgaaa
     2161 ttccacgcga tctcgtccat acccctttgc cgcatccact gcggccgctc tgtttaaacg
     2221 aggatccaag cgatccggcc gcgctggaag aagccattgg agaggtccgt gccatgctgg
     2281 catcggcaaa acgtccagcc gtgcttgccg gcgccgaaat cgggcgcttt ggtcttcagg
     2341 atgatttgtc gcgtctcgtt gagcgtctga aaattccgat cgcctcgacc cttttgggca
     2401 aatcggtcat tcgagaggac catccattgt atgtgggcgt ctacggagga ttgatcgcac
     2461 gtgatgaagt tcaacagttc atcaacgatt ctgattgcct cttgattctc ggatcgatct
     2521 tgtccgatct cgaggatttg aatgccgatt cgccgttgcg gtccgagggc cgcacgatcc
     2581 atgcaacagc cgatcacgtc agcattcggc atcaccgcta cgatacgatt cgattccagg
     2641 acttcgtcca ggcccttggg acgcatggtc tatcgaccca gtcttctcga ccccttccac
     2701 cacccctctc gatcactggg ccgccccctg caccggactc acaagtgacc ttgagagggc
     2761 tgttcgggca tttggatcag aaattggacc aaaccacgct tgtcattgcc gacgtcgggg
     2821 aatcactgtt tgcggcagct gacctgcgtg tgcatcgacg gtttgaattt ctatcgccgg
     2881 cctactacac ctcaatggga ttcgcggttc cggcgtcgat cggagcgtcg tttgccgatc
     2941 ctacgctccg tccgattgtc ctggttggtg acggcgcctt tcagatgaca ggatccgagc
     3001 tttcgaccgc cgttcggtat cggcaatcac ccattgtcat tgttttgaac aatcgagggt
     3061 attccaccga acgtgaaatt ctggaaggtc ccttcaacga cattcatgac tggcggtatg
     3121 aacgaatctg cgatctcatc ggcggcggca tgggcttcgt cgtgcgaacg cagcaagaat
     3181 tcgaacggac tctggaaaag gccctggccg attccggtca gttgtacgtt ctaaacgtcc
     3241 tgctcgatcc ggccgaccgc tcgcccggca tgattcgcct ggcccatcgc ctggcaaaac
     3301 ggctctctac cgatcgcccg taacttacat cagcatcact gcacagtcct tttcatgacc
     3361 gcctttgggc cccgatttgg gcaatggagg ttgggcacac cgaggactag acggaagctg
     3421 aaattatgta tcaaagaaag gaaataacgc agggctctca gaaggcgggt cctgctagcc
     3481 tttcggttgg tgtgcacctt tcgacgcgaa gaccgtcgcg tcacaggcaa ggtacttgat
     3541 ctgcttcatg ggtacgataa tcacttcctt cgccgaatcc acttccaaca cttccatgtc
     3601 gtcactgatc gtatgcctga cggcatcctt gacaaccagt tcttggttat ccacaaatac
     3661 aatcttcacc caatagtgca cgatagggta ctccttctag acggctctac ctaattcttg
     3721 tgaccgtcgt gttgcggctt ccactgctga gattagcgtc gcccgcagac cgccccgctc
     3781 gagttcatga agtccagaaa tagtcgtacc tccgggcgaa gcaacacggt ccttcagcat
     3841 cccgggatgc tcccgtgact cgagtaccat ccgcgccgcg ccactgacgg tttgcgctgc
     3901 cagaagctgg gctgtctcac ggggcaagcc cgccttgacg cctccatccg ccaatgcttc
     3961 gatcatgaca tagacatagg cgggtcctcc cccactcagg ccagtgacgg catcaagcgc
     4021 cccttcctcg actttcacga tctgtccgat ggattcgaa
//
LOCUS       DASTQY010000365         4503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_74166, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000365 DASTQY010000000
VERSION     DASTQY010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74166"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(814..1653)
                     /locus_tag="VFR82_11955"
     CDS             complement(814..1653)
                     /locus_tag="VFR82_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502354.1"
                     /translation="MSTKLELIREEKLFRLLPGRTKHSRLEASGVALVDANTALVVFD
                     NINQVARIDLSLNRQASNALLPAPSLGLGFEDVGIDQRHSRVFCLVESVEDCDGLLHG
                     FVAEYDHGGRFIRCSRLPVRLEKANKGFEGIEHEWRAGREYLYALYEAAHKPGKGRIE
                     VFVRAHDGGWKISHQIHLPKKAEFEDYAAMSYRFGKLAVVSQASARVWVAHIDEKARA
                     VVPGSQAIYRFPNKRYENVEGIAWLATDTLVAVSDRKKKRQPDRCSDKDQSIHLFRIP
                     AGD"
     gene            1873..2583
                     /locus_tag="VFR82_11960"
     CDS             1873..2583
                     /locus_tag="VFR82_11960"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4502355.1"
                     /translation="MVKSSLVSEIIRLAGVTILGSALLLTQTCPAYADETVDKAIRKG
                     KWDIMLAPQYTLAKNLGFDGGTTAKIDDTVGFNFQFGYNFNEHWNLGGLFSWSQPDYQ
                     AVLQPVQGNPAGPRSQHGTIQTNTFALVTTYHFLAGPVTPFVDANLGGTHVNTDVADG
                     PPVIGCYWDPWFGNVCGVAQPTKSGTYLSYGVGGGLRWDVNHWLFFRGAVRQQWIDLP
                     NTGVPSFTIFKLDVGFKF"
     gene            complement(2725..4011)
                     /locus_tag="VFR82_11965"
     CDS             complement(2725..4011)
                     /locus_tag="VFR82_11965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEU4502356.1"
                     /translation="MKTVAHDRRRLRSASTGEPIRSFLRHYKAFALAIGVALYYFRFT
                     LHPDGMTLYPMAADCMLNGHPMGSCSPGFTYPPLFGFTMIPFVFLPMWARNVLWYAVL
                     IAATWGSFRLAELLVCQLPGIQLDKTELTRFRVVSLMLVLNFVLANLENQAYDVLVLL
                     FLLIGLVGLTRESSVWSTIGFGVAAALKATPLLFFPYIFFKKGWKLFTLCVVCYLTLS
                     LLPDLVLKVENGLTTYYGNWIRQVALQPFLAGDSGGMPRFWEGENDLNQSLRALVYRI
                     TLGLDIQQHFVLMLYGTYSLCLIYMLWVFSRSFRLHNHVVFDGSLIIIMMLLFSPMSS
                     KSHFVALMLPFMVLTGYMMSDSQLRSGLIFASVLSCALNNLTSKDLVGSQLSGVAGYA
                     GSVTLGTITLALAIGYLVNLKQKSFLSFDHQTPRAF"
     gene            complement(4171..>4503)
                     /locus_tag="VFR82_11970"
     CDS             complement(4171..>4503)
                     /locus_tag="VFR82_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502357.1"
                     /translation="SRAANHLSRLALGLTVRDVTAGFRCYRRELLSTLDQIEMRSNGY
                     SFQLEVTYITHALGYRIREVPIIFHDRIHAKSKLGTGELLAAAQTLFRLGMHHASGEG
                     LSQPGRKV"
BASE COUNT         1068 a         1184 c         1241 g         1010 t
ORIGIN      
        1 acatgtgtgc atccggacga tggatcaacc ttggcgcact caacgatttt tcccatggta
       61 gctcctcctt gttgagccag aacctgccaa ccttcgataa ctgctgtgaa aacctcgtta
      121 aatggatgac caaacgaagg caaattctga ggacattgga agagagagtc aatggcgcta
      181 catgcagtag tcactagctt tgaaatcaag caatgaccga tcagaaatgc cggaagagat
      241 ggacaagcct ccccccacgc cgaatggtac gaaatgggtg tatcttctag aaagatagaa
      301 tgttcccctt ttcttgtttc aagggggctt gctaggtacg cgttgtccgg tgaggacagt
      361 agactaattc tacgaaatgc gcgacagaac aatctcccct gttcgctggg tacaaagtaa
      421 aagatgtaga taactccgca tggcgatgga ggttagcatg attcctatac tctgcactct
      481 gcccattgtc cttatggccg gcgtcatggt cgctggctgt gctgatgacc ctaatcccgg
      541 tcgtcaacga cccgcgtata gttacgagaa cgtcgacatg ggatcaccac aacctggtca
      601 ataccgtttc gacgaacgcc cgccagcttc aaacagtgat ccttatagcc gtagccagcc
      661 tgggcgctat tagcggaacg cgatcgagat ccatcgcggt gtgaggtgcg atgagctgtg
      721 atgggtagga ggtgcgggga ggcggggagg cgaggaacga accggcccgc cacgcgttta
      781 ggcggtggtt tgacttgcca acaattgttt tgttcaatca cccgctggga tccgaaaaag
      841 atggatgctt tgatccttgt ctgagcaacg atcgggctgt cgtttcttct ttcggtcgga
      901 gactgcaacc agggtgtccg tagcgagcca ggcaattcct tcgacgtttt cgtagcgctt
      961 gttagggaag cggtagatgg cctgcgaccc aggcactacc gcacgggcct tttcatcgat
     1021 gtgagcgacc cacacacggg cagaagcctg cgaaacgacg gcaagtttcc cgaagcgata
     1081 cgacatcgcc gcatagtctt cgaactcggc ctttttcggc aggtggatct gatgcgagat
     1141 cttccatcct ccgtcgtggg cgcggacgaa cacctcgatg cggcccttgc ccggcttgtg
     1201 agctgcctcg tagagggcgt acaggtattc ccggccggcc cgccactcgt gctcgatacc
     1261 ttcgaaccct ttgttggcct tttccaggcg aacaggaagt cgagagcagc ggatgaagcg
     1321 gccgccgtga tcgtactcgg ccacaaatcc atggagcagt ccgtcgcagt cctccaccga
     1381 ctcgacgaga cagaataccc ggctgtgccg ttggtcgatg ccgacatcct cgaatcccaa
     1441 gcccaggctt ggtgcgggta agagcgcgtt acttgcctgg cggttgagtg aaaggtcgat
     1501 tcgtgccact tgattgatgt tgtcaaacac cacaagtgca gtgttggcgt cgaccagcgc
     1561 gaccccgctt gcctccagcc ggctgtgctt cgtcctgccc ggcagcaggc ggaagagctt
     1621 ttcctcccga ataagttcga gctttgtgct cacgagcgag tgaatggcga gtgctgcatt
     1681 ctcaaggaac ttccaaggag gccattctaa ttgatatcgg cggtcaggtc tattgccgca
     1741 cgaatagtct cccgcatcat cagcacctgt caatattgtc agtttccttc ctgcttggca
     1801 ttcgtataat tggaacactc gctgtttcca agcgttcgtg gatccgtctt aacgctgctc
     1861 gacgaggttg atatggtcaa gtcttcgctg gttagtgaaa tcatccgact cgccggggtc
     1921 acaattctcg gttctgcgct gttacttacc cagacatgtc ccgcttacgc ggatgagacg
     1981 gttgacaagg ccattcgcaa ggggaagtgg gacatcatgc tcgctccgca gtacacgtta
     2041 gcaaaaaatc tcggattcga tggcggcact accgcaaaga tcgacgatac ggtgggattc
     2101 aactttcagt ttggttataa ctttaatgaa cattggaacc tgggtgggct gttttcatgg
     2161 agtcaaccgg attaccaggc tgtcctgcaa ccggtccagg gcaaccccgc cgggccacgc
     2221 tcgcaacacg gaacgattca aacgaatacg ttcgccttgg taacgaccta tcattttctt
     2281 gccggccctg taactccgtt cgtcgatgcg aatcttggtg gcacacatgt gaatacggac
     2341 gttgctgatg gccctcccgt catcgggtgt tactgggatc catggttcgg gaacgtctgc
     2401 ggggtggccc aaccgaccaa gtcagggacg tacctgagtt atggagtggg aggagggctg
     2461 cgatgggatg tgaaccactg gctgttcttc cggggggccg tgcggcagca atggatcgat
     2521 ctacctaaca ccggggtgcc gagctttacc atcttcaagc tcgatgtcgg ctttaagttc
     2581 tgatgtgacc gctgtccacc acgcgccgcg aaaatggatt gcgcctttgt atatctgaat
     2641 cattttgttc ttgaggtgtg atgaccggtg atgggtagga ggggcgagga ggtgagatcg
     2701 tgccggcctg ccacgatcac aaggtcagaa tgccctcggt gtttggtggt cgaatgaaag
     2761 gaatgacttc tgttttagat tcaccaggta tccaatggcg agggccagcg taattgtccc
     2821 aagtgtcacg cttcccgcat acccggctac acctgagagc tgacttccga ccagatcttt
     2881 tgaagtgaga ttatttaggg cgcaactcag cactgaagcg aaaatcagtc cactgcgcag
     2941 ctgcgagtcg gacatcatgt acccggttag caccatgaat ggcaacatga gggcaacaaa
     3001 atgagatttg ctgctcattg gcgaaaaaag cagcatcata ataataatca gtgatccgtc
     3061 gaacacgaca tgattgtgaa gcctgaagga gcgcgagaac acccacagca tgtagatcaa
     3121 gcacagactg taggtgccat ataacatgag gacgaaatgt tgttgaatgt ccagtccaag
     3181 cgtaatgcgg tacaccaatg ctcgtaagga ctgatttaaa tcgttctctc cttcccaaaa
     3241 tcgaggcatt cccccggagt ctcctgcgag aaagggctgc agagctacct gtcgaatcca
     3301 gttgccataa taggttgtca gaccgttctc caccttcaaa acaagatcgg gcaatagcga
     3361 gagcgtgaga tagcacacca cacacagagt gaagagtttc cagcctttct tgaagaagat
     3421 ataagggaaa aacaacagag gcgtggcttt gagggcagct gcaacaccga agccgatggt
     3481 tgaccacact gaactttctc tcgtgagtcc aaccaggcca ataaggagga acagcaagac
     3541 caacacgtcg taggcctgat tctccaagtt cgccaggaca aaattcaaca caagcatgag
     3601 agatacaacc cggaagcgag tgagctccgt cttgtcaagt tggatgcctg gcaactggca
     3661 aacaagcaat tcagccagtc gaaaactgcc ccaggtagcg gcgattaata ccgcatacca
     3721 caggacgttc cttgcccaca taggcaagaa cacgaatgga atcatcgtaa agccaaacag
     3781 ggggggatag gtgaaacccg gactgcagct tcccatcggg tgaccgttga gcatgcagtc
     3841 cgctgccatt ggataaaggg tcatcccgtc cggatgaaga gtgaacctga aataatacag
     3901 agctactccg atcgccaacg cgaacgcctt gtagtggcga aggaaagacc gaatgggttc
     3961 accagtggag gcactcctca aacgacgtcg gtcatgagcg actgtcttca tggtgggcct
     4021 ccttacctat gaagtctgga cgctccagtg ggataatccg gcaggcatga ccgtgtgtgc
     4081 gcatgttcgc tctgggccca cagatgccct gcctgatagc tggattattt gtactgaccg
     4141 ccagcgctga cctttgaatc tggcgcctta tcaaactttc ctgcccggtt gggacaggcc
     4201 ctcaccactc gcgtgatgca tgccgagtct aaaaagggtc tgagcggcgg ccagtagctc
     4261 tcctgttcct aacttgctct tggcgtggat gcgatcgtga aaaatgatcg ggacttcccg
     4321 aatgcggtat ccgagagcgt gggtgatgta cgtgacctcg agctgaaatg aatagccatt
     4381 cgagcgcatc tctatttgat ccaatgtcga caacaattct ctccgatagc accgaaagcc
     4441 ggccgtcacg tcccgcacgg tgagccccag tgccaatcgg gagaggtgat tggccgcccg
     4501 act
//
LOCUS       DASTQY010000366         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1485194, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000366 DASTQY010000000
VERSION     DASTQY010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485194"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFR82_11975"
     CDS             complement(<1..576)
                     /locus_tag="VFR82_11975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4502358.1"
                     /translation="MSKARILLADDHMLLLDGLRHLLERHYDLVGVVQDGHSVVSAAQ
                     RLQPDLVLMDVAMPILNGLQAGQRLRERQPNVKLLYVSMYGDTPYVEEALRIGASGYV
                     LKRSGWEELSRAIETVLGGKQYVSPELFSALQRPEVQPPTTREADMRSLTDRQRQILR
                     LVAAGYTAKEIGRMLHITSKTVEFHKGRMMRQ"
     gene            complement(548..>2174)
                     /locus_tag="VFR82_11980"
     CDS             complement(548..>2174)
                     /locus_tag="VFR82_11980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU4502359.1"
                     /translation="LEVARSIARCIIRLPFHFLIVRETSYNQAIAFCGHPISLTAMNS
                     SGVKETRPQRWLIAAIACMTIAVFVLDSLFPLGFVVPALYLIPITLTTRVRYPLAPFL
                     VAASSTGLTFAGIWTNWTSPVVSLQLGLFNRLLVVLACWMTATIIWNTTQKYAREELE
                     ALVAARTAALRQSETTLQSFFNSTDLMMGVVEVIEDRPVMVITNRAASNAGDCTDHGS
                     ALLSPALRWASDSPTFWMPHLEAARRTGKTVHVKYQIPSTPATALSERIMTSALSFIG
                     YSAAGNGLYAWIGQDITATMHIEQKMEANRRSLEISQAQLRQLTAQLLTAQDDERRRI
                     ARELHDEVNQRVISLAFEIDDQLQQMPELPAAARTALQLVKNEVVELSDHLRELAHRL
                     HPSVLEDLGIASALRGCTQEFEQREHIPVHLALEEFQRPLDRHLAECLFRVTQEALRN
                     VAKHAGATTVSLTLSYHEDRVMLRIEDNGRGLAPQDRNAVQRGLGLVSMGERVRLLEG
                     TLTLTGDPGRGTRLSVSIPLTGISHVQSTHLTR"
BASE COUNT          457 a          603 c          656 g          458 t
ORIGIN      
        1 ttgccgcatc attcgtccct tgtggaactc tacggttttc gacgtgatgt gcaacatacg
       61 gccgatttct ttggccgtat agccggctgc gaccagtctc aaaatttggc gctgacgatc
      121 cgtaagcgac cgcatgtctg cctcgcgtgt ggtagggggc tgcacttctg ggcgctgtaa
      181 ggcggagaac aactccggag agacgtattg cttgccgccg aggacggttt caatggcgcg
      241 cgagagttct tcccaaccgg accgcttgag cacgtacccc gaggcgccaa tccgtaaggc
      301 ctcctccaca tacggtgtat cgccatacat ggagacgtag agcagtttta cgttcggctg
      361 ccgttctcgc agccgttgtc cggcttgcaa accattgagg atcggcatgg cgacatccat
      421 gagcacgaga tccggctgta gccgctgcgc ggcgctgacg actgagtgtc catcctgtac
      481 gacacccacg agatcgtaat gccgttccag aagatgccgc agcccatcga ggagcagcat
      541 gtgatcgtca gcgagtaaga tgcgtgcttt ggacatgcga aattcctgtg agcgggattg
      601 acacggacaa gcgggttccc ctgccggggt cgccagtgag ggtcagcgtt ccctccagaa
      661 ggcgcacgcg ttcccccata ctgaccagtc ccaacccccg ctggactgcg ttccggtcct
      721 gcggggcaag cccgcggccg ttgtcctcga tccgcaacat gacccggtct tcgtgatagg
      781 agagcgtaag tgagaccgtt gtcgcaccgg cgtgtttcgc gacatttcgc aacgcttctt
      841 gagtgacacg gaagaggcat tctgctaaat gccggtccaa tggacgctgg aattcctcca
      901 aagccagatg gactggaatg tgttcccgtt gctcgaactc ctgcgtgcac ccccgaagag
      961 cgctggcgat gccgaggtcc tcgaggaccg atggatgaag ccgatgggca agctcgcgca
     1021 gatgatccga taactcgacg acctcgtttt tgaccaactg cagtgccgtg cgggcggcgg
     1081 caggcagttc aggcatctgt tgcaattgat cgtcgatttc aaatgccaaa ctgatcacgc
     1141 gttggttgac ttcatcgtgc aactcgcgcg caatccgtcg tcgttcatca tcctgtgctg
     1201 taagcaattg tgccgtaagc tggcgtagct gggcttggct gatctccaag gaacggcggt
     1261 ttgcctccat cttctgctca atgtgcatgg ttgcggtgat atcttgcccg atccaggcat
     1321 acaggccgtt gccggcggcg ctatatccga taaaagacag cgccgacgtc ataatgcgtt
     1381 ccgagagcgc cgtggcaggc gtggacggaa tttggtattt cacgtgcacg gttttgccgg
     1441 ttcgacgggc cgcttcgaga tgcggcatcc agaacgtcgg cgagtcagac gcccatcgca
     1501 aggctgggga aaggagcgct gatccgtggt cagtgcagtc gcctgcgttc gaagctgcgc
     1561 gatttgtgat caccatgact gggcggtcct cgatcacttc gaccaccccc atcatcaaat
     1621 ccgtggagtt gaaaaaactt tggagtgtgg tctcgctttg gcgaagcgcc gctgtccggg
     1681 ctgcgacgag ggcttccaat tcctctctcg catatttttg cgtggtattc cagatgatgg
     1741 tggccgtcat ccaacaggcc agaacgacca ataatcggtt gaacagaccg agctgaaggg
     1801 agacgacagg cgacgtccaa tttgtccaga tgccggcgaa cgtcaggcca gttgaagagg
     1861 cggccaccag gaacggcgcc agtggatagc ggactcgcgt ggtgagcgtg atcgggatca
     1921 ggtaaagggc tggaacgaca aaccccagcg gaaaaagact gtcgaggacg aacacggcga
     1981 tggtcatgca ggcgatcgcg gcaatcaacc agcgctgcgg ccgtgtctct ttgacgcctg
     2041 acgagttcat agctgtcagg gagatgggat gtccacaaaa cgcaatggcc tgattatagg
     2101 aggtttctcg aacaatgaga aagtgaaaag gcagccggat gatgcaccta gcaatactcc
     2161 tagcaacttc gagg
//
LOCUS       DASTQY010000367         7493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_883534, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000367 DASTQY010000000
VERSION     DASTQY010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883534"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VFR82_11985"
     CDS             complement(<1..891)
                     /locus_tag="VFR82_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEU4502360.1"
                     /translation="MSAQTRDIIQIDYWPIDIPLTDPFVVATGSRSIAENVYVRMTLS
                     NGTQGYGEAAPFPEVGGESRASCLAAIGLVGRTMLGHRTDQFKDIATRLHEGAPAQPA
                     ARCALETAMIDAHCRALGIPMWALWGGSDLRERETDITIPIGTLDKTLLLAQAWYAKG
                     FRLFKMKVGADVETDIRRLEAVHRALPEVAFIGDGNQGFSRRECLAFTKSVKRFGGKL
                     VLLEQPVVREDLEALAAIRQETGIPVAADESVRSLLDAKRVVDQHAADYINIKTMKTG
                     VIEAHEIALYTLASGLKLMVG"
     gene            1000..3786
                     /locus_tag="VFR82_11990"
     CDS             1000..3786
                     /locus_tag="VFR82_11990"
                     /inference="COORDINATES: protein motif:HMM:NF023167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HEU4502361.1"
                     /translation="MKQTFRSMQLMIVLLLVLIGGWLALPSIASTILERWLERQGYED
                     VGVTVGRPGFRSLTVPRMVLTKRLTGEVVTVSLNHAQAEYTLLGLLSGRIDILTLRQL
                     TIEILASPAPYDGQPRPEEGVPDAPDSLLNAFTAGDVVQRLPFFPWDEVRLEEIKVFR
                     EQATGPLRTVMMGGTIKHKGEALVADMVLQGIDTIPYELHVTGQSAADMSLQLRAAQP
                     AATPFVLWRSQAVPKEAQVHLEGVLEVNVKELAPFLALALPIGSEWQRVGGNVTIHWT
                     GTAASGVPVSLLWKDPATEVHATVQTAASLPELKGYGKDLAVKATGTLSGNARLVHWT
                     LAGGSSATAAVSGGAVKGMERLISLAPYGSQPVRLESAQDTTGELFWAESPPRFTAAG
                     PVRISYGSEKAPIYAECVVSQIYGSGRVIDRAEAKVLMKGALAVAIQERSKHAQVTGE
                     IRGDLAWTGRTFRGTISPSSVVSFTDFKHGSLHVSGGTLQLDEPLPVEVEVSTGRWTS
                     GPGLVTWKSPYLGLGGSSLTMQRALTRVDRLEGSSNVQQAQMTATLEGLVLAQSSFRS
                     LPVDVTVRLEADPSGVKADIHHKSQHQGFKLTGQLEHQWVEGRGTLHAVVGPLTFHRE
                     SSRLGQLWTPWPYTADVTGGELAGIVDIRWATDSQQRLQIQGGSADITADRLAGRYRD
                     ILFSGLSTNVKVALEGLQRISTSRPAEVAVGSLQTGVEVTDLNMTVEGEWDVRERLPL
                     VEVRNIRCELLGGTVTSQGLRADLAYPPYGLTVLARQLDLQKILTLEQQKGLQGTGVL
                     DGSIPVTVTSRGLTVRDGSFEARPPGGVIRYMASPDATHAVTQANANMEAVLQALNNF
                     QYNVLQVGAEYIEDGTLQLKARLEGKNPDQKKSPPIHFNLTVQENIPALLKSLRLVND
                     VEDSVRKAFSGSHR"
     gene            3795..3995
                     /locus_tag="VFR82_11995"
     CDS             3795..3995
                     /locus_tag="VFR82_11995"
                     /inference="COORDINATES: protein motif:HMM:NF025003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HEU4502362.1"
                     /translation="MVRVVSIVTGGVVGLYLTLGGCTPRVEMATPDKPITINLNVKID
                     HEIRVKVDKELDEVLSRDSGLF"
     gene            4014..4349
                     /locus_tag="VFR82_12000"
     CDS             4014..4349
                     /locus_tag="VFR82_12000"
                     /inference="COORDINATES: protein motif:HMM:NF018701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HEU4502363.1"
                     /translation="MWRTYFGRIVACLVFCLSAVSPAFGISVEEAKTKGLVGERPNGY
                     LGVVNPAAPEAQALANEVNEKRRQAYEDIAKRNRTQLNAVEALAGEKAIQNTRPGYFV
                     EGPGGWTRK"
     gene            complement(4460..5104)
                     /locus_tag="VFR82_12005"
     CDS             complement(4460..5104)
                     /locus_tag="VFR82_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU4502364.1"
                     /translation="MDEPTRLTKTKEQWAKARRGGEEREVFYAGDERLPPGQHVVETF
                     PVLDLGYKPDIPLSTWTITIGGFVATPVTWTWEQFLAQPQFKNVSDFYCVTSWSRYDN
                     GWEGVSFKHILSVVKPLSLAKFVLFKSYDDYTTNLPLEACDDNDVLLTYKWNGKPLAK
                     EHGGPVRVIVPKRYAWKGAKWIKEITFSDKDEKGFWEVRGYSNSALPWDNDRYG"
     gene            5262..5822
                     /locus_tag="VFR82_12010"
     CDS             5262..5822
                     /locus_tag="VFR82_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502365.1"
                     /translation="MKTCLLVLLMIGSWWLCMTIVAMEETQAEQKSRKEGTSSGMPVK
                     SSRPPEGRQKKPLPTTTQNAQLPQPEAKPLSSAKAAKAIHDELGQAGRKPHRVIKTFK
                     KVPRKTVVVQPRMDLIHYGVLEDSQRYDPRPHAQAAGVPNPQTPELTHDHFQELDRNQ
                     DGKIDPVERAFGRIDMDRDLNTRTFK"
     gene            6114..>7493
                     /locus_tag="VFR82_12015"
     CDS             6114..>7493
                     /locus_tag="VFR82_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HEU4502366.1"
                     /translation="MSDMELSGLLPNDQADRVLAENVHPTKWVNPAADGRYNIVVLGA
                     GTAGLITAIVGASLGAKVALVEKHLMGGDCLNVGCVPSKSVIRAARAWAELRNAEQFG
                     LHIPAGVKYDFGAVMARMRKLRARISHNDSAQRYTNLGVDVFIGSGRFASADTIQVEG
                     AAGNRTLRFARAAICTGTRASAPAIPGLKEAGYLTNETVFALTELPQRIGVIGAGPIG
                     CELAQAFARFGSHVCVIEALHGILSNEDRDAAQVVEQQIIKDGVKLLCCGRDLHIVRT
                     PDGKKLSVQSHGQQFDVTVDEILVAAGRTPNVEGLGLDEAGVQFDRHGVKVNRRLQTS
                     NPKIFAAGDVCSPYKYTHAADAMAQIVVQNALFPHPFGLGYASVDSLIMPWCTFTDPE
                     IAHVGLYEKDAEAKGIGVETYTFKLDEVDRAILDGQEEGFARVHIRKGSDKILGATIV
                     AAHAGDLINE"
BASE COUNT         1628 a         2047 c         2240 g         1578 t
ORIGIN      
        1 cccgaccatg agtttcaggc cggaggcgag agtatacagg gcgatttcat gagcctctat
       61 gacgcctgtt ttcatggttt tgatattgat gtaatccgcc gcatgttggt caaccacccg
      121 ctttgcatcc aggagagacc tgacggattc atcggccgca acgggaatgc ccgtctcttg
      181 ccgaatcgca gcaagagcct ccaagtcctc tcggacgacc ggctgttcga gtaagaccag
      241 cttccctccg aagcgcttca ctgattttgt gaacgcgaga cattcccggc gtgaaaatcc
      301 ctgattaccg tccccgatga aggccacttc cggcagagca cggtggacgg cttccaatct
      361 tcggatgtca gtctcgacat ccgcacccac tttcatctta aagagacgga aacctttggc
      421 gtaccaggct tgcgccagca agagtgtctt gtccagagtg ccaataggga tggtgatgtc
      481 cgtctcgcgt tcccgtagat cggagccgcc ccacaaggcc cacattggaa tacccagcgc
      541 acggcaatgc gcatcaatca tggccgtttc tagtgcgcaa cgtgcggctg gctgggccgg
      601 cgccccttca tgcaaccgcg tcgcaatgtc cttgaattga tccgtccgat ggcccagcat
      661 ggtgcgccca accaggccga tggcagcgag gcaggaggca cggctctcgc cgcccacttc
      721 gggaaagggt gcggcttctc cgtatccttg tgtaccattg gatagggtca tacgcacata
      781 gacgttctcg gcgatgctgc gcgacccagt ggccacgaca aacggatccg tcaggggaat
      841 gtcgatgggc cagtagtcaa tttgtatgat gtctcgagtc tgtgctgaca tggccgaggc
      901 atcttaacca gaaacgagta tgatgccaat gcgaagggcc tgggctccga atcaccgccg
      961 atgtagtggg ttgatgcaga cggcgtgtga ttatccatca tgaaacaaac gttccgttcc
     1021 atgcagctga tgatcgtgct gctgttggtc ttgattgggg gatggctggc gctaccctca
     1081 attgcctcaa ccatccttga acggtggctg gaacggcagg gctacgagga tgtgggcgtg
     1141 acggtcgggc gtcccggctt tcggtccctg acagtcccgc gcatggtcct gacgaagcga
     1201 ctgacgggcg aagttgttac cgtctccctg aaccatgccc aagccgagta tacgttgctt
     1261 ggcttactgt ccggtcgcat cgatattctc accctacgac agctcacgat cgaaatcttg
     1321 gcgtcaccgg ctccttacga cggacagccg cgaccggagg aaggtgttcc ggatgcgccg
     1381 gattccttgc tcaatgcgtt cacagctggt gacgtcgtcc aacggttgcc attttttccg
     1441 tgggacgaag tgaggttgga ggaaatcaag gtgttccgtg aacaggcgac aggacctctg
     1501 cgcacggtca tgatgggagg caccatcaaa cataagggcg aagcgttggt cgccgacatg
     1561 gttttgcaag ggattgatac gattccctac gagctacatg tgaccggtca atccgcggcc
     1621 gatatgtcat tgcaactccg agccgcgcag ccggcagcaa cccccttcgt gttgtggcga
     1681 tcgcaggcgg ttcccaagga agcgcaggtt catctcgaag gggtgctgga ggtgaatgtc
     1741 aaagaactcg cgccgttttt agctcttgcg ctgccgattg gctccgagtg gcaacgggtt
     1801 ggtggcaatg tgacgattca ttggacaggc acagcggcct cgggcgtgcc ggtctccctg
     1861 ttgtggaagg accccgcgac cgaagtccat gcgaccgtgc aaactgctgc ctcacttcct
     1921 gagctcaaag gatatggaaa agatctcgcg gtcaaggcga caggcacgct ctccggcaac
     1981 gcccgactgg ttcattggac gctcgcgggt ggatcttcgg cgacggctgc cgtcagtggt
     2041 ggggcggtca aaggaatgga acgactaata agcctcgccc catatggatc gcaaccagtt
     2101 cgtctcgaga gcgctcaaga cactaccggc gagctgtttt gggccgagtc gcctcctcga
     2161 tttacggctg cgggtccagt gcgcatctcg tatggctccg aaaaagctcc gatctatgcc
     2221 gaatgtgtgg tgtcgcaaat atatggcagc ggacgcgtaa ttgatcgtgc tgaagctaaa
     2281 gtgctcatga agggggcgct ggcggtagcc atacaggagc gatcgaaaca tgcccaggtg
     2341 acgggagaaa ttcgcgggga tctggcgtgg acaggaagga cttttcgcgg cacgatcagc
     2401 ccctcttcgg tggtctcgtt cacggacttc aagcacgggt cgcttcacgt cagcggcgga
     2461 accttgcaac tggacgaacc gcttcccgtc gaagtggaag tctcaaccgg acggtggact
     2521 tccggtccgg gccttgtgac ctggaaatcg ccttatcttg gcctgggagg ctcgtccctg
     2581 acgatgcagc gggccctgac gagggtggat cgactggaag gctcgtcgaa cgtgcagcaa
     2641 gcgcagatga cggcgaccct cgaagggctt gttcttgccc agtcatcctt ccgcagcttg
     2701 ccggtggatg tgacggtgcg cctggaggcc gatccctccg gggtgaaagc ggacatccac
     2761 cacaaaagcc aacatcaggg cttcaaactc accgggcagc tcgagcacca atgggtggag
     2821 ggccgcggaa ccctccatgc ggttgtgggg ccgctgacct tccatcgaga gagttctcgc
     2881 ctgggacaac tttggactcc ctggccttat acggctgacg tcaccggcgg tgaactagcc
     2941 gggatcgtcg atatacggtg ggcaactgat agccagcagc gactacaaat acagggtgga
     3001 tcagcggaca tcaccgctga cagactggcg ggtcgttaca gagacattct cttctcgggg
     3061 ctcagtacca atgtcaaggt cgcccttgaa ggtctgcaac gcatctctac ttcgaggcca
     3121 gccgaggttg ccgttggatc gctccagact ggggtcgagg tgaccgacct caacatgact
     3181 gtcgaaggag aatgggatgt cagggaacgg cttccgctcg tggaagtacg caacattcgc
     3241 tgtgagttgt tgggcggaac ggtgacgagc caaggtttgc gtgcggacct cgcttatcct
     3301 ccctatgggc ttacggtgct ggccagacaa cttgacctcc aaaagatttt aaccctcgaa
     3361 cagcagaaag ggttgcaggg gacaggcgtg ctcgacggct ccattcctgt gacggtgacg
     3421 tctcgagggc tcacggtgag ggacggaagt ttcgaagccc gtccacccgg aggggtgatc
     3481 cgttatatgg cctcaccgga cgcaacgcat gcggtgactc aggccaacgc gaacatggag
     3541 gctgtgctcc aggcactcaa caattttcaa tacaatgtgt tacaagtggg tgccgaatat
     3601 atcgaggatg gcacattaca gttgaaggcg cggctcgaag gcaagaatcc ggaccagaaa
     3661 aaaagcccgc ctattcattt taatctcacg gtacaggaaa atatcccggc gctgttgaag
     3721 agtcttcgac tcgtgaatga tgtggaggac tcggtgcgga aagcattctc gggatcgcat
     3781 cggtaggagg gcggatggtt cgcgtggtct ccattgtgac gggaggagtg gtcggcctgt
     3841 atctcacgct cgggggatgc acgccgcgcg ttgaaatggc aacacccgac aaaccgatca
     3901 caatcaattt gaacgtcaag atcgatcatg agatccgcgt gaaggtcgac aaggagctgg
     3961 atgaagttct ctctcgggac agcgggttgt tttaaggttc agaggaggcc gtgatgtgga
     4021 ggacgtattt cggccggatc gtcgcctgcc ttgtcttctg cttgtcagca gtgtcgccgg
     4081 cgtttggcat ctccgtggaa gaagcaaaga caaaagggtt ggtgggtgaa aggccgaacg
     4141 gatacttggg cgtcgtgaat cccgcggctc ccgaggcgca ggcattggcg aacgaggtca
     4201 atgaaaaacg acgccaggcg tatgaggaca tcgccaagcg gaatcgaacg cagctcaatg
     4261 ccgtcgaggc gcttgcagga gagaaggcga ttcaaaatac gaggcccggc tatttcgtag
     4321 aaggaccggg cgggtggaca agaaaatagg attggcctcg cctcttcgtg cacgccgtgc
     4381 tctgtgagaa gtgacgctgt gttgcctcgg ttgagtctgt gcgcgagcga gaacgacgct
     4441 gaaagcctcc agagcacgtt tacccatagc gatcattgtc ccatggtaac gcgctattcg
     4501 aatagcctcg tacttcccaa aaccccttct catctttgtc ggagaacgtg atttccttta
     4561 tccacttcgc gcctttccat gcatagcgtt tcggcacgat cacgcggaca gggccaccgt
     4621 gctctttggc gagcggtttg ccgttccatt tgtaggtcaa caggacgtcg ttgtcgtcac
     4681 acgcttcgag cggtagattc gtcgtgtagt cgtcatagga cttaaacagg acgaacttgg
     4741 cgagcgagag aggtttcacc accgacagga tgtgcttgaa gctgacccct tcccatccgt
     4801 tgtcgtaacg gctccatgac gtgacacagt aaaagtcaga aacattcttg aactgaggct
     4861 gtgcgagaaa ctgctcccat gtccatgtga cgggggtcgc tacaaagcca ccgatggtaa
     4921 tcgtccaggt tgagagggga atatccggtt tgtagcccaa atccagaaca gggaatgtct
     4981 cgaccacgtg ctgtcctggc ggcaatcgct cgtcgcctgc gtaaaacact tcacgctctt
     5041 ctccccctct ccgcgctttt gcccactgct ccttcgtctt ggtcaaacgt gtcggttcat
     5101 ccattgtgag cctcctccca cagggtagga taggacggta gcgtctgaca agcggcggcc
     5161 atgtgttgcg gctcgataat cggcaggacg tgtaaatcat tgtgttcaca gagaatcctg
     5221 atcacctgct acacttaaga gcattgccgc agaggagcct tatgaagaca tgtctgcttg
     5281 ttctcctcat gatcggatcc tggtggctat gcatgacgat tgtcgccatg gaagagaccc
     5341 aggcagaaca aaagagtcgt aaggaaggaa cctcatcggg catgccggta aagagttcca
     5401 gaccgcccga aggaaggcag aaaaagcctc ttccgacgac cacccaaaac gcccagttac
     5461 cacagccaga ggccaagccg ctttcttcgg ctaaagcagc caaagcaata catgatgagc
     5521 ttgggcaggc cggacggaaa ccgcatcgcg tcatcaagac tttcaagaaa gtgccgcgta
     5581 aaaccgtcgt ggtgcagcca cgaatggatc tcatccatta tggtgttctc gaagattcgc
     5641 agcgatatga tccgcgcccg catgctcagg ctgccggtgt gcccaatccc cagacgccgg
     5701 agctcacaca tgatcacttt caggagctgg atcgcaatca agacggcaaa attgacccgg
     5761 ttgaacgggc gttcggccgc attgacatgg accgagatct caatacccgg acgttcaagt
     5821 agtctcacca gcttcttctc atctgctgag tttgccattc ttgggcgtct ccgttgactc
     5881 tgccggccgg tccaccacgc acatcatcgt ctattgatgg tgggtcgggc ggtttcgggt
     5941 gagccatcca cattgacagc cggaagagga gtccccatca ttagctggca cgtccacccc
     6001 tgtgacgtga tcgatgaatg ccacgacatc gcggctcctg cagcctacga gaagcgggca
     6061 gattacgcac ctgaaattcg ctatgatcag ccgtcatcct gagaggagca ggaatgtctg
     6121 atatggagtt gagtggactg ctgcccaacg accaagccga tcgggtcctt gcagaaaatg
     6181 tacatccgac gaaatgggtg aaccccgctg ccgacgggcg ctacaacatc gtcgtgctcg
     6241 gcgcgggaac tgccggcttg attaccgcga tcgtgggcgc cagcttgggc gccaaagttg
     6301 cgctggtcga aaagcacctg atgggtggcg actgtttgaa tgtggggtgt gtcccgtcaa
     6361 agagtgtcat ccgtgccgcc cgcgcatggg ccgagctcag aaacgccgag cagttcgggc
     6421 tccacattcc tgccggggtg aagtacgact tcggtgcggt aatggcccgg atgagaaaac
     6481 tacgggcgcg catcagtcac aatgattcag cccagcgtta cacgaatctc ggggtggacg
     6541 tgttcatcgg cagcggacga tttgccagtg ctgatacgat tcaggttgaa ggagcggctg
     6601 gaaatcgcac cctgcggttt gctcgggccg cgatttgtac tggtacgcgg gcttcagcgc
     6661 ctgccatacc cggcctgaaa gaagcgggtt acctcactaa cgaaacagtg tttgcattga
     6721 ccgaattacc gcagcgtatc ggtgtgatcg gcgccggtcc gatcggctgc gaactggcgc
     6781 aggcctttgc gcgttttggg agtcatgtct gtgtgatcga agcgcttcac ggcatcctct
     6841 ccaacgagga ccgcgatgca gctcaagtgg ttgaacaaca gataatcaag gatggcgtca
     6901 agctgctctg ttgtggaagg gaccttcaca tagtccgaac gccggacggc aagaagctgt
     6961 cggtccagtc tcatggccaa cagttcgacg tgacagtcga tgagattctc gtggcagccg
     7021 gacgcactcc aaatgtcgag ggtttgggcc tcgatgaggc cggcgtgcaa ttcgatcgac
     7081 acggggtgaa ggtcaaccgc cggttgcaaa ccagtaaccc aaagatcttt gccgctggcg
     7141 atgtgtgctc accgtacaag tatactcatg ctgccgacgc aatggctcaa attgtcgttc
     7201 agaatgcgtt gtttccacac cctttcggcc tcgggtatgc cagcgtagac tcactgatca
     7261 tgccgtggtg caccttcacg gaccctgaga ttgcacacgt cgggctgtat gaaaaggatg
     7321 cggaagccaa ggggatcggc gtggaaacgt acacgttcaa gctcgacgaa gtggatcgtg
     7381 ccatccttga cggccaagag gaagggttcg cccgggtgca catccgaaag ggcagcgaca
     7441 aaatcctggg cgcgacgatc gtggctgctc atgccggaga ccttatcaat gag
//
LOCUS       DASTQY010000368         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_277483, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000368 DASTQY010000000
VERSION     DASTQY010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277483"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1623)
                     /locus_tag="VFR82_12020"
     CDS             complement(199..1623)
                     /locus_tag="VFR82_12020"
                     /inference="COORDINATES: protein motif:HMM:NF024839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEU4502367.1"
                     /translation="MDELEQSGHDDPHTRTSNGKQNFARYMIGGLCLILFLALTGVGY
                     VQVEERRGGGLRPFVSAENKKCIDCHMAKDVGIGGINDWKTSRHAPKGIGCVECHRAE
                     KGEADGYDHEGFLISTLVTPKDCMRCHDREAKEFAKSHHAKAAQFTGSLDNFLGNVVE
                     GPEVVTTGCAGCHGSVVKVMEKGKLHPATWPNSGIGRVNPDGSKGTCSACHARHSFSI
                     AQARQPESCGRCHMGPDHPQIEAYYESKHGVMYEANKEKMKLADPTEKWHPGKDYLYP
                     TCATCHMSATGTQEVTHDVGDRISWTLRPVISTRLENYEDRRKSMKQICSACHSQQIV
                     DRFFTSMDQGINLYNDKFGKPAKEAMDKLLAMKKLTPTPYDEKIEWVFYELWHHEGRR
                     ARHGLSKVAPDYVHWQGFYEVAKSFYTKFLPLVRELSPQVAEELLRNESHRWIEKGMS
                     KEEIAQMLEFYNKEMQGKRGGVDG"
     gene            complement(1638..>2731)
                     /locus_tag="VFR82_12025"
     CDS             complement(1638..>2731)
                     /locus_tag="VFR82_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEU4502368.1"
                     /translation="ISQAHKSNPHAHQIPHCVVDEMDSATTRSIDPKSGGKTSWIDYI
                     QKDLPQHLDWWPYTLGAIPLTLFGILVATGLLLTFYYVPSPEKAYESVDQITNEIYLG
                     WFVRGLHKTSVDLMILFLLFHVIRVFITRAYRTPGELKWVSGSIVLFVTLAMGFTGYS
                     LIFDNVSYWGMTVVTNMVGTLPVIGTPLLHLLRGGEEVSGVTLLRLYDLHTKLLPVLL
                     GGLVLGHVLVVRLLGFADVRGSRGFHPFYPEHTLKMGVVAVGLLLLIVNIAMIFPPLL
                     GPPANPQEVASDVSPPWYFSAPFMWITLLPGPVALWSLVGFAGLFVIYPFLDRMLAER
                     GWPMDVVNAIAAAAVVGAMVFLMYLDTQM"
BASE COUNT          554 a          823 c          771 g          583 t
ORIGIN      
        1 cttgcgggag agaaacggcc gtagcttgat cagttgttgt ttgaaccgct ccaggtagcg
       61 atgaagaatg tcggtacgcg ttaatcccgt gcgaactcgt ttcggcattg catccctccc
      121 atggcgggac gagaatagcg caacgcagaa gtttgctcaa gaggagcagc aagccgtcgc
      181 agcatcttca cccgcggcct acccgtccac tcctccgcgc tttccctgca tttccttgtt
      241 atagaattcc aacatctggg cgatttcctc cttgctcatg cctttttcga tccaccggtg
      301 gctctcattc cgaagcagct cttcggccac ctgcggcgac aactcccgta cgagcgggag
      361 gaacttcgtg tagaaactct tggccacctc gtagaatccc tgccagtgaa cgtagtcagg
      421 cgccaccttg gagagcccgt ggcgtgcacg acgcccctcg tggtgccata gctcgtagaa
      481 cacccactcg atcttttcgt cgtatggggt cggcgtgagc tttttcatgg ccaagagctt
      541 gtccatggct tctttggccg gcttgccgaa tttgtcgttg tacaggttga tgccctggtc
      601 catggacgta aagaaccggt ccacgatctg ctggctgtga caggccgaac agatttgctt
      661 catggatttc cgccggtcct cgtagttttc gagacgcgtc gaaatgaccg gccgcagcgt
      721 ccaactgatg cggtctccca catcatgcgt cacctcttgc gtcccggtcg cgctcatgtg
      781 acaggttgca caggtcggat agagatagtc tttgcccgga tgccacttct ccgttgggtc
      841 cgcgagcttc atcttctctt tgttggcctc gtacatgaca ccgtgcttac tctcataata
      901 ggcctcgatc tgcggatgat ccggccccat gtggcagcgg ccgcaacttt cgggctgccg
      961 cgcttgagcg atggagaaac tgtgtcgcgc gtgacaagcg gagcaggtgc ctttcgagcc
     1021 gtcgggattg acccgcccga tccctgaatt gggccaggtc gccggatgga gcttgccctt
     1081 ttccatgacc ttcaccacgc tgccgtggca gccggcgcac ccggtcgtca cgacctcagg
     1141 tccttcgacg acgttgccca gaaaattgtc gagtgacccg gtgaattggg ccgccttggc
     1201 gtgatgggac ttggcgaact ctttcgcttc ccggtcgtgg caccgcatgc aatcctttgg
     1261 ggtgaccagc gtcgagatga gaaagccctc gtgatcatac ccgtccgctt cgcctttttc
     1321 ggcccgatga cattccacac agccgatgcc tttcggtgca tggcggctgg ttttccaatc
     1381 gttgatgccg ccgatgccca cgtctttggc catatggcaa tcaatgcatt tcttgttctc
     1441 cgccgataca aacggacgga ggcctccacc ccgccgctct tcgacctgga cgtagccgac
     1501 ccccgtcagg gcgagaaaca gaatcaggca gagtcccccg atcatgtagc gggcgaagtt
     1561 ctgcttgccg ttgctcgtgc gcgtgtgcgg atcgtcatgg ccgctttgtt ccagctcgtc
     1621 catcagctcc tcctggttca catctgcgta tcgaggtaca tcaagaagac catggcgccg
     1681 acgacggcgg cggcagcgat ggcattaacc acgtccatcg gccatccacg ctcagccagc
     1741 atgcggtcaa ggaacggata gatgacaaag agcccggcaa accccactaa gctccaaagt
     1801 gccaccgggc ccggcagcaa cgtgatccac atgaacggcg ccgaaaaata ccagggcggc
     1861 gaaacgtcgg aggcgacttc ctggggattc gccggcggcc cgagcagcgg cgggaagatc
     1921 atcgcaatgt ttacgatcag gaggagcagt ccaacggcca cgactcccat cttgagcgtg
     1981 tgctccggat agaaggggtg gaagccgcga cttccgcgca cgtcggcgaa tcccagcagg
     2041 cgcacgacga gcacgtgacc gaggaccagg cctcccagca gcaccggcag taacttcgta
     2101 tggaggtcat acaacctcag caaggtaaca cccgacacct cttcaccccc gcgcagcaaa
     2161 tgcagcagtg gtgtgccgat gacgggaagc gtaccgacca tattcgtcac gaccgtcatg
     2221 ccccaatacg agacgttgtc gaagatgagc gagtaaccgg taaaccccat ggccaaggtg
     2281 acgaatagga cgatcgatcc gcttacccat ttcaactctc cgggtgttcg atacgcgcgg
     2341 gtgatgaaca cccggatcac gtggaataga agaaacaaga tcatgagatc cacggacgtt
     2401 ttgtgcagac cccggacaaa ccagcccaga tagatctcgt tggttatttg atcgacgctc
     2461 tcgtaggcct tttctggaga aggcacgtaa tagaacgtca gcagcaggcc cgtggcgacc
     2521 aagatgccga agagcgtaag cgggatggcg ccgagtgtgt acggccacca gtctagatgc
     2581 tgcggcagat ccttctggat gtaatcgatc cacgaggtct tgccgccgct cttcgggtca
     2641 atgctccggg tggtcgctga gtccatttcg tctaccacac aatgaggaat ctgatgcgca
     2701 tgagggttcg acttatgcgc ttgcgatatt g
//
LOCUS       DASTQY010000369         3397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1284821, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000369 DASTQY010000000
VERSION     DASTQY010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284821"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFR82_12030"
     CDS             complement(<1..352)
                     /locus_tag="VFR82_12030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4502369.1"
                     /translation="MSLPNQLIIRAANLDDVESIITFSAAMALETENRQLDLARLREG
                     TLSLLNTPSYGFFMVAEIRDGEQRRLIGQLMITYEWSDWRNGVFWWIQSVYVDPVWRR
                     RGVFRRIHEAVASQA"
     gene            complement(358..972)
                     /locus_tag="VFR82_12035"
     CDS             complement(358..972)
                     /locus_tag="VFR82_12035"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEU4502370.1"
                     /translation="MRTLSRYFFTGLLILVPTWATFLILSALLSAVDRMIAALLGQVG
                     VIATPVIGLLLFCLLVITAGALATHVLGSQIIASTERWIAQIPLVNSIYLTIKSMTDL
                     FQFRSRFGRSTVIAFPFPRDGVWALGFVMGVAPPAVQVERSVNLLMVFVPTAIHPFTG
                     YLAFVPQPQIRPLNLLAEDAMKMEFSAGFYKPAEGWLGAPKITT"
     gene            1114..1404
                     /locus_tag="VFR82_12040"
     CDS             1114..1404
                     /locus_tag="VFR82_12040"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEU4502371.1"
                     /translation="MADPSSTRRPVQVDKDLLAILCCPETKLDVSLADDALISKVNDA
                     VGRGVLKNKAQKPVTELLDGGLIRADKKILYPIREDIPVMLIEEGIPLDQVL"
     gene            complement(2069..2440)
                     /locus_tag="VFR82_12045"
     CDS             complement(2069..2440)
                     /locus_tag="VFR82_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502372.1"
                     /translation="MNSTLQRAVTSFYNLVYEAQTFATTMARIDTAEQEHYAGRIEGL
                     NWVLDRCQELEDMDTNLTPSSLQRVLTEVKSDLDHELSVQRREKGRRADGREEALNFV
                     ADYLSSLITATEIESAKTPAA"
     gene            complement(2527..2856)
                     /locus_tag="VFR82_12050"
     CDS             complement(2527..2856)
                     /locus_tag="VFR82_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502373.1"
                     /translation="MTSGQDQSIPFAAQAIPFDEFLAVGKLPEGYLTSEYVAQQFVER
                     LVHYILSVPSGSYTMAQLSHLLEQLDPRAQVFFFKRLKETSPDSLKDFAPLYYGFMNE
                     FHSLLFT"
     gene            complement(3139..>3397)
                     /locus_tag="VFR82_12055"
     CDS             complement(3139..>3397)
                     /locus_tag="VFR82_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate oxidoreductase subunit beta"
                     /protein_id="HEU4502374.1"
                     /translation="NAIEKYLVPSRELLAVLQLWRASQQIVFRYDVIPGPKVFETQIH
                     GKRFEMYNDTVLGFNKSGKEVARIQVEEPIYVRPAERVNWL"
BASE COUNT          792 a          921 c          968 g          716 t
ORIGIN      
        1 ttgcttgaga cgcgactgcc tcgtggatgc gccgaaagac gcctcgcctc cgccacaccg
       61 ggtcaacata gacgctttga atccaccaaa agacaccgtt gcgccaatcg ctccactcat
      121 acgtaatcat gagttggccg atcaacctac gctgttctcc gtcgcggatt tctgccacca
      181 tgaagaatcc gtacgatggt gtgttgagca gcgacaaggt cccttccctg agcctcgcca
      241 ggtcaagctg cctgttctcg gtttcaagtg ccatggctgc gctgaacgtg atgatggatt
      301 ccacatcatc cagattggct gctcgaatga tcagttggtt cggcagggac atgagggcta
      361 tgtcgtgatt ttaggggctc cgagccagcc ctctgccggc ttatagaagc cggcagagaa
      421 ttccattttc atggcgtctt ccgcgagcag attaagcggg cggatttgcg gctgggggac
      481 aaatgcgagg tatccggtga atgggtggat ggcagtaggc acgaagacca tcaacagatt
      541 gacggatcgc tcaacttgaa ctgccggcgg cgcgacaccc atgacgaatc caagagccca
      601 cactccgtcg cgcggaaacg ggaaagcgat gaccgtgcta cgtccaaaac gcgatcggaa
      661 ttggaacagg tcggtcatgc ttttgatggt gaggtagatg ctattcacca gaggaatctg
      721 ggcaatccat cgctcagttg atgcgatgat ctgactcccc aatacatggg ttgcgagtgc
      781 gccggccgtg atcaccagaa ggcagaaaag cagcaaaccg atgacaggag tggctatgac
      841 gccgacctga ccaagaagcg ccgcgatcat gcgatcgacg gctgataaca gcgcggacag
      901 gatgagaaat gtggcccaag tcggtaccaa gatcaagagg ccggtaaaaa aatagcgcga
      961 cagtgttctc atggtctttg tgattcagac ggcagatggt cgagcggcag tgtaacagat
     1021 gcgagtggta attagagcgt gggcacactt gatctcagcc ggagggtgca ttatcctggg
     1081 actcacccgc aatcttaaat cagaggtgtg gccatggctg atccttcgag cacgaggcgt
     1141 ccggtccagg tagacaagga cttgctggcc atactgtgtt gccccgagac caaactcgat
     1201 gtcagcttgg ctgacgatgc tctgattagc aaggtcaacg atgccgtggg ccgaggcgtg
     1261 ttgaagaaca aggcccaaaa gccggtgacc gaattgctcg acggcggctt gatcagagcc
     1321 gacaagaaga tcctgtatcc tattcgtgaa gatatccccg tgatgctcat cgaggaaggt
     1381 atccctctcg atcaagttct ctagcgactc acgccgatcg tcagtttgaa gccacggctt
     1441 acggctggaa catacctcgc tgttctctct cagtcttcga tccggttgag gatccgcatt
     1501 gctggcagag gtattcatag aggttgccgg tcggtaagac gagaagcagg cgttcgcgcg
     1561 tcggagtggc cacacgacag cgcggacagt acagcaggga tgcattcagg tttttgaact
     1621 gatcctcttg ttcagaggta cggggctttc cggctctcgg agggccagga ggccagccgg
     1681 ggcctggtgg tttgcgcgga ggcagtggca tacgatggat tgtaggggag cggttaccct
     1741 cggtcaatgg tgctgctgtc cacgaggcat gggatgtcgt ctaccaagcg tcgcttgatc
     1801 caggtccacc attccgccga gaccgaaaca gcctccacac acctcttcct ccctgcacca
     1861 ccgcataaat acctatcccg ctgagcagcc catagaaccc ggccgtgctc cacggccaca
     1921 attcaggaga atgcagcatc accccgaggg cgacatggcc gcctgccccc aagcacagag
     1981 cgaaaatgat ccactcgcgc atcacgtgaa gcaacccggc atgcaaccag actggaaggg
     2041 ggatgacgga gtccccaatg gcggagcact aggcagctgg agtcttggcc gattcgattt
     2101 ccgttgccgt gatgaggctc gacagataat ccgccacaaa attcaaggcc tcttcgcggc
     2161 cgtcggcgcg acggcctttt tcccgtcgct gaaccgaaag ctcgtggtcg aggtcggatt
     2221 tgacctcggt gaggaccctc tggagtgacg atggagtcaa attcgtatcc atgtcttcca
     2281 gttcttgaca acgatccagc acccaattga ggccctcaat ccggcccgcg tagtgttcct
     2341 gttcggcggt atcgatccgc gccatagtgg tggcgaatgt ctgagcttcg tacacgaggt
     2401 tataaaaact ggtgacggct cgctgaaggg ttgaattcat agaactcctt tatattccag
     2461 tgcgatcgag ggatcatatg attatacatg agaatacctt gccgacaagc agaaattttg
     2521 cagggcttac gtaaaaagaa gggagtggaa ctcattcatg aaaccataat agagcggggc
     2581 aaaatcttta aggctgtccg ggctggtttc ctttaatcgc ttaaagaaaa aaacctgtgc
     2641 tcgtgggtcg agttgctcca gtaagtggct cagttgcgcc atcgtatagc tgccggaagg
     2701 cacgctgaga atgtagtgaa ccaggcgctc gacgaactgt tgcgccacat attcactcgt
     2761 caagtatcct tcaggaagct tccctacagc caggaactcg tcgaacggta tcgcctgtgc
     2821 cgcaaatggg attgattggt cttgacccga ggtcacgctg cacgcacctc acggaagtcc
     2881 tagaaatatt acgacaactc tactggaccc cccctacccc gtcaagcggg cagaagcagc
     2941 atacgccctg aggcaaaaga ccaataaacc agaaggggca ggggtccctt ggggaaccct
     3001 gcctacctat tgaggcgaac aggtagaacc tcacccatca tttggttgtc tacccgtatt
     3061 tggaggttgt cagaaacaga aagaggcagg gctccctgtg gaaccctgcc cccttcatga
     3121 agcgtcccta gctgtcgatt acagccagtt cacgcgctcg gccggccgaa catagatcgg
     3181 ctcttcgacc tggatacgag ccacttcctt gcccgacttg ttgaagccca gcacggtgtc
     3241 gttgtacatc tcgaagcgct taccgtggat ctgggtttcg aacactttcg ggcccggaat
     3301 gacgtcgtac cggaagacga tctgctggct ggctctccag agctggagga cggccaacag
     3361 ttcgcggctg ggcacgaggt acttctcaat ggcattg
//
LOCUS       DASTQY010000370        16716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1223124, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000370 DASTQY010000000
VERSION     DASTQY010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 16716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16716
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223124"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1771)
                     /locus_tag="VFR82_12060"
     CDS             complement(<1..1771)
                     /locus_tag="VFR82_12060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEU4502375.1"
                     /translation="MTTTASRPTLMGTATDDPFSLLAKEWLVTNGIGGYASSSLLGVA
                     TRRYHGLFVPDLPGRGRTLLIPRLDETVDQGTGMALLSGAEYADGRIEMDGIRYLKDI
                     RWEWQTPIWIFDMGHAVLEKRIIAPHGQNTIYIQYTSLSGSMRLHLRPYLTYRMHDAR
                     LGESDAGSFTMTIDSGRYEIMLPEGVPTLKLCVRPRGGVFVADEVTSEGVSYRVDRDR
                     GSEHIQDLFSPGYFTVDLSDGQCVALVASVESWDLLNFDSNDIVRAEQRRLEHLVSLA
                     PALQQDDLSKRLQLATDQFIVQPGARPEEQAIAQASGDDARTVIAGYHWFGDWGRDTM
                     ISLEGLTLSTGRQQETRAILRTFARYIQDGLIPNLFPEGSREALYHTADATLWYFHAL
                     DRYLSVTDDRETLALLYPLLEEVVHHHRKGTCFNIGVDKRDGLLRAGAPGYQLTWMDA
                     KVDDWVVTPRRGKPVEIQALWYNALRLMNQWGSQLGAKTDLWLQMAEQAEGSFHRRYW
                     IDSGGYLFDVVDGERGDDPSLRPNQIFSLSLSFPILRPERWRAVVDMVSERLLTPFGL
                     RTLASDHPDYKPSYSGDLRARDAA"
     gene            complement(1780..3693)
                     /gene="treZ"
                     /locus_tag="VFR82_12065"
     CDS             complement(1780..3693)
                     /gene="treZ"
                     /locus_tag="VFR82_12065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEU4502376.1"
                     /translation="MPTSSTISGRTPTTASQTSWHMTLGAQVETGGVRFRVWAPKRAR
                     VDVVLEEGGRSFPLMKDEAGYFSGLVPIATGGMRYRYRLDNGEAYPDPCSRYQPEGPH
                     GPSLIVDPSTYTWRHPEWQGVRMQGQVIYELHVGTFTPEGTLDATIAQLDELKDCGVT
                     LIELMPLAEFPGRWNWGYDGVDLFAPAHVYGDPDALKRFVDAAHERGLGVILDVVYNH
                     FGPDGNYLPAYSDLYLTDRHPNEWGQAINFDGHGSGPVREFFIQNACYWITEFKLDGL
                     RLDAVHAIYDDSPVHVLAELSRQARVAAGARSIVLIAECETQLITTIQSIEDGGWGLD
                     GVWSDDFHHISRVALTGRGQAYYSDYRGTAQELLSVIKRGFVYQGQRYEWQKKPRGTV
                     VKNEPAPGFVFYLQNHDQVANHLHGDRIHALASHARYRAIAALLLLAPETPMLFMGQE
                     FGASNPFLFFADHHSDLAAKVYEGRKKFLAEFPEYATPEAQAAVPDPADEATFQRSCL
                     DLSERTRHAPIYRLHKDLLRLRRDDAILARQDRRLLDGATLGPQALALRFLGPADDDR
                     LLVVNLGPDLNFIPAPEPLLAPVREGSWTLQWSSEHPLYGGPGIVNPLSEQGWRIPSA
                     TATLFRSVRKDPS"
     gene            4033..4512
                     /locus_tag="VFR82_12070"
     CDS             4033..4512
                     /locus_tag="VFR82_12070"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HEU4502377.1"
                     /translation="MRKQNWHTILIGGVCMIASGCITDATTELTKAPFDATSQLTNGT
                     TAALSEFTDPLSDFTSSTTPGAEGGIVPSRAKKRLQIFTASSYENLRADISRGHGEYL
                     VSLAKLAGVPSGQFRQFQTFMQDSYSTMFEKEIPIPESAHQIVEIAWAAGYGSSVTR"
     gene            complement(4577..6751)
                     /locus_tag="VFR82_12075"
     CDS             complement(4577..6751)
                     /locus_tag="VFR82_12075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502378.1"
                     /translation="MSNTITVVEINYEDDVVTARQRARQVSRLLTFEEQDQTRISTAV
                     SEIARLFVHSKRPALIEFLIEGTTVPQLLLVNLGWSRGGSAKSGPPNGRNLDPEWESA
                     VTSARRLMDHGEVRTDSPEGSLIRLVKILPHRAPLFVGKALERVIKQLGKQEPQNPME
                     EVQQQNQELLRALEELHERQQELVRLNGELEDTNRGVVALYAELDEKAGHLRRADEMK
                     SRFLSNMSHEFRTPVNAILALSHLLLERTDGELNSEQEKQVGYIRKSGEDLLELVNDL
                     LDLAKIEAGKITVKVSEFEVATLFSALRGMLRPLLVTEAVNLVFEEPSGIPLMNTDEG
                     KVSQILRNFVSNAIKFTPRGEVRVTAALADDGRAVAFSVADTGIGIASEDQNRIFEEF
                     SQVDHPLQLQVKGTGLGLPLCRKLANILGGSITVTSRIGAGSTFVATIPIHYSVNTIE
                     LAEQIEPVQVRPDDFRLPILVVEDEPETRLLYDKYLRNTAFRPIAAGSIRQAREQLRR
                     QLFAAIVLDVLLPDESAWQWLAELKSQEATKKLPVLIVSSVEDPRKAMALGADDYCVK
                     PLRRIWLLDRLHRVTGIVREPSAGPPLVLVIDDQEADRYILRHYLEDFKCSIVEATGG
                     EDGLKLARQLKPALILLDLNMPKIDGFEVLAQLKANPETASLPVVIVTSHVLAPEQTT
                     ALGHARAVLMKHELSSAAWQRLFQEIGLTSIEPAVPETLKPS"
     gene            complement(6754..7806)
                     /locus_tag="VFR82_12080"
     CDS             complement(6754..7806)
                     /locus_tag="VFR82_12080"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding SpoIIE family protein phosphatase"
                     /protein_id="HEU4502379.1"
                     /translation="MEVIGLATDRQVCVPVTEQSQPAAARRAAVSLAQEAGIDEPTIG
                     HVALVVTELATNLVKHAQGGELLIRCLGPEGYEGIEVLSLDKGPGMSNVSRSLEDGYS
                     TAGSPGTGLGAVLRTAEQFDVYSQPGKGTALVARVRSRQGGREESQAQAVGVVRQAKP
                     GEAVCGDNWIVRWSADGWLCAVADGLGHGSIASQAATAIVEAVRRAPANRTPVELVEV
                     AHQAAKPTRGAAVGIAVLDGSKGLLRFAGIGNIAALVVNGTERRHLVSHNGIIGHDYR
                     KVSEFTQPWHAQSLLLLHSDGIATHWDLDRYPGLLSRDPSLIAGILYRDFKRGRDDAT
                     VVVIRQHPSAGVPSSE"
     gene            complement(7797..8201)
                     /locus_tag="VFR82_12085"
     CDS             complement(7797..8201)
                     /locus_tag="VFR82_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010883997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEU4502380.1"
                     /translation="MAVLRDELIALRSTDDIIKARQLVRDCAVLQGLSLVDQTKLVTA
                     ASELARNTLVHGGGGEMCLQAVHEGTRRGVQVTFTDQGPGIADVELAMTDGYTTRGGL
                     GLGLSGSKRLVNEFTIESKPGKGTTVRIVRWK"
     gene            complement(8182..8565)
                     /locus_tag="VFR82_12090"
     CDS             complement(8182..8565)
                     /locus_tag="VFR82_12090"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU4502381.1"
                     /translation="MERIPILKMGPYLLVTIQVDMHDRVAMTLQDELTERIVAERARG
                     VLLDISSVDVVDSFIGRMIGNIASMSRVLDAETVVVGMRPAVAITLVELGLALPSIKT
                     ALNVDAGMALLKSAGKMSGNGRLTR"
     gene            complement(8613..9476)
                     /locus_tag="VFR82_12095"
     CDS             complement(8613..9476)
                     /locus_tag="VFR82_12095"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU4502382.1"
                     /translation="MSQNSQMADEIRNRQDLILKHWMEYQLSALTLRRDLVKESDLRE
                     MSRRFLDLFSEAFSVSSDPFSPSWKPVKEHLAEITRTRAEQGFSPSETATFVFSLKQP
                     LFDLAQQRAGQDTKKLAETLWDLTALLDKLGLYTTEAYQETRESVIMRQQQELMELST
                     PVVQLWDEVLALPLIGTLDSARTQVVMENLLQQVVETGARIAIIDITGVPTVDTLVAQ
                     HLMKTVAATRLMGADCIISGIRPQIAQTIVHLGVNLNDIVTKATLADAFMIALQRLGA
                     TVTRNAAATSG"
     gene            complement(9581..11362)
                     /locus_tag="VFR82_12100"
     CDS             complement(9581..11362)
                     /locus_tag="VFR82_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502383.1"
                     /translation="MATLRVLIVEDDATDAELVVRELKKSGFEPESRRVDTRDAFIIA
                     LDESTPDVIIYDHNLPHFSSSEALELTRQRDQAIPFIIVSHAIGDEDAVALMRAGAAD
                     YLLKDRLGRLGEAVRHALEQRRLREEHTAARNAIVTLNADLELRIASRTAQLKVANHA
                     LEMELGQRKRAEEALRRLNGDLEQRVTERTRDLVASNQRLQALATELTLAEQRERKRL
                     ASELHDYLAQMLALGRIKVTQLRQKLSGQAPAAQQLVAEVDNLLEKAGEYTRSLMAKL
                     NPPVLDELGLPSALTWLAKQMPLHGLLVEVRLSTEQVPLPDDQSVLLFQSVRELLINV
                     AKHAGTDRATVSLKVDQEQQLFIEVQDSGRGFDPAAMEAKSEKEHFGLFSVRERMQAM
                     GGRLEVKSMPEQGTTVTMMLPLVKAKTADHAGKTSEVAATAARPKQESEQSIYRIPHA
                     PFHPPPGNTIRLLLVDDHTMVRQGLRSLLESYEDVVIVGEGSNGEEAIELVEQLRPAL
                     VVMDINMPKKNGIEATARIKARFPETVVIGLSVQAGEEARRAIVNAGGAVLLTKEAAV
                     DELYRTIREVLDAQPANSSREGVAEVR"
     gene            complement(11383..11853)
                     /locus_tag="VFR82_12105"
     CDS             complement(11383..11853)
                     /locus_tag="VFR82_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502384.1"
                     /translation="MTNGTEILLIEDNPEDVEITLRAFHKHHIANKIHVVRDGEEALE
                     CLFGTGRYAERSPCRNTKLILLDLKLPKVDGLEVLQRCKSDPRTKGIPIVVLTSSREE
                     QDLVKSYNLGVNSYVVKPVDFPQFSDAVRQVGLYWVLLNQFPSELALESASQSN"
     gene            complement(11850..14954)
                     /locus_tag="VFR82_12110"
     CDS             complement(11850..14954)
                     /locus_tag="VFR82_12110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4502385.1"
                     /translation="MTCTPEFGGQMKPSGLTAVIREHIVALFATILALGCRLLLDPYL
                     GDHLPYATFFVSIAFTTWYAGVSASLTATLLGGFAAVWFFVPPRFSLDMTDLSQQVGL
                     VLYGVVGLTLIAVGQVMHRARRRAEELAQGLRVTEERLTLAQQASQVGSFDWNLDTGV
                     NSWSPELYAMYGLRPDDFGKTQAAWESCLHPDDRHEMVEAVERSRRSGDPEDREFRIV
                     KPNGEIRWLAGRWRWLRDRAREPVRLTGVNFDITERKHDQEALRLSQSRYRELIQSLP
                     AAVYTCDAQGRIQLYNQAAVSLWGREPEIGKDLWCGSWKIYRPDGTPLPLDECPMAVT
                     LREGRAVRGEEIVIERPDGSKRHVVPHPDPICDASGTVIGAVNMLIDVTEQKRIDDHL
                     RESEERTRAIFESSLDALITMDADGRIQDFNPAAEKTFGYTQAHALGRSVADLIIPPR
                     LRESHQVGLRRYLETGEASVIGRRIQMPALRADGTEIPVEFSIAVTRRPGRPPFFTAY
                     LRDISDRQRAERATAHLAAIVASSDDAIVSKNLQGVVTSWNRAAERLFGYRADEMIGR
                     PVTVVIPADRQDEERRILEEISRGRTVEPYETVRRRKDGSEVHVSLTISPLFDTHGKV
                     IGASKIARDITQRVEQDRALKESRERLRHALAYQEAVINNMGEGLYTVNRHGLVISIN
                     HAAEKLFGWSKEELLGLKMHDVTHYKHRDGTPFPAHDCAGFQVLQKGTPLINYPDVFV
                     RKDGTCFDVVYSAMPIRAGEEIAGLVVVFRDVTEEKRAEQALRDRDRALTMANDELNQ
                     QKSGLADANKELQSFSYSVSHDLRAPLRTIDAYIRILEEDHGAHLNSEARRCLGIVRK
                     AAGQAGELIDDLLEFSRLGRIGMDFRPTKMVEIAQEVAGELKVTDDGRTVDLTIGDLP
                     PCHGDWRLLKLVWTNLLANAFKFTRHRPVAQIEVGWLPDDRQADALVYFVRDNGVGFD
                     MKYVHKLFGVFQRLHLKEEFEGTGVGLAIVQRIVQRHGGRVWAEGKVDGGATFFFSLR
                     KALP"
     gene            complement(15128..15439)
                     /locus_tag="VFR82_12115"
     CDS             complement(15128..15439)
                     /locus_tag="VFR82_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502386.1"
                     /translation="MNAIALFLMLVLNGNMTCGAEPPDQTMLGDQVVQVQVFDCYWSE
                     RESASHQFVVISPVCPTYVAQPILLKEVHAHKGWVMNRFGEFYPATTNIEMMDVYRPP
                     C"
     gene            complement(15779..15937)
                     /locus_tag="VFR82_12120"
     CDS             complement(15779..15937)
                     /locus_tag="VFR82_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502387.1"
                     /translation="MKWIGGRADESQTQSHGTDFVVGLWVKGRWEALSSSQLIVTDST
                     DLPHRENP"
     gene            15937..>16716
                     /locus_tag="VFR82_12125"
     CDS             15937..>16716
                     /locus_tag="VFR82_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU4502388.1"
                     /translation="MTGLFILALVWFLHQAQDFAVPLTLALLLHFLFMPIVRWFKRHH
                     VVEPVAAAIILLAMTGLVTITMYELAAPAAQWLEKAPRAFRVIESKLLPVKESVSQLS
                     EATEAMNRVGQTDSSPAVRKVEVVPDRRAAVVSLLGKTTAGMGTTLLLLYFLLASGDL
                     FAKKLLTVLSRREDRGCAADILEGLERQMSQYLFQTVVIQSGFGFILWLSLWTLGMPN
                     PGLWAAAAAILQIVPYIGGLAVLVLIAVVAAVTFDNTWSIAG"
BASE COUNT         3396 a         5090 c         4650 g         3580 t
ORIGIN      
        1 acgcggcgtc acgggcacga agatctccgg aatagctcgg tttgtagtcc ggatggtccg
       61 aggccagggt gcgcaacccg aagggggtca acagacgttc actgaccatg tccaccaccg
      121 cccgccatcg ctcgggacgg agaatgggga agctcagcga aagggagaaa atctgattcg
      181 gccgaaggct cgggtcatcg cccctttcac cgtccaccac gtcaaagagg tacccgcccg
      241 agtcaatcca gtagcggcga tgaaatgagc cctccgcctg ttcggccatc tgaagccaga
      301 gatcggtctt tgctccaagc tgactccccc actggttcat caatcgcaac gcgttatacc
      361 agagcgcttg aatttcgacc ggttttccgc gacgaggcgt aaccacccaa tcgtcgacct
      421 tcgcgtccat ccaggtgagc tggtatccgg gtgctccggc acgcaacaac ccgtcacgtt
      481 tgtctacgcc gatattgaaa caggtgcctt tgcgatggtg atgcacgact tcttccaaca
      541 ggggatagag caaggcaagg gtttctcgat catcggtaac cgaaagataa cgatccaggg
      601 catggaaata ccagagtgtg gcgtcggctg tatggtagag cgcctcccgt gatccctccg
      661 gaaacaggtt gggaatgagt ccgtcctgaa tgtagcgagc gaacgttcgc aggatggctc
      721 gtgtttcctg ttgcctgccg gtagacagcg tcagcccctc caggctgatc atggtatcgc
      781 gtccccaatc gccgaaccaa tggtagccgg cgatcacggt ccgcgcatca tcgccggagg
      841 cctgggcgat ggcttgctct tccggtcggg ctcccggctg gacgatgaat tgatcggtgg
      901 ccagctgaag tcgcttactc aagtcgtcct gctggagcgc cggggcaaga gacaccagat
      961 gttcgagacg acgttgctct gcccgcacga tgtcgttcga gtcgaaattc agcaagtccc
     1021 aggattcgac actggcaacc agcgcgacgc attgaccatc tgataggtca accgtaaagt
     1081 aaccgggact gaaaaggtct tggatatgct cagagccgcg atcgcggtcg acccgataag
     1141 acacgccttc gctcgtgact tcgtccgcga caaagacacc gccgcgcggc cgcacgcaca
     1201 atttgagcgt aggaactcct tccggcagca tgatttcata gcgcccgcta tcgatcgtca
     1261 tagtgaacga cccggcatcg ctctcgccca gtctggcgtc gtgcatccga taggtgaggt
     1321 agggccgtag atgcagcctc attgatccgc tcaacgacgt gtattggata tagatggtgt
     1381 tctgcccgtg cggtgcgatg atccgttttt ccaacacggc atggcccatg tcgaaaatcc
     1441 agatgggcgt ctgccattcc catctgatgt ccttcaagta ccggatgccg tccatctcaa
     1501 tccgtccgtc ggcatattca gcgccggaca gcagcgccat tcccgtgccc tgatccaccg
     1561 tttcgtccaa acgcggaatg aggagggtgc gtccccgacc aggcagatcc gggacgaaca
     1621 ggccatgata acgacgcgtc gccacaccga gcagcgaact tgacgcatat ccgccgatgc
     1681 cgttcgtcac cagccattcc ttggccagta acgagaacgg atcatccgtc gcggtaccca
     1741 tgagcgtggg tcttgaagcc gtggtggtca tggtcgcaat cagctcgggt cctttctgac
     1801 tgatcgaaac agcgtcgccg tggcagaggg tatgcgccaa ccctgttcgc tcaacggatt
     1861 gacgatgccc ggcccgccat agagggggtg ctcactcgac cattgcaagg tccaggatcc
     1921 ctcgcgcaca ggggcgagga gcggctccgg agcaggaata aaattcaggt ccggtccaag
     1981 attgaccact aacaatcgat catcatcagc tggtcccagg aatcggagtg cgagagcctg
     2041 cggacccagc gtggccccgt caagcaagcg tcgatcctgc cgtgccaata tggcatcgtc
     2101 gcgacgcagg cgcagtaagt ccttgtgcag gcgatagatc ggcgcatgcc gggtccgctc
     2161 ggacaaatcc agacaggacc gctgaaatgt cgcttcatcg gccgggtccg ggacggcagc
     2221 ttgggcctcg ggcgttgcat actccgggaa ctcggcgagg aatttcttgc ggccttcgta
     2281 cacttttgcg gccagatccg agtggtgatc ggcaaaaaac aaaaacggat tcgaagcgcc
     2341 gaactcctgc cccatgaaca acatcggcgt ttcaggcgca aggagcagca gcgccgcgat
     2401 tgcccgatac cgggcatggg atgccagtgc gtgaatgcga tctccatgca gatgattggc
     2461 gacctgatca tgattttgca gataaaacac gaatccgggc gccggctcgt tcttgacgac
     2521 cgtgccgcgc ggtttcttct gccattcata ccgctggccc tgatagacga accccctttt
     2581 gatgacggac agcagctcct gggcggtgcc gcgataatcc gaatagtacg cttggccgcg
     2641 accggtcagg gcgacacggc tgatatgatg gaagtcatcg ctccagactc cgtcgaggcc
     2701 ccagccgcca tcctcgatcg actgaatcgt cgtgatcaac tgcgtttcgc attccgcgat
     2761 caacacaatc gagcgtgctc ctgccgcgac ccgcgcctga cgcgagagct cagcgagcac
     2821 atgcaccggg ctgtcatcgt agatggcatg gacggcatcc agtcgtaatc catcgagctt
     2881 gaactcggtg atccaatagc acgcgttctg gataaaaaac tcccggaccg gccctgatcc
     2941 gtgtccgtca aaattaatcg cttggcccca ttcgttggga tgccgatcag taaggtagag
     3001 atcgctgtac gcaggcaggt aattcccgtc tggtccaaag tggttgtaga cgacgtcgag
     3061 gatgacgccg agtccccgtt cgtgcgcggc atccacgaac cgcttgagcg catccgggtc
     3121 gccatacacg tgcgccggtg caaacaggtc cacaccgtcg tacccccaat tccatcgacc
     3181 ggggaattcg gcgagcggca tcaattcgat caaggtgaca ccgcaatctt tcagttcatc
     3241 caattgtgcg atcgtggcgt cgagcgttcc ttccggcgtg aaggtaccca cgtgcagctc
     3301 gtagatgact tgaccctgca tccgaacacc ttgccattct gggtgacgcc acgtgtaggt
     3361 ggacggatca acgatcagcg atggaccgtg gggtccctcg ggttgatagc gggaacaggg
     3421 atccggatag gcttcgccgt tatccagccg gtagcggtag cgcatgccgc cggttgcgat
     3481 cgggacgagg ccggaaaagt accccgcctc gtctttcatt aatgggaaac tccgtcctcc
     3541 ctcttccaga acgacatcca cccgcgcccg cttgggcgcc catacacgga atcggacgcc
     3601 ccccgtttcc acctgggcgc ccagcgtcat gtgccatgac gtctgacttg ccgtggtggg
     3661 ggttctaccc gagatggtcg agcttgttgg cataggtcct cgtcatggcg aagggtagcc
     3721 gcggaatgcg acattcctta ccgtttcaca atattcatat ttatccgatt gttcagtttg
     3781 tagcattctg gaacgggaag gacagcctgg gaaggaccct agtatgggac ttaccgctga
     3841 taattgaaca cgaccgctca aaagacaagg cgagtgaacc ggctgactgc ccaagatgga
     3901 gagcgctgac cctcccgcac atcagtgaga gaaaatgagg cgcctatggg tcttcctagg
     3961 cgcaggtctc cccgcaacga cgtattcata tttataggta ggcaattggc attctcgcgg
     4021 tgaggaggac atatgaggaa gcagaattgg cacaccatat tgataggcgg cgtgtgtatg
     4081 atcgcctctg gttgcattac cgatgcaacg acggaactca ccaaagcgcc cttcgacgcg
     4141 acatcgcagc tgacaaacgg cacgaccgcg gccttatccg agttcacgga cccgttgtcg
     4201 gattttacct cgagcacgac gcccggcgcg gaggggggga tcgttccttc acgagcgaag
     4261 aaacggttgc agatcttcac ggcgtcttcc tatgaaaatc tccgcgccga tatttcgcgc
     4321 ggtcatggtg agtacctggt gtcgctcgcc aaactcgccg gcgtgccgtc aggtcaattt
     4381 aggcagttcc aaaccttcat gcaggattct tacagcacga tgttcgagaa ggaaattccg
     4441 attcccgagt cggcccacca gatcgtggag atcgcctggg cagccggata tggcagcagc
     4501 gttacccgtt aatgcgtcag acagtttctt gcggcggctg cacacccgcg cctctgctcg
     4561 ttagagaggg gggacgtcag gagggtttca gcgtttccgg aacggccggt tcaatactgg
     4621 tcagtcctat ctcctggaac agcctctgcc aggcagccga cgacaattcg tgtttcatca
     4681 gcaccgctcg agcgtgccca agcgccgtgg tctgttcagg cgccaacaca tgggaggtga
     4741 cgatgactac gggcagtgac gcggtttcgg gattcgcctt cagctgtgcc aacacctcaa
     4801 agccgtcgat cttcggcatg ttaagatcca acagaatcaa cgctggcttt aattgccgcg
     4861 caagcttcag cccgtcctcc cctcctgtgg cctcgacaat ggaacatttg aaatcttcga
     4921 gatagtgtcg gagaatgtac cggtcggctt cctggtcgtc aatgacgagc accagcggcg
     4981 gacctgccga cggttcccgt actatgccgg tgacacgatg caggcggtcc agcaaccaga
     5041 ttcgccgcag cggcttgaca cagtaatcat ccgctccaag cgccatcgcc ttccgcggat
     5101 cctctaccga ggacacaatc agcaccggga gcttcttcgt cgcctcctga cttttgagct
     5161 ctgcaagcca ttgccaggct gactcatcgg gtaagaggac atccagcacg atggcggcga
     5221 ataactggcg gcgcagttgt tcgcgcgcct ggcgtatgct gcctgctgcg atcggccgga
     5281 atgcggtatt acgaagatat ttgtcgtaca agagacgggt ctccggctca tcttcgacaa
     5341 ccaagattgg cagccggaag tcgtctggcc gtacttgcac cggttcgatc tgctccgcca
     5401 gttcaatcgt gttgacggaa tagtggatgg gaatcgtggc cacgaaggtg gagccggcac
     5461 ctattcggct cgtcacggtg atggaaccgc ccaagatatt ggccagtttg cgacacagag
     5521 gcaatcccag accggttcct ttcacctgta gctgcaacgg atgatccact tgtgagaatt
     5581 cttcgaagat gcggttctgg tcttccgatg caatgccgat tcccgtatcc gccaccgaaa
     5641 aggctacggc gcgtccgtca tcggccagcg ccgccgtcac gcgcacttca cctcgcggcg
     5701 taaatttgat cgcattcgac acaaaattac gcaggatttg ggacaccttg ccctcgtcgg
     5761 tgttcatgag cggaattccg gagggttctt cgaatactaa attcaccgct tcagtgacca
     5821 gcaacggtcg tagcatgccc cgcaacgcgc tgaatagagt ggccacctcg aattcagaga
     5881 ctttgacggt gatctttccg gcttcaatct tggcgaggtc caagaggtca ttgaccagct
     5941 caagcagatc ttcgccgctc ttccggatgt agccgacttg tttctcctgc tcgctgttta
     6001 actctccgtc cgtacgctcg agcagcaagt gggacaaggc cagaattgca ttgacgggcg
     6061 tcctgaactc atggctcata ttcgatagaa agcgtgattt catttcgtcg gcccgccgca
     6121 agtggcccgc cttttcatcc agctctgcat aaagggccac cacgccccgg ttggtgtctt
     6181 ccaactcgcc gttgagcctg accagctcct gctgccgttc atgcagctct tcgagcgccc
     6241 gcagcaattc ctgattttgc tgctgcactt cctccatcgg gttttgcggc tcctgcttgc
     6301 cgagttgctt gatgacccgc tccaacgctt tccccacgaa cagcggagcg cggtgcggga
     6361 gaattttcac caagcggatg agactccctt ccggcgagtc cgtccggact tcaccatgat
     6421 ccatcaagcg tctcgcggag gtcaccgcag actcccactc tggatcgagg tttcggccgt
     6481 tcggcggacc ggacttcgcg gatccgccgc gggaccaccc gaggttcaca agcagcagtt
     6541 gcggcacggt cgtaccttcg atcagaaatt caatcagggc cggtcgtttg gaatgcacga
     6601 acaatcgagc aatttccgac acggccgtcg agatacgggt ttggtcctgt tcctcaaacg
     6661 tcagaaggcg cgacacctgc cgtgcccgtt gccttgcggt gactacgtca tcttcgtagt
     6721 tgatctcgac tacggtgatc gtgttggaca tgattactcc gaagacggaa ctccggccga
     6781 ggggtgttgt ctgatcacaa ccacggtggc gtcgtcccgg ccgcgcttga agtcccggta
     6841 cagaatgccg gcgatgaggc tcggatctcg tgacagaagc ccgggatacc ggtcgagatc
     6901 ccaatgcgtc gcgatcccgt cgctgtgtag cagcaacagc gactgcgcat gccatggctg
     6961 cgtaaactct gagaccttgc gatagtcatg gccaatgatg ccgttgtgcg atacgagatg
     7021 gcgccgctcc gtcccgttca caaccaaggc agcgatattg ccgatgcccg cgaagcgcaa
     7081 gagacccttg gatccatcca agacggcgat gccgactgca gcgccgcgcg tgggtttcgc
     7141 ggcctgatgg gccacttcca ctaactcgac tggtgtccgg ttggccggag cccgacgtac
     7201 ggcttctact atggcagttg cggcctgtga cgcgatcgac ccgtgaccca agccatcggc
     7261 tacggcgcac agccaaccgt ccgcagacca gcggacaatc caattgtcgc cgcacaccgc
     7321 ttctccaggc ttggcttgtc ggacgactcc gacggcttgc gcttgagact cctctcgccc
     7381 accctggcgg ctcctcactc ttgccacaag ggcggttcct ttgcccggct gcgagtagac
     7441 gtcaaactgc tctgcagttc gaagaacagc gcccaatccg gtaccgggac tgccggcagt
     7501 cgaatagccg tcttctagag aacggctgac attcgacatg cccggcccct tatcaagtga
     7561 cagcacttcg attccctcgt atccctcggg accgaggcac cgtatcagca gttcaccgcc
     7621 ttgtgcatgt ttgacgaggt tagttgcgag ttcggtcacg acaagagcga catggccgat
     7681 cgtcggctca tcgatccccg cctcctgggc gagtgaaacg gcggctcgcc gcgccgcggc
     7741 cggttgactc tgttccgtca ccggaacaca cacttgccgg tctgtcgcca agccgatcac
     7801 ttccacctca caatgcggac cgtcgtgcct ttgccaggct tcgattcgat cgtaaactcg
     7861 ttgacgagtc gcttcgagcc gctcaatccc aatcccaatc ccccgcgcgt cgtatagccg
     7921 tcggtcatgg ccagctcaac atcggcaatg ccggggccct ggtctgtaaa tgtcacttgt
     7981 acaccgcggc gtgtcccctc atgcacggcc tgcaggcaca tctcgccgcc tccgccgtgc
     8041 accaatgtgt tcctggcgag ttcgcttgcc gctgtcacga gcttggtttg atcgaccagc
     8101 gacaggccct gcaagacggc gcaatcgcgc accaactgtc gggccttgat gatatcgtcc
     8161 gtcgaccgca gggcgatcaa ttcatcgcgt aagacggcca ttgcccgaca tctttccagc
     8221 cgacttcagc agcgccatgc cggcatcgac attgagggca gtcttgatgc tcggcagcgc
     8281 cagacccaat tcaaccagtg taatcgccac cgcaggccgc atgcccacca cgacggtctc
     8341 ggcatcgagc acgcgcgaca tggacgcaat gttgccgatc atgcggccga taaacgaatc
     8401 gacgacgtcc acggaggaaa tgtccaagag cactccacgc gcgcgctcgg ccacgatccg
     8461 ctcggtcagc tcgtcctgca gcgtcatcgc cacgcgatcg tgcatgtcga cttgaatcgt
     8521 caccaacaga tagggaccca tcttcaatat gggaatgcgt tccatctagt actcttcctc
     8581 aattctgcgc acgttgtact ccgccacctg ccctatcccg atgtggcggc ggcgttccgg
     8641 gtcacggtgg ctcccaggcg ctggagtgcg atcatgaatg catcggccag cgtggctttg
     8701 gtgacaatat cgtttaaatt cacacccagg tgcacgatgg tctgggcaat ctgcggacgg
     8761 atgccgctga taatgcaatc ggcgcccatc aaccgggtcg ccgcgaccgt tttcatcaaa
     8821 tgctgggcga ccagagtatc gaccgttggg acgccggtga tgtcgatgat cgcaatgcgt
     8881 gcgcccgtct cgactacctg ctgcaacagg ttttccatga cgacctgggt ccgcgcgctg
     8941 tcgagcgtcc caatgagcgg cagagccagt acttcgtccc acaactggac gaccggagtg
     9001 gataactcca ttaattcctg ttgttgcctc atgatgacgg actcccgagt ctcctgatag
     9061 gcctcggtcg tgtacagacc cagcttgtcc aacaaggcag tcagatccca aagtgtttca
     9121 gccagcttct tggtgtcctg accggcccgt tgttgagcca gatcaaacag gggttgcttg
     9181 agtgagaaga cgaaggtcgc ggtctcggag gggctgaagc cctgttcggc acgggtgcgg
     9241 gtaatttcgg cgagatgctc cttgaccggc ttccagctcg gtgaaaaggg gtccgagctc
     9301 actgagaacg cttccgagaa caaatccaaa aatcgccgtg acatttcccg caggtctgac
     9361 tccttcacga gatcgcgtct cagcgtcaga gcggaaagtt gatattccat ccagtgcttc
     9421 aggatgagat cctgcctgtt tcgaatttcg tcagccatct ggctattctg cgacatcggc
     9481 gtcctcccct ttttgatgat gatccgccac acagtctcac tgttcgtcgt tggcttccgc
     9541 accggagcga gacacgctcc gcgcacgccg agtgccagac tcagcggacc tcggcgacgc
     9601 cttctcggct gctattggca ggctgtgcgt cgaggacctc ccgtatggtg cggtacagtt
     9661 cgtcgacggc ggcttccttc gtcagcaaga cggctccccc ggcattgacg atcgccctcc
     9721 gagcttcttc acccgcctgc acggagagtc cgatgacgac cgtctcggga aagcgggctt
     9781 tgatgcgtgc cgtcgcctca atgccattct ttttcggcat gttgatgtcc atcacgacca
     9841 gcgcgggacg aagctgctcg acgagctcaa tcgcttcctc gccgttagat ccttctccca
     9901 ctatcacgac atcctcgtag gattccagca ggctccgcaa gccctggcgt accatcgtgt
     9961 ggtcgtcaac cagcaacaac cggatcgtgt tgcccggagg cggatgaaag ggggcatggg
    10021 gaatacggta aatcgattgc tcggattcct gcttgggtct tgctgccgta gcggcaactt
    10081 cagatgtctt gccggcgtgg tcggccgtct tcgccttcac aaggggcagc atcatcgtca
    10141 cggtcgtgcc ctgctccggc atgctcttga cctcgaggcg tccgcccatc gcctgcatgc
    10201 gttcccgcac gctgaacagc ccgaaatgct ctttctctga tttggcctcc atcgcggccg
    10261 gatcgaagcc acggccgctg tcctgcacct cgatgaacag ttgttgctcc tgatccacct
    10321 tcaatgacac cgttgcacgg tcggtcccgg catgtttggc aacattgatc agcaattcac
    10381 gcaccgattg aaacagcaag accgattgat cgtccggcaa gggcacctgc tcggttgaga
    10441 ggcggacttc gacgagcaga ccatgcaaag gcatctgctt ggccaaccag gtgagagccg
    10501 agggcaatcc caactcatcc agcaccggtg gattcagctt ggccatgagt gatcgtgtgt
    10561 actcgccggc tttttccagc aggttgtcga cttccgccac cagctgctgg gcagccggcg
    10621 cctgccctga gagcttctga cgaagctgcg tgaccttgat tcgtcccaat gccagcatct
    10681 gcgccaggta gtcgtggagc tctgaggcca gccgtttccg ttcgcgttgc tccgccaggg
    10741 tgagttctgt ggccagtgcc tgaagccgct gattggacgc cacgagatcc cgtgtacgct
    10801 ccgtgacacg ctgctcgagg tcgccgttca gccggcgcag cgcttcttcg gcgcgtttgc
    10861 gttgacctaa ttccatttcc aatgcatggt tggccacctt caattgcgcg gtcctggagg
    10921 caatccgaag ctccaaatca gcattcaacg tcacgatggc attccgggct gcggtatgct
    10981 cctcccgtaa tctccgctgc tccagcgcgt gccgaaccgc ctcccccaac cgtcccagcc
    11041 ggtccttgag cagatagtcg gccgcgccgg ctcgcatcag cgccaccgca tcctcatccc
    11101 cgatggcatg ggagacgatg atgaacggaa tcgcctggtc gcgttgacgg gtgagttcca
    11161 gcgcctcgga cgaactgaaa tgcggcaggt tgtggtcgta aatgatcacg tcaggagtgc
    11221 tctcatcgag tgcgatgatg aacgcatccc gtgtatcgac gcggcgcgat tccggctcga
    11281 atccgctctt tttcagctca cggacgacca attctgcatc ggtcgcatca tcttccacga
    11341 tcaacacgcg caacgttgcc atagcccaca ctccttccga ggtcaatttg attgtgacgc
    11401 ggattcgagc gccaattccg acggaaactg attgagcaat acccaataca acccgacctg
    11461 cctgacagca tcactaaatt gtggaaagtc gactggcttg acgacgtaac tgttcactcc
    11521 aaggttgtag cttttgacga gatcctgctc ttcacgcgaa gaggtcagga cgactatggg
    11581 aatccccttg gtccgtggat ccgacttgca tcgttgtaag acttccagcc catccacttt
    11641 gggtagcttg aggtcaagga ggatgagttt ggtgttccga catggactgc gctcggcgta
    11701 acggccggtg ccgaacagac attccagcgc ctcttcaccg tctcgcacga cgtgtatttt
    11761 gttggcaata tgatgtttgt gaaaggcccg taatgtgatt tcgacgtctt ccggattatc
    11821 ttcgatcagg agaatctctg tcccatttgt catggcaatg cctttcgtaa tgagaaaaag
    11881 aaggtggcgc ccccgtcgac cttaccttcg gcccacacgc gtcctccgtg ccgttgcacg
    11941 atgcgctgca cgatggccag gcccactcca gtgccttcaa actcctcctt aaggtgcaat
    12001 cgttgaaaca ctccaaacag tttgtgcacg tatttcatgt cgaagccgac gccgttgtct
    12061 cggacaaaat acaccaaggc atcagcctga cggtcgtcgg gcagccaacc gacctcaatc
    12121 tgagccaccg ggcgatggcg agtaaattta aaggcattgg ccaggagatt cgtccagacg
    12181 agcttcaaca gccgccagtc tccatgacag ggcggcagat caccgatcgt cagatcaacg
    12241 gtgcgaccgt cgtccgtcac tttgagttcg cccgcgactt cctgtgcgat ctcgaccatc
    12301 ttcgtcggac ggaagtccat gccgatgcgg cccaagcggg agaattccag caagtcgtcg
    12361 atcagttcac cggcttggcc ggcagccttc ctgacaatcc caaggcagcg ccgcgcctcg
    12421 gagttcaagt gcgcgccatg gtcttcttct aaaatgcgga tgtacgcgtc gatcgtgcga
    12481 agcggcgcac gaagatcgtg cgatacagag taggagaagc tttgcagttc cttattcgcg
    12541 tcggcgagac ccgatttctg ttgattcaat tcgtcattcg ccatcgtcag agcccggtca
    12601 cgatctctga gcgcttgctc ggcccttttt tcctccgtga cgtcacggaa gacgacgacg
    12661 aggccggcga tttcctcgcc cgcacgaatc ggcatcgcac tgtacaccac gtcgaagcac
    12721 gtgccgtcct tgcgcacaaa gacatccggg taattgatca gcggcgtgcc cttctggagc
    12781 acctgaaacc ccgcgcagtc gtgtgcggga aagggtgtcc catcacgatg cttgtaatgg
    12841 gtcacatcat gcatcttcag cccgagcaat tcctccttgc tccagccgaa cagtttttca
    12901 gcggcgtgat ttatggaaat gacgagtcca tgccgattga cggtgtacag gccttcaccc
    12961 atattgttga tgaccgcttc ctgataggcc aaggcgtgcc gtagccgttc gcggctttct
    13021 ttcaatgccc ggtcctgctc gacccgttgg gtgatgtccc gggcaatctt cgatgcgccg
    13081 atcacctttc cgtgggtatc gaacagcggc gaaatcgtca gcgaaacgtg aacctcgctt
    13141 ccgtctttgc ggcgccggac cgtttcgtag ggttcgaccg tccggccgcg cgaaatctcc
    13201 tcgagaatgc ggcgttcttc atcttgtcta tcggctggaa tgaccacggt cacaggccgg
    13261 ccaatcatct cgtcggctcg atagccaaac agtcgttccg ccgcccggtt ccaactcgtg
    13321 accacgcctt gtagattctt actcacaatg gcatcgtccg acgatgcgac aatggccgct
    13381 aaatgcgccg tcgcccgctc ggctcgttga cggtcagaga tatccctgag ataggctgtg
    13441 aaaaacggtg gccgacccgg ccgccgagtc acggcaatag aaaactcgac cgggatttct
    13501 gttccgtcgg cccggagcgc gggcatctga atccgtcgtc ctatcacgga cgcctcgcct
    13561 gtctccaggt accgtcgtaa cccgacttga tgactctccc gcaaacgcgg cggaatgatc
    13621 aggtccgcga ccgaacggcc cagggcgtgc gcctgagtat acccaaacgt tttttcagca
    13681 gccggattga aatcctggat ccgtccgtcg gcatccatcg taatcaaggc gtcgagggaa
    13741 gattcaaaga tcgcacgcgt ccgctcttcg ctttccctga ggtgatcatc gatgcgcttt
    13801 tgctctgtca cgtcaatgag catattgacc gcgccgatga cggtgcccga ggcatcacag
    13861 atggggtctg gatgaggcac cacgtgccgc ttggacccgt caggccgttc gatgacgatt
    13921 tcctctccgc gaacggcccg tccttctcgc agggtgaccg ccatcggaca ttcgtccaac
    13981 ggcaatggag ttccgtcagg ccgatagatc ttccatgacc cgcaccagag atcctttccg
    14041 atttccggct cacgccccca taagctcacc gccgcctggt tgtagagctg aattcgccct
    14101 tgggcgtcgc aggtatacac ggccgccggg agactttgaa tgagttcgcg gtaccgactc
    14161 tggctcagcc gcaaggcctc ctgatcgtgc tttcgctcag tgatgtcaaa attcacaccg
    14221 gtaaggcgca ccggttccct cgcgcgatct ctaagccacc gccagcgccc tgccaaccac
    14281 cgaatctctc cgttgggttt gacgatgcga aattctcgat cttccgggtc gccgctcctt
    14341 cgcgatcgtt cgacggcctc taccatctcg tggcggtcat caggatggag acaagactcc
    14401 cacgccgcct gggtcttccc gaaatcgtcc ggtcgaagcc cgtacatggc atagagttca
    14461 gggctccagc tgttcacacc tgtgtcaaga ttccagtcga acgaacctac ctgagacgct
    14521 tgttgggcca atgtcagccg ttcttccgtt acacgcaggc cctgggccag ttcctcggcc
    14581 cgtcgccggg cccgatgcat cacctgtccg accgcgatga gtgtcagccc gacgactcca
    14641 tacaggacaa gtccaacttg ttgtgacagg tccgtcatgt cgagtgagaa acgcggtggg
    14701 acaaaaaacc agactgcagc gaacccgccc agtaacgtcg cagtcaacga ggcagacact
    14761 cccgcatacc aagttgtgaa cgcaatggac acgaagaacg tggcataagg aagatgatcg
    14821 ccaagatagg gatcaaggag aagacggcag ccgagtgcca gaatcgtcgc gaacagtgcg
    14881 acaatatgtt ccctgattac ggcggtcaat ccgcttggtt tcatttgccc accaaattcc
    14941 ggcgtgcagg tcacaccact cgcctcccgg tgaagcacca ggcttcgtaa gttattgggg
    15001 aagcggagga agtatagggg attttagact agacacaccc ctagggggac ccctagtttt
    15061 cggtggatgt cagccaaagc aaaagaagtg aaccggagtc ggtccactgt agaggaattt
    15121 ctaccgctca gcagggtggc cgatacacgt ccatcatttc aatattggtg gtggcaggat
    15181 aaaactcccc gaaccgattc atgacccatc ctttatgggc gtgcacctcc ttgagaagta
    15241 tgggttgagc gacataggtc gggcagactg gagagatcac gacgaattga tgagacgcgc
    15301 tttcgcgttc gctccaatag cagtcaaaga cctggacctg caccacctga tcaccaagca
    15361 tggtttggtc cggaggctcc gcaccacagg tcatgtttcc attcaatacc agcatgagaa
    15421 acagggcaat ggcgttcata tacatctcct ctttgaggct cactgcaccg cgaatcaatt
    15481 ctccgaagcc tcatagagcc cttctgtatc acgtcgtttg gaggctcgcc cctccatggt
    15541 gcaagaagag ttccgacatg cagttgagca atacgcctgt ggggagtggc gaaagaactg
    15601 attaacagcc cataccgggg accacgatag cggcgcctcc atacagaccg ggacaaactg
    15661 tccgccgtga tgcgcaccta tccttctcgc tagcatttag tgactagcct taaatgccta
    15721 gtgagaacgt gacgtgaaaa agaccgacgc gtgtgactcg ggtgaatcct aaccgctttc
    15781 aggggttctc cctgtgaggc aagtcggtcg aatcggtcac gatcagttgt gacgaggaaa
    15841 gcgcttccca acgacccttc acccataacc cgacgacaaa gtcggtccca tgcgattgag
    15901 tttggctctc gtcggcccga cctccgatcc atttcattga ccggtctgtt catccttgcc
    15961 ctcgtgtggt tcctgcatca agctcaggac tttgcagttc cccttacgct ggccctgctc
    16021 ctgcactttt tgttcatgcc gatcgtgcgc tggttcaaac gtcatcacgt cgtggaaccg
    16081 gtggccgccg caatcatcct cctggccatg acgggactgg tgaccattac gatgtatgaa
    16141 ttggccgcgc cagcggcgca gtggctcgaa aaagcaccac gcgcgttccg tgtaatcgaa
    16201 agcaagctgc tcccggtcaa ggaatcggtg tctcaactct ccgaagccac ggaggccatg
    16261 aaccgtgtcg gacagaccga ttcatctccc gccgtccgca aggtggaagt ggtgccggac
    16321 cggcgagcgg cagtggtgag cctcttggga aaaaccacgg ccgggatggg cacgaccctt
    16381 cttctcttgt acttcttatt ggcgtccgga gatctgtttg ccaaaaaact cctcaccgtc
    16441 ttgtcccggc gcgaggatcg tggctgtgcc gccgacattc tcgaaggcct cgagcgacaa
    16501 atgtcccaat atttattcca aactgtcgtg attcagagcg gatttgggtt tatcttgtgg
    16561 ttgtctctct ggacactcgg gatgccgaat cctggtcttt gggcggccgc agccgccatt
    16621 ttgcaaatcg tgccgtacat tggtggtctc gcggtgctgg tcctcattgc cgtggtggca
    16681 gccgtgacgt ttgacaacac gtggtctatc gccggg
//
LOCUS       DASTQY010000371         3937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1731037, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000371 DASTQY010000000
VERSION     DASTQY010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731037"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="VFR82_12130"
     CDS             complement(<1..1185)
                     /locus_tag="VFR82_12130"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU4502389.1"
                     /translation="MARQEHTRQSNAPYDGSALIADPIHKYVSFTVPSSHADPHEQTE
                     KDLIDSPWVQRLRHIYQLQSARWVYPSAEHTRFVHSIGTMHVAGRFARHLYPFLKKST
                     PDVPSANYVEEFLRVTALVHDIGHGPFCHFFDDNYLHALHASHEKLGQLIIRDHLGPI
                     IRKIRRSPSGPFAKGEELHPDHIAHVILKEKGKDNSRLPRWLNMLQPVISGSYTADNL
                     DYVLRDSYMCGVAVGPVDLTRLIHYTIVTEKGFTIHKTGLPALQMFLNTRMYLYSNVY
                     FHRTTRAIDIHLRDIFGDTMKLLFPHDPRKKMDEYLTLTDSSLLEQVRGWKTSRHSGE
                     RRLGEEWARILGRDVKWKMAYSTVLKEKGQERGMDFPSHSHFEKQIRKELPKHLHHVA
                     FRV"
     gene            complement(1201..1680)
                     /locus_tag="VFR82_12135"
     CDS             complement(1201..1680)
                     /locus_tag="VFR82_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEU4502390.1"
                     /translation="MASFRSLLRQVLHLEESPQRTALAFAVGAAIAFCPLYGFHMVLV
                     GICSWAFRLNFLALLAGAFINNPWTLVPVLGATYWIGAVILGRSDRPSFDWNELGFRA
                     IYEQVMPYAVPFAVGGIVLSLVAAIVSYPLALYLITKHRQQASARQVEPLPPADPLR"
     gene            complement(1696..2769)
                     /gene="thiL"
                     /locus_tag="VFR82_12140"
     CDS             complement(1696..2769)
                     /gene="thiL"
                     /locus_tag="VFR82_12140"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEU4502391.1"
                     /translation="MPLISPGSMVARPRRKAIATLNEFDLIRALQRRYRSDGPGVLRG
                     IGDDAAVIASNPRRSHLFTTDLLAEGIHFDLATATFVDVGFRAATANLSDIAAMGGTP
                     EYILVSLAMPQSASKVQVEQLYRGLMTACRPHGVRLIGGDISASAAGWFLNITLVGSC
                     PARDVLLRSGSKPGDNLYVTGTLGDSRAGLQLLQNGPRRSRSFISSRHRRLLVARHLR
                     PTARLREGCWLARGRWATSAIDISDGLSGDLRHLCDESQVGVDLDLSMLPITPACRAY
                     ALGLKQDPAMLALAGGEDYELLFTVPSHRSARFERATRAHRIRVTRVGRMLPSGRGLH
                     MIMPSGRRQPLPLVSYEHFRSQR"
     gene            complement(2747..>3937)
                     /locus_tag="VFR82_12145"
     CDS             complement(2747..>3937)
                     /locus_tag="VFR82_12145"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEU4502392.1"
                     /translation="DKVGMDFRGDPSAALLEVLDPEQNSTFTDHYLGVPFDLSEVMFI
                     TTANLIDPILPALRDRMEIIEIPGYTEEEKLGIAQRYLIPRQLNEHGITEKHVKIAEA
                     AIRQIIMHYTREAGVRNLEREIANVMRKVAKKVAEGKGLGFPVNAGNLHKYLGVPKFV
                     PESELEKDEIGVATGLAWTESGGDVLYIEATVMKGKGVLTLTGHLGDVMKESAQAALS
                     YVRSREKSLGINPDMFTKQDLHIHVPAGAIPKDGPSAGITMATAIASALAQVPARRDL
                     AMTGEITLRGRVLPIGGLKEKILAAKRAKLTTVILPKRNKKDLDEIPKHILKGIQLVF
                     ADTMDDVMKVALRRRQPAKKKTGHPQAPQEPTIPTTRIDKSRRAERTAHARAARLHAS
                     HLPR"
BASE COUNT          797 a         1069 c         1225 g          846 t
ORIGIN      
        1 gacacggaac gcgacgtgat gcaggtgctt gggcagttcc tttcgaatct gcttttcgaa
       61 gtgcgaatgg ctcgggaagt ccattccccg ttcttgaccc ttttctttca ataccgtgct
      121 gtacgccatt ttccacttca cgtcgcggcc caaaatacgt gcccattcct cgccgagacg
      181 ccgttcgccg ctgtgacggg atgtcttcca cccgcgtact tgttcgagca aggaggaatc
      241 ggtcagcgtt agatattcat ccatcttctt gcgcggatcg tgggggaaca gcagcttcat
      301 tgtgtccccg aaaatgtcgc gaagatggat gtcgatggct ctggtcgtcc ggtgaaagta
      361 tacattcgag tagaggtaca ttctggtgtt caagaacatc tgcagcgccg gaagtcccgt
      421 tttgtgaatc gtaaatccct tctccgtgac gatcgtgtaa tgaatgagcc tggtcagatc
      481 gaccggaccg acagcgactc cgcacatata ggagtcacgg aggacgtaat ccagattgtc
      541 ggccgtgtaa cttccagaaa tcacgggctg tagcatgttc agccagcggg ggagacgtga
      601 gttatccttg cccttttcct tcaaaataac atgggcgatg tgatcggggt ggagttcttc
      661 gcccttggca aacggcccgg atggactgcg gcgaatcttt ctgatgatcg ggcccagatg
      721 atcccgaatg atcaactgcc ccagtttctc atggctcgcg tgaagggcgt gcaagtagtt
      781 gtcgtcgaag aaatggcaga aggggccgtg gccgatgtcg tggacaagcg cggtgacacg
      841 aagaaattcc tcgacgtagt tggccgaagg aacatcgggc gtgctttttt tcaggaacgg
      901 atagagatgg cgtgcaaacc ggcccgccac gtgcatggtg ccgatggaat ggacgaagcg
      961 ggtatgctcc gcagagggat atacccagcg agcgctctgg agctgataaa tatgacggag
     1021 gcgttgcacc cagggggaat cgatgagatc tttttcggtt tgttcgtgcg gatcggcatg
     1081 gctcgatggc acggtgaagg atacatactt gtgaatcgga tcagcgatca gggccgagcc
     1141 atcgtaggga gcgttggatt ggcgtgtgtg ctcctgtcgt gccatgggcg cgacagcatc
     1201 ttatcgtagg gggtcggcgg gcggcaatgg ctccacctgg cgcgctgatg cctgttgtcg
     1261 atgcttggta atcagataca aggcgagagg gtatgacaca atagccgcca ccagactgag
     1321 cacgatgcca cccacggcga agggcacggc atagggcatg acctgttcat agatggcgcg
     1381 gaagcccagt tcattccagt cgaacgaagg ccggtcggag cgtccgagaa tgacggcgcc
     1441 gatccaatag gtggcgccga ggacgggaac cagcgtccag ggattgttga tgaacgcccc
     1501 tgcaagcagc gccagaaaat tcagtctgaa agcccagcta caaatgccta ccaggaccat
     1561 gtgaaagccg tacaagggac agaacgcaat cgcggctccc actgcaaagg cgagcgccgt
     1621 gcgttggggc gattcctcga gatggagcac ctgtcttaac agcgatcgaa aagaggccat
     1681 gagactgggt ttacattagc gttgagaccg gaaatgctca taactgacta acggcaacgg
     1741 ctgccggcga ccgcttggca tgatcatgtg gagacctcgg ccgctgggca gcattcttcc
     1801 gactcgcgtg acgcggatac ggtgtgccct cgtcgcccgt tcgaaacgag cggatcggtg
     1861 cgacggaacg gtaaacagca gttcatagtc ttctccgccg gccaacgcca gcatcgccgg
     1921 atcctgcttg aggccgagag cataggcgcg gcaggcggga gttatcggca gcatcgacag
     1981 atcgagatcg acgccgactt ggctttcatc gcagaggtgt cggagatcgc cggagagccc
     2041 gtcggagata tcgatggccg atgtcgccca acgccctctc gccagccagc acccttctcg
     2101 aaggcgcgca gtcgggcgca ggtgtctggc caccaacagc cgtcgatggc gggacgaaat
     2161 gaaggagcgg ctccttcgag ggccgttttg taagagttgc agtcccgccc gggagtcccc
     2221 caacgttccg gtcacgtaca ggttgtcgcc gggcttggaa ccgcttcgga gcaggacatc
     2281 gcgtgccggg catgacccga ccagcgtgat gttgaggaac cagcctgccg ccgatgcaga
     2341 gatgtcgccg ccaatcagtc tgacaccatg gggtcggcag gcggtcatga gaccacgata
     2401 cagctgttcg acctgcactt tggaggcgga ttgaggcatg gcgagagaga ccaggatgta
     2461 ttcaggggtt ccgcccatgg ccgcaatatc gcttaaattg gccgtcgccg cacgaaaccc
     2521 tacgtcgaca aaggttgcgg tcgcaaggtc aaaatggatg ccttccgcca gcagatcggt
     2581 cgtgaacaag tggctgcgac gagggtttga ggcgatgacg gcggcatcat cgccgatgcc
     2641 ccgaagcact ccagggccat cgcttcgata ccgtcgctgc agtgctcgaa tgaggtcaaa
     2701 ttcgttgagt gtggcaatgg ccttgcgccg gggccgagcc accatactac cgggggagat
     2761 gagaggcatg gaggcgagct gctcgtgcgt gggctgtccg ctcggcccgc ctcgatttgt
     2821 caatgcgcgt ggtcggtatc gtgggttcct gcggcgcttg agggtgaccg gtcttctttt
     2881 tggctggctg tcggcgccgc aaggccactt tcatgacgtc gtccatagta tcggcgaata
     2941 cgagttgaat gcctttgaga atgtgttttg ggatttcgtc gagatctttc ttgttgcgct
     3001 taggcagaat gactgtggtg agcttggccc gctttgcggc aagaattttt tccttcaagc
     3061 cgccgatcgg caggacccgc ccgcgcaagg tgatttcccc ggtcatggcg agatcccggc
     3121 gtgccggcac ctgtgccaat gccgatgcga tggcagtggc catcgtgatg ccggccgacg
     3181 gtccgtcttt gggaatggcg ccggccggaa catggatgtg caagtcctgc ttggtaaaca
     3241 tgtcggggtt gatccccaga gatttttcac gcgatcgcac atagctcaag gcggcctgtg
     3301 ccgattcttt catgacatcg cccaggtgtc cggtcagagt caacacgcct ttgcccttca
     3361 tgacggtggc ctcgatatag aggacgtcgc cgcccgactc tgtccaggcc aatcccgtcg
     3421 cgacaccgat ctcgtccttt tcaagttcgg attccgggac gaacttgggg acgcccagat
     3481 acttgtgaag atttccggca ttgaccggaa agcccagtcc tttgccctcg gccactttct
     3541 tcgccacttt ccgcatgaca ttggcaattt cccgctcgag attccggacg cccgcttctc
     3601 gcgtgtaatg catgataatt tggcggatcg ccgcctcggc aatcttgacg tgcttttcgg
     3661 taatgccatg ctcgttgagt tgccgaggga tcaggtagcg ctgcgcgatg ccaagctttt
     3721 cttcctcggt gtagccggga atctcgatga tctccatgcg gtcccgcaag gcgggtagaa
     3781 tcgggtcaat caagttcgcg gtggtgatga acatcacttc cgagaggtcg aatggaacgc
     3841 ccaggtaatg gtcggtgaag gtgctgtttt gttctgggtc gagcacctcg agaagggcgg
     3901 ccgaaggatc gccccggaag tccatcccca ctttgtc
//
LOCUS       DASTQY010000372         5268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1398000, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000372 DASTQY010000000
VERSION     DASTQY010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398000"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VFR82_12150"
     CDS             complement(<1..485)
                     /locus_tag="VFR82_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HEU4502393.1"
                     /translation="MADTKGRPSRLRSLRDSVKHITKRLSEGDADVIHRNDEQAREIE
                     KLRVQIQSMEEEIRRLYQSRYQLDQANKQNEKLVNTLQEAKAQIEALRAEVEKLTAPP
                     STYAIFSSLNQDGTGNVYVSGRKMKVSLHPSLMGKGLRKGQEVILNEALNVIEARGFD
                     V"
     gene            complement(624..1256)
                     /gene="thiE"
                     /locus_tag="VFR82_12155"
     CDS             complement(624..1256)
                     /gene="thiE"
                     /locus_tag="VFR82_12155"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU4502394.1"
                     /translation="MPSLESRLLLVTDRQQTKGRPLMSVLAQALKAGKISIQLRERDL
                     CAKDLLTLAGEIQQLMEPHKGQLVINDRLDVALSLDEAGVHLRSNSLPVGVARRLLSP
                     LRVLGASAHSVAEAVEVEANGADYVVFGPVYETPSKRLFGLPLGLPTLEEAARAVRVP
                     IFAIGGVTADRAREVRRAGAFGVAVITAILCADDVEVATTALLEAVTASV"
     gene            complement(1343..2116)
                     /locus_tag="VFR82_12160"
     CDS             complement(1343..2116)
                     /locus_tag="VFR82_12160"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEU4502395.1"
                     /translation="MHNDRLVIAGREFKSRLWVGTGKYKDFAETKKAIEVSGADVVTV
                     AVRRVNITDRSKENLLDFIDPKKYTILPNTAGCYTVEDAVRYARLARAAGVSDLVKLE
                     VLGDEKTLFPDTAGLIEAAKILIKEGFIVLPYTNDDPIVAKKLVDIGCPAVMPLAAPI
                     GSGLGIRNPYNLKIIMETVKVPIVVDAGVGTASDAALAMEYGADAVLMNTAIAGAQDP
                     IAMAEAMKYAVHAGRLAYKAGRIPRKLYATASSPIEGML"
     gene            complement(2106..2363)
                     /gene="thiS"
                     /locus_tag="VFR82_12165"
     CDS             complement(2106..2363)
                     /gene="thiS"
                     /locus_tag="VFR82_12165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEU4502396.1"
                     /translation="MAEPLQIQVNGECRDCRIGATVGDLLRELDIKTERVAVELNLEI
                     LDRQEFDRRGLKAGDRVEILSFIGGGGPVEGWIRRVPTHAQ"
     gene            2473..2946
                     /locus_tag="VFR82_12170"
     CDS             2473..2946
                     /locus_tag="VFR82_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEU4502397.1"
                     /translation="MAIPQHNVQLAGFFRSIAELLAAQRANPYRVRAYRRGADALLAL
                     DEDIAEVAQRQALEEIEGIGRDLSEKIQEFLRTGKISLLETLRMPLPEHIREWAGLPG
                     LSESLVAYLHSRLNIGTLADLEQLVRSRMLRTVPGFSGSDEDLLVAIEDLRRRTL"
     gene            complement(2947..4320)
                     /locus_tag="VFR82_12175"
     CDS             complement(2947..4320)
                     /locus_tag="VFR82_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU4502398.1"
                     /translation="MMEHNQRRRLWMLGPMVVVALLSGVLIGKGWERTGHASETYEEL
                     RTFSEVLTQVQKNYVDDTKVKDLVQGAIRGMLSTLDPHSAYMTPEMYKEMQVETKGEF
                     GGVGIQIGVKENRLAVIAPIDGTPAQRAGIKAGDYITKVNEESTKDLSLMDAVQKMRG
                     PKGTKVNLTIQRDGTSDPLQFTLVRDTIKIESVKSKVMDNIGYVKLTQFQEATGRDLG
                     RVLKQFKDQKVQSTILDLRNNPGGLLTAAVEVSEQFLPNGKLVVYTKGRESKKDEWFA
                     KGRDQMDDSPMIILVNEGSASASEIVAGALQDYGRAVIVGTTSFGKGSVQTILPLGDG
                     SGLRLTTAKYYTPKGRSIQSTGITPDIVIKPQPATTVAKADTKPSDKEPDNKTSKSPI
                     TGGPGKEQSAATPKDDAVQRNGATPGAGAAPLDLSGEPSLDQDVQLQKAVELLKTWKI
                     FKELRPS"
     gene            complement(4354..>5268)
                     /locus_tag="VFR82_12180"
     CDS             complement(4354..>5268)
                     /locus_tag="VFR82_12180"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU4502399.1"
                     /translation="RKKDQEIETINAQIARLREGVQVRQQAILARLRVQYMEGRFGHW
                     KPLLASDSYGDFQRRLRYLSAVSERDYSLIETFKSDMATMQEAERQREAARVGMLAYK
                     RSTEKHLEEINAVKKEKKLYLTKLTQEKESYERALQELERSASRIDSLLRELEQRRRA
                     AAAKPPAGHLQRGVKGGLPWPAEGSVLTYFGRQKHPTFNTYVQRKGIEIKTLEGSAIH
                     AVMPGTVVYADWLKGYGLVIILDHANGFFSLYAHASKILTSVGAQVASGQAIGETGDT
                     GMTGENTLYFELREGAEAVDPLQWLARR"
BASE COUNT         1004 a         1611 c         1411 g         1242 t
ORIGIN      
        1 tggacatcga acccgcgagc ctcgatgacg ttcaaggctt cattcaaaat gacttcctga
       61 cccttccgca agcccttgcc cataagggac gggtgcaagc tcaccttcat tttgcgtccc
      121 gacacgtaca cgttacccgt cccatcctgg ttgagactgg aaaaaatggc gtatgtcgaa
      181 ggcggagcag tcagtttttc gacttcagca cgcagcgctt cgatttgcgc cttggcttcc
      241 tggagggtat tgaccagttt ttcgttttgc ttgttggctt gatcgagttg gtaacgggat
      301 tggtacagac gacggatttc ttcttccatc gactgaatct gtacgcgaag cttttcgatc
      361 tctctcgctt gttcgtcgtt acgatgaatc acgtctgcat ctccctcgga caaccgcttg
      421 gtgatgtgct tcacggaatc tcggagggac cgtaggcggc ttggtcttcc tttcgtatca
      481 gccatggaac cacctgggac aagcgacaag ccctgctaaa tcgcatagtc caagtcttga
      541 gcggattcta acactcgtta tttttacggt caactagacg acagattccg gcgcgcagaa
      601 tgcgccgcta cgacacggca cgatcacacc gacgcagtca ccgcctcgag tagcgcggtg
      661 gtcgcgactt cgacatcgtc tgcacaaagg atggccgtga tcacggcgac gccaaatgcg
      721 cctgcacggc gcacctctcg tgcgcgatcg gcagtcacgc cgccgattgc aaagatcggc
      781 acccgaacgg ctcgcgcagc ctcttccaac gtcggcaacc cgagcggaag cccgaacagt
      841 cgtttcgacg gtgtctcata caccggaccg aacacgacgt agtccgcgcc gttcgcttcg
      901 acttccacgg cttccgccac tgaatgcgcc gacgcaccga gcacccggag tgggctgagc
      961 aacctgcggg cgacgcccac tggcaggctg ttgctgcgta gatggacccc ggcttcgtcg
     1021 agcgacagtg ccacatcgag acggtcgttg atcacgagct ggcctttatg aggctccata
     1081 agttgctgta tctcacctgc aagagtgagg agatctttcg cgcagagatc gcgctctcgc
     1141 aattggatgg aaatcttccc ggccttcaac gcttgggcaa ggaccgacat cagcggcctg
     1201 cccttggttt gttgacgatc cgtgacgagg aggagacggg attcaagcga cggcatctag
     1261 ggacgtgatc aggcttggga tagagaatgt acacttcggc atgtgatcac tggtgtgagc
     1321 gaaaaggctc ggcattgtgc tcctagagca tgccttcaat gggactgctt gccgtcgcat
     1381 acagtttccg gggaatgcgg cccgctttgt aggccaaccg accggcgtgc acggcgtatt
     1441 tcatggcttc tgccatagca ataggatctt gtgcaccggc gatggccgtg ttcatgagta
     1501 cggcatcggc tccatactcc atcgccagcg ccgcatctga agccgtgccg actcccgcat
     1561 caacgacgat ggggacctta actgtttcca tgatgatttt cagattatac ggattgcgta
     1621 tgcccaggcc cgatccgatg ggtgccgcca gtggcatgac ggcaggacag ccgatgtcca
     1681 cgagcttctt ggcgacgatg ggatcgtcgt tcgtgtaagg aaggacaatg aatccttcct
     1741 tgatgagaat cttggctgct tcgatcaagc ctgctgtatc ggggaacaat gttttctcat
     1801 cccccagcac ttcaagcttg acgagatcag acactccggc cgcccgtgcg agccgcgcat
     1861 accgcacggc atcctccacc gtgtagcacc cggccgtatt gggaaggatc gtatatttct
     1921 tcggatcgat gaagtcgagc agattctctt tcgagcgatc cgtgatgttc acgcggcgca
     1981 ccgccaccgt cacgacgtcc gctccggaga cttcgatcgc cttcttggtc tcggcaaaat
     2041 ccttgtactt gccggttccc acccagagcc gagacttaaa ctcacggccg gcgatcacca
     2101 gtcggtcatt gtgcatgggt cgggaccctc cttatccagc cctcaactgg accaccgccg
     2161 ccgatgaagc tcaaaatctc cacccgatcc cctgctttga ggccgcgccg atcgaattcc
     2221 tgacgatcca agatttccag gtttaattcc acagccaccc gctcggtctt gatgtccaat
     2281 tcgcgcaaca gatcaccgac cgtggctccg attcggcaat cgcggcattc gccgttgacc
     2341 tggatctgca atggctcagc catgaactat catcctaggg aaccctaatt cccgttgtca
     2401 atcaacgcgc acccgcttcc tagttgcctc tctcgcgctg aaccctgcag aatagaacgc
     2461 cggaacaacc cgatggccat cccacagcac aacgtgcagc ttgccggctt ctttcgttcc
     2521 atcgccgagc ttcttgccgc tcagcgggcg aatccctatc gagtgcgggc ctaccgacgc
     2581 ggagccgacg ccttgttggc attggatgaa gacatcgcag aagtggcgca acggcaagcg
     2641 ctggaagaga tcgagggcat cggcagggac ttatcggaga aaatccagga atttctcagg
     2701 accgggaaga tctcactcct ggaaactctc agaatgccct tgcccgagca tattagagaa
     2761 tgggcaggct taccgggtct gtccgaatcg ctggtcgcct acctgcacag ccgtctgaat
     2821 atcggcactc tggctgactt ggaacaactc gtacgatcgc gcatgctcag gacggttccg
     2881 ggattttccg gttccgatga ggacctgctc gtggccatcg aagacctccg gcgccggacg
     2941 ctctgatcag gacggccgga gctctttaaa tatcttccag gttttgagca gttccacggc
     3001 cttctgcaac tgcacatcct gatccagcga gggctcgccg gacagatcaa gaggagcagc
     3061 tccagctcca ggcgtcgccc cgtttctttg cacggcgtcg tctttcggag tggcggcgga
     3121 ctgctctttt cccgggccgc ccgtaatggg cgatttcgac gtcttgttgt ccggttcttt
     3181 atcactcggt ttggtgtcgg ctttcgccac cgtggttgca ggctgcggct tgatgacaat
     3241 gtctggcgtg atgccggtcg attggatcga ccgccctttc ggcgtgtaat acttcgctgt
     3301 tgtaagccgc aatcccgatc cgtcaccgag cggcaaaatc gtctggacgg atcccttgcc
     3361 aaatgaggtc gtgccgacga tcacggctcg tccatagtct tgtaacgcgc cggccacaat
     3421 ttcggacgcg ctggccgatc cttcattgac gaggataatc atcggtgagt cgtccatttg
     3481 atccctgccc tttgcgaacc attcgtcttt tttgctctct cggcctttcg tataaactac
     3541 cagctttccg ttcggcagga actgctccga cacctccacc gccgccgtca acagcccgcc
     3601 gggattgttt cgtaagtcga ggatcgtgga ctgaaccttc tggtccttga actgcttcag
     3661 cacccgaccc aggtcacggc cggttgcttc ttgaaattgc gtgagtttca cgtatccgat
     3721 gttgtccatg accttggatt tcacgctttc aatcttgatg gtatcccgca caagagtgaa
     3781 ctgtagggga tcagacgtgc cgtctcgctg aatggtcaga ttcactttcg ttcctttggg
     3841 tccacgcatt ttttgcacgg catccatgag cgaaagatct ttcgtggact cctcattgac
     3901 cttggtgatg tagtcaccgg ctttgatccc ggcccgctgc gccggagtgc catcgatcgg
     3961 ggcaatcaca gccaaccggt tctccttgac gccgatctga ataccgaccc ctccgaactc
     4021 gcccttggtc tcaacttgca tctccttata catttctggc gtcatgtagg ccgaatgcgg
     4081 atcgagcgtc gacagcatgc cccgaatagc tccttgcaca aggtccttca ccttggtgtc
     4141 atcgacatag tttttttgga cctgcgttaa cacttccgag aaagttctca gctcctcata
     4201 cgtttcggaa gcatgcccgg tccgttccca tcccttgccg attaacacgc cggacaggag
     4261 ggccaccacg accatcgggc ccaacatcca aagtcgccgt cgttggttgt gttccatcat
     4321 ttgcgtcctt tcgcctgcct ggtcatcttt cgcttaccgt ctcgcaagcc attgaagagg
     4381 gtcgactgct tcggcccctt cgcgtagttc gaagtataag gtattttcgc cggtcattcc
     4441 cgtgtcaccg gtctccccga tcgcctgccc tgacgccacc tgcgccccta cggacgtcaa
     4501 aattttcgac gcgtgggcat agagggaaaa gaagccgttg gcatgatcga ggattataac
     4561 cagtccgtag cctttcaacc agtccgcata gacgaccgtc ccgggcatca cggcatggat
     4621 ggcgctccct tccaatgtct tgatctcgat gcccttgcgc tgcacgtagg tgttgaatgt
     4681 gggatgcttc tggcggccga agtaggtcag cacgctgcct tcggcgggcc acggcaagcc
     4741 tcctttcacg ccgcgctgca ggtgtcctgc gggaggcttg gcggccgcgg ctcgacgccg
     4801 ctgttccagc tcgcggagaa ggctgtcgat ccgtgaggcg gatcgctcga gctcctgcaa
     4861 ggcccgctcg taggactcct tttcctgcgt cagcttggtc aggtacagct ttttttcctt
     4921 cttgacggcg ttgatctcct ccagatgctt ttctgtgctg cgcttgtagg ccagcatgcc
     4981 gacgcgcgcc gcctcccgct gccgttcggc ttcctgcatg gtggccatgt cactcttaaa
     5041 ggtctcgatc agactgtagt cccgctccga aaccgccgac agatagcgca ggcgccgttg
     5101 gaaatccccg tacgaatcgg acgcgagcaa cggtttccaa tgtccgaacc gcccctccat
     5161 atactgcaca cggagcctgg ccaagatcgc ctgttgcctg acctgcacac cttcgcgcaa
     5221 gcgtgcaatc tgggcgttga tcgtctcgat ctcttgatct ttttttcg
//
LOCUS       DASTQY010000373         4470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1172256, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000373 DASTQY010000000
VERSION     DASTQY010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172256"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFR82_12185"
     CDS             complement(<1..533)
                     /locus_tag="VFR82_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502400.1"
                     /translation="MRRRLRSVCTCLLMLLLLAVHAGCEYVRPTLNAPLEKWEPGGGY
                     RFTNVAPPGGNNSDSFLFIAAFSGGGTRASSLAFGALRELANQQIVWEGKQKRLLDEL
                     DLIHALSGGTFTGGYYALYRDQIFHDFEYRFLRKDWETELRHRVFRSPSNWVRLWSPY
                     FGRAHLMAELLNEALFD"
     gene            complement(623..2434)
                     /locus_tag="VFR82_12190"
     CDS             complement(623..2434)
                     /locus_tag="VFR82_12190"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEU4502401.1"
                     /translation="MKNLTRPLYAALIGATALMFGTIWLTPLGAETESGQPSLKEIIE
                     KRSKGLSESERTAVGVPDDSLGRGTPRSSVRGFLLAARERNYVQAAEYLDLRNLPPGM
                     TESQGPELARQLRIVLDRTLWIDLELLSTSPEGEQNDNLPVARERVGRISAEGKAYDL
                     FLQRVPRGDGVYIWKFADVTVADIPELYQQVGYGQLERIFPAWMFDVSILGINLWLWA
                     AVIALAVVLYPAAMLVTKLMAAALRPLNAEFAEQFERLFRGPVTLLIWTVMVRSAGTF
                     LGSSIALGALSQARTVQMIGLAWLLMRVVDFMAQRAGANLERKGLGGSRVLLMPVARL
                     IKFVALAGAVLLWLDNAGYKVTTLLAGLSISGVAVALASQKSLENLFGAVTLFTTRPV
                     KVGDFCGFGDKVGFVEEIGLRATRIRTLERSIITVANAEFANLHLDNYSARDRFWYHP
                     TLQLRYETTPDQIRYILVEVRKMLYSHPKVLPEPLHVRFKSFGQYSLDVEVFAYIGVL
                     DYGESLEVAEDLNLRMMDIVTAAGSDFAFPSEVQYSLPGKPLDEERAKAVSQQVKEWK
                     AKQELYLPNFPADKIAAIKNSLEYPVQGSPQFSPARV"
     gene            complement(2583..3452)
                     /locus_tag="VFR82_12195"
     CDS             complement(2583..3452)
                     /locus_tag="VFR82_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502402.1"
                     /translation="MSHSVLRRTALALLIISTVVLTLRPSVHAEEKADTPFHDKLMIR
                     GGWAYVFGATANVSVGGPVLGVGANVDFTRTLGGDTSTDAFRLDGLYRFNDRHAIGLS
                     WYRVGLGGEKILNQQVQIRDQIVAAGASTQTSLSFNIYRLLYNYSFYRNDKVELGFSP
                     GLYIAQTKFNFAGQGTITPPGGVPTPGSTTVVNEQVTLPLPSVGFLANYNITPKLQFQ
                     SRFDFFYLAVNEYAGSMFEIYLGLEYRLHQHFAMGAAYDRLTTELSGSGDEGFNVNFG
                     YNLAYVYATLYLF"
     gene            complement(3672..>4470)
                     /locus_tag="VFR82_12200"
     CDS             complement(3672..>4470)
                     /locus_tag="VFR82_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU4502403.1"
                     /translation="VLATLDDPRFSAEHAKNSLWKPYDFIFDVGPGVYFLEPYNASKI
                     PVLFVHGINGTPANFRFLIEHLDRTKYQPWLYYYPSGVHLSGLADHLDQTMKKLQLHH
                     GFTQYHVIAHSMGGLVSRGFLLRNQSGQSRARVALYITISTPWAGHKAAQAGVEYAPA
                     VVYVWNDMVPQSAYLTDLFFSNHNGSSVHRPLPSSIQHHLLFGYKHSGTAIGECSDST
                     VTLASQLYPGAQEDAIMLHGFDETHMSILDSVETSRLINRLVDEATP"
BASE COUNT         1028 a         1248 c         1230 g          964 t
ORIGIN      
        1 ttatcgaaca gtgcttcatt gaggagttcg gccatgaggt gcgcccggcc gaaatagggc
       61 gaccacaacc ggacccaatt actgggcgac cgaaagaccc ggtgtctgag ttcggtctcc
      121 caatccttcc gaaggaaacg gtattcaaag tcatgaaaga tttggtcgcg atacaacgcg
      181 taatagccgc cggtaaacgt gcctccggat agagcatgaa tcagatcgag ttcatcgagc
      241 agacgcttct gttttccttc ccatacaatc tgctggttgg cgagttctcg aagcgcgcca
      301 aacgcgaggc ttgatgcgcg ggtacctccg cctgaaaagg cagcaataaa gagaaaactg
      361 tccgagttat ttccccctgg cggggcaaca ttggtgaaac gatatccgcc accaggctcc
      421 catttttcca agggagcgtt gagggtcgga cgcacatact cgcatcccgc gtggacggcg
      481 agcaggagga gcatcagcag gcacgtgcac acgcttctca aacgtcttcg catgttgtcc
      541 tttccgatgg cgcgaacagc gttgtaccag aaaccgggcg ctttctcatc tcaactggcc
      601 tgtcgcaggt ccgccgactc cgtcacaccc gggccggtga aaattgtggc gagccctgga
      661 caggatactc caacgagttt ttgattgcgg caatcttgtc ggcaggaaag tttggcaggt
      721 agagttcctg cttcgctttc cactccttga cttgctggct cacggccttg gcacgctcct
      781 catcgagcgg tttccctggc aatgagtact gcacctcgga tggaaacgca aaatccgatc
      841 cggcagcggt cacaatatcc atcattctca gattgagatc ttcggccact tccaacgact
      901 cgccataatc cagcacgcca atgtaggcga acacctcaac gtccagggag tattgaccaa
      961 agctcttgaa gcgaacatgg agcggctccg gcaacacttt cgggtgggag tagagcattt
     1021 tgcgaacttc cacgagaatg tatcggattt gatcgggggt ggtctcatac cgcaattgga
     1081 gggtcggatg ataccagaag cggtcccgtg ctgagtaatt gtcaagatga aggttggcga
     1141 actcggcatt ggcaaccgtg atgatagacc gttccagcgt tcgaatccgt gtggcccgca
     1201 gtccgatttc ttccacgaag ccaaccttat cgccaaaacc acagaaatcc ccgactttca
     1261 ccggtctggt tgtaaacagc gtcactgcgc cgaaaagatt ttcgaggctt ttttgtgacg
     1321 ccagcgccac ggcgacgccg ctgatgctga ggccggccag cagtgtcgtg accttgtacc
     1381 cggcgttatc cagccacagc agcaccgcac cggcgagggc gacaaacttg atcaggcggg
     1441 caacaggcat caacagcacc cgagatcctc cgagaccttt tcgttcgagg tttgcaccag
     1501 cgcgctgagc cataaaatcg accacgcgca tcaataacca ggccaggccg atcatctgaa
     1561 cagtccgagc ttgactcagc gccccgaggg caattgacga tccaaggaag gtaccggcgc
     1621 ttctcaccat gacggtccag atcaacaacg tgaccggtcc cctgaagagc cgctcaaact
     1681 gctccgcgaa ctcagcgttg agaggacgga gagcggcggc catcagcttt gtcacaagca
     1741 tggcagcggg gtagagaaca actgccagcg cgatcacggc agcccataac caaagattga
     1801 tgccgaggat cgagacgtcg aacatccacg ccgggaaaat ccgttcgagc tggccatatc
     1861 ccacttgttg atatagctca ggaatgtccg cgacggtcac atcggcaaat ttccagatgt
     1921 acacaccgtc gcctcgcgga acacgttgca ggaagaggtc ataggccttg ccttcggccg
     1981 aaatgcgtcc gacccgctct cgcgcaacgg gcagattgtc gttctgttct ccctcaggac
     2041 tggtgctcag caattccagg tcgatccaga gtgttcggtc gagcacgatc cgcaactgtc
     2101 tggccagttc gggaccctga ctttcagtca tacctggcgg caggttacgg agatccagat
     2161 attcggcggc ttgaacgtag ttgcgttctc tcgccgcgag gaggaagccc ctgacgctgg
     2221 atcggggcgt cccacgaccg agcgaatcat ccggaacacc aaccgccgtc cgttccgatt
     2281 cgctcagacc tttggaccgc ttctcgatga tttccttgag gctgggctgt ccggactccg
     2341 tttcggcacc cagcggtgtc aaccaaatcg tcccgaacat gagtgccgtc gctccaatga
     2401 gagccgcata cagaggtcga gtcaggtttt tcatatccag ttcctccttc cactagtccc
     2461 aatatggact ccggaatttt gaggcagccg ttatacgaat tcttttctcg ggattcgtat
     2521 ttgaacttct cagattgctg tcttgaggac agttattcaa tgaagaatca acgattggcc
     2581 ggtcaaaaaa ggtagagggt ggcgtaaacg taggccaggt tgtagccgaa gttgacgttg
     2641 aacccttcgt ccccgcttcc actcaactcg gtagtcagac ggtcataggc agcacccatg
     2701 gcgaaatgtt gatgcagccg atattcgagt ccgagataga tctcgaacat cgatcccgcg
     2761 tattcgttca cggccaaata gaaaaagtca aaacggcttt ggaattgcaa ttttggcgtg
     2821 atgttgtagt tcgcaagaaa gccgaccgac ggaagcggaa gtgtgacctg ttcgttcaca
     2881 accgtggtac tgccgggtgt aggaacgccc cccggtggtg taatggttcc ttgacccgcg
     2941 aagttgaact tcgtttgtgc gatatacagc cccggagaga atccgagctc cactttatcg
     3001 ttgcgataaa acgaatagtt gtagagcaat cggtagatat tgaagctcaa gctggtctga
     3061 gtggacgcac cggctgcgac gatttggtcc cgaatctgaa cttgttggtt gagaatcttc
     3121 tctcccccca agccgacccg ataccatgac agccctatgg catgccggtc gttgaaccgg
     3181 tacaagccgt ctaaccggaa tgcatcggtg ctcgtatccc cgccaagggt tcgggtgaag
     3241 tccacgtttg cgccgacccc taagacagga ccgccgactg atacgttggc ggttgccccg
     3301 aagacatagg cccagccgcc acggatcatt aacttatcgt gaaagggcgt atcagccttt
     3361 tcctcggcat gaacagaggg tctgagagtc agcaccacgg ttgagatgat aagcagcgcc
     3421 agcgccgttc tgcgtagcac cgaatgactc atgacactac ctccgtgccg aatcgagggt
     3481 tcaaagtcgc gtagtctagc gaaaattgct taagctgtct atgcaatcgg cggggaacga
     3541 ggccattctc tatcccccct ctgttgcatg ccgccctgtt gctctcacag ctccttggcg
     3601 gactcatcct tgcctgcaag ttgcaagaac cgtttgactt ctcctcgcgg gaaatatccc
     3661 aatgccgtgc ttcaaggcgt tgcctcatcg actagacgat tgatcaagcg ggaggtttct
     3721 acgctgtcca agatactcat gtgcgtctcg tcgaacccat gcaacataat ggcgtcctcc
     3781 tgggcgcctg gatagagttg actcgccagc gtgaccgtgc tgtcagaaca ctcgccgatc
     3841 gcagtcccgg aatgcttgta accaaagagc agatggtgct ggatgctcga aggcaacggt
     3901 cgatgcacag acgagccgtt gtgattgctg aagaagagat cggtgaggta ggcgctttgg
     3961 gggaccatgt cgttccagac atagaccacc gcgggggcat actcgacgcc ggcttgcgca
     4021 gctttgtgtc ccgcccatgg cgtggagatc gtgatataga gtgccacgcg ggcgcggctc
     4081 tgtccggact ggtttcgcaa cagaaacccg cgggacacca gaccacccat gctatgagca
     4141 atcacatggt attgagtgaa gccgtggtgc aactgcaatt tcttcatcgt ctggtcaaga
     4201 tggtcggcaa ggccggagag atggacgcct gaaggatagt agtacaacca cggttgatac
     4261 ttcgttcggt caagatgttc gatgagaaag cgaaagttgg ccggcgtgcc gttaatcccg
     4321 tgcacgaaga gcaccggaat cttgctcgca ttgtacggct cgagaaagta cacgccggga
     4381 ccgacatcga aaatgaagtc atacggtttc cacaggctgt tcttcgcatg ttcagcgctg
     4441 aaacgtggat cgtcgagcgt ggcaagcacc
//
LOCUS       DASTQY010000374         4818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_361292, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000374 DASTQY010000000
VERSION     DASTQY010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361292"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VFR82_12205"
     CDS             complement(<1..630)
                     /locus_tag="VFR82_12205"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEU4502404.1"
                     /translation="MAALHAKNVMALTGAARGLPQAVPNSACFHCDVCCRFPDPDSVL
                     RPYFTDEEIASAVDRGLKASAFPDLNGSQVTLVPNPQGEGFLCPAFESTTGRCLIYDQ
                     RPLDCRLYPLMLMWNAGHDEVVLGWDSTCPYTKEHVPESIRRHADQVMAVLEEQSTVQ
                     RIADHPRLVGRFQSEALVLAPLPGLTRAISARWGSEQCRRLVLEDLARLK"
     gene            747..1760
                     /locus_tag="VFR82_12210"
     CDS             747..1760
                     /locus_tag="VFR82_12210"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU4502405.1"
                     /translation="MALSPEGPQYDPQVLLNSQPVIITVIDPVTHKVVFQNQTSQNKF
                     GDISNLTCHEKIASCATPCAFCKMPEAVDTGKPSASEVPLPNDEYLLVQWAQVKTASG
                     KTHIVETITDITATKRQQAEAEGLVKRLSNTNRDLMQANQLLRDQSIRDSLTGLYNHS
                     HFQETLVHLCALALRSMRPLSLLFIDLDNFKQINDVHGHTMGDQVLREVGWLLDSQQA
                     PGRGTARSSDFSARYGGEEFALILPDTSMDGALSAAERLRHRVTTLTMLPELTGLNTH
                     PFALTCSIGVATFPIHASIPSDLVVAADAAVYAAKTAGKNCVRLASPANPTAPLFAPL
                     VGL"
     gene            1913..3301
                     /locus_tag="VFR82_12215"
     CDS             1913..3301
                     /locus_tag="VFR82_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502406.1"
                     /translation="MTVPDEDSVSASWRIGLEPLPLTREQIAFFEALGPQLLSFYKAL
                     NRLYTESAKGTTQPRWIAAWLDQGKPEALIKYSQMKRFRDELPAVIRPDVIPTQDGMV
                     ITELDSVPGGIGLTACMSRLYANQPGFSSGSDKAGMQLVGGPDGMLNGFAGMLRSHRG
                     QQEGVVAIVVSDEAKDYRPEMGWLSKELTVRGLETYRIEPREIRFTEGKLLLGTEGGD
                     RPIGLIYRFYELFDLPNIPKNELIQYAAKKSWVTITPPYKPALEEKAAFGLLHHPTLV
                     AYWRRELGEDVFLHLSTVMPRTWVLDPAPVPPAAVIPGLSVGGRAVTNWSELAEATQK
                     ERHLVIKPSGFSELAWGSRGVSVGHDLPQSEWTAAIESALRSFPHTAYVLQEFHKGRQ
                     YHLEYFDPVTAEIQSMAGRARLSPYYFVTGEKVDLAGILATVCPADKKVIHGMKDAIM
                     VPCAAVQGKATE"
     gene            3298..3552
                     /locus_tag="VFR82_12220"
     CDS             3298..3552
                     /locus_tag="VFR82_12220"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU4502407.1"
                     /translation="MTMTLYHVQWCPECDVVRRKLAELNLPYEEVIVPDFRPQRKEVF
                     AVSGQYYVPVLQDGETVLSDTGEILAHLESKAESRRLANG"
     gene            3654..>4818
                     /locus_tag="VFR82_12225"
     CDS             3654..>4818
                     /locus_tag="VFR82_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEU4502408.1"
                     /translation="MEDWRRILADSIVKPKDLAERFGMDEQEIEAIVGPYPMRITPTV
                     LGTIKEKGDPIWKQVMPDPAELTDIDAEDDPLEEDVMSPVPHLVHRYPDRVLLMVTNQ
                     CPIYCRFCTRKRLVGKPGFLKKGELDQAIAYLQEHTEVRDVILSGGDPLLLPDHLLER
                     ILKALRSIPHLEVIRLGSRVPGTLPERITPKLCSIVKKYHPVYMNLHFNHPDELTPAV
                     KEACGMLADAGIPLGAQTVLLKGVNDDPETMKRLVHQLLLARVKPYYLYQADLTKGTN
                     HFRTTIESGLNIIKALQGHTSGMAVPHFVIDAPGGGGKIPLLPDDYLVHMDEDGAVLR
                     NYENKTFHYPQPKAGNGRELPMVGARTSYDDSSCADSYPERDGYSSWGNSCGGE"
BASE COUNT         1052 a         1413 c         1350 g         1003 t
ORIGIN      
        1 cttgaggcgt gcgagatcct caagaaccag ccgtcggcac tgttccgatc cccatcgcgc
       61 agagatcgcc cgggtaaggc ccggaagagg agcgagcacc agcgcctccg attggaaccg
      121 ccccaccagc ctcggatggt ccgcgatccg ctggaccgtg gactgctctt cgagaaccgc
      181 catgacctgg tcggcatgtc ggcggatgga ctcaggcacg tgctccttgg tgtaaggaca
      241 ggtgctgtcc caccccagca ctacctcgtc atggccggcg ttccacatga gcattagcgg
      301 atacagtcgg caatcgagcg gccgctggtc atagatgagg cacctgccgg tcgtggactc
      361 aaaggcgggg cagagaaatc cttctccctg aggattcggc accaatgtga cctgagagcc
      421 gttcaggtcg ggaaatgcgc tggccttcag accacgatcg acagcgctgg cgatttcctc
      481 atctgtgaaa tagggccgca gcacgctgtc cggatcggga aaccggcagc agacgtcgca
      541 atggaagcag gcgctattcg gtaccgcttg aggcaagccg cgggccgcac ctgtaagagc
      601 catcacattc ttggcgtgca gtgccgccat agagctttca ccgaaagagc acattctatc
      661 accattcgta aaacactcaa attcttgacc ggaaccgccc tgactgaaat actgattcca
      721 gcattgttcc tccaacgtac acaacgatgg cgctctcccc agaagggcct caatatgatc
      781 cccaagtgtt gctcaactcg caaccggtga tcattaccgt cattgaccct gtcactcaca
      841 aagtcgtatt tcaaaaccag acctcccaga acaagttcgg cgatatttcg aatctgacct
      901 gccatgaaaa gattgctagt tgcgccacgc cctgtgcctt ctgcaagatg cctgaagcgg
      961 ttgataccgg aaagcctagc gccagcgagg tgccgcttcc caatgacgag tacctgctcg
     1021 ttcagtgggc gcaggtcaag acggcgagcg gaaagactca tatcgtcgaa acgatcactg
     1081 atatcacggc gaccaagcgc cagcaggcgg aagctgaagg cctggtcaag agactgtcaa
     1141 acacgaatcg agacctcatg caagccaatc agctcttgcg cgaccaatcc attcgcgaca
     1201 gtttgacggg actgtacaac cattcgcact ttcaagaaac cttggtccac ctctgcgccc
     1261 tggcacttcg ttcaatgcgt ccgctctcgc tgctcttcat cgatcttgat aatttcaaac
     1321 agatcaacga tgtgcacggc catacgatgg gcgatcaagt cctgagggaa gtcggctggt
     1381 tattggatag ccaacaggcg cccggacgag gcacggcccg gtcgagcgat ttctcagcgc
     1441 gctacggcgg agaagagttc gccctcattc ttccggacac ctcaatggac ggggcgctca
     1501 gtgccgccga acgcctccgc caccgcgtta ccacgctcac aatgcttccg gagctgactg
     1561 gcctcaacac acatccgttt gccctgacgt gcagcatcgg cgtcgccacg tttccgattc
     1621 atgcgtccat tccatccgat ctcgtcgtgg ccgccgatgc cgcggtctac gcggcgaaga
     1681 cggccggtaa gaactgcgtg cgattggctt ctcccgcgaa tcccactgca ccgctattcg
     1741 ctccccttgt aggtttgtaa agtcgcgtga tacgattctc gcatctgatt ccatgtcgtg
     1801 aacggattcc ctgtctgatt tggcaacctc cgaccttctt taccgctgct ccagtatccg
     1861 tgatgccctg attgaagcag gtcttttcgc gcctcaaccc gcagcctctt ccgtgacggt
     1921 tcctgacgaa gactcggttt ctgcctcttg gcgcatcggc cttgaacctt tgccactcac
     1981 ccgtgagcag attgcgtttt ttgaggcgct cggaccgcaa ctcctgtctt tttacaaggc
     2041 cttgaaccgc ctctatacgg aaagcgcgaa agggacgacg cagccgcgct ggattgcggc
     2101 ctggctcgat caggggaaac ccgaggcgct gatcaaatat agccagatga aacgcttccg
     2161 cgatgagctg ccggcggtca tacggccgga tgtcattccc acacaggatg gaatggtgat
     2221 cacggagctt gattccgtcc ctggtggcat cggactcacc gcctgtatgt cgaggcttta
     2281 tgcgaaccag cccggctttt catccgggag cgacaaggct ggcatgcaac tcgtcggtgg
     2341 ccctgacgga atgctcaatg gattcgcggg aatgctccgc tcccatcggg gacagcagga
     2401 gggcgtcgtc gccatcgtcg tgtctgacga ggcgaaggat taccggcccg aaatgggctg
     2461 gctctccaag gaactgacgg ttcgggggct tgaaacttat cgcatcgaac cgcgcgagat
     2521 tcgctttacc gaagggaaac tattgctggg gacggaaggc ggcgaccgtc cgatcggcct
     2581 tatctatcga ttctatgaac tcttcgacct gccgaacatt cctaaaaatg agttgattca
     2641 atacgcggcc aaaaaaagtt gggtgactat tacgcctcca tacaagccgg cactggagga
     2701 aaaagcggcc tttgggctct tacaccatcc cacgttggtt gcctactggc ggagagagtt
     2761 aggggaggat gtgtttttgc atctctcgac ggtcatgcct cgcacgtggg tgctggatcc
     2821 ggctcctgtg ccgcctgctg ccgtgattcc cggcctgtcg gtgggcggcc gcgcagtcac
     2881 gaactggagc gagttggccg aagccacaca aaaagagcgg catcttgtca tcaagccgtc
     2941 cggattttcg gagctagcct ggggtagtcg aggagtctcg gtcggccatg atctccccca
     3001 gagcgaatgg acagctgcca tcgagtcagc tctccgctcc ttcccacata cggcgtatgt
     3061 cttgcaagaa tttcacaagg gccggcagta tcacttggaa tattttgatc cggtaacggc
     3121 agaaatccaa tctatggccg gccgcgcgag gctgtcgccg tactatttcg tcactggcga
     3181 aaaagtcgac ttggctggta ttctggccac ggtctgtccc gcggataaaa aagtgatcca
     3241 tggcatgaag gatgcgatca tggtcccctg cgcggcagtt caagggaagg cgaccgaatg
     3301 acgatgacac tgtatcatgt gcagtggtgc cccgagtgtg acgtggtgcg gcggaaacta
     3361 gccgaattaa acctgcccta tgaggaggtg atcgttccgg atttccgacc gcagcgtaaa
     3421 gaagtctttg ccgtatcggg ccagtattat gtaccggttc tacaagacgg cgagaccgta
     3481 ttgagcgaca caggcgaaat tctggcccac ttggagagca aggcggaatc gcgtcgtctg
     3541 gctaatggct aatcgaagtc ccctggtatc ccaccacttc ccagggacat gagcaacagc
     3601 cgtcaaggtg tgcgcctgcc gctattccag cgtctgctta taaggagtat tgaatggagg
     3661 actggagacg cattctcgcc gacagcatcg tcaagccgaa ggacttggcc gagcgcttcg
     3721 ggatggatga acaggaaatc gaggccatcg tcggtccgta tcccatgcgg atcacgccga
     3781 cggtgctcgg cacgatcaaa gagaagggtg atccgatctg gaagcaggtg atgccggatc
     3841 ccgccgaact gaccgacatc gacgccgaag acgatccgct ggaagaagac gtgatgagcc
     3901 cggtgccgca cctggtccac cgctatccgg atcgcgtgct gctcatggtc accaatcaat
     3961 gccccatcta ttgccgcttc tgcaccagaa agcggctggt ggggaaaccc ggcttcctca
     4021 aaaaaggtga actcgatcaa gcgattgcct atctgcagga gcatacggaa gttcgcgatg
     4081 tcatcctgtc aggcggcgat cccttgctgc ttcccgacca tcttctcgag cgcatcttga
     4141 aggcgctcag gagcattccc catctggaag tgatccggct gggctcccgc gtacccggca
     4201 cattgccaga acgcattacc cccaaacttt gcagcattgt gaagaaatat catccggtgt
     4261 atatgaacct gcatttcaat catccggacg aactgacacc ggccgtcaag gaggcctgcg
     4321 gcatgttagc cgatgccggc atcccgttgg gcgcccagac cgtgctgttg aagggcgtca
     4381 acgacgatcc cgaaacgatg aagcgactgg tgcaccaatt gttgcttgct cgcgttaaac
     4441 cttattactt gtaccaagct gatttgacca agggcaccaa ccatttccga accacgattg
     4501 agtccggcct gaacatcatc aaagcgctgc agggtcacac gagcggtatg gcggtccctc
     4561 atttcgtcat cgatgcaccg ggtggcggag gcaaaattcc cctgcttcct gacgactatc
     4621 tcgtgcatat ggatgaggac ggggccgtcc tgcggaacta tgaaaacaaa accttccatt
     4681 atccgcagcc gaaagccggc aatggacgcg aattgccgat ggttggcgca cggacgtcct
     4741 atgacgattc gtcgtgcgcc gacagctatc ctgagcggga cggttattcg tcctggggca
     4801 acagctgcgg cggggaga
//
LOCUS       DASTQY010000375         3930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_882564, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000375 DASTQY010000000
VERSION     DASTQY010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882564"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..2518)
                     /gene="lptD"
                     /locus_tag="VFR82_12230"
     CDS             complement(20..2518)
                     /gene="lptD"
                     /locus_tag="VFR82_12230"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEU4502409.1"
                     /translation="MSEGYPRFGVEMAGTLARVLGLLFGIVIFNLFPVPGQAAGVAGP
                     SASAPASGSPPLEVKADRIDYLQDQDIYEAEGSVVVDQGSLHLTADHLIVQALPGIMI
                     ATGHVRLTDPKADIVAERLELNVNTEAGVITHGEVYMKASNSTIDGRLIQRFSEDHYR
                     FKEGRFTNCDAPEGETPAWRFRFKDLDLNAGDSLAFKGGWFCVADVPVIPIPTMTYPL
                     SPRQSGFLVPTPGYDNRFGFHYQQGYYWAINPSQDLTVSPSYYSNLGYGSDLEYRYVL
                     DRKSRGQWYVSYLQQTTLPNVAGVTDTPGADVKRARALVTGTHIQQITADLLLRVNAN
                     LVSDPNYLQQLSNSGAQRALPSNESNILANQRLGYGNAYLLGQYLQPLQAGGNDTFQR
                     LPEIGYSMPNLAMFDSPLLFGADANAVNFYREEGFSQNRFDIVPGLSTDVMDVGHVVG
                     FTPQAKFREVYYSRGAVDTSSQHRETFWAAMDASSKLSRRFHTATGEAMLHTIEPSVI
                     YEYVPATDQSNITQIDQVDDLPKKNLMTYMLRSRLLEQDGNSSFNWLDLTLAQSYHVG
                     AVQTRARDFTPGVEPPLGSVTQPLQPATVPVAGKKFSDLWLRAVIGNNDPQQLMVSQM
                     AGPLFSRGAGTGPLAEPVINRYVTVDAFFDPYRATVSQFNTDFRLQQSNLWYVEVGQR
                     YSREGNRVRRGDIWNPISFNEVYAPTEEIQFLTAGGAFRTPWGWTVGAKGYYDIKNGR
                     SPEYDVVALYQNPCKCWSLGLFYLQFPDRAQYNFMLSLTGIGWTESYGTNVVRTILSP
                     LVWGEKGLPWASPTGPYGRPESVQPQPRSGTVPR"
     gene            complement(2487..3779)
                     /locus_tag="VFR82_12235"
     CDS             complement(2487..3779)
                     /locus_tag="VFR82_12235"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEU4502410.1"
                     /translation="MTYDAAVSYLYNLQKHGIKLGLETMTALIARLGRPDTRYRILHI
                     AGTNGKGSTAAMTAAMLQAAGHRVGLYTSPHLVEFSERIRVDGRMISEADIGRFAEVV
                     QKAAEPDLSPTFFECTTAIALQYFAEAQVDVAVLEVGLGGRFDATNVVTPVACAVTTI
                     ALDHQEYLGTTLSSVAYEKAGIIKPLVPVVVGRMSDEARETIEQIAEERAAPVSRLGR
                     EFDVAGMPERFRFSGRTRQYDDLFCSLPGAHQVDNCACALSLIEAASERGLDVREAAV
                     REGLRTVQWPGRLEVVGRMPLVVLDGAHNPAAAAALAEYVQAFRRSNPESRVILVLGM
                     MRDKDHLGFAKPFRGLVDQVVLTQAGLSRSATVEDLYGAIHELWPSARAEAVLADALA
                     LARRFARAQDLICVTGSLMLIGDVKALRHVGRISPLRG"
BASE COUNT          834 a         1212 c         1091 g          793 t
ORIGIN      
        1 ctcattggaa cggatcggtt catcgtggga ccgttccgga tcggggttga ggttgaacgg
       61 attccggccg tccatagggg ccagtcggag atgcccacgg cagtcccttc tctccccaga
      121 caagcgggct caagatagtt ctgacgacat tcgttccgta actttctgtc caaccgattc
      181 cagtcagact caacatgaaa ttatactggg cgcgatcggg gaattggaga tagaacaatc
      241 cgagcgacca acatttacac ggattctggt acagggcgac cacatcatac tccgggctcc
      301 tgccgttctt gatgtcgtag taccccttgg ccccgacggt ccatccccac ggcgtccgaa
      361 acgcgccgcc cgcggtcaaa aattggattt cttcagtcgg cgcatagacc tcgttgaacg
      421 atatgggatt ccaaatgtca ccccggcgta cccgattgcc ttcccgcgaa tagcgttgcc
      481 ccacctcgac ataccatagg ttggattgct gcaatcgaaa gtcggtgttg aactggctga
      541 cggtggcacg atacgggtcg aaaaaagcgt ccacggtcac gtaccgattg atgaccggct
      601 ccgccagcgg cccggtacca gccccccggc tgaacagagg acctgccatc tgcgagacca
      661 taagctgctg cggatcgtta ttcccgatga ccgcccgtaa ccacagatct gaaaacttct
      721 tacccgcgac gggaactgtg gccggctgta ggggctgagt gacgctgccg agtggaggct
      781 cgacgccggg ggtgaaatcc cttgcccgtg tctgcacggc gccaacgtga tagctctgcg
      841 ctaacgtcag gtcgagccag ttaaagctgc tgttgccgtc ctgctccaat aaccggctgc
      901 gcagcatgta cgtcatgaga ttctttttcg ggagatcgtc aacctgatcg atttgagtga
      961 tgttggactg gtccgtagcc gggacgtatt cgtagatcac gctcggctcg atcgtgtgca
     1021 gcatcgcctc gccggtcgcc gtatgaaacc ggcggctcaa tttcgaactc gcgtccatgg
     1081 cggcccaaaa cgtttctcga tgctggctgc ttgtgtccac agcccctcgc gagtaataga
     1141 cctcgcggaa cttggcctga ggcgtgaacc cgaccacatg gccgacgtcc atcacatcgg
     1201 tggacagacc aggcacgata tcgaagcggt tctgcgaaaa tccctcttcc cggtaaaaat
     1261 tgaccgcatt cgcatccgcc ccgaacagca gcggagaatc gaacattgca agattcggca
     1321 tgctgtatcc gatttccgga agtcgctgga atgtatcgtt gccgccggcc tgcagcggtt
     1381 gcagatattg gccgaggaga taggcgttgc cgtaccctaa ccgctgattc gccagaatat
     1441 tggactcatt gctcgggagc gcgcgctgtg cacccgaatt gctcaattgc tggagataat
     1501 tgggatctga aacgaggttg gcattgaccc tcagcaagag atccgcggtg atctgctgga
     1561 tatgcgtacc agtcacaagc gcccgcgccc gcttcacatc cgctcccgga gtgtcggtca
     1621 cacccgcgac attcggcagc gtcgtctgct gaagataact cacgtaccat tggcctctcg
     1681 attttctgtc gagcacgtag cgatactcca aatcgctgcc ataccccaga ttgctatagt
     1741 aggacggcga aaccgtgaga tcctggctcg gattgatggc ccagtaatag ccctgttgat
     1801 agtggaagcc gaaccggttg tcataacccg gagtcggaac cagaaatcca ctttgtcgtg
     1861 gtgaaagtgg ataggtcatc gtcggaatcg gaatcaccgg cacgtccgcg acgcaaaacc
     1921 aaccgccttt gaaggcgagg ctgtctccgg cgttcaggtc tagatccttg aaccgaaacc
     1981 gccaggccgg cgtttctcct tccggcgcgt cgcagttcgt aaaccgccct tccttgaagc
     2041 gataatggtc ttcggaaaat cgctgaatca gccgtccgtc tatcgtcgaa ttcgaggcct
     2101 tcatatatac ctccccgtgc gtaatcacgc ccgcttcggt attcacattc agctccagcc
     2161 gctcagcgac aatatcggcc ttcggatccg tcaacctcac atgccctgtc gcaatcatga
     2221 tgccgggcaa tgcctggaca atcagatggt ccgccgtgag gtggagggac ccttgatcga
     2281 cgaccactga cccttccgcc tcatagatgt cctgatcttg gagatagtcg attcgatcgg
     2341 ctttcacttc gagaggaggc gatcccgaag ctggagcgga agcagacgga ccagcgacac
     2401 ctgctgcctg ccctggcacg ggaaagagat tgaagatcac gatgccgaac aggaggccga
     2461 gaactcgcgc caacgttccc gccatttcaa ccccgaagcg gggatatcct tccgacatgc
     2521 ctcaacgctt tcacgtctcc gatgagcatc aacgagccgg tcacacaaat gagatcctga
     2581 gcccgtgcga agcgtctggc aagcgccagc gcatcggcca acaccgcttc cgcccgagcc
     2641 gaaggccaga gctcgtgtat cgcaccgtag agatcttcaa ccgtggccga gcgtgagaga
     2701 ccggcttgtg tcaggacaac ctggtcgacc agtccccgga aaggcttcgc gaatccgaga
     2761 tggtccttgt cccgcatcat gcccagcacg aggatgacac ggctttcggg attcgaccga
     2821 cgaaaggcct gcacatactc agctaaggca gccgctgccg ccggattatg ggcgccatcg
     2881 agcactacca gcggcatccg acccacgact tccaagcgtc ctggccattg cacggtccgc
     2941 agtccttcac ggacggcagc ctctctcacg tccaatcctc gttcactcgc cgcttcgatc
     3001 aaagacagag cgcaggcaca gttatccacc tgatgcgctc ctggtaatga gcaaaacagg
     3061 tcgtcatact gacgcgtacg tcccgagaat cggaaccgct ccggcatgcc tgccacgtcg
     3121 aactcccgac ccaatcggct cactggcgcg gcacgctcct ccgcgatctg ctcgatcgtc
     3181 tcccgcgcct cgtccgacat ccgcccgaca acgaccggca cgagcggttt gataattccg
     3241 gctttttcgt atgcgaccga agacagcgtc gtgcccaggt actcttgatg atccaaggcg
     3301 atcgtcgtga cggcacaggc gaccggcgtg acgacgttgg tggcatcaaa tcggcctccc
     3361 aagccgactt ccaagaccgc cacatcaacc tgagcctcgg caaaatactg gagggcgatg
     3421 gcggtcgtac actcaaagaa ggttggcgac aggtccggct cggcggcctt ctgcacgacc
     3481 tccgcgaacc ggccgatgtc cgcttcagag atcatccggc cgtccactcg gatgcgttca
     3541 ctgaattcaa cgaggtgggg cgacgtatac aatcccaccc gatgtccggc cgcctgcaac
     3601 atggcagccg tcatcgcagc cgtcgaccct ttcccattcg taccggcgat gtgcaagatg
     3661 cgatagcggg tatccggccg cccaagccgg gcgatcaacg ccgtcatcgt ctcgagcccg
     3721 agtttgatgc cgtgtttctg aaggttgtag agatacgaga cggcagcgtc gtacgtcatg
     3781 ggctttcatc cgtcttccca tcacccgtgg gagcagtcca tttcccagcc ggacggtgga
     3841 ttctgaggcg acgcaggaac gacgcctgga gaaaggagcg tcgcggggta tcgattgcgt
     3901 agcaatcggt gggtacgggt gggtaagacc
//
LOCUS       DASTQY010000376         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_163086, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000376 DASTQY010000000
VERSION     DASTQY010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163086"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..489)
                     /gene="rplI"
                     /locus_tag="VFR82_12240"
     CDS             complement(22..489)
                     /gene="rplI"
                     /locus_tag="VFR82_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU4502411.1"
                     /translation="MKVILQETMEGVGHLGDLIDVKDGFARNFLLPRKKAVVANDRNI
                     KSFEHSKRVAAERAKKEKLEIETHAKKMSTVTLTVEAQVGKDDKMFGSVTVKDIAEGL
                     AEQGYTVDRRKIQLPHPIKELGSFTVPVKLPREVTAAVTVRVVKKQEAETATT"
     gene            687..2138
                     /locus_tag="VFR82_12245"
     CDS             687..2138
                     /locus_tag="VFR82_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502412.1"
                     /translation="MTARELIEVWTTQLTAEHHQLRDSETDRPITPVTGRLVQTVGTL
                     YLYEFKLPPDRSVIIDTPVSIVPDDGMEPTEGFVLSHRDGVVLVQTFDAVGSTITNAT
                     IVPDRGSFLMAAAERFAQMLSQPDSYRLGPADRLAHVFEASSGSEQLGGSSASVLTTI
                     WSEDPALRQQKVAAMAIELIRTNKRILLVSPDHDAADAISGIIAKAMKAGGLVYKTWV
                     SRYEMTLTYQASGLPIHELGFEAQMHQFYAKSRADKAALRRKYERFRELTPILAYKGH
                     KQKDLDEVRLLEWRLLTQLSDLQAKSQEARSTLEAYETLPLLRRLTMQAVGKNVESLH
                     QYLALYEAQMASLRKELEIAKARIDELVPEAAVPKDMRPEFEDLKEEIVRLGGTKKIR
                     ELLAAEEDTNRQAFIQNRRLVVTTADRVLGDPLFSRVRFDVLIADNAPWISPHALLGA
                     AGLVRERIVLTGDQRHISTAGLWKSVQIAGSQR"
BASE COUNT          506 a          627 c          648 g          496 t
ORIGIN      
        1 acatgaaggc gcctcgtttt gttacgtggt agcggtttcg gcttcctgtt tcttgaccac
       61 gcgcacggtg actgccgcgg tgacctcacg cggtagtttc accggaacgg taaagctgcc
      121 caactcttta atgggatggg gcagctgtat tttgcggcga tctactgtat agccttgctc
      181 cgcgaggccc tcggcaatgt ccttgaccgt gaccgagcca aacatcttgt cgtccttgcc
      241 gacctgagcc tcgaccgtga gggtgacagt ggacatcttt ttcgcgtgcg tttcgatctc
      301 gagtttttct ttcttagctc gttcagccgc cacacgcttg ctgtgctcga acgatttgat
      361 gttccggtcg ttggcaacga cggccttctt gcggggaagc aggaagttcc ttgcaaatcc
      421 gtctttaaca tcaatgagat cgcccagatg gccgacccct tccattgtct cttgcaaaat
      481 gaccttcatg cctctaactc cttctgttgg aaagggaaag aggatactgg aggggccgaa
      541 ggaaagtcaa ttgcagatct tcgaaaatga cgaatttttg ccgccttggc gatggatctc
      601 gacgctgtca cggtcactat ccaaccgctg gactctcgtc gtccagcgcc cgtaagatgg
      661 ccgcagggtc cgttgagaag acgatcatga ctgcccgaga gctcatcgaa gtttggacca
      721 cacaactaac cgctgagcac caccaacttc gcgactccga gacggataga ccaattacgc
      781 cggtcaccgg gcggctggtg cagactgtcg gaacactgta cctttatgag ttcaagctcc
      841 cgcctgatcg ctcggtgatc atcgatacgc cggtgtctat cgttccggac gacggcatgg
      901 aaccgactga aggcttcgtg ttgagccatc gggatggagt ggtcttggtg caaacgttcg
      961 atgcggttgg atcgaccatc accaatgcca caatagttcc cgatcgggga agctttctca
     1021 tggcagcagc tgaacggttt gcgcagatgc tgtcgcagcc cgattcctac aggctggggc
     1081 ctgcagaccg gctggcccat gtctttgaag cgtctagcgg atcagagcag ctgggcggct
     1141 catcggcctc tgtcctcacg acgatatggt cggaagatcc ggccctgcga cagcagaaag
     1201 ttgcggccat ggcgatcgag cttatacgca cgaacaagcg catattgctt gtcagtcccg
     1261 atcatgatgc ggcggatgca atcagcggca tcatcgcgaa ggccatgaag gccggtggct
     1321 tagtctataa aacgtgggtc agccgttatg aaatgacatt gacgtatcaa gcgtcgggcc
     1381 tgcccattca cgagctagga tttgaggccc aaatgcatca gttttatgcg aagtcgcgtg
     1441 ctgataaggc cgcgctccgt cgcaaatatg agcgcttccg cgaactcacg ccaatcttgg
     1501 cttacaaagg gcacaagcaa aaggatttgg atgaagtccg gctgctggag tggcggttgc
     1561 tcacacaact cagcgatctg caggcaaaaa gccaagaagc gcgctcgaca cttgaggcgt
     1621 atgaaacctt accgctgcta cgccggctca cgatgcaagc ggtgggcaag aatgtcgaat
     1681 cgctccatca atatcttgcc ctctacgaag cccaaatggc ctccctccga aaagaactcg
     1741 agattgccaa ggctcgtatt gacgaactgg ttcccgaagc cgcagtgccc aaagacatgc
     1801 gacctgaatt tgaggaccta aaggaggaga tcgtccggct gggcgggacg aaaaaaattc
     1861 gcgagctgct ggccgccgaa gaagacacca atagacaagc gttcattcag aaccggcgtc
     1921 tcgtcgtgac gaccgccgac cgcgtgctgg gcgatccgct gttcagccgg gtgcgatttg
     1981 atgtcctcat cgcggacaat gcaccatgga tttcccctca cgcgctgttg ggggcggctg
     2041 ggctggtacg cgaacggatt gtgctgacgg gagatcaaag acatatttcg acggccggtc
     2101 tatggaaatc cgtgcaaata gcagggagtc agcgctaagc cccgtgagac ttgcttgacc
     2161 taagcgagtc gaattcagga taatcgcgtt ccctgtccat cccggcgcgt gaagtggttc
     2221 aacgaaggga gcctgcaaac ctgccgacat cgcggtgaac caggcgactg gaacgac
//
LOCUS       DASTQY010000377         5232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_883813, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000377 DASTQY010000000
VERSION     DASTQY010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883813"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VFR82_12250"
     CDS             <1..234
                     /locus_tag="VFR82_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU4502413.1"
                     /translation="FCDIYCLEPGQEQIGHVHGSQDKVYLVLEGKGTFQVGSDKQVLG
                     PGQGTMAPAGEEHGVKNDSGQRLKVLVFVAPNP"
     gene            complement(252..1643)
                     /locus_tag="VFR82_12255"
     CDS             complement(252..1643)
                     /locus_tag="VFR82_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU4502414.1"
                     /translation="MSATIFVTDDEPAIRSALVKRLSRRQHRVTAFESGETLLHALDH
                     EVPDLVLLDIKMPGLSGLETLKACRSKAPFALVIMLTAYGTVQDAVEAMKLGAYDFVI
                     KTVDLDGIEQVLDRALELSALRRRLSYQSEQDRDQYAMDHLIADSAAMKSLLEQIREV
                     AHNPKSTVLLLGETGTGKEFVARVLHHNGSRASGPFIGVNCTAIPRDLFESELFGYER
                     GAFTGAHQRKLGLLDRAEGGTLFLDEIGDLDLTMQAKLLRVLQERTFRRLGGTTDIAA
                     DFRLITATNRDLKKAITTGAFREDLFFRLNVVALELPPLRQRIEDIFPLSMKALLHYG
                     TEFGKDVTEIDAEARALLECYHYPGNIRELQNIIERATIFCTGRTLSANYLPRELRDQ
                     AKQTATVVPHGDGQVIRVEMQVGKHTLGEIEDSIIEETLRLADYNKSLAAKQLGLTRF
                     ALDRRLKKIRKDE"
     gene            complement(1640..3571)
                     /locus_tag="VFR82_12260"
     CDS             complement(1640..3571)
                     /locus_tag="VFR82_12260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4502415.1"
                     /translation="MRRLLKLFSDLPIARKLLLASVIPVLTVVSLSIVTYRSVETFSE
                     DESELNTLYLSQRLAAEYLRLVVDLETGFRGFVITKHEKYLYPYRTAQDHIQAVGISL
                     DEQVRRYEEQRALLSNVRTLVAQFMNEKEALIDAMKAGRRDEAKRYIEEGRGRMIMVQ
                     IRDEMAHFDRARQQTLDTLLSRLSQDRSTMLSVILGGGTLALCLMLLALHLIARSITG
                     PLVSLAKVVSSSPAGVAPTVPVINRKDEIGNLTQVIHTMSTQIRAHLAMVERSEAELR
                     SVNEDLTASEAKYRSLVDLAPFGIFSTKGFDITFSNRHNRILAGLDPDGADDPEAFRQ
                     RIHPEDRERVLGEFARAVEERKTCETVFRFLHADGTVRKVLSRRTPIEQDSNQTVIYQ
                     GFNIDITALDDMQHQLSRAERLATLGQVAAGIAHEIRNPLVGIGSTAALLRDEFDPSD
                     DKRSDLDIILNETRRLDRIVNQIIDYARQRDLVPTTWPVDGLIEEVLKLLDTRLETQQ
                     VTVTRSCTTNLTAYADRDQIKQVMLNLCHNSLDAMLGGGELRIGAGPSTRGGHGGIFI
                     EVTDTGSGISQKDLAQVFQPFFTSGKRHGTGLGLAICRNITEAHGGDMTLTSEPGHGT
                     TARLWLPLRPVSDVVGTLG"
     gene            complement(3639..>5232)
                     /locus_tag="VFR82_12265"
     CDS             complement(3639..>5232)
                     /locus_tag="VFR82_12265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEU4502416.1"
                     /translation="RILHMKHIAWLEIWFLSLLSLTACSDLFPTLDLSPPYQPPEYVV
                     PASWHGSSPFVEAKPSDGDLRPDWWTLYDDPALNTLIDQAMAANPDLEAAAERFVQAR
                     DMMMKARSQYLPRLGLGFGASDNRASVNALFFPRDIPQQASSVSLGGLASWEPDFWSA
                     LRNATRIETYRAEERAADYGLARLSLQAEIGANYFLLRGYDAQIAIYTQSIELYRRSL
                     DIVKAQFAGAIASALDVARVESLLFTTETQLARVQGQRQVTEQAIAILVNMAPASFTI
                     KPKAAEDLRVASFTLPRALPSTLLERRPDIARMERQMAQASRAIGIARAAFFPDVRFR
                     AGGGFEDAGFNLLSLANSFWSYGAAVSMPLFQGGFRRAQLQQSWSAYRETEDRYRSTV
                     LNAFREVENNLSLTSQLTLAANRQDAAVGATFKAQHLTTELYQGGLASSLELIYAQVA
                     TLTARIESVQIKAELLQSSVGLIRALGGGWNRNQLPADEQIQPFGTFQYVNLSKPTPL
                     KGIDVNADNNAIHNDLTKPVLP"
BASE COUNT         1074 a         1453 c         1583 g         1122 t
ORIGIN      
        1 ttctgcgata tctattgtct cgaaccgggg caggaacaaa tagggcatgt gcatggttcg
       61 caagacaaag tctaccttgt cttagaaggg aaaggcacgt ttcaggtcgg ctctgataag
      121 caagtgctgg ggccgggcca aggcacgatg gccccggccg gtgaagagca cggggtgaaa
      181 aacgacagcg gacagcgatt aaaagtattg gtctttgtgg caccgaatcc gtgacggttg
      241 ttgttgcgca gctactcgtc ttttctgatc ttttttaggc ggcggtcaag cgcgaatctg
      301 gtcagtccca gctgtttcgc cgcgaggctc ttattgtaat cggcaaggcg cagggtctcc
      361 tcgatgatgc tgtcttcgat ctcgccgagt gtgtgcttgc cgacctgcat ctcgaccctg
      421 atcacttgac catctccatg cgggacgact gtagctgttt gtttggcttg atcgcgcagt
      481 tcacggggga gatagttcgc gctgagcgtt cgtcccgtgc aaaagatcgt cgcacgctcg
      541 atgatattct gcagctcgcg gatgttgccg ggatagtgat agcattctaa caacgcacga
      601 gcctccgcat cgatctcggt cacgtccttg ccaaactccg ttccgtaatg cagcaacgcc
      661 ttcatgctca aggggaagat gtcctcgatt cgctgtctca gcggcggcaa ttcgagtgca
      721 acgacgttca gccggaaaaa aagatcttcc cgaaaggcgc ctgtggtaat ggcttttttc
      781 aggtcacggt tcgtcgcagt gatcaatcgg aaatctgcgg cgatgtcggt cgtgccgccg
      841 agccgccgga aggtgcgttc ctggagcact cgcaacagct tggcctgcat cgtcaaatct
      901 aaatcgccga tttcatccag gaacagggtt ccaccttccg ctcggtcgag caggccaagc
      961 ttgcgctggt gcgctccggt gaaggcgccg cgctcatagc cgaacaactc gctttcgaag
     1021 agatcgcgtg ggatcgccgt gcaattcacg ccgatgaacg ggccggaggc gcggctgccg
     1081 ttatggtgca gtacacgggc gacaaactcc ttgcccgtgc cggtttcgcc caacaagagc
     1141 acagtgctct tgggattgtg cgcaacttcc cgaatttgct caagaaggct cttcatcgcc
     1201 gcgctgtcgg caatgagatg gtccatggca tattgatccc tgtcctgttc gctttgatac
     1261 gaaagacggc gtcgaagtgc ggacagctcc agcgcccgat ccaggacctg ttcaattcca
     1321 tcaagatcga cagttttgat gacgaaatcg taggcgccga gcttcatggc ctccacggcg
     1381 tcctgcaccg ttccgtaggc ggtgagcatg atgacgagtg caaacggcgc tttggagcgg
     1441 caggctttga gggtttcaag accggagaga cctggcatct taatatccag aagcacgagg
     1501 tccggtactt cgtgatccag ggcgtgcagc agagtttcac cggattcaaa cgccgtcacg
     1561 cgatgttgtc gccgtgacag acgcttcaca agggctgagc ggatcgcagg ctcatcgtcg
     1621 gtgacgaaga tggtggcgct catcccagcg tccccacgac gtccgacacg ggccgcaacg
     1681 gcagccaaag cctcgccgtt gtgccgtggc ccggctcgct cgtcaacgtc atgtcaccgc
     1741 cgtgcgcctc ggtaatgttc cggcaaatgg ccaagcccag gccggtgcca tgccgtttgc
     1801 cggatgtaaa aaagggttga aacacctgag cgagatcttt ctgactgatg cctgagcccg
     1861 tgtcggtcac ctcgatgaag atgcctccat ggcccccgcg tgttgacggt ccggctccta
     1921 tacgcagttc gccaccgccc aacatggcat cgagtgagtt gtggcaaaga ttcaacatca
     1981 cctgtttgat ctggtcgcgg tccgcatagg cggtcagatt cgtagtgcag gaccgcgtga
     2041 ctgtgacctg ctgggtttcg agccgtgtgt ccaacaactt gagcacctcc tcgatgaggc
     2101 cgtctactgg ccacgtagtc ggtacgaggt cccgctgacg ggcgtaatcg atgatctgat
     2161 taacgatgcg atcgagccgc cgggtttcat tcaagatgat gtcgaggtcc gatcgtttgt
     2221 cgtcggacgg atcgaattcg tcccgcaaca gcgcggcagt ggagccgatc ccgaccaacg
     2281 gattgcgaat ctcatgcgcg ataccggcgg ccacttgtcc cagtgtggcc aagcgctcgg
     2341 cgcggctcaa ctggtgctgc atatcgtcaa gggcggtgat gtcgatgtta aagccttgat
     2401 agatgactgt ctggttcgag tcctgttcga tcggtgtgcg gcggctcagc actttccgta
     2461 ccgtgccgtc ggcatggaga aacctgaaga cggtctcaca cgtctttcgc tcttcgaccg
     2521 cacgggcgaa ttctccgaga acccgttcac ggtcttcagg atgtatccgc tgcctgaagg
     2581 cctccggatc atcggcccca tcgggatcga ggccggctag gatccggttg tgacggttgc
     2641 tgaacgtgat gtcgaatcct ttggtagaga aaatgccgaa aggagcaaga tcgaccagac
     2701 tgcgatactt cgcctctgat gcagtaaggt cttcgttgac gctgcgcagt tcggcttccg
     2761 atctctctac catggcaaga tgagctcgga tctgagtgct catcgtatgg atgacctggg
     2821 tgagattgcc gatttcatcc ttacgattga ttaccggcac cgtcggagca acgcccgccg
     2881 gggaggaact caccaccttt gccagcgaga ccagcggtcc ggtaattgag cgggcaatga
     2941 gatgaagggc caggagcatg aggcacaatg cgagcgtgcc gccgccaagt atgaccgaca
     3001 gcattgtgct ccggtcttgc gatagccgcg acagcagagt gtccaaggtt tgttgccgcg
     3061 cgcgatcaaa gtgggccatt tcgtcccgga tctggaccat gatcatccgg ccccggcctt
     3121 cttcaatgta acgtttagct tcgtccctcc ggccggcttt catggcgtcg atcaacgcct
     3181 ctttttcgtt catgaactga gccaccaacg tgcggacatt tgagagcagc gcccgctgct
     3241 cttcgtatct ccgcacttgc tcgtcaagcg agattccgac ggcctgaatg tgatcctgtg
     3301 cggtccggta tgggtacaga tatttttcat gtttggtgat gacgaagccg cggaacccgg
     3361 tctcaaggtc gacgaccaag cgcaaatatt ccgccgcaag acgttgtgag aggtataggg
     3421 tattgagttc gctctcgtcc tctgaaaacg tttcgacact tcggtaggtg acgatgctga
     3481 gcgagacgac cgtgaggaca ggaataaccg aggcaagcaa cagcttgcgc gcaattggaa
     3541 ggtcgctgaa cagtttcaga aggcgccgca tgaaagcctc ctgaggtcat cgtagttgca
     3601 ggcctgacgc tgagtcaaac cggtcacgaa cgattcgttc acggaaggac gggcttggtc
     3661 agatcattgt ggatcgcgtt attgtcggca ttgacatcga ttcccttgag gggcgtgggt
     3721 ttgctgagat tgacatattg gaacgttcca aacggctgga tctgttcgtc cgcgggcaat
     3781 tgattccggt tccaacctcc gccgagggca cgaatcagtc ccaccgagct ttgcagcaac
     3841 tcagccttga tctgcacaga ctcgatgcgg gcggtcaacg tggcgacttg tgcataaatg
     3901 agttcaaggc tggatgccag accgccctga tagagctccg tcgtcaggtg ctgtgccttg
     3961 aaggtggcgc cgaccgcggc atcctgccga ttcgcggcga gggtcaactg gctcgtcaaa
     4021 ctgaggttgt tttcgacttc gcgaaaggcg ttgagcacgg tcgatcggta tcgatcttcc
     4081 gtctcgcggt acgccgacca ggactgttgc aattgcgcgc gacgaaatcc gccctggaag
     4141 agcggcatcg agacagcggc accgtaggac caaaaactat tcgccagcga gagcagatta
     4201 aacccggcgt cttcaaagcc gccgccggcg cggaacctca cgtcgggaaa aaaggcggcg
     4261 cgggcgatgc cgatggcccg gctcgcctgg gccatctgac gctccatcct ggcgatgtcc
     4321 ggccggcgtt caagcaaagt cgacgggagc gcccgcggaa gggtgaaact cgccacgcgg
     4381 aggtcctcgg ctgctttggg tttgatggtg aaactggccg gcgccatatt gacaaggata
     4441 gcaatcgctt gttcggtgac ttgtcgttgt ccttgtaccc gtgcgagctg cgtctcggtg
     4501 gtaaacaaca gagattcgac ccgggccacg tcgagcgcgg aggcgatcgc gccggcgaat
     4561 tgggctttga cgatgtccaa cgaccgtcga tagagttcga tcgattgggt ataaatcgcg
     4621 atctgcgcgt catagcctcg cagcaggaaa taatttgccc caatttccgc ttggaggctg
     4681 agacgtgcca gtccatagtc ggcggcgcgt tcttcggcgc gatacgtctc aatgcgggtg
     4741 gcatttcgaa gggccgacca gaagtcgggc tcccaggagg caagcccacc aagggaaacc
     4801 gacgaggcct gttgcggaat atcgcgcggg aaaaacaatg cattgaccga ggcacgattg
     4861 tccgaagcgc cgaaccccag acccaggcgc ggaaggtact gggaccgggc cttcatcatc
     4921 atgtctcggg cctgcacaaa ccgctcggcg gcagcctcca gatcaggatt agccgccatg
     4981 gcctgatcaa tgagggtatt tagggccgga tcgtcgtaca gcgtccacca atctgggcgc
     5041 aggtcgccgt ccgagggttt ggcctcgacg aatgggctcg acccatgcca tgaggcaggg
     5101 accacatact ccggtggctg ataagggggc gagagatcga gagtcgggaa cagatcgctg
     5161 catgcagtga ggctgagaag agatagaaac cagatctcca gccaggcgat atgcttcatg
     5221 tgcaggattc tg
//
LOCUS       DASTQY010000378         4167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1488533, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000378 DASTQY010000000
VERSION     DASTQY010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488533"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFR82_12270"
     CDS             <1..294
                     /locus_tag="VFR82_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU4502417.1"
                     /translation="VPIVTRDDVRFAKPDPDLFLAAGQRLNVPMSRCVIVGDSVWDLL
                     AARRAFGLGVGLLSGGYGREELERAGAYRVYQDPAELLKHLDEVGVRLDRRGE"
     gene            331..792
                     /locus_tag="VFR82_12275"
     CDS             331..792
                     /locus_tag="VFR82_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU4502418.1"
                     /translation="MPKKLSAGIVFYRRGEKGIEVLLVHPGGPFWAKKDDGAWSIPKG
                     EYLEGEDPLTVAKREFHEETGTEVSGACVALTPAKQPSGKVITAWAVEGDLDPTAVQS
                     NTFSMEWPPKSGRVQSFPEVDRALWCDLALARKKLLIGQRVLLDELDQLVR"
     gene            complement(1185..2039)
                     /locus_tag="VFR82_12280"
     CDS             complement(1185..2039)
                     /locus_tag="VFR82_12280"
                     /inference="COORDINATES: protein motif:HMM:NF039714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HEU4502419.1"
                     /translation="MKIIRAGRVLLIPLLLASLTFAGCAATTLLYNHADWLIARQIDG
                     YFDLTRPQKTFVSSRLTGILSHHRHEALPRYESVLRQATARVQDGVSTDDLDWAFVQY
                     DQLRADLFGRFVSDGTEFVRQVNDSQVSRLKKALQSRLARQEALLQDDPGKRLAKRTE
                     RILGLVRDWLGPLSTQQEQEITHLTMHFPDSLPVWYAHQKQRHDQLLALVESRSSDQT
                     PARLEDWLVNQDKDSDPHFAEMTKQLRRHITDLIMSLDRLATPAQRQHFLAKVDDLAA
                     TIRRLQAA"
     gene            complement(2169..2939)
                     /locus_tag="VFR82_12285"
     CDS             complement(2169..2939)
                     /locus_tag="VFR82_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU4502420.1"
                     /translation="MQAFVGALCAAHPAVAIPVTALLLVVIWFGINWAYHATKKPSEI
                     LFPLDNAFDKNLTETWHEYGPLFREHATAIITPELLAALAQVEGSGNPLARTYWRWHS
                     SWNPLQWYRPASSAVGMYQITSGTFQAAKRYCIHDHVVAEDGPWHDVRSCWFNELYTR
                     VLPSHAIELTAALLDRQVAQAIGSRSGVTVQRKQDVAAIIHLCGASVGQAYVSRGFQL
                     LQRQHCGEHEVRNYLRKVSAAKQQFARLAAHTQTERWK"
     gene            complement(3469..>4167)
                     /locus_tag="VFR82_12290"
     CDS             complement(3469..>4167)
                     /locus_tag="VFR82_12290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU4502421.1"
                     /translation="TEQRERQRLAADLHDYLGQLLALTRIKLGLAKQQPMPPPLAKII
                     TDVEEVTNKAIVYTRTLISQLSPPALSASGLPMALQWLTEQMQQHDLSVSLQVKTKIP
                     TIPEDQALLLFQSVRELLFNCVKHAESHEATITLEQVDESLYIQIADWGVGFDLASPT
                     KKECSPTSGFGLLSIRERMLSLGGHFELASSPGNGTTAMLVLPLGDSAVDSFMPSHRL
                     LMKEPVRATNRDQT"
BASE COUNT          896 a         1084 c         1307 g          880 t
ORIGIN      
        1 gtgccgatcg tcacgcgtga cgatgtccgt tttgcgaagc cggatcctga cttgtttctt
       61 gctgcgggtc agcggctcaa cgtgccaatg agtcggtgcg tgatcgttgg agacagcgtc
      121 tgggacttgc tggcggcccg ccgggcgttt ggccttggag tcgggctgct ctcgggtggg
      181 tatggacgcg aagaattgga acgggccggc gcgtataggg tgtaccagga tccggcggaa
      241 ttgttgaaac atttggacga agtgggggtg aggttggata gaagaggaga gtaggcatat
      301 caaggactct tgaggagaac gccagcatcc atgccaaaaa agctgagcgc ggggattgtg
      361 ttctatcgca gaggtgagaa agggatcgaa gtgttgctgg tccatcccgg cggacccttc
      421 tgggctaaaa aggatgacgg agcctggtcg atccctaaag gtgagtacct ggaaggagaa
      481 gacccgctga cggttgcgaa acgggaattt catgaggaga cagggacaga agtgagtgga
      541 gcctgtgttg ctttgacccc ggcaaagcag ccgagcggga aagtcattac ggcgtgggcc
      601 gtggaaggag acctcgatcc gaccgcggtt caaagcaaca cattctcaat ggagtggccg
      661 ccaaagtcgg gccgggtcca gagctttccg gaagtggatc gcgccctttg gtgcgacctt
      721 gcgttggcga gaaagaagct gctcataggg caacgcgtcc tgttggacga gctggatcag
      781 ctggtgagat gattgagatg aacgtgatgc ctgaccggct gcaagtcagc gccgcgagct
      841 caggcacgat cctgaacgat tccaactcag cgagtaaccg acgaatcgca cttgtgcttc
      901 tcgcacaaag gatgagtcgt gtcgtgcaat tctttcgcgt agtacgaaac aatatcgcac
      961 tcttccgccg acagctcgcc gctctgaagc gctaagccga tgagtcgttc acacatccgc
     1021 ataaaatctt taatttcagt tcgcatcgcc cgcggaaatc tacaggacca gggttctcgc
     1081 cagtactggg aacaccccta gttttactct gacactgcat cgccaggaac ccgctgccgt
     1141 ggcgggctgg ccctgaatgc ttggttgccg ggcaagtgaa aggtttaggc agcttgtaaa
     1201 cgccggatgg tggctgcgag gtcgtcgacc ttggcgagaa agtgctggcg ctgtgccggc
     1261 gtggccaagc gatccaggct catgatcaag tccgtaatgt gccgacgcag ctgtttcgtc
     1321 atctcagcga agtgcggatc ggagtccttg tcctgattca ctaaccagtc ctccaggcgc
     1381 gccggggtct ggtcactgct gcgagattca accagcgcga gcaattggtc atggcgctgt
     1441 ttctgatggg cataccagac gggtaagcta tcgggaaagt gcatcgtcag gtgagtaatc
     1501 tcttgctcct gctgtgtgga aagcgggccc agccagtctc tcaccaagcc gagaatccgc
     1561 tcggtccgct tggcgaggcg tttgccggga tcgtcctgca gcagggcttc ctggcgtgcc
     1621 agccggctct gtaatgcctt cttgagtcgg ctgacctgcg agtcgttgac ctgacggaca
     1681 aattctgttc catccgagac gaagcgaccg aagagatccg cccttagctg atcgtattgg
     1741 acgaaggccc aatccaggtc gtccgtcgac accccgtcct gtacgcgcgc agtggcttgg
     1801 cgcagcacgg attcatagcg gggcagggct tcatgtcgat ggtgcgataa aataccggtc
     1861 agcctcgatg atacgaacgt cttctgtggg cgggtgagat cgaagtaccc gtcgatctga
     1921 cgcgcgatca accaatcggc gtggttatag agcagtgtcg tggcggcgca gccggcgaac
     1981 gtcagggatg ctaacaagag gggaatgagc aggacgcgtc ctgcacgaat gatcttcatc
     2041 ggttgtgatt gcctaacgat cgggaaacgg tgagtgtact atcacggtaa cacaagagcc
     2101 tgccctgctg ctagtcagtc agcgacgagg actttctctt tcttcaacag gtgtttacaa
     2161 gacattcatc acttccacct ttctgtttga gtatgtgcgg caagtcgagc gaactgctgt
     2221 ttggcagcgc tgaccttccg gaggtagttc cttacctcgt gctctccgca atgctgcctt
     2281 tggaggagct gaaacccgcg tgagacgtac gcttgtccga ccgaggctcc gcacaagtga
     2341 atgatagcgg caacgtcttg cttccgctgt acggtgacgc ctgatcgtga cccgatcgct
     2401 tgcgcgacct ggcgatccag cagggccgcc gtcaactcga tggcatggct gggcagcaca
     2461 cgggtataga gttcattgaa ccagcaggaa cgcacgtcgt gccagggccc gtcttctgcc
     2521 accacatgat cgtgaatgca gtaacgctta gcggcttgaa acgttcctga ggtgatctgg
     2581 tacatcccga cggcgcttga ggctggtcgg taccattgca gggggttcca ggaggagtgc
     2641 caccgccagt acgtgcgggc aaggggattg ccgctgcctt cgacctgggc cagggccgca
     2701 agcaattcgg gtgtgatgat ggccgtcgca tgttcacgaa acagggggcc gtattcatgc
     2761 cacgtttcag tgagattttt gtcgaaggcg ttgtccaatg gaaacaatat ttcgctgggt
     2821 ttcttggtcg catgatatgc ccagttgatg ccaaaccaga tcacaacaag cagcagggca
     2881 gtgacgggaa tggcgaccgc cggatgagcg gcgcagaggg cgcccacgaa ggcttgcatt
     2941 tcacgccagc gccaacggat gaagcgccac agcttcttac tggtaaagcg gcggcgtgac
     3001 tgcgggcggc gaggggcgcg acgattggag cgaggagaca tggaaacata taatagccga
     3061 atcacggaaa aatggatcaa ggaatgcaag atcaatcatg aggatgacaa gcggtaagtc
     3121 ctgcctgctg taatgctcga ctggcaaccg ggtgcaacgg cccggcttgg acacgagttg
     3181 cgttatcgcg gtggccaacc ggaacggcgg acattctcac ttcgcatggc gaacgctgtg
     3241 gcggcggtgc ggtactagag gtgtgcatca atcgaaaaca gacactgcag cttctttgct
     3301 ttggaggaaa gcaggattga ggcaagaaat tgaaggcagc atctggtttg tgtgttccga
     3361 aatgcgaacg tgtcgccccg actcttcgac gagcgtacgg cgcgtcgccc atggtcgggt
     3421 cagcgcgact gctagctgta gacgctcggc ggcttcaccg cgctggcgtc aagtctgatc
     3481 gcggttggtt gcccgcactg gctctttcat gagcaacctg tgagagggca tgaaagagtc
     3541 gacagcactg tcacccagcg gtaggacgag catcgccgtt gtgccgttcc ctggcgaaga
     3601 ggccagctca aaatggccgc ctaacgacaa catccgttcg cggatgctca ggaggccaaa
     3661 cccggaggtc ggagagcatt ctttctttgt tggggacgcg agatcaaaac ctacgcccca
     3721 gtccgcaatt tgaatgtata acgattcgtc aacctgttcg agcgtaatgg tcgcctcatg
     3781 cgactcggca tgcttgacgc aattgaacaa cagctcacgg accgattgaa acagtaacag
     3841 cgcttgatcc tcagggattg tcggaatctt cgtcttgacc tgtaacgaga cggagagatc
     3901 gtgctgctgc atttgctccg tcaaccattg cagggccatc ggcaggccgg acgcggatag
     3961 agccggtgga ctcagctgcg aaatcaaggt tcgtgtgtag acaatcgcct tattagtgac
     4021 ttcttccaca tcagtaataa tcttcgcgag gggaggcggc ataggctgct gtttggccag
     4081 cccgagttta atccgggtca aggccaggag ctgtccgaga tagtcatgga gatcggctgc
     4141 cagccgctgg cgctcacgct gttcggt
//
LOCUS       DASTQY010000379         5731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1066348, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000379 DASTQY010000000
VERSION     DASTQY010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066348"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFR82_12295"
     CDS             <1..204
                     /locus_tag="VFR82_12295"
                     /inference="COORDINATES: protein motif:HMM:NF013038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HEU4502422.1"
                     /translation="VGLVCMDMTMVDVTDLPSVRIGDTVTVIGQDGNDAIWADEIAQW
                     SETIPYEVLCAIGPRIPRVYTSP"
     gene            complement(340..1710)
                     /locus_tag="VFR82_12300"
     CDS             complement(340..1710)
                     /locus_tag="VFR82_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEU4502423.1"
                     /translation="MLVRIALVAALTVYVFPLLYGPVLAQVPRIQGQGTAASGMGNAF
                     SAQADDPSALHYNPAGMTQLRGVEFLTGALLSGGTTTFTSPTGVSAKGDRDGSLAWPP
                     PTHLYLTANLKDLGIRALGDLTAGVGVTVPFGSVTRWPNDGPFRTATTFNTLPLLDIK
                     PTVAYKLTDDLSIGLGADIYTFSGLFGEGHVERQSVWPGGGGIPAGAQIELFGADTAA
                     GFNASLLYTALRNPDGKPLANIGIVYRSQATLHLDGALLANGAKVSDATATFVLPQVI
                     TGAVAIWPVRNREREWKLEVDVDYVGWKSVRNLDVHLANGGTIPQPLNWRSTYAVMIG
                     TEHRWLTLESLPSWEVALRAGYTTQPTQMPDFGFDPGVPSADVHIVGAGLGLVCKQQG
                     KFLGLFTCGEVGVGVLKPKLIGLDLSYQAALYEDRTVVGNRNPTVNGLYQTTLHTGGI
                     SIRVAF"
     gene            complement(1897..4848)
                     /locus_tag="VFR82_12305"
     CDS             complement(1897..4848)
                     /locus_tag="VFR82_12305"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4502424.1"
                     /translation="MHNGAAQDLRPGPPTIVLYPKYQTILEREYMEFRPFGVDEQGKK
                     IRDLTGMSIRATVLYLESSMTRRHGEAAGLQAVQELCSSLNKRIKNPVYHVTPAFLKN
                     AWNSYSYEFTAYLYEFCEQISGDPQFVFRGGMEKASPIMQVLARPFSLSQIYGMFPYF
                     GNKFASGSIECRVVAVTNRSATLAMRFSERTLRQFGSYRRRCTFVMCQAAQGIMAAVP
                     ARVHGLPPATLTECSCIANDDDWCQWDITWDAEPSDGWARRLWSRGVGSRPAIPEESS
                     PILSPVQMDALVPSAGLQADAKVAQDHHPIRLQDGAPSLGDSSPQKQAWSRPPVSKSK
                     TDIAGYVWALSGAALTSGIVRLVYPSMSLGEVLLAGLIPVLVAGTWINWRLRKESQRR
                     EALIQEQINFVETRHEELREAYLEQEHTRVELRRTVNHLTALHQAGLLFSSTLDREAL
                     LEKVLETLIHDLHFDRAMISFYDPVRRMTMDARVAGVSREVQELARMHETPVDDPESL
                     EGVVLLQGKPLLIGDVQPYVARMHPHNRRLLQLTGSKSLLAVPLKTKDRILGSMIVDR
                     MQEHGLTQDDLELMVTFAHQVAIALDNASAYQQIEELNVGLEAKVQERTRELEQADRL
                     RSQFLSHVSHELKTPMTSIKGFLQNMLDGLTGSINEKQQRYMSRMLENSERLIRMIED
                     LLDRTRIQTGKLELAPVELDLGQCVADVVEQLRPLVQAKRQTLEATYPDVPLVVWADR
                     DRLVQIVINLVQNAMKFTPEEGRITVTVGNDNHRHAGVAVRDTGPGIAPEFRDKIFDP
                     FFRITQTRSTVKGLGLGLSIVRTLVELHGGTIEARNPDGKGAELYFTLPLRFPVEVIA
                     PTVSRRAPRILVVDDDKDIRQLLDDRLMAMGYEVETETDGLRALKAIGTGRFSGVILD
                     IGISSVDGMDVLKQIRQDDEHIPIIMVTASGAKESAIRAISLGAQAYLLKPFDVEELR
                     QVMQSFIRTAE"
     gene            complement(4930..5238)
                     /locus_tag="VFR82_12310"
     CDS             complement(4930..5238)
                     /locus_tag="VFR82_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HEU4502425.1"
                     /translation="MVLLEFSMSPLGKGESVSKYVSRSLDIIDKSGVAYRLNPMGTVL
                     EGEWDEVFAVVRKCYERMKKDCNRISCSIKVDYRKGHSGRLVSKVASVEKTLKRSIRQ
                     "
     gene            complement(5439..>5731)
                     /locus_tag="VFR82_12315"
     CDS             complement(5439..>5731)
                     /locus_tag="VFR82_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HEU4502426.1"
                     /translation="AVVEAGHARVAISTSGMAPALSGFMKEDLERILDAEFVAFVEWL
                     GQLREQAKSNEPDVEKRRTMLREALDGFRLHGKVQYPKVWLEERDKTKARTG"
BASE COUNT         1186 a         1783 c         1551 g         1211 t
ORIGIN      
        1 gtgggcttgg tgtgcatgga catgaccatg gtcgatgtca ccgatctccc gtcggtacgt
       61 atcggtgata ccgtcacagt gatcggacaa gacggaaacg atgcgatctg ggccgacgag
      121 atcgcgcaat ggtcagaaac aatcccctat gaagtgctct gcgccatcgg ccctcgcatt
      181 cctcgtgtgt atacctctcc ataaaatttc ctgcaatcgc agggtgttca aacaggtcgt
      241 tattctcacc cgcccgaccc tgcgcacgcc gagacgtgcg cttgacccaa gctcacccac
      301 ccacccgcgt ggcgccggga cgccgcgtct gcccaggtct caaaaagcga ctctgattga
      361 gataccgccg gtgtgcagcg tggtctggta aagcccattg accgtgggat tccggttgcc
      421 gacaaccgtc cgatcctcat acagcgccgc ctgatacgac agatccaatc cgatcaactt
      481 gggtttcaga acaccgaccc ccacctcccc gcaggtaaag agaccaagga acttgccctg
      541 ctgtttgcag accagtccga gaccggcccc gacaatgtgt acgtcggccg aaggcacacc
      601 aggatcgaag ccaaaatcgg gcatctgtgt tggctgggta gtataaccgg cccgaagcgc
      661 cacctcccat gaaggcaggg attccagcgt caaccaccga tgctccgttc caatcatcac
      721 cgcataggtg ctccgccaat tcagcggctg aggaattgtg cctccgttgg ccaagtgcac
      781 atcgaggttc cgcacggatt tccagccgac ataatccacg tccacttcca atttccattc
      841 ccgctcacgg ttccgcaccg gccagatggc gacggctcct gtgatcactt gaggcaagac
      901 aaaggtcgct gtcgcatcgg agacttttgc cccgttagcc agtaatgcgc cgtccagatg
      961 cagggtggcc tgactgcgat acacgatacc aatgttggcc agcggcttcc cgtcaggatt
     1021 tctcagcgcg gtgtacagca ggctggcgtt aaatcctgcg gccgtgtccg caccgaacaa
     1081 ttcgatctgc gcgccggcgg gtatcccgcc gccccccggc cacactgatt gccgctcgac
     1141 atggccttcg ccaaacaatc cggaaaacgt gtagatatct gcgcccagtc caatcgagag
     1201 atcgtcggtc agtttgtagg caaccgtcgg cttaatatcc aacaggggca aggtattgaa
     1261 cgtcgtcgcc gtacgaaacg gcccgtcgtt cggccatcga gtgacggacc cgaaaggaac
     1321 cgtcacaccc acgccggccg tgaggtctcc taaggcgcgt atcccgagat ctttcagatt
     1381 tgccgtgagg tagagatggg tgggcggcgg ccacgcgagg ctgccgtcgc gatcgccttt
     1441 cgcgcttacg ccagttggac tcgtgaaggt cgtggttcca ccggagagaa gcgcgccggt
     1501 caagaactcc actccacgca actgcgtcat acccgcgggg ttatagtgga gagctgacgg
     1561 atcgtcggcc tgtgcagaaa aggcattccc cattcccgac gctgccgtcc cctgcccctg
     1621 gatacgaggc acctgggcca acaccggacc atacaggaga gggaacacat acaccgtaag
     1681 cgctgcaacg agagcgatgc gaacgagcat gaaacctcct tcgctcatac catcctcttc
     1741 aatttccgtc caattcttgt cgaggttccc catctgtctg acagacattc tcccaggcag
     1801 aatgtcgagc agtacgcgat gcaagaacaa agctaggtga aaggcggcgc gtcctgacgc
     1861 atctggaccg tgggtacaac gagcgacctt gtcaccttat tctgccgttc gaataaatga
     1921 ttgcattacc tgccgcagtt cctcgacgtc gaacggcttg agcagatagg cctgcgcacc
     1981 aaggctaatg gctcgaatcg cggattcctt cgcgcccgaa gccgtcacca taataattgg
     2041 aatgtgctcg tcgtcttgac ggatctgttt taaaacatcc attccatcga cagatgagat
     2101 accaatgtcg aggatcacgc cggaaaaccg gccggtgccg atcgccttta aggccctcag
     2161 tccgtcggtt tccgtttcca cctcatatcc catcgccatc agacggtcgt ccagcaactg
     2221 gcggatatcc ttgtcgtcat ccacaacgag aattcgaggc gctcgcctgg aaaccgtggg
     2281 ggcaattact tcaacgggaa atctcaacgg caacgtgaaa tacagctccg cgccctttcc
     2341 gtcagggttg cgcgcttcaa tcgtgcctcc atgcagttcg acgagcgtgc gcacgatcga
     2401 caagcccagg ccaagccctt tgacggtcga cctggtttgc gtaatcctga agaacggatc
     2461 aaaaatcttg tccctaaatt ccggtgcaat acctggcccc gtatcgcgga ccgccacgcc
     2521 tgcgtgccgg tgattgtcat tccccaccgt cacggtgatg cgtccctctt cgggcgtaaa
     2581 tttcatcgcg ttctgaacca gattgatcac gatctgaaca agccggtcgc ggtccgccca
     2641 cacgacaagc gggacatctg gatacgtggc ctcgagcgtt tgccgtttcg cctggaccaa
     2701 cgggcgaagc tgctccacca catcggccac gcactggccg agatccagtt cgacaggagc
     2761 cagttcaagc ttgcccgtct gaatcctcgt ccgatccaac aggtcttcga tcatccggat
     2821 cagacgttcg gaattctcga gcatgcggga catgtatcgt tgctgtttct cattgatcga
     2881 tcctgtgaga ccgtccagca tgttctgaag gaaacccttg atcgaggtca tcggcgtttt
     2941 cagctcatgc gatacatgcg agagaaattg cgaccgcagg cggtcggctt gctcgagttc
     3001 cctggtccgt tcctgcactt tggcttccaa tcccacgttg agctcttcga tctgctggta
     3061 ggccgacgcg ttatcaagcg caatggcgac ttgatgggcg aaggtcacca tcaattcgag
     3121 atcgtcctgc gtgagcccat gttcctgcat ccgatccacg atcatgctgc ccaagatccg
     3181 atcctttgtt ttcaggggca ccgccaacag cgactttgag ccggtcaact ggagcagacg
     3241 tcggttgtgg ggatgcatgc gggccacata gggctgaaca tcgccgatca agaggggttt
     3301 gccttgtaag agtacgacgc cttcgagcga ctccggatcg tcaaccggcg tctcgtgcat
     3361 tctcgcaagc tcttggacct ctcgtgaaac accagccacc cgcgcatcca tcgtcattcg
     3421 ccggacggga tcgtagaacg agatcatcgc ccggtcgaaa tgcagatcat ggattaacgt
     3481 ttccaacacc ttttccagaa gggcctcgcg gtcgagcgtc gagctgaaca gcaagccggc
     3541 ctgatgcaag gcggtgagat gattcaccgt tcggcgcagt tcgaccctcg tgtgttcctg
     3601 ttccagatag gcttcgcgca actcttcgtg acgggtctcg acgaagttga tctgttcctg
     3661 gatcaaggcc tcccgccgtt gactctcctt tctcaagcgc cagttgatcc aggttccggc
     3721 aaccagcaca gggatcagtc cagccagcag gacttcgcct agactcatgg aagggtagac
     3781 gagacgcacg atgcctgagg tgagcgcggc tcccgatagg gcccagacat acccggcgat
     3841 atctgtcttc gatttgctta ccggtggtcg ggaccaggct tgcttctgag gcgacgagtc
     3901 accgagggac ggagcgccgt cttgcaatct tataggatga tgatcttgcg ccaccttggc
     3961 atctgcttgc agtcccgcgg agggcacgag tgcatccatt tggacaggcg acaagatcgg
     4021 agacgattcc tccgggatgg ccggcctgga gccaacgccc ctgctccaga gacgacgcgc
     4081 ccacccgtcg gatggctcgg catcccatgt aatgtcccat tggcaccagt cgtcatcgtt
     4141 ggcaatgcag gagcattcgg tcaaggtcgc cggcggcaga ccgtgcaccc gtgctggaac
     4201 ggcagccatg ataccctgcg ccgcctgaca catgacaaag gtgcagcgac ggcgatagga
     4261 tccgaactgg cgcagcgtcc gctcggagaa ccgcatcgcc agcgtggccg agcggttcgt
     4321 gacggcgacg acacggcatt cgatggagcc ggacgcgaac ttgtttccga agtagggaaa
     4381 catgccgtag atctgcgaca gcgagaaagg cctggccaac acctgcatga tcggcgacgc
     4441 cttttccatc cctccacgga agacaaactg aggatcgccg gaaatctgct cgcagaattc
     4501 ataaagatac gcggtgaatt cataggagta gctgttccag gcattcttca agaatgccgg
     4561 tgtcacgtga tacacgggat tcttgatccg cttattgagt gacgaacaca actcctgcac
     4621 ggcctgcagg cctgcggctt ccccgtggcg gcgcgtcatg gagctttcga ggtagaggac
     4681 ggtcgcccgg atactcattc ctgtcaaatc acggatcttc ttgccctgtt cgtccacccc
     4741 gaacgggcga aattccatgt attctcgctc gagaatggtt tgatacttcg gatacaaaac
     4801 tattgtgggt ggaccaggac gaagatcttg cgccgcaccg ttgtgcatgc ctgagactcc
     4861 gtgttgtgtg aggcgggaga ggcgtagatg atggcgcccg tgaatggcgc caccggtatt
     4921 cgggcccgcc tattgcctga ttgaccgctt caacgtcttt tccacgctag cgaccttcga
     4981 gaccaggcgg cccgaatggc cctttcgata atccaccttg atgctgcagg aaatgcgatt
     5041 gcaatccttc ttcatccgtt cgtagcactt ccggacgacg gcgaagacct cgtcccactc
     5101 gccttcgagg accgtcccca tgggattcaa ccgataggcc acgccgctct tatcgatgat
     5161 atccagcgag cgcgagacat acttgctgac gctttctcct ttgcctaacg gggacatact
     5221 gaattccagc aagaccattg cgatcctttc ttctctttgt gacttcacca ctcaggtgag
     5281 cggttaggac tttcccttcc cgcccgcttg ggcagttgct cgtctaactt cttacccacc
     5341 cgccctgccc gcgctgagac gcgggcttgt cccatgctca cccacccact agacgatcat
     5401 ttgttacccg acccgcccac cgattgcttg gcaatcgttt acccggtacg cgccttcgtc
     5461 ttgtcccgct cttcgagcca gactttcgga tactgcacct tgccgtgcag ccggaacccg
     5521 tccaacgctt cgcgcaacat cgtgcggcgc ttctccacat cgggctcatt gctcttcgct
     5581 tgttcgcgca actggccgag ccactccaca aacgcgacaa actccgcatc gaggatgcgc
     5641 tcgagatctt ccttcatgaa tcccgacagg gccggtgcca tcccgctggt gctgatcgcg
     5701 acgcgcgcat ggcccgcttc gacgaccgcg g
//
LOCUS       DASTQY010000380         3367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_682559, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000380 DASTQY010000000
VERSION     DASTQY010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682559"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="VFR82_12320"
     CDS             complement(<1..1475)
                     /locus_tag="VFR82_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEU4502427.1"
                     /translation="MKGKKTQITAEHERLSSDGRVWKRWGPYLSERQWGTVREDYSEG
                     GDAWNYFTHDHARSRAYRWGEDGLAGISDEQQRLCFALALWNGKDPILKERLFGLTNS
                     EGNHGEDVKEYYFYLDSTPTHSYMKFLYKYPQAAYPYDDLVRVNRQRSREMPEYELLD
                     TGIFDDDRYFDVLVEYAKATPEDILIQITATNRGPDEAEIQILPTLWYRNDWAAWQAK
                     PAEKPVLRQVKGPSGTSVAAAKHSIFGEYLLYCDGSEVPLLFTDNETNNARLFPEYVN
                     ASPYPKDGINDYVVNGKQKAVNPDRTGTKVSAQYRFMIGPGRTATAKLRLVRQQAETT
                     ATNPTKPTAKTKAASPFGQGFDSTMKERLREADEFYRVVTPPSVTPDEASVMRQALAG
                     MLWSKQYYYLDADQWLDEHRAHPLHSGSREFRNRDWFHMVNSDVISMPDKWEYPWYAA
                     WDLAFHTLPISIVDPDFAKQQMQLMLRGYYQHPSGQIPAYE"
     gene            complement(1520..2335)
                     /gene="ppk2"
                     /locus_tag="VFR82_12325"
     CDS             complement(1520..2335)
                     /gene="ppk2"
                     /locus_tag="VFR82_12325"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEU4502428.1"
                     /translation="MAKDSEEEKNGNSKLKRKEYEKELRRLQVQLCHLQAWVKETGAR
                     IILIFEGRDGAGKGGTIRAITERVSPRVFRVVALPAPSDREKTQMYMQRYMAHFPAAG
                     EITIFDRSWYNRAGVEHVMGFCTKEQYKAFLEHAPVLEGYITKAGIILIKYWLEVNNE
                     EQERRFQARLDDPLRQWKLSPIDLPSRENWYEYSRARDRMLDATDSKHAPWYLVRSDN
                     KRRARLNCIKHLLSLIPYKKMPSEKVKLPKRSNKHEYDDQATLKGRKFIPEAY"
     gene            complement(2396..>3367)
                     /locus_tag="VFR82_12330"
     CDS             complement(2396..>3367)
                     /locus_tag="VFR82_12330"
                     /inference="COORDINATES: protein motif:HMM:NF044721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502429.1"
                     /translation="AGTKLIAEAWDAAGLYQVGSFVGDSWKEWNGRFRDDARSFFRGD
                     EGSLARFADRFVGSPSLYEHKQREPEQSVNFVTCHDGFTLNDLVSYNDKHNDANDEGN
                     RDGADDNRSWNCGVEGPTDDPEIERLRNRQVKNFFAATLLSIGTPMILMGDEVRRTQH
                     GNNNAYCHDSELNWFDWSLVAKHADVHRFVTLLNERRLMRNMEHERQRMTLAQLIRQA
                     NKSWHGVRVGQPDWSPRSHCMVLNVELKRERRLMHLIMNAYWEPLDVELPGEERQSRW
                     RRWIDTALDSPQDIVPWQSAPSITGATYRIQPRSVVLMFADSEAAST"
BASE COUNT          642 a          991 c          897 g          837 t
ORIGIN      
        1 cattcatagg ccggaatctg accgctggga tgctgatagt acccgcgaag catcaactgc
       61 atctgttgct tggcaaagtc gggatccaca atggagatcg gcagagtatg aaaggcgagg
      121 tcccaagctg cgtaccaggg atactcccat ttatccggca tcgaaatgac atcgctgttg
      181 accatgtgaa accagtcgcg attccggaac tcgcggctgc cgctgtggag tggatgcgcg
      241 cgatgctcgt ccaaccattg atcggcgtcg aggtagtaat actgcttact ccacagcatg
      301 ccggccaaag cctgccgcat gacgctcgcc tcatcgggag taaccgacgg cggggtcacc
      361 acgcggtaaa attcatccgc ttcacggagg cgctccttca tggtcgaatc gaagccttgg
      421 ccgaatggcg acgctgcttt ggtttttgcc gtcggtttgg tcggatttgt ggcggtggtc
      481 tctgcctgtt gccgaaccag ccgcaacttc gcggttgcgg tccgtcccgg gccaatcata
      541 aaccggtact gcgccgaaac cttggtaccg gtgcgatcgg gattgaccgc tttttgcttt
      601 ccattgacga cataatcgtt gatgccgtcc ttcgggtacg gactggcgtt cacatactca
      661 gggaacagcc gtgcgttgtt ggtttcgttg tcggtaaaga gcagcggtac ctcactgccg
      721 tcgcaataga gcaagtattc gccgaagata gaatgtttgg ctgccgccac gctcgtcccg
      781 cttggtccct tcacttgtct gaggactggt ttttcggcag gcttggcttg ccaggcagcc
      841 caatcgttgc ggtaccataa ggtgggcagg atctgtattt cggcttcatc cggcccccga
      901 ttggtcgccg tgatctggat caagatgtct tccggtgtgg ccttcgcgta ttcgaccaag
      961 acgtcaaagt agcgatcatc atcgaaaatc cccgtatcga gcaattcgta ttcaggcatt
     1021 tcccggcttc gctgcctgtt caccctcacc aaatcgtcgt agggataggc cgcctgagga
     1081 tacttataca gaaacttcat gtaggaatgg gtcggcgtac tgtcgagata aaagtagtat
     1141 tctttgacat cttccccgtg gttgccttcg ctgttggtca gaccgaacag gcgctccttg
     1201 agaatgggat ctttcccatt ccagagagcc agcgcaaagc agagccgctg ctgctcgtcg
     1261 gagatcccgg cgagtccgtc ctcaccccac cgataggccc tggaacgggc atggtcatgc
     1321 gtaaaataat tccacgcgtc cccgccttcg ctgtagtcct cgcggaccgt tccccactgg
     1381 cgctcgctca gatacggccc ccagcgtttc cagacccgtc catcgctcga tagtcgttcg
     1441 tgctcagcag tgatctgcgt tttcttgcct ttcatgatct gcccccttcc tctcgtctcc
     1501 cgcctcggcc tatccctctt cagtaggctt cgggaatgaa ctttctgccc ttcagcgtcg
     1561 cctggtcgtc gtattcatgt ttattggacc gcttcggcaa cttcaccttt tcggagggca
     1621 ttttcttgta aggaatcaga ctcagtagat gcttgataca attcaaccgg gcccgccgtt
     1681 tgttatccga ccgtacgaga taccacggtg catgcttgga atctgtggcg tcgagcatcc
     1741 ggtctcgcgc gcgggagtat tcgtaccaat tctcccgcga ggggagatct ataggactga
     1801 gcttccactg gcgtaacgga tcatcgagac gggcctggaa gcgccgctcc tgctcctcat
     1861 tgttaacctc aagccagtac ttgatcagga tgattccggc tttggtaatg tagccctcca
     1921 gcacgggcgc atgttcgaga aaggctttgt actgctcttt ggtgcaaaaa cccatcacat
     1981 gttcgacacc agcccggttg taccaacttc tgtcgaatat ggtaatttct ccggccgccg
     2041 gaaagtgtgc catatagcgc tgcatgtaca tttgggtctt ctcgcggtca gagggtgcgg
     2101 gcaaggccac cacccggaat acgcgtggac tgacgcgttc cgtgatcgcg cggatcgtac
     2161 caccttttcc ggcaccgtcg cgaccttcga agatgagaat gattcgcgcc cctgtctcct
     2221 tgacccaggc ctgaagatgg cacagttgga cctgaagccg gcgcaattcc ttttcgtact
     2281 ctttgcgctt gagtttggaa ttgccgttct tttcctcctc gctgtctttt gccattggct
     2341 cactcctgaa atagctcagt acaccgccgt tttccaaacg ccagctctct gacgattacg
     2401 tcgaggctgc ctccgaatct gcaaacatca agacgacgga acggggctgt atccgatagg
     2461 tcgctccggt aatcgacggc gcgctctgcc atggcacaat gtcctggggt gaatcgaggg
     2521 cggtatcgat ccatcggcgc catcgggatt gcctctcttc acccggcaac tcaacgtcca
     2581 gtggctccca gtacgcgttc atgatcagat gcatcagccg ccgctcccgt ttcagttcca
     2641 cgttcaatac catacagtgg gaacgggggc tccaatccgg ctgtcctact ctcacgccat
     2701 gccaggattt attggcttgc cggatcagtt gggcgagcgt cattcgctga cgttcgtgct
     2761 ccatatttct catgagccgc cgctcattga gcagggtcac gaagcgatgg acatcggcat
     2821 gtttggccac caagctccag tcaaaccagt tgagttcgct gtcatgacaa taggcgttat
     2881 tattgccgtg ctgtgtccgc cgcacttcgt cgcccatcag aatcatgggg gttccaattg
     2941 agagcagggt ggcggcgaag aaatttttca cctgtcggtt gcgcagtctt tcaatctccg
     3001 gatcatcggt cggtccttcc acgccgcaat tccagcttcg gttgtcgtcg gcgccatccc
     3061 ggttgccttc atcgtttgcg tcgttgtgct tgtcgttata agacacgagg tcattcaagg
     3121 tgaagccatc gtgacaggtc acgaagttca cgctctgttc cggttcgcgc tgtttatgct
     3181 catagagcga cgggctgccg acaaaacggt cggcgaagcg cgccaaagac ccttcatcgc
     3241 cgcggaagaa gctcctggca tcatcgcgaa atcgcccgtt ccattctttc cagctgtctc
     3301 cgacaaaact tcctacctga tagagtcccg ccgcatccca tgcctcggcg atgagcttcg
     3361 tgcccgc
//
LOCUS       DASTQY010000381         3845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1113275, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000381 DASTQY010000000
VERSION     DASTQY010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113275"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFR82_12335"
     CDS             complement(<1..266)
                     /locus_tag="VFR82_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502430.1"
                     /translation="MTPPVPPRIYCITATEAPTVAVYRRGPTNWSHVGQWDLAGLRYE
                     PGAWLGGRLFPRRCDLSPDGRFLSYFAHKPSATWEFGEAYVAVS"
     gene            complement(263..661)
                     /locus_tag="VFR82_12340"
     CDS             complement(263..661)
                     /locus_tag="VFR82_12340"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEU4502431.1"
                     /translation="MDVYWEDAVRRGDANIMRDLLSRGIDPNARDRHGQTGLMLAAYA
                     GHLAAVQLLVDHGVDLNVTAKFGLSAVMLAVTAGHKEIAQALARAGADLTLRGTGAPG
                     FAGKTAADLAGARGWSDAAIELAPDTQRMK"
     gene            940..1794
                     /locus_tag="VFR82_12345"
     CDS             940..1794
                     /locus_tag="VFR82_12345"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4502432.1"
                     /translation="MANPRPEELSERRKRLDDLGARYKIASDVRIESVEANGVSAEWS
                     STPHAEPTCVILFLHGGGYVSGSLTSHRPLTTEIGRVCRARTLALQYRLAPEHPFPAA
                     IEDAVAGYRFLLSCGIEPRHIAVAGDSAGGALTLALMVSARDAGLPQPACGWCISPWT
                     DLECLGASMTSKAAVDPLIQKSYLKELAKAYLNGADPRSALAAPLYANLKGLAPLLIQ
                     VGSAETLLDDAVRLASVAGAADLAVTLEIWPDMIHAWPLFHQQLTAGRHAIARAGAFI
                     RSRMSMPF"
     gene            2103..2570
                     /locus_tag="VFR82_12350"
     CDS             2103..2570
                     /locus_tag="VFR82_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502433.1"
                     /translation="MLYDVLHVTERWGPLQVVELSITLLYCIDFFYLQSDLGSDQLPQ
                     GNWRDTILDAGIAVVFGAAYWQASDQKLFTCYALLAVAGALIVAYHFTPNRRVLWAIL
                     PHSVLTILFVSLAWLGRGLDHVTWTFAVYSWFPTIWYGTYVFWMANRVLPTGR"
     gene            complement(3066..>3845)
                     /locus_tag="VFR82_12355"
     CDS             complement(3066..>3845)
                     /locus_tag="VFR82_12355"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4502434.1"
                     /translation="INGIGLHYQESGSRDQPTVVFAHSLMWGANAFTELLTEIARDAR
                     LVTVDLHGHGLSGYREGMTLEEMAEDCYGLWKELMVGKVTWLGCGIGGMIGMRLALAH
                     PDALDSLVLIATSARPDPSTTKASALHLWKMFRDGHRQDIADSAMKLFFAPRTYQARP
                     ELIANCRNELVSTIDARGMFAAALAAFNRSDLSSELHRIKARTLVLAGREDPAATPAQ
                     ADFIASQIPGAEFKIVDEASQLAALEKPSEVARLVREFLHE"
BASE COUNT          802 a         1151 c         1030 g          862 t
ORIGIN      
        1 ttggagaccg cgacgtaagc ctctccaaat tcccaggtcg cactgggttt atgcgcaaaa
       61 taggaaagaa atcgcccgtc cggagagaga tcgcaacggc gcgggaagag gcgaccgccg
      121 agccaggcac caggttcgta acggagaccc gcaagatccc actgtccgac atgcgaccag
      181 ttcgtcggcc cccgtcggta gacggcgacg gtaggtgcct ctgtcgccgt gatgcaatag
      241 attcgaggag gcacaggagg agtcacttca tcctctgtgt atcaggagcg agttcaatgg
      301 cggcatcact ccagcctcgc gcgccggcca agtcggctgc agtctttccg gcgaagccag
      361 gcgcgcccgt gccccgcagc gtcaaatccg caccggcgcg agccagtgcc tgtgcgatct
      421 ccttatggcc ggcagtcacg gccagcatca cggcgcttag accaaatttt gctgtgacgt
      481 tgagatcgac gccgtgatcg accaagagtt gaaccgcggc cagatggccc gcgtaagcgg
      541 ccagcattaa acccgtttgg ccatgccgat cgcgcgcatt gggatcgatg ccacgagaaa
      601 gcaaatcccg catgatattg gcatcacccc gccgaacggc atcctcccaa tagacatcca
      661 ttcaaccgac tcccggcgtg caaatactct tgttttggcg agcagaaagc atacgcggaa
      721 cgaatcgata aatcgagatt gaccatcacg accaatggac gagtcgaaga cttcaagacg
      781 agatcgcggc gcatggcggg gtttcgcaat tcccttttcc ccacactatc cggccaaccg
      841 ccacagtgac ggatagtagc gtctttcaat ggttgggcga ttccctcata ctccccggtc
      901 atggccgacc aagaaatcga tgtcatccgt gcaatcctga tggccaaccc ccgaccggag
      961 gaactcagcg aacggcgcaa gcgcctagat gacttaggag ctcgctacaa aatcgcctct
     1021 gacgtgcgca tcgagtctgt cgaggcgaac ggtgttagtg ctgaatggtc ttcgacgcca
     1081 catgcagagc ctacgtgcgt aatccttttc ctccatggtg gtggttacgt gtctggttcg
     1141 ctcactagtc atcggccgtt gacgacggag atcggacgag tgtgccgggc tcgcacgctg
     1201 gccctgcagt accgactggc acccgagcat ccgttccctg cggcaatcga agacgcggta
     1261 gccggttatc gcttcttgct gtcatgcggc attgaacccc gccatattgc ggtggccggt
     1321 gatagtgccg gaggcgcact gacgctggct ctgatggtct ccgcccgcga tgctggccta
     1381 ccgcagccgg cttgtggttg gtgcatctct ccctggacgg acttggagtg ccttggcgca
     1441 agcatgacca gcaaggctgc ggttgacccg ttgatccaga aatcgtatct caaggagttg
     1501 gctaaagcct atctcaatgg cgctgatcca cgctcagcct tggccgcgcc actgtacgca
     1561 aatctcaagg gacttgcgcc tctcctgatt caggtgggct cggcggaaac actcctcgac
     1621 gatgcagttc gtctcgccag tgtcgcgggg gccgcggact tggcggtgac attggaaata
     1681 tggcccgaca tgatccacgc atggccgctg tttcaccagc aactcacagc cgggcggcat
     1741 gccatcgcgc gcgccggagc gttcatccgc tccaggatgt caatgccatt ctgactgaga
     1801 agtctcttct cgcggtacgt cgtggaggct acccctttcc aagaaccccg gtgctctgtt
     1861 aattttgaat tccgctgatg aacagtcagg taagatgcac tgtttcttga tgataaaacg
     1921 agcccacagt gaaagctgtg ccgtacaccg gtgtggtgcc cgcgcatgaa tgtgcagact
     1981 aatactgtgc tggagccaac agccgctccg ctgctcggtt ccactcgata gtgcgattca
     2041 aagaactctc agcctggcat cgtttcacgt acacgcagct atatccggct gtgctgggat
     2101 cgatgcttta tgatgtcctc catgtcacag aacgatgggg accgctccag gtcgttgaac
     2161 tatccatcac acttctctat tgcatcgact tcttttactt gcagagcgat ctcggctccg
     2221 accaactccc ccaaggcaat tggcgggata cgatattgga cgcaggcatt gccgtggtat
     2281 tcggtgcagc gtattggcag gcgtcggatc aaaagttatt cacgtgctat gccttgctcg
     2341 cagtggccgg cgccctgatc gtcgcctatc acttcacgcc aaaccgccga gtcctgtggg
     2401 caattcttcc gcattcggta ctgacgattt tattcgtgag cctggcctgg cttggtcgcg
     2461 ggttggacca tgtgacgtgg acctttgccg tttactcctg gtttccgaca atctggtatg
     2521 gcacatacgt gttctggatg gcaaaccgtg tgttaccgac cgggagataa cgtggagctg
     2581 cgattggctt ctttgttctg aaacctgaaa gtccgcactg ccatccacgc tatttggatt
     2641 tggctccgaa gctgaatcag tattcaaagg cgacggtata cagagctgga gtcatttcaa
     2701 tccctaactg atccagcttc cccttcatgg gagccgcact ttgctcatag gccttccaat
     2761 acagatcttc gttctcccag agcgcaatat tgatgaagtt gtaacgactc tccggtttga
     2821 gacttttatg aaactttccg ctgatgaatc ccgggcgctt tgtgatgttc gcctttacgt
     2881 catgccacca cttgacgaat catcttcctt cccacttggg accgaaaaca cattaatcaa
     2941 tgtcacggcc atagcagacc tccctcctca tttcacactg tgcctctaac ggtcaacgta
     3001 gtggtcgaac acgcctaatc ttagctcgat cgattccctc cttagcgacc gcttgcttca
     3061 ctcattcatt catgcaggaa ctcccgaacc agtcgggcga cctccgaggg cttttcaagc
     3121 gcagcgagct ggctggcctc gtcaacaatt ttgaactctg cacccggaat ttgtgaggcg
     3181 ataaagtccg cttgtgccgg cgtggctgcc ggatcttccc gaccggcaag gacaagcgtc
     3241 cgagctttaa tcctatggag ctcgctgctc aagtctgagc ggttgaaagc ggcaagcgcc
     3301 gcggcaaaca tgccgcgggc atctatcgta ctcacgagct catttctgca gttcgcaatc
     3361 agttcaggcc gggcttgata cgtccggggc gcgaagaaca atttcattgc cgaatcggca
     3421 atgtcctgtc ggtgtccgtc ccgaaacatc ttccacagat ggagcgccga tgctttcgtt
     3481 gttgagggat caggtcgcgc actcgtcgcg atcagcacga gcgaatcaag ggcgtccgga
     3541 tgtgcaagcg ccagacgcat accaatcatt ccaccgatcc cacatcccaa ccaggtaacc
     3601 ttcccgacca tcagttcttt ccacagccca tagcaatctt ccgccatctc ttcgagtgtc
     3661 ataccctcgc ggtacccact taggccatgg ccatgaagat cgaccgtcac gaggcgggca
     3721 tctctcgcaa tttccgtcag cagctcggtg aaggcgtttg cgccccacat gagcgaatgg
     3781 gcgaacacga ccgtcggttg gtcccggctc cccgactctt gataatgaag cccgatcccg
     3841 ttgat
//
LOCUS       DASTQY010000382         4971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_682563, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000382 DASTQY010000000
VERSION     DASTQY010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682563"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VFR82_12360"
     CDS             complement(<1..399)
                     /locus_tag="VFR82_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HEU4502435.1"
                     /translation="MRKSTKRRSSRLWPILCTLVAIPCAALAFLWLVTLPDVSSLRSV
                     NPTTTALIEARQIEAEAQGMRIGRRWIWVPLSRISPYLRRAVVAAEDASFFTHEGFDW
                     EGMKDAAIYDLEQGELKRGGSTITQQLAKNL"
     gene            434..1036
                     /gene="yihA"
                     /locus_tag="VFR82_12365"
     CDS             434..1036
                     /gene="yihA"
                     /locus_tag="VFR82_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEU4502436.1"
                     /translation="MKILKAEFIRSCVGPAQFPTDSTSEIAFVGRSNVGKSSLINSLL
                     QRKGLAKVSRTPGKTRAINLFTVVTDDPQLSRFVLADLPGYGYAKVSKTERAQWAPLI
                     EAYLGRDGQVCTVVMLVESRVLGAQDAETLRWLRSIGYEPIVVATKADKLKAGERVAA
                     LRRVQEGLGLAGSPTFMAYSSVTGEGRERLWSALKERCQT"
     gene            complement(960..1976)
                     /locus_tag="VFR82_12370"
     CDS             complement(960..1976)
                     /locus_tag="VFR82_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEU4502437.1"
                     /translation="MNTSSCTGTWLRLGISALLSLSCFGATSFAAKVEDRIAAIVNSD
                     LIMLSEVRRELAPERERLEKHYRGEELSRRLQMAEAMALTTMIERRLQLQEAKARSVE
                     VGDQEVKQAVKQLIQQGEKIDEKDPVSTNTVRDQLTLLKVVDREVRSGVMVADPEMKR
                     YYQEHRDRFALPEEYTLSQIFIKPRSPDDVADARAKGREVTALLKQGESFEDLALRYS
                     DGPTASRGGRLGLVRQGELLPAIERGVSNLVPGGISDIIESPEGLHIVRLDDKKPKQF
                     RPFEEVRQEIQALVFQQKSEDMFQSWLADLKNKAYIEIKFDSAPLRQTTSVPVPPPSP
                     TSKP"
     gene            complement(2151..3053)
                     /locus_tag="VFR82_12375"
     CDS             complement(2151..3053)
                     /locus_tag="VFR82_12375"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU4502438.1"
                     /translation="MHQRPTEYATASAVPHMCLMMMAVVGLLLPLVGCTPPQAEERVV
                     AFVNGKPITQVELDHEWADLPDATKARYEKEGGRQVFLKELVDHEMLLQEARRQGLDQ
                     DDAIRDRVRRYKEKLLIDELLKDRMKTTVELTKEELDRYYEQHASELLTPLKVRVAQM
                     LLPNYPAAKDLETQVNRGGDFGKFAQRYSIDYKTKAKGGDLGPYRKGLVIPEVDDAIR
                     TLKPGMISAPIKTEAGYYLVMITALEPEIIQADLAKRERLRQELLYLKRKQYFDSVIA
                     EIKGKAVVRLADGSRFSTDGLTKR"
     gene            complement(3076..>4971)
                     /gene="mfd"
                     /locus_tag="VFR82_12380"
     CDS             complement(3076..>4971)
                     /gene="mfd"
                     /locus_tag="VFR82_12380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU4502439.1"
                     /translation="PLDRLNQIQRYSGAEGHVPRLDRLGGTSWAKTTARVKKDIEEMA
                     QELVDLYANRELAKRHSYGHDSTLYREFEAAFEYEETPDQLKAIEDITRDMETSKPMD
                     RLVCGDVGYGKTEVAMRAAFKAVEDNRQVAVLVPTTLLAHQHYDNFAERFAPFPTRVA
                     LLSRFQSPREAKTILKEVEAGIIDVVIGTHRLLQKDVRFRDLGLVIIDEEQWFGVKHK
                     ERLKQLRAQVDVLTLTATPIPRTLQMAMASVRDLSIIDTPPAGRLSIRTQVVRSSDKL
                     IREAILRELGRGGQIYFVHNRVETMERTGAWLQQIVPEARVVMAHGQMDAKPLEAVML
                     KFFRREADVLVASAIIQSGIDVPHANTIIVNRADTFGLAQLYQLRGRVGRSGDQAYAY
                     FLVPDEGRLTDDAQKRLTAIQQFTELGSGFRIAAADLEIRGAGNLLGKQQSGHIAAIG
                     LDLYMQMVEQAVQRLKGQIVEEEPDPTLRLNVSAFIPEDYVADPHHRLSLYKRLSSST
                     QVGDLALLHGEIQDRFGPPPEPVERLFEVMQIRLQAKALRLVSIELKAGAVAVTLDGK
                     SRVPQTAIQHLMDRYKTRIRFLSPLAFELQMPHQDWASIFPELTATLQSLGVCDTNST
                     ATSPSPT"
BASE COUNT          925 a         1462 c         1488 g         1096 t
ORIGIN      
        1 cagattcttt gcgagctgct gggtgatggt gctgccgcct cgcttgagct ccccctgttc
       61 gaggtcgtag atcgccgcgt ccttcatgcc ttcccagtcg aatccttcat gcgtaaagaa
      121 cgaggcgtct tccgctgcca cgaccgcacg gcggaggtac ggcgagatgc gggacagcgg
      181 cacccaaatc caacgccggc caattcgcat gccttgtgcc tcggcctcga tctgacgcgc
      241 ttctatcaag gcggtggttg tcgggttgac cgatcgcagc gaggacacgt cgggaagcgt
      301 caccagccag aggaacgcca aggccgcgca tggaatggcc accagcgtgc agagtatggg
      361 ccacaggcga ctgctgcgcc gtttggttga cttccgcata cgggaaccgt atgacatgtg
      421 cacaggacct ttcatgaaaa ttctgaaggc cgagtttatc agaagttgcg tcgggccagc
      481 acaatttccg acggattcca catccgagat tgcctttgtc ggacgctcca acgtgggcaa
      541 gtcctcgctc atcaattcct tgctgcagcg aaaagggctg gcgaaggtga gccgcacgcc
      601 cggcaagacg cgggcaatca acctgttcac ggtcgttacc gacgatccgc aattgtcgcg
      661 gtttgtgctg gccgaccttc ccggctacgg ctacgccaag gtgtcgaaaa cggaacgggc
      721 ccaatgggcg ccgttgatcg aggcgtacct gggacgggac gggcaggtct gcacggtcgt
      781 catgctggtg gaaagccgcg tcctcggggc gcaggatgcg gagacgcttc gatggctcag
      841 gtcgatcggc tatgagccga tcgtcgtcgc gactaaggcc gacaagttga aagccggcga
      901 gcgtgtggcg gcgctccggc gtgtccagga aggacttggt ctggcaggaa gcccgacgtt
      961 catggcttac tcgtcggtga cgggggaggg acgggaacgc ttgtggtctg ccttaaagga
     1021 gcgctgtcaa acttgatttc gatgtaggcc ttgtttttca agtcggccag ccacgattga
     1081 aacatgtcct cgcttttttg ctgaaagacg agcgcttgaa tttcctgcct tacttcctca
     1141 aacggcctga actgcttcgg tttcttatcg tccaaccgca cgatgtgcaa gccctccggg
     1201 ctttcaatga tgtccgaaat gccgccggga acgagattgg aaactcctcg ctcgatcgcg
     1261 ggcagcaatt cgccttggcg gaccagcccc aatcgacccc ctcgtgacgc cgtcggtccg
     1321 tccgaatagc gaagcgccag atcctcaaac gactcccctt gtttaagcag agcggtcacc
     1381 tcgcgaccct tcgctctcgc gtctgcgacg tcgtcgggag agcgcggttt gatgaaaatc
     1441 tggctcaacg tatattcttc gggaagcgca aaccggtcac ggtgctcctg atagtaccgt
     1501 ttcatttccg ggtcggcgac catcacgccg ctccggactt cccgatcgac gaccttgagc
     1561 agcgtcaact gatcccggac ggtattggta ctgaccgggt ctttctcgtc gattttttcg
     1621 ccctgttgaa tcaactgctt gaccgcctgt ttgacctcct ggtctcccac ctccacacta
     1681 cgggcttttg cctcctggag ctgcagccgt cgttcgatca tcgtggtcaa ggccatcgct
     1741 tctgccattt ggagtctgcg ggacaattcc tctccccgat agtgtttctc gagccgttcc
     1801 cgctccggcg ccagctcgcg cctgacttcg gacagcatga tcaagtccga gttgacgatc
     1861 gccgcgatgc ggtcttcgac tttggctgcg aaagatgtcg ctccaaaaca gcttaggctc
     1921 agcagcgctg aaatcccaag tcggagccaa gtgcctgtgc aagacgaggt gttcatggtc
     1981 tgtgggcgcg aggggttgac gctgtcgcgc gatgtggagg gtgattgtac aagattcagc
     2041 gttgcatgcg gaagagaagg ataggcgacg cgagaacaaa gcgtggtgaa ggacgcgccc
     2101 ctgcgcgtcg ggcaagggtg ggtgcgatga gggacttgtt cagcagcctg ctagcgcttg
     2161 gtcagaccgt ccgtgctgaa gcgtgaccca tcggccagtc gcacgacagc cttgcctttg
     2221 atttcagcga tcacactgtc gaaatactgc ttgcgtttga gatagagcag ctcctgtcgg
     2281 agccgttcgc gtttcgcgag gtcggcctgg atgatttccg gctcgagcgc cgtgatcatg
     2341 acgagatagt agccggcctc ggtcttgatg ggtgcgctga tcatgccggg cttgagcgtc
     2401 cggatggcgt catccacttc ggggatgacc aggcccttgc gatacggccc gaggtccccc
     2461 cctttggctt tcgtcttgta atcgatcgag tagcgctggg caaacttgcc gaagtcgccc
     2521 ccgcggttga cctgcgtttc cagatccttg gccgcaggat aattgggcag caacatttgc
     2581 gccacccgca ccttgagcgg cgtcaagagc tcactggcat gctgctcgta atagcggtcg
     2641 agttcctctt tcgtcagctc gaccgtcgtc ttcatccggt ctttcaacaa ctcatcgatc
     2701 agcagttttt ccttgtagcg tcgcacccga tctcggatcg catcatcctg atcgagccct
     2761 tgtctgcgag cttcctgcaa gagcatctcg tggtcgacca gttccttcaa aaagacctgc
     2821 ctgccgcctt ccttctcata gcgcgccttc gtggcgtccg gcaaatctgc ccactcgtgg
     2881 tcgagctcga cttgtgtgat tggtttgccg ttgacgaatg ccaccacacg ttcttcggct
     2941 tgaggcggcg tgcagcccac cagcggcagg agcaggccca cgaccgccat catcataaga
     3001 cacatgtgtg ggacggcgga ggcagtggcg tattcagtcg gtcgttgatg catgcggaag
     3061 agaatccttg tggagttacg tgggtgaagg cgatgtcgct gtgctgttgg tatcacagac
     3121 tcccaggctt tgcaaggttg cggtgagttc ggggaagatg gaagcccagt cctgatgcgg
     3181 catttggagt tcaaacgcga gcggggaaag aaaacggata cgcgtcttgt atcggtccat
     3241 gaggtgctga attgccgtct gcggcacgcg gctcttcccg tcgagcgtga ccgcaacggc
     3301 cccggctttc agttcgatcg acaccagccg caaggccttg gcctgcagcc gaatctgcat
     3361 cacctcaaac aaccgctcga ccggctcagg tggcggaccg aagcgatcct gtatttcacc
     3421 atggagaagc gcaaggtcgc ccacctgggt ggaggatgac agccgtttgt acaaggacag
     3481 gcgatggtga gggtcggcca cgtagtcttc agggataaaa gcggagacgt tgagacggag
     3541 tgtcgggtcc ggttcttctt cgacgatttg tcccttcaac cgctgcacgg cctgctcgac
     3601 catttgcata tagagatcca gtccgatggc cgcgatgtgt ccagactgct gtttgccgag
     3661 cagattgccc gctccgcgaa tctcgagatc ggccgccgca atccggaatc ccgaaccgag
     3721 ctcggtgaac tgttggatgg ccgtcaagcg tttttgcgca tcgtccgtca acctcccttc
     3781 atcgggcacg agaaaatagg cataggcctg atcgccgctg cggccaacac gaccgcgcag
     3841 ctgatacagt tgggccagac cgaacgtgtc ggctcgattg acgatgatgg tgttggcgtg
     3901 gggcacatcg atgcctgatt gaatgatggc cgaggccacc aggacgtctg cctcgcggcg
     3961 gaaaaatttt agcatcacgg cttccagcgg tttggcatcc atctgcccat gcgccatgac
     4021 cacgcgcgct tccggaacga tttgctgaag ccacgcaccg gtccgttcca tcgtctccac
     4081 tcgattatgg acaaagtata tctggccgcc tcgtccgagc tcccggagaa tggcttcccg
     4141 aataagcttg tcgctggagc ggacgacctg cgtacgaatg gacaggcgac cggccggtgg
     4201 cgtgtcgatg atcgagagat cccgcacgct cgccatggcc atttgcaagg tgcggggaat
     4261 cggcgtcgcg gtgagcgtga ggacgtcgac ctgtgcgcgc agctgcttca gccgctcctt
     4321 gtgcttgacc ccgaaccact gttcctcgtc gatgatcacc agaccgagat cgcggaaacg
     4381 tacatccttt tgcagcaggc gatgggtacc aatcacgacg tcgatgattc ccgcctccac
     4441 ctctttgaga atcgtcttgg cttctcttgg cgactgaaag cgtgagagga gcgccacgcg
     4501 ggtcggaaac ggcgcaaacc gttcggcaaa attgtcgtaa tgttgatgcg ccaggagcgt
     4561 ggtggggacc agcaccgcca cctggcgatt gtcttccacc gctttgaatg cggctcgcat
     4621 ggctacctcg gtcttgccgt agcccacatc accgcagacc agccggtcca tgggtttgct
     4681 cgtttccatg tcgcgagtga tatcctcgat cgctttcaat tgatcgggcg tttcttcgta
     4741 ttcgaaggct gcttcgaact cgcgatacaa ggtgctgtcg tgcccatagg agtgccgctt
     4801 ggccagctcg cggttggcat agagatcgac cagttcctgc gccatctcct ctatgtcttt
     4861 cttcacgcgc gccgtcgttt tggcccagct cgtgccaccg agccggtcaa gccggggaac
     4921 gtgtccctcg gcgccgctgt accgttggat ctgattaagc cggtccagcg g
//
LOCUS       DASTQY010000383        20346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_482712, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000383 DASTQY010000000
VERSION     DASTQY010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 20346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20346
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482712"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            452..739
                     /locus_tag="VFR82_12385"
     CDS             452..739
                     /locus_tag="VFR82_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502440.1"
                     /translation="MNYLWTKLAGSAMGAVVFSVALSACRPVPIATVQPKSDPVVDMS
                     AKVQSLQKQIQERDKRIEELEAQLDALKLIDQDREKQRKPLRAPTSVEPIQ"
     gene            879..1667
                     /locus_tag="VFR82_12390"
     CDS             879..1667
                     /locus_tag="VFR82_12390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4502441.1"
                     /translation="MPSWPLPKHDSWSTPFAESLLTHLDLFSGATILDVASGHGIPAF
                     HLAEQVGRTGQVVGVDLNAHQVRSARTIQGQQLPWLHFECLDMRALPSNLPSFDRITG
                     NLSVMFFRPDRFTTLQNLSAHLKSGGQMVLTFPSLGTFDSLWQRVDQEMAARRLSEER
                     RRLEAYVGERPSAEQGREWLHKLRFERIIVSEYPLEVAVGHGRAFLHDPLLRGGFLDD
                     VYECFDDAVLANEIMTAVSEDVASFVPLIALRCVLSGWKPSGRG"
     gene            complement(1701..1955)
                     /locus_tag="VFR82_12395"
     CDS             complement(1701..1955)
                     /locus_tag="VFR82_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502442.1"
                     /translation="MRRIMMGFVWSLLLYFGMLGIGGAVLGSAAGFRETHVDQGYRAG
                     HAAGQEFGNKYREIILLIAVAGAAIGTFTGRLPGTKPKTK"
     gene            complement(2132..2656)
                     /locus_tag="VFR82_12400"
     CDS             complement(2132..2656)
                     /locus_tag="VFR82_12400"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU4502443.1"
                     /translation="MRTWGQVGLAGTWADASIHLYGPHDRSSGTRSFFKEAVLLNGNF
                     KRSLKTEPGLASVIVAVGKDRYGIGYSHIGLETSAVRAVPVNGKSGRTAVAPTAASVM
                     SGEYPLSRPFYLYINQRPDKDWDPEVHEFMKFVNSRDGQEIVARTGLFPLSTSQVIEH
                     HRMLRARVIKGSPP"
     gene            complement(2653..3102)
                     /locus_tag="VFR82_12405"
     CDS             complement(2653..3102)
                     /locus_tag="VFR82_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU4502444.1"
                     /translation="MDAYIAHAQVRGRMTIVGSDATKPLLLKLAMEFRRRQPEATIAV
                     QGPRGSKISPLDAFVSGLSGMRRGDGDPAGHFGSYDVELLASPRALTEQEFERFTSKH
                     GHEPTGFVIAYGALALYVNRENAINSLTLEQIDGNFFCRLISAAWRT"
     gene            3413..3883
                     /locus_tag="VFR82_12410"
     CDS             3413..3883
                     /locus_tag="VFR82_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502445.1"
                     /translation="MRKRMSRQVQEGAGLECNWLNVEELAEVEITSEDSAHPVESAFV
                     AGSTSGWRAAEPGDQTIRLLFNHPQQLKRIWINFVELETERTQEYVLRWSSDNGHSFR
                     EIVRQQWNFSPPHTTNEIEDHRVELPATQILELSIKPDISNGAAVASLACMRLA"
     gene            4231..4755
                     /locus_tag="VFR82_12415"
     CDS             4231..4755
                     /locus_tag="VFR82_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502446.1"
                     /translation="MHVHRIILGSALVLLAGCQSIGGESMPTLTRTGQVKDVVIRDVV
                     EPTSLSVNPGDEVRWINKRQGAVRVIFLNPMTENLNCVRNFGGPLGFGTKRNEYSAKL
                     GSNETASVCFRDSGEVKYVVRAESASDPSGEQNIAGTISIGSEDQASRPVSKPVERNE
                     GLAQSDKDEAASSR"
     gene            5075..6661
                     /locus_tag="VFR82_12420"
     CDS             5075..6661
                     /locus_tag="VFR82_12420"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU4502447.1"
                     /translation="MAQKKSERAGAAQPLSAWKTALRDWQARAVADVLSHPAPDFLAM
                     ATPAAGKTRFALRVAHEYVRTRRAQRLLVVCPTNHLRSQWAQAAGTVGLHLDPALTNE
                     QTCEAPDYHGAVVTYQQVALAPRVFQRGCAGRNTLVVLDELHHAADGRHWGRALRQAF
                     DPAVFRLVLSGTPFRSDNHPIPFVRYEQGESRADFAYGYTDALRDGVCRPILFPSYEG
                     ELSWLSEGREHRATFEDGLTFERQRERLKTALLQESWLGPVVADAHQQLTRLRKQEQP
                     DAAGLIVSMDQDHARSVAALVEKWTGTKAAIAVSDDPAASRTIKTFAGQRKQQWLVAV
                     NMVSEGVDIPRLRVGVYGTNVLTEMYFRQVVGRFVRMQPHVSTPQRAWLYLPKDPVLV
                     RYARQIKAERDHVLEDILPAGQRDLFGRTTVSLKEYTPLHAIPRVDSLIGEDEAQPDG
                     MATATPEPPLHDRKRDLRDLHRVLVGSLARAAGIDHRRLNAELISRTGSRVDQATLEQ
                     LEKRIQLLQRWRDRGYDGKR"
     gene            7285..8301
                     /locus_tag="VFR82_12425"
     CDS             7285..8301
                     /locus_tag="VFR82_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEU4502448.1"
                     /translation="MSQMTAVEIRSPGTPFEVVMREIPAPGPNRVRIRVQACGICHSD
                     MFVKEGYWPGLQYPRVAGHEVAGVVDEVGAGVTAWKKGQRVGVGWHGGHCGQCDPCRR
                     GDFMGCRYFQVTGFQEDGGYAQYMIAKSEAVAAIPDALSPVEAAPILCAGVTTFNSLR
                     HSEAVAGDLVAVQGLGGLGHLGIQFASRMGFHTVAIGRGKDKEPLALKLGAARYLDTG
                     SMDVAKELTSLGGASVILATAPDSKAMSALIDGLGVGGQLLVVGASADPITVTPIQLI
                     GGRRSIQGWPSGTARDSQDTLNFCALTGIRPMVETLPLEQAAAGYERMLSGKARFRVV
                     LTVT"
     gene            complement(8341..9300)
                     /locus_tag="VFR82_12430"
     CDS             complement(8341..9300)
                     /locus_tag="VFR82_12430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEU4502449.1"
                     /translation="MTTAKSSSIRVIKPGWCTTVQDLGRHGYQHYGVSVSGAMDRLGL
                     VIANRLVNNPDVAAALEITIVGPELLFEDAAVLAITGADLSPAIDGHSVPIWTTVAIQ
                     AGSRLTFGRRRTGARSYVAVAGALDVALLWGSRSTHISSCTGGFSGRALLADDLLRVS
                     VDQRERPHIGAMLAPGNRPLYVDSPTLRVISGPQHVNAAALSFLTATPYRLTSQSNRM
                     GYRLEGHRLHEDPTPPRLSDGTAMGALQIPPDGSPILLMADRPTTGGYPKIATVISAD
                     LPQAAQLQPGDTLTFRTTTLGEAELALMNQWRHINEVLPPRRS"
     gene            complement(9287..10057)
                     /locus_tag="VFR82_12435"
     CDS             complement(9287..10057)
                     /locus_tag="VFR82_12435"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEU4502450.1"
                     /translation="MLRRLDLNSDMGERDTADGMALDAALMPLITSVSIACGGHAGSR
                     DLMRRTASVAQQHGVAMGAHPGLPDRETFGRTERPVTANEIRALVSEQVMVCGRVLKT
                     EGFTLRHVKPHGALYTMAARDEEIAEAIVSAVRDVDGALLLYALAGSMLARVGKAAGL
                     EIIQEGFADRAYRADGRLVSRSDSGALIEDTDGVREQLRRLLNETVMTVDRRLIPMSI
                     HSLCLHSDTPQALVLARVIREELQRNGIEVMAASHDDR"
     gene            complement(10041..10733)
                     /gene="pxpB"
                     /locus_tag="VFR82_12440"
     CDS             complement(10041..10733)
                     /gene="pxpB"
                     /locus_tag="VFR82_12440"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEU4502451.1"
                     /translation="MTRNGFAVLPLGDAAVTVEFGCTIEPGLNEQALAFAQAVRIQQW
                     EGVVDIVPAYASVTIHVDPFRLPISTLSARLHDLSSDGKDLLSGQAHRIPVLYGGEWG
                     PDLHDVAAFARQSLAETIRLHHATHYRVYMLGFSPGFPYMGVVPPSIAMPRLPTPRTV
                     VPPGSVGIAESQTGIYPTATPGGWRIIGRTPVSLYRPQSSSPFLFSPGDHVQFYPIDA
                     DEFTRISNAAPT"
     gene            complement(10848..11321)
                     /locus_tag="VFR82_12445"
     CDS             complement(10848..11321)
                     /locus_tag="VFR82_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502452.1"
                     /translation="MDPFRIVPLIKTMWKLSSSLIGVHRERDIAIFHRLDSILDESRV
                     KNILDYSFFTECLRFEERELLCKFGDALQRLENEYLHPVIQVRATALAREMSQLLRTV
                     GATFRSDDGEIYRFRPDPIDPTAYDREWDKLHEQVEITSKAYRTYRQAVKKRLKV"
     gene            11426..11803
                     /locus_tag="VFR82_12450"
     CDS             11426..11803
                     /locus_tag="VFR82_12450"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4502453.1"
                     /translation="MSTVSVRYIIDDVPAAMRFYTTLLGFTVERDASPAFVSVVRDGV
                     RLLLSGDGSSGKRPLTDGRQQVPGGWNRVHLEVTDIAEEVARLRGAGVSFRMDGIVTG
                     PGGAQIILDDPSGNPVELFQPGH"
     gene            12045..12860
                     /gene="modA"
                     /locus_tag="VFR82_12455"
     CDS             12045..12860
                     /gene="modA"
                     /locus_tag="VFR82_12455"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4502454.1"
                     /translation="MDSRTLKMAIAGLVAIVGICEPLQAEPLSVGAPPSLRPAFSEIL
                     PMFEREYGAAVNIVYTPSKTLLRQIEKGASIDVFLSAGIEEAEYLHKKGLTLNGRPRI
                     YAQTSLVLVMSSDSQATHMTLRDALNSRSTRIALGDPQTSSLGDITARALSKTYPTYK
                     RRPNVVYAPHSEDIMDLIRKGKSDVGLVYRVDSINSGYVRISDEDPFGVLVPVQFGQA
                     VASTCRPALRSAADQFSSFLMTPRIQKLLVKYGFDSVPLPVSHIPTERKERAS"
     gene            12857..13453
                     /locus_tag="VFR82_12460"
     CDS             12857..13453
                     /locus_tag="VFR82_12460"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEU4502455.1"
                     /translation="MTRKAVTWITVMTTMAALAAPSIFAIEKVAGPVGVPPEIVANYL
                     RAVIMAHRHFYTIHIANRLRQERIVDVSENWRTTHSLPLPVQLVRETSEIAELTGPNV
                     RYRLISQWPINKANAASTEFERRGLKEVQARPNRPYTSSSTVPTNAEQLFEAVYADVA
                     VTSACVECHNTHPSSPKKDFKVGDVMGGLVISFPLGKQ"
     gene            13628..13891
                     /locus_tag="VFR82_12465"
     CDS             13628..13891
                     /locus_tag="VFR82_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502456.1"
                     /translation="MMLEREAWLYAAAVLGVLASIAVGRTSGKVEEAPVQARLWKFAK
                     SWLLVVALAALSVLHGKWQPMVFFAFVGVMSSALFWWGCRSSK"
     gene            complement(13949..14914)
                     /locus_tag="VFR82_12470"
     CDS             complement(13949..14914)
                     /locus_tag="VFR82_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4502457.1"
                     /translation="MTSRPVRTALLLALGAVIAALGTATYFYHTAIQEARDRVATGSS
                     IAHTSCGPIEYAFAGDGPPVLAVHGAGGGFDQGLDFGAPLAKLGFRVIAMSRFGYLRT
                     PLPPDASPAAQADAHACLLDALEIEKVAIVGGSAGAPSSMQFALRHSARLTALVLVVP
                     ATFVPRPGNAPALHTPPGTQFLFDTALKWDVVFWAVSRFAPGAAIRYILATPPNLVRD
                     AAPEEQARVQRALEHILPIAQRRLGLLNEAAVIPSLQRYELEKIHVPTLTISCADDVF
                     GTYDGARYTAEHIPGARFVGYETGGHLWVGHQQEMTDEIARFLKP"
     gene            complement(14911..15996)
                     /locus_tag="VFR82_12475"
     CDS             complement(14911..15996)
                     /locus_tag="VFR82_12475"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HEU4502458.1"
                     /translation="MKDQHRHTRIIVTHYGGPDALQVLEEDRPEPKEGEVRVRVLAAG
                     VSLPDVMMREGIHPETPTLPFTPGWDLVGVVDRIGDGISMLETGQIVAALPINGAYAE
                     FVCLPQRELVPVPPELDAAEAVSLVLNYVTAYQMLHRSAKVRPGQRVLIHGATGGVGS
                     ALLQLGRLARLQMYGTCSSQGASTVSTLGGIPIDYQHQDFVAEIHRFTSEGVDVVFDG
                     IGGTHIWRSRKALRSGGTVVAYGITSSLYGGRMTSGRSRGRHRFRAIAIFGLYIAAGW
                     ILPGRKRVVPYSVQWLKRLRPVLFRENLIALFELLHQAKIKPLIAKRFPLVQARQAHE
                     FLEKGGVTGKIVLVPNGSAISERGEWT"
     gene            complement(16209..17093)
                     /locus_tag="VFR82_12480"
     CDS             complement(16209..17093)
                     /locus_tag="VFR82_12480"
                     /inference="COORDINATES: protein motif:HMM:NF039293.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502459.1"
                     /translation="MADQDSQLTPGTLRASLLERTATARRRGAIQFLPTTLEVIEQDS
                     IPFQVRVLESPAMNAITEAARSPVNPFPYDQDLFVASISATHIALLNKFSVFEHHLLI
                     ATNAFEDQRCQLTQKDCEALLIGLTEMDALAFYNAGQVAGASKLHKHLQLVPLDAPIA
                     PLPMEPLLRFAQTDRMIGTVPRLPFRHAFAPMDPDWTNPGKDSGASLHACYRKLLHAA
                     GLSVDASSGSHDLTAPYNLLMTRRWMLVAPRSQECFEGISVNALGFAGAFLVKDRSQL
                     ATLRRSGPMTALQHVTAQ"
     gene            complement(17707..18705)
                     /locus_tag="VFR82_12485"
     CDS             complement(17707..18705)
                     /locus_tag="VFR82_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502460.1"
                     /translation="MTRWLAHGSTLFAMMFLSGCVSSPPRMPAEDIGFTRAPQLGGTD
                     ALWIMHHDGSGQSLLNLDAEGNSSLTWSPDGQYIAFESMRDGNLEIYTARMIAGGDHT
                     YSAQDIQRRTTSAGDDSFPAWSQDCAVLAFSSNRINPSYYNIYQLNVSTSTVTSITSG
                     NFEDLSPSWSPDGTKIAFTRNVVNASREIFVHNLNSGLDVRLTNNTVNDTDPSWSPSG
                     RIIFARQSEDGSRAALFEMDAVDSDGDGAGDHIQPVTTPNANEYDQKPEYSHSGKEIL
                     FFRSQEAGGKGPGDVWKLLIQDGTIMDPLLNLTQTISQHEHGARWKRNGVCVRAGR"
     gene            19653..19823
                     /locus_tag="VFR82_12490"
     CDS             19653..19823
                     /locus_tag="VFR82_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502461.1"
                     /translation="MWACQYVIRAVGQTEVDGFPDRAYFSREEAEAAALARAKWFIDE
                     AHLSKASVKTSG"
BASE COUNT         4454 a         5595 c         5906 g         4391 t
ORIGIN      
        1 tagccgaggc tctgaccaaa gtgacacacc tatgcggttc gatcacgcgt ggcttgcacg
       61 acctgcgatt aggggtcttc gtcatcctct ataggtgaca aggagggggg ctaactatcg
      121 agccaatgct ggcccctctc gacggtgttc gaagctaccg tcggtgcaag ccggatttcc
      181 cagcggtggc ccttctcgtc agttcgaaga atgaaatgaa tgacgcgatc gctttctcgc
      241 agtgtcggcg gtccgagagg catgaggcag cagccgaatg tggagggtta gtaggtgtag
      301 agccactagg atgttcctct cagcaaagtc aactcgtaag tttggcataa catcaggtcg
      361 gccatgcgtc ttcaaacgtt tgctgacatc tcgggcttag tctggcacaa tcatgttttg
      421 gcggttggga catggagaga tccgaaagac cgtgaattat ttgtggacaa agctcgctgg
      481 cagtgcaatg ggtgcagtag tgttttccgt cgctctgagc gcgtgccgac cagttccgat
      541 cgccacggtt caacctaaga gcgatcctgt tgtcgatatg tcagccaagg tgcaatctct
      601 ccagaaacaa attcaagagc gggataagcg tatcgaggag ttagaggccc aactggacgc
      661 actaaaactt attgaccagg accgcgagaa gcagagaaag ccgctccggg cgcccacgtc
      721 cgtggagccg attcagtaac gcgcgttcca cctgggcgac ggatgtgcaa ttcaacgctc
      781 ccattttcag cgagggagtt gtcgcattgc cagcagtctt gagcgcgtca cctcagactt
      841 acagttttcg gtcgtagctg aacattgaaa ggctcgccgt gccgtcctgg cctctcccca
      901 aacatgactc ctggtcgacg ccgtttgccg aatcgttgct tacgcatctc gatctttttt
      961 caggtgcaac gattctggac gtggcttcgg ggcatggcat tcctgccttc catcttgccg
     1021 agcaggtcgg gcgaactggg caggtagtgg gtgtcgatct gaatgcgcat caggtgagaa
     1081 gcgcgaggac gattcaaggg cagcagctcc catggttaca cttcgagtgt cttgatatgc
     1141 gcgcgttgcc atcgaacctg ccgagttttg atcggatcac cggtaatttg tcggtcatgt
     1201 tctttcggcc agatcggttt acgacgctac aaaatttgtc agcacacctc aagtcgggtg
     1261 gacaaatggt cttgacgttt ccgtcgctgg gcacattcga ttctctgtgg caaagggtgg
     1321 atcaagaaat ggcagcgcgc cgcttgagtg aggaacgacg acggcttgaa gcctatgtcg
     1381 gtgagcggcc atctgctgag caaggccgtg agtggctgca taagctcagg ttcgaacgca
     1441 tcatcgttag cgaatatccc ctagaagtcg cggttggcca cgggcgcgca tttttacatg
     1501 atccgctgtt acgtggcggg ttcctcgatg acgtgtatga gtgttttgac gacgccgtac
     1561 ttgccaatga aatcatgacg gcagtgtcgg aggacgtagc gagtttcgtg cctctgatag
     1621 cgctgcggtg cgtgctatct ggatggaagc cttcagggcg ggggtaggga attcagaaaa
     1681 agtagctgac ctctcgtgtt ttactttgtc ttcggttttg tgcctggcaa cctaccggtg
     1741 aacgtaccaa tcgccgctcc agcaacggca ataagaagaa tgatctctcg atatttattc
     1801 ccaaattctt gacctgcggc atgcccggct cgatagccct gatcaacatg tgtctctcgg
     1861 aacccggcag cagatcctag gacggctccg ccgataccta acatgccgaa atagagcaga
     1921 agggaccaga caaaacccat catgatgcgt ctcatagcgg cctcctcatg gtcttcgggt
     1981 aatttaatca ctgtagggaa gcaccgccta tgaaaatgtg aatatgagtt tgaggtgcgt
     2041 gttgctgagc aggcgtagac accggctgga aagcgacgcc gagtagggga ggcgcgacca
     2101 tcagcggctg ccgaaacgtg tgaaagtttg tctaaggtgg actgcctttt attaccctcg
     2161 cccgcaacat cctgtgatgc tcaattactt gggacgtgga aaggggaaag aggcctgtgc
     2221 gggccacgat ttcctgcccg tcgcggctat tcacgaactt cataaattcg tgtacctcgg
     2281 gatcccagtc tttgtcagga cgctgattga tgtacaagta gaacggtcgg ctaagcggat
     2341 actctccgga catgactgat gcagcagtcg gtgcaactgc tgtcctgccg cttttcccgt
     2401 tgactggcac cgctctgacc gccgatgttt caagccctat gtgactatag ccaatgccat
     2461 accggtcttt acctacagcc actatgacgg atgccaaacc gggttcggtt tttagggagc
     2521 gtttaaaatt gccattaagc agcaccgctt ccttgaagaa cgacctcgtg ccacttgatc
     2581 gatcgtgagg cccgtacagg tgaatggaag cgtcagccca agtgcctgcc aacccgactt
     2641 gaccccatgt tctcatgtcc gccatgccgc gcttatgagt cgacaaaaaa aattgccgtc
     2701 gatttgctct aacgtcagac tattaattgc attctcccga ttcacataga gggcgagcgc
     2761 cccgtaggcg atgacaaatc ctgtcggctc atgcccatgt ttcgaagtga atcgctcgaa
     2821 ttcttgctca gtaagtgcgc ggggagatgc caacagttcc acatcatatg acccgaagtg
     2881 gccagcagga tcaccatcac ctcggcgcat cccggatagc ccactgacga acgcatctaa
     2941 agggcttatc ttagatcccc gagggccttg caccgcaatc gtcgcctcgg gctgtcgcct
     3001 gcgaaattcc atggctagtt tcaacagcag cggtttcgtc gcgtccgacc cgacaatcgt
     3061 catgcgccca cgcacctgtg catgcgcgat gtaggcatcc atggagggat cgatcgagac
     3121 ccccgatcca gcgacgccct ccagttggcc gtaggaacga taagccaaca gagcaatcag
     3181 agctaccgtt ccagcgctaa aggcggatct taaccaatgt atgtttcctc tgtcctgcat
     3241 ctcaatttcg aagagggtcg atatagcagt ccagggacaa tcagaagtgt cctatgcttc
     3301 tcgtggtcgg tccttcttgt agggattctc cgagtatccc gagaagcgtc ccgtgactaa
     3361 aatgtaactc attatccgcc tccccaacta gaccgacgtc aggaggcaca taatgcgtaa
     3421 acgaatgagt cgccaagtgc aagaaggcgc tggtttggag tgcaattggt taaacgtgga
     3481 ggaacttgca gaggtggaaa tcacctccga ggattccgct catcctgtcg agtcggcctt
     3541 tgtcgctggc agcacttcag ggtggcgggc cgcggaaccc ggcgatcaga caatccggct
     3601 gctgttcaac cacccgcaac aactcaaacg catctggatc aatttcgtag agctggaaac
     3661 cgagcgtaca caagaatatg tcctgcgttg gtcatcggat aatgggcact ccttccgtga
     3721 aattgttcgg cagcagtgga atttcagtcc tccacacacg accaatgaaa tagaagacca
     3781 tcgtgtagaa ctgcctgcga cacaaatctt agagttgagc attaagccag acatcagcaa
     3841 tggagcagca gtggcttcgc tcgcgtgcat gcgactcgcc tagcgaacag cggttgtgct
     3901 tgcctctacc gtcacgatcc accgggacaa aagcttccga ctcgatacct aattgcgttc
     3961 tggttcatct aacaaatcag gccgataagg caacagcact ctaccagcaa gatcctctgc
     4021 accggttgaa gagtctggat atgtgtgcaa cttgtccatt tcatgagaac aagttgtctt
     4081 gaagttaggg cgagatggtc ataactgggc atatatatag cgagcgaaac gatgccggtg
     4141 cgccttttgc acataatcat cttacgtggc ttcatcccgt ctcctagatg gcgagaaacc
     4201 tttcacctgc aacaaaagga gggagtaaca atgcacgtcc accgcatcat cctcgggtca
     4261 gctctggtcc tacttgccgg ctgtcagagc atcggtgggg aaagcatgcc aaccttgact
     4321 cgcacgggcc aagtcaagga tgtcgttatc cgagatgtcg tcgaaccgac atccctgagc
     4381 gtaaaccccg gtgacgaggt ccgttggatc aacaagcgtc agggcgccgt tcgagtgatc
     4441 ttcttgaatc ccatgactga gaaccttaat tgtgtgcgta atttcggtgg accgcttgga
     4501 ttcggaacaa agcgcaacga gtactccgcc aaactcggct caaacgagac ggccagtgtg
     4561 tgtttccgcg attccggcga ggtgaagtat gtggtcaggg ccgaatcggc cagtgaccca
     4621 agcggagagc agaatatagc cggcacaatc tctattggtt ccgaagacca ggcaagccga
     4681 ccggtttcga aaccggtgga gaggaatgag ggcctggccc agtctgataa ggacgaggca
     4741 gcttcgagcc ggtagcgatt ccctagaggt gcggggttct tgtagggtgg gtttgatttt
     4801 cttggcgtcc atcgctgcga tggacgccaa ggatggattt accctcgtgt tctgccagtc
     4861 ccaccgaacc gacctaatct cccataacgc gggattcatt cgcatgcgag tgatcgcggc
     4921 tccggggtgt ttgttgcgag cacaagggac ggcatccggt gaattttcct tcgcccggct
     4981 cttgcacctt tgcgccaaat cgctacaatg cctccctcgg ttcatcatag cctcactcgg
     5041 gtcgtaaccc atcgtccggc aacgaggaca actcttggca caaaagaagt cggaacgggc
     5101 gggagcagcc cagcccttat ccgcctggaa gacggctttg cgagattggc aggcgcgcgc
     5161 ggtcgcagac gtgctgtcgc atccggctcc ggatttcttg gccatggcca cccccgcagc
     5221 gggtaagact cgctttgccc ttcgggtagc ccatgagtat gtgcgaacgc gcagggcgca
     5281 acgactcctg gtcgtgtgcc ccacgaatca cctgcgaagt cagtgggccc aagcggcggg
     5341 aaccgtcggt ttacatctgg atcccgctct aacgaacgag cagacttgtg aggcgcccga
     5401 ctatcatggc gcggtcgtca cgtatcagca agtggccctt gccccgcggg tcttccagcg
     5461 aggctgcgcc ggtagaaaca cccttgtggt attggacgaa ctgcatcatg cggcggacgg
     5521 ccggcattgg ggcagggcat tacgtcaggc gttcgatccg gcggtctttc gccttgtgtt
     5581 gtccggcaca ccatttcgct cagacaatca tcccatcccc tttgtccgat acgaacaggg
     5641 cgagagtcga gcggacttcg cgtacggcta tacagacgcg cttcgagacg gggtgtgccg
     5701 tccgattctc ttcccgagct atgagggcga gctgagctgg ctctcggagg ggcgcgaaca
     5761 ccgggcaacg tttgaagatg gcctcacctt cgagcgccag cgtgaacgcc tcaagacggc
     5821 gttgcttcaa gaaagttggt tgggtcctgt ggttgcagat gcgcaccagc agctgacgcg
     5881 actgcggaag caggagcagc cggatgcggc gggtctcatt gtcagcatgg accaagacca
     5941 tgcgcggtca gtcgccgcgc tcgtggagaa gtggaccgga acgaaagccg ccatcgccgt
     6001 ctcggatgat cctgcagcca gccggaccat caagacgttc gccgggcagc ggaagcagca
     6061 gtggctcgtg gcggtgaaca tggttagtga aggcgtcgac attcctcgtt tgcgggtcgg
     6121 cgtgtacggc acgaacgtgc tcacagaaat gtattttcgt caggtcgtcg ggcggttcgt
     6181 ccgcatgcag ccgcatgtgt cgacaccgca acgtgcgtgg ctctatttgc caaaggatcc
     6241 ggtgttggta cgatacgcca gacagatcaa agcggagcgc gatcatgtcc tcgaagacat
     6301 tttgcccgcg ggccagcggg atctcttcgg gcgcacgacg gtgtcgctca aagagtacac
     6361 cccgttgcat gccattccac gtgtcgattc cctcatcgga gaagacgagg cgcagcccga
     6421 cggcatggcc accgcgaccc ccgagccgcc gctgcatgac cgtaagcgag acctgcggga
     6481 ccttcatcgc gtgttggtgg ggagcttggc gcgcgcagcc ggcatcgatc accggcgctt
     6541 gaacgcggaa ctcatctccc gtaccggcag ccgcgtggac caagcgacct tggagcagct
     6601 cgaaaagcgt atccaattgt tgcagcggtg gcgcgaccgc gggtacgacg gaaaacgctg
     6661 acacccgcca tcagcacatt gttactcaac gctgctctga ctgaccgtct gcgtttcccg
     6721 ctacacgtcg tcaagggctc ttcctagtta cgacttcttg gtcaggcggt attcatagca
     6781 aacctcctca acggtgtccg aagggtgttg ttcaccggat gaccgggacg gcgagcaata
     6841 gattccggcg tgcttatcga gactagaacc ctttaggatt ctcgaacggg tatcgaacgc
     6901 gctagggaaa gtgatgttct gcgtggtggt ggcaagacag gtttcaacca ccggatcaat
     6961 acacctgcca ctggagatac gtccacgcat ggggcgaaag gcgagttcca tgaaggtatt
     7021 ggatgaaaca tgtggcggtt tccgagttcg acatggagca caagggtgaa cacggagcgc
     7081 atggaggaaa gacgcacttc aaaccgggtg aggtggagaa tctgccccag ccgccgacac
     7141 caagcggtaa ataatcgctt gtatcattcc tgttgtggtt catcggccgt atgactccaa
     7201 tgatggggag tgaagaacca atcgttgtct tccgcctggg accattcgtg gttctctctg
     7261 tttatctgac tcgaggaggc tgacatgtcg cagatgaccg cggttgaaat ccgttcacca
     7321 ggaacaccat tcgaagtggt catgcgagag attccagcac cagggccaaa ccgggtccgt
     7381 attagagtgc aagcctgtgg gatctgtcac agtgacatgt ttgtgaagga aggatattgg
     7441 ccggggctac aatatccacg cgtcgcgggt catgaggtcg ccggcgtggt cgacgaggtt
     7501 ggcgcgggcg tcaccgcgtg gaagaagggg caacgggtgg gtgtgggatg gcatggcggg
     7561 cattgtggtc aatgcgatcc ttgtcggcgt ggtgatttca tgggatgtcg gtacttccag
     7621 gtgaccgggt tccaggaaga cggcggatac gcgcagtaca tgatcgcaaa aagcgaagca
     7681 gtggcagcca ttccggatgc tctttcgccg gtcgaagcgg cccccatttt gtgcgcgggc
     7741 gttacgacgt ttaatagcct ccggcatagt gaggcggtcg cgggcgatct cgtggccgta
     7801 caaggcctgg gcgggctcgg ccatctgggc atccagtttg cgagcagaat gggatttcac
     7861 acggtggcaa tcggccgagg gaaagacaag gagcctctcg cactgaagct cggcgccgct
     7921 cgatatctcg acaccggttc catggatgtc gcgaaagaat tgacgagcct tggcggtgcg
     7981 tcggtcattc ttgcgacggc accggatagc aaggccatgt cggcgctcat tgacgggctg
     8041 ggggttggag gacaactcct ggttgtcgga gcatcggccg atcccatcac cgtgacgcct
     8101 atacaactca tcgggggccg ccggtccatt caaggatggc cctcagggac ggcccgggat
     8161 tctcaagaca cactgaactt ttgtgcgctc acgggaatac ggcctatggt cgagacactt
     8221 ccgcttgaac aagccgctgc cggctatgag cgcatgttga gcggcaaagc cagattccgc
     8281 gtggtgctta ctgttaccta actcatactc cactgtggag acgcctccgc ggcaggtgct
     8341 ttaggacctc cgaggtggca gtacctcgtt gatgtgacgc cattggttca tcaatgccaa
     8401 ctcggcctcg cccagggtgg tcgtgcgaaa ggtcagggtg tcgccgggtt ggagttgtgc
     8461 ggcctgcgga agatctgcag aaatgaccgt cgcgatcttg ggatacccgc cggtcgtggg
     8521 tcggtccgcc atcaacagaa tcggagatcc atccggtgga atttggagtg ctcccatcgc
     8581 cgttccatcc gagagccgag gaggcgtggg gtcctcgtga aggcgatgtc cttccaagcg
     8641 atagcccatg cgatttgact ggctggtgag tcgatacggt gtggccgtga gaaaggacag
     8701 cgcagcggca ttcacgtgtt gcggtccaga aatgacccgt aaggtcggtg aatcgacata
     8761 gagaggccga ttgccagggg cgagcattgc tccaatatgg ggccgctcac gctgatcaac
     8821 actcacccgc aacaggtcat cggccaaaag tgcacgtccg ctgaagccgc ccgtgcaact
     8881 tgaaatatgc gtggatcggc tgccccacag caacgcgaca tcgagtgcgc ccgccacggc
     8941 cacatatgat cgagcacccg ttcgtcttcg tccaaatgtc aggcggcttc ctgcctggat
     9001 ggctaccgtc gtccagatcg gcacgctgtg gccgtctata gccggcgaaa gatctgcgcc
     9061 agtgatcgcg aggaccgcgg cgtcctcaaa taacaactcc ggccccacga tggttatttc
     9121 gagcgcagca gccacatcgg gattgttgac cagacgattg gcgataacga gcccgagccg
     9181 gtccatcgct ccgctgactg aaacgccata gtgttggtaa ccatgacgcc cgagatcctg
     9241 gaccgtcgtg caccatccgg gcttgatcac tcggatcgag ctcgatttag cggtcgtcat
     9301 gactggcggc catcacttcg atcccattcc gttgaagttc ttcgcgaatg acgcgcgcca
     9361 gcacgagggc ctggggggta tcgctatgca gacacagact gtgaatcgac atcggaatga
     9421 gcctacggtc gaccgtcatg acagtttcat tgagtaaccg ccgaagctgc tctcgcacgc
     9481 catctgtatc ttcgatcagc gctccgctgt cgcttcgcga taccaatcga ccatcggctc
     9541 ggtatgcacg atctgcaaac ccttcctgga taatttccag cccggccgcc tttcctacac
     9601 gcgccaacat cgatccagcc agggcataca acaaaagcgc gccgtcaacg tcgcgcacgg
     9661 ccgagacaat ggcttctgcg atctcctcat ctcgggcggc catagtatag agggcgccgt
     9721 gaggcttcac atgccgcagc gtgaagcctt ccgtttttag gacacggcca cagaccatga
     9781 cctgctcact tacgagcgcg cggatttcgt tggccgtgac aggacgctca gtgcgtccga
     9841 aggtctcccg gtccggcaat cccggatgag cccccatggc cacaccgtgt tgctgcgcga
     9901 cagaagccgt cctgcgcata agatcccggc tgccggcatg cccgccgcag gcaatgctga
     9961 ccgacgtaat gagcggcatc aatgccgcgt cgagcgccat cccatcggcg gtgtcgcgct
    10021 cgcccatgtc gctgttcaaa tcaagtcggc gcagcattgg aaatccgggt aaactcgtcc
    10081 gcgtcaatcg gataaaactg cacgtgatcg cctggactga aaagaaatgg cgatgaggac
    10141 tgtggacgat agagactcac aggcgttcgc ccgatgattc gccacccgcc gggagtcgcg
    10201 gtcggataga tgcccgtttg cgattccgca atgccaacag aaccgggcgg gacaacggtg
    10261 cgaggcgtgg gcaggcgcgg catggcaata gatggcggga ccactcccat gtacggaaat
    10321 cccgggctga atcccagcat atacaccctg taatgggtag cgtgatgcaa ccggattgtc
    10381 tcggcaagtg attgtctggc gaaggcggcg acatcgtgaa gatcaggacc ccattcacct
    10441 ccatacaaca cagggatgcg gtgagcctga ccggaaagga ggtcctttcc atcggaagaa
    10501 agatcatgga gccgtgcaga gagagtggag atgggcagtc gaaacggatc gacatgaatg
    10561 gtgacagacg cgtacgccgg cacaatatca actacgcctt cccattgctg gattcgcacc
    10621 gcctgcgcaa atgcgagggc ctgctcatta agtcctggct cgatcgtgca gccaaattcc
    10681 acagtcacgg ccgcatctcc gaggggcagc accgcgaagc cattcctcgt catcggaaga
    10741 gctgatccat gagcaaccct aacctgttcg gacttgatga aggagtgggg ctggtctatt
    10801 cgaaagtgcc tgaccctgcg cacggaggtg attcgacacc agcccaatta caccttgagt
    10861 cgcttcttga ccgcttggcg atacgtccta taggctttcg atgttatttc aacttgctcg
    10921 tgcaatttat cccattctcg atcgtaggcg gtaggatcta ttggatctgg gcgaaagcga
    10981 tagatctctc cgtcatctga ccgaaacgtt gcaccgacag ttcgcaacaa ctgagacatt
    11041 tcccgagcaa gtgctgtggc tcgaacttga atcaccgggt gcagatactc gttctcaaga
    11101 cgctggaggg catctccaaa cttacacagg agttctcttt cttcaaaacg gaggcattca
    11161 gtgaagaagc tatagtccaa aatgttcttt acgcgtgatt catcgagtat gctatccaac
    11221 ctgtgaaaaa tggcaatgtc gcgctcacga tgcacgccaa tgagggaact gctcaatttc
    11281 cacatcgtct tgataagggg aacgattctg aaaggatcca ttgttgtctc tgacggtgtc
    11341 atgtttgggc tacatacgca gtgaagcgta atagtacatg aagtgaggca gcttggctaa
    11401 aggcaacctg taggaggaag tcgcgatgtc cacggtcagc gttcgttaca ttattgatga
    11461 cgtccctgct gcgatgcgtt tctatacgac gctccttggt ttcaccgtgg agcgggacgc
    11521 ctcccccgcc ttcgtatcgg tcgtacggga cggagtgcgc cttctgctga gcggggacgg
    11581 cagctcgggg aagcggccgt tgacagacgg ccgccagcag gtaccaggcg gctggaaccg
    11641 agtgcatttg gaggtgaccg atatcgctga ggaagttgcg cgtctgcgtg gggcgggggt
    11701 gtccttccgc atggatggga tcgtgaccgg accaggcggc gcacagatca tcctcgacga
    11761 cccatcgggc aacccagtgg agttgttcca gcctggacat tgagatcgcg tagcgcggcg
    11821 gtagcttctc gttcagcttc gcctgcgcat actccgtgtc tctggcacgg ggcgaagcga
    11881 acggccgcct ggccgctgcc ttctcgtgcg tttcctgcat ttgatgcatt gaagcccgcc
    11941 aatccttgct gttatctttc ttcatagacc gcgcgccgtg tgccttcagt ttggtctgaa
    12001 taggcctgag tgagcggcgc atccagcaga ttgcgaggtt cagagtggat agtcgaacgt
    12061 tgaaaatggc gatcgctgga ttggtagcca tcgtcggaat ctgcgagccg ttacaggctg
    12121 agccactctc agttggggca ccacccagcc taagaccagc gttcagcgag atcctgccga
    12181 tgttcgagcg agaatacggc gcggcagtca acattgtgta taccccatcg aaaacgctgc
    12241 ttcggcaaat cgaaaaaggc gcctccatcg atgtttttct atcggcgggg attgaagaag
    12301 ccgagtattt gcataagaag ggactcactc ttaatgggcg tcctcggatt tatgcgcaga
    12361 catctcttgt gcttgtcatg tcctcggatt ctcaagccac gcatatgact cttcgcgatg
    12421 cgctgaacag tcggagcacc cgcatcgcgc tcggtgatcc tcaaacctcc tcgttgggag
    12481 atatcacggc ccgggcgtta tcaaaaacct atccgaccta taaacgccga cccaacgttg
    12541 tctatgcccc gcacagcgag gacatcatgg atctgattcg caaggggaag tctgatgtcg
    12601 gtctggtcta tcgtgtcgat tcgatcaaca gcggctatgt ccgcatcagc gacgaagacc
    12661 ctttcggcgt gttggtgcca gttcagttcg ggcaagcggt cgcctcaacc tgccgacctg
    12721 ctttgcgctc ggctgcggac cagttttctt cttttttgat gacgcctcgc atacagaaac
    12781 tgttggtgaa atacggattt gactcggttc cgttaccggt gagtcacatc cccacggaga
    12841 gaaaagagag ggcatcatga ctaggaaagc tgtgacttgg attaccgtga tgacaacgat
    12901 ggcggcgctg gctgcgccga gcatcttcgc catcgagaaa gtggcagggc cggtcggtgt
    12961 gcctcccgaa atcgtggcta actatttgcg cgccgtcatc atggcccacc gccattttta
    13021 caccattcac attgccaatc gacttcgcca agagcgcatt gttgatgtct cggaaaactg
    13081 gcggacgacg cattcgctgc ctttacccgt acagctcgtg cgagagacaa gcgagattgc
    13141 cgaactaacc ggcccgaatg tccgctaccg cttgatcagt caatggccga tcaataaggc
    13201 caatgcggca agcaccgaat tcgagcggcg gggtctgaag gaagtgcagg cgcgtcccaa
    13261 tcgaccgtat accagctcaa gtactgtgcc gaccaacgcc gaacagctct ttgaagcggt
    13321 ctatgccgac gttgctgtca cgtcggcgtg cgtcgaatgt cacaacacgc atccgagcag
    13381 tccgaagaag gattttaagg tcggtgacgt catgggagga cttgtcatca gcttcccgtt
    13441 gggcaaacag tgagcagccg gtccgccact cgccggaaca tggtttctgg tatgtgtgca
    13501 attcctttct gatgctgatc aatattcgcc gttctcgaag atgccgacca ccgaacaaac
    13561 ggagacatgc tcttaccgac tgattgcatg ggccccgttc ggaatcaccc tgggactcat
    13621 tgtttcgatg atgcttgaga gagaggcgtg gttgtatgca gcggctgtcc ttggtgtact
    13681 cgcttcgata gcagtaggtc ggacgtcggg aaaagtggaa gaggctccag tgcaagcgcg
    13741 tctgtggaaa tttgccaaaa gctggctgct ggtcgtcgcc ttggccgccc tcagtgttct
    13801 tcacgggaaa tggcagccaa tggtcttctt cgcctttgtc ggcgtcatgt catcagcctt
    13861 gttctggtgg ggctgcaggt cgagcaaata ggcctggccc gcgcgacgcg ccggaaacgg
    13921 atctgatacc ttttcgtctt gcgcctcatc atggcttcaa gaatcttgcg atctcgtccg
    13981 tcatttcttg ctgatgcccg acccacaagt gaccgcctgt ctcgtacccg acaaagcgtg
    14041 ctcccggaat atgctcggcg gtatagcgcg caccatcata ggttccaaat acatcgtcag
    14101 cacaactgat ggtcaaggtt ggaacgtgaa ttttctcaag ctcatagcgc tggagtgaag
    14161 gtatgacggc cgcttcgttg agcaatccaa gtcggcgttg tgcaatcggt aatatgtgtt
    14221 ccagcgcccg ctgtacgcgc gcttgctctt ccggggccgc atccctgacc aggtttggcg
    14281 gcgtcgccag gatgtaacgg atcgcggcgc cgggggcgaa ccggctcaca gcccaaaaca
    14341 cgacgtccca cttcaacgca gtatcgaata agaactgggt tccaggcggt gtgtgcaggg
    14401 ctggtgcgtt gcctggtcgg gggacaaaag tcgctggaac cacgagtact aatgcggtca
    14461 aacgagcaga atgccgcaga gcaaactgca tcgaggatgg ggcgccggcc gagccaccga
    14521 ctatcgcgac cttctcgatt tcgagcgcat ccaataggca cgcatgcgca tcggcctgtg
    14581 ctgccggtga agcatctggc gggagcggtg tgcgcaggta cccgaagcgt gacatcgcga
    14641 tgacgcgaaa tcccagcttg gcgagcggcg caccgaagtc caatccttgg tcgaaccccc
    14701 caccggcgcc atgcacggcc aacacaggtg gaccatctcc tgcaaaggcg tattcaatcg
    14761 gaccgcagga ggtatgggca atgctgctgc cagtcgcgac gcggtcgcgt gcctcttgaa
    14821 tagctgtgtg gtagaagtac gttgccgttc ccagtgcggc aatgaccgcg cctaaagcaa
    14881 gcagtaaagc cgtgcgcaca ggtcggctcg tcatgtccac tcgccccgct cagagatcgc
    14941 tgacccattg ggcacgagca caatcttacc tgtcacgcca cccttctcga ggaactcgtg
    15001 cgcctgtctt gcctgaacaa gcggaaatcg ctttgcgatg agtggcttaa tcttcgcttg
    15061 gtgaaggagc tcaaacaaag cgatcagatt ctctcgaaac agcacaggcc gcagccgttt
    15121 gagccactgt acgctgtagg ggaccacgcg tttccggcca ggaagaatcc agccagcggc
    15181 aatgtacagg ccgaagatag cgatcgcacg aaagcgatgg cgacctcgtg aacggcctga
    15241 agtcattcgc cctccatata gcgatgaggt aatgccatag gccacgactg tcccgccgga
    15301 acgtagggcc ttgcgggaac gccagatgtg cgtgccaccg atgccgtcga agacgacgtc
    15361 cacaccctcg ctcgtgaacc ggtgaatttc tgccacgaag tcctgatgtt ggtaatctat
    15421 tggtataccg cctagggtgg aaacggtcga tgccccttgc gaagagcagg ttccgtacat
    15481 ctgcagccgc gcgagccgac caagctgcaa gagtgccgaa ccgactccgc ctgtcgcgcc
    15541 gtgaatcaac acgcgctggc ctggtctgac ctttgcggag cgatgcagca tctggtatgc
    15601 agtcacataa ttcagcacga ggctgacggc ctcggcagcg tctaattcag gtggcacagg
    15661 aaccagttcg cgttgcggca agcagacgaa ctcggcgtat gcgccgttga tcggcagcgc
    15721 ggccacgatc tggcctgttt ccaacataga gatgccgtcg ccaatccgat ccacgacgcc
    15781 taccaaatcc catcctggcg tgaaaggcag ggtcggcgtc tcggggtgaa tgccctcccg
    15841 catcatgacg tcgggcaagg acacacctgc cgccagcact ctcacccgca cttcaccttc
    15901 ctttggctca gggcgatctt cttcgagcac ctgaagggca tcaggcccgc cgtagtgagt
    15961 cacgatgatt cgggtgtgtc ggtgttggtc tttcatagga ggtctattcg atcaggtgca
    16021 tcagtgcggt gagttccaca gggagaaagt gtaatacgct gtggagagca ctttcggatt
    16081 cagttctacc aaaggggtga aaacgactcc cttgttcatc gctgctttca gcctttttcg
    16141 atctttgtgc atgggaaggg gacctggccg gcccgccgcg cgccggtaaa tggaaatgcg
    16201 ttgcttggtt attgagcggt tacatgttga agggcggtca tcggacctga cctccgcagc
    16261 gtcgcgagtt gcgatctgtc cttgacgagg aacgcaccag caaaccctaa ggcattcacc
    16321 gaaatgcctt cgaagcattc ctgcgaccgt ggcgctacca acatccaccg gcgagtcatg
    16381 agcaggttgt aaggagcggt taggtcatga ctgcctgagc tcgcgtcgac ggacaggccg
    16441 gctgcgtgaa gcaatttgcg gtaacaggca tgcaaggaag cgccgctgtc ctttccggga
    16501 tttgtccagt caggatccat tggcgcaaag gcatggcgga aaggcaagcg tggaacggta
    16561 ccgatcatgc ggtccgtctg agcaaagcgt aagagtggct ccatgggcag cggtgcaatg
    16621 ggggcgtcga gcggcaccaa ttgtagatgc ttgtgcaact tgctcgctcc ggccacctgc
    16681 cccgcgttgt agaaggcgag ggcatccatc tcagtgagac cgatgaggag ggcttcgcag
    16741 tctttctgcg tgagctgaca tcgctgatct tcgaacgcgt tggtggcgat caacagatgg
    16801 tgttcgaaca cgctgaactt gttcaggagg gcgatgtggg tcgccgaaat ggatgcgaca
    16861 aagaggtcct gatcgtatgg aaacggattg accggtgatc gagcggcttc ggtgatcgcg
    16921 ttcatggccg gggattccag cacacgcact tggaatggga tgctgtcctg ctcaatcact
    16981 tctagagtcg tcggcaggaa ttgtatggcg ccgcgccggc gggctgttgc cgtacgttcc
    17041 aacagggagg cgcgcaacgt gcctggcgtc agctgtgaat cctgatcagc cataagagcg
    17101 atggtacgcg aagcgaaggg ggcgggaaaa gcctgatgtc ggaagacgaa gtcgcgggaa
    17161 tcggtgaggt agggcttagg gtcgaaccat gacagatctc gctcatgccg gcggagcctg
    17221 tcggacggaa gcgcaagcct gagtcaggtc agctattgat cgcgaccact attgccccca
    17281 caagggccat gagggccaaa agcacctctc tgtcgccgca acggttgaag agggtacgca
    17341 cgtcggatct ccttgagaaa cttcccttaa aaataggagc aagtcagatg ccggcgggag
    17401 agagttaacg atttccgctt tttagggcac tgaggggatg caaaatcgag cgattctgca
    17461 caggtcgcgc tgtgcgaaag agcgagtaat gtgaagttgg gggagcatca gcacgacgcc
    17521 atggtgcgct ctcttggagg gatgagggag ggacaagttc agggaagtaa attgctgata
    17581 agaggatcaa tttctccatg acaccttcga atctgcacac tcgaatccga tgtctactcc
    17641 ctcaagtctc gggtgaaccg catcgaatcc accgaagcga actattccta ggtctgtgct
    17701 ccggcactat ctgcctgctc ttacacagac accattgcgc ttccacctcg ccccatgttc
    17761 gtgttgtgaa atggtttgag taaggttcag gagtggatcc ataatggttc catcctgaat
    17821 gaggagtttc catacatccc cgggtccttt cccgccagct tcctgacttc gaaagaagag
    17881 gatctctttt ccactgtggg aatactccgg tttttggtca tattcattgg cgtttggagt
    17941 cgtgactggc tgtatatggt ctccggctcc gtcgccatcg gagtcgaccg cgtccatttc
    18001 aaacaaggcc gcacgactcc catcctcgga ctgcctcgca aaaataatcc ggccggaggg
    18061 cgaccaagac ggatcagtat cgttcaccgt gttattcgtc agtcgaacat ccagtccgga
    18121 gtttagattg tgaacaaaaa tctcccgtga ggcattcacc acgttacgtg taaaggcgat
    18181 tttggtcccg tcaggagacc atgaagggga gagatcttcg aaattgccgc tcgtaatgga
    18241 cgtcaccgtg ctggtgctca cattaagttg gtaaatgttg taatagcttg ggttgattct
    18301 gttagatgaa aaggccagca ctgcgcaatc ctgcgaccat gccggaaatg aatcgtcccc
    18361 cgcgctggtc gtcctccgtt ggatgtcctg agcagagtag gtgtggtcgc cgccggcgat
    18421 catccgcgcg gtgtagatct caaggttgcc atcccgcatg gattcaaacg caatgtactg
    18481 cccgtcaggc gaccacgtca gggaactatt tccctcagca tctagattca ggagcgattg
    18541 gcctgatcca tcgtggtgca tgatccacag agcatctgtt ccaccaagct gcggcgcccg
    18601 agtaaaccca atgtcctcgg cgggcattct tggaggagag gacacacatc ccgaaaggaa
    18661 catcatcgca aagagagtac ttccatgagc cagccaacgc gtcatcgagc tcccctttac
    18721 tgaatggccc gcagcacatt ctacttttaa gtgcccgtgg ggtctatggt gattcgtgaa
    18781 gtgacagcag aagggagatt cctcaaaccg aagtgggtat ccgtgcgaag cttgaccggc
    18841 ggacgaccga cggttcattg aaggccacca gttcccgcca tgcgggcttt tcctgttcct
    18901 ttgcccaccc tcgggcattc gctagtccct tccccccttt cgttgtttca tactgggagt
    18961 aacatgcagg ccttttgtcc aacgctcagc aggagtgctc gattgatgcc tatggcgcct
    19021 atgccaattg gaagaccggg gcgctgttga aacacacgaa ggactcccat ggcgaggttg
    19081 atgacgcggc agcgtaacga tgcctacagg attcacgtcg cgtgagagag gacagatagg
    19141 attacgccgc cactctcatc gactgaccta atgcccgcgc gaagccccta ggagatttgt
    19201 atgtgaggac agaagtggag gcatgagcca tgaatgccac aaccgccttc ctatgtatca
    19261 cgacattgag cctcgtcagc ctcaccatcc ccgcacactc ccagaccaat gctgcctcgg
    19321 tgtcagaagg agggtcggct tctcatggaa gtcgggaaac agaaagacct gcgatgccac
    19381 ggaaggcgga ctccgacgtg acgcggggag ggatgtcatc agccggtaag ccgaaacaga
    19441 taccagcgcc gctcgatcgg agtcaggtag ttgaagagcg cttgcgatcc ggacagatgg
    19501 aaaaaccgat cgcgcaaggt gaagtgagcg accgactgaa ccaattgtac agtggatcga
    19561 acagccagat cagtagagag tcgaggtgaa ctatgagcct acatgactat aaaggacatc
    19621 agattacatt gataccaagt ccggaaggtt cgatgtgggc gtgtcagtat gtgatcagag
    19681 ccgtgggtca gacagaagtc gatggctttc cagaccgtgc ctatttctca cgtgaagagg
    19741 ccgaggccgc tgcgctggct agagcgaagt ggttcatcga cgaagcccat ctcagtaaag
    19801 ccagcgtcaa gacttcagga taagcgttat aaacgactac ctgacccggt tcgcgccgcc
    19861 ccggcgtcaa cgccacgagc gcatccttcc ggacgcttgc gataacgctg ccgcggtcat
    19921 gttggacaat atgctctggc acgcggtttt tttaccatga cactctgcaa tatctcggcg
    19981 acccgacttt ctaatagagg tccttaggca gccctttgag cgtcctggtg tgatgatagg
    20041 tgggggagta tgaaatcaga aggagaccgc tccacacttt cggtggataa gtctgtggac
    20101 aaggcttttt taggaggcta ttagtccaat tcccaaagcc gaatgaccaa attgcctatt
    20161 ttttaagcat agccagcgtg gggatagata ccccaatatc ttgcgattga ctttttaata
    20221 cgtctcaaaa cctaccatta gttaactcga gacacactgt ccccgttcca cttgggcaaa
    20281 gatacgtaat ccggttggaa ttgccttgca ctgagccact tatccacatc cttctagggt
    20341 tcaacc
//
LOCUS       DASTQY010000384         3597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_886651, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000384 DASTQY010000000
VERSION     DASTQY010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886651"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VFR82_12495"
     CDS             <1..233
                     /locus_tag="VFR82_12495"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEU4502462.1"
                     /translation="HYEYLVRATDPDGDPLTYALEVSPPGMTIEAETGQIRWMISQET
                     TGSQRVRVVAKDPQGGFATQDFDLSLNKPTKS"
     gene            complement(338..3202)
                     /locus_tag="VFR82_12500"
     CDS             complement(338..3202)
                     /locus_tag="VFR82_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HEU4502463.1"
                     /translation="MLTESKLTDGKLRLFDANRLEHEGIGINDVQVLFQSHPTRTQAD
                     LMLTGTTEDGGDLHIAGTLALQQHGRSNASIPRQFDGILRFHQWDIAYWLNRTGQSAA
                     TSTQTTTRRGNISAGLHLDFPPDAKGFNVIVSDLNTDLGWLLIRGHIMVEGGGTDHPA
                     YAVKLSTSPVSSKTFFDHIPPSWIPKTIHAAVDEHELTGTVELESVTLRGRIDVLRVP
                     DEWQVAAKVLHASGGWEKTRTLIRNVSVTVWLDSTRANITDLSGDMNGVHVTSSKLSV
                     SDLNLRPIVDARFLAEGPLDNVMAVVEHFSEGTGAHDALRTVRDATGTLQVAVHVNGP
                     IIPRPNLRMISAELYLQDMGAHIAKAMTIGQLNGSFAADSRLVGIKHVRGVVQGIHFE
                     AEGNIDVAAPNRVNTLKVNMSSDGTAIQELLATYLPALSHMHIDGPAHSTMFLSGIAA
                     LVHCRGTLDVTDAEIIVPSLLDKKPGVPGLIEWEGKLFDGKRVVVDHLRLVVPHGEIR
                     AAGQLELGHTPKFHVKLNAGPLSLRALQAIGVGTPISEGMLDASAFVSGEGTNWKLWV
                     PSGWVSIHRAVVTLPGLDEQLRELSGRAQLTQGGLLVNELLFTMGESDFKLTGMVEHW
                     RTQPRATFMVESSQLNVSDFVSKKTVDPDKRGSHVQEWIGSKKAAITFLVKQLRYDRL
                     VLKTVSGEITVDQHKAKLNGLRGETKEGVLSGQVEGRFGAHNQMEIAAEMSVDGIPAQ
                     QVFSATDDNKERLQGNVSLKGVLQARIDATSPLQHTLSTGRDGIVVKVTKGRLQQDPV
                     LTKALKILNLPSVLFGQVDFDVRGIPFDSLSARVVGSNGVFSSEDIVLNSPVIKVTGA
                     GSADVKDNGLDLAVAVSPVAAYSDLAGKIPLFGQLFGEDHQGLTTAVFEAKGSLQNPD
                     VAYLPLESLARGLTGYPRLAIDVLVNTIRLPPTALAYATE"
BASE COUNT          845 a          981 c          922 g          849 t
ORIGIN      
        1 atcattacga atatctggtt cgtgccactg atcctgatgg agaccctctc acctatgcgc
       61 tggaagtgtc gcctcctgga atgaccatag aagccgaaac cggccagatt cgttggatga
      121 tcagtcagga aacgacaggg agtcagcgtg tcagagtcgt tgccaaagat ccgcagggtg
      181 gtttcgccac gcaagatttt gatctatcgc tgaacaaacc gaccaaatcc tagttgatgt
      241 ggactaggat tctgcagacg agaacctcac ccgccggaag atctcttcac acgaatcctc
      301 gtgcctcgtg catcactacc agcgcgtaag caatcgctca ctctgtcgcg taggcaagcg
      361 cagtaggagg cagtctgatg gtgttgacca acacatcaat agccagacgg ggatagccag
      421 tcagtcccct tgcgagcgat tccagaggta gatatgcgac gtccggattc tggagcgatc
      481 ctttcgcttc gaacaccgcg gtcgtcaatc cctgatgatc ttcgccgaat agttggccga
      541 agagtggaat cttccctgct aagtcggaat aggccgccac tgggctcacc gctacagcga
      601 gatccaaccc attgtccttc acgtccgcgg atcctgcacc agtcacctta atgacggggc
      661 tgttcaacac gatatcttcg gaagaaaaca ctccattgct gcctacgact cgagcagaca
      721 gtgaatcaaa gggaattccc cgaacgtcga aatccacttg cccgaaaagg acagatggga
      781 gattgaggat ctttagggcc ttggtcagca ctgggtcctg ttgcagcctc cccttcgtta
      841 ccttcacaac gatgccgtca cggccggtac tcaatgtatg ttgaagaggc gaggtcgcat
      901 cgattcgggc ctgaaggaca cctttcagag agacattccc ttgcaaacgc tctttgttgt
      961 cgtccgtggc cgagaacact tgctgggctg gtattccatc cacagacatt tcagcagcaa
     1021 tttccatctg gttatgcgca ccgaatcggc cctctacctg tccggacaac actccttctt
     1081 tcgtttcccc tcgtaacccg ttgagcttgg ctttgtgctg atcaaccgtg atctcacctg
     1141 aaactgtttt gagaaccaac cggtcataac gtagctgttt gacaaggaat gtgattgccg
     1201 ccttctttga tccaatccat tcctgcacat gactccccct cttgtccgga tcgaccgtct
     1261 tcttggacac aaagtccgag acattgagtt gggatgattc caccataaaa gtcgctcgcg
     1321 gctgggttcg ccaatgctca accatgccag tcaacttgaa atcactttct cccattgtga
     1381 acagcaattc attcacaagg aggcctcctt gcgtaagctg tgctcgtcca gaaagttcgc
     1441 gcagttgttc gtcgaggcca gggagagtca caacagcgcg atggatgctg acccatcctg
     1501 aaggtaccca taacttccag ttcgtgcctt caccgctgac gaacgcagac gcgtcgagca
     1561 tgccctcgct gataggggtt cccacaccga tcgcctgaag tgcacgcaat gacagcggac
     1621 cagcgttcaa tttaacgtga aatttgggag tatgacccag ttccagttgt ccagcagctc
     1681 gtatttctcc atgcggtaca accaatctca agtgatcaac cacgacccgc ttcccgtcga
     1741 aaagctttcc ttcccattca atgagccctg gaacacccgg cttcttgtca agaagagatg
     1801 gcacgattat ttctgcatca gtgacatcca gcgtgccgcg acaatggacg agcgcggcaa
     1861 tacctgacag aaacatggtc gaatgggctg gtccgtcaat gtgcatatga gataaggccg
     1921 gcaagtaggt cgccaagagt tcttgaatgg ccgtgccatc tgacgacata ttcaccttga
     1981 gggtattgac ccggtttggt gcggctacgt cgatgtttcc ttcggcctca aaatggatac
     2041 cttggacgac accacggacg tgtttgatcc ccaccaaacg agagtcagct gcaaaacttc
     2101 cgttcagttg gccgatcgtc attgctttgg cgatatgagc acccatgtcc tgaagataaa
     2161 gttctgcgct gatcatccgc agattgggtc ttggaatgat gggtccgttc acatgaaccg
     2221 ccacctgaag ggttcctgtg gcgtccctga cagttcgcaa cgcatcgtgg gcccctgtgc
     2281 cttcgctgaa atgttcgacc acagccatga cgttgtcgag cggcccctca gcaagaaagc
     2341 gggcatctac aatcggtctc aaattcaggt cagagacgga gagcttgcta ctcgtgacat
     2401 gcacgccatt catatcccca gaaaggtccg ttatgtttgc acgcgttgag tccaaccaca
     2461 ctgtcacaga cacgttgcga atcaatgtgc gtgttttctc ccaccctcca ctggcatgta
     2521 gtaccttggc cgccacctgc cactcgtctg gtacgcgaag gacgtcgatc cttccccgta
     2581 aggtaacgga ttccaactcg acagtacctg tcagttcatg ctcgtcaaca gcggcgtgta
     2641 tggttttagg gatccaagag ggaggaatgt ggtcaaagaa tgtcttagaa ctcacaggag
     2701 atgtcgaaag cttgactgcg tatgccggat gatcggtgcc tccgccctcg accatgatgt
     2761 gcccacgaat cagtaaccat cccagatcag tgttcaggtc agagacgatg acgttaaatc
     2821 ccttggcgtc aggcggaaag tccaggtgca gaccggcgga gatgttgcct ctcctagtcg
     2881 ttgtttgagt tgaagtcgca gcagactgac ccgtgcggtt caaccagtat gcaatatccc
     2941 attggtgaaa ccgcaagatc ccgtcgaact gtcttgggat agaagcattc gagcgaccgt
     3001 gttgctgaag ggccagcgtg ccggcaatgt gaagatcacc gccatcttct gttgtccctg
     3061 ttagcatcag gtcggcttga gtccgagtcg gatggctttg gaacaacact tgaacatcgt
     3121 tgattccaat gccttcatgc tcgagacggt tcgcgtcaaa aaggcgtaac ttcccgtctg
     3181 ttagcttcga ttctgtcaac atccatcgct gaggggaggt actatctcgt ctctctgttc
     3241 tatgtgcctc gagtaacggt aggtgccagt gtccattggc atccctgcgt atcacgacct
     3301 caggttcgct ggcatggagt gtcagcatcg gtactcgatt catgattgac gaccaaagac
     3361 gtaaggagag cgaagcgcga gctgctcgca agttaggctc gaaggtgagg gcatctctca
     3421 ccaataaatc agatacctcc atgcgcactg taggaaatag actcacatgg agcgtgctta
     3481 tctcaatccc cagttggttc ttgagagtct gcacagtgag gtcgtgaagg cgtggatggt
     3541 taagaacgag gctcgctgtt gagagagata aaatgaaaaa agcggtgaaa acgagcg
//
LOCUS       DASTQY010000385         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1512294, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000385 DASTQY010000000
VERSION     DASTQY010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512294"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFR82_12505"
     CDS             <1..367
                     /locus_tag="VFR82_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502464.1"
                     /translation="DGAQAPASSRVHPLSSLPFSFKTVLASVDLVMTKPGYGTIVEAV
                     ALDLPVLYVRRYNFADEGPLVDFLLRHGCGHELSREDFFSGNWRPALDALTERKAEGV
                     RPSVTGATDAARFLSQYFQ"
     gene            510..1109
                     /locus_tag="VFR82_12510"
     CDS             510..1109
                     /locus_tag="VFR82_12510"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4502465.1"
                     /translation="MDTPTRRATSAIDPALLASVAKGDQQAFSQLYDHSSTLLYTLAF
                     RILGNHEEADDLLQDVYLEVWRKVVRYDVGRGTPIAWLITLTRSRAIDRLRSRNTRGH
                     NATVGLDTTASRVADLGPSPFETQADQELRMAVGNAMTILPPAQQQALELAYYEGLTH
                     SEIAARLNQPLGTVKTRIKLGMSKLRDSLRHFWDQGDIG"
     gene            1106..2113
                     /locus_tag="VFR82_12515"
     CDS             1106..2113
                     /locus_tag="VFR82_12515"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEU4502466.1"
                     /translation="MTHEELEEAVPLYATGALDRAERQALEAHLLSGCPSCHSALKDY
                     QSAASLLPFGLSPMQAPRELKSKIMGARNPDPIADDRVFKDSARPSLEPGEWMNHLFP
                     PIAPARSHSLPWAIGLGTLLVVGLGSYFGWTYATRISDDLAKIRQLETSLQQQSDRMA
                     TLQKDLRVRDRSLAELQVDAQRKANDAIELKSQLIEREVELEDVREQLTAAGRGKGQD
                     ELATLLRRPDATVVTLAGSDVAKRASAVLLYNPATEKIWLYSANLPECPKGTAYQLWA
                     IDKKTVSLGTFHMDAGQTAHLLVRRMPEFEKAKRFAVTLEPRIGSPHPNGPIYLVGEN
                     S"
BASE COUNT          490 a          690 c          603 g          444 t
ORIGIN      
        1 tgatggtgcc caagcgcctg catcctcacg tgtgcatcct ttgtcgtctc tccccttctc
       61 cttcaagacg gtgcttgcct cggtggacct cgtcatgacc aaaccgggtt atggaacgat
      121 cgtcgaagcg gtcgcccttg acttacctgt cctctacgtc cgccgctaca actttgccga
      181 cgaaggcccg ctggtcgact ttttgcttcg gcacggctgt gggcacgagc tgagccgtga
      241 ggacttcttc tccggcaact ggcggccggc ccttgacgcc ctgaccgaac gcaaggccga
      301 aggcgtcagg ccgtcggtca caggggcgac ggatgcggct cggttcctca gtcagtactt
      361 ccagtgacgc ttccagcctc acaaagcaac tcattgatat cacgctatga tgtcgcctat
      421 taaaatacac atccttttgg cgagactgcg cgtagatgct atagttgtgg cggacgtgaa
      481 gtgatcctac ctggcatcag cgcttcttta tggacacacc gacacgacgc gccacctccg
      541 caatcgatcc cgcgctgctc gcgagcgtcg cgaaaggaga tcagcaggca ttcagtcagc
      601 tgtacgacca ttcgagcacg ctgctgtata cgctggcgtt tcgcatattg ggcaatcacg
      661 aagaagccga tgacctgtta caggacgtgt atctggaagt ttggcgaaag gttgtacggt
      721 atgacgtcgg acgcggaaca ccgatcgcct ggctgatcac tctgacccgc agccgtgcca
      781 tcgatcgttt acgttcaagg aatacaagag gccataatgc gaccgtagga ctggacacta
      841 cggcctcacg ggtggcggat ttgggtccaa gcccttttga aactcaggcc gatcaggaac
      901 ttcggatggc agtcggtaac gcgatgacga tccttccgcc cgcccaacag caggccctcg
      961 agctggcgta ttatgaaggg ctgacacaca gtgaaattgc cgcacggctg aatcaaccac
     1021 tcggcaccgt caaaaccaga atcaagctcg gtatgtcgaa gctccgggat agccttcgcc
     1081 acttctggga ccagggtgac atcggatgac gcacgaagaa ctggaagaag ccgtaccgct
     1141 atacgcaacc ggtgctcttg accgtgcaga acgccaggcg ctcgaggccc atctcttgtc
     1201 cggttgtccg tcctgtcata gcgccttgaa agattatcag tcggctgcct ccctccttcc
     1261 cttcggtctc tcccccatgc aagccccgcg cgaacttaaa tcaaagatca tgggcgctcg
     1321 gaaccctgat ccaattgcgg atgaccgcgt cttcaaagat tccgcccggc caagcctcga
     1381 acctggagaa tggatgaacc acctgtttcc gccaatcgcg cctgcccggt cgcattcatt
     1441 gccatgggca atcggactag gaactctgct tgtcgttggg ttggggagct acttcggctg
     1501 gacctacgcg acacggatct cggatgatct cgcgaagatt cgacaactgg aaacctccct
     1561 gcaacagcag tcggacagga tggccactct gcaaaaggat ctcagagtga gggaccgttc
     1621 cttggccgag ctgcaagtgg atgcccagcg aaaagcgaac gatgcgatcg agctcaaaag
     1681 ccagctgata gaacgcgagg tcgaactcga agacgttcgc gaacagctca cggccgctgg
     1741 gcgcggcaag ggtcaagacg aactcgccac gttactccgt cgaccggacg ccacggtcgt
     1801 gactctggcc ggttcagacg tcgcgaaaag agccagcgca gtgctgctgt acaatccagc
     1861 gacagagaaa atctggctct attcggcgaa tctccctgaa tgccccaagg gcacggccta
     1921 tcaactctgg gcaatcgaca agaagacagt cagcctggga acctttcata tggacgcagg
     1981 ccagaccgca catctcctgg tcaggcgaat gccggaattc gagaaagcca aaagattcgc
     2041 cgtcacgctt gaaccccgca ttggaagtcc ccatccgaac ggccccatct atcttgtggg
     2101 tgagaactcc tgagttcttt tctcacccgc ccaccctcgc gcgccaagac gcgctcctcc
     2161 catccagctt gaccccgccg ggaaagctcg ctacaaccgg cgcgatgatc gatcctgcga
     2221 ccacctc
//
LOCUS       DASTQY010000386         6086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1306960, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000386 DASTQY010000000
VERSION     DASTQY010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306960"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="VFR82_12520"
     CDS             complement(<1..1206)
                     /locus_tag="VFR82_12520"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU4502467.1"
                     /translation="MKLESEDGNASAENSKLFRHGFRAKPIILQLQIYGLCGITFLSF
                     FPSLFHFQEYAFFLLFALALALARVEKFNPYVRTRLDLPLFAFISWVLFTVPFSIDPA
                     YSFSEWRKFVAHMLVFYWAMFVLRLHGDVEPGRKVLLAIVLGSFVLSLFALEDFLSRG
                     GTWRDRLVRAAAPYSDYNWLTTYLVLVIPVLIGGLLIESSLAPRALGLCSLATSLLAQ
                     IAAYTRAGWVAHFVQALGFVLIIHRQQWLVWFLVGAILAGSAALFTLASRGYQQETLD
                     PWTLSARVKTWHLGFEQVVEHPLVGIGFGNDTFSKVYTAEIEADKSKGPVAKVLPALH
                     NTFAMVLLGSGVPALLLFVWTIVRAIRKLISGIRELSFQHAGMLLLPAAIALSVLGFV
                     VRNTFDYMFA"
     gene            complement(1251..1973)
                     /locus_tag="VFR82_12525"
     CDS             complement(1251..1973)
                     /locus_tag="VFR82_12525"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4502468.1"
                     /translation="MSEEILRRHQAVWKQKPILRRLYASWYRQITGWLQPGVTLEVGG
                     GTGNLKEFSPHVLCTDVVRMPWLDALADAQHLPFRSRSLSNIVLFDVLHHLENVRYFF
                     DEALRVLIPSGRIVIMDPYVSVLSWPVYRFFHPEPMDFSHDPLTIIPTVQRQPFDANQ
                     AIARIIFERSYDRFHQLYPHFVKLAHERLAYFAYPLSGGFDRRSFLPLWSIDMVLRLE
                     QWCRYFGRLFAFRLLVVLEKSV"
     gene            complement(1974..3395)
                     /locus_tag="VFR82_12530"
     CDS             complement(1974..3395)
                     /locus_tag="VFR82_12530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4502469.1"
                     /translation="MERDQRRKHEIAERFDRLSSQRTRWQEKARYYYEDQLRYLRFLV
                     PEGLSILEVGCGLGDQLAALKPKRGLGIDFSPAMIRQAVARHPETEFRATDVDDLDVR
                     ETFDVILLLDVIGHLLDVESTLKRLRRFCHPQTRVIVVYYNFLWEPFLRLAERWGLKM
                     SQQQQNWLSPADVRNLLRLADYEVVKAENRFLLPKKIPLIATFLNRFAVILPGINRLC
                     LAQYVVARVRGMQVAQAYSVSIVIPCRNEMGNIEDAIIRLPAFGTHQEIIFVDGHSTD
                     GTVQEIERIIKKYPQKDIKLFIQDGKGKGDAVRKGFAHAIGEILMILDADLTMPPEDL
                     PKFYNALANDKGEFINGSRLVYPMEQKAMRLLNLLGNKFFSMVFSWLLGERIKDTLCG
                     TKVLFRRDFDKISANRAYFGDFDPFGDFDLLFGASKLNLKILEVPIRYQERTYGTTNI
                     NRFQHGWLLLKMTFYGFFRLKAV"
     gene            complement(3431..3967)
                     /locus_tag="VFR82_12535"
     CDS             complement(3431..3967)
                     /locus_tag="VFR82_12535"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HEU4502470.1"
                     /translation="MSAEPTTSQTSDSQSASHWYALRTRSRHEKMVRDQLVHRGIEHL
                     LPTVKRLSQWKDRKKEVEVPLFSGYCFVRFGSDQKLPILKTVGVVDIVGSGQRPEPIP
                     DEEITAIQTLMASVLPYDPHPYLQEGMLVEVIRGPLQGIRGMLLRKDKRHRLVLGVRL
                     IQQAAAVEIDTGDVVAVG"
     gene            complement(4021..4608)
                     /locus_tag="VFR82_12540"
     CDS             complement(4021..4608)
                     /locus_tag="VFR82_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU4502471.1"
                     /translation="MDLQGQRDLVLLSELDRNGGATQRTLATKLGVALGLTNLYLKRL
                     ARKGYIKITTIQRNRIRYLLTSQGFAEKSRLTYQYMQYSLSYYRDMRGRLKEMLSTLE
                     WTHGQRVAICGTGELAELAYLALREMNVNCVGFVDGNTRESFLSCPVSSPDGIVGWQL
                     DRVLIADLDSAETYEEQLVRSGIPREKLLRLGLRH"
     gene            complement(4681..5577)
                     /locus_tag="VFR82_12545"
     CDS             complement(4681..5577)
                     /locus_tag="VFR82_12545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEU4502472.1"
                     /translation="MPKVTVVIPIYNGSRYLRESIDSVLAQAFHDYEIVCVDDGSTDD
                     SAVLLQEYGQRIRVVRQENSGQSAARNAGVKLARGEYIAFLDQDDVWYPSKVQSQVAV
                     LDAEPDVVLVHCNFDRIDERGQMLQRGAGMIERTSALGSSMGQLIGEALIFPSAMMIR
                     KGCYERIGGFHHALQGFEDFDLIARLKEQGRFVMVEERGMAYRMHGSGFTRTGGIHVI
                     RSRETFLRRMQALYRGHWTKQAIVKNMLADCYSDWGIHDVRSGNRQEGRVRLIQSIGC
                     NPFKFRTYTRLLRAFLPPGRMG"
     gene            complement(5585..>6086)
                     /locus_tag="VFR82_12550"
     CDS             complement(5585..>6086)
                     /locus_tag="VFR82_12550"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4502473.1"
                     /translation="ILDIGCGANKHDGALGVDCRKVPGVAVICDFEQGLPFREGSVTV
                     IYLHHIVEHMHDLIAFMVELYRVCEPGAKVFVRTPYYASREAFVDPTHVRYITEATFE
                     YFDYPNYYGLNTNIKIRSIDYKMRKSLRFLPRYFQKRCRHFLWNACIEMDLVLEVIKP
                     LSPRSE"
BASE COUNT         1572 a         1675 c         1491 g         1348 t
ORIGIN      
        1 tgcaaacatg taatcgaacg tattgcgaac gacgaaacca agcacagaca atgcgatagc
       61 tgctggaagc aaaagcattc ctgcatgctg aaaggagagt tccctgatac cgcttatcaa
      121 tttccgaatc gcacgaacga ttgtccacac gaacaacaac aaagcgggca caccgctacc
      181 taggagtacc atcgcaaagg tgttgtgcaa cgctggtaaa acttttgcta ccggtccttt
      241 gctcttatcc gcttcaattt cagcggtgta cactttggaa aacgtatcat tcccaaagcc
      301 gatgccaacg agagggtgtt cgaccacttg ctcaaatccg agatgccacg ttttcacacg
      361 tgcgctcaag gtccacggat caagagtctc ttgctgatag ccgcgagaag ctaaggtaaa
      421 caacgcagcg cttcccgcca ggattgcacc aacgagaaac catacgagcc attgttgccg
      481 gtgaatgatg agcacgaaac ccaatgcctg gacgaaatgc gcgacccagc cggctcgggt
      541 ataggctgca atctgcgcaa gcagcgatgt ggctagacta caaagtccca gcgctctcgg
      601 cgccagactc gactcgatca gtagtcctcc aattaagaca ggaatgacaa gtacgagata
      661 ggtggtcaac caattgtagt cggaatacgg cgcggcagcg cgaactagac ggtctctcca
      721 tgtacctcca cgtgacagaa aatcttcgag ggcaaagagt gacaacacaa agcttcccaa
      781 gacgattgcc aacaacactt tgcgccctgg ctcgacatcg ccgtgtaagc gcaagacgaa
      841 catggcccag taaaacacga gcatgtgcgc aacaaacttt cgccattcag aaaaactata
      901 ggcaggatca atcgaaaacg gaaccgtgaa caacacccaa ctgataaacg caaataacgg
      961 cagatcaaga cgcgttcgaa cataaggatt gaacttttcc actcgggcta aggccaacgc
     1021 cagggcgaac aacaagaaga aggcatattc ttgaaaatgg aaaaggcttg gaaagaaact
     1081 cagaaaagta attccgcata gcccgtatat ttgaagttgt aaaataatcg gcttggcacg
     1141 aaagccatgt cgaaaaagtt tgctgttctc tgcggacgcg tttccgtcct cgctctcaag
     1201 tttcatgatg ggagaatgaa acacttatcg acatcatgag gcggtagaga tcagacggat
     1261 ttctccaaca ccacaagcaa ccggaaggcg aacaggcgcc cgaaatagcg gcaccactgc
     1321 tcaagccgca acaccatgtc gatggaccat agagggagga atgatcgacg atcgaacccc
     1381 ccgctgaggg gataggcaaa gtaggccagc cgttcgtgcg ccagtttgac gaaatgcggg
     1441 tagagttgat gaaaccggtc ataggatcgc tcaaaaatga tgcgggcaat ggcttgattg
     1501 gcatcgaaag gttgtcgctg aactgtgggg atgatggtaa gcggatcgtg gctgaaatcc
     1561 atcggttcag gatgaaagaa cctatagaca ggccacgaga gcacggagac gtagggatcc
     1621 atgatgacaa tgcgcccaga tggaattaac acacgaagcg cctcgtcaaa aaaatagcgc
     1681 acgttttcca agtggtgcag cacgtcgaat aagacgatgt tggacagact tctgcttcga
     1741 aatggcaggt gctgcgcatc cgccagggca tcgagccatg gcatcctgac aacatctgtg
     1801 caaagcacat ggggggagaa ttcctttaaa ttccctgttc ctccgccaac ttcgagggtc
     1861 acaccgggtt gaagccatcc tgtaatctgt cgataccacg acgcgtacaa ccgcctgaga
     1921 attggcttct gcttccaaac cgcctgatgt cgacgcaaga tctcttctga cattcacact
     1981 gctttcaggc ggaagaaccc atagaaggtc attttcagca gcaaccaccc gtgctggaac
     2041 cgattgatgt tcgtcgtacc gtaggtccgc tcctgatagc ggatcggcac ttcaagaatt
     2101 ttcagattca gcttcgacgc gccgaataaa agatcgaaat cgccgaacgg atcgaaatcg
     2161 ccaaaatacg ctcggttcgc agaaatttta tcaaaatccc ttcgaaacaa cacctttgtt
     2221 ccacataacg tgtccttgat tctctcaccc agcaaccatg aaaacaccat actgaagaat
     2281 ttgtttccga gaagattcag gaggcgcata gccttctgct ccatgggata caccaaacgg
     2341 ctgccattga tgaattcgcc cttgtcattg gcaagagcgt tatagaactt agggagatct
     2401 tccggaggca tcgtgagatc tgcatccaga atcatgagga tctcaccgat tgcatgggcg
     2461 aatccttttc gaaccgcatc cccttttccc ttaccatcct ggatgaacaa cttaatgtcc
     2521 ttctgggggt actttttgat gattcgttct atttcctgga cggttccatc ggtggagtga
     2581 ccgtcgacaa agataatttc ctgatgagtt ccgaaagcgg ggagccggat aattgcatcc
     2641 tcgatgttcc ccatttcatt acggcaaggg atgacgatgc tgacagagta cgcctgggcc
     2701 acctgcattc cccgaaccct cgccaccaca tactgtgcca ggcagagccg gttgatgcct
     2761 ggaagaatga ctgcaaatcg gttcaagaac gtggcgatga gcggaatttt ctttggcaat
     2821 aggaaccgat tctcggcctt cacaacttca taatcggcca gacgcaacaa atttctgaca
     2881 tcagccggcg aaagccaatt ctgttgttgt tgagacatct tgaggcccca tcgctctgcc
     2941 agacgcagaa acggttccca taaaaaattg tagtacacga caatgactct ggtctgcgga
     3001 tgacaaaatc gacgaagcct tttgagcgtg gattcgacat cgaggaggtg tccgatgaca
     3061 tcaagcaata agatgacgtc gaaggtttct ctcacatcca aatcatccac atccgttgcg
     3121 cggaattcag tttcgggatg gcgggcaacc gcctggcgaa tcatcgccgg actgaagtca
     3181 atgccgagcc cgcgcttagg cttcaacgca gccagttgat cgcccagccc gcaaccgacc
     3241 tccaaaatgc tcagtccttc ggggacgaga aagcggagat atcgcagttg atcctcgtag
     3301 taataccggg ccttctcctg ccaccgcgta cgctgcgagg ataaccgatc gaaacgttcg
     3361 gcaatctcat gcttacgccg ttgatcgcgt tccatgaaag ctagcccgct tcttgacatg
     3421 cttccgcccg ctatccaaca gccacgacat cgcccgtgtc gatctcgacg gcggccgcct
     3481 gttgaatcag ccgcactccc agcaccaatc gatgacgctt atctttgcgg agcagcatcc
     3541 ctcgtatgcc ttgaagcggc cccctgatga cttcgaccag catgccttcc tgcaaatacg
     3601 gatgtggatc ataaggcaag acgctggcca tcagcgtctg gatggcggtg atctcctcat
     3661 ctggaattgg ttcggggcgt tgtccactgc ccacaatgtc gaccacgcca actgttttga
     3721 gaatcggcag cttctggtcc gatccgaatc ggacgaagca gtatccggag aacaacggca
     3781 cttcgacttc tttcttgcgg tccttccatt gactcagacg cttgacagtc ggcaagagat
     3841 gctcgatacc tcgatggacg agctgatcgc gcaccatttt ttcatgccgc gatcgcgtcc
     3901 ggagcgcata ccaatgggaa gcgctctgag aatcgcttgt ctgactcgtt gtcggctcag
     3961 cggacatagc ttcgccctct cacttacagt gcgttctcag caactgcctg gcctacggct
     4021 ctagtgacgc aggcccagtc tcagcagttt ctcgcgaggg atcccggacc gaaccaattg
     4081 ctcttcgtaa gtctccgcag agtcgagatc ggcaatcagc actcgatcca attgccagcc
     4141 gacaatccca tccggcgaag acactggaca cgacaagaag ctctcccgtg tgttgccgtc
     4201 aacaaatccg acacaattca cattcatctc ccggagcgcc agatacgcca actctgccag
     4261 ctcccctgtg ccgcagattg ccacacgctg tccatgggtc cattccaacg ttgacagcat
     4321 ctctttaaga cgcccacgca tgtcgcggta ataagacagc gaatactgca tgtactgata
     4381 ggtcagacgg gatttttctg cgaatccttg ggaagtgagc aagtatcgga tccgattccg
     4441 ctgaatggtc gtgatcttga tgtagccttt gcgagccaag cgcttcagat acagattggt
     4501 caaaccaagg gcgaccccaa gtttggtcgc aagggtgcgc tgcgtcgctc ccccgttccg
     4561 atcgagttcg ctcagcagta cgagatcgcg ttggccttgt aaatccatga attgtgcagg
     4621 taattcggcg aagcgttcac aatatgaact aaactggttt gcctagtcaa gggaaagaga
     4681 ctagcccatg cgacccggag ggaggaatgc gcggagcaat cgggtgtacg tccgaaattt
     4741 gaaagggtta cacccgattg attgaatgag ccttacccgt ccttcttgcc tgtttccgct
     4801 tcggacatca tgtatgcccc agtcggaata acaatctgcc agcatgtttt tgacgatggc
     4861 ctgcttggtc caatgtcccc gatacagtgc ttgcatgcgg cgaagaaacg tctcccggct
     4921 acgtatgacg tgaatacctc ctgttcgtgt gaacccgctt ccatgcattc tgtaggccat
     4981 gcctcgttct tccaccatga cgaatcgtcc ttgttctttt agcctggcaa tgagatcgaa
     5041 gtcttcaaat ccttgcaggg cgtgatggaa ccccccgatt cgctcataac accctttgcg
     5101 aatcatcatg gccgatggaa aaatgagtgc ttcgccgatc agttgtccca tggaagaacc
     5161 cagcgctgac gtccgttcga tcatccccgc tcctcgctga agcatctggc ctctctcatc
     5221 tatccggtca aagttacaat gaaccaacac cacatcgggc tccgcgtcca gcacagccac
     5281 ctgactctgg accttggatg gataccacac atcgtcctga tcgaggaatg cgatgtactc
     5341 accacgcgcc agctttacgc cggcatttcg tgctgcactc tgacccgagt tctcttgacg
     5401 caccactcga atccgctggc catattcttg gagcaggaca gccgaatcat ctgtggagcc
     5461 atcgtccacg cagacaattt cataatcatg gaatgcttga gctaagaccg agtctatcga
     5521 ttcgcggaga tatcggctcc cgttgtagat cggtatgacg acagtcacct tgggcattgg
     5581 aggctcactc gcttcgtgga cttaggggct taatgacttc gagcacgagg tccatttcta
     5641 tgcaggcgtt ccacaaaaaa tgccgacaac gcttttgaaa gtagcgtggc aagaatctca
     5701 aggacttccg catcttatag tcaatggagc ggatcttaat gttcgtattg agaccgtaat
     5761 aattggggta gtcaaaatat tcgaaggtcg cctcggtgat ataccggacg tgcgtcggat
     5821 caacgaaagc ttcccgcgaa gcgtaatacg gcgtccgcac aaacaccttg gcgcccggct
     5881 cacacacccg gtacagttca accataaagg caatcaaatc atgcatatgc tcaacaatgt
     5941 gatgtagata tatgactgtt acgctcccct ctcggaaggg cagtccctgc tcaaaatcgc
     6001 aaatgacagc aaccccggga accttccggc aatcgacacc aagtgctcca tcatgtttgt
     6061 tcgctccaca accgatgtca agaatc
//
LOCUS       DASTQY010000387        18371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_887073, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000387 DASTQY010000000
VERSION     DASTQY010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 18371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18371
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887073"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..215
                     /locus_tag="VFR82_12555"
     CDS             27..215
                     /locus_tag="VFR82_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502474.1"
                     /translation="MIKMWERVSAVAGTFVIVGASSVWANEPKGGAPDYSGISTMYYA
                     LIGLVLAYGVYDTFFKKS"
     gene            complement(290..952)
                     /locus_tag="VFR82_12560"
     CDS             complement(290..952)
                     /locus_tag="VFR82_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEU4502475.1"
                     /translation="MTLRTLGQVFAASFITLLLATTLPPIAHSAGADNNNDRPRIVAF
                     GDSLTAGLGVAAEETYPAQLQRRLDSRGLQYRVINAGVSGDTTAGGLRRVDWVLKSRP
                     NVVILELGANDGLRGLKLQETKTNLEKIIQRCRDASVLVVLAGMKLPPNYGTEYTKSF
                     ESIYPALAKQFRLPLIPFFLEGVAGSTSLNQQDGIHPTSEGYGIIADLVMETVTPLLV
                     RQ"
     gene            1008..1703
                     /locus_tag="VFR82_12565"
     CDS             1008..1703
                     /locus_tag="VFR82_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4502476.1"
                     /translation="MIAVRHLSMRLTAGGRPVTILDDVTLSIPPKQMVAVMGPSGSGK
                     STLLGLIAGLDRPTEGTIILDGSDITTLSESRMARFRRIKIGYIFQSFHLIPTLTAFE
                     NVLVPLELAGDAAAKAVAIELLHAVGLQDRVHHYPVQLSGGEQQRVAVARAFACRPPI
                     LLADEPTGNLDSQTGRHVMDLLLSLHRDYGTTLVLVTHDRAIASLMHRVIALRDGRIE
                     SDDLISERQESPL"
     gene            1700..4357
                     /locus_tag="VFR82_12570"
     CDS             1700..4357
                     /locus_tag="VFR82_12570"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU4502477.1"
                     /translation="MTRFMFSMAWRETRGAWRHFVYFLVCIAVGVSALVGVSLFATHV
                     DNAVSKQAKGLLGGDLEIRSSHRLTEESRAIVDSLHQRAVDTTHVTELVAMAARPDTA
                     GSGHTTQIVELKAVDPGYPLYGVLKVEPALPLERLLEPDSTACGRCQGVLVQEALLLR
                     MGIRVGDRLKLGKAEFLITGLVRTEPDRMANAFSLGPRVIVSQAGLQSAGLVQRGSRV
                     RERQLLRLPSGMPLEPLRAELRGRLAADHVRISGYRDAQPQLKQFLEQLARYLGLIGL
                     TALFIGGLGVAMSIHAFMQEKMKTIAILKTLGADSAALIKVYIVQAIGLGLIGSGVGI
                     VLGILLQRWLPPLLAGAFVSDLLDQLGVSAELSLVSIGPLLKGGAMGLLTTVLFALWP
                     LLRVREIRPAAIFRHEVEGGVADEQNRRQTWRIRWGLIDKAHVVIGGSILAGLGALSI
                     WQAGSWKVGGLFIGGLAVAMLTLFLCARVLIRALRMVPRPASLSLRYAVGNLLRPGGH
                     TAGVLVAIGISVMVIMTVSLVEHALLRQVGDNRPVDAPTFFFIDLQVDQTEGFAKLVQ
                     QLTAGRRPEMTPLVRSRLHTVNGEVVKVEEERDQEERRDENKEERRKQWYLSREYVLT
                     FLDQLPKGNEVVAGTWWQPGQRFSAPQVSIEEEAARSLGLVVGSQLELDIQGATIAAE
                     VSSIRKVDWGNFSTNFYMIMSPGSLDGAPLTYVATIHVTPEQEVPLQQAVVAAFPNVS
                     AIHVGDVLRNFASVLDRLALAIRAVALFCVMTGGLVLAAALAATRYRRLYESVVLKAL
                     GATRSLIARIFALEYALLGVVGGTLAAGLSSALSWLVLTYVFDLSWRLHPMVLLTGLL
                     STMVLTLAVGILSTFRILGQRPLAILRHE"
     gene            complement(4401..5594)
                     /locus_tag="VFR82_12575"
     CDS             complement(4401..5594)
                     /locus_tag="VFR82_12575"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU4502478.1"
                     /translation="MPSSAGSIGIFGVMLCALVVACNGSDDVGGGGASPPPATSNGLR
                     LQTVTAVLSSPVFLTAPAGDVARLFIVEQGGLIRILNSLDGTPRATPFLDVSGLVVTG
                     GEQGLLGMAFDPNYAGNGRFYIHYTNTAGDIVIARYGVSSNPDIANAGAQAILKTIAH
                     PTNQNHNGGMLAFGPDGCLYAGIGDGGGIGDPNGNAQNTNSLLGKILRLDPETGSACG
                     TDNPFANGTGGAPEVWSFGLRNPWRFSFDRSTGVLYIGDVGQSQREEVDAVAGPNAGR
                     GVNFGWNIMEGFACFNPPSGCNSNGLTPPILDYSHDAGACSVTGGYVYRGTLNPAVNG
                     SYFYADFCAGFVRSFQLQGGRPSSQNTWPLLSPGGQITSFGEDARGELYIMTQTGGLS
                     RIVAN"
     gene            complement(5607..8231)
                     /locus_tag="VFR82_12580"
     CDS             complement(5607..8231)
                     /locus_tag="VFR82_12580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU4502479.1"
                     /translation="MNEVPYGHDDPYRLPQHVRPTRYQLTFEPDLAGATFLGRATIVL
                     TVLQQTRVIVCNALELAIDEAIVEAVKGRRQTASVTLDESLQRCQLTVAEPLAPGTWQ
                     LHISFRGTLNDKLRGFYRSTYTDQRGSQQTIAATQFEATDARRAFPCWDEPDFKAVFS
                     TTLVIDESLTAISNSLVLSEAREKGKKIVRFADTIKMSTYLVAFVVGRMEASAPSVVG
                     KTRLQLWTVPGKTHLTGFGQDIAAASLQFFERYYGIPYPGDKLDLLAIPDFASGAMEN
                     LGAITFRETALLVDQRTGTHGELQRVADVVAHENAHMWFGDLVTMSWWNGLWLNEAFA
                     TFMEMLAVDAWKPEWKRWDSFGASRGAAFAVDGLHSTRPIEFPVRAPKDAEAMFDVLT
                     YEKGASVLRMLEQHIGPTVFRDGVREYLRTHAYGNADTNDLWVSIGKASNQAVPDLMN
                     GWIFQPGYPLVTAELTKQCELVLSQQRFSYLPDPPPATWQVPVQLRVQTGAEGEVRRV
                     LLTGTRLNVSCPPETQFILVNEGGHGFYRVRYGPNLLKRLLEAGLEKLAPIERLNLVN
                     DAWATTVAGLMPLCDYLDLTGRFSLERDKNVWAVLIDSFAFLNRIIEPADRAGLESFV
                     RSRVGEAAAAVGWTTRPGENDLTRQLRGELIGALGRLGNDPATQARANELYATARKDP
                     ATIDPNLLPALVSILAFIGDAARYEDFRTRFKTAATPQEERRYLFSLAMFQQTALLDR
                     TLNMTLNGEIRLQDAPFMVSAVMSTVYGREQGWRFVKTNWDLLDRRFPKQGLRRMCGG
                     ITSFCKPELERDVREFFQARQIDLGGKTLEQYLEQLRIGVSFGERSRRELQEFLRNNQ
                     YSTHVAQTSFPVEPTEVS"
     gene            complement(8374..9072)
                     /gene="rpiA"
                     /locus_tag="VFR82_12585"
     CDS             complement(8374..9072)
                     /gene="rpiA"
                     /locus_tag="VFR82_12585"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU4502480.1"
                     /translation="MSTSDLDTLKHAAAVKAVEFVKDGMVLGLGTGSTAKHLIMALGE
                     KVKAGVNLRGVSTSQATAMLARQCGIALIDAETCWKIDVAFDGADQVDPAFNLIKGGG
                     GALLKEKIVAASAMKFVVMVDFTKRVPVLGGDFPLPVEVVPFGWRSTARKIEELTRSR
                     VVLREREGARFQTESGNLIVDVHLARIDRPDELETALHRIPGVVEAGLFVGRTDVLIV
                     GTPDRTEIHHVKRG"
     gene            complement(9069..10124)
                     /gene="glk"
                     /locus_tag="VFR82_12590"
     CDS             complement(9069..10124)
                     /gene="glk"
                     /locus_tag="VFR82_12590"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEU4502481.1"
                     /translation="MILAGDIGGTKTHLALFDWTTDRVDPLRLETFHSADYASLEDML
                     EEFLAPPKPPTPIDHLEPEPQDKPLSSPQDELPKIRAACFGVAGPVIQNHSRTTNLPW
                     VVDGAALAKRFELPAVQLLNDLEAMAHGIVLLRADELVTLNQGTPPMQKQAIALIAAG
                     TGLGESILFWDGTRYKPIPSEGGHADFAPNSDHEIDLLRYIRSQYLHVSYERVLSGPG
                     LHAIYEFLRDAKRNEPTWLAEKIKAGDPAAEIADAGLKGQAEIAKQALDLFASIYGAE
                     AGNLALKAMAINGVYVGGGIAPKLLAKLKDGTFMKAFTNKGRYKRLMGTIPVHVVMNQ
                     HTGLLGAASTAVRLAQA"
     gene            complement(10121..10900)
                     /gene="pgl"
                     /locus_tag="VFR82_12595"
     CDS             complement(10121..10900)
                     /gene="pgl"
                     /locus_tag="VFR82_12595"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEU4502482.1"
                     /translation="MSRAEIRVAQDSSKWATEAASLTQELHRQAVKERGLFLIALSGG
                     RTPEQLYRRLASAPFCDQDDWNNTQFFFSDERCVPVHHPDSNFDLADETLFRPLNIPS
                     HHIHRMACEQADADVAAHNYEGLLRAVTKTPPERWPRLDLVLLGIGQDGHTASLFPGT
                     DAVQERRRCVTVGYAPSGPRTRLTLTLGVINQATVVLFLVTGAAKAGIVKTILEPQQE
                     TDRLLPAALVRPDGGRLIWLLDPPAAAQLTGHRESNSPGQI"
     gene            complement(11125..11781)
                     /locus_tag="VFR82_12600"
     CDS             complement(11125..11781)
                     /locus_tag="VFR82_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502483.1"
                     /translation="MPRPSVTWILIWSFLLMAASPTQAADAEYPLSVDVTNPAALQEA
                     TRVLAEEVKLASRPQTYVIVDLVDRAVLIKGRGIELHRFPIEQWSGGQLADASATFRL
                     QTRPPVTRRKIEPAAGGEQPPISLDDMPTDFTLQFSPTFSLTVHPSASDDIGRWLRFK
                     GREWWMWLKDWAITLTTGKGPLPQPWLYLSVTSDHAQSLAWTVTEGMAFLIRRTPPSS
                     "
     gene            complement(11786..12418)
                     /locus_tag="VFR82_12605"
     CDS             complement(11786..12418)
                     /locus_tag="VFR82_12605"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEU4502484.1"
                     /translation="MTRIVASLCLGLIGLVLLTLAQSSSLPVTDASTIPAVVPDPATL
                     HVLQNRYRSLSKQLNQLMPSQPYIVVDTARNHLYIKRQDHVMLDALASTGSGTILDKP
                     GEAKGQWVFDTPRGEFTVQSKLINPVWVKPDWAFVEEGLAIPKSYTDRIEPGVLGEYA
                     LGFGKGFFIHGTLYTRLLGKNVTHGCIRLNDDDLKSVYRLAKVGTPIMIF"
     gene            complement(12425..13576)
                     /locus_tag="VFR82_12610"
     CDS             complement(12425..13576)
                     /locus_tag="VFR82_12610"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEU4502485.1"
                     /translation="MREMSLRYAVRIVCYSSLFALCGSLPACVESVPPDVVQAIESVD
                     KDLAQLRAAELAPSAYNAFAYQWVTLRSRVEAEEDTVRWPWEPNDLEEALRGLHAEGT
                     RTVALLMEQQEMLRRSAARQLAEVEEQAHLLATHVGAIDSRLILGEKPVETDLLLKQA
                     RTFYEQRRYEQSLSASRRASHYLAAQSALLQRELGRYASPDRVARWQRMASETIEWSR
                     NHHRTAIVVSKAERSLTLYRNGQKVVSYPVRLGFNGIKEKRYQGDGATPEGRYRVLSK
                     RGRGQTQFYRALLLDYPNQDDRRRFLSDRKMGNIPTLRGIGGQIEIHGVENELMAQTL
                     GCVMLENAQMAALFDRVDAGTPVTIVGALLEQNSVAKALASLSVHRNEI"
     gene            complement(13582..14196)
                     /locus_tag="VFR82_12615"
     CDS             complement(13582..14196)
                     /locus_tag="VFR82_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502486.1"
                     /translation="MKMRHFLVAVLSVWFLAAGCAQPPADQINAAEQAVKDAQQSGAA
                     TYAANDHAKVEGLLAAMKQEVADQDAKFALLRDYGKAEQLAATTKAEAERVKADATKK
                     MEEAKFAATQAQHGAEAAVQSTLELVSRAPAGKDRAALESIKTDAQALKASLNDVQLM
                     FDKSDYLGAQTKAKAIQEKAEAVSHEIQTALAKIGKGKPVTKKK"
     gene            14585..14722
                     /gene="rpmH"
                     /locus_tag="VFR82_12620"
     CDS             14585..14722
                     /gene="rpmH"
                     /locus_tag="VFR82_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU4502487.1"
                     /translation="MSFTFQQPSNLKKKREHGFRKRMSTKNGRKVLARRRAKGRARLT
                     V"
     gene            complement(14878..15123)
                     /locus_tag="VFR82_12625"
     CDS             complement(14878..15123)
                     /locus_tag="VFR82_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502488.1"
                     /translation="MIGSQLVSNLSRQLPEQTFDSIATNGNSEPSPHNDADAARAAFG
                     LAHQQVEAGCGQPPAMLFHKFNVAARAKKKDSISCPP"
     gene            15239..15478
                     /gene="yidD"
                     /locus_tag="VFR82_12630"
     CDS             15239..15478
                     /gene="yidD"
                     /locus_tag="VFR82_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU4502489.1"
                     /translation="MKTMCVALINGYRFWISPWLGPACRFDPTCSDYAGQAIERYGVI
                     RGASLAAWRLLKCHPFHPGGHDPLMPEVTNRGTAE"
     gene            15480..17144
                     /gene="yidC"
                     /locus_tag="VFR82_12635"
     CDS             15480..17144
                     /gene="yidC"
                     /locus_tag="VFR82_12635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU4502490.1"
                     /translation="MEKRVVIFLVLSLAVILGYDLLLKQLGLLPTTPTVQEPGGQEAT
                     APRGQTERPSAETTPSTPRSLSPASDTASAGDSRTSTPATERTVTVETDFIRVAFNAR
                     GGVISSWELKRHYTSATDGNPVQLVYQGAKFKEPLSITTSDAAADKALREGLYAIEAD
                     FTTLDDAHPTGHVTLRFHDPALGLNVQKRLTFRQENYLVDVSLTVDGLSGGYDIVLGT
                     NFGIVEWGDGFIGLIGSASLVDGKVEKETPDLEFERKGSVQWVALQDKYFLSVLMPQG
                     ASAALAKKEGDKLVSAGVRMPASGARTTDLQLYAGPKEYDTLRSLNVGLEETIDFGWF
                     IFGSWSVVKAVAKPIFYVLRFINEYTHNYGVTIILLTMGIKMLFVPLQYKSYKSMKQM
                     QLIQPKVLALQEKHKDDRDKLNKELIKLYRDHKVNPVGGCLPMVLQMPVFVALFNILY
                     MTIDLRQAPFVGWITDLSVQDPYYVLPIIMGATMVIQQKITPTTMDPTQAKIMLVLPV
                     FMTFLFVNFPAGLVLYWLTNNVLTIGQQVLTDRLFGSRPFVTDPAK"
     gene            17157..>18371
                     /gene="mnmE"
                     /locus_tag="VFR82_12640"
     CDS             17157..>18371
                     /gene="mnmE"
                     /locus_tag="VFR82_12640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU4502491.1"
                     /translation="MQRGGPDDTICAIATPAGEGGIGIVRLSGPQALRIADKIVQLRS
                     GQPLASVASHTLCLADLRFPDMNQTVVSPDPSLPKPRECFDEALVVYMKAPRSFTGED
                     IVEIQSHGGSLILSLTCRACLERGARLAEPGEFTKRAFLNGRLDLSQAEAVLDTIRAK
                     SAASLTAAQRQLRGDLAREVDTAREALVHLLAQLEAGIDFSDEDVDIIQKQDLLETID
                     RAAMLVERLRATARTGRLLRDGARVVIAGRPNVGKSSLLNRLLKEERAIVTAVPGTTR
                     DLIEEAIDLGGVLVHLVDTAGIRDTEDPVEHEGIKRSWSAQEQADLRVIVLDGSLPLT
                     ADDRRLMDQAAEMRHVIVINKADLPAQVSPTSVNPGSVCIAVSAKTGDGVEDLRLAIR
                     AQLTGSGPETADG"
BASE COUNT         3746 a         5170 c         5431 g         4024 t
ORIGIN      
        1 gtcattaaca tcgagggagg atacatatga tcaagatgtg ggaacgggtc agtgcagttg
       61 ccggaacgtt cgtgatcgtt ggcgcttctt ccgtgtgggc caacgagccg aagggtgggg
      121 cgccggacta tagcggcatc agcaccatgt actacgcact catcggattg gtactcgcct
      181 acggcgtgta cgacacattc ttcaagaaga gctgaccttc tgtcttttgc cccgggctca
      241 gcggtgttga gtgagcccgg gcgatgctct ccagtcaaaa ctctccttct tattgccgta
      301 ccagcagagg cgtgaccgtc tccatgacca agtctgcaat gatgccataa ccttcgctcg
      361 tcgggtgtat accgtcctgt tgattcaatg aagtagaccc cgccaccccc tccagaaaga
      421 agggaatcag cggcaatcgg aactgttttg ccaaggcggg atagatggac tcgaaacttt
      481 ttgtatattc cgtgccatag ttcggtggca gtttcatgcc ggcgagcacg acgagtacag
      541 atgcatctcg gcaccgttgg ataatcttct caagattcgt cttagtttct tgcagcttga
      601 ggccacgaag cccgtcgttg gctccgagtt ccaaaatgac cacgttcggc cggcttttca
      661 acacccaatc tactcgccgc aggccacctg ccgtggtgtc cccgctcact cccgcgttga
      721 tcacgcgata ctggaggcct cgggagtcaa gccgacgctg taactgggca gggtaggttt
      781 cttctgccgc cacgccaagg ccggccgtca agctatcgcc aaaggcgaca atgcgcggac
      841 ggtcgttgtt gttatccgca cctgcagagt gtgcgatagg gggcaacgtt gtggcaagca
      901 acagagtgat gaaacttgcc gcgaatacct ggcctaatgt tcggagtgtc attggactcc
      961 agtataatat catctagtcg tctcactgag gaacgaggta gagggatatg atcgccgtcc
     1021 gacatctttc catgcgactc acagccggtg gacgaccggt gacgatactc gacgacgtga
     1081 ccctgtcgat tcctccgaag caaatggttg cggtgatggg accatcaggg agcggaaaat
     1141 cgaccttgct cggactgatc gcgggcttgg atcgaccgac cgaaggaacc atcatcctgg
     1201 acggaagcga tatcaccact ctctcggaaa gccggatggc ccggttcagg cgcataaaga
     1261 tcggatacat ttttcaatcc tttcacctca ttcccacatt gacagccttc gaaaacgtgc
     1321 tcgttccctt ggagctcgca ggtgatgctg ctgcgaaagc tgtggcgatt gagctcttgc
     1381 atgccgtggg cttgcaggac cgagtgcatc attatcctgt gcagctttcc ggcggggagc
     1441 agcaacgggt tgccgtagcc agagcgtttg cctgtcgccc gccgatcctt cttgccgacg
     1501 aacccaccgg gaacttggat tcgcaaaccg ggcgccacgt catggatctg ttgctttctc
     1561 ttcatcggga ttacggaacc acactggtgc tcgtgacgca tgacagggcg atcgcgtcct
     1621 tgatgcaccg ggtgatcgca ctgcgcgatg ggcgcatcga gtccgatgac ctcatatccg
     1681 agagacagga gtctccgttg tgacccggtt catgttctcg atggcctggc gggaaactcg
     1741 tggggcgtgg cgtcattttg tgtattttct cgtctgcatt gcggtgggag tgagcgcgtt
     1801 ggtcggcgtc tccctcttcg cgacccacgt tgataatgcg gtctctaagc aggcaaaagg
     1861 cctccttgga ggggacttgg aaatccgctc ctcacatcgg ttgacggagg agagccgggc
     1921 gatcgtcgac tctctacatc agcgggcagt tgatacgacc catgtcaccg aactggtggc
     1981 catggcggct cgtccagata cggcagggtc cggacacacc actcagatcg tggaactcaa
     2041 ggcggtcgat ccaggctatc ccttgtacgg cgtattgaaa gtagagcctg ccctcccgct
     2101 ggagcgcctg ctcgaaccgg attctacggc atgcggacgc tgtcaaggcg ttctcgttca
     2161 ggaggccttg ttgcttcgca tggggatacg ggtcggcgat cgattgaagc tcgggaaagc
     2221 agaatttctc attacggggc tcgtgcgaac tgagccggat cggatggcca atgccttcag
     2281 tctgggtcct cgggtcatcg tctcgcaggc tgggctgcag tctgctgggc tcgtgcaacg
     2341 gggaagccgc gtccgtgagc ggcagcttct acggttgccg tccggaatgc cgctggaacc
     2401 tctgcgggct gaattgcgcg gccgcctggc ggcagaccat gtgcgcatct ccggctatcg
     2461 cgacgcacaa ccccaactca agcagttcct cgagcaactg gcgcgctatc ttggcttgat
     2521 tggtttgacg gccttgttca tcggtggcct gggtgtcgcg atgtcaattc atgctttcat
     2581 gcaggagaag atgaagacga tcgccattct gaagacgctg ggggcagatt ccgccgcgct
     2641 gatcaaagtg tatatcgtgc aagccatcgg cttgggactc atcggcagtg gagttggcat
     2701 agtgctcgga atcctattac agcgctggct ccctccactg ctcgccggtg ccttcgtcag
     2761 cgacctcctt gatcagttag gagtctcggc cgagctttct ttggtgtcca tcgggcctct
     2821 cttgaaaggc ggtgccatgg gcctgctgac cacggtactc ttcgcactgt ggcccttact
     2881 acgtgtgcgt gagatcaggc ctgctgccat ttttcggcat gaagtggagg gaggtgtcgc
     2941 ggacgaacag aaccgccgcc agacgtggag gattcgatgg ggcttaattg acaaggctca
     3001 cgtggtgatt ggggggtcca ttttagccgg cctcggagcg ctctcgatct ggcaagccgg
     3061 ttcgtggaaa gtcggcggat tgttcatcgg cggattggct gtagccatgt tgacgctctt
     3121 tctctgcgca cgggtgctga tcagggcatt gcgcatggta ccgagaccag cgtctctgag
     3181 cttacgctat gccgtcggca atctgttacg acccggtgga catacagctg gcgtgctcgt
     3241 ggccatcgga atcagcgtga tggtgatcat gacggtttcc ttggtggaac acgctttgct
     3301 tcgacaggtg ggagacaatc gtccggtgga tgctccgacg ttcttcttta ttgatttgca
     3361 ggtggatcag acggaaggtt ttgccaagct cgtccagcaa ttgaccgcag gacgccgacc
     3421 tgaaatgacc ccgctggtgc ggtcccgcct tcatactgtc aacggcgagg tcgtaaaggt
     3481 tgaggaggag cgggatcagg aggaacgacg tgatgagaac aaggaggagc gtcggaagca
     3541 atggtatctc tctcgcgaat acgtgctcac gttccttgat cagcttccca aaggcaatga
     3601 ggtggtagcc ggcacgtggt ggcagccagg ccagcgattt tcagcaccgc aggtttcgat
     3661 tgaggaagaa gccgccagat ccctgggctt agtcgtaggg tcccaactgg aactagacat
     3721 acaaggcgcg actatcgctg cagaggtttc aagtattcgc aaggtcgact ggggtaattt
     3781 ttcgacgaat ttctacatga tcatgtcgcc tggatcgctg gacggcgctc ccttgacgta
     3841 tgtggccacc atccatgtca ctcccgaaca ggaagtgccg ttgcagcagg ccgtcgtagc
     3901 ggcgttcccg aacgtcagcg cgattcacgt gggagatgtc cttcgaaatt ttgccagcgt
     3961 tctggatcgg ctcgccttgg ccattcgggc tgtggcgctg ttctgcgtca tgacgggagg
     4021 gctcgtcctg gcagcggcgc tcgcggcgac gaggtatcgg cggctgtatg aatccgtggt
     4081 tttgaaggcg ttgggtgcga cgcgaagtct gattgcccgg atctttgctc tcgagtatgc
     4141 cttgctgggg gtcgtggggg gaactcttgc agccggatta tcgagcgcct tgtcgtggct
     4201 tgttctgacc tacgtcttcg acctgtcgtg gaggctgcat ccaatggtcc tccttacggg
     4261 gctcttgtct acgatggtcc tcacgttggc ggttggcata ctcagcacat ttcgtattct
     4321 cgggcagagg ccgctggcca tcctacggca cgaataggaa ttcgtcactt tcctgtgatc
     4381 gcttcccagt tcgcttgtgt tcaattcgcc acgatgcgag acagtccccc tgtctgagtc
     4441 ataatgtata actcaccacg cgcgtcctcg ccgaagctgg tgatctgacc tcccgggctg
     4501 agcaatggcc acgtgttctg tgagcttggc cgtccgcctt ggagctgaaa gcttctgaca
     4561 aatcccgcgc aaaagtccgc ataaaaatag ctaccgttca cggccggatt gagggtgcca
     4621 cgatagacgt agccgccggt caccgaacag gcaccggcgt catggctgta atccaggatc
     4681 ggtggcgtga gcccgttgct gttacagccg ctcggcggat tgaagcaggc aaaaccttcc
     4741 atgatattcc aaccgaaatt gacaccccgg cctgcgttgg ggccggctac ggcatcgact
     4801 tcttcacgct ggctctgtcc gacgtcgccg atgtacaaca ctccggtcga gcggtcaaat
     4861 gagaagcgcc agggattgcg gaggccaaaa ctccagacct ccggagcccc tccggtcccg
     4921 ttagcgaagg ggttatcggt gccgcaggca ctgcccgtct ctggatcgag acgaaggatt
     4981 ttgcctaaaa gtgagtttgt attctgcgca ttaccatttg gatccccgat tccaccgccg
     5041 tctcctatgc ccgcgtagag gcagccgtcc ggaccgaatg cgagcatgcc gccgttatga
     5101 ttctggttag tggggtgggc gatggtcttg agaatggctt gtgcgccggc attcgcgatg
     5161 tcgggattcg aactgacgcc gtatcgagca atgacaatgt cgcctgctgt attcgtgtag
     5221 tggatataaa agcgcccgtt cccggcataa ttgggatcaa aggccatgcc caacagtccc
     5281 tgttcgccac cggtgacaac cagtcctgag acatcgagga agggagtggc ccgcggcgtg
     5341 ccatccagag agttaaggat tctgatcagg ccgccttgct cgacaatgaa caagcgagcc
     5401 acatcgccgg ccggggcagt gagaaaaacc ggcgaagaaa gcaccgcagt gacggtctgt
     5461 agtcgcaaac cgttggaagt agcagggggt ggactggcgc cgccacctcc gacgtcatcg
     5521 ctgccgttgc aagcgaccac gagggcgcag agcataacgc cgaagatgcc gatcgaacca
     5581 gctgatgaag gcataagacc agactactat gagacctctg ttggctcaac cgggaaactg
     5641 gtttgagcaa cgtgggtcga atactgattg ttccgcagga attcctgaag ctcgcgccgg
     5701 cttcgctcgc cgaatgagac cccaattcga agctgctcga ggtattgttc gagcgttttc
     5761 ccgcccaagt cgatttgacg ggcctgaaag aactcacgga cgtctcgttc aagctctggc
     5821 ttgcagaaac tcgtgatccc accgcacatc cggcggagcc cttgcttagg aaaccggcgg
     5881 tccagaagat cccaattcgt cttcacgaag cgccaaccct gttcgcgccc gtacacagtg
     5941 ctcatcactg cactgaccat aaagggcgca tcttgaagac gaatctcccc gttaagggtc
     6001 atgttcaatg tccggtcgag tagagcggtc tgctggaaca tcgctaaaga aaacagatac
     6061 ctccgttcct cctgaggcgt ggcggccgtt ttgaagcgtg tccgaaagtc ctcatagcgt
     6121 gccgcatcgc cgatgaaggc gagaatagaa accaatgcag gaaggaggtt cggatcgatt
     6181 gttgcgggat ctttccgcgc agtggcgtag agttcattgg cgcgcgcttg ggttgccggg
     6241 tcattgccca gtcgacccaa ggcgccgatg agttcacctc tgagctggcg cgtcaggtca
     6301 ttttcgcccg gtcgggttgt ccaaccgaca gccgcagcgg cctcgccgac tctggatcgg
     6361 acgaaggatt cgagtccggc tcgatccgcc ggttcgatga ttcgattgag gaaggcaaag
     6421 gaatcgatga ggacggccca gacattcttg tctcgctcca gcgagaagcg gccggtgagg
     6481 tcgagatagt cacacagcgg catcaagccg gcgaccgtcg tcgcccaggc gtcgttgacg
     6541 agattcaatc gctcgatcgg ggccagcttc tccaggccgg cttcaagcaa tcgttttaac
     6601 aagttcgggc cgtaccgcac ccggtagaag ccatgtccgc cttcattgac gaggatgaat
     6661 tgcgtctcag gtggacagct cacgttcaag cgggtgcccg tgagcaacac ccgtcgaacc
     6721 tccccctcag ctccggtctg cacgcgcagc tgcaccggaa cctgccaggt ggccggcggc
     6781 ggatcgggaa gatagctgaa gcgttgttga ctcaacacca gttcgcactg tttcgtgagt
     6841 tcggcggtga ccagcggata cccgggttgg aaaatccacc cattcatgag gtcggggacg
     6901 gcctgattcg atgctttgcc aatggagacc cagaggtcgt ttgtgtcggc attgccatag
     6961 gcatgggtcc ggaggtactc acgcacgccg tcacgaaata cggtcgggcc gatgtgctgc
     7021 tcgagcatgc gaaggacgga ggcgcctttt tcatacgtga gcacgtcgaa catagcttcg
     7081 gcgtctttgg gcgcacgaac gggaaattca atgggacggg tgctgtgcaa cccgtcgacg
     7141 gcaaacgcgg ctccacggga ggcaccaaag gaatcccagc gcttccattc tggcttccag
     7201 gcgtccacgg ccagcatttc cataaaggtc gcgaaagctt cgttcaacca aagtccgttc
     7261 caccaggaca ttgtgaccag atccccgaac cacatatgcg cgttttcgtg tgcgaccacg
     7321 tccgcaactc gttgtagctc accgtgggtg ccggttcgtt gatcgacgag cagcgcagtc
     7381 tcccggaacg taatggcgcc gaggttttcc atggccccgg aggcaaagtc cggaatggcc
     7441 aggaggtcca gtttgtcacc cggataagga atgccgtaat agcgctcgaa aaactgcagg
     7501 gacgctgcag cgatgtcctg tccgaacccc gtgaggtgcg tcttgcccgg tactgtccac
     7561 agctgtagcc gggtcttgcc cacgacagaa ggagcactgg cttccatccg accaacgaca
     7621 aaggccacca gatacgtcga catcttgatc gtgtcggcaa aacgcacgat tttctttccc
     7681 ttttctcgcg cttccgagag gacgaggctg ttcgatatcg cggtgaggct ctcatcaatg
     7741 acgagtgttg tggagaagac ggccttgaaa tcgggttcat cccaacacgg gaaggcgcgt
     7801 cgcgcgtctg ttgcttcgaa ttgcgttgcc gcgatcgtct gttggcttcc gcgttgatct
     7861 gtgtaggtgc tccgataaaa gccgcgaagt ttatcattta gtgtgccgcg aaagctgata
     7921 tgcagctgcc atgttcctgg agccagcggc tcagcgactg tcagttgaca gcgttgcagc
     7981 gattcgtcga gcgtgactga cgcggtctgg cgtcgcccct tcactgcctc gactatggcc
     8041 tcgtcgatgg ccaactccag cgcgttgcag acaatgacgc gcgtctgttg cagcaccgtc
     8101 aatacgatgg tcgcacgtcc taaaaaagta gcgccggcaa gatccggctc gaatgtgagt
     8161 tgatagcgtg ttggacgcac atgctgcggg agccgataag gatcgtcatg accgtaaggg
     8221 acctcgttca tatctttccc tttcgatgca gttggtgcag cgtcaacggt ggccggaggg
     8281 acgcggcgga ggcacgcgaa taacggaatg gccaagagga atcctgtact cctgacaaac
     8341 cgtcgtagcg tttcacgtct tgtgaacgtc ctttcatccc cgcttgacgt gatggatctc
     8401 cgttcgatcg ggagttccta caatcaggac gtcggttcgg ccgacaaaga gaccggcctc
     8461 caccacaccg ggaatccggt gaagcgctgt ttccaattca tcgggccggt cgatccgtgc
     8521 aagatgcaca tccacaatca ggttgcccga ctcggtttgg aagcgtgcgc cctctcgttc
     8581 acgaaggacg acacggcttc gggtgagctc ttcgattttc cgtgccgtgc tgcgccatcc
     8641 aaaggggacg acttcaacag gcagtggaaa gtcgcctccc aacacaggca cgcgcttcgt
     8701 gaaatcaacc atcacgacga acttcatggc ggatgcagca acaattttct cttttaagag
     8761 cgctccgcca ccacctttga tcaagttgaa cgcgggatcc acttggtcgg cgccatcaaa
     8821 agcgacatcg atcttccagc aggtttcggc gtcaatgagg gcgatgccgc actgtcgggc
     8881 gagcatcgcg gtcgcctgtg acgttgagac gccgcgcagg ttcacgccgg ccttcacttt
     8941 ttcgcccaag gccatgatca ggtgcttggc agtcgagccg gtgcccaatc ccagaaccat
     9001 gccgtctttc acgaactcaa cggccttcac ggcggctgca tgtttgaggg tgtcgaggtc
     9061 agacgtgctc atgcttgcgc caaccggacc gccgtcgatg cggcgccgag aaggccggta
     9121 tgttgattca tgacgacgtg tacggggatc gtgcccatca gccgcttgta acgacctttg
     9181 ttggtaaagg ctttcatgaa cgtgccgtct ttgagtttgg ccaagagctt cggagcgatg
     9241 ccgcctccca catagacgcc attgatggcc atggctttga gcgccaaatt cccggcttct
     9301 gcaccataga tagaggcaaa aagatccaag gcttgtttgg caatttcagc ctggcctttc
     9361 aatcccgcgt cggcgatttc ggccgcgggg tcaccggctt tgattttctc agccagccag
     9421 gtcggttcgt ttcgcttcgc atcgcggaga aattcataaa tggcatgcag gccagggccc
     9481 gagagcaccc gctcataact gacatgcaga tactgactgc ggatgtatcg gaggaggtct
     9541 atctcatgat cgctgttcgg agcaaaatcc gcgtgaccgc cttccgacgg tatgggttta
     9601 tagcgggttc cgtcccagaa caggatggat tcacccaggc cggtccccgc cgcaatgaga
     9661 gcaattgcct gcttttgcat tggcggcgtg ccctggttca acgtcaccaa ctcatcggca
     9721 cggagcagca caatgccgtg cgccatcgcc tcgagatcgt tgagcagctg tacggcgggt
     9781 aattcgaacc gcttggccag tgccgcacca tcaaccaccc agggcaagtt agtggttcga
     9841 ctatgatttt gaatgaccgg tcccgccacc ccaaagcaag ccgcgcgaat ttttggcagt
     9901 tcgtcctgcg gggaagataa cggcttgtcc tgcggttccg gctcaaggtg atcgatggga
     9961 gtcggcggtt tgggcggcgc aagaaactcc tcaagcatgt cttcgagcga agcatagtcg
    10021 gcgctatgga atgtttcgag ccgcagaggg tcgactcgat cggtcgtcca atcaaacagc
    10081 gcgagatggg ttttcgtccc cccgatatct ccggcaagga tcatatttgc cccggcgaat
    10141 tgctttcacg atgtccggtc agctgagcgg cggcgggcgg atccaacaac cagatgaggc
    10201 ggcctccatc cggtcggacg agagcggcag gcaacagccg gtcggtttcc tgttggggtt
    10261 ccaaaatggt ttttacgatt ccggccttag ccgcgccagt gacgaggaac agtaccacag
    10321 tcgcttgatt gatcacacct agggtgaggg tgagcctggt ccgagggccg gaaggggcgt
    10381 atcccactgt cacgcagcgc cgtcgttctt gcactgcatc ggtgccgggg aacaaagagg
    10441 cagtgtggcc atcttgtccg atgcccagca atacgagatc caatcggggc caccgttccg
    10501 gcggcgtctt ggtgacagca cgcaatagcc cttcgtagtt gtgagccgca acgtcggcat
    10561 cggcctgttc gcaggccata cgatgaatat gatgtgaagg gatgttcaga gggcgaaaaa
    10621 gggtttcgtc ggcaagatcg aaattgctgt cagggtggtg tacaggaacg caacgttcgt
    10681 cgctgaagaa aaactgcgtg ttgttccaat cgtcttgatc gcaaaagggt gcggaggcca
    10741 atcgtcggta cagttgctcc ggcgtccggc ctccagagag cgcaataaga aacagccccc
    10801 gctctttcac cgcctgccgg tgcaattcct gggtcaggct cgcggcttct gtcgcccatt
    10861 tggatgagtc ttgagcgact cggatctcag cacgtgacat ggctctagtt atttcgtggg
    10921 cgacggtcga tgacttgttg cgaccgcttg acggcggccc tcttcgacac gaggctgcac
    10981 tctatggtgc cggtcccacg aagcgtccaa gaaattgcag agcgttgcga tgcatgattg
    11041 gctaggcgag gcaggcgcat gaggcaagca cgaatccacc atatgggcat cttaggtacc
    11101 gaattgtaaa aaggcaaccg ccggctatga cgacggcggc gttcgtcgaa tgaggaaggc
    11161 catgccctca gtgacggtcc aggccagcga ttgcgcatga tcagatgtga cgctcaaata
    11221 cagccatggc tggggaagag gtcctttccc ggtcgtcaac gtgatggccc aatctttgag
    11281 ccacatccac cattcacgtc ctttaaatcg aagccatcgc cctatgtcgt cggacgccga
    11341 agggtgaacg gtcagcgaga aggtcgggga aaactgcagc gtaaaatcgg ttggcatgtc
    11401 atccagagag atgggtggtt gttcaccgcc ggccgcgggc tcgattttgc gtctggtcac
    11461 cggtggccgc gtctgcagac ggaacgtggc cgaggcgtct gcgagttggc cgcccgacca
    11521 ctgctcgatg gggaagcggt gcaactcgat accgcggccc ttgatgagca cggcacgatc
    11581 gacgagatcc acaatgacgt acgtctgcgg acgagacgcc aacttcacct cttccgcaag
    11641 aacgcgggtg gcttcttgca gcgcagccgg attcgtcacg tcgactgaca ggggatactc
    11701 agcatccgcc gcttgagtag gcgaagcggc catcaacagg aaggaccaaa tgagaatcca
    11761 ggtgacggat gggcggggca ttcagctaga aaatcatgat gggcgtgccg accttggcca
    11821 ggcgatacac actcttcaga tcgtcatcgt tcagtcgaat gcaaccgtgc gtgacattct
    11881 ttcccagaag tctcgtatag agggtgccat gaatgaaaaa gcctttgccg aaccccagtg
    11941 catattcccc cagtacgccc ggctcgatac ggtccgtgta actcttcggg atggccaatc
    12001 cttcttcaac gaaggcccag tccggcttta cccacacagg attgatgagc ttcgattgaa
    12061 cggtgaattc ccctcggggg gtatcaaaaa cccattgacc tttggcttct ccgggcttgt
    12121 ccagaatcgt gccgctcccc gttgaagcga gcgcatccaa catcacgtga tcctggcgct
    12181 tgatataaag atggtttctc gcagtatcga cgacgatata cggctggctg ggcatcagct
    12241 ggttgagctg tttggacagg gatcgatagc ggttttgcaa gacgtgtaat gttgcgggat
    12301 cgggtacgac ggccggaatg gtcgaggcat cggtgacagg gagactgctg ctctgtgcca
    12361 gcgtcaacag gactaggccg atgagtccca agcagaggga ggccacaatg cgtgtcatgt
    12421 tccgtcaaat ttcgtttcga tgcacagaaa gactcgcgag ggcctttgcg actgaattct
    12481 gttcgagaag cgctccgact atggtcaccg gcgtaccggc gtccacacga tcgaagaggg
    12541 cggccatctg ggcgttctcg agcatgacgc accccagcgt ttgagccatc aactcgttct
    12601 ccacgccatg gatctcgatt tgccctccaa taccgcgcaa cgtgggaata ttccccatct
    12661 tgcgatcgga gagaaagcgc cgccgatcgt cctggttggg atagtccagc agcaaggcgc
    12721 gatagaactg ggtctgccct cgtcctcgtt tgctcaacac gcgataccgg ccttcaggtg
    12781 tcgcgccatc gccttgatac cgcttttcct tgatgccgtt aaagcccagt cgaacggggt
    12841 aggataccac tttttgtccg ttgcgataga gggttaagga tcgctccgct ttgctgacga
    12901 cgatggcggt cctatggtgg ttccgcgacc attcgatcgt ctcgctggcc atgcgctgcc
    12961 agcgggcaac gcgatctggg ctggcgtacc gccctaattc tcgttggagc aaggcggact
    13021 gagccgcgag gtagtgggaa gcacgacgag aggcgctcaa tgactgttcg tatcgtcgtt
    13081 gttcgtaaaa tgtacgggcc tgcttcaaca gtaagtcggt ttcgaccggt ttctcgccga
    13141 gaatgagtcg gctgtcaatc gctcccacgt gagtggccag cagatgggcc tgttcttcca
    13201 cttcagcaag ctggcgtgcg gccgagcgtc gtagcatttc ctgctgttcc atcaggagcg
    13261 cgacggttct cgtcccttcc gcatgcaggc cgcggagcgc ttcttccaag tcattcggct
    13321 cccaaggcca gcgaacggta tcttcttcgg cctcgacacg gcttcgcaat gtgacccatt
    13381 gatacgcaaa ggcgttgtac gcactcggcg ccagctcagc cgcccggagc tgcgccaaat
    13441 ctttatcaac tgattcaatg gcctgcacga cgtcgggggg cacgctttct acgcatgcgg
    13501 ggagcgaccc gcacagcgca aagaggcttg aatagcacac gatccgcact gcatatcgaa
    13561 gtgacatctc acgcataggc actacttctt cttggtgacc ggtttccctt tgccgatctt
    13621 ggccaacgcc gtctgaatct catgtgagac ggcttcggct ttttcctgaa tcgcctttgc
    13681 tttggtttgt gctccgagat aatccgactt gtcgaacatc aactgtacgt cgttgaggga
    13741 agccttgagc gcttgtgcat ccgttttgat cgattcaagt gccgcgcggt ctttgccggc
    13801 gggagccctg gagacgagct cgagcgtcga ctggactgcg gcctcggctc catgttgagc
    13861 ttgggtggcc gcaaacttcg cttcttccat tttcttggtg gcatctgcct tgacgcgttc
    13921 ggcctcggct ttcgttgttg cggcgagctg ttctgccttt ccatagtctc gaagcaaggc
    13981 aaacttcgcg tcttgatctg caacttcctg cttcatggcc gccagcaatc cttcgacctt
    14041 ggcatggtcg ttggcagcat acgtcgcggc tccgctttgc tgagcgtctt tgaccgcctg
    14101 ttccgcggcg ttgatctggt cggctggcgg ctgtgcgcaa ccggcggcca agaaccatac
    14161 gctcaggact gcgaccaaga aatggcgcat tttcatatcg gcgttcctcc tgattaagga
    14221 cgcaagtagc gtccacactt ggtgtgccca cacccgtaca ctgcctggaa tcccaataga
    14281 tttcgcaaag gtagtgccag agcgacgagg agcggacact cgtcctcgcc ttgattcagc
    14341 aacacgttag agcgtgcttg ctcattcgta ggaataaact gtgtggcagt ctagttcatt
    14401 ttatgcaaaa ggtccacaaa actgcctcaa tctaaatggg ttatcaaaga cttagccggc
    14461 atgcctgcaa gcaagatcct cctcaatcac cggcaacaag tcgagaggtc gcgaggtttg
    14521 acagggtgga ggctcgtccc tataatgcga ccttcattcc gtatctgtcg gaaaggcgat
    14581 ttgtatgtcg tttacgtttc aacagccatc gaacctcaaa aagaagcgcg aacacggatt
    14641 ccgaaaacgc atgagcacaa agaacggccg gaaggtcttg gcccgccgcc gagccaaggg
    14701 tcgcgcgcgt ttgacggtct agcactgctt ttcgacgccg cacggagttc atcccccttg
    14761 tcttcctcaa gcagggacgt ctttctaaga acctgacggt gagcggtccg gccgagctcg
    14821 ggacgcagag tcgagcggag gggtccggac aagcccctac gaatcatcct gatcatgtca
    14881 cggagggcag gagatcgaat cctttttctt cgcgcgagcc gcgacattga atttgtgaaa
    14941 cagcatggcc ggcggctgtc cacagcctgc ttcaacctgc tggtgtgcaa gaccgaaggc
    15001 ggcacgagcc gcgtcggcat cattgtgggg cgacggttcg gaattgccgt tcgtcgcaat
    15061 cgagtcaaac gtctgttccg ggagctgacg agacagattc gaaacgagct gacttccaat
    15121 catgccttgc tcgtatttcc aaagcgagac gccctcacac ttccgttcca agaactgaaa
    15181 ggcctgtggc gggctacgct gcggcgtcaa ggtttgctca gtggccgtga ggtatagcat
    15241 gaaaacgatg tgtgtggctc tgatcaatgg ataccgattc tggatctctc cgtggctagg
    15301 gccagcctgc cgttttgacc ccacttgttc cgactatgcc ggccaggcga tcgagcgcta
    15361 tggggttatc cgaggagcca gccttgctgc ctggcgcctg ctcaagtgtc atccgtttca
    15421 cccaggcggg cacgatccgc tgatgcccga agtgaccaat cgcgggacgg cggagtgaga
    15481 tggagaaacg cgtcgtcatt tttcttgtgc tttcgttggc agtcatcctg ggctatgacc
    15541 tcctcctgaa acaattgggg ctgctcccga cgactccgac cgtgcaagag ccagggggcc
    15601 aggaggctac tgctcctcgc ggtcaaaccg agaggccttc cgctgaaacg acgccgagca
    15661 ctccgcgttc cctctcaccc gcttcggaca ccgcctcagc cggtgattcc cgcacctcta
    15721 ctcctgccac ggaacgcacc gtcaccgttg agaccgattt catacgagtg gccttcaatg
    15781 ctcgtggcgg ggtgatttcc agttgggaac tcaagcggca ttacacgtcg gctaccgacg
    15841 gaaacccggt ccaactcgtg taccaggggg ccaaattcaa ggaaccgctc tcgatcacaa
    15901 cgtcggatgc cgcggcagac aaggctttac gcgaaggtct gtatgcgatt gaagccgatt
    15961 tcaccacgct cgatgacgcc catccgaccg gccatgtaac cctgcggttc cacgatcctg
    16021 cattgggcct caacgtacaa aaacgcctga cgtttcgtca ggaaaattat ctggtcgatg
    16081 tcagtttgac tgtcgatggc ttgagcggtg ggtacgacat tgtcctaggc acaaacttcg
    16141 ggattgtcga atggggagac ggtttcatcg gtttgatcgg ttcggcttca ctggtagatg
    16201 gaaaagtcga aaaagaaacc cctgatctcg aattcgaacg caagggatcg gtgcaatggg
    16261 ttgccctcca agacaagtat ttcctttctg tcttgatgcc tcagggtgcc agcgcggcat
    16321 tggccaagaa agaaggcgac aaactcgtgt ctgccggcgt gcgaatgccg gcttccggcg
    16381 cacgtaccac cgacctgcaa ttgtacgccg gccccaagga atatgacacg ctgcgatcat
    16441 taaacgtggg cttggaagaa accatcgact tcgggtggtt catttttggc agttggagcg
    16501 tcgtcaaggc agtggcgaag cctatcttct acgtgctgcg tttcatcaac gaatatacgc
    16561 acaactatgg tgtcacgatc attctgctga ccatgggcat caagatgctg ttcgtgcccc
    16621 tgcagtacaa gagctataag tcgatgaagc agatgcaact gatccagcca aaggtgctgg
    16681 cgctgcagga aaaacataag gacgatcggg acaaattgaa taaggagctc atcaagctgt
    16741 atcgtgatca caaggtcaat cctgtggggg gctgtctgcc gatggtgctg cagatgcccg
    16801 tcttcgtggc gctcttcaac atcctctata tgaccatcga tctccgacag gccccttttg
    16861 tcgggtggat tactgacctg tccgtgcagg acccctatta cgtgctgcct atcatcatgg
    16921 gcgccacgat ggtgattcag cagaaaatta caccgacgac gatggatccg actcaggcaa
    16981 aaatcatgtt ggtcctcccc gtgtttatga cttttctatt cgtcaatttt ccggcaggcc
    17041 tggtgttgta ctggctaacc aacaatgtgc tgaccatcgg acagcaggtg ttgaccgacc
    17101 gcctctttgg cagcaggccg ttcgtcaccg atccagcaaa atgagcgaga ggatccatgc
    17161 agcgcggagg acccgacgat acaatctgcg cgatcgccac tccggccggt gaaggcggga
    17221 tcgggattgt gcggctcagc ggcccacagg cgctgcggat tgccgacaag atcgtgcagt
    17281 tgcgatccgg tcagccgttg gcatcggtcg cgtcccacac cctctgcctt gccgacctcc
    17341 gctttcccga catgaatcag acggtcgtct cacctgaccc gtccctgccc aagccacgag
    17401 agtgtttcga tgaggcactg gtcgtgtaca tgaaggcccc tcgatccttc accggtgagg
    17461 atatcgtcga gattcagtct catggaggct cactcatcct gtctctgacc tgccgcgcct
    17521 gcctcgagcg cggcgcgcgt ctggcggagc ccggagagtt taccaagcga gcctttctca
    17581 acggccggct cgatttgtcg caggctgaag cggtgctcga tacgattcgg gcgaaatccg
    17641 cagccagtct caccgcggcg cagcgacagt tgcggggcga tttggccagg gaggtggaca
    17701 cggcaagaga agcgttagtg cacctgctgg ctcagctcga agccggtatt gattttagtg
    17761 atgaagacgt tgacatcatt caaaaacagg atttgctcga gaccattgat cgggcggcca
    17821 tgctggtaga acgactgaga gccaccgcac ggacaggccg actccttcgc gacggggcac
    17881 gcgtcgtgat cgccggccgt cccaacgtgg ggaagtccag cctgctgaat cgcttgctga
    17941 aggaagaacg ggcgatcgtc acagcggttc ccggcacgac gagagacctg attgaggaag
    18001 ccatcgacct cggcggggtc ttggttcatt tggtcgatac ggcagggata cgtgacacgg
    18061 aagatccggt ggagcatgaa gggatcaagc gctcatggtc ggctcaggag caggccgatt
    18121 tgcgcgtcat cgtgcttgat ggcagcctgc cattgaccgc cgacgatcgc cgcttgatgg
    18181 atcaggccgc tgagatgagg cacgtgatcg tgatcaacaa ggccgacctc ccggcccagg
    18241 tcagtccgac gtctgtaaat ccgggcagcg tgtgcatcgc ggtctcggca aaaacgggag
    18301 acggggtcga ggacttgcga ctggcgattc gagcgcaatt gactggaagc gggcccgaga
    18361 ccgccgatgg c
//
LOCUS       DASTQY010000388         3840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_545108, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000388 DASTQY010000000
VERSION     DASTQY010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545108"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFR82_12645"
     CDS             <1..228
                     /locus_tag="VFR82_12645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEU4502492.1"
                     /translation="LKKIGLLRIDGDWYESTRICLETLFPMVSSGGIVVIDDYGKWAG
                     CRKAVDEFMETLPRRLLINHIDAAGRYIIVP"
     gene            293..1000
                     /locus_tag="VFR82_12650"
     CDS             293..1000
                     /locus_tag="VFR82_12650"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4502493.1"
                     /translation="MSNNPQVSPGRLEYSVRRAFVDEFYMRQASSLKAGSRVLDLGGK
                     RSPKRGQFRIEHYDVRVRYANLSAANRPDVQTDAATLPFKNETFDAVICAEMLEHVAD
                     PVSVIREAYRVLRGRGVLLITVPFLFPIHADPYDYCRYTDHYWRENLRKVGFVDLAVE
                     PQGYFWSVAIEMARAWCYERVKQGRLQSRLLQRLIGALVARGKRIALSREQRVEASDH
                     AFITRYTTGYGIRVVRP"
     gene            997..3021
                     /locus_tag="VFR82_12655"
     CDS             997..3021
                     /locus_tag="VFR82_12655"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4502494.1"
                     /translation="MKRPKVFLTGGDDIGWALDEDLRLTRKALEGFVEFTDLETCEVV
                     HSVWWEPLLSIAPATLSAKRIVCHMAGEPFRYLSLPRHRHVVGAVDCWISQTTQAARQ
                     CGAIGLTHTCIPYATDAEAFHRLPPDNETLDEFRTRWKIPRNRYLIGSIHRDSAGFDL
                     SIPKKVKGPDILVEIALALHRRGCAVHLVLAGPRRHWVRRHLEDWGVPYTFIGEITAD
                     DDYARNVLPRETVNLLYNLLDLYLVTSRSEGGPRSLMEAAAAQCKVLSTRVGLAEDLL
                     DPVCIYTSPIDAVVLIERDITDNRLADTVRGQYDRVMARHRPETIAPLFQGLYRSLAT
                     ELAERKPTQAAWPQPVSPAPPEQTFVRRLRSLVSRKRFRVGLWHRFFAPPYGGGNQFM
                     LALRNALRRRGITVVENELRDDVDVYLLNSIHFDVDRFLEFAQRHRLRAVHRIDGPIH
                     LIRGFDREKDELCFELNKRFANATVLQSTWTYQRIAEMSYQPVKPVIIRNSVDPVVFH
                     SRRRIPFDPGRKIRLIATSWSNNARKGGPIYKWLEDQLDWDRFEFTFVGNASVPFTRI
                     RTVPAVPSEQLAEILREHDIYITASQNDPCSNAVIEALACGLPVLYRNDGGHPELVGY
                     GGLPFAEQEDIVPQLETMAANYEMFQRLIAVPTMDEVAESYLTLLREVAA"
     gene            3041..>3840
                     /locus_tag="VFR82_12660"
     CDS             3041..>3840
                     /locus_tag="VFR82_12660"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4502495.1"
                     /translation="MEVTNHIQPAGHVRGFREAITAMARVNDDAFFGWFDHTEDTESA
                     FVRGSWDFMLHIARPLAPWLARPEQRVALEIGHGGGRILAAASRCFQTVIGVDIHDEN
                     EKVQAALRARGVTNARLMKTEGALLPVESELVDCVYSFIVLQHVETYSVFTRYFEETF
                     RVLKPGGIAVLYFGRRYRWSFNRSSRLLYAADRLMEPVLLRHGYEELPAPVNSTNLRV
                     SLRHAKSLSRSLGFSVMRTLVSRRRVPDGVTLYGGQHGLVLRKPPCAR"
BASE COUNT          771 a         1084 c         1176 g          809 t
ORIGIN      
        1 ctcaaaaaga tcggactgct tcggattgat ggcgattggt acgaatcgac aagaatttgc
       61 ctggaaaccc tgtttcccat ggtcagctcc ggcgggattg tcgtgatcga tgattacggg
      121 aagtgggccg gatgccgaaa agccgtagac gagttcatgg agacattgcc ccgccgcctg
      181 ctcataaatc atatcgatgc ggccggtcgt tacatcatcg ttccctgacc cgacggacga
      241 ggggcatagt gccgcgtcgc gcggcaggac gagaacgagg gagtgaaggc cagtgtcaaa
      301 caatccgcag gtctcgccgg ggcgattaga gtattcggtc agacgcgcgt tcgtcgatga
      361 gttctacatg cgacaggcgt cttcgttgaa ggccggcagc cgggtgttgg atctcggagg
      421 gaaacgctcg ccaaaacgag gccaatttcg catcgagcat tatgacgtgc gcgttcggta
      481 tgccaacctc tcggcggcaa atcggcctga tgtgcaaacg gatgccgcca ccttgccgtt
      541 caagaatgag acctttgacg cggtcatttg tgccgagatg ttggaacatg tcgcagaccc
      601 tgtgagcgtg attcgagaag cctatcgggt gcttcgaggg cgtggcgtac tactcatcac
      661 ggttccgttt ttgtttccca tacacgcaga cccctatgac tactgccgct acacggatca
      721 ttactggcgg gaaaacctgc ggaaggtggg ttttgtcgac ctggcggtgg agccacaagg
      781 ttacttctgg agcgttgcga tcgagatggc gcgtgcgtgg tgttatgagc gggtgaagca
      841 aggccgcctg caatcgcgac tgcttcaacg gctgatcggc gccctggtgg caagaggcaa
      901 gaggatcgcc ttgagccgcg agcagcgggt cgaggcctcc gaccacgcct tcatcactcg
      961 ctacacgacc ggatacggca ttcgcgtggt gcgcccatga aaaggccgaa ggtgttcctg
     1021 accggaggcg acgacatcgg atgggcctta gatgaagatc tccggcttac gcggaaggcc
     1081 ctcgaagggt tcgtcgagtt cacggacttg gagacctgtg aagtggtgca ttcggtctgg
     1141 tgggagccgc tgttatccat tgcgcccgcc accctctcgg caaaacgaat tgtctgtcat
     1201 atggccggcg aacccttccg ctatttgtct cttcctcgcc accggcatgt ggtcggcgca
     1261 gttgattgtt ggatcagcca gaccacgcag gcggcgaggc agtgtggggc cattggtctc
     1321 acacacacct gtatcccgta tgcgacagac gcagaggcgt tccaccggct tcctcccgac
     1381 aacgagacac tcgacgaatt tcgcaccagg tggaagattc ccaggaaccg ttacttaatc
     1441 ggcagcatcc atcgcgattc ggccggcttc gatctcagca tccccaagaa agtaaaaggt
     1501 ccggacattc tcgtggaaat cgctctggcg ctgcaccgcc ggggctgcgc cgtgcatctg
     1561 gtgctggcgg ggccgcgccg tcattgggta cgacggcatc ttgaagactg gggagtgccc
     1621 tacacgttta tcggcgagat cacggcggat gacgactatg cacgtaatgt tcttccccgt
     1681 gaaactgtga acctgcttta caaccttctc gatctctatc tcgtcacgag tcgatcggaa
     1741 ggcggtcctc gatcgctcat ggaagcggcc gctgctcaat gcaaggttct cagcacgcgg
     1801 gtgggtcttg cggaggacct gttggatccc gtctgcatct acacgtcgcc aatcgatgcg
     1861 gtggtattga tcgaacggga catcacagac aatcgcctcg ccgacaccgt tcgcggacaa
     1921 tacgaccgcg taatggcccg ccaccggccg gagacgatcg cgccgttatt ccagggcctc
     1981 taccggagtt tggcgacgga actggcagag aggaaaccga cgcaagccgc ctggccgcaa
     2041 cctgtgagtc cggctccgcc cgagcagacc ttcgtccgtc gtctccggtc tctggtgagc
     2101 cgcaagcgat ttcgggtcgg cctttggcac cgtttcttcg cgcctcccta tggcggaggc
     2161 aaccaattca tgctggcgct gcgcaacgcg ctgcggcggc gcggcatcac ggtcgtggaa
     2221 aacgaactca gagatgacgt ggatgtgtac ctgctcaact cgattcattt cgatgttgac
     2281 cggtttctgg agttcgctca gcgccaccgg ctccgcgccg tacaccgcat tgatgggcct
     2341 attcacctga ttcgaggatt cgatcgagaa aaggacgagc tgtgctttga gttgaacaaa
     2401 cggttcgcga acgcaaccgt gctgcaatcg acctggacct atcagcgtat cgcggagatg
     2461 agttatcagc cggtcaagcc ggtgatcatc cgcaattcgg tcgatccggt ggtctttcac
     2521 tctcgtcggc gcatcccatt cgaccctggc aggaaaatcc ggttgattgc cacgagctgg
     2581 tcgaataatg cgcgcaaggg agggccgatc tacaaatggc tggaggacca gctcgattgg
     2641 gaccggttcg agttcacgtt tgtcggcaat gcatcggtgc ctttcacccg catccgtacg
     2701 gttccggccg tgccctcgga gcaactggca gagatcttac gcgagcacga catctatatt
     2761 accgcgagtc agaacgatcc ctgctccaat gccgtcatcg aggcgttggc ctgcggcctg
     2821 ccggtgctgt atcgcaacga tgggggtcac ccagagttgg tgggttacgg gggattaccc
     2881 ttcgccgagc aagaggacat cgttccccag ctggaaacga tggcagcgaa ttacgagatg
     2941 tttcagagat tgattgcggt gccgaccatg gatgaggtcg ccgaaagcta tctcacgctc
     3001 ttgagagaag tggcagcgta accgtgtctt cggagtgttc gtggaagtga cgaaccacat
     3061 acaaccagca ggccatgtga ggggcttccg cgaagccatt accgcaatgg cgcgggtgaa
     3121 cgatgacgca tttttcggct ggttcgatca cacggaagat acagagtctg cattcgtaag
     3181 aggaagttgg gatttcatgc tccatatcgc ccgaccgctg gcgccgtggc tggcgcgtcc
     3241 tgagcaacgg gtagccttgg agatcggcca tgggggcgga aggatcctcg ccgcagccag
     3301 ccggtgtttt cagacggtta ttggggtaga catccacgac gagaacgaga aggtacaagc
     3361 cgcccttcgg gcccggggcg tgaccaacgc gcggctgatg aagaccgaag gggcgctcct
     3421 acctgtcgaa tccgaactgg tcgactgtgt gtattcgttc attgtgcttc aacatgtgga
     3481 aacatattcg gtcttcacac ggtacttcga ggaaacgttt cgcgtattga agcccggcgg
     3541 aatagccgtg ttgtatttcg gacggcgcta ccgctggtcg ttcaatcgct cgtcccggct
     3601 attatacgcc gccgaccgcc tgatggagcc ggtgctgctt cgtcacggct atgaagagct
     3661 ccccgctccc gtgaacagca cgaacctcag agtgtcgctt cgccacgcga agagcctttc
     3721 ccggagcttg ggtttcagcg tgatgcgtac gctcgtatcc cgtcggagag ttccggacgg
     3781 tgtcaccctc tacgggggac agcacggcct tgtgctgagg aagccgccgt gcgcgcgaaa
//
LOCUS       DASTQY010000389        16280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1494167, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000389 DASTQY010000000
VERSION     DASTQY010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 16280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16280
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494167"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VFR82_12665"
     CDS             complement(<1..142)
                     /locus_tag="VFR82_12665"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU4502496.1"
                     /translation="MSKDTRIERRCAWAGSQPHMRRYHDKEWGKPVHDDRRLFEMLLL
                     EGA"
     gene            491..2032
                     /locus_tag="VFR82_12670"
     CDS             491..2032
                     /locus_tag="VFR82_12670"
                     /inference="COORDINATES: protein motif:HMM:NF025714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HEU4502497.1"
                     /translation="MKYHMISACLAMVALTACSHTIPETYTKPQAPPQLGKTPPVIQP
                     VNAAQKQAQAPESLLPVTMTVDLTPVQKTIQAALPERFTEEGHPLAKDYRWRFIREGE
                     PQVQIQDGLVTFSATYRGEIESAAARACRLDPLYPVLEGTGRLELHEQDQGLLVTMSQ
                     PQTSMSLKPESDTKCNMFNLPVKDQLAELFKQESVKQHITQSLEQAGYVIPLNLIWDR
                     LQEPLPVSSGNTKLCLYGKAKDFAIGSLKGPVQNTTITGVARQTPVALFQTPCQKPTN
                     APLKVHMDTAATAMQEGQPYRILLSVPVPYAVLNQQLQERLYHQGVKLPTTFDNDLTI
                     ERVTASDVSGRTLFAVETSGDTKGTLYYWGTPQLEQEGNMITLPDLQIANETKIALEE
                     IKSGYWQMVDDQLKPRLRQASTLDLSQRIGTMRSALSGQHKSGSLAMDLLVPKQEAGQ
                     VVSTKDALVADVFLEGTASAAGRLPVKQASRSDIDQSHPVGTDRTPAAEAAPKAARNP
                     DEDRR"
     gene            complement(2285..3256)
                     /locus_tag="VFR82_12675"
     CDS             complement(2285..3256)
                     /locus_tag="VFR82_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU4502498.1"
                     /translation="MQYVHLGRSGVKVSRLCLGTMNFGPETTEADSAAIMDRALELGI
                     NFFDTANVYGWKVGEGWTEQIIGRWFAQGGGRREKVVLATKLYGRMGDWPNQARLSAL
                     HIKRACEASLRRLQTDCIDLYQMHHVDRDTPWEEIWQAMELLVREGKILYVGTSNFAG
                     WQLAQAQELARSRHFLGIVSEQSLYNLNERTIELEVLPACEAYGIGMIPWSPLARGLL
                     AGALTPATVGRRADKDVQQEVEKYRPRLEAYEKLCQELSERPADVALAWLLHQPAVTS
                     PIIGPRTLEQLASTMRVMNLTLATETLKRLDAIFPGPGGPAPEAYAW"
     gene            complement(3283..4122)
                     /locus_tag="VFR82_12680"
     CDS             complement(3283..4122)
                     /locus_tag="VFR82_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU4502499.1"
                     /translation="MTLSAYNGVPMPAFMYGTAWKKEATTQLVIEAITAGFSAIDTAN
                     QLIHYDEARVGEALLSVAQGGHTRDRLFLQTKFTPVNGQDHRTPYDAGAPITTQVRQS
                     FESSLTHLRTDYLDSYVLHGPYSRGGLGPEDWEVWSAIESLYASGKTKLIGVSNVTAS
                     QLALLCERATHRPMVVQNRCYAAFGWDKAVRDLCRTQQIVYQGFSLLTANREVFADPG
                     IRAMAEKYGTGVAQIVFRFAMQVGMLPLTGTTNREHMTADLQCDRFTLTPDELQRIET
                     IGL"
     gene            4244..4567
                     /locus_tag="VFR82_12685"
     CDS             4244..4567
                     /locus_tag="VFR82_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502500.1"
                     /translation="MEPESQIVELRFSYRYTKQHTWVVQAVNGFLSAYFMEQPGFRVQ
                     RHFDELESGMHVWLCEVPATMKMTQLLKRLRADIPPFRQTETRSSTDSCAQYVIDSIE
                     PDSSA"
     gene            4648..5736
                     /locus_tag="VFR82_12690"
     CDS             4648..5736
                     /locus_tag="VFR82_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEU4502501.1"
                     /translation="MHRVLVLGAGKIGSLVACLLSQQGAYEVHLGDVTLDAPKHLVED
                     LGLSRITPAVLDVRHPDTVETYLSAHPVDAIISSLPYFCNPTVAALALKHGCHYFDLT
                     EDVEVTNHIRALSAGAARAFMPQCGLAPGFISIVTHELMTHFETLDHVKMRVGALPVH
                     PSNALKYSLTWSTDGLINEYGNLCYGIEDGRKIPLQPLEGYETIELDGLLYEAFNTSG
                     GLGTLADSSVGSVKTMNYKTLRYPGHCEKIQLLMKDLKLNEDRKTLKRVLEHAIPQTL
                     QDVVLIYASVTGTKHGEFFEENYVKKIYPQCIKGKLWSAIQVTTASSVCCVMDIVLSN
                     GAAYQGFVTQESIGLQDFLANDFGACFR"
     gene            5733..7259
                     /locus_tag="VFR82_12695"
     CDS             5733..7259
                     /locus_tag="VFR82_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU4502502.1"
                     /translation="MSTVHAALKDLGIQAVSSGGSTGKHWWSDHATGSLIQSVNPATG
                     EAIAAVRSCSAADYEHIATQARDVFATWRMVPAPKRGELVRLIGQALRSKKDQLGTLV
                     SLEVGKIKAEGDGEVQEMIDMADFAVGQSRMLYGATMQSERPSHRMSEQWHPLGPVGV
                     ITAFNFPVAVWAWNAFLAAIAGDPVIWKPSPKAPLCAIATQQICNQVMEQQGYSGIFS
                     LFITDQAPLAETMVRDPRLPLISFTGSVPVGRHVASIVGQRLGRTLLELSGNNAVIVD
                     HSADLDLAVRAIVFGAVGTAGQRCTTTRRLLVHESCADALLDRLVKAYKQVRIGNPLD
                     SDVLLGPLIDHVAVEAYRSAIDEIKKEGGEILYGGRVLTRPGYFVEPTIVRAQPHWPL
                     LQRETFAPILYAMTFRTLDEAIAIQNGVPQGLSSAMFTNNLLHSERFLSGSGSDCGIA
                     NINLGTSGAEIGGAFGGEKETGGGREAGSDAWKAYMRRQTNTVNWGTDLPLAQGIKFG
                     "
     gene            7287..7883
                     /locus_tag="VFR82_12700"
     CDS             7287..7883
                     /locus_tag="VFR82_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4502503.1"
                     /translation="METNRELDGLLERMVVEYVARNRAAKILKGLLDEAGIGFYPVVD
                     HVTLRTMDIDRRAEPFLALGYAYDETLNYDDWYAKVYRKAGYPALFVDQAYADARGKT
                     SIIPGWVAAFGDQIFHHIAVRVEDIDKAVNRLKTNGVVFAGQIVGPAGGQLRQIFSAP
                     EVVDGQPFTVLELAERHRGYLGFLPPQADSLMKSTAPQ"
     gene            complement(7887..9032)
                     /locus_tag="VFR82_12705"
     CDS             complement(7887..9032)
                     /locus_tag="VFR82_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502504.1"
                     /translation="MGIRILVADDDPDIVMSLSERLRWLGHDVVTAADGQAALAAVES
                     HALDLAFFDVSMPRLNGIEALKIVRKRWPDLPVVVVTAYGTIRLAVEAMKEGAVDFIT
                     KPFEQGQIDQTVTTLVARMGQRVEITRLMGEITHDVKNLLMPLVAGTDLLAEEIDDVF
                     KHLPTLAPAQAEQSHLACDEVITMFRNTSRRIQDRMKAIADYAALTRVSQKFESCLIG
                     KIADSVVKSLRAPAERKQVVLKTEGLHTLPPIVGDEARLYSLLYNLVNNAIPEVPPKG
                     SVTIKGAASAGDDCVTLWISDTGVGMPADMRDNLFTYRLVSTKAGGTGLGTKIIKEVV
                     DMHGGQITVESEPGQGTTFEIRLPIEGPTFLQNSDAAAQAGNSREAP"
     gene            complement(9179..10798)
                     /locus_tag="VFR82_12710"
     CDS             complement(9179..10798)
                     /locus_tag="VFR82_12710"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU4502505.1"
                     /translation="MLRAVMRTDTTERNELAVATAKRRTFAIISHPDAGKTTLTEKLL
                     LYSGLIRTAGMVRGRKGGKTAASDWMGMEQERGISITASAMQFPYKEAIINLLDTPGH
                     QDFSEDTYRTLTAADSAIMVIDAAKGVEAQTRKLFAVCRLRRIPVLTLVNKMDLPGRP
                     PLDLMTEVEQALTIHASAVNWPIGSGSDFAGIVTRADNRVALFSKTAHGGATKVDVQT
                     MGLEDLARSGRVSPDVMAQVQHDLELLTIAGNPLSHEQFLDGEVTPVFFASALTNFGV
                     ESFLDAFVQLAPAPGARLADTDDGGERSIDPIDQPFSAYVFKLQANMNPKHRDSTAFL
                     RVCSGRFERDMVVKHHRLGRDVRLSRPHSLVAQERSTVEEAFPGDIIGIINPGVFAIG
                     DTISVTGGVRFKPLPQFQPEIFARIRPTDVGKRKSFDKGMEQMAQEGTVQIMRSINDL
                     ESLVAAVGRLQFDVLQYRLRHEYRVETVLDTLPFTCSAWLEGDPATFKAPSASMIVKD
                     QRGRVVVLFGDPLMKTIARDRNPDHVLKDMG"
     gene            11581..13125
                     /gene="hpnR"
                     /locus_tag="VFR82_12715"
     CDS             11581..13125
                     /gene="hpnR"
                     /locus_tag="VFR82_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008169 - C-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid C-3 methylase HpnR"
                     /protein_id="HEU4502506.1"
                     /translation="MNVLLVHPGPLMYTKIYLRLEPLGLELVASAARALGHRVTMLDL
                     QVESSNDYVRLLREHCPDAVGFSCNYLANVPEVVNLAKLAKRSLPRCAVFVGGHSASF
                     VADALLVHGEGAIDCVLKGEGETAIGPVLAALADRDNPPGVIPGGITTAGEGPPVRML
                     EHLDAHRPARDLLRHRRKYFIGVLDPCASIEFTRGCPWDCSFCSAWTFYGRSYRVRTP
                     EAIVEDLGTVREPGVFIVDDVAFIQAKHGLAIGEAIARRSIKKRYYLETRGDVLLRNK
                     EVFRMWKTLGLAYMFIGIEAIDEEGLAQHRKRITLSKNFEALEFARSLGITVAINLIA
                     DPSWDRRRFEIVRQWCLEIPEIVNISVNTPYPGTETWHTEARHVTTRDYRLYDIQHAV
                     LPTHLPLPEFYHELVQTQQILNRKHLGWNALKSTARIAAGHLLRGQTNFVKMLWKFNS
                     VYDPRLQLADHARPVLYEMPLPPKRTSGMQTDLLYIHRARGRAGRVLDDATEQFVETT
                     RMGTTA"
     gene            complement(13163..13558)
                     /locus_tag="VFR82_12720"
     CDS             complement(13163..13558)
                     /locus_tag="VFR82_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502507.1"
                     /translation="MFGPITLPFGAKMLFNTVTLKPGVTFEDIELAVGELCSVVKDTY
                     GGDKGGFLAGQVFKFSGFVSEEGSLSSSKTAEDHYVIVTYWRSFEDHERSHANELFTK
                     KFSALAAMCVETKELGYDMLWQGQAGQRS"
     gene            complement(13587..15197)
                     /locus_tag="VFR82_12725"
     CDS             complement(13587..15197)
                     /locus_tag="VFR82_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEU4502508.1"
                     /translation="MEGLHQLEIVILLLGVVLALTTIAQKIVVVPYPILLVIGGLILS
                     LIPGVPTVTLNPDLVFLVFLPPILWAAAYFTSWREFRRNLRPISLLAIGLVVVTSGAV
                     AIVARWLLPGMGWAEAIALGAIVSPPDAVSATAIGKRLRIPRRVVTILEGESLVNDAT
                     ALVVYRMAVGVAIGGSFVPGEGLVTFVFAASVGVAIGIAVSIAARWALCATHDSFTEI
                     AITLLAPYVAWVLGEQVHASAVLACVAGGLHLRQHFSAAVSPITRIQGRAVWDVLVFV
                     LNGFIFILIGLELRALSQAIPPGGLAPVLLTGIVVSLTAIVVRLGWVPLAALIPRWLS
                     ASLRARDPLPAWPNIFLVAWTGMRGIVTLAAALALPVATAAGTPLPFRAEIILISFTV
                     ILVTLVLQGLSLTPLIRALHLEEDRGFEQEEMLAREHAATMALTRLEALADESWSIGE
                     QVERLRDHYGRRMQRFGGNGHVDPECSLESAEAFRRLRHETLTAERMALIGMRNDGTI
                     SDEVLHRLEHELDIEAVRLGIGERRVHA"
     gene            complement(15356..15661)
                     /locus_tag="VFR82_12730"
     CDS             complement(15356..15661)
                     /locus_tag="VFR82_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502509.1"
                     /translation="MALALLLWLLCAGTVDSAFAEPSQATAGREVKGDVLYWEGEELV
                     VKDISGREVRIRVTADTKIQGVSGRLKTGDKILAQVPPEGPAVSITLQIPDTGPPVR"
     gene            15820..16173
                     /locus_tag="VFR82_12735"
     CDS             15820..16173
                     /locus_tag="VFR82_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502510.1"
                     /translation="MQSPIPSGMTCWLLLSVGVLPAAAQSDRFGGPVEFRSPDRPISA
                     PTLRFPEAAGLSSDSSKLSSTSTTGMSAPENVLKHIDIELGRTLSRSEPGRRGPPDKT
                     DDQREERAQAKGTFK"
BASE COUNT         3472 a         4933 c         4634 g         3241 t
ORIGIN      
        1 gggccccttc caagagcaac atctcgaaga gccgtcgatc gtcatgaacg ggtttccccc
       61 attccttgtc gtgatagcgc cgcatgtgag gctgtgaacc agcccaggca caccgacgct
      121 cgatgcgcgt atctttagac attgtgttgg atcccaaggg aaatcccttg cggtaagttc
      181 acgaggacgg tcgacagcat cggttttcct gtgcacgctc cctgccgact ggtctctttc
      241 ctagcgtgaa cattttcgac tccatcttat agtagaaagg acagttcgtc ttcgcgactc
      301 agtcgtcggg tctcaagaca ctggcgaaac aggtgcaatg aacctggtgg tgcgcataga
      361 aatgaataga cggattgtac ctcgggtctc tgctgtcgcc gatggccaga ggggttgggc
      421 atttcagcgc aatccttcag actccgcaac accgggtgat caatttgtcg actcacgaag
      481 gagggaaacg atgaaatatc atatgatatc tgcttgcctc gcgatggttg cgctcacagc
      541 atgtagccat accatacctg agacctatac caaaccccag gcgccgcccc agttgggcaa
      601 gaccccgccg gtcattcaac ccgtgaatgc cgcgcaaaag caggcgcagg ctccggaatc
      661 gctgctcccc gtcacgatga cggttgacct cacgccggtg caaaagacca tccaagccgc
      721 gctgccggaa cggtttaccg aagaaggtca tccgttggcc aaggactatc ggtggcgttt
      781 catccgggag ggcgagcccc aagtgcagat ccaggacggt ttggtgacat ttagcgccac
      841 ctatcgaggc gagatcgaat ccgctgctgc ccgcgcctgc cgactcgatc ccttataccc
      901 ggttctcgaa ggaacaggaa ggctcgagtt gcatgagcag gaccaggggc tgctcgtcac
      961 catgagtcag ccgcagacgt cgatgagttt gaagccggaa agcgatacga agtgcaatat
     1021 gttcaacctg ccggtcaaag atcagttggc cgaactcttc aaacaggaat cggtgaaaca
     1081 acacatcacg cagtcgctcg agcaggccgg ctatgtcatt ccgttgaatt taatttggga
     1141 ccgactacaa gaacccctgc cggtcagttc ggggaatacc aaactttgcc tctacgggaa
     1201 agccaaggac tttgccatcg gctccctcaa aggtccggtg cagaatacga ccatcacggg
     1261 cgtcgcccgg caaacaccgg tcgcgctctt ccagacccct tgccaaaaac caaccaacgc
     1321 accgttgaag gtgcatatgg acacggccgc aacggccatg caggaaggtc agccctacag
     1381 aatcctcttg agcgtgccgg ttccctacgc cgtcctcaat cagcaactcc aggagcggtt
     1441 gtaccatcag ggagtgaagc ttccgaccac cttcgacaat gacctgacga tcgaacgggt
     1501 gaccgcctct gacgtcagcg gtcggacgct gttcgccgtc gaaaccagcg gggacacgaa
     1561 ggggacgctc tactattggg gcacgcctca gttggagcaa gaggggaata tgatcacgct
     1621 ccctgatctc caaatcgcca acgaaacaaa gattgcgctc gaagagatta agtccggcta
     1681 ttggcagatg gtggatgatc aactgaagcc gcgcttgcga caggcctcaa ccctcgatct
     1741 ctcacagcgg atcggaacaa tgaggagtgc gctgagcggc caacataaat ccggaagctt
     1801 ggccatggat ctgctagtcc ccaagcagga agcgggacag gtggtgtcga ccaaggatgc
     1861 gctcgttgcc gacgtgttcc tggaagggac cgcgagcgcc gcaggacgcc tgcccgtcaa
     1921 gcaagcttca cgcagtgaca ttgatcagag tcatccggtg ggcacggacc gaactccggc
     1981 ggcggaggct gcgccaaaag ccgcccgtaa tcccgacgaa gatcggcgct aacagaatgt
     2041 ctggccgctt tcggccggcc taagcggcca gacgtttcgc tgtcgcacac agcctacata
     2101 tttgagaagt cctgcaggct gtcgaaaaag cgctcaccgg cccaaccccg gcgcaccaga
     2161 acgcgccgtt tccgcgcaca gggccgcagt gaacgcgaag gcgaggcgtg tgcgtttcgg
     2221 tatcgagctt tgcgagcttt gatggatgcg agaacgccgc tggacgttat tcacatgcct
     2281 gcgactacca tgcataggct tccggcgccg gaccgccagg gcccggaaag atcgcatcca
     2341 gccgcttgag tgtctcggta gccaacgtca gattcatcac ccgcatcgta ctcgccagtt
     2401 gctcgagcgt gcgcggcccg atgattggcg aggtcaccgc cggctgatgc agcagccacg
     2461 ctaatgccac gtcggccggc cgttcactga gttcctgaca caacttctca taggcctcca
     2521 atctcggccg atacttttcc acctcctgct gcacatcctt gtcggcacgg cggcccacgg
     2581 tggcgggcgt caacgcaccg gccagcaatc ccctggcaag cgggctccag ggaatcatac
     2641 cgatgccata ggcttcacag gccggaagca cttcaagctc gatcgtacgt tcattcaaat
     2701 tgtacagact ttgttcggag acgatcccca ggaagtgacg gctgcgcgcg agttcttggg
     2761 cctgcgcaag ttgccagccg gcgaagttgc tggtcccaac ataaagaatc ttcccttcac
     2821 gaaccagtag ctccatcgct tgccaaattt cttcccacgg cgtgtcgcgg tcgacatggt
     2881 gcatctgata gagatcgata cagtcggtct gcagacggcg caggctcgcc tcgcaggcgc
     2941 gtttgatgtg cagtgccgac agacgggctt ggttcggcca atcgcccatc cgcccataca
     3001 gcttcgtcgc caacacgacc ttttcccgac ggccgccgcc ttgcgcgaac caacgaccga
     3061 taatctgttc ggtccagcct tccccgactt tccacccata gacgttcgcc gtgtcaaaaa
     3121 aatttatgcc cagctcaaga gcccgatcca tgatggccgc gctgtcggct tctgtcgtct
     3181 cggggccgaa attcatcgtg ccgaggcaca gccggctcac cttcacgcct gatcgaccca
     3241 ggtgaacata ttgcatcaag actcctgctg cgacaaggcg tgttaaagtc cgatcgtttc
     3301 gatccgctgc agctcgtccg gcgtaagcgt aaaacggtcg cactgcagat ccgccgtcat
     3361 atgctcacga ttcgtcgtgc cggtcagggg cagcatgccg acctgcatcg cgaagcgaaa
     3421 cacaatctgg gccacgccgg taccatactt ctccgccata gcgcgaatgc ccgggtcggc
     3481 aaacacttcc cgattggccg tcagcagaga aaaaccctga tacacgatct gttgggtccg
     3541 gcacagatca cgtactgcct tgtcccatcc gaacgcggcg tagcagcggt tttggaccac
     3601 catcggccga tgtgtcgccc gttcacacaa cagtgccagc tggctggccg tcacattgct
     3661 cacgccgatg agcttggtct tgccgctggc atacaatgac tcaatggccg accacacttc
     3721 ccaatcttcc ggccccaatc cgccgcgaga gtacggcccg tggaggacat aggagtcgag
     3781 gtaatccgtg cgcaggtggg tgagtgaact ctcgaacgat tgccggactt gcgtcgtaat
     3841 cggcgctccg gcgtcgtagg gagtccgatg gtcctgccca ttgacgggcg tgaatttcgt
     3901 ttggagaaat aaccggtctc tcgtatgccc gccttgcgcc acgctcaata aggcctcgcc
     3961 cacccgcgcc tcgtcgtaat ggatgagctg gttggcagta tcgatggccg aaaagccggc
     4021 ggtgatggct tcaatgacga gctgggtcgt cgcctccttc ttccaggccg ttccatacat
     4081 gaaggccggc attggcacgc cattgtatgc ggaaagcgtc atcgctccat gtgctccaac
     4141 tgggacagta tgatcaccac tcgttgaatg tgagagagta ccttgcccac catacacaga
     4201 ttcctgtcgc agtctcaatg acggaattgg tacagtgcct cggatggagc ctgagagtca
     4261 gatcgtcgag ttgcggttca gctatcgtta cacgaagcaa cacacctggg tggtgcaggc
     4321 cgtgaatgga ttcctgtcgg cctatttcat ggagcagccc ggctttcgcg tccagcgaca
     4381 ttttgacgag ctggaatccg gcatgcatgt ctggctctgc gaagtgccgg cgaccatgaa
     4441 gatgacccag ctgctcaaac gtctgagggc ggacattccg ccctttcgcc aaacggagac
     4501 acggtcttcc accgacagct gtgcacagta tgtcatcgac tcaatagaac ccgactcgtc
     4561 agcataagac ctcttcgcga aaatttctcc gagacctgtt caaaccggcg gacaagagta
     4621 tactaatgta aaagggaggc ttgggcgatg catcgggtcc tggtcctcgg cgcggggaag
     4681 atcggctcgc tggtcgcctg cctgctatca caacaggggg cctatgaggt ccacctcggg
     4741 gacgtgacgc tcgatgcacc gaagcatctg gtggaagacc tcggcctctc ccgtatcacc
     4801 cctgccgtac tcgatgtgcg ccatcccgat accgtcgaga cgtatctatc cgctcacccg
     4861 gtcgatgcca tcatttccag cctcccgtac ttctgtaatc ccaccgtcgc ggcgcttgct
     4921 ctgaagcatg ggtgccatta tttcgacctg acggaggacg tggaggtcac gaatcacatc
     4981 cgcgccctga gtgcaggagc agcacgcgcc ttcatgccac agtgcggcct ggcacccggg
     5041 tttatcagca tcgtgacaca cgaactcatg acccatttcg aaacgttgga tcacgtcaaa
     5101 atgcgcgtcg gcgccctgcc tgttcatccc agcaacgcgc tgaagtattc gctgacctgg
     5161 tcgacggatg gcctgatcaa cgaatacggc aatctctgtt acggaatcga agacggccga
     5221 aagattccac tccagccttt ggaaggctac gaaaccatcg agctcgacgg attgttatac
     5281 gaggcattca atacttcagg aggattgggg acgctcgccg acagctcggt cggctcggtg
     5341 aagaccatga attacaaaac gcttcgttac cccggtcatt gtgaaaaaat ccagctcctg
     5401 atgaaagacc tcaagctcaa cgaggatcgt aagacactca agcgtgtcct ggagcatgcc
     5461 ataccgcaga cgctccagga tgtcgtgctg atctatgcgt cggtcacggg cacgaagcac
     5521 ggcgagtttt tcgaagagaa ctacgtcaag aaaatctatc ctcaatgcat caagggcaaa
     5581 ttatggtcgg ccattcaggt caccacggcc tcgagcgtgt gttgcgtcat ggacattgtt
     5641 ctgagcaatg gggcggccta ccagggcttc gtcacgcagg aatccatcgg gctgcaggac
     5701 tttttggcca acgacttcgg agcgtgtttc cgatgagcac cgtgcacgca gcgctaaaag
     5761 atttgggaat ccaggccgtc agctccggcg gcagcacggg caagcactgg tggtcggacc
     5821 atgcgaccgg ttccctgatc cagtccgtga acccggcgac cggtgaagcc atcgctgcgg
     5881 tccgttcatg ctcggcagcc gactacgaac acatcgcgac tcaagcacgt gacgtgtttg
     5941 ccacatggcg catggtaccc gctccgaaac gaggggaact cgttcgtctc atcggacaag
     6001 ccttgcggag caagaaggat caactcggca cactcgtgtc ccttgaagtc ggcaagatca
     6061 aggccgaggg agacggagaa gtgcaggaga tgatcgacat ggccgacttt gccgtcgggc
     6121 aatcacgcat gctgtatggc gcgacgatgc agtccgagcg accgtctcat cgcatgagcg
     6181 agcagtggca tccgctcggt cccgtcgggg tcatcacggc gttcaatttt ccggtcgctg
     6241 tgtgggcatg gaatgcattc ctcgccgcca tcgctggtga tccggtcatc tggaaaccct
     6301 cgcccaaggc gccgctgtgc gcgattgcca cccagcagat ttgtaaccag gtcatggaac
     6361 aacaagggta ttccgggatc ttctcgctgt tcatcaccga tcaagcgccg cttgcggaaa
     6421 ccatggttcg ggatccgcgc ttgccattga tctcgtttac ggggtccgta ccggtcggcc
     6481 gacacgtggc gtccatcgtc gggcaacggt tgggtcggac gctgctcgag ctcagcggca
     6541 ataacgccgt catcgtggat cacagtgcag atctcgacct ggcggtacgg gcgattgtct
     6601 ttggagcggt cggaacggct ggccagcgct gcaccaccac gcggcgtctg ctcgtgcacg
     6661 agtcatgcgc tgacgcactc cttgaccggc tagtgaaagc ctataagcag gtgcgcatcg
     6721 gcaatccatt ggattccgac gtcctactcg ggccgctcat cgatcacgtg gccgtcgaag
     6781 cctaccgctc ggccatcgac gaaatcaaaa aggaaggcgg agagatcctt tacggcggcc
     6841 gtgtcctgac aagacccggc tatttcgtgg aaccgacaat cgtccgggcg cagccccact
     6901 ggccgctttt gcaacgggaa actttcgctc ccatcctcta tgcgatgacc ttccggacgc
     6961 tcgatgaggc catcgccatc cagaatgggg tcccgcaagg tttgtcgtcc gccatgttta
     7021 cgaacaatct gctccacagc gagcgcttcc tgtcggggtc agggagtgat tgcggcatcg
     7081 cgaatatcaa tctcggcacc tcgggtgccg agataggcgg cgcattcggt ggagagaagg
     7141 aaacgggcgg cggtcgcgaa gcggggtctg acgcgtggaa agcctacatg cggcgtcaga
     7201 ccaacacggt aaattggggc acggatctgc cgctcgctca gggcatcaag ttcggatgat
     7261 cggccttttg gcaaaaggac actgtcatgg agacaaatcg cgaactcgat gggttactgg
     7321 aacggatggt ggtggaatac gtcgcccgca accgggcggc caagatcctc aaaggtctgc
     7381 tcgacgaggc cggaatcggg ttttaccctg tcgtcgacca cgtcacctta cgcacgatgg
     7441 atatcgaccg gcgagccgaa cccttcctgg cactcggcta cgcctatgat gaaacgctta
     7501 attacgacga ttggtacgcg aaggtctatc gcaaagccgg ctatcccgcc ttgttcgtgg
     7561 atcaggcgta cgcggatgcc cgcgggaaga ccagtatcat ccccggttgg gtcgccgcgt
     7621 tcggagacca gatctttcac catattgccg tgcgggtcga ggacatcgat aaggcggtga
     7681 accggctcaa gacgaacgga gtggtgttcg cggggcaaat cgtcggcccg gcgggcggcc
     7741 agttacggca gattttttcg gcccctgaag tcgtcgatgg gcagcccttc accgtgctgg
     7801 aactcgccga acgacaccgt ggctacttag gatttctgcc gccccaggcc gatagcctga
     7861 tgaaatcaac ggcccctcaa taaatgttac ggggcctctc gactattccc cgcctgcgcc
     7921 gctgcgtctg agttctgcaa gaacgtcggg ccctcgatcg gaagccggat ctcgaacgtg
     7981 gtcccctgcc caggctcgct ttcgacggtg atctgtcctc cgtgcatgtc gaccacttcc
     8041 ttgatgatct ttgtgcccag tccggtccca ccggccttcg tactcactaa ccgatacgtg
     8101 aagagattat cgcgcatgtc tgcgggcatt ccgacccccg tgtcggagat ccagagcgtc
     8161 acgcagtcgt caccggcaga ggcagctccc ttgatcgtga ctgagccttt aggcggcact
     8221 tccgggatgg cgttattcac caggttgtac agcagtgaat acaaacgcgc ctcgtctccc
     8281 acaatcggcg gtaaggtgtg cagcccttcc gtcttcaaga cgacctgttt gcgttcggcc
     8341 ggggcgcgca acgatttcac aacactgtct gcgatctttc cgatcagaca ggattcaaat
     8401 ttttgagaga cccgtgtgag cgctgcgtaa tccgcgatgg ctttcatgcg gtcctgaatc
     8461 cgccgtgacg tattgcgaaa catggtaatg acttcatcgc aggcgaggtg gctttgctca
     8521 gcctgagccg gcgccagcgt cggaagatgt ttgaatacat catcgatctc ttccgcgaga
     8581 aggtcggtgc cggcgacgag gggcatcaag agattcttga cgtcatgggt aatttcaccc
     8641 atcaagcgcg tgatctcgac ccgttgcccc atgcgtgcaa cgagtgtcgt caccgtctga
     8701 tcaatctgtc cctgttcgaa cggcttcgta atgaaatcga cggcgccttc tttcattgcc
     8761 tccacggcga ggcggatcgt gccgtaggcg gtcaccacga cgaccgggag atccggccac
     8821 cgttttctga cgatcttgag cgcctcgatg ccattcagac gcggcatcga gacatcgaag
     8881 aacgccaggt caagggcgtg cgactcgacg gcggccaagg ccgcctgacc gtctgctgcc
     8941 gtcaccacat cgtggcccaa ccaacgaagc cgctcagaca ggctcatgac aatatcgggg
     9001 tcgtcgtcag cgaccagaat gcgaatgccc atggcgctcc cctttcaggt ggtggattga
     9061 gcaagaaatg cgccattggt atcgggcgcc gatgtgacga gaattgcagg aagaattccc
     9121 ggtgaatcct ggcggggttg aatcaattta gacgaagata cggatcaaaa tcgatacgct
     9181 atcccatgtc cttcaacacg tggtccggat tgcggtctcg tgcaatcgtt ttcatgagag
     9241 gatccccgaa caagacgacc acccggccac gctgatcctt gacgatcatc gatgcggacg
     9301 gagccttgaa ggtggcaggg tcgccctcca accacgcact acacgtgaac ggcaaggtat
     9361 cgagcaccgt ctcgacgcga tactcatgac gcaggcgata ctgcaggacg tcgaactgaa
     9421 gccggccgac cgcagccacg agtgattcca agtcattgat gctgcgcatg atctgcactg
     9481 tcccttcctg ggccatctgc tccattccct tgtcgaacga cttccgcttg ccgacatcgg
     9541 tcggacgaat gcgcgcaaaa atctccggtt gaaattgcgg caatggtttg aaacgaaccc
     9601 cccccgtaac ggaaatggtg tcgccgatgg cgaagacacc gggattgata atgccgatga
     9661 tgtcgccagg gaatgcctct tcgaccgtgc tgcgctcttg tgcgacgaga ctgtgaggcc
     9721 gggatagacg tacgtcacga ccaagacgat gatgtttcac caccatgtcc cgctcgaacc
     9781 gccctgagca gacgcggaga aaggcggtgc tgtcccggtg cttggggttc atgttggctt
     9841 ggagcttgaa aacgtaggca ctgaacggtt ggtcgatcgg atcaatggag cgctctcctc
     9901 cgtcgtccgt atccgccaac cgggcaccgg gagcgggcgc cagctgaacg aaagcatcca
     9961 gaaagctctc gacaccaaaa ttcgtcagcg ccgaggcaaa aaacaccggt gtgacctccc
    10021 cgtctaagaa ttgttcgtgc gagagggggt tgccggcaat ggtgagcagt tccagatcat
    10081 gctggacctg tgccatcaca tcaggagaaa cacgcccgct tctggccaag tcctccaatc
    10141 ccatcgtctg cacgtcgact ttcgtggcgc cgccgtgcgc cgttttgctg aatagcgcga
    10201 cgcggttatc ggctcgcgtc acgatgccgg cgaagtcgct tcccgatcca atcggccaat
    10261 tgacggcgct ggcatgaatc gtcaatgcct gttcgacttc cgtcatcaag tccaagggcg
    10321 gacggcccgg taaatccatc ttgttcacga gcgtcagaac cggaatgcga cgcagccggc
    10381 agacggcaaa gagcttgcgc gtttgggctt cgacaccttt tgccgcatcg atgaccatga
    10441 tcgcgctgtc tgcggccgtg agggtccggt aggtgtcttc agaaaagtct tggtggcccg
    10501 gtgtgtcgag cagattgata atggcctctt tgtaggggaa ctgcatcgct gaggccgtga
    10561 tggagatgcc ccgctcctgc tccataccca tccagtcgga ggcggccgtt tttccacctt
    10621 tgcgcccccg aaccatgccg gcggtccgaa tcaggccgga gtacaagagg agcttttccg
    10681 tgagggtcgt cttcccggca tccgggtggc tgataatggc aaatgtccgt cgcttggcag
    10741 tggcaaccgc caactcgttg cgctcagtcg tatcggtgcg catcacggcg cgcagcataa
    10801 cacatccctg tgaggaggcg ggaggcggac agaggaaacg cctctagcct tggacgacgc
    10861 ggttgacaga accgatcgta tggaagagga taccgcctat ccgcacccaa tgaggccgta
    10921 catggagccg aggatcgtct tcggtgctgt gataaggctc agtcgctccg gcacagaata
    10981 atgccctcac gggatgagtc gtccgtgagg gcgttttcgt ttcaacaccc ttctctacaa
    11041 ttgcgtgctc acccctggac aaggaggagt gtatgagact ggtactcacc ctgtggcttg
    11101 ccttgtgcgc ctggtggcca accctcgccc ttgccgaagg cgcgggtggg ggttatcgag
    11161 gcatcggttc catctactac acgttcatct gggccgtgct ttgctacggc ttgtacgata
    11221 ctttcgggaa aaccgtaacg tatacggcgc accgattttg gccgtggcag cctacctcgc
    11281 tctccctccc gggtaggcgg atgtccccgg gccctgacga atgcagcctg tcagggccac
    11341 cgcaccatga acacctgttc aattgccgag aggaaacctt cgctcattga acttgcatcc
    11401 gggatcgtga gtagctgagc acatctcgga agggcgtctg gcttcaccat aagtgagcaa
    11461 aagaggaagc gtgtgtctcg cggtcggatt cgcccatcgg cggcctggac taggccaata
    11521 cctcgttgct gccagcgggg agactctcta gagttctcta ctaagattga cgaggtgctc
    11581 atgaacgtct tgctcgtgca tcccggccca ctcatgtaca cgaaaatata cttgcgactc
    11641 gaaccgctgg ggctggagct cgtcgcctca gcggcacggg cgctcggtca ccgcgtgaca
    11701 atgctcgatc tgcaagtgga gtcgtccaac gactacgtcc gcctactgcg cgaacactgt
    11761 cccgacgccg tcggcttttc gtgcaattac ctggcgaacg tgccggaagt cgtgaatttg
    11821 gccaaactcg ccaaacgctc gctcccccgc tgtgcggtat tcgtcggcgg tcatagtgca
    11881 tcattcgtcg cggatgcgct tctcgttcat ggcgaaggag cgatcgattg cgtgttgaag
    11941 ggcgaagggg agaccgccat cggccctgtg cttgctgcgc tcgccgatcg ggacaacccg
    12001 ccgggtgtga ttcctggcgg catcacgacc gcgggcgagg gaccaccggt ccgaatgctc
    12061 gagcacctcg acgctcatcg gcctgcccgc gacttgctcc gccaccgcag gaaatacttt
    12121 atcggcgtct tggatccttg cgcctcaatc gagttcaccc gtggctgtcc atgggactgc
    12181 tctttttgca gcgcgtggac cttttacggt cgcagctatc gcgtccgtac gcccgaagcg
    12241 attgttgaag acctggggac ggtccgcgag cccggcgtct ttatcgtcga tgacgtcgca
    12301 tttatccagg ccaaacatgg tttggccatt ggcgaggcaa tcgcccggcg aagcataaag
    12361 aaacgctatt atctggaaac acgaggcgac gtgctcttgc gaaacaaaga agtcttccgt
    12421 atgtggaaga cccttggact cgcctatatg tttatcggca tcgaggcaat cgacgaggaa
    12481 gggctggcac agcaccggaa acgcattacg ctgagcaaga attttgaggc gcttgaattc
    12541 gcgcgctccc tcgggatcac ggtcgccatc aatctcatcg ccgatccgtc ctgggatcgc
    12601 cgccgtttcg agatcgtgcg ccagtggtgc ctcgaaatcc cggaaatcgt caatattagt
    12661 gtcaacactc cctatcccgg cacggagacc tggcatacgg aagccaggca cgtcacgaca
    12721 cgggattacc ggctctacga tattcagcac gccgtcctcc ccactcacct gccactgcct
    12781 gagttctatc atgaactcgt gcagacgcag cagattctca atcgtaaaca cttgggttgg
    12841 aatgccttga agtcgaccgc ccgcatcgcc gcaggccatt tactccgagg gcagacaaat
    12901 ttcgtgaaga tgctgtggaa attcaacagc gtctacgatc ctcgtctcca actggcggat
    12961 cacgcgcgtc cggtgctcta cgaaatgccg ctgcccccca agcggacatc cggcatgcag
    13021 acggacttgc tctatatcca ccgcgcacgg ggccgcgctg gccgtgtctt ggacgacgcc
    13081 acggagcaat tcgtcgaaac gacgcgcatg ggaacgaccg cctgactgtg tgccgtcata
    13141 cggtcgagcg aaacccgcgc cctcacgaac gttgcccggc ctgaccttgc cacagcatgt
    13201 catagcccag ctccttggtt tccacacaca tggcggcaag ggcggagaat tttttggtga
    13261 acagctcgtt cgcgtgagag cgctcatgat cctcgaacga tctccagtag gtcacaatca
    13321 cgtaatgatc ctctgcggtc ttcgatgacg aaagtgaacc ctcctccgag acaaaaccgg
    13381 aaaatttgaa aacctgcccc gcgagaaacc cgcctttgtc tccaccgtac gtgtctttga
    13441 cgacgctgca cagctcgcca acggccaatt cgatatcctc gaaggtgacg ccgggtttga
    13501 gcgtaaccgt attgaacaac attttggctc cgaatggcag tgtgatgggt ccgaacatga
    13561 taattcctct cctctcattc aggccgttag gcgtgcacgc gacgctcgcc gatcccgagg
    13621 cgtaccgcct caatgtccaa ttcatgctcc agacggtgga ggacttcgtc gctgatcgtc
    13681 ccgtcattcc gcataccgat taatgccatc cgttcggcag taagcgtctc atgacggagg
    13741 cgccgaaagg cttcggctga ctccagcgaa cattccggat ccacgtggcc gttacctcca
    13801 aagcgttgca tccggcggcc ataatggtct cgcagccgtt caacctgctc tccgatggac
    13861 caggactcgt cagccaatgc ctccaaccgt gtcagggcca tcgtggccgc atgttcgcgc
    13921 gcgagcattt cctcctgctc gaaccctcga tcctcttcaa gatgcaaggc ccggatgagg
    13981 ggggtgagcg acagcccctg cagcaccagt gtgaccagga tcaccgtaaa actgatgagg
    14041 atgatttccg ctcggaaggg cagcggtgtt ccggctgcgg tggcgaccgg caacgcgagg
    14101 gccgcggcaa gggtgacaat gccgcgcatg cccgtccagg cgaccagaaa gatgttcggc
    14161 caggcaggaa gcggatcgcg cgcccgcagc gacgcgctca accagcgtgg aatcagcgca
    14221 gcgagcggca cccagccaag acggacaacg atggccgtga ggctgacgac gatgccggtc
    14281 aggagaaccg gggctaggcc gcccggggga atcgcctggc tcaacgcacg aagttccaaa
    14341 ccgatgagga tgaagatgaa tccgttcaac acaaacacga gcacatccca gaccgcgcgg
    14401 ccttgaatcc tggtgatcgg cgacacggcg gcactgaaat gctgtcgaag atgcaagccc
    14461 cccgccacac aggccaagac ggccgacgca tgaacctgtt cccccaatac ccacgctaca
    14521 tatggagcga gcagcgtaat ggcaatttcc gtaaagctgt catgagtagc acacaacgcc
    14581 cagcgtgcgg cgatgctcac ggcgattcct atcgcgaccc cgacgctggc cgcaaacaca
    14641 aacgtcacga gtccttctcc aggaacgaaa ctccctccga tcgcgacccc cactgccatc
    14701 cgatagacga ccaatgcggt cgcatcgttg accagacttt cgccttcgag aatcgtgacc
    14761 acccgacgag ggatgcgcag ccgctttccg atcgcggtgg ccgagaccgc gtccggtggt
    14821 gacacgatag cgccaagcgc aatcgcctcc gcccatccca tgccgggcag cagccagcgc
    14881 gccacgatcg ccaccgcgcc gcttgtcaca accactaacc cgatcgcgag cagcgaaatc
    14941 ggccggaggt tgcgacgaaa ctcccgccag gacgtgaaat atgcggccgc ccacaaaatc
    15001 ggcgggagaa agaccaggaa gacgagatcc ggattcaagg tgactgtcgg gacgcccggt
    15061 ataaggctta ggatcaatcc gccgatgacc aggaggatgg gataaggtac caccacaatc
    15121 ttctgcgcga tcgtcgtaag agcgagcacg acgcccagca gaagaatgac gatttcgagc
    15181 tggtgcaatc cttccataag agtctcgaca atcagcttgg agcgctgtgc gtgagtaggc
    15241 tacgtgggag gcctcgagga gtcaatctgg cccgagaaag gagagagtac cgctcgatga
    15301 atagagcaat gccgggcagg catgagtcac aagctgccaa cacggtcaag ggcgatcagc
    15361 ggacaggcgg tccggtgtcg ggaatctgaa gggtgataga aactgccggc ccttcaggcg
    15421 gaacctgcgc caaaatcttg tcccccgtct tcagtcgccc ggacacgccc tggatcttcg
    15481 tgtcggcggt cactcggatg cgtacctccc ggccggagat gtccttcacg accagttcct
    15541 ctccttccca atataagaca tcgcccttaa cctccctgcc ggcggtcgcc tgagatggtt
    15601 ccgcgaaagc cgagtcaacc gttccggcac acaacagcca taacagcaac gcgagtgcca
    15661 taggctatcc tcctccctgg tttcagccta gcaatcattc ctacccttgt cagtttactg
    15721 ggataaacct ctgtgcgcaa ttgcaggtca gggaaccctc gaagcaaatc tagggaaagg
    15781 acgagagcct cagtggcgca aaaagagcga ttgttcagca tgcaatcccc tataccaagc
    15841 ggcatgacct gctggcttct cctgagcgtc ggtgtactgc ctgctgcggc gcagtccgat
    15901 cgctttggcg gacccgtaga attccgttct cccgatcgcc cgatcagcgc cccgacgctc
    15961 cgctttcctg aagccgcggg tctgtcctca gattcaagca agctctccag tacctccact
    16021 accggtatgt cggcccctga gaacgtgctc aaacacatcg atatcgaatt gggtcgaacg
    16081 ctgagtagat ccgaacccgg ccggcgaggt ccaccggata agaccgacga ccagcgtgaa
    16141 gagcgcgcgc aggcgaaggg cacgttcaag taacggtgct gtgtacacac cccaacatct
    16201 ctggaaggtc ttcactcgtg acacgcgtga gctgttcgat cggctgtgat cgcctcggac
    16261 accctcgatt cagtgaacga
//
LOCUS       DASTQY010000390         3076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_518448, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000390 DASTQY010000000
VERSION     DASTQY010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518448"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VFR82_12740"
     CDS             <1..909
                     /locus_tag="VFR82_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502511.1"
                     /translation="LFIDQAKSGDPQMDGVACSQCHLIKQVDRTKQPPEPKYEPAGSK
                     TLYGPYKDFAQNLAHQSLELGLFRKSDLCLNCHQAVPSAANLGKSNDLLGNWDQSKAI
                     KSGKECQSCHMPEQTGESANGEKKRTVANHTFPGRIGKLRQEAAKLDVETKIEGEKTT
                     VVLKVQSLVPHNLPTTHPAWASVVLQLDIKGKNLKTVFSDKRVYGRVFQDAKGQKTVF
                     DFEAVKVLEDTVLKPEELRVETFTFPTPKDTRTFDVEATLSYAPVSGPASFLQRIEAE
                     SSKGSSDPVFQPIDIVKKTENIPVNK"
     gene            1037..2389
                     /locus_tag="VFR82_12745"
     CDS             1037..2389
                     /locus_tag="VFR82_12745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU4502512.1"
                     /translation="MRLSKTSVIVVGAGLAGLSAAVRLMKDGAHVTVVEARDRVGGRV
                     LTIRDAFARGQHAEAGGDFIDDSQEDIRRLADQYGLTVRTILRQGFSFVRDKGKGQIH
                     GRPLKGERAWKPIVDRAKPLIEAYRRAEQRWESAVVRSMAQRSVAQWLDEIDADGNMR
                     AMVRGLRGFFLADPEDLSLLVLIEQLASEAPGQEAMGRIEGGNDRLPRVMAVELGESL
                     HLNTAARAIHDDQPFAHVTVESAGGDRTQIKADYVIVSVPATALRAIEITPDLPLEQA
                     KVFERLKYGRVTKSLLQFDRRFWKRKGRSPAYGTDAPTGAIWDANEEQRGSAGILTLM
                     AGGQASEDTQKILAQDGVEGLVRALEWLKPGDAELLHSHHVMWEHDSWSQGGYAYFDP
                     SFDPAWRPWLARPHGRLLFAGEHTSIKWQGYMNGAVESGLRAAAEVWALAAAGNRRAS
                     "
     gene            complement(2396..2650)
                     /locus_tag="VFR82_12750"
     CDS             complement(2396..2650)
                     /locus_tag="VFR82_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502513.1"
                     /translation="MGLEPNYIVIDGQTFSKTKLALDNHVYKNCNIDDCDLYYSGGQY
                     ELLDTHITNSRLILNHPAKGIYNALQIFKMKSPGSRIVYE"
     gene            2762..>3076
                     /locus_tag="VFR82_12755"
     CDS             2762..>3076
                     /locus_tag="VFR82_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502514.1"
                     /translation="MPERLCSRPLPWQLWVGLLALTGCAEVSPNASEPFPAQRALVGA
                     TEQVLLACAGQPVIRRTKGEEVLFIYYREASQFEESFGGSKSSFPMVHHGCRATIRLQ
                     QDR"
BASE COUNT          632 a          879 c          971 g          594 t
ORIGIN      
        1 ctcttcatcg atcaggcgaa atcgggcgat ccgcagatgg acggtgtggc ctgctcccaa
       61 tgtcacctca tcaagcaggt ggatcgcacc aaacagccgc cggagccgaa gtatgagccg
      121 gccggcagca aaacgctcta cgggccatat aaggattttg cgcaaaatct tgcccaccag
      181 tcgttggagt taggactgtt ccgaaagtcc gacctctgct tgaactgtca tcaggcggtg
      241 ccgtcggcgg caaacttggg caaatcgaac gatttgctcg gcaactggga tcagagcaag
      301 gcgatcaagt caggcaagga atgccagtcc tgccacatgc ccgagcagac cggcgaatcg
      361 gccaacgggg aaaagaaacg caccgtggcc aatcatacat tcccaggtcg catcggcaag
      421 ctgcgccagg aagcggcgaa gcttgatgtc gagacaaaga ttgagggaga gaaaacgacc
      481 gtcgtgctga aggtccagag cctcgtcccc cacaatctgc cgacgaccca tccggcttgg
      541 gcgagcgtcg tgctgcaact ggatatcaaa ggcaagaatt tgaagacggt gttttctgac
      601 aagcgcgtgt atggcagagt ttttcaggac gccaaagggc agaagacggt cttcgacttc
      661 gaagccgtga aggtgctgga agataccgtc ttgaagcccg aagagctgcg cgtcgaaact
      721 tttacgttcc ccactccgaa ggacacgcga acgtttgacg tggaagccac gctcagctat
      781 gcgccggtca gcgggccggc atccttctta cagcgcatcg aagccgagtc ttccaagggg
      841 tcttcagacc cggtgttcca accaatcgac atcgtgaaga aaaccgaaaa cattcccgtc
      901 aacaagtagt ctgtctgccc aggctgcttc tccaatttgt ctttccaaac aatgagttgg
      961 gagccaggct ccttcaaagc gtgagctagg gaacccggcc ctggcggccg gacagagggg
     1021 ggagtaggct gtccccatgc gtctcagtaa gacatcggtg atcgtggtcg gcgctggcct
     1081 ggccggactt tccgctgccg tccggttgat gaaggatggg gcgcacgtga cggtcgtgga
     1141 ggcgcgggac cgcgtgggcg gaagggtgct gacgatccgc gacgcctttg ccagaggcca
     1201 gcatgcggag gcgggaggcg attttatcga cgacagccag gaagacatca ggcggctcgc
     1261 ggaccagtac ggcctgaccg tgcgcacgat tctgcgccag ggattttcct tcgtgcgcga
     1321 caaggggaaa gggcaaatcc acggccgtcc attgaaggga gagagggcgt ggaagccgat
     1381 cgtcgaccgc gccaagccac tgatcgaggc ctatcggcgg gcggagcaac ggtgggagag
     1441 cgccgtcgtc agaagcatgg cgcagcgatc ggtcgctcaa tggctggatg aaatcgacgc
     1501 cgatgggaac atgcgggcga tggttcgcgg cctgcgcggg ttcttcctcg ccgatcccga
     1561 ggacctttcg cttttggtcc tgattgaaca gcttgcatcc gaagcgccgg gacaggaagc
     1621 gatgggacgc attgaagggg ggaacgatcg attgccgagg gtcatggccg tggaattggg
     1681 cgagtcgttg caccttaaca cggctgcccg cgcgatccac gacgatcagc cgttcgcgca
     1741 cgtgacggtc gaaagcgccg gcggcgatcg gacgcagatc aaggcagact atgtcattgt
     1801 ctccgtgccg gccactgcgc ttcgcgccat tgagatcacc ccagacctgc cgcttgagca
     1861 ggcgaaagtc ttcgagcgat tgaagtacgg ccgggtcacc aagagtctgc tgcaattcga
     1921 ccggcgtttc tggaagcgca agggccgttc tcctgcctat ggcaccgacg ccccgaccgg
     1981 cgccatctgg gacgccaatg aagagcagcg aggctccgcg gggatcctga cgcttatggc
     2041 tggcggtcag gcgagcgaag acacccaaaa gatcttggcc caggacggcg tagagggttt
     2101 agtgcgcgcc ctggagtggt tgaagccggg agatgccgag ctgctgcaca gccatcatgt
     2161 gatgtgggag cacgattcgt ggtcccaggg cggctatgcc tatttcgatc cttccttcga
     2221 tccggcctgg cgcccttggc tggcccgtcc acacgggcgg ctgctgtttg ccggtgaaca
     2281 caccagcatc aaatggcagg gctatatgaa cggggcggta gaaagcggtc tccgcgccgc
     2341 ggcggaagtg tgggcgctcg cggccgctgg caaccgtcgc gcctcctagc ctgcgctatt
     2401 cgtagacaat cctcgaaccc ggcgacttca tcttgaagat ctgaagcgcg ttgtagatac
     2461 ctttggcagg atggttgagg atcaggcgtg agttggtgat gtgtgtatcg agcagttcgt
     2521 attgcccgcc gctgtagtag agatcgcaat catcgatgtt gcaattcttg tagacgtggt
     2581 tgtccagcgc caacttggtc ttgctgaacg tctggccgtc gatgacgatg taattcggct
     2641 ccaagcccat ccggtttctc ccgcgcggac tataacaaag tcttctccgc tccgcaaacg
     2701 cgtgactgct gcgattgcgc cttttctttc accttttcct agggtattct tcggcatgcc
     2761 catgccggaa cgactgtgca gccgacctct gccatggcag ctttgggtag gactcctcgc
     2821 cctgacaggc tgtgcggaag tgagtccgaa tgcctccgag ccgttccctg cccagcgggc
     2881 cctggtcggc gcgaccgaac aggtgctcct cgcctgtgcc ggccaaccgg ttatccgacg
     2941 cacaaaaggt gaggaggtgc tcttcatcta ctatcgggag gcctcgcagt tcgaggaatc
     3001 gtttggcggc tcgaaaagca gttttcccat ggtgcatcac ggctgccgcg ccacgatcag
     3061 acttcagcag gaccgc
//
LOCUS       DASTQY010000391         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_876415, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000391 DASTQY010000000
VERSION     DASTQY010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876415"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFR82_12760"
     CDS             complement(<1..239)
                     /locus_tag="VFR82_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502515.1"
                     /translation="MLSHAQLLAAFRSQITRLLQERDTQWERSRRLIEAASFSGTVRS
                     LLERAQHTDLPAPVRDALLTALNEGQVARIQDLSGP"
     gene            401..1369
                     /locus_tag="VFR82_12765"
     CDS             401..1369
                     /locus_tag="VFR82_12765"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEU4502516.1"
                     /translation="MIGHGLLIGLLWMTAGCAATDASVSPTPPSTEFREGQIIDTANG
                     QFISLEQLVSHLLQQDVVYLGEEHHNRFHINSALSVLNGLSAAGRRPVVAMEMFGWDA
                     QPLLDRYVGGGELSRQEFLSQVGWSQNWGGAFETYEPLVAFAKHERLGLVALNPPKSL
                     VRLVAREGLDHARQEADWKRWKMQDETIVDDPLYRQRIFDQLRACHGGGSDDMYRTMY
                     EASMVRDEGMARTVVALVESIRERSDALAGPVVSYTGGGHVQYNLPVANRVVRRLSGN
                     VRQTSLYMTSYERGRQDDVREMLDGKIADYVWLTPVSGEGMPRRCR"
     gene            1489..>2160
                     /locus_tag="VFR82_12770"
     CDS             1489..>2160
                     /locus_tag="VFR82_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502517.1"
                     /translation="MRNKIPCSAERLSLLDPPMIQGVVDRNAFHLGSQYLSENRVRIV
                     EANEAQISSAVIGNSGLYEQTIRLKDGHLVSRCSCSLPEEPMCRHCIAVLLEYHRWVQ
                     PRTKKARQNPVPMSSEASNGNTVPPSNFTADLKLSEVMAFIEWLNPTMKALEKSEPLP
                     EQSHLSGDLAAWVQTIRTRDERRRESEELQLTLESEQREREAYVERMTQQLQASMAEV
                     KAAQSQ"
BASE COUNT          457 a          570 c          677 g          456 t
ORIGIN      
        1 ggtcccgaca ggtcctggat cctggcgacc tggccctcgt tcaaggctgt aagcagtgca
       61 tctcgcacag gcgcgggcaa atctgtgtgc tgggcacgtt cgagcagact gcgtaccgta
      121 cccgagaaac tggcagcttc gatcagacgg cgcgagcgct cccattgcgt atcgcgttct
      181 tgtaagagcc tggtaatttg ggagcgaaat gctgccagca actgcgcgtg agagagcatg
      241 aaaatatgga gatgcgatca acaagacggc gtgacttgtc atcacacgcc gggcagcata
      301 ccggccatag accgaagggt caaggacggt cgccttgcgg cctatgccgg aaaaagggca
      361 gaatcggcga gaggatcttc tatcccggag tgcgcatttt atgatcggac atggactact
      421 catcggtcta ctgtggatga ctgccggctg cgcagcaacg gacgcgtcag tgtctccgac
      481 gcctccatcc accgaattcc gagaaggcca aattatcgac acggcgaacg gccaattcat
      541 ttctttggag cagctggtca gtcatctgct ccagcaagac gtcgtctatc tgggagagga
      601 acatcataat cgcttccaca tcaattccgc cttgtccgtc ttgaatggtc tttctgcggc
      661 tggccgccgg ccggtcgtgg ctatggagat gttcggttgg gacgctcagc ccttgctgga
      721 ccggtatgtc ggggggggcg aattaagtcg ccaagagttt ttgagtcagg tgggatggag
      781 ccagaactgg ggaggggcgt ttgaaaccta tgagccgctt gtggccttcg ccaaacatga
      841 gcgtctgggg ttggtggcgc tcaatccgcc taaatctctc gtgaggcttg tggcgaggga
      901 aggtctcgac cacgccaggc aggaggcgga ttggaagcgg tggaagatgc aggatgaaac
      961 gatcgtagac gatccgctgt atcggcaaag gatttttgac cagctccgtg cctgtcatgg
     1021 cggcgggtcc gacgacatgt accgaacgat gtacgaagcg tcgatggtgc gtgatgaggg
     1081 catggctcga acagtcgtcg cgttggtcga gtccatacgt gaacggtcgg atgcgttggc
     1141 cggtcctgtc gtcagctata cgggcggggg acacgtgcaa tacaacctgc cggttgccaa
     1201 tcgagttgtg cggcggttat ccggtaacgt ccggcaaacc agtctttaca tgacgtcata
     1261 tgagcgggga cgacaggacg acgtgcgtga gatgcttgat ggcaagatcg ccgattatgt
     1321 ctggctcacg cctgtgagtg gcgaggggat gccccgccgt tgccgctgaa tcgacccggt
     1381 cctgcgtaga atcgctccgt ccttgacact tttggcatgg cgtttcacac taggggcgtg
     1441 cgttgagagt ttccggcagc gcgtcgttcc cggtgtggga ccatctgcat gcgaaacaag
     1501 atcccctgtt ccgccgaacg gttgagtctc ttggatcctc cgatgatcca aggtgtcgtc
     1561 gaccgcaacg ccttccatct cggcagtcag tacctgtcgg aaaatcgcgt gcgcatcgtg
     1621 gaagccaatg aggcgcagat ctcctcggcc gtgatcggca actccggcct ctacgagcag
     1681 accattcgat tgaaagacgg ccatttggtg tcgaggtgca gttgttcgtt gcccgaagag
     1741 ccgatgtgcc gccattgcat tgccgttctt ttggaatacc atcgatgggt gcagccgcgc
     1801 acgaaaaagg cgaggcaaaa tccggtcccg atgtcttctg aagcttccaa tggaaatacc
     1861 gttcctccat cgaatttcac ggcggatctc aagttaagcg aggtcatggc ttttatcgag
     1921 tggttgaatc cgacgatgaa ggcgctggaa aagagcgaac cgcttcccga gcagtcgcat
     1981 ctgagcggcg accttgccgc ctgggtgcag accatccgga cccgggatga acgacgccgt
     2041 gaaagtgaag agcttcagct gacactggaa tcggagcagc gggagcgcga agcgtacgtg
     2101 gagcggatga ctcaacaact tcaggcttcg atggccgaag tcaaagcagc tcagtcacaa
//
LOCUS       DASTQY010000392         3365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_689019, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000392 DASTQY010000000
VERSION     DASTQY010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689019"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /gene="ccsA"
                     /locus_tag="VFR82_12775"
     CDS             <1..808
                     /gene="ccsA"
                     /locus_tag="VFR82_12775"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEU4502518.1"
                     /translation="FMAAVFFMVTMALYFAATVSFLALLLRRSDALSTFSLTVTAVGF
                     ATHTVALVMRMLTGPGASTPSIHEALSFFSWMLILVFLAVEFRHRLHVLGSFIVPLAL
                     VSLVSAAALPETVPTLQPVFRTLWVHVTLSMLGTVGFAVAFVAGVMYLIQDGLLKSKR
                     FNVLYSKLPALDFLDDLNQQSIVLGFPLLTLGIITGAISAEFARGTYVNWNPEQTWAL
                     VTWLFYFCVLVGRLTVGWRAKRAAYLTIIGFAGVVLTLVGVAIKSFGSVS"
     gene            810..2147
                     /gene="hemA"
                     /locus_tag="VFR82_12780"
     CDS             810..2147
                     /gene="hemA"
                     /locus_tag="VFR82_12780"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEU4502519.1"
                     /translation="MHIVVVGLSHKTAPVEIREKLAVPESRLGEALTRLCAYQGIKEG
                     ILLSTCNRVEVYAVVEDLEAGYSRIQEFLADAHLSVSSEQLTPHLYWQTGDRAITHLF
                     RVACSLDSMVVGESQILGQVKDAFETALTHKTTGLILNKVVKKAISVAKRVRTDTRIA
                     ETAVSVSYAAVELAKKIFSDLRDKTVLLVGAGEMAKLAARHFMAAGVRHVLVTTRTPQ
                     HAVVLADQFSGTAVPFEQFREEMATADIVLVSTGAAHYLVGSDDVQRAVKQRMNRPMF
                     LIDISVPRNIDPAVRHVDNAFLFDIDDLKCRVEQNRGERLQEAERAEHMVIEEVAAVR
                     QWLQTLEVTPTIVALKGRVEDIKRAEVEKVLARLGHLPPQERALVEAMASSIVNKLIH
                     NTMVTLKSEVTTADGAAFVEAARRFFNLGDAGPITDEIEVDESTTRTSLNKPG"
     gene            2166..2510
                     /locus_tag="VFR82_12785"
     CDS             2166..2510
                     /locus_tag="VFR82_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502520.1"
                     /translation="MGLATKGPKLFTCPQCRKAYLGHDPLPDCPSCGYDYRAKGGFRW
                     DVLVYLLSVLGMLSYFLVSSSYRSLITESVTTSPGPASQHSTSAPASGTEKLPGVDRA
                     PFVYPPERGADR"
     gene            2512..>3365
                     /gene="hemC"
                     /locus_tag="VFR82_12790"
     CDS             2512..>3365
                     /gene="hemC"
                     /locus_tag="VFR82_12790"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEU4502521.1"
                     /translation="MTGVMHERSTLVLGTRGSKLAVHQSEWVRARIRELAPHLTVTLR
                     RIQTSGDKILDVPLAKIGGKGLFVKEIEEALLSGEIDLAVHSMKDVPTQLPAGLDLLC
                     VPLREDPRDALISRDGRRFKDLPFGAKIGTSSLRRQAQLLQARPDVSISMLRGNLDTR
                     LKKLREGHFDAIVLAAAGLRRLGWEGDITEYLAPEISLPAIGQGALGIEGRTDDAFLR
                     EVLSGLEHAATRTMVIAERALLHRLQGGCQVPIAAHATLNGSEIVLDGLVASVDGKEV
                     IRDRVHGTT"
BASE COUNT          633 a          959 c         1047 g          726 t
ORIGIN      
        1 cttcatggcc gccgtgtttt tcatggtcac tatggcgctg tattttgcgg ccacggtgtc
       61 cttccttgcc ctgctgctgc ggcggtctga tgccctctcg accttctcgc tcacggtcac
      121 ggcggttggt tttgccacac acaccgtcgc gcttgtcatg cggatgctga ctgggccggg
      181 ggcttcgaca cccagcattc acgaagcgct gtcgtttttc tcctggatgc tgatcctcgt
      241 gtttctcgcc gtggaatttc gccatcgcct tcatgtgctg ggatcgttca tcgtgccgct
      301 ggcgctcgtc tcgctggtgt cggccgccgc gcttcccgaa acggttccca ccctgcagcc
      361 ggtgtttcgt acgctgtggg tccatgtgac gctcagcatg ctcggcacgg tcggctttgc
      421 tgtcgccttc gtcgcgggag tcatgtatct gattcaggac ggccttctca agtccaaacg
      481 attcaacgtg ctgtattcga agctgcccgc cctggatttt ctcgacgatt tgaaccaaca
      541 gtcgatcgtg ttgggttttc cgcttctcac gcttgggatc attaccggtg cgatctcggc
      601 cgaatttgcc cgcggcacct acgtgaattg gaatccggaa cagacatggg cgttggtcac
      661 ctggctcttt tacttttgcg tgctcgtcgg gcggctcacc gtcggatggc gtgccaagcg
      721 cgccgcctat ttgacgatta ttggattcgc cggggtggtg ctgacgctgg tgggtgtggc
      781 gatcaaaagc ttcgggtcgg tctcctgata tgcatatcgt cgttgttggg ctcagccata
      841 aaaccgcacc tgtggaaatc cgggaaaaac tggctgttcc tgaaagccgg cttggagaag
      901 ccctgactcg actgtgcgcg taccaaggaa tcaaggaagg catcctcttg tcgacctgca
      961 accgggttga ggtctatgcc gtcgtcgagg atctggaagc ggggtacagc cgcatccagg
     1021 aattcctggc tgatgcgcac ctgtcggtgt catcagaaca gttgacgcct catctgtact
     1081 ggcaaaccgg cgatcgggct atcacgcatt tatttcgcgt cgcctgcagt ctggactcta
     1141 tggtggtcgg tgagtctcag attttaggcc aggtaaaaga cgcatttgag acggcgctca
     1201 cccacaagac cacgggtctg attctcaaca aagtggtgaa gaaggcaatc tcggtcgcga
     1261 aacgtgtgcg cacagatacc aggattgccg agacggcagt ctcggtcagc tatgctgccg
     1321 tcgagttggc gaaaaagatt ttttctgatt tgcgggacaa gaccgtgttg ctcgtcggcg
     1381 caggcgaaat ggcgaagctg gcagcgcggc attttatggc cgccggtgtg cggcacgtcc
     1441 tcgtgaccac gagaacgccc cagcatgccg ttgtcttggc agaccagttc agcgggactg
     1501 ccgtgccgtt cgaacagttc cgggaggaaa tggccacggc cgacatcgtg ctcgtctcga
     1561 cgggggccgc acattatctc gtgggcagcg acgacgtcca acgtgccgtc aagcaacgca
     1621 tgaaccggcc catgttcttg atcgacattt ccgtgccgcg caacatcgat ccggcggtcc
     1681 ggcacgtcga caacgcattt ctcttcgaca tcgacgattt gaaatgcagg gtggagcaga
     1741 atcgcgggga acgcctgcaa gaggcagaac gggccgaaca catggtgatt gaagaagtcg
     1801 cggcggtccg gcaatggctg caaacgctgg aggtcacacc cacgatcgtc gcgctgaagg
     1861 gccgggtcga ggacatcaag cgggccgagg tcgagaaggt cctcgctcgc ttggggcatc
     1921 ttccacctca agaacgggcg ctggtggagg cgatggcctc atcgatcgtg aacaagctca
     1981 tccataacac gatggtgaca ctgaagtcgg aagtgaccac cgcagatggc gcggcttttg
     2041 tcgaagcggc caggcggttt ttcaacctgg gggatgccgg gccgatcact gacgagatag
     2101 aggtcgacga gtccacgact cgcacgagcc tgaacaaacc cggttgacgg cgcaaggaac
     2161 atccagtggg attggccact aaaggtccga agctctttac gtgtccgcag tgccggaaag
     2221 cctatctggg acatgacccg ttacccgatt gcccctcctg cggctacgac tatcgcgcga
     2281 agggcggatt ccggtgggat gtgctcgtgt atctgctgtc ggtgctgggg atgctcagtt
     2341 attttcttgt ctcgtcttcc tatcgcagtc taattactga atccgtgacc acgagccctg
     2401 gacccgcctc gcagcattcc acgtccgccc ctgcatcggg cacagaaaag cttcccggag
     2461 tcgaccgggc gccgtttgtc tatccgcccg aacgcggcgc ggatcgctga catgacgggt
     2521 gtgatgcatg agcgatcgac gttggtcctc gggacacgcg gcagcaagtt ggcggtccac
     2581 cagagcgagt gggtgcgggc ccgaatccgg gagctggctc ctcacctgac ggttacgctg
     2641 cggcgcatcc aaacgtccgg cgacaaaatc ctcgacgtcc cactggccaa aatcggcggc
     2701 aagggcctct tcgtcaaaga aattgaggag gcgctcttgt ccggcgagat tgatctcgcc
     2761 gtccacagca tgaaagatgt gccgacgcaa ttgccggcgg ggttggattt gctctgcgtg
     2821 cccttacgag aggatccgcg ggacgccctg atcagtcgcg acggcagacg ctttaaagat
     2881 cttcccttcg gcgcaaagat cggcacgagc agcctccgcc gtcaagcgca attgctccaa
     2941 gcgcggcctg atgtgtctat cagcatgttg cgggggaacc tcgatacccg attgaagaag
     3001 ctgcgcgaag ggcatttcga tgccattgtg ctggcggccg ccggtctgcg ccgcttagga
     3061 tgggaagggg atatcacgga gtatctggct cccgagatca gtctgcctgc catcggccag
     3121 ggcgcattag gcatcgaggg cagaacagac gacgcctttt tgagggaagt cttgagcggt
     3181 ctcgagcatg cagcgacgag gaccatggtg atcgccgagc gggcgttact gcaccgcttg
     3241 cagggaggct gccaggtgcc gattgcggct catgccacac tgaacggatc ggaaatcgtg
     3301 ttggatggac tggtcgcgag cgtggacggc aaggaggtca ttcgcgaccg tgtgcacggg
     3361 acgac
//
LOCUS       DASTQY010000393         9700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_488981, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000393 DASTQY010000000
VERSION     DASTQY010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9700
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488981"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2052
                     /locus_tag="VFR82_12795"
     CDS             <1..2052
                     /locus_tag="VFR82_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU4502522.1"
                     /translation="VLEYSPEGARAYFATESLKALSSVEHALSGVPLRQVARQIKLVV
                     QGLCGSDVSIIARPEAFTTPIIRPIVSADGKTIAMPALLRRYPTARQNERLYLVMAAH
                     EAGHLEFGTYTLPISSLLDLLETVCGRYGRPRPAALESLAALFRLYPHPRLMQDLWTI
                     LEDARVESFLQREYPGLQKDLAQFAADTILPRDPAHGLMVRELIADCLLRLSVGESMS
                     SAVPHAVTEEVSILWNLCQPVLTPDSTAERSVRTAHEVYVRMEELLASRVDLLESEQT
                     AKDSAEAVSGQSVPEQADDDYRPVNNAFYRGEMHPEMIRGDVDPPGDLRSEVQPPGSG
                     STERLPAKRGSFGEEHSLGGDALGGGRSLPAVVEEFLTLDVHPPLMRESAVPGEQTIR
                     YPEWDYMLQDYRVNWCRVLERPADSGSDETVSATLTTHRSDIRSLRRFFESLRPPAFR
                     RLAGQADGEDLDLDAVVRRAAEQAAGFEGSDRVYVRREKKDRNVAVALLVDVSGSTGR
                     QLEGGRRVIDVERDSLVLLCEALEAVGDQYALYAYSGQGRGSVDVLAIKGFDERLGPA
                     TAQRLGGMSPRQQNRDGAAIRHAVAALMRREVKTRLLLLLSDGRPLDGDYKEEYALED
                     TKAALREANRQGIHPFCVTIDREADSYMRRMYGDVQYTVIDRVESLPARLPRIYQRFA
                     T"
     gene            2121..2525
                     /locus_tag="VFR82_12800"
     CDS             2121..2525
                     /locus_tag="VFR82_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502523.1"
                     /translation="MTDQPARRSVPPWLYKLFTGHQYPYVRRLAKFAQPSTPGEERPE
                     PTKDMIEAKFWEVYPHCSVKILQEVKRGMIVVFHDLGEYPPGAYQELVDNPEDFLAKT
                     YGKKKIKVNFYHGENFVCTINFKVAGWTEHEH"
     gene            2601..3542
                     /gene="mdh"
                     /locus_tag="VFR82_12805"
     CDS             2601..3542
                     /gene="mdh"
                     /locus_tag="VFR82_12805"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEU4502524.1"
                     /translation="MGRPKITVVGAGNVGGTTAQRLAEKDTYDVVLVDIVEGVPQGKA
                     LDISQAGPVCGYSTKVVGTNGYEESAGSSIAVITSGIPRKPGMSRDELLATNAKIVSS
                     VVTELVSRSPDIILILVTNPLDAMVHVARKVSGLPKSRIIGMAGILDSARMRTFIAQE
                     LSVPAPDVEAMVLGGHGDTMVPLPRYTTVKGKPISDLMPKNRLEAIVQRTRDGGAEIV
                     GLLKSGSAFYAPSASAVDMVEAIHKDQKRVMPCAVFCDGEYGLRNVVVGVPVKLGKGG
                     AEQIIVYDLTPVEQKALEASANAVRELCATVDRLMTN"
     gene            3616..4203
                     /locus_tag="VFR82_12810"
     CDS             3616..4203
                     /locus_tag="VFR82_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEU4502525.1"
                     /translation="MKFLEDPMQTMGAGFALALVLIVLYLGMTGIGAGEVDWTGIVIR
                     WIHFLAGITWIGLLYFFNLINAGFLKSLEGPTKNIVIPKLMPSALNWFRHGATVTVLA
                     GIILYIKLYMNGGTGAYALGIGGLLGIIMMVNVHAIIWPNQKKIIAAVTAAAQGTPAP
                     ADMAQWGRTALLASRVNFLLSIPMLFFMGAGSHFK"
     gene            4425..5723
                     /gene="nuoF"
                     /locus_tag="VFR82_12815"
     CDS             4425..5723
                     /gene="nuoF"
                     /locus_tag="VFR82_12815"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEU4502526.1"
                     /translation="MTLTEARLIEKTDGPPWDIDAYVKVGGYEAWKKCVQGLTPDQVV
                     QELKTSGLRGRGGAGFPTGVKWDKVLNHRVKERYFVCNAGEHEPGTFKDRYLLKTIPH
                     QLIEGCLIASRTAQAKASFIYVNHEYHEERENLKKALAQAKERGLVGKNILGSGIDID
                     LQVFEGHGSYVAGEETAMLESMQGRPAMPRQKPPFYPTDYGLYGKPTLVNNVETLCNI
                     PRILHKGAAWFTQVGTEKCPGTMMFSLSGAVNRPGVYEMPMGVTVRELIEQCGGGVPG
                     GRKIKAVFPGGPSFSMVTADQLDLPMDFDSLKKAGTGLGSAGVIVVDDATCMVRQTLK
                     FSNFFKGESCGQCPPCRMGTINLATLMTKIEAGEGTQKDLDSLLQLCGFVKGTGYCTL
                     VTGASVLVQSSVKLFRHEYEEHIRLQRCPYPSVPVEAVAH"
     gene            5734..6078
                     /locus_tag="VFR82_12820"
     CDS             5734..6078
                     /locus_tag="VFR82_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU4502527.1"
                     /translation="MPLVTFLHPKGKSGKVPVNTTLLDAAKELGFPLNHDCGGNASCT
                     TCRVEVQSGSDHLSEVDFEEQDLLDREALSEPWHRLGCQAKILGDVVVKVPETKWKAP
                     TTQSEAQGVDIR"
     gene            6135..6455
                     /gene="erpA"
                     /locus_tag="VFR82_12825"
     CDS             6135..6455
                     /gene="erpA"
                     /locus_tag="VFR82_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEU4502528.1"
                     /translation="MVTITQVAEQKIQELMKEEKDVVGLRIYVRGGGCHGYQYGMAFE
                     SKMAEDDTVIEKGDVKVIMDSQSAPLLQGAEVDYVDSVQGSGFSIKNPQAKTTCGCGS
                     SFSA"
     gene            6580..6993
                     /locus_tag="VFR82_12830"
     CDS             6580..6993
                     /locus_tag="VFR82_12830"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEU4502529.1"
                     /translation="MPRVSLTRRYRFCAAHRLHTDFLTPQQNWAAFGKCNNPNGHGHN
                     YVVLVTVKSEMGEETCQFERLDRIVEKTIVSRFDHQDLNQDPAFINTTTTGENLVKLI
                     WDLLVKEMPEGSLEKVGVIETRDNYFEYAAPAAVH"
     gene            7094..7654
                     /gene="folE"
                     /locus_tag="VFR82_12835"
     CDS             7094..7654
                     /gene="folE"
                     /locus_tag="VFR82_12835"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU4502530.1"
                     /translation="MTGLVSQLLRALGESPSRNGLLKTPERVAKALLFMTRGYHEDIE
                     RLLNGALFPIEYDEMVIVKDIDFFSMCEHHMLPFFGKCHVGYLPNKKVVGLSKIPRVV
                     DAFARRLQVQERLTTQIAETLKRKLDAHGVAVVMEARHLCMMMRGVEKQNTLAVTSSM
                     LGAFRTQEQTRNEFLKLIRRNAVGEA"
     gene            complement(7651..8454)
                     /locus_tag="VFR82_12840"
     CDS             complement(7651..8454)
                     /locus_tag="VFR82_12840"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU4502531.1"
                     /translation="MKTQVHGVTIAYDDQGAGFPIVFLHAFPLNRTMWSEQVAALSRR
                     FRAIAIDLRGHGESDAPFWRYSLEQYALDVKELLAGLGIRKALFVGLSMGGYLEFALY
                     RLSPDLMLGAVLADTRAEADKPEQIQWRYELAQRTAAIGPAAVIAEMLPKLLAPKTYE
                     RNPDLVARVKSMQAAAPVPGIIGDLIAIAERPDSTDLLSSMTIPALVIVGQEDVLTTP
                     ADAERIAKGIHGAQLIVIPNAGHLSNLEQPEHFTAAIEAFATVLQKTNP"
     gene            8566..>9700
                     /locus_tag="VFR82_12845"
     CDS             8566..>9700
                     /locus_tag="VFR82_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU4502532.1"
                     /translation="MKRPYEPAADLFDAPKHEGTANAPLAERMRPQSFDDLTGQDEIV
                     GKGKSLRSAIEADRLSSVIIWGPPGCGKTTLAYLISRSTKAHFVAFSAVTSGVPELRE
                     ILKGADHRLRTSGQKTILFVDEIHRFNKAQQDAFLPHVEKGTIILVGATTENPSFEVV
                     APLLSRSLVAVLRPLSDEALERILDRALADADRGLGGRRIQIAEDARRRLIGFANGDG
                     RSLLMALEFVAEQAVLQPDGSSRLDDATVAAALLKKTLRYDKSGEEHYNLISAYIKSL
                     RDSDPDGALYWLARMLEAGEDPKFIARRLVIFASEDIGNADPLGIVIAAAVAQAVHFV
                     GLPEAQINLAHGTTFLATRPKDNASYTALLEALSDARAEGNLSV"
BASE COUNT         2138 a         2597 c         2941 g         2024 t
ORIGIN      
        1 gtcctggaat atagtccgga aggcgcgcgg gcatattttg ccaccgagtc cctcaaggcg
       61 ctttcatcgg tggagcacgc gctgagcggc gttccgcttc gacaagtcgc tcgccagatc
      121 aagctggtcg tccaagggct ctgcggatcc gatgtatcca tcatcgcgcg gccggaggcg
      181 ttcaccacgc cgatcatccg cccgatcgtg agcgccgatg gtaagacgat tgcgatgccg
      241 gctctcctgc gccgatatcc cactgcccgg caaaacgaac ggctctatct cgtcatggct
      301 gcccatgagg ccggccattt agagttcggc acgtacacat tgccaatcag ttcgctcctg
      361 gatctgcttg agacggtctg cgggcgttac gggaggccgc gtcccgccgc cctggagtcg
      421 ctcgctgcac tcttccgtct ctatcctcat ccgcgtctca tgcaggatct ttggacgatc
      481 ttggaggatg ctcgcgtcga gtctttcctt cagcgggagt atccgggcct tcaaaaggat
      541 ctggcacaat tcgctgcgga tacgatcctc cctcgtgatc ctgctcacgg cttgatggtg
      601 agagagctga ttgccgattg tctgttgcgc ctctccgtcg gagagtcgat gtcctcggcg
      661 gttcctcacg ccgtcacgga agaggtctcc attctttgga acttatgcca acctgtattg
      721 acgcccgact cgacagcaga gcgtagcgtg cgcactgctc acgaagtgta cgtgaggatg
      781 gaagaactat tggcctcccg tgtcgacctg cttgagtcgg aacagacagc caaagattcg
      841 gcggaagctg tgagtggcca aagcgttccg gaacaggcag acgatgacta tcgtcccgtg
      901 aataacgcgt tttatcgggg ggagatgcac cctgaaatga tccgaggaga tgtcgatccg
      961 ccgggcgacc tccgctcaga ggtccagccg cccgggagcg gctcaacaga acggctacca
     1021 gcgaagagag gcagcttcgg agaagaacat tcactcggtg gtgacgcgct tggcggcggg
     1081 cgctctttac cggccgtcgt tgaggaattc ctgacgctcg atgtccatcc cccgttgatg
     1141 cgcgaatcgg ccgtgccagg cgagcagacg attcgctatc ccgagtggga ctacatgctt
     1201 caggactatc gtgtgaactg gtgtcgcgtg ctggagcgtc cggcggacag cggctcagat
     1261 gaaaccgtgt cggccacatt gactacgcat cggagtgaca tcaggtcgct cagacgattc
     1321 ttcgaaagtt tgcggccgcc ggcttttcgc cgtcttgccg gacaggccga cggtgaagac
     1381 ctggaccttg atgcggtcgt gagaagagcg gccgagcaag cagcgggctt cgaagggagc
     1441 gatcgggtct acgtcaggcg agaaaaaaaa gatcgcaatg tcgccgtcgc attgctggtt
     1501 gacgtgagtg gatcgaccgg acgccagctc gaaggcggac gccgcgtgat tgacgtagaa
     1561 agggacagtc tcgtcctgct ctgtgaggcg ttggaggcag tgggcgacca gtacgcactc
     1621 tatgcctatt ccggccaggg acgaggatcg gtggatgtgc tggcgatcaa gggttttgac
     1681 gagcggttgg gtcccgccac tgcgcaacgg ctgggtggca tgagtccccg gcagcagaac
     1741 cgcgacggcg cggcgatccg acatgcagtg gccgccctga tgcgacgaga ggtgaagacc
     1801 cgtctcctcc tgctcttgag cgatggtcgg ccgctcgacg gcgattataa ggaggagtac
     1861 gccctcgagg atacgaaggc cgcgttgcgt gaggccaatc ggcaaggcat ccatccattc
     1921 tgcgtcacca tcgaccgcga agccgacagc tatatgcgcc ggatgtacgg tgacgtgcaa
     1981 tacacggtca tcgaccgagt ggagtcactt ccggccagac ttccgcgaat ctaccagcgg
     2041 ttcgctacct agcggccaaa ggtaaatgga acgagtgtgc aatgttggat gttgatgaca
     2101 ggaataaccg tggcggaagc atgacagatc aacctgcccg taggtccgtg cctccctggt
     2161 tgtataagct ctttaccggc catcagtatc cctatgtgcg ccgattggcc aagtttgctc
     2221 aaccctccac gccgggcgaa gagcgaccgg aaccgacaaa agacatgatc gaagccaagt
     2281 tttgggaggt gtaccctcat tgctccgtca agatcctgca ggaagtgaaa aggggaatga
     2341 tcgtggtatt tcacgatctc ggcgagtacc cgccgggggc gtatcaagaa ctcgtagaca
     2401 accctgaaga ctttctggca aaaacgtatg gcaaaaagaa aatcaaggtg aatttctatc
     2461 acggcgaaaa tttcgtgtgc acgatcaact tcaaagtggc aggctggacg gaacacgaac
     2521 actaggagcg aacgttcatg gccggcttcg ggcgccggcc gtgagcatcc agggtcttta
     2581 gagtggtggc cggagggcgt atggggcgac cgaaaatcac cgtcgtggga gcaggcaatg
     2641 tcggtggaac aacggcgcaa cggctggcgg aaaaagatac ctatgacgtc gtactggtgg
     2701 atatcgtgga aggcgtgcct caaggcaaag cgctcgatat ctcacaggcg ggtcccgtct
     2761 gcggctatag cacgaaagtc gtcggaacca acgggtacga ggaatcggcc ggatcctcca
     2821 ttgctgtgat cacctcggga attccccgca agcccgggat gagccgggat gaactgctcg
     2881 cgaccaacgc aaagatcgtg agctcggttg tgacagagtt ggtctcgcgt tctcctgaca
     2941 tcattctgat cctggtgacc aatccgttgg atgccatggt ccatgtggct cgaaaagtca
     3001 gcgggttgcc gaagtcgcgt atcatcggca tggccggcat cctcgactct gcccgcatgc
     3061 gcacctttat cgctcaagaa ctcagcgttc ctgctccgga cgttgaagcc atggtcttgg
     3121 gtgggcacgg cgataccatg gttccactac cgcgttatac gaccgtcaag ggtaagccga
     3181 tctcagatct catgccgaaa aaccgtctcg aggccattgt gcaacgtacg cgcgacggag
     3241 gcgcggagat tgtcggattg ctcaagtccg gaagtgcgtt ttatgcgccc tccgcatcgg
     3301 ccgtggacat ggtcgaggca attcacaagg atcagaaacg agtgatgcct tgtgccgtct
     3361 tctgcgacgg cgaatacggg ctgcggaatg tcgtcgtcgg cgtgcccgtg aagctcggaa
     3421 aagggggtgc agagcagatc atagtctatg atctgactcc ggttgagcag aaggcgctcg
     3481 aagcttcggc gaatgccgtg cgcgaactgt gcgcgacggt tgaccgcctg atgacgaact
     3541 gaatgccatc gcttgacaag caggaagggc tcaagtacag aagcggctta gcagtgcacc
     3601 gaatgaggag atgttatgaa atttcttgaa gatccgatgc agaccatggg cgcaggtttt
     3661 gcgctggcgc tcgtcctgat tgtcttatac ctcggtatga caggcatagg agccggagag
     3721 gttgattgga ccggaatcgt cattcgctgg atccatttcc tggccggtat tacctggatc
     3781 ggtctgttgt atttcttcaa cctgatcaat gccgggtttc tgaaaagcct agaaggtccc
     3841 acgaagaata tcgtcatccc caaattgatg ccatccgcat tgaattggtt ccggcatggt
     3901 gcaacagtga ccgtgctggc ggggatcatt ctctacatca aactgtatat gaacggggga
     3961 accggtgcct atgcgctggg aatcggtggg ctgctcggta tcattatgat ggtgaacgtc
     4021 catgcgatta tatggccgaa tcaaaagaag atcattgctg cggtcacagc ggcagcgcaa
     4081 ggtacgcccg ccccggcaga catggcgcaa tggggcagga ccgccttact ggcgtctcgg
     4141 gtgaatttcc tgctttcgat ccccatgctc tttttcatgg gtgcggggag ccacttcaaa
     4201 taagcccgtc tgcaccggct ggtgtaagca cctgcaacca gccggttttc ctctcgatca
     4261 gtcagggcct cacatatcgg ccattatacc cactgcggag tagggctctc gtcctaacct
     4321 gttgagattg aggcctattc tgcccacttc tccttgacgg agggccgaag cggcccttat
     4381 agtacccact caccgttttc ggctctaggg gcagaaggat cttgatgact ctgacggaag
     4441 cacgcctcat cgagaagacc gatgggcctc catgggacat tgacgcctat gtcaaagtcg
     4501 gaggctatga ggcctggaaa aagtgcgtcc agggactgac gccagatcaa gtggtccaag
     4561 aactcaaaac gtcggggcta cgcggccggg gtggtgccgg ttttccgacc ggcgtcaagt
     4621 gggataaggt tctcaatcat cgggtcaaag aacgatattt tgtctgcaat gccggtgagc
     4681 acgagcccgg tacctttaag gacaggtact tgctgaaaac aattccccat caattgattg
     4741 aaggctgtct cattgcctct cggacggcac aggcgaaagc ctcattcatt tacgtcaatc
     4801 acgaatatca cgaagagcgg gaaaatctga aaaaggcatt ggctcaggcc aaggaacggg
     4861 gcttagtcgg caagaatata ctcggtagcg gcatcgacat cgacctacag gtattcgagg
     4921 gccacggaag ctacgtggcc ggcgaagaaa cggccatgtt ggagtccatg caaggacgcc
     4981 ctgccatgcc gaggcaaaaa ccgccgtttt atcccaccga ttacgggttg tacggcaaac
     5041 cgacgctggt caacaatgtg gagaccttgt gcaatatccc ccgcattctc cacaagggtg
     5101 cggcgtggtt tacgcaagta gggacggaga agtgtccggg cacgatgatg ttttcattga
     5161 gcggcgccgt gaaccgtccc ggcgtctatg aaatgccgat gggcgtcacg gtgcgtgaat
     5221 tgattgagca gtgcggcggt ggtgtaccgg gtggacgaaa gatcaaggcc gtgtttccgg
     5281 ggggaccgtc gttctcgatg gtcacggccg atcaactcga tctgccgatg gattttgatt
     5341 cgttaaagaa ggctgggacg ggattaggtt cggccggggt gattgtcgtc gacgatgcca
     5401 cctgcatggt cagacagacg ttgaagtttt cgaacttttt caagggcgaa agctgcggac
     5461 agtgtccgcc gtgccggatg ggcacgatta atctggccac tctgatgacc aagatcgaag
     5521 caggcgaggg cactcaaaag gatctggaca gcttgttaca gctctgcggc ttcgtcaaag
     5581 gaacgggata ctgtacgctg gtgacgggag catcggtgct cgtgcagagc agcgtgaagc
     5641 tgttccgtca cgagtatgag gaacatattc gattgcaacg ctgtccgtat ccgtcggttc
     5701 ctgttgaggc tgttgcccac tgaaggaggg tggatgccgc tggttacatt tctgcaccca
     5761 aaaggaaaaa gcgggaaagt tcctgtcaac acgacgctgc tggatgcggc aaaagagctc
     5821 gggtttcccc tgaatcatga ttgcgggggc aacgcctcct gcacgacgtg ccgggtagag
     5881 gtccaaagcg gcagtgacca cctctctgag gtcgactttg aagaacagga tctgctggat
     5941 cgggaagcgc ttagcgagcc ttggcaccgg ctagggtgcc aggcgaagat ccttggtgac
     6001 gtggtggtga aggttccgga aaccaaatgg aaagcgccga cgacccaatc ggaggcacag
     6061 ggtgttgata ttcggtaaag aggtgttaca ctaagccaac caggcggtct tcgcctgtga
     6121 gaaggaggag cacgatggtc acgattaccc aggttgccga acagaagatt caggaattga
     6181 tgaaagaaga aaaagacgtt gtcgggttgc ggatttacgt ccgcggcggc ggttgtcacg
     6241 ggtatcaata cggcatggca ttcgaatcca aaatggccga ggacgatact gtcattgaaa
     6301 aaggcgacgt gaaggtcatc atggactctc agagcgcacc gctccttcag ggagccgagg
     6361 tcgactatgt cgacagcgtg cagggatcgg gattctcgat caagaatccc caggccaaga
     6421 ccacctgcgg atgcggtagc tcattcagcg cataaaagcg gcgtgacctc ggctcgccgg
     6481 gggctagatg acagaggcca gggttgctcg ataagtagcc ctggcttttg tgttttcgac
     6541 ggttgagcct cagttggcac ccgattcagg acaaaccaca tgccacgcgt aagcctgacc
     6601 agacgctatc gcttctgtgc tgcccataga ctgcacactg attttttgac gcctcagcag
     6661 aactgggcgg cgttcggaaa gtgcaacaac cccaacgggc acggccacaa ctacgtcgtg
     6721 ttggtcacgg tcaagagtga gatgggagaa gagacctgcc aattcgagcg cctcgatcga
     6781 attgttgaga agacgattgt ctcgcgcttt gatcatcagg acttgaatca agaccctgcc
     6841 tttatcaaca cgaccacgac gggggaaaat ctggtgaaat tgatttggga cctcttagtc
     6901 aaagagatgc ccgaaggctc gcttgaaaaa gtgggcgtga tcgaaactcg agataattat
     6961 ttcgaatacg cggcgcctgc cgctgtccat taaccggagc agaagacagc ctatgccacg
     7021 ccgccgccgt agcgcttcga tcgcacatct ggaagatctt cccgccgtta ccgctcctca
     7081 agacgtcacg agaatgaccg gtctggtcag ccaactcttg cgtgcgctgg gcgagtctcc
     7141 gagccggaac gggctcctca agacaccgga gcgggtagcc aaggcgttgc tgttcatgac
     7201 ccgaggttat cacgaggaca tcgagcgatt gctcaatggt gccttgtttc ccatcgagta
     7261 cgacgagatg gtcatcgtca aggatatcga ttttttcagc atgtgcgagc accacatgct
     7321 tccattcttc ggaaagtgtc acgtcggcta tctgccgaac aaaaaggtcg tcggactcag
     7381 caagatcccc cgtgtcgttg atgccttcgc tcgacggctg caggtgcaag agcggctcac
     7441 gacgcaaatc gccgagacgc ttaagcgaaa gttggatgcc cacggcgtcg ccgttgtcat
     7501 ggaagcccgc cacttgtgca tgatgatgag aggggttgaa aaacagaata ccctcgcggt
     7561 cacaagctcc atgctggggg cgtttcggac acaggaacaa accagaaacg aattcttgaa
     7621 actgattaga aggaacgccg tcggcgaagc ttaagggttt gtcttctgaa ggactgtcgc
     7681 aaacgcttcg atggccgcgg tgaaatgctc gggttgctcg aggttactga ggtggccagc
     7741 gttcggaatc acgatgagct gggcgccatg gatgcctttg gcgatccgtt cggcgtctgc
     7801 cggcgtggtt aagacatcct cttggccgac gatcaccagc gctggaatcg tcatggacga
     7861 taggagatct gtggagtcgg gcctctccgc tatcgcgatg aggtcgccga taattcccgg
     7921 cacgggcgcc gctgcctgca tggacttgac gcgagccacg agatcagggt tccgctcgta
     7981 ggtcttcggc gccagcagct ttggcagcat ttcggcaatg actgccgcag gcccaatggc
     8041 tgcggtccgt tgagcaagtt cgtagcgcca ttgaatctgt tccggtttgt cggcctcggc
     8101 gcgcgtgtct gccaggacgg ctccgagcat gaggtcggga gacagacggt acagggcgaa
     8161 ttcgaggtat ccacccatcg agagtcccac gaacagagct ttccggatac ccagccccgc
     8221 tagcaactcc ttcacatcca aggcatattg ttcgagactg taccgccaga acggagcgtc
     8281 tgattcaccg tgtccgcgaa ggtcgatcgc gattgctcga aaccgccggc tgagcgctgc
     8341 cacttgctca gaccacatgg tgcggttgag tgggaaggca tggagaaaga cgatcgggaa
     8401 acccgctccc tgatcgtcat aggcgatggt tacaccgtgc acctgagtct tcattgaact
     8461 cattggtgtg tcgtaacacc gccttccaga cgggtcaaga ctgtcgaagg tgaatttgct
     8521 ctcaatcgga agcggcgtat agaattttga tcgtcagagg gttgcatgaa acgtccatat
     8581 gaaccggccg ccgatttgtt tgacgctccg aagcacgaag gcactgcgaa tgcgcctctg
     8641 gctgaacgga tgcgtcccca atcgtttgac gatctcacgg gacaggatga gatcgttgga
     8701 aaggggaaat cgctccgcag cgcgattgaa gccgatcgat tgtcctccgt gatcatctgg
     8761 gggccgcccg gctgcggcaa aaccacgttg gcctatctga tctcacgatc aacgaaagcc
     8821 cattttgtcg ccttctctgc tgtcaccagc ggagttcctg aattgcggga aatcctgaaa
     8881 ggagccgacc atcggctcag gaccagcggg cagaaaacca ttcttttcgt cgacgagatc
     8941 catcgtttca acaaagctca gcaggatgcc ttcctccccc atgtggaaaa aggcacgatt
     9001 atcctcgtgg gagcaaccac agaaaacccc tccttcgaag tcgtggcgcc gttgctttcg
     9061 cgttcgttgg tggcggtgct gcggcctctg agcgacgaag ccttggagcg catactcgat
     9121 cgtgccctcg cggacgctga ccggggactg ggcggccggc gaattcagat agccgaagac
     9181 gcgcgtcgac gcctcatcgg ctttgcgaac ggcgatgggc gttcattact catggccttg
     9241 gagtttgtgg cagagcaagc cgtcctgcaa ccggacggca gcagccggct tgacgatgcc
     9301 acggtagcag ctgccctact taaaaaaacg cttcgctatg ataagtccgg agaagagcac
     9361 tacaacctga tctcggccta tatcaagagt ctgcgcgatt ccgatcccga cggggccttg
     9421 tactggctgg cgagaatgct ggaagcaggc gaggatccga agttcatcgc gaggcgtctg
     9481 gtcatttttg cgtcggaaga cattggaaat gccgatccgc ttggcattgt catcgcggcg
     9541 gctgtggcac aggccgttca tttcgtcggg ttgcccgagg cgcagatcaa cctggcgcat
     9601 ggcacaacct tcttggcgac cagaccaaag gacaatgcat cctacacggc acttctcgag
     9661 gcgctgtcgg atgcgagggc agagggaaat ctgagcgttc
//
LOCUS       DASTQY010000394         3521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1295407, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000394 DASTQY010000000
VERSION     DASTQY010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295407"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VFR82_12850"
     CDS             complement(<1..1135)
                     /locus_tag="VFR82_12850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU4502533.1"
                     /translation="MANYTSYDELLQENAALKERLAEQERLGEQSRQAEEQGRIAEHF
                     RRMAAETALRESEARYRALADTMPQVVFIATASGFVEYVNRPGLEYIGLRLDEAIGFG
                     WLGAIHPEDRQLVSERWLECLRIGKTYEFENRLLRADGHYRWQLARAVPIVGESGAIV
                     RWIGTSTDIHDHRQAQQDLQESRTEAECQAEELNRLYQTSPAGLAFLDTELRFVRINQ
                     TLAQINGFPPEAYIGRSLREMLSPAMANRLEPVLRLVVDEAKPAMNVEFEGEPPSDPG
                     SIRYWLANYYPLVNREGAVLGVNVVMQDMTERKRTEEALERSHAFVRQIIDTDPNFIF
                     VKDRDGRFTLVNQAVADCYGTTVEELLGKTDADFNSNVEEVEQF"
     gene            complement(1183..>3521)
                     /locus_tag="VFR82_12855"
     CDS             complement(1183..>3521)
                     /locus_tag="VFR82_12855"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502534.1"
                     /translation="EDRAEHESQVGTLLLGGISEVSLEQRLMRPDGETIWVTVTLSIQ
                     WDAGGRIQSLIGVIEDIDARKRAELELRTHTARQQLLSKAMDQLLRVDDPRDVMQDIF
                     ESLRKSVGLDTFLDYDVMPDNSGIRMATWAGIPDLALEPLMFLQFGQALCGTSAVSRQ
                     PVIVDHLQSSSDPKASRMQAVGSTAYACFPLIVGDRLLGTLAFASSTRNSFEPDDLEL
                     LEAVSNYVAMAKERRRLLHETRQRAEQVAEQEERLRFATEGAGMGTWDVDLRTGRVVW
                     NRQNALLLGYDFDSDQASTKRWWALVHEEDRAIVRQAMKNARNSGHLYQAEHRIRRAD
                     TGELRWLAPYGRIHYNGGGRPTRFVGVTFDITERKQSDIQRQEWTRELERRVNERTKE
                     LVRTHDRLRGLAQDLTLTEQRERQRIAGELHDYLAQMLVVTRLKMNQATQMISPHPAC
                     SVLQEADQLIDQSLTYTRTVVAELMPPTLRQFGLAAGLRWLGERMGQQGLDVNVDVPD
                     VAPPLSEDHTTMLYQCVRELLFNVLKHAGIDRASVKMGDDGQGKLHIVVHDDGVGCDP
                     VTIRESKGDKFGLFSIRERMEAIDGWMELDSQPGGGTTVTLGVTIGASGMEDATVDER
                     PLTAAQPSGTSGFNVHHPAHERQAPVRVLLVDDHAMVRQGLRSILDGYANLHIIAEAG
                     DGEEAITLARLLKPDLIVMDVNMPRLDGIEATRRIREELPGTYVVGLSVNEAEHVVKS
                     MRDAGASAFLTKESAADHLYETIGMVTRKTSVECSMDA"
BASE COUNT          708 a         1086 c          954 g          773 t
ORIGIN      
        1 ggaactgctc gacttcttcg acattcgaat taaaatccgc atcggttttc cctaacagtt
       61 cttccactgt agtaccgtag caatcggcaa cggcttggtt caccagcgta aagcgaccgt
      121 cccgatcttt gacaaagata aaattgggat ccgtatcgat gatctggcgg acgaaggcat
      181 gcgatcgctc gagagcttct tcagtccgtt tgcgttccgt catgtcctgc atgacgacat
      241 tgactccaag caccgctccc tcgcgattga caagcgggta atagttcgca agccaatagc
      301 ggatactgcc aggatctgag ggcggctctc cttcgaactc cacgttcatc gccggctttg
      361 cctcgtcgac tacgagccga agaaccggct cgagccgatt tgccatagca ggagacagca
      421 tctcgcgaag gctccggccg atatacgcct caggaggaaa gccgttgatc tgcgccaatg
      481 tctggttgat gcggacgaag cgcaattcgg tgtcaaggaa cgccaagccg gcaggactcg
      541 tctgataaag ccgattcagc tcctcggcct gacattcggc ttcagtacgg ctttcctgca
      601 agtcctgttg ggcttgtcga tggtcgtgaa tgtcggtaga ggtgccgatc caacggacga
      661 tggctccgct ttctccgaca atggggacag cgcgggctaa ctgccagcga taatgaccgt
      721 ccgcgcgcaa gaggcgattt tcgaactcat aggtcttccc gattctcagg cattcgagcc
      781 atcgctcaga aaccaattgc cggtcctcgg ggtgaatcgc tcccaaccac ccaaagccga
      841 tcgcttcgtc aagcctgagg ccgatgtact caagccccgg gcgattgacg tattcgacga
      901 atccagaggc cgtggcgata aagacgactt gcggcatggt atcggccagc gcccgatacc
      961 gcgcctcgct ttcgcgcagg gctgtttccg ccgccatacg ccggaagtgt tcggcaatgc
     1021 ggccttgttc ttccgcctgc cgactctgtt cccccaagcg ttcctgctcg gcgagtcgct
     1081 ccttgagcgc ggcattttct tggagcagtt cgtcgtatga tgtgtagttg gccatcgaaa
     1141 gaaccatgcc aatgggattt caatgcatgc gtggactgat ttttatgcat ccattgaaca
     1201 ttcgacgctg gtctttcgtg tcaccatccc gatcgtttcg tataagtgat cggccgcact
     1261 ttctttggtc agaaacgcgg aagcgccggc gtccctcatg gatttcacga cgtgctccgc
     1321 ctcgttcacc gatagaccga ccacatacgt cccgggtaat tcttctcgaa tccgtctcgt
     1381 cgcctcgatg ccgtccaacc gtggcatgtt gacgtccatc acgatcaagt cgggtttgag
     1441 caatctcgcc aacgtgatgg cttcttctcc gtcccctgcc tcggcaataa tgtgcagatt
     1501 ggcgtaccca tcgagaatgc tacgtaaacc ctgccgcacc attgcgtgat cgtcgacgag
     1561 gagtacccgt acgggggctt ggcgctcgtg cgcgggatga tgcacattga atcctgacgt
     1621 gccggacggt tgcgccgcgg taagcggccg ctcatctacg gtcgcgtcct ccatcccgct
     1681 cgcgccaatt gtgacgccaa gggtaacggt cgtaccgcct cccggttgtg aatccaactc
     1741 catccatccg tcgatcgctt ccatccgctc gcgaatgctg aaaagcccga acttgtctcc
     1801 cttagactcg cgaatggtga ctggatcgca gcccaccccg tcgtcatgca ccacaatgtg
     1861 caattttccc tggccgtcat cgcccatctt cactgacgcg cgatcgatgc cggcgtgctt
     1921 taagacgttg aacaataatt cgcgaacaca ttgatacaac atggtcgtat ggtcttcgga
     1981 caagggagga gccacgtccg gcacgtccac attgacgtcc aagccttgct ggcccatgcg
     2041 ttcaccgagc cagcgcaagc cagctgccaa gccgaactgg cgcaaggtcg gtggcatcag
     2101 ctccgccacg accgtgcgtg tgtaggtaag gctttgatcg atcagttggt ctgcttcctg
     2161 gaggacggaa caggcgggat gcggcgagat catttgagtc gcctgattca tcttcagtcg
     2221 tgtgaccacc aacatctgcg cgagataatc atgcagttcg ccggcgatgc gttgacgctc
     2281 tcgctgctcc gtcagcgtta aatcctgtgc gagaccgcgg agccgatcat gcgtccgcac
     2341 gagctctttt gtgcgttcat tgacgcgtcg ttccaactct ctcgtccatt cctgccgctg
     2401 gatgtcggac tgcttgcgct ccgtgatgtc gaatgtcact ccgacgaagc gggtcggacg
     2461 cccgccgccg ttgtaatgga tacgaccgta cggagcgagc cacctgagct cgccggtgtc
     2521 cgcacgacgg atccggtgtt ccgcctgata gagatggccg ctgttcctgg cgttcttcat
     2581 cgcctgccgc acaatcgccc ggtcttcttc atgcacgagg gcccaccaac gcttcgtcga
     2641 ggcttgatcg gaatcaaaat cgtatccaag caggagggca ttctgccgat tccacacaac
     2701 ccggccagtc cgcaagtcca cgtcccaggt acccatcccc gccccttcgg ttgcaaagcg
     2761 aaggcgctcc tcctgctcag cgacctgctc ggcccgttgc cgtgtttcat gaagtagacg
     2821 acgtcgctcc ttcgccatcg cgacataatt ggaaacggcc tcgagtaact ccaggtcatc
     2881 gggttcgaag gagttgcgcg tgctgctggc aaatgcgagc gtccccagta accggtcacc
     2941 gacgatcagc gggaaacagg cgtaggcggt ggagccgacc gcttgcatgc gagaggcttt
     3001 cggatcagac gaggattgca ggtgatcaac gatgaccggc tgccgtgaaa ctgccgaggt
     3061 cccacaaagc gcttggccga actgcagaaa cataagcggt tcaagggcca gatcaggaat
     3121 gcccgcccac gtcgccatac ggataccgct gttgtcaggc atgacgtcat agtcgagaaa
     3181 cgtgtccagg cccacgcttt tacgcaagga ttcgaagata tcctgcatga cgtcgcgcgg
     3241 atcatcgacc cgcagcaatt gatccattgc cttgctcagc aactgctgac gcgcggtatg
     3301 agtacgcagc tccagttccg ccctcttgcg ggcatcgatg tcttcaataa cgccgattaa
     3361 cgattggatt cgaccaccgg cgtcccactg aatcgacaaa gtgaccgtca cccaaatcgt
     3421 ctctccgtcc ggccgcatga gacgctgctc caaggacacc tcggaaatcc cacccagtag
     3481 gagcgtcccc acttgactct catgttcggc gcggtcttcc g
//
LOCUS       DASTQY010000395         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1295546, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000395 DASTQY010000000
VERSION     DASTQY010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295546"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1233
                     /locus_tag="VFR82_12860"
     CDS             82..1233
                     /locus_tag="VFR82_12860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502535.1"
                     /translation="MPSTIFVIDSSPAVRRMVEQISTPEGYDVVGFQDGPAALEAARR
                     MSPALIITDYHLDSMTFSGFCKEVHKLDNLADTYIIALTGATDKLENTHLKALGVKAF
                     LKKPFQSEHLLDLIKDLDRTNTTNISGKKKRSWPPTSSALDSDDECAGDSADAMSDEE
                     LHPIIPMTLDVTPVKKEAMKPPVPESASTPPAAEPEEAMKGLFGQLLESMSGKTEKKI
                     ADMLPHMVNTGLEAQVSRAVEAEVRLQLGAAVSQERLTEIIEPLVMQALPKILTREMA
                     VLEPIIRHSMVEIASPLIKEHIEHMVREQAETVRQSLQDAVREHLRSVEEEVRAQLKE
                     AATAETKKLATDVVRTIAEDQIRTSVQMVAPSIVEEQIRAEIARLTEAA"
     gene            1518..>2368
                     /locus_tag="VFR82_12865"
     CDS             1518..>2368
                     /locus_tag="VFR82_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU4502536.1"
                     /translation="MAPLQLDKTYDPKAVESRWYQFWAERQYFHADPSHPGRPYSIVI
                     PPPNVTGSLHVGHALNHSLQDILIRWRRMQGRNTLWLPGTDHAGIATQNVVEKQLLGE
                     GISREALGRDGFVERVWQWKAASGNRIIEQQKQLGESCDWERLRFTMDAGLSKAVLEV
                     FVRLYEDGLIYRGERLINWCPRCLTALSDIEVEHEDVKGTLYHIRYPLTDDPSTSLTV
                     ATTRPETMLGDTAVAVHPDDPRYRHLIGKSIRLPLTQRTIPVVGDAILVDLEFGTGAV
                     KITPAHD"
BASE COUNT          545 a          709 c          648 g          466 t
ORIGIN      
        1 tggcgcatct attagccgtt ttcttgacaa agaatacccc ccctgctagc ttcgaactag
       61 ccaataaaat tggagactta agtgccttca acgatcttcg tcatcgacag cagcccggcc
      121 gtccgtcgta tggttgaaca gatttcaacc cccgaagggt atgacgtggt ggggtttcag
      181 gacgggccgg cggcgctgga agccgcccgc aggatgagtc ccgcgctgat catcaccgac
      241 taccacctcg acagcatgac cttttcgggg ttctgtaaag aagtgcacaa attagacaat
      301 ctcgctgata cctatatcat cgcgctcacg ggtgcgacgg acaagctgga aaacacgcac
      361 ctgaaggctc ttggcgtcaa agccttcctc aagaaaccgt ttcagtcgga acatttgttg
      421 gacctcatca aagacctgga ccgtacgaac acgacgaaca tcagcggaaa gaagaaacgg
      481 tcctggcccc cgacatcgag cgcgcttgat tccgatgatg agtgtgcagg cgattcggcc
      541 gacgccatga gcgacgagga gttgcatccg atcattccga tgaccctcga cgtgacaccc
      601 gttaagaaag aggccatgaa accgcccgtg cccgaatccg cctcgactcc ccctgcagcg
      661 gaaccggaag aagcaatgaa aggcctcttc ggccagttgt tggaatcgat gtccggaaaa
      721 acagagaaga aaattgccga catgctgccc cacatggtga acacagggct tgaagctcag
      781 gtgtccagag ccgtcgaggc agaagtgcgt ctgcaactgg gcgccgccgt gtcgcaggag
      841 agactgaccg agataatcga accgctggtc atgcaggcgc tcccgaaaat cttgacgagg
      901 gaaatggccg tactggaacc gatcatccga cacagcatgg tcgagatcgc cagcccgttg
      961 attaaggagc acatcgagca catggtgcgg gaacaggccg agacggtcag gcaatcactt
     1021 caggatgccg tacgcgaaca cttgagatcg gtggaagagg aggttcgggc gcaactcaaa
     1081 gaggctgcga cggctgaaac gaagaagctt gccactgacg tggtccggac catcgcagaa
     1141 gatcagatca gaacaagcgt ccagatggta gccccctcaa tcgtcgaaga acagattcgt
     1201 gccgaaatcg ctcggctgac cgaggccgcc tgacagcgaa atcttctagc cttttttccc
     1261 ctttttcgtg ccgcgtcgcc ctttcgccaa cgttttcatc cgttggacgt tcttccgatg
     1321 cttctttctg gtcttgcggt ccttctctcg tcccgtggcc ataatctctc ctcggtcaag
     1381 cgtgaatgag cccgtcgact gcccggcaat tcggcgatga tatcacacaa gcctccctgc
     1441 ctctcaagga gaagaagtaa ccttgagggg tcccggcgcg catgttaaga tgcctcgcct
     1501 tctctccagc gcaaaccatg gcccctctcc aactcgacaa aacgtacgat ccgaaagccg
     1561 tcgaatcgcg atggtatcag ttctgggctg aacgccaata ctttcacgcc gatccctccc
     1621 atcctggccg gccctacagc atcgtcattc cgcctccgaa cgtcacaggc tcgttgcatg
     1681 tcggccacgc cttgaatcat tcgctgcaag atatcctgat acgttggcgc cgcatgcagg
     1741 gtcgcaacac actctggctt cccgggaccg accatgccgg catcgcgacg caaaacgtcg
     1801 tcgaaaagca gttgttgggc gaagggatct cccgtgaagc gctggggcgg gatggtttcg
     1861 tcgagcgggt ctggcagtgg aaggcggcgt cggggaaccg catcatcgag cagcaaaagc
     1921 agctcggaga atcctgtgac tgggaacgac tgcgcttcac gatggacgcg ggattgtcca
     1981 aggccgtgct ggaagtgttc gtccggctct acgaagacgg ccttatctat cgtggcgaac
     2041 gactaattaa ctggtgtccc cgctgcctga ccgccttatc cgacattgaa gtggaacacg
     2101 aggacgtgaa gggcacgctc tatcacattc gctatccgtt gaccgacgac ccaagcacct
     2161 cgttgaccgt cgccaccaca cgaccggaaa ccatgctcgg cgacacggcg gtggccgtgc
     2221 atcccgacga cccacgctat cgccatctca taggcaagtc gatccgattg ccgctcactc
     2281 aacggaccat tcccgttgtg ggcgatgcca tcctagtcga tctcgaattc ggcacgggtg
     2341 ccgtcaagat cacgccggcc catgattt
//
LOCUS       DASTQY010000396         9880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_81657, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000396 DASTQY010000000
VERSION     DASTQY010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9880
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81657"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            620..943
                     /locus_tag="VFR82_12870"
     CDS             620..943
                     /locus_tag="VFR82_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502537.1"
                     /translation="MGRLLSLVVTGAFSVSLLMVGAAQAVCDPLCSPNSNKSGAVRGL
                     DRADAVAGGGADGRTNAREKQGAVTLPPDADKDGVPDAQDQCLNVAGPGPSGCPVVEL
                     PPPGA"
     gene            1259..1747
                     /locus_tag="VFR82_12875"
     CDS             1259..1747
                     /locus_tag="VFR82_12875"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEU4502538.1"
                     /translation="MSALDRNSGGFTLIELMVVVGILTIAVALAVPEFVRWHVQSQLR
                     QATSEIATQLMLARMAAMNRNRSVDVTVQTTGAAVHISAVTPSSGAAVIKDKVFPSRV
                     TSVLGSPVTVSFSSLGMRTTGGTGTQTIGVCDTYGRQYSVAIIPSGKVNWSINSSGTP
                     CP"
     gene            1767..2261
                     /locus_tag="VFR82_12880"
     CDS             1767..2261
                     /locus_tag="VFR82_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502539.1"
                     /translation="MRLNRLKSQQGFSLLEGMLAAVLLGTGLLAMSAMQGIAIVKNVD
                     SNELTRITALASDMMERIQFNRRNAISYNGINTQSATNCNTISAATQPQARGDCLLWD
                     SLVDATQYQNVMGTVTVSDIIAPTALAQRNVAVTISWVGSLKSDTTVKRARSLTVNRV
                     VAPE"
     gene            2279..3331
                     /locus_tag="VFR82_12885"
     CDS             2279..3331
                     /locus_tag="VFR82_12885"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEU4502540.1"
                     /translation="MRRSTEQAGFTLIELMVGMAVSVIVVAAAFTILTTTSKTLRANE
                     QVVDTQQNLRMAMEMLARDFKLAGFGSPPAPIGNCTNGIVPADQNTGGADTGPDSVQL
                     LVPATRSSGANRWTLRLPTTAGTTVITLWPGAVTDMVNSGLTANASYISIGGAATAQV
                     TGFNATASTINVAIPPPLWFQTEDRVYLLQCIRYRVVPAPDPGNVCGGVAPCLTRGVA
                     GVVAGPNAEAPIAEGIEDLQLAYACDGCVTAINTGIPDRIIDDQNASNTFDQADFLSN
                     LSWTAAPLTPDKIQLVQVALVARQSKTDQGFSEANNTMVGSSTLTVTGDHSLPADANH
                     RRRILVKTVETRNVGM"
     gene            3396..3986
                     /locus_tag="VFR82_12890"
     CDS             3396..3986
                     /locus_tag="VFR82_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502541.1"
                     /translation="MAMLIVMMVLLMLTVLGIAAITVSGLGNTMAGLQRTMEASANAA
                     ESCLGTGVNIIQYVLLTENGRQIPSALLNSTPGGPVPSGNKTVLESEIAGNSQNNTDV
                     PIGPGGTPGAPNLQMSVGPYEVLGDIDFLYVKPRAGTGQMQFSGYEGAGSGGGNDVDA
                     FFRLTCIANNVATGTESQVSAIYACSMGGDGCQKQP"
     gene            4092..8465
                     /locus_tag="VFR82_12895"
     CDS             4092..8465
                     /locus_tag="VFR82_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502542.1"
                     /translation="MKRESFRTRWNRVLISISLFLSLGSPIAASAQNMADYTNYPVFL
                     NQTVPPNILFIVDLGNETLPAGYAGSGHKYPISFKASTPTAGSYASNVTFDATGTNAD
                     MVAVSDPGGTAMSTATTAAPADIFNSSKNYYGIFDPFRCYATGSNDFVYGSKKTTLSQ
                     ACGTSHWDGNFLNWLIGRKKDLTYQVLMGGTTNPASSNTDGTADFISSEKNTGEGGTT
                     ESCANDTRSCWRFVKYVPTATLAGRVPTGLPNPTVTGINVGTGTAADAGLWFGAGDGK
                     LYVNDNADVDPFNTANSNKYTLRVDLTTEPNIPSGTGNLSGSCDEGDTANFAGHMICY
                     RKDRSLGLFQKMRTDNMHVGVMFANALSGKGGKVMFNFDESFNSSAITNLRNEHIQPK
                     SPLSEALYEGLCLFRKSQGPCYNNSGASATGYTSSTGVASDPFYFTSFGQSLRCCKSF
                     VLMISPGQGVDDGNAPDLQTPFGNLFTGTNIGVVTTGAAGDRLDDVAFYGQTHDIREQ
                     ASPVGLAGTQSVTFYAVNAMGGQVGSTLLASASKYGGFEDRNSNGTADLTGQSCTYPA
                     GSSLGTGTSTSNAEWDVDQNCVPDTFFDASEGGDLEAQINLAISSILKKSASGTSISV
                     LASSSTGEGSIYQAFFYPSTFEGTKEIKWTGFVQGLFVDTYGNLREDRGGSGGSPDGR
                     LVYTDDNIVQTRLDAATGDVKVDRFVDADGDGKADSTTPYESVGLREMQGIWEAGKKL
                     ALRDLTSNPRNLITWVDTDNDGVVDSGEQMAFSTGNASTLAPYLRANATGTYTATNII
                     DFVHGNQIAGMRDRQVTVNSSLRVWRLGDVVNSTPTIVGAPRERFDILYGDSGYTQFV
                     RRWKDRRMVAYAGANDGMLHAFNVGFYHRGDDASTSGVTEHGRYTRNAVDNSSGPELG
                     EELWGFVPYHLLPQILWLTQPAYTHVSYVDLKPKVTDVRIFSPEAACGGGATPTASGC
                     IHPDGWGTILILGMRFGGSCGSCTAVSGTNNGGPALSVTAKFDGVTTTTRTFYSAYIV
                     LDITDPDANPTVLSAYSSSTLGLTTSYPTIARMSPTGDGKNDHTNAKYFMVAGSGVQG
                     YNGRAVTGAKLFAVQLVLPGTAPTVTILPVGLDTYGGFMADPITFDRDLDFRSDAVYV
                     GRTIAPDAGGAAALGYWWGKFYRLTMGACASAPCTTSTWGVLSGTNRIPTEMVMQVPI
                     GGTPKYLGPVTAGSTVTLDDSGNTWVFFGTGRFFTAADKADQHANYLVGVKDSVLRTG
                     GCVQADAVNCINQNLLDVSSAQICVSCASGNQVQGVGSVTTFSGLMDQIRGNTSLGIS
                     AKDGWVIQLNPNTGSTTLGAERSLVNPTLIGGAIFFPTFTPNNDICVSTGNSSLYAMY
                     YLTGTGYTDPIIGLDANGVSQRSVGIGEGLASSVAIQIGAQPTGMSGFYQSSNSVMSK
                     VSPKAPISLWSQYISWISNRE"
     gene            8475..9551
                     /locus_tag="VFR82_12900"
     CDS             8475..9551
                     /locus_tag="VFR82_12900"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEU4502543.1"
                     /translation="MRTGWASQGGTVTLPERSGRLRTQPSSRDHPITHASTGEDKKLR
                     VFALFGIIGGLLTALALVWGGLSRDKNETASQALGTSNHSPVVKAVTILPSPLNLSGP
                     ISVRVEAQDLDQQTMVFRYRWLVNGHVMSDQTLSTLPAQLIKRGDQVSVEVIPFDGIT
                     EGTPFRSVPVTVTNTAPIVSDVAVDLDHDVQGRRLLAKVNVADPDQDPVTLTYRWRKN
                     ETVLKEGEDNALDLAGLTAKDMVQVEVTASDGAPHGSTTVSGRFTLTNSSPSIVSSPA
                     ASPRGDHYEYLVRATDPDGDPLTYALEVSPPGMTIEAETGQIRWMISQETTGSQRVRV
                     VAKDPQGGFATQDFDLSLNKPTKS"
     gene            complement(9675..>9880)
                     /locus_tag="VFR82_12905"
     CDS             complement(9675..>9880)
                     /locus_tag="VFR82_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502544.1"
                     /translation="FGQLFGEDHQGLTTAVFEAKGSLQNPDVSYLPLESLARGLTGYP
                     RLAIDVLVNTIKLPPTALAYATE"
BASE COUNT         2264 a         2614 c         2838 g         2164 t
ORIGIN      
        1 cggtggacag tcatacaaat tcggcttgtg tagtatccag gagcggatgc aggccttggg
       61 aggatcgttt gacattctat ctgctcctgg gcagggcgtt acggcgacgc tgacccttcc
      121 actgagccaa agagagacga gacctgccgc ttgactggct tggagagagc ttgctgctga
      181 gcaaattgtt cagcgcccat ggcgttcatc tagcttaatt tcatccgacc tgtttcctca
      241 atcgaagtct cgacccataa cctccaacca acacatggcc agctcagaat gctcggcggt
      301 gagtcgagca agaaatccgg cataaatcga tttcccacgg cactgtgaac ctgagtcatc
      361 aatcgctatc agatgctcaa tctctacatc gtatgagaac aaaaactttg acgattttcg
      421 atcctttggt acaaattgcc agaattgctc ggatgtagag tctcgaagtg tttcgcaagg
      481 cgttgcttta tctcaattct taacccggca caagtcctgc tgttatctga ccagtaatac
      541 gggggctacc gaaaatgggt agccagagtc actgagcttt cggcaccatg tcgaagggct
      601 gtaaaaaagg aggcatgtga tgggacgtct tttgtctctc gtagtcacag gggccttttc
      661 tgtttcgctc ctcatggtgg gagcagcgca agcggtctgc gatccactct gttctccgaa
      721 ctcgaataaa agcggcgcag tgagaggttt ggatcgcgcg gatgccgtag ccggcggcgg
      781 cgcggacggg cgtaccaatg cccgcgagaa gcagggcgct gtaacgctac cgcccgatgc
      841 ggataaggat ggggttcctg atgcccagga tcaatgtttg aacgtggccg gaccgggacc
      901 aagcggttgt ccggtagtcg aacttcctcc tccaggggcg tgaccgggtt gtgacggggg
      961 tacgtccaag aaggggcgtc attgggacgc cccttcactc atgttctccc tttcccttcc
     1021 aaggcggatt gcgcatcttc cacgacagtg aatgcactta cagaagtgat caccgaacag
     1081 tagttgctta accaaaaatt gtcgccgatc gaccaaaaaa gcaaaatctt atccatccgc
     1141 caatagtttc ttcctgataa atcagcaatt tgcacatcgc ttgaccaccc tcgcgaaggc
     1201 atgtcccttg ctctcaccct cagagccaag tatggccctc accggaggat taccttctgt
     1261 gtctgctctg gatcgaaata gcggcggctt caccttaatc gaactgatgg tcgtggtggg
     1321 tatcttgacc atcgccgtcg cgctcgcggt accagaattc gttcggtggc atgtgcaatc
     1381 ccaactcagg caggcaacat cagagattgc cacacaactc atgctggcac gcatggcagc
     1441 catgaacagg aaccgaagcg tggatgtcac ggtacagacg accggagcgg ccgttcatat
     1501 ctcggcagtc accccttcct cgggagcggc ggtcatcaaa gacaaggtgt ttccgagtcg
     1561 ggtcacatct gtattgggaa gtcccgtcac ggtgagtttt tcgtcgctgg gtatgcggac
     1621 gaccggaggg acgggcacgc agaccatcgg tgtgtgcgat acgtacgggc gccaatattc
     1681 tgtggcgatc atcccgtcgg gaaaggtcaa ttggtcgatc aattcaagcg gcaccccatg
     1741 tccgtgatgc gagactgcga gatctcatgc gattaaaccg actcaagagc caacaagggt
     1801 tttcgttgtt ggagggaatg ctggccgcgg tgctcttggg cacgggtcta ttggccatgt
     1861 cggcgatgca aggcattgcg atcgtcaaaa atgttgattc gaacgaactc acacgcatca
     1921 ccgcgctcgc gtccgacatg atggaacgga ttcagttcaa ccgacggaat gcaatcagct
     1981 acaacggcat caacacgcag agcgccacca attgcaacac catcagcgcg gcgacacagc
     2041 cgcaggctcg aggagattgt ttgctgtggg acagcctggt cgatgcgacg caataccaaa
     2101 atgtgatggg caccgtcacg gtgtccgata tcatcgcacc gactgccctt gctcagcgga
     2161 atgtggcggt gaccatttca tgggtgggtt cgctcaaaag tgatacgacg gtgaaacgag
     2221 cacggtcatt gaccgtcaat cgagtggttg ccccggaatg acagggaaag atttcgccat
     2281 gcgccgatca accgaacagg ccggattcac gctcattgaa ctgatggtgg gcatggcggt
     2341 gtccgtcatc gtggtcgctg cagcctttac gattctcacg acgacgagca agaccttacg
     2401 agccaacgaa caagtcgttg atactcagca gaacctccgg atggctatgg aaatgctcgc
     2461 aagagatttc aagctggccg gctttggcag tcccccggct cccatcggga attgcacgaa
     2521 cggaattgtc ccggccgatc agaataccgg tggcgccgac acaggcccgg attcggttca
     2581 gttattggtg ccggcaacaa ggtcctctgg cgcgaacagg tggacgctga gactcccgac
     2641 gacggcggga acgacggtga ttacgttatg gcctggagcc gtcactgaca tggtgaattc
     2701 tgggcttacc gcgaacgcgt catacatttc gatcggcggt gcggccactg cccaggtgac
     2761 gggatttaat gcgactgcca gtacgatcaa tgtggcgatt cctccaccgc tctggtttca
     2821 gacagaagat cgggtctatc tactgcaatg cattcgttat cgcgtcgtgc ctgcccccga
     2881 ccccggcaat gtctgtgggg gagtcgcgcc ttgtctgacc agaggagtcg caggcgtcgt
     2941 ggcgggtccc aatgccgagg caccgattgc ggaaggaatc gaggatctcc aactggcgta
     3001 tgcatgcgac ggctgcgtca cagccattaa caccggcatt cccgatcgta tcatcgacga
     3061 tcagaacgct tcgaacacct ttgaccaagc ggatttcctc agtaacttgt cgtggactgc
     3121 tgcgccgctc acgcctgaca agattcaatt ggtgcaggtc gctctcgtgg cgaggcaatc
     3181 gaaaaccgac caaggattta gcgaagcgaa caacacgatg gtcggatcat ccacgctgac
     3241 cgtcacgggg gatcactcgt tacctgctga tgccaatcat cggcgccgca ttttggtgaa
     3301 gactgtggag acccgtaatg tcggtatgta gtcggacacg tctgtcgaaa cgttcctctt
     3361 gctggtcgct cgttttgcgg agcgatcagc gaggcatggc catgttgatc gtcatgatgg
     3421 tgctgcttat gttgacggtt cttggcatcg cggccattac cgtcagcgga ttagggaata
     3481 ccatggcagg attacagcga acaatggaag cctctgccaa cgccgcagag tcatgcctag
     3541 gaaccggcgt caatatcatc caatacgtat tactgacgga aaacgggcgt cagattccat
     3601 ctgcattgct gaacagcact cccggaggac cggttcccag tggaaataag acggtgttgg
     3661 aaagcgaaat tgcaggtaat tcgcagaaca atacggacgt accgattgga cccggaggga
     3721 ctccgggggc tcccaacctt caaatgagcg tgggccctta tgaggtgctc ggagatatcg
     3781 acttcctgta cgtgaagccg agggctggaa ccggacaaat gcaattctca ggttacgagg
     3841 gggccggctc gggcggagga aatgacgttg atgctttctt caggctcacg tgtatcgcga
     3901 acaatgtcgc aactggaaca gagagtcagg tttcggcgat ttatgcctgt tcaatgggag
     3961 gagatggctg tcagaagcag ccatgatttt gtgctggtgg aaaacagtcg acttgtaatg
     4021 gtcggatctg aatctcccat gctggcggca cagcacgccg ctatctgcac aggtcataca
     4081 gggaggacac catgaaacgg gaaagtttta gaaccaggtg gaatcgggtt ctaatcagca
     4141 tttctctgtt tctctcgttg ggaagcccta ttgctgcgtc ggcacagaac atggctgact
     4201 acacgaacta tccagtgttt ctcaatcaga cggttccacc caatattttg ttcatcgtcg
     4261 acctcggcaa cgaaactctt ccggcgggtt acgccggttc gggtcataag tacccaatct
     4321 cattcaaagc atccactccg accgctggga gctatgcgtc aaacgtcacg ttcgatgcga
     4381 ccggtaccaa cgccgatatg gtcgccgtca gtgaccccgg cggaacagcc atgagcacag
     4441 ccacgaccgc ggccccagcc gatatattca attcgagcaa aaactactat ggcatttttg
     4501 atccgtttcg atgttatgcc accggttcga acgactttgt ttacggctca aaaaaaacga
     4561 cgttgagcca ggcgtgtggc accagccatt gggacggcaa ctttttgaac tggctcatag
     4621 gccggaagaa agatttgacc tatcaagtcc tcatgggcgg taccacgaat cctgcgtcct
     4681 cgaacacgga cgggaccgct gactttatct cgagtgagaa gaacacgggc gagggtggca
     4741 cgacagaaag ctgcgcgaat gacaccagat cctgctggcg gttcgtcaaa tatgtgccca
     4801 ctgcaacctt ggccggccgt gtgcccacag gcttgccgaa tccgaccgtt acgggaatca
     4861 atgttggtac tggtacggcg gcagatgccg ggctctggtt tggggccggc gacggcaaat
     4921 tgtacgtgaa cgacaatgcc gacgtagatc ccttcaacac cgcgaatagc aacaaataca
     4981 cgctgcgcgt ggacctcacc acagagccca atataccctc cggaacaggt aatttgagcg
     5041 gcagctgcga tgagggcgat acggctaatt ttgccggaca catgatctgc tatcgcaagg
     5101 atcgttcatt aggtctgttc cagaagatgc ggaccgataa catgcatgtc ggggtaatgt
     5161 tcgccaatgc cttgagcggc aagggtggca aggtgatgtt caacttcgac gagagtttca
     5221 actcctcagc gatcacgaac cttcgcaatg aacacatcca acccaagtcg ccgctttctg
     5281 aagccttgta tgagggcttg tgtctgttcc ggaaatctca aggaccttgc tacaacaatt
     5341 caggcgcatc ggcaacgggt tacacgtcaa gcacgggagt ggcaagcgat ccgttctatt
     5401 tcacctcgtt cggccagagt ttgcggtgct gtaagagctt cgtgctcatg atcagtccgg
     5461 ggcagggagt tgacgacggc aacgctcccg atctgcaaac acccttcggg aatctattta
     5521 ccggtaccaa tatcggggtg gtgacgaccg gtgccgcggg tgaccggtta gacgacgtcg
     5581 cgttttatgg gcagacgcac gacatcagag agcaggcatc ccctgtgggt cttgcgggta
     5641 ctcagagcgt gacgttttat gccgtcaatg cgatgggcgg acaggtcggc tcgacactct
     5701 tggcctccgc ttccaagtac ggcggattcg aggatcgaaa tagtaacgga acggctgatc
     5761 tcacggggca atcatgcact tatcccgcag gatcgagcct tggaactggg acgagtacca
     5821 gcaacgccga atgggacgtc gatcaaaact gtgtgccaga cacgttcttt gatgcgtcgg
     5881 aaggcggcga tctcgaagcg caaattaacc tggcgatttc ctccattctc aagaaatccg
     5941 cgtcgggaac gtccatttcg gtgttggcct cctcttcaac gggagaaggc tctatttatc
     6001 aagccttctt ctatccaagt accttcgaag gcaccaaaga gatcaagtgg accggtttcg
     6061 tgcagggtct ctttgtcgat acctacggca atttgcgcga agatcgagga ggcagcgggg
     6121 gcagtcccga cggcaggctt gtatacacgg atgacaacat cgtccaaacc aggttggatg
     6181 ccgccaccgg cgatgtaaaa gtcgatcgct tcgtcgatgc ggatggcgac ggcaaggcgg
     6241 atagtacgac tccctatgag tcggtcggac ttcgtgagat gcaaggcatt tgggaggctg
     6301 ggaagaagct ggcattacgc gatctaacca gcaatccaag aaatctgatc acctgggtcg
     6361 atacggacaa cgacggggtc gtggatagcg gggagcaaat ggcgttcagc acgggaaatg
     6421 cgtccacgct cgcgccttat ctcagagcta atgcaacagg cacctacacg gcgacgaata
     6481 tcattgattt tgtgcacggc aaccaaatcg cgggcatgcg ggaccgtcag gtgacggtga
     6541 atagctcact gcgagtctgg cggctcggcg acgtggtgaa ctcgacgccg accattgtag
     6601 gggcgccccg tgagcgtttc gatatccttt acggggatag tggttatacc cagtttgtaa
     6661 ggcggtggaa agatcgtcga atggtggcat atgccggcgc caacgacggc atgctgcatg
     6721 ccttcaacgt cggcttctat catcgtggcg atgatgcgtc gacctcaggg gtgacggaac
     6781 acggaagata cacgagaaat gcggtcgaca acagcagtgg tcccgagctc ggggaagaat
     6841 tatggggatt cgttccctat catctgcttc cccagatcct ctggctcaca cagccggctt
     6901 atacccatgt gtcatatgtt gatctgaaac cgaaagtgac tgacgttcgg atcttctctc
     6961 cggaggccgc atgtggaggt ggtgcgacac ccacggcttc gggttgtatc catccagacg
     7021 gatggggcac gattttgatt ttgggaatgc ggttcggcgg aagctgcggg tcctgtaccg
     7081 ccgtctctgg taccaacaat gggggaccgg ctctgtccgt caccgcaaaa ttcgatggtg
     7141 tgacgacgac gactcggaca ttttacagcg cgtatatcgt gttggatatt accgatcccg
     7201 acgcgaatcc gacggtgctg tccgcgtatt cgtcgtcaac actcggattg acgacgagct
     7261 atccaaccat tgctcggatg agccctacag gcgatgggaa aaacgaccat acgaatgcca
     7321 agtatttcat ggtggccggc tcaggcgtgc aagggtataa cggaagggcg gtcaccgggg
     7381 caaagctttt tgcggttcaa ctggttctgc ctggaacagc tccgaccgtg accatcctac
     7441 cggtcggcct cgacacctat ggcggcttta tggccgatcc gatcacgttc gaccgtgatt
     7501 tagatttccg ctcggatgcg gtctacgtcg gccggacgat tgcgccggac gcaggaggtg
     7561 ccgcagccct cgggtattgg tggggaaagt tttatcgtct tacgatggga gcttgcgcat
     7621 cagccccctg cacaacttcc acttggggag ttctatcagg gacaaaccgc attccgactg
     7681 aaatggtgat gcaagtgccg atcggcggaa cgccgaagta tcttggaccg gtaacggccg
     7741 ggtccacagt gacgcttgac gactcgggca atacatgggt gttttttgga acgggacgct
     7801 tctttaccgc tgccgacaaa gccgatcaac atgccaatta cctggtgggc gtcaaggatt
     7861 cggttctacg aacaggcggc tgcgttcagg cggatgcggt caactgtatt aatcaaaatc
     7921 tgttagatgt gtcgagcgcg cagatttgtg tctcgtgtgc atcgggcaat caagtccaag
     7981 gggtcgggtc ggtgacgacg ttttccggac tcatggacca gatacgcggg aatacgtcac
     8041 ttggcataag tgcaaaggat ggatgggtga tccagctcaa tcccaatacc ggttcgacaa
     8101 cgctgggagc ggagcgatcc ctcgtcaacc cgactttgat tggcggcgct atctttttcc
     8161 caaccttcac gccgaataat gatatctgcg tatcgacagg gaatagctcc ctgtacgcga
     8221 tgtactacct cacaggaacc gggtataccg atccgatcat cggattggat gctaacggtg
     8281 tttcacagcg aagtgtcgga attggtgagg gtttggcctc atctgtcgcg atacagatcg
     8341 gtgctcagcc gacgggaatg tcgggcttct atcagtccag caactccgtc atgagcaaag
     8401 tgtcgccgaa ggcaccgata tcgctgtgga gtcaatacat ctcatggata agtaaccggg
     8461 aataaacaga gcggatgaga accggctggg catcgcaagg tgggacggta actcttcctg
     8521 agcgaagtgg gcgcttgcgt acgcagccct cttctcggga tcatccaatc acgcacgcct
     8581 caacaggcga agacaaaaaa ctccgtgtct tcgcgctctt cggcatcata gggggactgt
     8641 taacggcttt agcgctcgtc tggggtgggc tgtctcgtga caagaacgag acggcaagtc
     8701 aagctttagg cacatcgaat cactctcccg tcgtcaaggc agtaaccatc ctgccgagtc
     8761 cgttgaactt gtctgggcca atcagcgtca gagtcgaagc gcaggatctt gaccaacaga
     8821 ctatggtttt ccgctatcgt tggctggtca atggccacgt catgagtgat caaacgctga
     8881 gcacactacc tgctcagttg atcaaacgtg gtgaccaagt ctctgttgag gttatcccgt
     8941 ttgacggcat caccgagggg acaccgtttc gatccgtgcc ggtgaccgtc acgaataccg
     9001 cccccatcgt ctcagatgtc gcggttgatt tagatcacga tgttcaaggc cgtcgactct
     9061 tggccaaggt aaatgttgcg gaccccgatc aggatccggt gacgctcacc tatcgatggc
     9121 ggaaaaacga gacagtcctg aaggaggggg aggacaatgc gttggatctt gctggtctca
     9181 cggctaagga catggtccag gtggaggtga ctgcatcgga tggtgcacca cacggaagta
     9241 cgacggtgtc gggacggttc acgttgacca attcgtcccc atccatcgtg tcaagtccgg
     9301 cggcatcgcc gagaggcgat cattacgaat atctggttcg tgccactgat cctgatggag
     9361 accctctcac ctatgcgctg gaagtgtcgc ctcctggaat gaccatagaa gccgagaccg
     9421 gccagattcg ctggatgatc agtcaggaaa caactgggag tcagcgtgtc agagtcgttg
     9481 ccaaagatcc gcagggtggt ttcgccacgc aagattttga tctatcgctg aacaaaccga
     9541 ccaaatccta gtggatgtgg actagatgct gcacacgaga aactcaccag ccggaaaatc
     9601 tcttcacaca aatagccgtg cctcgtgcat ctgccaccaa cgcaaaggtg ggtgctttac
     9661 gcgtaagcaa tcgctcactc tgtggcgtag gcaagcgcag taggaggcag tttgatggta
     9721 ttgaccagca cgtcgatggc cagacgggga tagccagtca gtccccttgc gagcgattcc
     9781 agaggtaggt atgagacgtc cggattctgg agcgatcctt tcgcttcgaa gaccgcggtc
     9841 gtcaatccct gatgatcttc gccgaatagt tggccgaaga
//
LOCUS       DASTQY010000397         5545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1133271, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000397 DASTQY010000000
VERSION     DASTQY010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133271"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VFR82_12910"
     CDS             complement(<1..906)
                     /locus_tag="VFR82_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502545.1"
                     /translation="MPVSVTSPSLQALRALLLFVFFIFVPTFLSAALEERASSSPPDW
                     HKLINLHHDRLAKVESDQAALDLFVAAIGRAAEVNDVAATIAVKGLSPHVTKDLMVPE
                     ITKSAQRLVAALVAWDVADRMMHALEEKSARTDVLTTPSQAAWLTANGPFASLQEAVR
                     QWDSGDATAAAGVAAGQLAQQGAQQATEEWWRLRTWKDRVRTARGQSRLCGTWHWVVH
                     NHQRHHEERKLSLVFPPPGTEGMAIPGLTETVVLGDAVYLRWETDGRIQEDSLLFSKE
                     GQRLEGSFVNSQGGWGSITGKRTATC"
     gene            complement(944..1741)
                     /locus_tag="VFR82_12915"
     CDS             complement(944..1741)
                     /locus_tag="VFR82_12915"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU4502546.1"
                     /translation="MVSTTAPFTLQQIGTGTSRFPSGVALPTITDASVDPYIRTRPIQ
                     TVQVECIEFWPQEKGTYPGIVLLHEWWGLNSQIKDLGARLACEGYGVIIPNLYGRIGG
                     MVTGNAEVAEALMGKLNESLALQDINSCCEFLNTREFIKRNIHGVVGYGMGGTLAMKF
                     ACQRKRLRGAVSYYGRMIQPDSALEKALCPILYHYAEHDPWVSGRDVQRLRELAAEYK
                     KSIEIQSYSDAPHAFSDETRPENYREAAASEAWATTAMFLKKHLQGS"
     gene            1874..2872
                     /locus_tag="VFR82_12920"
     CDS             1874..2872
                     /locus_tag="VFR82_12920"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU4502547.1"
                     /translation="MHSSIGSFSTANLGIGLIGVGRHGSRYVQHLVRDLPDVTLAAVC
                     RRSRGGVFLDATIPVYDDYRAMISDPRVHAVVVVTPPSLCYDICLAAVHARKPILIEK
                     PLALNGSQARTMVAAASQSGVLLMTAHTLRFDPVIKMLREQIHTIEPLQSACLTTHID
                     TKPNVMSVGDGSVKVGALLELGIHLLDLVRFLTREDIIEVQCTMTPLPSVAPETQVEV
                     RLRTSSSIACTLDIARVEVQRTGRAVWIGEKGTLTADWVSRSLTRTTEDGRLETWTLE
                     FKPTVLATLQAFVYAIRTATVPPITGLDGCLAVEAADACYRSAANHGAVTLVGSIR"
     gene            complement(2922..3158)
                     /locus_tag="VFR82_12925"
     CDS             complement(2922..3158)
                     /locus_tag="VFR82_12925"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEU4502548.1"
                     /translation="MATKAYILIKVKTGRTKDVLQALKKLAGVEQAHSCFGQPDIFLF
                     ISVQDERSLSDVVITKIHQIEGVEETDTHIVADS"
     gene            3887..5413
                     /locus_tag="VFR82_12930"
     CDS             3887..5413
                     /locus_tag="VFR82_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEU4502549.1"
                     /translation="MLAKVRTVALTGLDAHLIDVEVDIAGGLPQFSVVGLPDATVRES
                     RDRVRSALKNTGFHFPAKRITVNLAPADIKKEGAGLDLAIAIGILVAEEVIAAERLDR
                     RVLVGELSLDGQIKPVSGALSIGLACRNHSLLLPAQNGPEAAMIDGVVVYGLHTLPEA
                     VAFLRGECELEPHDADRTKWFSPAPAEDEDYGDVRGQDHAKRALEIAAAGGHNVLMVG
                     PPGSGKTMLAKRLPTILPLMEPDEAIETTRVHSVAAQLPADRPLLTVRPFRAPHHSIS
                     DAGLIGGGTIPRPGEVSLAHNGVLFLDESPEFRRPVLDGLRQPLEEGAVTLTRISGSL
                     RFPARFMLIAAMNPCPCGYYGDRSRECVCTPYQIRRYRARLSGPLQDRIDIHIEVPPV
                     PVRDLQDSNAAPEGSISIRTRVIEARKRQAGRYGSDGIHTNAQLKPRLVKRYCGLDTK
                     GQDLLEHSMMRLGLSARAHGRILRVARTIADLAGSDNIQAVHVAEAIQYRSLDRRVEF
                     "
BASE COUNT         1156 a         1667 c         1554 g         1168 t
ORIGIN      
        1 gcaagtcgcc gtgcgctttc ctgtaatgga accccaccca ccttgggaat tgacgaaact
       61 cccttccaac cgctgaccct ctttggaaaa aagcaggctg tcctcctgaa tccgtccgtc
      121 tgtttcccat cgcagataga cggcatcacc cagtaccaca gtttcagtga gaccaggaat
      181 tgccatgccc tctgtccctg gcggcggaaa cacgagggat agcttacgct cttcgtgatg
      241 ccgctgatgg ttatggacga cccagtgcca tgtcccgcag agacgagact ggccgcgtgc
      301 cgtgcgcacg cgatccttcc acgttctaag ccgccaccac tcttcggtgg cctgctgagc
      361 gccctgctgc gctagctgtc cggccgcaac gccggcggcg gccgtcgcgt ccccgctatc
      421 ccattgtctg accgcctcct gcagggatgc aaatgggccg tttgccgtca gccatgcagc
      481 ttgggagggc gtcgtcagga catcggtccg ggcgcttttc tcttccaaag cgtgcatcat
      541 ccggtccgcg acgtcccagg cgaccaaggc cgcaaccaat cgttgcgcgg atttcgtgat
      601 ttcgggcacc atcaggtctt tcgtgacatg aggagagagt cccttgacgg caatcgtcgc
      661 cgccacgtcg ttcacttcag cagcccggcc gatcgcagcg acgaacaggt cgagcgccgc
      721 ttgatcagat tcaactttgg caagccgatc atggtgcagg ttgatgagct tgtgccaatc
      781 cggcggagac gaggaagcgc gttcctccaa tgcagcggaa aggaaagtcg ggacaaagat
      841 gaagaacacg aacaaaagca acgcccgtaa ggcttgcaga ctgggcgatg tgacagagac
      901 gggcatggtg acggacaggc gattcgcggc agagggacga cctttaggac ccttgaaggt
      961 gctttttcaa gaacatggcc gtcgtcgccc atgcttcgct tgctgcggct tcccggtagt
     1021 tttcaggtct cgtttcgtca ctgaaagcat gcggcgcatc ggagtaggac tgaatctcta
     1081 tcgacttctt gtattcggca gcgagttccc gcagacgttg aacatcccgc cctgacaccc
     1141 aaggatcatg ttccgcataa tgatagagga tgggacagag cgctttctcc agagcggagt
     1201 ctggctgaat catgcgtcca taataagaca cagccccccg caggcgcttc cgctgacagg
     1261 caaacttcat cgccagcgtc ccgcccattc catatcccac caccccgtga atgttccgct
     1321 taatgaactc gcgggtgttc agaaactcac agcacgaatt gatatcttgc agagccagcg
     1381 attcgttcag ttttcccatc aaggcctcgg ccacctccgc attgccggta accattccac
     1441 cgatgcgacc gtaaagattg gggatgatca cgccgtagcc ctcacaggcc aggcgggcgc
     1501 ccagatcctt aatctgggag ttcagtcccc accattcgtg caagagtacg atgccaggat
     1561 aggtgccttt ttcttgcggc caaaactcaa tgcactcgac ctggacagtc tggatcggcc
     1621 gcgttctgat ataggggtcg accgacgcat cggtgatggt aggcagcgcc actccgctgg
     1681 gaaaccgaga ggtccctgtg ccgatttgtt gtaacgtaaa cggagcagtc gtcgaaacca
     1741 taatccggga aactccctac cagagcagaa ttgcgccata caaatcctgc gcaacttcat
     1801 cgtactatag ggaccgctcc gccggcttgt caatgtaacc tcaaaggcag tagtctgcgt
     1861 caggtttttc gccatgcatt cttcaatcgg ctctttctcg actgcaaatc tcggcatagg
     1921 cttgatcggt gtcggtcggc atggcagccg ctatgtgcag cacttggtcc gcgatcttcc
     1981 tgacgttacc ctcgctgcgg tgtgccgcag aagcagaggc ggcgtgtttt tggacgccac
     2041 cattcccgtg tatgacgatt atcgagccat gatttcggat cctcgcgtgc atgcggtcgt
     2101 tgtcgtgact ccaccctctc tctgctacga tatttgcctg gcggcggttc acgcccgaaa
     2161 accgatcctg attgaaaagc cgttggcgct caatggatct caggctcgca cgatggtggc
     2221 cgcggccagt caaagcgggg tacttttgat gacggctcac acgttgcgat ttgaccccgt
     2281 cattaagatg ctgcgagaac aaattcacac cattgagccg ctgcagtcgg cctgtctcac
     2341 cacgcatatc gacacaaaac ccaacgtcat gtccgtgggc gatggatcgg tgaaggtcgg
     2401 tgcactgctt gaactcggga tccatctgct tgacctcgtc cgctttctca cgagggagga
     2461 tattatcgag gtgcaatgca cgatgacccc cttgccctca gtggcaccgg aaacccaggt
     2521 ggaggtacgg ttgcggactt ccagcagcat tgcctgcacc cttgacatcg cacgcgtcga
     2581 ggtccagcgc acgggaaggg cggtgtggat cggagagaag ggcacactca cagcggattg
     2641 ggtgtcgcgg agtctcacga gaactacaga ggacggacgc ttggaaacct ggacgcttga
     2701 gttcaaacca accgtcctgg cgacgttgca agccttcgtg tacgccatac ggaccgcaac
     2761 ggtcccgccg attaccggcc tcgatggctg tctcgccgtc gaagcggccg acgcctgtta
     2821 tcgttcggca gccaatcatg gtgccgtaac actggtcggg tcaatccgct agagaacgcg
     2881 agtccaacgg cgatccgatc cgtcaacaaa acgcggcagg actagctgtc cgccacgatg
     2941 tgcgtatcgg tttcctccac cccttcaatc tgatggatct tcgtgatgac cacgtcggag
     3001 agagagcgct catcctggac gctgataaag agaaaaatgt ccggctgccc gaagcaggag
     3061 tgcgcctgtt ccactcccgc aagtttcttc aaggcctgca acacgtcttt cgtccggccc
     3121 gttttcactt tgatgagtat gtacgccttg gttgccatgg ggtccctcct ttcgatcaaa
     3181 gtgtttcccg cccggcatgc tccgtcggtg ctccgcaaaa atccgatgga tatcgcgccg
     3241 ctgctggata aatgcggcac gcgccttggc atagagctca cacagccatt ccaaatcgac
     3301 cagtcgcatg ttcacacgag cgaagggtgc tgctaacctg tcccgttctt ctctcgcggt
     3361 tgtagcataa cggtcgcata ccgcctccac gacctgagaa ctccaccctc cctgacggaa
     3421 ttcagttccc gcttcctccg cccgatcggc aaacttcgac gtcaacgcct gatcgagaaa
     3481 tcgacgcacg gcgggatcgg tgctcttcat aaacacacct tgaaactgaa tcacgatctg
     3541 aatcacatgg ttcgcccccg gagtcccttc agggggaaga atgtcggcat cttgcagcgt
     3601 ggctaagagt gccatcgaag cccccaccgg tccggatgaa gtcccgacct gcgccggggc
     3661 catagagaga accgcggctc cctgcagcaa gacgagcgac aaaacgatgc caggccaatg
     3721 catgccgcgg attatacaga aggggactca tgtgcaccag cttgcgatca acagcaagaa
     3781 tccggttggc gaatggaagc atttcacctt gctgggcgtg ctttttcttg aatccagtac
     3841 agtcgctttc tataattcag cacctctgtg actgcatgga gttttcatgc ttgcgaaagt
     3901 gcgtaccgtc gcgctgaccg gactcgacgc gcatttgatc gacgttgaag tggatatcgc
     3961 cggcgggctg ccacagtttt ctgttgtagg attgccggat gcaaccgtcc gtgagagccg
     4021 cgaccgagtc cggtccgcac tcaagaacac aggttttcac ttccccgcaa aaagaatcac
     4081 agttaatctc gcgcctgccg acatcaagaa agaaggggcg ggcctcgatt tagcaatcgc
     4141 gatcggcatt ctggtcgcgg aagaagtcat tgcggctgag cggctcgacc gtcgcgtgct
     4201 cgtcggtgaa ttgtctctcg acggacagat caaaccggtg tccggagcac tttcaatcgg
     4261 tctggcctgt cgcaaccaca gtctcttgtt gccggcacag aatggacctg aagccgcgat
     4321 gatcgacgga gtggtcgtgt atggtctgca cacgctacct gaagcggtcg cattcctcag
     4381 aggtgagtgt gagctcgaac cacatgacgc cgatcgtacg aaatggttct ccccggcgcc
     4441 tgcggaagac gaggactacg gtgatgtccg tggacaggat cacgccaagc gagccttgga
     4501 aatcgccgct gcgggcggac acaacgtcct catggtgggt ccgccaggct cgggcaagac
     4561 gatgttggcc aaacgtctgc cgactattct tcctctcatg gaacccgatg aagcgatcga
     4621 aaccactcgc gtccacagcg tggccgccca gcttcccgcg gaccgtccac tgctgaccgt
     4681 ccgtcccttc cgcgcgccgc accatagcat ttctgatgcc ggcttgatcg ggggcggaac
     4741 catcccccgc ccaggtgaag tctctctcgc tcataacggc gtcctgttcc tggacgaatc
     4801 gccagaattc cggcgacccg tccttgacgg ccttcgccag cccctggaag aaggcgctgt
     4861 caccttgacg aggatcagcg ggtcgctccg gttcccggct cgattcatgt tgattgcggc
     4921 gatgaacccc tgtccatgcg gctattatgg ggaccgctca cgggagtgtg tctgtactcc
     4981 ctatcaaatc cggagatacc gcgcccggct ctcaggccca ctgcaagacc gtattgacat
     5041 tcacatcgag gttccaccgg tgccggttcg cgatcttcaa gacagcaacg cagcacccga
     5101 gggctcgatc agcattcgca cacgagttat tgaggcacgc aaacgccagg ccggccgcta
     5161 tggatcggac gggattcaca caaatgccca gttgaagcct cgactcgtga agcggtattg
     5221 tgggctcgat acgaaagggc aggatcttct ggaacacagc atgatgagac tgggcctctc
     5281 ggcgcgcgcg catggtcgga tcctcagggt cgcgagaacc attgcggatt tggcaggctc
     5341 agacaacatt caagccgttc acgtggccga agccattcag taccgcagct tggaccggcg
     5401 tgtggaattc tagaatcctg tgaccgccgc tatgaaagtg ttcgtctggt ggtttctcgt
     5461 cggagcgacg atggcgctcg cggtcatcat gttgcaaggc ggcatgcggg aagtgatgca
     5521 ggcgcaaggc tccgtctggg aatta
//
LOCUS       DASTQY010000398         4679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_87701, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000398 DASTQY010000000
VERSION     DASTQY010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87701"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VFR82_12935"
     CDS             complement(<1..599)
                     /locus_tag="VFR82_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4502550.1"
                     /translation="MKVALIGATGFVGSALLKEALDRGHEVTAIVRNPDKLQSHPKLH
                     PQTGDIYNADEVARLVAGHDTVISAFNPGWSDPDIYNQQVKGTEAIIKGVKKAGVKRV
                     LWVGGAGGLEIKPGVQSVDTAEFPKEWKQGSLATREALNLLHKESSLDWSYLSPSADL
                     SPGQRTGKFRLGKDQLLKDAHGKSHISTQDYAMAMIDEA"
     gene            complement(1098..2228)
                     /locus_tag="VFR82_12940"
     CDS             complement(1098..2228)
                     /locus_tag="VFR82_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEU4502551.1"
                     /translation="MKTAAKLQTREIKAAVIRKKGGPFEIENLALEEPRSDEVLVRIL
                     ATGMCHTDMVARDQLYTVPMPVVLGHEGAGVVEQVGASVKKIAPGDHVVLTYMWCGHC
                     KPCLSGALTYCENFYPLNFGGAREDGSTATHDKEGAVRDHFFGQSSFGTLALAHERNV
                     VKVPSTAPLELLGPLGCGIQTGAGAVVNALKVRPGSSFAAFGGGAVGLSAVMAARVAG
                     ATTIIAADVVPSRLALAKELGATHTVNSRETDPIEAVRKITGGGVDFTLESSGRPAVL
                     RQAIDALAIKGVCGIVGAPALGTEASFDVNGVMTTGKRIIGIIEGDSMPDLFIPSLVE
                     LYLQGRFPFDKLVKFYTLDQINQAAEDSEKGVTIKPIVRFGS"
     gene            complement(2241..3425)
                     /locus_tag="VFR82_12945"
     CDS             complement(2241..3425)
                     /locus_tag="VFR82_12945"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU4502552.1"
                     /translation="MPESVFYRDLAYVFVAALLGGMVARKLRQPLILGYVLGGILIGP
                     FTPGPTVSEVHALEVIAEIGVILLMYSIGMEFSVKDLLQVKWVSLVGGPLGILAVIGL
                     SIAVGQLMEWSTLQSISIGAVVSLASTMVLSHMLLDRGELHSQHGRAMIGITLVDDLV
                     FVVLVVLLPTLATVSTGGVSDIALAFGKAVLILVPIVFIAVKLVPSLLSRVDKMKSQE
                     LYVLVVLALGFATSGVTQALGLSLALGAFLAGLIVSDSEHRQQTLTLVLPFRDASAAL
                     FFVTIGALMNPRALISHPVLLGAIVALIVVGKFVIWTAVVRLFQYPLHTAVLVGVGLT
                     QIGEFSYVLVRVARDAGLVGPDVYGATLAASLLTILLNAFLMHAVPSLIARRRPVLSA
                     TS"
     gene            complement(3643..>4679)
                     /locus_tag="VFR82_12950"
     CDS             complement(3643..>4679)
                     /locus_tag="VFR82_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU4502553.1"
                     /translation="VLANGEILRTAMGGLPKSNAWQIFKWGYGPYLDGIFTQSNYGIV
                     TKFGFWLQPAPPVYKPFMIQYQDEEDVAEIVEIMRPMRLNGVIPNSGVIAHALYEAPL
                     KAKRSDYVSGPGSISDEAVRKLVRDHNMGIWNVTAALYGTQEQVDVNWKIVTEAFGKS
                     GKAKFITEQDAGDDPQFGYRAVLMRGGMDMREFKLYNWRGGGGSLWFAPVSQARGSET
                     LKQMKLAKQILSRHGLDYAGEFIVGMRDMHHILDMLYDRTDPVMAKAAYQCFDELITE
                     FSALGYGSYRTNTAFMDKVAETFGPVQRSVNKKLKKALDPNGILAPGKSGIRLGKTRS
                     VFQFGKSFKK"
BASE COUNT         1034 a         1402 c         1239 g         1004 t
ORIGIN      
        1 tccgcttcat cgatcatcgc catcgcatag tcctgagtcg agatgtggct cttgccgtgg
       61 gcgtccttca gtagttggtc ctttccgagc cggaactttc cggttcgttg gccgggggac
      121 aaatcggcgg agggggagag ataggaccaa tccagacttg attctttgtg cagcaaattc
      181 aacgcctctc gagttgccaa tgaaccctgt ttccactctt tgggaaactc cgcagtgtcc
      241 accgactgaa ctcccggctt gatctccagg ccgcccgctc cacctaccca gaggacgcgt
      301 ttgacgccgg ccttcttcac gcctttgatg atggcctctg tccctttcac ctgctgattg
      361 tagatatcag gatcgctcca cccaggattg aaggcgctga tgacggtatc atggcctgcg
      421 actaaccggg ccacctcatc tgcgttgtag atgtctcctg tttgaggatg gagcttcgga
      481 tgcgactgta gtttgtcggg attccgtacg atcgccgtga cctcatggcc gcgatccaag
      541 gcttccttca aaagcgccga gccaacaaag ccggtggcac cgatcaatgc aaccttcatg
      601 gtgcgcctcc tctaccgtga atttacgccg aaacggaacg agtccgttga tacgatcccg
      661 gaaggttcgt ggttccggtg gcacgacccg acagcgcgag catcatccat ccccagccat
      721 ggaagatcat atcgatgccg atgcacaccc gatgaaccag aggcccaatt ccggccattc
      781 cgtccacaga agcaggctca agagtacgct aatcgtgcca cccacgagag cccaactcca
      841 attgggaaat cgcaaggtcg gcatcctcac ctcatcctca cggacgaagc acaattttat
      901 acggtggcat agtgcgagct gtacacaatt gctcacttgc cgaatctccg ttgaattttc
      961 ctctgctaac aacgctttga tggtcttcga ttcggcagga atggaagttc ccaggctcat
     1021 gtggaccagg agccgattta ggcacagcat gattttttct ttccaacggc gcgtcgatat
     1081 gacgtgcgcg cctgttttca tgacccgaac ctgacgatcg gcttgatcgt cacgcccttc
     1141 tcactgtctt cggccgcttg gttgatttga tcgagggtat agaacttgac cagcttgtcg
     1201 aatgggaatc gtccctggag gtaaagttcc accagactcg ggataaacag atccggcatg
     1261 ctgtcgcctt cgatgatacc gataatccgc ttaccggtcg tcatgacccc gttcacatcg
     1321 aagctggctt ccgtccccag cgctggggca cccacgatcc cgcacacgcc tttaatcgcc
     1381 aaggcgtcga tggcctgacg cagcacggcc ggcctgccgc tcgattcgag cgtgaaatcg
     1441 acgccgccgc cggtgatctt gcgcacggcc tcgatcggat ccgtctcgcg gctgttcacg
     1501 gtatgggtcg ccccgagctc tttggccaat gccagccggg acgggacgac atcggcagcg
     1561 atgatcgtgg tcgcgcccgc tacgcgcgcg gccatcacgg cgctcaagcc gacggctccg
     1621 cctccgaacg cggcgaagct gctgccgggg cgaaccttca aggcattcac gacggcgccg
     1681 gcaccggttt ggatcccgca accaagcgga ccaagcaatt ccagcggcgc agtcgatgga
     1741 actttgacga cattgcgctc gtgagcaagg gccaaggtgc cgaatgacga ctggccgaag
     1801 aaatggtccc ggacagctcc ctccttatca tgggttgcgg tacttccatc ctcccgcgcg
     1861 ccgccgaaat tcaatgggta gaaattctcg caataggtca atgcgccgct caaacacggt
     1921 ttgcaatggc cgcaccacat gtacgtcagt acgacgtggt cgcccggtgc gatctttttg
     1981 acgctggcgc cgacctgttc cactacaccg gctccttcgt ggccgagcac gacaggcatg
     2041 gggaccgtgt atagttggtc ccgtgcgacc atatcggtat ggcacattcc ggtcgcgagg
     2101 atccgcacca gcacttcgtc ggagcgcggt tcttccaatg caaggttttc tatttcaaag
     2161 ggcccgcctt tcttgcggat gactgccgct ttaatctctc tcgtctgtag cttggctgcg
     2221 gtcttcatct cgttactcct tcatgacgtt gcacttagga caggccgccg ccgcgcgatc
     2281 aagcttggca cggcgtgcat gagaaaggcg ttcaacagga tcgtcaagag agaagcagct
     2341 agcgttgcac catacacatc gggtcctact aacccggcgt cccgagccac tctcacaagg
     2401 acataggaaa actcaccaat ctgcgttaga ccgactccaa ccagaacggc ggtgtggagc
     2461 ggatactgaa agagacgaac caccgcagtc cagatgacga acttgccaac tacgatgagt
     2521 gccacaattg cgccgaggag gaccggatgg gaaatgagcg cgcgaggatt catcagtgcg
     2581 ccgatcgtaa caaagaataa tgcagccgaa gcgtctctga acggaagtac cagagtgaga
     2641 gtttgctggc ggtgctcgga gtcgctgact atcagtcctg ccaggaatgc cccgagtgcg
     2701 agcgacaatc cgagagcctg tgtgacgcca gatgtcgcga atcccaaagc gagtacaacg
     2761 agcacataca gctcttggct cttcatctta tcgacacgtg acaataatga cggaacaagt
     2821 ttcactgcga tgaaaacgat gggtacgagg atcagcacag ctttgccgaa ggcgagggca
     2881 atatcggaca ccccgccggt gctgactgtc gcgagtgtcg gcagcaggac caccaagacg
     2941 acgaaaacca gatcgtccac caatgtgatg ccgatcatcg ctcggccatg ttgagaatgc
     3001 agctcacccc tatcgagcag catgtgggat agcaccatcg tactcgcgag ggacaccacg
     3061 gctcctatgc ttatgctttg taacgtactc cattccatga gctgtcccac ggcgatgctg
     3121 agtccaatca ccgccaaaat tcccagcggc ccaccgacca gagaaaccca tttcacctgg
     3181 agcaggtctt tcactgagaa ttccatcccg atggaataca tcaacagaat cacaccgatc
     3241 tcagcgatga cttcgagggc gtggacctcc gaaacggtcg ggccgggtgt aaatggaccg
     3301 atcagaatac cgccaaggac atagccgagg atgagcggct ggcggagctt gcgagccacc
     3361 ataccaccaa gcaaggcagc gacaaacaca taggccaggt cacgatagaa gactgattcc
     3421 ggcattatat atttactgag tgttgcccga tttccaggat taactcgtga ttatttcttc
     3481 atgccatgca tacgtgaggt ctgcagtgcg acgaaacacc cggcggcagc gtgcatcgtg
     3541 gagtgataca tctccctctt gaggggtttt gattctctga gggagcgaaa gcaaccgcca
     3601 caatccctct aggctctgcc accgaatgat tggataccca tcttattttt tgaaagactt
     3661 tccaaactgg aaaaccgacc tagtttttcc gaggcgaatt cctgattttc ccggtgccag
     3721 aatgccattg ggatccaatg ctttcttcaa ctttttgttc actgagcgct ggacggggcc
     3781 aaacgtctcg gccaccttgt ccataaatgc cgtattcgtt cgatagctgc catagcccag
     3841 agctgagaac tcggtgatca attcatcgaa acactgatat gccgccttcg ccatgaccgg
     3901 gtccgtccgg tcatagagca tgtcgaggat atgatgcatg tcccgcatgc cgacaatgaa
     3961 ttccccggcg taatcgaggc cgtgccgact gaggatctgc tttgcaagtt tcatctgctt
     4021 gagcgtctcg ctcccgcgcg cttgcgaaac cggcgcaaac cacaaagagc ctccgccgcc
     4081 gcgccagttg tagagcttga attccctcat gtccatgccg ccgcgcatca ggacggcccg
     4141 atagccaaat tggggatcat cgccggcatc ctgctccgtg atgaacttgg ccttgccgga
     4201 tttgccgaag gcttcagtca cgattttcca gttcacgtcc acctgctcct gcgtgccata
     4261 caacgcggcc gtgacgttcc agatgcccat gttatggtcg cgtacaagct tgcgcaccgc
     4321 ctcatccgag atggagccgg gacccgagac atagtcgctc ctcttggctt tcaacggcgc
     4381 ttcatacagc gcatgcgcga tcacccccga attgggaatg acgccattga gacgcatcgg
     4441 ccgcatgatc tccacgatct ccgcgacgtc ctcttcatcc tgatattgaa tcatgaacgg
     4501 cttgtacacc ggcggcgcag gctggagcca aaacccgaat tttgtcacga tgccgtaatt
     4561 ggattgggta aaaatgccat ccaggtacgg cccgtagccc cacttaaaga tctgccaggc
     4621 attggatttc ggcaaacccc ccatggcggt gcgtagaatc tcgccgttcg ccagcacca
//
LOCUS       DASTQY010000399         3865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1242572, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000399 DASTQY010000000
VERSION     DASTQY010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242572"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..966
                     /gene="gap"
                     /locus_tag="VFR82_12955"
     CDS             1..966
                     /gene="gap"
                     /locus_tag="VFR82_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU4502554.1"
                     /translation="MLRASLDDPDLQFVAINDLTDAKTLAYLLKYDSVHGTLKGSVEA
                     KDDQIIVDGKAIKVLAVKDPKELPWKDLNVEVVIESTGRFTDREGAGKHLSAGAKQVI
                     ISAPAKDPDVTIVLGVNEDKFDPKTHHIVSNASCTTNCLAPVAKVLLETFGIKHGVMT
                     TIHSYTNDQQLLDLPHKDLRRARAAGMSMIPTSTGAAKALHLVLPQLKGKMDGLAIRV
                     PTPNVSLVDLTVETEKDCDVAAVNEVFRKASNGALKGILQYSEDPIVSIDQKGDDHSA
                     TVDAPLTTVIDKRLVKVIAWYDNEWGYSCRVRDLIKVLAAKSKGR"
     gene            1029..2222
                     /locus_tag="VFR82_12960"
     CDS             1029..2222
                     /locus_tag="VFR82_12960"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU4502555.1"
                     /translation="MHKQTIDDMSLAGKRVIIRADFNVPLDDSLQITDDTRIRSTLPT
                     INRVVDDGGKAILCSHLGRPKGKFDPKFSLAPIAKRLGRLLGKDVIFAPDCIGPNIEN
                     LVAKMKVGDVMLLENLRFHAGEEQNDEAFSKALASLGDVYINDAFGAAHRAHASTVGI
                     TKYVPMAGAGFLLKKEIEYLEGAVENPVRPFVAILGGAKVSGKIGVIENLGKRVDKVI
                     IGGGMAFTFLKAKGLEIGNSLVENDMLDFAKGIEEHAFSRGVKFYLPVDCVVAASREP
                     GAETKIVPVQEIPKGWYALDIGPASVKLFTEAVQNAKTILWNGPMGVFEIDAYARGTL
                     SIAHAIADAYALTIVGGGETAMAIHRAGESENISFISTGGGAALELLEGKPLPGLVAL
                     PERKD"
     gene            2239..3012
                     /gene="tpiA"
                     /locus_tag="VFR82_12965"
     CDS             2239..3012
                     /gene="tpiA"
                     /locus_tag="VFR82_12965"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU4502556.1"
                     /translation="MRTTLIVGNWKMNKTASEAAGFVVELSRAIPNNPAVEIVLAPPF
                     TALASVRTTLDPASNMALGAQNLHWEQQGAFTGEISGAMLRDLGCTYVLVGHSERRTL
                     FGEQDGDVHKKVRSALVNGLRPILCVGESLDQRTAGQTDSIVTGQLAAGLADLTSDDL
                     GRVTIAYEPVWAIGTGHAATTEQAAMVHRTIRAHIAAGWNQERAARMRILYGGSVTPQ
                     NAESLLGESEIDGALVGGACLKTESFATIARAAVNRRIG"
     gene            3021..3374
                     /gene="secG"
                     /locus_tag="VFR82_12970"
     CDS             3021..3374
                     /gene="secG"
                     /locus_tag="VFR82_12970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU4502557.1"
                     /translation="MHTLVIIIHIIACFLMIGAILLQSGKGAEIGAAFGGSSQTVFGS
                     RGPANFLSKFTVVVAAVFMLTSMGLAILAKEKTFSSTVIDLKKKDTSQEVPAAAPAQK
                     PDTDSHSSSDPATAH"
     gene            complement(3626..>3865)
                     /locus_tag="VFR82_12975"
     CDS             complement(3626..>3865)
                     /locus_tag="VFR82_12975"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247688.1"
                     /note="catalyzes the transamination of the branched-chain
                     amino acids to their respective alpha-keto acids; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HEU4502558.1"
                     /translation="LAREQGIVVVEERFTRDEMYIADEVFVTGTAAELTPVREIDNRR
                     IGTGKPGVVTAALQKRFFAIVRGEDPSHDSWLTRV"
BASE COUNT          868 a         1144 c         1069 g          784 t
ORIGIN      
        1 gtgctgcgtg cctcactcga cgatcccgat ttgcagttcg tcgccatcaa tgatctaacc
       61 gatgccaaga cgctggccta tttgctgaag tacgattccg tgcacggcac cctgaaggga
      121 agcgtcgagg ccaaggatga ccagatcatt gtcgacggca aagcaataaa ggtgctggcc
      181 gtcaaggatc caaaagaact gccctggaag gatttgaatg tcgaagtcgt catcgaatcg
      241 accggccgct ttaccgatcg cgagggagcg ggcaagcacc tatcggctgg cgccaagcaa
      301 gtcatcattt ccgcccccgc taaagatccg gatgtgacga tcgtcttggg agtcaatgaa
      361 gacaagttcg atcccaagac gcaccatatc gtgagcaatg cctcctgcac gaccaactgc
      421 ctcgcgccgg tggccaaagt cctgctcgag accttcggta ttaagcacgg ggtcatgacg
      481 acaatccatt cctacaccaa cgaccaacaa ttgctcgatc ttcctcacaa agatctacgg
      541 cgagctcgtg cagccggtat gtccatgatt cccacgagca ccggcgccgc aaaagcactc
      601 catctcgtgt tgccccaatt gaagggcaag atggacggtc tcgcgattcg cgttcccacc
      661 ccgaacgtct cattagtgga cctcaccgtg gaaaccgaaa aagactgcga tgtggcggca
      721 gtcaatgagg tgtttcgcaa ggcctcgaac ggcgccttga aaggcatcct gcagtattcc
      781 gaagatccga ttgtctcgat cgatcagaaa ggcgatgacc actcagccac cgtggatgcg
      841 ccgttgacca cggtcatcga taaacggctc gtcaaggtca tcgcctggta cgacaacgaa
      901 tggggttact cgtgccgggt tcgcgacctc attaaagtac tcgcggccaa gagcaagggc
      961 cgctaaaccg caactccccc actgcacgtc ggcctgcttc acgctgacca atagggatcg
     1021 ccatgaatct gcataagcaa acgatcgacg acatgtccct ggccggcaag cgggtcatca
     1081 tccgcgcaga ctttaatgtc ccgttggacg attccctcca gattaccgac gacacccgta
     1141 tccgttccac ccttccaacc atcaaccgcg tagttgacga cggcggcaaa gccattctgt
     1201 gttcgcacct cggccggccg aaaggcaagt tcgatccaaa gttcagcctc gcgcctattg
     1261 ccaaacggct gggaagactg ttgggaaagg acgtgatctt tgcgccggac tgcatcggtc
     1321 cgaacatcga aaatctcgtc gcgaagatga aagtgggcga cgtcatgctg ctcgagaatc
     1381 ttcgtttcca tgccggagaa gagcagaacg acgaagcctt ttccaaggcc ctcgcatcgc
     1441 tcggcgacgt gtatatcaat gatgcgtttg gagcggcaca ccgagcccat gcctccaccg
     1501 tcggcatcac gaaatatgtg ccgatggctg gggccggctt cctcttgaaa aaagaaattg
     1561 aatacctcga gggcgcagtg gagaatccgg tgcgtccgtt cgtcgccatt cttggcggcg
     1621 ccaaagtctc ggggaaaatc ggcgtcattg agaatctcgg caagcgggtg gacaaagtca
     1681 tcatcggcgg cggtatggca ttcacctttt tgaaagcgaa aggcctcgag atcgggaact
     1741 cactcgtgga aaatgacatg ctcgactttg ccaaaggcat tgaagagcat gccttctcgc
     1801 gcggcgtgaa attttatttg cctgttgact gcgttgtggc cgcgagccgc gaacccggtg
     1861 ctgaaacaaa gatcgttccc gtccaggaaa tcccgaaagg ctggtacgcc ctcgacatcg
     1921 gtcccgcatc ggtgaaactc tttacggagg cggtgcagaa cgccaagacc atactctgga
     1981 acggccctat gggcgttttt gaaatcgacg cctatgcacg cggcacgttg tccatcgcgc
     2041 atgccattgc tgatgcctat gccttgacga ttgtcggcgg cggcgaaacc gcaatggcga
     2101 tccatcgagc cggggaatcg gaaaatatct cgttcatctc gacgggagga ggcgcggcac
     2161 tggaattact ggaaggcaaa ccattgccgg gcttggtggc cctgcccgag cgcaaagact
     2221 agcggcggca cagctaccgt gcggactacc ctcatcgttg gcaactggaa aatgaacaaa
     2281 acggcctcgg aggccgcggg cttcgtcgtc gaattgtccc gtgccattcc caataatccc
     2341 gccgttgaaa tcgtgcttgc cccgcccttc accgcattgg cgtccgtgcg tacgacgctg
     2401 gacccggcat ccaacatggc tcttggcgcc cagaatctcc actgggaaca gcagggcgcc
     2461 ttcacaggtg aaatttccgg agccatgttg cgcgacctgg ggtgcacgta tgtgctcgtg
     2521 gggcactccg agcgacgaac cctcttcggc gagcaggacg gtgatgttca caagaaagtc
     2581 cgatcggcat tggtcaacgg acttcgtccg attctctgcg tcggtgaatc cctcgaccaa
     2641 cggacagccg ggcaaacgga ctcgatcgtg actgggcaac tggcggcagg actcgcagat
     2701 ctgacatccg acgatcttgg acgtgtcacc atcgcctatg aacccgtgtg ggccatcggc
     2761 acgggacatg ccgccacaac agagcaggcc gcgatggtcc accggactat tcgcgcgcac
     2821 attgcagccg gctggaacca ggagagggcg gcacgcatga ggattttgta tgggggaagc
     2881 gtgacaccgc agaatgcgga atctctgctg ggggaatcgg agatcgatgg cgcacttgtc
     2941 ggcggggctt gtctcaaaac agagtcattt gctacaatcg cccgcgctgc tgtgaaccga
     3001 cggattgggt gagagggtct atgcatactt tggttatcat cattcacatc atcgcctgct
     3061 tcctcatgat cggggcgatt ctccttcaat cgggaaaagg agccgaaatc ggcgcggcct
     3121 tcggcggatc gagccagacg gtattcggta gccgcggccc cgcgaatttt ctcagtaagt
     3181 tcacggtcgt ggtcgcagcg gtcttcatgt tgacctcgat gggacttgcg attcttgcga
     3241 aagaaaagac gttctcgtcc accgtcatcg atctcaagaa gaaagacacc tctcaagaag
     3301 tccccgctgc tgctcctgct caaaaaccgg acactgactc gcactcctcg agcgatcccg
     3361 ccaccgccca ctaatccgtc gtctcaagct ttcgagcgta gatgtaccct tttttctcac
     3421 gcccgacccg cggcgattgt tttcacccgc taaccaaccg agtccttcaa tcaatgtccc
     3481 aggaccaatg tcccaggacg cgcctatccg cagattcaga cacatcggat gcagaggcct
     3541 agagcctctc agtgacggca acgaaacccg gacaattagg tgtggctaga cttgggcggg
     3601 tatcaggaag tatttcagca gctcgttaga cacgggtgag ccaggaatca tgcgagggat
     3661 cttctccccg cacgatcgcg aaaaaccgtt tttgcaatgc ggccgtcacg acgcccggct
     3721 tcccggttcc gatccggcga ttgtcgattt ctctgactgg agtcagttcg gcagccgtcc
     3781 ccgtcacaaa cacttcgtcg gcgatgtaca tttcatcgcg cgtgaaccgc tcttcgacca
     3841 cgacgatgcc ctgctcccgt gctag
//
LOCUS       DASTQY010000400         5705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_288804, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000400 DASTQY010000000
VERSION     DASTQY010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288804"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..514
                     /locus_tag="VFR82_12980"
     CDS             209..514
                     /locus_tag="VFR82_12980"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEU4502559.1"
                     /translation="MQTYVSLVKFTQHGLQTMKDKGIERAEAVKHNAQTLGGKLIQAY
                     YCLGEYDVIAIWEFPDNKTAMKAAVLNASLGHIEITTMPAVDREEWKSLLQQTIGKK"
     gene            760..1437
                     /locus_tag="VFR82_12985"
     CDS             760..1437
                     /locus_tag="VFR82_12985"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU4502560.1"
                     /translation="MITVGLAAVLGLSLLWTGPAPAQQGHQHERMPNVMEYLDHLDRP
                     ERDQDQKPAQVVEALAIKPGMHVADLGAGSGYFTRRFVEAVAETGKVYVIDIEPEALK
                     YVEDSLVHMQRRFDTEFILARPDDPKIPFESVDIIFVCNTYHHLEDRTEYFRNVRSSL
                     KPAGRLVVIDFYHDDRSGELSFPKRHLVAREKVVEEMTGAGYRLAREHTFLAKQYFLE
                     FVGAQDR"
     gene            1645..2112
                     /locus_tag="VFR82_12990"
     CDS             1645..2112
                     /locus_tag="VFR82_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502561.1"
                     /translation="MYVELRRQVTTQREAFSTSDIVSPARSAYLISTFDLTLLGSFVS
                     AGGILFLCRGKQPQFGSRWFTAERSIILSEEGREQHERATLLEAARWLTVGALILLIA
                     FLHGAEEGYLFGPWSDVMFHVVFVSTCWATAALRINRTVAVTLPASPAPQVRN"
     gene            2230..4218
                     /locus_tag="VFR82_12995"
     CDS             2230..4218
                     /locus_tag="VFR82_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEU4502562.1"
                     /translation="MKDDQPVVPATVTLPEITVKAVVLSVVLAAVLAAANAYLGLFAG
                     MTVSASIPAAVASMAILRLFKRSNILENNIIQTAASSGEALAAGVIFTIPALLLVGYW
                     SDFDYWQTASIATVGGLLGVLFTIPLRRALIVTARLRFPEGVATAEVLKVAAPDRRET
                     GQAQAAGDFRTLLFGALLGGAVKFGESGLRLWAESLEGAAQVGRTVVYAGLNLSPALL
                     AVGCIIGLNTAFVVFLGGLLGWLFLMPAYGLINGLPPGRTSLAAAMTIWSGQIRYVGI
                     GAMLIGGLWTLTKLREPVWKSFETLRDGYRQAGSSQSGRAVPRTEQDASLAWIVLPFV
                     VSLVPMAWIYISVVNSVPIGLAMTILMAVAAFLFSSVAAYMAGLVGSSSNPVSGVTIA
                     TIMVASLLLVLFMGTGHPAGPAATLVIGAVVCCAAAMGGDNLQDLKTGHLVGSTPWKQ
                     QVMQVVGVLTGAAVLVPVLSLLQAKYGIGEPTIGHPHPLSAPQATLMASLTRSVFGAG
                     LPWPLVGVGAVIGVLVIFLDRRQEARRSDFRLPVLAVALGIYLPLKLSATIFLGGVIA
                     TLASRAAAKSTELSRRGILYSAGLITGEALLGILLAFPIALGALWPGLSPDPFMLFET
                     PPFGGWPGLVIMTLVGGLLYRIAAGPSKHKKRNDVLNY"
     gene            4332..4505
                     /locus_tag="VFR82_13000"
     CDS             4332..4505
                     /locus_tag="VFR82_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502563.1"
                     /translation="MFEGLMGSLLSLVIVGAVILGFTTFVATLLIWIFRTQDDVVEAS
                     AEPSIEVRLRRAA"
     gene            complement(4581..>5705)
                     /locus_tag="VFR82_13005"
     CDS             complement(4581..>5705)
                     /locus_tag="VFR82_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502564.1"
                     /translation="ATELNLAEQRERKRIAAEMHDHLAQMLVLVRLKLGQAKQGPAHR
                     SLEMIKQAEDVVNDSLTYTRNLVAELSPPVLHEFGLFAALRWLGEQMQRYQLQVTVQI
                     ESTGGLRLPEDQAVLMFQSVRELLMNAVKHAVAKQAFVLVEEREGYLQIVVEDQGVGF
                     DLLAMATQQPSPLSSKFGLFSIRERMNAMGGRLELHSAPGEGTRATLILPCSSRQQPS
                     LEPASKVFLGTAASISQSRFHGPCRVLIADDHTMVREGLRSVLEGFPDIDVVGEASNG
                     QEAIDFTERFKPAAIVMDINMPLINGIEATARIKTRHPEIVVIGMSVNASADNHDAMR
                     TAGASMLLTKEAAVDQLHGAIQQAVLEIGQPGRDALGRSR"
BASE COUNT         1106 a         1720 c         1648 g         1231 t
ORIGIN      
        1 aaaacccctt gtctgcgtga gcggatgcct ccaaggaacg cacctcatat aataaaatgg
       61 tcctaaccct gctctttctt ctcactccgg gtgatggagg ccatgcgttc acctctcgct
      121 tgagcagcgg ggtctatcat ccatccccta cattccccta caatttgttc gaacatcgca
      181 cttcacagct cacccgggag gacccactat gcagacctac gtcagcctcg tgaagttcac
      241 gcaacacggt ctccaaacga tgaaggacaa ggggatcgag cgagccgaag ccgtcaagca
      301 caatgcccaa accctcggcg gaaagctcat ccaggcgtat tactgtctcg gcgaatacga
      361 cgtcatcgcc atctgggaat ttccggacaa caagacggcc atgaaagccg cggtcctcaa
      421 tgcctcgctc gggcacattg aaatcacgac catgccggcc gtcgaccgcg aggaatggaa
      481 gagtctgctg cagcagacga tcggcaaaaa atagtacgga tgaggagctg ttggtctccc
      541 ttgctcgccc aacgcgcaca ccggattaat cgtattttca gaaagacggt gctcgttgaa
      601 gcgcgcagtg agagaccaat cagccctcat cctaggagaa gcacacgagc gagccttgaa
      661 aggagccttc atgcctgtac cgcaggtcgc agaaggacta aagggggtgc tcactgagga
      721 acatttatgc ggtctcgttg gaggaatgga tcaagccgga tgatcacggt gggcctggcg
      781 gctgtgcttg gactgagtct gctgtggacc ggaccggcgc cggcgcagca agggcatcag
      841 cacgagcgga tgcccaacgt gatggagtac ctcgatcacc tcgatcgtcc cgagcgcgat
      901 caggaccaga aaccggcgca ggtcgtcgag gcgttagcga tcaagccggg catgcatgtc
      961 gccgacctgg gcgcaggctc cggctacttc acgcggcgtt ttgtggaagc ggtcgctgag
     1021 accggcaaag tctacgtaat cgatatcgaa ccggaggcat tgaagtacgt ggaggacagc
     1081 ctcgtccaca tgcaacggcg gttcgatacg gaattcatct tggccaggcc ggacgatccg
     1141 aaaatcccat ttgaatccgt cgatattatc ttcgtctgca acacgtatca ccacctcgaa
     1201 gaccgcaccg aatacttccg caatgtcaga tcgtctctca aacccgccgg acgcctcgtc
     1261 gtcatcgact tctatcatga cgaccgttcg ggcgagctga gcttccccaa acgccatctg
     1321 gtagcacgtg agaaggtcgt agaggaaatg acgggagccg gctaccggct ggcaagagaa
     1381 cacaccttcc tcgcaaagca gtacttcttg gaatttgtcg gggctcagga ccgctgaatg
     1441 accaccgtgt atgcattgcc atgcatagga agtgtgtatt gtgttcgccc gctgtctgat
     1501 gtaggttacg ccccgctcgt ggacgagccg tgaggcgtat gtcgtaacct ttctctcatc
     1561 ccagcagctc gatctgagga ggatgttcaa taaagatctt ctggcaaggc gagaactagc
     1621 gaaggagcga ggcgtactgt cgtcatgtac gtcgagcttc gcagacaggt gacaacacag
     1681 cgagaagcct tttcaacatc cgacatcgtg tcgcccgcaa ggagcgccta cttgatcagc
     1741 acattcgatc tgactctgct tggcagcttc gtgtcggccg gcggcatttt atttctctgc
     1801 cgaggaaagc agccgcagtt cggctcacgc tggttcaccg ccgagcggtc aatcatcctg
     1861 tcagaagaag gccgtgagca gcacgagcgg gcaaccttgc tcgaagctgc ccgatggctg
     1921 acagtcggtg ccttgatcct cttgatcgcc ttccttcacg gagcagaaga aggctatctc
     1981 ttcggaccgt ggagtgacgt catgttccac gtcgtcttcg tctcgacctg ctgggccacg
     2041 gcggcgcttc gtatcaatag aacggtggcc gtcaccctgc ccgccagtcc ggcacctcaa
     2101 gttcgcaact aactgcaacg tgtattgcca ccgttgcctt tttccaaggc tgcgttatga
     2161 tgcgcccgcc ctcatctttc cgtaacgttc ctacactatt tcaactgtca ccctggttcg
     2221 gtggcgctca tgaaagatga ccaaccggtc gtgccggcaa ctgtcacgct ccctgaaatt
     2281 accgtcaaag cggtcgtcct gtccgtggtc ctcgcggccg tcctggccgc tgccaatgcc
     2341 tatctcggtt tgttcgccgg catgaccgtg tccgcttcga ttcctgccgc cgtcgcctcg
     2401 atggcaattc ttcgtctgtt caaacggtcc aatatcctcg agaacaacat catccagacc
     2461 gccgcctcat caggggaagc actggccgcc ggcgtgatct tcacaatccc ggccctgctg
     2521 ctcgtcggct attggtccga cttcgattat tggcagaccg cctcgattgc cacggtcggt
     2581 ggtctcctcg gcgtgctctt taccattccg ttgcggcggg cgctcattgt caccgcgcgc
     2641 ctgcgttttc ccgaaggggt ggcgacagca gaagtcttga aggttgcggc accggatcga
     2701 cgagagacag gtcaggccca agcggccggc gatttccgga cgttgctctt cggcgccctg
     2761 cttggcggcg cggtcaaatt cggtgaaagc ggcttgcgcc tctgggcgga atccctggaa
     2821 ggtgcagcac aggttggacg caccgtcgtc tatgcaggac tcaatctctc gcccgcgctg
     2881 ctcgccgtgg gatgtatcat cggcttgaat acggccttcg tcgtgttcct cggcggcctg
     2941 cttggctggt tatttctgat gccggcttac ggactcatca atggcctgcc gcccggtcgg
     3001 accagcctcg ccgcggcgat gacgatctgg agcggacaga tccgctacgt cggcatcggg
     3061 gcgatgctga ttgggggact ctggacattg acgaaattgc gtgagccggt gtggaaaagt
     3121 ttcgagacgc tgagggatgg ttatcgacaa gccgggtcat cgcaatctgg acgagccgtg
     3181 cccagaacgg agcaggatgc ttcgctcgct tggattgtcc tcccctttgt cgtgtcgttg
     3241 gtgccgatgg cctggatcta tatcagcgtc gtgaacagcg ttccgatcgg ccttgcgatg
     3301 acgatcctga tggcagtcgc cgcctttctg ttctcctcgg tcgccgccta catggccggt
     3361 ctggtcggca gctccagcaa tccggtgtcg ggcgtcacga tcgccacgat catggtagcg
     3421 tcgctcttgc tcgtgctatt catgggaacc ggtcatccgg ccgggccagc ggcgacgctt
     3481 gtgatcggcg cggtggtctg ttgcgctgcg gccatgggag gcgacaactt gcaagatcta
     3541 aaaaccggtc atttggtcgg atcaacgccc tggaagcagc aagtcatgca agttgttggc
     3601 gtgctaactg gcgccgccgt gttggtgccc gtcctctcgc tgcttcaggc caagtacgga
     3661 atcggcgaac cgacgatcgg acatcctcat ccgttgagcg cgccccaggc gacgctgatg
     3721 gccagtctca cgcgcagcgt cttcggggcg ggcctgccct ggccgctcgt cggagtgggc
     3781 gccgtcatcg gcgtgctcgt catttttttg gatcgcagac aggaagcgcg ccgcagcgac
     3841 ttccggctcc ctgtcctcgc cgtggcgctt ggcatctatc tgccgctgaa actctcggcc
     3901 acgattttcc taggtggcgt gatcgcaacc ttggcaagca gagcagccgc gaaaagcact
     3961 gagctgtcac gccgcgggat cctctactct gccggcttga tcacgggtga agcactgttg
     4021 ggcattctgt tggccttccc aattgccctg ggcgccctgt ggcctggcct tagtccagat
     4081 cccttcatgt tgtttgagac gcctcccttc ggcggctggc ccggcctcgt gatcatgacg
     4141 ttggtcggag ggctgctgta tcggatcgca gcagggcctt cgaagcacaa aaaaaggaac
     4201 gatgtcctga actattgagc ctcgccaatc ttggcaccct aggaaacgcc tagggaagct
     4261 cttgctgcca ctgtcggcct ttgtttgtag aatactgtag acctcaacac aaccttctca
     4321 agaggtgcct tatgtttgaa gggttgatgg gaagcctgtt aagcctcgta atcgtcggcg
     4381 cggttattct cggcttcacc accttcgtgg cgacgctgct catatggatt ttcaggacac
     4441 aggacgatgt ggttgaagcg tctgccgagc cgtcgatcga agtgcggctg cgcagggcgg
     4501 cataaccggc aggcctgagg ccggcgccaa ttccgagact tgcgggtgaa ggtcgtcgtg
     4561 gcgaacaggg accgaggccc ctaacgggac cggcctaatg cgtctcttcc aggctgccca
     4621 atttccagaa cggcctgttg gatcgcgcca tggagctggt cgacagccgc ctccttggtc
     4681 aagagcatcg aggcgccggc cgttcgcatg gcatcgtgat tatcagccga ggcgttgacc
     4741 gacataccga tgaccacgat ctccggatgc ctggtcttta ttctggcggt ggcttcgatg
     4801 ccgttaatca gcggcatgtt gatatccatg acaatcgccg ccggtttgaa tcgctcggtg
     4861 aagtcaatgg cttcttgtcc attggacgct tcaccgacca cgtcgatgtc gggaaaacct
     4921 tcgagcacgg aacgtaatcc ctcccgcacc atcgtgtgat cgtcggcgat cagaacgcga
     4981 caggggccat ggaatcgact ctgacttatc gaggcggctg tcccgagaaa caccttcgac
     5041 gcgggttcaa gactgggctg ctgtctgcta gagcacggaa ggatgagcgt ggccctcgtg
     5101 ccttctcccg gcgcagaatg caattccaag cggccgccca tcgcgttcat gcgttcgcgg
     5161 atgctgaaca aaccgaactt cgacgaaaga ggcgacggct gttgcgtcgc catggccagc
     5221 aaatcaaacc ccaccccctg gtcttcgacg acaatttgta aatatccctc ccgctcctcg
     5281 acgagcacaa aggcctgttt ggcgaccgcg tgcttgaccg cgttcatgag cagctcgcgc
     5341 accgactgga acatgagcac ggcctggtct tccgggagcc ggaggcctcc cgtcgactcg
     5401 atctgcacgg tgacctgcaa ctgatagcgt tgcatctgct caccgagcca ccggagcgcg
     5461 gcgaataacc caaattcatg caacaccggc ggactgagtt ccgcaacgag atttctcgtg
     5521 taggtgagcg agtcgttgac gacgtcttcc gcctgcttga tcatctccag cgagcgatga
     5581 gcgggccctt gtttcgcctg acccagcttc aaccgcacga gcaccaacat ctgcgccaag
     5641 tggtcgtgca tttcggcagc gatccgtttt cgctcccgct gctccgccag gttcaactcc
     5701 gtcgc
//
LOCUS       DASTQY010000401         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_906146, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000401 DASTQY010000000
VERSION     DASTQY010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906146"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VFR82_13010"
     CDS             complement(<1..1031)
                     /locus_tag="VFR82_13010"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4502565.1"
                     /translation="MSTFAQMSLPPFLAQRLLQSGITSPTPIQQAAIPPVLEGRDVMA
                     QAKTGSGKTLAFLLPMIERALRPSMTSQGASVASVGGGKRGPQFLILAPTRELALQIE
                     TELRKYAPPAVTSLAVYGGTPIERHYRALRHLPKVVVGTPGRLLDLAGSGHLYLGAVT
                     WLVMDEADQMLDRGFLRDIQRILALLPPGRQTLLFSATFSPEIQQLAGSMQRNPARIS
                     VDPGISSPTTITHAYYVVPAEQSRTQLVHTLLQTLKGGERSMIFCDQKYKVRRLAAKL
                     GGETASVGAITGNHSQAQRERTLGAFRAGRLTSLVATDVAARGLDIPDVAQVIHYELP
                     VNPNSYVHRA"
     gene            complement(1177..1476)
                     /locus_tag="VFR82_13015"
     CDS             complement(1177..1476)
                     /locus_tag="VFR82_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU4502566.1"
                     /translation="MGSKIYVGGLPYSTTEQQLSDLFAAHGSVASARIITDKFTGQSR
                     GFGFVEMSGDSEAQAAIAALNGTQLGGRTLTVNEARPQEPRSGGGGRGGFGGGRH"
     gene            complement(1721..>2473)
                     /locus_tag="VFR82_13020"
     CDS             complement(1721..>2473)
                     /locus_tag="VFR82_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502567.1"
                     /translation="YLLLAVGWMLVQDGVAPVEVSAIPVENQAEKQANFKQQALGGLF
                     HRWTFDQQKPGDTLSGFSQFIVGSNGSPVWTVSDEADAPSPPNILKAESSCQTGSCYQ
                     LLVADQLHYEYPDVTVRLRFPAEGGIGQGGIVIGLQDERHFYAAVVDLAAKKVEVVRV
                     LDDQVAVLGETAITPKAIEWHSLRMNRDTIISKDVIEVFFDGRLLLSVQDQTLGLGQV
                     GLLVRGTSPMYFDSLHAVPLFSQRPVSSPAAY"
BASE COUNT          544 a          737 c          703 g          489 t
ORIGIN      
        1 gcacgatgca cgtacgagtt tggattgacc ggcagttcgt agtgaatgac ctgcgccacg
       61 tccgggatat ccagtccccg agccgccaca tctgtggcca ccagtgatgt cagccgtccc
      121 gctcgaaagg caccaagcgt ccgctcgcgc tgggcttggc tgtgattgcc ggtaatcgcg
      181 cccaccgatg cggtctcccc gccaagcttg gcggccaaac gccgaacttt gtatttctga
      241 tcacagaaaa tcatcgatcg ctctccgccc ttcagcgtct gtagcaaggt atggacgagt
      301 tgtgttcgcg attgctcggc cggcaccaca taatatgcat gggtaatggt ggtcggactg
      361 ctgattccgg gatcgaccga aatcctggcg ggattccgct gcatcgagcc ggcaagttgc
      421 tgaatctccg gtgaaaaggt ggcagagaac aacaaggtct gccgcccggg cggcaataaa
      481 gccaggatgc gctgaatgtc acggagaaag ccgcgatcca acatctgatc cgcctcgtcc
      541 atgacgagcc atgtcaccgc gccaaggtaa aggtggccgg atccagccag gtcgagcagc
      601 ctgcccggcg tgccgaccac gaccttagga aggtgccgaa gcgcgcggta atgccgctcg
      661 atcggagtcc cgccatacac agctagggag gtgaccgccg gcggcgcata ttttcgtaac
      721 tccgtctcaa tctgaagcgc cagctctctc gtcggagcca gaattaagaa ctgcggaccg
      781 cgttttccac ctccgactga ggcgacggac gccccttggc ttgtcatgct gggccgcaag
      841 gcgcgctcga tcatcggcag gagaaaggcc agtgtttttc cgctccctgt cttggcctgc
      901 gccatgacat cgcgtccttc aagcacaggc ggaatcgctg cctgctggat cggcgtgggg
      961 gaggtgatcc cggattgcag taatcgctgg gcaagaaacg ggggtagact catctgagca
     1021 aacgtcgaca tagaactcct tatgtaaatt agcttcctgg gactgctagg cggccgatca
     1081 ttcggccgag aaacgcatct gatagatggg caattctagg aaggatatga gcggggctgc
     1141 cggtgaggca gccccgacat gaaaaacggc tcgcactcag tgccggccgc caccgaaccc
     1201 gccgcggcct ccgccgccag agcgtggttc ttgcggacgc gcttcgttga ctgtcaacgt
     1261 gcggccgcca agttgggtac cgttcaacgc ggcgatcgcc gcttgagctt ccgaatcccc
     1321 ggacatttcc acgaagccga agcctcgcga ctggccggtg aacttatcgg tgatgattcg
     1381 ggctgacgcc accgacccat gcgcagcgaa cagatcgctc agttgctgct cggttgtgga
     1441 atacggcaaa cccccaacat aaatcttcga acccattggg ttcctccttt gagaaaattg
     1501 gtgttgtctt ggactcgaga agaggtgagg aaggaatggg ccgaagacgc aaacacgatg
     1561 gcgactcagg tctggcttcc gatcagatcc ccgggcagaa ccaaaacaac atcagtatca
     1621 agccttgtca ctgttctact gcatcaccac caggcctttc gcggtgatac accgatgcac
     1681 tgtaacgtag gagcggttca aatgcaagcc cagactggtt tcaatacgct gcagggctcg
     1741 acaccggacg ctgagaaaac agcggcacgg catgcaagct atcgaaatac atgggagaag
     1801 ttccacgcac gagcagtccg acttgcccaa ggcccagcgt ctggtcctgc acggaaagca
     1861 agagccttcc atcgaagaac acctcgatca cgtctttgct gataatggtg tctcgattca
     1921 tgcgtagtga atgccactct atcgccttcg gagtgatggc cgtctccccg agcactgcga
     1981 cctggtcatc aaggacgcgg acgacttcca ccttcttcgc tgccaggtcg acaacggctg
     2041 cataaaaatg tcgctcgtcc tgcaacccga tgacaattcc accttgcccg atgccacctt
     2101 ctgctggaaa acgcaaccga accgtcacgt ccggatattc gtaatgcaat tgatcggcca
     2161 ccaatagttg gtagcaactt ccagtctgac aggacgactc tgccttgagg atgttgggtg
     2221 gagagggcgc gtccgcttcg tcggatacgg tccagaccgg cgagccgttg ctgccgacga
     2281 tgaactgaga aaacccactc agggtatcac cgggcttctg ctgatcgaac gtccagcggt
     2341 gaaacaatcc gccgagcgct tgttgtttga aattggcttg tttctcggcc tgattctcca
     2401 ctggaatggc ggagacctcc accggcgcca caccgtcctg cacgagcatc catccaaccg
     2461 ccaggagaag ata
//
LOCUS       DASTQY010000402         4526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1297483, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000402 DASTQY010000000
VERSION     DASTQY010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297483"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..922)
                     /locus_tag="VFR82_13025"
     CDS             complement(143..922)
                     /locus_tag="VFR82_13025"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU4502568.1"
                     /translation="MCASTDRKIGISSSLDWNLIVVNPAALLQNQLLAALPQADLARI
                     SDRIEAVQMPLGHVLYESGDQLQHVYFPTNSIVSLLYVMEDGASAEIAIVGSEGIVGI
                     SLFMGGETTPSRAIVQSAGQAFRVSSKLLKTEFHRAGPLQEILLRYTQALITQMAQTA
                     VCNRHHSVEQQLCRWLLMSLDRLGSAELSMTQKLIADMLGVRREGVTEAAGKLQKLGL
                     IEYSRGRIVVLDRAGVERHACECYQVVKKEYERLLPGAKSK"
     gene            1047..1454
                     /locus_tag="VFR82_13030"
     CDS             1047..1454
                     /locus_tag="VFR82_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502569.1"
                     /translation="MHSREAPMLDKTCNGVVRFVQARTNNEKARLINSLPAIARKGLE
                     EDLNQIGEAYRFDLTREIAYYFQRQKAGLSMWSWQGLGSYDEIGELLARVASLDEPIT
                     EELANKLFVLATHRSVASPEPSREDRERNYHFG"
     gene            1608..2420
                     /locus_tag="VFR82_13035"
     CDS             1608..2420
                     /locus_tag="VFR82_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502570.1"
                     /translation="MSKQLEQSPILQGVYLESTESARASRGDLYAVVDYPFAMVSDAF
                     TNPENWCEALILHLNIKYCHAEREDGRKVLSVAAGKKSEQPLSETHRLTFAYNVLSGT
                     DYMAVNLDANKGPLGTRNYHVALELIALEGEKSFLHMEYSYTDGFLARLAMRLYFATS
                     GSAKVGFTKVGGENGEPTQLVRGVRGALERNTMRYYLALEAFLSAAATPDSRRFEESL
                     ERWYTATERYALQLHEVDRDDYITMKRNEYLRQQAPRELQKMTGVASPGEQL"
     gene            complement(2450..4000)
                     /locus_tag="VFR82_13040"
     CDS             complement(2450..4000)
                     /locus_tag="VFR82_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502571.1"
                     /translation="MKRRSFLQAAGTAALLAGSGARSVGAAVPAHNWEGHDFGPGPPV
                     KDRLYQGPFPQYAPEAFIAGSEVVMATTPSREIVPNFGMGLTVYVSGDYWPPRTGSDS
                     LEAYCENLIKLPFAQKVYIRLNWRDIQKRPGSLAFPEGWKVTFDLARQYNKRVCFRVM
                     LENPDYPDPGMPEFLVGKVPYVRPRGEWNRGPEHIRKDYSMPRYDHPDYQAAFRELNG
                     LLAAELNGNPLVEYMDTMMYGFWGEGHTWPFEGNPFPDNRTAEETFVQMFEAQRDAWT
                     RTPLVTNTQPDWSMVGNAEVLDRTIRTNNWLRTDTIFIENEQIEALSNRPPWVAAVSE
                     VGMTKGDPGSLRVTEGVTYTQNIIQHVMDIGANYWSLWHWHDIASRNILGYYEKFPEH
                     IDEIARRIGYRVRPSWIWQTEKDGYPGLVIGFVNDGIACVPGVLRVSVSTEDGRVRAS
                     GCLDPGYPMTRGVRQALFMLPKGTPWEGLKLKAELEVKGQRYPVRWACHQALNEDGSL
                     TMRANLET"
     gene            complement(4012..>4526)
                     /locus_tag="VFR82_13045"
     CDS             complement(4012..>4526)
                     /locus_tag="VFR82_13045"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU4502572.1"
                     /translation="TLAKSPEFVGTTRTTCVATMLDAGHAENALPQTAKAAVNCRIFP
                     GVAVETVRDALGAAIADPEVKVTVRGKPEASPVSEMRPDVMAAITKAVHADYPGVMVV
                     PYLESGGTDGKIYRAAGIPTFASSGLFIKPSEMFAHGLNERLPVASFYRGLDHIHRLA
                     MELGGASPGR"
BASE COUNT          922 a         1409 c         1320 g          875 t
ORIGIN      
        1 agtaactgtc aggctgattc gagtcgatta gaaggcggcc ggggaaaccc ggccgctttt
       61 ttcgttgacg agagaggacg tgataaacgg cgaaaagacc tgaagagcag atatccctga
      121 attaggccca gccccgaaac cgctacttcg acttcgctcc cggaaggagc cgctcatact
      181 cttttttgac cacctggtag cactcgcagg cgtgcctctc gactccagcc cgatcgagca
      241 cgacgatgcg cccccggctg tactcgatca gtccgagctt ctgcaacttt ccagccgctt
      301 ccgtgacccc ctcgcgccgc acgcccagca tgtcggcgat caatttctgg gtcatcgata
      361 attcggcgga gccaaggcgg tcaaggctca tgagcagcca gcgacacagt tgctgctcga
      421 cggaatggtg gcggttgcag acggccgtct gagccatttg tgtaatcagc gcttgcgtat
      481 accgcagtaa gatttcctgc aacggcccgg cgcgatggaa ttccgttttg aggagcttgc
      541 tggacacgcg aaaggcctgg cccgcgctct gcacgatggc gcggctcgga gtcgtctcgc
      601 cgcccatgaa cagggaaatc ccgacgatgc cctcgctccc aacgatggcg atttctgcgg
      661 aggcaccgtc ctccatcaca taaagcaggg aaacgatcga gtttgtcgga aagtacacgt
      721 gctgtagctg gtcgcccgac tcgtacagca catggcccag aggcatctgg accgcttcga
      781 ttctatcgct gatgcgcgcc agatcggctt gcggcaacgc ggcaagcaat tgattctgta
      841 gaagcgcggc gggattgacc acgatcagat tccaatccag gctggatgat attccgattt
      901 ttctatccgt gctagcgcac atagcgtccg taaagctccc tgggaaaggc acgagaaaag
      961 tggcccataa gtgcgctgcc gtacacatca cggaccgcgt gaggcgtact ttctaactcc
     1021 aaaacctgaa aaaccgggag agcgccatgc actcacgcga agctcccatg ctggacaaga
     1081 cctgcaacgg cgtcgtgcgg tttgttcaag cgaggacaaa caacgaaaag gcgcgcctga
     1141 tcaactcatt gccagcaatt gcgcgcaagg gccttgagga agatctcaac cagatcgggg
     1201 aggcctacag gttcgaccta acccgggaaa tcgcgtacta cttccagcga cagaaagcgg
     1261 gcctgagcat gtggagttgg caaggcctgg ggtcatatga cgagatcggt gaattacttg
     1321 ctcgtgtggc atcgctggac gagccgatta ccgaggaact tgccaataaa cttttcgtgc
     1381 tggcgactca cagaagtgtc gcaagcccgg aaccatcacg cgaggatcgc gaacgcaact
     1441 accacttcgg gtgaacgacc atcgcggctc aagaggttca atccaggcac tccattcgat
     1501 ggatcctcgt cgtggcctca gcggtatgcg ccgcctccgc gttcgccgaa gattccgcaa
     1561 ggccggaatc aagggccgca gtgtccttat ggaaccggca tgtcgctttg agcaagcagc
     1621 tcgagcaaag cccgattctg cagggcgtct acctggagtc caccgaaagc gcgcgcgcct
     1681 cgcggggaga tctctacgcc gtcgtcgact acccgttcgc catggtcagc gacgcattca
     1741 caaacccgga gaactggtgc gaggcgttga tcctgcacct gaacataaag tactgccacg
     1801 ccgaacgaga ggacggacga aaggtgctat cggtggccgc tggcaagaag agcgaacagc
     1861 cactgagcga aacccatcgg ctcacgtttg catacaacgt gctgtcaggc accgactaca
     1921 tggcggtcaa cctcgatgcc aacaagggac ctctaggcac ccggaactac cacgtcgcgc
     1981 tggaactcat cgccctggag ggcgagaagt cgttcctcca catggagtat tcatataccg
     2041 acggtttcct ggcgcgcctc gcaatgcgcc tctatttcgc gacgagcggc agcgccaaag
     2101 tcggtttcac caaggtcggc ggtgagaatg gtgagccaac acaacttgtt cgcggcgtgc
     2161 gcggtgcgtt ggagcgcaat accatgcgct actacctcgc cctcgaggca tttctgagcg
     2221 cagccgcgac tccggactct cggcgttttg aagaaagtct cgagcgctgg tacacagcca
     2281 cggagcgcta cgctctccag ttgcatgaag ttgaccgcga cgactacatc acgatgaagc
     2341 gcaacgaata cctgcgccaa caggcccccc gtgagctaca aaagatgacc ggcgttgcct
     2401 cgcccggtga gcagttgtag taacacacag cggaccgcat ccgccccctt cacgtctcca
     2461 ggttcgcgcg catcgtcaat gagccgtcct cgttgagcgc ctgatgacaa gcccagcgca
     2521 ctggatagcg ctggcccttc acttccagct cggccttgag cttcagccct tcccagggcg
     2581 tccccttggg cagcatgaac aatgcctgcc gcacgccgcg cgtcatcgga tagccaggat
     2641 cgaggcaacc gcttgcacgg acacggccat cctccgttga cacgctgacg cgaaggacgc
     2701 ccggaacgca agcaattcca tcgttgacga agccgattac caggccggga taaccgtcct
     2761 tctcggtctg ccagatccac gacgggcgca cgcggtagcc gatgcgtcgg gcgatctcgt
     2821 cgatatgctc cggaaacttt tcgtagtagc cgaggatgtt cctggatgcg atgtcgtgcc
     2881 aatgccataa ggaccagtaa ttcgcgccga tgtccatgac gtgttggatg atgttctgcg
     2941 tgtacgtcac accttccgtc acgcgcagcg accctggatc gcccttggtc atgccgactt
     3001 cgctcacggc cgcgacccac ggcggtcgat tgctcaatgc ctcgatctgc tcgttctcga
     3061 taaagatcgt gtcggtgcgc aaccagttgt tcgtgcggat ggtccgatcg agcacctcgg
     3121 cattgcccac catgctccaa tcgggctggg tattcgttac caacggcgtc cgggtccagg
     3181 cgtctcgctg agcctcgaac atctgcacga acgtttcctc ggcggtacgg ttgtcgggga
     3241 acggattgcc ttcgaaaggc caggtgtgac cctcgcccca gaacccgtac atcatcgtgt
     3301 ccatatactc cacgagcgga ttgccattca gctcggccgc tagcaagcca ttcagttcgc
     3361 gaaatgccgc ctgatagtcg ggatggtcat agcgcggcat gctgtagtcc ttccggatgt
     3421 gctccggccc ccgattccac tccccgcgtg gccggacgta agggactttg ccgaccagga
     3481 actccggcat gcccgggtcc gggtaatcgg ggttttcgag catgacgcga aagcagacgc
     3541 gcttgttgta ttgccttgcg aggtcgaacg tgactttcca tccctcgggg aaagccaggc
     3601 tgccgggacg cttctggatg tcgcgccagt tgaggcggat gtaaaccttc tgcgcaaatg
     3661 gaagcttgat caggttttcg cagtaggctt ccaacgagtc actaccggtt cggggcggcc
     3721 agtaatcgcc cgatacgtac acggtcagtc ccatgccgaa gttcggcacg atttcgcggg
     3781 agggggtcgt cgccatcacg acttcgctgc cggcgatgaa tgcctccggc gcgtactgcg
     3841 gaaacggccc ctggtatagc cgatccttta cgggtgggcc gggcccgaaa tcatgtccct
     3901 cccagttgtg ggcgggtaca gccgcgccaa cgctcctggc gccgcttccg gccagaagtg
     3961 ccgccgttcc cgctgcctgg agaaaagacc ttctcttcat aagcggccgc cttaccgccc
     4021 cggcgatgct ccgcccagct ccatggccag ccggtggatg tggtccagac cgcgatagaa
     4081 cgacgcgact ggcagtcgtt cgttgagtcc gtgcgcaaac atctcgctgg gcttgatgaa
     4141 cagaccggaa ctcgcgaagg tcggaatgcc cgccgcgcga taaatcttgc cgtccgtgcc
     4201 gccggattcc aggtagggca cgaccatcac gcccggatag tctgcatgca ccgccttcgt
     4261 gatggcggcc atcacatccg gtcgcatttc cgagaccggg ctggcctcgg gctttccgcg
     4321 cacggtgacc ttcacttctg ggtccgcgat ggccgcacca agcgcatcgc gcactgtttc
     4381 gacggcgacg cccggaaaga tacggcagtt gacggcagcc ttggccgtct gcggcaacgc
     4441 gttctccgcg tgaccggcat ccagcatcgt ggcaacgcac gtggtgcgcg tcgtgccgac
     4501 gaattccggt gacttcgcca gcgtag
//
LOCUS       DASTQY010000403         2776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1499538, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000403 DASTQY010000000
VERSION     DASTQY010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499538"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(351..2318)
                     /gene="pilQ"
                     /locus_tag="VFR82_13050"
     CDS             complement(351..2318)
                     /gene="pilQ"
                     /locus_tag="VFR82_13050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEU4502573.1"
                     /translation="MTHIQRHASTTVRILSALAAVSLPMAIPSTGASADVNAGVSMNR
                     PGIDQGASDRRLASAVTGIDVKKEADAVSVTISGDGSLLHEALRVDERRLVVDIPRVS
                     SEIHRPTLNVGHRLVKQIRIGDHSDKVRIVFDLGQQAEYAVEPQGASLLVRVHESIGG
                     GSMPEQDSTNAHTSAAPSAAADIPLIESAQGGRHGIQPVVHRTTPGFRVRPIQMMAEP
                     DAVEKHTPKDDLVVGETRYVGRRISLDFQQADISNVLRLIAEVSGFNIVIGDGVKSKV
                     TMKLVSVPWDQALDMILKMNGLGKIKQGNILWIDTLSNIAKQEDEEARAKDAKVKAED
                     LVDRVFYIRNLQATELMTSLRQNLSPRGLMQISTGTNALVVRDTETKMAVLKQLIDGL
                     DLEVPQVQIEARIVQADTVYARGLGVQWGIANADFGTNTFSAVGNLTGAFAPAAGTGA
                     SVIPRDFLVNLPAQVAGLPSVPGIGYSFGKLAPGFALDMRLSAGELLGLAKVIAAPKV
                     TTLDKREAKISQGESIPFQTTSLQGTQTTFVDANLELNVTPQITSRDPKEVGKQIMMK
                     VRATRNAVGARSNPAGPSIDRREANTQVIVRDGETMVIGGVFIDTQNNNVQGVPYLSR
                     IPVLGWLFKNKSESVSKQELLIFLTPSIVKA"
     gene            complement(2315..>2776)
                     /locus_tag="VFR82_13055"
     CDS             complement(2315..>2776)
                     /locus_tag="VFR82_13055"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEU4502574.1"
                     /translation="VPSPNDLAKESPQSALTLSPDLAAAPTDALPGAPPFPIGGYDPA
                     GRRDPFSPVLSQLAPGQTDPTLPPLQRIGLTDLNLIAIIWGAYGYTAMVQTPDGNGYT
                     VRKGTRVGPNSGVVSAVTEKGIIVQERFTDVYGRKQEREYVKLLHPKEGLE"
BASE COUNT          564 a          815 c          794 g          603 t
ORIGIN      
        1 gtcggcttga ggtagtgacg cgcgacatgg cgatcggtga caatgccaac cttgccggtg
       61 tccataagag gcttgagaag ggcgccgaga gaccgactca agcccggctc aacgacgatg
      121 tcatagctcc gttcgccgag acgaacaggg acatgttgac cgatcatgct tgtcatcgtg
      181 gcggtaggat gtgtcgtgta caagtaaacc ggggaatagt agcacaagcc tctgaaggat
      241 aatagctttc aagactgaga gaagagcagt caaacgggag gaggacaggc agccacagag
      301 agggagaaca cgaccctgca atcccctcgg tcagggatcg caggggcgcg tcaggccttg
      361 acgatggaag gagtcaggaa gatcaacagt tcttgtttcg acacgctttc cgacttgttc
      421 ttgaacaacc acccgagcac cggaatccgg gaaagatacg gtaccccctg aacgttgttg
      481 ttctgtgtat cgataaagac gccgccgatg accatcgttt cgccatctcg cacgatgact
      541 tgggtgttgg cctctcgacg atcgatgctc ggaccggccg gattgctgcg cgcgcccacc
      601 gcatttcgag ttgcgcgcac tttcatcatg atctgcttac cgacttcttt tggatcacgc
      661 gacgtaatct gaggagtgac gttcagttcc aagttggcat cgacgaaggt ggtctgcgtg
      721 ccttgaagtg aagtcgtttg gaacggaatc gattcgccct gcgaaatttt cgcttcccgc
      781 ttgtctagcg tggtcacttt cggcgcagcg atcaccttgg ccaacccgag caactctccg
      841 gcagagagcc tcatgtccag ggcaaaaccg ggcgccagtt tgccgaacga atagcctatg
      901 cccggcaccg acggaagacc agccacctgc gccggcaggt tcaccaggaa atcacgggga
      961 atcaccgacg cccccgttcc tgcagccggg gcgaatgcac ccgtgaggtt ccccactgcc
     1021 gagaacgtat tcgttccgaa atcggcattc gcaatgcccc attggacacc aaggccgcgc
     1081 gcgtagaccg tatcggcctg aacgatgcgt gcctcgattt ggacttgcgg cacctcgaga
     1141 tccaatccgt caatcagttg tttgagcacg gccatcttcg tttcagtatc gcgaaccacc
     1201 aacgcgttgg tgccggtgct gatctgcatg aggcccctgg ggctcaaatt ctgccgcaac
     1261 gaggtcatca actcggttgc ctggagattg cggatataga agacacgatc aacgaggtct
     1321 tccgctttga ccttggcatc cttcgctctc gcctcctcgt cctcttgctt ggcaatgttc
     1381 gacagcgtat cgatccagag gatgtttccc tgtttaattt ttccaaggcc gttcatcttg
     1441 agaatcatgt ccagtgcttg gtcccaggga acgctgacga gcttcatcgt gaccttgctt
     1501 ttgacgccgt caccgatcac gatgttgaag ccgctgacct ccgcgattaa cctcagaacg
     1561 ttactgatat cggcctgttg gaagtctaac gaaatacgac ggccgacata acgggtctcg
     1621 ccaacgacca ggtcgtcttt cggcgtatgt ttttccaccg catccggctc ggccatcatt
     1681 tgaatcggac ggactcgaaa tcccggcgtt gtccgatgca ccaccggctg tatcccgtgt
     1741 cgtccgccct gagcggactc gatcagagga atgtctgccg cggcggacgg agcagcactc
     1801 gtgtgagcat tcgtgctgtc ctgttcgggc atgctcccgc cgcctatgga ctcgtgcacc
     1861 cggacgagca ggcttgcccc ctgcggctcc acggcgtact ctgcttgctg acctagatca
     1921 aacacaatgc ggaccttgtc gctgtgatct ccaatacgaa tttgtttcac caggcggtgc
     1981 ccgacattca gagtgggtct gtggatctcg gaggagactc gtgggatatc cacgaccagt
     2041 cgacgctcgt ctactcggag cgcttcgtgc agtagtgaac catcaccgga gatagtcacg
     2101 ctgacggcat cggcctcttt tttgacatca atccctgtca cggcgctggc cagccgtctg
     2161 tcggaggctc cctggtcgat gccggggcgg ttcatggaca cgccggcgtt cacatccgcg
     2221 gaggcccccg tcgaggggat ggccataggc aaactgaccg cggccaaggc agataggatt
     2281 cgaaccgtcg tcgacgcgtg tctctgtatg tgtgtcattc taggccctct ttgggatgaa
     2341 gaagcttgac gtactcgcgc tcctgttttc tgccatacac gtcggtaaac cgctcttgca
     2401 cgataatccc tttttcggta accgcgctga ccacgccgct attgggtccg accctggtgc
     2461 cctttcgaac cgtgtacccg tttccatcgg gtgtctgcac catcgcggtg tatccgtatg
     2521 cgccccagat aatcgcaatg agattcaaat ccgtcagccc aatgcgctgt aagggcggca
     2581 atgtgggatc ggtctgacct ggagcaagct ggctgaggac gggcgagaac ggatcccgac
     2641 gtccggccgg atcgtagccc ccgatcggaa aaggcggcgc gcccggcaat gcatccgtcg
     2701 gagcggcagc gagatcaggt gacagcgtca aggctgactg cggcgactcc ttggccagat
     2761 cattaggcga gggcac
//
LOCUS       DASTQY010000404        19508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1163308, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000404 DASTQY010000000
VERSION     DASTQY010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 19508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19508
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163308"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..394)
                     /locus_tag="VFR82_13060"
     CDS             complement(164..394)
                     /locus_tag="VFR82_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HEU4502575.1"
                     /translation="MITPGRYRHYKGHDYEVIGVARHSETEESFVVYRALYGERGLWV
                     RPAAMFLDTVTVDGRSVLRFTRLPVQPSEEDG"
     gene            435..1130
                     /locus_tag="VFR82_13065"
     CDS             435..1130
                     /locus_tag="VFR82_13065"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4502576.1"
                     /translation="MGKQTASLTFALLAAVGLAATASSCQQQTWESVMTAGQEAVRQG
                     NYAEAEQMFQVAVQKAETFGREDRRVAVSLSQLAQVYAGQGKHVEAEPAYLQALKIYQ
                     AVHGENHADVAATLNNLGVLHRMYGQYAEAEPLLSRSLAIKERLLGPNHPDVALSVAN
                     LGQLYVAQGQPAKAEPLYRRALSIKREALGMSHPEVAKTLEDLANVLRKLGRSPEAVA
                     LDLQAREIRAKRS"
     gene            1163..1405
                     /locus_tag="VFR82_13070"
     CDS             1163..1405
                     /locus_tag="VFR82_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502577.1"
                     /translation="MPSENQPLSPNWRMNGMVARFVGIVAIALGFMLGMYGLDEQGSR
                     WLHTALGLLVTGMLAQGYALYCSIKRMQQLHDRPKT"
     gene            1402..1833
                     /locus_tag="VFR82_13075"
     CDS             1402..1833
                     /locus_tag="VFR82_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502578.1"
                     /translation="MKRAGRTVPSLSISLLLVFAALAACGGPWRDGYFKKGVDRLTQD
                     EISEKLGPPHTAKTPALGGDTIWTYRVPYSDKDLDPMDLSGITNAANEAASLIGKGAQ
                     DGPKPTLYCYRYTLTFNEQKVLKRWKREECVPGTRDSLHAG"
     gene            1852..2670
                     /locus_tag="VFR82_13080"
     CDS             1852..2670
                     /locus_tag="VFR82_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502579.1"
                     /translation="MITWLPQVDSSIVMDSLKSLLLLLSVLIARTLIVRSISRNAALT
                     MEAKRRWVVSARNSMVFIFLLGLVVIWAHELQAFAVSLVALAAALVLATKELLLCWSG
                     AALRVGGKVYMVGDRIQIAGHRGVVLDHDIFATKLLEIGPGQASHLYTGRVTVFPNSL
                     LFTNPLIKENPNQEYGLYTLTVPLAAGEDWKQAEARLLEAGRAECASFMTEASRQMKL
                     LEQQNLLEAPSPEPRITIQLSDVGRIVLVLRFPAPDRGRSRIEQAILRRFLADA"
     gene            2791..3159
                     /locus_tag="VFR82_13085"
     CDS             2791..3159
                     /locus_tag="VFR82_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502580.1"
                     /translation="MRTMLTVAVAALWLTNVTVLAVADDAITGEMNQLKADIKAEKEA
                     MKADTKTKKAEMKRLKKEHKQNIKAKKREMREKKKANKDRMKAQKDAMKTQGQDLNAA
                     GQDMKPAIPDLPAPQAPGAQ"
     gene            3253..4029
                     /gene="xth"
                     /locus_tag="VFR82_13090"
     CDS             3253..4029
                     /gene="xth"
                     /locus_tag="VFR82_13090"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU4502581.1"
                     /translation="MKIATYNVNSLRRRLPVVLDWLAKEKPDVLCLQETKVQDSEFPL
                     MALLPSGYEISYRGMKSYNGVAVLTRTKPESVRAGLDDGGEPDEPRLLHVVVNGIAVI
                     NTYIPQGFEIDSPKYAYKLEWFKRLRAYFEKHLSPSLPAIWCGDMNVAPRAMDVHSPE
                     KHLKHVCYHEAAREAYASTVAWGFYDVFVRLYPERQQFTFWDYRAPSSLDANKGWRID
                     HILATAPLTDKCLRAEVDVEPRRCRDASDHTFLWAEFSLP"
     gene            complement(4717..4887)
                     /locus_tag="VFR82_13095"
     CDS             complement(4717..4887)
                     /locus_tag="VFR82_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502582.1"
                     /translation="MTCQKCKGLMIAESRPDYSTGGTVLRCINCGLVLDPLIEQNRLA
                     RTRATHATMHAA"
     gene            5500..6540
                     /gene="ppk2"
                     /locus_tag="VFR82_13100"
     CDS             5500..6540
                     /gene="ppk2"
                     /locus_tag="VFR82_13100"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEU4502583.1"
                     /translation="MSDPDDATSPKAAKPEQPGTDQRVKANRDGRFLSDELEGIPTAV
                     PLEGDGYRKARKLAPVGVRGQGLTIEDLERINTRRGLIQLLQQKDVDLEAVRKILLYE
                     EELKCLQVEMVRLQRWVQSSGQRIAILVEGRDAAGKGGTIRRFTEHLNPRAMRVVALP
                     RPTEQERGQWYFQRYIQQLPNKGEIVFFDRSWYNRAVVEPVMGFCTKKEHQRFLQQVT
                     EFEHMLYEDGVMLIKFWFSISKEEQQQRFEARRNNPLKQWKLSPVDEKAQELWDSYTR
                     YKEEMFSKTHTTFSPWIIVKANDKQAARLESLRYVLNLLPYKGKEESKTRLAPDPNVV
                     TRYHRKMIELDF"
     gene            complement(6724..7608)
                     /locus_tag="VFR82_13105"
     CDS             complement(6724..7608)
                     /locus_tag="VFR82_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU4502584.1"
                     /translation="MKIVAGVDWSDAAFAAVEQLGLLYHPTDVTLVHGVDLGLFEYPI
                     VAEAANLQGYDIFRKSMFEAGCESLDRAATMLPNDIPKPRQLCEFRKPARFLLDTATA
                     MNADLIVVGTHDHSRVAELVLTSVSHRVLLHAMQPTLLVKGHAKPIRRVLVAIEGRDD
                     GRQICEWLSRHPFRTPVEATVLTAVPMLPTPMPELAPIPPFESWSEELMTQAQASVRV
                     CAEALLPSHYSASSRVMKGEPAPVISETAKEFDLVVVGSHGREGLDRFLLGSVSHAIV
                     HHVSCPVLVVRSGGPNPS"
     gene            complement(7641..8312)
                     /locus_tag="VFR82_13110"
     CDS             complement(7641..8312)
                     /locus_tag="VFR82_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU4502585.1"
                     /translation="MVVVFRDRAEAGRLLAEKLESYQGDPSGLILALPRGGVAVGYEL
                     SVSLHLALDVFVTRKLSTPDNPEYAIGALSETGAIYVNPEAVEGFHLSHSDLEAIIGN
                     ERQELARRQQLYRHGLPLAPIRDKTVILVDDGIATGATFFATIEAVQELSPRRLIAAI
                     PVAPLDSLALVRNRVDELIVIATPEPFVAVGHHYHNFTQVDDAQVLAYLKAARRAQGA
                     QAASG"
     gene            complement(8539..9045)
                     /locus_tag="VFR82_13115"
     CDS             complement(8539..9045)
                     /locus_tag="VFR82_13115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4502586.1"
                     /translation="MPTITLTKPFERHPSSVPNKTEAALRWRVLIVVPHTSIREMIRM
                     ILDGYADLIEVVGEASDAEAAVEQAKAMAVDLVLMDAHLANGVDATRQIRQILPQVVI
                     LGMSAEYTPYLYNAMIAAGAVAFVRMEDAADHLFRSIVFAMCTYGPMHRHVHPAAPSV
                     RHNAASCA"
     gene            9377..9643
                     /locus_tag="VFR82_13120"
     CDS             9377..9643
                     /locus_tag="VFR82_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502587.1"
                     /translation="MKISHVWKSGSAKKKTKPTYKIRWELLGLSRPDQAQSSLSMEAI
                     RSEVSLLATQGLSERTQSRSTTPAVGGRGLKRSITGSRRRGAGK"
     gene            10009..11370
                     /locus_tag="VFR82_13125"
     CDS             10009..11370
                     /locus_tag="VFR82_13125"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU4502588.1"
                     /translation="MPSGSPRRFIRPSFSKSLSPTIVIDKRSRTKFLIVGLLLLIAGC
                     SVLPGHWRPGWNEHWRDSKPVPDTAGSWKLTDLEVADGRFLALAISGGGSRAANFGAA
                     VMLELEQRKLLEQVDVISGVSGGTLPAAYYGLGDKAGAFTESTLRKTLGFDFQGSWIR
                     RWFLPQNIFRYWLSDFTRSDIMVQVFNNQLYHDATFQDLLPHPKILLNSTVHNDHTRF
                     TFTDERFAKLHSVLATYPVANAVNASSAFPGAFQDVTLQWYMEPPQYMHLYDGGPIDN
                     LGVQAITEYVNRNILGTSLDRLFPNGCLIVVIDATPASEHPELNTGQSSRSLIDYFVD
                     TNALDAMDAMLLESRRSLLAGMGIPIARQDQEMRGRLPVNDPRQCGCEVRHIALRQLM
                     YAGEESDGLAERVTQIKTKFWIGEDEQNDLFTAAKLLMKMVDDKKLLAEESLRTSCAV
                     ERK"
     gene            complement(11520..12092)
                     /locus_tag="VFR82_13130"
     CDS             complement(11520..12092)
                     /locus_tag="VFR82_13130"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEU4502589.1"
                     /translation="MAPRQLEGMRVAILVTDDFEQSELTEPKRALEAAGAKALIVSPA
                     SGEVQGMHHDEKADRFTIDQLLAEADPGQFDAALLPGGALNADALRVVPEAKSFITAI
                     DEAGKPIAVICHGPWLLVSAGLVSGRKMTSYHTIQDDLRNAGADWRDIEVLRDRNWVS
                     SRRPADLPAFNRELIALFADYQGSRTAKAA"
     gene            complement(12322..13455)
                     /locus_tag="VFR82_13135"
     CDS             complement(12322..13455)
                     /locus_tag="VFR82_13135"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEU4502590.1"
                     /translation="MSIFSLIVVFYLLTSTAQIWAGNQIYRYRDDSGTVTFTTELQSI
                     PEKYRQEAVPLATVPPPPPQRRHEPTLRIVTSSGEFRMGNYDTRTDATRMAIEAAKRD
                     ALEQVATYLESVTEVKNLDVTRDEIRTYTAGLVMVLNQQTSTRLENGVAVIHVDLTVQ
                     ADQQEVIQAITALRENENAKVQLASLRTETEQLRLQLDAANQALGTAMTAEQVQALTR
                     ERQQLLNQIQADALVARALTDYAYLTPMTVRQVNDLLLQARQLHPANQHLQAVEQALA
                     AKTDPSALPLVVPSHRNQPSASSDSTVSQRSPLTAPMLESPLAATLPPSPLATPLPPS
                     PLAVPLPPSPLAAPLPPSPLAKPLSPSPLAAPLAPSPLAAPLR"
     gene            complement(13611..14111)
                     /locus_tag="VFR82_13140"
     CDS             complement(13611..14111)
                     /locus_tag="VFR82_13140"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEU4502591.1"
                     /translation="MAKASRYTVTMVALSALIGSLGLFALSGHAADTRAISDGSKVTL
                     LYQITVPGDERFEVRDLSQFVQGQHQMLPAVEEAVTGMHRGEKTEVKLSEDQAFGAYD
                     SSKKTMVPKKELPAGAKPGDIIEDRKTGKQATITQMSETDAVMDYNHPLAGKPLLVTL
                     TILEVE"
     gene            14454..15086
                     /locus_tag="VFR82_13145"
     CDS             14454..15086
                     /locus_tag="VFR82_13145"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4502592.1"
                     /translation="MRITASLQSRISLFFISCLWLPGCAHLAYQHMNDPSRDAWQKPK
                     DVVERLDITPGSRVADLGAGGGYFTWHLASAAGPRGRVYAVDIDQTGLSIITKEMQTR
                     HITNVVPVQAEPTDAKLPEPVDLVFSCDTYHHLDDRVAYFKSLARYLKPDGRVAILDF
                     HPHGFFSGLLGHGSAAEEVRREMELAGYRRTADFDLIDRQHFQIFSRTGF"
     gene            15145..15879
                     /locus_tag="VFR82_13150"
     CDS             15145..15879
                     /locus_tag="VFR82_13150"
                     /EC_number="1.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004128 - cytochrome-b5 reductase
                     activity, acting on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-cytochrome b5 reductase"
                     /protein_id="HEU4502593.1"
                     /translation="MDSQTITRIKTKLPAPYQLTAINQDTHDTKTFCFALPEDATLDM
                     LAGDHLYVHATINGKAVKRPYTPSSVPGTTGYFDLTVKRYENGSVSRYLHDREIGDSV
                     LMSGPNSGGHWVDGMAKRAGFVAGGTGITPMISIIRWILAKGLPVELSLVFANKSEAD
                     IIFRDEWEANGREHANFHAYHILEQPPAGWAQGRGRITEETLRMHLPEPGPETVVFLC
                     GPPMMVDALEGTLKGMGYPEQSIILP"
     gene            complement(15969..16802)
                     /locus_tag="VFR82_13155"
     CDS             complement(15969..16802)
                     /locus_tag="VFR82_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502594.1"
                     /translation="MRNAFRFGECCVCILIAVATLYSHSVSAASLKEATFYARDHRFD
                     GPETISAGQTTIRLRNRGKEPHQLQFLKLDAGKTPAELAVALQSGSQSIPPWAKQVGG
                     PNAVGAGQAAEATLYLEPGSYVIICGIPGKTHQTHAALGMQKALRVIDTKSPPPDFRG
                     NFHMAMFEYEFVVVQPLRKGRHTFYVVNRGNQTHQASFIRLDPGASTEDVLTAIHQDS
                     PTRLPGTLIGGMSGLEPGRDGTFTADLTPGRYAIMCLFSNTVMSESHAAKGMVLNFTI
                     E"
     gene            complement(16931..17875)
                     /locus_tag="VFR82_13160"
     CDS             complement(16931..17875)
                     /locus_tag="VFR82_13160"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEU4502595.1"
                     /translation="MTGRPQFPNEQSATGEFTRQDDAFREWVTADGSSRYPAASGRYH
                     LYVSWACPWAHRTIIVRKLKRLERVVGMTVVDPIRDERGWAFREGPGHSPDPINGFRF
                     LSEVYRATDPDYRGRVTVPVLWDTVTKRIVSNSDDDLMRMFNNEFNAFTPSRLDLYPE
                     PLRDHIDEWNALLYEQVNDGVYRAGFATSQAAYEKAVRRLFETLDQLEQRLADRRYLF
                     GSQLVETDWRLFVTLIRFDAVYHGHFKCNIRRIIDYHNLFGFLRDLYQTEGIAETVNF
                     DHIKRHYYVTHDDINPTRIVPLGPEQDLSAPHGRAKFD"
     gene            18165..19424
                     /locus_tag="VFR82_13165"
     CDS             18165..19424
                     /locus_tag="VFR82_13165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4502596.1"
                     /translation="MLRKIMAESNPANFKRLREQADQLSRTTSHQLPKLTPQSVETLV
                     HQLRVHQVELELQCQELLQAHEEAEESRNRYRELYEAIPIGYVTIDGAGRIYDLNPAG
                     ASLLGTRAECRLPNFFSFFADGDAEAFHLVRRVLSNKHAEAHDLKMKSIQETNFLVSV
                     HAAPVHIGEGKGERVRIAFKDITHRKQVEESLREHQVELENNRVELQELMAKLFTAQE
                     EERRRIACDLHDDHCQRITALILEASALEKLVKPLMPSILPRMAIFKQKLGDILDDFR
                     HLTHELHPRHLDTVSLACSMRTHVKEFIEYSELQVEFTEFDMPAHLPMPITICLYRLL
                     QESLSNIRKHAKATHVTIQLSGGKQEVELLVSDDGTGFKCADNKKGLGLTSMQERVRP
                     LRGRVAIDSKPGVGTCVSVRIPLTSES"
BASE COUNT         4303 a         5410 c         5692 g         4103 t
ORIGIN      
        1 atatctcgat acctatcggg gcgtgagaat atcccgaagc ggccggcgct tccttgtcga
       61 aggcgcggtc atttggaacg tcctcgatgc ccaccggcaa cagatcgggc aagccgcaac
      121 gttcgcccac tggacgtggc tgtcataacg actgaggccg acattacccg tcctcctcgg
      181 aaggctggac cgggaggcgg gtgaagcgca gaacggaacg gccgtcgacc gtgaccgtat
      241 cgagaaacat cgctgcaggc cggacccaaa gccctcgttc gccatacaag gcgcgataca
      301 cgacgaagga ttcctccgtt tccgaatgcc gggcaacccc aatgacttcg tagtcatggc
      361 ctttgtagtg ccgatagcgg ccgggcgtga tcatcgtgta ttctttcgct gatcaccata
      421 tataggtgat gacgatggga aagcaaacgg cctcactcac gttcgcttta ttggcggccg
      481 tcggtctcgc cgcgacggcg tcgtcctgcc agcaacagac ctgggaatca gtcatgaccg
      541 ccggccagga ggcggtgcgg caagggaact acgcagaggc cgagcaaatg ttccaggtgg
      601 ccgtgcaaaa ggctgagacc ttcggtcgcg aagatcgtcg cgtggccgtc agcctttcgc
      661 agttggcgca ggtctatgcg ggacagggta aacacgttga ggccgaaccc gcctaccttc
      721 aggcgctgaa gatctatcag gctgtccatg gcgagaatca tgccgatgtc gcggcgacat
      781 tgaacaatct cggcgtgctc catcgtatgt acggtcagta tgcggaagcc gaaccgttac
      841 tcagccgatc gctcgcgatt aaagaacgac tgctgggacc gaaccatccc gatgtcgcgc
      901 tgagcgtcgc caacttgggg cagctgtacg tggcccaggg tcagcccgca aaagccgagc
      961 ccttgtaccg tcgtgcattg tccatcaagc gtgaggctct ggggatgtcg catcccgaag
     1021 tggccaagac cctcgaggat ctggccaacg tcttgcgcaa actcggtcgc agccctgagg
     1081 cagtggctct ggatctgcag gcacgggaaa tccgcgcgaa gcggagttga agatgcgcgg
     1141 tattccgttg tagcgtattc gaatgccgag tgagaatcag ccgctctctc cgaactggcg
     1201 tatgaacggc atggtggcgc gctttgtcgg gatcgtcgcc atcgcgctgg gtttcatgtt
     1261 aggcatgtac ggcctggatg agcagggctc gcgctggctg cacacggcac tcggactgtt
     1321 ggtcaccggt atgctcgctc aagggtatgc gctatactgc tcgatcaaac gtatgcaaca
     1381 acttcacgat cgacccaaga catgaagcgt gccggtagaa cggttccttc cctttcaatc
     1441 tcgctcctgc tcgtgttcgc cgccctcgca gcctgcggcg gtccctggcg cgacggttat
     1501 ttcaagaagg gtgtcgaccg actcacgcag gacgagatct cggaaaaatt aggtcctccc
     1561 cacacggcca agacgcccgc tctgggcggc gatacgatct ggacctaccg tgtcccctac
     1621 agcgacaaag atctggaccc gatggatctg tccgggatca cgaatgcggc aaatgaggcc
     1681 gcatcactga ttggcaaagg ggcgcaagat ggtcccaagc cgaccctcta ttgctatcgc
     1741 tataccctga cattcaacga gcaaaaagtt cttaaacgtt ggaagcgaga agagtgtgta
     1801 cccggtaccc gcgattctct gcatgccggc tagctgaagg tcgcgtcctc tatgatcaca
     1861 tggcttccgc aggtcgatag ctcgatcgtc atggacagcc tgaagtccct gctgttgctg
     1921 ttgtccgtcc tcattgcacg cacgctcatt gtccgctcga tctccaggaa tgccgccctc
     1981 accatggaag ccaagcgccg gtgggtcgtc tccgcgcgaa attcaatggt ctttatcttt
     2041 ctccttggcc tcgtcgtgat ctgggcccac gagctgcagg cattcgccgt gtctctggtt
     2101 gcgctggcgg cggctctggt gttggccacc aaggagctgc tcctctgttg gagcggcgcg
     2161 gcattgcggg tcggcggaaa agtgtacatg gtgggcgatc gaatccagat tgccggccat
     2221 cggggcgtcg tgctggatca tgatattttt gcgacgaagc tgttggaaat cggtccgggg
     2281 caggcctcac atttgtatac cggccgcgtc acggtttttc ccaacagcct cctgtttacg
     2341 aaccccttga tcaaagaaaa cccgaaccag gagtacggcc tctatacgct gaccgtcccg
     2401 ctcgcggctg gggaggattg gaaacaggcc gaggcgcggc tgcttgaggc tggcagggcc
     2461 gaatgcgctt ccttcatgac ggaagccagc cggcagatga aactcctcga gcaacaaaac
     2521 ctcctcgagg ctccgtctcc ggaaccgcga attacgattc aattgtcgga cgtgggccga
     2581 atcgtgctcg tgctgcgttt tcccgcccct gaccgaggcc gatcgcgcat tgagcaggcg
     2641 attctgcggc ggtttttagc ggacgcctga gacgtgttgg tgcgcctgga ggtctccgag
     2701 cctggctctc ttgacagtcc gtggtgctcg tgcgactctg gccgctctca tgaccgatcg
     2761 tgcagttcat cgctggattt ggaggtcccc atgcgaacca tgctgaccgt ggccgttgcc
     2821 gccctatggt tgacgaatgt gactgtgctg gccgttgccg acgacgccat cactggcgag
     2881 atgaaccaac tgaaagccga catcaaggcc gaaaaggaag cgatgaaggc cgacacaaag
     2941 acgaagaaag ccgagatgaa gcggttgaag aaggaacaca agcaaaacat caaggccaag
     3001 aagcgagaga tgagagaaaa gaaaaaggcg aataaggacc ggatgaaggc gcagaaggat
     3061 gccatgaaga cgcagggcca ggatctgaat gctgccggac aggatatgaa acctgccatt
     3121 ccggatcttc ccgcaccaca agctcccggt gcgcagtaac ctcgaccagc cgtctacagc
     3181 agactgacac gatcacgggc cgccaaggcg gcttcggccg actatcccac tgccgttacc
     3241 ggctcgaacg tcatgaaaat cgccacctat aatgtgaatt cgcttcgaag gcggcttccg
     3301 gtcgtcctcg actggctcgc gaaagaaaaa cccgatgtct tgtgcctgca agaaaccaaa
     3361 gtccaggaca gcgagtttcc tctcatggct ctcctgccgt cggggtatga aatcagctac
     3421 cggggcatga aaagctacaa tggggtggcg gtgctgaccc gtacaaagcc ggaatcggtg
     3481 cgggctggtc ttgacgatgg tggagagccc gacgagccgc gacttttgca tgtcgtcgtg
     3541 aacgggatcg ccgtcatcaa tacatacatc ccccagggat tcgagattga ttcacccaaa
     3601 tacgcctaca agcttgagtg gttcaagcgg ctgcgcgcgt acttcgagaa acatctttca
     3661 ccgtcgttgc ctgccatctg gtgcggcgac atgaatgtgg cgccgagagc catggacgtc
     3721 cacagtccag aaaagcatct caagcatgtg tgctatcacg aagcggcgcg ggaggcgtat
     3781 gcgtcgacgg tcgcttgggg attctacgat gtcttcgtga ggctctatcc ggaacggcag
     3841 cagttcacct tctgggatta ccgggcgccg agttcgctgg atgccaacaa aggctggcgc
     3901 atcgatcaca ttttggcgac cgctccactg acggacaaat gcctgcgggc tgaagtcgac
     3961 gtggagccga ggcggtgcag ggatgcttca gaccatacgt ttctttgggc ggaattttca
     4021 ctgccgtgac gttccactcc tcgcgcgttc tcaacgttat cgctccacac cgcttggacg
     4081 cgggcaagtg aggctagcgc tcaattgacc atctgcttgt acccggatcg gaacaaccac
     4141 ggtcctgcgc ggtccatcca tcaaattcac gagccgggca acgcgtgcgc tgcttacgcg
     4201 accggaaatt cgcgatcaac ggcgagtgtt gacgacaatg gctccctgct tcaggtacag
     4261 gtcctatcag tcctcgaaca gcaggcaatt gagccactgg ctgtcggacc aatacgccca
     4321 agcgggtctg gcagttagac caatatcact cgccgagggc gcgtctgaga agaggaagaa
     4381 caactgcgtg tcagaacggc gaggaagatt ggtaggaccg gtgatgccag ttttcgagcg
     4441 taagaaatgg ggaaaatgcc catattggca gccgacccct cccaatcgtg aacgtgtcaa
     4501 agatcgtgcg aagagccggc agccggcggt aaacggtgaa ttttgaatat gagggtgagg
     4561 tcagagacag atgtcgggct gcgttgagcg gaatggtcgc cagtttgtaa cccaagcaaa
     4621 cggagtctcc ggtcaatggc gagcgcgaca tccgtgcctg ccctattcga cggagtgcaa
     4681 gtcgttcact ccgccgaacg agagaatcac atgctgtcat gcggcatgca tcgtcgcgtg
     4741 agtggcgcgg gtgcgggcaa gccggttttg ctcgatcagc ggatcaagaa caagtccgca
     4801 gttaatgcag cgcagcacag tgccgcctgt ggagtagtcc ggtcgcgatt ccgcgatcat
     4861 gagtccttta catttttggc aagtcatctt cacacctcct gcattggatt gcatgattat
     4921 tcgagcgcga atgtaacaac ttaagattaa cggggtgtta atgaaccgtt aaaactctct
     4981 aatgtgaaac accaatgagg tgatcaagaa gacattaatt accaatcact taagagagta
     5041 tgactgagct aggggccgac cgagtactgc tctttcacga aggatggcct catggaagtc
     5101 gatcctacga tctcgtgagg tccgtccctt aatgaccgtt atcgcccttc tgattggctc
     5161 gctttcatag tggcggcggg aggaacggtg gcgatggata gggccgaggt gatcagagaa
     5221 agaagatctt tgccaagtac tgcgctggat gccacggatc acaaggacac ggtgacggct
     5281 atcaactgct cggtcccagt ccggcaattt gatgtcgcag gcgacagaag ggcggtccgg
     5341 ctgggattta atccgcacga ttcatgaagg caagccaaat atgccggcat ggaagcatcg
     5401 gctttcacgt caagatagcc aggatgttct cacctatatc cgttccctct cggccgggaa
     5461 gtaggcccag agctttagtc tttcctgacg gtgacgatga tgtctgaccc cgacgatgcg
     5521 acaagcccga aagcggccaa gcctgaacag ccaggaaccg atcaacgtgt caaggccaac
     5581 cgagatggga ggttcttgtc cgatgagttg gaggggatac cgaccgcggt cccgctagaa
     5641 ggcgacgggt atcggaaggc gagaaaattg gccccggtcg gcgtacgcgg tcagggcctc
     5701 acgatagagg atcttgagcg gatcaatacg cgcagaggac tcattcagct tctccagcag
     5761 aaggacgtcg atcttgaagc agtgcggaag atccttctct atgaagaaga actcaaatgc
     5821 ctgcaagtgg aaatggtccg gctgcaacgg tgggttcaga gcagcggcca gcgaattgcc
     5881 atattggtcg aaggaaggga tgcggcaggg aaaggcggca ccatccgacg gttcaccgaa
     5941 cacctgaacc cccgcgcgat gcgcgtggtt gcactgcccc ggccgactga gcaggaacgc
     6001 ggccaatggt attttcagcg gtacattcag caattgccca acaagggcga aattgtgttc
     6061 ttcgaccgaa gttggtacaa ccgcgcggtc gtggaaccgg tcatggggtt ctgcacgaaa
     6121 aaggaacacc aacggtttct ccagcaggtc actgaattcg agcacatgct ctacgaggac
     6181 ggtgtcatgc tcatcaaatt ctggttttcg atctccaaag aagagcagca gcagcggttt
     6241 gaggcccgac gaaacaaccc gctcaagcaa tggaagctca gccccgtaga cgaaaaggcg
     6301 caggaattgt gggacagcta cacacgatac aaagaagaga tgttcagcaa gacgcacacg
     6361 accttcagtc cgtggatcat cgtcaaagcc aatgacaaac aggctgccag gctcgaaagc
     6421 ttgagatacg tgctgaatct tcttccgtat aaggggaagg aggaaagcaa gacgcgcctg
     6481 gcccctgacc cgaatgtcgt gacgcggtac catcgcaaaa tgatcgaact ggacttttag
     6541 gccatcatgt cagtggaggc aagacaatcg gcgagcattt cccagaggag cacgaccgac
     6601 tcgtgacatg aaatcgaccg gctggccgaa cgacggtgcg ggtttctttc gagagttgga
     6661 attgctcgcg cccgaccgct atgcggcgcc gttgcggcgc gcgccataag aactagctct
     6721 acgctatgaa gggtttggcc ctcctgaacg taccaccagc accggacatg agacgtggtg
     6781 cacgatcgca tgagagacgc tccccaggag aaaccgatcc agcccttccc gtccatgcga
     6841 accaaccaca acgagatcga actccttcgc agtctccgag atcacaggtg ccggctcacc
     6901 cttcatcacg cgactcgacg cactgtagtg tgacgggagc agcgcctcgg cgcagactct
     6961 aacgctcgcc tgcgcttggg tcatcaattc ctctgaccag gattcgaatg gcgggatggg
     7021 cgcgagttcc ggcatcggcg tcgggagcat aggcaccgcc gtgagcacgg tcgcttctac
     7081 cggagtgcga aaaggatggc ggctcagcca ctcgcagatt tgacggccat cgtcacgtcc
     7141 ctcgatagca actaacacac ggcggatggg tttcgcatgg cctttgacga gcaaggttgg
     7201 ctgcatcgca tgcaacagga cacggtggga cacgctggtg agcacgagtt cggcgactct
     7261 cgaatgatca tgcgtgccga cgacgattag gtccgcgttc atggccgtgg cggtatcgag
     7321 gaggaaccgt gccggtttac gaaactcgca gagctggcgt ggcttaggaa tatcgttcgg
     7381 cagcatcgta gcggcccggt ccagcgactc gcagccggct tcgaacatcg attttcggaa
     7441 gatatcgtac ccctgaagat tggcggcttc ggcgacgatg gggtattcaa agagtcccaa
     7501 atcgacgccg tggaccagcg tgacatctgt cgggtggtag agcaatccga gctgttcgac
     7561 cgccgcgaag gccgcatcgg accagtctac gcctgcaacg atcttcatgt atcaccttcc
     7621 ctctgcaacc aggtgttgtg tcaaccactg gccgcctgcg cgccttgcgc ccgtcttgcc
     7681 gccttcagat aggccaggac ctgcgcatca tcaacctgcg taaaattgtg gtagtgatgg
     7741 ccgaccgcca cgaacggttc cggcgttgca atcacgatca attcgtctac gcgattccgc
     7801 accaaagcga ggctatccag gggcgcgacg gggatggcag cgatcagtcg acgaggagat
     7861 aattcttgga cagcttcgat ggttgcaaag aaggtggcgc cggtcgcgat gccgtcgtcc
     7921 accaggataa cggtcttgtc tcgaatcggg gcaagcggca atccgtgacg gtacagctgt
     7981 tgccttcgcg cgagctcctg ccgttcattg ccgatgatcg cttccagatc ggagtgtgag
     8041 agatgaaacc cttcgaccgc ttcaggatta acatagatag cgccggtttc gctgagcgca
     8101 ccgattgcat actcgggatt gtccggcgta ctcagcttcc gagtcacgaa cacgtcgagc
     8161 gccagatgca gagaaacgct cagttcgtat ccgactgcca ctccgccgcg tggtagcgcc
     8221 agaataagtc cgctggggtc gccttggtag gattcgagct tctccgccag tagtcggccg
     8281 gcttctgctc gatcgcggaa caccacaacc acctcgttca atggtgttct ctttacatga
     8341 ggaacttcta tgtcactgtt ttactcagct gtcgcgggca atggcgacag gtgatatccc
     8401 tggcacggtc aacggacggg aaaactgagc cggttctcga aagaaccttg aagagctgag
     8461 cactgtatag cggacggcat acctcaacga gaggcgttga gaacgttggc tatgcccaag
     8521 caggaaccgc cggctctgct acgcacatga cgctgcgttg tgccggactg atggtgcagc
     8581 cggatgcaca tgcctatgca tggggccata ggtgcacatg gcaaacacga tgcttctaaa
     8641 gagatgatca gcggcatctt ccattcgcac gaaggccact gctcctgccg caatcatggc
     8701 gttatagagg tacggcgtat actctgcgga cattcccaag atcaccactt gaggaagaat
     8761 ctgcctgatc tgccgggttg catccactcc gttcgcaaga tgagcatcca tcagcaccag
     8821 atcgaccgcc atcgctttgg cttgctcaac cgccgcctcg gcatcagaag cttcaccgac
     8881 gacctcaatg agatcggcat agccgtccaa gatcatgcga atcatttctc ttatcgatgt
     8941 atgggggacc acaattaata ctcgccatcg gagggcagct tcagttttgt tcggaacaga
     9001 cgatggatga cgctcaaacg gcttggttag ggtgatggtc ggcatgtgac ctcctcggct
     9061 ggcagtggct ggattgggtg tacgtacccc ttccacattt tttcgataac tagggaggtc
     9121 cctagtcggt agatgttgat acttaccaac cccttagcgc tcctttgacc gccactcggt
     9181 cagggtggta cactgtgtcg ccgtacgagc tatatgttgt aacctcgcgc tcttcccctc
     9241 ctattacctg tattgcgcga tacgtcttca cgttgctcct acgtgtcgcg gtaccatcgg
     9301 aaggaaagaa agcggttgga cgacgctttc tcattcggat gccgaaatgt gatagcgact
     9361 gatgaaagga atgccgatga aaatttcgca tgtgtggaag tcgggcagcg ccaagaaaaa
     9421 gaccaagccg acctacaaga tccgttggga actcttagga ttgtcccgtc cggatcaagc
     9481 acaatcgagt ctctctatgg aggcaatacg aagcgaggtc tcgcttctgg caacgcaagg
     9541 tttgagcgaa aggactcagt cgagatccac tacaccagcc gtcggcggac gaggactcaa
     9601 gcgaagcatc actggaagcc ggcgtcgggg tgctggaaag taacaaccgg ttccttgtaa
     9661 ccaaggaggg ttaccatcgc acgagcagga aagacaacgc tagccaagcg cgaccgagaa
     9721 aaagctctcc aacagcagcg tcgggacaaa gaagccaaac gagtgctccg caaggccgag
     9781 aaagccgctc ggggcaccgc tcctgaaggg gaagatccgg atttggctgg tcttcagtgg
     9841 gggccgcagc cgccggcgtt ttagacttag attcgctcat ggcgactgcc tcggggcagc
     9901 tcgcctgacg cgagtcaaaa aattccgcgt agccattttc catttatcag aacgactctt
     9961 gcgcggctcc ttcatgcctc ttacaatcgc ttccgctgag aggcgttcat gccgtcaggc
    10021 tcgccgcgtc gattcatccg cccatcgttc tccaaatcgt tatccccaac catagtgatc
    10081 gacaagagat cgcggacaaa atttcttatt gtcggtcttc tgttgctgat cgccggatgc
    10141 tccgtgctgc cgggccattg gcggcccgga tggaacgagc actggcgaga cagcaagcca
    10201 gtgcccgata ccgccggctc atggaaattg accgatcttg aagtggcaga cggtcgtttt
    10261 ttggcgcttg ccatctcggg gggcggaagc cgagctgcta actttggcgc tgccgtcatg
    10321 ctcgagctcg agcaacgcaa gttgttggag caagtcgacg tgatttcggg agtatccggc
    10381 ggaacactcc ctgccgcgta ttacggactg ggcgacaaag cgggcgcctt cacggagtct
    10441 acccttcgaa aaacgctagg attcgacttt cagggcagtt ggatccggcg gtggtttctg
    10501 ccgcaaaaca tttttcgcta ttggctctcc gacttcaccc gttcggacat tatggttcag
    10561 gtttttaaca accagctcta tcacgacgcc accttccagg atctcctgcc gcatccaaag
    10621 atcttgctca attcgacggt ccacaacgat cacacccggt ttacatttac ggacgagcgc
    10681 ttcgccaaac tccattcggt gctggccact tatcccgtcg ccaatgccgt gaacgcatct
    10741 tcagcatttc cgggtgcgtt ccaggatgtg accctgcagt ggtacatgga accgccgcaa
    10801 tacatgcatc tgtatgacgg cggccccatc gacaatcttg gcgtgcaggc cattacagaa
    10861 tacgtcaatc gaaacatcct cggcacatcg ctggatcgac tgtttcccaa cggttgtctg
    10921 atcgttgtca ttgatgccac gccggcgagc gagcaccctg agctcaacac ggggcagtcc
    10981 agccgctcgt tgattgacta cttcgtcgat accaatgcgt tggatgcgat ggatgccatg
    11041 ctcctggagt ccaggcggtc cttgctggca ggcatgggca ttcccatcgc gaggcaggat
    11101 caggagatgc gtggacggct gccggtgaat gacccacggc agtgcggctg cgaagtccgc
    11161 cacattgctt tgcgacagct catgtatgcc ggggaagagt cggatggact ggccgaacga
    11221 gtgacgcaga tcaagacgaa attctggata ggcgaagacg agcaaaatga tctcttcacc
    11281 gccgccaagc tgctcatgaa gatggttgac gacaagaagc tgctggctga ggaatcattg
    11341 aggacgtcgt gcgcagttga acgcaagtga gcggcttagg aacggtcccc gcggatcgtc
    11401 agcatatgcc tgctactgtt ctaccccgcg taagaccgtc gatgaggatg gtgatgccgc
    11461 ctggcaacca tgaataaccg cctcgctctg gggttcagac ccacttgctt ccctgatcat
    11521 cacgcggctt ttgcggttcg actaccctga tagtctgcaa agagagcgat caattcccgg
    11581 ttaaaggcgg gaaggtcagc gggcctgcga ctactgaccc aattgcggtc gcgaagcacc
    11641 tcgatgtctc gccagtcggc accggcgttt ctgagatcat cttgtatcgt atggtaactg
    11701 gtcatctttc gtccggagac gaggccagcc gatacgagca accatggccc atgacagatg
    11761 acggcgattg gctttcctgc ctcgtcgatg gccgtaatga acgacttcgc ttccggaacc
    11821 acgcgaagcg cgtccgcatt cagcgctccg cccggcaaga gtgcggcatc gaactgcccc
    11881 ggatcggctt cggccaacaa ttgatcaatc gtgaaacgat ccgctttctc gtcatgatgc
    11941 atcccttgta cttctcctga tgcgggcgag acgataagcg ccttcgcgcc ggctgcttcg
    12001 agagcccgtt tcggttcggt taattcagat tgttcgaaat catcggttac caatatcgcg
    12061 acccgcattc cctccaactg ccgcggtgcc atactgacct cctttcagag aaaatctatt
    12121 tggtaacaaa ctcggatcga aaggagcact agggaatgac ctggcgctgg tcatccgcac
    12181 aaacgggaat gtgtatttca gccgttgcta gccgacactg cggactggct cgcgtccata
    12241 cgttccacgc cttgtcagct gatcaagcac aggatgcgtc tgaaagaaac accaagcgac
    12301 gttgaatctc acaacagtcg gctaacggag tggcgcggct agcggcgagg gcgcaagcgg
    12361 cgcggcaaga ggcgaggggc tgagcggctt cgccagcggt gatggtggga ggggtgcggc
    12421 cagtggtgat ggcggcaatg ggacggcaag aggagatggc ggcaacggtg tggccaatgg
    12481 tgacggagga agcgtcgctg caagtggtga ttcgagcatc ggcgctgtca ggggagaccg
    12541 ctgagagact gtcgagtccg aggatgcgct cggctggttt ctatgcgatg gaaccacaag
    12601 cggaagagcc gaggggtctg tcttcgcggc aagggcttgt tcgacggcct gcagatgctg
    12661 gtttgcgggg tggagttgcc tggcctgtag caacaggtcg ttgacttgcc gcacggtcat
    12721 cggcgtcaag taagcataat cggtcagcgc tcgggcgacc agtgcgtcgg cttgtatctg
    12781 attgagcaat tgctgtcgct cccgcgtaag ggcttgcacc tgttccgctg tcatggccgt
    12841 gcccaaggct tgattagctg cgtcgagttg caggcgaagc tgttccgttt ccgtccggag
    12901 ggaggcgagt tggaccttgg cgttctcatt ttcccgtagg gcggtgatgg cttgaatgac
    12961 ttcttgttga tcggcttgca cggtgagatc tacatggatg acggccacgc cgttttcgag
    13021 acgtgtgctc gtctgctgat tgaggaccat cacgaggccg gctgtatacg tgcgaatttc
    13081 atcgcgcgtg acatcaaggt ttttgacttc agtgacactc tccagatagg tcgcgacttg
    13141 ctcgagcgca tcgcgtttag cagcctcgat ggccatgcgg gtggcatccg tccttgtgtc
    13201 ataatttccc atgcgaaact caccgcttga cgtgacgatg cgaagcgtgg gctcatgcct
    13261 tcgctgagga ggcggcgggg ggacggtcgc cagaggaacg gcctcctggc ggtatttctc
    13321 aggaatcgac tgcagctctg tggtaaaggt caccgtgcct gagtcgtctc gatagcggta
    13381 gatttgattg cctgcccaga tctgggctgt cgaggtgagg aggtagaata cgacgatcag
    13441 agaaaatatg ctcatgaatc gtccccctga tcaccatacc atactccggt gactgtcggc
    13501 ttgggagtgc cggccgtcgg aattgggtcc agcgatagcg ggattggtga ggaagaaaca
    13561 gctcgcgacg ccaatgactg gctatcgcga gccggatccg gtgcgacaga ttactcgact
    13621 tccaaaatgg tcaaggtgac gaggagcggt ttgcccgcca aaggatggtt atagtccatg
    13681 accgcgtcag tctcagacat ctgggtgatg gtggcctgct ttccggtctt acggtcttct
    13741 ataatgtcgc cgggcttggc tcctgcgggt aattccttct taggcaccat ggttttcttg
    13801 ctcgagtcat aggcgccaaa cgcttggtcc tcgctgagtt tgacttccgt cttctcgccc
    13861 cggtgcatcc ccgtgaccgc ctcctccacc gcaggaagca tctgatgttg tccctggacg
    13921 aactggctaa gatcccggac ctcgaaccgt tcatcacccg gcacagtgat ctggtagagg
    13981 agggtgacct ttgagccgtc gctgatcgcg cgcgtgtcgg cggcgtgacc ggataacgca
    14041 aaaagtccca gagatccgat cagcgccgat agcgctacca ttgtcactgt atatcggctc
    14101 gctttcgcca tagtgcctcc ttaaagaaaa tgaaatcaga cgccaccatg cgtccgatcc
    14161 actgttgcgc cgcaatcttt ttgccaaaaa tctaaacgta atgattgtga ggagttatga
    14221 gcatcggttt tctacggaat cgaaagtaca aagaacagtt cgtccacctt cctgcgaaag
    14281 aactggattg cggccctctt agagtcatag acagtctgtc cctatacgaa agacagactg
    14341 tcttgaacac taggggctcc ccttgtcaat ctgcaattgc ccgtgcttga ctcacgagtg
    14401 agtggcagcc tagcctcatc ggcaacgttt ccatccgtat gagaatcact cgtatgagaa
    14461 tcactgcatc attacagagc cgcatcagtc tattcttcat ctcgtgtctg tggctgcccg
    14521 gctgtgccca cctcgcctat caacacatga acgatccctc tcgggatgcc tggcagaaac
    14581 cgaaagacgt ggtcgagaga ctcgacatta ccccgggatc ccgagtcgca gatttaggtg
    14641 cgggcggggg ttatttcaca tggcatctgg cgagcgctgc gggccctcga ggacgagtgt
    14701 acgcagtgga tatcgatcag acaggcttga gtatcatcac caaggaaatg cagactaggc
    14761 acatcacgaa tgttgtgccg gttcaagccg aacccacgga cgcaaaatta cctgaaccgg
    14821 tggacctggt gttcagttgc gacacctacc atcacctgga cgaccgtgtc gcatacttca
    14881 aatcattggc acgatatctc aagccagatg ggcgagtggc gattcttgat ttccatccgc
    14941 atggcttctt ctcgggcctt ctgggtcatg gttctgcagc tgaggaggtg cggcgcgaga
    15001 tggagttggc cggttaccgg cggacggccg acttcgacct gatcgaccgt cagcattttc
    15061 aaattttctc gagaacaggg ttttgacccc ctcttctcat cacgtatagt tgcgggcatg
    15121 ggctgagacc cgtaagatcg ctgtatggat tcgcaaacca tcacacgcat caaaactaaa
    15181 ttgcccgcac cctatcagtt gacggcgatc aatcaggaca cgcacgacac gaagaccttt
    15241 tgtttcgcgc tgcctgaaga tgcgacgctg gacatgctgg ccggcgatca tctctacgtg
    15301 catgcgacca tcaatggtaa agcggtcaag cggccttaca caccatcctc tgtcccaggc
    15361 accaccggct attttgatct cacggtcaaa cgctacgaga atggttcggt ttcacggtac
    15421 ctgcacgacc gggagatcgg cgacagcgtg ctcatgagcg gaccgaacag cggcggtcat
    15481 tgggtggacg gcatggccaa gcgtgcggga tttgtggcgg gcggcaccgg gatcacaccg
    15541 atgatttcca ttattcgctg gattctcgca aaaggccttc cagtggagtt gtctctcgtc
    15601 ttcgcgaata agagcgaagc cgacattatt ttccgtgacg aatgggaagc caacggccgt
    15661 gagcatgcga acttccacgc ctatcacatc ttggagcaac cgcctgccgg ctgggcacaa
    15721 ggcagaggac gaatcacgga agagacattg cgcatgcacc ttcctgagcc tggtcccgag
    15781 acagttgtct ttctctgcgg cccaccgatg atggtcgatg ccttagaagg gacactcaag
    15841 ggcatgggct atccggaaca gtccatcatc cttccttagc gattgatctg ccggtccacg
    15901 cttgatgctg cggacctcgg cgcctttgca ccacagacac gccggacatc cgctgaggga
    15961 tctccggctt attcgatggt gaagttcagg accatgcctt tcgcggcatg cgattcgctc
    16021 atgacggtgt tggagaacaa gcacatgatg gcgtagcggc ctggtgtcag gtctgcggta
    16081 aaggtaccgt ctcgtcccgg ttcgagtccg ctcattccgc cgatcagtgt cccgggtaag
    16141 cgggtagggc tgtcctgatg gatcgcggtc agcacgtctt cggttgaagc gcccggatcg
    16201 agacggatga aactggcttg atgggtctgg ttccctcgat tcaccacgta gaaggtatgc
    16261 cgaccctttc tgagcggttg cacgaccaca aattcatatt caaacatcgc catatggaag
    16321 ttgccccgga agtcgggcgg cggcgatttc gtatcgatca cccgcaacgc cttctgcatg
    16381 cccagcgcgg cgtgagtctg gtgggttttc cccggaatgc cgcaaatgat gacgtaagag
    16441 cctggttcca aatacaacgt ggcttcagcc gcctggcctg ctccgaccgc attggggccg
    16501 cccacctgtt tggcccacgg agggatgctt tgcgagcctg attggagagc cacagccaac
    16561 tccgccggtg ttttaccggc atccaatttc agaaattgaa gttgatgcgg ttccttgccg
    16621 cggttgcgca ggcggatggt ggtttggccg gcagaaatcg tttcagggcc gtcaaaacga
    16681 tggtccctgg cgtaaaaggt tgcttccttc aatgaagcgg cgctgactga gtgggagtag
    16741 agcgtggcaa cggcgatcag tatgcagaca caacactcgc cgaaccggaa agcattccgc
    16801 atggttcctc cctgagaacg acgctgtttc tcgcgacctc agcgatgggg tcgaccaacg
    16861 gaccggcaag agtagtaata actttaccta caagatttag caatagagcc attcggatcg
    16921 gtgggctcct ctagtcaaat ttcgcccgtc catgaggggc cgacaagtcc tgctcgggac
    16981 cgagagggac gattcgggtg ggattgatgt cgtcatgcgt aacgtagtag tggcgcttga
    17041 tatggtcgaa gttgaccgtc tcggcaatgc cttcagtctg gtagaggtcg cggagaaagc
    17101 cgaagagatt gtggtagtcg ataattctcc ggatgttgca cttgaagtgg ccatgataaa
    17161 ccgcgtcgaa ccggatgagt gtgacaaaga ggcgccaatc cgtctcgacg agttgcgagc
    17221 caaacaagta ccggcgatcg gcgagacgtt gttcaagctg gtcgagcgtc tcgaagagcc
    17281 gtcggacggc cttttcatac gctgcctgcg aggtcgcgaa ccccgcgcga tatacgccat
    17341 cgttcacttg ctcatacagc aatgcgttcc actcgtcgat gtgatcccgc agcggttccg
    17401 gatacagatc gagacggctg ggggtaaagg cgttaaactc attattgaac atccgcatga
    17461 ggtcatcgtc ggaattcgag acaatgcgct tcgtgaccgt atcccagagc actgggacgg
    17521 tgacgcgtcc gcgatagtcc ggatcggtcg cgcgatagac ttcgctgaga aagcggaagc
    17581 cgttgatggg atccggcgag tggcccggcc cttcgcgaaa ggcccatccg cgctcatccc
    17641 gaattggatc gaccacggtc atgccgacga cccgttccag ccttttgagc ttgcgcacga
    17701 tgatcgtgcg gtgtgcccac gggcaggccc atgatacgta cagatgataa cgaccggagg
    17761 cggctggata gcgggaactg ccgtcggcgg tgacccactc gcgaaaggcg tcgtcttgcc
    17821 tcgtgaattc acccgtcgct gattgttcgt ttgggaattg cggtcgacca gtcatgagag
    17881 gctctgccgg caagtatggc acagagcaca ggagagacaa caggccaaca tcaggacact
    17941 attcaacagt cacgccacct ccttccgcaa gaaatgcggg ccacagctgt gctagggact
    18001 caccgcgttc aagagcggtc cgtaatttgc aactataacc tgttgcatga ctcttctact
    18061 ccagagcctg gaagttgggt tgtaaaaaag aaggagcagg caaaagggac ggctttcggt
    18121 gaccctcatg gctaccggtc gtgcgcggag cggcgaagcc gtcaatgttg cgcaagatca
    18181 tggcagaatc caatcccgcg aatttcaaac ggttgcggga acaagcggat caattgagcc
    18241 gcacaacttc ccatcagctg cctaagctca ccccgcagag tgtggaaaca ctcgtacatc
    18301 agttacgtgt ccatcaggtt gagctcgaat tgcaatgcca ggagctgctg caagcgcatg
    18361 aggaggcaga agaaagccgg aaccgctatc gagagttgta cgaagccatt ccaattggct
    18421 atgtcacaat cgacggagca ggcaggatct atgacctgaa ccccgccggt gcttcactgt
    18481 tgggtactcg ggccgaatgt cggctaccaa acttcttctc cttcttcgcg gatggcgacg
    18541 ctgaagcgtt tcatctggtc cgtcgggtgc tgtcgaataa acatgcggaa gcgcatgatc
    18601 tgaagatgaa aagcatccag gagacaaact tcctcgtgag cgtccacgct gcgcctgttc
    18661 acatcggtga gggcaaaggt gaacgcgtga ggatcgcttt caaggacatt acccaccgca
    18721 agcaggtcga ggagtcttta cgcgaacatc aggtggagct cgaaaacaat cgggtcgaac
    18781 tgcaggagtt gatggccaag ctattcacgg cgcaggagga agaacgccgt cgcattgcct
    18841 gtgatttgca cgatgaccat tgccagcgta ttacggcctt gatcctcgag gccagtgccc
    18901 tcgagaaact ggtgaagccg ctcatgcctt cgatcctgcc gcggatggca atctttaaac
    18961 agaagctcgg cgatattttg gacgatttta ggcatctcac ccatgagttg cacccgcggc
    19021 acctcgatac ggtgtcactc gcctgttcga tgcggacgca cgtcaaagaa ttcatcgagt
    19081 acagcgaact gcaggtcgag ttcactgaat tcgacatgcc ggcgcatctg cccatgccca
    19141 tcaccatttg tctctaccgc ctgctgcagg aaagcctcag taatatccgg aaacatgcga
    19201 aggcaacgca tgtcaccatc caactgtcag gcggcaagca ggaagtcgag ctgctcgtgt
    19261 cggacgacgg gacggggttc aaatgcgccg acaacaaaaa gggcttgggt ctcaccagca
    19321 tgcaggagcg ggtacgaccg cttcgtggcc gcgtggcaat cgacagtaag cctggcgtcg
    19381 ggacctgtgt ctccgtcagg attccgttga cttccgaaag ctgaccgaag agtggatcga
    19441 tgagatctct gctttctcgt tcaccgaatc acagcaccct tccatgccgc tgagtcgtcg
    19501 tatgaacg
//
LOCUS       DASTQY010000405         2677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_508946, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000405 DASTQY010000000
VERSION     DASTQY010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508946"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VFR82_13170"
     CDS             complement(<1..733)
                     /locus_tag="VFR82_13170"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU4502597.1"
                     /translation="MQVLVTTDGSKYGKWALEWVGQLPLAEEPTVKVLHVVDIGRLRA
                     PFMLQPAVIGTERYLKAEITKLQTQAERTKHEAARWLKSAGLKGRVVIERGPVAATIV
                     KAAKRGVTLMAVGSRGLDALDRFMLGSVSTHVIHHVSCSVLVVREAPRPIRRVVLAVD
                     GSTASKRALQFLLRAMRPSTGRPSQGPIRVTVTHAMPFLKYPEVQQVGKAVVEACSTK
                     LVRAGYVVEQMPIIGNPADKILKTAE"
     gene            complement(752..2011)
                     /gene="pfp"
                     /locus_tag="VFR82_13175"
     CDS             complement(752..2011)
                     /gene="pfp"
                     /locus_tag="VFR82_13175"
                     /inference="COORDINATES: protein motif:HMM:NF041103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HEU4502598.1"
                     /translation="MSASRPIVGMLVGGGPAPGINSVIGAVTIRSILGGSEVLGFVDG
                     FKWLMEGSTGHVQPLSIEEVSRIHFRGGSHLGTSRANPTKRPEHLDRVLSTLMSLGIT
                     RLVTIGGDDTAFSALKLEARSEGRMQVVHVPKTIDNDLDLPYGIPTFGFQTARHYGVE
                     ILKNLMVDARTTSRWYLTVTMGRKAGHLALGIGKAAGATLTIIPEEFRERPVRLQRLV
                     DVLIGTMVKRFASGRTDGVIVLAEGLIEIIDQDDLGGLDHVERDEHGHLRLSEVDIGD
                     LLRREVTKGLKALGLSVTVVSKNVGYELRCADPIPYDMEYTRDLGYCAAQYLLDGGTA
                     AMVTIQDGRFAPMPFKEMLDPVTGRARVRMVDTESQSYQIARQYMIRLDDEDFTDQER
                     LNRHAAVVRLSPDAFTQRFQLNRGKRG"
     gene            2207..2572
                     /locus_tag="VFR82_13180"
     CDS             2207..2572
                     /locus_tag="VFR82_13180"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU4502599.1"
                     /translation="MISKRTAVCGLFLLLGLTLPFRLAAQEYPPDASRGKAVYERHCL
                     SCHGVQGRGDGPNAVLLTIAPANFHRPGSFLKSDETLLRMIEHGGVYSPMHAWRGRLT
                     DGEMQDVLAYIRLLSQDAS"
BASE COUNT          535 a          842 c          752 g          548 t
ORIGIN      
        1 tttcggctgt tttcaaaatt ttgtcggcgg gatttcctat tatcggcatc tgctcgacca
       61 catagccggc tcgcaccaat ttggtcgaac acgcttccac gacggccttg cctacctgct
      121 gcacctccgg gtacttcaag aatggcatgg cgtgcgtgac ggtgactctg atcggtcctt
      181 gcgaaggccg gccagtcgag ggtcgcatag cccgcagcaa aaactgcaag gcccgtttgg
      241 aggccgttga gccatcgacg gcaaggacga cacgccggat cggtcgcggg gcttccctca
      301 cgactaatac agagcaggac acatggtgga tgacgtgcgt ggacacgctc ccgagcatga
      361 atcgatcaag ggcatccaag ccgcgagagc cgacggccat gagagtgacg ccgcgcttgg
      421 ccgctttgac gatggtggcc gccacgggcc ctcgttcgat caccacgcgc cccttcaagc
      481 ccgcagactt caaccagcga gcggcctcgt gttttgtgcg ctcggcctgc gtctgcagct
      541 tcgtgatctc ggctttcagg taccgctcgg taccgatcac cgccggctgc aacataaagg
      601 gcgcgcgaag tctcccgata tccacgacgt gcagcacctt gacggtgggc tcctcggcga
      661 gcggcaactg tcccacccat tcgagcgccc acttgccgta tttggatccg tcggtcgtga
      721 ctaacacctg catgcggtct cgcctctctt tttatccgcg cttgcctctg ttcaactgaa
      781 accgctgcgt gaaggcatcg ggcgacaacc gcaccacagc cgcgtgacgg ttcaaccgtt
      841 cctgatccgt gaaatcctcg tcgtcgagcc ggatcatgta ctgacgggcg atctgatacg
      901 attgcgactc ggtatccacc atcctgactc ttgcgcggcc ggtcacgggg tccagcattt
      961 ctttgaaagg catgggcgca aaacggccat cctggatcgt gaccatcgcc gccgtcccgc
     1021 catccagcag atattgcgcc gcacaatagc cgagatcgcg agtgtactcc atatcatagg
     1081 gaatcgggtc cgcgcatcga agctcgtagc ccacgttctt cgacacgacc gtcacggaca
     1141 gccctaaggc cttcaatcct ttcgtcactt cgcgcctgag caggtcgcca atgtccactt
     1201 ccgacaggcg caagtggccg tgctcgtcgc gttccacgtg gtccaaaccg cccaggtcgt
     1261 cctgatcaat gatttcgatc aatccctcgg ccaagacgat cacgccatcc gtccgcccgc
     1321 tggcgaagcg cttgaccatt gtgccgatga gcacatccac cagccgctgc aatctgaccg
     1381 ggcgctccct gaattcctca ggaatgatgg tcagcgtggc gccagcggct ttgcctattc
     1441 ccagcgctaa atgcccggcc ttccgcccca tggtgaccgt aagataccaa cgcgacgtcg
     1501 tacgggcgtc caccatcaga ttctttagta tctcgacccc ataatggcgg gctgtctgaa
     1561 agccgaaggt gggaatgccg tagggcaggt ccagatcatt gtcgatggtt tttggcacgt
     1621 gtacgacttg catgcggccc tctgagcgcg cctccaattt gagcgcagag aacgccgtat
     1681 cgtctccacc gatcgttacc agccgcgtga tacccaggct catgagcgtg gacagaaccc
     1741 ggtccaggtg ctccggcctt ttcgtcggat tcgctcgtga ggtgcccaaa tgtgagccgc
     1801 cgcgaaaatg aatgcggctg acctcttcaa tagagagcgg ctgcacatgc cctgtgctgc
     1861 cttccatcag ccatttgaac ccgtcgacaa agcccagtac ctccgagccg ccgagaatgc
     1921 tgcggatggt cacggcaccg attacactgt tgatccctgg tgcgggacct cctcccacaa
     1981 gcatgccgac gatgggacgt gacgcactca tgatctcaag actcctgtat taccaacggt
     2041 aggggtatgc cgcatggcaa tcgcggctgc ggtgagaatt atcaggattc agccgttgcg
     2101 gcaagaggca cctggaggat ggcgggtcgc cgaggcggct tgaaaagcgc tcgacaattg
     2161 atcacaattc ccgaaaaaca agaacgggct acaggtcttt tcattgatga tcagtaagcg
     2221 tacagctgtc tgcggtctct ttctgttgct cggcctgacc ctgcccttcc gtctcgcggc
     2281 acaggaatat ccgcccgatg cgtcccgtgg caaggcggtg tacgaacggc attgcctaag
     2341 ctgccacggc gtacaagggc gaggcgacgg gccaaatgcg gtcttgctca ccatcgcgcc
     2401 ggccaacttc catcggcccg gttcgttttt gaaatcggac gaaacgctct tgcgaatgat
     2461 tgagcacggc ggcgtctaca gccctatgca cgcctggcgt ggcaggctta ccgacgggga
     2521 gatgcaggac gtgcttgcct acatccggct cctatcccaa gacgcaagct aggcccctga
     2581 atcgtcctgc caacacgtcg ttcatacgac gactcagcgg catggaaggg ttctgtgatt
     2641 cggtgaacga gaaagcagag cacgagagag agatctc
//
LOCUS       DASTQY010000406         6268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1350195, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000406 DASTQY010000000
VERSION     DASTQY010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350195"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..850)
                     /locus_tag="VFR82_13185"
     CDS             complement(155..850)
                     /locus_tag="VFR82_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4502600.1"
                     /translation="MLTNQPASLPQSQSIELPDQQGVKLKILLVDDHAIVRVGLRQML
                     LDAFHQLDVTEAGTGQEALSKVQQAAWDLVVLDINLPDTNGLDVLKKIKTLRPALPIV
                     VLSFHPEEQYAVRALKSGASAYVTKDTAPQELDTAIKTVLAGHQYVTPSLAQRLEAMP
                     IQSGGKPLHTLLSDRELQVLSMIGGGKSVTEIAVALDLSVKTVSTYRMRLLSKLQLKT
                     TSALIRYALTHHL"
     gene            complement(987..5081)
                     /locus_tag="VFR82_13190"
     CDS             complement(987..5081)
                     /locus_tag="VFR82_13190"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4502601.1"
                     /translation="MTALRSRFVHPLPPLAGLLSLEVGALVLFGWAVNSTDLKSLAPG
                     LIPMMPNAAIAFVFSGISLLAASMRTPRQHVRFVAGGAAAVTAIIGLATGLEYLLMVD
                     LSVDQWVLYEPLEAVGPSIPGRMGINTAVCFLLLGLALALKILSQGRLVGVSDGFSLG
                     ATFLGFLAFLGYLYQERFLYGVGPSTPMALHTALTLMLVGAGALALEPAKGLMAVINS
                     NQAGGYMLRRVLPGVFVILPALGWIRLYGERQGWYGTDMGVAIFATTATVTLAALLWG
                     TARRMNEADQARRQSDRLFAAFMTHLPALAWIKDIQGRYLYFNDAFTHAFGVRLEDWR
                     EKTDCEILPDHTAAQFQANDAQVRATAKAVVAVETAPYKDGLHQSLVSKFPIVDEEGN
                     LALIGGVAVDITERNKIETALRETEEHFRQVTEHIQEVFWLSDTAKNAVLYVSPGYES
                     IWGRSCESLYRSPRSWLEAIHPDDRQRVLEAALAKQMTATYDEEYRIIRPDGSMRWIR
                     DRAFPIHDSSGSVCRIAGIADDITERKRAEEALQRAHHELERRVAERTAALRASQERL
                     EMAFHGAGLASWDWQIATGAVSFNERWAQLRGFAVHEMAPHVSSLLNGIHADDRSMVE
                     MNLNACLRGETPEFEAEMRVQTRANEWTWILSRGRVIERSALGAAVRMAGIEIDMTAR
                     KRTEEALGESEARYSSLVSQATDIIYTAGVDGRFTFVNAAACALMGYEEQELLGKHYL
                     ELIREDARDRAQQFYKDQLLAQVPSTYYEFPAMTKTAEEVWFGQHVRLRLVEGQLVGV
                     EAIARDITARKMAEQALQERAKCAAFAAEVSLLLNHDEPLDCLLQRCTDAAVEHLGAA
                     FTRVWLLEPGDLCADCYKASSCLDRTTCLHLHASSGLSTNLNGEYRRVPLGALKIGRI
                     AQGDGALFTNDVLHDDRLPNKEWMREHGLHAFAGFALMVEGRVFGVLGLFSCEIISEP
                     IRQTLESVCNGLAASIARKQAVAALQASEARTRAIVESALDAVVTMDERGIITGWNSQ
                     AAAIFGYQEREISGKLLSDTILPTRYREAHSNGLQHFLSSGHGRILNRRVELSALHKD
                     GSEFPIELSVTALTIEGRKVFSAFLRDISERKQAERALKEAYEQLRDLTGRLTEAEEI
                     ERRRLARELHDEFGQALTGLKFDVAWLTKEMSRVKKVPDVAGIKAKTMGMAHAIDGLI
                     KSVRATAASLRPGVLDDLGLVAALEWLAASFHERTGWPCDLMIDSSARDVSPDLALAT
                     TVFRGAQELLTNVMRHAQASKASMRLTAGEGQMVLTIGDDGRGFTPDQWKQGRSLGLR
                     GLQERVQLVGGSVSIVSAPGAGTEVTLSLPMKSDPVPVFKEERR"
     gene            5365..5721
                     /locus_tag="VFR82_13195"
     CDS             5365..5721
                     /locus_tag="VFR82_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU4502602.1"
                     /translation="MKYMCLVYVEETILNAMPKKERQSLSDESMAYCNNLIKMGQCLA
                     ASPLHPVETAATVRVRNGKASTTDGPFAETKEQLGGFFLLNVRDLNDAIRMASMFPAA
                     RYGSIEVRPMKEDGCA"
     gene            5794..>6268
                     /locus_tag="VFR82_13200"
     CDS             5794..>6268
                     /locus_tag="VFR82_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEU4502603.1"
                     /translation="MSRVRVLVGTKKGAFILTADGKRKQWDINGPHFAGWEMYHLKGS
                     PADPNRLYASQTSSWFGQIIQRSDDGGKTWETPGGEKMPGAYDPPGGLSNKFVYDTSS
                     ESGKPLTTHQWYDGTQRPWEFKRVWHLEPSLTEPDTVYAGVEDAALFRSTDGGKTW"
BASE COUNT         1374 a         1872 c         1743 g         1279 t
ORIGIN      
        1 gtacagcgtg ctcggagttg atgcaacctg ggatctggct tactgggaaa gggggcaggg
       61 cctcggtgtg cagtcgctac cacaagctcc tgccaaggca aaacaccaca ggatcgacga
      121 ggagtggtcc gaaggcgcgg gggcttgcac tccgctacag gtgatgggtc agtgcataac
      181 gaataagggc cgacgtcgtc ttcaattgga gcttcgacag cagtcgcatt cggtaggtac
      241 ttaccgtttt cacgctcaga tcgagggcca ccgcaatttc agtgaccgat ttcccgccac
      301 cgatcatcga gagcacttgc agttctcgat ccgacagcaa cgtgtgaagc ggcttgcctc
      361 cagactggat gggcatggct tcgagccgct gcgccaatga aggtgtcaca tactgatgtc
      421 ctgcgaggac ggtcttaatc gctgtatcaa gttcttgtgg tgccgtgtcc ttggtcacat
      481 acgcggacgc cccgcttttc aacgcgcgaa cagcatattg ctcctcagga tgaaaactga
      541 gtacgacgat gggtagagcg gggcggagtg tttttatttt tttgaggaca tcgagtccat
      601 tggtatccgg cagatttata tcgagcacca cgagatccca ggcggcctgc tgtaccttgg
      661 aaagcgcctc ctgaccagtt ccggcctccg tcacgtcgag ttgatggaag gcgtccagca
      721 gcatctgcct gagtccgact ctgacgatgg catgatcgtc aacgagaagt atcttgagct
      781 tgacgccttg ctgatccggt aattcaatcg actgcgactg cgggagcgaa gcgggttggt
      841 tggtcaacat tagggtggat ttccatgcat gatcaatagg actgtctcgg gcgacactgt
      901 agcgaaattc gactagaaag tgaacaacac aatttgcgtg taagttatct cctacagcgt
      961 ttcgtgtagg ggctcacgtg ggacattcag cgccgttctt ctttgaacac tggtacaggg
     1021 tcgctcttca taggcaacga caatgtgact tccgtgcctg cgccgggagc actcacaatt
     1081 gaaacgctac cgccgacgag ttggactcgt tcctgcagcc cccgcagacc cagcgagcgg
     1141 ccctgtttcc actggtccgg cgtgaaccca cgtccgtcat ccccaatggt caacaccatc
     1201 tgaccttctc ccgccgtcag tcgcattgag gcctttgatg cctgggcatg ccgcatgacg
     1261 ttcgtcagga gctcctgggc cccgcgaaac acggtggtgg ccaatgccaa atcaggggac
     1321 acatcccgag cggatgaatc aatcatcaga tcgcacggcc agcccgtacg ttcatgaaac
     1381 gaggcggcga gccattccaa cgccgcaacc aggccgaggt cgtctaatac gccaggcctc
     1441 aacgacgcgg ccgttgcccg aacggacttg atcagtccgt caatagcgtg agccatgccc
     1501 atggtcttgg ctttgatgcc ggcgacatca ggcacctttt tcacacgaga catttccttg
     1561 gtcagccagg ccacatcaaa cttcaagccg gtaagtgcct ggccgaactc atcgtgcaac
     1621 tcgcgcgcca agcgccgtcg ctcaatctcc tccgcttcag tcaatcggcc agtcaggtct
     1681 cgaagctgct cgtaggcctc cttcagagcc cgttcggcct gtttgcgctc gctgatgtca
     1741 cggagaaacg cgctaaacac tttccggcct tcgatcgtca aggcggtgac ggagagttca
     1801 atgggaaatt cgctcccatc cttatgcagc gcagaaagtt cgacgcgcct attgaggatt
     1861 cgcccgtgac ccgacgacag aaagtgctgc agtccattgc tgtgagcctc gcggtaacga
     1921 gtcggcagga tcgtatcgct caacaatttg ccgctgattt cacgttcctg ataaccgaag
     1981 attgccgccg cttgcgaatt ccaaccggtt atgattcccc gttcatccat ggtgacgacg
     2041 gcatccaatg ccgactcgac gatggcccgc gtccgtgctt cgctcgcctg caatgccgcc
     2101 acagcttgct tccgcgcaat ggacgccgca aggccgttgc agacggactc aagcgtttgg
     2161 cggataggct ccgaaatgat ctcgcagctg aaaaggccca ggacgccaaa cacccggcct
     2221 tcgaccatca aggcaaatcc ggcaaacgca tggagtccat gttcacgcat ccactccttg
     2281 ttcggcaatc gatcgtcgtg cagcacgtca ttggtgaaaa gtgccccgtc gccttgagcg
     2341 atgcggccga tcttcagcgc tccgaggggg acacgacgat attccccatt cagattagtc
     2401 gacaaccccg aactcgcatg caaatgcagg cacgtggtgc ggtcgagaca tgacgacgcc
     2461 ttgtaacaat ccgcgcagag atctccgggc tcgagcagcc acacgcgggt gaatgctgcg
     2521 cccaaatgct ccacggcggc atccgtgcag cgttgcagca ggcagtccag cggctcgtca
     2581 tggttcaaca gcagactgac ctcagcggca aaggcggcac atttggcacg ctcctgtagc
     2641 gcctgttcgg ccatttttcg cgcggtgatg tcgcgggcga tggcctccac gccgacgagt
     2701 tgcccctcca ccagacgcaa tcgcacatgc tggccaaacc agacctcctc cgctgtcttg
     2761 gtcatggcag gaaactcata gtaggtgctg ggcacttgcg ccaacagctg gtctttgtaa
     2821 aattgctggg cccgatcccg agcatcctcg cgaatcagtt cgaggtagtg cttacccagc
     2881 aattcctgct cctcgtagcc catgagggcg caggcagcag cattaacaaa cgtaaaccgg
     2941 ccgtctactc cggccgtata aatgatgtcg gtcgcttgag acacaagaga actgtatcgt
     3001 gcttcggatt cgcctaacgc ttcttccgtc cgcttgcggg cggtcatgtc gatttcaatg
     3061 ccggccattc gtaccgccgc acccagcgcg ctgcgctcga tcacgcgtcc cctgctcaag
     3121 atccaggtcc attcattggc acgcgtttgc acccgcatct cggcctcaaa ttcgggtgtt
     3181 tcgccgcgaa gacaggcatt gagattcatt tcgaccatcg aacgatcgtc cgcatgaatc
     3241 ccgttcaaaa gggaagagac gtgcggcgcc atttcatgga cggcaaaccc tctgagctgc
     3301 gcccaccgct cattgaacga cacagctccg gtggcgatct gccaatccca cgaagccaaa
     3361 cccgcgccgt ggaacgccat ttcgagccgc tcctgactgg cccgcagcgc agccgtacgc
     3421 tcggccaccc gccgctcaag ctcgtggtgg gctcgttgca aggcttcttc cgctcgtttg
     3481 cgttcggtga tgtcatcggc gatgccggcg atccgacaga ccgatccgga actatcatgg
     3541 atgggaaagg cccgatcacg tatccaccgc atcgagccgt caggcctgat gatgcgatat
     3601 tcctcatcgt aggtcgccgt catttgtttg gcgagcgcgg cctccaagac acgttgccga
     3661 tcatcggggt gaatcgcctc cagccaggat cgcggcgaac gataaagact ctcgcatgag
     3721 cgcccccaga tggactcata tcccgggctc acatagagga cggcattctt cgccgtatcg
     3781 ctcagccaaa acacctcttg gatatgctcg gtgacctgcc ggaagtgctc ctcagtctcc
     3841 cgcaacgctg tctcgatttt attgcgttct gtgatatcaa ccgccacccc tccaatcagc
     3901 gccagattcc cttcctcgtc gacgattgga aacttgctca ccaggctttg atgtaatcca
     3961 tccttgtagg gggcggtctc gactgccacc accgccttgg cggtggccct cacctgtgca
     4021 tcgttggctt ggaactgagc ggcagtgtgg tctggcagga tttcacaatc ggtcttttct
     4081 cgccaatcct ccagacggac gccgaaggca tgggtaaagg catcattgaa atacagatat
     4141 cggccctgta tgtccttaat ccaagcaaga gcaggcaggt gggtcatgaa tgcagcgaag
     4201 aggcgatcgc tttgccgcct cgcctgatca gcctcattca tcctgcgggc ggttccccag
     4261 aggagagcgg ccaaggtcac cgtggctgtt gtggcgaaga tggccacacc catgtccgtg
     4321 ccataccacc cttggcgctc cccatacagc cgaatccatc cgagcgccgg caggatcacg
     4381 aagacgccgg gtagcacgcg tctcagcatg tacccgccgg cttgattact gttgatcacg
     4441 gccatgagtc ccttggccgg ctccaacgcc aatgcgcctg cgccaaccag catgagggtc
     4501 agcgcggtat gtaaggccat aggcgtggac ggaccgaccc cgtaaaggaa tcgctcctga
     4561 tagagatacc cgagaaacgc caggaacccc aaaaaggttg cacccaaaga gaatccgtcg
     4621 ctcacaccca caagacggcc ctgtgagagt atcttcaagg caagcgccag accaagcaac
     4681 agaaagcaga cggccgtgtt gatgcccatc cgccctggta ttgatggccc gaccgcttca
     4741 agcggctcat aaagcaccca ctgatcgacg ctcagatcga ccataagaag atattcaaga
     4801 cccgtggcca ggccgatgat cgcggtcacc gcagcagctc cgcccgcaac gaaccgcacg
     4861 tgctgtcgag gcgttctcat tgatgcggcc aacaagctga ttcctgaaaa gacaaacgcg
     4921 atcgctgcat tgggcatcat ggggatcaaa ccgggagcta ggcttttgag atccgtgctg
     4981 ttcacggccc agccaaagag gacgagcgcg cccacttcaa gcgacaggag gcccgccaat
     5041 ggagggagag ggtgtacgaa acgcgaacga agtgccgtca agagagtcac atcaactcgc
     5101 gtatcagagg ttggcgaagg atgaaaggaa acatgaggat tgtcaagata agggtccttc
     5161 tgtaggtgcc tatatgacaa ccgctgaaca tctgcggcaa aattcgggct cctccgggtc
     5221 accctatggc catgttgcga ttcacttcgc gcatgaatca ccataaggct cgctctggag
     5281 aaagactcgg ggcaccctgt cgattttgac gacccaagac gactatgttc tgtcagggca
     5341 ttgatcacca ccccggaggg agtcatgaaa tatatgtgtc tagtctatgt cgaggagacg
     5401 attctcaacg cgatgccgaa gaaggagcgg caatcgctct cggatgaatc gatggcctac
     5461 tgtaacaatc tgatcaagat gggacagtgt cttgctgcct cgcccctcca ccccgtagag
     5521 actgccgcaa cggtgcgcgt tcgcaacggt aaggcgtcca ccaccgacgg cccctttgcc
     5581 gagacaaagg agcagctggg tggatttttt ctgctcaatg tgcgggatct gaacgatgcg
     5641 attcggatgg catcgatgtt tcccgcggcc cgctatggca gcattgaggt gcggcctatg
     5701 aaagaggacg gctgtgcctg aacggcctga cagtgccact ccattgaatg tgtcgtcgtc
     5761 atcatcctct acaggaaggg aaaggagtca tctatgagcc gtgtacgggt cctggtggga
     5821 acaaagaaag gcgcatttat attgacggcg gacggcaagc gaaaacagtg ggacatcaac
     5881 ggcccccatt ttgccggttg ggaaatgtac cacctcaaag gatcgcctgc cgatccaaat
     5941 cggttgtatg cctcgcagac cagcagctgg ttcgggcaga tcattcaacg ctccgatgac
     6001 ggcggcaaga catgggagac gcccggcggt gagaagatgc ctggtgcgta tgaccctccc
     6061 gggggactca gtaacaagtt tgtctacgac acatcctctg aatccggcaa acctcttaca
     6121 acccaccagt ggtatgacgg aacccaacgt ccatgggaat tcaagcgggt gtggcacctc
     6181 gaaccatcgc tgaccgagcc tgacaccgtc tacgctgggg ttgaggacgc ggccttgttt
     6241 cgctcgaccg acggagggaa gacgtggc
//
LOCUS       DASTQY010000407         3076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1266941, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000407 DASTQY010000000
VERSION     DASTQY010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266941"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFR82_13205"
     CDS             <1..333
                     /locus_tag="VFR82_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502604.1"
                     /translation="MIVALILLVLLTSGCIMIDTDLQLPDETVKPMLTGEDCVGIYFG
                     IGIGTVRMTEAMKTYHNVQQERQTPLGPEKGIVRGALLIRRIHSVVLKDVGTLGFGRR
                     CLEIKGEP"
     gene            467..964
                     /locus_tag="VFR82_13210"
     CDS             467..964
                     /locus_tag="VFR82_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502605.1"
                     /translation="MRYGLIVVFIILSQLDASAARAGRVEDRESLRRLPGVEVIVEKF
                     LSEEEVAGFSQDGIRKEVELVLQSNGVRLLSASERLKTASAPFLYVKVNPIKDPSAGT
                     YCLAIEVELHQAVSLLNRPEQQMSARTWNRAQVAIIGEQVTGRVTEAIEPLIKQFAND
                     FLTVN"
     gene            1532..2572
                     /locus_tag="VFR82_13215"
     CDS             1532..2572
                     /locus_tag="VFR82_13215"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEU4502606.1"
                     /translation="MIILLLFLALFSVAELAYRVRVDGGVKKATRNLLGYVRDVPYSN
                     LGTGHWVIYDDQLGYRLNPNRSGINSLSVRHGEIINPKPPGKYRVLVLGDSIPWDKLG
                     FVDYLTELLSKEADIEVINAAVPGYTAYQEVIFFKSYLQQSEPDLVIWTYCLNDNHKF
                     LHRFDEQAKMLWTNEAIESLTPTSSLTKLVSRSYILSELRLRISTIATIQQACRFPWE
                     CVPDFNIAWKDEPWTQYEAYLVEMERLIEQIHSRLSIVIFPYEPQLEQHGRVQDQSIE
                     YLSKPQRQMNALCRKYDVLCMDLFPAFQEKYGNGIKLFRDGIHLNKEGHELTGALIYT
                     FLREKDLVPVSR"
BASE COUNT          780 a          768 c          808 g          720 t
ORIGIN      
        1 atgatcgtcg cccttatcct tcttgtcttg ctcacgagtg gctgcattat gatcgacacc
       61 gacctacagt tgccggatga gaccgtgaaa cccatgctga ccggcgaaga ttgcgtcggc
      121 atctactttg gaattggtat cggcaccgtg cggatgaccg aggccatgaa gacgtatcac
      181 aacgtccaac aagaacgcca gacacctttg ggtcccgaga agggcattgt tcgcggcgcg
      241 ctgctgatcc gccgcattca ctcggttgtc ttgaaggatg tggggacgct cggcttcggg
      301 cgacggtgcc tcgaaatcaa gggtgaaccg tgagatggtc cggagaaaca cccacccgcc
      361 ctggttggta ttggtacggg cccaccccta aagcctcgcc ctcaactgat aggtcggcca
      421 aaaggttggt cgcaggccat cgagaaagca gcgcaaagga gatgtcatgc ggtatggact
      481 gattgtggtt tttataattc taagtcaact cgacgccagc gcggcgcgtg cagggcgggt
      541 tgaggaccgc gaatcactac ggaggctccc cggggtagag gtaatcgtag agaaattctt
      601 gtcggaggaa gaagttgctg gcttttcaca ggatgggatc cgcaaagagg tcgaactggt
      661 tcttcagtcg aacggggtaa gacttctcag tgcatcagaa cgactcaaga ccgcctcggc
      721 tccctttctt tacgtgaagg tcaatcccat caaagacccc agtgcaggaa cctactgttt
      781 agccatagaa gtcgaacttc accaggcagt gtcattgctg aacagaccgg aacagcaaat
      841 gtccgcgcgg acatggaaca gagcgcaggt tgcaataatc ggcgagcaag taacaggtcg
      901 agttaccgag gcgattgagc ccttaataaa gcaatttgcc aatgactttc ttactgtcaa
      961 ttagcacata gaacgaagga gcgcgctcta atgatcgccg ccatctcctc ccggaaatca
     1021 actgaacaga tcgggccaac aatgatgaga aatccgtcac gcggcagatt gaacatgcaa
     1081 aactatatgc cacaaaaacg ggcaactacg ctgctctact gcctgagacg ctccctccgc
     1141 tcaattcacc caaagatggc tccgttgaat ctagtgtccc caacggggat tagagggatt
     1201 ttgtaacggt cctgatagca catggggtat tcctttccag gggtttgtga atgccacatg
     1261 acctgcggtt tacgagtgtc acagaagaca gggcgccgcc gtcagcggga agtaaggcca
     1321 ggaagctctt gaaacgacca ttccgaaaaa cgaaacctag gcggtgtggt agccagctct
     1381 ccttccattc agcgagcaca caccccccgc gtcttcgttt tcttattgcc tatgacacat
     1441 gagcaaccct accatcgcct cctctgagag atccaactgg tacatgaaca atcggtttcc
     1501 cagcacagtt cgccggtctt gctatgtggg catgatcatc ctactgcttt ttttggccct
     1561 gttctcagtg gctgaattgg cgtacagggt gcgtgtcgat ggcggcgtga aaaaggcaac
     1621 tcgcaacctg cttggatatg tgcgcgatgt gccgtactcc aacttgggca caggccattg
     1681 ggtcatctat gatgatcagt tgggataccg actcaatccg aatcgatcag gcatcaattc
     1741 gctctctgtc cggcacgggg aaattatcaa tcccaagcct cccggcaaat atagagtgtt
     1801 ggtgttggga gattccattc catgggacaa actcgggttt gtggattatt tgactgaact
     1861 gctgagtaaa gaggcggaca tcgaggttat taatgctgcg gtgcctggat acacggctta
     1921 tcaagaggtg atatttttca aaagctatct acagcaatcg gagccagacc tcgtcatttg
     1981 gacctattgt ttgaatgata accataaatt tcttcatcgc tttgacgagc aggcgaaaat
     2041 gctttggacg aacgaggcaa ttgaaagcct gacacccaca tcttccttga caaagctggt
     2101 gagccgttcc tacatcctga gcgagttgag actcaggatc agtacaatag caacaataca
     2161 gcaagcatgt cgcttccctt gggagtgcgt cccggacttt aacattgcct ggaaagatga
     2221 accgtggact cagtatgagg cgtatctcgt tgagatggaa cgccttatag agcaaataca
     2281 tagccgctta tctattgtta tttttccgta tgagcctcag ctcgaacaac acggtcgcgt
     2341 ccaagatcaa agcatagaat atctatcgaa gccacagagg cagatgaatg ccttgtgccg
     2401 gaaatatgat gtgctctgta tggatttgtt ccccgccttt caggagaagt atgggaacgg
     2461 catcaaacta ttcagggatg gtattcatct aaataaggag ggacatgaat tgacgggggc
     2521 gctaatctac accttccttc gtgagaaaga tctcgtgccg gtcagtcgtt gagagaaggt
     2581 acacctcgct tcagctttcg gtctgcctcc ctttcctgac ggatttgaca agtgtcttgc
     2641 tgttaacgcg atccagtttg ggaaaaccct actgaagtat tgcggaactt gcgacggctg
     2701 ttaaatccca gagggagaat cgtgttggct cttcaacctc gttttcaggg tgccataaac
     2761 gaggatacta ctcaggctgg tcatgacatg ctagagaagt taaaagaagc aggcttctca
     2821 caggtccagc tagagatgaa acccatgaag ccagtctccg ttgcctatgc ggtgggcatc
     2881 aatgacgtgt gatgcagtgt atgcgggcag catgtctcat caagcacgcg cactccaata
     2941 ccgattgcga tataaaatca gtcgatctag cctacaaccg attgcacgca atcagacccg
     3001 cgcggatgtg ccattcaagg aaaggaagtg cgtatggcta agctcgtgtt cggaatgaac
     3061 cagtccctgg acggct
//
LOCUS       DASTQY010000408         3390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1619162, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000408 DASTQY010000000
VERSION     DASTQY010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619162"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /gene="lpxC"
                     /locus_tag="VFR82_13220"
     CDS             <1..713
                     /gene="lpxC"
                     /locus_tag="VFR82_13220"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEU4502607.1"
                     /translation="EHVLAALAGLNVDNAYVDIDASEAPVMDGSSAPFVRLIESAGVV
                     SQNRLRSFLKITRPLEIVDGSRRIRIEPASTPRITYTIHYDHPLIQTQTYAYEHSSRA
                     FASDIAGARTFGFLQEVEALWARGLGRGGNLENTVVLSQDGILNESGLRFTDEFVRHK
                     VLDLIGDFSLLGLPFIGHLIADRSGHALHTRLVKQILDHPECWVLLNADHSAIQAEAS
                     LSLSTPAPRSSAVALHAS"
     gene            complement(918..1421)
                     /locus_tag="VFR82_13225"
     CDS             complement(918..1421)
                     /locus_tag="VFR82_13225"
                     /inference="COORDINATES: protein motif:HMM:NF016924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU4502608.1"
                     /translation="MEELTDILVGLEQRISAYRNRLQDLEKKRRRLDDEIATIKKYLE
                     LADTLHRVEADKTKLFNAASLLIADEKGSRILPVEVTDQSREILLGRSKYAGKSVPEA
                     AYQVLREANRAMHAKELVQRLLEGGLHIKGKTPLTSVATSLKRDKRFRKVGPNTFEAV
                     EDVLSHA"
     gene            1555..2367
                     /locus_tag="VFR82_13230"
     CDS             1555..2367
                     /locus_tag="VFR82_13230"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU4502609.1"
                     /translation="MPPQDEAVTTARLLSVAVDACKSAGGLLLDYARSGFQVEHKNAI
                     DLVTDADRGAEQCIVDHIRAHFPTHSTLAEERGIDAPLTSRYQWIIDPLDGTTNFAHR
                     FPVYAVSIGVEWNGRGVVGVVYDPTRDELFTAEAGHGAYLNGHPMTVSKTTQLDGALM
                     VTGFAYDIRDTADNNLNHFARFALKAQGVRRTGSAALDLCYVAAGRFDGFWEVKLSPW
                     DMAAGVVILRAAGGRVTDLRGHEHSIYQSEVVASNGRIHDEMLAVISESLSS"
     gene            2459..2740
                     /locus_tag="VFR82_13235"
     CDS             2459..2740
                     /locus_tag="VFR82_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502610.1"
                     /translation="MASTIPPSGSSKGNPQSAGAESTSWDVELLHVLVSRNGSQVEAQ
                     WSIHPQLKQDLLPHEWQEVTDLMGKVSGIVGNRFSQVLAQADPETPTNA"
     gene            2799..3131
                     /locus_tag="VFR82_13240"
     CDS             2799..3131
                     /locus_tag="VFR82_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HEU4502611.1"
                     /translation="MDTYYHPHDLGKFSDMEKGNKPLWDKFMSYYSAVFAEGALTERE
                     KALIALAVAHTVQCPYCIDAYTQSSLEKGSNVEEMTEAIHVACAIRGGASLVHGVQMR
                     NVAAKLSM"
     gene            complement(3160..>3390)
                     /locus_tag="VFR82_13245"
     CDS             complement(3160..>3390)
                     /locus_tag="VFR82_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM protein 1"
                     /protein_id="HEU4502612.1"
                     /translation="NPKAPTRRRYDGVAALVIEMCNEAPKTVEQLVAVAAHPGTSQAE
                     IEPILADLTSRRVLYQERGRYFTLAIPEHPYL"
BASE COUNT          727 a          951 c          925 g          787 t
ORIGIN      
        1 ttgagcacgt cttagcggcg ctcgcaggct tgaacgtcga taatgcctat gtcgatatag
       61 acgccagcga agcgccggtc atggacggta gttcagcccc ctttgtccga ttgattgaat
      121 cggcaggtgt ggtgtctcag aaccgactcc gatccttttt gaagattaca agacccctcg
      181 agatcgtcga cggatcgcga cgcatccgaa ttgaaccagc ttccaccccg cgaattacct
      241 atacgattca ttacgaccat cccttgattc aaacgcaaac ctacgcgtat gaacactcct
      301 cccgtgcgtt cgcgtctgat atcgccggag ccaggacgtt tggatttttg caagaagtag
      361 aagcgctgtg ggcccggggg ttaggtcgcg gggggaatct cgagaatacc gtcgtccttt
      421 cgcaggacgg gattctcaat gaatccggcc ttcgtttcac cgacgaattc gtccgtcaca
      481 aggtgctaga tttgattggt gacttctcgc ttctggggct gcccttcatt ggccatttga
      541 ttgcggaccg ctcaggacat gccctccata cgcgtctggt gaaacagatt ctcgatcacc
      601 cagaatgttg ggtgcttttg aatgccgacc attcagccat tcaagccgaa gcgagtctgt
      661 ctctctccac acctgccccg agatcttcag ccgtcgccct gcacgcgtcc tagatcgtta
      721 ttcactcgtg cgttcttttt gatctctaac ggaatctgtt tcggcagatc aatcgataac
      781 gtcgggagcg agtggcctcg cccccgacgc tagaatcgag gcgggagtta cttctttttc
      841 ttcttcgctg ctttcttcgt cgccaagacg ctcacctccc ttctcaacac tcaggagcct
      901 ttacaaggcc gtcgttttta cgcatggctc aacacatctt caacggcctc gaaggtgttg
      961 gggccgactt tgcgaaagcg cttatcacgc tttagcgatg tcgccacaga cgtgagcggc
     1021 gttttgccct tgatgtgcaa accgccctca agcagccgct gaacgagttc cttggcatgc
     1081 atggcccggt ttgcttcacg caacacctga taggccgctt ccggtacgct cttcccggca
     1141 tatttactcc gtcctaaaag gatttcccgt gattgatccg ttacttcgac gggaaggata
     1201 cgactgccct tctcgtctgc gatgagcaat gacgccgcgt tgaacaactt cgttttgtct
     1261 gcctccaccc gatggagggt gtccgccagt tccaggtatt tcttgatggt cgcgatctcg
     1321 tcgtccaggc gacgacgttt cttttcgagg tcttgaagtc ggttcctgta ggcgctgata
     1381 cgctgttcaa ggccgaccaa aatatcggtg agttcttcca aggaaagctc cctcggcaag
     1441 catgcttgct ttatatgctt gcttgcataa gaagtcaatg cccggcgaag aagaaaaggc
     1501 ttgtgtctat gcaactgtaa actgaatggt ggaacgcatg ttaaaatggg ggcaatgcca
     1561 cctcaagacg aggctgtcac gaccgcacgc ctgctctctg ttgccgtaga cgcatgcaaa
     1621 tcggcgggag gcctgctgct ggactatgcc cggtccggat ttcaggtaga acacaaaaac
     1681 gcgattgacc ttgtgactga tgccgaccgc ggcgccgaac aatgcatcgt agaccatatt
     1741 cgcgcgcatt tcccgaccca cagcactctc gccgaagagc gaggaatcga cgcacctctc
     1801 acatcgcgct accagtggat catcgatcca ttggacggta cgactaactt cgcccatcga
     1861 ttcccggtct atgcagtttc aatcggggtt gaatggaacg gtcgaggagt cgtgggggtg
     1921 gtgtacgatc ctactcgaga cgaactattc accgcggaag ccggacacgg cgcgtaccta
     1981 aacggccacc cgatgacggt ttcgaagaca acccaattgg acggtgccct gatggtcact
     2041 gggtttgctt atgacattcg cgacacggcc gataataacc tgaaccactt tgcacggttc
     2101 gccttgaagg cacagggtgt gcggcgaaca gggagcgcag cgctggatct gtgttatgta
     2161 gccgcgggcc gattcgatgg tttttgggag gtgaagttga gcccctggga catggcggcg
     2221 ggcgtcgtca tccttcgagc agctggcgga cgcgttacag accttcgagg tcacgagcac
     2281 tcgatctacc agtccgaggt cgtggcgagt aacggtcgga ttcacgatga aatgctagcc
     2341 gtgatcagcg aaagcctctc ttcctaacct gtttcgaagg ctctggtcgc agatgatttg
     2401 gcatgtacag cgcgatttgc cctacgttat gatgggccca taacagaagg agtcgacgat
     2461 ggccagcact atccctccat caggatcctc gaagggcaat ccacaatcgg cgggagcgga
     2521 atcgacctcg tgggacgtcg agctattgca cgtcttggtc agccggaacg gttcgcaagt
     2581 cgaagctcaa tggtcgatac atcctcaact gaagcaggat ctcttgccgc atgagtggca
     2641 ggaagtcacc gatctgatgg gaaaggtatc cggcatcgtc ggaaaccgct tctcacaggt
     2701 gctggctcag gccgatcctg agactccgac caacgcctga cgcggaatca atccaagcca
     2761 atcacttcta tcattcactt ctatcattgg agcgccgcat ggacacctat taccacccgc
     2821 acgatctcgg caagtttagc gacatggaaa agggcaacaa gccactatgg gacaagttca
     2881 tgagttacta cagcgcggtg tttgccgagg gcgcgctgac cgagcgcgaa aaggcgctga
     2941 tcgcgctggc agtcgcccac accgtgcaat gcccctattg catagatgcg tacacgcagt
     3001 cgtcactcga gaaggggtca aacgtagaag aaatgactga agccattcac gtagcctgcg
     3061 cgatacgagg tggggcatct cttgttcacg gggtacaaat gcgcaacgtg gccgcgaagc
     3121 tgtcaatgtg aggaaggatt cagcatgtct gccctctccc tagagatagg gatgttccgg
     3181 aatggccaat gtgaagtacc tcccccgttc ttgatacaag actcgtctcg aggtgagatc
     3241 ggctaagatc ggttctatct cagcttggct tgtgcccggg tgggcagcca ccgccacaag
     3301 ttgctccaca gtcttgggtg cctcattgca catctctatg accagggcgg ccacgccgtc
     3361 gtagcgccgc cgcgtgggcg cttttggatt
//
LOCUS       DASTQY010000409         3740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1691190, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000409 DASTQY010000000
VERSION     DASTQY010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691190"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VFR82_13250"
     CDS             <1..897
                     /locus_tag="VFR82_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU4502613.1"
                     /translation="TIGSKEGLAHLSLAMIGPGDVVLTPTPTYPIHMYSFVIAGGEVR
                     GIELRQDSDFFDDLQRVYRQTFPRPRILVINFPHNPTTAIVDLEFFKKIVAFAKEHHV
                     IVIHDLAYADLVFDGYRAPSFLQVPGAKDVGVEFYTLSKAYNMPGWRVGFCVGNREVV
                     GALAKIKSYLDYGIFQPLQIASVIALNGPQDCVKETVQRYQKRRDVLVGGLNRIGWNV
                     NKPLATMFVWARIPLPYRHMGSLEFSKLLLREAKVAVSPGVGFGEGGDEYVRFALVEN
                     EHRTRQAVRGIRKALKLEGGEA"
     gene            894..2207
                     /locus_tag="VFR82_13255"
     CDS             894..2207
                     /locus_tag="VFR82_13255"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU4502614.1"
                     /translation="MISRVGVGIVGFGTVGSGVAKILLENAALIRRRVGVPIELVRIA
                     DLDTTRDRGMALPAGVLVKDAKQILADPAIDIVVELVGGYDFAKRLILDAIDVGKSVV
                     TANKALLALHGEEIFAAASRKGIDIGFEASVGGGIPVIHALKEGLAANSIQSIYGIIN
                     GTSNYILSRMTNEGKGFGETLVEAQKAGYAEADPAFDVAGTDSAHKLAILVNLGFGTP
                     VNFKDLYTEGITHITEYDIAYAREFGYTIKLLAIAKLVDGEVEARVHPTMLGDESPLT
                     KIEGVYNAIQLVGDAVGDVVLSGRGAGDLAAGSAVVSDIIAGARNLLKGAAGRVPAAS
                     FQPNERRPLRMRRMEEIRSLYYLRFMVTDRPGVLAQIAGELGRYGISIASMLQKGRRE
                     GQTVPIVIKTHTATERDVQTALSHINRLSFMSEPTTLIRIEGKDE"
     gene            2211..3269
                     /gene="thrC"
                     /locus_tag="VFR82_13260"
     CDS             2211..3269
                     /gene="thrC"
                     /locus_tag="VFR82_13260"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU4502615.1"
                     /translation="MTRWRGIIAEYRKFLPVNDQTPIVTLDEGNTPLIQAARLAKKIA
                     PGIDLYLKFEGANPTGSFKDRGMTLAISKAVENGARAVICASTGNTSASAAAYGARAG
                     LAVYVLIPAGKIAMGKLSQAMMHQATVIQVDGNFDQALSIVKTLSAAHHIELVNSLNP
                     FRIEGQKTAAMEVCDQLGDAPAVHVLPVGNAGNITAYWKGYKEYRAANQSRSLPRMIG
                     FQAAGAAPIVLGRVVENPQTVATAIRIGNPASWQAAVDAVKESSGAIDSVTDEEILQA
                     YVAVAGTEGVFCEPASAASVAGVMKLSRQGRLREGETVVCTLTGHGLKDADTAISVSV
                     KPQTVNATTEEVARLLKV"
     gene            3293..>3740
                     /locus_tag="VFR82_13265"
     CDS             3293..>3740
                     /locus_tag="VFR82_13265"
                     /inference="COORDINATES: protein motif:HMM:NF021646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502616.1"
                     /translation="MKYVILHAEGMSDHPRQELAGKTPLQAASTPHLDRLAEQSELGL
                     LTVALDNGRHGSGLTGTSILGYEPKKYYQGPGPLEAASLGVTVGEHDVVYRCTMVTLK
                     SEGPSGAKPSASDIKKLGPHIVMDDATAGLITSEEARELIDAVNEQL"
BASE COUNT          828 a         1006 c         1140 g          766 t
ORIGIN      
        1 acgatcggat ccaaagaagg tctcgcccat ttgtcgctgg ccatgatcgg tccgggggac
       61 gttgtgctga cgccgacacc gacctatccc atccacatgt acagctttgt gattgccgga
      121 ggggaagtgc gcggaattga attgaggcag gatagcgatt tctttgatga cctccaacgc
      181 gtctaccgcc agacctttcc ccggccgagg attctcgtca tcaactttcc gcataatccg
      241 acgacggcca tcgtggacct cgaattcttc aagaagatcg tcgcgtttgc caaggaacac
      301 cacgtcattg tcattcacga cctcgcttac gcggatcttg tgtttgacgg ttatcgggcg
      361 ccaagctttc tacaagtgcc tggtgccaaa gacgtgggcg tcgagttcta taccctgtcg
      421 aaagcctaca atatgccggg atggcgggtc ggattttgtg tcggcaatcg ggaggtcgta
      481 ggggcgctag cgaaaatcaa gagttatcta gactatggca ttttccagcc gctgcagatc
      541 gccagcgtca ttgccttgaa cggtccgcag gactgtgtga aggagacggt tcaacgctat
      601 caaaaacgcc gtgatgttct tgtcggagga ctaaaccgca tcggctggaa cgtgaacaag
      661 ccgctcgcga ctatgtttgt gtgggcgagg attccactgc catatcgcca tatgggatca
      721 ctcgaatttt ccaagttact gctgcgagag gccaaagtcg cggtctcacc cggcgttggc
      781 ttcggagaag gcggcgatga atacgtacgc ttcgcgcttg ttgaaaatga gcaccggaca
      841 agacaagccg ttcgtgggat caggaaggca ttgaagctcg aaggaggaga ggcgtgatct
      901 cgcgggtggg cgttggcatt gtcggctttg gcaccgtagg ttcgggcgtg gccaaaatac
      961 tccttgagaa tgcagccctc attcgccgcc gagtcggcgt gccgatcgag ttggttcgaa
     1021 tcgccgatct ggacacgacc cgcgatcgcg gcatggccct gccggccggg gtcttggtga
     1081 aggacgctaa gcagatattg gccgatcccg ccatcgacat cgtagttgaa ttggtgggag
     1141 gatacgattt cgccaagcgt ctcattcttg atgccatcga cgttgggaaa agcgtggtga
     1201 ccgcaaacaa ggctcttctg gcgcttcacg gggaagagat ctttgcggct gcctcccgga
     1261 aaggtatcga cattggattc gaggccagcg tcggaggcgg aattcccgtc attcatgcgc
     1321 tcaaggaagg gttggcggcg aactcgatcc aatccatcta tggcatcatc aacggcacgt
     1381 cgaattatat tctctcccgc atgacgaacg aggggaaggg gtttggcgaa acgttggtgg
     1441 aggcgcagaa agcgggatat gcggaggcgg atccggcgtt cgatgtggcg ggtacggata
     1501 gcgcacacaa actcgcgatt ctggtcaacc tggggttcgg cacgccggtc aacttcaagg
     1561 atctctacac cgaaggcatt actcacatca ccgagtacga catcgcctac gcgcgggaat
     1621 tcggctatac gatcaagctg ttggcgattg cgaagctcgt tgacggtgaa gtcgaggcgc
     1681 gcgtccatcc caccatgttg ggcgacgagt caccgttgac caaaattgaa ggcgtgtaca
     1741 acgccattca attggtgggc gatgccgtcg gcgacgtggt cttgagcgga cggggagcgg
     1801 gggatctggc ggcaggaagc gcagtggtca gcgatatcat cgcgggtgcc cgcaatctcc
     1861 tcaagggcgc ggcaggtcgc gtgccagcgg cttcctttca accgaatgag cggcggccac
     1921 ttcgtatgcg gcggatggag gaaatacggt cgctgtacta cttgcgtttc atggtcaccg
     1981 atcgtccggg cgtattggca caaatcgcag gtgagttggg acgatatggc atcagcatcg
     2041 cctcgatgtt gcagaaaggg cgtcgggagg gacagaccgt tcccattgtc atcaagactc
     2101 atacggcgac agagcgtgac gttcagaccg cgctcagtca catcaatcgg ctgtcgttca
     2161 tgtccgagcc caccactctc atccgcatcg agggcaagga cgaataagtc atgactcgct
     2221 ggcgcggcat cattgcagaa taccgaaaat tccttcccgt caacgatcag actcccatcg
     2281 tcacgcttga tgaaggcaat acgccgttaa ttcaggcggc caggcttgcc aagaagatcg
     2341 ccccaggaat cgacctgtat ttgaaatttg agggagcgaa tccgaccgga tcgttcaagg
     2401 acagaggtat gacgctggcg atttcaaagg ccgttgagaa tggcgcccgc gccgtcatct
     2461 gtgcctccac aggcaacacg tcagcatcag cggcggccta tggcgcccgg gccggattgg
     2521 cggtctatgt gctgatccct gccgggaaaa tcgccatggg aaaactctcg caagccatga
     2581 tgcaccaagc gacggtcatc caagtcgacg gaaacttcga tcaggccctc tcgatcgtga
     2641 aaacgctctc cgccgcacat cacatcgaac ttgtaaattc tctgaatcca tttcgtatcg
     2701 agggacaaaa gaccgcagcc atggaggtgt gcgatcaatt gggcgatgcc ccggcggtcc
     2761 atgtccttcc ggtcggaaat gccggcaata tcactgccta ttggaaaggc tacaaggaat
     2821 atcgtgcagc caatcaaagc cggagtctcc cgcggatgat cggatttcag gcagccgggg
     2881 ccgcacccat cgtgctcggt cgggtcgttg agaatcctca gaccgtggcg accgcaatcc
     2941 ggatcggcaa ccccgccagt tggcaggcag cggttgacgc ggtgaaggag tcatccgggg
     3001 cgatcgattc cgtgaccgac gaagaaatcc ttcaagccta tgttgcggta gccggtacgg
     3061 aaggagtgtt ttgcgaaccc gcctcggcgg cctccgtggc cggcgtgatg aaactgagcc
     3121 gtcaaggtcg gttgcgcgag ggcgagacgg tcgtatgcac gctcacgggt catggcttga
     3181 aagatgccga cacggccatc agcgtatcgg tcaaaccgca aaccgtcaac gcaacaacgg
     3241 aggaagtggc tcgacttctg aaggtgtaag gcacgatgcg agtgtgtggt cgatgaaata
     3301 tgtgattctg catgccgagg gaatgtccga ccatccccgg caggaactag ccggcaagac
     3361 tccacttcaa gccgcctcca ctccccacct tgatcgattg gccgagcaga gcgaactggg
     3421 tctcctcacg gtcgctctgg acaatggccg tcatggtagc gggctgactg ggacctccat
     3481 cctcgggtat gaacccaaaa agtactatca ggggccggga ccattggaag cggccagttt
     3541 aggtgtaaca gtaggcgagc acgatgtggt ctaccgctgt accatggtga ccttgaaatc
     3601 tgaaggtccg tcgggcgcca agcccagcgc gtcagatatc aagaagctag gtcctcacat
     3661 tgtcatggat gatgcgacgg cagggctgat tacgagcgag gaggcccgtg agctgattga
     3721 cgcggtcaat gagcagcttg
//
LOCUS       DASTQY010000410         4481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1097575, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000410 DASTQY010000000
VERSION     DASTQY010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097575"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VFR82_13270"
     CDS             complement(<1..817)
                     /locus_tag="VFR82_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU4502617.1"
                     /translation="MNAELRRLTKALIFAAEAHRNQRRKGAAQEPYLNHLVEVLDLVV
                     QASDGVDMDVALAALLHDVVEDTSTSYEDVAATFGDRVAKIVRENSDDMSLPKTERRR
                     ARIAAIPLKSREARLVKMADVISNLRAIAVSPPAGWPAERKLGYLEGCRHLVDAARGT
                     DAELERIFDQTAADVERTIREETSFSIEGREVAARHLDSAIGQAVHLVYLLNTENVTL
                     GASDVDRLCELIGRSFPAATVQPAEAIHDGQRRSILMVRIRTDSTEAVVELAQR"
     gene            complement(853..1503)
                     /locus_tag="VFR82_13275"
     CDS             complement(853..1503)
                     /locus_tag="VFR82_13275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEU4502618.1"
                     /translation="MDAQGIIAKRIAQELRPGMLVNLGIGIPTLVANHVPEGVSVFFQ
                     SENGLIGTGPIPEEGMTHPTLTDAAGKPVSALPGASTFDSAMSFGLIRGGHVDMTVLG
                     GLQVDEQGRLANWMIPGKMVPGMGGAMDLVTGAKRVVVAMQHTAKGKPKIVKQCTLPL
                     TSLRQVDLVVTDLAVIAFPERRATLLETAPGITVQDVVTATEAALVVSGHVPQMTI"
     gene            complement(1508..2155)
                     /locus_tag="VFR82_13280"
     CDS             complement(1508..2155)
                     /locus_tag="VFR82_13280"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEU4502619.1"
                     /translation="MKTIALEDAVAMIPDGASLMIGGFMAVGTPERLVDEIVRQGKRN
                     LTVIANDTAAPGRGIGKLVSAGLIARVIASHIGLNPETQQQMITGKMNIDLVPQGTLI
                     ERIRAGGFGLGGILTPTGVGTVVEEGKRNIEVDGKNFLLETPLRADFALIHSFLADYL
                     GNLSYALTARNFNPVMATAADTVIVTAEHIVPVGVIAPDHVVTPAPIVDYLVANE"
     gene            2287..2745
                     /locus_tag="VFR82_13285"
     CDS             2287..2745
                     /locus_tag="VFR82_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502620.1"
                     /translation="MVASAEKVSGLPSAKEMMEKLALAEAKKAEEAARKKAIVEGEKK
                     ALLDNLTKASGVSDDEALQRVKTIVERAVANGLTEVLVYRFPNQLCTDRGRAINQQEA
                     GWETTLTGLPKEMYEFWKRQLQPLGYKIRFQIVDFPGGMPGDVGITLRWA"
     gene            2882..>4481
                     /locus_tag="VFR82_13290"
     CDS             2882..>4481
                     /locus_tag="VFR82_13290"
                     /inference="COORDINATES: protein motif:HMM:NF022783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HEU4502621.1"
                     /translation="MNTQPHKAEFEPTSLLWPVDLAREAWAYGVDTLQRSILFLDVLR
                     ERGKGYEEHAAKLAPHVLKFECELVMDGRKLPRPVNYLLVRITPPAGVEIDERKRPFV
                     VVDPRAGHGPGIGGFKAQSEIGVAFQAGHPCYFIGFLPEPEPGQTISDIAYAEAAFLE
                     RVIALHPKTDGKPAVIGNCQAGWAVMIVAALRPELFGPIIVAGSPLSYWAGMRGQYPM
                     RYTAGMLGGSWLTSLMGDLGNGKFDGAWLVSNFENLNPSNTYWTKQYNLYSKIDTEGP
                     RYLEFEQWWGGHVLLNAEEIQSIVDELFVGNKLATAEITTKDGATLDFRNIRAPIVVF
                     CSKADNITPPPQALNWILDLYRDVDDIRAHGQTIIYAVHESAGHLGIFVSGSVAKKEH
                     DEFASNIDLIEILPPGLYEAVMTPKQSDDETGELIGGDYLVRFEARTFEDIRALGTNS
                     EEDDRRFATVARLSEINLGLYRSFVQPWVRLWANEGAARWMRKLHPLRLQYELFSTAN
                     PFMSYVASMADVVRKNRQPVSKDNV"
BASE COUNT          986 a         1255 c         1260 g          980 t
ORIGIN      
        1 agcgttgagc aagttcgact accgcttctg tgctgtcggt cctgatgcgc accatgagaa
       61 ttgaccgtct ctgaccatca tggatcgcct cggcaggctg cacagttgca gctggaaagc
      121 tgcgcccgat gagttcgcaa agacgatcta cgtcgcttgc gccaagagtg acgttttccg
      181 tgtttagcaa gtagaccaga tgtaccgcct ggccgatcgc gctgtcgaga tgacgtgccg
      241 ccacctcgcg cccttcgata gagaatgagg tctcttcgcg gatagtacgc tctacatctg
      301 ccgccgtctg gtcaaagatt cgttcaagct ctgcatcagt gccgcgagcg gcgtcaacca
      361 agtgcctaca gccctcgaga taaccgagct tgcgctctgc gggccacccc gcaggaggag
      421 atactgcaat tgcacgaagg ttggagatca catcggccat cttcacaagg cgcgcttctc
      481 gtgactttag gggaatcgcc gcaatgcgcg ctcgtcgccg ctccgtcttg ggcaacgaca
      541 tgtcatcgga attttccctg acgattttgg cgacacgatc cccgaatgtt gcggcgacgt
      601 cttcataact tgtagacgta tcttcaacca catcgtgcag aagcgctgct aaagccacgt
      661 ccatgtcaac cccatcgctc gcctgcacga cgagatcaag aacctcaaca aggtggttga
      721 gatagggctc ctgtgctgca cctttccgcc gctgattacg gtgggcctca gcagcgaaaa
      781 tcaatgcctt cgtcaagcga cgcaactcag cgttcaagag ctacctcaag ccaactgctg
      841 cgcagaggtc tttcaaatgg tcatctgtgg aacgtgccca gacacaacta gtgcggcctc
      901 agtagccgtc acgacgtctt gcaccgtgat cccaggcgct gtttcgagca aggttgctcg
      961 gcgttcgggg aacgcgatta ctgcgagatc ggttacaacc agatcgactt gtcgtagcga
     1021 agttagtgga agcgtgcatt gcttaacgat tttcggcttg ccctttgccg tatgctgcat
     1081 ggcaacaacg acgcgttttg cacccgttac aagatccatc gcaccgccca ttcctggaac
     1141 catcttgccg gggatcatcc aattcgcgag acgcccttgc tcgtctactt gcaacccacc
     1201 cagcacagtc atgtcgacat gacctccgcg aattagaccg aaggacatgg cgctgtcgaa
     1261 ggtgcttgcg cctggaagcg cacttactgg cttacccgcg gcatcggtga gggtaggatg
     1321 ggtcatgcct tcctctggga tgggaccggt accgatgagc ccattctccg actggaagaa
     1381 tacgctaacg ccttctggaa cgtggttcgc cacgagggtc ggaatgccga taccaaggtt
     1441 gacgagcatc cccggccgaa gttcctgggc aatgcgcttt gcaatgatgc cttgtgcgtc
     1501 cattagctca ctcgttggcg accaggtagt caacgattgg cgcaggggtc accacgtgat
     1561 cgggcgcgat gactccgact gggacaatat gctcggccgt aacgatgacg gtatccgctg
     1621 ccgtggccat cacgggattg aagttgcgag ctgtcagagc ataggacaga ttgccgagat
     1681 aatcggcgag gaacgaatgg atcagtgcga aatcagcgcg taacggagtt tcgagaagaa
     1741 agttctttcc atctacctcg atatttcgct tgccttcctc gaccacggtg ccaacgccag
     1801 tcggcgtaag aatcccaccg agaccaaaac cgccagcacg gatgcgctcg atcagcgtac
     1861 cctgtggtac caaatcgatg ttcatcttgc ccgtgatcat ttgctgttgc gtctcggggt
     1921 tgaggccaat gtgactcgct atgaccctgg cgataagtcc agcgctcacc agtttaccga
     1981 ttcccctgcc tggtgccgca gtatcgttgg caatgacagt gaggtttcgc ttgccctgac
     2041 gcacgatttc atcgaccaga cgctcgggcg tccccacggc catgaatccg ccaatcatta
     2101 gactggctcc gtcaggaatc atggctaccg cgtcttcgag ggctatcgtc ttcatggttc
     2161 aaacttactc gcttatgggt aagcccaaag cccgcccacg cttccgtctt gccttcaaaa
     2221 actccccatc acggtataag ttagcatgac tttcgggccc atgcccctga ggattgagga
     2281 ggaattatgg tagcaagtgc ggaaaaggtg tcgggtctcc cctcagccaa agagatgatg
     2341 gaaaaactcg cccttgccga ggctaaaaag gcggaggaag cggcacgtaa aaaagccatt
     2401 gtcgagggcg aaaagaaggc gctgctcgat aacctgacca aagcgtcagg agtttccgac
     2461 gatgaggctt tgcagcgtgt gaaaactatc gttgaacgtg ctgtggcgaa cgggctcacc
     2521 gaggtactcg tctatcgttt tcccaaccag ctttgtaccg atcgtggtcg cgccatcaac
     2581 caacaagaag ccggttggga aactactttg acggggctgc ctaaagaaat gtatgagttt
     2641 tggaagcgcc agcttcagcc actcggatat aagataaggt tccagatcgt cgatttccca
     2701 ggtggcatgc cgggagatgt gggcatcacg cttaggtggg cgtgagcgga ctggacaagc
     2761 ggggaaattt tgctgccgct gcacttcagg gcagcgggaa ggggcctgcg ctcgatagtg
     2821 cgagcggagg tcttgatatt agtcacggac cccgcgctag cggagtttcg aggaggaaat
     2881 catgaatacg caaccgcaca aggcggaatt tgagccgact tccttgctct ggccagtcga
     2941 tcttgcgcga gaggcgtggg catacggcgt cgataccttg cagcgtagca tcctttttct
     3001 cgacgtactc cgcgagaggg gcaagggata tgaggagcat gctgctaaac tggcccctca
     3061 tgtcctgaaa ttcgagtgcg aactagtgat ggatgggcgc aaattaccgc gacccgtgaa
     3121 ctaccttctc gtccgcatca cacccccagc tggcgtcgaa atcgatgagc gtaagaggcc
     3181 tttcgttgtt gtcgatcctc gcgccgggca cggtcccggt atcggtggat tcaaagcgca
     3241 gagcgaaatc ggagttgctt ttcaggctgg tcatccttgc tatttcatcg gtttcctgcc
     3301 cgaaccggag ccgggccaga cgatcagcga catcgcatat gctgaagctg cgttcttgga
     3361 gcgtgtgatt gcactccacc ccaaaaccga cggcaagccg gcagttatcg gcaattgcca
     3421 agcaggctgg gcagtcatga tagtggctgc cttaaggcct gagttgttcg gcccgatcat
     3481 tgtcgcaggc tcgcccctct cctattgggc tggcatgcgg ggccagtatc caatgcgtta
     3541 cacggccggc atgctgggcg ggagttggct cacgtctctg atgggcgacc tcggcaatgg
     3601 gaaattcgac ggggcttggc ttgtcagcaa cttcgagaat ctcaacccgt ccaataccta
     3661 ctggacgaag cagtacaatc tttactccaa gatcgatacc gaggggccgc gctatcttga
     3721 gttcgagcaa tggtggggcg gacatgtcct gctcaacgcc gaggagattc aatcgatcgt
     3781 cgacgagctg ttcgtcggca acaagcttgc cacggcagag attacgacaa aggatggcgc
     3841 tacgctcgat ttccgcaaca tacgcgcgcc gatagttgtg ttctgctcca aggctgacaa
     3901 catcacaccg cccccgcaag cactgaactg gatactcgat ctctatcgcg acgtcgacga
     3961 tatccgagcg cacggacaga cgatcatcta tgcagtgcac gaatcggccg ggcatctcgg
     4021 catttttgtt tcgggctcag tagcaaaaaa ggagcacgac gagttcgcga gcaacatcga
     4081 tttgatcgaa atcctgccgc caggactcta tgaagcggtg atgacgccga agcaatccga
     4141 cgacgagact ggggaactga tcggcggcga ttatctcgtg cgcttcgagg cacgaacgtt
     4201 cgaagacatc cgtgcgcttg gcaccaatag cgaagaagat gatcggcggt ttgccaccgt
     4261 tgctcgcttg tcggagatca atctcgggct atatcgaagt tttgttcagc cctgggttcg
     4321 gctctgggcg aatgaaggtg cggcaaggtg gatgcgcaag ctacatcccc tacgcctgca
     4381 atatgaactt ttctccactg ccaatccctt catgagttat gtcgcctcca tggccgatgt
     4441 tgtgcgcaaa aaccgccagc cggtttccaa ggacaatgtc t
//
LOCUS       DASTQY010000411         6114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_694268, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000411 DASTQY010000000
VERSION     DASTQY010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694268"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFR82_13295"
     CDS             <1..271
                     /locus_tag="VFR82_13295"
                     /inference="COORDINATES: protein motif:HMM:NF025388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HEU4502622.1"
                     /translation="GLGFEPASILLPISPKRCLLLTNRPAADLKLPGCLLLPFRRFMK
                     NNGTVFKVDEVKVEAFNRLIISQAHEAVFSHERSDELERAFNQTA"
     gene            complement(337..936)
                     /locus_tag="VFR82_13300"
     CDS             complement(337..936)
                     /locus_tag="VFR82_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502623.1"
                     /translation="MRRQLPVLDLEALRRRSARQSRPQFHSLSDVIDDYISRHRTSAA
                     AEMDFFRNESFPVAIEYAALSKLPDGHRHPHEYRRKLQALEEAYRRLQAIPGEMRKCS
                     SFEELHGLIERKIGGIPDIGPLTVYVVATRIGAHLHLEPEKVYLHSGTKKGAGALGLG
                     ARRKTLEPGELPVEFGRLTAREIEDCLCLYKDYLGDFRA"
     gene            1064..1672
                     /locus_tag="VFR82_13305"
     CDS             1064..1672
                     /locus_tag="VFR82_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502624.1"
                     /translation="MNAITRFLVSAALATCVITSGCRSKSQPQEPATGNANASAPSKI
                     QSTLSIEAGLVYKSGDAKPVPRTEFYLLDADAEKILTDAKIEKKGRLKNWKEMALVKA
                     YAAAKLCVHRLTRCPALKDSWDLVRDADTALKPHIIKSVSTGFDGKASFEAAPAGTYY
                     VMGVYLLDESFVAWNVEVNLKSESQQVILDQNNALMIFQPSS"
     gene            2573..2965
                     /locus_tag="VFR82_13310"
     CDS             2573..2965
                     /locus_tag="VFR82_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502625.1"
                     /translation="MAGALDVCTYCNTPLFAKALDKSTYETLEARESQIERPDTSAWS
                     LLERHGVKNISPERAKVIVDYIRTGNTISAIKEVRGANPTYDLAGAMNVVKAVGRELG
                     VEKSGGGCVVLLLFIPCLIILSYLVDKV"
     gene            complement(3110..3283)
                     /locus_tag="VFR82_13315"
     CDS             complement(3110..3283)
                     /locus_tag="VFR82_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502626.1"
                     /translation="MAGQVGEEQIIAGGGAHHLKPFVLPGGVGGVAFLQKSGGELPSL
                     FGREFPRLLQHTF"
     gene            complement(3392..5446)
                     /locus_tag="VFR82_13320"
     CDS             complement(3392..5446)
                     /locus_tag="VFR82_13320"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU4502627.1"
                     /translation="MSSISDESCEPSKKIRVEIVTSNDPNEKVGSHGVGTERWLADEE
                     PLRYSIFFENKPTASAPAQDVVVTDQLDHASLDLTTLSLGPISFADKVVAPPAGLPLS
                     VTGQFNADVDLRPANNLIVRINATLNQNTNVLTWRFTSIDPATGQPPEDPLAGFLPPG
                     AGGSVFFTVMPKKGLPTGTNIRNKATIVFDVNPPLDTPEWSNALDGTKPTSRVLPLAT
                     IQNSCTFNVRWEGTDVGSGVRDFTVYVSDNGGPLTPWLTQTTATQAAFTGADGHTYSF
                     FSVARDLTANSEDGKTVAEATTRVVSNADISLALTAPPGSIGIGSNITYTLAVSNSGT
                     DAAAPITVTDNLPGDLTFVSCEATGGGVCGGSGNNRTVTYQSLGNGATATITLVATVS
                     SVPDGNTIINVASVSSPTVDPNPANNSATLTTVVQSFGITGRVQDTNGHALSGVALTL
                     GGSRTGAATSDAAGNYSFPGLPAGGNYTVTPAKPRYIFSPTSRNFTGLDSNQTAHFIA
                     TVVPGVPVLISEESSTRAIALGSPVWLRDPFQLNSPVPWGVDQRTRVMLFAMNFDLIP
                     GESISVVTVDAEDATHRIYPLTVEYVGNVPGFEWLRCVIVRLNDEMGNVGDVLVRVNV
                     RGVPSNRVRLGVGHTGGGPPDDPGSVPTPGRGFARRDGEDVDDREDVVEGSLTDDEYR
                     AR"
BASE COUNT         1352 a         1674 c         1727 g         1361 t
ORIGIN      
        1 agggctagga tttgaaccag cttcgatttt attgccgatc tctccgaagc gttgtttatt
       61 gctgacgaat cgtccagccg ccgacttgaa gcttccgggc tgtttattac ttccgtttcg
      121 acgctttatg aagaataacg gcactgtctt caaggttgat gaagtaaagg ttgaggcctt
      181 taatagactc atcatttctc aagcccatga agcagtgttt tcacatgagc gctcggatga
      241 attagaacgt gcttttaatc agactgctta gcccatgaga agggcgaact gccgctttgg
      301 tttatttagt taaccctgtg taaggttaag gaagcgtcag gctcgaaagt ctcccaaata
      361 gtctttatag agacacaaac aatcctcgat ctcgcgggcg gtgagccggc cgaactctac
      421 cggcagctcg cccggctcta acgtctttcg gcgcgcgccg agtccgagtg ctccggcccc
      481 tttcttcgtg cccgaatgca gatagacctt ttcaggctca agatgcaaat gcgctccgat
      541 gcgggtcgcc acgacgtata cggtgagcgg gccgatgtcg gggatgccgc caatcttccg
      601 ctcgatgagg ccgtgcagtt cttcaaacga tgaacacttt cgcatctctc cggggatggc
      661 ttgaagtcgg cggtatgctt cttcgagggc ttgcagtttc cgacgatatt cgtgtggatg
      721 cctgtgacca tccggcagtt tcgagagcgc ggcatactca atggcgacgg ggaaactttc
      781 attgcgaaag aaatccattt cggcagcggc ggacgtgcgg tggcggctga tgtaatcgtc
      841 aatcacatcc gagagcgaat gaaattgggg acgcgattgg cgagcagagc gtcggcgaag
      901 ggcctcaagg tctaagacag gtagttgtct acgcatagca acatggcatt ttattattta
      961 ctcgttccat tcgccattct ttaacttcct cgaaatagta gcgtgccgat gacaaattat
     1021 ggtttagctt ggtctcaagt tatgggttaa cttacacgaa cagatgaacg ctataactag
     1081 atttctagtc tcagccgcct tagctacctg cgtcattacc tccggctgcc ggtcgaagtc
     1141 tcaaccgcag gagccggcga ctggtaatgc caacgcatcc gcaccgagta agatacagag
     1201 caccctctca atcgaggccg ggcttgttta caaatcggga gacgctaagc ccgtccctcg
     1261 gactgagttt tatctgctgg acgctgacgc cgaaaagatt ctcacggacg ccaagatcga
     1321 gaagaaaggc aggctgaaaa attggaaaga gatggctctc gtaaaagcgt atgctgcggc
     1381 aaagctgtgc gtccacagat taacccgatg ccccgcactg aaagactcgt gggatttggt
     1441 gcgggatgct gacaccgcct tgaagccgca cataatcaaa agtgtgtcca cggggtttga
     1501 tgggaaggcg agttttgagg cggcgccggc gggaacttat tacgttatgg gagtctacct
     1561 gcttgatgaa tcattcgtcg cttggaacgt ggaagtgaac ctgaaatcag agtcacagca
     1621 agtgattctc gaccagaata atgctttgat gatttttcag ccttcatcat agtgtccgct
     1681 cacatcaggt ccctctatag tcatttagat tgctggttga atcgaaagaa gatttcctca
     1741 tgaacgagct aataccctgt cccacctgcg gcaagcctct cccgccggac tctatcctat
     1801 gtcctaaatg tcagaccaga ttgaaaaggg acaaaaccgg aaagcatttt atcgggcggc
     1861 agccatccac acccaacaat cctagaagcg aaacagttcc tcaaagagcc attccaacga
     1921 agccgagtcg gcccagagtt gggctaatca tagtcggtat tattcttgga gtggtatttg
     1981 tggcgatgta caaaagttgt gagagttcga ggcgtgattt gtccagccaa acaccctctg
     2041 gggcgtccac gtcctcaaat gtatctttgg atgtttcgtc aatcactctc aattatcaaa
     2101 cacggcaaat ggaaattcgt ggaacgcttc gtaataatgg aagctcgaag ccggataggg
     2161 tatgggtttg ggcatatttc tttgctccgg cttccgagta tgctgatggc agttggtcgg
     2221 attcgcctat cgagattggt aatccttttg cgtcgggcga tacagctaca atcacagcga
     2281 ccggccattt ccattggtgg gataatccac acacgcctaa gagtggctat tatgctcgcg
     2341 tgagtgtttc agcacagtcg ggtgaagcgg cgaaagtacc ttctgcccaa cggaatagaa
     2401 gtgccgccgg cgctcattct gttcgcgtca ttcaataatc aaggagtcgg tcatggcttg
     2461 gtgccctctt tgttcccaag accggcctat tcagcgccaa atattcgaga acacctgttc
     2521 tttctgtagg caggtgcaga cctcgccgca ccacccggat tgccgcggcc ccgtggccgg
     2581 cgctttggat gtttgcacct actgcaacac acctcttttt gctaaagcac tcgacaaatc
     2641 aacgtatgag acgctggagg cacgggagag ccagatcgag cgccctgaca cctctgcctg
     2701 gtcgctgttg gagaggcacg gcgtaaaaaa cattagccct gagcgcgcaa aagtcatcgt
     2761 ggattacatc aggacgggga acaccataag cgcgatcaag gaagtgcgcg gggccaaccc
     2821 tacttacgac ttggcgggcg caatgaatgt agtgaaagcc gttggtcgag agctaggcgt
     2881 tgaaaaatcc ggaggcggct gcgtagtcct gctgcttttt atcccttgcc tcattatctt
     2941 gagctacctc gtggataagg tctgaggggc ttgaacggcg gcccgacagc gaatagcctt
     3001 gagcgccaat aatcattttg ccgcgtgtgt tggaggccca ttatcgggtg tatcatactg
     3061 ccggtcagag gagagaaaat cagagtagag gtgacggcta atgagtaggt tagaaagtgt
     3121 gctggaggag gcgcggaaac tcgcgcccga agagcgacgg cagctcgccg cccgacttct
     3181 ggaggaaggc gacacctccc acgccgcccg gcaggacgaa aggcttgagg tgatgcgcgc
     3241 cgccgccggc gatgatctgt tcctcgccga cctgaccggc cacgatggaa gatttccagc
     3301 acgccgacgg cgacgggctg cccgcatgag catcgagcgt tggagcgtgt ggcgcgcgaa
     3361 cctcgatccg gtcgtcggct ccgagcaggg gctaacgcgc ccggtactcg tcatcagtca
     3421 gactgccctc aacgacatcc tcccggtcgt caacgtcctc cccatcacgt cgcgcaaagc
     3481 ctcgcccggg tgtgggtacc gacccgggat catctggggg gccacccccc gtatggccta
     3541 cgcctaaccg cacacggtta ctggggactc cgcgcacgtt aacgcgaacc agcacgtcac
     3601 cgacattccc catctcgtcg ttcagacgca ctatcacaca acgcagccac tcaaaacctg
     3661 gaacattccc gacgtactcg acggtcaagg ggtaaatccg gtgagtggca tcctccgcat
     3721 cgacagtcac tacggaaatg ctctccccgg gtataaggtc gaaattcatg gcaaagagca
     3781 ttacacgcgt gcgctgatca accccccatg gaaccggcga gttaagctgg aagggatccc
     3841 gcagccacac gggggagcct agcgcgatgg cgcgggttga ggactcttca cttattagaa
     3901 cggggacgcc tgggaccacg gtggcgatga aatgagcagt ttgattactg tctaagccag
     3961 taaagttgcg gctcgttggg ctaaagatat atctgggttt cgccggcgtc accgtgtagt
     4021 tgccgccggc gggcaggccc gggaaagaat aattgccggc ggcgtcgctc gtggcggcgc
     4081 cggttctcga cccaccgagc gtgagcgcca cgccgctgag ggcgtgtcca tttgtatcct
     4141 gcacgcgacc ggttataccg aagctctgaa ccacggtggt cagtgtggcc gaattgttgg
     4201 caggattagg atcaacagtc ggagagctga ccgaggcaac gttaatgatc gtgttgccgt
     4261 cgggtactga ggacactgtc gcgacgaggg taatggttgc cgtcgcgccg ttcccgagcg
     4321 attggtaggt cacggtgcgg ttgttgccag aacctccaca cacgccccca cccgtcgcct
     4381 cacacgagac gaaagtcagg tcgccgggca gattgtccgt caccgtgatg ggtgcggcgg
     4441 cgtcagtccc gctgttgctg accgcgagcg tgtaggtgat gtttgagccg atacctatcg
     4501 agccaggcgg agcggttaag gctagtgaaa tgtccgcgtt cgacaccact ctcgtcgtgg
     4561 cctccgcgac cgtcttgccg tcctccgagt tggccgtgag gtctcgggcc acgctgaaga
     4621 aagagtaggt gtggccgtcc gccccggtga aggcggcttg tgtggcagta gtttgggtga
     4681 gccacggcgt aagcggccca ccgttgtcag agacatagac ggtaaagtcg cggacaccgg
     4741 agccgacatc cgttccctcc caccggacgt tgaaggtgca ggagttctga atcgttgcga
     4801 gcgggagcac gcggctggtc ggcttcgtgc catcgagcgc gttggaccac tccggcgtgt
     4861 ccagcggagg gttcacgtcg aaaacgattg tcgctttgtt tctgatgttg gtgccggtcg
     4921 gtagcccctt cttaggcatc acagtgaaga agacgctacc cccggccccc ggcggcagga
     4981 atccggctaa ggggtcttcg gggggctgtc ccgtggcagg gtcaatggag gtgaaccgcc
     5041 aggtcagaac gttcgtgttc tggttcagag tcgcgttgat gcggacgatg aggttattcg
     5101 cggggcgcaa atccacgtcg gcattaaact gccccgttac tgataacggg agaccggctg
     5161 gcggggccac aaccttatcg gcaaatgaaa tcggccccaa gctgagggtc gtcaggtcta
     5221 agctcgcatg gtcgagctgg tccgtaacga ccacgtcttg ggcaggtgca gaggccgtcg
     5281 gcttgttctc gaagaagatg gagtaccgca gtggctcctc gtcagcgagc cagcgttccg
     5341 tacccacacc atgtgaaccg accttctcgt tggggtcgtt agacgtaaca atttcaaccc
     5401 tgatcttctt actaggctcg cagctttcgt cgctgatgct actcatcgta gcgctggtgg
     5461 catttaagcc gtcactatcg cagtcagggc aaggcactat gacccagccg ccactacagc
     5521 cggcccctgg ttgaccgtcc acacactcaa accacttcca gcaaccatct tcgagaagcg
     5581 gaggaggagt cccaccttcc tttggcgggt cagggcaatg ccacacccat ccgccgaggc
     5641 ccacccccgc aatcggattc caagtccagc aggggtcgtt atcaggaggg gctggccttc
     5701 tgacgaatcg ggcagtgatt gtcttattct cttctacttt tacctccgct tcgtttaaca
     5761 aaaatccggg gaccatatcc ccttcccagc cgacgaatct atcgtccaga ttcgctgtag
     5821 ctcgtaactt cactataagg cccttagtaa aacgagccga gcaattttcc ccacattgga
     5881 tacctcgtgg atcggacacc acaagtccgc tgccgtttcc tgtcttctga acatcaagga
     5941 acttggtact atcgaaaggg aaccgaacat gccaatgttc gcccgcgcga cctttgcatt
     6001 caaaaatgta ttctgcacct gatggactga gccactttgg atccgctaag ccatccccac
     6061 aaaaactgac atcccctgtt cgtaggtaat taggatttcc cattatgtaa ctag
//
LOCUS       DASTQY010000412         8399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_117846, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000412 DASTQY010000000
VERSION     DASTQY010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117846"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /gene="lnt"
                     /locus_tag="VFR82_13325"
     CDS             complement(<1..917)
                     /gene="lnt"
                     /locus_tag="VFR82_13325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEU4502628.1"
                     /translation="MSQWPVRLILLAATTGILLPYCFPKYDLGLLAWVALIPLHIALA
                     SSSRRSQAFWLGWLAGIVSSTGIMSWVVTAMHTYGKVPLVISYIIMLLLTAYLGLFVA
                     LYSAGVVWFRMLVPRYGLFAAPCLWVALELLRTYLFTGLPWSLLGYSQYRQLDVIQIA
                     DHLGVYGVSFLIVLTNVALAELYLWLMPLFRGFRPARLPWELVTTSAMLVGLSWAYST
                     SLIVSEGMEQLKTSLRVGVVQPNIDQAVKWDQAYRDETLKRYDRLTESFGYGVDLVVW
                     PEAATPFIYEREPVYQLQLVSMASRASAPL"
     gene            complement(1044..1385)
                     /locus_tag="VFR82_13330"
     CDS             complement(1044..1385)
                     /locus_tag="VFR82_13330"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4502629.1"
                     /translation="MTAHWNKRRRVRTPVSCAVYYSDGQLHASGMMQNLTEHGGCVSG
                     THLVTVGMELMVLLIRPAKRALMIKKATVRWVRQSHFGVELDEADCGMIGEFGDTDMK
                     PPPPPLSIQTH"
     gene            complement(1382..1810)
                     /locus_tag="VFR82_13335"
     CDS             complement(1382..1810)
                     /locus_tag="VFR82_13335"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU4502630.1"
                     /translation="MGLGTSLLYMVLLGFGSLITPGVGLSGRLLELFPRPLSEFSHVP
                     AYGLLTWLLASGLRGRQWPRSIALCVAVQAALVFGIWMEVFQGFVPGRVVEIGDVLMN
                     AMGIGAASLLVGLRLLPIDEHSRRWGIRRLMESKRGCSTS"
     gene            2156..2818
                     /locus_tag="VFR82_13340"
     CDS             2156..2818
                     /locus_tag="VFR82_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502631.1"
                     /translation="MRILVVFFTLFALCTVNGCVSQSTYDAVVQEGLMVRSELDRIQE
                     EHKALVRQANDLERMNADSMREAETIVAAVQQAKDDAEAEHHAAEIRVARLKAKAAQA
                     AKQHNALQYELNVAKENTGALQEMIDVYQRKMRDGASTHPAQATEPAVHKPFDPSTIP
                     PLQDLPAPSAVEPPKPAAPPPPASAPAASPTQRTQDAVDIGWFDSIKGWLVSLWRSVF
                     SS"
     gene            3666..5717
                     /locus_tag="VFR82_13345"
     CDS             3666..5717
                     /locus_tag="VFR82_13345"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HEU4502632.1"
                     /translation="MSKNHTAAQLSRSATLDPAVSDKALPKKPPQQVTAVEMGARQRE
                     ISVSEFFTKNRHLLGFDNPRKALLTCVKEAVDNALDACEEAGILPEVSVELEVVSNGE
                     PVAPSQASKFRITVTDNGPGIIRQHIPRIFAKLLYGSKFHRLRMSRGQQGIGISAAGM
                     YGQLTTGKPVKIISRTGPRAAAHFFEVQIDTKKNEPLVHENKQIQWDQFQGTQVTLEV
                     EGKYQKGRASVDEWLEQTSIANPHVRLVYHTPEGETKEYSRSYHELPPSPREIKPHPY
                     GIEFGMLLKMLQDTKSHSLSNFLASDFCRVSPQLAVDICKEAKVSPHLKPRDLKGPAA
                     ETLYRTVQSTKLMAPPTNCLSPIGEKAILSGLYKQIKGEFYTAVSRPPAVYRGNPFII
                     EAGLAYGNRPGDQPKPEQAAGPKAEGEDEDDNTELARVIRYANRVPLLYQQSACSTFK
                     AVLTTTWKNYGVPQSRGALPAGPMVIFVHMASVWVPFTSESKEAIADYDEIQKEITLA
                     LRECGRRLGLFLRRRERAASEYRRRNIFELYIEEVVEACNRLKGGKLPKDKIKMQLQK
                     IANSRTGGLKTDEALGKTGAGPEGLPHSIIVTEAGIEGEVQAAPENVEPATTGPALEG
                     DLLGGSKPDALPAKRKSGAARQELKKGTKKDVRGKSEQIGLFADKSTPGVRPAARRRR
                     K"
     gene            5723..6826
                     /locus_tag="VFR82_13350"
     CDS             5723..6826
                     /locus_tag="VFR82_13350"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU4502633.1"
                     /translation="MTTKQRRPGPVEKKLIGLADIVISAADRSRDPTFAIPIRALSNV
                     SFNERKGLIEMGHKKQARSFFNVGMAKKFMQTVLVADALSELQRADLTTSLREIYYRT
                     KHTIQDSHENTFDTQDESDPVIEDLEVSLAALREELHVRAENSGSIVGPVVFGDDGDR
                     VDCARLGKGGYSVPSIVEPEYLEIRRCTADFLLLVEKGTQWNRLSEDKFWRRYNCVLL
                     TGNGQPPRGVRRLARRLHEEHRLPVYVLVDNDPWGYYIYSVVKQGSINLAFESQRMAI
                     PKAKFIGLSSADPERYQLPRNVGIKLNEKDIARAKELMNYQWFQKPAWQIEIKRMLTS
                     GLKYELDALANKDFQYLTKKYLPRKLKEKDWLD"
     gene            7360..7593
                     /locus_tag="VFR82_13355"
     CDS             7360..7593
                     /locus_tag="VFR82_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502634.1"
                     /translation="MTIAGCRVRLAYCRASDERWIVKGTMQCGLEDQQGERSIVTEAY
                     PSREEAEQYAIERVTTLLGQHTDRSHSRVRNWS"
     gene            7629..8195
                     /locus_tag="VFR82_13360"
     CDS             7629..8195
                     /locus_tag="VFR82_13360"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU4502635.1"
                     /translation="MAIIGQAIPDGTYQAFHDNEIRSITLDSFRGRWLVLVFYPGDFT
                     FICPTELEELGALYGDFKKLDAEVIGVSTDSVYVHKAWHDTSPAIKKLPFPLLADAGG
                     SLSNKLGVYLYREGVALRGSFIFDPDGKLCAYEVHANNIGRSMEELLRKLQAASFVHQ
                     NGGAEVCPAGWKPGDRTLRPSLDLVGKL"
BASE COUNT         2082 a         2403 c         2292 g         1622 t
ORIGIN      
        1 agcggggctg aagcacggct ggccatggac acgagttgca gctgatacac cggctcgcgt
       61 tcgtagatga agggggtcgc cgcctccggc cataccacga gatcgacgcc gtagccgaac
      121 gattctgtca gtcggtcata acgtttaagc gtctcatccc ggtaggcttg atcccatttc
      181 accgcctggt caatgttcgg ttgcaccacg ccgactcgca atgaggtctt gagctgctcc
      241 atcccctcgc tgacgatcaa tgaggtacta taggcccagg acaggcccac cagcattgcc
      301 gaggtcgtca ctaattccca tggaagacgc gccggcctga atccgcgaaa gaggggcatc
      361 agccacagat ataattccgc cagggcgaca ttggtcagca caatcagaaa cgacacccca
      421 tagactccca aatgatcggc gatctgaatc acgtcgagct gacgatactg tgaatacccg
      481 agaagcgacc agggcaaccc tgtgaagaga taggtgcgca gcaattccag cgccacccac
      541 aaacagggtg cggcgaacag gccgtatcgc ggcaccagca tccggaacca tacgaccccg
      601 gcactataca gcgcgacgaa aagacccaga tacgccgtca acaacagcat aatgatgtag
      661 ctaatcacca aggggacttt gccgtaggta tgcatagcgg tgacgaccca ggacatgatg
      721 ccggtggaac tgacgatgcc ggcgagccac cctaaccaaa acgcctgcga gcgtcgactg
      781 ctgctagcca aggcaatatg aaggggaatc agggcgaccc aggccagaag gccgagatca
      841 tatttgggaa agcagtaggg aagcagaatg cctgtcgtgg cggcgagcag gataaggcgg
      901 acgggccact gactcatcgc gctaacttag cggaacgaca aaggccgttg caaccaacgc
      961 ttgcgcagaa agtgacgatt gagcggctac tctgctcgac gagaagcgcg gtgccgtgcg
     1021 ctcacccgtc cgcagaaagg gtgctaatgg gtctggatcg agaggggagg cggtggtggc
     1081 ttcatatccg tatcgccgaa ctcgccgatc attccgcaat ccgcttcgtc aagttctaca
     1141 ccgaaatgtg actgacgcac ccagcgcacc gttgctttct tgatcatcaa cgcacgcttg
     1201 gcaggacgaa tgaggagcac catgagctcc atacccacgg tgactagatg ggtgcccgat
     1261 acacaaccgc catgctctgt gaggttttgc atcataccgc ttgcatgcaa ctgcccatca
     1321 gaatagtaga cggcgcatga cacaggagtg cggacgcggc gtcgtttgtt ccaatgagca
     1381 gtcacgatgt gctgcatcct ctcttggatt ccatgagtcg acgaatcccc cagcgacggc
     1441 tgtgttcgtc gatgggcagc agccgcagcc cgaccagaag cgatgccgct ccaatcccca
     1501 tcgcattcat cagaacatcg ccgatttcca cgactcgacc cggcacgaag ccttggaaca
     1561 cttccatcca tatcccgaac accagcgccg cctgtacagc cacgcacagg gcgatacttc
     1621 gaggccactg tcgacctcgt aagccactag cgagcaacca cgtcaacagc ccataggccg
     1681 gcacatgcga aaattccgag aggggacgag ggaacaattc caatagacgc ccggaaagtc
     1741 ctactcccgg cgtaatcaga gagccgaatc ccaagagcac catatagagc agtgacgtac
     1801 ccaagcccat gcatgactcc aatgagaacg tgagagaaac ctacaagatc acttactgag
     1861 aaactattgg gcaatggtag ggcctgagag agggattagg atgtgaagaa cgtctagctt
     1921 gctgggttcc ggcgacgata attggagtac attctgtgca taatcatggg gttagacctt
     1981 gcatttacct acctatattt tgactgtcgc cgggcaagcg tttctgccac ctttcgaggg
     2041 agagcacgaa cacatcaagt tggtgatttc tcacgagtga acgggtattc tttcgttcag
     2101 acagatttga gcagattcgt tggttgagag acacacaaac aactcagata aactgatgcg
     2161 catcctcgtc gtcttcttca ctctgttcgc tttgtgtacg gtcaacggct gtgtcagcca
     2221 aagcacctat gatgcggtcg tgcaggaagg cctcatggtc aggtctgaac tcgatcggat
     2281 tcaggaggag cacaaagctc tcgtccgtca agcaaatgac cttgaacgga tgaacgcgga
     2341 cagtatgcgc gaggccgaaa ccatcgtggc agccgtacag caagccaaag atgacgcaga
     2401 agccgaacac cacgctgccg aaatacgggt cgcgaggttg aaagcgaaag ccgcgcaagc
     2461 cgccaaacag cacaacgcac ttcagtatga gttgaatgtg gccaaggaaa ataccggcgc
     2521 actgcaagag atgatcgacg tatatcagcg gaaaatgcgc gatggagcat cgactcaccc
     2581 ggctcaggcg accgaacccg cggttcacaa gccgttcgat ccttcaacaa ttccaccgct
     2641 gcaagatcta ccggctcctt cggcggtgga accgccgaaa cccgctgccc cgccgcctcc
     2701 tgcgtcggcg ccggccgcaa gtcctaccca gcgcacccaa gatgccgtcg acataggctg
     2761 gtttgacagt attaagggat ggctcgtttc actctggcgc tccgtcttct cctcttaggg
     2821 cttctactct cttccatcat ttttcttttg tcttgtgctt ttcctagatg ccgttgccgg
     2881 gcagtcagct cgcgcgacga gtgcgcaatt cagtaacgtc ttggcaggct tgccgtagct
     2941 gcttcgatgg tgagataacc cttttggctg tggatatttg ttggaccgca tgctcgaaaa
     3001 agcgcttcta cagcggtgac gcagcgccat cttgcggtag gcacagcaat tgatctgccc
     3061 attctattcc acaccattag tggtggtgaa ttaccaaaca gggcatatgc ccaaaaatta
     3121 ggcacttcgc tctgcagatg cgtgtaacag gactgtcctg accctagaat gaagttgttc
     3181 acagacttat ccacagaaaa tggggatcga gaaatcggca cattgcagac agaagaagtg
     3241 catccaacag gaggagctaa gatagagaca cgaggcacgg cgtctcggtg tctgaagatt
     3301 tgattcggaa ggctgttggc aagactgtta ggagtgggag gagcacgcac ctaccgagag
     3361 tgataggtac ggtggagacc ttgggaggac tttaggcttc ccagtacaag ctgggcctgc
     3421 acaccatcct catcgctcgg tcccctaatg gaccacattc cctctgggcg ttaactcctc
     3481 ccacaagaaa tgacgatagg cttctcctca cagctcgtca agcaaactaa tataaagagc
     3541 gagcctctca agttatattg actcacacag tcttagaagc gagaacgacg accggacgaa
     3601 tcacattgcc tcgcgctatc ttttgtgata ccaagacgca ctcgccccat accatacgag
     3661 gtggaatgtc gaagaaccac acggccgctc aactttcccg atccgcaaca ctcgatcctg
     3721 ccgtttccga caaagccctt cccaaaaaac cgccgcaaca agtgacggcg gtggaaatgg
     3781 gcgcgcggca gcgggaaatc tccgtttcgg aatttttcac taagaaccgg catctcctcg
     3841 gttttgataa tccacggaag gccctcctca cgtgcgtcaa ggaggcagtg gataacgcct
     3901 tggacgcctg tgaagaggcc ggtatcttgc ccgaggtgag cgtcgagctt gaagtggtct
     3961 ccaacggaga gccggtcgca ccaagccagg ccagcaaatt ccgtatcacc gtgacggaca
     4021 atggccctgg catcatccgg cagcatattc cacggatttt tgcgaaactc ctttacggct
     4081 caaaatttca tcgccttcgt atgagccgcg gtcaacaagg catcggcatt tccgccgccg
     4141 gtatgtatgg tcagctcacg accggaaaac ctgtcaaaat catttcgcgc accggcccac
     4201 gagcagctgc tcacttcttc gaagttcaga tcgacaccaa gaaaaatgaa ccgcttgtgc
     4261 acgagaacaa acagatccaa tgggaccaat tccaaggcac gcaagtcacc ctggaagttg
     4321 agggcaaata tcagaaaggc cgggcttcag tcgacgaatg gctggaacag acgtctattg
     4381 cgaatcccca cgtccgactc gtgtaccaca cacccgaagg cgaaacgaag gaatactccc
     4441 gttcgtatca tgagctgccg ccctcgccgc gcgaaatcaa accgcatccc tacgggattg
     4501 aattcgggat gctgctcaaa atgttgcagg acaccaagag ccactcgttg tcaaactttc
     4561 tcgccagtga tttttgccga gtctcgcctc aactggccgt cgacatctgc aaggaggcga
     4621 aggtttcccc gcatctcaag cctcgtgacc tcaagggacc tgcagccgaa acactgtacc
     4681 ggacagtcca aagcacgaaa ctcatggccc cccccaccaa ttgtctttct ccgatcgggg
     4741 agaaagccat cctgtcaggc ctctacaaac aaatcaaagg agagttctat accgccgtca
     4801 gccggccgcc ggctgtctat cgcggcaatc ccttcataat cgaggctggc ctggcctatg
     4861 ggaaccgacc aggcgatcag cccaagccgg aacaagcggc gggaccgaag gccgagggtg
     4921 aggacgagga cgataacacc gagctcgcac gagtcatccg ttacgccaat cgagtgccct
     4981 tactgtacca gcaatccgcc tgttccacat tcaaagccgt cctgaccacg acctggaaaa
     5041 attatggcgt cccgcaatcg cgcggggccc tgccggccgg tccgatggtc atcttcgtcc
     5101 atatggcctc cgtctgggtg ccctttacaa gcgaatccaa agaagccatc gccgattatg
     5161 acgagataca gaaggagatc actctcgcct tacgtgagtg cgggcgtcgg ttgggattgt
     5221 tcttgcgacg gcgggagcgg gcggccagcg aataccggcg gcgcaatatc ttcgaattgt
     5281 atatcgaaga agtggtcgaa gcctgcaacc ggctcaaagg cgggaagctg ccaaaagaca
     5341 aaattaagat gcagctgcag aaaatcgcga attcacgtac cgggggcctc aaaaccgatg
     5401 aggcccttgg caagaccgga gcaggtccgg aaggccttcc acattcgatt atcgtgaccg
     5461 aagcaggcat tgaaggagag gttcaagcgg ctccggaaaa cgtagagccg gccacaactg
     5521 gcccggctct cgagggcgat ctccttggcg ggagcaagcc ggacgcactg cccgctaaac
     5581 gtaaaagcgg cgcagcgaga caggaactga agaaggggac gaagaaggac gtgcgcggta
     5641 agtccgaaca gatcgggttg tttgcagaca aatcgacccc aggcgtgcgg cctgctgcgc
     5701 gccggcgaag gaagtaactg ccatgaccac gaagcagaga cgcccgggtc ccgtcgagaa
     5761 gaaactgatc ggtctggccg acattgtgat ttcggctgcc gaccgatcga gggatccgac
     5821 gttcgccatc ccgattcgcg cgctctcgaa tgtgtccttc aacgagcgca aaggcctgat
     5881 cgagatgggc cacaagaaac aggcccgttc gttcttcaac gtcggcatgg cgaaaaaatt
     5941 tatgcagacg gtgctggtgg ctgacgcgct atccgaactg cagcgcgcag atctgaccac
     6001 gtccctgagg gaaatttact accggacgaa gcacaccatc caagattcgc acgaaaacac
     6061 tttcgacacg caggacgaat ccgacccggt catcgaagac ctggaagtct cgttggcggc
     6121 gttgcgcgaa gaactgcacg tcagggccga aaacagcggc agcatcgtcg ggcccgtcgt
     6181 cttcggcgat gatggagatc gggtcgactg cgcacgcctc ggcaagggag ggtactcggt
     6241 cccttcaatt gtcgaacccg aatacctgga aatccgacgt tgtacggctg attttttgct
     6301 gctggttgaa aagggcacgc agtggaaccg tctgtcggaa gacaagttct ggcgccggta
     6361 caattgcgtc ctgctgacag gaaacggtca accccccagg ggtgtcaggc gactggcgag
     6421 acggctgcat gaagaacatc gactgccggt ctacgtcctt gtcgataacg atccctgggg
     6481 ctattacatc tattcggtcg tcaagcaggg atcgatcaac ttggcgttcg agagtcagcg
     6541 catggccatc cccaaggcca aattcatcgg attgtccagt gctgatcctg aacgttacca
     6601 gttgccccgt aatgtcggga tcaagctgaa cgaaaaggac atcgcccgcg cgaaagaact
     6661 gatgaactac cagtggttcc agaaaccggc ctggcaaatc gaaatcaagc gcatgctaac
     6721 cagcggcctt aagtatgaac tggacgcctt agccaataag gactttcagt acctgacgaa
     6781 aaaatacctt ccgcggaagt taaaagaaaa agactggctg gactaactgc caccgtccac
     6841 actcaggcac cagcctctct gtagacttct gaccattgtc aactgagctg tggtctctca
     6901 gctccggctt accaaggcgg acgtcggttt tctattcgtg gacaggagag caggggattc
     6961 acccagtagg caagctccac gacgtgggga tagcgtgata gttatgttgt atgtatgtct
     7021 attgactctg atcctcatcg ggccgcccgc ttttgcggcg gacattgtgc cggcaccgga
     7081 ctcttcgccg ccggcggcta aacaaccacc ggccactgag gcgcctcgag cgaacacggt
     7141 ccccagtcct acggacccag gcatggtcaa gcaaccggat acggtccccc atcctgattc
     7201 agtcgttacg cctccggttg tggaccccaa gatggccatt aacccagaag cggcaaacca
     7261 acaaacggaa tcctctgctc ctcctcccca agactcgtcg ccgaagaaac catgatcgat
     7321 gagagcgata gcactcgcct cagcgatgat gtcaaggaga tgacgatcgc aggttgccgg
     7381 gtgcgcttgg cgtattgccg tgcgtcggac gagcgatgga tcgtcaaggg tacgatgcag
     7441 tgcgggcttg aagatcaaca aggcgagcgc tcgatcgtca cggaagcata tcccagccga
     7501 gaagaagcgg aacagtacgc aatcgaacgg gtcacgacgc tgttgggtca acacaccgat
     7561 cgcagccatt ctcgcgttcg caattggagc tgacagcagt tagcactaat atccagacgg
     7621 aggtcggcat ggcaataatc ggacaagcga ttcccgacgg cacctaccag gcctttcacg
     7681 acaatgaaat tcgctcgatc acactggaca gttttcgcgg ccgatggctg gtactggtgt
     7741 tctatcctgg cgatttcacg tttatctgcc ccactgaact agaagaacta ggcgcgctgt
     7801 atggggactt caaaaagttg gacgccgaag tcattggcgt cagtacggat tccgtctacg
     7861 ttcataaggc atggcatgac acatcccccg cgatcaaaaa actgccgttc ccactgctgg
     7921 cagacgcagg cggcagcttg tccaataaac tcggggtata tctctaccgg gaaggcgtcg
     7981 ccttgcgcgg cagcttcatc tttgatcctg acgggaagct ctgcgcttat gaagtccatg
     8041 ccaacaacat cggacgaagc atggaagagc tgttgcgcaa gcttcaggct gcgtcattcg
     8101 tccatcaaaa cgggggagcc gaggtgtgcc cggccggttg gaagccgggc gatcgaaccc
     8161 tgcgtccaag cctcgatctg gtcggcaaac tttaggttcc gatgactgtg acctttcaat
     8221 cttggagatg gccgcttatt gcggttttcg tggcaatcgt ggcagcagcg agtgcctgcg
     8281 aaccatcggc cacatccatc ggaaggaact cgctcaaaca gcataccgga gagccgggga
     8341 gtaccgtgcc aaagacaaac cctccgtact atcccaatac ctcaggcaac atcgtcgcg
//
LOCUS       DASTQY010000413        12543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1100076, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000413 DASTQY010000000
VERSION     DASTQY010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 12543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12543
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100076"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFR82_13365"
     CDS             <1..544
                     /locus_tag="VFR82_13365"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEU4502636.1"
                     /translation="DMASHTSPLSPTRHLQSTKPPWTILVVLKAGQRAESAIKIPDGY
                     ILRVVRGTKCRYTATLFSEFANAMDFPDYFGHNWDALEECLTDLEWLPGKGYVLLVTA
                     AEQILTDDDEEFATFLEVLSDAGEAWASGQAGSGDKRAKPFHTVLAVSERDQSKRAHW
                     GIPQVTEIPTPRSSGKRRRV"
     gene            complement(566..808)
                     /locus_tag="VFR82_13370"
     CDS             complement(566..808)
                     /locus_tag="VFR82_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502637.1"
                     /translation="MKIVCSWCRKEGKHEFVGEKAPFDDARETHGICVLHRNEIREHW
                     QASTRYTGTSTFLRAATTFTLLRWSGLLNFTKNKRA"
     gene            1089..2372
                     /gene="hemL"
                     /locus_tag="VFR82_13375"
     CDS             1089..2372
                     /gene="hemL"
                     /locus_tag="VFR82_13375"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEU4502638.1"
                     /translation="MKITRSIKLFEKAQHLIPGGVNSPVRAFRSVGGQPRFIKRAKGA
                     RLYDLDGNTYIDYVLSWGPMILGHASPTVVAAIKNAAGQGTSYGAPTELEVELAGQIR
                     DAFPSMEKSRLVSSGTEAVMSAIRVARGFTKRDGVLKFEGCYHGHSDYLLAKAGSGLT
                     TLGIPDSLGVPADFAKHTLTAPYNDIRAVQRILRDRGKDLACIIIEPVAGNMGVVPPA
                     ADFLPALRQLATDYDILLIFDEVISGFRVHYGGAQALYGVTPDLTVLGKIIGGGLPVG
                     AYGGRSDIMDLIAPAGPVYQAGTLSGNPLAVTAGLATLKQLRAKNLYKQLEERSAALA
                     RGLADEAKRAGIALTHARVGSMLTSFFTQEPVVDWNSAKKSDTKRYGLFFHGMLEQGI
                     YLAPSQFEAAFLSTAHTSADIDKTIRAARVAFKSL"
     gene            complement(2553..2783)
                     /locus_tag="VFR82_13380"
     CDS             complement(2553..2783)
                     /locus_tag="VFR82_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502639.1"
                     /translation="MEMFGRVGLVEIPILIAPDQKVWMDKMIKEGKIAIPPGGTLEKG
                     SLYSMFIRMLLHNAMEEQKRQEALEADDEDDE"
     gene            complement(3430..3822)
                     /locus_tag="VFR82_13385"
     CDS             complement(3430..3822)
                     /locus_tag="VFR82_13385"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HEU4502640.1"
                     /translation="MEIKIYYEDTDCGGVVYYANYLKYFERARTHYLEARGLSVGELM
                     KQGCLFVVVHAEVDYRAPARYGDILDIATMIPDISPASMTFSHVVRERQSGRVIVEGS
                     ARLAVTDGNGKVKRLDKEMVVALQSNHG"
     gene            complement(4047..5303)
                     /locus_tag="VFR82_13390"
     CDS             complement(4047..5303)
                     /locus_tag="VFR82_13390"
                     /inference="COORDINATES: protein motif:HMM:NF021207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02710 family CRISPR-associated CARF protein"
                     /protein_id="HEU4502641.1"
                     /translation="MAQDQPVAALVVALKDDLSAVVYSLNRLRPECVCFVLPESAKAL
                     VETDVQPHISHMPRRWDWVPLSDVDHFPSCYQTLARSLPSMLRAWEIGAGALVVDMSG
                     ATAAMAGALTLITMPWTSRIVSLVEQREGREGDPAALEAGERLWISVNPWDEGAEVAR
                     REASDLFNRGAFVAAGAVFRRIESRVSGGQKPLYRALADLAEGYDLWDRFQHRQAWEK
                     LKVATKAIEMASLFGGPPGLNAGLPAVKANAGFLEKLVLDPAEVKELLAFDLLAHAGR
                     KLRVANDAEGAMQSLVRALEAFAQRQLFKQHRIKTWDVQPEQLPHPLQDVCRSCYLDD
                     IDGKYVLPLQAQFRVLAGLGDQMGESFLRQWPTMKPLFNAANRAVLGHGGEPVKAERV
                     QQLFDVVMKVTHADETSLPRFPTLSV"
     gene            5428..5862
                     /locus_tag="VFR82_13395"
     CDS             5428..5862
                     /locus_tag="VFR82_13395"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEU4502642.1"
                     /translation="MVWWYWMVLGLALLGAEMTTPGGFYILFFGLSALVVGTLAGLEM
                     VEAAWLQWLLFSVIAVGSLLIFRGPLLSRLRDNGRGGADIDSMVGEVAIPLETLAVGA
                     TGKAELRGTTWSAKNVGTTTFSKGQRGKVTKVEGLTLWIVAE"
     gene            6020..6958
                     /locus_tag="VFR82_13400"
     CDS             6020..6958
                     /locus_tag="VFR82_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HEU4502643.1"
                     /translation="MSAGTWVVLVLALLVLYVIAKTAVVVPQQSAYVVERLGKYSDTL
                     NAGFHILLPFLDSIRYRHSLKETAVDIPEQVCITRDNVQVSVDGVLYLKVLNPERASY
                     GISDYHFALSQLAQTTLRSEIGKIELDRTFEERTNINIQVVNELDKASEPWGVKVLRY
                     EIKNITPPKDVLNAMEKQMRAEREKRALILTSEGERDAAINQAEGEKQQVIKASEAKK
                     QQQINEAEGAAAAILAIAQATAEGLRKVADTIQLPGGQEAVQLRVAEQYITKFGELAK
                     TNNTLILPASVSDVGSMIAVAMNAIRQTTPSAPGKS"
     gene            7052..7630
                     /locus_tag="VFR82_13405"
     CDS             7052..7630
                     /locus_tag="VFR82_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HEU4502644.1"
                     /translation="MKDFVIQAFTDSAAVKQQFVREYADRIVEVAKLLVRTFREGHKV
                     LLFGNGGSATDAAHIAAEFVGRYKRERAPLPAIALATDVAAITCIANDYGYDELFARQ
                     VRAHGQKGDLAIAISTSGNSLNVLKGVEAAKACGMTTVAWTGGTGGKLAALVDYPFVV
                     PSKVTARIQESHITLGHVLCELTEEELLGKAS"
     gene            7614..8606
                     /locus_tag="VFR82_13410"
     CDS             7614..8606
                     /locus_tag="VFR82_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502645.1"
                     /translation="MAKPPDRIGHSLSPRRLISPINVSDLKTYPLKKRHSKVRVSDFA
                     KPWRKGGSFSLFYRALPDILAVRTLRAVAGAIARAHRKGRPVIVGIGAHVIKVGLTPI
                     LVTWMEQGLVTAVAMNGAGIIHDFELALMGHTSEEVDDEIDDGRFGMTEETGRILNEA
                     IAQGHRNGQGMGEAIGHYINRLKSQFPNRGTSILATGATFGIPVTVHVAVGTDVIHMH
                     PAADGAAIGATSLLDFRRLAAVVAGMEGGVYLNIGSAVVLPEVFLKTVSLGRNLGHTL
                     SKITTVNMDFLSHYRPLTNVVRRPTQKGGKGYSLIGHHEIMLPLLVAAVAEELG"
     gene            8613..9275
                     /locus_tag="VFR82_13415"
     CDS             8613..9275
                     /locus_tag="VFR82_13415"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HEU4502646.1"
                     /translation="MTGHADPLSVSRITAIWCDLNNRYFGGALPPIAVVWSRRLTASV
                     GMFVSRAGPRSAWRANASPKSREIRLSLPLLTPLLAGTPYGTQELVSTLAHEMIHQWQ
                     FDVLKRRPNHGPDFLRKMTEMNRDGALAVTIYHSLKQEVLALSRFAWRCGRCGRIYRR
                     QRRTIQPRRHHCGSCRGALYELSSSEAGQPSSSETDRLRPPCTSIQPARQLELDFSSA
                     SL"
     gene            9313..10470
                     /locus_tag="VFR82_13420"
     CDS             9313..10470
                     /locus_tag="VFR82_13420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU4502647.1"
                     /translation="MLRWPRQILFGDVDAMFASAATVKDPSLAGKPIAVGGPPPRGII
                     AAASYAVRAFGVRSAMPTAEALRRCPHLQLIPPDRPLYRRLHDEMRRVTDRLFPVTEW
                     GSIDEFYADTTDLQTRHPDPRLLAQLVKDSIFKATGLRCTIAVATGKTVAKIAADASK
                     PDGLAIVEPDREAAFLLPRPLRSLPGIGPKTAARLEPFSIKTISDLLDPRWDQVLARM
                     FGARLLWIRELAQGRDREPVIGDRESKSSSHETTFERDTADPTFLEDVLRGFLRMLSR
                     DLRQEGLAAEGCTVKLKDARFAVTTRQRRFPHPLNYDPEMWPTVRQALHELIKPHAHY
                     RLAGLTLFSLVPVPAGLYDQRRRKAVQAMDAIIARHGAVIGLGGVCDDDNE"
     gene            10519..11001
                     /gene="rfaE2"
                     /locus_tag="VFR82_13425"
     CDS             10519..11001
                     /gene="rfaE2"
                     /locus_tag="VFR82_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEU4502648.1"
                     /translation="MSGKVVTSAELVPFLKRARAQKQRVVFTNGCFDLMHIGHTRYLQ
                     AAKALGDILVVGVNSDASVRSLNKGTDRPIVGEAQRAEVVAAMGAVDYVVIFPEPDPL
                     ALITTLEPDVLVKGGDWTLERIVGREVVEQRGGTVKTIPLVPGMSTTALIQRIRSQPH
                     "
     gene            11142..>12543
                     /locus_tag="VFR82_13430"
     CDS             11142..>12543
                     /locus_tag="VFR82_13430"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEU4502649.1"
                     /translation="MHPRAGTLGSEPWLIVTKTDEAAERLFEDLRFYHGLHGVPVTAL
                     ALFPRWETLPYESTAPHVGLVARRMNALHQIRSRPAAKVVTSIEALMQRLMPMDTFLR
                     TVLQFKVGAGIEREVLTAGLLRLGYRRVSVVEIPGEFSIRGGIVDIFSTAYAEPLRVE
                     FLGETVESLRLFDPATQKSTAKMDRAMVLPAREYLKPDTAADALAPISADAEWRAPDL
                     YGQMHSLLDYFTTQPILALDQPAALKQSCADLWERIDDGYLRHSDKEEVSYPSPERLF
                     LTWQNIMDRTAECTQLALEPLAEAEGSWEPVQAFPGQVPASAGLGARGTPFSQTLSIL
                     EHLREACRVLLVARSRGQVDRLLALLREHDVPAIEWSASSWSTSTETRAPFFVLNGDL
                     SAGFLSSELRLAILTEEELFAKGARHKPQAKSKAATFLSSLEDLNVGDYVVHVQHGIA
                     KYRGLKRLSVQDFDSDY"
BASE COUNT         2561 a         3779 c         3649 g         2554 t
ORIGIN      
        1 cgacatggct tcgcacacat cgccgctgtc tccgacacgc catttgcaat cgacgaaacc
       61 tccctggacc atcctggtgg ttctgaaggc cggacagcgc gccgagtccg caattaagat
      121 tcctgatgga tacattctgc gggtcgtgcg gggcaccaaa tgtcggtaca cggcgacgct
      181 cttttctgag ttcgccaatg cgatggattt tcccgactac ttcggccaca actgggatgc
      241 gcttgaagaa tgtttgaccg accttgaatg gctgccgggc aaggggtacg tgctactggt
      301 cacggcggcg gaacagattc ttaccgacga tgacgaggaa ttcgccacgt tccttgaagt
      361 gctgagcgat gccggagaag cctgggcatc ggggcaggcg ggttcggggg ataaacgagc
      421 taaacctttt cacacggtat tggccgtgtc cgagcgcgat caatccaagc gggcgcattg
      481 gggaattccg caggtaacgg aaataccgac cccgcgatcc agcgggaaac gccggagggt
      541 ataatcggat aagagaacgg ccggattacg cccgtttgtt ttttgtgaaa ttcaagagcc
      601 cggaccagcg aagcagcgtg aaggtcgtgg ccgctctgag gaaggtactc gtgccggtgt
      661 accgagttga ggcctgccag tgctcgcgga tctcatttcg atgcagcaca caaatgccgt
      721 gagtctcacg cgcatcatcg aagggggcct tctctccgac gaactcgtgc ttgccttcct
      781 tccgacacca ggaacacaca atcttcattc gaaatcctta acctgtgaat aacttctgaa
      841 accatgtacg catctcgact ctacgcaata gtcaggccgc gaggcaaaga cgcgaggaac
      901 cctacgaatt gctgtatatc cgccacttct gttgcatcaa cacccacggc aactgtatca
      961 aaatggtgat gggggacgta tctgttttta cactttttga cctcacgtga ggcaattctc
     1021 ggcaaacttt ctccgcagct ggcctcgctt gaccctctaa cagtgccatg ctacggttcg
     1081 cggcctttat gaaaatcaca cgttctatca agttgtttga gaaagcccag catttgattc
     1141 ccggaggcgt caacagtcct gtccgggcct tccgatctgt cggaggtcaa cctcggttca
     1201 tcaagcgggc caaaggcgcg agactttacg atctggatgg aaatacctac atcgattatg
     1261 tgctctcctg ggggccgatg attctcggcc acgcctcgcc gactgtcgtc gccgcgatca
     1321 agaacgctgc cggacagggc acaagttacg gagcgccgac cgaactcgaa gtcgaactgg
     1381 ctggccagat ccgtgatgct ttcccctcga tggaaaaaag tcggctcgtc agctcgggaa
     1441 cggaagcggt gatgagcgcg atcagagtcg cacgtggttt cacgaagcgc gatggcgtct
     1501 tgaaatttga aggctgctac cacggccaca gcgactacct gcttgcaaag gccggctccg
     1561 gcctgacgac gctgggcatt ccagattcgc tgggtgtccc ggccgatttt gcgaaacata
     1621 cgttgacggc tccgtacaac gacattcgcg ccgttcaacg gattctccgg gaccgtggaa
     1681 aggacctggc ctgtatcatt atagaacccg tagccggaaa tatgggcgtc gtccctcccg
     1741 cagctgattt tctgccggcg cttcgccaat tggcgaccga ctacgacatc ctgctgattt
     1801 tcgacgaagt aatttccggc tttcgagtgc actatggcgg ggcgcaagca ctctatggcg
     1861 tcacacccga cttgaccgtg ctcgggaaaa tcatcggcgg cgggctgccg gtgggagcct
     1921 atggcggacg gagtgacatt atggatctca tagcgccggc tggtccggtc taccaagcgg
     1981 gaacgttatc ggggaatcct ctggcggtga cagcaggact tgcgacgttg aagcaattgc
     2041 gggcgaagaa tctgtacaag cagcttgagg aacgctcggc cgccttggcg cgaggcctag
     2101 cggatgaagc caaacgggcc ggtattgcgt taacccatgc gagagtcgga tcgatgctga
     2161 catcgttttt tacgcaggag ccagtcgttg actggaacag cgccaagaaa tccgacacga
     2221 aacggtatgg actgtttttt catgggatgc tcgagcaggg catctatttg gccccttccc
     2281 aattcgaagc agcgtttctc tctacggcgc acacatcggc cgacatcgac aagaccatcc
     2341 gcgccgctcg cgtagcattc aagagtttat agttcacgaa agagctgtat taggtcagct
     2401 acagggtgag ctgatttgat accacttgag gccgatcaaa acagttgtcc agcgaggccg
     2461 cagctagcga aggggcgaag cgtacgttgt ctggtacgtt gagcctctga gcgaagcgag
     2521 aacgaagctg gcaactgttt tcatcggccg gtctattcgt cgtcctcgtc gtctgcttcc
     2581 aacgcctcct gcctcttctg ctcttccatc gcattgtgca atagcatgcg aatgaacatc
     2641 gaatacagcg agcccttctc caatgtgccg cccggaggaa tggcgatctt cccttccttg
     2701 atcatcttat ccatccacac cttctgatct ggcgcgatca gaatcgggat ctcgactaga
     2761 ccgacccggc cgaacatttc catcgtcgcc tcctcagcgt gaagcgtcgt tcatctctcg
     2821 tgtctcgcga agaatgaatg aacccttccg agatacgttg tccgagcgac gagatatgtg
     2881 ggtattgcag catgcggttt tcttggaagt caacttcgcg gcgtccgagt tggcacgaag
     2941 cctgcttcag gagtaaacat ggcaccacct cgcgtcttgg atgtaatgac tagcgcggtc
     3001 ttcgctgtct tcggtttgat tggaactgcc cattcccaac catccaccgc ccctccgtcc
     3061 tcgttgtcgg cgactccctt ggagccaaac atgaacaatc cagctggtcc agcgacctgc
     3121 gatctctgcc gagctcttga agggcgaccg ggaagtgatc ttccgcgcca tcggcgcggg
     3181 caacaaaaag acttgcgacc tcacaagcag gcgccggggg taaccaaatc acccaagcga
     3241 agtctcatga gccaactgcc ccaccgcaag ctggaagaga atcatgatca cgaagacaaa
     3301 tgaaggctgg agcagaccgg ttacttcttg tgcttgttgt gcttgctttt tggtccctcg
     3361 gcaaacaaca accaacccag caccacgaac ccaacaacga cgcccgcgat ggccaaccac
     3421 gtcccaatct tatccatggt tggactgtag cgcgacgacc atctccttgt cgaggcgctt
     3481 tacttttccg ttgccgtcgg tgaccgcgag ccgcgccgac ccctcgacga tcacacgtcc
     3541 gctctgccgc tcgcgcacaa cgtgggagaa ggtcatcgaa gccgggctta tgtcgggaat
     3601 catcgtggca atgtccagga tatcgccgta acgggctggt gctcgatagt ccacctctgc
     3661 atgcacgacc acgaacaggc atccttgctt catcaattcc ccaaccgaga gcccgcgcgc
     3721 ttccagatag tgggtcctgg cacgctcgaa atattttaga tagttcgcat agtagacaac
     3781 tccgccgcaa tcggtgtctt cgtaatagat cttgatttcc atagaatcgt cgctcgtatt
     3841 tcgtgaagcg tcactcgcga aaggtgcttg atgagagctt cgttgttatg cactcatggt
     3901 caagccgcgc cggcagctcg tttccatccc acaatctgtg gagtgtcgat attaatcgcg
     3961 gacgcttcga atttttccgt tgatcccatg atgctagagg ttgccgcatg cccacccccc
     4021 gcgacatgct tccgaacgtc gcttcactag acactcagcg tggggaatct tggcaaggag
     4081 gtttcgtcgg catgcgtcac cttcatgacg acgtcgaaca attgctggac ccgttccgct
     4141 ttgaccggtt cgcccccatg gcccagcacg gcacgattcg cggcattgaa caacggtttc
     4201 atcgtcggcc actgacggag aaacgattcg cccatctggt cccccagtcc ggccaagacg
     4261 cggaactgcg cctggagggg cagcacgtat ttgccgtcga tgtcgtccaa ataacaggag
     4321 cggcagacgt cctgcagtgg atgagggagc tgctccggct gcacgtccca ggtcttgatc
     4381 cggtgctgtt tgaaaagctg gcgctgggca aaggcttcga gcgcccggac cagggactgc
     4441 atggctccct ctgcgtcgtt ggccacgcgc agtttccgcc cggcatgtgc cagcaagtcg
     4501 aacgcgagta actctttcac ttccgccggg tcgagcacga gcttttccag gaaaccggcg
     4561 ttggccttta ccgccggaag gcccgcgttt aagcccggcg gtccgccaaa caaggacgcc
     4621 atttcaatgg ccttggtcgc cacttttaac ttttcccagg cctgacgatg ctgaaaccgg
     4681 tcccaaagat catagccttc ggccaggtcc gccagtgcac ggtacagcgg cttctggcct
     4741 ccgctgacac ggctttcgat ccggcgaaag acggcgccgg ctgcaacgaa tgcgccccgg
     4801 ttgaagagat cggatgcctc tcgtctcgcg acttctgcgc cctcatccca agggttgacc
     4861 gaaatccaga ggcgctcacc ggcttcgagt gctgctgggt cgccctcacg gccttcccgt
     4921 tgttcaacca acgagacgat tcgcgaggtc catggcatcg tgatcagggt gagagcgccg
     4981 gccatggctg cggtcgcgcc gctcatatcc acgacaagcg cacccgctcc gatctcccac
     5041 gcccgaagca tggagggaag agagcgggcg agggtctgat aacacgaagg aaagtgatcc
     5101 acatccgaca acggcaccca atcccaccgt cgtggcatgt ggctgatgtg cggttggaca
     5161 tctgtttcca ccagcgcttt cgcggactcc ggcaagacga agcatacgca ttccggacgc
     5221 agtcgattga gcgaatacac gaccgccgag agatcgtcct tcagggcgac gacaagggcg
     5281 gcgactggtt ggtcttgagc catttcgggc ggactataac acgagcatgc gtcgaaatcc
     5341 ctggttcgca gtcacgtgct tgacggtctt cggcaaggcg cttagactcc cgccagcgtt
     5401 ttcattggga ccccatgagg tttctcgatg gtctggtggt attggatggt actcggcttg
     5461 gcgttgctgg gggcggaaat gacgacgccc ggcggatttt acattttgtt cttcggcctc
     5521 tctgctctgg tcgtcggaac cttggccggc ctcgagatgg ttgaggcggc ttggctccag
     5581 tggttgttgt tctctgtgat tgccgtcggc tccttgctga tctttcgcgg tccgttgctt
     5641 tctcggctcc gcgacaatgg ccgcggaggc gcggacatcg actccatggt cggcgaggtg
     5701 gccatccccc ttgaaacgct ggcggtcggc gccacaggca aggccgaact gcgcgggacg
     5761 acgtggagcg caaagaatgt cggcacgaca accttcagca aaggtcagcg ggggaaagtg
     5821 acgaaagtcg aaggcctgac gctttggatc gtggccgaat gagtgaaggc agcgctgtaa
     5881 cgaatatccg acagtcgctc ccggtgaagg gcgagaccaa gcgaatcagc gcggcacgtg
     5941 ggatgccggg atctgagccg cgccgcagcg ggaccgagcg tcggcgcgtg caacaggact
     6001 tatgatggag gaggtcggca tgtcagcagg cacgtgggtg gtcctcgttc tagcgctgct
     6061 cgttctgtac gtgatcgcga agaccgccgt cgtcgtgccg caacaaagcg cctatgtggt
     6121 tgaacggctg ggcaagtatt cggacacgct caacgccggc tttcacatcc tattgccgtt
     6181 tctggactcc attcgatacc gacattctct gaaggaaacc gccgtcgaca ttcccgaaca
     6241 ggtctgcatc actcgcgaca acgtccaagt gtccgtggat ggcgtcctct atctcaaagt
     6301 gctgaatcct gagcgtgcct catacggcat cagcgactac cattttgcct tgtcccagct
     6361 tgcgcagacg accctccgca gcgagatcgg gaagattgaa ttagaccgca cgtttgagga
     6421 acgcaccaac atcaacatcc aggtcgtcaa tgaattggac aaggcatcgg agccctgggg
     6481 cgtgaaggtc ctgcgctacg aaatcaagaa cattacgccc ccgaaggacg tgctcaatgc
     6541 gatggaaaag cagatgcgag ccgagcgcga aaagcgcgcg ctcatcctca cgtcggaagg
     6601 tgaacgcgac gccgccatca accaagcgga aggcgagaaa caacaagtga tcaaggcgtc
     6661 ggaggccaag aaacagcagc aaatcaacga ggcggaaggc gccgcggcgg ccatcctggc
     6721 aattgcgcag gccaccgccg aagggttgcg aaaggtcgcc gataccattc aacttcctgg
     6781 aggccaggaa gcggtgcagc ttcgtgtcgc ggaacaatac atcaccaagt tcggcgaact
     6841 ggcaaagacg aacaacacgc tgatcctgcc ggccagtgtc tcggacgtgg gttcgatgat
     6901 tgccgtagcc atgaatgcca ttcggcagac gacgccgtca gctccgggaa aatcgtaagg
     6961 ccctgcgatc ggattcctga gtcagcgtcc gtctccatcc cggaacctgc gtgtttgaca
     7021 ggatgggacc cctcgcctag aattgcacac gatgaaagac tttgtgatac aagccttcac
     7081 cgacagcgcc gccgtcaagc agcaattcgt gcgcgagtac gcggaccgca tcgtcgaagt
     7141 ggccaagctg ctcgtgagga cattccgtga ggggcataaa gtgttgttat ttgggaacgg
     7201 aggcagcgcg acggatgccg ctcacattgc ggcagaattc gtcggacggt ataaacgcga
     7261 gcgggcgccg ttgcctgcca tcgcgctcgc cacggacgtg gccgcgatca cctgcatcgc
     7321 caatgattat ggatacgacg agctcttcgc acggcaagta cgggctcatg gccaaaaagg
     7381 cgatctcgcc atcgcgatca gtaccagcgg aaattccctg aatgtgttga agggtgtcga
     7441 ggcggcaaag gcgtgcggga tgacgaccgt ggcgtggacc ggcggaaccg gcgggaagtt
     7501 agccgccttg gtggactatc cctttgtcgt gccgtctaag gtgactgcgc ggatccaaga
     7561 aagccacatc accctcgggc acgtgctgtg cgaattgact gaggaagagc tccttggcaa
     7621 agcctcctga ccggataggg cactccctca gcccccgccg cttgatctct ccgatcaatg
     7681 tctctgattt aaaaacttat ccgctcaaga agcgtcacag caaggtgcgc gtctccgatt
     7741 tcgccaagcc ctggcgcaaa ggaggcagct tcagtctgtt ctatcgagcg cttcccgata
     7801 ttcttgccgt caggacgctc cgagctgtgg ccggcgctat tgcccgggca caccgcaaag
     7861 gccggccggt cattgtcggg atcggcgcgc acgtgatcaa ggtcggtctt actccgattc
     7921 tcgtcacctg gatggagcag ggactcgtca ccgccgtcgc catgaacggc gccggcatca
     7981 ttcacgattt tgagctggcg ctcatgggcc atacctccga agaggtcgat gacgagattg
     8041 acgacgggcg gttcggtatg accgaggaaa ccggacgcat cctcaatgaa gccatcgcgc
     8101 aagggcaccg gaacggccag ggaatgggcg aagccatcgg ccactacatc aaccgcttga
     8161 aaagccaatt tcccaaccgg ggaaccagca tcctcgccac cggcgcaacg ttcggcattc
     8221 cagtgacggt ccatgtcgct gtcggaaccg acgtaattca tatgcacccg gcggccgacg
     8281 gggcagcgat cggcgccacc tctcttctgg acttccggcg gctcgcggcg gtggtcgccg
     8341 gcatggaagg cggggtctat ctcaatatcg gttcggcggt cgtcctgccc gaggtgtttc
     8401 tgaaaaccgt gtcgctcgga cggaatctcg gccatacctt atcgaagatc accaccgtga
     8461 acatggactt cctgtctcat taccgcccgc tcaccaacgt cgtccgccgg ccgactcaaa
     8521 aaggcggcaa ggggtattcg ctgatcggcc atcacgaaat catgttaccg ttactggttg
     8581 cggccgtcgc agaagagctg ggataactca gaatgacagg tcatgccgac ccgctcagcg
     8641 tctcacgcat cacggcaatc tggtgcgacc tgaataatcg atattttggc ggcgcgcttc
     8701 cgcccatcgc ggtcgtgtgg agcaggcggc tgactgcgtc cgtcggcatg ttcgtgagtc
     8761 gagccggtcc acgctcggca tggcgtgcga acgcatcccc caaaagccgc gagattcgcc
     8821 tctcccttcc cctgttgaca cccttgctcg cgggcacgcc ctacggcacg caggaactcg
     8881 tcagcaccct cgcacatgaa atgatccacc agtggcaatt cgacgtgctc aagcggcggc
     8941 caaatcacgg gcccgacttt ctcagaaaga tgacggagat gaaccgcgac ggcgctcttg
     9001 ctgtgaccat ctaccactcg ctcaagcagg aagtgcttgc actgtcccgg tttgcctggc
     9061 gttgcggacg ctgtgggcgc atctaccgcc gtcagcggcg gacgatccag ccgcggcgcc
     9121 accattgcgg cagctgccgc ggtgcgttgt atgaattgtc ctcatcggag gccggccagc
     9181 cttcatcctc ggaaacggat aggttgaggc ccccctgcac gtcgatacaa cctgcccgac
     9241 agcttgagct ggatttttcc tccgcaagcc tctaggctct gtttgcactg accgcccact
     9301 agacggtgtt cgatgctgcg atggccgcgc cagattctat ttggtgacgt cgacgcgatg
     9361 tttgcatctg cggccaccgt caaagatccg tccctggcag ggaagccgat tgccgtcgga
     9421 ggcccgccgc cccgaggcat catcgcggcc gcaagttacg ccgtgcgggc cttcggtgtt
     9481 cgatcggcca tgccgacggc ggaggccctg cgacgatgcc ctcacctcca gctgatcccg
     9541 ccagaccggc cgctgtatcg tcgtctccat gacgaaatgc gcagggtcac ggaccggttg
     9601 ttccccgtga ccgaatgggg cagtatcgat gaattctacg ccgacacaac ggacctccaa
     9661 acgcgccatc cggatccccg actgctcgcg caactggtga aggactctat tttcaaggcc
     9721 accggcctgc gatgcaccat cgccgtggcc acggggaaaa ccgttgcgaa gattgccgct
     9781 gatgcgtcga agcccgacgg tcttgccatc gtcgaaccgg accgggaagc ggcgtttctg
     9841 ctgccacggc cactgcggtc tcttcccgga atcggaccaa agaccgccgc gcgcctcgag
     9901 cccttcagca tcaagacgat cagcgatctc ttggacccac ggtgggatca ggtgctcgcg
     9961 aggatgttcg gcgcgcgtct tttgtggatt cgagagctgg cacagggccg cgatcgggaa
    10021 ccggtcatcg gtgaccggga atccaagagt tcgagccacg agaccacatt cgagcgggat
    10081 actgctgatc cgacattcct cgaagacgtt ttgaggggat ttttgcgcat gctctcgcgc
    10141 gacctgcgcc aggaggggct ggcggcggaa ggatgcaccg tcaaattaaa ggatgcccgc
    10201 tttgccgtga cgaccaggca gcgacggttt ccccatccgc tcaattacga tcccgaaatg
    10261 tggccgacgg tccgacaggc attgcacgaa cttatcaagc cgcacgcaca ctatcgcttg
    10321 gccggcctca cactcttttc actcgtcccg gtccctgccg gcctgtacga ccagcgccgg
    10381 cgcaaagctg tccaagccat ggacgccatc atcgccaggc atggtgcagt gatcggtttg
    10441 ggcggcgtgt gtgacgatga caatgagtag tccgccaagc ggctttctat ctgtccatac
    10501 tgtcggtatg atggaacgat gtcgggcaaa gtcgtgacga gtgctgaatt ggttcctttc
    10561 ctcaaacggg cgcgcgccca aaagcagcgc gtcgttttca ccaacggctg cttcgacctc
    10621 atgcacatcg gccatacgcg gtatctgcag gcggccaaag cgcttggcga catcctcgtg
    10681 gtcggcgtca acagcgatgc gtcggtccgg agcctgaaca aaggtaccga tcggccgatt
    10741 gtcggtgagg ctcagcgggc cgaggtggtt gccgcgatgg gcgcggtgga ctatgtcgtc
    10801 atctttcccg aacccgatcc tctggctttg atcacgacgc tcgaaccgga tgtgttggtc
    10861 aagggcggcg attggaccct cgagcgcatc gtcggacgcg aagtcgtcga acaacgaggc
    10921 ggaacggtca agacgattcc actggtgccc ggcatgtcaa cgaccgcact catccagcga
    10981 atccggtctc aaccacactg agtcctgtgt cacacgccct gtcgagctca acaacgctcc
    11041 cctgttgcgc gccgattgcc gctgccctag ccagaaaggg cgcacggccc tgcttaaccg
    11101 gattgcatgg ttccaccgca gggttcgccc tcacgcaact catgcaccct cgagccggga
    11161 cgctcggctc cgaaccctgg ttgatcgtga ccaagacgga cgaagccgcc gagcggttgt
    11221 tcgaggacct ccgtttctat catggtctcc acggagtgcc tgttacggct ctcgcactct
    11281 tcccccggtg ggaaaccctg ccttacgaat cgaccgctcc tcatgtcggc ctggtcgcgc
    11341 gccgtatgaa cgccctgcac cagattcgat cgaggcctgc cgccaaggtg gtgacttcga
    11401 tcgaggccct gatgcagcgg ctcatgccga tggacacttt cctgcgtaca gtgttgcagt
    11461 tcaaggtggg agccggtatt gaacgcgaag tattgacggc tggccttctc aggctcggct
    11521 atcgtcgggt gtctgtcgtt gaaattccgg gtgaattcag catccgcgga gggatcgtgg
    11581 acatcttctc gacggcgtat gctgaaccgt tgcgcgttga gttcttgggc gaaacggtgg
    11641 aatctctccg actgttcgat cctgccacac agaaatccac ggccaaaatg gaccgggcca
    11701 tggtcttacc cgcccgtgaa tacctgaaac cggacaccgc cgccgatgcg ctcgcgccaa
    11761 tctccgctga cgccgaatgg cgcgcacccg atctctatgg ccaaatgcac agtctgctcg
    11821 actacttcac gacgcaacca atcctcgcgt tggaccagcc tgcagccttg aagcagtcct
    11881 gtgcggacct ctgggagcgg atcgacgacg gttatctgag gcacagcgac aaagaggaag
    11941 tgtcctatcc ctcacccgaa cggctgttcc ttacttggca gaacatcatg gaccgcacgg
    12001 ccgaatgcac ccaactggcg ctcgaaccgc tggcagaagc cgaaggctcg tgggaaccgg
    12061 tccaggcatt ccccggtcag gtaccggcca gcgccggctt gggcgcgcgt ggcacgccgt
    12121 tcagccagac cctgtcgatc cttgagcacc tgcgggaggc ctgtcgggtc ttgctcgtgg
    12181 cgagaagtcg cggccaggtg gatcgcctgc tggcacttct gcgcgagcat gacgtgccgg
    12241 cgattgaatg gtcggcgtca agctggtcta cgtcgacaga gacgcgcgcg ccgttttttg
    12301 tcctcaacgg cgatctttcc gcgggctttc tgtcgtcgga actccggctg gcaatcctca
    12361 cggaggaaga actgtttgca aagggcgccc gtcacaagcc gcaggccaag agcaaggctg
    12421 cgacgttcct gtcgtccctt gaagatctca atgtcggcga ttacgtcgtg cacgtccagc
    12481 atggtatcgc taaatatcgc gggctgaagc gtctgtctgt ccaggacttc gacagcgatt
    12541 acc
//
LOCUS       DASTQY010000414         5399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1504302, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000414 DASTQY010000000
VERSION     DASTQY010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504302"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VFR82_13435"
     CDS             <1..673
                     /locus_tag="VFR82_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502650.1"
                     /translation="LPADPIAAMGGTITGGPTGVPMRQTLNLASKIKVLGVVVGDRDG
                     VSAIIEELGSKRQLFLRLHDEIPDIGELSEIRRNGIVVRQGNQQEFLELSTGQERPPM
                     PQPVSTGPATSGGGGQIKKVLDRREVEQALNDLPKLLSQARAVPVLSNGAMTGFRLDY
                     IAPSSFYEKIGLHYGDVLKQVNGVEIKDPGTMLSLFQQLRNEKSVKLDVLRNNQRTTM
                     LFEIR"
     gene            864..2594
                     /gene="gspE"
                     /locus_tag="VFR82_13440"
     CDS             864..2594
                     /gene="gspE"
                     /locus_tag="VFR82_13440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEU4502651.1"
                     /translation="MAKHTTPTRPLLGAILEQKFGLSEAKLLEALNIQQTKGGRLGEA
                     LIKIRAIEEDLLLQALAMQFEMTWLSHLDVKQVDQELIKKVPIHFARRYRVLPLKVDD
                     GAIVVATIDPLETAALDDLRLLLGMPVKPVLTGAMSLMACLNRAYDEAASPTGAEQVM
                     EDIAASENLDKIAHELDEPQDLLDATDEAPIIRLVNSVLFQAVRQRASDIHFESFERG
                     LVVRYRIDGVLYPILTPPKHLQSSIIARLKIMAGLNIAEKRLPQDGRFAIRTAGKDVD
                     LRVSVLPTSHGERVVLRLLEKENRLLNLSEMGFSPDRLRTIQQLIRLTHGILLVTGPT
                     GSGKTTTLYAALSEINAPDKNIITVEDPVEYQLLGIGQMQVNPKINLTFAAGLRSILR
                     QDPDVIMIGEIRDRETAEIAIHASLTGHLVFSTLHTNDAASAATRLIDMGIEPFLVAS
                     SVVAVLAQRLIRKVCPDCKQAYHPDDEELIRLGVVPGKTKPTFYRGTGCPACTQTGYR
                     GRTGIHELLVLDDEVRRLIGNKADAAVIRQAAVSKGMVLLKEEAAEKIFAGITTTEEV
                     MRMTQQEVEV"
     gene            2598..3836
                     /gene="gspF"
                     /locus_tag="VFR82_13445"
     CDS             2598..3836
                     /gene="gspF"
                     /locus_tag="VFR82_13445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEU4502652.1"
                     /translation="MPVYQYRGYKADGASATGIVDAESPKVARLKLRKDGVFPTEMAE
                     QEGATKTVIARSSSGLIAGVTRSPVLTVTDVALMTRQLATLLVAGLPLVDALGVLMDQ
                     TEKKAVKGLLADVREAIRGGASYSAVLERYPRDFSSIYVHMVRAGEASGALDQILFRL
                     AEFLEKQLALKNKVTNAILYPAIMLVVGTLVLFFLVTFVVPKITAVFAHSKQALPWPT
                     IVLMEISQFCSDYWMVLLAGILAGIWGLRRFKKTETGRVFADRLLLKLPLIGQVARMV
                     SISRLASTLATMLSSGVQLLEALDVARRVMNNRVLEQAVEEARQNIREGETIAEPLKR
                     SGQFPPLVTHMIAVGERSGEMEEMLRRIGQIYDGEVDRVITRFTSLLEPIMIVVMGVV
                     VLFIVVAILLPIFEMGQMVR"
     gene            3861..4361
                     /gene="gspG"
                     /locus_tag="VFR82_13450"
     CDS             3861..4361
                     /gene="gspG"
                     /locus_tag="VFR82_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEU4502653.1"
                     /translation="MRAQTHTVRGGTAVFRSERGFTFIEIMVVVAILAILAALVVPRI
                     MGRTDDAKRTAAKVQIRNIEGALQLYKLDNGVYPSTEQGLRALVEKPSVGVVPKKWKL
                     GGYLPSIPEDPWGNPYKYLSPAPVQQGQYGQIKADYEITSYATDGEAGGEGVNADITN
                     WNMEKD"
     gene            4366..5034
                     /locus_tag="VFR82_13455"
     CDS             4366..5034
                     /locus_tag="VFR82_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502654.1"
                     /translation="MEPRARLELPTRGTSESALGQASIAKFRRLHDASGFTLLEIVMV
                     LFLLTGMLSLVIPKLSIGQNLGSVGRKWVGSLKMLQDLSMTTQKTVRLYVDIDRGMYW
                     PMVLDGNEEKTPQDPIWAYPLSLPETVRFSDVQVGATRKETGRVDIFFYPNGRIDQAT
                     VHLTDGNNNVMGIFVEPVTALIRVTDHRIDPPKPLAILERIRPLLQPITPGLRPASVM
                     GAPK"
     gene            5067..>5399
                     /locus_tag="VFR82_13460"
     CDS             5067..>5399
                     /locus_tag="VFR82_13460"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU4502655.1"
                     /translation="MPHQHVSRHSPAKANGFTLLEVLIAIAILAFALPVLLGLRNRDL
                     DFHARAADITAATMLAQEKLIEAELSPVYPLGETTGDFRNPPPGYQVPGDIANRAQRF
                     RWKRLITST"
BASE COUNT         1184 a         1502 c         1610 g         1103 t
ORIGIN      
        1 acttccggca gacccgattg ccgcgatggg agggaccata acaggcggac caaccggcgt
       61 acctatgcgc cagaccttaa accttgcttc caagatcaag gtgcttggcg tagtggttgg
      121 agaccgtgat ggggtatcgg cgatcattga agagcttggc agcaagcgcc agctgtttct
      181 ccggctccat gacgagattc cggacatcgg agagctgagt gagatccggc gaaacggcat
      241 cgtggtccgg caaggcaatc agcaggaatt cctggaactg tcgaccggac aagagagacc
      301 tccaatgcct cagcccgtgt cgaccggacc ggccacgtct ggcggaggag gccagattaa
      361 gaaggtcctg gatcggcgag aagtagaaca ggctttgaat gatcttccca agctgttgtc
      421 tcaagcccga gccgttccgg tgctttcgaa tggggcgatg accggatttc gcttggacta
      481 cattgccccc tccagcttct acgaaaagat cggtttgcac tatggggatg tgctcaagca
      541 ggttaatggc gtagaaatca aagaccccgg cacaatgctc tcgttattcc aacaacttcg
      601 taacgagaaa tccgtcaagc tcgacgtcct acgaaacaat cagcgtacga cgatgttgtt
      661 cgagatacgc taagtcagcc gggctgacat ccttccgacc ccctcttgtt cactgggtat
      721 atctcctggt ttggctgcca gtcagccggc agcttgtcag ggccggtcgg agaggagtag
      781 tttagttctt tcggaggctt aggctggttt aagcaatcca caggggtagg agatccccta
      841 gagatataga ggtctgacca ctcgtggcga aacacacgac tccgacgcga ccgctactgg
      901 gcgccattct ggagcaaaag ttcggtttat ccgaagccaa gctcctcgag gcgctgaaca
      961 tccaacagac caagggggga cgtctgggag aggccctcat caagatcaga gcgatcgaag
     1021 aagatctgct gctgcaagcg ctcgcgatgc agttcgaaat gacctggctt tcccacctcg
     1081 acgttaagca agtcgatcag gaactcatca agaaggtgcc gattcatttt gcgcgccgct
     1141 atcgggtgct gcctcttaaa gtggatgacg gagcgattgt cgtcgcgacc atcgatccct
     1201 tggagactgc cgctctcgat gatttgcggc ttctcctggg gatgccggtg aagcctgtgc
     1261 tcactggcgc catgtccttg atggcctgtc tcaatcgcgc gtacgacgag gccgcgagtc
     1321 caacgggggc ggaacaggtg atggaggaca tcgcagccag cgaaaatctc gacaaaatcg
     1381 cacatgagct ggacgaaccc caggatctgc ttgatgcgac ggacgaagcg ccaatcattc
     1441 ggttggtgaa ctcggtgctg tttcaggcgg tccggcagcg ggcgagcgac atccacttcg
     1501 aatcgtttga acgcgggctc gtcgttcgct atcgcatcga cggcgtgctc tatcccattc
     1561 tcacgccgcc gaagcatctg cagtcgagca ttatcgcccg tctcaaaatc atggcaggat
     1621 tgaatattgc ggaaaagcgg ctgcctcaag acggccgctt tgccatccgg actgccggaa
     1681 aagatgtgga cctccgcgtg tcggtgttgc cgacatcgca cggcgagcgc gtcgtgttgc
     1741 gcctgcttga aaaggaaaat cgactgctta atctgtcaga aatgggcttc tcgccagacc
     1801 ggcttcgcac gattcaacaa ctcattcgtc tcacgcacgg gatcctcctc gtgacgggac
     1861 cgacgggcag cggcaaaacc acgacactgt atgcggccct gagtgaaatc aacgcgccgg
     1921 acaagaacat cattacggtc gaagacccgg tcgaatacca gttgcttggc atcggccaga
     1981 tgcaggtgaa tcccaagatc aatctgacgt ttgcggccgg gctgcgatcg attctccggc
     2041 aggatcctga cgtcatcatg atcggtgaaa tccgtgaccg agagacggcc gagatcgcca
     2101 ttcatgcgtc tctgaccggt cacttggtct tctccacact gcataccaat gatgccgcaa
     2161 gcgcggccac ccgtctgatc gacatgggta tcgagccgtt ccttgtcgcc tcctcggtgg
     2221 tggcggtcct ggctcagcgg ctgatccgaa aggtctgtcc cgattgcaaa caggcctatc
     2281 accctgacga tgaagagctc atcaggctgg gagtggttcc cggaaagacg aagccgacgt
     2341 tttatcgtgg caccggctgt ccggcctgca cgcagaccgg ctaccggggt cggacaggga
     2401 tccatgaact gctcgttctg gacgacgaag tccgccggtt gatcggcaac aaggcggatg
     2461 cagccgtcat cagacaggcg gcggtttcga agggcatggt cctcctcaag gaagaagccg
     2521 cggagaagat ttttgccggc attacgacga ccgaagaggt catgcgcatg acgcaacaag
     2581 aggtcgaggt ctagcggatg ccggtctatc aataccgggg ctacaaagcg gacggtgcct
     2641 ctgctaccgg cattgtcgat gccgaaagcc cgaaagtcgc ccgactcaag ttgcggaaag
     2701 acggcgtatt ccctaccgaa atggcggagc aggaaggcgc gaccaaaacg gtcatagccc
     2761 gctcctcttc agggttgatc gccggtgtca cccgctcccc cgtgctgacg gtgaccgacg
     2821 tggccttgat gaccaggcaa ctggccaccc tcctggttgc gggtctgccg ctcgttgacg
     2881 ccctgggcgt gctcatggac cagaccgaga agaaagcagt gaagggcctc ctcgcggacg
     2941 tccgggaagc gatacgcgga ggggcatcgt acagcgccgt actcgagcgg taccccagag
     3001 atttttcttc catctatgtc catatggtgc gggccgggga agccagcgga gccttggacc
     3061 agattttgtt ccggctagcg gagttcctgg agaagcagct ggctctcaag aataaagtga
     3121 ccaatgcgat tctctatccg gcaatcatgc tggtggtcgg gaccctggtc ttgttctttc
     3181 tcgtgacgtt cgtggtcccg aagatcactg cagtctttgc ccattccaaa caagccctgc
     3241 cctggccgac tatcgtgttg atggaaatta gtcaattttg ctccgactac tggatggtcc
     3301 tgcttgcggg gatcctggcg ggcatctggg gactccgccg gttcaaaaag acggagaccg
     3361 gtcgtgtctt cgccgaccgg ctgctcctca agcttccgtt gatcggtcag gtggcgcgaa
     3421 tggtctcgat ttcgcggttg gccagtacgt tggccacgat gctgtcgagc ggcgtgcaat
     3481 tattagaagc gctcgatgta gcgagacggg tcatgaacaa ccgcgtgctc gagcaagccg
     3541 tcgaagaggc ccggcagaac atccgggagg gagaaacgat cgcagaaccc ctgaaacgca
     3601 gcggccagtt tcctccattg gtcacccaca tgatcgccgt cggtgagagg agcggagaaa
     3661 tggaagagat gctgcggcgc atcgggcaga tctatgacgg cgaggtggat cgcgtcatta
     3721 cccgctttac gtcgctgctc gagcccatca tgatcgtcgt gatgggtgtg gtcgtgttgt
     3781 tcatcgtcgt cgcgatcttg ctccccatct ttgaaatggg acaaatggta cggtaaacgt
     3841 tgctatctga ggaggtatcg gtgcgcgcac agacacacac ggtacggggc ggcacagcag
     3901 tgttccgatc ggagcgcggt ttcacgttca tcgaaatcat ggtggtcgtg gcgattctcg
     3961 ccatcctggc cgcactagtg gtgccacgaa tcatggggcg aacggacgat gccaagcgga
     4021 cggctgcgaa ggtccaaatc cgtaatatcg aaggtgcact ccagttgtac aaactcgaca
     4081 acggtgtcta tccgtcgacc gagcaaggtc tcagagcctt ggtcgagaaa ccttcagttg
     4141 gtgtcgtgcc gaaaaaatgg aagctcggcg gatacctgcc aagtattccc gaagatcctt
     4201 gggggaatcc atacaagtat ctgagcccgg cgcccgttca gcagggacag tatgggcaga
     4261 tcaaggccga ctatgaaatt acgtcgtacg cgacggacgg cgaagccggc ggagaaggcg
     4321 tcaacgccga tatcacgaat tggaatatgg agaaggacta agatcgtgga gccgagagcc
     4381 aggttggagc tgccaacgcg tggtacgagc gagtctgcgc tcggtcaggc ttccatcgcc
     4441 aagtttcgca ggctacacga tgcaagcgga ttcacgctgc tggaaattgt catggttctg
     4501 ttcttgttga ccgggatgct cagtctcgtg attcccaagc tgtcgatcgg gcaaaatctc
     4561 ggcagtgtcg gaagaaaatg ggtaggatct ttgaaaatgc ttcaagactt gtcgatgacg
     4621 acgcagaaga cggtgcgatt gtacgtcgac atcgatcgcg ggatgtactg gccaatggtt
     4681 ctcgatggca atgaagaaaa gactccgcaa gatccgattt gggcctaccc gctcagcctt
     4741 ccggagaccg tgcgattctc agacgtccaa gtgggtgcaa cgagaaagga gaccggccgc
     4801 gtggatatct ttttctaccc gaacggccgc atcgaccaag ccacggtgca tctgaccgat
     4861 ggcaacaaca atgtgatggg tatttttgtg gaaccggtga cggctctgat ccgcgtcacg
     4921 gatcaccgta tcgatccgcc gaaaccgctg gccatcctcg aacggatccg gcccttgctc
     4981 cagcctatca cgcccggcct caggccggcg tccgtcatgg gcgctccaaa gtagcgcatt
     5041 ccaggaagct gaacacggag ggggtcatgc cacaccagca tgtgagccga cattctcccg
     5101 ctaaggcaaa cggcttcacg ctgctcgaag tgctgatcgc gatcgcgatt ctagcatttg
     5161 cgctgccggt tctgctggga ctgcgcaatc gggatttgga tttccacgct cgggcggctg
     5221 acatcaccgc cgcgacgatg ttggctcaag aaaaactgat cgaagcagaa ctgagtcctg
     5281 tctaccctct cggggaaacc accggtgatt ttcgaaaccc gccaccgggc taccaggtac
     5341 cgggcgacat cgccaatcgt gcccaacggt tccggtggaa gcgcctcatc acgtcgaca
//
LOCUS       DASTQY010000415         3759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1727393, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000415 DASTQY010000000
VERSION     DASTQY010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727393"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VFR82_13465"
     CDS             <1..457
                     /locus_tag="VFR82_13465"
                     /inference="COORDINATES: protein motif:HMM:NF017013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HEU4502656.1"
                     /translation="GMRTVLLPPLIEEARMSGAYVGSLAHSILTKQEPIPRPCCLVAG
                     GETTVNVRGAGKGGRAQEFAVAAAQVVAGLPDVYVAGVGTDGTDGPTDVAGALVDGNT
                     WQRARRLGIDLNAALKQNDTYRALKRLGSHIITGPTGTNVNDLYLLFVL"
     gene            472..3090
                     /locus_tag="VFR82_13470"
     CDS             472..3090
                     /locus_tag="VFR82_13470"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEU4502657.1"
                     /translation="MGAPLVLPLAQCRDASLAGGKAAGLARLLKTGVKVPPGYCLTTC
                     AYRAALEAVGFSPGECWRRAIKLQGDDRERELEDCRQMILRADITHLVHDLRQTLGEG
                     IGFEQRWAVRSSATNEDMVRMSYAGLYRTELGLLWDEIGRGITAVWVSVWDAGVIEYM
                     KKSGTAEAPPAMAVIIQPMLDAAVAGVAYSVDPVTGRSGHVTVNAIRGLGSPLADGKV
                     TPDQYTVETGPDQPARLIRYTPGHQAERLLLGRSGVTTESVRAEERNVSALSEQQLVE
                     VAALVNRIEQAFGGPVDVEWAFDQQGLWVLQSRPVTAVRSVSDLTNEESEWSRANFKE
                     TLPEVPSPMGLSFLEQFMDAYILSHYRRLGCRIPSDVSSVRTLSGRPYVNVSLFYTLI
                     GQLGGNPALLVEQMGGEPLQSPVMVQPLDWPAFLKAGFLMWQEMRRVLQCSPTWFAEM
                     KALALLYSPDRVRALSLEDAEARLDELARWLPSREVTFGIAAGVGQSLQAFSRLLPGW
                     LGRDWRGLLNAALQGLGTVISAQQILRLAELVELARAEPAVSDRLSRGGWERGFYRRS
                     FAGSEFLAGFDRYLADYGHRGIGESDVMSPRMADQPEALLEVIRIQLAGPPTTPAAIA
                     GRQQAAREEALASIKARCGLRFDRWLIFHWWYRRLCRFFALREANRHHLMWYSAAARN
                     LLLHVGDLMTVQGLFHVPGDIFFLTLQECEVMSSERRKNWGALVKARRAQREQWLSVE
                     VPDTIRNLEPSELSDPLLEPNGLLSGIPVSSGRASGPVTFVRSEADWINVRRGDIIVA
                     PVIDPGMAPLFGVAAGLVVEMGGTLSHGAIIAREYGLPTITNVRRVMTQLSQGEIVTV
                     DARHGIVRREAAASGP"
     gene            3355..3645
                     /locus_tag="VFR82_13475"
     CDS             3355..3645
                     /locus_tag="VFR82_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502658.1"
                     /translation="MLENPAFGLGLLGGIVLGGLAVGAWISARLRARFDGELCHALAR
                     AQFAETLTEELRRGEQVDQAEVGRLRGELADAVRARAIAETQAEETVKRAEE"
BASE COUNT          708 a         1047 c         1205 g          799 t
ORIGIN      
        1 gggaatgcgc accgtccttc tccctccgct catcgaggaa gcccgtatgt ccggcgccta
       61 tgtgggttcg ctcgcacaca gcattctgac aaaacaggaa ccgattccga gaccgtgctg
      121 cctcgtggca ggaggcgaga caacggtgaa tgtcagggga gccggcaaag gcggccgcgc
      181 gcaggaattt gcggtagcag ccgcccaggt ggtggccgga cttccggatg tgtatgtggc
      241 cggcgtgggc accgatggca ccgatggtcc gacggatgtt gccggagctc ttgtcgatgg
      301 aaatacgtgg caacgagcga ggcggttagg gattgatctc aatgccgcac tgaagcagaa
      361 cgacacgtac cgtgcgttga agcggctcgg gtcccacatc attacgggtc caaccggcac
      421 caatgtcaat gacctttacc tgctctttgt cttgtaggta ctatccgact gatgggtgct
      481 ccgctcgtgc tgccactcgc ccaatgccgt gatgcctcgc tcgccggcgg caaggccgcc
      541 ggtctcgcgc gcttgttgaa gaccggagtg aaggtgccgc cggggtactg tctgacgaca
      601 tgtgcctatc gtgccgcgtt ggaagcggtc ggattttctc caggtgaatg ctggcggcgg
      661 gcgattaaac tccagggtga cgaccgggaa cgggagctcg aagactgccg gcagatgatt
      721 ctgcgcgcag acattaccca cctcgtgcac gacttgcgtc aaacccttgg ggagggaata
      781 gggtttgaac agcggtgggc agtacgttcg tcggcaacta acgaagacat ggtccggatg
      841 agttatgccg gcctgtatcg aacggagctc ggactgctgt gggatgagat cgggcgcggc
      901 atcacagcgg tctgggtctc cgtctgggac gcgggggtga tcgagtacat gaagaagagt
      961 ggaactgccg aggcgccgcc ggccatggcg gtgatcattc aaccgatgct tgatgcagcg
     1021 gtcgccggag tcgcctattc ggtcgatccg gtgacggggc ggagcggtca tgtgaccgtc
     1081 aacgcaattc gtgggctggg atcccctctt gccgacggaa aggtcacgcc ggatcagtac
     1141 acggttgaga cagggcccga tcagccggct cgactgatcc ggtacacccc cggtcatcag
     1201 gcggagcggc tattgctcgg tcgcagcggt gtcacgactg agtcggttcg tgccgaggag
     1261 cgcaacgtat ccgctctctc ggagcagcaa ctggtcgagg tggctgcgct ggtcaaccga
     1321 atcgaacagg cgttcggcgg accggtcgac gtcgaatggg cgtttgacca gcaagggctg
     1381 tgggtccttc aatcgcgacc ggtcacggca gtccgatcag tctctgatct caccaatgag
     1441 gagagtgagt ggtcgagagc gaatttcaag gaaacgctgc cggaggtgcc cagtccgatg
     1501 ggtctttcgt ttcttgaaca gttcatggat gcgtacatcc tttcccacta tcgccggctg
     1561 gggtgtcgaa tcccgtccga tgtttcgtcc gtccgtacgc tgtcaggtcg gccctatgtg
     1621 aatgtgtctc tgttctatac cttgatcggt caactgggtg gcaatcccgc cctgcttgtg
     1681 gaacagatgg gaggcgaacc actgcaatcg cctgtgatgg tgcaaccatt ggactggccg
     1741 gcattcctca aagccggttt tctcatgtgg caggagatga gacgagtctt gcaatgtagt
     1801 cccacctggt tcgcagagat gaaggcactc gctctccttt atagtcctga ccgcgttcgg
     1861 gctctatccc tggaggatgc cgaagctcgc ctcgatgagt tagctcgctg gctgccttcc
     1921 cgcgaagtga cgtttggaat tgcggcagga gtggggcaga gtttgcaagc cttcagccgg
     1981 ctgctgcccg gctggctggg aagggattgg cgcgggttgt tgaacgccgc cttgcagggt
     2041 ctaggcaccg tcatcagcgc gcaacaaatt ctacgcctgg ccgagttggt cgagttggcc
     2101 agggctgagc cggctgtgtc ggaccggctc agccgtggag gatgggagcg aggcttttat
     2161 cgtcgaagct tcgcagggag tgaatttctt gccgggttcg accgctacct tgcggactat
     2221 ggtcatcgcg gtatagggga atcggacgtc atgtctccgc ggatggccga tcagcccgaa
     2281 gctctgctcg aggtgatccg gattcagctc gccggtccgc caaccactcc ggcagccatt
     2341 gccggacgcc aacaggcggc gagagaagag gcattggcgt cgatcaaggc tcgctgcggt
     2401 cttcggttcg atcggtggct catttttcac tggtggtatc ggcggctctg tcgattcttc
     2461 gccctgcgcg aagccaatcg ccaccatttg atgtggtatt ccgctgcggc gcgaaacctg
     2521 ttgcttcatg tcggagatct catgaccgtc caaggcctgt tccacgttcc aggggacatc
     2581 ttctttctga cgctgcaaga atgcgaggtc atgtcatcgg aacggagaaa aaactgggga
     2641 gctctggtca aggcacgccg agcccagcgg gaacagtggc tgagcgtgga ggtaccggat
     2701 actattcgga acctggagcc atcggagctg agtgatcctc tcctggagcc caatggcctg
     2761 ctctcgggca ttcctgtcag ctccggacgg gcgtccgggc cagtgacctt tgtgcgatca
     2821 gaggcggatt ggatcaacgt gcgacgaggc gacatcattg tcgcaccggt catcgatcct
     2881 ggcatggccc cattattcgg tgttgcggca ggattggtcg tggaaatggg cggcacgctt
     2941 tcacacgggg cgatcatagc ccgcgagtac gggttgccga cgatcaccaa cgtgcgtcgc
     3001 gtcatgactc aactgtccca gggcgagatt gtcaccgtcg acgcccggca tggtatcgtg
     3061 agacgtgagg cagccgcgtc gggaccctaa cagaccagtc gacaagaaac ttgctgcgct
     3121 cgttggacat ccgcgctcgt ccggtttgca ggcaagactt ttttgtcaac aaccctaagg
     3181 gaattttcct gatgcagacc tctcgtctgt agggccgaac ggctctgagt gttttcctgc
     3241 tcttgacgtt tttcgtgata ttacgtaacc tcccgcaact aatcgttcaa ttgcctgtcc
     3301 atacatccat actgggatca gaacttctct ccgtgcatgg acggagtccg acctatgctt
     3361 gagaatccgg cgtttggcct cggcctcctt gggggcattg tgctcggagg actggctgtg
     3421 ggcgcgtgga tttctgctcg cctgcgggcc agattcgatg gcgaactatg ccatgcgtta
     3481 gcacgtgccc aattcgccga gacgttgacc gaggaattgc ggcgtggtga gcaggtcgac
     3541 caggcagaag ttggccgtct ccgcggcgag ctcgccgacg ccgtgcgagc ccgcgcgatc
     3601 gctgaaacgc aggcagagga aaccgtcaaa cgtgcagagg aataacagac actcctggcc
     3661 aaggcgcgtc acgagctggc cgaatcattt caagccctct ccggagaggc actcaaacag
     3721 aataatgagg catttctcac gctcgccaag agcacgttt
//
LOCUS       DASTQY010000416         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_296996, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000416 DASTQY010000000
VERSION     DASTQY010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296996"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..208)
                     /locus_tag="VFR82_13480"
     CDS             complement(59..208)
                     /locus_tag="VFR82_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502659.1"
                     /translation="MPDYRQPIQEYMRACEALLKIELTNEETEAVEEMYGRIADKFID
                     AGRLL"
     gene            complement(687..1448)
                     /locus_tag="VFR82_13485"
     CDS             complement(687..1448)
                     /locus_tag="VFR82_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502660.1"
                     /translation="MNPKTLRRLTFLCCATISILTAATGCSRWIEMAPLSETQDPGAQ
                     QIARDEQVPLVIDVIRTTRNGSPQNTSAETQQRILSSLAEIGLFSHLSSGTYAVSASN
                     DKIVRAKILLSETIDPHAGGAAWKGIVIGASMYTLTPFLPLEYGYAANVSLELERWDG
                     QVKRYEGQSSGTARYQLFGATPIMIEELKGHVTETCLNELARQIVRDTAFYTASSAPA
                     SHSDIRTVTIRRRSPQIPDASSTPIPVTVSAPADR"
     gene            complement(1458..2063)
                     /locus_tag="VFR82_13490"
     CDS             complement(1458..2063)
                     /locus_tag="VFR82_13490"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEU4502661.1"
                     /translation="MTPPSLAARIAFALLVSTMTIPCQAGEVTPHLTVGTQEISLSAG
                     YLLPHRLTTEHTTKQSGPALMPSWAMVLTDPIGKSFYRGQLLLGAEIVYIQFQEPFLT
                     HGVGFTPKIKYMFEGTGRLRPYIEFAGGPFYTDLGGRIPEESSRFNFILTAGGGLSWF
                     FTSQFSFNVGYRFHHISNAGTRMPNLGLNSSLPFGGFSFYF"
BASE COUNT          523 a          538 c          716 g          529 t
ORIGIN      
        1 cggtcgcaag aggccgcagg cgaatgtgga gatttaaaag tgtggaatca ggccagtttc
       61 acagtaatct acccgcatcg atgaatttat cggcaatccg accatacatt tcctccacgg
      121 cttcggtttc ttcgtttgtg agttcgattt ttaacagggc ttcgcacgca cgcatgtatt
      181 cctgaatggg ttggcggtag tccggcattc gactcctttt gggggtacgg aagttcagga
      241 aacgaaatgg tgtttacttc tcggaggaac cattgtggat cttttggact tcagccacat
      301 aatacttaat catcgccagt tcctcctcag agaacgctgg gctggaggtt gcggctgcta
      361 agagacgctc actagctcgg aggtactcct gaacttcgcg acgataggcg aagacaggat
      421 tgtccgacat agaacccgac ggcctttctc acaagtggcg ccggtcgggt tttcctggag
      481 accgtcggca gtgtgtgtgg ttggaggcag gagtatgccc tttcttgggg ggggcaataa
      541 gctattagct ttaggataaa gtatttagca tcaatttaag ttgggacgcg ataggagatg
      601 gtgtcaaggt ggttcgacgg ggccaggaag gattgaagac ttggagcccc ctttcggtgg
      661 aacggaatat caactcagcc cgctgattac cgatcagcgg gtgcagaaac tgttacggga
      721 attggggtag atgaggcatc agggatctgg gggctgcgcc gtctgatcgt gaccgtgcga
      781 atgtcggagt gcgaggcggg ggcgctgctg gcggtataaa aggctgtatc ccgaacaatc
      841 tgtctggcca gttcgttcag gcaggtttcc gtgacatgtc ccttgagttc ctcgatcatg
      901 atcggggtgg caccgaagag ctgatagcgg gccgtgccgg acgactgacc ttcgtagcgt
      961 ttgacctgcc cgtcccagcg ttccaattcg agtgagacgt tggccgcgta tccatattcc
     1021 agcggaagga agggagtcaa ggtatacatg gatgcgccaa tgacaatgcc tttccaggcg
     1081 gcgccgcccg catgaggatc gatcgtttcg ctcaggagga ttttggctct cacgatttta
     1141 tcgttggatg ccgatacggc gtacgtgccg gaagacaagt gcgagaagag gccgatttca
     1201 gccagcgagc tgaggatccg ttgctgcgtc tcggcggacg tattctgagg agaaccgttt
     1261 ctcgtcgtgc ggattacgtc aatgacaagc ggcacttgtt cgtcgcgagc gatctgttga
     1321 gcgcccgggt cctgcgtttc tgagagaggg gccatttcga tccaacgact gcagccggtg
     1381 gccgctgtca gaatcgatat ggtggcacag cacagaaacg tcaggcggcg aagtgtttta
     1441 gggttcacga tgtgctccta gaagtagaat gagaagcctc cgaacggcag actggagttg
     1501 agccccaggt tgggcatccg ggtgccggcg ttggaaatgt gatgaaaccg gtagccgacg
     1561 ttgaacgaga attgggacgt aaagaaccag gaaaggccgc caccggcggt gaggatgaag
     1621 ttgaagcgcg aagactcttc cggaatacgc ccgccgagat cggtgtaaaa gggaccaccg
     1681 gcaaattcga tgtaaggtct gaggcgaccg gtcccttcga acatgtactt gattttgggt
     1741 gtaaagccga cgccgtgggt gaggaagggc tcttgaaact ggatatacac gatttcggcg
     1801 ccaagcaaaa gttgtccgcg atagaagctc ttgccgatgg gatccgtcaa gaccatcgcc
     1861 cacgatggca tcaaggctgg accgctttgt tttgtggtgt gctcggtcgt caaccggtga
     1921 ggcaacaaat agccggcgct caaactgatt tcctgcgttc cgaccgtcaa atgaggggtg
     1981 acttcccctg cctggcacgg aatcgtcatg gtggacacga gcaacgcgaa ggcgatacgg
     2041 gctgccagcg aaggtggcgt cattcaacat ccggttgaac ctttgtcggc ttgtgtgcac
     2101 gccaacagga ggtacgcaat cgtgacgctt cccaaggtag cagagaatga acgggtgtcc
     2161 agtaagagag gaaattgtgc agagaagatt tcaagcaaga tgtctgaaga tcatcttacg
     2221 gagtacacct cattgtagct gtattgctcg cagacaatgc caggatctcg ttaaggtgag
     2281 attatgaatg tgttaatttc tcgtcc
//
LOCUS       DASTQY010000417         3355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1708738, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000417 DASTQY010000000
VERSION     DASTQY010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708738"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /gene="mltG"
                     /locus_tag="VFR82_13495"
     CDS             complement(<1..747)
                     /gene="mltG"
                     /locus_tag="VFR82_13495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU4502662.1"
                     /translation="MKLRSIFLTLLVGGAVLGIAGYQIIRWAEGPAVPARLNPPNQII
                     RIPDRSSFQQVAALLERERLIKSRAAFVWIGRLQSADRKIQAGEYELNPSMLPVEILA
                     RLLNGQVVLHPVLIPEGLTMVQIADIFGQQGLVDRQELLRLMKDPTFIASLGIQADTL
                     EGYLYPDTYKFPKNVRAKDVLSTMVEHLRQVYTAELHARAQELKMSQHEVLTLASVIE
                     KESASNGEREEISAVFHNRLKKHIPLQSDPT"
     gene            complement(671..1216)
                     /gene="ruvX"
                     /locus_tag="VFR82_13500"
     CDS             complement(671..1216)
                     /gene="ruvX"
                     /locus_tag="VFR82_13500"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU4502663.1"
                     /translation="MAGPRILALDYGTKRIGVALSDELGWTAQPLETFERRTLDRDVA
                     HIVSLVNTHEVGQVVLGFPLQLDGREGPAILAMRDFASRLEQGLPVPLVLWDERLTTK
                     AAEDLLIAADVSRKKRKGTVDRVAAAILLQSYLASRESAEHRVVDSDPQTIDKTDEAS
                     EHFSHSAGRRRRSRHSRVSDH"
     gene            complement(1221..>3355)
                     /gene="alaS"
                     /locus_tag="VFR82_13505"
     CDS             complement(1221..>3355)
                     /gene="alaS"
                     /locus_tag="VFR82_13505"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU4502664.1"
                     /translation="PCGPCSEIHFDQGPAVPGDDRPNGEGDRVIEIWNLVFMQFNRDA
                     AGMLHPLPKPSIDTGMGLERLAAVAQGVHSNYDSDLFLPVLSAIAARAGREYGKQEQT
                     DRSMRVVADHLRAITFLMTDGILPSNEGRGYVLRRILRRAARHGRLLGIVEPFLHELT
                     GTVVQQMGDAYPEVRNAAATVAEATKGEEERFIATLDQGLPILSELIAKTRSGGGTIL
                     PGADVFKLYDTYGFPMDLITEASREQGMTVDEAGFDAAIEEQRTRARKTSGFEQEIAR
                     PAVADIAKRVGATTFVGYDQLESDSIVQAILKGDRLVKEAHEGDEVEVVLDVTPFYAE
                     GGGQVGDQGVLVGAEGRLDIKETTRPVATVILHKGTVSTGRIREGEQLHLSVNRTTRQ
                     DAERNHTATHLVHAALRDLLGPHVKQFGSLVAPNRLRFDFAHFRPLSSRDIDEIESVV
                     NGEIRRNETVRSEVMSIQDAVAKGALAFFGDKYGDEVRVVTVESFSKELCGGTHCRHT
                     GEIGLFRIVSETGVAAGVRRIEAQTGSGAMTMMKKLETEVRELSELLKVGPSELVAKT
                     RKVMAQLKDKERELEELKLKMASGSGVAATARTIAGVPVHVQRTDGLDMNGMRALADQ
                     LRDKLKTGVVALGAAMEDGKVSLLVVVTKDLTGKIKAGDLIKVMAAEVGGTGGGRPEM
                     AQAGGKDSSKLDASLEKVFGLVEHALQR"
BASE COUNT          643 a         1067 c          915 g          730 t
ORIGIN      
        1 ggtcggatcg ctctgcaacg gaatgtgctt cttcaatcgg ttatggaaca ccgccgaaat
       61 ctcttcccgt tctccattag atgctgattc cttttcgatc acggatgcca gcgtcagcac
      121 ctcatgctgg ctcattttca actcctgcgc ccgcgcgtga agctcggccg tatagacctg
      181 ccgcaggtgc tcgaccatgg tcgagaggac gtctttcgct ctgacgttct tgggaaattt
      241 ataggtgtcg ggatacaggt acccttccaa tgtgtcagcc tggattccga gcgaagcgat
      301 aaacgttgga tccttcatga gccgcaacag ctcttgacga tcgacaagtc cctgctgtcc
      361 gaagatgtcg gcaatctgca ccattgtcaa gccttcggga atgaggaccg gatgaagcac
      421 cacttgccca ttgagcagcc tcgccagaat ctccacaggc agcatgctgg ggttcaactc
      481 gtattccccg gcttgaatct ttcgatctgc cgactggagt cgcccgatcc acacgaacgc
      541 ggcccggctt ttgatcagcc gttcacgttc gagcagcgcc gcgacctgct gaaagctcga
      601 ccggtcggga atccggatga tttgattggg aggattgagg cgggcgggaa cagcggggcc
      661 ttccgcccat ctaatgatct gatacccggc tatgccgaga acggcgccgc cgaccagcag
      721 agtgagaaaa atgctccgaa gcttcatcag tcttgtctat cgtctggggg tcagaatcaa
      781 cgacccgatg ctcagccgat tcgcggctcg ccagatagct ttgaagcaag atggcggcgg
      841 ccacgcgatc gacggttccc tttcgttttt tgcggctgac gtcagcggcg atgagcagat
      901 cttctgccgc tttggtcgtc aaccgctcat cccataggac aagcgggacc ggcagtccct
      961 gctccaacct ggaggcaaaa tcccgcatgg ccaggatggc cgggccttct cgcccgtcca
     1021 actgcagcgg aaatccgaga acgacctgcc ccacctcatg ggtgttgacg agggagacaa
     1081 tgtgcgccac gtcacgatcc agcgtgcgtc gctcgaaggt ttccagcggc tgtgcagtcc
     1141 agcccagttc atcgcttagc gcaacgccga ttcgcttggt cccataatcc aacgccagta
     1201 tccgtggtcc agccatgatt ctaccgctga agcgcgtgtt cgaccaagcc aaagaccttt
     1261 tccaacgagg catcgagctt tgaagagtct ttcccgccgg cttgtgccat ttccggacgt
     1321 ccccctcccg taccgccgac ctctgccgcc atcactttga tgaggtcgcc ggctttgatt
     1381 tttcctgtca gatctttcgt cacgacgacg agcaaggaaa ccttgccgtc ctccattgcc
     1441 gctcccaacg cgacgacgcc ggttttaagc ttgtcccgca gttggtcggc cagcgcacgc
     1501 atgccgttca tgtcgaggcc atcggttcgt tgcacatgga ccggcacacc ggcaatggtt
     1561 cttgccgtcg cagctacgcc ggagccgctg gccattttga gcttcaactc ttccaattcc
     1621 cgctctttgt ccttgagttg cgccatgacc ttccgtgttt tggccaccaa ctcggaaggc
     1681 cccaccttta agagttcaga aagttccctg acttccgttt cgagcttttt catcatggtc
     1741 atcgcgccgc tgcctgtctg agcttcgatc cgccggacgc cggcggccac gccggtctct
     1801 gagacgatcc tgaaaagccc gatttcgcct gtgtgccggc agtgcgtacc gccgcacagc
     1861 tccttactga acgattcaac ggtgacaacg cgcacctcgt cgccgtattt atcgccgaag
     1921 aaggccaatg cccccttcgc caccgcatct tgaatgctca tgacctcgga ccgaacggtt
     1981 tcgttcctgc ggatttctcc attgaccacc gattcaatct cgtcaatgtc acgagaagac
     2041 agcggccgga aatgggcaaa gtcgaaccga agccggttgg gagccaccag cgatccgaac
     2101 tgtttgacgt gtggaccaag caaatcacgc aaggcggcat ggaccaaatg ggttgcggta
     2161 tggtttctct ccgcatcctg tcgggtcgtc ctgttcacgg agaggtgcag ctgttcccct
     2221 tctcgaatgc gcccggtgct gacggttccc ttatgcagga tgacagtcgc aacagggcgg
     2281 gtcgtttcct tgatatccag acgaccttcc gcccccacaa gcacgccctg gtccccgacc
     2341 tgcccgccgc cttccgcata aaagggcgtc acgtccaaca ccacctcaac ctcatccccc
     2401 tcgtgcgctt ccttgacgag gcgatcgcct ttgagaatcg cttgcacgat gctgtcggac
     2461 tccagctggt cgtagcccac gaacgtcgtg gcaccgacgc gtttggctat gtcggcgacg
     2521 gccggcctgg ctatttcctg ctcgaatccg ctggttttgc gggcacgcgt ccgctgttct
     2581 tcaatcgcgg catcgaaccc cgcctcatcc acggtcatgc cttgctccct ggatgcctcg
     2641 gtaatgagat ccatcgggaa tccataggta tcgtacagct tgaacacgtc cgccccaggc
     2701 aggatggtgc cgccgcccga gcgggtcttc gcaatcagct cgctcaaaat cggcaggccc
     2761 tgatcgagtg tggcgataaa gcgttcttct tcgcccttcg tcgcttcggc aacggtcgca
     2821 gcggcattgc gcacttcggg ataggcgtcc cccatctgct ggacgaccgt cccggtgagt
     2881 tcgtggagaa agggttccac gatgccgagc agcctgccat ggcgggcggc gcggcgcaaa
     2941 atacgacgca gcacatagcc ccggccttcg ttggatggaa gaatgccgtc cgtcatcaga
     3001 aaggtgatgg cacgcagatg gtccgccacg acgcgcatgg agcgatcggt ctgctcctgc
     3061 ttgccgtatt cccgtccggc ccgagcggcg atggcagaca gcacaggcaa gaagagatcg
     3121 ctgtcatagt tgctgtgaac accttgagcc accgctgcaa gccgttctaa tcccatcccc
     3181 gtgtcaatgc tgggcttggg caaaggatgg agcattccag ctgcatctcg attgaactgc
     3241 atgaatacga gattccaaat ctcgatcacc cggtcccctt cgccgttcgg ccgatcgtcg
     3301 ccgggcacag ccggaccttg atcgaaatga atctcagagc aaggcccgca ggggc
//
LOCUS       DASTQY010000418        12945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_950815, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000418 DASTQY010000000
VERSION     DASTQY010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 12945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12945
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950815"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VFR82_13510"
     CDS             <1..655
                     /locus_tag="VFR82_13510"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4502665.1"
                     /translation="IMSAWRDVLEGLRYVLKGRRIIIFLTITASLYTFATSAFTTLFP
                     VFARKLLDLGPVEVGYLWSMLGIGLLTVSLALTVISAWSVKRRIKVIAVASAVSGLAL
                     LALVMVNHPTEAAGLLIVLGGGLGVLTPVAWGVLQEVAPAHMLGRVLAFYTLGAMAAA
                     MGGITLFGWVTGRFGVPAAVVAIGLVMGCTGLSAWGFLDNMSERPPKREPPLVRDQA"
     gene            943..2271
                     /locus_tag="VFR82_13515"
     CDS             943..2271
                     /locus_tag="VFR82_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4502666.1"
                     /translation="MTTDAVERQQDVTAPTSRMPGWQLLKERDFSLLFWGQMTSQIGD
                     SLNRVALLWFVYQLTGSAMKMVMIGLLQTIPPLVLSPLIGVYLDRANKKTVMVWVDII
                     RSALVLLIPLLYAFDQLTLERLYVAVFLLAIVSTIFGPALSASVPQLVRRDQLTAANA
                     LLQTTTNTGLLIGPLISGVGIALIGSQNVLYVDAVTFFVSALCLIGIRMRPLDRNYLE
                     KGRGTWTQELLAGIRFVYLEQTVLVLMVTAALYSLAASAFAFMLPVFAEQNLSAGALE
                     VGALWSALGAGMLATSTWLASLTQGDLHGRFTLIFRSMAVGGLAMCGLSMLTAPLIAG
                     ALILIIGGSTALFMPIVWGVLQEVTPAPLLGRVFTTFSTGSMASAMAGMAGFGWAADT
                     MGPHVSLLGIGLVLLCTACLAFLSCRRFPSRPRPTPLLSPLDASDQKMAA"
     gene            complement(2346..2978)
                     /locus_tag="VFR82_13520"
     CDS             complement(2346..2978)
                     /locus_tag="VFR82_13520"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEU4502667.1"
                     /translation="MLPDQPYKTKSFNLHGLQGISDKTIDTHLSLYEGYVKATNALQG
                     RIAQFLQDGNVDQEEFPAYSELTRRLGFEFNGMVLHEYYFENLTPQASIKPDNTSAFL
                     RRVEMSFGTYDRWKTDFVSVGKMRGVGWAICFQDPSSGRLSNHWITLHEHGNVAGFQP
                     VLVMDVWEHAFLLDYSPSQRGEYINAFFANVNWRVLDRRIGSDAPHLRAA"
     gene            complement(2997..3548)
                     /locus_tag="VFR82_13525"
     CDS             complement(2997..3548)
                     /locus_tag="VFR82_13525"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEU4502668.1"
                     /translation="MRRFKLFVVVSALVGLLISPLMLVVWAENEPIIQDGSVVLMEFT
                     ITVPESRLVIPKNVSQFTPGHHEMLPNLEKALAGMKKGDEKRVDLSSDDAFGPYDETK
                     KGVISADSLPPDTKPGTILATEEGVPFVVTELSGPVASVDFNHPLAGKHLIIDVKILN
                     VESPSGQEGMRMGSQEENKDISI"
     gene            complement(3621..4193)
                     /locus_tag="VFR82_13530"
     CDS             complement(3621..4193)
                     /locus_tag="VFR82_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502669.1"
                     /translation="MLTVGREMLVVLTIGCVAITAALPVSAAETPSEAQLERLMQMAS
                     QTPDRPDHDYVSNSLQALLQQSQDLKLSREQTDKIRTITDQYERTRRDRETAYKRSEM
                     EALKLIHDNRSSLFSIETAVQKADQEHSKLRMAGIKALREARDVLGPEQYGNWRQSHA
                     ASQLAQGSPHQERGEQYDSDSSGSGRMAPH"
     gene            complement(4370..4849)
                     /locus_tag="VFR82_13535"
     CDS             complement(4370..4849)
                     /locus_tag="VFR82_13535"
                     /inference="COORDINATES: protein motif:HMM:NF039411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HEU4502670.1"
                     /translation="MDPKDNKREIDADQVIRQYEETAYTEEPEEAQTEIPWYEQAKQD
                     TSIPAEAVLTGGDIDAAWDQASVGEETVGGSTPTPDQDIVDEIGRAVGINYEDSEPLD
                     PEAKIERRDENRWELHPASSEDFAERTHSTDIASATSEAQPPSAQSQEPKRNKRKAA"
     gene            complement(5034..5222)
                     /locus_tag="VFR82_13540"
     CDS             complement(5034..5222)
                     /locus_tag="VFR82_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502671.1"
                     /translation="MKPRKRVTANKRRKVQTSEFEREEEYRAVIEPDPLLKEVGEANA
                     RIANPAERERVIPSRPCK"
     gene            complement(5325..6188)
                     /locus_tag="VFR82_13545"
     CDS             complement(5325..6188)
                     /locus_tag="VFR82_13545"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4502672.1"
                     /translation="MKTDTRLILPAKERIAPAAEGTIQFIGNATVLVRYGGITILTDP
                     TFIHRHEQVSIGYGMHSTRLTDPAMEIGDLPPLDLIVLSHFHGDHFDQVAERELDKSI
                     PIITNREAAKELQQRGFRYSKPVDTWSSISVEKGDMRMRVTSMPGRHGPPLSELVMPE
                     VMGSMVEFHPIGGSLFRLYITGDTLIFDDLKEIPRRYKDIDLALLHLGGTRVVGVLVT
                     MDAKQGVELVQLVRPRQAIPIHYNDYDVFTSSLSDFQHEVEAAGLSDRIHYLSHGDTY
                     TIRPTSKRAAA"
     gene            complement(6286..6813)
                     /locus_tag="VFR82_13550"
     CDS             complement(6286..6813)
                     /locus_tag="VFR82_13550"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEU4502673.1"
                     /translation="MKEVAAAGRAEPLRPDDHVIGPDSAPVTVIAYCDFECPYCGRSF
                     AMLKDLRARLEYQMRFVFRHFPLIDKHPLAQQAAEAAEAAASQGQFWTMHDLLFEHQD
                     ALEIDDLYRYAGVARLDLSRFKEDLDRRVHMSRVMRDVTSGRRSGVTGTPTFFLNEAL
                     VPEDERLEDLVLRAA"
     gene            complement(6837..7355)
                     /locus_tag="VFR82_13555"
     CDS             complement(6837..7355)
                     /locus_tag="VFR82_13555"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEU4502674.1"
                     /translation="MPQVTLTGHPLHPQLIVMPAGLLPFSLVLDALHAKTRDQSYADA
                     AYYTMMGGFVGGLVAGAAGAADYSTIPADSKAKEIGRIHGLMNVGLLALTGVNLWLRR
                     RRRSPGILPILLSLLGTAGLTVSAWYGGHLVYHHGMRVRGTELAGGPEDLKLPGDERM
                     AHMLASTAGDEA"
     gene            complement(7415..8182)
                     /locus_tag="VFR82_13560"
     CDS             complement(7415..8182)
                     /locus_tag="VFR82_13560"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU4502675.1"
                     /translation="MARKGGKRYRYRTNTQDIDWQEERADTSHNIGEMQRLFSAIAGV
                     GLIMDGLRRRSLGGGALAVGGVSLLYRAATGYCPAFGAMGIDMGAQDTNRLGRRKVHS
                     GQATKVRRSIDIHRPAPELYRFWRALDNLPRIMSHLKSVEVISDRLSHWVVNTLPGLP
                     TVEWDAEIINDVENERIGWRSLNESDVDHAGSVEFEPSGEGSTRVTVTLQYEPMAGRL
                     GTAVAKLFGQDPELKIADDLQRFKESMEAGTYSQTNR"
     gene            8499..9089
                     /locus_tag="VFR82_13565"
     CDS             8499..9089
                     /locus_tag="VFR82_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502676.1"
                     /translation="MLLRHYLLFFILPLWMAAGLTDYLLHKRTQIEENAGTKESILHA
                     LQLGEAGIPVMLALLLEINALTFLVMVLAFILHELTSLWDVSYASAHRYVSPLEQHVH
                     SFMEVLPLMALSFVTILYWNQFVALIGLGQEPPRFELRPKSNPLSTGYLVALFSCIML
                     FVVLPYGEELWRCLRASRSRHIRHNTKQIFRPSQAA"
     gene            9108..9683
                     /locus_tag="VFR82_13570"
     CDS             9108..9683
                     /locus_tag="VFR82_13570"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEU4502677.1"
                     /translation="MTRFTGLMTVALAHGLLYGCAFVQQTLPGLTMTDAEMVGVLNSF
                     SEEEVEAAELARRTSTTSDVQAFAGRVLDEHRQLAEHNARLSRQLHITPREPALASEL
                     KANHQQAMHDLEGKQGSDFDRAYVEYEIARHVHAFGLMKTAAESEATLPLQQELIRTG
                     PDLLSHLSAARALERRLVAKEDQASESPQSP"
     gene            complement(9864..10712)
                     /locus_tag="VFR82_13575"
     CDS             complement(9864..10712)
                     /locus_tag="VFR82_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502678.1"
                     /translation="MTQQGGCAMGARCKLIVMSVLFLLQGMHAPAMGESASASAEGQK
                     LGEHKEQVVTEPTQNQLPGNRLVTGRILSIRSNQIEIDIGNVKSLYVPLRPAQVKGQT
                     FEVGQPIIVTLNDHNAVVDYHHPNERSDHQVLRGRLTTPLTVGLDKAVIETKDGTKTL
                     MVTDRAKGKLSAIPVGVDAYFLADESGQLVDAQLASTEAVRESGQNNKARIKGAHEQV
                     NAIFKGQGGEGKLKIAEAGNEREVPFRAPLEKLDRLEPGQDVVLLMDDQGYVLEIATP
                     EVPPAR"
     gene            complement(10928..11542)
                     /locus_tag="VFR82_13580"
     CDS             complement(10928..11542)
                     /locus_tag="VFR82_13580"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU4502679.1"
                     /translation="MEARALGQQGNLAEGREDLPSAIVWLTRAARLPAATPSLDWSMN
                     QRDSTSMKGQPARAGKEHPMDHFLIDIEEATIRNNNFRQVLFTAPHSQLVLMSLKPGE
                     EIGTETHRLDQFIRVEAGEGSARLNGQDYALKDGSALVIPAGTEHNVINDRKDQPLKL
                     YTIYSPPEHKDGVVHRTKQEAEADHDDHFDGKTTALLHESPASR"
     gene            complement(11617..12783)
                     /locus_tag="VFR82_13585"
     CDS             complement(11617..12783)
                     /locus_tag="VFR82_13585"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEU4502680.1"
                     /translation="MKANCWYGKRHVQVVDVPDPKILNQRDAIVKITSTAICGSDLHL
                     YNGFVPTMEQGDIMGHEFMGEVVEVGGEVKKLRVGDRVVVAFPISCGNCFFCKGEMYS
                     LCENSNPNAWMAEQFFGHSPAGIFGYSHLTGGFAGGQAEYVRVPFADVGSVKIENGLS
                     DEQVLFLSDIFPTGYMAAENCNISPGDTVAIWGCGPVGQFAIRSALMLGAERVVAIDR
                     VPERLKMARDGGAHTIDYEEADVYDMLMQFTGGRGPDSCIDAVGLEAHAPTPLYYYDR
                     LKQALMLESDRPIALRQAIMACRNGGTVSIPGVYGGLIDKMPMGAVMNKALTLKTGQT
                     HTQRYLTPLLKRIKNGDIDPSFVVTHRLKLADAPQGFATFNDKQDGCIKIVMTP"
BASE COUNT         2599 a         3822 c         3509 g         3015 t
ORIGIN      
        1 catcatgtct gcctggcgcg acgtccttga agggctccgg tatgtgctga aaggccgccg
       61 gatcatcatc tttctgacga tcactgcctc gctgtacaca ttcgccacga gcgcatttac
      121 cacgctgttc ccggtgttcg cgaggaaact gcttgatctc ggcccggtgg aagtcggcta
      181 cttgtggtcc atgctcggaa taggactgct caccgtttcg ctcgcactca ccgtcatcag
      241 cgcctggagc gtgaagcgcc ggattaaggt gattgccgtg gccagcgccg tgagcggtct
      301 cgcccttctg gccctggtca tggtaaatca tccgactgag gcggctggcc tgttgatcgt
      361 cttgggtggc gggttgggag tcctcacgcc agtcgcttgg ggtgtacttc aggaagtggc
      421 accggcccac atgctcggca gagtcctggc cttctatacg ctcggcgcca tggccgccgc
      481 aatgggaggg attaccctgt tcggatgggt gactggtcga ttcggggtgc ccgccgcggt
      541 cgtcgcgata ggactcgtga tgggctgcac cggcctatcc gcctggggtt ttcttgacaa
      601 tatgtccgag cggcccccca agcgtgaacc accccttgtc cgagatcagg cgtaggccat
      661 ccgcgctagg gagcatccga gtcatgaaac catgcgggtg tatggtattg cttaccaaaa
      721 atgcagggac caagaacgag gactctccct aggtcttgat tgtcggggtt tcgacgttaa
      781 tggtagagtg tgcctggtga gataaaactt taggttcgac ctgtggttaa cgaagcactc
      841 gtttctaaac accggttaat ttttactcga agatccggca gtaaacctgg caaggtgtgt
      901 gcaagactcg atcgtcgagc gggtgtgact taggagcaga gcatgacgac cgatgcagtt
      961 gaacgccaac aagatgtaac ggcgcccact tcgcggatgc ccggatggca gttattgaag
     1021 gagcgggatt tcagtctctt gttctggggc caaatgacgt cccagatcgg cgacagcctc
     1081 aatcgagtcg cgcttttatg gttcgtctat caactgaccg gttccgccat gaagatggtg
     1141 atgatcggct tgctgcagac gatcccacca ctcgtcctca gtcccttgat cggggtatat
     1201 ctcgatcgag caaacaaaaa aacggtcatg gtctgggtgg atatcatccg aagcgccttg
     1261 gtcctcctca tcccacttct ctacgcgttt gatcagttga cgctcgagcg tttgtatgtg
     1321 gccgtgttcc tcctggctat cgtgtcgact atttttggcc ccgctttatc agcctccgtg
     1381 ccacaactcg tgcggcggga ccagctgacc gccgccaatg ccctgctgca gacgacgacc
     1441 aacaccggtc tcctgatcgg cccgctgatt agcggggtgg gaatcgccct tatcggctct
     1501 caaaacgtcc tatatgtcga tgctgtgacg tttttcgtgt ccgccctctg cctcatcggc
     1561 atacgcatgc gtccattgga caggaattac cttgagaagg gacgcggcac ttggacacag
     1621 gaactgctcg ccggaatccg ctttgtatat ctcgagcaaa ccgtgctggt cctgatggtc
     1681 acggcagccc tgtacagctt ggcggccagc gccttcgctt tcatgctgcc cgtattcgcc
     1741 gaacagaatt tgagtgctgg cgcgcttgag gttggagcgt tatggtccgc tcttggcgcc
     1801 ggtatgctgg ccacatcgac gtggttggct tctctgactc aaggcgattt acatgggcgc
     1861 tttacgctca ttttccgatc gatggccgtc ggcggactcg ccatgtgcgg tctcagcatg
     1921 ttgacggccc cgttgattgc gggagccttg attctgatca tcggcgggag caccgctctc
     1981 ttcatgccga tcgtatgggg cgtgctgcag gaggttacgc cagcgcccct cctgggtcgg
     2041 gtctttacca ctttcagtac cgggtctatg gcctctgcca tggcaggcat ggccgggttc
     2101 ggctgggcgg ccgataccat ggggccgcac gtcagcctcc tgggtatcgg actcgtactc
     2161 ctctgcaccg cctgccttgc cttcctttcg tgccgccggt tcccaagccg accacgcccg
     2221 acaccactcc tctccccgtt ggacgcgtca gaccagaaaa tggccgcatg acgcgcggcc
     2281 ggagccgcag ggcattcccc acacactaaa tgaaacgtag cgttccgcct ggaagaggat
     2341 ggctactatg ccgcccgcag gtgaggagcg tccgacccga tgcgccggtc tagcacccgc
     2401 cagttcacgt tggcaaaaaa tgcattgatg tactcaccgc gctgggacgg agaataatcg
     2461 agcagaaagg catgttccca cacgtccatc acgagcaccg gttggaaacc ggccacgttc
     2521 ccgtgttcat gcaaggttat ccaatggttg ctaagccggc cggatgatgg gtcctgaaag
     2581 cagatggccc acccgacgcc gcgcatcttt ccgacactca cgaaatctgt tttccaccgg
     2641 tcgtaagttc cgaatgacat ctcaacccgt cggagaaagg ccgaggtgtt gtccggcttg
     2701 atcgatgcct gtggcgtcaa gttttcaaag taatactcat gcaataccat gccgttgaat
     2761 tcaaacccca gccggcgggt gagctcggag taggctggaa attcttcctg atcgacattg
     2821 ccatcctgta agaactgtgc aatacggcct tgcaatgcat tcgtagcttt gacgtacccc
     2881 tcatacagtg acaagtgcgt gtcgatggtt ttatcggaga taccttgcag cccgtgaagg
     2941 ttgaacgact tggtcttata gggttgatca ggcagcatga ctttcctcct ggtggttcag
     3001 atggaaatgt ccttgttttc ctcttgcgat cccatccgca taccctcctg accggatggc
     3061 gactccacgt tcagaatttt gacatcgata atcagatgct ttccagccag cggatggtta
     3121 aagtcgaccg acgctacagg accggacaac tccgtgacca caaacggcac tccctcttct
     3181 gtcgcgagaa tggttccagg tttggtatcg ggtggaagac tgtcggcgct gatcacgcct
     3241 ttcttggttt catcataagg gccaaatgcg tcgtcggacg acaggtctac acgcttttca
     3301 tccccttttt tcatgccggc gagtgctttc tccagattag gcaacatttc atgatggccg
     3361 ggagtgaatt ggctcacgtt cttcggaatg acgagacgtg attctgggac ggtgatcgta
     3421 aattccatca gtacgaccga gccgtcttga atgatcggct cgttctctgc ccacacgaca
     3481 agcatcaagg gggaaatcag taaacccacg agggccgaga cgaccacgaa cagtttgaat
     3541 cgacgcatga tggcgctcct tcccaacgtt gttgcgctgc gtctccagtg gggggttcac
     3601 cagttggcag gcagactcat ttaatgaggc gccatgcgcc ctgaaccgct tgagtcgcta
     3661 tcatattgct cccctcgctc ctgatgagga cttccctgcg ccaactggct cgccgcatgg
     3721 ctttgccgcc agttgccata ttgttccggt cctaacacat cccttgcctc acgcaaggct
     3781 ttgatacctg ccatgcgcag tttgctatgt tcctgatcgg ccttctggac ggctgtttcg
     3841 atggaaaaca atgagctacg gttgtcatgg atcagcttca atgcctccat ttcgctacgc
     3901 ttatacgccg tttcgcggtc acgccgggtc cgctcatact gatcagtaat cgttctgatt
     3961 ttgtccgtct gctcccgcga cagcttgaga tcctgacttt gctgcagcaa ggcttgcagg
     4021 ctattgctca catagtcgtg atccggccgg tccggcgtct gggaagccat ctgcatcagt
     4081 cgttcgagct gagcttctga gggagtctcc gctgcactga caggcagcgc ggcggtgatc
     4141 gcgacacaac caatcgtcag gactacaagc atctctctcc caaccgtgag catgatgtcc
     4201 tccttcaaag ctatttttct agaagcagca agggatgtgc cgtcatacct gttatgccta
     4261 agccgtccga acttagacgc aaggagccgg acaccagctg gcattctgtc cggcgtcgga
     4321 cagcttgtct cgaattctct gaaatcagtg tgaggaacgg tgctctacct caggcggcct
     4381 ttcgcttatt acgcttgggt tcctgacttt gtgctgacgg cggttgcgct tcggaggttg
     4441 ctgacgctat gtcggtcgaa tgagtacgct cggcaaagtc ttccgaggag gcagggtgaa
     4501 gttcccacct gttctcatca cgacgctcaa ttttagcttc cggatccagc ggctcactgt
     4561 cctcgtagtt gatgcctacc gcccgcccga tttcgtcgac gatgtcctgg tccggcgtcg
     4621 gagtggagcc tccaacggtt tcttcaccca ccgacgcctg atcccaggcg gcatcgatgt
     4681 caccaccggt cagcaccgct tcggccggaa tggaggtatc ctgctttgct tgttcatacc
     4741 agggtatttc tgtttgcgcc tcctcaggct cctccgtgta ggcggtctct tcatattgac
     4801 gaatcacttg gtcggcatcg atttccctct tgttgtcttt ggggtccatc aggctacctc
     4861 ctccagttaa ttacgctctt cggcagccgc gatcacgacc ccatcgcgca ccagacaggc
     4921 ggccggatcg tgaaacactg catgaatgcc caacgtgtac acggctgctc attaccaaga
     4981 tgtggaagaa aaaaggatgc gtgcggcgca cggcagatac ggccgaagcc cacttatttg
     5041 cacggcctgc tcgggatgac gcgttcgcgt tcggcagggt tggcgatgcg tgcattcgcc
     5101 tcccctacct ctttcagaag aggatcgggc tcgatgaccg cccgatactc ctcttcccgt
     5161 tcaaattccg acgtttggac cttcctccgc ttgtttgcag tcactcgctt gcgaggcttc
     5221 atgcgttcct tcctccacgt cggattgggt cagactaaaa cgggaaaacg tccgcagaaa
     5281 ctggcacatt ctctagtcac caccggatgg ctgccctggc ctcatcaggc agccgcccgt
     5341 ttggaagtcg gccgaattgt gtaggtatct ccgtgactca gatagtggat ccgatcactc
     5401 aaccctgcag cctcaacctc gtgttgaaaa tcggataatg atgacgtaaa gacatcgtag
     5461 tcattgtaat gaattggaat cgcctgccgc ggacgcacga gttgcaccaa ttcaactcct
     5521 tgcttcgcgt ccatggtcac aagaacgcca accacccgtg tcccacccaa atgcagcagc
     5581 gcaagatcaa tgtctttgta gcgccgcggt atttctttca ggtcatcgaa gatgagggtg
     5641 tcgccagtga tgtacaggcg aaagagcgat ccgccgatcg ggtgaaattc caccatgctg
     5701 cccatcactt ctggcatgac cagctcggat agcggcggcc catggcggcc cggcattgac
     5761 gtgacgcgca ttcgcatgtc gcctttttcg accgaaatgg aggaccaggt gtcgacgggt
     5821 ttcgagtatc tgaagccccg ttgctgcagc tctttcgcgg cttcacgatt cgtaatgatg
     5881 gggattgact tatcgagctc acgctctgcg acctgatcga agtgatcgcc gtgaaaatga
     5941 gacaatacga tgagatccaa tggaggaaga tcgccgatct ccattgccgg atcggtcaac
     6001 ctggtgctgt gcatcccata cccgatcgac acctgctcgt gacggtgaat gaaggttgga
     6061 tctgtgagaa tcgtgatccc gccataacgg accaggacgg tggcattgcc gataaactga
     6121 atggtgccct ccgccgcagg cgcaatccgc tccttcgccg gcagtatgag acgcgtatcg
     6181 gttttcatgt tgtatatttc cccgacggcg agatggcgaa cgacggattt ctcagatcat
     6241 ttccccattc ttacgtcgga tgctcatatc ttatgctgct caccctcatg cggcccgaag
     6301 cacgaggtct tccaaacgct catcttccgg caccagagcc tcgttaagaa aaaacgtcgg
     6361 tgtgccggtg actccgctcc gccgccccga cgtcacatcg cgcatgaccc gcgacatatg
     6421 cactcgacgg tccaagtcct ctttgaagcg actcagatcc aaccgcgcaa cacctgcata
     6481 gcggtacaga tcatcaattt ccaacgcgtc ttgatgctcg aacaacaaat catgcatcgt
     6541 ccagaactgg ccctggctgg ccgccgcctc ggcggcttcg gcggcttgct gggcgagtgg
     6601 atgcttgtcg atgagaggga aatgccggaa gacgaagcgc atctgatatt ccagccgagc
     6661 gcgcaagtcc ttgagcatcg caaacgagcg cccgcagtag gggcattcaa aatcgcagta
     6721 cgcaatcacc gtgacaggcg cagagtcggg acctatgaca tgatcgtccg gccgcaacgg
     6781 ttctgctcgt ccggcggcgg ctacttcctt catgtggaag ccagggcttg cacccgtcac
     6841 gcctcgtccc cggcggttga agcaagcatg tgggccatgc gctcatcgcc aggaagcttg
     6901 agatcttccg gtccacctgc gagctccgtt ccccgcactc tcatgccgtg gtgatacacg
     6961 aggtggccgc cgtaccaggc ggatacggtc aggccggcgg tgcctagaag ggataagagg
     7021 attggaagaa ttcccggaga ccgccgtctg cgacgcaacc acaaattgac gccggtcagg
     7081 gccagcaggc cgacattcat taaaccatgg atgcggccaa tctctttggc cttgctgtcc
     7141 gctggtatgg tgctgtaatc ggccgcacct gccgctccag cgacgagtcc gccaacgaaa
     7201 ccgcccatca tcgtgtaata ggccgcatct gcataagact gatcacgcgt tttggcgtgg
     7261 agcgcatcca ggaccagact gaacgggagc agaccggccg gcatgacgat aagctgcgga
     7321 tgcagcggat gtcctgtcag cgtcacctgt ggcatggtct tccctcttat ccggttctac
     7381 atcacggctt ggcacgagcg gtagccctga ccgatcaacg attagtctgc gaataggttc
     7441 cggcttccat cgattccttg aaccgctgta agtcgtcggc gatcttgagc tccggatcct
     7501 gaccaaacag cttggccacc gccgttccaa gccgacctgc catcggttca tactgtaacg
     7561 ttacggtcac tcgggtgctt ccttctccgc tcggctcgaa ttccacggaa ccggcgtgat
     7621 caacatcgga ctcgttcaac gaccgccaac cgatccgttc attctcgaca tcattgataa
     7681 tctccgcatc ccattcgacg gtcggtaatc cgggcagagt gttgaccacc caatgagaca
     7741 accggtcgct gataacctca actgatttga gatgactcat aattcgtgga agattatcga
     7801 gtgcgcgcca gaagcgatag agctcgggag ctggccgatg gatgtcgatt gatctcctga
     7861 ccttggtggc ctgtcccgag tgaactttcc gtctgcccag gcggttggtg tcctgcgcgc
     7921 ccatgtcgat ccccatggcg ccgaatgccg ggcagtaccc cgtagccgct cgatacagca
     7981 gactgacgcc cccgaccgct agggcgccgc cgcctaatga tcgccggcgc aaaccgtcca
     8041 tgatcaatcc cacaccggcg atcgccgaaa acaaccgctg catctcgcca atgttgtgag
     8101 aggtatcggc tcgttcttcc tgccaatcaa tgtcctgagt gttcgtacga tagcgatatc
     8161 gtttgccgcc tttccgtgcc atgaaatact ccctcccaaa tcgaacggtt gaccagggca
     8221 aggcaacagc ttgatttgcc ttgccatgga agtgtgttgc aaaacacaga cccttgagaa
     8281 acgcttgacc agagcctcaa aaaccgagca gctctgcaac gaacgacata aagtgtagga
     8341 tcatcatgat ttatttagac agactgtctt gcgctggaca gcttttccag attttccatc
     8401 ccagggtcgt gccattgcat cagcggtagg ccttaggatg ttccccaggc cacaacgagg
     8461 ctccgacgga ctagcatgaa gtcatggtgg atcctgcgat gctgctcaga cactacttgt
     8521 tgttttttat ccttccctta tggatggctg cgggcctcac tgattacctc ttgcacaagc
     8581 gcactcagat cgaagaaaac gcagggacga aagaatcgat cttacatgcc ctgcagctcg
     8641 gcgaggcagg aatccccgtc atgctggccc tcctgctgga gatcaacgca cttacctttc
     8701 tggtcatggt gctcgcattc attctgcatg agttgacctc actgtgggac gtcagttacg
     8761 cgtcggcaca tcgctacgtc agcccattag agcagcatgt gcacagtttt atggaagttc
     8821 tgcccttgat ggcactgtcc tttgtgacga tcctgtattg gaatcagttt gtggccctta
     8881 tcggcctggg ccaggaaccg cccagatttg aattgcgccc caagtccaat cctctttcga
     8941 ccggttatct tgtcgcgctc ttctcatgca tcatgctctt cgtggtactg ccgtacggtg
     9001 aagagctctg gcgctgcctg cgcgcttcgc gatcacggca cattcggcac aacacgaaac
     9061 agatattccg gccgtcacag gcggcctaag ccctaggaga agctcaagtg actcgcttca
     9121 ccggtttgat gacggtggca ctggcgcacg ggctactcta tgggtgtgcc tttgtgcaac
     9181 agaccctccc gggactgaca atgacggacg ctgagatggt cggcgtgtta aacagtttct
     9241 ctgaagaaga ggtcgaggcg gccgagctcg cgcgtcgcac gtcaaccact tccgatgtcc
     9301 aggcgtttgc cggacgagtg ctcgacgaac atcggcaatt ggcagaacac aatgcacgtc
     9361 tctcccgaca attacacatc accccgcgtg agccggcgct ggcatccgaa ctgaaagcca
     9421 accatcaaca ggcgatgcat gatctcgagg ggaagcaggg atcggacttc gaccgcgcct
     9481 acgtggaata tgagattgcc cgacacgtgc atgccttcgg cctgatgaag acggctgccg
     9541 aatccgaagc caccctcccg ctgcaacagg aactcatccg aacgggacct gatcttctca
     9601 gccatttgtc ggcggcgcgg gcattggaac gccgactcgt tgcgaaagaa gatcaggcat
     9661 ctgaatcgcc acagtccccc taggccactc gctgaatccg aaggggagcg cgagagtggc
     9721 tgtgccggca tgcagcccga atcggaaatg ggagtggcaa gctctgtgat gagagcgaat
     9781 caaaggcctt tatgtaagcc agtcggggtg gagtcgtcag gggccgtagc gctcaattat
     9841 caatacagcc cccgaccttt ttgctaccgc gcaggcggga cctccggcgt agcgatctcc
     9901 aggacgtacc cttgatcgtc cattaataag acgacgtcct gcccgggttc cagacggtcc
     9961 agtttttcaa gcggagcacg gaacggcact tccctctcat tgcccgcttc ggcaattttt
    10021 aactttcctt cgccgccctg ccctttgaaa atggcattca cttgctcatg agctccttta
    10081 attctggctt tgttgttctg gcctgattct cggaccgcct cggtgctggc gagttgggcg
    10141 tctactaact gtccgctctc atcagccaaa aagtaggcat cgacacccac cggaatggcc
    10201 gagagctttc ctttcgctcg atcggtcacc atcaacgtct tggttccatc cttggtttca
    10261 atcaccgcct tgtccaatcc cacggtgagc ggggttgtca aacgaccgcg caatacttga
    10321 tggtcggacc gttcattggg atgatgataa tcgaccacgg cattgtggtc gttcagtgtg
    10381 acaataatcg gttgtccgac ctcaaacgtt tgacccttca cctgagctgg ccggagcggc
    10441 acgtaaagtg acttgacgtt cccgatatcg atttcgattt ggttactccg aatcgacaga
    10501 atgcggccgg tgacaaggcg attgccaggt aactggttct gagtcggttc agtgacaacc
    10561 tgctctttat gttcccccag cttttgcccc tccgccgacg cgctcgcgga ttctcccatt
    10621 gcaggtgcat gcatgccctg cagcaggaaa agcacactca tcacgatgag tttgcacctt
    10681 gcacccattg cacacccccc ttgttgtgtc atggattctc ccctcacaat tattccgagc
    10741 aagtgttgga ccaatcagcg gattgtgcga agccgaaaac ggcctgtctc ggggtcaaaa
    10801 atactcgaac cgactagtca gcacccgagt catttagaca ggctgtctga gcactggaca
    10861 ggatgctcga caagtcaacg ttgacaggta tccatcacca atgcgccttc ttcgattccc
    10921 ctgccgctta ccgagacgcc ggcgattcgt gcaatagcgc cgtggtcttt ccatcgaagt
    10981 ggtcatcgtg gtccgcttcc gcctcttgtt tcgtccggtg gacgaccccg tccttgtgct
    11041 ctggcggact gtaaatggtg tacagcttca acggctggtc tttgcggtcg ttaatgacgt
    11101 tatgctcagt cccagccgga atgacaagcg cgctcccgtc tttcagggcg tagtcctggc
    11161 cattgagacg ggcgctgcct tcgcctgcct cgacacggat gaactgatcg aggcggtgtg
    11221 tctccgtgcc gatctcctct ccgggtttca aactcatcaa gaccagctga ctgtgagggg
    11281 cggtgaacaa tacctgacga aagttgttgt ttcgaatcgt tgcctcttcg atatcgatca
    11341 agaaatgatc catcgggtgt tcctttcctg ctcgggctgg ctggcccttc atagaggttg
    11401 agtctcgttg attcattgac cagtcgagtg aaggagtggc tgctggaagc ctggctgcgc
    11461 gggtcagcca gacgatggcc gagggcaaat cctcacgccc ctccgccagg ttgccttgct
    11521 gaccaagtgc ccgggcctcc atcagcacgt gcatcctttg tccaagcggc acgtcggtaa
    11581 tacgggtttc atgtctactc cttcggtgag cgctcgtcag ggagtcatca cgatcttgat
    11641 acaaccgtcc tgtttatcgt tgaacgtcgc gaacccctga ggcgcgtcag ccagtttcaa
    11701 ccggtgcgtc acgacaaagc ttggatcgat gtcgccgttc ttgatccgtt tgagcagcgg
    11761 cgtcagatag cgttgcgtat gcgtttgtcc cgttttgagc gtcaaggcct tgttcatcac
    11821 cgcccccatc ggcattttgt cgatcaatcc tccgtacacg cccggaatcg ataccgtccc
    11881 gccgttacgg caggccatga tggcctgacg cagcgcgatc ggccggtcgg actccagcat
    11941 cagcgcctgc ttgagacggt cgtagtaata caagggagtg ggtgcgtggg cttcaagtcc
    12001 aaccgcatcg atacatgaat caggccctcg cccacctgtg aactgcatca gcatgtcgta
    12061 gacgtcggcc tcctcatagt cgatcgtgtg agcgcctccg tcacgggcca tcttcagccg
    12121 ttcgggcacg cggtcgatcg cgaccacacg ctcagcgccg agcatcaacg cgcttcggat
    12181 cgcaaattgg cccacgggac cgcaccccca aatggccacg gtgtcgcccg gggagatatt
    12241 gcagttttct gcggccatgt atccggtcgg gaaaatatcg gaaaggaaca gtacctgttc
    12301 gtcggacaag ccgttttcaa tcttgacgct gccgacatcg gcgaaaggga cgcgcacata
    12361 ttcagcctgt ccaccggcga atccaccggt gagatgggaa tagccgaaga tgccggccgg
    12421 ggaatggcca aaaaattgct ctgccatcca ggcattcgga ttggaattct cgcacagcga
    12481 atacatttcc cccttgcaga agaagcagtt gccgcacgaa atgggaaatg cgaccaccac
    12541 tcgatcgccc acgcggagct ttttcacttc ccccccgact tcgaccactt ctcccatgaa
    12601 ttcatgcccc atgatgtcgc cttgttccat ggtgggcacg aaaccgttat agaggtgcaa
    12661 atcggacccg cagatcgccg tcgaggtaat tttgacgatg gcatcgcgct gattgagaat
    12721 ttttggatcc ggcacatcaa ccacctgcac atgtcgtttt ccataccaac aattggcttt
    12781 catcgttctc cttcctgcat tcctagtctt gaaggggacg ccgcgtggat tgggtcaccg
    12841 tccactcgat cgaggtgttc ttgatccgcg cacggcccgg tcatatttcc aggaggtgct
    12901 cccctgtcgt ccggccggct ggccttcggt cgtaatgacc tcgcc
//
LOCUS       DASTQY010000419         2387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_810088, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000419 DASTQY010000000
VERSION     DASTQY010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810088"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VFR82_13590"
     CDS             complement(<1..1304)
                     /locus_tag="VFR82_13590"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEU4502681.1"
                     /translation="MHHPSWFLFLTAFVTGAVVMALEILGSRLLAPVFGNSLFVWGAL
                     IGVILAAMSSGYAFGGWISDRLPGGHVLAGLLLFSGAWTFLIAWASQPILFKVAGVIE
                     DPRWGPCLAATVLLAPPAFGLSGVLPAMLRLAVSDLNYLGRHTGRMIALSTVGSLAGT
                     WGTAFFFLSWIGSQALVAWLGVTQTALGFWWLLKGGAAKGVTVLVFAMLIGGLAVGAW
                     SPVQMLKPPVYQEDSPYQQVRIREDDLFRYLVLDRTFHAVMWKAEPVTLFLPYSQLMV
                     SSLALVPEAKRALVLGHGGGSFAKWLARYWPDLDLDVVEFDPVVVRMAEDYFSYRPPS
                     RHHVYVRDARAYLNGTANRYDLIWVDAFARHLVPFHLTTQEFFSDLRKHLTPEGVVVV
                     NLAASGNRGDLGRAKAVVQTMKQSFPVIETYAVKGPWPSKSRA"
     gene            1532..>2387
                     /locus_tag="VFR82_13595"
     CDS             1532..>2387
                     /locus_tag="VFR82_13595"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEU4502682.1"
                     /translation="MQISVIGTGYVGLVTGACFAEFGVNVLCMDNDAKRIARLEKGDV
                     PFYEPGITELVAKGIGQGRLNFTAELTRAVDHGEVIFIAVGTPPKKDGSADLSFVEEV
                     GRGIAGKMASYKVVVTKSTVPVGTGAFLQRVIASTQARKVAFDVVSNPEFLREGSAIE
                     DFMRPNRVVIGSDSERAVALMKDLYRPLYLIETPFVVTDVATAEMIKYASNAFLATKV
                     SFINEIATLCERVGADVQTVAKGMGLDQRIGSKFLHAGPGFGGSCFPKDLAALVQMGE
                     KAGYPMQIA"
BASE COUNT          561 a          695 c          674 g          457 t
ORIGIN      
        1 gcacgtgatt tggacggcca tggtcctttg accgcatagg tctcgatgac ggggaaggat
       61 tgcttcatcg tttgcaccac cgctttggct cttccgagat cgcccctgtt cccggatgcc
      121 gccagattta cgacgacgac accctccggc gtcagatgct tccgcaagtc tgaaaaaaat
      181 tcttgagtcg tcagatgaaa cgggacgagg tgtcgagcga acgcatcgac ccagatcaga
      241 tcgtacctgt tagcggtacc gttcaagtag gctctggcgt cacgaacgta cacatgatgt
      301 cgagagggag gacggtagga aaaatagtct tcggccatcc tgacgaccac gggatcaaat
      361 tcaaccacat ccaggtccaa atcgggccaa taccgggcga gccacttggc aaaggatccg
      421 ccgccatggc ccagaaccaa cgctcgcttt gcctcaggca ccagagccag ggatgacacc
      481 atgagctggc tatacggcag aaagagggtg accggttcag ccttccacat gactgcatga
      541 aatgttcggt cgaggacgag gtagcgaaac agatcatctt ctcgaattct aacctgctga
      601 tagggactgt cttcctggta gaccggcggc ttcagcattt ggacgggaga ccacgcgccg
      661 actgccagcc ctccgatgag catcgcgaag accagcacgg tcaccccttt ggcggccccc
      721 cctttgagga gccaccagaa tccgagtgcc gtctgcgtga cacctaacca cgcgaccaag
      781 gcctggctgc ctatccatga caggaaaaaa aaagccgttc cccaggtgcc cgccaggctt
      841 ccgactgtcg acagggcgat catcctgccg gtatgtcgcc cgagatagtt caaatcggag
      901 acggcgagcc gcagcatcgc cggcaacacg ccgcttaacc caaatgccgg cggtgcgagc
      961 aagacggtgg ccgcaagaca cggtccccac ctggggtctt cgatcacccc cgccaccttg
     1021 aacagaattg gctggctcgc ccatgcgatg agaaacgtcc acgctcccga aaacaaaaga
     1081 agtcccgcga gcacatgccc ccctggcaga cgatcagaaa tccaacctcc gaaagcgtat
     1141 ccgctgctca tcgccgctag aatgactccg atgagagctc cccacacaaa gagcgagttg
     1201 ccgaagacag gagccagcag gcggctaccc agaatttcca acgccatcac cacggcaccc
     1261 gtgacgaacg cggtgagaaa caggaaccaa ctgggatggt gcactgacaa gtgctgggtc
     1321 atgcagaaag aaggggtttc attcaatgct cttcggacgg ccgctcgttg tgcatgtgcc
     1381 gcggggggcg ctcatagtag ccaccgcgtc tggaagctgt caacgaagcg ggcctctacg
     1441 tgtgactgca gccatacatg aatcctcttt gcccctggcg acggtccaga gtatactgcg
     1501 cgggtccatc gcgtagagga ggcaacgacg tatgcagatc agtgtcatcg gaaccgggta
     1561 tgtcggactg gtcacgggcg cctgtttcgc cgaattcggc gtcaacgtgc tttgcatgga
     1621 caacgatgca aaacgcatcg cacgcctcga gaaaggggat gtcccattct atgaacccgg
     1681 cattacagag ctcgttgcga aaggtatcgg ccaaggccgc ctcaatttta cagccgaact
     1741 tacgagagca gttgaccacg gggaagtgat ttttatcgcc gtcggaacac ctcctaagaa
     1801 ggacgggtcg gccgatctct cgttcgttga agaagttgga cgcggaattg cgggcaagat
     1861 ggcctcctac aaagtggtcg tcaccaagtc gacggttccc gtgggcacag gtgcgttctt
     1921 gcaacgggtc atcgccagta cccaggccag aaaggtggcg ttcgatgtcg tatcgaatcc
     1981 ggaattcctg cgcgaaggct cagctattga agactttatg aggccaaacc gcgtggtgat
     2041 cggatcggac agtgagcgtg ccgttgccct gatgaaagat ttatatcgcc cgctctacct
     2101 gattgaaact ccgtttgtcg tcacggatgt ggccacggca gaaatgatta aatatgcctc
     2161 gaacgcattc ctcgcgacca aagtttcctt tatcaacgag atcgcgacac tctgcgaacg
     2221 ggtcggggcg gatgttcaaa ccgtagccaa gggaatgggc ctggaccagc gaatcgggtc
     2281 caagttcctt catgccggtc ccggatttgg cggatcttgc tttccaaagg accttgccgc
     2341 cctcgttcaa atgggcgaga aggccggtta ccctatgcaa atcgccg
//
LOCUS       DASTQY010000420         3490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_299369, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000420 DASTQY010000000
VERSION     DASTQY010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299369"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..>3490
                     /locus_tag="VFR82_13600"
     CDS             199..>3490
                     /locus_tag="VFR82_13600"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4502683.1"
                     /translation="MPLTGFHPLIAGWFQSSVGVPTDVQAQAWPAIRSGQDALIAAPT
                     GSGKTFAAFLSSIDQLFVQALARELDDRTHVLYVSPLKALSNDIQKNLQQPLADIGQA
                     ALQAGLLMPELRVLVRTGDTPMSDRQQMLKRPPHILVTTPESLFILLTAEKSRRLLQT
                     VRTVIVDEIHALAPNKRGAHLALSLERLEALTLTKPQRIGLSATQRPIDLVARFLTGD
                     RPRPTVIDVGHKRQMDVAVEVPKDELSAVVTNAIWSDIYDRVAALVREHRSTLVFVNT
                     RRLAERVAHHLEERLQDLGPQSVAAHHGSLSREIRLSAEQRLKSGKTRVVIATASLEL
                     GIDVGTVDLVCQIGSPRAIATCLQRIGRAGHWIKAIPKGRLFATTRDELLECAALIRA
                     MRAGTLDRISVPPAPMDILAQHIVAAAATQSWTEAELFALIRRAYPYQELTRNEFDIV
                     VRMLADGIATNRARGHAYLYHDRINHVFKGRRGARLAAITSGGAIPDTANYAVVAEPE
                     GTVVGSVDEDFAVESLAGDIILLGNTSWRIKGVEMGRVRVEDAHGAPPSIPFWRGEAP
                     SRTAELSAEVADLRRDIAERALDTQHSAVQWLKQECALDQRGAQQAIEYVVGSKAILG
                     AVPTQATIVAERFFDESGGMQLVIHAPFGGRITKAWGLALRKRFCVTFDFELQAAATD
                     NGLVISLGEKHSFPLESVFNYLRSESVREVLLQAVLQAPMFATRWRWNASRSLALLRF
                     ANGKKVPPQIQRMRAEDLLAAVFPDAIACQDNMTGERERRIPDHPLVQETLRDCLTEA
                     MDVDGLTDVLDGIEAGAIRCVAIDTPSPSPFSHEILNANPYAFLDDAPLEERRARAVA
                     LRRTLPGEMAGDVGSLDSAAIDEVVRESWPVVRDADDLHDALLTLVWVPAPEVSGWQQ
                     FLPELVQSGRATELAIDVGETMTVGQRAGKAIGWVATEHLANVQRALAGDDEESTVDA
                     IVHGWMESIGPITTEELARRLWLQTDRVNASMMRLEAQGQVLRGRFRPRSGDDGGLSE
                     WCHRRLLARIHRLTIGTLRKQVEPVATADFMRFLFQWQHVTSGSRQHGEAGLLEVIRQ
                     LSGFEAAAS"
BASE COUNT          675 a         1012 c         1106 g          697 t
ORIGIN      
        1 tctaccgagc caatcaggac aatccagcga tcatcgaact gtatcgtgtt caattctcgg
       61 ccccgggagt gagcaccaaa ctcaacagcc agctggttcc aggaaagaat gtcagagatt
      121 ttgctgtgaa ctgatcggta gatcagatct cgcgggcggc tcttgactcg tcgctgacgg
      181 cgccatagca tcaatggaat gccgctgaca ggcttccatc ccctcattgc cggctggttt
      241 caatcctccg tcggcgttcc cacggacgtt caagctcagg cctggcccgc gattcggtcg
      301 gggcaggacg ccctgattgc tgcgccgacc ggctcgggga agaccttcgc cgcatttctt
      361 tccagcatcg accagctctt cgtgcaagca ctggcccggg agcttgatga tcgcacgcac
      421 gtgctgtacg tctcgccgct caaggcgctg agcaacgaca tccaaaaaaa cctccagcag
      481 ccgctcgccg acatcggaca agccgcactg caagccggac ttctcatgcc cgagctgcgt
      541 gtgctcgtcc ggacgggcga taccccgatg tcggaccgtc agcagatgct caaacggccg
      601 ccgcatatcc tcgtcactac tcccgaatcc ctattcattc tgctgacggc cgagaaaagc
      661 cgccgcctcc tgcagaccgt tcgcacggtc atcgtggatg aaattcatgc gttggctccc
      721 aacaagcgcg gcgcccacct cgcactgtca ttggaacgac tggaagcctt gaccctgaca
      781 aagcctcagc gaatcgggtt gtccgccacg cagcgcccta tcgacttagt cgcgcgcttc
      841 ctgaccgggg accgtccccg tcccaccgtc atcgacgtcg gccataagcg gcaaatggat
      901 gtcgcggtcg aagtacccaa agacgaattg agcgccgtcg tcaccaatgc gatctggtcg
      961 gatatttatg atcgggtggc cgctctcgtg cgagagcatc gcagcacgct ggtgttcgtc
     1021 aatacgcgcc ggttggccga acgcgtcgct catcacctcg aggaacggct tcaggatttg
     1081 ggaccacaga gtgtcgcggc gcaccatgga agtctttcgc gggagattcg tttgtcggca
     1141 gagcagcgcc tgaagagcgg gaagactcgc gtggtgatcg cgaccgcctc gctggaactc
     1201 ggcatcgatg tcggcaccgt cgatttggtg tgccaaattg gatcgcctcg cgcgatcgcc
     1261 acttgcttgc agcggattgg ccgagcgggg cactggatca aggcgattcc caagggacgg
     1321 ctcttcgcca cgacgcggga tgaattgctt gaatgcgctg cgctgattcg ggcgatgagg
     1381 gcgggcacgc tggatcgcat ctccgtgccg ccggctccga tggatatact tgcgcagcac
     1441 atcgtggccg cagcggccac ccagtcgtgg accgaggcgg agctcttcgc gctcattcgg
     1501 cgtgcgtatc cctatcagga gttaacacga aacgaatttg atatcgtggt acgcatgttg
     1561 gcggatggca tcgccaccaa ccgggcgcgt ggacatgcct atctctacca cgatcgaatc
     1621 aaccacgtat tcaaaggtcg ccgtggagcg cgattagcag cgattacctc aggcggggcc
     1681 attccggata cggccaacta cgcggtcgtg gccgagccgg aagggacggt ggtgggctcc
     1741 gtggacgagg attttgccgt ggagagcctc gctggcgata ttatcctgct cggcaatacg
     1801 tcttggcgga tcaaaggcgt cgaaatgggc agggttcgtg tcgaggatgc ccacggcgca
     1861 ccgccgagta tccctttttg gcggggggag gcgccgtcga gaaccgccga gctctccgca
     1921 gaggtggccg atctccgcag agacatcgct gaacgagcac tcgataccca acactcagca
     1981 gtgcagtggc tcaaacagga atgcgccctg gaccaacgag gcgcccaaca ggctatcgag
     2041 tatgtggtcg gcagtaaagc gatccttggg gcggttccga cgcaggccac gatcgtggcc
     2101 gagcggtttt tcgacgagag cggcggcatg cagctcgtca ttcatgcgcc gttcggcgga
     2161 cgcattacca aggcctgggg gttggccttg cgcaagcggt tctgcgtcac gttcgatttc
     2221 gagctgcagg cggcggccac cgacaatgga ctcgtgattt cactcggtga aaaacacagt
     2281 ttccccttgg agtccgtgtt taattatctc cgttcagaga gcgtgagaga ggtgttgcta
     2341 caagcagtcc tccaggcgcc gatgttcgcg acgcgttggc gttggaatgc ctcgcggtcg
     2401 ttggcgctcc taagatttgc caacggcaag aaggtaccgc cgcagattca acggatgaga
     2461 gccgaggatc tgctcgcggc ggtttttcct gatgcgatcg cctgtcagga caacatgacg
     2521 ggggagcggg agcgccggat ccccgatcat ccactggtgc aagagacatt gcgtgattgt
     2581 ctgaccgaag cgatggacgt ggatggactg acagacgtgt tggatggaat cgaagcgggc
     2641 gctattcgtt gtgtcgccat cgatacgcct tcgccgtctc cgttctcgca cgagattttg
     2701 aatgctaatc cctacgcgtt tctcgacgac gcgccgctcg aggaacggcg tgcgcgtgca
     2761 gtggcactgc gccgcacctt gcccggtgaa atggctggcg acgtcggctc cctcgattcg
     2821 gccgcgatag acgaggtggt gcgcgaatcc tggccagtcg tgcgcgatgc cgacgacctt
     2881 cacgatgcgc tgctgacgct ggtgtgggtc cccgcgccgg aggtgagcgg ctggcaacaa
     2941 ttcctgccgg agctggttca gtcgggacgg gcgacggagt tggcgatcga tgtcggtgaa
     3001 acaatgacgg tcggtcagag ggccggcaag gccatcggat gggtcgcgac tgaacatctg
     3061 gccaatgttc agagagcgct ggcaggtgat gatgaggaga gcacagttga tgccattgtt
     3121 cacggttgga tggaaagcat cgggcccatc acgacggagg aattggccag gcggttatgg
     3181 cttcaaacgg atcgagtgaa tgcgtcgatg atgcggctcg aagctcaagg ccaggtgttg
     3241 cgagggcggt tccgaccgcg tagcggcgac gatggcggct tgtctgagtg gtgtcatcga
     3301 cggcttctgg ctcgcattca tcgcctgacc atcggcacgt tgcgcaagca ggttgagcct
     3361 gtggcgacgg ctgacttcat gcggtttctc ttccaatggc aacatgtgac gtctggctca
     3421 cggcaacacg gggaagccgg cctacttgaa gtgattagac agctgagtgg atttgaagct
     3481 gccgcgtcga
//
LOCUS       DASTQY010000421         5589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1341871, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000421 DASTQY010000000
VERSION     DASTQY010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341871"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VFR82_13605"
     CDS             <1..1072
                     /locus_tag="VFR82_13605"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4502684.1"
                     /translation="RGKGNAEVVVVTGASAGVGRAIAREFARHGARVVLLARSQDGLD
                     GAARDVERLGGRALALTTDVSDDQQVEEAADRVEGDLGPIDIWVNNAMVSVLSPALQM
                     TAEEFRRVTDVTYLGYVYGTLSALRRMVPRDRGVVVQIGSALGYRAIPLQSAYCAAKH
                     AIQGFTESLRSELIHDGSHVRLTTVQLPAVNTPQFSWIRTRMPNHPQPVPPIYQPEVI
                     ARAVYWTAHHRRREFAVGYPTVQAILGDKFIPGLLDHYLAWVGYESQQTRQPVPPNRA
                     DNLYKPLPGDFGARGEFSDRAHNYSIHSWINRYRGWLALGGLGLATWWWQGRQALREP
                     GPRQKFSSSRTSKRRAFQRFAA"
     gene            1069..2373
                     /locus_tag="VFR82_13610"
     CDS             1069..2373
                     /locus_tag="VFR82_13610"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEU4502685.1"
                     /translation="MTDLIAARTQMAMSLGFHIVFAVLGIAMPALIAAAEWRWLSKGD
                     RNGLSLAKRWSKGTAILFAVGAVSGTVLSFELGLLWPTFMERAGPSIGPLFALEGFAF
                     FTEAIFLGIYLYGWSRINPRVHFLSGLIVVASGIASAVFVVTANAWMNAPSGFVLQEG
                     RLSQINPLAPLWHPVAWHEAGHMLLGAFAATGFLVAAIHAFWLLKEPDSAFHRLALSI
                     ALAIGGPPALLQPLSGDLIAKSVAVHQPAKLASMEALFATKTGAEFVLGGLPDAATRT
                     VPYGLLIPSGLSLLLYGDANAQVIGLDHFPPEDWPPVAVVHVAFQIMVGCGLLMATIA
                     AWSGWRWWRAGRLKLDRPWLLTLIASGPLGFVAIEAGWVVTEVGRQPWIIDHVLRTAD
                     AVTPMPGLWIPMVVFTLLYVVLAGVVLWAIWRHIAATYLSPT"
     gene            2499..3527
                     /locus_tag="VFR82_13615"
     CDS             2499..3527
                     /locus_tag="VFR82_13615"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEU4502686.1"
                     /translation="MGTLEVLLGASLVGALTCYLLTGGADFGAGIWTLFACGRNGQRQ
                     RALIDQAIGPIWEANHVWLIVAVTILFTAFPPAFSVIATSLHIPLSLMLVGIVLRGTA
                     FAVRTHDITSRDGSSSVSPLWRHLFAVSSIITPALLGISVGAVASGRLPVASSPSSSF
                     VERFIAPWLAAFPLSVGLLTTTLVAYLAAVYLLVESQDPELRNAFRRRAQVACVALVL
                     TGAYALVQARQGAPEIYAGLRHTPAGLLAIVVTFGLHMAALVTLWKARDRAARIFAVA
                     GAVIMLWGWALSQYPYLVEPSITIADAAPTETLRLLLVSLILGSIILFPLLYYLYRIF
                     KGRVVFDI"
     gene            3569..3820
                     /locus_tag="VFR82_13620"
     CDS             3569..3820
                     /locus_tag="VFR82_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502687.1"
                     /translation="MSTTLVRFLTGAIVLAAVCWSIFSWQHPVAQGQEAKRYAYKVVD
                     VPPDNTAMQNILNEYGAAGWELIVIGMGDMTSPRLVFKK"
     gene            4455..4931
                     /locus_tag="VFR82_13625"
     CDS             4455..4931
                     /locus_tag="VFR82_13625"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4502688.1"
                     /translation="MLRIKDVMTPQAEVISPDATAEDAAAIMKTLDIGVLPVSDEQGL
                     VGILTDRDLVVRVMAATRDPKAMLVGEAMTPSVVYCFEDDDIEQAATVMAGQQIRRLP
                     VLDKNRKLVGIVSMGDIAVHGREPQLSGKVLEDVSQPATPNRSDQAKRESGFGQAA"
     gene            5218..5511
                     /locus_tag="VFR82_13630"
     CDS             5218..5511
                     /locus_tag="VFR82_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502689.1"
                     /translation="MKQLTYWESVSIGWRILWQGIGGFVLALFLGSLAVVGLLPELTR
                     TGPSYGALVVPLVSASVLSLFVMLPLVVRGLINASFGTFRLVVMRKEPCQSTV"
BASE COUNT         1015 a         1654 c         1628 g         1292 t
ORIGIN      
        1 tcgcggaaaa gggaatgctg aagtcgttgt cgtcaccggg gcgtcggccg gcgtcggacg
       61 agccattgcc cgcgagtttg ctcgtcacgg agccagggtc gtcctgttgg cgcgtagcca
      121 ggacggattg gatggcgcgg cacgagatgt cgagcgtctg ggcggccgcg ctttagccct
      181 cacgaccgat gtctcggacg accagcaagt cgaagaagcg gccgaccgcg ttgaaggcga
      241 cttgggcccg atcgacatct gggtcaataa tgcgatggtg tcggttctgt ctccggccct
      301 ccagatgacg gcggaggagt ttcgccgcgt cacggacgtg acctaccttg gctacgtcta
      361 cgggacgtta tcggcgctgc ggcgcatggt gccgcgcgat cgtggcgtcg tcgtgcagat
      421 cggctctgca ctgggctatc gcgctattcc actgcagtcc gcttattgtg cggcgaaaca
      481 tgcgatacaa ggtttcacgg agtcgcttcg gtcggaactc attcatgacg ggagccatgt
      541 gcggttgacc acggtgcagc tgcctgccgt caataccccg caattttcat ggattcgcac
      601 gcggatgccg aaccatcccc aaccagtccc cccgatttac caacctgaag tcatcgcacg
      661 ggctgtctat tggacggcgc accaccgtag gcgtgaattt gccgttggct atcccaccgt
      721 ccaagccatc ctcggcgata aattcattcc cgggctgctc gatcattacc tggcgtgggt
      781 aggctacgag tcgcaacaaa cgaggcaacc tgtgcctccc aatcgagctg acaacctcta
      841 taagcctctt cccggtgact tcggtgctcg aggtgagttc agcgatcgtg ctcacaatta
      901 tagtatccac tcgtggatca atcgatatcg aggctggctg gcccttgggg gacttggcct
      961 ggcaacgtgg tggtggcaag gcaggcaggc tctaagggaa cccggaccga ggcaaaagtt
     1021 ttcgtcgtct cgcacatcca agcgtcgcgc ttttcagcga ttcgccgcat gacggatctc
     1081 attgccgcac gcacgcagat ggcgatgtcg ttgggctttc acatcgtctt cgcggtgctc
     1141 ggtatcgcca tgcccgccct gatcgccgcg gcggaatggc ggtggttgag caagggagac
     1201 aggaacggtt tatccttggc caagcgctgg tcgaagggaa ctgctatcct tttcgccgtc
     1261 ggtgcggtgt ccgggacagt gctttcattc gaactcggtc tcctgtggcc gacgttcatg
     1321 gaacgggcag gaccgtccat cggtccgttg ttcgccctcg aagggttcgc attctttacc
     1381 gaagccattt ttctcggcat ttatctgtac ggctggtcac gcattaaccc acgcgtccac
     1441 tttctttctg ggctcatcgt cgtcgcgagt ggcattgcct ccgccgtttt cgtcgtcacc
     1501 gccaatgctt ggatgaatgc gccgagcgga ttcgtgcttc aagagggacg tctctctcaa
     1561 attaatccgc tggcgcctct gtggcatccg gtcgcctggc acgaagcagg acacatgctg
     1621 cttggggcgt ttgctgcgac tggatttctc gtcgccgcca tccatgcatt ctggctcttg
     1681 aaggaaccag acagtgcatt tcatcgcctg gctttgtcga tcgcccttgc cattggaggg
     1741 cctcctgcgc tcttgcagcc gctgagcgga gacttgatcg caaaatcggt ggctgtccac
     1801 cagccggcca aacttgcatc catggaagcc ttgttcgcca cgaagaccgg tgcggaattc
     1861 gtgctcggtg gacttcccga tgctgcgaca cgaaccgtgc cctatggact cctgatcccg
     1921 agtgggctca gcttgcttct ctatggagac gccaacgccc aggtcatcgg gttggatcat
     1981 ttcccccctg aagactggcc tcctgtggcg gtcgttcatg tcgctttcca aatcatggtt
     2041 ggttgcggac tgttgatggc gacaatcgcc gcatggagcg gctggagatg gtggagagcc
     2101 ggacggttga agctcgatcg tccgtggctc ctgaccttga ttgcctctgg tccgttaggt
     2161 ttcgtggcga tcgaagcggg ctgggtcgtg actgaagtgg ggcgacagcc atggatcatc
     2221 gatcatgtgt tgcgcactgc tgatgccgtt acaccaatgc ctggactgtg gattccaatg
     2281 gttgtcttta cgctcctcta cgtcgtgctc gcgggagtcg tgctttgggc tatttggagg
     2341 cacatcgcgg ccacctatct gtcacccacg tgagctggcg agaactgatt cgcatgccca
     2401 ggaaacgagg tgaaaaggag catagaggtg tggttcaaga gccggcctgc ctttcagctg
     2461 gccggaacgt gcggaacact cggtgaggac ttgatgtgat ggggaccctt gaagtccttc
     2521 taggcgcgtc actagtaggc gcgttgacct gttatctcct gacgggagga gcagactttg
     2581 gcgccggcat ctggacgcta tttgcatgtg gccgaaacgg ccagaggcaa cgtgcgttaa
     2641 tcgatcaggc tatcggtccc atctgggaag ccaaccatgt ctggctgatc gttgccgtga
     2701 ccatcctgtt cactgccttt ccacccgcat tttccgtcat tgcgacaagc ttgcacattc
     2761 cgttgtctct catgttggtc ggtatcgtgt tacgaggaac cgcctttgcg gtccggacgc
     2821 atgacattac ctcacgtgat ggcagcagtt ccgtctcacc cctatggcga catctgttcg
     2881 ccgtctccag catcatcacc cctgcgctcc tcggtatcag tgtcggagcg gtcgcctccg
     2941 gcaggctccc ggtagcctct tcaccctcca gttccttcgt cgaacgcttc attgcaccct
     3001 ggttggcagc ctttcccctg agtgttggac tcctcaccac caccctggtg gcttatctgg
     3061 ctgccgtgta cctgctggtt gaatcgcagg accccgagct tcgaaatgcg ttcaggcgtc
     3121 gagcccaagt cgcatgcgtg gccttagtgc tgaccggtgc ctacgcgctg gtccaagctc
     3181 gccagggggc tcccgagatt tacgccggcc ttcgccacac gccagccggc ctgctggcaa
     3241 tcgtggtgac attcggattg cacatggcgg ctctggttac cctatggaaa gctcgcgatc
     3301 gcgcggccag gatcttcgcc gtggccgggg ccgtaattat gctgtggggc tgggccctct
     3361 cccagtatcc ctatctcgtc gagccgagca tcaccatcgc cgatgcggcc cctactgaaa
     3421 cgctccgctt actgctcgtc agtctcattt tggggtcgat catcctgttc ccactccttt
     3481 attatctcta tcgcattttc aaagggcgcg tcgtatttga catctaaaca gagccttaca
     3541 gtgcgtgaca ctacgatgga ggtgagctat gtccacaacc ctcgttcgct ttttgaccgg
     3601 agcgatcgtg ctggcggcgg tctgttggtc gatcttttct tggcaacacc ctgttgcgca
     3661 aggtcaggag gccaaacgct acgcgtacaa ggtagtcgac gtaccgcccg acaatacggc
     3721 catgcaaaat attctcaatg agtatggcgc cgccggatgg gaattgattg tcatcggaat
     3781 gggagacatg acctcgccgc gtttggtatt caaaaagtaa aagggcaccc ccccggctaa
     3841 gacacactgt ctatgcgggg acagcttgtc gtgctgccac gacgcttcgc caagagacgt
     3901 gcagctgggg aatttgacgc ccgtctctcc caggaagcgt ttccgtcgtt tggaagtggt
     3961 aggtctgtgc tttgcacaac aatacctgat tcgcatacac gagtcttggt ctgtcttttg
     4021 cgtgctgagc acctaccatt ccctgccctc gaaaggaacg ttatgcacca ccatgatcac
     4081 gcgccacatc cacacctgca acactgcatt gatacgtgtg cggcttgcgc tcagatctgc
     4141 gatcgctgcg ccgacgatat gatcggaatg gatggcggcg acgaccggga gcttcgagaa
     4201 ctctgcattc gtctttgccg ggattgcgcc gatctttgcg cattatccgt acgctggatg
     4261 agccggcttt cgcctgcgac cgagttgctg tgtcgaacct gctccgatat ctgtgatcgg
     4321 tgcgcgagcg tgtgcgagca acactcgtcg catcatccac tttgcggtga ttgtgcggtg
     4381 gaatgccgcc gttgcgccgc agcgtgccgg gacatggcga cagcagcggc ctaaccgccc
     4441 aataaggagg agccatgctt cgaatcaagg acgtgatgac cccacaggca gaagtgattt
     4501 cccccgatgc cactgctgag gacgcagctg caatcatgaa gaccttggac ataggggtcc
     4561 tcccagtatc cgatgagcaa gggctggtgg gaatattaac ggatcgagac ctcgtcgtgc
     4621 gtgtcatggc tgcgactcgc gatccgaagg ctatgctggt cggcgaagcg atgaccccct
     4681 cggtcgtcta ttgttttgaa gacgacgaca tcgagcaggc cgccacagtg atggcaggac
     4741 aacagattcg gcgtctgccg gtactggaca agaaccggaa attagtcggt atcgtttcaa
     4801 tgggcgatat cgctgtccat gggcgcgagc cgcagctcag cgggaaagtc ttggaggatg
     4861 tgtcgcaacc ggcaacaccc aatcggagcg accaagccaa acgcgagagc ggattcggtc
     4921 aagcggcatg aactgagatg tctatgctcc cgagttcacg tgctatccta gctggagcat
     4981 tggggttgac cctgacaatc gggtgggctc actttatcct ggctgcggac cccccaagtc
     5041 cgacccctgg tcccacgttt cccaatccac agcctactcc gccttctccg gctccagtgc
     5101 cccatccgac gccgccaact cccggtccga ctcagcccac accatcgccg gcgccgacac
     5161 agccctaact tgggacgaca aactaaatgc tccacgtgac ctaatcgccg gatgtttatg
     5221 aagcagctca cttactggga atcagtgtcg atcggatgga ggattctctg gcaaggcatc
     5281 ggcgggttcg tcctggcgct gtttctcggt agcttggcgg tggtcggtct gctgcccgag
     5341 ctgacacgca ccggtccgtc gtacggggca cttgtcgttc ccctggtgag cgcttctgtg
     5401 ctgtcgctgt ttgtgatgtt gccgttggtc gttcgaggcc tgatcaacgc atcctttggg
     5461 accttccggc ttgttgtcat gcgcaaggag ccctgccaat ccaccgtcta ggtaggagga
     5521 ggcactatga cccgtcccct cacacccaca atcgtgctga tgctactctt gaccgccggt
     5581 gcactgaca
//
LOCUS       DASTQY010000422         9634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_969368, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000422 DASTQY010000000
VERSION     DASTQY010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9634
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969368"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..961
                     /locus_tag="VFR82_13635"
     CDS             287..961
                     /locus_tag="VFR82_13635"
                     /inference="COORDINATES: protein motif:HMM:NF021511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEU4502690.1"
                     /translation="MFRRSVSTPSERRGVTQNKRFLLGLLLWYVALSVWTAYEPADRQ
                     FWMLSSILPGLLVLVLVGTYRCLPLSPISYVLITSFLTLHTVGVHYTYAQVPAGAWFE
                     QAVPLGRNEYDRVVHFCFGFLLTYPIQELFRLTIPLRGWLLYYLPVMTVLGLSGLWEI
                     IESWVAQAVHPELGITYLGSQGDAWDAQKDMAAAMYGALLCMALLFFWRKQRPGASRP
                     LAGLES"
     gene            980..1585
                     /locus_tag="VFR82_13640"
     CDS             980..1585
                     /locus_tag="VFR82_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEU4502691.1"
                     /translation="MKKTTLLYGLLAWYGLLWTALAVAPRDRHDWLLENLLALAAVAT
                     LVVTYRRFQFSTLSYLLITAFLSLHAVGAHYTYAEVPLGFWLQHGFGLSRNPFDRVVH
                     FAYGLFLAYPLREILVRLAGVKGFWSYYLPLSGMLAQSGFFEIVEAIVAQIVSPELGA
                     AYLGTQGDEWDAQKDMLAAGSGALLCMMAVIVAPTQSGKTA"
     gene            complement(1692..2369)
                     /locus_tag="VFR82_13645"
     CDS             complement(1692..2369)
                     /locus_tag="VFR82_13645"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEU4502692.1"
                     /translation="MRLLITLIVSLLLNAASAMPARAADPATEDQKTFYALGLIISQS
                     LAPFALSESELELVRNGMTDGVLKKTSKVDLSVYGPKVNQVQQTRSAALAESEKKAGA
                     AFVTKAAAESGAKKTESGAIVTTIKEGKGEQPKATDTVKVHYHGTLIDGTVFDSSVKR
                     GEPATFPLNQVIKCWTEGLQLMKVGGKSKLVCPSAIAYGDRGSPPTIKPGATLVFEVE
                     LLEIVKK"
     gene            2529..3941
                     /locus_tag="VFR82_13650"
     CDS             2529..3941
                     /locus_tag="VFR82_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEU4502693.1"
                     /translation="MVLIFVIIYLLISVSIGLLAATRVHNTKDFAIAGRSLPVPIVMA
                     TVFATWFGAEAVMGISATFAKEGLRGVVADPFGSSLCLVLAGLFFAPRFYRLNLLTVG
                     DYYRLRYDRRVEVLCTLCIVASYLGWVAAQFKVLGLVLNVVTEGAVSQALGMVIGALI
                     VLAYTTFGGMFSVAILDFVQLSVIVGGLLYIANVVGDLTGGVGVVIRHAEQAGKLEFF
                     PPASLNAWIPFVGAGVTMMLGSIPQQDVFQRITSAKDERTAVRGSLMGAALYFAFCFV
                     PMYIAYSALLVDPTKFGTLLERDFQLVLPTLIVEHTPVFAQVMFFGAVLSAVMSCSSA
                     TLLAPSVILSENVIKGAMPRMDERGLLTTMRIVLVCFAFVVLTIALSSDSSIYKLVVN
                     TYKVTLVAAFVPLCAGLFWKRATSGGAVCAIAAGLGCWMGFELLGTAASIWPPQLAGL
                     LMAMVGMIAGSYFFPDVSSR"
     gene            4003..6312
                     /locus_tag="VFR82_13655"
     CDS             4003..6312
                     /locus_tag="VFR82_13655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU4502694.1"
                     /translation="MPSWNRILRSPWLFRGGLFVAGSLVLLVTGLLVYGAYLATFLEL
                     PRNEDHPALRLYTAPFQLKTGLSLKVARLPERLQRLGYRAVKAPVVSSGDYQLTPEAL
                     TIFLHAQPETFVKANVVTLLLEDGVVTQVLSEPDRTPIFPVFLEPELISGLRGSSRQV
                     REWIPLSQMPPRLVEAILAVEDRRFYSHPGIDPMAVGRAVWRNLTKGGVVQGGSTITQ
                     QLAKNLFYTPQRTFARKVKEALAAIVLETKYRKQDILESYLNEIYLGQAGFVSIYGVG
                     EAAHRYFGKNLGELTTGEMAMIAGLVKGPNTFAPTKHRALAQQRRDVVLRLLRDQGHV
                     TEDEWKVAVNQPLQVAPPDDVLADAPYFLDTVLRQVEEAIGTPLPEGLRIDSTLDAIL
                     QQIATEGLENGLARLERAYPALRGSDQLLQGAVAVLDPSTGAILALVGGRDYRVSQFN
                     RAVQAHRQAGSLFKPFVYLAAFEASRTGTAPYMTPASLLADEPVTFESEGATWAPQNY
                     DRQFRGNVTLRSALEQSLNVPAVRVAKVVGTQPIVDVLRQLGVTSALTNDLSLALGSP
                     AVSLLDMVTAYGVLANRGIWVHPTSLRAISDRQGLVLWTPAPDRRQGVSPQAAYLVTS
                     LLEGVVQRGTAAKAKVLGLTGSIAGKTGTTDGYRDAWFVGYTSELVIGVWVGFDDERA
                     IRLTGAQAALPIWMDITRRMLPEHIGPFTPPSGIVARAIDPKTGQLATSQCPEQVEEV
                     FIEGTEPTVYCEVHGGGIWERLRHRFGFS"
     gene            6425..6868
                     /locus_tag="VFR82_13660"
     CDS             6425..6868
                     /locus_tag="VFR82_13660"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU4502695.1"
                     /translation="MKKNGFGGDDNITLLAKGVHLKGEIRVEGTVRIDGRLEGEIHTK
                     GQVIVGEDGVVQGLVMAGTVVSSGRIKATVTAVERVQLLKTAILIGEVHSPVLMMEEG
                     AKMQGTTDMGVAAWPDELPKLAGTVRELVSHRTKHAVVVEQDSNS"
     gene            complement(7032..7625)
                     /locus_tag="VFR82_13665"
     CDS             complement(7032..7625)
                     /locus_tag="VFR82_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEU4502696.1"
                     /translation="MDRFRVAHSTAGLTFLILAVWWVASAYSAKDESKTPGISPERVA
                     GFVHAVLQAHRTIYTTHIVNRLQEKGVVMAVEHWEQENALPLPAQFLQHSGRLVAESG
                     QGIRYRLIGLSPIYQRNAPATDFERKALEFLNRAPEAPITGMVSSGRKQYFQAIYPDR
                     AVSSACINCHNSHPLSPKRDFKINDVMGGIAITIPIE"
     gene            complement(7577..>9634)
                     /locus_tag="VFR82_13670"
     CDS             complement(7577..>9634)
                     /locus_tag="VFR82_13670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4502697.1"
                     /translation="VVRGATKMLFVTDTQGHLVAALTDRPPFANGEFPWWKGTFNKGI
                     GQLYIEDVYFDELADTYAFSISLPVMDSLRYEAVGVIHRVIDAKEFFAPSTHPIRFGK
                     TGHVMLIDHRGIVVSCPILPTGVRLSDASIIPLVTQRQSGWTSAPSDGHGGRSSSIIG
                     FSPLPETSRNTNGSLDVGSWHTFVWQSSDELFGPIKHLFTWMTVFGSIAVALLATLGY
                     LAAMRIVTPVRQLQQAAQSIGRGELRQSIEIKTGDELETLAEEFNRMNRQLEAAFTGL
                     TDQVTMKTQEVQYLQRSTDQILDAVPTPILIVDDQEQVLYVNRTGREAFALTEESSAG
                     GRLFDILPVEVSQQQQLRQELWSHQTTGMPSRLASETPAAVENIRDPLVPETATVHTS
                     RQAELQLGAHLYRYEWFRVSGRPGQSDRVGLVLRDTTDESRMQEKLVQAEKSGSLGLL
                     TAGIGHELNNPLFGIMGLGEALQDDCDPARVKSYAQDIIQHGRRMAAIIRDFTGIAAR
                     EISDQRQTVHLEREIDQALAAITVGLDTSQLAIERNYAGDTVVMALPDQLKQAFSNIM
                     TNALHAMKNQGTLRLSTTVGEKAVVATIADTGPGIPKHHLSRIFDPFFTTKGQGEGSG
                     LGLTVARRIIRKFGGDIRLESVEGAGTSCMVTLPLIAPASSSKEASWTASESRTLPQV
                     SRS"
BASE COUNT         1860 a         2690 c         2841 g         2243 t
ORIGIN      
        1 cgtgaaaacg gtcagcacct atcgaatgcg actgctgtcg aagctgcaat tgaagacgac
       61 atctgccctt attcgttatg cactgaccca tcatctctag cgggcgtgca agccctcctc
      121 gcgcctcaat gtgaccatcc aatcaagtca caggaccacg gctcgttgtt ttgccttgtc
      181 agagcttagg agtcacggcc gcacactctg aggccctgcc cccttaccgc cggtaagtca
      241 gatcctaggt tgcatcaact ccgatgcacg ctgtacattg acggccatgt tcagacgatc
      301 tgtctcaact ccctcggaga ggcgtggggt cactcagaat aagcggttcc tcctaggtct
      361 gctgctttgg tatgtggcgc tttcggtctg gacggcctat gagccggctg atcgacaatt
      421 ctggatgctg tcgagcatcc tgcccggcct tctggttctg gtgctcgtgg gcacctatcg
      481 ctgcctgccg ctttcaccaa tatcgtatgt attaatcacg agttttctga ccttgcacac
      541 agtcggcgtc cattacacct atgcgcaggt cccggcggga gcctggttcg aacaggcggt
      601 tccgctcgga cgcaatgaat acgatagggt ggtgcatttc tgtttcggtt tcttgctgac
      661 ctatcccatt caagaactgt ttcgcctgac aattccgctt cgcgggtggc tgctgtatta
      721 tctgcccgtt atgaccgtcc tgggtctcag tggtctgtgg gaaatcatcg aatcgtgggt
      781 cgctcaagcc gtccaccctg agctgggcat tacgtacttg ggctctcagg gcgacgcatg
      841 ggatgctcag aaagatatgg cggcggcgat gtacggagca ttactgtgca tggccctctt
      901 attcttctgg agaaagcagc gtccaggtgc ttcacgcccg ctcgccggac tcgaatccta
      961 ggtaacagga atagttgtgg tgaagaagac gacgctgctg tatgggctgt tggcctggta
     1021 tggcctgctc tggaccgcac tggccgttgc tccccgcgat cggcacgact ggctgcttga
     1081 aaacctctta gcgctcgccg ctgtcgcgac cctggtcgtc acgtaccggc ggtttcaatt
     1141 ttcaacactg tcgtatctcc tcattactgc cttcttaagc cttcatgctg tcggcgcgca
     1201 ttacacgtat gcggaagtcc cgttggggtt ctggttacag catggattcg gactgtcccg
     1261 taatccgttc gatcgggtgg tccatttcgc ctacggactg ttcctcgcct atcctttgcg
     1321 cgagatcttg gtccggctgg caggcgtaaa gggcttctgg tcctactatc tgccgctcag
     1381 tggcatgctc gcccaaagcg ggttctttga gattgtggaa gcgatcgtcg cccagatcgt
     1441 cagtcccgag ttgggtgcgg cctatctggg gacccaagga gacgaatggg atgcccagaa
     1501 agacatgctt gccgccggtt ccggtgcact gctgtgcatg atggcggtga tagtcgcacc
     1561 gacgcaatcg ggaaagactg catgagcgtg gcacaccgcc ttccgagggc gacgccgcgg
     1621 ctggccagcc accgccttca tgctgatggc ggatgagcta gccgctggag tcgacagaaa
     1681 acggaacgga gctacttttt cacgatctcc agcaattcca cttcaaagac caatgtggct
     1741 ccgggcttga tcgtcggcgg tgaaccgcga tcgccgtacg cgatggctga gggacagacg
     1801 agcttgctct tgccgccgac cttcatcagc tgcaggcctt ccgtccagca tttgatgact
     1861 tgattcagtg gaaacgtcgc cggctcaccg cgtttgacgg agctatcaaa gacggtcccg
     1921 tctatgagcg tgccgtgata gtgcaccttg acggtatcgg tggcttttgg ctgctcacct
     1981 tttccttcct tgatcgtcgt gacgatcgcc ccggattcgg ttttcttcgc tcctgactca
     2041 gcggccgcct tggtgacaaa tgcggcgccg gctttctttt cactctcggc cagggctgcg
     2101 gatcgcgtct gctggacctg attgaccttt gggccataga cggaaaggtc gaccttggac
     2161 gttttcttga ggacgccgtc ggtcattcca tttctgacga gctccagctc ggattcgctc
     2221 aaagcgaacg gggcgaggga ttggctaatg atcaaaccga gcgcataaaa ggtcttttga
     2281 tcctcggtcg caggatcggc ggcgcgggcc ggcatggctg aggcggcgtt gagtagtagg
     2341 ctcacaatga gggtgatcag gagacgcatg cacacatcct ttctgtatgg aggaatcggc
     2401 cttaatgctg tgcttcagta cagtaagcca tcagcgatgg cgggctcaag acaattaatt
     2461 cccgggttgc gcgtttgtcc gtgcaacggt acactgcgct tccagcctca tccactatcg
     2521 actcctgaat ggttctgatt ttcgtcatta tctatctcct gatttcggtc agcatcggtc
     2581 ttcttgcggc gactcgcgtc cacaatacca aggatttcgc gatcgccgga cgcagtctcc
     2641 ctgtgccgat cgtcatggca accgtctttg cgacttggtt cggagcagag gcggtcatgg
     2701 gcatttctgc gacgtttgct aaagaaggac tgcggggcgt ggtggcggat ccattcggct
     2761 caagtctctg tcttgttctc gcggggctgt tctttgcgcc tcgcttctac cggctcaatc
     2821 tgttgactgt cggtgattac tatcgactcc gctacgaccg acgggtggaa gtgttgtgca
     2881 cgctgtgcat cgtggcatcg tatctggggt gggtcgctgc acagttcaaa gtgttagggc
     2941 tcgtactaaa cgtggtgacc gagggagcgg tcagccaggc cctgggcatg gtgattggtg
     3001 cactgatcgt gctcgcatat acgacattcg gtgggatgtt ttccgtggcg attctggatt
     3061 ttgtccagct ttccgtgatc gtgggtggcc tgctctatat cgccaatgtc gtcggagacc
     3121 ttaccggtgg agtcggcgtc gtgatccgcc acgcggagca agcgggaaag ctcgagttct
     3181 tcccgcccgc ttctctaaat gcatggatcc cttttgtggg cgctggtgtg accatgatgc
     3241 tgggctcgat cccgcagcaa gatgtgtttc agcgcattac gagcgcgaag gacgaacgga
     3301 cggcggttcg tgggtctctc atgggcgcgg cgttgtactt tgccttttgc ttcgtcccga
     3361 tgtatatcgc ctattctgcc ttgcttgttg atccgacaaa gtttggaaca ttgctggaac
     3421 gtgacttcca gctggtcctg ccgacgttaa tcgtcgagca tacgcccgtg tttgcccagg
     3481 tgatgttttt cggagcggtg ctctcggcag tcatgagttg ttcgagtgcc accctgctgg
     3541 cgccctcggt catactgagc gaaaacgtca tcaaaggcgc gatgccgcgc atggatgaga
     3601 gggggctcct gaccaccatg cggatcgtgc tcgtgtgctt tgccttcgta gtgttgacca
     3661 ttgcgctcag ttcagattcc agcatctaca aattggtggt gaatacttac aaggtgactc
     3721 ttgtggcggc ctttgtcccg ctctgtgccg gcctgttctg gaaacgcgcc acgagcggag
     3781 gcgcggtatg cgccatcgca gcgggcctgg ggtgttggat gggttttgaa ctgctgggta
     3841 ccgcagcatc catctggcct ccgcagcttg caggattact gatggcgatg gtcgggatga
     3901 tcgccggctc atacttcttc ccggatgttt cctcacgata agacctttgt gggccgtggc
     3961 tatcaatcgc tttctcttgc ggcctcgcgt atgctgaacc ggatgccctc atggaatcgc
     4021 attctgcgtt caccctggct tttccgtggt gggctgtttg tggccgggag tcttgtactg
     4081 ctggtgaccg gccttctggt ctatggggcg tatctcgcca ccttccttga gcttcctaga
     4141 aatgaagatc atccggcgct acggctatac acggccccat tccagctcaa gaccggtctc
     4201 tcgctgaagg tcgcacgcct tccagagcga ttgcagcggc tcggctaccg agcggtcaag
     4261 gcaccggttg tctcgtccgg tgactatcaa ctcacgccag aggcgctcac catttttctg
     4321 cacgcccagc ctgaaacttt cgtgaaggcc aatgtggtca ccctcttgct ggaagacgga
     4381 gtggtcacac aggttctatc tgagccggac agaaccccca ttttcccggt ttttctcgag
     4441 ccggagctga tcagtggatt acgcgggtct tcgcgtcaag tacgcgaatg gattcccttg
     4501 tcccagatgc cgccgcgctt ggtcgaagcc attctggccg tggaagatcg gcggttctat
     4561 tcgcatcctg ggatcgatcc gatggcagtc ggacgggcgg tctggcgaaa cctcaccaaa
     4621 ggcggagtgg tacaaggcgg cagtacgatt acgcagcagt tggcgaagaa ccttttctat
     4681 acacctcaac ggacattcgc acgaaaggtt aaggaagcgc ttgctgccat cgtgctggaa
     4741 acgaaatacc ggaaacagga cattctcgaa agttatctca atgagatcta tctcggccaa
     4801 gccggattcg tatcgatcta tggggtcggg gaggcggcac atcgctactt cggtaagaat
     4861 ttaggtgagc tcaccacagg cgaaatggca atgatcgcgg ggctggtcaa ggggccgaac
     4921 acgtttgcgc cgacaaaaca ccgcgccctg gctcagcaac gtcgcgacgt cgtcctgcgt
     4981 ctgttgcgcg atcaaggcca tgtgacggaa gacgaatgga aggtcgccgt caatcaaccg
     5041 cttcaggtgg cgccacccga cgatgtcttg gctgatgcgc cgtatttcct cgataccgtt
     5101 ctgcgccaag tcgaggaagc tatcgggacg ccgttgccgg aaggcctgcg aatcgacagc
     5161 acgctcgatg caattctgca gcagattgcc acggaaggcc tcgagaacgg actggccaga
     5221 ctagagcggg cctatccagc cctcagagga tccgaccaac tgttgcaggg ggcggtggcg
     5281 gtactggatc cctccaccgg cgcgattttg gcgttagtgg gcggacggga ctaccgcgtc
     5341 agccagttta atcgagcagt acaagctcac cgacaagccg gctcgctctt caaacctttt
     5401 gtctaccttg ccgcgtttga agcaagccga actggcacgg caccctatat gacaccggcc
     5461 agcctgctgg cggacgagcc cgtaactttt gaatcggaag gtgcgacatg ggcgcctcag
     5521 aactacgata ggcagtttcg agggaatgtg acgctgcgta gcgcgttaga gcagtccctc
     5581 aacgtgccgg cagtgcgtgt ggcgaaggta gtcggcacgc aacccatcgt cgatgtcctc
     5641 cgccagttgg gagtgacaag cgcgctgact aacgatctgt cgcttgcctt gggtagtccc
     5701 gcggtctcgc tgctcgacat ggtgacggca tacggcgtgc ttgccaatcg cgggatttgg
     5761 gtccatccca cgagcctacg tgccatatcg gatcgacagg gcctcgtgct gtggacaccg
     5821 gcaccagacc ggcgccaagg cgtatctcct caggcagcct atctggtgac gtcgctgctt
     5881 gagggtgtag tgcagcgagg gaccgctgcg aaagcgaaag tattgggctt gaccggatcg
     5941 attgccggca agaccggcac gaccgatggc tatcgtgacg cctggttcgt aggatatacc
     6001 tcggagcttg tgatcggcgt ctgggtggga ttcgacgacg aacgagccat ccgtttgacg
     6061 ggtgctcaag cggcacttcc aatatggatg gacatcacga ggcggatgct gccggaacac
     6121 ataggtccgt ttactccgcc gtccggcatt gtcgcgcgag ccatcgatcc caagacggga
     6181 caattggcga cctcgcaatg tccggagcag gttgaggaag tcttcattga aggaactgag
     6241 ccaaccgtgt attgcgaagt gcacggcggc ggcatctggg aacgtctccg gcacaggttt
     6301 gggttttcgt aacgggttcc tgagcgcatc tctacatccc acctcagtac cctttcaaca
     6361 ttctcctagg gcttctggtt cgaatggtgt aagcttggcg gtgagctgta gtgggaggga
     6421 ccgcatgaaa aagaatggat tcggtggcga cgacaacatc acactcttgg cgaagggcgt
     6481 gcatctcaag ggagagatca gagtggaagg gacggtgcgg attgacggcc gtcttgaagg
     6541 agaaatacac acgaaaggtc aagtcatcgt cggcgaagac ggcgtcgtac aaggtcttgt
     6601 catggccggg accgttgtga gcagtgggag gatcaaggcc accgtgacgg cagtcgagcg
     6661 cgtgcaattg ttaaaaactg ccatcctgat cggggaagtg cattcgccgg tgctcatgat
     6721 ggaagaaggt gccaaaatgc agggtaccac cgacatggga gtcgcagcct ggcctgacga
     6781 actgccgaaa ttggcgggca ccgtccgtga gctggtgtca cacagaacta aacatgcggt
     6841 agttgttgag caagactcca attcatagca cgcctgctgt ccagcgtctc gcatccaacg
     6901 cgagggcgaa gaactgagcc attgaggatc ctgcgagcca acccatccta gaggcgattc
     6961 tcttcctctc ccaaggccgc aagcgtttgc ggccggcttt ttccctcctc atttctctgc
     7021 tgtagcgggc ctcactcgat cggtatcgtg attgcgatac cgcccatcac gtcattgatc
     7081 ttgaaatccc gtttagggct cagcggatgg ctgttatggc aattgatgca ggcggaggat
     7141 acggcacggt ctggatagat ggcctggaaa tactgtttgc gtccgctgct caccatcccc
     7201 gtaatcggtg cctcaggggc acggttcagg aattccagcg ccttccgctc aaaatccgtc
     7261 gccggggcgt tgcgttgata gatcggcgag agcccgatga gacggtagcg gatcccttgc
     7321 ccgctttcgg ccaccaaccg cccggagtgt tggagaaatt gcgcgggaag cggcaaggcg
     7381 ttctcctgct cccaatgctc gacggccatc acgaccccct tttcttgaag gcgattgacg
     7441 atgtgcgtcg tgtaaatcgt ccggtgcgcc tggagcacgg catgcacgaa acccgccacc
     7501 ctttccgggg aaatgccggg ggtctttgac tcgtctttgg cggaataggc gctagcgacc
     7561 caccagaccg ccaggattag gaacgtgaga cctgcggtag agtgcgcgac tcggaagcgg
     7621 tccatgatgc ctcctttgat gaagaggcgg gagcgatgag cggcagcgtg accatgcagg
     7681 acgtgccggc tccctcgaca ctctccaatc gaatgtcacc gccgaatttc cttatgatgc
     7741 ggcgtgccac cgtcagtccg agaccggagc cttcgccttg gcccttggtt gtgaagaatg
     7801 ggtcaaaaat cctcgacaga tggtgcttgg gaatgccagg acctgtatcg gcgatagtgg
     7861 cgacgacggc tttctctccc acggtcgtcg acagccgcaa cgttccctgg ttcttcatgg
     7921 catgaagggc attcgtcatg atgttcgaga atgcctgctt cagttggtct ggcagagcca
     7981 tcactaccgt gtcgccggca tagttgcgct cgatggctaa ctgcgacgtg tccaagccga
     8041 ccgtgattgc cgcgagcgct tgatcgattt cacgctccaa atggaccgtc tgtcgctgat
     8101 ccgatatttc gcgggccgcg atgccggtaa aatctctgat gattgccgcc attcgcctgc
     8161 cgtgctgaat gatgtcctgg gcataggatt ttaccctcgc gggatcgcag tcgtcctgta
     8221 gggcttctcc taatcccatt atgccgaaca aggggttatt caattcatgc ccgattcctg
     8281 cggtgaggag gccaagactt ccagactttt ctgcttgtac gagcttctcc tgcatgcgac
     8341 tttcgtccgt cgtgtctcgg agcacgagcc ctacacgatc ggattgtccc ggtcggccgg
     8401 acacacgaaa ccattcgtag cggtacaagt gagcgcccaa ttgcagttcg gcctgtcggc
     8461 tggtatggac agtcgccgtt tctggaacga gcggatctct gatgttttcg acggcagccg
     8521 gtgtttcact ggccaagcgt gacggcatgc cggtcgtttg atgactccac agttcctgcc
     8581 gtagttgctg ttgctgcgag acctcgaccg gcagaatgtc gaacagccgc ccgccggcag
     8641 atgattcctc tgtcagggca aacgcctccc gtccggtccg attcacgtac aacacctgtt
     8701 cctgatcgtc cacgattaaa atcggggtcg gtactgcatc gaggatctgg tcggtagatc
     8761 gctgcaaata ctgcacttcc tgggttttca tggtgacttg atccgtcagg ccggtgaacg
     8821 cggcttccaa ttgccgattc atgcggttga attcttcagc caatgtttcc aactcatcac
     8881 cggtcttgat ctcgattgac tgtcgcagtt ctccgcggcc gatcgattgg gcagcttgtt
     8941 gcagctgtcg aaccggtgtg acgatgcgca tggcggccaa atagccgagc gtcgccagta
     9001 gcgcgacggc gatcgagccg aagaccgtca tccacgtgaa taaatgtttg atggggccga
     9061 aaagctcatc tgatgattgc caaacaaacg tatgccatga tccgacatcc aacgacccat
     9121 tggtattccg actggtttct ggaagcggag aaaagccgat aatggacgac gagcgtcctc
     9181 catggccatc gctcggtgcg ctcgtccagc cggactgacg ctgcgtgacc aagggaatga
     9241 tgctcgcgtc cgagagtcgg actccagtag gaaggatggg acagctcacg acgatgcctc
     9301 gatggtcgat caacatgacg tggccggtct ttccaaaccg gatcgggtga gtggatggag
     9361 caaaaaactc cttggcatcg atcactcgat ggatcacgcc gaccgcttca tagcggagac
     9421 tgtccatgac aggaaggctg atggaaaacg catacgtgtc ggccaactcg tcgaaataca
     9481 cgtcttcgat atacagttga ccgatgcctt tgttgaacgt gcccttccac caggggaatt
     9541 ctccattagc gaacggcggc ctgtcggtca gcgcagccac gagatgccct tgggtatccg
     9601 tcacaaaaag cattttcgtg gcaccacgaa ccac
//
LOCUS       DASTQY010000423        11977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1307027, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000423 DASTQY010000000
VERSION     DASTQY010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11977
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307027"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="VFR82_13675"
     CDS             <1..986
                     /locus_tag="VFR82_13675"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEU4502698.1"
                     /translation="LVMLTKDDGRQTAALFIVRDDTRDAEVGVQLPTATYQAYNNYGG
                     ESLYESSHGLSGGKARKVSLDRPIARQYGNGTGTFLYQELEAVMWLEDHGYDVEYQAS
                     SDTGGANGRIGAHRLFTIIGHEEYATMAEFDRLQSAVANGTSLAFLTGNTLYWQIRYE
                     NNDRVIVGYKDNASEDPMRLTNPSLVTSTFRNPPVNRPENQLVGVMSDGSFITQPSDW
                     VVTNANHWVYAGTGLSNGSRIPNLVFTEWDALVNNGLTPAGITVLASSVVSNNIPAIP
                     SRHESTIYERGNAFVFAAGSIFFSQHIRSQPAVGQIVANLLTRAGAGAHEP"
     gene            1501..2220
                     /locus_tag="VFR82_13680"
     CDS             1501..2220
                     /locus_tag="VFR82_13680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4502699.1"
                     /translation="MISRSSAPLMPSPRTSRLEGRNAEPAPSLVSCRLLLADDHTLLR
                     QGVKALIETYGVTVVGEAGDGREAVRMAEEMQPDVAVIDVMMPLLNGLDVVREVRRVS
                     PATKSLLLTMHTDDAFVLEALKVGVKGYLLKSQPVTDVVHAIRDVSKGSFYLSPGISQ
                     TVIQAFLAKSDIPSDPLTTREREVLQLVAQGMSTKKVAGMLGVSVKTAESHRHRIMDK
                     LDLHDTASLVRYSIRRRLIEP"
     gene            2236..2928
                     /locus_tag="VFR82_13685"
     CDS             2236..2928
                     /locus_tag="VFR82_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4502700.1"
                     /translation="MAKILVVEDMDSVVDLLRSLLQREGFEVAVAQDGPEALEAVRRE
                     KPDLILLDLILPGLDGLEVLRRIRQDPITAHLPVIVLSGKDEERDKVIGLEIGADDYI
                     TKPFQANELIARVKGRLRRSAPTDPARVLKVGSLEMELDRYQVTLRGKPIHLTSKEFS
                     LLRALLMTNGRVLSRDALFESVWGASKDADLQSRTIDVHIRSLRNKLGAEGRRIFTVR
                     NVGYRLDPAPTE"
     gene            complement(2950..3825)
                     /locus_tag="VFR82_13690"
     CDS             complement(2950..3825)
                     /locus_tag="VFR82_13690"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4502701.1"
                     /translation="MAMEIAERSRQDFAEEHVEEVRQGRRFEFGDNWYKFLQNLKEEQ
                     IVSAETSLREMLEISDLTGKKFLDAGSGSGLFSLAARRLGAQVHSFDYDPQSVACTKE
                     LRRRYSHNDPWWRIEHQSVLDKAYLSSLGRFEVVYSWGVLHHTGAMWQALDNVHHSVA
                     AGGLLFIAIYNDTGTQSARWRRIKQLYNALPRPFRWPYTAVVAAPLEFKAVLTALVSG
                     HPSRYIRRWTDKNERGMSHWRDAVDWIGGYPYEVAKPEQIFEFYKARGFSLRRLKSTV
                     GLGCNEFVFRKDRDV"
     gene            4022..5419
                     /locus_tag="VFR82_13695"
     CDS             4022..5419
                     /locus_tag="VFR82_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502702.1"
                     /translation="MLVRTSTVILRAAVCCSLLMLVATAVVGQTTSSFPGGWIEKQGA
                     FTRARYTASQIQSFVPPGRGTFTFPAPYKTKGIRITDASDCGGADCVNYIGYSYWRNT
                     NAHESSADMWIFVGLSTARGGTGPTLFKLNKATDVVTKVGPLFPAGSPFRTQSGETWY
                     FSASRPNKLYINDGPRLLRYDVVSKVSDTVFDITAQFGSGRYVWQTHSSNNDMVHSGA
                     LRVSATGEDLGCFVFNETTRKFSFFPKIGTYDECHIDKSGKYLMIQENTDGKYGLENV
                     FIDLETGKQSIVMDQNGGLGHADMGFGYALGMDNWNSLPGAAITFNLAGTVKGPCVFY
                     SNNWNMNPVNHVSHLNAKPTIPMSQQFACGSNADYGSAQNEVVCFRLDASHDQLIVAP
                     VMTDMNAPGGGSEYAKYPKGNLDISGRYFIWTSNMGGNRLDAFLVKVPSHLLTGSSDI
                     AAPAAPVNLRVTTRP"
     gene            complement(5437..6669)
                     /locus_tag="VFR82_13700"
     CDS             complement(5437..6669)
                     /locus_tag="VFR82_13700"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4502703.1"
                     /translation="MTMIILTWWAMVLLVYTYAGYPALLWLISRLKDPLETERGSTNS
                     WPSLTVVISAYNEEAVIARRIQNLLDQDYPSEQIEILIGSDGSTDATCQVVESYRSAG
                     VQLAAFPTRRGKASVLNDLVRLAGGEMVIFTDAATVFYPDAFRQLVTGFWRYPSASVV
                     VGELEMRSSRSSRNLDGWYWRYELFLKGLESEIGAGLGASGTIYAVRREDYCPLPPDT
                     VADDLLEPLLVRLRTHGHVVQYGAARAWQLIPERVADEFRRRVRSGAGIAHVLRHAWR
                     LLLPQWGVVSLALWSHKALRLLAPWMLLTLLAGAIFLRHYPFYQWLFISQALFYGAAL
                     CAGWLRAVPLVGKASIGARYFVVLNAALALGSLKFIFGMARPTWNRTRRPEEEAARAD
                     DWSEMMPDDAVEKQRPAA"
     gene            complement(6732..7526)
                     /locus_tag="VFR82_13705"
     CDS             complement(6732..7526)
                     /locus_tag="VFR82_13705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4502704.1"
                     /translation="MEHQTALQEWEQAEIRRSAAEAESADASSLLASEDNVARYVNPP
                     ADTLFHLEYAFHLLGDVTGQQVLEYGSGWGENTIILARLGAEVHALDISPELIAINRR
                     RMQVNGVTSGVRFSVASAYEVPCPDDSVDVVFGMAILHHLDLDRAAREVYRVLRPGGR
                     AIFSEPMRNSPTLRALRKMVPCPLPANVSPYERPLTLGELAEFSTGFIAGRSRSFELP
                     WSRAAERIPVLRASLMPLRRWDRAMLARFPGLAYYAAIRVFEVRKP"
     gene            complement(7541..9226)
                     /locus_tag="VFR82_13710"
     CDS             complement(7541..9226)
                     /locus_tag="VFR82_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502705.1"
                     /translation="MTYRSSWMCPFMVAMVMLAIPIGEGCAATASGVRPALPHEYVDT
                     TVKSSPGRTLTVPAGGDFQAMLDEAQPGDEIVLTAGARYKGPFTLPYKKAGSGWITIR
                     SGGIGKDFPAPGTRVQPSQTGRMAALVSNQHGVIIAEPRAHHYRFIGIEIKPEDNILL
                     RDLVLLGDNTARSLDDLPHHIILDRCYVHGDPKKGARRGVVMNGRHLAIVDSYVSGFR
                     EEGQDSQAVVGWGGSGPFKVENNLLEGAGENLLFGGGDPSIRDLVPSDIEIRHNHVVK
                     PLSWRPGEPEHDGGNWQVKILFELKNARRVLADGNIFEYNWQRPGYGFAITFTVRNEE
                     GRSPWAVIEDVTFTNNIVRHSPSGVNIMGSDDIHPPSGQGQRILIKNNIFDDINGPRW
                     GGKGRLFQLLRGTKHVVIEHNVGFHTGHTVMAAGDPHEGFVYRYNITPHNEYGMEGEG
                     TGVGKPTLDRHFPGAIVIHNMLIGGESYAERYPEDNAFPASVEAVPFINAAAGDYRVA
                     ASSAVSGPPTSEVPGIDAKSLCAALGDTGRMEAVCSKPQSKDRGNKSQAAKSE"
     gene            complement(9223..10380)
                     /locus_tag="VFR82_13715"
     CDS             complement(9223..10380)
                     /locus_tag="VFR82_13715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4502706.1"
                     /translation="MIEGQSIICISQDWEGDPTSKKHIMRILSARNRVLWVNSIGMRR
                     PTAAASDLRRLVAKLRRIVSGVRQVEPNIYVMDPPVLPLPGIRAAERVNRIVLAARLR
                     HLCRRLDMPRPIIWTFLPNVNWLLGRLHERMVIYHCVDEYSQFSGVPKDVIIRMEREL
                     IRRADIVFTSAEKLCAERREINPRTHFIPHGVDVAHFSRALDPATAVASEIATLQKPV
                     IGFFGLLADWVDLDMIGALARARPQWSFALVGKAQTDLHAVQGLPNVHLIGQRPYSAL
                     PGYCRGFDVGLIPFRMNELTLRVNPLKLREYLAAGLPVVSTPLPEVVRYQGVVHIATN
                     RESFLREIEAALKERTSERDRGRVEMMRSEGWEARVGEMSSLVEERLMEAA"
     gene            complement(10443..>11977)
                     /gene="asnB"
                     /locus_tag="VFR82_13720"
     CDS             complement(10443..>11977)
                     /gene="asnB"
                     /locus_tag="VFR82_13720"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEU4502707.1"
                     /translation="RLLLARDRLGIKPLYYACNDHELLFASEIKGIVASGIPNVSFNH
                     AILPEFLANRYVAGAETFYRGVLKLLPGHLLEWSAEQGTTTRRYWHPPQLSVNDRAPL
                     SQQADQLRTGLQTAVRSHLMSDVPLGLFLSGGLDSSGLAALMAPMMPQGLKTFSVGFA
                     EGEANELQYARLVAKAVGSDHHEVLVSPEQFMQALPKLVWHEDEPIAFPSSVPLYFVS
                     RLASQHVKVVLTGEGADELFLGYDYRYRVTALNDRIGRFYWSSVPRALRRRISGSLGR
                     LPLRIQRYASRTALAYEPGPRGLFFDNFSVFPESLQRTLLKERSATGPDPYAEGLRYW
                     HEHRGTLLERMSYADVGTHLVELLMKQDQMSMAASLESRVPYLDHLLVEQVMQIPGEQ
                     RMRRWQTKVILREALRNMVPPAVMNRSKMGFPVPFGSWVRGPFRTILDEYVLSTRALD
                     RHLFDPETVRRIVDEHVSGTRAHSDRLWLLVNLEMWQRIAIDGEMPQPLEEQVEAETV
                     PA"
BASE COUNT         2663 a         3552 c         3297 g         2465 t
ORIGIN      
        1 atctggttat gttgaccaaa gacgatggcc ggcaaaccgc cgcgctcttt atcgtgcgcg
       61 atgacactcg tgatgccgag gtcggcgtgc agctacccac ggctacgtac caggcctata
      121 acaattacgg cggcgaaagc ctctatgagt cctcccacgg tttatcggga gggaaagcgc
      181 gtaaagtttc gttggacagg cccattgcaa ggcaatatgg gaatggcacc ggtacctttc
      241 tctatcagga actcgaagcg gtgatgtggc tggaggatca cgggtatgac gtcgagtatc
      301 aagccagcag cgataccggg ggcgcaaacg gtcgaatcgg agcgcaccgc ctgtttacca
      361 tcatcggaca cgaagagtac gcaacgatgg ctgagttcga ccggctacaa agcgcggtcg
      421 caaacggcac gagtctggcc ttcctgacgg ggaacacgct gtattggcaa atccgctatg
      481 aaaataacga tcgcgtcatc gtcggctaca aagataacgc atctgaagac cccatgcgat
      541 tgactaatcc aagcctggtc acgtccacct ttcgcaatcc ccccgtgaat cggcccgaaa
      601 atcagctggt cggtgtcatg tcagacggca gctttatcac tcagccatcc gattgggtcg
      661 tgaccaatgc aaatcattgg gtgtatgccg ggaccggcct gagcaacggg agccggattc
      721 caaatctggt ctttaccgaa tgggacgcac tcgtgaacaa cgggctgacg ccggcaggca
      781 ttaccgtgct ggcttcgtcc gttgtctcga acaatatccc cgccataccc agtcgccacg
      841 aatcgaccat ttatgaacga ggtaacgcat tcgtgtttgc cgccggctca atctttttta
      901 gccagcatat ccgaagtcaa ccagctgtgg gacaaatcgt cgcgaacctc ttgacgcgcg
      961 ccggcgcagg cgcacatgaa ccgtgacaaa gaggtcataa tctgcgtgac aattcgtatg
     1021 acgggtcgtg caggaaaagg attcaagcag ctcggcgggt cgttctcacg gaaggcttga
     1081 tgactggaag agaccaggct cgggcctggc gctcgtagca tacagggcag tatgtatgac
     1141 tcaaccgcac atcgatgaga gtgaggtgat gccgctcaag ataggttgac atcggcgtcc
     1201 aatgaataga ggacgaaacc ggctctgcaa cgtgtttgca aacacagcac acgctcaaca
     1261 taggaacggt tgctgtaatg gttgccacgg gtcctccctt tgtgcggtta ctctaagcaa
     1321 acgatcgcac cggtccaact aggcggttcc ctaggtgcga actgggtagg tcatgtgaca
     1381 gagtgacgca taggtacagt ggacggggat cactctcaat tcaatcagcg agggataatt
     1441 cgcaaggatg tcgaggggtt attacgcgtg ggtgaaggaa ggcaacgaaa ggagttctac
     1501 gtgatctctc gcagttcagc accgctcatg ccgtcgccta gaacgagccg gctcgagggt
     1561 cggaatgccg agcctgcccc atcactcgta tcctgtcgct tgctgcttgc cgatgatcat
     1621 acgctgctga gacagggtgt gaaggccctg atcgagactt atggtgtcac cgtcgtgggc
     1681 gaggcaggcg atgggcgcga agccgtccgc atggcggagg agatgcaacc cgatgtggcc
     1741 gtgatagatg tcatgatgcc attattgaat ggtttggacg tcgtgcgcga agtacgacga
     1801 gtgtcgccag cgacaaaatc gctcttgctc acgatgcaca cagacgatgc cttcgtcctg
     1861 gaagccctaa aggttggcgt caaaggttac ctgctcaagt cgcaaccggt gaccgacgtc
     1921 gtgcatgcga tccgcgacgt gtccaaaggc tcattttatc tgagcccggg catatcccaa
     1981 acggtcatac aagcattcct tgccaagagc gacattccct cggacccgtt gaccacgaga
     2041 gaacgggaag tcttgcaatt ggtcgctcaa ggcatgtcga cgaaaaaagt cgctggcatg
     2101 ttgggtgtca gcgtgaaaac ggcggaatcg caccggcatc gcatcatgga caagctggat
     2161 ctccacgaca ccgccagtct cgtgcgatac tccatccgcc gccgcttgat cgaaccctaa
     2221 atttcaaagc cgctcatggc aaagatactc gttgtcgaag acatggacag cgtcgtcgac
     2281 ctccttcgca gcctcttgca acgcgaagga ttcgaggtgg cagtcgctca ggacggaccg
     2341 gaggcgctcg aagcggttcg tcgtgaaaag cccgacctga tcttgctcga cctgatcctg
     2401 ccaggtctcg acgggttgga ggtccttcgc cgtatacggc aggaccctat caccgctcat
     2461 ttgcccgtga tcgtgctgag cgggaaggat gaggagaggg acaaggtcat cggactcgag
     2521 atcggtgccg atgattacat cacaaagccc tttcaagcca acgagctgat cgcccgggtc
     2581 aaggggcggc tccgccgatc agctcccact gatccggctc gcgtgctgaa ggtcggctcg
     2641 ttggaaatgg aactcgatcg ttaccaggtg acgcttcgcg gaaaaccgat ccacttgacg
     2701 tcgaaggaat ttagcttact gcgcgcgcta ctgatgacga acggtcgcgt cctgagccgg
     2761 gatgcgctct tcgaatcggt atggggagcc agcaaggacg cggatctgca gtctcgtacg
     2821 atcgacgtcc acattcgcag tcttcgcaac aagctcggcg cggaaggtcg ccgcatcttc
     2881 acggtccgta acgtaggtta ccgtctcgat cccgctccca cggaataact tcttgggccc
     2941 atcacgcctt tacacatccc tgtccttccg gaagacaaac tcgttgcatc ccaatcctac
     3001 ggtggatttc aatcggcgga ggctgaagcc tcgggccttg taaaattcga atatctgctc
     3061 cggtttcgcc acttcgtacg gatatccgcc tatccagtcg accgcatcgc gccaatgact
     3121 cattcctctt tcgttcttgt cggtccagcg cctgatatag cggctgggat gcccggaaac
     3181 caacgcggtg agaacggctt tgaattccaa tggtgcggca acaaccgccg tatagggcca
     3241 ccggaaaggg cggggtaaag cattgtagag ctgtttgatg cggcgccacc gcgcactctg
     3301 cgttcctgta tcgttataga tcgcgataaa tagcagacct ccggcagcta ccgagtggtg
     3361 cacgttgtcc aatgcctgcc acatcgcacc cgtatggtga agcacgcccc aggaatacac
     3421 tacctcgaac cggccaaggg aggagagata agccttatcc agtacagatt gatgctcgat
     3481 tcgccaccac ggatcattat gtgagtagcg tcggcggagt tccttcgtac aggcgaccga
     3541 ttgcgggtca taatcgaatg aatgtacttg agcgcccagc cgacgcgccg ccagactgaa
     3601 tagcccgctg ccggaccccg catcaaggaa tttcttaccg gtcaagtccg agatctccag
     3661 catctcacga agcgacgtct ccgccgacac aatctgttct tccttcaaat tctgcaggaa
     3721 cttgtaccag ttatccccaa actcaaatcg cctcccctgt cgcacctctt cgacatgttc
     3781 ctctgcaaaa tcctgtcggc tccgctctgc gatttccata gccatccctc cctcgctcct
     3841 actctctgct gtcgtccgat tgttcgattg aatggcgcgc ctctcgatgg cggtcctcac
     3901 gattttaccg gctcttcatg tagttctttc catctactga cattgcattt acgcatcttc
     3961 actgaggctt tgtctgacga tgataccgtc ctttggagaa ccacctacca ggaggaaggc
     4021 tatgctcgtc agaacaagta ctgtaatcct tcgtgctgcc gtatgttgtt cgctcctgat
     4081 gctcgtcgcc accgcggttg tggggcaaac aaccagttcg tttcctggcg gctggatcga
     4141 gaaacaaggc gcgtttacgc gagctcgata cacggcatca cagatccaat cgttcgtccc
     4201 tccgggacgc ggcaccttca ccttccccgc cccgtacaaa accaaaggca ttcggattac
     4261 ggacgcgtcc gactgcggcg gcgcagactg cgtcaattat atcgggtact cctattggcg
     4321 aaacacgaac gcgcacgaaa gcagcgctga catgtggatc ttcgtagggc tgagcaccgc
     4381 caggggcgga acaggtccga cgctgtttaa gctcaataag gcgaccgatg ttgttacgaa
     4441 ggttggtccg ctttttccgg cggggagccc atttagaacc caatccggtg aaacatggta
     4501 tttcagcgcc agccggccca acaaactcta catcaacgac ggcccaagac tgctgcgcta
     4561 tgatgtcgtc tcgaaggtct ccgacacggt cttcgatatc acagcacaat tcgggagcgg
     4621 ccgctacgtg tggcagacgc attccagcaa caacgacatg gttcacagcg gggctttgcg
     4681 cgtatccgcc accggtgagg atttaggctg tttcgtgttc aatgaaacga ctagaaagtt
     4741 ttcgttcttt cctaagatcg gcacgtacga cgaatgccac atcgacaaga gcgggaaata
     4801 cctcatgatc caggaaaata ccgacgggaa atacggtctg gagaatgtgt tcatcgatct
     4861 agaaacgggg aagcagagca tcgtgatgga tcagaacggt ggcttgggac atgccgatat
     4921 ggggttcggc tatgccctcg gaatggacaa ctggaattcc ttgccgggcg cagccatcac
     4981 cttcaatctt gccggcactg tgaaaggacc ctgcgtattt tacagcaaca attggaatat
     5041 gaatcccgtc aaccatgtct cccatttgaa tgccaaacct accatcccca tgtcccaaca
     5101 gttcgcgtgc ggcagtaacg ctgattacgg gtccgcacag aatgaagtcg tgtgctttcg
     5161 actggatgcc tcgcacgatc aacttatcgt tgcgcctgtg atgacagata tgaatgctcc
     5221 ggggggagga agcgagtacg cgaaatatcc caaaggtaat ctggatatca gcggcaggta
     5281 cttcatatgg accagcaata tgggcggaaa tcggttggat gccttcttag tgaaagttcc
     5341 ctctcatttg ttgaccggat cgagtgacat cgccgcaccg gcggctcctg tgaaccttcg
     5401 agtgacgacg cgtccctgaa gaatagctga cacgccttaa gccgccggac gctgtttttc
     5461 aaccgcatcg tccggcatca tctcagacca gtcatctgct cgcgccgcct cttcttccgg
     5521 ccgcctcgtg cgattccatg tcggccgtgc catcccgaag atgaatttca gtgagcccag
     5581 tgccaaggcc gcgttcaaga caacgaaata gcgcgctcca atgctcgctt tgccgacgag
     5641 aggcacggcg cgcaaccatc cggcacatag ggctgcccca tagaaaagcg cctgggagat
     5701 aaacagccat tgatagaacg gatagtgccg caggaatatg gctcccgcta gtaaagtcag
     5761 cagcatccat ggggccagga gccgcaaagc tttgtgcgac cacagagcca gtgaaaccac
     5821 gccccattgg ggcaacagca atcgccacgc gtgacggagc acatgggcaa ttccagcacc
     5881 ggatcgcacg cgccgacgaa attcgtccgc gactcgctcc ggtatgagtt gccacgcgcg
     5941 agcggcccca tactgtacga catggccgtg tgtgcgaagc cggaccagca acggttccaa
     6001 cagatcgtct gccaccgtat ccggaggtag cggacaatag tcctctcgac gaacggcgta
     6061 gatcgtgccg ctcgccccga gcccggcgcc aatttcggac tcgagtcctt ttaaaaagag
     6121 ttcgtaccgc caataccatc cgtccagatt gcgggagctg cgagacgacc gcatttcaag
     6181 ttctcccacc acgacggatg cggatggata gcgccaaaag ccggtgacta attgcctgaa
     6241 cgcatcggga taaaacaccg tggccgcgtc cgtgaagatc accatttcgc caccggcaag
     6301 cctgaccaga tcgttcaata cactggcttt tccccgccgg gtaggaaagg ccgccagctg
     6361 cacaccggca gaccggtaac tctcgaccac ctgacacgtg gcatccgtag acccgtccga
     6421 accgatgaga atttctatct gctcactcgg atagtcctgg tccaggagat tttggattcg
     6481 ccgtgcgatc accgcctcct cgttgtaggc ggaaatcaca accgtgagtg atggccagga
     6541 gttcgttgaa ccgcgttccg tctccagcgg gtctttgagt cgactgatca gccagagcaa
     6601 ggccgggtaa cccgcatagg tgtagacaag caagaccatt gcccaccacg tgagaatgat
     6661 catcgtcatc gaagcacctt ctgtcaaaaa tggaaggacc gctgtgccgg acaattctga
     6721 catggatgga ctcatggctt tcgtacttca aacacccgga tcgccgcgta ataggccagc
     6781 ccgggaaagc gggcaagcat ggcgcgatcc caccggcgta gcggcatcag gctcgcccgg
     6841 agaacgggaa ttcgttcagc ggcccgtgac catggcagct cgaaacttct cgatctgccc
     6901 gcaatgaacc ccgtcgagaa ctcggcgagc tctccaagcg tcaacgggcg ctcatacggc
     6961 gacacattgg cgggcaacgg acagggaacc atcttccgta atgcgcgcaa tgtcggcgag
     7021 ttccgcatcg gctcgctgaa gatggcccgg ccccccggtc gcagcacccg gtacacctcg
     7081 cgcgccgccc ggtcgagatc caaatggtgt aggatcgcca ttccgaagac gacatccacc
     7141 gaatcgtctg gacatggaac ctcataagcc gatgccacac tgaaccgtac gccactcgtg
     7201 acaccgttga cctgcatccg ccgacgattg atcgctatca attccggcga aatgtcgaga
     7261 gcatggacct cggcgcctag tcgggccaat ataatggtgt tctcacccca gccggaaccg
     7321 tactccaaca cctgttgtcc cgtaacatcg ccgagcaggt gaaaagcata ttccagatga
     7381 aatagcgtgt cggctggtgg attgacatac ctggccacat tgtcttcgga tgcaagcaag
     7441 gatgaagcat cagccgattc ggcttccgcc gcgctgcgcc tgatttccgc ctgttcccat
     7501 tcttgtaatg ccgtctgatg ttccataaca tcctttcggt tcattcactc ttggcagcct
     7561 gcgacttgtt gccccggtcc ttcgattgag gtttcgagca gacagcctcc atccggccgg
     7621 tatcgccgag agcggcgcag agggacttcg cgtctattcc agggacttcg gaggttggag
     7681 ggccgctcac ggcgctgctc gctgccacgc ggtaatctcc agccgcggca ttgatgaagg
     7741 gcaccgcctc cactgaggcc ggaaatgcgt tgtcttccgg ataccgctcg gcataggact
     7801 ctccgccgat caacatatta tggatcacga tcgctccggg aaaatgacgg tccagagtcg
     7861 gcttgcccac acctgtgccc tccccttcca tgccatattc gttgtgaggc gtgatgttgt
     7921 accgatatac gaacccttca tgcggatcgc cggcggccat gacagtatgc cccgtatgga
     7981 acccgacatt atgttcaatg acgacgtgct ttgtgcctct taataattga aacaatctgc
     8041 ccttcccgcc ccaacgcgga ccgttgatat cgtcaaaaat attgttcttg atcaggatgc
     8101 gttggccttg tccgctcggg ggatgaatgt catccgaacc cataatgttg acgccactcg
     8161 gactatgacg gacgatgttg ttggtaaagg tgacgtcttc gatgaccgcc caaggagaac
     8221 ggccttcctc attacggacc gtaaacgtga tcgcgaatcc atagccgggc cgctgccagt
     8281 tatattcaaa gatattgccg tccgccagga cgcggcgcgc gttcttcaac tcgaagagta
     8341 tcttgacctg ccagttgccg ccgtcgtgct ccggctctcc cggcctccat gacagcggct
     8401 tgaccacgtg gttatgacgg atttcgatat ccgatggcac gagatcacga atcgaaggat
     8461 ctccacctcc aaaaagaagg ttttcaccgg ctccttcgag caagttgttc tcgaccttga
     8521 acggtccgct cccgccccac ccgaccacgg cttgagaatc ctgtccctct tccctgaagc
     8581 ccgagacata tgagtcaacg atcgcgaggt gccggccgtt catcacaacg ccgcggcgcg
     8641 ctcctttctt gggatcgccg tgcacatagc agcggtcgag aatgatgtgg tgaggcaagt
     8701 cgtcgagaga ccgggcagta ttgtctccga gcaacacgag atcgcgaagc aggatgttgt
     8761 cctcaggctt gatctcaatg ccgatgaagc gatagtgatg cgcccgtggt tccgcgataa
     8821 tgacgccatg ctgattggac accagtgcag ccatcctccc agtttgcgag ggctggacgc
     8881 gtgttcccgg cgctggaaag tcttttccga tcccacccga acggatcgtg atccagcccg
     8941 aacccgcttt cttgtacggc aaggtaaagg gccctttgta ccgggcacca gccgtgagga
     9001 cgatctcgtc accaggctgt gcctcatcaa gcatggcctg gaagtcaccg ccggctggaa
     9061 cggtgagcgt gcgtcccggt gaagatttca cggtcgtatc gacgtactcg tgcggtaacg
     9121 ccgggcgtac gccggaagca gtcgcggcgc atccctcacc tatgggtatt gccagcatca
     9181 ccatcgctac catgaaagga cacatccagg aagatcggta ggtcatgccg cctccatgag
     9241 ccgctcttcg accaggctcg acatttcgcc gacccgcgct tcccagcctt cgctccgcat
     9301 catctccact cgaccacgat cccgctcgct ggtccgttcc ttcagcgcgg cttcgatttc
     9361 tcgcaaaaag ctttcccggt tggttgcaat gtgcacgacg ccttggtatc gcacaacttc
     9421 gggcaagggc gtggacacca ccgggaggcc ggcggcgagg tattcgcgta gtttgagggg
     9481 attgaccctc aacgtcagtt cgttcatacg aaacgggatc aggccgacat cgaaccctcg
     9541 gcaatagcct gggagcgccg aatagggtcg ctgtccgatg agatgcacgt tgggcaatcc
     9601 ctgcaccgca tgcagatcgg tctgcgcctt ccccaccagc gcgaaggacc attgcgggcg
     9661 agctcgtgcc aacgcgccga tcatgtcaag gtccacccaa tctgctaaca gaccgaaaaa
     9721 cccgatcacc ggcttctgga gggtggcgat ttctgacgcg acggccgtag cgggatccaa
     9781 ggctctactg aaatgagcca catcgacccc atgcggaata aaatgggtcc tcggattgat
     9841 ttcccggcgc tcagcacaca acttttcggc cgaagtgaac acaatgtcgg cacgtcgaat
     9901 gagctcccgt tccatgcgaa tgatgacatc tttcgggacg ccggaaaatt gggagtactc
     9961 atcgacgcaa tgatagatca ccatccgttc gtgcaggcgg cccaggagcc aattgacgtt
    10021 cggcaggaat gtccaaatga tgggccgtgg catgtctaat cggcggcaaa gatggcgaag
    10081 ccgtgccgcg agcacgatac gattcacccg ctccgccgcc ctgatacctg gcagcggcaa
    10141 cacgggaggg tccatgacat agatgttcgg ctcgacttga cggacgcccg agacgatgcg
    10201 ccgcagcttc gcgaccaatc gccggaggtc gcttgcggcc gccgtggggc ggcgcatgcc
    10261 gatcgagttc acccacagca cacggttccg tgctgaaaga atccgcatga tgtgcttctt
    10321 gctggtggga tcgccttccc aatcttggga gatgcaaata atcgattgcc cttcaatcat
    10381 gacgtttcac tcctttcaac acgcctgcga aaagcctcgg gagattaacg atttcccaca
    10441 cgtcatgccg gcaccgtttc ggcttccacc tgctcttcca atggctgagg catctcaccg
    10501 tcgatggcga tccgctgcca catttccagg ttcaccaaga gccagagacg atccgaatga
    10561 gcccgggtgc ctgacacgtg ctcgtcgacg atccggcgga ccgtctcggg atcaaagagg
    10621 tggcgatcca gtgcgcgtgt gctcaggacg tactcgtcga ggatcgtccg aaaaggcccg
    10681 cgcacccagc tcccgaaagg aaccggaaac cccatttttg atctgttcat cactgccggc
    10741 ggcaccatat tgcgtagcgc ctcacgaaga atgaccttcg tctgccaccg tctcatccgc
    10801 tgctcgccag ggatttgcat cacctgctcg acaagcagat ggtccaaata cggcacgcgg
    10861 ctttccaacg acgccgccat gctcatttga tcctgtttca tgagcaattc cacgaggtgc
    10921 gtcccgacgt cggcataact catccgctca agcaacgtgc cgcgatgctc gtgccagtag
    10981 cgcagacctt ccgcgtaggg atcaggaccc gtcgcggacc tctccttcag gagcgtgcgc
    11041 tgcagcgact cgggaaacac ggaaaaatta tcgaagaaca accctcgagg tccgggttca
    11101 taggccaatg ccgttcgcga ggcgtagcgt tgtatccgga ggggcagacg ccccaacgag
    11161 ccgctaatcc gtcgcctgag agcgcggggg acgctgctcc agtagaatcg gccgatgcga
    11221 tcgttcaggg cggtgacacg atagcggtaa tcgtatccga gaaacagctc gtcggcgccc
    11281 tcaccggtca ggaccacctt cacatgttgg ctggccaggc gggatacaaa gtataagggg
    11341 acgcttgatg ggaacgcgat aggctcgtcc tcgtgccaga caagcttagg caacgcctgc
    11401 ataaactgtt cgggagatac gagaacttcg tggtggtcag agcccacggc cttggccacc
    11461 agcctggcat actgaagctc gttggcctcc ccttcggcaa aaccgaccga aaacgttttc
    11521 agtccttgcg gcatcatagg agccatcaga gccgcaaggc cgctggaatc caaaccaccg
    11581 gaaagaaaga gacccagtgg cacatcactc atgagatggc tccgcacagc cgtctgcagt
    11641 cctgtccgca actgatcggc ctgctggctg agcggtgctc tgtcgtttac gctcagctgc
    11701 ggcggatgcc aatagcggcg tgttgtcgtc ccctgctccg ccgaccattc caagagatgc
    11761 ccgggcaata atttcaacac accgcgataa aacgtttccg ctcccgccac gtaccgatta
    11821 gccagaaatt ccggcaggat ggcgtggttg aacgacacgt tcggaatgcc ggaagccacg
    11881 atgcccttga tctctgacgc aaacagcaac tcgtgatcgt tgcaggcgta atacaacggc
    11941 ttgatgccga gtcgatcacg agcgagcagt aagcggc
//
LOCUS       DASTQY010000424         4811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_100546, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000424 DASTQY010000000
VERSION     DASTQY010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100546"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(528..1169)
                     /locus_tag="VFR82_13725"
     CDS             complement(528..1169)
                     /locus_tag="VFR82_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502708.1"
                     /translation="MPSSTPDVYIEEHNVVIAPGGLATRAWPVHWSAIWVGALASLAV
                     ALIFGLIGTAIGAHQVGKKVMNLHDLTLGGLIFSVGGAFFAFVVGGWVASSIAGLRRA
                     EDAALHGTITWLAALPLFLFLAALGAGSFFGGWYGGLAGTPIWATSSTVTADPNAAMV
                     ARNSAIGGVTALLIGLMGSLIGGWLASGEPMSLTYRRMKEDDDVSGRRRARAA"
     gene            1568..2740
                     /locus_tag="VFR82_13730"
     CDS             1568..2740
                     /locus_tag="VFR82_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEU4502709.1"
                     /translation="MLGLLLTAGSPASAELLALLNYESKPDQPVRREGIAIMDIDPAS
                     ADYGKILMEIPLPADLIAHHIFFNRDLTKAYITALGKPVLHVFDLTHFPYKLREIAVP
                     DCQVLEDLAVSEDNKKWYLTCMGSSNVIIGDAVNDKPLKTVSATDPHASILYPHGVAI
                     HSGIDRVIVTSTVKPDMSDAGDSVTVIEASTGKVLSTHKVSNKPDPAKAAPVEVMFSP
                     KSNPPVVHITNMLEATLWAGVWDPKKKEFSFTQIDDFGPRQQGMPLEMLYNAKGDRLF
                     VTTAKPGFVNLYDNTDPRRPKFLKTIPAAEGAHHSVLSPDERYLFVQNSLLNLEGMSD
                     GSITVIDLKDDKVLGSIDTLKKAGFNPNCIMLLPNHFQKGGLPNHPQKSRLRAAAQ"
     gene            complement(2985..3587)
                     /locus_tag="VFR82_13735"
     CDS             complement(2985..3587)
                     /locus_tag="VFR82_13735"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4502710.1"
                     /translation="MNGYVRFTCLLAVSCMVLWTAGCSKKSVQSDGRTSSAARPPVQE
                     PLPNLSLDDPSGTERLSSGIAVAKNDPATAKRQMDQARAEDAATAAAGLRDVFFGYDS
                     WTLSDDGRQALSRNAEWLRVHPAAQLKVEGHCDERGSSTYNFVLAEKRAKSILNYLSD
                     LGIKRERIVAVSYGKERPFCKDRSETCYQQNRRGHLVVRP"
     gene            4179..>4811
                     /locus_tag="VFR82_13740"
     CDS             4179..>4811
                     /locus_tag="VFR82_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502711.1"
                     /translation="MFHALRSFIQPIQTFFRETAGGFYHVVVIALSAGIALLLPSGAR
                     QFLSFWSRIEQDKLSLILIEMIVAVLLIGCFSYVRRSRRDRVLAKAAAGAGLISFFPR
                     HEPRAHQHIARLKEENTTGRTIMVIGSSGYSSFVDQVGDLSSVLDTCLGAKILLVNPY
                     SQEATTRIEAIDHPEYTLHAFREEVRQSIALLKRLRAMGKAVKLKLYADAP"
BASE COUNT         1058 a         1440 c         1312 g         1001 t
ORIGIN      
        1 ggtgaaggcg cgccgagcgc cctgaaacgt cgctctgaat cgaagcacat cgactagagt
       61 cgcaggagtg aacaagcggc catcgcttgg atggagtaac tgaagtgcct gcatatcgac
      121 gcgctgaatg cgatggtgat gggtgggagt tgcaacaaaa acgaggagag gctagcccct
      181 tcgccgaata gtgtcaatgg ttcagatcaa gttccttgta aaaaatagca cgtatttcct
      241 cacgagagaa aagatctgat acgtgagctt gtctgaaaaa gcatatgtct aagcgttttg
      301 gcttctcaat ggcctaatac ctattaatgg gtcattcctg tggattgtgg cgcgtgaagg
      361 agcacacccg tgggtgtgcc cccttccgct agacgatacc cgtcctatct gccgataccc
      421 acgacccaga gcagtacgat caagctcaat ggtagaccca atagccacat aatcacgtac
      481 gccatacgat acctcctctt actgagttcg tttacggatg cagccagtta tgcagctcgt
      541 gcacgccgac gtcctgacac gtcgtcgtcc tctttcatcc gacgataggt caacgacatc
      601 ggctcacccg acgccaacca gccgccgatg agactcccca tcaggccgat caacagcgcc
      661 gtgacgccgc cgatggcaga attgcgggcg accatcgcag cattgggatc cgcggtgacc
      721 gtcgatgagg tggcccagat cggggttccg gccagccccc cataccatcc gccgaagaaa
      781 ctccctgccc cgagcgccgc cagaaacagg aacagcggaa gcgccgcgag ccaggtgatg
      841 gtgccatgca aggcggcgtc ctcggcccga cgcaggcccg cgatcgatga tgcgacccag
      901 ccgccgacca caaacgcaaa gaacgctccg ccgacactga agatcaaacc gcccaacgtc
      961 aggtcgtgaa gattcatcac cttcttgccg acctggtgag cgccaatcgc ggtgccgatg
     1021 agcccgaaga tgagcgcaac ggcaagcgat gccaaggcgc cgacccagat ggcgctccaa
     1081 tgaaccggcc aggccctggt cgctaggccg cccggagcaa tgactacatt gtgctcctcg
     1141 atatagacat ccggagtgct gctaggcata actcctcctt gttaaagaca ctctcactat
     1201 tagagggttg caaagccagg accaaccaag cgtggagctg gtcgcggcga ttgtggcgta
     1261 acttcgcggg tctacagact agtgtttttc cctgagattc tcttcgcgct cgcagccatg
     1321 gaaaccctgt gctggcacaa gacaagtcgt ctgcgtcgcc tctgctagag ggacccctgg
     1381 ccggccaggg gtccctctag ccaagggtca ttggtagaaa gggtgaagac ggcggcttcc
     1441 ggttaaaaaa cggactgcca ctctctcatt gcttctcttt cgggaggagc gtaccgttat
     1501 cgttcttttc gccctcagac tacaaaaaag ggaggcccct atgacaccgt tccgattgct
     1561 cattccgatg cttggattgc tgctcaccgc gggatcgccg gcctcggcgg aactgctcgc
     1621 cctgctgaat tatgagagta aaccggacca accggtgcga cgcgaaggga tcgccatcat
     1681 ggacatcgat cccgcctcgg cggactacgg caagatcctc atggagattc cgctgccagc
     1741 tgatctcatc gcccaccaca tcttttttaa ccgcgatctg accaaggcct acatcaccgc
     1801 cttagggaag ccggtcctgc atgtgttcga cctcacccac ttcccctaca agctgcggga
     1861 gattgcggtg ccggactgcc aggtgctgga agatctcgcg gtgtcggagg acaacaagaa
     1921 gtggtatctg acctgtatgg gctcgagcaa cgtgatcatc ggcgatgccg tgaatgacaa
     1981 gccactcaag acggtgagcg cgacggatcc tcatgcttcg atcctctatc cgcacggcgt
     2041 ggccattcac agcggcatcg accgcgtgat cgttacgagc accgtgaaac cggatatgtc
     2101 ggacgcagga gactcggtga cggtcattga agcgagcacg ggcaaggtgc tgtcgacgca
     2161 caaggtgtcg aacaagcctg acccggccaa ggcggcgccg gtcgaagtca tgttctcgcc
     2221 gaagtccaat ccgccggtcg tgcacatcac caatatgctc gaagccacgc tctgggcggg
     2281 tgtgtgggat cccaagaaga aggagttttc cttcacgcag atcgacgact ttggcccgcg
     2341 gcagcaaggc atgccgttgg agatgctgta taacgcaaag ggcgaccgct tgttcgtcac
     2401 gaccgcgaag ccgggattcg tcaatctcta cgacaacacc gacccgcgcc gcccgaagtt
     2461 tttaaagacg atccccgcgg cggagggcgc gcatcacagc gtgctttcac ccgatgagcg
     2521 ctatctcttt gtgcagaata gcctgctcaa tctggaaggc atgagcgacg gatccatcac
     2581 ggtgatcgat ttaaaggatg acaaggtgtt aggcagcatc gacacgttga aaaaggcagg
     2641 ctttaacccc aactgcatca tgctgctgcc gaatcatttt cagaaaggcg ggctgccgaa
     2701 ccatcctcag aagagcaggc tgcgcgccgc cgctcagtag ggccggcagc gggagctgac
     2761 tgattcgggc gacgagaatt tctgccgcat ttcgcagcgg gaggaacagc gtgacttgaa
     2821 cgggcgaacg attgaccgag accgtcatga ccattctcac ccgcccagcc ctgcgcaatc
     2881 ttttcttacc caccgtcccc cgattgctct gcaatcgatt acccgggacg tgcgcttctc
     2941 cccacggcgg tctcagctca cttcaccata tcgtcattcg ccgtttaggg tctgacgacg
     3001 agatggcccc gacggttctg ctgataacag gtttcggaac gatccttaca gaatggacgc
     3061 tccttgccgt aggacacggc aacgatgcgc tcgcgtttaa tgcccaggtc cgacagatag
     3121 ttgagaatgg acttggcccg cttttctgcg agcacgaagt tataggtgga actcccgcgc
     3181 tcgtcgcagt gcccctccac tttgagctgg gcggccggat gcacgcgcag ccattcggca
     3241 ttgcggctga gcgcctgtcg tccgtcgtcg ctgagagtcc agctatcgta accgaaaaat
     3301 acatcgcgga ggccggcagc cgccgtggcc gcatcttcag cacgcgcttg gtccatctgc
     3361 cgcttggccg tcgccggatc attcttcgcc acggcaattc cagaactcag ccgctcggtg
     3421 ccggacggat cgtccaaaga caagttggga agcggttctt gaaccggagg acgcgcggca
     3481 cttgacgtgc gcccatccga ttgcaccgac tttttggaac atcccgcagt ccacaacacc
     3541 atacaactta ctgccagcag acaggtgaat cgcacataac cattcataca gcctccttgg
     3601 tgaacatcac gagacgcttg cccgcttgtg tcagcggctc ttcgtttcgc gcccgccgga
     3661 gtgtaccaag atgaacgcaa cagggcaata tgtaaggcga gtgaacgcac tacttgagtc
     3721 gctctagcga aagttaagat caggtaaaat ccgacctcgg cttgctcgtc tgccttatta
     3781 atgatcctcc caagctcgct tgttgttctt tccctagcct tgtggatggc ggggtgcacg
     3841 gccatgtcgc tgcggtcctt ctcacccccc aaaccttggc gcgccaagac gcgccttttc
     3901 ccaggcgccg attgtgagca ttgtccctgt cgctcagagg ataatcagtt gggagagttc
     3961 ctccaaggcc agtcatttcg gaattgactt aagtgcttgt gtcacttccc taaccatgct
     4021 aggttgacat tgatcgcgag acctacctgc atatcgacgc gatttatcga gatcgttctg
     4081 tctctggcga gcaccctccc gacacggacc tacgatcccc cgacatccga cagagcatga
     4141 tgttctgtct caacactaac tccggaagag gtgtagcctt gttccacgca ttgcggtcgt
     4201 tcatacagcc catacagaca tttttccgcg agacggcagg aggcttctat catgtcgtcg
     4261 tgatcgcttt aagcgcagga atcgccctcc ttcttccttc cggagccagg cagtttctca
     4321 gcttctggtc caggatagaa caggacaagc tttcactgat tctcattgag atgatcgtgg
     4381 ctgtgctgtt gatcggctgc tttagctatg tccggcgcag tcggcgggac cgtgttttgg
     4441 cgaaagccgc ggctggcgca gggctgatct ccttttttcc gagacacgaa cctcgcgccc
     4501 atcaacacat cgcacggctt aaggaagaga acacgacagg ccgcaccatc atggtgattg
     4561 gttccagcgg ctacagcagc ttcgtcgacc aagtcggcga tttgtcatct gttctcgaca
     4621 cctgccttgg tgcaaaaatt cttcttgtga atccgtatag tcaggaggcg actacccgca
     4681 ttgaagccat tgaccacccc gaatatactc tccatgcctt tcgggaagaa gtgcggcaaa
     4741 gcattgcact gctcaagcgg ctgcgagcca tgggtaaagc cgtcaaattg aaactgtacg
     4801 cagacgcacc a
//
LOCUS       DASTQY010000425         3909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_115592, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000425 DASTQY010000000
VERSION     DASTQY010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115592"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VFR82_13745"
     CDS             <1..1019
                     /locus_tag="VFR82_13745"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4502712.1"
                     /translation="FPTPLKQLLTIGRFEGLSPAERWKLLSWLEQLWEGTVQLATDLE
                     HRTAQQWLESLDVSRTSVQTIWNPLARWLTGSDIQSLSADAFVTALRPFFLSGAANSR
                     IWVAHPSRDQLLVEPVKGHLIKDGATLAFRTPAVRLEFHQERVTGVRVGDGSVLEAEW
                     YLAAVPPQQLTPLLPERWLTRYAYFQHIADLETSSSTVLQVRTATNLAKPRHILLSSG
                     PFHWMTCFSRAPDRRLLAVIRIQPGQPAPDLERQVSALLGSLNLLEPGRHLDELAQHE
                     IQHAVLPLGPGTKLRRPIHRSPIPNLLVAGAWTDTGWPANLESAIVSGERCADVIIGH
                     RLSV"
     gene            1099..1743
                     /locus_tag="VFR82_13750"
     CDS             1099..1743
                     /locus_tag="VFR82_13750"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU4502713.1"
                     /translation="MTLQELAKSLHNCQQCKLAKLGRTQVVFGVGNPRASIMFVGEAP
                     GFNEDQKGEPFVGAAGKLLNDLLASANLSRDQIYIANVIKCRPPNNRDPEPDEVETCK
                     PFLMQQIQMIRPKLVCTLGNWATQTLLERKVGITKVKAQAFYLKDFVIFPLLHPAAAL
                     HQGNLLDTLKEDFRKLKEFLDRHSQPPPDTEAAEPIAPALSLEPPNPAQMDLFG"
     gene            complement(2137..2604)
                     /locus_tag="VFR82_13755"
     CDS             complement(2137..2604)
                     /locus_tag="VFR82_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502714.1"
                     /translation="MPLDAEFGKKMLQLVTSRYDDRHWRKKIEKTLSLPQSGVGDPVQ
                     QQIFMYLKVGLKAYKSRRADPDSWIIGGYATKEVIERAKHQPKQVGADVTKDDVAFLG
                     NDPGTEVDEAWWDDMLVQWFDVPEAEGPAEGEVEEQPAESSSAASSERKSPKA"
     gene            complement(2659..3216)
                     /locus_tag="VFR82_13760"
     CDS             complement(2659..3216)
                     /locus_tag="VFR82_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEU4502715.1"
                     /translation="MRRACSMVLLAALLVPSAGMAADFRLSSPTLKDKGAIGNEHVFN
                     GFGCNGNNTSPELRWERAPKGTKSFAVTVYDPDAPTGSGWWHWVIFNIPPDTISLPAG
                     AGKPDSGGAPQGSIQSMTDFGQPGYGGPCPPPGDKPHRYIFTVYALKADQIPLQKEAS
                     GAMVGYYLNQNKLGKASFTSKYSRK"
     gene            complement(3300..3764)
                     /locus_tag="VFR82_13765"
     CDS             complement(3300..3764)
                     /locus_tag="VFR82_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEU4502716.1"
                     /translation="MHITSSAFAHQGAIPVLYTCEGRDVSPPIAWSELPPGTRSLVLI
                     VDDPDAPDPAAPKMTWVHWVLYNLPPTTNGLPEGVKTLPEGTREGVNDWKRTGYGGPC
                     PPVGRHRYFHKLYALDTMLPDLKQPAKSKLEDAMRGHILSQAQLVGTYQKGG"
BASE COUNT          774 a         1221 c         1061 g          853 t
ORIGIN      
        1 ttttccccac gccgctcaag caactcctca ccatcgggcg ttttgaggga ctctcgccgg
       61 ccgagcgttg gaaactactg tcctggttgg agcaactttg ggaaggcacg gttcagctcg
      121 ccactgacct ggaacatcgc acggctcaac agtggctcga atcgctggat gtcagccgaa
      181 cctctgtgca gactatttgg aatccgctcg cacgctggtt gacgggcagt gacattcaaa
      241 gcctgtccgc agacgccttc gtgaccgctc tgagaccgtt ctttctttcc ggcgcggcca
      301 acagccgaat ttgggtggca catccatccc gcgaccaact gctcgtggaa ccggtcaaag
      361 ggcacctgat caaagacggc gcgacattgg cctttcgcac accggctgta cgacttgagt
      421 ttcaccaaga gcgggtgacc ggcgtacgtg tgggagacgg aagcgtcctc gaagccgagt
      481 ggtacctcgc cgcagtccct ccgcagcagt taacgcctct gctgcccgag cgttggctta
      541 cgcgttatgc ctactttcag cacatcgccg atcttgaaac gagctcctcc actgttcttc
      601 aggtacgcac cgcgacgaac ctggcgaagc cacggcatat ccttcttagc tccggaccct
      661 tccactggat gacgtgcttt tcaagagcac cggaccgacg tctgctcgct gttataagga
      721 tccagcccgg ccaaccagcg cctgatcttg aacggcaggt ttcggcgctt cttggttcgt
      781 tgaatctcct ggagccaggc cgtcatctcg acgaattggc gcagcacgag attcagcatg
      841 ccgtcctgcc ccttgggccg gggacgaagc tccgtcgacc gatacatcgg agcccgattc
      901 ccaatctgct cgtggccggg gcctggaccg acacgggctg gccggccaat ctggaaagcg
      961 cgattgtcag cggcgagcgt tgcgccgatg tcatcatcgg ccaccgtctc tctgtttaag
     1021 tgcgagcgat tttctgcgtt gacaactttt tgccccgctc ctaagatgcc tcgcgtcggt
     1081 ccatcgtcac acctcaccat gacactgcaa gaactcgcga aatcgcttca caactgtcag
     1141 cagtgcaagc tcgccaaact ggggcggaca caggtggtgt ttggggtggg taatccccgg
     1201 gccagcatca tgtttgtcgg cgaagcaccc gggttcaacg aggatcagaa gggcgagcct
     1261 ttcgtcggcg cggccggcaa attattgaat gatctgctgg catccgccaa tctctcgcgc
     1321 gatcagatct atatcgcgaa cgtgatcaag tgccggccgc cgaacaaccg cgaccctgaa
     1381 cctgatgaag tcgagacctg taagccgttt ttgatgcagc agattcagat gatccgacca
     1441 aagctggtgt gcacgctcgg taactgggcc acacaaacct tgctcgaacg aaaggtcgga
     1501 attacgaagg tgaaggccca ggccttctat ttaaaggact ttgtcatctt tcccctgctg
     1561 cacccggctg ctgcactcca tcaaggcaac ctgctcgata cgttgaagga agatttcaga
     1621 aaactgaagg aattcctaga ccggcattcc caaccgccgc ctgacacgga agctgccgag
     1681 ccgattgcgc ccgccctcag cctcgaaccg cccaaccccg cccaaatgga tctgttcgga
     1741 tagttgagtg tcgcggcgat ggtgtcccgg actgctccag cgcttcggct cgaccacgct
     1801 aagcttctca tctgctcgac gccgaaactc gttcccttct tagcttcgct gcaagggaac
     1861 ttcgaacatc ggcatcgcca gccaagggct ggtcgcatct gactacccag tgcggtgccc
     1921 gagccttcgc gcaagtcgca gacgggcatc catcgccgca ccctattatc ctcactcggc
     1981 aagaagagag aagtgactgc ccgctgcggc ctcgctggac gcccattgtg atcagcctgc
     2041 gttcagcgat actgcggctc ggccgacggg ttgaaccgca tagttacggc aagccagcaa
     2101 gcgctgccga aaggggaagc aacgcaggca acacaattag gccttcggtg attttctttc
     2161 cgacgatgcg gcagagctcg attcggcggg ttgttcctcg acttctcctt cagcaggccc
     2221 ctccgcttcc ggcacgtcga accactgcac caacatgtca tcccaccagg cttcgtccac
     2281 ttccgtaccg ggatcgttgc caagaaaagc cacgtcatcc ttggtgacat cggctccgac
     2341 ctgtttcggc tggtgcttgg cgcgctcgat tacttccttt gtggcatacc cgccgatgat
     2401 ccaggaatcc ggatcggccc tgcgggactt gtacgctttc agtccgacct tcaagtacat
     2461 gaagatttgt tgttgaaccg gatcgccgac gcctgattgc ggcaagctga gtgtcttttc
     2521 gatcttctta cgccagtgcc ggtcatcgta acgcgacgtc acgagctgca gcattttctt
     2581 tccgaattcg gcatcaagcg gcattgactt cctccatcag cggcgtctcc tcgtccctca
     2641 gctgctcatt tgtcgtagtt actttcggct gtacttgctc gtgaacgagg cctttccgag
     2701 cttattctga ttgaggtaat agcccaccat cgcaccggag gcctctttct gcagaggaat
     2761 ttgatctgct ttgagcgcat acaccgtaaa gatataccga tgcggtttat caccgggcgg
     2821 tggacaggga ccgccgtatc ccggctgacc gaaatcggtc atggattgga tgctgccttg
     2881 cggcgctcct ccgctatccg gcttcccggc accggccggc agggatatcg tatccggagg
     2941 gatattgaaa atcacccaat gccaccaccc gcttccggtc ggcgcgtccg gatcgtagac
     3001 cgtcacggcg aaactcttcg ttcctttcgg tgcccgctcc catcgtaatt ctggcgaggt
     3061 gttgttgccg ttgcatccga atccgttaaa gacatgttca ttaccgatgg cccctttgtc
     3121 tttcagcgtg gggctgctga gccgaaagtc ggcggccatg cccgcgctcg gaaccagcag
     3181 cgctgccagg agaaccatac tacatgcacg tcgcataacc actcctctct gtggatctcg
     3241 tgacatcgca gaccgcgcgg atggcttgga taactgcccg cagtacgatc aaacgaactt
     3301 catccgccct tctggtaggt cccgactaac tgggcctggc ttaaaatgtg gcctctcatc
     3361 gcgtcctcga gcttgctctt tgccggctgc ttcaggtcgg gcagcatggt atccaatgca
     3421 tagagcttat ggaagtaccg gtgacgccca accggcgggc aaggaccgcc ataaccggtt
     3481 cgcttccaat cattgacccc ttctctggtt ccttccggca atgtctttac gccttccggc
     3541 aagccgttgg tcgtcggcgg caaattgtac agcacccaat gtacccaggt cattttcggt
     3601 gcggcaggat cgggcgcgtc ggggtcatcc acaatcaaca ccaaacttct cgtaccaggc
     3661 ggcaactccg accaggcgat cggcggtgag acgtcccggc cttcgcacgt atacaacacc
     3721 ggaattgcac cctgatgggc aaaggcgctc gaggtaatat gcatgttgcc ctccccgcgg
     3781 gctgaagcgg ccatggcagt aagaacaagg gcgagcgggg ctgccagaaa ggagtaacga
     3841 actcctgcag gcagacgtcc gaggggtcgg aagctcttca ctgtgcttgc tcagttaccc
     3901 gcgaccatc
//
LOCUS       DASTQY010000426         2440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_733836, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000426 DASTQY010000000
VERSION     DASTQY010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733836"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VFR82_13770"
     CDS             <1..654
                     /locus_tag="VFR82_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502717.1"
                     /translation="FHILTLAGLLLMPAEGWNGVTDKAGVPPRLSLQELEGMLPPGTR
                     LMVHPAAIEKFLAELDGAPPDWETLHGRGHQDPGHDDRLFAFNRERDARRGGHEALGQ
                     AVAFLWTGELSRYDPGSDGFPIALGPKLVKTTWGMVRFKPEDVPANLNVTTDVIRREQ
                     WRRQIERGHPVEIDVVMSGKLIPDESIVYDFSHDEEGLGLIMPFVRVERVDFVLINP"
     gene            complement(656..1567)
                     /locus_tag="VFR82_13775"
     CDS             complement(656..1567)
                     /locus_tag="VFR82_13775"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU4502718.1"
                     /translation="MPVVSESPDVRHSAGWFILCCVTLMMSSEWSFGFSDSTMVFIPA
                     GEFTMGTPAGTDGLADEHPQRFVFLSSFYIDRYELSNAKYFAFVSATHHRIPENANPA
                     STLWINGAPMRGIEQHPVVNVSWTDAEAYCRWAGKRLPTEAEWEKAARGTDGRRYPWG
                     NQWDIALANSASFWAGRTIEFDSGADWDAFWVKGEGAQIAKSKGLNGEVLTLPVGSFP
                     TNASAYGLLDMAGNVAEWVQDWYDPNYYKYAPLDNPVGPDRGAIKSMRGGSWLKPAVS
                     LRTSDRDWGTMDSRPSGTGFRCATDAF"
     gene            complement(1542..2309)
                     /locus_tag="VFR82_13780"
     CDS             complement(1542..2309)
                     /locus_tag="VFR82_13780"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEU4502719.1"
                     /translation="MRKATRMTRPQTNSRFQEELLRIMDRKHHWAWPSFADGTVSVEQ
                     LTRHFQQEYAVYVRDFPVMLARIHGHNPPAAVRRMLAENIYEEDTGGLSLGKSHPELF
                     LQMMAGLGLPAKSFERITLLPAARRYRAWLDRASYHRQWIVGAAALTVFVEGSVKDRK
                     ELDEPSKPKTAEEIESIVQQHPLVRHYGLSADSMDLIRSHQMVEAGHRHDAYKMVVSY
                     ATTRAQQQAVLSCLKTCLRLWSAYRDGVARACRLRKP"
BASE COUNT          487 a          693 c          729 g          531 t
ORIGIN      
        1 tttcacatcc tgaccttggc ggggttattg ctcatgccgg ctgaaggatg gaacggtgtg
       61 accgacaaag ccggcgtgcc tccgcgcctc tccctccaag aacttgaggg catgcttcct
      121 ccaggcaccc ggctcatggt ccatcccgct gccattgaga aatttttggc agaattggat
      181 ggcgcaccgc cggactggga aacgctgcac ggtcgcggac accaggaccc aggtcatgat
      241 gaccggctct ttgcgttcaa cagggagcgg gatgcgcgac gtggaggcca cgaggcgctg
      301 ggacaggcgg tggccttcct atggacgggc gaactttccc gttatgatcc ggggagcgat
      361 ggttttccca tcgcccttgg cccgaagctc gtcaaaacta cctgggggat ggtccgtttc
      421 aagcctgaag acgtgccagc gaatctgaac gtcacgaccg acgtgatccg gcgtgagcaa
      481 tggcggcgcc aaatcgagcg ggggcacccg gtcgagatcg atgtcgtgat gagcgggaag
      541 ctcatacctg acgaatcaat tgtttatgat ttttctcacg acgaagaggg cttggggctg
      601 atcatgccct tcgtgcgagt cgagcgggtg gattttgtct tgataaatcc ttaagtcaga
      661 aggcgtctgt tgcgcagcgg aaacccgttc cactcgggcg gctgtccatg gtgccccagt
      721 cgcgatcgct tgtgcgcaga cttaccgctg gcttaagcca cgagcctccg cgcatggatt
      781 tgatggcgcc acgatcagga ccaacggggt tatccaacgg ggcgtatttg taatagttgg
      841 gatcgtacca atcctgcacc cattcggcga cattgccagc catgtcgagg agaccgtatg
      901 cgctggcgtt ggtgggaaaa ctcccgaccg gcagggtcag cacttctccg ttcaatcctt
      961 tgctcttcgc gatctgggcg ccttcaccct tgacccagaa ggcgtcccaa tccgctccgc
     1021 tgtcaaactc gattgtgcgt ccggcccaaa agctggcgct gttcgccaac gcgatgtccc
     1081 actgattgcc ccagggatat cgacggccat ccgttcctct cgcggctttt tcccattctg
     1141 cttcagtcgg aaggcgcttg ccggcccatc gacaataggc ttccgcatca gtccagctga
     1201 cattcaccac cggatgctgt tcgatcccgc gcatcggcgc cccgttaatc cataaggttg
     1261 atgctggatt ggcgttttcg ggtattcggt gatgtgtcgc ggagacgaat gcgaaatatt
     1321 tggcattcga gagttcataa cgatctatgt aaaaggagga gagaaacaca aaacgctgtg
     1381 ggtgctcatc ggcgagtcca tctgtgccgg ccggcgttcc catcgtaaat tcaccggcgg
     1441 gtatgaatac catggtgcta tctgagaagc cgaagctcca ttcggagctc atcatgaggg
     1501 tgacgcagca cagaataaac cagccggcgc tgtgccgcac gtcagggctt tcggagacga
     1561 caggcacgag cgaccccgtc gcggtaggca ctccacaatc tcaggcaggt cttcagacaa
     1621 ctcaggaccg cttgttgttg cgcacgcgtc gtcgcgtagc tcacgaccat cttgtaggcg
     1681 tcatgtcgat gcccggcctc gaccatctga tgagaacgga tgagatccat ggaatcagcc
     1741 gagaggccat aatggcggac cagcgggtgt tgttggacga tcgactcaat ttcttccgcg
     1801 gtcttgggtt tggaaggctc gtccaactcc ttccggtcct tcacgctgcc ttccacaaag
     1861 acggtcagcg cggcggcacc gacgatccac tgccgatgat aggaagcacg gtcgagccag
     1921 gctcgatatc gacgtgcggc gggaaggagc gtgatccgct caaaggattt ggcggggagg
     1981 cccaatccag ccatcatctg aagaaacagt tccggatggg atttcccgag tgaaagacct
     2041 ccggtgtctt cttcgtaaat gttctcagcc agcatccgcc ggaccgcggc cggagggttg
     2101 tggccatgaa tccgtgcgag catgaccgga aagtcgcgca cgtagaccgc atactcttgc
     2161 tgaaaatggc gggtcaattg ctcgactgag actgttccgt cggcgaatga tggccaggcc
     2221 cagtggtgct tgcggtccat gattcgcagg agctcttctt gaaagcggct gttggtctgc
     2281 ggtcgggtca tgcgggttgc cttcctcatc agggcctcgt tacaattcgc cggatggagg
     2341 acatgtgaaa caggtgaccg ttcaaaaccc cgatgaaatc ctgtcgctgc tggccgaggt
     2401 cgcgttacgc ggatctggtt tcgtcaccga atgccttctc
//
LOCUS       DASTQY010000427         4268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1173482, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000427 DASTQY010000000
VERSION     DASTQY010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173482"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..1371
                     /locus_tag="VFR82_13785"
     CDS             298..1371
                     /locus_tag="VFR82_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEU4502720.1"
                     /translation="MAIQDESEPAPDVSPDTRRNDLTDWATWPDERLLDLRLCDLDVK
                     IAGSNLEDRIAQLYQELDDRGLRFHPHFWLSDEWFCPDDVPGIAIPFYLAHPRLAKLE
                     LAQMLEVEGGDPDSCMRILRHEAGHAIENAFKLRRRRHRQQLFGNSSEPYPDDYSPKP
                     YSKSFVLHLDSWYAQSHPDEDFAETFAVWLTPNSLWETRYAGWPALKKLQYVDQLMKT
                     LVGQPPLHVSTEEIDPLRRLRTTLRRHYKKKRRHYGVDHPDFNARDLQRFFSEAAEHA
                     DNMTAIRFLMKIRKPVRRLVAGWTGVYQYTIDQVFEDLIDHCRPLNLRLAVPEEQAKQ
                     EFTVFLTVQAMNYVHTGGRRVAL"
     gene            1368..2393
                     /locus_tag="VFR82_13790"
     CDS             1368..2393
                     /locus_tag="VFR82_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU4502721.1"
                     /translation="MKPLKVLVLMHKHLVPPQNTSGVDTQKAEWKTEFDVVTTLRTLG
                     HEAEPLGVEQDLGIIRSTIDTWKPHIVFNLLEAFHNVSLFEPNVVSYLELLRIPYTGC
                     NPRGLLIAKDKGLSKKLMAYHRIPVPEFAIFHRGLKIRLPKRLAFPLIVKSLTEEASL
                     GISQASVVEDEQKLRERVGFIHESVGTDALVEEFIEGRELYVGVMGNQRLRVFPVWEM
                     QFSKMPDNVHRIATERVKWSNKYQEKYGIQTAELKDVSDSVIEHIQHLSKRVYRSLDL
                     SGYARIDLRMDTNGKLYVLEANPNPQIAEVEDFAESAKQSGVPYKQLLQQIITLGLQW
                     RPEGNGG"
     gene            2600..2992
                     /locus_tag="VFR82_13795"
     CDS             2600..2992
                     /locus_tag="VFR82_13795"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4502722.1"
                     /translation="MKQVFLALSLMLTCLVTVVACDDGKPAGTATTAVGADTALMAPP
                     GSPGRAINDEGVGHYKQGHWDVASEGFKKAIKADSNLAEAHYNLALSLDKMGKHEDAT
                     GSFKKAAELAPTNPAIKDSPILKKHLGM"
     gene            3303..>4268
                     /locus_tag="VFR82_13800"
     CDS             3303..>4268
                     /locus_tag="VFR82_13800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4502723.1"
                     /translation="MSPLKRMDEIKPESLSALSESQAHYRTLFNLAPIAVYSCDASGV
                     IREYNNRAAELWGRKPAPGDTDERFCGSFKMYRPDGSFMPHEQCPMGDVLSGKASGTH
                     DAEVHIERPDGSRVIVIVNIAPLMNDPGGEITGAINCFYDITERKQAEQTLRASEERY
                     RHLYESIDEGCCVIQVLFDANNRAVDYQFLEVNPAFEKQTGIAHAQGKNMRAIAFHHE
                     EHWFEMFGEIALTGQSRRFEFPAAELHRWYEGYAYRFGEPDERKVGILFNDITERKRV
                     EAQLRQLNDRLEARVTERTEALTESQKRLRVLAAALNVTEQRERQR"
BASE COUNT          993 a         1205 c         1199 g          871 t
ORIGIN      
        1 ccgcaggctg ttcagtcggc ctcacctacg gcggacccta cgctcccccc gggcgcacgt
       61 caacaaggga ggggctgact atttttcatg aatcgacaaa cgccttggca ttctgaacag
      121 cccatgctca tatgcctatg agcgcataca tgacccaaat atgaaggata ggtccagccg
      181 ttggtttttt cctccgaacg actgaactgg caaggcccac cggtggggat cgactggact
      241 atcggtcaaa acgtacaatg tcccggcttt gggactcatg ctgtgttgac ctgaacgatg
      301 gccatacaag acgagagcga gccggcacca gatgtgtccc ccgataccag acgaaacgat
      361 ctcaccgatt gggcgacgtg gccggatgag aggttgctgg atcttcggct gtgcgacctt
      421 gatgtcaaga ttgcgggtag caatcttgag gaccgcatcg cgcagctata ccaagaactt
      481 gatgaccgcg gccttcgctt tcatccccat ttttggctgt cagatgaatg gttttgtcct
      541 gatgatgtgc ccggcatcgc gatcccgttc tacctcgccc acccccgcct cgccaagctc
      601 gaactcgctc aaatgttaga agtagaagga ggcgatccgg attcatgcat gcgcatcctg
      661 cggcatgagg ccggccatgc gatcgagaat gccttcaagc tgcgacgccg acggcatcga
      721 cagcagcttt tcggaaatag ctccgagccg tatccggatg attattctcc aaagccctat
      781 agcaagagtt ttgtgcttca tctcgactcg tggtacgccc aaagccatcc agatgaggac
      841 ttcgccgaaa ccttcgccgt ctggctgacc ccgaattctc tctgggagac acgctacgcc
      901 ggatggccgg cgctcaagaa acttcaatac gtcgatcagc tcatgaagac gctggttggt
      961 cagcctccgc ttcacgtctc cactgaagaa attgatcctc tccggcggct tcgcactacc
     1021 ctgcgccggc attacaaaaa gaaacgccgg cattacggtg tcgaccatcc ggacttcaac
     1081 gcccgtgacc tgcagcggtt cttctcggag gcggcagagc atgcggacaa catgacggcc
     1141 atccgttttc ttatgaagat tcggaagccg gttcgccgtc tcgtcgccgg ctggacgggt
     1201 gtgtatcaat acacgatcga tcaagtcttc gaagatctga tcgatcactg ccgtccgctc
     1261 aacctgcgcc tggcagtgcc ggaagagcag gctaaacaag aatttaccgt cttcctgaca
     1321 gtccaagcga tgaactacgt ccacaccggc ggccggcggg tggcgctatg aaaccgctca
     1381 aagtcctggt gctcatgcac aagcatttgg taccgcctca gaatacatcc ggtgtcgata
     1441 ctcagaaggc cgaatggaag acggaatttg acgtcgtgac gacgctgcgg acgcttggcc
     1501 atgaggcaga gccgttggga gtggaacagg atctcggcat catccgcagc accatcgata
     1561 cctggaagcc acatattgtc ttcaatctgc tggaggcgtt tcacaatgtg agcctgttcg
     1621 aacccaacgt ggtgagctac ctcgaattac tacgcattcc ctacaccggc tgtaatccac
     1681 gtggcctgct gattgccaaa gacaagggac tatccaagaa gctcatggca taccatcgca
     1741 tcccggtgcc ggagttcgcc atctttcatc gaggactgaa gatccgcctg ccgaagcgcc
     1801 tcgccttccc cctgatcgtc aaatcgctga ccgaggaagc ctcgctcggt atctcgcagg
     1861 catccgtcgt agaggatgaa cagaagttac gcgaacgggt cggattcatt catgagagcg
     1921 tcggcacgga tgcactcgtc gaagagttca tcgaaggccg cgagctctac gtcggcgtga
     1981 tgggcaatca gcgactgcgg gtctttccag tgtgggagat gcaatttagc aagatgccgg
     2041 acaacgtaca tcggatcgcc acggagcggg tcaagtggag caacaagtat caggagaaat
     2101 acggcatcca gaccgccgaa ctgaaagacg tgtccgacag cgtcatcgag cacatccaac
     2161 atctctctaa acgagtctat cgttccttgg acctgagcgg gtatgcgcgg atcgacctac
     2221 ggatggatac aaacgggaag ctctatgtgc tggaagcgaa tcccaatccc caaatcgcgg
     2281 aggtcgagga tttcgccgaa tccgccaaac agtccggcgt gccctacaag cagctgctcc
     2341 agcaaatcat caccttgggc cttcaatggc ggccggaagg caatggggga tgaggacgcg
     2401 ccagttcctg acaagccccg accgactcac gcatctacgg tgacaggaca aggaaacggt
     2461 gaaagagaca tggacgactg gaccacgact ggttgatccc tcctgcgcgg cgccagtgag
     2521 cactattgat cagaccggac atcccgaatg agacatgatc ggaacaagtt tctgatccat
     2581 gtcacctagg aggttcaacg tgaaacaggt ctttctcgca ctgagtctga tgcttacgtg
     2641 tctcgtcacc gtggtcgcgt gtgatgacgg caagcctgcc ggaacggcaa ccacggcagt
     2701 gggtgccgac acagccctca tggctccccc cggttcacct ggtcgtgcga tcaatgacga
     2761 gggcgtcggc cactacaagc agggacactg ggacgtggcg tcagaggggt tcaaaaaagc
     2821 catcaaggcc gattcgaacc ttgcagaggc ccactataac ttggctctct cactcgacaa
     2881 aatggggaag cacgaggacg ccacggggtc cttcaagaag gcggcggaac tggcccccac
     2941 gaatcccgcc atcaaggatt caccgattct caagaagcac cttggaatgt aacgagcgga
     3001 gtctgcctcg cctggaatcc gctgtttggg aggtcgatcg tccgcgcgga tgggagaggt
     3061 gcgagcgacg tgcgctccgg cggtcgctta tccggaatac aagaagaggt tcacgacttt
     3121 tcgcacccga tcctaactgt tgatgtcggt gtacgtctga cctccgcccg ggttcctcaa
     3181 cccattgaga atgcacagga ttggcctttt ttatccgtgt gagcaatttt gatcagacac
     3241 ccttcttgcc tcttcttcat agtcggcccc tcatgatgca gttcatgcac gatggagatc
     3301 tcatgagccc tctgaagcga atggacgaga ttaaaccaga gagcctttca gccttgagtg
     3361 aaagtcaggc gcactatcga accttgttca acctggctcc cattgccgtc tattcctgtg
     3421 atgcgtcggg agtcatccgg gaatacaaca accgcgcagc ggaactctgg ggccgcaaac
     3481 cagcgccggg ggataccgat gagcggttct gcggttcctt caagatgtac cgcccagacg
     3541 gcagttttat gccacacgag caatgtccca tgggcgacgt gttgagtgga aaagcatccg
     3601 ggacgcatga cgcggaagtg catatcgagc ggcccgatgg ctcgcgagtg atcgtcatcg
     3661 taaatattgc tccgctgatg aacgatccgg ggggggaaat cacgggagcg atcaattgct
     3721 tctacgatat caccgagcgc aagcaggccg aacaaacgtt acgtgcgtcc gaggagcgct
     3781 accgacatct ctacgagtcc attgacgaag ggtgctgcgt gatccaagtg ctgtttgatg
     3841 cgaataatag ggcggttgac tatcaatttc tcgaagtcaa tccggcattt gaaaagcaga
     3901 ccggcatcgc acatgcacag ggcaagaaca tgcgcgccat tgccttccac cacgaagaac
     3961 actggtttga aatgttcggc gagattgcct tgacggggca atcgaggcgg ttcgaatttc
     4021 ccgccgccga actgcatcgc tggtatgaag gttatgccta ccgtttcggt gaacctgacg
     4081 agcgaaaggt cggtatcctg ttcaatgata ttacggagcg caaacgggtg gaagcacagc
     4141 tgcgtcaatt aaatgacagg ttggaggcgc gggtcaccga gcggacggag gcattgaccg
     4201 aatcgcagaa gcggttgcgc gtcttggccg cggcactgaa cgtgaccgaa cagcgtgagc
     4261 gccagcgg
//
LOCUS       DASTQY010000428         3659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1170917, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000428 DASTQY010000000
VERSION     DASTQY010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170917"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFR82_13805"
     CDS             <1..269
                     /locus_tag="VFR82_13805"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HEU4502724.1"
                     /translation="IDPACGCTVCARYSRAYLHHLYNVKEMLASRLNTLHNLWFFSDL
                     MRRMRVAIAQGTFPSFAESFYHQQSQEPAGRIASAKTGSDQSSC"
     gene            324..659
                     /gene="yajC"
                     /locus_tag="VFR82_13810"
     CDS             324..659
                     /gene="yajC"
                     /locus_tag="VFR82_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU4502725.1"
                     /translation="MMLMESVAWAQGTGGSGSGSGTILSLVPFILIFVIFYFMLILPQ
                     QKKQKQQKAMMESLKKGDKVITSSGIWGTVTNLGKDTVTLQISDNTKIKMQREFIARV
                     RSDEDDKDT"
     gene            687..2330
                     /gene="secD"
                     /locus_tag="VFR82_13815"
     CDS             687..2330
                     /gene="secD"
                     /locus_tag="VFR82_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEU4502726.1"
                     /translation="MKKVRARLLLLLLVLLISIVCFLPSYPNLYQALPTWMKSILPNK
                     GIALGLDLQGGIHMVLEVDEDRAVEIAVDRSVVSIQDLLVEKKIPAESVKRTSPTRIV
                     IQFQNADLKAQIQKLLDEFPTFSEVESAGSGNTIAWELRETESKRIKDSAINQALETI
                     RNRIDQFGVAEPLVQRQGMKQIVVQLPGVRDPKRAKDLIKETALLEFKMLDEDNQMKL
                     DLPSRIPKDREAEVVKQAEAKLPEGSQVLFERAVERDTGREYRIPYLVKKRVMLTGDV
                     LSDARVSIGQFNDPYVSITFDAKGGREFERITGENVKKRMAIVLDNTIYSAPVIQERI
                     SGGRAQITGTFSTQEANDLAIVLRAGALPAPLKIIQDLTVGPSLGKDSIEKGVRATLI
                     AGATVIVFMIVYYRLSGVIANFALVLNLICLMGALSALNATLTLPGIAGIVLTIGMGV
                     DSNVLIFERIREELRAGKAVRLAVDGGYDKALLTIVDSHVTTLITGVALFLFGTGPIK
                     GFAVTLCLGIAINLFTALVGTKVIFDIINQRHKVEQLSI"
     gene            2346..3263
                     /gene="secF"
                     /locus_tag="VFR82_13820"
     CDS             2346..3263
                     /gene="secF"
                     /locus_tag="VFR82_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEU4502727.1"
                     /translation="MLEILGKTNFDFMGKRRYAFLFSGIMVVLGIIGLIQIARGAANL
                     GIDFAGGTAVQLKFDQPIGIEEARKALETNGLGNAELQEFGQENKLLIRVKASTTIEE
                     KTAEKVVSIFTKEFPGNRFVVESSTEIGPTIGKKLQSDALVAIVISFAGIILYIAARF
                     ELRFGVAAALATFHDVLAVLGAFFLLDKEITLLVITALLTLAGYSLTDTVVVFDRIRE
                     NLRLRRREHEVTMINNAINQVLSRTIVTSLTVVLVLIPLTLGGGEVLHDFSLALLWGV
                     IFGTYSSIFVASPLLLLWPGGAGRLLKRA"
BASE COUNT          795 a          956 c         1125 g          783 t
ORIGIN      
        1 ctatcgatcc ggcctgcggc tgcaccgtgt gcgcgcgcta ttctcgcgcg tacctgcatc
       61 atctgtacaa cgtgaaggag atgctcgctt cccgactcaa taccctccat aacttatggt
      121 ttttttccga cttgatgcgg cggatgcgcg tggcgatcgc acagggtacc ttcccgtcgt
      181 ttgccgaatc cttttatcat cagcagagcc aagagccggc cggcaggatc gcatcggcaa
      241 agacggggag cgatcagtcg agctgctgaa tgttgaggcg gtgtgagttg gataagacca
      301 gcgcctggta agagaggaac atcatgatgc tgatggaatc ggtcgcgtgg gcacagggca
      361 cgggtggcag cgggtcgggg tcggggacga tcctctcgct ggtgcccttt atcttgatct
      421 tcgtgatctt ctatttcatg ttgattctgc cgcagcagaa aaagcagaag cagcaaaaag
      481 ccatgatgga gtcgctgaag aaaggcgaca aagtgatcac gtcgtctggg atctggggca
      541 cagtcacgaa tttgggaaaa gacacggtga cgctgcagat ctccgataat acgaaaatca
      601 aaatgcaacg ggaattcatt gcgcgggtac gctctgacga agacgacaag gacacctagc
      661 cgacggacgg agggattgga ctcgatatga aaaaagtacg tgcgaggctc ttgttattgc
      721 tgctggtcct gctgatatca atcgtctgct tcttaccgtc ctatcccaac ctctatcagg
      781 ccttgccgac gtggatgaaa tccatcttgc ccaacaaggg catcgcgctc gggctggatt
      841 tgcaaggcgg catccacatg gtcctggaag tcgatgaaga ccgggccgtg gaaatagcgg
      901 tggatcgctc ggtggtgtcg atacaggatt tgctggtcga gaagaaaatc ccggccgaat
      961 ccgtgaaacg gacgtcgccg acgagaatcg tcattcaatt ccagaacgcc gacttgaagg
     1021 cgcagatcca gaagctgctc gatgagtttc ccaccttcag cgaggtcgaa tccgccggct
     1081 ctgggaatac gatcgcctgg gagctccgag agacggaaag caagcgcatc aaagactccg
     1141 cgatcaacca ggccttggaa acgattcgca atcgtatcga tcaattcggt gtcgcagaac
     1201 cccttgtcca gcggcagggc atgaagcaaa tcgtcgtgca gttgccgggg gttcgggatc
     1261 ccaagcgggc gaaggatctg atcaaggaaa ccgccctgct cgaattcaaa atgctcgacg
     1321 aggacaatca gatgaagctc gatctgcctt cccgcattcc aaaggacagg gaagcggagg
     1381 tcgtgaagca agcagaagcg aagctgccgg aaggcagtca ggttctcttc gaacgggccg
     1441 tggaacggga caccggccgg gagtaccgca ttccatacct cgtcaaaaag cgggtcatgc
     1501 tgaccggaga cgtcttgagc gatgcgcgag tctcgatcgg gcaattcaac gatccatacg
     1561 tgtccattac ctttgacgca aagggtggac gcgaattcga gcggattacc ggcgagaacg
     1621 taaagaagcg gatggccatc gtcctcgaca acaccatcta ttcggcgccg gtcatccagg
     1681 agcggatcag cggggggaga gcgcagatca cgggtacttt ttcaacccag gaagccaacg
     1741 acctggcgat cgtgttgagg gccggggcgt tgccggcgcc gttgaagatc attcaggatc
     1801 tgacggtcgg cccgtcactg gggaaagatt cgattgaaaa gggcgtgcgg gccaccctca
     1861 ttgcaggggc cacggtgatc gtctttatga ttgtttatta ccggctttcg ggcgtgatcg
     1921 ccaactttgc gctcgtcctt aatctgatct gtttgatggg agccttatcg gcgctgaacg
     1981 ccacgctcac gctgcccggc attgccggga tcgtgctgac gatcgggatg ggtgtcgatt
     2041 ccaacgtcct gatcttcgag cgcatccgtg aagaactccg agcagggaag gcggtccgat
     2101 tggcggtcga cggaggctac gacaaagcgc tgctgacgat cgttgattcg cacgtaacca
     2161 cattaatcac tggtgtggcc ctgtttttgt tcggcacggg gccgatcaag ggattcgccg
     2221 ttaccctctg cctgggaatc gccatcaacc tgtttaccgc ccttgtcggg accaaggtca
     2281 tcttcgacat tatcaatcaa cgtcacaaag ttgagcagct gagcatttag tgtgaaggag
     2341 cctgcatgtt ggaaatcctg gggaagacga acttcgattt catgggcaag cgccggtatg
     2401 cgtttctgtt ctccggcatc atggtggtgc tcggcatcat cggattgatt cagatcgctc
     2461 gaggagcggc caatttgggc atcgactttg ccggtggcac ggccgtgcag ttgaagttcg
     2521 atcagccgat cgggatcgag gaagcccgaa aggcgcttga gaccaacggc ctgggcaatg
     2581 ccgaacttca ggaattcggc caggagaaca agctgctgat ccgtgtgaag gcctcgacga
     2641 ccatcgagga gaaaacggcg gagaaggtgg tgtcgatctt cacgaaggaa tttccaggga
     2701 accgcttcgt ggtagagtcc agcacggaaa tcggtccgac gatcggcaag aagctgcagt
     2761 cggatgcact cgtggccatc gtcatttcat tcgcgggcat catcctctac attgcggcgc
     2821 ggttcgagtt gcggttcggc gtcgcggcgg ccctggcgac ctttcacgac gtgctcgcag
     2881 tactaggggc cttctttcta ctcgacaaag aaatcaccct gctggtcatc acagcgttgt
     2941 taaccctcgc aggctattcg ctcaccgaca ccgtcgtggt cttcgaccgt atccgtgaga
     3001 acttgcgtct gcgccgccgc gagcacgaag tgacgatgat caacaatgcg atcaatcagg
     3061 tgttgagccg cacgattgtc accagtctga cggtcgtatt ggtgttgatt ccgttgacgc
     3121 tcggcggcgg cgaggtgctg catgactttt cgctggcgct gctttggggc gtcattttcg
     3181 gcacctattc ctcgatattc gtcgccagcc ctctgctgtt gttgtggccg gggggagcag
     3241 gacggctcct gaagcgggca taacacggca tgggcaaagc gcaagtcttg gcgtgcgctg
     3301 ggcgggcggg tgagatgggc aaaatcgcac gtcctgggta atcgatttga agcaatcggt
     3361 cggtggaccg ggtaaaaaac gattgcgcgg ggcgggcggg tgagctgggc aaagcgcagg
     3421 tcttggcgtg cgcggggcgg gcgggtgaga tggacgcgct ttttgaagac catgtaaaat
     3481 ctggtcgtcc cgacgaaggt acgcttgtga tctaggcgga ttatcatggc attctggagt
     3541 cgttcacaca gtctccagcg caagatcatc atggccatcg tgatggtcgg gttcctccca
     3601 ctgacgttcc tacttggcct cacctatttc gaagaacgcc gcgcgttgcg cgagactac
//
LOCUS       DASTQY010000429         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_219818, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000429 DASTQY010000000
VERSION     DASTQY010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219818"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..471
                     /locus_tag="VFR82_13825"
     CDS             217..471
                     /locus_tag="VFR82_13825"
                     /inference="COORDINATES: protein motif:HMM:NF021169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HEU4502728.1"
                     /translation="MQTVHVFDTWVTTPDHTLHFDVMTADQATALRLANAYVAAQGHT
                     AVSVTAEECRFCHAEPLVLFSEQQQREFRESGGFIVPLSA"
     gene            528..971
                     /locus_tag="VFR82_13830"
     CDS             528..971
                     /locus_tag="VFR82_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEU4502729.1"
                     /translation="MGKGIVGSLVLCLIVSTGIIAWAEGGSKGKSLYERLGGKGAITA
                     VVDTFVGKVGADKRINGYFASTDLGKLKTHLVNQICEASGGPCQYTGRTMKQTHAGMG
                     VTDAAFGALVEDLVAALDHHKVGKAEKDELLGVLGPMKKDIVEKP"
     gene            1141..2151
                     /locus_tag="VFR82_13835"
     CDS             1141..2151
                     /locus_tag="VFR82_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU4502730.1"
                     /translation="MARRLSPAKIMHLGIGFWGSKALLSAIELGLFTALAKGPCDADT
                     LRRQLGLHPRSSRDFFDALVALGMLKRSGQRYANTPETACFLDRNKASYVGGMLEMAN
                     ARLYRFWGSLTEGLQTGKAQNEVKTGEDFFGTLYSDPQRLEGFLKAMTGLSIGSARVI
                     AKKFPWRQYRTFVDVGCAQGGVAVEIALAHKHLTGFGMDLPVVQPVFEAYAKARGVAS
                     RLRFTAGDFFKDPLPHADVIVMGHILHDWDLRDKMMLLRKAYEALPARGALIVHEALI
                     DDDRKTNAFGLLMSLNMLIETHGGFDFTGADCRKWMKEAGFSRTRVASLAGPDGMVIG
                     FK"
     gene            2194..>2764
                     /locus_tag="VFR82_13840"
     CDS             2194..>2764
                     /locus_tag="VFR82_13840"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU4502731.1"
                     /translation="MHRDRPDESDGHDGSTSAPSVDRRRFLAGSAALIAQGSATLVFG
                     AAPADVTEGDPMRTPGSPARLYGDRSPFEASVRTGGGSHSLTPLEHLHGIVTPSSLHF
                     ERHHNGVPAIDPSRHRLLIHGLVDRPLIFTINELQRFPSVSRHAFIECAGNSRPAWLE
                     AQDLTVQELHGLTSTSEWTGVKLSTLLEAT"
BASE COUNT          577 a          788 c          825 g          574 t
ORIGIN      
        1 tacgtcctcc gccgcctgag ctcacaactc cagcgctgac ccggaatcta cccctacacc
       61 ttgcgacgat cttccaaccg cggattgagg gactgaccca aatccgttcc cctccctcaa
      121 gctgagaaca gagtgccgcc ctaccgctct aaagaggggc ttgaccgtac ccgaccctaa
      181 ctgctactaa ataggaatac taattaaagg ggcaggatgc agactgttca tgttttcgac
      241 acgtgggtca caactcctga tcacacgctg cacttcgacg tcatgaccgc cgatcaagcc
      301 acggctctga gactcgccaa tgcgtatgtt gcggcccaag gccacacggc ggtctccgtg
      361 acagcagagg agtgccggtt ctgccatgcc gaaccgttgg tcttgtttag tgaacaacag
      421 cagcgggaat ttcgtgaatc cgggggattt attgtgccgt tatccgcgtg aaggcggaca
      481 ggttggcggg ggggtatgta agtctcatca ccacaaggag ggcagccatg ggcaagggca
      541 tcgtcggttc attagtcctg tgtttaatcg tctcaacagg gatcatcgcc tgggctgagg
      601 gcggctcgaa ggggaagtcc ctctacgaaa ggctgggagg caagggagcg attacggcgg
      661 tcgtcgatac cttcgttggg aaggtcggtg ctgataagcg catcaacggg tattttgcga
      721 gtactgatct ggggaagtta aaaacacacc tggttaatca aatatgcgaa gccagcgggg
      781 ggccctgcca gtacacggga cgtacgatga agcagaccca tgccggtatg ggcgtgactg
      841 acgccgcgtt cggcgccctc gtggaagatc tcgtcgccgc cctcgatcac cacaaggtcg
      901 gcaaggccga gaaggatgaa ttgctcggcg tgctggggcc tatgaaaaag gacatcgtcg
      961 aaaagcccta gaccgtcaag cgccgggagg tttcgacctc ccggttattg tctcgtctag
     1021 gatatccacg cgatcctctc ggattcctct tcagatttgt ctccttggtc atcctgcctg
     1081 ttgacaccca gccgggcagt gcgtagcctg tctctatttt tgcagaaaaa gggacaacct
     1141 atggccagac gcctctctcc cgcaaagatc atgcacctcg gcatcggatt ttgggggtcc
     1201 aaggcgttgc tgagtgccat cgaactggga ctcttcaccg cattggccaa gggaccatgc
     1261 gatgcggata cgttgagacg acagctggga cttcatccga gaagctcgcg cgacttcttc
     1321 gatgcgctcg tcgcattggg catgctcaag cggtctgggc agcggtatgc gaacacaccg
     1381 gagactgcct gcttcctcga ccgcaacaag gcctcctacg tcggcggcat gctggaaatg
     1441 gctaacgcgc ggctttatcg tttttggggc tcgttgacag aggggttaca aaccggtaaa
     1501 gcgcaaaacg aagtaaagac cggcgaggac tttttcggta ccttatattc cgatccgcag
     1561 cggcttgagg gatttctcaa ggccatgacg gggctgagca tcgggtccgc gcgggttatc
     1621 gccaaaaagt ttccctggag acagtaccgg acgtttgtcg atgtcgggtg tgcgcaaggc
     1681 ggtgtggctg tcgagattgc actggcgcac aagcatctca ccggcttcgg catggatctg
     1741 cccgtggtgc aaccggtgtt cgaggcttat gccaaggcga gaggcgtcgc ctcacgactg
     1801 cgtttcaccg ccggagattt cttcaaagac ccgctcccgc atgccgacgt catcgtcatg
     1861 ggccacatcc tgcacgattg ggacctccgc gacaaaatga tgctgctacg caaagcgtat
     1921 gaggccctcc ccgcaagggg cgccctgatc gtgcacgagg cattgatcga cgacgaccgg
     1981 aagaccaatg cctttggcct gctcatgagt ctgaacatgc tcatcgaaac gcatggcggt
     2041 ttcgacttta cgggggccga ctgtcggaag tggatgaagg aagccggttt cagtcggact
     2101 cgcgtcgcgt cattggcggg gccggacggc atggtgatcg gattcaagta agcgccacga
     2161 ccctcatttt catcatcaga aaggagaatt ccggtgcaca gagaccgccc cgacgagtct
     2221 gatggacacg atggttcgac gagtgcgccc tcggtcgacc gccgtcggtt tctagccgga
     2281 agcgcagcgc tcatcgctca ggggtccgcc acgcttgtat ttggtgcggc gccagccgat
     2341 gtcactgaag gagacccgat gcggacacca ggctcgcctg cccgactgta tggcgaccgg
     2401 tctccctttg aagcatcggt cagaacgggc ggaggctctc actcactcac cccgttggaa
     2461 catttgcacg gtatcgtgac gccatcttcc ttgcattttg agcggcacca taacggcgtg
     2521 ccggctatcg acccctcgcg gcaccgatta ttgattcacg gattggtgga ccgaccgctg
     2581 atcttcacga ttaatgagct gcagcggttc ccctcggtct cgcggcacgc gttcatcgaa
     2641 tgtgccggaa atagccgccc ggcttggctg gaagcacaag acctcacggt tcaggagctt
     2701 catgggctga cgagcacgag tgagtggacc ggggtcaaac tctcaacact gctggaggcg
     2761 acgg
//
LOCUS       DASTQY010000430        13069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_501984, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000430 DASTQY010000000
VERSION     DASTQY010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 13069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13069
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501984"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="VFR82_13845"
     CDS             <1..1483
                     /locus_tag="VFR82_13845"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU4502732.1"
                     /translation="FGVPTSLDNPANAARFLSPVLHIRREEIEKKLRQDKHFVWLARK
                     VEPEQGHRLEQLSIDGIGMVMEGRRFYPKGPLLAHVLGFVGMDGIGLEGLERRYETQL
                     HGEKRMTILQRDALGRTVFPKSLREQVPAAGHALTLTIDEVIQYIAEKELEEAVDHSR
                     AKSGTIIVLEPKSGAILALAVSPRFDPNAVAALTADRWRNRALTDTYEPGSTMKVVVA
                     AAALEERVMMPGSMLFGENGRMTIANTTIHDHEKLGWMSFAQMIQKSSNIGAAKTGML
                     LGDQRLYRYLQAFGFGQRTDVDLPGEVAGLLKSPREWGRRSLASISMGQEVGVTPLQM
                     VSAVAAIANDGVLMKPYVVSGVRDQKGQLVKEIFPHVKRRVVSPVTAQTLTTILEGVV
                     TTGTGTKAAIPGFRVAGKTGTAQKVDPRTGAYSSALSIGSFVGFVPADSPRLAMIVVI
                     DEPQGEAWGGVVAAPVFRRVGEQVLTYLGVSRDDQVKIAMVGP"
     gene            1495..3015
                     /locus_tag="VFR82_13850"
     CDS             1495..3015
                     /locus_tag="VFR82_13850"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU4502733.1"
                     /translation="MQPRSLTVTQLLVALNGQVAIVGRQGALDGSITSVTDDSRSVAL
                     GSLFVAVKGERVDGHEYVGQAIKAGAAAIVGQESVERGSVPFVQVADSRKALGLIGSR
                     FYGDPSAQLAMIGVTGTNGKTTTTYLCKALLENIGRRVGLIGTVAYQIGAETIPASHT
                     TPGALELQQLLARMSEAQLDSVVMEVSSHALAMDRTAGCEYDVAVFTNLTQDHLDYHR
                     TMEEYFQAKLRLFTGLGQGKKTRQRAIVNMDDPRGVYVRAACRVPVWGYALNASADLM
                     AENVRLSMNGSTFSAATPAGTFTVESRLVGEHNVYNLLGAIGAALHGGATCDQVREAV
                     ARVTNVPGRFERVSAGQDFTVVVDYAHTEDALVRLLTAARTLNAQRIITLFGCGGDRD
                     RGKRPKMGRAAVEFSDVVVLTSDNPRTEDPLAILREVESGVREALQRRPSVQYHMVAD
                     RREAIRTAVRLARPGDILLIAGKGHEDYQIVGTKKMHFDDREVAREAIQQLRKNCA"
     gene            3012..4508
                     /gene="murF"
                     /locus_tag="VFR82_13855"
     CDS             3012..4508
                     /gene="murF"
                     /locus_tag="VFR82_13855"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU4502734.1"
                     /translation="MKFHVGGSTERREDSMALLTVEELREVISVKVLAGDPSSSTRRR
                     IRNISTDSRSIRRGDLFVALKGERFDGHDFVRAVLAKGATGAIVHDTYHWDAKTGKSS
                     GISRHEPFLFGVRDPLFAYQQLATHHRCRFAIPVVAVTGSNGKTTTKDMVASVLGQRW
                     RTLKTEGNFNNRIGVPHTLLRLTARHQAAAIEMGVDHRGQTTRLCEIVRPTIGLITNI
                     GPDHLEFFGGMEGSAQAKAELLDMLPADGTAVLNADDAYFDYLAARAQCRVISFGLSE
                     RANVRASELSTDARKGTTFELVLPGRSRPTSVTIKVLGTHNVTNALAAAAVGFSLNLS
                     GAVIAQGLARFRPAAMRSQVVTHQGVHIINDCYNANPASMKAALKVLADWTPARARVA
                     VLGDMLELGRQTLPLHREVGQFAAALKLTRLIVCGDLGREIAVGAREAGMSNQVITQL
                     PDASSAADLLRKTVRQGDVVLVKASRGMRMEQIVQGLTGMKAVTRQAS"
     gene            4539..5615
                     /gene="mraY"
                     /locus_tag="VFR82_13860"
     CDS             4539..5615
                     /gene="mraY"
                     /locus_tag="VFR82_13860"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU4502735.1"
                     /translation="MLYNLLYPLHTEYGFLNVFRYLSFRIIYAAVTAFLIAFVLTPWL
                     IRKLQEIKLGQQIRDDGPKHHVAKSGTPTMGGLLIIFAVLLSTMLWADITKPYVWLVM
                     IATLGFGAIGFVDDYLKFIKARSKGLSPTQKFSAQIAVALLIAVALYLLPSYSTRLNV
                     PFFKNFMPDLGWFYIVFVVLVIVGSSNAVNLTDGLDGLAIGPIMIAALAYTIVAYVAG
                     HRIMSDYLLIPHIDGAGEIAVFTAAILGSSLGFLWFNTYPASVFMGDVGSLPLGAALG
                     TVAVISKHELLLLMVGGVFVIEAVSVIFQVASFKSRGKRIFLMAPIHHHFEMKGWEEP
                     KVVVRLWIIAILLALLSLSTLKLR"
     gene            5621..7030
                     /gene="murD"
                     /locus_tag="VFR82_13865"
     CDS             5621..7030
                     /gene="murD"
                     /locus_tag="VFR82_13865"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU4502736.1"
                     /translation="MLEVEEVDLSGAQVTVLGLARSGVAACHLLRETGARVTVADRKD
                     EVELAAVLRTVDRNLVQVSVGSQYERSLDRADLVVISPGVPYRMEALERVRRRGVKVI
                     SELELASRFLSAPILAVTGTNGKSTTVTLIGKMLAEQGKRAFVGGNLGTALSEAAWLT
                     LQAKKQGKPDPYEYLAVEVSSFQLETIDRFHPWAAAILNVTVDHQDRYDSIEEYVAAK
                     RRIFENQTNDDFALFNLDDPRVANLTSTVQARRLGFSRTQPIGAGFDGGTYLDGDEIV
                     TTVAGGRQRICRKSELKIIGHHNVENVMAAATYAALWGCPLDIISRVVTAFPGLEHAL
                     ELVRERRGVRFVNDSKGTNVDATLKALEGIERPIWLIAGGRDKGGDFSRLTEAIRRRV
                     KHVILIGEAAPLLKAAWTGATTMSEAESLRGAVEWAAREAAAGDVVLMSPACASFDMF
                     QDYQDRGRQFKSAVESLPE"
     gene            7140..8249
                     /gene="ftsW"
                     /locus_tag="VFR82_13870"
     CDS             7140..8249
                     /gene="ftsW"
                     /locus_tag="VFR82_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEU4502737.1"
                     /translation="MDHVLLFVTLTLALMGLVMVFSASAIVAGNRFQDPEFFLKRQIA
                     WLGFGFLLMHLTSRIDYQLWKKLSMPILICMAILLVMVLIPSLGVAAKGARRWLRWGP
                     ISIQPAEMVKLVSVIYLATYLAKKADKLTLFRSGLLPALIVIAVLSGLVLLEPDLGTV
                     VVMGLVTVSLLFLGGARIGHLVSLGLCAIPVVFVLVLSSSYRRQRLMTFLAPWKDASD
                     AGFQITQSFLAFGSGGPFGVGLGEGKQKLFFLPEAHTDFVLALVGEELGLAGTAAVIV
                     LFALFVWRGFHIAARARMPFGKYLAMGITLLIGIQALVNAAVVTGLLPTKGLTLPFVS
                     YGGSSLVVSFVGVGMLLSISRDRHGGASKGGRGGD"
     gene            8252..9376
                     /gene="murG"
                     /locus_tag="VFR82_13875"
     CDS             8252..9376
                     /gene="murG"
                     /locus_tag="VFR82_13875"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEU4502738.1"
                     /translation="MTIVIAAGGTGGHLYPAVALAREFLHRDPMTKVLFVGTSRGLES
                     KVLAHEGFELVLINAKPVMGKGLTDVLKGLCAMPLSLWQCARILNQRQARLVIGVGGY
                     TSPMMVLAAAMKGVPRVILEPNAYPGLANKAVGPFVQRVFLAFESAARFFDQRKGRVV
                     GTPVRKEFLAQSRMPRGERQRGRCHLLVFGGSQGAKALNSAAIEGLPDLLKQRPQLTV
                     THQTGEADHDRVSQAYRRAGVTATVLPFVYDMPAAINAADVVVARAGAMTVAELTTCG
                     KPAILVPLPTAIYDHQMKNAKVMEAAGAAVVLPQADLTGATLARAVAHILDDPERMKA
                     MEDASVGLRRLDAAEAIVRECYALIGDQRHVNHSLGAAGI"
     gene            9342..10832
                     /gene="murC"
                     /locus_tag="VFR82_13880"
     CDS             9342..10832
                     /gene="murC"
                     /locus_tag="VFR82_13880"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU4502739.1"
                     /translation="MSTILLEQQGSDGTGQRNGFTSSQAAGGLRRTSMFRKTQQIHLV
                     GIGGSGMSGIAEVLLTLGYKVTGSDQQASDTTRRLEELGGRIFIGHHESQIGEAQVVV
                     ISSAVSPQNPEVMAAKAKQIPVIPRAEMLAELMRLKFGVAIAGAHGKTTTTSMVANVL
                     AQGGLDPTMVIGGKVNALGSHARLGRGELLVAEADESDGSFLRLSPTIIAVTNLDREH
                     LDHYGSMERIYESFLEFINKVPFYGLAVLCADDHRLRGLFPNIVKRYYTYGLSEPDGV
                     APDFRATDIVAKQGGSEFRAFFRTRNLGPFRLAVPGVHNVSNALAAIAIGVELDVPVD
                     LIRKALAAFTGVERRFHFRGESNGVMVVDDYGHHPTEIKATLAAAKQGWPERRLVVLF
                     QPHRYTRTRDLLEDFSKAFQHADRLFLTEIYAASESPIPGVSGARLTEAIRAVGHPPV
                     TFVERKEQLTEQVLPQLKAGDLVITLGAGDIWKTGPALLEKLAAAS"
     gene            10912..11784
                     /gene="murB"
                     /locus_tag="VFR82_13885"
     CDS             10912..11784
                     /gene="murB"
                     /locus_tag="VFR82_13885"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU4502740.1"
                     /translation="MANLRGRIHFDASLETYTSFKIGGPADVLVEPADVDDVRRLVSQ
                     ASAARMPIFVLGGTNLLIRDGGIRGIVVSLSKLRGIHEEPGAVLYAEGGVGMPTLIGH
                     AIRRSLSGLEWAAGIPGTVGGCLVMNAGTRLGEMKDAVKAVRLITMKGELRDLPASSI
                     PFQYRRARLPKGIVVGVWLQLRPALRADIERVVKEYLHYRKDTQPLTMPSAGCVFKNP
                     PDDSAGRLLEAAGLKGARVGDAEVSMKHGNFIVNRGHASAADVIQLIGKVRQTIKRKA
                     GVRLDLELKIVGEA"
     gene            11812..12789
                     /locus_tag="VFR82_13890"
     CDS             11812..12789
                     /locus_tag="VFR82_13890"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU4502741.1"
                     /translation="MTERRRLTQARIGVLMGGQSSEREVSLRTGAAVYGALCRLGYDA
                     VTVDVGPTLSRDLQDQKIAVAFLALHGPGGEDGSIQGFLETVGIPYTGSGVHASAVGM
                     HKVTTKTVLAAHDIPVPAGLVIKRGERVTHVQALRSAKLRWPVVVKPASEGSTIGVSI
                     VKKPAQWNPALSLAHRYDRDAMVEAYIPGHEVTVSLLGRENETPLVLPAVEIVAPGGF
                     YDFSAKYEKGKTQYLCPAPLSAAVTKEIRELALRAFEVLGCAGAARVDFRITPRGRPY
                     VLELNTVPGMTETSLLPMAAVQAGIGYEELTERILESALARMKNKEHAA"
     gene            12786..>13069
                     /locus_tag="VFR82_13895"
     CDS             12786..>13069
                     /locus_tag="VFR82_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502742.1"
                     /translation="MRLLTRKRPAKAAGYRSNEWKGPRAAQSMDHRQRARRIERLACC
                     RRALCLSGWLVGAGAVCWTILMLSQQLGPVLHRGLEIKHVSVEGTHQVTK"
BASE COUNT         2694 a         3508 c         4099 g         2768 t
ORIGIN      
        1 cttcggggtc ccgacatcgc tcgacaatcc agccaacgcc gctcgattcc tgtcgccggt
       61 cctgcacatt cgccgcgagg aaatcgaaaa gaagcttcga caggacaaac attttgtgtg
      121 gctggccaga aaggtcgagc cggagcaggg tcaccgattg gagcagctgt caatcgacgg
      181 catcgggatg gtgatggaag ggcggcggtt ttatccaaag ggtcctctgc ttgcccatgt
      241 gctgggattc gtcggcatgg atgggatcgg tcttgaaggt ttggaacgtc gctatgaaac
      301 gcaattgcac ggggaaaaac gaatgacgat tctccagcgc gatgcgctgg gacgaacggt
      361 gtttccaaaa agtctccggg aacaagtgcc tgccgcaggt catgcgctca cactgacgat
      421 tgacgaggtc attcaataca tcgccgaaaa ggagttggag gaggctgttg accattctcg
      481 cgccaaatcg ggaaccatca tcgtcttgga gccgaaaagc ggagcgatcc ttgccttggc
      541 cgtcagtcca aggtttgatc caaatgcggt agccgcgctg accgccgatc gttggcggaa
      601 tcgggctttg acggacacct acgagcctgg ctcgacgatg aaggtggtag tagcggctgc
      661 cgctctcgag gagcgagtca tgatgcccgg ctcgatgttg ttcggcgaaa acggcaggat
      721 gacgatcgcg aatacgacca ttcatgacca cgagaagctc ggctggatga gcttcgctca
      781 gatgattcaa aagtccagca atatcggcgc ggcaaaaacg ggcatgctgc tcggtgatca
      841 acggctgtat cgctaccttc aagcgttcgg gtttgggcaa cgtacggatg tcgatcttcc
      901 cggtgaagtt gctggattgc tgaagtcgcc gcgcgaatgg ggccgtcgct cgctcgcttc
      961 gatctccatg ggacaggagg tcggggtgac gcctttacag atggtttccg ccgtcgccgc
     1021 catcgccaat gacggcgttc tcatgaagcc atatgttgtt tcaggagtga gagatcagaa
     1081 ggggcagctc gtgaaggaaa tttttcccca cgtgaaacgc cgcgttgtct cccccgtcac
     1141 cgctcagact ttgacgacga tcttggaagg ggtggtcacg accggaacgg gcacgaaagc
     1201 ggcaattcca ggatttcgtg tggcaggtaa aaccgggacg gcacaaaaag tggatccacg
     1261 tactggagcc tattccagcg cgctctccat cgggtctttt gtgggcttcg ttccggctga
     1321 ttctccgagg ctggcgatga tcgtcgtgat cgatgaaccg caaggcgaag cctggggtgg
     1381 agtcgtggcg gcacccgtat ttcgccgtgt cggagaacag gtgcttacct accttggtgt
     1441 gtcgcgagac gaccaggtga agattgccat ggtcggtcca tagtgaaggt gacgatgcag
     1501 ccgcgatcgc tgacggtcac gcaattgttg gtggcgttga acggtcaagt ggccattgtt
     1561 gggcgccagg gagccttgga tggctcgatt acctccgtga cggacgattc gaggtccgtt
     1621 gccttgggga gcctcttcgt cgccgtcaaa ggcgagaggg tcgatggcca cgagtatgtc
     1681 ggccaagcga ttaaagccgg agcagcggcg atcgtcggac aggaatccgt agaaagggga
     1741 tcggttccgt tcgtgcaggt ggcggattca cgcaaagctt tgggtttgat tgggagccgc
     1801 ttttatggtg atccctcggc gcaactggcg atgatcggtg tgaccgggac gaatggaaaa
     1861 acgacaacca cctatttgtg caaggcgcta ttggagaaca tcggtcgtcg ggtgggactg
     1921 atcgggactg tcgcctatca gatcggagcg gaaacgatcc cggcgtcgca cacaacccct
     1981 ggagcgttgg aattgcagca attgctggcc aggatgagtg aagcacagtt ggatagcgtc
     2041 gttatggagg tgtcctcgca tgcgttggcc atggatcgaa ccgcaggatg tgagtacgac
     2101 gtcgccgtct ttacgaactt gacacaggat cacctcgatt atcaccgcac gatggaagaa
     2161 tactttcaag cgaagctgcg gctgttcacg gggcttggcc aaggaaaaaa aacgaggcag
     2221 cgagccatcg tcaatatgga cgatcctaga ggggtctatg tccgagctgc ctgccgagtt
     2281 cccgtatggg gctacgccct caacgcctcg gccgatctca tggccgagaa cgttcgcctg
     2341 tcgatgaacg gttccacgtt ttccgctgcc actcccgccg gcaccttcac ggttgaaagc
     2401 cgcttggtgg gcgaacacaa cgtgtacaac ttgctcggcg cgatcggtgc ggcgctccac
     2461 ggaggggcta cctgcgatca agtccgtgag gccgtggccc gagtgaccaa cgttcctggt
     2521 cggttcgagc gcgtgtcggc cggacaagac tttaccgtcg tagtggatta tgcccacacc
     2581 gaagatgcgc tggtgcggct cttgaccgcc gcgcggacgc taaacgcgca gcggattatc
     2641 accctatttg ggtgcggtgg tgatcgagac cgcgggaagc gtccgaaaat gggccgcgcc
     2701 gccgtggaat tcagcgatgt cgtggtcctg acatcggata accccagaac agaggatccg
     2761 ctggcaatcc ttcgagaggt tgaatccgga gttcgcgaag cgctgcaacg tagaccgtcg
     2821 gtgcaatacc acatggttgc cgaccgtcgt gaagccattc gaacggccgt acgtctggct
     2881 cggccgggag acatcctgtt gatcgcgggc aaaggacatg aagactacca gatcgtaggc
     2941 accaaaaaga tgcacttcga cgatcgggaa gtcgcgcgtg aagccatcca gcagttgagg
     3001 aaaaattgcg cgtgaagttc cacgtgggag gaagcaccga acggcgagag gactcgatgg
     3061 cgctcttgac agttgaagag ctgcgcgaag tcatcagcgt gaaggtcttg gcgggcgatc
     3121 cgtcgtcatc tacaaggcga cgtattcgga acatctctac cgattccagg tccatacggc
     3181 gaggcgatct gtttgtcgcg ctaaagggtg agcggttcga cggtcatgat tttgtgcggg
     3241 cggtactcgc gaagggagcg acgggcgcca tcgttcacga tacgtatcac tgggatgcca
     3301 agaccggaaa atcaagcggc atctctcgtc atgaaccgtt ccttttcggc gttcgcgacc
     3361 cgctgtttgc gtatcaacaa ttggcgaccc atcaccgatg ccggtttgcg atacctgtcg
     3421 tagccgtcac gggcagtaac ggcaaaacca ccacaaaaga catggttgcc agcgtccttg
     3481 gtcaacgttg gcgtacgctg aaaaccgaag gtaacttcaa caaccggatc ggtgttcctc
     3541 acacgctgct acggctgaca gcccgtcatc aggcagctgc gatcgaaatg ggagtcgatc
     3601 atcgggggca aacgacccgc ctttgtgaaa tcgtacgacc gaccatcgga ctcatcacga
     3661 atatcggtcc cgaccacttg gaattcttcg gcggcatgga ggggtcggcg caagcgaagg
     3721 ccgagcttct cgacatgctc ccggccgacg ggacagcggt gttgaacgct gatgatgcgt
     3781 attttgacta tctggccgcc agggctcaat gccgcgttat ttcattcggt ttgtctgaga
     3841 gggcgaatgt ccgggcgagt gagctgtcga cagatgcccg taagggaacg acctttgagc
     3901 tggtgcttcc cggaagaagc cggccaacgt ccgtgaccat taaggtgctt ggtacgcaca
     3961 acgtcaccaa tgcgttggct gccgcagccg tcgggttttc attaaacctt tcgggcgcag
     4021 ttattgcgca gggactggcc cgattccgtc ctgcggccat gcggtcacaa gtcgtgaccc
     4081 accaaggcgt ccacatcatc aatgattgtt acaacgcaaa tcctgcttcg atgaaagcgg
     4141 cgttgaaggt cctcgcagac tggacacccg cgcgtgcacg ggtggctgtg ctgggagata
     4201 tgctcgaact cggccggcag accttgcctc tgcatcgcga ggttgggcaa ttcgccgccg
     4261 cgctcaagct caccagatta atcgtttgcg gtgacctggg gcgtgagatt gcggtcggag
     4321 cgcgcgaggc aggaatgtcc aatcaagtga tcacgcagct gcccgatgct tcctcggcgg
     4381 ccgacctctt gaggaaaacc gttcgccagg gagatgtcgt ccttgtgaag gcatctcggg
     4441 gcatgaggat ggaacaaatt gtgcagggat tgactggcat gaaggccgtc accagacagg
     4501 cgtcatagga agcgggcggc accgagatca aagaacgcat gttgtacaac ctcctatatc
     4561 cgcttcatac cgaatatggc ttcctcaatg tgtttcggta cctgagtttt cggatcattt
     4621 atgctgcagt gacggcgttt ctcattgcgt tcgtgctcac gccgtggctc attcgcaagc
     4681 tgcaagagat caagctcggg caacagattc gcgacgacgg gcccaagcat cacgtggcca
     4741 agagcggcac acccaccatg ggcggtctcc tgatcatctt tgccgtactg ctatcgacga
     4801 tgctgtgggc agatatcaca aagccctatg tctggctcgt gatgatcgcg acgttaggat
     4861 ttggagcgat cgggtttgtg gacgattact tgaagtttat caaggcacga tccaaaggcc
     4921 tctcgccgac acagaaattc agcgcccaga ttgccgtggc actgttgatc gccgtcgcac
     4981 tgtatttgct ccccagctat tccacgaggc tgaacgtccc attctttaag aatttcatgc
     5041 cagacctcgg gtggttctac atcgtgttcg tcgtcctggt catcgtcggc agttcgaacg
     5101 ccgtgaactt gactgacggg ctggacggtc tcgcgatcgg tccgatcatg attgcggcct
     5161 tggcctacac tatcgtcgcc tatgttgctg ggcaccgcat catgtcggat tatctgctca
     5221 tcccccatat tgatggcgcg ggcgagatcg ccgtctttac ggcggctatt ttggggtcga
     5281 gcctcggatt cctctggttc aatacctatc cggcatcggt ctttatgggc gacgttggtt
     5341 cgctgccgct gggcgcggca cttgggaccg tggcggttat cagcaaacac gaattattgc
     5401 tcctcatggt tggaggcgtg ttcgtgatcg aagcggtgtc cgtcattttt caagtggcgt
     5461 ccttcaagtc gcgcgggaaa cggatttttc tgatggcgcc gatccatcat cattttgaaa
     5521 tgaagggatg ggaggagcca aaggtcgtcg tgcgactctg gatcatcgcg atccttctgg
     5581 cgctcttaag tctgagcacc ttgaaactgc ggtgatggac atgctggagg tcgaagaagt
     5641 ggacttgtcg ggagcgcagg tgacggtgct cggactcgca cgaagcgggg tcgccgcctg
     5701 tcacctactc agggaaaccg gagctcgcgt cacggtagct gatcgaaaag acgaggtcga
     5761 attggccgcg gtccttcgga cagtggaccg caatctcgtc caagtgtcgg tcggctccca
     5821 gtacgaacgc tcgctcgacc gcgccgatct cgtcgtcatt agtccaggcg tgccttatcg
     5881 tatggaggca ctcgagcgcg tccgacgacg gggcgtcaaa gtcatcagcg agcttgaact
     5941 ggcctcccga ttcctctcgg cgccgatcct cgccgtcacg gggacgaatg gaaaaagcac
     6001 gaccgtgacg ctcataggaa agatgctggc cgagcaaggt aagcgcgcat tcgtcggggg
     6061 aaacctggga accgcgctca gcgaggcagc ttggctcact ctccaggcaa agaagcaagg
     6121 caagccggac ccctatgagt acctggcggt tgaagtgtcc agtttccaat tggagacgat
     6181 cgatcggttc catccctggg cggcggcgat cttgaacgtc acggtcgatc atcaggaccg
     6241 gtatgactca attgaagaat acgtcgcagc caaacggcgg attttcgaaa atcaaacgaa
     6301 cgacgatttc gcgctcttca atctcgatga cccgagagta gccaatctga cgagtacggt
     6361 gcaggccagg cgcctgggat tttccaggac acagccaatc ggcgccgggt tcgatggtgg
     6421 gacctatctc gacggcgacg aaatcgtgac gaccgttgct ggaggccgac agcgcatctg
     6481 tcgtaagagc gaactgaaga tcattgggca tcacaacgtg gagaatgtca tggctgcggc
     6541 cacctatgcg gccttgtggg gatgccctct cgatatcatc agccgcgtgg tgacggcgtt
     6601 ccccggcctg gagcatgcct tagagcttgt gagggaacgc cgtggcgtgc gttttgtgaa
     6661 tgattcgaag gggacgaacg tggatgccac tcttaaggcg ctggagggaa tcgagcgccc
     6721 aatctggctg atcgcgggcg gtcgcgacaa aggcggcgat ttttctcgtc ttaccgaagc
     6781 cattcgtcgc agggtcaagc atgtcatttt gatcggggaa gcggcacctt tattgaaggc
     6841 ggcttggact ggcgcgacga ccatgagcga agcggagtcc ttgcggggcg cagtagagtg
     6901 ggcagctcgc gaggcggcgg ccggtgacgt cgtgttgatg tcgccggcct gcgcgagttt
     6961 cgacatgttc caggactacc aagaccgcgg tcgccaattt aaatcggcgg tggagtcgct
     7021 accggaatag cggacgaaat caggaggact gactctgctc aatgggatct cgaactgcag
     7081 gaacgttatc gttgccttgg ccgacgacag gacaacgatc gggcaagtca cgagtagcga
     7141 tggatcatgt cctgcttttt gtgacgctga cactcgcgct catggggctg gtgatggtgt
     7201 tcagtgccag cgccattgtg gccggcaatc gattccagga tcccgagttc ttcttgaaac
     7261 ggcagatcgc ttggctggga ttcggtttct tactcatgca tctgacctcg cgcatcgatt
     7321 accagctgtg gaaaaagctt tcgatgccca tccttatctg tatggcgatc ctgctcgtca
     7381 tggtgttgat cccttcgctt ggcgtcgcgg ccaagggtgc caggcgttgg cttcgatggg
     7441 gtcccatttc gatccaaccg gcggaaatgg tgaagcttgt ctcggtgatc tacctggcaa
     7501 cctacctggc caagaaggcg gacaaattga cgctcttccg gagcggtctc ctgccagcat
     7561 tgatcgtgat cgccgtcctc agcgggctcg tgttgctgga accggatctc ggtaccgtcg
     7621 tcgtgatggg actggttacg gtgtctctgc tgttcctagg aggtgcgcgg ataggtcatc
     7681 tggtcagcct cggtctctgt gcaattcctg tggtcttcgt gctcgtccta agctccagct
     7741 acagacggca acggctcatg acctttctcg ccccatggaa agacgcttcc gacgccggat
     7801 tccagatcac ccaatctttt ctcgcattcg gcagcggcgg cccttttggc gtcggcctag
     7861 gcgaagggaa gcagaagttg ttttttttac cggaagcgca taccgatttc gtgctggcac
     7921 ttgtagggga agaactcggc ttagccggta cggcggcggt cattgtcctt ttcgcactct
     7981 ttgtgtggcg agggttccac atcgccgcgc gggcgcggat gccgttcggc aagtatctcg
     8041 caatgggtat cacgctgtta atcgggatcc aagcattggt caacgccgct gttgtaaccg
     8101 ggttgttgcc gaccaagggg ctgacgctcc cttttgtcag ctacggcggg tcatcgctcg
     8161 tcgtgagttt tgtcggcgtc ggaatgcttc tgagcatttc gagggatcgg catggcggtg
     8221 catctaaagg cggacgtggt ggggactaag aatgaccatc gtcattgcag ctggaggaac
     8281 gggaggacat ctgtatccgg ccgtggcgct ggcgcgggaa tttctgcacc gcgatccgat
     8341 gacgaaagtc ttgttcgtgg ggacatcacg cggtctcgag tccaaagtgc tggctcacga
     8401 gggattcgag ttggtcttaa ttaatgcgaa gccggtgatg ggcaaaggac tcactgacgt
     8461 gttgaagggg ttgtgcgcca tgccgctcag tctatggcaa tgcgctcgga tcctgaatca
     8521 acgacaggcg cgtctcgtca tcggtgtcgg agggtatacc agtcctatga tggtactggc
     8581 agccgcgatg aagggcgtcc cccgggtgat tctggaacct aatgcgtatc ctggtctggc
     8641 caataaagcg gtcggcccat tcgttcagcg cgtgtttctc gcatttgaat cggcggcgcg
     8701 tttcttcgat caacgcaaag gacgtgtcgt aggaacaccg gtccggaaag agtttctcgc
     8761 tcaatctcgt atgcccagag gagagcgcca gaggggccgt tgccacctgc tggtctttgg
     8821 cggcagtcaa ggcgccaaag ccctcaacag cgcggcgatc gagggcctcc cggatcttct
     8881 taaacaacgc ccgcaattga ccgttacgca tcagacgggc gaggcggacc atgatcgggt
     8941 gagtcaagcc tatcggcggg ccggagtaac agcgactgtc ctgccgttcg tgtacgacat
     9001 gcccgcggcc atcaacgccg ctgatgtcgt cgtcgcgcgt gccggcgcca tgacagtggc
     9061 ggagctgaca acttgcggca agccggccat tctcgttccg ttgccgactg caatttatga
     9121 tcatcaaatg aagaatgcca aggtcatgga agctgcaggc gcagcggtgg tgctgcccca
     9181 ggcggacctt accggcgcga ctctggcacg ggcggtggcc cacatcctcg acgaccccga
     9241 gcggatgaaa gcgatggaag atgcgagtgt tggcttgaga cgattggacg cggctgaagc
     9301 catcgtccgt gaatgctatg cactcatagg agaccagcgt catgtcaacc attctcttgg
     9361 agcagcaggg atctgatggg acagggcaac gcaacggctt cacctcctcg caggcagctg
     9421 gtggtctgag gcggacatcg atgtttcgga aaacacaaca aattcatttg gtggggatcg
     9481 gcggctcggg catgagtggg atcgccgagg tgttgctgac cctcggctac aaggtgaccg
     9541 gctccgatca acaggcctcc gataccaccc ggcggttgga ggaattggga ggccggattt
     9601 ttatcggcca tcacgaatcc cagatcggag aggcgcaagt tgtggtgatt tcgtccgccg
     9661 tgtctccgca aaacccggaa gtgatggcag ctaaggctaa acagattccg gtcatcccgc
     9721 gagccgaaat gctcgccgaa ctcatgcggc tgaaattcgg cgtggccatc gccggcgctc
     9781 acggaaagac caccaccacg tccatggtgg caaatgtcct ggcgcaagga ggattggatc
     9841 ccacgatggt gatcggaggc aaggtgaatg ctttggggag ccatgcacgg ctgggtcggg
     9901 gagagctgct cgtagcggaa gccgatgaga gcgacggatc gtttttgagg ctgtctccaa
     9961 ccattatcgc tgttacgaac ctggaccgtg aacatctgga tcactacggg agcatggaac
    10021 gtatttatga aagcttcctt gaattcatca ataaggtccc gttctacggg ctggcggtgt
    10081 tgtgtgccga tgatcatcgg ttacgggggc tgttccccaa tatagtcaaa cggtattaca
    10141 catacggctt gtcggagccc gatggcgtgg cacccgattt tcgtgcaacc gacatcgtgg
    10201 ccaaacaagg cggttccgaa tttcgcgcat ttttcaggac aaggaacctg ggaccgtttc
    10261 gcctggcggt accgggcgtt cacaacgtat cgaacgcttt ggcggcaatc gccattggcg
    10321 tcgaactgga cgtgccggtg gatttgatcc gcaaggcatt ggcagccttt accggtgtgg
    10381 agcggcggtt tcattttcgt ggagaatcca acggtgtcat ggtcgtggac gactatggcc
    10441 accacccgac tgaaatcaaa gcgacgctgg cagccgcgaa gcaagggtgg cccgagcggc
    10501 gtctggtcgt gctctttcag ccgcaccgct acacccggac cagagacttg ttagaagatt
    10561 tttccaaagc gtttcaacat gccgaccgcc tctttttgac ggagatctat gcggctagcg
    10621 agtcccctat ccccggagtg tccggcgcca ggttaacgga ggcgattcgt gctgtcggcc
    10681 atccgcctgt cacctttgtc gagcggaagg aacaattgac cgaacaagtg cttcctcagt
    10741 tgaaggccgg cgatctggtg atcacgcttg gggcgggaga catctggaag acagggcctg
    10801 cattactgga gaagctggcc gctgcttcat gatgcgatga acgccgatgg acgaagaacg
    10861 aagcgactta gtgcgagatc ccgattcgac cgcaagcgtc tggaagccgt cgtggcaaat
    10921 cttcgcggtc gcatacactt cgacgcgtcg ttggagacct atacctcgtt caagatcggc
    10981 gggccggctg atgtgctggt ggagccggcc gacgtcgatg atgtgcgccg gctcgtcagc
    11041 caagcgagcg cagcacgtat gcccatattc gtgctcggcg ggacgaatct tctcatccga
    11101 gacggaggga ttcgcggcat cgtggtgagt ttgtcaaagc ttcgcgggat acatgaggag
    11161 ccgggagcgg tcctctatgc cgaaggcggc gtcgggatgc caaccttgat cggtcatgcc
    11221 atccgccggt cattgtccgg cttggaatgg gcggccggca ttcccgggac cgtcggaggc
    11281 tgtctggtga tgaacgccgg aacaaggttg ggcgaaatga aagacgccgt gaaggcggtc
    11341 aggctaatca ctatgaaggg tgaactccgc gatctaccgg cttcgtccat tccatttcag
    11401 taccgcaggg cccggctgcc gaaggggatc gtggttggag tgtggctcca gttgcgcccg
    11461 gctttgcgcg ccgacatcga acgagtggtc aaagagtacc tccattatag gaaggatacc
    11521 cagccgctca cgatgcccag cgccggctgc gtgttcaaga atcctcctga tgattcggcg
    11581 ggtcggctgc tcgaagcggc agggttgaag ggcgctcgag tcggtgatgc ggaggtatcg
    11641 atgaagcatg gcaacttcat cgtcaaccgc ggacatgcga gtgccgctga tgtcattcaa
    11701 ttaatcggga aagtccggca gacaatcaag cggaaggccg gtgtccggct tgacctcgaa
    11761 ttgaagatcg taggcgaggc ctgactgaat gatgctgctg gtcaaaggat gatgacggaa
    11821 cgacgacgcc tcacccaggc ccggatcggc gtactgatgg gcggacaatc atccgagcgc
    11881 gaggtgtctt tgcggacggg ggcagcggtg tacggggctc tttgccgctt gggatatgac
    11941 gcggtgaccg ttgacgtcgg cccgacgttg agccgtgact tacaggatca aaagattgcc
    12001 gttgcgttcc tggcgctaca tggtccgggc ggagaggacg gatccattca gggatttttg
    12061 gaaacagtgg gcatccccta cacgggctcc ggcgtgcacg ccagcgccgt gggaatgcac
    12121 aaggtgacca caaaaacggt cctcgcagca catgacattc cggttccggc aggtcttgtt
    12181 atcaagcggg gagagcgcgt cacccatgtg caggctctgc gatcggccaa actacggtgg
    12241 cccgtggttg tgaagcctgc atcagaaggg tcaaccatcg gagtgagtat tgtgaagaag
    12301 cctgctcagt ggaaccctgc cctgtcactc gcccaccgtt acgatcggga tgcgatggtg
    12361 gaagcctaca ttcccggcca tgaagtgacc gtttcgttgc tgggccgaga gaacgagacg
    12421 ccgttggttc ttcccgccgt ggagattgtc gcaccaggcg gcttctacga tttctcagcg
    12481 aagtatgaaa aagggaagac ccaatacctc tgcccggcac ctctttcggc tgccgtcacg
    12541 aaggaaatcc gcgaattggc gttgcgggcg ttcgaagtcc tgggctgcgc aggggccgcc
    12601 cgcgtggatt ttcggattac gcccagggga cgcccgtatg tgctggaact caatacagta
    12661 cccggcatga cggaaacgag cctcttgcca atggccgcgg ttcaggccgg catcggctac
    12721 gaagaattga ccgagcggat tctcgaatcc gcgctcgcaa gaatgaaaaa taaagagcac
    12781 gcggcatgag acttctcacg cgcaagcgcc ccgcaaaggc agccggctat cgcagcaatg
    12841 aatggaaagg tccccgagca gctcagtcaa tggatcaccg gcagcgggcc agacggatcg
    12901 agcggctggc atgctgccga agagccctat gcctctccgg atggctggtg ggtgcaggtg
    12961 ctgtgtgctg gacgatactc atgctttcgc aacagctcgg accggtgctt caccgggggc
    13021 tcgagatcaa acatgtatca gtggaaggga cgcatcaagt caccaaaca
//
LOCUS       DASTQY010000431         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_503633, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000431 DASTQY010000000
VERSION     DASTQY010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503633"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFR82_13900"
     CDS             complement(<1..463)
                     /locus_tag="VFR82_13900"
                     /inference="COORDINATES: protein motif:HMM:NF012915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502743.1"
                     /translation="MKVRIGRDELLTGLQRVQGVVEKRNTMPILSNILIEAKQEGAEI
                     VATDLEIGMRGLYKATVHKAGGITVSARKLYEIIKELPSGDIDLISSENNWTTIQAGK
                     SQFKVVGLPSTDYPALPAIDREGLTPLAGAGLLELIRKTLFAAGDNDARYIL"
     gene            complement(485..1849)
                     /gene="dnaA"
                     /locus_tag="VFR82_13905"
     CDS             complement(485..1849)
                     /gene="dnaA"
                     /locus_tag="VFR82_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU4502744.1"
                     /translation="MGESSGIMTVSTVWQEALAYIQGRVPKQVYDTWFMPVRLERIED
                     RSAQIGVPNKFFGDWLSQHYGPLLEEAVSTARGGGETAVSFVVFSRPPISQSDVAAVA
                     QAVRTATTPRSKRGIQLNPKYSFKNFVVGAGNQFAHAACMAVAEQPAKAYNPLFIYGG
                     VGLGKTHLLNAIANHVAERTDLRIAYLTTEQFTNEVINSIRYDKMMDLRKRYRHIDML
                     MIDDIQFLAGKERTQEEFFHTFNALYEAHKQIVLSSDRFPKDMPDIEERLRSRFEWGL
                     IADLQPPDVETRIAILRKKSEDEGVTLPEDVIQFLSTTMKSNIRELEGSLVRLGAYAS
                     LTGQSITLEMARNVLRDLIGDKKKIVAMEDIQEAVAACFHIKIADLKSRRRSKTLVHP
                     RQIAMYLCRELTDASFPEIGRLFGGKDHTTIMHACRQIMKAKENDSGLNQTLDGLKEK
                     ILRG"
     gene            complement(2160..2390)
                     /locus_tag="VFR82_13910"
     CDS             complement(2160..2390)
                     /locus_tag="VFR82_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502745.1"
                     /translation="MKNTLYLLRKPLDRVDPALFIASHSIGDVVLLDRALVPFPHSGG
                     TVFSLGTEDGDRTISYDVLVKKIFASDGAIVI"
BASE COUNT          520 a          712 c          624 g          614 t
ORIGIN      
        1 tcaggatata gcgggcatcg ttatctcccg ctgcgaacag tgttttgcgg atcaactcca
       61 acaggcccgc cccggcgagg ggcgtcaacc cttcacggtc gatggcggga agcgctggat
      121 aatccgtgct gggaaggccg acgaccttga actggctctt ccctgcttgg atggtcgtcc
      181 agttattttc gctggaaatc agatcaatgt caccgctcgg cagttccttg atgatttcgt
      241 acagctttcg cgccgagacc gtgatcccgc cggctttatg gacggtggcc ttgtagagtc
      301 ctctcatgcc gatttcgagg tcggtggcca cgatctcggc tccttcctgt ttggcttcga
      361 tcaggatatt ggacagaatc ggcatcgtat tgcgcttttc gacgacccct tgaacgcgtt
      421 gaagccctgt gagcaactca tctcttccaa tccgaacctt cataatcgcc cctctctcac
      481 ccggtcaccc tctgaggatc ttctccttga gtccgtccaa cgtttgattc aacccgctgt
      541 cgttctcttt tgctttcatg atttgccggc acgcatgcat aatcgtcgta tggtctttcc
      601 cgccgaacaa ccgcccgatt tccggaaaag acgcgtccgt caattcgcga cacagataca
      661 tggcaatctg cctcggatgg accagcgttt tgctcctgcg ccgtgacttg agatcggcaa
      721 tcttaatatg gaagcaggcg gcgaccgctt cctgtatgtc ctccatggcc acaattttct
      781 tcttgtcgcc gatcaaatcg cgcagcacgt tcctcgccat ctcgagggta atggactgcc
      841 cggtcaatga ggcatatgcg ccgaggcgca ccagcgagcc ttccagttct cgaatgttgc
      901 tcttcatggt cgtggagagg aactggatca catcttccgg cagggtcact ccttcatcct
      961 cggacttctt tctgagaatc gcgattctgg tttccacatc ggggggctgc aggtcggcga
     1021 tgagtcccca ctcaaaccgc gaccgtaagc gttcttcgat gtcaggcatg tccttcggaa
     1081 accggtcgct tgaaaggacg atctgcttgt gcgcctcgta caaggcattg aacgtatgga
     1141 aaaactcttc ctgcgtgcgc tccttccccg ccaaaaattg aatgtcgtcg atcatcaaca
     1201 tgtcgatatg ccggtatcgc ttccgcaggt ccatcatctt gtcgtagcgg atggagttga
     1261 tgacctcatt cgtgaactgc tcggtcgtca gataggcaat gcgcagatcg gtgcgctcgg
     1321 cgacatggtt ggcgatcgcg ttcaacaggt gggtcttccc gagtccgact ccgccataga
     1381 taaagagggg gttgtaggct ttagctggct gttctgcgac tgccatacag gcggcgtgcg
     1441 cgaattgatt gccggcacct acgacgaaat tcttaaacga gtacttcggg ttaagctgta
     1501 tccctcgctt ggatctaggg gtcgtagcgg tccggactgc ttgcgcaact gctgcaacgt
     1561 cgctctggct gattggaggt cggctaaaga cgacaaagga cacggccgtc tctcctccgc
     1621 cccgagcagt cgacacggcc tcctcgagga gtggtccgta atgctggctg agccaatcgc
     1681 caaaaaactt attaggaaca cctatttgcg cacttctgtc ttcaattcgt tctaggcgga
     1741 cgggcataaa ccaggtgtcg tagacctgtt ttggaacccg tccctggatg taggccaagg
     1801 cttcctgcca gactgtagaa actgtcataa ttcctgaact ctcacccata cacaggctta
     1861 ttcacacctg tggataagta tgcttgatgc tctcgcttct tcgaagctca gcgatgccat
     1921 cagccagtgg tggtttgtaa tgtgtctctt catcgacgga tctcaagaag ttatgcacag
     1981 tgcaccgccc tcttactccg agtcaatccc caactccgtc cccaagacgc cctctagaag
     2041 agtacgtccc taatcaacca tttagcacca atccccacca tccaccggtc ctactccgaa
     2101 tcctacgacg gcgactatct ctttctttgc cacactatct taggagaaga gcctcgtttc
     2161 taaatgacga tcgctccatc actcgcgaaa atttttttca ccagaacatc ataggagatg
     2221 gtgcgatccc catcctcagt gcccagtgag aagactgttc cgccggaatg aggaaatgga
     2281 acaagggcac gatcaaggag caccacatct cctatcgagt gtgacgcgat aaacaatgca
     2341 ggatccacac gatccagggg ttttctgagt agatacagcg tgttcttcat gaaaagacca
     2401 gggtgcggtc gagtgagtgc accagcgtgc gaatctcgtc cggcggttta ctcgtcacgg
     2461 aaaatccgtc
//
LOCUS       DASTQY010000432         5350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_418521, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000432 DASTQY010000000
VERSION     DASTQY010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418521"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFR82_13915"
     CDS             <1..238
                     /locus_tag="VFR82_13915"
                     /inference="COORDINATES: protein motif:HMM:NF015835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEU4502746.1"
                     /translation="LLHRLQGGCQVPIAAHATLNGSEIVLDGLVASVDGKEVIRDRVH
                     GTTADPYAAGIQLAERLIGRGAERILQTIYGTPS"
     gene            235..1797
                     /gene="cobA"
                     /locus_tag="VFR82_13920"
     CDS             235..1797
                     /gene="cobA"
                     /locus_tag="VFR82_13920"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEU4502747.1"
                     /translation="MTTGRQGIVYLVGAGPGDPGLLTVKGKECLERADLVLYDYLANP
                     ELLNHAPSAAERIYVGRRGRGQYQDQAEINRLLIERAKAGNVVVRLKGGDPFVFGRGG
                     EEAEAVAAAGVPFEVVPGVTAAIAVPAYAGIPVTHRTLASSLTVVTGHEDPAKGTTVI
                     DWPRLASTSGTLVFMMGMKTLPVIVSRLLEEGRSPDTPVAAVRWGTRADQRTIIGTLR
                     DIVEKTERARLEPPTVIIVGEVVKLREQLNWFEKRPLFGRRIVLTRAPEQAKEFGRLL
                     AAFGAEPIEAPTINIVPPASWEALDRAIARLREYAWVIFTSVNGVAPFMERLGRARQD
                     VRALAHLRIAAIGPRTAEQLTRYGLTADVIPSEFQAEGMLAAMSHHDLSGKKVLIPRA
                     EVAREILPEQLRLKGASVDVIPVYRTMTPSADLSRLKEQLQAGMIDVITFTSSSTVTN
                     FVDMMGGGEETRRLTAKTSVACIGPVTARTAEEQGLTVGILPTESTVPALAHAIVRHY
                     SEGARVAIPISR"
     gene            1831..2223
                     /locus_tag="VFR82_13925"
     CDS             1831..2223
                     /locus_tag="VFR82_13925"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4502748.1"
                     /translation="MVPVKSFMVPREKFVTVERDTSAQAAARIMRDRGIGSLFVTNQK
                     DIIGILTDTDMMRRVVASGSDPNKTTVEQIMSAPILTIEENKTLLDANDLMAQAHVRH
                     LGVTRDSKLVGMISVRDLVVFLTNLPRK"
     gene            2229..3200
                     /gene="hemB"
                     /locus_tag="VFR82_13930"
     CDS             2229..3200
                     /gene="hemB"
                     /locus_tag="VFR82_13930"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEU4502749.1"
                     /translation="MAFPIQRLRRLRQHETFRRMVRETRLSASDLIYPMFVMEGKDQR
                     REIASMPGQFRLSIDLLVKEAGEVAGLGIPAIMLFGIPDRKDDRGTSAVDPNGIVQRA
                     IKAVKDHVPGLIVVTDVCIDEYTSHGHCGIVKDGRILNDETLECLRAMARTHAQAGAD
                     MVAPSDMMDGRVAAIRSELDHAGYPELPIMAYAAKFASCFYAPFRDAAGSAPQFGDRQ
                     SYQMDPANRREALREIALDVEEGADIVMVKPALPYLDVIAAARAHTTLPVAAYQVSGE
                     YSMIKAAGKAGWLDESRAMLESLLAIKRAGADLILTYFAKDAAPFLR"
     gene            3215..4150
                     /locus_tag="VFR82_13935"
     CDS             3215..4150
                     /locus_tag="VFR82_13935"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU4502750.1"
                     /translation="MTRDLGDGRSRPHPVVTIGNFDGHHRGHQSLLQTVVEQARAVKG
                     TAMVLTFDPHPVRVLAPHVQLRFLTSPEEKLARFKAAGIDEVVFLEFTPALAAMTPEE
                     FADIVLHRRLRVSDVYVGEHFAFGRGRAGRIADLERFGRTLGFTVHPVKPVMLDGGVV
                     SSTRIRTFIQAGRMDEASVLLGRAYGITGTVVRGTQKGQALGWPTANLRIPPERVVPP
                     DGVYAARASWECRASDAIAYIGTRPTFGSGERLIEVNLLDQRANLYEKEMSVEFVERL
                     RGDHAFASADELAAQITLDVDRARVSLRRFAEGNR"
     gene            4151..>5350
                     /gene="tilS"
                     /locus_tag="VFR82_13940"
     CDS             4151..>5350
                     /gene="tilS"
                     /locus_tag="VFR82_13940"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU4502751.1"
                     /translation="MAVAATARARHPVFHAMVRTIRDRQLFEPGQHLLVAVSGGPDSV
                     ALLSLLTALAPAWRLTLTVVHFNYGLRGTEADGDEAFVAALCRERGVALVVQRPVLTK
                     RRGAASLQARARVARYRAMAELADDIRADRIVTGHTANDQAETVLLWLLRGAGLTGLS
                     GMRFIRNQRIVRPLLMITRHDVLDYLRREGLHYREDSSNATSLYRRNRIRRELLPVME
                     QIAPSIVRRLERQSHLLRADDEYLEDIVDHLYRSTVIVEPGGNQRFERQAFTRLPAAL
                     QRRLVRRSLRAGDPEGRAPNLRTVESVLRFAAGESKGMLSIRGFDVITECEWIVLQLT
                     PQRTVSSPSGQSFPTVTVPISLPATVYWPGTRQQIHVQVMTKQAAASWLNQRRPECAV
                     FDADRLSA"
BASE COUNT         1036 a         1530 c         1731 g         1053 t
ORIGIN      
        1 gttactgcac cgcttgcagg gaggctgcca ggtgccgatt gcggctcatg ccacactgaa
       61 cggatcggaa atcgtgttgg atggactggt cgcgagcgtg gacggcaagg aggtcattcg
      121 cgaccgtgtg cacgggacga cggctgatcc gtatgcggcg ggcattcagc tggcagagcg
      181 gttgatcggg cgcggagccg agcgaattct tcaaacgatt tacgggacgc cgtcatgact
      241 accggacgtc aggggattgt ttacctcgtc ggcgccgggc cgggtgatcc cggtctgttg
      301 acagtgaaag gcaaggaatg ccttgagcgg gctgatctcg tcctgtacga ttatctcgcg
      361 aatcctgagt tgctgaacca tgcgccatca gcggccgagc gcatttacgt gggacggcgt
      421 ggacgtggtc agtatcagga ccaagcggaa atcaaccggt tgctcatcga acgcgcaaag
      481 gcgggaaacg tggttgtgcg gctgaaaggc ggggatcctt tcgtcttcgg gcgcggggga
      541 gaggaggccg aggcggtagc cgctgccgga gtgcctttcg aagtcgtgcc aggtgtcacc
      601 gcggccattg ccgttccagc ctatgccggc ataccagtta cccatcgtac actcgcgtct
      661 tcactgaccg tcgtcaccgg gcatgaggat ccggccaaag gcacgaccgt cattgactgg
      721 cccaggcttg ccagtacctc gggaacactg gtctttatga tgggcatgaa gacactgccc
      781 gtgatcgttt cgcgtttatt ggaagagggg cggtcgcccg acacgccggt tgccgccgtg
      841 cggtggggca cgagagccga ccagcgaacg atcatcggca cgctgcggga cattgtcgag
      901 aaaacagaac gggcgcggtt agaaccgccg acggtgatca ttgtcggaga ggtggtgaag
      961 ttgcgggagc aattgaactg gttcgagaag cggccgctct tcggaaggcg gatcgtgctc
     1021 acgcgggccc cggagcaagc caaagaattc ggccgtctgc tggccgcctt cggcgcggag
     1081 ccgatcgaag ctcccacgat caacatcgtg ccgcccgcca gttgggaggc gctcgacagg
     1141 gccatcgcga ggctccgaga gtatgcctgg gtgatcttta ccagcgtcaa tggcgtggca
     1201 ccatttatgg agcggctcgg acgcgcccgt caggatgtca gagcgctggc tcatctccgg
     1261 atcgcggcca tcggaccgcg gaccgccgag caactgactc gttatggatt gaccgctgac
     1321 gtcatccctt ccgagtttca agccgaaggc atgttggcag caatgagcca ccacgacctc
     1381 agtgggaaaa aggtgttgat cccgagggca gaggtcgcac gcgagattct gccggaacaa
     1441 ttgcggttga agggcgcgtc ggttgatgtg attccggttt ataggacgat gacgccatct
     1501 gccgacttga gccgtctcaa agaacagctt caggcaggca tgatcgatgt catcacattt
     1561 acgagctcgt cgacggtcac caacttcgtc gacatgatgg gcggcggaga agagacgcgc
     1621 cggctaacgg ccaagaccag cgtcgcgtgc atcggaccgg tgactgcccg cactgcggag
     1681 gagcagggcc tgaccgtcgg aattctgccg acggaaagca ctgtgccggc tctggctcac
     1741 gcgattgtcc ggcactacag cgaaggggca cgagtcgcca ttcccatctc gcgttaggcg
     1801 agttttataa tcagatgagg ggaggttttc atggttccgg tgaaatcgtt catggtccca
     1861 agagaaaagt ttgttacggt cgagcgtgat acgtctgcac aggccgccgc cagaatcatg
     1921 cgcgaccgcg gcatcggcag cttgtttgtt acgaatcaga aagacatcat cggcattctc
     1981 accgacacgg acatgatgcg tcgggtcgtc gcgtcgggca gtgatccgaa caagacgacc
     2041 gtggagcaga tcatgtcggc accgattctc accattgaag agaacaagac gctgttggat
     2101 gcgaacgatc tgatggcgca ggcacatgtg cggcatctgg gtgtgacacg cgacagcaaa
     2161 ctggtcggca tgatttccgt acgtgacctg gtcgtctttt tgacgaatct gccgaggaag
     2221 taacgatcat ggcgtttccg attcaacggc tgagacggct tcgacagcac gagacattcc
     2281 gtcgcatggt gcgggaaact cgtctctcgg cttcggacct gatctatccc atgtttgtca
     2341 tggagggcaa agaccagcgg cgtgagatcg cctcgatgcc cggacagttc agattatcca
     2401 tcgatctgct ggtcaaggaa gcaggggagg tggccggctt aggaattccc gccatcatgt
     2461 tgttcgggat tccggaccgg aaagacgacc gcggcacatc cgcggtggac ccaaacggca
     2521 tcgtgcagcg ggcgattaaa gcggtgaaag atcacgtgcc cggtcttatc gtcgtcaccg
     2581 acgtgtgcat cgacgaatat accagtcacg gccattgcgg tatcgtgaag gacggccgca
     2641 tcctgaacga tgaaacgctg gaatgcctcc gcgcgatggc gcggacgcac gcccaggccg
     2701 gcgccgatat ggtggcgccg tcggatatga tggacgggcg ggtggccgcc atcaggtcgg
     2761 aactggatca tgccggctac ccggaactgc cgatcatggc gtacgcggca aaattcgctt
     2821 cgtgttttta tgctcctttc cgcgatgcgg ccggctccgc gccgcagttc ggcgaccgac
     2881 agtcctatca aatggacccc gccaaccggc gcgaagcgtt gcgagagatt gcgcttgatg
     2941 tggaggaagg agctgatatc gtcatggtaa agcctgcatt gccctatctc gatgtcattg
     3001 ccgccgctcg agcgcacacg acgcttcctg tcgccgccta tcaagtcagc ggcgagtaca
     3061 gcatgatcaa agccgccggg aaagccggtt ggctggacga gtcgcgagcc atgctcgagt
     3121 cgctgctcgc catcaagcgc gccggagcag atctaattct gacctacttc gcgaaagacg
     3181 cggcgccttt tctccgctga cgatgacgtt cacggtgacg cgcgatctcg gcgacggccg
     3241 gtccagacct catccggtgg tcacgatcgg aaactttgat gggcatcatc gcggccacca
     3301 atcactgtta caaacggttg tcgagcaggc acgcgcggtc aaaggcacgg ccatggtcct
     3361 gaccttcgat cctcatccgg tcagggtgct cgcccctcac gtccagctgc ggtttctcac
     3421 gagtccggaa gagaagctcg cccgcttcaa ggcggccggc attgacgaag ttgtctttct
     3481 cgaatttacg cccgccctcg ccgccatgac gccggaagaa tttgcggaca tcgtccttca
     3541 tcgccggcta cgtgtatcgg acgtttatgt cggggaacat tttgccttcg gacgagggcg
     3601 cgccggccga attgcggact tggaacgatt tggccgcacg ctgggattca ccgtccatcc
     3661 cgtcaagccc gtcatgctgg acgggggagt cgtgagttcc acgagaattc gaaccttcat
     3721 ccaagccggt cgcatggacg aggcgtcggt cttgctcgga cgggcttacg gaatcacggg
     3781 aacggtcgtg cgggggacac agaagggaca agcgctcggg tggccaactg ccaatctgcg
     3841 gatccctccg gaacgcgtgg ttccgccgga tggcgtctat gccgcccgag cctcctggga
     3901 atgcagggcg tctgatgcga tcgcgtacat cgggacgaga ccaacgtttg gtagcggcga
     3961 gcgcttgatc gaggtcaatc tcttggacca gcgggcaaac ttgtacgaga aggagatgtc
     4021 cgtagagttt gtcgaacggt tgcggggaga ccacgcgttc gcgtccgccg acgagctggc
     4081 agcccagatt actcttgacg tcgaccgtgc cagggtgagc ctgcgccgat tcgcggaagg
     4141 gaatcgatag atggccgtcg cggcgaccgc acgggcccgg catcccgtgt ttcacgcaat
     4201 ggtccggacc attcgtgatc gacagttgtt cgagccgggg cagcatctcc tcgtcgcggt
     4261 ttccggtggg cccgattcgg tcgccttgtt gtcgctgctg accgctcttg cgccggcgtg
     4321 gcgtctcaca ctcacggtgg tgcacttcaa ttacgggctt cgcgggaccg aagccgacgg
     4381 cgacgaggcc tttgttgctg cgctgtgtcg cgagcggggg gtcgctttgg tcgttcaaag
     4441 accggtgttg accaagcgac gcggggcggc ttcccttcag gcgcgcgccc gcgttgcccg
     4501 ctaccgcgcc atggcggaac tggcggacga catccgcgcc gatcgcattg tcaccggcca
     4561 tacggcgaac gatcaggccg aaaccgtgct cttgtggctg ttgcggggcg caggtctgac
     4621 aggactctcg gggatgcggt tcatccgtaa ccaacggatt gtacgaccgc tgttgatgat
     4681 tacccgacac gacgtgctgg actatctaag acgagagggc ttgcactatc gagaggacag
     4741 cagcaacgcg acgtctcttt atcggcgcaa ccgtattcgt cgcgaactgt tgccggtgat
     4801 ggaacagatc gcgccatcga tcgttcgccg gctggagcgg cagtcgcacc ttctacgagc
     4861 cgacgacgag tatttagaag acatcgtgga tcacttgtac cggtcgacgg tgatcgtcga
     4921 gcccggtggg aatcagcggt ttgaacgaca ggcgtttacc cgccttcctg cggcactgca
     4981 gcgacgtctg gtgcggcgga gtcttcgcgc tggcgacccc gaagggcggg caccgaatct
     5041 gcgtacagtc gaaagcgtgc tgcggttcgc ggccggtgag tccaaaggga tgctttcgat
     5101 ccgagggttt gacgtgatca cagagtgtga gtggatcgtc ctccagttga ctccgcaacg
     5161 aacggtctcc agcccgtccg ggcaatcctt cccgacagtc actgtgccga tctccctccc
     5221 tgctacggtg tattggcctg gtacgcgaca acaaattcat gtacaagtga tgaccaagca
     5281 ggcggccgcg tcgtggttga accagcgaag gccggagtgc gcggtgttcg atgccgaccg
     5341 gttgtccgcg
//
LOCUS       DASTQY010000433         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_676888, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000433 DASTQY010000000
VERSION     DASTQY010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676888"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VFR82_13945"
     CDS             <1..729
                     /locus_tag="VFR82_13945"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU4502752.1"
                     /translation="GSNDPMAVVLTVALIQLMTNPSTNMWDLLWFFLVQMTVGAVAGV
                     AMGELIRWTLNTLKLELRGIYPVLSVAFALLTYGLTAELHGSGFLAVYLAGLFMRRHS
                     VVHQRNLVQFHEGLAWLMQITMFLILGLQVFPARLIPIVGVGLLVSLFLIFIARPVSV
                     YTSLALSRLSFREQTLVAWVGLRGAVPIVLATFPVLAGIQKADTIFHLVFFIALTSVL
                     LQGPPIPWMTRLLGLDRAPQEGRR"
     gene            complement(737..1519)
                     /locus_tag="VFR82_13950"
     CDS             complement(737..1519)
                     /locus_tag="VFR82_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEU4502753.1"
                     /translation="MILPISPFAILILGLVLMLMGFNVLREYERAVIFRWGRLARGLV
                     GGNGPGVVIIIPFIDKLVRVSLRTVTMDVPPQDVITKDNVTVKVNAVIYFRVVDQERA
                     IIQVEDYLYATSMMSQTTLRSVLGQSQLDDLLSKREQINADLQRIIDQQTEPWGVKVT
                     AVEVKNVDLPQEMQRAIARQAEAERERRAKVIHAEGEFEASQRLADAANVISQNPAAL
                     QLRYLQTLVEIAAEKNSTTIFPIPIDTIAPFFKHWTKPADAT"
     gene            complement(1516..>3078)
                     /locus_tag="VFR82_13955"
     CDS             complement(1516..>3078)
                     /locus_tag="VFR82_13955"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEU4502754.1"
                     /translation="RAAAGPIPQPLANSPLHLPRHCHGEQSTTYDTIEATCCSRSGQP
                     AGSPFLDSVKRGSYAPFDCSVSMAFAGGRDTLMPVPGRRPLRFRHLSFIVIALTSLCS
                     PPEASSAEPFVTLAVYDGVINPVSAEYLHDALASAEASGAQALIISLNTPGGLDSSMR
                     LIIKDITGATIPVIVYVSPSSARAASAGVFITMAAHVAAMAPGTNIGAAHPVNMGGGE
                     MDKTMKDKVENDSVAYIKSIAQQRERNAAWGEDAVRKSVSVTEREALKLKVVDLIAEG
                     LPALLQQLDGRRVTLASGPTVLKTAGARVEEFPMGLRLEWLKAISDPNIAYLLMTIGT
                     IGIIAELYSPGAILPGIVGAISLILAFYSFQSLPVNYAGVLLFLLGIVFFVLEASVTS
                     YGLLAIGGVLAMLIGSVMLIKSDAEFFRISWSVILPVVTLASAFSLLSIGMGLKAMRR
                     RPVTGREEMVGLVGIVKTALGTEGQLAVHGELWEAVSEQPLSPGDEAEVTGVDGLRLR
                     VKPYLRKKEVPT"
BASE COUNT          584 a          908 c          943 g          643 t
ORIGIN      
        1 ggcagcaatg acccgatggc cgtagtgttg acggtggcct tgattcaact catgaccaat
       61 cccagtacga atatgtggga cctcctctgg ttctttctcg tgcaaatgac cgtcggggcg
      121 gtggccggcg tggcgatggg cgaactcatc cgctggacgc tcaatacgtt gaaattggag
      181 ttacgcggca tctacccggt gttatcggtg gcctttgcgc tcttgaccta tggcttgacc
      241 gccgagttgc atggcagtgg atttctcgcg gtgtatctgg cgggactgtt catgcgacgg
      301 cactcagtcg tccaccagcg gaacctcgtg cagtttcacg agggtttggc ctggctgatg
      361 caaatcacga tgttcctgat actcggattg caagtctttc ccgcccgcct gattcccatt
      421 gtgggcgtcg gtctcctcgt gtcccttttc ctgattttca tcgcccgtcc ggtcagtgta
      481 tacacgtcac tggcattgtc caggctctcg ttccgcgagc aaacgctggt ggcatgggta
      541 ggtctgcggg gcgcggtgcc gatcgtcttg gcgacgtttc cagtcctggc cggcatccaa
      601 aaggccgaca cgatctttca cctcgtcttt ttcatcgccc tgacgtccgt tttactacaa
      661 ggaccgccga taccttggat gacgcggctg ttggggcttg atcgagcgcc gcaagagggg
      721 cgacgatgag tgccggtcag gtggcgtctg caggcttggt ccaatgcttg aagaacggag
      781 cgatggtgtc gatgggaatc ggaaaaattg tggtggagtt tttctcagca gcgatctcca
      841 cgagcgtctg gaggtagcgg agttgcaacg ccgcaggatt ctggctgatg acgttggccg
      901 catcggccaa ccgttgtgag gcctcgaatt cgccttccgc atggatgacc ttcgcccgcc
      961 gctcgcgttc cgcttccgct tgacgggcaa tcgcccgctg catttcctgc ggcaggtcca
     1021 cgttcttcac ctcgacagcg gtgactttca caccccaggg ctctgtctgt tgatcgatga
     1081 tccgctgcag gtctgcattg atctgctccc gcttggacag caggtcgtcg agctggctct
     1141 gccccaacac gctgcgcagc gtcgtctgcg acatcatcga cgtggcgtac aggtagtctt
     1201 cgacttggat gatggctcgt tcctgatcta cgacacgaaa atagatgacg gcgttgacct
     1261 tgacggtgac gttgtccttc gtaatgacgt cctgcggcgg gacatccatg gtgacggtcc
     1321 gcaaactgac gcggaccagc ttgtcgatga acgggatgat aatgacgaca ccgggaccat
     1381 tcccgcccac gagcccgcgg gccagacggc cccagcggaa gatgacggct cgttcgtact
     1441 ctcgcagcac gttaaagccc atcagcatca gcaccaggcc aaggatgaga atcgcaaaag
     1501 gactgatggg gagaatcatg tggggacctc tttcttcctc aagtaaggtt tgacgcgtag
     1561 tcgcaagccg tcgacgccgg tgacttctgc ttcgtcgcca ggcgacaacg gctgctcgct
     1621 gaccgcttcc cagagttctc cgtggacggc caattgcccc tccgtcccaa gcgccgtctt
     1681 gacgataccg acgaggccga ccatttcctc gcggccggtc accggtcgcc tgcgcatggc
     1741 tttcaagccc atcccgatgc tgagcagtga aaatgccgag gccagcgtca cgaccggcag
     1801 aatgaccgac cacgaaatcc ggaagaactc ggcatcgctc ttgatcagca tcaccgatcc
     1861 aatcagcatc gccaacacgc cgccgatcgc cagcaagccg taactggtca cgctcgcttc
     1921 caacacaaag aagacgatgc cgagcaggaa gagcaggacc ccggcgtaat tgaccgggag
     1981 tgattggaag gaatagaacg cgaggatgag gctgattgcg ccgacgatgc caggaaggat
     2041 cgcccctggg ctgtagagtt ccgcgataat gccgattgtg ccgatggtca tgagcaggta
     2101 agcgatgttc ggatcgctga tcgccttcag ccattcgaga cggagcccca tgggaaactc
     2161 ctctaccctg gctccggctg ttttcagcac cgtcgggccc gacgccagag tgacgcggcg
     2221 gccgtccaat tgctggagca acgccggcag tccttccgcg atgagatcca cgactttgag
     2281 cttcaaggcc tcgcgctccg tgacggacac gcttttgcgt accgcgtctt ccccccaggc
     2341 tgcattgcgt tcccgttgtt gagcgatcga cttaatgtac gcgaccgaat cgttctccac
     2401 cttatccttc atcgtcttgt ccatttcacc gccgcccatg ttcacgggat gcgcggcccc
     2461 gatgttcgtg ccgggagcca tcgcagccac atgggcggcc atggtgatga agacgccggc
     2521 agaagcggca cgtgcgctgg atggtgacac gtagacgatg accggtatgg tcgctccggt
     2581 aatgtcttta atgatcagcc gcatggagct gtccagtccg cctggcgtat tgagcgaaat
     2641 aatcagcgcc tgggcgccgc tcgcttcggc cgaggccagg gcatcgtgca gatattccgc
     2701 cgagacggga ttgatcaccc cgtcgtacac ggcgagtgtc acaaagggct cagcggagct
     2761 tgcctccggc ggggaacaga gggacgtgag ggcgatcacg atgaaagaga ggtggcggaa
     2821 gcgcaacgga cggcgacccg gtaccggcat caacgtgtcc ctcccaccgg cgaaggccat
     2881 ggagacggaa caatcgaagg gagcatacga gccgcgtttt acactgtcaa ggaagggaga
     2941 tccggccggt tggcctgagc gcgagcagca ggtcgcttct atcgtgtcat acgtggtcga
     3001 ctgttcgcca tggcagtggc gggggaggtg gagcgggctg ttcgcgagtg gctgcggaat
     3061 cgggccggcg gcagcccg
//
LOCUS       DASTQY010000434         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1513204, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000434 DASTQY010000000
VERSION     DASTQY010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513204"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VFR82_13960"
     CDS             complement(<1..977)
                     /locus_tag="VFR82_13960"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502755.1"
                     /translation="MWNARLWQFAHPVLIGLALLSIGCQKDSEAIVSIALHPTNSNIL
                     YVATNDAVYKTRDGGQTWEKFPSFSARRVTTVAIDPQLPATVYAGTMGDAVYKSPDGG
                     QHWLPHNVGLKEHVSFVNQFVFHPGLSERIYAATTVGAFYSKDAGREWEERMNGMKEV
                     HIVTSVAIDPRDPSVLYGGTTGGVYRSEDGAMTWKKINNGLIPESELMASMALGVNVI
                     EIDRTNPHVVYAGTTKGLYRTANKGEEWQRIGESIPEPFVSSIVIHPSDSAILYIGGP
                     AGVWKSADGGHTWKATNDGLASLNIRALAMEPKNARTLYAGTNGSGLYRS"
     gene            complement(994..2112)
                     /locus_tag="VFR82_13965"
     CDS             complement(994..2112)
                     /locus_tag="VFR82_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEU4502756.1"
                     /translation="MTSTRRLIFLLGWLAIVLPSIAADTTSFVPAREGYRYAFPRDHG
                     SHDEFRTEWWYYTGNLATNDGRPFGYQLTFFRRGIPLEQVKTLPSRWSITQLYLAHFA
                     VTDLKNGRFHVFEKLSRAGVGKAGAEAERLHVWIDQWRAQSSEAEVGSQTLQASDDDL
                     EIDLTVVPEKAPVIHGKEGVSRKGAESGQASHYYSLTRLATSGTIRLGNESFNVTGTS
                     WMDHEFGSADLGKDLVGWDWFSIQLADHTELMLYRMRRSDGSSDSVSSGTFIDHDGRA
                     QHLTLGEFMLEPLTTWTSPASKARYPSTWRLSIPSKGLSLALVPRMTEQELTTNRSTQ
                     VTYWEGAIEASGTLEGKPIAGRGYMELTGYAERFSKKL"
     gene            2230..>2867
                     /locus_tag="VFR82_13970"
     CDS             2230..>2867
                     /locus_tag="VFR82_13970"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEU4502757.1"
                     /translation="MTILSAIGYRDRMQTDHERDQHEPEPSEKSTPFSIPEHIPVFAL
                     PNVVLFPKTYLPLHIFEPRYRQMVDDAVMGGQCIGIALLKEGWESEYYGNPPVFSTGC
                     VGRLVSVQPLADGRSNILLQGLERFEIIVESYEKPYRQASITLKPRAAEASLDPGVRQ
                     RLVTALEDYLRSRQDAPTWQGWFREDVSDDILVNTLSTYLDCTPLEKQFLLEA"
BASE COUNT          582 a          821 c          811 g          653 t
ORIGIN      
        1 gatcggtaca gcccgcttcc attagttccg gcatagagtg tccgggcgtt tttcggctcc
       61 atcgccagcg ctcggatgtt taatgaagcg agcccgtcgt tggtggcctt ccacgtatga
      121 ccaccatcgg cgctcttcca cacccctgcc ggccctccga tatacaagat cgcagagtca
      181 gaaggatgaa tcacaatgct gctcacgaac ggttctggaa tcgattcccc gatgcgctgc
      241 cactcttctc ccttgtttgc cgtccggtag agacccttgg tggtgccggc atacaccaca
      301 tgaggatttg tccggtcgat ttcaatgacg ttgacgccca aagccatgga ggccatcaat
      361 tcgctctccg gaatgagacc gttgttgatt tttttccagg tcatcgctcc gtcctctgac
      421 cggtatactc ctccggtcgt cccaccgtac agtacggatg gatctcttgg atcaatggcg
      481 accgacgtga cgatatggac ttccttcatg ccattcatcc gctcttccca ttcacgcccg
      541 gcatctttgc tgtagaaagc tcccacggtg gtcgcggcat aaatccgttc gctcagcccc
      601 ggatggaaaa caaattgatt cacaaaggac acgtgctcct ttaatcccac attatgcggc
      661 agccaatgct gccctccgtc gggactcttg tagaccgcat ctcccatcgt gcctgcgtac
      721 accgtggcgg gtagctgcgg atcgatggcc acggtcgtta ctcgtctggc gctaaagctg
      781 ggaaatttct cccaggtttg gccaccgtcg cgcgttttgt acacggcgtc gttggtcgcg
      841 acatacagga tattcgaatt cgtgggatgg agagcgatgg agacaatcgc ttcgctgtct
      901 ttttgacagc cgatgctgag aagcgccaac ccgataagca cgggatgggc gaactgccat
      961 agacgtgcat tccacatctg acgccttttt tgtctacagt ttcttcgaga agcgctccgc
     1021 ataaccggtc agctccatgt agccgcggcc cgcgatgggc tttccttcca aggtgccgga
     1081 cgcctcgatg gcaccctccc aataggtgac ctgtgtgctg cggttcgtgg tcagttcctg
     1141 ctcggtcatc cgaggaacaa gcgcaagcga aagacctttc gacgggatgg acagccgcca
     1201 ggtcgaggga tagcgtgcct tgctggcagg actggtccag gtggtgaggg gctccagcat
     1261 gaactccccg agtgttagat gctgagcccg gccatcgtgg tcgataaaag tgccgcttga
     1321 aacggaatca gatgatccgt cggaccgacg catgcgatag agcatcagtt cagtgtgatc
     1381 ggcaagctgg atgctgaacc aatcccatcc gacgagatct ttgccgagat cggcagaacc
     1441 aaactcatga tccatccaac tggttcccgt cacattgaac gattcattgc ccagcctgat
     1501 tgtccccgag gtcgccagcc tcgttaacga gtagtagtga gacgcttggc ccgactctgc
     1561 gccttttcgg ctgacgccct ccttgccatg aatcacaggt gccttttctg gcacgaccgt
     1621 caagtcgatc tctagatcgt cgtctgaggc ctgaagggtc tgtgacccca cctctgcttc
     1681 ggaggattga gcgcgccatt ggtcgatcca cacgtgcaaa cgttccgcct ccgccccagc
     1741 cttgccgacg ccggcacggc tgagcttttc aaaaacatga aaccgtccgt tcttcaaatc
     1801 ggtcacggcg aagtgagcga ggtagagttg ggtaatggac caccgtgagg gcaaagtttt
     1861 gacttgttcc agcgggatgc cgcgacgaaa aaaggtcagt tgatagccga atgggcgtcc
     1921 gtcgttcgtc gcgaggttgc cggtgtagta ccaccattcg gtgcggaact catcgtgtga
     1981 accgtggtcg cgcgggaatg catatcgata gccttcccgc gcgggcacga aggaggtggt
     2041 gtccgccgcg atggaaggaa ggacgattgc gagccaaccg aggaggaaga tcaatcgccg
     2101 cgttgaagtc atatgtctgc tggtcacgat gcgggcgctt ataacatgcg actgcatgaa
     2161 gtcacaaatc gccagtagcg acaagcgatt tctgcgcttc ttctggtcgt gtgtctcgcg
     2221 gttgcgccgt tgacaatttt gtcagccatc ggctatcgtg accgcatgca aaccgatcac
     2281 gagcgtgatc agcacgaacc ggagccgtcc gaaaagagca cgccattttc cattcctgag
     2341 cacattccag tctttgcgct cccgaacgtg gtcttgttcc cgaaaacgta tctgcccctc
     2401 catatttttg agccgcggta ccgccagatg gtggatgatg ccgtgatggg cgggcaatgt
     2461 atcgggattg cgctgctgaa agaaggctgg gaatccgagt attacgggaa tccgccggtg
     2521 ttctcgaccg gatgtgtcgg tcggctggtc agcgtgcagc cgctggcgga cggccggtcc
     2581 aacattcttc tccagggact cgagcggttt gaaatcatcg tggagtcgta tgagaagccc
     2641 taccgtcaag cttcgattac gctgaagccc agagcggcgg aggcctcgct cgatcctggc
     2701 gtacgacagc gactggtcac ggcgctcgag gactatttgc ggtcccgtca ggatgctccg
     2761 acctggcagg gatggttccg ggaagatgtc agtgacgata tactggtcaa caccttatcc
     2821 acgtacctgg attgcacccc gctggaaaag caattcctgt tggaagc
//
LOCUS       DASTQY010000435         6494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_189272, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000435 DASTQY010000000
VERSION     DASTQY010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189272"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFR82_13975"
     CDS             complement(<1..635)
                     /locus_tag="VFR82_13975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4502758.1"
                     /translation="MMERVLEPELMDDPEQTEAYAAADFAEENQGFVDRFVEYFPDFS
                     GGRVLDVGCGPADIPVRFARRYAACHVLGVDASEPMIRLGQQAVQRAGLGERITLRCE
                     RFEAVAGANVFDAALSNSLLHHLPNPLQFWHKLRQLVKPGSPVLVMDLLRPDSREEAQ
                     AIVDRYAASGPDILRRDFYNSLLAAFTEDEVTSQLARMNLTRLLIDVIDDR"
     gene            complement(653..1144)
                     /locus_tag="VFR82_13980"
     CDS             complement(653..1144)
                     /locus_tag="VFR82_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEU4502759.1"
                     /translation="MGPQGRPPYPHVHQINVSDGGVPKHPVLEAKIDKEGLDGDRQRN
                     LKVHGGPDRALCLFSLELIERLQDEGHSIDSGYSGENLTLAGLEWDHIEPGTQLRIGP
                     EVHLEVTSYCAPCELNAQWFRDRDISRISQKANPGWSRLYARVLQGGVVRPGDKVEIS
                     KRP"
     gene            complement(1146..2003)
                     /locus_tag="VFR82_13985"
     CDS             complement(1146..2003)
                     /locus_tag="VFR82_13985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU4502760.1"
                     /translation="MASDYIDVQICSSVDAGEVMSLLGDSSVQGAWQDERTIHLYWPT
                     TKWSPDRLAQLRQTLRHLTGPGLPEADIVVRTMRHQDWNRQWAESVKPLWIGRRLVVR
                     PSWESVAIGSDQIEIILDPKLAFGTGHHATTRMLLEWLEDVIHGGETVLDVGAGSGLL
                     AMVALRLGADKAVGIDHDPDAIDCAREYASVNGFGPELSLQCGTLTGDRTYDMVLANL
                     DRQTLMMLAEPLAASTGKMLLVSGLLRDQRAEIVEAFARVDLFPDCVREQDGWVAIEF
                     RRAQSCEGA"
     gene            2082..3941
                     /locus_tag="VFR82_13990"
     CDS             2082..3941
                     /locus_tag="VFR82_13990"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HEU4502761.1"
                     /translation="MEFIQYRHQTMAVRVRARQLQQLIGRIERLIARGAAASSRFTTY
                     RLAIFIAGTLSSITLFRSGQYHTGNIVLAAFVILFFIVASYHNRLEQRIHRLRLWALI
                     KSAHLARVQLAWSQVPHKPSDSLPSHLYAHDLDLVGPHSLLHLIDTTVSDHGRTRLTS
                     WLLTQPPDPKVWDTRQRLVKELAPRSLFRDRLVLEANLAGEQEINGRRLAAVLEHPVG
                     FPGLPRTLILQSMLAAGTIVLGVGALLDWLPGYWMLTFGVYALIYLMTDQGEELLEHA
                     VGLHHEMERLSRVLRFLERHAARPDSAMAHTCAPLLEAGRSPSSFTRHIARIFHGISV
                     KAHPLIHLGLNALIPWDLFFAFRLRQFQERVRTRLPLWLDRLAEVEAASALATFAYLH
                     PEYAWPTPAPSVGLASHNLGHPLILSTTRVSNDIALEGLGTIQLITGSNMSGKSTFLR
                     TVGINLCLAQAGAPVCASAFTWSWSRLGCCIRVNDSLDAGLSFFYAEVKRLKTILRAT
                     TDRASPPVLFLIDEIFKGTNNRERLIGSRSYIKALATGNGYGLVSTHDLELTDLEKEI
                     ASLVNAHFQETVSDGALSFDYRLRPGPCPTTNALRIMELEGLPIPRTSSHEAE"
     gene            3951..>6494
                     /locus_tag="VFR82_13995"
     CDS             3951..>6494
                     /locus_tag="VFR82_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4502762.1"
                     /translation="MTHPLRGLLIAQFCGAFNDNAWKLMVALLGIRQLASTMAPGPEF
                     ETASQTQTTLAFVIFTFPLAVVSIAAGLLADRVSKRTVIILMKAVEILLMGAATLALW
                     RNPAGGILPLIVLAGMGVHSALFSPAKYGILAELLPHERLAAGNGQLEMWTFLAILTG
                     TAAPGILLSLTGNAVWMAPLLLTALAFIGFSAAWTIPRVPPARDEGGLANTLQGAWLA
                     LARDRMLRLAICGAVFFWTIASLFAQNILVYAKAVLGVSDWQSGLPLMMLSIGIGIGA
                     RWVGRLSQGRVEYGLIPLGAAGVAVMLLTLGLLAPGLPTTLALMVILGISSALIFVPL
                     NALIQWRAPDDRRGSVIAFENTCVFTGILLGSLGAGALASGGTSTTGIFTAAAAFTVL
                     GTAWAMWLLPEALLRVVLVIVTNSLYRLRIVGHSHVPTAGGALLVPNHISFMDGLLLI
                     ASLDRPVRFVVDAQYAEQPFFKPIMKLFGVIPISPYGGLRVVLRALREAGAAIDRGEL
                     VCIFPEGQITRTGTLLPFRRGFERIMKGRTAPVIPIHLDRVWGSIFSFDRGRFFWKIP
                     ERLPYPVTVSFGRSLPPQSTAHEVRSAIQALGEAAWALRKPDRRPLHREFVHAMRRRP
                     FQFVMSDQSRPHVSAMQALIGSIALARALRTSWEDQKHVGILLPPSVAGALVNVAAPL
                     CGKTSVNLNYTVGKSGLNASVRLAGLRTIVTSRTFVEKAKLELPDTPAVVWLEDLAKT
                     IHRKEKLLAAMLACIAPVRLIERVCGQRVPLSIDAPATIIFSSGSTGEPKGVVLSHYS
                     VDANAQGATQVLHLYRHERVLGILPFFHSFGYLVFWFVMFNNAGLVFHPS"
BASE COUNT         1212 a         2038 c         1849 g         1395 t
ORIGIN      
        1 tggcggtcat caatcacatc gatcagcaag cgcgtcaggt tcatccgggc aagctgggac
       61 gtcacctcgt cttcggtaaa ggcggccagc agcgaattgt aaaagtcccg ccgcaaaatg
      121 tcaggcccgc tggctgcgta tcgatccacg atcgcctgtg cttcctcacg tgagtcaggc
      181 cggagaagat ccatgacgag cacaggtgag ccgggcttca cgagttgacg gagcttgtgc
      241 caaaattgaa gagggttagg cagatgatgc aagagactgt tggagagcgc tgcatcgaac
      301 acattcgctc cggcaacggc ctcgaatcgc tcgcagcgta gcgtgatgcg ctcgccaagc
      361 ccggcccgtt gcacggcctg ttgacccaac cggatcatcg gctccgaggc atccaccccg
      421 aggacatggc aggccgcata gcgtcgggca aatcgtacgg gaatgtcggc cggaccgcag
      481 cccacgtcca atacgcgtcc gccggaaaaa tccggaaaat actcgacgaa ccggtccacg
      541 aaaccttgat tctcctcggc gaagtcggcg gcggcgtagg cttcggtctg ctctggatca
      601 tccatcaact ccggttcgag tacgcgctcc atcatcgtgt tccctcggaa gcctacggcc
      661 tttttgagat ctcgacttta tcgccaggcc gtacgacgcc gccttgcaga accctggcgt
      721 agagacgact ccatcccgga ttcgccttct gggaaatccg gctgatatct cggtcacgaa
      781 accattgtgc attcaattca cagggcgcgc agtagctggt cacttcgagg tgcacctccg
      841 gaccgatgcg gagttgtgtc cctggttcta tgtgatccca ttcgaggccg gccagcgtca
      901 gattttcacc ggaatacccc gagtcgatgg agtggccttc gtcctgcaac cgctcaatca
      961 gctccaaaga aaagagacag agtgcgcggt ccggcccgcc gtggactttc aggttgcgtt
     1021 gacgatcgcc gtccagtccc tccttgtcga tcttcgcctc caagaccgga tgtttcggga
     1081 ctcctccgtc cgagacattg atctgatgga cgtgggggta cggcggccgg ccttgtggac
     1141 ccacctcacg ctccttcaca cgattgcgct cttcggaatt cgatcgccac ccaaccgtct
     1201 tgctctcgaa cacagtcggg aaagagatcg acccgggcaa aggcttcgac gatttcagca
     1261 cgctggtcgc gtaacaggcc cgatacgaga agcatcttgc cggtactggc cgcgagcggt
     1321 tcagccaaca tcattagggt ctgccggtcg agattggcca ggaccatgtc ataggttcga
     1381 tcgcccgtaa gggtcccgca ttgcagagac aattccggtc cgaagccatt gacggaggca
     1441 tattcgcgtg cacagtcaat cgcgtccggg tcgtggtcga ttcccacggc cttatcggct
     1501 ccgagtcgga gtgcgaccat ggctaagagg ccgctgccgg ccccgacatc gagcacggtc
     1561 tcgccgccgt gaattacgtc ctcaagccat tccagcaaca tgcgcgtcgt ggcatgatgg
     1621 ccggtgccga acgccagttt gggatcgagg atgatttcga tctgatcgga accgattgcc
     1681 acggactccc agcttggtct gacgacgagc ctcctgccga tccacaaagg cttgaccgac
     1741 tcagcccatt gccggttcca gtcctgatgc cgcatggtgc gcacgacgat gtccgcctca
     1801 ggcaagccgg gcccggtcag atgacgaagg gtctgcctga gttgtgccaa acggtcggga
     1861 gaccatttgg tggtcggcca atacagatga atcgtgcgct catcctgcca ggcgccttga
     1921 acagacgagt cacccagcaa gctcatgact tcgccggcat ccacgctgga gcagatttga
     1981 acatctatgt aatcggatgc catacggatt ctctataggc cattcacaag ctgtcgtgac
     2041 catgaaaccg gttgttcaac gacgggttcg tcattgacgc catggaattc atccagtatc
     2101 gtcaccagac aatggcggta cgcgtgcgcg cccggcaact tcagcagctg atcggcagaa
     2161 tcgaacggtt gatcgcgcgc ggggcagccg ccagctcccg tttcacgacc taccgtctgg
     2221 ccatcttcat tgcgggaacg ctcagcagca tcacgctgtt ccggtccggc caataccaca
     2281 ccggcaacat cgtcctggcc gcattcgtca tcctcttctt cattgtcgcg tcgtatcata
     2341 acaggcttga acagcggatt catcggctgc gcttgtgggc gctgattaaa tctgcgcatc
     2401 tcgcgcgtgt ccaactcgca tggtcccagg ttcctcacaa gccttctgat tcgctgccgt
     2461 cgcatctcta tgcccacgac ttggatttgg tgggaccgca ctcactgctg catctgatcg
     2521 atactaccgt ttcggatcac ggccgaaccc gcctgacatc gtggctcctg actcaaccac
     2581 ctgacccgaa agtgtgggac acccgccaac gcctcgtcaa agaactcgct ccacgttcgc
     2641 tgttccgtga ccggttggtc ctcgaagcca acctggcagg tgagcaagag atcaacggtc
     2701 gccgattggc agcggtgctc gagcatccag tcggctttcc cggattgccg cgcaccctga
     2761 ttctccaatc gatgttggcg gcaggcacga tcgtgcttgg agtcggcgcc ttgttggatt
     2821 ggctgccagg ttattggatg ttgacgttcg gcgtctacgc cttgatctat ctcatgaccg
     2881 accagggcga agagctactc gaacacgccg tcgggctgca ccacgagatg gaacggctga
     2941 gccgggtcct gcgctttctc gaacgacatg ccgcccggcc tgactcggcc atggcacata
     3001 cctgcgcccc tctgctcgag gcgggtcgtt cgccctcctc gttcacacgg catattgccc
     3061 gcatcttcca tggaatcagc gtgaaggcac atcccttgat ccatcttggg cttaatgcgc
     3121 tcatcccgtg ggacctgttc ttcgcgtttc gcctgagaca atttcaagaa cgtgtgcgca
     3181 cacggcttcc tctgtggctc gatcgattag ctgaagtgga agcggcttcg gccctcgcca
     3241 cctttgccta tctccacccc gagtacgcct ggccgacccc ggccccgtcc gtgggtctcg
     3301 ccagccacaa cttaggccac cctttgatcc tctccacgac gcgcgttagc aacgacattg
     3361 ccttggaagg gctcggcacg attcagttga ttacgggttc gaacatgtcg ggcaaaagca
     3421 cctttcttcg aacagtcggc atcaatctct gcttagcgca ggccggagcc ccggtctgcg
     3481 cctcggcctt tacctggagc tggagccgcc tgggctgctg catccgcgtg aacgactcgc
     3541 ttgatgcggg tctgtcgttc ttctatgcgg aagtcaaacg cctgaagacc atcctgcgcg
     3601 ccacgaccga tcgggcttcg ccaccggtcc tgtttctcat cgacgaaatt ttcaagggaa
     3661 cgaacaatcg cgagcgcttg attggcagcc gctcatacat taaggccctg gcaaccggaa
     3721 acggctacgg gctggtcagc acgcacgatc tggagctcac ggacctcgag aaggagatcg
     3781 cctcgctcgt gaacgcacac tttcaggaaa ccgtctctga cggtgcactg tccttcgatt
     3841 accgcctccg tcccggccct tgcccaacga cgaacgccct gcggatcatg gaattggaag
     3901 ggttgcctat tcccaggacc tcctcacatg aggcagaata gccctcacga atgactcatc
     3961 ctctgcgcgg attgctcatc gcccaattct gcggcgcgtt taacgacaat gcgtggaagc
     4021 tgatggttgc gctgctgggc atccgtcagc tggcttccac gatggccccc ggccctgagt
     4081 tcgaaacggc ctcgcaaacc caaaccacgt tagcctttgt catcttcacc tttccgctcg
     4141 ctgtcgtgtc aatcgcggcc ggcttgctgg ccgaccgggt cagcaagcgc accgtgatca
     4201 tcctgatgaa ggcggtcgag atcctcttga tgggtgccgc cacgctcgcc ttatggagga
     4261 atcccgccgg aggaattctg ccgctgatcg tgctcgcagg catgggcgtg cacagtgccc
     4321 tcttcagtcc ggccaaatac gggattcttg cagaactatt gccgcacgaa cgattggcgg
     4381 ccggcaatgg acagctcgag atgtggacgt tcctcgccat tttgaccggc accgcggcac
     4441 cgggcattct gctgtcgctg accggaaacg ccgtctggat ggccccgctg ttgctcacgg
     4501 ccttggcgtt catcggattc agtgcggcct ggaccattcc acgcgtgccg ccggcgagag
     4561 acgaaggcgg gcttgccaat acattgcagg gggcatggtt ggcgctggcg cgtgaccgca
     4621 tgttgcggtt agccatctgc ggcgccgtgt tcttctggac aattgccagt ctctttgccc
     4681 agaacatcct cgtgtatgcc aaggccgtgc tgggcgtctc ggattggcag tccggactgc
     4741 cgctcatgat gttgtcgatc ggcatcggca tcggagcccg atgggtcgga cgtctctccc
     4801 agggacgggt ggagtatggc ctgattccct tgggggcagc cggcgtggca gtcatgctct
     4861 taacacttgg ccttctcgct ccgggtctgc cgaccacgct ggcgctgatg gtgatcctcg
     4921 ggatctcgag cgcattgatt ttcgtcccgc tgaacgccct gattcaatgg cgcgcaccgg
     4981 atgaccgacg tggatcggtc atcgccttcg agaacacgtg cgtgtttacc ggcatactcc
     5041 tgggatcgct tggagcaggg gcgcttgcca gtggcggaac gtcaacgaca ggaattttca
     5101 cggctgcggc cgcgttcacc gtccttggca cagcctgggc gatgtggctg ctccccgagg
     5161 cgttgctgcg ggtcgtcctc gtcattgtca ccaacagctt gtatcggctc cgcattgtcg
     5221 gccacagtca tgtaccgacc gcaggcggtg cgctgctcgt gcctaatcat atctccttca
     5281 tggacggcct gctcctgatc gccagcctcg atcgacctgt gcgatttgtc gtggacgcac
     5341 aatatgccga acagccgttt ttcaaaccga tcatgaagct gttcggcgtc attcccattt
     5401 ctccctacgg cgggcttcgc gtcgtgctgc gcgcgttgcg agaagccggt gccgccatcg
     5461 accggggaga gctggtctgc attttccccg agggacaaat aacgagaacg ggcactctgt
     5521 tgccgttccg ccggggcttc gagcggatca tgaaaggcag aaccgcgccc gtcattccga
     5581 tccatctgga tcgcgtctgg ggcagcatct tcagtttcga tcggggccgg ttcttctgga
     5641 aaatcccgga acgcctgccg tatcccgtca cggtgtcctt tggcagatcc ctgcctccac
     5701 agtcgacggc ccacgaggtt cgcagcgcga ttcaggcgct gggagaggcc gcttgggcgc
     5761 tgcgcaaacc ggatcgacgc ccactgcatc gcgaattcgt tcacgcgatg agaagacgcc
     5821 cgttccagtt tgtcatgtcg gatcagagcc ggccgcatgt gtcagctatg caggcgttga
     5881 tcggatccat tgccctggcc cgagcattgc ggacgtcctg ggaagaccag aaacatgtcg
     5941 gcattctcct tccaccgagc gtcgccggcg cactggtcaa cgtcgcggcg cctctgtgcg
     6001 gaaaaaccag cgtcaatctc aattacacgg tgggcaagtc ggggctcaac gcctccgtcc
     6061 gccttgccgg ccttcgcacc atcgttacca gccggacctt cgtcgaaaaa gccaaactcg
     6121 aattgcctga tacaccagca gtcgtatggc tggaagatct ggctaagacg atccatcgaa
     6181 aagagaagct tctcgcagcc atgctcgcat gcatcgcgcc ggttcgcctg atcgagcggg
     6241 tttgtggcca gcgcgtacca ctttccatcg atgctccggc gaccattatc ttcagcagcg
     6301 gcagtacggg cgagccgaaa ggcgtcgtgc tgtcgcatta cagcgtggac gccaatgcac
     6361 agggcgcgac gcaggtcctc catctctata gacatgaacg agtccttgga atcctgccgt
     6421 tctttcattc gttcggatat ttggttttct ggttcgtgat gttcaacaac gccggactgg
     6481 tctttcatcc ctct
//
LOCUS       DASTQY010000436         9526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1755623, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000436 DASTQY010000000
VERSION     DASTQY010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9526
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755623"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..791)
                     /locus_tag="VFR82_14000"
     CDS             complement(54..791)
                     /locus_tag="VFR82_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502763.1"
                     /translation="MACLCLLPASLSAAPYFEDGFLGLTQKELHEKMGMPQAVRDRKS
                     ALRVFSYYAITDWDKYFRKLVSPENGEDVYTFKRDGIDVRYSFSYAVDPNEDSENRTL
                     YVRLVDIEFSPAVPISQVPSLVPEFRPSTDPASPSFRSNIWILLFKGTASPQARFIVK
                     EAAKDSLDWTLAYQLFSLQGLPDPLTTKSAVDRLEISTQSIDLVKRRQKHTHDPIMNP
                     YSSEFSQQQPPPRPAQAKKIPVPKYAD"
     gene            941..4663
                     /gene="metH"
                     /locus_tag="VFR82_14005"
     CDS             941..4663
                     /gene="metH"
                     /locus_tag="VFR82_14005"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEU4502764.1"
                     /translation="MMTRFGFVIGKERDVTPVELLLKERILILDGAMGTMIQQRKLDE
                     TAFRGERFRDWKKDLKGHNDLLNLTQPAIIEDIHRQYLEAGADIVETNTFNSQAISLS
                     DYQMPQLAYELSRSGAECARRAVVQVQTAQPERRCFVAGAIGPTTKTSSISTDVNNPA
                     ARGATYDELVSAYADQVRGLLDGGADLLLVETIFDTLNAKAAFFAIQQLFAAGAKRVP
                     IMASVTFIQAGSDRGVTGQTVEAFWNSISHVPLLSVGMNCALGPKEMRPLIEELSRIV
                     PLYVSAHPNAGLPNPLLPTGFPETPDTLAPQLREWAQNGWLNIVGGCCGTTPAHIKLL
                     VESVRGIQPRPIPTVEPYTRLSGLEALTIRPDANFVNIGERTNVTGSPAFAKLILQGN
                     YESALAVARQQVEGGAQIIDVNMDEGMLDSKAAMERFLRLIASEPDICKVPIMVDSSK
                     WEVLETGLKNIQGKAVVNSISLKEGIPKFIEQATLVHRYGAAVVVMAFDEKGQADTLE
                     RKIEICTRSYKILTEQVGFKPEDIIFDPNILTVATGIEEHNNYAINFIEATRWIKQHL
                     PGAKVSGGISNISFSFRGNNVVREAMHAAFLYHAIQAGLDMGIVNAGQLAVYEEIPKD
                     LLELVEDVLFNRRPDATERLVTFAETVKRQDKAVVKDDEWRKGTVEERLSHALVKGIT
                     DYIDQDTEEARLKYPKPLSVIEGPLMAGMNIVGDLFGSGKMFLPQVVKSARVMKKAVA
                     YLLPFMEEEKRRVGGYRAQGKVLLATVKGDVHDIGKNIVGVVLGCNNYEVIDLGVMVS
                     CEHILAAAREQKADIVGLSGLITPSLDEMVHVAKEMTREGFDVPLLIGGATTSKAHTA
                     VKIAPSYEPSVIHVLDASRAVGVVSSLMNREQKTSFVQKVRQDYDRVRQAHLTREVRP
                     VLPLAEARARHLTSDWRALHIPEPEFLGIRVIPDQPLVDLVPFIDWSPFFHTWELNGR
                     YPGIFDHPTIGARAKELFTDAQGLLARIISEKLLLAKGVYGIFTASCIGDDIELYTDA
                     SKSAVMTTIHTLRQQAVKPDGQANLALADYVAPKESGRTDHVGAFAVTSGINLEELCS
                     QFDRDHDDYNSIMAKALADRFAEAFAEFLHKRVRSEWGYGRSENLSNDDLIHEKYRGI
                     RPAPGYPACPDHTEKRLLFDLLRVESNAGITLTESFAMLPAAAVSGWYFAHPDARYFA
                     VGKIGRDQVEDYARRKEMELHIVERWLSPNLNYEPA"
     gene            complement(4674..5183)
                     /locus_tag="VFR82_14010"
     CDS             complement(4674..5183)
                     /locus_tag="VFR82_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502765.1"
                     /translation="MAKKSLCVKKAGITRNWIAYLCEALVTSGVMPTWEAATYLTENL
                     FGESPNPRLLGTSNAYEVTSQFLHRLYQPLAEAASREVSLPHGAMVHVFVDISLTGKS
                     AVLVVYFPEGGQPHQLLMLDAAKAWDLVFQDSEAFNRWAEDRFRLLKSALTAAIGKPA
                     RQPLLAEVS"
     gene            complement(5351..6493)
                     /locus_tag="VFR82_14015"
     CDS             complement(5351..6493)
                     /locus_tag="VFR82_14015"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU4502766.1"
                     /translation="MTLLLDRPTRQALLDLKRVLIIDDEEPIRRLLGYMLQSHGYETV
                     LAADAREARLRLDETPFALVLCDVNMPGESGMDLVRNIFRQYPHTAAIMVTGLDSPLL
                     ANAALEMGAFGYIIKPFESNEVLINVANALRRRRLEMENRLHRENLEDVVRTRTMALQ
                     QALEWLERSEKELRLSREETIQRLAIAAEYRDSATAQHIQRMSHYCELLARRYGLSTE
                     RCDLIRTASPMHDIGKIGTPDHVLLKPGKFTQDEFNVIAQHAEIGYRILAGSDAEILN
                     VAATIALTHHERWDGSGYPRKLKGEAIPIEGRIASIADAFDALTTARVYKPAFGFDHA
                     VDLMSKHRGEHFDPELLDVFLASLGDIKRIHEQYADRLTPGLPRQL"
     gene            6878..7621
                     /gene="rph"
                     /locus_tag="VFR82_14020"
     CDS             6878..7621
                     /gene="rph"
                     /locus_tag="VFR82_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEU4502767.1"
                     /translation="MNPGASLVRADGRRREQIRPVKVTRNFIKHAEGSVLIEMGDTKV
                     ICTASIEEKVPPFLKGKGTGWVTAEYSMLPRATHDRSPREAVKGKQGGRTLEIQRLVG
                     RALRAVTDMAQLGERSIWIDCDVIQADGGTRTASITGAFVALADAMAILKTRGVIKKR
                     PLNDYLAAISVGKVGGEILVDLAYTEDCIAEVDLNLVMTGKGQFVEIQGTAEKTPFAK
                     QDMDAFLSQGWGAIQTLTALQRDLIGALE"
     gene            7625..8239
                     /locus_tag="VFR82_14025"
     CDS             7625..8239
                     /locus_tag="VFR82_14025"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HEU4502768.1"
                     /translation="MARELVLATRNRHKGEELAALLGGLDIRIHTMDEFPDVPDVIED
                     GETCEANAIKKAQSISKATGLLAVADDTGLEVDALDGRPGVYAARYAGENATYEDNWK
                     KLLGELTGVPRDRRTARFVTVAAIVSPGETVQVVTGHLDGLIAEVPAGAQGFGYDPVF
                     FVPQLGRTLAEMSSVEKNRISHRAKAFAQVRNLLQNSLLRSVGA"
     gene            8230..8306
                     /locus_tag="VFR82_14030"
     tRNA            8230..8306
                     /locus_tag="VFR82_14030"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8264..8266,aa:Pro,seq:tgg)
     gene            8622..9020
                     /locus_tag="VFR82_14035"
     CDS             8622..9020
                     /locus_tag="VFR82_14035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4502769.1"
                     /translation="MLPDNKVRVLIVDDHFIVRQTFRCILKHYPNIEVVGEASDGDEA
                     IACAGRFQPSVVLMDMNMKKMDGITAARLIKTQYPHVLVLGFSADTQNYNVYAMQQAG
                     AFEVLQKEDAMKDLYAAIQRGVAAVQSNVA"
BASE COUNT         2145 a         2546 c         2758 g         2077 t
ORIGIN      
        1 tctcacaagc ccagtcccag taatcgcagt cacggccttc ctcacgcaag tcttcagtcc
       61 gcatacttcg gaacggggat ttttttggct tgagccggcc gcggaggagg ctgctgttgg
      121 gagaactcag atgaataggg attcatgatc gggtcgtggg tgtgtttttg ccggcgtttc
      181 acgagatcga tgctttgcgt gctgatttcg aggcggtcaa ccgccgactt tgtcgtcaaa
      241 gggtcgggaa gaccttgcag ggaaaacaac tgatacgcga gcgtccagtc gagcgagtcc
      301 ttggcagctt ccttgacgat aaaccgtgcc tgcggcgagg cggtgccttt aaaaagaagg
      361 atccagatat tcgatcggaa tgaaggcgat gccggatcag tagaaggacg gaattcggga
      421 acaagagacg gaacctggct gatgggtacg gctggcgaga attcgatgtc caccaggcga
      481 acatataatg tccggttttc gctgtcctca ttggggtcca ccgcataact gaaggaatag
      541 cgcacgtcga tcccatcccg cttgaaggtg tacacgtcct cgccgttctc cggtgaaacc
      601 aactttctga aatatttatc ccagtcggtg atcgcgtagt aactgaagac acgcagagcg
      661 gatttgcggt cgcggacagc ctgaggcatg cccatcttct catgcaattc cttctgcgtg
      721 agccccagga aaccgtcttc gaaatagggg gccgcggata gcgaagcagg gagcaggcag
      781 aggcaggcca tgagcccgaa agcgcacgtg gcgaagcgtt gtacatggtt ccgcaggagc
      841 aaagcgcctc gtctccgacc gatgtgccat cgtatcggcg gccccgggcc ctgtcaaggt
      901 ggagttgtcc aattggttgc cgggttgaac ctgaatcagt atgatgacac gattcgggtt
      961 tgtcataggg aaggaacgcg acgtgacgcc tgttgagcta ctccttaaag aacgcatcct
     1021 gattctggat ggagccatgg gaacgatgat ccagcagcgg aagctggacg agaccgcctt
     1081 tagaggggaa cggttccgtg actggaagaa agacttgaaa gggcacaatg atctgctcaa
     1141 tttgacgcag ccggcgatca tcgaagatat ccatcgtcag tatctggaag cgggcgccga
     1201 tatcgtcgaa acgaacacat ttaactccca ggcgatctcc ctttcggatt accagatgcc
     1261 acagttggcc tatgaactct caaggtccgg tgccgaatgt gcccggcggg ccgtagtcca
     1321 agtccagact gctcaaccgg agcgacggtg ctttgtggcc ggtgcgattg gaccgactac
     1381 caagacatct tccatttcga cggacgtcaa caacccggcg gcgcgtggag cgacctacga
     1441 cgaactggtg tccgcctatg ccgaccaggt gcgaggtttg ttggacggcg gggccgacct
     1501 gttgttggtg gagaccatct tcgacacact gaatgcaaag gcagccttct ttgccattca
     1561 gcaactcttt gcggccgggg ccaaacgcgt accgatcatg gcctcggtga cattcattca
     1621 agcggggagc gatcgtggtg tgacggggca gacggtggag gcgttttgga actctatctc
     1681 acacgtgccg ctcctgagtg tcgggatgaa ctgcgccctg ggtccaaagg agatgcgccc
     1741 cctcattgag gagctgtcac gaatcgtgcc cctctatgtc agcgcccatc cgaatgccgg
     1801 tctgccgaat ccgctgcttc cgacgggctt tcccgagacg cccgatacgt tggcgccgca
     1861 actgcgggaa tgggcgcaga acgggtggct gaacattgtc ggtggatgct gcgggaccac
     1921 acccgctcac atcaagctcc tggtcgaatc ggttcgcggg atccagccgc gcccaattcc
     1981 gacagtggag ccgtataccc gcttgagcgg tctggaggca ctcacaatcc gtccggatgc
     2041 caacttcgtg aatatcggag agcggacgaa tgtcaccgga tcgccggcgt ttgccaagct
     2101 cattctccag ggcaattacg aatcggcctt ggcggtggcc cgacaacaag tagagggagg
     2161 cgcacaaatc atcgacgtca acatggatga gggtatgctg gattcgaagg ccgccatgga
     2221 gcggtttttg agactgatcg cctccgaacc ggatatctgc aaagttccga tcatggtcga
     2281 cagctcgaag tgggaggtct tggagaccgg cctcaaaaac atccagggca aggcggtcgt
     2341 caacagcatc agtttgaaag aaggcatccc gaagttcatc gaacaggcga cgttggtcca
     2401 ccggtatgga gccgccgtcg tcgtgatggc attcgatgaa aaaggacagg ccgataccct
     2461 ggaacgcaaa atcgaaattt gcaccagatc ctacaagatc ctgacggaac aagttggttt
     2521 caaaccggaa gacattattt tcgatcccaa tattctgact gtcgccaccg gcattgagga
     2581 gcacaacaac tacgcgatca atttcatcga ggccacgagg tggattaaac agcatctgcc
     2641 aggagcgaag gtcagcgggg gcatcagcaa tatctcgttt tccttccgag gaaataacgt
     2701 ggtccgcgaa gccatgcacg cggcttttct ctaccatgcc atccaggcag gcctcgatat
     2761 gggcatcgtg aatgccggtc aattagccgt ctatgaggag ataccgaagg atttactcga
     2821 acttgtagag gacgtccttt tcaatcgccg tccggacgcc accgaacgtc tcgtgacctt
     2881 tgcggaaacg gtcaagcgtc aggacaaggc cgtcgtgaag gatgacgagt ggcgcaaggg
     2941 gacggtcgaa gaacgcttgt cgcatgccct ggtgaaggga atcaccgact acattgacca
     3001 ggacacagaa gaggctcgcc tgaaatatcc gaagcccttg tcggtgatcg aaggtccgct
     3061 catggccggc atgaacatcg tgggcgatct cttcggttcc ggcaaaatgt tcctcccgca
     3121 agtggttaaa agcgcccgcg tgatgaaaaa ggcggtcgcc tatctgctgc cgttcatgga
     3181 ggaggagaag cggcgggttg gtggatatcg cgcgcaaggc aaggtcctcc tggctacggt
     3241 caagggagat gtccacgaca ttggaaagaa catcgtcggg gtagtcctgg ggtgcaacaa
     3301 ttatgaggtg attgatctcg gcgtcatggt gtcatgcgaa cacattctgg cggcggcccg
     3361 tgagcaaaag gcggacattg tcgggctcag cggccttatt acaccgtcgc tggacgaaat
     3421 ggtgcatgtg gccaaggaga tgacgcgcga aggcttcgat gtgccgctcc tcattggggg
     3481 ggcgaccacc agtaaagccc acactgcggt caaaatcgcg ccgtcctatg agccgtcggt
     3541 cattcatgtg ctcgatgcat ctcgcgcagt cggcgtcgtc agcagtctca tgaatcgcga
     3601 gcagaaaacg tcgttcgtgc aaaaggtgcg acaggactac gatcgtgtgc ggcaggctca
     3661 tctgaccagg gaagtcagac ctgtgctccc gctcgcagag gcacgcgcgc gccacctcac
     3721 gtccgattgg cgggcacttc atatccctga gccggagttc ctcggcattc gggtaattcc
     3781 cgatcaaccg ctcgtcgatc tcgtgccgtt cattgattgg tctccgtttt ttcatacatg
     3841 ggaactcaat ggacgctatc cgggcatctt cgatcatccg acgatcggag cgagagccaa
     3901 ggagctcttt accgacgcac agggcctatt ggcacgaatt atctcggaga aattgttgct
     3961 agccaaaggt gtctacggca tcttcaccgc ctcgtgcata ggtgacgata ttgaattgta
     4021 taccgacgcc tcgaagagtg ccgtcatgac gaccatccat acattaagac agcaggcggt
     4081 caaacctgac gggcaggcaa acctcgcact ggctgattat gtcgctccga aagaatccgg
     4141 acgaaccgac cacgtcgggg cctttgccgt cacgtcggga atcaacctgg aagagctgtg
     4201 cagccagttt gaccgggacc atgacgacta caactcgatc atggcaaagg cgctggccga
     4261 ccggttcgcc gaagcgttcg ccgagttcct ccataagcgg gtgagatccg aatggggata
     4321 tggccgatcg gagaatctgt cgaacgatga tctgattcac gaaaaatatc gggggattcg
     4381 ccccgcgccc gggtatccgg cgtgccctga ccataccgag aagcggttgt tgttcgacct
     4441 tcttcgtgtc gaatccaatg ccggcataac gttgacagaa agcttcgcca tgctaccggc
     4501 cgcggcggta agcggatggt atttcgcgca tccggacgcg cgctattttg cggtaggtaa
     4561 aatcggcagg gaccaggttg aggactacgc gcgtcggaag gagatggagc tccacatagt
     4621 ggagcgctgg ctgtcgccga accttaacta cgaacccgcg tgatgcttgc cggttaagag
     4681 acttccgcga ggagcggctg cctggccggt ttgccaatgg ccgcggtaag agccgatttg
     4741 agcagtctga atcgatcctc cgcccaacgg ttgaaggcct cagagtcctg aaagaccaga
     4801 tcccaggcct tcgcggcatc cagcatcagc aactggtgtg gttggccgcc ttccgggaaa
     4861 tacacgacca ggacggcgga ctttcctgtc aaactgatat ccacgaagac atgcaccatt
     4921 gcgccgtgcg ggagtgagac ttcacgtgaa gccgcttcgg ccagaggctg gtataagcgg
     4981 tgaaggaact gtgaagtgac ttcataggca ttgctggttc ccagcaggcg cgggttcgga
     5041 gattctccga agaggttttc ggtcagataa gttgccgcct cccacgtcgg catgaccccc
     5101 gacgttacga gtgcttcaca aaggtatgca atccagtttc ttgtgatacc cgctttcttc
     5161 acacacaggg attttttggc catgagcggc aatcctttcg atgccaacgt gacggcgtgt
     5221 ctaacgtgcc tcttttcaga gaacgaactt gcaactcatt ggcagtatag ctgcgaccgg
     5281 accacagtca attaaatgca ctaaactgac gaagcggtgc cgagacttga acgaggggag
     5341 cgaggtgaat ttacaactgg cgtggaaggc ctggcgtaag gcggtccgcg tactgttcgt
     5401 gaatgcgttt aatatcgcca agtgaggcga gaaagacgtc gagcaactca ggatcgaaat
     5461 gctcgccacg gtgtttgctc atcaagtcca cggcgtgatc gaatccgaac gccggtttgt
     5521 acacgcgagc ggtagtgagg gcgtcgaagg cgtcagcaat cgaggcgatt cggccttcga
     5581 tcggaatagc ctctcctttc aacttccgtg gatacccact tccatcccat cgctcgtgat
     5641 gcgtcagcgc aatcgtcgcc gctacgttca aaatctcagc gtcggatccg gccaggatgc
     5701 ggtagccgat ttccgcatgt tgggcaatca cgttgaactc gtcctgtgta aacttgccgg
     5761 gtttgagcaa cacgtgatcg ggcgtgccga ttttcccaat gtcgtgcatg gggctggcag
     5821 tgcggatcaa gtcacagcgc tcggtggaca atccgtagcg gcgtgcgagc agttcacaat
     5881 aatgactcat gcgttggata tgctgggcgg tggcgctgtc tcgatattct gccgcgatgg
     5941 cgagccgctg gatggtttct tctcgcgaca gccgtaactc cttctcgctc cgctccagcc
     6001 attccaacgc ctgttgcagt gccatcgtcc tggttctgac gacgtcctcg agattttcgc
     6061 gatgcagacg gttctccatt tcgagacgcc gacggcgtag agcattcgct acgttgataa
     6121 ggacttcgtt ggattcgaat ggtttgatga tgtagccgaa tgcccccatc tccaatgcag
     6181 cgttggcaag aagcggactg tccagccccg tcaccataat cgctgccgtg tgaggatact
     6241 gacgaaagat gttccggact agatccatgc cggattctcc cggcatgttg acatcgcaca
     6301 gcaccagcgc aaacggtgtt tcatccagcc gtaagcgggc ctctctggca tcagcggcca
     6361 agaccgtctc ataaccgtgc gattggagca tgtacccaag caggcgccgg atcggctctt
     6421 cgtcgtcgat gatcaacact cgcttgagat cgagcagcgc ttgtcgggtg gggcgatcga
     6481 ggagcaacgt catggctcga tgatatctcg ggtcaatgag gtcgttgtgt cgtcggcatg
     6541 ggtttcgatg gtcccgttga gaactctaga acgaggtgca ccttttgtgc cactaattag
     6601 tgattttgca ccgacttagg cggcagtttt ttcgtatcga ttttctgacc ccacacccgc
     6661 gaaaacgtgt gagttcgtca tccgaactag tgatagcatc cgtgcgaagc ctgctggtcc
     6721 accatccagc ctctgtacaa tctcgtggcc accggccaaa ttctgtacgg taaggcatac
     6781 gtattttcct ctgtcttcca gtgcagatcg catagccgtt tcattgcgat ttggaaatcg
     6841 cttcactata atgcctcaat ttcttaaggg agatttcatg aacccaggag cgagtttggt
     6901 acgcgcggac ggccgccgga gggagcagat ccgccccgtc aaagtgacca ggaacttcat
     6961 caagcatgcc gagggatcag ttctgatcga gatgggcgat acaaaggtca tctgcacagc
     7021 ttcaattgag gaaaaagtgc ctcctttttt gaaaggaaaa gggacgggat gggtcaccgc
     7081 tgaatactca atgctgccgc gcgccacgca tgaccgctca cctcgcgaag cggtcaaggg
     7141 caaacagggc ggacggacgc tggaaattca acgactcgta gggcgtgcac tgcgcgcggt
     7201 cacggatatg gcgcagctcg gtgagcgttc gatttggatc gactgcgacg tcattcaagc
     7261 cgacggcggc acgcgtacag cctccatcac cggggccttt gtcgcgttag cggacgccat
     7321 ggctatcctc aagacccgcg gtgtgatcaa gaagcgtccg ctcaacgact atctggcggc
     7381 catcagcgtg ggcaaggttg gcggagaaat cctcgtcgat ttggcatata cggaagattg
     7441 catcgcggaa gtggatttga atctggtgat gaccggaaaa ggtcaattcg tcgagattca
     7501 ggggactgca gagaagacgc catttgccaa acaagatatg gatgcatttc tttcacaagg
     7561 ctggggcgcc atacaaaccc tgaccgcatt gcagcgggac ctcatcggcg cgttggagta
     7621 gcgcatggcc cgtgaactgg tcctggctac ccgcaatcga cacaaggggg aggagctggc
     7681 cgcactgttg ggcggattgg acattcgcat ccatacaatg gacgaattcc ctgacgtgcc
     7741 tgatgtcatc gaagacgggg aaacgtgtga ggcgaacgcg atcaagaaag cgcagtcgat
     7801 ttcaaaagcg accggtctct tggctgtcgc ggacgacacg gggttagaag tggatgcctt
     7861 ggatggtcga cccggtgtat atgcggctcg ctatgcagga gaaaacgcga cgtacgaaga
     7921 caactggaaa aagctgttgg gcgagctcac cggtgtcccc cgcgatcggc gaactgcccg
     7981 cttcgtgaca gtcgccgcca tcgtgtctcc gggagagacg gtgcaggtcg tgaccggtca
     8041 cctagacggt ctcatcgccg aggtacccgc cggagctcag ggttttggct atgacccggt
     8101 ctttttcgtg ccacagctag gaagaacgct ggccgaaatg tcgagcgtcg aaaaaaacag
     8161 gatcagccat cgcgcgaaag cctttgcaca agtacgtaac ttgttgcaga atagtctctt
     8221 gcgatcagtc ggggcgtagc gcagcccggt agcgcgcctg ctttgggagc aggatgtcgg
     8281 aggttcaaat cctctcgccc cgaccaatca agctcgctcg ccccggagcc tatttcacat
     8341 tgaggcgacg ggataaaacc tccaattatc tagcccatgc gtgccgcccc cttccgccgt
     8401 cagccatctc gtaattgcgg ctgaacatac ccgcttatag tcataaggtc cggactccag
     8461 gagacattgg cgaggtgctt gcgtcggtta atgtcatcag cgcaggccta cccccccccc
     8521 gtatgatttt tgatacaagg gaaagttttt tcgatagtag tgcgtttcct agggcactcg
     8581 aggtgtgaaa ttgttttact ttagccctga ggagatcggt catgctgcct gacaacaagg
     8641 ttcgggtgct tatcgtcgat gaccatttta ttgttcgaca gacgtttcgc tgtattctca
     8701 agcactaccc caacattgaa gtggtcggtg aggcaagcga tggtgacgag gccattgcgt
     8761 gcgcaggaag attccaaccc tccgtcgtat tgatggatat gaatatgaaa aaaatggacg
     8821 gcatcaccgc ggctcggtta atcaagacac aatatccaca tgtactcgtc ctaggatttt
     8881 cggccgatac gcaaaattac aatgtctatg cgatgcagca agccggggcg tttgaagtgt
     8941 tacagaaaga agatgccatg aaggacttgt atgcggcgat acaaagggga gttgccgccg
     9001 tccaatcgaa cgtcgcgtga tttgcaccct cgcagctctc taacctgcca atcctttttc
     9061 gattcccgct gctctacggc cctccgtttg ccacacatgt cagtgttcca tccgcgtgga
     9121 cgcgtgcaaa tggttgacgg ctgccattat aggtgtcaag aaaccaccta tgtagaagtc
     9181 tctcgtgcca tcctgtactg cacgatggta gagactccac tatccaaggt gggtgtaata
     9241 aacgaaggag cgccgaccga gattggatct caaatgtgca cccgtagctc agttgtatag
     9301 agcatcagtc ttctaagctc accagagggg atagccgaag tcgttgatgg cctgggcaat
     9361 ccctttgtca ttttaaggga ttcgcgcatt gtttcatgcc ttcgattctc gttgtatttc
     9421 tttgtctcgt taaggtcgct ttagcacgtt attctaaaca cccgaataac acctattttc
     9481 actgcccccg gaactaaata atcgtgcctc gcgatacaaa taattt
//
LOCUS       DASTQY010000437         8741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_327350, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000437 DASTQY010000000
VERSION     DASTQY010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8741
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327350"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VFR82_14040"
     CDS             complement(<1..597)
                     /locus_tag="VFR82_14040"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU4502770.1"
                     /translation="MIDGISYLVAAGFGALIGSFLNVCIHRLPRCESIVWPGSHCPSC
                     GTAIEYYDNIPLFSYLWLMGRCRTCRSSISMRYPVVEAANAAGYVIILATFGLTWTTV
                     LYSTLFSALLVVTGTDLTHRIIPNVVTVPGIAIGLLGAATVLPVGLVNAFLGVAIGGG
                     ILWALAWASPYLFGKEGMGGGDIKLLAMIGAFLGWKPAL"
     gene            801..1076
                     /locus_tag="VFR82_14045"
     CDS             801..1076
                     /locus_tag="VFR82_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU4502771.1"
                     /translation="MARIARMKSRTSLADVAPPPRKKRPESLTSREQEILELIWAGFK
                     NKEIGQRLKISVKTVEAHRANMMKKMRVSNTAQLLKTAIQGNIIRVR"
     gene            complement(1060..1905)
                     /gene="rlmB"
                     /locus_tag="VFR82_14050"
     CDS             complement(1060..1905)
                     /gene="rlmB"
                     /locus_tag="VFR82_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU4502772.1"
                     /translation="MKSGNLSRLKASSSRTNPTAPAAGNVDQPELLYGLHAVREALRA
                     GTRPLQRLLVLRTDRQFADLVQQARSQHIPVHIEPPAAFDRLVPDQRHQGIIAFIAAK
                     AYCSTDEILEHARRVDEPPFLVILDGVHDPHNLGAVLRTAEAAGVHGIFIPERRAVGL
                     TPVVAKVSAGALDHMRVARVVNLSRLIEELKGDGVWVYGVEPSAPTDVTTIDMTGPIA
                     FVFGGEGGGIRHGVLQECDGRVRIPMKGKVESLNVSAAVAVTLFEAVRQRRATALIQR
                     PIEPE"
     gene            complement(1826..3328)
                     /gene="cysS"
                     /locus_tag="VFR82_14055"
     CDS             complement(1826..3328)
                     /gene="cysS"
                     /locus_tag="VFR82_14055"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU4502773.1"
                     /translation="MLTLFNTLTMRQEEFRPIDPKHVRMYVCGVTVYDYCHIGHARSA
                     LVFDMLRRYLEYSGYKVTFVKNFTDVDDKIIKRANEQGVSCDLITSKFIDAYYEDMSK
                     LGIKPASKEPRATEHMPEIIAITERLIEKGLAYTLDGDVYFQVDKYPAYGRLSKRRLE
                     ELQAGARVDVDQRKRHPMDFALWKNSKPGEPSWVSPWGAGRPGWHIECSAMSIKHLGE
                     TFDIHGGGMDLIFPHHENEIAQSSGATGKEFAHYWIHNGFVQINQEKMSKSLGNYFTI
                     REIFEKEMQQWPEVVVAETLRYLLLSTHYRGPLDFSNQSLHEAKNALDGFYDLFKRLE
                     EGEQGTAFDEEVRTIIDRAREVYRQKLDDDLNTPMALAVLQNLRTNVNIRFEGWPSET
                     RKRARAAFREFGAVLGLFQLEPGQWQFKPLKVSAVSASDISSVGIDEHVVSVVKMEPL
                     SVKEIEERIAQRLEARKKKDFAKADEIRKSLAAQGIIVEDKPDGTSRWKR"
     gene            complement(3335..3970)
                     /locus_tag="VFR82_14060"
     CDS             complement(3335..3970)
                     /locus_tag="VFR82_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEU4502774.1"
                     /translation="MGIIEAIITILSRFVISTISAWGYTGILVTMAIESACIPLPSEI
                     IMPFSGYLVTTGQFTMLGVTLAGAVGNVVGSILAYYAGVRGGRPFVERYGPYMLLSHR
                     DLETADRWFLKYGEAAVFFSRMLPVVRTFISLPAGIARMNFPRFVIFTFIGSIPWCYA
                     LAYVGVKMGEQWEQLREYFHRFDIVIGLCLALAAGYFLWSHWPRRTETPGS"
     gene            complement(3973..4797)
                     /gene="surE"
                     /locus_tag="VFR82_14065"
     CDS             complement(3973..4797)
                     /gene="surE"
                     /locus_tag="VFR82_14065"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEU4502775.1"
                     /translation="MSEPNSPLILVTNDDGIHSAGLTILAKALGRLGEVWVVAPDRER
                     TAVAHAVTLHKPLRVHEMTKRVYAINGTPVDCVNLALLNILPRRPHLLVSGINKGVNL
                     GDDVLYSGTVSAAVEGTILGVPSMAVSQEGREQFHFVTGAHYARRVARLILQRGLPEE
                     TLINLNVPDRVVGSITGVRVTCLSRRRFDNPIIQKVDPHGKTYYWIAGTRVSWSRSKD
                     ADHEAIEEGAVSLTPLHLDSTHYEVLDRLRSWGPVIQGGQRRRPVSLKSRVRTAKR"
     gene            complement(4901..4977)
                     /locus_tag="VFR82_14070"
     tRNA            complement(4901..4977)
                     /locus_tag="VFR82_14070"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4941..4943),aa:Pro,seq:ggg)
     gene            complement(5119..5466)
                     /locus_tag="VFR82_14075"
     CDS             complement(5119..5466)
                     /locus_tag="VFR82_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU4502776.1"
                     /translation="MGNEPRLGSKVFYKIGEVSQVTKLPAYVLRFWESQFPFLKPKKS
                     RGNQRLYVRRDVETVLEIKRMLYEEGYTLEGVKRYWARRGRVTAQRMKPKELAQKLRG
                     NLQAILKILESHS"
     gene            complement(5509..5784)
                     /locus_tag="VFR82_14080"
     CDS             complement(5509..5784)
                     /locus_tag="VFR82_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEU4502777.1"
                     /translation="MRKADIANEIYKQVGISKNEAADIVELVLNMLKAVLQKGDSVKI
                     AGFGNFVVRSKGARKGRNPRTGEEIGITPRRVVTFRPSQVFKKYVNS"
     gene            complement(5920..7554)
                     /gene="cimA"
                     /locus_tag="VFR82_14085"
     CDS             complement(5920..7554)
                     /gene="cimA"
                     /locus_tag="VFR82_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEU4502778.1"
                     /translation="MAKQVPHSVRKSSVPKKPATKAVSGPLEIYDTTLRDGAQAEDVT
                     FSADDKVMVAQRLDDLGVHFIEGGWPGANPKDIEFFRVIKETPLKQATVVAFGSTRKA
                     SNPVEKDANIQALLGAETKVITLFGKSWSLHVTDALGIALAANLEIISDSIGYLRSKH
                     RRVFYDAEHFFDGYKTNPDYALETIKRAVAAGAERVILCDTNGGTMPWEIKDICRIVQ
                     GECRVPLGIHAHNDCDMAVANSLVALESGVVQVQGTINGIGERCGNANLCSIIPNLEL
                     KMKRPALGDRLHHLKEVSGFVTEIANLMPNKHQPYVGDSAFAHKGGVHIHAVLKNPAT
                     YEHVVPALVGNRQRVLVSDNAGRSGLVEKVEAYGIKLDKNDSKVQELMATLKERENEG
                     YQFEGAEGSFELLMRKAMGAHKPSFQLLGFRVIVEKKQDLGHSGSEATVMVKVGDVTE
                     HAAAAGAGPVNALDHALRKALEKFYPQLREVRLIDYKVRVLSASRGTGSKVRVLIESG
                     DHKDKWGTVGVSENIMEASWQALADSIEYKLLASER"
     gene            complement(7606..>8741)
                     /locus_tag="VFR82_14090"
     CDS             complement(7606..>8741)
                     /locus_tag="VFR82_14090"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU4502779.1"
                     /translation="GNKVIVVLSAMSGETDRLMKLAHDVAALPDERELDMLLSTGERV
                     TIALLAMELRGRGLDARSFTGRQVGIMTDSAHTRARITRVAAERIRDALSRGVIPVVA
                     GFQGINEHSDVTTLGRGGSDLTAVALAGALKADRCVIFTDVDGVYTADPNIVPSARRI
                     NRIAYEEMLEMASLGAKVLQSRSVEFAAKYNVPLEVNSSFKEGKGTLVKREDADMEAV
                     AVSGITGDRNQAKITLVGVPDRPGIAARLLGPVAKANILVDMIIQNVSHESLTDFSFT
                     IPRVDVKKAMPIIEETAKDIGAKSVSVTEAIAKVSLVGVGMRSHSGVAAKMFDVLSHE
                     GVNIMMISTSEIKISCVIDEKYMELAMRALHAAFGLDQSTVGK"
BASE COUNT         1883 a         2572 c         2325 g         1961 t
ORIGIN      
        1 gagcgccggt ttccagccca agaatgcgcc gatcatggcg agtaatttga tgtccccgcc
       61 gcccatcccc tccttgccaa acaggtatgg gctggcccag gccaaggccc agagaattcc
      121 cccgccgatc gccaccccca agaaggcgtt caccaaacca acgggcaaca ccgtggccgc
      181 gccaagcagc ccaatcgcga taccgggaac ggtcacgaca ttcggaatga ttctgtgagt
      241 caggtccgtg cctgttacca cgagcaacgc cgagaacagt gtcgagtaca gcaccgtcgt
      301 ccaggttagg ccgaacgttg cgagaatgat cacatatccg gcagcattcg cggcctccac
      361 gactggataa cgcatggaga tggagcttcg gcatgttcgg catcgcccca tgagccacag
      421 gtagctgaac agcggaatgt tatcgtaata ctcgatggcc gttccgcagg aagggcagtg
      481 agagccgggc cacacgatgg attcgcaccg gggcagtcga tgaatgcaga cgttaagaaa
      541 gctgccgatg agagcgccga atcccgccgc aaccaggtag gaaattccgt caatcatgat
      601 gcacgtcgtt aacttgcacg taccgaccaa tccgtacgtg caacaattga atacgtactt
      661 gtactagggc cattgtgcat tggtttacat gacttagagg actttctata atttccagca
      721 cgcagcgaac gttctccgga agcggcgccc ctggctgcag tatagcgtaa tggatctgcg
      781 atcaaaggag aacatgaaac atggcacgta tcgcaagaat gaaatcccgg acgtctttgg
      841 ccgatgtcgc cccaccgccg cgaaaaaaga gaccggagtc gttaacgagc cgggagcagg
      901 aaattttgga gctcatatgg gccggattca agaataagga aatcggtcaa cggctcaaaa
      961 tcagcgtaaa aaccgtcgaa gcccatcgcg ccaacatgat gaagaaaatg cgcgtatcca
     1021 ataccgcaca gctcctcaag acagccattc agggcaacat cattcgggtt cgataggtcg
     1081 ttggatgagt gccgtagcac gtcgctgccg caccgcttca aaaagcgtca cggcgacggc
     1141 ggcggacacg tttagggatt ccacctttcc cttcatcggg atgcgaacgc gaccatcgca
     1201 ttcctgcaag acgccatgcc ggatgcctcc cccttctccg ccgaatacaa aggcgatcgg
     1261 cccggtcatg tcgatggtgg tcacatcggt cggagcagaa ggttccacgc catacaccca
     1321 gacaccgtcc cctttcagct cttcaatcaa cctggataga ttgacgaccc gggccacccg
     1381 catgtggtcc aacgctcctg ccgacacttt tgccacaacc ggcgtcaacc ccaccgcccg
     1441 acgttcggga atgaagatgc cgtggacgcc agccgcctcg gcggtccgca acaccgcccc
     1501 gagattgtgg ggatcgtgaa ccccgtcgag gatgacaaga aacggcggct catcaacgcg
     1561 gcgggcatgc tcgagaattt catcggtgct gcagtaggcc ttggccgcga tgaaggcgat
     1621 gatgccttga tgcctctggt cggggactaa gcggtcgaaa gcggctggcg gttcgatatg
     1681 aaccggaata tgttggctcc tcgcttgttg gaccagatcg gcaaattggc ggtcggttcg
     1741 cagcacgagg agacgctgga gcggacgagt ccccgctcgc aaggcttcac ggacggcgtg
     1801 gagcccgtac aggagctccg gctgatcaac gtttccagcg gctggtgccg tcgggtttgt
     1861 cctcgacgat gatgccttga gccgcgagag atttcctgat ttcatcagct ttggcgaagt
     1921 cctttttctt tcgggcctca agtcgttggg cgattctttc ttcaatttct ttgacagata
     1981 atggctccat cttgacgacg ctgacaacat gctcatcgat accgaccgac gaaatatctg
     2041 aagccgagac ggcgcttacc ttcaacggct tgaactgcca ttgtccaggt tccaactgaa
     2101 atagtcccaa gacggctccg aattctctaa atgcagcacg ggcccgctta cgagtttctg
     2161 aaggccatcc ttcaaaccgt atatttacgt tcgtccgcaa attctgcaac acagcaagcg
     2221 ccataggggt gttgagatca tcatcaagtt tctggcgata gacctctctg gcgcgatcaa
     2281 taattgttct cacctcctcg tcaaaggctg tcccctgttc gccttcctcc agcctcttga
     2341 acagatcata gaagccatcg agcgcattct tcgcttcgtg caggctttga ttggaaaaat
     2401 ccaacggccc ccggtaatga gtggaaagga gcaaatatcg cagcgtttcc gcgaccacca
     2461 cttcaggcca ttgctgcatc tctttctcaa agatctctcg gatggtaaaa tagttcccca
     2521 gggatttcga cattttctcc tggttgatct gcacaaagcc gttgtgaatc cagtaatggg
     2581 cgaattcctt gccagtcgca ccggaggatt gcgcaatttc attctcatga tgcggaaaaa
     2641 ttaaatccat gccgccacca tggatatcga aggtctcacc cagatgtttg atcgacatcg
     2701 ctgagcactc aatatgccat ccgggacggc ccgcccccca agggctaacc catgagggtt
     2761 cgccaggctt gctgttcttc cagagcgcaa agtccatggg atgccgtttc cgctgatcca
     2821 catccacccg cgcaccggcc tgcagctcct caagtctgcg tttggaaagg cgcccataag
     2881 ctggatattt gtcgacctgg aaatacacat ccccgtccaa tgtataagcc agtccttttt
     2941 cgatcagcct ttccgtgatc gcaatgatct ctggcatgtg ctcagtcgct ctcggttcct
     3001 tcgacgcggg ctttattccc aatttgctca tgtcttcata gtaggcgtcg atgaatttgc
     3061 tcgtaatcag atcacaagag acgccctgct cattcgctcg cttgatgatc ttgtcatcca
     3121 catccgtaaa attcttgacg aaggtgacct tgtagccgct gtattccaag tagcgtctga
     3181 gcatgtcgaa gaccagcgcg ctgcgcgcgt gtccgatgtg gcagtagtcg tacacggtta
     3241 cgccacagac atacatccgt acatgcttcg gatcgatcgg ccggaattcc tcctgcctca
     3301 tcgtcagcgt attaaacagc gtcagcatga catcctacga gccgggggtt tctgtacgtc
     3361 ggggccaatg tgaccagaga aaatacccgg cggccagcgc caggcagaga cctatgacga
     3421 tgtcgaaccg gtgaaagtac tcacgcagtt gttcccattg ttcgcccatt ttcaccccga
     3481 cataggcaag ggcataacac caaggaatcg agccgatgaa cgtgaagata acaaagcggg
     3541 gaaagttcat gcgggcgatg ccggcgggca gcgaaatgaa cgtacgcacc accggcagca
     3601 tacggctgaa gaagacggcc gcttctccgt attttaagaa ccagcggtca gccgtctcca
     3661 aatcacgatg cgaaaggagc atatacggcc cgtagcgttc gacaaacggc cgcccgcccc
     3721 gcacccctgc ataataggca aggatcgacc ccaccacatt tccgacggca ccggcaaggg
     3781 tgacacccag catcgtgaac tggccggttg tgacgagata accggaaaac ggcatgatga
     3841 tctcgctcgg gagtggaata caggcgcttt cgatggccat cgtcaccaga atgccggtgt
     3901 agccccaggc agaaatggta ctgatcacga atcggctcag gatcgtgatg atcgcttcga
     3961 taatccccat gcctaccgct tcgccgttcg tacgcggctc ttcagggaca cgggtcggcg
     4021 tctctgtccc ccctggatga ccggtcccca acttctcaac cgatcgagaa cctcatagtg
     4081 ggtgctatcg aggtggagcg gggtgagcga aacagcacct tcctcgatcg cttcgtggtc
     4141 ggcatctttg cttcgactcc atgagacgcg cgttccggct atccagtagt aggtcttgcc
     4201 gtgagggtcg accttctgga tgataggatt gtcgaatcgc cggcggctca aacacgtcac
     4261 gcggacaccg gtgatcgatc ccacgacacg atccggcaca ttcaggttga tgagtgtttc
     4321 ttccggtaag ccgcgttgca aaatcaagcg cgcgacacgt cgcgcatagt gcgctcccgt
     4381 gacgaaatga aattgctcgc gcccttcttg ggagaccgcc atcgatggca ctccgagaat
     4441 cgtcccttcc acggcggccg ataccgtccc tgaatacaag acgtcgtcac cgaggttgac
     4501 ccccttgttg atgccggaga ccaagaggtg gggccgcctc ggcaggatgt tcaggagggc
     4561 caagttgacg caatcgaccg gcgtcccgtt gatggcatac acgcgtttgg tcatttcgtg
     4621 aacacggagc ggtttgtgca atgtcacggc atgagcgaca gccgtacgtt cccggtcggg
     4681 agcgacgacc cacacctcgc ccagccggcc taaggctttc gcgaggatcg tcagcccggc
     4741 ggaatgtatt ccgtcgtcgt ttgtgaccag aatcaacggt gaattgggct cagacataaa
     4801 aaaagctgcg cggggcagct tcctggcttg agatgattga ccggcgagcc cagtgactca
     4861 gtctactcta ttaacgctca acatccactt caaaaatttt tggtcggggc gagccgattt
     4921 gaacggccga ccactcgcac cccaagcgag tacgctaccg ggctgcgcca cgccccgact
     4981 gaatttttgt ttctacgccg gccacattct cgccttgaag ccggccggat tgcaacctct
     5041 ccctcaaccg gccgtgttgt tgaacaggtg caaacagtgg cggcatgtga acattgtcaa
     5101 ggtgtcatca cggcgtcgtt acgaatgcga ctcgaggatt ttcaggatgg cctgaagatt
     5161 tccccgtaat ttttgggcta attctttcgg cttcatccgc tgggcggtga cacgaccacg
     5221 gcgcgcccag tatcgtttca cgccttccag tgtataccct tcctcgtaga gcatccgttt
     5281 gatttccagt accgtctcga catcacgccg tacatagagt cgctggttcc ctcggctttt
     5341 cttgggcttg agaaatggaa attgtgattc ccagaacctt agcacatagg ccgggagttt
     5401 cgtcacctgg ctgacttcgc cgattttata gaaaactttg ctgccgagcc tgggctcatt
     5461 ccccatggac cggtcctcgt ggcaggagtg gctttaggtg tgatgctgtt acgagttgac
     5521 gtactttttg aatacttgac tgggacggaa ggtcacgact cggcggggcg tgatgccgat
     5581 ttcctcaccg gttcgtgggt tccggccttt cctggcacct ttgcttctga cgacaaagtt
     5641 gccgaagcct gcaatcttga cggaatctcc cttttgtaac acggccttca gcatgttcag
     5701 cacgagttcg acgatatcag cagcttcgtt tttcgaaatg cccacttgtt tgtatatttc
     5761 gtttgcgata tcagcctttc tcatgccccc tcccccttgc tgccaacgtg cactcccgtc
     5821 gacaagagtg tctgccccct cacgcagggg tcagttttac cataagttac gtgaggttag
     5881 ataggctgtc aaggacttat tcttggagag attcagcggc taacgttctg aagccaacag
     5941 tttgtattcg atgctgtctg cgagagcctg ccagctagcc tccataatat tctcggagac
     6001 gccaacggtc ccccacttgt ccttgtgatc gcctgattcg atcagaaccc tgacttttga
     6061 accggttccg cgactggctg aaaggacccg gactttataa tcaatcaatc tgacctctcg
     6121 gagttggggg taaaacttct ccaaggcctt tcggagcgcg tggtccaagg cattgacagg
     6181 gcctgcgcct gccgccgcag cgtgctcagt cacatctccg actttcacca tcacggttgc
     6241 ttctgaacct gagtgaccaa gatcttgttt tttctcaaca attaccctga acccgagcaa
     6301 ctggaaggac ggcttgtgcg ctcccatggc tttgcgcatc agcagttcga aagatccttc
     6361 ggctccctca aactgatacc cttcgttttc ccgctctttg agcgtggcca tgagttcctg
     6421 gaccttcgaa tcattcttat ccagcttgat cccgtaagcc tcgacttttt caaccagtcc
     6481 gctgcgaccc gcgttgtctg acaccaacac gcgttgccga ttgcccacga gagctgggac
     6541 cacgtgttcg tacgtggcag gattcttaag caccgcatgg atatgcacgc cacccttgtg
     6601 ggcaaaggcc gaatcgccga catacggttg gtgcttgtta ggcatcaagt tggcaatttc
     6661 cgtcacgaag ccggatacct ccttcaagtg gtgtaaccga tcgcccaacg caggccgctt
     6721 catcttcaat tcaagattgg gaatgatcga gcacaaattc gcattgccgc atcgttcgcc
     6781 gatgccgttg atcgtgccct gtacttgtac gactccactc tcgagcgcca ccagggaatt
     6841 agccacggcc atgtcgcaat cgttgtgcgc atgaatgccc aacggaaccc ggcactcgcc
     6901 ctggactata cgacaaatgt ccttgatctc ccagggcatg gtgccgccgt tcgtatcgca
     6961 cagaatcacg cgttcggcac cggccgccac cgcacgtttt attgtctcca gggcatagtc
     7021 tggatttgtc ttatagccat cgaagaagtg ctcggcgtcg taaaacactc gtcggtgctt
     7081 tgaccgcaaa tacccgatgg aatcgctgat gatttcgaga ttggcggcga gcgcgatgcc
     7141 tagggcatcg gtcacgtgca acgaccaact ctttccaaac agcgtaatga ctttggtttc
     7201 ggcgccgagg agcgcctgga tgttggcgtc tttctctaca ggattgctgg ccttcctcgt
     7261 ggaaccgaac gccacgacag tcgcctgctt gagcggcgtt tctttgatca cccggaagaa
     7321 ctcaatgtct ttcggattgg cgccgggcca accgccctcg atgaagtgga cgccgaggtc
     7381 gtccaaccgc tgggcgacca tcactttgtc gtcggcggaa aacgtgacgt cttcagcctg
     7441 tgctccgtcg cgcagcgtgg tatcgtagat ttccaatggg ccggagaccg ctttcgtggc
     7501 cggttttttc gggaccgagg attttcgaac ggaatgcggc acttgtttag ccatggcgaa
     7561 gagggtacca ggagcgggcc aggcctgtcg agggtctgcg gggtgttatt tgccgacggt
     7621 gctttgatcc agtccgaacg cggcatggag cgcgcgcatt gccaattcca tgtacttctc
     7681 gtcgatcaca caggagatct taatctcgga cgtgctgatc atcatgatgt tgaccccttc
     7741 atgcgacagc acatcgaaca ttttggcagc cacccccgaa tgcgagcgca tgcccacgcc
     7801 gaccagggaa accttggcaa tcgcttcggt cacggagacc gacttggcgc cgatgtcttt
     7861 tgccgtctcc tcgatgatcg gcatggcctt cttgacgtcg acgcgaggaa tcgtgaacga
     7921 aaaatcggtc agggattcat ggctaacgtt ctgaatgatc atgtcgacca agatattcgc
     7981 cttggcgacc ggacctaaca gccgcgcggc gatacccggc ctatccggca cgccgaccag
     8041 cgtaattttt gcctgattgc ggtcgccggt gatgccggaa acagcgacgg cctccatgtc
     8101 tgcgtcttcg cgtttcacaa gcgtcccctt tccttccttg aaactggagt tgacctcaag
     8161 cggtacgttg tatttggccg caaactcaac tgaacggctt tgcaggactt tcgcgccgag
     8221 actggccatc tctagcattt cttcatacgc gatcctattg atacgtctcg cggagggaac
     8281 aatattggga tccgccgtat agacgccgtc cacatccgta aagatgacgc atcgatcggc
     8341 cttcaacgct ccggcaagcg cgaccgccgt caaatccgac ccgccgcggc ccaacgtcgt
     8401 gacgtcggag tgctcattga tcccttgaaa acctgccacc acgggaatga ctccacggga
     8461 cagggcgtcg cgaatccgct ccgcagcgac cctggtgatc cgcgcccttg tatgggcact
     8521 gtcggtcatg atgccgactt ggcgtcccgt aaacgaacgg gcgtccagcc cgcgcccgcg
     8581 caactccatc gccagcagcg cgatggtcac tcgctcgcct gtggagagca gcatgtccag
     8641 ttcacgttca tccgggagtg ccgccacgtc atgggcgagt ttcatcaacc gatccgtctc
     8701 tccgctcatg gccgagagca cgacgataac cttgttgccg t
//
LOCUS       DASTQY010000438         5908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1715486, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000438 DASTQY010000000
VERSION     DASTQY010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715486"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..780
                     /locus_tag="VFR82_14095"
     CDS             142..780
                     /locus_tag="VFR82_14095"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4502780.1"
                     /translation="MRLSDVIRRWKFRLRRKLQKGSQRRVLDVGSGHRPHEDATHLVD
                     LLPEDNSERGKPIQDLGLPLLVATVESLPFKDKTFDYVYASHVLEHTQDPASACKELM
                     RIARAGYIETPSPFYEQGYNFPHAERGWSFHRWFVYLGEGDTLTFEPKTAKSIDDFCH
                     CRHGQFIRRIYGSLKDLNQLQGVLPRQCSYTVLHWKDEFKFAVKLEGAGTPC"
     gene            774..1796
                     /locus_tag="VFR82_14100"
     CDS             774..1796
                     /locus_tag="VFR82_14100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4502781.1"
                     /translation="MLSVIVAVHNQIGHNRLFLKGIRQYTTGAYEVVVIDNHSTDGSA
                     EFFESEGCRVIRNTANLCYPESMNLGTRNSSGAYVCHINNDLYVGPNWNGRLIEAMER
                     FSLDAITPLGLEMMPTPALTDWIQARWATIGQGRLSSGKSEGQLHAKIRAMYGDWEAF
                     CSQVYLSFSEVIFDGLNGSCVMLRRSTLEKIGLLDERVQAADWDLYYTLRKREEELGD
                     VRRCKIFGGCYVHHFIRATIKGRREPFACTHPRLSIHDKWSREEQERLWYKSNYFQPI
                     SGRRSFRDLLRRHVGKPFEKLAREWQRAVSWRWLYVQPEKIVELHRRQIQVLGTGAWQ
                     RGESSS"
     gene            1793..2578
                     /locus_tag="VFR82_14105"
     CDS             1793..2578
                     /locus_tag="VFR82_14105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4502782.1"
                     /translation="MSAAPSLACILIAKNEAGNIVDCLASVAWADERIVIDAESSDRT
                     AELSRAAGARVIVRPWPGFGAQKNYGIDQVGTEWILILDADERVPPALRDELLERIGH
                     WKAGEPVAFEIPRRNFFYGEWVRHAGVYPDYQIRLFRRGYARYNDVPVHENLIVQGPI
                     GKLASPFDHFTERRIRDHFRKFGHYTTLAAQEKASRVRRVGVSDLLFRPLVVFVKSYV
                     LKRGYLDGIRGLIVSVFAGMYTFVKYAKLWDMTRPSMGVRDAR"
     gene            2578..3717
                     /locus_tag="VFR82_14110"
     CDS             2578..3717
                     /locus_tag="VFR82_14110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4502783.1"
                     /translation="MARPILYIHGVAAVGGAERDLLAILRALDRPDWEPHVACPSEGQ
                     FSNLMGQAGVVLHPMVLPPWRKWFSPFVRWLAVNKLRILLEQLKPALIHVNDIWWVPQ
                     TLEAVRGRPDLRIPVVAHVRQEIEPEKVGRYRLQELDAVIAISRHVERALIAGGVDQR
                     TVRTIYSGIDRVRSGGEMLERTTIYRSVGLPDEAVLLGTVAHLFPRKGYDVMLKALPS
                     IIRTVPAVHYVVIGTGDGGYEQRLRELSAELGISDHVHFVGFQQDVNPFLEAMSLYVH
                     PARMEGFGIAVVEALAAGKAVVATNVGGLPEVVEEGDTGLLVAPDDSQALTAAVLSLL
                     RDDGRRRAMGERAVKWVRERFDLRASVRAMERFYQDVLSAHRNRT"
     gene            3714..4817
                     /locus_tag="VFR82_14115"
     CDS             3714..4817
                     /locus_tag="VFR82_14115"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU4502784.1"
                     /translation="MRIGIDASPVAGDRGGVGWHTYYLLRAMLAAPSEARFVGYMRPG
                     TSPPSEASQWTGVERLEWVTASKWAMTGRGTADRLDLYHGTNFRMHTVGRFGGIVTIH
                     DLWLERFPEYSSKLLGQRLSSYKTKRIARRAKKVVTVSQFSARELISVYGLSEQHIVV
                     IPNGVSEEFSPRHNAAEMSVLKQRMGLKTDRFILFVGGADPRKNHRKVLEAASLIRTH
                     LDGRTVLLVGSPTHPFGSYERTAASYGLTAHVLCPGRLSREDLRLLYSYADLFVFPSL
                     YEGFGMPVLEAMACGAPVITSNDTALGEVAGDAAVVIDPHDARALANSMVQVLENDSY
                     RISLREKGFLRVQDYRWSHVAARTLSLYSSLCR"
     gene            4828..5847
                     /gene="rfaQ"
                     /locus_tag="VFR82_14120"
     CDS             4828..5847
                     /gene="rfaQ"
                     /locus_tag="VFR82_14120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HEU4502785.1"
                     /translation="MAPHNILTIKLRYLGDVLLATPTWHALKAAYPGARLTVVVNRGT
                     EDILLSNPHVDEVLSLERGSLMQQYRFIANIRRRRFDTVVDLTDGDRAALLTWISGAR
                     VRIGFNAEHRWTGRCYTRVITSDAGAHRIERDLSALAPLAIPVIERIPRMWLAEEDDA
                     AADDLLRRLGVRSDRPWVVIQPGARYWFKAWPSERFAELADRVVDRYGCQVLVAGSPE
                     EAALAQTVVIQAKQRLISIAGLSRVRTLAALLKRSALFVGNDTGAMHIAAAVGTPVVG
                     LFGPSNPMEWGPRGGPTEVIYKGLDCRECFHPTCRRGEQNCMKLISVSEVMAAAERLL
                     THGLR"
BASE COUNT         1221 a         1563 c         1818 g         1306 t
ORIGIN      
        1 cgtctcccac agcggtttcc caagtcgagg gatgtctttc gcaagtatgg acaatactct
       61 ctctttgtcc attaacctcg gcgcattcct caagctgaat aacgtctcgt acgagatgcg
      121 actctgaaac ggagcaaggt aatgcgattg tccgatgtta tacgtcgttg gaagtttcgc
      181 ctcaggcgga agctgcaaaa aggatctcaa cggcgcgtct tggatgtcgg atccggtcat
      241 cgcccgcacg aggatgctac ccacttggtt gacctccttc ccgaggacaa ttcagagcgc
      301 gggaaaccga tacaagattt gggccttccc ctcctggtgg ccacggttga atccctgccg
      361 ttcaaagaca agacgtttga ctacgtctat gcctcccatg tgttggagca tacccaggac
      421 cctgcctccg cgtgcaaaga actcatgcgc attgcacggg ccggctacat cgaaacgcca
      481 agtccgttct atgagcaggg atacaatttt cctcatgcgg agcgcggttg gtcgttccac
      541 cggtggttcg tctatttggg cgaaggcgac acgctcacat tcgaacccaa gacggccaag
      601 tcgattgacg atttttgcca ttgccgtcat ggacaattca tcaggcgaat atacggttcc
      661 ctgaaggatc tcaaccagct gcagggtgtg cttccccggc aatgtagcta tacggtgctg
      721 cattggaagg atgagttcaa attcgccgtc aagctggagg gggcaggtac gccatgttga
      781 gcgtaatcgt cgccgtccac aatcagatcg ggcacaatcg actgtttctc aaaggcattc
      841 gccaatacac gaccggtgcc tatgaagtgg tggtcatcga caatcattcc accgacggct
      901 cggctgagtt ttttgaatcg gaaggatgcc gggtcatacg aaacactgcg aatctgtgct
      961 atcccgaatc catgaatctc gggacacgaa attcgagcgg ggcctacgtg tgccatatca
     1021 acaacgatct gtatgtcgga ccgaattgga acggacggct gatcgaggca atggagcgct
     1081 tcagtctgga cgccatcacg cccttaggct tggaaatgat gccgacgccg gcgctgacgg
     1141 attggataca ggcacgctgg gcaacgatcg ggcaaggccg tctctcatcc ggaaagagcg
     1201 agggccaact ccacgcgaag attcgtgcga tgtacggcga ctgggaagcg ttctgcagcc
     1261 aggtctatct gagtttctca gaggtcatct tcgatgggct caacggctcc tgcgttatgc
     1321 ttcggcgttc caccctcgag aaaatcggat tgttggacga acgagttcag gcggcggatt
     1381 gggacttgta ttacaccctt cgaaaacgtg aagaggagct gggggatgtc cgacgctgca
     1441 aaattttcgg aggctgctac gtccatcact tcattcgagc aaccatcaaa ggaagacgag
     1501 agccttttgc ctgtacgcac ccgcggctgt cgatccatga taaatggtcg agagaagagc
     1561 aggaacgatt gtggtacaag tccaattatt ttcagccgat ttctggccgg cgttcgtttc
     1621 gggatctcct gcgtcgacac gtcggcaagc cattcgagaa attggcgagg gagtggcagc
     1681 gggccgtgtc ctggcgctgg ctgtatgtgc agcctgaaaa gatcgtcgag ttgcaccgcc
     1741 gccaaattca ggttctcggc accggggcct ggcagagagg cgagtcatcc tcgtgagcgc
     1801 agccccgtcc ttggcctgta ttctgatcgc gaagaacgaa gccgggaata tagtagactg
     1861 cctcgcctcg gtcgcctggg ccgacgagcg gatcgttata gatgccgaaa gcagtgaccg
     1921 cacggccgaa ctttcgcgtg ccgccggcgc aagggtgatc gtacgaccat ggccgggttt
     1981 tggcgcgcaa aaaaactacg gcatagatca ggtcggtacc gaatggattc tgatcctgga
     2041 tgcggatgag cgggtgccgc cggctttgcg cgatgaactc ttggagcgga tcggccactg
     2101 gaaagcaggg gaacccgtgg cgtttgaaat cccgcgcagg aatttctttt acggagaatg
     2161 ggtgcgccat gccggtgtct atcccgatta ccagatccgc ctcttccgac gaggttacgc
     2221 caggtataac gacgtccccg tgcatgagaa tctgatcgtc caaggaccga ttggaaaact
     2281 ggcgagtccg tttgaccact tcaccgagcg ccgcatccgg gaccattttc gaaagtttgg
     2341 ccattacacg acactggcgg cccaggagaa agccagcaga gttcgccgtg tgggagtctc
     2401 ggacctgctc ttccggccgc ttgtcgtctt cgtaaaaagt tatgtgctca agcggggcta
     2461 tcttgacggc atacgcggtc taatcgtgtc cgtctttgca ggcatgtaca cgttcgtcaa
     2521 gtatgccaag ttgtgggaca tgacccgacc ttcaatgggc gttcgggatg cccgctagtg
     2581 gcccgaccaa tcctgtatat ccatggggtc gctgccgtgg gcggggcgga acgtgatctg
     2641 ctggccatac ttcgggcact cgacagaccc gactgggaac cgcatgtcgc ttgtccctct
     2701 gagggacagt tctcaaatct catgggtcaa gccggcgtcg tgcttcatcc gatggttctc
     2761 ccgccttggc gcaaatggtt ttcgccgttc gtccggtggt tggcagtcaa caagcttcgg
     2821 atcttgcttg agcagctcaa gcctgcgctg atccacgtga atgatatctg gtgggtgcct
     2881 cagaccctcg aagcggtccg cggccgtccc gatcttcgaa taccggtcgt ggcgcatgtc
     2941 cgtcaagaaa tcgagccgga aaaggtcgga cgatatcgcc tccaggaact ggacgccgtg
     3001 atcgcgattt cgcggcacgt cgaacgggcg ttgatcgccg gcggcgtcga ccagcggacc
     3061 gtccggacga tttatagcgg aatagatcgg gtcagaagcg gtggtgaaat gctcgagcga
     3121 acaacaatct accgctcagt cggtttgccc gacgaagccg tcttgttggg aacggtagcg
     3181 catctgtttc cgcgcaaggg gtatgacgtc atgcttaagg cactgccgag tatcatacgg
     3241 actgtaccgg cggtgcacta tgtggtcatt ggcacaggcg acgggggcta tgaacagagg
     3301 ttgcgagagt tgtcggccga actcgggatt tcagaccacg tccatttcgt cggatttcag
     3361 caggatgtga atccgttcct ggaagcgatg tccctatatg tccatccggc gcgcatggaa
     3421 ggttttggca ttgcagtcgt ggaagctctc gctgcgggaa aagccgttgt cgcgacgaat
     3481 gtgggtgggc ttcccgaggt tgtcgaggag ggagacaccg gactcttggt cgcgccggat
     3541 gattcccaag cattgactgc tgcggtgctg tctctactgc gtgatgacgg tcggagaagg
     3601 gccatgggcg agcgagccgt gaaatgggtc cgcgagcgat tcgatctgcg agcgtccgtg
     3661 agagcgatgg aacggttcta tcaggatgtg ctctcagcgc accggaaccg aacatgagga
     3721 tcggcatcga tgccagtcca gttgcgggcg ataggggagg ggtgggttgg catacctact
     3781 atttgctgcg agccatgttg gccgctcctt cggaggctcg cttcgtggga tacatgagac
     3841 cgggcaccag tccaccatcc gaggcctccc agtggacggg cgtagaacgt cttgagtggg
     3901 tgacggcttc gaagtgggcc atgaccgggc gaggaacagc cgatcggttg gatctgtacc
     3961 atgggacgaa ttttcgcatg catacggtgg ggcggtttgg cggcatcgtt acgattcatg
     4021 atctgtggct cgaacgattt cctgagtatt cgtccaaatt gctcggccag agattgtcgt
     4081 catacaaaac gaagcgaatc gcacggcgcg caaaaaaagt tgtaaccgtt tcccagtttt
     4141 cggcgcggga attgatatct gtgtacggtc tgtctgaaca acacattgta gtcatcccga
     4201 atggtgtctc cgaagaattt agcccgcgac ataacgcggc ggagatgagc gtcttgaaac
     4261 agcgcatggg gttgaaaact gatcggttta tcctatttgt cggaggcgca gatccacgaa
     4321 agaaccatcg aaaggtcctc gaggccgctt cactcattcg aacacatctg gatggtcgaa
     4381 cggttctgct tgtcggttca ccgacgcatc cattcgggtc gtatgaacgg acggctgctt
     4441 catacggatt gactgctcac gtcctgtgtc ctgggcgatt atcgagagag gatttgcggt
     4501 tgctctactc ctatgccgat ctctttgtgt ttccatccct gtatgagggc ttcggtatgc
     4561 cggtgttaga ggccatggcg tgcggtgcgc ccgtcatcac atccaacgac actgctctgg
     4621 gcgaggtggc cggcgacgca gcggtggtca tcgatccgca tgatgcacgg gcattagcca
     4681 actccatggt ccaggtgttg gagaacgaca gctaccgcat ctcactgaga gagaagggtt
     4741 ttcttcgagt acaggactac cgctggtccc atgtggccgc gcggaccctg tcgttatact
     4801 cgtcgctctg ccggtagaat tggccgcatg gcaccgcaca acattctaac catcaaattg
     4861 cgctatctgg gtgatgtgct gctggcgacg ccgacatggc acgccctgaa agcagcttat
     4921 ccgggagcca ggttgaccgt ggtcgtgaat cgggggacgg aggatattct actgtccaat
     4981 cctcatgtcg atgaggtcct ttcactggag cgcggttcac ttatgcagca gtaccgtttc
     5041 attgcgaata tccgacgacg tcgcttcgac acggtcgtgg atttgaccga cggcgaccgg
     5101 gcggcattgc tcacgtggat cagcggcgca cgcgtgcgca tcggctttaa tgcggagcac
     5161 cggtggaccg gacgctgtta cacgcgtgtc atcacaagcg acgcaggcgc tcatcgaatc
     5221 gaacgcgatt tgtcggccct ggccccgctg gctattcccg tcatcgaacg catcccgaga
     5281 atgtggttgg cagaggaaga tgacgcagcg gcagacgatc tccttcgacg tctcggtgtg
     5341 cgatcggaca ggccgtgggt agtcatccaa ccgggcgctc gttattggtt taaagcctgg
     5401 ccgtcagagc gctttgccga gctcgccgac cgagtggtcg accggtatgg atgtcaggtg
     5461 ttggtggctg gcagcccaga agaggcggcg ttggcgcaga cggtcgtgat acaggcgaag
     5521 cagcggttga tctccatcgc cggtctgtca cgtgtcagga cgctggcagc gctgctgaaa
     5581 cggtcggcgt tgttcgtcgg taacgacacg ggggccatgc atattgccgc agcagtggga
     5641 acgccagtgg tgggactgtt cgggccctcg aatcctatgg aatggggacc acggggcggc
     5701 cccacggaag tgatttacaa aggtcttgat tgccgagagt gttttcatcc aacctgccga
     5761 cggggggagc aaaactgcat gaagctcatc agtgtgagcg aagtgatggc ggctgcagag
     5821 cgattgctga cacacggact acgctgaaag gcattaggct cgcgggaggg tattggtggg
     5881 cgcgattctt gacatcggtt gtggagcg
//
LOCUS       DASTQY010000439         5880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_805472, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000439 DASTQY010000000
VERSION     DASTQY010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805472"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..622
                     /locus_tag="VFR82_14125"
     CDS             245..622
                     /locus_tag="VFR82_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502786.1"
                     /translation="MWKRNFMFRAADATPLNESENELFHETDPAMDSAGLQLEKFLSV
                     WIQGDGDDEHPVAFTNMYVRTATLDFEKRVGFLQPLQGRTHQIKQLLTPAQKSYLRTW
                     LSSAAPRAWEAADEHFKMLLDLE"
     gene            619..1182
                     /locus_tag="VFR82_14130"
     CDS             619..1182
                     /locus_tag="VFR82_14130"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU4502787.1"
                     /translation="MIPPDVHVFVPCRSVHYALASVLCLALALPGGVEGAGVEVLYAP
                     DDAPLDRVVTLYQQAKHYIYVSVYGLTYPRAVEALVAAKKRGVDVRMLTDQERTQEFK
                     QHSALQTLKLAGIPVRVNQHEGLMHLKQVVIDDEINTSGSMNHTTSGNLYNDERMDII
                     TDRILSQKARDHFLSMWNDRARFIDWK"
     gene            1213..2475
                     /locus_tag="VFR82_14135"
     CDS             1213..2475
                     /locus_tag="VFR82_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU4502788.1"
                     /translation="MIEVTDVAIVGAGGGGAVLALALAQKGIHTIVLDQASGPPRGLR
                     GEILQPNGQQVLDELGVLRSLPADAVRSVKTFHFCRAGGTRLCSIDYGALPPPYNQAI
                     VTLPNVAHQAIVDALHTQATVSLRYDTSFVGLRTERNRVVGLTVQGPQGPYPIKAKLV
                     VGADGAFSRVREALKIPAELHLYPEGYLIAILESQEPVSEAFYYVGHKEILGLFPATG
                     NMVYLFYMIPSGSYEALKGSSTSSLHARWTAIAPQFRALFERLSDWKQTAYMPTGRVR
                     AATWVADGAVLIGDAAHAMNPHASQGRMQAMVDAMTLAELLPSCLADGDCSAGRLQVY
                     ETARRPQVTMLQRLADQQVFYWNTGNPLIAYLRDRVFRTLDRNSRLRYQVLSTTAGLR
                     QTEPFSIMDRVIATGFLPDLVNMIPRTS"
     gene            2486..3229
                     /locus_tag="VFR82_14140"
     CDS             2486..3229
                     /locus_tag="VFR82_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502789.1"
                     /translation="MGTTERIIEGLPEETQKLLQAYAKDVKAALNDQLEGLLIYGSAV
                     RGEFLTGRSNLNMVLVVNAYDAALLKRYAPVHKRWSKEGIITPLFLTDHDAKLFSALF
                     PLEFLEIQEHHRVLGGRDPFVGFHVDTRRLQDAVMQGLAGHVFRLRQRYAEAGGADDA
                     VTILLPLAITSTIPLLRGLQRLQGWPVLTQSDAVIKDIADRLQLDLQGFHEALLLKRG
                     QITPGPSEIPRVFDRYLQAATMLAEVVHR"
     gene            3313..3954
                     /locus_tag="VFR82_14145"
     CDS             3313..3954
                     /locus_tag="VFR82_14145"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU4502790.1"
                     /translation="MKLPDPMGYVSDHAKVLEDDWRARIRSVAQDLERKTGVEMVVVT
                     VSTVKPFRSAQDYATAIYEKWGIGSAQQEHGILVLLAVEERQAALTLGRQMLPVITPA
                     VMNQIGTEYLHPAIDRGHFGDGLYRTVVALASHSQDIRAGTPSRTHYRGMGFWITLFT
                     SIGAIAFFWWISRPDLRHPYRRIQRGEYWGTGQGGFGGNWGGFGGGTNGEGFK"
     gene            4249..5862
                     /locus_tag="VFR82_14150"
     CDS             4249..5862
                     /locus_tag="VFR82_14150"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HEU4502791.1"
                     /translation="MGAPLYSHTWVALVVWLCSSAFLLNTVPAGAAETLTLTAPKEPA
                     RPEDTFVRMFPGLLPFAPATDEMREKARKLGEKGGVIDAKDDMLNTTPLNSILNPVSS
                     NPDNHTMTAGVTFLGQFIDHDLTLDPRSPLGEPADPRRTRNFRTAAFDLDSVYGNGPL
                     GSSQFYDQSSGDIKFKVEAIPGSEAVSRKGAVRYDVPRDVNNTAIIGDSRNDENVIIS
                     QLHLAMLRFHNAVVDHLRTKQGLGNLSAEQIFKMAQRLVRWHYQWIVIHEFLPLTIGQ
                     ERVNEILTRGPKFYNPHDRQLQNAEGNPLIPIEFSVAAYRFGHSQVRPSYRLNFGPQP
                     QGDVFVFAFLDSEDPNKNDTDDLRGGKRAARRFVDWQTFFNFGDNLFRPNKKIDSKLS
                     TVLLELLGSRGPAPGMPSDGELSLASRNLMRHVNFGLPSGQAVARVMGAPVLTQAQLA
                     ELQPFGMDRSTPLWFYILKEAELMEAGLRLGPVGARIVGEVFIGLLQADSDSYLVAER
                     NWKPVLPSATPGDFRVTDLLKFAGVVPPL"
BASE COUNT         1257 a         1666 c         1653 g         1304 t
ORIGIN      
        1 ccaccatctg cggctcgatc tttttggtct tcagcaaatc gaggacactg cctccgggaa
       61 actcctcaga cttgccgttg atttttacct gcatggatct cctaacttgg cccaggaatt
      121 aaggaattcg gacagagtaa caaagtggct ttcggcttgt caaggcgacg accgctcgcg
      181 agcgttggct tgactttgcg cttggaccgc cgccactatg ccggttccct ggagggatgt
      241 tggcatgtgg aaacgcaact ttatgtttcg cgccgcggat gcgacgccgc tgaacgagtc
      301 cgaaaacgag ctgtttcacg aaaccgatcc agccatggac agcgccggcc tacagttaga
      361 gaagtttttg tccgtttgga ttcaagggga cggcgatgac gagcacccgg tcgcctttac
      421 gaacatgtat gtacggactg ccaccctgga tttcgagaag cgcgtcggat tcctccagcc
      481 tttacagggg cgtacgcatc aaatcaaaca actgctcacg ccggctcaaa aatcttatct
      541 gcgcacatgg ctttcgtctg ctgctcctcg agcctgggaa gccgcggacg agcattttaa
      601 gatgctgctt gacttggaat gatcccgccc gacgtccatg tcttcgtccc gtgtcggagc
      661 gtccactacg ccctcgcatc cgtgctttgc cttgcgctgg cacttcccgg tggcgtcgag
      721 ggtgccggcg tcgaagtgct gtatgcgccg gacgatgccc ccctggatcg tgtcgtcaca
      781 ctgtatcagc aggccaaaca ctatatctat gtttccgtgt atggattgac gtatccgcgc
      841 gccgtggaag ccttggttgc agccaagaag cgcggcgtgg atgtacgaat gttaaccgat
      901 caggagcgca cacaggaatt taagcaacac agcgcgctgc agaccttgaa attggctggc
      961 attccggtcc gtgtcaatca gcacgaaggc ctcatgcatc ttaaacaggt ggtcatcgac
     1021 gacgaaatca acacatcggg ctccatgaac cacacgacca gcggtaacct gtacaatgac
     1081 gagaggatgg acatcatcac cgatcgcatc ctctcccaaa aagccagaga ccacttcttg
     1141 tccatgtgga acgatcgcgc cagatttatt gactggaaat agagaggaac atgcagggtc
     1201 aggtggcaag cgatgattga agtgacggac gtggcgatcg tcggagcggg aggcggcgga
     1261 gccgtgttgg cgctcgctct ggctcaaaaa ggcatccaca caattgtcct ggaccaggcg
     1321 tccgggccgc cacgcggatt gcgcggggag atcctgcagc cgaatggaca gcaggtactc
     1381 gatgaacttg gggttctacg atcactgccg gcagatgcgg tgcggtcggt taaaacattt
     1441 cacttctgtc gtgccggcgg cacaagattg tgttcgatcg attatggtgc ccttcctcct
     1501 ccctataatc aggccattgt gacgttaccg aacgttgcgc accaagcaat cgttgatgcg
     1561 ttgcacacac aggccacggt cagcctccgc tatgacacgt cgttcgtcgg gctgcggaca
     1621 gagcgcaacc gagtggttgg actcaccgtt caaggaccac agggacctta cccgatcaaa
     1681 gccaaactcg tcgtgggggc cgacggggcg ttttccaggg tccgcgaggc gctcaagatt
     1741 ccggctgagt tgcatttgta tccggaaggc tatttgatcg ccattctcga gtcacaggag
     1801 ccggtatcag aggcgtttta ttatgtcggt cataaagaaa tcctcggctt gtttcccgcg
     1861 accggcaaca tggtctacct gttctatatg atccccagcg ggtcgtatga ggctctcaaa
     1921 ggcagcagta cgagttcctt gcacgctcgc tggaccgcca tcgcaccgca gtttcgggcg
     1981 ttatttgaac gcctgtccga ctggaagcag accgcctaca tgccgacggg cagagtccgt
     2041 gctgccactt gggttgctga tggagcggtg ttgatcggcg atgcggcaca tgccatgaat
     2101 ccgcacgcct ctcagggaag aatgcaggcc atggtcgatg cgatgacgct tgccgagctc
     2161 ttgccctcgt gcctcgccga tggggactgt tccgcgggcc gcttacaggt ctatgaaaca
     2221 gcgcggaggc cacaggtcac gatgttacag cgtctcgctg atcagcaggt cttctactgg
     2281 aacaccggaa acccgttgat cgcctattta cgggatcgag tatttcgtac gttggatcgc
     2341 aattcgcgac tgcggtacca agtactttca acaacggccg ggttacgcca gactgagccg
     2401 ttttcaatta tggaccgggt tatcgcgaca ggatttttgc cggatctggt caacatgatt
     2461 ccgcgtacat catagccgag gacgcgtggg cactaccgag agaatcatcg aaggcttgcc
     2521 agaagagact caaaagctct tacaggccta tgcaaaggat gtcaaagcgg cgctgaacga
     2581 tcagctcgaa gggctgttga tctatggcag tgccgtccgg ggagaatttc tcaccggtcg
     2641 ttcgaatctc aatatggtgt tggtcgtcaa cgcctatgat gccgcgctct tgaaacgcta
     2701 cgcaccggtc cacaagcgtt ggagcaagga gggcatcatc acgccgctct ttctcaccga
     2761 ccacgacgcc aagttgtttt ctgcgctctt tccacttgag tttctcgaga tacaggagca
     2821 ccacagagtg ttgggcggcc gtgatccctt tgtggggttc catgttgata cgaggcggtt
     2881 gcaggacgct gtcatgcagg gacttgccgg ccatgtgttt cgattacggc aacgatatgc
     2941 tgaggccggc ggagcggacg acgccgtgac gatcttgttg ccacttgcca ttacctcgac
     3001 catcccgctg ttacggggat tgcaacggct acaaggatgg cccgtgctca cccaatccga
     3061 cgcagtgatc aaggacatcg cggaccggtt acaactcgat ttgcagggat ttcatgaagc
     3121 cttgctgttg aaacgaggac agatcactcc cggcccgtca gaaatacctc gagtgtttga
     3181 ccgctatttg caggctgcaa ccatgcttgc cgaggttgtc catcgatgaa ccgccaccta
     3241 ccgatttgga tcgttgtcat gctccatctg gcggaggcat gggtttgggc gagtccttac
     3301 gagcgtccaa ccatgaagct gccggacccc atggggtatg tcagcgacca tgccaaggtt
     3361 ctcgaagacg actggcgcgc aaggatccgt tcggtagccc aagacctcga acgaaaaacc
     3421 ggcgtggaaa tggttgtggt gacggtgtcc acggtcaagc cgtttcgctc cgcacaggac
     3481 tacgccaccg ccatctatga gaagtggggc attggatctg ctcagcaaga gcacgggatt
     3541 ttagtcctgc ttgcggtgga agagcggcag gccgcactga cgctcggcag acagatgcta
     3601 cctgtgatca cgccggcagt catgaatcag attggcacgg agtatttaca tcctgcgatt
     3661 gatcgaggcc acttcggcga cgggctgtat cgaacggtcg tcgccttggc atctcacagt
     3721 caagacatcc gtgccggcac gccctcgagg actcactata gaggcatggg attctggatt
     3781 accttgttca ccagcatcgg cgctattgca tttttctggt ggatcagccg gcccgattta
     3841 cgtcacccct accgtcgcat tcaacgtggt gagtattggg gcacggggca gggaggtttc
     3901 ggcgggaact ggggcgggtt cggaggtgga acgaatggag aagggttcaa atagcttccg
     3961 tttccaatgc attcagcaat gattcccccg ttttgtcgca ttcgagcgac tgcactcatc
     4021 gcctaccaac taatcagatg ttctcttccc cagctccgaa gcaaatctaa aagcgctcgc
     4081 attgacaagg cgctcctctc actctgttct cgtaggactg cagtttttac aggcaccccg
     4141 cggaggcgct agtcgtaagt acggattgaa aatacacccc atgctggtat atctgttgct
     4201 cctcgcgcag gggaccgctg ggcaatacct ttggaaacag gagggtatat gggggcacct
     4261 ctttactcgc acacctgggt cgctctggtc gtatggctgt gctcgtcggc gttcctgctc
     4321 aatacggtgc ctgccggagc cgccgagacc ctcacactga ccgctccaaa ggaaccagcc
     4381 agaccggagg ataccttcgt caggatgttt ccaggccttc tgccgttcgc gcccgcaacc
     4441 gatgagatgc gggagaaagc ccggaaactg ggcgaaaaag ggggtgtcat cgatgccaag
     4501 gatgacatgc tcaacacaac tccacttaat tccattctca atcctgtttc cagcaatccg
     4561 gataatcaca ccatgacagc cggtgtcacg ttcctcggcc agttcatcga tcacgatctc
     4621 acgctcgatc cgagatcgcc cctcggtgaa cctgccgatc ccagacgcac gaggaacttt
     4681 cggacggcgg cgttcgatct ggacagtgtc tacggaaatg gtcctctagg atcgtcgcag
     4741 ttttacgatc aaagctccgg cgacattaag ttcaaggtag aggccattcc cggttcggaa
     4801 gctgtgtccc gcaaaggagc ggtccgctac gatgttcccc gcgacgtcaa caataccgcc
     4861 attatcggcg acagccggaa cgatgagaac gtcatcatca gtcaactgca tctggccatg
     4921 ctgcggtttc acaacgcggt cgtcgatcac ctgcgcacca aacaaggcct cggtaatctc
     4981 tctgccgagc agatcttcaa gatggcgcaa cgccttgtcc gctggcatta ccaatggatt
     5041 gtcattcatg aatttcttcc gttgaccatc ggccaggaaa gagtgaacga aatcctgacg
     5101 cgcggaccga agttctacaa tccccatgat cgacaattgc aaaacgcaga aggcaatcca
     5161 ctgattccga tcgagttttc ggtggcggct taccgctttg ggcattcgca ggtccggccg
     5221 agctatcggt tgaacttcgg accccaaccc cagggcgatg tctttgtctt tgcctttcta
     5281 gactcggaag atcccaacaa gaacgacacg gacgaccttc gaggcggaaa gcgtgcggcg
     5341 cgccgcttcg tcgattggca gacgttcttc aatttcggag acaacctttt ccggccaaac
     5401 aaaaaaatcg acagtaaact gtccaccgtt ctccttgagc tgctcggctc acgaggtcca
     5461 gcaccaggaa tgccgtccga cggcgagctc tcactggcgt cgcgcaattt gatgcggcac
     5521 gtcaacttcg gtctcccttc ggggcaggcc gtcgctcgcg tgatgggggc accggtactg
     5581 acacaagcac aattggccga gctgcaaccc ttcggtatgg atcgcagtac gccgctgtgg
     5641 ttctatattc tgaaggaagc agaactgatg gaggccggcc ttcgcttggg acccgtgggc
     5701 gctcgaattg tgggtgaggt gtttatcgga ttgttacagg cggacagcga ctcctatctg
     5761 gtcgctgaac ggaactggaa gcccgtcctt ccatccgcga ccccgggcga cttccgcgtc
     5821 actgatttgc tgaaattcgc cggggtggta ccgccacttt aaccgtcgct gtctccgtcc
//
LOCUS       DASTQY010000440        10433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_591241, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000440 DASTQY010000000
VERSION     DASTQY010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591241"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VFR82_14155"
     CDS             complement(<1..447)
                     /locus_tag="VFR82_14155"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEU4502792.1"
                     /translation="MPILFRYLLREYAKIFLMCFAGLMTIYLVIDFFEKVRRFLSFDA
                     TWLDVLSYFALKIPGVSYQIVPLAVLMATLLTLGLLSRNNEITAIRSCGISLPWIASP
                     FLTFAVLMSLVLLLFSSTVIPLATNKAEEIRVIRIEKKPPSAVLTLS"
     gene            complement(454..1542)
                     /gene="lptF"
                     /locus_tag="VFR82_14160"
     CDS             complement(454..1542)
                     /gene="lptF"
                     /locus_tag="VFR82_14160"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEU4502793.1"
                     /translation="MTLRTLLDRYIFTELLYPFSVSLGMLCFIMLTRELLRLVELLVT
                     KGVGLWAVLNVFAHLLPSFLVLTLPIAGIIASITAFGRLSLDKELVAMRAAGLSLLRL
                     ARPVMLFALLVCGMTLWLAQWGQPWSSINLKKVALSLLRDQLVLALERGSFTEPIPGM
                     TIYVSDKGEQPSGGIFISDGRIASEPRVIVAQDYRVLIDSASDQVALRLQDGVIHTKP
                     DEIDEYQLASFSSYDLKLSLNQSGYASTEERPSYDQLVARLNASQWRDPWALRRLTEY
                     YKDMAFPTASLILCMLGLPVGIVSKRSGRIGGFAVGVLVIIAYYVLNVACEFLVTATA
                     IPPFAGAWLPNSVFLVITLVSFYRMSKQ"
     gene            1850..2068
                     /locus_tag="VFR82_14165"
     CDS             1850..2068
                     /locus_tag="VFR82_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEU4502794.1"
                     /translation="MKTKGTVKWFNDRKGFGFIRLDSGEDVFVHYSALQGEGFKSLKE
                     GENVEFDIVQGAKGPQAANVMKMSIAAS"
     gene            2224..4389
                     /locus_tag="VFR82_14170"
     CDS             2224..4389
                     /locus_tag="VFR82_14170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4502795.1"
                     /translation="MGIDRSKVLHSAQLFASKGQFDAAISEWKKLSAESPGDGSIHNS
                     IGELHLKRNSTADAVASFIQAANAFRAEGATLKAIATFKKVLKLDPSRYDMYRFLGDL
                     NAERGLLSSAVQDYLTLGKHYLKERKTKEALDIYKKIVSQDPSNLDAQQRVAELCVQE
                     NMQDEATRVYLQLGRERSGQQRYAEAKEAYESVLRIDPSNSEAQQFIEHYKKTGGTPP
                     PRTTKPGSAPAAHTGEPTDLLAEASRRIEEKQYAGAEAILNQMLTKEPGNPQVCQLLA
                     RLHLQQGQLQVALGEYRFLAGAALRAQDYPQAELLIREFLTVEPNSVPLLELQGELYE
                     EKGEIESAIQHYGKAIEVLLKTPEPGMPTLHEEIFEKVQSLAPDSPMAKRLAALMRGD
                     ATVEPAAAPAAATIGGFSIEGATPDDAWNMTSAIKPTQDRHLTGQGAPASLPPSSPST
                     PEVFSVENATPDDMPSLSHKAPEPVASSQPAPSISEQFQAYSESGQWAQAEQWLGTVV
                     SAQPEDPHPRELLGQVLERKGDGAGAALQYARALELMSVGQDSVVNGPIRHLYDKVKE
                     LAPASPIVARLSALLAPAAPVDDVDADTHYTLGVAYKNMGLIEEAKEEFAFAMKGPEV
                     FLDACLMTAVCLKEQERYDSASAQLERLLTDPRCQGAKAQAIRYELGLLYEKQQQWQL
                     AATMYEAIPAFHDVPQRLAAIRTVPKPASASAQAFRYAT"
     gene            complement(4437..5399)
                     /locus_tag="VFR82_14175"
     CDS             complement(4437..5399)
                     /locus_tag="VFR82_14175"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEU4502796.1"
                     /translation="MASAKPLVYVSRLLPDPVMAAVRAQYALLHEPSNGSATPTALRQ
                     GLSQAVAAIVTLTDRIDAETLASARELKILANYAVGYNNIDLDAAQQRGIVVTNTPDV
                     LTDATADLTWALLLAAARRVVEGDRLVRSGQWTGWTPTQLLGTAVAGRIVGIVGMGRI
                     GQAVARRAAGFQMQVNYHSHRAVDAPAGAHWVYRDLPELLRESDIVTIHVPLTPETRH
                     LIGAPEFALMRRTAMLINTARGPIVDESALVTALQQGTIAGAGLDVFEDEPKIHPQLA
                     SMQQVVLLPHLGSATLVTRVEMGMICLKNIEAILAGRPAPNRVA"
     gene            5581..8409
                     /locus_tag="VFR82_14180"
     CDS             5581..8409
                     /locus_tag="VFR82_14180"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HEU4502797.1"
                     /translation="MLVMPDTSRSIGADLRRLLKPDQIKDDAPTKTAYAVDASIYRME
                     PQAIALVESEDDIQRVVTYAMSRSVPLTARAAGTNLTGSAIGSGIILDVSRLHRILEV
                     NREERWARVQPGIVLAELNKQLAREGLMFGPDPSSGDMCKLGGMVANNSAGPHTLRYG
                     AVKDNVRSLRVCLASGSWLDAKNFDLDDPALAGVLSSHAPIRETLSLVRTHADVLARK
                     RPNVSKNSCGYNLFGLADGLPHGRLNLPHLFVGSEGTLGVFSEATISLVTKPRATLTA
                     LIHFKRLEDVGDAVPRLLALTPSALEVMDANTLDLIGRATHGIPAAAAATLLAELDAD
                     SGAVDLREAAERMATICGDYPLASEIVVAYDPDRREQLWKARKALYPTLYRFDPRKKP
                     INFVDDVVVRAERISELIRYLEEFFGSQRVPVAIFGHIGNGNAHIVPLLDVNDKGDFD
                     KMVKGYHEIHQTVLDRFEGSICGEHGDGRVRAEYVPAMFGPELYDLFVKVKRSFDPAG
                     MLNPGVKISDRPFTDHIDYTRLSKSCATCAKCNAVCPVYDVFRSEDMSSRGWFEIVTD
                     ARYSYLNSKRVVEACLNCKSCRTACPAGVDVSQLILEKRAEHPNRLAGAIFRFQAKTK
                     QFAVFLKVVARFQPLWDRPLTRRLLDRVSRPILSRLAESARFSPAIVMPKFATRHLRD
                     RYPRLALEPEQSRRSAVAYFHGCAANYFDDGVGDAVIAVLRKHGVQPDLPAQRCSGTP
                     VETYGHRELAKDGARINLQSFTGYDTIVTGCASCTLMLKDYPKLFAGEPEQAAAEAMA
                     RRVRHISEFVVQSPVRPPMAAAGAASTVTYHSSCHLRAAGVTKEPRAILQNLPGVQYV
                     EMPDADRCAGGAGTYLVKDFETSQRIVSRKRQAVAQSGAGVVATSCPACMIQLKTGLP
                     ADVEVKHVAQVLRESYEAGERQTGEPRP"
     gene            8406..8645
                     /locus_tag="VFR82_14185"
     CDS             8406..8645
                     /locus_tag="VFR82_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502798.1"
                     /translation="MRLRRADWLFVVFAVTVVAGVSFLPTPKDRNPMVPRTAEHQAVK
                     SEQQCVGCHKTDGVKPLPARHPKRPDCLRCHARQL"
     gene            8738..9016
                     /locus_tag="VFR82_14190"
     CDS             8738..9016
                     /locus_tag="VFR82_14190"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU4502799.1"
                     /translation="MVKVLVFGLTLRDALGTHEVELDVPQATTVKKLIDAYPDELGAV
                     APFMQQREVLVAVNKKVGSEDSPVKDGDVVKLTHQSKTSYDGVRDIPT"
     gene            complement(9126..9770)
                     /locus_tag="VFR82_14195"
     CDS             complement(9126..9770)
                     /locus_tag="VFR82_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502800.1"
                     /translation="MSQCLPVLLYLAVTVPIALVPTIGLADIAELAPPPPSASFDDVD
                     DMTVQAEVTGQALDFLTTLQRHWGESAERLRTLIAGAIERDQAGTLIYLRNFHDHGVL
                     EGYQFRKGSLTCGHYVMVQGPLNGLNEFIGYYTSLKRALRSKYGEPELDRVVWDNDLY
                     QPLPDYWGVAVMIGHLSYHASWTTIDGTITLGLTGNRYSRLSLESCTTREEAHT"
     gene            complement(9826..>10433)
                     /locus_tag="VFR82_14200"
     CDS             complement(9826..>10433)
                     /locus_tag="VFR82_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HEU4502801.1"
                     /translation="RIQFLRRLGDRDVSTSRGRGGEMGDRDVPATRERNVANPTWPDL
                     SDERLLNTLEDWLGPYLYGITTLERVQRMDLTQPLHALLAWNQQRELDRLAPSHLTVP
                     SGSTIRLDYETPDLPVLAVRLQEMFGCRETPRIANNTVPVLLHLLSPAKRPVQVTRDL
                     ASFWVNGYPAVRKELRGRYPKHHWPDDPLTALPTAKTKRRA"
BASE COUNT         2238 a         2981 c         3183 g         2031 t
ORIGIN      
        1 cgagagcgtc agcaccgcag agggcggttt cttttcaatg cggatcacac gaatttcttc
       61 ggccttgttc gtcgcgagcg ggatcaccgt cgagctgaac agcaacagca ccaaggacat
      121 gaggacggcg aacgtcagga agggtgaggc aatccagggg aggctgattc cgcagctgcg
      181 gatggcggta atctcattgt tgcgcgagag caagccgagc gtcagcagcg tggccatcag
      241 gacggcgagt ggaacgattt ggtacgacac accgggaatt ttgagtgcaa agtaactcag
      301 cacatccagc cacgtcgcat cgaagctcaa gaagcggcgg actttttcaa agaaatcgat
      361 gaccagataa atggtcatca ggcccgcaaa acacatcagg aaaatcttcg cgtattctcg
      421 cagcaggtat cgaaagagaa tgggcatacg cggctattgc ttgctcatgc gatagaagct
      481 cacgagtgta atgaccaaga agacgctgtt cgggagccag gcgccggcga acggagggat
      541 ggccgtcgct gtgaccagaa actcacaagc cacattcaac acgtagtagg cgatgatcac
      601 gagcacccct acggcgaacc cgccgatacg gcccgaccgt ttcgagacga ttccgaccgg
      661 aagaccgagc atgcacagga tgagcgaagc cgttggaaag gccatgtcct tatagtactc
      721 ggtgaggcgc cggagggccc atgggtcgcg ccactgtgag gcgttcaacc gggctaccaa
      781 ttgatcgtac gacggccgtt cctccgtcga tgcatatccg ctttggttaa gggaaagctt
      841 gaggtcatag ctggaaaagg aggccagttg gtactcatcg atttcatccg gcttggtatg
      901 aatcacgccg tcctgcagcc gtaaggccac ttggtcgctg gcgctgtcga tcagcacgcg
      961 gtagtcctgg gcgacgatca cgcgaggctc tgaggcaatt cgtccgtccg aaataaagat
     1021 gccgccggaa ggctgttcgc ctttgtccga gacgtaaatc gtcatgcccg gaatcggttc
     1081 ggtgaacgat ccgcgttcca gcgccaacac gagttgatcc cgcagcaaac tcagcgccac
     1141 ctttttcaga ttgatcgaac tccagggctg cccccattgc gcgagccaga gcgtcatacc
     1201 gcacaccagc agggcgaaca gcatcacagg gcgtgccagc cgcaacaggc tcaagccggc
     1261 ggcgcgcatg gccaccagct ccttgtccag cgacagtctg ccaaaggcgg tgatggacgc
     1321 gatgatcccg gcaatcggga gggtcagcac gagaaacgac ggaagcaggt gggcaaacac
     1381 gttgaggacg gcccataatc ctacgccctt ggtgacgaga agctcgacca gccgaagcag
     1441 ctcgcgggtc agcatgatga agcacagcat gccgaggctg acgctgaagg gatagagcaa
     1501 ctcggtgaaa atatagcggt ctagcagcgt acgaagcgtc atgagcggcg gctcgctccc
     1561 gatgtcggac actatagggg actggtacgg gcttgtccac acgggcgggc aggacccctt
     1621 cggtgctgag agcggcttga caagattaga atgcgcatgg tacatgctgc gcgcgcgatt
     1681 tgtaaccaag atgggtctcg tgagtacgtc ttttcttctg cacagagccc gcccatccgt
     1741 ccccggttct catattccat tgttcattca ttattcatgt agtgtgcgtt aactgggttc
     1801 gggcccggct cacgcaggag tgcgtggccg ttgaggaaag gagggttttg tgaagacaaa
     1861 aggtacggtg aagtggttca acgatcggaa agggtttgga ttcatccgtc tggatagcgg
     1921 agaggatgtg tttgtgcatt actccgcgct tcagggggaa ggattcaagt cgttgaagga
     1981 gggagaaaac gtcgaatttg atatcgtgca aggcgccaag ggaccacagg ctgccaatgt
     2041 catgaaaatg tcgatcgccg cgtcctaacg gtctacgact ttaagctgca tcatgcgcaa
     2101 ggcctgccgg aaacactccg gcaggccttt cgtgttttag cgagattgat gcagaccttt
     2161 tagggcattt cgttgacaaa aactattgat actgttagcg tttgttgact attggaggtt
     2221 ctcttgggca tcgaccgcag taaggtcctg cacagcgccc agctctttgc ttcaaaaggt
     2281 cagttcgacg ccgccatcag cgaatggaag aaactctcgg ccgagtcacc gggcgacggg
     2341 agtattcaca attccatcgg cgaactgcat ctgaaacgca actcgaccgc tgacgccgtc
     2401 gcttccttta tccaggccgc caatgccttt cgtgccgaag gagcgaccct caaggcgatc
     2461 gccaccttta aaaaagtgct taagctcgac ccgtcgcggt acgacatgta ccggtttctc
     2521 ggcgatttga acgctgagag aggactgctg agcagcgcag tccaggatta cttgacgttg
     2581 ggtaaacact atctgaagga acgcaagacc aaagaagccc tcgatatcta caagaaaatc
     2641 gtcagtcagg acccctcgaa tctcgatgcc cagcagcgcg tggccgaact gtgcgtgcag
     2701 gaaaacatgc aagatgaggc cacgagagtc tacctgcagc tcgggcgaga gcgctccggt
     2761 caacaacggt atgccgaagc caaagaagcc tacgaatccg tgttgcgcat cgatccttcg
     2821 aacagcgagg cgcagcagtt cattgaacat tacaaaaaaa ccggcgggac gccgccaccg
     2881 cgaacgacga agccgggatc ggcgccggcg gcccataccg gtgagccgac ggacttgttg
     2941 gcggaagcca gccggcgcat cgaagaaaag caatacgccg gtgccgaggc catcctcaat
     3001 cagatgctca cgaaagaacc gggcaatccg caggtctgcc aactcttggc gcggttgcac
     3061 cttcagcaag gacagttgca ggtcgccctt ggtgaatacc gctttctcgc gggggcagcc
     3121 ttacgtgcgc aggactatcc acaggccgaa ttgttgatcc gagaattttt gacggttgaa
     3181 cccaactccg tgccgcttct cgaattgcaa ggtgagctgt acgaagagaa gggcgagatc
     3241 gagtcggcga tccagcacta tgggaaagcc atcgaagtgc tcttgaagac tccagaaccg
     3301 ggcatgccca cgttgcacga ggaaattttc gagaaggtgc agtcgctggc gcccgacagt
     3361 cccatggcca agcggctggc agcattgatg cgtggcgacg ccaccgtgga acctgccgcg
     3421 gcacccgccg ctgccacgat cggaggcttt tcgatcgaag gggcgacgcc cgacgatgcc
     3481 tggaacatga cctcggccat caagccgact caggaccggc atctgacagg gcagggcgca
     3541 cccgcaagtc tgccgccatc gtcgccgagc acgccggaag tcttttccgt tgagaacgcc
     3601 actccggatg atatgccaag tctctcgcac aaggctcccg agccggtcgc ctccagccaa
     3661 ccggctcctt caatctcgga acagtttcag gcctactcgg agtcggggca gtgggctcaa
     3721 gccgaacagt ggctcggcac ggtcgtcagc gcacagcccg aagatccgca cccacgggaa
     3781 ctcctcggtc aggtgctcga gcgaaaaggc gatggagccg gagcggccct tcaatacgcc
     3841 cgagccctcg aactgatgtc agtgggtcaa gactcggttg tgaacgggcc gattcgtcac
     3901 ctctacgaca aagtgaaaga actggcgccg gctagtccga ttgtcgcaag actgtctgca
     3961 ttgttggcgc cggctgctcc ggtcgatgac gtcgatgccg acacccacta taccctcggg
     4021 gtggcctata agaacatggg cctcatcgag gaggcgaaag aggagttcgc gtttgccatg
     4081 aaaggccctg aggtgtttct agatgcctgc ctgatgacgg cggtctgctt gaaggaacag
     4141 gaacgatacg attcggcaag cgcacaactc gagcggctcc tcaccgatcc gcgttgtcag
     4201 ggtgccaagg ctcaggccat tcgctatgag ctcgggttgc tctacgagaa gcagcagcag
     4261 tggcagcttg ccgcaacgat gtacgaggcg attccagcct ttcacgacgt gccacaacgc
     4321 ctggcggcca tccgaacggt cccgaagcca gcctctgctt ccgcgcaagc ctttcgttac
     4381 gcgacgtaat agtacgctgt ccgccccttc ccttgatccg ccaaattgcg aatctctcac
     4441 gccacgcggt tgggcgccgg ccttccagcc aggatagcct cgatgttctt gagacagatc
     4501 atgcccatct ccacccttgt gaccaaggtt gcggagccga gatgcggcaa taataccacc
     4561 tgctgcatgg acgcgagttg cggatggatc ttcggctcgt cctcaaatac atccagcccg
     4621 gctccggcga tggtcccctg ttgcaaggcc gtgacgaggg ccgattcatc aacgatcggc
     4681 cctcttgcgg tattgatcaa catggcagta cggcgcatga gtgcgaattc tggagcaccg
     4741 atcagatgtc gcgtctcagg cgtcaagggc acatggatgg tgacgatgtc tgattcgcgg
     4801 aggagttccg gcagatcacg atacacccag tgggcccctg ccggtgcgtc aaccgcgcga
     4861 tgtgagtgat agttcacctg catctgaaag ccggcggccc gccgcgcgac cgcctgaccg
     4921 attcgtccca ttcccacaat gcccacgatg cgcccggcga cggcggtgcc cagtagttga
     4981 gtgggtgtcc acccggtcca ctgtccgctg cgaaccagtc gatctccttc gacaacccgc
     5041 cgcgcagcag ccaacagcaa ggcccaggtg aggtcggcgg tcgcgtccgt gagcacatcc
     5101 ggggtattgg tgaccacgat gccccgctgc tgagccgcgt ccagatcaat gttattgtag
     5161 cccaccgcat agttagccag gattttcaac tcgcgtgccg atgccaaggt ttcggcgtca
     5221 atccgatccg tgagtgtgac gattgcggca acggcctgcg agaggccctg tctcaacgcg
     5281 gttggagtag ccgacccgtt cgacggttcg tgcaacaggg cgtactgtgc tcggacggcg
     5341 gccatcacgg gatccggcag gagtcgggag acataaacga gaggttttgc tgaggccatc
     5401 ggtgcgctat cttaacgagt tgttcgagaa accgaaaccg ggggcgcaaa ctgatcgccg
     5461 cgtgatcgcc gggctgctgg catttcatga tcagctcggg gtgcgtcaac gaggagcgac
     5521 tggcaggcgc gatgacatga ggtagcagaa cttttctcct ccctgttaga atgccgcgca
     5581 atgctggtaa tgcctgatac ttcccgctcg atcggagccg atctccgtcg actcctgaag
     5641 ccggaccaaa ttaaagacga cgcgccaacc aagacggcct atgcagtcga tgcgagcatc
     5701 tatcgcatgg aaccgcaggc cattgcctta gtggagtcgg aagacgatat acagcgtgta
     5761 gtgacatacg cgatgagccg cagtgtcccg ctcacggccc gcgccgccgg gaccaattta
     5821 accggttccg ccatcggatc gggcatcatt ctcgatgtgt cccgcctgca tcgaatcttg
     5881 gaggtcaacc gagaagaacg atgggcccgt gttcaaccag ggatcgtcct cgccgagctc
     5941 aacaagcagc tggctcgcga ggggctgatg ttcggtcctg atccgtccag cggcgacatg
     6001 tgcaagctcg gcggcatggt agccaacaac tcggccggtc cccacacctt gcggtatgga
     6061 gcggtgaagg acaatgtgcg gagcctgcgt gtgtgtctgg catccggctc atggctggat
     6121 gcaaaaaact tcgacctcga tgatccggcg cttgccggcg tgctgtcctc ccatgccccg
     6181 atccgtgaga ctttgtccct tgtccgaacc catgcggatg tactcgcgcg gaaacggccg
     6241 aacgtgagta aaaacagctg cgggtataac ctcttcggac tggccgatgg cttacctcat
     6301 ggccgcctaa atctgccgca tctcttcgtc ggcagcgaag gcacattggg agtgttcagc
     6361 gaggccacga tcagtctcgt gaccaagccg cgcgccacgc tgacggcctt aattcacttc
     6421 aagcgcctcg aggatgtcgg cgatgccgtt ccgaggctcc tcgcactcac cccaagcgct
     6481 ctggaagtga tggatgccaa cacgctcgat ctcatcggcc gagcgacaca tgggataccg
     6541 gctgcggcgg ccgcgacgtt gctggccgag ctcgatgcgg acagcggggc ggtcgatctt
     6601 cgggaagccg ccgaacggat ggcgacgatc tgcggtgact atccgctcgc ctccgagatc
     6661 gtcgtggcct acgatcccga ccggcgcgag caactgtgga aggcccgcaa agccctgtac
     6721 ccgaccttgt atcgattcga tccccgtaag aagccgatca acttcgtcga cgatgtggtg
     6781 gtgcgcgcgg aacggatcag cgagttgatc cggtatctgg aggaattttt cggaagccag
     6841 cgcgtgccgg tcgcgatttt cggtcatatc ggcaacggca atgcgcatat cgtgccgctg
     6901 ctcgatgtca acgacaaggg cgacttcgac aagatggtga agggctatca tgaaatccac
     6961 cagaccgtcc tggaccggtt tgaggggtcc atctgcggag agcatgggga tggtcgggtg
     7021 cgtgccgagt atgtgccggc gatgttcggg ccagagctgt atgacctgtt cgtgaaggtc
     7081 aagagaagtt ttgatcccgc cggcatgttg aatcccggcg tgaagatcag cgacaggccg
     7141 tttaccgacc acatcgacta tacgaggttg tccaaatcct gcgcgacctg tgcgaagtgc
     7201 aatgccgttt gtcccgtgta tgatgtcttc cgctccgagg atatgagctc gcggggttgg
     7261 ttcgagatcg tgacagatgc ccgatacagt tacctgaact ccaaacgggt ggtggaggcc
     7321 tgtttgaact gtaaatcctg tcgaaccgcc tgcccggctg gcgtcgatgt ttctcaattg
     7381 attctggaga agcgcgcgga gcaccccaat aggcttgcgg gagccatctt ccggttccaa
     7441 gcgaagacaa agcagtttgc cgtgttcctc aaggtggtgg cgaggttcca acctctctgg
     7501 gatcggccgc tgaccaggcg actgttggac cgagtgagcc ggccgattct cagccggctg
     7561 gcggagagcg ccagattctc gccggccatc gtcatgccaa aattcgccac tcggcacttg
     7621 cgcgaccgat atccccggtt ggcgttggaa ccggagcaga gccgccgctc ggccgtggcc
     7681 tatttccatg ggtgcgcggc caattatttc gacgatggcg tcggggatgc cgtcatcgcg
     7741 gtcttacgca agcatggcgt ccagccggat ctgcctgcgc aacgatgctc gggaacgcct
     7801 gtggaaacct acgggcatcg ggaattggcg aaagacggcg cgcggatcaa tttgcaatcg
     7861 ttcaccggct acgacacgat cgtcaccggc tgcgcctctt gtacgttgat gctcaaagac
     7921 tatcccaaac tttttgcagg cgaacccgaa caggctgctg ccgaagccat ggcccgccga
     7981 gtgaggcaca tttctgaatt cgtcgtgcaa tcaccggtcc ggccgcccat ggctgccgcc
     8041 ggcgctgcga gcaccgtgac ataccattcc tcgtgccatc ttcgagcggc gggggtgacg
     8101 aaagaacctc gcgcgatttt gcagaatctt cccggcgtgc agtatgtcga gatgccggat
     8161 gcagaccgct gtgcgggcgg agcgggtacc tacctggtca aggatttcga gacatcccaa
     8221 cggattgtgt cgcgcaaacg gcaagcggtc gctcagagcg gcgccggtgt cgtggcgacc
     8281 agttgtccgg cctgcatgat tcagctcaag acgggcttgc cggcagacgt ggaagtgaag
     8341 catgtggcgc aagtcctgcg ggagagttat gaggcaggag agcggcaaac cggcgagcct
     8401 cgcccatgag attgcgcagg gctgattggc tgttcgtcgt attcgccgtc acagtggtgg
     8461 ccggcgtctc ctttctgccg acgccgaaag accgcaaccc gatggtccca cggacggccg
     8521 agcatcaggc agtcaagtcg gagcagcagt gtgtcgggtg tcacaagacc gacggggtga
     8581 agcccttgcc ggcgcggcat cccaagcggc cggattgtct gcgctgtcac gcgcggcaac
     8641 tgtaagcggg aggcatgctg agtgaagagg ctgaagttca acggatgact cgaggagacc
     8701 acgaaccacg agaagtcgac agaggagtgt acgccgcatg gtaaaagtgc tcgtatttgg
     8761 tttgacgtta cgtgatgccc tgggaacaca tgaggtcgag cttgatgtgc ctcaggcgac
     8821 gacggtcaag aaactgatcg atgcgtaccc agatgaacta ggcgccgtcg cccctttcat
     8881 gcagcagcgt gaagtgcttg tggcagtgaa caagaaggtt gggagcgaag actctcctgt
     8941 gaaggacggc gacgtcgtca agctgaccca ccaatcgaaa acttcgtatg atggtgtccg
     9001 cgacattccc acctgaacat ggcacatgcg taggaagagt gtctggcgcg gccggccatg
     9061 gaaacgacgc gtagccttat ggaaacgacg cgtagccttt atgtgcacag gctgcgtgac
     9121 gagtgctagg tgtgtgcctc ttcacgcgtg gtgcaggatt ccagcgagag tctgctgtag
     9181 cggttaccgg tcagtccgag tgtaatcgtc ccatcaatcg tagtccagga cgcatggtag
     9241 ctcaagtggc cgatcatgac ggccactccc caatagtcgg gaagcggttg gtacaaatca
     9301 ttgtcccaca cgactcgatc gagttccggt tctccatact tcgagcgcaa ggcccgcttg
     9361 agtgaggtgt agtagccgat gaattcgttc aagccgttga gcgggccttg caccatcaca
     9421 tagtggccac aagtcaacga tccctttcgg aactgatagc cctcgagcac gccatgatcg
     9481 tgaaaattgc ggaggtagat gagcgtgcct gcttgatcgc gttcgatggc gcctgcgatg
     9541 agcgtcctca gccgttctgc actctctccc caatgccgct gaagtgtggt gagaaagtcg
     9601 agtgcctgcc cggtgacttc ggcctgaacc gtcatgtcgt cgacatcatc gaaggatgcc
     9661 gagggtggag gaggcgcaag ctctgcgatg tctgccaggc cgatcgttgg aacgagcgcg
     9721 attggaacgg tcacggcgag gtacaggaga acaggtaggc attgggacat gcatcgcacg
     9781 ataagatgcc caaccgtcct tggtctatcc tacgaaaggg cgatgctacg cgcggcgctt
     9841 tgttttggct gtcgggagtg cggtaagcgg atcatccggc caatgatgtt tgggataacg
     9901 tccccgaagt tctttgcgga ctgcgggata gccgttgacc caaaaactgg cgagatcacg
     9961 ggtcacctgc accggccgct ttgcgggcga caagagatgc aagagtacag gaaccgtgtt
    10021 gttcgcgatc cgaggggtct ctctacagcc gaacatttct tgaaggcgca cggccaggac
    10081 aggaagatcc ggcgtctcgt agtcgagcct gattgttgat ccgctgggta cggtgagatg
    10141 cgaaggcgcc aaacggtcta actcgcgctg ctgattccat gccagcagcg catggagcgg
    10201 ttgcgtcaga tccatgcgtt gcacgcgctc caacgtcgtg atcccgtaga gatagggacc
    10261 cagccagtct tccagtgtgt tgagcaaccg ctcgtccgac aagtccggcc atgtcgggtt
    10321 cgcaacgttt ctttccctcg tcgccgggac gtcgcggtca cccatctcac ccccccgccc
    10381 tcgcgacgtg gaaacgtcac ggtcacccag gcgccgcaag aactgaattc gcc
//
LOCUS       DASTQY010000441         3446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1341944, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000441 DASTQY010000000
VERSION     DASTQY010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341944"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFR82_14205"
     CDS             complement(<1..253)
                     /locus_tag="VFR82_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502802.1"
                     /translation="MPKPDRFVSRYRASERWRHDPRVLRCRAAQDERGVALLLALLVL
                     ALLVALILEFDTDARREYRDAAAFRDNFKATVLTRAAVQA"
     gene            complement(240..788)
                     /gene="gspM"
                     /locus_tag="VFR82_14210"
     CDS             complement(240..788)
                     /gene="gspM"
                     /locus_tag="VFR82_14210"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEU4502803.1"
                     /translation="MIATLQERWRHMASRERLIVASGGAVVLVSLLFLLVVDPLLTRI
                     DKLERQAVRKSKERVELSALASDYAAKQARITKLEQRLPASEGQFSLLAFMEDAAASV
                     QIRDRIVGMQPQQATTQQGYQEMAVDLRLDGVQLPQLLALLVALEQAPYDVQVHHLQI
                     KPKYDNPINLDATLRIVSYAKA"
     gene            complement(785..2359)
                     /gene="pilM"
                     /locus_tag="VFR82_14215"
     CDS             complement(785..2359)
                     /gene="pilM"
                     /locus_tag="VFR82_14215"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEU4502804.1"
                     /translation="MVAECVGLDLGQTAIKAVRFRRRLSGRESVEYFHLPLPYGKPVQ
                     AEPARLAGHLRNFLWQHGLYGSGDIVTALPCQDLFIRTLSFPFRDSAKLEQVVPFEVE
                     NLIPMALDEVAMGSVVLPAKGTGAGATQKLKGADVLITAAPKDKVAEHLKFLATADLK
                     PAAVGVDGMALYSVTKFLQEEGAQVPGDLAVIDVGATKTTLCLIHDGRPVVLRTVLWG
                     SNHLTHALAVRYACTFAEAERRKRAMGVQELETWLEPLLKELRVSLHAYEGTAHQRLS
                     HCWVSGGGSKLRELSGHVAHELGLTPVGPRQGFGANCPRAFSIAFGLAIHPKIVRPRW
                     KTRAIGSDVALDLAATDGSAARSSKESQQDRRLALWGGLILGVLVLIDLSVRVYVKES
                     TLTQLKQALQTQFAQSFGEGGPPGQELDVARSRVAQLDKSLAVIDRSHHSVLLNLSDL
                     AKQLPTGIPLTIRELTVEGASVHLEGETNSFDSIEKIKQAFTSNQLFQAVSVTDTRVG
                     ATPSQVVFRLTYTVQR"
     gene            complement(2398..3291)
                     /gene="gspN"
                     /locus_tag="VFR82_14220"
     CDS             complement(2398..3291)
                     /gene="gspN"
                     /locus_tag="VFR82_14220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HEU4502805.1"
                     /translation="MARLSWTPTIALKKEPLAWVLGGLGSFAVFLFLTFPFGTLQARV
                     LSELVKVTGGEIRAAEWSPGIPIAVEWRDLTWTKPGTSSIPIQLMRLNVGVMGLLMGP
                     KTVDALIQFPGGGQAAKATGTVTVSSWAFEGPVSLKARAQHIDLATVIKPYVTRGLLE
                     ADVAQRWENHGKEGIAFKGNGSWKAEIKELMLERLPVGPVTIPPLTFTRVAATLSCHD
                     AVCDVTDFKGDGPDGTITALGRLLLRQPLQSSVLDLTVTVVPGAGWAQKAGTLPIPPL
                     PPGTPLTLKLAGSVANPKLTL"
BASE COUNT          724 a         1015 c         1019 g          688 t
ORIGIN      
        1 cggcctgaac cgcggcccgg gtgagcactg tggctttaaa attgtctcgg aaggcagcgg
       61 cgtcacgata ttcgcggcgg gcgtccgtgt cgaattcgag gatcagggcg acgaggagtg
      121 ccaacacgag aagggcaaga agcagggcta cgcctcgttc atcctgcgca gcgcgacacc
      181 gaaggacacg ggggtcatgc ctccatcgct cagaagctcg atagcgactg acaaaacgat
      241 caggctttgg cataggacac gatacggagc gtcgcgtcca agttgattgg attatcgtat
      301 ttaggcttga tttggaggtg atggacttgg acatcgtatg gtgcctgctc aagagcgacc
      361 aacaaggcca acaactgcgg caactggact ccgtccaacc gcaggtccac ggccatttcc
      421 tgatagcctt gctgggtcgt cgcctgctgc ggctgcatcc ccacgatgcg atcccgaatc
      481 tgcacagagg ctgcagcgtc ctccataaag gccagaagag agaattgtcc ctcgctggcc
      541 ggaagtcgtt gctccagctt ggtgatccgc gcttgctttg cagcatagtc ggatgccaac
      601 gctgacaact cgacacgctc tttcgatttc ctgaccgcct gccgttcaag cttgtcaatc
      661 ctggtcagga gtggatcaac gacgagcaaa aacaggagac ttaccaggac aacggccccg
      721 ccgctggcca cgataagccg ctctcgcgac gccatatgcc gccagcgttc ctgtagagtg
      781 gcgatcaccg ttgcaccgtg taggtgagcc gaaagacaac ctggctcggg gtggcgccaa
      841 cccgcgtatc ggttaccgag accgcctgaa acagttggtt agacgtgaag gcttgtttga
      901 tcttctcaat ggaatcgaat gagtttgttt cgccttcgag gtggaccgaa gctccttcca
      961 cagtcagttc ccgaatcgtg aggggaatac cggtaggaag ctgcttcgcc aaatccgaca
     1021 gattcagcag cacgctgtga tggctccggt cgatgaccgc caagctcttg tcgagctggg
     1081 cgacgcggga tcgtgccacg tcgagttcct gcccgggcgg cccgccttcg ccgaacgatt
     1141 gggcgaattg cgtctgcagc gcctgcttca gttgcgtcaa tgtcgattcc ttgacgtaga
     1201 cacgaacgga gagatcgatt agcaccagga ctccgagaat caatccgccc cacagcgcca
     1261 gacgccgatc ctgttgtgat tctttgctgc tgcgagccgc actcccgtcc gtggcagcca
     1321 gatcgagcgc cacgtctgag ccgatggctc gagtcttcca gcgaggtcgc acgatttttg
     1381 gatgaatcgc caatccgaag gcgatggaga aggcgcgtgg gcagttggct ccaaagcctt
     1441 gacggggacc gacaggggtc aatcctaatt catgtgcgac gtgaccgctc aactcgcgca
     1501 gtttggagcc accgccggaa acccaacagt gcgagagccg ctgatgcgcc gttccttcgt
     1561 acgcgtggag cgacacccgc agctccttga gtaaaggttc gagccacgtc tccagctctt
     1621 gaacccccat ggcgcgcttg cgccgttcgg cctctgcaaa ggtacaggca tagcggacgg
     1681 ctaacgcatg ggtcaggtgg ttgcttcccc aaagcacggt ccggaggacc acgggacggc
     1741 cgtcatgaat caggcaaagt gtggttttgg tcgctcctac atcgataacg gccaagtcgc
     1801 cagggacctg cgcgccttct tcctgtagaa acttcgtgac agagtacagg gccatgccgt
     1861 cgaccccgac ggctgccggt ttgagatcag ccgtagcgag aaacttgaga tgctcggcca
     1921 ctttgtcctt gggtgctgcg gtgatgagta cgtcggcccc tttaagtttt tgggtggctc
     1981 ccgcgcctgt gcctttggca ggcagcacga cgctgcccat ggcgacttca tcgagcgcca
     2041 tcggtatgag attctcgact tcaaacggaa cgacttgctc gagttttgcc gagtcacgga
     2101 acggaaacga gagggtacga atgaagagat cctggcaggg cagcgccgtt acgatgtcgc
     2161 cgctgccata cagcccgtgt tgccacaaga aatttcgcag gtgtccggca agacgagccg
     2221 gctcggcttg gacaggcttg ccatagggca gcggcagatg gaaatactcg accgattcgc
     2281 gtccggacaa gcgccgacga aatcggacag ccttaatggc ggtctgcccg agatcgagtc
     2341 cgacacactc tgcgaccata acctacgcac gctccggtat agagataagc ttgtatacta
     2401 cagggttagc ttgggatttg cgactgatcc cgcaagtttg agagtcagag gggtccccgg
     2461 gggaagggga ggaatcggca atgtccccgc cttctgcgcc catcctgcac ctggtacaac
     2521 cgttaccgtg agatccaata cgctcgattg aagcggttgc cgaagcagga ggcgacccag
     2581 ggcggtgatg gtcccatctg ggccgtctcc cttgaaatcg gtcacgtcgc agacagcgtc
     2641 gtgacagctc aaggtcgcag ccacccgggt gaacgtcaga ggtggaatgg tgaccggccc
     2701 aacaggcaga cgctcaagca tcagttcctt gatctccgcc ttccaggaac cgttaccttt
     2761 gaacgcaatg ccttccttgc cgtgattctc ccatcgttgt gcgacgtcag cctctaacag
     2821 tcccctggtg acatacggct tgatcacggt cgcgagatcg atgtgttgag cgcgggcctt
     2881 gagcgaaacc gggccttcaa acgcccagga cgagacagtg accgtacccg tcgccttcgc
     2941 cgcctgtccg ccgcccggaa attggatgag cgcgtcgacc gtttttggtc ccatcagcag
     3001 acccatcaca ccgacattga gccgcatcaa ttggattgga atgctgctcg tacccggctt
     3061 ggtccaggtc aggtcgcgcc actcgacggc gatgggaatg ccgggagacc actcggccgc
     3121 tcggatttct ccaccggtca ctttgacgag ctccgacaag actctcgctt gcagggtacc
     3181 gaaggggaag gtgaggaata agaatacggc aaagcttccg agtccgccca acacccaggc
     3241 caagggttct tttttaagcg caatcgtcgg ggtccaggac aggcgagcca ttacgacttc
     3301 actccaatcg gaacccattg gcggaaggtg cgcacctcgt cattgtcctc cagcaacatc
     3361 aattcaatca gcatggccgc tgggggagcg gttcttgagc gaccgtccca ctcgtccagc
     3421 caggcattgg ccattgagtc gtaata
//
LOCUS       DASTQY010000442         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_317650, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000442 DASTQY010000000
VERSION     DASTQY010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317650"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VFR82_14225"
     CDS             <1..641
                     /locus_tag="VFR82_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEU4502806.1"
                     /translation="ITGLSIEQLTDANARSQFKRAIVVNALLFIAGFSVVFIAFGASA
                     SFVGQLLMTYQEHIRRIGGLLIVIFGLYLLGILNINFLQMEHRFQFRNRPVGYLGSFL
                     IGIAFAAGWTPCVGPVLGTILLYASTTDSLLDGVFLLTSYSLGLGLPLFLTALGVDRF
                     LAYFKQARAYLWGVSTVSGVLLVIVGVMIYANSLTMITSFLERHGIGWYLGQ"
     gene            complement(853..1794)
                     /gene="bamD"
                     /locus_tag="VFR82_14230"
     CDS             complement(853..1794)
                     /gene="bamD"
                     /locus_tag="VFR82_14230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEU4502807.1"
                     /translation="MKINLLQTDLLSAAHGLRLALGLAAICLLIACSSSPKNGDATSK
                     KALSGTDESIFLGDTIEKNYDPNVIMKRGEAFFEKEEFAEAIVEYTHFLDLHRSHMLA
                     PYAGFRIGESHMKRAKGIDRDPEPIQKAIDSFERLRRDYPGSRYDSEAVDKIQECHDL
                     LAQMHLFVGQFYYRRQSYLAAAHRFEQIMKIYPDKSVAPDALYFLALSYHDLGADDWA
                     SEKLTLLAEKYPNSTHSNDGAKLLAKIIKSQPSVLVAKKPEAAVPVSEPALAAQESPR
                     SVPAGGVTPQVANGLMRLPSATAMGQGFVNCRLGAWC"
     gene            1918..>2614
                     /locus_tag="VFR82_14235"
     CDS             1918..>2614
                     /locus_tag="VFR82_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HEU4502808.1"
                     /translation="MIRSRIIGTGSYLPERVVSNREAGAALGIDPETVMQLTGIRERR
                     WAEASQASSDLAVCAARNALQAAGLPLGAIEVIVVSTTSPDMAFPSTACHLQRKLGCA
                     SVPAFDVAASCSGFLYGLSMADAMVRSGQAKTCLVVAAEVKSRMLDPSDSDTALLFGD
                     GAGAAIVHGEPDADQPSRGLLGIRLYSDGARHELIRIRAGGSRRPASHETLDGQEHSL
                     RMQGAPLFRLAVRH"
BASE COUNT          493 a          730 c          758 g          633 t
ORIGIN      
        1 acatcacagg tttatcaatc gagcagctga ccgatgccaa tgcgcggtca cagttcaaga
       61 gagcgattgt ggtgaatgct ctgttgttca ttgccggctt ttccgtcgtc ttcattgcct
      121 tcggggcctc cgccagtttc gtcggtcagc tcctcatgac ctatcaagag cacatccggc
      181 gtattggggg tctgctgatc gtcattttcg gcctctatct gctcggcatt ctcaacatta
      241 attttttgca gatggaacac cgcttccaat tccggaaccg gccggtggga tatctcggtt
      301 cgtttttaat tggaatcgcg tttgcagccg gctggacgcc ctgtgtcggg cccgtactcg
      361 gcacaatctt actgtatgcc agcaccaccg attccttgct cgacggcgtg ttcttgttga
      421 ccagttattc gcttggtttg ggcttgccgc tgttcttgac ggccttgggt gtcgatcgtt
      481 ttctggcgta tttcaagcaa gcccgcgcct acctgtgggg agtgtccact gtcagcgggg
      541 tattgcttgt catcgtcggc gtgatgatct atgccaattc actgacgatg attacgagtt
      601 ttttggagcg gcatggcatc ggctggtatc taggtcagta gctttcatag acccttcccc
      661 atttctctcc atattcttcg acagactggt caaacagggc gtcattctta cccgcccacc
      721 caggtgcacc gagacgcgcc ctttaccctg gaaggccgca gcaggcgaaa cgccgaggta
      781 tacgtttgtt ggtatgtcga gggattgagc gaagcgagag cgcagccgac ggcctgtttc
      841 accaacttgg ggttagcacc aggcgccgag tcggcagttc acaaacccct gccccatggc
      901 agtggcagat ggaagccgca tcagtccatt ggccacctgt ggagtcacgc ctcctgccgg
      961 aacggaacgc ggactctctt gagccgcaag ggccggttcc gagaccggta cagctgcctc
     1021 cggctttttc gcaacgagca ccgacggctg ggatttgatg attttggcga gcaattttgc
     1081 gccgtcattg gaatgcgtgc tgttgggata cttttccgca agcaaggtca atttctcact
     1141 cgcccaatcg tccgctccga ggtcgtggta gctgagagcg aggaaataca gcgcatcagg
     1201 cgccacagac ttgtcgggat agatcttcat gatctgctcg aagcggtgtg ccgcggcgag
     1261 gtacgactgc cgccggtaat aaaactgacc gacaaacaga tgcatctgag ccaacagatc
     1321 atggcactcc tgaatcttgt cgaccgcttc gctatcatac cggctgcccg gatagtctct
     1381 tcgcaaccgt tcgaacgaat caatggcttt ttggatgggt tccgggtcgc gatcaatgcc
     1441 cttcgcacgc ttcatgtgac tttcgccgat ccggaaaccg gcgtagggag cgagcatgtg
     1501 actgcgatgc agatcgagga agtgggtata ctcgacgata gcttcggcga attcttcctt
     1561 ttcgaagaag gcttcccccc gtttcataat gacgtttgga tcgtagtttt tttcaatcgt
     1621 atcgccgaga aagatcgatt cgtccgtgcc actcagggct ttcttggacg tggcatcgcc
     1681 gttctttggc gagcttgagc aggcaatgag caggcagatt gccgccagtc ctagggccaa
     1741 tctgaggccg tgagcggctg atagaaggtc tgtctgtaac agattgatct tcatgagttc
     1801 cggttacaaa gctagcagga gaccctttca aaatcaatct ttcccgctcg ggttgaccgc
     1861 tgcaggcgcg agacctataa tcccgcgcgg ttggtgcaac ccctcggcac ttcagcaatg
     1921 attcgatcca ggatcatcgg aacaggctct tatctcccag aacgcgtcgt ctccaatcgc
     1981 gaggccggtg ctgcgttggg aattgatcct gaaacggtca tgcaactgac agggattcga
     2041 gagcgccgat gggcagaggc gtcccaggcc tcctccgatt tggcggtgtg cgccgcacgc
     2101 aatgcgctgc aggcagcagg gcttccgttg ggggcaatcg aggtcatcgt cgtttcgacc
     2161 acctctccgg atatggcctt cccttctacg gcctgccacc tgcaaaggaa gctaggttgt
     2221 gccagcgtgc cggcgtttga cgtcgccgca tcgtgttccg gattcttgta tgggctgtcc
     2281 atggccgatg cgatggttcg gagcggccag gcgaagacct gccttgtcgt ggcagcagag
     2341 gtgaagtcgc gcatgctcga cccaagcgac tcagacacgg cgctgctctt tggcgacggt
     2401 gccggtgcag ccattgtgca tggtgaaccg gacgccgatc agccgagccg cggactcttg
     2461 gggattcggc tctattccga tggcgcgcgg catgagttga ttcgcattcg tgccggcgga
     2521 tcccggaggc ctgcaagtca cgaaacactc gatggccagg agcactcctt gcggatgcag
     2581 ggtgcgccgt tgttccgatt ggccgtgagg catc
//
LOCUS       DASTQY010000443         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_595386, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000443 DASTQY010000000
VERSION     DASTQY010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595386"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFR82_14240"
     CDS             complement(<1..306)
                     /locus_tag="VFR82_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU4502809.1"
                     /translation="MGKKMKAALAKVEPRPYALREAVALVKGAAYAKFDESVDLAIRL
                     GVDPKRSDQMVRGTTSLPHGTGKKLRVLVFAKGEKEQEARQAGADFVGSDDLMERIKG
                     "
     gene            complement(330..755)
                     /gene="rplK"
                     /locus_tag="VFR82_14245"
     CDS             complement(330..755)
                     /gene="rplK"
                     /locus_tag="VFR82_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU4502810.1"
                     /translation="MAKEVSAQIKLQIPAGKANPAPPVGPSLGQHGVNIMEFCKQFNA
                     KTQKEGDSIIPVIITVYKDRSFTFIMKTPPASDLLKKAAGIIKGSGVPQKDKVGKITR
                     KQLTEIAQKKMADLNAADLEGAVKIIEGTARSMGVVVQG"
     gene            complement(768..1343)
                     /gene="nusG"
                     /locus_tag="VFR82_14250"
     CDS             complement(768..1343)
                     /gene="nusG"
                     /locus_tag="VFR82_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU4502811.1"
                     /translation="MSEAAPTNDPKVGMGKNWYVIHTYAGFEGRVKTSLLERANQMAL
                     TEKMGQVLVPTEDVIEIKDGKRRTSRRKFFPGYVLVELETPVTDETLQLIKETPKVTG
                     FVGGGAQPTPLTNEEVAELLKQVDAGSAGPREQVKFIKTDNVRIVDGPFLGFNGVVDE
                     VDQDHNRLKVMVSIFGRSTPVELGFLQVERI"
     gene            complement(1340..1534)
                     /gene="secE"
                     /locus_tag="VFR82_14255"
     CDS             complement(1340..1534)
                     /gene="secE"
                     /locus_tag="VFR82_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU4502812.1"
                     /translation="MFKRMIDNVKEFLGDVRTELKKVSFPTKAETIGSTTVVIVFCVL
                     MSIYLSVIDSFLSWLVGKII"
     gene            complement(1637..1711)
                     /locus_tag="VFR82_14260"
     tRNA            complement(1637..1711)
                     /locus_tag="VFR82_14260"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1676..1678),aa:Trp,seq:cca)
     gene            complement(1780..1929)
                     /gene="rpmG"
                     /locus_tag="VFR82_14265"
     CDS             complement(1780..1929)
                     /gene="rpmG"
                     /locus_tag="VFR82_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU4502813.1"
                     /translation="MREIIDLACATCKQRNYSTTKNKKNDPDRLERNKFCKFCRKHTP
                     HKEVK"
     gene            complement(1953..>2124)
                     /gene="tuf"
                     /locus_tag="VFR82_14270"
     CDS             complement(1953..>2124)
                     /gene="tuf"
                     /locus_tag="VFR82_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031793.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU4502814.1"
                     /translation="TGVVSLNPGVEMVMPGDNVSVTAELISPIAMDQGLRFAVREGGK
                     TVGSGVVTEILA"
BASE COUNT          424 a          656 c          517 g          527 t
ORIGIN      
        1 gcctttgatc cgttccatca gatcgtctga tccgacgaaa tcggcaccgg cctggcgtgc
       61 ttcctgttcc ttttcccctt tggcaaacac gagcacccgc aatttcttgc ccgtgccgtg
      121 agggagagac gtagtcccgc gtaccatttg atccgaacgc ttaggatcaa caccgagccg
      181 gatcgctaga tcgactgatt cgtcgaattt cgcgtaggcg gcgcctttga ccaacgcaac
      241 cgcttctcgg agcgcatatg gacgcggctc cactttggcg agtgcggcct tcattttctt
      301 acccatcgca gacactcctt gctttggcgt taaccctgga ccaccacccc catgctgcgg
      361 gcagtcccct caatgatctt gacagctcct tcgagatcgg cggcattcaa atcggccatc
      421 tttttttgcg caatctcggt gagctgcttg cgcgtgattt tcccgacctt gtccttttgc
      481 ggcacgcccg aacccttgat gatgccggcc gcttttttca gcaggtcgga ggcgggcgga
      541 gttttcataa taaaagtgaa tgaccggtcc ttgtaaacag tgataatcac gggaatgatg
      601 ctgtcgcctt ccttctgcgt cttggcgttg aactgcttgc agaactccat gatattcacg
      661 ccatgctgac ccaatgaagg gccgaccgga ggggccgggt ttgccttgcc cgccggaatt
      721 tgcaatttga tttgggctga tacttccttt gccatgcacg actctcctta tatacgttcg
      781 acctgcagga accccaattc gaccggcgtt gagcggccaa aaatgctgac cataaccttc
      841 aggcggttgt gatcctggtc cacttcatcg acgacgccgt tgaaaccgag gaatggcccg
      901 tccacaatgc gcacattgtc ggtcttgatg aacttgacct gttcgcgagg accggcactc
      961 cccgcatcga cttgtttcaa caactccgcc acttcttcat tggtcaacgg cgtcggctgc
     1021 gcccctccac cgacaaaccc ggtgaccttg ggtgtttcct tgatcaattg cagcgtttcg
     1081 tcggtcacag gcgtttccaa ttcgacgagg acatacccgg gaaagaattt cctccgggag
     1141 gtacggcgct tgccgtcctt gatctcaatc acgtcttcag tcggaaccag aacctggccc
     1201 atcttttcgg tgagcgccat ttgattggcg cgctcaagca agctcgtttt cacccgcccc
     1261 tcaaaacctg cataggtatg aatgacgtac cagttttttc ccatacccac cttcggatcg
     1321 tttgttggcg cggcttcgct catatgattt ttcccaccag ccaggataaa aacgagtcga
     1381 tcacagagag ataaattgac atcagaacgc aaaacacgat gacgacggtc gtcgatccga
     1441 tcgtctcggc tttcgttgga aacgagactt tcttgagctc cgttcggaca tcgccgagga
     1501 actccttcac gttatcgatc atccgtttga acatcgctct cctaggctga gtactgcgat
     1561 ctcacaacgc cacaggcacc atgggattca tcccgtcgcg cccatcgtga gcgctccggg
     1621 tcgagcaagc tcggtttggc aggggcacta ggatttgaac ccagactctc ggttttggag
     1681 accgatgtgc tgccattaac accatgcccc tccgctacag acatccgagt gtagagtcta
     1741 gcgggctcct ggcccggctc agcgctcaga caaagcactc tactttactt ccttatgagg
     1801 cgtgtgcttc cggcagaact tgcaaaactt atttcgctcc aaacgatcag gatcgttctt
     1861 cttgttcttg gtggttgaat aattccgctg cttacaggtc gcgcaggcca aatcgatgat
     1921 ctctcgcatc tctatctcct gtcctctgct gcctaggcca gaatttccgt gacgacgccg
     1981 gaaccgaccg tcttgccgcc ctcacgcacg gcaaagcgca ggccctggtc catcgcgatc
     2041 gggctgatca attcagccgt cacgctcacg ttgtcgcccg gcatcaccat ctccactccc
     2101 ggattcaacg acaccacgcc cgtc
//
LOCUS       DASTQY010000444         3299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1811129, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000444 DASTQY010000000
VERSION     DASTQY010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811129"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VFR82_14275"
     CDS             <1..568
                     /locus_tag="VFR82_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEU4502815.1"
                     /translation="NRPDLIDPAVLRPGRIDRKIKVARPNREASAQILKVYLRHDLPL
                     DPALLAERGGEQGRAMASLAGDVLERIFKRSDENRLLSVRLRNGQHKVLYRGDLVSGA
                     ILASIVQRAKEKAIDRTIQSGQPAGLLAQDLLDAVTEEFREGEVLPPDDAAEEWLKLL
                     DHHPEQVVGVSSFRRGRQTEERLVNQII"
     gene            616..2142
                     /locus_tag="VFR82_14280"
     CDS             616..2142
                     /locus_tag="VFR82_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HEU4502816.1"
                     /translation="MTRLFGIETEYGIGRDDLDTMDPVVESMELVRAHLTSPFERRWD
                     YASEDPHEDARGFRVSGLAQDKEEAQFSTIDAHRPFSFHEMKSDLVLPNGARFYNDHT
                     HPEYSSPECRTLKDLVAHDRAGERIVQRAADRRNQRLGGTPVQLYKNNTDFHGHSYGC
                     HDNYLVSRSIPFAALVAGLLPFLVSRQVVAGAGKMGIESESGFVPGTYQLSQRADFIE
                     TELSVDTMHNRPILNTRDEPHADVKKYRRLHLIIGDANMCEYATALKVGTTQLVLELI
                     ARGRAPGLELEEPVTAVKQIARDADLKAAVKLKDRRNLSGLDLQECYWEAAQRELAGA
                     DPETDWILCEWGETLRLLIQHREQLVGRLDWVTKQWLLETFIREEQIGWNDPWLASLD
                     LEYHNVNPERGLYRGLEAEGKVWRMTNEADVEEALARGPLDTRGGLRGICVRRFADQI
                     TDMQWERIRFSGGWRPKTLDMGDLFDPMEVRQCIALFEEAASPADALAVWKTRKDLTS
                     "
     gene            2139..2333
                     /locus_tag="VFR82_14285"
     CDS             2139..2333
                     /locus_tag="VFR82_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein UBact"
                     /protein_id="HEU4502817.1"
                     /translation="MTYDMAPERREGPINPMPKSPSPPEDGGGPRRPESGSPDKDNLM
                     KRMKKVDPKQAERYRQRTGQ"
     gene            2404..3177
                     /locus_tag="VFR82_14290"
     CDS             2404..3177
                     /locus_tag="VFR82_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HEU4502818.1"
                     /translation="MGMQGDFFQLLKAQGYQFGAAGHAGNDQEFTTATTILAFKYRDG
                     VLVAGDRRATAGNMVMYDRTDKVLEIDRHSVMAIAGVPATAYEMVRILEHSFKYYRRT
                     QLQELSFEGKLRAVSKLLKENVAAALAGTGAVAPIFAGYDFEQESAKIFFYDILGAEF
                     EGVEYAVSGSGSPTIRGILHYLNTWGDQPLHAMSEDQSAIQALRLLTSAAEFDSATGG
                     VNREANLYPVIKLITREGVRTLPETQLKQKFEASVSRFA"
BASE COUNT          740 a          881 c         1029 g          649 t
ORIGIN      
        1 gaaccggccg gatttgattg accccgccgt ccttcggcct ggccgtatcg atcgcaagat
       61 caaagtcgcg cggcccaatc gcgaagcctc agctcagatt ttgaaggtgt acctgcggca
      121 tgacttgcca ctcgatcctg cgttgcttgc ggagcgcggc ggcgaacagg gaagagcgat
      181 ggcttcgctc gccggcgatg tgcttgaacg gatcttcaaa agaagtgatg agaatcgatt
      241 gctgtccgtc cgactcagaa acggccagca caaagtgctc taccggggcg atctggtcag
      301 cggcgccatt ttggcgtcaa tcgtccagcg ggcgaaggaa aaggccatcg accgcacgat
      361 tcaatccggg cagcccgccg ggctcctggc tcaggatctg ctcgacgcag tcacggaaga
      421 atttcgagaa ggggaagtgt taccgcctga tgatgcagcg gaagaatggt tgaaactgct
      481 cgatcatcat ccggaacaag tcgtcggtgt ctcgtctttt cggcggggcc ggcagacgga
      541 agaacggctc gtcaaccaga tcatctaggc acaggtcctg ccgctccatg tgcgcaactg
      601 tcctatggga gaaatgtgac tcgactgttc ggcattgaaa cggagtatgg aattgggcgg
      661 gacgacctgg acaccatgga tcccgtggtg gagtccatgg agttggtacg ggcccatctc
      721 acgagtccgt tcgagcggcg atgggattat gcgagcgaag atccgcacga agatgcacgg
      781 gggtttcggg tatcgggttt ggcgcaggac aaggaagagg cccaattctc gaccatcgat
      841 gcgcatcgcc cattttcgtt ccacgagatg aagagcgacc tcgtgctgcc gaacggcgca
      901 cggttttaca acgaccatac ccatcccgag tactcgtcac cggagtgccg aacactgaaa
      961 gacttggtgg ctcatgatcg ggctggggag cgcatcgtgc agcgggcggc cgaccgccgc
     1021 aatcagcgac tgggcggcac gccggtacag ttgtacaaaa acaacacgga tttccacgga
     1081 catagctacg ggtgtcatga caactatctg gtgtcccgtt ccattccgtt tgcggcgctg
     1141 gtggcaggac tgctgccctt cctcgtcagc cggcaggtcg tagcgggcgc cggaaagatg
     1201 ggtattgaat cagaatccgg ctttgtccct ggcacctacc agctttctca acgcgctgat
     1261 ttcatcgaaa cagaattgag cgtcgacacg atgcataatc gcccgatttt gaacacgcgg
     1321 gacgaaccgc atgccgatgt gaagaagtat cgccgcctgc acctcattat cggcgatgcc
     1381 aacatgtgcg aatatgccac ggcgttgaaa gtcggcacca cgcaactggt attggagttg
     1441 atcgcaagag gccgcgcacc tggtctggag ttggaagaac cggtcacggc cgtgaagcag
     1501 attgctcggg acgccgatct caaggccgct gtcaaactga aagatcgccg gaacctttcc
     1561 ggattggacc ttcaagagtg ttactgggag gcggctcaac gggagttggc cggtgccgat
     1621 ccggagaccg attggattct ctgtgaatgg ggcgagacgt tgcgcttgct gattcagcat
     1681 cgcgagcaac tcgtgggaag gctcgactgg gtcacgaaac agtggctgct cgagacattc
     1741 atccgtgaag agcaaatcgg ttggaacgat ccctggctgg ccagccttga tctggaatac
     1801 cataatgtca acccggagcg aggactctat cgcggtctcg aggcagaggg aaaagtctgg
     1861 cgcatgacga acgaagcgga cgtggaggaa gccttggctc gcggtcctct cgatacgcgc
     1921 ggcggtcttc ggggaatctg tgtcaggcgt tttgcagacc agatcaccga catgcaatgg
     1981 gagcgaatcc gcttttccgg cggctggcgg ccgaagacac tcgacatggg ggacctgttc
     2041 gatcccatgg aagtgcgtca atgcatcgcg ctgttcgagg aagcggcttc cccggctgac
     2101 gctcttgcag tctggaagac acgaaaggat ctcacatcat gacctacgac atggcacctg
     2161 aacgacgcga agggccgatc aatcccatgc cgaaatcgcc ttctccaccg gaggatggtg
     2221 gggggccgcg gcggccagaa agcgggtctc ctgacaaaga caacttgatg aaacgaatga
     2281 aaaaagtcga tcccaaacag gccgaacgct atcgacaacg aacgggccaa taaatacgtc
     2341 gtgccgccgg aatgttcctg gggtccggac gcagccgcac agttaaccca gcgtaggtga
     2401 agaatgggga tgcagggaga tttttttcag ctgttgaaag cgcaaggcta tcaattcgga
     2461 gcggctggcc atgccggcaa cgaccaggag ttcacgactg cgacaaccat tctggcgttc
     2521 aaatatcgcg atggtgtcct cgttgccggt gatcggcgtg caaccgctgg caacatggtg
     2581 atgtacgatc gcaccgacaa agtccttgaa atcgatcggc acagcgtgat ggccatcgcc
     2641 ggcgtgccag ccacggccta tgaaatggtg cggatcctgg aacattcttt caaatactac
     2701 cggcgcacgc agttgcagga attgagcttt gaaggcaagc tccgtgcggt gtcgaagctc
     2761 ttgaaggaaa atgtggccgc ggcgctggct gggacggggg cggtcgcacc gatattcgcc
     2821 ggctatgact ttgagcagga aagcgcaaag attttcttct acgacattct cggggcggaa
     2881 ttcgagggtg tcgagtatgc cgtctccggg tccggctcgc cgaccatcag aggcattctg
     2941 cattatctga atacctgggg cgaccagccg cttcacgcta tgagtgagga tcaatcggca
     3001 attcaagccc tacggctgct gacgagtgcg gcggagttcg acagtgcaac aggcggtgtg
     3061 aatcgggaag ccaatcttta ccccgtcatc aagctgatta cccgagaggg cgtgcgcacg
     3121 ctgccggaaa cccagctgaa gcagaaattc gaggccagcg tttctcgatt tgcctaggag
     3181 tgtgaagcgg tcagcacggc ctttcgcgga aagtgaccgg tgggtgatcg ctggttatcc
     3241 tcgtcgcgcg gagagtgtac cgatgtacga agaaccgtat aagtgggtag aagcggttg
//
LOCUS       DASTQY010000445         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_263082, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000445 DASTQY010000000
VERSION     DASTQY010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263082"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VFR82_14295"
     CDS             <1..775
                     /locus_tag="VFR82_14295"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU4502819.1"
                     /translation="QVKQAEAALNAAELDLKYTTIRSPVNGIVVARNVEVGQTVAASF
                     ATPNLFLIALDLTKMQVDTNVSESDIGGITEGKEATFTVDAYPGIPFSGTIRQVRLAP
                     IAVQNVVTYNVVIGVENKDLRLKPGMTANVSIVVAQRDNVLKIPNAALRFSPQKFEPQ
                     ASRGTTVKSAPVPPLMAADGPSLRPIWKQTEDGDPVAVMIQTGISDGASTEILSGAVA
                     EDDEVLVGIEQPRGIRKSGDLPPGFGSSGGPRRSRDRGL"
     gene            833..1543
                     /locus_tag="VFR82_14300"
     CDS             833..1543
                     /locus_tag="VFR82_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4502820.1"
                     /translation="MHSFIQCDDIWKVYRLGDVDVQALGGLSLRIEKGEFVAVMGASG
                     SGKSTLMNILGCLDRPTKGHYRLEGRDVGELRADQLADIRNREIGFVFQSFNLIPRTS
                     ALENAQLPLLYRGLPLTEQRAKAAHVLQRVGLAGREHHYSTQLSGGQQQRVAIARALI
                     TNPSLLLADEPTGNLDTQSSREIMTMLEALNGEGITIILVTHEADIAAYARRQIVLKD
                     GHVLSDRITKARAQSVEV"
     gene            1544..>2160
                     /locus_tag="VFR82_14305"
     CDS             1544..>2160
                     /locus_tag="VFR82_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4502821.1"
                     /translation="MPAFIWLTVMTALRILARNRLRAGLTMLGIVIGVGAVIAMVSIG
                     EGAKRAVQAQIATMGTNVIIVWPGATTVSGVRGGQGGAVTLTVSDALDLKKKIPLLSE
                     TGWARREVMQMVNGNRNWNGPINGVSPSYLTIRDWAFSSGGPFTQADLDSAARVALIG
                     QTIVDNLFDEGEEPVGAAIRIRNVPFRVIGVLSPKGQSAQGSDQD"
BASE COUNT          466 a          611 c          665 g          418 t
ORIGIN      
        1 tcaggtcaag caagccgagg ccgccttgaa cgctgcggag ttggacctca aatatacgac
       61 catccgctcg cccgtcaacg gtattgtcgt cgccaggaac gtggaagtag ggcagaccgt
      121 cgcagccagt tttgcgacac ccaacctctt tttgatcgcc ctggatctca ccaaaatgca
      181 agtcgacacg aatgtcagcg aatcagacat tggcggaatc accgagggca aggaagcgac
      241 gttcacggtt gatgcctatc caggcatccc tttttcgggc accatccgcc aggtgcgatt
      301 ggcaccgatc gcagtccaaa atgtcgtgac ctacaatgtg gtgatcggcg tcgagaacaa
      361 agacctgcgc ctgaaacccg gcatgaccgc taacgtctca atcgtcgtgg ctcagcggga
      421 caacgtcctg aagattccga atgccgcgct gcggttctcg ccacagaaat tcgagccgca
      481 ggcgtctcgt gggaccaccg tcaagtcggc cccagtcccc cctctcatgg ccgcggacgg
      541 gccatcttta cgcccgatct ggaagcagac ggaagacggc gatcccgttg ccgtgatgat
      601 ccaaactggc atttcagacg gcgcctcgac cgagattctt tccggagcgg tggccgagga
      661 cgatgaggta cttgtcggaa ttgaacagcc acgtggcatc agaaagagcg gtgacttgcc
      721 tccaggattt gggtcgtctg gagggccgcg ccggtcgcgc gataggggtt tataagccgc
      781 tggtcgccta gtgcgaccga aacgcggcag cgctctccgc attccgttca ccatgcatag
      841 tttcattcaa tgtgacgaca tctggaaagt ctatcgcctt ggcgatgtgg atgtgcaagc
      901 gctcggtgga ctgagtctga gaattgaaaa aggtgagttc gttgccgtca tgggtgcatc
      961 cggttccggg aaatccacat tgatgaacat cctgggatgt ctggaccgtc cgacgaaggg
     1021 acattaccgg ctcgagggcc gagatgttgg cgaactgcgg gcggatcagt tggcggatat
     1081 cagaaaccgc gaaatcggat tcgtgtttca gagtttcaat ctgattccgc gcacgagcgc
     1141 gctcgagaat gcacaattgc ctctgttgta ccgagggctc ccgctcacgg aacagcgggc
     1201 gaaggccgcg cacgtgcttc agcgagtggg gctcgccggc agagagcatc actactccac
     1261 gcaactgtca ggcggtcagc agcaacgcgt cgccattgca cgggccctca tcacaaaccc
     1321 ttccctgctg ctggcggacg agccgaccgg caacctggat acccaatcga gccgggaaat
     1381 catgaccatg ttggaagcgc ttaatggcga gggcatcacg attatcctcg tcacgcacga
     1441 agcggacatc gccgcttacg cacggcgcca gattgtgttg aaagacggcc atgtcttaag
     1501 cgatcgaatc acgaaggcgc gtgcacaatc cgtcgaggta tagatgcccg cctttatctg
     1561 gctcaccgtc atgacggccc tgcgcattct ggcacgtaat cgcctgcgcg cagggttgac
     1621 catgttggga atcgtcatcg gagtcggcgc agtcatcgcc atggtgagca taggggaagg
     1681 agcgaagcgg gctgtgcagg cccaaatcgc cacgatgggg acgaatgtca tcatcgtttg
     1741 gcccggcgca acgacggtga gcggggtgcg cggcggccaa ggcggcgccg tgaccctcac
     1801 ggtctcggat gcgttggatc tcaagaaaaa gattccgttg ctgtcggaga cgggatgggc
     1861 aaggcgagaa gtcatgcaga tggtcaacgg gaaccggaac tggaatggtc cgattaacgg
     1921 cgtgtcgccg agctacctca cgattcggga ttgggcgttt tcgagcggag ggcctttcac
     1981 tcaggcggat ctcgacagcg ctgcccgcgt ggccttgatc ggtcaaacca tcgtggacaa
     2041 cctgtttgat gaaggcgaag aaccggtcgg cgcagcgatc cgaattagaa acgtcccgtt
     2101 ccgcgtcatt ggtgtcctct cgccgaaagg ccagtcggcg cagggatccg atcaggatga
//
LOCUS       DASTQY010000446         9384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_106043, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000446 DASTQY010000000
VERSION     DASTQY010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106043"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFR82_14310"
     CDS             <1..482
                     /locus_tag="VFR82_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4502822.1"
                     /translation="VIIMTTNLGTRDISKGVNLGFQSTDAEQDRHKKEEVLGELRRQF
                     SPEFLNRVDEIVVFHQLDKTHLTSILDILLRELNLRLVDKGVEIEVDDEVKVWLIKEG
                     YEPLYGARPMRRTIQRAIGDPLSEEMIKGRFKESRKIRVVLRDGAPTFIEQEAMAGV"
     gene            589..1671
                     /gene="tsaD"
                     /locus_tag="VFR82_14315"
     CDS             589..1671
                     /gene="tsaD"
                     /locus_tag="VFR82_14315"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU4502823.1"
                     /translation="MINRDLASPWQSGPVLGLETSCDETAASVLASDGSVLSNVIASQ
                     DAIHQRFGGVVPELASRAHVETIEFVAAAAMEEAKLTWADLAGLAVTQGPGLAGALIV
                     GVSYAKSLAYTLGLPLVGVSHLEGHIASAWLEDPEFPLPCVVLVVSGGHTHLFHQDRQ
                     GRSRLLGCTRDDAAGEAFDKGAQMLGLGFPGGPALDRLAQTGKAHAIVFPKSRINKDS
                     LEFSFSGLKTALLYKLHGMDERQQGKLKADLAAGYQEAIVTVLIEKAFMALKQTGLRA
                     LAVVGGVSANSRLRSLLQERALATDTRVSFPALRFCTDNAAMVASAGRQALIAGRRMP
                     LNAEPWATLEPTIETHFAITPEGAHS"
     gene            1694..3346
                     /gene="hflX"
                     /locus_tag="VFR82_14320"
     CDS             1694..3346
                     /gene="hflX"
                     /locus_tag="VFR82_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU4502824.1"
                     /translation="MRPSQITAIERLYRRRVPHDKVLSPELGRTMCQISREIRRPIAI
                     LLSRRGQVQEIIVGTDLTLSAATLSKFRVGARSLRGLRVIRTQFHDQPLSQESLTDLA
                     YLRLDLFGALSVTSEGNPGNLYLAHLLPPNGTGQVCNILKHTPFHHSIMAFDRFIEDL
                     ESDMQRARGTHAVGHERESAMLVSASSRSRVEQEDRLVELAELASSAGVTVVDRMAQR
                     TQDGHQRYLLGSGKLKDVLIQTLHRGADMVIFDQTLTPAQSRAISEMTDIKVIDRTQL
                     ILDIFARRAHSREGKVQVELAQLRYLLPRLSGKGQELSRLGGGIGSRGPGETKLETDR
                     RRIRDRITHLERELDQFGRHQDQRRARRTRHGLPVVSLVGYTNAGKSTLLNVLTNSHV
                     SADDRVFETLDTTSRRLRFPQDREVIITDTVGFIRDLPEELLGAFRTTLEELRQADLL
                     VHVVDASAVDVDIQISAVVKILQELNLEMLRRTLVFNKCDRIPQATADLLCHRYQAIG
                     VSALQPDTLPPLLDHLDRELAAVAAPQPSNQVDQAVALASRL"
     gene            3375..4037
                     /gene="nth"
                     /locus_tag="VFR82_14325"
     CDS             3375..4037
                     /gene="nth"
                     /locus_tag="VFR82_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU4502825.1"
                     /translation="MRRTPTSPAATRKRLQRIAQALQAAMPDAKVELSHQSPWELLVA
                     TILSAQCTDQRVNQVTPALFSRYKTPSDLAKARLSDVEAIIRSTGFFKSKAKNIVACG
                     QVLNERFNQRVPERMDDLVTLPGVGRKTANVLLGQAFGQPAIVVDTHVKRVANRLDLT
                     TSDDPVQIEQDLQQLLPSSQWTRVSQRLLLHGRYICLARRPLCGRCPIYDYCYATEKL
                     PR"
     gene            4046..4912
                     /locus_tag="VFR82_14330"
     CDS             4046..4912
                     /locus_tag="VFR82_14330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEU4502826.1"
                     /translation="MTGFGRKQAPWQEGTITVEVRSVNHRFLEVSCRLPRTLATLEEI
                     FKKAVQQRCTRGRVDLTVTIQGTKGRSGTVALDHALAKQYHHALRALKASLKLSGPID
                     VALIAGLRDVVSVSDQPPEEPELARMVQQLANKALDDLHDMRKQEGLALAKDMQKRVE
                     TMKDHRSAVASRSPIVVQETFERMKERIAKLLGTDPPDLPRLHQELAVYADRGDITEE
                     IVRLDSHMLQFEQQLNRAESVGKTLEFLLQEMGREVNTIGSKANDAAIAGHVIHMKAE
                     LERIREQVQNVE"
     gene            4933..5610
                     /gene="gmk"
                     /locus_tag="VFR82_14335"
     CDS             4933..5610
                     /gene="gmk"
                     /locus_tag="VFR82_14335"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU4502827.1"
                     /translation="MPSGPVRSSTERRGILFIISAPSGTGKTTLCKQLVASIPGLWHS
                     VSYTTRKPRPGEEHGREYYFTDDATFQAMIDRHEFVEWARVYGHLYGTPRRTLADKIE
                     QGIDVLLEIDVQGALQVKKKFDDAVSIFILPPSMTVLRQRLLTRASDSSEEIQRRLQK
                     VKEEVWSYREYAYIVRNDDLTRSLQDLESIFLGERLKTKRLNMAWIENNFILNDEAKP
                     GDRKDLP"
     gene            5628..5984
                     /locus_tag="VFR82_14340"
     CDS             5628..5984
                     /locus_tag="VFR82_14340"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU4502828.1"
                     /translation="MIDMLSLLPQYTPDQFDSRHRLVIVAAQRAKHIVQGSRPFGMSR
                     FTKETTIALDEVLRGEAKYLIGKDARDAMKEAKRGKEGETERMAMMTGEDAREIKKEL
                     SVYVDDSPKPAGEPEE"
     gene            5988..7238
                     /gene="coaBC"
                     /locus_tag="VFR82_14345"
     CDS             5988..7238
                     /gene="coaBC"
                     /locus_tag="VFR82_14345"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEU4502829.1"
                     /translation="MEGPARNPTLTGARIVLGVTGSIAAYKAVTLLRALAREGAVVDV
                     AMTRSATKFVTPLTFEVLSGRPVTADLFQAHQEMKHLSLPEQADVIVVAPATANFLAK
                     AALGMSDDLLSTMLLTGRCPLVCAPAMDGGMWFHPTVVAHVEALRARGAIIVDPEEGP
                     LASGRIGQGRMAGEERILDAVRAVLTPKRDYQGQRVLLSAGPTQEPIDPVRYLSNRSS
                     GKMGYAIAEAARKRGADVILVSGPTSLSPPPGVEVIPVETAEEMAKQLSTRLAWSTVV
                     VMAAAVADFRPKQVAPEKLKKQGRQSATLELEQTTDILSTLSGQRTTQVLIGFAAETH
                     DLLPHARAKLASKGLDLIVANKVTTPGAGFGSDFNAATLIDREGHTTDIPLKSKRELA
                     DDILDAVRRLLGDSAARAHLRKRR"
     gene            7241..7996
                     /locus_tag="VFR82_14350"
     CDS             7241..7996
                     /locus_tag="VFR82_14350"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4502830.1"
                     /translation="MPPQKNIASALEIDRLATQLATNPRSKAFLPLAEEYCKAGMWEE
                     AVGVLEDGLKYYPGFITAMVVLGRAYDQLNQPTKAKAVLEGAVKLSPENLRAHRTLLK
                     IYATQGLTEEALKSCRVILAMNPRDEEVLSLQASLGIQESEKLQGASHQKYAEVTGRE
                     SVQPASRTARGPAAALEDVPMSTVDIAAGKPSYPFTVQKPTQGIASSDPEAMTTASNQ
                     ASHADTVVQLESWLRSLERQRRDRPTGETHSGS"
     gene            8106..8600
                     /gene="aroQ"
                     /locus_tag="VFR82_14355"
     CDS             8106..8600
                     /gene="aroQ"
                     /locus_tag="VFR82_14355"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEU4502831.1"
                     /translation="MLRILVLHGPNLNLLGTREQSIYGTQSLENIDSALTTLAENLGL
                     HLDIRQSNSEGDLVNWIQESGSGYHGIIINPAAYTHTSIAIRDAISAVGLPTVEVHLS
                     NIHQREPFRHHSHVAGVALGQISGLGPSGYLLALRGLHDHLAGTRKLKNPPTASRRQK
                     RASR"
     gene            8640..9200
                     /gene="efp"
                     /locus_tag="VFR82_14360"
     CDS             8640..9200
                     /gene="efp"
                     /locus_tag="VFR82_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU4502832.1"
                     /translation="MISTTDFRGGVRLLVDGEPYYIVEFQHVKPGKGGAFVRTKLKSY
                     LSGNVLDRTFRSGERFEEPDLEEREMQFLYATGNEYTFMDTETYEQLTFEKKQLGDNA
                     DLLKENMNAKILIYEHRPIDVELPIFIELKVVDAEPGVRGDTASGGTKPAVVETGATI
                     KVPLYLEVGTVIKIDTRTRAYVERVR"
     gene            9197..>9384
                     /locus_tag="VFR82_14365"
     CDS             9197..>9384
                     /locus_tag="VFR82_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502833.1"
                     /translation="MSRKKPRKSTRHGIPIVLPQSFAGGAGAQDAAAQQRQIQDMIDL
                     LKRNHWTELEMERAGIRI"
BASE COUNT         2160 a         2691 c         2677 g         1856 t
ORIGIN      
        1 ccgtgatcat catgaccacc aacctcggca cccgtgacat ctccaagggc gtcaacctcg
       61 gattccaaag cacggatgcc gagcaggatc ggcacaagaa ggaagaggta ctgggagaac
      121 tccgtcggca attcagccca gagttcctca accgcgtcga cgagatcgtg gtcttccacc
      181 aactcgacaa gacgcacttg acgagcatcc tcgacatcct gttgcgcgaa ctgaatctga
      241 gattggtgga caagggcgtc gaaatcgaag tcgacgacga ggtgaaggtg tggctgatca
      301 aggaagggta tgagccgctc tatggagcac ggccaatgcg cagaacgatc cagcgcgcaa
      361 tcggcgatcc cctctctgag gagatgatca aaggccgatt caaggagagc cgcaagattc
      421 gcgtcgtatt acgtgatggt gcgccgacct tcatcgaaca ggaggccatg gcaggggtct
      481 agcaaccgcc gtgacaccac atcactcctt tgaccccctg tgcgaccctt gtcggaatga
      541 cggcaagggt cgcattgttt ttacggcagg ccgggtttgt ctccgatcat gatcaaccgc
      601 gatctcgcct ctccttggca atcaggtccc gtcctgggtc tcgaaacctc ttgtgacgaa
      661 actgccgcct cagtcctggc atccgatgga tcagtcctct ccaacgtcat tgcgtctcaa
      721 gatgccatcc atcaacgttt cggtggcgta gtcccagaac ttgcctctcg tgctcacgtc
      781 gagacaatag agttcgtcgc agccgcggcc atggaggaag cgaagctaac atgggcagac
      841 ctcgccggcc ttgcggtcac ccaaggacct ggccttgccg gtgcgctgat cgttggcgtc
      901 agttacgcca agagcttggc gtacacgctc gggcttcccc tcgttggcgt cagccatttg
      961 gaagggcata ttgcttccgc atggctggag gatccggagt tcccgttgcc ttgtgtcgtg
     1021 cttgtcgtgt cgggcggcca tacgcacctc tttcatcaag atcggcaagg ccggagtcgt
     1081 ctgctgggct gcacaagaga cgatgccgcg ggagaagcat ttgataaggg tgcgcagatg
     1141 ctgggattgg gattccccgg cggccccgca cttgatcggc ttgcgcaaac aggcaaggcc
     1201 cacgcgatag tgtttccgaa gtcccgcatc aacaaagaca gcctcgaatt cagttttagc
     1261 ggattaaaaa ccgcgttgct gtacaagctc catggcatgg acgaacgaca gcaaggcaag
     1321 ctgaaggccg atttggccgc aggttaccaa gaggctatcg tcaccgttct tatcgaaaag
     1381 gctttcatgg ctctcaagca gacgggcctg cgcgccctgg cggtggtggg tggagtgtcg
     1441 gccaatagcc gcctgagatc cctgcttcaa gaacgagcct tggcgaccga cacgagagtc
     1501 agctttccgg ccttacgctt ttgtacggac aacgccgcaa tggtggcgtc agcaggcaga
     1561 caagccctta tcgccggtcg gcggatgccg ttgaatgcgg aaccatgggc cacgttggaa
     1621 ccgacaatcg agactcattt cgctataacc cctgagggcg cccattcctg agatctacgg
     1681 tcagaccagc ggattgcgac ccagtcaaat caccgccatc gagcggctgt accggcgtcg
     1741 cgtgcctcac gacaaggtgc tgtctcctga gctcggcagg accatgtgtc agatctcacg
     1801 cgagattcgg cgtccgattg cgatcctgct cagtagaagg ggacaggttc aagaaatcat
     1861 cgtcggaact gatctcacgc tctccgcggc gaccctgtcg aagtttcgtg tcggagcccg
     1921 gtctctgcgc ggccttcgtg tcatccggac acagttccat gatcagccgt tgagccagga
     1981 atctttgacc gacctcgcct atctccgtct tgatttattc ggtgcgttgt cagtcacgtc
     2041 tgaagggaat cccggcaacc tgtatctggc gcatctcttg ccgccaaacg gtacgggtca
     2101 ggtatgcaat attctcaagc acacgccctt tcatcactcc atcatggcct tcgaccgctt
     2161 tattgaggat ctcgaatcag acatgcaacg tgcgcgcgga acccacgcgg taggccatga
     2221 gagggaatcg gccatgctcg tgagcgcctc atcacgaagc cgagttgaac aagaagatcg
     2281 cctggtggaa ctggcagagt tggcgtcttc tgcgggtgtc acggtcgtcg accgaatggc
     2341 gcaacgaaca caggacggtc atcaacgtta cttgttgggg agcggcaagc tcaaagatgt
     2401 cctgatccag acgctccaca gaggggcgga catggtcatc ttcgatcaga ctctcacacc
     2461 cgcgcaatcc agggcgatat ctgaaatgac ggacattaag gtcattgatc gaacccagct
     2521 catcttggac atcttcgctc ggcgggctca cagccgtgag ggaaaagtac aagtcgagct
     2581 tgcgcaactc cgatatttgc ttccaagact ttccgggaaa ggccaggagc tgtcccggct
     2641 cggcggagga ataggctcac gcggccccgg cgaaacgaag ctggaaacgg atcggcggag
     2701 aatcagggac cgcatcacgc atctcgaacg cgaactagat cagttcggcc gtcaccagga
     2761 tcagcggcgc gcccgtcgaa cgaggcacgg gctgccggtg gtttcgctgg ttggatacac
     2821 caatgccgga aaatcgaccc tcttgaatgt ccttaccaac agtcatgtct ccgctgatga
     2881 tcgggtattt gaaacacttg atacgacaag ccgcagactt cgattcccgc aagatcgcga
     2941 ggtgatcatc acggacaccg tcggattcat ccgtgattta cctgaagaac tgctgggagc
     3001 ctttcgcacg accctcgagg aattacgcca agcggatctt ctggtccatg ttgtggacgc
     3061 cagcgcggtg gatgtcgaca tccaaatatc cgccgtggtt aaaatccttc aagaactgaa
     3121 tctggagatg ctgcggagga cgctcgtctt caataaatgc gatagaattc cgcaggccac
     3181 tgcagacctg ctttgtcatc gctatcaggc catcggcgta tccgcgcttc agcccgacac
     3241 tttgccgccg ctcctggacc atctcgaccg cgaactcgcg gcggtggcgg cccctcagcc
     3301 atcgaaccag gtcgaccaag cggttgcgct tgcatctcga ttgtgacctc ttcacaatct
     3361 gcagcagcca gaacatgcga cgtacgccaa catcgccggc ggcgacccgg aagcgattgc
     3421 aacgcattgc gcaggcactt caggctgcga tgccggacgc caaggtcgaa ctctcccatc
     3481 aatccccctg ggaactgttg gttgccacca tcctgtcggc gcagtgcacc gatcaacggg
     3541 tcaaccaagt cacaccggcg ctgttcagcc gatacaagac gccctccgat ctggccaagg
     3601 ctcgcctatc agatgtggaa gcgatcatcc ggtcaaccgg attcttcaag agcaaagcga
     3661 agaacatcgt ggcctgtggc caagtcctca atgaacgatt caaccaacgg gtaccggaac
     3721 gaatggatga tttggtcacc cttcccggag ttggacgcaa aacagccaat gtcctgctgg
     3781 gtcaagcctt cggtcaaccg gcaatagtcg tagatacaca cgtgaagcgc gttgccaatc
     3841 ggctcgatct gaccacatcg gacgatccgg tgcagatcga acaggatctc cagcaactgc
     3901 tgccgtcatc gcaatggacc agagtgtctc agcgtctgtt gttgcacggc cgctatatct
     3961 gtcttgcccg gagacccttg tgcgggcggt gcccgattta tgactactgc tatgcgacgg
     4021 agaagctgcc tcgatgattc atagcatgac cggatttggg cgaaaacaag cgccgtggca
     4081 ggaggggacc attaccgtcg aagtgcggtc tgtgaaccat cgatttctcg aggtgtcctg
     4141 cagattgccc cgtaccttgg ccacgctcga ggagattttt aagaaagcgg ttcagcagcg
     4201 ctgcacgcgt gggcgtgtcg atttgacggt cacaattcaa gggactaagg gacggagcgg
     4261 gaccgttgcc cttgaccatg cgctggcaaa acagtaccat cacgccctcc gtgcgctaaa
     4321 agcctctctc aaactgagcg ggcctattga tgtggccttg atcgcgggac tccgtgatgt
     4381 cgtgtcggtc tcagaccaac ctcccgaaga gcctgaattg gccagaatgg ttcaacaact
     4441 ggccaataag gcgttggacg accttcatga catgcgaaag caggaaggcc ttgccctggc
     4501 caaggacatg cagaaacgcg ttgagacgat gaaggatcac cggtctgccg tggcgagtcg
     4561 ctcgcccatc gtggtgcagg aaaccttcga aaggatgaaa gaacgcatcg caaaactact
     4621 gggaacagac cctcctgatc ttccgcgact gcaccaggaa ttggccgtct atgccgaccg
     4681 tggcgacatt acagaagaaa tagtcagact ggactcccat atgctacagt ttgaacagca
     4741 gcttaaccgt gcggaatctg taggtaaaac gctcgagttc ttgctccagg aaatgggccg
     4801 ggaagtcaat acaatcggct cgaaggcgaa cgatgccgct atcgcggggc atgtgattca
     4861 tatgaaagcc gaactggaac gcatccgaga acaggttcaa aacgtcgaat gaacaggtcc
     4921 agcaccactc cgatgccttc cgggccggtc cgatcttcca cggaacggcg ggggattctc
     4981 tttattattt ccgcaccgtc cggtaccggg aaaaccacat tgtgcaagca gctcgtggcg
     5041 agcattcctg gactctggca ctcggtgtcc tacacgacga ggaagcctcg tcccggcgaa
     5101 gaacacggtc gcgagtatta ttttaccgac gatgccacct tccaagcgat gatcgaccga
     5161 catgagtttg tcgagtgggc gcgcgtgtac ggccacctgt atggaactcc gagaaggacg
     5221 ttggccgaca agatcgagca aggcatcgac gtgttgttgg agatcgacgt gcagggggct
     5281 ttgcaagtga aaaagaagtt cgacgacgcc gtgtcgatct tcatccttcc gccttcgatg
     5341 accgtgctcc gtcagcgcct actgactcgc gcctccgatt cctctgagga aattcagcgg
     5401 cgactgcaaa aagtaaaaga ggaagtctgg agctatcggg agtatgctta tattgttcgc
     5461 aacgatgatc tcacgcgttc gttgcaagat ctcgaaagta tttttctggg agaacggttg
     5521 aaaacaaagc ggctcaatat ggcctggatc gaaaacaact tcattctaaa cgacgaggcc
     5581 aaacccggag atcgtaaaga tcttccctaa cccaaggagt cagatccatg atcgacatgc
     5641 tcagcctgtt accgcaatac acgccggacc agtttgactc ccggcaccgc ctcgtcattg
     5701 ttgctgccca gcgcgcgaaa cacattgtgc agggatcgcg tccctttggg atgtcgcgct
     5761 tcacgaagga gacaaccatt gcgctggacg aagtgctgcg cggggaagcg aaatatctca
     5821 tcgggaagga tgcgcgagac gcgatgaagg aagccaagcg cggcaaagaa ggcgagacgg
     5881 agcgcatggc catgatgacc ggagaagacg caagggaaat caaaaaagaa ctcagcgtct
     5941 acgtggacga cagccccaag ccggcagggg agccagaaga atagcgcatg gaaggccctg
     6001 ccagaaaccc tacgctcacc ggtgcacgca ttgtgctcgg cgtgacgggg agtatcgccg
     6061 cctacaaggc agtgactctg ctgcgtgcgc tcgcgcgaga aggggcggtg gtcgacgtag
     6121 ctatgacccg ctctgccacg aagttcgtga ctcccttgac ctttgaagtg ttgtctggac
     6181 ggccggtcac cgcagacctg ttccaagcgc atcaggaaat gaagcatctc tcactgcctg
     6241 agcaggccga cgtcattgtg gttgcacccg cgaccgcaaa tttcctggca aaggcggctc
     6301 ttggaatgag cgatgacttg ctgtcaacca tgctcttgac ggggcgatgt ccgcttgtct
     6361 gtgctcccgc aatggatggc ggtatgtggt tccaccccac ggtcgtcgcc catgtggagg
     6421 cgctacgggc tcgtggcgcg atcatcgttg atccggaaga aggcccgctc gcctccggtc
     6481 ggatcggtca aggacgaatg gctggagagg aacgtatttt ggacgccgtg cgtgctgtac
     6541 tgactccgaa gcgggattat cagggtcaac gggtcttgct gtcggctggc ccgacgcaag
     6601 agccaatcga tccggtgcgc tacctctcga accggtcgtc gggcaagatg ggttatgcca
     6661 tcgccgaagc agcacggaag cgaggcgctg acgtcattct ggtcagcggc cccacttcgc
     6721 tttctccgcc cccgggagtc gaggtgatcc cggtagagac agccgaagaa atggcgaagc
     6781 agctgagcac cagattagcc tggtctacgg tcgtcgtcat ggccgccgcc gtggcagatt
     6841 ttcgtcccaa gcaggtcgct cctgagaaat tgaaaaagca gggtagacaa tccgctacgc
     6901 ttgaactgga gcagaccacc gatattcttt cgacgctttc aggccagcgc accactcaag
     6961 tgctgatcgg ctttgcggcg gaaacccatg atctcctgcc ccacgcccga gcgaaactcg
     7021 cgtcgaaagg cctggacttg atcgtcgcga ataaggtgac cacgccagga gccgggtttg
     7081 gcagcgactt caatgccgcc acgctaatcg accgagaggg tcacacgaca gatattccgc
     7141 tgaagtccaa acgtgaattg gcggacgaca ttttagatgc cgtacgccgg ttgctcgggg
     7201 actctgctgc ccgggcacac ctccgcaaaa ggcgctaacg atgccccctc agaaaaatat
     7261 cgcgagcgcg ttggaaatcg atcgactcgc cacgcaactg gcgacaaatc cacgttcgaa
     7321 ggcatttctt ccgctagcgg aagaatattg caaggcggga atgtgggagg aagccgtcgg
     7381 cgtactggaa gacggtctga agtattaccc cggattcatc acggccatgg tggtgctggg
     7441 acgagcgtat gaccaactga atcagccgac caaggcgaaa gcagtcctcg agggcgccgt
     7501 caagctcagt ccggaaaatc tccgcgcgca tcggactttg ctcaagatct atgccacgca
     7561 aggactcacc gaagaagccc tcaaatcctg tcgcgtgatc ctcgcaatga acccacgaga
     7621 cgaggaggta ctttccctcc aagcatcatt gggcatccaa gaatccgaga aactacaagg
     7681 cgcctcacac cagaaatacg cggaagtcac aggccgagaa tctgtacaac ctgcctcaag
     7741 aaccgcgagg gggccagctg ccgcgcttga ggatgtcccg atgtccactg ttgacattgc
     7801 cgccggaaag ccgagttatc cgttcaccgt tcagaaaccg actcagggta tcgcctcttc
     7861 tgatcctgag gcgatgacca ctgcgtcaaa tcaggcaagt catgctgata ccgtcgtaca
     7921 gctcgagtcg tggttacgct cactcgagcg gcagcgccgg gatcgaccga caggagagac
     7981 gcactccggc tcataagctc ttaatatctt tcgacagttt gaccgctctt cagtagcctg
     8041 ttacaatgcc gctctcctaa cgctgccggc acgaacacca ccaggttttg aatacatcgg
     8101 ctatcatgct ccggatactc gtcttacatg gacccaactt gaatttgctc ggtacccggg
     8161 aacagtccat ctatggcacc caatcgctcg agaatatcga ctcggcgcta acaacgctcg
     8221 ccgagaattt agggttgcac ctcgacattc gccaatcgaa cagcgagggc gacttggtga
     8281 actggattca ggagagtggg agcggctatc acggcatcat catcaatccg gccgcctaca
     8341 cccatacgag cattgcgatc cgcgatgcga tttccgctgt gggcctgccc accgtcgaag
     8401 tccacttgtc gaacatccat cagcgcgaac catttcgaca tcattcccat gtggcgggag
     8461 tggcattagg ccagatatcc ggtttgggac cgtcgggcta tctcctcgcg ctgcgaggcc
     8521 tccacgatca tttagcaggg accagaaagc tcaagaatcc tccgacggcg tcccgccgac
     8581 aaaaacgggc gtcacgatag ggcagcggtg aacctcatag acggtgaagg gagtaacgag
     8641 tgatttccac gactgatttt cgcggcggtg tgcgcctgtt agttgacggc gagccgtatt
     8701 acatcgtcga atttcaacat gtgaaaccag gaaagggcgg cgcgttcgtc cgaaccaagc
     8761 tgaagagcta tctctccggc aatgtgctgg accgcacctt ccgatcgggt gagcgctttg
     8821 aagagccgga cctggaagaa cgggaaatgc agttcttgta tgccaccggc aacgaataca
     8881 cgttcatgga caccgagacc tatgagcagt tgacgtttga gaaaaagcag ctcggagaca
     8941 atgccgatct cctcaaagag aacatgaacg cgaaaattct tatttacgag catcgtccga
     9001 tcgacgtcga gctgccgatc ttcatcgaac tcaaggtcgt ggatgcggaa ccaggggtgc
     9061 gcggcgatac ggcgtccggc ggtaccaagc ccgcggtcgt cgagaccggc gcgaccatca
     9121 aagtgccgct gtatttggaa gtcggcaccg tcatcaagat cgatacccgt acgcgggcct
     9181 acgtggagcg ggtccggtga gccggaagaa acctcgcaaa tcaacacggc atggaattcc
     9241 gatcgtgctg cctcagtcct ttgccggcgg ggcgggcgcc caggatgccg ctgcgcagca
     9301 acggcaaatt caagacatga tcgacctgct gaaacggaac cactggacgg agctggagat
     9361 ggaacgggcc ggcatacgaa ttag
//
LOCUS       DASTQY010000447         9963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_42575, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000447 DASTQY010000000
VERSION     DASTQY010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9963
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42575"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2214)
                     /locus_tag="VFR82_14370"
     CDS             complement(<1..2214)
                     /locus_tag="VFR82_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4502834.1"
                     /translation="MPFEAARSSSAGRELGIMWAQRHRSVKRFPIVVIGLVLLWASGE
                     GIAMAQAAPPADTGGARERELRDQLKNILQELEELQQQKDRARPEEERPSIIKEPAEP
                     VSPEVSARPDIDLADMSIVSQRLQKRPEGISLSATVPAETDSQPTRTMKESLESLPGV
                     VLRQANGPRDFSIMIRGQGAKTTFAVRDIKIYEDGFIQTQSDGLSRLDIHDPWFMRST
                     EVLRGASSSLYDNYALGGMVHFRTRRGSDINGFETFLSGGSYGFFKQAFAVGQQFQNL
                     DVSLFASNAAEDGFIRNSDYNTQTLNFNLRFSIDDKQSFYFKAITNWLDTRVPTRLTQ
                     SQFLADERQAGGSQTACTPGIYNPNCANASLLHQQRIDRRTIVGGIYERQLNANTVLT
                     IEADYDVKDINQYFSQITDNVNPNYKNYVDLRHDGRLAEMPLRSYVGFFVNNMEQEGQ
                     TFQNLADGNGTRGLLIQNNRGTIRNIGGRFREELEFVPKWILAGGLGFEQSMISVQTI
                     NYEGATPLRVNADRTYYNWAPEMSLTWKPADGYRHWVRASTGYGIPQFTNLLRDPVTG
                     LPGTNFDLKPQKNLNTEIGTESRLTKDLVVELVGFWTFFKNEIITQTISGANTASVNA
                     DSSQYRGIEARYDWRPLTGLRFSGAYTHIDAKYINFSDRTAAGFLTRDGNKVPNVPTD
                     IFNTKVEYDHTPSGWGGWIEGSYYNSYFLNNSNTFGIPSYIVMNVNVHKSIEIKNS"
     gene            complement(2579..3148)
                     /locus_tag="VFR82_14375"
     CDS             complement(2579..3148)
                     /locus_tag="VFR82_14375"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU4502835.1"
                     /translation="MGHEKYRSFLAALIAAYAVMFGFDGSLSGADPSADPFVALRVSR
                     VPTGNPIDTFDLKALDGGTLRSADLIGKVVLLNFWATWCGPCKEEMPSLGRLQEQFDS
                     ARVRVVTVTADIYPQGIRQFLDQLDIRLPVAFDEDQELSRKLSVRGLPTTVVIGQDGH
                     IVGRAVGPRQWDSSESIALVRHVLAADRP"
     gene            complement(3138..4457)
                     /locus_tag="VFR82_14380"
     CDS             complement(3138..4457)
                     /locus_tag="VFR82_14380"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU4502836.1"
                     /translation="MKKGSARTHRYRVSIHSSLILFIVGLIALWPIVSAVAGTQHPPV
                     SFGAKHMSVHHSKTVVGPSVQIDEQGIIFAAWVEEDKDTRTILFARSEMPGGPLGTPV
                     PVNRPSESPYYRQESPAVAVRGGEIFVTWSKTHPRIAPDKPFSGELRLSRSIDGGRSF
                     APSTLVNDDEQVIQHAFDAVQIAPDGAVHLAWIDGREGKKEPGTFVARSTDHGRTITK
                     NFKVDDNTCVCCRTAVAASADAVYVAWRKIFDGNVRETVVATSTDGGETFSSPVVVGH
                     DQWVYQACPHRPASMGVDRAGRLYVVWYTEGADETPAIYLAYSDDRGNTFSDKKQLNR
                     SKGTFPDHPQLAVDPEGRVAVIWEEQSPVRREIVMSHSVDRGQSFSVPFKLNEKNGQT
                     PTIAVNQHGTVVMGWKEHAMPAHRLIVQTMQLPAVAVAEAEIVNHGP"
     gene            complement(4462..5499)
                     /locus_tag="VFR82_14385"
     CDS             complement(4462..5499)
                     /locus_tag="VFR82_14385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU4502837.1"
                     /translation="MTSTDQNRFLLPAWGFSLLLHGLAVLLAVIFAAQVKPVLKEEVF
                     KWDVALVDAVRTESLPEPAQPIAPEVQTHSKPVVPRPSTSTRETALNRVAPHQSVEMV
                     HSRPQPAKPIEQKADLIAPPQFEPVEQMAEVRPQAHEPIEQQAVEVPKSDPQPVLEAE
                     KPEPAPHVEPVIAQRQPLAPIPPAETKEDRVAQPPVSETSTVEPVREANIQNPAQPAT
                     SAEPTTPSPPKASLPEASTQVAKTAPGPDTKIDHRWVGESLWRRVGELKRYPTSARLN
                     GQEGKVILKAVIRADGHLAEVSVQKSSGHHILDTAAMEAVRLACPLHMKHAISKSEIV
                     VSLPIVYSLAN"
     gene            complement(5583..5891)
                     /locus_tag="VFR82_14390"
     CDS             complement(5583..5891)
                     /locus_tag="VFR82_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502838.1"
                     /translation="MSWSPKKSLAVVLVLCLLVIGGLGSAQAISHESHHAHHQKSTHA
                     TVLCAWMCAAGQVLDTVTAPDLIERSPVSRVECSALPDFSSGFSSLLSSRGPPTDVTI
                     "
     gene            6063..8777
                     /gene="secA"
                     /locus_tag="VFR82_14395"
     CDS             6063..8777
                     /gene="secA"
                     /locus_tag="VFR82_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU4502839.1"
                     /translation="MLTQILNFIFGSKNDREIKGLMPVVHRINGLESDLIPLSDQALA
                     DKTQTFRKRLAAGETLDDILPEAFAVCREMSRRKLNMRHFDVQLIGGMILHKGRISEM
                     KTGEGKTLVATLPIYLNALEGKGAHLVTVNDYLAKRDAQWMGQLYHALALSTGVIQHD
                     ASFIYDPTYDASDKRLQHLRPCTRYEAYRADITYGTNNEYGFDYLRDNLVVTALDQCV
                     QRDLNFAIVDEVDSILIDEARTPLIISGPTDQSTDLYYRINAIIPQLKPEHDYTIEEK
                     TKTAALTEEGNVRVEKLLGVDNLYDPANMDLVHHVVKALQAYALYKRDVDYVVKDGEV
                     IIVDEFTGRLMPGRRWSDGLHQAVEAKEGVKIANENQTLASVTFQNYFRMYKKLGGMT
                     GTADTEAAEFAKIYNLDVNVVPTNRKMIRLDYADVVYRTEKEKFAAIVEEIKDCHQRG
                     QPVLVGTISIEKSEKIAGMLSRNGIKHNVLNAKQHEREAEIVAQAGRKGAVTIATNMA
                     GRGTDILLGGNADFMFKQVLYREEQLAEERKLAVYEEIRADCEQNKQEVVSLGGLHIL
                     GTERHESRRIDNQLRGRAGRQGDPGSSRFYLSLEDDLMRIFASERVSQLMLKLGMEEG
                     IPIEHGMVTRAIANAQKKVEAHNFEIRKQLLEYDDVMNKQREVIYQHRRAVLAGENLS
                     DDLREMMENLVESALNVYCPAEQYPEEWDMKGLAEMMQGQFGLDITQGKQDGGESLRD
                     VGRDALLEDLKAQIRDGYDRKEKELGPELMRFLEKTFMLQVIDHHWKDHLLGMDHLRD
                     GIGLRGYGQKDPLIEYKREGYDLFAGMMERIKSDTIERLFLVQAVREGERSAPPPSVL
                     SRPQPKLTLNRGEEPVSTPTVHRSDDKVGRNDPCPCGSGKKYKKCHGA"
     gene            9343..>9963
                     /locus_tag="VFR82_14400"
     CDS             9343..>9963
                     /locus_tag="VFR82_14400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU4502840.1"
                     /translation="MSTGHPQLVAAIASEIAREGPIPFVRFMELALYHPQFGYYMRFP
                     EGGAERIGWNGDFYTSSDVHPILGQAIARQAAQIDALLGYPNPFTVVEMGPGKGLLAS
                     HVLSGLQLAPQSLAHRLRYVLIECSPAMRAMQQRHLDSWLRQTGCVSWVEDVNSLARE
                     SVRGLMFSNELPDAFPVHRVQQKDHALWEVFVDVRDGGFVECLKSLS"
BASE COUNT         2210 a         2839 c         2822 g         2092 t
ORIGIN      
        1 ggagttttta atttcgatgc tcttgtggac gttcacgttc atgacgatat acgacgggat
       61 gccaaaggtg ttgctgttgt tcaagaaata gctgttgtaa tagctgccct caatccagcc
      121 tccccaaccg ctaggcgtat gatcgtactc aactttggtg ttgaagatat cagtcggcac
      181 gttaggcacc ttgtttccat cgcgggtcaa gaagccagcc gcagtgcgat ccgagaaatt
      241 gatatacttc gcatcgatat gcgtataggc ccctgagaaa cgcaatcccg tgagcgggcg
      301 ccaatcatat cgagcctcga ttccgcgata ttgcgaggag tcggcattga cggatgcggt
      361 attcgctccc gaaatcgttt gtgtaatgat ctcattctta aagaaggtcc aaaacccaac
      421 cagttccacg accaaatcct tcgtcagcct cgactctgtg ccgatttccg tattcagatt
      481 tttttgcggc ttgagatcaa aattggttcc tggaagaccg gtaacggggt ctcgcagcaa
      541 gttagtgaat tgcggtatcc cgtaccccgt cgaagctcgt acccaatggc ggtaaccgtc
      601 agccggcttc caagtcaagg acatctcagg cgcccagttg tagtaggtgc gatccgcgtt
      661 gacccgcaac ggcgtagccc cctcgtagtt aatagtctgg acgctgatca tggattgctc
      721 gaacccgagc ccacccgcga ggatccattt cggaacaaat tcgagttcct ctctgaaacg
      781 tccaccgata ttgcggatcg tgcctcggtt gttttgaatc agtaagccgc gagtgccgtt
      841 gccgtccgca agattttgaa acgtttgccc ttcctgctcc atgttgttca cgaaaaaccc
      901 gacatagctc ctcagaggca tctccgcgag ccggccatca tgcctcaaat ccacatagtt
      961 cttatagttt ggattcacat tgtccgtgat ctgggagaag tattgattga tatctttcac
     1021 gtcgtagtcg gcttcaattg taagcacggt attggcgttg agttgccgct cgtagatgcc
     1081 gccgacgatc gtgcggcggt cgatgcgctg ctgatggagc aacgaggcat tggcgcagtt
     1141 gggattgtag atgcccggtg tgcacgccgt ctgggagccg ccggcctggc gctcgtcagc
     1201 aaggaactgt gattgagtta atctggtcgg cacgcgcgta tccagccagt tcgtgattgc
     1261 tttgaaataa aaactttgct tatcgtcgat gctgaaacgc agattgaagt tgagcgtctg
     1321 ggtgttgtaa tcgctgtttc gaatgaaccc gtcttccgcg gcgttactag caaacaggga
     1381 cacatcaaga ttttgaaatt gctggcctac ggcaaaggcc tgcttgaaga agccgtagga
     1441 gcctccggag aggaaggttt caaacccgtt gatgtcactg ccgcgcctgg ttctgaagtg
     1501 gaccatgccg ccgagggcat agttgtcgta cagcgaagac gacgctccgc gcagcacttc
     1561 cgtgctccgc ataaaccagg gatcgtggat gtcgagccgg gagagtccgt cggattgggt
     1621 ctggatgaat ccgtcttcgt agattttgat atcgcgaacc gcgaacgtgg ttttggcgcc
     1681 ctgcccccgg atcatgatgc tgaagtcccg cggtccgttg gcttgacgca acactacccc
     1741 gggaagggac tccagcgatt ccttcatcgt ccgggttggc tgcgaatctg tttctgccgg
     1801 aaccgtggca gataaggaaa tcccttcagg tcgtttctgt agccgttggc tgacgatgct
     1861 catatcggcc aggtcgatat caggccgtgc cgacacttca ggcgaaacag gctcggctgg
     1921 ttccttgatg atcgaaggac gctcttcctc cggtcgagcc ctgtcctttt gctgctgcaa
     1981 ttcttccaat tcctgcaaga tattcttgag ctgatcacgt agttcgcgct cgcgtgcccc
     2041 acccgtgtcg gccggaggtg ccgcctgcgc catggcaatg ccctccccgg aagcccacaa
     2101 caacacaagt ccgatcacta cgatgggaaa tcgcttcaca gatcgatgcc gctgtgccca
     2161 catgatcccc agctcccttc cagcagatga cgaacgcgca gcctcgaacg gcatgatgaa
     2221 gatcctccgt tcgcggcaca atcgatgagt tgaatgagag atgtccgtga gacaggtcaa
     2281 cgagggggcg cgcgagagag ggacccggag tcaaacaacg tggcgcgggg ggttgaatcg
     2341 gctgatcgca ccatcgctat aagaacggag acggcaacaa ccggcacagc ggcggcaacc
     2401 gcaacgctcg gactaacttg gcacgcccac gcacagaggg gtgaatgcgc tacatgtgag
     2461 tgactatggt gatgggcggt cggcaggtcc gcatgaagga cgagacaatt ggcggcgcca
     2521 attgcaaggg ctgcgtacag cacgaccatt ccgatcgcga gagcagaaaa acagcgcttc
     2581 atggtctgtc cgcggccagc acgtgtcgca ccaaggcaat tgattcggaa ctgtcccact
     2641 gacgagggcc gacggcgcga cccacgatgt ggccatcctg cccgatcaca actgtggtcg
     2701 gaagtccccg tacagagagc ttgcgcgaca gttcttgatc ttcgtcgaac gcgacaggca
     2761 gacggatatc aagttgatca agaaattgtc tgatgccttg cggatatatg tccgccgtga
     2821 ctgtcacgac gcggacacga gccgaatcaa attgctcctg cagtctaccg agcgatggca
     2881 tttcctcttt acatggcccg caccaggtgg cccaaaaatt caagagtacg accttcccta
     2941 tgagatccgc cgagcgaagc gttcccccat cgagcgcctt cagatcgaac gtgtcgattg
     3001 gattgcctgt cgggactcga ctcacacgga gcgcgacgaa cgggtcggcc gatggatcgg
     3061 cgccagacag agaaccgtcg aaaccgaaca tcaccgcata tgcagcgatg agcgctgcga
     3121 gaaacgatcg gtatttctca tggcccatga ttgacgatct ccgcttccgc tacagcaaca
     3181 gccggtaact gcatcgtctg gactatgaga cgatgcgccg gcatcgcgtg ttccttccaa
     3241 cccattacga cggtgccgtg ttggttgacg gcaatggtcg gtgtctgtcc attcttctcg
     3301 ttcagcttga atggaacgct aaatgattgc cctcggtcga cagagtgact catcacaatc
     3361 tcacggcgta caggggattg ctcttcccaa attacggcca cacgtccctc cggatcgact
     3421 gctaattgcg gatggtcggg aaacgtccct ttcgagcggt tgagctgctt cttgtctgaa
     3481 aaggtgttcc cccggtcatc cgagtaggcc agataaatgg cgggcgtttc atccgcccct
     3541 tccgtgtacc acacgacgta gagccgtccc gcccggtcca cacccatcga cgccgggcga
     3601 tgcggacagg cctgatacac ccattgatcg tggcccacca cgacaggaga cgaaaaagtc
     3661 tcgcccccat cggttgatgt agcgaccact gtctcgcgga cgttgccgtc gaaaattttg
     3721 cgccaggcga catagacggc gtcggccgaa gcggcaaccg ctgtgcggca acagacgcaa
     3781 gtattgtcgt cgactttgaa attcttggtg atcgtgcgtc cgtgatccgt cgacctcgcg
     3841 acgaacgttc caggctcctt cttgccttcc cggccgtcga tccaggccag atgcaccgcc
     3901 ccatcagggg cgatttggac cgcatcaaac gcgtgttgaa tcacctgttc atcgtcgttg
     3961 accaaagtgg agggggcaaa agagcgtccg ccgtcgatgg accggcttaa tcttaattct
     4021 ccggaaaatg gtttgtcggg ggcgatcctg ggatgggtct ttgaccaggt gacgaagatc
     4081 tcgcctccgc gaaccgccac ggcgggcgat tcctgacgat agtagggact ctcgcttggc
     4141 cgattcaccg gaaccggagt tccaagcgga ccaccgggca tttccgatct cgcgaataga
     4201 atcgtacggg tgtctttgtc ttcctccacc caggcggcaa agatgattcc ttgctcgtcg
     4261 atctgtaccg agggacccac caccgtcttc gaatggtgga ctgacatatg tttcgcgccg
     4321 aagctcaccg gtggatgttg cgtcccggcc acggctgaga cgatcggcca caaggcgatc
     4381 agccccacga tgaacaggat gagtgacgag tggatagaga cgcggtaccg gtgggtccta
     4441 gctgatccct ttttcacggt attagttcgc caaactatag acgatgggaa gactgacgac
     4501 gatttccgat ttgctgatgg catgtttcat atgtaagggg caggcaagtc gaaccgcctc
     4561 catcgcagcc gtatcaagta tgtgatggcc ggaactcttt tgaacggata cctccgccag
     4621 atgtccatcc gcccgtatga ccgctttcaa gatcaccttt ccttcttgac cgttcaaacg
     4681 agcagacgtg ggatagcgtt tgagttcacc cacacgccgc cacagggact cgcccaccca
     4741 ccgatgatcg atcttggtgt cagggccggg agccgtcttg gccacctgag tcgacgcctc
     4801 tggcaggctg gctttcggcg gcgagggtgt ggttggttcg gcggatgtcg caggctgggc
     4861 agggttctgg atgttcgcct ctcgcaccgg ctcgacggtc gaagtctcac tgaccggcgg
     4921 ttgggcgacg cgatcctctt tcgtttccgc cggaggaatg ggcgcaaggg gctggcgctg
     4981 cgcgatgacc ggctcgacat gcggagccgg ctctggcttc tcagcttcaa gcacaggttg
     5041 ggggtcggac ttcgggacct ccacagcctg ctgttcaatc ggctcgtgtg cctgggggcg
     5101 cacctcggcc atctgttcaa ccggctcgaa ttgtgggggc gcaatgagat cggctttctg
     5161 ttcgatcggt ttggccggct ggggcctgga atggaccatt tctaccgact ggtgcggtgc
     5221 aacccggttc aaggccgttt cccgtgtcga tgtagacggg cgagggacga ccggtttgct
     5281 gtgagtctga acttcaggag ctatcggctg cgcgggttca ggcaaggatt cagtccttac
     5341 tgcgtcaacc agtgccacat cccacttgaa tacttcttcc ttcaggacag gcttgacctg
     5401 agcggcaaag atcactgcca gcaacaccgc aaggccatgc agcagcagcg agaaccccca
     5461 ggcggggagg agaaatctgt tctggtctgt agacgtcata cggacaccac gagaaaggga
     5521 acgctctgtc cgacgctgca gatcggcgca cactgcgacc gcaacgtttg acacaggtcg
     5581 aactatatcg tgacgtctgt cggagggccg cgggaagaga gaagggagga gaagccggag
     5641 gaaaaatcgg gcagtgctga acattccacg cgcgagacgg gggagcgttc gatgagatcc
     5701 ggagcggtga ccgtgtcgag gacctgccct gcggcgcaca tccatgcgca gagcactgtc
     5761 gcatgcgtcg atttttgatg atgggcgtgg tgagattcgt gggagatggc ctgcgccgag
     5821 cccagtcctc caatgacgag gagacacagg acgagaacaa cggcaagcga ctttttcggc
     5881 gaccaagaca tcaataggct caacgagaca agatccaacg ctgggatggt agctaaaccc
     5941 acggtacagc gtcaagcctg agtatcggaa gcccttgtct tcccgatagg attcagaaaa
     6001 tttttccggt atactgcctc gatcgcgttg ctgttcacac cgatttttgc ctgtagagct
     6061 cgatgctgac acagatcctc aacttcattt tcggcagcaa gaacgaccgt gaaatcaagg
     6121 gcttgatgcc cgttgtccac cgcatcaacg gtttggaatc tgacctgatt ccgctctccg
     6181 atcaggcctt agccgacaag actcagacct tcaggaagcg gctggccgcc ggcgaaaccc
     6241 tcgacgatat tcttccagaa gccttcgccg tctgccgcga gatgtctcgg cgcaagctga
     6301 acatgcgtca tttcgacgtc caactgatcg ggggcatgat tcttcacaaa ggccggatct
     6361 ccgaaatgaa aaccggcgaa gggaaaacgc tcgtcgcgac acttcccatt tacctcaacg
     6421 cccttgaagg taagggcgct catctcgtga cggtcaacga ctatctggcc aaacgggatg
     6481 cgcaatggat gggccagctc tatcatgcac tcgcgctgtc caccggagtc attcagcacg
     6541 acgcctcgtt catctacgat ccgacctacg atgcctccga taagcgcctc cagcacctcc
     6601 ggccttgtac caggtacgag gcctaccgcg ccgacattac ctatgggacg aacaacgagt
     6661 atggcttcga ctatctccgc gacaatctgg tcgtgaccgc attggatcaa tgcgtacagc
     6721 gggacctgaa tttcgcgatc gtggacgagg tcgacagcat cctcatcgac gaagcacgga
     6781 caccgctgat catctccgga cccaccgacc agagcaccga tctctactac cgcatcaacg
     6841 ccattattcc gcagctcaag ccggaacacg actatacaat cgaagagaaa accaagacgg
     6901 ccgccctcac cgaggaaggc aacgtgcgcg tagaaaagct cttgggagtc gacaatcttt
     6961 acgatccggc caacatggac ctcgtccatc acgtcgtcaa agcgctgcag gcttatgcgc
     7021 tctacaagcg tgatgtcgac tatgtggtga aagacggcga ggtcatcatt gtcgatgaat
     7081 tcaccggccg attgatgccg ggccgccgct ggagcgacgg actgcaccag gctgtcgaag
     7141 cgaaagaagg cgtcaagatc gccaatgaga accagaccct ggcctctgtg actttccaga
     7201 actacttccg catgtacaag aaacttggcg gcatgaccgg caccgccgac accgaggcgg
     7261 cggagtttgc gaagatctac aacctcgacg tgaacgtggt ccccaccaat aggaaaatga
     7321 ttcggctgga ctacgccgac gtggtctacc gcaccgaaaa ggaaaaattc gccgcaatcg
     7381 tcgaagagat caaggactgc caccaacgag gccaaccggt tttggtgggc accatctcta
     7441 tcgagaaatc ggaaaagatc gccggcatgc tgagccgaaa cgggatcaaa cacaacgtgc
     7501 tgaatgccaa gcagcacgag cgtgaagctg aaatcgtggc gcaggccgga cggaaaggcg
     7561 ccgtaacgat cgccaccaac atggcaggtc gcggaaccga cattctcctt ggtggcaatg
     7621 ccgacttcat gttcaagcag gtgctgtacc gggaagaaca actggcggag gaacgaaagc
     7681 tcgccgtcta tgaagagatt cgtgccgact gcgaacagaa caagcaggag gtggtctccc
     7741 tcggcgggtt acacatcctc gggacagaac ggcatgagag ccgccgcatc gacaaccagc
     7801 tgcgcggacg cgcgggccgt caaggcgatc cgggttcctc ccggttttat ctgtcgcttg
     7861 aagatgattt gatgcgtatt tttgcctccg agagggtgtc gcaactgatg ttgaagctcg
     7921 gcatggagga aggcatcccg atcgagcacg gcatggtcac tcgcgcgatc gcgaatgccc
     7981 aaaagaaggt cgaggcgcac aattttgaaa tccgaaagca actcctcgag tatgacgacg
     8041 tcatgaacaa gcagcgggag gtcatctacc aacaccgccg ggcggtcttg gcaggcgaaa
     8101 acctcagcga cgacttgcgc gaaatgatgg aaaacttggt cgagtccgca ctcaatgtgt
     8161 actgccccgc tgaacaatat cccgaagaat gggacatgaa agggctggcc gagatgatgc
     8221 agggccagtt tggactcgac atcacgcagg gcaagcagga cggcggtgaa tctctccgtg
     8281 acgtgggacg agacgcgctc cttgaagatc tgaaggccca gatacgcgac ggctatgacc
     8341 gaaaggaaaa agaacttggg ccagagctca tgcgcttcct ggagaaaact ttcatgctgc
     8401 aggtgatcga ccaccattgg aaggaccacc tgttgggaat ggaccatctg cgtgacggca
     8461 tcggcctccg gggctacggc cagaaagatc cgctcatcga gtacaagcgg gaaggatacg
     8521 atctgttcgc gggcatgatg gaacgcatca agtcggatac gatcgagcgg ctcttccttg
     8581 tgcaggccgt tcgggaaggc gaacgctccg ccccccctcc gtccgtcctg tcacggcctc
     8641 agcccaagct cacattgaac cgcggggaag aaccggtgtc cacgccaacg gtccatcgca
     8701 gcgacgacaa agtcgggcgc aacgaccctt gtccgtgcgg ctcagggaaa aaatataaaa
     8761 aatgccacgg ggcataagga tcaggatgca agcccggctc cgcttacccg accatgcgac
     8821 cgctcgtatc tcacagttgc ttggcgcgca gccccttgcg agtgacttgc cggcaattcg
     8881 tgtcgaacga ccgcgactcg acttatttct tctcctttct agctaataag gggaagctcc
     8941 ctggctctcg tttttgcgaa tcgcgagatc gattcgtcct tttccctccc tcattatcca
     9001 tgtaggcaag atccctcgcc taattcgcga ttttcccgac agctcaggca ctattccttc
     9061 gaagcacaac gactctccgc aggcgagtca tgccgatgtt tgaagtttcg tcccgtgagg
     9121 cgtcggacga aacaagtttt ggcgtcaagc tgtgcctgag acgaacaatg gtcgagttga
     9181 tgggccaaag cggattcagc gcagcaattc cgccttatcc cgccttgact tagcgcactc
     9241 ctagaggcta ctctacacga cttttggacg ccccaccatt atgggtttag ccacccaggg
     9301 ctcggggtgt cccttttatt atggattttc ccggagttct tcgtgagcac cggccatcct
     9361 caactcgtcg ccgctattgc atccgaaatc gcacgtgagg gtcccatccc tttcgtccgg
     9421 tttatggagc ttgccctcta ccatccgcaa ttcggttact acatgcgctt ccccgagggc
     9481 ggcgcagagc ggataggttg gaatggcgat ttttatacaa gctcagacgt tcacccaatc
     9541 ctaggacaag ccatcgccag gcaagccgca caaattgatg ccctgctggg atatccgaat
     9601 ccttttacag ttgtggagat gggaccgggt aaaggcctcc tggccagtca cgtgttgtcg
     9661 gggctccaac ttgctcctca gtcgctggca caccgcctgc gatacgtcct catcgaatgc
     9721 agtccggcga tgcgcgcaat gcagcaacga cacctcgatt cctggctcag gcaaaccggt
     9781 tgcgtgagtt gggtggaaga tgtcaacagc ctcgcgagag aaagcgtgcg cggcctgatg
     9841 ttcagcaacg aattacccga tgccttccct gtccaccgag tccaacaaaa ggaccacgcg
     9901 ctctgggaag tcttcgtgga cgttcgggac ggcggttttg tggaatgtct gaaaagcctc
     9961 tca
//
LOCUS       DASTQY010000448         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1516705, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000448 DASTQY010000000
VERSION     DASTQY010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516705"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="VFR82_14405"
     CDS             complement(<1..1310)
                     /locus_tag="VFR82_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502841.1"
                     /translation="MVGWYDPPQLLRTAGQVVTSTIFGRHADYRLIEALYDDPDDLPD
                     DDPTHFIIDYSQHKEFWLDYVADLGEGWNSTYAVAYHSSRPKLDLNWEKNLYVTVRGH
                     VILFGGDEVYPTASRLAYKERTFHPYECAYNGDKARPDMFAIPGNHDWYDSLVAFTRY
                     FVDKDEIAGFPTPQLRSYFAMKLPHGWWLLALDTQLTSYIDGPQVKYFQLVAKQIPDG
                     DSIILCNSEPTWFYEAEYLRYDLNVNDRNLDFVEKEILKGKSVQVFLAGDLHHYRRHE
                     ARDGTQKIIAGGGGAFLHPTHGWKVNDLVETLPPAQADAARTFHHKVSWPNPTVSRLL
                     SLRNGLFPLVNWKFGIAPASLYLLTAWSVMAKVGDTSDVTEAASRVLEKLLLTPFPIF
                     WTAVLFLGFFLFTDTYVKWYRWTAGTMHAVSHIAATFFIGWGAS"
     gene            1775..1993
                     /locus_tag="VFR82_14410"
     CDS             1775..1993
                     /locus_tag="VFR82_14410"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEU4502842.1"
                     /translation="MAFDRIKVDPNVCGGKPTVRGLRITVDFVLKLLGDGYTAKQIIG
                     EYPELELDDVYQAAKYGAWLASERTTAV"
BASE COUNT          634 a          683 c          738 g          662 t
ORIGIN      
        1 tatgaagcgc cccagccgat gaaaaatgtg gccgcaatgt gcgacacagc gtgcatcgtt
       61 cctgcggtcc agcggtacca tttcacatag gtatccgtaa aaaggaaaaa gcctaggaag
      121 agcacggccg tccaaaagat cgggaatggt gtgagcagca atttttctag aacccgcgac
      181 gccgcttcag tcacgtcgga tgtgtcgccg acctttgcca tcacggacca tgccgtcaat
      241 aagtacagac tggccggtgc gataccgaat ttccaattca caagaggaaa aagaccatta
      301 cggagggaga gtagtcgtga cactgtgggg ttcggccaac tgactttatg atgaaatgtc
      361 ctcgcagcat cagcctgcgc aggcggtaac gtttctacca ggtcattcac cttccagcca
      421 tgggtcggat ggagaaatgc gccacctcca ccggcaataa ttttttgggt accatcccgc
      481 gcttcgtgtc ggcgatagtg atgaaggtct ccggctagaa acacctgaac ggatttacct
      541 ttgaggatct ccttctccac aaagtcaaga tttcggtcat tcacattgag gtcgtaccgt
      601 aggtactcag cctcatagaa ccacgtcggt tcggaattgc acaggataat gctgtcccca
      661 tccgggatct gtttggcgac cagctggaaa tattttacct gtgggccatc gatgtatgac
      721 gtgagttgcg tgtccagcgc taagagccac cagccgtgcg gaagcttcat cgcaaagtaa
      781 ctgcgcagct gtggggtcgg aaatccagca atctcatcct tgtcgacaaa ataccgtgtg
      841 aaggccacta agctgtcata ccagtcatgg tttccaggta tggcaaacat atcggggcgg
      901 gccttgtcgc cattgtaagc gcactcatac ggatggaatg tccgctcctt gtaggccaat
      961 cgactggcgg tgggatacac ttcatcgcca ccaaagagaa tcacgtgacc acgaactgtc
     1021 acatacaggt tcttttccca gttgaggtca agcttggggc gactgctgtg gtaggcgacg
     1081 gcataggtgg agttccagcc ttcccctaaa tcggcgacgt aatccaacca aaattccttg
     1141 tgctgcgagt agtcaatgat gaagtgtgtt gggtcatcat ctggcaaatc atctggatca
     1201 tcatagagcg cttcgatcaa ccggtaatct gcgtgccgcc cgaagatcgt agaagtgacg
     1261 acttgtcctg ctgtgcgcag cagctgaggc ggatcgtacc atcccaccat ttggtgtctt
     1321 ttaagcgaga tgctgtcgtt gttggatggc gttcgtgcct tgagaggcgt ggatggcttt
     1381 gtggtgaggg ggtggttgat tggtgctgag cggtcagctt cagacatgcg ggagtgtacc
     1441 atagcctctt gccaaagaag aacgatgatt aagcatcggc tattaagaag aagaccaaga
     1501 aaggccgccc agggagggtc aggcaggaac gtcgacgtat tcaacttgac ttatgtcaaa
     1561 gcgttcgaca agcggagcaa ggagccaacc cgctctttcg aacaggtcct caaagatctc
     1621 aagcgtgacg gactcttata gtctcgtcgt caagaagtct gcagcacggg agctgcgtgc
     1681 aatagatctc tgagagatag ttgtttcctg gcagagagct tcatctatag ccctttacgc
     1741 ttgcgtctct gattagaaac gagtaccgta actcatggca tttgatcgca tcaaagtcga
     1801 tcccaacgtc tgtggaggga agccgacagt gagaggcctc cgcattacgg tggattttgt
     1861 tctcaagttg ctcggcgatg gatatacggc aaaacagatt atcggggaat atccggagct
     1921 tgagcttgac gatgtgtatc aggcggccaa atacggcgcc tggctggcca gtgagagaac
     1981 gaccgctgtg taatgaggct tcttgccgat ttgcatatct cacctcgcac cgttgaatat
     2041 ctacagtctc tcagtcatga cgttgtccgt gtatctcagg tgcttcctcc gacgagttct
     2101 gatcaccaaa tcatcgcata tgcagtcagt gatcgtcgtg ccattctgac gcaagatctg
     2161 gacttttctg ctgagattgc agtatcggga caagcctttc cttctgtggt tctcttgcgc
     2221 ctctcatcct ctcgtgttga aactgtgaat gctgttcttt ccgatgtcct gccgacattg
     2281 gaacaggacg ttaaagacgg cgcgctaatt accgttgaag accaccgtct ccggcgacgg
     2341 agattgcctc tctcgtctgc gtagtctaaa aatcttttga ggaatctgaa aataaagaaa
     2401 ggccgcgctc caatgttatt ggagcgcggc ctcagagcgt gaaggcaacc ggtcagctcc
     2461 tggaagggtc aggaccagaa tctgcttggc catcggttgg cgcttgtcag cgacggcctc
     2521 tggtcggctt cacacctcct cgttggtcgg gttttggcct atggcttatc gcacgaaacc
     2581 acgagcgagt gatcgtctcg ataagcgatc ggacaaaacc tgaatgtcgg cgctgctagg
     2641 gcgtcttcta ctggtctcag cagtccgcac tcatgattca gcattgattc gtttagtctt
     2701 gccagatgcg agtcacg
//
LOCUS       DASTQY010000449         7103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1718924, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000449 DASTQY010000000
VERSION     DASTQY010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718924"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VFR82_14415"
     CDS             <1..921
                     /locus_tag="VFR82_14415"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU4502843.1"
                     /translation="QGGHWTIVEHVLGLLTEAEEVRPDLNEAHKKKQYNMFDQLGSSD
                     LVTLIKQYLNSADKPRTEGLETVLLMMKPSAMPALCSLLGDLEHAAHQALIANALVEL
                     AKDSPEIIVKHLSDRRVTFVRNLLGIITRWNNPKLSEAVEKILRHPDPLIRREAIRTL
                     AGLKPSGSAAKLVPLLNDQDEGVRLATLKPLLTGHYTATFSVWEPIVNAENFNEREAA
                     EKRNIFQAMRVTVGDEAVPYWATLLTDWGWTNRKKREDLALLAVDALGKLGTAAAKAA
                     LDTGSKKGTAAVKQACTAALTGTNKQARTA"
     gene            951..2459
                     /locus_tag="VFR82_14420"
     CDS             951..2459
                     /locus_tag="VFR82_14420"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU4502844.1"
                     /translation="MADPLRASDGPAPNAPAVQPILTHEKSLTKKVAKGSEAGDIYDQ
                     QILMLGTQLVTQVSILIKMSRLHGRTNSALESPVESALTVIKTLAQDEPISVRLQNDF
                     LFLGQRHLKVTAAQMPVFSTVIDTFNQWRIGGVSFSKDVESRDFREFIFQLLAFDAAT
                     SSIATLRTALEQKAVKGIEIEELRVLRGKATAKTRYANAASAVGTLVQDSREKGTIGF
                     KQAKRAIQSIVDLMMNDEASLLGLTTLRCHDQYTHNHSVNVSLLSMALGNRAGYPKVE
                     LADLGLAALFHDLGKATIPMEVLNKPSEFTEEEWAMMRNHPTEGALSLAKMRGITNLP
                     GRMASASFEHHMNMDFSGYPKLNLSWKISMTGRILMIADCYDAMTSSRVYRREPMSPS
                     KVLGFMFAKAGTSFDPTLMKLFMTCVGIVPIGSLVLLDSNELAVVLKPAVDPSEAEYP
                     FVKVITDAQGEAIDNGREIDLREKNENGDYRNYIVRLIDNSEHKFDTSRYFV"
     gene            2592..3158
                     /locus_tag="VFR82_14425"
     CDS             2592..3158
                     /locus_tag="VFR82_14425"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEU4502845.1"
                     /translation="MRIAKTLGGQTVLVLAAVGLWVLSLTGAGVAAAAEFKMGVIDPQ
                     AVLEKSKAGKKALDGLKEYVSTRQKLLSRDEEELRNTEKTLKEAGAKLSEGEKKEKEA
                     QFRSKIQDYQKRAQEFNQELQGKQKELVDEYMKRIASATQTVAEKGGFSIVVDKGSEQ
                     TIRIVIYSKDAIDLTDQVIKEFDRVNSK"
     gene            complement(3198..3566)
                     /locus_tag="VFR82_14430"
     CDS             complement(3198..3566)
                     /locus_tag="VFR82_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU4502846.1"
                     /translation="MKHNPGFLKLVEAARARVKACTVAEAKARLDGGEVTHFFDVRED
                     HEFGKDHAKGARHLGKGIIERDIETLVADKRASIVLYCGGGYRSALAADALQQMGYTN
                     VTSMDGGIKAWREAGYPMES"
     gene            complement(3585..4598)
                     /locus_tag="VFR82_14435"
     CDS             complement(3585..4598)
                     /locus_tag="VFR82_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEU4502847.1"
                     /translation="MRAMSNPPNPYESTHRDLLEPASSARLELYDDATREILSRNDSP
                     DLSFRWSVNPYRGCFHACAYCYARPTHEYWGFGAGTDFESKIVVKRNAPELLRRAFEK
                     PSWRGELIVFSGNTDCYQPIEASLELTRACLEVCAEYRNPVAIITKGVLVLRDLDILK
                     RLQQEASVRVYCSIPFADDEVARRVEPQAPTTRKRFDAMARLSDAGIPTGVSIAPIIP
                     GLNDEDIPDLLHRARQAGARQAMHTLLRLSTSVESVFIERMAEAFPERIGKITHRIKE
                     VRGGAMSDSAFFSRHHGQGPYWNAIERLFEVAKRKAGFEESDDDHVPATFRRPGREQA
                     RLF"
     gene            4713..5999
                     /gene="serS"
                     /locus_tag="VFR82_14440"
     CDS             4713..5999
                     /gene="serS"
                     /locus_tag="VFR82_14440"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU4502848.1"
                     /translation="MYDLRDLRDNLDQIKSRLGTRGRDVQWDSLRKLLEERRGLTVQV
                     EQLRADLKKGSDEVATLKRQKLPSDAAVAAMKQLGERIADVESALRTIEEHLDEIALR
                     IPNLPHQSVPAGTGPEQNVEVRRWGAPPTFSFPPQTHWDIGERLGILDFDRAAKIAGA
                     RFAVLTGDGARLERALINFMLDLHTTEHGYREVLPPLMVNRSAMTGTGQLPKFEEDLF
                     RLRDEDYFLIPTAEVPLTNLHRDEILSESALPIRYTAYTPCFRREAGSYGKDTRGLIR
                     LHQFNKVELVAFTKADHSYEELERLTGHAETVLQRLGLHHRVVTLCTGDMGFSAAKTY
                     DLEVWLPSQNQFREISSCSNFEAFQARRAAIRCKGAGGKKDSKTEFVHTLNGSGLAVG
                     RTLVAILENYQQPDGSIIMPEALRPYLRGMDRIKRE"
     gene            6032..6121
                     /locus_tag="VFR82_14445"
     tRNA            6032..6121
                     /locus_tag="VFR82_14445"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6066..6068,aa:Ser,seq:tga)
     gene            complement(6299..6505)
                     /locus_tag="VFR82_14450"
     CDS             complement(6299..6505)
                     /locus_tag="VFR82_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502849.1"
                     /translation="MSEWHLVSVLVALVIGFVSGLIYALRLYKRIEADCDREWAKRQG
                     MREMKPQYGELPVRINPILRHLRL"
BASE COUNT         1665 a         2042 c         1915 g         1481 t
ORIGIN      
        1 cagggcggac actggacgat tgtggagcac gtgcttggct tgttgaccga agcggaggaa
       61 gtgagaccgg acctcaacga ggcccataag aaaaaacagt acaacatgtt cgatcagctg
      121 gggagctcgg acctagtcac actcatcaag cagtatctga acagtgctga caagcctcgg
      181 acggaaggac ttgagactgt gctgttgatg atgaaaccat cggccatgcc tgcgctctgc
      241 agtctcctag gcgacttgga acatgcggct catcaggccc tgattgccaa cgccctggtc
      301 gaactggcaa aagacagccc tgagattatc gtgaaacatt tgagcgatcg ccgcgtgacc
      361 tttgttcgca atctccttgg catcatcaca aggtggaata atcccaagct gtcagaagca
      421 gtggaaaaga tcctgcgcca tcccgatccg ctgatcaggc gagaagcgat cagaacgctg
      481 gccggcctga aacccagcgg cagcgctgcc aaactcgtgc ccttattgaa cgaccaggat
      541 gagggcgtgc gccttgccac actcaaaccg ctgctgaccg gtcactacac ggccacattc
      601 agtgtctggg aaccgatcgt caacgcggaa aacttcaacg agcgggaagc cgccgagaag
      661 cgaaatattt ttcaggccat gcgtgtcacc gtcggcgacg aagccgttcc ttattgggcc
      721 accttgttga ccgactgggg atggacaaac cggaaaaagc gtgaagacct cgcgttactg
      781 gccgttgatg ccctggggaa actgggaaca gccgccgcaa aggccgccct cgatacgggt
      841 tcaaaaaaag ggactgcagc agtcaagcaa gcctgtaccg ccgccctcac cggcaccaac
      901 aaacaggcac gtacggcctg aagcaatgag agacagaagg atgtccttcc atggctgatc
      961 ctttgcgcgc atctgacggt ccggccccga atgccccggc ggtccaaccg atcctcactc
     1021 atgaaaagtc gctgaccaag aaggtcgcca aagggtcgga ggccggcgac atctacgacc
     1081 agcagatttt gatgctgggg acgcagctgg tgactcaagt cagcatcctg atcaagatgt
     1141 cgcgcctaca cggccgcacg aactcagcgt tggagagccc ggtggagagc gcccttaccg
     1201 tcatcaagac gctcgcacag gatgaaccga tctcggttcg gctgcagaac gatttcctct
     1261 ttctcggaca gcggcacctg aaagttacgg ccgcacagat gcccgtgttt tcgaccgtca
     1321 tcgatacgtt caaccaatgg cgtatcggag gtgtgtcatt ctccaaggac gtcgagtccc
     1381 gggattttcg cgaattcatt tttcagcttt tggccttcga tgcagccacc agttcaatcg
     1441 ccacgcttcg gaccgctctc gagcagaagg cagttaaggg catcgagatc gaagaactgc
     1501 gcgtgctgcg cgggaaggcc acggccaaga cccgctatgc caatgcagcc tcggcagtcg
     1561 gaacgttggt acaggattcg cgtgagaaag gaacgatcgg cttcaagcaa gccaagcgtg
     1621 ccatccaaag catcgtcgat ctcatgatga acgacgaagc ctccttattg ggcctcacga
     1681 cgcttcgttg tcacgatcaa tacacgcata accattcagt caacgtatca ttactctcaa
     1741 tggctctcgg caaccgggcc ggctatccga aggtcgaact cgccgatctg ggcctggccg
     1801 ccctctttca cgacctcggc aaggcgacga tccccatgga agtgttgaat aagcccagcg
     1861 agtttaccga agaagaatgg gcgatgatgc gtaatcaccc caccgagggc gcactgagcc
     1921 tggccaaaat gcgtgggatc acaaatcttc ctgggcggat ggcatcggcg tcattcgaac
     1981 accatatgaa catggacttc tcgggctatc ccaaactgaa cctttcttgg aagatttcca
     2041 tgactggtcg catcctcatg atcgccgact gttatgacgc aatgacctcg tctcgcgtgt
     2101 accggcgaga gccgatgtct ccttccaagg tcctcggttt catgttcgcc aaagccggca
     2161 catccttcga cccgacgttg atgaaactat tcatgacgtg cgtcggcatt gtccctatcg
     2221 gcagcctggt cctcctggat agcaacgaac tggccgtggt cctgaagccg gctgtcgatc
     2281 cgtccgaggc ggagtatccg tttgtgaaag tcattacgga tgcacaaggc gaggccatcg
     2341 acaacggccg cgaaatcgat ctccgggaaa aaaatgagaa cggcgactat cgcaactaca
     2401 tcgtccgctt gatcgacaac tccgagcaca aatttgatac gagccgctat tttgtttgac
     2461 gacccggctt gccactcgtg gcggtctccg ccgcgagcgg catcgggggc tccttgacac
     2521 tgccattcac cgccgttatt ctttcctttt ctcaacgagt ttagctactc atccactaag
     2581 gagccgacac tatgcgtatc gcgaagaccc ttgggggtca gacggttctc gtccttgcgg
     2641 ccgtcgggtt gtgggtactc agtctgacgg gcgcgggcgt ggccgcagcg gcggaattta
     2701 agatgggggt cattgatccc caggccgtac tggaaaaatc aaaggcggga aagaaggcgc
     2761 tcgatggtct caaagagtac gtctcgacgc gacaaaagct cttgtcgagg gatgaggaag
     2821 agctgcgcaa caccgagaag acgctcaagg aagccggcgc taagctcagc gagggagaga
     2881 aaaaagagaa ggaggcgcag tttcgcagca agattcaaga ttatcagaag cgagcacagg
     2941 agtttaatca ggaactccag gggaaacaga aagaactcgt agacgagtac atgaagcgga
     3001 ttgcatcggc gacgcagacc gtcgccgaga agggtggttt ttcgatcgtc gtcgacaagg
     3061 gcagcgaaca gaccatcagg attgtgatct acagcaagga tgccatcgac ctgaccgatc
     3121 aggtcatcaa ggaattcgat cgggtaaaca gcaagtagag acgcccagcc tgacgggctt
     3181 ctcgcggaga ggttccgtca ggattccatc ggatatcccg cttccctcca cgccttgatc
     3241 ccgccatcca tcgaggtgac attcgtgtag cccatctgct gcagtgcatc cgcagcgagc
     3301 gcggatcgat agcctccccc gcaatacaga acaatcgaag ctcgcttatc ggcaaccagc
     3361 gtctcgatat cccgctcgat gatgcctttt cccagatgac gtgccccttt ggcgtggtct
     3421 ttcccaaact catgatcttc ccggacatca aaaaagtgcg tgacttcccc gccatcgagg
     3481 cgcgccttag cctcggctac cgtgcatgcc ttcacccgtg cacgcgcggc ctccaccaat
     3541 ttcaaaaatc cggggttatg cttcatgcgg tcatcgtttc gacgtcaaaa caaccgtgct
     3601 tgctcgcgtc ccggtcgacg aaacgtcgcc ggcacatgat cgtcgtcgga ttcctcgaag
     3661 ccggccttcc gcttcgccac ttcaaacaag cgttcgatcg cattccaata cggtccttga
     3721 ccatggtggc gtgaaaagaa agcgctatcc gacatggcac ctccacgaac ctccttgatg
     3781 cgatgcgtga tcttaccgat ccgctcagga aaggcttctg ccatgcgctc gataaaaacc
     3841 gattccaccg atgtcgacag ccgtaacagg gtatgcatgg cttgcctggc gccggcttgc
     3901 cgggctctgt gcaggagatc cgggatgtct tcgtcgttga gacctggaat gatcggggca
     3961 atggaaacgc cggttgggat tccggcatcg gacaaacgag ccatggcgtc aaaacgcttc
     4021 cgggttgttg gagcctgcgg ttcaacgcgc cgggccactt catcatcggc aaagggaatg
     4081 ctgcagtata ccctcaccga ggcttcctgc tgcaatcgtt taaggatgtc caaatcgcgg
     4141 agaaccaaca cacctttcgt gatgatggcc acggggttcc gatactctgc acagacctcc
     4201 aggcacgcac gggtcaattc gagcgacgct tcgatcggtt ggtagcagtc ggtgttgccg
     4261 gaaaacacga tcaactcccc tctccaggat ggtttttcga acgcccgacg caacaattcc
     4321 ggcgcattac gcttgacgac gatcttcgac tcgaaatcag ttcccgcccc gaagccccaa
     4381 tattcatgcg tcggcctagc ataacaatat gcgcaagcat gaaaacaccc tcgatacgga
     4441 ttgacactcc accgaaaaga tagatccggg ctgtcattgc ggctgagaat ttctcttgtc
     4501 gcgtcatcgt agagttcgag ccgagcagat gaagccggtt cgaggaggtc tcgatgtgtg
     4561 gactcatacg ggttgggagg attggacatc gcgcgcatgt tcttatggtc tctcttcggc
     4621 ctgccgctgt caacggggct cgcaacgcgc cgttcgttga ctcctcgcca cctcgatgtt
     4681 agatttcagc cgccatttgg aacgcgcctc tcatgtacga tttacgcgat ctccgcgaca
     4741 atttggacca gatcaagagt cgactcggaa ctcgcggccg agacgtccaa tgggactctc
     4801 ttcgtaaact gcttgaagaa cggcgcggtt taaccgtcca agtcgaacaa ctcagggcag
     4861 atctgaagaa gggctccgat gaagtcgcca cgctgaaacg acagaaactg ccgagtgatg
     4921 ccgctgtggc cgccatgaag cagctcggtg agcgcatcgc ggatgtggaa tcggcgcttc
     4981 gcaccattga agagcatctt gacgagatcg cgctgcggat tcccaatctt ccccatcagt
     5041 ccgttccggc aggaaccggt ccggagcaaa acgtcgaggt gcgccgttgg ggcgcgccgc
     5101 caacattctc gtttcctccc caaacacatt gggacattgg cgaacgtctc ggcattctcg
     5161 atttcgatcg cgcggcgaaa atcgccggtg cccgatttgc cgtcctgaca ggcgacggag
     5221 caagacttga gcgggcgctg attaatttca tgctcgatct tcacacgacc gaacatggct
     5281 atcgagaagt cctgccaccg ctcatggtga atcgatcggc catgacggga acgggacagc
     5341 tgccgaaatt cgaggaggat ctctttcgcc ttcgcgacga ggactatttt ttgataccga
     5401 ccgccgaagt ccccctcacc aacttacacc gagacgagat cctcagtgaa tcggcacttc
     5461 cgattcgata tacggcctat accccctgtt ttcggcgtga ggcagggtcg tatggcaagg
     5521 acacgcgagg cttgatccgt ctgcatcagt ttaacaaagt ggaactagtc gccttcacga
     5581 aagccgacca ttcatacgag gagttggagc gactcacggg acacgccgag actgtcctcc
     5641 aacggctagg acttcatcat cgcgtcgtaa cgctttgtac cggagatatg gggttttctg
     5701 ccgctaaaac ctacgacctc gaggtttggc ttccttcaca gaaccagttc cgcgagattt
     5761 cgtcttgcag caacttcgag gcgtttcagg caagacgggc tgctatccgc tgtaaaggcg
     5821 ccggcgggaa aaaggattcg aaaacagagt tcgtccatac cctgaatggc tcagggctcg
     5881 cggtcgggcg gaccctcgtt gcaatcctgg aaaactacca gcaaccggac ggatcgatta
     5941 tcatgcccga ggccttgcgg ccatatttac gaggaatgga ccggataaag agagagtagt
     6001 tagagtgttc gccggagttt cgtggcctag cggaggggtg acggagcggc cgaacgtgcc
     6061 ggtcttgaaa accggagacc tcgcaagggg tccgcgagtt caaatctcgc cccctccgcc
     6121 atagttcaat gcctcagtag acaacatagg actcacacct cggcatcaac cccttcttct
     6181 gaatgtaacg acaaaagcga gtagcagctc tctttaggtt tacagcacct ttttcaggtt
     6241 gaaggtaccg ttcatattgt cacaggagcg ccttcgcatg tccttccacg ctgcctggtc
     6301 acaatcttag atgccggagg atcggattga tacgcaccgg cagctcaccg tattgcggct
     6361 tcatctctcg cattccctgc cgcttggccc actcgcggtc acagtctgct tctatccgct
     6421 tgtatagccg gagcgcatag atcagaccgg acacgaagcc aatcactaac gcgaccaaca
     6481 cggacacaag atgccattcg ctcatgtaaa gtctcttgcg gtaaacttga agttgttctg
     6541 aacagtctga ctgtggcaag ggcggagtcg gcagcagaac gaagcgtctc cctgaggaca
     6601 cgcagacatt gtgggaattg aacctctccc ccacctacat tggactaacg ttcgactgcc
     6661 cagcaggtgt catctctgat atttcactag aacaactgct gtgagcgcca ttaaaggcac
     6721 tgacagcaaa gtagtactgt gcattaaact caagtcctcc gattgcggta gaaggctcgg
     6781 ccacatccac tgatttttca tagttgcatg atccaacact tccgcttgaa tgttttccat
     6841 aatgcacggt gtatgaaatc accgtaggat cgttcacagg gtcccaggct actgatgcta
     6901 tggcgccggc accgcttcct tctccccctt ctccacatcc agctaacgag atgaggtaaa
     6961 atccgaacgc aacagaacag acaaacctgc atgcaaagac catgggttct cccccaagtg
     7021 ttctagttta agaccgaggc ttgaaacgtc agtggcgtat gcaaggacca ctagcgcttg
     7081 ctcggtcaga ttttgacctt gcc
//
LOCUS       DASTQY010000450         7653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1114504, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000450 DASTQY010000000
VERSION     DASTQY010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7653
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114504"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..1326
                     /locus_tag="VFR82_14455"
     CDS             343..1326
                     /locus_tag="VFR82_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502850.1"
                     /translation="MQRQTNPDASERALDEAAARTVVEAFTYMLDHRKDYPRFDESMK
                     KQALQEVVIEPTVINQEGKSFPFLVARTAQSGQVRLLISAEFLRKQGHLGHPETLVPA
                     LAKEFQWVVSKADTGTKLQPPSVRSDLAKVPIRTDQEIATMSAEERLNLLRALLGRYL
                     NTVDEHNSLDGQPHYEAGTNTLIQPAQPDSTTKLYDIRVREALQKIVHGPWFMTQTPK
                     AVRCLLNGTIWNVAFVKIDQRDWATRTRVLSKPVVVGEAGRTIQPATILVNTFRLAVP
                     DDPFYGDTNGLPMGALSADQVARVIAKEIEQNIIEKSMRGHVAQDELTMPK"
     gene            complement(1521..2102)
                     /locus_tag="VFR82_14460"
     CDS             complement(1521..2102)
                     /locus_tag="VFR82_14460"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4502851.1"
                     /translation="MVVNEKAWNGRCVPRIPLQASINYLTPEILGKGQVIDVSREGLR
                     IESQDPVQIGMRLALVLYLSHDQEPVMIEDATVQWTIGTHFGVKFVKWSTNAQTRLTN
                     FFWTGIQEKCDSLRDLMTEAVQASPLTESMDLQATPREDAQRDLIRRTLEACRWVIGG
                     ASGAAKKLGMKRTSLQYRMKKLGITRTKPVSES"
     gene            2692..4281
                     /locus_tag="VFR82_14465"
     CDS             2692..4281
                     /locus_tag="VFR82_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU4502852.1"
                     /translation="MTESQHHLKGFQFIVLNLVIGLAHMVVLFNAGSYVALLPHAAGN
                     LGGVLPSFGTWAQTDFMIALALAFPLARWLSSRFGETRVFVVSFIVYAMASGLCLLGS
                     EISTFLPARVLLGLAGGLTLPLGQSLLLKEYPDRIKSLGLAIWGLFTLMPFTAGLATG
                     GWVADELGWRGLFALNIPFALLIASVSTILLPHHSVVSQVERFDFVGFALLALIFAGI
                     QTIFNLGNDFDWFDSSVIQVTMVVVVAAIPVWIIWELGEERPAVNIRLFLHRNFTVGL
                     VSLSLGFLSIQGLLALFVVQLQTVLGYSSTLAGLAFLPMLLFGVPLIAIMHEVAKRFD
                     VRMLACFNAVGFAATFYWIGLFDDPQSYDQIFWPMVLEGVFLGSFFTPLTILTLHGLS
                     GEEMMSAAEVANLFRLAAGAIGITWQSVVVFRRIPFHQLALSDHFGGRISASYDAWSQ
                     IVEKLQAIGFDRAGTERHLQLLIKQHAGILALNDAFLLSSCLCLGVAVLVWFARSSRI
                     PASTEPDAVQAWQAEELMEQP"
     gene            4278..5783
                     /locus_tag="VFR82_14470"
     CDS             4278..5783
                     /locus_tag="VFR82_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEU4502853.1"
                     /translation="MKPRWITRQVLKNASWAVFWMVSGCAWIPKGDPPAAYIEPPELK
                     ETLAEVTSRLHNWPEDHWWELFGNPELNQLVETALKDNPGLKRAAARLREAQGLVRVE
                     GARLLPFLEADASLTYERISQHGVFAALNPEVAGARILLGIVNPLSFRYEFDFWGKNR
                     ARLEGALGQAAAEEAERAEVHLRLSTGIARAYLRGQALHRQLELAASIVDLRHHLRRL
                     AETRYKLGLDNDLAVKQAVADYEAAVKRRAGVRDQLDVQRHLVARLTGKGPDDVQNLF
                     AKSSIAGLNEVPIPEHLSIGLLVHRPDLAASLYRVDSAARLVKAAKAEFYPTIDLTAF
                     VGFNALTLTKGADQLANLLFSGQSFSYGIAPGIRLPIFEGGRLRGELTVHRAEYDAAV
                     ELYNETLLDALREVADSLSAWKATNDMLESHERLVASLNQDWHLAKVRVATGLDDDRE
                     ALRHRYPLLEQEYTLRALESDRLVAAVDLIEALGGGYHNPDLAKRPAHNES"
     gene            5786..7222
                     /locus_tag="VFR82_14475"
     CDS             5786..7222
                     /locus_tag="VFR82_14475"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU4502854.1"
                     /translation="MSTTHSVPPPAPHLYEGSLHAQRNRRLFMVLIVMMLLGLAYAVY
                     WWTTGRHWIRTDNAYVTGNLVPVTAQATGIITQVLVEETQFVHRGDVIIRLDEHEAYA
                     ALGRARGRLGESVRRINALFLTQRQLTEKLRARRARLNVVWHDVDRYKKASPSGAVSK
                     QILQNALDQLQALEAEVRETQAEYDTLDAQIGGTTVMEHPAVELAKHEFINAHLEYAR
                     QQILAPASGYVAKRKAQVGDRVRPGTNLITIVPLDHLWVEANLRETELEHVRPGQPAE
                     IRVDLYGDRHIFHGTVEGLVPGTGSPFALLPPDNSTGNFIHITERVPVRIALAADEIR
                     DHPIRPGLSTFTKIRVTESGQSVWSSLAKADTEEYQTDVYSDELTGAEALALSVITGN
                     VRSDRLHTPEMAEGTGTILGENHRHRFSERSKPKSNVPLRSPDLGEAFLPLSPSIGPG
                     SDRLGPEAGPSTMRPRRPDSTRPQSPSQ"
BASE COUNT         1687 a         2072 c         2105 g         1789 t
ORIGIN      
        1 tgacaggtct atagcgcgtc gggtcattga gaccgaatga ggtggcgctg cggcagacgc
       61 cggactaatc ttcgaatggt caaaagccgt gctatgaggg ttctcgaaac ccatactata
      121 atgaagaagg gcgactgcaa aaacatggac gtggagaccc tgcctgtgtg gaacatttct
      181 ggaataacaa gcgtggtagt ctctttggtg tgtatcccct taacgacggc cgtaccagtt
      241 caagcgctag caagccccca agtccaagaa cgccatagta aaaggccggc gaccgaaccg
      301 gtaagcactc agtattcaat cccgggtgcc tccttcctcg tcgtgcagcg tcaaacaaat
      361 cccgatgcgt ctgaacgggc gctcgatgaa gcagcggctc gaacagtggt agaggccttc
      421 acgtacatgc tggatcatcg aaaggactac cctcgctttg atgaaagcat gaagaagcag
      481 gctctacagg aggtggttat tgagccgact gtgatcaatc aggagggaaa gtcgtttccc
      541 ttcctggtcg cccggaccgc acagtcaggc caagtacgcc tgcttattag tgcagaattt
      601 ctccgcaaac agggtcacct tggacatcct gagacgctag tcccggcgct ggccaaagag
      661 ttccaatggg tcgtgagcaa agcagatact ggaacgaagc tccagccgcc ttcggtgcgc
      721 agtgatcttg ccaaggtgcc gatccgtacg gaccaggaaa tcgctacgat gtcagccgag
      781 gagcggctca atctgcttcg tgcgctcttg ggccggtatc tgaataccgt agatgagcac
      841 aacagtctcg acggccaacc gcattacgag gcagggacca acaccttgat ccagccagcg
      901 cagccagatt ccaccaccaa gctgtacgat attcgggtcc gagaggcctt acaaaaaatc
      961 gtgcatgggc catggttcat gactcaaacg ccaaaagcgg tccgctgttt gctgaacgga
     1021 actatctgga acgtcgcctt tgtaaagatt gatcaacgtg attgggccac acgtacccgt
     1081 gtgctgtcca agcctgtagt ggttggggaa gcaggtcgca cgattcaacc ggccacaatc
     1141 ttagtgaata cattccggct ggcggtgccg gatgatcctt tctatggtga tacgaatggc
     1201 cttcccatgg gtgccctgtc agcagatcag gtcgcgcgag tcatcgcgaa ggaaattgaa
     1261 cagaatatca ttgagaaatc catgagaggt cacgttgccc aggacgagct gaccatgccg
     1321 aagtgagtgc cggaacggca taaccctccc ctgtcagtcc gcattccgat acttcgttga
     1381 cgattgtgcc gtatgacgcg gtgtaggaat tccgaacccc aatctcagac aacaattgga
     1441 tagatgggcc cgggttgtac tgtcgtggta gcagcgtggt agcagatgag gagagccgga
     1501 gcccgagatt tttagaaatc ttaactttcg gagactggtt tagttcgagt aatgcctaac
     1561 ttcttcatcc tgtattgaag agaggtgcgt ttcatgccga gctttttggc ggcaccggac
     1621 gcgccgccga tgacccatcg gcatgcttcg agcgtccgac ggatcagatc acgctgggca
     1681 tcttcacggg gtgtggcttg gagatccata ctctcggtga gaggcgatgc ctggaccgcc
     1741 tcagtcatga gatccctcaa ggagtcgcat ttttcctgaa ttcctgtcca aaaaaagttg
     1801 gtaagtctcg tctgggcgtt cgtactccat ttgacaaact ttacgccgaa gtgtgttccg
     1861 atggtccact gcactgtggc atcttcgatc atgacaggct cctggtcgtg agagaggtag
     1921 aggaccagcg ccagtcgcat accgatctga accggatctt ggctttcgat tcgaagccct
     1981 tctcgtgaga catcaattac ctgtcccttg cccagtatct caggggtgag atagttgatc
     2041 gaggcctgaa gagggatgcg tgggacgcat cgcccgttcc atgccttctc gtttacgacc
     2101 atatgtcccc ctggactgaa gacaattatt aattggccgt cagcacaaca taactatgcg
     2161 ccgttggagg cggtctgtca cgtataaatg tttaccgtgc acgagcaaaa cggctcaaaa
     2221 agcccgcgtc ttttgatgaa gagaaagaca tacaagcggc aattctctgg tcctccgatc
     2281 acgcgccttc cgaactgaac tcctccaggg gttggaaaaa ctgcatggtg gctcggccgg
     2341 tgcgacctcc atttcagctc gcacgccgaa tctagatagg aagctcggtc tctctattga
     2401 gcgtatccgc acagcccgcg cagttcgtga tttcacatct cgcagacacc cgcaaccagg
     2461 aatcgctcac gaatggctct attgcttgat tacacttcat cttacgatga cgttttccca
     2521 tagcctttcc gttgcgcctg gaactgaaac gtggcctcaa gtttgctcat tcgtctcacg
     2581 aacacacctg tcgtcattgg gcaggcgtcc gcgtacttgg cgttcgcaga agaaccccct
     2641 gccgacaaaa ctgaaactgt tccgatactt ttggcgatgg atagaactgc catgaccgaa
     2701 tcgcagcacc atcttaaagg cttccaattc attgtgttga acctggtgat cggtctcgct
     2761 catatggtgg tgctgttcaa tgcaggctct tatgtcgcac ttcttcccca tgcggcaggt
     2821 aatctcggcg gcgtgctgcc gagttttggc acctgggctc agacggattt catgattgct
     2881 ttggctctcg catttcccct tgctcgctgg ttatcgtccc gttttggtga gactcgtgta
     2941 ttcgttgtgt catttatcgt gtatgccatg gcgtcgggtc tgtgcttatt gggcagcgag
     3001 atcagcacat ttctgcctgc tcgtgtcctc ctaggcctcg ctggaggact gacccttccg
     3061 ctggggcagt cccttctctt gaaggaatat cctgatcgca tcaaatcgtt agggctcgct
     3121 atttggggcc tgttcacctt gatgcctttt accgcgggtc tcgctactgg aggttgggtc
     3181 gccgatgaac tgggctggcg tggactgttc gccctaaata ttccgttcgc gctgctgatt
     3241 gccagcgtgt ccaccatact cctgcctcat cacagcgtcg tctcacaggt cgaacggttt
     3301 gattttgtgg ggtttgcctt gttggcactc atcttcgccg gcatccaaac catcttcaac
     3361 ctcggcaacg atttcgattg gttcgattct tccgtgatcc aagtgaccat ggtcgtcgtt
     3421 gtcgcagcca tcccggtctg gattatttgg gaattggggg aggaacgacc agcagtgaac
     3481 atacggttgt tcctgcatcg caacttcacc gttgggttag tctctttgag cctcggcttt
     3541 ttatctatcc aagggcttct tgcgctgttt gtggtgcagc tgcaaacggt cctaggctac
     3601 tcctcgacgc ttgccggttt ggcattctta cctatgctgc tattcggcgt accactgatt
     3661 gccatcatgc atgaagtagc gaaacgcttc gacgttcgta tgctcgcttg tttcaatgcc
     3721 gtcggctttg ccgcgacgtt ttattggatt gggcttttcg atgatcctca atcctatgat
     3781 cagatcttct ggcccatggt cctcgaaggt gtcttcctcg gatcattttt cacaccgctg
     3841 acaatattga cgttacacgg gctttcaggc gaggaaatga tgagcgccgc ggaggtcgca
     3901 aatctgtttc gtcttgccgc cggtgccata gggattacgt ggcaaagcgt ggtggtgttc
     3961 cgccggattc cgttccatca attagctctt agcgatcact ttgggggtcg gatttcggca
     4021 tcctacgatg cgtggagcca aatcgtggag aagctacagg ctatcgggtt cgatcgtgca
     4081 ggaacggagc ggcatctcca gctgctcatt aagcaacacg cgggcattct ggccttgaac
     4141 gatgcctttc tcctgtccag ctgtctgtgt ctaggtgttg cagtgctagt ttggtttgca
     4201 cgaagcagcc ggatcccggc ctcgacagaa ccggatgctg tacaagcatg gcaggctgaa
     4261 gagttgatgg aacagccatg aagccacggt ggataacaag gcaggtgcta aaaaacgcat
     4321 cctgggcggt cttttggatg gtctccggtt gcgcctggat cccgaaaggc gaccctccag
     4381 cggcttacat cgaaccgcca gaactgaaag agacgctcgc cgaagtgacc agccggctgc
     4441 acaactggcc ggaggatcac tggtgggagc tctttggtaa ccctgaactg aatcaacttg
     4501 tcgaaacggc tctgaaggat aacccaggcc tcaaacgagc cgccgcgcga ctacgcgaag
     4561 cacaagggtt ggttcgcgtg gaaggagccc gccttctgcc gttcttagag gcagatgcgt
     4621 cgctgactta cgagcggatt tctcagcacg gcgtctttgc tgcattaaat ccggaggtag
     4681 ctggtgcgcg gattctcctc gggatagtga acccgctgag ttttcgttac gagttcgatt
     4741 tctggggcaa gaatcgagcg aggctggaag gtgccctcgg acaagccgcg gccgaggaag
     4801 cggaaagggc cgaagttcac ctgcgactaa gcaccggcat tgcgcgtgcc tacctccgtg
     4861 gtcaggcctt acatcggcag ttggaactcg ccgcctccat tgtggatctc cggcatcatc
     4921 ttcgccgtct cgcggaaacc cgctataagc tcggactcga taatgatctc gcagtgaaac
     4981 aagccgttgc cgactacgaa gccgcagtaa aacggcgtgc tggcgtgcgt gatcagctgg
     5041 atgtccaacg tcatttggtc gcgcgcctta ccggtaaagg gccggacgat gtccagaacc
     5101 tgttcgccaa gtcctctatc gctggactca acgaggtgcc gatccccgaa cacttgtcca
     5161 ttggactact ggtccaccgc cctgatctcg ccgcatcgct ctatcgagtg gactcggctg
     5221 ctcgacttgt gaaagccgcg aaggctgagt tttatccgac aatcgatttg acggctttcg
     5281 ttgggttcaa cgccctgacg cttacaaaag gggcagatca gctcgcaaat cttttatttt
     5341 ccggccagag cttttcctat ggcatcgcac cgggaattcg gcttccgatt tttgaaggcg
     5401 gccgtttacg tggcgagttg accgtgcatc gagctgagta tgacgcggct gtagaactgt
     5461 ataacgagac gctgttggat gccttacggg aagtggctga tagcctgagc gcctggaaag
     5521 cgaccaacga catgctcgaa tcgcatgaac gattggtcgc gtccctcaat caagactggc
     5581 atttggcaaa agtgcgtgtg gccactggtt tagatgatga ccgggaggcc ttacgccatc
     5641 gttatcccct ccttgaacag gaatacacgc ttcgcgcttt ggagagtgac cgactggttg
     5701 ccgccgtaga tctgatcgaa gcattgggcg gtggatacca taatcctgat cttgcaaagc
     5761 gaccggcgca taacgaaagc taacgatgag cacaacccat tccgtcccgc ccccagctcc
     5821 gcatctgtat gaaggatcgc tccatgcaca gcggaaccgt cgtctgttta tggtgctgat
     5881 cgtgatgatg ctcctagggc tggcgtacgc cgtgtattgg tggacaacag gtcgacattg
     5941 gattcggaca gacaacgcat atgtgacggg aaacctcgtt ccggtcacag cccaagcgac
     6001 aggcatcatt acgcaagttc ttgtcgagga aacgcagttc gttcaccgcg gcgacgtgat
     6061 aatccgactc gatgaacatg aagcctatgc ggcactggga cgggcacgtg gacggctggg
     6121 agagagcgtc cgtcgcatta acgcgttgtt cctaacgcaa aggcagctca cagaaaaatt
     6181 gagagctcga cgggcgcggt tgaacgttgt ttggcacgat gtggaccgct acaaaaaagc
     6241 ctcacccagc ggtgcagtct cgaaacaaat cctacaaaat gccctggatc aactccaagc
     6301 actcgaggcg gaggtgcggg agactcaagc tgagtacgat acgctggacg cacaaattgg
     6361 cgggacaact gtgatggaac acccggcggt cgaactggcg aagcatgaat tcattaatgc
     6421 acatcttgaa tatgctcggc agcagattct tgccccagcc tctggctacg tcgcaaagcg
     6481 caaggcgcaa gtgggtgatc gcgtcagacc tggcaccaat ctgattacca ttgtgccttt
     6541 agaccatctc tgggtggaag cgaatctccg ggaaaccgaa ctcgaacacg tccgtccggg
     6601 gcagccagcg gaaatccgtg tcgatctcta tggcgacaga catatattcc atggcacggt
     6661 ggaagggtta gtcccaggaa ccggaagtcc gttcgcccta cttccgccgg ataactctac
     6721 tggcaatttc atccatatca ccgagcgtgt gccagtccgc atcgcgttag cggcggatga
     6781 aattcgagat catcctattc ggccgggcct ctcaacattc acaaagatca gagtaaccga
     6841 gtctggtcaa tcggtatggt cgtccctagc caaggccgac accgaggagt atcagacgga
     6901 cgtgtacagc gacgagctaa ccggtgccga agctttggca ctgagtgtca tcacgggcaa
     6961 cgtgcgctcg gaccgattac ataccccgga aatggcggaa ggaacgggaa ccatattagg
     7021 ggagaaccat cgccatcgtt tttctgagcg gtcgaagcca aaatcaaacg ttccacttcg
     7081 aagtcccgac ctcggcgagg cattccttcc cttgtcgccg tcgatcgggc ccgggtctga
     7141 tcgtctcgga cctgaagccg ggccatcgac tatgcggcca cgtcgtcctg attcaacccg
     7201 tccacagtca ccctcccagt gaactcccga caacgggcgc tcctggttgg tggcaacggt
     7261 tgaaggctac accactatga ctctaaaccc tcgcgtttta agactgcctg atgaagacaa
     7321 ccgatgagat gtcggccaat gtgagtgacg attcttcaaa ggggatacga tcagtttcgc
     7381 acaccgctcc aaactgttgc ctgccaagac accagttgat gtttcctgac cttgtggtgt
     7441 aactgtccta ttggagtagc ttcagtcata agcatgatgc aatcgatggc cgagctaatg
     7501 tccgactgca atcgcctggt ttactgcgtc gtcaggcggc tcatcacgag ggagaagtga
     7561 tatgaccaaa ccagcaaaga acacaatttg tctttggtac gatagagacg ccgaggaggc
     7621 ggcgcgattt tacgccagga cgtttcccga ttc
//
LOCUS       DASTQY010000451         3754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_710714, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000451 DASTQY010000000
VERSION     DASTQY010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710714"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VFR82_14480"
     CDS             complement(<1..615)
                     /locus_tag="VFR82_14480"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4502855.1"
                     /translation="MKSLSRGKGNAEVVVVTGASAGVGRAIAREFARHGASVGLLARS
                     DDGLDGAARDVERLGGRALAITTDVSDDQQVEEAADHIERALGPIDIWVNNAMVSVLS
                     PALEMTAEEFRRVTDVTYLGYVYGTLSALRRMVPRDRGVVVQIGSALAYRAIPLQSAY
                     CAAKHAVQGFTESLRSELIYDGSHVRLTTVQLPAVNTPQFSWIRT"
     gene            complement(652..852)
                     /locus_tag="VFR82_14485"
     CDS             complement(652..852)
                     /locus_tag="VFR82_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502856.1"
                     /translation="MLTLTTGLLLLAVIVIALYVFGVFDTPYLGAFRFTFYLLLVLLA
                     LAVWYGTMNPSHEGYDPTVGAR"
     gene            complement(1019..1396)
                     /locus_tag="VFR82_14490"
     CDS             complement(1019..1396)
                     /locus_tag="VFR82_14490"
                     /inference="COORDINATES: protein motif:HMM:NF018767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HEU4502857.1"
                     /translation="MNAIGKKRWAIAEGYIPPTSTGPHPQMTSHETVCLLNTGPEDAH
                     VSITIYYVDQPPVGPYRIVVPSERTRHVRFNDLLDPAPIPSDRDFASLIESDVPIVVQ
                     HTRLDSRQAENALLSTIAFAGNE"
     gene            1638..1844
                     /locus_tag="VFR82_14495"
     CDS             1638..1844
                     /locus_tag="VFR82_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502858.1"
                     /translation="MRLINRKAGVVIGLTVILTFPVTVVSGAGSQKKGEIETRCIVAS
                     HKVPVERTGVIGDKGGPSMTQVPR"
     gene            2060..2743
                     /locus_tag="VFR82_14500"
     CDS             2060..2743
                     /locus_tag="VFR82_14500"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEU4502859.1"
                     /translation="MKTTMTVIIMALFTCFLVANGPVSAEPTGSSTIAGGSTPSPEKI
                     PQDKRTDLTGGQQGVPDEYSTTPVRQGKLKEVNDSKLLNQTVTNPQGEKIGKITKVLK
                     DETTQNIEYVMFQLQDAQSARPLPWSKFQEKGDKIVLNATKEELRPSVSSKDAKDMSP
                     DLALFMEEIEQKRNEPKASGGGNERPSGDPGSLSGAATMGEDKVGSHRSAPPTPAPQF
                     ENDAQKGKK"
     gene            3134..3655
                     /locus_tag="VFR82_14505"
     CDS             3134..3655
                     /locus_tag="VFR82_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502860.1"
                     /translation="MNARQIMLGSALLMLAGCQSIGGEGMPTLTRTGEVKDVIIRDVV
                     EPTSVTVNPGDEVRWINKRQGDVRVIFLNPMTENLACQRNFGGMMGFGTKRNEYSAKL
                     SPNDTASVCFRDAGEVKYVVRATSGDPIGEQNIAGTIQIGGDRAAAREFSREKQEHRS
                     AQQQPEDETTRAR"
BASE COUNT          893 a          993 c         1107 g          761 t
ORIGIN      
        1 cgtgcgaatc catgaaaatt gcggagtatt cacggcaggc agttgcaccg tggtcagccg
       61 cacgtggctc ccgtcataaa tgagttcaga tcgaagcgac tccgtgaaac cttgtacggc
      121 atgtttcgcc gcacagtaag cagactgcag cggaatagcg cggtaggcca gcgcagagcc
      181 gatttggacg acgacgccac gatcgcgtgg caccatgcgc cgcagcgccg acagggtccc
      241 atagacatag cccaggtagg tgacgtctgt gacgcggcga aattcctccg ccgtcatctc
      301 gagggccgga gacaggaccg acaccatcgc attattgacc cagatgtcga tcgggcctaa
      361 ggcgcgttca atgtggtcgg ccgcctcttc gacttgctgg tcgtccgaga catcggtggt
      421 gatggctaaa gcgcggccgc ccagccgctc gacatctcgt gcggcgccat ccaatccgtc
      481 atcgctacgc gccagcaggc cgacactggc tccatgacga gcaaactcgc gggcaatggc
      541 tcgtccgacg ccggccgacg ccccggtgac gacaacgact tcagcattcc cttttccgcg
      601 actaagtgat ttcacagtca tcctccattt atcgcatctt gaccctgcgt atcatcttgc
      661 tccaaccgtc ggatcgtatc cttcgtgaga tgggttcatg gtgccgtacc aaacagcaag
      721 cgccaacaag actaacagta ggtaaaaggt gaaacgaaat gcccctaaat acggtgtgtc
      781 gaatacgccg aatacataca gcgcaatgac aatgactgcc agtaataata ggccggtggt
      841 gagtgtcaac atggaagcct cctgctcagc cagtctcgtt gccggcgttg ctgcgtcaag
      901 accaagatcg gatgtcaggg cgatgtagaa ccagcacgat gggttgtagg gccatacaat
      961 ggggatatcg gaattggttc cgtcgtttga atggctcgag gtcacgtagg cgttcgcatc
     1021 attcatttcc cgcgaaggcg atcgtgctga gaagcgcatt ctccgcctgg cgtgaatcga
     1081 gccttgtgtg ttggaccacg atgggcacgt ccgattcgat cagactcgca aaatctctat
     1141 ccgacgggat gggcgcagga tccaataaat cgttgaaccg cacgtgccgt gtccgctcgg
     1201 acggcacgac gatgcggtag ggtcccaccg gcggctgatc gacgtagtaa atcgtgattg
     1261 acacatgcgc gtcctctgga ccggtgttca acaagcacac cgtttcatgg ctcgtcatct
     1321 gcggatgcgg tcctgtgctc gtcggaggga tatacccctc ggcaatcgcc catcgctttt
     1381 tccctattgc attcatcaaa tagtagagct gcaagccaag tgcctatcag gactgacccc
     1441 cggttaacgg gttggagagc agacctgcgg gaggtggaac ttctgtccgg tggtagacaa
     1501 gttgtctgag aatctggagc ctgcggtcaa cataacgcta gggaaacccc cgtctttctg
     1561 atgcggccta tgacttgcac tactcacgtg cgatgggcat tcacatccat catccaggcc
     1621 ggcgtttctg aacggggatg aggctgatca atcgtaaagc cggtgtcgtc atcgggttga
     1681 ccgtcattct tacctttccc gtgaccgtgg tatccggagc cgggtcacaa aagaagggag
     1741 aaattgaaac ccgatgcatc gtggcgagcc acaaagtacc agtggagagg acgggggtga
     1801 tcggggataa gggcggccca tcaatgaccc aagttccccg ctgacatact cgtggggcgc
     1861 tgtgatagag cgcacagcta ataggtggac tatgcaacag agtgccagca gcacgcatga
     1921 atgtttgaag agtgagttga tgtctgccgc ggatcagggg ggccgctggg aacggcaggg
     1981 ctcagggctg accgaatggg tgaacgaagt ggcgagtgaa tgggttcaac ggcatttctc
     2041 taataacagg agggctttca tgaagaccac aatgaccgtg attatcatgg ccttgttcac
     2101 gtgtttcctc gtagcgaacg gtcccgtttc ggcggagccg accgggtcct cgaccattgc
     2161 aggtggcagt acgccatcac cggaaaagat cccacaggac aaacggacag atcttactgg
     2221 cggccagcaa ggcgttccgg atgagtattc cacgacgccg gtgcgtcaag ggaagctgaa
     2281 ggaagtcaac gacagcaaat tactcaatca gactgtcacg aatccgcaag gggaaaagat
     2341 cggcaagatc accaaggtct tgaaggacga aacaacgcag aatatcgagt acgtcatgtt
     2401 ccaactacag gacgcgcaaa gcgcccgacc acttccttgg agcaaattcc aggagaaggg
     2461 ggacaagatc gtgttgaatg cgacgaagga agagctgcgt ccgagcgtca gttcgaagga
     2521 tgccaaggac atgtcccccg acctagcatt gttcatggaa gagattgaac agaagagaaa
     2581 cgagcctaag gcgtctggcg gcgggaacga gcgcccgagt ggcgatcctg gttcactctc
     2641 tggtgcagcg acaatgggcg aggataaagt cggaagtcat cgcagcgcgc ctccgactcc
     2701 ggcgcctcag tttgaaaatg atgcgcagaa aggcaagaag taacaggccg tattctgcgt
     2761 caggtcaggg tcacgcccga tcggtccgag tatttggatc gatcgtggcg tgccggacct
     2821 ccaccccgtt gctgatccct ccgccataaa gtccatcctg ctcggcgacg gggacgactg
     2881 caagctgtcc gtcctcgaat cgtatacccg gaaagcgaca aagccgcagg aatgcttgtc
     2941 cgatggcagg cgtaatttgt ctaaagtttt gacggactga aatagacgcc gtacaaacgc
     3001 ggcagcgctg ggagatgtcc gggttttact cggcctgtct cttgcagtgc tctcctttta
     3061 cctgatctgc aggcatgaac ggctaagaac acggcaaact tttcacacat gatctactga
     3121 aggaggaaag acaatgaatg cacgacagat aatgttgggt tccgctctgc tcatgctggc
     3181 cggttgtcaa tcgatcgggg gcgaaggcat gcccacgtta acccgcaccg gcgaagttaa
     3241 ggacgtcatt atccgtgatg tcgtcgagcc gacatcagtg acggtcaatc ccggcgatga
     3301 ggttcgctgg atcaacaagc gtcaaggcga tgtacgggtc atattcttga atcctatgac
     3361 ggagaacctc gcttgccagc gaaattttgg tggcatgatg ggatttggga caaagcgcaa
     3421 cgagtattcg gccaagttga gtcctaatga cacggcaagc gtgtgtttcc gcgatgccgg
     3481 cgaggtgaaa tatgtggtga gggcgacgtc gggcgatccc atcggcgaac agaacattgc
     3541 tggcacgatc cagatcggag gagatcgtgc tgctgctcgt gaattcagcc gagagaaaca
     3601 ggagcatcgt tctgcccaac aacagcccga agatgaaacg acgagggccc gctaggggaa
     3661 acacgggtcc gactactggg gtttgagcgg ttcgtcagct agagaaatta ctaatgccct
     3721 tcatctctag cgtatgtttt gatgggcaac gtcg
//
LOCUS       DASTQY010000452         6397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_512385, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000452 DASTQY010000000
VERSION     DASTQY010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512385"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VFR82_14510"
     CDS             <1..407
                     /locus_tag="VFR82_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU4502861.1"
                     /translation="SAVPFALIGGVIALVATHTNFSISAAVGIISTLGVAILGGVLLI
                     SRIEDGRMAGLNLREAIIQAAAVQMRPILMATIGAAIGLLPAAVATGIGSQAQKPLAR
                     VVVGGMLTAAFLILVVLPVLYEMVHRRTDHDD"
     gene            423..1220
                     /locus_tag="VFR82_14515"
     CDS             423..1220
                     /locus_tag="VFR82_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU4502862.1"
                     /translation="MYTRLKWMWVTVVVMGLGVGIGGPELFAQSGHRGLTVPVQIPYE
                     MPPPFQETSPAVSVPPNAKPLTPEELQRAESLLPMLEGKQEFWAMGEFVHLGEPSVPV
                     LVKALTMPGPRTRYNAIETLSMLNAASAVPALIASAKNPNEIPRVREHALRVAIRLDA
                     SKTPEAIQSMANDQNSSIRKAAAFGSRYVRDKAVVPVLIGMIADDERFVALSAVQSLW
                     ILTLHETEFHDWEASTKADRQEWMQEWTEWWSGQKETFQIPEPRRRS"
     gene            1337..1867
                     /gene="def"
                     /locus_tag="VFR82_14520"
     CDS             1337..1867
                     /gene="def"
                     /locus_tag="VFR82_14520"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU4502863.1"
                     /translation="MAVLPIAKLGNPVLRKIAAPVDPREIRTREFQQLIDDMYETMLE
                     EPGIGLAAPQVSRSIQLVVMACEGEGGFPTTTLINPTIVYYGPQQVENWEGCLSLDGL
                     RGKVTRPALVRVKALDRNGKPLDIEADGLYSVCIQHEMDHLIGKVFVDRMTDLSTLTQ
                     LNEFDKFWRKEPTPVI"
     gene            1889..3613
                     /locus_tag="VFR82_14525"
     CDS             1889..3613
                     /locus_tag="VFR82_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4502864.1"
                     /translation="MKTLLRVLQYLRPHRPMVIGTFLFAGLTTAFELVPPWLIKVLID
                     DVIQAGKTQLLSLVFVGLCAAYVLRSVCASMRIKLNNSLEQQIVHDLHVQVFGALQRL
                     SISFFENRPTGEIMSRVLNDTEHMQRIFVDGLEEIITAGLTLIGIMAVLFWLNWKLAM
                     LALVPIPLLVLGAIAFTKRIHKYYRKIRKGSAELNALLQDTLAGIRETMGFNRQPYEE
                     DRFGRKSDQCRKDTLKAMYLWSYYSPGMMLAGSLGAALVLWNGTHEVLEQHLSVGELV
                     MFMSYLALFYVPINQIHSVNHMLQHALAASERVFEVLDIVPEVKDEPGLRIPAPRLTG
                     EIAFRSVHFHYRPDAPVLYDVSLIVRAGERVALVGPSGAGKSTTLKLLTRFYDVTGGE
                     ITIDGHDLREMPLAFLRSQIGLVQQEPFLFNGTVRENILYGNLSASHDEVEAAATAAR
                     AHEFIVNLPEGYDTWIGERGVKLSVGQRQRVSIARVLLKDPPIVMFDEATSNIDTETE
                     VKIREALEELTKDRTTIVIAHRLSTVHGMDRIIVLEHGRVVEEGPHELLITRGGVYAG
                     LYEAQFQI"
     gene            3858..4067
                     /locus_tag="VFR82_14530"
     CDS             3858..4067
                     /locus_tag="VFR82_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502865.1"
                     /translation="MARSAGRKTVAVMRGLMMLCDSCYDQVLAEHLTDHKNFVQNYEA
                     LFDTICPGCTDINRPLVDDMLGSSE"
     gene            complement(4265..4588)
                     /locus_tag="VFR82_14535"
     CDS             complement(4265..4588)
                     /locus_tag="VFR82_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502866.1"
                     /translation="MAYMRTILLAFVVLLLARPGLAEDKSYYSPVIQVDKDNHRILIS
                     TLGSVFYIDVPAAAQPHMEKLPISGLVDFVVEMRGDGQMPLVKTWKVKSGESTCMYFN
                     GKECK"
     gene            complement(4693..5205)
                     /locus_tag="VFR82_14540"
     CDS             complement(4693..5205)
                     /locus_tag="VFR82_14540"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEU4502867.1"
                     /translation="MSDLTIPRQVINDLIAHARELDPYECCGFLAGTDKTVTQLYRIK
                     NVVSLDGASEAASFDEAKLNHLSRLSPEERAEVAFIMDAQEMFQAVKDMRKNGLTLQV
                     VYHSHPHDPARPSVTDIKIANEWEENWSRLNLTLPIYMLISLMDKARPDVRAYWIKNG
                     SVSSADVVTL"
     gene            complement(5207..6010)
                     /gene="moeB"
                     /locus_tag="VFR82_14545"
     CDS             complement(5207..6010)
                     /gene="moeB"
                     /locus_tag="VFR82_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEU4502868.1"
                     /translation="MELTEQQIERYSRHIILQDVGGKGQLKLSKAKVLLIGAGGLGSP
                     AGLYLAAAGIGTIGLVDGDVVDLSNLQRQILHSTATVGRPKVESGKQTLAAINPEITI
                     HAYHQLVDADNILSLVKQYDVVLDGSDNFTTRFLVNDACFFAKTTLVSASMFRFEGQL
                     TTIKPHAGYPCYRCLYPEPPPAGLVPNCQEAGVLGVLAGTMGILQASEAIKEILGIGE
                     TLADKLLIYDALEMKFRKVSRPKDPGCPLCGPNPRIKDLSTDYTVSCSI"
     gene            complement(6052..6291)
                     /locus_tag="VFR82_14550"
     CDS             complement(6052..6291)
                     /locus_tag="VFR82_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HEU4502869.1"
                     /translation="MAHLRFHVRFPEDKVKEPIIYQIGREFNVVTNVRRADVRETTGW
                     VDVELSGETAEVERAIQGLRHKGCVVDPIELNVVE"
     gene            complement(6291..6395)
                     /locus_tag="VFR82_14555"
     CDS             complement(6291..6395)
                     /locus_tag="VFR82_14555"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU4502870.1"
                     /translation="MYVNEEDIRFLKGKDTSLTAGDEVSIVPAIAGGR"
BASE COUNT         1392 a         1721 c         1849 g         1435 t
ORIGIN      
        1 tttcggcggt gcccttcgcg ttgatcggag gcgtgatcgc gctggtcgcc actcatacga
       61 actttagcat ttcggccgcg gtaggtatca tttccacctt gggggtagcg atcctgggcg
      121 gcgtcttact aatctcccgc atagaagacg gccgtatggc cggactgaat ctccgcgaag
      181 cgatcatcca ggcggctgcc gtacaaatga gaccgatttt gatggcgacg atcggcgcgg
      241 cgatcggact gctgcccgca gccgtggcga cggggatcgg ctctcaggcc cagaagcctc
      301 tggccagggt ggtcgtcggt gggatgctca cagccgcctt cttgattctg gtggtgttgc
      361 cggtgttata tgaaatggta catcgccgaa cggaccacga cgattaacga aagaggatga
      421 tcgtgtacac gagattgaaa tggatgtggg tcacagtcgt cgttatgggt ttgggcgtag
      481 gtattggcgg acccgagctg tttgcacaat caggccatcg cggcctgacc gttcccgtgc
      541 aaattcccta tgaaatgccg cccccgtttc aggagacttc ccctgccgtt agcgtccctc
      601 ccaatgccaa acccctgact ccggaggaac tccaacgcgc ggaatccctg ctgccgatgc
      661 tggaggggaa gcaagaattc tgggccatgg gggagttcgt ccacctaggg gagccgagtg
      721 tacccgtcct tgtcaaagcc ttgacaatgc cggggccgag gactcgctac aatgcaattg
      781 agacgttatc gatgctgaat gcggcctcgg ccgtccccgc gttgattgcc tccgcaaaaa
      841 acccgaacga aattccccgc gtaagagagc atgcgctgcg tgtggcgatc cgtctggatg
      901 cctcgaagac gccggaggct atccagtcga tggccaacga tcaaaattct tcgatcagaa
      961 aggcagcggc tttcggctcg cgttatgtcc gagacaaagc ggtcgtaccg gtgctcatcg
     1021 ggatgatcgc ggatgatgaa cgctttgtcg ccctgtccgc cgtccaatcg ctctggatcc
     1081 tgacgctgca tgagaccgaa ttccacgatt gggaggcgtc cacgaaagcc gatcgccagg
     1141 aatggatgca ggagtggacg gagtggtggt caggacagaa ggagaccttt cagattccag
     1201 aacctcggcg gcgctcgtaa tagacgccgt aggtgccgtc tgctgtaagt atgtttcaac
     1261 atgttgcggc tcaaaaaaac ggtgtgttat acgtcatgca aggcattaac caagaggctg
     1321 tggcgaggca aatgtaatgg ctgtcctccc gatcgcaaaa ctgggaaatc ctgtcctgcg
     1381 taagattgcg gctcccgtgg acccgcgcga gatacgcacc agggaattcc agcagctcat
     1441 cgatgacatg tatgagacca tgctggaaga gccgggcatc ggtttggcgg cgccgcaagt
     1501 ctcgaggtcc atccaactgg tcgtcatggc ctgcgaaggc gagggcgggt ttcccacgac
     1561 cacgctgatt aatccgacga ttgtgtatta tggtccgcag caggttgaaa actgggaggg
     1621 ttgtttgagc ctggacggct tacgcgggaa agtgaccaga ccggccctcg tgcgagtgaa
     1681 ggcgttggac agaaacggca agccactcga cattgaagcc gacggactct attcggtttg
     1741 tatccaacac gagatggacc atttgatcgg gaaagtcttt gttgaccgga tgacggatct
     1801 gtccacgctt acccagttga atgagttcga caagttctgg cggaaagagc ctacccctgt
     1861 catctgagcc atccttcgat cgatcgctgt gaaaacgcta ttgcgagtcc tccagtatct
     1921 tcgcccgcat cgcccgatgg tgatcggcac ctttctgttt gcaggactga cgacggcgtt
     1981 cgagctggtc ccgccctggc tgattaaggt gctcatcgac gatgtcattc aggccggtaa
     2041 gactcagctc ctgtcgctgg tcttcgtcgg actctgcgcc gcctacgtgc tgcgtagtgt
     2101 gtgtgcctca atgagaatca agctgaacaa cagcctggag cagcagatcg ttcacgacct
     2161 gcatgtgcag gtcttcggcg ccttgcagcg tctgtcgatc agttttttcg agaaccgccc
     2221 cacgggcgag atcatgtcgc gagtgctcaa cgatacggag cacatgcaac ggattttcgt
     2281 tgacggcctg gaagagatca tcactgcggg cttgacgttg atcggtatca tggccgttct
     2341 cttttggctc aactggaagt tggcaatgct cgcgttggtg ccgattcctc ttcttgtgct
     2401 cggagcaatc gcctttacga aacgaataca taagtattac cggaaaatac gaaaaggatc
     2461 agcggagttg aacgctctgc tgcaagatac gctcgcgggg attcgcgaaa cgatggggtt
     2521 caatcggcag ccctatgaag aggaccgttt cggccgtaag agcgaccaat gccgcaagga
     2581 tacgctcaaa gcgatgtacc tctggtccta ttactcaccg ggtatgatgc tcgccggcag
     2641 tcttggagcg gcgcttgtcc tgtggaacgg cacgcacgag gtcctcgaac aacatctctc
     2701 cgtcggcgag ttggtgatgt tcatgtccta cctcgcctta ttctatgtgc ccatcaatca
     2761 gattcattcg gtcaatcaca tgttgcagca tgcccttgca gcaagcgaac gggtgtttga
     2821 agtcctggac atcgttcctg aagtgaagga tgagccgggg ctccggattc cggcgcctcg
     2881 gctgaccggt gaaatcgcct ttcgcagcgt acactttcat tatcgcccag atgcacccgt
     2941 cctatacgac gtgtcgctca tcgtgcgagc cggcgagaga gtggcgctcg ttggtccaag
     3001 cggtgctggg aagagtacga ctctaaagct gctgacccgt ttctacgatg tcacgggagg
     3061 ggagatcacc atcgacgggc acgaccttcg agagatgccg ctcgcttttc tccgcagcca
     3121 gatcggcctt gtccaacagg aaccgttcct cttcaacggc acggttcggg agaatatttt
     3181 gtacggcaac ctctcggcga gtcatgacga ggtggaagcg gctgccacag cggcacgtgc
     3241 ccacgaattc atcgtcaatc tgcctgaagg atacgatacc tggatcgggg agcgcggggt
     3301 gaagttgtca gtcggtcagc gacagcgcgt gtcgattgcc cgggtgctct taaaagatcc
     3361 tccgattgtg atgttcgatg aagcgacgtc caacatcgac accgagacgg aagtaaagat
     3421 tcgagaagcg ctggaggagc tcaccaaaga tcggacaacg attgtcatcg cccatagact
     3481 ttcgaccgtt cacgggatgg accggatcat cgtgctggag catggccgtg ttgtcgagga
     3541 gggtccccat gagctgctta tcacccgtgg cggtgtctat gcgggacttt atgaggccca
     3601 gttccagatt taggaagatc ttatgtcccg tcacagacta cgcctgacgg cattcttatg
     3661 gtcttgatct acgaaaacga tccgctggat aatgaagatg ctcgcggacg gttctatcat
     3721 gtctgtcgag ggcggatcgt gcgcacggag atccaaatac catcggatgc gacagtgtac
     3781 gaatgcgctg tctgcgggat tgacctggag ccagaagatt tctggctcgc ccgacagaaa
     3841 ggatcggtgt agacgagatg gctcgcagtg caggcagaaa aaccgttgcg gtcatgcggg
     3901 gactgatgat gttgtgtgac agttgctatg accaagtcct cgcagaacac ctgaccgacc
     3961 acaagaattt cgtgcaaaac tacgaggccc tgttcgacac catttgtccg ggatgtaccg
     4021 acatcaatcg tccgttggtc gacgacatgc tgggcagctc ggaatagggt gactgcgagg
     4081 gtgagggtcg accgaccgct acatcagaat gaaaggtcgt cctgcaaggc tgcagcgagc
     4141 gaaaatctga gacgcactcc gttcggtacg ttgaaggttt gagcgacgcg agaacgcagc
     4201 ctggtgaaga cgtttgctaa gcgctggggt ggcccgggct agaaaacctt tctcagcttc
     4261 ctgtctattt acactccttc ccgttgaagt acatgcaggt cgattctcct gatttcactt
     4321 tccacgtttt cacgagcggc atctgaccat cgcctcgcat ttcaacgacg aagtccacta
     4381 aacccgaaat cggcagcttc tccatgtgcg gctgagcggc agccgggacg tcgatgtaaa
     4441 acacggaacc gagtgtggaa atcagaatgc gatggttgtc tttgtcgacc tggatgacgg
     4501 gactgtaata actcttgtcc tctgccagtc ccgggcgggc aagtagcagc actacgaaag
     4561 cgagaagaat cgtacgcatg taggccatga cgatcactcc ttctcaaaaa gggtcgaaga
     4621 tggcgtcatt atacgcaccg gtctaaaaag aaggaaactg ctatttcgaa ccggcgggtg
     4681 agagggatca agttacaggg tgacgacgtc ggcggaagag accgagccgt ttttgatcca
     4741 gtaagcccgc acgtctggac gagccttgtc catcaacgaa atgagcatgt agatcggcag
     4801 ggtcaaatta agccggctcc agttctcttc ccactcgttc gcaattttga tgtcggtgac
     4861 cgatggtctt gccggatcgt gagggtgcga atgatagact acttgtagtg tcaacccgtt
     4921 ttttctcatg tctttcaccg cctgaaacat ctcttgggca tccatgatga acgcaacctc
     4981 cgcccgctct tccggcgata gccgtgaaag atggttcagc ttggcctcat cgaaggaagc
     5041 cgcttcggaa gcgccgtcca gactcaccac atttttgatc cgatagagct gcgtcacagt
     5101 tttgtctgtg ccggccaaaa atccacagca ctcgtacgga tcgagctcac gcgcgtgagc
     5161 gataaggtcg ttgataacct gtcgaggaat ggtcagatcg gacacgttat atcgagcagc
     5221 tgacggtgta atcggtgctc agatccttga tgcgaggatt tgggccgcag agaggacacc
     5281 ccgggtcttt cgggcgactg accttcctga acttcatctc cagggcgtcg tagatgagca
     5341 gcttgtcagc gagcgtctcg ccgatgccga gtatttcctt gatcgcttcg gacgcctgta
     5401 agattcccat tgtcccggca agcacaccga gcacgccggc ttcctggcaa ttgggaacca
     5461 gacctgcagg cgggggttcg ggatagaggc agcgataaca gggatagccg gcatgaggct
     5521 tgatcgtggt caattggcct tcgaagcgga acatgctggc tgagaccaat gtggttttag
     5581 caaagaagca ggcatcatta accaaaaacc gtgtcgtaaa attgtccgag ccgtccagga
     5641 ccacgtcata ttgcttgacc aacgagagga tgttgtcggc atccaccagt tggtgatacg
     5701 catgaatggt gatctccgga ttgatcgccg caagcgtttg tttgcctgac tccaccttgg
     5761 gccgtccgac cgtcgccgta gaatggagga tctgccgttg caggttggag aggtcaacca
     5821 catcgccgtc gaccagtcca atcgtgccga tgccggccgc cgccaaatac aatccggcag
     5881 gcgatcccag ccctccggct ccgatgagga gcactttggc cttgctgagt ttcaactggc
     5941 ccttgccgcc gacgtcctgc aggatgatgt gacggctgta tcgctcgatc tgttgctcag
     6001 taagttccat gcctcaattc accggttacg ggggcgcacg ccaggcgtga gctattcgac
     6061 cacgttcaat tcgatcggat caacgacgca gcctttgtga cggagtccct ggatcgcgcg
     6121 ctcgacttcc gctgtctctc ccgacaactc gacatcgacc catcccgttg tttctcgcac
     6181 atcggcccgg cgcacgttgg tcacgacgtt aaattcacgt ccgatctgat aaataatcgg
     6241 ctctttgacc ttgtcttccg gaaagcggac atggaagcgg aggtgtgcca ttatcgacct
     6301 cctgcaatgg cgggaacaat tgacacttcg tcgccggctg tgagcgatgt gtcttttccc
     6361 ttcaaaaagc gaatgtcttc ctcattgaca taaatat
//
LOCUS       DASTQY010000453         7950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_927248, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000453 DASTQY010000000
VERSION     DASTQY010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7950
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927248"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VFR82_14560"
     rRNA            complement(<1..420)
                     /locus_tag="VFR82_14560"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(835..1710)
                     /locus_tag="VFR82_14565"
     CDS             complement(835..1710)
                     /locus_tag="VFR82_14565"
                     /inference="COORDINATES: protein motif:HMM:NF014515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HEU4502871.1"
                     /translation="MSPNADHALSIFDVCRIPIMTRVRCAKHLVILVMVCTLLAVSDP
                     VAFGQLSAQQEPRASERTTANPETPPSALGMQSEQVIVRPTEGYFAGFGGYTFGGKFD
                     AEGTGAFSGVSFGNGSLANSAVVGAKAGGFFPEALNWFGVEAELFNTTPHIEQQGAAA
                     GSHMRVTTLAINAIARAQFACEKKHVETEQVTERFAFHYVTEFCRLQPYAGVGLGIYW
                     VDISNSAFSAHANFVPGFNALAGVRYYVTDRIALFTEYKYNRATFNFAGVQGLVGFEG
                     VYSVNHIVGGISYHY"
     gene            complement(1694..3229)
                     /locus_tag="VFR82_14570"
     CDS             complement(1694..3229)
                     /locus_tag="VFR82_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEU4502872.1"
                     /translation="MGTYSWQSASLAGVGMLVFMVSACKMGPDYVRPETPKADSWRVP
                     TSTAESIANLAWWDLLKDKELQELIRIALTENQDVRTAVASVEQYRAQLVMTRWDLAP
                     ALSYGGTAFLYQTQGGNATTIPGGGGAIVLPGTTGGDNTTFSNTIGFGSLKWEIDLWG
                     RLRRAVESSQGQLLAQEENQRAVILSLVGSVSEAYFSLRSLDLQVDITKRTLKTWEES
                     VRLSQLKFKQGYIPKLDLDRFEAERAGTAAKLAELEKQVVQTENHLSVLLGRRPASIT
                     RGLKLTEQPIPPEVPAGLPSELLQRRPDLLQAEHTLASATAGIGVAQANRFPQFALGG
                     SLAGANANLNGNQLGPLFIQNISANLAGPLLNATALGYQVTVNETKAQQAALQYEKAI
                     VNALREVEDALISVQKTREQREAQEQQVAALQSAFHFAEQRYQGGRASYLDVLTSQRS
                     LFESELALARTLQGQLVSIVQLYSALGGGWSVTDHPENNLPDPRSINSRQPSAVLVHE
                     PQR"
     gene            complement(3241..6486)
                     /locus_tag="VFR82_14575"
     CDS             complement(3241..6486)
                     /locus_tag="VFR82_14575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEU4502873.1"
                     /translation="MAKFFINRPIVAMVISIIMVILGVIAMVQLPIAMFPNIAPPEIL
                     LQATYVGADAVTLEKSVATPVEQLMSGVDNMLYMYSVNANNGQMSLRVNFDINTRPND
                     DQILTQMRYTQAESQLPADVKNFGVTIKKATTSPLALFSLYSPNGTYDATFLANYGYV
                     NINDPMTRVPGVGQVQIFGAGQYAMRFWVRPDHLAKLGITVTEITNAIKAQNTVNPAG
                     QVGAEPVPPGQEFTYSVRSQGRLESEEEFGNIVVRANQDGSMVRLKDVARIELGAQMY
                     NIVGRMNGKPAALIAIYQLPGSNAIQTMDAATKLMEEMKAQFPPDLDYVTALDTTQAV
                     REGINEIVHTLFEALILVIIVVYIFLQGWRATLIPLLAVPVSLIGTFTLFPLMGFSIN
                     TLSLFGLVLAIGLVVDDAIVVVEAVEHHIEHGMSPKEASLKAMEEVSGPVIAIALILA
                     AVFVPTAFIPGITGRLYQQFAVTIALSVVISAFNALTLSPALSALLLRPRKPARGPLG
                     LFFGWFNKWFGRATDGYVSVCGHLIRKAGRSMVFLLLVTIVAVWLGSQLPSGFLPMED
                     QGYVYLNVQLPEASSLQRTDEVCRKIEAILKSTPGIEYVSTIVGFSLLSTVSTTYNAF
                     FFVTLAPWEERKKSEEQLPAIFKNVNSRLAELPEAKAFLFPPPAIPGVGTSGGVSFVL
                     EDRMGRDIEFLADHTQKFMEAARKRPELARVDTTFIPAVPQVFARVDRDKVLKQGVNL
                     SDVYQTLQSFMGGVMVNFFNRFGRVWQVYVQAEGEFRTQAENVGQFYVRNANGDMVPL
                     SALVTMDIFHGPEFTMRYNEYRSAQLIAGAAPGYSSGQAMKAVEEVFASTMPGGMGLD
                     YMGMSFQEKAAAEGVPATVIFGFSLLFVFLILAAQYESWALPFSVLLGTPIAVFGAFA
                     ALWLLKLVAPQASENDVFTQIGLVMLIGLAAKNAILIVEFAKVEFEKGKSIAEAALAG
                     ARLRLRPILMTAFAFILGVLPLVLATGAGAHGRVLLGLTVFGGMLAASVIAIFLVPVT
                     FYVVESLVHRGTPHSEVPPLPPSQKAGDGEPSRQTPRPALSPTHAREGESY"
     gene            complement(6533..7711)
                     /locus_tag="VFR82_14580"
     CDS             complement(6533..7711)
                     /locus_tag="VFR82_14580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU4502874.1"
                     /translation="MDHAHRGRLLARSAALVLVSLSPAAFVHCKGETASAPPPPSVQV
                     EVATVIQKDVPIQMEWVGTTDGSNNALIRAQVGGYLMKRPYTEGSFVKKGDLLFELDP
                     SKFRTALDQAMGDLAKAQAQFTKTKQDVERDTPLAKEGAISQKELDDSIQAHAATKGM
                     VAAAKAAVEQAKLNLSWTRITAPIDGVVGVSKAQIGDLIDASTELTSMSTLDPIRVYF
                     PVSEQEYFEAADKVQQGYKDRKEGKEYSGALLELVLGGEKIYPHKGQFYLVDRQVDTK
                     TGTIRVAALFPNPNNLLRPGQFARVRAVTKTKEGAILVPQRAVTEMQGSYQVAVVSPD
                     NKVDIRPVKVGQRSGSLWIIDHGLKAGERVVVEGLQKVKAGMTVDPKPFQDQQEEKLG
                     "
     gene            complement(7798..>7950)
                     /locus_tag="VFR82_14585"
     CDS             complement(7798..>7950)
                     /locus_tag="VFR82_14585"
                     /inference="COORDINATES: protein motif:HMM:NF014486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HEU4502875.1"
                     /translation="GDARPDREWHIKHFRDPQSVSPGSIMPKFPLTEGELQDLTSYML
                     SLKRTT"
BASE COUNT         1711 a         2367 c         2128 g         1744 t
ORIGIN      
        1 ttcatccccc acgcggcgtc gctgcgtcag gctttcgccc attgcgcaat attccttact
       61 gctgcctccc gtaggagtct ggcccgtgtc gcagtgccag tgtggctgat catcctctca
      121 gatcagctac ccgtcgatgc cttggtgagc cgttacctca ccaacaagct gatagggcat
      181 gagcccatcc aagagcgcga tacccacggt atcgctttcc ttccgaaacc gcagttccgg
      241 aagcgtacgc ggtattagcc aacctttcgg ctggttatcc cccactcgag ggtaggttac
      301 ccatgtagtc ctcacccgtt cgccactgta catgtgtatt gctacacatt ctcgttcgac
      361 ttgcatgtct taggcgcgcc gccagcgttc gttctgagcc aggatcaaac tctcatatag
      421 gtgttgctta aatttggacc aagggccacc acttcaatac accttacacc ttgctcaacg
      481 tcctctattc agttgtcaaa gagcgatcgc gttcatcacg cgagccgcgc actatacagc
      541 gcacggcata ccgtgtcaag ggacatgctc ggagctaacg cttcgggtag tttcccctta
      601 gaaccgcacc gtgacgcgcc gtcagacggt ggtcaacagg caggatcact gaaacggaac
      661 taaccttata tcaatcggat cgaacggagt caagagggtt cgcgaccagt cccgttcgcc
      721 ccgcgcacca taatccgcac gaactcccag tcaaaccccg ctgacaattg atgataatcc
      781 ttctaaacga gtcaacgctc gggctcaacg ctcggcggcg cagttgtggc caccttaata
      841 atggtaagaa atccctccca cgatatgatt cacgctgtat acgccctcga acccaacgag
      901 tccttggact ccggcgaaat tgaacgttgc ccggttgtac ttgtactccg tgaacagggc
      961 aatccgatcg gtgacgtaat acctgacgcc ggccaaggca ttgaatcccg ggacaaagtt
     1021 cgcatgagcc gagaaggcgc tatttgagat atccacccaa tagataccca aaccgacccc
     1081 tgcatacggc tgcagccgac aaaattccgt gacatagtgg aaggcaaatc gttccgtgac
     1141 ctgttctgtt tctacatgtt tcttctcgca ggcaaactgc gctcgggcga ttgcattgat
     1201 ggccaacgtc gtcacgcgca tatggctgcc ggctgccgct ccctgctgct cgatgtgggg
     1261 tgtcgtgtta aacaattcgg cttccacgcc aaaccagttc agcgcctcag ggaaaaaccc
     1321 gccagctttc gcaccgacca cagcagaatt agccaggctg ccgttgccga aactgacccc
     1381 gctgaatgcc cccgtgccct ccgcgtcaaa tttcccaccg aacgtgtagc cgccaaatcc
     1441 agcaaagtag ccttctgtgg gccgcacaat gacctgttcg gattgcatgc cgagcgccga
     1501 aggtggtgtc tccggattcg ccgtcgtgcg ttcggacgct cgaggttctt gttgtgcaga
     1561 aagttgacca aaggccacgg ggtcgctgac ggcgagcagc gtgcacacca tcacgagtat
     1621 gacgagatgt tttgcgcaac gcactctcgt cataatcggg attcggcaca cgtcaaagat
     1681 cgagagtgca tggtcagcgt tggggctcat gaacgagaac cgctgatggc tgacgagagt
     1741 tgatacttcg tggatccgga agattgtttt ccggatggtc cgtcaccgac cacccaccgc
     1801 caagcgccga atagagctgg acgattgaga cgagttggcc ttgcagcgta cgagccaacg
     1861 ccaattccga ttcaaaaaga cttcgctggc tcgtcagcac gtcgaggtaa cttgcccgtc
     1921 caccttgata ccgttgctca gcgaaatgaa acgccgattg cagtgctgcc acttgttgct
     1981 cttgagcttc ccgctgctcc cgtgtctttt gcactgagat gagcgcatcc tctacttctc
     2041 tcaaggcatt cacgatcgcc ttttcatatt gaagcgcagc ctgctgcgct ttggtttcgt
     2101 tgaccgtgac ctggtagccc agcgccgtgg cgttcagcaa cggtcccgcc agattcgcgc
     2161 tgatgttctg aatgaacagg ggtcccaact gattgccgtt gagattcgca ttggctccag
     2221 ccaatgaccc gcctaatgca aactggggaa agcggtttgc ttgcgccacg ccgataccgg
     2281 ccgtcgccga ggccagcgtg tgttcagcct ggagcaaatc gggtcgccgt tgtaagagtt
     2341 cggacggtaa acccgcaggc acctccgggg ggatgggctg ttccgtcaat ttcagaccgc
     2401 gagtaattga cgcaggtctc cgccccagca gcacgcttaa gtgattttcc gtctgcacga
     2461 cttgcttctc gagttccgca agctttgccg cagtgcccgc gcgctccgcc tcgaaccgat
     2521 cgaggtccag cttcgggata tagccctgtt taaatttcag ctgagacagc cggacagatt
     2581 cttcccaggt tttcaacgtc cgcttggtaa tgtccacttg caaatcgagc gatctcagag
     2641 aaaagtaggc ttcactgacg cttcctacca agctaagaat caccgcccgc tgattttctt
     2701 cctgtgccaa gagttgtccc tgcgatgact ccaccgcgcg gcgtagccga ccccagagat
     2761 caatctccca tttcaaactg ccgaagccga tggtgttgga aaaggtcgta ttgtcccccc
     2821 ccgttgtccc cggcaacaca atcgctccgc ctccgcctgg aatggtcgtg gcgttgcctc
     2881 cctgtgtttg atacagaaac gccgtgcccc cataggacaa cgctggtgcc aagtcccatc
     2941 gcgtcatcac gagctgagca cgatattgtt cgaccgaagc gacggctgtc ctcacatcct
     3001 gattctctgt aagggcaatt cgaatcagtt cttgcagttc cttatctttg agaagatccc
     3061 accaggccaa attcgcaatg gattcagccg ttgaggtcgg gacgcgccag gaatcggcct
     3121 ttggagtttc tggacgcaca taatcaggcc ccatcttgca ggcgctcacc atgaacacca
     3181 gcatcccaac gcctgcgaga gaggcggatt gccaggaata tgttcccatg tcatgactcc
     3241 ttaatagctt tcgccttcac gagcgtgagt cggagacaac gcgggtcggg gcgtctgccg
     3301 agacggttct ccatcgccgg ctttctgaga aggagggagc gggggtacct cgctgtgggg
     3361 cgtccctcga tgcacgagac tctcgacgac gtagaatgtg accggaacga ggaagatcgc
     3421 aatgacgctc gcggccagca tccccccgaa aacagtgagt cccagcagga ctcgcccgtg
     3481 cgctccggct cccgtggcaa gcaccagcgg aagcacgccg agaataaatg cgaaggccgt
     3541 catcaaaatc ggacggagtc gcaatcgcgc ccccgccaag gcggcctcag cgatcgactt
     3601 acccttttca aattccactt tggcaaactc tacgatgagg atcgcattct tcgcagccaa
     3661 tccgatcaac atgaccagtc cgatctgggt aaagacgtcg ttctcgctgg cctgtggcgc
     3721 caccagcttc agcagccaca aggcggcgaa cgcgccgaac acggcgatcg gtgtgccgag
     3781 cagtacgctg aatgggagag cccagctctc gtactgcgcc gcgaggatca gaaacacgaa
     3841 gagaagcgag aacccgaaga tcaccgtcgc ggggacgccc tccgcggcag ctttctcttg
     3901 gaaggacatc cccatgtagt cgaggcccat cccgcctggc atcgtgctcg cgaacacttc
     3961 ctctaccgcc ttcatcgcct gtcccgaact gtatcccggc gccgcccctg caatcagttg
     4021 cgccgagcga tactcgttgt atcgcatggt gaactccgga ccatggaaga tgtccatcgt
     4081 caccaaggct gagagtggga ccatatcgcc gttcgcgttc cggacgtaaa actgcccgac
     4141 attttctgcc tgcgtgcgaa actcaccttc cgcctgcacg tagacctgcc agacccgccc
     4201 gaatcggttg aagaagttta ccatcacgcc gcccatgaaa gactgcagcg tctggtagac
     4261 atcggaaagg ttcacgcctt gcttcaagac cttgtcacga tccactctgg caaatacttg
     4321 ggggacggcg ggaataaatg tcgtgtccac tctcgcaagc tcagggcgct tcctggccgc
     4381 ctccataaat ttctgcgtat ggtctgcgag gaattcgatg tcccggccca ttcggtcttc
     4441 caacacaaag gacacaccgc cggacgttcc aactcctggg atcgccggcg gtggaaagag
     4501 gaacgctttg gcctctggta attcagcaag acgcgagttg acattcttga aaatggcagg
     4561 aagctgttct tcggacttct tccgctcttc ccaaggagcg agtgtgacga agaaaaacgc
     4621 gttgtaagtg gtgctcactg tgctcaagag actgaagccg acgattgtgg agacatactc
     4681 aatgccgggt gtcgacttta aaatggcctc gatcttcctg caaacctcat ccgttcgctg
     4741 cagagacgac gcttccggaa gttgaacatt caagtacaca tagccctgat cttccatggg
     4801 aaggaaaccg cttggcagct gtgaaccaag ccagacggct acgatcgtga ccaacagcaa
     4861 aaacaccatc gagcgcccgg cttttcgaat gaggtggccg cagacgctca catatccatc
     4921 cgtggcgcgg ccgaaccatt tgttgaacca gccgaagaag agaccgagcg gcccccgcgc
     4981 cggctttctt ggccgcagca aaagtgctga gagcgctgga ctcagcgtca gggcgttgaa
     5041 cgccgaaatc accaccgaca gcgcgatggt taccgcaaat tgttgataaa gacgtcccgt
     5101 gattcccggt atgaatgccg tcggaacaaa cacggccgcc aaaataagtg cgatggcgat
     5161 caccggccct gagacttctt ccatcgcctt gaggctggct tccttcggag acatgccgtg
     5221 ttcgatgtga tgctcaaccg cttcgaccac cacaatggcg tcgtcgacga ccaggccgat
     5281 agccaggacc aggccgaaca gcgagagcgt gttgatggaa aaccccatca acggaaagag
     5341 ggtaaaggtc ccgatcagcg agacagggac ggccagcaat ggaatcagcg tcgcgcgcca
     5401 cccttgcagg aaaatgtaga cgacgataat gacaagaatc agggcttcga acagcgtatg
     5461 aacgatctcg ttgatgcctt cccgcaccgc ctgggtagta tcgagggccg tgacatagtc
     5521 cagatcgggt gggaactgtg ctttcatctc ctccatcagc ttggtcgcgg catccatcgt
     5581 ttgaatggca ttggagcctg gaagctgata aatggcaatg agcgcggcgg gtttgccgtt
     5641 catccggccg acgatgttgt acatctgcgc gccgagctcg attcgggcca cgtccttaag
     5701 ccggaccatg gatccatcct gattcgcacg gaccacgata ttcccgaatt cttcttcgga
     5761 ttcgaggcgg ccctgtgagc gcacactgta ggtaaactct tgtccgggcg gcaccggttc
     5821 ggcccccacc tgccctgcgg gattcaccgt gttttgagcc ttgatggcat tcgtgatctc
     5881 cgtcaccgta atgcccagct tggcgagatg gtcaggccgc acccagaatc gcatggcata
     5941 ctggccggct ccgaaaatct ggacctgtcc gacgccgggc acacgcgtca tgggatcgtt
     6001 aatgttcacg tagccatagt tcgccaagaa ggtagcgtcg taggtgccgt ttggtgaata
     6061 gagagagaac aaggcaagcg gactcgtcgt ggccttctta atggtgacgc cgaaattctt
     6121 gacgtcggca ggcaattgag attcagcctg ggtatagcgc atctgcgtca ggatctgatc
     6181 gtcgttgggc cttgtgttga tatcgaagtt gactcgcaat gacatctggc cattgttcgc
     6241 gttcaccgaa tacatgtaga gcatgttgtc cacgccgctc atcaattgct cgaccggagt
     6301 ggcaaccgat ttttccagcg tgacggcatc ggctccgacg taggtggctt gcagcaaaat
     6361 ctccggcggc gcgatgttcg gaaacatcgc gatcggaagc tgcaccatcg caatgacgcc
     6421 gagaatcacc atgatgatgg agatcaccat ggcaacaatc ggccgattaa tgaagaattt
     6481 cgccatagga ttgaagactg agttgagaaa tgaggatcag accagcgtta catcatccaa
     6541 gtttttcttc ctgctggtct tggaacggtt tgggatcgac ggtcatcccc gctttgactt
     6601 tttgcaaccc ttcgaccacg actcgctccc cggccttgag tccgtgatca atgatccaca
     6661 aacttccgga ccgctggcca accttgactg gacgaatatc caccttgttg tccggactga
     6721 caaccgccac ctggtaactg ccctgcatct ccgtgacggc acgctgcggg acgaggatcg
     6781 caccctcctt ggtcttggtc accgccctta ctcgggcgaa ttgtccgggc cttaacagat
     6841 tgttgggatt agggaacaac gccgccaccc ggatggtccc ggtcttcgta tcaacctggc
     6901 ggtccaccag atagaattgt cctttatgcg gatagatctt ctctccgccg aggaccaatt
     6961 cgaggagcgc cccgctgtat tccttcccct ctttgcgatc tttatagcct tgctgcactt
     7021 tatctgctgc ctcgaaatat tcctgttcgc tgaccgggaa ataaaccctg atcggatcca
     7081 gcgtggacat cgaggtcaac tcggtgctgg catcgatgag gtcaccgatt tgagcttttg
     7141 aaacgccgac gactccatcg atcggagcgg tgattctcgt ccatgacagg ttcagcttcg
     7201 cctgctcgac ggccgctttc gctgctgcaa ccataccctt ggttgcggcg tgcgcctgga
     7261 tggaatcatc gagctccttt tggctgatgg cgccttcctt cgcaaggggc gtgtcgcgct
     7321 cgacgtcctg tttcgttttc gtgaattgcg cttgggcctt cgccagatca cccatcgcct
     7381 ggtcgagagc tgtgcggaat ttactcggat cgagttcgaa gagtagatcg cccttcttca
     7441 cgaaagaccc ttctgtataa ggccgtttca tgagataccc gccgacctgc gcacgaataa
     7501 gcgcattgtt tgagccgtcc gtggtcccaa cccactccat ttgaatcggc acgtctttct
     7561 ggatgacggt tgcgacctcg acttgcacgg agggaggagg aggggcagaa gccgtttcac
     7621 ccttgcagtg aacgaacgcc gctggagaca ggcttaccag gaccaatgcc gctgatcgag
     7681 ccaggagacg ccctctgtgt gcatggtcca tacgcgctcc ggcaaatgag gaagggggtg
     7741 tttcttatac gctcgccctc ccgaagtcgt caagacgggc gtgctcgacc attcatatca
     7801 cgttgtccgc ttgagactca gcatgtaact cgtgaggtct tggagttccc cctccgtcaa
     7861 tggaaacttc ggcataatcg agccaggcga taccgattgc ggatcacgga agtgcttgat
     7921 gtgccattcc ctgtctggcc ttgcgtcccc
//
LOCUS       DASTQY010000454         4235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1518555, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000454 DASTQY010000000
VERSION     DASTQY010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518555"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VFR82_14590"
     CDS             complement(<1..271)
                     /locus_tag="VFR82_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502876.1"
                     /translation="MNLTFTTLGLVLIAAFLASSAAGSTQDQFLPDCATETYAPENQP
                     TKIRFIVWCGVRSDDVSFSLQRPKDERIVDYGKMLQAWGPGAVAPF"
     gene            complement(300..1325)
                     /locus_tag="VFR82_14595"
     CDS             complement(300..1325)
                     /locus_tag="VFR82_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502877.1"
                     /translation="MKAGARTIIGALAILLSAASTVNADTIGSNLGAPSNASVCKFQV
                     FEPKPQVCTVGQASLSSSHTAGGGLRVPFDGIVVRWSVVSGPALPGTGKVTMALRATQ
                     GPGYMEKGPEVELPLGEPGTRYTFAERLAVTAGQPLGLRIAIENRSVEEAGAPIAFAE
                     PGIGRADLWWGDPESAEWDEEEGVELLLDAEIEPDVDRDGYGDLSQDCFPNHPGDQHL
                     CGRDLVPPTTRSRYAKRQAFLSSGHVVVRVSSNEAGVTRASGDLQIKNKGGSGGRGYG
                     MRTVRKPIAAGGQVALRVRVRPQVLRVARLAAAEGRKVVVTGRVGVVDASGNEGQAPF
                     RIRLKAN"
     gene            complement(1936..>4235)
                     /locus_tag="VFR82_14600"
     CDS             complement(1936..>4235)
                     /locus_tag="VFR82_14600"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEU4502878.1"
                     /translation="FSADGDYIAGFGGSGSGAQQLDLKNKTGIAASLGRVWVADGGNH
                     RLAEWAGGIYEPSEEPVLVEDDPQLEVNVSEGLVDSVEGEESGAIDYDHSGDLLTAVD
                     APDGEAQFSYDGAGRMTKVSLPNGTYGEIAYEATYGRVKSVTTAIEGKNPKTTHFSWS
                     DEPHRTTVTPPDAPATTYDIAADGSIFKWWNAKQPPVFDDVAGTLFQNRETSTPISTG
                     VHNLVIQAHDEEGVASIQVIANNNQLVDERTCSYDPEKPTECVTETNEWVTETGNWPP
                     GIVYLEVIATDRLGEASSQRFWVNIPYTPPPDPEAEEPPRFGDILRFREEFGLDLDLK
                     GDELAINDRVFELMGDWNNPLTPAGEVARATNEKWGVPLRALDAAELEYRQSYVDQAS
                     THLKQWVNSSGVENTYAGYYVDDRAGGIVRLGFTNNQENLVNLFKSDMPFLPSDRIQA
                     FQASPGHSLGTLEALATDLRSRRINEPAITEVVVNSRTNAVDVGASNVPLVQSAVNGW
                     YGQSAPISVRYLANADEPKDVRERLSGPMRAGDRVVDDHWQCTLGFGARESTGEKSNG
                     EAVVLYFGLTAAHCFETVGEPVFRTGLNSEGQYKEVRIGNMTRTGFGVNFNGYGTDGQ
                     AVKLKEPGLVPKWIQVENFYTQKIHGAAPLKENMIVCTTGITSGTASCGRVRLFEEVE
                     YNGRVMLQFNVQAAGPEGNSGGPVWDLMTGAAVGLTSSGPEGPAEWRETNVTPLLPVP
                     GAPNNGVPGILAAPGMGALSIVAAK"
BASE COUNT          800 a         1316 c         1193 g          926 t
ORIGIN      
        1 gaaatggcgc aacggctcca ggcccccatg cctgaagcat ctttccgtag tcgacgatgc
       61 gctcatcctt gggtcgctgc aaggagaagc tcacatcatc gctccgcaca ccgcaccaga
      121 cgatgaaccg aatcttggtc ggctgatttt caggcgcata agtctcggtg gcgcaatcgg
      181 gcaggaactg atcctgcgtt gaaccggccg ccgaactggc caagaatgcc gctatgagca
      241 cgagaccaag cgtggtgaag gtgaggttca aggccctgtt tatcggccgg tccacaacgt
      301 caatttgctt tcaatcgaat ccgaaatggg gcttgtcctt cattcccgga cgcatcgacg
      361 acaccaacgc ggccggtgac cacgactttc cttccttcag ccgcagccaa ccttgccacc
      421 ctgagcacct gcggccggac tcgaacacga agcgcaacct gcccgccggc agcaatcggc
      481 ttgcgcacgg tgcgcattcc gtagcctcga ccgcccgagc ctcccttgtt cttgatctgt
      541 agatctcccg aggctcgagt gacccccgcc tcattggaac tcacccggac cacgacgtgg
      601 ccggaggaaa ggaacgcctg ccgtttggca tatcgcgacc tagtggtggg cggcacaagg
      661 tcacgtccgc acagatgctg atctccagga tgattcggaa agcaatcctg agagaggtca
      721 ccgtatccat cgcggtcgac atccggctcg atctcggcgt ccagcaggag ctccacgcct
      781 tcttcttcgt cccactccgc cgactccgga tcgccccacc acagatcggc cctcccaatc
      841 ccaggttcgg caaatgcaat cggggcgcca gcttcctcaa cgctccgatt ctcgatggcg
      901 atccttaggc cgagaggctg ccccgcggtg accgccaacc tttcggcaaa cgtgtagcgg
      961 gtacccggct cacccaaggg aagctcgacc tctgggcctt tttccatata tccgggtccc
     1021 tgagtagccc ggagcgccat cgtgaccttc cccgtaccag gcaacgccgg cccactgacg
     1081 acggaccaac ggacgacgat gccgtcgaag ggcactctga gacctccacc agcagtgtga
     1141 ctagacgaga gactggcctg gccaacggtg cacacttggg gtttcggttc aaacacctgg
     1201 aatttgcaca ccgaggcgtt tgaaggcgcg cccaaattgc tacctatggt gtcggcgttg
     1261 accgtactcg ctgccgacag caaaatcgca agcgcgccaa tgatggttct tgcccctgct
     1321 ttcatcattc tccttgaccc cgtttgagct tcacatgatg cgagatctcc ttcaggggaa
     1381 ccatcgcttt tcgccacacc ttggaagctc cgaactcttt caagccaccc attagggtcc
     1441 aaggctattg cggacgaacc tggaacagag cacttcgatg cgctgccttt cgctcaatct
     1501 gtacgggaac ggtaaagcgg tagcggcccg caggctgact gtcgggaacg gtaaaccgga
     1561 agcaagtact gagctcacct ggatttagta tcccgagctt tttgggccat ggtcccttgg
     1621 ggctggcggg atccagagac catttcccat gcaccaatct ttcgatcctt aaagcaacgc
     1681 cgtatcctgc cttcctcttg cctcgattga aaagtcgcgc agtagcgact cccccaggat
     1741 ctaccacgtc gttcttcatt gccagggaca cgtcctgagt tcttgatccc gtggctcgct
     1801 cttccatcct gcgcaggtcc gtcgtttggc aaaaagaact ttcagatcgt gagtctgcgg
     1861 gcacagggga agcgagcaaa actgctaata cggccattcc agccacccaa gcagaaataa
     1921 atgtgccact agccctcatt tcgccgccac aatgctgagg gctcccatgc ctggcgccgc
     1981 aagaatgcct gggaccccgt tattcggcgc tcctggaacg ggcagcagcg gagtcacatt
     2041 ggtttctctc cattccgctg gcccttccgg ccctgaggac gtgaggccaa ccgccgcacc
     2101 cgtcatcaga tcccaaactg gaccgccact atttccttct ggtccggcag cttggacgtt
     2161 gaactgcaac atcacacgac cgttgtattc gacttcttca aacaatctca cacggccgca
     2221 tgaggcagtc cctgaggtaa tgccggtcgt acacacaatc atgttttctt tcagcggtgc
     2281 ggccccatga atcttctggg tgtagaagtt ctcaacctga atccacttcg gaaccaagcc
     2341 aggttccttc aacttgactg cctgaccatc cgtaccatat ccgttgaagt tgaccccaaa
     2401 gccagtcctc gtcatattgc cgattcgcac ctctttgtat tgtccctcac tattcagacc
     2461 agtgcgaaac acaggctcac caacggtttc gaagcagtga gcggcagtta gaccgaagta
     2521 cagaactacc gcttcgccgt tgcttttttc accggtggat tctctcgctc cgaagccgag
     2581 ggtgcactgc cagtggtcat ctacaacacg atcaccagcg cgcatcgggc cactcaggcg
     2641 ttctctgacg tccttgggct catcagcatt cgctagatac ctcaccgaga tgggtgctga
     2701 ctggccatac cagccattga cggctgactg aacgagcgga acattgctcg cccctacgtc
     2761 aacggcattc gttctgctgt tgaccacgac ctcggtgatt gcaggttcat ttatgcgccg
     2821 acttcttagg tcggtcgcga gtgcttctag agttcccaag ctgtggcctg gggaagcctg
     2881 aaaggcttgg atccggtctg acggcaagaa gggcatatcg ctcttgaaga gattaaccaa
     2941 gttttcttga ttattagtaa agccgagccg gacaatcccg ccagcccgat catctacgta
     3001 gtagcccgca tacgtattct cgacaccaga gctatttacc cactgcttca agtgggtgga
     3061 cgcttgatcg acataggact gccggtattc cagctcggct gcatccagcg cccgcagcgg
     3121 aactccccat ttttcattcg ttgcccgcgc aacttccccc gctggcgtca gggggttatt
     3181 ccaatccccc atcagttcga agacacggtc gttgatcgcc agctcgtcgc ctttgaggtc
     3241 taggtcgagg ccgaattctt cgcggaacct gaggatgtcg ccgaaacgtg ggggttcttc
     3301 ggcttcggga tcaggtgggg gcgtgtaggg gatgttgacc cagaagcgtt ggctgctggc
     3361 ttcgccgagg cggtcggtgg cgattacttc gaggtagacg atgcccgggg gccagttgcc
     3421 ggtttcggtg acccactcgt tggtctccgt gacgcattcg gtgggttttt cggggtcgta
     3481 ggagcaggtg cgttcgtcga cgagctggtt gttgttggcg atgacctgga tcgaggcgac
     3541 gccctcctcg tcgtgggcct ggatgacgag gttgtggacg ccggtcgaga tcggcgtgct
     3601 cgtttcccgg ttctggaaca gggtgccggc gacgtcgtcg aagaccggcg gctgttttgc
     3661 gttccaccat ttgaagatcg agccgtcggc ggcgatgtca taggtggtgg ccggcgcgtc
     3721 gggcggagtg accgtggtcc ggtgcggttc gtcggaccag ctgaaatggg tggttttcgg
     3781 gtttttgcct tcgatcgcgg tggtcacgga tttgacccgg ccataggtcg cttcgtaggc
     3841 gatttccccg taggtgccgt tgggcaggct gactttggtc atccgcccgg cgccgtcgta
     3901 ggagaactgc gcttcgccgt cgggcgcgtc gaccgcggtc agcaggtcgc cgctgtggtc
     3961 gtagtcgatc gcgccgctct cttcgccttc gacgctgtcg accagccctt cggaaacgtt
     4021 gacctcgagc tgcggatcgt cttcgacaag gaccggttct tcggagggct cgtagatgcc
     4081 gccggcccat tcagccagcc ggtggttccc cccgtcggcg acccagaccc ggcccagcga
     4141 cgccgcgatc ccggttttgt ttttgaggtc cagctgctgg gccccggaac ccgagccgcc
     4201 gaagccggcg atgtagtcgc cgtcggcgct gaaga
//
LOCUS       DASTQY010000455         8337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_257231, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000455 DASTQY010000000
VERSION     DASTQY010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8337
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257231"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..682)
                     /locus_tag="VFR82_14605"
     CDS             complement(104..682)
                     /locus_tag="VFR82_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU4502879.1"
                     /translation="MRPEQGTLISRAQAMAALLELLTDQPVIICNGFPSREAQKLADR
                     PTHFYMIGSMGSAPAIALGVALAKPHKQVITFDGDGNVLMGMGTLATVAALKPKNFIH
                     VVFDNEVYGTTGNQPTISNVVPLDKVAKAAGYTNVERVLDLDDLVYEFKDMLKKDGPS
                     MLLIKVNEFAEDAGRVVLDPPDITKRFMKAIE"
     gene            complement(679..1176)
                     /locus_tag="VFR82_14610"
     CDS             complement(679..1176)
                     /locus_tag="VFR82_14610"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU4502880.1"
                     /translation="MIESDAFVQAMQDMGVNFFTGVPDSMLGGIIAELMDRRLYTPAV
                     REDEAVAVAAGAYMGGKIPAVLMQNSGLGTSLNTLISLNLIYHQPCILIVSWRGQGGK
                     DAPEHLVMGEVMPQLLDTMKIPHRTLTERTALEDFKWVAETFMEQRIPVALFITKGVV
                     KGLHP"
     gene            1299..1802
                     /locus_tag="VFR82_14615"
     CDS             1299..1802
                     /locus_tag="VFR82_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502881.1"
                     /translation="MRLFFFLVGMIAFSPFPAGAVSMTNDPKGFHNIVWGTALNTRPD
                     LELTRQGRNIIEYQFKDTPPSFASIPVESLRLSTIDDQFARVTIRYRGAETHKKILSY
                     LESEFGRTEHLPGQMLRGLNQQYNWRGSESEINLTYQSSTERGYIFLDSRTLAPRFND
                     LLADTAE"
     gene            1806..2354
                     /locus_tag="VFR82_14620"
     CDS             1806..2354
                     /locus_tag="VFR82_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502882.1"
                     /translation="MSIRDFLLLQVFLGMLLSGNLLVVTATLAVEMKEDPKGFEGIPW
                     GATLSESDTFGKVEDAGRLKTYELKGGPPTFGPAKVESMRFTTVDDQFARVTVRYHGR
                     EAHDQLLGFLQSRYGPLDRTPGQFAGGAVKIFDWQGLETVVSLRYETRSDRGIIFFEN
                     PALATRIEGAMAPDQDLGGATY"
     gene            2420..4099
                     /locus_tag="VFR82_14625"
     CDS             2420..4099
                     /locus_tag="VFR82_14625"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEU4502883.1"
                     /translation="MHNATKLRAALKKNGALKVVGAHDALSARLIERAGFDGVWASGF
                     AISASLKCIPDASFITSSEQLEVERNIAEAVNIPVIADCDTGYGNALNVMRTVNDRER
                     AGVAAICIEDNVYPKRCSFYAGVRRELIPIDEHCGKIKAAKAAQTVPDFMVIARTEAL
                     IAGWGQEEALKRAEAYAEVGADAVLIHSKSKTFDELKTLYKAWSRRVPLVVVPTIFDQ
                     TTAQEMEQAGAKIIIYANQPVRAAIRAMRDTLDLIRSDTRPGAANDQIVTLPEVYDIV
                     GVPQMEQDEKAFLPIGGEKITAIIPAAGFEKQLLPLIEDKPKCLLDIKGKTILERAVA
                     ALNEANIKEIAVIRGYKKDAIALPNIRYYDNDRYEETGELFSIFCAESEMKGRTIVLY
                     GDIIFDTTILEKLLKSPADFALVVDLAWRDHVERGGAPSHLNPDLVTLADPPGSSYLS
                     RFVMGEDEHRIVKIGQHLPLDQVHGEFIGMAMFSEKGTQAVRECYRTSQERYKSAGFH
                     EAGSVSKASFTDLVQELIDEGHHIDAVPVFKGWMEVDSFEEYQKAWAKIRQ"
     gene            4252..4668
                     /locus_tag="VFR82_14630"
     CDS             4252..4668
                     /locus_tag="VFR82_14630"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4502884.1"
                     /translation="MKCSLLEQNLAARARAPLDRRYAERALIHHEVTYTSTEGARFTT
                     SIGSLKDLSKTGCKILGTTLPAIGGHITVTLYLNDGQAPLSLTDATVSWIKGHVFAVR
                     FPKLTDDERKRIQGIIWKHVRLPQANHLRTAFRIVP"
     gene            4721..5116
                     /locus_tag="VFR82_14635"
     CDS             4721..5116
                     /locus_tag="VFR82_14635"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4502885.1"
                     /translation="MKHTSIDRSRRTAGKSSDDNRRGIERVPTQFGLMYSGMCDGRML
                     IGNGVVRNISQVGIGIHGNQVVKLGMDLSLFIDLPGIEEPICIAESRVSWVSGRRFGV
                     EMIAPRLEVQNELRFYVWNHLFRQKANKS"
     gene            5131..5511
                     /locus_tag="VFR82_14640"
     CDS             5131..5511
                     /locus_tag="VFR82_14640"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4502886.1"
                     /translation="MKQERGRVSKTLHRTHPICDRRTEHRVPTDVRLMYSAQDEKNDL
                     IMGDGSVVDLSNTGLRIRGNVAVKSGLEVTLFLYLPDGRDPLFVLSTRVAWSRGHVFG
                     VEILKMNVRERNRLRYFLGTNLSR"
     gene            5610..6224
                     /locus_tag="VFR82_14645"
     CDS             5610..6224
                     /locus_tag="VFR82_14645"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU4502887.1"
                     /translation="MATRSTSQQRQADLIAAAASLFAAKGFNGTTTKEIAKAAGVSEA
                     LVFKHFPTKRALYGAILAEKVTLDELLGAVEESARKRDDRRVFTLIAGFRIKPAVDST
                     LLRLLLFSALEGHELSDMFFGKHHRVLYDHLATYIKTRIDEGAFRDVDPLLAARAFIG
                     MVVHHRLLHEIFAVPMHQTHDATVRTYVDLFLTGITKARRSRPL"
     gene            6221..7396
                     /locus_tag="VFR82_14650"
     CDS             6221..7396
                     /locus_tag="VFR82_14650"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU4502888.1"
                     /translation="MNPVKQHPLVTLGIIIFFAVTALVVFRLSSGAKIDQKKSRVITV
                     GTITPLQQDLEIRLAYTADITPNQVVNVFSRVDGYISKLHVDKGDFVKANQLLLEIDH
                     QDYLHNVNQAKANLAAARAKVTQQEASVRNAKLTLDRMQALIKDQFVSQQDVDTAEVN
                     SDAAIAALESLRAQVKQMEVALAQAETNLTYSYIRAPFSGYVAERNLDLGAYVTGAAT
                     SNSTTSRGIVSLHDIETVRTLIEVVEKDVPLVRIGQKAEIRAEAYPDRVFEGTVTRVV
                     QALNRATRTMTVEVDLPNKDRLLKGGMFARVEVLVGKHQNALQIPIDAVSRLEDAQYV
                     YIVRDGKAERVSIEIGVRDENRVEITKGLVGSEQVIVSGKDLVHDGTPVQTEPLTRS"
     gene            7473..>8337
                     /locus_tag="VFR82_14655"
     CDS             7473..>8337
                     /locus_tag="VFR82_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU4502889.1"
                     /translation="MWLTLLALRNRIGILMLSLAMVVLGATSLNRLPVDLFPNIQVPV
                     AFVGVIYKGAPPLDIEQSVVYPIEKAVSSASNVEHVESFAKQGIGAVQIWFNWGADIN
                     VGQMEVMQRITQILNSLPPGILQPFIVKFDVSNIPVSFVTVSSDALDERALYDLAFNT
                     IAPQIEQIANVAAATVEGGKIRQININLDPALLNARGLSILDVVKSVKAANLILPSGD
                     IKAGNLDYNVFTNNQFRTVEPIQDVIVKVNQQGNPVRVRDLGTVTDSSDIQTNIVRTD
                     GKRAVYLRVNKQ"
BASE COUNT         1910 a         2369 c         2289 g         1769 t
ORIGIN      
        1 agaaccgagc cccgacgatc gaggtgtggc gaaaccgggc gacgagcact caatgggggc
       61 tgtgaaaagt ccagtcatag gcaagtccat ggggttagcg gtcttattct atcgccttca
      121 taaatcgttt tgtgatatcg ggagggtcca gcacaactcg tcccgcatct tctgcgaact
      181 cattgacctt gatcaatagc atactcggtc cgtccttctt gagcatatct ttgaactcat
      241 agaccagatc atcgagatcc agtacacgct cgacattcgt gtagcctgcc gccttggcga
      301 ccttatccaa aggcacgaca tttgagatcg tcggttgatt acctgtcgta ccgtacactt
      361 cattgtcaaa gaccacgtgg atgaaatttt tcggcttcag ggcggcgact gtcgccaacg
      421 tgcccatacc catcaagaca ttcccatccc cgtcgaacgt aatgacctgc ttgtgtggct
      481 tggccagggc gacgcccaag gcgatagccg gagcgcttcc catggaaccg atcatgtaaa
      541 aatgggtcgg ccggtcggca agtttctgag cctcgcggga agggaaaccg ttgcagatga
      601 tcaccggttg gtcggtcaat agttcgagca gcgccgccat cgcctgcgca cggctgatca
      661 atgtcccctg ctccggcctc atgggtgcaa ccctttgaca acccccttgg taatgaacaa
      721 agcgaccggg atccgctgtt ccatgaaggt ctccgctacc catttgaagt cttcgagcgc
      781 ggtgcgttcg gtcagcgtcc ggtgcggaat tttcatcgta tccaacagtt ggggcatgac
      841 ttcgcccatg acgagatgct cgggggcgtc cttccctccc tgcccgcgcc aagacacgat
      901 caggatacaa ggctgatgat agatgaggtt cagggaaatc agtgtattga gtgaggtccc
      961 caatcccgaa ttttgcatca gcacggcagg tatcttcccg cccatgtaag ctccagcggc
     1021 cacggccacg gcttcatcct cacggacggc cggggtatac agtcgccggt ccatcagctc
     1081 ggcgataatg cctccgagca tggaatcggg aacccccgtg aagaaattca cacccatatc
     1141 ctgcatggcc tgtacgaagg catcgctctc gatcattctg acctttcttc gagaatcata
     1201 tcgtgccgaa aaaaggcgca ttataagcca tgccgtgagg atttcacaag gaaacgaagc
     1261 ccgttgcgtc actccataca gcatgatagc gttacaacat gcggctattc ttctttcttg
     1321 tgggcatgat tgcattctcg ccttttccgg ccggcgctgt gtcaatgacc aacgatccga
     1381 agggatttca caatattgtc tgggggactg cattgaacac acggccggat ctggaattga
     1441 cacggcaagg ccgtaacatc atcgagtatc agttcaagga tacgccgccg tcgtttgcga
     1501 gcattccggt cgagagcctt cgactgtcaa ccatcgacga ccaatttgcc cgcgttacca
     1561 tccgctaccg cggcgcggaa acgcacaaaa agatcctctc gtatctggaa tcggaattcg
     1621 gtcggacgga acacctgcca ggacagatgc tgcgcggcct caatcagcag tacaattggc
     1681 gcggcagcga gtcggaaatc aatctgacct accagtcgag caccgaacgc ggctatattt
     1741 tcctggacag ccggacgctg gcgccacgat tcaacgatct gctggccgac acggcggaat
     1801 agcccatgtc tatccgggac tttctcctgc ttcaggtctt cctcgggatg ttgctttcag
     1861 gcaacctcct ggttgtgacc gcaacgctag cggtcgagat gaaggaagac cccaaaggct
     1921 ttgaaggcat cccctgggga gcaaccctgt cggagtctga cacgttcggt aaagtagaag
     1981 atgcgggccg gttgaagact tatgagctca aaggaggacc gccgacattt gggccagcca
     2041 aggtagagtc catgagattt accaccgttg atgaccaatt cgcccgcgtc acggttcgct
     2101 atcacggtag agaagcccat gatcaactcc tgggatttct ccagtcacgg tatgggccgc
     2161 tcgatcgcac gccgggacag tttgcgggtg gcgccgtaaa gatttttgac tggcaggggc
     2221 tggaaaccgt ggtgagtctc cgctacgaaa cccgctctga tcgtggcatc atctttttcg
     2281 aaaatccggc gcttgctacc agaattgaag gcgccatggc ccccgatcag gatttgggcg
     2341 gtgccacgta ctaattcgat cgctgcgcaa aacttttcat tgacaacaac attcggcttt
     2401 cgcatcatgc cccctcatca tgcataacgc caccaagcta cgagcggccc tgaaaaagaa
     2461 tggagctctc aaggtcgtcg gggctcatga cgcgctcagc gccaggttaa tcgaacgggc
     2521 cggcttcgat ggcgtttggg ccagcgggtt tgccatttcg gcctcgctca agtgcattcc
     2581 cgacgccagt ttcatcacgt cgagtgagca actcgaggtg gaacgtaaca ttgcggaagc
     2641 ggtgaacatc cccgtcattg cggactgcga caccggatac ggcaatgcct tgaacgtcat
     2701 gcgcaccgtc aatgaccgtg agcgggcggg ggttgctgcg atttgcattg aggacaacgt
     2761 ctaccccaag cggtgtagtt tttacgccgg cgtccgccgg gagttgatcc ccattgatga
     2821 acattgcggg aagattaagg ctgccaaagc ggcgcaaacg gtgcccgatt tcatggtcat
     2881 cgctcggacc gaagccttga ttgccggttg gggccaggag gaagcgttga agcgcgccga
     2941 ggcctatgcc gaggtcggtg ccgatgccgt attgattcac tcaaaatcca aaacgttcga
     3001 cgagcttaag actttataca aagcctggtc caggcgcgtg ccgttggttg tggtcccaac
     3061 catttttgat caaacaaccg ctcaagaaat ggagcaggcg ggtgccaaga tcatcatcta
     3121 tgcaaatcaa cctgttcgag cggctattcg ggccatgcga gacaccctcg atctcatccg
     3181 gagcgacacg cggcccggtg cggccaatga tcagatcgtg accttgcccg aagtctacga
     3241 cattgtgggc gtacctcaaa tggaacagga cgaaaaggcc tttcttccga tcggcgggga
     3301 aaaaattacg gcgatcattc ccgcggccgg attcgaaaaa cagctgctcc cgttgatcga
     3361 agacaaaccc aagtgcttac tggatatcaa aggcaaaacg attctcgagc gtgccgtggc
     3421 tgcgctcaat gaagcgaata tcaaggagat cgccgttatc cgaggataca agaaggatgc
     3481 catcgcactg ccaaatatcc ggtactacga caacgatcgt tatgaagaga cgggagagct
     3541 cttttccatc ttctgtgccg aatccgaaat gaagggccga acaatcgtgt tgtacggcga
     3601 cattattttc gataccacca tcctggaaaa actgctgaag agccccgccg atttcgccct
     3661 cgttgttgat cttgcctggc gggatcatgt ggaacgtgga ggggcaccct cccatctcaa
     3721 ccctgatctt gtgaccttgg ccgatccacc cgggagtagc tacctgtcgc gattcgtcat
     3781 gggagaggac gaacaccgga tcgtcaaaat cggtcagcac cttcctttgg accaagttca
     3841 tggagagttc attggcatgg cgatgttttc cgagaaggga acgcaggccg tgcgggagtg
     3901 ctaccggacc tcacaagagc ggtacaagtc tgccggcttt catgaagccg gcagtgtgag
     3961 caaagcttcc ttcaccgatc tggtgcagga gttgatcgac gagggacacc atatcgatgc
     4021 agtgcctgtg ttcaaaggct ggatggaggt agattcattc gaggagtatc agaaggcctg
     4081 ggccaaaatc cgccaatgac tgccaagtaa aactacgtag tcttcgttca tccatacacg
     4141 gctctactgg tccttgcccc tcttctgtag gagagcagtt cccccctatt taggaatggc
     4201 cgaacctcgc cactcgggta ctctcgaggt gtacgcaacc aggaggcacc gatgaaatgc
     4261 agcttgcttg aacagaactt ggcggcgcgc gctcgtgcgc cgcttgatcg gcggtatgcc
     4321 gaacgagcgc tgatccacca cgaggtcacg tataccagca ctgaaggagc cagattcacc
     4381 acatcgatag gctctcttaa agatctctca aaaaccggct gtaaaatcct cggcacgacg
     4441 cttccggcga tcggaggcca catcacggtc acactgtatt tgaacgacgg gcaagcgccc
     4501 ttatctctga cggacgcgac cgtatcctgg atcaaaggtc atgtcttcgc cgtgcggttc
     4561 cccaagctga cggacgacga acggaagcga atacagggca tcatctggaa acatgtccgg
     4621 ttgccccagg ccaaccacct ccggacggcc tttcgtatcg ttccctaaac gacacggaaa
     4681 acggcacgga gactcgatct gaccattgga agggaaagac atgaaacaca cgagcattga
     4741 ccggtcacgc cgaaccgccg gcaagtcatc tgacgacaac cgtcgcggga tagagcgcgt
     4801 cccgacacag ttcggcctga tgtactccgg gatgtgcgac ggtcgcatgt tgatcggcaa
     4861 cggcgtcgtg cgcaatatct cacaagtggg catcgggatc catggcaacc aggtagtgaa
     4921 acttggaatg gacttatcgc tctttattga cctccccgga atcgaagaac ctatatgcat
     4981 cgctgaaagc cgtgtctcat gggtgtctgg acgacgcttc ggagtcgaga tgattgcgcc
     5041 cagactcgaa gtccagaatg agctgcggtt ttatgtgtgg aaccatctgt ttcggcaaaa
     5101 agccaacaaa tcttgatcag aggtgacgcc atgaagcagg aaagaggtcg agtgtcgaaa
     5161 acactgcatc gcacccatcc catttgtgac agacgtacgg agcatcgggt tcccaccgat
     5221 gtccggctca tgtattcggc acaggacgaa aaaaacgact tgatcatggg tgatggcagc
     5281 gtcgtcgacc tttccaatac ggggctgcgc atccgtggca acgtggccgt aaagtccggc
     5341 cttgaggtga cgttgttcct ctatcttccc gacgggcgcg accccctctt cgtcctgagc
     5401 accagggtcg cctggtcgag ggggcatgtg ttcggagtgg aaatcctcaa gatgaacgta
     5461 cgggaacgga atcggttgcg gtactttctg ggcaccaatc tttctcgata aactccctcc
     5521 ggctcggaga agcagtcttt tcattgtccc ccgccagatg ttcgtgatac cctctaggta
     5581 agtaattact cactcactta gtgacgttta tggccacgcg atcaaccagt cagcagcgtc
     5641 aggccgacct cattgcggcg gccgcctcgc tctttgcagc caagggattc aacggaacga
     5701 cgacgaaaga aatcgccaag gcggccggcg tcagtgaagc gctcgtgttc aagcacttcc
     5761 cgaccaagcg cgcactgtac ggagcgatct tggctgagaa ggtcaccctc gatgagctgt
     5821 tgggagcggt cgaggaatcg gcgcgaaagc gtgatgatcg gcgggtgttc accttaatcg
     5881 caggctttcg catcaaacct gcggtggatt caaccctctt gcggttactg ctttttagcg
     5941 cgctcgaagg ccatgagttg tccgacatgt tcttcggcaa gcaccatcgc gtgttgtacg
     6001 accacctggc gacctatatc aaaaccagaa tcgacgaggg cgcttttcgt gacgtggatc
     6061 ctctgctggc cgcccgggcc ttcatcggca tggtcgtgca ccatcgtctc cttcacgaaa
     6121 tttttgccgt ccccatgcac caaactcatg acgcaacggt gaggacctac gtggaccttt
     6181 tcctcacggg tatcacgaag gcacggcgga gccggccact atgaatcctg tgaagcagca
     6241 cccgctcgtc acgctcggca tcatcatctt ctttgccgtg acggcacttg tcgtattccg
     6301 cctgagcagc ggcgccaaga tcgaccaaaa aaaatcacgt gtcatcacgg tgggcacgat
     6361 cacccccctg cagcaagacc tggagatccg gttggcgtac accgctgaca tcactcccaa
     6421 ccaggtggtc aatgttttct cacgtgtcga tggttatatc tcgaagctgc atgtggacaa
     6481 gggcgacttc gtgaaggcga atcagttgct tctggaaatc gatcatcagg attatctgca
     6541 caacgtcaac caggccaaag ccaacctcgc ggcagcccgg gcgaaggtca cccagcaaga
     6601 ggcatcagtt cgcaatgcca aactcacgct tgaccgcatg caggccctga tcaaggatca
     6661 gttcgtttcg caacaggatg tggacaccgc cgaagtgaac tcggatgcag cgatcgccgc
     6721 cctcgagtcg ctccgcgccc aggtcaagca gatggaggtc gcgctcgcgc aggcagaaac
     6781 caacctaacg tattcctaca tccgtgcccc cttctcagga tatgtagcag agcggaacct
     6841 cgatttaggc gcctatgtga cgggagccgc caccagcaat tccaccacgt cccgcgggat
     6901 agtaagtttg catgatatcg aaacggtccg taccctcatc gaagtcgtgg agaaggatgt
     6961 tccgctggtg cggatcggac agaaggcgga gatccgcgcg gaagcgtatc cggaccgagt
     7021 gttcgaaggg acagtgacgc gcgtcgtcca ggccttgaac cgcgccactc ggaccatgac
     7081 ggtggaggtg gacctcccga acaaagaccg cttgctgaaa ggcggtatgt tcgcgcgcgt
     7141 ggaagtcctg gtcggaaagc accagaacgc attgcagata cccattgatg ctgtcagccg
     7201 attggaagat gcccaatacg tgtacatcgt acgcgatggg aaggccgagc gtgtatcgat
     7261 tgaaatcggc gtccgggacg agaaccgggt cgagattaca aaggggctgg tcggctccga
     7321 acaagtgatc gtgtcaggaa aggacctcgt tcacgacggg acgcccgtac agacggaacc
     7381 cctgacccgc tcgtaagccg tctgggttgg cacggatcag agcattttga ggtagatcga
     7441 tcctgctgag ccttcgctca gcgcacgaca ttatgtggct cacccttctc gccctacgaa
     7501 atcgcatcgg catcctgatg ctgtcgcttg cgatggtcgt gctcggtgcc acctcgctga
     7561 atcgcctccc cgtcgatctg ttcccgaaca ttcaggtccc tgtcgccttt gtcggtgtca
     7621 tctacaaagg cgccccgcca ctcgatatcg aacagagcgt cgtgtatccc atcgagaaag
     7681 ccgtcagctc ggcgtccaat gtggagcatg tcgaatcatt cgccaagcaa ggcatcggcg
     7741 ccgttcaaat ctggttcaac tggggcgcgg atatcaacgt cggacagatg gaggtcatgc
     7801 agcggattac ccagatcttg aacagcctgc cgccgggtat tcttcaaccg tttatcgtca
     7861 agttcgacgt ctcgaatatt ccggtgtcct tcgtcactgt ttccagcgac gcgctggacg
     7921 aacgggcgct gtatgacctt gcgttcaata cgatcgctcc ccagattgaa cagattgcca
     7981 acgtcgccgc cgccaccgtg gaaggcggca agatccgcca gatcaacatt aatctggatc
     8041 cggcactgct caatgcgcgt gggctgtcga tcctggatgt tgtgaagtcg gtcaaagccg
     8101 cgaatttgat cctcccttct ggcgatatca aggccggcaa tctcgactac aacgtcttca
     8161 ccaataacca attccgcacc gttgagccga ttcaagatgt cattgtcaaa gtgaaccaac
     8221 agggaaaccc tgttcgggtc agggacctgg gcactgtgac cgattcttcc gacatccaaa
     8281 cgaacatcgt tcgaacagac ggcaaacggg cggtgtacct tcgcgtcaac aagcaac
//
LOCUS       DASTQY010000456         3035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1317014, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000456 DASTQY010000000
VERSION     DASTQY010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317014"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..830
                     /locus_tag="VFR82_14660"
     CDS             39..830
                     /locus_tag="VFR82_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEU4502890.1"
                     /translation="MSQYRIEKEPYYADVRGEVDLFLTAAKSRMPVMLKGPTGCGKTR
                     FVQYMAYRLGRPLITVACHEDLTASDLVGRYLLKGQETVWVDGPLTLGVKQGAIVYLD
                     EVVEARKDTTVIIHPLSDDRRLLPIEKKGQAIEAAENFLLVISYNPGYQNVLKDLKPS
                     TKQRFVAIEFDYPTADIEASIVEREAGIAHDLAVRLVKLGGKIRHLKNHGLEEGVSTR
                     LLIYAGTLIREGIAIERACDVAIARPITDDADMQRSIMELVKAMF"
     gene            959..1249
                     /locus_tag="VFR82_14665"
     CDS             959..1249
                     /locus_tag="VFR82_14665"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HEU4502891.1"
                     /translation="MKDWQGGILLTVFAQPKASRTAWVGLRGDAVKIRIAASPVDGAA
                     NRELIRFLAEELSMPMSAIDIHSGAGGRHKRVWVRGTTAVDVTAYLIRKCQG"
     gene            1252..1677
                     /locus_tag="VFR82_14670"
     CDS             1252..1677
                     /locus_tag="VFR82_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HEU4502892.1"
                     /translation="MNLFRALVLMVGTLLCACSGPKPVLYPNAHLQSVGNETAEQDIE
                     ACKEAAETAGADEGSGKAGRVATSTGVGAGVGAASGAVGGAISGAAGQGSLIGAASGA
                     VWGLLTGLFTAGSSQPSQAFTNYVNRCLQERGYEVTGWQ"
     gene            1772..2194
                     /locus_tag="VFR82_14675"
     CDS             1772..2194
                     /locus_tag="VFR82_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502893.1"
                     /translation="MNKVLISWLVIALAGCSGPEPMLRANAKLLLKGREASHLEVAAC
                     QQKAEAAGLQPGTAGRAGNMAAGAGLGLIAGAAVGASTGVIGGATGVAIGAAAGAGLG
                     LIIGLAGGAYRPLEPDPPYADAVVRCLIEQGYEVTGWH"
     gene            complement(2196..2609)
                     /locus_tag="VFR82_14680"
     CDS             complement(2196..2609)
                     /locus_tag="VFR82_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HEU4502894.1"
                     /translation="MAEPKQASLDKMWKYVNSFAAKSGTTMHPNRAVTESVVKGLAMH
                     VDELGKPLCPCNFYKDKAAEAKRRRWICACDEMQIYKYCHCLLFVREDGMPITEYLPE
                     GHEGREVYGVITDPTPDKGRALRHKASDASPDTTD"
     gene            2838..>3035
                     /locus_tag="VFR82_14685"
     CDS             2838..>3035
                     /locus_tag="VFR82_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502895.1"
                     /translation="MESANLQLINRLAGELDAVEAAALVPRIVAAAHKPAAILALLDE
                     LEAISAKAVRAAIEALPELDRR"
BASE COUNT          627 a          831 c          954 g          623 t
ORIGIN      
        1 agaacgcttc tatgacgcag caaggacgcg agttcgacat gtcgcagtat cgcatcgaga
       61 aagaacccta ttacgccgac gttcggggtg aagtggatct ctttcttact gcggccaaga
      121 gccggatgcc ggtgatgctc aagggaccca ccgggtgcgg gaaaacgcgc ttcgtacaat
      181 acatggcgta tcgcctcggc cggcccctga tcaccgtggc ctgccacgaa gacttgacgg
      241 cgtcggacct cgtcggtcgg tacttgctca aaggtcagga aacggtctgg gtcgacggac
      301 cactgacatt gggtgtcaag cagggcgcga ttgtctacct cgatgaggtg gtcgaggcgc
      361 ggaaggatac gacggtcatt attcatccgc tcagcgacga tcgccggctc ctgccgatcg
      421 agaaaaaggg ccaggccatc gaagccgcag aaaatttcct cctcgtgatt tcctacaatc
      481 ccggttatca gaatgtgctc aaggatttga agcctagcac caaacagcga tttgtcgcga
      541 tcgagtttga ctaccccact gccgacatcg aagcctcaat cgtagaacgt gaggccggca
      601 tcgctcacga ccttgcggtc aggctcgtta agctaggcgg gaaaatacgc catttaaaga
      661 atcacggcct cgaggaaggc gtgagtacgc gactcctcat ttacgccggg acgctcatcc
      721 gcgagggtat tgcaatcgag cgggcctgcg acgtggcgat tgccagaccc attaccgacg
      781 atgccgacat gcagcgcagc atcatggaac tcgtcaaggc catgttctga acggtatgcg
      841 aatgtgttgc ccgcgctccg caaaaacctc aactgcagtc accaagggaa tgcggaaccg
      901 ttggtccgca gccttctagt cagtgccgga cgtctcgcca tcgatcgtac acccatgtgt
      961 gaaagactgg cagggtggta tcctgcttac cgtcttcgct cagcccaagg cttcccggac
     1021 ggcctgggtt ggtctacgcg gcgatgcagt caaaattcgc atcgcggcat ctcccgtcga
     1081 cggagcagca aaccgcgagt tgatccgttt cctcgctgag gagttgtcga tgcctatgag
     1141 cgctatcgac attcactctg gtgccggtgg aaggcacaaa cgcgtctggg taaggggaac
     1201 gacagcggtg gacgtcacgg cgtatctgat acggaaatgt cagggataag gatgaacctg
     1261 ttcagagcct tggtgcttat ggtcgggacg ttgttgtgtg cctgttcagg accgaaaccg
     1321 gtcctgtatc cgaacgcaca cttgcaatcg gtggggaacg agacggcgga gcaggacatc
     1381 gaagcatgta aagaagcggc tgaaaccgcc ggtgcggatg aaggcagcgg caaggccgga
     1441 cgtgtcgcga caagtacggg ggttggcgcc ggcgttggtg cagccagtgg agcggttgga
     1501 ggtgcgatct ccggagcggc cggtcaagga tccctgattg gagcagccag tggagcggtg
     1561 tggggtttat tgacgggtct cttcactgcc ggcagctctc aaccgagcca ggccttcacg
     1621 aattatgtga atcggtgttt gcaggaacgg ggatatgagg tgaccggttg gcaataacgg
     1681 cgagcaggtt gggcaatgca catgaaacag tcattcgtga cgtgtccaaa aaatcctccg
     1741 cctatccggg agatgacagg atgccgaacc ggtgaacaag gtcctcatat catggcttgt
     1801 tattgcgctc gccggctgtt cgggacccga gccgatgctg cgggcaaacg ccaagttgct
     1861 gttgaaaggc cgcgaagctt cccatctgga agtcgccgcg tgccagcaga aggcagaagc
     1921 agccggccta cagcccggca ctgccgggag agcgggaaac atggctgcgg gtgcgggact
     1981 gggactgatc gccggtgcag cggtcggggc gtcgaccggg gtgatcgggg gtgcaacggg
     2041 cgtggccata ggcgcagctg cgggcgccgg ccttgggctc atcatcggat tggccggcgg
     2101 ggcctaccga ccgctagaac ccgatccacc ttatgctgat gccgtggtgc gatgtttgat
     2161 cgagcaggga tatgaagtca cggggtggca ctaattcaat ccgttgtgtc cggcgaggcg
     2221 tccgaggctt tatggcgtag ggcccgaccc ttgtccggcg tgggatcggt aatcacacca
     2281 tacacttccc gtccttcgtg accttccgga agatattccg tgatcggcat tccgtcttcc
     2341 cggacaaaca ataagcagtg gcaatatttg taaatctgca tttcgtcgca ggcgcagatc
     2401 catcggcgtc gtttggcctc ggcggcttta tctttgtaaa aattgcaggg gcacagcggc
     2461 tttcccagct cgtcaacgtg catggccagg cctttgacca cggactccgt gacggcccga
     2521 ttggggtgca tcgtggtgcc gctcttcgcg gcgaagctat ttacgtactt ccacattttg
     2581 tccaggctgg cttgtttggg ttctgccatc gtcagttgtc cattggcggt gatgtcggct
     2641 cgaggcgata tcagcgtctg gcgccagttt acaagcgttg ggcggtcctt tacaatccgc
     2701 aacttcgccc catgttgcgc aaattgctgg gaattccccg aggctcgctt gcgagttgac
     2761 tcctaaacgc gacaggtcga acaccctcct gtatggacga cgggactaac cgagacggct
     2821 gagaaggatg acgagcggtg gagtctgcga accttcaatt gatcaaccga ttggccggtg
     2881 agcttgacgc tgtcgaggcc gctgcactcg tgccacggat cgtggcagct gcgcacaaac
     2941 cagcagcgat cctggccttg ctggatgaac ttgaggccat atccgccaag gcggtccgag
     3001 ccgccatcga agcgctgcct gaattggacc ggcgt
//
LOCUS       DASTQY010000457         5336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_93667, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000457 DASTQY010000000
VERSION     DASTQY010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93667"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VFR82_14690"
     CDS             complement(<1..524)
                     /locus_tag="VFR82_14690"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEU4502896.1"
                     /translation="MSSELDRQGLLDIFIMEAAEALAALSAAVNPPGDAVPSAQELQD
                     QYVWAHKVRGASGIYGFTGLSLLGELLESTLEQSTSIDSALWLKTVGALRGMVEAFQS
                     QLDVIKQGGQEDPSVSAHWKKEVMTLLPVSSNVSSESGSEELGTDYLVPTIDAEVLSY
                     FAPEADEYLQTLEVL"
     gene            complement(576..2450)
                     /locus_tag="VFR82_14695"
     CDS             complement(576..2450)
                     /locus_tag="VFR82_14695"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU4502897.1"
                     /translation="MLRLNFLKWFENLKTQAKLLTVFGLVGVIIAAMAGVGIVTNGRL
                     ATQAETVYVDYTVPLTEFNQLLFNVNRYHEALVDVSRTPRFSDFEIELKDIAPYRAEV
                     ERLLTSYEKTTLRTSSTGRDEAKDLAELKAAVTQFFNEGDAAVTTIKESFENKALTSS
                     QALAMRELGALAITVSMSPAYEKVTQRHAEQIKTMSEVAKDLSDEAKGMAANATIILV
                     VGGIAAVLLGLALGYWVAHKLSLGIGQVASVAQQAASGNYQARAKIASKDELGQMATS
                     FNTMLDRITALVTSEGERDEMQKRLMNFLVLVSDVGKGDLTKRGEVTADMFGNLADGF
                     NLMIARFGQLLKQVREAAERVNKSAGTLRDSAGQMAGTAKHQADESAKTLSAVEQLAS
                     QMRQVAETAGASSESAKQVLQATERGRVAVQETVQDMQSIRSAVQRMSKQVKGLGDRS
                     LEISQIVSTIRDIANQTNLLALNAAIEAAGAGEAGARFAVVADQVRKLAESSTQATRE
                     IADLVKVIQSETQDAVVAMEQETQAVEAGSASALRTGEVFNEISGIAQRSAELAQTIA
                     SSAATQTASTDQVGRSIKDFTGGAVATQKATDAARLTVEDMAKLADGLTASVAQFKLA
                     "
     gene            complement(2522..3043)
                     /locus_tag="VFR82_14700"
     CDS             complement(2522..3043)
                     /locus_tag="VFR82_14700"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEU4502898.1"
                     /translation="MSAPQGATSVATSHADAIVASQGSAATTSGTSTIRAAIVSLGGE
                     LFTIDLQNVREVFVVESVTPVPGMPPGLIGVTNLRGSVVPLLDLRQMIGVTAETALKY
                     AVVVKHGNWQVGVLVDTVPEIRTLSKDQFLPAPIGTGDGSNPFVSTVVKLEDRLRGVL
                     ETSAVLSHFEGTG"
     gene            complement(3049..3411)
                     /locus_tag="VFR82_14705"
     CDS             complement(3049..3411)
                     /locus_tag="VFR82_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4502899.1"
                     /translation="MSKIVVVDDSYAELQVIEGVLKAANHTVVSFPNTEKLEDKVVGE
                     KPDLIVMDVVMPGRNGFQACRDLKNDDRTKNIPIILCTSKGNESDKFWGQQQGANAHV
                     VKPFKGDELLAAVKRVLG"
     gene            complement(3458..4195)
                     /locus_tag="VFR82_14710"
     CDS             complement(3458..4195)
                     /locus_tag="VFR82_14710"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEU4502900.1"
                     /translation="MKATWFNWRMAGGIAAVALMFNTSAVLAAGEAEVAEHLIDLVKI
                     GRGVMSEQMANINDASKTNKGFTGDYMAAQVIERFKKKTKIDLRIPNAVPQAGLYLAL
                     VEAEKDVVDEAQPVINRAGIGFKGFIPAVFARKTGENFYKKTGVKLKLTGIDYRNVNN
                     RPDDFEAEVLRMFSDPRHPKGQTYVRNTMLDGRPVLRMMDPEYAAASCLSCHGAPKGG
                     KDITGGHKEGWKEGDLAGAISVVLPLK"
     gene            complement(4644..>5336)
                     /locus_tag="VFR82_14715"
     CDS             complement(4644..>5336)
                     /locus_tag="VFR82_14715"
                     /inference="COORDINATES: protein motif:HMM:NF014060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HEU4502901.1"
                     /translation="RVRGNTLVIDLAKRYRIVSSAPRGGGIVRGRSILNHQVPANPVM
                     PGGRSVTEWSDPARYLGQLARRVNAAMPVVGLMTAVPMTQLVVDREQSGSLLLECFCT
                     VGVTNAVRAGAPVRSRLDRKQDRNQAGTINIILVTNATLTVPALIGAVQVATESKTAT
                     LIKHKVPSAYERALATGTGTDAVVLASSLAGRHKLQYSGTHTEMGAMIGRLVARCVER
                     GLHRFARWSKAR"
BASE COUNT         1031 a         1643 c         1464 g         1198 t
ORIGIN      
        1 agaacctcca aggtctggag atattcgtcg gcttcagggg caaaatagga aagcacttct
       61 gcatcgatgg tcgggaccag gtagtctgtg cccagctctt ccgaaccact ctccgaggag
      121 acatttgagg aaaccggcaa cagagtcatg acctctttct tccaatgggc actcacggac
      181 ggatcttcct ggccgccctg cttgatgaca tccagttgcg attgaaaggc ttcaaccatg
      241 ccgcgcaatg caccgaccgt cttgagccag agagccgagt cgatggacgt ggactgttcc
      301 agtgtcgatt cgaggagctc gcccaaaagt gagaggcccg taaacccgta gatgccggaa
      361 gcgccacgaa ctttgtgcgc ccatacatac tgatcttgga gttcttgggc gctcggcacc
      421 gcgtcgccgg gaggattcac ggccgccgag agcgcagcga gcgcttctgc ggcttccatt
      481 ataaagatgt cgaggaggcc ctgacggtcc agttcggagc tcatccctat cctttctgac
      541 ccggagcgcg ggtacggcgc tccgggtacc agaccttagg ccagcttgaa ctgcgcgacc
      601 gaagccgtca gaccgtcggc gagtttggcc atgtcttcga cggtcaaacg tgccgcatcg
      661 gtggctttct gcgtcgccac cgcaccgccg gtgaagtcct tgatcgagcg acccacctgg
      721 tccgtagaag ccgtttgtgt ggcagcagaa ctggcgatgg tctgggccaa ttcggcagac
      781 cgttgggcga tacctgaaat ctcgttgaac acctcaccag tacgcagcgc ggaagccgat
      841 ccggcttcca ccgcctgggt ttcctgttcc atggcgacga ccgcgtcctg tgtttccgac
      901 tgaatcactt tcacgagatc cgcgatttcg cgggtcgctt gcgtggagct ttcagccagt
      961 ttccggacct ggtccgcgac gaccgcgaac cgtgctccgg cttctccggc gccagccgcc
     1021 tcgatggcgg cgttgagcgc cagcaggttg gtctgattcg caatgtctcg aatcgtcgac
     1081 acgatctgtg aaatttccaa cgaccggtcg ccgagtccct tcacctgctt cgacatgcgt
     1141 tgcaccgctg aacgaatgct ctgcatgtcc tgcacggtct cctgcaccgc gacacgcccg
     1201 cgctccgtgg cttgcaacac ttgtttggcg gactcggatg aggcgcctgc cgtttcggca
     1261 acctgacgca tttgcgaggc gagctgctcg accgcgctca gcgtcttggc cgactcgtcc
     1321 gcttggtgtt tggcggttcc ggccatctga ccggcgctgt cgcgaagcgt accggctgac
     1381 ttgttgacac gttcggccgc ctcacggacc tgtttgagca gctgtccgaa ccgggcgatc
     1441 atgaggttga atccgtccgc caggttgccg aacatgtcgg ccgtcacttc accgcgcttc
     1501 gtcaggtctc ctttaccgac atccgacacc aagacaagga agttcatcaa acgcttttgc
     1561 atctcatcgc gttcgccttc cgacgtcacc agcgctgtaa tacggtccaa catggtgttg
     1621 aacgaggtgg ccatctggcc caattcgtcc ttcgacgcga tcttcgcgcg ggcttggtaa
     1681 ttgccgctcg ccgcttgctg cgcgacgctg gcgacctgtc cgatacccag tgagagcttg
     1741 tgtgccaccc aatatccgag tgccaagccc aacagcacgg cggcgatacc accgaccacc
     1801 aggattatgg tggcgttggc agccatgccc ttggcttcgt ccgagaggtc cttcgccact
     1861 tccgacatgg tcttgatctg ttcggcgtgg cgttgcgtca ccttttcata ggcggggctc
     1921 atactcaccg tgatggctag agcgcccaat tcccgcatgg ccagggcctg ggatgatgtc
     1981 agcgccttgt tctcgaaact ttccttgatg gttgtgactg cggcatcgcc ctcattgaag
     2041 aactgggtga ccgcggcctt gagttcggcc aagtctttgg cttcatctcg gccggtgctg
     2101 gatgtccgta acgtcgtctt ttcatagctc gtgagcagcc gttcgacttc ggcacggtac
     2161 ggggcaatgt ccttcaactc gatttcgaag tctgagaagc gcggcgtgcg ggagacgtcg
     2221 accaacgctt cgtggtaccg gttgacgttg aacaggagct ggttgaattc ggtcaacggc
     2281 accgtgtagt cgacgtacac ggtttccgct tgggtcgcca agcggccgtt ggtcacgata
     2341 ccgacgcccg ccattgcggc gatgatgacg ccgacaaggc cgaacaccgt cagcagtttg
     2401 gcctgtgtct ttaagttttc aaaccatttc agaaagttga gcctcaacat gggaacctcc
     2461 tccttctcct agcgatccga tcgcaccatg agcatcacac cctgtcctga tgatcttccg
     2521 actatcctgt gccttcgaaa tgagaaagta cggccgatgt ctccaataca ccgcgaagcc
     2581 ggtcttccag cttcacgacg gtcgacacga agggattgct cccgtctccc gtgccgatcg
     2641 gagccggtaa gaactgatcc ttcgacaagg tgcgaatttc gggaaccgta tccaccaaga
     2701 caccgacctg ccagttgccg tgcttgacga ccacggcata cttgagagcg gtctcagcgg
     2761 taacgccgat catctgcctg aggtcgagca aggggacgac ggatcctcgc aggttcgtga
     2821 caccgatcaa tcctggcggc atgcccggaa ccggcgtcac cgattcgacc acaaacactt
     2881 cccgcacgtt ttgcaggtcg atcgtgaaca attccccccc taaagagacg atggccgccc
     2941 gtatggtcga tgtgccggaa gttgtggcag cactcccttg cgaagcaacg atggcatcgg
     3001 cgtgggaggt cgcgaccgag gtggcgccct gcggagcact catggcggtt atcccaacac
     3061 ccgcttcacg gccgccagta attcgtcacc cttgaacggc ttgaccacgt gggcattggc
     3121 accctgctgc tgaccccaga atttgtcact ttcgttgcct ttggaggtac agaggatgat
     3181 ggggatattt ttcgtccggt cgtcgttctt gagatcgcgg caggcctgaa agccgttgcg
     3241 gcccggcatg acgacgtcca taacgatcag atccggcttc tcaccaacca ccttgtcttc
     3301 gagcttctca gtgttgggga aggacaccac ggtatggttc gccgctttca acactccctc
     3361 aatcacctgt agctcggcgt acgaatcgtc gacgacaaca atcttgctca tcggttctct
     3421 ccttgttctg gttatgaatg acgtccggat tccaccacta cttcaggggc agcacgacgc
     3481 tgatcgctcc ggccaaatcc ccctctttcc aaccctcttt atgaccaccg gtaatgtctt
     3541 tcccgccctt gggcgcgccg tggcatgaca aacaggacgc cgccgcgtac tcaggatcca
     3601 tcatgcgcag caccggccgg ccatccagca tcgtattgcg gacgtacgtt tggcccttgg
     3661 gatggcgcgg atcactgaac atccgaagca cttcggcctc gaaatcatcc ggtctgttgt
     3721 tcacgttgcg ataatcaatt ccggtcagtt tcagcttcac ccctgtcttt ttgtaaaaat
     3781 tttctccggt cttccgcgcg aatacggcag ggatgaatcc tttgaagccg atgccggcac
     3841 gattgattac cggttgggct tcatcgacaa catctttttc cgcctcgacg agcgcgagat
     3901 aaagacccgc ttgcgggacg gcgttgggaa ttctgaggtc gatcttggtc ttctttttga
     3961 accgctcgat gacctgagcg gccatgtagt cgccggtgaa gcccttgttg gtcttggagg
     4021 cgtcgttgat attcgccatc tgctccgaca tgactccacg gccgatcttg accagatcga
     4081 tgagatgttc cgccacttcg gcctcgccgg ccgccaggac cgcgctcgta ttaaacatca
     4141 gcgccaccgc cgcgattccg cccgccatcc gccaattgaa ccatgtcgcc ttcataccca
     4201 tcctccttcg tgaagtcact cgaccggatg cttcgtttct tttctcactc ttcgcgcgca
     4261 gcacctagca attcctgttt ttcaacacga gatgtcgccg atgcacccgc gcatgacgcc
     4321 gcggcggacc gctcgaagct tcggctcact gtgcaaaggt tatgcttatt cgcttacaag
     4381 cagcgtcggc gtgaatacag aacgcaaaca cctgacggat cagagagtta cgtactctac
     4441 acatgagcag cattcatact cgtccgccgc acgattgttc aggttgcgac aaaatgatgc
     4501 cgtttgtgca aaaattgacc actcgcctgc ttgtgttatg gcagcagctt cgcctgaagg
     4561 ccgaggcgga ggttctgaat gggactttcg cttagggaaa atcgaagctg aggaaggaac
     4621 gctcaaagaa ggggctttac tatttatcgg gccttgctcc aacgagcgaa gcgatggagc
     4681 cctctttcaa cgcaacgggc gacgagccgc ccaatcatcg ctcccatctc ggtatgcgtc
     4741 ccgctgtact gcaacttatg acggcctgcc aagctgctgg caagcaccac cgcatcggta
     4801 ccggtccctg tcgcgagggc ccgttcataa gcactcggga ccttatgttt gatgagtgtt
     4861 gcggtcttgc tctctgtcgc cacttgcact gctccgatca aagcaggaac cgtcagagtt
     4921 gcgttcgtaa cgaggataat attgatcgtt cctgcctgat tgcggtcctg ctttctgtcc
     4981 aacctggacc gaaccggtgc gcctgcccgg acagcgttgg tgacgccaac ggtacaaaaa
     5041 cattccaaca gtagcgagcc tgattgttca cggtcgacga ctagctgtgt catgggcaca
     5101 gccgtcatga ggccgacaac cggcatggcg gcgttcactc tccgtgccag ctgccccaaa
     5161 tacctcgccg gatcagacca ttccgtgacc gaccgaccgc ctggcatgac agggttggcc
     5221 gggacttggt gattgaggat cgaccgcccc cgaacgatgc cccctccgcg cggcgcagaa
     5281 gagacgattc gatacctctt cgcaagatca atgaccaagg tattcccacg gactcg
//
LOCUS       DASTQY010000458         4504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1115749, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000458 DASTQY010000000
VERSION     DASTQY010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115749"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VFR82_14720"
     CDS             <1..621
                     /locus_tag="VFR82_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEU4502902.1"
                     /translation="GESTIVPFYGSEKRMAPVESYVRVSDEAIYEIGEITFPHIIIIF
                     HPQVITHGKSYTMPFYFGLKEDGIALINNDGPMKLHKDQARELEERRAKLYYFPATKL
                     SLEVAGMDLATNMALMGCIGAITGLTNMVGLEQAVKDRFLGKGFVVSGGTAALDSVVE
                     RKFKKKQELIDKNVAVMRAGWNYAVDHGWASADVKRAEEPAAAATA"
     gene            631..954
                     /locus_tag="VFR82_14725"
     CDS             631..954
                     /locus_tag="VFR82_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HEU4502903.1"
                     /translation="MYLVADINVEICAAKSCKLCTQYCPEANTILYSDEMGKDKGFKY
                     GSAYVAVDRCKGCAQCVWVCDNMAKNNAIKMIMIDQLPKAALTDNVTYGEKSTTAVLA
                     SPVVG"
     gene            980..1747
                     /locus_tag="VFR82_14730"
     CDS             980..1747
                     /locus_tag="VFR82_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HEU4502904.1"
                     /translation="MATTAQDTREKIIVPGPAGFHPPSAAQLGVSLPDPGQGLFYGLL
                     EPNEEKVIEEMARKMLTSPNATIFPGPLLLWAWNDHAVEKAKATLEIAAQIPEVMIIP
                     MPDYRPKYPKIDPEEVINPNHPNLTIWGNKIEACIFIGVHCHYANLTLKMIRAGTNCC
                     TMAICAEQGHEDAMLTIRDSDTAKIKRVAQIFKKVREEMGIKLPENGENVRFTGTQAK
                     VHGGKTHTNPVTFAPSPSGTGSAALFGHSAENMKREG"
     gene            1880..3223
                     /locus_tag="VFR82_14735"
     CDS             1880..3223
                     /locus_tag="VFR82_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU4502905.1"
                     /translation="MSEALEAQQKTNPQGDPITSVAAPKADGKKDPHFEAKRQKIVTP
                     EYMFHEAPRTKEFITGSEAAKEAIRRSNVDLAVAYPITPQSETMQLVGVLYGEGYVKE
                     YYRGEEEVGVMAAIAGGSRSGVRCFTATAGPGTLRGMEGIASWPGHRLPAVAMFTCRV
                     VNAPLAIQPDNIEVAYLLNCGMIVFHAENQQDMFDFIMAGFTISEKNDVTLPVGVCCD
                     GFFVTHARGYVRMQDRGIKLPPREAWRGAVPVLDAENPPARLSRDAPVQKSNFMAYNI
                     HAVWQQEVWAAVERSRKYISRYMGGMLTAENVEGADAVIIASGSAAAQSREAVRLCKE
                     KGLKVGLIKVRSLRPFPTLELRQLCGNVKLIVVPEFNYVGWLAKEVATAIYGYSKAKI
                     IGGPRVYGGQSMPVELIVDEVESGLTGKKSTNVAMSSIMGGAVNPDEVAHFMRSI"
     gene            complement(3301..3549)
                     /locus_tag="VFR82_14740"
     CDS             complement(3301..3549)
                     /locus_tag="VFR82_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502906.1"
                     /translation="MTDPIVQAYKDVETAVERYTKLLQDHVLMLQNTEAPGSDKVVRM
                     TQGSKAMRDSAMIYLSYAKYVAYGMPASEEMVEDEIQG"
     gene            3587..>4504
                     /locus_tag="VFR82_14745"
     CDS             3587..>4504
                     /locus_tag="VFR82_14745"
                     /inference="COORDINATES: protein motif:HMM:NF020804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HEU4502907.1"
                     /translation="MTAFQAKGAARLSPVLSRILEAIEPEQSDAGTALAVAELSRLFT
                     KEREALGMAYLDDPSLARAYLKYFFPVNLSKIQVLLDELPEDWHKAAGNRPVRVLDLG
                     TGPGTGALAVLDWLYQHDLDWLKQLTVVAVDASADALRQARHVWDAYERQANTSDARL
                     ILYEGDLGRPTSGWRDHAVQRGPYDLIIVANCLNELFGRTDHSLKKRSGLMGDLLRLL
                     APHGTLMLLEPALRETSRALHQVRDIILLEKRCTVYSPCLHEGNCPALANPNDWCHEE
                     RAWEPPASIQRIDHEVGFIKDALKFSYLLL"
BASE COUNT         1033 a         1239 c         1292 g          940 t
ORIGIN      
        1 ggcgaaagca cgatcgtccc gttctatgga tcggaaaagc ggatggcgcc cgtcgaaagc
       61 tacgtccgcg tctcggacga agcgatttac gaaatcggag agatcacctt cccgcacatc
      121 atcatcattt tccatcccca agtcattacg cacggcaaat cctataccat gcccttctac
      181 ttcgggttga aagaagatgg cattgcgttg atcaacaacg acgggccgat gaagctgcac
      241 aaagatcaag cccgcgagtt ggaagagcgc cgtgcaaaac tttactactt tccagcgacg
      301 aagctttcgt tggaagtggc agggatggac ctcgcgacca acatggcgct gatgggctgc
      361 atcggtgcga tcacggggct gacgaacatg gtggggttgg agcaggcagt gaaggacaga
      421 ttccttggca agggattcgt cgtatctggc ggcaccgccg cgctagatag tgtcgtggag
      481 cgaaagttca agaagaagca agaattgatt gataagaatg tcgccgtgat gcgggcgggg
      541 tggaactacg ccgtcgatca tggttgggcg tcggcagatg tcaaacgggc agaagagcct
      601 gcagccgctg ccaccgcgta aaggagcacc atgtatcttg tcgctgatat caatgtcgaa
      661 atttgcgctg ccaagagctg caagctctgc acgcagtact gtccggaagc caacacgatt
      721 ctttatagcg atgagatggg gaaggataag ggcttcaagt acgggtccgc ctatgtcgcc
      781 gtcgaccgct gtaagggttg tgctcaatgc gtgtgggtct gcgataatat ggccaaaaac
      841 aacgcaatta aaatgatcat gatcgatcag ctgcccaagg cggcactgac cgacaatgtg
      901 acctacggtg agaagagcac cactgcggtg ctcgcgagcc cggttgtcgg atagcgtcga
      961 gtccagaaag gggaacggag tggccactac agcgcaagac acgagggaaa aaatcatcgt
     1021 tcccggaccg gcagggttcc atccgccgtc ggcggcccaa ctcggtgttt cgttgccgga
     1081 tccaggtcag ggcctgttct atgggcttct tgagccgaac gaagaaaaag tcatcgaaga
     1141 gatggcccgt aagatgttga ccagtccgaa tgccaccatc ttccccggtc cactgctcct
     1201 ctgggcgtgg aacgaccacg ctgtcgaaaa ggccaaggcg acgctcgaga tcgcggcgca
     1261 gattcctgaa gtcatgatca ttcccatgcc ggattacaga cccaagtatc caaaaatcga
     1321 ccccgaagaa gtcatcaacc cgaaccatcc caatctgacc atttggggca ataagatcga
     1381 ggcctgtatt tttattggcg tgcactgcca ctatgccaat ttaacgctga agatgattcg
     1441 cgctggaacc aactgctgca cgatggccat ctgcgccgag caaggccatg aggatgcgat
     1501 gctgacgatt cgcgattccg ataccgccaa gatcaagcgg gtcgcgcaga tttttaagaa
     1561 ggtgcgggag gagatgggta tcaagcttcc cgaaaacgga gaaaatgtcc gttttacagg
     1621 gactcaggca aaagtgcacg gcgggaaaac gcataccaat cccgtgacat ttgctccatc
     1681 tccgagcgga actggaagtg ccgcgctgtt cggtcattct gcagaaaaca tgaaacgcga
     1741 aggctagtcg cgaacgttcc tcagggttcg agtgacaacg catagcgatg caggatgttc
     1801 aaaaaggccg ttcagccctt tgcacaatgc gagaatgctg tggggggctt tttcaacatg
     1861 ctgttaatga ggtgctatca tgagtgaagc tctcgaggct caacaaaaga ccaaccccca
     1921 gggcgacccg attaccagcg tcgcagctcc aaaagcggat gggaaaaaag atccgcattt
     1981 cgaggccaag cggcaaaaaa tcgtgacgcc ggaatacatg ttccatgaag cgccccgcac
     2041 gaaggaattc attaccggta gcgaagcggc caaagaagcc attcgccgct caaacgtgga
     2101 cttggcggtc gcctatccca ttacgccgca gagcgagacg atgcagttgg tcggcgtgct
     2161 ctatggtgaa ggctatgtca aagaatatta ccgcggagaa gaagaggtcg gcgtgatggc
     2221 tgcgattgcc ggtggatcgc gatccggcgt gcggtgtttc acggccacgg ccggtcccgg
     2281 tacgttgaga ggcatggaag ggattgcgtc atggcctggc catcggttgc ctgccgtggc
     2341 catgttcacc tgccgtgtcg tcaatgcgcc gttggccatt caacccgaca acatcgaagt
     2401 agcctatttg ctgaattgtg gcatgatcgt gtttcatgca gagaatcagc aggacatgtt
     2461 cgatttcatc atggccggct ttacgatcag cgaaaagaac gatgtgacgc tgccggtcgg
     2521 cgtatgctgc gacggcttct tcgtcactca cgcgcgcggc tacgtgcgca tgcaggaccg
     2581 cggtatcaag ctccccccac gcgaagcctg gcgaggagcc gttcctgtgc tcgatgcgga
     2641 gaatcccccc gcccgactct cgcgcgacgc cccggtgcaa aaatccaact ttatggccta
     2701 caacattcat gcggtgtggc agcaggaagt gtgggcggct gtggagcgtt cccgcaaata
     2761 cattagtcga tacatgggcg gtatgctgac cgcggaaaac gtggaaggcg ccgacgccgt
     2821 catcatcgcg tccggcagcg cggccgcgca atcccgcgaa gcggttcgcc tttgcaagga
     2881 aaaaggcctc aaggtcggat tgatcaaggt ccgctcgcta cgccccttcc cgacgctgga
     2941 gcttcgtcag ctctgtggaa atgtcaaact catcgtcgtg cccgagttca actacgtcgg
     3001 atggttggca aaagaagtgg ctaccgcgat ttacggatac tcaaaggcca agatcatcgg
     3061 cggcccgcgt gtctacggcg gccagtcgat gccggtggaa ttgattgtag acgaggtcga
     3121 atccggtctc accgggaaga aatccaccaa cgtcgcgatg tcctcgatca tgggcggtgc
     3181 cgtcaatcca gatgaagtcg ctcatttcat gcgcagcatc tgagatttcg ggctgaaaac
     3241 gaagagctcg tcaggaaggc attctgacga gctcttttcg tttctctttc gaaccgcagc
     3301 ctatccttga atttcgtctt ccaccatttc ctcgctggcc ggcatgccat aggcgacgta
     3361 tttggcatag gacagataaa tcatcgcgct gtcccgcatg gctttagatc cctgagtcat
     3421 gcgtacgacc ttatcggaac cgggtgcctc cgtgttttgg agcatcagga catggtcctg
     3481 cagcaacttg gtatagcgct ctacggccgt ttcaacatcc ttgtaggctt gcacaatcgg
     3541 gtcggtcata gttgtcctcc ctcgcaagct cagcatacac taggggatga cggctttcca
     3601 agccaaggga gcggcccggc tctcacctgt cctgtcacga attcttgagg ccattgaacc
     3661 ggagcagtcc gatgccggga cggccctcgc cgtcgccgaa ctctcgcgtc ttttcacgaa
     3721 agagcgtgaa gcgctgggca tggcctatct cgacgatcct tctctggccc gggcgtatct
     3781 caaatacttt ttccctgtca atctttccaa aatccaagtc ctgcttgacg aactgccgga
     3841 ggactggcac aaagctgcag ggaatcggcc ggttcgagta ctcgatctcg gaaccggacc
     3901 aggcacgggt gccctggcgg tcctcgactg gctttaccag catgatcttg attggctcaa
     3961 gcaactgacc gtcgttgccg tcgatgcctc cgcggatgcc ttgcgccagg cccgacatgt
     4021 gtgggatgcc tatgagcgcc aggcgaatac cagtgatgcg aggctgatcc tctatgaagg
     4081 tgatctcggg cgtccaacga gcggctggcg ggatcatgcc gttcaaagag ggccctacga
     4141 tctgattatc gtcgcaaact gcctcaacga gctgttcggc cgaacagacc attccctaaa
     4201 gaagagatcc gggctaatgg gtgacctgct tcggctgctc gcgccccatg gcactctgat
     4261 gctcctcgag cccgccctac gcgaaacgtc acgggctttg catcaagtcc gtgatatcat
     4321 acttctcgag aagcggtgca cggtctatag tccatgcctc catgagggga attgtcccgc
     4381 cctggccaac cctaacgatt ggtgtcacga ggagcgtgcc tgggagcccc cagcttctat
     4441 ccagcggatc gatcacgaag tggggtttat caaagacgcc ctgaaattct cctatctgct
     4501 gttg
//
LOCUS       DASTQY010000459         7998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1317469, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000459 DASTQY010000000
VERSION     DASTQY010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317469"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFR82_14750"
     CDS             <1..261
                     /locus_tag="VFR82_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502908.1"
                     /translation="VLSFRNATQKAQLEEDLAWLETRRIPLKRELLDLFVSRTTYEEQ
                     TFTFCVDGVQRRYQALDTNRFTRLSDGPGPCEPMEFSSLRKT"
     gene            complement(366..761)
                     /locus_tag="VFR82_14755"
     CDS             complement(366..761)
                     /locus_tag="VFR82_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502909.1"
                     /translation="MKSVTRGLCFAVFGIALLCSTDTFAMDQPDTQSPVMLAQAGGGS
                     GGGSGGGTGGGTGGGMGSGGSGMGSGQSGSGGSGSGGSGMSGSSSGSSGSDTMQGGSS
                     GSSSSGMGSGKTGPQSSGKQGAKSGQSSR"
     gene            complement(821..1516)
                     /locus_tag="VFR82_14760"
     CDS             complement(821..1516)
                     /locus_tag="VFR82_14760"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU4502910.1"
                     /translation="MHTTFSVIGGVGFGAGLMFILDPDRGRRRQALMRDKRVRLSRKT
                     REFAGSTSRDVRNRAKGLGAVLKSWIEPASPVPDHIVAERVRSKLGMYSRHPSAIDVH
                     VTDGVVTLSGPVLKDEMDSICSAMARVPGVMRISNLLEPHESPDDVPALQGPIDPRPG
                     PRFALMQSHWSPAARLAAALMGTVALLYGLSQRTTGAATLAATGLGLLVRSATNQEFA
                     RLFYFTEQFKKTV"
     gene            complement(1788..2342)
                     /locus_tag="VFR82_14765"
     CDS             complement(1788..2342)
                     /locus_tag="VFR82_14765"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEU4502911.1"
                     /translation="MDLQIEGVHTDVTPQWRADIEARVADLHPGHDITHVRVRLTGHD
                     HRSPDDSHSVLIVVQIPGHTITAEKQQGTFEEAIHDTFDAVAVELEKIREKRASHEVD
                     VSAPPERGIVTKLFPSESYGFIALEDGTEVYFHRNAVRDAAFEQMDGMEVSLNIEPGE
                     KGPQATVVQPLPPEAYYVDKRSAA"
     gene            complement(2506..3357)
                     /locus_tag="VFR82_14770"
     CDS             complement(2506..3357)
                     /locus_tag="VFR82_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEU4502912.1"
                     /translation="MASEVGKVASLWRYPVKSMQGEELSESEVTQHGLLGDRVYALVD
                     VAEGKAASAKNPVKWPTLFGCKARFAEQPKKGAAPPAVKITLYDGSTTSSPEGDCHQI
                     LSKALKRTVILAVADRGWMSGVHATLPPTWTGKAEQYWPDVDGLDRRDTVTDFTLPRG
                     TFFDGATVHLITIATLIKLHQVYPKGRFEPQRFRPNILVATPEDMQGFVEQSWIGKTV
                     KIGELQLAITGSTGRCVMTTLAQGDLPKDNGILRTAVQQNEGNVGVYAQVVQGGTVKR
                     GDRVQVI"
     gene            complement(3495..4892)
                     /locus_tag="VFR82_14775"
     CDS             complement(3495..4892)
                     /locus_tag="VFR82_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEU4502913.1"
                     /translation="MTIPDQSQQPAASSDDHAPSSAFGLPLYTQVLIAVICGTAVGAF
                     LGQEPYLGGLRNEHLGRAGMFVILLLKTLAIPLIFFAVLDAFVSTILPLRQGTRLLVI
                     CLINVSVAMTIGLVIMNAWQPGLSWSSRVDELLNLASGSTPAGAPLAGGGSGTPLEEI
                     ASYIPRTMLQPFTSSNVIGVVILALVLGALLRRLRGPEEAPRHSVEQLVRGIERIYRW
                     LVRVLWWIILLVPFAVFGVLASIVGRVGLGVFSAVWVFLAAILVGLLFHAFVYYPLVA
                     WLVGKKSPRIYVGKGADAIMTAVSCNSSLATVPVTLRCLERMNVSPQSARLAACVGTN
                     LNNDGVTLYEAMAALFLAQALGYELSMGHQLLIVVASIMAGIGVAGIPEAGLIVLPLV
                     LSAAGLPNAVIAAAIPLIMTVDWILARVRSGVNVMSDMLVAILLDELETQQPTEMQAI
                     SQPQCEPTKPGIVPE"
     gene            complement(4933..5009)
                     /locus_tag="VFR82_14780"
     tRNA            complement(4933..5009)
                     /locus_tag="VFR82_14780"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4973..4975),aa:Pro,seq:cgg)
     gene            complement(5046..6149)
                     /gene="ald"
                     /locus_tag="VFR82_14785"
     CDS             complement(5046..6149)
                     /gene="ald"
                     /locus_tag="VFR82_14785"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU4502914.1"
                     /translation="MIIGVPKEIKDHEYRVSVTPDGVQALRQAGHEVLVEPSAGKGSG
                     YSDEQYRQAGASLAASKEGLFKEAALIVKVKEPQLSETGFFRPGQVLFTYLHLASSAE
                     LTKALLEANITAIAYETIEAKDGTLPMLKPMSEIAGRMSVQVGARYLEVTQGGRGLLL
                     AGVPGVEPAKVVILGAGVVGSAATRIAVGMGAQVTVINLDIEQLRDLDDLYQGRIVTR
                     ASTRAAIEESVLTADLLIGAVLIPGAKAPKLVPRALVARMKPGSVIVDVSVDQGGCVE
                     TSRPTTHSDPTYVVDGVLHYCVANMPGIVPRTSTQALTNATLPYLLTLSSFGVEAAVR
                     ADTGLAKGVNLARGQVTHRGVADAHGLRFAPLM"
     gene            6345..6731
                     /locus_tag="VFR82_14790"
     CDS             6345..6731
                     /locus_tag="VFR82_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502915.1"
                     /translation="MDAGGESCNACGAAGTALMKLSLGKDFFGRAYDRLSPSTDQSPK
                     WYCEQCSMQKNLQRDFRDIRAEFDKLTAGHTSELSKTETFQRAAIRLREIATILGGST
                     SGSSPFLAAPDVAQLIGRFQSTTMQA"
     gene            6746..7102
                     /locus_tag="VFR82_14795"
     CDS             6746..7102
                     /locus_tag="VFR82_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEU4502916.1"
                     /translation="MSSFKRHIFVCINERTPDDPRGSCSKLGSEALHARFKQEAKRLN
                     LKDIVRANKAGCLDHCALGPSVVIYPEGVWYTVRTESDVTEIMERHVIKGEVVTRLLM
                     PDHPAPTPLLPLSSLR"
     gene            7157..7522
                     /locus_tag="VFR82_14800"
     CDS             7157..7522
                     /locus_tag="VFR82_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502917.1"
                     /translation="MKQFPGLLGAWEALKGKTIEAVIPLKGKQGAAAIICNDGTFTVA
                     PPMMTEPYELGETLTAGRTLLEPKHRDAYVEYDRLAKQDKDALKQARLEKIIGAIENN
                     LEQIPELKARLKALVREWP"
     gene            7519..7818
                     /locus_tag="VFR82_14805"
     CDS             7519..7818
                     /locus_tag="VFR82_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502918.1"
                     /translation="MSSIPKKNMFEIFSQGLFEGVKPMMIVRDHLVRHPDRCTHHAVC
                     VPVCPTGAWLSTPPYKFDAARCLESCRLCLDACPSHAIFAVFRKGDKLLEPQQKQ"
BASE COUNT         1649 a         2444 c         2367 g         1538 t
ORIGIN      
        1 gtgctgagct ttaggaatgc gacccaaaag gcccaactcg aggaggatct ggcgtggctc
       61 gagaccagac gcatcccact gaaacgtgag ttattggatc tgttcgtttc tcggacaacc
      121 tacgaggaac agacgtttac attctgcgtc gacggcgttc agcgccgcta ccaggcactc
      181 gatacgaacc ggttcacccg cctgtcggat ggccccggcc cgtgcgagcc aatggagttc
      241 agtagcttga gaaaaaccta aagatgttag tgaacaaaga gacgtgaaga cagcgatttc
      301 acttagtact gctacgcggc gccggccagg tagaccggcg ccactaccgt ccgtcccaca
      361 ctgatttagc gactgctctg tcctgacttc gccccctgtt tgccgctgga ctgtggtccg
      421 gttttgcctg atcccatgcc ggagctcgaa ctccccgagc tgccgccttg cattgtgtcc
      481 gaaccgcttg atccgctcga gctaccgctc atccctgatc ctccactgcc tgaacctcca
      541 cttcccgatt gcccgctgcc catgccagat ccgccgctgc ccatgcctcc gccagttccg
      601 ccaccggtgc cgccgcctga tccgccgccg ctgccacctc cggcttgagc gagcattacc
      661 ggcgactgag tatcgggttg atccatggcg aatgtgtcgg tgctacagag caacgcgata
      721 ccgaacacag caaaacacaa tccacgtgtt acagatttca tgtgacaaac ctccacttgg
      781 ttgattgtga taccgacctc acgacgaatg aacggccccg ctacacggtt tttttaaatt
      841 gttcagtgaa atagaagaga cgcgcgaact cttgattggt agcgctgcgg accaacagtc
      901 caagaccggt ggccgccaac gttgccgcgc cggtcgttcg ctggctcagg ccatagagca
      961 atgccactgt gcccatcaaa gcggccgcca atcgcgcggc aggcgaccaa tgggattgca
     1021 tcaacgcaaa gcgggggccg ggcctcggat ctatcggtcc ttgaagagca ggaacgtcat
     1081 caggcgactc gtgcggctcc aagagattcg agattcgcat cactccggga actctcgcca
     1141 tggcactaca gatactgtcc atctcgtcct tcagtactgg cccgctgagc gtgacgacgc
     1201 cgtcggtgac gtgcacgtcg atcgccgacg gatggcggct gtacataccc agtttggagc
     1261 ggacccgttc ggccacaatg tgatcgggca ctggtgaagc cggttcgatc cacgacttga
     1321 gtacggctcc caggcccttc gcacgatttc gcacgtcgcg cgaggtgctg ccagcaaact
     1381 cccgggtctt tctcgacaag cggacgcgtt tatcccgcat caacgcctgg cgacgccggc
     1441 ctctgtctgg atccaggata aacatcagcc cggctccgaa gcccacgcct cctatcactg
     1501 agaatgtggt atgcatcgtt gttcctcctt cgctaaaatc ttcgttctct atgctccttc
     1561 caaggctcgc tcgttatgct ccttcccatt aggggtggat ggtggagatc gctgcgactt
     1621 gcgatgggtg cagacctgtc gctgcggcga tagcgagcaa tgcacgaagt tccgttcgga
     1681 cgaagtcctg gatggaacaa gtttgcgagc taagctgcga gcgggtcctg cggtttcacc
     1741 cctcgccgaa gcagcgggtc taccatccat ccgccacatt cccatcccta tgccgccgat
     1801 cgtttgtcga cgtaataggc ttcaggcgga agaggctgaa cgacggtggc ctgcggtcct
     1861 ttctcacccg gctcgatgtt gaggctgact tccataccgt ccatctgttc gaaggcggca
     1921 tcacgcaccg cattccgatg gaaatagact tcggtcccgt cttccaacgc aatgaatcca
     1981 tagctttcgc tgggaaacag cttcgtgaca atgccccgct cgggcggcgc gctgacatcg
     2041 acttcatggg acgcccgctt ctcgcgaatt ttctccagtt cgactgccac ggcatcgaac
     2101 gtgtcgtgaa tggcctcctc aaacgtgccc tgctgcttct cggccgtaat ggtgtgaccg
     2161 ggaatctgaa cgacgatcag cacgctgtgc gagtcgtccg gactgcggtg gtcatgcccg
     2221 gtgagcctga cgcggacgtg cgtgatgtca tgccccggat gcaggtcggc cacacgcgct
     2281 tcaatatccg cacgccattg cggcgtgacg tctgtgtgca ccccttcgat ttgtaggtcc
     2341 atcgttctcc tcgtcattgt cgttcgcggc gagtattcgt ccattaaggc accggtcttc
     2401 acatgaatga actagcgcca tccctggacg cagtggcgca gccgcgacag gaaacacttg
     2461 acgctgcgga agcgatcgat gagggggtga gaagaaggct gtagatcaga tgacttgcac
     2521 gcggtcgccg cgcttgaccg tgccgccctg aaccacctgc gcatagaccc cgacatttcc
     2581 ctcgttctgc tgcacagccg tgcggaggat gccgttgtct ttcggcaggt cgccctgggc
     2641 cagcgtcgtc atgacgcagc ggccggtaga gccggtgatg gcaagctgca gctcgccgat
     2701 cttgaccgtc ttgccgatcc aggactgttc gacgaagcct tgcatgtctt cgggggttgc
     2761 cacgaggatg ttcggacgga agcgctgggg ctcgaaacgg cctttcggat agacctggtg
     2821 cagcttgatc aaagtggcga tggtaatcag atggacagtg gcgccgtcga agaacgtgcc
     2881 acgcggcagc gtgaagtcgg tcacggtgtc tcgtcggtcc agtccatcca catcgggcca
     2941 atactgctcg gccttgccgg tccaggtggg tggaagcgtg gcgtgaactc cgctcatcca
     3001 gccacggtcg gccaccgcga ggataaccgt acgcttcaac gctttggaca gtatctgatg
     3061 acagtcgccc tcgggacttg acgtggtcga accgtcgtac agcgtgatct tgaccgcggg
     3121 aggtgccgcg cctttcttcg gctgctctgc gaagcgcgcc ttgcaaccga agagcgtcgg
     3181 ccacttcacc ggatttttgg ccgatgccgc cttgccctcg gccacgtcga ccagggcgta
     3241 gacccgatcg cctaagaggc catgctgggt gacctcggat tccgacagct cctcgccctg
     3301 catcgacttg accggatagc gccagaggga cgcgacttta ccgacctcgc tagccatgca
     3361 gacctctcaa ccgagacatt cgagtcgatc atagcatgga gtccagaagc ggaacacagg
     3421 cgaaccgggt ggctgggttg aagtctgaaa tcgcaactgg cgattgcaca ttacagagat
     3481 tcgatagcgg ccacttactc gggcacaatg ccgggcttcg ttggctcaca ttgcggttga
     3541 gagatggcct gcatctcggt gggctgctgc gtttccagct cgtccagcag aattgcaacc
     3601 aacatgtcgc tcatcacatt tacgccggag cgcacgcgcg ccaggatcca gtccacggtc
     3661 atgatgagcg gaatggcggc cgcgatgacg gcattcggca agcctgcggc ggagagcacg
     3721 agcggcagga caatcaggcc ggcttcagga atgcccgcta caccgatgcc ggccatgatc
     3781 gaggcgacga caatgaggag ctgatggccc atcgacagct cgtagccgag cgcttgcgcg
     3841 agaaagagcg cggccatcgc ttcatagagc gtgacgccgt cgttgttgag attcgtaccg
     3901 acgcaggcgg caagccgagc ggattgcggc gacacgttca tgcgctcgag acaacgcaac
     3961 gtcaccggca cggtcgcgag gctgctgttg cacgagacgg ccgtcatgat ggcgtctgcg
     4021 cccttgccga cgtaaatcct cggcgatttt tttcccacca gccaggccac gagcggatag
     4081 tagacgaacg cgtggaagag caggccaacc aggatggcgg cgagaaaaac ccagactgcg
     4141 gagaaaacgc cgagcccgac gcggccgacg atggaggcga ggacgccgaa caccgcaaac
     4201 ggcactaaga gaatgatcca ccagagcaca cgcacaagcc agcgatagat acgctcaatc
     4261 ccccgcacca gctgttccac gctgtgacgg ggcgcctcct ccgggccgcg aagacgccgc
     4321 agaagtgctc ccaacacgag cgccaggatc acgacgccga tgacgttgct gctcgtgaac
     4381 ggctggagca tcgtgcgggg aatgtaagag gcaatctcct ccaacggagt gccgctgccg
     4441 ccaccggcaa gcggggcacc ggcaggcgtc gagcctgagg cgaggttcag caattcgtcg
     4501 acacggctgg accaggaaag gcccggctgc cacgcattca tgattacaag gccgatcgtc
     4561 atcgcaacgg agacgttgat cagacagatg accagcagac gcgtgccctg gcgaagggga
     4621 aggatcgtac tgacgaaggc atcgagcacc gcaaagaaaa tgagcgggat ggcgagcgtc
     4681 ttcagcagca gaatgacgaa catgccggcg cggccgaggt gctcgttgcg cagcccgccc
     4741 agatacggct cctggccgag gaacgcgccc acggccgtgc cgcagatcac ggcgatcagg
     4801 acttgtgtat agagcgggag gccgaatgcg gaagaaggcg cgtggtcatc ggacgatgcg
     4861 gctggctgct gagactgatc gggaatggtc acttccgaag cacaaccaat ctcgtcgatc
     4921 cagtgtgaag tatggtcggg gcgaaaggat ttgaaccttc gacccctgcg tcccgaacgc
     4981 agtgcgctac cgggctgcgc tacgccccga ccgggtgagt ccttctgaaa ggagtcgcga
     5041 ttgtcttaca tcagaggggc gaaacgcaag ccatgcgcgt cggctacgcc tcgatgcgtc
     5101 acctgtccac gtgcgagatt cacacccttc gccaatccgg tatctgcacg cacggcggcc
     5161 tcgacgccaa aagaggataa ggtcaagaga tagggcagtg tcgcgttggt cagtgcctgc
     5221 gtggaagtgc gcgggacaat cccaggcata ttcgcgacgc aataatgcag cacgccatcc
     5281 acgacgtagg tcggatcgga gtgggttgtc ggcctgctcg tttctacgca accgccttga
     5341 tccaccgaca catccacgat gacggatcca ggcttcattc tggccaccaa cgcccgtggc
     5401 accagtttcg gcgctttggc gccgggaatc agcacggcgc cgatcaacag atccgccgtg
     5461 agcacgctct cctcgatcgc cgcccgagtg gaggcgcgag tgacgatgcg gccttgatag
     5521 agatcgtcca ggtcgcgcag ttgctcgatg tcgagattga tgacggtgac ttgtgcgccc
     5581 atcccgacgg cgattctcgt cgccgcgctg ccgacgacgc ctgcacccaa gatcacgact
     5641 ttcgcgggct cgactcccgg cacaccggcc aacaatagcc cgcgccctcc ttgagtgacc
     5701 tcaagataac gcgcgccgac ctgcaccgac atgcggcccg cgatctcgct catgggcttc
     5761 aacatcggga gtgtgccgtc cttggcttcg atggtttcgt aggcgatggc tgtgatgttg
     5821 gcttccagca gggccttggt gagttccgct gatgaggcta agtgcagata ggtgaacaac
     5881 acctgaccgg gccgaaagaa tccggtctca gagagctgcg gctccttgac cttcacgatg
     5941 agcgccgcct ctttgaacag gccttctttc gaggcagcga ggctggcgcc ggcctgccga
     6001 tactgctcat cggaatagcc gctgccctta cccgcggagg gctcgaccag gacctcatgt
     6061 cctgcttgcc tcagggcctg gacaccgtcc ggggtgacac tcacccgata ttcatgatct
     6121 ttgatttctt tcggcactcc gatgatcatg ggcgtccttt catttcatcg caacgcagct
     6181 gacgtctgtc tccattgtat cgggagatcg tccgaaagag aactgatgcg gccaaagctg
     6241 ctctcacgcg gtggacagtg cgggaaaggg tattgtaaga tttgcccttc gaatcgcaca
     6301 ttaagagtgc aggctgatcc tgacgagcca gaggaggcga tcttatggat gctgggggag
     6361 agtcatgcaa tgcctgcgga gcggccggga ccgcactcat gaaactgtcg ctcggaaaag
     6421 attttttcgg ccgcgcgtac gatcgcctct ccccttccac ggaccagagt ccgaagtggt
     6481 attgtgaaca gtgctccatg cagaagaatc tccaacgcga ctttcgtgac atccgcgccg
     6541 aattcgacaa gctgacggcc ggccacacct cggagctgtc aaagacggaa acgtttcaac
     6601 gggccgccat ccggttacgg gaaatcgcga ctattcttgg aggcagcacc tcaggatcgt
     6661 caccgtttct cgccgcgccg gacgtcgctc aactcatcgg ccgcttccaa agcacgacca
     6721 tgcaggccta ggaacaccgg aatacttgtc ctcgttcaag cgccatattt tcgtctgtat
     6781 caacgagcgc acgccggacg acccacgggg aagctgctcg aagttggggt cggaggccct
     6841 gcatgcgcgc ttcaagcaag aggccaagcg cctcaatctg aaggacatcg tccgggccaa
     6901 taaagccggc tgtctcgacc actgtgcttt ggggcccagt gtggtgattt atccggaagg
     6961 ggtctggtat accgtgagaa cggagtccga tgtgacggaa attatggaac gacacgtgat
     7021 caagggcgaa gtcgtgaccc gcttgctgat gccggaccat ccggccccga ccccactgct
     7081 accgttgagt tccttgcgct gaccatccat ggcgactgaa gataaatgga aggcgaacct
     7141 ggaaaaggtg gccttcatga aacaatttcc agggttactg ggggcgtggg aggccttgaa
     7201 gggcaagacc atcgaagcgg tgatcccgct caaaggcaag caaggcgctg ccgcgatcat
     7261 ctgtaacgat ggcacgttta ccgtcgcgcc accgatgatg accgagccct acgaactcgg
     7321 tgagaccttg acggcaggac gcacgctgct cgagcccaag caccgcgacg cgtatgtcga
     7381 atatgaccgg ctggcaaagc aggacaagga cgcgctgaag caggcgcgcc ttgagaaaat
     7441 catcggcgca atcgaaaaca atctggagca gattcccgaa ttaaaagcgc ggttgaaagc
     7501 cctcgtaagg gagtggccgt gagcagcatc cccaaaaaga acatgttcga gatcttctcg
     7561 caaggtctct ttgaaggagt caaacccatg atgatcgttc gcgaccacct ggtccgccac
     7621 cccgaccgct gcacccatca cgcggtctgc gtgcccgtgt gtccgaccgg ggcctggctc
     7681 tccacccccc cgtataagtt cgacgccgca cgctgcttgg aaagttgccg cctgtgcctc
     7741 gatgcctgcc cctctcacgc gatcttcgct gtcttcagaa aaggcgacaa gctgctcgag
     7801 ccgcagcaaa agcaataacc tcacgtcacg aaagttcggc ccggcggctt gatgccgccg
     7861 ggccgtgctg tttttttacg gaatgagcgc aaagtgttgc gtcgcgttcg ccgcagatag
     7921 atccacattg gcagattgtg cggcatagcc gtccgccgag gcacgaacgg cgtactggcc
     7981 tcccaccgcc ggcggttg
//
LOCUS       DASTQY010000460         4398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1069072, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000460 DASTQY010000000
VERSION     DASTQY010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069072"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..677)
                     /locus_tag="VFR82_14810"
     CDS             complement(117..677)
                     /locus_tag="VFR82_14810"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4502919.1"
                     /translation="MKNAAGFILIGTLGLGSMLMQACASQSGSVGGDEFAQEQITEPM
                     IKHIPPPEYSMNTSRRPSQRAELSAVNATGLPFGALSDVLFDFDQAVLRRDALPLVEA
                     NARRLVQDGVTRLLLEGRGDEVGTSAYNIVLGERRARSVKSYLQERGLSLQLKTTSYG
                     KDRPLCYQHNEECMQKNRSVHFLVKD"
     gene            complement(921..1169)
                     /locus_tag="VFR82_14815"
     CDS             complement(921..1169)
                     /locus_tag="VFR82_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502920.1"
                     /translation="MEICTTLGEVNMNNLRKEVRELISINEKIQSALLHGDRMTDDEA
                     TLIRQCASELLEKIPPPSRTWTRAGRTEEGGEAVAGNP"
     gene            complement(1247..1636)
                     /locus_tag="VFR82_14820"
     CDS             complement(1247..1636)
                     /locus_tag="VFR82_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502921.1"
                     /translation="MPNTNASFSPRMRSDELKEQIGKLERLEHRTPVLPTLDGFDAET
                     EELLGRLYGDSHKYLEAYKYATVAESEALVNLPESAQEPMAKDLPKTAVQQRRQVLLG
                     ILTEMQDMEAKEVAMLTGEDREDPPSI"
     gene            complement(2007..2189)
                     /locus_tag="VFR82_14825"
     CDS             complement(2007..2189)
                     /locus_tag="VFR82_14825"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEU4502922.1"
                     /translation="MARYGKAAQKSVKRTMHKRKKGTLTSGKSGKRVKNRKQAIAIGL
                     SEARRKGAKVPRKKAA"
     gene            complement(2277..2588)
                     /locus_tag="VFR82_14830"
     CDS             complement(2277..2588)
                     /locus_tag="VFR82_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502923.1"
                     /translation="MKSIMLSFLAAGLFCFGISVAQAANPMSEKEASPTIKERITKDT
                     VKGTLMKTEGEYYWIKDTDGKEVKIHVDKSTKMDKVVVGDMVKAYITDKGHTTTLQRA
                     D"
     gene            complement(2726..3019)
                     /locus_tag="VFR82_14835"
     CDS             complement(2726..3019)
                     /locus_tag="VFR82_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502924.1"
                     /translation="MIARPQHYDRKEVVVMGQVSGVQAITDKQGQPAFQFLLKDNVGT
                     IKVISRREVQAGDQIIVEGTFTRRRQGGRLAVYNEVNAIVIRPLNQFDADLVG"
     gene            3423..>4398
                     /locus_tag="VFR82_14840"
     CDS             3423..>4398
                     /locus_tag="VFR82_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEU4502925.1"
                     /translation="MSDSAIVTFALIAACAGIGYGLYLAMWVFRLDAGNAKMQEIAKA
                     IQEGAGAYMNRQYKTVGVVAAVLFVVLAAAGAVSDKFGILTAVGFLVGAGASAIAGYV
                     GMIIAVRANVRTAQAAHQGMNAALTVAFRGGAVTGLLLIGLGLLAITGFFMIAQSISG
                     QEKAVHALLSLGFGGSLISVFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVI
                     ADNVGDNVGDCAGMAADLFETYAVTTVAAMVLAYTMFKGASAPILYPLALGGVTIFAT
                     IIGIFFVKVGPGGEIMPALYKGLVVAGGIAAIAFYPVTTQVMEGIGGVS"
BASE COUNT          830 a         1214 c         1276 g         1078 t
ORIGIN      
        1 gtgaacgtga ttagggatag cgaccggaac gttttcccaa gcaaaagata aggcccgctt
       61 cctttaagaa gcgggcctta cacctgtctt cacctattgc ggtgagaaat gccggattag
      121 tctttcacta aaaagtggac actgcgattc ttctgcatgc attcttcatt gtgttgataa
      181 cacagcggac gatctttgcc atagcttgtc gtcttcagct gaagcgaaag tcctcgctcc
      241 tgcaggtaag acttcacact tctcgcccgg cgctcgccca gcacgatgtt gtaggccgac
      301 gtgccgactt cgtcgccgcg accctcgagc aataagcgcg tgactccgtc ctgcacgagg
      361 cgtcgcgcgt ttgcctctac caagggaagg gcatcgcgcc gcaacacggc ctgatcaaaa
      421 tcgaagagca cgtcagacaa ggcgccgaac ggcagacccg tcgcatttac cgctgacagc
      481 tccgctcgtt gggacggccg ccgagaggtg ttcatgctgt attccggagg aggaatgtgt
      541 tttatcatgg gctcggtaat ctgttcctgg gcaaattcat cgcccccgac tgaacctgat
      601 tggctagcac aggcctgcat cagcatcgac ccaaggccaa gcgttccaat gaggatgaat
      661 ccagctgcgt tcttcatgct gcccctcctt gtgcggatga accgtttcac aggtaagcaa
      721 ggggggtgcc attggatcgt cgacagtcgc ggactcggaa aataggactc ccggtgccgc
      781 ggtgcctcat agattaagag gaaatctgat gttagggcag gtggatgggc cagtgcagtc
      841 tcagagccgt accttgtctg aagcctgtcg catgcgaggt ttgtacagaa gttgcctttt
      901 ttgtcgctcg actgagcggc ttacggatta cctgcgaccg cttcaccgcc ctcctccgtt
      961 cggccggctc ttgtccaggt gcgggatgga ggcgggatct tttccagcag ctcgcttgca
     1021 cactggcgga tcagggtcgc ctcatcgtcg gtcatgcgat caccgtgcaa cagagcgctc
     1081 tggatttttt cgtttatgct aatgagctca cgaacttctt tgcgcaggtt gttcatgttg
     1141 acctccccga gcgtggtaca aatctccatg aaatctcctt gtcgcaatgg agatcacata
     1201 atcatgcgct cgacctggtc aagggggata gaagaaggcg gtgcagctag atactcggag
     1261 ggtcttctcg gtcctctccc gtcagcatcg ctacttcttt ggcttccata tcctgcattt
     1321 cggtgaggat gccaagcaac acctggcggc gctgttgtac ggcggttttg ggtaaatcct
     1381 tggccatcgg ttcttgtgca gattcgggaa ggttgacgag tgcttcgctc tccgcaacgg
     1441 tggcatactt ataggcttcg aggtacttgt gactatcccc ataaagccgg cccaagagct
     1501 cttcggtctc tgcatcgaac ccatcgaggg tgggcaggac aggcgtacgg tgttccaacc
     1561 gttcaagttt tccaatctgc tccttgagtt catcgctccg catgcgcgga gaaaacgagg
     1621 cgttggtatt gggcattgag gtgctccttc cttcttcatc ttctgctgat cagcttgtac
     1681 aattcggttc gcttcttgaa ctgggaagct cccctgacta gcattgcctg cgaaacttgg
     1741 cgaactggcg ttgcaagtcg ttcaagtcgg tttcagacat ctcccctaac tcgatcaatt
     1801 tatagagcgc tcttcaagcc gccaactgcc tggtaaactt gtgacagata tcggtcacat
     1861 tgttgatgtg gtgcgcccgt tgttctgtcc tgccatgttt ctttgatcgc gagagatgcc
     1921 gctggtgtgg gagggtggta ggaaatgtgg ccgtcctgga aaggcaggac ggccgtgcaa
     1981 accaggcagc tgttggacag tgagcgttac gccgccttct ttcgtggaac cttggcgcct
     2041 ttccgtcggg cctccgacaa tccgatggca attgcctgct tgcggttttt tacccgcttc
     2101 ccgcttttgc cgctggtcag tgtccctttt ttccgcttat gcatcgtccg cttcaccgat
     2161 ttctgagcag ccttcccgta gcgagccatg cacacacccc ccttcgcggc gaaaaaatca
     2221 tgtgcggatg ggcagacgcc ggcaggttgc cggcgtcata gcttggtcgc gaacagctaa
     2281 tcagcgcgtt gcagtgtggt ggtgtgcccc ttgtcagtga tgtaggcctt gaccatatct
     2341 cccacgacga ccttgtccat cttcgtgctc ttgtcgacgt gaatcttcac ttctttcccg
     2401 tcggtatcct taatccagta atactcgcct tccgtcttca tcaaggtgcc cttgaccgtg
     2461 tccttggtaa tgcgctcttt gatcgtgggc gaggcttctt tctctgacat cggattggcc
     2521 gcttgcgcga cgcttatgcc gaagcagaaa agcccggccg cgagaaatga cagcataatc
     2581 gacttcatgg aatcctcctt gtggtgtggg tgtcacacgt acgccctttg gggtggcgca
     2641 cgacatctcc ctctacacac tgtcgtacgg gactccctga gtgtgtacta gtaaaccgcc
     2701 tagaaaatgt ggggagggcg cgggactatc ccacgaggtc tgcgtcaaac tgattgagag
     2761 ggcggatcac gattgcgttc acttcattgt agaccgcgag tcgtccgcct tggcggcgcc
     2821 gggtgaaggt accttcgacg atgatttggt caccggcttg aacttctctg cggctgatca
     2881 cttttatggt acccacgtta tctttcaaca agaactggaa ggctggctgg ccttgcttgt
     2941 cggtgatcgc ctggaccccg ctgacctgac ccatgaccac cacttctttt cgatcgtaat
     3001 gctggggtcg tgctatcacg tcagctactt cgagcaactc cgctgcagcg ggtgccgcag
     3061 cgagcaggga gactaatccg aaaaccatcc aaattgaggg gtgaatcaag ggtctgactg
     3121 aggtgtgaac tagggttttg cctgtcatct gcgggggtat tatagcgatt ttgtttcctt
     3181 tgacaagctg ggcgttcacc ctagaggtct cgcccgatca tttgccccct gccgtatgcc
     3241 ttttacacct tgatcccccc acgccatctg tatagaatgg cgcgtttttt ccatgcagct
     3301 gcctcagccg ttccgcacca ccttcggttc tccctagatc tacatgaagt gtcctatggt
     3361 gagcgcaaag ggacgggaat ggttgtgaca acagacttcg taacctggga ggagggcatc
     3421 cagtgagtga ttcggccatt gtgacgtttg cattgattgc cgcctgtgct ggcatcggct
     3481 acggtcttta tctcgcgatg tgggtgttca gattggacgc cgggaacgcc aaaatgcagg
     3541 aaattgccaa ggccattcaa gaaggcgccg gtgcgtacat gaatcggcag tacaagaccg
     3601 tcggcgttgt agcggcagtg ctcttcgtgg tcctggccgc ggccggcgcc gtctcggaca
     3661 agttcgggat actgacggcg gtagggtttc ttgtgggagc cggagcctcg gcgattgccg
     3721 gctacgtcgg aatgatcatc gcagtgcgtg caaacgtgcg tacggcgcag gcggcgcatc
     3781 aggggatgaa tgccgccttg acggtagcct ttcgcggcgg cgctgtcacg gggttgttgt
     3841 tgatcgggct ggggctcctc gccatcacgg gatttttcat gatcgcgcaa tcgatctcgg
     3901 gacaggagaa agccgtacac gccttgctga gtctgggatt cggcggcagc ctgatttcgg
     3961 tgttcgcgcg tgtcggcggc ggcatctaca ccaaggcggc cgatgtcggg gcggacctgg
     4021 tcggcaaggt cgaggcggga attcccgagg acgacccacg caacccggcc gtcattgcgg
     4081 acaacgtcgg cgacaacgtc ggcgattgcg ccggcatggc ggcagacctc ttcgaaacct
     4141 atgccgtgac caccgtcgcc gcgatggtgt tggcatacac gatgttcaaa ggcgccagcg
     4201 caccaatcct ctatccattg gcgttgggcg gcgtaacgat ctttgcgacg atcatcggca
     4261 ttttcttcgt gaaggtgggg ccaggcgggg aaatcatgcc ggcgctgtat aaaggcctgg
     4321 tcgtcgccgg cgggatcgcg gcgatcgcgt tttacccggt cacgacgcaa gtcatggaag
     4381 gcatcggcgg cgtgagtg
//
LOCUS       DASTQY010000461         3861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_511348, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000461 DASTQY010000000
VERSION     DASTQY010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511348"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFR82_14845"
     CDS             complement(<1..207)
                     /locus_tag="VFR82_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502926.1"
                     /translation="MGPSRGRRYFLLLLMLAGFAVILFRLVSLQVLQAAELTARADRQ
                     HQKSVTLEGARGTVTDRHGKVLAMN"
     gene            complement(210..533)
                     /locus_tag="VFR82_14850"
     CDS             complement(210..533)
                     /locus_tag="VFR82_14850"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU4502927.1"
                     /translation="MKSIAIIAGVCLVFFFVWERVDIVRLGYHIERLKAQKVLLERER
                     DQLQVKFSALTAPERIAKVATDKLGMVPPQQGQVLIVHPQQAPPPPLDQVRIARTIST
                     AKGER"
     gene            complement(530..1486)
                     /gene="rsmH"
                     /locus_tag="VFR82_14855"
     CDS             complement(530..1486)
                     /gene="rsmH"
                     /locus_tag="VFR82_14855"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU4502928.1"
                     /translation="MAWENVGEIGGSQYRGNREHLPVMPAEVLFWLKPRTQGIYLDCT
                     VGYCGHALDILQASDPDGKVFGIDRDPEAVDAARQRLQPFGDRAVIIKGHFVELKRLL
                     AERSISQVEGVLFDLGASSPQLDDPARGFSFQSEGPLDMRMDRSGPTAADIVNGLDEI
                     ELADMIYRWGEERYSRRIARALVRARQQRTISTTRQLALLIESAVPGHYRRGRIHCAT
                     RTFQALRIAVNKELDHLEASFRDAADVLAPGGRLCVISFHSLEDRIVKQSFKAFSNRH
                     TGEFEVLTKRPQVPTEDEIRGNPRSRSAKLRVLQRIVNEGHA"
     gene            complement(1721..3013)
                     /gene="asnS"
                     /locus_tag="VFR82_14860"
     CDS             complement(1721..3013)
                     /gene="asnS"
                     /locus_tag="VFR82_14860"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU4502929.1"
                     /translation="MPVMTIDAAAQHVGQEVTLRGWLRSRRDSGKLHFLTLRDGTGDM
                     QAVVSKKAVGDAQFALSASLTQESSLVVTGQLRQDNRAPGGYELDVRSIEALQIAGPF
                     PIQPKEHGVGFLMEHRHLWLRSHRQHAILRIRHEIIRACRNFFDERGFVLVDSPIFTP
                     NACEGTTTLFQTQYFDETAYLTQSGQLYSEATAAAFGKVYCFGPTFRAEKSKTRRHLM
                     EFWMVEPEVAFAQLDDMMDLAEQFLTAIVQQVLETRRTELTTLERDISKLERVAPPFP
                     RLTYEEAVGILRRKNNPTQMGDDFGGDEETILSQEFDRPLIIHRYPAALKAFYMQPDD
                     QRPDLALCMDVLAPEGYGEIIGGGQRIHTLELLRTRLQEHRLPEEAFKWYLDLREFGS
                     VPHAGFGMGIERAVAWICGLEHVRETIPFPRMLYKLYP"
     gene            3140..>3861
                     /locus_tag="VFR82_14865"
     CDS             3140..>3861
                     /locus_tag="VFR82_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502930.1"
                     /translation="MYTGWHLRSHFVMALLVGSGAYGGLSFSSASAASAIYYCADRRG
                     DQLLSATPGPGCVPLAEAKQERRDESVQDKRRREFHIENLKQDVSAFLTRYRHFLECC
                     KNDLSELNDIEELGDEVNELLISAQANLSDNALASRAIMLRELIPRVAKARSDLKTLR
                     ATLGKIRELSATRDQADFEEAGRQSQRIRELEEFIERDIHAPKLPGSAKTGASIGVAP
                     AAGPAIGSSSKTGTDIGGTGSTG"
BASE COUNT          880 a         1100 c         1049 g          832 t
ORIGIN      
        1 gttcatcgcc aacactttgc cgtgccggtc ggtcaccgtt cctctcgcac cttccaatgt
       61 gacgcttttt tgatgctgcc gatcggccct cgctgtcagt tcagccgcct gcaacacctg
      121 taggctgaca agccggaata agatgacggc gaacccggcc agcatcagga gtaacaagaa
      181 ataccgccgg ccccgggatg gacccatact tatcgttctc cttttgccgt cgagatcgtg
      241 cgagcgatgc gcacctggtc aaggggcggc ggcggagcct gctgtggatg gacaatcaag
      301 acctgtcctt gctgcggcgg caccattccc agcttatctg tggcaacttt ggcaatgcgt
      361 tccggcgccg tcagggcgga aaacttcact tgcagctggt cccgttcgcg ctctagcaac
      421 accttctgcg ctttcaagcg ctcgatgtga tatcccaatc gaacgatatc cacgcgctcc
      481 cagacaaaga aaaacacgag gcagacgccg gcgattatgg cgatggattt catgcgtgcc
      541 cctcatttac gattcgctgc agaactcgaa gcttggcgct ccgggatcgg ggattgccgc
      601 gaatctcatc ttcggtaggc acttgcggcc gcttcgtcaa gacttcgaac tcgccggtgt
      661 gccgattgga aaaggccttg aaggactgtt tcacaatgcg gtcttcgaga gaatgaaacg
      721 agatgacaca gagccgtccc ccaggcgcca agacatccgc tgcatcacgg aacgacgcct
      781 cgaggtgatc caactcttta ttgacggcga tgcgaagcgc ctggaaggta cgcgtcgcgc
      841 aatgaatgcg tcctcggcgg taatgtcccg gcactgccga ctcaattaac aatgccagtt
      901 gccgagtcgt cgaaatcgtc cgttgctgcc gagcgcgtac gagtgctcga gcaatccgtc
      961 gcgaatagcg ctcttcaccc cagcggtaaa tcatgtccgc caactcgatc tcatcgagtc
     1021 catttacgat atcagccgcc gtcggaccac tgcgatccat gcgcatatcc aacggcccct
     1081 ctgattgaaa gctaaaacca cgagcgggat cgtcgagctg tggagacgac gcaccaagat
     1141 cgaacaggac cccctcgact tgtgaaatgc tgcgctccgc gagaagccgc ttcagttcca
     1201 caaaatgtcc cttgataatg actgcccggt ctccgaacgg ctgcagtcgt tgccgagccg
     1261 catcaacagc ctccgggtca cgatcaatgc cgaagacttt cccgtccgga tcactggctt
     1321 gaagtatgtc aagcgcatgc ccacaataac ccacagtgca gtccaggtag attccctgag
     1381 tacggggctt aagccaaaat aagacttctg cgggcataac cggcaagtgc tctcggttgc
     1441 cacgatactg acttccacca atttcaccca cattctccca cgccatggcg gagtatactc
     1501 ttgactctta cattgtcaac aataatttca tggacttaca aatatttttt ctaacgcctg
     1561 tgggtagaca aggtatgccg gttgaccccc cgggaaagag gcagatatcc acagttaaag
     1621 tgggggattt tatggtctgg ctggttttac cactactagg cgttgtgctg ggtttgcaag
     1681 gatgcgagcg agcaggtaga agttccgagg aagcgagaaa tcaggggtaa agcttataga
     1741 gcatgcgagg gaaggggatg gtctcgcgaa cgtgttccag gccacagatc caagctaccg
     1801 cgcgttcaat gcccatcccg aagcccgcat gcgggacgga accgaactcg cgcagatcca
     1861 ggtaccattt gaacgcttct tccggaagcc gatgctcttg taaacgcgtc cgtagcaatt
     1921 ccagcgtgtg gatccgttgc ccgccgccga taatctcgcc gtatccttcg ggcgccaaca
     1981 cgtccatgca gagagcaaga tcgggccgtt gatcatcagg ttgcatgtaa aaggctttga
     2041 gcgcggccgg atagcgatgg atgatcaacg gtcgatcgaa ttcctgtgac agtatggttt
     2101 cttcgtcgcc cccgaaatca tcacccatct gcgtgggatt attcttcctg cgtagaatac
     2161 cgactgcctc ttcatacgtg agacggggaa atggcggcgc tacgcgctca agcttactaa
     2221 tatcccgctc taaggtcgtg agttctgttc ttctggtttc caatacttgc tgcacaatgg
     2281 cggtaaggaa ttgttcggcc aggtccatca tgtcgtccaa ttgtgcaaag gcgacttcag
     2341 gctccaccat ccaaaattcc atcaaatgtc gccgcgtctt cgatttttct gcgcggaacg
     2401 tcggcccgaa acaatacacc ttaccgaagg ctgcggccgt ggcctcacta taaagctgcc
     2461 cgctctgcgt caaataggcc gtttcgtcaa agtactgcgt ctgaaagagc gtggttgtgc
     2521 cctcgcaggc attcggcgtg aaaattggtg aatccaccaa gacgaaaccg cgttcgtcga
     2581 aaaaatttcg acacgcgcga atgatctcgt gacgaatccg cagaatcgca tgttgtcgat
     2641 gtgaacgcag ccagagatgc cggtgttcca tcagaaatcc gacgccatgc tccttcggtt
     2701 ggattggaaa cggtccggca atttggaggg cttctatgct gcgtacgtca agttcatacc
     2761 ctcccggtgc gcgattgtct tgccgaagct gtccagtcac taccagtgag gattcttgtg
     2821 tcaaactcgc cgacagtgcg aattgagcat caccgacggc tttcttgctg acaactgcct
     2881 gcatgtctcc ggttccgtcc cgaagagtaa gaaaatgaag cttgccgctg tcacggcggc
     2941 tgcgcaacca gccgcggagg gtaacttcct gtcctacatg ctgtgccgcg gcatcaatcg
     3001 tcatcacggg catcgcctgt cctcctaaaa aacgttgagc ttggactcgg gcagactagc
     3061 cgattgtccg ggctcgttca agaagagcaa cattgctcgt ttcattgaca agatgtggcc
     3121 gtgagataag aatggaagca tgtatacagg ctggcatctg cgcagtcact ttgtgatggc
     3181 cctcttggtg ggaagcggcg cttacggtgg tctttctttc tccagcgctt cagcggcctc
     3241 ggccatttac tattgcgcag atcgacgggg agatcagctt ctctcggcga cacccggacc
     3301 aggctgtgtt ccgctggcag aagccaaaca ggaacgaaga gatgaatctg tccaggacaa
     3361 gcgccgacgg gaatttcata tagagaacct caagcaggac gtttctgcgt ttctgacgcg
     3421 ataccggcac tttctggaat gctgcaagaa cgacctcagc gaattgaacg acatcgagga
     3481 gctcggcgac gaagtgaacg aattactgat ctccgctcag gccaatctct ccgacaatgc
     3541 actcgcatca agggcgatca tgctgcggga gctgattccg cgagtcgcca aggccaggag
     3601 cgacctcaag acgctgcgtg caactctggg aaagatccgc gaactctccg cgacacggga
     3661 tcaggctgac tttgaagaag cgggacggca atcgcaacga atccgggaac tcgaggagtt
     3721 tatcgaacgg gacatccatg ctccgaagct gcctgggagc gccaaaacgg gagccagcat
     3781 cggtgtcgcc ccagccgcgg gccctgcgat cggcagcagc tcaaaaaccg gcacggatat
     3841 tggggggaca ggatcgaccg g
//
LOCUS       DASTQY010000462         4202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_512034, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000462 DASTQY010000000
VERSION     DASTQY010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512034"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VFR82_14870"
     CDS             complement(<1..406)
                     /locus_tag="VFR82_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEU4502931.1"
                     /translation="MHPEKPNRAIQSAHGHLVQLASDVLAMAKKDGATEADVVVADGE
                     NLSVQVRLGSVDRLTKAREKRLGLRVFVGKRSATTSTSDFSSESLRRLVSETCALARA
                     VVEDSMSGLPEPEQLAQEQPDLDIYDDTKVDAE"
     gene            complement(423..1856)
                     /gene="tldD"
                     /locus_tag="VFR82_14875"
     CDS             complement(423..1856)
                     /gene="tldD"
                     /locus_tag="VFR82_14875"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEU4502932.1"
                     /translation="MAQLIPLDSFGVTERDVEGALGRVKVRNVDYADLYFESCVSESV
                     SMEESLVKRATKSISQGVGVRATAGEKTGFAYSDELTKRDLELAADTARYIAHSPGGT
                     EPVPVPVRSRPTRDLYPVSHEQLEVATAERVALLNRIDEEARRYDPRIKNVMASFNTE
                     YKVVMVATSEGTLIGDVQPLSRLQVTCIAEEHGNRQIGSFGGGGRIGFEFYRERDRYL
                     DYAKEAARIAILNLSSVDAPAGVMPVVLGGGWPGILLHEAIGHGLEADFNRKKTSAFS
                     NLIGKRVASEVCTIVDDGTLPGRRGSLNMDDEGTPTSRTTLIEKGILRGYITDRLNAH
                     LMGIPVTGNGRRESYQSIVLPRMTNTFMLAGQSDPNEIIRSVDKGLYAVSFGGGQVDI
                     TNGKFVFSASEAYLIEGGKITRPVKGATLIGSGPDILTKVSMVGHDLKLDEGIGTCGK
                     EGQSVPVGVGLPTIRIDQITVGGTQAA"
     gene            2076..2861
                     /locus_tag="VFR82_14880"
     CDS             2076..2861
                     /locus_tag="VFR82_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU4502933.1"
                     /translation="MDTQPPSRNDDLSETELTAKLDPDLLPKHVAIIMDGNGRWAEAR
                     SLPRIAGHREGIKSVRDMISLCLELGIQALTIYAFSQENWNRPTTEIDGLMGLLEHYL
                     STERNKLIEQGVRFRTIGRTEALPSSALQWVRAAEQETARMEKLQLTVALSYGGRAEI
                     VDAVRGLVRDAQEGKLQPEDIDEPQIQQYLYTRGLPDPDLLIRTSGETRISNFLLWQL
                     AYTELYFTPTLWPDFRRRELLLALLEYQRRERRFGRVFSSLSS"
     gene            2882..3757
                     /locus_tag="VFR82_14885"
     CDS             2882..3757
                     /locus_tag="VFR82_14885"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU4502934.1"
                     /translation="MLECGTTNRDPAQTVRKAAARFDARRIYTVLFVVPLLYAAIRFF
                     PPVVFAGVVAVAGCLSLHELYRLCFQVGRQPFMVGIGLAGCVALIVSPHVPGMAQPGL
                     LVPLVAILSIPLLIRTPLDQSVKDAALALTGMMYIGLTLSYLVAVRLLPQGEWLLFFL
                     LLVTWAADTGAYYVGTLYGQRPLAPRISPKKTIEGLAGGLFAALLIAFVARWGFLSDI
                     SYWDGLVLAVLLTLAGLWGDLVESAIKRSVGVKDSGGLLPGHGGMLDRLDSLLFTAPT
                     FYYYVTSIRGIGPST"
     gene            3899..>4202
                     /locus_tag="VFR82_14890"
     CDS             3899..>4202
                     /locus_tag="VFR82_14890"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248075.1"
                     /note="catalyzes the NADP-dependent rearrangement and
                     reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to
                     2-C-methyl-D-erythritol 4-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEU4502935.1"
                     /translation="MKSIVILGSTGSIGTNTLDIVERFPEEFRVVGLTAGNNDEMLAE
                     QIRRFHPPVVAMPTESAAARLKQRCGGLPVEILSGPEGLAAIASLPEAELVVSAIVG"
BASE COUNT          836 a         1264 c         1199 g          903 t
ORIGIN      
        1 gttcggcgtc caccttcgtg tcatcataga tatccaggtc cggttgttcc tgcgccagtt
       61 gctcgggctc aggcagcccc gacatggaat cctcgacaac agccctggcg agcgcacagg
      121 tctccgacac cagacgtcgc aacgactcgg aggagaaatc ggatgtggag gtagtggcgg
      181 aacgcttgcc gacgaagacg cgaagcccca gccgtttttc gcgcgccttg gtcagccgat
      241 cgacagatcc gagccggacc tgcaccgaca ggttttcccc gtcggcaacg acaacatcag
      301 cttcggtcgc gccgtctttt ttggccattg ccaatacatc agaggcaagc tggaccagat
      361 ggccgtgggc agattggatc gcgcgattag gtttctctgg gtgcatcaca catcaccttg
      421 cttcatgctg cttgggttcc tccaaccgtg atctgatcga tccgaatcgt cggcaatccg
      481 acgccgaccg gcaccgattg cccctccttg ccgcaggtgc cgatgccttc atcaagtttc
      541 aaatcgtggc cgaccatcga gaccttcgtc agaatgtcgg gcccgctgcc aatgagtgtc
      601 gcgcccttga cgggtctggt gatcttcccg ccttcgatca aatatgcttc actggcggaa
      661 aacacgaact tcccattggt gatatcgacc tgccctcccc cgaatgagac ggcatacaac
      721 cctttgtcga ccgaacggat aatctcgttc ggatccgact gtcccgccag catgaaggta
      781 ttggtcatac gcggcaggac gatgctttgg taactttcac gccgcccgtt tccggtcacc
      841 ggaattccca tcaaatgtgc gttcaacctg tctgtgatgt aaccccgcaa gatgcccttc
      901 tcgatgaggg tcgtccgact ggtcggcgtg ccttcgtcat ccatattcaa cgagccacgc
      961 cggccgggca gcgtcccgtc atccacgata gtgcaaacct cggaggccac ccgtttgccg
     1021 atcaaattgg aaaacgccga tgtcttcttg cggttgaaat ccgcctccag gccatggcca
     1081 atggcttcgt gcagaagaat cccgggccag ccaccgccca gcacgaccgg catgactccc
     1141 gccggagcgt cgaccgaaga aagattgagg atcgcgatgc gggcagcctc tttcgcatag
     1201 tccaagtaac ggtcccgctc gcgataaaac tcaaacccga tacgaccgcc gcccccgaaa
     1261 gagccgattt ggcggttgcc gtgttcttca gcgatgcagg tcacctgcaa tcgggacaag
     1321 ggctgtacgt cgccgatcaa cgtcccttca gatgtggcca ccatcacgac cttatactcg
     1381 gtgttgaagg aggccatcac gttcttgatg cgcggatcgt accggcgcgc ttcctcgtca
     1441 atgcggttga ggagcgcaac ccgctcggct gtcgccactt ccagctgctc gtgggaaacc
     1501 ggatacagat cacgcgtcgg tctggaacgc accggcacgg gcaccggttc ggtcccgccg
     1561 ggagagtggg cgatatatcg ggcggtatct gcagctagct cgagatcccg cttcgtcagt
     1621 tcgtcggaat aggcaaaacc ggttttttct ccggctgtcg cgcggacccc gaccccttgg
     1681 gaaatgctct tcgtcgcccg tttcacgagc gactcttcca tcgatacgga ttccgaaacg
     1741 caagactcga aatagaggtc cgcataatcc acgttgcgaa ccttgacccg gcccaaggcc
     1801 ccctcgacat cgcgctcggt gacgccgaat gaatccaacg gaataagttg tgccatcgcg
     1861 caagaatcct tagaagaggg ttttaaaatt gactcatgga tgcgagaagc caaagtatag
     1921 ccgattcaat tttccaggcg caatcagagc gcgtcgaact cttcgctttt ccaaggattt
     1981 cgggaaatat cgtttgactt tcacagagac cgtcagtaga ctttcgtcac ctgccatacg
     2041 cacgccatag aaaggaccat aatccatcag agctcatgga cactcagcca ccttccagaa
     2101 atgacgacct gtcggaaaca gaactgacgg caaaactcga tcctgacctt cttcccaaac
     2161 acgtcgccat catcatggac ggcaacgggc gctgggccga ggctcgaagt ttgcccagga
     2221 ttgccggcca ccgcgagggc atcaagtcgg tacgcgacat gatctcgtta tgcctggagt
     2281 tgggcattca ggcgctgact atttatgcgt tttcgcagga aaactggaac aggcctacga
     2341 cggaaattga cggcctcatg gggctgctgg agcactacct ttccacagag cgaaataagt
     2401 tgatcgaaca gggagttcgt tttcgcacga tcgggagaac cgaggccctg ccgagttctg
     2461 ccctgcagtg ggtgcgcgcg gccgaacagg aaacggcgcg catggagaag cttcaactga
     2521 ctgtcgcact cagctacggg gggcgcgctg agattgtcga tgccgtccga ggactggttc
     2581 gggacgccca ggagggcaag ctgcagcctg aggatatcga tgaaccgcag attcaacagt
     2641 acctgtatac gcgcggtctg cccgatccgg acctgttgat ccgcaccagc ggggaaactc
     2701 gcatcagcaa ctttctcctg tggcagttgg cttacactga attgtatttc acaccaaccc
     2761 tctggccgga cttccgccgc cgggagctat tactggctct gctcgagtat caacggcgcg
     2821 aacggcgttt cggccgtgtc ttcagcagcc tgtcgtcgta gcctgccgga gaatcatcga
     2881 catgttggag tgtggaacga cgaatcgaga cccggcgcag accgtccgca aggccgcggc
     2941 tcgattcgac gcccgtcgga tctacacggt tctgttcgtc gttcccctct tgtatgcggc
     3001 aatccgcttt tttcctcccg ttgtgtttgc cggagttgtg gcggtggccg gctgtctctc
     3061 tctccacgag ctgtatcgct tgtgtttcca ggttggacgt caaccattca tggttggaat
     3121 cggcctggca gggtgcgtcg cgttgatcgt cagtcctcac gtgcctggta tggcacaacc
     3181 gggtctgctc gtaccccttg tagccatcct ctccatcccc ctgctgatca gaaccccgtt
     3241 ggatcaaagc gtaaaggatg ctgccctcgc cttgaccggc atgatgtaca tcggcctgac
     3301 gctgagctac ctggtcgcgg tccggcttct tccgcaaggc gagtggctgc tcttctttct
     3361 gctgttggtc acttgggcag ccgacacagg tgcttactac gtcggcacgc tgtacggtca
     3421 gcggccactg gccccgagga tcagtccgaa gaaaacgatc gaaggtctgg caggcggcct
     3481 gtttgctgca ttgctcattg cctttgtcgc acgatggggg ttcttgtcgg acatatccta
     3541 ttgggatggt ctggtgctcg ccgtgctcct gacgctcgcc ggcttgtggg gagacttggt
     3601 ggaatcggcc atcaaacgca gtgtcggcgt gaaggattcc ggcgggctgc tgccgggtca
     3661 tggcggtatg ctcgatcggc tcgatagtct cttgttcacc gcgcccacgt tctactacta
     3721 tgtcacttcg attcgcggga ttgggccgtc gacgtagaac caggtgttcg gaggaactgt
     3781 tgcaggatgt tcaaaatgac cgctgttctc acccgcccga ccctgggcgc gccgagacgc
     3841 gccatttccc aagtgaggcg caacgagtga agaggcgaga aggagccaag gagccgttat
     3901 gaaatcgatc gtgatcctgg gctcgaccgg atccatcggc accaatacgt tggacatcgt
     3961 ggagaggttc cctgaagaat tccgcgtcgt ggggctcacg gccgggaaca atgacgaaat
     4021 gttggccgaa cagatccgcc gttttcaccc accggtcgtc gccatgccga ccgaatcagc
     4081 cgcggcccgg cttaagcaac ggtgcggggg tctgcccgtc gagatcctga gcggaccgga
     4141 aggcttggcc gccatagcct cgcttccaga agccgagctg gtggtctcgg ccattgtcgg
     4201 ag
//
LOCUS       DASTQY010000463         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1721787, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000463 DASTQY010000000
VERSION     DASTQY010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721787"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VFR82_14895"
     CDS             complement(<1..557)
                     /locus_tag="VFR82_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502936.1"
                     /translation="MKSEKRWMSYWRKSAVRLVPYLTVLCLVAAGCTTPVHREEADMK
                     PATVDELTALLQQRELAVRTMKGLFSAKVRGGVIPITTRVEGAVYYRRPSAMRLRGFT
                     AIGSELFEFLQTDDQFTLRLPTMGRVLSGHPSDMSEMGKLARPFQLSVWAMSGVLGTG
                     TINGSETARVVDEGDRYRLEVFRASS"
     gene            complement(520..1881)
                     /gene="radA"
                     /locus_tag="VFR82_14900"
     CDS             complement(520..1881)
                     /gene="radA"
                     /locus_tag="VFR82_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU4502937.1"
                     /translation="MKSKSIFSCQACGHQSPRWLGRCPDCGGWNTIKEERHAPTGKGR
                     PVAMNVAQPAATPIAKIEVIGEDRRLTRISEFDRVLGGGVIPGAVILIGGDPGIGKTT
                     LLLQALPRLASNVQPVLYVSGEESPRQIKMRGQRLGVEHPNLLVLAETSLEQILKAVQ
                     EIQPAAVVVDSIQTVYTEQLTSAPGSISQVQEVSGQLMWFAKRSGVPVFIIGHVTKEG
                     AIAGPRLLEHIVDTVLYFEGDKGHSYRILRAVKNRFGSTNEIGVFEMKESGLEEVTNP
                     SQLFLGERPQRTTGSVVVSSIEGSRPILVELQALVSSTSYAMPKRMANGVDLNRVSLL
                     LAVMEKRLGMHLSGQDVYVNVVGGMHIDEPAIDLGIVAAVTSSLREVPVEPGLLVLGE
                     VGLGGEVRGISQAELRIREAAKMGFKRCLLPERNLAKLEPVDGMELVGIHEVGEALDV
                     VLA"
     gene            complement(1995..>2823)
                     /locus_tag="VFR82_14905"
     CDS             complement(1995..>2823)
                     /locus_tag="VFR82_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502938.1"
                     /translation="SLVLFLTVAGPVPAVEKIAAAVLVKDSLTAPHQQTTIETKLLAK
                     GLLKDSPLGGEPVELLVKGAVSATAMTGGDGRAFLSVLPKGKDIVPVQVRIGSSPRVS
                     SGEGEANLVVWERRTPILIVEMAALMEDVHAETPVSRLIPRIDPEARPIADAADELGK
                     LTQFYYGVIYVVIVPAGADVFATSAEARTWLSTHKFPRGFVLALSAGEDALGAKIDEL
                     HQAGWKSAKTGIGRSKAFAETFLRRRLEAIVVPEPPVGEVPRKSKVAKDWKDVRKKL"
BASE COUNT          588 a          887 c          756 g          592 t
ORIGIN      
        1 gaggatgctc ggaatacctc gagccgataa cgatctcctt cgtcgacaac cctggctgtt
       61 tcgctgccgt tgatggtgcc ggtcccgagc actccgctca ttgcccagac gcttagttgg
      121 aacggcctcg cgagtttgcc catctcgctc atgtcggaag gatgaccgga cagcacccga
      181 cccatcgtcg gcagtcgcaa cgtgaactga tcatccgtct ggagaaattc gaacaattca
      241 ctcccgatcg cggtaaagcc gcgtagacgc atcgcgctcg gacgtcggta atagaccgcc
      301 ccttccacgc gcgtggtaat gggaatgacg cctccgcgta ccttggcact gaacaagcct
      361 ttcatcgtcc gaaccgcaag ttcccgttgc tgcaaaagag ctgtcaactc gtctacggtg
      421 gccggcttca tgtctgcttc ctcacggtgc acgggagtcg tgcagccggc ggccacgagg
      481 cacagcaccg tcaggtaagg caccaggcgc acggcactct tacgccaata cgacatccag
      541 cgcttctccg acttcatgaa ttcccacgag ttccatcccg tcaaccggct cgagctttgc
      601 gaggttccgc tccggcaaca aacaccgttt gaaacccatt ttcgctgctt cacgtatccg
      661 taattcagcc tgactaatgc cgcgcacttc accgccaagg ccgacctcgc ccaagacgag
      721 caatccgggc tctaccggca cttcccgcaa gctcgaggtc actgccgcga cgataccgag
      781 atcgatcgcc ggttcatcaa tgtgcatgcc tcctaccaca ttcacataca catcttggcc
      841 ggacaaatgc atacccaatc gcttttccat gaccgccaat aggagcgaga cgcgattcag
      901 atccacgccg tttgccatcc gtttgggcat ggcgtaactg gtcgaagaca ccaacgcttg
      961 taattcgact agaatcggac gcgatccttc gatgctcgac acgaccacag agcctgtggt
     1021 ccgctgcggg cgttctccca aaaacaattg tgagggattg gtgacttctt caagcccgga
     1081 ctctttcatc tcgaacacac cgatctcatt ggtagatccg aaccgatttt ttacggcacg
     1141 gaggatgcga tagctgtgtc ccttgtctcc ttcgaaatac aggacggtat cgacgatgtg
     1201 ctccagcaag cgcggtccgg cgatcgctcc ctctttggtg acatgtccaa tgatgaatac
     1261 cggcacaccc gatcgcttgg cgaaccacat gagctggccg gacacttcct gaacctgact
     1321 gatgctgccg ggagccgacg tcaattgctc tgtgtagacc gtctgaatgg aatcgaccac
     1381 cacagcagca ggttgaattt cctgaacggc cttcaaaatc tgttcgagcg acgtttcggc
     1441 gaggaccagg aggttcggat gctcgacccc aagccgctgg ccacgcatct tgatctgccg
     1501 aggcgactct tccccggaga cgtagagcac cggctgcacg ttcgacgcca ggcgaggcag
     1561 ggcctgcaac agcaaggtgg tcttgccgat gcccggatct cctccgatca agataacggc
     1621 gcccggaatc actccgccgc ccaggactcg atcgaattcg ctgatgcgag tcagccggcg
     1681 gtcttcaccg atcacttcaa tcttcgcaat gggggtcgct gccggctgcg ccacgttcat
     1741 ggcaacgggg cgccctttcc ccgtcggggc atgccgttct tccttgatcg tgttccaccc
     1801 cccgcaatcc ggacagcgtc cgagccatcg tggcgattga tgcccgcatg cctggcagga
     1861 aaagatcgac ttggatttca tgaaccggaa agcctcaacg aggtggtgga acgagcttca
     1921 tcatagagga aagctgtgat caaaggaagg agatggatag gtcgacagcg cgagccagaa
     1981 gggcaccagt cctgctacaa tttttttcgc acgtccttcc agtctttggc aaccttgctt
     2041 ttccgcggca cctccccgac gggcggctcg ggtacgacaa tggcctcgag ccttcgcctg
     2101 aggaacgtct ccgcaaaggc tttcgaacga ccgatccccg tttttgccga cttccaacca
     2161 gcctgatgga gctcatcgat ttttgctccg agcgcgtcct cccccgctga tagggccaga
     2221 acgaaccccc gaggaaactt gtgggtggag agccaggtcc tcgcttccgc gctagtggca
     2281 aacacatcgg ctcctgcagg aacgatcacg acgtagatga caccgtaata gaactgagtg
     2341 agcttaccga gttcatcggc ggcatcggcg atcggccttg cttccgggtc gatgcgcggg
     2401 atcaaacgtg aaaccggcgt ctccgcatga acgtcctcca tcaatgcggc catttccact
     2461 atcagaatcg gggtgcggcg ttcccaaaca accagattcg cttcgccttc ccctgaggac
     2521 acgcgcgggc tgctgccgat tctcacttgg acggggacaa tgtccttgcc ttttgggaga
     2581 accgagagga atgccctccc atctcctccg gtcatcgccg ttgccgacac tgctcccttg
     2641 acgaggagtt caaccggctc tccgccaagt ggcgagtctt tcagtaaccc tttggccagc
     2701 aatttggttt cgatggtggt ctgttggtgg ggtgctgtaa ggctatcttt cacaagaact
     2761 gcggcggcga tcttttccac cgccggtaca ggaccggcga cggtcaggaa aagaaccaga
     2821 gac
//
LOCUS       DASTQY010000464         6671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_513244, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000464 DASTQY010000000
VERSION     DASTQY010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513244"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            465..905
                     /locus_tag="VFR82_14910"
     CDS             465..905
                     /locus_tag="VFR82_14910"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4502939.1"
                     /translation="MRFERGTGVPQNYREAARWYRLSAMHAYPDAQYKLCEMSEHGRG
                     LPQDYQEALRWCGLAADQGHGHAMFMLGRLYHTGHGVPRDVVRAHMWYNLASAYGYEE
                     GKRWRDRLAADDDSLQMTREQVAEAQKLAREWNVKMKATGHSGH"
     gene            1283..2629
                     /locus_tag="VFR82_14915"
     CDS             1283..2629
                     /locus_tag="VFR82_14915"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4502940.1"
                     /translation="MRTRTRIWLVLPWLFFFNSPPSVPSLLAEVVKPEALYDKSRALI
                     IGIEQYMQAPSVPGAVDEGKQVAQVLRQLGFDEIIELYNKDATSRRLHQALTDIFARK
                     VDRKGRVVVFFAGHTGTIRSSKGRDFGYLVPADAQVNKAAKSLTVETFRDFTRRSPSK
                     HTLLIINAPVRAWEAVALKSMSPQAEADSDASAVQVIARADQRGKADQAEGKTPFVGA
                     LLTGLSGAADLNQNGWLTASELGIYLKQQVYAISLRLDGEGDMVLFQQPTVEVGPVVE
                     PNSQATQDREAAKSEYEQAVALLQGGKYAEEALARLNRAIEYDPTFGDAYLLKSYLRL
                     EVLPDLDEALAAGQQAVKGAPDNPDAFYTLGLVHEKMGHYKEAEEAFIEAGKLNPENE
                     YVYFSLGTLYEDQLNDKAKSVAAFRRYLQLGGANARARSAVSQADQTVVSPADPSP"
     gene            3167..3559
                     /locus_tag="VFR82_14920"
     CDS             3167..3559
                     /locus_tag="VFR82_14920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4502941.1"
                     /translation="MLLVDDDVRVRQTLSSILKPYSNIEVVGEASDGDEAVTSVGNLR
                     PTVVIMDISMKKMDGITAARLIKARYPDVLVVGCSAHTQEYNIYAMKRVCAFEALLKK
                     EDATKDLYPAIQRVMAAGDRPAIAKDHN"
     gene            4287..4862
                     /gene="ybgF"
                     /locus_tag="VFR82_14925"
     CDS             4287..4862
                     /gene="ybgF"
                     /locus_tag="VFR82_14925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEU4502942.1"
                     /translation="MFVSFLLSPATKEATPTRIRAATLFWILFVSPVFCFFPQIGVAS
                     NVQDTSRHLYDRVMEEFKQGDYEAALAGFRFFIALHPKAALAANAQYWMGECQYRLGR
                     YEDALRSFSNVGAYDPVSQKRAASAFKIGQTYSMLGDYYRARFVFEEVIDHYPGGAEA
                     QLARKAIDGIIPKIGSKPSHPVKQVSNMTTR"
     gene            complement(5110..5775)
                     /locus_tag="VFR82_14930"
     CDS             complement(5110..5775)
                     /locus_tag="VFR82_14930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4502943.1"
                     /translation="MEDLILLHTDTISLLLVDDHPLVLESVSKLLEPHFTIVGKVQDS
                     GDIVSRALECRPDVILLDACMPGLSGFAATRELKKRLPKVKVILVTMLTEAISISEGF
                     RAGASGYVLKQSAAEELRLAIETVFANRRFLSEKIAPEIREALEHEWFRPEEYSSDLT
                     CRQREILVLLAQGQSTRHIAQQLNISTKTVEFHKANITRRLGVHTTSDLIKFALAHGL
                     TTL"
     gene            5980..6354
                     /locus_tag="VFR82_14935"
     CDS             5980..6354
                     /locus_tag="VFR82_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502944.1"
                     /translation="MWRAFLVSLSVLFMGPSIGSAVDFKVVDKNCWIEIFDDDEYDED
                     DPHVKIQGPAEYATLKDVYGRNWSKDIESVIVGTNATVHAWMNKDFTGPELTFTPGQR
                     VPKLSKLKMSNSIESMKIICGP"
BASE COUNT         1639 a         1697 c         1746 g         1589 t
ORIGIN      
        1 gcaggactgg gccaagtcgt tagttgctga gggttcgcag gtcgtccctt ctatccgctc
       61 gctacctctc cgcgcgcgaa acgcacagta cgtgtcgttt agccgcttgt ccaccgcaaa
      121 ttcatttgaa tgaatcgccg cccccacctc ggcgatgaac tgattccttc tggcgttgtt
      181 cagggtcttg cataaggtaa gtagggcaca tattcctctt gtgaaactat ccttgacacc
      241 tgtttggaga cgccctagct tgggcggtac ttatccatcc aagggaggcg gacatgacga
      301 cgcgctttcc actttttttc gtgggaataa tcctttgcat cctagtggcc tgctcggaac
      361 agcagccccg acctgacagt ctgcccgcag agaaagctca acagacagag acgctcctcg
      421 caaacgcgga gagtggaact ccagaggaca aatacaatct cggcatgcgg ttcgaaagag
      481 gaaccggcgt gccacagaac tatcgagaag ccgcccgctg gtatcgactc tctgcgatgc
      541 atgcgtatcc ggatgctcaa tacaaacttt gcgagatgtc cgagcatggg cgaggtttgc
      601 cccaggacta tcaagaggcg ttacgttggt gtggtctcgc cgccgatcag ggtcacgggc
      661 atgcgatgtt tatgcttgga cgtctttatc ataccgggca tggcgtgcct cgggatgttg
      721 tgagagcgca tatgtggtac aacctggcgt cagcttatgg ttatgaggag ggaaagagat
      781 ggcgtgatcg tcttgctgct gatgatgatt ctttgcagat gactcgcgaa caggttgctg
      841 aagcgcagaa gctggctcgt gaatggaatg tgaaaatgaa ggctaccggc cattcaggtc
      901 actagggcag ttttgtcgac accaaaccgc agcgccacgg tcatggtatg actactcgta
      961 acgatacact tcaactcttt ggtcaataca gtgaactatt ggctcaccct ttctttgcag
     1021 tttaccggtg gagtggataa ccctcttttc acgacgtcct cgaaacctcg ggcgtatttc
     1081 ttgaaaaccc atcacctact aagtagatga aagcagaata ggggccgtat tgaattgcga
     1141 agaaaaatcg cacagttgcg ttgttcgaat caccgtgtgg caattgccgg aatcttgccg
     1201 taagcgaggg aaacctctag gaccgcggta tttctagtcc cgatacagtg aagtagcggc
     1261 ccatggcagt aaggggactt ctatgcggac acgaacgcgt atctggcttg tcttgccatg
     1321 gttgttcttc ttcaattctc ccccctctgt cccaagcctt ctcgccgaag ttgtcaaacc
     1381 cgaagcgctc tatgacaaat ctcgggctct cattattggc atcgaacagt atatgcaggc
     1441 cccttctgtg cctggagcgg tcgacgaagg caagcaggtc gcgcaagtcc tccgtcagct
     1501 cggattcgac gagatcatcg agctgtataa caaagatgca acgtcacgac ggctccatca
     1561 agcgctcacc gacatcttcg cgagaaaagt ggaccgcaag ggccgggtgg tcgtgttctt
     1621 tgccggccat accggcacca ttcggagtag taaaggccgg gatttcggct atctcgttcc
     1681 cgcagatgct caggtcaaca aagccgcgaa gtcactcacc gtcgagacgt tcagagattt
     1741 caccaggcga tctccctcaa aacacaccct gctgattatc aatgcgcctg ttcgagcgtg
     1801 ggaggccgtt gctctcaagt ccatgtcgcc ccaagcagag gcggacagtg acgcgtccgc
     1861 cgttcaggtc attgcacgag ccgaccaacg tggaaaagcc gaccaggcag aaggcaagac
     1921 gccgtttgtc ggggccttat taaccggtct ctcaggagcg gctgatctca atcaaaatgg
     1981 atggctgact gcttctgaac taggtatcta cctcaaacaa caggtctatg ctatttcttt
     2041 gcgcctcgat ggcgagggag atatggtctt gtttcaacaa cctacagtgg aagtggggcc
     2101 cgtagtagag cccaattctc aggcgaccca ggaccgtgag gccgcgaaga gtgagtatga
     2161 acaggccgtc gcacttttac agggagggaa gtatgcggag gaggcattag ctcggctcaa
     2221 tcgtgccatc gaatatgatc caacctttgg cgacgcttac ctcttaaaaa gttatctccg
     2281 gctggaggtc ttaccagacc ttgacgaagc gcttgccgct gggcagcagg ctgtcaaagg
     2341 cgcgcctgac aatcctgacg cgttttatac gctcggcctg gtgcatgaaa aaatgggcca
     2401 ctacaaagaa gcggaggaag cgttcatcga ggcggggaag ctgaatccgg aaaatgagta
     2461 tgtgtatttt tcccttggaa cgctgtacga agatcaactc aacgacaagg ccaaatcagt
     2521 cgcagccttc cgccgatacc tccagttagg cggagccaat gctcgagcga gatcggctgt
     2581 gagtcaagcg gatcagacgg ttgtatctcc tgccgacccc tcgccttaaa cgttcaagtt
     2641 gtgtgaaacg ctgacacttt cagccccaat gccatattgc cccacaggag cagaagagag
     2701 accgcgccgg ctatgcatta actgttcgct ccggaccctc agaattccgc agagtgagag
     2761 acccatgcgt gtcgtcccct cccttcatca ttgagcctca gaagaagcag ctagctcatc
     2821 gatccgtcgc tgcattctcc tatggactga ttcgacacaa gcctggcgct tgtcagtttg
     2881 ataagtctcg acgaacgtct ctatttcctc gccagaaacg ttcatacgct taataatgaa
     2941 agcgtttagc gcattaacga gtatttcgtg aaaggctatc tcagcaacca ctacctgaaa
     3001 caagtgttca accttcatct ctcccccctt ttgtctcttt gcggtcaaaa atgccgggtt
     3061 ggaaccggat tcgtaaaaaa tgcagaatgt aggcgatcgc tgatagacaa atcgcgactc
     3121 tcttattata ttttgtcccc aaatgggaag tgcaaatcag atacgagtgc ttctcgtcga
     3181 tgatgacgtc cgtgtccgtc aaacactgag cagtatcctt aaaccgtatt ccaacattga
     3241 agtggtcggt gaagcgagcg atggcgacga ggccgttacg agtgttggga acctccggcc
     3301 gactgtagtg ataatggaca tcagtatgaa gaaaatggac ggcattacgg cagctcggtt
     3361 aatcaaggcg cgatatcctg atgttctcgt ggtgggatgc tctgcccaca cgcaagagta
     3421 caatatatat gccatgaagc gagtgtgtgc ttttgaagcg ttattgaaga aggaagatgc
     3481 gactaaggac ttgtaccccg cgatacagag agtcatggca gcgggtgatc gccctgctat
     3541 agccaaggat cacaactgaa ttagcctcga aaatagcgct gctcccaaat cacctacatc
     3601 gggcggaaac accgtaatcg tcagccgcct gccttgtccg ccgtttagag acggctgatc
     3661 aagcagaact gtatgaccat cgtgatggcc tcgcgaaacg ttcgtgccac cacttcttga
     3721 gtcaggtata aggccttcac tatagagtga acccggtata cggtttggaa ggaatttaag
     3781 accacatcag aagcggttcg accacaagag cgctggaaac tttcgaaggc atcgttcaac
     3841 aagacatcga agggggtttg gccgagacgt ttagcatcgt gatcggccgt ccttgtggtg
     3901 ttttcgcctt agacgtgcgc tttttacgac tggattggtg acaataacga catactcgcc
     3961 atcgggaatt gtgaagtgag acttgattat cagcatttct ctttgatctt aaccctggca
     4021 ctgcgattgc taagtagttt catgactcat tttaggtgta tgccaatacg gagggatctc
     4081 atgtatcagt tccgaagaga ggacatggcc ttacaggccg caatgcttct ggcggtctac
     4141 gagagaacgc cagttatggt gatggctcgc ggtggtatgg acctgtttag cattggtcca
     4201 atcaaccaag cagccgttca taaggccggc cgacatatag ctaccgtcta ccgtccagga
     4261 caggtagact atatgggatt agcgtgatgt tcgtgtcatt ccttctctca cctgcgacaa
     4321 aagaagcaac gccaactcgc attcgagccg ccacactgtt ctggattctt tttgtaagcc
     4381 ctgtcttctg tttctttcca caaatcggcg ttgcttccaa tgtgcaggat acttcgcgtc
     4441 atttgtatga tcgcgtgatg gaagaattca aacagggaga ctatgaagcg gcattggctg
     4501 gcttcaggtt ctttattgca ttacatccga aagcagcctt ggcggcgaac gcacaatact
     4561 ggatgggcga atgccaatac cggctaggtc gttatgaaga tgctctcagg tctttttcga
     4621 atgtcggagc gtatgaccca gtcagtcaaa agcgagctgc gtcggcattc aagatcggcc
     4681 agacctactc gatgcttggg gattattata gagcgcgctt cgtgttcgag gaggttattg
     4741 atcattaccc aggtggagcc gaagcccaac tcgctcgcaa ggcgattgat ggcattatcc
     4801 cgaagattgg ctctaagcct tcacatcctg tgaaacaggt cagcaacatg acgacgagat
     4861 aaccaagcat cggatttcag agaggaatcg ttcagcagga catcgaaagg ctcgggaagg
     4921 agattaagag cttatcgcta gacctgttac aaaacccacc gtctggctca caccttgtcg
     4981 tatagtctgc tttcgaaaag catgattagg acagctccac acgtaatact ctagaaggcc
     5041 gcgcgtgttc cgtcagatct tctgttacga aagtaaatgt tcacaggacc gcctgcgcgg
     5101 acctgtgact cagagcgtag tgagtccgtg cgccagagcg aacttgatga gatcagaagt
     5161 tgtatgaacc ccgagccttc gcgtgatatt tgctttgtga aactccaccg ttttggttga
     5221 gatattcagt tgttgagcaa tatggcgggt ggactgccct tgcgccagca agaccaaaat
     5281 ttcccgttgc ctgcacgtta aatcgctgga atactcctcg ggcctgaacc actcatgttc
     5341 caaggcctct cgtatttcag gcgcgatctt ctctgacagg aaacgtctgt tggcgaatac
     5401 ggtctcaatc gcgagacgca gttcttcagc tgctgactgc tttaacacgt agccgctcgc
     5461 gcctgctcga aacccttcgc tgatggaaat ggcttctgtc agcatcgtga cgagaattac
     5521 cttcactttt ggaagtcgct ttttcagttc tctggtagcc gcaaagccgc ttaaccccgg
     5581 catacaggca tccaagagga taacgtccgg tcgacactcc aaagcccgac tgacgatatc
     5641 gccggagtcc tgcaccttac caacaattgt gaaatggggt tcgagcagct tgctcacact
     5701 ttcaaggacg aggggatgat cgtcgaccag cagtaatgag atcgtatctg tatgcaacag
     5761 gattaaatcc tccaaggtac gtattatacc tcatctcgac gcatctagga accagcagca
     5821 gaggagaagt cggaactgaa ggctgggcgc attcctgttg ggggcttctc gcaccatcga
     5881 acagccattc gcacagagta gatcaacaag gtcaattccc agtaccgcat ccgcgtctcc
     5941 ggtcttagag tgaagtaaac atcaatggga ggaagaaata tgtggcgcgc cttcttggtc
     6001 agcctgagcg tcctcttcat gggcccctcg atagggtcgg ctgtggattt taaagtagta
     6061 gataagaatt gttggattga gattttcgat gacgacgaat atgacgagga cgatccgcac
     6121 gtcaagatcc aaggacccgc agagtacgcc accttgaaag atgtctatgg gcgcaattgg
     6181 agcaaggaca ttgaaagcgt aattgtgggc acaaatgcga ctgtgcacgc ctggatgaat
     6241 aaagacttta cggggccgga acttaccttt acccccggac aacgcgtgcc aaaactttcg
     6301 aaactcaaaa tgtcgaattc cattgaatcc atgaaaatta tctgcggacc ttaaccgacg
     6361 ctccattaag ttggtaacag tcttctcgca agagactctt aattcttaat atgggagcag
     6421 tcgggaccgc gaaatcatgg aatccgtgag gggctcaggt atctgaggga agtagccttc
     6481 gcttttgcga ccttgatgac cgaggacgca aaacggcagc taaccattcc cacttcgcaa
     6541 aacaggggat cgatcccgat gctatctaat ggtcggacta taaatcttca tcggtgtttg
     6601 ctaccgggct atttaccagc tccatctgtt gagagaggaa taacgagcgt cgggtttgcg
     6661 aacgccggcg g
//
LOCUS       DASTQY010000465         4111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1366007, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000465 DASTQY010000000
VERSION     DASTQY010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366007"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VFR82_14940"
     CDS             complement(<1..686)
                     /locus_tag="VFR82_14940"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU4502945.1"
                     /translation="MSAGNPSSIPLATYRLQLHRGFTFADAQRAVPYLHALGITDCYL
                     SPISKATPGSDHGYDVIDPVLLNPELGSEEEFQAFVRTVQQHGLGIVLDVVPNHMGIG
                     KTLNRWWRDVLENGPSSRYATAFDIDWHPIKRELENKVLLPILADQYGAVLESQEIEL
                     IYEDGVFFLRYYDHNLPLASKTWTRILSHRLDLLVADGEQAMPVLELQSILTALKNLP
                     GAAERDPERIT"
     gene            complement(683..1921)
                     /locus_tag="VFR82_14945"
     CDS             complement(683..1921)
                     /locus_tag="VFR82_14945"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002023.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU4502946.1"
                     /translation="MKPQRILGIIMAGGKGERLLPLTEVRSKPAVPFGGKYRIIDFVL
                     SNFVNSGITSLYVLVQYRSQSLIEHLRQSWQFGGRLAGSFVSVVPPQMRARDGWYEGT
                     ADAVYQNLNLIDDWDPDVIAVFGADHVYRMDIQQMIQAHLEHDADATVAALPVPIEAA
                     GGFGILEADGEGRMVGFEEKPTAPKPMPGRQGYALSSMGNYLFKTDTLVPLLKRDASR
                     PGPHDFGRNIVPELLEDHRVYVYNLHDNEIPGLRSYEEQGYWRDVGTLEAYWRANMDV
                     LGEHPPFDLRNTAWPILSSGYDGPMAALVRTHLDDSMIGQGSQCVDADIRKSIIGRGV
                     RIEQGAYLEECVILDDVHIGPHARLRRVIADRRSTVPAYSRIGYDTGHDRKRFHVSHS
                     GLVVLPHPSSANPHILRRAS"
     gene            2168..2980
                     /locus_tag="VFR82_14950"
     CDS             2168..2980
                     /locus_tag="VFR82_14950"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU4502947.1"
                     /translation="MFIWPLSGFLVLVLISATGYAAEESGGERAKEPPKAVEPKKKSE
                     GEVKSDSNGKPGSEKAGAEDKKTDDKKAEDKKADDKKAEDKKAEDKKAEDKKADEKKP
                     DEKKAEEPSPPKPEEHKKAVTSLNLAIKLALMADPQLFPLEIEVEMDKGKAVLTGAVP
                     TEEEKGRVAEIASKVEGVEGVVNKLNVTPALRDKLAKKQDETIAHVVRDRLSRSETLK
                     AVGFDVKSENGIVYLSGKTRFQVIALEAAEAARQVPGVRAVDTAGVQLVAKD"
     gene            3024..>4111
                     /locus_tag="VFR82_14955"
     CDS             3024..>4111
                     /locus_tag="VFR82_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4502948.1"
                     /translation="MHHTRPDPDLSLGLFMEDRLTRVIKDLSTHSRQHHRQHHIKRRW
                     LLSADFSYVWWGQVVSQVGDGISKLALLWFVYSVTGSPLKTSIIGILQTAPAIVLAPI
                     IGVAVDRLPKKALLIVSDLVRAVVIGLIPCWMSVDTFNVSSLYVLVTLHAVATAVFGP
                     ALTASVPSLVLPSQYTSANALLQITTSLGIILGPALSGLGIAALSSQEVLCLNALTYV
                     VSAACFIPIRFAPPVLSLVSHSPIMSAWRDVVEGLRYVLKGRRIIIFLTVTASLYTFA
                     TSAFTTLFPVFARKLLDLGPVEVGYLWSMLGIGLLTVSLALTVISAWSVKRRIKVIAM
                     ASAVSGLALLALVMVNHPTEAAGLLIVLG"
BASE COUNT          888 a         1163 c         1201 g          859 t
ORIGIN      
        1 gtaatccgtt cgggatcgcg ctcggcggca cccgggaggt tcttcaacgc ggtcagaatg
       61 ctttgaagct ccaacacggg catcgcctgt tccccatcgg cgaccaggag gtcgagccgg
      121 tgggacagga tgcgcgtcca cgtcttcgac gccagcggca ggttgtggtc gtaatagcgt
      181 aagaaaaaaa caccgtcttc gtagatcagc tcaatttcct ggctctcaag gactgcaccg
      241 tactgatcgg ccaggatcgg cagcaagacc ttgttttcca gttcgcgttt gatgggatgc
      301 caatcgatgt cgaaggccgt cgcgtaccgt gaactgggcc cgttctccag gacatccctc
      361 caccaccgat tcagcgtctt gccgattccc atgtggttgg gcaccacatc cagcacgatc
      421 ccgaggccgt gctgttggac cgtccggacg aatgcttgaa actcctcttc gcttccgagc
      481 tcgggattca ggaggacggg atcaatgaca tcgtatccat ggtcgctgcc gggcgtggct
      541 ttgctgatcg gtgacaaata gcaatcggtg attcccaagg catgcaagta gggcacggcc
      601 cgttgcgcat ccgcaaaggt gaacccgcga tgcaattgca gccggtaggt ggccagtggg
      661 atcgagctgg ggttgccagc gctcatgacg cgcgtctcaa tatgtgagga ttggcggacg
      721 aaggatgcgg aaggaccacg agacctgaat gcgaaacatg gaaccgtttc cggtcgtgtc
      781 ccgtgtcgta gccgatgcgg ctgtacgccg gaactgtgct gcgccggtct gcgatcactc
      841 gccgcaaccg cgcgtgagga ccgatatgca cgtcatccag aatgacacat tcttcgagat
      901 aggctccctg ttcgatccgc actccgcgtc cgatgatcga cttccggatg tcggcgtcaa
      961 cgcactgact gccctgcccg atcatcgaat catcgaggtg agtccggacc agagcagcca
     1021 tcggtccgtc atacccgctg gagagaatcg gccaggccgt attgcgtaag tcaaaaggag
     1081 gatgttcgcc gaggacatcc atgttcgctc gccaataggc ttccagagtc cccacatccc
     1141 gccagtatcc ctgttcttcg tacgaccgca gtccgggaat ttcgttatcg tgaaggttgt
     1201 acacgtacac ccgatgatct tccagcaatt ccggaacgat attgcgtcca aagtcatgcg
     1261 ggcctggtcg cgaggcatcg cgcttcagca atggaaccaa cgtgtcggtc ttgaacaaat
     1321 agttacccat tgaagacaac gcgtacccct gccgtcccgg catgggcttc ggcgcagtcg
     1381 gcttttcctc aaatccgacc attcgtccct ctccgtccgc ttccaggatc ccgaacccgc
     1441 ccgccgcttc gatcggaaca ggcaaggcgg cgactgtcgc gtcggcatcg tgttcgagat
     1501 gcgcttggat catctgctgg atgtccatac gatacacgtg gtcggcaccg aacacggcga
     1561 tgacgtcagg gtcccaatcg tcgatgaggt tgagattttg gtacacggca tctgcggtgc
     1621 cttcatacca tccgtcccgt gcgcgcattt gcggagggac cacgctcacg aagctgccgg
     1681 cgagacgccc gccgaactgc cagctctgac ggagatgttc gataagggac tgagaccggt
     1741 attgtacgag gacgtacagg gatgtgattc cggaattgac gaaattgctg aggacgaaat
     1801 cgatgatacg gtatttgcct ccgaacggca cggccggctt gctgcggact tccgtcaatg
     1861 gcaagagacg ctcccccttc cctcccgcca tgatgattcc caaaatccgt tgcggcttca
     1921 aatggttccc ctttatttcg tcgcacggaa gtgtgggggc aacaggaacg ataccaactt
     1981 gctgtgggag cacctaacta ggagatcccc cggtttcatg tggttttgca tgcttatcgg
     2041 tccgtgacac ggcggactgg ggaattcccc agtacgcttc actgggtctt accggcgaag
     2101 atgcaacgga ctcatgggtt tcccttggtg tgcacagacg gacctacagg ttggcagggt
     2161 cggtcacatg ttcatctggc ccctctctgg atttctagta ttggttctga tcagtgcaac
     2221 gggctatgcg gctgaggaga gcggaggcga gcgggcaaag gaacccccca aggcggttga
     2281 accgaagaaa aagtcagagg gagaggtaaa gtccgacagc aatggaaagc ccgggtctga
     2341 aaaggccgga gccgaggaca aaaagacgga cgacaagaaa gcagaagaca aaaaggcgga
     2401 cgacaaaaaa gcagaggaca aaaaggctga agacaaaaag gctgaagaca agaaagcgga
     2461 cgagaagaaa ccggacgaga aaaaggcaga agaaccgtcc cccccgaaac cagaggagca
     2521 caagaaggct gtcacgtcgc tgaacctggc catcaaattg gcgctgatgg ccgatccgca
     2581 attgtttccc cttgaaatcg aagtggaaat ggataaaggc aaggccgtgc tgaccggtgc
     2641 tgtcccaact gaagaggaaa aaggaagagt ggccgagatc gccagcaaag tcgaaggagt
     2701 agaaggggtc gtgaacaagc tgaacgtgac gccggcgctc cgggacaaac tggccaaaaa
     2761 gcaagacgag acgattgccc atgtcgttcg agatcgtctg tcgcgtagcg aaactctgaa
     2821 ggcggtcgga ttcgacgtga aatccgagaa cggcattgtg tatctgagcg gcaaaacgcg
     2881 attccaagtc atcgcgctcg aagcggcgga ggccgcaagg caggtccctg gcgttcgcgc
     2941 cgtcgatact gccggtgtgc agctcgtcgc aaaagactaa catcagaggt cgtcatcgag
     3001 cccaagatcc gagtcaagcg cacgtgcacc acacgcggcc tgacccggac ctgagcctcg
     3061 gcctgttcat ggaggatcgg ctcacgcgtg tcatcaaaga cttatcgacg cattcccgcc
     3121 agcatcaccg tcagcatcac atcaagcggc ggtggttgct tagcgccgat ttctcgtacg
     3181 tgtggtgggg ccaggtcgta tcgcaagtag gagacggtat ctccaagctg gcgttgctct
     3241 ggtttgtgta ttcggtcacg ggctcaccgc tgaaaaccag catcatcgga atcttacaaa
     3301 ccgctcccgc tattgttctg gcgcccatca tcggggtggc cgtcgaccgc cttccgaaaa
     3361 aagcactgtt gatcgtctcg gatctcgtgc gggcggtggt gatcgggctc attccgtgct
     3421 ggatgtcggt ggacaccttt aacgtctctt ccttatatgt cctcgtgact ttgcatgcag
     3481 tggccacggc agtgtttgga ccggcgttga ctgcatccgt tccctcattg gtactgccgt
     3541 ctcagtacac ctccgccaac gccctgcttc agatcaccac cagtttgggc atcattttgg
     3601 gtccagcctt gagcggatta gggatcgccg cgctcagctc acaggaagtg ttatgcctta
     3661 atgctttgac atacgtggtt tcggctgcct gcttcattcc aatccggttc gcgcctcccg
     3721 tgctcagcct ggtgtctcat tctcccatca tgtctgcctg gcgcgacgtc gtcgaaggac
     3781 ttcggtatgt gctgaaaggc cgccggatca tcatctttct caccgtcact gcctcactgt
     3841 acacgtttgc cacgagcgca tttaccacgc tgttccccgt attcgccagg aaactgctgg
     3901 atctcggtcc ggtggaagta ggctatttgt ggtccatgct cggaatagga ctgctcaccg
     3961 tgtcgctcgc actgaccgtc atcagcgcct ggagcgtgaa gcgccggatc aaggtgattg
     4021 ccatggcgag cgccgtgagc ggtctcgccc ttctggccct ggtcatggtg aatcatccga
     4081 ctgaggcggc tggtctgttg atcgtcttgg g
//
LOCUS       DASTQY010000466        14969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1522297, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000466 DASTQY010000000
VERSION     DASTQY010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 14969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14969
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522297"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2410)
                     /locus_tag="VFR82_14960"
     CDS             complement(<1..2410)
                     /locus_tag="VFR82_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502949.1"
                     /translation="MAKSRGDSKLAVAGALTLVLAIAGVLLVKEPLRSSRPVGNGLEI
                     KQSTAEQFVRARLWEDPVAAVDRAIREQGAAKTTSVGFSLAKRLGPLRHAIGQRVHRG
                     EQLTVLLITKTGGGYVESTESRLRDRYAVGTALGAACYAPEDEGHLSFVHWEPNSSVQ
                     GLPYEWYRLRKTRVCGPAGSRADSILIVWLPDEAITAGFFATLTSLSQGLVCREDGSN
                     ECLLNGAKPSLVRLDPALQRAVSFKIVGPRSSSAYRALLDEAGALYGNAQHDVGIWPT
                     ADGWVELYSPWASAMKGLLAYGLKSESGSSCTSYEECEHRFYRQLSEAKVRVIYDVGS
                     DEIRFQSLIAELERRQVRLGWDAVILIGEWDSFYGRTLPIEFRAAACAKIATFSETDL
                     KAIQVPVQVKQWCQTVAQAIDLQIQRPADYESLLLNVFRYSYLSGLDGEIPGDDAARA
                     ARAAKAGADAKGKDATRDRPEGTGQTDYVRALVARIHDEGEGARAIGILGTDPYDALL
                     IIKALRPAFPHAIFFTVDLDARHLHPSEYKSTRNMVIAAPFGLQLEGGLQRDVPPFRS
                     SYQTSAYFAVLQAVGHVRCEQQGHQQQTGPCTTSYHVAQTPDNRLYDTASHPRLFEVG
                     REGAVDLSVVEKEGVRTVHPLRADLDYTDEHGQLKQGIEFDDHAAATLLAIGVVLATI
                     IVWSNQRLWLWVSRNPVWTGALTIVLLAAFWAVVLTAGGSVLLAGHDEGEPFSWTAGV
                     SIWPGELIRLFVVVLSLALLIKGSRDLMRNSDLISRDFGLEREAADRRFSLRTFWTNL
                     KRVFH"
     gene            complement(2603..4078)
                     /locus_tag="VFR82_14965"
     CDS             complement(2603..4078)
                     /locus_tag="VFR82_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEU4502950.1"
                     /translation="MGDRHLETLTFNNTYARLPEAFYAKLNPTPFSSSPYLVSVNAAA
                     ADLIDLDLREAKRPEFAALFGGSMLTPAMEPLAMLYSGHQFGVYVPQLGDGRAILLGE
                     TINARGERWDLHLKGAGITPFSRDGDGRAVLRSTIREYLCSEAMHALGIPSTRALSIV
                     GSDDKVYREQVESGAMLVRMAPSHVRFGTFEIFYYRRQHEHLKVLADYVIDHHYPHLR
                     ETTDKYAALFAQVVERTARLIARWQAVGWAHGVMNTDNMSILGLTLDYGPFGFMDDYD
                     AGFICNHSDHNGRYAFNQQPYIGLWNLSCLAQALLPLAEKDALKEGLDGYTPLFEREY
                     RSLMRAKLGLAEARDQDEELIRDFLGLLQGSHADYTIVLRELGGFLSGRGMVNDRLRE
                     HFLNRERFDAWAAQYAERLRAERSRDDDRQQAMNRVNPKYVLRNYLAQAAIEKAQQKD
                     FAEIDRLLALLQHPYTDQPGMEGYAAPPPNWGKHVSVSCSS"
     gene            complement(4338..4985)
                     /locus_tag="VFR82_14970"
     CDS             complement(4338..4985)
                     /locus_tag="VFR82_14970"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU4502951.1"
                     /translation="MEKLVKGFLKFHGEVFGRKKDLFIRLSQKQEPRALFITCSDSRV
                     DPTLMTQAEPGELFILRNAGNMVPPYGSMQGGSTATIEYAMAVLKVPHIIVCGHTDCA
                     VMKALLHPEEVQDLPAVREWVGQAETTRRLMREHYTDLEGNDRLIKTTQENVRAQLDH
                     LRTHPSVALQLRKGKVDLHGWVYSILTGDLWVYDFDDEQFTPLVNEAPPRLKAAR"
     gene            complement(5034..5450)
                     /locus_tag="VFR82_14975"
     CDS             complement(5034..5450)
                     /locus_tag="VFR82_14975"
                     /inference="COORDINATES: protein motif:HMM:NF021568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HEU4502952.1"
                     /translation="MARQTAHDLLVHDSVQLYNPAGSTMETAIRNAIIKFEQEFMGRG
                     PDDVRASIVRDLVVVRLKGVLTPAERQLAKTAEGVDMVKRLRQNLIAQGRDKLCEQVS
                     EITGAKILGVFTDIDVQLGERVFVFTMDRELQNGGR"
     gene            5738..7435
                     /locus_tag="VFR82_14980"
     CDS             5738..7435
                     /locus_tag="VFR82_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU4502953.1"
                     /translation="MTAALLVPLFPLLTVLLVVAGNEATRRQRSKIGAWPLAAAFLGA
                     IFTLYVVATSGPISIRFYEPAEGSALPVPIGFHIDRLSAVMMVLISAIGTIIYTFSLD
                     YMYQEPHERRYFATLGVAVCVLLCMVSSGNLMMLFVFWQILGYLLYVLIHNHAHAGTL
                     EGAFRTFTILRVGDIAFLAAIALAYSLYGTLEFPDIFAAAARSTATVSLLPGIELNGA
                     TAVALLLFIGGMSKSAQFPFHVWLPRYLYAPTPVTALLHAGIINAGGFLINRLAPLFG
                     LSSTTLHIAFLIGTLTAVLGASMMLAQNDIKNMLGFSTIGQMGYMVMECGLGAFSLAV
                     FHLIAHGLFKATVFLNSGNVIGRARQEPHFPHASHDAGEEGYSPLTWFTGFFTTLVIP
                     LLILLLTHGILHIPLLEAQGTVIFLFFIWVTSSQAILTLTRLRAVASWKVSAAMLLTL
                     LFVVFMYLFAVESFTAFLYPDPQEVTFYFKAAELPGRLFDMMIGATTLVIVIGWCYVY
                     MKAHGRSLPLPTWIESIRIKLYVLFLNRLYVDEGGQRLEQARLSLFQRLEKHAEERV"
     gene            7565..9265
                     /locus_tag="VFR82_14985"
     CDS             7565..9265
                     /locus_tag="VFR82_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU4502954.1"
                     /translation="MTLTLLVPLLLLVASGLVLIGSDSSRHARAKIAAYPIGVAFLAA
                     MATLFQVTTQGPLALQFYDLSSVGSFAIPIGFYIDRLSAVMMTLITGVSVIIYTYSIG
                     YMYQDAHARRYLAMICLTDFVLICMVASSNLMMLFLFWQVLSYLLYVLAHNHAHAGTL
                     TGAFKTFTVLRVADTAFLAGIALAYQLYGTLEFQDLFLRAAETQIGLPIGFGMEVSGA
                     TAVTFLFFVGAMGKSAQFPIHFWLPGSLFAPTPVHALLHAGIINAGGFLINRLAPLFA
                     LSSTTLHVAFVIGTLTAFLGATMMLAQNDIKKTLGFSTIGQMGYMIMECGLGAFSLAV
                     FHLIAHGLFKATVFLNCGNVIHKARQEPHFPHIDHQREEEGLSRLTWVTGFVTTLLIP
                     LFILLLTHGVLRIPLLESQGTVIFLFFIWITSSQAILTLTRLRQVASWKVSAAMLMTL
                     LFIVFVYLFAVESFTAFLYPDPDAVASYFRAADLPDWLFDFIILAATILMIASWSYLY
                     MTAHGRTVRMPSWIDDMRIRLYLPFMNRLYADELHQLLGDTLMRLVHQFDKRERGWSR
                     "
     gene            9262..10572
                     /locus_tag="VFR82_14990"
     CDS             9262..10572
                     /locus_tag="VFR82_14990"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU4502955.1"
                     /translation="MTDGALLSWLLAGIPLLGALGSLLFWPNADRLKFFSILWSAVSC
                     ASIIGFAGYLAMPPEGFLPLYLLPLTAVISLLAQPAHDHYRQSCLMTLVCLGLGLTVL
                     TSSPPVGNLALALLLVLVASLLYRHHSALWPMSWWGIGTYGLGVLCALLAVAAGPPVS
                     SVASLVTCAVLLPLVPFHDGHLTALTRLPGNLPAFIVVLLPIAGLHELPHVLPTVPDV
                     VAWTVSFFALLGALYGAVKALAQSRVRLLLAYGSLSFFSMLWWFVAATRTATPRAALM
                     AGTVGLATSGLLVAWQVVRTRYGDDIDPQAVSGLASGMPQYAVLLSLLALAAMGLPPF
                     GVFAGFMGLLLNAPFPSTVGLFITLLAWLAASWYIMQMVQRLLYGTRRQELRYTDVLK
                     TELASLVMAVLVLLALGLAPATLFAPGQTGSERVSVKESSTWNR"
     gene            10560..13760
                     /locus_tag="VFR82_14995"
     CDS             10560..13760
                     /locus_tag="VFR82_14995"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HEU4502956.1"
                     /translation="MEQVDRTIDIQTRRMELRGIVKLAGEVIAQYWPMRTFVHHNPLH
                     SLEYLPFEETVRRGKQFMGGNGYLPGRTYRQYLRSGRIQLRHLDEALSSRVQGKEVVV
                     GSRVVTHGEVLRGCLTEGLCAAVVEPLDNQLPDDSLGVVERLAGRLESVLTVPDLAER
                     IRGIVDSDEDALGRWLTLSHWCDDTLGTHMVGQINDQMIKWCEAFLDEGHATWAMPER
                     EKGFYKAWKDIASHEWSPCGIADSKRKIAQLPDYPEDALLEHLDALGIPARLRQDYLS
                     LQLTALPGWAGFIKWREEERDYPWQQAYPVGLVKYLAIRLWYARELVHKACSVHLGIE
                     GRYDAVVAYMRSHAEEYYLRRQRVAGRLPALYAEEVDRLAHQKGGGWKTVLDRYQAEV
                     VPRQEAATRRGAARKLLALGRCLEIDPKQLVETDPETLQQLVAWLEAFPESDHGPVWL
                     KAFEAGYQERLLGELRAKSREADLPNAPRPYSQSVYCIDVRSEPFRRHLESTGPNETY
                     GFAGFFAAFIRYRAWGKEHDTEQFPVIMRAKNEVREIPRSYLDHRVSKHKARAKWVRA
                     GHTLLHDLKENVVTPYVMVESLGWFYSLPIFGKTLVPSLYRRWTAWLRRLFVPSIATT
                     LTVDKLAPQDTAEMLIADQQALVRKALQERLGLRASRITPALVEGLRQRALTGSSEQD
                     EAVIEAANRAGLSADALQSFVEILRREYDLNARSASRQKERITRTGFTLEEQALTVDT
                     ALRMMGLTKTFARLVLFCSHGSTSDNNPYESALDCGACGGNEGKPNARVLAMMANNQK
                     VRERLSKGGIDIPGDTHFLAGQMDTTTDEVQLFDLEDVPPTHRADLARLQEDLREASA
                     LTSLERCARFPDVPRSLEEKQAETHVRRRSADWSQVRPEWGLSGNTAFLIGRRELTKG
                     LNLEGRVFLHSYDYREDPTNRLLEVLLTAPQVVGQWINMEHYFSAVDNDVYGSGSKIY
                     HNVVGRLGIMSGPWSDLRLGLARQTVMNGSMPYHEPMRLLTLIEAPRERIEKLISRHE
                     VLQNYYHNEWVHLVALDPEDNVWYRYHPKGIWHVIP"
     gene            13823..14158
                     /locus_tag="VFR82_15000"
     CDS             13823..14158
                     /locus_tag="VFR82_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEU4502957.1"
                     /translation="MGLILHPMKEIRVIVAGEQRAFVTDLLDRVNATGYTIIGNVSGK
                     GHHGVREAHFMFSEQESLEMIMTVVPQEKVEPILAGLRPLFERHSGVMFVADVAVSRQ
                     EYFAKSKPA"
     gene            14251..14520
                     /locus_tag="VFR82_15005"
     CDS             14251..14520
                     /locus_tag="VFR82_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502958.1"
                     /translation="MKGLRFERIGQGRYYDVVFHLGSTYVPVSDETIEELKAQSLLPA
                     ERFLDLLIDRVGYSSYLKDQIRKELKSSGDPVTQITVLQGAIREL"
BASE COUNT         2882 a         4466 c         4283 g         3338 t
ORIGIN      
        1 gatgaaagac ccgcttcaga ttcgtccaga aggtcctgag cgagaaccgc cggtcagcgg
       61 cctcccgctc aagtccaaaa tcacggctga tcaggtcact gttcctcatc agatcacggg
      121 agcctttgat gagcagtgcg agcgagagca cgacgacgaa gagccgaatc agttcgccgg
      181 gccagatact gacgccagcc gtccatgaaa acggttctcc ttcgtcatgc ccggcaagca
      241 gcacagatcc accggcggtg agcacaacag cccagaacgc cgccaggagc acaatggtca
      301 gcgctccagt ccatacgggg tttcgcgata cccacagcca cagccgctgg ttgctccaca
      361 cgatgattgt ggcgagcacc accccgatgg cgagaagcgt ggcagccgcg tggtcatcga
      421 attcaatgcc ttgtttcagt tgtccatgtt cgtccgtgta gtcgagatcc gcgcgcaacg
      481 gatggacggt ccgcacgcct tccttttcga ccacactcaa gtcaacggcg ccctctcttc
      541 cgacttcgaa gagtcgcgga tgggaggcag tgtcatagag gcgattgtcc ggggtctgcg
      601 cgacatggta gctggtcgtg cagggcccgg tttgctgttg atgtccttgt tgctcgcagc
      661 gaacgtgacc cacggcttgc aacacggcga agtacgctga agtttggtaa ctgctgcgga
      721 agggcggaac gtcccgttga agaccgccct ccagctgcaa cccgaagggg gcggcgatga
      781 ccatgttgcg cgtggacttg tattcgctgg gatgcaagtg gcgcgcgtcc agatcaacgg
      841 taaaaaagat cgcatgagga aacgcggggc gcagcgcctt gatgatcagc agcgcgtcat
      901 aggggtctgt gcccagaatg ccgatcgcac gggccccttc cccttcatcg tgaatgcgcg
      961 cgaccagcgc gcgcacatag tcagtttgcc ccgtgccttc cggccggtcc cgcgttgcat
     1021 cctttccttt ggcgtcggcg cccgccttgg cggcgcgtgc cgctctggca gcatcgtcgc
     1081 ctgggatttc gccgtccaat ccgctcaaat aactgtatcg gaacacgttg aggagcagcg
     1141 actcgtagtc ggcgggacgt tggatctgca gatcgatcgc ctgggcaacc gtctggcacc
     1201 attgtttgac ctgaaccggc acctgaatgg ccttaagatc ggtttccgag aacgtcgcaa
     1261 ttttcgcgca ggccgcggcg cgaaattcaa tcggaagggt gcgcccataa aacgaatccc
     1321 attcaccgat aagaatcacg gcgtcccagc ccagccggac ctgtcgtcgt tccaattcgg
     1381 caatcaacga ttgaaagcgt atctcgtccg acccgacgtc gtagatgact ctgaccttgg
     1441 cctccgatag ttggcgataa aaccggtgct cacattcttc gtagcttgta caggatgagc
     1501 ctgactcgga cttgaggcca taggccaaaa ggcctttcat cgcactggcc caaggggagt
     1561 aaagctcgac ccaaccgtcc gcagtgggcc agatcccaac gtcgtgctgc gcgtttccgt
     1621 acaatgcacc cgcttcatcg agaagcgccc gatatgccga tgagctgcga ggaccgacga
     1681 tcttgaagga gacggctcgc tgcagggccg gatccaggcg cacgagagaa ggtttcgcac
     1741 cgttgagcag acactcgttg ctgccgtctt cacgacacac gagcccttgc gacaacgatg
     1801 ttaaggtggc gaagaagccg gcggtgatcg cttcgtcggg taaccatacg atgaggatgc
     1861 tgtcggcgcg ggagcctgca ggaccgcaga cgcgcgtctt tcgaagacga taccattcat
     1921 agggaagccc ctgaactgag ctgttcggtt cccaatgcac gaaggacaga tgaccctcgt
     1981 cttccggcgc atagcaggcg gctccgagag ccgtgccgac ggcataccga tcccgaaggc
     2041 gcgattcggt actctcgaca tagcctcccc cggtcttcgt gatgagcaaa accgtaagtt
     2101 gctctccacg atgtactcgc tggccaatcg catgacgcag gggtcccagt cgtttggcca
     2161 aggaaaatcc tacggatgtg gtcttggcgg ctccttgctc gcgaatcgcc cggtcaacgg
     2221 cagccactgg gtcttcccac agtcgcgccc tgacgaactg ctcggcagtg gactgcttga
     2281 tctccagtcc gttgcctacg ggtcgtgagc tgcgcaaggg ctctttgacc aacaacaccc
     2341 cggcaatggc cagcaccagc gtgagtgcgc ccgcgaccgc taattttgaa tcacctcgag
     2401 acttggccac ggatggcttc tcctcattcg cgcaaaaatg ggaccactgt acaccagaca
     2461 atgaggggga ctgcaacaag gcactgcatt gctctgcacc tgagcgggag cggcttcatc
     2521 tctatacgac cggatgtgtg aacccacctt ggaaagaccg tggggaatat gcacagctgg
     2581 ggtaactgag gaacggccgc acttacgagg agcagctcac gctgacgtgc ttgccccaat
     2641 taggcggagg ggcggcatat ccctccattc caggctggtc agtatagggg tgttggagaa
     2701 gggccaggag acggtcgatc tcagcgaagt ccttttgctg agccttttca atggctgctt
     2761 gggccaggta attgcggagc acatatttcg gattcacgcg gttcatggct tgttgcctgt
     2821 cgtcatcccg actacgctcc gctcgcagcc gctcggcata ctgagcggcc catgcatcga
     2881 agcgttctct gttgagaaaa tgttcgcgca gcctgtcatt gaccattcct cgtccggaga
     2941 gaaacccgcc caattctctc aacacgatgg tatagtccgc atggctgcct tgcagcagac
     3001 ccaagaaatc acggatgagt tcctcgtctt ggtctcgtgc ttctgccaac ccgagcttgg
     3061 cccgcatgag tgatcggtac tcccgctcga agagcggcgt atagccatcg agtccttcct
     3121 ttaaggcgtc cttctccgcg agcggcagca aagcctgagc aagacagctc agattccaca
     3181 atccgatata cggctgctgg ttgaaagcat accggccgtt gtgatcggag tgattgcaga
     3241 tgaatcccgc atcatagtca tccatgaagc cgaagggacc ataatcgagg gtcaggccaa
     3301 ggatggacat gttgtcggtg ttcatcacgc cgtgcgccca gccaactgct tgccaccgag
     3361 caatcaacct ggccgtgcgt tccacgactt gcgcaaacaa agccgcatat ttgtccgtgg
     3421 tctctcgaag gtgagggtaa tgatgatcga tgacgtaatc ggccaagacc ttgagatgtt
     3481 cgtgttggcg ccgataatag aagatctcga aagtgccgaa tcgcacatgc gagggggcca
     3541 tacgcacgag catcgcgccg ctttcgacct gttcccggta cactttatcg tcgctgccga
     3601 caatggagag cgctctcgtt gagggaatgc cgagcgcatg catggcctcg ctgcacaggt
     3661 actcccgtat cgtcgaacgc agcactgcac ggccgtcgcc gtccctggag aagggcgtga
     3721 tccctgcccc tttgagatgc agatcccacc gctcaccccg tgcattgatg gtttcaccca
     3781 gaaggatggc gcgaccgtct ccaagctgcg gcacgtagac gccaaattga tgacccgaat
     3841 acagcatggc aagcggctcc atagccggcg tcaacatact gcccccgaaa agggcggcga
     3901 actccggcct cttcgcttct cgaagatcga gatcgatgag atcggcggcg gcagcgttga
     3961 cactgaccag ataaggcgat gacgagaacg gggtggggtt cagtttcgcg tagaacgcct
     4021 ctgggagacg ggcatacgtg ttgttaaacg taagggtttc gagatgacga tcgcccatgg
     4081 acccaggatg ccagggccga ctggctgttt acaactagag aaccttgggc aagtctggac
     4141 cgatgtacgt cctgggtgct cacaaagtgt gtcccgtaag gccacagcga gggcccgcga
     4201 cacaaagaat aatgagcgtc cacgttgcgg acgggcgcga ttgctgagcg cccagtgtcc
     4261 cccaaggcga agcgcctggc acgtttggcc ctcggcgcga tgcgagaacg atgcggtcgg
     4321 attgtttcag cagcctgcta acgggcggct ttcaagcgcg ggggcgcctc gttcacgagc
     4381 ggcgtgaatt gctcgtcgtc gaagtcgtag acccaaaggt caccggtcag aatggagtac
     4441 acccaaccgt ggagatcgac cttcccctta cggagctgca aggcgacgga gggatgcgtt
     4501 ctgagatggt ctaactgggc gcggacgttt tcctgcgtcg ttttaatcaa acggtcattg
     4561 ccttcgaggt cggtataatg ctcacgcatc aagcgtcttg tggtctccgc ctgcccgacc
     4621 cattccctca cggcaggcaa atcctggacc tcttctggat gcaacaacgc cttcatgacg
     4681 gcgcaatcgg tgtgaccgca cacaatgatg tgcggcactt tgagtacggc catcgcatac
     4741 tcgatcgtcg cggtgctccc tccttgcatc gatccgtagg gcggaaccat gttgccggca
     4801 ttccttaaaa tgaagagctc ccccggctcg gcctgagtca tcagcgtagg atccacacga
     4861 gaatccgaac aggtgataaa cagggcgcgg ggttcctgtt tttgcgacaa acggataaag
     4921 agatctttct tccttccgaa gacctcgccg tggaatttca aaaatccttt aacgagtttt
     4981 tccatagcta tatgccccgc tctcctatca ccgcacgttc aagcacgccg ctgtcaacgt
     5041 ccgccgttct gcagctcacg gtccatcgtg aagacgaaga cccgttcacc gagttgcaca
     5101 tcaatgtccg tgaagacgcc caaaatcttg gcaccggtga tctcgctcac ctgctcgcag
     5161 agcttatcgc gtccttgcgc gattaaattc tgccgcagac gcttcaccat gtcgacacct
     5221 tccgcagttt ttgccaactg ccgttccgcc ggcgtcagga cccccttgag gcgcacgacc
     5281 actaaatcac ggacgatact ggcccggacg tcgtccgggc ccctgcccat gaactcctgc
     5341 tcgaacttga tgatcgcgtt gcggatcgcg gtttccatcg ttgaacctgc gggattatag
     5401 agttgcacag aatcatgaac aagtagatcg tgagccgttt gtctcgccat cctggtctgc
     5461 tgtgggcctt tagtgctatc cggcaagggt attgagaccc gaggtcgact gctatgttga
     5521 cggcgttcgc cccagcatat tatttatgat gagacggcgc tgctgcctgt ccaaagttcg
     5581 tgaaggacga acaggacgcc ggattactcc aataggccgt agggtgaccg gctatcagac
     5641 agaaaggccg gtcgcagtac aagccaaccg aatggcatcg acgtctgtcg aggaatcggt
     5701 tggcttttct tttgtttcac ggagtgatga gacaccgatg accgccgctc tgcttgtccc
     5761 actgtttccc cttttgacag tgctcctcgt cgtcgccggc aacgaggcga ccaggcgcca
     5821 acgatcgaaa atcggcgcct ggccgctcgc cgccgccttc ttgggcgcca tcttcactct
     5881 ctacgtcgtg gcgaccagcg ggccgatttc aatccggttc tacgagcccg cggaaggcag
     5941 cgcgcttccc gtaccgattg gcttccatat cgatcgtctg agtgccgtga tgatggtgtt
     6001 gatctccgcc atcgggacca tcatttacac attctccctc gactacatgt atcaggaacc
     6061 tcacgagcgc cggtatttcg ccaccctcgg cgttgcagtc tgcgtgctcc tttgcatggt
     6121 ctcaagcggc aacctgatga tgctcttcgt cttctggcag atcctcggct atctgctcta
     6181 tgtcctgatc cacaaccatg cccatgccgg gaccctggaa ggcgcctttc gcacgttcac
     6241 catcttgcgg gttggtgaca tcgcatttct cgcggctatc gccttggcct actcactgta
     6301 tggcacgttg gaatttccgg acatctttgc cgccgcggcg cgatcgacgg ctaccgtatc
     6361 gcttctaccg ggcattgaac ttaacggcgc cacggcggtc gcgttgcttc ttttcatcgg
     6421 cggcatgagc aagtccgctc aatttccttt tcacgtctgg cttccccggt acctctatgc
     6481 gccaaccccc gtgacggcgc tgttgcacgc gggcatcatc aacgccggag gctttctgat
     6541 caaccggctt gcgccgctgt ttggcttaag ttccacaact ctgcacatcg catttctcat
     6601 cggcacgctg actgcggtac tcggagcctc catgatgctg gcgcagaacg acatcaagaa
     6661 catgcttggg ttttcgacga tcggccagat gggctacatg gtgatggagt gcgggctggg
     6721 ggctttttct ttggccgtat tccatttgat tgcgcacgga ttgttcaagg ccaccgtgtt
     6781 cttgaacagc ggcaatgtca tcggcagagc gcgtcaagaa ccgcatttcc cccacgcgag
     6841 ccatgacgca ggcgaagaag gttactcgcc cctgacatgg ttcactggtt tctttacgac
     6901 tctggtgatt ccgctgctga ttctcttgct cacccacggc atcctgcaca ttcctctgct
     6961 cgaagcgcaa ggcacggtga tcttcctctt tttcatttgg gtcacgtcgt ctcaggccat
     7021 cctcaccctg acccgcttgc gcgccgtggc ttcgtggaaa gtatcggccg cgatgctgct
     7081 gacgctcttg ttcgtcgtct tcatgtacct cttcgccgta gagtcgttca ctgccttctt
     7141 atatccggat ccccaggagg tcaccttcta ctttaaggcg gcggaactgc ccggccggct
     7201 gttcgacatg atgatcgggg cgacaacgct cgtcatcgtc atcggctggt gctatgtcta
     7261 catgaaggct cacggccgct cgctgccatt gccgacctgg atcgagagca tccggatcaa
     7321 gctctatgtg ctgttcttga accgacttta tgtggatgaa ggtggccagc gcttggagca
     7381 ggcccgcctg tcgctcttcc aacggcttga gaagcacgcg gaggaacggg tgtgatgcat
     7441 gcctgcttga cgaacgtcaa gacaccgtat taatggtagc ccgccgcctt gggggggctc
     7501 tcagacgttc gtacgttccc ggggaagcga cgcatggacc gatgcagggg ggaatgaggt
     7561 gttcatgacg ttaacgctgt tggtccccct tctcctgctg gttgcctccg gtttggtgct
     7621 gatcggctcg gacagttccc gccatgcacg cgcgaagatt gccgcctatc ccattggtgt
     7681 cgcctttcta gccgccatgg cgacgctgtt tcaggtcacg acccagggcc ccctcgcctt
     7741 gcagttttac gatctctctt cggtagggag ctttgccatt cccatcgggt tttatatcga
     7801 tcggttgagc gcggtcatga tgactctgat caccggcgtc agcgtcatca tttacaccta
     7861 ctcgatcggg tacatgtatc aggacgccca tgcccgccgg tatctggcga tgatctgtct
     7921 gacagatttt gtgctgattt gtatggtcgc cagctccaat ctcatgatgc tttttctgtt
     7981 ctggcaggtt ctcagctacc tactctatgt gctggcgcac aatcatgctc atgctgggac
     8041 gctgaccgga gcgttcaaga cctttaccgt ccttcgcgtg gcggacacgg cgtttctcgc
     8101 cggaatcgcg ctcgcctatc agctctacgg cacgctcgaa ttccaggacc tgttccttcg
     8161 agcggccgaa acacaaatcg gcttgccgat cgggttcgga atggaggtca gcggggcaac
     8221 ggccgtgaca tttctgttct tcgtcggggc gatggggaag tcggcgcagt tcccgatcca
     8281 tttttggctg ccggggtcgc tcttcgcccc gacgcccgtg cacgcgctgc tgcacgccgg
     8341 catcatcaac gccggaggtt tcttaatcaa ccggctggct ccgctcttcg cccttagttc
     8401 gaccacgctg catgtggcct tcgtaattgg gacactgacc gccttccttg gcgcgacgat
     8461 gatgctggcc cagaacgata tcaaaaagac gctgggattc tccacgatcg gtcagatggg
     8521 ctatatgatc atggagtgcg gcctgggggc tttttcactg gccgtgtttc atctgatcgc
     8581 gcatgggttg ttcaaggcca ccgtgttctt gaattgtggg aatgtcattc acaaggccag
     8641 gcaggagccg cattttcctc atatcgacca tcagagagag gaagagggtt tgtctcgtct
     8701 gacttgggtc accggattcg tcaccaccct tttgatcccg cttttcatcc tgttgctcac
     8761 gcacggagtc ctccgcattc cgttgctcga atcgcaaggc acggtcatct tcctgttctt
     8821 catctggatt acgtcgtccc aggccatctt gacgttgacg agactccggc aggtcgcctc
     8881 ctggaaggtc tctgctgcca tgctgatgac cctgctgttt attgtcttcg tctatctatt
     8941 cgccgttgag tccttcacgg cgtttctgta tcccgatccg gacgcggtcg catcctactt
     9001 cagagccgcc gaccttcccg actggctctt cgacttcatc atcctcgccg ccacgattct
     9061 catgatcgcc agttggtctt acctgtacat gaccgcccac ggccgcaccg tccgaatgcc
     9121 aagctggatc gacgacatga gaatacgact ctacttgccc ttcatgaatc gcctgtatgc
     9181 ggacgaactc caccagctgc tgggggatac cctcatgcga ttggtccacc aattcgataa
     9241 acgagaacgg ggttggtcac gatgacggac ggcgctctcc tctcgtggct attggccggg
     9301 attcccttat tgggagcact cggcagcctt ctcttctggc cgaacgccga ccgcttgaaa
     9361 ttcttttcta tcctctggtc cgccgtgagc tgtgcatcaa tcatcggctt cgccggatac
     9421 ctcgcgatgc cgccggaagg gttcctgccg ctctatctgt tgccgctgac ggccgtgatt
     9481 tccctgctcg cacagccggc tcatgatcat tatcgtcaat cctgtctcat gaccttggtc
     9541 tgtctcggat tgggattgac cgtgctgacg agctctccgc cggtcggcaa tctcgcgttg
     9601 gcgttgctgc tggtcctggt cgccagtctg ctctaccgcc accacagcgc cctttggccg
     9661 atgtcatggt ggggcattgg gacctacggc ctcggtgtct tgtgcgccct gttggcggtc
     9721 gccgctggtc caccggtgtc ttccgttgca tcgctggtga cttgcgccgt tcttttgcct
     9781 ctggtgccat tccacgacgg ccatctcacg gcattgacac ggttgccggg caatttgccg
     9841 gcattcattg ttgtgctact tccgattgcg ggtctgcacg agcttccaca cgttctaccg
     9901 accgttccgg acgtcgtcgc ctggaccgtg agtttcttcg ccttactggg ggccctctac
     9961 ggcgcggtca aagcgctggc gcaatcccgc gtacggcttc tcctcgccta tggcagcctt
    10021 tcgttctttt cgatgctctg gtggttcgta gcggcgacgc gaaccgcgac gccacgagca
    10081 gccctcatgg ctggtacggt cggcctcgcg acgagcggac tgttggtcgc atggcaggtc
    10141 gtccggacca gatatggaga tgacatcgat ccacaggccg tcagcggcct ggcatccggc
    10201 atgccgcagt acgcggtgct gctctccttg ctggcgttgg cggccatggg cctgccgccc
    10261 ttcggcgtct ttgctgggtt tatggggctg ctgttgaacg cgccttttcc ctcgaccgtc
    10321 ggcctgttca tcacattgct cgcctggctc gctgcatcct ggtacatcat gcagatggtt
    10381 cagcgtctgc tgtacggcac tcgacggcag gagctgcgct acacggacgt gctcaagacg
    10441 gagctggcgt ccctcgtgat ggccgtcctc gtcctcctgg cactgggcct ggccccggca
    10501 acgctctttg cgcctggtca gaccggttct gagagagttt ctgtaaagga atcgtcgacg
    10561 tggaacaggt agaccgtacg atcgacatcc aaaccagacg gatggagctg cgcggcatcg
    10621 tgaagctcgc cggcgaagtc atcgctcagt attggccgat gcggacgttc gtgcaccaca
    10681 atccgctcca cagtctcgaa tatctcccgt tcgaggaaac ggtccgtcgc ggcaagcaat
    10741 tcatgggtgg gaatggctac cttccgggcc gtacgtaccg gcaatatctt cgttcgggtc
    10801 ggattcaact ccggcacctc gatgaagcgt tgagttcgcg cgtgcaaggc aaagaggtgg
    10861 tcgtgggttc ccgggttgtg acgcacggag aggtcctgcg cggctgtctt actgagggcc
    10921 tatgcgcagc cgttgtcgaa ccgctggaca atcagctgcc cgatgactcc ctcggcgtgg
    10981 tcgagcggct ggcgggacgg ctcgagtccg tcttgaccgt tccggatttg gcagagagga
    11041 tccgggggat cgtggacagc gacgaagacg ccttgggccg gtggctcacg ctctcgcact
    11101 ggtgcgatga cacgctcggc acccacatgg tggggcagat caacgaccag atgattaaat
    11161 ggtgcgaggc atttctcgac gagggtcatg caacatgggc catgcccgaa cgcgaaaagg
    11221 gcttctacaa agcctggaag gacattgcct ctcacgaatg gtcgccctgc ggcatcgcgg
    11281 acagcaaacg aaagatcgcg cagctcccag actacccaga ggatgcgctt ctcgaacatt
    11341 tggacgccct cggtattcct gcaaggctca gacaagatta tctttcgctg caactgacgg
    11401 cgctccctgg ctgggccggg ttcattaaat ggcgtgaaga ggaacgcgat tacccgtggc
    11461 agcaggccta tcccgttggg ctcgtcaaat acctcgccat tcgtctgtgg tacgcgcggg
    11521 aactcgtgca taaggcctgt agcgtccacc taggcatcga aggacgttac gatgcggtgg
    11581 tagcctacat gcgcagccac gccgaagaat attatttgcg ccgccagcgg gtcgccggtc
    11641 gtttgccggc cctctatgct gaggaggtgg accggctggc tcatcagaaa ggcggggggt
    11701 ggaagacggt gctcgatcgg taccaggccg aagtggtgcc gcgccaggaa gccgccacac
    11761 gccgcggggc ggcgcgaaaa cttttagcgc ttggccgttg cctggagatc gatccgaaac
    11821 agctcgtcga gaccgatccg gaaacgttac aacagctcgt cgcatggctc gaggccttcc
    11881 cggaatccga tcacggtccg gtttggctga aagcgttcga ggccggatat caggaacgcc
    11941 tgctcggaga gctgcgggcg aagagtcgcg aagccgatct cccgaacgcg cctcgtccgt
    12001 actcgcaatc agtctactgt atcgacgtgc gatcggagcc gtttcgtcgt catctcgaat
    12061 cgacggggcc caatgagacc tacggattcg ccggcttctt cgccgcattc attcgctatc
    12121 gagcctgggg caaagagcat gacaccgaac aatttccggt catcatgcgg gcgaagaatg
    12181 aagtgcgcga aattccccgg agctatctcg atcatcgagt gtcgaaacac aaggcccgcg
    12241 ccaagtgggt ccgcgccggc cacacgctgc tgcacgattt gaaagaaaac gtcgtgacac
    12301 cctacgtcat ggtcgaatcg ttgggatggt tctacagtct tcccatcttc ggaaaaacgt
    12361 tggtcccctc gctataccgg cgttggacgg cgtggttgag gcggctgttt gtgccatcga
    12421 ttgccaccac gctcaccgtc gataagctgg cgccgcaaga caccgccgag atgctgatcg
    12481 cagaccagca ggccctcgtc cgaaaagcgc tccaggaacg gctcggactg cgggcatccc
    12541 gcatcacccc ggcgctggta gagggcttgc gccagcgggc gctgaccggc tcaagcgagc
    12601 aggacgaggc ggtgatcgaa gcggcgaacc gtgccggtct ctcggcggat gccctgcaga
    12661 gctttgtcga gatcctgcgc cgcgaatatg acttgaacgc tcgatcggca tctcgccaaa
    12721 aagaacggat cacccgcacc ggattcacgc tcgaggagca ggcgctgacc gtcgatactg
    12781 ccctccgcat gatgggcttg accaaaacgt ttgcgcgtct cgtgctcttc tgctcgcatg
    12841 gcagcacttc ggataacaat ccgtacgaat cggcgctcga ctgcggcgct tgcggaggta
    12901 acgaaggcaa gccgaatgcc cgcgtcctcg cgatgatggc caataatcaa aaagtgcggg
    12961 aacggctgag caagggcggg atcgatatcc ccggtgacac gcatttcctg gccgggcaaa
    13021 tggacacgac gaccgatgag gtacagctgt tcgacctcga ggacgtgcct ccgacgcatc
    13081 gcgccgacct ggcccggctg caagaggatc tcagggaggc ctcggcgttg acaagcctgg
    13141 aacgctgcgc gcgctttccc gacgtgccgc ggtccctcga ggagaaacaa gccgaaaccc
    13201 acgtccgccg tcgcagtgcc gactggagcc aagttcgtcc ggagtggggc ctttccggca
    13261 acaccgcatt tctcatcggg cgtcgggaac taacgaaggg tttgaacttg gaaggccggg
    13321 tcttcctcca ttcgtacgac tatcgtgaag acccgacgaa ccgactgctg gaagtgctat
    13381 tgaccgctcc acaggtcgtc ggccaatgga tcaacatgga gcattatttt tctgcggtcg
    13441 acaatgacgt gtacggcagc gggagcaaga tttatcacaa tgtggtgggc cggctcggta
    13501 ttatgtccgg tccctggagc gaccttcgac tgggtctggc aagacaaacg gtcatgaacg
    13561 gctcaatgcc ataccatgaa cccatgcggc ttctcaccct gattgaagcg cctcgtgagc
    13621 ggatcgagaa gctgatctct cggcatgaag tgctccagaa ctattaccat aacgagtggg
    13681 tgcatctcgt cgccttagac cctgaggata atgtatggta tcgatatcac ccgaagggga
    13741 tatggcacgt cattccgtaa gatacctccg gtgcgagagg tggcttaaac tctctgagag
    13801 ggcgtgggaa aggagtctgt gaatgggcct tatattgcat ccaatgaagg aaattcgggt
    13861 gatcgtggcc ggtgagcagc gtgcctttgt gaccgatttg ctggatcgcg tcaatgccac
    13921 tgggtatacg attataggaa atgtgtccgg caagggccat cacggcgttc gcgaggcgca
    13981 tttcatgttc agtgaacagg aaagcctcga aatgattatg accgtggtac ctcaggagaa
    14041 agtcgagccg attctggcag gtctgcgtcc gctgtttgaa cggcattcag gagtgatgtt
    14101 cgttgccgac gtcgccgtga gtcgtcagga gtattttgcg aaaagcaaac cggcctgatc
    14161 attgtctccc ttcctctgcg ccacgccctt gtcttcacgg cagcaagttg tctacaatga
    14221 taccttgtcg ctggctgaag aggtactctc gtgaaggggc ttcgattcga acgcatcggt
    14281 cagggccgtt actatgatgt ggtgttccat ctcggcagca cctacgtgcc cgtgagtgat
    14341 gaaactattg aagagctcaa agcgcagagc ttacttcccg ccgaacggtt tctagatctt
    14401 cttatcgacc gtgtcggcta ctcgtcctac ctgaaagatc agatccgcaa agagttgaag
    14461 agttcgggcg atccggtgac gcagatcacc gtcctccaag gtgccatccg cgaactgtag
    14521 caggcgttga aaaacgccgt cggcttcgtt ctcgcatcac tccaaggctc aacgtaccag
    14581 acgacgtacg cttcgccttc tcgttcgctg cggccttgcc cccgccgctt ttgaccgccc
    14641 tgctatagac ggcgctaaca ctttcctgtg ccccttgcgg ttcgctcaca ttttgagccg
    14701 ccacatcatc accaggtttg cgccattgcc ataaccgcgt gaaggctttt cactaccgtt
    14761 cgcgcggacg aactatccga attgtgcctt catactggtc ggctgccacg actgacccat
    14821 cgagcacata ccgacatcgt gactctccct ctcttctagg tgtcccacgt gccgccttct
    14881 ttgtagagat tgttcatgat ctcgtttttt ttctgggggt cctgttcgta cttgatcgcc
    14941 ttcgtgtagt tctcaatcgc cagctgcac
//
LOCUS       DASTQY010000467         5742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1529514, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000467 DASTQY010000000
VERSION     DASTQY010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529514"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFR82_15010"
     CDS             complement(<1..299)
                     /locus_tag="VFR82_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HEU4502959.1"
                     /translation="MTGLEEMDIHALQQIVHTTRDARRKQTIAKFSPADRDQLIHKYH
                     PDYRASAYRPIRFGPNKGDRTVVELATLLEGDSPIQDDQDLTPHYTTDVLVIGGGG"
     gene            complement(377..1096)
                     /locus_tag="VFR82_15015"
     CDS             complement(377..1096)
                     /locus_tag="VFR82_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU4502960.1"
                     /translation="MATDTTDIQNIIDQPEVLKPRMVTIEIAGKRVEVPEGITVIKAM
                     WYAGQEVVRGVGCLGGFCGACATYYRTKDDPKVRTCLACQTAVQDGMSFTMMPPFPAR
                     KATYDIQTLQDPKQDLFNLYPETPLCRNCNACTEACPQQIDVREGVWKAVFGDFKSVS
                     EMFMDCVMCGMCAPVCIADIAPNLVALYVSRAQGAHFTEKPVGLDARIKEIQDGRFNE
                     EWNKILKMNDQQLAEHCATVK"
     gene            complement(1129..3357)
                     /locus_tag="VFR82_15020"
     CDS             complement(1129..3357)
                     /locus_tag="VFR82_15020"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEU4502961.1"
                     /translation="MAKADKIIYTKTDEAPMLATYSFLPIINAFSKAAGVTVELRDIS
                     LAGRVLAVFPDYLTPEQKQPDALAELGELAKTPEANIIKLPNISASAPQLVATIKELQ
                     SQGYKLPDYPENPKDDKEREIKARYDKVKGSAVNPVLREGNSDRRAPLSVKAHTRKHP
                     HKMGPWSSDSKTHVSHMTGGDFRSNEKSLTIPAATTAKIEFVGADGKVTVLKDKVALQ
                     AGEVLDATFMSVKALRTFIEEQIEDAKKKGVLFSLHMKATMMKISDPKIFGHAVTVFY
                     KDVFEKHGETLKKLGVDPDNGIGDLYAKIKSLPEDQRKAIEADIQEVYKKRPPMAMVN
                     SDKGITNLHVPSDVIIDASMPPVIRDSGKMWNPEGKLQDVKCVIPDSSYAPVYHEVVE
                     FCKKHGAFDPRTMGSVPNVGLMAQAAEEYGSHDKTFKAPGNGTIRIVDASGKTLLEHK
                     VEEGDIWRACQVKDAPIQDWVKLAVSRAKATGAPAVFWLNKNRAHDAELIKKVNAYLP
                     NYDTAGLEIKVMSPADACRFSLERMKEGKDTISCTGNVLRDYLTDLFPILEIGTSAKM
                     LSIVPLLNGGGLFETGAGGSAPKHVQQFQEEGYLRWDSLGEFLALAASLEHLAKVGNN
                     QVAKILADTLDQANAKFLESNKSPARKVGEIDNRGSHFYLALYWAQALAAQTADKKIA
                     ERFTKIAKDLGDNVAKIDQELLAAQGKPQDVGGYYHPNDEKASKAMRPSATLNRIIDA
                     IA"
     gene            complement(3384..5627)
                     /locus_tag="VFR82_15025"
     CDS             complement(3384..5627)
                     /locus_tag="VFR82_15025"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEU4502962.1"
                     /translation="MSMDLAKKLYAKMPDVCAKAKAQFGRGLTLAEKILVSHADNFDA
                     QTWERGKAMLALRPDRVAMQDATAQMAMLQFMQAGKKKAAVPSTIHCDHLIRAEMGSE
                     KDLLRAMDENKEVYNFLASAAKKYGIGFWRPGAGIIHQVVLENYAFPGCLIIGTDSHT
                     PNGGGLGGLAIGVGGADAGEVMAGLPWEVLHPKLIGVRLTGKLSGWASPKDVILYLCG
                     LLTVKGGTNKIVEYFGPGAETISATGKGTICNMGAELGATTSVFPFDQKMVAYMKITD
                     RSDLAELALANKALLVADPEVYQSPEKHYDQIVEVDLSTLEPHVVGPHTPDLARPISK
                     LAAEAQEKGYPVELKAALIGSCTNSSYEDISRSAHVAKQGLKAGLKAKTAFLVSPGSE
                     RIYHTMKRDGFLDTFEQLGGTVLSNSCGPCIGQWKRADGVKGKADSIVSSFNRNFPGR
                     NDGINETLSFLASPEVVTAYALTGDLRFDPVNQKLKSADGKEFKLEPPVGEELPAKGF
                     AKGEEGYVAPAENGEQLTVDIPPTSERLQLLQPFPRWDGKDLEKLPLLIKTKGKTTTD
                     HISPAGPWLKFRGHLDKISDNMFLGANSAFASEPGKGTNVLTGEANLTIAQIARAYKA
                     KGIGSIVVGDENYGEGSSREHAAMSPRFLNVRAVITKSFARIHETNLKKQGILPLTFA
                     DPKDYEKIEMNDRLSVMDLTNLAPGKPVAVIIHKASGDVKIQANHSMTAQQITWFKAG
                     SALNALN"
BASE COUNT         1073 a         1742 c         1571 g         1356 t
ORIGIN      
        1 ccgccgccgc cgatcactaa gacatcggtc gtatagtgcg gagtcaggtc ctggtcatcc
       61 tgaatcgggc tgtcgccctc gagcaacgtg gccagctcga ccaccgtccg gtctcctttg
      121 ttcggaccga atcgaatcgg gcggtaggcg ctagctcgat aatccggatg atacttgtga
      181 atcagctgat cgcggtcggc gggagaaaat tttgcaatgg tctgtttccg acgggcatcc
      241 ctcgtggtgt gaacaatttg ttgaagcgcg tggatatcca tctcttcaag accggtcatt
      301 ctcggctgtc gtcattaaaa cagagctccc ggccctcatt tgtcaccagc gaacggagac
      361 gttgaccgat gactcgtcac ttcaccgtcg cacagtgctc ggcaagttgc tggtcgttca
      421 tcttgagaat cttattccac tcctcattga accgaccgtc ttggatttcc ttaatccgcg
      481 cgtccaaacc caccggcttt tcggtgaaat gagcaccctg ggcgcggctc acgtagagag
      541 caaccaggtt cggagcgatg tcggcgatgc agaccggcgc acacatcccg cacatgacgc
      601 agtccataaa catttctgac acgctcttaa agtcgccgaa caccgccttc catacccctt
      661 cccgcacatc gatctgctgc gggcaggctt ccgtgcaggc gttgcaattc cggcagagcg
      721 gcgtctccgg gtagagattg aagagatctt gtttggggtc ctggagtgtt tgaatatcgt
      781 acgtggcctt gcgggcgggg aagggcggca tcatcgtgaa cgacatgccg tcctgcactg
      841 ccgtctgaca agcgagacag gtgcgcacct tcggatcgtc tttcgtccga taatacgtgg
      901 cacaggcccc gcagaagccg cccagacagc cgactccccg cacgacctcc tgaccggcat
      961 accacattgc cttaatgacg gtgatgccct ccggcacttc caccctcttc ccggcaatct
     1021 cgatcgtcac catgcgaggc ttcaacacct cgggttggtc gatgatgttt tggatatcgg
     1081 tggtgtctgt tgccatacgc tctccggcct ctcgccactc agcgagtgtt acgcaattgc
     1141 gtcgatgatc cggttcaacg tcgcactggg acgcatggcc ttggacgctt tctcatcgtt
     1201 cggatgatag tacccgccga catcctgagg cttgccttgc gccgccagca actcctgatc
     1261 gatctttgcc acattgtcgc ccaaatcttt ggcgatcttt gtaaacctct ctgcgatctt
     1321 tttatcggca gtctgtgcgg ccagggcttg cgcccagtaa agcgccaggt agaaatggct
     1381 gccccggttg tcgatctcac ctactttacg agcgggtgac ttgttgctct cgagaaactt
     1441 cgcgttggct tgatccagcg tatctgcgag gatcttggcc acctggttgt ttcccacttt
     1501 cgccagatgc tcgagcgagg ccgcgagcgc caggaactcg cccagcgaat cccatcgcag
     1561 atagccctcc tcctggaact gctgaacgtg cttcggcgcc gagccgcccg cgccggtttc
     1621 gaacaacccg ccgccattga gaagtggaac gatcgagagc atcttggcgc tcgtgccgat
     1681 ttccaaaatc gggaacaagt cggtgagata gtcacggagc acgttaccgg tgcaggaaat
     1741 cgtatccttg ccttccttca tccgctccag ggagaagcgg caggcatcag ccggagacat
     1801 gaccttgatt tcaagcccgg ccgtatcgta gttcggaaga taggcattca ctttcttgat
     1861 caactcggca tcgtgcgccc ggtttttgtt cagccagaat acggcaggag cgcccgtcgc
     1921 cttggcgcgg ctcaccgcga gcttgaccca gtcctggatc ggggcatcct ttacctggca
     1981 ggcgcgccag atgtcgcctt cttccacctt gtgctcgagc aaggtcttgc cggacgcatc
     2041 gacgatccgg atcgtgccgt tccccggagc cttgaaggtc ttgtcgtgcg agccgtactc
     2101 ttcggcggcc tgcgccatca agccgacgtt gggcacgctg cccatcgtgc gaggatcgaa
     2161 agcaccgtgc ttcttacaga actcgacgac ttcatggtac accggcgcgt agctcgaatc
     2221 gggaatcacg catttcacgt cctgcaactt cccctccgga ttccacatct tgccggagtc
     2281 cctgatgacc ggcggcatag acgcgtcgat gatgacgtcg ctcggcacgt gcagattcgt
     2341 gatgcccttg tctgagttca ccatggccat cggcggccgc ttcttgtaga cctcctggat
     2401 gtcggcctcg atcgccttgc gctgatcctc gggaaggctc ttgatcttgg catacaagtc
     2461 gccgatgccg ttgtcgggat cgacgcccag cttcttgagc gtctcgccat gcttttcaaa
     2521 cacgtcctta taaaaaacgg ttaccgcatg accgaagatc ttcggatcgg agatcttcat
     2581 catcgtagct ttcatatgca gagaaaacag gacgcctttc tttttggcat cctcgatctg
     2641 ctcctcgatg aaggtgcgca acgccttcac actcatgaac gtggcgtcga ggacttcacc
     2701 ggcctgcaag gcaactttgt ctttcagcac ggtgaccttg ccatctgctc ccacgaattc
     2761 gatcttcgcc gtggtggcag ccggaattgt cagggatttt tcgttcgagc ggaagtcacc
     2821 gccagtcatg tgcgagacgt gggtcttgga atcggagctc catggcccca tcttgtgcgg
     2881 atgtttgcgg gtatgggcct tcacggagag cggagcccgg cggtcggagt tgccttcacg
     2941 cagcacggga ttgacggcgc tacccttcac tttgtcgtaa cgagccttga tctcccgttc
     3001 cttgtcgtcc ttgggatttt ccgggtagtc cggcagctta tagccctggc tctgcaattc
     3061 cttgatcgtg gccaccaact gcggagccga ggcgctgatg ttcgggagct tgatgatgtt
     3121 ggcttccggc gtcttggcca gctcccccag ctctgccaga gcatccggct gcttttgctc
     3181 cggcgtcaaa taatctggaa agacggcaag gacgcggccg gcgagcgaaa tgtcgcgcaa
     3241 ttcaacggtc acgcccgccg ccttcgagaa agcgttgatg atgggaagga aggaataggt
     3301 ggccagcatc ggcgcttcgt cggttttcgt ataaatgatc ttgtctgctt ttgccatggt
     3361 gtgtccctct tttagtaact ggtttagttc agcgcgttca acgcagaacc ggccttgaac
     3421 caggtaattt gttgcgcggt catgctgtga ttcgcctgga tcttcacatc accgctcgcc
     3481 ttgtgaataa tgactgcgac cggcttcccc ggcgccaggt ttgtcagatc catgacgctc
     3541 agccggtcgt tcatctcgat cttctcgtag tctttgggat cggcgaatgt cagcgggaga
     3601 atgccctgct tcttcaaatt ggtctcgtgg atgcgcgcaa agcttttcgt aatgacggcg
     3661 cggacattca aaaagcgcgg tgacatggcc gcgtgctcgc ggctgctgcc ctcgccataa
     3721 ttctcgtctc caacgacgat cgagccgatg cccttggctt tgtacgcgcg ggcaatctgt
     3781 gcgatcgtga gattggcttc ccccgtcagg acgttcgtgc ccttgcctgg ttccgaagcg
     3841 aatgcgctgt tagcgcccag aaacatgtta tcgctgatct tgtccaaatg ccctctgaac
     3901 ttcagccagg ggccggccgg agaaatatga tcggtggtgg tcttgccctt cgtcttaatc
     3961 aacagcggca acttctcgag gtccttcccg tcccagcggg ggaacggctg cagcagttgc
     4021 aagcgctcgc tggtcggcgg aatgtcgacg gtgagctgct cgccattttc agcgggcgcc
     4081 acgtatcctt cttcgccttt ggcaaagcct ttcgccggta attcctcgcc aaccggcggc
     4141 tcgagcttga actccttgcc gtcagcactc ttgagctttt ggttgacggg gtcgaaacgc
     4201 aggtcccccg tgagggcata ggccgtcacg acttccggac ttgcaagaaa cgacagcgtc
     4261 tcgttgatac cgtcattgcg gccggggaaa ttgcggttga acgaactgac gatcgaatcg
     4321 gccttgcctt tcaccccatc ggcccgcttc cattgcccga tgcagggccc gcaggagttc
     4381 gacaacaccg tgccgccgag ctgttcgaag gtgtccaaaa acccgtcgcg cttcatcgtg
     4441 tgataaatcc gctctgatcc cggcgatacc aggaacgcgg tttttgcctt caggccggct
     4501 tttaaaccct gcttcgccac atgcgcggac cggctgatgt cttcatagga cgaattggta
     4561 cagctgccga tcaaggccgc tttcaactct accggatacc ccttctcctg agcctcggcc
     4621 gctagcttcg agatcggccg ggctagatca ggcgtatgtg gaccgacgac atgcggctca
     4681 agcgtggaga ggtccacttc cacgatctga tcgtaatgct tttctggcga ttgatagacc
     4741 tcgggatcgg caacaaggag agctttgttg gccagagcca gctcggccaa atccgatcga
     4801 tccgtgattt tcatgtacgc gaccatcttc tgatcgaacg ggaatacgga ggtggtcgct
     4861 cccagctcgg cgcccatgtt gcaaattgtg cccttccccg tagcgctgat ggtttctgct
     4921 cccggaccga aatattcgac gatcttgttc gtgcccccct tcacggtcag gaggccgcag
     4981 agatagagaa tcacgtcttt gggcgaggcc cacccgctca gtttcccagt cagccttacg
     5041 ccaatcaatt tcggatgaag cacttcccag ggcagaccag ccatcacctc accggcatcg
     5101 gctcccccga cgccaatggc caaccctccc agccctccgc cgttgggcgt atgggagtcg
     5161 gtcccaatga tcaagcagcc gggaaacgca taattttcca gcacgacttg gtggatgatg
     5221 cccgcacccg gcctccagaa cccgatgccg tacttcttcg ccgccgacgc aaggaagttg
     5281 tacacctcct tattctcgtc catcgcacgc aggagatctt tttccgaacc catttctgcc
     5341 cggatcaagt gatcgcagtg aatggtgctc gggacggcgg ccttcttctt gccagcctgc
     5401 atgaattgca gcatggccat ctgcgccgtc gcgtcctgca tcgcaacccg gtcgggacgg
     5461 agcgccagca tcgccttccc ccgctcccat gtttgggcat cgaagttatc ggcgtgagag
     5521 acaagaatct tctcggccag cgttaggccg cggccgaact gtgccttggc cttggcacac
     5581 acgtctggca ttttggcata gagttttttc gcaaggtcca tcgacatgat gacgtcctca
     5641 atgtggaatg tggaatttca aatatcgaac gctgaactcg ttcagtcgtt cacaagcgca
     5701 cgcctcacca cttatttcaa cggcggcact tctcttcgtt tg
//
LOCUS       DASTQY010000468         3652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1522858, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000468 DASTQY010000000
VERSION     DASTQY010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522858"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFR82_15030"
     CDS             <1..318
                     /locus_tag="VFR82_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502963.1"
                     /translation="HEKEDLMAQQERTQQWRGSDARGVVSQNLDSVESGVDVAIDSAK
                     DAVHEFRDKAEEVADAVLERVNRSWERQRPRIEAYMTSHPWIVFGGLILLAYLFSAKE
                     RTR"
     gene            complement(758..952)
                     /locus_tag="VFR82_15035"
     CDS             complement(758..952)
                     /locus_tag="VFR82_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502964.1"
                     /translation="MKYLSALLLLLVSGCTEHPYTVCRGEDFCTPALSHEEAIKASEI
                     KKTWNDEKLYVRPVRNREAT"
     gene            complement(1365..1766)
                     /locus_tag="VFR82_15040"
     CDS             complement(1365..1766)
                     /locus_tag="VFR82_15040"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4502965.1"
                     /translation="MKSPALLTMRKHKEQHMLPPSEDRRSSPRFEVEFRTLVSDKSGI
                     NDQVGVILDLSLSGCHVRVPILVYPTLVMELRLYAPDLDWPIFVEKAVVQWVKGDTFG
                     LHFLILQQAELIRLGRLIARITEDEAAQAQT"
     gene            complement(1909..2000)
                     /locus_tag="VFR82_15045"
     tRNA            complement(1909..2000)
                     /locus_tag="VFR82_15045"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            2389..2805
                     /locus_tag="VFR82_15050"
     CDS             2389..2805
                     /locus_tag="VFR82_15050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4502966.1"
                     /translation="MPTQDSEPLHSGTIQILLVDDHPHIRQLLRETIQSYDDLSIVGE
                     AVNGEEAVLLAAKLKPAAVVMDIHLPVLSGVPATTLIKINNPFTAVIGLTAGDPQEDE
                     KAMSIAGAALVLNKSEVLHGLYPAIVAAVKQVKNPV"
BASE COUNT          863 a          996 c          950 g          843 t
ORIGIN      
        1 catgaaaagg aggacctgat ggctcaacag gaacgaacgc aacaatggcg cggaagtgac
       61 gcccgaggtg tggttagtca aaatttggac agcgtcgaat caggagtaga tgtggcgatc
      121 gatagcgcca aagacgcggt acacgagttt cgcgataagg ctgaagaagt cgccgacgca
      181 gtattggagc gagtgaacag gtcctgggag cggcaacggc ctcggatcga agcctatatg
      241 acgtctcacc cctggatcgt gttcggagga ctgattctct tggcgtatct gttttcagcg
      301 aaagagcgca cacgttaaag cgccggctca ttgcggaatg gtgagggctc tcgcctcgta
      361 gtcgtggtcc acgagacggc ggttcaacga agcggtggct caaagttaag aacacaccat
      421 gtatgttacg aactgatgga attccgctct tgccctagcc gccgggtgtt cttacttgtt
      481 ggcggttgta acttctggtt gtgtcgatgg ttctgcactg cggcccggtg atattggttt
      541 gtgacgttca atccgacgaa tgattccgat ccgagatgta cttcctcgtc ccgatagtca
      601 ggctcggcgg ttgccatggc cctgctttct tccggccgcg actgaaggtt ctgtacgctc
      661 agcgaatcct ggacgtggcc gcattttaga caacgccatc tggccgtcca atccataaac
      721 cgatcctcaa caaggccatc tccgcaacgt gagcagatca tgttgcctcc ctgttgcgga
      781 cggggcggac atacagcttc tcgtcattcc atgttttctt aatctccgag gctttgatcg
      841 cttcttcgtg gctcagtgcc ggcgtgcaaa aatcctcgcc tcggcacact gtatatggat
      901 gctccgtgca gcctgagacc aatagcaata agagagctga aaggtatttc atggtaacct
      961 cccttgtctg gtggccaaag agcttcgcta gagtgaaaag gtaatcccag tctgcaagaa
     1021 ttcctatcct gctttgggat tgtagacttc cacgtagttg gaggcctacg tcaggccacg
     1081 caaaggcgcg cgggcaaacg gcttgccaaa ttttggcacg tgttctctct tcgtggggaa
     1141 tattggagac ttggcacgac tgaccagaaa acggtttgtg tccttaggga atgatacgcg
     1201 gtcttagccg ctactgacta gacgagcggc cttcctgaca ggggctcttc attcgtcaaa
     1261 cccactgatc tcttctgctg ccgcggaacg tataacctat aaggatgcag gctgagcaat
     1321 tgcagtttaa gaatctaaca gccgttggac gccctcctac cttcctacgt ctgagcttgc
     1381 gctgcttcgt cttctgtaat tctggcgatc agccgtccca ggcgaatcaa ttccgcctgt
     1441 tgcaatatca agaaatgcag accaaatgtg tcacccttga cccactgcac cacagccttc
     1501 tcgacgaaga tcggccaatc caaatcaggg gcatagaggc gtaactccat taccagcgtt
     1561 ggataaacca ggatcggtac ccgaacgtgg caccctgata gagacaagtc caggatcacc
     1621 cccacctggt cgtttatgcc gctcttgtca gagaccaacg tccgaaactc aacctcgaaa
     1681 cggggagatg atcgccgatc ttctgatgga gggagcatgt gctgttcctt gtgctttctc
     1741 atagtcagca aggcaggact cttcatggta atcctccagt tcttcttgct ggcaactgaa
     1801 acccctcttt aaacctatcc tgcagacgca cagctggcgc ccccacaacg gggtactacg
     1861 aaagggttag tcaaggatcg ctgagcggag ggtgctggat gtggaagttg gcggagaggg
     1921 taggattcga acctacggtc cggatgctcc ggacagcagc cataaggttg ctacgattac
     1981 agccgctctc gcacctctcc gatctcatac tattgtgcga ctggggaagt ccctactagg
     2041 atgaccccga gcttgcttca gttactcctc taaaccgccc tagaagagag tcactacatt
     2101 ccaaaccgtc tctctcaaag cgcacagtca taaccctctt gaacaagtta ggactctcgt
     2161 catttcgttc gtttattgct tgggggatat gagaacatga aatatcagca atccatgaca
     2221 tcaaccgaag acgcgcctgt ggtaggctga attatgcgag ggggcactac cggagcaaga
     2281 ggtctggatc aatcacatca cggctgctac gtagcccatc gtacttcctt cgagtcgttt
     2341 acgaacacca cgaaatggcg ttaaccagca cgctgtagga gcgactaggt gcccacccaa
     2401 gacagcgagc cgttacactc tggcactatc cagattctcc tcgttgacga ccatcctcac
     2461 attcggcagc ttttgagaga aacgatccag tcgtatgacg acctctcgat cgtgggcgag
     2521 gctgttaacg gtgaagaagc cgtgcttcta gcggccaaac taaagcctgc tgcggtggtc
     2581 atggatattc accttcccgt cttgagcggt gtaccggcga ccacattgat caaaataaac
     2641 aacccgttca ccgccgtcat tggcttgaca gcgggcgatc cacaggaaga cgaaaaggcc
     2701 atgtccatcg ctggtgcggc actagtccta aataaaagcg aagtgttgca cgggctgtat
     2761 ccggccatag tcgctgccgt caaacaagtc aagaaccccg tctagataca ccaagtcccg
     2821 gttcggtttt gtctgcttct acgctcttct cacaaaggtg cgtctcgcca tatgcaggat
     2881 ggactctgtc agcgatcgct ctcgcctcgc tccctagctc caccactgaa atgcatcatt
     2941 ccttgctccc gctcgcgagg tttcactgat agccgaccac acccttcacg agtaggattc
     3001 tacgtatgag gattgagatg gcatctcatc gcgaagacaa ccggttgata tgcacaagcg
     3061 tgtggtgccc gtcgaactac tgatctgcct cggttgtgat acttgattgt ttgccgcgtc
     3121 agtggccacg tgattccggg caaggtaaaa agtccgttgg ccatcagcca aatagagaaa
     3181 tgccgcaccg gaggttgctt gtgaatacgc ctctagataa cccagatccg cccgtgctgc
     3241 agatttgcac taagtgtcgc ctcgtgcatg acaatcgcag tggctggctc agtaagaaat
     3301 cctaccgaga agcgacgggg atcgatccca tcacctgccg tctgacgcat acctactgcc
     3361 ctctgtgtta cgactacttc gtgcagaaaa tccaagcagc atagaatccc ggaagagacc
     3421 aaagggggag ccttccccct cgcaactcac ccgccgacag cacttagagg ccagccaatc
     3481 acgctgatct ttggacgatt tgtatttctt agaagggatt tcagttgtga ggccactcag
     3541 cggcgggcga taagtcttgg cgattccaat cgtaacgacc agcagactga tcattcgagc
     3601 agaaagagag gcacaatctt cgcgcatcgg gaaaaggata taaaagatgg gc
//
LOCUS       DASTQY010000469         3190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1712162, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000469 DASTQY010000000
VERSION     DASTQY010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712162"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..768
                     /gene="rnhC"
                     /locus_tag="VFR82_15055"
     CDS             115..768
                     /gene="rnhC"
                     /locus_tag="VFR82_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU4502967.1"
                     /translation="MPSSSIERIGIDESGKGDYFGPLVIAAVFVDATTQEELTLMAVR
                     DSKKISDGRILELAPDIKTICPHSVIAIGPQKYNELYGKIRNLNRLLAWGHAKALETL
                     LERVSCERAISDQFGDERLILNALQEKGRRIVLEQRTKAESDLAVAAASIVARAEFLL
                     RLKRLSDEIGTVLPKGASPAVELAGRMIVKKHGQERLGTVAKLHFKTTKAVLEKTAT"
     gene            complement(781..1554)
                     /locus_tag="VFR82_15060"
     CDS             complement(781..1554)
                     /locus_tag="VFR82_15060"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEU4502968.1"
                     /translation="MNQNLSPLIELQKLDLRIMEITEQRRRIPERLHAVEAPLREAKQ
                     SLQDTSGAVEASVKERRSHEKDVEAQDAHTDKMRARLSELKTNKEYQAHLFELQMANK
                     KKSEIEDKVLACMEKIEQLQVAAKEAQEKVRIAEQAFTKEKQVLDEMDRKLSSELAEL
                     ESQQRERSAKVEPALLARYAKLKASRKDQPLAAIQRGMCAGCRLQIPPQLIAEVKRSQ
                     DLHTCPYCHRMLYWGGEAQADQKPLDHTKPTDLEVGESV"
     gene            complement(1614..1690)
                     /locus_tag="VFR82_15065"
     tRNA            complement(1614..1690)
                     /locus_tag="VFR82_15065"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1654..1656),aa:Ile,seq:cat)
     gene            complement(1838..>3190)
                     /gene="rpoD"
                     /locus_tag="VFR82_15070"
     CDS             complement(1838..>3190)
                     /gene="rpoD"
                     /locus_tag="VFR82_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU4502969.1"
                     /translation="LALRDQLKNGKIDVREIVPIQETEEDFEEEEVERDYEELRLKTL
                     EGLNTVRKVSTSLKGLYEKSRQAGKDPAKLKKIKKQIDAIREQVVDKMEAVNLHGVLK
                     DRMVQRVREMAIQIRTAEREIVSCQRRLGVGGEAGAELLRRLCRDRKDFQAVKRKTGV
                     SEETLQDVKKLYQAAKGRIRQLETEEALVSGEEIKDAVKHLDVAEEKVKRGKAELVEA
                     NLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAIT
                     RAIADQARTIRIPVHMIETINKLIRTSRHLVQKLGREPTPEEIAERMDLPLDKVRKIL
                     KIAREPISLETPIGEEEDSHLGDFIEDKKAISPLEAAIRYDLQRQINSALETLTPREE
                     KVLRKRFGIGEATDHTLEEVGQDFEVTRERIRQIEAKALRKLRHPSRSKKLRSFVESL
                     "
BASE COUNT          599 a          954 c          857 g          780 t
ORIGIN      
        1 tgaccgatgt agcggatccc ggcaggatgc tccccgcatc caagcagggg acgctacctg
       61 caactcgccc gcacgagcat cgactacctg ctcagcgtca cacagatcaa cagaatgccg
      121 tcctcttcga tcgaacgcat aggaatcgac gaatcgggaa agggcgatta cttcggcccg
      181 ctcgtcatcg ccgccgtgtt cgtcgatgcg acgacacaag aggagctcac gctcatggcg
      241 gtgcgagaca gcaagaaaat ctcagacgga cgcattctcg agctcgcgcc tgacattaag
      301 acgatctgtc ctcacagcgt catcgccatt ggcccccaga aatacaacga actctatggg
      361 aaaatccgaa acctcaatcg tttgctggct tgggggcacg cgaaggcgct tgagacgttg
      421 ttggaacgcg tctcctgcga gcgtgcgatc tcggatcaat tcggcgacga gcggctgatc
      481 ctcaacgcgc tccaagaaaa ggggcggcgc atcgtgcttg agcaacgcac gaaggcagag
      541 tcggatcttg ccgtcgccgc cgcctcgatc gtggctcggg ccgagttctt gttacggctc
      601 aagcgtctct cagatgaaat cggtactgtc ttacccaaag gggcatcgcc tgcagttgag
      661 ttggctggca ggatgatcgt gaagaagcac ggacaagagc ggttgggaac ggtcgcgaaa
      721 ctgcacttca agacgacgaa agccgttctc gagaagacag cgacgtagtg gtaaagagac
      781 ttacaccgat tctcccacct cgagatcagt cggctttgta tggtccagcg gcttttggtc
      841 tgcttgcgct tctccacccc agtacaacat gcggtggcaa taggggcatg tgtgcagatc
      901 ctgagagcgt ttcacttccg cgatcaattg aggagggatt tgcagccggc atccggcaca
      961 catgccgcgc tggattgccg caagcggctg gtcctttctc gaggctttta actttgcata
     1021 gcgggcgagc aacgccggtt cgactttcgc cgatcgctcc cgttgctggc tctccagttc
     1081 ggccagttcc gacgacagct tccgatccat ctcgtcgagc acctgctttt ccttagtgaa
     1141 ggcctgctcg gcgattcgaa ccttttcctg tgcttccttc gccgccactt ggagttgttc
     1201 gattttttcc atacaggcca acaccttgtc ctcaatttcg gacttctttt tgttggccat
     1261 ctgcaactcg aacagatggg cctgatattc cttgttggtc ttcagttcgg acaaccgcgc
     1321 ccgcattttg tcggtgtggg catcctgcgc ttcgacgtct ttttcatgag agcgccgctc
     1381 cttgaccgag gcttcgacgg caccactcgt gtcttgaagc gattgtttcg cctcgcgcag
     1441 gggagcttca accgcatgca gtcgttccgg gatccgccga cgctgttcag tgatctccat
     1501 gattcgaaga tcaagctttt gcagttcgat gaggggagac agattctggt tcaatgcact
     1561 cccaagtctg aggttcagga aggaaggata tgcaattccc gctgccggtc cactggtggg
     1621 cccactagga ctcgaaccta ggaccagctg attatgagtc agccgctcta accacctgag
     1681 ctatgggccc cctgggaaca gcttagctcc ggctcagggt gaggtcgaga ttcggatcag
     1741 actcgacagc cttccatcag ctcagccttg accgcagcct cgacgtcaaa ccgattttag
     1801 gcccgggtgg gagacagagt caaatcatcc gtcagtctca caagctctcc acgaaactcc
     1861 tcagcttctt gctgcggctc gggtgccgta gcttacgcaa agcttttgct tcaatctgac
     1921 ggatgcgctc gcgggtgact tcaaagtctt gcccgacctc ctccaacgta tgatcggtgg
     1981 cttctccgat gccgaatcgt ttgcgcaaga ccttctcttc ccgcggcgtc agggtttcca
     2041 gggcgctgtt gatttggcgt tgcaaatcat agcggatggc cgcctccagc ggagaaatgg
     2101 ccttcttgtc ctcgatgaag tcacccaggt ggctgtcttc ttcttcaccg atcggcgttt
     2161 ccaaggagat cggctcacgt gcgatcttca aaatcttccg caccttatcg agaggcaaat
     2221 ccatacgctc tgcgatctcc tccggggtag gttctcggcc gagcttttgc accaggtgac
     2281 gcgacgttct gatcagttta ttgatggttt caatcatgtg aacaggaata cggatcgtac
     2341 gggcctgatc ggcgatggcc cgcgtgatgg cctggcggat ccaccacgtg gcgtacgtgc
     2401 tgaatttgta tccgcgtttg tattcgaact tgtcgacggc cttcatgagc ccgatgttcc
     2461 cttcctggat caagtccagg aactgcagac cgcgattcgt atacttcttc gcgatgctga
     2521 cgacgagccg caggtttgcc tcaacgagtt ccgccttgcc tcgtttgacc ttttcctccg
     2581 ccacgtcgag atgcttgacc gcgtccttga tctcttcgcc ggaaaccagg gcctcctcgg
     2641 tctccaattg gcggatgcgt cccttggcag cctggtagag ttttttgacg tcctgaaggg
     2701 tttcttcgga cacgccggtt tttcgtttga ccgcctgaaa atctttccga tctcggcata
     2761 gtctcctcag cagttcggcc cccgcttcac cgccgacgcc gaggcgccgc tggcaactga
     2821 cgatctcgcg ctccgccgtc cggatctgga tcgccatctc acggacccgc tggaccattc
     2881 gatccttcag gaccccgtgc aggttcacgg cttccatttt gtcgacaacc tgttcacgga
     2941 tcgcgtcgat ctgctttttg atcttcttga gcttcgcggg atcttttccc gcctggcggg
     3001 acttttcgta cagccccttc aacgacgtcg acaccttgcg gacggtattc aggccttcca
     3061 gggtcttgag ccgcaattcc tcgtagtccc gctcaacttc ttcttcttcg aaatcctctt
     3121 ccgtttcttg tatcggcacg atctcgcgga cgtcgatctt gccgttctta agctgatcgc
     3181 ggagcgcgag
//
LOCUS       DASTQY010000470         6693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1120158, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000470 DASTQY010000000
VERSION     DASTQY010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120158"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..1378
                     /locus_tag="VFR82_15075"
     CDS             392..1378
                     /locus_tag="VFR82_15075"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU4502970.1"
                     /translation="MPRELSLAEIFQLGYYWETKILLTAVRLDLFSALDGKPKTPQEV
                     AARISAHEPTLTLLLNALVAMSLLIKTDDGYLNTTVSGKHLVRHSTHYIGHLLLLHDA
                     EWNNWAKLEQTIRSGKRSVDRHVFETDPELGSNVLAVLDRIGRQSGPEFAKQLHLNGP
                     VHLLDLGGGAGTNAIAFCQVYPELTAAVFDLPATLRLTEKTIKEAGLESRITLRAGDF
                     NRDGLGGPYDVVLMSDILHYQTFNANQALVTKVYDHLLPNGRLIIKDRFLDETGTGPA
                     WTTAFAVHILVNTQEGACYTTGEAMQWMTKAGFVSVSEVERTAVVHGMRSAR"
     gene            1386..2099
                     /locus_tag="VFR82_15080"
     CDS             1386..2099
                     /locus_tag="VFR82_15080"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEU4502971.1"
                     /translation="MDWLKHPGFFGTHATIGADVSQLMATFFTGLFVIGWIQARKRQS
                     DAHHWLMLGGMVAMLGFFVSYYLFRQLGVLAFEGKEGFGGSEALYHNVFIPLLTFHII
                     LVIIGLVMAVYMIVLGFRAQAIEQGRRILSGRILQTTWGKVGTIFGILCALVAVYLGY
                     LVAADRFRMGRLAVWVGLLALIAIVFAVEMAIQRIWPDGGQRHRTLGRFTMIIYCILF
                     VTGTTTYTMLYILYPGKIG"
     gene            2265..2726
                     /locus_tag="VFR82_15085"
     CDS             2265..2726
                     /locus_tag="VFR82_15085"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HEU4502972.1"
                     /translation="MHTEGIEERAYDDGAAQWFIRSECRGCGFKVGMDVPVGQHMFID
                     RLMWTDEALHRLERMPPYLAPVVREDVERDVRARGERVVTYDSVLRVRTGERIEWETE
                     AERRLENVPAPVRAMARIELERTAADRGMARITVALMEEVKAKYFGMGKTS"
     gene            2765..3808
                     /locus_tag="VFR82_15090"
     CDS             2765..3808
                     /locus_tag="VFR82_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502973.1"
                     /translation="MLHRMALRVLPSLTLAVTEPAVRKQKRLVMLVPGLVAFAVYRVA
                     KHLLPLAEPVVLLTVSGMVGLMTALLAYRVGRATAWVDIIGADGRWLLWWMAGWIGFA
                     YAVQLSLLVLALLWLVGYDYLQHPDGPAMMAMIISCTAVARDAFEIGHIRHVTLLGRP
                     CPTFPNGVALRSLLQSHALAIRPWVGIGLLAGALGASFGALVENDQAAALLQVAAITI
                     LGGAITLCAYFAGLRPSGTWMEGLMETKAAELLKYWWWPGLAFASTYYLVVAGVVIFL
                     LKQPGIPTSYAVCGGSLVAGVMALYGYYLGYRRQVEEQQGLSLSPALLRCPFVMGILT
                     KSSASVAAGVSKG"
     gene            3832..5673
                     /locus_tag="VFR82_15095"
     CDS             3832..5673
                     /locus_tag="VFR82_15095"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEU4502974.1"
                     /translation="MHAVLTIVALACGLALCNVLEFPALAEDAADLYYNVPGTPSGPA
                     APLPNETVYSRVGSYDSRLVVWFITQQHTYFGGFVLALPLFCALLEFLGLLTKKPALS
                     LRYDGLARDLAKVALLALSVTAVLGSLMLGMFLYFYPSFMRYMGGTFKAFMPAYAVVF
                     LGESLLLILYYYSWNRMAEPGLKWIHAAIGILTNIFGTALLLLANSWAAFMMSPAGVD
                     AQGRFLGNGWHLLHSALWNPLNVHRFLADIMSGGAVVLAYACYRFFTSKTDGERAYFD
                     WVGYVFLFVTVCALLPMPIAGYWLMRSVYAFRQSMGVTMMGGLLTWLFVVQALLIGAL
                     LLGVNYYIWQSMARIKGGERYQTYYQFLVGALTLALFIWLTPHTVLMSAGEVKAMGGA
                     QHPVVGNYGVMSAKNGAVNIMILITALSYLYYRRANRTMTVSWVTRGNLVIGTLFAVG
                     LLNVTWLSVYGFYLPAKVRVGLSTPQAVTTLTVIVVSLLINRAMLRGSIVHGPVQWGK
                     ISIRGMVVLFGLAAAFTWVMGLMGYIRSSGRLSWHVNELMADVSPWAFTPELGFAAKM
                     VTLNMVVFWSAVLALFWMCQRGQQQELKEEWFEESSRTLASVPPAET"
     gene            5699..6346
                     /locus_tag="VFR82_15100"
     CDS             5699..6346
                     /locus_tag="VFR82_15100"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEU4502975.1"
                     /translation="MKRTSNISAVLVAIALLVPASGSGSAQGQRLVLEDFQTKEADGF
                     PASWDHESQRSHSKGREAYKIQAENGSNYLSAKDAGQRIKKKKIEWDPKAYPVLTWRW
                     RLNKAAEGTEPLAAIYASLDTDLMFIPVFTKYIWSATKPEGTLIEGGMFSGSEIVVQS
                     GTTPVGQWVEEKVNVYEDFKRIHQHEPAAKAWGISIVAGSGVEIDFGPMVAMSDK"
     gene            6367..>6693
                     /locus_tag="VFR82_15105"
     CDS             6367..>6693
                     /locus_tag="VFR82_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502976.1"
                     /translation="MSPVHRWFDIVFGLVVMGTVGALIGLIMGGGLMPISIVVGLAMG
                     GVIGFLGGRRFLISILIGSVLGGALAWLVAGTERISVGAGTGAAMGGFLGVQISMLLD
                     VRAARKA"
BASE COUNT         1302 a         1738 c         2086 g         1567 t
ORIGIN      
        1 agttgaagtc gcggacggtg gaacggccaa tctgaaggcc gaactcaagt aagccacgtc
       61 tgattttttt gcggggcaac tcgcaatcca aaaggcagcg gagtgatccg ctgccttttg
      121 ttttttcact cccgacagca gggcatcgcg ccttgcgttt ccgttttcaa actaggtaag
      181 atgccgtctt tcaaggagta ttttggctgc acggtgttca taaaagccga cagttccacc
      241 tgcccgctcc gacgcgccga gacgcgtcct cccgcaagcg cgacgcaggg agtgaagggc
      301 aaggcgtact cttacccgta tgctgaggcc ctgagcgacg cgagaacaat gccggacgac
      361 ctttccaaca tactgctgta gggggatggg tgtgccacgg gaactgagtc tcgctgaaat
      421 ctttcaactt ggttactact gggagaccaa gattctgctg acggctgtcc ggctcgacct
      481 tttctctgca ctcgatggca agccaaaaac cccccaggag gtggccgcca gaatcagcgc
      541 acacgagccg acgctcactc ttcttttgaa cgctctcgtg gcgatgtcct tattgataaa
      601 gacagacgac ggctacctca acaccacagt gtcaggaaag cacctggttc ggcactcaac
      661 tcattacatc gggcacctgc tattgttgca cgatgcggaa tggaacaatt gggcaaaatt
      721 ggagcagacg atccgttcag gaaagcgatc ggtcgaccgg catgtcttcg aaacggatcc
      781 ggagttgggc agcaatgtct tggcggtgct tgatcgcatc ggtcgccaga gcggtccgga
      841 atttgcgaaa cagttgcacc tcaatgggcc cgttcatctg ctcgatctcg gaggcggagc
      901 agggaccaac gccatcgctt tttgtcaggt gtatcccgaa ctgaccgccg ccgtgttcga
      961 tcttcccgcg accctgcggc tcaccgagaa aaccatcaag gaggccggtc ttgaatctcg
     1021 aataacgttg cgagcagggg atttcaaccg cgatgggctc ggagggccct acgatgtcgt
     1081 gctgatgtcc gacatcctgc attaccagac gtttaatgcg aaccaggcct tggtcacaaa
     1141 ggtttatgac catctgttgc ccaatggccg attgatcatc aaagaccggt ttttggacga
     1201 aacgggaacg ggccccgcct ggaccacagc attcgcggtg cacatcctcg tgaatactca
     1261 agaaggggcc tgttacacaa caggggaggc gatgcagtgg atgaccaaag cgggattcgt
     1321 ttcagtcagt gaagtagaac ggacggcggt ggtccacgga atgagatctg cgaggtaacc
     1381 gctcaatgga ttggttaaag catccgggat ttttcgggac acacgccacg atcggcgccg
     1441 atgtcagtca gctcatggcg acgtttttta ccggtctctt tgtgatcggc tggattcagg
     1501 cacggaagcg ccaatcggac gctcaccact ggctgatgtt ggggggcatg gtggcgatgc
     1561 tgggcttctt cgtgagttat tatcttttca ggcaactcgg cgtgttggcc tttgaaggaa
     1621 aagaaggatt tggtggatcc gaagccctct accacaatgt gtttatcccg ctcctcacct
     1681 tccatatcat tctcgtgatt attggattgg tcatggcggt gtacatgatc gtgctggggt
     1741 tccgtgccca ggcgatcgag caaggaagaa ggattctaag cggccgtata cttcaaacga
     1801 cgtggggaaa agtcgggacg atcttcggga tactctgtgc cctggtcgca gtgtacttgg
     1861 gatacctggt ggcagcggat cgtttccgaa tgggacgact tgccgtgtgg gtgggacttc
     1921 tggcgttgat tgcgattgta tttgcggtgg aaatggcgat ccagcgtatt tggccggatg
     1981 gcggacagcg ccatcgaaca ctcggacgct tcacgatgat catctattgc atcctatttg
     2041 tcaccggaac gaccacgtac accatgttgt atatcctgta ccctggcaag atcggttaac
     2101 agagtgttga aaggtgctct tcatcaaacg cgcccatccc gcgcggctct gttgcatccg
     2161 gccgacccgc cgatcgcttc gcgatcgatt tccggggacg cgcgtttcac tgagctttgc
     2221 tctcgcatcg ggaagccatg ttgacgtgcg ggtgcggtcg ctggatgcac acggaaggga
     2281 tcgaagagcg ggcctatgac gatggcgcgg cgcaatggtt catccgttcg gaatgccggg
     2341 ggtgtggctt taaggtagga atggatgtgc ctgtcggcca gcacatgttc atcgatcggc
     2401 ttatgtggac ggacgaggca ctgcatcggt tggagcgaat gcctccctat ctggcgccag
     2461 ttgttcgaga ggacgtcgag cgcgatgtca gggcgcgcgg tgaacgggtc gtgacctacg
     2521 actcagtgct gcgcgttcgg accggcgagc gaatcgagtg ggaaactgag gccgagcgcc
     2581 ggttagagaa cgtccctgct ccagtgaggg ccatggccag gatcgaactg gaacggaccg
     2641 cagcagatcg ggggatggct cggatcactg tcgcgctgat ggaggaagtg aaggcgaaat
     2701 acttcgggat gggaaaaacg agctgagtaa cggacaaaga gctgccagaa gagcaaggga
     2761 agaaatgttg catcgaatgg cgttgcgagt gttaccgagt ctgactctgg cggtcacgga
     2821 accagccgtg cgcaagcaga agcggttggt catgttggtg ccaggtctgg ttgcgtttgc
     2881 ggtatatcga gtggcaaagc acctcctgcc gcttgcggaa ccggtcgtgc tccttacggt
     2941 cagcgggatg gtgggactaa tgactgcctt attggcctat cgtgtcggtc gggcaacggc
     3001 gtgggtcgac atcattggag cggatggtcg ttggcttctc tggtggatgg ccggttggat
     3061 cgggtttgcc tacgccgtcc agttgtcgct tcttgtgctc gccctgctct ggctcgtggg
     3121 ctatgactat ctccagcacc ccgacggtcc cgccatgatg gcgatgatca tttcctgtac
     3181 cgcggtggcg cgggatgctt tcgaaatagg ccacatacgt catgtgacat tattgggacg
     3241 gccatgtccc acctttccga acggtgtggc attacgcagt ctgctccagt cccatgcgtt
     3301 ggccatccgt ccgtgggtgg gcatcggact gctggccggt gcactcggtg cctcattcgg
     3361 tgcgttggtc gagaatgacc aggcagcggc ccttctccag gtagccgcga tcacgatctt
     3421 gggaggcgcc attacgctgt gcgcctattt cgccggactg agaccgtcag gcacatggat
     3481 ggagggattg atggaaacca aggcggctga attgctgaag tattggtggt ggcctgggtt
     3541 ggcgtttgct tcgacctact atctggtcgt tgctggtgta gtgatttttc tcctgaagca
     3601 gcccggcatc cccacctcct atgccgtctg cggtggcagc ttggtggccg gcgtgatggc
     3661 gctctatggg tactacctgg gctatcgccg gcaggtggaa gagcaacaag gactcagcct
     3721 gagccccgct ttgcttcgct gtcctttcgt gatgggcatc ctcacaaagt cgtcggcctc
     3781 cgttgctgcc ggtgtgtcaa aaggttgaat atgctatcga ggtttagatc cttgcatgcg
     3841 gttctcacca ttgtggcgct tgcctgtgga ctcgcactct gtaacgtgct cgagtttcct
     3901 gccttggcgg aagatgccgc ggatctctat tacaacgtac cgggtacacc atcaggacct
     3961 gccgctccct tgcccaacga aacggtttac tctcgtgtcg gatcatatga cagccgctta
     4021 gtagtgtggt ttatcacgca gcagcacacc tacttcggcg gcttcgtgct cgctctcccg
     4081 ctgttttgcg cgctgctcga atttttgggc ttgctgacaa aaaaacctgc actgtcactt
     4141 cgctacgacg gtttggcgag agatctggca aaagtcgcgc tgctcgccct ttcggtgacg
     4201 gcagttctgg gcagcctgat gctcggcatg ttcctgtatt tctatccgag tttcatgcgc
     4261 tacatgggtg ggacgttcaa ggctttcatg ccggcctatg ccgtcgtctt tctcggcgag
     4321 tcgctgctgc tcatcctcta ctactacagc tggaaccgca tggcggagcc tggcttgaag
     4381 tggatccatg cggcgatcgg catcctgacc aacatttttg gtacggcgct gctgctattg
     4441 gcaaattcct gggcggcctt catgatgtcg cctgccggcg tggatgcgca gggacggttc
     4501 ctcggcaacg gctggcattt gttgcattcc gctctttgga atccgctaaa cgtccatcgt
     4561 ttccttgccg acatcatgtc gggcggcgcg gtcgtgctgg cctatgcctg ctatcgcttt
     4621 tttacgagca agaccgacgg ggagcgcgcc tactttgact gggtaggcta cgtctttctc
     4681 tttgtgaccg tgtgtgcgct tctgccgatg ccgatcgcgg gctattggct gatgcgctcg
     4741 gtgtatgcgt tccgccaaag catgggcgtc acgatgatgg gcggtctgtt gacatggctc
     4801 ttcgtcgtcc aggccctgct gatcggcgcg ctgctccttg gcgtcaatta ttacatctgg
     4861 cagagcatgg cacggatcaa gggaggcgaa cgttaccaaa cctattacca gttcctggtt
     4921 ggagcattga ccctggcgct gtttatttgg ttgacgccgc atacggtgct gatgtccgcg
     4981 ggtgaagtga aagcgatggg cggcgcgcaa catccggtgg tgggaaacta tggcgtgatg
     5041 tcggcaaaga acggcgcggt caacatcatg attctcatca cggcgctcag ttatctttac
     5101 tatcggcggg cgaatcgaac gatgaccgtt tcttgggtca cgaggggaaa tctcgtcatc
     5161 gggacgctct ttgcagtcgg tcttttgaat gtgacatggc tctcggtgta cgggttttac
     5221 ctgcccgcaa aagtgcgtgt gggcctttcg acgccgcagg ccgtcacgac gctcaccgtc
     5281 attgtcgtca gcttgctcat caaccgcgcc atgctcagag gatcgatcgt gcatggtccg
     5341 gtgcaatggg ggaaaatctc gattcgcggc atggtggtgc tgttcggtct ggcagcggcc
     5401 tttacgtggg tgatgggtct gatggggtac attcgctcct cggggcgctt gtcctggcac
     5461 gtgaatgagt tgatggccga tgtctcaccg tgggccttta cgcccgaact cggcttcgcc
     5521 gcgaagatgg tgacactcaa catggtcgtg ttttggagcg ccgtcctcgc gttgttctgg
     5581 atgtgtcagc gggggcagca gcaggaattg aaagaagaat ggttcgagga gtcgtcgcga
     5641 acattggcat cggttcctcc agcggaaacc tgagggatta gaaagggtca tggcgggcat
     5701 gaagaggacc agcaacatca gcgcagtttt ggtggcgatt gccctgctcg ttcccgcatc
     5761 aggatcggga tcggctcaag gacagcgtct cgtcctggag gatttccaaa ccaaggaggc
     5821 ggacgggttt ccggccagct gggatcatga aagccagcgc agccattcca agggtcggga
     5881 agcgtataag attcaagccg agaacggctc caattacctg tcggcgaaag atgcaggaca
     5941 acgaatcaag aaaaaaaaga tcgaatggga tcccaaggcc tatccggtgc tcacctggcg
     6001 ctggcggctg aacaaggcgg cggaggggac cgaaccactc gcggccatct atgcgtcgct
     6061 cgatacagac ttgatgttca tcccggtttt caccaaatat atttggagcg cgacgaaacc
     6121 ggaggggacc ttgattgaag gcggaatgtt cagcggctcc gaaattgtgg tccagagcgg
     6181 gaccaccccg gtgggacaat gggttgaaga aaaagtaaac gtgtacgaag atttcaagag
     6241 gatccatcag catgagcctg ccgcaaaagc ctggggcatc tcaattgtgg ccggttcagg
     6301 cgtcgaaata gattttgggc cgatggtcgc gatgtcagat aaataggcat ggatgccatg
     6361 gttgccatga gccctgtgca ccgctggttc gatattgtct tcggcctcgt cgtgatgggc
     6421 acggtgggag ccttgatcgg gctcatcatg gggggcggac tcatgccaat ctcgattgtg
     6481 gtcggactcg ccatgggcgg agtcatcggg ttcctgggcg ggcggcgctt tcttatcagt
     6541 attctgatcg gtagcgtgtt gggcggtgca ttggcgtggc tggtggcagg aacggaacgg
     6601 atctcagtcg gtgcagggac cggagcggcc atgggcggat ttctgggcgt gcagatctcg
     6661 atgctcttgg atgtgcgtgc cgcgagaaag gcc
//
LOCUS       DASTQY010000471         2640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1488225, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000471 DASTQY010000000
VERSION     DASTQY010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488225"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFR82_15110"
     CDS             complement(<1..576)
                     /locus_tag="VFR82_15110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4502977.1"
                     /translation="MREQQALVVDDHPIIRDGIKQLLARAFPALHIRDSSGSDRVLEE
                     ICSFPWAFVTLDINLPGQNGLHIIREIQVCCPKVPIIVFSLFSEDQYGPRALRAGAAA
                     YLSKERSPLELVEVVRSVLRGEKKGQSPSAAPVLSSRETEVLTLLGKGMNRQQIAHEL
                     GINEKTVSTYRARLLHKLNVRTTVNLLRFAVD"
     gene            complement(1281..>2640)
                     /locus_tag="VFR82_15115"
     CDS             complement(1281..>2640)
                     /locus_tag="VFR82_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502978.1"
                     /translation="GPRLQPGDYIHLNGRVSKFSEGAWGIMRVLDKEVPDLQKLPAGY
                     SRRNEIPKPLGVCPADAPVKAFNVVAMDYPGMKLNPNAPDVIEVDFERTIKMVNPEAK
                     IYTLEEEASKVASGIQPMPLTLRANVGDCIKVKLTNRMKEGRASFSAISLAFDPKDSL
                     GANVGNNPGDQTVGPGENRTYTYYADPFIGETASLVWDWGNIVVNPRNGLYGALVIGP
                     KGAKYRDPKTGADISMKNSWVADVIVDRTIQGYEHRTNYRDVALFFQDEDNIIGTSFM
                     PYVQNTAGLTAVNYRAEPYKFREEKGCTLGKVFQPCVVDKPEDPATPIIEAHAGDPVR
                     IHVFGASNEQNGMFSVERHEWPIEPFMRGADMISVVEFSGSETLDAFIPTAGGPFRMP
                     GDYVWSNQRLPYAQSGQWGYLRVLPPGDNRIKGLTDARSGSQRAGVQEEGRGDVLPVS
                     VK"
BASE COUNT          570 a          748 c          716 g          606 t
ORIGIN      
        1 gtctaccgca aaccgcagga gattgacagt cgtacgaacg ttaagcttgt gcagcagtct
       61 ggccctgtat gtgctcacgg ttttctcatt gattccaagt tcgtgagcga tttgctgtcg
      121 attcatccct ttgccgagga gtgtaaggac ctcggtttcg cggctggaca acactggagc
      181 agcagacgga ctctgacctt ttttttctcc ccgcagaacg gaacgaacga cctctactaa
      241 ctccaacggt gagcgctcct ttgacaggta tgcggcggct cctgcccgta aggcgcgcgg
      301 accgtattgg tcttcggaaa ataggctgaa gacaataatg gggaccttcg ggcaacagac
      361 ttgtatctct ctgatgatat gaagaccatt ctgaccaggg agattgatat ccagcgtgac
      421 aaatgcccag gggaagctac agatctcttc caggacccga tcactccctg aagaatcgcg
      481 aatgtgcagc gctggaaatg ctcgcgcaag caactgctta atgccatctc gaatgatcgg
      541 atgatcatcc acgacgaggg cttgttgctc acgcatgtcg gtgtgctcgt tttttggagg
      601 caagccgcga cgaggccaac gcgaattgaa cgaccaagag acagttctcg aaggacagat
      661 actccccctg ggtcaaggaa ggtcctgttt caccattcgg gttacctgga cactacactg
      721 gccacacgtt gctacgatcc tgtgattttg catgtatggt ggatgacacc caaatggctc
      781 gactatccgc caaaatgtgc gcgttgtcta tcagcgatga cctacagatc actttttctc
      841 aaacgggaca gtggttgtca ggaggaccac ggaagagagg taacgtacgg tgccggttac
      901 tgctggatgc tggggttctt aaatgcggtt aagccgctaa cggctttggc tcttcagtat
      961 gccacctgcg gccacagttg aagcagacgt actgaagaat agtggttcct gttacgaggt
     1021 caatccctgt ctctgcccga aggctgccct ggcatttttg gcaccgttca aagtgcttgg
     1081 cgttttcctg agccataagg gttagagaat agtgatcctg acgccggttt gtctatcagt
     1141 ttcagcctac acccttgtgc gattgcgtga caatctaggg attccactgg gcgggagaca
     1201 aggacggtga gctcgagtca cgggaggatt gtgctgggcg agggaagtga tagtgcgacc
     1261 tccctcgccc tcatgaaagc ttatttcacc gagactggaa ggacatctcc acgaccctcc
     1321 tcttgaacgc cggctcgctg gctgccggag cgggcatctg tcaacccctt gatgcggttg
     1381 tcgcccggag gcaaaacccg cagatagccc cactgccccg actgggcata gggcaggcgc
     1441 tggttcgacc acacatagtc gcctggcatg cggaatggcc cgccagcagt gggaatgaac
     1501 gcatccaagg tttcggaccc cgagaactcc accacgctga tcatgtcggc ccctcgcatg
     1561 aacggctcga tcggccattc gtggcgttcg acgctgaaca tcccgttttg ctcgttgctc
     1621 gcaccgaata cgtggatgcg gaccggatca ccggcatgcg cctcaataat gggcgtggcc
     1681 ggatcttcgg gtttatccac gacgcaaggt tggaacacct tgccgagagt acaacccttt
     1741 tcctcccgga acttgtacgg ttccgcccga taattcaccg ctgtaagacc ggcggtattt
     1801 tgcacatagg gcatgaaact cgtcccaatg atgttgtcct cgtcctggaa gaacaaagcg
     1861 acgtcacggt agttggttcg atgctcgtat ccctgaattg tacgatccac gatgacgtcc
     1921 gccacccatg aattcttcat ggaaatgtcg gcgccagtct tcgggtcacg gtatttggca
     1981 cccttggggc caatgaccag cgctccatag aggccgttcc tcggattcac cacaatatta
     2041 ccccaatccc agacgaggga ggctgtctcg ccaatgaacg ggtcggcgta ataggtgtag
     2101 gtcctattct cccccgggcc aacagtttga tcgccaggat tattgccgac attcgccccc
     2161 aaagaatcct tggggtcaaa ggccaggctg atggcagaga acgaagcccg gccctctttc
     2221 atgcggttcg tcagcttgac cttgatgcaa tcaccgacgt ttgcacgcaa cgtgagcggc
     2281 atcggttgaa ttccgcttgc caccttggag gcctcttcct ctaaggtgta gatcttcgcc
     2341 tccgggttca ccatcttgat ggttcgttcg aagtccactt caatgacatc cggcgcgttt
     2401 ggattcaatt tcatccccgg ataatccatc gcgaccacgt tgaaggcctt caccggagca
     2461 tcggcggggc agaccccaag gggtttcgga atttcatttc gacggctata gcctgccggc
     2521 aacttctgaa ggtccggcac ctccttgtcc aacacacgca tgatgcccca agcaccctcc
     2581 gagaacttcg acacccgacc gttcaaatgg atgtagtcgc cgggctgcag ccgcgggccc
//
LOCUS       DASTQY010000472        11912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1537762, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000472 DASTQY010000000
VERSION     DASTQY010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 11912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11912
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537762"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VFR82_15120"
     CDS             complement(<1..858)
                     /locus_tag="VFR82_15120"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU4502979.1"
                     /translation="MDVLSEVLKAVKLDGAIFYTAECSAPWGFRSPPSRVMASYLSPQ
                     PKHVIVYHLVTEGRGYAHIEDDTRPVPLEAGEIVVFPHGHPHILGNGSPIVPVDSAKE
                     LQRILSEDLKIYRHGGGGELTRIICGYLACEPQLSRILLSGLPPILKVSIRNDASGEW
                     LENSLRYSVDNSDASRPGGETVLAKLSEVLFIETLRRYIEQLPQKQAGWLAGVRDPEI
                     GKALAFLHRQPARPWTIATLAAEVGMSRSVLAERFRQYLSETPMAYLARWRLQLGAQM
                     LHATSRSVAE"
     gene            1051..1872
                     /locus_tag="VFR82_15125"
     CDS             1051..1872
                     /locus_tag="VFR82_15125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4502980.1"
                     /translation="MATISLTPDVETLKTRLRTMWMAGDYDRFSRYMEASAREFYDRI
                     PLFPGCTFLDVGCGSGQLALMAARDGLHVTGVDIAANLVERARSRAQAEDLQARFEEA
                     DAEALPFEDESFDVVASLIGAMFAPQPEVVAGELMRVCKPGGTIAMANWTPQGFVGQM
                     FKAVSKFIAPSGMPSPVLWGDEATVRERLGHGLTELRLTRRQYAFMYDFSPAAVVELF
                     KLYYGPINQAFGALDAAGRDSLRGELEGLWSAHNRAGIDRTTVYAEYLEVIGIRA"
     gene            2209..2658
                     /locus_tag="VFR82_15130"
     CDS             2209..2658
                     /locus_tag="VFR82_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502981.1"
                     /translation="MRILTFGAFLIAGACLVAADYTLAQAGSGGSGGASGSGMTSQGL
                     ASEPAGIGERGTGQTQSTLERSGVPAQKGMSSEPAGIGEKGTGQTQSTMERSGLGQNN
                     AQSQSPLPSGIGEQGTGQTQSTMERAGGGQGATQGQSSQSAMDRPER"
     gene            complement(3315..3791)
                     /locus_tag="VFR82_15135"
     CDS             complement(3315..3791)
                     /locus_tag="VFR82_15135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4502982.1"
                     /translation="MLVSVEGNHGVQNDLASRSQLVSTSSTDARPVQYRVLLVDDDPG
                     TRQLLEDILEKYQDIRIVGQAGDGQQAVAMAIEYKPDVILMDIGLPYLDGVEATQCIR
                     KSCPETVVICLTGHFSPPRYSAMRTAGAAAFVCKNQVLAIHEMILSTLGQWTRWKY"
     gene            complement(4308..4700)
                     /locus_tag="VFR82_15140"
     CDS             complement(4308..4700)
                     /locus_tag="VFR82_15140"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4502983.1"
                     /translation="MRRRYDHIDLRVRSLPEARVFYEALLPALGFTRRIEVDGWLQFE
                     AVGGAGGSTEFFGVTEAPGHRANECRVAFWAESTAEVDRLASIVVQTGGRQIEGPAYL
                     EGPGYYALFFEDPSGNRLEICHRVAGAI"
     gene            4839..5747
                     /locus_tag="VFR82_15145"
     CDS             4839..5747
                     /locus_tag="VFR82_15145"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU4502984.1"
                     /translation="MFRLIQRRASSMPMRVLGKTGVNLPILGFGTAHSGLRLNTREAV
                     HLYERAFGEGITYFDTAPEFAGYGKAQMQLSHFLKQVRPQVFLVTKCYEPDGERALQL
                     LQKSLKELGTSYADLVFVHSVGADKMDPRTVFGNKGTYAALMKAKALGLTRYVGLSGH
                     SRPGRFAQAIRQFDVDVLLNAVNFVDQHTYNFEAEVWPAAARANIGLIAMKVYGGAQD
                     PPPRPDRLSHSMMAAEHHDLAFRYALSLPQVCCAVIGMATTRELEQNVARARAFKPLS
                     GGEVRSLRDAGRLMARQWNDHFGPVT"
     gene            complement(5862..6560)
                     /locus_tag="VFR82_15150"
     CDS             complement(5862..6560)
                     /locus_tag="VFR82_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502985.1"
                     /translation="MHRRFNASLTKEPKGLSHRGSAFLYVLILATAVAGCSKAEFVPA
                     ITLAPARPIIDATAEFHDMYPAPALVSGKENFGLTGPDAEAVPPRVLANQVQHELLDA
                     FEEAGVFSKITLFDRQPDLILTGRINALYEHYHPRVWTKLPLPYVGKMAAGILDLKTH
                     SSSGEADLTLFLLTKSGELIGTYRGSSSFNENFNPTGEMAPGARLNRALAEAVQKIQD
                     QMLHDSHLRTIASR"
     gene            complement(6529..6843)
                     /locus_tag="VFR82_15155"
     CDS             complement(6529..6843)
                     /locus_tag="VFR82_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502986.1"
                     /translation="MRIVFYSMLTAFIAINMTLAGCAKNASERRSASPTMSERISKDS
                     VKGRVSEIGAKYVSIRDNDGESRRVRVDDNTKMDQVAVGDQVKAYVTEDGYASTIQRV
                     ID"
     gene            7149..8273
                     /locus_tag="VFR82_15160"
     CDS             7149..8273
                     /locus_tag="VFR82_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4502987.1"
                     /translation="MRIAQVSPLWESVPPRLYGGTERIVSYLTEELVRQGHEVTLYAS
                     GDSQTSARLEAACPHALRLSTGLVNRDAPLILMQEHAMGADADQFDIIHSHLDFLSFP
                     MSRRCPTPVLTTLHGRLDLPELGPIFREYADMQLVSISDAQRLPLPHANWQATVYHGL
                     PDLYTFHPNTGSYLAYLGRICPEKRPDHAIEIAKRVGIPLRIAAKVDPVDREYFVTKI
                     APLMDHPLIEYLGEISDAEKGHFLGDAAAVLCTYDWPEPFGIVLIEALACGTPVFAYR
                     RGSIPEIIEDGVTGFICDSLDEMVTKIDRLPLIKRQRCRESFQARFTVERMVKDYLAV
                     YERMATVPARSHAIANGRSLRMVGPRPVAVSELTTPHGTA"
     gene            8516..9055
                     /locus_tag="VFR82_15165"
     CDS             8516..9055
                     /locus_tag="VFR82_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502988.1"
                     /translation="MTMLLDDVAVPQHVRRLAVWYPRTWEQEVSYGYSRLEQAAFQLK
                     KRRADIKIVERRNLEQLTDEQRLQLSGRVGDDSVVRVGRWLGADGLVLFRIDGPTWRE
                     RLLARMHGKMPPFVVSSKIISLESGEVLYHDVVTAVPVPDTGEWSDYDSDHQLRPAMH
                     AALDQALSTAIQHLHQSFR"
     gene            complement(9072..9542)
                     /locus_tag="VFR82_15170"
     CDS             complement(9072..9542)
                     /locus_tag="VFR82_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502989.1"
                     /translation="MSVPTASPLLPSTTERVPQHTDPAINERITQQTEANVQVYSTDR
                     RLIDVRLEELDGEWDIERILEANAAAVSLIGLTLARFVNRRWFLLPAAVATFLLQHAV
                     QGWCPPVPLFRRLGIRTQREIDEERCALKAMRGDFLTAKAGSSSAQEVLRAVRS"
     gene            complement(9638..10147)
                     /locus_tag="VFR82_15175"
     CDS             complement(9638..10147)
                     /locus_tag="VFR82_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502990.1"
                     /translation="MDILSHGLWGGIAFGRKSRRSFWLAFAIGLAPDLLSFGVLWIAV
                     ILGLADPPDFSHGTPPESTIPQYVHHLYDITHSFVVFLFVFLLIWVATKRPRWEVAAW
                     GLHVLVDVPTHSHAFFATPVLWPLVGWKFDGWQWMTPAILIPNYVLLALLYAWFLSRF
                     ARTVPGRAN"
     gene            10348..11568
                     /locus_tag="VFR82_15180"
     CDS             10348..11568
                     /locus_tag="VFR82_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502991.1"
                     /translation="MAQPGGGTRDVVKAVFLIVLLVALPLIGVILAGKPIDQYLEFPP
                     HTRYVEHPPFSWQTFVILGFVTVLSVSPIIVRVVTSQVTARGSVRTAKAYPWWGVVGA
                     ALTAVSWWLAWTRFEWFKSYQASTFTPLWLGYILFVNAWTYTRTGRCIMLDQPRIFLW
                     LFPVSAGFWWSFEYLNRFVQNWHYVGAQTFTSWDYFMQATVPFSTVLPAVLSTRELVG
                     TFPRLSAGMDRLPAFHIRHLHIAGWGVFILACAGLVILGIWPNYAFPLVWLAPLALMT
                     SLQMLIGEETIFSATPGGDWRSLWWSALAALVCGICWELWNYKSLAHWEYAIPFVHGY
                     KIFEMPLLGYAGYLPFGLECLAVAKFLFPDHDMERMTPKTESSSPVDDQRLAGGEPVM
                     SADQFRAEAGYFKV"
     gene            complement(11553..>11912)
                     /locus_tag="VFR82_15185"
     CDS             complement(11553..>11912)
                     /locus_tag="VFR82_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4502992.1"
                     /translation="AVPDYPQLVKGPYKSAYFEIDVDFSKTDMVRVKELQRHLAVDYV
                     YMLWAPATVSHGHSTWFSKNYLVTQIVAQLFESPGGKEIGRSQYAVRVDDETHQVFSD
                     DMRRVALELAEKTHTLK"
BASE COUNT         2572 a         3274 c         3421 g         2645 t
ORIGIN      
        1 ctctgcaaca ctgcggctcg tggcatggag catttgcgcg ccgagctgaa ggcgccagcg
       61 ggcgaggtag gccatcggcg tttccgatag atattgccga aagcgctctg ccagtacgga
      121 acgcgacatc cccacctccg ccgcgagtgt ggcgattgtc catggacggg ctggttgacg
      181 atgcaggaac gccaacgctt tcccgatttc cggatcgcga acaccggcca gccagccggc
      241 ctgtttctgt gggagctgct cgatgtaacg ccgcaaggtt tcgataaaca agacctccga
      301 caacttcgcc agcacggtct caccacctgg tctggacgca tcggaattgt caaccgaata
      361 gcggagagag ttttccaacc attcgccgga ggcgtcattc cggatactga ccttcaagat
      421 gggcggcagt ccgctgagaa gaatcctgct cagctgcggc tcgcaggcca aatatccgca
      481 gatgattctc gttaattctc cgcctccgcc gtgccgatag atcttcaggt cctcagacag
      541 aatgcgttgc agttcttttg cgctgtcgac cggaacgatg ggcgaaccat tgccgaggat
      601 gtggggatga ccgtgtggaa agactacgat ttctcctgcc tcgagaggga ccggcctggt
      661 gtcatcttcg atgtgtgcat agccgcggcc ctcggtcacc aaatgataga cgattacgtg
      721 tttaggttgc ggcgacagat aggaggccat gacgcgtgag ggaggcgagc gaaagcccca
      781 cggtgcagaa cattcagcgg tgtagaagat ggccccgtcg agtttgacgg ccttgaggac
      841 ttcggacaac acatccacag aatgtgcctc tttagggcgt ccggcaaact aaaccggacg
      901 gtcggtcaaa tgatgcgcag cctagcacgc tcgcgtcaag agaaccagac gctcggataa
      961 gcacaaccgt aagggatcac cttatagtgg agacacatca gcagcggcaa caacggtgct
     1021 tcaatcagat cactcacaaa ggaggccaac atggcgacaa tttcactcac accggatgta
     1081 gagacattaa agacgcggct tcggacgatg tggatggcgg gcgactacga tcgattctcc
     1141 cgctacatgg aagccagcgc cagggaattc tatgaccgca ttccactttt cccgggctgc
     1201 acgttcctgg atgtcggttg tggctctggt cagctggcgc taatggcggc aagggatgga
     1261 ctacatgtga ccggcgtcga catcgcggcc aatctcgttg aacgtgccag gagccgggcc
     1321 caggccgaag atctgcaggc tcgattcgaa gaggccgatg cggaggcact gccattcgag
     1381 gacgaaagtt tcgatgtggt ggcaagtctc atcggcgcga tgttcgcgcc gcagcctgag
     1441 gtggtcgccg gggaattgat gcgtgtctgc aagcccggtg gaacgattgc gatggccaat
     1501 tggactccac aggggttcgt gggacaaatg ttcaaagcag tttcgaaatt cattgcccca
     1561 tccggaatgc catccccggt tttgtggggc gacgaagcaa cggtgcgtga acggttggga
     1621 cacggactca cggaactccg cctgaccagg cgtcagtatg ccttcatgta tgatttttcg
     1681 ccggcggctg tggtggagct atttaaactt tattatggac caatcaatca ggcattcgga
     1741 gcgcttgatg ctgcggggcg tgacagcctt cgcggagagc tcgaagggtt gtggtcggct
     1801 cacaaccgtg cagggataga ccgcaccacc gtgtatgcgg aatatttgga ggtcatcggc
     1861 attcgagcat gaggcaagaa ccgccgtatt catacaaaga ggctttcgtg tacgacatga
     1921 gaagccgcat ggcgtgacgg ctcgatttcc cagaattaca tggaacggct cgccgtgggg
     1981 ggttgttcac catcccaacg tccctattgc atcgcgtttc agtggaacgg gcaagaaagc
     2041 tggacgcgcg ggatcctgaa atcgccgaag ccgtgcgtgc ggtggggcgc cgggggcagg
     2101 taatcaatca gcggccgact taggagaatc cccagtaggg ggtgacgaaa ccagaccgta
     2161 tcatttttat agagactggt attcgccaac ccaaaggagg gacaagttat gaggatcttg
     2221 acattcggtg cattcttgat cgccggcgca tgcctggttg ctgcggatta tacgcttgct
     2281 caggcaggat cgggaggctc tggcggggca agtggaagcg gcatgacctc tcagggtctc
     2341 gcgtctgaac ctgccggtat aggtgagcga ggaactgggc aaacccagag cacgcttgag
     2401 cgatccggag ttcccgccca gaaaggaatg tcctctgaac ccgcaggaat tggagagaag
     2461 ggtaccggac agacacaaag tacgatggaa cggtccgggc ttggacagaa caatgctcaa
     2521 agtcaatctc cacttccctc cggtatcggt gaacagggta ccggccagac gcagagcaca
     2581 atggagcggg ccggtggagg acaaggcgcg acccaaggcc aatcgtccca aagcgcaatg
     2641 gatcgtccag aacgttgacg agtcggattc acttcgcgcg tggaggtgcc ggacttctcc
     2701 ggcgcctcca gcgctgcttc tccacaaact tcatcatatt ccagttgttc tcgggcatga
     2761 tcgtgcgcat cctccatgca ttctatcttg ggcgagcgtg acgtgagttt gctggctggc
     2821 tgtcgccgac gattgactat gttgtgaaga ccagggaatt tcctcggttg acacgcttac
     2881 tgacaaagcg tacacgtgaa attgctgact cccgatattc cttccgaacg atggaggagg
     2941 tcatcatgcc cgacctgaat ccaccggacc atcaccgccc tcccagtcct gggtgggctt
     3001 ctcgtcagtc acgctagcgg gtcttcatca gccgccgcgg tcccgcgatg ggactattta
     3061 gatagacttg aagggcgcag tgacggagac ggggactagc gtgaggaaca caacatctgg
     3121 aattccagtt gactgtccgc tgacgagctg ccctgttcct catggcgagg gagggtcctg
     3181 tgtgcacaca taacgaatct acctcggcgt cctaagcgcc acaaacgggc ggccttcgca
     3241 tgtcatcgag gtcgcccgtg ttgtatgtca gatattgcgt gtctatgtcg tattagtaga
     3301 cgcgtagtgt cagtctagta cttccacctg gtccattgtc cgagcgtcga caagatcatc
     3361 tcatgaatgg caagcacctg gttcttgcac acgaatgctg cggcaccggc ggttctcatg
     3421 gcgctgtacc tcggggggga aaagtggccg gtaaggcaga taactacagt ctccggacag
     3481 gatttcctga tacactgagt cgcttcaact ccgtcaagat aggggaggcc gatgtccatt
     3541 agaatgacat cgggcttgta ttcgatagcc attgccacag cctgctggcc gtctccggcc
     3601 tgccccacga tcctaatatc ctggtatttt tcaaggatgt cttcaaggag ctggcgcgtc
     3661 ccgggatcat cgtcgacgag gagcacacgg tattgaactg gccgggcgtc agtcgaagat
     3721 gtcgacacta attgacttcg agaagccaag tcattctgga cgccatgatt gccctctact
     3781 gaaacgagca taacgacctc tccttaacaa taacaatcac atcggtgtga tagatgcaca
     3841 cggttgccag tgcatgacag actctccatc acctttatct ctcttataaa gcgatcgatt
     3901 atgaacgcgg cctagtaata acccaagtct ggcccgtttt tttccttcat gccgcaccct
     3961 cgagttagtg cgtgcggcaa ggccacgaaa ttcctcaaat cctgatgcac tttttgatcg
     4021 aggttaattg cgtagcttcc atccgtcaaa tactgatcga agaagcgaaa ggcgacattc
     4081 aaggaacatc tgttctagtc atcggagccg cgtgtcttcg tgaagcactc gatgtgggca
     4141 tgacgaaatc gctgcctggg gcaacgcgct tcaagaaatg atgagtaaca agcgattgag
     4201 gaaaggtata gagcgcaggg tcagaaaatc atgtccgtcg gcttgcttcc ataggccaag
     4261 cggatgctcg gggaattcct tgaaaagtgg tgtggtgtcg tattgaatca aatcgccccg
     4321 gcaactctgt gacagatttc aagtctgtta ccgctcggat cttcgaaaaa tagtgcgtaa
     4381 tagccggggc cctccaggta ggcgggtcct tcaatctgac gacctcccgt ttgaacaaca
     4441 atggaagcta aacggtcgac ctcggcggta ctctcagccc agaatgcaac tcgacattca
     4501 ttggctctat gcccaggggc ctcggtgacg ccaaaaaatt ccgtcgatcc acccgctcca
     4561 ccaaccgctt caaattgcaa ccacccgtcg acctctatcc ggcgggtgaa tcccaacgcg
     4621 ggcaacagag cctcataaaa cacgcgtgcc tccggcaggc tccgcacccg aagatcaatg
     4681 tgatcatatc gccttcgcat cgatttcgat caaaagtagg cgctatattc tacttgcccg
     4741 ttgttcgacg gtctatcgtc taggttcctc cccagtccct gaggcgatga ccagtcagtt
     4801 atgcttgcac tccattatgt atggacacat cacctagtat gtttcggttg atacaacgcc
     4861 gggcctcatc catgccgatg cgcgtgctcg gaaagaccgg ggtgaacctg cccatcctgg
     4921 ggttcggcac tgcacatagc ggccttcgct tgaacaccag agaggcggtt catctctatg
     4981 aacgagcctt cggagaaggc attacctatt ttgataccgc gccagagttt gcaggctacg
     5041 gaaaggctca aatgcagttg agccattttc tcaagcaggt gcggccacaa gtcttccttg
     5101 tgacgaagtg ctatgaaccg gatggtgagc gggccttgca gctgctgcag aaaagcctga
     5161 aggaactagg caccagttat gccgacttgg tgttcgttca cagcgtcggc gccgacaaaa
     5221 tggatcctcg aacggtgttt ggcaacaaag gcacctacgc tgcattgatg aaagccaagg
     5281 cgctcgggct gacacggtat gtcggtttgt caggacacag ccgcccgggg cgcttcgcgc
     5341 aggcgatacg ccaattcgac gtcgatgtgt tgttgaacgc cgtcaatttc gtcgaccagc
     5401 atacttacaa tttcgaagcc gaggtgtggc cggcggcggc gcgggcaaac atcgggctca
     5461 tcgcgatgaa ggtgtatggc ggcgcgcagg atccgccacc gcgaccggac cggctcagtc
     5521 attcgatgat ggcggccgaa catcacgatc ttgccttccg gtatgccttg agtctgccac
     5581 aggtctgctg cgccgtaatc ggcatggcca caactcgcga gctcgagcag aatgttgcgc
     5641 gtgcacgggc attcaaaccg ctgtcgggcg gtgaggtgag aagcctgcga gacgccggca
     5701 ggctgatggc ccgccaatgg aacgatcatt ttggaccagt cacgtaactg cgagtcgtga
     5761 gaaatcccgg agagtgaaca gccacccttc gatgacctcc cccttcttcg tcattcgtaa
     5821 tgcgtttcgg cagtggtctc tatctggctc tatctcggcg gtcagcgtga cgcgattgtt
     5881 ctcagatggg agtcatgcag catctgatct tgtatttttt gtaccgcctc ggcgagcgcg
     5941 cggtttagcc gcgcgcctgg ggccatttct ccggtcggat tgaaattctc attgaatgag
     6001 gaagaaccac gataggtgcc gatcagttca ccgctcttgg ttaatagaaa gagcgtgaga
     6061 tcggcctccc cgctgcttga gtgcgttttc aagtccagga taccagccgc cattttgccc
     6121 acgtagggca aaggaagttt ggtccacaca cggggatgat agtgctcgta taaggcgttg
     6181 atccggccgg tgagaatcag gtccggctgc cggtcgaata gggtgatttt cgaaaacacc
     6241 cctgcttcct cgaatgcatc caggagctcg tgttgcactt gattcgccag cacacgaggc
     6301 ggaactgctt ccgcgtccgg ccctgtgaga ccgaagtttt ctttgcccga caccagcgcc
     6361 ggggccggat acatgtcgtg gaattccgct gtggcgtcaa tgatcggccg tgccggggcg
     6421 agcgtgatgg cggggacaaa ttcggctttg ctgcagcctg caacggcagt cgctagaatc
     6481 aacacataca ggaaagcgga tcccctgtga ctcaatccct taggctcttt agtcaatgac
     6541 gcgttgaatc gtcgatgcat agccatcttc cgtgacgtag gctttaacct gatcacccac
     6601 agccacttgg tccatcttgg tgttgtcatc gacgcgtaca cgcctcgact ctccgtcatt
     6661 atctctgatc gagacgtact tggctccgat ctcactcacg cggcctttaa ctgagtcttt
     6721 agagatgcgc tcgctcatcg tcggcgatgc ggaccggcgc tcagacgcat tcttggcgca
     6781 acccgcaagt gtcatgttga tcgcgataaa ggccgtaagc atcgagtaga aaactatcct
     6841 cataggacac ctccttagcc ttacttttga atgtacccgt cagggcgtag ttggcctccc
     6901 taggtacgtc actaggatcc aagtctcctc acccaagcac tgaagtaatg tagggttttc
     6961 acttctatga ttgaaggcat gcgtagctag cgaaacctct agctttcgga aaaccccttg
     7021 ctctatgccg gcttgcgtag gcaatgaggc ggcatgtggc gtgcaatcct ttctcaccag
     7081 ctagcttcga tgcctcgacg gtggatgaag ggtaatgaaa ttaagagaaa caagaaagga
     7141 agattgacat gagaatcgca caagtctcgc ctctgtggga aagcgtgcca ccgaggctct
     7201 atggaggcac cgagcgcatc gtctcgtatc tgacggagga actcgttcgc cagggccacg
     7261 aggtgacgct ctatgccagc ggtgattctc agacgtcagc ccgtctcgaa gcggcttgtc
     7321 cgcacgccct gcgtctgagc accggtctgg tcaatcgtga cgcgccgttg attctgatgc
     7381 aggagcatgc aatgggcgcc gacgccgatc aatttgacat catccattcg catctggact
     7441 ttctgagttt tcccatgagc cgtcgttgtc caacccccgt gctcacaact ttgcatggac
     7501 gcttggacct gccggaactg ggtccgatct ttcgagagta tgcagacatg cagttggtgt
     7561 ccatttcgga tgcccagcgt cttccgcttc ctcatgccaa ttggcaggcg accgtgtatc
     7621 acggattgcc cgatctctat acatttcatc ccaacaccgg atcctatttg gcatatctcg
     7681 gacgtatttg tcccgaaaag cgccccgatc atgcgattga aattgccaaa cgggtcggaa
     7741 ttccgttgcg aatcgcggcg aaggtggatc cggtggaccg ggaatacttc gtgacgaaga
     7801 ttgcgccgtt gatggatcat ccgttgatcg aatacctcgg cgagatcagc gatgcggaaa
     7861 aaggccattt cctcggtgat gccgcggcag tgctgtgcac ttacgactgg cccgagccct
     7921 tcgggatcgt cctgatcgaa gcgctggctt gtggcacccc cgtgttcgcg taccgtcgcg
     7981 ggtcaattcc tgaaatcatc gaagatggtg tgactggatt catctgcgac agcttggacg
     8041 aaatggtgac gaagatcgac cgcctgccgc tcattaaacg gcagcggtgc cgtgagtcgt
     8101 ttcaagcccg tttcaccgtc gagcgaatgg tcaaagatta tctcgccgtg tatgaacgga
     8161 tggcgactgt gccggccaga tcccacgcga tcgccaatgg acggtccctt cgcatggtcg
     8221 gtccccggcc tgtcgcagtc agcgagctga ccaccccgca cggtacagcc taagcacaga
     8281 aagccgggtc caaccatatt ggacccggct tttttcttac cttccttcct ctcatatctt
     8341 gcctatcgta ggaaggctcc acccttccgt aggatagcgg tcgtcctgcc cggcctccta
     8401 ctatggcatc atgcgtgcct ccactaacgg ccggcgagta ctctctgttt tcctgatggc
     8461 gatcgttttg ctgcccacag cctgtcggcc cgtgccgatc gctgaccact cccccatgac
     8521 gatgctcttg gacgatgtcg cagttcctca acatgtccgg cgcctcgccg tttggtatcc
     8581 acgcacctgg gaacaggaag tctcgtatgg gtacagcagg ctcgagcaag ccgcgtttca
     8641 gctcaagaag cggcgggcgg atatcaaaat tgtggagcgg cgcaacctgg aacaactcac
     8701 ggatgaacag cgtttgcaac tatccggacg agtcggtgat gacagcgtcg tgcgagtggg
     8761 caggtggttg ggcgctgacg gcctcgtcct cttccgcatc gacgggccca cctggcggga
     8821 acgattgttg gcccgcatgc atggaaagat gccgcccttc gtcgtctcga gcaagattat
     8881 ctcactggaa agcggagaag tgctgtacca tgatgtcgtg acggcagttc cggtgccgga
     8941 taccggtgag tggagcgatt acgacagcga tcatcaactg cgtcccgcaa tgcatgctgc
     9001 acttgatcag gccctgtcaa cggccattca gcatcttcac cagtcatttc gttaaagttc
     9061 cgcccccgat ctcagctgcg aacggctcgc agcacttcct gtgcggaaga cgaccctgcc
     9121 ttggcagtta gaaagtctcc tcgcatcgct ttcaaagcac aacgttcctc gtcgatctca
     9181 cgctgggtgc gaatcccgag acgacggaac aagggcacgg gtggacacca tccctgcacg
     9241 gcgtgctgga gcaaaaaggt ggcaacggcg gctggaagta agaaccaccg tcggttcaca
     9301 aagcgtgcga gcgtcaatcc gatcaaagag acggccgcgg cgttggcttc gagaatccgt
     9361 tcgatgtccc attctccatc cagttcctcc agcctcacat caatcaggcg tcgatcggtg
     9421 gagtacactt gtacatttgc ctcggtctgc tgcgtgatac gctcgttgat tgcaggatcg
     9481 gtatgttgcg ggactcgctc ggtcgtggaa ggcagaaggg gagatgcggt tgggacggac
     9541 atggaggcct ccatcaaggt gcagttgagg ggatagaatt gcccattctt gaggagactg
     9601 cacctcgggg cttccccaga gtgaggtcag cgatgcgcta attggctcgg ccaggaacgg
     9661 tacgagcaaa ccgagacagg aaccacgcat agagcagcgc gagtaagaca tagttgggaa
     9721 tgagaatggc tggcgtcatc cattgccacc cgtcgaactt ccaaccaacc aacggccaga
     9781 gaactggagt tgcgaagaag gcgtgggaat gagtcggcac gtcgaccagc acgtgcaggc
     9841 cccatgcggc gacctcccac cgcggccgct tcgtcgcgac ccagatgaga agaaagacga
     9901 agagaaagac gacgaagcta tgcgtgatgt catacaggtg atgcacatat tgtgggatgg
     9961 ttgattccgg tggcgtacca tggctgaaat cgggtggatc tgccaagccg aggatgacgg
    10021 ctatccatag gactccgaaa gacaacaggt ccggtgcgag gccgatcgca aaggcgagcc
    10081 aaaagctgcg gcggcttttt cggccgaaag cgatgccgcc ccaaaggcca tgtgaaagta
    10141 tgtccatgac ggctcggagg tagagcgatc gatggtacca taaccaattt tcccccgact
    10201 tcccgcgaag ccggacgagg aaagttgccg gctagtcaac cgctcattca gtaccacaaa
    10261 tgcgcgattg tggaggaaac caacgctgag gagcaaactt cgaagttaac acccagctca
    10321 gttgagactt gtgaaggcgg agaaagcatg gcccaacctg gcggcggcac acgtgatgtc
    10381 gtgaaagccg tgttcctgat cgtgctcttg gttgcattgc ctttgatagg cgtgatactt
    10441 gccggcaaac cgatcgatca gtatctcgag tttcccccgc acacccggta tgtagaacat
    10501 ccgccgttct cctggcagac gttcgtcatc ctgggattcg tcaccgtgct gtcggtctcg
    10561 ccgatcattg tccgcgtcgt gacctcacag gtcactgcac gtggcagcgt ccggacggca
    10621 aaagcctatc cctggtgggg agttgtcggc gccgctctca cggccgtctc ctggtggtta
    10681 gcctggactc gcttcgaatg gttcaaatcc tatcaagcat ccacgttcac gcctttgtgg
    10741 ctggggtaca ttctgttcgt gaatgcgtgg acgtacaccc gcacgggacg ctgcattatg
    10801 ctggatcagc cgcgcatctt tttgtggctg tttccggtca gcgcgggatt ctggtggtca
    10861 ttcgagtacc tgaaccgctt cgtgcagaat tggcattacg tcggcgcaca gacgtttaca
    10921 tcatgggatt attttatgca ggccacggtg ccgttttcga cggtccttcc ggcggtgttg
    10981 agtaccagag aattagtggg tacgtttccc cgattgagcg cagggatgga ccggcttccg
    11041 gcgtttcaca ttcggcacct gcacattgcc ggctgggggg tgtttattct ggcttgtgcc
    11101 ggactggtga tcttaggaat ttggcccaac tacgcttttc ccctcgtatg gctcgcgcca
    11161 cttgcgctga tgacgtcgct tcagatgctt atcggcgagg aaacgatctt ttcagcgact
    11221 ccagggggtg attggaggtc attgtggtgg tcggcgttgg cagcccttgt gtgtggaatc
    11281 tgctgggaac tgtggaacta taagagtctg gcgcattggg aatacgccat tcccttcgtg
    11341 catgggtaca aaatttttga aatgccgctt cttggctatg ctggctatct tccgttcggc
    11401 ctggaatgtc tggccgtggc caaatttctg tttcctgacc atgatatgga aaggatgacg
    11461 ccgaagacgg agtcttcttc tccagtagac gatcaacgtc tcgcaggtgg tgagcccgtg
    11521 atgtcagcgg atcagtttcg cgcggaagcg ggctatttca aagtatgagt cttttcggcc
    11581 agctcgagcg ccacgcggcg catatcatcc gagaagactt gatgcgtttc gtcgtcgacc
    11641 cgcaccgcat actggcttcg tccgatttcc ttgccgcctg gggactcaaa cagctgggcg
    11701 acaatctgcg tcacgaggta gtttttcgaa aaccaggttg agtgtccgtg cgagacggtg
    11761 gccggtgccc agagcatata cacataatcc actgcaaggt gtcgctggag ttccttcacc
    11821 cgcaccatgt ccgtcttcga gaaatcgaca tcgatttcga agtaggctga cttgtacggt
    11881 cccttaacca actgcggata atcgggaaca gc
//
LOCUS       DASTQY010000473         4045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1386757, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000473 DASTQY010000000
VERSION     DASTQY010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386757"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VFR82_15190"
     CDS             complement(<1..794)
                     /locus_tag="VFR82_15190"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4502993.1"
                     /translation="MAKSTMTAWLRLAGTLIRSHGWQRPFRTVLTVAGVALGVLSFVA
                     ILTANREVLRAFERAVLTVAGPATLEVSGRDLGLDETILEVIRHSAGVQKVIPLIEEA
                     VVLGEGTSAGESVQIYGMDLLEEAEGIGFRVERAETEDLLNDLLEPDRVFLGRQLSDE
                     LNLVPGSTFKARAGSRWMHLKVAGLLEGSDRPKSYWNRVAIMDLAAAQVLFQMVGRLD
                     RIDIVTAPNRTPEEVAASLRAVLSPSVVVQRPTQRTAQIEKMVGAF"
     gene            953..1219
                     /locus_tag="VFR82_15195"
     CDS             953..1219
                     /locus_tag="VFR82_15195"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4502994.1"
                     /translation="MKTALRRKPDTLIEEPVDLELLTPRQRDILRLVSIGHTNREIAN
                     MLQISVRTVEVHRFNLMRRLNVRNVAQLLRRALQLGLLSKSFGK"
     gene            complement(1298..2224)
                     /locus_tag="VFR82_15200"
     CDS             complement(1298..2224)
                     /locus_tag="VFR82_15200"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU4502995.1"
                     /translation="MDLTVDIAGVKFPSCFMNASGALCVTEDELMALGRSHSGAIVTK
                     SMTIEARQGNPMPRYHGFSGGSINSMGLPNLGYRAYAALIPALKQFGKPVIASVAGLC
                     EDDFPTIAQVINDAKPDLIEVNLSCPNIPGKPQIGYDADTSERVIKRVRKIVTVPMGV
                     KLPPYFDPIHHQLMGDVIARCGVNFLNLINSVGNGLVVNPHTETVVIKPKGGFGGLGG
                     AMIKPVALANVRAFWKHFQGNMPIVGTGGVVDGVDAFEHVLCGASAVQIGTVLVEEGV
                     QVFARLEHELTACLEKQGYKSIADCRGKLKEL"
     gene            complement(2259..2975)
                     /locus_tag="VFR82_15205"
     CDS             complement(2259..2975)
                     /locus_tag="VFR82_15205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4502996.1"
                     /translation="MLPPLMSPIVKLTEVSKLYERGDDRICGLDRVTLEVTAGAFCAF
                     VGPSGCGKSTLLNLIAGLDLPTSGDIQLIGQSTAHYNADDWTRVRRQCIGIVFQAFHL
                     VPGLTVEENVAFPLLLRGDESRRVGERVSEVLALVRMNHRARHRPSELSGGEQQRVAV
                     ARAIVHQPRIVLADEPTGNLDSQHGGEIIALLRTLARDFHLTVLLVTHSAAAAEAADY
                     IWAMKDGRLLTRTVLKAASC"
     gene            3074..3868
                     /locus_tag="VFR82_15210"
     CDS             3074..3868
                     /locus_tag="VFR82_15210"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU4502997.1"
                     /translation="MLRWCRLALRIVLPVDCINCGLPLRTDPTPCFCDACWQRIHPLS
                     GPRCSRCDQPFVAAAATSWTPDHTCQHCREQPPAYHRAWTIYPYIPPLQEAICAMKYR
                     GVSALAGPLAALMIRALPPDIECDVVVPVPLHPSRLRVREFNQSLLLADRLGRFFMRP
                     VSTTSLIRTIATEPQTALTRRERLRNLRCAFAVRDAKPLAGRRILLVDDVYTTGTTLN
                     ECTKTLLKAGALSVSALTLARTMDSSLVPDRIWAEHAGRSLTGFGL"
     gene            3873..>4045
                     /locus_tag="VFR82_15215"
     CDS             3873..>4045
                     /locus_tag="VFR82_15215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEU4502998.1"
                     /translation="MPIYEYRCAECQKRSSFLVLNRSTETSLCCSHCQSPRLERLLSR
                     FASPKSDEARMES"
BASE COUNT          848 a         1262 c         1067 g          868 t
ORIGIN      
        1 tgaaacgccc ccaccatctt ctcgatctgc gccgtacgct gagtcggccg ctggaccacg
       61 acggaaggcg aaagcaccgc tcgcaacgaa gcggccactt cctcgggcgt tcgattgggt
      121 gcggtgacga tgtcaatccg atccaatcgg ccgaccattt ggaacagcac ttgggccgcc
      181 gccaggtcca tgatggcgac gcggttccaa taagatttgg ggcggtcgct gccctctaat
      241 agccccgcca ccttcaggtg catccaacgc gagcctgccc tggccttgaa cgtgcttccc
      301 ggaacaaggt tcaactcgtc gctcaactga cgcccgagga acacacggtc tggctccaag
      361 agatcgttca gcaggtcttc ggtttcggct ctctcgaccc gaaaaccgat gccctcggct
      421 tcctcgagaa gatccatccc gtagatctgc acgctttcac cggcagacgt gccttcccct
      481 aggacaaccg cttcttcgat gaggggaatc actttttgga cgcccgctga gtgtcgaata
      541 acctcgagaa tcgtttcatc gagtccgagg tcgcgccccg acacttccag agtcgccggg
      601 ccggcgacgg tcaagaccgc tcgctcaaag gctcgaagca cttctcgatt ggccgtgaga
      661 atcgcgacga aagacaatac cccgagcgcg acacccgcca ccgtcagtac agtcctgaac
      721 ggccgctgcc atccatgaga tcgaatcagt gtgcccgcca acctgagcca ggctgtcatg
      781 gtcgatttgg ccatacgggt aagtgcgtag ctactaattt gttgagacgg cgctttgcaa
      841 gcgtggatgc ggcaccgcta ggatctgcac aaatcggcag tttcgtttac agagtgccat
      901 tcctttgcta gactcgcctc aagttatcga tggctatcga aggtgggaaa acatgaagac
      961 cgcgttacga cgaaaacctg acacgctgat cgaggaaccc gtcgacctcg aattgctgac
     1021 gccccgccaa cgagacatcc tgcgcctcgt ctctatcggg catacgaatc gcgagattgc
     1081 aaatatgtta cagatcagcg tacgtacggt ggaagttcat cgcttcaatc tcatgcgacg
     1141 attgaacgta cgcaatgtgg cacaattgct cagacgagcc ctccagctgg gcctgctttc
     1201 caaatccttc gggaaataac ctcgcgcgtt ctacgacccg ctcgaatgca ctcaacctca
     1261 gagcttcctt cgattcttct ccggtgcgcc agctctgcta caactccttg agcttgccgc
     1321 ggcagtccgc aattgactta tagccttgtt tttccagaca ggccgtgagc tcgtgctcca
     1381 atcgtgcaaa gacttggacg ccttcttcaa ctaataccgt cccgatctgt acagccgagg
     1441 cgccgcagag cacatgctcg aaggcgtcaa cgccgtccac gacaccgccg gttcccacga
     1501 tcggcatgtt gccttgaaag tgtttccaaa aggctcgtac gttggcgagt gcgaccggtt
     1561 taatcatggc tccgccgagt ccgccaaagc cgccctttgg tttgatgacc acggtttccg
     1621 tgtgcggatt tacgacaagg ccgttgccaa cggaattgat caaattcagg aaattcactc
     1681 cgcaacgggc gatcacatca cccatcaatt ggtgatgaat cgggtcaaaa taaggcggca
     1741 acttcacccc catggggacc gtcacgatct tccgcacccg tttgatgacc cgttccgagg
     1801 tatcagcatc gtagcctatt tgcggtttgc ccgggatgtt cggacaggaa agattcacct
     1861 caataagatc cggtttcgcg tcattaatga cctgcgcaat ggttggaaag tcgtcttcac
     1921 acagaccagc cacactggca atgaccggct tgccgaattg cttcagtgca gggatcaatg
     1981 cagcataggc cctgtacccc aggttcggca gtcccatgga gttgattgaa cctccggaaa
     2041 aaccgtgata gcgcggcatg ggattgcctt gcctcgcttc gatcgtcatg gacttggtaa
     2101 cgatggctcc agaatgagag cggcccaatg ccatgagttc atcctcagtc acgcacaagg
     2161 caccggacgc gttcatgaaa cacgagggga atttcacgcc ggcaatgtcg accgtaagat
     2221 ccatcacagt cctactgcac tctggtcacc cactccattc agcaacttgc cgctttgagt
     2281 acggtgcgcg ttaacaatcg tccgtctttc atcgcccaaa tataatcggc ggcttccgct
     2341 gcggccgcac tatgcgtaac cagaagcacc gtaagatgga agtctcgagc caaggtgcgt
     2401 aggagtgcga taatttctcc cccatgctgc gagtccaggt tgccggtcgg ttcgtcggcc
     2461 aacacaatcc ggggctgatg cacgatcgcg cgtgcaaccg ccacccgctg ttgctcacca
     2521 cccgacaact cactcggtcg atgtcgcgcg cgatgattca tgcggacgag ggcgagaact
     2581 tcggaaactc gttcaccaac tctccggctt tcatcgcctc gcagcagcag ggggaaggcg
     2641 acattttctt caacggtcag accgggaaca agatgaaaag cttgaaagac gattccgatg
     2701 cactgacggc gaactctcgt ccaatcatcg gcgttatagt gggcggtgga ttgtccgatc
     2761 agttgaatgt ccccggaggt cggaaggtcg agccctgcaa tcaaattgag caacgtgctc
     2821 ttgccacatc cgctggggcc gacaaacgcg cagaaagcac ctgctgtcac ctccaacgtg
     2881 acccgatcca acccgcagat ccggtcgtcg ccccgttcgt agagcttgga cacctccgtc
     2941 agcttcacta tcggcgacat taacgggggc aacattcgtg tcagacctcg tgttctatac
     3001 catacccctt cacggagcga caaccttgac agcggtcccc ggttctccta ccttcaacct
     3061 gatgccttac cgcatgctgc gatggtgccg gctggcgctc cgcattgttc ttcccgtcga
     3121 ctgcattaat tgtggcctgc ctttgcgaac ggatcccacc ccttgtttct gcgacgcatg
     3181 ttggcagcgc atccacccgc tctcgggccc gcgctgctcc cgctgcgatc agccgttcgt
     3241 ggctgcagct gccacgtcct ggacgccgga tcacacgtgc cagcactgcc gagagcagcc
     3301 tcctgcctat catcgggcat ggacgatcta tccttacatc ccacccttac aggaggccat
     3361 ctgtgcaatg aagtatcggg gcgtatcggc gctggctggg cctctggcgg cattgatgat
     3421 tcgagcactc ccgccggaca tcgagtgcga cgttgtcgtc cccgttccgt tgcacccgtc
     3481 gaggctgaga gtccgagagt tcaatcaatc gctcttgctt gccgaccggc tagggcgctt
     3541 tttcatgcgg ccggtatcca cgactagcct catccgcact attgccacag agcctcaaac
     3601 ggcgctgacg cgccgtgagc gtttacgcaa tcttcgctgc gcatttgccg tgcgggacgc
     3661 aaaacccctc gccggccgtc gcatcttgct ggtcgacgat gtctacacca ccggaacgac
     3721 gcttaacgag tgcaccaaga cgctgctaaa ggccggggct ctctcagttt cggcgctcac
     3781 gctcgccagg acaatggact cgagccttgt accagaccgt atttgggctg agcacgccgg
     3841 ccgttcgttg acaggattcg gactttgaca cgatgccaat ttacgaatat cgctgtgcgg
     3901 agtgccagaa gcggagcagt tttctcgtcc tgaaccgctc cacggaaacc tcgctctgct
     3961 gctcgcattg tcagagtccc agactcgagc ggctcttgtc gcgcttcgcg tcgccgaaat
     4021 cagatgaggc gagaatggaa tcctt
//
LOCUS       DASTQY010000474        10521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1641395, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000474 DASTQY010000000
VERSION     DASTQY010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 10521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10521
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641395"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2366)
                     /gene="leuS"
                     /locus_tag="VFR82_15220"
     CDS             complement(<1..2366)
                     /gene="leuS"
                     /locus_tag="VFR82_15220"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU4502999.1"
                     /translation="MSKTYDHHAIEAKWQAYWEAHRPFRAVEDPAKPKFYCLDMFPYP
                     SGSGLHVGHLEGYTATDIISRYKRMKGFNVLHPMGWDAFGLPAEQYAVKTGIHPAITT
                     AQNIATFKRQMKRVGLSYDWDRELSTTDPGYYRWTQWIFLQLFKRGLAYVAEVPVNWC
                     PALGTVLANEEIVDGKSEVGGFDVIRKPMRQWVLKITAYADRLLDDLSLVQWPPSTLE
                     MQKNWIGRSIGAEVDFRIHDFNGAIRIFTTRPDTLFGATYIVLAPEHPLVEVVTIDTE
                     RGAVDAYRQAAARKSDLQRQELDKEKTGVFTGGYAINPVNQERLPIWIADYVLMGYGT
                     GAIMAVPAHDARDWAFAKTYHLPIREVIAGGDVEKEAFVETEKGTVVNSTSPDRRFSI
                     DGLAPVDAIPKITAWLESIGTGRKSVNYKLRDWLFARQRYWGEPFPVVWVDGEARPLP
                     EEQLPLALPETNNFTPSGNGESPLANLEHWLKTTDPDSGKPARRETNTMPQWAGSCWY
                     YLRFLDPKNRDQAVDPVKEQYWMPVDLYIGGSEHAVLHLLYSRFWHKVLFDIGVVSTP
                     EPFKKLVHQGIVLGEDNQKMSKSRGNVVNPDEMIDRFGADAVRLYEMFMGPLEAMKPW
                     STRGVEGVTRFLERVWRLMVDEQGGLSSAIVSSVPQVEQQRILHQTIKKVTEDIEHLR
                     FNTAISQMMVFTNEMTKLPQRSRTLIEPFLLLLSPVAPHLTEELWSIIGNRPSISQQP
                     WPSFDSALTMSDRLTIPVQVNGKLRGKLEVSTDADRQQVPELAREVAA"
     gene            2519..3094
                     /locus_tag="VFR82_15225"
     CDS             2519..3094
                     /locus_tag="VFR82_15225"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4503000.1"
                     /translation="MSSQVCQGLRGDLLKEAHGDVLEIGFGTGLNLPHYPATVSQLWM
                     VDPARFLLSRVQMRVAQAAFPVHMEQLAAESLPFPDQRFDSIVSTWTLCTIPDPLKAL
                     REIRRVLKPHGVFLFLEHGRSREARIAAWQDRFNPIQNLIACGCNLNRPIDLLIERSG
                     FHLTDLSRFQIPHVPRIAAEMYQGHARSTKC"
     gene            complement(3101..3760)
                     /locus_tag="VFR82_15230"
     CDS             complement(3101..3760)
                     /locus_tag="VFR82_15230"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU4503001.1"
                     /translation="MLNWDRIDDVLLDMDGTLLDRHFDNFFFEEELPRRYAAAQGLSF
                     EASRACLMAMYRSVEGQLAWTDLHYWSDRVGIDVVAMHKELDHMIGFLPGAEGFLKEL
                     RARGKRIIVVTNAHQAGVAVKSAKTGLGRYVDRIVDAFEVGYLKMRSEYWPACRRLLK
                     FDPLRSLYIDDDESCLHAARQYGIGSLIHSAKSSSQLPAVYSNDFASVERLSTLLNGT
                     L"
     gene            3845..5248
                     /gene="der"
                     /locus_tag="VFR82_15235"
     CDS             3845..5248
                     /gene="der"
                     /locus_tag="VFR82_15235"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU4503002.1"
                     /translation="MKNAVLRSITVSTAHPTPTPSVLSPQPTIVAPTVAIIGRPNVGK
                     STLFNRILGTKTAIVDDVPGVTRDRNYADANYRNRPFRLVDTGGLEPSASEGMLALIK
                     RQSELAIEEAEILILLVDGRTGLMPQDREVIRLLRGTTKPLFLAVNKIDTPKVEPLVA
                     DFYQLGIEQLFPVSAEHGIGVSELLDAIYPLLPAPEEQSGPPSMPRIAVVGRPNVGKS
                     TLVNAVLGEERVVVSDVPGTTRDAIDSLAIYDGRRYLFTDTAGIRRRGKVDRGIEGYS
                     VARSLRAIGRSDVAVLLLDAEEGVTEQDTKIAGVVIRQGRACVLLVNKWDRREADPLA
                     GQKFEKDLKRRFPFLSWAPVLFGAAVKPDSLRRLFPLIDEVFQSFSRRIPTGQLNQFL
                     QNLLAVHPLPVRKGKPAAITKSAFMTQVAVRPPVFALFVGHPDNITPAYLRFLENQIR
                     ETYGFAGTPLRLLVRKK"
     gene            5367..6242
                     /locus_tag="VFR82_15240"
     CDS             5367..6242
                     /locus_tag="VFR82_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503003.1"
                     /translation="MKTSRLIICGFRNPRRLLALWLLLFICSLAAGASPGALWAAEGG
                     QANDGDAPPQTTAPDSQPGVAPAEVDPSITLKGAVWRTKAGIVFLKTPVGLLTLSSKT
                     TLKDLKASHEVSFWVHDRHVAVEIRKRSDGSLVHRYLSGPMTLGPDSSKTLRCWTADG
                     EQTVHYGTQETKLAAYREGDPLTVEVDESQTIIGVHDLQFDLQISQTPPAGSPAHVLL
                     SGSVSKLKSNFVFFRTPVGVVMINAKIGIPPVKVGHTLTLHIDNGRVNVEVTKTAKPA
                     ARSNAGPATAPTTPF"
     gene            6289..6906
                     /locus_tag="VFR82_15245"
     CDS             6289..6906
                     /locus_tag="VFR82_15245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4503004.1"
                     /translation="MRPVAPFAKTPDAFDKLYRDHVDLVYRYAYRLCGDMESANDLVQ
                     ETFLNAFRGMNAFRGDARVSTWLYTIASRAAMRLRRKRKGAPERELSLEEFIPTSDGE
                     FRLQIPIEGLTPEQALENKELREALDQAITKLPKKYRIALVLRDMEGLSAKEVSGIMG
                     LSERAVKSRLHRARLFVRRELSGRGITEEHRGSDAKGWRPRRSGS"
     gene            6903..7214
                     /locus_tag="VFR82_15250"
     CDS             6903..7214
                     /locus_tag="VFR82_15250"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU4503005.1"
                     /translation="MTKKKPARRHSTSTASHPHQPGEGRCLDILRQLSAYIDDELAED
                     ICAELRRHVGACPNCEEFIASLRDTVTLCRHHQTPPLSSQDRAAIRHNILQAWKPEGP
                     R"
     gene            7211..7603
                     /locus_tag="VFR82_15255"
     CDS             7211..7603
                     /locus_tag="VFR82_15255"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU4503006.1"
                     /translation="MRQHVAITALLGGMLGVIGSSPATPVSPGDHGEGRTVYDRHCAE
                     CHGPEGRGDGPKATSLSPRPGNLISAQTSAKSDQDLLKIIANGRPRTAMAGWNDRLSR
                     DEQLAVLAYIRSLIRFTGSATPPPPSNP"
     gene            7709..9391
                     /locus_tag="VFR82_15260"
     CDS             7709..9391
                     /locus_tag="VFR82_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503007.1"
                     /translation="MKLIARETSTVVLVLFVGLFFAFPATTAAAKKSKGMAPGAATAL
                     QYADAISKGDIVKTANLDFACQYRLAAAKRSGLTSSSDKSDTSADSCWEGIKTAHNSV
                     LDRVDTGMDVLWPTNGALPFFRDDLTHYPASAFVMDLVGLSPPGSGLHLEAVSTTPLP
                     NASFHLRPQEPLISVPATLVTLTVTYQDPLTAPITKAPGSYKWTNTVKPARRAVKKLN
                     TQWVVLSGLKRHGFSGDAAVVNLLVQDGGPAEGIQQEIIPFVTEKSSAIPGSVVWWGP
                     EDAPGLLVASAARAAAFPNLRDRVALLNRILIIDPHQADALTVLTRDLYAVILREAIA
                     AHKLTVKDPSLAMAVNEQYWNVYAQGERLDLSLDMEMGGLDKPTTADYLYRLLPALRT
                     LAQVRPEQLDNRFRWGAALRWNLDQDLSIEVHEQLVKDVPAERKTAKAEALLQLAWSR
                     INKVAWNRNLDDKDVQAAYRDAEQAAALAEIPLDKFIAEYTKAYSLLFSPSRDNRAIL
                     GHLTEAKRWFDEIPGNNQQVWDFFIGADSLKSVLDADPIFQPLLASGASHKG"
     gene            9417..10106
                     /gene="aat"
                     /locus_tag="VFR82_15265"
     CDS             9417..10106
                     /gene="aat"
                     /locus_tag="VFR82_15265"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEU4503008.1"
                     /translation="MSRNPDDLTFPSVEQASSEGLLAVGGDLRFERLLEAYRRGIFPW
                     YSEDQPILWWSPDPRTVLYPEKLHISRSLKPSLRPGRFTVTLDTCFEEIVEHCAGPRP
                     QYPQGGTWITPAMREAYAALRQEGYAHSVETWQEGRLVGGLYGVAIGGAFFAESMFTR
                     VDDASKVALVFLIRQLRTWGFRLVDCQQSSPHVLRLGAEDIPRRLYIEQLAGAVQQRD
                     RRGRWQFDAAP"
     gene            complement(10110..>10521)
                     /locus_tag="VFR82_15270"
     CDS             complement(10110..>10521)
                     /locus_tag="VFR82_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEU4503009.1"
                     /translation="HGELKVSMRLVPDQTPEKVFALLKQYVAKLNPSVKVEREGMLHP
                     FKGSFEGPYVECVRRAAKAGFGHEPAFIREGGSIGAVVTMQKTWKVPILFLGLSLPEH
                     GYHAPNEYFDWGQASGGMKAFAHYFMELAKMGKR"
BASE COUNT         2197 a         3151 c         2966 g         2207 t
ORIGIN      
        1 tctgcggcca cttctcgggc caactccgga acctgctggc gatcggcatc ggtactcacc
       61 tcgagttttc cccgcagctt cccgttcacc tgaaccggga tggtcaaccg atcactcatc
      121 gtcagggcag aatcaaagga aggccacggc tgctgagaga tgctcgggcg gtttcctatg
      181 atcgaccata gttcttccgt cagatgggga gcgacggggg acagtagcag cagaaatggt
      241 tcaatcaagg tccgtgaccg ttgaggaagc ttggtcatct cattggtgaa gaccatcatc
      301 tgagagatgg ccgtgttgaa tcgaagatgc tcaatgtctt cggtcacctt cttgatcgtt
      361 tgatgaagga tccgctgttg ctcgacttgg gggacggacg acacgatcgc ggacgagagc
      421 ccgccttgct catcgaccat caaacgccag acccgctcga gaaagcgggt gacgccctct
      481 acgcctcgcg tgctccaggg cttcatggct tccaatggtc ccatgaacat ttcatagagt
      541 cggacggcgt cggccccgaa gcgatcgatc atctcgtcag ggttcacgac gttgccacgg
      601 gacttggaca tcttttgatt gtcctcaccc aagacgatgc cctggtggac gagcttcttg
      661 aacggttccg gtgtcgagac gacgccgata tcgaacagga ccttatgcca aaatcgggaa
      721 tacaggagat gcagcacggc atgctcgctg ccaccaatgt agaggtcaac cggcatccaa
      781 tattgttctt tcacagggtc gactgcctgg tctctgtttt tcggatcgag aaaccgcaaa
      841 taataccagc aggacccggc ccattgcggc atggtattgg tttcgcgccg cgccggcttc
      901 ccgctatccg ggtctgtcgt tttgagccaa tgctccagat tggccagcgg actctccccg
      961 ttgcctgacg gggtgaaatt gttcgtctca ggtagtgcca gcggcaattg ttcttccggg
     1021 aggggtctgg cttcgccatc cacccatacg acgggaaacg gctctcccca atagcgctgg
     1081 cgggcgaaca gccaatcgcg aagcttgtaa tttacggact tgcggcctgt cccaatggat
     1141 tcaagccatg cggtgatttt gggaatggca tcgaccggcg ccaggccgtc aatcgaaaag
     1201 cgtctgtctg gcgaagtcga attgacgacc gtccctttct cggtctcgac gaaggcctcc
     1261 ttctccacat ctccgcctgc aatgacttct cggatgggca agtgataggt cttcgcgaac
     1321 gcccagtcgc gcgcatcgtg cgccgggacg gccataatgg caccggtgcc gtagcccatc
     1381 aagacgtaat cagcgatcca gatgggaagc cgctcctgat tgaccggatt gatggcatag
     1441 cccccggtaa acactcccgt cttctctttg tccagctctt gccgctgtaa atcgcttttg
     1501 cgggcggcgg cctggcgata ggcgtcgacg gccccacgct ctgtgtcgat cgtcacgacc
     1561 tcgacaagcg gatgctccgg ggcgaggacg atatatgttg caccaaacaa cgtatcaggc
     1621 ctcgtcgtga agatcctgat cgctccgttg aagtcgtgaa ttctgaaatc tacttccgcg
     1681 ccaatcgaac gcccgatcca gttcttttgc atctccaagg tgcttggcgg ccactgcacc
     1741 agcgacaaat cgtcgagcaa ccggtccgca taggccgtga ttttgagtac ccactggcgc
     1801 atgggtttgc ggattacgtc gaagccgcca acttcactct tgccgtcgac gatctcttca
     1861 ttggccaaca cagttcccag cgccggacac caattgacgg gaacttctgc gacgtaggcc
     1921 aagccccgct tgaacaattg cagaaagatc cattgcgtcc agcggtaata gccggggtcc
     1981 gtcgtactca gttcgcggtc ccagtcgtat gacaatccga cgcgcttcat ttgccgtttg
     2041 aacgtggcaa tattttgcgc cgtcgtgatg gccggatgga tgccggtctt caccgcatac
     2101 tgttcagcag gcaatccgaa ggcgtcccag cccatgggat gcagcacgtt gaagcccttc
     2161 atccgtttgt accgggagat gatatcggtc gccgtatagc cttcgagatg gccgacatga
     2221 aggcccgacc cggagggata gggaaacatg tcaaggcagt agaattttgg cttggccgga
     2281 tcttcaaccg ccctgaacgg gcgatgcgct tcccagtagg cctgccactt cgcttcgatg
     2341 gcgtgatgat cgtaggtctt actcatgcgg tcgggatcgg tcggaagagg aagaactcta
     2401 acatagactt ctcgaaggca acaacaatcg ccgggcccca tcaacggggc tttgctatac
     2461 tgcatccctc atggggttgt acagcgagca cgtattcccc cgcctcatgg actgggtcat
     2521 gagcagccag gtgtgtcagg gcctgcgggg cgatctgcta aaggaggctc acggcgatgt
     2581 cttggaaatc ggattcggca ccgggctgaa cctgccccac tacccggcga cggtttcgca
     2641 actgtggatg gtggaccctg cgcgcttcct actgagcagg gtgcaaatgc gagtcgcgca
     2701 agcggccttt cctgttcaca tggagcagct cgctgcggaa tcactcccgt ttccagacca
     2761 gcgattcgat tctatcgtca gtacgtggac gctctgcacc attcccgatc cattgaaagc
     2821 gcttcgcgag atcagaaggg tactcaaacc acacggcgtt tttctctttc ttgagcatgg
     2881 acgcagccgg gaggcgcgca tcgccgcctg gcaggatcgt ttcaatccga tccaaaacct
     2941 cattgcatgc ggctgtaacc tgaaccgtcc gatcgatctg ctcatcgagc gaagcggatt
     3001 tcacctcacg gacctcagcc gctttcagat accgcatgtg ccacgcatcg ccgcagagat
     3061 gtatcaaggc cacgcacgca gtaccaagtg ctgagcacga ttacagtgtg ccgttcagaa
     3121 gcgtcgagag ccgctcgacg gatgcaaaat cgttagaata gacggcgggt aattgcgaac
     3181 tggattttgc gctgtggatg agggacccga tgccatattg cctggctgca tgaaggcaac
     3241 tctcgtcgtc atcaatatag agagaccgca gtggatcaaa tttcagcagc cgtcggcacg
     3301 ccggccagta ttccgatcgc atcttgaggt accccacttc gaacgcatcc acgatccgat
     3361 ccacatatcg gcccagaccg gtcttggccg atttgaccgc cacaccggcc tgatgggcat
     3421 tcgtcaccac aataatccgt ttgcctcttg cccggagctc ctttaagaag ccttcggctc
     3481 ccggcaaaaa accgatcatg tggtccagtt ccttgtgcat cgccacaaca tcgatgccga
     3541 cccggtcgct ccagtaatgc aagtccgtcc aggcgagttg gccttcaact gagcgataca
     3601 tcgccatcaa gcaagcgcga gaagcttcga aagagagccc ctgcgcggcc gcatagcggc
     3661 ggggcagctc ttcctcaaaa aagaaattgt cgaagtggcg atcgagcaac gtgccgtcca
     3721 tgtccagaag cacgtcgtcg atccggtccc aattcagcat gtgagagggc cggattctga
     3781 ttgttgatga atcatgagca gagtatatcg aagcgccgaa aggaagacga gacgggttgt
     3841 ctttgtgaaa aatgctgtgc tacggtcgat caccgtgtcc actgctcatc caacacccac
     3901 gccttcagtc ctcagccctc agccgacgat cgtggctccc acagtcgcca tcatcggacg
     3961 gccgaacgtg ggcaaatcca cgttgttcaa ccggattctt gggacaaaaa cagcgatcgt
     4021 cgacgatgtg ccgggggtga cacgcgaccg gaactatgcc gatgcgaact accgaaaccg
     4081 cccctttcgg cttgtcgaca cgggaggcct tgaaccgtcg gcatcggaag gcatgctggc
     4141 gctgatcaaa cgtcagtccg aactggccat cgaggaagcg gagattctga tcctgctcgt
     4201 ggacgggcga accggattga tgcctcagga ccgtgaggtc atccgtctcc tgcgtgggac
     4261 gacaaagccg ctgttcctcg ccgtcaacaa gatcgatacc ccaaaggtgg agccgttggt
     4321 cgcggatttt tatcaactgg gaatcgagca actgttcccc gtctcagcgg agcatggcat
     4381 cggagtctcc gaactgctcg atgccattta cccgctgctc ccggccccgg aagagcaatc
     4441 aggaccgcct agcatgcctc gcatcgccgt ggtcggacgg ccgaacgtcg gcaagtccac
     4501 cctggtcaat gccgtcctcg gcgaggagcg ggtggtcgtc agcgacgtac cagggacgac
     4561 gagggatgcc atcgattccc tggctatcta tgacggccgt cgttatttgt tcaccgacac
     4621 ggccggcatc aggcgccgtg ggaaagtcga tcgtgggatc gaaggctaca gtgtcgcgcg
     4681 ttcgctccgg gcaatcggac ggtcagacgt tgccgtcctc ttgctcgatg ccgaagaagg
     4741 ggtgacggaa caagacacca agatcgccgg cgtagtgata cgccaggggc gtgcctgcgt
     4801 gttgttggtc aacaagtggg accggcgcga ggccgacccg ctcgccggac agaaatttga
     4861 aaaagactta aaacggcgct ttccgttcct atcgtgggcg cccgtgctct tcggagccgc
     4921 ggtaaaacct gattctctcc gccgcctatt tccgctcatc gatgaggtct ttcaatcgtt
     4981 ttccaggcgc attcccaccg gtcagctcaa tcagttcctg caaaatctct tggccgttca
     5041 tccactaccg gtgcggaaag gaaaaccggc ggcaatcacg aaatctgcat tcatgactca
     5101 agtggcggtc cgacctcctg tctttgcgct cttcgtcggc catccggaca atatcacgcc
     5161 ggcctatctc cggttccttg aaaaccaaat ccgtgagacg tacggattcg caggcacgcc
     5221 ccttcgcctg ctcgtcagaa aaaaatagca gggctattgc atcagagtca tgcacagccc
     5281 ccaccaacga cagagagcgc tccggtgggc tttcagcttg actctacgac atcatcgtgg
     5341 ttaattcgcg cggtcacttg tcgtcaatga agacttcacg gcttatcatc tgcggtttcc
     5401 gcaatccccg gcggctgttg gcgctttggc tgctgctttt catttgcagc ctggcggccg
     5461 gcgcttcgcc cggtgccctt tgggctgccg aggggggtca ggccaatgac ggtgacgcac
     5521 ctccccagac gactgcaccc gactctcaac cgggcgttgc gcctgcggag gtggaccctt
     5581 ccatcacatt gaagggcgcg gtgtggcgga caaaggcggg aatcgtcttc ttgaaaacac
     5641 cggtgggact gctgacactc agctctaaga cgacgttgaa agacttgaaa gcctcgcacg
     5701 aggtctcgtt ctgggtgcat gaccgccacg tcgccgtcga gattcgcaaa cgaagcgacg
     5761 gatcgctcgt tcaccggtac ctcagtgggc cgatgacact tggcccggat tcatccaaga
     5821 ccctgcgctg ttggaccgcc gacggcgaac agacagtcca ttatggcacc caagaaacca
     5881 agctcgcggc atatcgtgaa ggcgatccat tgacagtcga agtggatgag tcacagacga
     5941 tcattggcgt ccatgatctg caattcgatc tgcagatcag ccagactcct cccgccgggt
     6001 ctcccgccca tgttctcttg tccggttcag tctcaaaact gaaatcgaac ttcgtattct
     6061 tcaggacacc ggtcggcgtc gtgatgatca acgccaagat cgggataccg cctgtgaagg
     6121 tcggacacac gctgactttg catattgaca acgggcgtgt gaacgtcgag gtgacaaaga
     6181 cagccaagcc tgcggcgcgc agcaacgcag ggccggcgac agctccgacc acgccgttct
     6241 gagccctgtt tgacagtctc cagttcgact ccctagactc ccctgaccat gcgcccggtc
     6301 gctccatttg caaaaacacc tgatgcgttt gataagctgt atcgagacca tgtcgatctc
     6361 gtttatcgct acgcctatcg actttgcgga gacatggagt cggcgaacga cctcgtccaa
     6421 gagacctttt tgaatgcctt tcgcggcatg aacgcgtttc gtggcgacgc tcgagtttcc
     6481 acgtggctgt ataccattgc gtcgcgagca gctatgcgcc tgcgtcgcaa gcggaaaggg
     6541 gcgcctgagc gggagctttc gctggaagaa ttcatcccca cctctgacgg tgaattccgc
     6601 ttgcagattc ccatcgaggg ccttacgccg gagcaggcgc tcgaaaacaa ggaattgcgc
     6661 gaggcgctcg atcaagccat caccaagctg cccaagaagt accggatcgc gctggtgctg
     6721 cgggatatgg aagggttgag cgcgaaagag gtctcaggga ttatggggtt gagcgaacga
     6781 gcggtcaaat cccggctgca tcgagcacgg ctgtttgtga ggcgggagct gagcggtagg
     6841 ggaattactg aagagcatcg agggtcggac gctaaagggt ggcggccacg aaggagcggc
     6901 tcatgaccaa gaagaaaccg gctcgtcgac acagcacttc gactgcctct catccccatc
     6961 agccgggaga gggccggtgt ctcgacattc tccgacaatt atccgcatac atcgacgacg
     7021 aattggctga ggatatttgc gccgagctgc ggcggcatgt cggagcctgc cccaattgtg
     7081 aggagttcat agcctcgctg cgcgacaccg tgacattgtg ccgacatcat caaacgccgc
     7141 cgctctcctc acaggaccga gccgcgatcc gccacaatat cctccaagcc tggaagcccg
     7201 agggacccag gtgagacaac acgttgcgat cacagccttg ctcggcggga tgctcggcgt
     7261 catcggatca tctccggcaa cccctgtttc accgggcgat cacggggaag ggcgcacggt
     7321 ctacgatcgc cattgcgccg agtgccacgg tccggaaggc cgaggtgatg ggccgaaggc
     7381 gacatctctc tcgcctcgtc ccggcaattt gatttctgca cagacctccg caaagtccga
     7441 tcaagatttg ctcaagatca tcgccaatgg ccgacctcgc accgccatgg ctgggtggaa
     7501 tgatcggttg tctcgcgacg aacagctggc cgtgcttgcc tatatccgat cgctcattag
     7561 atttaccggc tcggccacgc cccctccgcc gtcaaaccca taaccgttgc atgtatggtg
     7621 ctgagcggtc gacccctttc agacgcgaaa atccgcatgg tacagtatcg gctttcgcct
     7681 ctttgctttc gtaaggagta aacctggcat gaagctgatt gcgagagaaa catccacggt
     7741 cgtgctcgtc ttgtttgtcg gactcttttt cgccttcccc gctacgactg cagctgcaaa
     7801 aaaatcgaaa gggatggcac ctggtgcggc gacggccctt cagtacgccg acgccatctc
     7861 gaagggcgac atcgtgaaga cggcgaacct cgactttgct tgccaatatc ggctcgccgc
     7921 cgcaaagagg tccggtctca cctcttcttc ggataagtct gatacttccg ctgatagctg
     7981 ctgggagggc atcaaaactg ctcacaactc tgtcctcgac cgagtggaca ccggcatgga
     8041 cgtcctctgg cccaccaacg gcgcgctgcc gttttttcgc gatgatctga cgcattaccc
     8101 ggcgtccgcc tttgtgatgg atcttgttgg cctgtcccct ccaggcagcg gtctgcatct
     8161 tgaagcggta tccacgacac ctttgcccaa tgcgtcgttt catttacggc cgcaggaacc
     8221 gctcatctcg gtgccggcaa cactcgtcac gctcacggtg acctatcagg atcccctgac
     8281 cgcgcccatc acgaaggccc cgggtagcta caaatggaca aacaccgtca agcctgcgcg
     8341 gcgggccgtc aagaaactca acactcaatg ggtggtgctc tcaggattga agagacacgg
     8401 attctccggt gatgcggcgg tcgtcaatct tctggtgcag gatggcggtc ctgccgaagg
     8461 catccagcaa gagatcattc cgttcgtgac ggaaaagagc agtgcgatcc ccgggtcggt
     8521 cgtctggtgg ggacctgagg atgcacccgg cctcctggtc gcctctgcgg cacgcgccgc
     8581 agcgtttcca aatttgcgcg accgcgtcgc actcttaaac cggattctca tcatcgatcc
     8641 gcaccaagcc gacgcgctga cggtcctcac acgtgacctc tatgccgtca ttctgcggga
     8701 agcgattgct gcacacaaac tcaccgtgaa agatccgtct ctggccatgg cggtcaatga
     8761 acaatattgg aatgtctatg cccaggggga gcggctcgac ctctccctgg atatggaaat
     8821 ggggggactg gacaagccga ccaccgcgga ctatctctat cgcttgcttc ctgcgctgag
     8881 gaccctggcc caggtccggc cggaacagct ggacaatcga ttcagatggg gcgcagccct
     8941 ccggtggaac ctcgatcagg atctctccat cgaggttcac gagcagttgg tcaaggacgt
     9001 gccggcggag cggaagaccg cgaaagcgga ggcgctgctc caattggcct ggtcacgcat
     9061 caacaaggtg gcatggaatc gcaaccttga tgacaaggac gtccaggcgg cctaccggga
     9121 tgccgagcag gccgctgcat tggccgagat accgctggat aagttcatcg ccgagtacac
     9181 gaaggcctat agcctgctgt tttcgccgag ccgagacaat cgcgccatcc ttggacacct
     9241 cacggaagcg aaacgatggt tcgatgagat cccgggcaac aaccagcagg tctgggattt
     9301 tttcatcgga gcggactcgt tgaaatccgt tctcgacgct gatccgatct ttcaaccact
     9361 cctggcctca ggagcatctc acaaaggata actggcactg ctcagccatg gtgaggttga
     9421 gtcgaaatcc tgacgacctt acctttccgt ccgttgaaca ggcctcctca gagggcttgc
     9481 tcgccgtcgg gggcgatctc cgattcgaac ggctcctcga agcctaccgc cgaggaatct
     9541 ttccctggta cagcgaagat cagccgattc tttggtggtc ccccgaccca agaaccgtcc
     9601 tgtatccgga gaagctccac atttcacgaa gcctgaagcc cagccttcgc cccgggcgct
     9661 tcacggtgac acttgatacc tgttttgaag aaatcgtcga gcattgtgcc ggcccgcgac
     9721 cgcagtatcc ccagggtgga acgtggatca ctccggcgat gagggaggct tacgcggcgc
     9781 tccgccagga aggctatgcc cattcggtcg aaacctggca agaagggcgc ctggtcggag
     9841 gcctgtatgg agtggcaatc ggaggggcat tcttcgcaga atctatgttc acccgggtcg
     9901 acgatgcatc gaaagttgcc ctggtattcc tgatccgtca gctgcgtacc tggggctttc
     9961 gcctcgtcga ttgccaacaa tcctccccgc atgtgctgcg tctaggggcc gaagacattc
    10021 ctcgccgact gtacatcgag cagcttgccg gcgccgtgca gcaacgcgat cggcgcggcc
    10081 gttggcagtt cgatgctgca ccatgattgt tacctctttc ccatcttggc aagctccata
    10141 aaataatgtg caaaggcttt cataccgcct gaagcctggc cccagtcaaa atattcgttc
    10201 ggcgcgtgat agccgtgttc cggcaggctg agtccgagga agagaatagg cactttccag
    10261 gtcttctgca tcgtgacgac ggcaccgatg gaaccccctt cacggatgaa cgccggttcg
    10321 tggccgaacc ccgccttagc ggcacgcctc acacattcga cgtacggtcc ctcgaagctg
    10381 cccttgaagg gatgcagcat gccttcccgt tcgactttca cgctgggatt gagctttgcc
    10441 acatactgtt tgagcagagc aaagaccttt tcaggcgtct gatcgggtac gagacgcatg
    10501 ctgaccttga gctcaccatg t
//
LOCUS       DASTQY010000475         5718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1531199, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000475 DASTQY010000000
VERSION     DASTQY010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531199"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..627
                     /gene="sugE"
                     /locus_tag="VFR82_15275"
     CDS             307..627
                     /gene="sugE"
                     /locus_tag="VFR82_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEU4503010.1"
                     /translation="MAWVILFLAGLFETGWAIGLKYTEGFTRLWPTVWTVFAMVVSLW
                     LLGIAMRTLPVGTAYSVWVGVGAVGTVALGIALLEEPVNAARLASVGLIIAGIIGLKL
                     ATPA"
     gene            complement(692..967)
                     /locus_tag="VFR82_15280"
     CDS             complement(692..967)
                     /locus_tag="VFR82_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503011.1"
                     /translation="MRKMFVMTLVGLAIVGGAGWSVLAGETKARMEDSKGEMKADFEE
                     AKGGAKALKEEVKGNDTKADVERAKGNVKGAGERVKGKSKAAKERAK"
     gene            complement(1069..2604)
                     /locus_tag="VFR82_15285"
     CDS             complement(1069..2604)
                     /locus_tag="VFR82_15285"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF002868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEU4503012.1"
                     /translation="MELARLVDAVERVRATTKKSEKARVLADILRQTSGHETKLAALY
                     LTGSLPQGKIGIGWHLIQKAIQNVPVSGLPLTLSDVDKGLTLLAAERGGGSSERRVVE
                     LSRLTSRARSEERRFLSQLLLGELRQGALEGVLLEAIAIAAGVPPTEVRQASMFAPNI
                     GDLAQVALAEGSGGLARYSLRLFVPVAPMLANSAEDIEAALERHNPAAFEYKLDGARI
                     QVHKGGTEVRIFTRQLQDVTERLPEIVQWVQTLPAQEAVLDGEAIGLRQDGRPQPFQV
                     TMRRLGRVKDIAAIRDAIPLTPFLFDALYVDGDSLLSHTYEERTGVLTSVAPGATVQR
                     LLTAQPEEARRFLAQALAAGHEGVMAKSLSAPYIAGQRGFHWIKIKEATTLDLVILAA
                     EWGNGRREGWLSNLHLGARDPASGGFVMLGKTFKGLTDEMLRLQTEKLLALETHREGH
                     TVFVRPEIVAEIAFSDVQESPRYPAGLALRFARVKRYRPDKSPQEADTIQTVSGLFQT
                     QRA"
     gene            3228..5411
                     /locus_tag="VFR82_15290"
     CDS             3228..5411
                     /locus_tag="VFR82_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEU4503013.1"
                     /translation="MSTNSPPTSRPQSPARSAPGLHADTGLFSLLILARFYDLPADAS
                     QLRLLFVSSAAHFSDIELLRAAKHVGLKAGVVKSRWDSLSMTPFPAIAKQRDGRYVVL
                     AKVHGEKILIQDPVESRSLVLSREQFESNWTGELLLFAKRANVRQQDLRFDFTWFIPA
                     IVKYRKFLGEVFVASFVLQLFALLTPLFTQVLIDKVLVHKGFTTLHVLAIGMVTMAIF
                     DAALGGLRTYLFAHTTNRIDVGLGAQLFRHVLALPLAYFEARRVGDTVARVRELEHIR
                     QFLTSHSVTVVLDVVFTLVFLIVMWFYSHTLTMVVMASLPLYAVLSIAITPTIRARLH
                     EKFNRGAENQSFLVEAISGIQTVKALAVEPPLIRKWEEQLAGYVRASFRSTSLITVAS
                     QIAGCIQKLTTVAVLWVGAYRVIDGDLRIGQLIAFNMLSAQVTGPLLRLVNLWQEFQQ
                     VGISVQRLGDVLNTQPEPSYKPNRTTLPQVAGQVSFEDVTFRYRPDSSEVLRKISFSV
                     AAGKVIGIVGRSGSGKSTIAKLIQRLYVPERGRIVVDNVDLAQVDPAWLRRQVGVVLQ
                     ENFLFNSSVRDNIALADPGLGMEQVIQSAQLAGAHEFILELPDGYDTIVGEHGCSLSG
                     GQRQRIAIARALVANPRILIFDEATSALDYESEAVIRKNMVQICQGRTVFIIAHRLST
                     VRPADQIYVVEKGEIVEKGSHEELLRTNGFYTRLCTLQDTAGARA"
     gene            5429..>5718
                     /locus_tag="VFR82_15295"
     CDS             5429..>5718
                     /locus_tag="VFR82_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503014.1"
                     /translation="MAFSSISRYWIIWKAAWRAQSTRQLGMASGWRTTEFLPAVLEIE
                     QTPPSPIGRAISWTIIGVFISGVLWATVGWIDIVATAQGKIIASGYSKVIQP"
BASE COUNT         1174 a         1591 c         1655 g         1298 t
ORIGIN      
        1 aggcattgac cctctgatcc gtcttaatga gcccgtcctc tgtccaccag gatcggccgg
       61 aggtcgacga caagaaattg ctaatcgtct cggtcacgct gcaggccgag agagacaata
      121 tgagtggggc acaagcgacg aggatccacc tgtatcctgg gtgtgtcatt gcaaccctcc
      181 tacgatggta agtgatcgtt ggatatctgc ctcctcttca ctctagagca gaacgaatag
      241 aggatcaagc catgtgcagt cgacagcagt tgaacaggtt gagtgatcac agaggagaga
      301 aattctatgg catgggttat tttgtttctc gcggggttgt ttgaaacggg gtgggcgata
      361 ggactgaaat acacggaggg gttcactcgc ttgtggccca ccgtgtggac tgtgttcgcc
      421 atggtcgtta gtctttggct actaggaatt gccatgagga cgctccctgt cggcacggca
      481 tacagcgtat gggtcggagt gggagccgtg ggaaccgtcg cgttggggat tgcattgctg
      541 gaagagccag tcaatgcggc gcgcttagcc agcgtcggtc tgatcattgc cggcattatt
      601 ggcctgaagc tcgcgacacc tgcctaatct tgtcccggcg agtccacaag tcggccgatg
      661 ctagcggcgt cgaggtcttg ctagcgatag actacttggc tcgttccttc gctgctttcg
      721 acttgccctt cacccgttcg ccggcgccct tcacattgcc tttagcgcgt tccacgtcag
      781 ccttcgtgtc attgcctttc acttcttcct tcagagcctt ggcccctccc ttggcttctt
      841 caaaatccgc cttcatctcg cccttgctat cctccatccg cgccttggtc tcgcccgcca
      901 gaaccgacca gcctgccccg cccacaatag ccaggccaac cagggtcatc acaaacatct
      961 tgcgcatagt acctcctagg agagaacagt ctcctatctt ggttctagcg ttactactac
     1021 atacgtccta ctagagtgtt ccctaggaag aagactgggg atgtatggtc aagctcgttg
     1081 ggtttgaaag agtccgctga ccgtctgaat cgtgtctgcc tcttgcgggg acttgtctgg
     1141 acggtagcgt ttgactcggg cgaagcgaag agcaagaccg gctgggtagc gtggcgactc
     1201 ctgtacgtcg ctaaaggcga tctctgcgac aatttcaggg cggacgaaca ccgtatgtcc
     1261 ttcccggtgg gtttcgagag cgagcagttt ttcagtctgc aggcgcaaca tctcatcggt
     1321 aaggcctttg aaggtcttgc caagcatgac gaagccgccc gatgcgggat cacgggcacc
     1381 gagatgcaga ttcgacaacc acccctctcg acgaccattg ccccactcgg cggcaagaat
     1441 gaccaaatcc aaggtggtgg cttccttgat ttttatccaa tgaaaccccc gctggccggc
     1501 aatgtatgga gcggacagtg acttagccat tacgccctcg tgaccggcgg cgagggcttg
     1561 agccaaaaaa cgccgggctt cttccggttg cgccgtcagc agacgttgga cagtcgcccc
     1621 aggggctacc gatgtcaata ccccggtgcg ctcttcatac gtgtgtgaca ggagtgaatc
     1681 gccatcaacg tacagagcat cgaacaagaa cggtgtcaaa ggaatcgcat cgcgtatcgc
     1741 agcgatgtct ttcacgcgac cgagccggcg catggtcact tggaatggct gtggtctgcc
     1801 gtcctggcgc agtccgatcg cctcgccgtc tagcactgct tcctgggccg ggagtgtttg
     1861 tacccactgc actatttccg gcagtcgctc ggtcacatcc tggagctgtc gggtaaaaat
     1921 gcgcacctcg gttccgccct tgtgcacctg aatgcgtgca ccgtccagct tgtattcaaa
     1981 tgcggcagga ttgtgacgtt ccaatgccgc ctcgatgtcc tccgcgctgt tcgccagcat
     2041 cggcgcgacg ggaacaaaca gtcgaagcga atatcgcgcg agtcctcccg atccttctgc
     2101 gagggctact tgcgcgagat cgccgatgtt cggagcgaac atggaggcct gtcgcacctc
     2161 agtaggcggc actccagccg ctatggcgat cgcttccagc agcacccctt cgagcgcgcc
     2221 ctgcctaagt tcaccgagga ggagttggct gagaaaccgg cgttcttctg atcgcgcgcg
     2281 gctggtcaac cggctgagtt cgacaacgcg acgttcggaa gaacccccgc cacgctctgc
     2341 tgccagaagg gtcaaaccct tgtccacatc gctcaaggtc agcggcaggc cgctgacggg
     2401 gacattctgt attgcctttt gaatgaggtg ccagccgatg ccgatcttgc cttggggcaa
     2461 agacccggtg agataaagcg ccgcaagctt ggtttcatgg ccgcttgtct gccggaggat
     2521 gtccgccagc acacgcgcct tttcggattt tttcgtggtg gcacgaacgc gttcaacggc
     2581 atcgacaagg cgggccagtt ccatgctcca ttataaagga agggcggcgc gtgatgaagg
     2641 aaagagacgt cacggaagtc cgactatatt ggcagaggtc atggtcagtc gcggattcgt
     2701 agaggcaccg ccatccgctt gtctggaaga tgccatgagc aagatatgac aattacaacc
     2761 taggataaaa cgctcaagca aatcagttga caggaagccg aagcattcgt tacagtccca
     2821 cccctcgtaa aagttaggaa agcgtaaata atggtatttg cgttgtcatc gctcttctgg
     2881 ctcctcaaac aatgtcgctt cccaataaaa aaaggaaaca atgaattgag tcgagccaaa
     2941 ggccaagtcc tcaacgtgcg tctgaaaact catgactcat cggctcatcc gcgtgcggtc
     3001 acacgaacgt cgttgtttcg caaggcatcg cctatgtgga ttccgggttc attgagcccg
     3061 ccttgcacag aacgggcgat gggcatcgat gtcttggctc ggctccgtca acaagtatgg
     3121 aagcatgaat cagcttgccg catgcccggc ggccctgacc gggcttcagg ggacgatgag
     3181 gagaagaccg tccaggtcgc gagctggccc taggtgatct tgttacgatg agtacgaatt
     3241 cgccgcctac aagccgtcct caatcaccag cccggtcagc tcccggcttg catgcagaca
     3301 cggggctgtt ctcgctgctt attctggcgc ggttttatga tttgcccgct gatgcgtcgc
     3361 aattgcgcct gttgttcgta tcttccgcag ctcacttttc tgacattgaa ttgctccgtg
     3421 ctgccaagca tgtgggactc aaggccggag tagtgaagag tcggtgggac tccctgtcga
     3481 tgacgccgtt tcctgcgatc gccaagcaac gcgatggacg atatgttgtg ctcgccaaag
     3541 tgcacggcga gaagatcctg attcaggacc cggtcgaaag ccgttcgctc gttctctccc
     3601 gcgaacagtt cgagtcaaat tggacgggcg aactcctgct gtttgcaaag cgtgcgaacg
     3661 tacgtcagca ggatttgcgg ttcgacttca cctggtttat tcctgccatc gtcaaatatc
     3721 gaaaatttct tggcgaagtc ttcgtcgcct cgttcgtgct acagctattt gccctattga
     3781 cgcccttgtt cacccaggtg ctgatcgaca aggtgctcgt gcacaagggg tttacgactc
     3841 tgcatgtgtt ggccattggc atggtcacga tggctatctt cgatgccgcg ctgggcggtc
     3901 tccgcaccta tctctttgcg catacgacca accggatcga tgtcgggttg ggtgctcaat
     3961 tattccgtca cgtcctcgcc cttccactgg cctacttcga agcacggcgt gtcggggaca
     4021 ctgtggcgcg cgtacgcgag ctggagcaca tccggcaatt tctgacgagc cattcggtga
     4081 cggtcgtact ggatgtagtc ttcaccctgg tgttcctgat cgtcatgtgg ttttacagcc
     4141 acacactgac gatggtcgtc atggcgtcgt tgccgttgta tgccgtcctg tcaatcgcca
     4201 tcacgccgac gattcgtgcc agactgcatg aaaagttcaa ccggggcgcg gagaaccagt
     4261 cttttttggt cgaggcgatc agcgggattc agaccgtcaa ggcgttggcg gttgagcctc
     4321 cgctgatacg caagtgggaa gagcagctgg caggctatgt gcgggccagt ttccgctcga
     4381 cgagtctcat caccgtggcc agtcaaattg ctggctgcat tcaaaaactc acgaccgtcg
     4441 cggtgttgtg ggtgggggcc tatcgggtga tcgacgggga tctccgcata gggcagctga
     4501 ttgcgttcaa tatgctgtcg gcgcaggtga caggaccgct gctgcgtctg gtcaatctgt
     4561 ggcaggagtt ccaacaagtc ggcatttcgg tgcagcggtt gggtgacgtg ctcaatacgc
     4621 aaccggagcc gtcatataag ccgaatcgga cgacattgcc ccaggttgcg ggacaggtga
     4681 gcttcgaaga cgtgacgttt cgctaccggc ctgacagctc agaggtgctc agaaagattt
     4741 cgttctcggt cgccgctgga aaggtgatcg gcattgtggg tcgctctggg tccggcaaaa
     4801 gcacgatcgc caagctcatc caacggctgt atgtgccaga gcggggccgc atcgtcgtgg
     4861 ataatgtcga tcttgcgcaa gtggatcctg cctggctacg gcgacaggtg ggagtggtcc
     4921 tccaagaaaa ttttctcttc aacagttccg tgcgagataa catcgccttg gcggacccag
     4981 ggctgggaat ggagcaagtc atccaatcgg cgcaattggc cggcgcgcac gagtttattc
     5041 tcgaattgcc ggatggctat gacaccatcg tcggcgagca tggttgttcg ctctcgggcg
     5101 ggcagcggca acggattgcc attgccagag cattggttgc gaaccctcgt attttgattt
     5161 tcgatgaggc aaccagcgcg ttggattacg aatccgaagc cgtcatccga aagaacatgg
     5221 tgcagatttg tcaaggacgc acggtcttca ttatcgcaca ccggctcagc acagtccggc
     5281 cagccgatca aatctatgtc gtggaaaagg gcgaaatcgt cgaaaaaggg tcgcacgaag
     5341 aactcctgcg aacgaacggg ttttatacgc gcttgtgcac gctgcaagac accgcgggcg
     5401 ccagggctta gacggaaaat gtagagttat ggcattcagc tcgatctcac gatactggat
     5461 catttggaag gcggcctggc gggcccaatc gacccggcaa ctcgggatgg caagcggctg
     5521 gcggaccacg gaattcctgc cggcggtgct cgagatcgag cagacaccac cctcgcccat
     5581 tggtcgcgcc atcagttgga cgatcatcgg cgtgtttatc tctggagtgt tgtgggccac
     5641 cgtaggatgg atagatatcg ttgcgaccgc ccagggaaag atcattgcca gcgggtattc
     5701 caaagtcatt cagcccta
//
LOCUS       DASTQY010000476         4182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_515842, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000476 DASTQY010000000
VERSION     DASTQY010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515842"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1826
                     /locus_tag="VFR82_15300"
     CDS             141..1826
                     /locus_tag="VFR82_15300"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4503015.1"
                     /translation="MNRADRRRQDASREPRHQFTVQAWLDQGRVHQQAGRLIEAEDAY
                     RHALELAPHDAESSHLLGLVTYRLNRLAESLRYLHAAVERQPSSPVYWFNLGVVSQKA
                     GHSPDAVSAYEKAIALNPRYPEAYGNLGNVLRETGDLPGAVNAYEHALKLNPSHADTH
                     NNLGVAKKEQGQTRDAVACYRRALELRPTHAEALNNLGLALMEIGTLHDAIGCFQQAL
                     SILPGYQLARYNLGMAWSWAGDEIQACRCLHEVARARHDHRSVTADTSIYRSRIKHDT
                     EQVQFLFEQGKLGEDHRPYAQALRQLHERLESDSSPGNRLMVAPSELIAIAPSFNRIL
                     NCEPPPRIDHGALNPLLDVAAVEAQYHATRPEVTYIDNLLSQAALDALRRFCLEATIW
                     KKDYENGYSGAFLGDGFASPLLFQIADELRRKFPRIFSVHRMTQAWAFKHDSARRGLN
                     IHADAAAVNVNFWITPDEANLNPASGGLVVYDKEAPLEWNFKEYNSDRNKPKILTWLK
                     EAGAQAIKIPYRSNRAVVFNSDLFHETDDLSFKDDYLSRRINITLLYGYRSKS"
     gene            complement(1827..3956)
                     /locus_tag="VFR82_15305"
     CDS             complement(1827..3956)
                     /locus_tag="VFR82_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU4503016.1"
                     /translation="MDNSEKPAMVLVADDDNSVRQFVRAAIEQIGLEVCEASNGREVL
                     EQFTLRHPDVIVLDVMMPVMDGFAACSKLRGEAGGGKIPILMMTGLDDTDAIAMAYEH
                     GATDFIAKPLNATILSQRIRYLLRGSQTLRALLRSEARLGLAQRIAKIGNWEWQPESG
                     GFSASYELCRLMGIRPQDFGGTLDAYLQAVDIEDRERFHHALTQILTERTPCDIDHRI
                     VLPNGSEFTVNLQAEAVFDDQLKALTIVGTAQDISERKRSEREIHRLAYFDSLTGLPN
                     RVLFKDRVTQAIAHARRYQYNLALLFLDLDRFKITNDTLGHHIGDLLLKHVADRLMDS
                     VRHSDTIGRTTDTDRNHELARLGGDEFTVLLTNLRDVQDAGKVARRILEALARPFSIS
                     GHEVFVTVSIGIAIFPTDGDSVDLLLKSSDTAMYHAKEQGRNNFQYYSNAMNAAANER
                     LMLEAEVRQATEREEFVLYYQPQVDLRSGRIVGAEVLIRWLHPQRGLLGPNEFLQAAS
                     DTGIIRAIDEWVLRTACRQARAWQQRGLTVPSVSVNVSNSLFHGSTLLRAVQAALEET
                     GLTANRLELELTEPIAMRNLEASLTILHQLKNLGVQLSIDDFGTGHSSLSYLQRLPFN
                     RVKIDHSLIREILIKTEPVPMVRAIIAVAHSLQLDVLGEGVEQEQQRAILMAEGCDQA
                     QGYLFGHPMPAAEFELLLPTRSFPKAS"
BASE COUNT          910 a         1228 c         1107 g          937 t
ORIGIN      
        1 agcgctaaac acttgaaaag tcgatagatt aatcaccttc atgctcaggc agcctcctat
       61 tgttagacag agagttcgct ccctcctttg accctcgacg ggcttttcgg ctactctcga
      121 tcgacccggt taaacgcatc atgaatcgag cagatcgacg ccgtcaagac gcgtcccgag
      181 agccgcgaca tcaattcacg gtccaggcct ggctcgacca aggccgggtt catcaacagg
      241 ccggtcgact catcgaggcg gaagacgctt accggcacgc cctcgagctt gccccgcatg
      301 atgccgagtc cagccatcta ctgggtctgg tcacctaccg cctaaaccgt cttgctgaat
      361 ccctccgtta cctccatgct gcagtcgaac gacaaccttc ttcgcctgtc tactggttca
      421 atctcggcgt ggtatcgcaa aaggccggcc actcgccgga tgcagtatcc gcctatgaaa
      481 aagctatcgc gctcaatcct cgttatcctg aggcctatgg gaatttggga aatgttcttc
      541 gcgagactgg agatctgcca ggggcagtga acgcgtatga gcatgccttg aaactcaatc
      601 cttcacatgc cgacacgcat aataatctgg gggttgcaaa aaaagagcaa ggccagacca
      661 gggatgccgt tgcctgctac cgccgcgccc tcgaattgag gccgacccat gctgaagctt
      721 tgaacaacct cggcttggcg ctgatggaaa tcggcacgct tcacgacgcg atcggatgct
      781 ttcagcaagc gctctccatc ctccccggct atcaattagc ccgatacaat ctcggcatgg
      841 catggtcatg ggctggtgat gagatccaag cgtgccgctg tttgcacgag gtcgcgcgag
      901 ccaggcatga tcaccgttca gtaacggccg acacctccat ctatcggtcg cggatcaagc
      961 atgataccga gcaggtgcag ttcttgttcg agcaaggcaa actgggtgag gaccatcgtc
     1021 cgtatgcaca ggccttgcgc cagctgcacg aacggttgga aagcgattct agccccggaa
     1081 accgtctaat ggtcgcgccg agtgagctca tagccatcgc gccatcattt aatcgcattc
     1141 tgaattgtga accgcctccc agaatcgatc atggcgcatt gaatccattg ctcgatgtcg
     1201 ctgcggtgga agcgcagtac cacgccacgc gtcccgaggt gacctatatc gacaatctct
     1261 tgagccaggc cgccctcgac gcgttgcgca ggttttgctt ggaagcgacg atatggaaga
     1321 aagattatga gaatggctac agcggcgcgt tccttggcga cggatttgcc tcacccttgc
     1381 tttttcagat tgcggacgag ctgcgccgga agtttcctcg gattttttcg gtccaccgca
     1441 tgactcaagc ctgggccttc aaacatgaca gcgccaggcg tggactgaac attcatgccg
     1501 atgccgcggc ggtgaacgtc aatttctgga ttaccccgga cgaggcaaac ctgaatccag
     1561 cgagcggcgg gctcgttgtg tatgacaagg aagcgcctct ggaatggaat ttcaaggagt
     1621 acaacagcga ccgcaacaaa cccaagattt tgacatggct caaggaggcg ggggcgcagg
     1681 cgatcaagat tccctaccgg tcaaaccggg cagttgtctt caattcggat ttgttccatg
     1741 aaaccgatga cctgtctttc aaggacgact atctcagccg tcgcatcaac atcacgctcc
     1801 tctatgggta tcgctccaaa tcctgactag gatgccttgg gaaaggaacg cgtcgggagg
     1861 agcagttcga actccgctgc cggcattgga tgtccgaaca agtatccttg cgcttggtcg
     1921 cacccttcgg ccatcagtat cgcccgttgc tgctcctgtt cgaccccctc cccgagcaca
     1981 tccagttgga ggctatgagc caccgcgata atcgcgcgca ccataggaac cggttcggtt
     2041 ttgatgagta tttcccggat caatgagtga tcgattttca ctcggttaaa cggcaaccgt
     2101 tgaagatagc tcagcgaaga atgtcccgtc ccgaagtcgt caatcgacaa ttgcacgccc
     2161 aggtttttca attgatgcag gatcgtcagc gatgcctcta gattgcgcat cgcgatcggc
     2221 tccgtcagct ccagttcaag ccgattggca gtaagacccg tttcctccaa ggccgcctga
     2281 acggctctta agagcgtgct gccgtggaac aatgagtttg atacgttgac ggagacgctc
     2341 ggaacggtga ggccgcgctg ctgccaggcc cgcgcctggc ggcaagccgt tcgcagcacc
     2401 cattcgtcga ttgcgcggat gattcccgta tcggatgcag cctgaagaaa ttcattgggt
     2461 cccagcagcc cgcgctgcgg atgcagccat ctgatcaaca cttccgcacc gacaatcctg
     2521 ccgctcctca agtcgacctg cggttgataa taaaggacaa actcttcccg ttccgtcgct
     2581 tggcgcacct cagcttcgag catcaagcgt tcattagctg ctgcgttcat cgcattggaa
     2641 tagtattgaa agttgttacg accttgctcc ttggcgtgat acatcgccgt gtctgagctc
     2701 ttgagcagga gatccacgga gtcgccgtcg gtcggaaaga tggcgattcc gatgctcacg
     2761 gtcacgaaaa cttcatgtcc gctgatggag aacgggcggg ccagggcttc aaggatccgt
     2821 ctggccactt tgccagcgtc ttggacatct cgtagattcg tcaacagtac cgtgaactcg
     2881 tcaccgccca ggcgagccag ctcatgattg cgatcggtat ctgtcgtccg accgatggta
     2941 tcactgtgac gaaccgaatc catgaggcgg tccgcgacgt gtttgagcag taaatcaccg
     3001 atgtgatgtc ctaacgtgtc attggtaatc ttgaaacggt ccaagtccag aaagaggagc
     3061 gccaaattat attggtagcg gcgggcatga gcgatcgcct gcgtcacgcg atccttgaac
     3121 agcactcgat tggggaggcc tgtcaggcta tcgaaatatg cgagcctgtg gatttcccgc
     3181 tcggaccgtt tgcgctcact gatgtcctgc gccgttccca cgatggtcaa ggctttcaat
     3241 tgatcgtcga agaccgcctc ggcctgcaag ttcaccgtga actcacttcc attcggcaac
     3301 acgatgcggt gatcaatgtc gcatggcgtc cgctcggtaa gaatctgtgt aagcgcgtga
     3361 tggaaccgtt cacggtcctc gatgtcgacg gcttgtagat acgcgtccag cgtgccacca
     3421 aaatcttgcg gccggattcc catcagccgg cacagttcgt aagaagctga gaatccccca
     3481 ctttccggtt gccattccca gttgccaatc ttcgcaatcc gctgcgcgag acccagccgg
     3541 gcttcgcttc gcagtaacgc cctcaaggtc tgactcccgc gcaagagata ccgtattcgc
     3601 tggctcaaaa tcgtcgcatt gagcggcttg gcgataaaat cggtcgcacc gtgttcatac
     3661 gccatggcaa tcgcatcggt atcgtcgaga ccggtcatca tcaaaatggg gattttgccg
     3721 cctccggcct ccccgcgcag ctttgaacaa gccgcaaatc cgtccatcac cggcatcatg
     3781 acatccaaca caatgacgtc aggatgccga agggtaaatt gctccaacac ctctctgccg
     3841 ttcgaagcct cacagacttc gagcccgatc tgctctatcg ccgcacggac gaactgcctg
     3901 acagagttgt cgtcgtctgc caccagaacc atggcaggct tctcactgtt atccatgtgg
     3961 atgttccccc cagtctcccg attgagtcag ggacgaagca tcaagtgctg cggtcatggg
     4021 gcctcctaca tattcgtaag tatttgtctg atcgcttcac accgctcttc tccacgcttg
     4081 tgctccatgc acccagagtt gccggatctg cgagcggatt gtgaattgag tcttttcttt
     4141 gtgggacagc cttagcctag caaaacggct tggaatcgct ag
//
LOCUS       DASTQY010000477         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1331827, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000477 DASTQY010000000
VERSION     DASTQY010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331827"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..2408
                     /locus_tag="VFR82_15310"
     CDS             231..2408
                     /locus_tag="VFR82_15310"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEU4503017.1"
                     /translation="MGKTPKEKNAKAKDLATFQDNPDGQYLTTNHGLRVNDDQNTLKA
                     GERGPSLLEDFHLREKITHFDHERIPERVVHARGAAAHGFFQVYEPMASLTKAKFLQD
                     PDHKTPVFVRFSTVVGSRGSTDLARDVRGFAVKFYTEEGNFDLVGNNIPVFFIQDGVK
                     FPDLVHAIKPEPHREIPQAASAHDTFWDFISLMPESLHMIMWVMSDRAIPRSYRMMEG
                     FGVHTFRFVNEKGKSRFVKFHWKPVLGVHSVVWDEAQKISGLDPDFHRRDLWEAIENG
                     DFPEWEFGVQIVEEKDEQKFDFDLLDATKLIPEELVPVRRIGKLTLNRNPENFFAETE
                     QVAFHPGHVVSGIDFSNDPLLQGRLFSYTDTQLIRLGGPNFHEIPINRPIAPVHNNQR
                     DGHMRQTINQGRVSYQPNSLGGGCPMQAQANVGGFVNYAEEIKGTKVRGRSEKFFDHF
                     SQAKLFYNSQSAPEKAHIVQALQFELGKVEVSAIRARMLGLLTQVDKTLAGLVAQGLG
                     MTIAKLEGPMNMSVPADGHIKDFQPVHANGSLERSPALSMASPAKSGIATRKVAVLAA
                     DGFDDADLSNIKKAVTAAGGQVRIIGPRLGSLKGAKGTELPVDFSFLTAGSVLFDAVY
                     VPGGESSVASLMKIGKALHFLTEAYTHCKTIAATGGGAALVTAALLGPDEMVKPQPER
                     TPLDADPGIILYGASTAPMAAQFITAIGKHRHWEREAKGQVPA"
     gene            2467..3060
                     /locus_tag="VFR82_15315"
     CDS             2467..3060
                     /locus_tag="VFR82_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503018.1"
                     /translation="MKKEQLHELLFQALETEVGGVQVYETALQCVVNDDLKGEWQKYL
                     DQTRTHEKLLRDLLGEMGLDPAEETPGRQVVRHQGEALVEAMNMALKAGKSQAAQLVA
                     AECVVTAETKDHQNWELMGEAADQLPTTEGKALKAACEKVEDQEDEHLYHSMGWSREL
                     WMDSLGLAAVLPPPEEEMNVKTAAAAARAKKSRKQMV"
BASE COUNT          801 a          891 c          896 g          684 t
ORIGIN      
        1 agtgtcgcac gctcttttct ccggtataac ggcttttcag tcgacgatga cttcgtcagc
       61 ttgcatcgcc caattcgcgt tcagcctaag cacgccgcgc agtagacctg gctacctggg
      121 aacagagata ctaggcatcc caatctcatg agcaagggct ctacccactt cgctcctccc
      181 cagtttcatg cgaccataaa aacatcgcga ctggacttat gggagatcga atgggcaaaa
      241 caccaaaaga gaagaatgcc aaggccaaag acctggctac atttcaggac aatcccgatg
      301 ggcagtatct caccaccaat catggactcc gcgttaacga cgatcaaaat acgctcaaag
      361 ccggtgagcg cggaccctcc ctgttagaag acttccatct tcgtgaaaag atcacccact
      421 ttgaccatga acgtatcccg gaacgtgtag tgcatgcacg aggagcggcg gcccacggat
      481 tcttccaagt gtacgaacct atggcatccc tcacgaaggc gaagtttctt caagatccgg
      541 atcacaaaac gcccgtattt gttcgcttct cgacggtggt gggatctcgc ggttcgactg
      601 acctcgcgag agacgttcga ggctttgctg tgaaatttta tactgaagaa ggcaattttg
      661 atctggtcgg caataacatt ccggtcttct tcatccagga tggcgtgaag tttcctgatc
      721 tcgttcatgc catcaagccg gagcctcacc gagaaatccc tcaggccgca tcggcgcacg
      781 ataccttttg ggactttatt tccctgatgc cagaatcgct gcacatgatt atgtgggtca
      841 tgtcggatcg ggcgatcccc cgaagttacc gcatgatgga gggcttcgga gttcacacct
      901 ttcggtttgt gaatgaaaaa gggaaatcac ggttcgtgaa gtttcactgg aagccggtat
      961 tgggcgtgca ttcggtcgtc tgggatgaag cacagaaaat ttctggcttg gaccccgact
     1021 ttcaccggcg cgatctgtgg gaagcgatcg agaacggtga ttttccagaa tgggaattcg
     1081 gggtgcagat cgtcgaagaa aaggatgaac agaagttcga cttcgatctc ctcgacgcaa
     1141 cgaagcttat tccggaggag ctcgtaccgg tcagacgaat tggcaaactg actttgaacc
     1201 gcaatcccga gaattttttt gctgagactg agcaggtcgc ttttcaccca gggcatgtcg
     1261 tctcaggcat tgacttttca aacgatccac tgctgcaagg ccgcctcttt tcctacacgg
     1321 acacgcaact catccgttta ggtggaccca attttcatga gattcccatc aaccgaccca
     1381 tcgcgcccgt gcacaacaat cagcgggacg gtcatatgcg tcaaacgatc aaccaaggtc
     1441 gcgtcagtta ccagccaaat tcattgggcg gcggatgccc gatgcaggcg caagccaatg
     1501 tgggcggatt tgtcaattac gccgaagaga tcaaagggac gaaggtccga ggccggagtg
     1561 agaagttctt tgaccatttc agccaggcca aactgtttta taacagccag tccgcgcccg
     1621 aaaaagccca tatcgtgcag gcgctccaat tcgaattggg caaagtggaa gtctctgcga
     1681 tccgcgcacg catgctcggg ctcttgactc aggtggataa gacactggcc ggcctggtcg
     1741 ctcaaggtct cgggatgacc attgccaaac tggaagggcc gatgaatatg agcgtgcccg
     1801 ccgatggcca tataaaggat ttccaaccgg tgcacgccaa tggctcgctg gaacgttcac
     1861 cggctctgag catggcatcc ccggccaaga gcggtattgc cacgcgcaaa gtggccgtgc
     1921 ttgcggctga cggctttgac gatgcggatc tctcaaacat caagaaggcc gtgacggccg
     1981 ccggaggcca agtaagaatc attggcccgc gacttggcag cctcaagggc gcaaagggca
     2041 ctgagcttcc tgttgatttc agtttcctga ccgcagggtc cgtattattt gacgccgtct
     2101 atgtgcccgg cggggagagc agcgtggcga gcctgatgaa gatcggcaaa gcgctccatt
     2161 tcctgaccga agcctacacg cactgcaaga ccattgctgc aaccggggga ggcgcggcac
     2221 tggtgaccgc cgcattgctc ggaccggatg agatggtgaa gccccaacct gagagaacgc
     2281 cgctggacgc ggatccgggc atcatcctct atggtgcctc cactgctcct atggccgcgc
     2341 aattcatcac tgcgatcggc aaacatcgac attgggaacg cgaggccaaa ggccaagtcc
     2401 ccgcatgatc tatccgctgt gagtgccgtc atcctatcaa ccattccgaa tcagagagga
     2461 tcttccatga agaaagaaca attgcatgaa ctcttattcc aagcgttaga aacagaagtc
     2521 ggcggggtcc aagtgtatga aactgccctc caatgtgttg tcaatgacga cctcaaaggg
     2581 gaatggcaga agtatctcga tcagacccgt acccatgaaa aactcctacg cgatctactg
     2641 ggcgagatgg gtctagatcc ggcggaagag acaccgggac gacaggtggt caggcatcaa
     2701 ggcgaagcgc ttgtggaagc gatgaacatg gcactgaaag cagggaaatc ccaggcggct
     2761 caactcgtcg ccgcggaatg cgtggtcacg gcagagacca aggaccatca gaattgggaa
     2821 ctcatgggtg aagcagccga ccaactgcca acaacggaag gcaaggccct gaaagctgcg
     2881 tgtgagaagg tcgaggacca agaagacgag catttgtacc atagcatggg ctggagtcgt
     2941 gaactgtgga tggactctct cggattggca gcagtccttc cgcctccaga agaagagatg
     3001 aacgtcaaaa cagcggcagc tgccgctcga gccaagaaat ctcggaagca gatggtgtaa
     3061 gacgacaaga cgctcgctct agtatctccg gcaagaggga ggctgtagcc gaggacctca
     3121 gccggagaac tcgctcctga cgaacgaaga gatgtctcct cagatacttt ccaccgcctt
     3181 cttgattctg tgcgggctcg tgctctatca gttggcgttg atcttcgcac cttttcttac
     3241 gcccatccta tgggccctca tcctggcgcg tc
//
LOCUS       DASTQY010000478         5924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1120556, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000478 DASTQY010000000
VERSION     DASTQY010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120556"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VFR82_15320"
     CDS             <1..657
                     /locus_tag="VFR82_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503019.1"
                     /translation="TLRAFDARNGKPVASIHVADGKAMVHLTDVAYDGVGLLYCSDQH
                     ANRIYRVELPSQKVTLLVADPALAGPAGLAVHPKSGELIAVSWNKGKIFAISPNGTVS
                     ELFSNGFFSSRFSNLRGVDFDRWGNMYVSDFTTGKVWRMTWDKRFQVIAEYLPSPGDL
                     SIDRTNNLILVPYEFAHAAEMNGLEAPRDGKSKSEKRTLADYGFIPPPPKPAEGAPKQ
                     "
     gene            654..1349
                     /locus_tag="VFR82_15325"
     CDS             654..1349
                     /locus_tag="VFR82_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503020.1"
                     /translation="MSACAYCHQRKGKRPCPALGVICSQCCGEHRLVRVSCPPDCAYL
                     GAGSDYQQKRLSEQFMPVRREFYREISEVGGEKAVALYNLIEVVTFSYFHARRDGQDA
                     EVVTAIQALRRTLSPLHVPSTPMPVFAEHLKKEYETFKKQNPQQIADVSTAPDVLDRA
                     ITFVSEFSGNDFQSRRFVTGLVGYVKMYHPEIAAHLTKQHQAGHIVLPGQSFLPPAAT
                     EPHTHGPGCAHHH"
     gene            1362..1682
                     /locus_tag="VFR82_15330"
     CDS             1362..1682
                     /locus_tag="VFR82_15330"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4503021.1"
                     /translation="MTVERAKKVRKSRRVDLRCQLGFSSEEIEGDAHVTNISTGGCRA
                     ESEVNVAEGLQFVVLLHLPGQTPLKIERASVRWVKGNSFGLSFILFLPSERAKLQNYL
                     EAIK"
     gene            complement(1698..2288)
                     /locus_tag="VFR82_15335"
     CDS             complement(1698..2288)
                     /locus_tag="VFR82_15335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU4503022.1"
                     /translation="MIITAMIMAMNVFAGCSGKSDVKAPPPLPDPRPRAIIKTRFGEM
                     ELKLYPDLAPKHVENFINLAKSGFYNGTIFHRVIPGFMIQGGDPNTKYSLRKDTFGQG
                     GPKDEKGNPILLKAEFSDTPHKRGIVSMARAGEPDTAGSQFFIIVEDSFFLDRKYTVF
                     GEVTRGLGVADKIANLPRNERDLPNDRIEMTVTILE"
     gene            complement(2340..2921)
                     /locus_tag="VFR82_15340"
     CDS             complement(2340..2921)
                     /locus_tag="VFR82_15340"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU4503023.1"
                     /translation="MQRLVMTLAMAMSVMTAAWALVGPASVAGETGGGKASKAVIKTK
                     FGDMEIAFFPDKAPNHVQNFIKLAKSGYYNGTIFHRVIPGFMIQGGDPNTKDPKKPET
                     YGMGGPSDRLKAEFNDTPHRRGIVSMARSNDPNSAGSQFFIVVKDSNFLDGQYTAFGE
                     VVKGMEVADKIVNLPRNARDLPNERAEMTVVVE"
     gene            complement(2976..3683)
                     /gene="rnc"
                     /locus_tag="VFR82_15345"
     CDS             complement(2976..3683)
                     /gene="rnc"
                     /locus_tag="VFR82_15345"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU4503024.1"
                     /translation="MTPAPPLDSLQAALAYRFINPVLLQEALTHKSFINEQKTTTGSH
                     NERLEFVGDAVLSLVVSEQLAALLPQSSEGALSKHKARLVSESMLADVARRLKVGDCL
                     RLGRGEELSRGREKDSLLADALEALIAAVYLDGGLESSRRVVTGLLEHELRRVVTQQH
                     RPGEDDYKTQVQEWCQRRFDSLPSYTVVRESGPDHDKLFEVEVAVNGKVVGRGTGRSK
                     KEAEQFAAKQALHEAGP"
     gene            complement(3717..4970)
                     /gene="fabF"
                     /locus_tag="VFR82_15350"
     CDS             complement(3717..4970)
                     /gene="fabF"
                     /locus_tag="VFR82_15350"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU4503025.1"
                     /translation="MKSDGAKRRVVVTGLGLITPLGTGVDKTWKAICSGQSGIGRITK
                     FDPAGYDAQIAGEVKDFDPAQFIEKKEIKKMDAFIHYAVGAAQLAADDAGLKVLPEEA
                     TKVGVYIGSGIGGLGSIEHYHTVLKDKGPGRVSPFFIPMTIINLASGQVAIRLGAKGP
                     NSCAVTACATGNHCIGDAFRLIQRGQADVMVAGGAEAAITTLGVAGFAASKALSFRND
                     EPTKASRPFDKDRDGFVLGEGAGVIVLEELEHARRRGVRIYAEVVGYGMNSDAYHITA
                     PSEEGEGAVRCMELALADADMGKDEVSYINAHGTSTMADVIETKAIKQVFGERALRIP
                     VSSTKSMTGHLLGAAGGIEAVFSILAIHHGILPPTINLDHPDPECDLDYVPHKARPAT
                     VNVALSNSFGFGGVNACLLFKKPEA"
     gene            complement(4980..5213)
                     /gene="acpP"
                     /locus_tag="VFR82_15355"
     CDS             complement(4980..5213)
                     /gene="acpP"
                     /locus_tag="VFR82_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU4503026.1"
                     /translation="MATVDERVKKIIAEQLGVEEDEVTSEASFVEDLGADSLDTVELV
                     MALEEEFGIEIPDEDAEKILTVGKALDYIKEKS"
     gene            complement(5322..>5924)
                     /gene="fabG"
                     /locus_tag="VFR82_15360"
     CDS             complement(5322..>5924)
                     /gene="fabG"
                     /locus_tag="VFR82_15360"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU4503027.1"
                     /translation="AIEKIGRKALNVKVNVADSNDTKTMADQVLKQWGTIDILVNNAG
                     ITRDGLLLRMKEEDWNLVLQVNLNGTFHCTKAVLQPMTKQRYGRIVNIASIVGAMGNV
                     GQANYAASKAAVIGFTKTAAREYASRNVTVNAVAPGFIDTAMTQGLSPEVKEALQKQI
                     PLARLGTPADIANAVRFLVSEDAAYITGHVLHVNGGMLMS"
BASE COUNT         1226 a         1767 c         1567 g         1364 t
ORIGIN      
        1 acgttgcgtg cattcgacgc acgcaatggg aaaccggttg cctcaatcca tgtggccgat
       61 gggaaggcaa tggttcattt gactgacgtt gcttacgacg gcgtaggact actgtactgc
      121 tcggatcagc atgcaaatcg aatctatcgt gtcgagttgc cctcgcagaa ggtcaccctg
      181 ttggtggccg atcctgcgct agccgggccc gcggggttgg cggtgcatcc aaaatccggc
      241 gaactcattg ccgtcagttg gaacaaaggc aaaatatttg cgatcagccc gaacggcaca
      301 gtctccgagc tcttttccaa cggctttttc tccagccggt tctcgaacct gcgtggagta
      361 gacttcgatc gctggggcaa catgtacgtg tccgacttca cgaccggcaa ggtctggcgg
      421 atgacctggg acaaacggtt tcaggtcatt gccgagtatc tgccttcgcc tggcgacctg
      481 agcattgatc gcacgaacaa tctgatcctg gtcccctatg agttcgccca tgcggccgag
      541 atgaatggtc tggaagcccc ccgtgacggc aagtccaaat ctgaaaagcg gacgctcgcg
      601 gattacggtt tcattcctcc gccaccaaag ccggcggaag gagcgccaaa acaatgagcg
      661 cgtgcgccta ttgtcaccaa cgaaaaggca agcggccttg tcccgcgtta ggggtcatct
      721 gcagtcagtg ctgcggggag caccgtctcg tgcgggtctc gtgcccgccg gactgtgcgt
      781 accttggcgc gggcagcgac tatcaacaaa agcggttgag cgaacagttc atgcccgtcc
      841 gacgggagtt ttacagggag atcagcgaag taggaggcga aaaggcggta gccctctaca
      901 atctgatcga agtcgtcacg ttcagttact ttcacgcccg tagagacggt caggacgccg
      961 aagtcgtcac cgcgatccaa gcgttgcgac gcaccttgag cccgctgcat gtgccctcga
     1021 cgccgatgcc tgtcttcgcg gagcatctca agaaagagta cgagaccttc aagaaacaga
     1081 accctcagca aatcgccgac gtctcgaccg ctcccgatgt ccttgatcgt gccattacat
     1141 tcgtgtccga gttctccgga aatgactttc aatcgcggcg atttgtgact ggactagtcg
     1201 ggtatgtgaa aatgtaccac ccggagatcg cggcacactt aaccaagcag catcaggccg
     1261 gccatatcgt cctccccggt cagtcgttcc tgccgccggc ggcgactgaa ccgcatactc
     1321 atggaccggg atgcgcgcac catcactaga ggttgactgc aatgaccgtc gaaagagcga
     1381 agaaagttcg aaaatcccgt cgtgtggacc tgcgttgcca acttgggttc tcctccgaag
     1441 aaatcgaagg agacgcccat gtgacgaaca tctccactgg agggtgccgg gcggaatctg
     1501 aagtcaacgt cgcggaggga ttacagtttg tcgtcttgct tcaccttccc ggacaaactc
     1561 ctctcaagat tgagcgtgcc tccgtacgct gggtcaaagg caacagcttc ggattgagct
     1621 ttatcctctt cctgccatct gaacgcgcga aactccaaaa ctatctggaa gcgatcaagt
     1681 agctcccgat agaccattca ctctagaatc gtcactgtca tttcaatccg atcgttcggg
     1741 agatcccgct catttctcgg caggttggcg atcttgtcag ccacacccaa tcccctcgtc
     1801 acttcgccaa aaacggtgta cttccggtcg agaaagaacg aatcctcaac aatgatgaaa
     1861 aactgagacc cggcagtatc aggctcgccg gctcgcgcca tcgacacgat gcctcgcttg
     1921 tgcggcgtat cgctgaattc agccttcaac agaattggat tacccttttc gtccttgggt
     1981 cccccctggc cgaacgtgtc cttcctgagc gagtacttgg tattggggtc gcctccctga
     2041 atcatgaagc ctggaatcac gcgatggaaa attgtcccgt tatagaaacc ggacttcgcc
     2101 aagttgatga aattctcaac gtgcttaggg gcgagatctg gatataactt aagttccatt
     2161 tctccgaacc gagtcttgat gatggctctc ggacgagggt ccggaagagg cggcggcgcc
     2221 ttgacgtcgc ttttcccgga gcagccggca aagacattca tggccatgat catagcggtg
     2281 atgatcacga gaccgcttcc ttttcccact agccgtctcc tccctgcccc cggaccatgc
     2341 tactcaacga cgacggtcat ctcggcgcgt tcgttcggga ggtcacgcgc attcctcggc
     2401 aaattgacga tcttatcagc cacttccatt cctttcacga cttcgccgaa tgccgtgtat
     2461 tgcccgtcca gaaagttgga atctttgacg acgatgaaga attgcgagcc cgcactattg
     2521 gggtcgttac tgcgcgccat cgagacaatg ccgcggcgat gcggtgtgtc attgaactct
     2581 gctttgagcc tgtcgctcgg tccacccata ccgtaggtct cgggcttctt cggatctttc
     2641 gtgttaggat cacccccctg gatcatgaaa cctggaatca cgcgatgaaa aatcgttcca
     2701 ttgtaatagc ctgacttggc cagtttgatg aaattctgaa catggttcgg ggccttgtcg
     2761 gggaaaaatg cgatctccat gtctccgaat ttagtcttga tcaccgcttt tgacgctttc
     2821 cctccgcccg tctccccggc aactgaagcg ggtcccacga gagcccaggc agctgtcatg
     2881 acgctcattg ccattgccag tgtcatcacc agtctttgca tgtctgtacc tccatgattc
     2941 gtatgtggcc tttatcatac cagccccgtc gaccgtcaag gtccggcttc atgcaatgct
     3001 tgttttgctg caaactgctc tgcttccttc ttgctgcgtc cggttcctct gccgactact
     3061 ttcccgttga cggcaacttc aacttcgaag agtttgtcgt gatcaggtcc cgactcacga
     3121 acgactgtat atgagggaag actatcaaag cgtcgctgac accattcctg gacctgcgtc
     3181 ttgtaatcgt cctcacccgg acgatgctgc tgggtgacca ctcgcctcaa ttcgtgctcc
     3241 aacagtccgg tcacgacgcg acgggatgat tcgagtccgc catcgagata caccgcggcg
     3301 atgagggctt ccagcgcatc agcaagcagc gaatcttttt cgcgtcctcg tgacagctcc
     3361 tcgccgcggc caagccgcag acagtccccc actttgaggc gtctggcgac atcagccagc
     3421 atcgattcac tgaccagtcg agccttatgc ttcgaaaggg cgccctcgga tgattgcggc
     3481 agcaacgccg cgagttgctc actgacgaca agcgacaaca cggcgtcccc cacaaattcc
     3541 agccgctcgt tatgcgagcc cgtcgtggtc ttctgctcgt tgataaatga cttgtgcgtc
     3601 aaggcctctt gaagcaacac gggattgatg aaccgatagg caagggcggc ttgaagcgaa
     3661 tccagcgggg gagccggtgt catacaaata ggcagacgcc ccgaggcgtg aatccattaa
     3721 gcttcgggtt ttttgaagag caggcaggca ttgaccccgc cgaatccgaa cgaattcgag
     3781 agtgccacat tgaccgtcgc aggtctggcc ttgtggggga cataatccag atcgcattcc
     3841 ggatccgggt ggtcgaggtt aatggtcggc gggagaatac cgtggtgaat ggccagaatg
     3901 ctgaataccg cttcgatgcc gccggcggcg cccagcaggt gcccggtcat cgacttcgtg
     3961 gaacttaccg ggatgcgcaa ggcccgttca ccaaagactt gcttgatcgc cttggtctcg
     4021 atgacatccg ccatcgtcga tgtgccatgg gcattaatgt agctgacctc atctttcccc
     4081 atatcggcat ctgccagcgc caattccata caccgcacgg cgccctcacc ttcttcggaa
     4141 ggagcggtga tgtgataggc atcgctattc atgccgtaac ccaccacctc ggcgtagatg
     4201 cggacgcccc gccgccgagc atgttccagc tcttccagca cgatcacgcc ggcgccttca
     4261 ccgaggacaa atccgtcacg gtctttatca aacggacgac tggctttcgt cggctcgtca
     4321 tttcggaacg agagcgcttt tgacgcggca aaccccgcga cgccaagtgt agtaatcgcg
     4381 gcctctgcac cgccggccac catcacgtcc gcttgaccgc gctggataag ccggaatgca
     4441 tcgccgatgc aatgattccc tgtcgcgcac gccgtgactg cacaggaatt gggtcccttg
     4501 gcgcccaacc gaatcgccac ctgtccggag gcgagattga tgatggtcat cgggataaaa
     4561 aacggcgaga cccggccagg ccctttgtct ttgaggaccg tatggtagtg ctcgatggat
     4621 cccaacccgc cgatccctga gccgatatag actccgactt tcgtcgcctc ttccggtaag
     4681 accttgagtc cggcatcatc cgcggccaat tgggctgcgc ccaccgcata gtggatgaag
     4741 gcatccattt tcttgatttc ttttttctcg atgaattggg cgggatcgaa atccttgacc
     4801 tcaccggcga tttgggcgtc atagccggcg ggatcgaact tggtgatccg gccgattccg
     4861 gattggccgg agcagatcgc tttccaggtt ttgtccacgc cggtgccgag aggggttatc
     4921 agcccgagac cggtgacgac aacgcgtctc ttcgcaccgt cgctcttcac gttcaacctt
     4981 tacgattttt ccttgatata gtccaaggcc ttgccgacag tcagaatctt ttccgcgtcc
     5041 tcgtcgggaa tttcaattcc aaattcctct tccagcgcca tgacgagttc gacggtatcc
     5101 agtgaatcgg cgcccaggtc ttcgacgaag ctggcttccg acgtcacttc atcttcctcg
     5161 actccaagtt gttcggcgat gattttcttc accctttcgt ctactgtagc cataaacgtg
     5221 caatcctcct tccagatctg tcgttcccca actgtctcat ccgccgcgga cattgtccgc
     5281 agtatgtggt ttgtcatcca agctcaggcg gccaaggtct atcaagacat gagcatgcca
     5341 ccgttcacat gcagcacatg ccccgtaata taggccgcat cctcagagac tagaaagcgc
     5401 acggcgttcg caatgtccgc cggcgtgccg aggcgggcaa gaggaatttg cttttgcagc
     5461 gcctccttga cctcaggaga caagccctgc gtcatcgccg tgtcgatgaa cccgggagcc
     5521 accgcattga cggtgacgtt ccggctggcg tattcacgcg ccgccgtctt cgtaaagccg
     5581 atgacagctg cctttgacgc cgcgtaattt gcctgtccca cgttccccat tgcgccgacg
     5641 atcgaggcaa tattcactat tcgaccatag cgctgtttgg tcatgggctg caagaccgct
     5701 ttggtgcagt gaaatgtgcc gttcaaattg acctgaagca ccagattcca atcttcttcc
     5761 ttcatccgta gtagcagccc gtcgcgggtg atgccggcat tattgaccag gatatcgatc
     5821 gtcccccact gtttgagcac ctggtcggcc atggtcttgg tgtcattgga gtctgccacg
     5881 ttcactttga cattgagggc tttcctgccg atcttctcga ttgc
//
LOCUS       DASTQY010000479         4296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1397527, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000479 DASTQY010000000
VERSION     DASTQY010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397527"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFR82_15365"
     CDS             <1..227
                     /locus_tag="VFR82_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEU4503028.1"
                     /translation="RELGISLMEMRNRRRQAIDEALTRLNEGTYGICAECGVEISERR
                     LEAVPFAKLCVECQAREELLEKIEKEEERD"
     gene            328..1029
                     /gene="queC"
                     /locus_tag="VFR82_15370"
     CDS             328..1029
                     /gene="queC"
                     /locus_tag="VFR82_15370"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEU4503029.1"
                     /translation="MTGTQTPTRAVVLASGGLDSTVTAALAREAGHELMWLTVDYGQR
                     HAVEIERARRLAAAMSVARHTVVSVDLRAIGGSALTSDVAVPKDRPSADRSHGIPTTY
                     VPGRNLIFLSLAAAHAEVVGAPVIYFGANVLDYSGYPDCRPEFIAAFEEAVRAGTKAG
                     AENLRLRVCAPLLTMSKAEIIQTGVRLDVPFHLTHSCYDPVGDLACGRCDSCLIRRDG
                     FSNAGVVDPTAYAVT"
     gene            1077..1244
                     /locus_tag="VFR82_15375"
     CDS             1077..1244
                     /locus_tag="VFR82_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503030.1"
                     /translation="MMAVSPEEFFMYLTIGLIVIAPIAARLYRRMTLRRTQRMLREQF
                     TEPARGQGERQ"
     gene            1241..1636
                     /locus_tag="VFR82_15380"
     CDS             1241..1636
                     /locus_tag="VFR82_15380"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU4503031.1"
                     /translation="MRTGSAVLHVSLLALTGMLAIIACDSSQPKSAVGGATVPAELQR
                     GETKFHAHCSACHGPQGTGTQQGPPLVHKIYEPNHHGDAAFQRAATFGVKAHHWEFGN
                     MPKIDGVTSADVEQITGYIRWLQRQAGIF"
     gene            1791..2624
                     /locus_tag="VFR82_15385"
     CDS             1791..2624
                     /locus_tag="VFR82_15385"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU4503032.1"
                     /translation="MRRAAGGPVIVLSIMMGFGSIGCAAIDAGHEGVLVEQPFFFGHG
                     GVDPVPSKTGRVWLAPTTHVVDVDVRPVQYSEHFDIISAENAPVSFDAFMIANVVEGR
                     SPELIARYGPQWYHNNVKEAFRTFVREEVQKYPLFQLTTDPTTRTKLQEAIAREVQTK
                     LIEKQNIPVRLNRVVVGSILPPKGVVEQTTQTIVQEQRKITMVEFQKAEEAREKAEKQ
                     RGIADRAYRESLGLTAPEFVDLRRIEVQKEIVQHSPSALTVIMGLERFGINLPPIGAD
                     K"
     gene            complement(2692..3954)
                     /locus_tag="VFR82_15390"
     CDS             complement(2692..3954)
                     /locus_tag="VFR82_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503033.1"
                     /translation="MVHAHYMKTVMGGLSLVLLLSACSSGSKSKTTTAPPSGDISRGA
                     TATISPPSDLPRSDVVSDRGTTRAKTYDSTSRAGLSDRSPEIAAACPSRTDAQGTVVF
                     EDPTCPDRFRRQAGATPASDGAGLAAACSSRVDANGKVIYDDPACPDRFRSQVGTTSE
                     KERAGLAAACSSKTDANGKVIYDDPACPDRFRQQNASMTAIREARVRLSRAAGIKTWD
                     SQPGDKLARPDPVGVAGDCPSKVDANGKVIYADSGCAHRYYYQTKENAYYDRYADKAA
                     KHARAAEIAAIEGNVPDMIQNAELSLDHAKEARRSGNNPDLAAGIAALRQSIALGQSN
                     EVPGSKSSPTSTVAAAPRTQTIKGELLRNEKSTKVGGGEEYIVRDSQHREMPIALSPE
                     MSQQVQVGDMVEAQVDSNGQVTSISKAQ"
BASE COUNT          905 a         1202 c         1265 g          924 t
ORIGIN      
        1 accgcgagct cgggatttcg cttatggaaa tgcgcaatcg acggcggcag gccattgacg
       61 aagcgctcac ccgtctgaac gaaggcacct acggtatctg tgccgagtgt ggggtggaaa
      121 tcagtgagcg acggttggag gccgtgcctt ttgcaaagct ctgcgtcgag tgtcaggcga
      181 gagaagagct tctcgagaag attgagaagg aagaagagcg cgactagttt cgcattcgcc
      241 ccgccatggt ttcgcattgc atgccgccgt ccgtccgttc cctcagtgtc agccctcggc
      301 tcggtacggt aaccgagatc tcacattatg accggcaccc aaacaccgac acgtgcagtg
      361 gtgcttgcga gcggcgggct tgactctacg gtcacggccg cactagcccg cgaggccggc
      421 catgagttga tgtggctgac cgttgattac ggacaacgtc atgcggttga aattgagcgc
      481 gctcgccggc ttgccgcagc catgagcgtc gccaggcata ccgtagtctc agtggatctc
      541 cgcgcgatcg gagggtcggc actgacgagc gatgtggcgg taccaaaaga ccggccgagt
      601 gccgaccgca gccatggcat tccgaccacc tatgtgccgg gccggaacct catcttcctt
      661 tctcttgccg cagcacatgc cgaagtggtg ggtgcacccg tgatctattt cggagccaac
      721 gtactggact attcagggta ccccgactgt cgacccgagt ttatcgcggc ttttgaagag
      781 gccgtgcgtg ccggtacaaa ggcgggcgcc gagaacctcc gtcttcgtgt ctgcgcacca
      841 ctgcttacca tgagcaaggc cgagatcatt caaaccggtg tccgcttgga cgtgcccttc
      901 cacttgactc acagttgtta cgatccggtt ggcgacctcg cgtgcgggcg gtgcgatagt
      961 tgtctgatcc gccgcgacgg cttctccaac gcaggggttg tggatccgac ggcctatgct
     1021 gtaacttgaa catgccttgc gctcgatcca ccggcgcgtt ggaacgctgt tgaaagatga
     1081 tggcagtctc ccctgaagaa tttttcatgt atctgacgat cgggttgatc gtgatcgcac
     1141 cgatcgctgc gcgactctac cgccgcatga cgctgcgtcg gacacagcgg atgctgcgtg
     1201 agcaattcac cgagccggca cgcgggcaag gagaacggca gtgagaacgg gatctgccgt
     1261 gctacacgtg tccctgctgg ccctcacggg gatgctcgcc atcatcgcat gcgactcatc
     1321 gcagccgaaa tccgccgtcg gaggagccac ggttccagcg gaattgcaac gtggagaaac
     1381 caaatttcac gcgcattgtt cggcctgcca tggtccacaa ggcactggga cacagcaagg
     1441 tccgccgctc gttcataaga tttatgagcc caaccaccac ggcgacgcgg cgtttcagcg
     1501 cgccgccaca ttcggagtca aagcccacca ttgggagttc ggcaatatgc cgaagatcga
     1561 cggagtcact tcggcagacg tcgagcagat caccggctat atccgctggc tgcagcgtca
     1621 agccggcatc ttctaagttc tcgtattcct tccactcgtt cgccttcgag ccggtttgac
     1681 tttgttcgca agcgcttcgt aaggtaaacc tacgagttcg aaaacgatgc ggcggggcat
     1741 gttgccaggt cgtccttcac tcacactaca atcaaagcac aggagggcac atgagaagag
     1801 cagccggagg tcctgtgatt gtgctcagca tcatgatggg atttggaagc atcggatgcg
     1861 cggcgatcga tgcaggacat gaaggtgtat tggtggaaca gccgtttttc ttcggtcatg
     1921 gcggcgtcga tccggttccg tcgaaaacgg gacgtgtgtg gctggcgccg acaacgcacg
     1981 tggtggatgt cgatgtccgg ccggtccaat attctgaaca tttcgacatc atctccgccg
     2041 agaatgcccc cgtctctttc gatgcgttca tgatcgcaaa tgtcgtggaa gggcgctcac
     2101 ctgagttgat cgcacggtat ggtccgcaat ggtaccacaa taacgtgaag gaggccttcc
     2161 gtacgttcgt gcgtgaagag gtgcaaaaat atccgctctt ccagttgacc accgatccga
     2221 cgactcgcac caaacttcag gaggccatcg cgagagaagt ccaaaccaaa ttgatcgaaa
     2281 aacagaacat ccctgtgagg ctgaaccgcg tcgtcgtcgg tagtatcctt cctccgaagg
     2341 gtgtggtgga gcaaaccacc cagaccatcg ttcaggaaca acgaaagatt acgatggtgg
     2401 aatttcagaa ggcggaggaa gcccgagaaa aagcggaaaa gcagcgtggc attgccgacc
     2461 gggcctaccg cgaatcgctc ggtctcacgg ctccggagtt tgtggatctt cggaggattg
     2521 aagtgcagaa ggagattgtc cagcactcac cgtcggcgct gacggtcatc atgggattgg
     2581 aacggttcgg aatcaatctg ccgccgattg gtgcagacaa ataagcataa ggaaggcaca
     2641 gagacagaga tggagccgtg aggtcacggc tccatcttgt gcgactaagg tttattgggc
     2701 cttcgaaatt gaggtcactt ggccgttgga atcgacctgc gcttcgacca tgtcacccac
     2761 ctgaacctgt tggctcatct caggcgacaa agcaatcggc atttccctgt gctgactgtc
     2821 gcggacaatg tattcttctc caccaccaac cttcgtagac ttttcgttcc gcagcagttc
     2881 gcccttgatg gtttgggtgc gcggcgcggc ggcaacagta ctcgttgggg atgacttcga
     2941 accaggtact tcattgcttt gtcccagtgc gatcgactgc ctcagggccg caatgcctgc
     3001 tgccagatca ggattattgc cggagcgcct tgcctccttc gcatggtcca atgacagttc
     3061 ggcgttttgg atcatgtccg gcacgttacc ctcgatggca gcgatctctg ccgcccgggc
     3121 gtgtttagct gctttatccg catagcgatc gtaataggca ttttcctttg tctgatagta
     3181 gtaccggtgc gcacagcctg agtcagcata gatcactttg ccgttggcat cgacttttga
     3241 aggacaatct cccgcgacgc cgacaggatc aggcctcgcc agcttatcgc caggctggga
     3301 atcccacgtc ttgatgccgg ctgcccggga gagtcggaca cgggcctcac ggatggcagt
     3361 catgctggcg ttctgttggc gaaagcgatc cggacaagcc ggatcatcgt aaatgacctt
     3421 gccattggca tcggttttgg aagaacaggc tgccgccagt cccgcacgtt ctttttcgga
     3481 ggtggtaccc acctggctgc gaaagcgatc cggacaagcc ggatcatcgt agatgacttt
     3541 gccgttggca tcgactcgtg aagaacaggc cgctgccaac cctgcaccat cgcttgcagg
     3601 ggtggctccc gcctggcggc gaaagcgatc cgggcaggtt ggatcctcga acaccacagt
     3661 gccttgggca tccgtccgtg atgggcatgc tgccgcgatt tccggcgagc gatcagaaag
     3721 ccctgctcgg gacgtcgaat cataggtttt ggctcgggtg gtgccacgat cgctgacgac
     3781 gtcgcttcgc ggcagatcgc tgggcggcga aatagtagcg gtggcccccc gcgatatgtc
     3841 tccagacgga ggagcagtcg tggtctttga cttggatccg gaggaacagg ccgacaagag
     3901 aagaacaagt gaaagaccgc ccatcacggt cttcatataa tgcgcgtgta ccataaagcc
     3961 tcctttatat gacgcagttt agccttagtc gggcatgacg ggctgccccg gggtccctgc
     4021 cgacaggcac cagatttcaa cctttctgtt caagctagtg ctcaatccga gataaggcca
     4081 ctaggcatat ccctgtattt cgatgaaagg aggatggttg tcatgcctgg gttcgacccc
     4141 tctatgtctc taataaaacg gctcgttcag gaaccagggg atatttcagt ggtcatcgag
     4201 cgactctccg gtaggctagt gacgaggata tgatcgatga agcgacgacc atcgcaggat
     4261 gtctgggaag cgtttcacat cgtgacagat gcagca
//
LOCUS       DASTQY010000480         5674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1323438, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000480 DASTQY010000000
VERSION     DASTQY010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323438"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(398..1018)
                     /locus_tag="VFR82_15395"
     CDS             complement(398..1018)
                     /locus_tag="VFR82_15395"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU4503034.1"
                     /translation="MAKRTPKKSKSSQPLDAVQLLKADHKQVRKLFEEFHGASDDDKG
                     SIARRLFTELEIHSKLEEELLYPAVQAKLESLDYETEGNGLDVSDDGERDQEAEGAMD
                     DIDGAELEVLEDETDEEAESEIITAAYDSHGTVRELIQQLQSLDPKSTDYREMFTELE
                     DAVIEHVAEEEDAILPLAAAQLDVLALGMTMQQRKDDLTSQSSLAA"
     gene            complement(1059..1922)
                     /locus_tag="VFR82_15400"
     CDS             complement(1059..1922)
                     /locus_tag="VFR82_15400"
                     /inference="COORDINATES: protein motif:HMM:NF005214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4503035.1"
                     /translation="MPDTKTQQRPPQHQSRQPGLETAMTPRPESESPEQRGCGKLRDK
                     VALITGGDSGIGRAVAIAYAKEGANVTIVYLNEHEDAEETRRMVEGEGRRCLVIAGDV
                     GDENFCVQTVQQTVREFGRLDILVNNAAEQHPQDSLEDITAEQLERTFRTNIFSYFYM
                     GKAALPHLKEGSAIINTTSVTAYKGSAQLLDYASTKGAIVAFTRSLSQALAEKKIRVN
                     AVAPGPIWTPLIPSTFPADKVASFGSDVPLKRAGQPEEVAPSYVFLASQDSSYTTGQV
                     LHPNGGTVVNG"
     gene            2505..4688
                     /locus_tag="VFR82_15405"
     CDS             2505..4688
                     /locus_tag="VFR82_15405"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEU4503036.1"
                     /translation="MNTARKLQKPPESQRHQTRSSRLSLFEIGRNCWRVERADRASFL
                     VDGEAYFRAFREAAIHARHHIIILGWDFDSRINMLIDREPDGFPDRLGEFLHDLLIRR
                     KDLHIYVLTWDFHMIYWKEREWWLPSKLVAHRRLHFFKDGAHPVGASHHQKIVVIDDS
                     LAFVGGMDFAQCRWDTNRHAAHHPKRQLLQNGGPCRPFHDVQLMVKGPVAAALGNLAR
                     DRWRFATGRFIPPAKTARVEDLWPQGAEPDLSAVEVAIARTAPPFADRAPIGEVEALF
                     LDSLTRAKRYIYIETQYLTSRTIAECLASRLRDPKGPEIVMVLHPNSDGWLEQHTMDV
                     LRGRVLQRLRAADKFHRLNLNYPKIPDLHGQCISMHSKVCIIDDEMVRVGSANLSNRS
                     MGFDTECDLAIEAAGDPAVQHAIARFRHTLLGEHLGISRDDVQRELDKDGSLIGAVER
                     LRGGARSLGHFDGTVSADVNEVVPDEAFIDPSRPYDAQFFPVERRKPAVRQIVAGTAG
                     LFLLVLLAGLWHWTPLRDHIEVSSLVNYLEDFANGPLALPITMTGFLIGGLLVMPVMI
                     LIAVTILAFGPWWGFWYALIGMTASAMLTFWMGRLLGRRVVDYLPGSRVHRISRALAA
                     KGVLTVVALRIIPVAPFSILNAMAGATHIRTRDFFVGTVLGEMPGLVGLALFLDQVTE
                     TIRHPGWKSILILVGIACLMVFVAWGLSRWLSSRSTEGDHGKSAA"
     gene            4691..5389
                     /locus_tag="VFR82_15410"
     CDS             4691..5389
                     /locus_tag="VFR82_15410"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU4503037.1"
                     /translation="MRLTFATYNIHRCVGRDGRYDPDRIRDVIRELDADVIAVQEIDA
                     PDRHGLELAHWLGEESKLQVIAGPTLLERKGHHGNAVFTRCQRGEIRLLDFSVPMYEP
                     RGAIDLDLSCGGVTVQVIATHLGLRPWERRIQVERLLQGVGENCVLMGDFNEWWLWGH
                     PLRAIKAIFGHSPALPTFPSFRPVLALDRIWVRRTGRVKSLAVHRTRLSAKASDHLPL
                     KAVVEWDAEENTDR"
     gene            complement(5408..>5674)
                     /locus_tag="VFR82_15415"
     CDS             complement(5408..>5674)
                     /locus_tag="VFR82_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503038.1"
                     /translation="KEGTLHLVSGTLLKLDLLNGRGLLKTDLGRPIYFEVPKAYLFEN
                     VIVGARIALRLDEEGRAVRVMDTAIADVVITPDQPLAATAPEDQ"
BASE COUNT         1073 a         1642 c         1665 g         1294 t
ORIGIN      
        1 gggcgagttc gcaaggactt tcttgcccga tgcctcggga gcaaatgcgc gacagctgtg
       61 tgaggcggaa taattgtctc ctcagcggac aactcgtcgg tgagtgacgg taagaggacg
      121 gatattttga tgagtgggcg cccgctcagg aacggaatag gtaggcagag gagatgaacc
      181 cctctgcctt tgccgtcttt cgccgtattc gacgaagccg cgggtttgtc acgaaatcga
      241 gagtgggcga ctctcaggct caatgacgag cgatccgaaa cgggggcgcg cccggatcgt
      301 tcatgtcccg tcggtttcgt catcgacgga agacaacgcc tttcttcatc acacggagaa
      361 tccctctcgc acggcataca gtcgtgctcg accggcctca agccgcgagg gaggattgcg
      421 acgtcagatc gtccttccgt tgctgcatcg tcatgccaag cgcgagcacg tctaactgtg
      481 ccgcggcgag cggtaggatc gcgtcctcct cttccgcaac gtgctcgatc acggcatcct
      541 ccagctcagt gaacatctct cggtagtcgg tgctcttcgg atccagagat tgcaattgct
      601 gaatcagctc tctgaccgtc ccgtgactgt catacgcggc ggtgataatt tccgactccg
      661 cttcttcatc cgtctcgtcc tccaacacct cgagttcggc tccgtcgatg tcatccattg
      721 cgccttccgc ttcttgatcc ctctcgccgt catcggagac atccaatccg ttgccctctg
      781 tctcatagtc aagcgattcg agcttggcct ggacggcggg gtacagaagt tcttcttcaa
      841 gcttcgaatg gatttcgagc tcagtgaaca accgccgggc gatcgaaccc ttgtcgtcgt
      901 ccgaagcgcc atgaaactcc tcaaagagct ttcgcacttg tttatgatcc gccttgagta
      961 attgcactgc atcaagcggc tgggaactct tggacttctt cggcgtccgc tttgccatag
     1021 cttcctcctt gccgttcacg tgaagcccct ccggggcgtt acccattcac gaccgtccca
     1081 ccattgggat gcagtacttg accggtcgtg taagatgaat cctgagacgc aagaaacaca
     1141 tagcttgggg cgacctcctc cggttggccg gcgcgtttga gaggcacatc ggaaccgaat
     1201 gatgctacct tgtcagccgg gaaggtggaa ggaatgagag gagtccaaat tgggcccggt
     1261 gccacagcgt tgacgcggat ctttttctca gccagggctt gcgatagcga acgggtaaac
     1321 gcaacgatcg cacccttggt cgaagcatag tcgagcagtt gcgcgctacc tttataggcg
     1381 gtgaccgacg tcgtattgat gatggcgctc ccttccttga gatggggcag cgccgccttc
     1441 cccatataaa agtacgagaa gatatttgtg cgaaacgtgc gctccaactg ctccgcggtg
     1501 atatcctcca aactgtcctg ggggtgttgc tccgccgcat tgttcaccag tatgtcgagt
     1561 ctgccgaatt cacggaccgt ttgttggacg gtctgtacgc agaagttctc gtctccgaca
     1621 tcgcccgcaa tcacgagaca gcgccggccc tcaccttcca ccatgcgcct ggtctcctcg
     1681 gcatcctcgt gctcgttcaa atagacaatc gtgacattgg cgccctcttt tgcataggcg
     1741 atcgccactg cacgaccaat tccgctatcg ccgccggtga tgagcgccac cttatcgcgc
     1801 agctttccgc atccgcgttg ctcggggctc tcgctttccg gccgcggcgt catcgctgtt
     1861 tcgagcccgg gctgacgcga ctggtgttga ggggggcgct gctgtgtttt tgtgtccggc
     1921 atgtgctctc gttatctccc tgtggttccg gttgatccgt tgttgttggt tcctgcgttg
     1981 ctcccgctgt gagtctgtcc gccgattccg gaatgaccgc tgcctgcact gcccattccg
     2041 gagccgctgc ccggtccagg gcttgctccg ctgccggttc caccttgacc ggtcttccct
     2101 gggcctcggt gtgtgccagg ccggaattta tttttggatt gtccagaagg tgacggagag
     2161 gccgattggg cgatggcgct taacggggcc agccccatca ccactatcaa gccccaaaca
     2221 acgagcagcg accccaggcg caaaggcatt ccacatctcc tcagagaaca ttgaggcggc
     2281 tgcaagaggc agacccaggc gttccgtgcg cgcaaggtgg tattgcctcg cgagggtgga
     2341 ggacgacagc atcctatctg cgctcagggg gacaaagtgt ctgacgtagg acagctggtc
     2401 ccttcgctga gggggtggcg cattcaactc tctcgaagac gctgtcattt accagtctca
     2461 cacgagcaaa ggcattcgac ttgcagttct gcactgaccg ccatatgaat accgcgagaa
     2521 aattgcagaa gcctccggaa tctcagcgtc atcagacgcg ctcctctcga ttatctcttt
     2581 tcgaaatcgg ccggaattgc tggagggtcg aacgcgctga tcgtgcctca ttcttggtcg
     2641 atggcgaggc atattttcgg gccttccgcg aggctgccat ccatgcacgc catcacatca
     2701 tcatcctggg ctgggatttc gacagccgaa tcaacatgct gatcgatcgt gagccggacg
     2761 gtttcccgga ccgcctcgga gagttcctgc acgatctgct tattcgcagg aaagatctcc
     2821 atatctatgt gctgacctgg gactttcaca tgatctactg gaaggaacgg gagtggtggc
     2881 tcccttccaa actggtggca caccgacggc tacatttctt taaagacgga gcgcatccag
     2941 tgggcgcgtc ccatcatcag aagattgtgg tcatcgatga ttcgttggcc tttgtgggcg
     3001 gaatggattt cgcacaatgc cgctgggata cgaaccgtca tgccgcccat catcccaagc
     3061 gtcagctctt gcaaaatggg gggccatgcc ggccatttca cgacgttcag ctcatggtca
     3121 aggggcccgt cgcggccgcg ctcggaaacc tggctcgtga tcgttggcgc ttcgcgaccg
     3181 gccgcttcat tcctccggcg aagacggcgc gtgtggagga tctctggcct cagggcgcgg
     3241 aacccgattt gtccgctgtt gaggttgcca ttgctcgaac ggcgccgccc ttcgcggatc
     3301 gtgcgccgat tggggaggtt gaagcactgt ttttagacag tctgactagg gcgaagcggt
     3361 atatctacat tgagacccag tatctgacgt cacgcacgat agccgagtgc cttgcctcgc
     3421 ggcttcgaga tccgaaaggg ccggaaatcg tcatggtact gcatccgaat agtgatggct
     3481 ggcttgagca acacacgatg gacgtcctca ggggaagagt gttacagcga cttcgggccg
     3541 cggacaagtt tcaccgcctg aatctcaact atcctaaaat tcccgatctc cacggacaat
     3601 gcatcagcat gcacagcaaa gtttgcatca tcgacgatga gatggtccgt gtcgggtcgg
     3661 caaatctcag taatcgctcc atggggttcg ataccgaatg cgatctggcg attgaagcgg
     3721 ctggggatcc ggccgttcag catgccatcg cgagattccg gcatacctta ctgggggagc
     3781 atctaggcat tagccgggac gacgttcaac gagaacttga caaggatggt tcgttgatcg
     3841 gagcggtcga acggctgcgc ggtggagcgc ggtctctcgg acattttgac gggacggtgt
     3901 cggccgacgt gaacgaggtc gtgcccgacg aagcgtttat cgaccctagc cggccctacg
     3961 atgcccaatt tttccccgtg gaacggcgca agccggctgt tcgacagatc gtcgcaggca
     4021 cggccggatt gttcctgctg gtcttgctcg cgggcttgtg gcactggacg cccttgcgcg
     4081 atcacatcga agtctcctcc ctcgtcaatt atttggagga ctttgccaat gggccattgg
     4141 ctttgcccat cacgatgacg gggttcttga tcggcggact tctcgtcatg ccggtcatga
     4201 ttttgattgc ggtgaccatc ctggctttcg ggccttggtg gggattctgg tatgcgttga
     4261 tcggcatgac ggcaagtgcc atgttgacgt tctggatggg ccggctgctc gggcgacggg
     4321 tagtggatta cctccccggg tcccgcgtgc atcgcatcag ccgcgcactg gcggcgaagg
     4381 gtgtcctgac cgttgtcgcg ctgcgcatta tccccgtcgc gccattttcg attctcaatg
     4441 cgatggccgg cgctacgcat atcaggacac gggatttttt cgtgggaacc gtacttggtg
     4501 aaatgccggg tttggtaggc ctcgccctct tccttgatca agtgacggaa accattcgtc
     4561 accccgggtg gaaaagcatc ctcatcttgg tgggaattgc ctgtctcatg gtgtttgtcg
     4621 cgtgggggct cagccgctgg ttgtcgagcc gatcgacgga aggagaccac ggaaagtcag
     4681 cggcataacg atgcgcctca cgttcgccac ttacaacatt caccgatgtg tcggccggga
     4741 tggacggtat gaccctgacc gcattcgtga tgtcatccgc gagctcgatg cagatgtcat
     4801 tgccgtacaa gagatcgacg cccccgaccg gcacggcctt gaactggctc actggttagg
     4861 cgaagagtcc aaactgcaag tgatcgctgg gcccacgctc ttggaacgca aagggcacca
     4921 cgggaatgcg gtgttcacgc gctgccagcg gggagaaatt cggctgctgg acttcagcgt
     4981 gccgatgtac gaaccgcgtg gtgctatcga cctggacctt tcgtgcggag gcgtgacggt
     5041 acaagtgatt gcaacccatc tcggcttgcg gccatgggaa cgacgcattc aggtagagcg
     5101 gctgcttcaa ggggtcgggg agaattgcgt tcttatgggg gatttcaacg aatggtggct
     5161 atgggggcat cccttgcggg ccatcaaagc catcttcggt cattcacctg ccttgccaac
     5221 ctttccctcc tttcggccgg tcctcgcgct cgaccggatc tgggtccgtc gtacgggacg
     5281 agtgaaatcg ctggcggttc atcggacgcg gctatcggcc aaggcctcgg accatctgcc
     5341 tctcaaggcc gtcgtggaat gggacgccga ggaaaacacc gaccgataac cagatgctct
     5401 gcggtcgtca ttgatcttct ggtgcggtgg cagccagggg ttgatctggt gtgatgacga
     5461 catcggcaat ggccgtgtcc atgaccctga ccgcccggcc ttcctcatcg agtcgcaggg
     5521 cgatgcgagc gccgacgatg acgttctcga acaaatacgc cttgggcacc tcgaaataaa
     5581 taggacgccc caagtctgtc ttcagtaatc ctcgcccgtt caacagatcg agcttcaaca
     5641 gggtgccgga aacaagatgc agcgtgcctt cctt
//
LOCUS       DASTQY010000481         4793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1727869, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000481 DASTQY010000000
VERSION     DASTQY010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727869"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..525
                     /locus_tag="VFR82_15420"
     CDS             130..525
                     /locus_tag="VFR82_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503039.1"
                     /translation="MPTTTVTTPSASDILFHRAGSLTSTEPLKHKPEAPRMVRLTVNL
                     PSHLVEQMRDAVYWTPGLTLAWLVARAVRASLTELETIHQGPFAKRTKPLRAGRPRLL
                     GQSMKVRPLGGDDSTGTPPQGAVSLRPIP"
     gene            complement(576..1340)
                     /locus_tag="VFR82_15425"
     CDS             complement(576..1340)
                     /locus_tag="VFR82_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEU4503040.1"
                     /translation="MSTALLTTVAEATTQAVGKLDVDRLHREYWDQNEFVVIPQFLPR
                     DFVEATLVPQAQRVKGQLNRNYIPGHKKGGSVSYYTVQEHAPLFLDLYRSPDFLAFLS
                     RLTEARLMLCPDNDPHSCALYYYTEAGDHIGFHYDTSYYKGARYTILMGLVDKSTQCK
                     LVCELFKDVPNRTPRTLELVTQPGDMVIFNGDKLWHAVTPLGAGEERIALTMEYVTNP
                     EMGRFKRLYSNLKDSFAYFGIRGVFKGICVSSGRPR"
     gene            complement(1385..2080)
                     /locus_tag="VFR82_15430"
     CDS             complement(1385..2080)
                     /locus_tag="VFR82_15430"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEU4503041.1"
                     /translation="MCSAVLLTSSVLVTASAQDVTDDQSRKEVREVVKRLQSRYEKTR
                     DLQADFVQKTTIEGFERPMTSSGKVYIKKPGRLRWNYLEPSTEDIYVDRDDVKVYVPE
                     HKQVLVGKLTQMAASKAPLELLQGAAKLDASFESEPTAGNGRGIGGVRLVTLVPKAHE
                     GDAHRTVQKIVVEVFPKTYFIRSLWLYESSGNVSNFEFSALQSNIGLGDDLFVPKLPP
                     DVEVVKAPVLGQP"
     gene            complement(2095..4518)
                     /locus_tag="VFR82_15435"
     CDS             complement(2095..4518)
                     /locus_tag="VFR82_15435"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU4503042.1"
                     /translation="MGVTSSAKRGETRRAPAPPSHLKREVIGVMLIALSLLTLLSLLS
                     FVPGEPKVVATPAATMTPPTHNLIGSVGAVFSSLLFSLIGGAAYLFPILLGLLGGRCF
                     TQSDLSIRLRNAGASLAALLFLSGLLHLEVTAVPTVSSGMVFRGQAGGLFGQLLAEGL
                     RAYFANTGAHILIISGFLVALLFTAPLSLAQLAVQAPQWGRNAFARVNALMPKRKQDA
                     GVPDAAKPTKTRKPKSLRAVIEEVLPTPEDNPSAEVFSVTPPTVVLPEPREVVEPEPD
                     APLVGKVEDTGDYQLPDADELLTEPSGVLARITDEELKGQSDILSKALLSFGIEGRVT
                     EVRPGPVVTMYEFEPAPGTKVARIVNLADDLALAMKAISLRIVAPIPGKSVVGIEVPN
                     SQRETVSMKEVVASDAFTRARSKLTLALGKDIFGTPVTADLKTMPHLLVAGATGAGKS
                     VSLNTMLISILLSAKPNEVKLLLIDPKMLEFQTYDGIPHLLRPVITDPKSAARGLSWV
                     VQEMERRYKLLAEAGVRNIDSYNRKVAGAQGVLGDAGTEVKPDQPELPIRFLSEEERL
                     AAGETALPDGSPGSFMPPPTPPEPLPYIVVMIDELADLMMVAPKDVEDKIARLAQMAR
                     ASGIHLVLATQRPSVDVLTGLIKANFPARIAFQVSSKTDSRTILDANGAEALLGRGDM
                     LYLASGTGRLMRLHGSFVSDDDVRRVVDFVKAQALPSYNQELQSLKQEEAKEEEAQDE
                     VYEQAKELVLSTGQASASLIQRRLRVGYPRAARMIEQMEADGLVGAAGRDGRREVIGR
                     RGPVGDAHE"
     gene            complement(4581..>4793)
                     /locus_tag="VFR82_15440"
     CDS             complement(4581..>4793)
                     /locus_tag="VFR82_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503043.1"
                     /translation="EEPAFAAVVKAAFAHRRKTLVNSLRDEGYDPQAVIDAISAVGIV
                     STVRAETLSLDTFSRLATRLDRPKPS"
BASE COUNT         1007 a         1448 c         1369 g          969 t
ORIGIN      
        1 cacactgtcg ccccgattct cggccaacaa ggtcgcgtgc caatctccga cggcacgcga
       61 cctttcagtg cggatcttca acgacgccgt tttgggcgat tcacattcat catgacaagg
      121 aggaagacga tgccaaccac cacggtcacg accccaagtg cctccgacat cctgtttcac
      181 cgtgcaggat ccctcaccag caccgaaccg ctgaagcaca agccggaagc gccacgcatg
      241 gtccggttga ccgtcaatct gccgtcgcac ctagtcgaac aaatgcgcga cgcggtgtac
      301 tggacaccgg gacttaccct cgcttggctc gtggcaagag cagtgcgcgc ctcactgacg
      361 gaactggaga ccattcatca aggacctttt gccaaacgta caaagccgct ccgggccggc
      421 cggcccagac tgttgggcca atccatgaaa gtccggccgc ttggaggcga tgactctaca
      481 ggcaccccac cccagggggc tgtttcgtta cgaccaatac cctgagcgag cggcgcgagg
      541 actcgaggtg gatggaaggc gagccaatag caggatcagc gaggtctgcc ggaactcacg
      601 cagattccct tgaagactcc gcggatgccg aaataggcaa acgagtcttt gagattggaa
      661 tagagccgct tgaagcggcc catctccgga ttggtcacgt actccatggt caaggcaatc
      721 cgttcctctc cagctcccag cggggtgacg gcgtgccaga gcttgtcacc gttgaaaata
      781 accatgtcgc cgggttgcgt gaccagttcg agcgtgcgag gcgtgcggtt gggaacatcc
      841 ttgaagagtt cgcacaccag tttgcactga gtggacttgt cgacgagccc catcagaatc
      901 gtgtagcggg cgcccttgta atagctggtg tcataatgaa atccaatatg atcgccggct
      961 tccgtgtaat agtacagcgc gcatgaatgt ggatcgttgt caggacacag catgagcctg
     1021 gcttcggtaa gccggctgag aaatgcgagg aagtcgggcg agcggtacaa gtcgagaaag
     1081 agcggcgcgt gctcctgcac cgtgtaatag ctgacgctgc cgcccttttt atggcccggg
     1141 atataattcc ggttcagctg tcccttgacg cgctgggcct gcggaaccaa cgtcgcctcg
     1201 acgaagtccc tcggaagaaa ttgtggaatg acgacgaatt cattttggtc ccagtattcg
     1261 cggtgcaatc gatcaacgtc cagcttcccg accgcttgtg ttgtggcttc ggccaccgtc
     1321 gtcagcagtg ctgtactcat cggagacatt cccttctcag ctcgcggctg tcgaccgcca
     1381 cgaatcaggg ctggccgagt accggagcct tgacgacttc gacatcgggc gggagcttcg
     1441 gcacaaaaag atcgtcaccg agcccgatgt tcgactgcag agcggaaaat tcgaaattag
     1501 acacattgcc gcttgactca tagagccaca ggcttcggat aaaataggtc ttggggaaca
     1561 cttcgacgac gattttctgc acggtccgat gcgcatcacc ttcatgcgcc ttcggcacca
     1621 aggtcactag cctgaccccg ccaatcccac ggccgttacc cgctgtcggc tcggactcga
     1681 atgaggcgtc cagttttgcg gcgccttgta agagctcaag cggcgccttt gacgccgcca
     1741 tttgcgtcag ctttcccacc aggacttgtt tgtgctcggg aacatacact ttcacatcat
     1801 ccctatccac atagatgtcc tcggtcgaag gctccagata gttccatcgc aagcggccgg
     1861 gcttcttaat gtagactttg cccgatgagg tcatcggtcg ttcgaatccc tcaatagtcg
     1921 tcttttgcac gaaatccgcc tgaagatctc tggtcttctc atagcgcgat tgcaagcgtt
     1981 tcaccacttc ccgcacttct ttgcgagact ggtcatccgt cacgtcttga gccgacgccg
     2041 tcaccaagac gctgctcgtc agcaagacgg cgctacacat gcaaccccac cggatcattc
     2101 gtgggcgtct ccaacagggc cgcgccgccc gatgacttca cgacggccgt cgcgtcccgc
     2161 tgctcccacc agaccgtccg cttccatctg ctcgatcatt cttgcggcgc gcggatagcc
     2221 gacccgcaag cggcgctgga tcagtgaggc tgaagcttgc cccgtcgaga gaaccaactc
     2281 tttggcctgt tcatagactt cgtcctgtgc ttcttcctcc ttcgcctctt cctgcttcaa
     2341 ggactgcaat tcctgattgt aactgggcaa cgcttgtgct ttgacgaagt cgacgacgcg
     2401 gcgcacatcg tcgtcggaga cgaacgagcc gtgcagacgc atcagacggc cggttccgga
     2461 tgccaggtag agcatatcgc ctcgaccaag taacgcctca gcgccgttgg cgtcgagaat
     2521 cgtccgcgag tccgttttgg acgagacttg aaacgcgatg cgggcaggaa agttggcctt
     2581 gatcaagccg gtcagcacat ccaccgacgg tcgctgtgtg gccagcacca agtgaatgcc
     2641 cgaagccctc gccatctgcg cgaggcgggc aattttgtcc tcgacatctt tcggcgcgac
     2701 catcatcaaa tccgcaagct catcaatcat gaccacgata tagggtaacg gctccggagg
     2761 cgtcggagga ggcatgaagg aaccgggact tccgtccggc agggccgtct cgccggccgc
     2821 caggcgctcc tcttcagaga gaaaccggat cgggagctcg ggctggtctg gctttacttc
     2881 cgtaccggcg tcacccagca cgccctgagc gccggcgact tttcggttat aggaatcgat
     2941 gttgcgcaca ccggcctctg ccagcagctt gtagcgacgc tccatttcct gaacgaccca
     3001 gctcagtccc cgcgccgccg atttgggatc ggtaatgacg ggtcgcagga gatgaggaat
     3061 cccatcgtat gtttggaact ccagcatctt aggatcgata agcagcagct tcacttcgtt
     3121 cggttttgcc gacagcaaga tgctgatcag catcgtgttc aagctgacgc tcttgcccgc
     3181 accggtcgcg ccggcgacaa gcaaatgcgg catcgtcttg aggtccgccg tgaccggagt
     3241 cccgaaaata tccttgccaa gagcgagggt gagtttcgag cgcgctcttg tgaaggcgtc
     3301 gctggcgacg acttccttca tggagaccgt ttcgcgctgc gagttgggca cttcgatgcc
     3361 gacgacggat tttcccggaa tcggggcgac aatgcgcaaa ctgatcgctt tcatggctaa
     3421 cgccaaatcg tccgcaagat tcacaatcct ggccaccttg gtaccgggcg ccggctcgaa
     3481 ctcatacatc gtgacgacgg gacccgggcg cacctcggtg actctgcctt caatgccgaa
     3541 actcagcaac gctttcgaca aaatgtccga ctgccccttc aactcctcgt cggtgatgcg
     3601 ggcgagtacc cctgagggtt ccgtcaataa ttcatccgca tccggcaatt gatagtcgcc
     3661 ggtgtcctcg actttgccga cgaggggcgc atccggctcc ggttcaacca cttcacgcgg
     3721 ctcaggcaaa acgaccgttg gcggcgtcac ggaaaacacc tcggcggaag ggttgtcttc
     3781 gggtgtgggg agaacttctt caatgacagc acgaagtgac tttggctttc gcgtcttggt
     3841 cggcttcgct gcatccggca cgcccgcgtc ctgcttccgc ttcggcatca gcgcattcac
     3901 tcgtgcaaac gcgtttcgtc cccactgtgg cgcttgcacg gcgagctgag ccagcgacaa
     3961 aggcgcggtg aagagcagcg cgaccagaaa gccggagatg atgagaatat gagcgccggt
     4021 attcgcaaaa taggcacgca atccctcggc cagcagttgt ccgaagaggc ctcccgcctg
     4081 gcctcgaaag accattccgc ttgagactgt cggcaccgcc gtcacttcca agtgaagcaa
     4141 accgctcagg aacaacagag cagccagtga ggctccggcg ttacgtagtc tgattgaaag
     4201 atcgctctgg gtaaaacacc ggcccccgag cagtcccaac aggatgggga acagataggc
     4261 ggcaccgccg atgagagaga acaacagaga ggaaaagacc gcacccacgg agccaatcag
     4321 gttgtgagtc ggtggggtca tcgtcgctgc aggggtggcg acgaccttcg gctcacccgg
     4381 cacaaaggac aggaggctta atagggtgag caggctgagt gcaatcagca tgacgccgat
     4441 cacttcacgc ttgagatggg aagggggggc aggggcgcgg cgggtctcgc cccgcttggc
     4501 cgagctggtt acgcccatgt gccggatgct aacacagggg gtcaaccaat tcaaacgacg
     4561 actgtcatca tgacatgtgt ttatgacggc ttgggacgat cgaggcgtgt ggcaagtcga
     4621 ctgaaagtgt cgagactcaa tgtctctgcc cgaacggtcg agacgatgcc gactgcgctg
     4681 atggcatcta tgaccgcctg tgggtcgtat ccctcatcgc ggagtgagtt caccaaggtc
     4741 tttcgtctat gcgcaaacgc cgccttcacc acagcggcaa aagccggttc ttc
//
LOCUS       DASTQY010000482         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1728038, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000482 DASTQY010000000
VERSION     DASTQY010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728038"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1788)
                     /gene="ettA"
                     /locus_tag="VFR82_15445"
     CDS             complement(106..1788)
                     /gene="ettA"
                     /locus_tag="VFR82_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEU4503044.1"
                     /translation="MATNDKQVIFSLVNVGKVYPPKKQVLREIYLGFYYGAKIGVLGL
                     NGSGKSSLLRIIAGVDQNFTGEISRSKGYSIGLLEQEPQLDPNKTVKEVVEEGKHELV
                     ALLKEYEAVSNKIGDSSPDEMEKLLDKQAQLQEKIEAANGWELEHHLEIAMDALRCPP
                     AEQKIDTLSGGEKRRVALCRLMIQEPDILLLDEPTNHLDAESVQWLEQHLQQYKGTVI
                     AVTHDRYFLDNVAGWILELDRGHGIPFQGNYSSWLEQKQERLAQEEKAESKRQKTLEH
                     ELEWIRMSPKARQSKGKARLNRYEELVSQRQDQRTDDLEIYIPPGPRLGDVVVQAKAV
                     TKGFGDKLLYEDISFDLPKGGIVGVIGPNGAGKTTLFKMIIGKDKPDAGSIKIGETVK
                     LGYVDQDRSLDGNKTVYEVISEGQETIKLGKVEVNARGYCARFNFAGTDQQKKVKDLS
                     GGERNRVHLARMLKEGANLIILDEPTNDLDVNTLRALEEGLENFAGCAVISSHDRWFL
                     DRIATHILAFEGDSKVVWFEGNYSDYEADRKKRLGKEADQPHRIRYRKLTRS"
     gene            complement(1874..2044)
                     /locus_tag="VFR82_15450"
     CDS             complement(1874..2044)
                     /locus_tag="VFR82_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503045.1"
                     /translation="MLTLMHKECGGPALEPNTSVGEVCAIPIDRFPFPCFTCLEEIID
                     ESEVRLSEELGI"
     gene            complement(2049..>2781)
                     /locus_tag="VFR82_15455"
     CDS             complement(2049..>2781)
                     /locus_tag="VFR82_15455"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU4503046.1"
                     /translation="IRGDRLDGADRHIVFITGLLSTRWGNKSRALLQWCEEQGWSFCC
                     YDVRGFGDSDGVFTDYTLSDWLADARAVLSMLRNDAAARKTGEGLTLVGNSLGGWIAW
                     LMAQECEEVDRLILIAPAFNMMGVRAQTISPERRHDWHSTGWMPWDDDPAHRDWPLSW
                     KWVVESEAYWKSSFNRLRHVRTTIVHGLGDTVIHPEGSREFVEHLRRRDSSFPVELLL
                     IAGDHRLSSPDHMEQLRRLLVDGAI"
BASE COUNT          537 a          849 c          729 g          666 t
ORIGIN      
        1 cgcttaccac cccgcgccca tccgcaaacg taacgctcag tagtcttcgc gtcgcgaacc
       61 tcgctgctcg ccttttcaac agccttgtcg tgcgtccgag gcccgttagc tgcgagtgag
      121 ctttcgataa cgaatgcgat gtggctggtc ggcttccttg ccgaggcgtt ttttcctgtc
      181 ggcctcgtag tcgctgtagt tgccttcaaa ccagactacc ttgctgtcgc cttcgaacgc
      241 taagatgtgc gtcgcaatcc ggtcgaggaa ccaccggtcg tggctgctga tgaccgcaca
      301 gcccgcaaaa ttctcgagcc cttcttcgag cgcgcgcaat gtgttcacgt cgaggtcgtt
      361 tgtgggctcg tccaagatga tgaggttagc gccttccttg agcatccgcg cgagatggac
      421 acggttgcgt tcaccgcccg acagatcctt taccttcttc tgctgatcgg tccccgcaaa
      481 attgaaccgc gcacagtaac cgcgcgcgtt aacctcaacc ttcccgagct tgatggtttc
      541 ctgcccctcc gaaatcactt cgtagacggt tttgttgccg tcaagactgc ggtcttggtc
      601 aacgtacccc agcttgaccg tttcgccgat cttgattgaa ccggcatccg gtttgtcctt
      661 cccgatgatc atcttgaata gcgtcgtttt gccagcgccg ttggggccaa tgacgccgac
      721 gatgccacct ttaggcagat cgaagctaat atcttcgtaa agaagtttgt cgccaaagcc
      781 tttggtcaca gctttggctt gcacgacaac atcgcccagc cgcgggcccg gcgggatgta
      841 gatttccaga tcatcggtgc gctgatcttg ccgttgactg actaattctt cgtagcggtt
      901 caggcgggct ttgcccttcg actgccgcgc cttcggtgac attctgatcc attccaactc
      961 gtgctcgagc gtcttttggc gtttcgattc cgccttttct tcctgggcca gccgttcctg
     1021 cttttgctcc aaccatgagg agtaattgcc ttgaaatgga atgccgtggc cgcggtcgag
     1081 ctccaaaatc cagcccgcca cattgtccag gaaatagcga tcgtgcgtca ccgcgatgac
     1141 ggttcccttg tattgctgca ggtgctgttc cagccattga acggactcgg catccagatg
     1201 gttggtgggc tcgtcgagca gcaggatatc cggttcctgg atcatcaacc ggcagagtgc
     1261 cacacgccgc ttttctcctc cggacagggt gtcgatcttt tgctcggcgg ggggacaacg
     1321 aagcgcatcc atggcgatct cgaggtgatg ttccagctcc cagccgttcg cggcctcgat
     1381 cttttcctgc agctgagcct gcttatccag cagcttctcc atctcgtccg gcgatgaatc
     1441 gccgatcttg ttgctgaccg cttcgtattc tttgaggagg gcaaccagtt cgtgcttgcc
     1501 ctcctcaacg acctccttga ccgtcttatt gggatcaagc tgcggctcct gttcgagcaa
     1561 accgatgctg taccctttag atcgagagat ttcgccggtg aagttttgat cgactccggc
     1621 aataatcctc aacagactgc tcttccccga tccgttcaaa cccagcacgc cgatcttggc
     1681 gccgtaatag aagcccagat agatctcacg cagcacttgc ttcttcggtg gatatacctt
     1741 gccgacattg acgagagaaa aaatgacttg cttgtcgttc gtggccatga gttggtcgct
     1801 gctccttctt ctgttcgcga gtccttcgca aaagaggcac cttaccagag gccggagggc
     1861 tccgacaatc ctcctagatg cccagttcct ctgagagacg cacctcggat tcatcgatga
     1921 tttcttcaag acaggtaaag cagggaaagg gaaagcgatc gatcggtatc gcacagacct
     1981 cccctacgga tgtgttcggc tcgagagccg ggccgccgca ttctttgtgc ataagcgtca
     2041 gcatagggtt agatcgcccc gtcaaccaga agccgacgca gctgttccat atggtcaggg
     2101 ctgctgagtc gatggtcgcc ggcgatcaac aggagttcaa ccggaaacga cgaatcgcga
     2161 cggcgcagat gctccacgaa ctctcggctg ccttccgggt gaatcaccgt atctccgagc
     2221 ccgtggacaa tggtagtccg aacgtgcctc agccggttga agctcgattt ccagtaggct
     2281 tcgctctcca ccacccattt ccaggatagt ggccaatcac gatgcgccgg atcatcgtcc
     2341 catggcatcc agccggtact gtgccagtca tggcgccgtt cgggagaaat ggtttgcgct
     2401 cgaactccca tcatgttgaa ggccggtgca atgaggatca atctgtccac ctcttcgcac
     2461 tcttgggcca tcaaccatgc gatccagccg ccgagtgagt tgccgacaag ggtcaacccc
     2521 tcaccggtct tgcgggcagc tgcgtcattc cggagcatcg agagaacggc tcgtgcatcc
     2581 gcgagccagt ccgacagagt gtaatcagta aagaccccgt cggaatcacc gaatccccgg
     2641 acatcgtaac agcaaaagct ccatccttgc tcttcgcacc attgcaacaa ggcccggctt
     2701 ttgttccccc agcgtgttga caacaagccg gtgatgaaca cgatgtggcg gtccgctccg
     2761 tctaagcgat ccccgcgtat c
//
LOCUS       DASTQY010000483         5978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1551948, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000483 DASTQY010000000
VERSION     DASTQY010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551948"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..534)
                     /locus_tag="VFR82_15460"
     CDS             complement(193..534)
                     /locus_tag="VFR82_15460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU4503047.1"
                     /translation="MTILDGIADAVVKLDGQAKFLAMNKAAATIYERFNLDFFRDMKG
                     KSVWELFPDLKGTTVERELRSVMQDHMQISFEFYYPKDQHWYEVKGYPASPGAILVFR
                     DITDKKAASPA"
     gene            complement(1042..1428)
                     /locus_tag="VFR82_15465"
     CDS             complement(1042..1428)
                     /locus_tag="VFR82_15465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4503048.1"
                     /translation="MPMITVLIVDDHAHVRQVLRQIIESYDDLTIAGEALNGEEAVLL
                     AAKLKPAAVVMDVHLPVLNGIPATTLIKVNNPFTAVIGLTAGDPREDEKAMTIAGASI
                     VINKSEVLHALYPAIVNAIKHIKKTD"
     gene            1738..2136
                     /locus_tag="VFR82_15470"
     CDS             1738..2136
                     /locus_tag="VFR82_15470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4503049.1"
                     /translation="MISSEETSAIRVLIVDDETLVRGVLRSILAEHQDVEVVGEAATG
                     DEAVSSIERLQPNVVTLDIRMPRMDGVAAAYEIRSRYPHVKLIGLTELTQGHDYHAMH
                     RAGVVGIYQKSKAAEELYTAIKRAIAGQDS"
     gene            complement(3168..3350)
                     /locus_tag="VFR82_15475"
     CDS             complement(3168..3350)
                     /locus_tag="VFR82_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503050.1"
                     /translation="MGLSFFSFSTEVQNYLHSFENLLAALRRPDISSRFSMEEREVLW
                     HSFAELQRVLVVWDKK"
     gene            complement(3970..4884)
                     /locus_tag="VFR82_15480"
     CDS             complement(3970..4884)
                     /locus_tag="VFR82_15480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU4503051.1"
                     /translation="MDTSKNNGKMTADVHPAKEALRNAQHTEVSAHRATHLTLQQRNR
                     LLERQLAERSRELGRSLVTVRSLTTALDATEQRERSRLAAELHDYLAQLLVLGRMKIG
                     QLQRLSEPSAGDEMVREVDQVLSQALEYCRTLMAELTPSILHEQGLVAGIRALATQMK
                     RHDLEVRVEIDRMAEFRLPTSCTVLLFRSVRELLINAAKHAAVKQATVRLTCDKGLLQ
                     IVVRDESGFDLAACAGPQAVDTGSVRSSKIGLLTIRERMKALNGRFNLSSAPKQGTTA
                     TLTMPISRPTPLCSLHEKRLAADGRKWE"
     gene            complement(5261..>5978)
                     /locus_tag="VFR82_15485"
     CDS             complement(5261..>5978)
                     /locus_tag="VFR82_15485"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEU4503052.1"
                     /translation="ADPECRIDSIAQSWGVISGAADPGRGVRAMAAVDQHLVNRRDKL
                     ILLLTPPFDRTSLDPGYIKGYVPGIRENGGQYTHAAVWTVLAFVALGDGDKAAELFRM
                     LNPIHRVASRSNVQRYKVEPYVVAGDVYAEPPHVGRGGWTWYSGAAGWLYRAGVEWML
                     GFRLRGATLCIDPCIPRHWSGYSIRFRYHSALYNIVVDNPRHVSRGVTSAELDGTPLI
                     GRDNIPLVDAGEHHIRIVLG"
BASE COUNT         1416 a         1590 c         1606 g         1366 t
ORIGIN      
        1 actgtcatta ggaagttatg gaccgatacg gtaatcatcg gcttataggt cgttcatgat
       61 acaccgaagc atttttgaaa gcaggtgccc ttgcggttgt ctctgaggca ggtctggaca
      121 acttgactcg acaattcagc gcgcttgtct caaaaggctg tcagccagaa attggctaag
      181 gttgccgtgg ctctatgctg gtgaagcggc ctttttgtcc gtgatgtccc gaaacaccaa
      241 aattgcaccc ggcgaagcgg ggtagccttt gacttcgtac caatgctgat ctttagggta
      301 gtaaaactcg aaactgatct gcatgtggtc ttgcatcacc gaacgtagtt cccgctcaac
      361 ggtggtgcct ttcaagtctg gaaacaattc ccagacggat ttccctttca tatcccggaa
      421 gaagtccagg ttaaatcgtt cgtagatcgt agctgctgct ttattcatgg ccaaaaactt
      481 agcttgacca tcaagtttca cgacggcatc agcaatgccg tcgagaatcg tcatgaggtc
      541 atctggttga aaagctcgtt gcatgcgctt ccttaaatga acaattaggt tcgaggcgaa
      601 gccaccaacg gaatggagga aggataaaaa aagattatca cgaatcgaac gtaggtgatt
      661 acgatttatc gcgtcacaaa caaaaatgca cgcaatgtaa gcacatgcct acaagttccc
      721 ttgttcctaa caagcatgtg tgcgaatgtg cctcgtcatg gcgtcctctg ttgaccctcg
      781 cgatacttcc gctaatgccc cgtgtctgca tcctcggctc attctcgaaa caactacgca
      841 ggatcgtctc tacctcactg gccattacgt gtgtgaacag tgtgggcagc attttgatcg
      901 acctccaata aagcagccct ccttacaaaa acatgacccc ccgtcgaggc cgaccaccta
      961 atctgacaaa tccggtcaaa gaatttaggt gaggagaacg gaagattgga aagggctgcc
     1021 gcacccttct tttaacgtgc gctagtcagt tttcttgatg tgtttgatcg cattgacaat
     1081 ggccggatac agcgcgtgca gcacttcgct cttgttgatc acaatcgagg cgccagcaat
     1141 cgtcatggct ttttcatctt ctcgaggatc gccagcggtc aaaccaatca cggccgtgaa
     1201 cggattgttc actttgatta atgtggttgc tggtatgcca ttcaatacgg gaaggtgaac
     1261 gtccatgacc accgctgccg gcttgagttt ggccgcaagg agcaccgctt cttcgccgtt
     1321 gagggcctcc cccgcgattg tcagatcgtc ataactctcg attatctggc gcagcacctg
     1381 ccgaacatga gcatggtcat cgacgatgag aacagtaatc ataggcataa gagcttagtg
     1441 ctttgatatg cagtgataaa ttccccggaa gagggattgt ttgccggatg aaacctatga
     1501 attgaaaggt gggccgtatc ttatccgtgg caaagattta atgagtatga gatgagtgag
     1561 ccaaagcttg gatcagcgat cccgcaattg ccaagtgtgt cccatggtcc gataagtcac
     1621 tggtgtgttg caggcattgg cggattgtga tgcgaacggc cttgtaagcg catgcttaca
     1681 taacctaatc agatgttcaa aatcactgtc agctgtgtta cattccgcaa actcatcatg
     1741 atctcatctg aagaaacttc agcgattcgc gtattaatcg tagatgatga gactctggtt
     1801 cgtggtgttc tcaggagcat cctggcagag catcaggacg tagaggtggt tggcgaagcc
     1861 gcgaccggtg acgaggcggt ctccagcatc gaacgactcc aaccaaacgt cgtcactctt
     1921 gatatacgca tgccaaggat ggatggggtt gctgcagcct atgagatcag aagccgctat
     1981 ccccacgtaa agctgatcgg actgaccgaa cttacgcagg gacacgacta ccacgcaatg
     2041 cacagggcgg gagtcgtagg gatatatcag aaatcaaagg cagcagaaga actatatacg
     2101 gctatcaaac gggcgatagc tgggcaggat tcataagggc cgtgcctctc gcgccgatta
     2161 tttgcttcat cgctgcatca tccccttcgt cccaggtaac aatcactacc gcgtaccgat
     2221 tcgcgctccc cacccattag cgttttggat atgggggact ttgggaagcg agatacaaga
     2281 ggccgacagg cgcgggttca acgcgaaaaa ccatactgtg gcggctctta cgaaaagggt
     2341 ccatcctcta cgctatactc aagcgctgtt tgatcagctc gcccagcagt cggcgtgcaa
     2401 tcggtcgcac aatatggagg agcggtgtgc gcggtggatt ttgatgaccc agggaccgcg
     2461 tgggccaaga cgagtttcca ctcacgcagg agtttctggc gtatatgctt ggcgcacgcc
     2521 gagccagcgt cacattctcg cccgtgccgg cctcatttgt tttatccgtg ggaaaatcca
     2581 ggtataggat cgagccagac tcgaagcggc gagttgtgaa tgctatgtcg ttattcaacg
     2641 ggaatatgag cggctcatcg gtagctgttg agttgaagac aggaaatgcc ctcttcgtca
     2701 tcaagtctcc ttcgtagtgc acaagagaaa ggccgacgca gcgaagcttg gtacgtacct
     2761 acacagcgac tggaaacccc tccactgcgg ttcttcaaga caacaactgg ctgaatccat
     2821 ggccaggtac atgactcagc ttggactcga aaagaaggaa gaaggagaag ctgattcctg
     2881 ctctctcgga ctacctcggc caagaagcag gtcagacagc aatgaacggg acgcatagat
     2941 gcagtttcgg agggagagtg atttatctca cgaacccagg gtcgagcagt ttttgggaga
     3001 gacgattcaa tatcccctgt atggcttgtc tctcagcgtc agtcaaatca tcttttttta
     3061 aaagaacttc ggtggcacgt aagtactccc gaacaaattg acggggctca gacataagac
     3121 accctcgcat ggaacggcaa cggagatttc agaaaacgat gtgacgctta cttcttgtcc
     3181 cagacgacaa ggacccgttg gagttcagca aaggagtgcc aaagcacttc ccgttcttcc
     3241 atcgagaaac gggaagaaat gtcgggtcgt cgcagtgctg ccaagagatt ctcgaaggaa
     3301 tggagatagt tctgcacttc agtgctgaac gagaaaaagg ataagcccat ggggccaccg
     3361 cctttctcac aaggtgtcgg cgggtgaccc aaaaggccgt cggcaggatg tgtgggagtt
     3421 atggtgggga gtctgagcta atggggagac ggtgtcaagg cggatcggaa ggattttccg
     3481 tacgtttcgg ctacacagag cttcccgtcg gcagttgtgt aggcaggttg tcgcatctgg
     3541 ccacccccct gaaggccatc ggcatctgtg gatgagagtg ggaaaggaag tctgacgaga
     3601 ttagaggaca gggtcaaggg tgaatctcaa cctgaggggg tactctgact ctgttcgcta
     3661 gcgtacatat aaacaccctc ttctggggga cttcttcttc gcactccgca ggatcttctc
     3721 ctctatggcc ccataccttt caaaaagggg gtcgcaggca cacgacttct ccagttgtga
     3781 ccctgccctt gagaaaccga ccgttttggc agatggcgga ggcgattggc acgaaccacg
     3841 gagtcgtcga tgcacccaca gttaacgcat cgtgttgcca agcagatacg ccctagccca
     3901 tctctcagtt caccgaaatt ttccaggatg agcaggccat ggcagcgttg acatgacaca
     3961 catcctctct cattcccact ttcgcccatc ggctgcaagc cttttctcat gcaaagagca
     4021 taaaggtgtt ggccgggata tcggcatcgt aagggtcgcc gtggttccct gctttggcgc
     4081 cgaggagagg ttaaagcgac cattcaaggc tttcatgcgt tcccggatag tcaggaggcc
     4141 gatcttcgaa gagcgaacac ttcccgtgtc gacagcctgc ggccctgcgc acgccgcaag
     4201 atcgaagccg ctttcgtcac gcaccacgat ctgaagaaga cccttgtcgc aagtcagacg
     4261 gaccgtcgcc tgttttacgg ccgcatgctt tgcggcgttg atcagcagtt cccgcaccga
     4321 gcgaaagagc agcacggtac aactggtagg aagacggaac tcagccattc gatcaatttc
     4381 tactcttacc tctaggtcgt ggcgcttcat ttgtgtcgcg agcgctcgaa tccccgccac
     4441 tagcccttgt tcgtgcagaa tagatggagt taactcggcc atcaacgtcc ggcaatactc
     4501 taaggcttgg ctcaacacct ggtcgacctc tcgaaccatc tcatcgcctg ccgatggctc
     4561 agagagtcgc tggagctgtc cgattttcat acggcccagc acaagcaact gcgccagata
     4621 gtcgtgcaac tctgccgcta gacgtgagcg ttcccgctgc tccgtggcat cgagtgcggt
     4681 cgtcagggac cggacagtca ccagcgaacg acctaactcc cgtgagcgct cggcgagttg
     4741 ccgttctagt aatcggttgc gctgttgcag cgtgagatgt gttgcgcggt gcgcgctgac
     4801 ctcggtgtgc tgagcattgc gtagcgcttc tttcgccgga tggacgtctg ccgtcatctt
     4861 tccattattc tttgaagtat ccatatgcgg ctccctgcgg tcttcagata gaaacacaga
     4921 agtggagacc ctcgtttatg gggggatgtt agacccgcgg gcggaagccg aactgtctca
     4981 aactgaccat tccgctggtc tgcctaaaat aatttagttt tttcctctca ataggttttt
     5041 catctctcag acttaaacca atcccggccg tgaggatgac ataactttat aatgaaaaca
     5101 tacagttgca tcacttcatg tcgctccttt gagtggggca tgcttctgga ttgtggcatt
     5161 ggcgtgcagg gctacccgct cagctgaccc agagctacga taagcgagct aatgagctga
     5221 gcctgaatga cttgcggcga gtgattacct ctcccataca tcatcccagc acaatgcgaa
     5281 tgtggtgctc gccggcgtca acgaggggga tattgtcgcg acctatcaac ggcgtgccat
     5341 ccagttcggc tgatgtcaca ccgcgactca catgtcgcgg attatccacc acgatattat
     5401 agagcgccga gtgataccgg aaacgaatgg agtacccgga ccagtggcga ggaatacaag
     5461 gatcgataca gagcgtggca ccgcgtaacc ggaagccaag catccattct acaccggccc
     5521 gatagagcca gccggccgcc ccgctatacc aggtccaccc tccacgcccg acatgcggcg
     5581 gctcagcgta cacgtcgcct gccaccacgt agggctcgac tttgtagcgc tgaacgttcg
     5641 atcgtgaggc aacgcgatga attggattta acatgcggaa tagctcggcg gccttatccc
     5701 catcacccag cgccacgaaa gccaggacgg tccagacggc ggcgtgagtg tactgtccgc
     5761 cgttctcacg gatccccggc acgtatcctt tgatatagcc ggggtcgagg gacgtccggt
     5821 cgaacggcgg cgtcaagagg agaatcagct tgtcgcgccg gttcaccaga tgctgatcca
     5881 ccgccgccat agcgcgcacg ccgcgtcctg ggtctgcggc accactgatg actccccagg
     5941 actgcgcgat ggaatcaatc cggcactccg gatctgcg
//
LOCUS       DASTQY010000484         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_720276, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000484 DASTQY010000000
VERSION     DASTQY010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720276"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VFR82_15490"
     CDS             complement(<1..864)
                     /locus_tag="VFR82_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503053.1"
                     /translation="MIKRVILLVVEGLGVGATDDASTYGDTSCHTLAHLAETAGGLSL
                     PTLESLGLGHVTTIQGVRSMAQPVGCFGRLGFKSKGVDSLVGYWEIAGCLSEEEGRTF
                     PNGYSREAIAALEAALGQKIVGGRVASGEEALKEFGARHLSDGTPIVWTDGRQTCHVA
                     AHDSVWSGEVLYQRVRESRKSLKDAWGMRRLVAHPLIGAAGALRFGPGRKDYAGEPPG
                     QTMLDLLNRASQILIGVGKVGDLFGGRGLTRSVATSSWSQAMDEVSGMFKKVPRGLIF
                     AGLDVIGSDAVN"
     gene            complement(966..1289)
                     /locus_tag="VFR82_15495"
     CDS             complement(966..1289)
                     /locus_tag="VFR82_15495"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4503054.1"
                     /translation="MALRRDVRYQVQCPVAFVVDGKPGQGTTFNLLRGGCAIDTDMYA
                     AIDDPISLHITVSSQPTPIAIELGKVRWATRQELGVEFMVVERASKQRLDDFLLVVAK
                     EKLTS"
     gene            complement(1296..1976)
                     /gene="deoC"
                     /locus_tag="VFR82_15500"
     CDS             complement(1296..1976)
                     /gene="deoC"
                     /locus_tag="VFR82_15500"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU4503055.1"
                     /translation="MNQSGWDLPSLIDHTILRPEATRADVLRLCREAKEHGFRVIFIP
                     PCYAAEAVAAVSGSPVRVGIPIGFPLGGHATGTKVAEAVEGVRQGASVLDMVINVSRL
                     KSGDHDAVRQDIAEVVLATPQAEHKVILETCYLSDQEKMTACRLAVEAGADYVKTSTG
                     FGARGTTVSDVKLMIAAVGGKAKVKASGGIRDWNMTRQLLEAGADRIGTSASLIILDE
                     WSAARATG"
     gene            complement(2060..>2554)
                     /gene="mltG"
                     /locus_tag="VFR82_15505"
     CDS             complement(2060..>2554)
                     /gene="mltG"
                     /locus_tag="VFR82_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU4503056.1"
                     /translation="LSTMVEHLRQVYTAELHARAQELKMSQHEVLTLASVIEKESASN
                     GEREEISAVFHNRLKKHIPLQSDPTVIYGLPNYDGNIHKKDLLSPSPYNTYRISGLPP
                     GPIANPGIQSIRATLYPSDSRALFFVSKNDGTHQFSTTLAEHNQAVEKYQKKPFRRAG
                     HPRT"
BASE COUNT          523 a          809 c          674 g          548 t
ORIGIN      
        1 gttcacggcg tccgaaccga tgacgtcgag tccggcaaag atcaacccgc gcgggacttt
       61 cttgaacatg ccgctgactt catccatagc ctgactccag gaggaggtgg cgaccgatct
      121 ggtcaatccc ctgccgccga acaaatcacc cacctttccc accccgatca aaatttgact
      181 cgcccgattc aagagatcca gcatcgtttg gcccggcggc tcgccggcgt agtccttccg
      241 gcccggtccg aatcgcagcg ccccggcagc tccgatgagc gggtgtgcga caagacgccg
      301 cattccccat gcgtctttca gcgattttct ggattctcga acccgttgat ataacacctc
      361 accgctccac acgctgtcat gcgcggctac atgacaagtc tgacgcccgt ccgtccagac
      421 gatcggcgtt ccgtccgaga gatggcgtgc accgaactct ttcagtgcct cctcaccgga
      481 cgcgacacgt ccgcccacga tcttctgtcc caatgccgct tccaacgctg cgatggcctc
      541 cctcgaatag ccgtttggaa acgttcgccc ctcttcctcg gagagacatc cggcaatctc
      601 ccaatagccg accagtgagt ctactccctt gctcttgaat cccagacggc cgaaacaacc
      661 aaccggttgc gccatggatc ggacgccctg aatagtggtc acatggccaa gcccgagcga
      721 ttcgagcgtc ggcagactga gaccccccgc ggtctcggcc aagtgagcca gcgtgtggca
      781 ggaggtatcg ccgtacgtgg acgcgtcgtc cgtcgctccg acgccaaggc cctcgaccac
      841 aagcagaatc acgcgtttga tcatgggcgc actataacag atggggctcg agccgcaaat
      901 cgtctcatga ttgaagcaat cacaggcggg atgacgaggc tgttagcgcg acgggaatgc
      961 tctcatcagg aggttaactt ttctttcgcg acgactaaga gaaaatcgtc cagccgctgc
     1021 tttgacgccc gttcgacgac catgaactcc acgcccaact cctgccgcgt ggcccaccga
     1081 accttgccga gttcgatggc gatcggtgtc ggttgggacg aaaccgtgat atgcaggcta
     1141 attggatcgt cgatggccgc atacatgtcc gtgtcaatgg cacacccgcc ccgcaaaagg
     1201 ttgaacgtcg tgccttgtcc tggtttccca tccaccacga acgccacggg acattgcacc
     1261 tgatagcgga catccctacg caaggccatg actcgtcacc cggtcgctcg tgcggccgac
     1321 cattcatcga gaatgatcag gctcgcactc gtgccgatgc ggtcggctcc ggcttccaga
     1381 agctgccggg tcatgttcca atcgcgaatg ccaccggatg ccttgacctt ggccttgccc
     1441 cccacggccg caatcatcag tttgacgtcg gacacggtcg tccctctcgc gccaaatccg
     1501 gtcgaggttt tcacatagtc ggcgccggct tccactgcca gtcggcaagc agtcattttc
     1561 tcttgatcac tgagatagca ggtttcaagg atgaccttgt gctcagcctg gggagtcgcc
     1621 agcaccacct cggcgatgtc ttgccggacg gcatcatgat cgccggattt caagcgactg
     1681 acattgatca ccatgtccaa cacggatgcg ccctgccgca ctccttccac cgcttcggca
     1741 accttggtac cggtcgcatg tccgcctagt gggaatccga tggggatgcc cacacggacc
     1801 ggtgagcccg aaacggcagc caccgcttcg gccgcataac agggtggaat gaaaatcact
     1861 cgaaagccgt gctcttttgc ctcccggcag agacgcagga catcggctct tgtcgcttcc
     1921 ggccgcaaaa tggtgtgatc gatcaacgaa ggaagatccc aaccgctctg gttcatgctg
     1981 tgatttggct ccaggtaatt gatgaaaccc tcaatgatct caccagcacc acttactgct
     2041 agactttggc atcaatctct catgtgcgcg gatgccctgc ccgtctaaaa ggtttcttct
     2101 gatatttttc gaccgcctga ttgtgctcgg cgagtgtggt cgaaaattga tgtgtcccat
     2161 cattctttga cacgaaaaag agcgcccggg aatccgacgg atacagcgtc gcgcggatcg
     2221 attgaatacc ggggttcgcg atggggcctg gaggcaaccc cgagatccgg taggtgttat
     2281 agggactcgg gctcaacaaa tcttttttgt gaatgtttcc atcatagttc ggcaagccat
     2341 agatgacggt cggatcgctc tgcaacggaa tgtgcttctt caatcggtta tggaacaccg
     2401 ccgaaatctc ttcccgttct ccattagatg ctgattcttt ttcgatcacg gatgccagcg
     2461 tcagcacctc atgctggctc attttcaact cctgcgcccg cgcgtgaagc tcggccgtat
     2521 agacctgccg caggtgctcg accatggtcg agag
//
LOCUS       DASTQY010000485         5588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1602122, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000485 DASTQY010000000
VERSION     DASTQY010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602122"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..2752
                     /gene="gyrA"
                     /locus_tag="VFR82_15510"
     CDS             272..2752
                     /gene="gyrA"
                     /locus_tag="VFR82_15510"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU4503057.1"
                     /translation="MPPDERLGQIAIEDEMRSSYLDYAMSVIVGRALPDVRDGLKPVH
                     RRILYGMNEMGLGHNRPYRKSAKIVGEIMGNYHPHGDSAIYDTLVRMAQDFNMRYPLV
                     DGQGNYGSMDGDSPAAMRYTEARMTKLAEEMLADIDKETVDFGPNYDESRQEPLVLPT
                     KVPNLLINGAGGIAVGYATNIPTHNIGEVIDGLLLLLESPEVTIAQLMKKIPGPDFPT
                     AGFIYGMSGIKEAYETGRGLLALRAKVVVETDERTERERLIVTEIPYQVNKAKLIEKI
                     AELIQEDRIKGISDLRDESSDREGVRVVIELKRNEIPLVVLNNLYKHTQLETTFGVIM
                     LALVNNRPEVLNLKQILHHFLEHRREVVVRRTTYELRKAEERAHILEGLKIALDNLDA
                     VIALIRRSQSPDEARTGLMRQFTLSEIQANAILDMRLQRLTQLERAKLVEEYQEVLKQ
                     IEYLKSVLASEALVRSIIKDELAQLREAYRDDRRTQIVKEEAEISLEDLIAEEEVVVT
                     ISHAGYIKRNAASLYRAQRRGGKGRIGMGIKDEDFVETLLTASTHDTLLFFTDAGKVF
                     WLKVHEIPEASRAAKGKALVNLLALAGSEKVTATLAVKDYPADRFVVMATKKGVIKKT
                     ELSAYSNPRQGGIIALGLEDGDRLIGVQVTDGQREILLGTKQGITIRFSENDVRPMGR
                     TAYGVKGITLEEGNEVIGMETITPDSTTAILTVTEGGYGKRTPVGEYRVQGRGGKGII
                     SVKTTERNGLAVGFLQVRDGDEIMLMAAQGKVLRCKVGDIREIGRNTQGVRILDLDGD
                     DDRVVAVARLADSGEREEAGSEEGPAGQ"
     gene            2779..3348
                     /locus_tag="VFR82_15515"
     CDS             2779..3348
                     /locus_tag="VFR82_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503058.1"
                     /translation="MNNPTPQSPPLSPPKKSLDTVVKLALMVFFGSFALIWGGMYLSR
                     PDRSIPPYSVGSQEGTAVSVHVPAWTSDTEIETLIERFRKVGRETRNFGPMKIRPTTP
                     DDPSGRYRRITIFIFTHDAWAEAPILHKYLTGEDQEVREGFKRALRGLYRLTETDEEG
                     RIGPIVDGQDSAATAAYSRQLFKRTLVEP"
     gene            complement(3345..3812)
                     /locus_tag="VFR82_15520"
     CDS             complement(3345..3812)
                     /locus_tag="VFR82_15520"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEU4503059.1"
                     /translation="MRPSGSLDSFSTILSGLAKRLGLQSHLFEVRLQQQWRVIIGEPV
                     GSHTWPVQVRFKKLYLNVRNSVWLQQLMFLKPTLLAKVNEQAGSELITDIAFRVGEIP
                     DARTVVQASSPHQEPVNEAALIEANAHAAAVQDPELRNRLASLMADAFSRPRS"
     gene            3868..4341
                     /gene="smpB"
                     /locus_tag="VFR82_15525"
     CDS             3868..4341
                     /gene="smpB"
                     /locus_tag="VFR82_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU4503060.1"
                     /translation="MTKEKAAEEKVVATNRKAYHDYFIEEKYEAGIMLQGTEVKSLRE
                     GRVNLQDSYASVRGTEVYLHQCHISPYSHGNIMNHDPTRVRRLLLHKVEINKLLGKTQ
                     QKGLTLIPLRIYFTKRGYAKVELGLAKGKKLYDRRESIKAREAGREVQRAIKERK"
     gene            4402..5397
                     /locus_tag="VFR82_15530"
     CDS             4402..5397
                     /locus_tag="VFR82_15530"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU4503061.1"
                     /translation="MSIGHVAATWRQGFILPIAVMTAAFLFACERTPSDRVQGYVEGE
                     FVYIASPFAGALESLSVQRGRQVRSGDALFALERGSEKAARDEAERRLGQALANVEDA
                     KKGKRPSEIDSLKAQLKHAQAALRLSTREVVRQEGLSQVPGAAVELEVDRARAARDQD
                     RQRVAQLQADLDTALLGSRTDQVIAAEAEVHAREAALARADWEFAQKRQQAPKSGLVF
                     DTLYREGEWVPAGRPVVVLLPPENIKVRAFVSETRVGTIRPGDNVQVAVDGVPKPFTG
                     KVSYISPRAEYTPPVIYSQESRDKLVFMVEVVFDPQSAVNLNPGQPVDVRFESTL"
     gene            5394..>5588
                     /locus_tag="VFR82_15535"
     CDS             5394..>5588
                     /locus_tag="VFR82_15535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4503062.1"
                     /translation="MRDEATGQALAIDVHGMTKRFGERTVVDRIGLQVRRGEIFGFLG
                     PNGSGKTTFIRMLCGLLRADE"
BASE COUNT         1277 a         1543 c         1739 g         1029 t
ORIGIN      
        1 gcccttgaag tgcgcaatct ggatgtgtag cgagccaaaa gccaccgacg ggagccagaa
       61 gagccaaccg atcagccgtg gagatgccca aaatgggcat ccggcaggtc gcagcaaggt
      121 ccgcggtccg cgacgcgaag aatcatgaga aacacgtgca aacgggcgcg agttggtaag
      181 cgcccagtat ctcaggggcg acgcgtacgt tgcactctga gcggaacgag caccaggccc
      241 gtgatcatgt tcagcatccc tggagaacga catgccgcct gatgaacgcc taggtcaaat
      301 cgcgatcgaa gacgagatgc gctcctccta tctcgattac gccatgagcg tgatcgtagg
      361 acgcgcactc cccgacgttc gcgacggcct gaagccggtg catcgccgca ttctttacgg
      421 catgaacgag atgggcctcg gccacaaccg gccctaccgc aagtcggcca agatcgtcgg
      481 cgaaatcatg ggcaattatc atccgcacgg tgattcggcg atctacgaca ccctcgttcg
      541 gatggcgcag gatttcaaca tgcgctaccc cttggtggac ggccagggca actacggatc
      601 gatggacggc gattcgcccg cggccatgcg ttacaccgag gcgcgcatga cgaagctggc
      661 cgaagagatg ttggccgaca tcgacaaaga gaccgtcgat ttcggtccca actacgacga
      721 gtcccggcag gaaccgttgg tcctcccgac taaggtgccg aacctgttga tcaacggggc
      781 cggcggcatt gccgtgggct acgctacgaa tatcccgacc cataatattg gcgaagtgat
      841 cgatggcctc ctgctgctgc tcgagagccc tgaggtgact atcgcgcagt taatgaagaa
      901 gatcccggga ccggattttc cgacggctgg cttcatttac ggcatgagcg gcatcaagga
      961 ggcctatgaa acgggtcgcg ggctgctggc tctgcgggcg aaagtcgtcg tcgaaaccga
     1021 tgaacgaacc gagcgtgaac gtctgatcgt caccgagatc ccctatcagg tcaacaaggc
     1081 aaagctgatc gagaaaatcg cggaattgat tcaagaagat cgtatcaagg gcatttccga
     1141 tcttcgcgac gaatcgtcgg atcgcgaggg tgtccgggtc gtcatcgagc tcaagcgcaa
     1201 tgagattccg ctcgtcgtgc tgaacaactt gtacaagcat acgcaactcg aaacgacctt
     1261 cggcgtgatc atgctggcgt tggtcaacaa tcgcccagag gtgctcaacc tcaagcagat
     1321 cctgcatcat tttctcgagc atcgacgcga agtcgtcgtc cgtcgcacca cttatgagct
     1381 gcgcaaggcg gaggaacggg cgcacatcct cgaaggtctg aaaatcgccc tcgacaatct
     1441 cgacgccgtg atcgccctga ttcggcgttc ccagtcgcct gatgaagcgc gcaccggact
     1501 gatgcggcaa tttacgctga gcgagatcca ggcgaacgcc atcctggata tgcgcttgca
     1561 gcggctgacc caactggagc gagcgaaatt ggttgaggaa tatcaggaag tgctgaagca
     1621 aatcgaatat ctgaagtcgg tgttggccag cgaagcgctc gtgcggtcga tcatcaagga
     1681 cgagctggcc cagctgcgcg aagcctatcg cgacgaccgc cgaacgcaaa tcgtcaaaga
     1741 agaagccgaa atcagccttg aggatttaat cgccgaggaa gaagtcgtcg tcacaatttc
     1801 ccatgccggc tacatcaagc ggaatgcagc ctccctctat cgtgcccagc ggcgcggtgg
     1861 caaagggagg atcggcatgg ggattaagga cgaagatttc gtagagacgc tcctcaccgc
     1921 gtcgacgcat gacacgctct tgttcttcac cgacgcgggc aaagtcttct ggctgaaggt
     1981 ccacgagatt cctgaagcca gccgagcagc gaagggtaag gcgctggtca atcttctggc
     2041 tttggccgga agcgaaaagg tcacagcgac cttggcggta aaggactacc ctgctgaccg
     2101 gtttgtggtc atggcgacca agaagggtgt gatcaagaaa accgaactgt ctgcctatag
     2161 caatccccgg caaggcggca tcatcgctct cggattggag gacggcgaca ggctgatcgg
     2221 ggtgcaagtc actgacgggc agcgggaaat tcttttaggc accaagcaag gcatcacgat
     2281 caggttctcc gagaacgatg tacgtccgat gggccgcacg gcttacgggg taaaaggcat
     2341 cacgctggaa gagggcaatg aagtgatcgg catggaaacg attacgcccg attcgacgac
     2401 ggccatcctg accgtgacgg aggggggata tggcaagcgg acgccggttg gcgagtaccg
     2461 cgttcagggc cgcggaggaa agggcatcat cagcgtaaaa accaccgagc gcaacggcct
     2521 cgccgtcgga tttttgcaag tccgggacgg cgatgaaatc atgttgatgg cagcgcaggg
     2581 caaggtgctc cgttgcaaag tcggcgacat ccgagagatt ggccgcaaca cgcagggcgt
     2641 gcggatcctc gatctggacg gagacgacga tcgtgtcgtg gcggtggctc gtctcgcgga
     2701 cagcggcgag cgggaagaag ccggctccga ggaaggcccg gccggccaat aatcgtcggt
     2761 tgttggtcgt cctctccgat gaacaacccc acgccgcaat ctccgccgct ttccccaccg
     2821 aagaaatcgc tcgatacggt cgtgaagctc gccctgatgg tcttcttcgg atcctttgcg
     2881 ctgatctggg gcgggatgta tctcagcaga ccggatcgat ccattccccc ttatagtgtc
     2941 ggctcccagg aaggcactgc cgtctccgtg catgttccgg cctggaccag cgatacggag
     3001 atcgaaacat tgattgaacg ctttcgaaaa gtcggccggg aaacacgaaa cttcggaccc
     3061 atgaagatca ggccgacgac accggacgat ccttcgggac gctacaggcg gattacgatc
     3121 tttatcttca cgcacgacgc gtgggccgag gcaccgatcc tccacaagta cctgacaggt
     3181 gaggaccagg aagtgcgcga gggattcaag cgtgcactac gaggtttgta tcgattgacg
     3241 gaaaccgacg aagagggtcg aatcggtccg atcgtggacg ggcaggattc cgcggcgacg
     3301 gccgcttact ctagacaatt gttcaagcgc acgcttgtcg agccttaaga gcgggggcgg
     3361 gaaaaggcgt cggccattaa cgaggccaac cggttcctca gctcgggatc ttgtaccgcg
     3421 gctgcgtggg cgttggcttc gataagggcg gcttcgttca ccggctcttg gtggggagag
     3481 gaagcctgca cgaccgtccg ggcatccggg atttcaccca cgcgaaaggc gatatccgtg
     3541 atcagttctg atcctgcctg ctcgttcacc ttggcgagca gggtcggctt caagaacatg
     3601 agttgttgca accagaccga gtttcggaca ttcaagtaaa gtttcttgaa ccgaacctgc
     3661 accggccagg tatgggagcc gactggttcg ccgatgatca cacgccattg ttgctgaagg
     3721 cgcacttcaa agagatggga ttggagaccc aagcgctttg ccaaacctga gagaatggtg
     3781 ctgaacgaat caagcgaacc ggaagggcgc atgccgaatg ataggctgcg gctttggacg
     3841 ggtcaaggga gcaatgccga ctgaatcatg accaaggaga aagcagcaga ggaaaaagtc
     3901 gtggcgacca accgcaaagc atatcacgac tattttatcg aagaaaaata cgaagccggc
     3961 attatgttgc agggcaccga ggtgaagtcc ttacgggagg gccgagtgaa cctgcaggat
     4021 agctacgcct cggtccgcgg caccgaagtc tatctgcatc agtgccacat cagtccgtac
     4081 agccatggca acatcatgaa tcacgatccg acgagggtgc gcagactgct tctccataag
     4141 gtggaaatca acaagctgct tggcaagaca cagcagaagg gtctcacgct gatcccgctg
     4201 cgcatttatt tcaccaagcg cggatatgcc aaggtggagc tcggcctggc caaaggcaaa
     4261 aagctctatg accgccgtga atcgatcaaa gcccgtgaag ccggccggga agtgcaacgg
     4321 gccatcaagg aaagaaaata ggagctccgc ctgaggcttg aaacacatcg tgctctagac
     4381 gcaggcggac cccccgggga catgagcatc ggtcatgtcg cggcgacatg gcgtcagggg
     4441 ttcatcctgc cgatcgccgt gatgacggcc gcgtttctgt ttgcctgcga acgcacgcct
     4501 tccgatcgag tccaaggcta tgtcgaaggc gaattcgtct acattgcctc gccgtttgcc
     4561 ggtgcgctgg aatcgttgtc agtccagcga ggtaggcaag tgcgctcggg agacgccctg
     4621 tttgccttgg aacgtggctc ggaaaaagcc gccagggatg aagcggagcg acggcttggc
     4681 caggccctcg cgaatgtcga agatgcaaaa aaagggaagc gcccatctga gattgattca
     4741 ctcaaggcgc aactcaagca tgcgcaggct gccttgcggc tctccacgcg cgaggtcgtg
     4801 cggcaggaag ggttgagtca ggtgcctggt gcggcggtcg agctcgaggt ggatcgagcg
     4861 cgtgcggctc gggaccagga tcggcaacgg gtggcacagc tccaggcaga tctggatacg
     4921 gccttgctcg gctcgcgcac cgatcaagtc attgccgctg aggcggaggt tcacgcccgc
     4981 gaagcggcgc tggctcgagc ggattgggag ttcgcgcaga agcgccagca ggctccgaaa
     5041 tccggactcg tgttcgacac cttgtatcgg gagggcgaat gggtgccggc cgggcgcccc
     5101 gtcgtcgtcc tgctgccgcc cgaaaatatc aaggtacggg cgttcgtgtc ggaaactcga
     5161 gtcggaacga ttcgtcctgg tgacaatgtc caggtcgccg tggatggtgt gcccaagccc
     5221 ttcacgggca aggtcagcta catttccccg cgtgcagagt acacgccgcc ggtcatttac
     5281 agccaagaaa gccgcgacaa attggtgttt atggttgaag tggtgtttga tcctcagagc
     5341 gcagtcaatc taaacccggg ccagccggta gatgtccggt ttgaatctac tctatgagag
     5401 acgaggcgac gggacaggca ctcgcgatcg acgtgcatgg catgaccaaa cggttcggcg
     5461 agcgcacagt cgtcgatcgt atcgggcttc aagtgaggcg cggtgagatt ttcggatttc
     5521 tcggcccgaa tggcagcggc aagaccacat tcattcggat gctctgcggt ctcctgcggg
     5581 cggacgag
//
LOCUS       DASTQY010000486         3411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_121012, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000486 DASTQY010000000
VERSION     DASTQY010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121012"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VFR82_15540"
     CDS             complement(<1..434)
                     /locus_tag="VFR82_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEU4503063.1"
                     /translation="MTDYAVFAQKALNDEILSRQECRALLNAPDDDLLEVLQAAYLVR
                     SRYFGKTVRLQMLQNAKSGACQEDCHYCSQSAVSTAAIERYNLVPRAQMVAGARQAAA
                     SKAQRYCIVISGRSPLDREIDEIAGAVREIKQELPIQICCSLG"
     gene            complement(523..1917)
                     /locus_tag="VFR82_15545"
     CDS             complement(523..1917)
                     /locus_tag="VFR82_15545"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4503064.1"
                     /translation="MLRLLTTIFLISTGIFVYSYFRELNPGTITVHTGPSTQFELSAV
                     TLVVFSMAVGAVLVALAVGMRQTVHVIGNWRSTRLQRRKEKVDVLHREGTHAFMSKRT
                     SDAVGLFEKALSIDPNRIDSLLWLGNIYRSETNYAEAIRLHQNAHRIDDRNIEILLEL
                     SKDLEGAKRYEEALQALQKILRIEPDNLTGLTRKRDLLIRLEKWSDALEIQHRMAKAN
                     LPEAERRAEGQLLTGCMYEVGRQLLERGHADKARRYFRGAIKKDRTFLPAYIGLGEVL
                     IREGKTKEAVEILKKVYAKTHNVIILHRLEELFLEQGEPSEIIRIYQEALQQDPHNAA
                     LQFYLGKLYYRLEMVDEAFDILSTVEGPQDQLADYHKVMANLFLRKQHMEQAVIELKK
                     ALQFKKRVVVPYVCTACQQDTTEWSGRCRRCGNWNTLLALPWEGGAGAGRDGAPAAGR
                     TVPYQGIASPFETV"
     gene            complement(1922..2395)
                     /gene="rsfS"
                     /locus_tag="VFR82_15550"
     CDS             complement(1922..2395)
                     /gene="rsfS"
                     /locus_tag="VFR82_15550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU4503065.1"
                     /translation="MPWIPIYFNIISTHKRTPIARTSKATALAIARAMLDKKATDVLI
                     LHVAKLTSVADYLVLGSAESDRQSRSIADHVDGVLSQAGAGALSIEGKASSQWVLMDF
                     GDVIVHVFRQDARQHYGLERLWADAKRLAIPGYESPPLLTSKPVTRTKARAAKRA"
     gene            complement(2352..3065)
                     /gene="nadD"
                     /locus_tag="VFR82_15555"
     CDS             complement(2352..3065)
                     /gene="nadD"
                     /locus_tag="VFR82_15555"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU4503066.1"
                     /translation="MQSAGTENSAPDVQTSEPVGVGLLGGTFNPIHNGHLEIARQTRD
                     ALHLKRIVFIPTGDPPHKPQENLAPARDRYEMVRLAVASDPSFTVSDIEVRRPGKSYS
                     IDTIRLLLEQYGGSVPLYFLIGLDAFLELPTWRDPAIALTLCSFVVISRPGAFFQALG
                     SLPLIPRIPRQSLVDLDEGSSSRLEVPIGEQALICLRLPPCEVSASDIRTRIKQGRPT
                     ANLLPPAVDSYILQHHIYS"
     gene            complement(3083..>3411)
                     /locus_tag="VFR82_15560"
     CDS             complement(3083..>3411)
                     /locus_tag="VFR82_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HEU4503067.1"
                     /translation="RLTSRKHWIGFTLRARGTVSLDQGAVEALTKRGKSLLASGIVQI
                     TGSFDAGDPVSCLDPDGKEFAKGLVNCSSDTLSKIKGLKTAEIQQRLGPQEYEEVIHR
                     DNLVIF"
BASE COUNT          694 a          972 c          997 g          748 t
ORIGIN      
        1 ccgagcgagc agcagatttg gatgggcagc tcctgtttga tctctcggac ggcgccggcg
       61 atctcatcga tctcgcgatc caacgggctg cgcccgctga tgacaataca gtaccgctgc
      121 gccttcgacg cggcggcctg ccgcgcaccc gcaaccatct gtgcacgcgg cactaaattg
      181 tatcgttcga tcgccgccgt tgacaccgcc gattgcgagc agtagtggca atcctcctgg
      241 cacgcgccac tcttcgcatt ttgcagcatt tgcaggcgca ccgtcttacc gaaatagcgg
      301 gagcgcacca ggtaagcggc ttgcagcacc tcgagcaggt cgtcgtcagg agcattgagc
      361 agggcacgac actcctgacg cgaaaggatc tcgtcgttga gcgccttctg agcaaacact
      421 gcatagtccg tcatctcagc ccctttcacg gcgatcatcc tcggcgtgga acgcgaagac
      481 gcattcacaa tcgattacgt cctgcgttgt cgagcgaaaa aactacactg tttcgaatgg
      541 agaagcaatg ccttggtagg gtacggtgcg tcccgccgcg ggcgccccat cacggccggc
      601 tcccgcgccg ccttcccatg gaagcgcgag gagggtgttc cagttgccgc atcgacggca
      661 ccgtcccgac cactcagtag tgtcctgctg gcatgccgta cagacgtagg gaacgaccac
      721 gcgtttttta aactgcagcg cctttttcag ttctatgacc gcctgttcca tgtgctgctt
      781 ccggagaaac agattggcca tgaccttgtg gtaatccgcc agttgatcct gggggccttc
      841 tacggtcgac aggatatcga aggcctcgtc gaccatttcc agccggtagt agagctttcc
      901 gagatagaac tggagtgcgg cgttatgagg gtcctgctgc agtgcctcct gataaatgcg
      961 gatgatctcg ctgggttcac cctgttccag aaataactct tccaggcggt gcaaaatgat
     1021 gacgttgtgg gtcttcgcgt agaccttttt caggatttcg accgcctctt tcgtcttgcc
     1081 ttcacggatc agcacctcgc ccaagccgat gtaagctgga aggaaagtgc gatccttctt
     1141 gattgcgccg cggaagtatc gcctggcctt atcagcatgg ccgcgttcca gcaactggcg
     1201 gcccacttca tacatacacc cggtcagcag ctgtccttcc gcccgccgtt ccgcttcagg
     1261 gaggttggcc ttggccatac ggtgttgaat ttcgagcgcg tcgctccatt tctccaaacg
     1321 gataaggaga tcccgcttcc gcgtgagtcc cgtcaggtta tccggttcga ttctgaggat
     1381 cttttgcagc gcctggagcg cctcctcata tcgttttgcc ccttccaggt ctttcgataa
     1441 ctcgagaaga atctcgatat tccggtcgtc gatgcggtgt gcattctgat gcaggcgaat
     1501 ggcctcggca taattggttt cagagcggta aatgtttccc agccacagta atgagtcgat
     1561 ccgattcggg tcgatcgaaa gcgctttttc aaagagtccc acggcgtcgc tggtgcgttt
     1621 ggacataaag gcatgagtcc cttcacgatg caagacgtcg accttctcct tgcgccgttg
     1681 gagccgagta ctgcgccagt tgccgatcac gtgcacggtc tgccgcatgc cgacggcgag
     1741 ggccaccaac accgcgccta ccgccatcga aaagaccacc aatgtcacag cgcttaattc
     1801 aaattgagtc gacgggccgg tgtggacggt gatcgtgcca ggattgagct cgcggaaata
     1861 gctgtaaaca aagatgccgg tactgatcag aaaaatcgtg gtaagcagcc tgagcatggc
     1921 actatgctcg ctttgcggca cgcgcttttg tgcgcgttac cggtttcgat gtgagcagcg
     1981 gcggagactc gtagccagga atggctagac gtttggcatc cgcccacaag cgttccagtc
     2041 cgtagtgttg gcgggcatcc tgtcgaaaga catggacgat cacgtcgccg aaatccatca
     2101 acacccactg agacgaagct ttcccctcga tactcagcgc accagctccc gcttgagaca
     2161 gaacgccgtc gacatgatcg gcaatcgatc ggctttgccg gtcggactct gcggaaccta
     2221 acaccaggta atcggcgacg gaggtcagtt tcgcgacgtg gaggatgagg acatccgtgg
     2281 cctttttgtc cagcatggcc ctcgcaatgg caagggcagt ggccttcgat gttcgtgcga
     2341 tcggcgtcct cttatgagta gatatgatgt tgaagtatat aggaatccac ggcaggcggc
     2401 aacaggtttg ccgtggggcg tccctgcttg attctggtgc ggatgtcgga ggccgacacc
     2461 tcgcacggcg gcaatcgaag gcagataagt gcttgctcgc cgatggggac ctcgagtctt
     2521 gaactgcttc cctcatccag gtcaaccagt gattgcctgg ggatgcgggg aatgaggggc
     2581 aaggatccga gcgcctgaaa gaaggcaccg ggtcttgaaa tgacgacgaa cgaacagagc
     2641 gtgagggcga ttgccgggtc acgccaggtc ggcaactcga gaaacgcgtc caatccgatc
     2701 aagaaataca gggggacgct ccccccatac tgctctagga gaagccggat ggtatcgatc
     2761 gaataagatt ttccaggtcg gcgcacctcg atatcggaga cagtgaagga aggatcagaa
     2821 gcgacggcga gtcggaccat ctcataacgg tctctggccg gcgcgagatt ctcctgcggt
     2881 ttgtgcggcg ggtcgccggt tgggatgaaa acgatccgtt tgaggtgaag ggcgtcccgt
     2941 gtctgccgtg caatctcgag atggccgttg tgaataggat taaaagtgcc ccccaggagc
     3001 ccgacgccga ccggttctga agtctgtaca tcgggtgctg aattttctgt gccggcggac
     3061 tgcatcaccc aacactcacg gctcagaaaa tgacgagatt gtcgcgatgg atgacttcct
     3121 cgtattcctg agggccaagt cgttgctgga tctcggccgt tttcaggcct ttgattttgg
     3181 acagggtgtc ggaagaacag ttcacgagac ctttggcgaa ttctttgccg tccgggtcca
     3241 gacagctgac tggatcgccg gcgtcgaacg aaccggtgat ttggacgatg ccagaggcca
     3301 gcagactctt ccctcgcttc gtcaaggctt cgaccgctcc ctgatcgagg ctgaccgtcc
     3361 ctcgtgcccg cagcgtgaag ccgatccagt gcttgcggct ggtcagtcgg c
//
LOCUS       DASTQY010000487         5525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1732237, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000487 DASTQY010000000
VERSION     DASTQY010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732237"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            465..1463
                     /locus_tag="VFR82_15565"
     CDS             465..1463
                     /locus_tag="VFR82_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4503068.1"
                     /translation="MIDVHHITKRYGQHTAIDRVTFSVAKGEVLAFLGPNGAGKTTTM
                     RILTCFMPATEGRATVAGFDCADQPLEVKRRIGYLPETPPVYQELTVDEYLRFVGRLR
                     GLSGPMLTSAMAREVERLGLSAVQHRLIGNLSRGYRQRVGLAQALLHDPPVLILDEPT
                     VGLDPKQIIEIRELIKSLAGSHSVILSTHILPEATAVCQRVVIINGGRIVAEDTPDRL
                     SARLRHSEKIALTLSRAPGEVEARLRQVTGVQHVHANGPPGHFLLESELGHDIREDVA
                     RLAVTNNWGLLELKSISMTLEDVFLKLTRHEEGLTQPADVASDRGVVNVDKAESSS"
     gene            1460..2227
                     /locus_tag="VFR82_15570"
     CDS             1460..2227
                     /locus_tag="VFR82_15570"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU4503069.1"
                     /translation="MTPVNTILSKELRSHFVSPVVYVVGSVFLLIVGLLAYLYIVFTG
                     AQAIQLMQVQGQAQINLNDLVFRNLFSSIRFVLLIILPILTMRLFAEEKKLRTFELLM
                     TSPIGLNEIVAGKFMSVFLLFLGLLGLTGLIPLTLSLFSDFDWYPVLTGYLGLALLGA
                     LFLSVGVLASALTENQVVAAFVSFGLLLLLWLLAGLGSLLGDSTAGQVVSYLSFMEHY
                     DHLVRGLIDTQDLVYFATALILMLFLTHRVIDSTRWK"
     gene            2224..3747
                     /locus_tag="VFR82_15575"
     CDS             2224..3747
                     /locus_tag="VFR82_15575"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEU4503070.1"
                     /translation="MNLRTIPLGVIGSVLALGGVAAYLFSPEQLWLVTGAESLALLCL
                     ILFLYVHRETFKTFSSRRSTRMGANSLLMVVLFLGILGIVNFLAARHSIRWDFSENQN
                     FSLAPQTHRVIRSLPRDVKITVFTREKDPGYQSYKERLDSYRQASLKIAVEFVDPERQ
                     PKMAQSYGITRSDTAVFESGLQTVRVTNPSEAELTGALIRVSKDDKKRIMFLEGHGEP
                     GTENRDRTGFSLAKETLVKQGYEIGTLSLLTQTAVPDNTAILAIVGPRKPLTNDELNR
                     IHAYVEKGGHLLLMVDPDTQADFTALLSQWGVGLGRGVLVDLQDRLAQGDLTALLVRT
                     FTEHEITQDINAAVLFPLARHITFNDQVGKDWDFVPLARTSPNSWAETDLKGRVVSLN
                     EKEDIKGPLPMAAALTPKTAPAEGKTKSAIVVIGNSTFANNAFVNFPGNSDFFLHTVA
                     WLAQDRDMISIGPREQVFRPFVPNPLQERTLLYVQVIFLPALVLIAGVQVWRKRRRL"
     gene            3723..5111
                     /locus_tag="VFR82_15580"
     CDS             3723..5111
                     /locus_tag="VFR82_15580"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEU4503071.1"
                     /translation="MAQTPPPLMKRYWPTAVMALVFSALGLYLYLVEFPAKESQEREA
                     TTKKKLLTVDEQAITSLTFKTDREELIFERTPDKGWLLIKPIRTETDTREISGLIRAI
                     ALGSVHRVVETNPTSLTPFGLDTPVTTLEIQAGSRSDTLLIGDTGPLTSTLYVFRNSD
                     HAVLLTDLAPKDFVNKSLMTFRRKDLLHRGNVDRIRLTYPTTEIVLYRVSDKPKPKWK
                     IRYPVEAEADINEVRMLLFRLEDLKAMGIVDPGPARDALARTLKQPKVKITVHGEDGD
                     QTVKLYQPNPSSGEAIAETTPDAPLFRVNPTFVKEFTKDLYALQDKRLLGAEEHDIAL
                     LSVKTRSERYTLINQNGEWVLEDRPAEKLNQQVVNLFVSRVANVPAEERVLKQAGPLA
                     PYGLVNPAAEFVATARDGKVTGKLLVGDQANGLAFATGHRLPGIYQIRSDLLKQIPPT
                     AELVAGPGGSSN"
     gene            complement(5112..>5525)
                     /locus_tag="VFR82_15585"
     CDS             complement(5112..>5525)
                     /locus_tag="VFR82_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503072.1"
                     /translation="DNPEKVIEETCAREWSHNSRMRAACIEQQNSVLEKSRSTPVDPR
                     LQHEDLSLIQERCEKNWPDDVRMRLQCQQQEMRAFQKLQGPPPKGISLQDYSVAVAQC
                     SKDFPDDYRLRARCMDEQIAEKRRHQEPIDRSTTQ"
BASE COUNT         1222 a         1552 c         1504 g         1247 t
ORIGIN      
        1 agcaaaaccc tcttattccc cctgataatc ttggtctgag cggtcaccgc tgctccaacg
       61 gtcatgtggt ttttgggacc taaaggggtt ttcaccacga taaggggcgg agtttgcgtt
      121 aaggtgatcg caataaccgt cccgtgcacc ttgtaagtgc tatgtgacct cgcctcccca
      181 acgacggctg agaggcaaga gaccaaacaa atcaggccaa cggcgaacca gtaatacctg
      241 ctcatgacgt caatggaatg ggaagcggtc ctgggacggg cgggactcta acaggacccg
      301 gttgcttctg taaaggttgt cggtgttggc ggcagactca tgttgactgc ctcgagtctc
      361 ctgagtaaga tgcccagcag tgaattttag ctatggtgat cggacgcccg cctcagtaat
      421 ccgtaatccg gaccccatcc accaccgacc ctagattcgt tggcatgatc gacgttcacc
      481 atatcactaa gcgttacggc cagcacacgg ccatcgatcg cgtcacattc tctgtcgcca
      541 aaggcgaggt gctcgccttt ttaggcccaa acggcgcagg gaaaacgacc acgatgcgga
      601 tcctgacctg tttcatgccg gcgaccgaag gcagagcaac ggtggcaggt ttcgattgcg
      661 ccgatcagcc gcttgaggtc aagcgccgga tcgggtacct gcccgaaaca ccgcccgtct
      721 atcaggagtt gacagtcgat gaatatctgc ggttcgtcgg acggctgaga ggactatccg
      781 gtccgatgct gaccagcgcc atggcgcgag aagtggagcg gttgggcttg agcgccgtgc
      841 aacatcggtt aatcggaaac ctctcgcgtg ggtaccggca gcgggtaggc ctggcacaag
      901 cgctccttca tgatccgccc gttttgattt tggacgagcc gaccgtcgga ttggacccga
      961 agcagattat agaaatccga gagctcatca agagcctggc cggatcccac tctgtcattt
     1021 tgagcacgca tattctccct gaggcgaccg ccgtatgtca gcgagtcgtc attattaacg
     1081 gaggccggat cgttgccgaa gatactccgg atcggctgtc tgctcgactt cgccattcag
     1141 agaagatcgc gctgacgctg tcccgagccc ctggtgaagt cgaggcccgc ttgagacaag
     1201 tcactggcgt tcagcacgtg catgcaaatg gtccaccagg acattttctt ttggaaagcg
     1261 agttggggca cgacatcaga gaggatgtcg ctcgtctcgc cgtcaccaac aactggggac
     1321 tcctcgagct caaatcaatt tcgatgacgc tcgaagacgt cttcctcaag ctcacaaggc
     1381 acgaggaagg attgacccag cccgcggatg tggcgtcgga ccgtggggtg gtcaacgtgg
     1441 acaaggcgga gtcatcctca tgacgcctgt gaacacgatc ctgtcgaagg agttacgctc
     1501 gcactttgtg tctccggtgg tgtatgtcgt gggatcggtg tttctgctga tcgtcggact
     1561 gctcgcatac ctgtatatcg tcttcaccgg tgctcaagcc atccaactga tgcaggtgca
     1621 gggacaggca cagatcaacc taaacgatct ggtctttcga aacctgttta gcagcattcg
     1681 attcgtcttg ttgattatcc ttcccattct gaccatgcga ctcttcgcgg aagagaagaa
     1741 actccggacg ttcgaactgc tcatgacctc tccaatcggt ctgaatgaaa tcgtcgccgg
     1801 caagttcatg agtgtgtttc tgctgttttt ggggttactc ggcctgaccg gactcatccc
     1861 cttaaccttg tcgttattca gcgatttcga ttggtatccg gttctgaccg gctatctcgg
     1921 tctcgcgctg ctgggggcgc tatttctttc tgtcggcgtg ctcgcttcag ctttgactga
     1981 gaaccaagtc gtggcagcgt tcgtaagctt cggcctcttg ctcttgcttt ggctgttggc
     2041 aggacttgga tctctccttg gtgacagcac ggccggccag gtcgtgtcat acctgtcgtt
     2101 tatggaacat tacgaccact tggtccgcgg actcattgac acgcaggacc tggtgtattt
     2161 tgccaccgca ctgatcctca tgctgtttct tacgcatcgt gtcatagact ccaccaggtg
     2221 gaaatgaacc tcaggaccat ccctcttgga gtgatcggat cggtgctcgc gcttgggggc
     2281 gtagcggcct accttttctc gccggaacaa ctgtggctcg tgacgggtgc agaaagtctc
     2341 gcacttctct gcttaatcct ctttttgtac gtacaccggg agaccttcaa gactttctcg
     2401 tcacgccgct caaccagaat gggggccaac agcctactga tggtggttct gttcctcggc
     2461 atcctgggga tcgtgaattt ccttgccgcg cgtcactcga ttcggtggga cttttcagaa
     2521 aatcaaaact tttcacttgc gccgcagacg caccgggtga tccgaagcct gccgcgggac
     2581 gtcaagataa cggtctttac gcgtgaaaaa gaccctggct accagtcata taaggagcgt
     2641 ctggacagct atcgccaggc gagtttaaag attgcggttg agttcgtaga cccggaacgc
     2701 cagcccaaaa tggcacagag ctatggcatc acgagaagcg ataccgccgt cttcgaaagc
     2761 ggcctgcaga ccgttcgcgt gacgaatcct tcggaagccg agctcaccgg tgcgctgatc
     2821 cgtgtgtcga aagacgacaa gaaacggatc atgtttttgg agggccacgg ggaacccggc
     2881 accgaaaacc gcgatcgcac cggattttcg ctggccaagg aaaccctggt caaacaaggg
     2941 tacgaaatcg ggactctgag cctgctgact caaactgcgg taccagataa tacagccatc
     3001 ctggcgattg tcggtccgcg taaacccctg accaacgatg aattgaatcg cattcacgcc
     3061 tatgtcgaaa aagggggaca tctgctgttg atggtcgatc ccgacacaca ggcggatttt
     3121 actgctctac taagccagtg gggtgtcgga ttgggccgtg gcgtgctcgt tgatttgcag
     3181 gaccggttgg cccaaggaga cttaaccgcc ctgctggtga gaaccttcac cgagcatgag
     3241 attacgcaag acatcaatgc cgccgtgctg tttcctcttg cccgtcatat cacctttaat
     3301 gatcaggttg ggaaagactg ggattttgtg ccgctcgccc gcacgtcccc aaatagctgg
     3361 gcggaaacag atttgaaggg ccgcgttgta agcctgaatg agaaagaaga tatcaagggg
     3421 ccgctaccca tggccgctgc tctcactcca aaaacagctc cggcggaagg caaaactaag
     3481 tctgccatcg tggtcatcgg gaactcgacc ttcgcaaata acgccttcgt gaacttcccc
     3541 ggcaacagcg acttcttcct ccataccgtc gcctggttgg ctcaagaccg ggacatgatc
     3601 agcatcggtc ccagagagca ggtgttccgg ccatttgttc caaatccact tcaagagcgc
     3661 acgctcctct atgtacaggt cattttcctg cccgccctgg tgctcatcgc aggagttcag
     3721 gtttggcgca aacgccgccg cctttaatga agcgctattg gcctacagcc gtcatggctc
     3781 tcgtattttc cgcgcttggt ctctatctct acctcgtcga gtttccagcc aaagagtctc
     3841 aggaacgaga agcaaccacg aagaaaaagc tgctgacggt cgatgagcag gctattactt
     3901 cgttgacttt caagaccgac cgcgaggaac tgatctttga acggactccg gataaagggt
     3961 ggttgctgat taaacccatc cggacagaga cggacactcg tgaaatcagc ggtctgattc
     4021 gtgcgatcgc gctgggatcg gttcacaggg tggtggaaac caatccgacc tcattgactc
     4081 cgttcgggct cgacaccccg gtcaccactc tcgaaatcca ggcagggagc agatcggaca
     4141 ccttgctgat cggtgacacg ggtccgctca cctctacctt atatgtcttt cgcaattccg
     4201 accatgcggt gctcctgacg gatctggccc caaaagactt tgtgaataaa tccctgatga
     4261 ccttccgaag gaaagacctc ctccaccgag gaaacgtcga tcgaatccgc ctgacctatc
     4321 ccacaaccga aatagtgctg tatcgagtca gcgacaaacc caaacccaaa tggaagattc
     4381 gctatcctgt tgaagcggaa gccgatatca atgaagtgag aatgctgctc tttcggctgg
     4441 aagatctgaa agccatgggc atcgtagacc caggcccggc tcgcgacgcg ctggctcgga
     4501 cactgaagca gcccaaagtc aaaatcaccg ttcacggaga ggatggcgac caaacggtta
     4561 agctgtacca gccgaatccg tcaagcggcg aagctatcgc agaaaccaca ccggatgcgc
     4621 cgctttttcg ggtgaacccg accttcgtca aagaattcac caaggacctc tatgccttgc
     4681 aagacaagcg ccttctcggc gcagaggaac acgacattgc cctccttagc gtgaagacac
     4741 gctcggagcg gtacacgctg attaatcaga atggggaatg ggtcctggaa gatcgtccgg
     4801 ctgaaaaact caatcagcag gtcgtcaatc tgttcgtcag ccgtgtggcc aacgttcccg
     4861 cagaggaacg ggttctcaaa caagccggtc ccctggcgcc ttatggactg gtgaacccgg
     4921 cggcggaatt tgtcgcaacg gcaagggatg gaaaagtgac aggcaaactg ttggtcggcg
     4981 accaagccaa cggtctggcg tttgcgactg gccaccggct cccagggatc tatcagatcc
     5041 gctcggatct cctcaagcaa atccccccca cggctgaatt ggttgcggga cccgggggat
     5101 catcaaattg atcattgggt cgtgctccgg tcgatcggtt cctgatgcct gcgtttctcc
     5161 gcgatctgct cgtccatgca ccgcgctcgc aaacgataat catcaggaaa gtccttcgag
     5221 cattgcgcga ccgcaaccga gtagtcttgc aggctgatgc ccttcggtgg aggaccttgc
     5281 aatttctgaa atgctcgcat ttcctgctgt tggcactgta gtcgcattcg tacatcgtca
     5341 ggccaattct tttcacagcg ttcctggatg agcgacagat cttcatgttg aaggcgggga
     5401 tcaacaggag tcgagcggga tttctcgagg acgctattct gttgctcgat acaggcggcc
     5461 cgcatacgag aattatgaga ccactcgcgt gcgcaggtct cctcgatcac tttttcagga
     5521 ttatc
//
LOCUS       DASTQY010000488        12367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1127111, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000488 DASTQY010000000
VERSION     DASTQY010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 12367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12367
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127111"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VFR82_15590"
     CDS             complement(<1..521)
                     /locus_tag="VFR82_15590"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEU4503073.1"
                     /translation="MNALALYRFGHWCYRRRIPLLPALIYRLIYVLHHAVIPMSVEIG
                     EGSELAYGGLGTVLHERCKIGKFVTIGHQVSIGGRSRRWGVPIIEDRCVIGAGAILLG
                     PISIGAESVVGANAVVLEDVPPRTVVAGAPATVVRKDIDIMDYTCLQPPEEAEALSFR
                     STDNLLVTVIQDSG"
     gene            complement(518..1786)
                     /gene="pelF"
                     /locus_tag="VFR82_15595"
     CDS             complement(518..1786)
                     /gene="pelF"
                     /locus_tag="VFR82_15595"
                     /inference="COORDINATES: protein motif:HMM:NF038011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HEU4503074.1"
                     /translation="MSRAAEPASVTESTPGILNTGQTHHRPAPVESADRIRVMKFVAI
                     FAIGGTERQVVNIGKGLDKSRFDLRFATLNKVGELLAECEAQSWAIDEYRIRRLYGFA
                     TMKKQIAFGKNLRRARTQILHAYGFYPNVFAIPAARLAGVPVTIGSIRDIGDTYTFWQ
                     HQVQKHCLRLADHIVVNAEAIKRDLLQRGYDAERLSVIPNGIDCERFRLPGNGDAVRR
                     EWNIPPGVPVVGVLARLLRIKGQEVFLRAAALIASNNPHVRFVIVGDNNIDQQYKEEL
                     KRLTGRLGLEDRVMFTGFRMDVPDLLAALSVVVSPSLGLEGLSNSLLESMAAGLPVVA
                     TRVGGTPEIVEDGVSGLLVPPGDPEALAAAISRLLQDRMTAKRLGQSARRQVFSRYSL
                     EQAVASNERLYHGLLLRARQKGRLKERAQS"
     gene            complement(1756..3090)
                     /locus_tag="VFR82_15600"
     CDS             complement(1756..3090)
                     /locus_tag="VFR82_15600"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU4503075.1"
                     /translation="MNHAQTQWWRPESATREWRSEDGQPAGYWEPPNTAVPFWATMVF
                     IAILLFSPQAYFPALESIRPAMIPAVIGIGAYVLDRVGSRTPFFIPRREVWVAGALLA
                     WATVTIPLSIWPGGSANFLVGYFLKTLAMSWLLSHSAATLGRLRQTTWCLSIMAVGLG
                     LFALNNYLTGVLVERERVVGNEGALTKNPNDLALMVNIIVPLTMALFLSNRRPVIRLA
                     LFGILVVEGLTVVSTFSRAGFLTLGAILITYAWKLRRRAERVWVNAILIFALLLIPVL
                     PSSYFDRMSTIIHTEQDATGSAGERWSDMMIAVRSALSSPVIGAGIGMNVLAMNEARG
                     GEWRPVHNVFLELTMDLGVPGLLLFAWLLWTSVTGSADVHRQADQTVEMKELAYLAEG
                     IHISLLAFALAAMFHPVSYHPYFYYLAALALAAQAVLQSMRNTCLEQPNRHP"
     gene            complement(3104..4276)
                     /gene="wecB"
                     /locus_tag="VFR82_15605"
     CDS             complement(3104..4276)
                     /gene="wecB"
                     /locus_tag="VFR82_15605"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEU4503076.1"
                     /translation="MKIVVIAGARPNFVKISPLLRAMRRRPDITPVLVHTGQHYDAQL
                     SDQFFTDLGIEAPAYNLEVGSGSHAVQTAEVMKRLEPVLGTEHPDRLVVVGDVNSTIA
                     AALVAVKLGIPVDHVEAGLRSFDRTMPEEINRILTDAIADQLFVSESSGVDNLLREGI
                     PAERIHLVGNVMIDALEACRPRWEQAPVLQRFGLEPTQAYALVTLHRPSNVDDPETLR
                     RIVDALETVSRELTLLWPVHPRTKQALQGNPRVHWRVTKEGNGTIPHGLLAVEPLGYL
                     DCLAVMSRARLVLTDSGGIQEETTVLGIPCLTLRNNTERPVTLTHGTNRLIGTDPEAI
                     VTMARQALGESCRSSDRPPLWDGLASERIVDKLEAGRPSKKNSDPTALRQTASTFS"
     gene            complement(4273..5298)
                     /locus_tag="VFR82_15610"
     CDS             complement(4273..5298)
                     /locus_tag="VFR82_15610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HEU4503077.1"
                     /translation="MRIKPFVENILVLGQSHLATAITAEMKKRRRYRILDLMEPGEPG
                     RVFKDGVPFVGSLDRGLRTIPKTHRIVVALSERRGRLPIEALLDAKRQGVEIEDAVHL
                     YERLTGKLVIDQLQPSHLIFAPELGRGSLHSVWLRVISAVVATLGLVLGAPVFAAIAL
                     LIKWDSAGPVFFIQERVGQDGKKFSLLKFRTMRPADRRPSEWVRDNGDRITAVGRWLR
                     KYRLDELPQLVNVLRGHMNLIGPRPHPASNYSLFMNRIPFYAFRSMVKPGITGWAQVC
                     YGYANSLEEETEKMCYDLYYIKHCSLGLDLNILIRTVKVILLGQQRVSHPEQPDQSTS
                     DYSKEAA"
     gene            complement(5359..6192)
                     /locus_tag="VFR82_15615"
     CDS             complement(5359..6192)
                     /locus_tag="VFR82_15615"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEU4503078.1"
                     /translation="MKEHTNPVLRTSPLESHPLSKSGRTDQRGEPVPHKIAARVKPDE
                     RLVSLVSPATIEAEQYRTLGLMLEQRRHSGLLQVVAVSSPTMGDGKTVTSINLAAALA
                     QSPQSRVLLVDADFRKPSVQAQLGMRDNNALGFRDAILNPDLALKDIVQRLPGSTLSV
                     LTVGRSQVMPHEVIKSTRFAEVLDEARRDYEWIILDTAPLVLAPDCLMMGRSVDGFVI
                     IVCAHKTSRKEIGEALDILGPSKLLGLVFNHDDGLLDSYGYRTYLSASQAQQEDLHHR
                     T"
     gene            complement(6226..7827)
                     /locus_tag="VFR82_15620"
     CDS             complement(6226..7827)
                     /locus_tag="VFR82_15620"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEU4503079.1"
                     /translation="MQDHDRRSIGVAIAEAWRRRKWLGLVCFSLPAAALLSLAMNLPN
                     LYSSTATVLVEQQHIPEGFVKSSVTGEADARLNLIREKLLNRAGLMALINRFNLYSEL
                     RKEAPEEAVVDRMRKDVVLELKELQRQVYGRDNTFGFKVSYRGRNPETVATVANTLAA
                     QYVEENLSMRTNQATETTTLLKKQLNDIKQRHEEDERKIAAFKQRHMGELPEQVEVNL
                     STLGRLNQELTSNSEHQMRVLERRERLGKQLLDLNAGVPATTVVNPDSMTHHLEKLRT
                     ELTELRTRASTKHPDVIRVQGEIAAIEKALAETAKEAKLDPAQSAALLSTPEDIKHAL
                     RELDHELKNLKADEQRLRAEAASIQRRVERSPQHAQEFQQLERDYKMTKDLYFSVLQR
                     YEDAQLAESMEQAKHVGQMRIVDPAMPSKAPVAPNRLKLILGALTLSLGLAAGCMILA
                     EQRDRSFHDVESLREFTKVPILVRIPEIVTKADRIRAYRRFTFKAAASMIGLVLIVGV
                     FSYIAWDNDQLVWMLSESKPTQQNR"
     gene            complement(7843..8487)
                     /locus_tag="VFR82_15625"
     CDS             complement(7843..8487)
                     /locus_tag="VFR82_15625"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEU4503080.1"
                     /translation="MKISRFVIIIGGVVALLSAAPCFAADTPSSSKDAGVSNNSLSDQ
                     DHALAQAQEVGEYRIGPEDVLDISVWNNSAISRTVPVRPDGKISLPLVNDVQAAGLTP
                     NQLRSVLLKKLTEYVPSPEVSVIVREVHSFKVSVIGEVKKTGRHELKGRATVLDILAM
                     AEGFGEFAGRGRIVVLRPEGKTMRQIPFNYNKVMSNNGALENFFLQPGDIIVVP"
     gene            complement(8514..9815)
                     /locus_tag="VFR82_15630"
     CDS             complement(8514..9815)
                     /locus_tag="VFR82_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503081.1"
                     /translation="MTTGIRWGFARGRKGDAMRVLRWACCVMTLTMLVDPATVKAQLL
                     QGIPRSPIDTMSGSTERMPEGVYLMPWLATGVVYDDNVFFSTRDRRQEDVFLRVTPGL
                     QGSYQSTRLTVIANYRFDSEVYNKFSELSTVQQRQFGTVEMRGRPSNNLNLNGIVGYA
                     QTRTPYELNFLTSAQTARFETERFFINPSAEYRIDSLTKFRGEYAFSKDIFADEVSIN
                     SHIFNLGLERKVSAHDWIGPAYVGRHFTFGGNLESPIAGFIGGNAEDVTSHAFLVSWA
                     HDFTTDTRLDVRAGPRFTNGSLDDRPEAYVGLRRRIPNGELTLGYTSALTTIIGTVGA
                     TRTDSVGVRVVYEPVKHFTLTAAPSAAWVKSSSFQGTIYTAYVEAAYQFNKFVTAKGS
                     AYFSYQESDFFTDGTVTAENVVIPRNVYWLRLEFTYPSRWQ"
     gene            complement(9812..10405)
                     /locus_tag="VFR82_15635"
     CDS             complement(9812..10405)
                     /locus_tag="VFR82_15635"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HEU4503082.1"
                     /translation="MGNHGQTEGGGAFSLLATGDENGISETIETGPRRWYALWTHSHC
                     EQLVRGQLDGRGFEVFLPTIGVWSLRAGVKHRIRRPLFPGYLFVRDKLSGARYVELLK
                     ARGLVGVLGGQDGGPGEIPDTEIDAIRALVNSELPASPYPYLQTGQRVRITEGPLTGV
                     EGVLVDRRAGQSLLVLSVHLFRRSIAVEVDCALAVPA"
     gene            10780..11898
                     /locus_tag="VFR82_15640"
     CDS             10780..11898
                     /locus_tag="VFR82_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4503083.1"
                     /translation="MKIAQLSPLWESVPPQLYGGTERIVSYLTEELVRQGHEVTLFAS
                     GDSKTAAHLRACCDRALRLHDNVMINRDAPNILMQEWALGAESGHYDVVHSHLDFLSF
                     PMSRRAHAPVVTTLHGRLDLPELQPIFKEYSELPVVSISNAQRKPLPGANWAGTVYHG
                     IPKDLYSLHDRPGSYLAFLGRISPEKCPDHAIEVAKRVGMPLRIAAKVDAADHAYFKQ
                     KIERLLDHPLVEYVGEINDAEKNDFLGDAAALLCPYDWPEPFGLVLIEAMACGTPVLA
                     YRQGSIPEVIDHGVTGYICGNIGEMAQAVAGLPLIDRKRCREVFEKRFTVERMAKDYL
                     NLYESMVAAPHRPRLLTRPASHARVGAIHTLAPQADVA"
     gene            complement(11955..>12367)
                     /locus_tag="VFR82_15645"
     CDS             complement(11955..>12367)
                     /locus_tag="VFR82_15645"
                     /inference="COORDINATES: protein motif:HMM:NF014818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HEU4503084.1"
                     /translation="STSGAATATARALPSLRGRFDATAVRAPVPICSIADVVMVTART
                     TSVEEVNTLFLEEARSERYEGVVGVTEDPVVSTDIIQDPRASIIDREMTQVVDGDLVK
                     VMSWYDNEWGYASQMVRQAVSMAQIVPVEPALSR"
BASE COUNT         2579 a         3673 c         3482 g         2633 t
ORIGIN      
        1 ccggaatctt gaatgaccgt gacgagcaga ttatcggtcg atcgaaacga cagagcttca
       61 gcttcctcgg gaggctgcaa gcacgtgtaa tccatgatgt cgatgtcctt acggacgacg
      121 gtggcaggag ccccggccac caccgtcctg ggcggcacgt cctccaacac gacggcgtta
      181 gctccgacga ccgattctgc gccgatcgaa atcggcccca gtaagatggc tcccgcaccg
      241 atcacgcaac ggtcttcgat gatcggcacg ccccatcggc gcgagcgccc gccgatcgac
      301 acctgatggc caattgtcac gaacttgccg atcttacagc gttcatgcaa gaccgttccg
      361 agtccgccgt acgccaactc cgatccctcg ccgatctcaa ccgacatcgg aatgacggcg
      421 tgatgaagta catagatcaa ccgatagatc agggccggca ggaggggtat ccgccgccgg
      481 tagcaccagt gtccgaagcg atataacgcc aacgcattca tgactgtgcc cgttctttta
      541 gccgcccctt ttgtcgtgcc cgaagcagca ggccgtgata cagccgttcg ttggaggcaa
      601 cggcttgttc gagcgaatac cggctaaata cctgacgtct ggcggattgt ccgaggcgct
      661 tcgccgtcat cctgtcttgc agcagcctgg agattgccgc ggccaaggcc tctggatctc
      721 ccggtggaac caacaggccg ctcaccccat cttcgacgat ctccggagtg ccccccaccc
      781 gtgtcgccac cactggaaga cccgctgcca tggactccag gagcgaattg gacaatcctt
      841 ccaagccgag cgacggtgac acgacgacgg ataacgccgc cagaagatct gggacatcca
      901 tgcgaaagcc tgtgaacatg acgcgatctt ccaggccaag ccggccggtc aaccgtttaa
      961 gctcttcttt gtactgctgg tcgatgttat tgtctcccac gatcacaaac cggacatgcg
     1021 ggttgttcga tgcgatcaac gcggcggccc ggagaaatac ttcctgtccc ttgatccgca
     1081 gcagcctggc caaaacgccg acaacgggca cgccgggagg aatgttccat tcccgacgca
     1141 ccgcatcgcc gttgcccggc agacggaagc gctcgcagtc gatgccgttc ggtatcacgc
     1201 taaggcgctc ggcatcgtat ccgcgctgta acaaatctcg cttgattgcc tccgcgttca
     1261 ccacaatatg gtcggccagg cggagacagt gtttttgcac ttgatgctgc cagaaggtgt
     1321 aggtgtcgcc gatgtcgcgg attgacccga tcgtgacggg tacgcctgca agtcgtgcgg
     1381 ccggaatggc aaacacgttc gggtagaagc cgtaggcatg caggatctgc gtccttgccc
     1441 gacgcaagtt ttttccaaac gcgatctgtt tcttcatcgt ggcaaaacca tagagtcgac
     1501 gtattctgta ttcgtcaatt gcccaggatt gcgcctcgca ttcagcgaga agttcaccga
     1561 ccttattcaa ggtggcaaaa cgtaaatcga agcgtgactt gtccaaaccc tttccgatgt
     1621 tcaccacctg gcgttcggta ccgccaatgg caaaaatggc gacgaacttc ataacccgta
     1681 tgcgatctgc ggattcgaca ggcgccggcc gatggtgagt ttggcccgtg ttgaggatgc
     1741 ccggcgtgga ctcggtcacg gatgccggtt cggctgctcg agacatgtat tcctcattga
     1801 ctgcaagacg gcctgtgcag cgagagcgag ggccgccaga taataaaagt agggatggta
     1861 actcactgga tggaacatgg ccgccagggc aaaggcaagc aggctgatat ggatcccctc
     1921 ggcaagatag gcaagctctt tcatctcaac tgtctggtcg gcctgtcggt ggacatcggc
     1981 tgagccagtc acacttgtcc acagtaacca cgcaaacaac aggaggcccg gaaccccgag
     2041 gtccatcgtc agctcgagga aaacattgtg gacgggtcgc cattcccctc cccgcgcctc
     2101 attcatggcc agcacgttca tgccgattcc tgcgcctatt accggcgacg acaaggcgga
     2161 acggacggcg atcatcatgt ccgaccaccg ctcgccggca gagccggtcg catcctgttc
     2221 cgtgtggatg atggtgctca tgcggtcgaa gtagctcgat ggcagcaccg ggatgagaag
     2281 cagtgcgaaa atcaggatgg cattgaccca tacccgctcg gcccggcgtc gaagcttcca
     2341 cgcatacgtg atgaggatag ctcccagcgt gagaaatcct gctcgcgaga aggttgagac
     2401 gacggtcaac ccttcgacga cgagtatgcc gaacaatgcc agacgaatca ccggccgacg
     2461 gttgctcaga aataacgcca tggtgagcgg gacgatgatg ttcaccatca acgcgaggtc
     2521 gttgggattc ttggtcaacg ccccctcgtt gccgaccaca cgctcacgct cgaccaagac
     2581 ccccgtgagg tagttgttga gcgcgaacag tcccagcccg accgccatga tgctcaagca
     2641 ccaggtggtc tgtctgagcc ggccaagtgt ggctgccgaa tgactcagca accaggacat
     2701 ggcgagggtt ttcaaaaagt atccgaccaa aaaattcgca ctgccgcctg gccaaataga
     2761 caaaggaatg gtgacggtcg cccatgccag caacgcgccg gccacccaca cctctcgtcg
     2821 aggaatgaag aacggtgtgc gagaaccgac ccggtccagc acatacgcac cgataccgat
     2881 gacggcgggt atcatcgccg gccggatcga ctccaaggcg gggaaatagg cttgcggaga
     2941 aaagagaagg atggcgatga aaaccatcgt ggcccaaaag ggaacggccg tgttcggggg
     3001 ctcccagtaa ccggccggct ggccatcctc ggaccgccac tcccgggtag ctgattccgg
     3061 cctccaccat tgagtctgag cgtggttcat ggggttgtag ggatcaagag aaggtggagg
     3121 ctgtctggcg gagggcagtg ggatcggaat ttttcttcga cggccgccct gcctcgagct
     3181 tgtcgacgat gcgctcggat gccaagccat cccagagagg aggccgatcg cttgacctgc
     3241 aggattcacc caacgcctgc cgtgccatcg tgacgatggc ctcaggatca gtaccgatca
     3301 gtcggttggt gccatgggtc aacgtcactg gtcgctcggt attgttgcgc aaggtaagac
     3361 aaggaatgcc cagcactgtc gtttcctctt gaataccccc tgaatccgtg aggaccagac
     3421 gagcccggct catcactgca agacaatcga ggtatccgag cggttcgacc gccagcaatc
     3481 catgaggaat cgtgccattg ccctctttgg tgacacgcca atgcacacga gggtttccct
     3541 gcaacgcctg tttcgtccgc ggatggacgg gccacaagag tgtcagctcg cgggagaccg
     3601 tctcaagcgc gtccacaatc cgtctgagcg tctcgggatc gtccacgttc gagggacgat
     3661 gcagggttac cagcgcatat gcttgcgtcg gctcaagccc gaaacgctgc aagacgggcg
     3721 cctgttccca gcgaggccga caggcttcca gtgcatcgat cattacattg cccactaagt
     3781 gaatgcgttc ggctggaatc ccctcccgca gcaagttgtc caccccactg ctttcgctga
     3841 cgaacaactg gtctgcgatc gcgtcggtca agatgcgatt gatttcttcc ggcatcgtac
     3901 gatcgaagct gcgaagcccc gcttcgacat gatccactgg tatgccaagt ttgaccgcca
     3961 cgagagccgc ggcgatcgtg ctgttgacgt cccccacaac cacgaggcgg tccggatgtt
     4021 cggttccgag cacaggctcc agacgcttca tgacttcagc ggtctggacc gcgtgcgatc
     4081 ccgatcccac ctctaaattg taggctggcg cctcgatccc gaggtctgta aaaaactgat
     4141 cggacagttg tgcatcataa tgttgtccgg tatgcacgag cacgggtgtg atatcgggac
     4201 gccgacgcat cgcccgcagc agcgggctga ttttgacaaa atttggtcgt gccccggcaa
     4261 tgacaacgat cttcatgccg cttccttgga atagtcggaa gtgctctggt caggctgttc
     4321 tgggtgggag actctctgtt gacccagaag gatcactttg accgtccgga tcagaatgtt
     4381 gagatcaaga cccaatgagc aatgcttgat gtaatacaag tcgtagcaca tcttctcagt
     4441 ctcctcttcg agactgttgg catatccata gcagacctgt gcccaacccg tgatgcctgg
     4501 cttgaccatg gaacggaatg cgtaaaatgg aatgcgattc atgaagaggc tgtaattgga
     4561 agccggatgc ggtcgagggc cgatgagatt catgtgcccc cgtagcacgt tgacgagttg
     4621 cggcagttca tccagccggt atttgcgtag ccagcgtccg acggcggtaa tacggtcgcc
     4681 gttatcgcgg acccattcgg atggacgccg gtcggcaggg cgcatggtac ggaacttcag
     4741 cagagaaaac ttttttccat cctgcccaac tcgctcctgt atgaagaaca ccggaccggc
     4801 ggaatcccat ttgatcagca gcgcgatggc cgcaaaaacc ggagcgccga ggaccaggcc
     4861 gagcgtagcc accacggcgc tgatcacccg cagccacact gaatggaggg agccgcgccc
     4921 cagttcgggg gcaaaaatca aatgactggg ctgcaactga tcaatcacga gtttgcccgt
     4981 caatcgttca tacaaatgaa ccgcatcctc gatctcgacc ccttggcgct tcgcatcgag
     5041 cagcgcttca attggcaagc gacctcgacg ctcgctcagg gcgacgacga tccggtgtgt
     5101 cttcggaatc gtcctcagcc cccggtcaag tgatccgacg aacggaaccc catctttgaa
     5161 tacgcgccct ggctctcccg gttccatcag atccaggatg cggtatcgcc gccgcttttt
     5221 catttcagcg gtgatcgcag tcgcgaggtg gctctgcccg agaacgagga tattttccac
     5281 gaaaggcttt atgcgcatag tgttgtactc tggttcgggg tggtgatggt gcttgtcggt
     5341 tccaatctgt tcttcagctt acgtacggtg atgcaagtcc tcctgttgag cctgagatgc
     5401 ggagagatag gttctatatc catatgagtc gagcaggccg tcgtcgtggt tgaaaaccaa
     5461 ccccagcagc ttggatgggc ccaggatgtc caatgcttca ccaatttcct tgcgtgatgt
     5521 cttatgcgcg cagacgatga tgacaaatcc atccacactt cgccccatca tcaagcaatc
     5581 tggagccagc acgagcgggg cggtatccag aatgatccat tcgtagtcgc ggcgcgcttc
     5641 gtcaagcacc tcggcgaagc gcgtcgattt gatcacctcg tggggcatga cctgggatcg
     5701 gccgaccgtc agaaccgaca gtgtggaccc cggcagccgc tgcacgatgt ctttgagggc
     5761 aaggtcggga ttcagaattg cgtcacggaa accaagcgca ttgttgtctc gcatcccaag
     5821 ttgtgcttgt actgagggct ttcgaaagtc ggcgtcgacg agcaggacac gggactgagg
     5881 agattgtgcc agggctgccg ccaggttgat cgaggtgacc gtcttcccgt cccccatcgt
     5941 ggggctcgag accgccacca cctggagaag cccggaatgg cggcgttgct cgagcatcag
     6001 tccgagcgtt cgatattgtt ccgcctcgat cgtggcgggg gagacaagac tcaccagccg
     6061 ctcgtccggc ttgacccgtg cagcgatttt gtgcggcacc ggttccccac gctggtcggt
     6121 tcggccggat ttggacaagg ggtgagactc caaaggcgac gtccgcaaaa cgggattcgt
     6181 gtgttctttc atgtcgtcaa tccttcaaca atgagcgatt ggcccttacc ggttttgctg
     6241 tgtgggttta ctttcactga gcatccaaac cagttgatcg ttgtcccagg cgatgtacga
     6301 aaacacgccg acgatcagca ctagaccgat cattgaggcc gccgccttga atgtgaatcg
     6361 ccgataggct ctgatccggt cggctttggt gacgatttcc gggatgcgca cgagaatggg
     6421 gacttttgta aactcacgca agctttccac atcatggaag gaccgatccc gctgttcggc
     6481 cagtatcatg caccccgcag ccaggcccaa cgacaacgtc agggctccaa gaatcagttt
     6541 gagccgattg ggcgccaccg gcgctttcga aggcatggca ggatcgacga tgcgcatctg
     6601 cccgacatgc ttggcctgct ccatcgattc ggccagttga gcgtcttcgt agcgctggag
     6661 cacggaaaaa tagagatcct tcgtcatctt gtaatcgcgc tccagttgct ggaactcctg
     6721 cgcgtgctgc ggagaccgtt cgacccgccg ctgaatcgag gcggcttccg cccgcaaccg
     6781 ctgctcatcc gctttcagat ttttcagttc gtgatcaagt tcgcgcaagg catgtttgat
     6841 gtcctccgga gtggacagca gcgccgcgga ttgagcaggg tccaacttcg cttctttggc
     6901 cgtctcggcc agagccttct cgatcgccgc gatctcgccc tggacccgaa tgacatctgg
     6961 atgcttggtg ctcgctctcg tgcgtagttc agtgagctcc gtgcgaagct tttcgaggtg
     7021 atgcgtcatg gaatcaggat tgacgactgt cgtggccggg acacctgcat tcaaatcaag
     7081 cagctgcttc cccaaccgtt cccttcgctc gagcacacgc atttggtgtt cgctgttcga
     7141 cgtcagctcc tggttcagcc gcccaagcgt tgaaaggttg acttcgacct gctcgggcag
     7201 ttcccccatg tggcgctgct tgaaggccgc gatcttccgc tcgtcctctt catgtcgttg
     7261 tttgatatcg ttgagctgct ttttcagcag ggtcgtggtt tccgtcgcct gattggtgcg
     7321 catgctcaga ttttcttcca cgtattgggc ggccagagta ttggcgacgg tcgcgacggt
     7381 ctccggatta cggccgcgat aactcacttt gaagccgaag gtgttgtccc ggccgtagac
     7441 ctgccgctgc aattctttca actcaagcac gacgtctttg cgcatgcgat cgacgacagc
     7501 ttcttctggg gcctccttcc gcaattccga atagagattg aatcgattga tcagcgccat
     7561 caagccggcg cggttcagca atttttcacg gatcaggttg aggcgcgcgt cggcttctcc
     7621 ggtcacggaa gacttcacaa agccttccgg aatatgctgc tgctcgacca gcacagtggc
     7681 cgttgaactg tagagattgg gcaaattcat cgcgaggctc agcaaggcgg cggcaggtag
     7741 cgagaagcag acaagcccaa gccattttct gcgccgccat gcctcggcta tcgcgacacc
     7801 gatcgatcgt ctatcgtggt cttgcatgat cgctccacac ggttatggga cgacgatgat
     7861 gtcacccggt tgtaggaaga aattttccag cgctccgttg ttggacatga ccttgttgta
     7921 gttgaatgga atttgtcgca tcgtcttgcc ctcaggtcgc aggacgacaa tgcgccctcg
     7981 tccggcgaat tcaccgaacc cttcggccat cgcgagaatg tctagcacgg tcgcgcgtcc
     8041 cttcagttca tgcctgccgg ttttcttcac ctcaccgatg accgagacct taaagctgtg
     8101 cacctctctg acgatgacgg agacttccgg cgagggaaca tactccgtca gctttttcaa
     8161 aagcacgctc cgcagctggt tgggggtgag tccggctgcc tggacatcgt tgaccagcgg
     8221 cagcgatatc ttgccgtccg gtcggacggg aacggtccgg ctaattgcgc tattgttcca
     8281 gacagagatg tccagtacat cctccggtcc gatcctatat tccccgactt cctgcgcctg
     8341 cgctaaggcg tggtcctggt ccgacaggct attattcgat acgcccgcgt cctttgagga
     8401 agacggcgtg tcggctgcaa aacaaggcgc ggcgctcagt agcgccacca caccaccgat
     8461 gatgattaca aatcgtgata tcttcatata aacctccggt ccactatgga cagttactgc
     8521 caccgtgacg gatacgtgaa ctcgagccgc aaccaataca cattgcgagg gatgacgaca
     8581 ttctcggccg taacggtccc gtcggtgaag aagtcgcttt cctggtaact gaaataggcc
     8641 gaccctttgg ccgtcacaaa cttattgaat tgataggcgg cctcgacata ggcggtataa
     8701 atcgtgcctt ggaacgaact gcttttcacc catgcagcac tcggtgccgc ggtcagagtg
     8761 aaatgcttga ccggttcata gacgacacgc actcccacgc tgtccgtacg tgtggctccg
     8821 acggtgccga taattgtggt gagcgcactg gtatagccga gagtcagttc gccgttcggg
     8881 atacgacgcc gcaggcccac atacgcttcc ggtcgatcat ccagcgaccc atttgtgaag
     8941 cgggggcccg cgcggacgtc aagccgcgtg tccgtcgtaa aatcatgggc ccagctcacc
     9001 aggaaagcgt gggacgtgac gtcttcggca ttccctccta tgaaccctgc gatcggactt
     9061 tcaagattcc ctccaaatgt gaagtggcgc ccgacataag ccggcccgat ccaatcgtgc
     9121 gcgctcacct tccgttccaa accgagattg aaaatgtgcg aattaatgct cacctcgtca
     9181 gcaaagatgt ccttggaaaa ggcgtattcg ccacgaaatt tggtcaacga atcgatgcgg
     9241 tactcggcgc tcggattgat aaagaatcgc tctgtctcaa aacgggctgt ctgtgctgac
     9301 gtgagaaaat tcaattcgta cggcgtgcgg gtttgcgcat aacccacgat gccgttgaga
     9361 ttgaggttgt tcgacggacg tccccgcatt tccacggtgc cgaattgccg ttgctgaacc
     9421 gtcgatagct cgctgaactt gttgtacact tctgaatcaa accggtaatt ggcgatgacg
     9481 gtcaggcgcg tcgattgata gctgccctgc agacccggcg tcacacggag aaacacgtct
     9541 tcctgtcgtc ggtcccttgt cgaaaagaac acgttgtcgt cgtagactac gcccgtcgcc
     9601 aaccagggca tcagataaac tccctcaggc attcgttcag tggacccgct catcgtgtcg
     9661 atcggggaac gcggaatccc ttgtagcaat tgggccttca ccgtagctgg atcaacaagc
     9721 atcgtcagcg tcatcacgca acaggcccat cgcagtacac gcatcgcatc gcccttacgg
     9781 cctcgtgcga agccccatcg aatcccagtg gtcatgccgg aacggccaat gcacagtcga
     9841 cctcgacggc gatgcttcgg cggaagagat gcacggacaa caccaagagg ctttgtccgg
     9901 cccgccggtc caccaacaca ccttctacgc cggtgagcgg cccttcggta atgcgcaccc
     9961 gctggccggt ctgaaggtac ggatagggtg atgccggcag ctcggagttc acaagcgcgc
    10021 ggatcgcatc gatctcggta tcgggaatct ctcccggacc tccgtcttgt ccgcccagca
    10081 cgcccaccag tcccctcgcc ttgagcagct cgacgtaacg ggcaccgctc agcttgtctc
    10141 gtacgaagag atacccggga aacaagggcc ggcgaatcct gtgtttgaca cccgcacgca
    10201 atgaccagac accgatagtg ggaagaaaga cttcgaaacc cctgccatcg agctgtcctc
    10261 tcaccaactg ttcgcaatgg ctgtgggtcc acagggcata ccaccgacgc ggcccggtct
    10321 cgatcgtttc ggagattcca ttctcgtcgc cggtcgcgag aagtgaaaag gctccgcccc
    10381 cttcggtttg gccatggttc cccatcccga acctccattg ccttcttccg cagccgacga
    10441 tccttcaatc cccgggaacc gacgtctgcg cctcggtgaa caagctcggc ggtttcctag
    10501 ccccgccggt ttgtatcccc ctgttgtgga tcaccgtaac gaatacatga ggtctggact
    10561 atccgtgatg tcactgaaac tgatggggag aatacctgag ggcgtatagg tagtattgtg
    10621 gaggcttcgt caagggggct actagtcact gccctggggt agcgaacgcg cctctctcta
    10681 ggagtttccc tagttcgagt cgcgcctgct cttcgccata gtaaagcaac tcaccatttg
    10741 ggtttcgctc gaacctctat tttcacagaa aggaccgtca tgaagatcgc tcagttgtct
    10801 ccgctctggg aaagcgtccc accccaactg tacggtggga ccgaacgaat cgtctcctac
    10861 ctgacggagg aactcgttcg tcagggtcac gaggtgacgc tctttgcgag cggggactcg
    10921 aagactgcag cgcatctcag agcctgctgc gatcgtgcac tgcgtctcca cgataatgtg
    10981 atgattaatc gcgacgcgcc caatatcctc atgcaggaat gggccctcgg cgctgagtca
    11041 ggacactacg acgtggtgca ttcgcatttg gatttcctgt cgttcccgat gagccggcgc
    11101 gctcacgcgc cagtggtgac gacgctgcat ggtcgcttgg acttgccgga gttgcagccc
    11161 atcttcaagg agtattcgga actgccggtg gtctctattt caaatgctca gcgaaaaccc
    11221 ttgccaggcg caaactgggc agggaccgtc tatcacggaa tccccaagga cttgtactcg
    11281 ctacatgacc gccctggttc ctatctggca ttccttggtc ggatttcgcc ggagaagtgc
    11341 ccggatcatg cgattgaggt ggcaaagcgt gtcggtatgc ccctccgcat cgccgcaaaa
    11401 gtcgatgcag cggaccacgc ctatttcaag cagaagatcg agcggctgct cgaccatccg
    11461 ttggttgaat acgtcggcga aatcaacgac gccgaaaaaa acgattttct gggtgacgca
    11521 gccgctctcc tttgccccta cgactggcct gaaccgttcg gactcgtgtt gatcgaggcc
    11581 atggcctgcg gcacgccggt attagcctat cgtcaggggt cgattcctga ggtcatcgat
    11641 catggcgtca caggttatat ctgcggcaac atcggtgaaa tggctcaagc ggttgcggga
    11701 cttccactca ttgatcgaaa acgatgtcgc gaagtcttcg aaaagcgatt taccgtcgag
    11761 cgaatggcga aggactatct caatctctat gagtcgatgg tggctgctcc tcatcgaccc
    11821 cggctcctga cgcgacccgc gagccatgcg cgtgtcggtg ccatccacac actggccccc
    11881 caagctgacg tagcgtagct gatgaatgct gagtctcctt cgggagactc agcactcccc
    11941 gagtattcct ttgtttaacg ggagagggcg ggttcgacag gaacgatttg ggccatgctg
    12001 acggcctgcc gcaccatttg gctggcgtaa ccccactcat tgtcgtacca actcatcacc
    12061 ttcacgagat cgccgtcgac gacctgtgtc atctcgcggt cgataataga ggcccgcggg
    12121 tcttggatga tgtcggtaga gaccaccggg tcctccgtga cgcccacgac accttcataa
    12181 cgctcactcc tcgcctcttc gagaaacagc gtgttgactt cctcgaccga agtggtgcgt
    12241 gctgtcacca tgaccacatc cgcaatgctg cagatcggga ccggcgctcg gactgccgtc
    12301 gcgtcgaatc gtcctcgcaa tgagggcaag gcccgtgcag tcgccgtagc cgctccgctg
    12361 gtcgagg
//
LOCUS       DASTQY010000489         2864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1771151, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000489 DASTQY010000000
VERSION     DASTQY010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771151"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..446
                     /locus_tag="VFR82_15650"
     CDS             75..446
                     /locus_tag="VFR82_15650"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEU4503085.1"
                     /translation="MHFESGLLSVEAGGEFSLEDAKQAFLELLEAVAQHRAQRILFDA
                     RNVKGKPRDMERFYYGEFAALKTHTIVVEYNIVPQFAYVLHEPLRDRTRLGETVAVNR
                     GMNVKTFETPEDAIEWLTSAR"
     gene            715..2862
                     /locus_tag="VFR82_15655"
     CDS             715..2862
                     /locus_tag="VFR82_15655"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4503086.1"
                     /translation="MDGTERNRVEERLTEQARLKSFSARIGTHLIECGDFAEMLRRCA
                     QTMVDDLGAAFARIWTVNEQHNVLELQASAGLYTNLEGRHARIPVGSLKIGLIAAQGR
                     PHLTNHVIGDPRIPEQDWARSERMVAFAGYPLVVEERVIGVMAMFARSALSDSTLHAM
                     SLVADHIGLAIQRKLAEQRLRQKQERLTLALQAGRMGVYDWDMVSDAVWWSPETYSLF
                     GVSRDEFVPTPKGFEKIIHPEDRESVWQQFTEALHNGADCAYEFRIIRPDGTLRWIAN
                     LGRTQYSTTGRPIRHYGIASDITERKQWEDVLRDSENRLRAFSGQLEQLVEKRTEELM
                     QSHDRLRELATELNLAEQRERKRLAAELHDHLQQLLVLGRLKLGQGKEFVESLPKCAQ
                     LINEADGILSEALTYTRTLVAELSPPVLRDHGLAAGLTWLGAYMQKHDLAVTVTVPEA
                     TLQLPEDQALLLFQSVRELLMNAWKHAATGKADVTMETDDNRLRVQVHDDGRGFEVAV
                     AETTTAHKLASKFGLFSIRERMKALGGSFEIESAPHQGTTATLTLPLGAASSETEVEP
                     RPFEVSQDGSETPNASPLPSGAQTRTVGVLLVDDHAMVRQGLRMMLEDYPDVEVVGEA
                     CDGEEALVCTEQLHPAVVVMDINMPKMNGIEATAYLKTRYPHIAIIGLSVNAGEENQK
                     AMKRAGATSLITKEAAVDQLYAAIQATSAPTRS"
BASE COUNT          663 a          763 c          864 g          574 t
ORIGIN      
        1 caatatgcga actgcaccgt tagctgattg tacttagcgg ctgtctttcc ttaacaatga
       61 gcctacaaca gaagatgcat tttgagtcgg gccttctcag cgtagaggca ggcggagagt
      121 tctcgctgga agatgccaaa caggccttcc ttgagctgct tgaagctgtt gcccaacata
      181 gggctcagag aatattgttc gatgctcgga acgtgaaggg aaagcccagg gacatggagc
      241 gcttctacta cggcgaattc gcagcactaa aaactcacac gattgtcgtt gagtacaaca
      301 ttgtgccgca gtttgcgtat gtgctccatg agcctcttcg tgaccggaca agattagggg
      361 aaaccgttgc cgtgaatcga gggatgaatg tcaagacgtt tgagactcca gaagacgcta
      421 tcgaatggct aacaagcgcg cgctgattcc tcgcattgtc tattaacaca cagctgatgc
      481 gtttgcttgt ctaattcttt cagtcctacc tcagcgaatc ccccagacta taattcctgt
      541 atagcaattg cgaagagaag gggggagtgg tgacatttgc aaaagttcgt aggacgaagt
      601 agctgtcaaa agtgacagtt tgaagttagg tcgatctttg tcatagtgcg ccgcctcttc
      661 tcatgcagaa gactcggcga tgatggtgcg aaagtaaatt tatggatcag accgatggat
      721 ggcaccgagc gcaaccgagt tgaagaacgg ctcacggagc aagcccgcct gaaatccttc
      781 agcgcgcgga tcggcaccca tctgatcgag tgcggtgact tcgccgagat gctgcggcgg
      841 tgcgcgcaga cgatggtgga cgatctgggg gcagcctttg cccgcatctg gaccgtgaac
      901 gaacaacaca acgtgctcga gctccaggcc agtgccggcc tctacaccaa cctggagggc
      961 cgtcacgctc gcatccccgt gggcagtttg aaaatcggcc tgatcgcggc acagggaaga
     1021 ccgcatctga ccaatcatgt gattggggac ccccgcatac ctgaacagga ctgggcccga
     1081 agcgagagga tggtcgcctt cgccggttat ccactcgtcg tcgaggagcg cgtcatcggc
     1141 gtgatggcga tgttcgcccg ctccgcgcta tcagattcga ccctgcacgc gatgtcgctg
     1201 gtcgcggacc acattgggtt ggcgattcag cgtaagctgg cggaacaacg attgcggcag
     1261 aaacaggaac gcctcacgct ggcgctccag gctggacgga tgggcgtgta tgactgggac
     1321 atggtgtccg atgcggtctg gtggtcgccg gaaacgtata gtctcttcgg cgtctctcga
     1381 gacgagtttg tcccgacgcc caaaggcttt gagaagatca tccatcccga ggaccgcgaa
     1441 agcgtgtggc aacagtttac ggaagcactc cacaacggag cagattgtgc ctacgagttt
     1501 cggattatcc gcccggacgg gaccctccgc tggatcgcga atctggggcg aacccaatac
     1561 agcaccacgg ggagacctat ccgccattat ggcatcgcga gtgacatcac cgagcgcaaa
     1621 cagtgggaag atgtcttgcg tgacagcgag aatcggctgc gggcgttctc tggccaattg
     1681 gagcagttgg tggagaaacg aacggaggaa ctgatgcagt cccacgaccg cttgcgtgag
     1741 ctcgccaccg agctcaacct tgccgagcaa cgggaacgca agcgactggc cgcggagctc
     1801 cacgatcacc tccaacagtt gctcgtgctg ggccgcctaa aactggggca agggaaagag
     1861 ttcgtcgaga gcctgccgaa gtgtgcacag cttatcaacg aagccgatgg cattctgtct
     1921 gaggccttga cctacacacg caccttggtc gcggaactca gcccacccgt gctgcgcgat
     1981 cacggtttag cggcgggcct cacctggctc ggtgcgtaca tgcagaagca tgacctcgcg
     2041 gtcacggtaa cggtcccgga ggccactctc cagttgcccg aggatcaggc gctgctcttg
     2101 ttccaatcgg ttcgggagtt gctgatgaat gcttggaagc acgcggcaac gggcaaggca
     2161 gatgtcacca tggagacaga cgacaatcga cttcgagtac aagttcacga tgacgggcga
     2221 ggctttgaag tggctgttgc tgaaactacg actgcacaca agctcgcgtc aaaattcggc
     2281 cttttcagta ttcgcgagcg gatgaaagcc ctgggtggct cgtttgagat cgaatccgct
     2341 cctcatcagg gaaccacagc tacactaacg ttaccgttgg gtgcagcatc gagcgagacg
     2401 gaagtggagc cgagaccctt tgaggtttcg caggacgggt cagagacgcc gaatgctagc
     2461 cctctaccat caggagcaca aacacgaacc gtcggtgtgt tattggtcga tgatcatgcc
     2521 atggtccgcc aagggctgcg gatgatgctg gaggactatc cagacgtgga agtcgttggg
     2581 gaggcgtgcg acggagaaga ggcgttggtg tgtaccgagc agttgcatcc agcggtggtg
     2641 gtcatggaca tcaatatgcc gaagatgaat ggcatcgagg cgacggctta cctgaagacc
     2701 cggtatcccc acatcgccat catcggcttg tcggtcaacg caggggagga aaaccagaaa
     2761 gcgatgaaga gggctggggc cacgtcgctg attacgaagg aagcggccgt ggaccaacta
     2821 tatgccgcca ttcaggcgac ttctgctcca acacgatcat gact
//
LOCUS       DASTQY010000490         7861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_722931, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000490 DASTQY010000000
VERSION     DASTQY010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722931"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /gene="infC"
                     /locus_tag="VFR82_15660"
     CDS             <1..199
                     /gene="infC"
                     /locus_tag="VFR82_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU4503087.1"
                     /translation="QIKGFLEEGNKTKVVLTYRGREMANQEMGRVVMNSVIAELSETG
                     TIEYAPRMEGRSLIMIVAPKN"
     gene            231..425
                     /gene="rpmI"
                     /locus_tag="VFR82_15665"
     CDS             231..425
                     /gene="rpmI"
                     /locus_tag="VFR82_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU4503088.1"
                     /translation="MKMKSHSGASKRFHKTGTGKFMRKRAGKRHILTSKNRERKRRLS
                     GSVVVDETKAAALKRLLPYA"
     gene            461..829
                     /gene="rplT"
                     /locus_tag="VFR82_15670"
     CDS             461..829
                     /gene="rplT"
                     /locus_tag="VFR82_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU4503089.1"
                     /translation="MPRAKGGPKTRARRKKRLKLAKGQYGAKSRLFRSATESVDKGQQ
                     YAYTGRKNRKRDFRQLWIARISAATRAHGLTYGRFINALKKANVLLDRKVLSDMAIKD
                     VAGFEQLVGLAKQHLTPATA"
     gene            complement(880..2601)
                     /gene="pheT"
                     /locus_tag="VFR82_15675"
     CDS             complement(880..2601)
                     /gene="pheT"
                     /locus_tag="VFR82_15675"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU4503090.1"
                     /translation="MPTISIYKDDLESLLAADGRAKRPLTIPQLEEWLMLVKGELKEH
                     VAETGELRIELQDSNRPDLWCCEGIARQIRIKQQRKVRPYPFFSTKSKKTLRRLNVAP
                     GLERVRPYVAACASTGYRVTEEGLAQLIQTQEKLSDIFGHKRKTVSIGLYRLARIQFP
                     VTYELVKPDAAKFTPLGMETTMTLGEILMVHPKGLEYGHILAEHEQLPILRDATGQPL
                     SFPPIINSREIGEVQVGDDALFVEVTGTDLPMVVLTLNIFAANLADRGAVIEPVDVRY
                     PTKTPFGKQVVTPRDLDITRTIPIQTIEQALGQPIGVQAVKQALEVYGYVVAPAKGTV
                     KAKLPPYRQDLMHPMDVVEDVAISRGYGDFSPVMPAQFTVGALSKIEQTSDRARELMV
                     GMGFQEIISNILGSPEQYRDAMRLNQTLWGEMVEVENVMSLSFSCLRQWMLPSLLRVE
                     AASSRAFYPHRLFESGDVAIPDRSHELGSRTETVVGAAIAHAAAHFSEIHSCLDILFY
                     HLGRDYTLEPVQHPSFLEGRAGQIVVSGKSLGIIGEVHPEVLERWQIAVPVAAFDVNL
                     SRLIEIH"
     gene            complement(2605..4170)
                     /locus_tag="VFR82_15680"
     CDS             complement(2605..4170)
                     /locus_tag="VFR82_15680"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF003210.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU4503091.1"
                     /translation="MDISSLIDSLHPLEIKVLTAFGANPSGLVLDTDQLAKATNLEAS
                     QLSMAIEWLLAKSLLAVDKETVTPVVSLTPVGERYHGKQAPIEEVLAAAKSAAGTGGR
                     LTIQDLQSQEGLDPSDVSKAVGRLKKEGALLIVQGGCLESTGRPSPTADRLRSLLQQV
                     HEGPRELESFEEHDRQIIQEYAVKRGNAKEPFRVDDRVARSFRVASAGLTASEHLAQH
                     EVAEEVSQLSPEMLKDGSWRTKRFRKYTISLRAPRISTGKRHPYREFLDTVKSKLVSM
                     GFQEMRGALVETEFWNMDALFMPQFHPARDIHDVYFVKHPTRATHVAEPFLSRVAQAH
                     RNGGNTGSMGWRYAFDVERAKRLVLRSQGTAVSARTLASGPATPAKYFSIARCFRYDQ
                     VDATHATDFFQVEGIVLGDDINFRTLLGLLNLFAREVAQAKEVKFLPAYFPFTEPSVE
                     LHVRHPRLGWMELGGAGLFRPEVTLPLGVKVPVIAWGLGLDRMAMVALGIHDIRELFT
                     NNLDMIRTTRGVF"
     gene            complement(4179..4862)
                     /gene="rpe"
                     /locus_tag="VFR82_15685"
     CDS             complement(4179..4862)
                     /gene="rpe"
                     /locus_tag="VFR82_15685"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU4503092.1"
                     /translation="MTARTALIAPSILSADFARLADEVAAVERGGADLLHVDVMDGHF
                     VPNLTVGPPIVESLKKVTKLPLDVHLMITNADAFLADFASAGADYLTVHVEACPHLHR
                     TVQAIKERGVKAGVTLNPATSLHTIEEILPDIDLLLIMSVNPGFGGQTFIESCLHKVS
                     TARRMLDGIGSRALLEVDGGVKIDNAARILAAGADVLVAGSAIFCGADYAATIAALRA
                     ARQPAPVHR"
     gene            complement(4859..6250)
                     /gene="rsmB"
                     /locus_tag="VFR82_15690"
     CDS             complement(4859..6250)
                     /gene="rsmB"
                     /locus_tag="VFR82_15690"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU4503093.1"
                     /translation="MPIHSARSVALSVLVESEHSEEGLDTLFDRALLRTPLDSRDRAL
                     ALEITYGVCRRLLTIDWRLEPVLDKPLPRLPIVVQMLLRMGAYQVLFLDRIPASAAVN
                     ETVNLVKAQAKKLRRDWGGFVNAVLRALIREPSAPWPSVHDDAAYALAVRYAVPEWLS
                     RRWLDRLGLSAAQTVCEQASLIPPMTLRVNLLQITREELLRRLEKAGIHGKPTRISPA
                     GITLVEGGSVAMLPGFAEGQFYVEDEAAQLIPFLLDVRPGNLVLDACAAPGGKTTHIA
                     ELMEDQGRIYAVDRSAGRLSVLQTNCSRLQLKSIAPIVGDLRDSSWGDAAAKADKTAD
                     RLWFDRILVDAPCSGLGVLRRHPESKWRKEQKAFARHQAVQSQILDSAARCLRAGGVL
                     VYSTCSTEPEENEAVIDGFLRSHAEFRRVSVAPWLPESGRHLITAQGDFSTMGNVDSM
                     DGFYAARLTRMDS"
     gene            complement(6388..7329)
                     /gene="fmt"
                     /locus_tag="VFR82_15695"
     CDS             complement(6388..7329)
                     /gene="fmt"
                     /locus_tag="VFR82_15695"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU4503094.1"
                     /translation="MRIVFMGTPEFSVPSLEALLKANHQVVGIVTQPDRPKGRGQVLT
                     PSPVKLIAHRERIPLLQPTKMKDVDFLATLSSWKPDYIAVAAFGRILPPAILSLPPFG
                     CINVHGSLLPKYRGAGPIQWALINGETETGITTMLMDEGMDTGAVLLQETMTINPEDT
                     AGSLSVRLAELGGQLLVKTLAQLGAGTLIPRAQDHSQATLAPLLKKEDGIIDWTMPAT
                     AIANRIRGLDPWPGAYTVAKTERWTIYRAVALDEPARGVPGEVTAVQKDAIHVATGRG
                     VLAIYEVQAAGGRRMPMAQYLTGHPIGADMRLSAHTQ"
     gene            7413..7489
                     /locus_tag="VFR82_15700"
     tRNA            7413..7489
                     /locus_tag="VFR82_15700"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7447..7449,aa:Met,seq:cat)
BASE COUNT         1751 a         2242 c         2208 g         1660 t
ORIGIN      
        1 acagatcaaa ggcttcctgg aggagggcaa caagacgaag gtcgtcctga cgtatcgggg
       61 gcgagaaatg gccaatcagg aaatggggcg ggttgtcatg aactcggtaa ttgcggagct
      121 gtctgagacc ggtacgatcg agtatgcgcc gcggatggaa gggcgcagct tgatcatgat
      181 cgtcgctccc aagaattaga ggccgaacgt ttaaccaaag gatgcacgca atgaaaatga
      241 agtcacatag cggagcaagc aaacgatttc acaagacggg cacgggcaaa ttcatgcgca
      301 aacgcgccgg caagcgccat attctaacca gcaagaatcg ggagcggaaa cgccggctga
      361 gcggatcggt ggtcgttgac gaaaccaagg ccgcggcgct caagcgccta ctgccctacg
      421 catagagatt tcaacctgag ctcaacaagg agtatgagtc atgccacgcg caaaaggtgg
      481 gccaaaaaca cgagcaagac ggaaaaaacg actcaaacta gcgaaagggc aatacggcgc
      541 aaagagccgg ctgtttcgct cagcgacaga atcggtagat aagggacagc agtatgccta
      601 caccgggcgc aagaatcgga agcgggactt ccggcagttg tggattgctc gcatcagcgc
      661 ggccacgcgg gcgcatgggc tcacgtacgg ccgcttcatc aatgcgctta aaaaagccaa
      721 cgtgttgttg gatcgcaagg tgctatccga tatggcgatc aaggatgtcg cgggatttga
      781 gcagctcgtc ggactggcca agcaacatct gacaccggcg accgcctagc ttcacctttt
      841 cgcaactcca ccagcggagg gggatgagca tccctccgct caatggatct caatcagtcg
      901 agacagattg acgtcgaagg cggcgaccgg taccgcgatt tgccaccgtt caagcacttc
      961 ggggtgcact tctccgatga tacccagcga ctttcccgac acaacaatct gccctgcccg
     1021 gccttccagg aaagacggat gttgaaccgg ctcgagagtg taatcccttc ccaaatgata
     1081 gaacaggata tccaagcagg aatgaatctc cgaaaaatgc gccgccgcat gggcgattgc
     1141 cgcgcccacg accgtctccg tacgtgaacc caattcgtgg ctgcgatccg gaatcgcgac
     1201 gtcgcccgat tcaaagagac gatggggata aaacgcccgg cttgaagcgg cttcgacacg
     1261 caacagggag ggcagcatcc attgacggag gcaagaaaaa ctcagtgaca tgacattctc
     1321 gacctcgacc atttcacccc aaagcgtttg gttcaaccgc atcgcatcgc gatattgctc
     1381 cggtgatccc aaaatgttcg agatgatctc ttgaaatccc atgccgacca tcagttctct
     1441 cgcccggtcg gatgtttgct cgattttcga gagcgcgccg acggtaaact gtgcgggcat
     1501 gacgggggag aagtcgccat aaccccggct gatagcgacg tcctccacga catccatggg
     1561 atgcatgagg tcctgtcgat agggcggtaa cttggcctta accgtcccct ttgccggtgc
     1621 gaccacatac ccatacacct cgagggcttg tttgaccgcc tgcacgccaa tcggctgacc
     1681 gagcgcctgt tcaatggtct ggatgggaat cgtccgcgtg atatcgaggt cccgaggcgt
     1741 cacgacctgt tttccgaacg gcgtcttggt gggatagcgc acgtcgaccg gctcaatgac
     1801 cgcacctcgg tcagccagat ttgctgcgaa gatattcagc gtcaacacta ccattgggag
     1861 atcggtcccc gtgacctcca cgaacaaggc atcgtcaccg acttgcactt cgccgatttc
     1921 gcgactgttg ataataggcg gaaatgataa cggctgccct gtggcgtctc gaagaatcgg
     1981 caactgctcg tgctcagcta agatgtgtcc atactcgagc cctttcggat ggaccatcag
     2041 aatttcgcct agcgtcatcg tcgtctccat gccgagagga gtgaacttcg cggcatccgg
     2101 tttcacgagt tcatacgtca cagggaattg aatacgcgct aaccggtaca gaccgatgga
     2161 gacagttttc cgcttgtgtc cgaagatatc ggacagtttt tcctgcgttt gaatgagctg
     2221 cgccaatcct tcctccgtca cgcgataccc cgtggatgca caggctgcaa cgtacgggcg
     2281 cacccgctcc aggcccggcg cgacattcaa gcgtcttaag gtcttcttgg acttagtcga
     2341 gaagaacggg tagggcctga cctttcgttg ttgtttgatg cggatttgcc gtgcaatccc
     2401 ttcgcaacac cagagatcgg gccggttgct gtcctgtagc tcgatccgta attctcccgt
     2461 ctctgcgaca tgctctttca actcgccctt caccagcatc agccattctt cgagctgcgg
     2521 gatagtcagg ggccgtttcg cacgtccatc cgccgcaagc agtgattcca aatcgtcttt
     2581 ataaatcgaa atcgttggca tgagctagaa cacgcctcgt gtcgttcgga tcatatccag
     2641 attattcgta aagagctcac ggatatcgtg tatgcccagg gcgaccatcg ccatacgatc
     2701 cagaccaagc ccccaagcga tgaccggcac cttgaccccg agcggcaggg tgacttccgg
     2761 cctgaagagt cctgcaccgc cgagttccat ccaaccgaga cgcggatgac ggacatgcaa
     2821 ttcaaccgag ggctccgtga acggaaaata cgccggcaag aactttactt ctttggcctg
     2881 tgccacttct cgagcaaaca gattcaacaa cccgagcaga gtgcggaagt tgatatcgtc
     2941 tccgaggaca atcccttcga cctgaaagaa atccgtggca tgggtcgcat cgacctggtc
     3001 gtaacgaaag cacctcgcga tggaaaaata tttggccggt gttgccggcc cagacgccaa
     3061 ggttctcgct gacactgccg taccttggct cctcaatacc agccgcttcg cccgttcgac
     3121 atcaaaggcg taccgccatc ccattgaccc ggtgtttccg ccgtttcgat gagcctgagc
     3181 gacgcgagaa aggaacggtt cagcaacatg cgtggcacgg gtgggatgct tgacgaagta
     3241 cacatcgtgg atgtcgcgtg ccggatggaa ctggggcatg aaaagtgcgt ccatgttcca
     3301 gaattccgtc tcgaccagcg ccccccgcat ttcctggaac cccatgctca ccagctttga
     3361 tttgacggtg tccagaaact cgcgatacgg atgtcttttc cctgtgctga tccgcggcgc
     3421 gcgaaggctg atggtgtatt tcctgaaccg ctttgtacgc caactgccgt ccttgagcat
     3481 ttcgggactg agctgggaga cttcttccgc aacttcatgt tgcgccagat gctccgatgc
     3541 cgtgaggccg gctgacgcca ccctgaaaga tcgagccaca cggtcgtcga cgcgaaacgg
     3601 ttctttcgcg ttgccccgct tgacggcgta ctcttggatg atctgccggt cgtgttcttc
     3661 gaaactttct aattcgcgag gaccttcatg cacctgctgc agtaagcttc tcagccgatc
     3721 ggccgtgggg ctcggacgtc cggtggactc aaggcaacct ccctgcacaa tgaggagcgc
     3781 cccttccttt ttcaggcggc cgacggcctt actgacatcg gatggatcga ggccttcttg
     3841 agactgcaga tcctggatcg tcagccggcc gccggtccct gctgcactct tcgccgccgc
     3901 caagacttcc tcaatcgggg cttgctttcc atgataacgc tcgccgacag gtgtcagcga
     3961 gacgactggg gtaacggttt ccttgtcgac agcgagcagc gatttggcaa gcaaccattc
     4021 gatggccatg ctgagttgcg aggcttcaag atttgtggct ttggcgagtt gatcggtgtc
     4081 gaggaccagc ccggatggat tggcgccaaa ggctgtgagc accttgattt ccagtgggtg
     4141 caagctgtcg atgagggagg aaatgtccac ccgatggcct atcgatggac gggtgcaggc
     4201 tgccgagccg cccggagagc agcgatcgtc gccgcataat cggctccgca gaaaattgcc
     4261 gaaccagcga ccaacacgtc tgcgccggcg gcgaggattc gagccgcgtt gtcgattttg
     4321 acgccgccat cgacctcgag cagcgcacgg ctcccgatac cgtcgagcat gcgccgtgct
     4381 gtgctcacct tgtggagaca ggactcgatg aacgtctgtc caccgaatcc gggattcacg
     4441 gacatgatca ggagaagatc gatatccgga agaatttctt cgatggtgtg caatgaggtt
     4501 gcgggattca acgtgacacc cgccttgacg ccacgctcct tgatggcttg caccgtccgg
     4561 tgaagatgtg gacaggcttc gacatggacg gtcaggtaat cggcaccagc cgaggcaaag
     4621 tcggcaagaa aggcatctgc gttcgtaatc atcaggtgaa cgtcgagggg aagcttggtc
     4681 actttcttca aggattccac gatcggcggg cccaccgtca ggttcggcac gaaatggcca
     4741 tccatgacgt caacgtgcag cagatctgct ccacctcgct cgaccgcggc cacttcgtcg
     4801 gccaggcggg caaagtcggc agagagaata gaaggagcga tcaacgcggt tctggcggtc
     4861 atgaatccat tcgtgtcaag cgtgctgcat aaaagccgtc cattgaatcc acgtttccca
     4921 tcgtcgagaa atcgccttgt gcagtgatga gatgccggcc ggattccggc agccagggtg
     4981 ccacggacac tcgacggaac tccgcatgcg acctcaggaa tccgtcgatg acagcttcgt
     5041 tttcctccgg ctctgttgaa cacgtactat agaccagcac cccgccggcc cgcaagcatc
     5101 gagccgccga gtcgagtatt tgcgattgca ccgcctgatg ccgtgcgaac gctttctgtt
     5161 cctttcgcca ttttgactct ggatgccgtc gcaacacgcc gagaccgctg catggggcat
     5221 cgacaagaat tcgatcgaac cataagcgat cggccgtttt gtccgctttg gcagctgcat
     5281 ctccccacga agagtctctc aggtcgccaa cgatcggcgc aatgcttttt aattgtaatc
     5341 ggctgcaatt tgtctgcaag acggagagcc tgccggcact gcgatcgact gcgtaaatcc
     5401 gtccctggtc ttccatgagt tcggctatgt gcgtcgtctt ccctccaggc gcggcgcatg
     5461 cgtccaggac aaggtttccg ggacgcacgt ccagaaggaa agggatcagc tgggcagcct
     5521 cgtcctcgac atagaactga ccctctgcaa aaccgggcag cattgccacg cttcctcctt
     5581 ccacaagcgt gatccctgcc gggcttattc gcgtcggctt tccgtgaatg ccggccttct
     5641 ccaatcgccg gagcaattcc tcccgagtga tctgcagtag attcacgcga agggtcatcg
     5701 gaggaatgag gctcgcctgt tcgcagacag tctgagcagc ggataggcca agccgatcga
     5761 gccagcgacg actcaaccat tcgggaacag cataccgtac ggcgagtgcg tacgcggcat
     5821 catcgtggac tgagggccat ggcgcgctcg gctcgcgaat cagcgcacgc aagacggcat
     5881 tgacgaaacc accccaatcc cgccgcaact tcttcgcctg ggccttgact agattcacgg
     5941 tttcattgac agcagcggaa gcggggatcc gatcgagaaa aagcacctga taggcaccca
     6001 tccttaacaa catctggacc actatcggca aacggggcag cggcttatca aggaccggtt
     6061 ctaaccgcca atcgatagtg agcaagcggc ggcagacccc gtatgtgatt tcaagggcca
     6121 acgcacggtc gcggctatca agcggtgtgc ggagcagcgc acgatcgaaa agtgtatcga
     6181 ggccttcctc cgaatgttct gattcaacga gcaccgacaa tgcaaccgac ctggcagaat
     6241 ggataggcat gccaattgag aacagtggca ctagcagaaa aagcccgcgg gaacgccgcg
     6301 gagcacaaat tgatcaatcg tctggcatga gcggctgttg catttgccag ggactgactg
     6361 gcattcactg atgagagtat cgagcgttca ctgtgtgtga gcgctcagtc gcatgtcggc
     6421 accgatcgga tggcccgtta gatactgcgc catcggcatt cgacggccac cggccgcttg
     6481 aacttcgtag atcgccagta cccctcggcc tgtggccaca tggatcgcat ccttttggac
     6541 tgctgtcact tcaccgggaa cccctctcgc cggctcgtct aaagccaccg cccggtaaat
     6601 cgtccagcgc tccgttttgg caacagtata ggcaccaggc catggatcga gcccgcggat
     6661 acgattggca atggcagttg caggcatcgt ccagtcgatt atgccatcct ctttcttcaa
     6721 aagtggagcc agagtcgctt ggctatgatc ttgagctcga ggaatcagag taccggctcc
     6781 taactgggcc agagtcttca caagcagttg tcctccaagc tcagcgagac gaacagacaa
     6841 gctgccggca gtatcttcgg ggttgatagt cattgtttct tggagcaaca cggctccggt
     6901 gtccatgccc tcatccatca acatggtcgt aataccggtt tcggtttccc cattgatgag
     6961 ggcccattgg atcggacctg ctccccggta cttcggcaac agtgatccgt gcacattgat
     7021 acagccaaag gggggaaggg aaaggattgc gggaggcagg atacggccga acgctgcgac
     7081 cgcgatatag tctggtttcc aactggataa tgtcgctaaa aagtctacat ccttcatttt
     7141 tgtcggttgc agaagcggta tgcgctcacg atgtgcgatg agtttcactg gagagggcgt
     7201 taatacttga ccgcgtcctt tcgggcgatc gggctgcgtc acaatgccca caacctgatg
     7261 gttcgctttg agcagcgcct caagtgatgg aacggagaat tctggtgtcc ccatgaacac
     7321 tatgcgcatg caccgcttta gcattcgtga ggcacgaaag caacgggact gcatggtttg
     7381 acttgccgag aatcgctttg ttagtatccg cgcgcggggt ggagcagtct ggtagctcgt
     7441 tgggctcata acccaaaggt cagaggttca aatcctctcc ccgcaaccaa tacttttcaa
     7501 ggatttcgta taccccttca aaagatgaac ggttgaagcc tacgtgattt ctgggtttca
     7561 gtccgtccct tcctcacaga ggctttctta tgtatctggc ttacccgaag cgagaggttt
     7621 ggctcattat ttatgaattt gtcgcgagcg cctgggttga gttagggatt gatgggatcc
     7681 ctccgcctga accgccatgc tccgacgtgt ggtccatgct ctcagatagt gacaaggaag
     7741 atgcctggac tcttctgctc gagtggcttg aacaccacga cattgtagag ttactggatg
     7801 ccgtatcaga tgctgattgg tgcagggggc aagggcagta tgtcgaggag ccgacatcag
     7861 a
//
LOCUS       DASTQY010000491        12130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_173637, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000491 DASTQY010000000
VERSION     DASTQY010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 12130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12130
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173637"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VFR82_15705"
     CDS             complement(<1..496)
                     /locus_tag="VFR82_15705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEU4503095.1"
                     /translation="MIRKIVAALHPMGGRTETAYGQDRRTVPCPILKRSEHPPQNYDD
                     EPAAREAIALIENHTMLSYPRLVTLWQQVCYLDRTGIEGCLVECGTWKGGAAALMGLA
                     HRRAGEACRDLHLFDSFQGLPEPDRAHDGEMAVRYAGERATGRLQSIGKCVGTLEDNK
                     HVLTT"
     gene            complement(493..1716)
                     /locus_tag="VFR82_15710"
     CDS             complement(493..1716)
                     /locus_tag="VFR82_15710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4503096.1"
                     /translation="MTDLTSIRVDGLWKRYGLSSCAGIGQLVRAVTREFRPNTKGDVD
                     LWALQDVSFEVRRGETVGIIGRNGAGKSTLLKVLAGVTPPSRGRVEVYGRVFPMIELN
                     AGVHPELTGRENSYLLGAIMGITGAHMDRVLPAIEEFVELGDWFDRPVRTYSSGMVAR
                     LGFGVATQIQSDILLIDETFAVGDLKFQNKSLARLKELRERGATVILVTHSLDMLQFI
                     AQRGIVLDGGAIVAEGSALEGLRAYEQLMFGKERMRLEQRGRSGRPGSEVTILATRIF
                     GAGEETLTEVEAQQPFGIEIRFKMHRPIDRPVFSIGILNATGVVCVWNVSEEDGLHCE
                     AVTGVMCLRVWYAEHKLSTGAYEVNFALREHTSYETLERLGGIAAFSVTGPGRSRGIM
                     ALSAQWQLHAERREI"
     gene            complement(1719..2525)
                     /locus_tag="VFR82_15715"
     CDS             complement(1719..2525)
                     /locus_tag="VFR82_15715"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4503097.1"
                     /translation="MNGKGVYIHLEPAVEPWRQFMALMWAISLREMKGRYRRSALGIL
                     WAVLPPVFYAGLFTALQGILHIGTDDVPYVLFAYAGLVPWAFFVNAVNRCGGSVGANA
                     GIVKKVAVPRTVFPVTAVVTALADFAVSSALLAGLLAWFGIHPAVAWLWLAPLLLITS
                     LFSLGCGLIVGALGTFLQDVLFGLPLLLQLWMLATPIMYPLRQVPAGWMPLYILNPMV
                     GIIEGFRQVLIRQQAPDFELLGVSLVATACVWGIGWPLFKYLSQHFADAV"
     gene            complement(2551..3702)
                     /locus_tag="VFR82_15720"
     CDS             complement(2551..3702)
                     /locus_tag="VFR82_15720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4503098.1"
                     /translation="MNALAVLPPDRWMEINLLDTLRHDYCDHLEVFTYPGGMGRLGSS
                     TWRGHRDQLNAALVRLARTLKSANRLDFMFFIVYDDFLTVETARQLQALRVPMINYHV
                     DMAFQWYRVIRTAPYFDVLAVAQMVNADHLKPYNDHIVWMPMAANPRFYQARGAEYRH
                     GVSFVGSFNPYRRALLKACVEGGVKPVVFGRGWQPDVPNGYEFDWDWHKVVHDLRYYA
                     WPRWRAEGMSSLTGPLRRKLARRHVFHELDGPEFHLPCADEALPDIFRTSRVNLGFSD
                     TGWHTDETVLASNNLQCRLRDFEVPMSGGFYLVQQAPDHGDYYQLGREIETWSQPDEL
                     IDKALFYASHPKEAERIREAGHARVLGCHTWKHRFDRLFQREEIAKKVA"
     gene            complement(3726..4490)
                     /locus_tag="VFR82_15725"
     CDS             complement(3726..4490)
                     /locus_tag="VFR82_15725"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4503099.1"
                     /translation="MQSLLVVAQNTFRETVRDKILYNLVFFALLLIGASVLLGTLTIG
                     EQARIINDVGLAAINLVGIIIAIFVGIGLVTKEIDRRTIYTILARPITRTQFVLGKYC
                     GLVFIGGLNIAIMFAMFLSTIWLSGNVVAGALFQAVELMLVEVLLVTALAMFFSTFSS
                     PTLSAIMTLGFYVIGHLTGDLKGIAARTKNPLTESIMTVLYYVCPNLEWLNIKGQAAS
                     GVPVSVEYQVAASAYGLLYAGLLLFGACRIFERRDF"
     gene            complement(4490..5422)
                     /locus_tag="VFR82_15730"
     CDS             complement(4490..5422)
                     /locus_tag="VFR82_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4503100.1"
                     /translation="MDAIVIDQITKTHRPTWPWQQPATVLRDVSMSVGQGEIFGFLGP
                     NGAGKTTTMKIVLGLMRASSGSVELLGRPVQDVDVHKKVGYLPESPYFYDYLTAEEFL
                     SFYGRLGGGSNADLHQRIPSLLERVGLLDARCRPLRKFSKGMLQRIGLAQALIHDPEL
                     VILDEPMSGLDPLGRKDVRDIILSLRDQGKTVFFSTHIISDVEMICDRVGILAKGKML
                     AVGRIEDIVSRHATQSVEVVCEGVLGNSIPLIKDAAIRVLQRGSRCWMSLPGQQKLED
                     VLAAIRHAGGRLVSVIPHKGTLEEIFLEQTNQGI"
     gene            complement(5527..6405)
                     /locus_tag="VFR82_15735"
     CDS             complement(5527..6405)
                     /locus_tag="VFR82_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503101.1"
                     /translation="MKVWREHISFWSISVIMMGLLIALQFALRPWQEADKKLERLMYL
                     PDGEYMKIASLGYRQLLADLLWIQAIQVMGEKKVSDSNGRWLYRALDIVTTLDPQFVR
                     AYEAGGLALTTLVVLPEESNRLMKKGMQHNPTEWKLPFLIGINYYYELYDDAKAADYI
                     SQASRLPNAPSSLATLAANLYVSAHSPQQAVNLLAAMYQEATDESAKKLLEIRLKIVM
                     IERDLYVLEQAIDRYRNMKGTNPSGLDDLVSAGLLSALPVEPAGGRYAYDAKTGAVSS
                     TEMPERLTMTGRRRNR"
     gene            complement(6402..8480)
                     /locus_tag="VFR82_15740"
     CDS             complement(6402..8480)
                     /locus_tag="VFR82_15740"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU4503102.1"
                     /translation="MPDHPGHINTLPDALSVGGRVQAGAFVSHISDTTAWLKREIAAW
                     PGIALFMTIGWLVLFSPLIDGGTTQLPVLLIRLVILVSAGIWLVGRIREGSLFLPQTP
                     LAVSVALFCGWSILSITWSPYKNASVQWILNIVSYAMLFVMVTQGIRLPGQKWALVLL
                     VSGLGVSEGIWGISQYLWLGEPRAHGTFFNPNFFAAYEAAVFLLSLGVLLFSQRATLS
                     LLWASWLCGAAAVSLAAFVAAQSRGAAFALIGAISFLSFCRYGKRASAILVVCLLAGM
                     VVPNPLQNRIVHVASQDPYAYTRVEIWKSSFERLLGHPLGIGVGMYKQGSFQDRFPIE
                     GNIVRYGKRPESAHNEYLQMGVELGLVGVLVFLGGLWVWSTEVRRLCCPSADRTNRGL
                     IMGLTSSALVILLHAAVDSTFHEPALVILLILMAGLTHNLAMQSGSTTVVWRRIPFSY
                     HPLRVTAVVVGMLLIAAVCSQSAVGWYAHEEGKRHAAQQHLEQAMSWYVRAAAIDPGT
                     TGYHDSIARTALELFVESGTSDWLLRAAEEEAMARSLNEADGRFAFRLGTIYRLMAVQ
                     PGSTTQRDELLEKASGAFTDAIRLDPFSPSSYYELAQLHLAHGRTDAAIALLTTAKGY
                     EPNFLPGRALLAELSLKAGKPGDYQGEYAAIKAVQSRYAGRELNEIERRFLDVDLYPL
                     GRALAMEGVS"
     gene            complement(8688..9206)
                     /locus_tag="VFR82_15745"
     CDS             complement(8688..9206)
                     /locus_tag="VFR82_15745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU4503103.1"
                     /translation="MCKSFKGQKGFTLIELMIVVAIIGILAAIAIPNFVAYQAKSKQS
                     EAKVSLGAIFTSAVAYQAESQNPQSYSPATISQIGWQPSGSPRYSFWYQDGVNAAGTG
                     TTVTRFNGSSNAATPCNVTVAPTSGGFSVLATSSGFTAGANGNIDGDTACDHWFINDQ
                     RNLQNTNNDVSL"
     gene            complement(9492..12059)
                     /locus_tag="VFR82_15750"
     CDS             complement(9492..12059)
                     /locus_tag="VFR82_15750"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU4503104.1"
                     /translation="MVHHHAITEPDEISGGQRPAGALGPTEPSKALGGVFDAGLDRRD
                     ALCLAGLIGLLLLVYAQLWLPGLVLIKRDAFQLFLPLKQYIIDRLSAGELPEWFPYEG
                     LGRPLIALTVMGVFHPFTALYWLLPVQDAYRLSTLLSCLMAASGTFLLARVLGISRVG
                     AVIGSVGFSCSGYVMSLTENILYLYSLCTLPLFVCSIEKLMVTRTLRWIGASALIWAS
                     VILHGDIQTAYYYGGVAVIWTIMRGKQAGLFGRLIAVAALTVLVAAVQLAPSWISYQR
                     SDRTDPALFHAEAIHWSTHPLRLLTMLMSPIGDSAQGDRIAETLFHTQEQGRGPAGFW
                     AESLYLGLPVIGLGLAGLRRSGMLVVVVITVSSITLAMGSYAGVYDLFYDWVPLWSAF
                     RYPEKLMGIATFGLALLAAGGVDVLRRGRPGTCLWYLTAGLLAALAGVLGTQQGPHFF
                     SKLFLLPTDLASHISHAMAVSALYAGAAAAGMGMILRWMAIRPADQFASGAALVLLMV
                     LDLARVNLPMVQTSSSEAWTFTPGLVSALSEDAKAQGPGHFRILSIKDSMAAVSEEVE
                     KRLTSRERIAALRRNGLYLEHNAVFRIESIQHYLAGLSPRVDEIGRNASLQVAARYNV
                     AYLIGRPARFESDSFARSVVASIADYDLALVRNPVPVTPRAYLSRRPEVMAPAMPMRD
                     LLQRQEFLSAQVDGIEGAPVPLPGASPVGHASIAEYQPERVQVDVQTQGPAVLVLTDA
                     FEPGWKANIVGGDQLEIFRANGLVRAVLVPGGHNRIVFQYETPLLKVGAWLSGLGFVL
                     VTLLFCVKVKKEGVSKSVGPVSKLFPSFPPIGANGMPTGTTGTQSVLTAESGLSATE"
BASE COUNT         2757 a         3692 c         3325 g         2356 t
ORIGIN      
        1 ccgtcgtcaa cacgtgtttg ttgtcttcga gtgtgccaac gcacttgccg atgctttgca
       61 gccgtcccgt cgctctctcg ccggcataac gcacggccat ctcaccgtca tgtgcccgat
      121 ccggttcggg cagtccttga aacgaatcga ataaatgcaa gtcccggcac gcttcgcccg
      181 cccgcctgtg tgccagcccc atcagcgcag cagcacctcc cttccaggtc ccgcattcaa
      241 ccagacagcc ctcgatcccg gttcgatcga ggtagcacac ctgctgccac agcgtgacaa
      301 gacgcgggta ggacaacatt gtatggtttt caatcagcgc gatggcttcc cgggcagccg
      361 gctcgtcatc atagttctgc gggggatgct cgcttcgctt gaggatggga cacggcacgg
      421 tgcgtcgatc ctgcccatat gccgtttccg tgcggccgcc catgggatga agcgcggcga
      481 caattttccg tatcatattt ctctccgttc agcatggagt tgccactgag ccgaaagggc
      541 catgatgccg cgcgaccgtc cgggacctgt gacggaaaat gcggctatgc cgcccaggcg
      601 ctcgagcgtc tcatatgaag tgtgctctcg aagcgcgaaa ttgacctcgt aggcgcctgt
      661 agataatttg tgctcggcgt accacactcg taggcacatc acgccggtca ccgcctcgca
      721 atgcaacccg tcctcctccg agacattcca gacgcacacg accccggtcg cgttgaggat
      781 gccgattgaa aagacgggac ggtcgatcgg acgatgcatt ttgaacctga tctcgatgcc
      841 gaacggctgc tgcgcttcga cctccgtgag ggtctcctcg cctgcgccga aaatccgggt
      901 ggccaagatt gtcacctcgg atcctggacg gcccgatcga ccgcgctgct cgagccgcat
      961 gcgctcctta ccgaacatca gttgctcata cgctctcaac ccttccaagg cagagccctc
     1021 cgcgacgatg gcacccccgt cgagcacaat cccgcgttgc gcgatgaact gcagcatatc
     1081 gaggctatga gtgacgagaa tcaccgtcgc gcctctttcc cgaagttctt tgaggcgggc
     1141 aaggctcttg ttttgaaact tcagatcccc gaccgcaaat gtttcgtcga tgagcaggat
     1201 gtcgctttgt atttgagtgg ccaccccgaa gcccaaccgc gccaccatcc cgctcgaata
     1261 ggtgcggacc gggcggtcaa accagtcccc caactcgaca aactcctcga tcgcgggcag
     1321 cacacggtcc atgtgggcgc cagtgatgcc cataattgcg cccaagagat agctgttctc
     1381 gcggccggtg agttcgggat gcacgccggc attgagctcg atcatgggaa agactcggcc
     1441 gtacacttcc actcgtcccc ggctcggcgg tgtgacgccg gccagtactt tgagcaacgt
     1501 gctcttgcct gccccattgc gtccgatgat accgaccgtt tcacctcttc tcacttcgaa
     1561 cgacacgtcc tgaagggccc aaaggtcgac gtctcccttc gtgttcggcc gaaattctct
     1621 ggtcaccgcc cggacaagtt ggccaatgcc ggcacaggac gacagtccat agcgtttcca
     1681 caacccgtcc acccgaatgc tggttaaatc tgtcatcgtc agacggcatc cgcaaaatgt
     1741 tgggacaggt atttgaacaa cggccagcca atcccccata cgcaagccgt ggccacgagg
     1801 gacaccccaa gcagctcgaa atcaggtgct tgctggcgta tcagcacctg cctgaatccc
     1861 tcaatgatcc ccaccatggg attcaaaata tagagaggca tccaaccagc cggaacctgc
     1921 ctcagcggat acatgatcgg cgtcgcgagc atccaaagct gcagtaatag cggcaagcca
     1981 aacagcacat cctgcaggaa ggtgcccaac gcccctacaa tcagaccgca ccccagcgaa
     2041 aagaggctcg tgatcagcaa aagcggagcc aaccacagcc aggcgacggc cggatggatg
     2101 ccaaaccacg ccaagaggcc tgcgagcagg gcgctggaga cggcgaaatc ggccagggca
     2161 gtcaccaccg ccgtgactgg aaacaccgtg cgcggaacgg ccactttctt cacgataccg
     2221 gcgttggctc cgacactccc gccgcagcga ttgactgcat tcacaaaaaa cgcccaggga
     2281 acaagcccgg catacgcaaa gaggacgtag ggcacgtcgt ccgtcccgat atggagaatg
     2341 ccttgaagcg cggtaaacaa ccccgcgtag aagaccggcg ggagaaccgc ccacaatatg
     2401 ccgagggctg aacgacgata gcgtcccttc atttcccgca acgaaatcgc ccacatcagc
     2461 gccatgaatt gccgccaagg ctccacagcc ggctcaagat gaatgtagac gccttttccg
     2521 ttcatggtgg ttgcccttta gcgcccgcag ttaggccacc ttcttggcaa tctcctctcg
     2581 ctgaaagagc cgatcgaatc gatgcttcca tgtgtggcat cccaggaccc gggcatgtcc
     2641 ggcctcccga atgcgctccg cctctttcgg atgcgaagca tagaacaagg ccttatcgat
     2701 tagttcatcc ggctgagacc aggtctcgat ttcgcgtccg agctggtaat agtcgccgtg
     2761 atcgggcgcc tgctgaacca ggtaaaatcc gccggacatc ggcacttcga aatcccgtaa
     2821 ccggcactgc agattgttcg aggccagtac ggtctcgtcg gtgtgccatc cggtatctga
     2881 gaatccgagg ttgacgcggc tggttcggaa gatgtctggc agcgcctcat ccgcgcaggg
     2941 caaatgaaac tcaggcccgt caagctcgtg aaagacatga cggcgagcga gttttcgccg
     3001 aagagggccg gtcaggcttg acatgccttc ggcgcgccac cgcggccagg cataataccg
     3061 caggtcgtgc acgaccttat gccaatccca gtcaaactca tagccgttcg gcacgtcggg
     3121 ctgccagcct cggccgaaca cgactggctt cactccccct tcgacacagg ccttcagtaa
     3181 ggcccgccgg taggggttga agcttcccac aaaagacacg ccgtgtcggt actccgctcc
     3241 gcgagcctga taaaatctcg gattggcggc catgggcatc cacacgatat ggtcgttgta
     3301 cggcttgaga tggtctgcat tgaccatctg tgcaaccgcc aatacatcga agtacggtgc
     3361 cgttctgatc acccggtacc attgaaacgc catatccacg tgatagttga tcatcggcac
     3421 tcgtaaggct tgaagctgcc gcgccgtctc cacggtgaga aagtcgtcgt agacgatgaa
     3481 gaacatgaaa tccagccggt ttgctgactt gagtgtcctc gccaagcgaa cgagcgctgc
     3541 atttaactgg tcccggtgcc ctcgccacgt gcttgagcct aaacggccca tcccacccgg
     3601 atacgtaaac acttcgagat gatcgcaata gtcatgacgc aacgtatcga ggagattgat
     3661 ctccatccac ctatcgggcg gtaatacggc aagtgcgttc atggtctaat ggaccggaat
     3721 gctccctaga agtctcggcg ttcgaatatg cggcaggctc cgaacagtag cagtccggca
     3781 tacagtagtc cgtaggcgct ggcggctact tgatattcca cgctcaccgg cacgccgctg
     3841 gcggcctgcc ctttgatgtt caaccactcc agattgggac atacgtagta cagcaccgtc
     3901 atgatacttt cggtgagggg attcttcgtc ctggcggcga tgcctttcag atcccccgtg
     3961 agatggccga tgacgtagaa acccaacgtc atgatggcgc tgagcgtggg agaactaaac
     4021 gtggaaaaaa acatggctaa ggcggtgacg agcaggactt cgacgagcat gagctccact
     4081 gcctgaaaca gcgcgccggc gaccacgttc ccgctcaacc agatcgtgga gaggaacatc
     4141 gcaaacatga tcgcgatgtt cagacctccg ataaacacga gcccacaata ctttcccagc
     4201 acaaattgcg tccgtgtgat cggccgcgcg agaatcgtat aaatggtgcg gcggtcaatc
     4261 tctttggtca ccaggccgat gcccacgaaa atggcaatga tgatgccgac taggttgatg
     4321 gcggccagcc ccacgtcatt gatgatgcgc gcttgctctc caatggtgag cgtaccgagg
     4381 agcaccgagg ccccaattag gagcagggca aagaacacga gattgtagag aatcttatcc
     4441 cgcacggttt cacggaacgt gttctgagcc accaccagca ggctttgcat cagattcctt
     4501 ggttcgtttg ctccaggaaa atttcttcga gcgtgccctt atggggaatg accgacacca
     4561 accggccgcc ggcatgacga atggcggcca gcacgtcttc caatttctgc tgccccggga
     4621 gagacatcca acagcggctg cctcgttgca acacgcgtat cgccgcgtcc ttgatcaagg
     4681 ggatgctgtt tcccaagacg ccttcacaga cgacctcgac ggactgcgtc gcatgccggc
     4741 tgacaatgtc ctcgattcga cctacggcca gcatctttcc cttcgcgaga attccgactc
     4801 gatcgcaaat catttcgacg tcggagatga tatgcgtgct gaaaaagaca gttttcccct
     4861 ggtcacgaag actcaggatg atgtccctca cgtcttttcg tcccaacgga tcgagtcccg
     4921 acatcggctc gtctaggata accagctccg gatcatggat cagcgcctgc gcaagaccaa
     4981 tgcgctgcaa catccccttg gaaaattttc tcagtggacg acaccgtgcg tccaataaac
     5041 cgacgcgctc caagagggag ggaatccgct ggtgaagatc cgcgttgctc ccaccgccga
     5101 gcctgccgta aaacgagagg aattcttcag cagtgagata atcgtaaaag tacggtgatt
     5161 caggcagata gccgaccttc ttgtgcacgt ccacatcctg gaccggccga ccgagcagct
     5221 cgactgatcc gctactcgcc cgcatcaaac ccaggacgat tttcatcgtc gtagtcttgc
     5281 ctgctccgtt cggtccaaga aacccgaaga tctccccctg tccgaccgac atcgatacgt
     5341 cccgcaggac agtcgcaggc tgttgccacg gccaggttgg ccggtgcgtc ttggtaatct
     5401 ggtcaataac gatggcatcc atgtgatttc tctagtattt tacgaattcg ctcctgtcga
     5461 cgacgatcat agcagggcag cgccaatgag caagcattcg cacgattcac tccatgggga
     5521 cgctcatcag cgattccttc ttcttccagt catggtcaat cgttcaggca tctcggtgct
     5581 cgaaaccgcc ccggtcttcg catcataggc atatcgcccg ccggccggtt ccactggcaa
     5641 ggcagagagc aaaccggctg agacgaggtc atccaggcca gacggattgg tgcctttcat
     5701 gttccgatag cgatcgatcg cctgctccag cacgtagagg tctcgctcga tcatgacgat
     5761 ctttaaccgt atctcgagca gcttcttcgc gctctcatcc gttgcctcct ggtacatggc
     5821 tgccagcaga ttgacggctt gctgaggtga atgggcggag acatagaggt ttgcggccag
     5881 cgtggccagg ctcgatggag cgttcggcag gcgcgaggct tgggagatat agtcggcggc
     5941 tttggcatcg tcataaagtt cgtagtagta gttaatgccg atcaggaatg ggagtttcca
     6001 ttccgtcgga ttgtgctgca tgcctttttt catgagccga ttactctctt cgggcaggac
     6061 gacgagcgtc gtcagtgcga gccctccggc ctcataggcc cgcacgaact gcggatccag
     6121 ggtcgtcacg atgtccaacg cgcggtacaa ccaccggccg ttcgagtcag agaccttctt
     6181 ctctcccatc acctgaatcg cctggatcca tagcaggtcg gcgagcagct gccggtagcc
     6241 caacgaggcg atcttcatat attcgccgtc gggcagatac atgagtcgct caagtttctt
     6301 gtcggcttcc tgccatggcc tcagcgcgaa ctgcagcgca atgagcagtc ccatcataat
     6361 cacgctgatc gaccagaaac tgatatgctc acgccaaact ttcatgacac cccttccatg
     6421 gccagcgctc tgcccagtgg atacaggtcg acgtccagaa agcgtcgctc gatctcgttg
     6481 agctctctgc ccgcatacct cgactgcacg gctttgatgg ccgcgtactc cccctgatag
     6541 tcgccgggtt tgcctgcctt caaggacagc tctgccagca aggcacggcc tggcaggaaa
     6601 ttcggttcat atcctttcgc cgttgtgagt aaggcgatgg ccgcatcggt acggccatga
     6661 gcaagatgca attgggcgag ttcgtaatag ctggacggcg agaagggatc caaacggatc
     6721 gcatctgtga acgctcctga ggctttctcc aacaactcgt cacgctgggt cgtgctgcca
     6781 ggctgcaccg ccatcagccg gtagatggta cccagtcgga acgcaaatct tccgtcagcc
     6841 tcgttcagac ttctggccat agcttcttct tctgccgccc ggagcagcca gtccgaagtt
     6901 cccgactcga cgaacagctc gagggccgtt cgagcgatcg agtcatgata gccggttgtt
     6961 cccggatcga tggcggcggc acgaacgtac cagctcatcg cctgttccaa atgctgttgc
     7021 gccgcatgac gcttgccctc ttcatgggca taccaaccca cggcggattg cgaacagacg
     7081 gcagcgatca acagcatgcc gaccaccacc gctgtgacac gcaaaggatg gtagctaaag
     7141 gggatgcgtc gccacacgac ggttgtagag ccggattgca tggcgagatt gtgggtaagt
     7201 ccagccatga ggatgagcag gataaccagc gccggctcgt gaaacgtgga atctacggcc
     7261 gcatggagca agatcacgag cgcagagctt gtgagtccca tgataagccc tcgattcgtc
     7321 cggtcagccg atggacagca cagccgccgg acttcggtgg accaaaccca aaggccaccc
     7381 agaaacacca agacgcctac caaccccagt tccactccca tctggagata ttcgttgtgc
     7441 gccgactcag gccgtttgcc atagcggacg atattccctt cgatcggaaa ccgatcttga
     7501 aacgatccct gcttgtacat gcctactcca atgcccagcg gatgccccaa caggcgctca
     7561 aaagagcttt tccagatttc caccctggta taggcgtagg gatcctgcga agctacatgc
     7621 acgattcgat tttgcagcgg attcgggaca accatccctg cgagcagaca aacaaccagg
     7681 atcgccgagg ccctctttcc atatcggcag aagctgagga acgatatcgc tccgatcagt
     7741 gcaaaggccg ctccccgtga ctgcgcggca acaaatgcgg ccaatgagac cgccgcggca
     7801 ccgcatagcc agcttgccca gagcagcgag agcgttgcac gttgcgaaaa gagcaagacg
     7861 ccgagtgata acaggaagac ggccgcttca taagcggcaa agaaatttgg attgaaaaat
     7921 gtgccatgcg ctcgaggttc tcctaaccag agatattgac ttattcccca gatcccctcc
     7981 gacacaccaa ggccactcac caagaggacc agtgcccact tctggccagg cagtcggatc
     8041 ccctgcgtga ccatgacgaa gagcatggca taggacacaa tgttgaggat ccactgcacg
     8101 ctggcattct tgtaaggcga ccatgtgatg gacaggatcg accaaccaca gaacagcgcg
     8161 accgagaccg ccagaggggt ttgagggagg aagagagaac cctctcgtat tcgacccaca
     8221 agccatatcc ccgcacttac cagaatcacc aggcggatga ggagaacggg cagttgcgtc
     8281 gtgcccccgt cgatcaaagg agagaacagc accagccacc cgatggtcat gaatagggcg
     8341 ataccaggcc aggcagcaat ctcgcgcttc aaccatgctg tggtgtcact gatatgggag
     8401 acgaaggcac cggcttgcac ccgtccccca acggacaatg cgtccggaag agtgttgatg
     8461 tggccaggat ggtcgggcac cgaactcctc ccctccgaac gacaaaaaga cacgattcga
     8521 gatggcatct agagacgcat agaggccagt gatttaaccg tatgaggggt gcgagggagt
     8581 gtcaagaatt tctcgtcttc atacgttaaa aacaacaagg gagggagacc tcatgatctc
     8641 cctccctccg gatccgcaga tccgttcaca ctgtgacagc gacggtctta caaggacacg
     8701 tcgttgtttg tgttctgcag attgcgctgg tcattgatga accagtgatc gcaagccgta
     8761 tcaccatcga tgttgccgtt cgcaccggca gtgaatccac ttgatgtcgc cagcacggag
     8821 aaaccaccgc tggtcggcgc cacggtcacg ttacaggggg tcgctgcgtt cgaactgcca
     8881 ttgaagcggg tcaccgtcgt accggttcca gctgcgttca caccgtcctg ataccagaac
     8941 gagtatcggg gcgagccgga gggctgccac ccgatctggc tgatggtcgc aggtgaatag
     9001 ctctgtgggt tctgcgattc agcttgatag gccacggcgg atgtgaagat ggctcccaaa
     9061 ctgaccttcg cttccgactg cttggacttc gcctgatagg cgacgaagtt cggaatggcg
     9121 atggccgcca agataccgat gatcgcgacc acgatcatca actcgatcag agtgaaaccc
     9181 ttttgacctt tgaacgactt acacattgct gctcctcctt gttgatggtt cgcgcacgct
     9241 ccgaagagcc ttattgccaa atccgtcctg aactcggcac cctctgcctc accccgcagc
     9301 agtagcaggt tcggtgccga aggcggcctc aggcgatcag ccaggggacg agcacctttt
     9361 caatgactta tggggatgtg caggctgagg agcgccaaat gtcgctaccg agttatgctc
     9421 agatccgcca attattgtca ctgcgcgcag gagcagtaga aatcgcaggg gcaattgttt
     9481 agcaagtggt tctactcagt tgcagagagg ccggattccg cagtcagcac ggactgcgtt
     9541 cctgtcgttc ctgtcggcat tccattggcg cctattggtg gaaaagacgg aaacaatttc
     9601 gatacagggc caaccgattt ggatacgcct tccttcttca ccttcacaca gaagagcagg
     9661 gttaccagga cgaagccaag cccgctgagc catgccccaa ctttcaacaa cggcgtctca
     9721 tactgaaaca cgatgcggtt gtggcctccc gggacaagca cggcacggac cagcccgttc
     9781 gcccggaaaa tttcaagctg gtctccgcct acaatgttgg ctttccatcc cggttcaaag
     9841 gcgtcagtca acaccaagac cgctgggcct tgcgtctgca cgtcgacttg aacgcgttcg
     9901 ggctgatact cggctatgga cgcatggccc accggtgagg cgcccggcaa cggaacgggt
     9961 gcgccttcga tcccgtccac ctgcgcgctc aagaactcct gtcgttgcag cagatcgcgc
    10021 atcggcatcg ccggtgccat cacttccggt cggcgagaca agtatgcgcg aggagtcacg
    10081 ggcaccgggt ttcgcacgag agccaggtcg tagtccgcaa tgctcgcgac aacagacctc
    10141 gcaaatgaat cgctctcgaa tcgtgccgga cgacctataa gataggccac gttgtagcgg
    10201 gccgccacct gtagactcgc gttcctgcca atctcgtcga ctcgagggct gagtccggcg
    10261 aggtaatgtt ggatagattc gattcgaaag accgcgttgt gttcaaggta cagaccattg
    10321 cggcgaagag cggcaatacg ctcacgggac gtcagccgtt tttccacctc ttcggatact
    10381 gccgccatac tgtcctttat gctcagtata cggaaatggc ctggaccctg tgccttggcg
    10441 tcctctgaaa gagccgacac gagacccgga gtaaacgtcc aggcctctga ggaagacgtt
    10501 tgcaccatgg gcaggttaac cctcgcgaga tccagaacca tcaacaacac aagggccgcg
    10561 ccgcttgcaa actggtcggc gggccggatc gccatccatc gaagaatcat ccccatgccc
    10621 gctgccgctg ccccggcgta caaagctgag acggccatgg catgggagat atgcgacgcc
    10681 aaatccgtgg ggagtaagaa taacttcgag aaaaagtgcg gtccctgttg cgtccccagc
    10741 actccggcaa gcgcggccag caggccggcc gtcagatacc acaagcaggt tcccggacga
    10801 ccccgacgca ggacgtccac gccgcccgcc gcaagcaacg cgaggccaaa cgtcgcaatg
    10861 cccatcaatt tttccggata gcgaaacgcc gaccacaacg gcacccagtc atagaacagg
    10921 tcgtagacgc ccgcgtaaga gcccatcgcg agggtaatgc tggacacagt aatcactaca
    10981 acaacgagca tcccactgcg tcgcaatcct gccagcccaa ggcctattac gggaagtcct
    11041 aggtacaaac tttccgccca aaagcccgcc ggaccgcgcc cctgctcttg ggtgtgaaac
    11101 aacgtctctg cgattcgatc gccttgcgcg ctgtctccga tcggcgacat gagcattgtc
    11161 agcagtcgta gggggtgggt tgaccagtgt atggcttcgg catgaaacaa cgcaggatcg
    11221 gtcctgtcgc tgcgctgata gctgatccat gacggcgcaa gctggacggc cgccaccaga
    11281 acggtaaggg cggcgacggc aatcagacgg ccgaataatc ctgcctgttt cccgcgcatg
    11341 atcgtccata tgacggccac gccaccatag taataggccg tctggatgtc tccatgcaga
    11401 atgacgctcg cccagatcag tgccgatgca ccaatccaac gcaacgtgcg agtcaccatc
    11461 agcttttcaa tcgagcaaac gaagagaggc aaggtgcaaa gcgagtacag atatagaata
    11521 ttctccgtca gggacatcac ataacctgaa caggaaaaac caactgaccc gatcaccgca
    11581 ccgactcgcg atatgcccag gacacgggct aatagaaaag ttccggaggc agccatgaga
    11641 caagaaagca acgtcgaaag gcgataggca tcctgcacgg gcaacagcca atacagcgcc
    11701 gtgaacggat ggaacacgcc catcaccgtc agcgcgataa gcggacgccc aaggccttcg
    11761 taggggaacc actcaggcag ttcgcctgca gaaagacgat caatgatgta ctgcttgagg
    11821 ggaagaaaga gttgaaaggc gtcccgcttg atcaacacga gtccggggag ccacagttgg
    11881 gcatagacga gcagcagcaa cccgatcagc cctgcgaggc agagcgcgtc gcgccgatca
    11941 agtcccgcat cgaagacccc gcccagtgcc ttcgacggct ccgtcggccc gagcgcacct
    12001 gcagggcgct ggccgcccga tatctcgtcg ggctccgtaa tggcatggtg gtgcaccaca
    12061 acggcaggag tctagcagga ttgaagatga tcgcaagaaa atgactggtt cgctcgttat
    12121 tgcgcgaccg
//
LOCUS       DASTQY010000492         4961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_693525, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000492 DASTQY010000000
VERSION     DASTQY010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693525"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..827)
                     /locus_tag="VFR82_15755"
     CDS             complement(279..827)
                     /locus_tag="VFR82_15755"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU4503105.1"
                     /translation="MQTRTVLTICTGLAIGVALITGPAFGRAEEKNMSDSWITAKTKI
                     ALFADSRVKGSDVSVATTNGMVIIRGKVDTETAKQAAEAIAKDIQGAKAVKNDLQVVA
                     PSKREAIDDKDDTITARVNEQMAKDAQLKTAGINAQTNAGVVSLSGEVADLSTSAQAS
                     WSAWQVPGVKAVKNDLMVKEKA"
     gene            complement(891..1106)
                     /locus_tag="VFR82_15760"
     CDS             complement(891..1106)
                     /locus_tag="VFR82_15760"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU4503106.1"
                     /translation="MNADQLRGQWMQFKGDLKQKWGNFTDNDLEEIGGSYDKFVGKVQ
                     ERYGDKKSELMKWTEAWQSQSASGDTK"
     gene            complement(1266..1406)
                     /locus_tag="VFR82_15765"
     CDS             complement(1266..1406)
                     /locus_tag="VFR82_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503107.1"
                     /translation="MTPILHTPMVILGWYEAPLTVYLLRFLTFPSVTDSSVSAAPMPE
                     IL"
     gene            1772..2077
                     /locus_tag="VFR82_15770"
     CDS             1772..2077
                     /locus_tag="VFR82_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503108.1"
                     /translation="MNADMYKSLQRSWGLASKRRNVDKKGLAVALTFLVVMLFGMREC
                     SDRTIGQVTTNALQQPDLSTEIEIRSAGRSPREAEFSGPGQSMTGVRRELDDPQGSR"
     gene            complement(3134..4198)
                     /locus_tag="VFR82_15775"
     CDS             complement(3134..4198)
                     /locus_tag="VFR82_15775"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEU4503109.1"
                     /translation="MLRSTRAVSQSLFIPADLRPGRMLLKRIGILLALVALVLLMLWV
                     ERDGLKDHHHDGEMSFTDVLYFAMVTITTVGYGDIVPITPRARLLDALVVTPVRVIIW
                     LLFLGTAYQLVVRQYMEGYRMGKVQAALNDHTLVCGFGYTGWSAAKELLAKGVAAERI
                     VVIDPNEDRIRAALELGLVAFRADATQETTLKHAMIQCARAVIISAGRDDSNALILLT
                     ARHLNPTVEILVSAVEEENVKLFRQGGATGIISPSTFGGYLLAAAVGHPHFTEYVQDL
                     LTAGGPVTLIERQPKPEEIGKSAAALKPDILLRLYRRGEVYNFWDLQESQRMEAGDTL
                     LILTSAGRTSASRQDHSKAA"
     gene            4491..>4961
                     /locus_tag="VFR82_15780"
     CDS             4491..>4961
                     /locus_tag="VFR82_15780"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU4503110.1"
                     /translation="MKHHDQTSRPSAEPFSPPGMTRRGFVTATLAAGFAFAVRPVSAA
                     TITTDETGLTTGEIRIRTADGHIPAYRAKPKKGSAFPVVLVVQEIFGVHEHIKDICRR
                     LAKLGYVAIAPELYARQGDVLKMTDIDEIRKVVSKVPDAQVLADLDATVAWIKES"
BASE COUNT         1038 a         1443 c         1393 g         1087 t
ORIGIN      
        1 acttttgatc agacgccgtc tcaccgcaag cacagaattg agttgaggcg caaacaccca
       61 tctgagtctg aaaggcatct catggcacat ccagtcgtcc aatcgcgtga ggcgctgacc
      121 gcagctagac atatctggac ccggcactgt ccgacgtgcg gaaagtggct gaaaaaaacc
      181 ctaccacttg atccctggat ctgcgcctgc ggatggcgat gtccgtgagc agggtccgcc
      241 tccgccgctg aaacaaacgg cggagaagaa ctcctacctt acgccttttc tttcaccatc
      301 aaatcgttct tcacggcttt cacccccgga acctgccagg cggaccaaga ggcttgggca
      361 ctggttgaga ggtcggccac ttcgccgctc aatgagacca cacctgcatt cgtctgggca
      421 ttaatgccgg cggttttcag ttgcgcgtct ttggccatct gttcgttcac gcgagccgtg
      481 atcgtgtcgt ccttgtcgtc gatcgcttcg cgcttggacg gagcgaccac ttgcaaatcg
      541 ttcttgaccg ccttcgcccc ctggatgtct ttggctatgg cttccgcggc ctgcttcgcc
      601 gtctcggtat ccaccttgcc gcggataatc accatgccgt tggtggtcgc gacactgacg
      661 tcgctgccct tgactcggga atcggcaaac agcgcaattt tggtcttggc tgtaatccac
      721 gagtcgctca tgttcttctc ctcggccctg ccgaacgccg gtccggtgat gagcgccaca
      781 ccgattgcca aacccgtgca aattgtcaga acagttcgcg tctgcatatc ttctccttac
      841 ccgtaacagt atgtcgtcaa tcgtatggtg cattgcggtt tgcagccgga ctacttcgtg
      901 tctcctgatg ccgactgtga ctgccaagcc tccgtccact tcatcagttc actctttttg
      961 tcgccatacc gttcctggac cttgccgaca aacttgtcat agcttccgcc gatctcttcc
     1021 aaatcattat cggtaaagtt tccccacttt tgtttcaggt cgcccttaaa ctgcatccat
     1081 tgtcccctga gttgatcagc attcatcgca tcgccctcct tgtatgtaca gcagtcacgt
     1141 tgatgatcac aggtcccgta ccgtcgtaca gcacacctgt ccggtaaaat ctgggatggt
     1201 catctgaggc aggccggcgt cgcgtccgct cctgcagcgg accatcacac acggcatgcg
     1261 gccgtttaga gaatttcggg catcggagcg gcggagactg aggaatcggt cacgctggga
     1321 aaggtcagga agcgtaagag atagacagtc agcggagctt cataccagcc aagaatcacc
     1381 atgggcgtat ggaggatagg agtcaagcac tataatccat ggctggatga tcaacggtcc
     1441 tcttcgactc tgacatgctg cggatccatc atgcgacgca taccatttga ttggcatgta
     1501 tagacattct tgcgatcatc aaattgtgtc atgcacgacc atggccatta ttagtgcttg
     1561 actcgagggc gtttttcgct tagcgtgtgc aaaggatgta ctgaatctcg acttccgacc
     1621 ccgtcgcgct tcgtcttccc gcctacgttg tctccgccgt ttctacgcca acgtttcctt
     1681 taagacgcgt gtaaggtgtg atggtgactg ggagcggtgt cccggcagct cgattgtacc
     1741 gggtagggat agaagattct gaaggaccaa catgaacgca gacatgtata aatcgctaca
     1801 gcgtagttgg gggttagcga gcaagagacg gaatgtagat aagaaaggcc tggcagtcgc
     1861 cctgacattt ctcgtcgtca tgctcttcgg gatgcgagaa tgcagcgaca ggacgatcgg
     1921 gcaggtcacc accaacgcct tgcagcagcc ggatctctcc actgaaatcg agattcgttc
     1981 ggcgggcagg agccctcgcg aagcggagtt ttcagggccg ggccaatcga tgacaggggt
     2041 caggcgagaa ctggacgacc cgcagggatc tcggtgacgc catgaggtct gtctggaggg
     2101 aacgtccatt accacatacc tcgttcaacg ggagaaaggt atctttgaaa atcaagaaag
     2161 aggtgcgaga attcatcatt gcgtcggaag ccctatatag gatcttttcc cacggggtac
     2221 cgctcagctg ccacgaagcc gaaattctaa actgttgcat ggacgagctc aaccgacggc
     2281 ggcttgctcc tcgtctcgca attcactgaa ccagagcgtg cccaattccc aaacattgtt
     2341 tctgaaccga ggaggtcttg atgtcgtcgt ttactgtaac cattatgggt actactgccg
     2401 aaaccgaaag tacgttcctg atgcatgttg tccaagcggc tctgaaggcc tatcgtcacc
     2461 atcgcaatac taacgaccaa gtgaatggca acgggttggg ccatgcaacg aacgacgccg
     2521 aacaagcatg gactccatca gcacatcttg taagcaggta gcgatcctat gctccccgaa
     2581 agtctgagac cggctctcct ttgtttcccc cttcccctgc tctccggtga tctccccgcc
     2641 ctaatcggcg gctacaatct cgcgagccgg cgcgttgtac tcgcctgggg catccgatag
     2701 tctcccagat gactcgccgg acgtagaata ggttcactca aggtgggcga aacacggggg
     2761 tcatcagatg ccggcgtatg agtacaagtg tttggattgt ggcaaggagt cggtgctgaa
     2821 tctaagcctg agcgagcacg agctcggcaa gattacatgc ccgggatgca agagcgcccg
     2881 catggagcaa ttgatctcgg cgatctcggc aaagacgagc cgaaaaagtt aattgctgac
     2941 tcctccattg ccttttcatc cgtgactccg aggatgcctt ccgagagtgc ggtcgcgtgt
     3001 cctcaaagag cggaccgacg tgtgtgcaat caggcagctg gtttgtttcg accttggaat
     3061 tgttgactgc tcaccgtgtg gaccggccgc ccacccgtgt catggtttga acgaccgtcc
     3121 cttccctacc atcctaggcc gccttggaat ggtcctggcg agatgcggaa gtgcggccgg
     3181 ccgaggtcaa gatgagaagg gtgtcgcccg cttccattct ctggctttct tgtaagtccc
     3241 agaagttata gacctctccc cggcgataca ggcgcagcag aatatccggc ttcaatgccg
     3301 cagcgctctt tccaatctcc tccggcttgg gctgccgttc aatgagggta accggtccac
     3361 ctgcggtgag cagatcctgc acgtattcag tgaaatgggg atggcccacg gcggcggcga
     3421 gaaggtaccc gccgaaggtt gacggcgaaa taatgccggt cgcgccgccc tgtcggaaca
     3481 gtttgacgtt ctcctcctcc accgcgctga caagaatttc caccgtcgga ttcagatgcc
     3541 gggcggtcaa gaggatcagc gcattgctgt cgtcgcggcc ggccgagatg atgacggccc
     3601 gggcacactg gatcatggca tgtttcagcg tggtctcctg cgtggcatcg gcccggaagg
     3661 cgaccaggcc tagctcgagc gcggcgcgga tgcgatcttc gttcggatcg atgacgacga
     3721 tgcgctccgc cgcgactcct ttggccaaca gttccttagc cgccgaccag ccggtgtagc
     3781 caaatccgca gacaagcgtg tgatcgttga gcgcagcttg cacttttccc atacgatagc
     3841 cctccatgta ctggcggacc acaagttgat aggccgtacc gaggaacagc agccagatga
     3901 taacccgcac cggcgttacg accagcgcgt cgagcaggcg cgcgcgtggc gtgatgggaa
     3961 caatatcgcc gtagccgaca gtcgtgattg tgaccatggc gaagtacaag acgtcggtga
     4021 aggacatctc cccatcgtga tgatgatcct tcagcccgtc ccgctccacc cagagcatca
     4081 gcagcacgag tgcgactaac gccaacagga tgccaatgcg cttgagcagc atccgcccgg
     4141 gccgcaggtc cgcggggatg aaaagagatt ggctgacagc gcgggtcgag cggagcatca
     4201 cacaccaagc tatccagaac agatactact tactcgagac taccggtttc tgggcagccc
     4261 ccctcctagg atgtttccga gtcccgtgtg ttggggagca tggtcatctg ggtttattgt
     4321 ctggcccacg cgtgcgtgct tgtggccatg gtattctcgt gggagggcct ctggcacagc
     4381 ggcatccacc ggctgtgact gcatcgggcg ggaaggaaga gggcaggacg gcgcacggtc
     4441 tcttgtgata atccagacat cagtccatct ttttagcggg gtagaatgcc atgaaacatc
     4501 acgaccagac gtccagaccg tcagccgaac ccttctcccc tcccggcatg acgcgccgtg
     4561 gattcgtcac cgcgacgctg gcggccggct ttgcattcgc tgtgcggccg gtctccgccg
     4621 caaccatcac gacggacgag acagggctga cgaccggcga gatccggatt cggaccgctg
     4681 atggccacat ccccgcctat cgagcaaagc cgaagaaggg cagcgcattc ccggttgtgt
     4741 tggtggttca ggagatcttt ggcgtgcacg agcacatcaa ggacatttgc cgccggctgg
     4801 ccaaattggg ctacgtggcc atcgcgcccg aactctacgc tcgtcagggt gatgtcttga
     4861 agatgaccga catcgatgag atccgcaaag tcgtctcgaa agtccccgat gcgcaggtct
     4921 tggccgatct cgatgccaca gtggcctgga tcaaggagtc g
//
LOCUS       DASTQY010000493         3648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_926280, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000493 DASTQY010000000
VERSION     DASTQY010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926280"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..815)
                     /locus_tag="VFR82_15785"
     CDS             complement(45..815)
                     /locus_tag="VFR82_15785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4503111.1"
                     /translation="MITRWLAASLFFVGLIAVWHLVVKAQIWSPLLLPSPVSVLDYLR
                     SAAEDGTLWEATLVTVRRLLIGYGLGILIGLPLGLLTASSRWCHDTIGILALGLQTLP
                     SVCWVPLALLWFGQSEAAMLFVVVMGTLWSLIIATDNGVRTIPPIYRRAARTMGSTGL
                     HTWTHVILPAALPFLISGMKQGWAFAWRSLMAAEIYVTILSGFGLGQLLHYGRELHAL
                     DQVIGVFIVIVLIGLAVDKSLFAPTERFMYRRWGTERA"
     gene            complement(812..1618)
                     /locus_tag="VFR82_15790"
     CDS             complement(812..1618)
                     /locus_tag="VFR82_15790"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4503112.1"
                     /translation="MFTTEHPQVRPQASKLVLERVSKWFQTSSLHVHALDDVSLRIAE
                     GEFVCLVGPSGCGKSTLLNIIAGLEHPDRGTVQADGLAVDEPGPHRLMMFQESALFPW
                     LTVLGNVLFGLKLRNGMSAAERREKAEYFLELVGLKRFMHSNVHELSGGMKQRVALAR
                     ALAPNPSVLLMDEPFGALDALTREQLYGDIQRIWSQHRKTIVFVTHNVREAVCLGDRV
                     ILFSPHPGRIREEFAIPLPRPRDINSVDLARFATEIMGVLKGYVQSEVAG"
     gene            complement(1620..2588)
                     /locus_tag="VFR82_15795"
     CDS             complement(1620..2588)
                     /locus_tag="VFR82_15795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4503113.1"
                     /translation="MHMMMWTVVFGSFMATMPSIVNAGDTIRVGHFPNVTHVQGLVAH
                     HFSRKGQGWFEQRLGPDVKIEWYIYNAGPSAMEAILADSIDLTYVGPSPALNAYTKSN
                     GEEIRIVAAAAAGGAALVVQADSLLKQPSDFRGKTIATPQLGNTQDIAARAWLGNGGL
                     KITQTGGDASIIPTPNPDQLALFKNRKLDAVWTVEPWVSRLERDAGGKVLVEQSHESI
                     TVLVSSVKFLKTKRDLAAKFIRAHRELTEWILSHPEEAKQMVRQELAAETKAEVSSEL
                     IAQSWKRIVLKNDASLDEYQHFVANAQRAGFIRSLPDLSRLIEKVN"
     gene            complement(2598..3362)
                     /locus_tag="VFR82_15800"
     CDS             complement(2598..3362)
                     /locus_tag="VFR82_15800"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEU4503114.1"
                     /translation="MDVFKSTRPHIGIVAGTADGASLCYRSLCYEAVRTGRPAQPEIT
                     MHTFPLEWYLSLIERDDWAGVAALMSRSAGKLVQAGAELIICPNNTLHRAFDLVVSAV
                     PWLHIVEPVTAEIRRMRLQRVGLLGTPAVMEGRIYHPQLTQAGIEVVLPEAQERSRLH
                     HIIRTELIHGQYTATSRSCVQEIIGRLAARGTEAVILGCTELPLLLADEQSALPLLDS
                     TRLLAKAALQRCAPAATSRSALAKGVSAPPHVSISQ"
     gene            complement(3413..>3648)
                     /locus_tag="VFR82_15805"
     CDS             complement(3413..>3648)
                     /locus_tag="VFR82_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503115.1"
                     /translation="EPDISKYDRQMPNVRERSAEFDVTFEPEDYLIKVKSAGKLFAPD
                     QREELQIKYREALAALPTYPGLEPRQPSQPSQP"
BASE COUNT          796 a         1044 c         1047 g          761 t
ORIGIN      
        1 catggcgcta cggtaccata ggagccatgc gggtgccagt gaggttacgc gcgctcggtc
       61 ccccatcgtc ggtacatgaa tcgttcggtg ggggcgaaca gggacttgtc gactgcgagg
      121 ccgatcagaa cgatgacgat aaagacgccg atgacctggt ccaaggcatg cagttcccgg
      181 ccataatgga gaagttgacc gagaccgaag ccgctcagaa tggtcacgta gatttctgcc
      241 gccatcagtg aacgccaggc aaacgcccag ccttgtttca taccgctgat caaaaatgga
      301 agtgctgccg gaaggatcac gtgcgtccac gtgtgcaggc cggtagagcc catggtgcgg
      361 gcggcgcggc ggtaaatcgg gggaatggtc cgtacaccgt tgtctgtcgc gatgattaat
      421 gaccagagcg tgcccatcac cacgacaaac agcatggcgg cctcgctttg cccaaaccag
      481 agcagggcca gcgggaccca acatacactg gggagcgttt ggagcccaag cgcgagaata
      541 ccgatcgtgt catggcacca tcgcgatgag gcggtcagca gcccgagggg taatcctatg
      601 agaatgccca agccatagcc gatgagcagc cgtcgtacgg tcaccaacgt ggcttcccag
      661 agtgtgccgt cctctgcagc acttcgcaga tagtcgagaa cgctcacagg ggaaggcagt
      721 aacagcggtg accagatttg agccttgaca accagatgcc agacagcgat caagcccaca
      781 aaaaacaacg atgccgccaa ccatcgtgtg atcaaccggc cacctccgac tggacgtagc
      841 ctttcaagac tcccataatt tccgtcgcga accgcgccag atccacactg ttgatgtctc
      901 ggggcctggg aagcgggatg gcaaattcct cacgaatgcg cccggggtgt ggagagaaga
      961 ggatcacgcg gtctccgaga cagaccgctt cgcggacatt atgggtgacg aacacgatgg
     1021 tttttcggtg ttgactccag atccgttgaa tgtcgccata gagttgttcc cgtgtcagcg
     1081 catccaatgc accgaacggt tcgtccatca gcaggacgct cggatttggt gcgagggccc
     1141 gtgccaaggc cacgcgttgc ttcatgccgc ccgagagctc atgaacgttc gaatgcatga
     1201 agcgcttcag tccaacgagt tctaggaaat actccgcttt ctcccggcgc tcggctgcgc
     1261 tcatgccgtt acgaagcttt aatccaaaca ggacgttgcc cagcactgtc agccatggaa
     1321 atagggccga ttcttgaaac atcattagac gatgaggtcc aggttcgtcg acggccaggc
     1381 cgtcggcctg gacagtccca cgatcaggat gttccaagcc ggcaatgata ttgagcaagg
     1441 tcgactttcc acatccgctg gggccgacga ggcagacaaa ttcgccttct gcaatccgca
     1501 gcgacacgtc atcgagggca tgcacatgca gcgagctggt ctgaaaccac ttcgagacac
     1561 gttcaaggac gagcttcgat gcctgggggc gtacctgggg atgctcggtc gtgaacatgt
     1621 caattgacct tttcgatgag ccgcgataaa tcggggagcg accggataaa gcccgcccgc
     1681 tgagcattag ccacgaaatg ttgatattcg tccagggacg cgtcgttttt gagcacgatg
     1741 cgtttccatg actgtgcaat caactctgat gacacctcgg ctttggtttc ggcggcgagc
     1801 tcctgtcgca ccatctgttt ggcttcttcg ggatgggaga gaatccattc cgttaattcg
     1861 cggtgcgcac gaatgaactt ggcagccaga tcacgtttcg tcttcaagaa cttcacactc
     1921 gaaaccagga ccgtaatgct ctcatgtgat tgttcgacga gcaccttgcc tcctgcgtct
     1981 cgttccaaac gcgaaaccca aggttcgacc gtccagaccg cgtcaagctt tctgttcttg
     2041 aataacgcga gttgatccgg gttcggcgtc ggaatgatgg aggcgtcgcc gcccgtctgg
     2101 gtgatcttca aacctccgtt gccgagccag gctcgagcgg caatgtcctg agtattgccg
     2161 agctgcggag tcgcgatggt ctttccacga aaatcggacg gctgtttcag caacgagtct
     2221 gcttgtacaa ccagcgccgc acctccggcg gcagctgccg ccactattct aatctcttca
     2281 ccattggatt ttgtataggc attcaaggcc gggctcggtc cgacataagt gaggtcgatc
     2341 gaatccgcca agatggcttc catggcgctt gggccggcat tgtagatgta ccattcaatc
     2401 ttgacatcag gcccaagccg ctgctcaaac cacccttgtc ccttacgtga gaaatgatgc
     2461 gcgaccaggc cttggacatg cgtgacgttg gggaaatggc ccacccgaat ggtgtctccg
     2521 gcgttaacaa tggagggcat agtggccatg aaggacccga aaacgactgt ccacatcatc
     2581 atgtgcatgg gttcacctta ttgtgagatt gagacatgag gaggggcgga gactcccttc
     2641 gccaacgccg atcgggatgt cgcagccggt gcgcagcgct gcaaggccgc ttttgcgagc
     2701 aggcgcgttg aatccagcag gggaagagcc gactgctcgt cggcaagcaa cagtggaagt
     2761 tcggtgcaac cgaggatgac cgcttcggtc cctcttgctg ctagtcttcc gatgatctct
     2821 tgcacgcacg agcgagaagt tgctgtgtac tgcccatgaa tgagctcggt tctgatgatg
     2881 tggtgcaatc gactgcgctc ctgcgcctca ggcagaacga cctcaattcc cgcttgcgtg
     2941 agttgaggat gatagatgcg cccttccatg actgccggtg tgccgagcag tcccacacgt
     3001 tgcagtctca tccgccggat ctctgccgtt accggctcga caatgtgcag ccaggggact
     3061 gccgatacca ccaggtcgaa cgcgcgatgc aacgtattgt tcgggcaaat gatcagctca
     3121 gctcctgctt ggaccaattt ccccgccgat cgagacatca gcgctgccac gccggcccag
     3181 tcatcccgtt cgatgagact cagataccac tccagcggga acgtgtgcat cgtgatttca
     3241 ggctgggccg gccggcctgt acgcacagcc tcataacaga gactgcggta gcagagcgaa
     3301 gcaccgtcgg cggttcccgc gacgataccg atatgaggtc tggtcgattt gaatacgtcc
     3361 acgtggtatc tccttgagtc tgcacagcga caagcggcag gcccgcacac attcagggct
     3421 gtgagggctg actcggctga cgcggttcca accccggata ggtcggaagc gccgcaagcg
     3481 cttctcgata tttaatctgg agttcctcac gctggtccgg agcaaaaagt tttccagcgg
     3541 actttacctt gatcagataa tcttccggct cgaatgtcac gtcaaactca gccgatcgtt
     3601 cccggacgtt cggcatttgg cgatcatact tgctgatgtc cggttctg
//
LOCUS       DASTQY010000494         9238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_1366548, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000494 DASTQY010000000
VERSION     DASTQY010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9238
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366548"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1626
                     /locus_tag="VFR82_15810"
     CDS             223..1626
                     /locus_tag="VFR82_15810"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HEU4503116.1"
                     /translation="MPLRLRLSLWYGSALALILVAFSTVLYFVTARSLRDALDQSLEE
                     TAAAAVRSLEERGFLPLIDEDELMSQFPELARIDKFFQIFSPSGTITIRSPNVKQHEL
                     PLSRRALEVAFSGKTIFESAKYPKEPPLRLISVPIIYRGNLLYIVQVGTSMESVDQTL
                     NRLLLVLLVTMPLALAVSLAGGWFLAGRALRPVDAITLAAQRIAAGDLTQRLNVPASA
                     DEIGRLAGTFNNMIARLETSFRQIRQFSSDASHELRTPLTVMKGETELALRRPREAAD
                     YTVVLESNLEEIDRMTRIVDELLFLSRADMGEVKMERLPVKLESLLEDIHRQASLLGQ
                     ESNVQVILGLVLPAVVLGDELRLRELFLNLVDNAVKYSRPGGTVEISLTTHLNQAKLV
                     ITDQGIGISPEDQKRIFDRFYRTDDARAHTKKGTGLGLAICAWIVESHHGRIEVQSGP
                     NKGSTFTVTLPLAPPSS"
     gene            1739..3259
                     /locus_tag="VFR82_15815"
     CDS             1739..3259
                     /locus_tag="VFR82_15815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEU4503117.1"
                     /translation="MRDLVRRALGGLVGMALLGTMFFIGSQSFVPFPASGSPSATTTV
                     TGPVGTLPANGFTEVAKAVTPAVVNITTVIGEKISGRRGQDEMRDRMEEFFGGPNGPF
                     GPKGPSGPKFRGPEGPSEPRGFHGGGQGSGVIVAPDGYVLTNNHVIDGAREVTVTLPD
                     KREFPGRIVGTDPKTDLAVVKIEGKNLPTVAWGDASKLQVGEYVLAVGNPFGLNSTVT
                     LGIVSALGRGRMGITQYEDFIQTDAAINPGNSGGALVNTKGELVGINTAIFSQSGGYQ
                     GVGFAVPTSMGQPIYESLVKHGKVVRGYLGIGIQDLNQDLAKSFDLTDRKGALVTEVK
                     EDSPADQGGLKQGDVITSYQNLPVDDAVALQRLVTKTPVGSKVPLKVMRNGHEKDLSV
                     MVGEQPGDQKIAKADEEAKDFAFAGVAVQDLDRDAARELGLKGKTHGVVVTSVEPDSG
                     ADKAGLMPGDVIREINRQPVKSVKEFEKVSSTVKKGENVLILINRRGASLFLSAKV"
     gene            complement(3287..4789)
                     /gene="rimO"
                     /locus_tag="VFR82_15820"
     CDS             complement(3287..4789)
                     /gene="rimO"
                     /locus_tag="VFR82_15820"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEU4503118.1"
                     /translation="MNQALIQPERAKSPAPSSDSGKKTTVGFVNLGCTKNQVDSEIML
                     GTLAADGFELTGNPQKAEVVIINTCGFIEEAKQESINTILEHAKLKEFGVCRVLIAAG
                     CLAQRYQGDLLKELPELDGVVGTGEFGKIAGICRDLLTPKRRHQRLWISPPPYLYDEM
                     VPRLRLGKSHSAYVKIAEGCNRNCAFCAIPLMRGKQRSRPVASIMAEVQQLAEAGVME
                     INLISQDTVNYGVDLGLRQGLVCLLRELVKVKALRWIRPFYLYPQQVTDELLDLYAGE
                     ERITKYIDMPLQHINSAMLKRMHRLGDRRAIETLVERIRTRIPGVTFRTAFIVGFPGE
                     TDEAFGELKQYVEQADFDRVAAFLYSDEEGTPAAGLDQKIDRAVMEERRNELLGVQEA
                     IAAAKSMELIGSTLDVLVDGSAEEEGHWLEGRHEGLAPEIDGVVYIDGAGVESHRSGR
                     HGSGPSAGELVTVTITNATTYDVVGHLAGESSSSRELAASEPREPRGDYR"
     gene            4874..5413
                     /locus_tag="VFR82_15825"
     CDS             4874..5413
                     /locus_tag="VFR82_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEU4503119.1"
                     /translation="MIRTFQGIRPTIPKSCFIEDTGIVIGDVVMGEHCSIWFHAVVRG
                     DVHFIRLGNRTNVQDLCMLHVTHDTHPLIIGSDVTVGHSVILHGCTIKDRVLIGMGSI
                     IMDGAVIGEDSVVGAGALVTGGTVVPPKSLVLGAPAKVARPVTPKELSWIKESSDNYV
                     KYADQYMNGLPNSKTGFQP"
     gene            complement(5404..6135)
                     /locus_tag="VFR82_15830"
     CDS             complement(5404..6135)
                     /locus_tag="VFR82_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503120.1"
                     /translation="MTTGGRVSTVSRGAIAQVFDHLDYAALGKVYCYEGGGEFWKAKR
                     EPCRRLGSQIGTALLARLKPGGRSLYVGAGVAELPAMLIESLDLHRSVLPYNLRRREV
                     ASLNRACRALPLRFYAADAARATGPFDHLWIVSVLNDPERFPQLAALSYGRAMPVTFD
                     PRVFQQERNIVRVIVNRCLSKLALPGLVTTSTEEVVWIADWCHRHSIPYLVERKYYPT
                     ALVGDPVCFMRIGRPRERRQYRNQG"
     gene            complement(6132..6974)
                     /locus_tag="VFR82_15835"
     CDS             complement(6132..6974)
                     /locus_tag="VFR82_15835"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU4503121.1"
                     /translation="MKSLVGERHPHSAPEALARAAAYLAAQFAGFHWTTTRQSFRALG
                     KSYVNVLAIKAPVGRAVKERQAPLLIGAHYDTVMASPGADDNASALAVLLEVADQLKS
                     TVLTRPVWLAAFCLEEQGLLGSRAFTLHLKQTGQPLYGAIVLECVGYISHEAGSQGTP
                     PGIPITVPTVGNFLGIIGNDASRDLIAAVERSARSAAPAMPTLALAVPGRGEALPDVR
                     RSDHAAFWDEGYPAVMLTDTANFRNPHYHLPSDTLDTLDLGFIEAVSRTVAATVVELA
                     KAAR"
     gene            complement(7025..7921)
                     /gene="argB"
                     /locus_tag="VFR82_15840"
     CDS             complement(7025..7921)
                     /gene="argB"
                     /locus_tag="VFR82_15840"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEU4503122.1"
                     /translation="MNKLIRKANVLIEALPYIRTFRGKTIVIKYGGNAMTESSLKERF
                     AQDIVLLKYVGINPVIIHGGGPQIDEMLDRLGIESKFLHGVRVTNEATMEIVEMVLAG
                     KINMEITDLINRHGGSAVGLSGKDGGLILSKPLTARAWAESLERDLEEEDAESDFGFV
                     GDIEKVDPSLLFKLQGDHYIPVIAPIGTDREGNTYNINADLVAGAVAGALRAEKLVMM
                     TDIKGIRDAKSRHLSTVSRKDVQRMVKKGTITEGMLPKVHACLDALEQGVGKAHIIDG
                     RMPHAILLEVFTRKGIGTEITA"
     gene            complement(8016..8918)
                     /locus_tag="VFR82_15845"
                     /pseudo
     CDS             complement(8016..8918)
                     /locus_tag="VFR82_15845"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
     gene            complement(8936..>9238)
                     /locus_tag="VFR82_15850"
     CDS             complement(8936..>9238)
                     /locus_tag="VFR82_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4503123.1"
                     /translation="QLVGVEGYTDSAAMGGLAGINAVRELDRLPPLTPPPTTAHGCLL
                     SYITTSDPRHFQPMNMNFGLFPPLTVSVKDKERKKQLISQRALEDHAAWMSRSGLS"
BASE COUNT         1906 a         2716 c         2648 g         1968 t
ORIGIN      
        1 ttaatccata ccatccgggg cagtggctac atgctgaagg ccgactagca ggatgctgct
       61 gatctcaatt tttccttcac gcccatctcg gtccgtggtt ctggctcgcc cgtccctcga
      121 aacgttgaat cgtcgacacg cgaggcgtcg ttctccaggt ctcgtctgta cgccgacgca
      181 tgatcatctt ccgcatcaca tcgcctcttc gggagtgctt tgatgcccct acgcctccga
      241 ctgagcctct ggtatggcag cgccctggcg ttgattttgg ttgccttttc gaccgtcctc
      301 tatttcgtca cggcgcgcag ccttcgcgat gcgctcgatc aatccctgga agaaacggca
      361 gcggccgctg tgcgctcttt ggaagaacgg ggatttctcc ctctcatcga cgaagacgag
      421 ctcatgtcgc aatttcccga gttggcgcgc atcgacaagt ttttccagat cttcagcccc
      481 tcagggacga tcacgatccg ctctccaaac gtcaaacaac acgagttgcc gctgagccgg
      541 cgcgcattgg aggtcgcctt ttcgggaaaa acgatcttcg aatcggccaa atatccgaaa
      601 gagcctcccc ttcggctgat ctcggtgccg atcatctatc gaggaaacct gctctacatc
      661 gtgcaggtcg gcacatcaat ggagtcggtc gaccaaacat tgaatcgtct cctgctcgtg
      721 ctgttggtca ccatgccgct tgcgctggcc gtctcattgg ccggagggtg gtttcttgct
      781 gggcgggcgt tgcgccccgt cgatgctatt acgctggccg cacaacggat cgcagcgggc
      841 gatctcactc aacggttgaa cgtgccggcc tccgccgacg aaattggccg tcttgccggc
      901 accttcaaca atatgattgc gcggctcgag acctccttcc ggcagattcg ccagttcagc
      961 agcgacgcct ctcatgaact tcgtaccccg ctgacggtca tgaagggtga aaccgagctt
     1021 gccctgcgtc gaccccggga agcggccgac tacaccgtcg tgctggaaag caatctggaa
     1081 gaaattgacc gcatgactcg aatcgtcgat gaattgcttt tcttgtcgcg cgcggacatg
     1141 ggcgaagtga agatggagcg cctgccggtt aaactcgagt ccttgttgga agacattcac
     1201 cgacaagcgt ccctcctcgg gcaggaaagc aacgtacaag tcatactcgg cctcgtcctg
     1261 ccggcggttg tcttgggcga tgaattgcgg ttgcgtgagc tctttctcaa cctcgtggac
     1321 aatgctgtta aatactctcg gcctggcggt accgttgaga tatctctgac gacccatctt
     1381 aatcaggcga aactcgtcat tacggatcaa ggcattggga tttctcccga agatcaaaaa
     1441 cgaatctttg accgcttcta ccgcaccgat gatgcccgtg cgcacacgaa aaaagggacc
     1501 ggcctggggc ttgcgatttg cgcatggatc gtcgaatccc atcacgggcg catcgaggtg
     1561 cagagcgggc cgaacaaggg ctcgacgttt accgtgaccc tgccgctggc gcctccatcc
     1621 tcttaaagac ttttaatacc ctcctaatcg gtctctcatt accgcctgct accgtcttct
     1681 gtatagattt cggaccacct gacaacagtg tttcactttc aatctctaag gaggacctat
     1741 gagggatctg gttcgtagag cgctcggggg gctggtcggt atggccctgc tggggacgat
     1801 gttcttcatc ggcagtcagt catttgttcc ctttccggcg tcggggtcac cgtccgccac
     1861 aaccaccgtg accgggccgg tcgggacgct tccggccaat ggattcacgg aggtagcaaa
     1921 agcggtcact ccagccgtcg tcaacatcac gaccgtgatt ggagagaaga tctccggccg
     1981 acgcggtcaa gacgaaatgc gggatcgcat ggaggaattt ttcggaggcc cgaacggtcc
     2041 ctttggaccg aagggtccct ctggaccgaa attccgggga ccggagggcc ccagtgagcc
     2101 gcgtggattc catggcggcg ggcaaggttc cggtgtcatc gtagctccag acggctacgt
     2161 gttgaccaac aaccatgtga tcgacggcgc ccgcgaggtg accgtgaccc tccccgacaa
     2221 gcgggagttc ccaggcagga tcgtgggcac ggatcccaag accgatctgg ctgtcgtgaa
     2281 gattgaaggc aaaaacctgc cgaccgtggc ctggggggac gcgtcgaagc ttcaagtcgg
     2341 tgagtatgtc ctcgccgtcg gcaatccatt cgggctcaat tcaacggtga cgcttggcat
     2401 tgtcagtgcg ttaggccgtg gccgcatggg aatcactcaa tatgaagact tcattcaaac
     2461 ggatgccgcc atcaatcccg gcaattcggg tggagccctg gtgaatacca agggagagct
     2521 cgtgggcatc aatacagcga tcttttctca aagcggcggc taccagggcg tgggcttcgc
     2581 agtcccgacc agcatgggcc agccgatcta tgagagtctc gtgaaacacg gcaaggttgt
     2641 ccggggatac ctcggcatcg gcattcaaga cctcaaccag gatctggcta aatcctttga
     2701 cttgaccgac cggaagggcg cattggtgac cgaggtgaaa gaggatagcc ccgccgatca
     2761 gggcggcctc aaacaaggcg atgtcatcac gtcctaccag aacttgcccg tggatgacgc
     2821 tgtggcactg cagcgcctgg taaccaaaac cccagtcgga tcaaaagtgc cgttgaaggt
     2881 catgcgcaat ggccatgaga aagacctcag cgtcatggtc ggcgagcagc ctggtgacca
     2941 gaaaattgca aaggctgacg aggaggcaaa agactttgcc ttcgcgggtg tggccgttca
     3001 agacctcgat cgtgacgccg ccagggagct gggactcaaa ggcaagacac acggtgtcgt
     3061 ggtcacaagc gtcgaaccgg acagcggagc cgacaaggcc ggcctgatgc ccggggatgt
     3121 gatccgggaa atcaaccggc aaccggtgaa gtcggtgaag gagttcgaga aggtgtcttc
     3181 gacggtcaag aagggcgaaa acgtcttgat tctgatcaac cgtcggggcg cgtcgctgtt
     3241 cctcagcgcg aaagtgtaat cggcgagtca ccccgaggaa ctgtcgttag cgatagtctc
     3301 ctcggggctc acgcggctcg gacgccgcca gctcgcgaga cgaggaactc tctccggcga
     3361 gatgtcctac aacgtcatat gtcgtggcat tggtaatcgt gaccgttact aattctccgg
     3421 cactgggacc gctcccgtgt cggcctgagc gatgtgactc cacgccggca ccgtcgatat
     3481 agaccacacc gtcgatttcc ggcgccagcc cttcatgccg gccttccaac cagtgtccct
     3541 cttcttcggc agaaccatcc accagcacat cgagagtcga tccgatcagt tccatactct
     3601 tcgccgcggc gatggcttct tgcacgccta gcagctcgtt acgacgttcc tccatcacag
     3661 cacgatcgat cttctgatca aggccggctg ccggtgtccc ctcttcgtcc gaatagagaa
     3721 atgccgccac acgatcgaag tccgcctgct cgacatactg cttgagttct ccgaaggcct
     3781 catcggtttc gccgggaaat cccacaatga aggcggtgcg gaaggtgacg ccggggatac
     3841 gtgtcctgat gcgttccacc aacgtctcga tcgctcgccg gtcgcctagc cgatgcatcc
     3901 gcttgagcat ggcactattg atatgttgca gtggcatgtc gatgtacttc gtgatccgct
     3961 cttctccagc gtagagatcg agtaattcgt ccgtgacttg ctgcggatac aggtaaaacg
     4021 gtctgatcca gcgcagcgct ttcaccttca ccagctcgcg caacaaacac acgagcccct
     4081 gacgtaggcc gagatcgacc ccatagttta ccgtgtcctg cgagatcaga ttgatctcca
     4141 tgacgcctgc ttccgccagt tgctgcacct cggccatgat ggacgcgacc ggccggcttc
     4201 gctgtttgcc gcgcatcagc ggaatcgcgc agaaggcaca gttacggttg cacccttcgg
     4261 caatcttgac gtaggcgctg tgcgatttcc cgagacgaag gcgtggcacc atctcgtcgt
     4321 ataaataggg aggtggactg atccagagac gctgatgtcg ccgcttgggc gtcaagaggt
     4381 cgcggcagat gccggcgatc ttcccgaatt ctcctgtgcc gacgacgccg tcgagttccg
     4441 gcaactcttt gagcagatcg ccctgatagc gctgcgccag acaaccggcg gcaatcaaca
     4501 ctctgcagac accaaattct ttcagcttgg catgctccag gatcgtattg atcgattcct
     4561 gtttggcttc ttcgataaac ccgcaggtat tgatgatgac gacttcagcc ttttgcgggt
     4621 ttccggtcaa ttcaaaacca tctgcggcta gcgtcccgag catgatctcc gaatcgacct
     4681 gattcttcgt gcagcccaga ttgacgaagc ccactgtggt ttttttaccc gagtctgagg
     4741 acggtgcagg cgacttcgca cgttccggtt gaatgagcgc ttgattcata agttcatcag
     4801 tctacgagac gcctcaaaag actgtcaacg agccacttgc aggtgaacgg ctgattcccc
     4861 tatggtgcga acgatgatta ggacctttca aggcatcagg ccgaccattc cgaagtcctg
     4921 ctttattgaa gacaccggga tcgtcatcgg cgatgtggtg atgggtgagc actgtagtat
     4981 atggtttcat gcggtcgttc ggggtgatgt gcactttatt cgtctgggca accgtacgaa
     5041 cgtccaggac ctctgcatgt tgcatgtgac tcacgatacg catccgttga tcatcggcag
     5101 cgacgtcacc gttggccaca gcgtgatcct tcatggttgc acgatcaagg accgcgttct
     5161 catcggcatg ggatcaatta tcatggacgg ggcggtcatc ggtgaagact cggtggtcgg
     5221 tgctggtgcc ctcgtcacgg gaggcactgt cgtgccaccc aagagtcttg tgttgggggc
     5281 gccggcaaaa gtcgccagac cggtgacacc caaggagctg tcgtggatca aagaatcgtc
     5341 ggacaactat gtgaaatatg cagaccaata catgaacggt ttgccgaact ccaagaccgg
     5401 ttttcagccc tgattgcggt actgacgtct ttcgcgtggc ctgccgatcc tcataaaaca
     5461 gacaggatca ccaaccaggg ccgtaggata gtactttcgc tcgacgagat aaggaatgct
     5521 gtggcgatgg caccagtccg caatccagac gacctcctct gtggaggtcg tgacgaggcc
     5581 cggtagggca agcttcgaga ggcagcggtt cacgatcact cgcacgatgt tcctctcttg
     5641 ttgaaacaca cggggatcaa atgtcacggg cattgcccgg ccataagaca acgccgccaa
     5701 ctgcggaaac cgctctgggt cgttcaagac gctcacgatc cacagatggt cgaaggggcc
     5761 agtggcccgg gccgcatcgg ccgcataaaa ccgtagaggc agtgcccgac aggcgcggtt
     5821 cagcgaagcg acctcacgcc gacgaagatt atagggcaac accgatcggt gcaaatcgag
     5881 tgattcaatg agcatggctg gcaactctgc gactccggcc ccgacataca ggctccggcc
     5941 tccgggcttc aaccgcgcga gcagagcggt gccgatctgg ctgcctagac ggcggcaagg
     6001 ctcccgtttg gccttccaaa actccccgcc tccttcataa cagtacacct taccgagtgc
     6061 ggcatagtcg aggtgatcga agacttgggc tatcgcgccg cgggagaccg tactcacacg
     6121 gccccctgtg gtcatcgagc cgccttcgct agctcaacga cggttgcggc aaccgtacgg
     6181 ctcaccgctt cgatgaagcc gagatccaac gtgtcgagcg tatcgctggg cagatggtag
     6241 tgaggattgc ggaagttcgc cgtgtcggtg agcatgacgg ctggataccc ttcatcccag
     6301 aaggccgcgt gatcgctgcg gcgcacatcc ggtagggctt cgccacggcc gggaacagcc
     6361 aaggccagcg tcggcatggc cggagcggcg ctgcgggcac tccgttccac ggccgcaatc
     6421 aagtccctcg aggcgtcgtt cccaatgatg ccgagaaaat tccccaccgt cggtacggtg
     6481 atcggtatac caggaggcgt gccttgtgag cccgcctcat gactgatata gccgacgcat
     6541 tccagcacga tcgcgccgta aagtggctgc ccggtctgct tcaggtgcag cgtgaaggct
     6601 cggctaccca gcaacccctg ttcctcgaga caaaaagcgg caagccacac gggacgcgtg
     6661 aggaccgtgg atttgagttg atcggctacc tcgagtagga cggccaaagc actggcattg
     6721 tcatcggcgc ccggagaggc catgaccgta tcgtaatgcg caccgatgag cagcggagcc
     6781 tgccgctcct tgaccgctcg tccaaccgga gccttgatag cgagaacatt gacgtacgat
     6841 ttccccaagg cgcgaaagga ttgtcgggtt gtcgtccagt gaaacccggc aaactgtgcc
     6901 gccaagtatg ctgcggcacg ggccaacgct tcaggagcac tatgcgggtg gcgctctccg
     6961 accagcgatt tcatgtgacg gaggatactg gtctgccttg aggacacgtt ggacgggaga
     7021 cgagttaggc agtgatctca gtgccgatgc ccttccgcgt gaagacttcg agcaggatgg
     7081 catgcggcat gcggccgtca atgatgtgcg ctttcccgac gccttgctca agcgcatcca
     7141 gacaggcatg tactttcggc agcatgcctt cggtgatggt gcctttttta accatccgtt
     7201 gaacgtcctt acgcgagacg gtggacaaat ggcggctctt ggcatcgcgg atacccttga
     7261 tatccgtcat catcacgagt ttttccgcac gtaacgcacc cgcaaccgct ccggctacca
     7321 ggtccgcgtt gatgttgtac gtattccctt cgcggtccgt gccgattgga gcgatgaccg
     7381 gaatatagtg gtcaccttgc agcttgaaga ggaggctggg atcgactttt tcgatgtcgc
     7441 ccacaaaccc gaaatcactt tccgcgtcct cttcctcgag atcacgctca agactttccg
     7501 cccaggccct cgcggtcaac ggcttgctca atatcaatcc cccgtccttc ccgctgaggc
     7561 cgaccgcgct gccgccatga cgattgatga ggtccgtgat ctccatgttg atttttccgg
     7621 ccaacaccat ttcgacaatt tccatggtgg cttcgttcgt gacccgcacg ccgtgaagaa
     7681 atttcgactc gattccaagg cggtcgagca tctcgtcgat ctgcggcccc ccaccgtgga
     7741 tgatgaccgg attgatgccg acatatttca agaggacgat gtcctgggcg aatcgctcct
     7801 ttaaggagga ttccgtcatg gcattgccgc cgtacttgat cacgatggtc ttgccgcgaa
     7861 atgtgcggat atagggcaag gcttcgataa ggacgttggc tttccggatg agtttgttca
     7921 tcgtcgtctc ctcacgcgat gatcaatctg cagtcaggcg cgaccatatc accttcaggc
     7981 gttcaggccc agacaaattc cctcacaccg atctctcagg acgagccgcc ttttgaggtt
     8041 cggcctttgg acgtccgacc ggacctcgga tgagcgcggt cgtacacggc caacaactga
     8101 tccgtcgcga catgtgtgta gcgttgagtc gtactgagcg aagcgtgccc cagcatttcc
     8161 tggatcgcgc gtaaatctgc accttcgtca agcaggtgcg tcgcaaagga atggcgcagg
     8221 gtgtgtggac tcacagggcc gcctgacaag cggcttgaat aacgagcgac aattcgcgcg
     8281 acgctccggc tggtcaaccg gcctccacgc tggttacaga acagagggaa attgtcgcct
     8341 gctgcgtgtg gcggcctgtg cgatgtgacc agactgactt gatacagttt gattgcctcc
     8401 aacgcgagtt caccgatcgg cacaatccgc tctttccttc ccttcccccg aaggcgtacc
     8461 aggccttcgg actcatgaac atcgcccaca ctgagcgaga ccagctcgct cacgcgggct
     8521 cccgtcgaat agagcgtttc gagcatcgca cggtcacgca atgagccggc cgtctgtccc
     8581 gccggaaacg tcatcaaggc atcggcgtcg tctttggtta acacacgtgg aagagaccgt
     8641 ggtagtttgg gagtccgtaa ggcttcgacg ggattgtggc tcgtgagacc ccgacggctc
     8701 aggtagcgat agaagcttcg gatcgcggcc agttttctcg ccaatgacgc ctgcttttct
     8761 ccttttctgt cgagccattc gagatacgca cggacagcat ctgaggacaa ggaagccgga
     8821 tcgaccgggg atgaggccaa ccggctggac accagaaacc tgtctaattg ccgaagatcc
     8881 gaggcgtaat tccgaaccgt ttccggcgat gcccgctgct cgctctcgag aaacgttatg
     8941 aaagccctga tcgactcatc catgccgcat ggtcctcgag cgcgcgctgc gagatgagct
     9001 gtttctttcg ctctttatct ttgacggaca ccgtgagagg cggaaacagt ccgaaattca
     9061 tgttcatggg ctggaagtgg cgaggatcgc tcgtggtgat gtatgaaagc aggcagccat
     9121 gtgccgtcgt aggaggaggg gtgagcggcg gcaacctgtc gagttcacgg acagcgttga
     9181 tccccgcaag acctcccatc gcggctgaat cggtgtatcc ttccacaccc accagttg
//
LOCUS       DASTQY010000495         5686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_262907, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000495 DASTQY010000000
VERSION     DASTQY010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262907"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /gene="rseP"
                     /locus_tag="VFR82_15855"
     CDS             <1..570
                     /gene="rseP"
                     /locus_tag="VFR82_15855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU4503124.1"
                     /translation="VKDSPGKTLQVDVLRDGHRVSLSVTPLAESATVNGQAVQVGKIG
                     IAGPDRSLMRSANPLMAVYNGLEATWKWTELTIVGLYKMIVGDISSKNIGGPLTIAKI
                     SGEAAAQGPANVIFLIALLSINLGVLNLLPIPILDGGHLLFFLIEGILRKPLGERQRE
                     LAQQVGLVLLVGVMIFAFWNDLERIFFSH"
     gene            576..2279
                     /locus_tag="VFR82_15860"
     CDS             576..2279
                     /locus_tag="VFR82_15860"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU4503125.1"
                     /translation="MRTSQLLLPTLREDPGEAETISHRLMLRSGLIRKVAAGIYTYLP
                     LGLRIIRKVEHIVREEMNRAGAQELLMPIASPAELWRETGRWDFYGKELLRFKDRHER
                     DFCLGPTHEEVITDLFRREVRSYRQLPLNFYQIQTKFRDEIRPRFGLMRGREFIMKDA
                     YSFDKDEAGARTSYQKMYDAYHRIFSRCGLNFRAVEADTGLIGGSSSHEFMVLADTGE
                     ETVVYSESGTYAANVERAEALPPIQTSVEELRPLETVSTPGRRTVEEVTAFLKVDASR
                     LVKTLLYSTGTETVAVLIRGDHDANEIKIKRLLGVAQLELASPATVEKATGAPAGFAG
                     PVGLKLRIVADHAVKSATNVVVGGNRTDTHLVNANVNRDFQVEQFADLRNARAGDPSP
                     RKDGVLKATKGIEVGHVFMLGTKYSQTMNASFLDPSGKECLAVMGCYGIGIGRTAAAA
                     VEQHHDDKGIIWPYPIAPFHVHLIPLGQSGGVIEATTNLYQALETAGLEVLWDDRDER
                     AGVKFNDADLIGAPFQVIVGDKGLSEGLLEVKTRKTAQKTKLAPGEIPAKILQMASQA
                     S"
     gene            2484..4811
                     /locus_tag="VFR82_15865"
     CDS             2484..4811
                     /locus_tag="VFR82_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEU4503126.1"
                     /translation="MQTKPPTQNLQELQQKVEFAEHVKRITSQIHAASNLDQILLDLH
                     KDILSLFDAEDLTLFAFDADKKEIFYKVPHIDTIEEVRIPITEQSLAGFCAKYLRPVN
                     IADAYNVAELQGIHPSLNHDTSYDKRTGFKTRQVLTYPIVADNKYLMGVLQLLNKKSG
                     LRFTRKDEESVAEIAKALGIAFFNLKKVSTKKTSTKFDLLVTNGKITQNELDNAIAEA
                     RKSISSDLESILIEKYKVPKLEIGRSIAQFHKCPYIEYSERTLVDIELLRNLNVDYLK
                     KNHWMPLKRDRTAIEILTDDPGDLDRVADIKRTFPGLNIRFAVSLRRDISQFLNSATG
                     GSDTQAGRKLDENVSDILGELVTEAQAEAMEESGPGGGLDENDSAIVRLANQIIADAY
                     RQTASDIHIEPYGEKRETLVRFRVDGDCFEYMKIPQSYRRAIVSRLKIMASLDIAERR
                     KPQDGKIKFKLSETREIELRVATIPTAGYNEDVVMRILAASEPLPLDKMGFSDRNLKC
                     IKDIAEKPYGIILCVGPTGSGKTTTLHSVLGFINTPDIKIWTAEDPVEITQYGLRQVQ
                     VQPKIDFTFAKAMRAFLRADPDVIMVGEMRDKETAEIGIEASLTGHLVLSTLHTNSAV
                     ETVTRLLDMGCDSFSFADAMLGVLAQRLARRVCKDCKEQYAGTKEEYEEMRLGYGPDF
                     WDKLAIPMDNTFRLSRGKGCETCNRTGFKGRVALHELLLGTDKLKRLIQTRAKTEEMV
                     KAAIEDGMTTLMQDGIQKCLQGLTMFKEVKAVAIK"
     gene            complement(4936..5313)
                     /locus_tag="VFR82_15870"
     CDS             complement(4936..5313)
                     /locus_tag="VFR82_15870"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4503127.1"
                     /translation="MAELERQPQPSVELRRYPRHRVSAPFPCAFSLLGLKRWAAVDRN
                     GLGVVLDVSLGGAKVMSAAAMRPGDHLAVSFRMPDQASALQLDATVKWVNDQLFGLEF
                     LSVPLSVESRLKKFLSRSAYTCS"
     gene            complement(5320..>5686)
                     /locus_tag="VFR82_15875"
     CDS             complement(5320..>5686)
                     /locus_tag="VFR82_15875"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4503128.1"
                     /translation="RRPSALNRFRRHPRVRINTPFSCALSPVRSRGWLRKPIINVGLV
                     YDLSVRGARVSTEAPFKPGDQVALSLRLPKQIRSAEIAVATVRWTKDQFYGLAFRRLS
                     LDAYDRLKRFVAIASRAVA"
BASE COUNT         1379 a         1628 c         1575 g         1104 t
ORIGIN      
        1 gtcaaagaca gcccgggcaa gacgcttcag gtggatgtct tgcgcgacgg ccaccgggta
       61 tcgttgtcgg tgacgcctct cgctgaaagc gcgacggtca acggtcaggc ggttcaagtc
      121 gggaagatcg gcattgccgg gccggatcga tcgctcatgc gatcggcgaa tccgctaatg
      181 gcggtgtaca acggcctcga agccacatgg aagtggacgg aactcacgat cgtcgggctg
      241 tacaagatga tcgttggcga tatctcgagc aaaaacatcg gtggcccatt gaccatcgcg
      301 aaaatttccg gagaagccgc cgcccaaggt ccggccaatg taattttttt gatcgcgctc
      361 ctcagcatca acctgggtgt cctgaatctg ctgcctatcc ccattctcga tggcggtcac
      421 ttgcttttct tcctgatcga aggcatcctg cgcaaaccgt tgggagagcg acagcgtgag
      481 ctcgcgcaac aggtcggcct ggttttgctc gtgggcgtca tgatctttgc cttttggaac
      541 gacctcgaac gtatcttctt ctctcactga cattcatgcg gacttctcaa ctcctgcttc
      601 ccaccctgcg ggaagatccg ggtgaagccg aaaccatcag ccaccggctt atgcttcgct
      661 cgggattgat tcggaaggtc gccgccggca tctatacgta cctgccgctc ggtctgcgga
      721 tcattcggaa agtcgagcac atcgtccgcg aggaaatgaa tcgggcggga gcgcaggaac
      781 tgttgatgcc catcgcctct cccgccgagc tctggaggga aaccggccgg tgggattttt
      841 acggcaaaga gctgctgcgg ttcaaggacc gtcacgagcg ggatttttgt ctcggcccca
      901 ctcacgagga ggtgatcacc gatctcttca ggcgtgaggt ccggtcttac cgtcaattgc
      961 cgctgaactt ttatcagatt caaacgaaat ttcgcgatga aatcaggccg cgcttcgggc
     1021 tcatgcgagg ccgtgaattc attatgaagg acgcctatag cttcgataaa gacgaggctg
     1081 gcgccagaac cagctatcag aagatgtacg acgcctatca ccgcatcttt tcccgctgcg
     1141 gtctcaattt cagagccgta gaagccgata cagggttgat cggcgggagc tcatcacacg
     1201 aattcatggt cctggccgat accggagaag agacggtcgt ctacagcgag tctggaacgt
     1261 atgccgccaa tgtcgagcgc gccgaagctc ttcccccgat ccagacgagc gtggaagagc
     1321 ttcgcccact ggagacagtc agcacccctg gccgccgcac cgttgaggaa gtgacggcat
     1381 tcttgaaggt tgatgcctcc aggctcgtca agacgttgct ctattccacc ggaaccgaaa
     1441 ccgtcgccgt gctgattcga ggcgaccacg acgcgaatga gatcaagatc aaacgtctgc
     1501 tgggcgtcgc ccagttggag ctggccagtc ccgcgactgt tgaaaaggcc accggtgcac
     1561 cggcgggatt cgcaggtccg gtcgggctaa aattgcgcat tgtggctgat cacgccgtca
     1621 agtcggccac aaacgtcgtg gtcggaggca atcgcaccga tacgcacctg gtgaatgcca
     1681 acgtgaaccg cgattttcag gtggaacaat tcgcagattt gaggaatgcc cgggccggcg
     1741 atccctctcc gcggaaggac ggcgttctca aagccacgaa aggcatcgag gtgggccatg
     1801 tcttcatgtt ggggacaaag tacagccaga ccatgaatgc ctcctttctc gaccccagcg
     1861 gcaaagaatg cctcgcggtg atgggatgtt atggaatcgg aatcggtagg actgcagccg
     1921 ctgcggtgga acaacatcac gacgacaagg gcatcatctg gccctacccg atcgctccct
     1981 ttcatgtcca cctcattcca ttaggccaat caggcggcgt gatcgaagcc accaccaatc
     2041 tttaccaggc gctggaaacg gcgggtctcg aagtgttgtg ggacgatcga gacgaacgcg
     2101 ccggcgtcaa attcaacgac gccgacttga tcggtgcccc ttttcaagtc atcgtcggcg
     2161 acaagggtct gagtgaaggc ctcttagagg ttaaaaccag aaaaaccgct caaaagacca
     2221 aacttgcccc cggggaaatc cccgcgaaaa ttctgcagat ggcctctcaa gcctcttgaa
     2281 caaccaccgc tacgatgagc gttgtccgca tcccctaaag tcgttcgcct tgggaatcgt
     2341 gaaagcgcct tttccttgcc ttcccaccct gttgggctag actgaatctc gaatcatcac
     2401 agtgaggcaa ttgagccacg gtggccaaca gccgccggca cgaccgcctc cgtgatccgc
     2461 tttatcgtca caggagctta gtgatgcaga ccaagccgcc gacacagaac ctgcaggaac
     2521 tgcagcaaaa agtcgaattc gcggagcatg tcaaacggat cacgagccag attcacgccg
     2581 ccagtaatct cgaccagatt cttctcgacc ttcacaaaga cattctgagc ctgttcgatg
     2641 cggaggatct gaccctcttc gcctttgatg ccgacaagaa agaaatcttc tacaaggttc
     2701 cgcacatcga taccattgaa gaagttcgca tccccatcac cgagcaaagt ctggccggat
     2761 tctgtgcgaa atacttgcgc ccggtaaata ttgccgacgc ctacaacgtg gctgaactgc
     2821 aagggatcca tccttcgttg aatcacgata cgtcgtacga taagcgcact ggatttaaga
     2881 ccagacaggt gctgacttat ccgatcgtcg ccgacaacaa atacctgatg ggtgtgctcc
     2941 aactcctcaa caagaagagc ggcctgcgct ttacccgcaa ggatgaagaa tccgtggccg
     3001 aaatcgccaa ggcgctcggc attgcattct tcaacctcaa aaaggtctcc accaaaaaaa
     3061 cctcgaccaa gttcgacctg ctggtgacca acggcaagat cacccagaac gaactcgata
     3121 acgccatcgc cgaagcgaga aaaagcattt catccgacct tgaatcgatc ctcatcgaaa
     3181 agtacaaggt gccgaaactc gagatcggca gatccatcgc ccagtttcac aagtgtccct
     3241 acatcgaata cagcgaacgc acactggtcg acatcgaact gctcagaaat ctgaacgttg
     3301 attacctcaa aaagaaccac tggatgccgc tgaaacggga ccgcacagca atcgaaatcc
     3361 tcaccgacga tcccggcgat ctcgatcgag tcgcggacat caagcggaca ttccctggtc
     3421 tcaacatccg ttttgccgtc agcctcagac gggatatctc acaattcctg aactctgcaa
     3481 cgggcggcag cgacacgcag gccggacgga aactggatga aaacgtgtcc gacattctcg
     3541 gcgagctcgt gacggaagct caggccgagg ccatggaaga atcgggtccc ggcggagggc
     3601 tcgacgaaaa cgacagcgcg atcgtccgtt tggcgaacca aatcattgcg gacgcgtatc
     3661 ggcagactgc ctcagacatc catatcgagc catatggaga aaaacgcgaa acactggtgc
     3721 ggttccgcgt cgacggcgat tgtttcgaat atatgaagat cccgcaaagc tatcggcgcg
     3781 cgatcgtgtc acgcctcaaa atcatggcca gccttgacat tgcagagcgg cgaaagcccc
     3841 aggatggcaa gatcaagttc aaactcagtg aaacgcggga aatcgagctt cgcgtggcga
     3901 cgataccaac cgccggctac aatgaagacg tagtgatgcg tattcttgcg gccagcgagc
     3961 cgttgccgct cgataaaatg ggattttctg atcggaatct gaagtgcatt aaggacatcg
     4021 cggagaagcc ctacggcatc attctctgcg taggaccgac ggggtccggt aagacgacca
     4081 cgctgcactc ggttctcggt ttcatcaaca cccccgacat caagatttgg acggctgagg
     4141 atcctgtcga aatcacgcaa tacgggctcc ggcaggtcca agtacagccg aagatcgatt
     4201 ttaccttcgc gaaagcgatg cgcgcattcc tccgagccga tccggacgtg atcatggtcg
     4261 gagaaatgcg ggataaagaa acagccgaga tcggcattga agcgtcgctc accggtcact
     4321 tggttttaag cacactgcac accaacagcg ccgtggaaac ggtcacccgc ctgctcgaca
     4381 tgggctgcga ttccttcagc ttcgccgatg ccatgctcgg cgtactggca caacggctgg
     4441 cgcgaagggt ctgtaaagac tgcaaggagc aatatgcggg aacgaaggaa gagtatgagg
     4501 aaatgcgcct aggctacggc cctgactttt gggacaaact cgccatcccc atggacaata
     4561 cgttccgtct ctcacgcggg aaaggctgtg aaacgtgtaa ccggaccggg ttcaaaggtc
     4621 gggtcgcgtt gcacgaatta ctcttaggta ccgacaagct taagcggctc atacagacca
     4681 gggccaagac tgaagagatg gtcaaggccg ccatcgaaga cggcatgaca acgctcatgc
     4741 aggacggcat ccagaagtgt ttacagggac tcacgatgtt caaagaagtg aaagccgtcg
     4801 ccatcaagta aggagaggat ctcaactcca cggttgagat cctcttccat tcccctccgg
     4861 gcgaaccact catatgacac gaaccggtct cgtcctcaca ggacgggacc atcgctgacc
     4921 cggtgtaggc gaacgtcagg agcaggtgta tgccgagcga gaaaggaatt tcttgaggcg
     4981 agactctacc gacagaggga cagataggaa ttccagtccg aatagctgat cattgaccca
     5041 tttgacggtc gcgtcgagct gcagggcgga ggcttggtca ggcattcgaa agctgaccgc
     5101 cagatggtcg cccggtctca ttgccgcggc actcataact ttcgcgccac ccaacgagac
     5161 gtccagtaca acgcccagcc cgttgcggtc aacggcggcc cagcgtttta gcccgaggag
     5221 ggagaatgca cacgggaagg gagcggagac tcgatgacga ggatagcgtc gcagctccac
     5281 gctcggctga ggctgtcgtt ccagttcagc catggaaggt tatgcgaccg ctcgcgaggc
     5341 gatcgccacg aatctcttca atcgatcata cgcatcgagc gagaggcggc gaaatgccag
     5401 cccatagaac tgatccttcg tccatcgcac ggttgcgacg gcaatctccg cggaacggat
     5461 ttgcttcggc agacgaaggc tcaaggccac ctgatccccg ggcttgaagg gagcttccgt
     5521 gctgacccgc gcgccccgaa cagaaagatc atagaccaac cccacgttga tgatgggttt
     5581 tctcagccag cctcgcgacc ggaccgggga gagggcacaa gaaaatggtg tattgatgcg
     5641 gactcttgga tgtcgtcgaa agcgattcag ggcagacggt cgacgc
//
LOCUS       DASTQY010000496         3784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_123533, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000496 DASTQY010000000
VERSION     DASTQY010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123533"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VFR82_15880"
     CDS             complement(<1..530)
                     /locus_tag="VFR82_15880"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4503129.1"
                     /translation="MPERGIRHTVLAAVSCVIILTTFSGCGKRVAGSSGDASSTTRGK
                     STAAPVETVQPEGFATIPDDKRSTFETMRTPGDIASAPSETSPPSALTEPAPAPSSAA
                     SSPLDASNLVDIFFDYDQYAIRRDAQTILGTNADWIKNRPGKAIVIEGHCDERGTQAY
                     NLVLGDKRARSVKRYL"
     gene            complement(723..1367)
                     /locus_tag="VFR82_15885"
     CDS             complement(723..1367)
                     /locus_tag="VFR82_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503130.1"
                     /translation="MVAQGNGARGLLAALLWVGFTPAFAIDVKLSSEEAQKALEAGRA
                     PMEKANSPEEVRKVLQHASMATRVGADPEKDACGASAILRTKRYRLEAFGRQEASESK
                     KRKTDIRMPDEFIRKVVDMPNMEVEVQLCGDDEYFAEGALIELQQGSKKVKAIDVGKA
                     ERGRKNEGSGPAYRSRFTAVFAYESFDPNAASVFVINLQDGKEVRIPADFSKVK"
     gene            complement(1428..2063)
                     /locus_tag="VFR82_15890"
     CDS             complement(1428..2063)
                     /locus_tag="VFR82_15890"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEU4503131.1"
                     /translation="MRRLVLASTSPRRRDLLALLGIPFEIRDPIYEERLVSDRSADEL
                     VRSFAQRKADSVAQHAPDALVLASDTLIEVDGELLGKPGDLVDARRILRHLAGREHLV
                     KTAVTLACRGRHLETTDISSACIRMKPFDAEAHERYLTTGDSLGKAGAYSIQGPGADL
                     IESLEGDFTTVVGFPLRLVVRMLQESGMTVPVDIDMVYDRKPYSNWARFAD"
     gene            complement(2051..3226)
                     /locus_tag="VFR82_15895"
     CDS             complement(2051..3226)
                     /locus_tag="VFR82_15895"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEU4503132.1"
                     /translation="MTFWNTLPTPIIGLSPMDGVTDSAFRYTIASQGKPDVIFTEFTH
                     VHDVCRGPEFLLDSLHYDESERPIVAQLYGKDPLLFYQAAHVVCELGFDGLDINMGCP
                     SRNVASSGSGAGLIRTPDLAHAIIRAARQGIADWTAGQTLEGAGLKPVRIDLINRLKA
                     QHEPAAARARMPIPLSVKTRLGYDEVIVERWIEHLLSESPAVISLHGRTLQQMYRGQA
                     DWSAIARAAQVVRGSGTLLFGNGDVQTLSDIVRRVCETGVDGVLVGRAALGAPWFFRD
                     KEQARHCVQTDRALARPPWTPDLAARFRVLLEHARVFERQWGSRQFRRMRKHLGWYCK
                     GFPRAAALRARMFLVSSVAELEAVLADYQVSVHANDQEETSSGLFESTAPLASRCGG"
     gene            complement(3223..>3784)
                     /locus_tag="VFR82_15900"
     CDS             complement(3223..>3784)
                     /locus_tag="VFR82_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503133.1"
                     /translation="FQDAHRRFSPTIDRCLSNRILAPDTVRPADEGRNVLAACGIDRE
                     QLLADYAQYVQIASGQQPPTGPNWKEVAADTVGLTRYIHEYLPHEIAHWGGDITDMFP
                     ETVRRFNETGLSLSQFVRWWFHRPRPVDRPYDFFLLKIDLLVEFVSERSPSLLSLARQ
                     EADVLRSAYTDANAHADLEALAELRR"
BASE COUNT          792 a         1131 c         1066 g          795 t
ORIGIN      
        1 tccagatagc gcttgacgga gcgagcccgc ttgtctccta acaccaggtt gtaggcttgc
       61 gtgccacgct cgtcgcagtg tccttcaatg acgatcgcct ttcctggccg gttcttgatc
      121 cagtcggcat tggtaccaag aatcgtctgg gcatctctcc tgatcgcata ctggtcgtaa
      181 tcaaaaaaaa tgtcgaccaa gtttgatgcg tctaggggag atgaggcggc agatgaaggg
      241 gcaggtgccg gctccgtcag ggcactcgga ggtgaagttt ccgatggagc agaggcgatg
      301 tctcccgggg tgcgcatcgt ctcaaacgta gacctcttgt cgtccggaat ggttgcaaag
      361 ccctcgggct ggacggtctc cactggtgcc gctgtgcttt ttccccttgt cgtggaagag
      421 gcatcgcccg acgatcccgc cacgcgcttc ccacaacctg aaaacgtcgt cagaatgatc
      481 acacaggata cagccgctag cactgtatgc cgaattcccc gttctggcat tccccacctc
      541 tctctataga tgaatggctg cgatccaccg ttcgtgcact gcattttcga aggattggtc
      601 agaaaacagg agatttatag ccggtcgctg cgtttctcgc aaggggagaa ggaggaaagt
      661 gagccgtgat ctgatcgggg aggtcgtccc tcggtcgcac atgaggtatg cgaacgatac
      721 cgctatttca ctttggaaaa atcagcagga atgcggactt ctttgccgtc ctgcagattg
      781 atcacaaaca ccgaggcggc gttagggtcg aagctttcat aggcgaacac cgcagtgaat
      841 cgagaccggt aggcggggcc acttccttca tttttccggc ctcgttccgc tttaccaacg
      901 tcgatggctt tcactttctt gctgccctgc tgcagctcga tgagagcgcc ttccgcgaag
      961 tactcgtcat ccccgcatag ctgcacttcc acttccatat tcggcatatc aacgactttc
     1021 ctaatgaatt cgtcgggcat gcggatgtca gttttccgct ttttggactc ggaggcttcc
     1081 tgtcggccga aggcttctag ccggtagcgc ttggttctga ggatcgcact cgccccacag
     1141 gcatcttttt cgggatcggc tcccacgcgt gtagccatgg atgcgtgctg aagaactttt
     1201 cttacttctt caggactgtt ggctttttcc atcggtgcgc ggccggcttc gagcgccttc
     1261 tgcgcctcct ctgaggacag cttcacgtcg atggcgaatg ccggagtaaa acccacccac
     1321 aggagcgcgg ccagcagacc tcgcgccccg ttcccttgag ccaccatgag ggcctccata
     1381 aaaacgaatg aaggcggcat tctacgaaaa gcccctcccc tttgcaatca gtcagcgaac
     1441 cttgcccaat tggagtacgg cttcctgtca tacaccatgt cgatgtccac gggaactgtc
     1501 atgccgctct cctgcaacat gcggacgacg agacgaagag gaaaaccgac gacggttgta
     1561 aagtcaccct ccagcgattc gatcaagtcg gcgcccgggc cctgaatcga gtaggccccc
     1621 gcctttccaa ggctatcgcc cgttgtgagg tagcgttcat gtgcttcggc atcaaacggc
     1681 ttcattcgaa tgcaggcgct cgatatgtca gtggtctcta agtgtcgccc gcgacatgcg
     1741 agtgtgacgg ccgtcttgac gagatgctca cgcccggcga gatgccgcaa gattctgcgc
     1801 gcatcgacga gatcgcccgg ctttcccagc aactctccat ccacttcgat cagcgtatcg
     1861 ctagccaaga ccagggcgtc tggggcgtgt tgtgccaccg aatcggcctt gcgttgcgcg
     1921 aaagaccgga cgagttcgtc ggcggaccgg tccgacacaa gacgttcttc atagatagga
     1981 tcgcggattt caaatggaat gccgagaagg gcaagcaggt cacggcgacg cggtgaggtc
     2041 gaggccaaca ctaaccgccg catcgagatg ccaaaggggc ggtgctctca aaaagaccgg
     2101 atgacgtctc ttcctgatcg tttgcatgaa cggagacctg atagtcggca agcaccgctt
     2161 cgagctccgc gacggaggag acgaggaaca tcctggcgcg gagcgccgcg gcgcgcggaa
     2221 atcccttgca gtaccagccg agatgttttc tcatccgcct gaattgccgg ctgccccatt
     2281 gtctttcaaa tactcttgcg tgctccaaca gcacgcggaa ccgcgctgca aggtctggtg
     2341 tccacggggg ccgcgccagc gcccgatccg tctggacaca gtgccgagcc tgttccttgt
     2401 ctcgaaagaa ccacggagcc cctaacgccg cccgtccgac gagcacgccg tccacccctg
     2461 tttcgcagac acgacgcacg atgtcgctca aggtctggac gtctccgttc ccgaacaaca
     2521 aggttccgct gccgcgaacg acctgagccg cgcgcgcgat cgccgaccaa tcggcctgcc
     2581 cccgatacat ttgctggagt gttcttccat gcaatgagat gaccgcggga gactccgaca
     2641 gcagatgttc gatccaccgc tcgacgatca cttcatcgta tccaaggcgg gttttaaccg
     2701 aaagaggaat gggcatccga gcgcgagccg ccgcaggctc gtgttgggcc ttcagccggt
     2761 tgatcaggtc gattctgacc ggtttgaggc cggctccctc gagcgtctga ccggcagtcc
     2821 aatcggcgat tccttgtctc gcagcgcgta tgatcgcatg cgcgagatca ggcgtccgga
     2881 tcaaacctgc cccggatcct gaggaggcga cgtttcgaga gggacaaccc atgttgatgt
     2941 ccagaccgtc gaaacccaac tcgcagacca cgtgcgccgc ctgataaaac aaaagcggat
     3001 ctttgccgta caactgcgcc acaatcggac gctcgctctc gtcatagtga aggctatcga
     3061 gcagaaattc cggtccgcgg cacacgtcat ggacatgcgt gaactcagta aagatgacat
     3121 ccggtttgcc ctgacttgcg atcgtatacc gaaatgccga atccgtcacg ccgtccatgg
     3181 gtgaaagtcc gatgatcggc gttggcagag tgttccagaa cgtcatcgcc gcaactccgc
     3241 gagagcttca agatcagcgt gagcgttggc atcggtgtag gccgagcgca agacatccgc
     3301 ttcctgcctg gccaaggaca gcaatgaagg tgaccgttcc gagacgaact ccaccaggag
     3361 gtcgatcttc agcaaaaaga aatcatacgg ccgatcgacc gggcgaggtc tgtgaaacca
     3421 ccacctcaca aactggctta aggagaggcc ggtttcgttg aagcggcgaa cagtctcggg
     3481 aaacatgtcg gtaatgtctc ctccccaatg ggcgatctca tgcggcaaat actcatgaat
     3541 gtagcgcgtc agacccacgg tatctgcggc gacttccttc cagttcggac cggtcggcgg
     3601 ctgttgacca gatgcaatct gtacgtattg ggcataatcc gccagcagtt gttcgcggtc
     3661 gatgccgcac gccgcaagca cattccgtcc ctcgtccgct ggccgaacgg tgtccggagc
     3721 caggatgcgg tttgacaaac agcggtcgat tgtagggctg aaccgcctgt gggcatcctg
     3781 aaat
//
LOCUS       DASTQY010000497         8312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_124433, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000497 DASTQY010000000
VERSION     DASTQY010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124433"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /gene="moaC"
                     /locus_tag="VFR82_15905"
     CDS             complement(<1..384)
                     /gene="moaC"
                     /locus_tag="VFR82_15905"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEU4503134.1"
                     /translation="MAEFTHFNESGRARMVDVSAKAQTERVASAQAKVYLQPETLEKI
                     QRGKIAKGDVLAVAQVAGVMGAKKTPELIPMCHPIFLSAIDISFKEEPQPDRDGRCSI
                     TITATAKTSGQTGVEMEAMTAVSVAA"
     gene            674..1621
                     /gene="folB"
                     /locus_tag="VFR82_15910"
     CDS             674..1621
                     /gene="folB"
                     /locus_tag="VFR82_15910"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEU4503135.1"
                     /translation="MTDRIVIERLEFQGRCGITAGERRQPQPMAVDLELDCEARSAAG
                     SDHLGDTVDYAAVVERIVDLGGRESCALLESLAEKMLTMLFTEFPISRARLWLRKLAP
                     PIAHTTGSVGIKVDRGRATHHEQLIDPMPSRFVAQHLHRLPKGRALDVASGSGRHALY
                     LANHGFEVDAIDRDGESLAKLSAEAARRHLHVETKQVDLERTTDERPEYPANTYDAII
                     VCFYLHRPLFPWLIEALKPQGVLLYETFTIDNYVRYRHPRRWEFCLAQNELLRLTSSL
                     RVLSYDEGEHEGGHGSGSVFTAQLLAQKPRPAVRAHELP"
     gene            1618..2463
                     /locus_tag="VFR82_15915"
     CDS             1618..2463
                     /locus_tag="VFR82_15915"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU4503136.1"
                     /translation="MSRIDLHLHTTHSDGSLQPSEVLALAKKANVTALAITDHDITSG
                     ISEAKAAGQHLDIEVIPGVEVSSYDGRSELHILGYYVECDDSLFMSRLASLRASRHRR
                     NPLIIERLREAGLDVTYDEVRALAGTESVGRPHIAQLLMRKKYVSSAKEAFDRYLAEG
                     KSAYVARELPTPEEAIRWIREAKGVAVLAHPTWVKDTGDGLLSCITALKEAGLGGVEV
                     HYSTHSKSQTANYLELTQRLGLLVTGGSDFHGITKPDIEVGYGRGDLRVNPALLEPLQ
                     AAARA"
     gene            2583..3980
                     /locus_tag="VFR82_15920"
     CDS             2583..3980
                     /locus_tag="VFR82_15920"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU4503137.1"
                     /translation="MSKSLHWIIPHASGILAALLLGPLLGSMAWVEFFSIPLTAVTGP
                     NAVRLVTEGIALGLVMAFAVSAYEQLPDNGRGSSFLRSIVIPSAALIVVVFSAKALGS
                     VGLPLIHQMGLSAFVQGYTIVVVACGLWLTIAWLRHMHLLRNMFSPPAVKRTLARAAE
                     EEEPLLSDSHNEIAEQAKEKVAAIVLNGHPPTSLGRYKILKELGRGAMGLVYLGKDPT
                     IQRFVAIKTMRLDQIDDSEKLQEIRGRFFREAESAGRLSHPNIVTIYDAGEQHELGYI
                     AMELVEGRSLKEWSRKPHLMPLPELIPTLASVAEALDYAHQQGVVHRDIKPANIMITK
                     ERLVKVMDFGIAKMTSNSKTQTDVVLGTPTYMSPEQIAGKKVDGRSDVFSLGVVLFEL
                     LTGQPPFTADNLSALLFAIAHNPHPELHMLRHDLPPMFEEVLNRALQKELPQRYRRAG
                     ELAQDLRSCLQSLAA"
     gene            3992..4816
                     /locus_tag="VFR82_15925"
     CDS             3992..4816
                     /locus_tag="VFR82_15925"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU4503138.1"
                     /translation="MAITRLHFAETDIGRKRSHNEDCYAAEPALGLYVVCDGMGGGNA
                     GEVASHMAIDAILAYVKAEARRGCVAGRATVNPALSPLTSQLADAIRAANETVYRASR
                     EYAKYAGMGTTVVAARLTGRHVSIAHVGDSRLYLIRNGAIQTLTSDHSWVAEQVAKGY
                     MTEEEAERSPRRNIVTRALGIESSVDIDLAEIPVSNGDLLLLCSDGLTRGVRCHDILR
                     TVDAAGDLCDKTHRLVSLANDAGGDDNITVLLVSVQESAAGRLWHRLAPRWLLKAS"
     gene            complement(4911..5513)
                     /locus_tag="VFR82_15930"
     CDS             complement(4911..5513)
                     /locus_tag="VFR82_15930"
                     /inference="COORDINATES: protein motif:HMM:NF013035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HEU4503139.1"
                     /translation="MSEETLSNRILAGALTFIVLGLIWVAIVSSLPSEQELAAKEAPI
                     LPRAVSRSLPEPTLDLSIPGVPATQPARAPLSTVAVEKPFASKTLDPRAAQITRLRCE
                     AEVEQLCPESLDGTARKQCLEQRTPQLPVSCQHQVRQRLVKWKEERSRLTVACQIDIK
                     RFCPMKPGSGELLQCLQQHAQELSDGCYERLPKGALHLKH"
     gene            complement(5689..6702)
                     /locus_tag="VFR82_15935"
     CDS             complement(5689..6702)
                     /locus_tag="VFR82_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503140.1"
                     /translation="MQSWSRPDPLTRDLIHLINARRHDHGDTDAAIDTLQAFLEQGRE
                     DDLLTVLAALDEDMAEWLFDLVAEASCSIMLDGPADEKLSYAVMAALELPLQANLTHP
                     LKLKIDPVATAEVLHRHLRLSSGTVVRVEPRLLTDATLEALSLQTLNEHLSTVAESQR
                     GRSIAARLYPPVENTAFLFFELIGVPDAGLPDSLEDPHRRALLEGLVEQCPELALAPD
                     TIEAPVYAAYAMFDAQNAVRLHHLADETAAVWRDLDPLVRARTIVHLHTQCGNGVYMP
                     VWTDLFDPELPEEHGPVWTSPDVWVFTADLPIEWPGEMLTNRLLAEGCTRFENHIVEA
                     PEN"
     gene            complement(6815..7249)
                     /locus_tag="VFR82_15940"
     CDS             complement(6815..7249)
                     /locus_tag="VFR82_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEU4503141.1"
                     /translation="MHSGPSAMRSYREELWFETKTRRAYLNITRQVESVVKNSGVQEG
                     LVLVNAMHITASVYINDDEPGLLADYDRFLERIAPQDDVYRHNETGEDNGDAHIKRQV
                     MGREVVVAISNGRLDFGPWEQIFYGEFDGRRRKRVLVKVIGD"
     gene            7414..>8312
                     /locus_tag="VFR82_15945"
     CDS             7414..>8312
                     /locus_tag="VFR82_15945"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HEU4503142.1"
                     /translation="MLRGRYLTKNDHGTVTETVPELFWRIATDIAQAEQLYPGPTRLV
                     HAARRFYESMARLEFLPNSPTLMNAGRPLQQLAACFVLPVEDSLSSILEAVKQQALIH
                     QSGGGTGFSFSRLRPQDDLVSSTQGIASGPVSFMRIFNMATDVIKQGGTRRGANMGIL
                     RVDHPDILEFIGLKQDQSAMNNFNLSVGITSDFMRALARNRHYPLVNPRTRKPTKRLP
                     AAMVFDRLVQAAWATGEPGVIFLDHINADNPTPRLGDIEATNPCGEQPLLAYESCTLG
                     SINVSKFVRPGRPAPVIDYERLAT"
BASE COUNT         1736 a         2479 c         2365 g         1732 t
ORIGIN      
        1 agcagcgaca gagacggcag tcatcgcttc catctcgacc cctgtctgcc cgctcgtctt
       61 ggctgtggcc gtgatggtga tagagcagcg tccatcacga tcgggttgcg gttcttcctt
      121 gaacgagata tctatcgccg agagaaagat cggatggcac atgggaatca actcgggggt
      181 ctttttggcc cccatgacgc ctgccacttg ggccacggcg agtacatccc ctttcgcaat
      241 ctttccgcgt tgtatctttt caagagtttc gggttggagg tagaccttcg cctgggcgga
      301 cgccactcgt tcggtctggg ccttggcgct cacatcaacc atccgcgccc gacccgactc
      361 gttaaagtgt gtgaactcgg ccattttcct cggcgaatca gccggttaga atgtgaagac
      421 cggtcattat atgaagggct ccaatggcgc gtcaaggcgt gagtaccaat ccaactcgct
      481 aggctcggag ccctcaggcg atcgtcctct agaattcaga cctgaatgaa ttccttgttt
      541 tccctccact tggcgacaac aggcgtcctc cctacgttga gagatgacgg ctcggtgctg
      601 cctgcggaat actgacctcc cccttcctac gacaggttga cagcttctct tggcatcccg
      661 gagaatgcca agaatgaccg acagaattgt gatcgaacgg ttggaatttc aagggcgatg
      721 cggcattact gccggggagc ggcgacagcc tcagcctatg gccgtcgacc tcgagctgga
      781 ctgtgaagcc cgatccgcag cgggttcgga tcatttgggc gacaccgtcg attatgccgc
      841 ggtcgtcgaa cggatcgtcg acctgggagg acgagagagc tgcgccctac tcgagagcct
      901 tgccgaaaag atgctgacga tgttgtttac ggagtttccg atcagtcgcg cgcgcctctg
      961 gctccgtaag ttagcccctc caatcgctca taccactggg tcggtaggca tcaaagtgga
     1021 ccggggccgt gccacccacc atgagcaact catcgatccc atgccatccc gcttcgttgc
     1081 ccagcacctg caccgcttac caaaaggccg agcattggat gtcgcgagcg gaagcgggcg
     1141 ccatgctcta tatctcgcca accacggttt cgaagtcgac gccattgacc gcgatggcga
     1201 gagtctcgct aaactgtcgg ccgaggccgc caggcggcat cttcatgtcg agaccaaaca
     1261 agtcgacctc gaacgaacaa ccgacgagcg accggaatat ccggccaata cctacgatgc
     1321 catcatcgtg tgtttttatc tgcaccggcc gttatttccc tggctgattg aggccttgaa
     1381 gccgcaggga gtactccttt acgaaacctt caccatcgat aactacgtgc gttatcgcca
     1441 tccccggcgc tgggagttct gtctggcgca gaacgaactg cttcgtctga cgtcttccct
     1501 ccgtgtactc tcgtacgacg aaggcgaaca tgagggaggt cacggatcgg gctccgtctt
     1561 tactgctcaa ctgcttgccc aaaaacccag accggccgta cgggcgcacg agttgccgtg
     1621 agtcgaatcg atctccacct tcataccacc cattcggacg ggagcttgca gccgtctgaa
     1681 gtgttggcgt tggccaagaa agccaatgtg acagcgttgg cgatcacgga ccacgacatc
     1741 actagcggca tttccgaagc caaggccgcc ggccaacacc ttgacatcga agttatcccc
     1801 ggggtcgaag tcagctcata tgacggtcgc agcgaactgc acattttggg ctattacgtt
     1861 gagtgtgacg acagcctatt tatgagccgg ctggcttccc ttcgtgccag ccggcatcga
     1921 cgcaatccgc ttatcatcga acgtcttcgt gaagccggtc tcgatgtgac gtatgatgag
     1981 gtgcgggcgc ttgccgggac cgaatcggtc ggacgacctc atattgctca gctgctcatg
     2041 cggaagaaat atgtgtcgtc agccaaggag gccttcgacc ggtatcttgc cgaagggaaa
     2101 tcagcgtacg tcgcccggga actgcctacg ccggaagaag cgatccgatg gattcgcgag
     2161 gccaaaggcg tcgccgtgtt ggcgcatccc acatgggtga aagacaccgg cgacggactt
     2221 ctctcctgca tcaccgccct gaaggaggca ggcctaggcg gggtcgaggt gcactacagc
     2281 acacacagta aatctcaaac ggccaattac ctggaattga cacaacgtct cggcttgctc
     2341 gtgaccggcg gcagcgactt tcatgggatc acgaagccgg atattgaggt tgggtacgga
     2401 cgtggggatc tccgcgtgaa tcccgcgctg ttggagccgc tccaagccgc cgcaagagcc
     2461 tgaggtaccc tgctcagaac cgtcctctca cacccgtcca tccccgcaat tttcctggta
     2521 ttcattgact cttcagctcc gtccgttaga ctgaggacca ttcaatcgtc tcgaccaaaa
     2581 ccatgtcaaa aagcttgcat tggattatcc ctcacgcgag cggcattctc gcggctctac
     2641 ttctcggccc ccttctcggc agtatggcct gggtcgaatt cttcagcatc cccttgaccg
     2701 cggtaaccgg accgaacgcg gtgcggttgg tgaccgaagg gatcgcactg gggctggtca
     2761 tggcttttgc ggtctctgct tatgaacagc ttccagacaa cggccgcgga tcaagctttc
     2821 ttcgctccat cgtcattccg tcagccgcgc tgatcgtcgt cgtctttagc gctaaagcgc
     2881 tgggctcggt cggtctgccg ttgatccatc agatggggct gtcggcattt gtgcaaggct
     2941 atacaatagt agtcgtcgcc tgcggactgt ggctgacgat agcctggctc cgacatatgc
     3001 acctcttgcg caacatgttt tcgccgcccg cagtcaagcg aactctcgcg cgagcggcag
     3061 aagaagagga gccactcctg tcggacagcc acaatgagat tgccgagcag gcaaaggaga
     3121 aagtcgcggc catcgtcctc aacggccacc ctccgacaag cctgggccgc tacaaaatcc
     3181 tcaaggaatt gggacggggt gcgatgggcc tcgtgtatct cggcaaagac cccacgattc
     3241 aacgcttcgt cgccatcaag accatgcggc tggatcagat agatgactcg gagaaactgc
     3301 aggagattcg gggacgtttc ttccgcgaag ccgaatcggc gggacgcctg tctcatccca
     3361 acatcgtcac catttacgat gccggtgaac agcatgaact gggctatatc gcgatggagc
     3421 tggtcgaagg ccggtcgctg aaagagtggt cgcgcaaacc gcatctcatg ccgctccctg
     3481 aactcatccc gacgctcgcc tccgtcgctg aggccctcga ttatgctcac caacagggcg
     3541 tcgtccaccg cgacatcaaa cctgcgaaca tcatgatcac caaagaacgc ctcgtgaagg
     3601 tcatggactt cggcatcgca aaaatgacat cgaacagcaa gacacagacc gatgttgtcc
     3661 tgggcacgcc cacctacatg tcgcccgagc agatcgccgg gaaaaaagtc gatggacgtt
     3721 ctgatgtgtt ttcactcggc gtcgtgctct tcgagctgtt gaccggacaa ccgccgttca
     3781 cggcggacaa cctgtcggcc ctgctgttcg ccatcgcgca caatccacat cccgagcttc
     3841 atatgctgcg ccacgacctg cctcccatgt ttgaggaagt gttgaaccga gccttgcaaa
     3901 aagagctccc ccagcgctat cgccgggccg gagaactcgc gcaagatctc cgctcctgtc
     3961 tacagagtct cgccgcctag gaggagggct tgtggcgatc acccggcttc acttcgccga
     4021 aacggacatc ggacgcaagc ggtctcataa cgaagactgc tatgcggctg agccggcact
     4081 cggactctat gtcgtctgtg acgggatggg gggcggcaat gcgggcgaag tggcaagcca
     4141 catggcgatc gacgccatcc tcgcctacgt gaaagccgag gctcgaagag ggtgtgttgc
     4201 cggtcgagct acggtcaacc ccgctttatc tcccttgacc agccagctgg ccgatgccat
     4261 ccgcgccgca aatgagacgg tgtatcgagc gtcaagggag tacgcgaagt acgccgggat
     4321 gggtaccacc gtcgtcgccg cccgccttac cgggcggcat gtgtccattg cccacgtcgg
     4381 agatagtcgg ctgtacttga tccgtaacgg tgcaatacag acgttgacca gcgaccattc
     4441 gtgggtagct gaacaagtgg caaaaggcta catgaccgaa gaggaagcgg agcgttcacc
     4501 gcgccggaac atcgtcactc gagcgttggg catcgaaagc tccgttgaca tcgatctcgc
     4561 cgagatccct gtctctaacg gtgacctcct gctgttgtgc tcagacggtc tcacccgagg
     4621 cgtccggtgc cacgacatcc tcaggaccgt cgatgcggcc ggcgatctgt gcgacaaaac
     4681 gcaccgattg gtctcgctgg cgaatgacgc cggcggagac gacaacatca cggtgttgtt
     4741 ggtcagcgtc caagaaagcg cggctggtcg gctgtggcac cggctcgcgc cacgctggtt
     4801 gttgaaagcg tcttgacctt caccggagcg tgtacttacg ggcattatca tcgaggtggc
     4861 cggcgtctcg ttgacgttct atgtcgtccc cgaatgccag atagcggatc ctagtgcttg
     4921 agatgcagcg ccccttttgg aagcctctca taacacccgt ccgatagttc ctgggcgtgt
     4981 tgctggagac actgcagcag ctccccgctt cccggtttca tgggacaaaa tcgcttgata
     5041 tcgatctgac acgcgaccgt caagcggctc cgctcttcct tccatttgac caagcgttgg
     5101 cgcacctgat gctgacaaga cacgggaagt tgcggcgtcc gctgttcaag gcattgttta
     5161 cgggcggttc catcgagaga ttcggggcag agctgttcga cctcggcttc acaccgcagt
     5221 cgagtgattt gtgcagcacg aggatcaagc gtcttggagg cgaacggctt ctctacagca
     5281 acagtcgaca gcggagctct ggcaggctga gttgccggca cgccaggaat cgacagatcg
     5341 agtgtgggtt caggaaggga cctagacact gcccgcggca agatcggggc ttccttcgca
     5401 gcgagctcct gttctgaagg cagactgctg acgatggcaa cccagatcag ccccagcacg
     5461 atgaaggtga gcgcgccggc cagaattcgg ttcgaaagcg tttcctcgct catggtttgt
     5521 cccttgccca atagaattca gcatagggca aggcacacaa aaaatcgacg aaatgtgggg
     5581 attcggcaag gagtagacag aattgtcaga aaggaaacag ttatctttcc gctgttggac
     5641 gggcagccca attaacttgg tttcaggaga actcgggaca ggcaccctct agttctccgg
     5701 ggcttcgacg atatggtttt cgaacctggt gcaaccctct gccaatagac gattggtgag
     5761 catttcacct ggccactcga ttggcaggtc ggcggtgaat acccagacat ccggcgaggt
     5821 ccagacaggg ccatgctctt caggcagctc cgggtcgaac aaatccgtcc acaccggcat
     5881 atagacgccg tttccgcatt gggtatgcaa gtgaacgatc gttctcgcac ggaccagcgg
     5941 atcaaggtcg cgccagacgg ccgctgtttc gtcggccagg tgatgaagcc ggacggcgtt
     6001 ttgcgcatcg aacatggcat aggctgcata caccggcgcc tcaattgtat ccggagcgag
     6061 cgccagctca ggacattgct cgactaatcc ctccaggagc gcccgacgat gagggtcttc
     6121 aagggaatca ggtaggccgg catccggcac cccgatcaac tcaaagaaga gaaaggcggt
     6181 gttttcgacg ggtggataga gccgagccgc aattgaacgc ccacgctgcg actccgccac
     6241 cgtgctgaga tgctcattca aggtttgcag actcaaggct tcgagggtgg catcggtcaa
     6301 taagcgcggt tcgacgcgaa cgaccgttcc tgaggacaac cgcaaatgcc gatgcaacac
     6361 ttcggccgtc gcaaccggat cgattttgag ctttaatgga tgggtgagat tggcctggag
     6421 cggaagctcc agcgcggcca tgaccgcgta ggacagcttt tcgtccgctg gtccgtcgag
     6481 catgatggaa caagaggctt cagcgacaag atcgaagagc cattcggcca tatcttcatc
     6541 cagcgcagcc aacaccgtca acaagtcatc ctcgcggcct tgctcgagaa aggcctgtaa
     6601 ggtatcgatg gcggcatccg tgtcgccgtg atcgtgccgg cgcgcgttga tgagatggat
     6661 cagatctctg gtcaggggat cgggacgaga ccaactttgc atgcgacgct ccagcactca
     6721 ccaacaggtg ttgactccca tgttgccaaa ctttccatca gggagcaagc gcccaactgc
     6781 atttggaccg agcggcagat tgcgcctcgg catgtcagtc tccaatgact ttcaccagca
     6841 cgcgtttgcg acgccggccg tcgaattcac cgtaaaaaat ttgttcccat gggccaaaat
     6901 ccaaccggcc gttcgagatg gcgacgacca cttcccgtcc catcacctgc cgtttgatgt
     6961 gagcatcgcc gttgtcctcg ccagtttcat tgtggcgata cacgtcatcc tggggcgcga
     7021 tgcgctccaa aaagcggtcg tagtctgcaa gcagccctgg ctcgtcatcg ttgatgtaga
     7081 cgctcgcggt aatgtgcatg gcgttgacca gcacgagacc ttcttgcaca ccgctgttct
     7141 tgacgacaga ttcgacttgc cgcgtgatgt tgagataggc gcggcgggtc tttgtttcga
     7201 accagagctc ttcgcggtac gatcgcatgg cggatggacc ggagtgcatt ctgcgtctca
     7261 acagcctgaa gatgcaatcc cccgcatcgg gcggagcata tcgtcaaacc cggccggcgg
     7321 cgattataat atcgcatgcg ttccagaccg tctttgccat tacccgcgcc gccgaaatca
     7381 cctgttccgc ggctgtcggc agaagcgaag ctcgtgcttc gtggacgtta tttgacgaag
     7441 aatgaccacg gcacggtcac tgagaccgtt cctgagttgt tctggcgcat cgccacagac
     7501 atcgcgcagg ctgagcagct ctatcccggc cccaccagac tcgtgcatgc ggcgcgccgc
     7561 ttttacgaat ccatggcccg gctggaattt cttcccaatt cgccgacgct catgaatgcc
     7621 ggacgaccgc tgcaacaatt ggccgcctgc ttcgttctgc cggtcgagga ctctttgagt
     7681 tcgattctgg aggcggtgaa acagcaagcg ttgatccacc aatcgggcgg cggcaccggt
     7741 ttctccttca gccgccttcg gccgcaagac gacctggttt cctcgactca gggtatcgcc
     7801 tcggggcctg tgtcattcat gcgcatcttc aatatggcaa cggacgtcat caaacagggc
     7861 ggtacgcggc gtggcgcgaa catgggcatc cttcgtgtcg atcatccgga tattttggaa
     7921 ttcatcgggt tgaagcagga tcagtctgcc atgaacaact tcaatttgtc cgtgggcatc
     7981 acgtcagact tcatgcgtgc gctggcacgt aatcgacact atccgctcgt caatccgcgg
     8041 accaggaaac cgaccaagcg attgcccgcc gcgatggtgt tcgaccggct ggttcaggct
     8101 gcctgggcta cgggagagcc gggcgtcatt ttcctggacc acatcaatgc cgacaatcca
     8161 acaccccggt tgggagacat cgaagccacc aatccctgcg gtgagcaacc gctcctggcg
     8221 tatgaatcct gcactctcgg ttcgatcaac gtatccaagt tcgtccggcc tggccgccca
     8281 gcccctgtca tcgactatga gaggttggcg ac
//
LOCUS       DASTQY010000498         4349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_793917, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000498 DASTQY010000000
VERSION     DASTQY010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793917"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VFR82_15950"
     CDS             <1..629
                     /locus_tag="VFR82_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503143.1"
                     /translation="EPEVLRGLRALASSQTAMRDTLLRMQERCDPYVYYTRVRPYIHG
                     WKNSPSLPDGLSYTGLSTYAGPQQFRGETGAQSSIVPSLDAGLGIRHSPDPLTVYLQE
                     MRDYMPPLHRAFLQRLEQATDDLDRPLLAGYVQDRKSSRSELWAAYCDCVDLLAQFRE
                     IHIGYADSYIYRQHQSHVSNPTAVGTGGTPFMAYLQKHLDETKQATTD"
     gene            complement(672..1652)
                     /locus_tag="VFR82_15955"
     CDS             complement(672..1652)
                     /locus_tag="VFR82_15955"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4503144.1"
                     /translation="MNGAHSETSKPNTSFIPLACLLGVVVLLSSVTPVVKYIFQHSTF
                     QPIGLAYSRVVIGFVFLLAITWLCDRRGLMSLATADLIRLGCVGFLGVFSYAIAAWGL
                     MHTSVIHYAFIYGLLPSCTAMLSVLVGKDRMTGPKCAGIVLSLAGCVVAISWLTPFNA
                     AIQLGDLFVLLFTVMMSAHIVFTSGMVKRFGVMVSNTVMFGSSSVLLFFGSLGWSESD
                     HQGVLSPLIALLVLYVGCATAAVFILRCRSLQSLSPAIVGTYHNFIPVCTALLAYLCL
                     GEPIGSATIFGGMMVIAGAEMVRRPQILFWRFLPWTKTSLSSLESSKNPS"
     gene            complement(1658..1981)
                     /locus_tag="VFR82_15960"
     CDS             complement(1658..1981)
                     /locus_tag="VFR82_15960"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU4503145.1"
                     /translation="MGAQRQLFRIMIDRAGEIGRGNEKFPCKVIDLTEKGFQLESNGG
                     FRVGDELHLEFVLSEPNPLTCTVRVTHIRHSFLGAEILRISPDHQAELTSFIDQLNAL
                     NMTGF"
     gene            2167..2292
                     /locus_tag="VFR82_15965"
     CDS             2167..2292
                     /locus_tag="VFR82_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503146.1"
                     /translation="MSVMCSLAACKAQAGMCGHEKAMLSVALLAAIGFGAYFLMS"
     gene            2340..3326
                     /locus_tag="VFR82_15970"
     CDS             2340..3326
                     /locus_tag="VFR82_15970"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEU4503147.1"
                     /translation="MPSSRPSLAGHPSLSDPLLARTLVLDELFDLSLYQALRNVTNPE
                     VHHTLDELIAVETGHLAFWKNFFNLTLDELNPGRRLKLSLLMFVCRLFGSTAVHLVLE
                     AIEVHGIRKYLALWREYQGQPLGEALHGILMDEFKHEDVLVTALTERKINAEKIRNIF
                     LGLNDGLVEILGAVSGFFGAFGDATTVLVAASTTAVAGALSMAAGTFLALSSEKEVKS
                     AEIAKKIFLGEASEVVPMEESPIGSALVVGAAYIAGAIVPVLPVLIGATNAVFSIITA
                     GLMVVFVSTVLSFLSGMDVKRRILLNLVMIAIAVSVTYGIGVFAKTLWGIAL"
     gene            3375..4163
                     /locus_tag="VFR82_15975"
     CDS             3375..4163
                     /locus_tag="VFR82_15975"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEU4503148.1"
                     /translation="MAKAHVGLSGYSYKPWQGPGRFYPPILKQKEFLRYYATRYDTVE
                     LDGVWYRLPSEKAVSEWIDSTPSHFLFAPKVHRQITHRARLKPDCYEFVQVLLSRLMQ
                     LASAERLGPLLIQLPPNLIRDDDRLARFLEQLPSGTRWAIEFRHDSWYDTAIEAVLKG
                     FNVSWVAADTDERPAECRDTADFWYVRLRRSAYDEQDLKLWSERIGKMIDRGKSCYVY
                     CKHEDEGSPWVWADKLLQLSREWPDREDHRAGTMGISLRCSESR"
     gene            complement(4112..>4349)
                     /locus_tag="VFR82_15980"
     CDS             complement(4112..>4349)
                     /locus_tag="VFR82_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU4503149.1"
                     /translation="KDVLYLIFKPFLGQKKGATKAEVLSRAVEHAEMKDAAAQLTQLS
                     DERLLEVLETSRQERVRLQRDSEQRREIPMVPAR"
BASE COUNT          946 a         1260 c         1205 g          938 t
ORIGIN      
        1 gagagcctga ggtgctcagg ggcctccgcg cgctcgcctc gtcccaaacc gccatgcgag
       61 acacgttgct tcgcatgcaa gaacgttgcg atccgtatgt gtactatacg cgcgtgcgac
      121 cctacatcca cgggtggaag aacagtccgt cgttgccgga tggtttgtcg tataccgggc
      181 tgtcaaccta tgccgggccc cagcaattcc gaggtgaaac cggcgcccaa agttccattg
      241 tgccgtctct cgacgctggt cttggcatcc gccacagccc ggatcccttg actgtgtatc
      301 tgcaggagat gcgcgattat atgcctcccc tccatcgggc ctttctgcag cgccttgaac
      361 aagcaaccga cgatctcgac cggccgctcc ttgccggcta tgtgcaagac cggaagagca
      421 gtcgctccga gttatgggcc gcctactgtg attgcgtcga tctgcttgcg cagtttcgcg
      481 agatccacat tggttatgct gatagctata tttaccgaca gcatcaatcc cacgtcagca
      541 acccgacagc cgtcgggacc ggcggcactc cgtttatggc ctaccttcag aagcacttgg
      601 acgaaacgaa gcaagcgacg acagactaac gtccggattt aggttcaagc gggaacctgc
      661 acggcacctg ttcagctagg gttcttgctg ctttccagac ttgagagcga ggtcttcgtc
      721 caaggcagga accgccaaaa cagaatctgc ggccttctga ccatttcggc accggcaatc
      781 accatcatgc ctccaaaaat ggtagcgctt cctatcggtt cgcccaaaca caggtatgcc
      841 aggagcgcag tacagacggg aatgaaatta tggtaagtcc ctacaattgc aggagatagc
      901 gactgcaagg accggcagcg gaggatgaag acggctgcgg tggcgcagcc gacatagaga
      961 actaacagcg caatgagggg cgacaacacc ccctgatggt ccgactcgct ccaaccgagc
     1021 gaaccgaaga acaacaatac agaactgctg ccgaacatga ccgtattgga aaccatcacg
     1081 ccgaatcgtt tgaccatccc ggaggtgaac acaatgtgcg cagacatcat gaccgtgaag
     1141 agaagcacaa agagatcccc gagttggatc gccgcattaa agggagtcag ccacgagatt
     1201 gcgacgacac agcccgccag tgagagaaca atgcctgcgc atttgggccc cgtcatccgg
     1261 tcctttccga cgagcacact caacatcgcc gtacaggacg gcaacagacc gtaaatgaat
     1321 gcatagtgaa tcacactcgt atgcatgagg ccccacgccg caatcgcgta cgagaaaacg
     1381 ccgaggaaac ccacacatcc cagtcggatc agatcggcgg tcgccaatga catcaaccct
     1441 cgccggtcac acaaccatgt gatggccaga agaaacacaa atccgatcac aactctggaa
     1501 taggccagac caatcggctg gaaggtgctg tgttggaaga tgtacttcac gaccggtgtc
     1561 accgaactca acagcaccac gactccgagg agacaggcca gcggaataaa ggaagtgttc
     1621 ggcttcgaag tttctgagtg cgccccattc atcacgatca aaatcccgtc atgttcaggg
     1681 cgttcaactg gtcaatgaag ctggtaagtt cggcttggtg atcaggagag attcggagaa
     1741 tctcggcacc cagaaaggaa tgccgaatgt gagtgacgcg aaccgtacat gttaagggat
     1801 tgggttcgct gagaacgaat tccagatgca actcgtctcc cacgcgaaag ccgccgttgc
     1861 tttcgagttg aaatcccttt tctgtgaggt ctatgacttt gcacggaaat ttctcattcc
     1921 cgcggccgat ctcaccggcc cgatcgatca tgatgcgaaa gagttgtcgt tgcgccccca
     1981 tatcttcgtt cctcatcgat gcggcccata cacagccttc aaagtatagc agctgccttt
     2041 acgccgccct gagcagcgga tttttcgccc tgggagggac ctagctcgcc tgtggcaaac
     2101 ctcgtggttg actaggtccc ctaaccggcg tatcgttccc tcattcctga ggaaggagca
     2161 ctaaccatgt ctgttatgtg ttccctcgcg gcctgtaagg ctcaagccgg aatgtgcggg
     2221 catgaaaagg cgatgctcag cgtcgccttg cttgccgcca tcggctttgg cgcctacttt
     2281 ctcatgagct gacggcccgg cttcgacagt cctctcaatg acgcggcagg gagtggtgtg
     2341 tgccctcatc acgaccttct ctggcaggtc atccttcgct gtctgatccc ctcctggctc
     2401 gaacactcgt cctcgatgag ctgttcgacc tgtcgctcta ccaagccttg agaaacgtca
     2461 caaatcctga agtccaccac acgctggatg aactgattgc ggttgaaact ggccatctgg
     2521 cgttctggaa gaattttttc aatctgacgc tcgacgagtt gaatccggga cggcggctga
     2581 agctctcgtt gctcatgttt gtgtgtaggt tattcggctc caccgccgtg catttggtgc
     2641 tcgaggcaat cgaggtccac ggtatcagga agtatctggc cctttggcgc gagtaccagg
     2701 gccaacccct tggtgaggct ttgcatggca ttttgatgga cgaattcaag catgaggacg
     2761 tcctggtgac ggcactcacc gagcggaaaa tcaacgcgga gaaaatccgc aatatttttc
     2821 tgggattaaa tgacgggctg gtcgaaatcc tcggcgctgt cagcggcttt tttggcgcat
     2881 tcggagatgc gaccacggtg ctggtggcag catccacgac cgccgtagca ggagcacttt
     2941 caatggctgc cggtaccttc ctggctctga gcagcgagaa ggaggtcaag agcgccgaga
     3001 tcgccaaaaa gatctttctc ggagaagcct cagaagtggt gccgatggaa gagtcaccca
     3061 tcggctcggc gctcgtcgtc ggcgctgcct acattgcggg tgcgatcgtc ccggtcttgc
     3121 cggtcctgat tggggcgacg aacgccgtgt tttccatcat caccgccggt ctcatggtcg
     3181 tctttgtatc gacagtgctg tccttcctgt ctggcatgga cgtgaagcga cgcatcctcc
     3241 tcaacctggt gatgattgcc atagcggtca gcgtcactta cggcatcggg gtcttcgcca
     3301 agaccctctg gggcattgcc ctgtagcgag cccgccctgc tccggtagac ttgaggctac
     3361 aggggcattg atgtatggca aaggcgcacg tcgggctttc cggctattcc tataagccct
     3421 ggcagggtcc tggccggttt tatccgccca tcctcaaaca gaaagagttt ctccgctatt
     3481 acgcgacgcg ttacgacaca gtcgaattgg acggtgtctg gtatcgcctg ccttccgaaa
     3541 aggccgtctc cgagtggatc gattcgactc cttctcactt cctctttgca cccaaagtac
     3601 accggcaaat tacgcatcgc gcgcgcctca agccagactg ttacgagttc gtgcaggtcc
     3661 tcctgagccg ccttatgcag ctggcgtccg ctgaacgtct cggtcctctg ctcattcagt
     3721 tgccgcctaa cctgatccgt gacgacgacc ggctcgctcg ttttctcgaa caactgcctt
     3781 caggcactcg gtgggcgatc gagtttcgtc acgattcgtg gtacgacacg gctatcgagg
     3841 cggtgctcaa gggcttcaac gtctcatggg ttgccgccga cacggacgaa cggccggcgg
     3901 aatgccgtga caccgcagac ttttggtacg tacgcctgcg gagaagcgcc tacgacgagc
     3961 aggatctgaa actctggtct gaacggatcg ggaagatgat cgaccgggga aagagctgct
     4021 atgtctattg taaacacgag gacgaaggct cgccgtgggt ctgggcggat aagttattgc
     4081 aattgagccg ggaatggccg gatagagagg atcaccgggc gggaaccatg ggaatttctc
     4141 tgcgctgctc ggagtcccgt tgaaggcgca cacgttcctg ccgagacgtt tccagcacct
     4201 caagcagccg ttcgtcgctc agttgcgtca gctgcgcggc tgcgtctttc atttcggcgt
     4261 gctcgacagc ccgcgacagc acttccgcct tcgtcgcgcc tttcttttga ccgagaaacg
     4321 gcttgaagat gagatacagg acgtctttg
//
LOCUS       DASTQY010000499         3343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_268896, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000499 DASTQY010000000
VERSION     DASTQY010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268896"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VFR82_15985"
     CDS             complement(<1..949)
                     /locus_tag="VFR82_15985"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503150.1"
                     /translation="MTSQNSRSEILRVSGVSIFIASFFLAVSINGAFASEQQQTVIAW
                     PEKGLGLSIDLSGFKIDIDQIKPDGRRYLMASHPTTGVNVSVTLEKVAGQASTEGCSA
                     HLQRLRNQPPVSKGQDARFVTIAKMPALAYTFREFQGIRLDQRNVRACLAKENVYADL
                     HLSKVKYTPSDESLFQSVLQTVRFQYADGQNVQAQSAARQSSMQLFRAGSAYYLQSKY
                     DQAIPFYQKAYESEKQQRQLDQTLWRVLVDNLGMAYGMTGRLKEAKTIFEEGIRVDPT
                     YPMFHYNLACAFAELNDMDHAMQSLKTAFSYRKNQNAGEQ"
     gene            complement(1050..2180)
                     /locus_tag="VFR82_15990"
     CDS             complement(1050..2180)
                     /locus_tag="VFR82_15990"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU4503151.1"
                     /translation="MSNTTWAGYYLDGKSPIRQPAKIALSADSLNITTNTGQVFHWPF
                     EAIQQTQGSYAGEPVRLERVSTLEALVISSSDVLTELHNRSTRKHWHIHNPSQRQKRM
                     RWTVVAAVTAICLAFVLYRWGIPMLSVVVASTVPVSWEQRIGEEAVLYVAPQERRCTD
                     PHRTQLIQNILTSLTEAAPKSPYQFHLYVVDSPLVNALAAPGGHIVVYRGLLERTDSP
                     EQLAGVLAHEVQHVLKRHSTRMITEQVLTGVLLSTLAGDFSSVMTYGLQSAQAVGQLQ
                     YSQGHETEADEEGMRLIIKAGLMPEEMINFYRIIGELHDRESGVLDYLSSHPNWNQRI
                     ARLRAIAGHSSKGTRRLLTDVDWKFVRGACSKRGASTPLPTS"
     gene            complement(2186..3205)
                     /locus_tag="VFR82_15995"
     CDS             complement(2186..3205)
                     /locus_tag="VFR82_15995"
                     /inference="COORDINATES: protein motif:HMM:NF017770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HEU4503152.1"
                     /translation="MSLPETRTCPSFHGKVSTLFGIHIVNLALTLLTLGLYHFWAKVK
                     VRKYLFSQTEFASDRFVYHGSGKELSVGFLKAATLFGFPYVALNTVPQLVDLPWWAVM
                     ACSSLAVLVPWVFAPIAMIGSRRYRLSRTSWRGIRFSFHGKVRPFLWLTLKGTALTAI
                     TLGAYYPYYQTKRQAYLVSYSRFGNRTFSFDGIGAELAPGFVLTPLVTFFTFFIPVVF
                     AYNVNMLFLLLLPFTVAPPWLWFLAKKQRFFWDHTKLGEARFRSTITARAFLNLHAGN
                     LLLLVVTLGFAWPWTTVRTIQFHLNNLSLLGSLDEASIFQESDVAPVTTEGLANLLDS
                     GFEFD"
BASE COUNT          816 a          828 c          920 g          779 t
ORIGIN      
        1 cttgctcacc ggcattctgg tttttgcgat acgagaacgc cgttttaagc gactgcatag
       61 cgtgatccat gtcgttaagt tctgcgaagg cacaagcaag attgtagtgg aacattggat
      121 aggtcgggtc caccctgatt ccttcttcga agatggtctt tgcttccttc aatcgaccgg
      181 tcatgccata cgccattccc aagttgtcga ccagcacacg ccaaagggtc tggtcgagct
      241 gccgttgttg cttttcagat tcgtaggcct tctgatagaa gggaatggct tgatcatatt
      301 tactctgcaa gtaataggcg cttccggccc gaaagagttg catactgctc tgtctcgctg
      361 ccgattgtgc ctgcacgttc tggccgtctg catattgaaa ccgtacagtt tgaaggacgg
      421 actgaaacag ggactcgtcg cttggtgtgt atttcacctt cgacagatgg aggtcggcgt
      481 aaacattttc tttggcaaga cacgcacgca catttctttg atcaagtcga attccctgaa
      541 attctcgaaa cgtgtatgcg agggctggca tcttcgcaat cgtgacaaac ctcgcatcct
      601 gtcctttgga caccggaggt tgattgcgaa ggcgttgtaa atgcgctgag catccctctg
      661 ttgaggcttg gcctgcgacc ttttcaagtg tgaccgagac attaactcca gtcgtgggat
      721 gacttgccat caggtagcga cgaccatccg gtttgatttg atcgatgtcg attttaaaac
      781 ccgacaaatc gattgaaagg cctagtccct tctctggcca cgcgataact gtttgttgct
      841 gctcgctcgc gaacgccccg ttaatagata cggcgagaaa gaaagacgcg atgaagatag
      901 agactccgct gactcgtaaa atctcagatc tgctattctg tgatgtcatg gtttgcatct
      961 cccataacga ggtttgttct tcgtagatag agcagcacgg caattgggaa gagagccgtt
     1021 cggtctctgc tgatcagctg acacatgatc tatgatgtag gaagaggagt ggaagctcct
     1081 ctcttgctac aagctcctcg aacgaatttc cagtcgacat cggttagcag gcgtcgggtg
     1141 cctttagatg aatgaccagc gatggctcgt agacgagcga tgcgttgatt ccaatttgga
     1201 tggcttgaaa ggtaatctaa gactccgctt tcccgatcgt gcagttcccc aatgatgcga
     1261 taaaagttga tcatctcctc cggcatgagg ccagctttta taatgaggcg cataccttct
     1321 tcatccgcct ccgtctcgtg gccctgactg tattgaagtt gcccgacagc ctgagccgac
     1381 tgtaacccat aggtcattac cgagctaaaa tctccggcca gggtggaaag tagaactcct
     1441 gtcagaacct gctcggtgat catgcgggta gaatgccgct tcaagacatg ctggacttca
     1501 tgcgcgagga ctccggcaag ttgctctggt gaatctgttc gttcaagtag accacgatag
     1561 accacgatgt gtccgccagg agcagccaag gcattaacca ggggactatc cacaacatag
     1621 agatgaaact gatacggaga ctttggcgcg gcctcagtca gcgaagtcag aatattctga
     1681 atgagttgtg tgcgatgcgg atcggtacaa cgccgctctt gcggggctac gtatagaaca
     1741 gcttcctccc cgattcgttg ctcccaactg actggaacag tcgaggcaac aaccacagaa
     1801 agcatcggga taccccatcg gtacagcaca aacgcgaggc agatggcagt gaccgccgcc
     1861 acaaccgtcc accgcatgcg tttctggcgc tgcgagggat tatggatgtg ccagtgtttg
     1921 cgagtcgacc ggttgtgaag ttcggtcaag acgtctgaag atgaaatgac gagcgcttcg
     1981 agagtggata cacgttccag tctgacaggc tcaccagcgt aagatccctg cgtttgttga
     2041 atcgcctcga acggccaatg aaacacttgg ccggtattgg tggtgatgtt tagactgtcg
     2101 gccgagagtg ctattttcgc cggttggcga attggtgatt ttccatccag ataatacccg
     2161 gcccatgttg tgttagacat agaattcagt cgaactcgaa tccactgtcg agcaagttgg
     2221 cgagaccttc ggtcgtgact ggagcaacgt ctgattcctg aaagatcgat gcctcgtcca
     2281 gagagccgag gaggctcaga ttgttcaaat gaaattgaat cgtccgaact gtggtccatg
     2341 gccacgcaaa tcctaacgtt acgaccaaca atagcaagtt ccccgcatgg agatttagga
     2401 aggcgcgtgc cgttatggtc gaacgaaagc gggcttcccc caatttcgtg tggtcccaga
     2461 agaagcgttg ctttttggct aaaaaccaca gccagggtgg cgctacggta aagggcagca
     2521 ggaggagaaa gagcatgttg acgttgtagg caaagaccac gggaatgaaa aaggtaaaaa
     2581 acgtcactaa gggtgtcagt acgaaccccg gcgcgagttc ggcaccgatg ccatcgaatg
     2641 agaaagtccg gttgccgaat cggctatatg aaaccaggta cgcttggcgc ttcgtttgat
     2701 aatagggata gtatgcgccg agtgtgatcg ccgtcagagc ggttcccttt agcgtgagcc
     2761 agagaaaggg tcgaactttc ccatgaaagg aaaaccgaat cccgcgccat gacgttctgc
     2821 tcaagcgata ccgtcgggat cctatcattg caatgggagc aaagacccac ggcacgagga
     2881 cggccagcga actgcaggcc atcacagccc accaaggaag gtcgacaagt tgcggcacgg
     2941 tatttagcgc aacgtacgga aatccgaaaa gcgtagccgc cttaagaaag ccgacagata
     3001 gttctttgcc tgatccgtgg tacacgaagc ggtcgctggc aaattcggtt tgactaaaca
     3061 gatacttgcg caccttgacc tttgcccaaa agtgatagag accgagcgtg agcaaggtga
     3121 gagcgagatt gacaatgtgg atgccgaaca gcgtactcac cttgccgtgg aaggatgggc
     3181 acgtccgcgt ctcaggtagt gacatggggg cggcatgcgt ctctctcaag tcaagagagt
     3241 gcgtcatcag cctgacgccg aacgctgcac cgcagcgaga gcaatggctt gtggctttcg
     3301 gtttaagaac gcgcggatcc cgaacacggt gtggagtttg gca
//
LOCUS       DASTQY010000500         6804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_733431, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000500 DASTQY010000000
VERSION     DASTQY010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733431"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VFR82_16000"
     CDS             <1..904
                     /locus_tag="VFR82_16000"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEU4503153.1"
                     /translation="RLVLEDLARLKTALDRSKMCGCLAAYSAPYHYMWNALLPYWWMD
                     LDGALCLFIQSPDGWFMPLPPLTEGSLTLPLNKAFRLMRQWNGESAVSRVENVPAALA
                     PSLVAMGYRMAPKSPDYLYRADDLARLTGDRYKSQRALCNRVEREGAMVVELFHPRDR
                     AACRALLHEWRSQKLARGADPLAELLLEDSVSAHEVAWSHASELQLAGSVLRRNGTLC
                     GYTFGYWLDGQTWCVLLEVTDRTIPGSAQFLFRETCRQARSQGAEFINTMDDSGLTGL
                     RLSKEAYHPVACVESFVCSETSQP"
     gene            862..4395
                     /locus_tag="VFR82_16005"
     CDS             862..4395
                     /locus_tag="VFR82_16005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4503154.1"
                     /translation="MRREFRVLRDLAAMSKQDFQQPLDPPRYRWLPHLIILMNLVAIA
                     AGVFLLRSVEHRLVYSAGEELRIAAAEVADKLDRLLFERYGDAHMMAQALALRPSDTT
                     YLTSYVGWMKTNHAPVYAWLGVTDSRGMMIAATESSLRSQDFSQTGWFQRVSQTKTIQ
                     VDDVALHDGEGGTESVAFSAPILDAEGNLSGIVTSRVPISVLEEVTTGTLRSLEARPE
                     FAGAVVFRMVTDQGRVFVDSDLRRNRVSSQQDSGALSSRAAPFGDLGFVEEKFHGQAI
                     VTGFARTKGFGQFPGLGWHVLVRMDRDRILEPILSILWKVGLTGAVVWVPMVVLLFWS
                     TSHLRAEYRQVQQESSWARAAEAALLQSQERTRAIVETALDALVTTDAAGIVNDWNGQ
                     ASAIFGWSREEALGKRLTDLIIPQRDREHHERGIRQYLASGETHILNRRIEVLALRRS
                     GQEFPVELSISPVRVGDTIFFSAFIRDITERREADTKLKESEVRYRSVVNALDEGVVV
                     IDASGVLRTGNASAERILGVPCEELTGRMFHDKQWRTIREDGSPFPSEEYPAAITLKT
                     GEPCAAIVMGIYRPDGQLRWVEINSRPLTPEGGASPSAVVISFSDITGRKRVEERLTA
                     QYAVTRVLAESRSLEEAVPKIMRALGQNLEWDCGIFWRVDRTTSVLRCLDQWSGAGVE
                     AQSFLEKTWDLTFKQEEGLPGRIWSSGKAAWITDVARDSNFPRRLLASEVGFHGAFGF
                     PVRVGGDTEGVIELYSRQVRVPDNELLKMIDDVGLKLEQFGDRTRAQEALQQTEAQLR
                     QSQKMEAVGRLAGGVAHDFNNLLTVIRGYSELLLARLSADDPIRKDMTEIKKAADRAS
                     GLTRQLLAFSRRQFIAPKVIDLNALIANMDGMLRRLLGEDIVELCTELNPHTGWIRAD
                     AGQVEQVIMNLAVNARDAMPKGGRLTIETRNVTIGKPTRQTPAGVKPGAYVLLAVRDT
                     GHGMDEETQSHLFEPFFTTKEKGKGTGLGLSTVYGIVKQNGGNILVESQKDQGATFKI
                     YFPLVEQGAPGMVAPDAEPVHGRETILLVEDEPSVRGLVHETLRLHGYTVLEARHGIE
                     ALMTGAKHQGPIHLLLTDVVMPQMSGPEVAEKIQIVRPGIKVLYMSGYPDHPVFEQGG
                     ISRDASFLPKPFTPNVLAKKVRDVLDGGKVA"
     gene            4471..5289
                     /locus_tag="VFR82_16010"
     CDS             4471..5289
                     /locus_tag="VFR82_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEU4503155.1"
                     /translation="MTQQGREFDISQYRIEKEPYYADVRGEEGLFTTAAKNRMPVMLK
                     GPTGCGKTRFVQYMAYRLGRPLITVACHEDLTASDLVGRYLLKGQETVWVDGPLTLGV
                     KHGAIVYLDEVVEARKDTTVIIHPLSDDRRLLPIEKKGQAIEAAEDFLLVISYNPGYQ
                     NVLKDLKPSTKQRFVAIEFDYPAADIEASIVEREAGISHDLAARLVKLGGKVRNLKNH
                     GLEEGVSTRLLIYAGTLIREGIAIERACDVAIARPITDDADMQRSIMELVKAMF"
     gene            5418..5714
                     /locus_tag="VFR82_16015"
     CDS             5418..5714
                     /locus_tag="VFR82_16015"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEU4503156.1"
                     /translation="MKDWQGGVLLTIFVQPKASRTAWVGIHGDAVKIRIAASPVDGAA
                     NRELIRFLAEELSIPRTAIDIHSGAGGRRKRLCVKGTTAVHLAAHLIRKCQGKG"
     gene            5711..6136
                     /locus_tag="VFR82_16020"
     CDS             5711..6136
                     /locus_tag="VFR82_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HEU4503157.1"
                     /translation="MNLLRALVLLVGTLLSACSGAKPVLYPNAHLQSVGNETAEQDIE
                     ACKEAAEAAGAEEGSGKAGRVATSTGVGAGVGAASGAVGGAISGAAGQGSLIGAASGA
                     VWGLLTGLFTAGSSQPSQAFTNYVNRCLQERGYEVTGWQ"
     gene            6327..6686
                     /locus_tag="VFR82_16025"
     CDS             6327..6686
                     /locus_tag="VFR82_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503158.1"
                     /translation="MLRPNAKLLLKGREASNLEVAACQQKAEAAGLQPGTAGRGGNMA
                     AGAGLGLIAGAAVGASTGVIGGATGVAIGAAAGAGLGLIIGLAGGAYRPLEPDPPYAD
                     AVVRCLIEQGYEVTGWQ"
BASE COUNT         1414 a         1848 c         2157 g         1385 t
ORIGIN      
        1 acggctggtc cttgaggatc tcgcacgcct caagaccgcc ctcgaccgat ccaaaatgtg
       61 cgggtgcctt gccgcctatt ccgctccgta tcattatatg tggaacgcgt tgctgcccta
      121 ctggtggatg gacctggatg gggcgctatg cctctttatc caatcgcctg acggatggtt
      181 catgccgctg ccgccgctga ctgagggctc tctgacgctg ccgttgaaca aggccttccg
      241 tctcatgcgg cagtggaacg gggaaagcgc cgtaagccgg gtagaaaatg tcccggcggc
      301 gctcgcaccg agtctagtcg cgatggggta tcgcatggcg ccgaaaagtc ctgactatct
      361 gtatcgggcg gacgacctcg ccaggctgac aggggatcgg tacaaatctc agcgtgccct
      421 ctgcaatcgt gtcgagcggg aaggggctat ggttgtcgag ctctttcatc ctcgggatcg
      481 ggcagcttgc cgtgcgttgt tacacgaatg gcggagccaa aaactcgctc gaggcgcgga
      541 ccctttggct gaactgctgc ttgaggattc ggtctcagcc catgaagtcg cttggtccca
      601 tgcgtctgag ctgcaactcg cgggatcggt actgcgaagg aatggcacgc tgtgcggcta
      661 cacatttggc tattggctgg atggccaaac atggtgcgtg ttgttggaag tgaccgaccg
      721 gacaataccg ggatcggcgc aattcctgtt tcgcgagaca tgccgtcaag ccagatctca
      781 gggcgccgaa ttcatcaaca cgatggacga ttcggggttg accggattgc gtctctcgaa
      841 ggaagcctat catccggtcg catgcgtcga gagtttcgtg tgctccgaga cctcgcagcc
      901 atgagcaagc aggatttcca acaacccctc gaccccccgc gttatcgctg gctgcctcat
      961 ctcattatcc tgatgaacct ggtcgcgatc gctgccggtg tatttttgct tcgcagcgtc
     1021 gaacaccggt tggtgtactc cgccggggag gaattgagga tcgccgcagc ggaggtggcg
     1081 gataaactgg accgtctcct gttcgaacga tatggcgatg cgcacatgat ggctcaagcc
     1141 ttggcgttgc ggccatcgga caccacttat ctaacaagct acgtcggttg gatgaaaacg
     1201 aaccatgcgc cggtttatgc atggctcggg gtgacggata gtcgtggaat gatgatcgcc
     1261 gcgacggaat cttccctgcg cagtcaggat ttcagccaga caggctggtt ccaacgcgtc
     1321 agccaaacga agaccattca ggtggacgac gtcgctctgc atgacggcga agggggaacg
     1381 gagagcgtcg ccttctccgc gccgattctt gacgccgagg gaaatctttc cgggattgtc
     1441 accagtcgag tgccaatatc ggtactggaa gaggtgacga cggggacgct acgttcactg
     1501 gaagcgcggc cggagttcgc cggagccgtc gtttttcgaa tggtgactga ccaggggcgc
     1561 gtattcgttg attcggacct tcgacggaat cgggtctcca gccagcagga ttcgggcgcc
     1621 ttgtcttcca gggcggcacc atttggcgat ctcgggtttg tcgaagaaaa gttccatgga
     1681 caagctatcg tgacgggctt cgcccggacg aaagggtttg gtcagtttcc ggggttgggg
     1741 tggcatgtac tggtgcgcat ggatcgtgat cgcatcctcg agccgatcct ttccatatta
     1801 tggaaggtgg gtttgacggg cgcggtcgtg tgggttccca tggtcgttct tttgttctgg
     1861 tcgacctcac acctccgggc ggaatacaga caggtgcagc aggaaagctc ctgggcacgt
     1921 gcagcggaag cggcgctgct tcagagccag gagcgcacaa gagcgattgt ggaaacggcg
     1981 ctggacgcgc tggtcacgac cgacgcagcg ggtattgtga atgactggaa cgggcaggcc
     2041 tctgccatat tcggatggtc gcgcgaagag gcgttgggca aacgcctcac ggacctcatc
     2101 atcccccagc gcgaccgtga gcatcacgag cgcggcatcc gccaatacct tgcgagcgga
     2161 gaaacgcaca tcctcaaccg ccggattgag gtgctggcgc ttcgccgctc cggacaggaa
     2221 tttcctgttg agctttcgat ctcaccggtt cgggtgggag acacgatatt tttcagcgcc
     2281 ttcattcgtg atatcaccga gcgccgggag gcggacacga aactgaagga gagcgaagtc
     2341 cggtatcgat cggtggtgaa cgcgctcgat gaaggcgtcg tggtcatcga tgcctcaggg
     2401 gttttacgca ccggcaacgc cagcgccgag cggatcctcg gcgtaccttg cgaggagcta
     2461 accggccgta tgtttcacga taagcagtgg agaaccattc gtgaagacgg gtcgccgttc
     2521 ccatccgagg aatatccggc ggccatcaca ctaaaaacgg gggagccctg cgccgccatc
     2581 gtcatgggaa tctaccgacc ggatggacag ttgcgttggg tcgagatcaa ctcccgaccc
     2641 ttgacccccg aaggcggagc cagcccatcc gccgtcgtga tctcgttttc ggatattacc
     2701 ggacgcaaac gtgtggagga gcggctcacc gctcagtatg cggtcacgcg cgtgctggca
     2761 gaatcgcgga gcttggaaga agccgttccg aagatcatgc gggccctcgg ccaaaatctc
     2821 gagtgggatt gcggaatttt ctggcgcgtg gatcgcacca catcagtgct ccgttgtctg
     2881 gatcaatggt ccggggccgg ggttgaggcg cagtcgtttc tcgagaagac ctgggacctg
     2941 accttcaagc aggaagaagg cttgcctggt cggatctggt cgagcggcaa ggcggcgtgg
     3001 attaccgatg tggctcgcga ttccaatttc ccgcgccgac tgctcgccag cgaggtcgga
     3061 ttccacggcg cgttcggatt tccggtgcga gtgggcggcg acaccgaagg cgtcattgaa
     3121 ctgtacagtc ggcaggtccg cgtgccggat aacgagctgc tcaagatgat cgacgacgtc
     3181 ggcttaaaac tggaacagtt cggtgatcgg acgcgggccc aagaagccct ccaacagacg
     3241 gaagcacagt tgcgccaatc ccaaaaaatg gaggcggtag gacgcctcgc gggcggggtc
     3301 gcacacgatt tcaacaatct tttgaccgtc atccgcgggt atagcgaatt gctgctcgca
     3361 cgtctgtcgg ccgatgatcc aattcgcaag gatatgactg agatcaaaaa agcggctgat
     3421 agggcttccg ggctgacccg tcaactgctg gccttcagcc gccgtcagtt tatcgcgccg
     3481 aaagtcatcg atctcaatgc tctcattgca aacatggacg gcatgttgcg cagactgttg
     3541 ggtgaggaca ttgtcgagct ctgcacggaa ctcaatcctc acaccggatg gatccgagct
     3601 gatgccggac aggttgagca agtgattatg aaccttgccg tcaatgcgcg cgatgccatg
     3661 ccaaaagggg gtcgtctcac gattgagacc cgaaacgtga cgatcggcaa accgacccgt
     3721 cagacaccgg ccggtgtgaa gccaggagcc tatgtgctgt tggcggtgcg cgataccggt
     3781 cacggaatgg acgaagaaac gcagtcgcat ctgtttgagc cgtttttcac gaccaaggag
     3841 aaaggaaaag gcaccggcct cggtctttca actgtgtatg gaatcgtgaa acagaatggc
     3901 ggaaacatct tagtcgaaag ccaaaaagac caaggcgcga ccttcaagat ttattttccc
     3961 ctcgttgagc agggtgcgcc gggaatggtc gcgcctgacg ccgaacctgt gcatggccgc
     4021 gaaaccatcc tgctggtgga agacgagccg tcggttcgtg ggttggttca cgagacgctg
     4081 cgactccatg gctacaccgt gctcgaagcc cgtcatggca tcgaggcgct catgaccggt
     4141 gccaagcatc aaggtccgat tcatctcctg ctcacggatg tcgtcatgcc gcaaatgagc
     4201 ggcccggaag tcgccgaaaa gatccaaatc gtgcggcccg gcatcaaggt gctgtacatg
     4261 tctggctatc cggatcatcc ggtatttgag caagggggca tctcccgcga cgcgtcattc
     4321 ctgcccaagc cattcacccc gaatgtgttg gcaaagaaag ttcgtgacgt gttggacggc
     4381 ggaaaagttg cataacggag ccccttgctt gatcttctca ctgggttccg tttatcgtgc
     4441 ggtgcaattc gtcgattcga gaaagcttct atgacgcagc aaggacgtga gttcgacatt
     4501 tcgcagtatc gcatcgagaa ggagccctat tacgccgacg tgcggggtga agagggtctc
     4561 tttaccactg cggcaaagaa ccggatgccg gtgatgctca agggaccgac cgggtgcggg
     4621 aaaacgcgct tcgtgcaata catggcgtat cgcctcggcc ggcccctgat cactgttgcc
     4681 tgccacgaag acttgacggc ttcggacctc gtgggtcggt atttgctcaa aggtcaggag
     4741 acggtctggg tggacggacc gctgacattg ggtgtgaagc acggtgcgat tgtctacctc
     4801 gatgaggtgg tcgaagcgcg aaaggatacg acggtcatta ttcatccgct cagcgacgat
     4861 cgccgactgc tgccgatcga gaaaaagggc caggccatcg aagccgctga agatttcctc
     4921 ctcgtcattt cctacaatcc cggctatcag aatgtgctca aagatttgaa gcccagtacc
     4981 aaacaacgat ttgtcgcgat cgagttcgac taccccgctg ccgacatcga agcctcaatt
     5041 gtagagcgtg aggccggcat ctctcacgac ctcgctgcca gactcgtcaa gttgggcggg
     5101 aaagtgcgca atttaaagaa tcacggcctc gaagaaggcg tgagcacgcg acttctcatt
     5161 tacgccggga cgctcattcg agaaggtatc gcaatcgagc gtgcctgcga cgtggcgatt
     5221 gccagaccga tcactgacga tgccgacatg cagcgcagca tcatggaact ggtcaaggca
     5281 atgttctgag cagtatgcga atctgtggct cgcgctgtgc aaaagactca actgccgcca
     5341 caaagggaat gcggaaccga cggtccgcag ccttctagcc agtgccggac ctctcctcat
     5401 cggtcgtgca ctcatttgtg aaagactggc agggtggtgt cctgcttacg atctttgttc
     5461 agcccaaggc ttcccggacg gcctgggttg gtatacacgg cgatgcagtc aaaattcgca
     5521 tcgcggcatc tcccgttgac ggagcagcaa accgtgaact gatccgtttc ctcgccgagg
     5581 agttgtcgat accgaggacc gctatcgaca ttcactccgg cgccggtgga aggcgcaaac
     5641 gcctctgtgt aaaaggaacg acagcggttc acctcgcggc gcatctgata aggaaatgtc
     5701 agggaaaagg atgaacctgc tcagagcctt ggtgctgttg gtcgggacgt tgctgagcgc
     5761 ctgttcagga gcgaaaccgg tcttgtatcc gaacgcgcac ttgcaatcgg tggggaatga
     5821 gacggcggag caggatatcg aagcatgtaa agaagcggct gaagccgccg gcgcggaaga
     5881 aggcagcggc aaggcgggtc gtgtcgccac aagtacgggg gttggcgccg gggttggggc
     5941 agcgagtgga gcggtcggag gcgcgatttc cggagcggcc ggtcaaggat ccctgattgg
     6001 ggcagccagt ggagcggtgt ggggattatt gacgggcctc ttcactgccg gcagctctca
     6061 accgagccag gccttcacga attatgtgaa tcggtgtctg caggaacggg ggtatgaggt
     6121 gactggttgg caataatggc gagaagggcg ggccacgcat gtgagaccgt cacgagtgac
     6181 gtgtccaaga agtcgtcctc ctatcagaaa gatgagagga tgcagaaccg gtgaacaggc
     6241 ggaacgagcc gggatccggt agatgggagt ccattctcat actatggctt gtttttgcgc
     6301 ttgccggctg ctcgggacct gagccgatgc tgcggccgaa tgccaagttg ctgttgaaag
     6361 gccgcgaagc ttccaatctg gaggtcgccg cgtgccagca gaaggcggaa gcagccggcc
     6421 tacagcccgg gactgccggg cgaggcggaa acatggctgc gggcgcggga ctgggactga
     6481 tcgccggtgc agcggtcggg gcgtcgaccg gagtgatcgg aggtgcaacg ggtgtggcca
     6541 taggcgctgc cgcgggcgcc ggccttgggc tcatcattgg attggcaggc ggggcctacc
     6601 gaccgctaga accggatccg ccttatgctg atgccgtggt gcgatgtttg atcgagcagg
     6661 ggtatgaagt cacagggtgg caataactca atccgctgtt ttttccggtg aggcgttcaa
     6721 agccttatgc cgtagcgccc gacccttgtc gggcgtggga tcggtaatca caccatacac
     6781 ttcccgtcct tcgtgacctt ccgg
//
LOCUS       DASTQY010000501         4643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_336661, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000501 DASTQY010000000
VERSION     DASTQY010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336661"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFR82_16030"
     CDS             complement(<1..317)
                     /locus_tag="VFR82_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEU4503159.1"
                     /translation="MPDAVGTTPFAYRTFTLFCLVVAVTLIGTPLFGYLYGYTLFDWA
                     LFAILYVCTGLGITVGYHRLISHRSFECRPWVKALLLIAGGWALQNSALRWSADHLRH
                     HART"
     gene            605..3547
                     /locus_tag="VFR82_16035"
     CDS             605..3547
                     /locus_tag="VFR82_16035"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEU4503160.1"
                     /translation="MAAINPLESSVQCIGGELARLSAGQAPALFERQWWSQAAINLAM
                     HDLDFKTQLFRFIDVLPSVADDQRVVSLAQEYFGAMADQAFGLRWGLKALSATSIGAR
                     LSGRAIRAQVEQMARTFIAGSSIEDAAPVLGALWTGGKAWSVDLLGEATISDQEADRY
                     RDRCVAALEALAQPAGAWPAAPLLEKDHLGAVPRVQLSLKISALSPFLDPIDPEGSFK
                     SVAARLRPIVDAAMRSPASLIFDMEQADTKSLIIEIFTRLFSETPYRQFPHAGLALQA
                     YHRETTQDVASLLGWVRTRGAPITIRLVKGAYWDSDTIRYRQRGWPLPLFEEKADTDF
                     NYESLVRILLEASSLIRPAFGTHNLRTLAYVEAVAEQVGLPPSTWEYQMIFGMAEPFQ
                     QAVLRRGRRLRLYTPVGDLLPGMAYLVRRLLENTSNESFLRKEYVESQPLTTLLARST
                     AQTAPMPASEPTGFRNEPLRDFSQPEARSAMQEGIGMVKGQLGRLWPPVAGLVTLSGP
                     LLASHNPSRPDEVVGVVQAADGSDVEQAVRQAGDAWPAWRDMPATERVEVLQAAASLM
                     RARRETLAAWEILECGKSWREADADIAEAIDFLEFYAAEGLRLSTPKRLGHESGELNH
                     RVLSPRGITAVIAPWNFPLAIPTGMVSAALVTGNPVIFKPSERSPLVGRILVQMLVEA
                     GVPEAIVSCLPGGPTVGQALVRHPAVVTIAFTGSKQVGLKLLKDTSTIVTGQRMVKRV
                     IAEMGGKNAIIVDETADLDDAVAGVVASSTSYSGQKCSACSRVIVHAAVYDLFLSRLR
                     DAITSLRVGNPEEAGTQMGPVIDERAQASILEYIKIGSQEARVVVEGRVSQPGWYVAP
                     TVLADVKPTARIAQEEIFGPVLAVMRAESFDEALTVANGTDYALTGGVYSRSPANLEL
                     ARRRFDVGNLYLNRPITGALVNRQPFGGHRLSGVGAKAGGEDYLLQFVVTRIVSEQTL
                     RRGFAPAE"
     gene            complement(3440..4303)
                     /locus_tag="VFR82_16040"
     CDS             complement(3440..4303)
                     /locus_tag="VFR82_16040"
                     /inference="COORDINATES: protein motif:HMM:NF045041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503161.1"
                     /translation="MRFWSLTVLYLVLPWSVSLAAEELNGILQSSDYFPDTIGSHWEY
                     HGQITEGPLQTIEHKFFTNVSTVVGTKPLRGMTVTVFHDTNPGNHGPSDSFYRRDVVG
                     IVYHGSDPGTPLEKQLIPYQIVRFPIQIASTIQQFDKKGLDFGTDMDRDGVNEKVDAH
                     GTSTVAGLESITVPAGTFKDAVKVEAYMRMKIHLSGASRTVTGTDVMTAWFAKGVGLI
                     KYVERQELSPLEDRGVITEIMEELDSYEVKSPKASLGRSESPAERLFAHDSRDHELQQ
                     VVFPSSFRPHP"
     gene            4454..>4643
                     /locus_tag="VFR82_16045"
     CDS             4454..>4643
                     /locus_tag="VFR82_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4503162.1"
                     /translation="MNSRPSAESSRQARAGSRAVLVTGASGGIGQAISCGFGKAGWCV
                     GIHYHRQKHAAESTLHDVQ"
BASE COUNT          938 a         1318 c         1410 g          977 t
ORIGIN      
        1 gtcctcgcgt gatggcgaag atgatcggca ctccagcgga gggccgaatt ttgcagtgcc
       61 catccgccgg cgattaacag cagcgctttc acccagggac ggcactcgaa gctgcgatgt
      121 gaaatcaatc gatgatagcc gaccgtaatg cccaggcccg tacacacgta gaggatggcg
      181 aagagagccc agtcaaacag cgtatagcca tacaggtaac cgaaaagtgg cgttccgatc
      241 agcgtcacgg ccacgacgag acagaagagc gtaaacgtcc tataggcaaa cggggtggtt
      301 ccaacagcgt ccggcattct cactccttct gagtagtaaa cagcagttga tgaagcgtta
      361 tcgctggctc gggcacgttg ctttgcggca cgcagagctt ctcagcccac tcaaagagcc
      421 gtactcactc cggcgtgtta gggattcacg atcgctctgg gccgggactg aagtctcgct
      481 gagtaagagt accgaagtag cagactttaa tcaagaaatt ctttgccctc acgcgaggcg
      541 aactcaagac cgaggtgtat actgaatgtt gagtcagggt tatccgtaag ctggtaggtc
      601 cgtcgtggct gccattaatc ctctggaatc ttctgtccaa tgcatcggcg gagagctcgc
      661 ccgcctctct gcagggcagg ctcctgctct ctttgaacgt caatggtggt cgcaagcggc
      721 gatcaacctg gcgatgcatg atctcgactt taagacacag ctgttccgct tcattgacgt
      781 actgccgtcg gtcgccgacg atcaacgggt cgtgtccttg gcacaggaat acttcggggc
      841 gatggccgat caagcctttg gtcttcgctg ggggctgaag gccctatctg ctacgagcat
      901 tggcgcccgt ctgagcggac gggccattcg tgcccaggtg gagcagatgg cgaggacctt
      961 tattgccggc tcgtcaatcg aggatgccgc gccggtcctg ggcgcgttgt ggaccggcgg
     1021 caaggcctgg tccgtcgatc tgctcggcga agccacgatc agcgaccagg aagccgatcg
     1081 ctaccgggat cgatgtgtgg ctgccctgga ggcgttggct cagccagcgg gagcctggcc
     1141 ggccgctccc ctcttggaga aagatcatct cggtgccgtg ccgcgggtgc agctctcact
     1201 taaaatttct gcactttctc cctttctcga tccgattgat cccgagggca gctttaagtc
     1261 ggtcgcggca cgcctgcggc ccatcgtgga tgcagccatg cgatcgccgg cctcgctgat
     1321 cttcgacatg gaacaagccg acacgaaatc gctcattatc gagatattca cccggctgtt
     1381 ctcggaaaca ccctatcgcc agtttcccca tgcgggattg gccctccagg cttaccatcg
     1441 agaaaccact caggatgtgg catccctgct tggatgggtt cgcacacgcg gtgcgccgat
     1501 cacgatcagg ctggtgaagg gcgcctactg ggactcggac acgattcgtt accgccagcg
     1561 gggttggccg ctgccgctat tcgaagagaa agcggacacc gacttcaact acgaatcatt
     1621 ggtgcgtatc ttgttggagg catcttccct catccgtccg gcgttcggca cgcacaacct
     1681 gcgcacactc gcttatgtcg aggccgtggc tgaacaggtg gggcttcccc cctccacgtg
     1741 ggagtaccag atgatcttcg gcatggccga gcctttccag caggccgtgt tacggcgagg
     1801 ccgtcgtctg cggctctata cgccggtcgg tgatctgctc cccggcatgg cctatctcgt
     1861 ccgacggctc ttggagaata cgtcaaatga atcattcttg cgaaaggaat atgtggagtc
     1921 gcagccgttg acaacgctct tggcgcggtc gacggctcag acggctccga tgccggctag
     1981 cgagccgacc ggctttcgca atgaaccatt gcgcgatttt tctcagcctg aggcgcgatc
     2041 agccatgcaa gagggtatcg gcatggtgaa aggccagttg ggcaggttgt ggccaccggt
     2101 tgccggtctg gtgacgctta gcgggcctct cctggcctca cacaatccga gtcgcccgga
     2161 cgaggtcgtt ggtgtcgtcc aagcagccga cgggagcgat gtcgagcaag ccgtccgtca
     2221 ggcaggcgat gcgtggccgg cttggcgtga catgccagct acggaacggg tggaggttct
     2281 gcaagccgcg gcatccctca tgcgggctcg tcgagagact ctggcggcct gggaaatcct
     2341 ggaatgtgga aaatcctggc gcgaggcgga tgccgacatc gctgaggcga tcgatttttt
     2401 ggaattctat gccgcggagg ggctccgact ttcgacgccc aagcgcttag gacatgaatc
     2461 aggtgaactc aatcatcgcg tattgagtcc acgtgggatc accgcggtca ttgcgccctg
     2521 gaattttcct ctcgcgattc cgaccggcat ggtgtccgcg gcgcttgtga cgggtaaccc
     2581 cgttattttt aaaccctcag agcgctcgcc cttggtcggc cgcattctcg tgcagatgct
     2641 ggtcgaagcg ggggtgcctg aggccatcgt ctcttgtctc cccggaggac cgaccgtggg
     2701 acaagccctg gtgcgtcatc ctgcggtggt caccattgcc tttacgggct cgaagcaagt
     2761 cgggctcaag ctcctgaagg acacatcgac gatcgtgact ggccaacgga tggtgaaacg
     2821 agtgattgcc gaaatgggcg gcaagaatgc gatcattgtc gacgaaacgg ctgacttgga
     2881 tgatgccgtt gccggtgtgg tggcgtccag cacgtcctat agtgggcaga agtgctctgc
     2941 ctgttcacgc gtcattgtcc atgctgcggt gtatgacctc ttcctgagcc ggcttcggga
     3001 tgcgatcacg agcctccggg tgggcaaccc tgaagaagcc gggacccaga tgggaccggt
     3061 catcgacgag cgggcgcagg ccagcatcct ggagtacatt aaaatcggca gtcaggaggc
     3121 gcgtgtggtc gtcgaaggcc gtgtgtcgca gccaggctgg tacgtcgcgc cgaccgtcct
     3181 cgccgacgtc aaacccaccg ctcgcatcgc acaagaggaa atcttcgggc cggtcctggc
     3241 ggtgatgaga gcagaatcgt tcgacgaagc gctcacagtc gcaaatggga cggactatgc
     3301 gctgaccgga ggagtctact cgcgtagtcc cgccaacctc gagttggcgc ggcgccgatt
     3361 cgatgtcgga aatctctatt tgaaccgacc gatcaccggc gcacttgtca accgccaacc
     3421 atttggcggc caccggctgt caggggtggg ggcgaaagct ggaggggaag actacctgtt
     3481 gcaattcgtg gtcacgagaa tcgtgagcga acagacgctc cgcaggggat tcgctccggc
     3541 cgagtgatgc cttcggcgat tttacttcgt atgaatccaa ctcctccatg atttccgtaa
     3601 tcactcctcg gtcttcaagc ggagacagtt cctgtcgctc cacatatttg atcagtccaa
     3661 cccctttggc aaaccaggcg gtcatcacat ccgtgccggt gacggttcgt gaggcgccgg
     3721 agagatggat tttcattcgc atataggctt caaccttgac ggcgtccttg aaggtcccag
     3781 ccggtactgt gatcgattcc aacccggcga cggtcgaggt gccatgggcg tccactttct
     3841 cattcacgcc gtcccgatcc atgtccgttc cgaagtcgag ccccttctta tcaaattgtt
     3901 gaatggtcga ggcaatttgt atcgggaagc ggacgatctg atagggaatg agctgtttct
     3961 caagcggagt gccgggatca gatccatgat acacaatgcc gaccacatca cgccgataga
     4021 agctgtcgga cggcccatga ttaccggggt tggtatcatg aaacacggtg acggtcatcc
     4081 ctcgaagtgg tttggttccg actaccgtgg acacgttggt aaaaaatttg tgttcgatcg
     4141 tctgcagcgg cccttccgtg atttgaccgt gatattccca atgacttccg atggtatctg
     4201 gaaaatagtc cgatgattgc aaaatgccat tgagctcttc ggcggcaagc gaaaccgacc
     4261 aagggagcac caggtacaag actgtcaggc tccagaatcg cacgtagtgg ctcaagcgtg
     4321 tgggcataga ggcaataagc taaaggttaa tgaaacggta tcacatgaat cgtgagggca
     4381 gcaagggcgg agaaacttca taggctcggg cggacttgcc ttcgtgcctt gagcacacca
     4441 taatcccttg cccatgaaca gtcgtccaag cgcagaatcg agccgtcagg cgagagccgg
     4501 gtcgcgcgcc gtcctcgtga ctggcgcttc cggcgggata ggccaagcca tcagctgcgg
     4561 gtttggaaag gccggctggt gtgtgggcat ccactatcac cggcaaaagc atgcggccga
     4621 gtcgacgctg cacgacgtgc aag
//
LOCUS       DASTQY010000502         8999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_648702, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000502 DASTQY010000000
VERSION     DASTQY010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 8999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8999
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648702"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1999
                     /gene="bamA"
                     /locus_tag="VFR82_16050"
     CDS             <1..1999
                     /gene="bamA"
                     /locus_tag="VFR82_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEU4503163.1"
                     /translation="EKPFITEIVFDGNEELSDDKLKEKITIKSQAFLDQQQAKESAEK
                     IRLAYQEDGYFSAQVIPIVQTLDADRKRLTFFIKEGEKAKVKTVNFEGMRAASKAEIF
                     KVTATREWVPWYGLVTQLKLPSFLSDAGVLKREEMNNDVERIREVLLNKGYLNAQVGL
                     PSVELSDDKKWFVVTYAVVEGEPFTVAEIGFRGNTVFEDPELRDKMKIKPGEIFQRQK
                     IRDEIGRLTDLYGSKGYAFAEVIPNVNPNPEERTATIILNIKEGEMMRIRQINVHGND
                     KTKDNVIRRELRVDEQDVIDTPSLKRSFQRLNNLNFFETVEILPQQVDADKVDLNVRV
                     KEKPTGQFSIGGGFSTLDKLVAIADITEGNLGGNGWMGRIRGQLGQQRTLGLITFRNP
                     YLNDSLTSLQLDIYRSMTNYITYFEEKSGASITFGRWLSEYVTGSISFVAEELNFSDP
                     TIDAPEIILSQLGKQTTTGFRTTLARDTRDYFLDPRSGLRTAVGFDFGTPYLGGTNNF
                     YKYYLDAIKYTPLPFDTRFSIHARYGVADGIDGKPIPLTERFFVGGINTMRGFVFGRA
                     GPVTPSGSLIGAAKELIFNNDFIFTISSEAKLNGVIFFDWGKGFDDNEAISFGALRKA
                     AGLEGRWISPFGPLRAAYGINLDPREGERKGVFEFTIGSLF"
     gene            2030..2587
                     /locus_tag="VFR82_16055"
     CDS             2030..2587
                     /locus_tag="VFR82_16055"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEU4503164.1"
                     /translation="MRIEWPWVLARRLWVFSLAMWIATAFGCAGAGPKIDGKIGLVDP
                     GRVLNDSNAGKKAKESLTAFSKNRQALIEIEEKELRRMEEDFIKQASVLSPAAKNERE
                     QVFRRRMAEYQQKAAELNREVQEKQKDVLEAFRDKVEAVVAKVAKRNGLQVVIDKGKG
                     GPAIFGAEELDLTNQVIEEFNREYP"
     gene            2622..3158
                     /locus_tag="VFR82_16060"
     CDS             2622..3158
                     /locus_tag="VFR82_16060"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEU4503165.1"
                     /translation="MKRNTRVFLASIGSMWWMVSSMALAADPVKVGVMDQQLVIEKSK
                     AGKRALEELKAYSATRQKIINSDDQELKELEQTIQDAKLSEASKQEKQNQFQAKLEAY
                     QRRLAEFNREIQQKQREMVTEYSKKVQDAAQVVAQREGYIAVIDRGNEAAIRIVIYHQ
                     PALDVTEHVVKEFDKQNK"
     gene            3406..3873
                     /gene="fabZ"
                     /locus_tag="VFR82_16065"
     CDS             3406..3873
                     /gene="fabZ"
                     /locus_tag="VFR82_16065"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU4503166.1"
                     /translation="MTVMEQVEIQALLPHRYPFLLVDRILELEPDRRIVGIKNVTVNE
                     PFFQGHFPGRPVMPGVLIVEAMAQVGGLLAFKSLGPVAKPLVYLTGIDGAKFRKPVVP
                     GDRLRIEIDVLKKRSPFWKMLGKAFVESEVVCEAEVTAMVSEEKSASGGPSAM"
     gene            3938..4744
                     /gene="lpxA"
                     /locus_tag="VFR82_16070"
     CDS             3938..4744
                     /gene="lpxA"
                     /locus_tag="VFR82_16070"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEU4503167.1"
                     /translation="MEIHPQAIVHPKATLADDVSIGPFCVVGEHVTIGQGTRLLSHVN
                     VEGWTKIGARCELHPFVSMGGPPQHLGYQGEPTQVVVGDDNIFREYVTVNRATVQGGG
                     ITSIGDKNFLMAYCHVAHDCRLGSHLIMANAASLAGHIAISDHAIIGGLTGVHQYVRI
                     GAYAMVGGCCAVTQDVPPFMRVAGGYPARMYGLNSVGLRRHGFSAERIAVLKRAYDIL
                     FRSGHRMAEAAKLAKREFGDQPDVMAVLAFLEGTKRGICRSVGKDQADEE"
     gene            4748..5611
                     /gene="lpxI"
                     /locus_tag="VFR82_16075"
     CDS             4748..5611
                     /gene="lpxI"
                     /locus_tag="VFR82_16075"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249556.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEU4503168.1"
                     /translation="MTQAPLVGGGERLGLIAGNGRFPIIFADNARKLGYHVSAVAHEG
                     ETDPELANHVDRIHWVKIGQLNKLIHAFKEDGVHHAVMLGGIKKTHVFTTVRPDFRTL
                     ALATRLALWKDDDILREFAKELEGEGITICESTFGLEGILAEEGTLTSRKPTEKEWED
                     IRYGWDVAQEIGRLDIGQCVVVKDRMVVAVEAVEGTDEAIKRGGQLAKGGAVVVKRCK
                     PQQDLRFDLPAVGPRTIDVMASVSAAVLAVEAGRTILLDREVTLQKAQSNRIAVVGLV
                     KTDQSGGSEAR"
     gene            5545..6564
                     /locus_tag="VFR82_16080"
     CDS             5545..6564
                     /locus_tag="VFR82_16080"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU4503169.1"
                     /translation="MKSDSRSRVGQNRPERRVGGAVTPLRAGVIGVGHLGQHHARLYA
                     SLPGSRLVAVNDQSIERAHTIADRHEARVCKSLDELLSLVDVVSVAVPTSSHYAVAKA
                     CLLAGKHVLVEKPIAVTMAEAQELVRLAEDKHCRLQVGHSERFNPVMQVMRPHIGRPV
                     FIECHRLSSFSERGTDVDVILDLMIHDLDMVLSFSPGPVEDVQAAGVPVLSSTIDIAN
                     ARIQFKSGCVANLTASRVSTNKMRRLRLFQRDNYVSIDFQTRQGMICRREAGSGLKPT
                     IDFEQLQGGEEEPLKLQLESFLTAAVTGARPTVSGEDGAAALDLAHQVLKAIDVFVRR
                     NQEKG"
     gene            6595..7725
                     /gene="lpxB"
                     /locus_tag="VFR82_16085"
     CDS             6595..7725
                     /gene="lpxB"
                     /locus_tag="VFR82_16085"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEU4503170.1"
                     /translation="MPRILIVTGEASGDLHGAHLAAAIVAQDPTAEVIGIGGAAMRAA
                     GVTLVPDVPQLDVMGLIGLSAVRTLMGRLSAIRRILRSEPWDLVVLIDNPGLNLHFAR
                     VAKAAGRRVLYYIAPQVWAWRPGRMKWIQRRVDHVVVILPFEARLYERAGVRCTFVGH
                     PLLDSVAPHYDKTTLRRRYQLPESAPVVGLLPGSRAAEVTMLLPLLLETAAQAAAREP
                     NTQFLLAQAASIDDNLLHRLLEDSPVRVQIVKDQASEVMAASDLLVVASGTATLQAAV
                     IGTPMILVYKTTLPTYWLARLLIRVKWIGLVNLVAGRTIVPELIQDEATAERLTREVG
                     HLLSDQQAYNRMKDGLREVRQALGEPGASRRAARVVLQECRA"
     gene            7713..>8999
                     /locus_tag="VFR82_16090"
     CDS             7713..>8999
                     /locus_tag="VFR82_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEU4503171.1"
                     /translation="MSSVDRFIRLVRYLKPYRLRLIAAFICSALVAAFSGAYAWLVKP
                     VLDEIFINKNESLLLVLPLALFAVAVLKSAFSYGQNYLMNYVGNQVVTDIRQELFGRM
                     IRLPVKYHDSNTSGRLVSRVINDVTLMANAVAGVLKDVFQQGLTFVVMLGVIFYQNWK
                     LGGMSVIVIPLTVVTMMRMGKRLRSLGKSGQEQMGDMASTLQETLAGIRMVKAYGREA
                     AEAERFKESNKAFFGTTMKAIQVSSLGSSHIEVIGVVGVAVIIWYGGSLVIHGEMTPG
                     AFFSFLAAMFMVFTPLRRLSGSNNTIQQALAAAERVFGVIDLETEESTDRGRLVMQPI
                     ARSVVYQDVTFLYEGQAIPALTDIDLVIQAGEMVAIVGSSGSGKTTLANLLPRFYDPT
                     AGRVLIDGVDIQSFTLASLRAQIGIVSQDVVLFDDTV"
BASE COUNT         1991 a         2375 c         2791 g         1842 t
ORIGIN      
        1 agagaaaccc ttcattaccg aaatcgtctt cgatgggaat gaggaattaa gcgacgacaa
       61 gctgaaggaa aagatcacga tcaaaagcca ggcatttttg gatcagcagc aggcgaagga
      121 gagcgcggaa aaaattcgcc ttgcctatca agaagacggc tatttcagcg cccaagtcat
      181 cccgatcgtc cagaccttgg atgccgatcg taagcggctc acctttttca tcaaggaagg
      241 cgagaaagcc aaggtcaaga ccgtaaactt cgaggggatg cgggccgcgt ccaaggccga
      301 gatattcaag gtgaccgcga ctcgggagtg ggtaccctgg tatggcctgg tgacgcagct
      361 caagctgccc tcctttctct ccgatgccgg cgtcctgaag cgcgaggaaa tgaacaatga
      421 cgtcgagcgg atcagggagg tgttgctcaa caaagggtat ctgaacgccc aggttggctt
      481 accctcagtg gagttgagcg acgataaaaa atggttcgtc gtcacctatg ccgtcgtcga
      541 aggagagccg ttcaccgtgg ccgagatcgg gttccgcggc aatacggttt tcgaagatcc
      601 ggagttacgg gacaaaatga agatcaagcc aggagaaatc tttcagcggc agaaaatccg
      661 cgatgaaatc ggccgactga ccgatctcta cggcagcaaa ggctacgcct tcgcggaggt
      721 cattcccaac gtgaatccga atcccgaaga acgcaccgcg accatcattc tcaatatcaa
      781 ggaaggcgag atgatgcgca tccggcagat caatgttcat ggcaacgaca agaccaaaga
      841 caacgtcatc cgacgcgagt tgcgcgtcga cgaacaggac gtcatcgaca cgccgtcgct
      901 caagcgaagc tttcagcgcc tgaataatct caacttcttc gaaacggtcg aaatcctgcc
      961 gcagcaggtg gacgcggaca aggtggactt gaacgtcagg gtaaaagaaa aaccgacagg
     1021 gcagttcagt atcggcggcg gattcagtac tttggacaaa ctggtggcca tcgccgatat
     1081 cactgaagga aatctcgggg gcaacggatg gatgggccgg attcgtggac agctcggcca
     1141 gcagcggacc ttgggcttga tcacgtttcg aaatccgtac ttgaacgatt cgctgacctc
     1201 cctgcagttg gacatctacc gcagcatgac caactacatc acgtacttcg aagagaaatc
     1261 gggcgcgagc attaccttcg gccggtggct gtccgaatat gtgacgggaa gcatcagctt
     1321 cgtcgcggaa gagctgaatt tcagcgatcc gacgatcgat gcgcctgaga tcattctgag
     1381 ccagttgggc aagcaaacca cgaccggatt ccgcacgacg ctggcgcgcg atacacggga
     1441 ttattttctg gacccgcgaa gcggtctgcg gactgccgtg ggtttcgatt tcgggacgcc
     1501 ctatttggga ggcacgaaca acttctataa atattacctt gatgccatca aatatacgcc
     1561 gctgccgttc gatacacgat tctctatcca tgcccgctat ggtgtagccg acggaataga
     1621 tgggaaaccc attccgttga cagaacggtt ttttgtcggc ggcatcaata cgatgcgcgg
     1681 attcgtcttc ggtcgggcgg gcccggtgac gcccagtggt tcgctgatcg gtgccgccaa
     1741 ggagctcatt ttcaacaacg atttcatttt cacgatttcc tccgaggcaa aattgaacgg
     1801 tgtgattttt ttcgactggg ggaaagggtt tgatgacaac gaagcgattt cctttggtgc
     1861 attgcgcaaa gcggccggtc tggagggacg gtggatctcc ccgttcggcc cgctgcgcgc
     1921 tgcatacggt atcaatctgg atcctcgcga aggcgagcgg aaaggcgtgt ttgagttcac
     1981 gatcgggtcg ttgttctaac acgtgtcgag gtgaaaggtg agcccaggag tgcgcattga
     2041 gtggccatgg gtattggcac gcaggctgtg ggtattctca ctggccatgt ggatcgccac
     2101 agccttcggc tgtgcggggg ccggtcccaa gatcgacgga aaaatcggac tcgtcgatcc
     2161 gggacgcgtg ctcaacgact cgaatgccgg gaaaaaggcg aaagaatcgc tgaccgcgtt
     2221 ctccaagaat cgccaggcgc tgatcgagat cgaggagaaa gagcttcgcc gcatggagga
     2281 agatttcatc aaacaggcca gcgtgctcag tccggccgcg aagaatgaac gggaacaggt
     2341 cttcagacgc cgtatggcgg agtatcagca gaaagccgcc gagctgaatc gagaagtgca
     2401 ggaaaaacaa aaggacgttc tcgaagcgtt ccgtgataaa gtggaggccg tcgtcgccaa
     2461 ggtggcgaaa aggaatggct tgcaagtcgt catcgataaa ggcaaagggg gtcctgcgat
     2521 tttcggagcc gaagagttgg atctcacgaa tcaagttatc gaagagttca acagagaata
     2581 cccgtgaagc cggaccgagg gatggtcgag gaggaatatt catgaaacgg aatacacgag
     2641 tctttctggc gagtatcggc tccatgtggt ggatggtgtc gtcgatggcg ctggcggcgg
     2701 atccggtgaa ggtcggtgtc atggatcagc aactcgtgat tgaaaaatcc aaagcaggga
     2761 aacgtgccct cgaggagtta aaggcctatt ccgcgacgag gcagaagatc atcaactccg
     2821 acgatcagga attaaaagaa ttggaacaga cgatccagga tgcgaaactg agcgaggcgt
     2881 ccaagcaaga aaagcagaac cagttccagg cgaagctcga ggcgtatcag cggcgtctgg
     2941 ctgaattcaa tcgggagatc cagcagaagc agcgcgaaat ggtgaccgag tactccaaga
     3001 aggtccagga tgccgcgcaa gtggtggcgc agcgggaggg ctacatcgcg gtgatcgatc
     3061 gaggcaatga agccgctatc aggattgtaa tctatcacca gccggccttg gatgtgaccg
     3121 aacacgtcgt gaaagaattc gataagcaga acaaataggg tggcgcagtt tgctagcgcc
     3181 ttgtgcttgc ccaacgcgcc cgcgagggtg gaacaagttc tgtttggcga agctcgtctg
     3241 gctaaagcgc acgtcgcggc ccatcgcttg cgaggcaacg gtgggtcggg cgggaccaag
     3301 agatgtgcag ggtcaggtgg gtgagaaacg tgcaacagcg agggactcaa gcagctcgcg
     3361 cctagagaga cgtatcgagc aagcttgcca tttggaggtt ccgcgatgac cgtgatggaa
     3421 caagtcgaga tccaggcgct cttacctcat cgctatccgt ttttgctcgt cgaccggatc
     3481 ctcgaactcg aaccggatcg ccggatcgtt ggcatcaaga acgtgacggt gaatgagccg
     3541 ttcttccaag gacatttccc gggacggccg gtcatgccgg gtgtcctgat cgtggaagcg
     3601 atggcgcagg tcggcggact cttggccttc aaatcgttgg ggccggtagc gaagcccctt
     3661 gtatatttga ccggcattga cggggcaaaa tttcgcaagc cggtggtgcc gggtgatcgg
     3721 cttcgcattg aaatcgatgt cctcaaaaaa cggtctcctt tctggaaaat gttaggcaag
     3781 gccttcgttg agtcggaggt cgtctgcgaa gcggaagtca ctgccatggt gtcggaagaa
     3841 aaaagcgcca gtgggggtcc atcggccatg tgaccggtgg tgagaagggt tgagagtgaa
     3901 gacaagtctc tgacgtgtcg ttcgtaggag gtactgcgtg gagattcatc cacaagccat
     3961 tgtccatccc aaggccacgc tcgccgatga cgtgagcatc ggaccgtttt gcgtcgtcgg
     4021 agaacatgtc acgattggac aaggaactcg cctgctctcc catgtcaacg tcgaaggctg
     4081 gaccaagatc ggtgcgcgtt gcgagctcca ccccttcgtg tcgatgggcg ggccgcctca
     4141 acatttgggg tatcagggtg agccgactca ggtcgtggtg ggggatgaca acatcttccg
     4201 tgagtatgta accgtcaatc gcgcgacggt gcagggcggt ggaatcacgt ctatcggcga
     4261 caagaatttt ctcatggcct attgtcatgt ggcacatgac tgtcgtctgg gcagccatct
     4321 catcatggcg aacgcagcca gcctggcagg gcacatcgcg atcagcgatc atgcgatcat
     4381 cggcgggctt accggcgttc atcaatacgt ccgaatcggc gcctatgcca tggtgggcgg
     4441 atgctgcgcc gtgacccaag acgttccgcc gttcatgcgt gtggccggag ggtatcccgc
     4501 ccggatgtat gggttgaatt ccgtcggatt gcgccggcac gggttctcgg ccgagcggat
     4561 tgcggtcctc aagcgcgcgt acgacatttt gttccgatcg gggcatcgga tggccgaagc
     4621 ggccaaactt gccaaacggg agttcggcga tcagccggac gtgatggccg tactggcctt
     4681 cctggaagga accaagcggg ggatctgccg ttccgtcggg aaagaccagg cagacgagga
     4741 gtagccggtg acgcaggcgc ctctagtcgg cggcggtgag cggctgggtt tgattgccgg
     4801 caatggccgg tttcccatta tcttcgcgga caatgcgagg aagcttggct accacgtttc
     4861 ggctgtcgcg cacgaaggag aaacagatcc cgagctagcg aaccatgttg atcgcatcca
     4921 ctgggtcaag atcggccaac tcaataagct cattcatgcc tttaaggaag acggggtgca
     4981 tcatgccgtc atgctcggcg gtataaagaa gacccacgtg tttacgactg tacgtccaga
     5041 tttccgcacc ttggcgctgg ccacccgatt ggctctgtgg aaagacgatg acatcttgcg
     5101 cgagttcgcc aaggaactcg aaggtgaagg catcacgata tgcgaatcga ccttcggcct
     5161 tgaagggatt ctcgcggaag aggggacgct gaccagccgt aaacctactg aaaaagaatg
     5221 ggaagatatt cgctatggat gggacgtcgc acaagagatc ggccgcctcg acattgggca
     5281 atgcgtcgtc gtcaaagacc gcatggtcgt cgccgtggaa gccgttgagg gtacggacga
     5341 agcgatcaaa cgtggcgggc aattggccaa gggcggcgcc gtcgtcgtca aacgttgtaa
     5401 gccgcagcaa gatttacgat tcgaccttcc ggcggtcgga ccccggacca tcgatgtgat
     5461 ggcatcagtc agtgcggccg tgttggccgt ggaggccgga cgcaccatcc tgctggatcg
     5521 cgaagtgacc ttgcagaagg cgcaatcaaa tcggatagcc gtagtagggt tggtcaaaac
     5581 agaccagagc ggagggtcgg aggcgcggtg acaccgttga gagcgggtgt gatcggagta
     5641 ggacatctcg gccagcatca cgcacgcctt tacgcctccc tccccgggtc ccgcctcgtg
     5701 gccgtgaacg accaatccat cgaacgggct cacaccattg cggaccgaca cgaggcccgc
     5761 gtctgcaaat ccctagacga actcctgtct ctcgttgatg tcgtcagcgt cgccgtaccg
     5821 acttcctccc attacgccgt cgcgaaagcg tgtttgctgg ccgggaagca cgtgctggtg
     5881 gagaagccga tcgctgtcac gatggcagaa gcccaggaac tggtccggct ggcagaggac
     5941 aagcactgcc ggctgcaagt cggacacagc gagcggttca atcctgtcat gcaagtgatg
     6001 cggccgcata tcgggcgccc ggtcttcatc gaatgtcacc gcttgagcag cttcagtgag
     6061 cgcggcaccg acgtcgacgt gattctggat ctcatgatcc atgatttgga catggtgctc
     6121 tctttcagtc cgggaccggt cgaagacgtg caggcagccg gtgtaccggt cctctcgtcg
     6181 acgatcgaca tcgccaacgc tcgtatccag ttcaagagcg gctgtgtggc aaaccttacg
     6241 gcaagccgtg tatcgacgaa taagatgcga cggttgcggc tgttccagcg tgacaactac
     6301 gtgtcgatcg atttccagac caggcagggg atgatctgcc gacgggaggc cggctcaggc
     6361 ctcaagccga ccatcgactt cgaacaactg caaggcggag aagaagagcc gctgaagctc
     6421 caactggagt cgtttctcac cgccgcggta acgggagcga ggcccacggt ttccggtgaa
     6481 gacggggcgg cggcgctgga tttggctcat caggtgttga aggcgattga tgtgtttgtt
     6541 cggcgcaatc aagagaaagg ctgagccgtg cccaaggcgg aattcagtta actcatgccg
     6601 cggatcttga ttgtgaccgg tgaagcctca ggcgatctgc atggagccca tttggccgca
     6661 gcgattgtcg cgcaagaccc cacagcagag gtcattggca ttggaggagc cgctatgcga
     6721 gcagccggcg tgacgctcgt ccccgatgtg cctcagttgg atgtgatggg gttgattggt
     6781 ttatccgctg tccgcacatt gatgggacgc ctgagcgcta tccggcgcat cttgaggtcc
     6841 gagccttggg atctcgtggt cctcattgat aatccgggac tcaacctgca tttcgcccgt
     6901 gtcgccaagg ctgctggccg ccgggttctc tactatatcg ccccgcaagt gtgggcctgg
     6961 cgtccaggca ggatgaaatg gatccaacgt agggtagatc acgtggtcgt catccttccc
     7021 ttcgaagctc gtctctatga acgtgcaggt gtgcgctgca cgtttgtcgg ccacccgttg
     7081 ctcgattcgg tcgccccgca ctacgataag accacactac gccgacggta tcagctgccc
     7141 gagtcggctc cggtcgtcgg cctcttgccg gggagtcgag cggccgaggt gacgatgctg
     7201 ctccctctgt tgctggagac ggctgcgcag gccgccgctc gggagccgaa cacgcaattt
     7261 ctcctggcac aggctgcgtc gattgacgat aatttgctcc atcgtctgct tgaggacagt
     7321 ccggttcgcg tgcagattgt caaggaccag gccagcgaag tgatggctgc gtccgatctg
     7381 ttagttgtcg catcaggcac ggcgacgctc caagcggccg tgatcggaac acccatgatc
     7441 ctagtgtata aaaccacgct ccccacctac tggctcgccc gcctgctgat tcgggtcaag
     7501 tggatcggcc tcgtcaatct ggttgccggg cgaacgatcg tgcctgagct cattcaggac
     7561 gaggccactg ccgaacggct cacacgggaa gtggggcacc tgctgagcga ccagcaggcg
     7621 tataatcgca tgaaagacgg attgcgcgag gtgcgacagg cgctcgggga acctggtgcg
     7681 tcgcgacgag ccgcgcgcgt ggtgcttcaa gaatgtcgag cgtagaccgc ttcatcagac
     7741 tcgttcgtta tctcaagccc tatcgtttga ggctgattgc ggcatttatc tgttccgcac
     7801 tggttgcggc gttttcaggg gcctatgctt ggctggtcaa gccggtactg gatgagatct
     7861 ttatcaacaa gaatgagagc ttgctcctgg tgctgccatt ggcactgttt gcggttgccg
     7921 tcctgaagag cgcgttcagc tacggccaga actatctgat gaattatgtg ggcaaccagg
     7981 ttgtcaccga tattcgccaa gagctattcg gcagaatgat ccggctgccg gtcaagtatc
     8041 acgattccaa cacgtccgga cgcctggtct cgcgcgtcat caacgatgtc accttaatgg
     8101 ccaatgccgt cgctggcgtg ctcaaagacg tgtttcaaca ggggctgact tttgtcgtca
     8161 tgctcggcgt gatcttctat cagaactgga aactgggcgg gatgtcggtc attgtgatcc
     8221 ccctcaccgt cgtgaccatg atgcggatgg gaaagcgact ccgctcgctc ggaaaaagcg
     8281 gccaagaaca gatgggcgat atggcgtcta cactccagga aacgctggcc gggatccgca
     8341 tggtcaaggc gtacggcaga gaagcggccg aggcggagcg ttttaaggag agtaacaagg
     8401 cgttcttcgg tacgacgatg aaggcaattc aggtgtcttc gctgggttcc tcgcacattg
     8461 aggtgatcgg cgtcgtcggt gtggcggtga tcatctggta cggcggctcc ctcgtcatcc
     8521 acggagagat gacgccggga gcgtttttct cttttttggc ggcaatgttc atggttttta
     8581 cgccgctccg ccggctctcc ggctcaaaca acaccattca acaggcgctg gcggcggccg
     8641 agcgggtctt cggcgtcatc gatctcgaaa cggaggaaag tacggatcga ggacggctgg
     8701 tgatgcagcc gatcgctcgc tcagtcgtct atcaagacgt gacgtttctg tatgaaggtc
     8761 aggcaatccc cgcattgacc gatatcgatc tggtcattca agccggggag atggtcgcca
     8821 ttgtgggaag cagcgggagc ggcaagacga ccctggccaa tcttcttcca cgcttttacg
     8881 acccgacagc gggaagggtg ctgatcgacg gagtcgacat ccaatccttt acgctggctt
     8941 cgctgcgggc gcagatcggt atcgtgtctc aggatgtcgt cttattcgac gacacagta
//
LOCUS       DASTQY010000503         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_337403, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000503 DASTQY010000000
VERSION     DASTQY010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337403"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VFR82_16095"
     CDS             <1..349
                     /locus_tag="VFR82_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4503172.1"
                     /translation="DELLSRSQIEFDSNQATLTARGQAVLDRLIPVLKEAPRTSIEIG
                     GHTDGFGAADYNKELSRRRADAVRQYLMAHGVAHDLTAVGYGASKPRAAGITRAELQR
                     NRRIELRVLKTGE"
     gene            349..1524
                     /locus_tag="VFR82_16100"
     CDS             349..1524
                     /locus_tag="VFR82_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4503173.1"
                     /translation="MIALISQILGCLLVAAAIGFAVGWLLRNQSAQSDEHQLTDRETE
                     LRIKGQALDTAIYELKVKTSTLSALESKVSSLESLDRSAQHELASRQERIDRLQQELK
                     VSQTRMASLETEEQARRERLAEQETMLSAYANEARQANAARTQSEETLALKEQELLEL
                     QQRLVESDNRLAEIDRLRSQLTELEPAQGRVHWLEVQLSEKDAQHRRALHALENDLSA
                     RDRRLAELGQQAQLLRDRDHQIAGLELRVAQLQALQAEMAGQAKTMGEQEAEISRLRK
                     RLGEFRAALRGRDDGSPVLVRPNGPANQLTLQIPAPKTSTPPRKDDLKKIPGIGPAIE
                     RALNKMGTFTYVQIAKWKDNDIARVARKLDTVPGRIKRDNWIAGAKRQHREKYGEKI"
     gene            1590..>2491
                     /locus_tag="VFR82_16105"
     CDS             1590..>2491
                     /locus_tag="VFR82_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU4503174.1"
                     /translation="MSASFVHLHLHTQFSLLDGANQIEPLIQQVKAFGQPAVAMTDHG
                     NMFGAIEFYRKAKAHGVKPIIGCEAYMALGSRHAKKDSGLAHNDYYHLILLARNVTGY
                     QNLIKLVSKAYLEGFYYKPRMDKELLKEHHEGLIALSGCLSGEIPYLIGQRDMTGALA
                     VAQEFQEIFGRDQFYLEVQANGLDHQRVANAGLIEIHKKLGIPLAGTNDCHYLRKEDA
                     RPHELMLCLQTGKTLSDPARMKFDTDELYVKSTEEIAPAFAEFPGAVTNTCRIADHCE
                     LELALNTTHLPQYKAPEGYTRESYV"
BASE COUNT          592 a          749 c          722 g          428 t
ORIGIN      
        1 ggatgagctg ctctcccgca gccagatcga attcgattcc aaccaggcga ccctgacggc
       61 ccgaggtcag gcggtgctcg atcggcttat tcccgttttg aaggaggcgc cacgcacgtc
      121 catcgagatc ggcggccata cggacgggtt cggcgcggcc gactataaca aggagttgag
      181 ccgccgtcgc gccgatgccg tgcggcaata tctgatggca catggtgtcg cgcatgacct
      241 gaccgctgtc ggctacggcg ccagcaaacc ccgcgccgcc gggattacgc gagccgaact
      301 tcaacgcaac cggcgcatcg agcttcgtgt actgaagacg ggcgagtgat gatcgcactt
      361 attagtcaaa tcctgggatg tcttcttgtg gccgctgcca tcggcttcgc tgttgggtgg
      421 ctcctccgga accagtccgc acagtcggac gaacatcagc tgaccgaccg cgagacggaa
      481 ctgcgcatta aggggcaggc gctcgacacg gcaatctacg aactgaaggt gaagacgtcg
      541 acactgtcgg cgttggagtc gaaagtctcc tcgctcgaga gtttggatcg ctcggcccaa
      601 cacgagctgg cgtcccgcca ggagcgcatt gaccggctac aacaggaact caaggtaagc
      661 cagacacgca tggcgtcttt ggagacggag gaacaggcca gacgtgaacg gctcgccgaa
      721 caggaaacca tgctctctgc gtacgcgaac gaagctcggc aggccaatgc agcccgcacc
      781 caatcggagg aaacacttgc gctcaaagaa caggagcttc tggagctgca gcaacggtta
      841 gtcgagtcag acaatcggct tgccgagatt gatcggctca ggagccagct gacggagctc
      901 gaaccggcac aaggacgtgt ccactggttg gaagtccaac tcagcgaaaa ggacgcgcag
      961 catcgacggg cgttgcacgc cctcgagaat gatctctctg cgcgtgatcg acgtcttgcc
     1021 gaactggggc aacaggcgca gctgctccgc gatcgtgacc atcagatcgc cggactggag
     1081 ctgcgcgtcg cacagctgca ggctctgcaa gcagagatgg ccggtcaggc gaagacgatg
     1141 ggcgaacagg aggcagaaat ctcgcgcctt cgcaaacggc tcggtgagtt ccgtgcagcc
     1201 cttcgtggac gggacgacgg cagccccgtg cttgtccgtc ccaacgggcc ggccaaccaa
     1261 ttgactctcc agatccccgc gccgaaaacc tccactccgc cacgcaaaga cgacctcaaa
     1321 aaaatccccg gcatcggtcc cgccatcgaa cgcgcgttga acaagatggg gaccttcacg
     1381 tatgtccaga tcgccaagtg gaaagacaac gacattgccc gcgtggcgcg gaaactggac
     1441 acggtgcctg gccgcatcaa gcgggacaat tggatcgcgg gtgcaaaaag acagcatcgc
     1501 gagaaatacg gcgaaaaaat ttagcgccga cgccgtcgcg tgaatctgtg cgttgacttg
     1561 ccgccatcgc ccccctagaa tccgccctca tgagtgcttc gttcgtgcac ctgcatttgc
     1621 acacccaatt cagtcttctc gacggcgcca accaaatcga accgctcata caacaagtga
     1681 aagccttcgg acagccggcc gtggccatga cggatcacgg caatatgttc ggcgccatcg
     1741 agttttaccg gaaggccaaa gcgcacggcg tcaagcccat catcggctgc gaagcctata
     1801 tggcactggg cagccgccac gccaaaaaag acagcggact tgcgcacaac gattattacc
     1861 acctcatcct gctcgccagg aatgtgaccg gctatcaaaa tctgatcaaa ctcgtcagta
     1921 aagcatatct tgaaggattt tattataaac cgcggatgga caaggagctc ttaaaagaac
     1981 accatgaggg tctcattgcc ttgtctggct gtctcagcgg agaaattccc tatctgatcg
     2041 gccagaggga catgacgggg gccctcgcgg tcgcccaaga atttcaggag atcttcggcc
     2101 gggatcagtt ctatctggag gtgcaagcca atgggctgga tcaccagcgg gttgcgaatg
     2161 cgggtctgat agaaattcat aaaaagctcg gcattccgct cgccggaacg aacgattgtc
     2221 attatctcag gaaggaagac gcccggccgc acgaactcat gctgtgcctg caaacgggca
     2281 agaccctgag cgacccggcc agaatgaaat tcgacaccga cgagctgtac gtcaagtcga
     2341 cggaggaaat cgcacccgcg ttcgctgagt tcccgggagc ggtcaccaac acctgccgaa
     2401 tcgccgacca ctgcgagctg gagctggcgc tcaacacgac ccatctgccg cagtacaaag
     2461 caccggaagg ctatacgcgc gaatcgtatg t
//
LOCUS       DASTQY010000504         5266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2237 k141_538268, whole
            genome shotgun sequence.
ACCESSION   DASTQY010000504 DASTQY010000000
VERSION     DASTQY010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960704
            Sequence Read Archive: SRR25158481
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Pseudomonadati; Nitrospirota; Nitrospiria; Nitrospirales;
            Nitrospiraceae; Nitrospira.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 00:19:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,226
            CDSs (total)                      :: 3,184
            Genes (coding)                    :: 3,174
            CDSs (with protein)               :: 3,174
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538268"
                     /isolate="SMAG_U2237"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="39.929738 N 116.401988 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /gene="carB"
                     /locus_tag="VFR82_16110"
     CDS             complement(<1..1543)
                     /gene="carB"
                     /locus_tag="VFR82_16110"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU4503175.1"
                     /translation="MPKRTDIQSILLIGSGPIVIGQACEFDYSGTQACKALKDEGYRV
                     VLINSNPATIMTDPEFADRTYVEPITLDVIEKVIERERPDALLPTMGGQTALNATMGL
                     VKRGTLDKYGVALIGASAEAIHKAEDRDAFKQAMQRIGLRVPESGTAHSREEAIRVLD
                     RVGFPAIIRPSFTMGGTGGNIAYNREEFERLIEWALAMSPVSQVLIEQSLIGWKEYEL
                     EVMRDLKDNVVIVCPIENLDPMGVHTGDSITVAPAMTLTDKEYQRMRDAALRIIREIG
                     VDTGGSNIQFGVNPDTGELVVIEMNPRVSRSSALASKATGFPIAKIAAKLAIGYTLDE
                     ITNDITGVTKASFEPTIDYVVVKIPRFAFQKFKGADPTLTTQMKSVGEAMAIGRTFKE
                     SLQKAIRSLELELNGLASRVGLDRGIPEDFDRQGALERIRQQLRSPIAERIWYVADAI
                     RLGMSNDELFALTKIDAWFLDQLRDLMRVEQRLVEQASGQASLDDALLWEAKEQGFSD
                     ERIAQL"
     gene            complement(1550..2533)
                     /locus_tag="VFR82_16115"
     CDS             complement(1550..2533)
                     /locus_tag="VFR82_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU4503176.1"
                     /translation="MALSAKDTHRSDSDSLSQVMAAPVGRRAILAWGARGALGVWTAL
                     GTGSALTVLSALSGCYRAPGTARDQLIFFSEEKELQFGLSAYREVLRQARLSENAEIN
                     EQIQRVGRKIADVANKPEYQWEFAVIEDDKMVNAFCLPGGKVAVFTGILKHTKTDAGL
                     ATVMGHEIAHALQRHGVERMSRSIIDQIAQLGALGAAAAGHSSGGAIQGLLGAYGVNV
                     SLPFNRKQESEADYIGLRLMSQAGYDPREAVPFWERMSGCPRQMIDKLCFRSQHAIPE
                     FLSTHPSDVTRINQLEAWLPEAMRYYHGAEGGPVPAPAPYKPAIGPLPQAS"
     gene            complement(2541..3509)
                     /gene="sppA"
                     /locus_tag="VFR82_16120"
     CDS             complement(2541..3509)
                     /gene="sppA"
                     /locus_tag="VFR82_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEU4503177.1"
                     /translation="MDKIQRTVFGIFVVLSVFTQHGCVTVNLLEPPGPVQEVQLSGSG
                     DSKVLLLDLSGVISSQDKEGWLPQPNLLATFKEELTKASKDEKVKAVVLRINSPGGTV
                     NASDILYHELKTFKENRKIPVIASMMDVAASGGYYLAMATDAILVHPSTVTGSIGVIM
                     LTVNAKGLLEKVGVEANAITSGPRKDMGSPFRSMTTDEKEIFQGVIDSFYQRFLTVVQ
                     AGRPQLSAEQIKKLADGRIYSGDQAKAAGLVDDIGYLDEAIDLAKKNANLKEARVVTY
                     RRHGEYQNNIYSRVFGSGNGLSSLASMDLLSIVRGGSPQFMYLWMP"
     gene            complement(3502..4674)
                     /gene="carA"
                     /locus_tag="VFR82_16125"
     CDS             complement(3502..4674)
                     /gene="carA"
                     /locus_tag="VFR82_16125"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU4503178.1"
                     /translation="MKRALLALADGKVFEGRALGFEGETVGEVVFNTAMTGYQEVLTD
                     PSYKGQIITMTCPHIGNYGVTPEDVESTRIWAEGFVVKEASRGASNWRSRQGLHDYLK
                     TARIVGIEGLDTRALTRHLREHGSQQGLISHVEGNAERAVEKARQAPDIVGRDLAAEV
                     TCRNKYRWTDGSGEWLPASHGSAAASTRRWKVVAYDFGVKFNILRRLVDVGCDVHVVP
                     ASTTAEQVEALAPDGVFLSNGPGDPEGVGYAIKAMRQLIGRRPIFGICLGHQILGLAL
                     GFKTYKLKFGHHGANHPVINLRTKQVEITSQNHNFAVQVPELPEVIPDRPPLLDSDFG
                     RVAVTHMSLNDRSIEGLACLDQPVFSVQYHPEASPGPHDSAYLFTEFVQLMEKSHG"
     gene            complement(4671..5186)
                     /locus_tag="VFR82_16130"
     CDS             complement(4671..5186)
                     /locus_tag="VFR82_16130"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEU4503179.1"
                     /translation="MHYGWGLACALSVEYLAIGVWVGGLIVLVGAVIPAVFNTFGGQD
                     SGGFFLTKAFEGYNRLVVVALIILVSAMGYRRWQAAPRATVSRGEWVLVSVMGAIAGL
                     IILVLHPHAAALQAQAFALKDEQARKAAFEAFFRFHMPIRSLYVINVVLGVWLLALKA
                     KRSIQQERVPA"
BASE COUNT         1076 a         1656 c         1379 g         1155 t
ORIGIN      
        1 tcagctgtgc gatccgctcg tccgaaaacc cttgttcttt ggcttcccag agcagcgcgt
       61 cgtccaagga tgcctgcccg ctggcctgtt ccaccagccg ctgctcaact ctcatcaggt
      121 cccgcaactg atccaggaac cacgcgtcaa ttttagtcag cgcaaagagc tcatcgttgg
      181 acatccccaa ccggatggca tccgcgacgt accagatccg ttcagcaatg ggacttcgca
      241 gttgctgacg gatccgttcc agggcgcctt gtcgatcgaa atcttcggga atgccgcggt
      301 ccaagccgac cctggaggcc agaccgttca gctccagttc aagcgaacgg atcgctttct
      361 gcaacgactc tttaaacgtc cgcccgatcg ccatcgcctc gcccaccgat ttcatttgcg
      421 tggttaatgt gggatcggcg cctttgaatt tctgaaaggc aaagcgggga atttttacga
      481 cgacgtagtc gatcgtcggc tcgaacgagg ctttggtgac gccggtgatg tcgttggtga
      541 tctcgtcgag tgtgtacccg atcgcgagct ttgccgcaat cttcgcaatg ggaaagccgg
      601 tggctttcga cgccaaggcc gaactccggg acacccgcgg gttcatttca atcacgacca
      661 gctccccggt gtcgggattg acgccgaatt gaatgttcga accgccggta tcgaccccga
      721 tctcccggat gatgcggagc gcggcatcac gcatgcgctg atattctttg tccgtcagcg
      781 tcatggccgg tgcgaccgtg atgctgtcac cggtatgcac gcccattgga tccaaatttt
      841 ctatggggca cacaatgacg acgttgtcct tgagatcccg catcacctcg agttcgtatt
      901 ctttccagcc gatcaacgac tgttcgatca agacttggct gaccggactc atggccaagg
      961 cccattcgat taatcgttcg aattcttccc gattgtaggc gatgttgccg ccggtgccgc
     1021 ccatcgtaaa ggatggccgg atgatggcgg gaaaccccac gcggtcgagc acacgaatgg
     1081 cttcctcgcg gctatgggcc gtcccgctct ccggcacgcg cagcccgatc cgttgcatcg
     1141 cctgcttgaa ggcatctcga tcctccgcct tgtgaatggc ctccgccgaa gctccaatca
     1201 aggccacgcc atacttatcc agcgttcctc gcttcaccag tcccatcgtc gcattgagcg
     1261 ccgtttgccc tcccatcgtc ggaagcagcg catcgggacg ttctcgttca atgacttttt
     1321 cgatcacatc cagcgtaatc ggttcgacgt atgtccggtc cgcgaattcc ggatccgtca
     1381 tgatggtcgc cggattgctg ttgatcagga ccacccggta gccttcatcc ttgagcgcct
     1441 tgcacgcctg ggttccggaa taatcgaatt cacaggcctg cccgatgacg atgggccccg
     1501 atccgatcaa caggatgctt tggatatcag tgcgtttcgg catgaagaat taactggcct
     1561 gaggcaacgg cccgatggcc ggcttgtagg gggctggggc cggtacaggc cctccttccg
     1621 ctccgtgata ataccgcatc gcttcgggta accacgcctc caattgattg atgcgagtca
     1681 catcggatgg gtgggtcgag agaaactcag ggatcgcatg ttgcgaccgg aaacacaact
     1741 tgtcgatcat ctgccgcggg caaccgctca tgcgttccca gaaggggact gcctcgcgtg
     1801 gatcataccc ggcttgcgac atcaatcgca ggccgatgta atcggcttcc gactcttgtt
     1861 ttcggttgaa cggtagcgag acatttacgc cataggcacc gagcaatcct tgaatggcgc
     1921 ctccgctcga atgacctgca gccgccgcgc cgagggcgcc cagttgggca atctggtcga
     1981 tgatgcttcg gctcattcgt tccaccccgt gccgctgcag ggcatgggca atttcatgcc
     2041 ccatcacggt cgccaaaccg gcgtcggtct tcgtgtgttt cagaatgccg gtgaacacag
     2101 cgacttttcc ccctggtaaa cagaaagcat tcaccatctt gtcgtcttcg atgacggcga
     2161 actcccactg atactcaggt ttgttcgcca cgtcggcaat ttttcgaccg acccgctgaa
     2221 tctgttcgtt gatctccgcg ttttcactca ggcgtgcctg ccgcaatact tcccgatacg
     2281 cactgagccc aaactgcagc tctttttctt ccgaaaagaa aatcagctga tcgcgggccg
     2341 tgcccggagc gcgataacac ccggagagcg cgctgaggac cgtaagcgcg cttcctgttc
     2401 ccagcgcggt ccacaccccg agtgcgccgc gtgcgcccca ggccaggatg gctcggcgtc
     2461 cgaccggcgc cgccatgact tgtgacagcg agtcggaatc cgatcggtgc gtatcctttg
     2521 ccgacaatgc catgtcactc ctacggcatc cacagataca tgaactgtgg gcttccaccg
     2581 cgaacgatgg acaagagatc catgctcgca agactgctga ggccgtttcc gcttccaaac
     2641 acccgtgaat agatgttgtt ttgatattcc ccatgccggc ggtacgtgac gacacgggct
     2701 tccttcagat tggcattttt cttggccaag tcaatcgctt catcgagata cccgatgtca
     2761 tcgaccaaac ctgccgcctt cgcttgatca ccggaataaa tgcgcccatc cgcgagtttt
     2821 ttgatctgct ccgccgagag ttgcggacgt cccgcttgca ccactgtcaa gaaccgttgg
     2881 tagaacgaat cgatcacccc ctgaaaaatc tctttttcgt ctgtggtcat cgatcggaac
     2941 ggagaaccca tgtccttgcg gggtcccgac gtaatggcat tggcttcgac gccgaccttt
     3001 tccaagaggc ccttggcgtt cactgtcagc atgatgacgc caatgctacc ggtgaccgtt
     3061 gagggatgca ccaggatggc gtcggttgcc atcgccaagt aatagccgcc ggacgccgcg
     3121 acatccatca tggaagcaat gaccggaatt ttcctgtttt ctttaaacgt cttgagctcg
     3181 tggtacagga tgtcggacgc attgacggtg ccgccggggc tgttgatcct caacacgacc
     3241 gccttgacct tctcgtcctt tgatgccttg gtcagctctt ccttgaacgt cgccaggaga
     3301 ttcggctgcg gcagccatcc ctccttatct tgggaactga tgacgccgga caaatccagc
     3361 agcaacactt tgctgtcccc agacccgctg agttgcacct cttgcaccgg gccgggtggc
     3421 tccaacaaat tgaccgtgac gcacccgtgt tgcgtgaaga ccgagagcac gacgaaaatc
     3481 ccaaaaaccg tacgctgtat cttatccatg cgacttctcc attaactgaa cgaattcggt
     3541 aaagagatag gctgaatcat gaggccccgg agaggcttcc ggatgatatt gtaccgaaaa
     3601 gaccggctga tccaagcagg ccagcccctc aattgaccgg tcgtttagac tcatatgtgt
     3661 caccgccacc cgaccaaagt cgctgtcgag gagcggagga cgatcgggaa tgacttccgg
     3721 tagttccgga acctgcacgg caaaattgtg gttctgcgac gtgatctcga cttgtttggt
     3781 tctgagattg atcacaggat gattggctcc atggtgcccg aatttcaatt tgtaggtctt
     3841 gaaccccaac gccaagccga ggatctggtg ccccagacag ataccgaaaa taggccgacg
     3901 accgatcaac tggcgcatcg ctttaatggc atagcccacg ccttcgggat cgccggggcc
     3961 gtttgacaga aaaacaccat ccggcgcgag agcctctacc tgttccgccg tcgttgaagc
     4021 aggaaccacg tgcacatcgc acccgacatc aaccagccgg cgcaagatgt tgaacttcac
     4081 cccgaaatca taggcgacga ctttccaccg gcgtgtcgag gccgccgcgc tgccatggga
     4141 tgccgggagc cattcgccgg aaccatcggt ccaccggtac ttgttccgac aggtgacctc
     4201 ggcggctaaa tcccttccga cgatatcggg cgcctgtcga gctttttcca ccgctcgctc
     4261 ggcgttgcct tcaacatggg agataagtcc ctgctgcgag ccgtgctcac gcaaatgccg
     4321 ggtgagcgct cgcgtgtcga gaccttcaat ccccacaatc ctcgccgttt tcaagtagtc
     4381 gtgaagccct tgccggctgc gccaattact cgcccccctg ctcgcctcct tcacgacaaa
     4441 cccttccgcc caaatgcgtg tcgactcgac atcctccggc gtcaccccat aattgccgat
     4501 gtgcggacag gtcatggtga tgatctgtcc tttataagag ggatcggtca ggacttcctg
     4561 ataccccgtc atggctgtat tgaacaccac ttcgccgacc gtctcgcctt cgaatccgag
     4621 tgcgcggcct tcgaacacct ttccgtccgc cagtgccagc agcgcccgtt tcacgcgggt
     4681 accctttctt gctgaatgga ccgtttcgct ttgagcgcca gcaaccagac gcccaacacc
     4741 acattgatga catacagcga gcggatcggc atatgaaacc gaaagaacgc ctcaaacgca
     4801 gcctttcttg cctgctcatc cttgagggca aatgcctgtg cttgcaaagc tgctgcgtga
     4861 ggatgtagca cgagaataat caaccccgct atggcaccca tgacactcac gagtacccac
     4921 tctccccggc tgactgttgc gcgtggcgcg gcttgccatc gccgatatcc catcgcggag
     4981 accaggatga tgagcgcgac gacgaccaat cggttgtagc cttcgaacgc tttcgtgaga
     5041 aagaatcccc cggaatcctg cccgccgaac gtattgaaga ccgccggaat caccgcaccg
     5101 accaacacga tcaatcctcc cacccagacg cctatcgcca ggtactcaac actaagcgca
     5161 caggccaggc cccatccgta gtgcatgctc acgccgaccc cgccgcttca aacacgacgc
     5221 ggccggccac gatggttgtc atgacgagtc ctttgacttt ccaacc
//
